LOCUS       DAOOMC010000001        64218 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdus sp. isolate AMR_MDS_2685
            NODE_288_length_64218_cov_6.574771, whole genome shotgun sequence.
ACCESSION   DAOOMC010000001 DAOOMC010000000
VERSION     DAOOMC010000001.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864760
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdus sp. (wastewater metagenome)
  ORGANISM  Syntrophorhabdus sp.
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae; Syntrophorhabdus.
REFERENCE   1  (bases 1 to 64218)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 64218)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,729
            Genes (coding)                    :: 2,708
            CDSs (with protein)               :: 2,708
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 7 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..64218
                     /organism="Syntrophorhabdus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_288_length_64218_cov_6.574771"
                     /isolate="AMR_MDS_2685"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2699756"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(87..1688)
                     /locus_tag="PK125_00005"
     CDS             complement(87..1688)
                     /locus_tag="PK125_00005"
                     /inference="COORDINATES: protein motif:HMM:NF024581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HPB36521.1"
                     /translation="MADERQKTSHEMLDISLKPSGDLLTPKPDFRKGTYCYSAPVKHA
                     AYLDLPYVRDWKPNEFDWKLPDKWQEIILDGMRTLLKRHRSFRLFLDICVRCGACADK
                     CHFFVGSGDPKNMPVARAELLRSIYRRYFTFCGKIFGRFAGARDLTEDVLKEWFYYFY
                     QCTECRRCSVYCPYGIDTCEITMMGRELLNLLGLNIQWIVEPASNCFRTGNHLGVPPN
                     AFQQTFEFALDELRELTGIAIEAPINRKGAEILFVAPSADYFASPHWFTFLGYLMLFH
                     EIGLDYTWSAFASEGGNFGLFHSSELAKRLNAKIYEEARRLKVKWILGGECGHMWRTI
                     HQYMDTFNGPADSLEEPVSPITGTTFGNARSTKMVHITEFTADLIYHRKLNLNKSRND
                     KWKVTFHDSCNPARSMGLFEEPRYIITNTCNHFYEMPENTIRERTFCCGSGAGLGADE
                     NMEMRLRGGFPRANAVKYVKEKYGVNIVACMCAIDKASLPPLFDYWVPGTEVCGVHEL
                     VGNALIMTGEKKRTTNLRGEPLEAE"
     gene            complement(1681..2673)
                     /gene="dsrM"
                     /locus_tag="PK125_00010"
     CDS             complement(1681..2673)
                     /gene="dsrM"
                     /locus_tag="PK125_00010"
                     /inference="COORDINATES: protein motif:HMM:NF038037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate reduction electron transfer complex
                     DsrMKJOP subunit DsrM"
                     /protein_id="HPB36522.1"
                     /translation="MNVLNALFAIVLPYAALVAFIIGFMYRVILWACSPVPFHIPTVC
                     GQQKSLPWIRSNKTESPSTTWGIFWRMALEIIFFRSLFRNDRTKLTDREQATYWGNKL
                     LWFGGIIFHWSLLVILLRHLRLFFEPVPRHVTFLQNLDGFFQVFTPTFFVTDALIIIA
                     LSYLFLRRILNPQIRYISLISDYFPLLLILGIAVSGLLMRHIYRVDLVETKRFALGLV
                     SFDPVMPKETGVIFYVHLFLVSVLLAYFPFSKLMHMGGVFMSPTRNLKNDSRAKRHIN
                     PWSYPVKTHTYDEWEEEFREAIREAGLPLEKNKEQPLGFPASLSTIESPEKRRG"
     gene            complement(3547..4743)
                     /locus_tag="PK125_00015"
     CDS             complement(3547..4743)
                     /locus_tag="PK125_00015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB36523.1"
                     /translation="MQTGHTKPSETYAPLYFLASLGSGGLVVTFFMWLMHWTSHPGRS
                     VPVFEDIATAFNSGSLATKAMIIMAVAGIAFYSFLNIKVLIWNLIQFKKWKRTEAYIK
                     IRSSNAETQLMAMPLALAMSVNVCFMLGMVFVPRLWSVVEYLFPLAIIAFVAISILAF
                     DMLGKFLGRALTTGGFSCIANNSFAQALPAFALSMIGVGLAAPAGMSAAPLVSGIGLV
                     LSTFFLVAAMLIGVIALVLGIRSLLENGADIETAPTLSIFIPLLTVTGILVLRQSHGL
                     GEHFAQASGSASRLMMLSQFLSAQLLFALLTWAVLRRLGYAQRYIFGKEASVGSYALV
                     CPGVALAVMIQFWLNRGLVDAGLINRFSVGYWVISALAIAIQLGTMWLVYVLNRKHFG
                     KKVPHN"
     assembly_gap    5334..5433
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(5633..6091)
                     /locus_tag="PK125_00020"
     CDS             complement(5633..6091)
                     /locus_tag="PK125_00020"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HPB36524.1"
                     /translation="MESTGDSVLIINKETSMKHWLLPTFGAFVLWGFWSFIPKITTRY
                     ISPRSAILFEVMGGVLVATMVLFTLKFKPDIHLKGVLLAMTTGGLGFLGALCYLYAVS
                     KGPVSLIAILTALSPVITVTLSVIFLNETISLKQGFGIVLGLAAMILICT"
     gene            complement(6666..7199)
                     /locus_tag="PK125_00025"
     CDS             complement(6666..7199)
                     /locus_tag="PK125_00025"
                     /inference="COORDINATES: protein motif:HMM:NF025719.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsbRD N-terminal domain-containing protein"
                     /protein_id="HPB36525.1"
                     /translation="MNLNATLEEKKNRILQRWISLTVETYPADVVNFLKMEKDRFANP
                     VGFAISTGCEILFHELTCEGNRSKIITALDSIIRIRTVQDFSPSQAVEFIYFLKKAVR
                     EELKINDEEAIKRTKPEILEDLALFETRIDDLASLACKIYDKCREDINRIKMKDKDSA
                     ITIKNRLARVRIPKGEK"
     gene            complement(7382..8335)
                     /locus_tag="PK125_00030"
     CDS             complement(7382..8335)
                     /locus_tag="PK125_00030"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HPB36526.1"
                     /translation="MTRQPITATEVDRLVVTVITDNYYDALRPDTKVSTVFRSRPGSV
                     IHAEHGLSYHIETEVNGVSSTLFFDYGMDLMGMTHNIEVLGIDVGRVNAMALSHGHFD
                     HWGGLTEFLRRYGDRIPKGTPLYIGQETFARRFSIRPSDNEPMDIGKLERAEIDKFGV
                     VDIRELTEPREIIPGGYATGPVEKVTDYEKGNPNLLVKRGDRLEQDLFEGELALFFVV
                     KNRGLVILSGCAHIGIINIVKYVVQISGISKIHAIIGGFHLVNAQTNLIDRYIEDIKG
                     FSPDYIVPTHCTGFEAIVRFAREMPEQFILNTAGTSYTFTS"
     gene            complement(8381..9658)
                     /locus_tag="PK125_00035"
     CDS             complement(8381..9658)
                     /locus_tag="PK125_00035"
                     /inference="COORDINATES: protein motif:HMM:NF041136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0140132 - iron-sulfur cluster carrier
                     activity [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster carrier protein MrpORP"
                     /protein_id="HPB36527.1"
                     /translation="MSTCEQDNQRNPLLDELAMADKLSKIDQVIVVLSGKGGVGKSTV
                     AVNLALSLALRGHKTGLMDVDIHGPSVPKLLHLMNRWPEMKGEEIVPITYESDLLKVM
                     SMGFLLQGTEQAVIWRGPLKYSMIKDFISRVQWGDLDYLVVDCPPGTGDETLSVAQFL
                     KEKAKAVIVTTPQQVATIDVEKCITFCKQLELPVAGVIENMSGFVCPHCGQSTEIFSK
                     GGGELLADRYGIPFLGSIPLDPAIAQSGDEEKPYIYFYSKSATAKVFEDIIERIIFFA
                     KDNSLGIKKESENKTPTATQPGDKDTKENISTLNKEASMKFAIPTHQGKLCAHFGHCE
                     AFALIDTDATGKVINETYVTPPPHEPGLLPPWLSQQGVNCIIAGGMGSRAQQLFAQQG
                     VNVITGAQGEDPKEIVEQYLKGVLQTGANTCDH"
     gene            complement(9655..10536)
                     /locus_tag="PK125_00040"
     CDS             complement(9655..10536)
                     /locus_tag="PK125_00040"
                     /inference="COORDINATES: protein motif:HMM:NF013793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HPB36528.1"
                     /translation="MTDIKEVIVVSGKGGTGKTSVVGPLVSLMTNRVAADCDVDAANL
                     HMILNPVVIEEKEFIGGKKATIDPSRCTSCGTCRDVCRFQAISADYIVDHRQCEGCGA
                     CYFLCPANAIDFPSTVAGSCYICKDKYSQPYVYAELLPGEENSGKLVTMVRSEAKALA
                     EKNGCPLIVIDGPPGIGCPVIASMTGTHVATVVTEPTISGFHDLERIIELARFFKCKT
                     GVIVNKSDINPDFCKRIEDYCITKETAFLGAIPYDHRITEAQIQAKTILDIAPDSETS
                     EIIRTIHKKISKILEEL"
     gene            complement(10539..11408)
                     /locus_tag="PK125_00045"
     CDS             complement(10539..11408)
                     /locus_tag="PK125_00045"
                     /inference="COORDINATES: protein motif:HMM:NF013793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPB36529.1"
                     /translation="MNIAIASGKGGTGKTTIAVSLAYFLATSGQSVQYIDCDVEEPNG
                     HLFLKPKIESRKTAYVMVPKIIEEKCTFCGECSSKCQYNALVSLPDIPPLVFPGLCHG
                     CGLCVHICPENAIIEAKREVGFIEEGTGIEGIACIHGILNVGEVMAVPLIRQVKEGSL
                     ESRVHIIDAPPGTACPLVATISDADIVVMVTEPTPFGLHDLSIAVAVAEKLEIPLGLV
                     INREREQFQPLEKYIDDHGMEILARIPEDRRIAEHYSRGEIIIEALPELRPLFSDLAI
                     NIGKAFGKSVIGV"
     gene            complement(11432..11626)
                     /locus_tag="PK125_00050"
     CDS             complement(11432..11626)
                     /locus_tag="PK125_00050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB36530.1"
                     /translation="MDRVWEAEQEQANVWGLEGEEEEEWVEEDWGRTVNVFAHSVGSG
                     RLTKEGFPVLNRNVQNVEFQ"
     gene            complement(11821..12180)
                     /locus_tag="PK125_00055"
     CDS             complement(11821..12180)
                     /locus_tag="PK125_00055"
                     /inference="COORDINATES: protein motif:HMM:NF014620.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifB/NifX family molybdenum-iron cluster-binding
                     protein"
                     /protein_id="HPB36531.1"
                     /translation="MKIAITSVDGTMEGKLDERFGRARKIIVYDTETKEHVVLDNSMN
                     LNAAQGAGIQAAQNVVKSGAKVIISGHFGPKAFQVVQVAGLEVYSATDMIVADAIKRF
                     EEGKLEKLASADVSSHW"
     gene            complement(12177..12434)
                     /locus_tag="PK125_00060"
     CDS             complement(12177..12434)
                     /locus_tag="PK125_00060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB36532.1"
                     /translation="MTRQTSPGKAGFFPSVTHFKSAWTAHQKITNALEGGMDLRIKDG
                     NLTASDILPGFWQWEKRWAYRAKSLPEKSIKEIISLREDEK"
     gene            complement(12573..13049)
                     /locus_tag="PK125_00065"
     CDS             complement(12573..13049)
                     /locus_tag="PK125_00065"
                     /inference="COORDINATES: protein motif:HMM:NF013923.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HPB36533.1"
                     /translation="MKRVKVNFTVNGEKVSVEVKQYETLLETLRERLCLTGVKESCSI
                     GSCGACTVILDGIPVRSCLVLSPEVEGKSITTVEGLEKNGILHPLQQAFMEKGAVQCG
                     FCTSGMILTAKSLLDRQSKPSKDQIIRTISSNICRCTGYKKIIEAVMSAAEKMVAT"
     gene            complement(13049..13993)
                     /locus_tag="PK125_00070"
     CDS             complement(13049..13993)
                     /locus_tag="PK125_00070"
                     /inference="COORDINATES: protein motif:HMM:NF013134.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HPB36534.1"
                     /translation="MILPEFEYKKPKVIKKALQLFQANRGDAVYLSGGTDLVPRIKQR
                     LEKPSIVIDLKNISSLNKIELSRSWLKIGANVTIYELKNHPFVKDNLPALADSLEATA
                     CETIQMRGTIGGNVLQNTRCLFYNKSDEWRKAKGYCLKMGGQVCNAVKGAKMCFANYT
                     SDNAPALITLETQVRLSGVDGSRQIPLAAIFSGKSDRPFTLQPGEILTHIFIPANKTR
                     GCYLKIRVRDSIDYPLVGAAASLKNGNGCLAIGGIGAKPLHFDFDETEDGWGERIARE
                     AASHVTPVANTVLSPTYRKKMVYVLVKRIVRKLSGEGC"
     gene            complement(13990..16263)
                     /locus_tag="PK125_00075"
     CDS             complement(13990..16263)
                     /locus_tag="PK125_00075"
                     /inference="COORDINATES: protein motif:HMM:NF014758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HPB36535.1"
                     /translation="MENHKVIGQRVVRSDYLAKVTGQACYSADLKLPGMLVAKVLRSP
                     YAHARILAIDVSPALKIPGVKAAVSGQDGYGVKWGVFRYTQDHDMLPTDKVRYVGEDV
                     AAVAAIDEETALEALHAIKVEYEPLPAVFDPVAAMAPDAPQIHDAYPGNINVHVHIDV
                     GDVESAFRESFIVREDTFRAAGEAYAMMEPYAVVAHYNSGYLDFWMPNAGPHVRAKAL
                     SNLLQIPLNRVRVRHIFSGGAFGGRSEVAPGDFITALLSMKSGKPVKLVLSREENAIS
                     SRQVHDIVATIKTGVKQDGTILAKEFKAIYNGGAYSSTGPIAASIPYYVYEEAYRFPN
                     VKYDGYRVYTNKGIRGMYGCHGRAFLAGNEVQLDMIAKDLGIDPMDIRIKNGLTLGEK
                     TATKSWITSGNLAEPTQKAAEKAGWSSKRGKLPKNKGIGMGCVAIMTGFPMGFRSSSS
                     CYVKLNDDGHATVVTGIVDNGQGNDSMAVQIASEILGLPMEDINLVSADTEVTNLDPG
                     AYSQAATFAGGNAVKKACEHARNQILALASEKLNEAVEDLDLQNKMVICRKNPEKKMP
                     ISWITREAFFRAKPIAGIGSYIPKIDFDREWISKPFGQMAGTYSFGTSVAEVEIDEET
                     GKITMPHFVTAHDCGKAINPMAVEGQMEGSVQQAGVAAITEGNLWDEGFLLNPDLLEY
                     KVPLACDMPDIETIIVESNDPEGPFGAKEGGLTVRMNAYSAVACAVSNALGVQFSELP
                     LTPDRILEMIEKKGGDK"
     gene            complement(16707..17129)
                     /locus_tag="PK125_00080"
     CDS             complement(16707..17129)
                     /locus_tag="PK125_00080"
                     /inference="COORDINATES: protein motif:HMM:NF014620.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifB/NifX family molybdenum-iron cluster-binding
                     protein"
                     /protein_id="HPB36536.1"
                     /translation="MLTTENIGFEENRMKICFAVEKDDGVNSSVYGHFGSAPLFIMVD
                     TNQKVATSVKNEDAGHAHGACNPLRSIGGLPIDAVVVGGIGAGALMKLNAAGVKVFRS
                     APGSVRENLDLFEQKKLTELTVNDGCSGHQSGSGCGHH"
     gene            complement(17817..18605)
                     /locus_tag="PK125_00085"
     CDS             complement(17817..18605)
                     /locus_tag="PK125_00085"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HPB36537.1"
                     /translation="MIKIKQNTYKYVYGPVPSWRLGSSLGIDLLSQKSKICTFDCIYC
                     QLGATKTYSNQRIAYVHVDDILRELRSLPPKIPIDFITFSGRGEPTLASNLGIAVKAV
                     KALRPDPIAIITNSSTMDRASVQEDLSLADFVIAKLDAFSAETFEAVNKPSDMIHFEN
                     VVKGLLKFRKNYNGKFALQIMFTEENKKYAGKIASLSRKIAPDEIQINTPLRSCPVPP
                     LSEIELMDIKNHFADLNVISVYEGNKTYVEPISDSDTLKRRGKV"
     gene            complement(18708..19658)
                     /locus_tag="PK125_00090"
     CDS             complement(18708..19658)
                     /locus_tag="PK125_00090"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HPB36538.1"
                     /translation="MVAELVDMVQVDELRITVVVDNHYFTVQNDSPIVERVSSARGVP
                     IHTEHGLSYVVETTMDGEKNTFLFDFGLNFETIIKNLAILNVSLATVNAFGLSHGHLD
                     HWGGLLQTLKAYRRASSEETTLYVGEEAFAPRFSGYPWGSPHYLGQLDVKQISSIQNL
                     KIKEIKLPTEVMPGGYFTGYIDRVTEYEKARTTLLIARNERLEMDDFRGEQAMIVGLR
                     NKGLIILSGCAHAGIVNTVRHAQKITGISDVHAVMGGFHLFDLGTENIKRTVQDIKSV
                     KPQYIIPMHCTGYDAMTLFYKEMPEQLVLSTAGSRFTFVA"
     gene            complement(19666..20355)
                     /locus_tag="PK125_00095"
     CDS             complement(19666..20355)
                     /locus_tag="PK125_00095"
                     /inference="COORDINATES: protein motif:HMM:NF014620.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifB/NifX family molybdenum-iron cluster-binding
                     protein"
                     /protein_id="HPB36539.1"
                     /translation="MKIAVTSNSTSLDDVIEPRFGRCRYYLIVETDTLDFEAVENPSI
                     SLVGGAGIQSAKLMADKGVTHVLTGNCGPNAYQTLNAARINIVVGCSGVIREIIQQFK
                     QGNLANTTQPNVPAHAGAGAPSGGDQYFSPAFSRGLGMGMGRGMGMGRGLGMGMGMGR
                     GMGMGRGMGTDALFNQGSYPGPQVLNRGEGGASTDEELDMLKQQAEALNQQIEQIKNQ
                     ITELEKRRMGK"
     gene            complement(20456..20848)
                     /locus_tag="PK125_00100"
     CDS             complement(20456..20848)
                     /locus_tag="PK125_00100"
                     /inference="COORDINATES: protein motif:HMM:NF036294.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5320 domain-containing protein"
                     /protein_id="HPB36540.1"
                     /translation="MPFGDGTGPTGMGPMTGRGAGYCTGFGVPGSMNIGPGRAFWGFA
                     GGRGGGRGWRNRFFATGFTGWQRAGFGLPVWGNPWFQGFPYRAGAGPIMSDEQELNAL
                     REQAGYLEEAIAGIRQRIEEIEAKDKKE"
     gene            complement(20977..21243)
                     /locus_tag="PK125_00105"
     CDS             complement(20977..21243)
                     /locus_tag="PK125_00105"
                     /inference="COORDINATES: protein motif:HMM:NF036294.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5320 domain-containing protein"
                     /protein_id="HPB36541.1"
                     /translation="MPRGDGTGPTGMGPMTGRAAGYCAGNNAPGFTNLFPRRPLWNYR
                     GNVVPMQRPAYRRWMPFGVRQPYFFGAVGWRCGRGQGRGRRWFW"
     gene            complement(21286..21666)
                     /locus_tag="PK125_00110"
     CDS             complement(21286..21666)
                     /locus_tag="PK125_00110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB36542.1"
                     /translation="MKVAIAHWQDRISPVFDVSDRLCLIQIEGQREVSRENVVLKMRD
                     PFGRAKEVAGLGTNVLLCGAISHVLEKAIMATGVQVIGFLCGDLGAVIPAFLQGHLDD
                     GGFFMPGCYRQRQRHRFRGRRGRR"
     gene            21857..23212
                     /locus_tag="PK125_00115"
     CDS             21857..23212
                     /locus_tag="PK125_00115"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HPB36543.1"
                     /translation="MTDKNARDVILDSINEGVFTVGLDWRITSFNRAAEHITGVSRND
                     AIGRACSDVFHADICEKDCALRRTFVTGEPTVNATAHIISNQGHRVPIRISAAILKDS
                     DGNAIGGVETFQDLSQVEQLQKELKNRHTFEDIVGRSPTMIKLFEILPQIAESSSTVL
                     IEGPSGTGKELFARAIHNLSPRKKKRFVPVNSAALPDTLLESELFGHKAGAFTDAKRD
                     KAGRFVLAHGGTIFLDEIADISPALQVRLLRVLQERVVEPLGSIEPIKVDVRIVAATN
                     KNLAQLVQAGKFREDLYYRIRVVHLTLPALNQRREDIPLLVDHMVAKFNRLQNKDIAG
                     ISRDAMAHLMEYDYPGNVRELENIIEQAFVLCRGGMIELHHLPPELRPVANVTSKGFG
                     PMSMDAMEKHLIIETLQRHGGNRTEAAQDLGIDMSTLYRKMKRFKIDVPGRDGRGKRR
                     Q"
     gene            23410..24285
                     /locus_tag="PK125_00120"
     CDS             23410..24285
                     /locus_tag="PK125_00120"
                     /inference="COORDINATES: protein motif:HMM:NF014046.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ARMT1-like domain-containing protein"
                     /protein_id="HPB36544.1"
                     /translation="MKTSLDCIPCFLRQSLEAARFVSDDHAFHEYILRKVLKIMIDID
                     LMQSPAVVAQWIHRQIREITGQEDPYRNAKERFTEAALILLPEFSAQVEASADPIEAA
                     VRIAIAGNMIDMGPENVLTPEGVRQTVLQAFSEPLYGDLESFKRAIEAAKSILYLADN
                     AGEIVLDQVLIERLPLGQTTLVVRGHPVINDATMADIEAINLNRFLTVTDNGSDAPGT
                     ILDDCSSQFRELFKSADLIIAKGQGNYETLSDEPKAIFFLLKVKCKVIASRVGLDMGT
                     HAILPSGFWAQGTRS"
     gene            24357..25214
                     /locus_tag="PK125_00125"
     CDS             24357..25214
                     /locus_tag="PK125_00125"
                     /inference="COORDINATES: protein motif:HMM:NF022077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HPB36545.1"
                     /translation="MSKKLQNMDDIVRDARMKEEAENITKRLSHIRHKILVMSGKGGV
                     GKSSIAAYLAVSLAGRGYRVGLMDVDLHGPSIPGLLGLKDKLLTTGGPEGKTFPLKYL
                     PKLEVMSIEVLLGDKDAATIWRGPLKGGVIRQFIAEIEWMELDYLIIDSPPGTGDEPL
                     TVAQTIPDAKALIVTTPQEISLADVRKSINFCRKMNLEVLGLVENMSGLKCPHCGKTI
                     DLFKNMGGMTTAKKENLRLLGSLPIEPKVVECGDEGSIDFLGDDSLEYNRAFSQIVDT
                     IVKVMENKE"
     gene            25362..25796
                     /locus_tag="PK125_00130"
     CDS             25362..25796
                     /locus_tag="PK125_00130"
                     /inference="COORDINATES: protein motif:HMM:NF013738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly scaffold protein"
                     /protein_id="HPB36546.1"
                     /translation="MSGKLDDFLTNLQNEIFDDTIKTYGETAYERWRNPLYMKSIHNP
                     DGYARVTGPCGDTMEIFLKFNEGRVSDASFQTDGCGPSQICGSFAAELAFGKNIDELK
                     EITNQTILNIVGGLPEENQHCALLAANTLHDAVDNYLKKRLS"
     gene            complement(25866..26612)
                     /locus_tag="PK125_00135"
     CDS             complement(25866..26612)
                     /locus_tag="PK125_00135"
                     /inference="COORDINATES: protein motif:HMM:NF014582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HPB36547.1"
                     /translation="MDKLILIIIFSFLGSVGSLAGAFLLLIFPDKVRLMLIPSLISYA
                     TGTLLASSLLGMIPKALVSASSSSILLTVLVGMISFFLLEKLVIWRHCHVENCEVHST
                     AGSLILLGDTFHNFVDGIVIAAAFLTSIPLGLTTSFAVIAHEVPQETGDFAILLDSGY
                     SKNKAFMYNLFSSMATLPGSLIGYFCLKSAQAAIPYILAFSAASFIYIATADLIPHLH
                     QKTDFWSGITQFLLLLCGVGTVLFFIICHP"
     gene            27182..27895
                     /gene="prxU"
                     /locus_tag="PK125_00140"
     CDS             27182..27895
                     /gene="prxU"
                     /locus_tag="PK125_00140"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014259907.1"
                     /note="Most members of this family contain a
                     selenocysteine; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_except=(pos:27431..27433,aa:Sec)
                     /transl_table=11
                     /product="thioredoxin-dependent peroxiredoxin"
                     /protein_id="HPB36548.1"
                     /translation="MVEQKDGCVKPSRGPIGIPKAETGDIVQTNQKEGGFMIAKVGKP
                     APDFEASAYIDGGFKNITLSEFKGKWVVLCFYPGDFTFVUPTELSAVAVKHKEIEELG
                     VQVLAMSTDSRFIHKIWQENELSKMVEGGVPFPMLTDAGGLIGRVYGVYDEEAGVDIR
                     GRFIIDPDGIIQAMEVLTPPVGRNVSEFIRQIKAFQHVRATGEATPSGWQPGKVTLTP
                     GPDLVGNVWKVWKPEMAFE"
     gene            28081..28851
                     /locus_tag="PK125_00145"
     CDS             28081..28851
                     /locus_tag="PK125_00145"
                     /inference="COORDINATES: protein motif:HMM:NF015949.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF364 domain-containing protein"
                     /protein_id="HPB36549.1"
                     /translation="MDQSTDILPLEIAKSRLEEIIRENDLNDSDISVLVKTLSPEEAI
                     GVPGRRDFPIVEGRERVIEADFQGSKAHAFTDSPREFIGKLKDVISLPLADSGERAVF
                     IATMNAVLKYCKIIETTLHCKDEDPEKCAIEISSHIHNTLRAKKVGLIGLNPAILEAL
                     SKTFGPENVRVTDLNGKNIGSVKYGINVWDGKKMTEKLVEESELVLMTGTTLVNGSFE
                     KIWSTVQQYKREYLVYGVTSAGVCSLAGVNRICPYGRK"
     gene            29156..30460
                     /locus_tag="PK125_00150"
     CDS             29156..30460
                     /locus_tag="PK125_00150"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HPB36550.1"
                     /translation="MERSGEHRELLKQGRQVAITATIVIFLLAVAKFVIGYLFQSQIL
                     IADAYHSGVDVLAIFASWFGLQLASRKKSAKFPYGLYRAETFVTLAIGAFITWAGIQN
                     LLEGYKKLYILATDQAFPLMPVIVSGASVIVSFLVAKMERRAGVFINSGALKANASDA
                     FLDTGTSVVVLSGILLAHAKIPYIEGLIVMFIAVLIIRLGIMNVWTPMLILLDANLDP
                     RLQVAIENRIALIDGVKRVNDVKIRQSGPFRMVECNIATKPSVSVFKAHELASTVEKI
                     VTDEHREIESVFVHIEPVQQHTLSAIIPVKEIDGMNSRVHGHFGRAPYFVILKLHDTG
                     TAEIEDFYYNEFLEKKDQIHIAVKVIKVIIKHDLDIVFTTKIGEIAFHMLKDNFIDIF
                     GTEEDITVGEVVKQFREGRLQMIAGPHPAEESEIRHAKEDFK"
     gene            30590..31639
                     /locus_tag="PK125_00155"
     CDS             30590..31639
                     /locus_tag="PK125_00155"
                     /inference="COORDINATES: protein motif:HMM:NF014774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heterodisulfide reductase-related iron-sulfur
                     binding cluster"
                     /protein_id="HPB36551.1"
                     /translation="MYTLKFDIEKCHNCQTQACLTRCQHLDIDREVAQREIRKIARGE
                     DSFILHDCLTCYACEEYCTMGNHPFYLIVEQQEALNIPPVPRPLIQRAVNLGVPFRGE
                     PEIGEVHGPVLNMAAFSDLIGLIQGRLFEGLTVISTDDRKMFHYFCQLMYLHYARSSV
                     IKERLPGVINTINRHKPTEVICFHDECYGTYTSYCPAVGIDVPFTPIHFFEFLHRRLT
                     ELKEVIRPVGFKVAYQRPCSSRLSPDKHHFVRDIFELIGARYVEREYVDENALCCAGA
                     IQGQRREGSRKRAIEMQRKNINDMKKAGAEVCIFNCPACFQTLGSLLSAEDIKPMYMS
                     DLCRLAIGEKPMEWR"
     gene            31644..33002
                     /locus_tag="PK125_00160"
     CDS             31644..33002
                     /locus_tag="PK125_00160"
                     /inference="COORDINATES: protein motif:HMM:NF013714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HPB36552.1"
                     /translation="MDSIYEALVNIVGKEYVSNQPEELYIYSFDLGTAEPCQPQYVVS
                     PRATEHIQGIVRLANKEKIPIVPLGGGLSLAGLAVPLKGGILLDLKRMDSIIEVNENA
                     RYAVVECGVSQGQLTSYLEKNYPRLTHSEPGAPPAATLAGNVMIHGQGDLAQPYGFNS
                     DLVNGLEVVLPTGEVCHIGSVAIGAGWFTNHPLPDVGLFLGWCGTTGIITKLSIRLFP
                     CKRIRGVGTFVLEHEDLVPEAVYKLTHTQMAEDIVAFSSAIPPFASELQQITVNIAAD
                     SEKELEFKQEILWDDTLGEYIHRGEGAYLGFVRHLQRPQISKTSDYKKGGGFEYVGSI
                     VPIQRYPECYRKGREISERHGIPYTVTGRCVGAGHSMMFAWTFAFNRADPETIIQARK
                     ALHETDDLVLQLGGVIWKPGTYGQKLILERLDPGTKELMGKIKRLIDPNGIMNPGNWE
                     VA"
     gene            33002..34180
                     /locus_tag="PK125_00165"
     CDS             33002..34180
                     /locus_tag="PK125_00165"
                     /inference="COORDINATES: protein motif:HMM:NF014774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HPB36553.1"
                     /translation="MSLADFKYADTIHRCFRCGYCKFPVNWKDVNNCPPYARFRMESY
                     SCGGRLWLTRAWLNDQIKWTEHLAEILYSCTTCKNCVIKCPLRFNVDIVNMVIAARGE
                     MVESGRLPQAVKKFLENIELYGNPYGESRAKRGEWANRMGVEKYHGQEYLLYVGCTGS
                     YDTRAQKAARALAEIMMRAGVSFGVLGPEETCDGNEVYKLGESGLFEVLAENNIQKYK
                     NTGAQKIVTFSPHAYNAMKNYYHEYGGSFEVFHYTQFLHGLIENGKINNLRCPNMKIT
                     FHDPCFLGRWNEEYEAPRAILRAVEGVEFTEMGKNKESALCCGGGGGNFQIDLLGGSE
                     NSPARRRAREAYETGANVLAVACPKCLVMLNDAVESEELANRLEVLDISEIVIKGLNI
                     "
     gene            34218..34679
                     /locus_tag="PK125_00170"
     CDS             34218..34679
                     /locus_tag="PK125_00170"
                     /inference="COORDINATES: protein motif:HMM:NF012481.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HPB36554.1"
                     /translation="MINVLIIYHTQSGNTEKLARAVAQGVNDTENAYATFKNASNATA
                     QDVRGCNAFVICSPEYFGYMAGAIKDFFDRTYEELKDDQRIWKKPYCIVVSAGNDGSF
                     AHSHIERICKGYRFKKIQHPIICKGPVTEEMVRKCFDLGMTIAEGINAKIF"
     gene            complement(34680..35153)
                     /locus_tag="PK125_00175"
     CDS             complement(34680..35153)
                     /locus_tag="PK125_00175"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF015048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HPB36555.1"
                     /translation="MVEEKSFQDYYPPDLSHCYGCGNLNEMGLHIKSYWDGEESVAYL
                     TPEPYHIATPGFVYGGLIASFIDCHGVGTAAAAMSRSEGRTMDKEPRPRFVTASLKVD
                     YINPTPLGVQLMARGKVKEIGKRKVSVLVEVLAKGSICARGEVIAVRIPESMKQR"
     gene            35227..36006
                     /locus_tag="PK125_00180"
     CDS             35227..36006
                     /locus_tag="PK125_00180"
                     /inference="COORDINATES: protein motif:HMM:NF012596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HPB36556.1"
                     /translation="MEYENILVKTGDDHVGEIVLNRPKQWNAFNTAMAGELERALWEF
                     DRSKNVRVILLKAAGKAFCVGIDVSEFSGKTVMEYRNWIGHMGKAMITMSRICKPVIA
                     SAHGMVTAIGAGLVAASDLAIVSEDAKFGLTAINVGLNCIGPAVPVARSVGRKRTLEL
                     LFFGDLIGAEKALEFGFVNRVVPVDQLEQKSREWAVKLAQKSPLALQMAKKAFYLAAD
                     MDYYKAFECMTDNLARLCTTEDCQEGINAFMEKRQPVWKER"
     gene            36187..36438
                     /locus_tag="PK125_00185"
     CDS             36187..36438
                     /locus_tag="PK125_00185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB36557.1"
                     /translation="MKANTAGSMKSAPPNHDICRKIIALVAMIFASAKRFSRHMYLGD
                     KDILTQEFSLKRSPATVATLIWMSNNLKTIMVAEAGLVL"
     gene            36490..36765
                     /locus_tag="PK125_00190"
     CDS             36490..36765
                     /locus_tag="PK125_00190"
                     /inference="COORDINATES: protein motif:HMM:NF013677.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HPB36558.1"
                     /translation="MEKSKQKPRWIFTPQQKFKIIKDIKRCTSIQEVLTKHGISNIMF
                     YQWKRLLAMGVNTSFAQVRKTYKQCFNRMILRHHKTGSDPQSWQLSR"
     gene            37178..37387
                     /locus_tag="PK125_00195"
     CDS             37178..37387
                     /locus_tag="PK125_00195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB36559.1"
                     /translation="MKGGNLFNFCDLLCKYATTAKEEGVDGSGSCRTFVALYCKKKKS
                     LVHKNMPCIEKRMKTREKLAVKRKK"
     gene            complement(37422..37805)
                     /locus_tag="PK125_00200"
     CDS             complement(37422..37805)
                     /locus_tag="PK125_00200"
                     /inference="COORDINATES: protein motif:HMM:NF016050.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclophilin-like fold protein"
                     /protein_id="HPB36560.1"
                     /translation="MPTMIKISISEVTVYGELSDTLCGQAVANVLPIDARANVWGDEF
                     YFTIPLTMSLDETSTTSIKVGDIGFWPPGNALAIFFGPTPMSTGSDPVPASEVNLVGK
                     IIGDATVLRKAKGTSRIRIEKTMKR"
     gene            38106..38582
                     /locus_tag="PK125_00205"
     CDS             38106..38582
                     /locus_tag="PK125_00205"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HPB36561.1"
                     /translation="METKRILVVDDDELIRKFLLDFFVDLKFEVVTAENGTDALRKFA
                     PDSFDIVISDLMMPDMTGIELLKELMIKDKNILFFLITGYPTLETAVEAIKNGAYDYI
                     VKPFNLEDLRIKVERAIMTRQLKSSLKNVSGILWALIISVPIWIILGIILGFVWKH"
     gene            38595..39542
                     /locus_tag="PK125_00210"
     CDS             38595..39542
                     /locus_tag="PK125_00210"
                     /inference="COORDINATES: protein motif:HMM:NF036772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HPB36562.1"
                     /translation="MDILKPNKLVPGDTIAVVSPASPSLTPNHMERGIEAFQQMGYRV
                     VTSQHVHYRHLLFAGDEKARVSDIHAAFQDKSVQAIICARGGCGTAQILPHIDFSLIA
                     KNPKIFVGYSDITALQTAIFNATGLVSFYGPMVASDFGKSHNRYKIKNFFKILTETQQ
                     TIELKNPPSKKMITLYPGGAEGRLAGGCLSVVAASLGTRYEIETEGRILFFEDTDEQP
                     HRIDRYLTHLIQAGKLQKAKGIIFGPFPKCEYLARDNYFKFKVKLLDLIKERIVPLGI
                     PSIYGLQFGHVTNKLTIPFGGYASLDATSCRVFVEPCVV"
     gene            complement(39683..40606)
                     /locus_tag="PK125_00215"
     CDS             complement(39683..40606)
                     /locus_tag="PK125_00215"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HPB36563.1"
                     /translation="MIKLLVVGTVAYDSIQTPFGKVENVLGGSALHFTNSASFFTDVG
                     VVATVGRDFDLSAINFLKDRHVNLSGIKIDDGKTFRWEGKYGYDLNQAITLKTELNVI
                     ESFKPNIPEEMKKANYLFLANIDPALQLHVIDQVESPEAVVLDTMNFWIGNKFHELMK
                     VIERVHMLVINEAELREISKEYNLVRGARKIMELGPSCVIAKRGEYGVLMVSKDEVFL
                     CPAFPLEDVFDPTGAGDTFAGGFMGYLANMGDTSLETVKQAIIMGSLMASFSVEDFGI
                     NRIKALTYDEIKARYKSFRKCMEFGDITTLA"
     gene            complement(40603..41928)
                     /gene="ahcY"
                     /locus_tag="PK125_00220"
     CDS             complement(40603..41928)
                     /gene="ahcY"
                     /locus_tag="PK125_00220"
                     /inference="COORDINATES: protein motif:HMM:NF017071.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HPB36564.1"
                     /translation="MDYDVKDIKLADQGLEKIEWAERRMPVLRKIRERFAKEKPLKGV
                     KIACCLHVTTETANLGRTLKEGGAEVAICASNPLSTQDDVAAAIVKYFGIPTYSIKGE
                     NEDQYYSHILKALAFMPNITMDDGADLVGSLHMIALKRYEALHPKILKWVKMLGEKKT
                     KDFINKVVGGTEETTTGVIRLKSMEKEGVLCFPIVAVNDAYTKHMFDNRYGTGQSTID
                     GILRATNLLFAGANFVVAGYGWCGKGIAMRARGLSAHVIVTEVDPLRALEARMDGFNV
                     MDMKRAAKIGDIFISATGDINVIRGEHFKLMKDGAIVGNAGHFNVEIDIKSLETMKRK
                     KRKIRGQMDEYTMADGRKIYLLGEGRLVNLASAEGHPSEVMDMSFANQALCSEYMWKN
                     GRKLQKMVYSVPKEIDENVSFLKLQCLGIKIDKLTAEQKKYLASWEMGT"
     gene            complement(42084..43247)
                     /gene="metK"
                     /locus_tag="PK125_00225"
     CDS             complement(42084..43247)
                     /gene="metK"
                     /locus_tag="PK125_00225"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017370549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HPB36565.1"
                     /translation="MGMTKFLFTSESVAEGHPDKVADQISDAVLDALIAQDKRSRVAC
                     ETYVTTGLAFVGGEITTSAYANIPEIVRNTVKEIGYNDSAMGFDWETCAVLTSIDEQS
                     PDISMGVTETNSHEQGAGDQGLMVGYACDETPELMPMPIMFAHKLVKRLADVRKNETL
                     KFLRPDGKSQVTIEYIDSMPVRVDAVVIAAQHNPDVTNETIREGVIEEVIKKIIPAEM
                     MDEKTRFFVNSTGRFVVGGPKGDCGMTGRKIIVDTYGGMASHGGGAFSGKDPSKVDRS
                     GSYMARYVAKNLVAAGLARRLEIQIAYTIGVARPVSTMINAFGTSKIPPDKIAHIVNE
                     LFDLRPRPIIEKLDLLNPIYKQTAVGGHFGRNEPGFTWEKLDMVDAIRKMAGI"
     gene            complement(43357..45144)
                     /gene="ptsP"
                     /locus_tag="PK125_00230"
     CDS             complement(43357..45144)
                     /gene="ptsP"
                     /locus_tag="PK125_00230"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HPB36566.1"
                     /translation="MREEGAFTDKTLKGIGVSSGITSGRVYLLERGRVAFVKRKIREE
                     TIDKEVVKFKYAINSAIDELNAIKIAVTDDEIRKHAFIIDAHMLILQDTLFHDEVIDT
                     IRAEKINAEWALDIVVSRFLSGFEKVDDPYLRERGQDIKFIYNRLIRIMIKGKESAIE
                     MQGIRGKVIIVAHDLSPADTIQLNLNRISGFTTDVGGRTSHTSIVARALEIPAVVGAG
                     NITSLVKNNDNIIIDGDNGIVIINPSRKVQKDYIVKQLHLKAQKKEFLKIARLRAETR
                     DGFRLKVGANIELLAEMDLIKRYGAVGIGLYRTEYLYLSRKTLPTETDHYHVYRKLAE
                     NKSLQYVTVRTLDIGGDKFASQIEVPREINPAMGLRAIRLCVREIGLFKAQLMGILRA
                     SAYGPLRILIPMISGIDEVRKAKEIVDECKEELRHNKIEFNNNIKVGVMIEVPSACMI
                     SDILAKEVDFFSIGTNDLIQYSLAIDRINEYVSYLYEPLHPAVLRLIKQTVDNAHANK
                     IEVGLCGEMAGEPLYAPILIGMGIDELSMNAYAIPRVKKVIRGISQDYCKELLNELMI
                     KNSAKEGEYILKKEMGRLFPNDFSQEHDE"
     gene            complement(45141..45407)
                     /locus_tag="PK125_00235"
     CDS             complement(45141..45407)
                     /locus_tag="PK125_00235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010331997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HPB36567.1"
                     /translation="MVEGIFTIRNRLGLHARPAAIFVKTASGFESKVWMEKDGMRVNA
                     KSIMGLLTLACPLGSKIILQVEGADENQAFQELGKLIEAGFQEE"
     gene            complement(45414..45824)
                     /locus_tag="PK125_00240"
     CDS             complement(45414..45824)
                     /locus_tag="PK125_00240"
                     /inference="COORDINATES: protein motif:HMM:NF015567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HPB36568.1"
                     /translation="MIGIMVVAHFNLAQEMAAAVELIVGKQEQFEAVGIFPEEDIEKI
                     RGKIIQALKMVDSGDGVLILTDMFGGTPSNISLSFLEEGKIELVSGVNLPMLIKLASY
                     REGKTLDELARFITDYGQKNIYLAQDVLKTRKKD"
     gene            complement(45835..46680)
                     /gene="rapZ"
                     /locus_tag="PK125_00245"
     CDS             complement(45835..46680)
                     /gene="rapZ"
                     /locus_tag="PK125_00245"
                     /inference="COORDINATES: protein motif:HMM:NF003828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HPB36569.1"
                     /translation="MKTVILSGISGSGKTTFLRALEDAGFFCVDNFPLVLLEKFPELL
                     QLTGNKISKCALVVDIREKDFFEDWKHLLIDAKVKHGVEIIFLESSDESVIKRFKETR
                     RSHPILGATNVRDALKEERRLLGWIKEIADNIIDTSHLTLHELRRFVLNTYGESDVKM
                     RINLMSFGSAYGLPMEADMVLDVRFLPNPYFVDELREKTGLSHEVKEFIRSQVEYTEY
                     FDMLSKFVLYLIPLYEKEGKSYLTISIGCTGGKHRSVMVVDSLAEHLRENGYNVSTMH
                     RDVDR"
     gene            complement(46677..47618)
                     /gene="hprK"
                     /locus_tag="PK125_00250"
     CDS             complement(46677..47618)
                     /gene="hprK"
                     /locus_tag="PK125_00250"
                     /inference="COORDINATES: protein motif:HMM:TIGR00679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr(Ser) kinase/phosphatase"
                     /protein_id="HPB36570.1"
                     /translation="MKGITVQELINDIVYGLELEVIAGFKGLPRKIYNPRIQKLGLVI
                     TGHMVYLHPHRVQIFGNSEITYLNSLPEEEKKHIVSVLCTHDVVCFVVTRNLLVPEPL
                     IAEAEDKGVPLLRSKLVTSVFIERVTRLLEEKLAPSTTVHGVLMDIMGVGVLIVGRPG
                     IGKSESALELITRGHRLVVDDVVNIKKKGAVDLIGEAPVMIKNLLEIRGIGIVDIAHL
                     FGVSAVRDTKKVNLVVELVDWNEKMEYERVGLKEERYRILGIDLPLVKIPLSSGRNTA
                     AIIELASRNHVLKQQGIFTAAELEDKILKSMEGKFKP"
     gene            complement(47679..48140)
                     /locus_tag="PK125_00255"
     CDS             complement(47679..48140)
                     /locus_tag="PK125_00255"
                     /inference="COORDINATES: protein motif:HMM:NF012579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HPB36571.1"
                     /translation="MTIEEVLHESCVIADLQGEAKKDVLIELVSALKKANLIKNEQEV
                     VNVIFEREKLGSTGIGDGVAIPHGKLKSIGKIICAFGRSIKGVDFDSIDQKPAHIFFL
                     LLAPENSASLHLKMLSRISKLLRDPSFRKRLMELDNAHDIYRSVVEEDRRI"
     gene            complement(48137..48661)
                     /gene="raiA"
                     /locus_tag="PK125_00260"
     CDS             complement(48137..48661)
                     /gene="raiA"
                     /locus_tag="PK125_00260"
                     /inference="COORDINATES: protein motif:HMM:TIGR00741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HPB36572.1"
                     /translation="MGTTISFRHIEPDDDIRMYVEEKMARLHKYVETPLDVHVVLSLE
                     KKYRKRVDVTFTLNGTVINAHEVMDDMRAAVDKIIDKLERRLTRHRDKLRKHREVKPK
                     YASSFQEEGSAKTIITRTIDAKPMDPEEAIMQLEASDNSFMIFRDREKGNICIVYKRK
                     DGNFSLIETTGKNA"
     gene            complement(48817..50199)
                     /gene="rpoN"
                     /locus_tag="PK125_00265"
     CDS             complement(48817..50199)
                     /gene="rpoN"
                     /locus_tag="PK125_00265"
                     /inference="COORDINATES: protein motif:HMM:TIGR02395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HPB36573.1"
                     /translation="MLELKQTQKLSPVLTQQLQQAIKLLQLSRLELVEAIELEVQENP
                     ILELIDKEPKEDSQEEVTREETVNEKDDVTEWLERYSPSEEYAYHEERERPDYENMVR
                     KTHNLRDHLRLQAGLAELNHNERVIAEWIIENIDDNGYLAYPLSEISGTSGYSEEVLE
                     QVLKKIQKLDPPGVGARDLRECILIQYEARGEKDPIFEDIIRSFFDLFQKTNLKELVK
                     RTGYPVEKIKDVFEKVKGYDPKPGRNFADEYITYVVPDVFVVKTEDGLEIFLNDDEIP
                     DLRMNRYYVDLYLNKGTNSTTKNYIKKKITQAEWFIKSIQQRQKTLYSVAKSIVTFQE
                     EFFEKGLRFLKPLILKDVAQDINVHESTVSRITTNKYMSTPHGIFELKFFFPTGINKE
                     EGRPLSTNVVMDLICEMVKEEDKKNPLTDDEIVSLLKGRHSIKIARRTVAKYRDALNI
                     SSSRERGLLD"
     gene            complement(50186..50929)
                     /gene="lptB"
                     /locus_tag="PK125_00270"
     CDS             complement(50186..50929)
                     /gene="lptB"
                     /locus_tag="PK125_00270"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004091254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HPB36574.1"
                     /translation="MNGTSKLVAQTLSKSYSARKVVDNITMGLNVGEIVGLFGPNGAG
                     KTTTFYMIIGFIKPNGGKILLDDEDITTLPMFLRARKGITYLPQEPSVFKKMTVADNL
                     MSILEFHHVEREMMGHRVLELLESFKLEHLSNNYADSLSGGERRRLEIARALMTAPRF
                     MLLDEPFSGIDPISVSDLKKIISGLRKKGIGILLSDHNVRDSLPICDRAYVVNNGKVL
                     LEGTPEAVANDKIVREVYLGEEFYIDVGA"
     gene            complement(50951..51841)
                     /gene="lptC"
                     /locus_tag="PK125_00275"
     CDS             complement(50951..51841)
                     /gene="lptC"
                     /locus_tag="PK125_00275"
                     /inference="COORDINATES: protein motif:HMM:TIGR04409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HPB36575.1"
                     /translation="MNSRTAILYIASGFVAASIIFAGYLFFKKPEKIAPPSIEEAKSV
                     IVFKDVKYSGEKKGVIEWEIRGKSGKKHVDKPVVEMEEIDGEYKPKSDLFVRFKGSKG
                     VMDTEQETGTVEDVRIIHNNDYALTTKYLNFDFKKGLAVTDAPVKIEGSRLTLMGIGL
                     LSNAKEETIKLMKNVTGFIEAKNGKYRFSADSFMYSMRDNRYLLDGNALMSGQDLRVT
                     CDRLYIYTDGEEIEKVDARGKVRMYAKGSIAKNEQSVYHFKGGKVGPTASSLGEKNHS
                     GTAGKTNPKKPAGGKALAGQ"
     gene            complement(51941..52909)
                     /locus_tag="PK125_00280"
     CDS             complement(51941..52909)
                     /locus_tag="PK125_00280"
                     /inference="COORDINATES: protein motif:HMM:TIGR00393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KpsF/GutQ family sugar-phosphate isomerase"
                     /protein_id="HPB36576.1"
                     /translation="MSRFIDIGKEVLRKEAEAILSVMSCLNVDFERAVDAIAQCRGRV
                     IMMGIGKSGLICKKIASTLSSVGVPAIFVHPTDSIHGDLGILQSGDVVIVASNSGETE
                     ELIKVLPWIRRMNIIMVVITGDPESTIAKYGDIVISVKVDEACPHNVVPTSSTTATLA
                     LGDALAIALMDKRNFSIENFASLHPGGTLGRKLVLTVEELMHTGDQLPRVFQDAPMKD
                     VIFEISSKRLGVAAVMNRDGEIVGVVTDGDLRRAIERFNDVLSKNASDVMTKNPKAIK
                     KDALATYALKKMEEFSITSLFVVEKVGERRPVGIIHIHDLLKAKIA"
     gene            complement(52906..53718)
                     /gene="kdsA"
                     /locus_tag="PK125_00285"
     CDS             complement(52906..53718)
                     /gene="kdsA"
                     /locus_tag="PK125_00285"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012364209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008676 - 3-deoxy-8-phosphooctulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HPB36577.1"
                     /translation="MQKEVKVSAVTVGAKPFVFIGGPCVIEGSDITLRIAERLKDITS
                     RLAVPFIFKSSYDKANRTSISSFRGPGIDEGLKVLEAVKNNFQVPILTDVHSVEEAKT
                     AATVVDVLQVPALLSRQTDLLVACGRTGKPVNIKKGQFLAPHDIQHAINKVESTGNFK
                     IMVTERGTYFGYNNLVNDFRAIPIMKRFSYPIIFDATHSVQKPGGMVTVSGGEREFVF
                     PLAKAAIAVGVDAIFMEVHFDPSKALCDGANSVCLDDLPLMLETLKRIEAAL"
     gene            complement(53703..55358)
                     /locus_tag="PK125_00290"
     CDS             complement(53703..55358)
                     /locus_tag="PK125_00290"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: protein motif:HMM:NF003792.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HPB36578.1"
                     /translation="MRQKFIFVTGGVVSSLGKGLASASIGALLESRGLTVAIKKLDPY
                     INVDPGTMNPFQHGEVYVTDDGTETDLDLGHYERFTNSVGSKKSNFTTGQVYDAVIMR
                     ERKGGYLGGTVQVIPHITNEIKKRILDVNDGADVAIVEIGGTVGDIESLPFLEAIRQL
                     RNDLGKENSLFIHLTLVPFIKSADEMKTKPTQHSVKELRGIGIQPDILLCRTDRLLSQ
                     DLKDKIALFCNVDKDAVITGKDVDVIYECPILFHREGLDEKITQLLNIWAKKPDLSKW
                     EAVVDTIRNPEKYVDIAIVGKYVKLQDSYKSLHEALVHGGIANNARVNIHYVDSEDLE
                     KGGDLTIQFQGVHGILVPGGFGDRGIEGKMKAIQYARENKVPYLGLCLGMQLAVIEIG
                     RNLAGLKGAHSTEFDTATSSPLIFLIDEWVNQKNKVQKRDRFSDKGATMRLGAYPCKL
                     KAGSLAFKAYNEKLIYERHRHRYEFNMAFRKKLEACGMNVTGISPDGQFAEIIEVKNH
                     PWFLACQFHPEFRSKPFSPHPLFRDFIKASLKGKQERQRCRKK"
     gene            complement(55361..56545)
                     /locus_tag="PK125_00295"
     CDS             complement(55361..56545)
                     /locus_tag="PK125_00295"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00306.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cofactor-independent phosphoglycerate mutase"
                     /protein_id="HPB36579.1"
                     /translation="MKYIVIIGDGMADWPIERLDGKTPLMAAHKPYMDMMAQDGYCGK
                     VLTIPDGYPSGSDVACMSLFGYNPAKYYTGRAPIEASGMGIHLDKNDVAFRCNLVYLA
                     IHPDKTFMGDYSSGHITTEEANILIQDLNKGLGSKEFIFFPGVSYRHVMIWKDGMWEM
                     ETTPPHDITGKEILDYLPKGKGADRLISLMIDSRRILEGHSLNRKREKASRYPANSIW
                     LWGQGKKAQFPLFKDNHGLTGATVTAVDLVKGISRLAGFDTPRVEGATGYLDTNYRGK
                     ANKAIELLSDHDIVYIHIEAPDEASHEGNIDEKIKAIENIDREVVGPIFESVGDFVKF
                     LIVTDHATPIAMKTHHATPVPFTVFQKHNRIGGCPSGYDERAGERLFDGEALIKFFIT
                     GH"
     gene            complement(56691..57980)
                     /locus_tag="PK125_00300"
     CDS             complement(56691..57980)
                     /locus_tag="PK125_00300"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF004976.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HPB36580.1"
                     /translation="MIGVGIIGLGTVGIGTYKILEQYHSLIKDKSGQDIRVVKCADID
                     TKRPRDISLKQGIMTAEAREVIECKDVDIVVELIGGIEPANEYIRMAIQNKKWVVTAN
                     KALLAEKGDELFTLAAKQNVEIGFEASVCGGIPLIKAIRDGLVGNRINYILGILNGTS
                     NYILTKMTLEGMSFEKALEEAQRLGFAEADPTFDVEGIDAAHKLCILIRLAFQCHVKM
                     RDVVTGGITKIDPIDIEFAREFGYRIKLLAVAKEENEKIEARVEPAMIPVVHPMSNVD
                     GVYNAVYIVGDKTGPTLYYGKGAGSEPTGSAVVSDIVDMAYRMRGGSPLPKIPSVSKA
                     KKMRSGEKSFVPYYMRFTAKDRPGVLSKVSGVLAEYNISISSVIQKGRKENGYVPIVI
                     LTHEATEQNLKNAKAAIDRLAVIKGESVSIRIEEGRL"
     gene            complement(58306..58788)
                     /locus_tag="PK125_00305"
     CDS             complement(58306..58788)
                     /locus_tag="PK125_00305"
                     /inference="COORDINATES: protein motif:HMM:NF015941.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine-specific kinase"
                     /protein_id="HPB36581.1"
                     /translation="MDIKTVEINVPEGLNIIIGQSHFVKTVEDLYEILVGSSPSLKFG
                     IAFSEASGPCLTRYEGNDEELMRLASETSFSIGCGHTFVVFLKEGFPINVLNAIKVCQ
                     EVCHVICATANPLQLIVAESEQGGGILGVIDGFRPKAIEKEEDKKARKELLRKFRYKL
                     "
     gene            complement(58860..59786)
                     /locus_tag="PK125_00310"
     CDS             complement(58860..59786)
                     /locus_tag="PK125_00310"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HPB36582.1"
                     /translation="MKDAGKTLENCRLCPRACGVNRIRGNVGYCGVGKEILVAHFGPH
                     FGEEPPISGTRGSGNVFFVSCNLRCVYCQNFQISHECKGKSVTLEGLVEIFFSLKDMG
                     VHNINLVSPTPYVPFIAEAIECAKSKGMQIPFVYNSHGYETADALKMLDGLIDIYLPD
                     FKYWNSNIAEQLSFASNYPESAKKAILQMKQQVGNLIVKEGIARKGTLVRHLVLPSNL
                     AGSNRILLWIKEELGVDTFISLMAQYNPMHKATEYPMLARSIRQEEYDFLLNFLEEEG
                     FNNVFIQDLESVKTYVPDFELEEPFLKQELRS"
     gene            complement(59933..61039)
                     /locus_tag="PK125_00315"
     CDS             complement(59933..61039)
                     /locus_tag="PK125_00315"
                     /inference="COORDINATES: protein motif:HMM:NF015445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HPB36583.1"
                     /translation="MMDVLWKIMIDKEEEIAYKEKKPRRGYLMKGRLMVKCFLLLFVV
                     SICFVAFPGLSAAQQKTITLNYSNFFPAPHKNSQLAEAWCKEIEKRTDGKVKITYFPG
                     GTLTPAAQTYENVTKGIADIGFSVFGYTRGRFPMISIMDNPLGYKSGVQATKLINEFY
                     KKFKPKELDDVHVLYLHAHGPGILHTKKAVNKLEDLKGMKIRAQGTVVPIVQALGGAP
                     VGTTMPETYDALRTGVVDGAMSPQEALEGWKWGEIVKFTTQNFGSAYTATMFVVMNKN
                     KWNSLPPDIQKIFNEVSAEYADKQGKLWDEIDKLGYDWSATKGHKVIALSKEEDAKWA
                     AAVKPIYEKYAAEVQAKGLPGNEALKFCLDFLKK"
     gene            complement(61407..62714)
                     /locus_tag="PK125_00320"
     CDS             complement(61407..62714)
                     /locus_tag="PK125_00320"
                     /inference="COORDINATES: protein motif:HMM:NF018513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HPB36584.1"
                     /translation="MNEVVVGIIGLCIVLLLFLTGIELAFAMILIGFLGFGYLISFHA
                     ALNLLAKDVFDVFNSYSFTVIPLFVLMGQIAFNSGIAKRLFDAAYKFIGHVPGGLAIA
                     TVAGATAFKSICGSSPATAATFASVAVPEMDRYSYDKRLSTGIVAVVGTLGVLLPPSV
                     TLIIFGIITDQSIGRLFLAGIVPGLISAFFFVVIIYGWCKMNPALGPKGPKSSWAVRI
                     KSVPEVAWVILIFVLVIAGILKGFFTPTEAGSVGTFLVLILVFAKRDLNFKGYWKSAL
                     ESVRTACMVLFLIAGSTVLGHFIVVTKIPMIAADWIVSLPLPSWLVLVFIALIYLLGG
                     SFIDDLAFMILATPIFYPAIQKLGYDPMWFAIMICITVMAGVVVPPVAINVFVVKNIT
                     KTPFSVIYKGVYPFLLSLLFVAILLFIFPALATWLPGRLMGAM"
     gene            complement(62707..63189)
                     /locus_tag="PK125_00325"
     CDS             complement(62707..63189)
                     /locus_tag="PK125_00325"
                     /inference="COORDINATES: protein motif:HMM:NF016200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HPB36585.1"
                     /translation="MGGFLNVVLRLSNWMHAIAGAALTFIMLLTVSDVFLRLFARPIV
                     GTFELVGLGGAVIIGFGIPITSWNRGHIFVDFLVNMFPDSLRDGFNIVTRLISMGLFI
                     LVGWNLFVLGIDMVKSGEVSTTLLMPFYPVAYGLGVCCFIQCLVLICDIVKIAGGKYE
                     "
     gene            63456..63683
                     /locus_tag="PK125_00330"
     CDS             63456..63683
                     /locus_tag="PK125_00330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB36586.1"
                     /translation="MSFEYAMLPNRKEGIPHNVGASSALYYVTIPPHINLEARHEVFG
                     KQGITSPRNGSSDSSLNTKENLSLVQNPGNR"
     gene            63658..64119
                     /gene="rimI"
                     /locus_tag="PK125_00335"
     CDS             63658..64119
                     /gene="rimI"
                     /locus_tag="PK125_00335"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: protein motif:HMM:TIGR01575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HPB36587.1"
                     /translation="MFKTQATDETIAKMTLDDLNGVMEIEKLSFISPWTERFFEEALA
                     SPVSLSLVLRNSTQPIGYIILYSVADEAHILNIAIHPGFRGKGCASSLIEHVIGCCKK
                     RGVHQFFLEVREGNVAAINLYRRFGFEKIGKRKKYYAETNEDALIMCLSVR"
BASE COUNT        17379 a        14961 c        13526 g        18252 t
ORIGIN      
        1 aggaagactg caatcccagc aataacttta gcagtgtcat gcattatatg tccctgtact
       61 tttcaactgt aagccatctt tccaaatcat tctgcttcca gaggttctcc gcgaagattg
      121 gttgtccgtt ttttctctcc tgtcatgatg agggcattcc ccacaagttc gtggacacca
      181 cacacctcag tgcccggaac ccagtaatcg aacagaggag gcagagaagc cttatctatc
      241 gcacacatac aggctactat attaacacca tatttttcct tcacatactt cactgcgttt
      301 gcccttggga accctccccg gagccgcatt tccatattct cgtcagcgcc aagccctgcc
      361 ccactgccac aacaaaatgt tctttcccgt atcgtattct ccggcatctc ataaaaatga
      421 ttacaggtgt ttgtgataat gtagcgtggc tcctcaaaga gacccatgct tctagcagga
      481 ttacaagagt catgaaatgt taccttccac ttatcattgc ggcttttgtt gagattcaat
      541 tttctatggt aaataagatc cgctgtaaat tcggttatgt gaaccatctt tgttgaacgg
      601 gcattgccga aagtagtgcc ggttatagga gacaccggtt cttcgaggga atctgcaggt
      661 ccattaaagg tgtccatata ctgatggata gtcctccaca tatggccaca ttccccaccg
      721 agaatccatt ttaccttgag cctcctggcc tcctcataaa tcttggcatt taatcgtttc
      781 gcaagttcgg atgaatggaa aagcccaaag tttccccctt ccgaagcaaa agcgctccag
      841 gtatagtcga gaccaatctc atgaaagagc ataagatagc cgaggaatgt gaaccagtgg
      901 ggactggcaa aatagtctgc agatggggca acaaaaagaa tttcagcacc tttccggttg
      961 ataggcgctt caatggcaat acccgtgagt tctctcaact catcaagggc aaactcaaag
     1021 gtttgttgaa aagcattggg cggaacaccc aaatggttgc cggtcctgaa acagttggat
     1081 gccggctcta caatccactg gatatttagt ccgaggagat tgagaagttc tcgtcccatc
     1141 atagtaattt cacacgtgtc gataccgtat gggcagtaga cagaacacct cctgcattcg
     1201 gtgcactggt agaaatagta gaaccattcc ttgagaacgt cttcggtgag atccctggct
     1261 cctgcgaacc gcccaaagat cttgccgcaa aacgtgaagt atctcctgta gatggaacgc
     1321 agaagttctg ccctggcaac aggcatgttt ttaggatcac ccgagccaac gaagaaatgg
     1381 catttgtcag cacaggctcc acagcgaaca catatatcga gaaaaagcct gaaggaacga
     1441 tgccgtttta aaagagtcct cattccatca agaatgattt cctgccattt atcaggaagc
     1501 ttccagtcga attcgttcgg cttccaatcc ctgacatagg gcagatcaag ataagcagca
     1561 tgttttaccg gagcactgta acagtaagtt cctttccgaa aatcaggttt tggagtaagc
     1621 aaatcccccg aaggtttcag gctaatatcg agcatctcgt gtgatgtttt ctgcctttcg
     1681 tcagccacgc cttttctccg gtgattctat ggtggacaaa gaagccggga atccgagagg
     1741 ttgttctttg tttttttcaa gaggcaaccc ggcctctcta atcgcctctc taaattcttc
     1801 ttcccattcg tcatacgtat gggtttttac agggtaactc cacgggttaa tatggcgttt
     1861 tgccctgctg tcgtttttca gatttcttgt ggggctcatg aaaactcccc ccatgtgcat
     1921 gagtttgctg aatggaaaat atgccaaaag aacgctaaca agaaaaaggt gtacataaaa
     1981 gattacacct gtttcttttg gcataacagg gtcaaaactt acaagcccaa gggcaaatcg
     2041 cttggtttca acaaggtcaa cccggtagat atgcctcata agtaatccag acactgcaat
     2101 tccaaggatg agcaggagcg gaaaataatc agaaatgagc gaaatatatc ggatctgtgg
     2161 gttcaagatt ctcctcaaga acaggtagga tagtgctata ataatgaggg catccgttac
     2221 aaaaaaggtg ggggtaaata cctggaaaaa accatcaagg ttctgaagaa aagtaacatg
     2281 cctcggaacc ggctcaaaaa acagtctcaa gtgcctgagc aggatgacga ggagagacca
     2341 gtgaaagata atccccccaa accacagtaa cttgttaccc caataggttg cctgttctcg
     2401 gtcggtcagt ttagtacgat catttcgaaa aagagacctg aaaaagataa tttcaagcgc
     2461 catacgccag aagattcccc aagtggttga agggctttca gttttgttgc ttcttatcca
     2521 gggaagcgat ttttgttgtc cacatacagt cggtatgtgg aagggaacag gggagcacgc
     2581 ccagaggatg acgcggtaca taaagccaat aataaaggca accagggcag catatggaag
     2641 tactatggca aatagcgcat ttaaaacgtt caacctatca cctcgaactt accataagtt
     2701 tgttgctaca ctgtcagaga atctattatc aatgtattca aagttctcat tactgtcaaa
     2761 atagtataaa agcttaactc tgtgttgaca aggtcaaaaa tgctctgcca atatataatt
     2821 caaacccgaa atcccacttg tttgatcaat ctttggccat tgaaatctct gcaggttcat
     2881 gagttatggc gtctcttgtg ccacgaaact gacatgaaac aaagaatatt gttccccaag
     2941 tggcttgatt tatagccctg caaaattaaa aggtcacaac gtggaaaaaa attactacac
     3001 tcactcagat tagaatagtc gcacgtttgc ataaaaatca ttcacgagcc ataatacagt
     3061 tgcgcgtact ttataagaac aacgaaaatg actgtgtttg taaccgggcg cctgacacct
     3121 tgcttttgag caagatcctg ctcatgttct accggctatc tgagaaacga tggattaata
     3181 tctaccgtgg cccaattcga ggattcggtg gagttcaatc agggcaaatc agaggcaatc
     3241 tcaatccacc tcagagggct tagaggtgat taagggcttt gtatctatgc aaacaccttc
     3301 tgttgagcat gactttttct gcgtcccgga tgtcagggat taaatgagac agtgtggaat
     3361 aaagcaatca aagactgatt tgaaccctat ttagagattg ttttttcaag ggtatcccgg
     3421 atcaagcaac agcccctgtc ctgtttccgt ctggacacag agaggttcat cgggctttat
     3481 cttcggtgaa acacaacgca atttatgtcg tggccacatc aagaatgtag attaagtatc
     3541 gaaatttcaa ttatggggaa cctttttccc gaaatgtttg cgattcaaga catataccag
     3601 ccacattgtc ccaagctgaa ttgcaatcgc cagggcagag atcacccaat agccaacgga
     3661 aaatctgttt atcagtccag cgtcaaccag cccgcggttg agccagaact ggatcatcac
     3721 ggccagtgcc actcctgggc aaaccaacgc atacgatccc accgatgcct cttttccaaa
     3781 aatataccgt tgtgcatagc caagacgccg caatacagcc catgttaaga gtgcgaataa
     3841 gagctgtgct gaaagaaact gcgacaacat catgagccgg ctggcgctgc cgctagcctg
     3901 cgcaaagtgt tctccaaggc cgtggctctg gcgcaggaca agaatgccgg tgactgtcag
     3961 tagagggata aaaatcgata gagttggagc tgtttctata tcagcaccgt tttccagtaa
     4021 agagcgaatc ccaagaacaa gtgcaatgac acctatcagc atagcagcaa ccagaaagaa
     4081 cgtagaaagc accagcccga tgcctgacac taagggagca gctgacattc cagccggtgc
     4141 ggccaggcct acgccgatca tagacaaggc aaaagcagga agtgcctgag caaaggagtt
     4201 attagcaatg caactgaacc cacctgttgt caacgcgcgc ccaagaaatt tccctaacat
     4261 atcgaaggcc agaatgctga tagcaacaaa agcaattatt gccaagggaa agaggtattc
     4321 tacaacgctc cacaaccgtg gtacaaatac catcccaagc ataaagcaaa cattgacact
     4381 catagcaagg gccaggggca tggccattag ctgggtttcg gcattggagg acctgatttt
     4441 gatataagcc tctgttcttt tccatttttt gaattggatc aggttccaga tcaaaacttt
     4501 gatattgagg aaagaataga aggcgatacc agctactgcc attataatca tggcttttgt
     4561 agctaaactt cctgaattaa aagcagttgc gatatcttca aataccggta cagaacgacc
     4621 tggatgactg gtccagtgca tcaaccacat aaaaaaggtt acgaccagac cacctgaacc
     4681 aagtgaagcg agaaaataca acggggcata tgtttcagaa ggttttgtgt gcccggtttg
     4741 catgactacc tccttatttt tgtgtggaca ggcccaacac aacgattaat tttcactatt
     4801 atggtcatat ctttagtcag agcgcaaaca ataataagct tccccctcac aatcttagga
     4861 gaagaagctc attcatttca ttcatattct ggcaagacca acagtcacaa attctttgtc
     4921 aaacaaatca tttgttgggc ttttgaaatg atagtgtctc gcctccttcg gatctgcacc
     4981 acagatactc cgctccaatt tctcaccata cttttttgat tactttgccc acagtctttg
     5041 ctttttcgct gccataatcc atcacgatga cctctggacc ctgtagatgg gttttttccg
     5101 ttatttgcag aactaaaacc atttcttttt tagattaatg cttactaatt taagtatatc
     5161 agtctttgtt caattttgga aaaatatgcc ctgcaagcct gaatatacgt gctcgaaatg
     5221 gtattcttca tgaccaacaa atcaggcccg caatcgatct ttttttatca cccgatttca
     5281 tttcgagctt tctttggtcc ttgtatgtgt tacaaagcta taagactgca tccnnnnnnn
     5341 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5401 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnncatttcg agctttcttt ggtccttgta
     5461 tgtgttacaa agctataaga ctgcatccag ctctgactgc aagaaacctg ggttttaata
     5521 ccaggaatat agactcgaaa ggctgattct ataacatgag gggttcatca aggaggggat
     5581 aggtgaagaa tggttttcat gccagatgat acgttcgttc atattagtca ttttacgtgc
     5641 aaatcaatat catggctgcc aaacctaaaa caatgccaaa tccctgtttc aatgaaatgg
     5701 tttcattcag gaaaattact gataatgtaa cagtaataac aggggagaga gctgtcaata
     5761 ttgcaattaa tgaaactggc ccctttgaaa cggcgtagag gtaacacagg gctcccaaaa
     5821 accccagacc acctgtggtc attgccagca atacaccctt gaggtgaata tcgggtttaa
     5881 acttcaatgt gaataaaacc attgtagcaa cgagaacacc gcccatcact tcaaaaagta
     5941 ttgcacttct agggctaata tatcttgttg ttatcttagg gataaagctc cagaatcccc
     6001 acagaacaaa tgcaccaaag gtaggtaaca gccagtgttt catagaagtc tccttattta
     6061 taatcaatac agaatcgcca gtactttcca agataatcta agtctatcat ccatcgttca
     6121 gcaggacaat gtttaaggca ggcaaactct tcatttcaga aacaggttgg cgttcttcga
     6181 tgaatgtatg attggcattg cccgtctgct tgcccacaaa ccagacgctt ttatatttca
     6241 ccgggcaatg atggcataat ctacatatta tgcatctcct atccatgaca gcaatggatc
     6301 ttttagggaa attgtatctt tttgaagttg tccctgaaaa tcaattttga atagataaaa
     6361 gacagtactg ttgagcagga ggtcgaaaac cgattaccca ggcttttatc ctgcctcaca
     6421 gctcaggaac ggggaggatg ttctatttct tccttaatcc acacattttt ggtattggct
     6481 gtatagacat gggggtcatt cagcactgat cagccataaa tctccacaga tttgctcgct
     6541 gacatgacag tcatttctct tttgccaaac aatatattat tgtacccgct gcaaaaacaa
     6601 aaaaatatga tattttttct ttatccttga gaatgattga atttttctta gagttttcct
     6661 tttcgttact tttccccttt tggtatcctg actcttgcaa gcctgttttt aatggttatt
     6721 gcagagtcct tatccttcat cttgatgcgg ttgatgtctt cacgacattt gtcataaatt
     6781 ttgcaagcca aagaagcaag gtcatctatc cttgtctcga aaagagctaa atcttcaagt
     6841 atttcaggtt tagtcctttt gattgcctct tcatcattta tcttcagctc ttctcttacc
     6901 gcctttttga ggaaataaat aaactcaaca gcctgtgacg ggctgaaatc ctgaaccgtg
     6961 cgtattctga tgatactgtc cagggcagtg attattttgc tccggtttcc ctcgcatgta
     7021 agctcatgga aaagtatctc acagcctgtt gagattgcaa agccaacagg atttgcgaat
     7081 ctatcttttt ccatttttaa aaaatttaca acatcagccg ggtacgtctc cacggtgagt
     7141 gaaatccatc tttgcaatat acggtttttt ttctcctcca gcgtagcatt caggttcata
     7201 gcctgtactc aaaacggtgg tttcggatcc gtatacaata atccagatct ctcatcatca
     7261 ctcttgatca acagtcatga atcaggggat gtcctcgttg gatttttgga aaaaactgct
     7321 ttttacatct ttaaaacagg ctgcaacctt ttcaccatta taaatcataa tgcctggccc
     7381 cttaagaggt aaacgtataa gatgtaccgg cagtattcag tatgaactgc tctggcattt
     7441 ccctggcaaa gcgcacaatt gcctcaaaac ctgtacaatg agtaggtaca atgtagtcag
     7501 gggaaaaacc ttttatatct tcaatgtaac ggtctataag gttggtctgt gcattgacca
     7561 gatggaatcc accaatgatg gcgtgtattt ttgatatgcc gctgatttga accacatact
     7621 tgacaatatt tataatgcct atgtgagcgc agccgctgag gataacaagg cctctgtttt
     7681 tgacaacaaa aaacagggca agttcccctt caaacaaatc ctgctccagc cgatcacctc
     7741 tcttgacaag taaatttgga ttacctttct catagtctgt cactttttcc acagggcctg
     7801 tggcgtaacc gcccggtatt atttccctgg gttccgtcaa ttcccttata tcgaccactc
     7861 caaacttatc tatctcagct ctttccagtt ttcctatatc catgggctca ttgtctgatg
     7921 gacgaataga aaaccggcgt gcaaaggttt cctgaccgat gtaaagcgga gtgccttttg
     7981 ggatacgatc cccataccgt ctgagaaact ctgtgagccc accccagtgg tcaaaatgac
     8041 cgtgactcaa agccatggca ttgaccctgc caacatcaat gccaagaacc tctatattgt
     8101 gagtcatgcc catgaggtcc atgccgtaat caaagaaaag ggtcgatgaa accccgttca
     8161 cctctgtctc aatatggtac gaaaggccgt gttcagcatg tataacagac cccggccttg
     8221 atcggaacac tgttgagact tttgtgtcag gtctcagggc atcataataa ttatcagtaa
     8281 taacagttac taccaggcga tctacttctg ttgcagtaat gggctgcctt gtcatatgtg
     8341 taggacctcc tgaaaagcca ggatttgcct ggctaccttg ctaatgatcg cacgtatttg
     8401 caccggtctg caggacgcct ttgaggtatt gttccacaat ttccttcgga tcttctccct
     8461 gtgcgccggt aataacattc acaccctgtt gtgcaaatag ttgctgcgcg cggcttccca
     8521 tgcctccggc gataatgcag tttacgccct gctgagaaag ccagggtggg agaagacctg
     8581 gttcgtgagg cggaggagta acgtaggtct cattgatcac cttgccagtt gcatctgtat
     8641 caatgagtgc aaaggcctca caatgaccaa aatgggcaca taattttccc tgatgtgttg
     8701 gtattgcaaa tttcattgaa gcctccttgt ttaatgtgga tatgttttct ttcgtatctt
     8761 tgtctcccgg ctgcgttgca gtgggggttt tattctcaga ttcctttttg attcccagcg
     8821 agttgtcttt tgcaaagaag attattcttt caattatgtc ttcgaatacc tttgctgttg
     8881 cagatttgga gtagaaatag atataaggtt tttcctcatc tccgctttgg gcaatggccg
     8941 ggtcgagggg aatgcttcca aggaatggaa tgccgtatcg gtctgcaaga agctctcctc
     9001 caccctttga aaaaatctca gtggactgac cacagtgggg acagacaaat ccgctcatgt
     9061 tttctatcac tccagctaca gggagttcaa gttgtttaca gaaggtgata catttttcaa
     9121 catcgatggt ggcgacctgt tgtggagtgg tgacaattac tgctttcgcc ttttctttga
     9181 gaaactgagc tactgagagg gtttcatcgc cagtacctgg aggacagtca acaacaagat
     9241 aatcaagatc accccattga acgcgggaaa tgaaatcctt tatcatacta tatttgaggg
     9301 gccctctcca gattacagcc tgttcagtcc cctgtagcag gaaacccatg gacattacct
     9361 tgagaagatc cgactcgtat gttataggga caatctcctc tcccttcatt tctggccatc
     9421 tgttcatcaa atgtaaaagt tttggaacac ttggtccatg aatatctacg tccataagac
     9481 cggttttgtg tccccgtaat gcaaggctca atgcaaggtt tactgcgaca gtgctcttgc
     9541 caacaccccc tttaccggaa aggacaacga taacctgatc tattttactc agtttgtcgg
     9601 ccatggcaag ttcgtccagc agggggtttc tttgattgtc ttgttcgcat gtactcataa
     9661 ttcctccaaa atctttgata tttttttgtg gatcgttctg ataatttcac tcgtttcact
     9721 gtcgggtgct atatccagga ttgtttttgc ctgtatctgg gcttcagtta tcctgtgatc
     9781 gtaaggaatt gccccaagaa aggcagtttc ctttgtaatg caataatctt caatgcgttt
     9841 gcaaaaatca ggatttatgt cgctcttatt tacaattaca ccggttttac atttgaagaa
     9901 ccgtgcaagt tcaatgatac gctccaggtc gtgaaacccc gaaatggtcg gttccgtgac
     9961 aactgtcgct acatgtgttc ctgtcatgga agctataacc gggcagccta tacctggagg
    10021 gccatctatt acaataaggg ggcaaccgtt tttttcagcc agagcttttg cctcactacg
    10081 gaccatggta acaagtttgc ctgaattctc ttctccaggc agaagttccg catacacgta
    10141 aggttgagag tatttatctt tgcatatata acacgaaccg gcgacagtgg agggaaaatc
    10201 aatagcattg gcagggcaaa gaaaataaca agcgccacag ccctcacact gacgatgatc
    10261 aacgatatag tcagcagaaa ttgcctggaa cctgcagaca tccctgcatg taccacaact
    10321 ggtgcaccga gacggatcaa tggttgcctt tttaccgcca atgaattcct tttcttcaat
    10381 gacgacggga ttaagaatca tgtgaaggtt tgccgcatca acatcacagt ctgctgccac
    10441 cctgtttgtc atgagactga ccagagggcc cacaacagag gtttttcctg tacctccttt
    10501 accgcttacc acaatcactt cttttatgtc agtcatgttt atacacctat cacagatttt
    10561 ccaaatgctt ttcctatatt gattgcgaga tcactgaaaa ggggtctgag ttccggcaga
    10621 gcctcaataa taatttctcc cctggagtaa tgctcggcaa ttcttctgtc ttcaggaatc
    10681 cttgcaagaa tttccattcc atgatcgtca atatactttt ccaggggctg aaactgttcc
    10741 ctttcacgat taataactag ccccaacgga atttcaagct tctcagccac tgcaactgct
    10801 atagagaggt catgaagacc gaatggggta ggttctgtaa ccatgacaac gatatctgcg
    10861 tcactgatgg ttgcaacaag cggacaggcg gtaccagggg gtgcatcaat tatgtgaaca
    10921 cgggactcca aactcccttc cttgacctgc ctgatcaagg gcactgccat tacctctcca
    10981 acgttgagta tcccatgtat gcaggcaatt ccttcaatgc cagtgccttc ctcgataaac
    11041 cctacttctc tttttgcctc aatgatagca ttttcaggac atatatgaac acaaagaccg
    11101 cacccatggc acaatccagg gaatacaagg gggggaatat caggaaggct tacgagggca
    11161 ttgtattgac acttagaact gcattctccg cagaaggtgc atttctcctc aatgattttg
    11221 ggaaccataa catacgcagt ttttcgagat tcaatctttg gcttaagaaa aagatgacca
    11281 tttggctctt caacatcaca gtcaatatac tggacagact gcccggaggt ggccaggaag
    11341 tatgcaagac tgactgcaat cgtggtttta cctgtaccac cttttccact ggcgatggcg
    11401 atattcatag ggctgtcctt ctacgctctc gtcattggaa ttccacattt tggacatttc
    11461 tgttcaaaac agggaatccc tctttggtga ggcgaccgct gcccacactg tgggcaaaca
    11521 cattcaccgt ccggccccag tcctcttcca cccattcttc ctcttcctcc ccttccaggc
    11581 cccagacgtt ggcctgctcc tgttccgcct cccaaaccct gtccaccacc ttgtcctgaa
    11641 ccctctcttc tgttatccgc cattgttggc ctcccttctt ttgctggggc ttgtatcttg
    11701 cccctctgtt tgcaaagccg tcagagattc cttttcatgt acgctctgct cattcacaga
    11761 ttcccgcaga aaacgcaaac cctgcagggt atcgcgcttt caagggtaga tgaattcggt
    11821 ttaccagtgg gacgatacat cagcactggc aagcttttcc agtttccctt cctcaaacct
    11881 cttgattgca tcagcgacaa tcatatcagt tgcagaataa acttccagcc ctgccacctg
    11941 cactacctga aatgcttttg gcccgaaatg accgctgata attaccttcg ctcctgattt
    12001 tactacgttt tgtgcagcct gtatccctgc tccctgtgcc gcattgagat tcatggagtt
    12061 atctaacacc acatgttcct ttgtttcagt gtcgtaaaca ataatctttc tggctcgtcc
    12121 gaaacgttca tccagctttc cttccattgt accgtctaca ctcgttatgg ctattttcat
    12181 ttctcatcct ccctcaggct tataatttct tttattgatt tctccggtaa tgattttgcc
    12241 cggtaagccc atctcttttc ccactgccaa aagccgggca aaatgtcact tgcagtcaga
    12301 ttgccatcct ttattcttaa gtccatacca ccttccaaag catttgttat tttttggtgg
    12361 gcagtccagg cagatttgaa atgggttacg ctcgggaaaa aaccagcttt tcctggactt
    12421 gtctgtctgg tcacttataa aatccctcct tgcctttgca tcaactacta tatgaatata
    12481 tattcataat gaatatatgt caagaatatt tccataattt tgttcccgta gtagtaaagt
    12541 cgcttaagga tgtagcctgt atgaaacagg ggttaggtgg ctaccatttt ttctgctgcg
    12601 gacataacag cttctataat ctttttgtat ccagtgcagc gacaaatatt tgaggaaatg
    12661 gtgcgaataa tctggtcttt tgaaggcttg ctttgcctat ccagaaggct tttagcagta
    12721 agtatcatac cagaagtgca gaaaccacat tgcactgcgc ctttctccat aaaggcctgc
    12781 tgcaaaggat gaagtatacc attcttctcc agaccttcca ctgttgtaat actttttcct
    12841 tcaacttcag gcgaaagaac aaggcatgat ctcaccggga tgccgtcaag aattaccgtg
    12901 catgccccac atgaaccgat gctgcacgat tcttttactc ctgtcaggca gagtctctcc
    12961 cgcagggttt ccagaagggt ttcatattgc tttacttcta ccgaaacctt ttctccattt
    13021 acagtaaaat taaccttgac ccttttcatc agcagccctc ccctgaaagt tttctaacga
    13081 ttcttttcac aagaacatac accatttttt tgcgatacgt tggagaaagt actgtgtttg
    13141 ctacaggcgt tacgtgactc gctgcctccc gggcaatacg ctctccccat ccatcttcgg
    13201 tctcatcgaa gtcgaaatga agtggttttg cgccgattcc accgattgcc agacacccgt
    13261 tcccgttctt taacgatgct gctgctccga caagagggta gtcaatcgag tccctgactc
    13321 tgatcttgag gtaacaaccc cttgttttat ttgcggggat aaaaatgtgt gttaatattt
    13381 cgccaggctg caaggtaaaa ggacgatctg actttccact gaagatggct gccaagggaa
    13441 tctgacgact gccatcaacc cctgacaggc gtacctgtgt ctccagggtg atcagggccg
    13501 gggcattatc acttgtatag ttggcaaaac acatttttgc cccttttact gcattgcata
    13561 cctgcccgcc cattttcagg caatatcctt ttgctttcct ccactcatcg gacttgttat
    13621 aaaaaagaca acgagtattc tggaggacat tgccgccaat agtacccctc atctgtattg
    13681 tctcgcaagc agtggcctca agggaatcag caagggctgg cagattgtct tttacgaaag
    13741 gatggttttt taactcatat atggtaacgt ttgcaccaat cttcagccag gaccgggaca
    13801 attctatctt attcaggctt gagatatttt ttaagtcgat tacaatagag ggcttctcaa
    13861 gcctctgctt gattcttggg acaaggtcgg ttcccccaga gagataaact gcatcgcctc
    13921 tgtttgcctg gaataattga agtgcttttt ttattacctt cggttttttg tattcgaatt
    13981 ctggcagaat catttgtccc ctcccttctt ctcgatcatt tccaaaatcc tgtcaggcgt
    14041 aagaggcagt tcactgaact gaacaccaag agcgttgctc acagcacatg caacagcgct
    14101 atatgcgttc attcttacag taagcccccc ctcctttgct ccaaaggggc cttccggatc
    14161 attggattca acaataattg tctctatgtc aggcatgtcg catgccagag gcactttata
    14221 ctcgagaagg tccgggttta gcaggaaacc ctcatcccac agatttcctt cggtaattgc
    14281 agcaactccg gcctgctgaa ccgatccttc catctgtcct tctaccgcca tgggattaat
    14341 ggccttgccg caatcgtggg ctgtgacaaa atgcggcatc gttattttgc ccgtttcctc
    14401 atcaatttct acctctgcaa cagaagtccc aaatgaatag gtcccagcca tttggccaaa
    14461 aggcttagaa atccattctc tatcaaaatc aattttaggg atataagacc caatgcctgc
    14521 tattggtttt gccctgaaga aagcctctct tgttatccat gaaataggca tcttcttttc
    14581 agggtttttt cgacagataa ccattttgtt ttgaagatca aggtcttcaa cagcctcgtt
    14641 gagtttttct gaagcaaggg caaggatttg gttccgagca tgctcacagg ctttttttac
    14701 cgcgttgccc cctgcaaatg tagctgcctg tgaatatgct cccggatcaa ggtttgtcac
    14761 ctctgtatct gcacttacca ggttgatatc ttccatggga aggccgagaa tctcggaagc
    14821 aatttggact gccatagagt cattgccctg gccgttgtca acaatacctg taacaaccgt
    14881 tgcatgacca tcatcgttca gtttaacata gcaggacgag ctcgacctga aacccatagg
    14941 gaaaccagtc attattgcca cacaacccat accaatacct ttatttttcg ggagctttcc
    15001 ccgtttactg ctccaccctg ctttctctgc agccttttgt gtcggttctg caagattacc
    15061 gcttgtgatc cacgattttg ttgctgtctt ttctcctaaa gtcaggccat tttttatccg
    15121 tatatccatg gggtcaatac caagatcttt ggcaatcata tcgagttgca cctcgttgcc
    15181 ggcaagaaaa gcccttccgt gacacccata catgccgcga atgcccttgt ttgtgtacac
    15241 ccggtatcca tcatatttta catttggaaa acgataggct tcctcatata catagtacgg
    15301 aatcgatgcg gcaatgggcc cagtgctgct atacgcccca ccattgtata tggctttgaa
    15361 ttctttggcc aggattgtgc cgtcctgttt gacgccagtc ttgatggtgg caacaatgtc
    15421 gtgtacctgt ctgctgctta tagcgttttc ttctctcgat aggacaagtt tcacaggttt
    15481 tccggacttc atcgacagga gcgccgttat aaaatctcct ggtgcaacct ctgaacgacc
    15541 gccgaaggca ccacctgaaa agatgtgccg taccctcacc ctgttgagtg gaatctgcaa
    15601 gaggttcgaa agggcttttg ctcttacatg tggacctgca ttgggcatcc aaaaatcaag
    15661 gtagcctgaa ttgtaatggg caacaaccgc atatggctcc atcatggcat atgcttcacc
    15721 tgcagctcta aatgtgtctt ctcgaacaat gaatgactcg cggaatgctg attccacatc
    15781 acccacatca atatggacat ggacattgat attgcctgga tatgcatcat ggatctgagg
    15841 ggcatccggt gccatagcag ccacaggatc gaatactgcc ggaaggggtt cgtactctac
    15901 ctttatggca tgaagagcct cgagtgctgt ttcttcatca attgctgcca cggcagccac
    15961 atcttcaccc acataccgta ccttatcagt gggcaacata tcatggtcct gtgtgtaacg
    16021 gaaaacaccc cattttactc catacccgtc ttgtccgctc acggctgctt taactccagg
    16081 aattttcagc gctggactta catcaatagc caggattcgc gcgtgagcat aaggactcct
    16141 cagcaccttg gccacgagca ttcctggcaa cttcaggtct gcagaataac aggcttgccc
    16201 cgtcaccttg gcaagatagt cagacctaac gaccctttga ccgataacct tgtgattttc
    16261 catgagcccc ccttgtgtta tcgttgccct cagcccaatt ttttcggaga gggtcaaatt
    16321 tttctgttta atggtgattt caaatcagaa ttctatcacc ctccatataa gaggtgcaag
    16381 aaatttccga gatacaagat tactgcgaac agcaactccc tggatttcca cacgagcccg
    16441 gtgcaccaca acacccgcca gatacaggta cagaaggtgt agaagaggac attgaaaagt
    16501 ttgaagccga catcagttta taaagatctg tgctctcgca atttttgcat ctcagttcgt
    16561 ctgtgtcact actccgaaca aaaaactcat tctttgcacc acatttcatg cattggtatt
    16621 cataaatagg catgttactc ctccatatat aggatcaaga aagtctgtta aaacctgcta
    16681 taaggttttt tagctccggc atttgcctaa tgatgtccac aaccgctacc actttggtga
    16741 ccactgcacc cgtcattaac agtaagttct gtaagttttt tttgttcgaa gaggtcaagg
    16801 ttctcacgga cagaccctgg agctgaccta aacactttaa cgcctgcagc attgagtttc
    16861 ataagtgccc ctgccccaat tcctcccaca acaaccgcat ctatcggcag gccgcctatt
    16921 gaacgtaatg ggttacaggc cccgtgagcg tgtccagcat cttcgttttt cactgacgtt
    16981 gcaactttct gattcgtgtc gaccataatg aacaacggtg ctgaaccgaa gtgtccgtat
    17041 acgctactgt tgaccccatc atctttttcg acagcaaaac aaatcttcat ccggttctcc
    17101 tcgaagccaa tattttcggt ggtcagcatc tttgtttctc atcgaaacga aaaaagctgc
    17161 catttcgccc aaaaatctgt gcggcgtttg caggaaactc aacgacattt ttttgggcac
    17221 cacatgatta tataataagc atatggcaga aataagttca agattctgtc agtataatat
    17281 gtctgattca agattgtgtg ttatccttag acatccacgc tcagtgatga tacgagaaaa
    17341 actacaatgc atacagaaaa gccaagcaag cacagtaaca cctttggaaa attttttatg
    17401 atggaaaaag cgaacaattg tgtggtcaaa tcgattagcg cagaatgtgt gacgcttgtc
    17461 ttaaagtctg tctaacatac cttgttgccg gagattcatt atttttcccg aaagattgtt
    17521 tgggaacagc acataaccct cttggcacga acgaaatcaa tcaggtaact gattatttca
    17581 aaattgattg tttcattaaa caagatcgag gtgaaaaaac tcataggccc gtgacctttt
    17641 gtacgttcaa ccttaatttt tttcataaaa ccactatcct cgacaggaag gttacttatg
    17701 taatgaaaat cgctttggga gcccatgaaa gacgagcctt aaggcaattt tttatcttct
    17761 ctaactttga ttgcagtact atctagcgtg aactggggtg ttaaaaaata ataatcttac
    17821 actttccctc ttctttttaa agtgtcagaa tcactgatgg gctccacata tgttttgttg
    17881 ccctcataca cagaaatcac attcaagtct gcaaagtgat tttttatatc cattaattct
    17941 atctcagaaa gcggagggac cgggcaggac ctcaaaggcg tatttatctg tatttcatca
    18001 ggggcaatct tacgagataa tgatgctatc ttccccgcgt attttttgtt ctcctctgta
    18061 aacataattt ggagcgcaaa ttttccatta taattcttcc taaacttgag gagtcccttt
    18121 accacgtttt caaaatgaat catatcagat ggcttgttga cggcctcaaa agtctcggct
    18181 gaaaatgcat cgagtttcgc gataacaaaa tccgccaaag aaagatcttc ttgcacgctt
    18241 gcccgatcca tggttgacga atttgttatg atagcgattg ggtcagggcg caatgctttc
    18301 acagctttga cggctatccc cagattggac gccagagttg gctcaccgcg gcctgaaaag
    18361 gttatgaagt ctatagggat ttttgggggt aacgatctta actctctcag tatgtcatca
    18421 acgtgaacat aagcgatgcg ttggttcgaa taagtttttg ttgcaccgag ctggcagtaa
    18481 atacagtcaa aagtgcagat ttttgatttc tgtgagagca ggtcaatccc taaggaacta
    18541 cccagcctcc atgaaggcac tggtccatag acatatttgt atgtattttg tttgattttg
    18601 atcatgttat tgttcgaccc ttttttgaca tattatgctg ctaccggccc aaggtgatct
    18661 tcatttacag agaccaatga tatggtgcat agtcgttgac ctgcccttca agcaacaaag
    18721 gtaaaccttg aaccagctgt gctaagtaca agctgttccg gcatctcctt gtaaaacagg
    18781 gtcatagcat cataaccagt acaatgcatc ggaattatgt attgtggttt cacagactta
    18841 atatcttgca cagttctttt gatattctct gtgcctaaat caaaaaggtg aaaacctccc
    18901 atgaccgcat gaacatcgga gataccggta attttctgtg cgtgtctgac cgtattcacg
    18961 attcctgcgt gtgcacaccc ggacagaatg atgagtccct tgtttctcaa gccaactatc
    19021 atggcctgtt cacccctaaa atcatccatt tcaagacgct cattgcgtgc aataagaagg
    19081 gtggttcttg ctttttcata ttcagtgacc cgatctatat acccggtaaa gtaccctccc
    19141 ggcataactt ctgttggcag ctttatttct tttattttta gattttggat ggatgagatt
    19201 tgcttaacgt ctaattgtcc taaatagtgt ggtgaacccc agggatatcc cgaaaatctg
    19261 ggggcaaaag cctcttcacc aacataaagg gtggtctcct ccgagcttgc tcgacggtat
    19321 gctttcaatg tctgtaagag accaccccag tggtcgaggt gcccatggga aaggccaaag
    19381 gcgtttactg tggcaaggct tacattaaga atggccaagt tttttattat tgtttcaaag
    19441 tttaagccga aatcaaagag gaaggtattt ttttcaccat ccatcgttgt ttcaaccaca
    19501 taggagagac catgctcagt atgaatcggg acaccacggg ctgaagatac cctctctact
    19561 atgggcgaat cattttgaac agtgaagtaa tgattatcca caactacggt aatcctcagt
    19621 tcatctacct gtaccatatc aacaagttca gctaccaagt taacctcatt tacccatcct
    19681 tcttttctca agttctgtga tctgattctt tatctgctct atctgttgat ttaatgcttc
    19741 agcctgttgt tttagcatat ccagctcttc atcagtgctt gcaccgcctt ctccccgatt
    19801 caacacttga ggaccggggt aacttccctg attaaacagg gcgtctgtgc ccatgcccct
    19861 tcccattccc atgccccttc ccattcccat gcccatacct aagccccttc ccatgcccat
    19921 gcctcttccc atgcccatac ctaagccccg agagaaggca gggctaaaat attggtcccc
    19981 tcctgaaggc gcacctgcac cggcatgagc gggtacgtta ggttgtgttg tgtttgccag
    20041 atttccttgt ttaaattgct gaatgatttc ccgaataaca cccgaacatc caacaacgat
    20101 attgattctt gcagcattga gggtttggta ggcatttggc ccgcaattac cggtaaggac
    20161 atgggtaaca cccttgtcag ccattaattt ggctgactgt atcccggctc ctccaacaag
    20221 ggagatactt ggattctcaa cagcctcaaa gtctagagta tccgtttcca caataaggta
    20281 ataacggcat cgtccaaacc gtggttcgat aacatcgtcg agagaggttg agtttgatgt
    20341 aactgctatt ttcatgattt ctcctatttt ttctgcaaca gccacggcaa cccggctgcc
    20401 gagaatttat ccggcaaccg ggttgccgtg tagtatgcga tgatcagagg tggcattatt
    20461 cctttttgtc tttagcctcg atctcttcta tgcgctgccg tattcctgca atggcttcct
    20521 ccaaataccc ggcttgctct ctcagcgcat tcaattcctg ctcgtcgctc atgataggtc
    20581 ctgcacctgc cctataggga aaaccctgaa accagggatt accccacact ggcaatccaa
    20641 aacctgctcg ctgccagccg gtaaaacccg ttgcaaagaa tcgattgcgc caacctcgtc
    20701 ctccaccgcg tccacctgca aaaccccaaa aagctctacc agggccaata ttcattgatc
    20761 ctggcactcc aaatccagta cagtaacctg caccgcgtcc tgtcattgga cccattcctg
    20821 ttggacccgt tccatctcca aaaggcatag catacctcct tcactgaatt aatctgtttt
    20881 tggtgacttt tgtattctgt aaactaccaa tttcaagact tgccatatac cacatcaaaa
    20941 ctatagtttc gcaacgattc gtctccatat ctcatatcac cagaaccatc gtctcccacg
    21001 tccctggcct cttccacatc tccatccaac agccccaaaa aaatagggtt gccggactcc
    21061 aaagggcatc catcggcgat atgcaggcct ttgcataggc accacattgc ctctgtaatt
    21121 ccaaagaggc ctccttggga ataggtttgt aaacccggga gcattgttgc ccgcgcagta
    21181 accggctgca cgtcctgtca ttggacccat tcctgttgga cccgttccat ctccacgagg
    21241 catttttctc acctcctttc actatctcaa ccgctctgga tgttgctatc gtcttccacg
    21301 acgaccacgg aagcggtgtc tttgccgctg tctgtaacat ccaggcataa agaagccacc
    21361 atcatcgagg tgaccctgga gaaaagcagg aatcacagcg cccagatcac cacaaaggaa
    21421 ccctatgact tgaacgccgg ttgccataat ggctttttcc aatacatgtg aaatagctcc
    21481 acagagaagc acatttgtcc ccagtcctgc tacctctttt gccctgccaa atggatcacg
    21541 catttttaga acaacgtttt cccttgacac ttcacgctga ccctcaatct ggatcaggca
    21601 aagcctgtct gaaacatcga aaacagggga tatacggtct tgccaatgag cgatggctac
    21661 tttcatatct aatagataag cacattcgat gccataccct ctctcatagg ttaaacatga
    21721 ttaagaacaa tatgttagct ttgtttacca tagattgaca cacatttgaa tcttgcagat
    21781 tgcaataaat ataaattatt gtattgcaaa ttacattatt atgatctatt atatctatgg
    21841 aatgcaaacg gaacttttga ctgataaaaa cgcacgtgat gttattctcg attctatcaa
    21901 tgaaggtgta tttaccgttg gacttgattg gcgaatcacc tcttttaacc gggctgcaga
    21961 gcatattaca ggagtatcga ggaatgatgc aatcgggaga gcctgttcgg atgtgttcca
    22021 tgcagatata tgcgaaaagg attgtgccct taggcgaacg ttcgttacag gtgagcccac
    22081 tgtaaacgcc actgcgcata ttatcagcaa ccaggggcac agagttccca tacgtatttc
    22141 tgctgcgatt ctcaaggata gtgacggaaa tgctattggt ggcgttgaaa catttcagga
    22201 tctaagtcag gttgaacaac ttcagaaaga gctaaaaaac cgtcacacct tcgaagatat
    22261 cgtgggtcgg agcccgacca tgatcaagtt gttcgagatc ctcccgcaga ttgccgaaag
    22321 cagtagcaca gtccttattg aaggcccgag cggtactgga aaggaactat ttgcccgggc
    22381 tattcacaat ttgtcaccta ggaagaaaaa acgttttgtt cctgttaatt ccgctgccct
    22441 tccggacacc cttctggaaa gcgaactctt cggccataaa gcaggtgcat ttacagacgc
    22501 gaagcgggat aaggcaggtc gctttgtatt ggctcatggt ggaacaatat ttcttgatga
    22561 gattgccgat atttctcctg cactccaggt tcgactcctg agggtccttc aggaacgtgt
    22621 tgtagaacct ttgggaagca tagaaccgat caaggttgat gtacggatcg tggctgctac
    22681 aaataaaaat cttgcccaac ttgttcaagc aggtaaattc agggaagatc tttactaccg
    22741 tattcgtgtg gtccacctta cattgcccgc actcaatcaa cgtcgcgaag atattccttt
    22801 gctggtagat catatggtgg cgaagtttaa tcgtcttcaa aacaaagata ttgctgggat
    22861 ttcccgcgat gcaatggccc acctgatgga atatgactac cccggtaatg tacgggaact
    22921 tgaaaatatc attgagcaag cctttgtgct ctgcagggga ggtatgatcg aactgcatca
    22981 cctgccacca gaacttcgcc cggttgcgaa tgtgacatcg aagggctttg gccccatgag
    23041 tatggatgca atggaaaaac accttattat cgaaacgtta caacgtcatg gcgggaacag
    23101 gaccgaggca gcacaagacc tcggaatcga tatgagcaca ctgtaccgaa agatgaagcg
    23161 ttttaaaatt gatgtgcctg gcagagatgg tcggggtaag agaagacagt aatgcatttt
    23221 gcacttatat cttatggaac agattacaat gcagcctttt tcaatatgta gccatgttaa
    23281 tctaccttgt aatgtcaatg tgttatcatt taatgaatga gtttgaacat ggctggcaca
    23341 gctcttgctc tgccagcctt ataggaattc gaagcaagag tggtagagtg ccttaaggtt
    23401 taaaggaata tgaagacatc cctcgattgc ataccatgtt ttttgaggca atcattggag
    23461 gcggcacgtt ttgtctcaga tgatcacgcc tttcatgaat atattcttcg taaagttcta
    23521 aaaattatga tagacattga tcttatgcaa tctccagccg tggtagctca atggattcat
    23581 cgtcaaattc gggaaataac tggccaagaa gacccatacc gtaatgcaaa agagcgtttc
    23641 accgaggccg cactcatttt gttacctgaa ttttctgctc aggtggaagc ttctgcggac
    23701 cccattgagg cagcagttcg tattgcaata gcaggaaaca tgatagacat gggcccagaa
    23761 aatgtgctaa cacctgaagg tgtgcggcaa acagttttgc aagcattttc tgaacccttg
    23821 tacggagacc tggaaagttt caaacgcgca atagaagcag ctaagagtat actttatctc
    23881 gctgataatg caggtgaaat cgtgcttgat caggtgctca ttgagcgact tcccctggga
    23941 cagacaaccc ttgttgttcg ggggcatccg gtaattaatg atgcaaccat ggcggatata
    24001 gaggcaataa acttgaatcg ctttctgact gtcaccgaca atggttctga tgcaccggga
    24061 acgattcttg atgactgtag ctctcagttc cgtgaacttt tcaaaagcgc cgatctcatt
    24121 atcgcaaaag gtcaaggaaa ctatgagaca ctcagcgatg aacccaaggc aatctttttt
    24181 ctgttgaaag tgaagtgtaa ggttattgct tctcgagttg gattggatat gggaacccac
    24241 gctattttgc catccggctt ttgggcacag ggcacccggt cttaaaaaat tgccatagta
    24301 ccagtaggaa gttagaccaa caaaaaatat atctaatttt gaaggaggtt gcggttatga
    24361 gtaagaagtt gcaaaacatg gacgacattg tacgcgatgc acgtatgaag gaggaggctg
    24421 aaaatataac caagaggctc agccatatac gtcacaaaat tctggttatg agtggtaaag
    24481 gcggtgtggg taaaagcagc atagcggcct atctggccgt atcccttgca ggacggggtt
    24541 accgagttgg tcttatggac gtggatcttc atggaccgag cattcccggg ttgttgggcc
    24601 tgaaggacaa gcttctcacc accggtggac ctgaaggaaa aaccttccct ttgaaatacc
    24661 ttccaaagtt ggaggtcatg tcaatcgaag ttcttttagg agacaaggat gctgcaacta
    24721 tctggagagg acctttaaaa ggtggtgtaa tacgccagtt cattgcagaa attgaatgga
    24781 tggaattgga ctacctcatc atagactcac cgcctgggac tggtgatgaa cccttaactg
    24841 ttgcccagac cattccggac gcaaaggcgc tcattgttac gacacctcag gaaatttcac
    24901 tggctgatgt gagaaagtca ataaattttt gtaggaaaat gaacttggaa gttctcggtc
    24961 ttgtagaaaa catgagcggg ctgaaatgcc ctcactgcgg gaaaacaata gacctgttta
    25021 aaaatatggg tggcatgaca actgccaaaa aggaaaattt gaggcttctc gggtcccttc
    25081 ctatagaacc taaagttgtc gagtgcggag atgaagggag tatagatttc ctgggggatg
    25141 acagcctcga gtacaaccgg gcttttagtc aaattgttga tacgattgta aaagtaatgg
    25201 aaaataagga gtaaacagcg tagtcagatt gatattaaac gcttaagctt gattgaaaag
    25261 actaataaac ccaatgaaat gattatgcga atatctaatg tacggcaatt tccaaggagt
    25321 ttgtgattta tggttttacc gaataacaag gagataaaac gatgtctgga aaacttgacg
    25381 attttttaac aaatttacaa aatgagattt ttgatgatac cattaagaca tatggtgaaa
    25441 cggcatatga gagatggcgt aatcctttat atatgaaatc gatacacaat ccagatggct
    25501 atgcccgtgt aaccggaccc tgcggtgaca caatggagat ttttctgaaa ttcaatgagg
    25561 gaagggttag tgatgcgtca tttcagacgg atggttgcgg accgagccag atttgtggtt
    25621 cctttgcggc tgagcttgct tttggtaaaa acatagatga gctcaaggaa attaccaacc
    25681 agacaattct gaacattgtt ggtggcttac cagaagaaaa ccagcactgc gctttgctgg
    25741 cagcaaatac tttacatgat gccgtggata actacctgaa gaagagattg tcttaagacg
    25801 ataagatacg ataagcaatc aatacatagg taaaatcatc acccttatcg tgtacatatg
    25861 tcattctatg ggtgacagat tataaaaaac aatactgttc ccacgccaca cagaagaaga
    25921 aggaattggg ttatcccaga ccaaaaatct gtcttctgat gaagatgtgg aatcagatct
    25981 gctgttgcaa tgtagatgaa gcttgctgca gaaaatgcaa gtatgtaagg aattgcagct
    26041 tgagcacttt ttaagcaaaa atagcctatg agggagccag gcagtgtggc cattgatgaa
    26101 aataaattgt acatgaaagc cttgtttttg gaatacccgc tgtctaacaa tatggcaaag
    26161 tcccctgtct cctgaggtac ttcatgtgca attacggcaa aagaggtggt taatccgaga
    26221 ggtatgcttg tcaggaaggc tgcagcaatt acaattccat cgacaaaatt atgaaaggtg
    26281 tcgcctagaa gaatgagaga accggcagtg ctatgaactt cacagttctc aacgtggcaa
    26341 tgtcgccata tgacaagttt ttcaagcaga aaaaaagaga tcatccctac aagcactgtc
    26401 agaaggattg acgatgaaga tgcagaaacc aacgcttttg gtatcatccc caacagggaa
    26461 gaagcgagta acgtgcctgt ggcataactt atcagagaag ggattagcat aagcctgact
    26521 ttatcaggga agataagcaa caaaaatgcc cctgctaaag aaccaacgct tcccagaaaa
    26581 ctgaaaatta ttattaaaat gagcttatcc attgaatctc cctaaaggtt gatgactgtt
    26641 tatctgtctc atcacaaatc cgaaagctgt gatttaccgc tgtgttttct tatcattttc
    26701 catttgatcc tcttgtataa tatgaggagt gtgattgata cgagatagcc aatgtgcaat
    26761 caggcggcaa aataccataa tcaaattggt attagtgaaa aataatcact aaacatttga
    26821 ctttaattaa taatgaatat atattcatta aattgtattg tcaacgagtt tttacggagg
    26881 ataccttttc ttttgatgga aataaaaaag tcgaaacagt aaactctccg ttgacttaaa
    26941 gagggtgacc cgctataaat gtgtaataat agggaaacaa tggtaatgaa attcggtgtt
    27001 cgcatcgttc ctatctttca gtgtagattc gtgtctccct ggatcaagat gtctgcctta
    27061 aggtaggaaa tgttaggaaa tgagaggtct gttcaatgaa ttacacatac tgctcatctg
    27121 atatggcttc ataacagtag gtgggtttat caccgaacac atttatacaa aggaggaaat
    27181 gatggtggaa cagaaagatg gatgcgtcaa gccttcacgg ggccccatag gaatccctaa
    27241 ggcagaaact ggagatattg tccaaactaa tcaaaaggag ggtggcttta tgatagcaaa
    27301 agtcggcaaa ccggcaccgg attttgaggc gagtgcttat attgatggag gattcaagaa
    27361 tattactctc tcagaattta aagggaagtg ggttgtcctg tgcttttacc cgggtgattt
    27421 cacctttgtc tgacctacag aattgtcggc ggtcgccgtc aagcacaaag aaatagagga
    27481 actgggggtc caggtacttg caatgagcac cgatagccgg tttattcata agatatggca
    27541 ggaaaacgag ctttcgaaga tggttgaggg gggggtccct tttcccatgc tcacagacgc
    27601 cggtggcctc atcggtcggg tctatggggt ttatgatgaa gaggcaggtg ttgacattcg
    27661 gggccgtttt attatcgatc ccgatggaat catccaggcc atggaggttc tcacgccgcc
    27721 tgtaggccga aacgtgtccg agtttattcg gcagataaag gccttccaac atgttcgcgc
    27781 tacaggtgaa gccacaccat caggatggca acctggtaag gtaaccctca cgcctggccc
    27841 tgacctcgtg ggcaatgtct ggaaagtctg gaaacccgaa atggcttttg agtaggctgg
    27901 gacgaaaggt tagccaaata tattgtcctc gatttaaaca accgtatcgg taaatgaatg
    27961 agaagggcac tccttgccct tctcatttta cctttaaagt acggaccttt aggattaaag
    28021 gcagggtctg caacactagc agtgaagaac atgatgaaga atataatatg gagatgacgg
    28081 atggatcagt caacagacat tttaccactg gaaatagcaa aatcacgtct cgaagagata
    28141 atcagagaga atgatttgaa tgactcagat atatctgtac tcgtgaaaac cctatcccct
    28201 gaggaagcta taggcgttcc tggcaggagg gattttccta ttgtggaggg aagggagcgt
    28261 gtgatagaag ctgattttca agggtcaaaa gctcatgctt tcaccgattc gcccagggaa
    28321 tttattggaa aattgaagga cgtaatttca ctaccattgg cagatagcgg cgaaagagca
    28381 gtctttatag ctacaatgaa tgctgttttg aaatattgta aaattattga aaccacctta
    28441 cattgcaaag atgaggaccc ggaaaagtgt gctattgaaa tttcttccca cattcacaat
    28501 acgcttagag caaaaaaagt cggcctcatt ggcctgaatc ctgcaatcct tgaggccttg
    28561 agcaaaacct ttgggccgga aaacgtgcgt gtgactgact taaatggtaa aaatatcggg
    28621 tctgtaaaat acggcataaa tgtctgggat ggcaaaaaaa tgactgaaaa acttgtcgag
    28681 gaatctgaac tggttctcat gacagggacc accctggtaa atggttcctt tgaaaagata
    28741 tggagtaccg tacagcaata taaaagagag tatctggtat acggcgttac atccgcaggg
    28801 gtatgctcgc ttgcaggcgt gaacaggata tgcccttacg gaagaaaata gaaaaccaaa
    28861 gcgataggaa taagagattc cattctcaca atattcagga ggtttaaatg ccgtttggtt
    28921 ttggtaaagg aagaaggggt gcagacaaaa caggaggaat gagtagttct gggtcaggaa
    28981 gggggtacgg ccgatggggg catgggggtc ctcccacaga gtgtgtatgc cctcaatgtg
    29041 gccttgtagc cccacgcgaa ccgggagttc cttgttttca aagaaggtgc ccacagtgtg
    29101 gatcgtttat ggctcgtcag tttttaaaaa ttgaatagtg ccttttttct tgactatgga
    29161 acgcagcgga gaacacaggg aactactcaa acagggtcga caggttgcca ttaccgctac
    29221 catcgtaatc tttctccttg ctgttgcaaa atttgttatc ggttatcttt ttcagtcaca
    29281 aattctcatt gctgacgcct atcacagcgg cgttgatgtc ctcgcaatct ttgcgtcatg
    29341 gtttggttta cagctcgcat caaggaagaa gagcgcaaaa tttccttacg gactatacag
    29401 ggctgaaacc tttgtaacgc tcgccatagg agcttttatt acatgggcgg gcatacaaaa
    29461 tcttctggaa ggatacaaga aactttacat tcttgccact gaccaggctt ttcctctcat
    29521 gcccgttatt gttagtggag cttccgttat tgtatctttt ttggttgcaa agatggagcg
    29581 gcgagcagga gttttcatta attctggagc tctaaaggcg aatgcatcgg acgcgtttct
    29641 tgacaccggc acctccgtgg tcgttctctc aggcattctt ctggcacatg caaagattcc
    29701 ctatattgaa ggcttgatcg tgatgtttat tgcagtgctg attatcaggc ttggcataat
    29761 gaatgtatgg actccaatgc tgattctttt ggatgcaaac cttgacccga ggcttcaagt
    29821 ggcaatagag aatagaatag cgctcattga tggcgtaaaa agggttaacg acgtaaaaat
    29881 tcgccagtcc ggtcctttca ggatggtaga atgcaacata gcaactaaac catcggtttc
    29941 cgtttttaaa gcacacgaac ttgcaagtac ggtcgagaaa atcgtgacgg acgaacacag
    30001 ggagatcgag tcagtatttg ttcatataga acctgttcaa caacacacac tatcagccat
    30061 tatacctgtc aaagagatag atggaatgaa ttcaagggtc cacggccatt ttggcagggc
    30121 gccttatttt gtgatcttga aactacacga cacggggaca gcagaaatag aagacttcta
    30181 ttacaatgag ttccttgaaa aaaaagatca aatacatatt gctgttaaag tcatcaaagt
    30241 aatcatcaaa cacgacttgg atatcgtttt tacaacgaaa atcggggaga ttgctttcca
    30301 tatgctgaag gataatttta ttgacatttt tggtacggaa gaagacataa ccgtgggcga
    30361 ggtggtgaaa caatttcggg aaggccgcct tcaaatgatt gctggacccc atccagctga
    30421 ggagtctgag atacggcatg ctaaggagga cttcaagtga ttctgccgga gggaaaggtc
    30481 tccacgtttt ttaatatttg tctggcggtc aaaaaagtta tgattgaatg aaactaatat
    30541 agactaaatt gcactcactg aataaaaaag atgctcaaag gagacggttt tgtatacgct
    30601 aaaatttgat attgaaaaat gccataattg tcaaactcag gcatgtctca ccaggtgtca
    30661 acatctggat attgatcggg aagttgccca gagagaaatc cgcaagattg cacggggaga
    30721 ggattccttt attcttcatg attgcctaac gtgttatgcc tgcgaggaat actgtaccat
    30781 gggaaaccac cctttttacc tgatcgtgga acagcaggag gcattaaata tcccgcctgt
    30841 gcctcggccg ttaattcaaa gagctgtaaa cctgggtgtc ccatttcggg gtgaacctga
    30901 gattggagaa gtacacggac cggtactaaa tatggcagcc ttttctgatc tgataggttt
    30961 gatccaggga agactttttg aagggctcac cgtaatatct acggatgatc gtaaaatgtt
    31021 ccattacttt tgccagctta tgtatcttca ttatgcaaga agttcggtaa ttaaggaaag
    31081 attgccagga gttatcaata cgataaacag gcataaacca actgaggtga tttgctttca
    31141 tgacgaatgt tacggaacgt atacatcata ctgtcctgct gtaggcatag atgttccatt
    31201 cacgccgatc catttttttg agtttttgca tcgccggctc accgaattaa aagaagtaat
    31261 cagaccggtg ggtttcaagg tagcctacca gcgaccgtgt tcgtctcggc tttcaccgga
    31321 caaacaccac tttgtcaggg atatttttga acttatagga gcaaggtatg ttgaaaggga
    31381 gtatgttgat gaaaatgccc tctgctgtgc aggcgctatc cagggacaga gacgagaggg
    31441 aagtcgaaag cgggctattg aaatgcagcg gaaaaatatt aatgatatga agaaagcagg
    31501 ggccgaggta tgcattttta actgccctgc ttgttttcaa accctgggct ctttgttatc
    31561 tgccgaagac attaaaccta tgtacatgag cgatctttgc aggctagcca tcggtgaaaa
    31621 gccaatggaa tggaggtgat tctatggatt ccatttatga agcactggtc aatatagtgg
    31681 ggaaagagta tgtttccaat cagccggagg aactctacat atattccttt gatttgggga
    31741 cagcggaacc gtgtcagcca cagtacgttg tttctccgag ggcaacagag cacattcagg
    31801 gtatagtgcg tcttgcaaat aaggagaaga tacctattgt gcctctgggt gggggactgt
    31861 cccttgctgg ccttgctgtt cccctcaagg ggggaatcct cctcgatctt aagcgcatgg
    31921 acagcattat agaggtgaat gaaaatgccc gatatgcagt ggtggaatgc ggtgtttccc
    31981 agggccagct cacatcatat ctggaaaaaa attatccgcg cctcacccac tctgaaccag
    32041 gtgcgcctcc agccgcgacc cttgcaggaa atgtgatgat ccacgggcaa ggagaccttg
    32101 ctcaaccata cggcttcaac tctgatctgg tcaatggcct tgaggttgtc cttccaacag
    32161 gagaggtctg tcacattggc tcggtcgcca ttggcgcagg atggtttacg aatcatcctc
    32221 ttcctgatgt aggccttttt ctcggttggt gtggcacaac aggaataata accaaattat
    32281 caataaggct atttccgtgc aagcgtatac gaggcgttgg aacctttgtg ttggaacatg
    32341 aggaccttgt tcccgaagct gtttacaagc ttacccacac ccaaatggca gaagacattg
    32401 tagctttcag ttctgcgatt cctccctttg caagtgagct ccagcagatc acggtcaata
    32461 tagctgcaga ctctgaaaag gagcttgagt ttaagcagga aatcctttgg gatgacactc
    32521 ttggagaata tattcacaga ggagaaggtg cataccttgg ttttgtaagg cacctgcagc
    32581 gccctcagat ctctaaaaca tctgattaca aaaaaggtgg gggtttcgag tatgtgggca
    32641 gcattgtgcc catacaaagg tacccggaat gctacagaaa ggggcgtgag atttcagaaa
    32701 gacatggcat cccgtatacg gttaccggtc gttgtgttgg agcaggtcat agcatgatgt
    32761 ttgcgtggac gtttgccttt aacagggcag atccggagac tatcatacaa gcgcgaaagg
    32821 cacttcacga gacggatgat cttgttttgc aactgggtgg agttatatgg aaaccgggta
    32881 cttacgggca aaaacttatt ctggaacggc ttgatccggg cactaaggaa ctcatgggga
    32941 agataaaaag gcttattgat ccgaatggaa taatgaaccc gggcaactgg gaggtggcgt
    33001 aatgtctctt gcagatttca agtatgcaga caccatacat cgatgcttca gatgtggtta
    33061 ttgtaaattc ccggttaatt ggaaagacgt caataattgt cctccttacg cccgttttcg
    33121 tatggaaagc tattcctgcg gtgggaggct ttggcttacg agggcttggc ttaacgacca
    33181 gattaagtgg acagaacacc tggcagaaat tctttactcc tgcaccacct gtaaaaactg
    33241 tgtaatcaaa tgcccccttc ggtttaacgt cgacattgta aatatggtca tagcggcacg
    33301 gggcgaaatg gtggagtcag gaaggttgcc acaagctgtg aaaaagtttc ttgaaaatat
    33361 agaattatat ggaaacccct atggtgaatc cagggccaaa cgaggtgagt gggctaacag
    33421 gatgggtgtt gaaaaatacc atggccagga gtatcttctt tatgttggat gcacagggtc
    33481 atatgacaca cgggcacaaa aagcagcgag ggctttggca gagataatga tgagagcagg
    33541 ggtttcgttt ggcgtgcttg gtcctgaaga aacttgtgat ggcaacgagg tatacaaact
    33601 gggagaatcg gggctgttcg aagttttggc tgaaaacaac atacagaagt acaaaaacac
    33661 gggtgctcaa aaaatcgtga ccttctctcc tcacgcatat aatgcgatga aaaactacta
    33721 ccatgaatac ggtggatctt ttgaagtttt tcattatacg caatttctcc atggcctcat
    33781 tgaaaatggg aaaataaaca atttaagatg tcctaatatg aaaataacat tccatgatcc
    33841 ttgctttttg gggcgttgga atgaagaata tgaggcccct cgggcaatac taagggctgt
    33901 tgagggtgta gagtttaccg agatgggtaa aaataaggaa agtgctctat gctgtggtgg
    33961 cggcggggga aacttccaga tagacctttt agggggcagt gagaacagtc ctgcccgcag
    34021 aagggcaaga gaggcctacg aaacaggtgc aaacgttctt gctgttgcct gtcccaagtg
    34081 ccttgtaatg ctgaatgatg ctgtcgaatc tgaggaactt gcaaacaggc tggaagtatt
    34141 ggatatttca gaaatagtca taaaggggct caacatatga atttgtgtga aacagacgca
    34201 ggaatgtcgt attgaacatg attaatgtac ttatcatata ccacacccag tctggaaata
    34261 cggaaaagct tgcaagggct gttgcccagg gagttaatga tactgaaaat gcatatgcaa
    34321 ccttcaaaaa tgcctccaat gcaacggctc aggatgtaag gggctgtaat gcgttcgtga
    34381 tttgttcccc tgagtatttt ggctatatgg ccggtgccat aaaggatttc tttgatagga
    34441 cttacgaaga actaaaagac gatcaaagga tatggaaaaa accatactgc attgttgtta
    34501 gcgcaggaaa cgatggaagc tttgcccatt cccacataga gcgcatttgt aagggctatc
    34561 gttttaaaaa aatacagcac cccatcattt gtaagggtcc tgttacagaa gaaatggtca
    34621 ggaaatgttt tgatctcggc atgaccattg cagaaggcat caacgcaaaa atattttagc
    34681 tacctctgtt tcatagattc aggtatcctg acagcaatta cctctccacg ggcgcatata
    34741 gagccttttg cgaggacctc caccaaaaca gatactttgc gttttccaat ctcttttacc
    34801 ttccccctag ccataagctg aacacccagg ggcgtagggt tgatgtaatc cactttcagg
    34861 gaagctgtga caaagcgcgg cctcggctcc ttatccattg ttctgccctc cgagcgtgac
    34921 atcgctgcag ctgccgtacc tactccatga cagtcgatga atgatgctat cagcccaccg
    34981 tacacaaaac cgggggttgc tatgtgatag ggttcaggcg tcaggtaggc aaccgattcc
    35041 tcgccatccc agtaactctt gatatgaagg cccatctcat taaggtttcc gcacccataa
    35101 caatggctca gatctggagg atagtagtct tgaaatgact tttcttctac catatcatcc
    35161 tccttgtttt gacaatttgt tctttaacct gttataaatt caaaacaaca ctgaaaggag
    35221 tatatcatgg aatatgaaaa tattcttgtg aaaacaggtg atgatcatgt gggagagata
    35281 gtgctaaacc ggccaaaaca gtggaatgcc tttaatactg ccatggccgg cgagcttgaa
    35341 cgggcactat gggagttcga tagaagcaaa aatgtcagag taattctttt gaaagctgca
    35401 ggaaaagcat tctgcgtcgg aattgatgtg agcgaattct ctggtaaaac agttatggag
    35461 taccgtaatt ggattgggca tatgggaaaa gcaatgatta cgatgagtcg tatctgcaaa
    35521 cctgtcattg catcagccca tggcatggta acagccatcg gagccggact tgtggcagcc
    35581 tcagacctgg ctattgtctc agaggatgca aaatttggtc taacagcaat caatgttggc
    35641 cttaactgta ttgggcctgc cgttcctgtt gctcgatccg tgggaagaaa aagaacgctt
    35701 gagcttttat tttttggcga ccttataggt gcggaaaagg ctttggagtt tggctttgtg
    35761 aacagggttg ttccagttga ccagttggaa caaaaatcac gtgaatgggc tgtaaaactt
    35821 gctcaaaaaa gtcctctcgc cctccagatg gcaaaaaaag cattctacct ggctgcggac
    35881 atggattact acaaagcctt tgaatgcatg acggataacc ttgcgcgcct gtgtaccacg
    35941 gaagactgcc aggaaggtat caatgctttc atggaaaaaa gacagcctgt ttggaaggag
    36001 aggtaaattt gaaacattgg agttacaatc cttttgcgac attaccggct tgacaataac
    36061 ctcattgctc tataattatc ccaaaacctg catttacctt caataaacac ttaacaaaaa
    36121 cctcttcttg ccgtagaatc aattgcctgc cagacaatct acccagtaag aggaggaaaa
    36181 aaaataatga aggcaaacac tgcagggtca atgaagagtg ctccccccaa tcatgatatc
    36241 tgcaggaaga ttattgccct tgttgccatg atctttgcca gtgcaaagcg gttctcccgc
    36301 catatgtact tgggtgataa agatatcctc acacaggagt ttagcttaaa gagatcacct
    36361 gctacagtag caactcttat ctggatgtcc aataacctaa aaacaatcat ggtggcagaa
    36421 gctggcttgg ttttatgata tcaaacaaat atgttacatt ttgtatgacc cataagaaga
    36481 tacagggata tggaaaagag taagcaaaaa ccgaggtgga tattcacacc tcagcagaaa
    36541 ttcaagatca tcaaggacat taagcgatgc acctccatcc aggaagtcct gacaaagcat
    36601 ggtatttcga acatcatgtt ctaccagtgg aaacgactgc ttgccatggg ggtcaacacc
    36661 agttttgccc aggtacgaaa gacctataag caatgcttta accgaatgat tcttcggcac
    36721 cataaaacag gaagcgatcc acaaagttgg cagctatccc ggtgaacagt cagaccagta
    36781 tgagattggc agatatatcg acatcctata ttaccgagtg gctacatcag ggccttagga
    36841 actgtaccct tgcttttgtc atgagacaaa caacaatgca tttcttcttg agaaattggt
    36901 acagctcaaa tacgagaggc aaagatgagg agaaggcata tctgggaagg cacacatcta
    36961 tgatcatcag atcatcgtta ttactgtgga aatgaggaag atcttgtggg atttatggtg
    37021 caggtgtgag aagtgtaact gcaatctgcg ggatgtgcct ttctcacgtt ggcattggaa
    37081 aaacttggtg ttcaatccag gggacaggcg aggcagtagg agaacaatat ggtttcatta
    37141 ttaaggatgc aacagaaacc ttgagagggg tgttcagatg aagggcggga atcttttcaa
    37201 tttttgcgat cttttatgca aatacgcaac tactgcgaaa gaagaaggcg tagatggttc
    37261 agggagctgt cgcacttttg ttgctcttta ctgcaagaaa aaaaaatcac ttgttcacaa
    37321 aaatatgccg tgcattgaga agcgaatgaa aacaagagaa aagttagcag tgaaaagaaa
    37381 aaagtagttc tttataaccg ttcgcttctg gcttctgact cctatctttt cattgttttt
    37441 tctatcctta tcctcgatgt tccttttgcc tttctcagga ctgttgcatc tccaatgatc
    37501 tttcccacca ggtttacttc gctggcaggc actggatccg agccagtact cattggcgtt
    37561 ggtccgaaaa atatggctaa tgcatttcca ggcggccaaa aaccgatgtc accaacttta
    37621 atgctcgttg tacttgtttc atcaagggac atggtcaagg gaatagtaaa gtagaattca
    37681 tcgccccaga cattcgctct ggcatcgatg ggcagaacgt tagcaacagc ctgaccacag
    37741 agagtgtctg ataactctcc gtaaacagtt acttcactaa tggaaatctt tatcatggtg
    37801 ggcatttttt atactcctct tttctcatat cgcccgcctc ttcaaaagtc aagcttattc
    37861 ttgtactctt ttcagcctaa aagtgcgtgc attgaggtct gggttgattt cccctcgtcc
    37921 ataacttata gtctattgcc agccgttctt aaaatatttt tgttgcaata atttccatag
    37981 tatgggagaa atttaactgg gtaagctatt aaataaaacc tactgcaggc gattaataga
    38041 gaagacaaga taatttgccg cgacagtcac agaaaagatt caaccttttt gaacgaggtg
    38101 gtaatatgga aacaaaacgt attcttgtcg tggacgacga cgaactgatt cggaaatttc
    38161 tcctggactt ctttgttgat cttaaatttg aagtagtcac ggccgagaat ggaacagacg
    38221 ctttgagaaa gtttgcgcct gatagcttcg acatcgtaat ctccgacctc atgatgccgg
    38281 atatgacagg catagagctt ttaaaagagc tcatgataaa agataaaaat attttattct
    38341 ttctcattac aggttaccca acccttgaga ccgctgttga ggccattaaa aatggcgcct
    38401 atgactatat tgttaagcca ttcaatctcg aggacctaag aataaaggtt gaaagggcta
    38461 taatgacccg ccagctaaaa agttctttaa aaaatgtgag cggaatccta tgggcgctca
    38521 ttatatcggt acctatctgg atcatccttg gcataatctt gggattcgtc tggaaacatt
    38581 agtaatttct ttcgatggat atcctaaaac ctaacaaact tgtcccgggt gacaccatag
    38641 cagtagtatc ccctgcgtca ccctcgctca cacctaacca tatggagcgc ggcattgagg
    38701 cttttcagca gatgggctat cgtgttgtta cttcacagca tgtccactat cggcacttgc
    38761 tttttgccgg tgatgaaaag gcacgtgtta gcgatattca tgctgcattt caggacaagt
    38821 ctgtacaggc gatcatctgt gcgcggggcg gctgtggcac agcacagatc cttccccaca
    38881 ttgacttctc attaattgct aaaaatccca agatattcgt tgggtacagt gatataacag
    38941 cattgcagac tgccattttc aacgcaaccg ggcttgtgag cttttatgga cccatggtag
    39001 cttcagattt tgggaaaagt cacaaccgtt acaaaatcaa gaactttttt aaaatcctta
    39061 ctgaaacaca acagacgata gagttgaaaa atccaccttc aaaaaagatg atcacccttt
    39121 accccggggg tgcagaaggt cggcttgccg gaggttgcct ctctgtggtt gccgcaagcc
    39181 ttggcaccag gtatgagatt gagacggagg ggagaattct gttttttgag gatactgatg
    39241 aacagcccca tcgtattgat cggtacctga cccacctcat tcaggcgggt aaacttcaga
    39301 aggctaaggg gatcatattt ggacccttcc caaaatgcga gtatcttgcc agggacaact
    39361 atttcaaatt taaagtaaag ctcctcgacc tgatcaaaga gaggatagtc cctctcggca
    39421 ttccctcaat ctacggcctc cagttcggcc acgttaccaa caagctcacc atccccttcg
    39481 gtggctatgc ttccctcgac gcaacaagct gccgcgtctt tgtggaacca tgtgtggttt
    39541 agctgctgct catcattaaa cgctgtgcct cacgcaaaaa actccgggga atgccctgca
    39601 tcatgttcat gctctttcag ggctctgtct ttacgctgaa tagctgacga agaatactga
    39661 actggcacgg cagcaccctc acctacgcca gggtcgttat gtcgccgaac tccatgcatt
    39721 tcctgaaaga cttgtaccgt gccttgatct catcgtaggt cagagccttg atacggttga
    39781 tgccaaaatc ctcaacactg aaggaggcca taagggagcc cataatgatg gcctgcttga
    39841 ctgtctccag tgatgtatca cccatgtttg caaggtatcc cataaagcca cccgcgaagg
    39901 tatcacccgc gccagtaggg tcaaacacat cctcgagtgg aaaagcgggg cagagaaaaa
    39961 cttcatcctt gctcaccatg agaacaccat actcgccgcg ctttgcaata acacaggagg
    40021 ggccaagctc catgattttg cgagcacccc tgacaagatt atactctttt gatatttccc
    40081 tcagttccgc ctcgtttata acgagcatgt ggacccgttc tataactttc atcagctcat
    40141 ggaatttatt cccgatccag aagttcatcg tatcgagcac gacagcttca ggggattcta
    40201 cctgatcaat aacgtggagc tgtaacgctg gatcgatatt ggcaagaaaa aggtagtttg
    40261 cctttttcat ttcttccggt atgtttggct tgaatgattc aataacgtta agttcggtct
    40321 tgagggtgat cgcctggttg aggtcatacc cgtattttcc ttcccagcgg aatgtcttac
    40381 cgtcatcgat ctttattcca ctcagattga catggcgatc tttcaaaaaa ttaattgcgc
    40441 tgaggtcaaa gtctctgccc actgttgcca caacgcccac atcagtaaaa aaactggctg
    40501 aattggtgaa gtggagagcc gaacctccaa gaacattctc cacctttcca aaaggagttt
    40561 ggatggaatc gtatgcaacc gtgccaacaa cgagaagttt gatcatgttc ccatctccca
    40621 ggaagccagg tatttcttct gttctgcagt caacttgtct atcttgatac caagacactg
    40681 gagctttagg aaagagacgt tctcatcaat ttctttggga acgctgtaga ccattttctg
    40741 aagcttcctc ccatttttcc acatgtattc ggagcatagt gcctggtttg caaagctcat
    40801 gtccataact tccgaaggat ggccttcggc actcgccagg tttaccagtc tgccctcacc
    40861 aagaaggtat attttcctgc catcagccat ggtgtattca tccatctgtc ctcttatttt
    40921 ccgcttcttt cttttcatag tctcaaggga ttttatgtct atctcaacat tgaagtgtcc
    40981 agcattaccc acgatggctc catctttcat gagcttgaaa tgctcaccac ggataacatt
    41041 gatgtcacct gtggcagaga tgaagatatc accgattttg gctgcacgtt tcatatccat
    41101 aacattaaac ccgtccatgc gcgcctcgag agcccgcagt ggatcgactt cggttacgat
    41161 cacatgtgct gacagccccc gtgcccgcat ggcaatacct ttgccacacc acccgtagcc
    41221 ggctacgaca aagtttgccc cagcaaagag tagattggtt gcccgaagaa taccatcaat
    41281 cgtggactga cccgtgccgt atctgttatc aaacatgtgt tttgtatacg catcgttcac
    41341 cgcaactatg ggaaaacaga gcactccttc cttttccata cttttcagcc gaattacacc
    41401 ggtagttgtt tcttctgtac caccaacaac tttgttaata aaatcttttg ttttcttctc
    41461 tcccagcatt tttacccact tcagaatctt cggatgaagc gcttcatatc tcttcaaggc
    41521 tatcatgtgg agagaaccta cgaggtcagc gccgtcatcc atggtaatat tgggcataaa
    41581 agcaagagcc ttcaggatat ggctgtaata ctgatcctcg ttctcgccct taatggaata
    41641 tgttggaatc ccaaaatatt tcacaatagc agctgcaaca tcatcctggg tactcaacgg
    41701 attagacgca cagattgcca cctctgcacc accctctttt agcgttcttc cgaggttcgc
    41761 cgtctcagtg gtcacatgga gacaacaagc gattttaacc cccttgagcg gtttttcctt
    41821 ggcaaaccgc tctcttatct tgcgcaaaac aggcattcgc cgctctgccc attcaatctt
    41881 ttccaatccc tggtctgcaa gttttatgtc cttcacatca taatccattt tattcctccg
    41941 tggcagctta cagctactgg ctgatagctc atcgttaata taagattttt tgactaatac
    42001 actccagcgg ttgctattca accgtcaaca aaaagcgtgg ttcttttatg ctttaactga
    42061 aagctgactg ttaacagctg aagttaaatt cctgccatct ttcgtattgc atccaccatg
    42121 tcgagttttt cccaggtaaa tccgggttcg tttcttccaa aatgaccgcc tacagctgtt
    42181 tgtttataaa tggggttgag gaggtcaagc ttttctatga ttggtcttgg tcgcagatca
    42241 aacagttcat taacgatatg tgcgatcttg tctggtggta tctttgatgt gccgaaggca
    42301 ttgatcattg tagaaacagg ccgggcaaca ccaatagtat aagcaatctg aatttcaagc
    42361 ctccgggcca gtccggcagc cacaaggttc ttggcaacat agcgcgccat ataggaaccg
    42421 cttctatcaa ccttggacgg gtctttgcct gaaaaagcac cgcctccatg gctggccatg
    42481 ccgccatatg tatcaacaat aatttttcgg cctgtcatgc cgcaatcacc tttgggacca
    42541 cctacaacaa accttcctgt ggagttgaca aaaaatcgtg ttttttcgtc catcatctcc
    42601 gcaggtatga tctttttgat gacttcctca ataacacctt cacggatagt ctcattggtc
    42661 acatctggat tatgttgggc agcaataacg actgcatcaa ccctcacggg catactatca
    42721 atatattcta ttgttacctg gctcttacca tcgggtctca ggaatttgag ggtttcgttt
    42781 ttcctcacat cagccagacg tttaaccaat ttgtgggcaa acatgattgg catgggcatt
    42841 aactcgggcg tctcatcaca cgcgtaaccg accatgagcc cctggtctcc cgcaccctgt
    42901 tcatggctgt tcgtttctgt tacacccatt gaaatatcgg gggattgctc atcaatagat
    42961 gtaagaacag cacacgtttc ccaatcaaaa cccattgccg agtcattata tccgatctcc
    43021 tttacggtgt tccggacaat ctcaggaatg tttgcataag cactggtggt tatttcgcca
    43081 ccgacaaaag caaggcctgt agttacatat gtttcgcacg ccacccgtga tcttttgtct
    43141 tgtgcaatca gggcatcgag cactgcatca gatatctgat ctgcaacttt gtccggatgt
    43201 ccctctgcca cagattcgga tgtaaaaaga aatttggtca ttcccattat tgtacctcct
    43261 tcatatgagt tgtaagattt ggctcttgga tttcagcaaa gcccacctgc ctcatacgat
    43321 gtgttatctg ctgggagctg atcgctgtga gctgtcttat tcatcgtgct cttgactgaa
    43381 atcattgggg aagagtcttc ccatctcttt tttcaatata tattcgcctt cttttgcgga
    43441 gttcttaatc attaactcat ttaataattc tttacaataa tcctggctaa tgcctctgat
    43501 tactttcttt actcgcggta tggcatatgc attcatgctc aattcatcta ttcccatacc
    43561 aatgagtata ggagcgtata atggctctcc ggccatttca ccgcacaacc ccacttcgat
    43621 cttgttggcg tgtgcattgt caacagtctg ttttataagc cttaatactg cagggtgtaa
    43681 gggctcgtaa agataagaaa catattcatt tattctgtct attgccaggg aatattgtat
    43741 caaatcattg gttccaatac taaaaaaatc aacctctttt gccagaatat cacttatcat
    43801 gcaggcagag ggcacctcga tcataacacc aaccttaatg ttattattga attcgatttt
    43861 attgtgccgt aactcttcct tacattcatc aactatttcc ttggcctttc tgacctcgtc
    43921 tatacctgaa atcataggga taaggattcg caaaggaccg tatgcgcttg cccttagtat
    43981 acccatgagt tgggccttga aaaggcctat ctcccgaacg cacaaccgga ttgcccgcaa
    44041 ccccatggca gggttgattt ctctcggcac ttcaatctgg gatgcgaact tgtctccgcc
    44101 aatgtcgagg gtcctcactg ttacatattg aagggattta ttttcagcga gtttacgata
    44161 aacatggtaa tggtctgttt ctgttggaag cgttttcctc gaaagataga ggtattctgt
    44221 ccgatacaat ccgatgccta cagcaccata gcgctttata agatccatct cagcaagaag
    44281 ttcaatattt gcgcctacct tgagccggaa accgtctcgc gtttccgccc tgagcctcgc
    44341 aatcttcaga aattctttct tttgagcctt aaggtgcagc tgctttacga tataatcttt
    44401 ctggactttt cttgatggat taataatgac aatcccgtta tccccatcaa tgataatgtt
    44461 gtcattattt ttcaccaggc tggttatgtt gcctgcccct acaactgccg gaatttcaag
    44521 ggcccttgca acaatcgagg tgtgggatgt tctgccacct acgtctgtgg taaatccaga
    44581 tattctatta agattgagct ggatggtgtc tgccggcgaa agatcgtggg caacaataat
    44641 gactttaccc cttatcccct gcatttctat agcagattcc ttccctttga tcatgatccg
    44701 aataagcctg ttgtatatga atttaatatc ctggcccctt tctcgtagat atggatcatc
    44761 taccttctcg aagcctgaaa ggaatctgga aaccacgatg tcaagggccc attcggcatt
    44821 tatcttctct gcacgtatgg tgtctatcac ctcgtcgtga aagagagtgt cctgcaaaat
    44881 gagcatatgg gcatcgatga taaaggcatg ctttctaatc tcgtcatcag taacagcgat
    44941 tttgattgcg tttaactcat caatggcgct attaatagca tacttgaatt taacaacttc
    45001 tttgtcgatg gtttcttctc tgatttttcg tttgacaaaa gccacccttc cccgttcaag
    45061 aaggtagacc cttcccgatg taatgccaga agatacgcca atgcctttta gtgttttgtc
    45121 agtaaaggct ccttcttctc tcattcttcc tgaaaccctg cttcgatcaa ttttccaagc
    45181 tcctggaatg cctggttttc atccgccccc tcaacctgaa ggattatctt gcttcccaag
    45241 gggcaggcaa gggtaagaag gcccatgata cttttcgcat tcactcgcat accatctttt
    45301 tccatccata ccttggactc aaaccccgat gctgttttaa caaaaatcgc tgctggtctc
    45361 gcatggaggc ctaatctgtt ccgaatggtg aaaattccct ccaccatgac ctgttaatcc
    45421 ttttttcttg tcttcaacac atcttgagca agatagatat ttttttgtcc ataatctgtg
    45481 atgaaccggg caagttcatc gagcgtcttt ccctcccgat aggaagccag ctttatcaac
    45541 attgggaggt taacgcctga tacaagttct attttaccct cttcaaggaa ggagaggcta
    45601 atatttgagg gggttccgcc aaacatgtct gtcagaatca acactccatc acccgagtcc
    45661 accattttga gcgcctgaat aatcttaccc ctgatctttt caatatcttc ttccggaaaa
    45721 atgcctacag cttcaaactg ttcctgcttc cccacgatca gctcaacagc agcagccatc
    45781 tcctgagcaa ggttaaaatg tgcaaccacc atgattccaa tcataggacc tccatcatcg
    45841 atcgacatcc ctgtgcattg tcgatacatt atatccgttc tctctaagat gttccgcgag
    45901 ggagtcaaca accataactg accggtgttt gcctcctgtg catccaatac ttattgtcag
    45961 ataactcttc ccttcctttt cataaagggg gatcagataa aggacaaact tactgagcat
    46021 gtcaaaatac tcagtatact ctacttgaga gcgaataaat tctttaacct catgagataa
    46081 cccggttttt tccctcaact catcgacaaa atatggattc ggaagaaacc ttacatcaag
    46141 gaccatgtct gcctccatag gaaggccgta ggcagaaccg aacgacatca ggttaattct
    46201 cattttcaca tccgattcac catatgtatt cagaacaaat cgcctcagct catgaagggt
    46261 aaggtgagaa gtatcgatta tgttatcggc tatttctttg atccaaccta acagccgcct
    46321 ttcttcctta agggcatccc tgacgtttgt tgcccccagt attgggtggc tgcgcctcgt
    46381 ttccttgaat cgttttatga cagactcatc tgagctttca agaaaaataa tttccacacc
    46441 atgctttacc tttgcatcaa tcagcagatg cttccaatct tcaaagaaat ccttctcccg
    46501 tatatcgacc acaagggcgc atttagagat tttattccct gtcagttgaa gtaattcagg
    46561 aaatttctcc aaaagtacca gtggaaaatt atctacacag aaaaaaccag catcttcgag
    46621 tgctcgcaaa aaggttgttt ttcctgaacc ggagatgccg ctcaaaataa ccgtcttcat
    46681 ggtttgaatt tgccctccat tgacttgagg atcttatcct ccagttcagc agcagtaaaa
    46741 atgccctgct gcttaaggac atggttcctg ctcgcaagtt caataatagc cgctgtattc
    46801 cttccggagc ttagaggaat ctttactaaa ggcaaatcaa taccaagaat acggtaccgt
    46861 tcctctttca ggcctacacg ctcatactcc atcttctcat tccaatctac aagctctact
    46921 acaagattca ctttctttgt atcccgaact gcagaaacac caaagagatg ggcgatgtcc
    46981 acaattccga ttcccctgat ttccaaaaga ttcttgatca tcaccggagc ttcgccaata
    47041 agatcaacag cacctttctt ttttatattt acaacatcat caacaacaag gcggtgaccc
    47101 ctggtaatta actcaagggc actctcgctt ttcccgatac ccggccttcc cacgatcagc
    47161 acacctactc ccataatatc catcaacacg ccatgaaccg ttgtggatgg cgccagtttc
    47221 tcttcgagca gtcttgttac cctttcgata aaaacagagg tgacaagttt gctacgcaag
    47281 agtgggaccc ccttatcttc agcttcggca atgaggggtt ctggaaccaa gaggtttctt
    47341 gtcacaacaa aacaaacaac atcatgggtg cagagaacgc taacaatatg tttcttctcc
    47401 tcttccggaa gtgagttgag gtaagtgatc tcagagttac caaaaatctg aactctgtga
    47461 ggatggagat aaaccatatg gccagttatg acgagaccta atttctgaat ccttggatta
    47521 tagatttttc gtggtaggcc tttaaagcct gctatgactt ctaactccag tccatataca
    47581 atgtcgttta tgagttcttg aaccgttata cctttcataa agatatagag caccagatgg
    47641 gaagcagggc gttgtatcgt gcgacagtga ttcgggcatt aaattcgacg gtcctcttca
    47701 acaacactcc tgtaaatgtc atgtgcatta tcaagttcca ttagtcgttt tctgaaagat
    47761 gggtcgcgaa gaagcttaga tattcgtgaa agcattttca gatgtaaact tgctgagttc
    47821 tctggagcga ggagcaaaaa aaagatgtgg gccggtttct gatctatgga atcaaaatca
    47881 actcccttga tcgaacgccc aaaggcacaa atgattttcc ctatactttt aagcttgcca
    47941 tggggaatgg caacaccatc tccgatacct gtgctgccga gcttttccct ctcaaagatc
    48001 acgttcacaa cctcttgctc gttctttatg aggtttgcct ttttcaaggc actgacaagc
    48061 tctatcagga cgtctttctt cgcttctcct tgaagatcag ctattacaca tgattcgtgc
    48121 agtacttctt ctatggtcat gcatttttgc ctgttgtttc aattaggctg aaattgccgt
    48181 cttttctttt gtatactatg cagatatttc ctttttctcg gtctctaaaa atcatgaaac
    48241 tattatctga tgcctcaagc tgcatgattg cttcctcagg atccatgggc ttcgcatcta
    48301 tcgtcctggt aatgattgtc tttgcgcttc cctcctcctg gaaagaactg gcatacttcg
    48361 gcttcacttc ccgatgtttt ctcagtttat ccctgtggcg ggtaagtctc cgttcaagct
    48421 tatcgatgat tttgtcaaca gcggcccgca tatcatccat gacttcgtga gcgttaatca
    48481 cagtaccatt taaggtaaat gtcacatcaa ccctcttccg gtattttttt tcaagcgaca
    48541 gaaccacatg cacatcgagc ggagtttcga catatttgtg aagccttgcc atcttttctt
    48601 caacgtacat ccgaatatca tcatccggtt caatatgtct gaagctaata gtggttccca
    48661 taattcctcc aaggataaaa ataaggttgg aagataccca accccatgta caaagttagc
    48721 agatataaac taccatgtca atccaattca ttgaaaccat tctgttatta tactcaacta
    48781 gccataaatg tacataacaa cattttgcaa caaatctcag tcgagaagcc ctcgctctct
    48841 ggatgagctt atgttgagtg catctctgta ttttgccaca gtcctgcgag caattttaat
    48901 actatgacgc cctttcagta gtgagacgat ctcatcgtca gtaagggggt tctttttatc
    48961 ctcctctttt accatttcac atatcagatc catgacaaca ttggtggaga gtggtctgcc
    49021 ttcctctttg ttgatgccag ttggaaagaa gaatttcaac tcaaaaatgc catggggtgt
    49081 actcatgtat ttgttggttg taattctgct taccgtagat tcatgcacat tgatatcctg
    49141 tgccacatcc ttcaggataa ggggtttcaa aaatcgaagg cctttctcaa aaaactcttc
    49201 ttgaaatgta actatgcttt ttgctaccga atacaaagtc ttttggcgct gctgaatgct
    49261 tttaataaac cactcggcct gagtgatctt ctttttaatg tagttttttg tcgttgaatt
    49321 tgttccttta tttaaataga gatccacata gtacctgttc atcctgaggt ctggtatctc
    49381 atcatcattc agaaaaattt ccaaaccatc ctcggtcttt acaacaaaga catcaggaac
    49441 aacgtatgtg atatattcat ctgcgaagtt tctacctggc ttggggtcat atccctttac
    49501 tttttcaaaa acatctttga tcttctctac tggataacct gtacgcttaa caagctcttt
    49561 gaggtttgtt ttttgaaaaa ggtcaaagaa ggaccgtata atgtcttcaa atatagggtc
    49621 tttttcgcct cgtgcctcat actgtatcag gatgcattct cttagatctc ttgctcccac
    49681 ccccggagga tcaagttttt ggattttttt cagaacctgt tcaagaacct cttctgaata
    49741 gcccgatgta ccggaaattt cagaaagagg atatgccaga tagccattat catcaatatt
    49801 ttcaataatc cattcagcaa taacacgttc attatggttg agctctgcaa gaccggcctg
    49861 caaccgcaaa tgatctctca gattgtgtgt ttttctcacc atattttcat agtcaggccg
    49921 ctcccgttct tcgtggtaag cgtattcttc cgatggtgaa tagcgctcaa gccattctgt
    49981 aacatcatcc ttttcattta ctgtctcttc tcgtgtaacc tcctcttgag agtcttcctt
    50041 gggttctttg tctatgagct ctaaaatggg attctcctgg acctcaagct caatggcctc
    50101 gacaagttcc agtcttgaca actggagaag tttaattgcc tgttgaagtt gttgtgttaa
    50161 cacaggcgac agtttttgag tttgtttaag ctccaacatc gatataaaat tcctctccta
    50221 agtatacctc tctcactatt ttatcatttg ccactgcctc tggggtccct tctaacagta
    50281 cttttccgtt gtttactacg tatgctctat cacatatggg taaagaatct ctgacgttgt
    50341 ggtccgataa aagaatacct ataccctttt tcctcaagcc agaaattatt tttttcaaat
    50401 ctgaaacaga tatggggtca attccagaaa aaggttcatc gagcagcata aaccgtggcg
    50461 ccgtcataag cgctcttgct atttcaagcc ttcttctctc gccgccagat agtgagtcag
    50521 catagttatt tgataaatgc tcgagcttga atgattcgag aagctccaac actctgtggc
    50581 ccatcatctc cctttccaca tgatgaaatt caagaattga cataaggttg tctgcaacag
    50641 tcattttctt gaatacagaa ggttcctgtg gaaggtaggt aatacccttt cttgctctaa
    50701 ggaacatggg cagggttgtg atgtcttcat cgtccagaag gatttttccc ccatttggtt
    50761 tgataaaccc tatgatcata tagaacgtgg ttgttttacc ggctccgttt ggcccgaaaa
    50821 gcccgacgat ctcaccaaca ttaaggccca tagtgatatt atcgacaacc tttcttgcac
    50881 tataagattt agaaagggtc tgtgcaacga gtttagaggt gccgttcact attattcctg
    50941 tttctttttt ttattgtcct gccaatgctt ttccaccagc cggttttttg ggattggttt
    51001 tgcctgctgt gccgctatgg tttttttctc ccagcgatga ggcagtcggt ccaaccttgc
    51061 cacctttaaa atgatatacc gactgttcgt ttttagcaat acttcctttt gcgtacatgc
    51121 gcaccttgcc ccttgcatct accttctcaa tctcttcgcc atcagtgtat atataaagcc
    51181 gatcacatgt caccctcagg tcttgtccgc tcatcaaggc atttccatcg aggagatatc
    51241 tgttgtccct catggaatac ataaaggagt cagcagaaaa cctatatttt ccattttttg
    51301 cctcaataaa acctgtaaca tttttcataa gtttaatcgt ttcttccttt gcattggaca
    51361 acaaccctat ccccatcaat gttaatcgac ttccttcaat tttcaccggc gcatctgtaa
    51421 cggctaagcc tttcttgaaa tcgaagttga gatattttgt cgtcagcgca taatcgttgt
    51481 tatggattat gcggacatct tcaactgtgc ctgtttcttg ctctgtatcc atgactcctt
    51541 tgctaccctt aaaacgaaca aacaaatcag actttggctt atactcaccg tctatctcct
    51601 ccatctccac aaccggttta tcaacatgct ttttgcctga ttttccccga atctcccatt
    51661 caattacacc ctttttttct ccagagtatt tcacgtcttt aaagacgatg acggatttag
    51721 cctcttctat ggaaggtggg gcaattttct caggcttctt aaaaaaaaga tatcctgcaa
    51781 agataatgct ggctgccaca aatccggatg caatataaag aatcgctgtc ctgctgttca
    51841 ttttctgggg cttgagttac ctcttccgtg ccgttgtttc acctgcttca acaaccgaaa
    51901 cagttaaaac tattcaaacg acctgcttac tgctccgtgc tcacgcaatc tttgccttta
    51961 aaaggtcatg gatgtggatg atccctacag gccgtctttc tcccactttt tcaacaacaa
    52021 aaagagaggt tattgaaaat tcttccattt ttttcagggc atatgtagca agggcatcct
    52081 ttttgatcgc tttggggttt ttcgtcatca catcagacgc attttttgac agtacatcat
    52141 taaatcgctc aatggccctg cgcaaatcgc catcagttac gactcctaca atttctccat
    52201 cgcggttcat cactgcagcg actccgagcc gttttgagct gatctcgaat attacatcct
    52261 tcatgggggc gtcttgaaag actctgggca gttgatcgcc ggtgtgcatg agttcttcta
    52321 ctgttaaaac taattttctc cctagggtac caccaggatg aagggaggca aagttttcta
    52381 tactaaaatt tctcttatcc atgagagcaa tagcaagggc atcaccgaga gcaagggttg
    52441 cagtggtgct cgacgtgggg acaacattat gcgggcacgc ttcatcaacc ttcacactga
    52501 taacaatatc accatatttt gcaatagtgg actctggatc acctgtaata accaccatga
    52561 taatattcat tctccgtatc cagggaagca ccttgataag ttcttccgtt tcgccactgt
    52621 tactcgccac aatcacaaca tcgccacttt gaaggatccc cagatcacca tgaatagaat
    52681 ctgtaggatg cacaaaaata gcagggactc caacgcttga aagtgtagag gctatttttt
    52741 tgcagataag tcctgattta ccaataccca tcataataac cctgcccctg cattgggcaa
    52801 tagcgtctac agctctttca aaatccacgt tgagacacga catgacgctg aggatggctt
    52861 ctgcttcctt cctcaggact tcttttccaa tatcaatgaa tcggctcata atgccgcctc
    52921 aattcttttt agcgtctcaa gcatgagagg cagatcgtca agacagactg aattagctcc
    52981 atcgcataat gctttcgatg ggtcaaagtg gacttccatg aagatggcgt caacgcccac
    53041 ggctatcgcc gctttcgcaa gaggaaagac aaattcccgt tctccgcccg aaacagtaac
    53101 catacctcca ggtttctgca cactatgagt agcatcgaat attatagggt agctgaatct
    53161 tttcataatt ggtatggctc tgaaatcgtt caccagatta ttatatccga aatacgtacc
    53221 ccgttcggta accatgattt taaaatttcc tgtcgactcc actttattga tggcgtgctg
    53281 tatatcgtga ggagcaagga actggccctt cttgatattt actggcttac ctgttctacc
    53341 acacgccaca aggaggtctg tttgccgtga caacagggca ggcacctgta aaacatcaac
    53401 cacggttgcc gccgttttcg cttcctccac agagtgaaca tcagtaagga ttggaacttg
    53461 aaagttattc ttcaccgctt caagcacttt aaggccctca tctatgcccg ggcctctgaa
    53521 gctacttatg gaggttctat ttgctttatc ataagaagat ttaaagataa agggaacggc
    53581 gagtctgctc gttatatctt ttagcctttc agcaattctc agagtaatgt ccgatccctc
    53641 aataacacag ggacctccaa taaacacaaa cggttttgcg ccgactgtta cagcgctgac
    53701 ttttacttct ttctgcatcg ctgtctttct tgcttgcctt tgagtgaggc tttgataaaa
    53761 tccctgaata gtggatgggg gctgaaaggt tttgacctga attcagggtg aaattggcat
    53821 gctaaaaacc aggggtggtt cttaacctcg atgatctcgg caaactgtcc atccggtgat
    53881 atccctgtca cgttcattcc acatgcttca agcttttttc gaaatgccat gttgaactcg
    53941 taccgatggc gatgtcgctc gtagatcaac ttctcattgt aggctttaaa tgccaggctg
    54001 cctgctttta gcttgcatgg atatgcacca agcctcatgg tggctccttt gtctgaaaat
    54061 ctgtctctct tctgcacctt gttcttctga ttaacccatt cgtcgataag gaagattagc
    54121 gggctacttg tagccgtatc aaattctgtg ctgtgggcgc ccttgagccc tgccagattt
    54181 ctaccaatct caataacagc aagctgcata ccgagacaca gaccaagata tggaaccttg
    54241 ttctctctag catactgtat agccttcatt ttgccttcaa tccctctatc accaaaacct
    54301 cccggcacaa gaatgccatg gactccctgg aactgtattg taaggtctcc gcctttctcc
    54361 aggtcttcag agtctacata atgaatattc actcttgcat tattggcaat tccgccgtgg
    54421 acgagtgctt catggagact tttataggag tcctgaagct taacgtactt gcctactata
    54481 gcaatatcaa catacttctc agggttcctg atggtatcga ctaccgcctc ccacttggaa
    54541 aggtcaggtt tctttgccca aatattcagg agctgggtga tcttctcgtc gaggccttcc
    54601 cggtggaaga ggatggggca ttcatagatt acatccacgt ctttcccggt aatgactgca
    54661 tccttatcaa cattacagaa aagggcgatc ttatccttca aatcctggga tagaagtctg
    54721 tctgtcctgc acaacaagat atctggctgt atacctattc ctctaagttc cttcacgcta
    54781 tgctgcgtag gtttcgtctt catctcatct gcacttttga taaacggaac aagggtgaga
    54841 tgaataaata acgaattctc ctttccaagg tcgttccgca gctgacgtat tgcttcaaga
    54901 aaaggaaggc tttcaatgtc gcccactgtt cctccgattt ctacaatggc aacgtcagct
    54961 ccgtcattaa catcaagaat tcgttttttt atctcgttgg tgatatgcgg gatgacctgt
    55021 acggtcccac ccaggtaacc cccctttctt tcccgcataa taactgcatc atagacctgg
    55081 cctgttgtaa aattgctttt ctttgatcct acagagtttg tgaacctctc ataatgacca
    55141 agatcaagat cagtctccgt gccatcatca gtgacataca cctcaccatg ctggaatggg
    55201 ttcatggtgc cgggatcaac gttgatgtat ggatcaagct tttttatggc taccgtgaga
    55261 cctcttgatt cgagcaaggc ccctatagag gctgaagcga gcccctttcc caaggaagaa
    55321 acaaccccac ccgtaacgaa tatgaatttt tgtctcatat tcagtggcct gtgataaaaa
    55381 acttgatcaa tgcttctccg tcaaacaatc gctcgcctgc ccgttcatca tacccagatg
    55441 ggcatccacc tatcctgtta tgtttctgga atacggtaaa gggaacaggg gtggcatggt
    55501 gggttttcat agcaataggg gttgcatggt cagtaacaat aagaaacttt acaaaatcgc
    55561 caacgctctc gaaaattggg cccactactt ccctgtcgat attctcgata gccttaattt
    55621 tttcgtcaat atttccttcg tgtgaagctt catcaggtgc ttcgatatgg atatatacga
    55681 tatcgtggtc acttaaaagt tcaattgctt tatttgcctt acctcggtaa ttagtgtcaa
    55741 gatatccggt tgccccctcg actctgggag tatcaaatcc agcaagcctg ctgataccct
    55801 tgacaaggtc tacggccgtg acagttgcac ctgtgaggcc atgattgtct ttaaacaaag
    55861 gaaattgggc ttttttcccc tgcccccaaa gccatatgct gttggcagga tacctggaag
    55921 ctttttctct ttttctgttt aacgaatggc cttcaaggat tctccgggag tcaatcatca
    55981 aagagatgag cctgtcagcg cctttacctt tggggaggta atcaagtatc tcttttccgg
    56041 ttatatcatg aggaggagtt gtttccattt cccacatacc gtctttccag atcatcacat
    56101 gacgatagct tactcctgga aaaaaaataa actctttgct accaagtcct ttgttaaggt
    56161 cttgaatcag tatgtttgct tcttctgttg tgatatggcc actgctgtaa tcgcccatga
    56221 aggtcttatc aggatgtata gcaaggtaaa ctaggttgca gcggaaggct acgtcgtttt
    56281 tatcaaggtg tatgcccata ccgcttgcct caataggtgc tctgccggta tagtatttag
    56341 cggggttgta gccgaataag gacatgcacg caacgtcact tcctgacgga taaccatccg
    56401 gtatagtgag aacttttccg cagtaaccat cctgggccat catatccata tacggtttat
    56461 gggctgccat gagcggcgtc ttaccatcga gtctctcaat gggccagtct gccatcccgt
    56521 caccgataat aacgatgtat ttcatggtgc agcacctttc agacactgct ggtttgagtt
    56581 ttgagctttg agtttttcgt aaatccaggg aaattctctt acttgcattt cacaactggt
    56641 gaactgtcca ttaactccaa actctgaacc ctgaattccg aactgttttt tcacagtctt
    56701 ccctcttcta tccgtataga aacgctctcg cctttaataa cagccaggcg gtctattgct
    56761 gcctttgcat ttttcaggtt ttgctccgta gcttcatgag tgagtattac aatgggcaca
    56821 tagccgtttt ctttgcggcc tttctgaatt acagaggata tgctgatatt atattcagcc
    56881 aagacaccgg agacctttga caaaacacca ggtctatctt ttgcagtaaa tctcatgtaa
    56941 tacgggacga aagatttttc tccgctcctc atttttttcg cctttgacac tgaagggatt
    57001 tttggtaacg gtgaaccgcc tctcattcta taagccatat cgacaatatc actaaccact
    57061 gcactcccgg ttggttcgct cccagcccct tttccataat agagcgtcgg ccctgtcttg
    57121 tctcccacaa tgtacactgc attatagacg ccatccacat tactcatggg gtgcacaaca
    57181 ggaatcatgg caggctctac cctggcctca atcttctcat tctcttcttt tgcaactgcc
    57241 aacaacttga ttctgtaccc aaattccctt gcaaactcta tatcgatagg atctattttt
    57301 gttatccccc cagttacaac atctctcatc tttacgtggc attgaaaggc caaccgtata
    57361 agaatacaga gtttatgggc tgcgtcgatt ccttccacat caaaggttgg gtcagcttca
    57421 gcaaatccca gtcgctgggc ctcttcaagg gctttttcaa aggacattcc ctcgagggtc
    57481 attttcgtca aaatataatt actcgtcccg ttcagaatcc ccaggatgta gttgatacga
    57541 tttcccacca ggccatcacg tatcgccttt attaatggta ttccaccaca cacagatgct
    57601 tcaaaaccta tttcaacgtt ctgttttgcc gctaatgtga ataactcatc tcccttttca
    57661 gcgagaaggg ctttgtttgc tgtaactacc catttcttgt tttggatagc catgcggata
    57721 tactcattgg caggctctat tccacctatc agctcaacaa caatatcaac atccttacac
    57781 tcaataactt ccctggcttc cgcagtcatt attccctgct tcagggatat atctcggggc
    57841 cgttttgtgt cgatgtcagc acacttaaca accctgatat cttgtcctga tttgtctttt
    57901 ataagggaat gatactgttc gagaatttta tatgtgccga tgccgacagt gcccaaacct
    57961 ataattccaa cgcctatcat ctgctttttt tcagaataca aatgatcttt gattcttttt
    58021 tcctctatct tttgttctat attcctcttc tatataagaa aagtaaattt tcactgtgtt
    58081 cacagaaaac ttgcaggaga gactatgcca attatggcct catgaacaat atatgccagg
    58141 gcacgtttca ctgatgaaca ttaccataca accattgaat aatcaacacg aaaagctctg
    58201 tttgacaagg ggtaagctgt tagcactcaa cactcaacag tgaactcaaa acctctcagt
    58261 acagtctgac ctgaagggcg tcattttctg gcaaacctct ccttttcaga gtttgtaccg
    58321 gaacttgcga agaagttcct tgcgggcctt tttgtcttct tctttttcaa ttgccttggg
    58381 ccgaaaaccg tcaatgacac cgagtattcc gcccccctgt tctgactctg ctacgataag
    58441 ctggagggga ttggctgttg cgcagataac atggcaaacc tcctgacata cctttatggc
    58501 attcaataca ttgatcggga acccctcttt caggaaaaca acaaatgtgt gcccacaacc
    58561 aatggagaaa gatgtctcgc tagccagcct catgagttcc tcgtcatttc cttcgtatcg
    58621 agtaaggcaa gggccacttg cttctgagaa tgcaatgcca aatttgaggc ttggagaaga
    58681 acctacaaga atctcataaa gatcttccac tgtcttgaca aagtgagact ggccgataat
    58741 gatgttgagc ccttccggca cattgatttc tactgttttg atgtccatgc ttacctcctt
    58801 gtattagcga attgctgata ggtttcagcc taagccaaat ccataatagc cttttacaat
    58861 cagcttctca gttcttgctt taaaaacggc tcttccagtt caaaatccgg cacataggtc
    58921 ttgacactct caagatcttg aataaaaaca ttattaaaac cttcttcttc aagaaaattg
    58981 agcaaaaaat cgtactcctc ctgccgaata cttctcgcaa gcattgggta ttcagtcgct
    59041 ttatgcattg ggttgtattg cgccatgagg cttataaaag tatctacacc aagttcttct
    59101 tttatccaga gaagaattcg atttgaacct gcaaggttgg aaggaaggac aaggtgacgt
    59161 acgagagtcc cttttcgagc tataccctcc ttaacaataa gatttcctac ctgctgtttc
    59221 atttggagaa tagccttctt tgctgactca gggtagttgc tggcaaagga aagttgctca
    59281 gctatgttac tgttccagta tttaaaatca ggtagataga tatcgatcag tccatcgagc
    59341 atcttcaggg cgtccgctgt ttcgtatcca tggctgttat agacaaaggg aatttgcatg
    59401 cctttgcttt tggcacattc gatggcttcg gcgataaagg gcacgtacgg tgttgggcta
    59461 acgaggttaa tattgtgaac acccatatct ttaaggctaa agaagatttc gacgagccct
    59521 tccagggtga cgctcttccc tttgcattcg tgactaatct ggaaattctg gcaatagacg
    59581 caccgaagat tacaggaaac aaaaaatacg tttccagaac cacgtgtgcc agagattggg
    59641 ggctcctcac caaagtgggg gccgaaatga gcaacgagta tttccttccc tacgccgcaa
    59701 tatccaacat taccccgaat cctgtttact ccgcaggccc tcgggcacaa tctacagttt
    59761 tccagggttt ttcctgcatc tttcatgaaa ctattatatt cggtttaaga aaagaatagt
    59821 aggaaaagat aagaaaaact ttttctcact ttcatggaag aggggatacc tccagagaaa
    59881 aaggggatgc tgtccaagag catccccttt ggtaacgata gcgtggcctg tattactttt
    59941 tcaggaagtc aaggcagaat ttcagggctt cgttgcctgg caaacctttt gcctggactt
    60001 ctgcggcata tttttcatag attggtttta cagctgcagc ccatttggca tcctcttctt
    60061 ttgaaagagc tataacctta tggccttttg ttgctgacca atcataccct aatttatcaa
    60121 tctcatccca gagctttccc tgtttatctg catattccgc acttacctca ttaaatatct
    60181 tctggatatc cggcggcagg ctgttccatt tgttcttatt catgacaacg aacatggtcg
    60241 ctgtatatgc agagccgaag ttctgggtcg taaatttaac gatctctccc catttccaac
    60301 cctctaatgc ttcctgagga gacatggcgc catctacaac gcctgtgcgg agtgcatcat
    60361 aggtttcggg catagtcgta ccaacaggcg ctcctccaag agcctgaaca attggaacaa
    60421 cagttccctg ggcccttatc ttcatgcctt tgagatcttc cagcttgttg acggctttct
    60481 tggtgtggag gattccaggt ccatgagcgt ggagataaag cacatgaaca tcatcaagct
    60541 cttttggttt aaacttcttg tagaattcat tgatcagctt tgttgcctga acaccgctct
    60601 tatagccaag tgggttgtcc ataatactga tcataggaaa acgtccccta gtatagccaa
    60661 aaacactaaa cccgatatcg gctattcctt tagtaacgtt ttcgtaggtc tgggcagccg
    60721 gcgttagggt gcccccagga aaatacgtga ttttaacctt tccatctgtt cttttctcga
    60781 tttccttgca ccatgcctca gccagctggc tgttcttgtg aggcgccggg aagaagttgg
    60841 agtaattgag tgtgatcgtc ttctgttgtg ccgcagacaa acctgggaac gcaacaaaac
    60901 agattgaaac aacaaacaat aaaagaaaac acttcaccat caaacgtcct ttcattagat
    60961 acccccttct aggttttttt tctttataag caatctcttc ctctttgtca atcattattt
    61021 tccacaacac atccatcatc tgcatctcct taatatcctg ttaagccctg attaatgcct
    61081 aaaatattta tatatgcttg tttggctcca acacctcctt ttcttcttcg gtaacacaca
    61141 ttttgcacag cccatgccag ggaatatgcg attttggaat acattatatt tacaagacaa
    61201 ttatgtttta tagcctttca acaactgctg tattggtcgg aatattccga cattaaacgt
    61261 tttacatcgg agaaaattcc gactcagcgt tcgttttaac attaggggca tggcattgga
    61321 agctccctta acaaattcga tacagaccag ccttaatcaa catactcctt gctttatact
    61381 gattcaggtt cagcaccacc atttacttac attgctccca tcagcctccc aggaagccat
    61441 gttgccagtg cagggaatat gaaaagcaaa atagcaacaa aaaggagaga gagcagaaaa
    61501 gggtaaaccc ctttgtatat cacgctaaag ggtgttttgg taatgttctt caccacaaag
    61561 acattgattg ctacaggtgg caccaccacc ccagccatga cggtgataca aatcatgatg
    61621 gcaaaccaca tgggatcata acccagtttt tggatggcag gataaaagat gggcgttgcc
    61681 agaatcataa aggcaaggtc atcgataaat gatccgccga gaagataaat caatgcgata
    61741 aataccagga caagccatga tggtagaggg aggcttacaa tccaatcagc agcgatcatg
    61801 ggaatttttg taactacaat gaagtggccc aggactgttg atccggcaat tagaaaaaga
    61861 accatacatg ctgtcctcac cgactccaga gcagatttcc agtatccttt aaaattgaga
    61921 tcgcgcttgg caaaaaccag gattaggacg aggaatgttc ctacactgcc tgcctctgta
    61981 ggggtgaaaa atccctttaa tatgcccgct ataacaagaa caaatatgag gatgacccat
    62041 gccacctcag gcacagactt aatgcgcact gcccacgaag attttgggcc tttaggacct
    62101 agggcagggt tcatcttgca ccatccgtag attatcacca caaaaaagaa cgcagagatc
    62161 aatccaggga ctatccctgc aagaaaaagc cgaccaatgg actggtctgt aatgatgcca
    62221 aatatgataa gtgttacact cggaggaagg agtaccccga gggttcccac tacagccaca
    62281 ataccggttg aaagcctctt gtcataactg tatcgatcca tctcaggcac agccacgctt
    62341 gcaaatgttg cggctgtagc aggtgaggaa ccgcagatag acttgaacgc tgtcgctcct
    62401 gctaccgtgg caatggcaag accaccggga acatgtccga taaatttata tgcagcatca
    62461 aagagtctct tcgcaatacc ggagttaaag gctatctggc ccatcaagac aaacaaagga
    62521 ataactgtaa agctatatga gttaaaaaca tcaaaaacat cctttgccag caggttcagc
    62581 gctgcatgga atgatatgag gtaaccaaaa cccagaaatc cgataaggat catggcaaaa
    62641 gcaagctcaa tcccagtgag aaaaagaaga agtacaatac ataaacctat gataccaacc
    62701 actacctcat tcatatttac ctcctgctat cttcacaata tcacaaatga gcaccaggca
    62761 ctgtatgaag cagcaaaccc ccaggccata agctaccgga taaaagggca taagcagggt
    62821 tgtcgagact tctccggatt ttaccatgtc gataccaagc acaaagaggt tccagccaac
    62881 aaggatgaaa agacccatgc ttatcagcct agtaactatg ttgaatccat ctctaaggct
    62941 atctggaaac atgttgacca ggaaatctac aaagatatgc cccctgttcc aggatgtaat
    63001 agggataccg aaccctatga taacagcacc cccgaggcct accagctcaa aagtcccgac
    63061 aatgggccgc gcaaaaagcc tcagaaagac atcgctcaca gtgagcagca taatgaatgt
    63121 gagcgcagca cctgcaattg catgcatcca attgcttagc cgtaaaacaa cgttaagaaa
    63181 tcctcccatg actcctcctt acttccggca gttgcttgcc tctttgattt attcaagaac
    63241 actgtaattt ttttatgaaa tatttcatgt ttgaccgcat tttgtcaagt taaataattc
    63301 tgcgcgaact catgaattgc cggaattaat gactctgtct tgattagaag tttcgtatac
    63361 agtaccccaa aaagattcca tactatgagc ttttcttttt tgattcagac caatgcttct
    63421 ttactcccct gtgaaaagat ggtaaataac tttccatgag ttttgaatat gccatgctcc
    63481 cgaatagaaa agaaggcata cctcataacg tgggcgcgtc atctgccctg tactatgtaa
    63541 ctataccccc gcatatcaac cttgaagccc gccatgaagt attcgggaaa caaggcatta
    63601 caagtccacg taacggatca tcggattcca gtctcaatac caaggagaat ttatcccttg
    63661 ttcaaaaccc aggcaacaga tgagactatc gccaagatga ctttggatga tcttaatggt
    63721 gtcatggaga tagaaaagct ttcctttatt tctccatgga cagaacgatt ctttgaagaa
    63781 gccctggcct cacccgtctc gttgagtctg gtattgagga atagtacaca gcccatcggt
    63841 tacatcatcc tttattcggt tgcggatgag gcacacattc taaacattgc tattcatccg
    63901 ggctttcgcg gtaaagggtg tgcttcaagt ctcattgagc atgtaattgg ttgttgtaaa
    63961 aaaagaggtg ttcatcagtt ttttcttgaa gttcgtgaag ggaatgtagc cgccattaat
    64021 ctctacagga ggtttggttt tgagaaaatc ggcaaaagaa agaaatatta tgcggaaact
    64081 aatgaggatg cgttgatcat gtgcctatcg gtacgttagg tcagagttaa aaatcctggc
    64141 actcagagtc ggaaaataag gattgtggtc cccggttaac agtgaagatt gaactgaaga
    64201 ctgtaagcgt tatttaat
//
LOCUS       DAOOMC010000002        54737 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdus sp. isolate AMR_MDS_2685
            NODE_399_length_54737_cov_6.434732, whole genome shotgun sequence.
ACCESSION   DAOOMC010000002 DAOOMC010000000
VERSION     DAOOMC010000002.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864760
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdus sp. (wastewater metagenome)
  ORGANISM  Syntrophorhabdus sp.
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae; Syntrophorhabdus.
REFERENCE   1  (bases 1 to 54737)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 54737)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,729
            Genes (coding)                    :: 2,708
            CDSs (with protein)               :: 2,708
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 7 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..54737
                     /organism="Syntrophorhabdus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_399_length_54737_cov_6.434732"
                     /isolate="AMR_MDS_2685"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2699756"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            93..1331
                     /locus_tag="PK125_00340"
     CDS             93..1331
                     /locus_tag="PK125_00340"
                     /inference="COORDINATES: protein motif:HMM:NF027964.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase family protein"
                     /protein_id="HPB36588.1"
                     /translation="MTNRKNNGRIMIIGAGGVATVAAHKCAQIPEIFKEILVASRTLS
                     KCETIKSNIKKRYNKDIEIAQVDADNLHELVTLINDFQPYLVLNLALPYQDLHIMDGC
                     IQTGVHYIDTANYEPLDEARFEYRWQWAYQERFKQRGLMAILGSGFDPGVTNVFAAYA
                     QKHLFDEIHYLDILDCNAGTHGHVFATNFNPEINIREVTQIVRHWDNGKWVETPAIID
                     EGSVHFSFNYPVVGSKESYLLYHEELESLVMNIKGLKRARFWMTFSENYLTHLRVLQN
                     VGMTRIDEVEYEGCKIIPVKFLRVLLPEPASLGTKYTGKTVIGNIMTGKKDGKKITRY
                     IYNICDHEEAFQETGTQAIAYTTGVPAMIGAMMVLTGVWQGAGVYNMEQLDPDKFMEA
                     LNKYGLPWQVIEHEPLPDRL"
     gene            1356..2699
                     /gene="nspC"
                     /locus_tag="PK125_00345"
     CDS             1356..2699
                     /gene="nspC"
                     /locus_tag="PK125_00345"
                     /EC_number="4.1.1.96"
                     /inference="COORDINATES: protein motif:HMM:TIGR01047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0008216 - spermidine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxynorspermidine decarboxylase"
                     /protein_id="HPB36589.1"
                     /translation="MIRPDKKIKRIIQSILGNDLLAVRDYYALSEDARRKMVTELGPS
                     VLRRLTRLIKKCKKQVFASENKRLLKIAQSDIATPVYFIDEILIEENMKVLRYVKDRT
                     GCKILHALKAYASFATFPLMREYLDGVCASGLHEARLGFEEFKKEVHTFAAAYRDKEM
                     DQIIRYSDVIIFNSFYQLQKYGLRAKKKGIEIGLRVNPGHSEVLTEMYNPCAPCSRLG
                     IINDTFEKYFSGYRDIVEGLHFHAMCEQDSDILERILNSFEKLYGQHIKNLKWVNFGG
                     GHHITRDDYDLERLIKIINGFKQKYGVQVYLEPGEASVLNAGFLASSILDIVTNQMDI
                     AIVDASAETHMPDVLLMPYRPHIIKSGQSKEKKYTYRIAGLSCLAGDIMGDYSFNKPL
                     KRGDKLIFTDMALYSIVKNTTFNGINLPDIAVIKDDKRIEVIKIFDYNDYKNRQS"
     gene            complement(2720..3340)
                     /locus_tag="PK125_00350"
     CDS             complement(2720..3340)
                     /locus_tag="PK125_00350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB36590.1"
                     /translation="MNYFHTTRRFFTVMIFLALIFTGCAQMKTGTSSSNIPVDRAAHK
                     PVLEPSKQTSAQESIPIKRPHVTGDYQKTIDFYKSEQAKYPYDQTLTKDYARNVDEMK
                     DAADQEYEKEKYTSACKTYSILAKNYSAFKTFVHMLSFNRSDINKKLNDCKSILSKKG
                     FQEYREGNLNEAIALWQGYLTIDPNNADIRKAVNTATLQQKNLQKK"
     gene            complement(3340..4599)
                     /locus_tag="PK125_00355"
     CDS             complement(3340..4599)
                     /locus_tag="PK125_00355"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HPB36591.1"
                     /translation="MNNYILNEIVKRGESVSKGIAASAGYSLLSKDLLSLDNLVFKAK
                     VTNNDMEYVAIVAPDMKVVAHSEAVMGGVVMPESQGRIITKRIDGTTVKELMRPSASI
                     FEISCPVVFMKKNMGSVILAINKSVLLEAQAKVRTRILVVFGIIVVLGIIASALLASF
                     LIKPIRELSAGVDELKKGTAKTPLKIYSHDELGKLTSNFNEMSTTIADQQSKLNKYAR
                     DLEEAYVSIVKVVAAAIDARDSYTHGHSARVSQFSVMIGRAIGLSKEELDDLEVACLF
                     HDVGKIKTPDSILLKPGKLTQFEYDEMIRHVEYGASILSKAPSLRKYIPAVKHHHERQ
                     DGKGYPDGLNSDTIPLFASIIAIADTFDAMTSTRPYRSAFTEDVALRELVRVAGTQLR
                     ADLVAVFVGLIDKGYAHEKQKVAGRVI"
     gene            complement(5558..6133)
                     /locus_tag="PK125_00360"
     CDS             complement(5558..6133)
                     /locus_tag="PK125_00360"
                     /inference="COORDINATES: protein motif:HMM:NF014912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin family protein"
                     /protein_id="HPB36592.1"
                     /translation="MDLKGMQTEKNLLVAYHGESNNRNMYTFFAEKAKEEGYEQVAAI
                     FIETANHEYEHAKQILNIIQMRDIELPKVIFPVKGVGNTISNLQTAVSGEHYEKSIMY
                     PDFVKIADEEGFTDVAKILRFITSVEAEHERRFRILLQNMKAGKIFRKDSVVKWKCRA
                     CGYVKKNRNAPKVCPLCRCSRAYFELFTENY"
     gene            6382..6615
                     /locus_tag="PK125_00365"
     CDS             6382..6615
                     /locus_tag="PK125_00365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB36593.1"
                     /translation="MDLKECANCGYKSETSFKDDICPECGKTFWKCENCGYTLTASSP
                     PEACPSCKQKCLFKNITCYTPECGGPGNIDSRL"
     gene            7155..7346
                     /locus_tag="PK125_00370"
                     /pseudo
     CDS             7155..7346
                     /locus_tag="PK125_00370"
                     /inference="COORDINATES: protein motif:HMM:NF013068.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            7343..7558
                     /locus_tag="PK125_00375"
     CDS             7343..7558
                     /locus_tag="PK125_00375"
                     /inference="COORDINATES: protein motif:HMM:NF013068.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HPB36594.1"
                     /translation="MKKKAILTAYGIARQGKGELIDFTLVRHKREYAWVEFFLNLLMR
                     GLAGTNLRLVITDGNQGLVNAVDLTYL"
     gene            8115..8612
                     /locus_tag="PK125_00380"
     CDS             8115..8612
                     /locus_tag="PK125_00380"
                     /inference="COORDINATES: protein motif:HMM:NF014912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HPB36595.1"
                     /translation="MDDRIKAIFKSIYEGEAKAALRLKLFAKQADREDLPQIAQLFRV
                     ISFSEEIHGERALKMLKEIKSTEENLKESFDSETTVAEVAYDQFLKVAADVGDNGASM
                     IFSQSRDVEDIHAKLYKKALDHLMTETQTTYYVCQVCGYVADGMLPDECPVCSAPQNQ
                     FKEFK"
     gene            8824..9129
                     /locus_tag="PK125_00385"
     CDS             8824..9129
                     /locus_tag="PK125_00385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB36596.1"
                     /translation="MNDVIGIAWFKDEPTYRRALDIFTDARNMPATYEDWKTLVKREC
                     EEIKRIGNIALRVDINPEAFTEWCNTHGFPTNSQGRIAFVNHMELEYQKTGQGIIID"
     gene            complement(9336..10217)
                     /gene="speB"
                     /locus_tag="PK125_00390"
     CDS             complement(9336..10217)
                     /gene="speB"
                     /locus_tag="PK125_00390"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR01230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008783 - agmatinase activity [Evidence
                     IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HPB36597.1"
                     /translation="MPEHFVGFCGQLSTQANYHAASIVVLPIPFDKTSTWMKGADKGP
                     QALIDASQYLEFYDIETDGVVLDNGIYTARPIRAASSAVLIKKTDLAVTRYLIDNKFV
                     VALGGEHSVSVGVIKSYARHYKNLSILHLDAHADSRDTYEGSRYNHACVIARAREYTK
                     EIVSVGIRSMDQLERDGMDRKRMFFAHEIFNSETWIQKTVRQLKDNVYITIDLDVFDP
                     GIMPSTGTPEPGGLGWYQVTKLLRAVSMAKRIVGFDVVELCPSHDKAPDFLAAKLVYT
                     LLSYIFADRAVPKLKNK"
     gene            complement(10219..10764)
                     /locus_tag="PK125_00395"
     CDS             complement(10219..10764)
                     /locus_tag="PK125_00395"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: protein motif:HMM:NF013979.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008792 - arginine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006596 - polyamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine decarboxylase, pyruvoyl-dependent"
                     /protein_id="HPB36598.1"
                     /translation="MTPKKIFFTKGVGVHKDKLSSFEVALRSAGIEKCNLVYVSSIFP
                     PKCKIISRTAGLKLLKPGDITYCVMARNETNEPNRLVSAAIGLAVPKNYDSYGYLSEH
                     HAFGEKAVVSGEYAEDLAATMLATTLDIPFDPNQAWDERKQFYRASGHIFTTRHFCQS
                     AEGNKDGLWTSVIASAVFILE"
     gene            complement(10835..11386)
                     /locus_tag="PK125_00400"
     CDS             complement(10835..11386)
                     /locus_tag="PK125_00400"
                     /inference="COORDINATES: protein motif:HMM:NF019498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HPB36599.1"
                     /translation="MKKEDASVGERIKMLRQAKALTQEELATRAGLTKGFISQVERNL
                     TSLSVESLIGILDALDEKPSRFFDGAFDEKIVFKLEDRVEMEIDDVNKFQILIPAAQN
                     RLMDPALMELSVGEKTPIEEPHEGEEFGLVLGGSIDLILGGKVFKLKKGECFYFKANK
                     KHHIENRRKNRALVLWVSSPPNF"
     gene            11505..13088
                     /locus_tag="PK125_00405"
     CDS             11505..13088
                     /locus_tag="PK125_00405"
                     /inference="COORDINATES: protein motif:HMM:NF014795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HPB36600.1"
                     /translation="MTGKQAIIEFFVKNNIHDLFYLPGIHTLSLSQAFSGENINVYVT
                     RHESNMVFMADGFSRVSGKIGVLIVTPGPGLANVVTGCMEAYGDDVPLLVIHIDTGRE
                     GIDKGILHGVAEPETMVRHVTKEIFYVQSTDDLIIKLDSARKVAMTDRKGPVLISIPY
                     TLLEKEVETIRSSEENPVQMQHIDDFDGLEKVLAGKKKPVIIGGKALMFEKARPLLDN
                     ICLHFSIPFLTTTSGKGILDEENVWCFGNIVQKGIAKKIVSSADIIIAIGTRLRDVDA
                     KRRGVKISDLIHVDVDRAWFDKNYSTVYRASGKILPFLHALNEIFTSSSFNWRLKELK
                     EAQHLERLGLQNKFLGFQLINVIKNSIPDDTTTVWDLNLMAYWAEYYYPVRHQHAFIM
                     PRGISSIFYALPAAIGAKTAKPEKPCLAICGDGGVLPTISELSTIRKYNIPVAILVFN
                     NSGFGILEDYMRATYSIEGMMALENPDFMKIAAAFGIKSKRVKTLQQLESTLRQLVQW
                     NEPFIIEFSSPVFAPPWRT"
     gene            13314..14759
                     /locus_tag="PK125_00410"
     CDS             13314..14759
                     /locus_tag="PK125_00410"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPB36601.1"
                     /translation="MKLSIFSRLALGYFVIFVILGIVNGYTLWQLHQISRGTKQIINI
                     DERILEIKKKLVDNILSQMEYEKKFAITKDSIFYEQFLSSQSDFQNYLKDALYIANTK
                     DKTTYLNQINNSYSLYNALVIDDLESIKNKKHFSIVKNEIEKERLVDNILEDLKALET
                     SIMKDVHRRMNVLSEAGSSSRKLTLIMWVTALVLVLFTSFFTTRSITKPLTSLMEKTK
                     EISKGIFKSDLKITSPPEMMELTSAFNAMCDKLNKVEKMKSDFFSTMSHELRTPLTSI
                     KEGIGLLQEGVGGTVTEKQQKLLSILTEESNRLIGLVNSLLDIAKMEAGMMTYNFGHE
                     NIGLLIQQSIQEVAPLVESKKIKIWLETTDELTHPRIDRERMLQVLRNLISNAIKFSS
                     EHGQITISAYLEERGLLISVQDTGPGIPKENLYAIFGKFQQPPVKTSEWTKGTGLGLA
                     IVKNIVVAHGGEVWVESDIGQGSTFFVLLPF"
     gene            14819..15289
                     /locus_tag="PK125_00415"
     CDS             14819..15289
                     /locus_tag="PK125_00415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPB36602.1"
                     /translation="MSLSIEPIHPAAVQVPPKKEQPRDESKEHLLAGQRLLSMGQYDA
                     SVKEYLRVLSIAPNKPPGDEASFAIGLIYAYPKYSKKDHSKAVSSLTRIIKDYPHSNW
                     SGHAKILLDLIQDNERLRRISAEAIHENEKLKNMIEQSKKVDLEIEEKKREKAR"
     gene            15298..16686
                     /locus_tag="PK125_00420"
     CDS             15298..16686
                     /locus_tag="PK125_00420"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HPB36603.1"
                     /translation="MTLGRILVVDDDKNLVDLIQMRLETAGYDVATALHEEYAIEKVK
                     EQLFDLAIVDLQLVNTDGISLMEDLHRIIPEMPVIILTAYGSIESAVEAIKRGAYSYV
                     TKPFEHQELLLQIQKAVENRRLTSEIERLKDLLSERYSFSNVVTRSERMQKVLETISR
                     IAPTESTVLLLGESGTGKDLIAKVIHLASQRKDKPFVAINCAALPETLLESNLFGHEK
                     GAFSGAIRSTKGLFLQADEGTIFLDEIGDMPLSIQAKVLRVLQDKQFYPVGSEKLVEV
                     NVRIIVATNKNLEEQVEQGLFREDLYYRIHVIPIHLPPLRDRKEDIPPLVDHFLNKFR
                     DKVKKDIKGFTPQAMQKLMLHDWPGNVRELENVIEYAAAMTDTELIDENLIFQTKVIP
                     SITYIKPLKEAKDVFERGYLIYLLETCKGKVSEAAKLAGKYRADFYLLLKKHDLNPDD
                     FKTKGKRRSEPT"
     gene            16928..18043
                     /locus_tag="PK125_00425"
     CDS             16928..18043
                     /locus_tag="PK125_00425"
                     /inference="COORDINATES: protein motif:HMM:NF040218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB36604.1"
                     /translation="MPLFVLIFFCAISLILPSEGDCAQATFTWDKNTETYVAGYKIYY
                     GNSSGLYNWSIDVGNVTKYTITGLAEGSTYYFAATAYDTSKKESKHSSEVSFNSCTYT
                     ISPTTAKFDPQGGTGTVRISTQKSCNWTATSGVPWITINSSNKGTGSGEVSYSVTTNS
                     KNSSRSASFTIGHSIFTVNQAGHTQISKDASSIKEYMVKVTIKQKNKGSGVVKSYDRK
                     IDCGDVCFSNYKQKSTVTLYATPGSGSTFIGWAPATLGCEGTGPCMVSVDKIKKIQAI
                     FAGDYELKLVTVSKNGGAGRVTSSPWALDCKSGNDGKCDGTYRYNRDVTLTSYASPGS
                     IFVGWRPEKLCPGTGKCTIAMDRKRIIKAVFSKPIKD"
     gene            complement(18906..20333)
                     /gene="gatB"
                     /locus_tag="PK125_00430"
     CDS             complement(18906..20333)
                     /gene="gatB"
                     /locus_tag="PK125_00430"
                     /inference="COORDINATES: protein motif:HMM:TIGR00133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HPB36605.1"
                     /translation="MRYEAVIGLEIHVQLNTITKLFCDCPNSPGDEPNKNTCPTCLWL
                     PGALPRLSADALEKATLACLALNCTIQPESAFDQKVYYYPDLPKGYQLSQHHKPLARN
                     GWIDIIGEDGELKRLRIHHVHMEEDVARLVHETEGKTPISLVDFNRAGAPLIEIVSEP
                     DLRSPHDAIEYVKALRTQLRYTGSAECSMEQGTLRVDANISMRPVGSDAFNTKVEVKN
                     MNSIRNVAEAIAYEIKRQTESIEKGEPIVLHTRLWDPDKRVTLPMREKFSGPCVPDPS
                     VPRIVVTPDWLDKIQMRLPEMPVQKANRFLKDYGLTPEEAAGMSQERDMSEFFEEVVR
                     KGLPARRVASWIVTHLMPALRDRNQTIRETTITTNRFTELLTMLESGVINAHAAKEVL
                     LQLLSDDQSPEEIVEKGSLRQVSDTAELEGLIDKILADHPSDVEEYRQGNAKIMGFFM
                     GLAMKASQGKANPKLLKEILTKRLG"
     gene            complement(20339..21553)
                     /locus_tag="PK125_00435"
     CDS             complement(20339..21553)
                     /locus_tag="PK125_00435"
                     /inference="COORDINATES: protein motif:HMM:NF013584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HPB36606.1"
                     /translation="MTCHIYTHKETSHISSPREGPLTGLKIVVQPNMSVTGWPTNAGS
                     RALNGYVALEDATVVKRLRSGGATLVGSARMSELGFGLGGDTLAEACSGDEVTIGLIT
                     DTMGEARIASAVSGMFGYKPSFGIISRLGMIGLIPSMESCGIVGRHLENVIAVAEVIA
                     GFDENDFSMADEPLPDLGESMKSLRIPSTVGVIEECHRMLNESEKNAFKAGITKLKDK
                     GFKIKELSFENFELFRAVHNVIGSVEASSSSGKYDGVRYGHRAASDKNWNDMYLKTRG
                     ESFGTLIKSYLFQGAYFQFENYPSFEDACRIRGGLVRKMSRLLETVDVIALPTRRLLF
                     DAMNATTIAETYEAFSFTVPANVTGQPSLSIPRFASDSTGDVGLQILGEHKSDVRLLA
                     LGMELSSIVKGV"
     gene            complement(21564..23702)
                     /gene="aspS"
                     /locus_tag="PK125_00440"
     CDS             complement(21564..23702)
                     /gene="aspS"
                     /locus_tag="PK125_00440"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: protein motif:HMM:NF001750.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HPB36607.1"
                     /translation="MRKDGAHMSYRDRIHCGQLNISDDGRVVTLAGWVDALRDHGEVL
                     FVHLRDRSGIVQVVFGPESTDQTVCEKAGLLRNEFCVLISGRVVKRQEGTENPNIATG
                     NVEVKADSLTVLSKSGVLPFPISEKSMVAGMALKRDEPVAEDLRLQFRYLDLRRPTIQ
                     DHLIKRHRIVKTVRDYLDEQGFVEIETPILTKSTPEGARDYLVPSRHHPKNFYALPQS
                     PQLFKQLLMVGGMERYFQIARCFRDEDLRPNRQPEFTQLDLEASFIDEEFIYALFEEL
                     SARMFEVGGIKLPRPYPRMTWLDAMNATGSDRPDLRFDMTFRDCTDIFSDTKYGIFKQ
                     ILERGGCIKGINVKGQSEKLSKNVLQNEYAKEIVPGFGAKGMTWMRVLDGGLESNIVQ
                     FFSESEQSEILTRFTAQKGDVILMIADPSWYLVCSALGQLRLHIADRLGLIPSGMFCP
                     LWVTDFPLFEATDDGVTSSHHPFTMPDRTDFDPKNIEELLSLRSRAYDLVVNGEELGG
                     GSIRINDRDLQNKIFSALGLSHVDVEEKFGFFLRALEYGAPPHGGIALGVDRVVAMIL
                     GTTSIREVIAFPKNRSAYCPLTQAPSQVSSVQLAELGLLDLGKDQSLPGAEKQQDLID
                     SLSWVSRIKINNDERAVIMASVKDAENLAAIVSERKEDGEPLFSVVAPMNRTRKGKEA
                     RISQLSERGDLFKNAPAMKGRYFKVASILE"
     gene            23840..26020
                     /gene="rnr"
                     /locus_tag="PK125_00445"
     CDS             23840..26020
                     /gene="rnr"
                     /locus_tag="PK125_00445"
                     /inference="COORDINATES: protein motif:HMM:TIGR02063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008997 - ribonuclease R activity
                     [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HPB36608.1"
                     /translation="MGAMDDGREKCDKIIRVMRKKKNHQDLTRDIVLHVFKQVKRPIA
                     QNELVAILSLNKKQGKALKHILRELVRDGSVLQLKNKRYGLPQEMNLIAGTLWGTRSG
                     SGFVVPDNVNEKDLFIPARFIKNAFHGDKVITRVERNLRGRREGTIIKIIGRKIKNIV
                     GFVRHHRDLMYIIPEDERISHHFIVTKPTKYSGLKDDHLVAARITRFPDGGIDPTCTI
                     LKAFAGLNDVHSIIQFIQYKNSLPFRFKKRTENEAQALDLSIDRQDRLDLRETIHVTI
                     DGELAKDFDDAVCVEKNDHGFTLYVSIADVSHYVLPGTDLDREAYERGTSVYFPGSVI
                     PMLPKILSNDICSLNPNMDRFAITAKLIFNRNGDITSSSFHKSIIRSFLRLTYNEVED
                     ALVRKDRAVRKEIQKILPALEYMGELAALLHTKRERLGSLDFDLPELNIILDIKGGLR
                     NILKTERLFSHRIIEEFMIVTNEAVAKFLTENKVNTIYRIHEPPDAEKLRDFERLLQT
                     LSIKYKRDNKNIRDLQRTLMSLEGTDYEFLVSRILLRSMKQARYSSQNKGHFGLASRC
                     YLHFTSPIRRYPDIVCHRALKNILSGNGKRYGETELETMANYLSNRERTAMEAEREIG
                     DRMRVLFMKDKIGQEYDGVISRVVSFGFFVELADVFVEGLVLVNTLYDDYYHYEEERF
                     RLIGRRMRNIYRLGDRVKIRITLANVEKNQLHFSLVKKISDSNR"
     gene            complement(26174..26416)
                     /locus_tag="PK125_00450"
     CDS             complement(26174..26416)
                     /locus_tag="PK125_00450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB36609.1"
                     /translation="MTREEKKLVTAHMDQVFHGQTVRQALPVCECGKYYDEKNITEAP
                     AVYFREIDVFGKTFTLIEPLCPVCKQRIHASFSILN"
     gene            26742..28409
                     /locus_tag="PK125_00455"
     CDS             26742..28409
                     /locus_tag="PK125_00455"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: protein motif:HMM:NF013461.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004612 - phosphoenolpyruvate
                     carboxykinase (ATP) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HPB36610.1"
                     /translation="MASIHGIPKSPWRAIIESAFFASSVRKTSMAELYALAKKQPEVM
                     VTSEPMYRPEQFDLPHDAKVLVSNDGGIFGRTARARRLVRTFTKADKDKYQSMLSEAT
                     YHLNRQPGLWLEGMIGLHPDFMIKAHLLSPETDAKNMLDWGINFIPFMPPWSDLYKES
                     RQLGEPDILVLAYPEWSNPEFKDGLVIIDEDQNCIAILGLRYFGERKKGTLTIAWTVG
                     VRHNMVACHGGIKKIGDKPPVAVFGLSGSGKSSITNSSDHEGTLTPEEKVTVIHDDAF
                     LIDLDHNLSIVLEPSLFDKTDAMEFDDPVVKYFYSAQNMAVTGMADGTRRLVGKDIRN
                     DNGRCLKSRDMFNHANFCEKPSTVIWLQKDTSLPPICKVTRSAMAVAMGASLSTLRAK
                     GVENADPRDFERLVIEPFANPFRVHPLVEDCRQFLKLFKSGCECYVMNTHAVGMPGHL
                     TDIPKTISLSIVTALVRGKIEWKDWRAFSGLQIPKNGNELFGPDYEKKYSPPTRHPGY
                     LRFLRDRMQDRITFLSNKRDLENDMESIFIDPLVASRTVLDHILEPL"
     gene            complement(28578..29171)
                     /locus_tag="PK125_00460"
     CDS             complement(28578..29171)
                     /locus_tag="PK125_00460"
                     /inference="COORDINATES: protein motif:HMM:TIGR00413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HPB36611.1"
                     /translation="MKGTYYVVKGTYEITAGTTKVVYHIGKFTYKAVKAPMEWAFVNE
                     DIETIDGLPPKEALKQGRVRNSPYVVKGKTYYPMSIEKAKTYEEIGVASWYGYETLRT
                     KGGRMTANGEVFDPRQFTAAHKYLPLPSHVMVTNLENDQWVIVRVNDRGPFPSDYNPS
                     SGDRIIDVSEGAAKRLGFHKKGLARVKVEAIELKEER"
     gene            complement(29270..30097)
                     /locus_tag="PK125_00465"
     CDS             complement(29270..30097)
                     /locus_tag="PK125_00465"
                     /inference="COORDINATES: protein motif:HMM:NF012451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HPB36612.1"
                     /translation="MRQKDYYRILGIEKDATHQQVKETYRRLAFEFHPDRNKGDLNAV
                     EKMKELNEAYAVLSDPEKRNRYDSMNQQYGYDGYDHFRKGYSEQDIFRGSDINQIFEE
                     MTRQFGFRNFNDLFREFYGPGYKTFEFKHSGIFGKGFIFTGFPFGRHGNQQNTFSSSK
                     TAFVSGLLAKLAGYMVRKAFKGRYTSQQTADRYGIITISREQASRGGKVSYIDPTTSR
                     QLVVTVPVGVREGQTIRLKGAAGFYGDRSQADLYLRVQFRKSLLESVKKLYNKFLSK"
     gene            complement(30107..31546)
                     /locus_tag="PK125_00470"
     CDS             complement(30107..31546)
                     /locus_tag="PK125_00470"
                     /inference="COORDINATES: protein motif:HMM:NF014443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter TrkG"
                     /protein_id="HPB36613.1"
                     /translation="MKRKNIVYVLSRLWIIAGMFFILPLCFSVYYKDGIHYHFILIAI
                     GILILSGLVWPFGSKPKELTTKEGFLLVNLCWISFTFFGCLPFLTTGSIESLTDAFFE
                     SMSGFTTTGATILRDIEILPKSLLLWRNMTQWLGGMGVIALAVAIFPFTGMGGSHLFK
                     AEVPGPSKDKVSSRIRDTAKLLWGVYLIFTIAQTIMLMLAGANFFDSLCITFGTMATG
                     GFAPLNASIGHFSSPYVHYITILFMTLAGINFNLHYWALRGKPVHYFKNPELRLFLSI
                     IMCSTIFIVLIRFFAGTELSEETVRDSLFQTVSLITTTGFITKDYELWPAATQIVLFL
                     LMFAGGCASSTGGAIKSVRILVLFKYIGGEVKKLFYPRGVFPIKLEGKLVPENLVSNI
                     MAFVMLYILFFIFGTIVVSSLGMDILSAAGAVATTLGNVGPGLGTVGPVDNFADVPVL
                     AKWILSFLMLAGRLEIFTIVVLFTGRFWK"
     gene            complement(31562..32905)
                     /gene="trkA"
                     /locus_tag="PK125_00475"
     CDS             complement(31562..32905)
                     /gene="trkA"
                     /locus_tag="PK125_00475"
                     /inference="COORDINATES: protein motif:HMM:NF007039.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HPB36614.1"
                     /translation="MKIVIIGAGDVGFNLAKRLTHEGHDLILIEENPEKCNRASEILD
                     VSVIQGSGTDQKVLRAANLGSADMLIAAAGVDEVNILACLFASKLGVRRKIARVRTSA
                     YYRSDSILKADDLGIDLFIHPEEEVSEEIVRLLMRSTASEIIEFEGGKILMLGMKVDP
                     GFPYLNKQLKDMGTAEQRRHFRILAVLKGDKTIIPTGEDYINKNDQVFVVTGKDYLPE
                     LLKIAGKADEKLEKIMILGGGKIGRKVAQSLEKRGIDVIIVESDKAKSLKIAGQLEKT
                     MVMHADGAEIDSIVREGILGMDALVAVTSDDENNMIACLLAKHLGIRKTIALVNKVNY
                     LPLMPVIGIDSTVNTQISTVNAIFRLIRRGGIVSVATFHGIDAEAIEVEVKANDRLTG
                     KPLKKLKLPDDALVAAIVRGTEVFVPYGDTIINPGDKIVVFALPHAIKAIESRLS"
     gene            complement(34726..35661)
                     /gene="dusB"
                     /locus_tag="PK125_00480"
     CDS             complement(34726..35661)
                     /gene="dusB"
                     /locus_tag="PK125_00480"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HPB36615.1"
                     /translation="MLAPISGVSDLPFRMINRSFGAPFAFTEMIDVKAVSVFDKRTKR
                     MLASNAEDRPLGVQLLAIDSKDIDRALKSLDHIEYDLLDLNAACPTPKVVRKGKGASL
                     LKEPRKLKKILETLVRHTKTPVTVKIRSGWDIDSVNARDVALCAEDAGISALFIHGRT
                     KMQGYSGTVDYDTIKKVKDALKIPVIASGDNVNVSQVKRMFDITGCDGVAIARGSFGN
                     PWIFSNLIQFFRDGSIGDMPSVSIRVDVMNHHLNLLVRHYGEQRALSIFHKFFIWYTK
                     GIPKTRVLRDRAFRTEKMDDMVSLMEELQKLDERK"
     gene            complement(35786..37153)
                     /locus_tag="PK125_00485"
     CDS             complement(35786..37153)
                     /locus_tag="PK125_00485"
                     /inference="COORDINATES: protein motif:HMM:NF020252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HPB36616.1"
                     /translation="MVQELNKKSSPTFSSSTTIEGFTEKETIALFQAGKIRSVENGEY
                     VLRKDETCNILTFILDGSSKILFSENDGESFSVSIKKGSLFGDPDLSKDKKNRFSVVS
                     SGASRILEIDQFAFNLLPPGIQISVYRKILDTFTELNDELVTKSGALSKKIESLVSYV
                     KDFYNDNDQYARSIMIQDMIKNFPRLPIQINRLTSMLLDEGASVAEIAGFAKMDPSIV
                     SIVLKIVNSGYYSFQRKISDFHHAFVLLGFNQVYQILMENFLQGVMPRHFNLKALNLH
                     SAVISQIAFDIAQLSKKSKPVMMSTLGILHDLGKCVILVSKAKKPELDIVMARLDDCK
                     VGSLLLDAWNTPVIIGQSVEYQKYPNFCSPEYIPENCIENVAILYIAHLCFDYLSGNE
                     KAEFSNTYLQEYLNIVNLHNHSIESLMSQHIMPSLIKKMDVFPDYVRDFIKNQQTTVT
                     FSSNK"
     gene            37570..38325
                     /locus_tag="PK125_00490"
     CDS             37570..38325
                     /locus_tag="PK125_00490"
                     /inference="COORDINATES: protein motif:HMM:NF012596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HPB36617.1"
                     /translation="MDVVTELFDTGVYTITLNRPDKKNAMDSALLQGLNDALKRADTK
                     SPQIIVIRGSGRAFCSGGDLIEFNESPDPGKQIDGMAECLHQSIMLIRNIGAIVIAVV
                     EGVAVGAGLGLSLACDLTLASKNTIMNLGYRRIGLTPDGGGSISLPRLIGAKRFNELY
                     LFSANVNMSEALMLGLVNFVCDENEIEVKLAEIVKNLLSLPMETISRFKTLVNNSMFS
                     GLEGHLDMERQYVKELGAQPLFKERIASFFKRK"
     gene            38478..39836
                     /locus_tag="PK125_00495"
     CDS             38478..39836
                     /locus_tag="PK125_00495"
                     /inference="COORDINATES: protein motif:HMM:NF012686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB36618.1"
                     /translation="MALIYLFTIVGYITLLWVTGAFVTRKSGSSEAYLVASRGLSVTF
                     ISVLIAGTWIGGVSIVGMAQGAYIYGISALWFQAGIWIAMCVTALLLNKIMKGNKTYS
                     ILDVVGNLYDTRTAKFAGILQLIFSIWVVTMQVVGGGAILAVILNGQISFQSGMLLTA
                     IVFTLYNVMGGFVATAYTNLIHIGAIVFGIFLGGFYVMLNAGTISPMAHQSHYFTPFG
                     DLGFFQAVSWAFINFTLGVLAQPVINTASSARTSKEGKQGILIGNAIAIPVVVMAALC
                     GIVAKNVFPHIPSISALPALLDIVPPYVAVFFLISMWAPLMSSGSPFLMGATTLAVKG
                     YIAPLLKIKDDRQLLLISRLTTLIIGIISLLLGFFVKQILREVTWIAVFLSAIVYIVF
                     IGWFGIRVDSRYAFISLIGTLLIVLCSFLFDLHKIVHPVWPVTLYVFLVMGSGALVSK
                     KH"
     gene            39942..40409
                     /locus_tag="PK125_00500"
     CDS             39942..40409
                     /locus_tag="PK125_00500"
                     /inference="COORDINATES: protein motif:HMM:NF014912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin family protein"
                     /protein_id="HPB36619.1"
                     /translation="MSVKNLSALLDMAINKEIEAQEFYKDLESRVSDTEAKQTLQFMA
                     KEEKKHEEFLVKYKEAGLGPETLKQSQIIDYKIAEHLEKPDISAKMDSKDVYLVAAHR
                     ELNSYNFYKELAEIHPKGSIRDMLLKMASEEMKHKEKVEYLYSNTAFPQTAGG"
     gene            complement(40521..41339)
                     /locus_tag="PK125_00505"
     CDS             complement(40521..41339)
                     /locus_tag="PK125_00505"
                     /inference="COORDINATES: protein motif:HMM:NF013397.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015499 - formate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015724 - formate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HPB36620.1"
                     /translation="MANKPVEIVQLAGDAGKYKANLTPGNYLVRSFMAGLFIAVGGAL
                     ATVCSTGTTIPGIKSLISGSVFPVGLITIVLTGMSLFTGDTMLIPMAVFQKKSTWSKV
                     FNAWFWVYIGNFIGSLFWAYLMSVGPFSKGGGPEITVFGQNAIAIAEAKTLPYMAAGG
                     MGLWSSFMKGIACNLLVNVAILLAISSKNMIGKFFGVWFPIMAFVSSGFEHSVANMYF
                     IPAGIFVGANVTWGQFIYWNLIPVTIGNIVGGFIFIGAVYYWSFKKELSSIAPT"
     gene            complement(41374..44079)
                     /gene="fdhF"
                     /locus_tag="PK125_00510"
     CDS             complement(41374..44079)
                     /gene="fdhF"
                     /locus_tag="PK125_00510"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013279222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:complement(43015..43017),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
                     /protein_id="HPB36621.1"
                     /translation="MDSDLIKFSIDGRVVKGRRGQTILEVATENGIYIPTLCYHPKIS
                     KTGACRICIVRVNDRMLRTACTEPIMDDMTVVTEDEGIIANRKFVLELLLMEGDHNCL
                     YCESNGDCELQTLVQKYQVGPVTDTSIRHDREIDFTSSNAIKRNEMRCILCQRCIKTC
                     NEIQMCRVWGTCERGSETHLIADDNKRIGESSCVKCGQCVQMCPTGALSFQTVLGTGQ
                     NWELTKETSICIYCGVGCQIDFYKNKEGVLVKAIGNDDGPNRGHICVKGRFGWDFVQS
                     PKRLTKPLIKKNGNFVEASWDEALNLVAEKLSDVKKKSGADAIGTLCSAKCTNEENYL
                     MQKFTRAVIGTNNTDHCARLUHSSTVAGLAAVFGSGAMTNSIREIPTSSGFLVIGSNT
                     TENHPVIGSMIKKEVLNNKKKLIVVDPRNIELSKYADYYFAINPGTNIAILNGFMHVI
                     VKEGLYDNEYVEKRCEGFADLAKSLEKYTPDYVAKICGLNNPDDIVAAAHLMATLKPM
                     ALYYSMGITQFVSGVNGVKSTANLQMLLGNVGVAGGGVNPLRGQNNVQGACDMGGLPN
                     VYPAYQPVTTEESKSKFEMAWGTSNLSLKPGLTLMEMFNHALAGDFKALWIMGENPVI
                     SDPNQQHVIDALKALEFVVVQDIFLTETAELAHVVLPACAFLEKSGTVSNTERRVQMM
                     NKVVSPPGQAKDDWWIITEVAKRMGVNWNYKKPKDIFAEIRKVTPSYAGMTYERIRKE
                     LLQWPCPTEDHPGTQFLHRDRFSRGLGVLTPIEYTSPAEETDAEYPLIMTTGRLLDHF
                     HTGTMSRNSKVLNENVPEGYVELNPNDAQNYNIKDGETISISSRRGSLRVKTKVTEKT
                     KPNVVFFPWHFFEAAANKLTNDALDPLCKIPEYKVCSCRIEKVYP"
     gene            complement(44421..45590)
                     /locus_tag="PK125_00515"
     CDS             complement(44421..45590)
                     /locus_tag="PK125_00515"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HPB36622.1"
                     /translation="MKPSLSDPRKYMEVVEAQSAKRTGRVCGDYVVTERSPEATLTVV
                     ADGIGTGIKARVAAVMCASRLVELTRSGFTLREACSKIVHTMHEARTKDIPFSAFSAC
                     RVLNNGHATIMAYEIPSPILINNRLAAYLPKQRFYPLGLEMIAEVNCMLNYGDGVFLV
                     SDGVSQAGLGHQYRMGWSVQGACDFINGCLAQGYELKMIPNKVLEKVKEISGATYGDD
                     TTCVAIFCREGKTLNILTGPPADKNNDKKIVEDFLSMKGTKVICGSTTSEMVAKILGV
                     PIDMKTIPSAYHKPPSYEIKGIDYATEGAICLNQVHNILEENPDKLETDSSVSDLYRL
                     FHSADTINFVVGTASNPGHENIVFRQMGIFPREVIVKLLADKLKKIGKLVNLTYV"
     gene            complement(45571..47301)
                     /locus_tag="PK125_00520"
     CDS             complement(45571..47301)
                     /locus_tag="PK125_00520"
                     /inference="COORDINATES: protein motif:HMM:NF014904.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-Fe] hydrogenase large subunit C-terminal
                     domain-containing protein"
                     /protein_id="HPB36623.1"
                     /translation="MHDIDNRQIVFTLTARCRDCYRCLRNCPVKAIRMQNGQAYVDET
                     RCIACGTCIRECPQQAKTFRYDINTAQKLIESGAFVSASIAPSFAAVFSGWQGMRLPA
                     ALRALGFRFIGQTSHGAYQVSAHSSKVIEKDGSKPYIATACPALVNYIEKYQQELVDN
                     LLPILSPMATHGRMLKDRFGMESKVIFIGPCVAKKTELYRSDVVDAIDCVLTFKELMV
                     WLEQRGIDLSNCEESNFDEKPVRFAQLYPLPGGLIKTAGLKDDGLDTKLVRVDGFQNV
                     KEIFHNIPKESSYTLIEPLFCSQGCINGPGISSIKNVFERRKDIIQYDRSIAGNDVVP
                     EIYDEKIVKASYKKEEKIGKEVGEEEIQGILERTGKSDLQQQLNCGACGYESCREKAI
                     AVAQGMAEPEMCIPYMRRLAERRTDQLFNTTPNGILMLDLELNIIGINPAFKKYFSCS
                     DAILGRHISYLMDPAPFEKLITGAAESLDITVFHRPYNLLCRELLYILKQEQQIVGVF
                     VNITSEEEQVKKLREMKSQTVAQAHELLEHQIQMAQQMALFLGESTARGEELVRKLMS
                     LSEGDETKSE"
     gene            complement(47294..49321)
                     /locus_tag="PK125_00525"
     CDS             complement(47294..49321)
                     /locus_tag="PK125_00525"
                     /inference="COORDINATES: protein motif:HMM:NF040763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent [FeFe] hydrogenase, group A6"
                     /protein_id="HPB36624.1"
                     /translation="MSEERYVTIDNKTILIEEEGNLLELVRKANIELPTFCYHSELSV
                     YGSCRLCMVEIEGLGLVPACSTPPAMGMKVRTNTEEIRKMRKIIVELLLANHNQDCPT
                     CQKGSTCQLQALARRLGVSKVRFKKQNRELPLDDSSPSLVRDPNKCVLCGDCVRICSE
                     VQSVGAIDFAYRGAHTVVIPSFGKELDKVECINCGQCARVCPTGALMPKSEIEEVWKA
                     IYDPNKVVVGQVAPAVRVALGEMFGLEPGVTTTGQIAAALKAIGFDKIFDTSFSADLT
                     VIEESNEFINRIQKNENIPQFTSCCPAWVKFVEQYYPEFVNHLSTCRSPQQMFGALAK
                     EILANDLGIGPEDVVVVSIMPCTAKKFEAKRPEFNHNGMRDVDNVLTTQELGRMIEEA
                     GINFRELEPESFHLPFGFKTGAGVIFGNSGGVSEAALRYITEKITGEKRDSYEFTTVR
                     GDNGMREATLTVGSKDFSLAVVNGLGNARNLLERIKSGDVHYDFVEVMACPGGCVGGA
                     GQPVYSNPSIRHKRTKGIYENDRMLELHKSQDNPYVTELYSSYLGDIGGEKAHHLLHT
                     SYKTRKRISDEDMTFSSGDDQGFEVNVCFGTGCFLKGSQKLLRDILEYIRSNGLHSSV
                     KVGASFCFETCDRGPVVKIGDKIIEHCTLDKAREAIEGMAASTLKEVQRNA"
     gene            complement(49318..51222)
                     /locus_tag="PK125_00530"
     CDS             complement(49318..51222)
                     /locus_tag="PK125_00530"
                     /inference="COORDINATES: protein motif:HMM:NF013662.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-ubiquinone oxidoreductase-F iron-sulfur
                     binding region domain-containing protein"
                     /protein_id="HPB36625.1"
                     /translation="MNLTLMGLNKIKEDYLAAQTVLARRIIVCAGTGCIVNGSMKVYE
                     EFVRCIKDSGLNAVVELKKEKEEDGVFVSKSGCQGFCQIGPLVTILPENILYTKVRPD
                     DVQDIVETTLIRGELLNRILYVDPDTKKPCKNQSEIPFYRKQQRFVLANCGVIDPEDL
                     REYVAHGGYVSAAKACLEMTPLEVCKRIGDAGVRGRGGGGFPTGKKWEMALQKESDKK
                     YVICNGDEGDPGAFMDMGVMEGNPHSVVEGLIIAARAIGADEGYVYVRAEYPLAVKRI
                     RKAIQDAYKAGMLGRQIFGSSYNFDLHVMEGAGAFVCGEETAMIASIEGRRGMPSPKP
                     PFPTQRGLWGKPTVINNVETLSNIPLVLAAGLDQYRSLGTHESPGTKTFAVTGHVANT
                     GLIEMPFGSTLREIVFHVAGGMTSDDGKPMNDGFKAVQIGGPSGGCLTRDHLDLPLDF
                     DSLKQVGAMIGSGGLVVMNKNTCMVSVARFFMRFTQEESCGKCVLCREGTKQMLSLLD
                     DIIEGRATEETLTILEDLAIAVQKGSLCGLGKTAPNPVLSTLRYFRDEYEAHVKHKYC
                     PTGKCKKLTKFWIDENLCRGCTLCVTKCAIGAIIGEKGQPHKIDQMLCRKCGACIEVC
                     KFQAIKGGKS"
     gene            complement(51197..51703)
                     /gene="nuoE"
                     /locus_tag="PK125_00535"
     CDS             complement(51197..51703)
                     /gene="nuoE"
                     /locus_tag="PK125_00535"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF005722.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HPB36626.1"
                     /translation="METLRKFEKVCEIVDQNDRSAARLIPILQAVQSEYRYLPEEILT
                     YIATSLGISPASVFGVATFYSLFTMKPKGKYIIRICDGTACHVKGSMNLYEALRNKLT
                     LKEGADSTPDMLFTLETVNCLGACGLAPAMVINDEVFGQLTPERVDSIIDDILDKEEK
                     NEFDTHGA"
     gene            complement(51717..52388)
                     /locus_tag="PK125_00540"
     CDS             complement(51717..52388)
                     /locus_tag="PK125_00540"
                     /inference="COORDINATES: protein motif:HMM:NF003994.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HPB36627.1"
                     /translation="MNGSNHKIKMALKAIPEPTLRRLPIYYQYLKQIQAEKKHEHISC
                     TQIGNELNILPIQVRKDLAITEASGKPKLGYRVKHLLSNIEDFLGWNNTTDAYLVGVG
                     HLGSALLGYQGFKEYGLNIIAGFDSDEAKTGIEIHGKMVFPVKKLPDMIKRMGIKIGI
                     LTVPSYCAQDLANLMVDAGIHAIWNFSPVKIMVPAEIIVQHENLASSLVVLSKKLAVS
                     MRTDK"
     gene            52539..52913
                     /locus_tag="PK125_00545"
     CDS             52539..52913
                     /locus_tag="PK125_00545"
                     /inference="COORDINATES: protein motif:HMM:NF018753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DRTGG domain-containing protein"
                     /protein_id="HPB36628.1"
                     /translation="MKLFDVQKILEAEVLCGDDLLHKDVHNWFACDLINEMLLYVTPD
                     TLLLTSLTNIHIVHTAQVMDAIGVVFVGGKRPAESVITNSKLYNIPLLTTKHLTFECC
                     GRLFLNNLHDPTVVKDATNAYR"
     gene            52900..53370
                     /locus_tag="PK125_00550"
     CDS             52900..53370
                     /locus_tag="PK125_00550"
                     /inference="COORDINATES: protein motif:HMM:NF024970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPB36629.1"
                     /translation="MPTDNLQFTPHIKLEFKINEKDFFVAGESASRVKKTLQQLGLHQ
                     DIIKKVAIIIYEAAMNVAIHGNHGMLRIYIDPESILVETDDEGDGIPDIELAMQEGYS
                     TATHEIREMGFGAGMGLFNIDQCSDELHIDSKVGTGTKLQAKVYLRKKDSISDP"
     gene            53383..54723
                     /locus_tag="PK125_00555"
     CDS             53383..54723
                     /locus_tag="PK125_00555"
                     /inference="COORDINATES: protein motif:HMM:NF014904.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-Fe] hydrogenase large subunit C-terminal
                     domain-containing protein"
                     /protein_id="HPB36630.1"
                     /translation="MIRNFHSVRLDPEKCKGCTNCIKRCPTEAIRVHDGKATIIEERC
                     IDCGECIRACPNHAKFAVTDELDKLAAYKYNIALPAPSFFGQFKNQEHIEDILHAFLR
                     LGFDDVFEVALAAEIVADIVHRHLLNDRVKKPLFSSACPAVLRLMQIKFPDLLHQCAP
                     VLTPMEVAARLAKEEAVKKTGFSYEEIGTFFISPCPAKVTEVKHPIATKSSAVDGVIG
                     ANIIFKDIRKGIFRPDGISRSSKLQRATSLGMAWGFVTGESKNVNSGRALAVSGIHNV
                     ISLLDEVERGELQDVDFLELQACTGGCVGGPLNVTNLFVGRVRLRDLINRFGSKEPFF
                     NKNKLIEFYDHGQFDATEPFQPRFLMPLDEDVSQALIKMERLDQITNDLPGLDCGACG
                     SPSCRAFAEDVIRGIAIETDCVIKLKEKVKMLSDGILDLARVIPSASSDNPKQE"
BASE COUNT        15774 a        12127 c        11071 g        15765 t
ORIGIN      
        1 gatttgttgc cttttgcacg tgcacagcgt atgatatcct acaatgatga tgtgttaaag
       61 aataacttca gccactgcac aggagaatgt ttatgacaaa tagaaaaaat aatggaagaa
      121 taatgataat cggcgccggg ggtgttgcca ctgttgcagc gcataagtgt gcacagattc
      181 ctgaaatatt taaagaaata cttgttgcca gcagaacact ttcaaagtgt gaaacaatca
      241 aaagtaatat caagaaacgt tacaataagg atattgagat tgcgcaggtg gatgcagata
      301 acttgcacga acttgtaaca ctcatcaatg atttccagcc atacctcgtt cttaatcttg
      361 cattgcctta ccaggacctc catattatgg atggatgtat acagacgggc gtgcactata
      421 tagatacagc taactatgaa ccattggacg aagcccgctt cgaatacaga tggcaatggg
      481 cataccaaga aagatttaaa caacggggct taatggcaat tcttggttct gggtttgacc
      541 caggagtaac gaatgttttt gccgcgtacg cacagaaaca ccttttcgat gaaatccatt
      601 acctggatat cctggactgt aatgccggga cgcatggcca cgtatttgcc acgaacttta
      661 atcctgagat aaatatccgg gaagttaccc agatcgtccg gcattgggat aacggtaaat
      721 gggttgaaac acctgccatc atagacgaag gcagtgtcca tttcagcttt aattaccctg
      781 ttgtcggttc aaaagaaagc tacctactct accacgaaga acttgagtcg ttagtaatga
      841 atattaaggg attgaagaga gcaaggttct ggatgacatt ctctgaaaat tacctaactc
      901 atttgagggt gcttcagaat gtcggaatga ccaggataga tgaagttgag tatgaggggt
      961 gtaaaatcat tccggtaaaa ttcctcaggg ttttgttgcc tgagccagcc tccctaggca
     1021 caaaatacac tggcaagaca gtgataggaa atattatgac aggaaaaaag gatggaaaaa
     1081 aaataacgag gtatatatac aatatctgtg atcatgaaga ggcgttccag gaaacaggaa
     1141 cacaggcaat tgcatatact acaggtgttc ctgcaatgat cggggcaatg atggttctca
     1201 caggcgtttg gcaaggagca ggggtataca acatggaaca gcttgatcct gacaaattta
     1261 tggaagcact caataaatac ggattaccgt ggcaggtgat tgaacatgaa ccactgccgg
     1321 acaggttgta aaatcgataa cgtgccagga ctgtaatgat taggccagac aaaaaaataa
     1381 aaagaattat acagtcaatc ttgggaaatg atttattggc tgtcagggat tactacgcct
     1441 tgtcagagga tgctcggagg aaaatggtta cagaactggg cccaagcgta ctcaggcgtc
     1501 ttacccgctt aatcaaaaaa tgcaaaaaac aagtgtttgc ttctgaaaac aaaagactcc
     1561 ttaaaatagc tcagagtgat atagccaccc cggtttattt cattgatgag attctcattg
     1621 aagagaacat gaaagtgctc aggtatgtca aggaccgaac aggttgtaaa atcctgcatg
     1681 ccttaaaggc ttatgcttcc tttgctacat ttcccctaat gcgggagtat ctagatgggg
     1741 tgtgtgccag tggtcttcat gaagcgaggc tcggtttcga ggaattcaaa aaagaagtac
     1801 atacctttgc tgcagcatat agagataaag agatggatca gataatcagg tattctgatg
     1861 ttataatttt caattcattt tatcagctcc agaaatatgg gctcagagca aagaagaaag
     1921 gaatagaaat tggcttaaga gtaaatccag gacactctga agttctcaca gagatgtaca
     1981 acccatgtgc accctgttca agattaggga ttatcaacga tactttcgaa aaatattttt
     2041 ctgggtacag ggatattgtt gaggggctcc attttcatgc catgtgcgaa caggactcgg
     2101 acatacttga aaggatactc aactcatttg aaaagctcta tggtcaacat ataaaaaatt
     2161 tgaagtgggt caattttggg ggcggccatc acataacccg tgatgattat gatcttgaac
     2221 gtttaatcaa aatcataaac gggtttaagc agaaatatgg tgtacaggta tatcttgagc
     2281 ctggagaagc aagtgtgctt aatgctggtt ttttggcgtc atccatactg gatatagtaa
     2341 caaaccaaat ggatatcgct attgtggatg catcggcaga aacccatatg cctgatgtgc
     2401 tccttatgcc ttacagacct cacatcatca aatcgggcca atcaaaagaa aagaaataca
     2461 catacaggat tgcaggacta agctgcctgg caggcgacat tatgggagat tattctttca
     2521 ataaaccgct taaaagaggc gacaaactga tttttactga tatggccctc tatagcattg
     2581 taaaaaacac tacttttaat gggataaatc taccagacat agctgtaatt aaggatgata
     2641 aaagaataga ggttataaaa atattcgatt ataatgacta caagaaccgt caatcataat
     2701 tctagcacac tgtaaatttt tactttttct gaaggttttt ttgttgcaat gttgcggtgt
     2761 tcacagcttt cctgatatca gcattattcg gatcaattgt gaggtaacct tgccacaggg
     2821 caattgcctc gttcagattg ccttccctgt attcctgaaa tcccttcttt gaaagaatgg
     2881 atttacagtc attcagtttt ttattgatat ctgacctgtt aaatgagagc atgtggacaa
     2941 atgtcttaaa ggcggaatag tttttggcca ggatgctgta agttttacag gcagatgtgt
     3001 acttctcctt ttcatattct tgatctgcag cgtctttcat ctcatctaca ttccttgcat
     3061 aatcttttgt tagtgtttgg tcatatggat atttggcctg ttcagattta tagaagtcga
     3121 tcgttttttg ataatctccg gttacgtgag gcctcttaat cgggattgac tcctgggcag
     3181 atgtctgttt agagggttcc aggacaggtt tatgtgcagc tctatctacg ggtatgttgg
     3241 aagaagaggt gccggttttc atctgagcac aaccagtgaa aatcaatgct aaaaatatca
     3301 taacagtgaa aaaccgtctc gttgtatgaa aataattcat tatataactc tcccagcgac
     3361 tttctgtttt tcatgggcat agcctttatc aattagcccg acaaacactg caacaagatc
     3421 ggcacgtaat tgcgtgcccg ccactcggac aagttctctg agcgcaacat cctcagtgaa
     3481 cgcactcctg tatggcctgg tagaagtcat ggcatcaaat gtatcagcaa tggcaattat
     3541 tgatgcaaac agtggaatag tatcactatt tagcccgtca gggtagcctt tgccatcctg
     3601 ccgttcatgg tgatgtttca cagcagggat atactttcgt aatgatgggg ctttgctgag
     3661 aatcgatgca ccgtactcta cgtgtcttat catctcatcg tattcaaact gagttaattt
     3721 tccaggcttg agcagaatgg agtccggagt cttaattttt cccacatcat ggaagagaca
     3781 ggctacctca aggtcgtcga gttcttcttt ggaaagccca attgctctgc cgatcattac
     3841 cgaaaactgt gaaacccgcg ctgaatggcc atgagtatat gaatcccgtg cgtcaatagc
     3901 tgcagccaca actttcacaa tcgaaacgta tgcttcttcg aggtcacggg catatttgtt
     3961 tagtttactc tgctggtcag ctatcgtagt tgacatttca ttgaaattgc tggtcaattt
     4021 ccctaactca tcgtgggaat aaatcttgag gggggttttc gctgtacctt tcttcagctc
     4081 atcaacgcct gcagaaagct ctctaattgg ctttatgaga aaagatgcaa gaagtgcact
     4141 tgctataatt ccgagaacaa caataatgcc aaagactaca agtatccttg tgcgcacctt
     4201 tgcctgtgcc tcaagaagca cagacttgtt gattgcgagt atgacgcttc ccatattctt
     4261 tttcataaaa acaacagggc atgatatttc aaagatggag gcagatggcc gcatcaattc
     4321 ttttactgtc gttccatcta ttctcttagt aattatcctg ccctgggatt caggcataac
     4381 caccccaccc attacagctt cgctgtgagc aacaaccttc atgtctggag cgacgatagc
     4441 aacatactcc atgtcattat ttgttacttt tgccttgaac acaagattat ctaggctgag
     4501 aagatccttt gaaagaagac tataaccagc agacgctgca atacccttac ttaccgattc
     4561 tcctctctta acaatctcat tcaatatgta attattcatg atctggactg taattataca
     4621 aagggcaaac gaagtgcttg tcagtaaaat cacaacaaag aaagcaattt taaagcgcag
     4681 acttgcagaa cgaaaccgct tacgcatcaa atgcgatgtt gtctggattg gaccagacag
     4741 aaggcgagca tgagaaatca ttttttgtgt ggtgcgtcgg atatcaacag ccattatatt
     4801 ttcatcattt cctatttggc ggaaggctta cttgccccat atcatgaatt ttatcggtta
     4861 aatagttgat aactttattt gattgatgtt aattgaagta agcaaaatat ttgtattaaa
     4921 aagctgaaag gcacgagact gagaaatttg ctgctaattt aaattttcct gcactctgct
     4981 ttaactagct cagatccata tgttcgctat acacacaaaa tttacaacat tgttacaaac
     5041 agtcatactc ttccaatagg ctgattttat tgaatcagct ttgaaggaat gaggcatgtt
     5101 gagcagtagt gactcgtgat attggactca agagggtctg atgggataac aaaaaactta
     5161 tcccatatat tctgtatttg cgtatcgttt agaaactgct ctatatagcg gtcagacatt
     5221 ccaaattcct gttgaatcct gagaaaacgc acagcgctgc ttatatcttt aatcagattc
     5281 gcattaattc aggattagtc agcaaccata atataccgac gtgactgaat tgacatattt
     5341 ttaatacatc aaagttgcga aataccctcg ggtaaccttc ccctccacga agaatatcgt
     5401 atcaaccaag cacacttgaa atcctgaaaa aatatgagac tcgaaaaaat cctgcagcca
     5461 taaatcggaa catccattca ttagcgtgat agagttttca atttccattc tgttggcctg
     5521 accacatagt tccaatcttc gctatgaccg atgcatgcta ataattttct gtaaataact
     5581 caaagtaagc gcgactacat ctgcacaatg gacaaacctt aggagcattt ctgttctttt
     5641 tcacataacc acaggcacgg catttccact ttacgacact atcttttcga aaaatcttcc
     5701 ctgccttcat gttttgcaag aggatacgga atcttctttc atgttctgct tcgactgaag
     5761 taataaatcg aagtatttta gcaacatctg taaaaccttc ttcgtctgct attttcacaa
     5821 aatcaggata catgatactt ttttcataat gttccccgga aactgcagtc tgaagattgg
     5881 aaattgtatt gcccactcct ttcactggaa agatgacttt gggaagttcg atatctctca
     5941 tttgaataat atttagtatc tgttttgcat gttcatactc atgattggct gtctcaataa
     6001 aaatcgcagc aacctgctca taaccttctt cctttgcttt ctctgcaaaa aacgtataca
     6061 tgtttctgtt gttagactca ccatggtaag ctaccaataa gttcttttca gtttgcatcc
     6121 ccttcaggtc cattagtaaa ttctccctca ttaatgttag atttttaatc aatcttataa
     6181 acaatcactt caagtttata gcctaatttc acattatcag tctcagccat agatagattg
     6241 tgcttattat atttcagcgt tttagaaacg tctactccgt atttaatgtt ttgccaattc
     6301 tcgcaataag gttgacttat ttttaggtca tgctaatatt gataatgttc aacaaccaac
     6361 cttgttaaag gagtatttgc tatggatttg aaagaatgtg ccaactgtgg ttataagtct
     6421 gagacatcat ttaaagatga tatctgtcct gagtgcggaa aaacattctg gaaatgtgaa
     6481 aattgcggat atacccttac tgcttcatca ccaccggaag cgtgtccatc gtgcaagcaa
     6541 aaatgtctgt ttaaaaatat aacctgctac actccagagt gtggtggtcc aggcaatatt
     6601 gatagcagac tgtaacccgg ttacagtatc gaaagataac tttgcactgt taatgttact
     6661 gataaacgcg aaaacatata tcgctaactt gtccaagctt ttgttcatcg atgcatgctc
     6721 ctcggtataa caatagacca tttggtttct cacattcctg tccccatgta taccattttg
     6781 ctctgagtag agttctggag acattccgat agggaatcct tctcatctca gtatcgtctt
     6841 tggctcattt tttttgatat tcatgactct tcgtggtgct atttttcgac attctttttt
     6901 tcctgtatgc tttttgcctc ttcaattttg cctattattt tttttctctt tttcctatta
     6961 ctcctctaca attctattta tcattcacta atccgcaatg gctactggaa gcgttttctc
     7021 cctctcaagg gtagctgtct tgaactgaaa gtgacccggg cgagatcagg aggctacccg
     7081 agccaggttt ttcccgctct atgcagagaa tgccggaagt agatgaagcc ttgtaaaagg
     7141 tcttcgtctt aggagtctcc acacgacggt gtacagaagc cctctctcct ttattgggag
     7201 atcccgtgag cgcccagacc atctcaacta tagcaaaaag tgtggattcc gaggtaaagg
     7261 cattccacaa tcgatctctc cctgatcact atgtctatct tttccttgac ggcatcatct
     7321 taaagatcag aaccggttcg ggttgaagaa gaaggcaatc ctgacggcct acggcattgc
     7381 cagacaaggc aaaggggaac tcatcgactt taccctggtc aggcataagc gtgaatatgc
     7441 ctgggtggaa tttttcttga acctcctcat gagaggcctt gcaggaacca atcttcgtct
     7501 cgtcataacc gacggcaacc agggacttgt caatgccgtg gatctcacct atctgtgaat
     7561 gccacgtcag cagtgctggg ctcataaaat gtgaaatgca gcaacccatc tctcgacaaa
     7621 ggaccgggat gaatatatga aggaagcacg gaacatctat gatgaaactc acgaaaagaa
     7681 gccattgaac gctacttgct ctgggctaaa aagtggagat cccgaaggtc caaggccgct
     7741 gcctccatcg aaaagtatct ggagaagctc cttgccttct ttacatgtcc aatgcctctc
     7801 tggagaaaat tgagaaccac caatctcttt gaacgggcct tccgggaagt gcgcaggagg
     7861 accagaccca tgagctgctt taacaacacc tagagtattg tgaggattgt ttatgccgta
     7921 atgagtcact taaacgacca gtgagagaag aagcccttaa aagaatttac acaaagtgct
     7981 tgacgttacc agatttggtg atcaggcttg ttatgtcaga aacttcgtga taagatagaa
     8041 taaatttggg gtaatagata ctgtattgta gtttattatt gcacattaca agcaagaaag
     8101 gagatagacc agtcatggac gataggataa aagcgatatt taaatccatt tacgaaggtg
     8161 aagccaaggc tgcactacgg ttaaaactat tcgcaaagca ggctgatcgt gaggacttac
     8221 cccagatcgc tcagctgttt cgtgttattt ccttcagcga ggagatacac ggtgaacgag
     8281 cactcaagat gctaaaagaa atcaaaagta ccgaggagaa tctcaaagaa agttttgact
     8341 ctgagacaac agtggcagag gttgcttatg atcaattttt gaaggttgcc gcagatgttg
     8401 gcgacaatgg tgcctcaatg attttttcac aatccaggga cgtggaggat attcatgcta
     8461 agctgtataa aaaagctctg gatcatctga tgacagaaac acagacaacc tattacgtct
     8521 gccaggtttg tggttatgtg gctgacggta tgttaccgga tgaatgccct gtttgctcag
     8581 cgccacaaaa tcagtttaaa gaatttaaat agaatttgtt aaatactttg ccgttcttct
     8641 tgacagacgg agatcaatga gaatagacaa gcgattgaat cggcacagcc agaaaagttc
     8701 tcctaaacag gagcagtcaa tacagtaaga atagactttt ttctgcgtgt tatgaaaatc
     8761 cttgttgttc tatcaattgc gttatagaat ttaagtaatt acaaaatgaa gggaggtgtg
     8821 acgatgaatg atgtcatagg aattgcatgg ttcaaagacg agcctactta ccgaagggct
     8881 cttgacattt ttaccgatgc caggaatatg cctgctacgt atgaagactg gaaaactctc
     8941 gtcaaacgag agtgtgaaga aatcaaacgt atcggcaaca tagctcttcg tgtggatatc
     9001 aacccggaag cattcactga atggtgtaac actcatggat tcccaaccaa ctcccaggga
     9061 agaatagcgt ttgttaacca tatggaactt gaatatcaga agaccggcca aggaataatc
     9121 atcgattgag tcgttgaggt tggttggaca gatcacgtag gttatcttca gacctttgct
     9181 ctaatgtaaa taatggtgta tggaatcgga cttttcgttg gcagagtgtt gttataaggt
     9241 tattgccgta ccgaacttat cattattcaa aaactcaaaa ttaaataaaa atgaatttag
     9301 tgtaaaatga aacgattatg tagataatgc actttctatt tgtttttcag ctttggaact
     9361 gcacggtctg caaagatgta actgagtaac gtataaacga gttttgcagc gaggaaatca
     9421 ggtgccttgt cgtgtgacgg gcataactca accacatcaa aacccacaat tcgtttcgcc
     9481 attgaaacag cgcgaagaag tttggttacc tgataccacc ctaagccacc tggctcaggt
     9541 gtccctgtgg acggcataat gcccgggtca aatacatcaa gatcaatggt aatgtatacg
     9601 ttatctttta gttgccgaac cgttttctgt atccaggttt ctgaattgaa tatctcatgg
     9661 gcgaagaaca tcctttttct gtccatgcca tcacgttcca actgatccat gctccgtatt
     9721 cccacagaaa caatttcttt tgtatattcc ctggcacggg caatcacgca cgcatggtta
     9781 taccgtgaac cctcataggt gtcgcgggag tctgcatgag cgtccaagtg caaaatgctt
     9841 aaatttttat aatgccttgc gtaggactta ataacgccca cagataccga atgttctcct
     9901 ccaagagcaa ctacgaattt attatctata agatagcggg tgactgccag atcagttttt
     9961 tttatcaaca ctgccgagga tgctgcccgg atcggtcgtg cagtgtatat cccgttgtcc
    10021 aacaccactc cgtccgtttc aatgtcatag aattcgaggt attgtgatgc atcaataagg
    10081 gcctgaggcc ctttgtccgc ccctttcatc cacgtgcttg ttttatcaaa gggaataggc
    10141 agaacgacaa ttgatgctgc atgataattg gcctgtgtac tgagctgccc acagaatcca
    10201 acgaaatgct ccggcatttc actccaagat aaacacagca gatgctatta ccgaggtcca
    10261 aagaccgtcc ttgttccctt cagctgactg gcagaaatgc cttgttgtaa atatgtggcc
    10321 acttgccctg tagaattgct tgcgttcgtc ccatgcctgg ttaggatcaa aagggatgtc
    10381 cagtgttgta gcaagcatgg ttgctgccag atcttctgca tattctcctg atacgacagc
    10441 cttctcgccg aaagcatggt gttcagagag atacccgtag ctgtcatagt ttttgggcac
    10501 tgcaagacca atagctgctg aaaccaggcg gttcggttcg tttgtctcgt ttcgagccat
    10561 tacgcaatat gttatgtcac cgggtttaag aagtttcaag ccggcagtcc ttgatataat
    10621 tttacatttg ggcgggaata tgcttgaaac ataaacaaga ttacattttt caatccctgc
    10681 actccgtaat gcaacctcaa aagacgagag tttatcttta tgaactccaa cgcctttggt
    10741 aaaaaatatc ttttttggcg tcatcttcct accctcctaa cataattatt cttgtaatgt
    10801 tataacaaag tctatcttca ccccccaaat tcgactaaaa attcgggggt gaagataccc
    10861 atagaactaa tgccctattc tttctcctgt tctctatgtg atgttttttg tttgccttaa
    10921 aataaaaaca ttcacccttc ttcaatttaa atactttccc gccaagaata aggtcaatac
    10981 tgcctccgag tacaaggcca aactcctcac cttcatgtgg ttcttctatg ggagttttct
    11041 cgccaacact cagttccatc aaagcaggat ccataagtct gttctgcgca gcaggaatca
    11101 gaatttggaa tttgttcaca tcatcaatct ccatttccac tctatcttcc aatttgaaca
    11161 caattttctc atcaaaggct ccgtcgaaga atcgcgatgg cttttcatca agggcatcaa
    11221 ggataccgat aaggctctca acagacaatg aagtaaggtt gcgctcgacc tgggatataa
    11281 atcctttggt aagtcctgct cgtgtggcaa gctcctcttg tgtgagagcc tttgcctgcc
    11341 ttaacatttt tatccgttct cctacgcttg cgtcttcttt tttcataaca ataaaagctg
    11401 acagaaagtt tacattatta aacaaataaa atcaaattta aatcaagtaa atttttattt
    11461 aacttgttga ctcactatgc taaactagct atgaaaggtg ataagtgacc ggaaaacaag
    11521 caattattga gttttttgta aaaaataata tccatgatct cttttatctt cctggaattc
    11581 atacactttc actttcacag gccttttcag gtgagaacat taacgtttat gtaacccgtc
    11641 atgagtcgaa catggttttt atggctgatg gtttttctag ggtttcaggt aaaatcgggg
    11701 ttcttatcgt aacgccgggt cctggtttgg ctaacgtggt aacaggatgc atggaggcat
    11761 atggtgatga tgtacctctg cttgttatcc atatagacac cggacgagaa ggtattgata
    11821 aaggtatcct ccatggggtg gcagagcctg aaaccatggt ccgtcacgtg acaaaggaga
    11881 tattttacgt tcaaagtaca gatgacctga tcataaaact tgacagtgcc cgaaaagttg
    11941 ccatgacaga tcggaaagga ccggtcctta tctctatacc ctatactctt ctcgaaaaag
    12001 aggttgaaac aataagatcc agcgaagaga accctgtaca gatgcagcac attgatgact
    12061 ttgacggcct tgagaaggtt ctggcaggta agaaaaaacc tgttatcatt ggcggcaagg
    12121 cgcttatgtt cgaaaaggct cgtccattgt tggacaatat atgtctgcat ttttccattc
    12181 ctttcctcac tactacatca ggcaagggca tcctggatga agaaaacgtc tggtgcttcg
    12241 gaaatatagt gcaaaagggg attgcaaaaa aaattgtctc ttctgctgac ataattattg
    12301 caattggtac aagacttcgc gacgtggatg caaaaaggcg aggagtaaaa attagtgacc
    12361 tcattcacgt tgacgtagac agggcctggt ttgacaagaa ttactcaacc gtatacagag
    12421 cttcaggaaa aatccttcca tttcttcatg ctttaaacga aattttcacc agcagctcct
    12481 ttaactggag gttaaaggag ctgaaagaag cgcaacatct ggaaagactt ggattacaaa
    12541 acaaattcct tgggtttcaa ctcataaacg tcatcaagaa ttcaattcct gacgatacaa
    12601 caactgtttg ggatctaaat cttatggcgt actgggccga gtattactat cctgttcgtc
    12661 accagcacgc ctttattatg cctcggggca tatcctccat attctatgcc ctacccgctg
    12721 ccataggcgc caaaacagca aaaccagaaa aaccatgtct tgcgatttgt ggtgatggag
    12781 gtgtcttgcc cacaatcagc gaactatcaa ccattcgaaa gtacaacatc ccggttgcca
    12841 ttctcgtatt caacaatagc ggcttcggta tacttgaaga ttacatgagg gctacttatt
    12901 caattgaagg gatgatggct cttgagaatc cggatttcat gaaaattgcc gctgctttcg
    12961 gcataaaaag caaacgggtt aaaactttgc aacaactgga aagtacctta cgccaattgg
    13021 tacaatggaa cgaacccttc attattgaat tttccagtcc tgtcttcgca ccaccatgga
    13081 ggacatagat ggcacaaatc ccattcctgc ccggctattt gacacacgct agcccttgtg
    13141 atactttgat ttgttagggg aagccaattc gccaaatatc atttaaattt attctgctgt
    13201 atcgttattg cgtaataagg ccaacaatga acaacccacc tttgacaaaa aatggagtta
    13261 tcatgttgag caactgccta attcagcgtg atattattga ggtaagttga gtaatgaagt
    13321 tatctatctt ttcacgactg gcgttaggat actttgtgat atttgtgatc cttggcatag
    13381 taaatggtta taccctctgg caactgcatc agattagcag gggaacgaag cagatcataa
    13441 atattgatga acgcattttg gaaattaaaa aaaagcttgt tgacaatata ttgtctcaga
    13501 tggaatatga aaagaaattt gccataacca aggattctat tttttatgaa cagttcttgt
    13561 catcccaaag tgactttcaa aattatttaa aagatgctct ttatattgca aatacgaagg
    13621 acaaaacaac atatttgaat caaataaaca atagttattc tctttataat gccctggtta
    13681 tagatgatct tgaatctata aagaacaaaa aacatttttc gatagtaaaa aacgaaattg
    13741 aaaaagaaag actcgtcgac aatatcctcg aagacctgaa agcattggaa acctcaatca
    13801 tgaaagatgt tcaccggcgg atgaacgtgc tcagcgaagc tggtagttcg tcgagaaaac
    13861 ttacgcttat tatgtgggtc accgccttgg ttctagtctt gtttacttct tttttcacta
    13921 ccagaagcat aacaaaacca ttaacatcgt tgatggaaaa gacaaaagag atttcaaagg
    13981 gaattttcaa gagtgatctc aagataacat ctcctcctga aatgatggaa ctaaccagtg
    14041 cctttaatgc aatgtgtgat aagttaaata aggttgaaaa aatgaagtcg gatttctttt
    14101 cgaccatgtc acacgaattg cgaacccccc ttacttctat taaggagggt atagggttac
    14161 ttcaggaagg cgtgggtgga actgtcactg agaaacaaca gaaactacta tcaatcctta
    14221 cagaagagtc aaaccgcctg attggccttg taaattcatt actcgacatt gccaagatgg
    14281 aagcaggcat gatgacatac aattttggcc atgaaaacat aggccttctt atccagcaat
    14341 caatacaaga ggtagcgccc ttagtagaat caaaaaaaat aaaaatctgg ctagaaacca
    14401 cggatgaatt aacacatccc agaatagata gagaacgtat gctccaggta ttgagaaatt
    14461 tgatcagcaa cgcgataaaa ttttcttcag aacacgggca gataacgatt tctgcgtatc
    14521 tagaagagag ggggctttta atttcagttc aagacactgg accaggaatt cccaaggaaa
    14581 acctttatgc aatctttgga aaatttcaac aaccccctgt aaagacttcg gaatggacaa
    14641 agggtactgg tttgggttta gctatcgtga aaaatatcgt tgtcgcacat ggaggtgaag
    14701 tatgggtaga aagcgacata ggacagggaa gtactttttt tgtgctttta cccttctgat
    14761 cactttcgct ttaacatgtt gtaacacaat cgataaaaaa ccgcaggagc ctgaaaaaat
    14821 gagtctatcc attgagccaa ttcatcctgc cgcagttcag gtgccaccca aaaaggagca
    14881 accgcgagat gagtcaaaag aacatctcct tgcaggacag cgattgctct ccatgggcca
    14941 gtatgacgca tctgtcaaag aatacctcag ggttctctcg atagcgccga ataaacctcc
    15001 tggtgatgaa gctagttttg ctattggtct gatatacgct tatccaaaat attcgaagaa
    15061 agatcatagc aaggcagtgt catcactgac aagaatcatc aaggactacc cccatagcaa
    15121 ctggtcgggt catgcaaaaa tccttcttga tttaattcag gacaatgaaa ggttgaggag
    15181 aatctcagca gaggcaattc atgaaaatga aaaattaaaa aatatgatcg aacaatctaa
    15241 aaaggttgat cttgaaatag aggaaaagaa aagagaaaaa gcgaggtaaa ctattttatg
    15301 actttgggga ggattcttgt tgtagacgat gataaaaacc ttgttgattt aatacaaatg
    15361 aggttagaaa cggctggtta tgatgtagca accgccttac acgaagaata tgctattgaa
    15421 aaagtcaagg aacagctttt cgatttagca attgtagatc ttcagcttgt caatactgac
    15481 ggcatttcgc ttatggaaga cctccaccgc attattcctg aaatgcctgt tatcatactg
    15541 acagcgtacg gaagcattga aagtgctgtt gaagctataa agcgtggggc ttacagttat
    15601 gttacaaaac catttgagca tcaggagctt cttctccaga ttcaaaaagc cgttgaaaat
    15661 cgccgattaa cctccgaaat tgaaagattg aaggatctct tgagtgaaag gtacagcttt
    15721 tcgaatgttg taacaagaag cgaaagaatg cagaaggtac tagagacgat ttctcgaatt
    15781 gcaccaacag aatccaccgt tcttttgctc ggtgagagcg gcaccggaaa ggatttgatt
    15841 gcgaaagtga tccatcttgc aagtcaacga aaagataaac cgttcgttgc cattaactgc
    15901 gcggcccttc ctgagacatt actggaaagt aatctctttg gccatgaaaa aggggcattt
    15961 agcggtgcaa tccgcagtac aaaagggctg ttcttacagg ccgatgaggg aacaattttt
    16021 ctcgatgaaa ttggtgacat gcctctgtcc attcaagcaa aagttttgag agtgcttcag
    16081 gataaacagt tctatcctgt ggggagcgaa aaacttgtag aggtcaatgt aagaatcatt
    16141 gttgcaacaa ataaaaacct tgaagaacag gtcgagcagg ggcttttcag agaggatctc
    16201 tattacagaa tccatgttat cccgattcat ttgccacccc tgagagatcg aaaagaagac
    16261 atcccgcccc tcgttgatca tttcctaaac aaatttaggg acaaagttaa aaaagatatt
    16321 aaaggattta cccctcaagc catgcagaaa ctgatgctcc acgactggcc cgggaacgtt
    16381 cgcgagctgg aaaacgtcat tgaatatgca gctgctatga cggataccga gcttatcgat
    16441 gaaaatctta tctttcagac taaagtcata ccttctatca cttatatcaa accgttgaaa
    16501 gaggcaaaag atgtttttga aagaggctat ctaatatatc ttctcgaaac atgtaaaggc
    16561 aaggtaagtg aagctgcaaa attggccgga aaatataggg ctgactttta tcttcttctc
    16621 aaaaagcacg atcttaaccc tgacgatttc aaaacaaagg gtaaacgcag gtctgagcct
    16681 acatagcaat ttttcacctg tatgaagaat ccttcacaaa aaaaatcgtg aacattatct
    16741 gaacctataa tatcttttga tttcagcata atagctcaaa ttgtaccatg gcatacatct
    16801 tgcctttgaa gttatgattg tgtacaacca tataaaattt tggtcattta aggaaataga
    16861 tattgatatg caaaaccgac tgatatataa aaatgtcaaa tccaattacc atagggtcaa
    16921 aaaatcaatg cctctattcg ttttaatctt tttttgtgct atttcattaa ttctgccaag
    16981 cgaaggagac tgtgcccaag caactttcac atgggacaaa aacactgaga cctatgtggc
    17041 cggttataaa atatattatg gcaacagctc agggttatat aactggtcta tcgatgtggg
    17101 taatgtaaca aaatatacca taaccggcct tgctgaaggt tcaacatatt atttcgccgc
    17161 aacagcctat gatacatcaa agaaggaaag caaacactcc agtgaggtga gttttaatag
    17221 ctgcacctat acaatttctc ctactactgc caagtttgat ccgcagggag gaacaggaac
    17281 agtcagaatc tctacgcaga aaagctgcaa ctggaccgcc acaagcggtg taccatggat
    17341 aacaattaat tcaagtaaca aagggacagg tagcggtgaa gtctcctatt ctgtaacaac
    17401 taattcaaaa aatagctcac gatcagcatc gttcaccatt ggccacagca tttttacagt
    17461 caatcaggcc ggtcatacgc aaatcagtaa agatgcaagc agtatcaagg aatatatggt
    17521 caaagtgact ataaaacaaa agaacaaagg tagtggggta gtaaaaagct acgatagaaa
    17581 aatagactgt ggtgatgttt gtttctcgaa ttataagcaa aaatcaactg ttacccttta
    17641 tgctaccccc ggttcaggat caacatttat tggatgggca ccagccactc ttggttgtga
    17701 aggtactgga ccatgtatgg tttcagtgga caaaataaaa aaaatacaag ccatttttgc
    17761 gggagactac gaactaaagt tggttactgt aagcaaaaat ggaggggctg gaagagttac
    17821 aagctcacca tgggccctcg attgcaaatc tggcaacgat ggtaaatgtg atggaaccta
    17881 ccgttacaac agagatgtaa cgttgacttc atatgcatcc ccgggctcta tttttgtggg
    17941 ttggcgccca gaaaaattat gccctggtac tggcaaatgc acaatagcaa tggatcggaa
    18001 aaggattata aaggctgtct tctcgaagcc aataaaagat taactccaaa tattttatgt
    18061 tgttaacctt tcctgcataa agattcgaaa agagacaaat tgatacaaat ctcagaaggt
    18121 ttttttctat ttcttctacc ctgaaaaata gctattacga caccgacatc aagaccttta
    18181 tatggcaaaa catatatgat atatgtatga tatattatca ttgttataac gagaagggtg
    18241 cctatgaccg atatattcag caccaagggg gttgttgatt ccttcatcga acagagaggg
    18301 ttcataccaa ttgtgtttct ccacagaatc gatgccgaga atgaatggaa tatgtagtat
    18361 gatagagagg ttttcttcct ccagcacaaa cgtagtctct ttgagagctt cgttgctgaa
    18421 aatcaacagg cggcatgagg agccgtgtaa cctctagaga atgaaactat atagatggga
    18481 tcttgcataa aacagcacaa agcaaaccca ttaccttaca tccagcatca cataaacaga
    18541 tttgtcatca acatatacta ccgggttgaa agggtattga taccctgaaa caagggtacg
    18601 gcttcacccg gatgcacttc accggatccg agaaagaaaa tatggagttc atgctcacag
    18661 ctatcccctt caacctgaaa agacagcact gatgatggga acatagggga tatattccca
    18721 aggaagcaag aagatgaaaa aggaggtaaa aatatatcaa aaaatgatat tatgccaaaa
    18781 aacttgtacg aaataaattt aatcctcatt aaatgcctca taaggggtga aaataccatg
    18841 catccccctt atggcaggaa tcagaggtta tgtaaaaatg ttaaaaagat gaagcgaaac
    18901 agtatctagc ccaatcgttt cgtcaaaatt tctttaagga gtttggggtt tgccttccct
    18961 tgagatgcct tcatggccag acccataaaa aaacccataa tcttggcatt tccctgacga
    19021 tattcctcca catccgaagg atgatctgca aggattttgt cgattaaccc ttcaagttca
    19081 gcagtatctg aaacctgtcg taaacttcct ttctccacaa tctcttcagg tgattgatcg
    19141 tccgacagta gttgaagcag cacctctttg gctgcgtggg catttataac gcccgactcc
    19201 agcatagtca ggagctcggt gaatctattt gtggtgatgg tagtctccct gatggtctgg
    19261 ttcctgtccc ggagagcggg catgaggtgg gttactatcc agcttgccac tcttcgcgct
    19321 ggtaatccct ttctcaccac ttcctcgaaa aactctgaca tgtcccgttc ctggctcata
    19381 cctgcagctt cttcaggggt gaggccgtag tccttcaaaa aacggttcgc cttctgcacc
    19441 ggcatctcag gcagtctcat ttgaatctta tccagccagt caggggtaac cacaattctc
    19501 ggcacagacg gatcaggaac acagggcccc gaaaattttt cccgcattgg taacgttacc
    19561 ctcttatccg gatcccagag cctcgtatgg agcacaatgg gttcgccttt ctcaatactc
    19621 tctgtttggc gcttgatttc atatgctata gcttcggcca cattgcgaat agagttcatg
    19681 ttttttactt ctacttttgt gttaaaggca tcgcttccta ctggccgcat agagatattt
    19741 gcatccaccc ggagcgttcc ctgttccata ctgcactcag cgctaccagt gtaacgcaac
    19801 tgggtcctca atgccttcac atattcaata gcatcatggg gacttcgtaa atctggttca
    19861 cttacaatct caataagtgg cgcacctgca cggttgaagt caaccaggct gatgggtgtc
    19921 ttcccttctg tttcgtgtac gagtctggca acatcctcct ccatatggac atgatgtatt
    19981 cgcagtcttt tcaattcacc atcctcacca ataatgtcaa tccacccgtt tctcgccagg
    20041 ggcttatggt gctgggagag ctgatatccc ttcggaagat cgggataata ataaaccttc
    20101 tgatcaaaag cactctctgg ctgaattgta cagttaagag caagacaagc gagggttgcc
    20161 ttctctaatg catctgcact cagtctgggc aaggctccgg gcaaccagag acaggtggga
    20221 cacgtgttct tgttcggttc atcacccgga ctgttggggc agtcacagaa aagtttcgtt
    20281 atagtattca gctgaacatg tatctcaaga ccaattacag cttcgtacct catcagtctt
    20341 acactccttt gactattgat gataactcca tgcctaatgc cagcaaacgg acatcgcttt
    20401 tgtgctcacc aagaatctgg agtcctacat cacccgtgga atcgctagcg aaacgaggaa
    20461 tacttaggga tggctgtcct gttacatttg caggtacggt aaaagaaaat gcctcataag
    20521 tttcagctat ggtagtcgca ttcatagcat caaacagtag cctccgcgtt ggaagagcaa
    20581 tcacatctac agtctccagg agcctcgaca ttttccggac tagcccgcct ctgattctgc
    20641 aggcatcttc aaaagaagga tagttctcaa actggaaata tgccccctga aaaagatatg
    20701 atttgattag ggttccgaat gattcaccac gagttttaag atacatatca ttccagttct
    20761 tgtcagaggc tgctcggtgg ccataacgga ctccatcgta tttcccagaa ctcgaagaag
    20821 cttctacaga accgatcaca ttgtgaacag cacgaaagag ctcaaagttt tcaaaactca
    20881 actctttaat cttaaaccct ttatctttaa gtttcgtgat tcctgccttg aatgcgtttt
    20941 tttcactttc attcagcatt ctatggcatt cctcaatgac accaactgtg ctgggtatcc
    21001 tcaacgattt cattgactca ccaagatcag gtaacggctc atcagccatg gagaaatcgt
    21061 tttcatcaaa acctgcaatt acctcagcaa cagcaataac attttccagg tgccttccta
    21121 cgataccaca gctctccatg gatggtatga gtccaatcat gccaagacgg gatataattc
    21181 cgaagctcgg tttatatcca aacattcctg atacagcgga tgcaatacgc gcttcaccca
    21241 tggtgtcagt tatcaagcca attgtcacct catcaccgga acaagcctcg gccaaggtat
    21301 ctccgcctag gccgaaccca agttcactca ttcgtgcact gcccaccagg gttgcccccc
    21361 cgctcctgag ccgtttcacc accgtagcgt cttccagggc aacgtacccg ttgagggctc
    21421 ggctgcccgc gtttgtgggc catccagtga cagacatatt gggctgaact acaattttca
    21481 aacctgtcaa aggcccctcc cgagggcttg aaatatgtga tgtctcttta tgtgtataga
    21541 tatgacacgt catcagactt tccttattct aaaatgcttg caaccttgaa atatctgccc
    21601 ttcatagcag gtgcattttt aaagagatca cctcgttcag acaactggct gattcgggct
    21661 tccttccctt tacgggttcg gttcataggg gccacaacag aaaataacgg ctctccatcc
    21721 tctttccgct cacttacaat agctgcaagg ttttcggcgt ccttcaccga agccattatg
    21781 actgcccgtt catcattatt gattttaata cgagaaaccc aggagagcga atcaatcaaa
    21841 tcttgttgtt tctctgcacc aggcaatgac tggtctttgc ctagatcgag aaggccgagt
    21901 tctgcgagct ggacactcga aacctgagaa ggggcctgcg tcagagggca ataggcgctt
    21961 cggttctttg gaaaagctat gacctctctg attgaagttg tgcccagaat catggcaacc
    22021 actcggtcca cgcccagtgc aattcccccg tgaggaggag caccgtactc gagggcgcgg
    22081 agaaaaaaac caaatttctc ctccacatcg acatgggaaa gtccaagagc tgaaaatatt
    22141 ttgttttgca agtctctatc gttgatgcgg atactacctc caccgagttc ctctccgttt
    22201 acaacaaggt cgtatgcacg ggagcgaagg ctcaaaagtt cttctatgtt tttaggatca
    22261 aaatcagtcc ggtcaggcat ggtaaagggg tggtgactcg aagtgacacc atcatccgtt
    22321 gcctcaaaaa gaggaaaatc agtcacccac aggggacaaa acattccact cggaatgagg
    22381 cccaatcgat ctgcaatgtg caaacggagc tgacctagag ccgaacagac aagataccag
    22441 gaaggatcag caatcatgag gatcacatct cctttctgcg cggtgaagcg tgtgaggatc
    22501 tcactctgct cactttcact aaaaaactga actatgtttg actccaggcc accatccaag
    22561 accctcatcc atgtcatacc ttttgcgcca aagccaggta caatctcttt tgcatattca
    22621 ttctgaagca cgttcttact cagcttttcc gattggcctt tgacattaat ccctttaata
    22681 catcccccgc gttccaagat ctgtttgaaa ataccgtatt ttgtatcact aaaaatatca
    22741 gtgcaatcac gaaatgtcat atcgaatcgc aagtctggcc ggtcagagcc tgttgcattc
    22801 attgcatcga gccaagtcat acggggataa gggcgaggga gtttaatccc gccgacttcg
    22861 aacatacgtg ctgacaattc ttcgaacagg gcatagataa attcttcatc gataaaagat
    22921 gcctccagat cgagctgggt aaattccggt tgccggtttg gacgtaaatc ttcatcccgg
    22981 aaacatcttg caatctgaaa ataacgttcc attcccccaa ccatgaggag ttgtttgaaa
    23041 agttggggcg actgtggaag ggcatagaaa tttttcggat gatgcctgct cgggacaagg
    23101 tagtctcgtg caccttcagg ggtgctttta gtcagtatag gagtttctat ctctacaaac
    23161 ccttgttcat cgagataatc tcttacagtt ttcacaatgc ggtgcctttt gatcagatgg
    23221 tcctgaattg tcgggcgacg gagatcaaga taccggaatt gaagcctcag gtcctctgcc
    23281 acaggttcat cccgtttcaa agccattccg gcaaccatgg acttttctga aatcggaaat
    23341 ggcaacacac cggatttgct caaaacagtc aggctatcag cctttacctc aacatttccg
    23401 gttgcaatat tagggttctc tgtaccctcc tgccttttaa ccactcggcc gcttattaga
    23461 acacaaaatt catttcttaa gagtcctgcc ttttcgcaga ctgtttgatc cgtagactca
    23521 ggtccaaata caacctgaac aattccagag cggtcccgta gatggacgaa aagcacctct
    23581 ccatgatccc taagtgcatc tacccaacct gctagtgtca caacccttcc atcatcagat
    23641 atattcagct gcccacagtg aatccgatca cggtatgaca tgtgcgcccc atcctttctc
    23701 atcttagcca attgcttatc ctctgatgtg tgtgagttga tgagtaaaag ttaccattga
    23761 gaaaacacac ggtcaataga ataatggaag aaaagattag agtagaattt aaaataacat
    23821 ggggctaaga gtaaagcata tgggcgcaat ggacgatgga agagaaaaat gtgataagat
    23881 aattagagtt atgaggaaaa agaaaaatca tcaggatctc acgagagaca ttgttctcca
    23941 tgtttttaaa caggtgaaga gacccatagc acaaaacgaa ctggttgcta tcctgtctct
    24001 aaacaaaaaa caaggtaaag ccttgaaaca tatcctgaga gaactcgttc gagatggatc
    24061 tgttttgcag cttaaaaata aaagatatgg tctgccgcag gaaatgaacc tcattgcagg
    24121 caccctctgg ggtacacgaa gtggaagcgg atttgtggta cctgacaacg tgaatgaaaa
    24181 agacttattc attcctgcca gatttatcaa gaacgccttt cacggggaca aggtaataac
    24241 cagagtagaa cgtaacctca gaggcagaag agaaggaacc ataattaaga tcatagggag
    24301 aaaaataaag aacattgtcg gattcgttcg acatcacagg gacttaatgt acatcatccc
    24361 agaggatgaa cggatatctc atcattttat tgttactaag ccaacaaaat attcaggcct
    24421 caaggatgac catcttgtgg ctgccaggat aacaagattc cctgatggag ggatcgatcc
    24481 tacatgcacg attctcaaag cctttgcagg cctcaacgat gtccactcga taatccagtt
    24541 cattcagtac aaaaactccc tgcccttccg ctttaaaaaa agaacagaaa atgaagcaca
    24601 agcacttgac ctatcaattg acagacagga cagacttgat ctccgtgaaa ctatacatgt
    24661 tacaattgac ggtgagcttg caaaagattt tgacgacgca gtctgcgtag aaaagaatga
    24721 tcatggtttc actctttatg tctcaatagc cgatgtttct cactatgttt tgccgggaac
    24781 tgatcttgat cgggaggctt atgaacgggg aacgagcgtt tattttcctg gttcggtaat
    24841 ccccatgctg cccaaaattc tttcaaacga catttgcagc ttaaacccga atatggatag
    24901 gtttgccata acggcaaagc ttattttcaa tagaaatggt gatattacca gcagttcatt
    24961 tcacaaatct attatcagaa gctttcttag gctgacctat aatgaagtgg aggatgccct
    25021 cgttaggaag gatcgggcag tcagaaaaga gattcagaaa atccttcctg ctttggaata
    25081 tatgggtgag cttgctgcgc tcctgcacac aaaaagggaa aggttaggca gtctcgattt
    25141 tgatctacca gagctgaata tcatacttga cattaaaggc gggcttcgca acatcctgaa
    25201 gactgaacgc cttttttccc atcgtatcat cgaggaattc atgattgtga caaacgaggc
    25261 agttgccaaa ttcctgacag aaaataaggt taacactatc tacaggatac acgagcctcc
    25321 agacgctgaa aagctcaggg attttgagag gctcttgcag acgttgtcaa ttaaatataa
    25381 gcgggataac aagaacatta gggatcttca aaggaccctg atgagtttgg agggaacaga
    25441 ctacgagttt cttgtgagca ggatactctt gcggtccatg aagcaggcaa ggtattcatc
    25501 tcagaataag ggccactttg gcctggcatc ccgctgttat ttgcacttta cctctcctat
    25561 caggcgatac cctgatattg tctgtcacag agccctcaaa aacattctgt ccggcaacgg
    25621 aaaaagatat ggcgagacag agctcgagac catggccaac tatctttcca acagggaaag
    25681 aacagcaatg gaggcggaaa gagaaattgg agacagaatg cgagtccttt ttatgaagga
    25741 caaaatcggt caggaatacg atggtgtaat ctcacgtgtt gtatccttcg gcttttttgt
    25801 ggaacttgca gatgtttttg ttgaagggct tgtcctggtt aatactcttt atgacgatta
    25861 ttaccattac gaagaggaaa ggttccgcct gatcggtcgc agaatgcgga atatctatcg
    25921 tttaggagac agggtaaaaa tcaggatcac tctggctaac gtagaaaaaa atcagctaca
    25981 cttctcactt gtaaaaaaaa tatcagattc caatagatag ccctgactag ctacatcagc
    26041 cggccttaaa gcgtagagct ttggcctata caaaaaacca agcaagcttt tttaccttac
    26101 agcggtttgt cctcggaata cctgacagca gctctcatat cttatttgcg taaaccctat
    26161 gatagcaaaa ctgtcagttc agtatgctga agctcgcatg aatcctctgt ttacacactg
    26221 gacacagagg ttcaatgagg gtgaaagtct taccgaagac atcaatctcc ctaaaatata
    26281 ccgctggtgc ttcggtaatg tttttctcat cgtagtattt accgcattca cacactggta
    26341 gagcttggcg tactgtctga ccgtgaaaaa cctgatccat atgggctgtc accaattttt
    26401 tttcttccct tgtcatgatt taaatatata tctgcaaagc aacatataca accccaaagg
    26461 gctctcgtat tgaatattat tcataacaat gataatctaa ataacggctt ctatcattat
    26521 gaggatccct caacctatac cccatgtttc gattttggcc tggcacgaaa gaacaaaaca
    26581 agttgacaca cctttggttt ttcgataact tatatgagat cttttttgta ttctacggtg
    26641 cggtatccat tgccagccgt gatcaaacaa agtatcgtta gctctttctc aaaaacaaaa
    26701 ggttataaaa aggttcaatt ataataaatc aggaggttgt catggcatca atccacggta
    26761 taccgaaatc cccctggagg gcaatcatag agagcgcttt ttttgccagt tctgtcagaa
    26821 agacatcgat ggcagaattg tatgccctcg caaaaaaaca accggaggtg atggtaacat
    26881 ccgaacccat gtatagacct gaacaattcg accttccaca cgatgcaaag gtccttgttt
    26941 ctaatgatgg aggaattttc ggccgtactg cccgggcaag aagacttgta agaaccttca
    27001 ccaaagcaga taaagacaaa taccagagca tgctgtcaga agctacctat catttgaaca
    27061 ggcaacctgg attgtggctt gaaggcatga taggtctgca ccctgatttt atgatcaaag
    27121 cacaccttct aagccctgag acagatgcca agaacatgct tgactggggt attaacttca
    27181 ttccttttat gcctccctgg tcagacctct ataaggaatc aaggcagctg ggagagccag
    27241 atatccttgt actggcatac ccagaatggt ccaaccctga atttaaagac ggcctcgtta
    27301 tcattgacga agatcagaac tgtatagcca tccttggcct taggtacttt ggagagagga
    27361 aaaaggggac gttgacaata gcctggactg tcggcgtgag acataacatg gtggcctgtc
    27421 acggcggaat caagaaaatt ggcgacaaac cacctgtagc tgttttcggt ctttcaggtt
    27481 caggaaaatc ttctattacc aacagtagcg atcatgaagg tactctcaca ccagaagaga
    27541 aagtgactgt cattcatgat gatgctttcc ttattgacct cgaccataac ctcagcatag
    27601 tcctggagcc ctccctcttt gataaaactg acgccatgga atttgacgat cccgttgtaa
    27661 agtattttta ttcggcacaa aacatggcag ttacagggat ggcagatggc acacggaggc
    27721 ttgtggggaa ggacataaga aacgataacg gacgctgcct gaaatcaaga gatatgttca
    27781 atcacgctaa cttttgcgag aaaccttcaa cagtaatatg gcttcagaaa gacacaagcc
    27841 ttcctccgat ctgcaaggta acccgatctg caatggctgt agcaatgggg gcctcgttga
    27901 gcactctgcg tgcaaaaggc gtagagaatg ccgatccacg agattttgaa agacttgtta
    27961 tcgaaccatt tgccaatcct ttcagggtcc acccccttgt ggaagactgc cgccaatttc
    28021 tcaaactttt caaatctggc tgtgaatgct atgtaatgaa cacccatgcc gtaggaatgc
    28081 caggccacct gactgatatc ccgaagacaa tctccctttc gatcgttaca gctcttgtga
    28141 gaggaaaaat agagtggaaa gattggcgag ccttcagtgg tctgcagatt cccaaaaacg
    28201 gtaatgagct ctttggccct gattatgaga agaaatatag tccgcctacg agacaccctg
    28261 gctacctcag gtttctaaga gaccggatgc aggacaggat taccttcctc tccaacaaaa
    28321 gagaccttga aaacgacatg gagagcatct tcatcgatcc attggtagca tcccggacag
    28381 tcctcgacca cattctggag ccactgtgag tttagaatct tgtgcagaca aggcctaatt
    28441 taaaccttgt atacggtcat tgatgagaaa cctgacaagt ctgcatgtca ataggccgag
    28501 cttccactgt ttctttcggg ataaaacatg agaatcttct tgatgcatct ccggcagggg
    28561 tataagtaga cagcagttca tctttcttct ttcaattcta tagcttcgac ctttaccctt
    28621 gccagacctt tcttatggaa gccaagtctc ttggctgcac cctcactcac atcgataatc
    28681 ctgtcaccag acgaaggatt gtagtcgctg gggaaaggac ctctgtcgtt gactctgaca
    28741 atgacccact gatcgttctc caggttggtc accataacat gggacgggag ggggaggtac
    28801 ttgtgagcgg ctgtaaactg tctgggatca aaaacttcac cgtttgcagt cattcgccct
    28861 ccttttgtcc tgagtgtctc gtaaccatac cacgatgcaa ctccaatctc ttcataggtt
    28921 tttgcttttt caatgctcat ggggtaatag gtcttacctt tcaccacata tggtgaattt
    28981 cttacacgtc cctgttttaa cgcttctttg ggcggcaatc cgtctatagt ttctatatcc
    29041 tcattgacaa aagcccattc cataggtgcc ttaactgctt tgtatgtgaa tttaccaata
    29101 tgataaacaa cttttgttgt tccagcggtt atctcgtatg tgccttttac aacataatag
    29161 gttcctttca cggtcccttt gataaccttg tatggggcag ttatcagcca gcatcccgtg
    29221 agagcaaggc acaaaatcaa ggaaatactt acagggttaa agagaggttt cactttgaca
    29281 aaaacttgtt gtaaagcttt tttactgact ccaacaggct tttccggaac tgaacccgga
    29341 ggtaaagatc ggcctgtgac ctgtctccat aaaaacctgc agcaccttta agacggattg
    29401 tctgtccttc cctcacccca acaggaaccg taacaacgag ctgccgtgat gttgtcgggt
    29461 caatataaga gaccttgcca ccacggctgg cctgctcccg actgattgtg attataccat
    29521 aacgatccgc ggtttgctga ctcgtatacc gtcccttaaa agcctttctc accatataac
    29581 cagcaagttt agccagcaag ccagaaacaa aggccgtttt tgaagaagaa aaagtgttct
    29641 gttgattccc gtgcctccca aaaggaaacc ccgtaaagat aaaacctttc ccgaaaatcc
    29701 ctgaatgttt gaattcgaat gtcttgtatc caggtccata aaattcacgg aagagatcgt
    29761 tgaaattcct gaatccgaac tgtcgtgtca tctcttcgaa tatctggttt atatccgaac
    29821 cacggaagat atcttgctca gaatacccct tcctgaaatg atcatacccg tcatacccgt
    29881 attgttggtt catgctgtca taccggtttc ttttttcagg atcagaaaga acagcatatg
    29941 cttcgtttaa ttctttcatc ttctcaacag cgttcaaatc tcctttattt ctgtcagggt
    30001 gaaattcgaa ggcaagtctg cggtaagtct ctttgacctg ttggtgtgta gcgtcttttt
    30061 caataccgag tatcctataa taatcttttt gcctcacatg gtctccttat ttccagaacc
    30121 ttccggtaaa cagtacaaca atcgtaaaaa tttctagcct gcccgcgagc atcaaaaacg
    30181 ataatatcca tttagccaac acaggaacat cggcgaagtt atcaaccggc cctaccgttc
    30241 cgagtcctgg cccaacatta cccaaggttg ttgccacagc tcctgctgca cttaatatat
    30301 ccatacccaa agatgacact acaattgtac caaaaatgaa gaaaagtatg taaagcatca
    30361 cgaacgccat aatgttagat acaagatttt ctggaaccag ctttccttca agttttattg
    30421 gaaaaacgcc acggggataa aaaagttttt ttacctcacc cccaatatat ttaaacaaca
    30481 caagaattcg aacactcttt atggcaccac ctgtggagct ggcacatccg cctgcaaaca
    30541 taagaagaaa aagcacaatt tgcgttgctg ccggccataa ttcataatcc tttgttataa
    30601 atccggtggt ggtaattaat gatactgtct gaaaaagcga atctcttact gtttcctctg
    30661 agagctctgt cccagcaaaa aacctaatca atacgataaa aattgttgag cacattatga
    30721 tggacaaaaa caaccgtaat tccgggtttt tgaaatagtg aactggtttt cctcttaatg
    30781 cccagtaatg aaggttgaaa tttataccgg ctaatgtcat aaaaagaatc gtaatgtaat
    30841 gtacgtacgg tgaggaaaaa tggccgatgc ttgcgttaag tggtgcaaaa cctccagttg
    30901 ccatagtgcc gaaggttata catagggaat cgaaaaagtt tgcgcctgcc agcataagca
    30961 tgattgtttg agctattgta aagatgaggt atacacccca gagcagtttt gctgtatctc
    31021 tgatgcgcga tgatacctta tcttttgaag gaccggggac ctctgcctta aaaagatgag
    31081 acccacccat cccagtgaat ggaaagattg ccactgccag agcaattaca cccataccac
    31141 caagccattg ggtcatattt ctccatagca ataagctttt tggcagtatt tcgatgtcgc
    31201 ggagaattgt cgcgcccgtt gtggtaaatc cggacataga ctcaaaaaaa gcatcggtca
    31261 ggctttcaat agatcctgtg gttaagaaag gaagacatcc gaaaaatgtg aagctgatcc
    31321 agcaaaggtt aacaagaaga aaaccctctt ttgttgttaa ttctttaggt ttggagccaa
    31381 agggccacac taaaccgctc aagatcagga taccgattgc aatcagtata aaatgataat
    31441 ggatgccatc tttatagtag accgaaaaac agagagggag aatgaaaaac atgcctgcaa
    31501 taatccagag tctggagagg acgtaaacta tgttttttct tttcaaaagg aggttacctc
    31561 gtcaagaaag cctactctcg atagctttta ttgcatgcgg caaagcaaaa accacaattt
    31621 tatcgcctgg attaatgatg gtatcaccat agggtacaaa aacctctgtg ccccgaacaa
    31681 ttgcggcgac gagtgcatca tccggcaatt tcaatttttt aagcggtttc cctgtgagcc
    31741 tatcgtttgc tttgacttcc acttcaatgg cctctgcatc gatgccatga aaagtggcaa
    31801 cagagacaat cccccctctg cgaataagcc tgaatattgc gtttaccgta gatatctgtg
    31861 tatttaccgt cgaatcaatt ccgatgaccg gcatcagtgg cagataattt accttattga
    31921 ccagggctat tgttttacgt atcccaaggt gtttggcaag caaacaggct atcatattat
    31981 tttcatcatc agaggttact gccacaaggg catccatgcc aagaataccc tcacgaacaa
    32041 tactatctat ctcggctcca tcagcgtgca tgaccatcgt tttctcaagt tgtcctgcaa
    32101 tctttagaga tttcgcttta tccgattcaa cgataatcac atctattcca cgtttttcaa
    32161 gggactgggc aacctttctg cctatttttc ctccacccag aatcataatt ttttccagtt
    32221 tttcatcggc tttcccagct atcttcagaa gctctggcag atagtccttt ccagtcacca
    32281 caaagacctg gtcgttttta ttgatgtaat cctccccggt tgggatgatt gtcttgtctc
    32341 ctttgaggac tgcaagtata cgaaaatgcc ttcgttgttc tgcggtaccc atatctttta
    32401 attgcttgtt aagataagga aacccaggat ccaccttcat gcccagcata agaatcttgc
    32461 caccctcgaa ttctataatt tctgaggcag tggatctcat gagaagcctt acaatctctt
    32521 ctgatacctc ctcttccggg tgaatgaaca agtcaatccc aagatcatcg gctttaagta
    32581 tggagtcaga cctgtaatac gctgatgtcc tcactctggc aatctttcgc ctgactccaa
    32641 gtttagaggc aaaaagacat gccagaatgt tcacttcgtc cacgccggcc gctgcaatca
    32701 gcatatcagc ggaaccgagg tttgctgccc tcagcacctt ctggtctgtt ccgctacctt
    32761 ggataacaga aacatcaagg atttcactgg ctctgttgca tttttcaggg ttttcttcaa
    32821 taagaataag atcatggcct tcatgggtaa gacgctttgc aagattaaat cctacatccc
    32881 ctgcaccaat aataactatt ttcatgggtt ttccgatata aatccagttt tgattatttt
    32941 tagcagaaac aggaacaccc tgtcaaacag gtattcctat ccacgtgatt agtgcataaa
    33001 ccggtcgcaa caaattcaaa cctaaccatc tgggatgtta atccatataa taacttcagt
    33061 aaaccaaata aacaaataca aggtgtacgc tgttcaaagt ggaatttaac ttctgtttac
    33121 ccgttatatc atgtattttc atcatgttat gtgggcattc ttgaaaaggt tgatgtcaat
    33181 gtcctggata tcatgaatct ccaccagaaa taacctgagt agcgataatc ttcctcatgc
    33241 tcacaggcgg atgtttcctt tcctgaccgt gaagtataga ttggacaaaa tctataatat
    33301 gcatatggtt catcgagacg ggtagagcct ttattttata aaaggtcact gacctgcgca
    33361 gtcctgccta cttaattgtg caggttcttt atagaaggac aaattctgat gcaccccacc
    33421 ttttatttta ttgttctaca gcttattcct aattcgacaa tatgtctttt ctccaccgtt
    33481 tgacaaataa tattacaaac ggattcgtct tttttcaaga tagacacaag atgatagagc
    33541 gaagatcatc agcagccaag gttaggacat tcccggtaac gtaaacctgg cagcattgaa
    33601 ccttattacg gagaaactct tcttggaata tcttctctgt gccatccata ataactaatt
    33661 ggaacatatc tcgatggctt tccatcatgc cagtgctgat tccctatccc agctctgaat
    33721 gagaagaata aggcggggta ttatcttctc ctgataacca tagggttaag gaagcgattt
    33781 caacgttgct gcgaatgtac atgcagaaat tgatgccatt gatgatcaga tattagatat
    33841 tctcagggga aatgttgcag atacgccaga ctttgatagc atatttcagt tcggtatttg
    33901 cagtactcac ttccattgcc attgcaatct tcagatatct tcatcacaac atctcatacc
    33961 tctttcatac tcaaaagatc tgaatgtagc tgccgttttg ataatcggtc tggctatgtt
    34021 tacgctcata tttatctctg cttggataaa tatcttagtt cttggtccat gaggttggta
    34081 agaaaagcgc cgaccgctcc cttgctgctt gcactgatat ttttgtgagc aatgcctcaa
    34141 cctccttgat tagcaccaat gctcctgtta tattcacctt ttattccgtg gatgttcctc
    34201 ctccatgaaa cagctttgtg cttcgtcatc acctaatttt ataggaggtt catccttttt
    34261 taccatccac ttcgatttac ataagagggt tactctgccg atatccttgc aggaatgcaa
    34321 aagtgtattg gtaccgcgag agcggaaggg gttcaggggg aattatttta attggacatc
    34381 agtgaaattc gaacctcttt tttccagtca actttcctga ataaaaactc aaaaagaagc
    34441 aaaataatgc aaattagaca aagtttgcaa tttattgtct ctcctgcttc gggaagctgc
    34501 tattgcaaca ccaacatagg cattccgtcc ttactcccaa ttcaatcatc ttgcagtctt
    34561 atatttccac tccggttttc caatctgatt caataacggg aaagagcgat gtttttaaat
    34621 tttaagaaaa gtctgagagc acacagcgca ccacagcagt tgtcagtgtt ttcttcagta
    34681 taatgcctat ctttttttgt tgatcctgtt aaaccatttt tcaaatcatt ttctctcgtc
    34741 aagtttttgt aactcttcca taagagacac catgtcatcc atcttttctg tacggaaagc
    34801 cctatcccgt agcacccttg ttttcggtat accttttgta taccaaataa aaaatttgtg
    34861 aaaaatactc aatgctcttt gctccccgta atgacgtaca agaagattga gatggtgatt
    34921 cataacatcc acacgaatgc taacactggg catatcacct atagaaccat ccctgaaaaa
    34981 ttgtatcaaa ttactgaaaa tccagggatt tccaaaagag ccacgagcaa tagcaacccc
    35041 atcacagcca gtaatatcaa acattctctt aacttgggaa acatttacat tatctccgct
    35101 ggcaatgacc ggaattttca gagcatcttt aacttttttg attgtatcat aatctacagt
    35161 cccactatat ccctgcatct ttgtcctacc gtggataaag agggcactga ttccggcatc
    35221 ttctgcacaa agagccacat ctcgtgcatt tactgaatca atgtcccatc ctgatcgtat
    35281 ctttacagtc acaggggtct ttgtgtgtcg aaccaacgtt tcgagaattt tctttagctt
    35341 ccgtggttct ttgaggagtg atgccccctt tccttttcga acaaccttag gcgttggaca
    35401 cgccgcattc aggtcaagaa ggtcatactc aatatggtca agagacttca atgctctatc
    35461 tatatctttg ctatcaattg caaggagctg aactccaagc ggccggtcct cggcatttga
    35521 agcaagcatg cgttttgtcc ttttatcaaa aacgctcaca gccttcacat cgatcatctc
    35581 tgtgaaagca aaaggagcac caaaggatct gttaatcatg cgaaaaggta gatcactcac
    35641 accggagata ggagctaaca cgcaggggat agaaaaggat aacccgccaa ttgagagcat
    35701 tttctatgga gcgccaattt aataaaaaac tttatatggc taacataaat tgtgttttaa
    35761 tacaatgata tttaagtatt gaatattact tgttggatga gaatgtaacc gtagtttgct
    35821 ggttttttat gaaatctctc acataatcgg gaaaaacatc catttttttg attaatgacg
    35881 gcataatgtg ctggctcata aggctttcaa ttgagtggtt gtgtaagttg acaatgttta
    35941 gatattcctg tagatatgtg ttcgagaatt cagctttttc attaccgctt aaataatcaa
    36001 aacaaagatg ggctatatag agaatagcca cattttcaat acagttttct ggaatatatt
    36061 ccggggagca gaaatttgga tatttttgat attcaacgct ttgaccgata attaccgggg
    36121 tattccaagc atcaagaaga agtgagccaa ctttacaatc atccagtcgg gccatgacga
    36181 tgtccagttc tggttttttc gcttttgaaa caagaataac acactttcct aaatcatgca
    36241 ggatcccgag ggtactcatc atgacaggct tacttttttt ggataattgg gcaatatcaa
    36301 aagctatttg cgatataacg gcgctatgta gatttagtgc cttgaggtta aaatgcctag
    36361 gcattacccc ctggagaaaa ttttccatta gtatctggta tacttgatta aatccaagaa
    36421 gaacgaaggc atgatgaaaa tcagatatct tcctctgaaa gctatagtaa ccagagttga
    36481 caattttcaa gacgatactc acaatcgacg gatccatctt tgcaaaacca gctatttcag
    36541 caacagaagc accttcatcc aagagcattg aggttagtct gttgatctgt attgggaggc
    36601 gcgggaagtt ttttatcatg tcttgaatca tgatcgatcg ggcatattga tcattatcat
    36661 tataaaaatc ttttacatag gatactaatg attctatctt ttttgataat gcacctgatt
    36721 tggtcacaag ctcatcattc aactctgtaa atgtatccaa tatctttcga taaacagaga
    36781 tctgaatacc gggtggaagt aaattaaagg caaattgatc tatttccagt attcttgagg
    36841 ctcctgatga taccacggaa aaacgatttt ttttatcttt tgacaggtca ggatctccaa
    36901 acaaactccc tttctttata ctaactgaaa atgactcgcc atcgttttca ctaaacagta
    36961 tttttgaaga tccatccaga ataaaagtta atatattgca tgtttcatcc tttctgagga
    37021 catactcacc attttcaacg ctccgtattt taccggcctg aaaaagagcg atcgtctctt
    37081 tctctgtaaa cccttcgatg gtggtcgaag atgagaaggt aggagaagat tttttattca
    37141 attcctgtac catattttat cttgttcgat aaggattact tcattattcc tttgtatttc
    37201 cggcaaatac tataaatcct ttatcgccag acagaccacg ggcttaataa ttatcttgca
    37261 aatttgactc agatatttta ctcatagaaa tatgcttcca aatctgggtg tttttgatat
    37321 tttttacaga ggcttgtttc atcccttcta ccgaccaaga tgtctgagat cacccttcaa
    37381 gctcgctttg gtggcaaaaa ttttcattga aaacgggggc cacacagaga atcatgtttt
    37441 tgtgcttgga taaatttggt tattttgact cataaccatc aacagactga catgtaggat
    37501 tttttagcac tatatattga caataactat ttgatttgac atattcaaaa tcactttaat
    37561 ggaggattta tggatgttgt aacagaatta tttgatacag gtgtttacac aattacactg
    37621 aacagacctg ataagaagaa tgcaatggat tcagcattac ttcaggggct caatgacgca
    37681 ctcaaaagag ctgatacaaa atccccgcag attattgtca tcaggggttc aggtagggct
    37741 ttttgttcag gaggagatct tatagaattt aatgaaagcc ctgatcctgg aaagcagatt
    37801 gacggcatgg cagaatgtct ccatcaatca atcatgctta taagaaatat cggagcaatt
    37861 gtaatagcag ttgttgaagg tgtggcagtc ggtgcaggcc ttggactttc acttgcctgt
    37921 gatctgaccc ttgcatcaaa aaataccatt atgaatcttg ggtacagacg gattggtctt
    37981 acacctgacg gcgggggcag tatatccttg ccaaggctca ttggggcaaa gagattcaac
    38041 gaactctacc tcttttcagc aaacgtaaat atgtctgaag cattgatgtt gggtcttgtc
    38101 aattttgtat gtgatgaaaa tgagatagaa gtgaagctgg ctgaaattgt aaaaaatctg
    38161 ctcagtcttc ctatggaaac aatctcacgg ttcaagactc ttgttaataa ttctatgttt
    38221 tcagggcttg aaggtcatct ggatatggaa cgtcagtatg taaaagagct tggcgcacag
    38281 cctcttttca aggaacggat agcgtcattc tttaagagaa agtagaccct atgatctgtt
    38341 aaaaaagaag gacagaaaag gcaggaatgg tcggtaaaag tatgacaggg ttcgagatcc
    38401 gattctccaa acatgcttga aaacttacaa gttggcaggt ttgaagctta accaacaacg
    38461 tgaggctttg taattttatg gctcttattt atcttttcac cattgttggt tacataactc
    38521 tgctgtgggt aacaggtgct tttgttacaa gaaagtccgg atcctcagaa gcctatcttg
    38581 tagcgtcacg gggtctgtct gtaactttta tttccgttct tattgcagga acctggatag
    38641 gaggagtttc catagttggc atggcccagg gagcgtacat atatggtatc agcgctctct
    38701 ggtttcaggc aggtatctgg atcgcaatgt gtgttaccgc ccttttattg aataagatca
    38761 tgaagggaaa taaaacctac tccatacttg acgtggtcgg gaacctttac gatacaagga
    38821 ctgcaaaatt tgcagggata cttcaactta tcttttccat atgggtggtt accatgcagg
    38881 ttgtaggtgg gggggccatc cttgctgtca ttttgaatgg acagatatct tttcagagtg
    38941 gcatgcttct gaccgctatc gttttcaccc tgtataacgt aatgggtggt tttgttgcga
    39001 cagcatacac aaaccttatc catatcggag caatcgtgtt tggaatattc ctcggcggtt
    39061 tttatgtcat gcttaatgca gggaccatca gccccatggc acatcagtca cattatttca
    39121 caccttttgg tgaccttggt ttttttcagg ctgtgagctg ggcgtttata aattttaccc
    39181 ttggagtgct ggctcagccg gttattaaca cggcatcctc tgcaagaaca tcaaaggaag
    39241 gaaaacaagg tattctgata ggaaatgcta tcgcaatccc ggtggttgta atggctgcat
    39301 tgtgtggcat tgttgcaaaa aatgtttttc cccatatccc atctatctca gccttaccag
    39361 cgcttctcga tatcgttcca ccctatgtcg cagtcttctt tcttatcagt atgtgggcac
    39421 ccctgatgtc ttcaggttct ccttttctca tgggcgccac tacccttgca gtcaaaggat
    39481 acatcgctcc ccttctcaaa ataaaagatg acaggcaact ccttctgata tcaagactga
    39541 cgactcttat cattggaata atatctttgc tgctcggttt ttttgttaag caaatcctga
    39601 gagaagtgac atggatcgcg gttttcctga gcgcaatcgt ctacatcgtc tttattggat
    39661 ggtttggaat tcgggttgat agtaggtatg cctttatatc gcttatagga actttactta
    39721 ttgtcttatg ctctttcctt tttgatttac acaaaatcgt gcatccggtc tggcctgtaa
    39781 ccctctatgt ttttttagtt atgggtagtg gagcccttgt atctaaaaag cattagccca
    39841 atttttttct tcaatgatga tcagctcctc tccatcaaga ttaattttgt aaattttcag
    39901 gatttgtcgt atattacatc aatcaataaa aggaggttgt tatgtcggta aaaaatttgt
    39961 cagcactcct ggacatggcc atcaacaagg agattgaggc acaggaattt tataaggatc
    40021 ttgaaagccg tgtttcggac actgaagcta aacagacact gcaatttatg gcaaaggaag
    40081 aaaaaaaaca cgaggagttt cttgtcaaat ataaggaagc tggattgggc ccagaaactc
    40141 tgaaacagag ccaaatcatt gactataaga tagcagagca tcttgaaaaa cctgacatat
    40201 cagcaaagat ggacagcaaa gacgtatatc tggttgccgc tcacagggag cttaattctt
    40261 acaatttcta taaagagctt gctgaaattc accctaaggg atcaataagg gatatgcttc
    40321 tgaaaatggc cagcgaagaa atgaaacata aagaaaaggt tgagtatctg tattccaata
    40381 ctgcctttcc acaaacagca ggcgggtgag tctcgtatcg ctctataccg taacccatat
    40441 tctcatcgac gtattgaaat tcacatgaaa aacccggcac ctacagggcc gggtttttca
    40501 tgctttggca ctgacttatg ttacgtgggc gctattgaag aaagctcttt cttaaaggac
    40561 cagtaatata ctgcaccaat aaaaatgaaa ccacctacaa tgttaccaat ggtgacaggg
    40621 attaggttcc agtagataaa ctggccccac gtcacattgg cacccacaaa aataccagcg
    40681 ggaataaaat acatgtttgc aacgctgtgc tcgaatccag aggatacgaa ggccatgatc
    40741 gggaaccata caccaaaaaa cttgccaatc atattctttg aagaaatagc tagcaggatc
    40801 gctacgttta ccagaaggtt acaggcaatg cccttcataa aagatgacca aagccccata
    40861 ccgcctgctg ccatatatgg tagtgtcttc gcctcagcaa ttgcaatggc attctgacca
    40921 aatactgtta tctcagggcc accaccttta ctgaaaggcc ctactgacat cagataagcc
    40981 cagaagagcg aaccgatgaa gttcccaata tacacccaga accatgcgtt aaataccttg
    41041 ctccacgtac tctttttctg aaacactgcc atcggtatta acattgtatc tccggtgaac
    41101 agcgacattc cggtaaggac gatcgtaata agaccaacag ggaataccga tcctgaaatc
    41161 aaagatttga ttccaggtat tgtcgttcca gtggaacata ctgtggccag cgcacctcca
    41221 accgcaataa aaagacctgc catgaagctc cttacaagat aattgccagg ggtaagattt
    41281 gccttgtatt ttcctgcatc ccctgcgagt tgaacaattt caactggttt gtttgccata
    41341 cgccctcctt attttgataa taaactggta ctgctacggg tagacttttt caatcctaca
    41401 cgagcagacc ttgtattcag gtatcttgca aagcgggtca agggcatcat tggtcaactt
    41461 attggcagca gcttcaaaga agtgccatgg gaagaagaca acattaggtt ttgtcttctc
    41521 cgtgaccttc gtcttaaccc gcaagcttcc tctccttgaa gatattgata tcgtttctcc
    41581 atcctttatg ttgtagttct gcgcatcatt gggattcagt tcaacatatc cttctggcac
    41641 gttttcattg agtaccttgg agtttcgtga catggttccg gtgtgaaaat gatcgagcaa
    41701 ccttcctgtt gtcatgatta acggatattc ggcgtctgtt tcctcagcag gagacgtata
    41761 ttcaatgggg gttaaaacgc caagtcctcg cgaaaatctg tcccgatgaa ggaactgggt
    41821 tcctgggtga tcctcagtcg ggcagggcca ctgcagtaac tctttcctga ttctttcata
    41881 tgtcattcca gcgtaggacg gtgttacttt tcgtatttca gcaaagatat ccttcggctt
    41941 tttataattc caattcaccc ccattcgctt tgccacttct gtaataatcc accagtcgtc
    42001 cttcgcttgg ccaggtggag aaaccacctt gttcatcatc tgaacccttc tctcagtgtt
    42061 agatacagtt ccgctctttt caagaaaggc acaggcaggg aggactacat gggccaattc
    42121 tgcagtttct gtaagaaaga tgtcttggac aacaacaaac tcaagcgctt tgagcgcatc
    42181 gatgacatgt tgctggttcg gatcagagat aacagggttt tctcccataa tccacaacgc
    42241 cttaaaatct cctgcaagtg cgtgattgaa catctccatg agtgtaagtc ccggtttcaa
    42301 tgaaagattt gatgtgcccc aggccatttc aaattttgat ttgctttctt cagttgtaac
    42361 cggctggtat gcaggataga cattggggag accacccata tcacatgcgc cctggacatt
    42421 gttctgaccc cgtaatggat ttaccccacc accggccaca ccaacgttcc caaggagcat
    42481 ctggaggttt gcagtagatt taacaccatt aaccccagac acaaactggg ttatacccat
    42541 ggaataatac aaggccatgg gcttgagagt tgccatgaga tgagcggcag ccacaatatc
    42601 atccgggttg tttaggccac agatttttgc gacataatcg ggagtatatt tctctaaact
    42661 cttagcgaga tctgcaaacc cttcacatct cttttcaaca tattcattgt catacaaacc
    42721 ttccttgact ataacatgca taaacccatt aagaatcgct atgttagtgc caggattgat
    42781 tgcaaaataa tagtctgcat actttgataa ttctatgttc cgcggatcta caacgatcag
    42841 tttcttttta ttattaagga cctccttctt gatcattgac ccgattacag ggtgattttc
    42901 tgttgtgtta gatccaataa caagaaaccc actggaagtt ggaatttctc gaatagagtt
    42961 tgtcatagca cctgaaccaa aaacagccgc cagaccggcg acggttgagc tatgtcagag
    43021 acgggcgcaa tgatctgtat tgttcgtgcc tataactgcc ctggtaaact tttgcatgag
    43081 gtaattctct tcattagtac acttggctga acaaagggtg ccgatggcat cagcccctga
    43141 ttttttctta acatcgctaa gtttttcagc cacaagattg agtgcttcat cccagctcgc
    43201 ttctacaaaa ttaccatttt tcttaatcaa cggtttggta aggcgctttg ggctctgtac
    43261 gaaatcccag ccgaatcttc ctttcacgca aatgtgtccc cggtttggtc cgtcatcatt
    43321 cccaatggcc ttaacgagaa caccttcctt attcttatag aaatcgatct ggcatccaac
    43381 accacagtaa atacagatgc tcgtttcctt tgtaagttcc cagttttggc ctgttcccaa
    43441 gactgtctga aacgagagag ctcctgtggg acacatttgt acacactggc cacattttac
    43501 acaactagat tcaccaatcc gcttgttgtc atcagcaatt aagtgtgtct cactgcctcg
    43561 ctcacacgtc ccccataccc gacacatctg aatctcgtta catgtcttga tacacctttg
    43621 acacaagata catctcattt catttctctt gatagcgttg ctggaagtaa aatcgatttc
    43681 acggtcatga cggatacttg tatctgtgac aggacctact tgatactttt gtactagggt
    43741 ctgcagttca caatctccat ttgattcgca ataaaggcag ttatggtctc cctccatgag
    43801 aaggagttca agaacaaatt ttctatttgc aattatgccc tcatcttctg tgacaactgt
    43861 catatcatcc ataatcggtt cagtgcatgc cgtcctcagc attctgtcat tcacccggac
    43921 aatacagata cggcaggcgc cggtcttact tatttttgga tggtaacaaa gggttgggat
    43981 atagatgcca ttctctgtgg caacttccaa aattgtctgt cccctccttc cttttactac
    44041 gcgtccatca atactgaatt tgattaaatc tgaatccatg actaaaaccc ctcctctctg
    44101 agctctgcct gttatttatt aacactgtgt tcttaaatcc tgtaccttgt gccgccagaa
    44161 tttattcagt gattctaatg attaacaaaa ttgttacagc cttatttttt catatccaac
    44221 aatatcacta acatgaattt atacataaga gggcgcagct tttacgatgc aaaaccctgt
    44281 aatcggtgta tgcatctaat gtcattcaat gccttaaatg acatttgttc accctgatat
    44341 accaattata caaagactaa ctatctctgg aaattattgc aagaaaaaaa tttttaatcg
    44401 ttaaatagaa aatcttatta tcacacgtat gttaggttga ccagtttccc tatttttttc
    44461 agcttatctg caagaagttt tacgattacc tcgcggggga aaatacccat ctgacgaaaa
    44521 acaatatttt catgccctgg attagatgcg gttcccacaa caaaatttat cgtgtccgca
    44581 gaatgaaaca atcggtaaag atctgataca gaggaatctg tttctagctt atcaggattt
    44641 tcttccagta tattatgaac ctgatttaag catatggcac cttccgtggc ataatcgatc
    44701 cctttaatct cgtaagatgg cggtttgtgg taagcactcg gtattgtttt catgtcaata
    44761 ggcacgccaa gtatctttgc taccatttca gaagtcgttg aaccacatat caccttggtt
    44821 cccttcatcg ataagaagtc ttcaactatt tttttatcat tattcttgtc agccggcgga
    44881 ccggtaagaa tattaagtgt cttcccttct ctgcaaaaaa ttgccacaca agtcgtgtcg
    44941 tccccgtatg tggcccctga tatttcttta accttttcca gcactttgtt tggtatcatc
    45001 ttcagttcat acccctgagc aagacagccg ttgataaaat cgcacgctcc ctgcacactc
    45061 caccccatcc tgtattggtg gcctaagcca gcctggctca caccatcgct taccagaaaa
    45121 actccgtctc catagttgag catacaattg acctcagcaa tcatctcaag accaagggga
    45181 taaaaacgtt gcttcggtaa ataagcagca agccggttgt tgatcaggat gggggaaggt
    45241 atttcatagg ccataatagt tgcatgtcca ttgtttaaaa cccgacaggc tgaaaaagca
    45301 gaaaaaggaa tatccttggt acgggcttcg tgcattgtat gaacgatttt actgcaagcc
    45361 tctctgagag tgaagcctga ccttgtaagc tctaccagcc gtgaggcaca cataactgca
    45421 gctacgcggg cctttattcc ggttccaatc ccatcggcaa cgacggttaa tgtcgcctcc
    45481 gggctccttt cagtgacaac atagtcgcca cagaccctgc ctgttctctt tgcactctgc
    45541 gcttctacca cttccatata ttttctggga tcactcagac ttggtttcat caccctcact
    45601 taaactcata agctttctaa ccagttcttc tccccttgca gtgctttccc ctagaaacag
    45661 agccatctgc tgtgccattt gaatttggtg ttcgaggagt tcatgtgctt gagcaactgt
    45721 ctgtgatttc atctcgcgca gtttctttac ctgctcctct tcgcttgtga tatttacgaa
    45781 gactcctacg atctgttgtt cctgcttcag gatatacaat aactctctac ataacagatt
    45841 gtaaggcctg tgaaaaacag taatatcaag actttcggca gctcccgtga taagtttttc
    45901 gaaaggtgcc ggatccataa gatatgatat gtgccgcccg agaatagcat ccgaacagga
    45961 aaagtatttc ttgaaggccg ggttgatacc gattatgttc agttccaaat caagcattag
    46021 gatgccgttg ggagtggtat taaataactg atcagtgcga cgctcggcaa gtctgcgcat
    46081 ataaggaata cacatttcag gttctgccat accttgtgcc accgcaattg ccttttctcg
    46141 gcaactttca tatccgcatg ccccacagtt aagctgttgt tggagatctg attttcctgt
    46201 acgttcaaga atcccctgta tttcctcctc ccccacttct tttccgattt tttcttcttt
    46261 tttgtacgat gcctttacta tcttttcgtc ataaatctct ggaaccacgt cgtttcctgc
    46321 tatacttcta tcatactgaa taatatcttt ccgtcgttca aagacattct taatagaact
    46381 tattcctggg ccatttatac acccctgact gcaaaaaaga ggttcgataa gtgtatatga
    46441 ggattctttt ggaatgttgt gaaatatttc cttcacattc tgaaatccat ctacccttac
    46501 cagcttcgtg tccagaccat catctttcag acctgctgtt ttgattaatc cacctggaag
    46561 gggatataac tgagcaaatc ttacaggctt ttcatcaaaa ttactttctt cacagttgct
    46621 aagatcgatc cccctttgct ccagccaaac catcagttct ttgaaggtaa gtacacagtc
    46681 tattgcatca accacatcag acctatataa ctcggttttt tttgcaacac aggggccgat
    46741 gaaaataacc ttagactcca ttccgaatct atcctttagc attcttccat gggttgccat
    46801 gggtgaaaga atgggcaaaa gattgtccac aagctcttgt tggtattttt ctatgtaatt
    46861 caccagtgct ggacaggctg tggcaatata tggtttggat ccatcttttt ctatcacttt
    46921 agaagagtgt gctgacacct ggtatgcacc gtgtgacgtt tgaccaataa aacggaatcc
    46981 aagtgcacga agcgccgccg gaagcctcat tccttgccat ccactaaata ccgccgcaaa
    47041 tgatggggct atgctcgctg acacaaatgc accactttca atcagttttt gtgctgtatt
    47101 gatatcataa cggaaagttt ttgcctgctg tggacattcc cgtatgcaag tcccgcaggc
    47161 aatacatctc gtttcatcaa cataggcctg accgttctgc attcgtattg cctttacagg
    47221 gcaattcctc agacaacgat agcaatctct gcaccttgct gttaaagtga agacaatctg
    47281 gcggttgtcg atatcatgca tttcgctgta cctcttttaa tgttgatgct gccattccct
    47341 caatggcttc tcgtgctttg tccaatgtac agtgttcaat aattttgtcg ccaattttta
    47401 ctacagggcc tctatcgcag gtttcaaaac aaaatgacgc tcccaccttt acgcttgaat
    47461 ggagtccgtt agaacggata tattccagga tatcccgaag taatttctgg gaacccttca
    47521 ggaaacagcc cgtcccgaag caaacgttta cctcaaaacc ttgatcatca ccagaggaga
    47581 aggtcatgtc ttcatctgaa attctcttac gcgtcttgta acttgtatgg agaagatgat
    47641 gtgccttctc gccaccaatg tccccaagat atgaggaata gagttctgtc acgtaaggat
    47701 tatcctgaga cttatgcaac tccagcatcc tatcattttc gtaaattcct tttgtcctct
    47761 tgtgtctgat cgaaggattg ctataaaccg gttggccagc accgccaaca catccacctg
    47821 ggcaggccat cacttcgaca aaatcatagt gtacatcacc tgacttgatt cgctcaagaa
    47881 ggtttctcgc attgccaagt ccatttacaa cggcaagaga aaagtccttg gacccaactg
    47941 taagggttgc ctcacgcatg ccattgtcgc cacgcacggt cgtgaattca taactatctc
    48001 tcttttctcc tgtaattttc tctgtaatat aacgtaaggc tgcttcactc acaccacctg
    48061 agttcccgaa gatcacaccg gcaccagttt tgaatccaaa gggaagatga aatgattcag
    48121 gttccagttc cctaaagttg attccggcct cttcaatcat ccgaccaagt tcctgcgtgg
    48181 taagaacatt atcaacatct ctcataccat tatggttaaa ttctggtctt tttgcctcaa
    48241 acttttttgc ggtacaaggc attattgaca cgacaacaac atcttccggt ccaattccaa
    48301 gatcatttgc caaaatctcc tttgcaagcg ctccaaacat ctgttggggt gaacgacagg
    48361 tagaaagatg attcacgaat tctggatagt actgttctac aaactttacc catgccggac
    48421 aacatgatgt aaactggggt atattttcat ttttctgaat tcgattaata aattcattgc
    48481 tctcttcaat tacggtaagg tcagctgaaa aagatgtgtc aaagattttg tcaaagccta
    48541 ttgcctttaa tgcagcagca atctgcccag tggttgtcac acctggttct aaaccaaaca
    48601 tttcaccgag ggcaacacgg acagccggag ccacttgacc aacaaccact ttattcgggt
    48661 catatatcgc cttccaaacc tcctctattt cggatttggg cataagtgca ccagtagggc
    48721 aaacccttgc gcattgccca caatttatgc attccacttt atcgagttct ttcccgaagc
    48781 ttgggattac tacggtatga gcacctcgat aagcaaaatc aatggcgccc acggactgaa
    48841 cctcagaaca gatgcgcaca cagtcaccac aaagcacgca cttattcggg tccctcacaa
    48901 gtgagggcga tgagtcatca aggggcagtt cccgattctg ttttttgaac cgaaccttag
    48961 agacccctaa tcttcgtgcc aatgcctgta gctggcatgt agaacctttc tgacatgtag
    49021 gacagtcttg gttgtggttc gccagaagga gttcaacaat aatttttctc attttccgta
    49081 tttcttcggt attggttctc actttcatcc ccattgcagg cggggttgaa caagcaggta
    49141 caagacccaa cccctcaatt tcaaccatac acaacctgca tgacccgtac acgcttaatt
    49201 cagaatgata gcagaacgtg ggcaattcaa tgtttgcttt tctgactagt tccaggagat
    49261 ttccttcctc ttctatgagg attgttttat tatcgattgt cacatagcgc tcttcgctca
    49321 tgattttcct ccttttatcg cctgaaattt gcagacctct atgcaagcac cgcactttct
    49381 gcagagcatc tggtctatct tatgaggttg tcctttttct cctataatgg cgcctattgc
    49441 gcattttgtt acacatagag tgcatcccct gcacaagttt tcatcaatcc agaatttagt
    49501 caactttttg catttaccag ttggacaata tttatgtttt acgtgtgctt catattcatc
    49561 acgaaaataa cgtaacgtag aaagcacagg gtttggggca gtttttccaa gaccacaaag
    49621 ggaacctttt tgcactgcga tggcaaggtc ttcaagaatt gtaagtgttt cttcagtcgc
    49681 cctcccctcg atgatgtcat cgagaagcga cagcatttgc ttggttccct ccctgcaaag
    49741 aacacactta ccacatgatt cttcctgtgt aaaacgcata aaaaagcggg caacgctaac
    49801 catacaggta ttcttgttca ttaccacaag cccaccagac ccgatcatag ccccaacctg
    49861 ttttagagaa tcgaaatcga gagggaggtc gaggtggtca cgggtaagac aacctcctga
    49921 tggtcctcca atttgaactg ctttgaatcc gtcattcatc ggtttgccat cgtcagacgt
    49981 catccctcca gccacatgaa acacaatctc tctgagggta gaaccaaatg gcatctcgat
    50041 gaggccggtg tttgcaacat gtcctgtaac agcaaaggtt tttgtccccg gtgattcatg
    50101 agttcctaag gaacgatatt gatccaagcc tgcagcgagc acaaggggaa tgttagacag
    50161 tgtctctaca ttattgatta cagtcggctt gccccaaagg cccctctgtg ttggaaaagg
    50221 tggtttaggc gaaggcatgc ctcgtctacc ttcaatggat gcgatcattg ctgtttcctc
    50281 accacataca aatgctcccg caccttccat aacatgtaaa tcaaaattat atgaggaacc
    50341 gaatatttgt ctacccaaca ttcctgcttt gtatgcatct tggatggcct ttcgtatcct
    50401 ttttactgca agtggatatt ctgcccggac ataaacatat ccttcatcag caccaatcgc
    50461 ccttgcagct ataatcaaac cctcgacgac actgtgagga ttaccttcca taacgcccat
    50521 gtccataaat gcaccaggat caccctcgtc accgttacat atgacatact tcttgtcact
    50581 ttccttctgt aatgccatct cccatttttt tcctgtaggg aaaccgccgc cgcctctacc
    50641 acggacacca gcgtcaccta ttcgtttaca aacctcaagt ggtgtcattt caagacacgc
    50701 ctttgctgca gaaacgtatc caccgtgtgc aacatattca cgcaaatctt ccggatcaat
    50761 gacaccacag ttagcaagca caaacctttg ttgttttctg taaaatggaa tctcgctctg
    50821 atttttacaa ggtttctttg tgtcaggatc gacatacagt attcggttaa gtaattctcc
    50881 tcgtatcagc gtcgtctcta caatatcttg cacatcatcg ggccttacct ttgtataaag
    50941 tatattttca ggaaggattg taaccagtgg gcctatttgg caaaatccct ggcagccact
    51001 tttggaaaca aatacaccat cctcttcttt ctcttttttg agctcaacaa ctgcattgag
    51061 accggagtct tttatacatc ttacaaattc ctcgtacact ttcattgaac cattgacgat
    51121 gcaaccagtt cctgcacaga cgattattct tcttgccaaa actgtttgtg ctgctaaata
    51181 atcttctttg attttattaa gccccatgag tgtcaaattc atttttctcc tccttatcca
    51241 aaatatcatc tataatagag tccactcttt caggagtaag ctgtccaaat acctcatcgt
    51301 ttatcaccat tgcaggggca agtccgcatg caccgagaca attcactgtc tctagggtaa
    51361 aaagcatgtc aggggtgctg tctgctcctt ccttgagagt aagcttgttg cggagtgctt
    51421 cgtagagatt catagaacct tttacatggc atgctgtccc atcacatatt cttatgatgt
    51481 atttcccttt cggtttcata gtaaaaaggg aatagaatgt tgccacacca aaaacgcttg
    51541 caggggaaat acccagggat gtggcaatgt acgtaagaat ctcttcagga agatacctat
    51601 attccgattg tactgcctga agtattggga tcagccgcgc agcactccga tcattttgat
    51661 caactatttc acagaccttt tcaaacttcc tcaacgtttc catacttttc acctcgctat
    51721 ttatctgttc tcattgacac tgcaagtttt tttgataaaa caactaagga tgatgcaaga
    51781 ttttcatgct gtacaatgat ttcggctggt accatgatct taactggcga aaaattccaa
    51841 attgcatgta taccagcgtc caccataaga ttggccagat cctgagcaca gtatgaaggt
    51901 acagtaagaa tacctatttt tatgcccatc ctcttgatca tatcaggtag cttttttaca
    51961 ggaaaaacca tctttccatg tatttctatg ccagtttttg cttcatctga atcaaaacct
    52021 gcgatgatat tcaagccata ttctttaaaa ccctgataac ccaaaagcgc tgaaccaagg
    52081 tgaccaactc cgacaaggta tgcatcggtc gtgttattcc aacccagaaa gtcttcgatg
    52141 ttggacagca aatgttttac cctgtatccc agttttggtt tgccagacgc ctcagttatc
    52201 gccaggtctt tgcgcacctg aatgggcaga atattaagtt cattaccaat ctgagtacaa
    52261 gaaatatgtt catgcttctt ttcagcctga atttgcttta aatattgata atatatgggg
    52321 agtcttctga gtgtaggttc tggtatagct tttagggcca tctttatctt gtgattagaa
    52381 ccattcatta aatcccctcc ttcataattg attatttttt tatcgatata attttatcga
    52441 tataatttta tcttgtcaag gattttaaat atatagtagg taaactttct gcttgcataa
    52501 cacagcatgc caatctatac tctttttact catcaacaat gaaattattt gacgtacaaa
    52561 aaatccttga ggcagaagtt ctttgtggag atgaccttct acacaaagat gttcacaact
    52621 ggtttgcatg cgatcttatc aacgagatgc ttctttatgt tacgccggac acacttcttt
    52681 taacctcatt aaccaatatc catatcgtcc acacagcaca ggttatggac gcaatcgggg
    52741 ttgtgtttgt ggggggtaaa agacctgctg agtcagtaat cacgaacagc aagctctaca
    52801 acattcctct cctgacaacc aaacatctta cctttgagtg ttgtggccgc ctttttttaa
    52861 ataatttgca tgaccctacc gtggtcaagg atgctaccaa tgcctacaga taatctccaa
    52921 tttacacccc acattaaact tgaattcaag ataaacgaga aagatttctt tgtagcaggc
    52981 gaatcagcat cgagagttaa aaaaacgctt caacaattag gcctccatca ggatataata
    53041 aaaaaagttg ccattattat ttatgaagct gctatgaatg ttgctatcca cggaaatcat
    53101 ggcatgctca ggatttatat cgatcctgaa tctattttag ttgaaacaga tgacgaaggg
    53161 gacggaatac ctgacattga acttgctatg caggaaggct attccactgc cactcacgag
    53221 attagggaaa tgggattcgg tgctggcatg ggccttttta atattgacca gtgctctgat
    53281 gaacttcata ttgattccaa ggttggtact ggcactaaac tacaggcaaa agtgtatctc
    53341 agaaagaagg acagcatttc agatccttga agataaaggt gaatgattcg taattttcat
    53401 tctgtcaggc tcgatcctga aaagtgcaaa ggatgtacaa actgtataaa aagatgtcca
    53461 acagaggcta tccgcgtcca tgatggtaaa gccacaatca tcgaagaacg ctgcatagat
    53521 tgcggtgagt gtattcgtgc ctgtcctaac catgcaaaat ttgctgtcac agacgaatta
    53581 gacaaactcg ctgcttataa gtacaacatt gcactccctg ctccatcatt tttcggccag
    53641 tttaagaacc aggaacatat tgaagatatc ttgcatgcct tccttaggct gggatttgat
    53701 gacgttttcg aagttgccct tgccgcagag attgttgcag atattgttca ccggcatctg
    53761 ctgaatgatc gcgtcaaaaa acctttgttt tcctctgcat gccctgctgt actccgcctg
    53821 atgcaaatca aatttcctga cctcctccac caatgcgcac ctgtccttac cccgatggag
    53881 gttgctgcac ggttagcaaa agaggaagct gttaaaaaaa ctggcttttc ttatgaggag
    53941 atcggtacat tctttatatc tccatgtcct gcaaaagtga ccgaggtgaa acacccgatc
    54001 gctactaaat cctcagcagt ggacggggta atcggtgcta atattatctt taaggatatc
    54061 cgaaaaggga tttttagacc tgatggtatt agcagatcat ccaaactaca aagggcaaca
    54121 agtcttggca tggcatgggg ctttgtgacc ggtgaatcaa agaacgtaaa ctctgggaga
    54181 gcacttgcag tgagtggaat tcataatgta ataagtcttc ttgatgaagt tgaacgcggt
    54241 gagcttcagg atgtcgattt tttggaattg caggcttgta ctggagggtg tgttggtggc
    54301 cctttaaatg ttaccaatct ttttgtaggg agggttaggc ttcgggacct aatcaaccga
    54361 tttggttcaa aagagccctt cttcaataag aataaactca ttgagttcta tgatcacggt
    54421 cagtttgatg caactgaacc gttccaacca agattcctca tgccccttga tgaagatgtt
    54481 agtcaagccc ttattaagat ggagcgtttg gatcaaatca cgaatgattt accggggctc
    54541 gattgtggag cctgtggctc tccgagttgc agagcttttg cagaagatgt gataagaggg
    54601 attgctattg aaactgactg tgtcatcaag ctcaaggaaa aagtaaagat gctttcagac
    54661 ggtatccttg atcttgcaag ggttatacct tcagcatcat ctgataatcc aaagcaagaa
    54721 taaagacctt atacgag
//
LOCUS       DAOOMC010000003        47634 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdus sp. isolate AMR_MDS_2685
            NODE_532_length_47634_cov_6.216944, whole genome shotgun sequence.
ACCESSION   DAOOMC010000003 DAOOMC010000000
VERSION     DAOOMC010000003.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864760
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdus sp. (wastewater metagenome)
  ORGANISM  Syntrophorhabdus sp.
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae; Syntrophorhabdus.
REFERENCE   1  (bases 1 to 47634)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 47634)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,729
            Genes (coding)                    :: 2,708
            CDSs (with protein)               :: 2,708
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 7 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47634
                     /organism="Syntrophorhabdus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_532_length_47634_cov_6.216944"
                     /isolate="AMR_MDS_2685"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2699756"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            777..1967
                     /locus_tag="PK125_00560"
     CDS             777..1967
                     /locus_tag="PK125_00560"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPB36631.1"
                     /translation="MRQEAVMMRWVILAQLCLCMLSYALIFQSIPPIMSLVISQFKLS
                     HYQAGLLMSLFALPGILVSLPAGMVADRYGVKPVGIFSFLLALLGSFLVANGPSFEML
                     LVGRILAGIGASSLVITIPQAIAQWFSDRHMGIAMGIYNTAMPIGTIISLNGLSHMAG
                     VWGWRAAVWATVFFTAIIFVIFAVFYRQPDHKDRLENQGKKDQNREGIGTSIFFVGFS
                     WALFNASIISLFTFAPDFLVSRGMTLDHAGFSTSLVMIASMCLSPIVGLIIDKIGQKE
                     LFIAAGGLGMAVFIALIPGIGGAFSLFLLCVGVASALIPVSVFSLAADVVSFQRLGLG
                     YGILSMLNNVGMFVGPQAAGLGRDLTGSYQISFLVMAFFAFFCTITILSLWLKRRGAS
                     SGNR"
     gene            2240..2491
                     /locus_tag="PK125_00565"
     CDS             2240..2491
                     /locus_tag="PK125_00565"
                     /inference="COORDINATES: protein motif:HMM:NF018500.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheU family protein"
                     /protein_id="HPB36632.1"
                     /translation="MSVHIIPVNRLSPEALRGVIQEFVSREGTDYGEREVSPEAKFEQ
                     VRYKLEHGLAVLIFDDETQTTNILSADDPILRRINGFAE"
     gene            2923..3333
                     /locus_tag="PK125_00570"
     CDS             2923..3333
                     /locus_tag="PK125_00570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB36633.1"
                     /translation="MVGPEQIVPEEIRDRKKALDFVNIVKALRVLVSKEGFPYDRINK
                     LLGMVQRHGDCSCENCQEVRKKETLRFNRSLSKIARFRREYYDAGRDGEFEEIIKIAR
                     KGAELLFLLQRAGLDDVELIRATIKIYRKKIIFV"
     gene            complement(3626..3719)
                     /locus_tag="PK125_00575"
     tRNA            complement(3626..3719)
                     /locus_tag="PK125_00575"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3682..3684),aa:Ser,seq:gct)
     gene            3949..5160
                     /locus_tag="PK125_00580"
     CDS             3949..5160
                     /locus_tag="PK125_00580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB36634.1"
                     /translation="MNKTMGNMRNIITGAIIICLVSIVGLVGCDDKKMSPIAANEVSA
                     ETKIRQGTQVKGDTQPIGNAQFFVTYRYTDPMTGMEAFRLLIPKGWQAQGQIAWSANP
                     ALPAQARFRFWNPNGSGEFNLFPTQAYFWTNNRLFLSTNPPGSLRFNTLVMRPVDLQT
                     AFTKIVIPYARKGVSGMKMIKASDVPELANIAKGQPVQGVRSFANGGKMRIEYQENGR
                     PMEEEIYAAVSQFVTDLPGSALGPGYFINYWYIDYVFSFKARKNELDSQSKVFQTMVY
                     SLRVNPRWFAKVANVKEMMVQKIIKGIKDVGRIGEMVARAGSEMREGQMKDWERRQQA
                     NDRIVQNFTDHIRGVERFNDPYAGKEVELPTGYGRAFSNNLGEYIVTDSPSYNPNIGS
                     NVTWQELKPVK"
     gene            5222..5767
                     /locus_tag="PK125_00585"
     CDS             5222..5767
                     /locus_tag="PK125_00585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB36635.1"
                     /translation="MKTKLFLITILVSIAVSTVMFLGLTASLTAPSSSMKTQEGIGSI
                     LDIPANWSMGTFPSGMSYFAAADGKRVTRDADGTNVSVLLGLAAGYRKTNFQNARDAA
                     DQTIRFYQSDNPGLRVILREAAKLGGFPPESVLIESSTGRDGEMERSLMLITAREGQM
                     FMAAFTSPANDYNSLQRNLSK"
     gene            complement(5876..8059)
                     /gene="pbpC"
                     /locus_tag="PK125_00590"
     CDS             complement(5876..8059)
                     /gene="pbpC"
                     /locus_tag="PK125_00590"
                     /inference="COORDINATES: protein motif:HMM:TIGR02073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 1C"
                     /protein_id="HPB36636.1"
                     /translation="MQKWWFTHNNRSIRRKSTIKMRIVLAALVLIIAPGLLWVLIDIY
                     RSANGRPCASFEEIKQKYAVSDAALLDRNGEVIHTLRVDVHGRRLDWTNLGQVSPSMV
                     RATLRVEDKRFYSHGGIDWLALVSATFSNLTHKNLRGASTITMQLTSILDRKLRPRNG
                     SRRGILQKWNQLKAAVSLDNHWTKDQILEAYLNLITYRGELSGICAASRGLFNKEPSG
                     LEEAESLILASLITSPNASVEEVAKRACRFASPVSRNSIRDMAYKTLGRTYHIRRDAS
                     IAPHVARLLLTNGKKESKCTLDGNLQRYVHASLNEAVRFLENQNVSDGTALVVENKTG
                     DILAYVGNSGTSPTTLYVDGITAYRQAGSTLKPFLYELALEKKLLTPSSILEDSPLEI
                     VTPTGLYVPHNYDNIFRGPVSVRTALSSSMNIPAVRVLGLVGVTDFVERLQELGFKGI
                     SQAPEFYGYSIALGSADITLYELVNAYRTLANNGRFSNMRLVFDGNRHHAQNILDKKA
                     TFLISHILSDRQARSTTFGLENHLSTRFWTAVKTGTSKDMRDNWCVGYSDTYTVGVWI
                     GNFSGEPMRNVTGITGAAPVWLGIMNYLHRGFASKPPQPPGGVQSAQLTFEDSIEPPR
                     TEWFIAGTEPAGMVLVNRVHAKPRITYPTQETLIAIDPEIPQHLQRVPFRFEPQSRRF
                     TWTLNKEKIGTNDSVYLWTPKLGVHKLAITDEDGHILDSVTFLVR"
     gene            complement(8031..10622)
                     /locus_tag="PK125_00595"
     CDS             complement(8031..10622)
                     /locus_tag="PK125_00595"
                     /inference="COORDINATES: protein motif:HMM:NF036531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
                     /protein_id="HPB36637.1"
                     /translation="MPFREATALITIEREGVIDTSIQRLTRNNPVIEVPVKNSYAPNI
                     FISAFIVRGRVADAKPSVMFDPGKPAYKMGITELRVGWQKHELKVKITTDKKTYPVRQ
                     VVDVKIKVTTAFGKSPPEAGEVTIAAVDEGLLELKGNESWKLLEAMMARRPYEINTST
                     AQMMVVGKRHFGRKSLSYGGGGGKQTTRELFDTLLLWKSSVPLNDNGEATVKIPLNDS
                     LTSFRIVAIASAGASFFGTGSATVNTTQDLMVISGIPPLAREGDRFFARFTIRNTSDR
                     LMDIRALLSTTDLKERKDLKPLDMKLPAGESREIGWDVTVPAGTEKLLYEARAMDKIE
                     NVTDTVKITQKVAPTVPLRTFQATLAQLKEQHRIAVQFPPDAVPSRGGIRVSIKPKLA
                     EGLGSVTEYMKDYSYTCFEQKVSRAIALRDKKIWGSLMNDLPSYLDQDGLIKYFPVRF
                     ISGTDTLTAYILSIAHEAGYQIPKIPKEKMLEGLKGFVQGRVVRWSNLPTADLSIRKL
                     SALEALSRYDEAQANLLESVTIEPDLWPTSAVIDWIGILSRVTDIPDRLGKMKKAQSI
                     LRSRLILQGTAMNFSTERSDYCWWLMVSTDTNAIKTLLTALQFDSWNVDSPGIARGVV
                     GRLRHGRWNTTVANAWGIIAMDKFSKKFESLPVGGITWSTLVNKTVATDWVKTPNGNE
                     SFFSWPQKNEEIFIKHEGTGKPWVTIQSIAAIPLKESLASGFKIKKTITPIEQKAAGK
                     WTRGDVVRVNLEIDSQSDMTWVVVNDPIPAGSTILGGGLGRDSSILTKQETGKGLVQE
                     VFRERSFESMRAYFEYIARGKFTIEYTMRLNNEGAFNLPQTRVEALYQPEMFGELPNA
                     KMVVHPQ"
     gene            complement(10625..13867)
                     /locus_tag="PK125_00600"
     CDS             complement(10625..13867)
                     /locus_tag="PK125_00600"
                     /inference="COORDINATES: protein motif:HMM:NF023400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MG2 domain-containing protein"
                     /protein_id="HPB36638.1"
                     /translation="MVKRQLFWPFLFALFSIACWTVIADAATIEFFSPEGTAKKVDQV
                     KVRFSDQMVAFGDPQLTGPFDISCSEKGTGRWIDGKNWSYDFDRDLPSGVSCVFRLKK
                     GLKSLAGKSIVGQTSFRFNTGGPSVITAQPYEGDEYIDEQQRFVFFLDGDPDDTSLTQ
                     NVYCSIEGIKERVGIRLLTGSEKNDFLKSIGHDKDHAPVTVFDCRQQFPSQARIDIIW
                     GKGVKSKTGIATTKDQKISYKTRTPFSVTFRCMKEDPKANCMPLSPMNLEFSSPVPWN
                     FVRDITLKNEKGQQWKPSKIEYSHAIAYLVSSVKKPETDEKRKDLTVQSISFEGPFPE
                     NTPFTIDIPRGLKDDTGRRLTNEKKFPLKVMTHSYPPLAKFSGHFGIIEHTDETSLPV
                     TVRNIEETIQAWAYQTGLPEKGATDQSKTDRGKYLNLTQEVKGKVRDLGADNEEVIIE
                     WLKRLRFTDRRESVLKGTGQETTFTVPKPGPSKEFEVIGIPLKDKGFYVVELESKLLG
                     ERLLGKPQPIHIPTSVLVSNMVAHFKWGRESSLVWVTSLDKAQPVDAAHVKLRDCSGN
                     VIWRGKTDRDGIAKIGRALPSKDKLTKCPVERKDNQDNWTNFEDSQLLSGITSGIFVF
                     ASKGNDLTFTHSSWTGGIDPWRFNLPTRYDNGDKGNTITHTVFDRQLFRSGETVSMKH
                     FIREQDMTHGITFPTGKKLPDMLIIQHVGTDQEYSFPLQWTARGTAETTWKIPENAKL
                     GTYRTFLLQKGDKSVRQKVEWNTGSFEVQEFRIPLMRASVKGPKEPVINAKDVDVDIS
                     LTYLSGGGASFAPVKLRSEIRPRFINFEDYSEYMFSNGYAKKSLTKPLPVHEEDGPQE
                     DSFVSPGRNGRSDGQEIKLKTHDLTLDATGSARLNLSDLPEIDTPRDILSELEFKDPN
                     GETQTVSSRIGLYPSRIHAGITIVKSGRIEDPLVYKVIAVDLKGKPVPGAETVVSLLK
                     KNIFTHRRRVAGGFYSYKSTTELVEAGFHCQGKTDAQGILTCEGKPPVEGEIILQAEV
                     RDSAGNISTAYTETSIYGREDRWFEMGDDDRIDLVPEKKQYNQEKRHAFR"
     gene            14037..15020
                     /gene="epmA"
                     /locus_tag="PK125_00605"
     CDS             14037..15020
                     /gene="epmA"
                     /locus_tag="PK125_00605"
                     /inference="COORDINATES: protein motif:HMM:NF006828.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P lysine aminoacylase EpmA"
                     /protein_id="HPB36639.1"
                     /translation="MMCNKSQQSTLNPQLALLQFRHKLIKALRDFFYERNYLEVETPN
                     LMTTVAPDPNIDALKVFIDDRGPFFLHTSPEMGMKKILLHGHKRIFQICKVYRVEEQA
                     DIHNTEFTMLEWYREGTYLDAMKEVEELISCVAGMLTIDNREVFARPWKIYELSELFM
                     TMTHINPFGLTRDELFASMESLGFPGIDERDAWNDLFFKLFIQEVEPKIPQNVPYFIM
                     DWPLSVSTMAKRKDENRVERFELYIKGLEIANGYSELLDVSEQRKRFEQDNLERKASG
                     KQTFVIDGGFIDALNGIKGPCTGVSIGVDRLIMVLLGKERIEDVLINRIRL"
     gene            complement(15582..16487)
                     /locus_tag="PK125_00610"
     CDS             complement(15582..16487)
                     /locus_tag="PK125_00610"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HPB36640.1"
                     /translation="MLVKELCIKKFFALAIVSAFLFISCQTVTTAPAIIRKGDPKIAL
                     VLGGGSAKGFAHVGVIRALEQEKIPIHMIVGTSVGSLIGAIYASNPDSFELEWKAFKI
                     ERNDVLDPSLIYSKFGPVQGVRLENFVDQTVKTKRIEDTRIPFYPIATDLNTGETIVL
                     EKGSVAKAVRASSAIPGIFVPVTFDNRVLVDGGVTNNIACDVAKSKGADIVIAVNISR
                     DIREANINSLIDIVGQSAAIMMHEASKNKLQHADIVIEPNTKGVGMFDFTQKKFLMDE
                     GIKAAKQAIPRIRDLIARYKSGTPS"
     gene            complement(16462..17106)
                     /locus_tag="PK125_00615"
     CDS             complement(16462..17106)
                     /locus_tag="PK125_00615"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HPB36641.1"
                     /translation="MFVKQMQVGGFAVFAYLVACKITNEALVIDPAAEEDRIFKEAES
                     RGYTIKYIVNTHSHIDHIMGNRRMKELTGADIIIHEKEAGALVQQSPDMMLMFHAKPS
                     PPADITVRDGDFITIGKTSLKVLNTPGHSPGSICLYHNGMVFTGDTLFVGGVGRTDLG
                     GGSLQQLVSSIRNKLFVLPDDTIVAPGHNYGDTPKSTIGREKLYNPYVGERAVY"
     gene            17232..17936
                     /locus_tag="PK125_00620"
     CDS             17232..17936
                     /locus_tag="PK125_00620"
                     /inference="COORDINATES: protein motif:HMM:NF012524.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HPB36642.1"
                     /translation="MRPVFIEARDLPDAWFQCIYRIFENEGIHEYVIDKGSFEGNKRR
                     EFDLVMVHIKYPGTEPLIPDIPTELGIPSPTSMEYVEEYLQYLMTDKKAENELYTYGE
                     RLTNPKVCIKGLEYSFDINPVQEVIGIYRKQKFGTNQAIMEIGMPSDITLDDPPCLRL
                     IDTRLMEDKLHFVLYFRSWDLWAGFPSNLAAIQLLKEYMCQEIGCGDGTITAVSKGLH
                     LYEYSFGLALKRLRRE"
     gene            complement(17982..18872)
                     /locus_tag="PK125_00625"
     CDS             complement(17982..18872)
                     /locus_tag="PK125_00625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB36643.1"
                     /translation="MEEKKAIVQEVINILGIAPLISSVIIENEDSPWSEKAHIKWKKD
                     HLDVKAFVWDDPTYLYGRIYRLFLYIVDVLNPIFEYKPRISPDEQQEPTVRERYNQIW
                     SIYVDSRMERLEIENFFDKTLRKNIFIDSEKNLSSTAADTIFDALWEKESYTYPEIIE
                     YAYDLKKLKLKTPIVKVDTFNKHITDLLHGSSVSRHLENIPSSEFRETVNDIISFAAY
                     HCKDAFILSKYYGICILYNRQVFLELIPTGKDSLYLTVFDGQTNTFSTLTVTKETDTS
                     SIQELIKEKYALIAKQDERL"
     gene            complement(18873..19436)
                     /locus_tag="PK125_00630"
     CDS             complement(18873..19436)
                     /locus_tag="PK125_00630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPB36644.1"
                     /translation="MALTVFVILATWSPGLWAAQERSPRKAAPAQSTGAAAAPSKSLL
                     INDANINSALKRADDMMKKGEVDGPLKILTGVYDYSKDVLLTVKFFQGHYEKIVNDPT
                     TSQSEKEEIYIKLKRMNQLLPKYNTIREVTTYNVGYLYAKKGDTEKARKFLTEVLELT
                     PFSTKKDSISMKAKTLLLDIYGLEGEF"
     gene            complement(19713..19786)
                     /locus_tag="PK125_00635"
     tRNA            complement(19713..19786)
                     /locus_tag="PK125_00635"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19750..19752),aa:Met,seq:cat)
     gene            19938..20891
                     /locus_tag="PK125_00640"
     CDS             19938..20891
                     /locus_tag="PK125_00640"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HPB36645.1"
                     /translation="MKVLVTGGCGFIASHVVDAYVNEGYEVVIVDNLSTGKMENKNKR
                     AKFYKVDICDEEVEKVFKVEKPEIVNHHAAQISVPLSVQEPLLDAEINIKGTLRLLGL
                     SKDNGIKQFIFSSTGGAIYGEATKVPTDEEYPPVPASPYAISKFSSERYIQYYHSQFG
                     LDYTILRYSNVFGPRQIPHGEAGVVAIFTEKLLAGQQPTLNHFPEERKGMVRDYCYVK
                     DVARASINASKSKKVGIFNIGTGTGTYTIDLYTEVIRALRSKGKTIPNRFDKPKRDMA
                     RPGDIKVSTLDASKAKKELKWQPNYDLHEGLVETVDWYINL"
     assembly_gap    20920..21019
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            21183..21905
                     /locus_tag="PK125_00645"
     CDS             21183..21905
                     /locus_tag="PK125_00645"
                     /inference="COORDINATES: protein motif:HMM:NF024874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HPB36646.1"
                     /translation="MKVKLLGTGTCVPSLQRLSSSYLVRTKQLNVLVDVGPSVVRRLL
                     EYGYTTRDIDVIVLTHFHVDHSADLSTFLFASNYDVVPRTKELAIIGGEGFQDFYRGL
                     LTIYPWLLPKSYELFPHELQGGVLKKSGIVITTSHMEHCKESVGVRIEEGKSVVFSGD
                     TDYTHNLVRLAEGADLLFVECSFPERKAPGHLNLAEVQKVVDQAKPRRVILSHLYPDW
                     DNYKGVLHPPLLLGEDGMEIEI"
     gene            complement(21971..23758)
                     /locus_tag="PK125_00650"
     CDS             complement(21971..23758)
                     /locus_tag="PK125_00650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB36647.1"
                     /translation="MTKFQDIKKFASEIHTEIHLVKPMLKLLGFTYESKPKFFEDHVK
                     GPDVALFYSEDFRVKSSKLWGTREYYDNTLGILVLKRYGRNLHEGITGFYLEFENRIP
                     LYQIMYLLKNSHAPWGILTNGRYWILIKKPVAFEQKLIEIDLEASLLSGDEKSARLFF
                     NTFSHMGLNKTLPDILEHQRQILIKHLKDKKKVSRKVVGSFKKRVDIYPRIRDVYREL
                     FPDDTLPSTETYLKHYAIEVENNTYQKTGIVNEYDRGDICSYLFTGNQTKADFDIEEI
                     IIGQNKQYTKEDLLSLRILDMTPGFGNVTTKLLEGIAYLSFVLPYHEKTSFIAEWENE
                     KSLKKYILDHNLYGIERSHICYDALINMLNHRYGATAHHYKPGNPLIGITLKDIGNLF
                     DITKQGSLFGRNPKEILDEFREMYRLYFSLSSKIKEDLKVREEIGVKLKIYVSRIKDV
                     MDATTATFFTKDIDIKKLEDLLYSLEADESTWDIFRKKGWYTEFQHLALRNGFFHMEL
                     EFPILLTNAYDFIFIQPALNYSWEDEIPVGEATKAFIKKGMPFLKQDGKFVVLLDDEE
                     EVLLSDLQKSKKYAVKAKKNLFILTKRDL"
     gene            complement(23894..24142)
                     /locus_tag="PK125_00655"
     CDS             complement(23894..24142)
                     /locus_tag="PK125_00655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB36648.1"
                     /translation="MKKILTSFAGMLISVTDLFAQQDKFDLLPAASYDRFIVYQNLVI
                     FWIGIIGLIVIIKMKLKEVERIQKMGINKEEKNIPVLD"
     gene            complement(24151..25215)
                     /locus_tag="PK125_00660"
     CDS             complement(24151..25215)
                     /locus_tag="PK125_00660"
                     /inference="COORDINATES: protein motif:HMM:NF012263.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bc complex cytochrome b subunit"
                     /protein_id="HPB36649.1"
                     /translation="MNCPRLKNWFWERYNIDEIRDLVKQKSVPKHKYEIWYYTGGITL
                     FLFIIQFITGMVLSFYYVPHFEHAHKSIIEIVTKLNMGWFFRSLHHWGAQIAIVMLFI
                     HLWSTLLLKAYRKPREFVWVSGFILLGIAIFFGLSGYFLLWDERAFAAVRVATGGAGN
                     LPVVGGFIKAFLRGDIDVTGDTLTRFYAFHVGILPLVTFCLMVLHILLVQYHGMSVPV
                     SIKKEKLKETPFFPNFVYKDLIIWLVMLGIVVTLATLVPPEIGKKADPLAPAPENIKP
                     EWYFLFLFQTLKLFPGEILGLNGETIAILLISAGILFFFLIPFFDRKSQREERSTLFT
                     WIGLIYFLYFVLMTIIGFIS"
     gene            complement(25196..25666)
                     /locus_tag="PK125_00665"
     CDS             complement(25196..25666)
                     /locus_tag="PK125_00665"
                     /inference="COORDINATES: protein motif:HMM:NF012575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinol-cytochrome c reductase iron-sulfur
                     subunit"
                     /protein_id="HPB36650.1"
                     /translation="MKIDFSLVTRRTFLNTIFGGWLAAFMGGSLYGLIKFAFPTLGKE
                     PDFVVLKPEDFLDIPANSVKPFAWGGKLGLFFKRSDGKTVALKGICTHMECNITYLPQ
                     DRKFYCACHKGWFDEDGKNIEGPPPKPLEFFDITIEGDKLIVAKKGIKVELPKA"
     gene            complement(25656..26396)
                     /locus_tag="PK125_00670"
     CDS             complement(25656..26396)
                     /locus_tag="PK125_00670"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: protein motif:HMM:NF004616.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008177 - succinate dehydrogenase
                     (ubiquinone) activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit"
                     /protein_id="HPB36651.1"
                     /translation="MTDKDYFTFKILRYDAAEPDIEPHFQTYKIRVIPGLTILMVLLR
                     IRDEIDGTLAFRSSCRSAVCGSCAMVINGKIDLACRTQAASFGSNTIILEPLPNMQII
                     KDLVVDMTPFWDMYEKVKPYMIRKGDYPDKEIAQTEEDRKKIDQYVNCILCACCYGAC
                     PVLARNPDYMGPAAFAKLERFMLDSRDERPDNFLQNVDSEDGVWGCDTVLRCIEACPK
                     DVRPTDSIVGLRKQLVKNKLFGKGKNED"
     gene            complement(26393..28096)
                     /locus_tag="PK125_00675"
     CDS             complement(26393..28096)
                     /locus_tag="PK125_00675"
                     /inference="COORDINATES: protein motif:HMM:TIGR01812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HPB36652.1"
                     /translation="MLYHDVLIVGGGLSGLRAAVGLCEKYDVGLVSKVHPIRSHSIAA
                     QGGINAALANNPDARDDTPEKHTFDTVKGGDYLGDQDAVEIMCKEAPVIVYEMEHWGC
                     PFSRFPDNTIAQRPFGGAGYPRTCFSADITGHVLLNTLYERSVYKDVKVYSEHVVLAL
                     VTDGNLCHGAIVLDQIKGEIIPVAAKATVFATGGYGRVYLYSTNALSNTGSGIGIPFR
                     AGVPVKDMEFVQFHPTSLIGTNILMTEACRGEGGYLLNNKGERFMERYAAKAMELAPR
                     DIVSRSIQTEIEEGRGFEHELGKYVQLDLRHLGAEKIMERLPGIRRICLDFIGIDPIY
                     EPIPIMPSQHYSMGGIDTNEKSETVVKGFYAAGECACVSVHGANRLGGNSLLDTIVFG
                     KLASISVDQYLQSDGFKPDEKLLMSKKAEVEHKIKQLNSGGSERPFKILDELRLTMSK
                     LAGIFRTKDELQQALSKIIELKERYKKVNVSSSKIYMNYELIGAWELESMLDVGHIIV
                     LGALLREESRGAHSRRDFPMRRDAEWLKHTVATIGKDGEPNISYKGVTITKYEPMERK
                     Y"
     gene            28571..29083
                     /locus_tag="PK125_00680"
     CDS             28571..29083
                     /locus_tag="PK125_00680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB36653.1"
                     /translation="MKKLCILIFLIIAFLTLAIPSKGADNNKEVDSMLVSAESFFKMM
                     KERNYARIWLLLSHASQNSIIDDTYKNIMYHFKAKGKEVTYSKGEIRNNFTTGGTIAQ
                     AYWDSYLTVFNPDMVLEYSKWEIGKVGKERAQINIIYRKAERPAVIQMYKEDNVWRVG
                     LTETFRSPKR"
     gene            29126..29629
                     /locus_tag="PK125_00685"
     CDS             29126..29629
                     /locus_tag="PK125_00685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB36654.1"
                     /translation="MGDYDDRPDWRELDRRRDKSKLYGRIEEKGQKKEKPKDRYQAGR
                     VKEALDRLFMGKKGTIEHDKLYNKIHSTYGSNRFVSTVKKYIAEYGMPDNVSALLLIL
                     DAKEQDIVCSGIGKLKDLYGTLTSRQQEDIRRKLSILAMTDKLKEIRNMAAEALEYVK
                     SEVKKNK"
     gene            complement(29942..31225)
                     /gene="eno"
                     /locus_tag="PK125_00690"
     CDS             complement(29942..31225)
                     /gene="eno"
                     /locus_tag="PK125_00690"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011001079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HPB36655.1"
                     /translation="MSSIYDIFAREVLDSRGNPTVEVEVTLESGSMGRALVPSGASTG
                     EREALELRDGDKKRYKGKGVLKAVENVNETIAPKLEGLDALEQASIDNLMIELDGTEN
                     KSNLGANAILGVSMAVARAAADFVGLPLYQYIGGIRARELPVPMMNVINGGAHAQNSL
                     DCQEFMIVPVGTDCFRESIRMGVEVFHTLKDVLKDKGHSTGVGDEGGFAPQIKNTKEA
                     LDTLMKAIEKAGFKPGEDILLALDVAASELYQAGGKYKIDGNTWTSDKLVDFYDKIIN
                     DYPIISIEDGFAQNDWKGWKKFTDKCSSRIQIVGDDVFVTNPNIFREGISKGVANAIL
                     IKLNQIGTVTETLTTIEIAKRAGYTCVISHRSGETEDTFIADLAVATNVGQIKTGSAS
                     RSERIAKYNQLMRIEEELGDLAIYGGVEVFYSIKK"
     gene            31529..32566
                     /gene="hydE"
                     /locus_tag="PK125_00695"
     CDS             31529..32566
                     /gene="hydE"
                     /locus_tag="PK125_00695"
                     /inference="COORDINATES: protein motif:HMM:TIGR03956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster radical SAM
                     maturase HydE"
                     /protein_id="HPB36656.1"
                     /translation="MEYTRLFALKLLQSEGRALERLYSNADMVRRKKMGDDVFIRGIV
                     EFSNVCSNNCLYCGIRASNRNVRRYSMTTEEIMEAAIAMEENNFSTIVLQSGEIRDIK
                     DDEIGEIIRKIKKKTSLAVTVSVGNRRKETYSFWRDCGMDRYFLRFETSDAELFNRLH
                     PGCTLKERLTCLDDLHDLEIQTGSGFMIGLPGEKLETLADNILLCRKFDFDMIGIGPF
                     IPHPETPLGNSPNAYSDNPELFFKALAVLRLFNPEAHIPATTAFDAVFPGEGRTLALQ
                     RGANIFMPNNTPVKYREDYLLYPGKPGVDEDPEQCLSSAVVRIKSLGRPLGKGPGHSI
                     KKRHREKRSKD"
     gene            complement(32649..33095)
                     /locus_tag="PK125_00700"
     CDS             complement(32649..33095)
                     /locus_tag="PK125_00700"
                     /inference="COORDINATES: protein motif:HMM:NF012780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HPB36657.1"
                     /translation="MQTVKDLLRERKKAVISVLPKSTVFEALKIMGEKEIGSVMVVDE
                     NEKVVGILSERDYARKVILRGKTSRETLVEEIMTPFEKMYTVKPETSVEDCMVLITGK
                     RIRHLPVFEGDKFVGLVSIGDVVKSMLIARDTMIEHLSDYISGKYI"
     gene            complement(33141..34556)
                     /gene="hydG"
                     /locus_tag="PK125_00705"
     CDS             complement(33141..34556)
                     /gene="hydG"
                     /locus_tag="PK125_00705"
                     /inference="COORDINATES: protein motif:HMM:TIGR03955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster radical SAM
                     maturase HydG"
                     /protein_id="HPB36658.1"
                     /translation="MKRQDKKYKADFIDEKYIFNLLERAKSAPQQEARQIIQKGRDAK
                     GLTPYETAVLLQCDEAEIKSLLFEAAHEVKQKIYGKRLVFFAPLYISNYCINNCTYCG
                     YRKSNTIVRRRLDFNEIENEIIALETMGHKRLALETGEDPVNCPIEYVLNSIKKIYEV
                     KDKTGSIRRVNVNIAATTVEDYQKLKEVGIGTYILFQETYHRETYKKMHPSGPKRDYD
                     WHATAMDRAMEGGIDDVGFGVLFGLYDYKFEVLALLLHSLHLEERFGVGCHTISVPRI
                     RPAEGITLEKFPYLVSDEDFKKIVSIIRLAVPYTGIILSTREKPEFRDQVIELGISQL
                     SAGSCTGVGGYHTDIEKKPGETVGQFQVEDHRTTDEVLRSVCESGYIPSFCTACYRRG
                     RTGDRFMPLAKSGEIQNLCQPNAILTFKEFLLDYASAETREIGEKTIEKHLEQIPNTQ
                     IRDETIKRIKKLEQGERDLYL"
     gene            complement(34617..35858)
                     /gene="hydF"
                     /locus_tag="PK125_00710"
     CDS             complement(34617..35858)
                     /gene="hydF"
                     /locus_tag="PK125_00710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008810228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster maturation GTPase
                     HydF"
                     /protein_id="HPB36659.1"
                     /translation="MEDTTLNQTPRGSRLHIAIFGRRNAGKSSIINALTNQNIAIVSD
                     VPGTTTDPVYKSMEILPVGPVVIIDTAGIDDTGELGLLRIEKAYNVLAKTDILLLVID
                     PSKGVDSHELEVIQKARDQKVAIIGVLNKIDIFPDASPEEISSILNIPVLPVSAVTRT
                     GMDELKLEMIKVAPKDWMSSTIIGDLISPGDTVILVVPIDLAAPKGRLILPQVQTIRD
                     ILDHDAIALIVKEQELKAAIENLKNKPRLVVTDSQAFLKVAADTPPDVPMTSFSILFA
                     RNKGDLISLVEGAKVVEDLVPGDKVLIAEACTHHRVEDDIGTVKIPRWLQQIVGGPLN
                     FKWASGHELPADIEDYKLIIHCGACMINRKEMLHRLMMAKQAGVPMVNYGVIIAYVLG
                     ILKRALAPFPQALTVLENKKT"
     gene            complement(36156..37877)
                     /locus_tag="PK125_00715"
     CDS             complement(36156..37877)
                     /locus_tag="PK125_00715"
                     /inference="COORDINATES: protein motif:HMM:NF040763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent [FeFe] hydrogenase, group A6"
                     /protein_id="HPB36660.1"
                     /translation="MMTLTIDGRKVEVEEGSTILEAALKANIHIPTLCYLPEVQAIGA
                     CRVCLVEVEGNKALQASCMAPATNGMIVNTNTERVRKARRFSVEMLLSNHPFECLTCA
                     KNLNCELQNLASELQIREVRFTGEKSAQRIDDSSPSIIRDQSKCILCRRCVTVCQEIQ
                     SVTALYTVGRGFEARVEPAFGKDINEVACTNCGQCSMVCPVGAITEKEYIDEIFEAIN
                     DPTKFVIAQDAPAVRAALGEEFGYPPGTLITGKMLAAVRMLGFDRVFDTNFAADLTIL
                     EEGSELLKRIREGGTLPLITSCSPGWIKFIEHFYPELLPHLSTCKSPHQMLGALSKTY
                     FAKKAKVNPKDMVVVSIMPCTAKKFECNRPEMSNSGYKDVDYVLTTRELAKMIKQAGI
                     DFVNLPDGYYDDPMGEYTGAATIFGATGGVMEAALRTAYEVATGKTLENLEFTAVRGL
                     QGLKEAVVPVEGIGDLRVAVAHGLGNARKILEKVKEDSTQYHFIEVMACPGGCVGGGG
                     QPFPVNDEIRSLRAQALYQEDSALKYRKSHENPAIKKIYKQFLKEPLGKKSHKLLHTH
                     YTPRSRF"
     gene            complement(37909..40962)
                     /locus_tag="PK125_00720"
     CDS             complement(37909..40962)
                     /locus_tag="PK125_00720"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HPB36661.1"
                     /translation="MESSRAHVLVCAGAACVSSGCREIRDAVVNKIVEQGLQDEIKVI
                     ETGCIGSCDLGPLALIYPEGVLYQKLTPQDAEEIVVEHLLKGRVVERLLYKEPVTAKT
                     IPSLKEIDFFKNQVKIVLRNCGVINPQSIDEYIARDGYFALAKALTSMTREEVIDEVL
                     KSGLRGRGGGGFPTGMKWKFAYQSPGDVKYVVCNADEGDPGAFMDRSVLEGDPHSVIE
                     AMAIAAYAVGAKQGYVYIRAEYPLAIERLVWAIGQAREYKFLGKKILDTDFDFDLDIR
                     IGAGAFVCGEETALMFSIEGKRGEPRPRPPFPVVKGLFNVPTLLNNVETYANIPSIIM
                     NGAAWYSQYGTEKSKGTKVFALAGAVNNTGLVEVPMGTPLGDIIFNVGGGIKNKKKYK
                     AAQLGGPSGGCIPVEHLNVPVDYESITELGAIVGSGGLIVADEDTCMVDFARFFVDFI
                     QEESCGKCPPCRIGTKRMLEILKRISHGEGKLEDIDNLIELATRIKDAALCGLGQTAP
                     NPVLSTLRFFKDEYVEHIVDKHCSAGVCSSLFDAPCQNACPTDQNAWGYVTLISEGKF
                     KEAIALIKEANPFPAVLGRVCYHPCESKCRRAQIDQPVAICALKRFAADVDMATFPSF
                     RPVTAKSNDIKVAIIGAGPAGLSAAYFLALKGYKPTVFEALSVPGGMLAVGIPDYRLP
                     RDILNAEIKSITDIGVEIKYNTQFGKDITSESLFKDGYGAILLATGAHKGQKLGIPGE
                     EASGVADGVTFLRAVNLNEIVKTEGKVVVIGGGNVAIDAARAALRLGAEEVSVLYRRE
                     RDDMPAYAEEIDEAEHEGVKIHTLVAPDKIISKNGKVTGVECTRMALGKFDKSGRRSP
                     HVIEGSGFTIDADMVIAAIGQLPDLSYLNGEGIKVTNNNTVEVDKYTLSAGKEGFYAA
                     GDNVRGPASVVEAVADGKKAAMAIDVHFGGDGLAPNAFRDELITMVVSYDEAEYQKER
                     KRMEMSHLPLAKRFRNFNEVALGYQANAAVEEAKRCLHCYLREQE"
     gene            complement(40978..41475)
                     /locus_tag="PK125_00725"
     CDS             complement(40978..41475)
                     /locus_tag="PK125_00725"
                     /inference="COORDINATES: protein motif:HMM:NF013426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HPB36662.1"
                     /translation="MATDNNQSSAEMQEAKLYPRLDEIIAEHSGRPEDLIMVLHKAQG
                     LFGYLPRKVQEKVAEGLNISLSEVYGVITFYNFFSTVPQGRHSVKICLGTACYVRGSQ
                     QVLDKAQKDLGIKVGGTTEDRRYSLDVVRCIGACGLAPAMLIDDDVYGRVKSTNLANI
                     LEKYE"
     gene            complement(41512..43266)
                     /locus_tag="PK125_00730"
     CDS             complement(41512..43266)
                     /locus_tag="PK125_00730"
                     /inference="COORDINATES: protein motif:HMM:NF040763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent [FeFe] hydrogenase, group A6"
                     /protein_id="HPB36663.1"
                     /translation="MIKATINNVKIEVPEGTSILWAAKKANVNIPTLCFHPDLKPGGQ
                     CGVCVVEIEGIPGLKRACMTPIGEGFRVYTHTQKVMSARKEIVEMILANHDSDYLTCI
                     KNNNCELQTLAATLGIKIVEYAKLEEKLPIDHSSISIVRDPNKCILCGRCIQVCSDVQ
                     SVNALAVNHRGADTVIGTAFSAGLGNSVCINCGQCVVHCPVGALYENSSVEAVWEALH
                     DPDKFVVVQEAPAVRAMLGEEFGMPPGSLVTGKMYAALRRLGFDAVMDTNFSADLTII
                     EEGTELVSRLKSGKNLPLITSCSPGWIKFMETFFHDMIPHMSTAKSPQQMFGALAKTY
                     YAQVKNIDPAKIVSVSVMPCTAKKFECQRPEMKASGYQDVDYVLTTREIGRMIREAGI
                     DLKEMPEELADDIMGEYTGAGTIFGATGGVMEAAIRSAYFLVTGRELENLDIKPVRGL
                     KGIKEAEIDIDGTKVKVAVAHSGAQARELLEKVQNQMATDGKCEYQFIEVMACPGGCV
                     GGGGQSWGSNMAKRARRGEALYAEDKSMAHRRSHENRSVQAVYEKFLEKPNSKKAHEL
                     LHTHYMKRSLVDGKVINE"
     gene            complement(43308..44888)
                     /gene="nuoF"
                     /locus_tag="PK125_00735"
     CDS             complement(43308..44888)
                     /gene="nuoF"
                     /locus_tag="PK125_00735"
                     /inference="COORDINATES: protein motif:HMM:NF010120.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HPB36664.1"
                     /translation="MSLVRNHVLISIDAGTVLAGARAVEKTLIEEIAKQGLSDEIAVL
                     ETGSIGTINQGVVLVVYPEGVYYAHVTPQDVPELVEEHLLKGRPLKRLILSEAPKQTV
                     IKKGKAGLLKEQPRIVLRNCGVIDPEKIEEYIAEGGYEAIGKALTAMKPEDIIDEIKK
                     SGLAGRGGASFSTGMKWDFARRAQGDIKYIICNADEGEPGTFKDRLILEGDPHKLIEG
                     MMLAGYAVGASQGFVYIRGEYTLSIERLEKAIAQAREYHLLGNNILDSTFSFDISVMK
                     GAGAYVCGEETALIESLEGKRGHPRNKPPYPVTEGLWGKPTVVNNVETLSNVPEIIKN
                     GAAWFRGYGTEKCPGTKVYTIIGNVATPGLIEAEMGTTLRDIIFEYAGGIKAGKKFKG
                     ALIGGAAGAFLGPDMLDVQMDFVNLREYAAVLGSGAILVMDETADIVDMLQSVLHFFR
                     HESCGHCVPCRLGTAQLAKIIDRIAASKGIQGDVDKLLKISDTMRDTSFCPLGQSLHL
                     PISSSLKYFRDEIDARIH"
     gene            complement(44885..45343)
                     /locus_tag="PK125_00740"
     CDS             complement(44885..45343)
                     /locus_tag="PK125_00740"
                     /inference="COORDINATES: protein motif:HMM:NF013426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HPB36665.1"
                     /translation="MYREAILKKFKPSLDNILYILHDLQDNNPQRYLAKEDIRVCADY
                     LKVPYSYVHSVASFYTMFSLEPRGSNIIRLCDSPPCHLMGSKSLLDYLKKALKVDVGG
                     TTKDGKFTLELTSCLGVCGVAPAMMINDEMVGNLTPEKVDAILEKRRQES"
     gene            complement(45359..45718)
                     /locus_tag="PK125_00745"
     CDS             complement(45359..45718)
                     /locus_tag="PK125_00745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB36666.1"
                     /translation="MNLEELKKIREKAQKDVELRQKQARIRIVVGMGTSGIAAGARDV
                     LKTLLEEIGRRNLTDVIVTQTGEKGLASQEPLVEVYEEGKPLVVYGNMNQERAKRVVV
                     EHVVNGNPVTEFALEVM"
     gene            complement(45759..46289)
                     /locus_tag="PK125_00750"
     CDS             complement(45759..46289)
                     /locus_tag="PK125_00750"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPB36667.1"
                     /translation="MMDEIASHIIDIVANSVSAKATHISVDITQDTEKNLLTLRIKDN
                     GVGMDETTKQKVLDPFYTTKKGKKVGLGIPLLKGTAETTNGTFCLSSIMGQGTEIFAS
                     FDLSHPDLPPLGNIRDTILVLVVGNPDTDFCFCIKADAKEFTLDTKEIKNLLDGVPIN
                     HPEVITFLTKCIDENM"
     gene            complement(46563..47279)
                     /locus_tag="PK125_00755"
     CDS             complement(46563..47279)
                     /locus_tag="PK125_00755"
                     /inference="COORDINATES: protein motif:HMM:NF014826.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HPB36668.1"
                     /translation="MSTYSCDLHIHSTLSPCGSLEMSPNNIIAKAKEVGLHLISVTDH
                     NMVENSLPTYTIGKKFGITVLFGIEVQTSEEIHLLAFFDNYDLAHTFQDKIYNLLPDI
                     QNDAEYFGDQVVVDEENEIIRFETRLLLNSAQISITDATKWIKDHGGLAIPSHIDSPT
                     FSIISQLGYIPDDLPFDALEVRNKEKIIDLLPLIMKKDIPFVTFSDAHYLKDIGRRRI
                     SLDLKKPNCSEIANALKQLL"
     gene            complement(47285..>47634)
                     /locus_tag="PK125_00760"
     CDS             complement(47285..>47634)
                     /locus_tag="PK125_00760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB36669.1"
                     /translation="GGKMKLSDLAKKTGCEILTGNVNLDKDIEDGYVSDLLSDVIGNI
                     KENSVWITIQRHMNILGVAKLKDVAAILIPRNLQLEDSMIEKASEERIAILRNPRSAF
                     ELAGLIYNLLQKG"
BASE COUNT        12756 a        10484 c        10390 g        13904 t
ORIGIN      
        1 taaggtggat atgatcgtgt ggtataaaat gtggaaatgc tggttcaaac atcttaaaag
       61 gtttcaaagc tccgacaatc gataacacag gaaagaaatg atgaaatgct ttaggaaggc
      121 aaggtttctt aaaaaagacg gagactttat acgataagtt gcaggttgtc catgagtcta
      181 ctcttccgat tatgggggat gaacagacag aaaaaatgag taatggcgct cacaaaagac
      241 ttttttaaat gacagcaaac aaccatatgg ggacagttta tttttttgtg attatattga
      301 ttggtaaatc ggaggtaatg ctatgaaaaa tatcttgctt ttaatcgtga ttgttgctgc
      361 ctggtttgtt ctgcagaaat atatccttcc acggtttggg atatctacct gactatccaa
      421 cagctgccgc cccggggacc ggggtattga aaaagatgag aaaaaggata gtaacgattg
      481 atcaaaatcg tttcaacttc ctcacatatt atgctttcat tcagtaatat cagcaaaaaa
      541 cacgtaacac tattattcat gctgaattat cagtgacagt aatgaatatt ttttgattga
      601 taatctctat atgcacaggt atatcatatg agaaaaaacc attaatatgg tgacacataa
      661 caactgctat gactggaata ggtccacgtc taaactatcc tcaccttggg gactggatag
      721 tctggcgtgt ccaccgtaaa ttcagtgatg tgtaaccgag ggaaaggaag ggattgatga
      781 gacaggaagc ggttatgatg cgctgggtga ttctcgcgca gctatgcctt tgtatgttgt
      841 catatgcact gattttccaa tcgatcccgc ccattatgag cttggtgatt tcccaattca
      901 aactttccca ttaccaggct ggtcttctca tgagtctttt cgcccttccc ggcattttgg
      961 tttcgttgcc tgcaggcatg gttgccgatc gttatggcgt caaaccggtc ggtatctttt
     1021 catttttgct tgcactatta gggagctttc ttgttgcgaa cggcccatca ttcgagatgc
     1081 ttctagtggg gagaatcctt gcaggaatag gtgcatcgag tctggtaatc accattcccc
     1141 aggctatcgc ccaatggttc tcggaccgtc atatgggtat tgccatgggg atctacaata
     1201 ccgcaatgcc aatcggcact atcatctcct taaacggcct ctcccatatg gcaggagttt
     1261 ggggctggag agctgctgtc tgggccacag tcttctttac tgccattatc tttgtcatat
     1321 ttgcggtttt ttatcgacaa ccagatcata aagatagatt ggaaaaccag ggaaagaagg
     1381 atcagaaccg tgaaggaatt ggcacttcca tattttttgt tggtttttca tgggccttgt
     1441 tcaatgcatc cattatctct cttttcacct ttgcccctga ttttcttgtc agcaggggaa
     1501 tgacgctcga tcatgctggt ttttccacca gcctggtaat gatcgcttcc atgtgtctga
     1561 gccctatcgt aggccttatt attgataaaa ttggtcagaa agagttgttt atcgcagcgg
     1621 gcggactggg aatggctgtt ttcattgccc tgattcctgg tattggagga gctttcagcc
     1681 tttttctgct ctgtgtcggt gttgcaagcg ctctcattcc tgtatctgtt ttctctcttg
     1741 cagccgatgt ggtaagcttc cagcgcctcg gtttaggcta cggaattctg tccatgctca
     1801 acaatgtagg aatgtttgtt ggcccgcagg ctgcgggtct gggccgagat ttgaccgggt
     1861 cttatcagat aagcttcctt gtaatggcgt tcttcgcgtt tttctgcaca ataacgattc
     1921 tgagtctttg gctaaagagg cgtggcgcat ccagtgggaa tcgctgattg ccggtaagca
     1981 gcacccttac ggaagaatat attggttttg aagagatcgt caatggtata catgtattct
     2041 tctgctgact gcttgcagag ggctttatcg aaaatatttt gaaaattatg ctttcaggag
     2101 ataagatgat cgttgagtat tcctttggca aaactcgggc atttttgggc gtgagatttg
     2161 tatgcagcta tcgcacgggc cagggggaga tgagtgccac aatcccctgt ttactggtca
     2221 tctggttgga ctatcttaaa tgtctgttca cataattcca gtcaacaggc tcagccctga
     2281 agccctgcgc ggcgttattc aggaatttgt ttcaagggaa ggcactgatt atggtgaaag
     2341 agaagtatct ccagaagcaa aattcgaaca ggtcaggtat aagcttgagc atggactggc
     2401 agttttgatt tttgatgacg agacccaaac aaccaatatt ctttcagcag atgaccctat
     2461 cttgagaagg ataaatggat ttgctgagtg accactgttc aataggtacg gcaaatacat
     2521 gggtttgtca attattggca ggtaaggaaa ccgtgcaaaa taccagtcca gattataact
     2581 tcctgaaatt gaacatataa cctgtgggta acatacgcaa acaaagaacc aaaagtaagc
     2641 atacttgttt tcaatttgta attacgaaga tgtgaaaaag gggtgttgcc caaaaccgat
     2701 cactgagaca aaaaaccgtg acccacatta gctctcctcc cataaataag gtttgataaa
     2761 gcttacttta catcaattgc agagtatagt atcaattatg aatatttctg ggagagttta
     2821 aaagacaaac atttaagata agatagagag aaaaatcaaa gatgacaaag gaccttatca
     2881 tacagtgaat ggtgatcgtt caaaaaaaca agaggtgaag aaatggtagg gccagaacaa
     2941 atcgttccgg aagaaataag agacagaaaa aaggcactgg attttgtcaa tatcgtaaaa
     3001 gccttaaggg tattggtgtc aaaagaggga ttcccctatg acagaattaa caagctcctt
     3061 gggatggttc aaagacacgg agattgttct tgtgaaaact gtcaggaagt aagaaaaaag
     3121 gagactctga gatttaatcg gtctttatca aaaattgcgc ggtttagacg agagtattat
     3181 gatgcaggac gggatggtga gtttgaggaa atcattaaaa ttgccagaaa aggagcagaa
     3241 cttctctttc tcctccagcg tgccggtctg gatgatgttg agttgataag agcaacaata
     3301 aagatatacc gaaaaaaaat aatatttgtt tgaattattg tggtgaagtt tttttgtagt
     3361 agtagtggag taaagcaaaa ccgggatagt gtgagtttgc aggtgcgtgc taccatattt
     3421 gctatcaata ataatgcaaa attgtcagag acaggtttat gtgtgtgttg aatcatgata
     3481 ccaaaaataa tgagatacgg ttgttccata tttgtgcacc cctgatgatt acgaagttgt
     3541 tatcaccata ataggggtaa tgggaataaa aaagcgtgtg aaagtatcga aagcagaata
     3601 acttattgaa tttatggaat tcacatggcg gagaggcagg gatttgaacc ctgggtcccg
     3661 tttatagcgg aacactcgct tagcaggcga gcaccttcgg ccgactcggt cacctctcca
     3721 tggcagaagt atactgatta aactgcgaaa aatcaatcca aactgcttca ataatagaac
     3781 atgttgaaag ccttacttta tttaaagttt attatcattg aaaaaatttc gcttgacaca
     3841 tttgtcgcga gtcgctatta tcgaattaag attttcagcc cgggcgatgc acatgtaaac
     3901 tgaataatgt ctttttacta cataatagaa gagatgtgta aagaccttat gaataagaca
     3961 atgggtaata tgagaaatat tattacaggc gccataatta tttgtcttgt gagcattgtt
     4021 ggtctggtgg ggtgcgacga caagaagatg tctcccatcg ctgcaaatga agtctctgca
     4081 gaaacaaaaa tcaggcaagg tacccaagta aagggtgaca cgcaaccaat agggaatgca
     4141 cagttttttg taacatatag atacactgac ccaatgacgg gtatggaggc cttccgtctt
     4201 cttatcccaa aaggctggca ggcccaaggg cagattgcat ggtctgcaaa tcctgcattg
     4261 cctgctcagg cccgttttcg tttctggaat ccgaatggta gcggtgagtt taatctgttt
     4321 cccacccagg cgtatttctg gaccaataat cgcttgtttc tcagcaccaa cccaccaggg
     4381 tcattacgat ttaacaccct ggtaatgaga cccgttgact tgcagacagc atttacaaag
     4441 atagtcattc cttatgccag aaaaggtgtc agtggaatga aaatgataaa ggcaagcgat
     4501 gttcctgaat tagcaaatat agctaaagga caacctgtac aaggggtccg cagttttgca
     4561 aacggtggta agatgaggat agaatatcag gaaaacggca ggccgatgga agaagaaatc
     4621 tatgctgctg tcagccagtt tgttactgac ctgccaggtt cagcgcttgg tcccgggtat
     4681 tttatcaact actggtatat tgattatgtg ttttctttta aagcccggaa aaacgaactt
     4741 gattcccagt ccaaagtttt tcagaccatg gtttattctt tgagggttaa tccacggtgg
     4801 tttgccaaag tagccaacgt aaaagagatg atggttcaaa aaatcatcaa aggcattaag
     4861 gatgttggtc gcatcggaga aatggttgca cgggctggca gtgaaatgag ggaaggacag
     4921 atgaaggatt gggaacgtcg gcagcaggca aacgacagaa ttgttcagaa ttttactgat
     4981 cacattaggg gtgttgagcg gtttaatgac ccttatgctg gcaaagaagt agagttgccc
     5041 accggctatg ggagagcctt ctcaaacaac ctgggcgagt atatcgttac tgacagcccg
     5101 agctataatc cgaatatcgg ttccaacgtg acctggcagg aactaaaacc ggtgaaatag
     5161 gcaaagataa aatctatttt atattgatac tatgattttc gtgtgacatt tatgctctat
     5221 tatgaaaaca aaactatttc tcattacaat acttgtttct atcgcagtaa gcacagttat
     5281 gtttcttggt ttgacggcat cattgacagc tcccagttcg agtatgaaga ctcaggaagg
     5341 cataggttcc atacttgaca tcccagccaa ctggagcatg ggcacatttc catccggcat
     5401 gagttacttc gcagcggcgg atggtaaacg tgtgactagg gatgctgatg gcacgaatgt
     5461 gtctgtcctt cttggcttag cagccggtta tcggaaaacg aatttccaaa acgccaggga
     5521 tgctgcagac cagacaatcc gcttctatca aagcgataat cctgggttac gtgtcatcct
     5581 acgagaggcg gcaaaacttg gtggttttcc acctgaatct gtattaattg aaagctcaac
     5641 aggccgtgac ggcgagatgg aaaggagcct gatgctgatt accgcaagag aaggacaaat
     5701 gttcatggca gcgttcacaa gccctgccaa cgattacaac agcttgcaga gaaatttgtc
     5761 caaatagcag actcgatccg tcttacagcc tggcaaaagg gaaagacaac accgggtagg
     5821 ccagggacgt caacatcgaa gaaacgttaa tacttgatcc gtatccacat gatttttacc
     5881 gaacgaggaa cgtaacggaa tcgaggatat ggccgtcttc atcagtaatg gcgagtttgt
     5941 ggacgcccag ttttggtgtc cataggtaga cggagtcatt ggtcccgatt ttctctttgt
     6001 ttaatgtcca cgtgaagcga cgagattgtg gttcgaatct gaagggaaca cgttgaagat
     6061 gttgaggtat ctcagggtca atggcaatca gcgtttcctg ggtagggtag gtgattcgtg
     6121 gcttcgcgtg aactctgttg accaatacca tgccggcagg ttcggtacct gctataaacc
     6181 attctgttcg tggcggttca atagaatctt caaaagtgag ttgggccgat tgtactcccc
     6241 ctgggggttg aggtggtttt gaggcaaaac cgcgatgaag ataattcatg atgcccaacc
     6301 aaacaggtgc ggctcctgtg atgcctgtga cgttcctcat aggctctcct gagaagttac
     6361 caatccagac accgactgtg tacgtgtcag aataaccaac acaccagttg tcgcgcatat
     6421 ccttgctggt ccctgtttta acggctgtcc agaaacgtgt tgacaggtgg ttttcaaggc
     6481 cgaaggtggt gcttcgagcc tgtctgtctg aaaggatgtg ggagataaga aaagttgctt
     6541 ttttgtcaag aatattttgt gcgtgatgcc ggttcccatc aaataccagc ctcatattgc
     6601 tgaatcttcc attattggcg agggtccgat aagcattgac aagttcatag agggttatgt
     6661 ctgctgagcc cagggctatc gagtatccgt agaattcggg tgcctgagaa atgcccttaa
     6721 aaccaagttc ctgcaagcgt tccacaaaat ctgtaacgcc gacgaggcca aggactctca
     6781 cggctgggat attcattgat gaagaaagag ctgtgcgaac acttacaggc cctctgaaga
     6841 tgttgtcgta attgtggggc acgtaaagac ctgtaggggt gactatttcg aggggagaat
     6901 cttcaagaat tgaagacggt gtaagtagtt ttttctcgag ggcaagttca tacagaaagg
     6961 gttttaaagt tgaccctgcc tggcggtacg ctgtaatacc gtctacatac agggttgtag
     7021 gggatgttcc gctattgccg acatatgcaa gtatgtcccc tgttttattt tctaccacca
     7081 atgctgttcc atcagaaaca ttttgatttt caagaaaacg cacagcctcg ttcaaggaag
     7141 cgtgcacgta tcgttgcagg ttgccgtcca gggtgcattt gctctccttt ttcccgttcg
     7201 tcaggagtaa tctggctaca tggggtgcaa tagaggcatc cctgcgtatg tggtaggtcc
     7261 gaccaagggt tttatacgcc atgtcccgga tactgttgcg ggaaacgggc gaggcaaagc
     7321 ggcatgcacg ctttgcaact tcctctactg aagcattggg ggatgtgatg agtgaggcca
     7381 gaataagtga ttctgcctct tccaaaccac tcggttcttt gttgaaaagg cctcgcgagg
     7441 cagcacagat tcctgagagt tcaccgcggt aggtgatgag gttcagatac gcttcaagga
     7501 tttgatcctt ggtccagtga ttgtctaaag acacagcagc ctttaactgg ttccattttt
     7561 gcaaaatccc tcttctgctt ccattgcggg gcctgagctt cctgtcaagg atggaggtga
     7621 gctgcatggt gattgtgctg gctcctcgca agttcttatg tgtcaggttg ctgaatgtag
     7681 ctgaaacaag ggcaagccag tctattccgc cgtgactgta aaaccttttg tcctcaacac
     7741 gtaaagtggc ccgaaccatg gagggagata cctgaccgag atttgtccag tcgagtcgcc
     7801 tcccatggac atctacccgc agggtgtgga tcacttctcc attacggtcc agaagtgcag
     7861 cgtctgatac cgcgtacttt tgttttatct cttcaaagct tgcacatggt ctgccgttgg
     7921 cgcttctgta aatgtcgatg agaacccaca gcaggcccgg tgcaataata aggacgaggg
     7981 cagccaggac aatcctcatc ttaatagtag attttcttct tatacttctg ttattgtggg
     8041 tgaaccacca tttttgcatt tggaagctct ccaaacattt caggctgata gagcgcctct
     8101 accctcgtct gggggagatt gaacgcgcct tcattgttga gtcgcattgt atattctatg
     8161 gtaaactttc ctctggcaat atactcgaaa taggctctca tggattcgaa cgatcgttca
     8221 cggaaaacct cctgtaccag tcctttccct gtttcttgtt ttgtcaatat ggatgaatct
     8281 cttccgagac cacctcccag tatggttgaa cccgctggga tcggatcgtt caccacgacc
     8341 catgtcatgt cgctttgtga atcaatctcc aggttcaccc gtaccacgtc tccccttgtc
     8401 cacttcccgg ctgctttctg ttcaatgggg gtaatagttt tttttatctt aaaacctgag
     8461 gcaagagatt ctttcaatgg aatcgcagct atagactgaa tggtgaccca tggttttccg
     8521 gttccctcat gtttaatgaa gatttcttca ttcttttggg gccaggaaaa gaatgactca
     8581 ttgccattcg gggtcttgac ccaatctgtt gcaacggttt tgttaacaag tgtcgaccag
     8641 gttatacccc ctacagggag agattcaaat ttctttgaga acttgtccat agcaataata
     8701 ccccaggcat tggctactgt tgtattccag cgaccatgcc tgagacggcc cacaacaccg
     8761 cgagcaatac ctggactatc tacattccaa ctatcaaact gaagggcagt aagcagggtc
     8821 ttgatagcat tggtatcggt agataccata agccaccagc aatagtctga tcgttctgtt
     8881 gagaaattca tcgccgttcc ctggaggatg agacgggacc ggagtatgga ttgcgccttt
     8941 ttcattttac ccaacctatc aggaatatcg gttaccctgg agagaatgcc gatccagtca
     9001 atgactgcgg aagtgggcca tagatccggt tcgatagtca ccgactcaag caggtttgcc
     9061 tgggcctcat cgtaccgtga aagtgcttcc aaagctgata gtttccgtat agaaagatca
     9121 gcagtaggaa ggtttgacca tctaaccaca cgcccctgta caaaaccctt aagcccttcc
     9181 aacatttttt ctttcggtat ttttggtatc tggtagccgg cctcatgggc aatagagagg
     9241 atgtaagcag tgagtgtatc agtccccgaa atgaaacgaa cagggaagta ttttatcaac
     9301 ccgtcctgat caaggtaaga ggggaggtcg ttcataaggc tgccccaaat tttcttatca
     9361 cgcagcgcga ttgccctgga taccttctgt tcaaaacatg tataggagta gtccttcatg
     9421 tattctgtca cactgcctaa cccctcagca agcttaggtt ttattgatac cctgatgccg
     9481 cccctcgaag ggacagcatc aggtggaaac tggacagcaa tcctgtgctg ctctttcagt
     9541 tgtgccaatg ttgcctggaa tgttctgagg ggtactgtag gggcgacctt ctgggtgatt
     9601 ttcacagtat cggttacatt ctctatttta tccatagccc tggcttcata gagaagtttc
     9661 tctgttcctg caggaactgt tacatcccaa ccaatctccc tcgattcacc tgcaggcaat
     9721 ttcatatcaa gcggtttgag atcttttctc tctttcaggt ctgtagtgga aagtaatgcc
     9781 cttatgtcca taagacggtc cgatgtgttc cttatggtaa agcgggcaaa gaagcgatcc
     9841 ccttcccgag ctaagggtgg gatgcctgaa atgaccatca ggtcttgggt agtattaacc
     9901 gtggcagagc ctgttccaaa aaaggatgca cctgctgatg ctatggcaac gatcctgaag
     9961 gatgttaggg agtcgttgag agggatcttt actgttgctt cgccgttgtc gttgagtgga
    10021 actgacgatt tccacaaaag gagtgtatca aaaagttcac gtgttgtctg cttgccgccc
    10081 ccgccgccat aggagaggga tttacggcca aaatgacgtt tcccaaccac catcatctgg
    10141 gcagtggagg tgttgatctc gtatggtctt cgtgccatca tagcttcgag taacttccag
    10201 ctctcattac ccttgagttc aaggaggcct tcgtcaactg cggcaattgt cacttcgcct
    10261 gcctctggtg gagatttccc aaaagcagtg gtgaccttga tttttacatc aacaacctgt
    10321 ctgaccgggt atgttttctt atcagtagta atcttcactt tgagttcgtg tttctgccag
    10381 cctacgcgaa gctctgtgat acccatcttg tatgcaggct taccaggatc gaacattacc
    10441 gatggttttg catctgccac tctgcccctg actataaatg ccgatatgaa tatgtttggt
    10501 gcgtaggaat tttttacagg aacctctatc acggggttgt ttcgggttaa tcgttggata
    10561 ctggtgtcaa tgacaccctc ccgctcaatg gtaatgagag ctgtggcttc cctaaaaggc
    10621 atttttacct gaaagcgtgc cttttctcct ggttatattg ttttttctca ggaacgagat
    10681 caatgcggtc atcatcgccc atctcgaacc atctgtcttc cctgccgtag atggaagtct
    10741 ctgtgtaggc tgtggagata ttgccggcac tgtcgcggac ttcagcctgg aggataattt
    10801 ctccttccac aggcggtttg ccctcacagg tgaggatgcc ctgtgcatct gtctttccct
    10861 gacaatggaa accagcttca acaagctccg ttgtgctttt ataagaatag aatccaccag
    10921 ccacacggcg ccggtgggtg aaaatgtttt tcttaagaag ggaaacaact gtttcggcgc
    10981 ccggtacggg ttttcctttt agatcaacgg ctatgacttt gtaaacaaga gggtcttcaa
    11041 tgcggcctga cttaacaata gtaatcccgg cgtgtatccg ggaagggtag agaccaatac
    11101 gggatgagac ggtttgcgtc tccccgttag ggtccttgaa ttccagttcg gaaagaatgt
    11161 cccgtggtgt atcaatttca ggaagatccg acagatttaa ccgggcagaa cctgtagcat
    11221 cgagtgtaag gtcgtgtgtc ttcaatttta tctcctgacc atcactgcga ccgtttctcc
    11281 ccggggaaac aaaagaatct tcttgaggac catcttcttc atggacgggc aaaggttttg
    11341 tcaggctttt ttttgcgtat ccgtttgaga acatatattc gctgtagtcc tcgaagttta
    11401 tgaagcgtgg ccgtatctct gatctgagct ttaccggtgc gaacgaagca cctcccccag
    11461 agagataggt gagtgaaata tccacgtcaa catctttcgc attaatcaca ggttcttttg
    11521 gacccttgac agaagctcgc atcaaaggaa tacggaactc ctgcacctca aaggagcctg
    11581 tattccattc taccttttgg cgtacacttt tatccccttt ttggagcaag aatgtccggt
    11641 aagttcccag ttttgcgttc tctgggattt tccatgttgt ttctgctgtt ccccttgctg
    11701 tccactgcaa cggaaatgag tattcctgat cagtgcccac atgctggatg atcagcatgt
    11761 cagggagctt ttttcctgtg ggaaaggtga tgccatgtgt catgtcctgc tcccggataa
    11821 aatgcttcat acttactgtt tcgccggacc gaaaaagctg tcgatcaaaa accgtatggg
    11881 taatcgtgtt tcctttatca ccattatcgt atcttgtagg cagattgaat ctccagggat
    11941 ctatacctcc ggtccagctt gaatgggtaa aggtcaaatc gttgccttta ctcgcaaaca
    12001 cgaatatgcc tgaagtgata ccgctcaaaa gctgggaatc ttcaaaattg gtccagttat
    12061 cctggttatc ttttctttcc acggggcatt tggtgagctt gtcctttgat ggcaaggccc
    12121 taccaatctt tgcaatgcca tctctatctg ttttccctcg ccagatgaca tttccagaac
    12181 aatccctcag tttcacatga gcagcgtcaa ctggttgggc cttatcaagg gaagttaccc
    12241 atactaaaga tgattccctt ccccatttga aatgggcaac catgttagaa accaatactg
    12301 acgtgggtat atggattggt tggggtttcc cgagaagccg ttcacccagg agcttgctct
    12361 caagctcaac aacataaaaa cctttatcct taagaggaat gccgatcacc tcaaactctt
    12421 ttgagggtcc aggttttgga acagtgaatg tagtctcctg ccctgtgccc ttaagtacag
    12481 actccctcct gtctgtgaag cgaagacgtt tcagccattc aattataacc tcttcgttgt
    12541 ctgccccaag gtctctgact ttccccttta cctcctgtgt caagttcaga tatttaccac
    12601 ggtctgtttt agactgatct gttgctccct tctccgggag accggtctga tatgcccatg
    12661 cctgaattgt ctcttcaata ttccttactg tcacaggcag ggatgtttca tctgtatgtt
    12721 caataatacc gaaatgccct gaaaattttg ctaatggagg atagctgtgg gtcatcacct
    12781 tgagaggaaa tttcttttcg ttcgtaagtc gtctccctgt gtcgtccttg agccccctgg
    12841 ggatgtctat tgtaaaaggc gtattttcag ggaatgggcc ttcaaaggaa atggattgga
    12901 ctgtaagatc ctttcttttt tcatctgttt caggcttttt caccgaagaa acaaggtatg
    12961 caattgcatg agaatactct atttttgatg gtttccactg ctgtcccttc tcgtttttga
    13021 gagtaatgtc cctgacaaag ttccacggaa caggagagga aaattcgagg ttcatggggg
    13081 agaggggcat acagtttgct tttggatctt ctttcatgca gcggaaggtc acagaaaacg
    13141 gagtgcgggt cttgtaggaa atcttttgat cttttgtcgt ggcaatgccg gtctttgatt
    13201 taacaccttt gccccaaatt atgtcgattc gtgcttgtga agggaactgt tgtctgcaat
    13261 caaacactgt aacaggtgca tgatccttgt catgaccaat ggatttaaga aagtcatttt
    13321 tttcagaacc ggtcaaaagt cgaatgccaa ccctttcttt gatcccttcg atggaacagt
    13381 agacgttttg tgtaagcgat gtatcgtcag gatcaccatc gaggaagaag acaaaccgtt
    13441 gctgttcatc aatgtattcg tcaccctcgt acggctgagc tgtaatgacg gatgggcccc
    13501 ctgtgttgaa tcgaaagctt gtctggccga caatagactt gcctgcaagt gacttgagcc
    13561 cttttttcaa cctgaaaaca caggaaacac cagaagggag atctctatcg aaatcatagg
    13621 accagttctt gccgtctatc catcgccctg tgcccttttc agaacagctg atatcaaagg
    13681 ggcctgtaag ttggggatca ccaaaggcca ccatttgatc ggaaaatcga acctttacct
    13741 ggtctacttt tttggcggtt ccttccggag aaaaaaactc tatggttgca gcatcagcaa
    13801 taacagtcca acaggctata gagaacaaag caaagagaaa aggccaaaat aactgacgtt
    13861 taaccattca cgtacccccg gcattatgcg tttatttttt ctttccctgc gatctgtata
    13921 ttctacccga actatcaggg cagtcaatca attttaggat aaaacagact tgttacacga
    13981 gaaagcattg tatgatataa aaaagggggt aatataacac tcatatccga ggtctaatga
    14041 tgtgcaacaa atcccaacag tcaaccctaa accctcagct tgctttactc cagttccgtc
    14101 acaaattgat caaggcattg agagactttt tctacgaaag gaattatctg gaggttgaaa
    14161 ccccgaattt aatgacgacc gttgcccctg atccgaacat tgatgccctg aaagtcttta
    14221 ttgatgacag gggaccattc ttccttcaca cctcaccgga aatggggatg aagaaaatac
    14281 ttctgcacgg gcacaagaga atatttcaga tatgcaaggt ttatagagtt gaagagcagg
    14341 cagatattca taatacggaa ttcaccatgc tcgagtggta tagggaggga acataccttg
    14401 atgcaatgaa agaagtggag gaactcatat cctgtgttgc cggcatgctt accatagaca
    14461 acagggaagt tttcgcgcgg ccctggaaga tatatgaact gtctgaactt ttcatgacaa
    14521 tgacacatat caatcccttt gggctcacac gggatgaatt gtttgcatcc atggaatcac
    14581 ttggttttcc aggcattgat gaaagagatg catggaatga cctttttttt aagctcttca
    14641 tccaagaagt tgagcccaaa atacctcaga atgttcctta ttttatcatg gactggccac
    14701 tgtccgtgtc aacgatggca aaaagaaagg atgaaaacag ggtagagcgg tttgaacttt
    14761 acataaaggg cctggaaata gcaaatggtt attccgagct ccttgatgtg tctgagcaga
    14821 ggaagcggtt tgagcaggat aatctggaaa gaaaagcatc aggaaaacag acttttgtaa
    14881 ttgatggggg gttcatagat gccttgaatg gcatcaaagg accttgtacc ggtgtctcaa
    14941 tcggggttga ccggctcatt atggttcttc tgggaaaaga gcgtattgag gatgtgctga
    15001 tcaacaggat aaggttatag gagataggag atatgcgata gacgacaggc ggtggacgat
    15061 agggataaga aaagcagctc atggcaggct ttgtttctat ttctgttttt cctgcctgtc
    15121 ctgtcgcctc tttcctgttt ctcaaaaaaa ataatcaggc atgtctgtca atcgattccc
    15181 tgtttacatc aggctttttc tgcaagctgt gaaccttcca caggatctgc tgaggcatat
    15241 cactgggggt agcacaccca tgtcacgcat gacatccaca aaggcatatc ctgtgccctt
    15301 tggtgtaaca ttaatattcc tcatggtctg tgagcgaaga agggcgatct gctggtgcgc
    15361 aaagttggtg gtgttaccca ttgggtctat cttatcaaat tccacagtgc ataggaagtc
    15421 aatggtagca atgcctttgt ttgaaagttg gacaggaaac gcaggagcta tagattagag
    15481 ttttccggag aagtgattgg gttatctggg gttcttacca aaaaggcacc tatcgtgtgc
    15541 ggactgtcaa tatactcaaa atactctggc ctgaatattt cttaagaggg tgtaccggac
    15601 ttgtaccgtg ctatcaggtc tctgattctt ggtatagcct gttttgcagc ttttattcct
    15661 tcatccatga gaaatttttt ctgggtgaag tcaaacatgc caacaccttt tgtgtttggc
    15721 tcaatcacta tgtcagcatg ctgtaatttg tttttactcg cttcatgcat catgattgct
    15781 gctgattgcc cgactatatc aatgagcgag tttatattag cttctcttat gtctctactg
    15841 atattcacgg cgattacaat atctgcaccc ttgctctttg ccacatcgca ggcaatgttg
    15901 tttgtaacgc ctccatcgac gagcaccctg ttatcaaatg tgacgggaac gaaaatcccc
    15961 ggtatagctg aggaagcccg aactgctttt gcgacagaac ctttttcgag gacaatggtt
    16021 tcacctgtgt tgagatcagt tgcaattggg tagaatggta tccttgtatc ctcaattctt
    16081 tttgttttta cagtctggtc aacgaagttt tcgagccgta ccccctgcac aggcccgaac
    16141 tttgaataaa taagtgacgg gtcgagtacg tcgttccttt caattttgaa ggctttccat
    16201 tcaagctcga agctgtcagg gttggatgca taaatggcac cgataaggct gcccacactt
    16261 gtacccacga tcatgtggat cggtattttt tcctgctcca aggcacgtat aactcccaca
    16321 tgggcaaaac ctttggcaga gcctcccccc agtacaaggg cgattttcgg atcgcccttc
    16381 ctgattatag ccggggcagt agttacagtc tggcatgaaa taaaaagaaa agccgagaca
    16441 atcgccaggg caaagaactt cttaatacac agctctttca ccaacatacg gattgtataa
    16501 cttttctctt cctattgtgc tcttgggtgt atcaccataa ttatgacctg gggcgactat
    16561 cgtatcgtca ggtagtacga agagcttgtt tcgtatagaa gaaacgagtt gttgcaatga
    16621 accgcctcca agatcagtcc tcccaacacc gccgacaaac agtgtgtctc cggtaaacac
    16681 cattccattg tgatacagac agatgcttcc cggtgagtgc ccgggggtat tgagcacctt
    16741 taatgatgtc tttccgattg tgatgaaatc tccatctctg acagtaatgt ctgctggagg
    16801 agatggtttg gcatggaaca tcagcatcat atcgggcgat tgctgaacga gggcgcctgc
    16861 ctctttctcg tggataataa tatcagcacc tgtaagttct ttcatgcgcc tgtttcccat
    16921 tatatgatct atgtgagaat gggtattgac gatgtacttt atggtgtatc ccctgctttc
    16981 tgcttctttg aatattctgt cttcttctgc tgccgggtca atgacaagag cctcattcgt
    17041 tatcttgcat gcaacgagat aggcaaaaac cgcaaaacca ccaacttgca tttgttttac
    17101 aaacataatt acctcctctt taaatgtaat ctttttggtg ccctttttca atttatttgg
    17161 taaactcatt cactataaat cgggttccgg taatggtttc agaaaatatg attcctgggc
    17221 aaggaggttg aatgagaccg gtttttattg aagctcgcga tctacctgat gcatggtttc
    17281 agtgtattta tcgaatattc gagaatgagg gtatacatga gtatgtgatc gataagggtt
    17341 cttttgaggg caacaagcgg cgggaatttg acctggtgat ggtgcatata aaatacccag
    17401 gaaccgagcc acttatcccg gacatcccaa cagaacttgg aataccttca ccgacaagta
    17461 tggaatatgt ggaagaatat ttgcaatacc ttatgactga taaaaaagct gaaaacgagc
    17521 tttataccta tggcgagagg ctcacaaacc caaaagtatg cattaagggg ctggagtact
    17581 cctttgatat caatcctgtt caggaggtca tagggatcta tcggaaacag aaatttggca
    17641 ctaatcaggc tatcatggag attggcatgc cttctgacat tacccttgat gatccaccct
    17701 gtctgaggct gatcgacaca cgcctcatgg aggataaact acactttgtg ctttacttcc
    17761 ggagctggga cttgtgggct ggatttcctt caaaccttgc agctattcaa cttctcaaag
    17821 agtatatgtg tcaggagata gggtgcgggg acggcacaat cactgcagtg agcaaaggtc
    17881 ttcatcttta cgaatatagc tttggacttg ccctgaaacg tcttcggcgc gaatagcttg
    17941 tccggttctg ccctttgccc catgctctat ggtccaccgg tttacagccg ttcatcctgt
    18001 tttgctatca aggcgtattt ttctttgata agctcttgta tcgaactcgt atcggtttcc
    18061 tttgtaacgg tcagggttga aaaggtgttt gtctgaccat caaatacggt caaatagagc
    18121 gaatctttcc ctgtaggtat caattccaga aaaacctggc gattatacaa tatgcagata
    18181 ccgtaatatt tcgatagaat gaaagcatcc ttacagtgat aggctgcaaa actgattata
    18241 tcgtttaccg tttctctgaa ttctgaagat ggaatatttt caaggtgtct gctcaccgac
    18301 gagccatgca gaagatctgt tatgtgcttg ttgaaggtgt ctactttcac aataggtgtc
    18361 tttaatttaa gttttttgag gtcatacgca tattctatga tttctggata ggtgtatgat
    18421 tctttctccc acaaggcatc gaagattgta tcagcggcag ttgatgaaag gtttttttct
    18481 gagtcgataa aaatgttttt tctcaaagtc ttgtcgaaaa agttttctat ttccagccgt
    18541 tccatacgac tatccacata gatactccat atctgattgt acctttcacg aactgttggt
    18601 tcttgttgct cgtcgggcga tattcgcggt ttgtattcaa agatagggtt gagaacgtca
    18661 actatataga ggaaaagacg ataaattcgg ccgtagagat aggttgggtc atcccagaca
    18721 aatgccttaa catccaggtg gtccttcttc catttaatgt gcgccttttc actccaggga
    18781 gaatcttcat tttcaattat aacactgcta atcagaggtg caatgcccag gatgttgatg
    18841 acttcctgga ctattgcctt tttttcttcc acttagaatt cgccttccag cccgtagata
    18901 tcaaggagaa gtgtttttgc cttcatggat atggaatctt tcttggtaga aaaaggggtc
    18961 agctcaagaa cctcagtcaa aaacttgcgt gctttctcag tatcaccttt ttttgcgtag
    19021 agataaccca cattgtatgt tgtcacttcc ctgatagtgt tatatttggg caacaactgg
    19081 ttcatccgtt tcagtttgat gtatatttct tctttttcac tttgtgaagt agtcgggtca
    19141 ttcacgattt tctcataatg gccctgaaaa aattttacag ttaataaaac gtcttttgaa
    19201 tagtcataaa cacctgtcag gattttaagg ggtccgtcga cttctccttt tttcatcatg
    19261 tcatcggctc gtttaagagc tgaattgatg ttggcatcat ttatcaacaa actttttgaa
    19321 ggagcagcgg cagcgcctgt gctttgagca ggtgcagcct ttcggggtga cctttcctgg
    19381 gcagcccaga ggccagggct ccacgtggca agaataacaa aaacagtaag tgccatgaag
    19441 acaaccaagg gcctccggat ggcaaaaaat tttttttctg tattcagtat atgttgcatg
    19501 gtatgtcttt atactccttt cgttgttttc tctttttcta tttttccggt gtggaattta
    19561 cattgaaaag aatgacaggc aatggcactt aatcgcttac caatttatat attatatttg
    19621 cctatgaaat tggcatgaat cgaaacctga atcctggaac ccgcgaacct tcatcctcca
    19681 gactgtgcaa gtaaaaccaa cagacaaaat aatggcggtg cagggagttg aaccccggac
    19741 actgcggata tgagccgcat gctctaaccg tctgagctac accgccgatg tgttttgata
    19801 taagatataa tgccgcgatt gtcaatagga atttgactga agggctgtgc attggggcag
    19861 cgcgagccca atcactttat tgtttaactg aaaacggcga ttatggtata aagagctcga
    19921 atctaaatgg aggaaatatg aaagttctgg ttactggagg gtgtggtttt attgcttctc
    19981 atgttgttga tgcctatgtc aacgaaggat atgaggttgt cattgtagat aatctgtcaa
    20041 caggcaaaat ggagaacaag aacaaaagag caaaatttta taaggtagat atttgcgatg
    20101 aggaagtgga gaaggtgttt aaggtagaaa agcctgaaat tgtgaatcac catgctgcac
    20161 agatttcagt tcctctttca gttcaggagc ccttgctcga tgctgagatc aatattaagg
    20221 gaaccctgag gctgctaggc ctttctaagg ataatggcat taagcagttt atcttttcgt
    20281 caacgggcgg agccatatat ggagaggcaa caaaagtgcc tactgatgaa gaatatccgc
    20341 ctgtaccagc gtctccctat gccatttcca aattctcaag cgaacgttac atccagtact
    20401 accacagtca atttggtctg gactatacaa tactccgtta cagtaacgtt tttggtcctc
    20461 gacagattcc tcacggtgag gctggtgtag tggctatatt taccgagaag cttcttgcag
    20521 gacagcagcc aacactgaat cattttccag aggagagaaa gggtatggtt agagactact
    20581 gctatgtaaa agatgtggcc cgggccagta tcaatgcaag caagagcaaa aaagttggca
    20641 tcttcaacat agggactgga acaggtacct acaccatcga tctgtataca gaggttatca
    20701 gggcattacg cagtaaggga aagacgattc caaataggtt tgacaaacca aaaagagata
    20761 tggcaaggcc tggggatatt aaggtaagca cgctcgatgc atcaaaagca aaaaaggaac
    20821 taaaatggca gccaaactat gatctccatg agggcctcgt tgaaacggtt gactggtata
    20881 ttaatttgtg aatattgcta aaatgggaat gatctatgan nnnnnnnnnn nnnnnnnnnn
    20941 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    21001 nnnnnnnnnn nnnnnnnnnt gtgggaggca cgaaatgaaa gttaagctac ttggcactgg
    21061 gacctgcgta ccatgtacag gggctcacgt cgccccctcc acgagcagaa gcccgtggag
    21121 ccaccccttc tcgctcgaca atctcgctaa taattaaata tctatgatgt gggaggcacg
    21181 aaatgaaagt taagctactt ggcactggga cctgcgtacc atcgttgcag agattgtcgt
    21241 cttcttattt ggtgcggacg aagcaattaa atgtcctggt cgatgtaggg ccatctgttg
    21301 tgaggaggct gcttgaatat ggttacacga cgagggacat cgatgtgata gtcctaaccc
    21361 attttcatgt ggaccattca gctgatcttt ccacttttct tttcgcatcg aattatgatg
    21421 ttgtgccaag aacaaaagaa ctagcaatca taggtggtga agggtttcaa gatttttaca
    21481 ggggtcttct taccatctat ccctggcttt tgccgaaatc gtacgaactt tttcctcatg
    21541 agttacaagg aggagtactg aaaaaaagcg gcattgttat aacaacttct catatggagc
    21601 actgcaagga gagtgtcggt gtgagaatag aagagggcaa gagtgttgtt ttctccggag
    21661 atactgatta tacccataac cttgtaaggc ttgcagaggg agcagacctt ctttttgttg
    21721 aatgctcatt ccctgagaga aaggcacctg gtcacctcaa ccttgccgag gtgcagaagg
    21781 ttgtagatca ggcaaaaccc aggagggtta tcctttcaca tctttatcct gactgggaca
    21841 actacaaggg tgtacttcac cccccacttc ttttgggcga agacggaatg gagatagaaa
    21901 tctgaaataa tgtgtttcgt tcagtatata ttttttgtgt aaagacgaga gcaccttgaa
    21961 agaaaaggcg ctataagtct ctttttgtga ggatgaagag gtttttcttt gcttttactg
    22021 catatttctt ggacttctga agatctgaga ggagtacttc ctcctcatca tcaaggagta
    22081 caacaaactt tccgtcttgc ttcaaaaaag gcattccttt cttgatgaat gccttggtcg
    22141 cctcccccac aggaatttca tcttcccaac tgtaattcaa ggcaggttga ataaagataa
    22201 aatcataagc attggtgaga aggataggga attcaagctc catatgaaaa aacccatttc
    22261 gcaaggcaag atgctggaat tctgtgtacc accccttttt tctgaaaata tcccacgttg
    22321 attcatctgc ttcgaggctg taaagcagat cctctagctt ttttatgtca atgtccttcg
    22381 taaagaatgt cgctgtggtt gcatccataa catctttgat gcgcgaaaca tagatcttga
    22441 gtttaactcc aatttcttcc cgtactttta gatcttcttt aattttgcta gagagagaaa
    22501 agtagagtcg atacatctct ctgaattcat ctaagatttc tttcggattt ctcccaaaga
    22561 gagacccctg ttttgttatg tcgaacaaat tcccaatgtc ttttaaagtt atcccgatga
    22621 gaggattccc tggcttataa tgatgtgccg ttgccccgta acgatgattc agcatgttta
    22681 taagggcatc ataacaaatg tgagatcgct caataccata aaggttatga tcaagaatat
    22741 atttcttcaa agatttttca ttctcccact cagctatgaa actggttttc tcatggtaag
    22801 gaagaacaaa agagagatat gctattcctt ccagcaactt ggttgttaca ttcccaaaac
    22861 cgggcgtcat atcaagtata cgaagcgaca aaagatcctc ttttgtgtat tgtttattct
    22921 gaccaattat gatttcttca atgtcaaagt ccgctttagt ctggttgccg gtgaacaaat
    22981 aagaacagat atcgcctcta tcgtattcat tgactatacc agttttctgg taggtgttat
    23041 tttcaacctc aatggcataa tgtttcaggt atgtttcagt tgatggtaat gtatcgtcag
    23101 gaaaaagctc tcgatacaca tctcttattc ttgggtagat atcaacgcgt ttcttgaatg
    23161 agccgactac cttacgagaa accttcttct tgtccttcaa atgttttatg agaatttgtc
    23221 gctgatgctc taaaatatct ggtagggttt tgttcaaccc catatgggaa aatgtattga
    23281 aaaagagacg ggcagatttt tcatctccag agagcaggga tgcctcaagg tcaatttcaa
    23341 taagcttttg ttcgaaggca acaggttttt tgatgagaat ccagtaacga ccgttggtga
    23401 gaatgcccca tggtgcatgt gaatttttta aaaggtacat aatctggtag aggggaatcc
    23461 tgttttcgaa ttcaagataa aacccggtga tgccctcatg cagatttctt ccgtagcgtt
    23521 taaggaccag aataccgagt gtattgtcat aatactctct tgtgccccat agttttgagc
    23581 ttttaacccg gaaatcttct gaataaaaga gagcaacgtc agggccctta acatgatctt
    23641 caaaaaattt tggttttgat tcatacgtaa aaccgagcaa tttgagcatg ggtttcacaa
    23701 ggtgaatctc tgtgtggatt tcacttgcaa atttcttgat gtcctgaaat ttagtcaaca
    23761 atccagtgag ggaagaatat acctctccat caataggcgc tgaggagagg gtttctatac
    23821 ttgaaggtgt acagagagta ttttgcaaaa tcagcggttg catggtcgta gaatgataat
    23881 cgatgccgga gatttagtca aggacaggaa tgtttttttc ttccttgttt atccccatct
    23941 tttggatgcg ttcgacctct ttcaatttca tcttaatgat gacaattagg ccgatgatac
    24001 ctatccagaa aatgacgagg ttttgataca caataaacct gtcgtaggag gctgcaggaa
    24061 gaagatcaaa cttatcctgt tgtgcgaaaa gatctgtgac gcttatgagc attccggcaa
    24121 aagatgtgag tatttttttc attaattttc ctaactaata aagcctatta tagtcataag
    24181 gacgaaataa agaaaataga tgaggcctat ccaggtaaaa agagtgcttc gttcttcccg
    24241 ctgtgatttt ctatcaaaga acgggataag gaagaaaaac aggataccgg ctgagatgag
    24301 aaggatagca attgtctctc cattcaagcc caaaatctcg cctgggaaaa gcttcagtgt
    24361 ctgaaacagg aaaagaaaat accattccgg tttaatattt tctggtgcag gtgcaagagg
    24421 gtcagctttt tttccaattt caggtggtac aagtgtggca agggtgacaa caatcccaag
    24481 cataacaagc cagataatga ggtctttata aacaaaatta ggaaagaatg gagtctcttt
    24541 caacttctct tttttaatgg atacgggcac gctcatgcca tggtattgaa caaggaggat
    24601 gtgcagcacc atgaggcaaa atgttacaag ggggagaata ccaacatgaa aggcgtaaaa
    24661 acgggttagc gtatcacctg ttacatcaat atcccctcgc aggaaggcct tgatgaatcc
    24721 tccgactaca gggagattgc ctgctcctcc tgtggcaact ctgacagcgg cgaacgccct
    24781 ttcatcccag agcagaaagt agccactgag cccgaagaaa atggcaatcc ccaggagaat
    24841 gaatccggat acccatacaa actctctggg tttccgatat gccttgagaa gcagagtgct
    24901 ccaaagatgg atgaagagca taacaattgc aatctgggct ccccaatggt gaagggaacg
    24961 aaaaaaccag cccatgttca gtttggtgac aatctcgatg atgctcttat gggcatgttc
    25021 aaaatgtggt acatagtaaa aagaaagaac catgcctgtg atgaattgaa tgataaaaag
    25081 aaaaagggtt ataccgcctg tgtaatacca tatttcgtat ttgtgtttag gaacagattt
    25141 ttgttttacg agatctcgta tctcgtcaat gttgtatctt tcccaaaacc agtttttaag
    25201 ccttgggcag ttcaactttt attcccttct ttgccacaat gagtttatct ccttcaatgg
    25261 tgatatcaaa aaattcaagg ggttttgggg gtggcccctc tatatttttt ccgtcctcat
    25321 caaaccatcc tttgtggcag gcacaataaa actttctatc ctgcggtaaa taggtaatgt
    25381 tacactccat gtgggtgcag atgcctttaa gtgccactgt tttaccatct gagcgtttaa
    25441 agaagagacc aagctttcca ccccacgcaa agggcttgac tgagttggca ggaatatcga
    25501 ggaaatcctc tggctttagc acaacaaaat ccggttcctt gccgagagtg gggaaggcaa
    25561 atttgataag gccataaaga ctccctccca tgaaagctgc cagccatccc ccaaaaatgg
    25621 tgtttaggaa ggttctcctt gtgacaagag aaaaatcaat cttcattttt gcccttcccg
    25681 aagagcttat ttttgaccaa ttgttttcgc aaccctacaa tggaatcggt gggtctcacg
    25741 tccttggggc aggcctcaat acatcttaat acggtgtcac agccccatac accatcctcg
    25801 ctgtcaacat tttgaaggaa attatccggc cgttcatccc gtgaatcaag catgaagcgc
    25861 tcgagcttgg caaaagcagc aggccccatg taatcggggt ttctcgcgag cacagggcat
    25921 gccccgtagc agcatgcgca gagaatgcag ttgacatatt ggtctatctt ttttctatct
    25981 tcttctgttt gagctatctc cttgtcagga taatcgccct ttcggatcat gtaaggtttc
    26041 accttttcat acatatccca gaatggagtc atgtcaacaa caagatcttt tatgatctgc
    26101 atgttaggca gcggttccag gatgatcgtg ttgctcccga aggatgctgc ctgagtccgg
    26161 caggcaagat caattttacc gttgatgacc atagcgcagg agccgcagac cgctgatctg
    26221 caagaagatc tgaaggcaag agttccatct atctcgtctc ttattctgag gagaaccatg
    26281 agtatagtca agccgggaat gacccgtatc ttataggtct gaaagtgtgg ctctatgtcc
    26341 ggttctgccg catcatacct gagaattttg aatgtgaaat agtccttatc cgtcaatatt
    26401 ttctctccat gggctcatac tttgtaatag ttacgccctt gtatgagata tttggttcac
    26461 catcttttcc aatggtggca actgtgtgct tcaaccactc tgcatccctt ctcatcggaa
    26521 aatctcttct cgaatgggca cccctgcttt cctccctcag gagagcgcct aacacgatga
    26581 tatggccaac atcaagcatg ctttccagct cccatgcccc aataagctcg tagttcatat
    26641 aaattttaga tgaagatacg tttacttttt tatatcgttc tttcagctca ataatcttgc
    26701 tcaaagcctg ctgcaactca tcctttgttc ggaagatacc ggcaagtttg ctcatggtga
    26761 gcctcaactc atcgagtatt ttgaaaggcc tttcagatcc accgctattc agttgtttta
    26821 tcttgtgctc aacctcagct tttttgctca tgagtagttt ttcatcaggt ttgaaaccgt
    26881 ctgattggag gtactgatca actgatatag aagctaattt accaaaaaca attgtgtcaa
    26941 ggagcgagtt tcccccgagc ctgttagcgc catgaacgct tacgcacgcg cattcaccag
    27001 cagcataaaa accttttacc acggtttctg acttttcatt cgtatcaatt ccacccatag
    27061 aataatgctg agaaggcata attggtatag gttcatatat cggatcaata ccaataaaat
    27121 caaggcaaat acgacggata ccaggcagcc gctccatgat cttctcggca ccgagatgtc
    27181 gaagatccag ttgaacatat tttcccagtt catgctcaaa ccctcttcct tcttcaatct
    27241 cagtctgaat acttcttgat acaatatccc gcggcgcaag ctccattgcc tttgctgcat
    27301 atcgctccat gaatcgttcg cctttgttgt taagaagata gccaccctct cccctgcagg
    27361 cttctgtcat caaaatattt gtaccaatta aagatgttgg atggaactga acaaactcca
    27421 tatctttgac agggactcct gctctgaatg gtatgccgat gccactccct gtattcgaga
    27481 gggcatttgt cgagtacaga taaacccgcc cgtatccgcc agttgcaaaa accgttgctt
    27541 ttgctgctac cggtataatt tcccccttga tctggtccag aacaattgcc ccatggcaaa
    27601 ggttgccgtc tgtcacgagt gcaaggacaa catgctctga atagactttt acgtctttgt
    27661 aaacagacct ttcataaagt gtgttaagga gaacatggcc ggttatgtcg gcagagaaac
    27721 atgttcttgg ataacctgcg ccaccgaatg gcctctgagc gattgtgttg tccggaaatc
    27781 tgctaaacgg gcatccccag tgttccattt cgtatacaat tacgggtgct tccttgcaca
    27841 taatttcaac agcatcctga tcgccgagat aatctccccc ttttacagtg tcaaaggtat
    27901 gtttttccgg agtatcatcg cgagcatcag ggttatttgc aagggcagcg ttgatcccgc
    27961 cctgagctgc tatagaatga gacctgatgg gatgaacctt agaaacaagg ccaacatcat
    28021 acttttcaca aagaccgact gctgcacgga gccctgaaag gccacctccc acaatgagga
    28081 catcgtgata aagcataatt atgacataat aaaatataaa taaatcaaaa ctgctgaaac
    28141 aattgaggcg accgtgtttg acagcaaaaa cagccgcttt tcgtttctat agccaaaatc
    28201 cattataatc gtacgaagcc cgtaaacgct atggaaggtg aaaagaataa gaattaacac
    28261 atcaaggaca ggttgtctgt gaaggctgag tgcccagtca ggtttttcat cttttgttaa
    28321 aaggaagtag cttgttagaa tcttaatacc aaagagaatt atgagagata caccactgat
    28381 tctgtgaaag agccagataa acatatcttt tgacatagat atcaaattgg ttctctgatt
    28441 gtcaagcaat attttgacaa ttacaattta taattggttt tgtacttcat tgtatctgcg
    28501 tcaaatgaaa ggatatttat cttgacaaac aacaaaccat gcgctaatac atcaaaaatc
    28561 acctgttaca atgaagaaac tgtgtattct cattttcctg atcattgcat tcctaacatt
    28621 agcaatacct tctaaaggtg ctgacaataa caaagaagtg gacagcatgc ttgtttctgc
    28681 tgagtctttt tttaaaatga tgaaagaaag aaattacgca agaatatggc ttctcctttc
    28741 ccatgcctca cagaattcaa taattgacga tacatataaa aatataatgt atcattttaa
    28801 agcaaaagga aaagaggtga catactccaa aggtgaaata agaaataact ttacaacagg
    28861 aggaaccata gcgcaggcat attgggacag ctatctgact gtttttaatc ctgacatggt
    28921 ccttgaatac agcaagtggg aaataggtaa ggttggcaaa gaaagggctc agataaatat
    28981 catatataga aaagccgaac gaccggctgt tatccaaatg tacaaggaag ataatgtatg
    29041 gagggttggg ttaacagaaa cgttcagatc tccaaaacgt taggcgtgaa gccgatgaat
    29101 acgaagaaag agaggatgaa aagccatggg tgactacgat gacaggcctg actggcggga
    29161 attagacagg agaagagaca aaagtaaact ttacgggcga atagaagaaa aagggcaaaa
    29221 gaaagaaaaa ccgaaagatc ggtatcaagc tggcagggtg aaagaggcac ttgacagact
    29281 tttcatgggc aagaaaggca caatagagca tgataagctg tataataaga ttcacagcac
    29341 ctatgggtcc aacagatttg tttctacggt aaaaaagtat attgccgaat atggtatgcc
    29401 cgataatgtc tcagcccttc ttcttattct ggatgcaaaa gaacaggata tcgtttgcag
    29461 cggcattggc aagctcaaag atctatatgg taccctgaca agcaggcaac aggaagatat
    29521 caggagaaaa ctctccatcc ttgctatgac agataagttg aaggaaatca gaaatatggc
    29581 cgcggaagca ttggaatatg taaaaagtga ggtaaaaaaa aataagtagt ttcgggaaaa
    29641 tagtgttctt tatcttttcc gggtatttgt cgaaaaggat cggaacagtc tgttccaccc
    29701 ctgctaaact cgtcacgtcc tgacaaagac gatcaggttt tgatctgcat cacccaaatc
    29761 ctaaagggta tactgctggt gggtttttaa gtacctgtga ctgggtgttc tgcaaaaacc
    29821 agttttggta tcctatgcct cagcacttta gtctgctaca tgtcagggcc tggatgccag
    29881 gcattcccct tttatttttt taacctatag cctgtaataa ccttgagata ttcagtatcc
    29941 tttatttctt tatgctataa aagacctcta cgcctccata gattgcgaga tcaccaagct
    30001 cttcctcaat ccgcataagc tggttgtatt ttgcaattct ctcgctccgt gacgcggacc
    30061 cggtctttat ctgtccgacg tttgttgcaa ctgcaagatc ggctatgaag gtgtcttctg
    30121 tttcccctga acggtgcgat ataacacatg tatatccggc ccttttcgca atttcgatag
    30181 tagtgagcgt ttccgtgaca gttccgatct gattcagctt aataagaatg gcattagcca
    30241 caccttttga aataccttcc ctgaagatat tagggtttgt tacaaataca tcatcaccta
    30301 caatctggat tctcgacgaa catttatcgg taaatttttt ccagcccttc cagtcgttct
    30361 gggcaaaacc atcctctatt gaaataatgg gataatcatt aatgatcttg tcatagaaat
    30421 caaccagctt gtcacttgtc catgtgttgc cgtcaatctt atatttacca cccgcctgat
    30481 ataattcact ggctgcgaca tcaagggcga ggagaatatc ttcaccaggt ttgaagccgg
    30541 ctttttcaat tgccttcata agggtatcaa gtgcctcttt tgtgttttta atctgaggtg
    30601 caaaaccacc ctcatcacca acaccggtag aatggccttt atccttgagc acatccttta
    30661 aagtatgaaa aacttcaact cccatcctga tagattctct gaaacaatca gtacctacag
    30721 ggacaatcat aaattcctga caatcgagcg agttctgggc atgagcaccg ccattaataa
    30781 cgttcatcat gggaacagga agttccctgg cacgtatacc acctatatat tgatagaggg
    30841 gaagaccgac gaagtctgct gctgccctcg ccacggccat ggagacgccg agaatagcat
    30901 ttgccccgag attgctcttg ttttccgttc catcgagttc aatcatgaga ttatcgatac
    30961 ttgcctgttc cagcgcgtca aggccttcga gcttaggtgc tattgtctca ttaacgtttt
    31021 ctacagcctt caaaacaccc tttcctttgt atctcttctt gtcaccatct cttagttcaa
    31081 gcgcttctct ctcacctgta gatgcacctg atggtacgag agcccgaccc atagagccgc
    31141 tctcaagagt aacctcaacc tcaacggttg gatttcccct gctgtcaagt acttccctag
    31201 caaaaatgtc gtatatggat gacataaaat acctcctttt ggaattcagt ttttggcgtg
    31261 ctcataagtg tcggcagaaa gtgaaatacc ttactcccta actgccagac acaatatatt
    31321 tgagaatacc acaagaagat gaactgcttc aaatgattta tgctcggtac agcggtaaaa
    31381 cgattctacg gtgtattttg tgtattatgg ctgccaattg ttcaggtgcc gttatattaa
    31441 ttgaaatcga aataggtaaa taaaaaaaat gattcaattt gcgaattatg cttactcgtt
    31501 atatattcat agctgattgg aggaatggtt ggaatacacc agactttttg ctttaaaact
    31561 cttacagtct gaaggacggg ccttagaacg actttacagc aatgccgata tggtccgtcg
    31621 gaaaaagatg ggtgatgacg tgttcattcg gggtattgta gagttctcaa acgtttgctc
    31681 aaataactgt ctctactgtg gcatcagggc atcaaacaga aacgtgagaa ggtactccat
    31741 gacaaccgaa gaaatcatgg aggctgccat agctatggag gaaaataatt tttcgaccat
    31801 tgttctccaa tctggtgaaa ttcgcgacat taaggatgat gagataggag aaattattcg
    31861 gaagatcaaa aaaaagacat cccttgccgt aaccgtgtct gtgggaaatc gcaggaaaga
    31921 aacatatagt ttttggcgtg attgcggcat ggaccgttat tttctgcgct tcgaaacaag
    31981 tgatgcagaa ctattcaaca ggctacatcc tggatgtacc ctaaaagaga gattaacctg
    32041 ccttgatgat ttgcatgacc ttgaaataca gaccggtagt ggatttatga taggacttcc
    32101 tggtgagaaa cttgaaactc ttgcagataa cattcttcta tgtcgtaagt ttgatttcga
    32161 tatgatcgga attggtcctt ttatacctca ccctgagact cctctgggaa atagcccaaa
    32221 tgcctatagt gacaatccag agttattttt taaggccctt gctgttctcc gtcttttcaa
    32281 tccggaagcc catattcctg caacaaccgc atttgatgca gttttcccag gagaaggcag
    32341 gaccttggcg cttcaaagag gggccaatat ctttatgcct aacaatacgc ccgtgaaata
    32401 ccgtgaggac tatcttcttt accctggcaa gccaggggta gatgaggatc ctgaacaatg
    32461 cctgagcagt gcggttgtga gaattaaatc tttaggcagg ccactgggga agggaccggg
    32521 tcactctatt aaaaaaagac acagagagaa aaggtccaag gattgaggcc caataacgtt
    32581 gataacattt tcagtctgtc catcaaccct gcacctttat cctcgaacct tttttcctgt
    32641 atcttttttt aaatatattt tcctgaaata tagtcactca gatgttcaat catcgtatcc
    32701 cgtgctatca acattgattt gacaacatca ccaattgaaa ctagtcctac gaatttatca
    32761 ccttcaaaaa ctgggaggtg tcgtatgcgt ttgccggtaa taaggaccat acagtcttca
    32821 acagaggtct caggttttac ggtatacatt ttttcgaaag gtgtcataat ctcttcaaca
    32881 agagtttccc gcgatgtttt tcccctgaga ataaccttcc ttgcatagtc tcgttctgaa
    32941 agaattccga ccactttctc attttcatct acaaccatta cgctaccgat ttccttttcc
    33001 cccataatct ttagtgcctc aaaaacggtc gactttggga ggacggaaat gactgctttt
    33061 tttctttccc taagtaagtc tttaacagtt tgcataaagc tacctcctta tagatatatt
    33121 aacccgatct attgaagatc ttacagataa aggtcacgct ccccctgttc gagtttcttg
    33181 attcttttta ttgtctcatc cctgatttga gtattcggaa tttgttccaa atgtttttca
    33241 attgtctttt cacctatctc acgggtttcc gcggaggcat agtcgagaag aaactccttg
    33301 aaagtgagaa ttgcatttgg ttgacagaga ttctgaatct cgccggattt tgcaagtggc
    33361 ataaagcggt caccggttct acctcttcgg taacatgccg tgcagaaact tggtatatag
    33421 ccagattcac aaacacttct taatacttca tcagtggtgc gatggtcttc aacttgaaac
    33481 tgaccaacag tctcaccagg ttttttctca atatctgtgt ggtaacctcc aacaccggtg
    33541 caagaacctg cactcaattg tgagatacca agttcaataa cctgatccct gaattctggt
    33601 ttttcacgtg tagataagat gatacctgtg tagggtactg ccagacggat gattgataca
    33661 atctttttga aatcttcatc gcttacgagg tagggaaatt tctcaagggt tattccttct
    33721 gcaggacgga ttcttggcac tgagatagta tgacagccaa caccaaaacg ctcttcaaga
    33781 tgcagggaat gtagaaggag tgcaagaact tcaaatttgt aatcataaag accaaaaagc
    33841 acaccaaaac caacatcgtc tataccacct tccatagctc tatccatggc agttgcgtgc
    33901 caatcataat ctctttttgg accggagggg tgcatttttt tataggtttc cctatgatag
    33961 gtttcttgga aaaggatata tgttccgatg cctacttcct ttaatttctg ataatcttca
    34021 accgtagttg ctgcaatatt gacgtttacc cggcggatac tccctgtctt atccttgacc
    34081 tcataaatct ttttgattga attcaggaca tattcaatgg gacaattaac agggtcttcc
    34141 cctgtttcaa gggcaagccg cttgtggccc atggtctcga gagcaataat ctcattctct
    34201 atttcgttga aatcgaggcg acgacgaact atagtattac ttttccggta gccgcaatag
    34261 gtgcagttat tgatgcagta gttgctgatg tagagcggtg cgaagaagac aagacgtttt
    34321 ccgtatatct tttgtttcac ctcatgggca gcttcaaaaa ggaggctctt tatttctgct
    34381 tcatcgcact gaagtagcac tgcagtttcg taaggagtga gtccttttgc atcccgtcct
    34441 ttttgaataa tttgacgtgc ttcttgctga ggtgcagatt ttgctctttc cagaaggtta
    34501 aaaatatatt tttcatcgat aaaatctgct ttatactttt tatcttgcct tttcattaaa
    34561 tcgtattctc ctccgtactc aaatatattg atagacgatt caaaagtaaa tagaatttac
    34621 gtttttttgt tctcaagaac agtaagggcc tgaggaaacg gtgcaagtgc gcgttttaat
    34681 attccgagaa cataagcaat gattactcca taattaacca tggggacccc ggcttgtttt
    34741 gccatcatga ggcggtggag catttctttg cggttaatca tacacgctcc gcaatgaatt
    34801 ataagtttgt aatcctcaat atcggctggt aattcatggc cacttgccca cttaaaatta
    34861 agcggacctc ctacaatctg ctgcaaccac cggggaatct tcactgttcc gatatcgtct
    34921 tcgactctgt gatgggtaca ggcctctgca ataagcactt tatcaccggg aacaagatct
    34981 tccaccacct ttgcaccctc tactagagag atcaaatctc ctttattgcg tgcaaagaga
    35041 atggaaaaag aggtcatagg aacgtcaggg ggggtgtctg ctgctacttt aagaaaagcc
    35101 tgggagtctg taacaacgag ccgaggttta tttttgagat tctcaatagc agctttaagc
    35161 tcttgttctt tgacgatcag ggcaattgcg tcatgatcaa ggatatctcg tattgtctgt
    35221 acctggggaa ggatcaaccg ccccttcggt gctgcaagat caattggtac aacaaggatg
    35281 actgtatcac cgggtgagat aagatctccg ataatagtgg atgacatcca gtctttaggt
    35341 gcaaccttga tcatttcgag cttcagttcg tccattcctg ttcgagtgac ggcgcttacc
    35401 ggtaaaacag gtatattgag tatggaacta atctcctctg gagaagcatc ggggaagata
    35461 tctatcttgt taaggacacc gataatagca accttttgat cccttgcttt ttgaatgact
    35521 tccagttcat gtgagtcgac ccctttggaa ggatcaatga cgaggagaag gatatctgtc
    35581 tttgcgagaa cattatatgc cttttcaatc ctcagtaagc caagctcacc tgtatcatca
    35641 atgcccgcag tatcgatgat gaccactgga cctacaggaa gtatttccat ggatttataa
    35701 acgggatcag tcgtagttcc tggtacatct gagacaattg caatattctg gtttgtcaat
    35761 gcattgataa tgcttgattt ccctgcattt ctccgaccaa aaatagctat gtgaagcctg
    35821 ctgccacgtg gagtctggtt aagtgttgta tcttccattt attggcttcc tttcgttggg
    35881 cctttaagtt tacaaagctt cgtattagcc agcatatgta aaagttcgac tggaacacag
    35941 aagtatgaaa cgaggaagtc gtatactgtc cttactgagt ggaaaggata gtaattgttt
    36001 tctgaaatga gggttttgcc caagcgatcc tttgtaagac gggtcataat aagagaaggt
    36061 atttttcaca cgcttatgac aaaaaaagag cacctgaacc agaggcggat actcttttta
    36121 acaaaaacac atctcggcat ggactaactt caaaattaga acctactcct tggtgtatag
    36181 tgggtgtgaa gaagcttatg agatttcttt ccaaggggtt ctttgaggaa ttgtttgtaa
    36241 attttcttga ttgccggatt ctcatgtgat tttctatatt tgagggcaga atcttcctga
    36301 taaagggctt gtgctcgaag actccgtatt tcatcgttta ccgggaatgg ctgcccacct
    36361 ccaccaacac atcctcccgg acatgccatc acttctataa aatggtactg agtactgtct
    36421 tctttgactt tttcaagaat tttccgtgca ttcccgaggc catgagcaac cgctactctt
    36481 aggtccccaa ttccttccac aggaactaca gcttccttaa gaccctgaag gcctcttact
    36541 gcagtaaatt cgaggttctc gagggttttt ccggttgcca cctcatatgc tgttctgagt
    36601 gctgcttcca taacaccacc ggttgcacca aagatcgtgg ccgcgccagt atattccccc
    36661 atgggatcat catagtaacc gtctggtaaa ttgacaaaat caattccagc ctgttttatc
    36721 atttttgcta actctcttgt agtaagaaca taatcaacat ctttatagcc gctattgctc
    36781 atctcagggc ggttgcactc aaattttttc gcagtacacg gcatgatgga gaccactacc
    36841 atatccttgg gattgacttt tgcttttttg gcaaaatatg tttttgaaag tgcaccgagc
    36901 atctggtggg gagatttaca tgtggagaga tggggaagga gttcaggata aaaatgttca
    36961 ataaatttta tccatcccgg gctacagctt gttattaggg gtagggttcc accttctctg
    37021 atgcgcttca gtaattctga accctcttca agaatagtca ggtctgcagc gaaatttgta
    37081 tcaaaaacac gatcaaaccc gagcattctc actgctgcaa gcatttttcc tgttatcagt
    37141 gtgccaggcg ggtatccaaa ttcttcgccc agtgcagcac gaacagctgg tgcgtcctgg
    37201 gcgataacga atttggtagg atcgttgatc gcttcaaaga tttcgtcgat atactccttt
    37261 tctgtgattg cgccaacagg acataccatg gaacattgtc cacaattggt acatgcaact
    37321 tcattgatat ctttaccaaa agcaggctca acccttgctt cgaagcctct gccaacggta
    37381 taaagggcag tcaccgattg aatttcttgg caaacggtaa cacaacgtct gcacaggata
    37441 catttgctct gatcccttat tatggaaggt gaagagtcat caatacgttg ggcgcttttt
    37501 tcgcctgtaa accttacttc cctgatctgc agttcggagg caaggttttg aagctcgcaa
    37561 tttagatttt ttgcacaggt aagacattca aatgggtggt ttgagaggag catttccaca
    37621 gagaatctgc gtgcctttct aactcgttct gtgttggtat tgacaatcat gccgttggtg
    37681 gccggtgcca tacaggatgc ttggagtgct ttattaccct caacctcaac aagacaaacg
    37741 cggcaggctc caatagcctg gacttctggc aggtaacaga gggttggaat atggatgttg
    37801 gctttaagcg cggcttcgag tatagtagag ccttcctcaa cttctacctt cctgccgtca
    37861 attgttaatg tcatcatagg aatatactcc ttatttgttc atgggaaatt attcttgttc
    37921 tcttagataa caatggagac agcgcttggc ttcctcaact gcggcattcg cctggtagcc
    37981 aagtgcaacc tcattaaagt tcctgaatct cttggcgaga ggtaaatggg acatctccat
    38041 cctttttctt tctttctggt attctgcctc atcgtatgaa acaaccatag tgataagttc
    38101 atcccggaaa gcgtttggcg ctaagccatc acctccaaaa tgaacatcta tagccatagc
    38161 agctttttta ccatcagcta cagcctcaac tactgatgca gggcctcgca cattatcacc
    38221 ggcagcatag aagccttctt ttccagctga aagggtatac ttgtcaactt caaccgtatt
    38281 gttattggta accttgatcc cttcgccatt aagataagag agatctggca attgacctat
    38341 tgctgcaatg accatatcag catcaatagt gaacccggag ccttctataa cgtgtggact
    38401 tctgcgaccg ctcttgtcaa atttaccaag ggccattcga gtacattcga cacctgtaac
    38461 ttttccattt tttgaaatga ttttgtcagg tgcgacaagt gtatgaatct tgaccccctc
    38521 atgttctgct tcatcgatct cctcagcgta ggcaggcata tcatcacgct ctctcctgta
    38581 aagaactgat acttcctcgg caccgagcct tagtgctgct ctggctgcat cgatagcaac
    38641 gtttccacca ccgatcacta cgactttgcc ttctgttttt actatttcat tcaggttcac
    38701 agcacgaagg aaggtaacac catctgctac tcctgatgct tcctctcccg gtataccgag
    38761 tttctgtcct ttatgggcgc ctgtggcaag aagaattgcc ccataaccat ccttaaacag
    38821 actttctgag gttatgtctt taccaaattg agtattgtac tttatctcca cacctatatc
    38881 tgtgatggat ttgatttctg cgtttaaaat atcccttggt aatcggtagt cagggattcc
    38941 cacggcaagc atgcctccag gtacggagag ggcttcaaac accgttggtt tatatccttt
    39001 caaggcaaga aaatatgcag cactcagacc ggcaggacca gcacctataa ttgcaacttt
    39061 tatgtcattg gactttgctg tcacaggcct gaatgacggg aatgtagcca tgtcgacgtc
    39121 agctgcaaat cgcttgagcg cacagatagc tactggctgg tcgatctggg ctcttctgca
    39181 tttgctttcg caggggtgat agcaaaccct tccaagaact gcagggaatg gattagcttc
    39241 ctttatgagt gcaattgcct ccttaaattt tccttcgctt ataagggtta cataacccca
    39301 ggcattctga tcagtgggac atgcgttctg acagggggca tcaaacaatg aactgcatac
    39361 gccagcactg cagtgtttat ccacgatgtg ctcaacgtac tcatctttga aaaaacggag
    39421 tgttgaaagg acagggttcg gtgcagtctg ccccagacca cacagtgctg catctttaat
    39481 gcgagtggcg agttctataa ggttgtcaat atcctccaac ttgccttctc catgagatat
    39541 ccgtttcaga atctcgagca tcctttttgt accgatacga caaggggggc attttccaca
    39601 ggattcctcc tgaataaaat ccacgaagaa tcgggcaaag tctaccatac aggtatcttc
    39661 atcggccact attagaccgc cagaaccaac aattgccccg agttcagtta ttgattcata
    39721 atcaacggga acgttgagat gttcaacggg gatacagcct cctgaaggac cgcccaactg
    39781 ggctgccttg tattttttct tgttttttat gccaccgcca acattaaaaa tgatatcacc
    39841 aaggggggtt cccatgggaa cctcaacgag acccgtgttg ttcactgccc cggcaagggc
    39901 aaatactttt gtacctttgc tcttctctgt tccgtattga gaataccagg cagccccgtt
    39961 catgataatc gatggtatat ttgcataagt ctcaacgttg tttaaaagag taggcacatt
    40021 gaagagaccc ttcacaacag gaaatggcgg tcttggtcga ggctcacctc tttttccttc
    40081 gatagaaaac atcaaggcag tctcttcacc acaaacgaaa gcacctgcgc ctatgcgaat
    40141 gtcaaggtca aaatcgaaat ctgtgtcaag gatttttttg ccgaggaact tatattctct
    40201 ggcctgaccg attgcccaga ccagccgctc aatagcgaga gggtattcag ctctgatata
    40261 aacgtaaccc tgcttggcac ccacggcata ggcggcaata gccatagctt cgattacact
    40321 gtgaggatct ccctcgagga cactcctgtc catgaaagct ccaggatctc cttcatctgc
    40381 gttacagaca acgtatttca catctcctgg agattgatat gcaaatttcc atttcatgcc
    40441 cgttggaaag ccaccaccac cgcggcccct caggccagac tttaacacct catcaatgac
    40501 ttcttccctg gtcattgagg taagggcttt cgcaagtgca aaataaccat cgcgggcaat
    40561 atattcatct atgctctgag ggtttataac tccgcaattt cttaaaacaa tctttacctg
    40621 atttttgaag aaatctatct ctttcaggga tggtatggtt ttggctgtaa caggctcctt
    40681 ataaagaagg cgttcaacca ctcttccctt caacaggtgt tctactacga tctcctctgc
    40741 atcctgaggc gtcaacttct gatacaggac tccttcagga tatatgagcg cgagagggcc
    40801 gagatcgcag gaaccgatac atcctgtttc gataacctta atctcatctt gcaatccctg
    40861 ttcaacgatt ttattgacta cagcgtctct aatttcgcgg caaccagaag agacacatgc
    40921 agctcctgca caaaccaata cgtgcgcacg tgatgactcc atacctatga accctcctta
    40981 ctcatacttt tcgagaatgt ttgcaagatt cgttgactta acacgtccat agacgtcatc
    41041 atctatgagc atcgcagggg ccaatccaca tgcccctata caacgaacaa catccaggga
    41101 atatctcctg tcctctgtag taccgcctac ttttataccg aggtctttct gtgccttatc
    41161 aagaacttgt tgcgaaccgc gcacatagca tgctgttcca agacatattt tcacgctgtg
    41221 tcttccctgg ggcacagtgg agaagaaatt gtaaaaggtg atgacaccat acacctcact
    41281 aagtgagata ttcaaacctt cagcaacctt ttcctgaacc tttctgggca ggtatccaaa
    41341 gagcccctgc gctttgtgaa gcaccatgat gagatcttca ggccttccgc tatgttctgc
    41401 gatgatttca tccaatctgg gatacagctt tgcttcttgc atctcagcag aactctggtt
    41461 gttgtcagta gccatgttat tcctccctca cattcatcta cgtaaaaatg attattcgtt
    41521 tatcactttt ccatccacaa gacttctctt catataatgg gtatgaagga gttcgtgggc
    41581 cttcttggaa ttcggttttt caaggaattt ttcgtacact gcctgaacag accggttctc
    41641 gtgtgacctt ctatgggcca ttgatttgtc ttctgcgtaa agagcttctc ctcttcgtgc
    41701 ccgcttggcc atattgctac cccatgactg cccaccacca ccaacacatc ctccgggaca
    41761 tgccatgacc tcaatgaact ggtattcaca cttgccgtct gtagccatct gattttggac
    41821 tttttcaagt agctccctgg cctgagcgcc gctatgtgcc acagcaactt ttaccttggt
    41881 accatcaata tcaatctctg cctcttttat accctttaat ccccttacgg gtttaatgtc
    41941 gagattttca agctcacggc ccgtaaccag aaagtatgca gaccgtatcg cggcttccat
    42001 gactccacca gtggctccaa aaatggttcc cgcgcctgta tattctccca taatgtcatc
    42061 cgccagttct tcgggcatct ccttcaaatc aattcctgct tcccgaatca tcctgccaat
    42121 ctctcttgtg gtaagaacat aatcaacatc ctggtagccg cttgccttca tttccggtct
    42181 ctgacactca aatttctttg ctgtacaggg cataacggat acagagacta tctttgcagg
    42241 atctatgttt tttacctgag cataataggt tttagcaagt gcgccgaaca tttgctgtgg
    42301 agattttgcc gtggacatat gtgggatcat atcgtggaaa aaggtttcca taaattttat
    42361 ccatcctggg ctacaagagg tgataagggg gagattcttt ccagatttta agcgtgatac
    42421 caactctgtt ccttcctcaa taatggttaa atccgcacta aaatttgtgt ccatgaccgc
    42481 atcaaagccg agtctcctca gggctgcata catttttcct gttacgagag aaccgggtgg
    42541 cattccaaat tcttcaccca gcattgctct caccgcgggc gcctcctgaa caaccacaaa
    42601 tttatctgga tcgtgcaggg cctcccagac agcctcaaca gagctatttt cgtaaagagc
    42661 accaaccggg caatgcacca cacactgtcc gcagtttata cacacactgt taccgagacc
    42721 tgcactgaag gctgtaccga tgactgtgtc tgcgcctcta tgattcaccg ctaatgcatt
    42781 gaccgactgc acatcagagc acacctgaat gcacctgcca cagaggatgc acttgttcgg
    42841 atctctaacg atagaaatgc ttgaatgatc tataggtaat ttttcttcaa gtttggcata
    42901 ctcgactatt ttgataccga gggtagcggc aagcgtctga agctcacaat tattgttttt
    42961 tatgcaggtc aggtaatcag aatcatggtt ggcaagaatc atctcaacaa tctcttttct
    43021 tgcactcata accttttgtg tgtgggtgta taccctgaac ccttcgccaa tgggagtcat
    43081 gcaggccctc ttcagacccg ggataccttc aatttctaca acacatacac cgcactgacc
    43141 acctggcttt aaatcaggat gaaaacataa ggttggtatg ttgacatttg ccttcttggc
    43201 agcccacagg atagatgttc cttcagggac ctctattttt acattgttta tcgttgcttt
    43261 tatcattgtc gattttgttg cctccttgtt ggctggattg gttaaagtta gtgaatgcgg
    43321 gcgtcaattt cgtctctgaa atacttaagt gaacttgaaa tgggcagatg taaggattgt
    43381 ccgagagggc agaaggaagt gtcccgcatt gtatctgaaa ttttaagaag cttatctaca
    43441 tcgccttgaa ttcctttaga ggctgcaatt cgatcgatta tcttagcaag ttgagcagtt
    43501 ccgagtctgc atggaacaca atggccacac gactcgtgtc ggaaaaaatg aagcacactc
    43561 tgtaacatat cgactatatc agcggtttcg tccatgacaa gaatggcccc ggatccaaga
    43621 acagctgcgt attctctgag attcacaaaa tccatctgaa cgtcgagcat atcaggaccg
    43681 agaaacgcac ccgcagcccc tcctatgagc gcgcccttaa atttcttacc agcctttatt
    43741 ccacctgcat attcaaaaat aatatctctc aaagttgttc ccatctccgc ttcaatgaga
    43801 ccaggtgtag caacatttcc tatgatggtg tacacttttg tgcccggaca tttttctgtg
    43861 ccgtaacccc tgaaccaggc cgctccattc ttgataattt caggaacgtt tgaaagagtt
    43921 tcaacgttgt ttaccacagt tggttttccc cagagacctt ctgtaacagg gtaggggggt
    43981 ttattccgtg gatggccacg tttaccctca agtgactcta taagagcggt ttcttcgccg
    44041 cagacataag caccagcacc tttcatgaca gatatgtcaa aactaaacgt gctgtcaagg
    44101 atattattcc caaggagatg atattccctt gcctgagcta ttgccttctc aagacgttct
    44161 attgagagtg tatactctcc cctgatatag acaaaaccct ggcttgctcc cacggcgtag
    44221 cctgccagca tcattccttc aatgagtttg tgggggtcgc cttcgagaat aagcctgtct
    44281 ttaaaggttc ccggttcacc ttcatctgca ttacagatga tatatttaat atcaccctgt
    44341 gcgcgtcttg caaagtccca tttcatgcct gttgagaagg atgcaccacc cctgccagca
    44401 agtcccgatt tttttatttc atcgatgatg tcttcaggct tcatggcagt cagtgctttg
    44461 ccgattgcct catatccgcc ctcggctata tactcttcga tcttttcggg atcaataacg
    44521 ccacaatttc gtaaaacaat ccgtggctgt tctttgagta ggcccgcttt tcctttttta
    44581 ataactgttt gtttaggtgc ttcggatagt attaaccttt ttaaaggtct tcccttgagc
    44641 agatgttctt ccactaattc gggcacatcc tgaggcgtaa cgtgagcgta ataaacgcct
    44701 tctgggtaaa caacgaggac tacgccctga tttattgtac cgatacttcc agtctcaaga
    44761 actgctatct cgtctgaaag accctgtttt gcaatctctt caatgagtgt tttttcaacg
    44821 gcccgtgcac ctgccaacac agtgcctgca tctatactga tcagtacatg gtttctcaca
    44881 aggctcatga ctcttgtctc cttttttcaa gaatggcgtc aaccttttcc ggtgtcaggt
    44941 taccaaccat ctcgtcattg atcatcattg ccggtgctac tccacagaca ccgaggcagc
    45001 tggtaagctc gagtgtgaat ttgccatctt ttgttgttcc tccaacatcg acctttaaag
    45061 ctttttttag atagtctaat aatgatttgg aacccataag atgacaggga ggcgaatcac
    45121 agagccttat gatgttactt cccctcggtt cgaggctgaa catggtatag aaactcgcaa
    45181 cactgtgaac ataactgtag ggaactttga gataatctgc acatactcgg atatcctctt
    45241 tcgctaaata gcgttgtgga ttattgtcct gaagatcgtg aagaatataa agaatgttat
    45301 caaggcttgg tttgaatttt tttaaaatag cttctctgta caagtttgtc cccctccttt
    45361 acataacctc aagtgcaaat tctgtaactg gattgccatt taccacatgt tcaaccacca
    45421 ctcttttagc tctttcctgg ttcatgttgc catacacaac caacggctta ccttcttcgt
    45481 aaacctccac gagtggttcc tgtgaagcca accccttttc accggtctgg gttacgatta
    45541 cgtcagtaag attccttctc ccaatctctt caagaagggt tttgaggaca tctcgtgcac
    45601 cggctgctat tccactcgtg cccattccta cgacaatccg tattctagcc tgcttctgcc
    45661 gcaattctac atctttttgc gccttttctc tgattttctt cagttcttct aggttcatcg
    45721 atttgcctcc tttaaattct agtgaattgt acaagagttc acatgttttc gtcaatacat
    45781 ttagttaaaa aagtgattac ctcgggatga ttaataggaa caccatccaa gaggttttta
    45841 atctcttttg tatccaacgt aaattctttt gcatctgctt ttatacaaaa gcaaaaatca
    45901 gtgtccggat tccccacaac aagtacaagt attgtatcac gaatatttcc taggggagga
    45961 agatcaggat gactcaaatc aaaagaagca aagatttctg taccctgtcc cataatgctc
    46021 gaaaggcaaa aggtaccgtt tgtcgtttct gctgttcctt ttaggagggg gatgccgagc
    46081 cctaccttct tgcctttttt tgttgtatag aagggatcga ggactttttg tttcgttgtc
    46141 tcgtccatac ccacaccatt atccttgata cgaagggtca ggagattttt ttcagtatcc
    46201 tgtgtaatat cgacactgat atgtgttgcc tttgcagaca cagaattggc aacaatatca
    46261 ataatatgtg aagcaatttc atccatcatg ggagtgcacc gccaatatat tgtttctgaa
    46321 gccctctaat tttctatgca acctcctttc cagtttatat ccgcagccta gggtagcagc
    46381 tatatttatc aaactagcct aaaataaaca aatattttat ttattgtaat taattcatta
    46441 tattttaatg aataaataat actaccacca tggttctttt gtttacatgg gtggctccaa
    46501 aggtcacatt tttaggaaat atttggcctc tgccataaaa tagtggtcat agtcggatat
    46561 attcataaca actgcttcag ggcatttgcg atttctgaac aatttggctt ttttagatca
    46621 agactaatcc ttcgcctacc aatgtctttc aaataatggg catcggaaaa agtgacaaaa
    46681 ggtatatctt ttttcataat aagtggcagc agatcgatta ttttctcctt atttcgtact
    46741 tcaagtgcat cgaaaggcaa atcatccggt atgtagccaa gttggctgat aatgctgaag
    46801 gtagggctgt caatatgaga aggaatggct aggccaccgt ggtctttgat ccattttgtt
    46861 gcatcggtaa ttgaaatctg agctgaattc aaaaggagtc tggtctcgaa tcggataatt
    46921 tcgttttcct catccaccac aacctggtct ccaaaatatt cggcatcatt ttgaatgtca
    46981 ggcagaagat tatagatctt atcctgaaag gtatgagcaa gatcatagtt gtcgaaaaat
    47041 gctaaaagat gaatttcttc tgaggtctgt acctctatac caaaaagtac cgtaataccg
    47101 aatttttttc ctattgtata agtaggcaag ctgttttcca ccatattatg atcagtaaca
    47161 gagatcagat gaagaccaac ctcttttgcc tttgctatga tattgttagg agacatttca
    47221 aggcttccgc agggtgacag ggtcgaatgg atatgaagat cacatgagta agtcgacaac
    47281 tactctagcc tttttgtaag agattgtata taagaccggc aagttcaaag gcggagcggg
    47341 ggtttctgag gatggcaatc ctctcttcac tggctttttc aatcatactg tcttcaagct
    47401 gtagattcct tggtattaga atagcagcaa catcctttaa ctttgcaaca ccaagaatat
    47461 tcatatgcct ttgtatagtt atccagacag agttttcttt aatgttgcca ataacatccg
    47521 atagaaggtc cgacacataa ccatcttcta tgtctttgtc taagtttaca tttcctgtta
    47581 atatctcaca tcctgttttc ttggcaagat cagagagctt catttttcct cctc
//
LOCUS       DAOOMC010000004        41449 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdus sp. isolate AMR_MDS_2685
            NODE_697_length_41449_cov_6.410325, whole genome shotgun sequence.
ACCESSION   DAOOMC010000004 DAOOMC010000000
VERSION     DAOOMC010000004.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864760
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdus sp. (wastewater metagenome)
  ORGANISM  Syntrophorhabdus sp.
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae; Syntrophorhabdus.
REFERENCE   1  (bases 1 to 41449)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 41449)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,729
            Genes (coding)                    :: 2,708
            CDSs (with protein)               :: 2,708
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 7 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41449
                     /organism="Syntrophorhabdus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_697_length_41449_cov_6.410325"
                     /isolate="AMR_MDS_2685"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2699756"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..632
                     /locus_tag="PK125_00765"
     CDS             <1..632
                     /locus_tag="PK125_00765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB36670.1"
                     /translation="LGLSRYTMMKARKHLRKLGIIETELHMANGHATTHYKVNFRRFR
                     TAFQEFIESQKVHTTNETELEPGIYTETEPMDVDDWPGDVYSMAEEMDPNQLITLEDY
                     CASAFLGSPVKLMVSLFRLKTGKDHPKLTLSEFSKLEAELPICSDGNETVRIDDDKII
                     IEMLIEYTRTAYDDPAAYTFQHFNKPEVKTPLYKKVMEQRKESMEANND"
     gene            625..867
                     /locus_tag="PK125_00770"
     CDS             625..867
                     /locus_tag="PK125_00770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB36671.1"
                     /translation="MIKNEIKSNSGIGKKAKGRGELVRHRRGERLTLKEAVLANCYEC
                     MGGYTDGLCDCEIPDCPLYQFMPYKESKREGAEHHE"
     gene            860..1105
                     /locus_tag="PK125_00775"
     CDS             860..1105
                     /locus_tag="PK125_00775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB36672.1"
                     /translation="MSENRIKVVKEIGRKANGKSKLLKHLRGENLTIWEAVKAHCYDC
                     TRYYTNPEPWDCKNLECTLYPFHLYNKEYMKKARGLD"
     gene            1394..1756
                     /locus_tag="PK125_00780"
     CDS             1394..1756
                     /locus_tag="PK125_00780"
                     /inference="COORDINATES: protein motif:HMM:NF024139.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HPB36673.1"
                     /translation="MNIDFQLSDQDIDSIVDKLEKRLRHLLSKQSYTSDNEYLDVKSL
                     SEYLHVSKSWVYERTHLNEIPFIKLPSQQGKRSNGTTSGKVLFKRSAIDAWLERNSVP
                     EISQSKNGLNLLKGGKAS"
     gene            2094..3167
                     /locus_tag="PK125_00785"
     CDS             2094..3167
                     /locus_tag="PK125_00785"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HPB36674.1"
                     /translation="MAKTKGIYKRGNIYWIRYAGPDGRIIRESTGSSKIKDAESKLID
                     RRKEVKEGELPEIHKIENHIFRELADAYLVWAERQRCFNGKKYLIKQLVDKFGNLPLR
                     RFNTMLVEQFQTERMQKGNKPATINRFLATLKHMFTKAVDWNMVEESVLKGIRKVKLL
                     QENNKRLRFLSKEECQLLLNSCDNHLKPIVFTALHTGMRKAEILNLCWNQVDLKHGFI
                     LLEITKNGERREVPINEPLQEMFQRQTRRLDVPFVFYDNRNGQPYQDIKHSFASACRR
                     AGIKDFHFHDLRHTFASHLVMAGVDLATVKELLGHKTLTMTLRYAHLAPSHKVKAVDI
                     LAERLTQESTAQKVHNLEIVQNG"
     gene            complement(3191..3266)
                     /locus_tag="PK125_00790"
     tRNA            complement(3191..3266)
                     /locus_tag="PK125_00790"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3231..3233),aa:Ala,seq:ggc)
     gene            complement(3551..4369)
                     /locus_tag="PK125_00795"
     CDS             complement(3551..4369)
                     /locus_tag="PK125_00795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB36675.1"
                     /translation="MKKHIKITVAVLISIIFASTGFAFSKVPPEKSSKENLVTEKVPA
                     PQAPGTSENTATLKKIMTEPKISGFVGTATLIEENLMQVKDKKEVVTFDTSNPVLKGY
                     KDLSDVVVGDTVAVEYTKDGIKITKLRGTTKAKALVKKKTERVKKSPKRIALTKIIAE
                     TKGSGFVGTVTMVYSNLIDVLSKKGEVVTFDTSNPVLKGYKDLSDVVVGDTVAVEYTK
                     DGIKITKLRGTTKAKALVKKKTEMETKSPRKTVVTRVTCTGKGPCAVSVDKKAD"
     gene            complement(5608..7152)
                     /locus_tag="PK125_00800"
     CDS             complement(5608..7152)
                     /locus_tag="PK125_00800"
                     /inference="COORDINATES: protein motif:HMM:NF004837.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HPB36676.1"
                     /translation="MIEKMVLNIGCWIEKWARIQPKKIALISEGVPYTYRNLNQRINR
                     LSNFLLHMGMEKGDRVAVLLRNCRQYIEIFLALSKTGGILVPLNWRLALPETEFIVRD
                     SSPRFIIFDEEFMGNAAMLREKLPVFKEIFCTSGENKRVCEVPTWAIDYESSIMTYPD
                     SEPNIPWESGAQDPHIIMYTSGTTGLPKGAILSHQKTFFNVLNAGIYFDLTSHDIVMV
                     ARPMFHSGGLIVELAPILYRGGTVIVRKRFSPQEILETVENERVTVLELPATVYNIIL
                     NECDISKFDLSSVKCFFTGGERIPVALLKAYAEKGIIISQIYGLTEVSTIFWLPAGRA
                     IEKMGSVGQPAFHGEVKIVDGKGKPVKAGEIGEIVVKGPIVMNGYWKRPDLTRQVMRG
                     GWLQTGDLARFDEDGFAYIVDRKKDMFISGGENVYPAEVEKALFSHPSVLDAAVIGVT
                     DKKWGEVGKAFIVPKEGCSISADEIRSFLEDKLARYKIPQHIEFIKKLPKTASGKIKK
                     NLLKSF"
     gene            complement(7213..7863)
                     /locus_tag="PK125_00805"
     CDS             complement(7213..7863)
                     /locus_tag="PK125_00805"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HPB36677.1"
                     /translation="MIKGVIFDFDGTLTELTIDFPLVRGEIEKVARRYVGDDEIKAQE
                     GQYILEMINGVEEKLGGLVPDFKREAFEELRRLELMASEGKDVFPYTREVLTKLKDKG
                     IRVGIFTRTHLDVVLQVFPDIGDFVEAIVTRDDVKKVKPHPDHACEVLRLIGVRPEEA
                     LFVGDHPTDIQSGLAVSAKSAAVLTGRITRETFEDVGATYILNDVRGILNVLDKDR"
     gene            complement(8130..8399)
                     /locus_tag="PK125_00810"
     CDS             complement(8130..8399)
                     /locus_tag="PK125_00810"
                     /inference="COORDINATES: protein motif:HMM:NF014841.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HPB36678.1"
                     /translation="MTIINVARGSIFVEKGLLDILDSGQVGFASLDAFDENPSLKENL
                     LVLSQKTICMPHPEASTKEAQDKVNNIYFKTDLRGHMLFIYNYDT"
     gene            complement(8724..8927)
                     /locus_tag="PK125_00815"
     CDS             complement(8724..8927)
                     /locus_tag="PK125_00815"
                     /inference="COORDINATES: protein motif:HMM:NF012607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB36679.1"
                     /translation="MRKTTKATKDIIGKGTKLKIIGRAGIGVDNVDVTASAEQGIVVM
                     NTPQRNALAVAEHSVTLVMFAGV"
     gene            complement(9075..10214)
                     /locus_tag="PK125_00820"
     CDS             complement(9075..10214)
                     /locus_tag="PK125_00820"
                     /inference="COORDINATES: protein motif:HMM:NF012488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HPB36680.1"
                     /translation="MQKRYLLAPGPTQIPPEVLLKMAEPILHHRNPMFEEIVAEVRED
                     LKWLFGTKNEVLFFASSGTGAMEGAVTNMLSPNDKAIVVRSGKFGDRWANICKAYGIQ
                     TVEINVPWGDEIDPALVEKALKENPDTKAVYTHATETSTGVRYPVREIATIVKNYPNT
                     ICVIDGITGVGVFELPMDEWGIDILVAGSQKAFMLPPGLAFAGVSDKAWAMNKTSKMP
                     RFYFNWTKELTNLQKNQTNFTPAISLIVGLRESLRMMKEEGLEKIYKRAEILARATRE
                     GAIALGLKPFAKNPSPAVTAIYAPEGIDGQAIYKSLWKKYGVTGAGGQDHLKGKIFRI
                     ATLGYADKYDVVTAMAALEFTLKDLGHKFKMGSGVGRALEVLQEL"
     gene            10362..10745
                     /gene="nifU"
                     /locus_tag="PK125_00825"
     CDS             10362..10745
                     /gene="nifU"
                     /locus_tag="PK125_00825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008678265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold protein NifU"
                     /protein_id="HPB36681.1"
                     /translation="MAKGPYNEIVMDHFMNPRNVGEIEDANAVGEAGSGQCGDSMTLY
                     LKIEDDKIQDAKFMTFGCGAAIASSSMMTELLKGRSIDDALNLTNSEVLKALGGLPEA
                     KIHCSIMAEEAVRSAIEDYRKRKGV"
     gene            10761..11144
                     /locus_tag="PK125_00830"
     CDS             10761..11144
                     /locus_tag="PK125_00830"
                     /inference="COORDINATES: protein motif:HMM:TIGR00149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HPB36682.1"
                     /translation="MFEIRVKTSKRVESRDITDEIISAVQGKSGRILHIYTPHTTCGI
                     VINEDADPHVMEDIMEALDRMVPRNYPYTHMEGNADAHIKSILTGCSVTVPFSDEAPA
                     LGTWQGIFLMEFDGPRERKVFITLV"
     gene            11188..11652
                     /locus_tag="PK125_00835"
     CDS             11188..11652
                     /locus_tag="PK125_00835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB36683.1"
                     /translation="MKHVLRLTSKTFWVISVCLLIIMPLYAKDDFTKPKELGVYVKTD
                     KGLKRLVPNIVFNEKGVLFVEPNNPSHFLLKDIQFFVIHGKYDTKVLTLNPLLFFQPS
                     PLGKQRYIFGKDIAFDLKKKSDDLYVVKPKELLGRGYIALWINDSAWDFIIE"
     gene            complement(11642..13516)
                     /locus_tag="PK125_00840"
     CDS             complement(11642..13516)
                     /locus_tag="PK125_00840"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HPB36684.1"
                     /translation="MITVSSLSKSYGSQVIFDRISFTIGAGERVGLVGRNGSGKTTLF
                     RLILGDEAHDGGTITIPRHYKIRHLSQHIRFKNSTVLGEASRELPIHEDGIDQSYKSK
                     KILIGLGFSTDEFGRNPHELSGGFQVRLNLARVLLSEPNLLLLDEPTNYLDIVSIRWL
                     TQTLRDWKGEFILITHDREFMDNVTTHTMGIHRSKIRKIAGSTHKLYDQILQDEEVYE
                     QTRINEERKRKDAEGFISRFRAQANRARAVQSRIKAIEKMGKLEKMPEANNLDFQFSS
                     APFQGKWLLEAENMSFSFQSDSPRLIKELSFAIKKKDRIGVIGKNGKGKTTLLRLLAG
                     NLMPVEGRVIVHKNVELAYFGQSNIDLLDPARTVEEEILGVLQDYNKNAARNICGAMM
                     FEGDNAMKKVAVLSGGEKSRVLLGKLLARPSNLILLDEPTNHLDMESIDSLVEAIDNF
                     PGAVVIVTHSEMILNAVAKRLIVFDDGKTSVFQGTYRDFLERVGWEDEDKNMVFERTA
                     EEERERPSIKKKEQKRLRAEYIQVRSKALGPLEQKMASVEREIMDLEKKIGDYNQTLI
                     RATTLGDGKSIASLSITIHNMRKEIDQLFEELDRFTKEHIARSKDLEKKWGEITSTIQ
                     "
     gene            13873..15957
                     /gene="nrdD"
                     /locus_tag="PK125_00845"
     CDS             13873..15957
                     /gene="nrdD"
                     /locus_tag="PK125_00845"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR02487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HPB36685.1"
                     /translation="METTDLNLFVRTSEETISDWDRSKIADALIRETLIDQDTADQIS
                     EETELMIRRSGVRVVTAPLIRELVNAKLIERGLEGARKMHTRLGMPVFDVDSLILHPN
                     KENANVPHGPEATNLTLAERIKKEYALLCVFSEDVADAHMNGDIHLHDLGFIDRPYCS
                     GQSLEYIKKFGLDLPHSLSMAKPAKHPEVLLAHLVKFAAALQSNFAGAIGWDAVNIFF
                     APYLEGLSDDEMKQFAQMLIFEFSQQAVARGGQAIFSDINLYWEVPKHFENVSAIGPG
                     GQFTGKKYGDYEKESQRFAWKLFEVYKEGDGAGRPFFFPKPLVHITEKFFRTEGHMDF
                     LNHIADVSADKGNTYYVFDRGETAKISECCRLSFKLEKSDLRDAMEPWRMRYCALQNV
                     SLNLPRIAYTAQHDDTKLMDLITQRFLLATKAHKEKRAFLEKLLAHGEDGPLALLTMN
                     RDGMPYLRFDRAAHLIGMVGLNEMVQIHTGEEMHESKQALKFGLKIMAHMKLLTDKIQ
                     RQEGFKIVLEQTPAESTSYRFARLDLHYFSPSSGRVVKGDIASGEVYYTNSTHLNVGY
                     PMNPIERVTNEGIFHPLIEAGSISHVWLGESHPYKQALADFVIKTFRHTTNDQIAFSP
                     EFTTCNKCHRTSRGLDDICSYCGSSDVDGITRITGYFTKVSSWNKGKLGELKDRHRVG
                     EYFNEQQKVANG"
     gene            16059..16322
                     /locus_tag="PK125_00850"
     CDS             16059..16322
                     /locus_tag="PK125_00850"
                     /inference="COORDINATES: protein motif:HMM:NF024590.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HPB36686.1"
                     /translation="MGIVKIFYKDDCPMCPVAKKLGEHLAAENIGVQHYNVETADGLA
                     EATFYHVMALPTILVEDEDENGIGEWRGSVPRIDEVLNAFRNA"
     gene            16303..17073
                     /locus_tag="PK125_00855"
     CDS             16303..17073
                     /locus_tag="PK125_00855"
                     /inference="COORDINATES: protein motif:HMM:TIGR02495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0051353 - positive regulation of
                     oxidoreductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HPB36687.1"
                     /translation="MPSGMHKFPIKGFIETSFLDWKGELSSVIFFAGCNFRCPFCHNS
                     DLVLRWDTLSDVHLEYIILTLRKYKQWVNKVVVTGGEPTIHMGLYNVLGYLKREDIQV
                     KLDTNGSSPSVLRGLVAEGLVDYIAMDVKGPLERYDTWCGTKVDIKKIEESIRFIMEG
                     NVDYEFRMTVVPSLHREEDVYQVAEYLRASDKFFLQEFKPRQCIDRSFLNVQPFPKDV
                     FERMDKNVQQIRVTGIKPEERIIKFREKILKPDRADSK"
     gene            complement(17094..17798)
                     /gene="rpoD"
                     /locus_tag="PK125_00860"
                     /pseudo
     CDS             complement(17094..17798)
                     /gene="rpoD"
                     /locus_tag="PK125_00860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176834.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
     gene            complement(18270..18608)
                     /locus_tag="PK125_00865"
                     /pseudo
     CDS             complement(18270..18608)
                     /locus_tag="PK125_00865"
                     /inference="COORDINATES: protein motif:HMM:NF015912.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor region1.1
                     domain-containing protein"
     gene            18807..19253
                     /locus_tag="PK125_00870"
     CDS             18807..19253
                     /locus_tag="PK125_00870"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HPB36688.1"
                     /translation="MFKKILCPVDFSEFTDEILSYAEIIAKRFDSELHLIHVIPNLSY
                     FTPYESFLTPENLVAIERNIEGEVGKDFDKITKKLDFPFKRIIKTGVTSVEIIDYIKQ
                     QGIDLVVMGTHGRSGIEHILIGSVAEKVVRKSPCPVLTVRPKSKTF"
     gene            complement(19341..19763)
                     /locus_tag="PK125_00875"
     CDS             complement(19341..19763)
                     /locus_tag="PK125_00875"
                     /inference="COORDINATES: protein motif:HMM:TIGR00738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HPB36689.1"
                     /translation="MKINTQIRYGVRALCYISFNSNGNPIQVKHISEHEGISPRYIEQ
                     IFQKFKKAGIVKSIRGPCGGYYLAKDPSEIRIGDVIRATEGGDFKLVFCDGLKKNAAK
                     TCERLNYCIVQESWEEGSRKLMAYFDSISINDLCKKPE"
     gene            complement(19805..21139)
                     /gene="radA"
                     /locus_tag="PK125_00880"
     CDS             complement(19805..21139)
                     /gene="radA"
                     /locus_tag="PK125_00880"
                     /inference="COORDINATES: protein motif:HMM:TIGR00416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HPB36690.1"
                     /translation="MAKKTVFSCEACGHETFKWMGKCPKCGGWDTIKEFRIEKEELSQ
                     QEKPIFVSEEDLPEERIILGINEMDRVLGGGITCGSSILLGGDPGIGKTTLCFEIASK
                     MVELGFDALYVSGEESLRQLSSRKKRLHLKGSFPILATNHVEDILRAVSDKHYKFAVI
                     DSIQSTYNSRLPMLPGSVSQIKDVSTRLIMEMKRSETAHVFIGHVTKEGAIAGPKILE
                     HMVDTVLYFEGDKTLPYRMLRAIKNRYGPIDEVGIFQMRTEGLVSVENPSEFFVADRG
                     DISSGSTLFPYVTGSRPIVLEVQAITPKTTFSMPKRLSLGYDINRLFILIAVLEKVLE
                     KPFFDRDIYINVTGGMKVADPAIDLAVAGSILSSYNDMNIGKDTALFGEIGLTGEIRK
                     VINMEARLKECERLGIKRVLCPRGAERIPGVETIPLKTIRDLHKQLVEKNDA"
     gene            complement(21132..21932)
                     /locus_tag="PK125_00885"
     CDS             complement(21132..21932)
                     /locus_tag="PK125_00885"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HPB36691.1"
                     /translation="MKILFSTGCLYYLPTKDVFLLAKEAGFDGCELVIDRRFNDSRSI
                     DTALQCREILPILSIHAPFMKMATWGNQVCSLQRSVEVAKTLDAEIVNFHPPNWFSME
                     VAFFRWFRSVQDFQSKLGADRVILAIENMPRIGKRVLLAPYILNSFKDLIEFGVSKNL
                     YFTFDTTHLATFGNDVIDAFLKFFKTKRLKNIHISDFGDYESHLFLGCGELPMVKLLN
                     TIGRLGYEGMVTLEISPHELPRTREWLVRMLKYQVAFMRRHLRKDFNG"
     gene            complement(21929..23155)
                     /locus_tag="PK125_00890"
     CDS             complement(21929..23155)
                     /locus_tag="PK125_00890"
                     /inference="COORDINATES: protein motif:HMM:NF012766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HPB36692.1"
                     /translation="MHLWRNARQSGKTSRFHTMKYVPREEIESRISKVKKLMEEASLD
                     GAFFHYKIDYYYLAGTMQDSLLFIPSDDEPILFVKREISRARRESPIKQITPFRSIKE
                     IFTHIKPMKRVGLQLDVIPYNDVIRVKEYLGDAEMVNVSPMIQEIRKVKSPFEIGLME
                     RAAEISKRVYKKIPELLREGMTEIELGGILEAYAKTHGHEGLLRVRSLNYEAYTWHIL
                     SGRTGSIVSQSDSPMGGLGLSPAFPVGASIKKIKKNEPILVDFGISYHGYHMDQTRMY
                     AIGSMPDLFIKAYEACKEIHYRVLDKIIEGAVSKDIFEFSKQLAEKMGYAEYYLGYGH
                     HKVRFLAHGIGIELAEYPYIAANHNYSINEGTVMAIEPKMVFPKKGSCGIENTVLIEK
                     GSYRVLTNTDEHIIIV"
     gene            complement(23112..23882)
                     /locus_tag="PK125_00895"
     CDS             complement(23112..23882)
                     /locus_tag="PK125_00895"
                     /inference="COORDINATES: protein motif:HMM:NF012596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HPB36693.1"
                     /translation="MAFNHITFEKKGKVATISLNTPPSNWLTISMMKEINEVLDDVKK
                     DASIQMLIFDHAGEKAFCDGVDVADHTEDKVDEMIDVFHRMFRHLAEMDLTTVALVNG
                     RSLGGGCELMAFCDIVVASEKARIGQPEIAVGVYPPVAAAWFPKIIGLQKTYELLLTG
                     KIISAKEAQAIGLVTAVLPVENFKAEVEKYLADFLNKSRPVAMWTKRAIKAGLSLDFL
                     QALKASEIIYNKGCMATEDAKEGINAFMEKRKAVWKDK"
     gene            complement(23979..25127)
                     /gene="oah"
                     /locus_tag="PK125_00900"
     CDS             complement(23979..25127)
                     /gene="oah"
                     /locus_tag="PK125_00900"
                     /EC_number="3.7.1.21"
                     /inference="COORDINATES: protein motif:HMM:TIGR03200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0019439 - aromatic compound catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-oxocyclohex-1-ene-1-carbonyl-CoA hydratase"
                     /protein_id="HPB36694.1"
                     /translation="MGLEWMPREHEMKDHSRHGTQWWGTEAPCTVYEKRPLKDPKGNV
                     IPGLYVAWVTLNNPAQFNSYTTEMVKGVIAGFENSSTDRSVVATVFTGVGPYAFCTGG
                     NTKEYSEYYSMRPEEYGAYMELFNNMVDSILMCKKPVICRINGMRVAGGQEIGTACDI
                     SISSDLAILGQAGPRHGSAPVGGASDFLPWYLGVEDAMWSCVSCEMWSAYKMWWKGLI
                     SKAFPVLKDDKGNWVRNPQVITDAFVKDGEIVYGEMKTGQEFKDARAWVQDKQKKGEV
                     DFSLLDAEVDRIVWIFANLFPGCLMKSIDGVRQKKKFFWDQIKNDHRYWLATNMMGEA
                     FLGFGAFNTKKITGVDTIDFIKNRQLIAEGKLVDTAFMEQVLGKPQGK"
     gene            complement(25172..26281)
                     /gene="had"
                     /locus_tag="PK125_00905"
     CDS             complement(25172..26281)
                     /gene="had"
                     /locus_tag="PK125_00905"
                     /EC_number="1.1.1.368"
                     /inference="COORDINATES: protein motif:HMM:TIGR03201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0019439 - aromatic compound catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-hydroxycyclohex-1-ene-1-carbonyl-CoA
                     dehydrogenase"
                     /protein_id="HPB36695.1"
                     /translation="MADLPKTIKQWQMTQPTVYNRETKETTPGKLEMVEIPVPELKEG
                     EVLIEIAGCGVCHTDLGYFYDGVPTVSKPPLALGHEISGTVIAGDPAWVGKEVIIPAV
                     LPCRKCILCKTGRGNRCLAQKMPGNSMGVFGGFASHIVVPSIDLCLVADKKGYALEQL
                     AVVADAVTTPYQACKRADLQPGDNVIVIGATGGVGVYMAQTVKALGAKTVIGIARNPD
                     KLKRALDYGCDYTISTLEKDNKALVKEWRDYCKSRGLDNTGWKIFEVTGTKAGQDLAL
                     DLLSFVGKLLVVGFGLSRSEYMFSRLMAFDAEIIGTWGCLPEYYPIVLDMVLNKKIVI
                     DPFVEVRPMSTIAATFDEIHKAGSPAKRVSLKPDF"
     gene            complement(26334..27089)
                     /locus_tag="PK125_00910"
     CDS             complement(26334..27089)
                     /locus_tag="PK125_00910"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase family protein"
                     /protein_id="HPB36696.1"
                     /translation="MGKLEGKNAIVTGGGRGVGRAISLAFAQEGANVVVNYAGNQKAA
                     DEVVGMIQAMGRKAVAVKGDVGQEEDAKRIVDACVENFGSIHILVNNAGISKPAMLHK
                     LTVDVWDEVVNVQMRGPWLMIKAASKYFMEQKYGRIVNVTSVAGMVGTIGQINYAAAK
                     GGVVTLTKSAARELAKFNVTANVISLGIVTTEMTMTLQTDDKLKEIYVRRILLNRYAE
                     PEDVAPAFVFFASDDARYITGQVLPVDGGYGMT"
     gene            27479..28873
                     /locus_tag="PK125_00915"
     CDS             27479..28873
                     /locus_tag="PK125_00915"
                     /inference="COORDINATES: protein motif:HMM:NF024994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HPB36697.1"
                     /translation="MNQKLARLFSPHSVAVVGASNNFDKLGYHVMKSLVGNYRGKIYP
                     INPKDYKIWGLDSYASLGAISDNIDLAVIVVPAHMVPDALHLCGGKGVQGVVLITAGF
                     REIEDPGGSVREEEIREIAAHYNLPIIGPNTFGFVNLSSGVNASFTSEFSRIEKGGVA
                     LISQSGGICHLCGFLAIEQGVAMSKLMSLGNRCNVDFPELLSYLVEEDSTTRVIALYI
                     EGLDKPRRLLEVAESLRGKKPVIAYKAGKSEKGDSASRFHTGTLAGNHAIWKGALRQA
                     GILEVDTAEELLDTAKAIDACPLPRGTHIAILSSQAGPGMIASDALEPAGLKLCRFSE
                     VTQERIDQLLPPVAIRTNPVDMGPAWYNPKNIIEILKTVLGDRDTDGVIFLAMYASAN
                     LKLVEGMVEYMKSIESFQKPVIACFSAPPGVWDEPVKALDRKKGVVTLPTPERAVLAM
                     ANMWKVGFLMNRKR"
     gene            28878..29534
                     /locus_tag="PK125_00920"
     CDS             28878..29534
                     /locus_tag="PK125_00920"
                     /inference="COORDINATES: protein motif:HMM:NF024938.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HPB36698.1"
                     /translation="MESLLLGINALTFLEKEGFPLLKSYLAQNEKEAASRSLEIGYPV
                     TLKISSPDMIHKTEAGGIRVSLKDEIEVRHAFRELVDSFSSSYPEKWLDGIIVQKQGS
                     GIEVIVGMLKDLQFGPVLMFGLGGVFVEAMKDVSFRLIPINASDAKDMMEELKGYDIL
                     RRSRSGPIDLPAIEDFLLQFSRFIVLHPEVKEIDINPLFISSLGSGVQICDARIKVVK
                     "
     gene            complement(30446..>30630)
                     /locus_tag="PK125_00925"
     CDS             complement(30446..>30630)
                     /locus_tag="PK125_00925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB36699.1"
                     /translation="CHNSPDFACQGNTNFKRDTEAVVLHNAAFRCPRDYKFILQNRET
                     RDSEYPHCGQSVKSSG"
     assembly_gap    30631..30730
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(30747..32036)
                     /locus_tag="PK125_00930"
     CDS             complement(30747..32036)
                     /locus_tag="PK125_00930"
                     /inference="COORDINATES: protein motif:HMM:NF021387.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050043 - lactate racemase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactate racemase domain-containing protein"
                     /protein_id="HPB36700.1"
                     /translation="MKPRYYLCLGPNRKEFFSLSNRWNVVHFVEKEKKVFMPTIEEMT
                     ERALLQPIGSPSLDELTGRVCRVAIIIDDATRPTPVKRILNVLLPLLAKKGVTKEKIT
                     LVIALGTHMPVKHEDLELRIGSDLMSGYRIVQHNAWQDNLIPFVLDGNQIVKINPHVV
                     QADLKIGISSILPHPMAGYGGGPKIVMPGIADFDYTRDHHMRYTIHPQARAGLIKGNP
                     FHEKVLEVARTVGLSLTINCVYNQQGEPVQIIAGSPEAAFFKAVELCYEELGHRFQEK
                     VDITISSTYPHTHGHQFAKGFSAPDAVTKDTGAILLMAPLVTPVPDEFRDSFNVVREK
                     SENQPERFVRNAMAQGLPFLPEKPLEFNMAMSCMILRRPIRTISVSPMISREDASTMG
                     MEYASSLKQGLRVLEKAYPEATVAIFPSGGLVVPVTD"
     gene            complement(32241..32453)
                     /locus_tag="PK125_00935"
     CDS             complement(32241..32453)
                     /locus_tag="PK125_00935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB36701.1"
                     /translation="MRKYFSQATLKEIATLRNIGKITASTGVAYYCLNESPKAFSLWS
                     DPALYFVKNGGRNSMATEPDIFSMLP"
     assembly_gap    32536..32635
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(33246..33896)
                     /locus_tag="PK125_00940"
     CDS             complement(33246..33896)
                     /locus_tag="PK125_00940"
                     /inference="COORDINATES: protein motif:HMM:NF012451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HPB36702.1"
                     /translation="MCDREHFLDVLGLKPSASHEEIVNAYRVLMEYWNPDRVPDFYKQ
                     KAMEGRDKVEVAYQELMGLRKKDQKGAPPLTQKLPEQKKADQSITEKEGILLKKDKDG
                     TLVYLDKKSIAFNKDKIEISVNIYPPEGSTRFNTAHGYIRRAGYGSLECIAEKWGIGL
                     SNKVFIRYGQFYKSTCGRFVEATADIRKVWKPITPGTLEEIAWKVVDGILNREKMA"
     gene            complement(34081..35994)
                     /locus_tag="PK125_00945"
     CDS             complement(34081..35994)
                     /locus_tag="PK125_00945"
                     /inference="COORDINATES: protein motif:HMM:NF019325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP-like repeat-containing protein"
                     /protein_id="HPB36703.1"
                     /translation="MVDASVSAPVLKWQHGGCFSSWCETGWYSSPAVADLDGDGRMEV
                     IASAYSIVVLNGETGALKWRVKSGHDRNEPNAENVGRTWPGIIVADIDHDGELEIVTA
                     HGGGWVSVLSRDGYFKSRGWPWKNPGGQEFRSLSVGDLDGNGFLEIIVGRAAGSKTNT
                     WVLEHTGEIRQGWPRLKAGDESSAWGIYNANIGLGDLDGDGLPEIVIPSDTITVSAYK
                     SDGTQLSTNPMYHGNPGHDMDAWGEVPAYVDLEYEARGWGPCYDGFTARANFAQGPAN
                     VVDMNGDGVKEVVVVGYVHNCNTDPYTNLYNTPFIFNADRSRFNKDGFNWTTIPINTG
                     APLSDDYNVIESVQPNPVTVDLDGDGRAEILYPSSDGRMHCFWLDKTERGNWPYSVYN
                     LVEGFYRFASEPVVADLDNDGHAEVIFASWAQKGSNATGKLHILDYMGYVLHEVDLPY
                     AFGGVDWNGALAAPTLADIDGDGELEVVLNTVHSGFVAYDLPGTASARVLWGTGRGSH
                     LREGSNGEVPPVLLGPDLVGEWNQITLTGPNKKGLYKVKGSVTVSNRGNMPSNACLMG
                     VYHTDALHMMLKKPLKVKKLKPGFNIIKKFVTRGIALNAGDNLFTVSVDLENRVAETN
                     EENNKASRIVTIP"
     gene            36295..36981
                     /locus_tag="PK125_00950"
     CDS             36295..36981
                     /locus_tag="PK125_00950"
                     /inference="COORDINATES: protein motif:HMM:NF025729.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4412 domain-containing protein"
                     /protein_id="HPB36704.1"
                     /translation="MRFFKVKYSIIVSCIVGFVLSGILAFAAPPDNFTATIVSQGMEM
                     PMAKMGNKSRVENPAIKGVVTIVIADAKKTIMMNTNAKIYSEQVTQEQDQMTNMYDPD
                     VVFEKKKVGNETIDGHPCVKYDTVYYRKSKPNEKHKAIIWEAQDLKEFPIQMEVTVPA
                     NPKYPGSGGKMIVKYKDVKLGAATASMFEVPPGYKKVNSVQEVMGFGGMGNIEEMMKK
                     IPKGQRPPKQ"
     gene            complement(37035..37844)
                     /locus_tag="PK125_00955"
     CDS             complement(37035..37844)
                     /locus_tag="PK125_00955"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HPB36705.1"
                     /translation="MPFKKINGFNIYFEVHGEGDTIIFLHHGFGCSKIWNQIYPSFVA
                     DGFRIVMYDRRGFGQSEKGDGFYGFYDSDRYRPESVEELRLIKEWLGINSCYLIGQCE
                     GGVIGVDYAVKYPEEVKALTIASTQCYSEVPMTELNVKTLVNTFAELSPELQAKMVEW
                     HGEPAEKMYNQFAKQGGAYGKDYFDLRPILHMVLCPTLVLYPDRSSIFDVEQSIAFYR
                     HLTKGELAVFPRCGHNTYEQRTEDYINTILAFLKRIRDGLGAVKPAVSCLA"
     gene            complement(38150..38821)
                     /locus_tag="PK125_00960"
     CDS             complement(38150..38821)
                     /locus_tag="PK125_00960"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HPB36706.1"
                     /translation="MIKALIFDIDGTLIDSFEAYYTVFNEGIADYNVEKVSRRDLRDL
                     LAKGLSLRQILENVFTSPIDNVTYSMCREKILTLFRVAQLEKIKPFPGTEELFRYLMT
                     AGIKIGIATGRTSSAEDETLRFKKLGLYRYISAIVTSQEIEHRKPAPDAIIECAKRMG
                     IPIDKCIVVGDTESDIIAAKSAGAIAIAVTTGHEDEQTLLKARPEIVLKSMVELLSYL
                     KKRGL"
     gene            complement(39096..40190)
                     /locus_tag="PK125_00965"
     CDS             complement(39096..40190)
                     /locus_tag="PK125_00965"
                     /inference="COORDINATES: protein motif:HMM:NF014841.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HPB36707.1"
                     /translation="MNSRKESITAQGVYDIMHFEALGPEAQHLEEEIGKAKEQRQLPL
                     GHTYLITPSNLQEFLKENPGHPLPDIITTKTHSILPHDYLEGPKKSIITRSAGYDHFE
                     HLAETANIASLREYCVNAVAQTAMKFLYATAGELNHYTKNIETFERKNSNAFMELDKH
                     RIITVYGVGKIGRKIYELAEANGLTVQGVDIREEELNDIYERSVRFVTKEEAVISSDI
                     MVNAMNLTKKKESKFFNVGYFSREYLSMAKDGIIFINVTRGEIAPESVLLELYQSGKI
                     AGLGLDVFTNEAELAKVLLGDSLMEKEFLAAKTLVNMALERRGNVYVQPHQGFNSDIA
                     AKAKAVEAIKHVVAWYKNKGKCFDEQLPYY"
     gene            40411..41352
                     /locus_tag="PK125_00970"
     CDS             40411..41352
                     /locus_tag="PK125_00970"
                     /inference="COORDINATES: protein motif:HMM:NF012472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HPB36708.1"
                     /translation="MKEQNLHSSISHIILGTWAMGGNHWGTCDDADALRAIETAIDNG
                     INTIDTAPVYGDGHAEELVGKAIRGKREKIFLATKCGLDIYTNKYQRNLSPSYIERDL
                     AESLKRLGTDYIDLYQCHWPDPQTPIEETMSALMHFHKQGKIRYIGVSNYTLAELEAV
                     MRCTSLFSLQSHYSLLERTVESDVLPWCRKNDVRFLSYGSLGAGILTGKYVECKRFPS
                     GDARSFFYRFFKPRYWPRVCQLVDAMKEMALSRGVKPGVIALVWLLQQPAVTSVIVGA
                     RSAQQVQDNIPLGGVNLTDSDMRLLDHLSQKVYAKEQ"
BASE COUNT        11275 a         9444 c         8384 g        12146 t
ORIGIN      
        1 aacttggctt atccaggtac accatgatga aagcgagaaa acatcttagg aaacttggga
       61 tcatagagac agaactccac atggcgaatg gccatgcaac aacacactat aaggtgaact
      121 tcagaagatt ccggacggct tttcaagaat tcatagagag ccagaaagtc cataccacta
      181 acgaaaccga actggaacca ggcatatata ccgaaactga gcctatggat gttgatgatt
      241 ggcctggaga tgtatattca atggccgaag agatggaccc taaccagctc attactttag
      301 aagattactg cgcttcagcg tttcttggtt ctccggttaa acttatggtt tctctgttcc
      361 gccttaaaac aggcaaagac catccaaaac tcacactgtc tgaattcagt aagttagagg
      421 cagaacttcc gatctgttca gatggtaatg aaactgtgag aatcgatgat gacaaaatca
      481 taatagaaat gcttattgaa tacacacgga cagcgtatga cgatcctgct gcctatacct
      541 tccagcattt taacaaacca gaagtaaaaa cacctcttta caaaaaagtt atggagcaac
      601 gtaaagaatc catggaggca aacaatgatt aagaatgaga tcaaatccaa tagtggcata
      661 gggaaaaagg caaaaggaag aggtgaactc gtaagacacc gcagaggtga gaggttaacg
      721 ctgaaagagg cggtcctggc aaattgttac gaatgcatgg gcggatacac ggatggtctt
      781 tgtgactgtg aaatacccga ctgtccactc taccagttca tgccatacaa ggaaagtaaa
      841 cgtgaggggg ccgaacacca tgagtgagaa tcgaatcaaa gtagtaaagg aaatcggcag
      901 aaaggcaaac ggaaaatcta aattactgaa acatctgaga ggagaaaatc tcactatttg
      961 ggaagctgtt aaggctcact gttacgactg cacaaggtac tatacaaatc ctgaaccttg
     1021 ggactgtaaa aatcttgaat gtaccctcta cccttttcat ctctacaata aggagtacat
     1081 gaaaaaggcc agaggattgg attagaaaca taatccactg acggtttaaa ccgtcaatga
     1141 ttgtttccca tagaggctct acaggtcctt taagcacatt ggaatagcca ggagatgctt
     1201 tattgcccgg ttttttttca tctgctcata ggacctgcac aggcctcaac cgacatggtt
     1261 ggctgaagta aaacgatgct ttttcatttt tttgagaaac aaacggcatc tgaacttatt
     1321 tgaaatacaa acaaatacct tggggggtgt ataccccccc ccaaggtatt cacaggattt
     1381 attaggagaa aatatgaaca ttgattttca attatctgat caagacattg attccattgt
     1441 cgataagctt gaaaaacgac tgagacatct tttgtccaaa caatcctata cgtctgataa
     1501 tgaatatctg gatgttaaga gtctatctga atatctccat gtttccaaaa gttgggtata
     1561 tgagagaacc catctcaacg agataccctt cattaaactc cccagccagc agggtaaaag
     1621 gtcaaatggt acaacatctg gcaaggtact ctttaagagg agtgcaattg atgcttggct
     1681 tgaacggaat agtgtaccag aaattagcca atcaaaaaat ggcctaaatc tgcttaaagg
     1741 ggggaaggcc tcttgataat ttatcaagtt ccacggaaaa taggttttaa gatatcagta
     1801 tgttggatgg tactgtgcac caaattgtgc acttcattct tttacttgac ttctaacaat
     1861 gttagatgta taaagtatgt tatgcgttcg tggacaccag aaagaatcaa gactttgagg
     1921 aaacaattta agctatccca gacagccctg ggtaaactaa ctggtgttgc cgggaactac
     1981 atctacatgt tagaaggagg tgatagaact ccatcaaaaa ctttgtgttt acttttggat
     2041 cgtattgaag cagaactaca aggacaaaac aaaaaggaag gtgaacagta actatggcta
     2101 aaacaaaagg aatttacaaa cgaggcaaca tctattggat tcggtatgct ggccctgatg
     2161 gtaggatcat cagagaatct acgggttcgt ctaaaataaa agatgccgaa tcaaaactga
     2221 ttgatcgtag gaaggaagtt aaggaaggag agctacctga gatacacaag attgaaaacc
     2281 atattttcag ggaacttgcc gatgcctatc ttgtctgggc tgaaaggcaa agatgtttca
     2341 atggcaaaaa atatcttata aaacagttag ttgacaagtt tggcaacctt cctctcagaa
     2401 gatttaacac gatgcttgtg gagcagttcc agactgaaag gatgcagaag ggaaacaaac
     2461 ctgcaacgat taaccggttc ttggcaaccc tgaagcatat gttcaccaaa gcagtcgatt
     2521 ggaacatggt tgaagagagt gtgctgaaag ggataagaaa agtaaagctt ctgcaagaaa
     2581 acaataagag gctcagattc ctttcaaaag aagaatgcca actacttctc aattcatgtg
     2641 acaatcattt aaagcccatt gtttttacag ctttacacac tggaatgaga aaggcagaga
     2701 ttctaaatct ctgttggaat caagtagatt tgaagcatgg atttatactg ctcgaaataa
     2761 ctaaaaatgg tgagaggaga gaagttccta tcaacgagcc gttacaggaa atgtttcaga
     2821 ggcagacaag acgattggat gtaccatttg tattctatga caacaggaat ggtcaaccat
     2881 atcaagacat taaacattct tttgcctcgg cttgcagaag agcaggtatc aaggactttc
     2941 atttccacga cttaaggcac acttttgcca gtcaccttgt aatggctgga gtcgatttgg
     3001 caacagttaa ggagcttctt gggcataaaa ccctcacaat gactttaaga tatgctcacc
     3061 ttgctccttc acataaagtg aaagcagtag atatccttgc tgaaagactc acacaagaat
     3121 ctactgcaca aaaagtgcac aatttagaga ttgttcagaa tggctgatgg ttgtaactgc
     3181 ttgaaataac tggtggagct gaagggggtc gaaccctcga cctcatgact gccagtcatg
     3241 cgctctccca actgagctac agccccactg ctttttttat atcaataaac ctttgaattg
     3301 tcaatgggtt ttgaacgcat gttcagctgt aactttcaat tttcgggagc cgagaatttc
     3361 cagtgcgtag ttcagaatat gacgaactaa cgaaacggct ggttgtcaaa tttatagaca
     3421 caagaggatt gctgaataac tatcgaccct gtcaaaaaaa gatggcagtc ttgcagtaat
     3481 tgtgatggct attccaataa caaagaataa aacttattta atataacaaa agaaatcaag
     3541 aagtaagtgc ttaatctgcc ttcttgtcaa cagaaactgc acacggtcct ttgcctgtgc
     3601 aagtgacccg agtgacaact gttttccttg gggacttagt ttccatttca gtcttcttct
     3661 tcacaagtgc ttttgctttc gttgttcccc tcagtttcgt gatcttaata ccatcttttg
     3721 tatactcaac agccactgta tctccaacga ctacatcgct caagtctttg tagcccttta
     3781 gtaccgggtt actggtatca aaggttacta cctctccctt tttactcaag acatctatca
     3841 agttcgaata aaccattgtt acagttccca caaaccctga accctttgtt tctgctatta
     3901 tttttgttag tgctatcctt ttggggctct tctttaccct ttcagtcttc ttcttcacaa
     3961 gtgcttttgc tttcgttgtt cccctcagtt tcgtgatctt aataccatct tttgtatact
     4021 caacagccac tgtatctcca acgactacat cgctcaagtc tttgtagccc tttagtaccg
     4081 ggttactggt atcaaaggtt actacctctt ttttatcctt cacctgcatc aggttttctt
     4141 caataagcgt tgctgttcct acaaaccctg aaattttcgg ttccgtcata atctttttca
     4201 gagttgctgt gttttctgaa gtgcctggcg cctgtggtgc tggtactttc tcggtcacta
     4261 aattttcttt gcttgatttt tcaggaggca ctttggagaa tgcaaaacct gtggaggcaa
     4321 aaatgatact gattagcacg gcgacagtta tttttatgtg tttcttcacg aactctacct
     4381 ccctgttctt gctatcagga aaaatcaaca tttccgcttt attaatcgcc accgccctgc
     4441 ttctttcctg tataaccaga atgattgata tacctcagtg ggcgcaaaac cagacaactt
     4501 ttgtgaattg ggcagaaccg tttgattatt ttagtcttat tggtgacacg gtctggcaac
     4561 acccttcctg taaatgttac ccctgcttgc gacctaccgt atttgtcata gtgtatcgct
     4621 gggggtggaa acttgtcaag tggttttttt gggcgttgct tgtgtataaa tcttccaagc
     4681 gcttcctaac cattcatgtt aattatttga tatcagagga tatgagctca ttacaatctc
     4741 agcagctttc ccaccacagg aatgaaatga tccagaaaca ggtctcgacg ttacaatctg
     4801 acaatgcata gactcataat aaagatgtct tgcatttgtg aaactgggca aacactggta
     4861 tgttgtcagg tcactcaaaa agacaatgcc aagaaagccg cgttatagtt aaatccatta
     4921 taatccagca gttacgggat tgtattgagt gattggatgg ctatttagtg caaatgtaac
     4981 gcttaaaaat cataatcaga aaaactattc aatttttgat gttattgacg aatagtgtcg
     5041 tctcttcgac gttttgtatg gttatcatca tctatctacc tcattcaaaa tcagtctccc
     5101 tccttatcaa tcgaataacg tatttaatgc tcgattttag tccatatacc gtttcaaaaa
     5161 gttgtcaacc tgtctgtttt tttgaagttt taaacaaaaa ttgtggaaaa cctgtgtaca
     5221 acatgaggat gtgaagcatg tgcttcctga tattattacc atatttctaa tatgaataga
     5281 ataaatgcgc aacaaattca tcaataatta cgatataatt ctgcactata atcatggata
     5341 tttccgtgta caattacaaa atacgatctt ttccatcaac attttcagct ctttatatag
     5401 agtaataaac ggtttgtgca aaggcttgat tttatgcact cttgcaaatg cagacatcca
     5461 ctgcctcacg tccctattac ctgttggtgg aactcccacg atatgaagtt tatcgcgaat
     5521 ttttgccatg ccttattcgg tacagaaaaa tggtatgaag gtgtgttaac ttttgcttcc
     5581 atccgcaaca cctcactcta ttgcatctta aaacgatttc aaaagatttt ttttgatttt
     5641 acccgatgct gtttttggaa gttttttaat aaactcaata tgttgtggga ttttgtatcg
     5701 ggcaagctta tcttcaagaa atgaacggat ctcgtcagcc gagattgaac aaccttcttt
     5761 gggcacgata aatgccttgc caacttcccc ccatttctta tcggttacac caatcactgc
     5821 cgcatcaagc accgacgggt gactgaaaag agccttttcc acttctgcag ggtaaacatt
     5881 ctctccgcca cttatgaaca tgtccttttt tctatcaaca atgtacgcaa aaccatcctc
     5941 atcaaatctc gcaagatcac cagtttgaag ccaacccccc ctcatgactt gtcttgtcag
     6001 atcaggcctt ttccagtaac cattcatcac tatgggacct ttgacaacaa tttctccaat
     6061 ttcccctgcc ttgactggtt tgcctttgcc atccactatc tttacctccc cgtggaaagc
     6121 aggttggccc acagagccca ttttctcaat ggctctacct gcaggaagcc agaagatagt
     6181 tgatacctct gtaagaccat agatctgaga aataatgatc cccttctcgg cataggcttt
     6241 gagcagtgca acgggtattc gttccccgcc cgtgaaaaag catttgacac tcgataaatc
     6301 gaattttgat atatcgcact cattaagaat tatgttgtag accgttgccg gtaactcaag
     6361 gactgtaact ctctcatttt ccaccgtttc cagtatttct tgggggctga atctcttcct
     6421 tacaataact gttcctccac ggtaaagaat gggagcaagt tcaactatga gtccccctga
     6481 atgaaacatg ggccttgcaa ccataacaat atcatgactt gtaagatcaa aatagattcc
     6541 agcgttcagg acattaaaga aggttttctg atgagagagg atagcccctt tgggcagccc
     6601 tgttgtaccg gatgtataca taatgatgtg tggatcttgt gccccggatt cccagggtat
     6661 gtttggctct gaatcagggt aggtcatgat tgagctttca tagtcaatgg cccaggttgg
     6721 cacttcacaa actcttttat tctccccgga agtgcaaaat atttccttaa acactgggag
     6781 tttctcgcgg agcatggcag cgtttcccat aaactcctca tcgaaaatga tgaatcgagg
     6841 actgctatcg cgcacgataa actcggtctc gggtaatgca agacgccaat tcaggggaac
     6901 aaggataccc ccagtctttg aaagtgcaag aaaaatttcg atatattgcc tacagttacg
     6961 aagtaacact gcaacacggt cacccttctc catccccata tggagaagaa aattggaaag
     7021 acgattaata cgctggttta gattacggta tgtataaggt acgccctctg aaatgagggc
     7081 tatctttttc ggctgtattc tcgcccactt ctctatccaa caaccaatat ttagcaccat
     7141 tttctcaatc acttttctcg ctccgctgaa aaaaatggat atatccgatc ttcgatcgta
     7201 tgcgtcaact acttatctat ctttatccag aacattcagg ataccacgca catcattgag
     7261 tatataagtc gccccaacat cttcgaatgt ctccctcgta atcctaccag tgagaacagc
     7321 agcgcttttt gccgaaactg caaggccaga ctggatgtct gtcgggtgat ctcctacaaa
     7381 aagtgcctct tcaggccgga ctccgatcaa ccgcaggact tcacaggcat ggtctggatg
     7441 gggtttgact ttctttacat catctcgggt tactatggct tcaacgaaat cccctatatc
     7501 agggaagacc tgaagcacta catcgagatg ggtacgagta aagattccca ctctaatacc
     7561 tttatctttc aattttgtca gcacttctct ggtatatggg aagacgtcct taccctctga
     7621 ggccatcaac tcaagtctcc tcaactcttc aaaggcctct cgtttaaaat ctggaaccaa
     7681 gccgccgagt ttttcttcaa caccatttat catttcaagg atatactgtc cctcttgggc
     7741 tttaatctca tcatcaccca catatcttcg tgctactttt tcaatctcac cacggacaag
     7801 tgggaaatct atggttaatt ccgtgagtgt tccatcaaag tcaaaaatta caccctttat
     7861 cataatatcc tttatttatt aaaccatata atatgctttc aatgctataa cctacctttg
     7921 taaacaactc tccttttcgg cagttctatc tttttgatcg ctaaaatgtt tgtcaaagac
     7981 tgaatctccc ttattacgtt ctcctatgcg tgcttatcaa ggtccaataa gtaaattgcc
     8041 agcccagcca cgttttcacg gtcaaagtgc atacctctta tgttgattcc cctgatataa
     8101 aatacatttg ctctgctggc aataacacct tatgtatcat aattgtaaat aaaaagcatg
     8161 tgtcctctga ggtcagtctt aaaatagatg ttgttcacct tatcttgtgc ttcttttgtg
     8221 gatgcttcag gatggggcat acagattgtt ttttgtgata aaacaaggag gttctctttt
     8281 agagagggat tttcatcaaa ggcatcaagg gaagcaaatc caacctggcc agaatcgagg
     8341 atatccaata aacctttctc tacaaaaata gaccctctcg ctacattaat aatagtcacg
     8401 cttttttatt tcggctgtaa gctgttttat tgatcatgcc ctttgtttct ttgactaatg
     8461 ggacatgttt tgtaacaaaa tcgctttctt cactggtgta tcgaaatcta ccagtttaac
     8521 gccaatgttt tgggcaaatt cctctgaaat ataagggcgc tatgcaataa cacgcatccc
     8581 aagggtatct gttttttcgg tcacaatggt gccaatatta ccaattccta taacccccag
     8641 ggtttttcgt taagctccat gcctgtgaga ggtttctttt cccacatatc ttctttcatg
     8701 gtcgtatctg cctgagagat ctttcatacc ccggcaaaca tgacgagggt aacagagtgc
     8761 tctgcaacgg caagggcatt cctctggggc gtattcataa ctacgatacc ctgttccgcc
     8821 gatgcagtta catcaacatt gtcaacacct atccctgccc taccgatgat ctttagtttt
     8881 gttcccttgc caataatgtc cttcgttgct ttggtggtct ttctcacaat cagcacatct
     8941 taatcgcgta taatggcctg tagttcttct gacctcaggc ctgtctttgt atccacgatc
     9001 agattggcag actttcaaaa tttcgactgt catatctgaa agagcatccg cgattaaaac
     9061 caggtgctat ccccttacag ttcctgcaat acttcgagcg ctctgcccac acctgacccc
     9121 attttgaact tatgtccaag atccttcagg gtaaactcaa gcgcagccat agctgtaacc
     9181 acatcatatt tatcagcata gccaagggta gctatcctaa agattttgcc tttcagatga
     9241 tcctgaccgc ctgcaccggt aacaccatat tttttccaca atgacttgta gattgcttgt
     9301 ccgtcaattc cctcaggggc ataaatagct gttactgctg gacttgggtt cttggcaaag
     9361 ggttttaacc caagggcaat agccccttca cgggtagctc ttgcgagtat ttctgctctt
     9421 ttgtatatct tttcaagtcc ctcttccttc atcattctga gggactccct tagccctaca
     9481 ataagggaga tggcaggcgt gaaattagtc tggtttttct ggagatttgt cagttctttt
     9541 gtccaattga aatagaacct gggcattttc gaggtcttgt tcatggccca agccttgtcg
     9601 ctcacgccgg cgaaggcaag acccgggggc aacataaatg ccttttggga acctgcaacg
     9661 aggatgtcta tcccccactc atccatggga agttcaaaaa caccaacccc ggtaatacca
     9721 tctataacgc agattgtatt gggataattc ttgacaatag ttgcaatttc tctaacaggg
     9781 taacgaacgc ctgtagaggt ttctgttgca tgggtataca cggccttagt gtcaggattt
     9841 tcctttaggg ctttctcaac gagcgctgga tctatctcat caccccacgg aacatttatc
     9901 tcaactgtct gtattccata ggctttgcaa atatttgccc accggtcacc aaacttacca
     9961 cttctcacga caatagcctt atcgtttggt gaaagcatgt tagtaacagc gccttccatc
    10021 gcacccgtac ctgatgaagc aaaaaacaag acctcattct tcgtgccaaa aagccatttc
    10081 aagtcttctc tcacttcggc tacaatttct tcaaacatag gattcctatg gtgtaaaatt
    10141 ggttctgcca tcttaagcag cacttctggt ggaatctgtg ttggacctgg agcaagcaag
    10201 tatcttttct gcattgtatt cctccttatt gataaatgta cattttttat caaatgagcg
    10261 ttaaaaatac aagaaaaatg ataaaatcac aaatacatga aaaaatgctc tggacatggt
    10321 ataatgcaat catataaacc tactgatagt gaggtatcgt gatggctaaa ggcccctata
    10381 atgagattgt gatggatcat tttatgaacc cacgaaacgt tggagagatt gaagatgcta
    10441 atgccgtagg agaggctggt agtggccaat gcggtgactc catgacgctt tatctaaaaa
    10501 tagaggatga caaaatccaa gacgcgaaat ttatgacctt tggttgtggt gcagcgattg
    10561 cgtcgagcag catgatgaca gaacttctga agggaagaag cattgatgat gcccttaatc
    10621 tgacgaatag tgaagtgtta aaagctttgg ggggattacc tgaggctaag atacactgtt
    10681 caattatggc agaggaagcg gtcaggtcag ccattgaaga ttaccgaaaa agaaagggtg
    10741 tataaacgga cgctaatgca atgttcgaga tacgagtcaa gacctccaaa agagtggagt
    10801 caagagacat cactgatgaa attataagcg ctgttcaggg aaaatctggc agaattctcc
    10861 atatctacac tcctcacaca acatgcggaa ttgttatcaa tgaagatgcc gacccccatg
    10921 tgatggagga tattatggaa gcccttgacc gaatggtgcc tcgtaactat ccctacacac
    10981 atatggaagg aaatgctgat gcccatatta aatcgatcct tactggttgt tccgtaacag
    11041 tgcctttttc tgatgaggca cctgcattag gtacttggca gggtattttc ctcatggaat
    11101 tcgatggtcc aagagaaaga aaggtcttta ttacccttgt ttgaataaag gggttcagcc
    11161 aatgcgtaaa atctctggag gttgtgaatg aaacatgtct tgaggctcac atctaaaact
    11221 ttttgggtaa tatctgtttg tctgcttatc atcatgccat tatatgccaa ggatgatttt
    11281 acaaaaccga aggaattagg tgtctatgtc aaaactgata aaggcttgaa aagacttgta
    11341 ccaaatatcg tttttaatga aaaaggcgtt ctttttgttg aaccaaacaa tccttctcac
    11401 tttcttttaa aagatatcca gttttttgtc attcatggta agtatgatac gaaagtcctt
    11461 accttgaacc ccttgttgtt ctttcagccg tcccccctcg gcaaacaacg ttatatcttc
    11521 ggcaaagaca ttgccttcga tctcaaaaaa aagtcagatg acctgtacgt ggtaaaaccg
    11581 aaagaactgt taggtagggg ttatattgcc ctttggatca atgattcagc gtgggatttt
    11641 attattgaat agttgaggta atctcacccc actttttctc caaatctttt gacctggcaa
    11701 tatgttcttt ggtaaaacga tcaagctctt caaaaagttg gtcaatctcc tttcgcatat
    11761 tatgaatggt tatcgataat gatgctatcg atttcccgtc acccaacgtt gtggcccgaa
    11821 tcagggtttg gttgtaatcc ccgattttct tttcaagatc cataatttcc ctctcaactg
    11881 aggccatctt ctgttctaga gggcctaggg ctttagatct gacctgtatg tattctgccc
    11941 gcagtctttt ttgctctttc ttttttatac ttggcctttc tcgttcttct tcagcggttc
    12001 tttcaaaaac catgttctta tcttcatcct cccacccaac ccgttcgaga aaatccctgt
    12061 atgttccttg gaaaactgat gttttcccat catcgaatac tatcaacctt tttgcaacag
    12121 cattcagaat catttcacta tgagttacaa taacaaccgc acctggaaag ttatctattg
    12181 cttcgactag ggaatctatt gattccatat cgaggtggtt ggtaggctca tcaagaagga
    12241 taaggtttga aggccttgca agcagttttc ctagcaacac cctgcttttt tctccgcctg
    12301 agaggactgc tacttttttc atggcattgt cgccctcaaa catcatcgcg ccacagatat
    12361 ttcgcgctgc attcttgttg taatcctgta gaacacccag gatttcctct tctacagttc
    12421 ttgccggatc aagaaggtct atattactct gaccaaaata agcaagctct acattcttat
    12481 ggacaataac acggccttca acaggcataa ggttgccagc aaggagtcta aggagcgtgg
    12541 ttttaccttt tccatttttt ccaatcacac caattctgtc ttttttcttg atggcaaaac
    12601 ttaactcctt aataaggcgt ggactatccg attgaaagga aaaagacata ttttcagctt
    12661 caagaagcca ttttccctgg aatggtgctg aactgaattg gaaatcaagg ttattagctt
    12721 cgggcatttt ctcgagcttg cccatcttct ctatggcttt gatgcgcgac tgaacagccc
    12781 gggcacgatt tgcctgggcc ctgaaacgac ttatgaaccc ttcggcatcc ttacgctttc
    12841 tttcctcatt gattcgcgtc tgttcatata cctcttcatc ttgaaggatt tggtcataaa
    12901 gtttgtgggt agagccagct attttcctga tttttgatct gtggatcccc attgtgtgtg
    12961 tcgtgacatt atccataaat tctctatcat gggtaatgag gataaattcg cctttccaat
    13021 cacggagggt ctgggtcaac caccttatag atacaatatc aagataattt gtcggttcat
    13081 cgaggagaag aaggtttggt tccgagagaa gtactctcgc aaggttgagg cgtacctgaa
    13141 aacctccact caattcatgg ggattccgcc caaattcatc agttgaaaaa cccagcccga
    13201 tcagtatttt ttttgacttg tatgattgat ctataccgtc ttcatgtata ggaagttccc
    13261 tgcttgcctc accaagaacg gttgaatttt tgaaacggat atgttgtgac aggtggcgga
    13321 ttttataatg gcgtggaatg gttattgttc ccccgtcatg agcctcatct cccagaatca
    13381 accgaaaaag agtggtcttc cctgaaccat ttcgacccac aaggccaacg cgctcacctg
    13441 caccaattgt aaaactaatc ctatcaaaaa tgacttggct gccgtaactt ttgctgagac
    13501 tgcttactgt gatcatattt gtatatagta tatcgtatgt agcattttac gaacaagaag
    13561 tagaaaccaa tatactacat gggaaagaca gaacgaggat tatccatatg caacatacaa
    13621 acgatggtat acaagctgtt atatatttgt atacatttta agcccataag atacactttc
    13681 accagcatat aaatattttt taagaattct cttgacaacg ttttttacta gatatagtat
    13741 tgatgtttag ttagcctact aaatataggg gtgtgtaatc attaaggggt cattattgcg
    13801 aacaatttat tgtttatgcc caaccttctt gaagcgcgcc cgccattcca ttctattgaa
    13861 aaggggggat taatggagac tactgattta aatcttttcg taagaacctc tgaagaaaca
    13921 atatctgact gggacaggtc aaaaattgcc gatgcattga tccgggaaac actcattgat
    13981 caggacactg cagaccagat cagcgaggag acagagctga tgatcagaag atcaggggtc
    14041 agagttgtta cagcccctct catcagggaa cttgttaacg caaagctgat tgaaagaggc
    14101 cttgaaggtg ctcgaaagat gcacacaagg ctcggtatgc ctgttttcga tgtggatagt
    14161 ctcatcctcc atccgaacaa agaaaacgcc aatgttcctc atggccctga ggcaacaaac
    14221 ctcaccctcg cagaaaggat caagaaagaa tatgcacttc tctgtgtttt ctctgaagat
    14281 gttgcagacg cacacatgaa tggggacatc caccttcatg acctgggttt catcgacaga
    14341 ccatattgta gtggccagtc cttagaatac ataaaaaaat tcggtcttga tctaccgcat
    14401 tctctttcca tggcaaaacc ggcaaaacat cctgaagttc ttcttgccca tcttgtaaaa
    14461 tttgctgcag cacttcaaag caactttgca ggagcaattg gatgggatgc tgtcaatatc
    14521 ttttttgccc cgtacttgga aggcctatcc gacgatgaga tgaaacagtt tgcccaaatg
    14581 cttatttttg agttttccca gcaggcagtt gcccggggag ggcaggcaat tttttctgac
    14641 atcaaccttt actgggaagt accaaaacac tttgagaatg tttcggccat tgggccaggc
    14701 ggtcagttta caggtaaaaa atacggagac tacgaaaaag aatcccagag gttcgcctgg
    14761 aaactctttg aggtttataa agaaggcgat ggtgctggaa gacctttttt cttcccaaaa
    14821 cccctcgtac atattacaga aaagtttttt agaactgaag gccatatgga tttcctgaac
    14881 catattgcag acgtatcagc agacaaggga aatacttatt atgtctttga cagaggagaa
    14941 acggctaaga tatcagaatg ttgtcgtctt agcttcaaac ttgagaaatc tgatctcaga
    15001 gatgcaatgg agccgtggcg catgagatac tgtgcccttc agaatgtttc actcaacctt
    15061 cctcgcatag cctatactgc tcaacatgat gacacaaaac tcatggatct cattacccag
    15121 aggtttcttc ttgccactaa agcccacaaa gaaaaaaggg ctttccttga aaaactactt
    15181 gcacacgggg aagatggacc tctcgctctt ctcaccatga atagagacgg tatgccttac
    15241 ctgagatttg acagggcagc ccatttgatt ggcatggtgg gtctcaatga gatggttcag
    15301 atacatacgg gtgaagagat gcacgaatcc aaacaggccc ttaagtttgg cctcaagata
    15361 atggcccata tgaaacttct tacggataaa atacagcgtc aggaagggtt taaaatagtt
    15421 cttgagcaga ccccggctga aagcacaagt taccggtttg ctcgtttaga tctccattat
    15481 ttttctccct cctccgggag agttgttaaa ggtgacattg catctggcga agtctactat
    15541 actaactcga cgcaccttaa tgttggctac cccatgaatc ccattgaacg ggttacaaac
    15601 gaaggtatct ttcatcctct tattgaagct ggatccatct cccatgtatg gctgggtgaa
    15661 tcgcatccat acaaacaggc ccttgctgat tttgttatca aaacatttcg ccatacaacg
    15721 aacgatcaga tcgccttctc gccagaattc acaacatgta ataaatgtca ccgcacatca
    15781 agagggcttg atgatatctg ttcatactgt ggttcatcag atgtggacgg tatcacaaga
    15841 attacaggct attttaccaa ggtttcaagt tggaataaag gcaagctcgg agaattaaaa
    15901 gacagacata gggtaggtga atattttaac gaacagcaga aagtggcaaa tggataaaac
    15961 aatgttggat tttgaatcat taattttgag ttctaggctc atagcttata atccaacatt
    16021 aaaaattaaa attgttttta tgggaagggg taagcgaaat gggtattgta aaaatctttt
    16081 ataaggatga ttgcccaatg tgtccagtgg caaaaaaact tggggagcac cttgctgcgg
    16141 aaaatattgg tgttcaacat tataatgtgg aaacagccga tggccttgcg gaggctacgt
    16201 tctatcacgt tatggctctc cctaccattt tggtggaaga tgaagatgag aatggaatcg
    16261 gtgaatggag aggcagtgta ccacgaattg atgaggtttt gaatgccttc aggaatgcat
    16321 aagttcccca tcaagggttt cattgagaca agctttcttg actggaaggg agaactttca
    16381 tcagtcattt tcttcgcagg ctgtaacttc aggtgtcctt tctgtcataa tagcgacctt
    16441 gttcttcgct gggataccct aagtgacgtt catctagaat acatcatcct cacattaagg
    16501 aaatataaac agtgggtgaa caaagtggtt gtcactggcg gagaaccgac cattcacatg
    16561 ggtctctaca acgtactcgg ctatcttaag cgagaagaca tacaggtgaa actcgacaca
    16621 aatggctctt ctccctcagt tctgagaggg cttgtagctg aagggctcgt tgattatatt
    16681 gctatggatg ttaaagggcc tcttgaacgc tacgatacgt ggtgtggaac aaaggttgac
    16741 attaaaaaga ttgaggaaag tatccgcttt atcatggaag gaaatgtgga ttatgagttc
    16801 aggatgaccg ttgtcccatc cctgcatcgc gaggaagatg tataccaggt tgccgagtac
    16861 ctgagggcat ctgataagtt ttttctccag gagtttaaac cgagacaatg tattgatagg
    16921 tccttcctga acgtacagcc tttcccgaaa gatgtttttg agcgtatgga caagaatgtc
    16981 cagcaaataa gagtcaccgg tataaaacca gaagagcgga ttattaagtt tcgcgaaaaa
    17041 atattaaaac cagatcgtgc ggacagtaaa taaattatct atgtcgaccc tatttattca
    17101 agaaagctct cgagcctctt tctacgagat ggatgtttca gcttacggag tgctttcgcc
    17161 tcaatctgac gaatcctctc tcttgtgagg ccaaaaacct cgcccacttc ctccagggtg
    17221 taatcagtct tttctccaat accaagcctc atcttgatga ccttctcctc ccttggggtg
    17281 agcgtggaaa ggactttgtc aatctcctcc tttagggaaa taccaacaag ctccatgaag
    17341 ggtgagggtg attttgggtc agcgataaaa tctccaagtt tggactcatc atcgccaata
    17401 ggcgtctcaa ttgaaattgg ttcattggaa accttcataa ttttacgaac tttttccagg
    17461 gggagccctg ctttattgga tatctcctca aggttgggtt ctcttcccaa ttcctgatat
    17521 agggatatag ttactttcgt gatcttgttc attgtttcaa ggacgtggac aggcacccta
    17581 atcgtccttg cataatctgc tattgcacga gttatggcct gacggatcca ccatgtggag
    17641 tatgttgaaa atttatagcc tttctggtaa tcatacttct cggcagcttt cataagcccc
    17701 atgttgcctt cctgtattaa atcaagaaag gatagccctc tgttaaggta cttcttggcg
    17761 atattgataa caaggcgaag attcgcctga ataagcctgt tcttgactat cttaaggcta
    17821 ttttcaattt cactgatttc attaagtctt ttccttatcg tctttgcctc tttatcgtca
    17881 aggtagcgaa cctgctttgt cattctcctg atcatctctt caagaacctt cttgttcagg
    17941 ttaaggtttg ataaggattc ctcgatcttt tgatctgtac ctttaatctt tctttcaatt
    18001 tgttttttgg cagcttcatc acccttggga actgatttgc ggaattcttg ttttttttcg
    18061 aattgagact tgatagtatt aattaaagaa agggtttttt tcttgtattt ctcctcatct
    18121 tttttcgtgt agttcatctc atcgatattc ttgaccacat cgatgatatt tatagactct
    18181 ttcttaatga gcgacccaag ctcctggagt tccctgatcg cataaggcaa gtcaaagaga
    18241 agatttcttg ttttcctttc tccttcttca attctttttg ctatcatgaa ctcctcatca
    18301 gaggtgagaa gagacaccct accaatatcc tttaaataag cccagataat gttgtcagct
    18361 ctctcggaag ggaatcgctc gctttctccc cattcttggc tttcctctgc aggggcttca
    18421 gtcttttcct tcattgtgtc gacaatatcg atgtttgact ctgtgagaaa atcgaagatg
    18481 tcttctatgt cctcaggaga aaaaagattt tgaggaagaa catcatttat ttcgtctggg
    18541 gtaaggtaac ccttttccat ccccatgtcc acaaggtgtt ttatttctgt gaaatttttt
    18601 acgctcattt atgaccccga aatgtaaaaa atgccctttt cctaagggca ttttttacat
    18661 ttattgatat gctttatcca actgctatta agtatagcaa tggcaagggg attttgtcaa
    18721 gcaacacgaa tgaataaaat atttatggat tgacttgaac tcggagaagg catataagaa
    18781 atataagatg ttacaaggag gtgcttatgt ttaagaaaat actctgccct gttgattttt
    18841 cagagtttac agacgaaata ctttcttatg ctgaaattat tgccaaacgt tttgattcag
    18901 agctccacct gattcatgtc ataccaaacc ttagctattt caccccttac gaatccttcc
    18961 tcacccctga aaacctagtg gcaattgaaa gaaatattga aggagaggta ggaaaggatt
    19021 ttgacaagat tactaaaaaa ctcgattttc cttttaaaag aataattaaa accggggtaa
    19081 cctctgttga aatcattgac tacattaaac aacagggcat tgatcttgtt gtgatgggta
    19141 ctcatggccg gagcggtata gaacacatcc tcattggaag tgttgcggaa aaggtggtca
    19201 ggaagtcacc ctgccccgtg ctaacagttc gccccaagag taaaaccttt tagaccaagg
    19261 tataaggtcc aagagaaaag ggaaatcata ggtcaagcct cgagcctatt tagcatgttc
    19321 cttttgcttt gtttttgatc ctattctggt ttcttgcaga gatcatttat gctgatggag
    19381 tcaaagtatg ccatgagttt gcgtgaaccc tcctcccaag actcctgcac tatgcagtaa
    19441 ttgagccgct cacaagtttt tgcagcattc ttcttcagtc catcgcaaaa tacaagctta
    19501 aaatcacccc cttcagttgc ccgtatcaca tcgcctattc tgatttcact tggatctttg
    19561 gctaggtagt aacccccaca aggccccctg atgcttttaa caatgcctgc tttcttgaat
    19621 ttttgaaaga tttgctctat gtaacgtgga gaaatccctt catgttctga gatgtgttta
    19681 acctgaatag gattaccatt tgaattgaat gagatataac aaagagcccg gacaccatac
    19741 ctgatttgcg tattgatctt cattttattc cttcattttt tatagaatct tttttgtaaa
    19801 caggttacgc atcgtttttt tcaacgagtt gcttgtgaag atcccgaata gtttttaaag
    19861 gaattgtttc aactccaggg attctttctg ctccccgggg acataagacc cttttgatac
    19921 caagcctttc acactcttta agtcgtgcct ccatattaat aacttttctg atttcacctg
    19981 ttagtcctat ctcaccaaac agtgcggtat cttttccaat attcatatca ttatagcttg
    20041 acaaaattga tcctgccact gcaaggtcaa tggctgggtc agcaactttc atccctcctg
    20101 ttacattgat atatatatct ctgtcaaaga agggtttttc gagtactttc tcaagcactg
    20161 ctatgaggat aaacaatctg ttaatgtcat aacctaagga aagccgtttc ggcatcgaaa
    20221 atgttgtctt tggcgtaatc gcctgaacct cgagaacaat gggtcgagaa ccagtaacgt
    20281 atgggaaaag agtgcttcct gaactgatat cgcctcgatc tgccacaaaa aattcagagg
    20341 gattttcaac gctcacgagc ccttcggtgc gcatctggaa tattcccact tcatctatag
    20401 gtccatagcg gttcttaatg gctctcagca ttctataggg gagcgtctta tcgccttcaa
    20461 aatacaggac agtgtcgacc atgtgctcaa ggattttagg ccctgcgata gctccttctt
    20521 tcgtcacatg gccaataaat acgtgggcag tctcagacct cttcatctcc attatgagcc
    20581 gcgtggagac atccttgatc tgactgacgc ttcctggaag cattggaagc ctcgaattgt
    20641 atgttgactg gatggaatct atcactgcaa atttgtaatg tttatcgctg actgccctca
    20701 ggatatcttc aacatgattc gttgcaagta ttggaaagga acccttaagg tgcaaccgtt
    20761 tttttcgtga agacaattgt cggagagatt cttcgccaga tacatacagc gcatcaaatc
    20821 caagctccac cattttcgaa gctatctcga aacaaagggt tgtcttgcca attccaggat
    20881 ctcccccaag aagaatggaa gaaccacacg taatccctcc tcccaatacc ctatccatct
    20941 cgtttattcc aagaattatt ctttcctcag gaagatcttc ctctgaaaca aaaatcggct
    21001 tttcctgctg tgatagttcc tccttctcaa tacgaaattc tttaattgtg tcccaaccac
    21061 cgcattttgg acacttaccc atccacttaa aggtttcatg tccgcaggcc tcacaagaaa
    21121 aaactgtctt tttagccatt gaagtccttc ctcagatgcc ttctcataaa tgccacctgg
    21181 tacttaagca tcctaactaa ccactctctt gtacgaggga gttcatgcgg tgaaatctcg
    21241 agggtaacca tcccttcgta acctaatctt cctatagtat tgagaagttt gaccatgggc
    21301 aactctccgc aaccgagaaa aaggtggctc tcgtaatcac cgaagtcact gatatgaatg
    21361 tttttgagcc ttttcgtctt aaaaaatttg agaaaagcat caataacatc attaccaaag
    21421 gtggccaaat gagtagtatc aaaggtgaag tagaggttct tgcttacacc gaactcaatg
    21481 agatctttga agctattcaa aatgtaaggt gcaagtaaaa ccctttttcc tatgcgaggc
    21541 atattctcaa ttgcaaggat tactctatca gcgccgagtt ttgactgaaa atcctgaacg
    21601 cttctaaacc aacggaagaa tgcaacttcc atggaaaacc aattgggggg gtgaaaattg
    21661 acgatttcag catcaagagt cttggcaacc tcaacggatc tttgcaaaga gcaaacttgg
    21721 ttaccccaag tggccatctt catgaaaggg gcatgtattg aaaggatagg aaggatttcg
    21781 cgacattgca acgctgtatc gatggagcga ctatcgttga atctcctgtc tatgaccagt
    21841 tcacaaccat cgaaacctgc ttctttggcg aggaggaaca catctttggt ggggaggtag
    21901 taaaggcagc ccgttgaaaa aagaatcttc atacaataat gatatgctca tcagtgtttg
    21961 taaggaccct ataacttcct ttttcaataa gcacagtatt ttctatacca caagaacctt
    22021 tttttggaaa gaccatcttt ggctcaatcg ccatgaccgt tccctcattg atggaataat
    22081 tatgattggc agcaatataa ggatactcag cgagttcaat gcctatgcca tgagcaagaa
    22141 acctaacttt gtgatgtcca tagccaaggt aatactcggc atagcccatt ttttccgcga
    22201 gttgcttgga aaactcaaag atatctttgc tgacagcacc ttcaataatt ttgtcgagga
    22261 cccgataatg gatctctttg catgcctcat aggctttaat gaaaaggtca ggcatagagc
    22321 caatggcata catccttgtc tggtccatgt gatacccgtg gtaacttatg ccaaaatcca
    22381 caaggatcgg ttcatttttc ttgatctttt tgatgcttgc tccaactgga aaggccgggg
    22441 aaagccccaa gccacccatg ggagagtctg actggcttac aatgcttccg gttctgccgc
    22501 ttaaaatatg ccatgtatag gcttcatagt taagagaacg aacacggaga agcccttcgt
    22561 gcccatgtgt tttagcgtag gcttcaagaa tcccgccgag ctcgatctct gtcatacctt
    22621 ctcttagaag ctcaggtatt tttttatata ctctctttga aatctctgca gccctctcca
    22681 tgagcccaat ctcaaaagga gattttactt tccttatttc ctggatcatt ggtgaaacat
    22741 tcaccatttc cgcgtcaccc agatactcct ttactctgat aacatcattg taaggtataa
    22801 cgtcgagttg gagtccgacg cgtttcatgg gcttgatgtg ggtaaaaatt tcttttatgg
    22861 atcgaaaagg agttatctgt ttgatgggcg attcccttcg cgcccgtgat atctctcttt
    22921 ttacaaaaag gattggctca tcatcagatg gtatgaaaag tagcgagtct tgcatggtac
    22981 ccgccaaata ataatagtca atcttataat ggaaaaaggc cccgtcaaga gaggcctcct
    23041 ccatcaattt tttcaccttg cttatccgtg actctatttc ctcccggggg acatacttca
    23101 tggtgtgaaa cctacttgtc tttccagact gccttgcgtt tctccataaa tgcattgatg
    23161 ccttccttgg catcttctgt ggccatgcag cccttgttgt aaattatctc ggatgccttc
    23221 agcgcttgga gaaaatccag actcaagcct gctttgatag ctcttttcgt ccacattgct
    23281 acgggtctgc ttttgttcag gaagtctgca agatattttt ctacttctgc cttgaagttt
    23341 tccacaggca gcacggctgt gaccagccct attgcctggg cctctttcgc actgattatc
    23401 ttgcctgtca aaagcagttc atacgtcttc tggagaccga tgatcttggg gaaccaggcg
    23461 gctgccacag gaggataaac acctacagcg atctctggct gacctattct tgccttttct
    23521 gaagcaacta cgatatcaca gaaggccata agttcacatc cccctccgag cgatcgtcca
    23581 ttgacaagcg ccacggtggt caggtccatt tcagcaagat gcctgaacat tctgtggaaa
    23641 acatcgatca tttcgtccac tttgtcttcg gtgtggtctg ccacgtctac tccatcacag
    23701 aaggcttttt cgcctgcgtg atcaaaaatc agcatctgaa tggaagcatc cttttttacg
    23761 tcgtcaagca cttcgttaat ctccttcatc atggagattg taagccaatt tgatggtggt
    23821 gtatttagac tgattgttgc aactttaccc tttttttcaa aagtgatgtg attgaaagcc
    23881 attgttttct ccttgtttaa agcccataac gcgtgacacg tgatgggtgt ttgaaataaa
    23941 aaaagggggg atttctcccc ccgtgaaagt tacctttctt acttgccctg cggtttcccg
    24001 agaacctgtt ccataaaagc tgtatcgacc agtttgcctt ctgcaatgag ctggcggttc
    24061 ttgatgaagt cgatggtgtc cacaccggtg atcttcttgg tgttgaaggc gccgaagcca
    24121 aggaaggcct cgcccatcat gttggttgcc agccagtatc tgtggtcgtt cttgatctga
    24181 tcccagaaga atttcttctt ctgccgtacg ccgtcgatgg atttcatcag gcagccgggg
    24241 aacagattcg cgaagatcca gacgattcta tcaacttctg catcgaggag ggagaagtcg
    24301 acttcgcctt ttttctgttt atcctgaacc catgcccgtg catccttgaa ttcctggcct
    24361 gttttcatct caccgtacac gatttctcca tccttgacga acgcgtcggt tatgacctgg
    24421 ggattccgca cccagtttcc cttgtcatct ttcaggaccg ggaaggcctt gctgatgagg
    24481 cccttccacc acatcttgta ggcgctccac atctcgcaac tcacacagct ccacatggcg
    24541 tcttctacac ccagatacca cgggaggaag tcagaagcgc cacccacagg ggctgaaccg
    24601 tgacggggac ctgcctgacc taaaattgcc aggtctgaag agatactgat gtcgcatgct
    24661 gttccaattt cctggccgcc cgcaaccctc atgccgttaa tcctgcagat gaccggtttt
    24721 ttgcacataa ggatactgtc aaccatgttg ttgaaaagtt ccatatatgc accgtattct
    24781 tccggtctca tactgtaata ttctgaatat tccttagtgt tgccaccggt acagaaggcg
    24841 tacggtccta ctccagtaaa aactgttgcc acaaccgagc gatctgttga ggagttctca
    24901 aaacccgcaa tcacaccctt caccatctct gtggtgtaag agttaaactg cgcaggattg
    24961 ttgagtgtga cccaagctac gtaaaggcct ggtattacat tgcccttggg gtccttcaaa
    25021 ggtctcttct cataaacggt acatggagcc tctgtgcccc accactgtgt accatgtctg
    25081 ctgtggtctt tcatctcatg ttctcttggc atccattcta aacccatact ttcctcctaa
    25141 atagaatttt attgttgctc caaactactg cttaaaagtc tggctttaag gaaaccctct
    25201 ttgcagggga tccagccttg tggatctcat caaatgttgc tgcgatcgtg ctcatgggcc
    25261 taacctcaac aaaaggatcg atgacgatct tcttgttcag gaccatatca agaacgatcg
    25321 ggtagtattc tggcaaacaa ccccatgtcc cgataatctc tgcatcaaag gccattagcc
    25381 tcgaaaacat atattcgctc cttgacagac caaagcctac tacgagaagc ttgccaacaa
    25441 atgataggag atcaagcgca aggtcctgac ctgccttcgt gccggtaacc tcaaagattt
    25501 tccagcctgt gttgtcaaga cctctggact tgcaatagtc cctccactct tttacaagag
    25561 ccttgttgtc tttttcaagg gtgctgattg tgtaatcaca gccatagtcg agtgctcttt
    25621 tcagtttgtc aggattccgg gcaataccga taacggtctt tgcaccgagt gctttaactg
    25681 tctgagccat ataaacacca acaccacctg tagcgccgat aacgattacg ttatcaccag
    25741 gctgcagatc tgccctcttg catgcctgat aaggggttgt aaccgcatca gccacaactg
    25801 caagctgttc aagagcataa ccctttttat ccgcaacgag gcaaaggtca atagaaggga
    25861 ccacaatatg gcttgcaaaa ccaccaaaga cacccatgct gttaccaggc atcttctggg
    25921 ccaaacacct gtttccacgg cctgttttac agaggataca tttcctgcat ggcaaaactg
    25981 caggaatgat aacctctttt ccaacccatg cagggtctcc agctatcaca gtacctgaaa
    26041 tctcatggcc aagggccaac gggggtttgc ttacggtggg cacgccatca taaaaatagc
    26101 caaggtctgt gtggcataca ccgcagccgg ctatctcaat cagcacttcc ccttccttca
    26161 gttcaggtac cggtatttca accatttcca gctttccagg ggttgtctct ttcgtttccc
    26221 tgttgtatac cgtaggttgg gtcatctgcc actgttttat tgtcttaggt aaatctgcca
    26281 ttcaacacct ccttatatat ttgaaatact ttcaatacca cctccttacc atattacgtc
    26341 attccataac caccgtctac aggaagaacc tgccctgtaa tatatcgagc gtcatcagaa
    26401 gcaaaaaaca caaaggccgg ggcaacatct tctggttctg catacctgtt gagcagaatc
    26461 cttctaacat atatctcttt cagcttgtca tcggtttgga gtgtcattgt catttcggtt
    26521 gttactatac caagagatat tacatttgcc gtaacgttga attttgcaag ttcccgcgca
    26581 gctgacttgg tcagagtcac aaccccgcct tttgccgctg catagtttat ctggcctatt
    26641 gttcctacca ttccagctac tgaggtgacg ttcacaattc ttccatattt ctgctccatg
    26701 aaatatttgg atgctgcttt aatcatgagc caggggcctc tcatttgaac attcacaacc
    26761 tcatcccaca catcaaccgt caatttgtgc agcatggcag gcttcgaaat tccagcatta
    26821 ttcaccaaga tatgaatgga tccaaaattt tctacacatg catcaacaat cctctttgca
    26881 tcctcttcct ggcccacatc gccctttaca gcaactgcct tccgtcccat ggcctggatc
    26941 atgcccacca cttcgtctgc cgccttctga ttaccggcat agttcaccac aacatttgca
    27001 ccttcctggg caaaggctaa actgatggcc cttccaaccc ctcttccacc accggtaact
    27061 atagcgttct ttccttctaa tttgcccata acgcccctct ccttttcatt ttgtgaaatt
    27121 tttcgtaaga ctaatattat agtttaatag gaatatatgt caagacaaaa agatatattt
    27181 acagtacttt atgaggggtg tgcatggccg gatcgtgcat ttttgcactt aaccaggttg
    27241 tcttcatgca tccagcatac taagtacagg taggacagta gattaagaat cccacgacac
    27301 aataatcccg atgatctttt ggtgctcaat ccagtcagtt atttttaaat atctcttcat
    27361 agagattcct gagcaacaca ttggcaatat atcttggaaa taacattgtc tttttgccag
    27421 acctatggta cagaatatga gcaacttttt ctaatacctt accggggcgg atggtgttat
    27481 gaatcaaaaa cttgcccggc ttttttctcc tcattccgta gcagtggttg gtgcatctaa
    27541 caactttgat aaactcggct atcatgttat gaaaagcctt gtcggtaact accgaggaaa
    27601 gatctaccca ataaatccga aagattacaa aatctggggc ctagattcat atgcttcgtt
    27661 aggtgcaatc tctgacaata ttgacttagc ggtaattgtg gtaccagccc acatggtacc
    27721 agacgcactt cacttatgcg gggggaaggg ggtgcaaggg gtagtgctca ttacggcagg
    27781 atttcgagaa atcgaagatc ctgggggcag tgtccgcgag gaagagattc gggaaattgc
    27841 ggcacattac aatcttccta tcatcggacc aaataccttc ggctttgtca acctgagttc
    27901 tggggtaaat gcctctttca cctctgagtt ctcgagaatc gagaaagggg gtgttgccct
    27961 cataagtcag agcggtggca tttgtcatct ctgcggcttc cttgccattg agcagggggt
    28021 cgccatgtca aaactgatga gtcttggcaa ccgttgtaat gtagatttcc ctgagttact
    28081 atcctacctt gttgaagaag acagcacaac aagagtaatc gccctttaca tagagggact
    28141 tgataaacca cgaaggctgc ttgaggttgc tgagtcgctg cgtggaaaaa agcctgtaat
    28201 cgcatacaaa gcagggaaaa gcgaaaaagg agacagcgcg agccgatttc acaccggtac
    28261 ccttgccgga aaccatgcga tatggaaggg tgcccttcgt caagcaggaa ttttggaagt
    28321 tgatacagca gaggaattac tcgatacagc aaaagctatc gatgcatgcc ctttaccacg
    28381 aggtactcac atagctatcc tttccagtca agccggtcct ggtatgattg cttccgatgc
    28441 cctcgagcct gcggggttga aactctgccg tttttccgaa gttacacaag agagaatcga
    28501 tcagctttta cctcctgttg ccattcgtac aaatcctgtg gatatgggac ctgcgtggta
    28561 caatccaaag aatatcattg aaatcttgaa gacagtgctt ggggacagag atacagacgg
    28621 agtgatcttc ctggccatgt atgcctctgc taatctaaaa cttgtagagg gtatggtaga
    28681 atatatgaaa tccatagaat cttttcaaaa acctgttatt gcttgttttt cggccccccc
    28741 gggcgtatgg gatgaaccag ttaaagcact tgaccggaaa aaaggggttg taacccttcc
    28801 tacccctgaa agggcagtgc tggcaatggc gaatatgtgg aaagtcggct ttttaatgaa
    28861 tagaaaaagg taaagatttg gaatcattat tgcttgggat taacgccctg accttccttg
    28921 aaaaagaagg atttcctttg cttaaaagtt atcttgccca aaacgaaaag gaggctgcca
    28981 gccgctcatt agagatcggt tatcctgtga ccctcaaaat ctcttcacca gatatgattc
    29041 ataagaccga ggccggagga atcagggtat cacttaaaga tgaaatagaa gtgcgacacg
    29101 cgttcagaga gcttgttgat tcttttagtt cgtcttatcc ggaaaaatgg ctcgatggca
    29161 taatcgtgca gaaacagggt agtgggatcg aggtaatcgt tggaatgttg aaagatctac
    29221 agtttgggcc tgtcctcatg ttcggattgg ggggcgtatt cgtagaagcc atgaaagatg
    29281 tttcttttcg attgatccct attaacgctt ctgatgcaaa agatatgatg gaagaactga
    29341 agggttatga catacttagg aggtcccgaa gtggcccgat tgatctcccg gcaattgaag
    29401 attttctgct tcagttttca agatttattg ttcttcatcc tgaggttaaa gaaatagata
    29461 ttaatccatt gttcatatct tctctcggtt ctggcgtaca aatttgtgac gccagaatca
    29521 aggtggtcaa gtaatcttta tagggactaa caaaaaaagc atctctttca ccgaacgcac
    29581 tgtccagcgc ccataagaca agaccgctaa ttattgttgt tcagcatgaa tattcagaag
    29641 cctctttaac caccaaaatt ctctgctttg acaatctcac ctgaggcaac ggaaattatt
    29701 tccttaaaat tcggttgcct gcatctcgga ttctttcgct ttatcgaatc acaaagctca
    29761 tatttatatc catattatct aacatggtac ggtatctcta tttgcagtaa acttacgaac
    29821 aggcgattta gggttttcta tgggtggtgc ttcatcatgt ggtaaacacg acaatctgta
    29881 agtctcctgc tgccacactc gtgagttact caatgtcatg ggccgggata ttcatgttga
    29941 ccaaaccctc ttcgttgttc ttgcccattg acataactac ttctatgggc ttgttcctcc
    30001 tttgttgaca cagtctcgtg atttctctcc aagggttgtg ggccctacct gctatgctgt
    30061 taggagatgg gtttccttcc cgaatgtcct ggactgcaac aaccgactga cactcccacc
    30121 attttattgt actgaagatc cgcctagcta tctcaggact ctgcgccctt ttacaacaag
    30181 atgttctgcc ccgattaact ctgatatctc ggaaaggatc actgttcagc cctcagcaac
    30241 caacatcaag tcacatgacc ccgactgccg gatttgctac aatgcctgaa gcatagtccg
    30301 ttccacccaa aatattgggc agaaaaaccc ccttcgatac cgtgacaata acccatgcag
    30361 caacacataa cattggggat gatggatacg tgattgcctg ttcctgcttt cctctaagct
    30421 attaaatagc cctggaaact attcattatc ctgagctttt aactgactga ccacaatgcg
    30481 gatattctga atctcttgtt tccctgttct gaaggataaa tttgtaatcc ctgggacacc
    30541 tgaaagcggc gttatggagt actaccgctt ctgtatctct tttaaaattt gtattcccct
    30601 ggcacgcgaa gtcagggctg ttgtgacatt nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    30661 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    30721 nnnnnnnnnn gatgatcatg taattgttaa tccgtaactg gcacaacaag tccgccagac
    30781 ggaaatattg caactgttgc ctctggatat gccttctcta gaactcgcaa tccttgtttc
    30841 agggagctgg catattccat acccattgta ctggcatctt ccctcgagat cattggtgat
    30901 acagagatgg tccgtatggg gcggcgtaga atcatacaag acattgccat attgaactcc
    30961 aaaggctttt ccggcagaaa aggcaaaccc tgtgccatag cattccttac aaatcgttct
    31021 ggttgatttt ctgatttttc ccgaaccaca ttgaaggagt cccgaaactc atcgggtacc
    31081 ggtgttacaa ggggtgccat tagaagtata gcaccagtgt ctttggtgac agcatcaggg
    31141 gcactaaaac ccttggcaaa ctggtggcca tgagtgtggg gataagtgga actaattgtt
    31201 atatcaactt tttcctgaaa tctgtggcca agctcctcgt agcatagctc aacggcttta
    31261 aaaaatgcgg cctctgggct tcctgcgata atctgaactg gttccccctg ttgattgtac
    31321 acgcagttaa tggtaaggga gagccctaca gttcgtgcca cttccaaaac tttctcatga
    31381 aaagggtttc cttttatcaa tcctgcacgc gcctgtggat ggatagtgta tctcatgtgg
    31441 tgatcccgtg tatagtcaaa gtcagctatg ccaggcatga caatctttgg tcctccacca
    31501 taccctgcca tagggtgggg caagatagaa ctgataccta ttttcaggtc tgcctggaca
    31561 acgtggggat tgatcttcac aatttggttg ccatcgagaa caaaaggtat gagattatcc
    31621 tgccaggcat tatgttgaac aatcctgtac cctgacatca aatcactgcc aattcgtaat
    31681 tccaggtctt catgctttac gggcatgtgg gttccaaggg caataacgag ggtaatcttt
    31741 tcttttgtga ctcctttctt ggcaagaaga gggagcaaca cattgagaat ccttttcaca
    31801 ggcgttggcc ttgttgcatc atcaatgata attgcgacac gacaaactct tccggtaagt
    31861 tcgtccagcg acggactgcc aatgggctgt agaagtgctc tttctgtcat ctcctcaatg
    31921 gtgggcataa agactttctt ttctttttca acaaaatgca cgacgttcca cctgttcgaa
    31981 agggagaaaa actcttttct gttagggcca agacagagat agtatcgagg tttcatgatc
    32041 agctcctgta aaagactgtg tggttttgcc gtaacataat agcataaaaa taccctcaac
    32101 tttttcacag attgttacaa cgtttcattt ttcatcaaag ttcttatgga tagactcaca
    32161 agcatgagtc ctctgcaagc acgactggct tcaagtcagg tatctttata accgtcaggt
    32221 ggtcctgaaa ataagaagta ttaaggaagc attgaaaata tatcaggctc tgtagccata
    32281 ctgtttcttc ctccattctt cacaaaatag agggccgggt cagaccacaa cgagaatgcc
    32341 tttggagatt cgttcaagca atagtacgct actcctgttg aggcggttat tttcccaata
    32401 tttcgtaagg ttgctatctc tttcagagtt gcttgactaa aatactttct catatttttc
    32461 cttcaacctt agaggcccac aaagaggtta cagggaaaat caccgcaaat ttttcacctt
    32521 caaagcgagt caaaannnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    32581 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnntttcc
    32641 ataattttcc aatatcttca tctcatcaat gattgccctg acaatctatt taacctcttt
    32701 ttgagatgaa ttatcagaca atatcctggt tgagtctgag ataaacgaca catatttttt
    32761 tgtttgccca tcaatttagt aacttcctgt aatataacgg ttaaaacctg ttggagatta
    32821 tctcgtagat tcttcaattg attttcatgt ctcagtacaa tatcacctat aagggggatt
    32881 cattagtttc ttctgaaaaa ggcttcttct attcaagtat ggagtctatg ctgtttctca
    32941 atttactatc tgcccctgaa actatacgta taccaaactg caaagttctt tggcgttaca
    33001 ggcacttcat gctcaaatac tcgtatgctt tcttaatcag gctgttttca cacattgtgt
    33061 gtatattctt tttcagtcat cgttttctca atatttctca tgctacagta gaaaaatatt
    33121 cactgaacaa acacaacaat gcaaatcttt ccgtcgcttc atgatacttg atggatgcgg
    33181 ttatgtattc aaacaaccaa gtaactggtt ttctatgggg tttattattc tatgctggtc
    33241 tcctgttaag ccattttttc tcggttcaag attccatcca ccactttcca agctatttct
    33301 tcaagggtcc ctggtgtgat gggcttccaa accttgcgaa tatctgctgt tgcctcaaca
    33361 aaccgaccac atgtgctttt ataaaattgc ccgtatcgga taaacacctt atttgaaagg
    33421 ccaatacccc acttttcggc aatacattca agacttccat atcctgctct tcttatatac
    33481 ccatgtgcag tgttgaatcg tgtacttccc tcaggagggt agatgtttac actgatttct
    33541 attttatcct tattaaaagc aattgatttc ttgtctaaat aaactaatgt tccatccttg
    33601 tctttcttga ggagtattcc ttctttttca gttatagatt ggtctgcttt tttttgttca
    33661 ggcaattttt gagttaatgg aggggctcct ttctgatctt tttttcggag gcccatcaat
    33721 tcctggtaag ccacctctac tttatcccgc ccttccattg ctttctgttt atagaaatcc
    33781 gggactcgat caggattcca gtattccatc aataccctgt atgcattcac aatttcttca
    33841 tgagacgcgc ttggttttaa tccaaggaca tccagaaagt gttcccgatc acacatagct
    33901 ttgagacctc accaaatgtg ttatcacact gattattgga tttcagcgag aaatatcacg
    33961 ttattcctgg caaaccaaaa gagataaaga gagatgcgtt tctgcgggga tagccggaaa
    34021 ccaagcctcc attgcttgct tatatttgat gaactgaaac tagcaatgga ttgcaacagg
    34081 ttatggaatc gtcacgatac gggaggcctt attgttttct tcatttgtct ctgccactct
    34141 gttctccagg tcaacagata ctgtaaaaag gttatcacca gcattcagcg cgataccccg
    34201 tgtaacaaac ttttttatta tgttaaaacc tggttttagc tttttaacct tgagtggctt
    34261 cttcaacatc atgtggagag catcggtgtg ataaacaccc ataaggcatg cgttggaagg
    34321 catattcccc cggttgctca cagtaaccga gccctttact ttatataggc cttttttatt
    34381 tgggccagta agggtgatct gattccattc accaacgagg tcaggtccca gcaacacagg
    34441 gggcacctca ccgttgctcc cttcccttag atgtgatcct ctacctgtcc cccatagtac
    34501 acgggcgctg gctgttcctg gaagatcata ggctacaaaa cccgaatgga cggtgttcaa
    34561 aaccacctcc aactcgccat ctccatcaat atctgcaaga gttggagcag ccagggcccc
    34621 attccagtct acccctccga aagcatacgg cagatcaacc tcgtggagaa cataacccat
    34681 ataatcaaga atatgcaatt taccagtggc attgctgcct ttctgggccc atgatgcgaa
    34741 tattacctct gcatgtccat cgttatcgag gtcagccaca accggctcag atgcaaaacg
    34801 gtagaatccc tccaccagat tgtaaactga atacggccag ttacctcgct ctgtcttatc
    34861 aagccaaaag cagtgcattc ttccatctga tgatggatag agaatctctg cccggccatc
    34921 cccatccaga tcgactgtca ccgggtttgg ttgcacgctc tcgatcacat tgtaatcatc
    34981 gctcaggggc gcccctgtat tgataggtat agtggtccag ttgaacccgt ctttgttgaa
    35041 ccggcttcgg tcagcgttga agatgaaagg ggtgttgtac aggtttgtat agggatcggt
    35101 attgcagttg tgaacgtaac ccacaacaac gacctctttc acgccatcac cattcatgtc
    35161 tacaacattt gccggcccct gggcaaagtt ggcacgtgct gtaaacccat cgtaacatgg
    35221 cccccagcca cgagcttcat actcgaggtc aacataggct ggaacttctc cccaggcatc
    35281 catatcgtga ccaggattgc catgatacat ggggttggtg gaaagctgcg tgccgtcgga
    35341 cttgtatgcg ctcaccgtaa ttgtgtcaga gggtataaca atttccggca gcccatcccc
    35401 gtcaagatca ccgaggccga tgtttgcgtt gtatataccc caggcagagc tttcgtcacc
    35461 agccttcagt ctcggccaac cctggcgtat ttcccccgtg tgctcaagta cccatgtgtt
    35521 tgttttgctt cccgcagcac gacccacgat tatctccaaa aaaccattgc catcgaggtc
    35581 tcccactgac agactccgaa actcctgccc tcctgggttt ttccacggcc atccgcgaga
    35641 cttgaaatat ccatccctgc tcaggaccga tacccacccg cctccgtggg cagttacaat
    35701 ttccagttca ccatcatgat caatatctgc gacaataatt ccgggccatg tacgccccac
    35761 gttttctgcg tttggttcat ttctatcgtg accggacttg acgcgccact ttagggctcc
    35821 tgtttcacca ttaagaacaa caattgaata tgctgatgct atgacttcca tccttccatc
    35881 cccgtcaagg tcagcgacgg caggtgatga gtaccatccg gtttcgcacc aggaggaaaa
    35941 gcacccccca tgttgccact tgagaaccgg tgcgcttact gaagcatcaa ccattccaga
    36001 accccatact tgcatgatca ataaggttat aaaaaaaatt aaacccaaag cagcgcgtat
    36061 cattcctttg tctccttttt ataaaacaca attttcaaca aggattacct gtcataatct
    36121 tttgcgaacc agttctatac aaatattcaa acactctacc atctgcaccc ctttcaccgc
    36181 agtgaggcga atcaccctta ttgcccggaa ttacatggtc gggcaatata aaaccccttg
    36241 acaattttaa gtgatggcag gaaaattaag caataaaaag ttaaggaggt cgtaatgaga
    36301 ttttttaaag tgaagtattc catcattgtt tcttgtattg taggttttgt tttgagtggc
    36361 atcctggctt tcgccgcccc accagataat ttcactgcta caatagtttc tcagggaatg
    36421 gagatgccca tggcaaagat gggaaataaa agccgtgtgg agaaccctgc tataaaaggt
    36481 gttgtaacca tcgtcattgc tgatgcaaaa aaaacgatca tgatgaatac caatgcaaag
    36541 atatatagcg aacaagtcac tcaggagcag gatcagatga ctaacatgta cgatcctgat
    36601 gtggttttcg aaaaaaagaa agtggggaac gaaacaatcg atggtcatcc atgtgttaag
    36661 tatgacactg tttattatag aaagagtaag cctaatgaaa agcataaagc cataatctgg
    36721 gaagctcagg acctgaaaga attccccatc caaatggaag ttactgtacc agccaatccc
    36781 aaatatcccg gatcaggcgg aaagatgatc gtgaagtata aggatgttaa gttaggcgca
    36841 gccactgcat caatgtttga agtgccaccg ggatacaaga aggtcaacag cgtccaggag
    36901 gttatgggct ttggcggtat gggcaatatt gaagagatga tgaaaaaaat acctaaaggt
    36961 caacgcccgc ctaagcagtg agattataag aaagaacctg gaggaaaaac caaatactcc
    37021 aggttcttct tgctttatgc taggcaagac acagccggct tcactgctcc taggccatct
    37081 cgaattcgct tcaggaatgc aaggattgta tttatgtagt cctcggtacg ctgttcatac
    37141 gtgttgtgac cgcatcttgg gaaaacagca agttcgccct tagtcaagtg acggtagaat
    37201 gcaatggact gttcaacatc aaaaattgaa ctcctgtccg ggtatagtac aagtgtgggg
    37261 cacaaaacca tgtgtaggat gggcctcaga tcaaagtagt cttttccata agctcctccc
    37321 tgttttgcga actggttata cattttttca gctggctccc cgtgccattc gaccatcttg
    37381 gcctgtagtt cgggactgag ctcggcaaag gtattcacta gtgttttcac attaagctct
    37441 gtcataggta cctcactgta acactgagtg ctcgctatgg taagtgcctt gacttcctca
    37501 gggtacttga cggcataatc gaccccgatc acccctccct cacactgtcc aataagataa
    37561 caggagttta tccccagcca ttctttgata agccggagtt cctccacgct ttcaggtctg
    37621 tatcggtcgc tgtcgtaaaa cccgtaaaaa ccatcccctt tctctgattg accaaatcca
    37681 cgccgatcat acatgactat cctgaagcca tcggccacga atgaaggata aatctggttc
    37741 cagattttgc tgcagccaaa accatggtga agaaaaatga tagtatcccc ttctccatgt
    37801 acttcgaaat aaatattgaa accattaatt tttttgaaag gcatcaagaa ctccttcgat
    37861 ggcattttca attacagaat aagaagaccg acattatttt gaaacaatta actatatttt
    37921 ttgacattgt cccataatac gtgatctgtt tcaagtcaca atacgtgttt aattgtggca
    37981 ttgtacataa tccgctgagc cactcgggga agacgttcca ctccaattat ctgtgcagtg
    38041 agagtagggg ctcaatcagg tatacacaga ctgtcagttt gttagagctt ctttaacata
    38101 aaaccagata tatagttaga tcatagctct gttttaggaa ttttttttgt tataatcccc
    38161 ttttcttcaa gtaacttaaa agctcaacca tgctcttaag gacaatctcc ggtctggcct
    38221 ttaaaagcgt ttgctcatct tcatgcccgg ttgtgacagc tatggcgatg gctccagcac
    38281 tcttcgctgc aatgatatca gattctgtat ccccaacaac aatgcattta tctatcggta
    38341 tacccattct ttttgcacat tctataatcg catcaggtgc aggtttgcga tgttcaatct
    38401 cctgcgatgt cacaatcgcg ctgatgtatc tgtagaggcc aagctttttg aatcgtaacg
    38461 tttcatcttc agctgatgat gtcctgcctg tagcaatccc aatcttgatc cccgccgtca
    38521 taagatacct gaataattcc tctgtccccg gaaaaggttt tatcttttcc aactgagcca
    38581 ccctgaaaag agtgaggatc ttttccctgc acatgctgta tgtgacgtta tcgatgggtg
    38641 aggtaaatac attttccaga atttgacgaa gactcaaacc ctttgcaagg agatcgcgca
    38701 ggtcgcggcg ggatactttt tccacattgt aatcagcgat gccttcgtta aaaaccgtgt
    38761 agtacgcctc aaaactgtca atcaaggtac catcaatatc aaaaataagg gcctttatca
    38821 ttattttacc tcaaacctat ctacaactcc cacctaactc atgagaacaa gaatagccgg
    38881 gtgtttcata taaaaatttt gcagcacgct gaaagtttgg tcttttacat gaccaattct
    38941 actgaaatca gcccccttat cgggcgagag gggaggctct gtaaagcttt gtttgctgag
    39001 gagtgacgtg aaccccaagc attgcgctac tggaacgaat gagagtgttt gatgagaata
    39061 tccgacacag atgcgttctc ccatagaaaa caggattaat aatacggtag ttgctcatcg
    39121 aaacattttc ccttgttctt ataccacgca acaacatgtt ttatcgcttc aactgcctta
    39181 gccttcgcag caatatcaga attaaatccc tggtgtggtt gaacatatac attccctctt
    39241 ctttccaatg ccatgttgac aagggtttta gccgcaagaa attctttttc catcagtgaa
    39301 tcaccaagca agacttttgc caactcagcc tcgttggtga aaacatctaa gccaaggcct
    39361 gctatcttcc cggactggta taactccaat aaaaccgatt ctggtgcaat ctcccctcga
    39421 gttacattta tgaaaataat cccgtccttt gccatggaaa gatattcccg ggaaaaatag
    39481 cccacattga aaaacttgct ctcctttttt tttgtaaggt tcattgcgtt taccataata
    39541 tcactcgata tgacagcctc ttcttttgtg acaaacctga cagacctctc ataaatatca
    39601 ttcagttctt cctctcgtat atccaccccc tggacggtta aaccatttgc ttctgcaagc
    39661 tcataaattt ttctaccgat cttaccaacg ccataaacgg tgataattct gtgcttgtcg
    39721 agttccatga aggcgtttga gttctttctt tcgaaggtct cgatattttt tgtgtaatgg
    39781 ttcaattcac ctgctgtcgc atagagaaat ttcatcgcag tctgcgccac agcatttaca
    39841 cagtattccc tgagagaagc aatgtttgct gtttctgcaa ggtgctcaaa gtggtcgtaa
    39901 cctgcgcttc gggttataat actctttttt ggtccttcaa gataatcgtg tggaagaatt
    39961 gagtgggtct ttgttgttat tatgtctgga agaggatgac ctgggttttc cttgagaaac
    40021 tcctggagat tacttggtgt tataagatac gtgtggccta atggaagctg tctttgctct
    40081 ttcgcctttc ctatttcttc ttcaagatgt tgcgcctccg gacccaaagc ctcaaagtgc
    40141 ataatatcat aaactccttg ggctgtgatg gactcttttc tactattcat tgtagctcct
    40201 tcatagggtt gatgattcag gcaatgtcat attattgttg cttgcagttg tatttaatac
    40261 tgcctcaaaa acactgtttt ttcaacttca ttttcgattt cctaccagaa tataatgatc
    40321 ctgtaatgtt tgatattcat tttgtggagg tacggaatat atttctatag tataatgaac
    40381 aatctatcgc atataaaaag gacaggaagc atgaaagaac aaaaccttca cagcagcatc
    40441 tcgcacataa tactcggcac atgggctatg ggtggaaacc actggggcac atgtgacgat
    40501 gcagatgctc tccgggcaat cgaaacagcc attgataacg ggataaacac cattgacact
    40561 gccccggttt acggtgatgg tcacgccgag gagcttgtgg gtaaggctat ccggggtaag
    40621 cgggaaaaga tatttcttgc aactaaatgt ggactcgata tctataccaa caagtaccaa
    40681 cgaaacctct ctccatccta tatcgagcgt gatcttgccg aatcgctgaa acggctcgga
    40741 acagattata tcgatctgta ccagtgccat tggccagacc cacagacgcc cattgaagaa
    40801 accatgtcag cgcttatgca ttttcataag cagggaaaga tacggtacat cggtgtatca
    40861 aattacacat tggcagagct tgaggctgta atgcgctgca cgtccctttt ttcgctccag
    40921 tctcattact ccttacttga gagaaccgtt gagtctgatg tactaccatg gtgcagaaag
    40981 aacgatgtta ggtttctttc atacggttca ctcggcgccg ggatacttac cggtaagtat
    41041 gtggaatgta aacgcttccc ctcaggggat gcccgatctt ttttttatcg tttcttcaaa
    41101 ccacggtact ggccacgggt ctgtcagctc gttgatgcta tgaaagagat ggctctatcc
    41161 aggggagtca aaccaggggt gattgccctc gtctggttac tccagcagcc ggcagtaacc
    41221 tctgtaattg taggtgcccg ctcagcgcaa caagtacagg acaatattcc tctgggtggt
    41281 gtaaacctga cagattccga tatgcgcctt ctcgatcacc tctcgcaaaa agtctatgca
    41341 aaggagcaat gaaggggcta cccacctcgg gtcaaaataa tatgctttac tgcctatccc
    41401 aaatactccg tttaccctca ggaatcccct gacccccctt gcagtagaa
//
LOCUS       DAOOMC010000005        38502 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdus sp. isolate AMR_MDS_2685
            NODE_814_length_38502_cov_6.390720, whole genome shotgun sequence.
ACCESSION   DAOOMC010000005 DAOOMC010000000
VERSION     DAOOMC010000005.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864760
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdus sp. (wastewater metagenome)
  ORGANISM  Syntrophorhabdus sp.
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae; Syntrophorhabdus.
REFERENCE   1  (bases 1 to 38502)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 38502)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,729
            Genes (coding)                    :: 2,708
            CDSs (with protein)               :: 2,708
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 7 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38502
                     /organism="Syntrophorhabdus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_814_length_38502_cov_6.390720"
                     /isolate="AMR_MDS_2685"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2699756"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(421..639)
                     /locus_tag="PK125_00975"
     CDS             complement(421..639)
                     /locus_tag="PK125_00975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB36709.1"
                     /translation="MDCLCLYMGPDNVEHSQSPMGKEKATRSYSYPIIVGGKKRGKVQ
                     AYYKERAGFLPEEKKFDERSEPDDFQNH"
     gene            complement(1106..2188)
                     /locus_tag="PK125_00980"
     CDS             complement(1106..2188)
                     /locus_tag="PK125_00980"
                     /inference="COORDINATES: protein motif:HMM:NF012993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HPB36710.1"
                     /translation="MVRDYPKYKVAAVQAGPVYLDRPDYFDVNATLAKACTAIEEAGK
                     NGARLVVFPETFLPGYPHFSMGLQTMAEAGEFFHLWKQYLLNSPVVPGPETEKIGAAA
                     KKAGCYVVIGINERDPEYDGRMYNSILFMGPHGEVMGTHRKLNITINELFFHTRGDVT
                     RVKPCDNIRVYDEPLGKLSGLICGEHFQPLLKQHLIVEGEQIHAALWPGLAMIKTVML
                     VMTQALCVSARCWAVLAAPYIAKEAVPKDTYPNNHFHQSIGGSCVIDPFGNIVAGPLY
                     EEEGILYHDVDLGLIPIGKAESNLVGIYDRRDVLALARREQPFRPVVPMEWVREAAPQ
                     PNDEQIAALNKRLAKLESKEDDAAGN"
     gene            complement(2570..3370)
                     /locus_tag="PK125_00985"
     CDS             complement(2570..3370)
                     /locus_tag="PK125_00985"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HPB36711.1"
                     /translation="METLEMLQKKQTPLDCAIVDIRLLDMDRLKLLKVIEVDYPNLPV
                     IIITGHGGPAIEEAARAQNADGYLEKPFAVEEFTRLPAEIPAPAAKAETAKAEAVAPA
                     QSIGAYALVTVDTAANLMGVYQKLYFHENVLYCDAVKGDHYIILLLQADSFGTPHMET
                     GVVSPASLPIETQGTLTACDRRMQANMKVFGSKTGMENWEIIADMALKIGAAPGLKPA
                     EDIQKEIRRIVVPYRDRKAGSALCLSISPRTTWTRSRMYRVRIISASR"
     gene            3882..4346
                     /gene="greA"
                     /locus_tag="PK125_00990"
     CDS             3882..4346
                     /gene="greA"
                     /locus_tag="PK125_00990"
                     /inference="COORDINATES: protein motif:HMM:NF001261.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HPB36712.1"
                     /translation="MKFPITREGYESLKKEHEFLLNTKRPAILKAIEEAREHGDISEN
                     AEYDAAKEEFQFLQQRVAEIEEKIMNSEIVDGRQGDGDTVAFGCCIVLKNLDTDEDVI
                     YTLVGPYESDIQKGRISMSSPLGRALLGKGVGDEVAFKAPGGNRIYEIVRIE"
     gene            4518..5396
                     /locus_tag="PK125_00995"
     CDS             4518..5396
                     /locus_tag="PK125_00995"
                     /inference="COORDINATES: protein motif:HMM:NF015724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CsgG/HfaB family protein"
                     /protein_id="HPB36713.1"
                     /translation="MNINRVHVGKILVIWCVSIVFVIAIGIAQCGATEPVLGGSNTTA
                     TGAAGGATAEGASNQLERCAESLGTVAVVEDQSAPWYGYLYSNYKLGSTVPVLRMMIQ
                     QSNCFVVVERGRAMSNVMQERALEGSGELRQGSAFHKGQMVAADYTMSPSITFSQKGT
                     SGIGGALGGLMGSAGRVFGAVAGGLKSNEASTTLILVDNRSSVQLAAAEGSAKNWDFN
                     LAGGLLGSTAGGGLGGYTNTPEGKILTAAFMDSYNKLVRAVRNYRAQTVQGGLGTGGR
                     LGVQGGQTPASQGIGR"
     gene            5838..6287
                     /locus_tag="PK125_01000"
     CDS             5838..6287
                     /locus_tag="PK125_01000"
                     /inference="COORDINATES: protein motif:HMM:NF024498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin fold domain-containing protein"
                     /protein_id="HPB36714.1"
                     /translation="MGAVTYPDLKVVEFVNEALVPLQLQADSDLAEDFTVRWTPTLII
                     LDQNGKEHSRTVGYIAPEEFVPAMLLGMAKMHFDLRQFDSMAQCLEKIINVYPWSLAA
                     PEAIYYHGVCGYKTTHNAEPLKLAYERIAREYPYNEWVKRALPYRLL"
     gene            complement(6321..6599)
                     /locus_tag="PK125_01005"
     CDS             complement(6321..6599)
                     /locus_tag="PK125_01005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB36715.1"
                     /translation="MYSIGWYSGNPSLCLPRNKKVQQVHVNLQRRLLGTSSSQAHDHR
                     FNGEVTSVGQTAKALTIKDKPAEKTFDGANVKIEKVPDTGGKVYGERL"
     gene            6848..7525
                     /locus_tag="PK125_01010"
     CDS             6848..7525
                     /locus_tag="PK125_01010"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HPB36716.1"
                     /translation="MRILLVEDDIKIVSFIAKGLKAVGYAVDHAADGEEGLSLALTEP
                     YDTAIVDIMLPKRDGLSLIEELRKRKIHTPVIILSAKDSVDDRVKGLQTGGDDYLTKP
                     FAFSELLARVQALIRRASGVSEPTTLTTGDLSMNLLTREVRRKNRHIELQPMEFSLLE
                     YLMRNAGRVVSKTMIMEHVWDYSFDPQTNVVEARICRLRDKIDRDFDRKLIHTIRGVG
                     YALKDTP"
     gene            7503..8927
                     /locus_tag="PK125_01015"
     CDS             7503..8927
                     /locus_tag="PK125_01015"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPB36717.1"
                     /translation="MPLRTRLNPRHSLAFRLTLWYATIFTLSTGIAFALFYIFITSII
                     RDRTDQELIGQARRFSAILSTESPDEVSKAMVIEAQAAGVRKVFFRLLYPDGQAFFSS
                     NMTYWKDITVNLSAIKRLAQGTSYVVETITIPDRGQKRVRVLYAMLGPNVAIQVGESM
                     EGYSRFHDVFEIILIFTMAFLFIVAVGVGWFMAKTAVSGVEAVTRTARRISAGALEER
                     VPVRDSSDEIDLLARTFNEMLDRIQVLVREVKEMADNIAHDLKNPIARIRGTAEVTLT
                     TSRSIGEYENMAASTIEECDRLLDMINTMLMISKTESGVYKPSLQDVDIARLVRDTCE
                     LFSPGAEDKGLTLRCEAPHTILMRGDIHMIQRMISNLLDNAIKYTSTGGKITLDASVD
                     IAGQVTVSVHDTGAGILPDELPHIFERFYRGDQSRSTVGIGLGLSLSRAIARAHGGDV
                     SVTSDFGKGSTFIVTFPNTSPPTS"
     gene            9082..10542
                     /locus_tag="PK125_01020"
     CDS             9082..10542
                     /locus_tag="PK125_01020"
                     /inference="COORDINATES: protein motif:HMM:TIGR02037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegQ family serine endoprotease"
                     /protein_id="HPB36718.1"
                     /translation="MEQMGRFKKVFVTLAILALIVGLGYGVSRAMKDSDKAPSKSTGV
                     PIMVPGNFTEIAENVRNGVVNIQAVKNGKSGGRVFRHFFGNPRGQQNPFEDFFSPKFG
                     WDDPLEGFQQKSLGSGFIIDREGYIVTNNHVVENADEIKVKLASGKEFDAKVVGRDPK
                     TDLALIKIKGGSDLQPLQLGDSDALKIGSWVVAVGSPFGLEQTVTAGIVSAKGRTIGA
                     GPYDNFIQTDASINPGNSGGPLINTKGEVVGINTAIVASGQGIGFAIPANMAKEVIPH
                     LKEKGKVTRGWIGVGIQEMSPELIKSFNLQEKKGALVSQVFKDSPAEKAGIERGDVIL
                     EFDKKEIAVSRDLPRIVAAIPVGKTVTVKIWRNGKVMTKDVKVGEMEEKVAVTAKTQT
                     GKRLGIAIQNITPDIAQALSLKDSNGALVAQVEPGSPAENAGLQRGDIIREVNRKPIK
                     DTNDFMQKIEEAKSTGTILLLIQRDKNSLYLTVVPK"
     gene            complement(10714..11076)
                     /locus_tag="PK125_01025"
     CDS             complement(10714..11076)
                     /locus_tag="PK125_01025"
                     /inference="COORDINATES: protein motif:HMM:NF014035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HPB36719.1"
                     /translation="MTIILLYVLLGLVAGVLSGLIGIGGGVIIVPALVFIFGLSQHQA
                     QGTTLALLIPPIGILAAWTYYKEGYVDLRIASFIAAGFLVGSLVGAKFANTLSNVVLE
                     RVFGVALLLIALKMILSR"
     gene            11304..13820
                     /gene="glgP"
                     /locus_tag="PK125_01030"
     CDS             11304..13820
                     /gene="glgP"
                     /locus_tag="PK125_01030"
                     /inference="COORDINATES: protein motif:HMM:TIGR02094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucan family phosphorylase"
                     /protein_id="HPB36720.1"
                     /translation="MTPVTCKETDGFDSLAELALDLYWYWNHSADEVWELLDPVLWKL
                     TYNPWVVLQTISRDRLHRVLEDPIFCEKIKNLLQSKQEQERPPTWFQRHHANSPLTSV
                     AYFSMEFMLSEALPIYSGGLGNVAGDQLKTASDLGVPMIGIGLLYQQGYFRQIINVDG
                     AQQALFPYNDPGQLPISPLREPDGEWLRLQIKLPGYSVWLRTWQVQVGRVKLFLLDSN
                     DPANFPTRRGITSELYGGGAELRLQQELVLGLGGWRLLKALGINPEVCHLNEGHAAFV
                     VLERARTFMEETGQPFDVALAVTRSGNLFTTHTPVDAGFDRFSPSLIEQYLGGYAQNR
                     LGISLQNLLALGRNDPNDFSEPFNMAYLALRGSGAANGVSRLHGKVSRRIFQGLFPRW
                     PENEVPIEHITNGVHMSSWDSEEADALWTATCGKDRWTGALDTVEGDMLAVSDLRLWE
                     CRSSARQSLVEYVRERYALQMQASGFSEQQVEEARNALDFNTLTLGFGRRFALYKRPN
                     MLLHDPNRLLRILNDKNRPVQLIIAGKAHPADQKGQALIQEWTHFIKRPEVGNRVVFL
                     SDYDMLLTGRLVQGVDVWINTPRRPWEACGTSGMKVLVNGGLNLSELDGWWDEAYTPE
                     VGWAIGDGKEHGDDPGWDAVEADMLYSILEDEVIPEFYLRNQDGIPLGWVNRIRESMA
                     RLTPRFSANRTVREYTEKYYIPGAQAYKTRAVKNGSMGERIVLWQQDVRQKWSHLRFG
                     DMKVETTEGKYIFELQVYLNSLEPDSVSVEIYADGIGGGDALCLPMRRGRRLVGTENG
                     YIYTREVETDRPVSDFTARLIPSFPGVAVPLEAGHILWQK"
     gene            complement(13888..15069)
                     /locus_tag="PK125_01035"
     CDS             complement(13888..15069)
                     /locus_tag="PK125_01035"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPB36721.1"
                     /translation="MNKERLWTKDFIVVSTTNFLIYLVFFLLMVIMASYAVDKYHATT
                     GTGGLVSGIFIIGILIGRLGTGRIIEDVGSRRVLVIGTLLYIITSTLYFAAINLPLLI
                     LMRFLHGFAFGVANTATGTIAAQIIPRGRHGEGIGYYSMSQILATALGPYLGIVLSQR
                     VDFKMIFLIASLVAAISFAISFVVSQPSYKPPKQVQTKTVKQFQISEFLEFRAIPISI
                     IVLIVGFGYSVVLSFLSLYSRQLCLEEAASFFFLIYAIVVIFSRPFSGRLLDARGANF
                     VVYPCILFFSIGMLVLSQATYGITLLLSGAIIGLGYGNFLSCGQAISVKSTSPNRFGL
                     ATATYYMFLDIGFGVGPYLFGSLVPVTGYRGLYFVMAMVILSTTLLYYFLYRKSLHFS
                     R"
     gene            complement(15489..16511)
                     /gene="corA"
                     /locus_tag="PK125_01040"
     CDS             complement(15489..16511)
                     /gene="corA"
                     /locus_tag="PK125_01040"
                     /inference="COORDINATES: protein motif:HMM:TIGR00383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015087 - cobalt ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HPB36722.1"
                     /translation="MMKKEDSDSGTSAELSGITVIDYDEKYFDERDIERIEDLAAYRL
                     TPTVTWINFRGLPDSDNLARIGEIFGIHPLTIEDILNTGQRPKLEDMVDYIYAAVKTF
                     HFHEKSHRMMTAQVSLVITRQAVLSFEEEGGVVFEPIREKLRKYRGRIRRMGTDYLTY
                     RLLDAVVDSYFDMVEKIGDNLELLEDRVVGNPTPDVLKAIHLLRMETTTILRSVWPLR
                     EVVSALTHDEFPMVKRDSVTYFRDIYDHTVQIIETMETNRDIISSLLDIYLSSTSNRM
                     NEIMKVLTVIATIFMPMTFLAGVYGMNFKYFPELEWRWAYVSFWIAVIIIAVLMIFYF
                     RRKKWL"
     gene            complement(17193..17609)
                     /locus_tag="PK125_01045"
     CDS             complement(17193..17609)
                     /locus_tag="PK125_01045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB36723.1"
                     /translation="MKRFLIVTLAVLMGVSLITAVFAQGPTDTAGTSQPSKKVVKKAP
                     AKAKAHEYKGEVVAIDAAAKTLTIKGKTDEKTFDVANVKMKKEPKAGDKVLVKYAEKD
                     GKTIAKSVTIQKAGKKAPAPKEETKPGPSTAPAPAK"
     gene            complement(18115..18405)
                     /locus_tag="PK125_01050"
     CDS             complement(18115..18405)
                     /locus_tag="PK125_01050"
                     /inference="COORDINATES: protein motif:HMM:NF015925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HPB36724.1"
                     /translation="MFIVIINFPPIKDGKDAEFREWFSWSNTEFAKHEGFISRRLLKP
                     LKGGNYAAIVEHKSQETFMAMHTSPAHDEAGKRVQPLFNGNPTPTFYEAIVE"
     gene            complement(18456..19580)
                     /locus_tag="PK125_01055"
     CDS             complement(18456..19580)
                     /locus_tag="PK125_01055"
                     /inference="COORDINATES: protein motif:HMM:NF012766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HPB36725.1"
                     /translation="MDKDTYQARIKRCTEMMKTEGCDLLLLTKPSNMQYLTGDGRLCA
                     YAMITTDAKVAMGVPKTDIEDVKQGAFFHHIVGFEDEVGMIHSIAHYFEHFNLKEGTV
                     GLEFTFLTQSMMGIVTHPHAKPKDVMPKDCTPILSSLRLVKEEREIDLLRQAAVVADI
                     GMKAAIEAVKQGITESQIAAEAEYAMRYAGAEDFWRTYLSSGPRTGIAHGLPTNRKLE
                     TGDLVMIDIHPIVNGYSSDICRTVCVGKPSNGKQVAYNLYLKAQQASIAKAKAGIGMV
                     ELGEAMHGIIRDGGYGEHIFGPPIHGVGIDFEESPLPPGHAFFHGEKEPPPLPVNVVI
                     AIGNCGLYTDSWGVRVEDTVVVGQQEPIVLTRYPYSLEVK"
     gene            complement(20013..21470)
                     /locus_tag="PK125_01060"
     CDS             complement(20013..21470)
                     /locus_tag="PK125_01060"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF016777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HPB36726.1"
                     /translation="MGSNHFSLRVFFPLQILANIPFIRYKQHMRIIDVHIHVGHWYEW
                     TESARTVWMDTGPYVTKIFDKSGNRLSQQYGDVIKDEGVFGGILIPEYSPETNGVLPF
                     EGAAEVHTYHPELIPIASLNPNYHRDPAAMQPEVPALAQREFSHSAVLMGAFEKQLKM
                     GARGLKLHPIHGHFFANDQALYPVYEKCEREGLPVMFHTGNSLLSGSKMRYSDPYTFD
                     DVINDFPDMKVILCHGGRGFWYQIAELLVRSFKNVYIDISGLPPKNLLTYWPSMKKLV
                     HKFLFGTDFPGVPGIKKNYEEARELIKDDTAMELIGFQNAYNLFGFWKEGIFEVKNPN
                     EIFTVIDNGSKRYSGIIPNDCYHEPYMPMNELLTEMERMRFYGYRKDLDLLGVMAKEP
                     IKDTTLIRHAYVLTGQQGKGIGSKLLHFIETQVDTEWLIVGTWKAATWAIDFYKKHGY
                     ELMGNKDELLRKYWDIPERQIETSVVLGKNMKGRR"
     gene            21469..22182
                     /locus_tag="PK125_01065"
     CDS             21469..22182
                     /locus_tag="PK125_01065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB36727.1"
                     /translation="MTTSEMKMDWEYGNVTTMPGNMFFMLGVIHRDKYGPVLLKYWLD
                     RIQPDSITLELSHYGMRFRRNFSEEYTRRIDKIIVNRKEKGEPCNEEVMASLLSYVRM
                     PYEYDVVSDYGTEHSIPFYLIDMDFFSYVKLRNIEDLLSEENIDRMFSFPELQNGNLE
                     MTTARLYFEKGIALTRYGKEMYIRDRYMSTKIQDLMKHSNKKRLVHVCGWQHLQDPSG
                     LYNALNPIKVFFHDKTVCI"
     gene            22160..22777
                     /locus_tag="PK125_01070"
     CDS             22160..22777
                     /locus_tag="PK125_01070"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HPB36728.1"
                     /translation="MIKLFVFDLGNVILPFEHRQIAVKLHEKSTEKEAFTPEQLFDVM
                     FDLQNGLINPYEEGLMSSAEFLSNLKRQFRLDIDMNAFSNIWNPIFRDDPEVNKVIFY
                     LKEKRYPLFLLSNTNELHFSYIMENYPIVHWFDEWLLSFEVGAKKPKQRIYDAIFEKM
                     DVKPNEVFYVDDISKYVETAQQLGLHGVVFRSAAQLWDFIQKNVI"
     gene            22861..23664
                     /gene="lgt"
                     /locus_tag="PK125_01075"
     CDS             22861..23664
                     /gene="lgt"
                     /locus_tag="PK125_01075"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: protein motif:HMM:TIGR00544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HPB36729.1"
                     /translation="MLPYPNIDPVIFRIGPFAMRWYGLMYVFGFVSSYLLSAYQLKKK
                     AFTLTRAQLDDLYFYLILGLIIGARLGYVIFYNLNFYLENPLEVFVLWHGGMSFHGGL
                     IGSFLAGYIFIKRKKLRFFSVADLIIPTCPPGLMFGRIGNFINGELFGKPSDVPWSMI
                     FPQGGNIPRHPSQLYEAFLEGFLLFIILWIYKNHKKREGDVFAAFLVLYGIFRIICEI
                     FRVPDLPVGYILGFLSMGQALSIVMIIVGLLIKFVYLPRAGQHIERLRK"
     gene            complement(23739..24329)
                     /locus_tag="PK125_01080"
     CDS             complement(23739..24329)
                     /locus_tag="PK125_01080"
                     /inference="COORDINATES: protein motif:HMM:NF012696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HPB36730.1"
                     /translation="MAETLNVKQSLKKLLDGNKRCAAGTQQNPRQDAKRRKEVTKGQK
                     PFAVIVGCSDSRIPPELIFDQGLGDLFVVRLAGNIVDSLAMGSIEYAVEHLATKLVVV
                     LGHGNCGAVTAAAKSADAPGHVGSIVKAIQPAIKKARKLKGDLVDNAIRANVSLVVNK
                     IKSSKPIMVEMIEKGDVQVIGAYYNIETGEVELLEG"
     gene            24593..26581
                     /gene="acs"
                     /locus_tag="PK125_01085"
     CDS             24593..26581
                     /gene="acs"
                     /locus_tag="PK125_01085"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF001208.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003987 - acetate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019427 - acetyl-CoA biosynthetic process
                     from acetate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HPB36731.1"
                     /translation="MAEVKEETKYFYPPKEFVEEAYVSSREEYETMWKQSVYEPEEFW
                     GNIASELTWFKKWLKVNREDFSKGQIEWFIGGKTNISYNCLDAQIKKGRGDKVAILFQ
                     GEPEDDVVKLTYKDMLTYVSKFANVLKKRGVRKGDRVAIYLPMIWQLPVAMLACARIG
                     AIHTIVFGGFSAEALRDRILDAGAKVLITANGYWRSGKNVSSKANADIACDLCAKEGH
                     TVDKVIVVKRLDKFDVPMKAGRDTWFEEELAASDISDVCPAEWMDAEDPLFILYTSGS
                     TGKPKGVLHTVGGYMVYVYISFKYIFDYKEKDVFFCTADIGWVTGHSYIVYGPMCNGA
                     TSIVFESVPTFPNPDRFWAIVEKFKVNSFYTAPTAIRALMKEGEKWPQGRDLSSLRVL
                     GSVGEPINPEAWLWYHKYIGGERCPITDTWWQTETGGILICALPGAWPTIPGFATLPF
                     FGVDARVLQARSSAKEPAVDSPVNEQGELCIGRSWPGMMRGVFGDPERFYTTYFVQQP
                     GFYFSGDGAIRNEDGYFRLMGRIDDVVNVSGHRMGTAEVEAALNSLTTAVAESAVVGF
                     PHEVKGEDLYAYIILKSGVEPSDALKKELVAHVRKEIGPIASPGKIQFVSGLPKTRSG
                     KIMRRILRKVAAGAIDELGDTTTLADPAIVDEIVKGRQ"
     gene            26930..27886
                     /locus_tag="PK125_01090"
     CDS             26930..27886
                     /locus_tag="PK125_01090"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025033.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HPB36732.1"
                     /translation="MAKDYTLSSTKSYHIGGLKSLGWELTICNALQQEGTPLRKLLTC
                     NESFGRLLYDCLCTFLPLWNVTHVLEVGGGYGYLMKDFLEKDSSLKACMLDISPFLLN
                     KQRETLKEFSVEYREGDFLEVLPSDLSAFDLVIMNENLGDFPSLINIRTEVFDSKIAN
                     NDPVLKWVQGIFEKYGLERPEGKVFNLNMGAIEALEKLCCAKVPYIYIGEHSCEASVS
                     GEMSSLFQIKSTGNPERITLAGHDEYTIKFSYLQKIASFHGYESIRGPFADFIPLTLT
                     DEARFALMYGGHYSDEAEVMSQFVEDLYKYEYLILKEKEEPI"
     gene            27883..29058
                     /locus_tag="PK125_01095"
     CDS             27883..29058
                     /locus_tag="PK125_01095"
                     /inference="COORDINATES: protein motif:HMM:TIGR02486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reductive dehalogenase"
                     /protein_id="HPB36733.1"
                     /translation="MSGYHERNWEPFPMDSIKRVDRPTTVIHEDQVKRVDERESGFNR
                     AFRGDFGERLSLEVRRFVPKQPVSGALLWMTSNMMRMVDGLVAARHAPLPEDPAAISR
                     HIKELAYFLRADAVGICKLPQYAVYTHKFPKGDPIELNHTYAIAVLVDQDWRTANAFT
                     GSDWISNAMSFLAYSTTGFIACILAEYIRRLGYPARAHHARNYQVVLPPILLWAGLGE
                     MARMGDCVIHPFMGPRLKGAVVTTDLPLLVDKPIDFGLQDFCSKCKKCARECPSGAIS
                     SGDKVMDKGYEHWPLNAERCTRMRVGNKRGSGCGTCLKVCPWNKPYTLFHRGVNWTMR
                     HSALARSLAIKFDDLLGYGKPHYEDKWWFDLEEVDGIIDVPRYSVKDQKDFKQDIDY"
     gene            29266..29958
                     /locus_tag="PK125_01100"
     CDS             29266..29958
                     /locus_tag="PK125_01100"
                     /inference="COORDINATES: protein motif:HMM:NF024122.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase-like hydrolase"
                     /protein_id="HPB36734.1"
                     /translation="MKGKKVAFFDIDKTIYDGYLIFPLAEYFLKENVVGKDVVHSLYN
                     DLHLYRSQQVDYETTVENFNIHLANGLKHRDTDSILALTKSFLETDEGNKFFPFAADL
                     MELLEPTYDIYFVTGEVQCVGKAVAEYFAVHGYVSTEMDIKDSVYTGTIRKSLARKEG
                     KRDAINHVIHKYPHEGSLAFGDSEGDIEMLDAVAQAFCINATEGLREVALSKGWHIAK
                     PETILDAVKKTL"
     gene            complement(30233..31450)
                     /gene="dinB"
                     /locus_tag="PK125_01105"
     CDS             complement(30233..31450)
                     /gene="dinB"
                     /locus_tag="PK125_01105"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF002677.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HPB36735.1"
                     /translation="MSRIFLCVDMDAFFASVEQHDNPDLKGKPIAVTGVGERTVITTS
                     SYEARQYGVKTGMTPREAKKLCPHIIFVTGRNRRYAETCEKLETICDRFTPDRETYSI
                     DEIFLDITGSHHLFGGPEEMARAIKETVKEELGINCTIGIGPNIPIAKLASDLAKPNG
                     LKWIKKEMVASVLETLPVKKLWGIGPHTEEKLRAMGIKTCGELGTAPLSLLIKKFGVF
                     GSQLKAMGSGILDRPLEPADSDPKSIGHSRTFEKDLWKREDIEPQLLSLSEMVGRRAR
                     RYGYKGKKVTLTVRYADFKTFTRQTTIPDCTNDTGEIYRTALSLFDGIHLTTSIRLLG
                     VSLSSFGKNEQLTLFEEPHRKDRDALLKAMDRVNDKFGENKITWASTVNQKKGHSVIS
                     PAWRTSGVRKSDV"
     gene            complement(31616..33670)
                     /locus_tag="PK125_01110"
     CDS             complement(31616..33670)
                     /locus_tag="PK125_01110"
                     /inference="COORDINATES: protein motif:HMM:NF024938.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HPB36736.1"
                     /translation="MNEFFFPNSIAIFGISSSPGNLGIGILENLERFSFKGDVYLIGE
                     KGGSVRGKTILKGIAEVPGIPELAIFIIPAKGIPGKLEECGKKGIRHVIIESGGFSEF
                     GKEKEALEKEVLAIAKQWGIRILGPNCLGTVNLENGLVLPFVRFHETFLQKGDISLIS
                     QSGGLLHDILLLLSCDNLGINKLVSAGNKLMLDECDFLEYFASDPATKTIGLYLEDIK
                     DGRRLFRLATSTNKPVILLKANESSGSSQIAQFHTASLAGDDLIADNILCQAGIHRVR
                     TLDEMISTIKIFSLPLMRGQRLAVIARSGGHAVMAADAVYRHGFRLAHLSQDIFDLVG
                     QKKRVDVIRATNPVDLGDIFDINFHYTILEKTLKENGVDGVLFIHSYDLQTDGDATLA
                     LIQKINAITRVQPKPVVFCMLSEKEQWFNMKDGASFPVFSEADQAVKALARSLSHFRR
                     AAATLRASAVKRRKCLVRTNVSPHTKTSFLSPAKAFELIRQYDLPVADYEVTRNFEEC
                     RNAAQRLGYPVALKTALSGILHKTEKGGVRLHVASDSSLKDAFNSMEPGPYLVQKMVQ
                     TGLELIIGSKLDPAFGHVLIFGMGGIYVDLFKDIAVRMVPVNKKTALRMITDMQGSAI
                     LRGYRGNPPYDLKAITNCISRISKLLIDHPEIVNIDINPLIAYEEGKGCIIVDAKIET
                     AL"
     gene            complement(33845..35080)
                     /locus_tag="PK125_01115"
     CDS             complement(33845..35080)
                     /locus_tag="PK125_01115"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OFA family MFS transporter"
                     /protein_id="HPB36737.1"
                     /translation="MSDDSKVFGMSASAGRWIFVILGMVVNFCLGAVYAYSVFKKPLE
                     ELFNISATQGGLPYMIFLAMFALFTFVAGKFIDKLGPRKVMMVGGAIVGIGWMLTYFA
                     SSIWMVVLTYGIIGGAGVGIVYGGPVSVATRWFPDKKGLAVGLALAGFGGSAIATAPI
                     ASALIGKCGPLGAFGVLGIAFFFITIILSMPMKFPPAGYKPAGWNPPTTATGGAASSF
                     TAGQMMKTGSFYGLWLCYILGATAGLMAIGISAAVGREVIGLEKGAAATLVMIFAVFN
                     GIGRPIFGALTDKITPKWSAILSFVLIFVASALMLAAGQGSVGLYAVAFCIFWLCLGG
                     WLAIGPAATAGFFGLEGYAQKYGVVFSSYGLGAIIGGIISGSAKDAFGSYTIAFYPTA
                     GMAILGIIIALILLKPPKK"
     gene            35510..35884
                     /gene="rpsL"
                     /locus_tag="PK125_01120"
     CDS             35510..35884
                     /gene="rpsL"
                     /locus_tag="PK125_01120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000246374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HPB36738.1"
                     /translation="MPTINQLVRLGREVVKKKSSSPALLNCPQKRGVCVRVYTTTPKK
                     PNSALRKVARVRLTNGIEVTTYIPGIGHNLQEHSVVLIRGGRVKDLPGVRYHIIRGSL
                     DASGVANRKKSRSKYGAKKPKQ"
     gene            35909..36379
                     /gene="rpsG"
                     /locus_tag="PK125_01125"
     CDS             35909..36379
                     /gene="rpsG"
                     /locus_tag="PK125_01125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013054923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HPB36739.1"
                     /translation="MPRKGHVSKREKLPDPKYNDLVVARLINRVMLDGKKSTASRIVY
                     EAFDLIENKLKEEALKVFHRAMDNIKPELEVKARRVGGATYQVPVEVRLNRKLSLAIR
                     WIMRYAKERSERTMTEKLAGEIIDAYNNKGGSVKKREDTHKMAEANKAFAHYRW"
     gene            36387..38468
                     /gene="fusA"
                     /locus_tag="PK125_01130"
     CDS             36387..38468
                     /gene="fusA"
                     /locus_tag="PK125_01130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018003270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HPB36740.1"
                     /translation="MNQIVRNVGIMAHIDAGKTTTTERMLFYTGVNNRIGEVDDGNAT
                     MDWMEQEKERGITITAAATTCSWKGHRINIIDTPGHIDFTVEVGRSLRVLDGAVALFS
                     GVEGVEPQSETVWRQADRYRVPRIAFVNKMDRPAADFHRCVRMIKDILKANPLILQIP
                     VKDGDELLGNVDIITQRATYYDNATRGIEYTVKDIPAHLKDEALVLRERLMESLAEID
                     DGFMTAYLDGALIEETEIKRIIRKGTLSGAILPVLCGSSFKNKGVQPLLDAIVDYLPS
                     PADVSPYEYWTIEGEEGYLKGTPDEPFSGLVFKIMNDPFVGQLSYMRIYSGELKTGET
                     VLNNSKGRTERIGRIIRLHANKREEVKKIQAGDICAIVGLKGASTGDTLTGQDFEILF
                     ETIEVPAPVVSIAIAPKKKDDLKKMWLVFNRYTVEDPSLNVKLDSDTGEVILSGMGEL
                     HLDIIMDRARREHNLDMLSSPPQVAYRETITKSVTGIGKYIKQSGGKGQYGHVVLHVS
                     PIEGADFVFENKIVGGVIPREYISSVEAGVKERMEKGVLFGYPLVNIKVELIDGSYHE
                     VDSSELAFKLAGSLGLKDGVTKANPVILEPIMKVDINVPGDCLGPVIGDLSSRRGRIA
                     ELGDRNDFKYIVAHIPLDEMFGYTTHLRSATQGRGYYTMEFFHYTPVPQHILENLMKN
                     REKTVTEALYG"
BASE COUNT        10298 a         8698 c         9122 g        10384 t
ORIGIN      
        1 ctcctgttct gatcgtcgtc tcctctttcg atggtgtggt tgtttccgta ttttcccttg
       61 gtacccgaga ggccgtaacc agagccgcag ggtctcttgg ctgaccagaa gatgcgcctc
      121 ctcttccagg gtctttcgca agatgagaga aattaaagtc cttctatttt tctttcacca
      181 gactgatcac gtggttcttc cagggcaaga cccgtgatga gggtcgtcct ctatttccgt
      241 ggatgagtcc acatggagcg ttctgtgcca ccttcttctc catcctctgc acctgcctct
      301 ttgattggtc atcctccctt caatgggagt ttcgattact cactttccaa cgtcttttct
      361 tagaccattt cctccggttt gctcacgtac ttctgcagct tcgaatggag ctcgtgtctc
      421 tcaatggttt tggaaatcat ccggctcact tcgctcatca aactttttct cctccggtag
      481 aaagccggcc ctttccttgt agtacgcctg caccttgcct cgtttcttcc cacccactat
      541 gatgggataa gaataggacc tggtggcctt ttccttgccc attgggcttt gcgagtgttc
      601 gacgttgtcc ggccccatat agaggcatag gcaatccacg gaaactggag gctctttatc
      661 aggtggctgg ttgtattatt gagcgtcgtt ccgaaaaaac cgtctgactc gattatcagg
      721 cccaatgctg taaagtcact ccaattcctg gacccacttc ttcaaatcaa gttctatcga
      781 cgccatgggt tgggcttgct gcatcgcctt cctccgccgt aaacatcccc tgcattattg
      841 tatacggtat agcacatatc gtttccctgt gcctttatgc cctgtctttt cgccttcacc
      901 ctcgttcccc gaagaacaag tcaattccat tttgacgatc cgggtctggt aggtttttcg
      961 gtggattcag tttttcaata caagaaatcc tcatgaacca aatggtcaat cgtccccgga
     1021 ttgtggttat tttgagcagg cgattggggt tgtacgttgg tacgtcattt ctcctagtgt
     1081 ggtccccttg tactacccca atcatctaat tgccggctgc atcatcctct tttgactcca
     1141 gttttgcgag gcgcttattc aaggcagcaa tctgctcatc attcggctgc ggtgctgcct
     1201 ctctcaccca ttccatgggt acaaccggac gaaacggttg ttcccggcgt gccagcgcca
     1261 gaacatccct tctgtcgtaa atgcccacta aattcgattc agccttgccg atgggaataa
     1321 ggccaaggtc cacgtcatgg tagagtatac cctcttcttc gtacagcggc ccggccacta
     1381 tattaccgaa ggggtcgatc acgcagctgc cgccgatgct ctgatgaaag tggttgttgg
     1441 gatacgtatc tttgggaaca gcctccttgg caatgtaggg cgctgccaac accgcccaac
     1501 accgagcgga aacacagagc gcctgggtca taactaacat aaccgtttta atcattgcga
     1561 gtcccggcca gagggcagca tggatctgtt ccccttccac aatcaaatgt tgcttgagaa
     1621 gaggttgaaa atgctcacca cagatcagtc cgctgagctt gcccaggggt tcgtcgtata
     1681 ccctgatgtt gtcgcacggc ttgacccgtg tcacgtcacc gcgagtatga aagaaaagct
     1741 cattgattgt gatgttcagt tttctgtgag tccccataac ctcgccatgg ggccccatga
     1801 acagaattga attatacata cgcccgtcat actcggggtc cctctcatta atgcctataa
     1861 cgacgtagca gccggccttc ttagcagcgg cacctatctt ctcggtttcc ggacccggca
     1921 ccacaggaga attaagcaag tattgtttcc acaagtggaa aaattctccg gcctcggcca
     1981 tggtctgtag gcccatggaa aaatgaggat agccgggcag aaacgtctcc ggaaagacta
     2041 ccaatctcgc accattcttc ccggcttctt caatcgcagt acaggccttg gccagcgtgg
     2101 cgttaacatc gaaataatcc ggcctgtcaa ggtagaccgg accggcctgt acagcggcaa
     2161 ccttgtattt cggataatcc ctaaccatgg tacaccccct tggtttatga tttacttctt
     2221 agacctgttg cgcctcgctg ccgcataggc ggccgttccg cctaaagtga taggatcaag
     2281 caaagtccgc ctccactttc atctcgtacc catcaaccat tacacgcttc accgtaacac
     2341 cctgtggttg aaaacagata aggagactcg gcttgacgat cctcttcctg cgacaggccc
     2401 acttctactg gatagcacag gcaatcatgc tttgcaagga aaaatattga caaatttttg
     2461 gagtgtcaac gcaaagcgga aatgtcctcg ctccttctac ctttcgactt cgcaatgttc
     2521 caatctgcaa cctgtctttt ctgcctgccc tattctgcca gtctgctctt caccttgagg
     2581 cagaaataat tctcactcga tacatacggc ttcttgtcca cgttgtacgg ggagagatcg
     2641 acaggcagag cgctgaacct gccttcctgt ccctgtacgg cacgactatt ctgcgtattt
     2701 ccttttgaat gtcttccgct ggcttgaggc ccggggccgc acctattttc aatgccatgt
     2761 ctgcgatgat ctcccagttc tccatgcccg tcttggaacc gaacaccttc atattagcct
     2821 gcatccgacg gtcacacgcc gtaagcgtgc cttgcgtctc gatagggagc gaagcgggcg
     2881 aaaccacgcc agtttccatg tgaggcgtcc cgaacgaatc ggcttgcagc aacagaatga
     2941 tgtaatggtc acccttgacg gcatcgcagt aaagcacgtt ctcgtggaag tagagcttct
     3001 gatagacacc catcaggttg gcggcggtat caacggtcac cagcgcgtat gcgccgattg
     3061 actgggcagg agcaactgct tcggccttgg cggtttcagc ctttgcagcc ggagcaggta
     3121 tctcagcagg gagccgggtg aactcttcca ccgcgaacgg cttctccaga tatccatctg
     3181 cgttctgagc ccgggctgcc tcttcaatgg ccgggcctcc atggcctgtg atgatgatca
     3241 cgggcaggtt cggataatcg acctcgatca ccttcaagag cttcagccta tccatgtcca
     3301 gaagtcggat atcaacgatg gcgcagtcga gaggggtctg cttcttctga agcatttcga
     3361 gcgtttccaa cccctcatac cgttttcgca gggttcggtg tcgtacccct tctgcatcag
     3421 gctgagcgcc acatgccgta tcaacgatgg ctcatcgtcg atcagtaaca ctttctttcc
     3481 atggccgttc ctctatttcc tatttatccc attttcggtg ttttcatgtt tacgcctgcc
     3541 tcgtatcttc ctgacgactg atagagcaag gcggatgcca tagtggaaag attcttaatt
     3601 acagtgcctc actcattatg aggtggtttt tgatccccct tgcggcgata agaaatatat
     3661 acataggtat ctaagtatct gttatgtatg atgattctca agcgttacgt tttgttacat
     3721 tgacagaaag tggtaattgc atagagcggc agggttttca attgtaaaaa atgttgcgga
     3781 taaaacgtta gcagctgatt tgcagggtgt tataccacaa tgaggttgat aatttggaat
     3841 acatgtagta tcttgtacac catacaaatt tcggagcgtt tatgaagttt cccattacaa
     3901 gagaaggtta cgagagctta aaaaaagagc atgaatttct tttgaacacg aagagaccgg
     3961 caatcttgaa agcaatagaa gaagcgcggg aacatggtga tatctctgaa aatgcagagt
     4021 atgatgcagc gaaagaagaa tttcagtttc ttcaacaaag ggtggcagaa attgaagaaa
     4081 agatcatgaa ctcagagatt gttgatggaa ggcagggaga tggtgatact gtcgcttttg
     4141 gctgctgcat agttctgaag aaccttgaca ctgacgaaga tgttatctat acattggtag
     4201 gcccatacga atctgatatt caaaaaggcc gtatctccat gagttcgccc cttggcaggg
     4261 cccttctcgg aaaaggagtg ggggatgaag tagcattcaa ggctcccggc ggtaaccgaa
     4321 tctacgaaat agtaagaatc gaataaacac gatttaaaaa agatgatttg ggctttggtt
     4381 gttcacacct tgcagtgatg attgatgtca tttgatcgca aacactcaca atccagcacc
     4441 caatattgac tatcatgctt aacaagagta ttattactgc gataaataga gcagaaaaaa
     4501 attaggagga gtcaaggatg aatataaatc gggtacatgt gggcaaaatc ttggttatat
     4561 ggtgtgtgag tatcgttttt gtgatcgcaa ttgggatagc ccaatgcggt gctacagagc
     4621 cggtccttgg tggttcaaat accacagcaa ctggagcggc aggaggagcc acagcagagg
     4681 gagccagcaa ccagctcgag agatgcgctg agtctcttgg tacagtagct gttgtggaag
     4741 accagagtgc gccttggtac gggtacttgt attcaaatta caaacttggt tcaactgtgc
     4801 cagtgctgcg catgatgatt cagcagtcaa actgttttgt ggttgttgag cgtggtcgtg
     4861 cgatgagcaa tgttatgcag gaacgggctc tggagggtag cggtgagctc cgacagggaa
     4921 gtgcctttca taaaggacag atggttgctg ctgattacac tatgagccca tcaataacat
     4981 tcagccagaa aggaaccagt ggcataggag gcgccctggg agggcttatg ggatctgccg
     5041 gaagggtttt tggcgcagta gctggtggcc tgaagtccaa cgaggcatcc acgacactca
     5101 tattggttga taaccggtcg agtgttcagc tggctgctgc tgaaggaagt gccaagaact
     5161 gggattttaa cctggcaggc ggtctgctcg gtagcactgc tggcggcggt ctgggcggtt
     5221 acaccaatac accggaaggg aaaattctta cagccgcttt catggattcc tacaacaagc
     5281 ttgtgagggc agtgcgcaac tacagggcac aaactgttca gggtggcctt ggaacaggtg
     5341 gccgtctcgg tgttcaagga gggcagacac cggcatccca gggaatcgga cgttaaacaa
     5401 ctctttattt tttagtcaaa tatgcgctgc tgctgtggcc tcaaaggtca ccaggcagcg
     5461 ctcaattatt agtttttctc gttgaaacta ttgatcaaac tctgtcagag tcaagagaac
     5521 cactatcctt cttttccggg tgtccggcac tgatggtatt tgtatcaact accgagcaat
     5581 ttgaagcaaa ggggttattc aaaatagctc aatcaaacaa agaacagcag aatatagatt
     5641 gagtgtcatt tttccttgtg attttggaaa ttagtaacta tattggttgt tacaaaaatg
     5701 aatccttaag ggggtgcgtt atggcaaaga catttgtttg ggaaaatgaa atgaagagcg
     5761 ggatagaacg ctctgttaaa gaaaaaaagc ccatattact tgactttttt tctcctggct
     5821 gaattggatg ctaccagatg ggtgcggtta cgtatccaga tttgaaagtt gttgagtttg
     5881 tcaatgaggc tctggttccg ctacaacttc aggcagattc tgacctggct gaagatttta
     5941 cagtgcggtg gaccccgacg ttgattatac ttgaccaaaa cggcaaagaa cactccagga
     6001 cagttggcta tattgccccg gaggaatttg ttcctgccat gctactgggc atggcaaaaa
     6061 tgcattttga tctccggcag tttgattcga tggcacagtg tctggagaag atcataaatg
     6121 tatatccatg gagccttgct gcgccggagg ccatttacta ccatggtgtt tgtggatata
     6181 agacaaccca caatgctgaa ccactgaagc tggcatatga acgtatcgca cgggaatatc
     6241 cttataacga gtgggttaaa cgagcacttc cttatagact gttatgatgg caggggtgtc
     6301 cttcgctggg caggttgcat ttacaaacgt tcaccgtaaa ccttgccacc agtgtcgggc
     6361 accttctcaa tctttacatt tgctccatcg aaggtcttct ccgctggttt atccttgatg
     6421 gtcagagcct ttgcagtctg accgacagag gtgacttcgc cattgaacct gtggtcgtgt
     6481 gcctgcgagg aggacgttcc caacaatctt ctttgaaggt tgacatgtac ctgctgtacc
     6541 tttttgtttc tgggtaaaca gagtgatgga tttccagaat accaaccgat tgaatacatc
     6601 aagcacacct acgacaaaac ccccggcttc agaattaaca cttcaaatat aacaggataa
     6661 gggattgtta ataccccagc ttttcgggaa gttgcagagg atattgcgct aaagtttaac
     6721 ctctactgaa gtgacatctt gcaagagcgg tgcagccgtc ctgttcgtat tttttatgaa
     6781 ggttgcatat atttaccggt gacactattt tatagacagg tttttgtgtc agaaggattt
     6841 attgataatg cgcatactac tcgttgaaga tgacatcaaa atagtatcct tcatcgcgaa
     6901 aggtctaaaa gcagtcggtt atgctgtgga tcatgcggcc gatggcgagg aaggactcag
     6961 ccttgccctg acagaaccat acgatacagc aattgttgat attatgctcc caaaacgcga
     7021 tgggttatcc cttattgaag aattacggaa aagaaagatt catacaccag ttattattct
     7081 gagtgcgaaa gactctgtag atgaccgagt gaagggcctc cagaccggtg gagatgacta
     7141 tctcaccaaa ccctttgcat tttctgagct ccttgcccgg gtacaggctc ttatccggcg
     7201 tgccagtgga gtatcagagc caaccactct tactaccggt gatctttcca tgaatcttct
     7261 tacgcgcgaa gtcagaagaa agaataggca tattgaactc cagccgatgg aattttcctt
     7321 gcttgaatat ctgatgcgaa atgcaggacg agtggtttca aagaccatga tcatggagca
     7381 cgtatgggat tacagttttg atccccaaac caatgtggtc gaagcaagga tatgcagact
     7441 acgagataag atcgacagag attttgatcg aaaactaatc catacaatca ggggggtagg
     7501 atatgccctt aaggacacgc cttaacccca gacattctct tgcttttcgt ttgaccctct
     7561 ggtatgcaac aatttttaca ttgtccactg gcattgcctt tgcccttttt tatattttca
     7621 tcacctccat catacgtgac aggactgatc aggaactgat cggacaggca aggaggtttt
     7681 cagcaatatt gtccactgaa agtccggatg aggtaagcaa agccatggtt atcgaagctc
     7741 aggcagcggg tgtaaggaag gtattcttcc gcctccttta tcctgatggt caggcttttt
     7801 tttcttccaa catgacatac tggaaggata tcactgtcaa tttaagtgca attaaacgac
     7861 tggctcaggg aaccagctat gtcgttgaaa cgattacgat ccctgaccgc ggtcagaaga
     7921 gggtacgggt tctttacgca atgcttggtc caaacgtagc tattcaggta ggggagtcta
     7981 tggaaggcta ctcgcgattt catgatgtgt ttgagatcat tctgattttc accatggctt
     8041 ttttattcat tgtagctgtg ggggttggtt ggtttatggc gaaaacagca gtgtcgggag
     8101 tggaggcagt gaccagaact gcgaggagaa tatctgcagg agcccttgag gaaagggttc
     8161 cggtcagaga ttcatccgac gagatagact tacttgcaag aacattcaat gaaatgcttg
     8221 atcgaatcca ggttttagtg agagaggtta aagaaatggc tgataacatc gcccatgacc
     8281 tgaaaaatcc tattgcaaga ataaggggta ctgctgaggt aacactgaca accagccgct
     8341 cgattggaga gtatgaaaat atggctgcca gcacaataga agagtgtgac cgccttctcg
     8401 acatgattaa cactatgctt atgatctcta aaactgagtc aggcgtatac aagccctctc
     8461 ttcaagatgt ggatatagca cgccttgtca gagatacctg tgagttattc tcacccggtg
     8521 cagaagacaa aggtttaacg ctccgctgtg aggcccccca tacaattttg atgagaggtg
     8581 atatccacat gattcagcga atgatctcaa acctccttga caatgcgatt aaatacacat
     8641 ctacaggtgg caaaattact cttgatgctt cagtggatat tgccgggcag gttacagtat
     8701 ctgtgcatga tactggtgct ggtatattac ctgatgaact tcctcatatt tttgaacgtt
     8761 tttacagagg agatcagagc agatcaacag tgggtatagg ccttggcctt agtctgtcca
     8821 gggcaattgc ccgtgcccac ggcggtgacg taagtgttac gagtgatttt ggcaagggaa
     8881 gcacctttat tgttaccttt cccaacacca gcccccccac atcttagaac agaagaaacc
     8941 aggaggctgt tattttatcg tctccaaggt tagtacttgc cacattacat tttgataatc
     9001 ttcagatcat attcctgtaa tctcagcatt ctatactatg cccgtaggtg gttaaagatc
     9061 aacaaaacaa ggagcaagaa tatggaacag atgggaagat ttaaaaaggt ttttgtaacg
     9121 ctggcaatcc tggctctcat agttgggctt ggatatgggg tttcgcgggc gatgaaagat
     9181 tctgataaag caccatcgaa atcaaccggt gttccgatca tggtgccggg aaacttcaca
     9241 gagattgcgg agaatgtccg caatggcgtt gtgaacatac aggcagtcaa aaacgggaag
     9301 agcgggggca gggtttttcg tcatttcttt ggcaaccccc gtgggcaaca gaaccctttt
     9361 gaagactttt ttagtccaaa atttggttgg gatgatcctt tagaagggtt ccagcagaag
     9421 agccttggtt ctgggttcat cattgatcgg gagggttata ttgtaacgaa caaccatgtc
     9481 gttgaaaatg cggacgaaat caaggtgaag cttgcgagtg gaaaagaatt cgacgcaaag
     9541 gtggtgggcc gtgatcccaa aacagatctt gctctcatca agattaaggg tggttcagac
     9601 ctgcaaccct tgcagttagg cgattctgac gcgttgaaga ttggaagttg ggtagttgct
     9661 gtcggcagtc cctttggttt ggaacagaca gtaacagcag gtattgtcag tgccaagggg
     9721 agaaccatag gtgcaggtcc gtatgataac ttcatacaga cggatgcatc cataaatcct
     9781 ggtaatagtg gaggtcctct catcaataca aagggtgaag ttgttggaat caacacggca
     9841 atcgttgcat ccgggcaagg tatagggttt gcaatccccg caaatatggc aaaagaagtg
     9901 ataccccacc tgaaggaaaa ggggaaggta acacgtggat ggataggtgt tggaatccag
     9961 gagatgtcac ctgaacttat aaagtcgttt aaccttcaag aaaagaaagg tgctcttgta
    10021 tcccaggttt tcaaagatag tccagcagaa aaagccggga ttgagagggg tgacgtgata
    10081 cttgaatttg ataagaaaga gatcgcagtg tcaagagatc taccgaggat cgtggctgct
    10141 atacctgttg gaaagactgt tacggtaaag atatggagaa atggcaaggt gatgaccaaa
    10201 gatgtgaagg tgggcgagat ggaagaaaag gttgctgtca ctgcaaagac tcaaacaggt
    10261 aaaagacttg ggattgccat tcagaacatt accccggaca ttgcacaagc cttgtcccta
    10321 aaagacagta atggcgctct tgtagcacag gttgaaccag gcagtccggc tgaaaatgct
    10381 ggccttcagc gtggtgatat tattcgcgag gtaaacagaa aaccgattaa ggataccaat
    10441 gattttatgc agaagattga agaagccaag agcacaggga ccatactcct gcttatccaa
    10501 agagacaaaa acagcctgta cctgacggtg gttccgaaat agaactggac ctactggagt
    10561 gtccggccgg tggagattca ccaaccctcc atcccccccc aagcccggcc gggcactctc
    10621 ccatctcttc tataagggct tcaaccttgt ctcatctatg ttgctgaagt cttgaaggtt
    10681 gttttggcgg atttgtttta agggcctcac atactatctg gatagtatca ttttgagcgc
    10741 tataagaagc agagccacac caaacaccct ttccaaaacc acattcgaca gtgtattggc
    10801 aaactttgca ccaaccaaac tgcccactaa aaatcctgct gctatgaagg aggctattct
    10861 gaggtccaca taaccttcct tatagtacgt ccatgctgca agtatcccaa ttggcggtat
    10921 tagaagagca agtgtggtgc cctgcgcttg gtgttgtgag agcccaaata tgaaaaccaa
    10981 ggcaggtaca attataacgc ctccgccgat acctattaat ccactcaaaa cccctgccac
    11041 gaggcctaag aggacgtaga gaagaataat ggtcattcgg taacctccta aaatgttgtt
    11101 tactcatgaa aataagagat atttttacct ttatacatgg ctatcataac ataaatatat
    11161 ggttaaccag tcaaaacatt aaacatgatt ataccagagg atctccactg ttacagccca
    11221 ttgcaacctg ttgattcctg agaaaaacat gtttatgata ttctcattat gagtctgtta
    11281 cagataaggc tggatatgtt gcaatgacac cggttacttg taaggagaca gatggatttg
    11341 attccctcgc agaattagcg ctcgatcttt attggtattg gaatcattca gccgatgaag
    11401 tgtgggagct gctcgacccg gttctctgga agctcactta caacccttgg gtagtgctcc
    11461 agactatctc aagagatcga ctgcaccgtg tcttggaaga tcccattttt tgtgaaaaga
    11521 taaaaaacct gttgcagtcc aagcaagaac aggaaaggcc acccacatgg tttcagcgtc
    11581 accacgccaa ttctcctcta acgtctgttg cgtattttag tatggagttc atgttgagcg
    11641 aagcccttcc gatctattca gggggtctag gcaatgtggc aggggaccaa ctaaaaactg
    11701 caagcgatct cggtgtaccg atgatcggaa taggtctact gtaccagcaa ggatattttc
    11761 gacagattat caatgtagat ggtgctcagc aggctttgtt tccatacaat gatccaggtc
    11821 agttgccgat ttctcctctt cgcgaacctg atggagaatg gctgcgtctt caaatcaaat
    11881 tgccgggtta ctcggtttgg cttcgcacct ggcaggttca ggtcggaaga gtcaagctct
    11941 ttctactcga cagcaatgat ccggcaaact tcccgacccg ccgcggcata accagcgagc
    12001 tttacggggg aggggctgag cttcgccttc agcaagagct tgttctcggc cttggcggct
    12061 ggcggctgct taaagcgtta ggaatcaatc ctgaagtctg tcatctgaat gaaggtcacg
    12121 ctgcttttgt ggttttggag cgagcacgaa cattcatgga agaaacagga cagccttttg
    12181 acgttgctct ggctgttacc agatcaggaa acctttttac aacgcacact cctgttgatg
    12241 cgggttttga tcgtttttcc ccatccttga tcgaacaata tctgggcggg tatgcccaaa
    12301 ataggctagg catcagcctt caaaatctgc ttgcactggg ccggaatgac cctaatgatt
    12361 tctcagagcc attcaatatg gcctatctgg cgcttagggg gagtggggct gcaaatggtg
    12421 tgagccgctt gcatggaaag gtgagcagaa ggatatttca ggggttgttt ccccgttggc
    12481 ctgaaaatga agtaccaatc gaacatatta ccaatggggt acacatgtcg agctgggatt
    12541 ctgaggaggc agatgcgcta tggacagcaa cgtgtggtaa agatcgatgg acaggggcac
    12601 tcgacactgt ggagggagac atgcttgctg tctctgattt gagactctgg gagtgccgtt
    12661 cttctgcccg gcagtccctg gttgaatacg ttcgggaaag atatgccctt caaatgcaag
    12721 cctcgggttt ttcagaacag caagttgaag aagcaaggaa cgctcttgat ttcaacacat
    12781 tgactctcgg tttcgggcgt cgttttgcac tttataagag gcccaatatg cttcttcatg
    12841 atccgaatag acttcttcgt attcttaacg ataagaatcg tcctgttcaa ctcataattg
    12901 ctggtaaggc acaccctgca gaccagaaag ggcaggccct gattcaagag tggacccact
    12961 tcattaaaag acctgaagtg ggcaatcgcg tagtgtttct cagtgactat gatatgctat
    13021 tgacaggacg attggtgcag ggagttgatg tatggatcaa tactccccga cgaccatggg
    13081 aggcatgtgg aacaagcggt atgaaagttc tggtaaatgg ggggttgaac ctgtctgaac
    13141 tggatggctg gtgggatgag gcgtacacac ctgaggtagg gtgggctata ggcgatggca
    13201 aagaacacgg agatgatccg ggctgggacg cagtcgaggc tgatatgctc tactccatac
    13261 tggaagatga ggtaattccg gagttctacc tgcgaaacca ggatggtatc cctcttggct
    13321 gggtgaatcg catacgggaa agtatggcaa ggctcacacc tcgtttttct gccaaccgca
    13381 ctgtccgaga gtatactgaa aaatattaca tcccaggtgc acaggcatac aaaacccgtg
    13441 ctgttaagaa tggttcaatg ggcgaaagga tagtcctctg gcagcaggac gtaaggcaga
    13501 aatggtcaca tcttcgcttc ggagatatga aggtcgaaac aaccgaggga aagtatattt
    13561 ttgaactaca ggtctatctc aatagtctcg aacctgactc tgtttcagtc gagatttatg
    13621 ctgacgggat tggcggtgga gatgctcttt gtttgccgat gagacgtggc aggagacttg
    13681 ttgggacgga gaacggttac atctatacaa gagaggtgga gactgaccgt ccggtatcgg
    13741 acttcacagc aagattgatt ccgtcctttc caggggtggc agttcctctc gaagcaggtc
    13801 atatcttatg gcagaaatga ggaacctcct ctgtacaatt caaagtacga atggcgatgt
    13861 gatataaaaa ttaacccaga atgtaagtta ccggctgaaa tgaagagatt ttcgatagag
    13921 gaagtagtaa agcagggttg ttgaaagtat caccatggcc attacaaaat ataggccacg
    13981 atatccggta acaggaacta aggatccgaa taagtatggt cctaccccga accctatatc
    14041 taggaacata taataagtag cagttgccag tccaaaccgg ttgggtgagg tgctttttac
    14101 ggaaattgcc tggccacagg agaggaagtt cccatagccc aagccgatga ttgctccgga
    14161 taataataat gttatcccat aggtggcctg actcagaaca agcataccga tagaaaaaaa
    14221 gaggatacag ggatatacaa cgaaatttgc tcctctggca tcaagtagac gcccggaaaa
    14281 aggccttgag aatataacaa caattgcata tatgagaaag aaaaagcttg ctgcttcttc
    14341 taaacagagt tgcctgctgt ataacgataa aaaactcaat acaactgaat agccaaagcc
    14401 aacgataagt acgataatcg aaatcggtat agccctgaat tcaagaaact ctgaaatttg
    14461 aaactgcttc acggttttag tctggacctg tttgggtggt ttataggagg gctgactgac
    14521 aacaaaggat attgcaaaac tgatggcagc caccagtgac gcaatgagaa aaatcatctt
    14581 aaaatccaca cgctggctta agacgatccc taaatatggg cctaatgctg ttgccagtat
    14641 ttgactcatg ctgtaatacc caattccttc gccgtgtctc cccctgggaa ttatctgggc
    14701 agctatggtt ccggtagctg tgtttgctac cccaaaagca aacccgtgga gaaatctcat
    14761 cagtatcagt aatggtagat ttattgctgc gaaatataat gttgatgtga tgatatacag
    14821 tagtgtacca atgacaagaa ccctcctgct ccccacatcc tcaataatac gtccggtccc
    14881 taatctaccg attaagatcc ctataataaa aatgcctgaa acaagcccgc cggtacctgt
    14941 agtagcatgg tatttgtcta cagcatatga ggccataatg accatgagca agaaaaatac
    15001 aagataaatc aggaaattgg ttgtagacac tacgatgaaa tcttttgtcc agagcctttc
    15061 cttattcatg tattggcctc taagaatatg gatagaatag cttccacctg gattctttta
    15121 ccgttagctg ctttcacaaa aaccgagcgg acacatgctg ttcgtctgcc taactggcaa
    15181 tggctatttt aaaaatgagg gtaaatgcta ttatatctaa atactatatg acgaatcttg
    15241 ttatcaactt aaaaatggaa tttgcactgc tacctccttt gagcccacac acagctcaac
    15301 acttatggtt tttcatggtg ctgcttgttc atgggcaggc tttagtaacg tgagtcatgt
    15361 ttatctccat agatgtttat gggaaaggat ttaagggttg atgtttgccg tcccaccaac
    15421 agtgattgct gccagttgga atattgagtg aatcaagaga aaactattgt caaatcaggg
    15481 cgtaagaatt aaagccattt ttttctccgg aaatagaaga tcatcagcac ggctataatg
    15541 atcacggcaa tccagaatga gacgtatgcc cagcgccatt caagctcggg aaagtatttg
    15601 aagttcatac cgtacacgcc tgcaagaaat gtcatgggca taaatattgt agcgataact
    15661 gtgagcactt tcataatctc attcattctg ttacttgtgc tggacaggta gatgtcgaga
    15721 agcgaagaga ttatatcccg gttagtctcc atggtctcga tgatctggac cgtatgatcg
    15781 tagatgtccc tgaagtaggt cacgctgtct cttttgacca ttggaaattc atcatgtgtc
    15841 agggcactca cgacctccct gagaggccag accgatcgaa ggatcgtggt tgtttccatt
    15901 ctcaacaaat gaatagcctt gagaacatct ggtgtaggat tgccaacaac cctatcttcg
    15961 aggagttcaa ggttgtcgcc aattttttca accatgtcaa aataactgtc gaccactgca
    16021 tcgagtagcc ggtaggtgag gtaatcagtg cccatcctcc gtatgcgccc ccggtatttt
    16081 ctgagctttt cccttatggg ttcaaagacc acgccacctt cctcctcgaa ggagagcacg
    16141 gcctgtctgg tgatgaccaa gctaacctga gccgtcatca tcctatgact cttctcatgg
    16201 aaatgaaacg tcttgactgc tgcatagata taatcgacca tatcctcgag ctttggacgt
    16261 tgaccggtat tgagaatgtc ttctattgtc agggggtgta tcccaaagat ctcgccaatc
    16321 ctggccaggt tgtccgaatc agggagacca cgaaaattga tccaggtgac agtaggtgtc
    16381 agacggtagg ctgccagatc ctcgatccgc tctatatccc tctcatcaaa atatttttca
    16441 tcataatcaa taaccgtgat tccagacagt tctgcagatg ttccagaatc agaatcctct
    16501 tttttcatca tggcaacctc gcgttttact tgagataaat attaccttca agcgagcatc
    16561 ccagtgacta cacgaacagc acagggccca gggtctggag atgtcaacca tcctatggca
    16621 actatagtta cgaacatatc tctgaataac agggattgaa aagcctgtca acacatattt
    16681 tgtaccaaac tgatgccgat gcaggccttg gacgtgaatg aggcacttgg gtcttcacac
    16741 cagggataaa tggagaaaat gaagtattct gggagataaa gccacgaaga gtatgttatt
    16801 gtgacatggt ctacttttgg ctttaatacc catacgatat gaaaacagag cttgatgacc
    16861 tggccatcaa cgtattaact cttagcatct gcgtcctgta ttgaaactgt gagcatgtct
    16921 gtccccgatt ttgcccacct atgctacccc cgcttctgat agccacaatg agcaggctta
    16981 atattaattt ccaggatctg gatccttaac gccaatgcat acgcccttac tgaaggaaca
    17041 tcaaatttat ctgtttgggg ttgtatctct tcatgtccaa tcggtgaatc aagaacagcc
    17101 tgattatcgg gttccagaaa gtataggggt gaataaaata aaaccgggga aacatctttc
    17161 tccccggttt tattttattc tgtaacgttg ggttacttag caggggcggg tgctgtagag
    17221 gggcctggtt ttgtttcttc tttcggtgct ggtgctttct tgccagcctt ctgaatagtc
    17281 acagacttgg caatagtctt gccgtctttc tctgcgtatt tcaccaagac cttgtcgcca
    17341 gctttaggct ccttcttcat cttgacatta gctacatcga aagtcttctc atctgttttg
    17401 cctttgatgg ttagagtctt tgcagcggca tcaatggcaa caacttcacc tttgtactca
    17461 tgggccttcg cttttgccgg cgctttcttg accaccttct ttgaaggttg agacgtacct
    17521 gcagtgtctg ttggtccctg tgcaaaaacc gctgttatca gggaaacacc cataaggacc
    17581 gccaatgtga caatcaaaaa tcttttcata ctgtacctcc ttttgtggta gaataatatt
    17641 atactgtaat ttagcatcac ctgtgccaca gatgtaaccc tctatagata aagaaaaata
    17701 gaaaacactg atagtgctgt gtactgtcca ttagtcggat aaatccgacg gaaaagcctg
    17761 tgacatgacg gataattccg atcaccgggg acaagaattt ctttcttgat atatttctaa
    17821 atatgagcca tggccaatgc aagaacgtca atgaacaact gtttcatttc acaccctatg
    17881 tgtttcttcc ttgctcacac cctgtccact cgaaacccgg tacccatcat ccttagaagg
    17941 aggatcatag accatttttg ttgatatcat gggcgtttct cctccgggtt cgggtctgtt
    18001 gaccagatat cggtcttgtc gtagcaaatc tcatgaataa gatccctgcc atatttccag
    18061 ccctcatttg gcagggttct ttttcaacag gcagtatcag taatctcctg ctgattattc
    18121 aacaatcgct tcataaaatg tcggcgtcgg atttccgtta aacaggggtt ggacgcgttt
    18181 accagcttca tcatgagcag gactggtgtg catggccatg aatgtttcct ggctcttgtg
    18241 ctccacaata gctgcgtaat taccaccctt caacggcttg agaagtcgtc tgctgataaa
    18301 accctcatgt tttgcaaatt ccgtattgga ccaactgaac cattccctga actcagcatc
    18361 tttgccatcc ttgattggtg gaaaattaat aataacgata aacattgtga aacctcctta
    18421 gatattggta aacgccattt ctaaatagac gtaagttatt ttacctccag tgaatatggg
    18481 tatctggtaa gcacaatcgg ctcctgttga ccaacgacca cagtatcctc caccctgact
    18541 ccccaactgt cggtgtaaag cccgcaattg ccaatggcaa ttactacatt tacaggaagg
    18601 ggtggcggtt ctttttcgcc atggaaaaat gcatgtcctg gaggcaaggg ggattcctca
    18661 aagtcaatcc caaccccgtg gatgggcggg ccaaaaatat gttccccata tccgccatcc
    18721 cgtataattc catgcattgc ctcccccaat tcaaccatgc caatacctgc ttttgctttt
    18781 gcaatagatg cctgttgagc cttgagatag aggttgtatg ccacctgctt tccatttgac
    18841 ggttttccca cgcatacggt tcgacaaata tccgagctat acccattgac tatagggtgt
    18901 atatcgatca ttacaaggtc accagtttca agcttccggt ttgttggcag cccatgggca
    18961 ataccggtac gggggccgga tgataaatag gtgcgccaga aatcttccgc gccagcatac
    19021 cgcatagcgt attcagcttc tgcagctatc tggctctctg taatcccctg ttttactgct
    19081 tctattgctg ccttcattcc aatgtcagca accactgctg cctgcctgag gagatctatt
    19141 tctctttcct cttttacaag tctaaggctc gacagaatag gggtgcaatc ctttggcatc
    19201 acatccttag gtttggcgtg aggatgggtt acgatgccca tcatggactg ggtgaggaag
    19261 gtaaattcca atccaactgt cccttccttg aggttgaagt gttcaaagta gtgtgcaatt
    19321 gaatgaatca tgcccacctc atcctcgaat ccgacaatgt gatggaaaaa ggcgccctgt
    19381 tttacatcct caatatctgt cttgggtacc cccatagcta cctttgcatc tgtggtgatc
    19441 atggcatagg cacagaggcg gccgtcacct gtgagatact gcatgtttga tggttttgta
    19501 agcaggagaa gatcacatcc ctctgttttc atcatttctg tacatcgttt gatccgagcc
    19561 tgatatgtat ctttatccat cgatgaacct ccgcacataa aagtatttct tttcttgttg
    19621 cgttatctaa tcatcttaat tcctgtgtgt attctgaagt ccaacgtaat gcctctatac
    19681 cgaatcttga tagctttgca gagtctcttc catgtaatgg gacaaagaca tatacgcttc
    19741 tgtgtaatct tacaacattg tccagtctgg agttttttaa gagatagtgc atcttgcaga
    19801 aacttagcat ccctgccatg cttccccttt cagtttttca ggataaatat ctcctctttc
    19861 caggttgatt cattatctta tgtcatcaat tttagacaga ggaagaggat tgtcaatgaa
    19921 agttgagtat gtttctgaca atccggggaa agggaatcaa gatgataatg accttgtcta
    19981 agcctccata atcaatggaa ggaaacccct gattatcgcc gacctttcat gttttttccc
    20041 agcacgacag aggtttcgat ctgacgttcc ggaatgtccc agtatttgcg cagcaattca
    20101 tctttgttac ccatcagttc gtacccgtgc ttcttataaa aatctatagc ccaggtagcg
    20161 gctttccagg tgcctacgat gagccactct gtgtcaacct gtgtctctat gaaatgcagg
    20221 agctttgacc cgatgccttt gccctgttgc ccggttagta cataagcgtg acggatgaga
    20281 gttgtgtcct tgatgggttc ctttgccatg acacccagga gatcaagatc tttccgatat
    20341 ccgtaaaaac gcatcctctc catctctgta agtaactcat tcattggcat gtatggctcg
    20401 tggtagcaat cattggggat gataccactg tagcgctttg atccattatc tatgaccgta
    20461 aaaatttcat ttgggttttt aacctcaaaa ataccttcct tccagaatcc gaagaggtta
    20521 taagcgttct ggaagcctat caactccatg gctgtatcat ctttgataag ctctctcgct
    20581 tcttcatagt ttttcttgat acccggtacc cctggaaaat cggttccgaa aagaaatttg
    20641 tgaaccaact tcttcatgga gggccagtag gtcaacaggt ttttcggagg cagtcctgat
    20701 atatcgatat agacattttt gaagctcctg acaagaagtt ctgcaatctg gtaccagaac
    20761 ccccttccgc cgtgacacag gataaccttc atatccggaa aatcattgat cacgtcatca
    20821 aaggtgtaag ggtctgaata tcgcatcttt gaaccactga ggagactgtt ccccgtgtga
    20881 aacataacag gcagtccttc tcgttcgcac ttctcgtaaa caggataaag ggcctgatca
    20941 ttggcaaaga aatgcccgtg gattgggtgt agcttcaatc ctcgtgcccc cattttcaac
    21001 tgcttttcaa atgcccccat aaggaccgcg gaatggctga actctcgttg ggccaacgct
    21061 ggaacttccg gttgcattgc tgcggggtcc cgatggtaat tgggattgag ggaggctatt
    21121 ggtatgagtt caggatgata agtatggacc tcggctgccc cttcaaatgg gaggacacca
    21181 ttggtttctg gagagtactc cgggatcagg ataccaccaa ataccccttc atcctttata
    21241 acatcaccgt actgttgcga aagcctgttt ccggacttgt caaatatttt ggtaacgtac
    21301 ggacctgtat ccatccagac ggtgcgtgcg gattctgtcc attcgtacca gtgccctaca
    21361 tggatgtgga catctatgat cctcatgtgt tgtttataac gtatgaaagg aatgttggca
    21421 agtatttgca gaggaaaaaa gactcgtaaa gaaaaatgat tactacccat gaccacatca
    21481 gaaatgaaaa tggattggga atatggtaat gtaacaacca tgccgggaaa tatgtttttc
    21541 atgcttggcg ttattcatcg tgataaatat ggccccgttc tcctgaaata ctggttggac
    21601 cgtatccaac ccgattctat aaccctggag ttgtcccatt acgggatgcg attccggagg
    21661 aactttagcg aagagtatac gaggaggatt gataagataa ttgtcaaccg caaagaaaaa
    21721 ggcgaaccat gtaacgaaga agtcatggca tcactcctgt cttatgtgag aatgccctac
    21781 gagtacgacg tagtctctga ttatggaacc gaacatagca tcccgttcta tttaatagat
    21841 atggactttt tttcttatgt gaaactgaga aacatagaag atttgctcag cgaggagaac
    21901 atcgacagaa tgttctcgtt tccagaatta cagaatggta acctggaaat gacaacagca
    21961 cgactctact tcgaaaaagg tattgcactg acgaggtacg gcaaagagat gtatattcgt
    22021 gacagataca tgtccacaaa gattcaggac ctgatgaaac acagtaataa gaaaaggctt
    22081 gtccacgtgt gcggctggca acatttgcag gatccatcag ggctctacaa cgcactgaat
    22141 cccattaagg tttttttcca tgataaaact gtttgtattt gatctgggca acgttattct
    22201 ccctttcgaa caccggcaaa ttgcagtgaa gcttcacgaa aaatcaacag aaaaagaggc
    22261 attcacacca gaacaacttt ttgatgtaat gtttgacctc cagaatggtc ttataaaccc
    22321 ctatgaagag gggctgatgt cttctgcaga gtttctttct aaccttaaac gacagtttcg
    22381 gctggatata gatatgaatg ctttcagcaa tatctggaac cctatcttcc gtgatgatcc
    22441 tgaagtcaat aaagtgattt tctacctgaa ggaaaaaaga tatcctctgt ttctgctgag
    22501 taataccaat gaactccact tttcctacat aatggagaac tatcctattg ttcattggtt
    22561 tgacgaatgg cttctttcct ttgaagtggg tgcaaagaaa ccaaaacagc gtatttacga
    22621 tgctatcttt gaaaaaatgg atgttaaacc gaacgaagta ttttatgttg acgatatctc
    22681 aaagtatgtg gaaacagcac agcaacttgg gctacatggc gtggtcttca ggagtgctgc
    22741 tcaactgtgg gatttcatcc agaaaaatgt tatataagtc agctgacagc tcatggctct
    22801 cagcttaagt agttattttg ctagaagctt acagcttaaa gcttgcttcg aggtgttgtt
    22861 gtgctcccgt atcccaacat tgatcctgta atattcagaa taggtccatt tgcaatgcgc
    22921 tggtacgggc ttatgtatgt ctttggtttt gtatcttcct atctactctc agcctatcag
    22981 cttaagaaga aagcgtttac tttaacaagg gcgcaactgg atgatctcta tttctatctg
    23041 atccttggct taatcatagg tgcccgcctc gggtatgtga tcttctataa ccttaatttc
    23101 taccttgaaa accctttgga agtttttgta ttgtggcatg ggggcatgtc cttccatggt
    23161 ggcctgatag gatcatttct tgccggctat atctttatta aacgaaaaaa gctcaggttt
    23221 ttttctgttg ctgatcttat cattccaact tgtccccctg ggctcatgtt tggcaggatt
    23281 gggaacttta ttaatggcga gctttttgga aaaccctctg atgttccgtg gtccatgatc
    23341 tttccacagg gaggaaatat tccccgacac ccatcccaac tctacgaggc ttttctggag
    23401 ggttttcttc tgttcattat tttatggata tataagaacc ataagaagcg ggaaggagat
    23461 gtttttgcag cgtttcttgt actctacggc atcttccgaa taatctgcga gatttttagg
    23521 gtgccagacc tgccagttgg ctatattctt ggtttcctca gcatgggcca ggcactgagc
    23581 attgttatga tcattgtcgg gctcctgatc aaatttgtct atctaccacg agcaggccag
    23641 catattgaga ggctgagaaa gtaagaagta gggcagcagg ggctgtctat tctattattt
    23701 tgcacctata gcctgggtac agtcaactaa ccaaccactc aaccttctaa cagttccact
    23761 tcccctgttt caatattata gtatgcgcct atgacttgaa catcgccctt ttctatcatt
    23821 tcgaccataa taggctttga agacttaatt ttgtttacca ccaatgatac attagcccga
    23881 atagcattgt cgacaagatc tcctttcagc ttgcgggctt tttttatggc aggttgaatg
    23941 gccttcacaa tactcccaac atgaccgggt gcgtctgcac ttttagctgc tgcggtgaca
    24001 gccccgcaat tcccatggcc gagcaccact acgagcttgg tggcaaggtg ttcaacagca
    24061 tactcgatgc ttcccatagc aaggctatcc acgatattgc ctgcaaggcg aacaacaaaa
    24121 aggtcaccaa gtccctggtc aaaaatgagc tccggtggga tacgggagtc cgaacaacct
    24181 actataacgg caaaaggttt ctgacccttc gttacttcct ttctacgttt cgcatcctgc
    24241 cttgggttct gttgtgtgcc agcagcacaa cgcttgttac catcaagtaa tttcttcaga
    24301 ctttgtttaa cgtttaaagt ctcagccatt ttacctctcc agtataagtt tttattaaga
    24361 taagctatat tacgagattt tgctgccgcc ttcaagatat ttttcacaag gatgtttttt
    24421 cgtttttgta tacaacctat ttttatattg acaacatctt cataccacac tataattgtg
    24481 aaaatattaa caatatatta ttgacatcac atacccttgt gtttaaaata gattgtttaa
    24541 aacaagaaag ttataagcag taatatccca acattaaatc aggaggttta gcatggcaga
    24601 agttaaggaa gaaaccaaat atttttatcc cccaaaggaa ttcgtggaag aggcctatgt
    24661 gagcagccgc gaagagtatg aaacaatgtg gaaacagtca gtttatgaac cggaagagtt
    24721 ctggggaaac attgcatcag aactgacctg gttcaagaaa tggctcaagg taaacaggga
    24781 agacttctca aagggccaga tagagtggtt tatcggcggc aagaccaata tttcctataa
    24841 ctgcctcgac gcacagataa agaaaggcag gggagacaag gtagctattc tgttccaggg
    24901 tgagcctgaa gatgatgttg tgaaacttac ttacaaagat atgcttacat acgtatccaa
    24961 gtttgccaat gttctgaaaa agagaggagt gagaaagggc gacagggttg ccatctatct
    25021 gcctatgata tggcagcttc ccgttgcaat gctcgcatgt gcaagaatcg gtgccatcca
    25081 cacgatcgtt ttcggtggat tctctgctga ggcactccga gacagaatcc tcgatgccgg
    25141 cgcaaaggtc cttattacag cgaatggcta ctggcgttca ggcaagaatg tgagctcaaa
    25201 ggcaaatgca gatatcgcat gcgatctttg tgcaaaagaa ggccacactg tggataaagt
    25261 catcgtagtc aagaggcttg ataagtttga tgttcccatg aaggccggaa gggacacctg
    25321 gtttgaagaa gagttagctg cttcggatat cagcgatgtt tgtcctgcag agtggatgga
    25381 tgctgaagat cctctgttca ttctttatac ctcaggttcc actggcaagc ccaagggcgt
    25441 tcttcacaca gtcggtggat atatggtgta tgtgtacata tcattcaaat atatcttcga
    25501 ctataaggaa aaagacgttt tcttctgcac agctgatatc ggctgggtaa ccggtcactc
    25561 ttacatcgta tacggaccaa tgtgtaacgg tgcaacctcg attgttttcg agtctgttcc
    25621 tacttttccg aaccctgaca ggttctgggc aattgttgag aaattcaaag ttaattcctt
    25681 ctatacagca cctacagcta tccgtgcgct catgaaagaa ggggaaaaat ggccacaggg
    25741 cagagatctt tcctcactgc gagtcctcgg gtccgtgggt gagcctatca accctgaagc
    25801 atggctgtgg taccacaagt atattggtgg agagagatgc cccatcaccg atacgtggtg
    25861 gcagactgag acaggtggta tccttatttg tgcacttccc ggagcatggc cgacaatacc
    25921 cggttttgca acgctgccgt tctttggtgt tgacgcaaga gtacttcagg cgagatcatc
    25981 tgcaaaagaa ccagcagtgg actcaccggt caacgagcag ggcgagctct gcatcggacg
    26041 gtcatggcct ggtatgatga gaggtgtttt tggcgatcct gaaagattct ataccaccta
    26101 ctttgtccag cagcctggtt tctacttctc cggtgacggc gcaatcagga acgaagacgg
    26161 ctacttcagg ctcatgggcc gtattgacga cgttgttaac gtatccggtc acaggatggg
    26221 aactgcagaa gttgaagcag cccttaactc cctcactaca gctgttgcag agtcggcagt
    26281 tgttggcttc ccgcacgaag tgaagggcga agacctctat gcgtatatta ttctgaaatc
    26341 aggcgttgag ccaagtgatg ctctgaagaa agaactggta gcccacgtaa gaaaggagat
    26401 cggtcctatt gcatctcctg gcaaaatcca gtttgtttct ggtctgccaa aaacacgttc
    26461 cggcaagatc atgagaagga tactgagaaa ggttgccgca ggcgcgatag acgagcttgg
    26521 cgataccacg acacttgccg atccagcaat tgttgatgag atcgtgaagg gcaggcagta
    26581 ggatttatac tacgtaaaac tagggccccc gggaaccccc gggggtcttt ttttatcgca
    26641 aatagatgat ggacccagat aatgatcaaa agaatataaa caagaacaac tcaaaaagtt
    26701 gatatcaccc tagattttct attagaatgt tcaccggtgt cagctatctc ttacaaacac
    26761 gttcattcgc tcctgttcgg ttgcagaact tttgctggcg caaaagaccg tgtttcccca
    26821 tctatcgacg gtttgctcag gatgaccaga tgttatgctt catggagctg cctattgatg
    26881 agttaacgct atgtttagta gcgctatgca tttgcaggaa gaataggcta tggctaaaga
    26941 ctacacactc agctcaacga aatcctacca cattgggggt ctgaaaagtc tcgggtggga
    27001 acttaccatt tgcaatgccc tccaacagga gggaacaccg ctgaggaagt tgcttacctg
    27061 caatgaatct tttggaagac ttctctatga ttgtctatgc acattcctac ccctgtggaa
    27121 tgtgacacac gttctcgaag tcggtggagg ttacggctac ctcatgaaag actttctgga
    27181 aaaagatagt tcgctcaagg cgtgtatgct tgacatctca ccgtttcttc ttaataaaca
    27241 gcgggaaacc ctcaaggaat tttcagttga atatcgagaa ggtgatttct tggaagtgtt
    27301 gccaagcgat ctttcagcat tcgaccttgt catcatgaat gagaaccttg gagacttccc
    27361 atccctcata aatattagaa ctgaagtttt tgacagcaag attgccaaca atgatcctgt
    27421 tctaaaatgg gtgcagggga tttttgaaaa gtatggtctg gaacggccag agggaaaagt
    27481 ttttaattta aatatgggtg ccattgaggc gcttgaaaaa ctttgctgtg caaaagttcc
    27541 ctacatatat ataggagagc acagttgtga ggcttctgtt tcaggggaaa tgagctcgct
    27601 ttttcaaatc aaatcaaccg ggaacccgga acggattacc cttgctggtc acgacgagta
    27661 cacaataaaa ttctcctacc tccaaaaaat agccagtttt catggttacg agtctatccg
    27721 gggccctttt gccgatttta taccccttac cctgacagac gaggctcgct tcgctctgat
    27781 gtacggtggc cactattctg atgaagcaga ggtgatgagc cagtttgttg aagatctcta
    27841 taaatatgaa tatctcatac taaaggaaaa ggaggagcca atatgagtgg gtaccatgaa
    27901 cgaaactggg aaccctttcc catggattca ataaagcggg ttgatcgacc aacaaccgtt
    27961 atacacgaag atcaggttaa aagggtagat gaaagggaga gcggattcaa cagggcgttc
    28021 aggggtgatt tcggcgaacg tctctctctg gaagtccgcc ggtttgttcc caagcaaccg
    28081 gtctcaggtg ctctgctgtg gatgaccagc aatatgatga ggatggtcga tggccttgtg
    28141 gcagccaggc atgcaccgct accggaagac ccggctgcca tatcccgaca tatcaaggag
    28201 cttgcctatt ttctcagggc tgatgcagtt ggtatctgca aacttcccca gtatgcagtc
    28261 tatacccaca agttcccaaa gggagatcca atcgaattga atcataccta tgccatcgct
    28321 gtgctcgttg atcaggactg gagaacagcg aacgcattta ccggaagtga ttggatcagt
    28381 aatgccatga gcttcctggc ttattcaacc acagggttta tcgcatgcat actggcagag
    28441 tatattcgtc gtctggggta ccctgcccgt gcacaccatg cacgaaacta tcaggttgtt
    28501 ctccccccca tccttctgtg ggcagggctt ggtgagatgg caaggatggg agactgcgtt
    28561 attcacccct tcatgggacc gcgattgaaa ggggctgtgg tgaccaccga tcttcccctc
    28621 ctggttgata agccgataga ttttggcctt caggatttct gttccaaatg taaaaaatgc
    28681 gcccgcgaat gtccgtccgg cgccataagt tctggtgaca aggttatgga caagggttac
    28741 gagcactggc cgctcaacgc tgagcgatgt acaagaatgc gtgttggaaa caagaggggg
    28801 tcaggctgtg gtacatgtct caaggtgtgt ccgtggaaca agccatacac gctctttcac
    28861 cggggagtga actggacaat gcgccattct gcccttgctc ggagccttgc aatcaaattc
    28921 gatgatctcc tcggatacgg gaagccccac tacgaagaca aatggtggtt tgacctggag
    28981 gaggtggatg ggatcatcga tgttccccgt tacagtgtca aggatcaaaa ggatttcaag
    29041 caggacatag attattagca aaactgtatt ttgcatctgg gtgaatatgg tatcaggcat
    29101 tccgcacaaa tgcgtccatt tgcaccagcg gccttgctcg ctcgcagcca acgggaaggg
    29161 acgtctgaat gggggaccgt acgtcgcagt ctccttacac ggtgaagtca ttaacatgat
    29221 tggaattgac atggaaaggc atacaggcta ctatgtaaaa cgtttatgaa gggcaaaaag
    29281 gtagcatttt ttgatattga caaaaccatc tatgatggct atttgatctt tccactggcc
    29341 gagtacttcc ttaaagagaa cgttgttggt aaggatgttg tccatagtct gtataatgac
    29401 ctgcacctgt atcgatcaca acaggttgat tacgagacaa ccgtcgagaa cttcaatatt
    29461 cacttggcta atggtttaaa acaccgcgac actgattcaa tccttgcctt aacaaagagc
    29521 tttctcgaga ctgacgaagg caataagttt tttccctttg cagccgatct tatggaattg
    29581 ctggaaccaa catatgatat ctatttcgtt accggcgagg ttcaatgcgt cgggaaagca
    29641 gttgcagagt actttgctgt gcatggatat gtttccacgg agatggacat taaagatagt
    29701 gtgtacaccg gtaccattcg caaatccctt gcaaggaagg aagggaaaag agatgctatc
    29761 aaccacgtca ttcacaagta cccccatgaa ggttcccttg ctttcggcga ctcagaaggc
    29821 gatattgaga tgctcgatgc agtagcgcag gccttttgca taaatgcaac agaaggtctc
    29881 agggaagttg cactttccaa gggatggcat atcgccaagc cagaaactat tcttgatgca
    29941 gtgaagaaaa cactgtaata tttttatgac aaaatctgtc agaatgggtt agcaacttta
    30001 ccctcacata ttgcaatcca aacacccaca cagaaggcga tttaaggttg tgcccttgta
    30061 cacggtgagg ccaaacccat cactgctgcc agtccattgg gtaagaatcc caagaatgca
    30121 aagtctgaca aattgatagg tttcttcagc aaaactattg cttcagatac ggaactgagg
    30181 ggtagcgact gcgggcaaaa catagaaaat agtcctgcac gggaacaatc cctcatacat
    30241 cgctttttct gactcctgat gtcctccatg caggtgagat tacactgtgg ccctttttct
    30301 ggttgaccgt tgaagcccag gtgatcttgt tctcgccgaa cttgtcattg actctgtcca
    30361 tagccttcaa cagggcatct ctgtctttac ggtgtggttc ctcaaagagt gttaattgtt
    30421 cattctttcc gaaggatgag agagataccc caaggagccg tatgcttgtg gtcagatgta
    30481 ttccgtcaaa gagggaaagt gctgtccggt aaatctcacc ggtatcgttg gtacagtcgg
    30541 gtatggttgt ttgtctggtg aatgtcttga agtcagcata cctgactgtg agggtaactt
    30601 tttttccttt gtatccgtag cgtcttgccc tccttcccac catctcgctt aggctgagaa
    30661 gctgaggctc tatgtcttct cttttccaca ggtccttttc aaaggtcctg ctgtggccta
    30721 ttgatttcgg gtcactgtca gcaggttcca gaggtctatc caggataccg ctgcccattg
    30781 ctttgagctg gctaccaaag acaccgaatt tttttataag aagggaaagg ggtgccgtac
    30841 ccagctcacc gcaggttttg atccccatgg cacggagttt ctcttctgtg tgggggccaa
    30901 tgccccacag ctttttcaca ggaagcgttt cgaggacaga ggcaaccatc tcctttttga
    30961 tccatttgag gccattgggc tttgccaggt cacttgccag cttggctatg gggatgttag
    31021 ggcctatgcc tatggtacag ttgatgccga gctcttcctt cactgtctcc ttgatagctc
    31081 gggccatctc ctcagggcct ccaaaaaggt gatgcgatcc tgtgatatcg aggaatatct
    31141 catcaatgga gtaggtttct ctgtcagggg tgaagcggtc gcagattgtt tcgagttttt
    31201 cacaggtctc ggcatacctc ctattgcgcc cggtgacaaa gatgatatgt gggcagagct
    31261 tctttgcctc acggggagtc attcctgttt tgacaccata ttgtctcgcc tcgtaagaag
    31321 aggtggtgat gaccgttctt tcacctacac cggtaactgc tatgggcttt cctttaagat
    31381 cagggttgtc atgctgctca acagaggcga aaaaggcatc catgtcaacg caaaggaata
    31441 tcctggacat gattgagtat atacttctta tgacgcatag gcaagaattt gagtatattt
    31501 gaggtaatgg ctggatcagg aatgcactgt tttttcatcc atcaatactc accggtttat
    31561 cgaggaaaac ctgaagctac tacacaaccc gacattaata agacaacgaa caaagctaca
    31621 aggcagtctc tatctttgca tccacaatga tgcatccttt accctcctca taggcaatga
    31681 gggggttgat atcaatgttt acgatttcag ggtggtctat taaaagtttc gatatcctgg
    31741 aaatgcagtt ggtaatggcc ttgaggtcat atggtggatt gccacgatac cctctgagaa
    31801 ttgcagatcc ctgcatatcg gttatcatcc tgagggcagt ttttttattc accggcacca
    31861 tgcgaacagc aatgtctttg aacagatcta catagatgcc acccatgccg aagataagaa
    31921 catggccgaa ggcaggatcc agctttgatc caatgatcag ttcaagcccc gtctgaacca
    31981 ttttctgtac caggtatgga ccaggctcca tgctattgaa tgcatcctta agggatgaat
    32041 cggaggccac atgaagcctg accccgccct tttcggtttt atgcaaaatg cctgacagtg
    32101 cggtttttag cgctacaggg tatccaagcc tttgtgcggc attcctgcac tcttcaaaat
    32161 tccttgtcac ctcgtaatcg gccacaggta gatcatactg gcggatcaat tcaaaagcct
    32221 ttgcaggaga caggaatgag gtctttgtat gaggcgaaac gtttgtcctc acgaggcact
    32281 tccttcgttt tacagcagaa gccctcagag tggctgctgc tcgcctgaaa tggcttagag
    32341 acctggcaag cgccttcact gcctggtctg cctctgaaaa aacaggaaag cttgccccat
    32401 ctttcatatt gaaccattgc tccttttcag agagcatgca gaataccact ggttttggtt
    32461 gtacccttgt aatggcattg attttttgaa ttaaggccag tgttgcgtcg ccatccgttt
    32521 gaaggtcgta agagtgaatg aagagtaccc cgtccacacc gttctctttc agggtttttt
    32581 ccagtatggt gtaatggaag ttaatatcga agatgtcacc aagatcaaca gggttggttg
    32641 ctctgattac atcaaccctc tttttttgtc ccacgaggtc gaaaatgtct tgggaaaggt
    32701 gtgccaacct gaaaccgtgg cggtatacag catcagctgc catgaccgcg tgtcctccgg
    32761 accgtgctat cactgcaagc ctttgacctc tcatgagtgg cagggaaaag attttgatgg
    32821 tactgatcat ttcatcgagt gtgcggaccc tgtggattcc tgcctggcaa aggatgttgt
    32881 ctgcaataag atcatcacca gcaagggagg cggtgtgaaa ttgcgcaatt tgagaactac
    32941 cggaactttc attggccttg aggaggatga ctggtttatt ggtagaagtt gcaaggcgaa
    33001 aaaggcgcct tccgtcttta atgtcttcca ggtagaggcc gatggttttt gttgcgggat
    33061 cagaagcaaa gtactcgagg aaatcgcact cgtccagcat cagcttgttg ccagcactaa
    33121 cgagtttgtt gattccaagg ttgtcacagg acaaaaggag gagtatgtcg tgcaagagac
    33181 ctccgctctg ggagataagc gagatatccc ccttctgtaa aaaagtctcg tgaaaacgaa
    33241 caaatggtaa aaccaggcca ttttccagat tcacggtgcc gaggcagttt ggtcctagga
    33301 ttctaatgcc ccattgtttt gctatggcga gaacctcctt ctccaatgct tctttttcct
    33361 ttccaaactc agagaaaccc cctgactcaa tgattacatg ccggatgcct tttttgccgc
    33421 attcttcaag tttgccaggg atgcctttgg cgggtattat aaaaatagca agttctggaa
    33481 tgccgggaac ctctgcaatg cctttcaaga tagtttttcc cctcaccgac ccgcctttct
    33541 ctccaatcag ataaacatcc cctttgaaag aaaaacgctc cagattttcc aatattccaa
    33601 tccctaaatt accaggcgaa gaagatatgc caaatatggc aatactgttt ggaaagaaaa
    33661 actcgttcat aaatgcttcc ccttcaattt taaatgttct atgcaactta tgcaggtaag
    33721 gcgattgtat cacaaaaatt ttggatagat tataagaaat ttgcttagga atcttttgtg
    33781 caataaaaaa aggcaaaccc cattgaagtc tgcctctttt taccttcttt atcgattgtg
    33841 gtttctattt ttttggcggt ttcaggagaa taagagcaat aatgatccca aggattgcca
    33901 tgcccgctgt aggatagaac gctattgtgt agcttccaaa ggcgtccttg gcgcttccag
    33961 aaatgatgcc cccgatgatt gcaccgagac cgtatgagct aaacacaaca ccatatttct
    34021 gtgcatatcc ctcaagacca aagaaacctg ctgttgctgc cgggcctatt gcaagccagc
    34081 ctccgagaca gagccagaat atgcaaaagg cgactgcata cagaccaacg gatccctgac
    34141 cggctgcaag catcaaggct gaggcgacaa aaatgagaac gaatgacagg attgcagacc
    34201 acttgggggt tattttatct gtaagggcac caaagattgg gcggccgata ccgttgaaaa
    34261 cagcaaatat cataaccagt gttgcggctg cacctttttc aaggcctata acctctcttc
    34321 ccactgcagc agatatgccg attgccatga gaccggctgt ggcaccgaga atataacaaa
    34381 gccaaagtcc gtaaaaactt cctgttttca tcatttgccc tgctgtaaac gatgatgctg
    34441 ctccgccggt ggctgttgtt ggagggttcc atccggctgg tttatatcct gcagggggga
    34501 atttcatggg cattgaaagg ataatagtta taaagaaaaa cgcgataccc aatactccaa
    34561 acgctccgag aggcccacat ttgcctatga gggcagaagc tattggggca gttgctatgg
    34621 ctgacccgcc aaaaccggcc aatgctaggc caacagcgag acctttttta tcgggaaacc
    34681 agcgggttgc aactgagaca ggcccgccat agacgatgcc aacaccagca ccgccgatta
    34741 ttccataggt gagtactacc atccagatgc tggaagcgaa gtatgtgagc atccatccga
    34801 taccgacaat ggcgccgccc accatcatga ctttcctggg gccgagtttg tcgatgaatt
    34861 tacctgccac gaaggtgaaa agggcgaaca tggcaagaaa gatcatgtat ggtaaaccgc
    34921 cctgcgtggc acttatgttg aatagttctt ccagtggttt tttgaataca ctgtaagcgt
    34981 aaacagcacc taggcagaaa ttgacaacca tgcccagaat gacaaagatc catcttcctg
    35041 cacttgcaga cattccaaaa acttttgaat catcggacat aatatggata cctcctctat
    35101 tctatagtga acctaatatt cctaaatggg ctaagatgta tttggatgtg actgaaaatt
    35161 attatactct tgaagatgat gtcaaggttt ttctgatatc tattccgaaa gagaacagaa
    35221 gctttctttg gtagctattt atttttaatg agatttttca atacagcaat ctttttgcag
    35281 ttcttatctc aaaactaaaa caaaaaacta aagaaacgtg gaactccata gaggctgatc
    35341 gattctcaat agtctataat tgttgtctaa aactccgtcg acaagacctg cttaaaaatt
    35401 gaaaaatctc ttgacatcat aagggaaatc gtttaatata ccgaagtttt tccgcagtat
    35461 tcgtatccct taaaaatcta agggattatt tttttggagg taacaaagaa tgcctactat
    35521 taaccaatta gtaaggctcg ggagagaagt agtcaaaaag aagagttcat cgcctgctct
    35581 tttgaactgc ccacaaaaaa ggggagtttg cgtgagagtg tatactacaa ctcctaagaa
    35641 gccaaactcg gctctgagga aagttgcccg tgtaagactg acgaacggta ttgaggtgac
    35701 aacatacatt ccgggaattg gccataatct tcaggagcac tctgtggttc tcatcagggg
    35761 cggcagggtg aaagaccttc caggtgttcg gtatcacatc atcaggggct cacttgacgc
    35821 gagcggtgtt gccaacagga agaaaagcag atcaaaatac ggcgcaaaga agcccaaaca
    35881 gtaaggatag cattcaagga gcaacgatat gccgagaaag ggacatgttt caaaaagaga
    35941 aaaattgcct gatccaaaat ataatgatct ggtcgtggca aggcttatta accgtgttat
    36001 gctcgatggc aagaaaagca cagccagcag gattgtctac gaggcattcg atctcattga
    36061 gaacaaactg aaagaagaag ccttgaaggt gtttcacagg gctatggata atataaaacc
    36121 tgaattagaa gtaaaagcgc ggagggttgg aggtgcaaca tatcaggtcc ctgtagaggt
    36181 aaggctcaac aggaagcttt ctttagccat ccggtggatc atgaggtatg ccaaggaaag
    36241 gtcagaaaga acaatgactg agaagcttgc aggggagatt attgacgcat ataacaacaa
    36301 gggtggttct gtaaagaagc gcgaagatac ccacaaaatg gctgaagcaa acaaggcttt
    36361 tgcccactac agatggtaac actttcatga atcaaattgt aaggaacgtc gggattatgg
    36421 cccacattga tgcgggcaag acaacaacta cagaaaggat gttgttctac accggcgtca
    36481 ataacagaat aggggaagtc gatgatggca acgccaccat ggactggatg gagcaagaga
    36541 aggagcgggg tatcaccatt actgctgctg caacaacctg ttcctggaaa ggacacagaa
    36601 tcaacatcat cgatactccg ggtcatatcg attttacggt tgaagtcgga agatctctcc
    36661 gtgttctgga tggtgctgta gcgctttttt ctggtgttga aggggtagaa ccccagtcag
    36721 agacagtatg gcgtcaggct gaccgttaca gggttcccag gatagccttt gtgaacaaga
    36781 tggacagacc tgctgctgat tttcacaggt gtgtacggat gatcaaggat atcctcaagg
    36841 caaatccgct gatcctccaa attcctgtga aagatggaga tgaactcctt ggcaatgtcg
    36901 acattataac gcaaagggca acctattatg acaatgcaac aaggggtatc gagtacactg
    36961 ttaaagacat tcctgcccac ctgaaagacg aggcccttgt gttgagggaa cgtctcatgg
    37021 aatccctcgc cgagatagat gacggtttta tgactgctta ccttgatggt gctcttatag
    37081 aagaaactga aatcaagcgg attatcagga aaggtacctt gagcggcgct attcttccgg
    37141 ttttgtgtgg aagctcgttc aaaaacaagg gtgttcagcc gctcctcgat gcaattgtag
    37201 attatctccc gtcccctgca gatgtatctc cttatgaata ctggaccata gagggggaag
    37261 agggatattt gaaaggcacc cctgatgaac ctttcagcgg tcttgtgttc aagataatga
    37321 atgacccgtt tgtgggccag ctgagctaca tgaggatata ctcgggcgaa ctgaaaaccg
    37381 gagaaacagt acttaacaac tccaaaggaa ggacagagag aatcggtcga attatccggc
    37441 tccatgcgaa taaacgggaa gaagttaaga aaatacaggc gggtgatatc tgtgctatcg
    37501 ttggcctcaa aggagcatca accggtgaca cccttactgg ccaggacttt gaaatcctct
    37561 ttgagacgat agaggttcct gcaccggtcg tatccattgc tattgctccg aagaaaaagg
    37621 acgaccttaa gaagatgtgg cttgttttta accgctacac cgttgaagac ccatcgctca
    37681 atgtcaagct cgacagcgac acaggagagg tcatactctc cggcatgggc gaacttcatc
    37741 tggatatcat catggatcgt gctaggagag aacataacct cgatatgctg tcctctcctc
    37801 cccaggtagc gtaccgggaa accattacaa aatcagtgac aggcatagga aaatatataa
    37861 aacagtcggg tggtaagggc cagtatggcc acgtggtgct ccatgtatct cccattgaag
    37921 gtgctgattt tgtattcgaa aacaagattg ttgggggcgt catcccaagg gaatatattt
    37981 ccagcgtcga agccggggta aaggagagaa tggaaaaggg cgttctattt ggatacccgc
    38041 tggtcaacat taaagtggaa ctgatagatg gctcatacca cgaagtagac tcctctgaac
    38101 ttgcattcaa acttgccggt tctcttggtc tgaaagatgg cgtgacaaag gccaacccgg
    38161 ttattctcga gcccattatg aaggtcgata tcaatgtgcc tggcgactgc cttggccctg
    38221 ttatcggtga cctttcttca cggagaggga gaatagccga acttggcgac aggaatgact
    38281 tcaaatacat tgtggcccat atcccgctgg acgagatgtt tggttataca acccatctcc
    38341 ggtcagctac ccagggccgg ggttattata ctatggagtt tttccactac acccctgtac
    38401 cccagcatat ccttgagaat ttaatgaaaa atagagaaaa aacagtaacg gaggctctat
    38461 atggctaaga agaagtttga aagaaagaaa ccccatgtca ac
//
LOCUS       DAOOMC010000006        38374 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdus sp. isolate AMR_MDS_2685
            NODE_821_length_38374_cov_6.106083, whole genome shotgun sequence.
ACCESSION   DAOOMC010000006 DAOOMC010000000
VERSION     DAOOMC010000006.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864760
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdus sp. (wastewater metagenome)
  ORGANISM  Syntrophorhabdus sp.
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae; Syntrophorhabdus.
REFERENCE   1  (bases 1 to 38374)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 38374)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,729
            Genes (coding)                    :: 2,708
            CDSs (with protein)               :: 2,708
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 7 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38374
                     /organism="Syntrophorhabdus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_821_length_38374_cov_6.106083"
                     /isolate="AMR_MDS_2685"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2699756"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            645..3284
                     /locus_tag="PK125_01135"
     CDS             645..3284
                     /locus_tag="PK125_01135"
                     /inference="COORDINATES: protein motif:HMM:NF014758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HPB36741.1"
                     /translation="MEEITLNINGTPKRIIVDPKKTLLKVIREDLHLTGTKEGCSAGH
                     CGTCAVLVDGEVTMSCRYPVEKAKDKKIVTIEGIGTLENPHPIQLAFAANGAIQCGFC
                     TPGMIIRTKALLDKNPKPSRDEIAKAIQPHLCRCTGYIKVFEAIENAGAFLRKEIPSL
                     EIKTTGQIIGDEVPRRDSLAKATGTTLFVDDIPIDNCTYMKVVRSPHHHAKIISIDKV
                     QALSVPGVLAVFTAEDVKGTNILKMAGDDQPVLCGGKVRMIGDPVAAVVAITEKAAIE
                     GMNQVKVIYEELPSAITYEEALKDGAPQVHDGKPNVFFEQPIIFGDIEKGFSEADVVA
                     ERDFSTQIIEHGYLENDSGVAYIHENGQLVVMSGSQNIHQHKNTIAAAVGIDPARVRM
                     IQSVTGGAFGGKLDVSVGGLLGMAAIALQRPVKLVFTREETFAVTTKRHAFYMKAKVG
                     AKKDGTLTALQMDILADGGAYKSFSNSVVTRGLVHSSGPYRFLNAKIMGKAVYTNSAI
                     KGAMRGFGVPQVLFATESLLEEIAEKIGMDSLEIRKKNGYVSGDTTICAQELNDNFGF
                     HECLDQMRPHYERAVKEAKAASTDKVKRGVGLGCVFFGPGRSAPDQSEAWAELLPDDK
                     LQVWIGSADMGQGSDTMFLQIAAKTFGYPLNKVLICSTDTNFTPDGNFSAGSRQTYVS
                     GRAVQLVVEQLKKTMEENGCKTYADMKAKDIPTISKLVHHTTTTKLDPVDGHGVPWET
                     YSFGVQMAEVAVDVTTGKVDMVKITAVHDPGTVINKINVDGQTVGGIVQGIGYALYEE
                     YKYNETNSFAKFRMPRAKDIPPIEVYYVEVPRKNGPFGGSGMAEFCLVATVPAISNAV
                     YNACGARVCNLPITPDKVKKAMP"
     gene            complement(3717..4919)
                     /locus_tag="PK125_01140"
     CDS             complement(3717..4919)
                     /locus_tag="PK125_01140"
                     /inference="COORDINATES: protein motif:HMM:NF012766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HPB36742.1"
                     /translation="MLTNLSEGSFTPREELEDRINQLRKNMERAGIAFSIILQNVDLF
                     YFAGTVQKGVLVVSVDNGPLLFIERNVDRAAVESGLEIIPIKRDKDVGEILMAKGILK
                     GKCGMEFDVVPVSVFERWKSIVGLDDFTDIWPAIRDLRIIKSPFEIEQVKRSGSVCDH
                     VFEKARDVVREGAREVDIAAFLEAEGRRVGHQGFLRMRGLNQEMMNLYITHGRSGTIP
                     SSGDVPIAGAGVTHAIAQGPSLNKVEKGVPVVIDYGGGYNGYITDETRTFVVGELKER
                     FKKPYEVAKEIVEDATVYGKEGIDGTELFLRAEEKAKKAGLDEYFMGFESGKVTFIGH
                     GLGLEINELPVITPRHHIILKEGMVFAFEPKFIFPGEGAIGVEVDYVVRKDKLERVTR
                     TSIDLVTI"
     gene            complement(4894..6603)
                     /locus_tag="PK125_01145"
     CDS             complement(4894..6603)
                     /locus_tag="PK125_01145"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: protein motif:HMM:NF006625.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HPB36743.1"
                     /translation="MRYSKMFIPTMKEDPKEAEILSHRLMLRAGFIRKLASGVYTWLP
                     LGLKSLKKVENIIRQEMNRKGAQEIIMPFVQPRELWEESGRWEKYGKELLRIRDRNDR
                     ELCLGPTHEEVVTDLVRREVKSYRELPLILYQIQTKFRDEIRPRFGVMRSREFSMKDA
                     YSFDIDEAGAEKSYMDMYDAYMRIFERCGFNFKAVEADTGQIGGSFSHEFMVLADTGE
                     DTIISCGGCGYAANLERAEVGFVDREVPHTKGQLKDVETPGQRTVEEVSEFLGVKPDK
                     LVKTIIYNTDRGTMGVLVKGDREVNEVKLRNMLNLDVIELADEETIEKTTGGPLGFSG
                     PIGLSIPLYADRDVLFMEDFVVGGNVRDVHTVNVNNEDFKVEGYYDLKVAEPGDFCAR
                     CGGTYSSTRGIEVGHIFKLGLKYSKAMNCTFLDKGGKEIHAVMGCYGIGVGRTVAAAI
                     EQGHDENGMIMPFGIAPFEICVLPVNSSHAESMELAEAVYKELLYKGMDVIIDDRSER
                     PGVKFKDCDLIGIPIRVTIGERNLKDHMVEIKLRKEKDSQKVRKEEVIERVLNYANEF
                     KRR"
     gene            6857..7483
                     /locus_tag="PK125_01150"
     CDS             6857..7483
                     /locus_tag="PK125_01150"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HPB36744.1"
                     /translation="MGIQTNTCTVDTVLFDFGGVLAEKGFEEGLKTIALRNGLEEIPF
                     LSMVFDVIHATGYLTGHSDEHTFWKVIRDKTGIMEQDETMRNDILSRFIPRPWMFDIV
                     KKLKAAGVRVAILSDQTNWLDELDRKYDFFRLFDFVFNSYHVGKSKADPSHFVDTISR
                     LDKAPERLLFVDDTGAHCETARNLGINAIQFIGWESFIRDIRLFFPFL"
     gene            complement(7622..8452)
                     /locus_tag="PK125_01155"
     CDS             complement(7622..8452)
                     /locus_tag="PK125_01155"
                     /inference="COORDINATES: protein motif:HMM:NF012899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB36745.1"
                     /translation="MPLVREKDGRRLHVKSKLMGESLVSKSFIEGLPVAPQTRLYPDV
                     NILKVGGQSICDRGAKALPPIIQEIAVNKSEHKMLVTTGGGTRSRHIYAIGLELGMPT
                     GVLAKFGNSISEQNALLVTMLLAPYGGIKIGHDEIVKLTSYFVQGCIPVMHGMPPYDY
                     FAIPPAKGRIPIHRTDVGTIILADLIGARTCIFIKDEKGLYTGDPKKNAKEKFIPEIG
                     AKTLMEKDQDDLIVERPCLEILQNSEVLKQIQIVNGLEEGNITKALSGKKVGTIIYKE
                     "
     gene            complement(8520..8744)
                     /locus_tag="PK125_01160"
     CDS             complement(8520..8744)
                     /locus_tag="PK125_01160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB36746.1"
                     /translation="MAGKIFYREREKITEGKKQPRFKLVAVADVNLKVYGKHLRRSEL
                     EQIASSVGADLILLTHGDKKYKDEEIEVED"
     gene            complement(8701..9483)
                     /locus_tag="PK125_01165"
     CDS             complement(8701..9483)
                     /locus_tag="PK125_01165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB36747.1"
                     /translation="MIRIFVGFDDTDVKDSDRGTGKLARWFERELPRGCKVWGVVRQQ
                     LLVNELIPYTSHNSSACVVIECPETVSVEMIADRAISHIKHHFIEGSDPGLCICAEGN
                     PAMANLMVFGRVCAERVVSQKEALTAARGAHLSGHGGTNDGIIGASAAVGLTSQGWSG
                     RFIEFGRLRNIPESVEVGTLDQLGIKVISLDRDAQIPGPADIVKNDGWLRPRLVGGFP
                     VLLVEPIGPGLWKALEKKQNNGHREGTHNGRQNLLQGAGKDH"
     gene            complement(9485..10663)
                     /locus_tag="PK125_01170"
     CDS             complement(9485..10663)
                     /locus_tag="PK125_01170"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPB36748.1"
                     /translation="MSETSAFSVPGSDTQHGASNIQLPATDAILSARNLRVVRGGVTI
                     LDVPEFVVREGEVLCLIGPNGAGKTTLLQNICYLHKSFEGEVMFRGKIIGKDHGVFDY
                     RRRVAMVFQEPLLFDTTVFKNVASGLTFRGVDKKEIRKRVAENLERFGIAHLRDRSAR
                     TLSGGEAQRTSLARAFATKPEILFLDEPFASLDPPTREGLTRDLEKILLESQTTTISA
                     THDRNEALRLSDRIAFMRNGKIEQLGTPAELVNNPLDEFVASFVGVETVLTGNVVGKG
                     EGGTWIVSVSGKSIEVTEPLSEGESVVIHISPENVILSNNFVSKKTSLRNYFTARITC
                     IMPMGPFIKVYLDCGFPLVAYITNESKESLGLSEEKAVTASFKATAVQVIARKETEID
                     "
     gene            complement(10660..11358)
                     /locus_tag="PK125_01175"
     CDS             complement(10660..11358)
                     /locus_tag="PK125_01175"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPB36749.1"
                     /translation="MDLIFEGIIKAFWLLVTFDPEVMSIMFLSLKISGSATLVSLFIG
                     LSAGSALALTQFPGKKIVVGAVNTGMGLPPVVVGLFVSIFLWRSGPLGFLELLYTPTA
                     IIIAQSIIATPIVTGITLASLQQLPKKMRLQILALGASRAQMVWMLVKEAKLPLLAAV
                     MAGFGGVISEIGASIMVGGNIKGYSRVLTTATVMETSKGNFDIAIALSIILLFLAYLI
                     NLVLTYVQQRERPR"
     gene            complement(11475..12344)
                     /locus_tag="PK125_01180"
     CDS             complement(11475..12344)
                     /locus_tag="PK125_01180"
                     /inference="COORDINATES: protein motif:HMM:NF024257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HPB36750.1"
                     /translation="MKIKGFLKTVFVVTAVLIAFLGAQPAVDEAFAQQKNIILATTTS
                     TQDSGLLDVLIPVFEKKTGYFVKTIAVGSGQAMAMGQKGEADVLLVHSPAAEKKFVAD
                     GFGINRRLVMHNDFIVVGPGQDPAKIKGIKSSVDVFKKIASTGNLFISRADKSGTDVK
                     EKDIWKAGAIKYEGQKWYHETGLGMGQTLNIASEKKAYTLADRGTYLALKKRLDLDIL
                     AEGDAILLNIYHVIEVNPAKFPKVNAPGGKAFADFMVSKETQDMIKTFGVDKFGSPLF
                     FPDAGKKVEDLGK"
     gene            complement(12428..13294)
                     /locus_tag="PK125_01185"
     CDS             complement(12428..13294)
                     /locus_tag="PK125_01185"
                     /inference="COORDINATES: protein motif:HMM:NF024921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HPB36751.1"
                     /translation="MIRKVRFFAICLFMVIPILYGCGTTGDKFVFMASTIGPIDAGIV
                     DVLENQFEKETGIRVRHVGAGTGATLKIAEKGNIDLVMVHAKSLEEKFVKDGYGTQRF
                     DLMYNDFVIVGPPEDPAGIKGMKSAIDALKTISEKKATFISRGDKSGTHVAEVVLWEK
                     AGIKPSGNWYVVYEKGTQGNVATLKYTDQQKAYTVIDRASFLGLQKDIKTVILAEKDE
                     ALLNFITLIPVNPNKFPKVNHEPAMEFVKWLISPEKGQLIIRDFGKDKFGSPLFFPNS
                     KEWRESQGKKGN"
     gene            13513..14385
                     /locus_tag="PK125_01190"
     CDS             13513..14385
                     /locus_tag="PK125_01190"
                     /inference="COORDINATES: protein motif:HMM:NF024138.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HPB36752.1"
                     /translation="MQILSAKDLSKYLKINEKKIYKLAQESKLPYTKIGGKIAFTKEL
                     IDKWILENTSREHNIYLAGSDDILLRRIIDLYNQRRDGTVFYAPIGSINGLRLLKDSA
                     ATMSCVHILDVEKKNQDLAYLDRYLSKDDYIVVELYTREQGLILTKGNPKGIRGIEDI
                     ASKNVKFTNRNQGSGTRILVDFLLHEKNIDPGKIQGYALEADSHLEAGLKVLQGEAEA
                     TFGIRHVAHMLNLDFIHQFRERFDLVIPVDQYYYGHVKSFLAFFEQPTLLHNIRDFTG
                     YTITKIGSVIHKNV"
     gene            14378..14866
                     /locus_tag="PK125_01195"
     CDS             14378..14866
                     /locus_tag="PK125_01195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB36753.1"
                     /translation="MYEHIRRNIYNLFANRGVRVEVDETVDFPNFCCLKIQYQPEQNL
                     TLIIGKFTDDVLEILLIAHEFGHIMHYENLSREDAEAAYCAIFASNHLGLENISPESK
                     QLVVGIEKRASEYAISLLSVLGSDETILSRAKETYDEWIRGYLKKTGLSERVTILASS
                     HE"
     gene            14859..15683
                     /locus_tag="PK125_01200"
     CDS             14859..15683
                     /locus_tag="PK125_01200"
                     /inference="COORDINATES: protein motif:HMM:NF018076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HPB36754.1"
                     /translation="MSKTDLQNIIRVDILVKKGQEELLTDQIYQFINKGVWLEEQGNA
                     VLIKCYPEEPESFIQQVRVSGLKITGITTTQEKLQDYSELTRRYFRPITIGNFTIRAP
                     WNKKKPINRQIVIEPGMAFGTGRHESTKIMIKIMDSVEFSGKKVLDIGCGSGILSLNA
                     HLLGARRIYAVDNDLDATINAKKNVFLNQANTINLVCANLEDIKGTYDIVLANIDIKT
                     FAEHSERIVALVKEDGLLIISGILRKNRDQLLHLFHDWKLLKSHRIHSWMGFLFSR"
     gene            16253..17557
                     /gene="purB"
                     /locus_tag="PK125_01205"
     CDS             16253..17557
                     /gene="purB"
                     /locus_tag="PK125_01205"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HPB36755.1"
                     /translation="MIERYTLKRMADVWSDENRFRKWLEIEVLICEAYGSLSEIPPED
                     LKSIKEKANFNIDRINEIEKRTKHDVVAFIESVAEFVGPSSKYIHMGATSSDILDTSF
                     ACLLKEASEILIADIENFMTVLKEKAFEHKLTPMIGRTHGIHAEPITFGLKMAHFYDE
                     MRRNLERMKAAKERISHGKISGAVGTFAHLQPSIEEYVCKNLGLKPAPISTQIVPRDY
                     HAEFFTTLAIVASSVEKMSLEVRNLQRTEVGEVEEFFAKGQTGSSAMPHKRNPVASEN
                     LCGLARLLRGYALSALENIPLWHERDISHSSVERVIAPDATILLDYMLERVKNMYKNL
                     MVYPDRMEKNLSMSQGLHHSESILLSLIKKGLTRQEAYKLTQSVAMKCYDHGLDFRTE
                     LKRDQSIAQFLQESEIDDITTNEHYFKYVDTIFARVFIKKPL"
     gene            complement(17634..18491)
                     /gene="modA"
                     /locus_tag="PK125_01210"
     CDS             complement(17634..18491)
                     /gene="modA"
                     /locus_tag="PK125_01210"
                     /inference="COORDINATES: protein motif:HMM:NF024921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_function: GO:0030973 - molybdate ion binding [Evidence
                     IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HPB36756.1"
                     /translation="MKTRFLWPLVALLFVVMCFGNVFARDELMIFCGAAYKKPMDEII
                     QRYMKKNNIDVRVIYGGVGTLLSQITMSKRGDILMVPSSYIMEKAQSKGLVVPDSRKD
                     FAYVVPVINVRKGNLKGIRSLADLAKPSVRVAIANPEIVFVGMLAAEIVEKSLTREDI
                     KLLKKNIVTTPEDFNKLASALLLDKVDAIIGFHYLSSWYPDAIETIKLRPEEIQRIGA
                     GQIGILSHSKMMEQAKDFQRFLLSAEGQGVFAKYHYFGTPEEAARWVGDRKVVGGERP
                     ASTDWFGKE"
     gene            18946..19155
                     /locus_tag="PK125_01215"
     CDS             18946..19155
                     /locus_tag="PK125_01215"
                     /inference="COORDINATES: protein motif:HMM:TIGR00638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-binding protein"
                     /protein_id="HPB36757.1"
                     /translation="MKLSARNILKGRVKSVIPGVVNTEVKVEIAGGAEVVSVITKTSA
                     NSLGLVEGKDVYVVIKATSVMLAVD"
     gene            complement(19224..20303)
                     /locus_tag="PK125_01220"
     CDS             complement(19224..20303)
                     /locus_tag="PK125_01220"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPB36758.1"
                     /translation="MIKIESLETHLSGFNIYDINFSIEEGEFFILMGPTGAGKTVLLE
                     ALAGLIPVRHGKIFIAGKDVTRLPPEKRGVGIVYQDYVLFPHMTVTENIRYGLHFHKY
                     EHKETEKRFEELIEEFELKSLVNRLPLNLSGGELQRVALARALMVNPSVLLLDEPLSA
                     LDPNFREDIRHELKRIHQSSRTTFLMVTHDFAEALLLGNRGAVVNKGRIEQVGKMEDI
                     FQRPRTMFVADFVGMKNIYDAQFNGSKAIIGDGLELNIGCRSPVRVGRIAIRPDDIVI
                     TKKKYDKNNINAFSGIIAAVVDQGFYYEVHVQVQKLTFKSVVSKKILLESKFFDGAEV
                     FLSFEPEVIHIFQPDSYQFNEKGEA"
     gene            complement(20300..21085)
                     /locus_tag="PK125_01225"
     CDS             complement(20300..21085)
                     /locus_tag="PK125_01225"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPB36759.1"
                     /translation="MKRTELFFWVTVSCGLIVMVFILLPLIEMVTAPSISMLVMAIRD
                     KDVVRSIWLSVYTAGLAALISFVVGTPLAYVLARRDFRGKRLVEGIIDLPIVIPHPVV
                     GIAILGVVGKNHWIGRGLAELGVRVMGSVTGIVTVLIFVGLPFYVHAAKEGFENISPR
                     LERVSRSLGASMFSTFFRITFPLAWRSMVVGIIMCAARAISEFGAVVVVAYHPMIAPV
                     LIYERFEGYGLKYSQPVAVWLVMVCLSLFFVLRYLTLPNKKKT"
     gene            complement(21130..22155)
                     /locus_tag="PK125_01230"
     CDS             complement(21130..22155)
                     /locus_tag="PK125_01230"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPB36760.1"
                     /translation="MIELTDINGDLGEFCLRDINLTVNEGEYLALLGPTGAGKTVLIE
                     YIAGIHNPNKGSIKVDGEDITNLFMEDRNIGYVPQDYALFPNLTVEKNISYGLEARRM
                     QPEQLNSIVSEMITRLEIDYIKHRMPLHLSGGEKQRVALGRALATKPKVLLLDEPLSA
                     LDENLRTEMARELRQIQRDSNGTFIHVCHNFEEAADVADRVAIMDNGRIIQVGTLKEI
                     MEYPKNEFVARFLKSQNIFKGVSDGSVLNIENIVLVKKNDCIGNVVVAVRPENITIVR
                     PDDGKESNIFQGKVRGSVLKPYFQDVCVDIGISVVMYTMDDREFNEGDEVKVRIPPEK
                     IIVMGES"
     gene            complement(22166..23086)
                     /locus_tag="PK125_01235"
     CDS             complement(22166..23086)
                     /locus_tag="PK125_01235"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HPB36761.1"
                     /translation="MSGKTPRRKSFNMLFYSFAGFLFLYSAYLFLSNVDQLFVTETAV
                     QTGGITAVWGEDREVLNKFAGGFWNDPYFWKSLNLTVVSTLITTLIASVLGIPTAYVL
                     SRYRIPGISAIEVLFSSLIVMPGSVIGICLIVMFNYGPLWNLQNAVGFKFAHSLFPGM
                     VLASLVLSFALGLSAWKAAFDSVNPRFEHVARSLGSSRWRAFRTVTLPLAKSGIIAGI
                     ILAWTRAMAEFSAVLFFCGTFRELPPSRFSNFTKLIKMDQADWVSVAVWAQVEFGNVE
                     YGFALAYVLVVIGGISVYVMHRIGAKGYVW"
     gene            complement(23076..23876)
                     /locus_tag="PK125_01240"
     CDS             complement(23076..23876)
                     /locus_tag="PK125_01240"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPB36762.1"
                     /translation="MAAKARGGWWPTLSVGITITILFAYVLMVLSNWIYLGWSDTVYF
                     FSKSRMWERFWLTVWTSLASTFFSLCIGIPAGYALSRFTFPYRNLIASIIDLPIVVSP
                     AVVGAFLFGITTRFPFDLISEHYGIYIGRNVYGVLLVQFTVTAAFCARLMKASFDMIP
                     TRFEAVSRSLGASPLRTFFRVVLPMAKNGIIASMIVVWARAAAEWEGLMLFVGATEGK
                     TDIMPFAIYLDFNAGMMGWVTSMSIVCILMAISAISAMKMIGGRSNVW"
     gene            complement(23901..24854)
                     /gene="modA"
                     /locus_tag="PK125_01245"
     CDS             complement(23901..24854)
                     /gene="modA"
                     /locus_tag="PK125_01245"
                     /inference="COORDINATES: protein motif:HMM:NF024921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_function: GO:0030973 - molybdate ion binding [Evidence
                     IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HPB36763.1"
                     /translation="MKRILRIFVFLALFSGFLVPQVNGGEKALLFHAGLGQRSALNEI
                     KNVFEKKHPEIKVNFSYKGSGYFIADITRSKQGDLFMPGEEFYLMQAVERGFVTDYNP
                     KTDIPAYFVTVIITPKGNPKNIKSIDDFAKPGVKVGLGNPKSCAIGIWHEKTFKKAGI
                     WDKVKANATLSAKCIPELGNATQHKAIDATIVWGTTAVLYLRDVEIIPIEYKYRGIVR
                     LPVGILKFARYKNEARELMNFILSDEGRHIFHKHAYTVNKVIPVDKNGFCLDGTTDKD
                     MGYLVNAAKAVKDESFPVNTETVGDLIDEVMRQRKTTRAGD"
     gene            complement(24958..25926)
                     /gene="wtpA"
                     /locus_tag="PK125_01250"
     CDS             complement(24958..25926)
                     /gene="wtpA"
                     /locus_tag="PK125_01250"
                     /inference="COORDINATES: protein motif:HMM:NF003196.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tungstate ABC transporter substrate-binding
                     protein WtpA"
                     /protein_id="HPB36764.1"
                     /translation="MKKATVIVCFAVMLLVSVQVFAEPQGKLVMFHAGSLSVPFEAME
                     KAFEAKYPKVDLQREAAGSQACARKITDLKKPCDIMASADYKVIDKLLIPGSADWNIR
                     FATNQLVLCYTDKSKFAKEVNANNWYEILQKKGVVWGHSDPNLDPCGYRSLMVLQLAE
                     KYYKMPGLYDKMIANRPKENVRPKSVELVSLLQTGNMDYAWEYLSVAVQHGLKYVILP
                     DEINLGNHKYDNFYQQAVVRVTGKEPGSFMDMKGDSVTFGITMIKDAPNKEAAIAFLK
                     YMLDPQGGLKILKEQGQPPFVPCRVSTDKMKALVPVELQGLVEVKD"
     gene            complement(26310..26729)
                     /locus_tag="PK125_01255"
     CDS             complement(26310..26729)
                     /locus_tag="PK125_01255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB36765.1"
                     /translation="MLLRDGDCENALQYLSIAADRNPQQGEVYGYMGVAFFILGMWTE
                     AMDSYQRALSLDSSLEKVYFFRGVLHSQLAHYKEAIDDFTIAIEWEHLFDDAYCYRGA
                     CRVFIDDYKSAIQDIRFAARLGNKIAQKMLNLMNIPW"
     gene            complement(26803..27225)
                     /locus_tag="PK125_01260"
     CDS             complement(26803..27225)
                     /locus_tag="PK125_01260"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(deoxy)nucleoside triphosphate
                     pyrophosphohydrolase"
                     /protein_id="HPB36766.1"
                     /translation="MKKKKVNPQVVTVAVIERNGHVLIAKRKRGKMRAGNWEFPGGTL
                     EEGETYKECLKRELKEELTVTSEIGDLICSNEYRYASDWTVKLMAYKAAIVSGSLNLH
                     DHEDLRWVKPEDLIDYEFPEVDRPIIEWLVSKNGKPSK"
     gene            27537..30218
                     /gene="fdhF"
                     /locus_tag="PK125_01265"
     CDS             27537..30218
                     /gene="fdhF"
                     /locus_tag="PK125_01265"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013706032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:28587..28589,aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
                     /protein_id="HPB36767.1"
                     /translation="MKITLDGTVYEAEPGQTILEVARANGINIPTLCYMPGLPPFGAC
                     RICLVEVERSPALVAACTAPVMEGMNIKTNSKRVIDARRFVVQLLVSCHPLDCMTCEK
                     TGDCTLQDLAYELGVTNPREDEGDRHKYPIDSSNPFIIRDYNKCILCQRCVRVCDEIQ
                     GIVAIKNINRGFDTKIATAYDGTLQDSNCVFCGQCVQACPVGALVPKKAVGKGRHWEL
                     QKVRTTCPYCGVGCQLNLHMKGDQIAYVSAVEDGSPNKGRLCVKGRFGYDFIYSEDRL
                     KTPLIKENGAFREASWDEALDLVASKFKQIISESGPDAIAGVSCARSINEDSYQMQKL
                     FRAVIGTNNIDHCARTUHAPTVAGLAQSFGSGAMTNSFGEFADAKMFLCIGTNMTEAH
                     PVAATFLKNALRKGAKLIVVDPRKHELAGLADIHCQIKVGTDIAFLNGLMNVLITEEL
                     YDKDFVQNNCDNFEVFRTKILEYPLERASAISGVDAETIRQAAHMLAEIKPAMLCYTL
                     GITEHTTGTDNVLSTANLQLLLGNMGVPSGGVNPLRGQNNVQGACDMGALPNVFPGYQ
                     KVIDESVRAKFAKAWGVKNLPDQNGLMMSQMMEGLVTKKIRAFYIFGENLANTEPDIH
                     KTEHELASAEFLVCQDIFPTETTRFAHVVFPAAAWSEDDGTFSCSERRISRVRKVKNP
                     PGQAKPNWWIFKELARRMGQEWQSNSSQDIWDNEVSVLAPAFGGIKYNRIEKDGLQWP
                     CPNIDHPGTQFLHKYGVFTHGKGIFSPVDWKPPAEVPDEEYPFVLSTGRRLFHYHTRT
                     QTGRTGLDFLLSEETADISLADAQKLGIQDGEYIKVRSRRGNVTVKARVTPEVPPGMV
                     WMAFHFRDACANWLTNAVYDPVTFTAEYKACAVAIER"
     gene            30799..33459
                     /locus_tag="PK125_01270"
     CDS             30799..33459
                     /locus_tag="PK125_01270"
                     /inference="COORDINATES: protein motif:HMM:NF014758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HPB36768.1"
                     /translation="MKKVVMKINGKLHQFVVEPDRVLLDLLREDMLLTGAKQSCDRKG
                     QCGACTVIVNGQAVRSCLQKVANLDGAEVITIEGLGTPENPHLIQEAYVLSGAVQCGF
                     CTPGMIMATKALLDRNPNPNVEEIKKALEHNLCRCTGYIKIIEAVQLAARFIRGETTP
                     GAVRPDPNGPKIGVSHPRPSAMLKACGVAPFSADIKLQDALELAVVRSTEHHANIVSI
                     DTSKAEKMPGVVGVITANHIKGTNRIVIIFPDEPILCKDKVQCLGDPIAIVAAQTKQQ
                     AIAAAEAVKVTYEPLPVLRSPQEAMAEGAIQIHKEWPNLCFVQPQIKGDAEKAIAESA
                     AVVEASFTTQINHQAPLEPEATVAYMEGEGEDAQLVVVGRSIQIHGVMTNLQEALGYE
                     NIRYEEAYSGGQFGIKAAITTEGISAAAALHLQRPVRYIPSIAESMLLTNKRHPFDMK
                     VKLAADENGKLTAFTIDYIVDNGAYQIIGIYVIMRAMWMLSGSYNIPNVNAMGKLVYT
                     NNPAGGAARGAGPPQVTFALESAMDMLAEKIGMDPLRFRKINSLLPGQSVSTGMVYEQ
                     WPFPELCDAIQAKYDSAKKEAATLKSGPIKRGVGIATHAFGIGGPGDAGRVAVELDPD
                     DGITIYAAVADPGEGNDSMLTQIASHLTGIPMDKVRLVTRDTDHTAEMGPAAASRMTY
                     MAGGSLVLAIEQLKKAMEEAGVKTYEGLTKAGKPIHYVGSKKAAGQEGPLDPVTGQGP
                     SFESRVHNIQMAEVDVNTDTGEVQVIKMTTAVDAGTVINPQNLEGQLQGGMDQGVGYA
                     LREEYVHGKTRDWVTFKFPTMKTSFPTDIIIRETPRVKGPLGATGIGEMTMVSTAPAI
                     INAIYDACGVRIFDLPATPEKIKAALAAKK"
     gene            33542..33907
                     /locus_tag="PK125_01275"
     CDS             33542..33907
                     /locus_tag="PK125_01275"
                     /inference="COORDINATES: protein motif:HMM:TIGR03342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TusE/DsrC/DsvC family sulfur relay protein"
                     /protein_id="HPB36769.1"
                     /translation="MNDSATHTLEAGDVHQKFRTIAGREILFDKQGFLWHPEDWSEEV
                     AVALALESGIEALSNDQWRVIRFLRDYFAYHGRAPLNRDLKAGLNISLMNLESLFPGG
                     IRHGARRIAGLPNPRACTG"
     gene            33913..35079
                     /locus_tag="PK125_01280"
     CDS             33913..35079
                     /locus_tag="PK125_01280"
                     /inference="COORDINATES: protein motif:HMM:TIGR01977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HPB36770.1"
                     /translation="MLQRLIYLDNAATTFPKPASVLNRMVEIYLQMGVSPGRGSYDMA
                     VEAEEMVTNTRQKLAHFFGAPDPDRVIFTNNATDALNLLLQGMLKPGDHVVSTRLEHN
                     SVLRPLNHLHMKGIIEYDLVPFDANGFVDPGDILRSIRTNTKLVIMCHASNVLGTIQP
                     VQEVGRLCSERGVPLFIDAAQTAGLIPINMKSWFLAGVAFTGHKSMLGPTGIGGLVLN
                     QEVEIEPTHFGGTGVDSKSLVHTPSFPHRLEAGTLNLLGIIGISESLAFLLHEGIEAI
                     HRREMDLLHRLHDGFSHFKTINIYGPKNLSDHVGLITVNVEGMSPEDVGSILDADFGI
                     AVREGLHCAPLVHESLGTSPKGGVRFSLGPFTTEEDIDATLAAMATIARTKDCC"
     gene            35130..36113
                     /locus_tag="PK125_01285"
     CDS             35130..36113
                     /locus_tag="PK125_01285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB36771.1"
                     /translation="MKQREISKSDPYVPNDSKNLYVNMEGWSHKYKGPAAKKAILHHL
                     EHWPQARALAAEICDFYGNCLLYYEDSNLESSHYQINLKHDPIVFNHACVQLQLGTSR
                     TAEALCHELLHLSTRMCGFPFGEKFFIPHDLIDYAATYMGFYPVIGNLVEHELIFEKF
                     IDIGFDKRNFLGCISPPPDYKTLASTAVKSSFYRKEIGFPWWCLEYFRHWISIRHWIG
                     DEPALYADCALFWGSKVHPSMKATTRNIRDLIESGVLTDRKQYHRHVNTLLELMKIPK
                     FTEWTFIKSGKDSTPLVYRLAHKEKINPNGSNSHVLKTMGTTLKHGFQSNL"
     gene            36336..37541
                     /locus_tag="PK125_01290"
     CDS             36336..37541
                     /locus_tag="PK125_01290"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012336.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006635 - fatty acid beta-oxidation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HPB36772.1"
                     /translation="MKSFTKVFIPYGGYYSTPFCRWQGSMQHDNAVFLGPNTARRWFL
                     EKRKIDPTVIDYLYFGTTVAQHHWFFSHQMAGAIITDDKKFVPGLFIHQACSTSTTIV
                     NLAAQNIELGTYEVCFGLMSDRCSNGPHTTWANPLGPGGEPYREDWNMDNFGGNPRVP
                     VAMVETAENVAREAGITKEECDAVVLRRFEQYQDALANDRAFQKRYMFPALCTVSRKK
                     TKLVEEDEGWTPTTAEGLASLKPVRPGGVHSFGSQTFPADGNCGIIVTTRDKAKELST
                     DPKIEVQVISYGAARVEPAHMPAAPVPAAQMALDMAGLKITDMKAIKTHNPFSTNDIN
                     LAKKMGIDVMKFNNYGSSLIYGHPQGPTAGRGIIEMIEELVLLGGGYGLFTGCAAGDT
                     AAALIVKVG"
     gene            37678..>38374
                     /locus_tag="PK125_01295"
     CDS             37678..>38374
                     /locus_tag="PK125_01295"
                     /inference="COORDINATES: protein motif:HMM:NF014757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HPB36773.1"
                     /translation="MDIKTIGVLGAGSMGNGIAQVAAQSGYNVVMRDIDDKFVDRALK
                     GIDKFLSKSVEKGKITSDDKSATLGRIKGTTKMEDLKDVDFVVEVVLEDMDLKKKVFK
                     ELDELTRKEVILATNTSSMSITEIATATNRQDKVVGMHFFNPVPLMRLVEVIRGYNTS
                     DDTIAVAMDLARKFKKEPIEVKKDIPCFVVNRLMMPHFLEAILLLQEGIASAEDIDKA
                     AKLGLNYPMGPFEL"
BASE COUNT        10764 a         9089 c         8099 g        10422 t
ORIGIN      
        1 tgtagccaca ttatatcttt tgaagattgt ccagtcaagt cacaataagt tttatttagt
       61 tagtaatttt aataatttgt cattatatat tatttctgcc gtgatcagta atgggtaaag
      121 ggtaaaaaaa atagattact gattatgcgt cacgcaccat ggattctggc cccagggttt
      181 taggtttcag ggacgttctt gaaatgttga aatgtttatt gccagctagt atgctaaata
      241 acccacaaac tatcaaacta acagcttggt aattatggga ctgcaccgac tctgcgtaga
      301 aacgcagtga tttcctgtgc aagaaaacgg tatccttccc cgctttgatg aatctggccc
      361 gtctttaacg tgctcatcgc cggcatgtct gacagtaacg atgcttccaa tgataccttt
      421 tttgctcgtc atccctatcc gatattatca ccatagaaaa aaactccatg gtgttcccag
      481 aactgctcat aacctgtttt ttcacaaaat aactataagt tatttttagt aattcgtaaa
      541 actatttgta ttgactgacc ttaacaacca tgattactta agttagatct ctgtgcatga
      601 gacagagact ttccgtaccg cattaatcaa aggagggagg aagcatggag gagattacac
      661 tgaacattaa cgggacccct aagcggatca ttgtagatcc gaaaaaaaca ctattaaagg
      721 taattcgtga ggatcttcac ctaacaggaa caaaggaggg ttgttcagcc ggacattgcg
      781 ggacctgcgc agttctcgtt gacggagagg ttactatgtc gtgcaggtac ccagtagaaa
      841 aagcaaaaga caagaagata gtgaccatcg aaggcatagg cactcttgaa aacccgcatc
      901 ctattcagct tgcttttgct gcaaacgggg caatccagtg cggtttctgt acacctggca
      961 tgattatcag aaccaaggca ctgcttgata aaaatccaaa accatcacgt gatgaaattg
     1021 cgaaggctat tcagcctcat ctctgccgat gtaccggata tattaaggtc tttgaggcca
     1081 tagaaaatgc aggcgcattt cttcgcaaag agattccgtc cctggaaata aagacaaccg
     1141 gacagatcat tggtgacgag gttccgcgtc gggattctct tgcaaaagca accggaacaa
     1201 cgctctttgt tgatgatatc cccattgaca attgcaccta catgaaggtg gtaaggagcc
     1261 cccatcacca cgctaaaatc ataagcatcg ataaggttca ggcactctcg gtgcctggtg
     1321 tcctagctgt attcaccgcg gaagatgtca agggaacaaa catcctgaag atggctggtg
     1381 atgaccaacc tgtgctgtgc ggaggcaagg tgcgcatgat aggcgatcct gttgctgctg
     1441 ttgttgcaat cacagaaaag gcagcgattg agggtatgaa ccaggtcaag gttatctacg
     1501 aggaattgcc gtcagcgatc acatatgaag aggcccttaa agatggggca ccacaggtac
     1561 acgatggtaa gccgaatgtg ttctttgaac aaccgattat atttggtgac attgaaaagg
     1621 gtttttcaga agcagatgtg gtggcagagc gtgatttctc tactcaaatc atcgagcatg
     1681 ggtaccttga aaatgattct ggagtagcct acatccatga aaatggccag cttgtggtca
     1741 tgtcaggaag ccagaacatc catcagcaca agaacacaat tgcagctgcg gtggggattg
     1801 atccagcaag ggtaagaatg atccaatccg ttactggcgg tgcgtttggt gggaagcttg
     1861 acgtatcagt cggtggtctc ctaggtatgg ctgccattgc ccttcaaagg ccggtaaagc
     1921 tcgtgttcac ccgagaggag acatttgcag ttacaacgaa acgacatgcc ttttacatga
     1981 aagccaaggt aggtgctaag aaagatggca ccctaacagc cctccagatg gatattcttg
     2041 ctgacggagg tgcatacaaa tcgttctcaa acagcgtagt gacccggggt ctggtccact
     2101 caagcggtcc atacaggttt ttgaacgcca agataatggg aaaagctgtg tacactaact
     2161 ctgccatcaa aggagccatg cgtgggtttg gggttcccca ggtactcttt gccactgaat
     2221 cgctccttga agaaattgca gaaaagatag ggatggattc cctcgaaatc agaaaaaaga
     2281 atgggtatgt ttcgggtgat acaacaatct gtgcccaaga attgaatgat aactttggtt
     2341 ttcatgaatg tttagaccag atgagacccc actatgaaag ggcagtaaag gaagcaaagg
     2401 ctgcatcaac cgacaaagtg aaacgcggtg tgggccttgg ttgtgtgttc tttggcccgg
     2461 gaaggagtgc ccctgatcaa tcagaggcat gggcagaact tctacctgac gataagctcc
     2521 aagtatggat aggttcagct gatatgggcc agggatcaga cacgatgttt ctccagatag
     2581 cagcaaaaac attcggttat cctctcaaca aggtactgat ttgctcaaca gacacgaatt
     2641 ttacgcctga cggtaatttc tccgcaggaa gccgccagac ctatgtatca ggcagggctg
     2701 tccaactggt tgtggaacag ttaaaaaaaa caatggaaga gaacggatgc aagacctatg
     2761 ctgacatgaa ggcaaaggat ataccaacca tttcaaaact tgtacaccat accacaacaa
     2821 caaaacttga tcctgtcgat ggccatggag tgccctggga gacatattcc tttggcgttc
     2881 agatggcaga ggtggcggtg gatgtcacca caggcaaggt cgatatggtc aaaatcacag
     2941 cggtgcacga tcctggtacg gtaattaaca agatcaacgt ggatggtcag actgttggcg
     3001 gaattgtcca gggcattgga tacgcactat acgaagaata caagtataac gaaacgaata
     3061 gctttgcaaa atttagaatg ccaagggcaa aggatattcc gccaattgag gtttactacg
     3121 ttgaggttcc aaggaagaat gggccgttcg gtggatctgg aatggcagag ttctgccttg
     3181 tagcaacagt tccggcaatc tcaaacgcag tttataatgc ttgcggggca agggtttgca
     3241 acttgcccat aacccctgac aaggtaaaaa aagcaatgcc atgaccagcg tttgccccct
     3301 ataagcagaa ggctcccggc attcagggca gccttctgcc cccattgagc cgaattgcaa
     3361 tttaataaat attttaactt ttttacgtga cacctgcagc ttttctgagg aaatcagcta
     3421 tgccctgcca acgaaagcgg tattcttttt cgtttatgta catggtctgc ttttaacttg
     3481 ttgcttgtca aaatgtatgg cagcaataaa ccttccaatg gtacccccgt gtctttgggg
     3541 atttaccgta gaatgagcta tcaattggat tcacccccac accagatgtg atgctcttac
     3601 cagacgcaag aataacgaca tcctcagaaa cccttgatag cctgggctgg gcgttgttgc
     3661 agacagcgtg gataatgaaa agagttgtat aaacaaaatg tttccgctgc acaatgttag
     3721 attgtaacca ggtcgatgga ggttctggtt acacgctcaa gcttatcttt ccgaacaaca
     3781 tagtccacct caaccccgat ggccccttct ccgggaaaga taaactttgg ctcgaatgcg
     3841 aaaaccatac cttctttcag gataatatgg tgtcgagggg ttataaccgg aagttcattg
     3901 atttcaaggc cgaggccatg accgataaat gtcaccttcc cagattcaaa gcccatgaaa
     3961 tactcgtcga gtccggcttt tttcgccttt tcctctgccc ttaagaaaag ctctgttccg
     4021 tcgatgccct ctttgccata taccgtggca tcttccacaa tctctttggc gacttcgtat
     4081 ggctttttga accgctcttt caactcgccc accacaaaag ttctcgtctc gtctgtaata
     4141 taaccgttat aaccaccacc gtaatcgata acaacaggca cacccttctc tactttgttg
     4201 agagatggtc cctgggcaat ggcgtgggta acacccgccc cagctatggg tacatccccg
     4261 ctcgacggta tggtgcctga ccgtccgtgg gtaatgtaga gattcatcat ctcctggttc
     4321 agcccccgca tcctcaaaaa accctgatgt ccaaccctgc gaccttccgc ttccaaaaag
     4381 gcagctatgt caacctccct ggccccttca cgaaccacat ccctagcctt ttcaaaaaca
     4441 tgatcgcaca cactcccaga tcttttcacc tgctcaattt caaaggggct tttgataatt
     4501 ctgaggtccc tgattgcagg ccatatatcc gtgaagtcat caaggccaac gatgctcttc
     4561 catcgctcaa agacagagac aggcacaaca tcaaactcca ttccgcattt gcctttcagt
     4621 atccctttcg ccatcaggat ttcacccacg tccttgtccc gcttgattgg gataatctca
     4681 agaccggact caacagcagc tctgtcgaca tttctttcaa tgaaaagcag tggtccgtta
     4741 tcaacggaca caaccagcac acctttctgg actgtgccgg caaagtaaaa cagatcaaca
     4801 ttctgcagaa ttatggaaaa agctatgcct gcccgctcca tatttttcct caactgatta
     4861 attcgatcct ctaactcttc cctgggagtg aaactacctt cgcttaaatt cgttagcata
     4921 atttaaaacc ctctctatta cttcttcttt tcgaactttc tgagaatctt tttcttttct
     4981 cagttttatc tctaccatgt gatccttcag gtttctctcg ccaatggtga cccttatggg
     5041 tatgccgatg agatcacaat ctttgaattt gaccccgggc cgttccgacc tgtcatctat
     5101 gataacatcc atacccttat aaagcaattc tttatagaca gcctctgcca attccatact
     5161 ctcagcatgc gagctgttca ccggtaaaac acaaatctca aaaggagcaa tgccaaaggg
     5221 catgatcatg ccattctcat cgtgcccttg ctctatggct gccgctacag ttctacccac
     5281 cccaatgccg tagcaaccca tgactgcgtg tatttctttt cctcccttat ccaggaatgt
     5341 gcagttcatg gctttgctgt acttgagacc aagcttgaag atgtggccta cctcgattcc
     5401 tcttgttgaa gagtaggtgc ccccgcacct ggcacagaag tcgcctggtt cggctacctt
     5461 caggtcatag tatccttcca ctttgaagtc ttcattgttc acgttaaccg tgtgcacatc
     5521 ccgtacattc ccacccacaa caaagtcctc cataaaaagg acatcacgat cagcgtatag
     5581 ggggatgctc aggccaatag gccctgagaa gcccaaaggt cctcctgttg tcttctcgat
     5641 tgtctcttca tcagcaagct ctataacatc gaggttgagc atgttgcgga gttttacctc
     5701 attaacttcc ctgtcgcctt taacaaggac ccccatggtt cccctgtctg tattgtatat
     5761 aatggtctta acaagtttgt ccggtttaac acccagaaac tccgacacct cttcaaccgt
     5821 tctctgaccg ggagtttcca catccttaag ctgccccttt gtgtgtggca cctccctgtc
     5881 aacaaagcca acctctgcac gttccaggtt agctgcatac ccgcagcctc cacaagagat
     5941 aatggtatcc tcgccggtat cagcaaggac catgaactcg tgggaaaaac tccctccaat
     6001 ctggccagta tccgcctcca cagccttgaa attgaatcca catcgttcaa atatcctcat
     6061 gtaagcatcg tacatgtcca tgtagctttt ttccgcacca gcctcatcta tatcaaagct
     6121 gtaggcatcc ttcatagaga attcccgtga acgcatgacc ccgaatctcg gccgtatctc
     6181 atcccgaaac tttgtttgta tttggtatag gattagggga agctcgcggt aagacttaac
     6241 ctcgcgacgg accaggtcag tgacaacctc ttcatgggta ggaccgaggc acaattctct
     6301 gtcatttctg tcgcggatac gcaggagttc tttgccatat ttttcccacc ttccactctc
     6361 ttcccagagt tccctgggct gtacaaaggg catgataatc tcctgtgcac ccttgcggtt
     6421 catctcctgg cggattatgt tttccacctt tttgagggat ttgagaccca ggggcagcca
     6481 agtatagact cctgacgcga gcttccgtat aaatcctgcc cgtagcatga ggcggtggct
     6541 taaaatctcg gcttctttcg ggtcttcctt catggtagga atgaacatct ttgaatagcg
     6601 cataatatct cctgctataa actgttaatg attagcgatc agctttcaga gacaaatcca
     6661 aaaagccaaa ccaatttaaa ctgatttttt gctggcaact taatgttgat agctgaccgc
     6721 aaccatcgaa aggttacttc aaaacataag gattttcaag ggctttattt gcttcggata
     6781 tcaactactg cagcaccatg aaaagggtgg aaaatagttt tcacgatatg ctacattctg
     6841 aattcaggag attgacgtgg gtattcaaac caatacatgt accgtagata ctgttctttt
     6901 cgattttggg ggtgttctgg ctgaaaaagg atttgaagaa gggcttaaaa ccatagccct
     6961 cagaaacgga ttggaggaga ttcctttcct ctctatggtt tttgacgtaa tccatgccac
     7021 aggttacctc accggtcatt ctgacgaaca tacattctgg aaggtaatcc gtgacaaaac
     7081 aggaatcatg gaacaggacg aaactatgcg aaacgatatc ctttcccgct tcatccccag
     7141 gccatggatg ttcgatatcg taaaaaaact caaagctgca ggtgttcggg ttgccattct
     7201 cagtgaccag accaactggc tggatgaact tgataggaag tacgatttct tcagactctt
     7261 cgatttcgtt tttaacagct atcatgtcgg gaaaagcaaa gctgatcctt ctcattttgt
     7321 ggatacaatt tctcgtcttg ataaagcgcc tgagagactc ctttttgtgg atgataccgg
     7381 ggcacattgc gagacagcgc gcaacctggg cattaatgcg attcagttta ttggttggga
     7441 gtctttcatt agagatattc ggcttttttt cccgtttttg tgatcttatt gtcaccttga
     7501 agcccggaag aatctttatt tgccactgta gtagcgattt ctggcgtccg ctgttgacac
     7561 actgcaataa agaatgtaca ggctatcttg caatatcatg ttacgagcag ggcgagggct
     7621 gttattcttt atagatgatt gtcccgacct ttttgccgga aagggctttt gtaatgttgc
     7681 cttcttcaag accgtttaca atctggattt gtttcaagac ttcactgttt tgaagaattt
     7741 cgaggcatgg gcgttcaacg ataagatcat cctgatcctt ttccataagg gttttggctc
     7801 caatctcagg aataaatttc tcctttgcgt tcttttttgg atcacccgtg taaaggcctt
     7861 tttcatcctt gataaatatg cacgttcgtg caccaataag atcggccagt atgatagtgc
     7921 ccacatctgt tcgatggatg gggattcttc cttttgctgg gggaatagca aaataatcgt
     7981 atggaggcat gccgtgcatg acagggatac aaccctgaac aaagtagctc gtaagcttta
     8041 caatctcatc gtgtccgatt tttattccac cataaggagc gaggagcatt gttacaagca
     8101 gtgcgttttg ctcggaaata gaatttccaa attttgcaag cactccagtt ggcatgccca
     8161 gctccagccc tatagcataa atgtgtctgc tgcgggttcc tccgcccgtt gtgacaagca
     8221 tcttgtgttc tgacttgttc acagcaatct cctggataat gggcggcagg gccttggcgc
     8281 ctcgatcaca tatggattgc ccccccacct tcaatatatt tacgtctgga taaagtcttg
     8341 tttgcggagc taccggaagg ccttcgatga atgatttgct caccaggctc tctcccatca
     8401 acttgctctt cacatgaagg cggcgaccat ctttttcacg aaccaatggc ataaagatac
     8461 tccttgtcga taagtttgaa accggctaaa aggaacgtat tgcgtttggg tagaactgtc
     8521 taatcctcta cttctatttc ctcgtcctta tactttttgt ctccatgggt caggagtatg
     8581 aggtcagccc caaccgatga agcaatttgt tcaagctcgc tccttctgag gtgttttccg
     8641 taaaccttca ggttcacatc ggcaacagct acaagtttga accttggctg tttcttgcct
     8701 tcagtgatct tttcccgctc cctgtaaaag attttgcctg ccattgtgtg ttccctccct
     8761 gtgcccatta ttctgttttt tttcaagggc cttccataag cctggcccta tcggttccac
     8821 caatagaacc ggaaaccctc ccacaagtct cggccttagc cagccatcgt tcttgacaat
     8881 atcagccgga ccaggtatct gagcatccct gtctaaagag atcactttta ttccaagctg
     8941 atcaagcgtg ccaacttcca cagattcagg aatatttcgc aatctcccaa actctataaa
     9001 tctaccgctc caaccttgag atgtcaaccc aaccgctgcc gacgctccaa tgataccatc
     9061 attggtgcct ccatgcccgg aaagatgagc ccctctggca gctgtaaggg cttctttctg
     9121 ggagacaaca cgctcagcgc agaccctgcc aaaaaccatg aggttggcca ttgcagggtt
     9181 accctcagcg cagatacaca gaccgggatc actaccttca ataaagtggt gttttatatg
     9241 agagatggcc ctatccgcaa tcatttctac ggaaacagtc tctggacact caatcactac
     9301 gcaagcagag ctgttgtggg atgtataggg gatcagttcg ttgacgagca gttgctgccg
     9361 aactactccc cagactttac atccccgggg gagctccctt tcgaaccagc gggcaagctt
     9421 tcctgtaccg cgatccgaat ctttgacatc agtatcatca aatcctacaa agattcgtat
     9481 cactctaatc gatctctgtt tcttttcttg caattacttg tacagcggtt gctttgaatg
     9541 aagctgttac agccttctct tctgacaggc caagggattc ctttgactca ttcgttatgt
     9601 atgccaccag aggaaatcca caatcaaggt acactttgat aaaaggcccc attggcataa
     9661 tacaggttat cctggctgta aaatagtttc tcaggcttgt tttcttgcta acaaaattat
     9721 tggacagaat cacattttcc ggacttatgt gaataaccac actctcgccc tcactcagag
     9781 gctcagtaac ctcgatgctt tttcccgata cagacactat ccaggtgcca ccctctccct
     9841 ttccaactac attcccggtc aaaactgttt ccacaccaac aaaagaagcg acaaactcat
     9901 ctaggggatt gttcacaagc tctgccggcg ttccaagctg ttctatcttt ccgtttctca
     9961 taaaggcaat gcgatcagag agtcggaggg cctcattccg atcatgggta gcagagattg
    10021 tcgttgtctg agactcaagg agaatctttt ccagatcacg ggttaaaccc tctcgcgtcg
    10081 gaggatcaag tgaggcaaag ggttcatcga gaaaaagaat ctctggtttt gttgcaaagg
    10141 ccctggcaag gcttgtgcgc tgtgcctctc cccctgacaa tgtccgcgca gagcggtccc
    10201 tgaggtgtgc tatgccgaac cgctccagat tctcagccac ccgttttcta atctcttttt
    10261 tgtcaacgcc tctgaatgtg agaccagacg ctacattctt gaaaacggtc gtatcaaaga
    10321 gcaaaggctc ttgaaagacc attgcaaccc gccggcgata gtcgaaaaca ccgtggtctt
    10381 tgccaataat ttttcccctg aacataacct caccctcaaa gcttttatga aggtagcata
    10441 tgttctgcaa cagagtggtc ttaccggctc cgttcggtcc aatcaggcag agaacctccc
    10501 cttcgcgaac cacgaactca ggaacgtcca gtatcgttac gccaccccgg actacccgca
    10561 aattacgggc actaagaata gcatctgtgg ccggaagctg gatgttcgaa gcaccatgct
    10621 gtgtatctga accgggaacg ctgaaggctg aagtctcgct catcgcggtc tctccctttg
    10681 ctggacatag gtaaggacaa gattaataag gtaagccaga aacaatagta tgatgctgag
    10741 ggcaattgca atatcaaaat ttcccttgct tgtctccatt actgtggctg tggtcaaaac
    10801 ccttgaatat cccttgatat taccacccac cattattgac gcaccaatct cagagatgac
    10861 accgccgaaa cctgccataa ctgccgcaag aaggggcagc tttgcctctt ttacaagcat
    10921 ccagaccatc tgggcacggc tcgcacccag ggcgagtatc tgaagcctca ttttttttgg
    10981 aagctgctgc aatgaagcaa gggtaattcc tgtaacgatg ggcgttgcaa tgatggactg
    11041 ggctatgatg attgcagtgg gggtgtagag aagttcaaga aacccaagag gaccactcct
    11101 ccaaagaaat atggaaacaa aaaggccgac cactaccgga ggaaggccca ttccagtatt
    11161 aacagcgccg acgacaatct tttttcccgg gaactgcgtc aatgcaagag cagaaccagc
    11221 agagaggcct atgaaaagac taaccagcgt tgctgaccct gaaatcttga gggagagaaa
    11281 cataatgctc atgacctcag gatcaaaagt cacgagcaac cagaatgctt ttataattcc
    11341 ttcaaatatt agatccatgt attaaatccg tgacgcgtaa cgcgtaatcg gtgatccgtg
    11401 tgatatctgt tctgtgacgc gtcggcattt ttttgaaccc gtcacacgtc actgatcacg
    11461 cgttacggca gttactattt ccctaagtct tccacttttt tgccagcatc agggaagaaa
    11521 agcggtgaac caaacttgtc gacaccaaag gtttttatca tgtcctgggt ttcttttgaa
    11581 accatgaaat ctgcaaaggc ctttccaccc ggagcattaa ctttggggaa ctttgccgga
    11641 ttcacttcga ttacgtggta gatgtttagg aggatagcat ctccctcggc aaggatatcg
    11701 aggtcaagtc ttttcttcag tgccaggtag gttccacggt cggccagtgt gtatgccttc
    11761 ttttctgaag caatgttcag ggtctgtccc ataccaaggc cggtttcatg ataccatttc
    11821 tgcccttcat atttaatggc ccctgccttc catatatcct tttcctttac atctgttccg
    11881 gacttgtcag ctcgggatat aaaaagattg cctgtagaag caatcttctt gaatacatca
    11941 acacttgact tgattccttt gattttagcc ggatcctggc cagggccaac cacgataaaa
    12001 tcattgtgca tgacaagcct tctgttaatt ccaaaaccgt ctgctacaaa ttttttctca
    12061 gctgccggcg aatgaacaag gagcacatct gcttcacctt tctgtcccat agccatggcc
    12121 tggcctgagc ccactgcgat agtctttaca aaatatcctg tctttttctc aaaaactgga
    12181 attaacacat caagaaggcc tgaatcctgg gtgctcgttg ttgttgcaag aataatattt
    12241 ttctgctgag caaaagcctc atcaactgct ggctgcgcac ccaaaaaagc gattaataca
    12301 gcagttacaa caaatactgt ctttaaaaat ccttttatct tcattaattt cctccttaat
    12361 aaatatattt gacattatgc gtatcttttt agatctcttt attccttacg gcaagcccct
    12421 cttgccgtta gttccccttc tttccctgtg attcccgcca ctctttagag ttagggaaaa
    12481 acaaagggct gccgaatttg tcttttccaa aatcccttat gatcaactgt cccttctcag
    12541 gggagataag ccatttcaca aattccatgg caggctcatg atttactttt ggaaacttgt
    12601 tgggattcac agggatcagg gttataaaat tgagaagggc ttcgtctttt tctgcaagga
    12661 tgactgtctt gatatccttc tgcaatccaa gaaaggaagc gcggtctatt acagtataag
    12721 ccttttgctg atcagtatat tttaaagttg ccacattgcc ttgagttccc ttttcatata
    12781 ctacatacca gttgccggat ggcttgatgc cggctttctc ccacaaaacc acctctgcga
    12841 catgggtgcc tgacttgtcc ccccggctga taaaggttgc ctttttttct gaaatagtct
    12901 tgagggcatc aattgctgat ttcatacctt ttattccggc cgggtcctca ggtggtccca
    12961 cgataacaaa atcattatac atcaggtcaa accgttgagt tccataacca tctttcacaa
    13021 atttctcttc aagcgatttc gcgtgaacca tgaccaggtc gatgttgcct ttttcagcaa
    13081 tctttaaagt cgcccctgtt ccggccccta catgcctcac ccgaataccg gtttccttct
    13141 caaactggtt ttccagaaca tccacaatac cagcatctat aggaccaatt gtgcttgcca
    13201 tgaatacaaa tttgtctcct gtcgttccac atccgtatag gatgggtatt accatgaaaa
    13261 gacaaattgc aaaaaaacga acctttcgta tcataacact cctccttctc tcaattagaa
    13321 ttttataatt cagataataa aacctaatgt atattatact taaaatcaag gtagaaaaac
    13381 agactgatac ccagtcttgc ccattaaaaa tcataaagat gaccattaat aacttatcat
    13441 taccaatatt aactttatca gaaatgccaa taagtatttg aaaatgtaac aatttttcgc
    13501 tatacttatg tcatgcaaat tctttctgca aaagatcttt caaagtatct caagatcaat
    13561 gagaagaaga tttacaagct cgcacaggag tcaaagctgc cgtacacaaa gatcggcgga
    13621 aaaatcgcct ttacaaaaga actgattgac aaatggattt tggaaaacac ctccagagaa
    13681 cacaacatat accttgcagg cagcgatgac attctgctga gaagaatcat agacctctat
    13741 aaccagcgca gagatgggac tgtattctac gcaccaatcg gaagcatcaa tggcttgcgc
    13801 cttctcaagg attctgccgc gactatgtct tgcgttcaca tccttgacgt ggaaaagaaa
    13861 aatcaggacc tcgcatatct cgacaggtat ctgtcaaagg atgattatat cgttgtagag
    13921 ctttacaccc gagagcaggg gcttatacta accaaaggga acccaaaggg tattcggggc
    13981 atagaagaca tagcctcaaa aaatgtcaaa ttcaccaacc ggaatcaggg ctcgggaaca
    14041 aggattctgg tagatttttt gctccatgag aaaaacattg atccgggaaa gatacagggc
    14101 tatgcattgg aggcagattc ccatctcgag gcaggactga aggttctcca gggcgaagct
    14161 gaagccacat ttggaattcg tcatgttgct catatgctta accttgattt cattcatcaa
    14221 ttcagagaaa gattcgatct tgtcatacct gttgatcaat actattatgg ccacgtcaaa
    14281 tcatttctcg ctttttttga acaaccgacc cttcttcaca atatcaggga ttttaccggg
    14341 tatacgatca cgaagatagg gagcgttatc cataagaatg tatgaacata tcaggcggaa
    14401 tatctataat ctctttgcaa acagaggcgt acgggtagaa gtcgacgaaa ctgttgactt
    14461 tcccaatttc tgctgtctta agattcaata tcaaccagaa caaaacctca ccctcattat
    14521 tggaaagttc acagatgatg tcctcgaaat acttcttatc gcccatgaat tcggccatat
    14581 catgcattat gaaaacctgt ccagagaaga tgcagaggct gcatactgcg caatttttgc
    14641 atcaaatcat ctgggactgg aaaatatttc tcctgaaagc aagcaactcg ttgtaggtat
    14701 tgaaaagagg gcctctgagt atgctatctc cctcttaagc gttctcggaa gtgatgaaac
    14761 gatcctctca agggcaaaag aaacgtacga tgagtggatc aggggctacc tcaaaaaaac
    14821 cggattaagc gaaagagtta ccattctcgc gtcttctcat gagtaaaacc gatttacaga
    14881 acattatacg cgtggatatc cttgtcaaaa aaggtcaaga agaactattg actgaccaaa
    14941 tttaccagtt tatcaacaaa ggggtttggc tggaagagca aggtaatgct gtcctgatca
    15001 aatgttatcc tgaagaacct gaatctttta tacagcaagt acgagtgtct ggccttaaaa
    15061 ttacgggcat aacaactact caggaaaaac tccaagatta cagtgaactc accagacggt
    15121 atttcaggcc aataacaata ggaaatttta caattcgagc accctggaat aaaaagaagc
    15181 ctatcaatcg ccaaattgta attgaacctg gcatggcctt tggaacaggc agacacgaat
    15241 ctacaaaaat catgataaaa atcatggaca gcgtggaatt ctcaggaaaa aaggtccttg
    15301 atattggctg cggatcaggc attctttccc tgaatgccca cctcctcgga gcgagaagaa
    15361 tttacgcagt tgacaatgac ctagacgcga ccatcaacgc taaaaagaat gtattcctga
    15421 accaagccaa cactataaat ctagtatgcg ccaatctcga ggacattaag ggaacttatg
    15481 acattgtgct tgcaaatatc gatataaaaa catttgcaga acattcagaa cggattgtag
    15541 ccctggtgaa agaggatggt cttctcatta tatcaggaat cttacgaaaa aatagagacc
    15601 aactcctcca tctctttcat gattggaagc ttctcaaatc ccatcgtata cattcctgga
    15661 tgggattcct ttttagtcga taacattcca ttcatttcga ggctttttag aaaattccct
    15721 tgctttttat ctaaactctg ttatactaca caatagaacc taataataac caataataca
    15781 ttattcttaa cagaaaagtg tgatgtgata attttgatta tgttttacct gctcatatag
    15841 cccaatctga gaggagcagt gctcgatgtg ccgtcgattt gctgttcctt cctctgccct
    15901 ttcccccatc cgttcccgga tgggggaatc ttatttttgg ggctccagcc gccccacgtt
    15961 tatcttaagt caaccttttt atcctcatgc atcaaggata ttttctgagg ttgcccaact
    16021 ttgctgcttc atagtcaatt tttcttccat ccctcttcct ccatcttcaa tcaggcttca
    16081 agtctgagct tgcgtctata cacacagagt agcccacttt ttcaacaaac aacagattat
    16141 ttgactggtt gattgaagtc tttacttgtt attcctcaat tatcaatttt acttccttgt
    16201 ccgagctttc caatttattg acaagcatat acgcatcggg taatatacac ccatgattga
    16261 acgatacacg ctaaaacgca tggcagatgt gtggtcagat gagaatagat tcagaaaatg
    16321 gcttgaaatc gaagtgctca tatgcgaagc ctacggtagc ttatcagaaa taccccctga
    16381 agaccttaag tccattaagg agaaggctaa tttcaacatt gatagaatca atgagattga
    16441 gaagcgaaca aaacatgatg ttgttgcctt tattgaatct gtggcggagt ttgttggacc
    16501 ttcttcaaag tatatacaca tgggggcaac atcatccgat atccttgata catcctttgc
    16561 ctgtctcctc aaagaagcat cagaaatatt gattgctgat attgagaatt ttatgacagt
    16621 tctcaaggag aaggcctttg agcacaagct cacgcccatg attggccgca cccatggcat
    16681 tcatgcagag cccatcacct ttggtttaaa aatggcccac ttctacgatg agatgaggag
    16741 aaatcttgaa cgcatgaagg ctgccaaaga gagaatcagc catggaaaga tatcaggagc
    16801 tgttggcacg tttgcccatc ttcaaccttc tatcgaagaa tatgtctgca aaaatttggg
    16861 gctgaagcca gcgcccatct caacgcagat agtccctcgg gattatcatg cagagttttt
    16921 caccacactg gcaatcgtcg cttcgtctgt tgagaagatg tctctcgagg ttcgaaatct
    16981 tcagagaaca gaggtgggag aagtcgagga gttttttgct aagggccaga caggttcatc
    17041 cgcaatgccc cataaaagaa acccagtcgc ttcagaaaat ctctgcggcc ttgcacggct
    17101 tcttcggggg tatgcactat ctgcccttga gaatatcccc ttgtggcatg agagagatat
    17161 cagccattca tccgtagaga gggtcatcgc tcctgacgca accatactcc tcgattatat
    17221 gcttgaacgc gtaaaaaaca tgtataaaaa ccttatggtc tacccggaca ggatggaaaa
    17281 aaacctgtct atgtcacagg gtcttcatca ctcagaatcc atccttctga gcttgatcaa
    17341 aaaggggctg acacggcagg aagcctataa acttacgcag tccgtggcca tgaaatgtta
    17401 cgatcatgga ctggacttca ggactgaact gaagagggat caaagtattg cccaattctt
    17461 acaggaaagt gaaattgatg acatcacaac aaacgagcat tacttcaagt atgtagatac
    17521 gatcttcgca cgggtattca taaaaaaacc cctgtaatat taaattgtca gcatatttct
    17581 atctttattc ttcattctcc atccacttaa ttagtcaagt ttatcccttc ctatcactcc
    17641 ttcccgaacc agtctgttga tgcaggacgt tctccaccaa ctacctttcg atctccgacc
    17701 cacctggctg cttcctccgg agtcccaaag taatgatatt tggcaaacac cccctgacct
    17761 tcagctgaca gcagaaatct ctggaagtct tttgcctgct ccatcatctt tgagtgggag
    17821 agaattccta tctgcccggc accgatacgc tgtatctctt caggtcgcag ctttattgtc
    17881 tcaatagcat ctggatacca tgagctaaga taatgaaagc caattatggc atctactttg
    17941 tccaaaagga gcgcgcttgc gagtttgttg aaatcctcag gcgtggtgac tatgttcttc
    18001 tttaaaagtt ttatatcctc acgggtcagg cttttctcta ctatttcagc cgcgagcatc
    18061 cctacaaaca ctatctccgg attggcaatt gcaacccgca ctgacggctt agcgaggtct
    18121 gctaaactcc ggatcccctt tagattaccc ttacgcacat taataacagg cacaacgtaa
    18181 gcaaaatcct ttcttgaatc aggcacaaca aggcctttag actgggcctt ctccataatg
    18241 tatgatgatg gcaccattaa tatatcacct cttttactca tcgttatctg ggacagcagc
    18301 gtgccgaccc cgccgtatat caccctcaca tcaatgttgt tttttttcat gtacctctgt
    18361 attatctcat ccataggctt cttgtaggct gcgccacaaa aaatcatcaa ttcatctcgt
    18421 gcaaatacat ttccaaaaca cataacaacg aataaaagtg ctactaacgg ccacaggaat
    18481 cgtgttttca tacttctcct ccatgaatca atctgtatgt ctatggttca catgatcgtt
    18541 atatacaata taatataacg gcctgtcaag ggttttttta caagcaatta aagcaataat
    18601 cttgtgtcaa gtatgttttt atcctgaata cattcttaaa tatccacgga ttaacataat
    18661 tcatcccgcc cataactact gtttcatctt accttcgtgc tactccttga gcaaaatgtc
    18721 ctatgtctgt tttttaccgg cagatctttg aatctgtttc atttgtagtt atggcatgta
    18781 tcaagatctt gctgcaccat ttttggctgc ataacagctt atttatcgat ttgacaatta
    18841 tttctttccc atttgccata agttgcaaac ttgtagtctc aggaattgac aattactttt
    18901 tctgcaacta tcctttaata ttatggcata aggaggtgct ggactatgaa gctaagtgca
    18961 cggaacatct tgaagggaag agttaaaagt gtcataccgg gtgtagtcaa cacggaggtt
    19021 aaagtagaaa tcgcaggggg tgccgaggtt gtttcagtta ttaccaaaac atcagccaat
    19081 agtttaggtc ttgtggaagg caaagatgtg tatgtagtaa taaaagcaac aagtgttatg
    19141 ctcgcagtag attgatcttt gtttcgccaa ttgccggaat agggtttggg caccatttta
    19201 ttccagccat gagctaacta tcgtcaggct tcgccttttt cgttaaattg ataactatca
    19261 ggctgaaaaa tatgaattac ctctggctca aaagacagaa aaacttcagc tccgtcaaaa
    19321 aacttactct ccaaaaggat tttcttggaa acgactgatt tgaatgtaag cttttgcact
    19381 tgaacatgca cctcgtaata aaatccttgg tcaactactg cagcaatgat tccgctaaaa
    19441 gcatttatat tgtttttgtc atattttttt ttggtaataa ctatatcatc aggtcgaatt
    19501 gcaatccgtc ccacccttac aggggagcga cagcctatat ttaactccaa accatcaccg
    19561 atgattgcct ttgatccatt gaactgtgcg tcatatatat ttttcattcc tacaaagtca
    19621 gcaacaaaca tggttctcgg tctttgaaaa atatcttcca ttttccccac ctgctcgatt
    19681 cttcctttgt tgaccacggc acctctgtta ccaagcaaca gtgcttcggc aaaatcatgt
    19741 gtaaccatca aaaatgtagt acgagaggat tggtgaatcc tctttaactc gtgtcggata
    19801 tcctcgcgga agttcgggtc aagggctgac agaggctcat caagcaaaag tacgcttggg
    19861 tttaccatca aggctctcgc cagagccaca cgctgtaatt cccctccgct tagattgagc
    19921 ggtagacggt ttacgaggct cttaagctca aattcctcaa tcaactcctc aaacctcttc
    19981 tctgtttcct tatgttcata tttgtgaaaa tgaaggccat acctgatatt ttctgtcacg
    20041 gtcatgtgag gaaacaaaac atagtcctga tagactatgc ctactccgcg cttttcaggt
    20101 ggtagtcttg taacgtcttt acctgcaata aaaattttcc catgcctcac cgggatcaaa
    20161 ccagcaagag cctccaaaag aacagtcttg ccggcaccgg ttggtcccat aagaataaaa
    20221 aactcccctt cttctattga aaaattgatg tcatagatgt tgaaaccgct cagatgggtt
    20281 tcgagactct caatctttat catgttttct tcttgtttgg tagggtcaga taacgcagga
    20341 caaaaaataa actgagacaa accattacca accaaacagc aactggctga gaatacttca
    20401 gaccataccc ttcaaaccgt tcataaataa gaacaggcgc aatcatcgga tggtaagcta
    20461 caacaaccac cgccccaaac tcactaattg ctcgggcagc acacatgata atacctacaa
    20521 ccatgctacg ccacgccaga ggaaaggtaa tccgaaaaaa ggtgctgaac atagacgcac
    20581 cgaggcttcg tgaaaccctt tccaatctgg gagatatatt ttcaaaacct tcttttgctg
    20641 catggacata gaaaggcaaa cccacaaaga ttaagactgt aacaatcccc gtaacgctcc
    20701 ccataactct cactccaagc tcagcaagac ctcttccaat ccagtgattt ttccctacca
    20761 ctccaaggat agcgatccct acgacaggat ggggaatcac aatgggaaga tcgataattc
    20821 cctcaaccag cctttttcct ctaaagtctc tccgagcaag cacatacgcc agaggtgttc
    20881 ctacaacgaa agaaatgagc gcagccaatc cggctgtgta aacactaagc cagattgacc
    20941 gcactacatc tttgtcccgg atcgccatca ccaacatgga tatggaggga gcagtcacca
    21001 tctctatgag aggaagtaaa ataaatacca tgactataag gccacatgaa accgtgaccc
    21061 aaaagaaaag ctcagtccgt ttcattgctc ttccccacta tcgtgcaacc gggcaacatc
    21121 agaaacctgc tatgattccc ccataactat tattttttct ggaggaattc gtacttttac
    21181 ttcgtctcct tcattaaatt ccctatcgtc catagtatac ataacaacag aaataccgat
    21241 atctacacac acatcttgaa aatacggttt taatacactt cccctaactt tcccttgaaa
    21301 aatgttactt tctttaccat catcagggcg aacaattgta atattctctg gtctaactgc
    21361 aacaacaaca tttcctatgc aatcattttt cttcaccagg acaatgttct ctatatttaa
    21421 aacagagcca tctgagacac ctttaaaaat attctgtgat ttcaagaatc ttgccacaaa
    21481 ctcgtttttc ggatactcca tgatttcttt taacgtacct acctgaataa ttctgccatt
    21541 atccatgatt gcaactcgat ctgcaacatc agcagcctct tcaaaattgt ggcatacatg
    21601 aataaaagtc ccatttgaat ccctttgtat ttgtctcaac tctcgtgcca tttcggtcct
    21661 taggttttca tctaaggcgc tcaatggttc gtccaggagg agcactttag gctttgttgc
    21721 caaagctctt ccaagggcaa ccctttgttt ttcaccgcca ctcaaatgaa gaggcatcct
    21781 gtgcttgata taatcgattt caagcctcgt gatcatttca gaaacaatgc tgttcagttg
    21841 ctccggttgc attcttcttg cctccaagcc ataggagata tttttttcaa ctgtcaaatt
    21901 tggaaaaagc gcatagtctt ggggtacata accgatgttc cggtcctcca taaaaagatt
    21961 agttatatct tcaccatcaa cctttatact tcctttattc gggttatgaa tccctgcaat
    22021 gtattctatc agtaccgtct tccctgcccc tgtcggtcca agtaatgcaa gatattcgcc
    22081 ttcgttcact gtaagattga tatcacggag gcaaaattct ccgagatcac catttatgtc
    22141 tgtgagttca atcatatctt cccccttacc atacataccc ttttgcaccg atacgatgca
    22201 taacataaac cgaaattcct ccaatcacta caagtacata ggcaagcgca aaaccgtact
    22261 cgacatttcc gaactctact tgcgcccaca ctgcaactga tacccaatct gcctgatcca
    22321 ttttaataag tttcgtaaaa ttggaaaatc gtgacggagg caactctcga aatgtaccac
    22381 agaaaaacaa aactgcactg aattcagcca tagctcttgt ccaagcaaga ataatcccgg
    22441 caataatgcc gcttttagcc aaaggcaagg ttacagtcct gaatgctctc catctgctgc
    22501 tccctagtga acgggcaaca tgctcgaaac ggggattaac actatcaaaa gcagctttcc
    22561 aagcacttag ccccaaagca aaggaaagta caagggaggc gagaaccatt cctggaaaaa
    22621 gactgtgggc aaacttaaat ccaactgcat tctgcagatt ccataatgga ccataattga
    22681 acattacaat caaacaaata ccaatcacag aaccaggcat aacgataagc gaagaaaaga
    22741 ggacttcgat agccgaaata cctggaatac gatatcgtga aagtacgtaa gcagtaggaa
    22801 tccctaaaac agaagcaatc aaagtagtaa tgagtgtgct aactactgtg agattgagtg
    22861 atttccaaaa gtaaggatca ttccagaacc ctccagcgaa tttatttagg acctctctat
    22921 cttcacccca gacggccgtt atcccacctg tttgaaccgc tgtctcagtg acaaaaagct
    22981 gatccacatt gctaagaaaa agatacgccg aatagaggaa caaaaatccg gcaaaactgt
    23041 aaaaaagcat attaaaactc ttcctgcgcg gtgtcttacc agacattact tcttcctccg
    23101 atcattttca tggcagatat tgcagatatt gccatcagta tacatacaat agacattgag
    23161 gtaacccacc ccatcatccc agcattaaaa tccaagtata tggcaaaagg cataatatcg
    23221 gtcttaccct cggttgctcc aacaaacaac atcaatcctt cccactcggc agcagccctt
    23281 gcccagacca ctatcatgct ggcaataatt ccatttttcg ccataggaag aactaccctg
    23341 aaaaatgttc ttaaaggtga cgcacccaaa cttcgcgaaa ccgcttcaaa cctggtaggt
    23401 atcatatcga aacttgcctt cattaaccta gcgcaaaatg cagcggtcac tgtaaactgt
    23461 acaaggagta caccgtatac atttcttccg atataaattc cataatgttc gctaatcaaa
    23521 tcgaagggaa atctagtcgt aatcccaaag agaaacgctc caaccactgc tggagaaacc
    23581 actataggaa ggtctatgat gctggcgatc aagttgcgat agggaaaggt aaaacgcgag
    23641 agtgcataac cggcaggaat accaatacat aaggagaaaa atgttgatgc caatgacgtc
    23701 catactgtaa gccaaaatcg ttcccacatt cttgattttg aaaaaaaata aacagtgtcg
    23761 gaccaaccca agtaaatcca gttactcaat accatcagaa catatgcaaa aagaatcgtg
    23821 atagttatcc ctacagaaag tgtgggccac cacccgccgc gcgccttggc tgccaattct
    23881 attctcccct atatcaacaa tcaatcacca gcccgcgtcg tttttctctg tctcataacc
    23941 tcatctatca aatcaccaac agtttctgtg ttgacaggga aggattcgtc cttaaccgcc
    24001 ttggcagcat ttacaagata ccccatatct ttgtcggtcg tgccatccaa acaaaaacca
    24061 ttcttgtcaa caggtataac cttgttaacc gtataagcat gcttgtggaa aatgtgcctt
    24121 ccctcatcgg atagtataaa attcatcaat tcacgcgcct catttttata acgagcaaac
    24181 ttgagtatac caaccggcag tcggactatt ccacgatact tatattcaat ggggataatt
    24241 tcaacatccc gcagatacag gactgctgta gtgccccaaa cgatagtggc atcaatagcc
    24301 ttgtgctgtg ttgcattacc cagttctgga atacattttg cgctcaaagt tgcatttgcc
    24361 ttaaccttat cccagattcc cgcttttttg aacgtctttt catgccatat tccaatggca
    24421 caagattttg gatttcccaa acctacttta acgccaggtt tggcaaaatc atctatactt
    24481 ttgatgtttt tcggatttcc cttgggggtt ataatgaccg ttacaaaata tgccgggata
    24541 tcggttttgg gattgtagtc ggttacgaac cctctttcca cagcctgcat aagataaaac
    24601 tcttcaccgg gcatgaaaag atctccctgc tttgatcggg taatatcggc tataaaatag
    24661 ccggatcctt tataggaaaa atttactttt atctcgggat gtttcttttc aaatacgttc
    24721 tttatctcgt tgagggccga acgctgaccc aggcccgcgt ggaaaagcaa agccttttct
    24781 ccgccattta cttgtggaac caagaaacca gaaaataaag ccaaaaaaac aaatattctt
    24841 aaaattcttt tcacttaagc ctccaatgca tgttgtaatc ttagaagcaa aatattcttg
    24901 atcctaaaca ttgaccaaaa aagttgaaaa gactatttta attactgaac ataattatca
    24961 gtcttttact tctacaagtc cttgtaattc cactggaacc aacgccttca ttttgtccgt
    25021 cgaaacccga catggcacaa aaggtggctg tccctgttct ttcagtatct ttaaacctcc
    25081 ctgagggtcg agcatatact tcaggaaggc tattgccgct tctttgttgg gggcgtcttt
    25141 gatcattgtg atcccaaatg tcactgaatc tcctttcata tccataaatg aacccggctc
    25201 tttacctgtt accctaacaa ctgcctgctg ataaaaatta tcatacttat gatttcccag
    25261 attgatctca tctggaagaa tgacatattt cagcccatgc tgaacagcca cagagagata
    25321 ttcccaggcg tagtccatat tcccggtctg taagagagaa acaagctcta cagattttgg
    25381 tctcacattc tctttgggac ggtttgcaat cattttgtca tagagacctg gcatcttgta
    25441 atatttttct gcaagctgca aaaccataag agaacgatag ccacaggggt ccaggttcgg
    25501 atcggaatgt ccccagacta cccccttctt ctgtaatatc tcgtaccagt tatttgcgtt
    25561 tacttccttc gcgaatttgc ttttatccgt gtagcacaac acaagctggt tggttgcaaa
    25621 acggatgttc caatctgcac tgcccggaat aagcagtttg tctattactt tgtaatctgc
    25681 tgatgccatg atgtcacaag gtttttttag gtcggtaatt ttccgtgcac aggcctggct
    25741 tcctgcggct tctctctgaa gatcaacctt tggatatttt gcttcaaacg ctttttccat
    25801 ggcttcaaag ggtaccgaga ggcttcccgc gtgaaacatt acgagcttgc cttgaggttc
    25861 ggcaaaaacc tgaaccgaaa ccaacaacat taccgcaaaa catacaataa ccgtcgcttt
    25921 tttcatcttg tttctccttt catttgttgt cattaatctc actttttata atcaatttaa
    25981 ccatatccta cctaattatg catatatatt cataaaagta tataattttc ttaattgtgt
    26041 caatagaaaa actcgtttgt gttttattac acatcaccgg agagaaagaa attgaatcat
    26101 tgcagactca taatctgtta ttggctacaa ttacccattg gaaaatgtaa aaaggattca
    26161 gtggaaataa ccataaacag actggatcaa aatgcacccc agtgtggctc gaaggtaagt
    26221 tgtaaggcac acatcccacg catcgttaag ctcttttata tacctatcct aacaatgaaa
    26281 aagctgtcct cgtgtatctt tggccagtat caccagggga tgttcataag gttgagcatt
    26341 ttctgggcta ttttgttgcc tagacgggca gcaaatctaa tatcctggat ggcagattta
    26401 tagtcatcaa tgaaaaccct gcaagcccca cggtagcagt aggcatcatc aaacagatgc
    26461 tcccactcga tagcaatagt gaagtcatct attgcttctt tatagtgagc aagttgcgag
    26521 tgaagcaccc ctctaaaaaa gtatactttt tccagggatg aatcgagtga cagtgccctc
    26581 tggtaactgt ccatggcctc tgtccacata ccaaggataa aaaaagcaac gcccatgtat
    26641 ccatacactt ctccctgttg agggtttcta tctgcagcga tagaaagata ctgaagggca
    26701 ttctcgcagt ctccatcccg taagagcata accccctttt tcaaataaaa gtcattttct
    26761 gcgttgcttg tccagagcct gtcactcata tcatggggca tattattttg aaggtttccc
    26821 gttttttgag acaagccatt ctataatggg tcgatccacc tctgggaatt cataatcgat
    26881 aaggtcttca ggctttaccc atcgtaaatc ctcatgatcg tgaagattga gagaccccga
    26941 cactatagcg gccttatatg ccattagctt aactgtccag tcagatgcgt acctgtactc
    27001 gtttgagcaa atcagatcac cgatttcaga tgttacggtg agctcttcct tcagctcccg
    27061 cttcaggcat tccttatacg tttctccctc ttcgagtgtt ccacccggga actcccagtt
    27121 gccggcgcgc atctttcccc tttttctttt ggctataagc acatgaccat ttctttcgat
    27181 aacagctact gttacgactt gcgggtttac ctttttcttc ttcatctgat caaccaaact
    27241 cgtcacctat atatagatcc tggtggaacg tgatttcaca cactaattta gggttcgcaa
    27301 tctctataaa tttatggttc ggttattttt aaaaattacc ataatactgg ttatataaaa
    27361 agataaattg tgtctataac aactaatcga tattatttca tactatttat tactaattaa
    27421 tcttgaaatt atatttctga agcgataagc taatgaataa tgattaatta tactgataaa
    27481 taagaataat acctgattct gcataatttt cgtgcggaaa caaaggagga gcgtggatga
    27541 agattacctt ggatggaacc gtttatgaag ctgaacccgg tcagacaatc ttggaagtag
    27601 caagagcaaa tgggataaac atcccaactc tgtgctatat gccagggctg cctccttttg
    27661 gagcatgcag aatatgcctg gtggaggttg aaaggtcgcc agctctggtc gctgcctgca
    27721 cagctcctgt aatggagggg atgaacataa agaccaacag taaacgcgtg atagatgccc
    27781 gtcggtttgt tgtgcagctt cttgtctcat gtcaccccct tgattgtatg acatgtgaaa
    27841 agacagggga ttgcacatta caggacctgg cctacgagtt gggagtgacg aatccgcgcg
    27901 aggatgaagg agatcgacac aagtatccca tagactcctc caatcccttt attatcaggg
    27961 attacaataa gtgtatttta tgtcagagat gtgttcgggt ttgtgatgaa attcagggga
    28021 tcgttgccat caaaaacatc aatcgcggtt ttgacacaaa aatagccacg gcatatgacg
    28081 gcaccttgca ggacagcaac tgtgtttttt gcgggcagtg cgtgcaggca tgccctgtag
    28141 gagctctggt gccgaaaaaa gccgtgggca agggacgtca ttgggagtta caaaaagtac
    28201 ggactacctg tccctattgt ggagttggct gtcagcttaa cctgcacatg aaaggagatc
    28261 agattgccta tgtctcagcc gttgaagatg ggtcacctaa caaaggacgt ctttgtgtaa
    28321 aagggaggtt tggttacgat tttatctatt ccgaggatag gttaaagaca ccgcttatca
    28381 aagaaaatgg cgcctttcgt gaagcttcct gggacgaagc cctggacctc gtcgcatcaa
    28441 aattcaaaca gatcatctca gaaagtggtc ctgatgcaat tgcaggcgtc agttgcgcac
    28501 gcagcattaa tgaagattca taccagatgc aaaaactctt tcgagcagtg atcggcacta
    28561 acaatattga tcactgcgcc cgtacctgac acgcccccac tgtcgccggt ctggcgcaat
    28621 catttggttc tggagctatg acaaattcat tcggagagtt tgctgatgca aaaatgttcc
    28681 tctgtattgg aacaaacatg acagaagccc atcccgtggc tgccacattt ttaaaaaatg
    28741 cgctccggaa aggtgcaaaa cttattgtgg tggatcctcg aaaacacgaa ctcgcaggcc
    28801 ttgctgatat tcactgccag attaaagttg gcactgatat cgcctttttg aatggcctca
    28861 tgaacgtcct gatcactgaa gagttgtacg ataaggattt tgtacagaac aactgcgaca
    28921 attttgaagt cttcaggaca aaaatacttg aataccctct agagcgggcc tctgctatct
    28981 ctggtgttga tgccgagaca attcgacagg cagcccatat gcttgctgaa ataaagcctg
    29041 ccatgctctg ctacactctc ggcattacag aacatacaac tggaacggac aatgttttaa
    29101 gcacagcgaa ccttcagtta ctcttgggaa atatgggcgt cccctcagga ggagtaaatc
    29161 ctctgcgagg ccaaaataac gttcagggcg cctgtgatat gggggcacta cccaatgtgt
    29221 tccctggata ccagaaagta atcgatgaat ctgtaagagc aaagtttgcc aaggcatggg
    29281 gggttaagaa tctgcctgat cagaatggcc tgatgatgtc ccagatgatg gaggggctgg
    29341 tcacgaaaaa gattcgcgcc ttttatattt ttggagaaaa ccttgcaaac actgagcctg
    29401 acattcataa aaccgaacat gaacttgctt ctgcagaatt cctcgtgtgt caggatattt
    29461 tcccaaccga gaccacccgc ttcgcccatg tagtctttcc tgctgccgca tggagcgagg
    29521 atgatggaac atttagctgc agtgaacggc gcataagccg ggtacgcaag gttaaaaacc
    29581 cgcctggcca agccaaaccc aactggtgga tcttcaaaga gcttgccagg cgcatgggcc
    29641 aggaatggca atccaatagc tcacaggata tatgggacaa tgaagtctca gttctggctc
    29701 ctgcattcgg tggtataaaa tacaatcgaa tagaaaaaga tggccttcag tggccttgcc
    29761 ccaacataga tcacccgggc acacagttcc tgcacaaata tggggtgttc acccacggca
    29821 aaggcatatt cagtcctgtt gattggaagc cgccagcaga agtaccagac gaagagtacc
    29881 cttttgtcct gagtacaggg cgccgccttt tccattatca tacgcggacc caaaccggcc
    29941 gaacagggct cgattttctt ctgtcagagg aaacagcaga tatttctctg gctgatgcac
    30001 agaaacttgg tattcaagat ggggaataca taaaagtccg atcccggaga ggtaacgtga
    30061 cagttaaagc aagggtgact ccagaggtac cccccggcat ggtctggatg gcctttcatt
    30121 tcagagatgc atgtgccaac tggctgacca atgccgtgta tgatcctgtg acatttacag
    30181 cagaatacaa ggcctgtgca gtagctatag aaagataaaa aaacatgcga cagttggtga
    30241 gtacagaaaa ataaacatcc tgcccccgtt ggaggaatat gtgatatctg tgtgtattgc
    30301 ccggactcac ggtgcccata tcaggggttt tctgtcaggt gtaggatgtt tactccccct
    30361 tcgttttaag atctgacagg aacatacatt ttaaccaata tcatcccctc gcttgaatta
    30421 tcaggattca acacaggata tttcaaacca gtcattctga ttcgcattat agtgtttatc
    30481 tatgtttttt gtatttatta tatttatatt gttcatattt gttttgatat ttcttttttt
    30541 cttgacattg ttcggataac tcaggtatag tcagtcatta gagcctggtg attttaggac
    30601 cctgggatat tacgatagct cgtatgttgg ggggtgatgg agcctccttt gcctgatgag
    30661 aggcaaagga ggtttttatt tgccctatga cgaacatgga aacaatttag cctgcaaaaa
    30721 gatgacggca ctatgaatgc tattctttgt aagattcaag aaccttgaat ctacaaacat
    30781 aagaaaggag gggcactgat gaaaaaagta gtaatgaaaa tcaatggaaa attgcaccaa
    30841 tttgtcgttg aacccgatcg tgtcttgctc gatttattga gagaagacat gcttctgacc
    30901 ggtgcgaaac agtcatgcga tcgcaagggg cagtgtggtg cctgtactgt tattgtgaat
    30961 ggtcaagctg ttcgctcctg tcttcaaaag gttgcaaacc tcgatggcgc agaagtgata
    31021 actattgaag gtctgggtac accagaaaat ccccatctga ttcaagaggc atacgtcctt
    31081 tcaggcgctg tacagtgtgg attctgtaca ccggggatga tcatggcaac aaaggccctg
    31141 cttgatcgca atccaaatcc caatgtcgag gagataaaaa aggctcttga gcacaatctt
    31201 tgccgctgca ccggttacat caaaataatc gaggcagttc aattagctgc tcgctttatc
    31261 cgcggggaga ccacacccgg ggcggtcagg cctgatccta atggtccaaa gattggtgtt
    31321 tcccatccac gaccttcggc catgctaaaa gcatgtggtg tggcaccttt ttctgcggac
    31381 atcaagcttc aggatgcttt ggagcttgca gtggtgagaa gcaccgaaca ccatgcgaac
    31441 attgtttcca tcgatacatc aaaggctgag aagatgccag gtgttgtcgg cgtcataact
    31501 gcgaaccaca tcaagggcac caaccgaatt gtaatcatct tccctgacga gccaatactc
    31561 tgcaaagaca aggtccaatg tctgggagat cccattgcga ttgttgctgc ccagaccaaa
    31621 cagcaggcca ttgccgctgc agaggctgtc aaagtaacct atgaaccgct gccggttctg
    31681 aggagtcccc aggaagctat ggctgaaggc gccatccaga ttcacaagga gtggcccaac
    31741 ctttgctttg tccagcccca gataaagggt gatgctgaaa aggccattgc agaatcggct
    31801 gctgtggtcg aagccagctt taccactcag ataaaccatc aggcaccgct ggaaccggag
    31861 gccacagtcg catacatgga gggtgagggg gaagatgccc agttggtggt cgtgggacga
    31921 agcattcaaa tccacggcgt tatgaccaac ctgcaggaag cgctcggcta tgagaacatc
    31981 cgatacgagg aagcatacag cggcgggcag ttcggtataa aagcagcaat tacgactgaa
    32041 ggtatttccg ccgcagcagc acttcacctt caacgcccgg tgcgatatat accaagcatt
    32101 gcggaatcca tgcttctcac caataaacgc cacccctttg acatgaaggt taaacttgct
    32161 gcagacgaaa acgggaagtt gaccgccttc accatcgatt acattgttga caacggagct
    32221 taccagatta tcggaatata cgtcataatg cgtgctatgt ggatgctctc cggctcgtat
    32281 aacatcccga atgtcaatgc aatgggcaaa ctcgtctata cgaacaatcc tgccggaggt
    32341 gcagcacggg gcgctggtcc acctcaggtc acttttgccc ttgagtcagc catggatatg
    32401 ctggctgaaa agatcggcat ggaccctctg agattccgaa agatcaattc cctcctgcca
    32461 ggccagagtg tatcgaccgg catggtttac gaacagtggc ctttcccaga gctttgtgat
    32521 gcaatccagg caaagtatga tagtgcaaaa aaagaagcgg ctacccttaa aagcggtccc
    32581 attaaacggg gggtcggcat agccacccac gccttcggca tagggggtcc tggtgatgca
    32641 ggcagggttg ctgttgagct ggacccagat gatggtatca ccatttatgc cgcagtcgct
    32701 gacccgggtg aaggcaatga ttccatgtta acacagattg cctctcattt aaccggcata
    32761 cccatggaca aggtccgtct cgtgaccaga gatacagacc ataccgctga aatgggtcca
    32821 gccgctgcca gcaggatgac atatatggca ggcgggtccc tggtcctcgc cattgaacaa
    32881 ctcaaaaaag ctatggaaga ggccggtgtc aaaacatatg aaggcctcac aaaggcaggc
    32941 aagcctattc attacgttgg atccaaaaag gctgcgggac aggaaggtcc gttggatcct
    33001 gtaaccggtc agggcccctc tttcgaatcg cgtgttcata acattcagat ggccgaagtt
    33061 gacgtcaata ccgatactgg tgaggtgcag gtaataaaga tgaccacagc agttgatgcc
    33121 ggtacagtca tcaaccccca gaacctcgaa ggccagttgc agggaggtat ggatcagggt
    33181 gtgggctacg ctctcagaga agagtatgtc catggcaaaa ccagggactg ggtaacattc
    33241 aagttcccta ccatgaaaac atcctttcca acagatatca ttatcaggga aaccccacgg
    33301 gtaaagggtc cgctgggcgc aaccgggatc ggggaaatga ccatggtatc gacggcaccg
    33361 gccatcataa atgcaatcta tgatgcgtgc ggagtaagaa ttttcgattt acctgctacg
    33421 ccagaaaaaa taaaggcagc cctggctgct aaaaaataac cggcaactgt agaggttcag
    33481 gatgtcccga tttcatgttc cttgagaagg cgggacatcc tgggttatcc cgaagggtac
    33541 aatgaatgac tctgcaaccc atacccttga agcaggtgat gtccaccaga aattccgaac
    33601 tattgcaggc agggaaatac tttttgacaa acaagggttc ctgtggcatc cggaagattg
    33661 gtctgaagag gtggctgtgg cacttgcatt agaatccggt attgaagcat tgagtaatga
    33721 ccagtggcgt gtcattcgtt ttctccggga ttattttgct tatcatggaa gagcgccttt
    33781 gaatcgcgat ctcaaagccg gtttaaatat aagcctcatg aatctggagt ctctttttcc
    33841 cggggggatt cgtcatggcg cccgacgcat tgccgggtta ccgaatccga gggcctgcac
    33901 aggataggaa gtatgctcca aagattaatt tacctcgata atgctgcaac aacatttcca
    33961 aaaccagcat ctgttttaaa ccggatggtt gaaatttatc ttcaaatggg cgtatctccg
    34021 ggtcgaggaa gctatgatat ggctgttgag gcagaggaaa tggttaccaa tacacggcag
    34081 aagctggccc attttttcgg ggcccctgat ccggaccggg tcatttttac aaacaacgct
    34141 accgatgctt tgaatttact cctgcaaggg atgctgaaac ccggggatca tgttgtatcg
    34201 accaggctgg agcacaactc tgttctgagg cctctcaacc acctgcacat gaagggcatc
    34261 atcgaatatg atctggtccc cttcgatgca aatggatttg ttgaccctgg cgatatcctc
    34321 cgttcaatta gaactaacac gaaactcgtt atcatgtgcc acgcatccaa tgtcctcggg
    34381 acaatccaac ctgttcagga ggtaggacgg ttgtgctcag agcggggggt gcctttattc
    34441 atcgacgccg cgcaaacagc aggcctgata ccaataaata tgaaatcatg gtttcttgcc
    34501 ggggtagcct tcacagggca caagtctatg ctcggtccaa ccggtatagg cgggctggtt
    34561 ctcaaccagg aagttgagat tgagcccact cattttggcg gcaccggtgt tgactccaag
    34621 agcttggtcc acacgccatc atttcctcat cggcttgaag caggaactct taatctcctc
    34681 ggtatcatcg gcatttctga aagccttgcc ttcctcttac atgaggggat tgaagccatt
    34741 cacaggagag aaatggacct gctacacaga ctccatgatg gtttttccca ttttaaaacg
    34801 atcaatattt acggacctaa gaatctttcc gatcacgttg gcctcataac cgttaacgtg
    34861 gaaggcatga gccctgaaga tgtgggctcc atactcgatg ctgactttgg cattgcagtc
    34921 agggagggtc tacactgtgc ccccctcgtt catgagagtc tgggaacatc acctaaaggg
    34981 ggtgtacggt tcagcctcgg acccttcact acggaagagg acatcgatgc aacccttgca
    35041 gcaatggcta cgattgcaag aacaaaggac tgttgttgac ctcgggtaat agttttttgg
    35101 attaatgaaa tattgaagcg tcaaaggaaa tgaaacagag agaaatatca aaaagcgatc
    35161 catacgttcc taatgacagc aaaaatctgt acgtaaacat ggaaggatgg agccacaaat
    35221 acaaaggacc ggctgcaaaa aaagcaatcc ttcaccattt ggaacattgg ccgcaagcac
    35281 gggctcttgc agccgaaatc tgtgatttct acggaaattg tttactctat tatgaagata
    35341 gcaacttgga atccagccac tatcagatca atctaaagca tgatccgata gtgtttaatc
    35401 atgcctgcgt tcaacttcag cttggtacat cacgcacggc agaagcattg tgtcacgaac
    35461 tgcttcatct cagtactcgc atgtgtggtt ttccttttgg cgaaaaattt tttatccccc
    35521 atgatcttat cgattatgca gcaacctata tgggttttta cccggtaatt ggaaatctgg
    35581 tcgaacatga acttatcttt gaaaaattca tagatattgg attcgacaaa agaaatttcc
    35641 ttggatgcat ttctcctccc ccagactata aaacactggc ctctacggct gtgaaatcct
    35701 cattttatag aaaggaaatc ggtttcccct ggtggtgttt ggagtatttc aggcattgga
    35761 tttcaatacg tcactggata ggggatgaac ctgccttgta tgcagattgc gccctgttct
    35821 ggggatcgaa agttcatccc tctatgaagg caacaacccg gaacattcgg gatttaattg
    35881 agtcaggggt gttaacagac agaaaacagt accatcgcca tgtcaacaca ctgctggaac
    35941 tgatgaaaat accaaaattt acagaatgga catttatcaa gtccggcaaa gacagcaccc
    36001 ctctggtata tcgattggca cacaaagaaa aaataaatcc caatggtagt aactcgcatg
    36061 ttttgaaaac aatgggcacg acattgaaac atggatttca atcaaatctt taaatgaata
    36121 aaaaacacgg tgaatcttgt agcggctgca cagcttaaca ttatttattg cagtcacatt
    36181 gttcacacct ataacttttc ttaacatatt ataagttttt ctttattgac atttgatgtt
    36241 ctctatagta cacaaaagat ggtattgttt tccataatag acactttatg caattcacct
    36301 aaaggcaata aactaacaaa ataggaggta attttatgaa atcttttaca aaggtgttta
    36361 taccttacgg cgggtattat tcaacacctt tttgccggtg gcagggcagc atgcagcatg
    36421 acaatgcggt ttttcttggg cccaatactg cccgaagatg gtttctcgag aaaaggaaga
    36481 ttgatcccac agttattgac tatctttact ttggaaccac tgttgctcag catcactggt
    36541 ttttttctca ccagatggca ggggctatta taaccgatga caaaaaattt gttcctggcc
    36601 tttttatcca tcaggcctgc tcaacatcaa cgacaattgt caatctcgct gcccagaaca
    36661 tagaacttgg cacgtatgag gtgtgtttcg gacttatgag cgaccggtgc tctaatggcc
    36721 ctcacacgac ttgggccaac cctcttggtc ccggaggaga gccttatcgc gaagattgga
    36781 atatggacaa tttcggtgga aacccgcgcg tgcccgttgc aatggtggaa acagctgaaa
    36841 atgtggcaag ggaagctggt ataacaaaag aggaatgcga tgccgtggtt ttaaggcgtt
    36901 ttgagcagta ccaggatgcc cttgcaaatg acagggcctt ccagaaaaga tatatgtttc
    36961 cggcgttatg cacagtaagc agaaaaaaaa caaaactcgt tgaagaggat gagggctgga
    37021 caccaacaac tgcagaaggt cttgcatcac tcaagccggt gcgccctggc ggtgtccata
    37081 gttttggcag ccagactttc cctgctgatg gaaattgcgg cataatcgtg accacaagag
    37141 ataaagcaaa agaattaagc acagacccca agattgaagt tcaggtaatc tcatacggag
    37201 cagctagagt cgaacctgcc catatgcctg ctgcccctgt tcctgccgct caaatggccc
    37261 ttgatatggc cggtctgaaa atcaccgaca tgaaagcgat caaaacccac aacccttttt
    37321 ccacgaatga tataaacctg gctaaaaaaa tgggtatcga tgttatgaaa tttaacaatt
    37381 atggatcatc cctaatctat ggtcacccac agggccccac agcggggaga ggtattattg
    37441 aaatgattga ggaacttgtg cttctcggtg gtggatacgg tctattcaca ggttgtgctg
    37501 caggagatac agctgctgct ttgattgtaa aggttggtta aaatcggcaa cattctacca
    37561 aggattttgt cggagaggct tttcttcatg gagttataga cgtatatgtg ctacaaccat
    37621 atatgtactt gggggttttt tatatcccaa aaacactata aatttaggag gtagtgtatg
    37681 gatatcaaga caattggagt actcggtgca ggatcaatgg gaaatggtat cgcccaagtt
    37741 gcggcgcagt caggttacaa tgttgtaatg agggatattg atgacaaatt cgtagacaga
    37801 gctctcaaag gtatcgataa gtttctctca aaaagtgttg aaaaaggtaa aataacctcg
    37861 gatgacaaga gcgctactct cgggagaatt aaaggaacaa cgaagatgga ggatttgaag
    37921 gatgttgatt ttgttgttga ggttgtcctt gaagacatgg atctgaagaa aaaggtattt
    37981 aaagagctgg atgagcttac ccggaaggaa gttatcctcg caacaaacac ctcctccatg
    38041 tccatcactg agattgcaac agcaacgaac agacaggata aggtcgtggg catgcacttt
    38101 tttaatccag tgcctctgat gagattggtg gaggtcattc gcggttataa caccagtgac
    38161 gacactatag ctgtagcaat ggaccttgcc aggaaattta aaaaagaacc gattgaagtt
    38221 aagaaggata taccctgctt tgtcgtaaac cgacttatga tgccccattt tctcgaagcc
    38281 attttacttc tccaggaagg catagcctct gcagaagata ttgataaggc agccaagctt
    38341 ggtctcaact atcctatggg tccttttgag ctga
//
LOCUS       DAOOMC010000007        34786 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdus sp. isolate AMR_MDS_2685
            NODE_997_length_34786_cov_6.200944, whole genome shotgun sequence.
ACCESSION   DAOOMC010000007 DAOOMC010000000
VERSION     DAOOMC010000007.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864760
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdus sp. (wastewater metagenome)
  ORGANISM  Syntrophorhabdus sp.
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae; Syntrophorhabdus.
REFERENCE   1  (bases 1 to 34786)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 34786)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,729
            Genes (coding)                    :: 2,708
            CDSs (with protein)               :: 2,708
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 7 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34786
                     /organism="Syntrophorhabdus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_997_length_34786_cov_6.200944"
                     /isolate="AMR_MDS_2685"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2699756"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            53..1093
                     /locus_tag="PK125_01300"
     CDS             53..1093
                     /locus_tag="PK125_01300"
                     /inference="COORDINATES: protein motif:HMM:NF024575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB36774.1"
                     /translation="MNSIFGHEKQKALLLSFLQKERLPHAFLFSGQSGIGKKKIALEF
                     IKHILCEKDSGCDTCRACIKIERQTHPDLLLIGPQEKKADDSPDFLEAEEKSSLTARS
                     NIPRSLIAGDKDGKVRGINQEVLAYPYEGSTRAILIDDADRMRKDAADALLKTLEEPP
                     PYNIFFLITSSEREIPLTIRSRCVRVSFAPLQREHIEEYFRKVLNVDEKKADLISMIA
                     CGSIGNGLFWLKEGNLNLRYKLAELITGKRRSFLNATGLAEQSTKTSGDLSMYLTFML
                     SLLRDMYVVLDQKDTAMVMNRDVRELLEWENIDRRWLEHAMKKVQETMTIMRYNVNRW
                     LLLENLLIHIMR"
     gene            1097..1891
                     /gene="ricT"
                     /locus_tag="PK125_01305"
     CDS             1097..1891
                     /gene="ricT"
                     /locus_tag="PK125_01305"
                     /inference="COORDINATES: protein motif:HMM:NF041131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory iron-sulfur-containing complex
                     subunit RicT"
                     /protein_id="HPB36775.1"
                     /translation="MKSCNVRIDRIAGTTEMEAPDDIRRGDYVVVEMEKGPCLGVVTN
                     TPVVTEKEGLKKIVRKATDDEVTQYYSLKENEKPALDFCREKVREMGLPMKLLFAEYL
                     FGGTKLLFYFVSENRVDFRELVKELAKEFKIRIELRQVGVRDEVKIIGGLGNCGNVCC
                     CKKFLNNFSIVSIKMVKEQSLALNPAKISGICGRLMCCLSYEYDMYMEYKKGFPKLGK
                     RIKLPQGEGKVVKHNTLSSSITVEVEDGKEITITAKDLQTLKNAGD"
     gene            1934..3460
                     /gene="metG"
                     /locus_tag="PK125_01310"
     CDS             1934..3460
                     /gene="metG"
                     /locus_tag="PK125_01310"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: protein motif:HMM:NF008900.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HPB36776.1"
                     /translation="MNKHYYITTPIYYINDVPHIGHAYTTIAADIMARYKRICGFDVF
                     FLTGTDEHGQKVEKAAEQQGISPKELADRTVHRFSDLWETLNISNTGFIRTTEERHRK
                     VVQYIFEKVFENGDIYLGHYEDWYCVPCETYFTETQLKDGMCPDCLRPTEKLKEESYF
                     FRLSKYEEKLLNYMENHKDFILPEVRYNEVASFLKGGLRDLSVSRTSFNWGIPVPMNK
                     NHVVYVWFDALANYITGIGFLDDVDQFKAFWPCDAHLVGKDILRFHTVYWPSFLMSLN
                     LEPPKHVFAHGWWTIEGHKMSKSLGNVIDPNEVVKIYGVDEFRFFLFREVPFGLDGDF
                     SKHGIVNRINGDLANDFGNLASRSVTMIGKFLKGKIDRPEKKGGMDEYVEDNVRRLVE
                     EYQKEMEVFAYHKALQNAFEIISILNKYIDTEAPWRLAKEGDNRIKTVLYNIWNSLRI
                     VAMLLYPYMPSKSQAIWNALGIGKPIDKTLFDNEKIFYFMDDLGTIDKINPIFPRIEG
                     "
     gene            3462..3758
                     /locus_tag="PK125_01315"
     CDS             3462..3758
                     /locus_tag="PK125_01315"
                     /inference="COORDINATES: protein motif:HMM:NF017103.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF721 domain-containing protein"
                     /protein_id="HPB36777.1"
                     /translation="MGFHSLQRVLKRVLKDCNLPAHNIDAYKVFHLWEKIAGEGLANH
                     AKPVRIDNRILYVEVDDPIWLSQVRYMKLDIMAKIGVTIKKGIFKDIRFFLKNS"
     gene            complement(3840..4991)
                     /locus_tag="PK125_01320"
     CDS             complement(3840..4991)
                     /locus_tag="PK125_01320"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HPB36778.1"
                     /translation="MNFDLNEEQTMVRKMAHDFAEKEMLPTLLDCERRREVNLTLIQK
                     MASQGLLGVHLPQKYGGMEAGYVTASIVWEQLSRASWTQTLISLGHAVLAGTILSKVA
                     NEEQKQKYLPDLCNGSLIVAMAAVEPNVGSDASAVETKATLDGDTWIIDGSKNFITAG
                     NLADVVLVLVQTNKDLGPKGLALIAVEKEVSGFTATPAEMVGGWASDISNLSFMECRV
                     PKANLIGDIGRGLQNALIGIDTARLFVAAGAIGMAQSCLDACVKYAKERYQFKRPIGG
                     FQLIQETIARMYAEIEATRWQVYYAADLKSKNAPHAKELSAAKWLSSELAVRVGAEAI
                     RLHGAYGCTNEYLVEHHYRDAIMSTILGGTSEMHKLTIGRELLGINALS"
     gene            complement(5003..6559)
                     /locus_tag="PK125_01325"
     CDS             complement(5003..6559)
                     /locus_tag="PK125_01325"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013225.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HPB36779.1"
                     /translation="MKERIKRLREVKKTASMGGGAEKIAKEHEKGKLSARERINLLLD
                     PDSFNEFNMLVKYRMGAPGDGIVVGHGTIDGRRICVFAHDATVMGGSQGYMHGRKLYK
                     IHEMALDMGVPLIGLNDSPGARVIRHEVAGSDDPYRVSDEKHAGVVFFMNARCSGVVP
                     QISAILGNCTGGSVYSPALTDFIFMVDGLSHMCITGPRVIKSVMHEDVTLEELGGAKI
                     HCQVTGVADFRVKSEEDCMASIRKLLSYLPQNCTTRPERKDTQDDPGRMTAMLEEIVP
                     EQPTQGYDMHRVIETIVDEGDFFEIKREFAGEIIVGFARIDGRVVGIVANQPMVKAGC
                     MSVDSSDKQARFIRFCDAFNIPIVLLVDTPGYLPGKGQEHAGIIRHGAKVLYALSEAT
                     VPKIAILLRKVYGGAALGMGIMPGFGTDLIFGWPTAEVGAMGAAQAVDLFYGDEIKKA
                     QDPQEYRERKIQDYKDLYADPLALASDVTWVQDIIEPAETRRCLVRSLNLIENKGITR
                     NARRHGNIPL"
     gene            complement(6573..6800)
                     /locus_tag="PK125_01330"
     CDS             complement(6573..6800)
                     /locus_tag="PK125_01330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB36780.1"
                     /translation="MEKDKKKSIYAEKRIFQFPTQERQLLVPDEDEMFRTGAEKPAGE
                     MIRVNANLSKKPCGEILMEEPSPKVFVAKSI"
     gene            7366..8418
                     /gene="msrB"
                     /locus_tag="PK125_01335"
     CDS             7366..8418
                     /gene="msrB"
                     /locus_tag="PK125_01335"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000290139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HPB36781.1"
                     /translation="MGEVFQNTKSAVFAGGCFWCTESDFEKVEGVIDVISGYTGGTMP
                     NPTYEQVCTGNTGHLEAVKVIYDPSKITYEELLDIFWRHIDPTDAGGQFTDRGTQYGS
                     AVFYTTEEERKSAENSKKNLAAMGPFTEEIRTEILQLGPFYPAEPYHQNYYKKNPSHY
                     NAYRSGSGRDRFLKEVWAERKSTESSGIKKETKKNVATTEKIASPYLIPSDTELQKRL
                     TPLQYHVTRQDGTEPPFNNEYWDNHNEGIYVDIISGEPLFSSTDKFGSGTGWPSFTQP
                     LEPENIVKKTDRGFLMVRTEVRSKHANSHLGHVFDDGPKPKGLRYCINSAALRFIPKA
                     ALEKEGYGKYQYLFSR"
     gene            complement(8582..10933)
                     /gene="lon"
                     /locus_tag="PK125_01340"
     CDS             complement(8582..10933)
                     /gene="lon"
                     /locus_tag="PK125_01340"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: protein motif:HMM:TIGR00763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HPB36782.1"
                     /translation="MVIGFRNDKTLKDKIFIPAELPILPLRGTVAYPDLVMPLIVGRD
                     RSIKLVDEANAGDKMIGIITQKNPDIEDPGIDDLYMIGTVATIMKMVKMVDGSQRIVV
                     QGICRFKLVEFTSTEPHLRAKILPIFEEYQKDIEIDAMYLNLKNLYKKAVEMAPYLSS
                     ELSQIASQMENPGNLVDLIGSTINIGVAEKQEILEKIDLKERLKKVTILLNREVETLE
                     LSSRIQSHVKEGIDKTQREYYLREQLKAIQKELGETDDKMSEMEEIRKKILEARMPPD
                     VQKIAEKELDRLSKMSNMSAEYTVSRTYLDWLTEIPWSQATEDNLNIQDASKILDEDH
                     YDLTKVKKRILEYLAVRKLKADMKGPILCFAGPPGVGKTSLGKSIARALGRKFMRISL
                     GGIRDEAEIRGHRRTYVGALPGRIIQGVKKAGSNNPVFMLDEVDKIGMDFRGDPSSAL
                     LEVLDPEQNFSFSDHYLEVPFDLSKVMFIATANMLDPIPPALKDRMEVLELPGYTEEE
                     KLMIAKQFLIPKERNEHGLTEDLIEFQDDALKVVIKSYTKESGVRNLEREIATICRAV
                     ARDVAEGTTEKKIITADSIHGYLGPIKFFSEVAERVKYSGVATGLAWTPTGGDILFIE
                     STKMRGKGNLTLTGQLGDVMKESAQAALSYIRGKAADFNITEDFFEKNDLHVHVPQGA
                     IPKDGPSAGVTMLVSLVSLLTDRHVRNDVAMTGEITLRGLVLPVGGIKEKVLAAKRAG
                     IKSVILPKLNEKDLEEVPESIKENMEFKFIERMDEAVSICLTA"
     gene            complement(10943..12859)
                     /gene="dnaK"
                     /locus_tag="PK125_01345"
     CDS             complement(10943..12859)
                     /gene="dnaK"
                     /locus_tag="PK125_01345"
                     /inference="COORDINATES: protein motif:HMM:NF001413.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HPB36783.1"
                     /translation="MAKKVIGIDLGTTNSVVAIMEGGEPKVIINEEGNRLTPSVVAFT
                     KDGEILVGQTAKRQAITNPENTIFSIKRFVGRRYNEVQSEIKTVPFKVVSDSEGLARV
                     EVRGKQYTPQEISAFVLQKLKKSAEAYLGQTIEDAVITVPAYFNDSQRQATKDAGRIA
                     GLNVLRIINEPTASALAYGLDKKKDETIAVYDFGGGTFDISILEVGDNVVEVKSTNGD
                     THLGGDDIDQRVIEWIVAEFKKDQGIDLAQDKMALQRLKEAGERAKIELSTAIETEIN
                     LPFITADASGPKHLNLKLRRAELEKMCDDIIQRTVEPCKRALEDAKLSPGKIDEVVLV
                     GGSTRIPKVQEVVKNFFGKEPHRGVNPDEVVALGAAVQAGVLAGEVKDVLLLDVTPLS
                     LGIETLGGVMTRIIERNTTIPTKKSQVFTTAEDSQTSVEIHVLQGEREMARDNRTLGK
                     FHLIGIPPAPRGIPQIEVTFDIDANGILHVSAKDLATGKEQNITITASTGLSEDEIKK
                     MVHDAETHSEEDKKRKHEIEIKNQLDNMIYTTEKTLNESKDKLSADDIRSMEDVLTRA
                     KETVKTGDADRIKSAVDELTKASHKLAETLYKRTSETTSKGPGPEGAQTQSEAGGGRR
                     DDDVVDAEFEDVKK"
     gene            complement(13036..13848)
                     /locus_tag="PK125_01350"
     CDS             complement(13036..13848)
                     /locus_tag="PK125_01350"
                     /inference="COORDINATES: protein motif:HMM:NF013143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HPB36784.1"
                     /translation="MDIINLLSHGFIQRALMAGSFIAILCATLGTFLVLRRFSLIGDG
                     LAHVTFGGVAVGLFLNVYPIYAAVPVAMISSLGILKLVEKARVYGDAAIGIVSSLGIA
                     CGILLASVSGGFNIDLFSYLFGNILAISNAEVISSIFLSLILMSVIWYYYHELLSITF
                     DEESARASGIKTKTINGILVLLTSLTVVLAMRVVGIMLISSLLILPPVTALQIARGFK
                     MTIVIASVSGVLSIILGIFFSLVFDLPSGATIVLLNFLLFVCSFVYGRLLVK"
     gene            complement(13998..14756)
                     /locus_tag="PK125_01355"
     CDS             complement(13998..14756)
                     /locus_tag="PK125_01355"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HPB36785.1"
                     /translation="MSTIDALSVHDLSFQYNATEILSDISFHLQVGDYLGIVGPNGSG
                     KSTLIKLTLGLLLPSKGVIKIFGTKTADFVDWHKIGYLPQRINSFNPHFPATVREVVA
                     LGLLSTKSNPRRITKNDEKAINNVLELTEILDIKNKLIGELSGGQQQRVLIARALINE
                     PALLILDEPTTALDPEAREKFFSTLKDLNEHKKVTVIIITHDIGTIGKYASRLLYLDK
                     QVVFYGGFDDFCESNDMASYFGEYSQHIICHRHD"
     gene            complement(14744..15727)
                     /locus_tag="PK125_01360"
     CDS             complement(14744..15727)
                     /locus_tag="PK125_01360"
                     /inference="COORDINATES: protein motif:HMM:NF013465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HPB36786.1"
                     /translation="MTTNAVPSSILIFSLILFLVGAGCQHREQEAVEAKKLSVVTTLF
                     PLYDFTKNIAGSKARVTLLMPPGVEAHSFEPKAGDVLKVNNADVFVFTGQYMEPWAYG
                     LLRGVDKRELVVVDASKGITLEEGKQEHGDHKDEKGEKDRTGGSHAHGKIDPHIWLDL
                     ANAQKMVANILGALLTVDPDNKDFYRKNADAYIMKLADLDARYKKGLFACKKNIFVHG
                     GHFAFNYLAKRYNLRYISAYPGSPDTEPSPWQIISLKKKIRENHIQYVYYEELIAPRM
                     ANILAKETGARLLRLHGAHNISKEDFEKGVSFLSIMEENLKNLKVGLECQQ"
     gene            complement(15737..16168)
                     /locus_tag="PK125_01365"
     CDS             complement(15737..16168)
                     /locus_tag="PK125_01365"
                     /inference="COORDINATES: protein motif:HMM:NF013629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HPB36787.1"
                     /translation="MDTNRIVKELGLKATPKRIAILEILAEAFCYLGPEEIWRKLQTR
                     FKRIGLPTVYRNLEELSNGGVIIKIIHPNRKLYYYYCNNTSHHHHFVCIACRRVQDLE
                     FCGEEKIIREVEDTLKGKVVSHLFQVYGLCSDCTLSHAVPT"
     gene            complement(16599..16886)
                     /locus_tag="PK125_01370"
     CDS             complement(16599..16886)
                     /locus_tag="PK125_01370"
                     /inference="COORDINATES: protein motif:HMM:NF012790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HPB36788.1"
                     /translation="MRLRRLTLPLIFASALFLFYCGIKPDIPDLSADELMRMMNGETN
                     LMVIDTRTQIEYARGRIPRSLFVPEEKFYALKLILPPAKETLLVFYCRGPG"
     gene            complement(16888..18345)
                     /gene="thrC"
                     /locus_tag="PK125_01375"
     CDS             complement(16888..18345)
                     /gene="thrC"
                     /locus_tag="PK125_01375"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HPB36789.1"
                     /translation="MEPITYYSTNNPNERINFEDALLRGMAPDYGLYMVPRKELPRIS
                     HEKLMAMPFMYYPQIAFEVLSPFLRSEVPEKVLRSLLNDAYREDVIPTDIQQVTGRTH
                     IMWLTKGPTYSFKDYAARFFGRMLNYFLGVRRLRRVVVVATSGDTGGAVADALLGLKN
                     IDNIVFFPKGSISEGQRRQMTTLKNNIYAFEINGDFDVCQALAKTILGDQSFAEELFN
                     DRERFTSANSISLGRLLPQVVYPFYAYSRIADNMEPIIASIPSGNFGDMMGTVLAKGM
                     GLPVSKIIAGVNENTEFPEFLETGQYEVRPSIKSPSSAMIVSHPSNLARLIDFYGGHM
                     YDERDRVTGKIIKPGVIDRMPDMDEMRKDIVSMGVTNPQHFETMKEVYEKYGVVLDPH
                     GAVGWKVLEVCLGGRHDEPAVVYETADPGKFPEDVERAIGVEPDLPPGMQRQATMEER
                     IYSIESTPEQEHGGLKLSQAQIDETKARIKEIFKR"
     gene            complement(18535..19875)
                     /locus_tag="PK125_01380"
     CDS             complement(18535..19875)
                     /locus_tag="PK125_01380"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HPB36790.1"
                     /translation="MNLPKIIEKNEIKIFLRDSWSISWPMILIMFFEFLTGLVDIYIA
                     GQIGKEIQAAYGFIIQLYFVFIIVGNALTVGAVSIISRLFTAGNKDEITEAIYSTLSI
                     TIIAGVFLGIGGFFLTGNIIALLNIPQEVKPLAMPLGVIYSAGLPFHYVLIQTNGILR
                     ACKMAKTSMKTMVIVCACNISLAFLLVFNTSLGYKGIAVATAVSVCIGSFINSFHVWN
                     LMDGIKRFSRAVSKSVMNIGWPSGLSQVLWQAHSMAIFLILSSLPKNNVEILAALSAG
                     IRIESAIFLPAFAFNMANAVVIGNFLGEKKTEDAFRGGIITACLGVPIVIALTLAVIL
                     NARWIAPILSNNGIVVAETIRYLYINMLSEPFMAWAIILGGGLMGAGDTRSVMVLIGM
                     SIWLVRVPLSYLFVVILGFGAISVWWVMNISQLVMAFLISRRYFSKKWLNVKQL"
     gene            20230..21426
                     /locus_tag="PK125_01385"
     CDS             20230..21426
                     /locus_tag="PK125_01385"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF005305.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HPB36791.1"
                     /translation="MIISNEMDQLIKDQEGWTRRMFEEGIRIKKLYGLDAVCDFSLGN
                     PDTEPPETLRKALIDMLSSPEVGTHRYMSNNGYEDVRENIAEYLREVHGVPFTAGHIF
                     MTVGCAGGLNVILKSMLNPGDEVIVPTPFFWEFKNYINNFSGVTRFVETKEDFQLDIG
                     NIEAAITKKTRAILINSPNNPTGAVYHEESMKQLASLLNTKRELGQDIYIITDDAYKK
                     LVYDGIQIPNIFKMYDLVLSVTSHSKDLALAGERIGYVAISPSIKDMDLLVSAMTISI
                     RVLGFVNAPALFQRVAGKFQRTSVSIADYEKKRDVLYAILMEAGFECVKPQGAFYMFP
                     KSPIPDELEFVRTLQQEERIMVVPGRGFGREGYFRIAYCVPMEKILKAREGFLRTGDR
                     YYRGGK"
     gene            21423..22616
                     /locus_tag="PK125_01390"
     CDS             21423..22616
                     /locus_tag="PK125_01390"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF005305.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HPB36792.1"
                     /translation="MMPISKNISTLMKNSSWIRAMFEEGEELKKKFGTDNVCDFSLGN
                     PITEPPEALKNELIKVASSDAQGMHRYMTNSGYDNVREEVAQYQREITGLPYTRDHIV
                     MTVGAAGGLNVIFKSILDPGDEVIVPSPFFVEFMFYIGNHNGIIKLVDTKDDFHLDID
                     KIKAAISRKTKAILINSPHNPTGVVYNEAELLELADLLHERSKKGQRIYIVADEAYKK
                     IIYNGIKLPNLPNMYEDTVNVTCHSKDLGLAGERIGYIAISPRIKNVRSLIDAAIFAN
                     RILGFINAPALMQRVVAKFQHNSVDIMEYQKKRDAIYNILVESGFEVIKPEGAFYIFP
                     KSPIPDDIQFVKTMLQHRILTVPGVGFGKSGYFRLAYCVTMDIIEKSRPHFKEVGELL
                     RKGQK"
     gene            complement(22777..23493)
                     /gene="bioD"
                     /locus_tag="PK125_01395"
     CDS             complement(22777..23493)
                     /gene="bioD"
                     /locus_tag="PK125_01395"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: protein motif:HMM:NF024891.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004141 - dethiobiotin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HPB36793.1"
                     /translation="MKVIFVTGTDTGIGKTVISSAVSAFFSLRKKMNVGVMKPFECGL
                     SKTDKDLLPWDAICLREASGSTDGLNIISPYTLEAPCAPEVAAMIEHVSIDISLVDKI
                     FQGLKRSHDLLVIEGAGGVLVPIREHFFFIDLIQKWNAPTLVVSRLGLGTINHTLLTI
                     HLLKERGIKVLGVILNDNAGSDDLPTQTNPTILKKYLADIPILGIFPHVKDLIKGNVD
                     RERMADILAKNINTDPLLEQ"
     gene            complement(23493..23702)
                     /locus_tag="PK125_01400"
     CDS             complement(23493..23702)
                     /locus_tag="PK125_01400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB36794.1"
                     /translation="MWRVLSNIIEMFVYSAVYIIIVLIMLKVFGATFTSEFEKKISED
                     GNMALSILCAGLLIGLSLLLASIIR"
     gene            complement(23712..24353)
                     /locus_tag="PK125_01405"
     CDS             complement(23712..24353)
                     /locus_tag="PK125_01405"
                     /inference="COORDINATES: protein motif:HMM:NF003989.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HPB36795.1"
                     /translation="MSKYPKIPGATIRRLSNYLKALEDLESKNEKVASSALLAEICNV
                     NAAQVRKDFAYFGEFGIRGMGYNVKELKFHIKEILGVNREWRIAVIGIGNMGSALLVY
                     KDFLKQNYKIVAAFDIDPVSVIGRISERMGKPVEILHVDMLKEVIKARNIEIGIITTP
                     PGAAQQIADLLSETEIKGILNFAPLPVRSHGNIPLRNIFFTAALDNLVYYLSN"
     gene            complement(24479..25273)
                     /gene="dapB"
                     /locus_tag="PK125_01410"
     CDS             complement(24479..25273)
                     /gene="dapB"
                     /locus_tag="PK125_01410"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HPB36796.1"
                     /translation="MIKTIITGVCGRMGSMILKLALEDPDFMVSGVTEIEHHPSVGKC
                     AEAVIVGAGNALIVEGSLASVIDRGDVVIDFTEPEATLENFRLVKKKAKAIVIGTTGL
                     KGQGLDEIMKTKNTRIVISPNMSVGMNLMFDVVEKLSKKMKDHYDIEIFEMHHKLKKD
                     APSGTAVKLKDLIKNAAPDRKWIEVFGRQGMLGERKKEELGIMSLRGGDVVGEHTVMF
                     AGIGERLEITHRAFSRENFARGALVAARWLVKQKPGVYTMKDVLGL"
     gene            complement(25302..26189)
                     /gene="dapA"
                     /locus_tag="PK125_01415"
     CDS             complement(25302..26189)
                     /gene="dapA"
                     /locus_tag="PK125_01415"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HPB36797.1"
                     /translation="MELKGIYTALVTPFKNYDVDEEALERLIDFQLKSKVDGLVPCGT
                     TGEAATLSYEEHEKVVELTVKHVKGTVPVVAGTGSNSTKETIELSQSAKNVGADMCLL
                     TTPYYNKPTQEGLYQHYKKVAEEVDIPIILYNIPGRTGINMNPDTIARLAQIPNIVGI
                     KEASGSLVQVAEIYRLAKERITIMSGDDNLFLPMMSVGARGVISVLSNAMPVELQALY
                     QAFLGEKDMQKAMDMNTSLLPIMQAMFVETNPIPIKETLYHMGMMEKEFRLPLCPLSE
                     GSSKFLKGVLREYGLLKRE"
     gene            complement(26203..27015)
                     /gene="dapF"
                     /locus_tag="PK125_01420"
     CDS             complement(26203..27015)
                     /gene="dapF"
                     /locus_tag="PK125_01420"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HPB36798.1"
                     /translation="MLDLQFFKMSASGNDFIIIDNRDGRVYDNFKEPKEFVVKVCRRN
                     HSVGSDGLILIEKSAMCDFRWRFFNADGSEAEMCGNGGRCAARFAYINGIAKEKMSFE
                     TIAGIIKAEVDDARVKLQLTSPTDIKLDYPVGLEDKEIFLSSVNTGVPHAVLLADDVD
                     HMPVEELGRLVRYHKAFGEKGTNVDFVQIVDKKNVKIRTYERGVEAETYACGTGAVAA
                     GVILKAKGLLKSPMNILTRGGETLKVYIDEEVYLEGVTKIIYVGKLHEEALM"
     gene            complement(27020..28279)
                     /gene="lysA"
                     /locus_tag="PK125_01425"
     CDS             complement(27020..28279)
                     /gene="lysA"
                     /locus_tag="PK125_01425"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR01048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HPB36799.1"
                     /translation="MNYFDYKNEELFGESVPVAQIAQEVGTPVYIYSYKTLERHYRVF
                     DSAFNRVPHLTCYSCKANSNIAILRVMGFLGGGADIVSGGELYRALRAGIQPQRIVFS
                     GVGKTEEEIISAVKVDILMINVESAEELSVIQRVAKNMRKKIPLSVRVNPQIDAKTHP
                     YITTGLKKNKFGVLWGEAYRLYHEMKSDPYLDPIGISSHIGSQILELGPFVEAVRSLK
                     KMVMQLRGDGIPVAYMDIGGGLGITYKDELPPHPGEYGKAIGQELEGADLTIILEPGR
                     VLVGNSGILVTKLLYKKTVPGKTFYIVDAAMNDLVRPAFYDAYHEIVPVSRKTSREIK
                     ADIVGPICESGDFFAKSRQIADLESGELLAIKGAGAYGFSMSSNYNSRRRAAEVLVKG
                     KEFFIIRKREVFKDLIRGESVPSFLEE"
     gene            complement(28288..29289)
                     /locus_tag="PK125_01430"
     CDS             complement(28288..29289)
                     /locus_tag="PK125_01430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB36800.1"
                     /translation="MSRKDIILILSIGILCLVFSCGKKGNPLPKQQPVPAGAETLSGE
                     VKDGVLFLSFAMPIKNLDGSDAVDLAGFKILKRCVTCEGAFEAFREIRLEDEKGYTMA
                     GNKIFIYDDDLYHGYTYSYMVKPFTTRGVLGESSKIFTIRWEDPPTKPAGNVTVREND
                     GRVELSWHQEKGFFYNVYRSENGFYQLFPINKDPLSSPFFLDAGLKNGQKYVYEIRKV
                     KVVEKRVLEGEGIRVEATPKDLTPPAVPRGIKAEKRGSVVQISWLENKEDDMAGYNVY
                     RVVGRLEQKLTKTPIKETQFMDHNLGEHRFLSYYVKSVDISGNESEPSREIIVIVKE"
     gene            complement(29258..30631)
                     /gene="argH"
                     /locus_tag="PK125_01435"
     CDS             complement(29258..30631)
                     /gene="argH"
                     /locus_tag="PK125_01435"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HPB36801.1"
                     /translation="MKAWGGRFKGKTEPLMERFSASIGFDWALYEYDIEGSKAHAEML
                     HKIGILDEKEREIIINALEEVKREIQEGEFVFSNALEDIHMHIEARLIDKAGEIGKKL
                     HTGRSRNDQVALDMRMYLKKELGEIRNLLRNVLHSFITRAEKEKETIMPGYTHMQRAQ
                     ITTFAHHLLAYYQMLKRDLERLIQIWKTVDVLPLGSGAIAGSTIPLDREFVRERLNFS
                     SISENSMDTIADRDFVLESIYSIAMIMIHLSRLAEDLIIFSTDEFAFVSLPDELCTGS
                     SLMPHKKNPDSLELIRGKTSRVIGDLFSIFSLLKGLPLTYNRDLQEDKEPLFHAISTV
                     KDVLSIVELCIKGMSVNREKMKEAVYESFMPAVEMAEYLTLKGIPFRQAHVIVGKLVR
                     DCEDKGKQLRNLKLEDLRTYSEAFDKDIIAHIDPLTILRNRKTMGAASFEEVERQIVA
                     ERHYLNS"
     gene            complement(30628..31299)
                     /locus_tag="PK125_01440"
     CDS             complement(30628..31299)
                     /locus_tag="PK125_01440"
                     /inference="COORDINATES: protein motif:HMM:TIGR00044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HPB36802.1"
                     /translation="MNTIEDGIQRIRERISRASIRSGRSEKDVRLIAVTKRVDVERIR
                     IAFSCGIRDLGENYIQEARRKIDDFGEGPCWHMIGHLQTNKVKYVPGLFSYVHSVDRW
                     EIIERLEKFGKPMKVLFELNLSGEQSKHGTQEDNLKRMLERIHTLQNIEPVGLMTMPP
                     FTENAEDVRGIFSGLRQILERVNKEFSLSMTELSMGMSSDFEVAIEEGATMVRIGTAI
                     FGERQ"
     gene            complement(31296..31886)
                     /locus_tag="PK125_01445"
     CDS             complement(31296..31886)
                     /locus_tag="PK125_01445"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HPB36803.1"
                     /translation="MFQVKDGDSIVLASESTRRVDILRSLGISFSIIPPDVDETKHRD
                     ETYREFVERISYEKARKVGKHFPDKWVIAADTIVVLKGKVLGKPKNERDAFNTLKTLG
                     GKWHKVITGFCVLNVLQDITYCNIVETKVFMKNMTDDEIIRYIKTSEPMGKAGSYAVQ
                     GRGGYMVKEIKGSYTNVVGLPICEIAEALLSLGVLS"
     gene            32016..32357
                     /gene="cutA"
                     /locus_tag="PK125_01450"
     CDS             32016..32357
                     /gene="cutA"
                     /locus_tag="PK125_01450"
                     /inference="COORDINATES: protein motif:HMM:NF015077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010038 - response to metal ion [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="divalent-cation tolerance protein CutA"
                     /protein_id="HPB36804.1"
                     /translation="MDKIIQVITTCNNKELLEEIGTYLVENRLAACAQILGPITSTYW
                     WQGEIEQATEWVCTLKTTEGLYEKVETAILELHPYDLPEIIAIDVEKSLPAYADWVRD
                     ETGQGNKGNTI"
     gene            32354..33934
                     /locus_tag="PK125_01455"
     CDS             32354..33934
                     /locus_tag="PK125_01455"
                     /inference="COORDINATES: protein motif:HMM:NF013086.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HPB36805.1"
                     /translation="MRNHVIIIGAGLAGMAAAMSAHDKGASVTLIDRGSIGLGTNSAL
                     AGGQFTGPTFLHSPEEFIQDTLDTGRGINRKRLVRLVAFKAPEAFDFLRHLGLKLIET
                     PTAFRIQSPNPVVIPGVTLVRTLAEKIKEMKHIQILTRFYVTKIIIENGKATGVCGFD
                     KEGRNRTIHGDGIVLATGGAGAIYSKNDNQKTIMGQGYYLASSAGLNLWDMEFVQCYP
                     FVIAEPHLPSLLVYPPYPHEARLVNGKGEDILARYGIEDINDAIMKKRDELSVILNEE
                     STVSRVLMDFSEVPDHMWDAYPLSILEKLHFDVRKKPFAVSPAVHFCMGGIEVNEKGQ
                     TSLEGLYACGEVTWGLHGANRRGGNALTECLVMGRVAGTFAARYRGEEESEDHDNMNH
                     QNEHADSAQGGLFISFRELRAHVRNLAWEHAGIVRDEAGLKKGLLMLDSIEGELKNAM
                     WTNVRERIIKEDLLSAVFSVKAILSASMGRKESRGSFIRKDYPKEDNDNWRKNSCLTY
                     NPQNNSFSVSYHDAEWGD"
     gene            33971..34726
                     /locus_tag="PK125_01460"
     CDS             33971..34726
                     /locus_tag="PK125_01460"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HPB36806.1"
                     /translation="MKLKNMVAFCTAAAGAGIGHSTARTFAREGASIVITDVHLERCR
                     AVADEIQHDFGVDALAIKCDVTNREDVEAAINRTLKQFDRIDILFNNAGTNRPSQIID
                     ITDEVWDLVVNTSLRGTFYCCRAVLPAMIKQNFGRIVSTTSVAAFRGLKAGHAHYAAA
                     KAGVMAFTRCLAMEAAPYHITANTIAPSFIYNEFIPHIYPQDEIDRMYDEIPYPRKGT
                     PEDIANTVLFLVSQEGEYITGQTICVTGGSWMR"
BASE COUNT         9531 a         8208 c         7263 g         9784 t
ORIGIN      
        1 gtacacagga tcattaagga gaaagtcgaa tcacttctca tgagccatgg gattgaatag
       61 tatttttgga cacgaaaagc agaaagccct gctcctctca tttctccaga aagaaaggct
      121 acctcatgcc tttcttttct caggccagag tgggattggc aaaaagaaaa ttgcccttga
      181 attcataaaa catattctct gcgagaaaga tagcggttgt gacacctgtc gggcgtgcat
      241 caagattgaa cggcagacac acccggatct tctccttata ggaccccagg aaaaaaaagc
      301 cgatgacagc cctgatttct tggaggctga ggaaaaatcc tctctaaccg caaggagtaa
      361 cataccacgt tcactcattg ccggtgacaa agatggtaag gtaagaggca ttaaccagga
      421 ggttcttgca tacccttatg aaggatcaac acgggcaatt cttattgatg acgcagatag
      481 gatgagaaaa gacgctgctg atgcactcct taaaaccctt gaagaaccac ccccttacaa
      541 catcttcttt ctaataacgt catctgaaag ggaaattcct cttactattc gttcacgatg
      601 cgtaagggtt tcctttgctc ctctacagag ggaacacatt gaagaatatt tcaggaaagt
      661 actcaatgtt gatgaaaaaa aggctgatct tatctcgatg atcgcatgtg gtagcattgg
      721 aaacggactg ttctggctga aggaagggaa tctgaacctg agatacaagc tcgctgagct
      781 cataacaggt aaaagaagaa gtttcctcaa tgcaacaggt ctcgctgagc agagtacaaa
      841 gacctccggt gatctttcaa tgtatcttac cttcatgctc tctcttttaa gggatatgta
      901 tgtggtccta gatcagaaag acactgcaat ggtcatgaac agggatgttc gagagcttct
      961 tgagtgggaa aatatcgacc ggaggtggct ggaacatgcc atgaaaaagg tgcaggaaac
     1021 catgacaatt atgcggtata atgtaaaccg ctggttgtta ctggaaaact tgctgattca
     1081 tatcatgagg taacttatga aaagctgtaa tgtgaggata gacagaattg caggtaccac
     1141 agaaatggaa gcgcctgatg atattagacg tggtgattac gttgtcgttg aaatggagaa
     1201 ggggccgtgc ctcggagtcg tcacaaatac tcctgtagtc actgagaagg aggggctcaa
     1261 aaagatagta cgtaaagcaa ctgacgatga ggttacccag tattattcct tgaaggagaa
     1321 tgagaaacct gcccttgact tttgtcgtga gaaggttagg gagatgggac tccctatgaa
     1381 acttctcttt gccgaatatc tctttggggg aacaaagctt cttttctatt ttgtatctga
     1441 aaacagggtt gattttagag aactcgtaaa ggaactcgca aaggaattca agatcaggat
     1501 agagctcaga caagttggtg tcagagacga ggtgaagatc atcggtggcc ttggaaattg
     1561 tggtaatgtt tgctgttgca agaaattctt gaacaacttt tccatagtct ccattaaaat
     1621 ggttaaggaa caaagccttg cattgaaccc tgctaaaata tcaggtattt gcgggagatt
     1681 gatgtgttgt ctttcttatg agtacgacat gtacatggaa tataaaaaag gttttcctaa
     1741 actcggcaaa cgtataaagc tccctcaggg ggagggcaag gtggtcaaac ataacacgct
     1801 cagctcatcg atcactgtag aggttgagga tggcaaagag ataacaataa ccgcaaaaga
     1861 tcttcagact ctgaagaatg caggggatta aagactgaag gctacggata agctttggag
     1921 gtaaaggtaa ctgatgaaca agcactatta cattacaacg cccatttact acattaatga
     1981 tgtcccccat attgggcatg cctacacaac aatcgctgcc gatataatgg cccgttacaa
     2041 gaggatttgc gggtttgatg ttttttttct tacaggcaca gatgaacatg gccagaaagt
     2101 tgaaaaagca gcagagcagc agggtatttc cccgaaggag cttgccgata gaacggttca
     2161 caggttttca gatttgtggg aaactctcaa tatatcgaat actggtttca tcagaaccac
     2221 tgaagagaga cacagaaaag ttgtccaata tatcttcgag aaggtatttg aaaatggaga
     2281 catttacctt ggtcactatg aagactggta ctgtgtccct tgcgagacct atttcacaga
     2341 aacacaacta aaggacggaa tgtgtcctga ttgccttaga cccactgaaa aactcaaaga
     2401 agagagttac ttcttcaggc tttccaaata tgaggaaaaa ctccttaact acatggaaaa
     2461 tcacaaggat ttcattttac ccgaggtacg ttacaacgaa gttgcaagtt ttctgaaagg
     2521 aggtttacga gacttaagtg tgagccggac aagctttaac tggggcatac ctgttccaat
     2581 gaacaaaaac catgttgttt atgtctggtt tgacgccctg gcgaattaca ttaccggcat
     2641 aggatttctc gatgatgtgg atcagtttaa agcattctgg ccatgcgatg cccaccttgt
     2701 tggcaaggat atacttcgtt tccacactgt atattggcca agctttctca tgtccctcaa
     2761 ccttgaacca ccaaaacatg tttttgccca cggatggtgg actattgaag gacataagat
     2821 gtcaaaatct cttgggaatg ttattgatcc caatgaggtt gtcaagatat acggagtgga
     2881 tgagttcagg ttttttcttt tcagggaggt gccttttggc ctcgatggtg atttttcaaa
     2941 acatgggatt gtgaaccgta ttaacggtga ccttgctaat gatttcggaa accttgcgag
     3001 cagaagcgtg accatgatag gaaaattcct caaagggaaa atcgataggc ccgagaaaaa
     3061 gggtggtatg gatgaatacg tggaagataa tgtaaggcgc cttgttgagg aataccagaa
     3121 agagatggag gtgtttgctt atcacaaagc cttacagaat gcttttgaaa taatatctat
     3181 acttaataag tatatagaca ctgaggcgcc atggagactg gcaaaagagg gagataatcg
     3241 tatcaaaaca gtgttgtata atatttggaa tagcttacga atcgtggcaa tgttgctcta
     3301 tccatatatg ccttcaaaat ctcaggctat ctggaatgct ctcggcattg gcaaacccat
     3361 tgacaaaact ctttttgaca atgagaagat tttttacttc atggacgacc tcggaaccat
     3421 cgacaaaatc aacccaattt tcccaaggat agaaggatag aatgggtttc cactccctcc
     3481 agcgagttct gaagagagtt ctcaaggact gcaatcttcc tgcccacaat attgatgcat
     3541 acaaagtatt ccacctctgg gaaaaaatag ccggagaagg tcttgccaat catgcgaagc
     3601 cagtaaggat cgataacagg attctctatg ttgaggtaga tgatcctata tggctttccc
     3661 aagtgcgtta catgaaactc gacattatgg caaaaattgg ggtaacgatt aaaaaaggga
     3721 tcttcaaaga catcagattc tttctcaaaa atagctaaaa gggttggccc tgtcccacca
     3781 gggcctgtgc ctgacacctg actcctttgc tacaaaagaa aatgtttttt tcactaaaat
     3841 caggacaacg cattgatccc taacagttct cttccgatgg tcagtttgtg catctctgac
     3901 gttcctccga ggatggtact cattattgca tccctataat gatgttccac aagatattcg
     3961 ttcgtgcatc cgtacgcccc atgcaaccgg atagcctccg cgcctacccg cacagcaagt
     4021 tccgaagaca gccattttgc agcagaaagc tccttggcgt gaggagcgtt tttacttttc
     4081 aggtcagcag cataatagac ctgccagcgt gtagcctcaa tctcagcata catacgcgct
     4141 attgtttctt gaatgagttg aaatcctcca ataggccttt tgaattgata acgttctttg
     4201 gcgtatttta cacatgcatc aagacatgat tgggccatcc cgatagcacc agcagcgaca
     4261 aaaaggcggg ccgtatcgat gcctatcagc gcattttgaa gacctcgacc aatgtctcca
     4321 atcagatttg ccttcggaac ccggcactcc ataaaagata agtttgaaat atcactggcc
     4381 cagcctccca ccatctcggc aggagtcgcg gtaaaaccgg acacttcttt ctcgactgct
     4441 atgagggcca atcccttagg gcctaaatcc ttgttcgtct gcacaagaac aaggacgaca
     4501 tctgcaaggt tccctgcggt aatgaaattc ttgcttccgt ctattatcca agtatcaccg
     4561 tcaagagtag cttttgtttc cacagcagag gcatcactgc ctacattcgg ttctactgca
     4621 gccatagcaa caatcagact tccattacag agatctggaa ggtatttctg tttctgctct
     4681 tcgttggcca ctttactcag gatggttcct gcaaggacag cgtggcccag cgaaataagt
     4741 gtttgtgtcc agcttgcccg gctcagttgt tcccatacaa tagatgcagt aacgtagcct
     4801 gcttccatac ccccatactt ttggggtaga tgtaccccga gtaagccctg agacgccatt
     4861 ttttgtataa gagtcaagtt cacttcacgg cggcgttcac agtccagtag tgtgggtagc
     4921 atttcttttt cagcaaaatc atgggccatt ttgcgtacca tagtctgttc ttcattaaga
     4981 tcaaagttca cgtgtgcctc ctttatagag ggatattgcc atgtcgtctg gcgttgcgtg
     5041 taattccttt attttctatt aaattcaggg agcgaaccag gcatcgtcta gtttccgccg
     5101 gttcaataat atcctgaacc cacgtcacat cagaagcaag ggccaaagga tccgcataca
     5161 gatccttgta gtcctgaatt tttcgctcac gatactcctg aggatcttgc gctttcttaa
     5221 tctcatctcc gtagaaaaga tctactgcct gagccgcccc catagcccct acctctgcgg
     5281 ttggccagcc aaagataaga tctgtaccaa accccggcat tatacccata ccgagagcag
     5341 ctccgccata caccttccgt aacagtatgg caatcttggg cacagtggct tcggaaaggg
     5401 catacagaac ctttgctcca tggcggatta tcccggcgtg ctcctgaccc ttcccaggca
     5461 aatatcccgg ggtgtcaaca agaagtacta tagggatatt aaaggcatca cagaaacgga
     5521 tgaaacgggc ctgtttgtca gaactgtcta cactcataca ccctgctttt accataggct
     5581 ggttcgccac aatcccgaca acacgtccat caatacgggc aaaacctacg ataatttctc
     5641 ctgcaaactc ccttttaatt tcaaagaaat caccttcatc gacaatcgtt tcaatcactc
     5701 tgtgcatatc atagccttgc gttggctgtt cgggaacaat ttcctccagc atagctgtca
     5761 ttcgcccggg gtcatcctga gtatcttttc tttcaggccg tgtagtacag ttttggggga
     5821 gataggagag cagttttcta atgctcgcca tgcaatcctc ctcggatttg acgcgaaagt
     5881 cagccacgcc cgttacctga caatggatct tggcaccccc cagctcttca agggtcacat
     5941 cctcatgcat gacagatttt ataacccgtg gaccggttat acacatatga gagagtccat
     6001 caaccatgaa gataaaatct gtgagggcag gcgaataaac agaaccaccc gtgcagttac
     6061 ctagtatggc tgatatttga ggcaccacac ctgagcagcg tgcgttcatg aagaaaacta
     6121 ctcctgcgtg tttctcatcg ctaacacggt agggatcgtc actgcccgcc acctcatgtc
     6181 tgatgacacg ggctcccggc gaatcgttaa ggccgattaa tggcacaccc atatccagag
     6241 ccatctcatg gattttgtag agtttcctcc catgcatgta accttgggac ccacccatga
     6301 ctgttgcatc gtgagcaaaa acgcaaatcc gacggccgtc aatagtccca tgacccacca
     6361 ctatgccgtc acccggtgcg cccatccgat acttaacaag catattgaac tcattgaagc
     6421 tgtccggatc aagaagcaga ttaattctct cccgggcact cagttttcct ttttcgtgct
     6481 ctttagcaat cttttccgca ccaccaccca tggaagctgt ttttttaact tctctaagtc
     6541 gttttatgcg ttctttcatg gttttgctcc tgtcagatgg atttcgcaac aaaaactttt
     6601 gggctgggtt cctccatcaa gatttctcca caaggcttct tgctgaggtt tgcgttcacc
     6661 cttatcatct ctcctgccgg tttctcggcc ccagttctaa acatttcatc ctcatcaggt
     6721 actaaaagtt gcctctcctg cgtggggaac tgaaaaatcc ttttttcggc gtatatggat
     6781 tttttcttat ctttctccat actcaacccg caagaagtta ctgttcaggc ggtaggtacc
     6841 aggccttccc ctttagcctg attctcgtcc tcaccccttc cggctaagga tactgaagga
     6901 taagaacaca tttgactatc cttccctgta ggtccagctc atatcatatt caggccacac
     6961 agggtcgaca cttaaaatcc tgtattttag agaatcatat aattcgtatg atattttgtc
     7021 aatgtaaagg tacacccaaa atctattttt gtgtttcacc atcctcgaac gtattgatgt
     7081 gcttaacttt ccttttcgaa aaatctgaaa gatcacatga aaaaaggggt tattgaacat
     7141 aatcaccaca tgtatatttt cgacaaaggg tgttctctca ccttgctgtg cagtataaag
     7201 tgaatggctt tggaaagttt acaattttaa ttggatgatt atctttttca tacagataaa
     7261 aaataaaggt gatctttgaa aaggataaca acaattctaa ttctgttagc agcgacattg
     7321 ggcttcaacc agataagaga ttttggttat acaatggagg gaaaaatggg agaagtattt
     7381 caaaatacaa aatcagcagt ttttgctggt gggtgtttct ggtgcactga atcagatttc
     7441 gaaaaagtcg aaggtgttat cgacgttata tcaggataca ctggaggcac tatgccgaat
     7501 ccaacctatg aacaggtttg cacgggtaat acaggacatc tggaggcagt caaagtcatt
     7561 tatgaccctt caaaaattac atatgaggaa ttactcgaca tattctggcg acacatagat
     7621 ccaacagatg cgggcggtca gtttactgac agaggaaccc agtatggcag cgcagttttt
     7681 tacacaaccg aagaagaacg caaatctgct gaaaactcga agaaaaacct ggctgccatg
     7741 gggcctttta cagaagagat tcgtactgag atcctccagc tcggcccctt ctatcccgct
     7801 gagccctatc atcaaaatta ttacaaaaag aacccgagcc attacaacgc ataccgttct
     7861 ggatctggtc gcgaccggtt tcttaaggag gtttgggcag agaggaagag cacagagagt
     7921 tctggaataa agaaggaaac aaaaaagaat gtggcaacaa ctgaaaagat tgcttcacct
     7981 tatttaatcc ccagtgacac ggaactgcaa aagcgcttga caccactaca gtaccacgtc
     8041 acccgacagg atgggactga gccccctttt aataatgaat actgggacaa tcacaacgaa
     8101 ggcatttacg ttgacataat atcaggggaa ccactgttca gttccaccga caagtttggc
     8161 tctggaaccg gctggccaag ttttacccaa cctctggagc cggaaaatat tgttaaaaaa
     8221 accgaccgtg gttttcttat ggtacgcacc gaagtacgca gcaaacacgc aaactcacat
     8281 cttggacacg tatttgacga cgggccaaag ccaaaagggc tgcgttactg tattaactcg
     8341 gcggctctgc ggtttatccc caaggctgct ctggaaaaag aaggttacgg taaatatcag
     8401 tatcttttca gccggtaaaa ggacctatac cacaaattct atttcaaaca ttgtcatttg
     8461 gggcacattt ttccacttac agtgatgtag actgaaatga tgtgaaaaaa aagggggctg
     8521 caacaattgc agccccctgt gctcaactac aaacagacgg acaggatcct gtttaaacgt
     8581 atcatgctgt caggcatata cttactgcct catccatcct ctcgatgaac ttgaactcca
     8641 tgttctcctt tatgctctca gggacctctt caagatcctt ctcgttcagt ttgggcagga
     8701 taacgctttt gatgccagca cgttttgccg caagaacctt ctccttgatg ccgccaacag
     8761 gcagtacaag gccacgcaag gtaatctctc cagtcatggc tacatcattt ctcacatgcc
     8821 tgtctgtcag gagagacacc agagaaacca gcatggtgac tccagcagaa ggtccatcct
     8881 tgggaatagc cccctgaggc acatggacat gtagatcatt cttttcaaaa aagtcttcgg
     8941 taatattgaa atcagccgcc ttaccgcgga tatagctcag ggctgcctgg gctgattctt
     9001 tcataacatc accaagctga ccggtgagtg tcaggtttcc ttttcccctc atcttggttg
     9061 attcaatgaa cagtatatcc cctcctgttg gtgtccaggc caggcccgtt gccacacctg
     9121 aatatttcac cctttccgct acttcagaaa agaattttat gggcccgagg taaccgtgaa
     9181 tactgtctgc tgtaattatt tttttttcag ttgtgccctc ggcaacatct ctggccactg
     9241 ccctgcaaat tgtggcaatc tccctttcaa gatttcttac accggattcc tttgtgtatg
     9301 atttaatgac aaccttcaat gcatcatcct gaaactcaat aagatcttca gtcaaaccat
     9361 gctcgttacg ttcctttggt ataagaaact gttttgcgat cataagcttt tcttcttcgg
     9421 tatatccggg aagttcaagc acttccatcc tatccttaag cgcgggaggg atagggtcaa
     9481 gcatattggc agttgctata aacatcactt tggagaggtc gaagggcact tccagatagt
     9541 ggtcactgaa agaaaagttc tgttctgggt caagcacctc aagaagcgcg cttgacgggt
     9601 cacctctgaa atccatgcca atcttgtcca cctcatcaag catgaaaaca ggattgttcg
     9661 agcctgcttt cttcacaccc tgaataattc tacccggtag ggcaccaaca tatgtccttc
     9721 tatgacctct gatctcagct tcatctctga ttccacccag ggaaatcctc ataaattttc
     9781 tacccagagc cctggctatt gactttccga gagatgtctt tcccacgccg ggaggaccag
     9841 caaaacagag tattgggcct ttcatatccg ctttcaactt tctaacagcc agatactcca
     9901 gtatcctttt tttcactttt gtcaagtcgt agtggtcttc gtcgagaatc ttgctcgcat
     9961 cttgtatgtt caagttgtct tcagtcgcct gcgaccaagg aatctctgtg agccagtcga
    10021 gataagtcct cgatacggta tattcagctg acatgttgct catcttggaa agtcggtcaa
    10081 gttctttctc tgcaatcttc tggacatcag gcggcatcct cgcttcaaga atcttctttc
    10141 taatctcctc catctcagac atcttgtcgt cagtttcacc aagctctttc tggatggcct
    10201 tgagctgttc ccgtaaatag tattctctct gggtcttgtc aattccttct ttcacatggg
    10261 actgaatacg gctgctcagt tcaagagtct ccacctccct gttcaacagt atggtaactt
    10321 tcttgagcct ctctttcaga tcgatttttt caagaatctc ctgcttttct gcaactccga
    10381 tgttgattgt tgatccaatg aggtcgacca ggttccccgg gttctccatc tgacttgcaa
    10441 tctgcgaaag ctctgatgag agatagggcg ccatttcaac cgctttcttg tacaggtttt
    10501 tgagattgag gtacatggcg tcaatctcga tatctttctg atattcctca aaaattggca
    10561 ggatcttggc acgaagatga ggctccgtac ttgtgaattc caccagtttg aacctgcaaa
    10621 ttccctgaac tacaattctc tggctgccat caaccatctt caccatcttc atgatggttg
    10681 caaccgttcc aatcatataa agatcatcta tccctggatc ctctatgtca ggatttttct
    10741 gtgttatgat cccgatcatt ttgtcaccgg cgtttgcctc atcaacaagc tttatggaac
    10801 gatcgcgccc cacaatcaga ggcataacca gatcagggta cgcaaccgtc ccacgcaatg
    10861 gaagtatagg caactcggcc ggtataaata ttttatcttt tagagtcttg tcattcctaa
    10921 aacctattac cattcattcc ccctatttct taacatcctc gaattctgcg tccaccacat
    10981 cgtcatctcg tctgcctcca ccggcctcac tctgtgtctg ggccccctct ggtccagggc
    11041 cttttgatgt ggtttcagaa gtcctcttgt atagcgtctc tgcaagttta tgggaggctt
    11101 ttgtcagctc atcaactgca ctcttaatcc tgtcggcatc tccggtcttg acagtttcct
    11161 ttgccctggt gagaacgtct tccatagacc taatatcgtc agcagacagt ttatctttac
    11221 tttcgttgag tgttttttct gtggtataaa tcatattgtc gagctgattt tttatctcga
    11281 tttcatgttt tctcttttta tcttcttctg agtgtgtctc agcatcatgc accatcttct
    11341 taatctcatc ttcgcttaaa cctgtggaag cagtgattgt aatattctgt tcctttcctg
    11401 ttgcaaggtc ttttgctgaa acatgaagga tgccgttggc atcgatgtca aatgtaacct
    11461 ctatttgggg tatgcctctt ggtgctggcg ggatacctat gaggtgaaac tttccaagtg
    11521 tcctgttgtc ccgggccatc tcacgctcac cctggagcac gtggatctca acacttgtct
    11581 gactatcttc agctgtcgta aaaacttgac tcttcttggt tggtattgtg gtatttcttt
    11641 ctataattct tgtcatcaca ccgccgagag tctctatacc gagtgaaagg ggcgtaacat
    11701 cgagcaacaa cacgtctttg acctcgccag ccaagacgcc tgcctgaact gccgctccaa
    11761 gggcaactac ctcatcaggg ttaacccctc tatggggttc tttaccaaaa aagtttttaa
    11821 caacttcctg taccttggga atccttgtag aaccacccac caggacaacc tcatcgattt
    11881 tgccagggct cagcttggca tcttccaatg ctcgtttaca gggctctacg gtcctttgaa
    11941 taatgtcatc acacatcttt tcaagttcag cccttctcag tttcagattg aggtgcttgg
    12001 ggcccgatgc atctgccgtg ataaacggca ggttaatctc cgtttcaatt gctgttgaga
    12061 gctcgatctt cgccctttca cctgcttctt tcagcctctg aagagccatt ttgtcctgtg
    12121 ccaggtcaat gccctgatcc ttcttgaatt cggctactat ccattcaata accctctggt
    12181 cgatatcgtc accgcccaaa tgagtatctc catttgttga ttttacctcc acaacgttat
    12241 caccgacctc gagaattgag atatcaaagg tacctccacc aaaatcgtaa accgctatcg
    12301 tctcatcctt tttcttatcc agtccgtaag caagggctga agctgttggc tcattgatga
    12361 tgcggagcac gtttaagcct gcgatccttc cagcatcttt tgttgcctgc ctttgggagt
    12421 cattgaagta agctggaacc gttataacag catcttctat agtctgaccc agataggctt
    12481 ctgcagactt tttcagtttc tgaagaacaa aggctgatat ctcctgcggc gtatactgct
    12541 tacccctaac ttccacgcga gccaaacctt ccgaatcgct gacaaccttg aaaggaacgg
    12601 ttttgatttc actctgtacc tcgttatatc tcctgccaac gaaccttttt atggaaaaaa
    12661 ttgtattttc agggtttgtg attgcctgcc ttttggctgt ttgcccgaca agtatttccc
    12721 cgtcttttgt aaaagcaaca acgcttggtg taagtctatt accctcttcg ttgatgatta
    12781 cctttggttc acccccttcc ataatagcca cgacagaatt ggtagtacca aggtctattc
    12841 ctataacttt ttttgccata tatgtatcct cctgattgaa tttagcttac ttaagtgaga
    12901 tgcttaaaat tcatacgtaa tataatctcc gagggtatcc ttgtcaatat atgatggcat
    12961 tgcaaaagat gactctcggc cttatatttt caagacgttt tcgtttgctg aaaacctttc
    13021 tatcttgtgc accttttatt taacaagtaa ccggccatag acaaaggaac atacaaaaag
    13081 caagaaattg agtaaaacga tcgttgcacc tgaaggaagg tcaaacacaa gggaaaaaaa
    13141 tatccccaaa ataatagata aaacaccgct cactgaagca ataacaatag tcatcttgaa
    13201 gcctcttgct atctggagtg cggttacagg cggtagtatc agaagggacg atatgagcat
    13261 aatcccaacc acccgcatag caaggactac tgttagtgat gtcaatagga caaggattcc
    13321 attaatagtc ttggtcttta tcccactcgc tcttgcagac tcttcatcga aagtaattga
    13381 caaaagctca tgataataat accagataac tgacatcaaa atcagggata ggaaaattga
    13441 agaaatgacc tcggcattgc tgatagccag gatattgcca aaaagatagc tgaaaaggtc
    13501 tatattgaag ccaccggaaa cacttgccag gagaataccg cacgcaatac ccagagaaga
    13561 aacgatacca attgccgcat caccatatac tcttgctttt tcaacaagtt tgaggatgcc
    13621 aagggaactg atcatggcaa ccggaactgc agcataaatt gggtaaacat tcaagaacag
    13681 accaactgcc acaccaccaa aggtcacgtg ggcaagacca tcgccaatca gcgagaaacg
    13741 gcgcaacaca agaaatgttc ccaaggttgc gcagagtatg gcaatgaatg atcccgccat
    13801 gagcgctctc tgtataaatc catgactcag taaatttata atatccataa tgaaattatt
    13861 aggcaaacag gctttcagta attggtggcg agtaaacgta cataaacttt tctatccctt
    13921 ttccgtaatc ctgtttcttt cctcttaccc ctaacctctc aacctttttt tgcatggcgc
    13981 ataaccatgc atggccttca atcatgtcta tggcatataa tatgctgtga atattcacca
    14041 aaataacttg ccatatcatt ggattcacaa aaatcatcaa aaccaccata gaaaacaacc
    14101 tgtttatcca gataaagaag ccgtgaagca tactttccaa tagtgccaat atcatgggtg
    14161 atgataatta ctgtcacttt tttgtgttca ttcaggtctt tcagggtact gaaaaacttc
    14221 tcccgtgctt cagggtcaag ggcggtggtc ggttcatcaa gaatgagtaa ggccggctca
    14281 ttaataagtg cccttgcaat gagaactcgc tgttgttggc cccctgaaag ctcaccgatc
    14341 aatttgtttt taatatccag aatttcagtc aattccagga cattattgat ggctttttca
    14401 tcatttttcg tgatgcgcct gggattgctt ttggttgaga gcagaccaag ggcaacaacc
    14461 tctctcaccg tagcaggaaa atggggatta aaagaattga tcctctgagg aagatagcca
    14521 atcttatgcc aatcaacaaa atcagccgtt ttggttccaa atatcttaat gactcccttg
    14581 cttggcagta gaagaccaag ggtcagctta atcagggttg acttccccga gccatttggc
    14641 ccaacaatac caagataatc tcctacctgc agatgaaacg atatatctga aagtatttca
    14701 gttgcattgt actgaaaaga aaggtcatgc actgatagtg catctattgt tgacattcaa
    14761 ggcctacctt caggtttttc aggttttctt ccatgatact gagaaaagaa acgccttttt
    14821 caaaatcctc ttttgagata ttgtgagctc cgtgaagtct gagaagcctt gctccggttt
    14881 cttttgccag aatatttgcc attctcgggg caataagctc ttcatagtag acatactgga
    14941 tatggttttc tcgtattttc ttttttagag aaattatctg ccacggtgat ggttcagtgt
    15001 caggagagcc cgggtaggca gaaatatacc tgaggttgta tcgctttgca agatagttaa
    15061 aggcaaagtg gccaccatgg acaaaaatgt tttttttaca ggcaaaaaga cctttcttgt
    15121 accgcgcgtc aagatcagca agcttcatga tataagcatc tgcgtttttc ctgtaaaagt
    15181 ccttgttatc cggatccacc gttaagagag ctcccagaat attggcaacc attttctggg
    15241 cattggcaag atcgagccat atatgaggat caatctttcc atgggcatgg ctgccacctg
    15301 tgcgatcttt ctcccctttc tcgtctttat ggtcgccatg ttcttgtttg ccttcctcaa
    15361 gggttatacc tttgctcgca tcaaccacaa cgagttccct cttgtcaacc cctctgagaa
    15421 gaccataagc ccagggctcc atgtattggc cggtaaacac aaagacatca gcgttgttta
    15481 ccttgagcac atctccagcc tttggttcaa aactgtgagc ctctacccca ggaggcatga
    15541 gaagtgtcac ccttgctttg ctaccggcaa tattttttgt gaaatcgtag agcggaaaca
    15601 aagtggttac cacgctcagt ttctttgcct ccactgcctc ttgctcccta tgctgacatc
    15661 cagcccctac taaaaaaagg atgaggctaa aaatgaggat agaggaaggg acagcattag
    15721 tagtcataca aacaccctat gttgggaccg catgagaaag agtacaatcg ctgcacaaac
    15781 catagacttg aaaaaggtga gaaaccactt tccccttcaa cgtatcttcc acttccctga
    15841 ttattttctc ttcaccgcaa aactcgaggt cctggaccct gcgacaggct atacagacaa
    15901 aatggtgatg atggctggta ttattacaat agtaatagta aagtttacgg ttagggtgga
    15961 taatctttat aataactccg ccattcgaaa gttcttccaa gtttctgtaa acggttggga
    16021 gtccgattct tttgaaacgt gtctgaagtt tcctccaaat ctcctcagga ccaagatagc
    16081 aaaatgcttc ggcaagaatt tcaaggattg caatcctttt gggtgttgcc ttgagaccaa
    16141 gttcctttac tatacgattg gtatccattg ggtcatgcct gtactaaata gaattaattt
    16201 ctaattagaa agtctacccc tatggcataa accagtcaag aataaaatct gaaccaggct
    16261 ttttatattt taatagcgta tctactggcg ttgccattcc ctaaaaaacc gttcactggc
    16321 tcaagcggtg acttcgtcca cactcagctt cggaactttt gcttatacaa atgaccaaat
    16381 gtccatgctt cgtggcagct tgtgtcagaa aaacctgaca ccatatctgt agaaaataag
    16441 atcagcatta tattgtaaat tgtttaaatg agggtgtggt ctctctatcg ctctactggg
    16501 taccctttgc gcatccattc aggaaatcct gccctaaaag taaagacttt ggtataccca
    16561 aggtcttttg ccgcgaccgc agctttgacg cttggatatc acccggggcc cctgcagtaa
    16621 aatacaagaa gagtctcttt tgccggtgga agaatcagtt tcagtgcata aaacttttct
    16681 tcgggtacaa agagtgatcg gggaatacgg cctcgtgcat attcaatttg cgttcttgta
    16741 tcgatgacca tgagattggt ctccccattc atcattctca tcaattcatc agcagaaaga
    16801 tcaggtatat ccggtttgat gccacaataa aataagaata gagcagatgc aaagatcaga
    16861 ggcaatgtaa ggcgtctcaa tctcatgcta ccttttaaat atctccttga tccttgcctt
    16921 tgtctcgtcg atctgtgctt gagaaagctt tagtccacca tgctcctgtt ccggtgtgct
    16981 ttctattgaa taaattcgtt cttccatggt agcctgcctc tgcataccag gaggaagatc
    17041 cggctcaaca ccaatggctc gctcaacatc ttcaggaaat tttcctggat cagctgtttc
    17101 atatacgact gcaggttcat cgtgacggcc tcccaggcat acctccaaga ccttccaacc
    17161 taccgcacca tggggatcaa ggacaactcc gtatttctca tacacttctt tcatcgtttc
    17221 aaaatgttgg ggatttgtaa cacccatgga tacaatatct tttctcattt cgtccatgtc
    17281 aggcattcgg tcaataacac ctggcttgat aatctttccg gttactctat cccgttcatc
    17341 atacatgtga ccgccataaa aatcaatcag gcgggcgagg ttgctgggat gagaaacaat
    17401 catggctgaa gaaggagact ttatggaagg tctcacctcg tattgtcctg tctccagaaa
    17461 ctcgggaaac tcagtgtttt cattaactcc tgctataatc ttcgagacag gaagccccat
    17521 tcctttggca aggacagttc ccatcatgtc tccaaagttt ccagagggga tactggcaat
    17581 tattggctcc atattatctg ctattcttga ataggcataa aacggatata ctacctgagg
    17641 aaggagacgg ccgaggctga tggagttggc cgatgtaaac cgttccctgt cgttgaaaag
    17701 ttcctcggca aaggactgat caccgagaat cgtttttgca agggcttgac acacatcaaa
    17761 gtctccgttt atctcaaagg catatatgtt gttcttgagg gtcgtcatct gccttctctg
    17821 accttcactg atagaacctt tcgggaaaaa gacaatatta tcaatattct tcaacccaag
    17881 aagggcatcg gctactgctc cgcctgtgtc gccgctggtt gccaccacga ccacccttcg
    17941 gagtcgcctc accccaagga agtaattgag catgcgaccg aaaaagcggg ctgcataatc
    18001 cttgaacgag taggtaggtc ctttcgtcag ccacatgata tgggttcgtc ccgttacttg
    18061 ctggatatct gttgggatga catcttcgcg ataggcgtcg ttcaggagtg accgtaagac
    18121 cttttcaggc acctctgatc tgagaaaagg agaaagaacc tcgaaggcaa tctgcggata
    18181 atacatgaat ggcatggcca tcagcttctc gtgggatatt ctgggaagct ctttccttgg
    18241 taccatgtaa aggccatagt ccggtgccat tcctctcaag agggcatctt cgaaattgat
    18301 ccgttcattg ggattgtttg tgctatagta agttattggt tccacgggtt aataagcata
    18361 caggatacag ctcagttttt caatgggaac agattaccta ttgaatcagg ctattccact
    18421 gaggtgtgca ttggattcgc acattcttcg cagacacgac atcacgcctc gatagcattc
    18481 gtggaataac aatccatatt gcaatgggac atttttttct tctcaacgca aaactcaaag
    18541 ttgctttaca ttcagccatt ttttgctgaa atatcgcctg gaaataagaa aggccatgac
    18601 aagctgggat atattcatga cccaccacac tgaaattgcc ccgaaaccaa ggattacaac
    18661 aaagagatag ctcagcggca ccctgacaag ccatattgac attcctatga gcaccatgac
    18721 cgatcttgta tctccagcgc ccataaggcc accacccaga ataatggccc acgccataaa
    18781 aggctcactt aacatattga tgtaaagata tcgtattgtc tctgcaacga caatcccatt
    18841 gttcgaaaga attggcgcta tccatcgggc attcaggatg actgccagtg tcagggcaat
    18901 gacaataggc acaccgagac aggctgttat gatgccaccc ctaaaggcgt cttcggtctt
    18961 cttttcaccc agaaaattac cgatgactac tgcatttgcc atattaaaag caaaagcagg
    19021 caggaaaata gctgactcaa tccgtatacc tgcagacagg gctgcaagta tttctacatt
    19081 attcttggga agtgagctta aaatcagaaa aattgccatt gagtgggcct gccagagtac
    19141 ctgtgacagg cctgatggcc agccgatgtt catgacactt tttgaaacag cccgggagaa
    19201 ccgctttatt ccatccatga gattccatac atggaagctg tttataaaac tccctataca
    19261 gacacttacg gcagtggcca cggcaatccc cttataacca agagacgtgt taaagacaag
    19321 caagaaagca aggctgatat tacatgcaca gacaatgacc attgttttca ttgatgtctt
    19381 ggccatttta cacgccctga gtatcccatt ggtctgtatc aatacgtagt ggaacgggag
    19441 accggctgag tagataaccc caaggggcat tgccagaggc tttacttctt gtggtatatt
    19501 tagcagggca attatattgc cagtcagaaa aaaaccccct atgcccagaa atacccccgc
    19561 tataatagtg atcgacaatg tcgaataaat cgcttcagtt atttcatctt tgtttccggc
    19621 agtaaaaagc cttgaaataa tcgacactgc acccacagtc aatgcatttc ctacaatgat
    19681 aaagacaaaa tagagttgaa taatgaaacc gtatgcagcc tgtatctctt tgccaatctg
    19741 tcctgcaata taaatgtcaa caagacctgt cagaaattca aagaacatga tgaggatcat
    19801 aggccagctg atagaccaac tgtcccggag aaagattttt atctcattct tttcaataat
    19861 cttgggtaga ttcatagtcc ttatgcagta aggccacacg cacttatatg agagcatgtt
    19921 tgaaaactgg acgtcgggtc tgtctttttt cgtaacttaa acctttggca gccctgaaca
    19981 gcgagcctta agatcctttt ttaaaatttt tatatgattt cccttgacaa aacaactgat
    20041 ttcttttaaa ttcattcacc atgaataatc gattaccttt ctatggccat ttttattttt
    20101 actttaggaa cttgcaccca tgtagtattt tgctgtgaaa aagcaaaagc catgggtgca
    20161 caacccatgg cttttttttg tgtaaagcca tggtaacaac catggctttt ttattatgag
    20221 gaggacaaaa tgattatttc taatgagatg gatcaattaa tcaaagacca ggaaggctgg
    20281 acccggagga tgtttgaaga aggaatcagg atcaagaaac tgtacggcct ggatgccgtt
    20341 tgtgattttt cccttggaaa tcccgatact gagcctccgg aaaccctgag gaaagcactt
    20401 atcgatatgc tttcaagccc ggaagttggt acccatagat atatgtctaa caatggatat
    20461 gaagatgtac gagaaaatat tgctgagtac ttgcgcgaag tgcatggtgt tccttttact
    20521 gcaggtcaca tctttatgac tgttgggtgt gcaggcggtt taaacgtgat cctcaagagt
    20581 atgctgaatc ctggtgatga agtcatcgtc cctaccccat ttttctggga gttcaaaaac
    20641 tacataaata attttagtgg agtgacccgc tttgtggaaa caaaagaaga ttttcagctc
    20701 gacattggaa atatagaggc agcaatcaca aaaaaaacca gagcgatcct catcaacagt
    20761 ccgaacaatc ctaccggtgc tgtttaccat gaagagagta tgaaacagct tgccagcctt
    20821 ttaaacacaa aacgggaact gggccaggat atttacatca ttaccgatga tgcgtacaaa
    20881 aaacttgttt acgatggtat tcagattcct aatattttta agatgtacga tctggttctt
    20941 tctgtgacct cccattcaaa ggaccttgcc ctggccggtg aaagaatcgg atacgttgct
    21001 atatctccga gcatcaagga tatggacctt ctcgtctctg ccatgacaat ttcaatcagg
    21061 gtccttggtt ttgtcaatgc acctgccctt tttcaacggg tggcaggaaa gtttcagagg
    21121 acctcagtca gtatcgccga ttacgagaaa aaaagggatg tcctctatgc aatcctcatg
    21181 gaggcaggtt ttgaatgcgt aaagcctcag ggtgcttttt acatgtttcc aaaatcgcct
    21241 atacctgatg aacttgagtt tgttcgcacc ctccagcaag aagagcgtat catggttgtt
    21301 cctggcagag gattcggcag ggaaggttat ttccggatag cttactgtgt acctatggaa
    21361 aaaatactca aggcccgtga aggcttcttg aggacaggcg atcgatatta tagaggaggg
    21421 aaatgatgcc gatttcaaag aatatctcaa cacttatgaa aaactcttcc tggattcggg
    21481 ccatgttcga ggagggagaa gaattaaaaa agaaattcgg gactgataat gtgtgtgact
    21541 tttccctcgg caatcctata accgaacctc ctgaagcttt aaaaaatgag ctaatcaagg
    21601 ttgcttcatc cgatgcacag ggcatgcatc gttatatgac aaatagcgga tacgataatg
    21661 taagggaaga ggttgcccag tatcaaagag aaataacagg acttccctat acgagggatc
    21721 acattgtaat gacagttggc gctgcaggcg gtcttaacgt gatctttaag tcaatccttg
    21781 atccagggga cgaagtcatc gtaccatctc cattctttgt ggaatttatg ttttatatcg
    21841 ggaaccataa cggcatcata aaacttgtcg acaccaagga tgattttcat cttgatattg
    21901 ataagattaa ggctgccata agcagaaaaa ccaaggcaat ccttattaat tctcctcaca
    21961 atccgacagg cgttgtctac aatgaagcag agcttttgga acttgcagat cttcttcacg
    22021 agaggagcaa aaagggccag cgaatttata tagtcgctga cgaggcctat aaaaagatta
    22081 tttataatgg aatcaaactg ccgaatctgc cgaatatgta tgaagatact gtaaatgtga
    22141 catgccactc gaaggacctt ggcctcgccg gggaacggat tggatatatc gccatatcgc
    22201 cacgcattaa aaatgtacgc tcccttatcg atgccgcaat ctttgcaaac agaattctcg
    22261 gcttcatcaa cgcaccggca ttaatgcaaa gggttgttgc aaaatttcag cacaatagcg
    22321 tagacataat ggaataccag aaaaagagag acgctatcta caatattctt gtggaatcag
    22381 gctttgaggt cataaaacca gagggagctt tctatatctt tcccaaatca ccaatccctg
    22441 atgatattca attcgtgaaa accatgttgc agcaccgcat cctcacagtg cccggggttg
    22501 gctttggaaa atctggatat ttccgtttag cctactgtgt tactatggat ataatagaaa
    22561 agtcgaggcc gcacttcaag gaagtaggag agctgttgag aaagggacag aaataaaaca
    22621 gtgatccgtg acgcgtgatc ggtaactttc agaataggat gaaagaaggg cgcaggcgct
    22681 ataatcattg ccttttgcga acaagcgagg tgaacgtctt agcgaagcgg tcatccttcg
    22741 aatattcgga aaaaaaggag acgacgtaag tcccagttat tgctctaaaa gtggatcggt
    22801 attgatgttc tttgcgagaa tatctgccat acgctccctg tccacgtttc ctttaatcaa
    22861 gtccttcacg tgggggaaga tgccaaggat gggaatatca gccagatatt tcttcaaaat
    22921 ggttggattt gtctgcgttg gtaaatcatc cgaaccagcg ttatcattca gaatcacccc
    22981 aagaacctta attccccgct ccttgagaag atgaatggtc agaagtgtat gattaatcgt
    23041 gccaaggcca agccttgaaa cgaccagagt tggcgcattc catttctgga tcaggtctat
    23101 aaagaaaaaa tgctccctta taggtacaag aacacctcct gcaccctcta taacaagcaa
    23161 gtcatgtgac ctcttaaggc cttggaaaat cttatctacc agactaatat ctatgctcac
    23221 gtgttctatc atagctgcaa cctcgggtgc gcacggagct tccagcgtat aggggcttat
    23281 gatgttgagg ccatcggtgc ttcctgatgc ctctcgcaaa cagattgcat cccagggcag
    23341 aagatctttg tcagtctttg agagtccgca ttcaaatggt ttcatgactc ccacattcat
    23401 tttttttcta agggaaaaaa atgcagaaac tgctgaggat ataacagtct tgcctattcc
    23461 tgtgtctgtt cctgtgacaa aaattacttt cattaccgta ttattgaagc aagtaatagt
    23521 gacaatccga ttaaaagacc tgcgcacaga atagaaagtg ccatattccc atcttcacta
    23581 atcttttttt cgaattctga agtgaaggtt gctccgaaga ccttcagcat tatcaggaca
    23641 ataattatgt agaccgccga gtagacaaac atctcgatga tattactcag cactctccac
    23701 atgataacct cttaattgga caaataatag acaagattgt ccagtgcagc ggtaaaaaat
    23761 atgtttctta acgggatgtt tccatgtgac ctgacaggca agggggcaaa attgagaatt
    23821 cccttaattt cagtctctga tagcaggtca gctatttgtt gcgctgcccc gggaggggtg
    23881 gtgataatgc ctatttcaat attccttgct ttaatgactt ctttgagcat atccacatgg
    23941 agaatctcca cgggttttcc catcctctcc gagatacggc caatcacgct tactgggtca
    24001 atatcaaagg ctgccacaat cttataattc tgcttgagga aatctttgta gacaaggagc
    24061 gcgcttccca tgtttccaat gccaataact gctatacgcc attcccggtt aacccccagg
    24121 atttctttga tatggaattt aagctccttg acattgtaac ccattccacg aataccgaac
    24181 tctccaaagt acgcgaaatc ctttcttacc tgggccgcat ttacattgca aatctcagca
    24241 agtaaagcac tggaagcaac tttttcattt ttagactcga gatcctcaag ggccttaaga
    24301 tagttcgaaa gtcttctgat tgtcgccccc ggaatcttag ggtatttcga cattttatgg
    24361 accttttcat tcataccgtg cgagttttta tttgtaacaa ctattcgatc gaaatgcaag
    24421 agaaaatttt ctcaagcaga ttaagagtta taaagaaata ttatcttttc attgatcttt
    24481 agagtcccag cacatccttc attgtgtaca caccgggctt ctgtttaacc agccatcggg
    24541 cagcaacaag ggcacccctt gcaaagtttt cgcgggaaaa cgccctgtga gtaatctcga
    24601 gacgctcccc aataccggca aacataactg tatgttcacc gacaacatcg ccacccctca
    24661 ggctcatgat gccaagctct tcctttttcc gctctcccag cattccctgt ctgccaaaaa
    24721 cctcaatcca ttttctgtca ggtgcagcat ttttgataag atccttgagt ttaaccgctg
    24781 ttccgctcgg tgcatccttt ttcagtttat ggtgcatctc aaaaatctct atgtcgtaat
    24841 ggtccttcat ttttttggat aatttctcaa ccacatcgaa catgagattc atacccacac
    24901 tcatgtttgg tgatataaca atacgagtgt ttttcgtctt cattatttca tccaagccct
    24961 ggcccttcag ccctgtcgta ccaatgacaa tagcctttgc ctttttcttg accagtctga
    25021 agttttccag ggtagcctcg ggctctgtaa agtcgattac cacatcaccc ctgtcaataa
    25081 cactggcaag gctgccttca acgatgagag cattaccggc accgacaata accgcttctg
    25141 cacacttgcc aacactggga tggtgctcta tttcggttac gccgctcacc atgaaatctg
    25201 gatcctccag agcaagtttg aggatcatac ttcccatcct cccgcaaaca ccagtaatta
    25261 ttgttttaat catacacacc tcaaaccatt ctatagcagt tctattctct tttcagaagt
    25321 ccgtattccc tgagcacccc tttcagaaat ttactgcttc cttcagacag gggacacagg
    25381 ggcagtctga actccttttc catcattccc atatgataca aggtctcttt gataggaatg
    25441 ggattcgttt ctacaaacat agcctgcata ataggtaaca gacttgtatt catgtccatt
    25501 gccttttgca tgtctttttc tccaaggaac gcctgataaa gggcctggag ttcaactggc
    25561 atggcattcg agagaacaga tatcactcct ctggcgccta cgctcatcat aggaagaaaa
    25621 aggttgtcat ccccagacat gatggtaatc cgttccttgg caagcctata tatttctgct
    25681 acttgaacaa gcgatcccga cgcctccttt atccctacaa tattcggtat ttgggcaagt
    25741 ctcgctatgg tatcgggatt catattgatg ccagttctac ccggtatatt gtacagaatg
    25801 atggggatat ccacctcttc agccaccttc ttgtagtgct ggtaaaggcc ttcctgggtt
    25861 ggtttgttat agtaaggtgt tgtaaggaga cacatgtcag ctccaacatt ttttgcactc
    25921 tgtgacagtt ctattgtctc ttttgtgctg ttcgaacctg tgccggcaac caccgggaca
    25981 gtacctttta cgtgttttac agtaagttcc acaacctttt catgctcctc ataggaaagg
    26041 gttgctgcct cccctgttgt gccacatggc acaagcccat ctaccttgct cttaagttga
    26101 aaatcgatga gtctttcaag tgcttcttca tccacatcgt agttcttaaa tggtgtgacc
    26161 aatgctgtat atataccttt tagttccatt tcctctcctt ctttacatca atgcttcttc
    26221 atggagtttg cccacgtata tgatctttgt aacaccttct aaatagacct cttcgtctat
    26281 gtaaactttg agtgtttccc cacccctggt caaaatgttc atgggactct tcagtaaacc
    26341 cttggcttta aggataacgc ctgcagcaac agcaccggtt ccacaggcat aggtttcagc
    26401 ctctacaccc ctttcatacg ttctgatttt cacgtttttt ttatcaacaa tctgaacaaa
    26461 atcgacattt gtcccctttt ctccgaaagc tttatggtat cgaacaagcc tgcccaattc
    26521 ctctaccggc atgtggtcaa catcatcggc aagaagaaca gcatgaggaa cgcctgtatt
    26581 tacactactt aaaaagatct ccttgtcttc caggccaaca gggtaatcca acttaatatc
    26641 cgtaggagac gtaagctgga gtttaactct tgcatcatca acttcagcct ttatgatgcc
    26701 tgcaatcgtt tcaaaactca tcttctcttt ggctattcca tttatgtatg cgaaccttgc
    26761 tgcacaccgc ccaccatttc cgcacatctc agcctctgac ccatcagcat tgaaaaacct
    26821 ccaccggaag tcacacatgg ccgacttctc gataaggata aggccgtcag atcccaccga
    26881 gtgatttctt ctgcaaacct taacaacaaa ctcttttggt tctttgaagt tatcgtaaac
    26941 tctgccatct ctgttgtcta tgataataaa atcattgcca ctcgcgctca ttttgaaaaa
    27001 ttgtaggtct aacacgtgtt cattcctcca aaaatgatgg gacagactct ccgcgaatga
    27061 ggtctttgaa gacctcgcgc ttcctgatga tgaaaaattc cttaccctta accagaacct
    27121 ctgccgctct tcttcgtgaa ttatagttag aagacatgga aaaaccgtat gcgccagcac
    27181 cctttattgc gagaagctct ccactttcaa ggtcagcaat ttgcctgctt ttcgcaaaaa
    27241 aatctccaga ttcacaaata gggcccacaa tatcagcctt gatctctcta ctggttttcc
    27301 tgcttacagg gacaatttca tgatatgcat catagaaggc aggcctcacc aggtcattca
    27361 tggctgcgtc aactatataa aaggtttttc ctggaactgt ttttttgtac agaagctttg
    27421 ttacgaggat accactgttc cccacaagaa cccttcccgg ttcaaggatt atcgtaagat
    27481 ccgctccttc caactcctgt ccaatggctt tgccatattc gcctgggtga gggggaagtt
    27541 cgtctttata ggtgatgccg agaccgccac caatatccat atacgcgaca gggataccat
    27601 cccccctgag ttgcataacc atttttttca gtgatcgcac agcctcaaca aatgggccga
    27661 gctcgagtat ctgtgagcct atatgggatg atatccctat tggatcaagg tatggatcac
    27721 ttttcatctc atgataaagc ctgtatgctt caccccataa aacgccaaat ttattcttct
    27781 tcagtcctgt tgtgatatag ggatgggttt ttgcgtcaat ctgcgggttt accctgacag
    27841 aaagagggat tttctttcgc atattttttg ctaccctctg gataacagac aactcctcag
    27901 cagattccac gttgatcatg aggatatcaa ccttcaccgc tgatatgatt tcctcttccg
    27961 tcttaccaac acctgaaaaa acgatcctct ggggttgtat acctgctctg agggcgcggt
    28021 agagttctcc tcctgatacg atgtctgccc cgccgccaag aaatcccatg acccttaata
    28081 tggcaatatt ggaatttgcc ttacaagagt aacacgtgag gtgtgggact cggttaaaag
    28141 cgctgtcaaa tacacggtaa tggcgctcca gtgttttata actgtaaatg tatacgggcg
    28201 ttcccacttc ctgtgcgatc tgcgccacag gaacgctctc tccgaacagt tcttcattct
    28261 tgtaatcaaa ataattcatg attcagctca ctctttcacg attactatta tttcccgaga
    28321 tggttcactt tcattacccg agatatctac tgatttgaca tagtaggata aaaaacgatg
    28381 ctccccaaga ttatggtcca tgaactgcgt ttcttttatg ggcgttttgg taagcttttg
    28441 ctctagcctg ccaacaaccc gataaacatt gtagccagcc atatcatctt ctttgttttc
    28501 aagccatgat atctggacaa cgcttcctct tttttcagcc tttattccgc gaggaaccgc
    28561 cgggggtgtt aaatctttag gggttgcctc aacgcgaatc ccttcccctt ccaagactct
    28621 tttttccact acttttacct tacgaatctc ataaacatat ttctgaccat tcttaagacc
    28681 tgcatcgaga aaaaatggac ttgacagggg gtccttattg atcggaaaaa gctggtagaa
    28741 cccattctca gaccggtaca cattgtagaa gaatcccttt tcctggtgcc atgataactc
    28801 gactctgcca tcgttttccc tgactgtcac attacctgca ggtttcgtgg gaggatcctc
    28861 ccaacggatg gtaaatattt ttgaagactc gcctaaaaca cctctggttg taaacggttt
    28921 gaccatgtac gagtacgtgt aaccatggta caggtcatca tcgtagatga aaatcttgtt
    28981 tcctgccatt gtataacctt tttcatcctc gaggcggatt tccctgaatg cttcaaatgc
    29041 tccctcgcag gttacgcagc gtttgagaat cttaaaacca gccaggtcca cagcgtccga
    29101 tccatccagg ttcttgatcg gcatggcaaa agagaggaac aagacaccat ccttaacctc
    29161 tcctgaaagt gtttcagccc ctgcaggcac cggctgctgt tttggtaaag ggtttccctt
    29221 cttgccacag gaaaatacga ggcacaggat tccgatgcta agaattaaga taatgtcttt
    29281 ccgcgacaat ctgtctctca acctcctcaa aggatgcggc ccccatagtt ttcctgtttc
    29341 tcaaaatggt aagtggatca atatgcgcta tgatgtcttt gtcaaaggct tctgagtagg
    29401 tcctgagatc ttcgagtttt agatttcgta gctgtttgcc cttatcctcg cagtcacgaa
    29461 caagctttcc tacaatgaca tgagcctgtc tgaagggtat acctttgagg gtaagatatt
    29521 cagccatctc aactgcaggc atgaaacttt catacacagc ctctttcatc ttttcgcgat
    29581 tgacagacat gcccttgata cagagttcca ctatggacaa tacatctttt acagtagata
    29641 tggcatggaa caatggttct ttatcctcct gaagatccct gttataggta aggggaagac
    29701 cctttaggag ggaaaatata ctgaagagat caccaatgac acgggaggtt ttacccctga
    29761 taagctcaag agaatcagga ttctttttgt ggggcatgag gctgctgccc gtgcaaagtt
    29821 cgtccggcaa tgagacaaag gcaaactcgt cagtagaaaa gatgatcaaa tcctctgcaa
    29881 gcctgctcaa gtgtatcatt atcatagcaa tcgagtaaat tgattcaaga acaaaatccc
    29941 tgtctgcgat tgtgtccata ctgttctctg agatagaaga gaaattgagc ctctccctca
    30001 caaactccct gtccagaggt atggttgatc cggctattgc accgcttccg agaggcagaa
    30061 catcaacggt tttccatatc tgtattaatc tttccaggtc acgctttagc atttgataat
    30121 atgcaagaag gtggtgagca aaggttgtta tctgggctct ctgcatgtgg gtataaccag
    30181 gcatgatggt ttccttttct ttttctgccc tggttataaa actgtgaagt acatttcgaa
    30241 ggagatttct tatttctccc aactctttct ttaggtacat gcgcatatcc agggctacct
    30301 gatcatttct actccgccct gtgtggagtt tttttccgat ttctccagct ttatctataa
    30361 gtctcgcctc gatatgcata tggatatcct caagagcatt agaaaaaaca aactctcctt
    30421 cttgaatctc tcttttcacc tcctcaagag cattgatgat gatctccctc tctttttcat
    30481 ccaaaatgcc gatcttgtga agcatttcgg catgggcttt gctaccctca atgtcatatt
    30541 catataacgc ccaatcaaaa ccaattgaag cactgaaccg ctccataaga ggctcggtct
    30601 ttcctttgaa tcttccaccc caggctttca ttgtctctct ccaaagatgg ctgttccaat
    30661 cctgaccatc gttgcacctt cttcaatagc tacctcgaaa tcagatgaca tccccattga
    30721 tagctcagtc atggaaagag aaaactcttt attcactctc tccaatattt gccgaaggcc
    30781 agaaaaaata cccctcacgt cctctgcatt ttctgtaaag ggaggcatgg tcataaggcc
    30841 caccggttca atgttttgca atgtgtgtat cctttcgagc atccttttca ggttatcctc
    30901 ctgggtccca tgcttcgact gctctcctga aaggttaagc tcgaacaata ccttcatggg
    30961 ctttccaaac ttttcaagcc tttctataat ctcccatcga tcaactgaat ggacatagct
    31021 gaaaagaccc ggaacatact tcactttgtt tgtctgtaaa tgaccgatca tgtgccagca
    31081 cggcccttca ccaaaatcgt ctatttttct ccgggcctcc tggatgtagt tttcaccaag
    31141 gtccctgatg ccacacgaga aagcaatacg tatcctctct acatccaccc tttttgtgac
    31201 agcaatcagc cttacatctt tttcagatct gcctgacctg atgcttgccc tggatattct
    31261 ctcccttatc ctctgtatgc cgtcttcaat ggtattcacg aaagaacgcc caaggacaga
    31321 agtgcttctg ctatttcaca gattggaaga cctaccacat tggtatagga acctttgatt
    31381 tccttaacca tatagccgcc cctcccctgc acagcatatg aaccagcctt tcccataggt
    31441 tcggatgtct taatgtaacg tataatctca tcatcggtca tatttttcat gaagactttc
    31501 gtttcaacga tattgcaata ggtaatgtcc tgcaagacat tgagaacaca aaaccctgtg
    31561 attaccttat gccatttacc acccaaggtt ttcagcgtgt taaaagcatc ccgctcattt
    31621 ttcggtttgc caagcacctt gcccttcagg acgacaatgg tgtccgcagc tataacccat
    31681 ttgtctggaa aatgctttcc aacctttctt gccttctcgt aagagatacg ctctacaaat
    31741 tctctatacg tttcatccct gtgcttcgtc tcatcaacat caggaggtat gatggaaaag
    31801 gatataccca gactcctcag aatatctacc cttcgagtgg attcacttgc aagtactatt
    31861 gagtctccat ccttaacttg aaacatcgct ttctcctatc gtaaaataga aaaatatatg
    31921 aaattctgtt gaatttcaag ttaaatatga gagcactacc tggctcatac ccggtagatc
    31981 tcagctcata gcaaaaaaaa tcaataaggg gcgacatgga taaaattatt caggtcataa
    32041 caacgtgtaa caataaagaa cttttagagg aaatcggcac gtatctcgtg gagaacagac
    32101 tggctgcatg cgctcaaatc ctgggcccaa taacgagtac ctactggtgg caaggggaga
    32161 tcgaacaggc aactgagtgg gtgtgcactc tgaaaactac tgaaggtctc tatgagaagg
    32221 ttgagactgc aatccttgaa ctccatcctt atgatcttcc tgagattata gccatagatg
    32281 tggaaaagtc tttaccggca tatgcagatt gggtaaggga cgaaactggt cagggaaata
    32341 aaggaaacac catatgagga accacgttat cattattggg gcaggactgg cagggatggc
    32401 agctgccatg agtgcccatg ataagggggc atctgtcacc ctcatagaca ggggatcgat
    32461 aggcttggga acaaactccg ccctcgccgg aggacaattc accggtccaa ctttcctgca
    32521 cagccctgaa gaattcattc aggataccct ggatacagga aggggcatta acaggaagcg
    32581 cctggtcagg cttgtggctt tcaaggcacc cgaggctttt gattttttgc gccaccttgg
    32641 actaaaactc attgaaacac ctacagcatt tagaatccaa tctccgaacc cagtggttat
    32701 ccccggtgtt acccttgtca ggacgctggc agaaaagata aaagagatga agcatattca
    32761 aatcttaact cgtttctatg taacgaaaat catcattgaa aacgggaagg ctacaggcgt
    32821 atgcggtttt gataaagagg gtagaaacag gactatacac ggcgatggca ttgtccttgc
    32881 cacaggaggg gcaggggcaa tttattcaaa aaatgataac cagaagacaa tcatggggca
    32941 aggctactac ctcgccagta gtgcaggact caacctttgg gacatggaat ttgtccagtg
    33001 ttacccgttt gtcatagcag aaccccacct accctctttg ctcgtctacc ctccgtaccc
    33061 tcacgaggcc aggcttgtga acggtaaggg tgaggacatt ctcgcacgat acggtattga
    33121 agatatcaat gatgccatta tgaaaaaacg ggatgagcta tcagtgattc tgaacgagga
    33181 atctacagta tctcgcgttc tcatggattt cagtgaagta cctgaccaca tgtgggatgc
    33241 gtaccccctt tctatccttg aaaaacttca ttttgatgtg agaaaaaagc cctttgctgt
    33301 ttcaccggct gttcacttct gcatgggtgg cattgaggta aacgagaagg ggcaaacaag
    33361 cttggaaggt ctgtatgcct gcggagaagt cacatggggg ctccatggag cgaaccgaag
    33421 gggaggaaac gctcttactg aatgtcttgt catgggaaga gttgccggca catttgcagc
    33481 aagatataga ggagaagaag aaagtgaaga ccatgacaac atgaatcatc aaaacgagca
    33541 tgctgattct gcacagggtg ggttgtttat ctcattcaga gaacttcgcg cgcatgtacg
    33601 taaccttgcc tgggaacatg caggtatcgt cagggatgaa gcgggcttaa agaaaggcct
    33661 gcttatgttg gacagcattg agggagagct aaaaaatgca atgtggacaa atgttcgtga
    33721 acgaatcata aaggaagacc ttttaagcgc tgtcttctct gttaaagcta tcctcagtgc
    33781 aagcatgggc agaaaagaaa gcagaggcag ttttattcgg aaagactatc caaaagaaga
    33841 caatgataat tggcgtaaga attcatgctt aacgtacaat ccacaaaaca actctttttc
    33901 ggtctcatac cacgatgcag aatggggcga ttgattgggt tgaacaggtt gatgtgcagg
    33961 aggaagtagt atgaaattga aaaacatggt agctttttgc accgcagcag ctggcgcagg
    34021 cataggacat tcaacagccc gcacgtttgc cagagaaggg gcaagcatcg ttatcacaga
    34081 tgtgcatctg gagcgctgcc gcgctgtggc ggatgaaatt caacatgatt ttggtgttga
    34141 tgccttagct atcaagtgcg atgtcaccaa ccgtgaagat gtagaagcgg ccatcaatag
    34201 gacactgaaa caatttgata gaattgacat actcttcaac aatgcgggaa ccaatcgtcc
    34261 atcgcagatc atcgacataa cagatgaggt ctgggatctg gtggtcaata caagcctcag
    34321 aggaaccttt tattgttgca gggcggttct tcctgccatg ataaaacaaa actttggccg
    34381 tatagtgagt actacatctg tggctgcctt caggggtctc aaagctggcc atgctcatta
    34441 cgcagctgca aaggcaggtg tcatggcttt cacccgctgc ctggccatgg aggccgcccc
    34501 gtaccatatt actgcaaaca cgattgcgcc gagcttcatc tataatgaat tcatccccca
    34561 tatttaccct caggatgaaa tcgatcggat gtatgatgag ataccgtacc cgcgcaaagg
    34621 cactccagaa gacattgcta atacagtgct ttttctggtc tctcaggaag gcgaatacat
    34681 aacaggccag accatctgtg taaccggcgg gagttggatg cgatgatacg agctaacgtc
    34741 aagtgttttg tgtcagcaag aagaccgggt atactccctg gcttat
//
LOCUS       DAOOMC010000008        34227 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdus sp. isolate AMR_MDS_2685
            NODE_1036_length_34227_cov_6.019841, whole genome shotgun sequence.
ACCESSION   DAOOMC010000008 DAOOMC010000000
VERSION     DAOOMC010000008.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864760
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdus sp. (wastewater metagenome)
  ORGANISM  Syntrophorhabdus sp.
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae; Syntrophorhabdus.
REFERENCE   1  (bases 1 to 34227)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 34227)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,729
            Genes (coding)                    :: 2,708
            CDSs (with protein)               :: 2,708
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 7 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34227
                     /organism="Syntrophorhabdus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1036_length_34227_cov_6.019841"
                     /isolate="AMR_MDS_2685"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2699756"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..661)
                     /locus_tag="PK125_01465"
     CDS             complement(<1..661)
                     /locus_tag="PK125_01465"
                     /inference="COORDINATES: protein motif:HMM:NF021127.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HPB36807.1"
                     /translation="MPRFFVHPLIILSIIFTLWGTQGCRNVCAGTSADNPLTIIAVGD
                     IMMGTIYPVDILPPDDGREMFKAIEGELKDGDIVFGNLEGSLCDRGKPVKCKVPLSRN
                     CFEFVMPARYGGYLKEAGFSVLNIANNHLLDAGFEGAECTIANLLDTGIQMTGGATVA
                     KFFKKGKKIAIPGFSYKASPYAHSILDIPGAQEIVAELKKDHDIVIVSFHGGAEGRTA
                     IH"
     gene            complement(709..1047)
                     /locus_tag="PK125_01470"
     CDS             complement(709..1047)
                     /locus_tag="PK125_01470"
                     /inference="COORDINATES: protein motif:HMM:NF021221.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4870 domain-containing protein"
                     /protein_id="HPB36808.1"
                     /translation="MEGNDLGKTSTGINPTVAGLLSYLLGFVTGIIFLVMEKENRFVR
                     FHAMQSILTFGFLFVINIVLHFIPFIGWALVPILGIVSLIVWILLMVKAYKGEYYKLP
                     VVGDMAEQKI"
     gene            complement(1115..2956)
                     /gene="glmS"
                     /locus_tag="PK125_01475"
     CDS             complement(1115..2956)
                     /gene="glmS"
                     /locus_tag="PK125_01475"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: protein motif:HMM:NF001484.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HPB36809.1"
                     /translation="MEEEEIVCGIVGYMGPGEASRILIEALKRLEYRGYDSAGIAIWN
                     EGKVEVARKKGKVEELRKEIADNEKFQGNLGLAHTRWATHGVPSERNAHPHRADDVIV
                     VHNGIIENYLELKDELRKAGQEFLSDTDTEVIPHLISSFMRKPMAFIDAIRQALTELQ
                     GSFAVGIINKKDRIMVAAKKESPLIIGVGDGEYFIASDAPAVLDRTNRFIFLEDGDMA
                     VFKDNQLSLMDFQRKEIVRPVQTVSWTGVMAEKGGYKHFMVKEIFEQPRAISDTLIGR
                     IREEQGEVGFEELELPDISKIRKIWMVACGTSYHACLIGKHIFEANLRIPVEIDIASE
                     FRYRDPIMGADSMLILVSQSGETADTIAAMREGKKKGAYTLSVCNVLGSTLAREADGV
                     VFTHAGPEIGVASTKAFTTQISVLYLLLLHMGQKLGIMDSDTVRDLISEIKKIPRKIQ
                     KILDDAHAVEELARKYMTCKDFLFIGRGVNFPTALEGALKLKEISYIHAEAYAAGEMK
                     HGPIALIDENLPVVVVSPVDQTYQKTCSNIEEVLTRRGRVILFTDDASHDMRQKVENA
                     ITVPETIYELEPILTVVPLQLFAYYIANFLGTDVDQPRNLAKSVTVE"
     gene            complement(2938..4005)
                     /locus_tag="PK125_01480"
     CDS             complement(2938..4005)
                     /locus_tag="PK125_01480"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HPB36810.1"
                     /translation="MRQLSVVILAAGKGERMLSSKPKVMHEIMGKPMVGYVVERAQEL
                     SPINTVVVLGYGKEKVREYIKGFDIVTAIQRGQKGTAHAVLSAEKPVKGHDVLVLYGD
                     VPLIESSTLKGFLNFFQKAGTVTFMTTDVMNPTGYGRVIMKKDCIEKIVEDVDARPSE
                     KKIKRINTGICAIPSHLFPLLKEIKADNRKGEYYLTDIYKILHKRGIDVKGYHHEKSS
                     DVLGINSKKELLDANIIMRDRILDDHMKNGVALLDRNVYIEEGVVIGRDTIIYPNTHI
                     SGKTWIGEGVSIGPNSFIRDSVIHNRVNVEGFVFMEGAEIEEGARVDPYTKILFKTLI
                     RKDSKVHECVKEMNTVWRKKK"
     gene            complement(3992..4729)
                     /gene="tatC"
                     /locus_tag="PK125_01485"
     CDS             complement(3992..4729)
                     /gene="tatC"
                     /locus_tag="PK125_01485"
                     /inference="COORDINATES: protein motif:HMM:TIGR00945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HPB36811.1"
                     /translation="MTDEKQPFFSHLKELRDRLVVCIIAVAIAFILTYYFKERLFYFL
                     MQPFVRVMPAQSSFIFTGITEAFITYFKISIVAALFVAAPVILYEFWMFVAPGLYENE
                     KKYVYPFIFSGSLCFILGALFCYFIVMPNIYHFFVSYAQDFVIPMPDLKSYMSLTLKL
                     LIIFGLIFELPLVAFYLSRAGIINARMLAKKRRYAILSVFIISAIITPPDVVSMVLVA
                     LPLWGLFELGILIAKVFGKKEAPHETA"
     gene            complement(4726..5016)
                     /locus_tag="PK125_01490"
     CDS             complement(4726..5016)
                     /locus_tag="PK125_01490"
                     /inference="COORDINATES: protein motif:HMM:NF011429.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatA/E family twin arginine-targeting protein
                     translocase"
                     /protein_id="HPB36812.1"
                     /translation="MFGIGLPEMIIILVVALLVVGPTKLPELARTLGKAFKEFRRMAD
                     DVKETFEQEVMKEEEVKTTDTEEGARVEEKILEEAKQPEALHSRSGNDNKPT"
     gene            5147..5572
                     /locus_tag="PK125_01495"
     CDS             5147..5572
                     /locus_tag="PK125_01495"
                     /inference="COORDINATES: protein motif:HMM:NF013629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HPB36813.1"
                     /translation="MKKYKNLCLKLTPQRLAILEYLEGNTDHPSASDIYNAVCKRFPT
                     MSFATVYNTLKALKGKASIQELTIDPDKKRFDPNTVHHHHLICTECRKIIDIETEFEL
                     DLPELQKHGFEITGNHIDFYGVCPECAGLALQEYGGQLH"
     gene            5632..6006
                     /locus_tag="PK125_01500"
     CDS             5632..6006
                     /locus_tag="PK125_01500"
                     /inference="COORDINATES: protein motif:HMM:TIGR00320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_process: GO:0019430 - removal of superoxide radicals
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="desulfoferrodoxin"
                     /protein_id="HPB36814.1"
                     /translation="MAERMEVYKCDVCGNIVEVLVGGKGQLVCCNQPMNKMTENTVDA
                     AKEKHVPVIEKVANGFKVKVGSVAHPMEEKHYIQFIELIVDGKIYRQFLSPGQAPEAV
                     FEITGDKAIAREYCNLHGLWKA"
     gene            6053..6628
                     /locus_tag="PK125_01505"
     CDS             6053..6628
                     /locus_tag="PK125_01505"
                     /inference="COORDINATES: protein motif:HMM:NF012436.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin family protein"
                     /protein_id="HPB36815.1"
                     /translation="MASLKGTKTEHNLLASFAGESQARNRYSYFSSQAKKEGFEQISW
                     IFADTADNEKEHAKRFFKFLEGGMVEITSAYPAGVIGTTAENLAEAADGENLEWSTLY
                     PEFAKVADEEGFPEIARVWREIAKAEVGHETRYRKLLANLKEGKVFKKDVPVKWRCRN
                     CGYISEGTAAPDRCPACDHELAYQELLAENY"
     gene            complement(6830..7177)
                     /locus_tag="PK125_01510"
     CDS             complement(6830..7177)
                     /locus_tag="PK125_01510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB36816.1"
                     /translation="MKKVVLFAFNGEPMCFVHVLLNALDMKEKGFEVRVVIEGAATKL
                     IPDLAAEGNPLSVPFKQARAENLIDGVCKACATKMDTVENARTMGLVLLDDMLGHPSM
                     AKYLKDGYEVITF"
     gene            complement(7242..8225)
                     /locus_tag="PK125_01515"
     CDS             complement(7242..8225)
                     /locus_tag="PK125_01515"
                     /inference="COORDINATES: protein motif:HMM:NF024981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter"
                     /protein_id="HPB36817.1"
                     /translation="MWKILASINKNLVVAIPSMMLLGFIAGFFFKVGFLQNLIIPFTF
                     LMIYPMMVVLRIRKVLEGGDMRAQVLTQIINFAVIPFVAFGIGLVFFPHRPFMALGLL
                     LAALVPTSGMTISWTGFAKGNLEAAVKMTVAGLILGSLATPFYVQALMAAHISVDIAN
                     VMKQIVFIVFLPMVAGYITQISLVRRFGQKAFQETWSPRFPPMSTLGVLGIVFVAIAL
                     KAEAIAQLPAVLLYILLPLLLLYGFNYFLSTVVGKMLLPRGDAIALLYGTVMRNLSIA
                     LAIAMNAFGPAASDAALVIAIAYIIQVQSAAWYVRLTNRFFGEPEVQKVHG"
     gene            complement(8239..9669)
                     /locus_tag="PK125_01520"
     CDS             complement(8239..9669)
                     /locus_tag="PK125_01520"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HPB36818.1"
                     /translation="MSKERLVVIGGVAAGMSAASSYRRLKPEAEAIVLEKDFFISYGS
                     CSLPYYVSDEVKDFNQLISLTPKVAIEERGIDVRTRHEVSDIDLEKKQVHVRNLEKNE
                     TFVLDYDKLVITTGGLPIRPPLPGIDLDHVFTIRTLLDGIAIKKFIDDWGAFEVCVGS
                     PECLYVNRFGNEKRPMKAVVVGGGYIGMEMCESLRKRGLEVSVVEKTDRVLGTMDGEI
                     TSIVEEKLLAEGVKLYKGTSVEGFKGLKGKVERVITDKGEFDADFVLLAIGARPNSDL
                     AKKAGIALGVAGAIQVDEYMQTNVPGVFAAGDCAEAMHLVTGKKAYIALGTTANKQGR
                     LAGENAAGLNKKFDGIVGTAVTKIFDLEVARTGLSSIDTKREGLKFFTSAIQGWSRSK
                     AYPAGKKMTVLYIVEEGTGRLLGTQMVGQEGVAHRIDTLAACLYGRMTIDDVARLDLG
                     YAPPFATVWDPILVGANVALKKMKQG"
     gene            complement(9803..10330)
                     /locus_tag="PK125_01525"
     CDS             complement(9803..10330)
                     /locus_tag="PK125_01525"
                     /inference="COORDINATES: protein motif:HMM:NF016064.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE thiosulfate transporter family
                     protein"
                     /protein_id="HPB36819.1"
                     /translation="MSELVYGLITGIIFGFLLQKGRVIRYDKQIGALRLIDLTIVKFM
                     LSSILIAMVGVYILKDLGIAKLSIKTTILGGNIVGGLLFGIGWGLFGYCPGTAVGALG
                     EGRWDVVWGLLGMLAGAAIYAEAFPFMKRTVLTWGNYGKITLPQIFGVNHWVVILILW
                     ILVIIMFWGIEKKKL"
     gene            complement(10327..10857)
                     /locus_tag="PK125_01530"
     CDS             complement(10327..10857)
                     /locus_tag="PK125_01530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012471001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(10453..10455),aa:Sec)
                     /transl_table=11
                     /product="YeeE/YedE thiosulfate transporter family
                     protein"
                     /protein_id="HPB36820.1"
                     /translation="MTEKNSEPKGWNPYLAGALTGLLLVFSVWFTGKYIGASTTFVRA
                     AGYVERTFNPEHVAALEYFKKELPKIDWQFLFVIGIFFGSLIASTTSKSFRVQAVPTM
                     WESRFGPGMAKRGVVAFIGGAIAMFGARLADGUPSGHGLSGSLQLAVSGYVALICFFI
                     GGLIVANIVYAGGDKK"
     gene            11229..11768
                     /locus_tag="PK125_01535"
     CDS             11229..11768
                     /locus_tag="PK125_01535"
                     /inference="COORDINATES: protein motif:HMM:NF014573.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HPB36821.1"
                     /translation="METVILFGSPHRDGNTIQIVNALSDGLKQKGYNVRMLYLNDLNI
                     RPCQGCYACIPKGTCKINDDMKDIRKYMIDSDLIVYATPVYWYGPSGQLKLAMDRGIS
                     FLDNEYSSRLKGKKVVTLMTCAEDNTDTFQPALDMFKKTFDLLGLEYAGSVEAPGCVV
                     GKKIKKEFIQKVKKLAKAI"
     gene            11768..12520
                     /locus_tag="PK125_01540"
     CDS             11768..12520
                     /locus_tag="PK125_01540"
                     /inference="COORDINATES: protein motif:HMM:NF013348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPB36822.1"
                     /translation="MSKNGHYYFTSKKVGKAIWDYRMLQEGDKVLVAVSGGKDSISLV
                     KIMKDRTKFVPIKYDVMACFVDMGFKWVNKDILIRYFEEESIPFVIAHPPESWKGDEE
                     SFGCFWCSWNKRKALFDTAHNLGCTKIAFAHHMDDIIETMLLNLFFQGEIGTMQPFQD
                     MFEGELAIIRPLAYVEEKELLKLSRQMNLPIIASACPHSKTSKRYLLKGMIAELKKHN
                     RNVKKNIFRSLQRVREEYLLGPQKNLQKFNEG"
     gene            12566..13360
                     /locus_tag="PK125_01545"
     CDS             12566..13360
                     /locus_tag="PK125_01545"
                     /inference="COORDINATES: protein motif:HMM:NF018047.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide kinase InaA family protein"
                     /protein_id="HPB36823.1"
                     /translation="MTKLQKIRIGSFVMYLLPDTVNPSQFLDKFLRTGNQANKGRGGL
                     KLISINNELFVCRKYLHGGVGRAVTKDLFLSEKRALNEIQIMLYLKENHFPVVEPVGI
                     LIGEDFPFRKPHIITRFEDNTGELLEFIKVSSRKKRLRAIRDLARYMWHLERLGIFHP
                     DLHLRNVLVKKNGRLIFLDFDKASKRTISKKNVETMIWRLNRFAEKWEIKGYFRISIL
                     ERLLFIRTYSKLSGYNLEEEMAKKLVTKKLLSRAGWFAESILYGKN"
     gene            complement(13441..14325)
                     /locus_tag="PK125_01550"
     CDS             complement(13441..14325)
                     /locus_tag="PK125_01550"
                     /inference="COORDINATES: protein motif:HMM:NF015251.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HPB36824.1"
                     /translation="MLIDLLLILTIKVFQLSLRILPENYQQGTGAFFGRVAFKILKKR
                     RNVAISNILRAFKHYTEREASEIALRSFEKLGINFVEALLLPYIPTKDYEKRFSIEHR
                     DYMDRALALNKGVIALVFHYANWEIMGIASSFFQNPVVVLARPLKRHRLIDRFMNHMR
                     ASTGLTVIPNVNTSRDVMRFLRENKIIAILGDQREKRSKGVYVDFFGESVPTNKGITM
                     IAMKTGTPVMPVYFIRKGFLRYVIRCNEPIEMERKGKIEELIRQNTRKINAFLESIII
                     ENPDEWFWVHRRWGRDKP"
     gene            complement(14333..14509)
                     /locus_tag="PK125_01555"
     CDS             complement(14333..14509)
                     /locus_tag="PK125_01555"
                     /inference="COORDINATES: protein motif:HMM:NF015899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trm112 family protein"
                     /protein_id="HPB36825.1"
                     /translation="MPISKELLDILCCPKCKGDIRLTNAQDGLICDACKLMYPIKDDI
                     PVMLIDEAISIDIE"
     gene            complement(14524..16239)
                     /locus_tag="PK125_01560"
     CDS             complement(14524..16239)
                     /locus_tag="PK125_01560"
                     /inference="COORDINATES: protein motif:HMM:TIGR00717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HPB36826.1"
                     /translation="MVDTGDNSVHKENTQEEMQELYETSMRSPQEGNIFTGKVINING
                     DTVIVDVGLKSEGKVSLSEFTNKAGESTVNEGDEVEVMVVGREREFGLLILSKQRVDT
                     IRAWQNIEKSLEDGTPVEGDIVSEVKGGFLVDIGVNAFLPISQVDVKPVKNPASFVGR
                     HLKFKVIKSNKRKGNVIISRRILMEEEKEKRKQEFWKNVKKDQVVYGFIRNITDYGAF
                     VDLGGVDGFLYLNEITWGRITHPKEYLRVGDEVKVKIIDIDLEKERISVSMKQLKSDP
                     WLKIEERYPLQSKVKGKVVGVVEYGVFVELEQGVEGLLHITEMSWDRRLKNPHKIVNK
                     GDWIELVVLNIDQERKRISLGMKQLLPDPWDEMEKLYPPGTILKGKVKNLTEFGMFVG
                     IGNGIDGLIHMSEVSYSRRKKAISDVYKKGTYVEALVLTVDKTQKKFSLSLKRLKDDP
                     WKGISTKYHIGDIIDGFITSITDFGVFVEIEEGIEGLIHLSEMDDLQGKNLSEMYKVD
                     DSVQVVILNIDEKDKRIGLSIKALRKSEEKTEEKVAHQEEGAFSTLGDILESAMNRDN
                     QDVTG"
     gene            complement(16249..17070)
                     /gene="ispH"
                     /locus_tag="PK125_01565"
     CDS             complement(16249..17070)
                     /gene="ispH"
                     /locus_tag="PK125_01565"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015968 - stringent response [Evidence
                     IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HPB36827.1"
                     /translation="METVKSEHSSFCFGVKRAIDRVMKEIERKSHAIYTLGPIIHNPQ
                     IVSMLKEKGVIPVDDVTDISDGTVVFRTHGIKKEEEEYIQKKGLESIDVTCPFVKRVR
                     KRAMYLKKHGYQVVIIGDKNHAEVKSVLSYLDNDGIVLQESCRIDARKIGAVSQTTLD
                     RETFTSVVTGLLDGAEEIRVYDTICRSTQVRQQEAVKLAGLVDVMLVIGGKNSSNTTK
                     LYKIAKKVQPKTYHIETDKDLKSDWFRGARKVGLIGGSSTPDFIIDLVDRRVKNL"
     gene            complement(17058..17780)
                     /gene="cmk"
                     /locus_tag="PK125_01570"
     CDS             complement(17058..17780)
                     /gene="cmk"
                     /locus_tag="PK125_01570"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: protein motif:HMM:NF014300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HPB36828.1"
                     /translation="MIPNNLIITIDGPSGAGKSTVAKMIATSLHYTYIDTGAMYRGVA
                     WAFEKRKSAASGQQQANSDDQMEKIDNLKEWLTNLDLKFEFENDARVYLDGEDISKKI
                     REPDISMLASKLSQNKTVREHLYIVQRELGKAGGIVVEGRDTGSVVFPDAHVKFYLDA
                     NPEERAKRRHAELFSKGTKVDIASVREDMERRDKNDTERNLAPLIIPEGAIHVDTSGL
                     DVKGVVEELYKHIYTIGEKWKQ"
     gene            complement(17902..18966)
                     /gene="hisC"
                     /locus_tag="PK125_01575"
     CDS             complement(17902..18966)
                     /gene="hisC"
                     /locus_tag="PK125_01575"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HPB36829.1"
                     /translation="MKLSVQEYIIKIPYYPKAAMYGSEDGWIRLSSNENPYPPSPEAI
                     SRMMDELFCVNRYPGNESELKDAISKHYGKEPENVLIGNGSNEIIELSLKAMKHGTKN
                     KVVISEPSFAFYEIASKIYGYETVKAPLVKMKVDLGVVRDCIDDHTRVVFLNNPNNPT
                     GTIFNGEKFTDFLKNLPTDVLVVVDEAYGEFVESRAFPDSFSYIDDYPVIILRTFSKA
                     YGLAGLRIGYGVGERSVISFLDRTKQPFSVNVMAISAAKAALMDQKYLKKVLNNNRKG
                     KKFLYKALKALSVEFIPTEANYILVKIGSTAESVTKRLYEEKILVRWMGSYNLDEYIR
                     VGIGTMDENIVFVEVLKRIL"
     gene            complement(18963..19622)
                     /locus_tag="PK125_01580"
     CDS             complement(18963..19622)
                     /locus_tag="PK125_01580"
                     /inference="COORDINATES: protein motif:HMM:NF004981.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol phosphate phosphatase
                     domain-containing protein"
                     /protein_id="HPB36830.1"
                     /translation="MIDLHTHSILSDGELIPSELVRRAKVKGYRIIGISDHVDITNME
                     HIVSAMVKLSTKMEFYEGITILPGVEITHVPKGMIKEMIAFARKLGIFYVVVHGETIV
                     EPVQEGTNREAIEGGADILAHPGIISEADVALAKEKGVLLEISGRRGHSLGNGRVGLL
                     AKQIGASLIFNTDSHSPSDLVTEEEARKVVVGAGLMAEDFYKMQQNALDFVKRVLRER
                     A"
     gene            complement(19625..20122)
                     /locus_tag="PK125_01585"
     CDS             complement(19625..20122)
                     /locus_tag="PK125_01585"
                     /inference="COORDINATES: protein motif:HMM:NF014618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nuclease family protein"
                     /protein_id="HPB36831.1"
                     /translation="MLKEMKVAGITVDPFTNTPIVILKDMEEKDVLPIWIGLLEASSI
                     ATAIENIQTPRPMTHDLLKNILDELGAKVIKIEVNDLKDNTYYALLHMEVNKKRIVID
                     SRPSDAIAIALRTGASIFVDEGVIKRAAKIDLSQKSGKVVTDTNEWEDILENLSTDDF
                     GKYKM"
     gene            complement(20116..21444)
                     /gene="miaB"
                     /locus_tag="PK125_01590"
     CDS             complement(20116..21444)
                     /gene="miaB"
                     /locus_tag="PK125_01590"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HPB36832.1"
                     /translation="MKFCIENFGCQMNDHDTEKMVHLLLSKGMERIQDVNRADIVIVN
                     TCCIREKAEQKFYSLMGRLRKIKRKKGTILGVTGCIAQQEKDGIGEKLPFIDFSLGPS
                     SIHRLGEAVDCAIAGERHLFDFSNNGCIDSLLIKPGTRRGKIKAYITIMKGCNNFCSY
                     CVVPYVRGREVSRNSSDILQEIENLALLGVKEVTLLGQNVNSYNKARDDLPFPDLLRC
                     INDIDSIERIRFITSHPKDMSLELIECFGSLSKLCESIHLPFQAGSDRILKLMNRGYT
                     IEEYRRKISLLRERCRDIAITADCIVGFPGESEEDFAGTLSLVEDIRFDGLFSFAYSP
                     RKNTVAATLPGSIPREIALERLAHLQKIQKAITLEHNRAMEGKITEVLVEDWSKNSKE
                     EVTGRTRTNKIVNFKGSSGMLGGFIDVEIVKGYANSLKGEVRKPIGGHTC"
     gene            complement(21549..22673)
                     /gene="fbp"
                     /locus_tag="PK125_01595"
     CDS             complement(21549..22673)
                     /gene="fbp"
                     /locus_tag="PK125_01595"
                     /EC_number="3.1.3.11"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: protein motif:HMM:NF014057.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-1,6-bisphosphate aldolase/phosphatase"
                     /protein_id="HPB36833.1"
                     /translation="MKTTLSIIKADIGSIGGHIKPSEQLMQRVKDFLKKGSKGLVNDF
                     FVCHTGDDVAILFAHSHGTGYEKLHKLAWDAFLAGTAVAKDQGLYGAGQDLLKDSFSG
                     NVKGMGPAVAEMEFEERPNEPFVFFAADKTDPGAYNLPLYLMFADPMYNSGLILSPNM
                     AKGFKFVIMDVNYTEGDRLIELNAPEELYDIAALLRDNERYVVESVWSRETGDIAAVV
                     STTRLHNIAGKYTGKDDPVMLVRVQGPFPATGEALAPFNIGHFVAGFMRGSHNGPLMP
                     VTMNSPVSFFDGPPVVCALGFCVHKGKLTEPADAFDHPYWDYIRTKVSRKATDIREQG
                     FAGPAMLPYSELEYGGIVKKMEKLEPKFKVKKETKAKKKK"
     gene            complement(22670..23419)
                     /gene="surE"
                     /locus_tag="PK125_01600"
     CDS             complement(22670..23419)
                     /gene="surE"
                     /locus_tag="PK125_01600"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: protein motif:HMM:NF001490.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HPB36834.1"
                     /translation="MLILLTNDDGIHSEGLTLLANNLSQKYDVFTVAPERERTCVGHA
                     ITLHKPLRIREVDRGMFATNGTPVDCVFLGVKAVLKRAPDLIISGINKGPNMGQDVNY
                     SGTVAAAQEGAFLGIPSLAVSLNARRNFYFADAIKIVQAIIEVVKENNFLESTFLNIN
                     VPNVPYSKMKGFMVTRLGKRIYNDKVIERTDPRGGKYYWIGGNGEQFELMEGSDFYAI
                     EHGFVSITPLGLDVTSIKSISRFQKYFRRGL"
     gene            complement(23607..23680)
                     /locus_tag="PK125_01605"
     tRNA            complement(23607..23680)
                     /locus_tag="PK125_01605"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(23644..23646),aa:Pro,seq:ggg)
     gene            23902..24789
                     /locus_tag="PK125_01610"
     CDS             23902..24789
                     /locus_tag="PK125_01610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB36835.1"
                     /translation="MDHEDILLIHMGGLGDMCLSESTFLSLSHHFQKNISALGYLRFF
                     NFFKEYFKTINNIESARWLYLFSDYTTEQTWKRIVFVGKDKNGALRKRWKAVSRDEMI
                     FVDMYPDNTYGPVDSEAGTLTSKIENYQLKNVMHIESYQLTQLEQCGIKPLKKNIKAL
                     PRNRVILYPEASITKSKWHYENFIELYYTLKGHGVETHIFESFGLNLPIAEKIVIEDL
                     ADVKTFFDSGGIFVSNDSGMAHFAGFCGLLTITIFSDFDSSIWHPRGENISLRLGYDN
                     VVATELVDTIYRCLAEETE"
     gene            24876..25460
                     /locus_tag="PK125_01615"
     CDS             24876..25460
                     /locus_tag="PK125_01615"
                     /inference="COORDINATES: protein motif:HMM:NF021252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-GCAxxG-C-C family protein"
                     /protein_id="HPB36836.1"
                     /translation="MKDNEQEIKEIIQRSFSLGVEYERTIRGCCQCTIAAIQDALGIR
                     NDAVFKAGSGLTAGGGVSCEGSCGGFTGGVMVMSSVFGRRRDKWENDQVDKDCAHNMA
                     RALLNKFNEKYGSHICRIIHHRLFDREFDLKDAREREMFERLGAHDDKCTSVVGTAAA
                     WATELILEELAIRKMSLNDIRKMQVSGNGVWGRS"
     gene            25740..26894
                     /locus_tag="PK125_01620"
     CDS             25740..26894
                     /locus_tag="PK125_01620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB36837.1"
                     /translation="MKKCVAWLSSVILCMASQIAFSATSNDYFPLKPGTVWEYTTDQG
                     GTAERIVLKKTAMVNGAETSVVKYVDEGLRTYFSSDLSGIYLHREYEPSVYISGAGRK
                     SIDVTFDPPLKFGNATFEIGDSFHSEGTAITRAAGRTFNFGYRADATVEGTETVSVPA
                     GTFDTIRVRMELTLMSGGYGDFTTTSVLNFAKDIGVVKDFSTDTDNVTTTEELIYTNA
                     NVYDLAIISITQPKRVTLSPASPTKTSFLKVKLQNKGPFIEIIEDVTILTNLITLTAE
                     SLGACPAPLPVLHAGKPQKPFPIAIKPGKTLTVYYNVTFDCANNATKNTPDFRFSAIV
                     NRTAIDDKDDTNPADNACLRPGNLPKDKGCGARKPDHTLGGEILTDVVIK"
     gene            27300..28559
                     /locus_tag="PK125_01625"
     CDS             27300..28559
                     /locus_tag="PK125_01625"
                     /inference="COORDINATES: protein motif:HMM:NF015413.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HPB36838.1"
                     /translation="MTYITDVYLSEILKKDVVDQFGRKVGALWDLTIVPGAELPSVTR
                     LILRKRRQLYAVPVQCLQHFNRFVITVDLSDTSPGSYSIKEGDILIRKHILDKQIVDV
                     NGAKVVRVNDLKLGDADGSVCVIGIDVGPNGILRRIDGGSLIQKALQTFNRPIKESVI
                     GWHYLQTMDPHLRHLTLNVAKKQLGELHPSDLAQILTEVAPDEGTEILASVDEELAGE
                     ALHEVPDEVREKILREMDKELISDILEEMPPDEAADILGGMPEEKSEELLSLMEKEDA
                     EEVKDLLSYEEDTAGGLMTTEFLDFTPDMTIEEAMTDIRLLIPDVEFIYYIYIVDNED
                     HLLGVVTLKKILTNPLYKTLQEVMTQNVKAVHFDTGRKEIAEMISKYDIIALPVVDDD
                     NRILGIITVDDIIDLLVPNPTRRRKKR"
     gene            complement(28573..29907)
                     /locus_tag="PK125_01630"
     CDS             complement(28573..29907)
                     /locus_tag="PK125_01630"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HPB36839.1"
                     /translation="MKLKLFFVVCLFLFSLCVSSLASGLTVEEERKYGKEIFYQIANS
                     VPINNDPFISLYLQDIKKRLEEVATLPFPITLTVIQSQTVNAFATVGGYIYMTTGLIS
                     FSETEEELAGVLAHELAHITRRHVARRVEKEKYINIGMLATMLAGILVGGDSQAKQAI
                     IAGSQTAAQALSLKYSRDDEEDADRTGAIFADKAGYNATGVADFLKRLRISSGEVTIP
                     QYLLTHPYHEERIIKIENTWADSKTKNNTTFYPYLVVRANVLYRPPGTGSKEIWLNKY
                     KKDKDSPLNAYGAILAYMSAGNISESVKIASNIKSPYRDLFLGEALVNGRKFADAVEI
                     LKYKHDPMSKMFLAKAYDGLGDVEMAAKTYRDLLPYGPVFPEIYYRIGMLTGRMGLEG
                     MGYEYLGKYYLEIGRYDLAKTNLEKAVNRYGINSRDAQDLLKILDQLKKTSY"
     gene            complement(29907..30977)
                     /locus_tag="PK125_01635"
     CDS             complement(29907..30977)
                     /locus_tag="PK125_01635"
                     /inference="COORDINATES: protein motif:HMM:NF012970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB36840.1"
                     /translation="MPPLFLPIVHNLSPIARFFQFYIAKPKRMVYKTFIVHFFIVIIC
                     LFLAIPCSLSAHEGITACSYLLVEKDTFQVISGRNYDHKHPPASTTKVLTTIVALENL
                     QGNETIVPNAQVVKIPASKLNLIPGRQYKAFDLIKGTMIESANDAAYTIGTHIGGSEE
                     SFAKIMNDKARSIGAFDSNFKNASGLFVEGQYTTCWDLAIMFRYALESDNFRDIIATR
                     YFLFNDSKKSIRYKNHNRLLFCFEPTIGGKTGFTRASKHCYVGAFEKEGRVYILSMLG
                     SRDLWGDAIKILQSLYSDLPSDREIRLAKSNSLTLTSYKQKTPRKEAVKKTTVKKTTV
                     KKKTAKYKTEKYKKRSKKSAAR"
     gene            complement(31054..33150)
                     /locus_tag="PK125_01640"
     CDS             complement(31054..33150)
                     /locus_tag="PK125_01640"
                     /inference="COORDINATES: protein motif:HMM:NF017857.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
                     /protein_id="HPB36841.1"
                     /translation="MARFLRFNILHALLACLALLVFTSFPALPPFPFFPPVAYAADEP
                     FLGSANWGGTGLMEIPTARVMKENTYRFGFAEVSPYRYYYGALGLFPGLEVDGRVTEV
                     VGVKAFETPSSGWYRDKAFDVKYRFLPESKYGPALALGIMDPQGTRLYPSQYLVASKQ
                     IYPFDFTIGFGNGRFGKKPLAPSNEDIKAEIFTDPKRWFKESQIFWGVQFSPSDRYSF
                     MMEYSPIKFHKQISDPARRKYFDKAVPSGFNFGFRWKPFKWAEVDVSYQRGEQFSLNL
                     STLFEIGKPLIPIYDESYKEKADDKGYSVETRLINALFASGFSNIQVKINVTDVFVEA
                     QNNRYFYVTKAIGIILKLVNDITPRHVENIHIIINDNGIPLFKFSAMRADIGDWQTEK
                     LSNDELIYLSSFDPSITGGSDTRVFHRSLIDYGAKMSIENYYDVADFFQYRFGLAAWG
                     SYRPWKGATFLAQPEWYPINNISTRTLPLVDAVRSDFALYKEKDVTLARLMFDQTYKA
                     TNQLYSRISAGYLELQYAGIYGEIATTIVDGRIMFGLGGSAVRKRDPDNVLKLSDHYT
                     QIYKTAFFNSRLNIPEYDVTLDMKAGRFLAGDIGAKFTISKFIKGVTISAWYSITDTS
                     NLKDPLNRGYHDKGISIAIPIRIFRGSDSKTSYTYVLSPWTRDVAQDVNQYESLFDFI
                     GRNIKIFFDKDSKWLN"
     gene            complement(33279..33665)
                     /locus_tag="PK125_01645"
     CDS             complement(33279..33665)
                     /locus_tag="PK125_01645"
                     /inference="COORDINATES: protein motif:HMM:NF008912.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HPB36842.1"
                     /translation="MKNYEELQIWQEGCRLAVDIYQLTTNEPFTKDFGLKDQIRRSSV
                     SIPSNIAEGKERETIKELVRYLCVAKGSAAELKTQLYIAQGVGYLNADIYLDLNERIA
                     LLSKQIGGFIRKLKSTHKEEIVDING"
     gene            complement(33761..>34227)
                     /locus_tag="PK125_01650"
     CDS             complement(33761..>34227)
                     /locus_tag="PK125_01650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB36843.1"
                     /translation="LAHLRLLKGSEYDIELEEGDTLYIPAENKVINVVGAVMSNGSFI
                     YNEKWGYEDYVQMAGGYSRHADEDNVFVMKVDGSARKLSRGFMNWSPLKKRWEIAGFN
                     ESRGVLEAGDSIVVPDRLERTAWLRNLKDITQILANAGVAAATIAVLYKTLD"
BASE COUNT         9455 a         8181 c         6921 g         9670 t
ORIGIN      
        1 tatgaatggc cgtcctgcct tctgcacccc cgtgaaacga aactatgaca atgtcatggt
       61 cttttttcag ctcggctaca atctcctgcg ctcctggaat atcgagaata gaatgtgcat
      121 agggagaagc cttgtaggaa aatccaggga tagctatctt tttccctttt ttgaaaaact
      181 tcgccactgt tgcaccgccg gtcatctgta taccagtgtc cagaagattg gctatggtgc
      241 attccgctcc ttcaaaacca gcatcgagga gatggttgtt tgctatgttt aagacagaaa
      301 aacctgcttc cttgaggtaa cccccatatc tggcaggcat aacgaactca aaacaattcc
      361 tactcagagg caccttgcac ttcacaggct ttcccctgtc gcaaagagaa ccttcaaggt
      421 ttccgaacac tatatctcca tcttttagtt ctccttcgat ggccttgaac atttctcttc
      481 cgtcgtcagg aggcaggata tcgactggat atatggtgcc catcataatg tcaccaacag
      541 caataatggt aaggggattg tctgcgctgg ttcctgcgca cacattcctg caaccctgtg
      601 tgccccagag cgtaaagata atactgagta tgatcaatgg atggacaaaa aacctgggca
      661 tcaggagtat gggaaaatcc tgtatctaca ataaattaca aaatattatt atattttctg
      721 ctctgccata tcgccaacaa cagggagctt gtaatattcg cctttgtagg ctttcaccat
      781 gagcagaatc cagacaatga gcgacactat acccagaatt ggcactagag cccagccgat
      841 aaagggtatg aaatgaagaa ctatgtttat aacaaacagg aatccaaagg ttaaaatgga
      901 ctgcattgcg tggaatcgga caaacctgtt ctccttctcc ataaccagaa agattatccc
      961 ggtcacaaat ccgagaaggt aactcagaag tccggcaact gttggattaa tacctgtcga
     1021 tgtttttccc aaatcattcc cttccatagg tacccccttt tttgaaatgt ttcgctatca
     1081 gcttacagct attagctact cgctacaaga aaaatcattc tactgtgacg ctcttggcaa
     1141 gatttctcgg ttggtcaaca tctgttccaa ggaagttcgc gatgtaatac gcgaataact
     1201 gcaagggcac cacagtgaga atcggctcaa gctcatagat cgtctccggt acagtaatag
     1261 cgttctctac cttctggcgc atgtcatgag aagcgtcatc agtgaagagg attaccctac
     1321 cccgccgagt cagaacctct tctatattgc tgcatgtttt ctggtatgtc tggtcaacgg
     1381 gtgatacaac aactacagga agattctcat cgataagggc aattggtcca tgtttcatct
     1441 ctcccgcagc ataggcctca gcatggatat aagatatctc tttcagtttc agcgcaccct
     1501 ccagtgcggt tggaaaatta acccctcgtc caatgaaaag aaagtctttg caggtcatat
     1561 attttcttgc aagctcttca acagcatggg catcatcgag aatcttctga atcttgcggg
     1621 gaatcttctt gatttcactg atgaggtctc ttactgtgtc gctatccata atgccaagtt
     1681 tttgtcccat gtgaagcaac agaagataga gaacggagat ctgtgttgta aatgcctttg
     1741 tagaggcaac acctatttcc ggccctgcgt gggtaaaaac cactccatcg gcttcccgcg
     1801 cgagggtact gccgaggacg ttgcatacag acaatgtata agcgcctttc ttttttcctt
     1861 cccgcatggc agcgatagta tcagccgttt caccggactg tgaaacaaga ataagcatgc
     1921 tgtcagcgcc catgataggg tcacggtaac ggaattccga ggcaatgtca atctcgacag
     1981 gaattctcag gttagcttca aagatgtgtt ttccaatcaa gcaggcatga taggatgtcc
     2041 cgcacgcaac catccaaatt ttgcgtattt tggaaatgtc aggcagctca agctcctcga
     2101 accccacctc cccttgctct tccctgattc ttccaataag agtatcagaa attgcacgag
     2161 gttgttcgaa tatctctttc accatgaaat gtttgtaacc gcctttttca gccatcaccc
     2221 ctgtccaact tacagtctgc acgggtctca caatctcttt cctttgaaag tccataagcg
     2281 agagctgatt atccttgaag acagccatat cgccatcttc cagaaaaatg aaacggtttg
     2341 tcctgtcgag gacagcagga gcatcacttg caataaagta ttctccatcc ccaacaccaa
     2401 tgatcaaagg gctttctttt ttcgcagcca ccatgatacg atccttttta ttgataatgc
     2461 caacggcaaa cgacccttga agctctgtaa gggcctgccg aatggcatca atgaaagcca
     2521 taggtttccg cataaaagag cttatcaaat gggggattac ctctgtatca gtatctgaaa
     2581 gaaactcctg ccctgctttc cgcagctcat ctttaagctc caaatagttt tcaatgattc
     2641 cgttgtgaac tacaatgaca tcgtcagccc tgtggggatg agcattccgc tctgacggaa
     2701 ccccatgggt tgcccaccgc gtgtgggcga ggcccaggtt accctgaaat ttttcattgt
     2761 ctgcaatctc ttttctgagc tcctccacct tacctttctt acgtgcaacc tcaactttcc
     2821 cttcgttcca gatggcaata cctgccgagt cgtagcccct gtattcaagc cttttcaagg
     2881 cttctatcaa aatgcgcgag gcttcaccgg gccccatata gcccactatg ccgcacacta
     2941 tttcttcttc ctccataccg tgttcatttc tttaacgcac tcatggacct tgctgtcttt
     3001 cctgataagt gttttgaata gtatctttgt atatgggtct accctggcgc cttcttcgat
     3061 ctctgcacct tccatgaaga caaaaccttc aacgttcact ctgttgtgga taactgagtc
     3121 ccgtatgaat gagttcggcc cgattgatac accctctcct atccatgtct tccctgagat
     3181 atgggtattt ggatagatga tcgtgtccct gccaatgaca accccttcct caatgtaaac
     3241 gttcctgtca aggagtgcta caccattttt catgtggtcg tcgagaatcc tgtctctcat
     3301 aataatgttc gcatcaagga gttccttttt tgaattgatg ccaagtacgt cagaagactt
     3361 ctcgtgatgg taccctttta cgtcaatccc tctcttatgc agtatcttat agatgtcggt
     3421 aaggtagtat tcgcccttcc tgttatctgc tttaatctct ttcaagagag gaaagagatg
     3481 gctgggaatt gcacatatac cggtgttgat ccgttttatc tttttctctg aggggcgtgc
     3541 atcgacatct tcaacaatct tttcaatgca gtcctttttc atgatgaccc gcccataacc
     3601 agtgggattc atcacatcag ttgtcatgaa tgtaacagtt cctgccttct gaaaaaagtt
     3661 gagaaagcct tttagggttg atgactcaat aagaggtaca tctccgtaaa gaacaaggac
     3721 atcatgcccc tttacaggct tttcagctga cagcacagca tgggcagtac ctttctgacc
     3781 cctttgaatg gcggtaacaa tgtcaaagcc ttttatatac tccctgacct tttcctttcc
     3841 atatccgagt acaactacgg tatttattgg tgaaagctct tgcgctcgtt caacaacata
     3901 tcccaccatg ggcttgccca taatctcgtg cataaccttt ggcttgctgg aaagcatacg
     3961 ctctcccttg ccagcagcaa ggataacaac gctaagctgt ctcatgagga gcctcctttt
     4021 ttccaaatac ttttgcaata aggatgccca gctcaaatag cccccagagg gggagggcca
     4081 caaggaccat acttaccaca tcaggggggg taatgatggc acttataatg aacacagaaa
     4141 ggattgcata cctcctcttt tttgccagca ttctcgcatt aatgatgcct gccctggaaa
     4201 gataaaacgc aacaagagga agctcaaaaa taagtccaaa aattatgagg agtttcagcg
     4261 tgagactcat ataacttttc aaatccggca taggaatgac aaagtcctga gcatagctga
     4321 caaaaaaatg atagatgttc ggcatgacaa taaagtagca gaacagggcg ccaaggataa
     4381 aacacaggct tccagaaaag atgaagggat atacgtattt cttttcgttt tcatagagtc
     4441 ccggtgcaac aaacatccaa aactcataca ggataacagg tgctgcaaca aaaagggccg
     4501 caacgattga tatcttaaag taggtgataa aggcttctgt tattccagta aagataaatg
     4561 aactctgggc aggcataacc ctgacaaaag gctgcatcaa aaaatagaaa agtcgttcct
     4621 tgaaataata agttagaatg aaggctattg ctacagctat aatacagaca actaacctat
     4681 cccgcagctc ctttaggtgg gaaaaaaatg gctgtttctc atctgtcatg tcggtttatt
     4741 gtcattgcct gatctggagt gtagtgcttc aggttgtttt gcctcttcca gaatcttttc
     4801 ttctaccctg gctccttcct ctgtatcggt tgtttttact tcctcttctt tcatgacctc
     4861 ctgctcgaag gtctctttca catcgtctgc catccggcga aattctttaa aagcctttcc
     4921 cagtgtcctt gcaagttctg gaagttttgt tgggcccaca acaagaagcg ccacaacaag
     4981 aatgattatc atttccggaa gtccgatacc aaacatacaa ggtaaaatta tcacaaaacg
     5041 ccatacagat aaagggaaat tgtgaatttg cacaataccg cttgacaata caggaatgtt
     5101 tatagtattt tttatcatgt aataattatt ctaaattgaa aggtcaatga aaaaatataa
     5161 aaacttgtgc ctgaagctta caccgcaaag gcttgctatt cttgagtacc ttgaaggtaa
     5221 tacagaccac ccttcggcct ccgacatcta taatgcagtg tgtaagaggt ttcccactat
     5281 gtccttcgcc acagtctaca atactctgaa ggcactgaaa ggaaaagcaa gcatacagga
     5341 attaaccata gatccggaca agaaacggtt cgatcctaat accgtgcatc atcatcacct
     5401 catctgcact gagtgcagaa agatcattga tatagaaacc gagtttgagc ttgaccttcc
     5461 tgaattacaa aaacatggat tcgaaataac aggaaatcat atcgattttt atggggtctg
     5521 ccctgaatgt gccggtctgg cattgcagga gtatggggga caactccatt gacaaccgga
     5581 aaggcgacaa accccggtgg aattgtaata aaaaaataaa ggaggtttac catggcagaa
     5641 agaatggagg tttacaaatg tgatgtatgc ggaaacatag tggaggttct tgtaggcggc
     5701 aaaggtcaac tcgtctgctg caatcagcct atgaacaaaa tgacggagaa tacagtagac
     5761 gcagccaagg aaaaacatgt cccggttatt gaaaaggtag caaacggctt caaggtgaag
     5821 gtcggcagcg tagcccatcc aatggaggaa aaacactaca ttcagttcat tgaactcatt
     5881 gttgatggga agatttaccg tcagttcctg tcaccaggac aggctccgga ggcagtattt
     5941 gagataacag gagacaaagc aatagcacgt gaatactgca acctccatgg actgtggaag
     6001 gcctaaaacc cgttacgcgt acgcgttacc agttaaaaag gaggataaca gaatggcatc
     6061 tttgaaaggt actaaaacag aacacaacct gctcgcttct tttgcaggcg agtcacaggc
     6121 acgaaacaga tacagctact tttcttccca ggcaaagaaa gaagggttcg agcaaatatc
     6181 ctggatcttt gccgacacag cagacaacga aaaggaacat gccaaaagat tctttaaatt
     6241 cctggaaggc ggcatggttg aaatcacttc tgcttaccct gctggggtta tcgggacaac
     6301 agcggaaaac cttgcagagg cagctgatgg ggaaaatctg gaatggtcaa ccctgtatcc
     6361 agaatttgca aaggtagcgg atgaggaagg ctttcctgaa atagcaaggg tctggcggga
     6421 gatagcaaaa gcagaggttg gtcatgaaac gcgttatcgg aaactccttg ccaacctgaa
     6481 agaggggaaa gtctttaaaa aggatgtacc agtaaaatgg agatgcagaa actgcggata
     6541 catcagcgaa ggaacggcag caccagacag atgccctgcg tgcgaccatg aactggcgta
     6601 tcaggagcta ctcgctgaaa actactagta tcccgtcttc attatgactg ttatcagcat
     6661 ttaaccgtca tgccgggttt tcctgagcag agcgtcgaga agcaatgaag ctgggctttt
     6721 ttcatattca aaaactccga agctgaactt gagcgagtgg agtcgctgga aatgaacgac
     6781 attgcttgca ggaatacccg gaatgatggt tatcctaaca aatttccaat caaaaagtaa
     6841 ttacttcgta accgtctttg agatattttg ccatggatgg gtgtcccaac atgtcatcca
     6901 gcaatacaag gcccatcgtt ctggcatttt ctacggtatc catcttggtt gcacatgcct
     6961 tgcacacacc gtcaatgaga ttttccgccc gtgcctgttt gaatggcacg ctgagagggt
     7021 tcccttccgc cgcaagatct gggatgagct tagtggcagc tccctctatg actaccctaa
     7081 cttcaaaacc cttttctttc atatccagcg cattgagaag tacatggaca aaacacatgg
     7141 gttcaccatt aaatgcgaaa agaacaactt ttttcataat atttcctccc tatcctccta
     7201 tctttttatt gaaaattttt acagtatcta cagcgctacc attatccatg taccttttgc
     7261 acctctggct caccgaagaa gcgatttgtg agacgaacat accatgctgc ggattgaacc
     7321 tgaataatgt atgcaatcgc tataactagt gctgcatctg atgcagcggg gccaaaggca
     7381 ttcattgcga tggcaagtgc aatagagaga tttcgcatca ccgttccata cagcagggct
     7441 attgcatcac cccggggaag caacatcttt cccaccactg tgctgaggaa ataattgaac
     7501 ccatagagaa gaaggagggg caaaaggata tacaagagaa cagcgggcaa ttgggctatt
     7561 gcctctgctt taagcgctat tgccacaaag actataccca gcactcccag cgtagacatg
     7621 ggtggaaaac gggggctcca cgtttcctga aatgccttct gaccgaatcg cctcactaaa
     7681 gaaatctgcg taatataacc tgccaccatg ggaaggaaga caatgaacac tatttgcttc
     7741 ataacatttg ctatatcaac actgatatgc gctgccatga gagcctgtac ataaaagggg
     7801 gtggccagcg atcccaggat tagccctgca accgtcattt tcaccgcagc ctccaggttg
     7861 ccttttgcaa aacctgtcca ggaaattgtc atcccgctcg ttggcacgag agcagccagc
     7921 agaagaccca aagccatgaa tggccggtga ggaaaaaata caagccctat tccgaaggca
     7981 acaaagggga ttaccgcaaa attaattatc tgggtcaata cctgtgccct catatctccc
     8041 ccctcaagga cctttctaat tctaaggaca accatcatag gataaatcat aagaaaggta
     8101 aagggaatga taagattctg cagaaacccc accttaaaga aaaatcctgc tataaaaccc
     8161 aacaacatca ttgaagggat tgcaacaacg aggttcttat ttattgatgc cagaattttc
     8221 cacataaagc aggaaaactc agccttgttt catctttttc agtgctacat tggcacccac
     8281 aaggatcggg tcccacactg tggcaaatgg tggagcataa ccaagatcga gacgggccac
     8341 atcatcaatg gtcatgcgac catagaggca tgctgcaagc gtatcaatcc ggtgtgccac
     8401 tccttcctgg ccaaccatct gcgttcccag aagccttccg gttccctcct ccacaatgta
     8461 aagcactgtc atcttcttcc ccgcgggata cgctttgctt cttgaccagc cctgaatagc
     8521 cgaagtaaag aatttaagcc cttcacgttt ggtgtcaatc gatgataatc cggtcctggc
     8581 aacttcaagg tcaaagatct tggtcacagc tgtacccaca ataccatcaa attttttgtt
     8641 aagcccggct gcattctctc ctgccagcct cccctgcttg tttgcagttg tccctagggc
     8701 aatgtatgcc ttcttgccgg tcacaagatg catagcctct gcacaatcac cggccgcaaa
     8761 aacaccgggt acgtttgttt gcatatattc atcaacctgg attgcgcccg caacgccaag
     8821 cgctattcct gctttttttg caaggtctga gttcggacgt gcaccgatag ccagaagaac
     8881 aaagtctgca tcgaactcgc ctttatcagt aataaccctt tccaccttgc ctttcaggcc
     8941 cttgaagcct tccacagatg tccctttgta gagcttcacg ccttcagcca gcaatttctc
     9001 ttctacaata gacgtaatct ccccatccat tgtgccgagc acacggtccg ttttctcaac
     9061 aaccgatacc tcgagaccac gctttcggag cgattcacac atctccattc caatataccc
     9121 gccccctact acaactgcct tcataggtcg cttctcatta ccgaaacgat ttacatatag
     9181 acattccggg gaaccaacac acacttcaaa agcaccccaa tcgtcgatga atttctttat
     9241 agcaatgccg tctaagagag tacggatggt aaacacatga tcaagatcaa tgccgggaag
     9301 gggaggcctg atgggaagtc cgccagttgt aattacaagt ttatcataat caaggacaaa
     9361 ggtttcgttt ttttccagat tccttacatg aacctgcttc ttttcgagat ctatgtctga
     9421 gacttcgtga cgcgttctca catcaatccc tcgctcttct attgctactt tgggggtaag
     9481 ggaaataagc tgattgaaat ctttcacctc atcagatacg taatacggga ggctacatga
     9541 tccgtatgaa ataaaaaagt ccttttcaag gacgatagcc tctgcttccg gttttagtcg
     9601 cctgtagctg cttgctgcgc tcataccggc agcaacaccg ccgattacta ctaatctctc
     9661 tttgctcatc tatctaccct ccaatgtgat atgttgtaca aagattcctt tccactatta
     9721 ataaaatttt catataacct atgtcgaagc actattggca gattccagga aacgatatgc
     9781 cacatacaat ttgaactata cactacagct tcttcttctc aattccccag aacattatga
     9841 ttacaagaat ccacaatatc agaatgacta cccagtgatt aacgccgaaa atctgtggca
     9901 gggtaatctt tccatagttc ccccaggtga gaactgttcg tttcataaag ggaaaagcct
     9961 cggcgtagat ggcagcgcct gcaagcatcc cgagaagtcc ccatactacg tcccatctcc
    10021 cctctccgag tgcacctact gctgtgccag gacaatatcc aaaaagcccc cagccaatac
    10081 caaacaggag gccccctaca atatttccac ccagaatggt tgtttttatt gagagctttg
    10141 caatgcccag gtctttcagg atgtatacac ccaccattgc gatgagaatg gaggagagca
    10201 taaacttgac tatagtcaga tcgatgagcc gcagggcacc tatctgtttg tcatagcgga
    10261 tgaccctgcc tttctgcagg aggaaaccaa agataatgcc tgttattaat ccatagacaa
    10321 gctcactcat tttttatcac ctcctgcata gacaatatta gcgacaataa gaccaccgat
    10381 aaaaaaacag atcaatgcaa cataaccgct gactgccagc tggagcgaac cgctcagccc
    10441 atgaccgctc ggtcacccgt cagccaggcg ggctccgaac atagctatgg caccaccaat
    10501 aaaggcaaca acaccccttt ttgccatacc cggaccaaag cgggactccc acatagtagg
    10561 cactgcctgt actcgaaaac tttttgacgt agtagaggca ataagggatc caaaaaatat
    10621 ccctatgaca aagagaaact gccagtcaat tttgggcaac tctttcttga agtattcaag
    10681 agctgctacg tgttcaggat taaatgtcct ttcaacatag cctgctgccc ggacaaatgt
    10741 tgttgaggcg ccaatgtatt taccggtgaa ccatacggaa aatacaagga gcaatcctgt
    10801 aagggcacca gccaggtaag gattccaccc ttttggttca gaattctttt cagtcataac
    10861 cttgcctcct ttcttgtgtt atgagccttt aaatttcaga tgtgcataca atatagggtt
    10921 attaccagca tttatcaaga caccacggct attttcctct acagacttca ttcaatttcc
    10981 attgcccagc agtgaactgg caacggtcaa cagtatatga agcaggctat agactatggc
    11041 aaagacgtaa aaaaagaagt aataaacaaa tcaattggct cttttaaatt aagtaaaatg
    11101 atgatctttc tttcatcttt tcactttgaa cttttaactt ttcactgtta tcagctcatt
    11161 ctcatcactt gaccctgtct atttattgcc attataatag gggatactaa acaggaggag
    11221 gtagccatat ggagacagtt attctgttcg ggagccctca tagggatggc aacaccatcc
    11281 agattgtgaa cgccctgtcc gatggcctga agcaaaaagg ctacaatgtg cggatgctct
    11341 atctcaatga cctgaacata agaccctgtc agggttgtta cgcatgcatt ccaaagggaa
    11401 cctgcaaaat caacgacgat atgaaagaca tcaggaaata catgatagac tcggacctca
    11461 tagtctatgc cacgccagtc tattggtatg gcccatccgg ccagctaaag ctcgccatgg
    11521 atagaggtat ctcatttctg gacaatgaat acagttctcg actgaagggc aaaaaggttg
    11581 tcaccttgat gacatgtgct gaagataata ctgatacttt ccagcctgcc ctcgacatgt
    11641 tcaagaagac tttcgacctt ctgggactgg agtatgcagg aagcgtagaa gcgcccggtt
    11701 gcgttgtagg aaaaaaaatt aagaaggaat tcatccagaa agtgaagaaa cttgcaaagg
    11761 caatctaatg agcaagaacg gccattatta ttttacatcg aaaaaagttg ggaaggctat
    11821 ctgggactac aggatgctgc aagaagggga taaagtcctc gtagctgtgt ccggtggtaa
    11881 ggatagcatc tctcttgtga aaatcatgaa ggatcgaaca aagttcgtac ccatcaagta
    11941 tgatgttatg gcatgttttg ttgacatggg tttcaaatgg gtgaacaagg atatccttat
    12001 cagatatttc gaagaagaat cgattccctt tgtgattgca catccacctg aatcatggaa
    12061 gggagatgaa gaatcatttg gctgtttttg gtgcagttgg aacaaaagga aggccctctt
    12121 tgatactgct cataatctgg gatgcacaaa gattgccttt gcccatcaca tggatgatat
    12181 tatcgagacc atgctcctca atctcttctt ccagggtgag ataggcacca tgcagccttt
    12241 tcaggatatg tttgaagggg agcttgcgat tattcgtccc ctggcctacg ttgaagaaaa
    12301 agaacttctt aagctctctc ggcaaatgaa cctgccaatc atagcctctg catgtcctca
    12361 cagcaaaacc tctaaacgat accttctcaa gggcatgatt gccgaattaa agaagcacaa
    12421 cagaaatgtg aaaaaaaaca tatttcgcag ccttcagcgt gttagagaag aatacctgct
    12481 tggcccacaa aaaaaccttc aaaagtttaa tgagggataa ggcaggatta aaggattgga
    12541 gagataagga ttaataaacc tgtaaatgac taaactgcag aaaataagga tcggttcttt
    12601 tgtcatgtac ctgcttcccg atacagtaaa cccctctcag tttcttgaca aattcctgcg
    12661 caccggaaat caggcgaata aggggagagg tggcctgaag ctcatctcca taaacaacga
    12721 gctctttgta tgcaggaagt atttgcatgg aggcgttggg agagcggtga caaaagatct
    12781 tttcctatcg gagaaaaggg ccctcaatga aatacaaatt atgctctacc tcaaagaaaa
    12841 tcattttccc gtagtggagc ccgtaggtat tctcattgga gaagattttc cctttagaaa
    12901 accccacatc ataaccagat ttgaggacaa cacaggcgag ttgctggaat ttattaaggt
    12961 gtcgagccgc aaaaaaaggc tgagggctat acgagatcta gccagatata tgtggcacct
    13021 tgaacggctt ggcatctttc atcccgatct ccatctgcgc aatgtgctgg taaaaaaaaa
    13081 tgggaggctc atcttcctcg acttcgataa agcttccaaa agaaccattt caaaaaagaa
    13141 tgttgagaca atgatatgga ggctcaacag gtttgctgaa aaatgggaga tcaaaggtta
    13201 tttccgcatc agcattttgg agagattgct ttttatccgg acatattcaa aattgtctgg
    13261 ttataacctt gaggaggaaa tggcaaaaaa actcgttacc aaaaaactgc tgtcccgggc
    13321 aggttggttt gcagaatcca ttctctatgg gaagaactga tgatggggaa agctccaaac
    13381 gtcaggctaa gcactgagaa ccaggaactc ataactaata accaacaaat gttaacatac
    13441 ctatggtttg tcgcgccccc accttcgatg aacccagaac cattcatcag gattttcaat
    13501 tattattgac tccaggaatg cattgatctt cctggtattc tgccggatca attcctcaat
    13561 tttacccttt ctttccatct caatgggctc gttacacctg atgacatacc tgagaaaacc
    13621 cttgcggatg aagtatacag gcatgaccgg cgtgccggtc ttcatggcaa tcatagtaat
    13681 ccctttattt gtaggcactg attcaccgaa gaagtctaca tatacaccct ttgaccgctt
    13741 ctctctctga tcaccgagga tagcaatgat cttgttttcc ctgagaaacc tcatgacatc
    13801 cctactcgta ttcacatttg ggattacagt aagtccggta gatgcccgca tatgattcat
    13861 aaatctatcg attagcctgt ggcgttttaa gggacgggca aggacaacga ccggattttg
    13921 gaagaacgac gacgcgattc ccataatctc ccagtttgca taatgaaaaa ccagtgcaat
    13981 cacacctttg tttaatgcaa gagccctgtc catataatcg cggtgctcaa ttgaaaaccg
    14041 cttttcatag tcctttgttg ggatataagg caggaggagg gcttctacaa aattgattcc
    14101 cagtttctcg aaagatctta gcgctatttc gctggcttcc cgctctgtat aatgcttgaa
    14161 agcgcggaga atatttgata tggctacatt tcttcttttt ttaagaattt taaaagcaac
    14221 ccttccaaaa aaagcgcctg taccttgctg gtaattctct ggaagaatac ggagagatag
    14281 ctgaaagact ttgatcgtta gaataaggag taaatctata agcacacgga aactattcta
    14341 tatctataga aattgcttca tctatgagca taaccgggat gtcgtctttg atagggtaca
    14401 taagcttaca ggcatcacag ataaggccat cctgggcgtt ggttagcctt atatcaccct
    14461 tacattttgg acaacaaagt atatcaagca attccttact gattggcata aataagtccc
    14521 cccttatcca gtaacgtcct gattgtccct gttcatggct gactcaagta tgtcgccgag
    14581 agttgaaaag gctccttctt cctggtgagc tactttttcc tctgtctttt cctctgattt
    14641 cctcaaggct ttgatactga gaccaatcct cttatctttc tcgtcaatat tgaggattac
    14701 tacctgaaca gaatcatcga ctttgtacat ttcggataga tttttcccct ggagatcatc
    14761 catttcagac aggtggatta agccctcaat accttcttca atttctacaa acacaccgaa
    14821 gtcagtgata ctcgttataa acccgtcaat aatatcccca atgtgatact ttgtagaaat
    14881 acccttccat gggtcatctt tcaatcgttt cagactcagg gaaaacttct tttgtgtttt
    14941 gtcaacagtt aacacaagcg cctcaacata agttcccttc ttgtaaacat cggaaatggc
    15001 ttttttacgt ctcgaatagg aaacctctga catgtgtatc aatccgtcaa tgccgttccc
    15061 gatacccaca aacataccaa actcagtgag attttttact tttcctttca gtatggtgcc
    15121 gggaggatat agtttttcca tctcgtccca cgggtcaggg aggagctgtt tcattccgag
    15181 ggatattctt tttctttcct ggtctatatt caagacaaca agttcgatcc agtccccttt
    15241 atttacgatc ttgtgggggt ttttcaacct tcggtcccaa ctcatctccg taatatggag
    15301 caggccctcc acaccctgtt cgagctccac aaagacaccg tattcaacaa caccaactac
    15361 ctttcccttc actttagatt ggaggggata tctttcctct atcttcaacc acggatctga
    15421 cttcaactgt ttcatgctaa cagagattct ttctttctca agatcaatat ctataatttt
    15481 taccttgact tcgtctccaa ctctcagata ttcctttggg tgggttatcc ttccccatgt
    15541 tatctcgttc aggtacagga atccgtcaac gccgccgaga tcaacaaagg cgccataatc
    15601 ggtgatgttc ctgataaatc cataaacaac ctggtccttc ttgacgtttt tccagaactc
    15661 ctgctttctc ttctcttttt cctcttccat gaggattctt ctggagataa tgacgttgcc
    15721 tttgcgtttg tttgatttga taactttaaa cttgagatgc ctacccacaa aagatgccgg
    15781 attcttaacc ggtttcacat cgacctgtga aatggggaga aaagcattca caccgatatc
    15841 gacaagaaaa ccgcctttaa cttcagagac aatatcacct tccaccggag taccgtcttc
    15901 cagcgatttt tctatatttt gccatgccct gatcgtatct accctttgct tggaaagaat
    15961 gagaagaccg aattcccttt ccctccccac aaccattact tctacttcat ccccctcatt
    16021 tacagttgat tcaccagctt tattggtaaa ctcactcaaa gagaccttcc cttcagattt
    16081 caggcctaca tcaacaatta cagtatctcc atttatgttg atgacctttc ctgtgaagat
    16141 gttcccttcc tggggactcc tcatggaggt ttcgtaaagc tcctgcattt cctcctgtgt
    16201 attctctttg tgcactgagt tgtcaccggt atcgaccatg ccgtccccct aaagattttt
    16261 tacacgccta tccacaagat ctataataaa atcaggggtt gaggaacccc ctatgaggcc
    16321 aacttttcga gcacctctaa accaatcaga tttaagatct ttgtccgttt ctatgtgata
    16381 cgtttttggc tgaacttttt ttgctatctt gtaaagcttt gtggtgtttg agctattctt
    16441 cccacctata acaagcatca catcaaccaa cccagcaagc ttgacagcct cctgctgcct
    16501 gacctgggta ctcctgcata ttgtgtcata caccctaatc tcttctgcac cgtctaaaag
    16561 cccggtgaca acgcttgtaa aagtctcccg atcaagcgtt gtctgactaa cagccccaat
    16621 cttccgggca tctatccggc aggattcttg caatacaata ccatcattat ccaaataact
    16681 caacacactt ttcacttcag cgtggttctt gtctccaatg attacaacct gatagccatg
    16741 ttttttcaaa tacatagccc gttttctcac ccttttcaca aaagggcatg ttacatcaat
    16801 gctttccagg cccttttttt gtatatactc ttcttcttct ttctttatgc catgggttct
    16861 aaagacaacc gtcccatcgc taatatccgt gacatcatcc acagggatta cgcctttttc
    16921 cttcaacatt gatactatct gggggttgtg aataataggg ccaagagtat atatggcatg
    16981 gctctttctt tcaatctctt tcattaccct gtcgatagcc cttttcaccc caaaacaaaa
    17041 actcgagtgt tcgcttttca ctgtttccat ttttcaccga tagtatagat gtgtttataa
    17101 agttcctcca cgactccctt tacatcaagg cctgatgtgt ccacatgaat agctccttca
    17161 gggatgatga gaggcgccag gttccgctca gtatcatttt tatcccgcct ttccatatct
    17221 tccctcacag atgcaatatc aaccttggta ccttttgaaa aaagttctgc atggcgcctt
    17281 ttcgcccgtt cttctggatt ggcatcaagg tagaatttca catgggcatc tgggaacaca
    17341 acgcttcctg tatcacgtcc ttcaacaaca atgcctccag cttttccaag ctcacgctgc
    17401 actatgtaaa gatgttccct aaccgtcttg ttctgtgaga gttttgacgc gagcatagat
    17461 atatcaggtt ctcgaatctt ttttgatatg tcttcaccat ccaggtagac cctggcatca
    17521 ttttcaaatt caaatttaag atccaggttg gtgagccatt ctttcagatt atcgattttc
    17581 tccatttggt catcgctgtt tgcttgctgc tggccgctgg ccgctgactt ccgtttctcg
    17641 aacgcccacg ctaccccacg gtacattgca cctgtatcta tgtaagtata gtgcagactg
    17701 gtagcaatca tctttgccac agtgctcttc ccggcaccac tgggtccatc tatggttata
    17761 atcaaattat tcggtatcat gcttggcaca cattcgatag agaatattac ccttatacaa
    17821 gcagaagttg tgaggccaag aaggtgaaaa gatgaatttt gaactttagc ttcttaactt
    17881 ctcaacctct gcccttttct attaaagtat cctcttcaac acctcaacaa atacaatgtt
    17941 ctcgtccata gtacctatac cgaccctgat gtattcatca agattatatg accccatcca
    18001 tcgcacgaga atcttttctt cataaagccg ttttgtgaca gactcagcag ttgatccaat
    18061 ctttacgaga atataatttg cctcagtagg gatgaattca acggacaggg ctttcagggc
    18121 tttatagaga aatttcttgc ctttcctgtt attattgagg acttttttga ggtacttctg
    18181 gtccatcaaa gctgcctttg cagcactaat agccatcaca ttgacactga aaggctgttt
    18241 cgtacgatcc aggaaggaga ttaccgatct ctccccaacc ccatacccga ttctgagacc
    18301 agccaaacca taagccttgg agaaagtcct gaggatgatg acgggataat cgtcgatata
    18361 agaaaaactg tcaggaaatg ctctgctctc aacaaattca ccgtatgctt catcgacaac
    18421 tacaagaaca tcagtgggca ggtttttcaa aaagtctgta aacttttcac cgttaaagat
    18481 tgtacccgtt gggttgttgg gattgttgag aaaaacaacc cttgtatgat catcaataca
    18541 atccctcaca actccgaggt ctactttcat cttcactaaa ggagccttta cagtctcgta
    18601 tccgtatatc ttggacgcaa tttcataaaa ggcaaaggag ggctctgaaa taacaacctt
    18661 gttttttgtg ccatgtttca tggcctttaa agacagttct atgatctcgt tagagccgtt
    18721 tccgattaag acattctcag gttccttgcc ataatgcttg cttatagcat ctttcaactc
    18781 agactcattg ccagggtacc tgtttacgca aaaaagttca tccatcatgc gtgaaatggc
    18841 ttcaggggac ggaggataag gattttcatt ggatgacaga cggatccatc catcttcaga
    18901 gccatacatg gcagccttcg gatagtacgg tatctttatt atatattcct gaacagagag
    18961 cttcatgccc tctctcttaa gactctttta acaaaatcaa gggcattctg ctgcatctta
    19021 taaaaatcct cagccataag acctgcgcca acaacaacct tgcgagcttc ttcttctgtg
    19081 acaagatcag atggactgtg ggaatcagta ttaaaaataa gggaggcacc aatctgtttg
    19141 gccaacaaac caaccctgcc attacccaaa gaatgccccc ttcttccgct tatttcaaga
    19201 agaacgcctt tctcctttgc cagggcaacg tctgcttcag agatgattcc cgggtgggca
    19261 aggatatcag ctcctccttc aatggcctcc cggttcgtac cttcctgaac aggctctaca
    19321 attgtctctc catggaccac aacatagaat atacccagtt tccttgcaaa ggcgatcatc
    19381 tctttgatca taccttttgg aacatgggta atctcaacgc ccggcagaat ggttatccct
    19441 tcatagaatt ccatcttcgt gctgagcttc accatggcag aaacaatgtg ttccatgttc
    19501 gttatatcca catggtctga tatgcctata attctgtatc ccttaacttt tgccctcctc
    19561 accagttcag agggaataag ttctccgtca cttaagattg agtgcgtgtg aagatctatc
    19621 atggctacat tttatacttc ccaaagtcgt ctgttgaaag attttcaaga atatcttccc
    19681 actcgtttgt atccgttacc accttgccac ttttctgaga aagatctatt tttgctgctc
    19741 tttttatgac accctcatca acaaagattg atgcccctgt tcggagagct atagcaatag
    19801 cgtcgctcgg tcgggagtca attacgattc tctttttgtt gacttccatg tgaagcaatg
    19861 cataataggt attatccttc aggtcattga cctcgatctt gattactttt gcccccagtt
    19921 catcgaggat gtttttcaaa agatcatggg tcatgggcct tggagtctgt atgttctcaa
    19981 ttgccgttgc tatgctcgag gcctccagaa gacctatcca gataggaagg acatccttct
    20041 cttccatgtc cttgaggata actatggggg tgttggtaaa agggtcaacg gttattccgg
    20101 caaccttcat ctcttttaac atgtatgacc tcctattggt ttacgaacct ctcccttcag
    20161 agaatttgca taacctttta ctatttcaac atcaatgaag cccccaagca tgcctgaaga
    20221 ccctttgaaa ttaacgattt tgtttgtcct tgtccttcct gtgacttcct ctttagaatt
    20281 tttgctccag tcctccacca gtacctctgt tattttccct tccatggctc tgttatgttc
    20341 gagggtaata gctttttgaa ttttctggag atgggcaagt ctttcaaggg ctatctccct
    20401 tggtattgat cccggcaggg ttgctgccac agtattctta cgtggtgaat aggcaaatga
    20461 aaaaaggcca tcgaacctga tgtcttcaac cagactcagg gttccggcaa agtcttcctc
    20521 actttccccc gggaaaccaa caatgcagtc agcagtgatt gcaatgtccc ggcatctttc
    20581 tctgagaagc gagatttttc tccgatattc ttctattgtg tatcctctgt tcattagctt
    20641 taaaatcctg tcagaacccg cttgaaacgg caggtggatc gactcgcaga gtttgctcag
    20701 actaccaaaa cattcaatca gttccaatga catgtctttt ggatgggatg ttataaatcg
    20761 aatcctttcg atgctgtcta tatcattaat acatctcaaa agatctggaa acgggaggtc
    20821 atcccttgcc ttattgtacg agttaacatt ttgccccaga agagtcactt ctttcacgcc
    20881 aagcagcgcg aggttttcaa tctcctgtaa aatgtcagaa ctgtttctgc ttacttcgcg
    20941 ccctcttaca taaggtacaa cacagtaact gcagaagttg ttacacccct tcattatcgt
    21001 tatatatgcc ttgatcttac ctctcctggt gcctggttta atgaggagtg aatctatgca
    21061 tccattgtta gaaaaatcga atagatgtct ttccccggcg attgcacagt ccaccgcctc
    21121 tcctaacctg tgaatgcttg aaggacctaa actaaaatcg ataaagggca atttctcccc
    21181 tatgccatcc ttctcctgtt gggcaatgca accggtaacg cccagtatag tccctttttt
    21241 tcgctttatt tttcttaaac ggcccatgag gctgtaaaac ttttgctcag ccttttccct
    21301 aatgcagcac gtgttgacaa ttacgatatc tgctctgttc acatcctgga tgcgttccat
    21361 gcctttcgat aaaagaagat ggaccatctt ctctgtatcg tgatcattca tctggcatcc
    21421 aaaattttca atacagaact tcatgtcagt ttcaatccat ataaaaatcg ttgcaggtta
    21481 catgtttcaa gtgaaaccgg taacctgcaa cgtgcaacgt agttaaagac gatattctgc
    21541 aataaagttt atttcttctt ttttgctttt gtctcttttt tcactttaaa ctttggctca
    21601 agcttctcca tttttttaac aattccacca tattcaagct cggagtatgg aagcattgca
    21661 ggacctgcaa atccctgttc cctgatgtct gttgccttcc ttgaaacctt agtccttatg
    21721 tagtcccaat atggatggtc aaaggcgtct gcaggttcgg taagcttacc tttatgtaca
    21781 cagaaaccaa gggcacaaac aaccggtggt ccatcaaaga atgagacagg cgagttcatg
    21841 gtgaccggca ttaaggggcc gttgtggctt cctctcataa aacccgcaac gaaatgaccg
    21901 atgttaaagg gtgcaagagc ctctcctgtt gcagggaacg ggccctggac ccttaccagc
    21961 atgaccgggt catccttgcc tgtatatttt ccagcgatat tgtggagtct tgtggtactc
    22021 acaaccgctg ctatgtcccc agtttctctt gaccacactg attctaccac ataccgctcg
    22081 ttatccctga gaagcgctgc aatgtcatat aactcctcag gagcattcag ttcgatcaac
    22141 ctgtcgcctt cagtatagtt aacgtccatg ataacaaact taaagccctt tgccatgttg
    22201 ggggaaagga tcaaccctga attgtacata gggtctgcaa acatcaggta aagaggtagg
    22261 ttataggcgc ccggatctgt tttgtccgca gcgaagaata caaagggctc atttggtctt
    22321 tcctcaaatt ccatttccgc aacagcaggc cccatacctt tcacattacc ggaaaaagaa
    22381 tctttcagaa ggtcctggcc agcaccataa aggccctgat ctttggcaac tgccgtacct
    22441 gcgaggaaag cgtcccaggc cagcttatga agcttctcat agcctgtgcc atggctatgt
    22501 gcaaaaagaa ttgccacatc atcaccggtg tggcaaacaa aaaagtcatt aacaagtcct
    22561 ttggaccctt tttttaagaa atccttcacc cgctgcataa gttgttcact tggctttatg
    22621 tggccgccta tgctcccaat atccgccttg ataatcgata atgttgtttt cataatcccc
    22681 tcctgaaata tttttggaac ctgcttattg acttaatact cgtcacatcc aggcctaatg
    22741 gcgttatgga aacgaaacca tgctcaatgg cataaaaatc agagccctcc atgagttcaa
    22801 actgttcgcc gttgccgcct atccagtagt attttcctcc ccgtgggtct gtccgttcaa
    22861 tcactttatc attatatatc ctttttccaa gtcttgtcac cataaaaccc ttcattttgc
    22921 tgtagggaac attcggaaca ttgatattca ggaaagtcga ttccagaaag ttgttctctt
    22981 ttaccacctc aataattgcc tgtacaatct tgatagcatc tgcaaaataa aagtttcttc
    23041 gtgcatttaa tgaaacagcc aaagagggta taccaagaaa agctccctcc tgtgctgccg
    23101 caacagtccc agagtagttc acatcctggc ccatgtttgg ccctttgtta ataccggaaa
    23161 taataagatc aggtgccctt ttcaaaactg ctttgactcc gagaaacaca caatccacgg
    23221 gtgttccgtt tgtggcaaac atgccccggt ctacctccct aatgcgcaaa ggcttatgaa
    23281 gcgtgattgc atgccccacg catgttctct ccctttcagg agcaactgtg aatacatcgt
    23341 atttttggga cagattattc gcaaggagcg taagcccttc agaatgaata ccatcatcgt
    23401 ttgtcagcag gataagcaac agacacctca ctatcagcgt tcagcctctg gcaaaaaaac
    23461 ttcataacac ggcaggttga tgaaaaagct acagcttttt ttgcaccgat agccgaccgc
    23521 tgtcagcgat tagctctcag ctttcgacgt attggggaac aaaacctact tatgagccgc
    23581 atttgctgaa agccaaaaat aacaaatcgg ggcgactgga tttgaaccag cgaccacttg
    23641 aaccccattc aagtgcgctc ccaaactgcg ctacgccccg actttttaaa tattacagta
    23701 taatcccata aaagtccagc aattataatg gttatgggtt atgaatttta aattttgagg
    23761 tataaaaacc aaaaaatcaa aaacattttt tcgtattcat tttttcttaa cttctttttc
    23821 cctctttact tcccgtccag ttcatcccgc cttaattgtt gacatcactt ccaattgtag
    23881 actacgattt tctcagtgac catggaccat gaagacattc ttctcatcca tatgggaggc
    23941 cttggtgaca tgtgcctgtc tgaatccaca tttctctcgc tctcccatca cttccagaaa
    24001 aatatttcag ccttagggta tttgcgcttc ttcaatttct ttaaggagta ctttaaaacc
    24061 attaacaaca tagagtcagc aaggtggctc tatctgttct cagattatac cacggaacaa
    24121 acctggaaaa ggatcgtttt cgtcggtaag gacaaaaatg gggcgcttcg aaaaagatgg
    24181 aaggcagttt cacgggatga gatgatattt gtggatatgt accctgacaa tacctatggc
    24241 cctgttgatt cggaagccgg gacacttact tcgaagatag aaaactatca gctaaaaaat
    24301 gtgatgcata tcgaaagcta tcaattaacg cagctcgaac aatgtggcat aaagcccctg
    24361 aaaaaaaaca ttaaagcgct acccagaaat cgtgtcatac tttaccctga ggcaagtatc
    24421 acgaaatcca aatggcatta cgaaaacttc attgaactct attataccct caaggggcat
    24481 ggcgttgaga cgcatatctt tgaatccttc ggcctgaacc ttcctatcgc tgagaaaata
    24541 gtgatagaag atttggccga tgttaaaacc ttttttgatt ctggtggtat cttcgtatcc
    24601 aacgactccg gtatggcaca ttttgcaggt ttctgcggac tccttaccat aaccattttc
    24661 agcgattttg attcttccat ctggcatccg agaggggaaa acatctccct ccgtttaggt
    24721 tatgacaatg tggtggcaac agaactggtg gacacaatat accggtgcct ggctgaagaa
    24781 acagaataga aggtgctgat ggttgtggca taatgaaaca acaatgctga cttctgtaca
    24841 ggcgcttaag ataactacaa ttggaggttt caaacatgaa ggacaacgaa caggaaatca
    24901 aagagataat ccaacgttcc ttctctctgg gggttgaata tgaaaggaca attcgaggct
    24961 gttgccagtg tactatagcg gctattcaag atgcccttgg cataagaaat gatgctgtgt
    25021 ttaaagcagg aagtggatta actgcaggcg gcggggtttc atgtgaaggc tcatgtggag
    25081 gctttaccgg cggagttatg gttatgagtt ctgtctttgg ccggcgaagg gataaatggg
    25141 aaaatgacca ggttgataaa gactgtgcac acaacatggc cagggcactt ctcaataaat
    25201 ttaatgagaa gtatggaagc catatctgca ggataataca tcaccgcctc tttgacagag
    25261 agttcgatct caaggatgct cgggaaagag aaatgtttga acggttaggg gctcatgatg
    25321 ataaatgcac ttcagttgta ggcacagcag ctgcctgggc aaccgagctc attcttgaag
    25381 agcttgcaat acgtaaaatg tcgttaaatg atatcaggaa gatgcaggtt tcgggaaatg
    25441 gagtttgggg acgttcttga aatgttgaaa tgtgatgaaa ttgacaaata aatcaagcaa
    25501 gctataatga aaaacagatt cccacgggca agcccatggc gctaaaacat acacacgctg
    25561 cgcgaattat aaacgcattc acccacgctt aaaagcgtgg tcctctgtgg ggcttgcgtt
    25621 tttcgtgaga agctctacgc gagcacagcg agtgtgaagg ggtgtctctg acggcttagc
    25681 cggtagaggg gcaacgcaag ccccagtaat agattatcga gaaaaaagga ggagcggcaa
    25741 tgaaaaagtg tgttgcatgg ttatcgtcag tgattctttg catggcatca cagatcgctt
    25801 tttctgccac atcgaatgat tattttcccc ttaagcctgg aaccgtgtgg gaatatacga
    25861 cagatcaagg aggcactgct gaaagaatag ttctcaagaa aaccgccatg gtaaatggag
    25921 cagaaacgag cgtcgttaaa tatgtagatg aaggattgag aacttacttt tccagcgact
    25981 taagcggtat ttacctccat agagagtatg agcccagtgt ttacattagc ggcgccggcc
    26041 ggaaaagcat tgacgttact tttgatcctc cacttaagtt cggaaatgcc acatttgaga
    26101 ttggtgattc ctttcattcg gagggtacag caataacacg tgcggctgga agaacgttta
    26161 atttcggcta cagagcagac gcaaccgttg aaggcacaga aactgtttca gtgcctgcgg
    26221 gaacctttga taccataagg gttagaatgg aacttaccct tatgtcaggc ggctatggag
    26281 actttacaac tacctcggtc ctaaacttcg caaaggatat cggtgttgtg aaggattttt
    26341 caaccgatac cgataatgta accacaaccg aggaacttat atataccaat gcgaatgttt
    26401 atgatctcgc cataattagc ataacacagc caaaaagagt gactttgtcc cccgcttccc
    26461 caaccaaaac ctcattcctt aaagtaaagc tccagaacaa agggcctttt atcgaaatca
    26521 tcgaggatgt aactatactc acgaacctta tcaccctgac cgcagaatcc ctaggtgcct
    26581 gccccgcgcc tttaccagtc cttcatgcag gcaagcctca gaaacctttc ccaatagcga
    26641 taaaaccggg gaagacactg acggtctatt acaatgtaac ctttgactgc gcaaacaatg
    26701 caaccaagaa taccccggat tttcgcttta gcgcaattgt gaatcgtacc gccattgatg
    26761 acaaggatga taccaatccg gctgataatg cctgtctgag acccggcaac ctgccaaaag
    26821 ataaaggctg cggcgcccga aaacctgatc ataccctggg aggagaaatt ttgactgacg
    26881 tggtcataaa atagcttggc aagccctcaa gctaacctgc tgactagcct tcaagctttc
    26941 agttcaactc gctttttagt ttctaaggtt tttctgtttt ttctggttac ctaaaccagg
    27001 taaatgaatt tctgctgttt atcttttgtt ctcttaatcc cctaatccta taatccggat
    27061 tttctaatcc tgttcttccc atttcgcggt acgcgtttca acctccgcgt tgctggagtt
    27121 gccccctcgc ccttcccgcc tgactttttt tctcctttcc cgtcttcatt tttcaatcct
    27181 tacttctatt cccgcctttt ccgtccttcc cgtctttccc gcatccccta ttgcccattg
    27241 cctcttaccc gttttcaaag aatattgttt tcatgttctt ccggtgataa aattagttta
    27301 tgacctacat cacagatgta tatcttagcg agatcctgaa gaaagatgtg gttgaccaat
    27361 tcggaaggaa agtaggtgcc ttgtgggacc tgaccatagt ccccggtgca gaacttccaa
    27421 gcgtaaccag gttgatctta agaaaaagac gtcaactata tgctgtgccc gttcaatgcc
    27481 tccaacactt caaccgattc gtcataactg tagacctgtc ggatacaagc cccgggtctt
    27541 acagtattaa agagggagat attcttataa gaaaacatat tcttgacaaa caaattgtcg
    27601 atgtaaacgg tgccaaagtc gtgcgggtaa atgatcttaa gcttggtgac gctgacggct
    27661 cggtgtgtgt aataggtatt gatgttggcc ctaacggtat cctgagaaga atagacggag
    27721 gcagcctgat ccagaaagcg ctccagacct ttaaccggcc aatcaaagag tctgtaatcg
    27781 ggtggcacta cttgcaaacc atggatcccc acctccgtca tttgacactg aacgtggcaa
    27841 aaaagcagct cggcgagctc cacccttcgg atttagcaca aatattgaca gaagtcgcac
    27901 ctgacgaggg cactgaaatt cttgcgtctg tcgacgagga actcgctggg gaagcccttc
    27961 atgaggtgcc agatgaggta agggaaaaga tcctcaggga gatggacaag gaactgattt
    28021 ctgatatcct tgaagaaatg ccgcctgatg aggcagcgga tatccttgga ggcatgcctg
    28081 aagaaaaatc agaagaattg ctctccctga tggagaaaga ggatgctgaa gaggttaagg
    28141 accttctaag ttacgaggag gacacagcgg gtggtctcat gacgaccgaa ttcctcgatt
    28201 tcacccctga tatgacaata gaagaagcca tgaccgatat taggctcctc atccctgatg
    28261 tggaatttat ctattacatc tacattgttg ataatgagga tcatctgctc ggggttgtaa
    28321 ctttgaaaaa aattttgaca aatcctctct acaaaacact gcaagaggtt atgacgcaga
    28381 atgtaaaggc tgttcacttc gacaccgggc ggaaagagat tgctgagatg atatcgaaat
    28441 acgatatcat agctttgccc gttgttgatg acgataacag gatactgggc atcataacag
    28501 tggatgacat tatcgatctc ctcgttccaa atcccacgag aaggcgcaag aaacgataaa
    28561 gcccggctat tatcaataac tggttttttt gagttgatcg agaattttga ggagatcctg
    28621 cgcatccctg ctgtttatcc catatctgtt gactgctttc tcaagattgg ttttagcaag
    28681 gtcatacctg cctatctcca aatagtattt gccaagatac tcataaccca taccctccag
    28741 gcccattctt ccagttagca ttccaatcct gtaataaatt tccggaaata ccggcccgta
    28801 gggaagcaaa tctctgtaag tttttgcagc catttcaaca tcgcctaaac cgtcgtatgc
    28861 ctttgcaagg aacatttttg acatgggatc atgtttgtat ttcagtatct caaccgcatc
    28921 tgcaaacttc cgcccattta caagggcctc tcccagaaaa aggtccctgt acggtgattt
    28981 gatattagag gcaatcttca ccgattccga aatgttaccg gcagacatgt aggctaatat
    29041 cgcaccatag gcatttaagg gagaatcctt atccttcttg tacttattta accatatctc
    29101 cttcgatcct gtccctggcg gcctatagag gacattagcc cgtaccacca ggtaaggata
    29161 aaacgtcgta ttatttttag ttttgctatc cgcccaggta ttctctattt ttataattct
    29221 ttcttcatga taggggtggg ttagaaggta ctgggggata gtcacttctc cgcttgatat
    29281 tctcaatctt tttaaaaagt ccgccacacc ggttgcatta tagcctgcct tatccgcaaa
    29341 tatcgctcct gtcctgtctg catcctcttc atcatctctt gaatacttta gagacaatgc
    29401 ctgtgctgct gtctggcttc cagcaataat cgcctgtttt gcctgtgagt caccaccgac
    29461 caatatccct gccagcatag ttgcaagcat gccgatgtta atatactttt ctttctctac
    29521 cctccgtgca acatgtcgtc tggttatgtg ggccaattcg tgtgcaagaa cacctgccag
    29581 ttcctcctct gtttcagaaa aggaaatcag gcctgtagtc atatatatat atccgccaac
    29641 tgttgcaaaa gcattgacag tctgggattg aatcaccgtc aaggtgatgg gaaatggtag
    29701 agtggcaacc tcctcaaggc gcttcttgat atcctgcaga taaagggaga tgaaaggatc
    29761 attattgatc gggacagagt ttgctatctg ataaaatatc tctttgccat attttctttc
    29821 ctcttcaact gtcaatcctg aggcgagcga ggagacgcaa agagagaata agaatagaca
    29881 aacaacaaaa aacagcttta acttcattat cttgctgcgg atttcttact gcgttttttg
    29941 tacttttcag ttttgtattt tgcagttttc ttttttacag tggtcttttt tacagtggtc
    30001 ttttttacag cttctttgcg aggtgttttc tgtttgtatg atgtgagtgt aagagagttt
    30061 gattttgcaa gcctgatttc ccggtctgat ggaagatcag aataaaggct ttgtagaatt
    30121 ttgattgcat ctccccacag atctctgctt cccagcattg ataggatgta aactcttccc
    30181 tctttttcaa aagcacccac ataacaatgc tttgatgccc tggtaaaacc ggttttgcca
    30241 ccaatcgttg gttcaaaaca gaataaaagc ctattgtggt tcttgtatct tatgctcttt
    30301 ttgctgtcat taaaaaggaa atatctggtt gcaataatat ctctgaaatt atcgctctcc
    30361 agggcatatc tgaacatgat agcaagatcc cagcacgttg tgtactgtcc ttcaacgaat
    30421 aggcctgaag cgtttttgaa atttgaatca aacgccccta tagaacgggc tttatcgttc
    30481 attatctttg caaatgattc ctctgagcca cctatatgtg tgccaattgt gtaagcggca
    30541 tcatttgccg actcaatcat tgtacccttt atcagatcaa aggctttgta ctgcctgccc
    30601 ggtataaggt taagttttga tgcagggatt ttcacaacct gcgcattcgg tacaatggtt
    30661 tcattaccct gaaggttctc cagtgcaaca atagtggtca agaccttcgt ggtacttgcc
    30721 ggcggatgtt tatgatcata attccttccc gatatgacct gaaatgtatc tttttcaaca
    30781 agcaggtaac tacaggccgt aataccttca tgtgctgaaa gagaacaagg aatggcaagg
    30841 aacaagcaga ttatgacaat aaaaaaatgc actatgaatg ttttataaac cattcttttt
    30901 ggtttagcaa tataaaattg aaaaaaacgg gcaatgggcg agagattatg gacaatgggc
    30961 aaaaaaaggg gaggcatttg cctcccataa aaaagttttt acatcaacga gttttcaact
    31021 atgggctata cgctgcaaca catttaggcg ctgttagttc aaccactttg aatctttgtc
    31081 aaaaaatatc ttgatattcc tgccaataaa atcaaaaagg gattcatatt gattaacgtc
    31141 ctgtgctaca tcacgggtcc atggagacag cacataggtg tacgacgtct tggaatctga
    31201 tcctctgaaa attcttatgg gaatggctat cgatattccc ttatcgtgat accctctgtt
    31261 taatggatct ttgaggttgg acgtgtctgt aatgctgtac catgctgata ttgttacccc
    31321 cttaataaat ttagatatcg tgaatttcgc acctatatct cctgcaagga atcttcctgc
    31381 cttcatatcg agagttacgt catactcagg aatattgagt cgcgagttaa aaaatgccgt
    31441 tttatatatc tgggtataat gatcactcaa ctttagaaca ttgtccggat ccctttttct
    31501 cacagcgctg cccccaagac caaacattat tctcccatcc acaatagtcg tggcaatctc
    31561 gccatagatg cctgcgtact gcagttccag atatcctgca ctaatccgac tgtacaactg
    31621 atttgttgcc ttgtaggtct gatcaaacat cagccgtgcc agtgtcacat ccttttcctt
    31681 gtacaaggca aaatcactcc tcacagcatc aactaaaggc agggttctgg tagagatatt
    31741 gttaatggga taccactcgg gctgggcaag gaatgttgca cctttccagg gacgatagct
    31801 tccccatgct gctaagccga agcgatactg aaaaaaatcg gctacgtcat aataattctc
    31861 aatagacatt tttgcaccgt aatcaatcag actccggtga aacaccctgg tatcagagcc
    31921 tcccgtaata gacgggtcaa aacttgacaa atagatcagt tcatcgttgc tcagcttctc
    31981 tgtttgccag tcccctatgt ctgcacgcat ggcgctgaat ttgaacaaag gaatgccatt
    32041 atcatttata atgatatgga tattctctac atgacggggc gttatgtcgt taacgagttt
    32101 tagtattatg cctatagctt ttgtaacata gaaatatctg ttattctgtg cttcaacaaa
    32161 cacgtcagtc acattgattt ttacctgaat attgctgaaa cctgatgcaa acagggcatt
    32221 aatgagtcgt gtttccacag aatagccctt gtcatctgct ttctccttgt atgattcatc
    32281 atagatcggg ataaggggct ttcctatttc aaatagggtg gacaggttaa ggctaaactg
    32341 ctccccccgc tggtatgaca catcaacctc cgcccattta aatggtttcc acctgaatcc
    32401 aaagttaaac cctgaaggaa ctgctttatc gaagtatttc ctgcgggcag gatcagaaat
    32461 ctgcttgtgg aacttgatgg gactgtattc catcataaaa gaatacctgt cggaaggaga
    32521 gaactgtact ccccagaaga tttgggattc cttaaaccat cgcttcggat cagtgaagat
    32581 ctctgccttt atatcttcat tggatggtgc aaggggtttt tttccaaatc ttccgttgcc
    32641 aaaaccaatg gtgaaatcaa aggggtagat ttgtttactt gccacgaggt attgggatgg
    32701 atacagacgg gttccctgtg gatccataat tcccaacgca agagctggtc cgtatttact
    32761 ctcagggaga aagcggtatt tgacatcaaa cgccttgtcc ctataccagc cgctagaagg
    32821 tgtctcgaag gctttcactc cgacaacttc cgtcaccctc ccgtcaacct ccaaaccagg
    32881 gaaaagcccc aatgcgccat agtaatagcg gtaaggggag acttcagcaa agccaaaccg
    32941 gtatgtgttc tctttcatta cccgcgcagt tggaatctcc attaatccag ttccgcccca
    33001 atttgccgat ccaagaaatg gctcatcagc agcgtatgca acaggtggga aaaacgggaa
    33061 aggaggtaag gcgggaaagg aggtaaatac gagaagagcg agacaagcca gaagggcgtg
    33121 caggatgtta aagcgcaaaa aacgagccaa acaaaaaaac aagctacgcc ataaactgat
    33181 ttgaaagatt attctaaatg gatataaaat gatatgatga agaagacctg agcctatgtc
    33241 aacacaatga attaatagat acggtaaaat caagtccgtc atccattaat gtcgacaatc
    33301 tcttccttat gtgtactttt cagttttcgt atgaaaccgc cgatctgttt agataacagc
    33361 gcgattcttt catttaaatc taagtatata tcagcattta aatatccgac tccttgagca
    33421 atgtaaagct gggttttcag ttctgcagct gaaccctttg caacgcataa atacctgacc
    33481 aactccttaa tggtctccct ttctttgcct tctgcaatgt tactcggtat tgaaacagat
    33541 gagcgcctga tttgatcttt caagccaaaa tctttggtaa acggctcatt tgtagttagc
    33601 tgatagatat caaccgccaa ccgacaccct tcttgccaga tttgaagctc ttcatagttt
    33661 ttcattgttt tcgcactttg tttctcatag ctgcatcagt ttttcccacc agtctcacct
    33721 tacttccctt ctcgcctttc tcgctttact tcctttcccg ctaatctaac gttttataca
    33781 atacggctat tgttgctgct gctacacctg cattggcaag aatctgggta atgtccttca
    33841 ggttcctgag ccaggccgtt ctctcaagcc tgtctggaac aacaatagag tcccctgcct
    33901 caagcactcc tctggattca ttgaatcccg caatctccca cctctttttc agagggctcc
    33961 agttcatgaa tccccttgaa agctttcttg cgctaccatc aaccttcata acaaatacat
    34021 tgtcttcatc tgcatgccgt gaatagccac cagccatctg gacatagtct tcatagcccc
    34081 atttctcatt atagataaag ctgccattcg acattactgc cccgactaca ttgattacct
    34141 tgttttctgc tggtatataa agggtatcgc cttcttcaag ctcaatgtca tactcactcc
    34201 ccttaaggag cctcaggtgg gcaagcc
//
LOCUS       DAOOMC010000009        33997 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdus sp. isolate AMR_MDS_2685
            NODE_1052_length_33997_cov_6.926463, whole genome shotgun sequence.
ACCESSION   DAOOMC010000009 DAOOMC010000000
VERSION     DAOOMC010000009.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864760
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdus sp. (wastewater metagenome)
  ORGANISM  Syntrophorhabdus sp.
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae; Syntrophorhabdus.
REFERENCE   1  (bases 1 to 33997)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 33997)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,729
            Genes (coding)                    :: 2,708
            CDSs (with protein)               :: 2,708
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 7 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33997
                     /organism="Syntrophorhabdus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1052_length_33997_cov_6.926463"
                     /isolate="AMR_MDS_2685"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2699756"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            367..1005
                     /locus_tag="PK125_01655"
     CDS             367..1005
                     /locus_tag="PK125_01655"
                     /inference="COORDINATES: protein motif:HMM:NF017638.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase 108 family protein"
                     /protein_id="HPB36844.1"
                     /translation="MLSHVNLIEAILASKQLDLSRRAGRLPHTQNVGESFEKVLKNNL
                     QVSSSVLPLNNEIAQGNAKFREGLKFVFEREGSKYVREDGGRESSKYGILQSTAKECG
                     FRGNVRNLTKADAEIIYRKIWDKSGAESLPFPLSTVHFDTYVNSPTAAEKILSKSHGN
                     IDVYLRLREQRYARLADIRPEQYGKYLKGWNNRIKNLRIMVASYNDKSNFTA"
     gene            1321..2949
                     /locus_tag="PK125_01660"
     CDS             1321..2949
                     /locus_tag="PK125_01660"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HPB36845.1"
                     /translation="MKEKIKKDGKFLEALKSIRHQIDTFERSATYRKLAELVLVESEE
                     RYRTLFEAANDAILLIQDDQIIECNAKSLEIFGCNKPQLIKEYPERFLPEYQSDGQLS
                     KLTSEEKIKSALEGVPQFFEWKYRRYDGMLFDAEVSLNCVELSGKRFLQAIVRDITKR
                     KLAEEELKRERQKLQTLLEQAPFGMILVDVKGNYKYMNPRVRELFGYNLNDTPNGRMW
                     LKKAYPEPSYRRTVVAAWKDEIRKINRKKKVPRVFNVTSKDGTEKIVNFRVVQLEKNG
                     GYLVTCEDITDLKRLEDELLNAQKLESVGILAGGIAHDFNNILAVVLGNISLARSHLN
                     NEGIVSDRLLEAEKACLRAKDLTQQLLTFSRGGESVKEPIDLKDIIREAVNSVITNSQ
                     FECNFSFADQLFPVLANEREIRQVFSSILYNARESMPLGGAVSISVENVTENPKDELP
                     FLERDYVRVSIKDTGSGIPQSQLQRIFEPYFSTKPLGTQKGMGLGLAICYSIVKKHHG
                     FIEVESNVDSGTKVNVYLPAARNDRDMSTGPTGI"
     gene            3033..3407
                     /locus_tag="PK125_01665"
     CDS             3033..3407
                     /locus_tag="PK125_01665"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HPB36846.1"
                     /translation="MKVLVVDDFATMRKIVKNVLKQINIENVVEAENGKHALNVLNSE
                     EIDLIISDWMMPEMTGIEFLKVCKEDDEKKKIPFIMVTAEGQKGSVMEAIKSGVDNYI
                     VKPFTPDKLKDAIDRARTRAGK"
     gene            3424..3897
                     /locus_tag="PK125_01670"
     CDS             3424..3897
                     /locus_tag="PK125_01670"
                     /inference="COORDINATES: protein motif:HMM:NF025070.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheX"
                     /protein_id="HPB36847.1"
                     /translation="MEVKYINPFINAAQTVFKTMLNIDAKMGKPILKNEKTTSGDISG
                     IMGLAGDRKGTICISFHDKGALFVFKTLVGEECESINPEVVDSIGELTNIISGQARKE
                     FEQTGLNLKAAIPMVVVGKNVEVNFITKIPIISLPFYFSINNGTQEIMYLDFSFE"
     gene            4300..5736
                     /locus_tag="PK125_01675"
     CDS             4300..5736
                     /locus_tag="PK125_01675"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HPB36848.1"
                     /translation="MEKGKIIIVEDEESILTMLSEFLSNNGYIVDPFIDSKKALFALK
                     GNEYDVMITDLSMPHIDGLQLINYIQREYLKTLGIVITGYGSLDTAISAMRWGAFDYI
                     LKPFKFEEVLTVVESAMYYSKLMKSENIPKGLTGKANLLRRFSENKIIRENTMLRNCL
                     KDKYKFENIIGISFPMQKVFEMIEKVADTNATVLVTGESGVGKELVARAIHYNSSRKD
                     NPLVVVNCGAIPETLLESELFGYEKGAFTGATGTRYGRFELAHGGSMFLDEIGDMSFN
                     LQVKLLRVLQEKTFERVGGTKSISVDVRIIAATNRILDDLVKDGKFREDLYYRLNVVP
                     IQLPPLRERRQDIPLLLNYFLERSNKINDAEIDGCSEAAMTALMDYTYPGNVRELQNL
                     IERVVVLKRKGMIDLEDLPDKIYLAEQQEQGHFEIEKGYDTLVSNFEKSLILQALQET
                     NGVKSKAAQVLSLNRTTLIEKMKRLGID"
     gene            5946..7040
                     /locus_tag="PK125_01680"
     CDS             5946..7040
                     /locus_tag="PK125_01680"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPB36849.1"
                     /translation="MPAVEAKLLENAFQEFAKASDSIINYYSLLESQIRQLKKEVAEK
                     NAELEKARGYLYNILNSLPIGVVVKNTSHLIFVNKTAEVLGPEVFLNKLTDNGQKSGA
                     LKNGRGHYRWKRETIVNGSGSNEIVVFEDVTEFEKMKERLEREERLMAMGEMAARMAH
                     EIKNPLGSMELFLSMLLASKLRKKDKKYIDYVLFGVKTIDRIINNLLSYTKPKTLAVT
                     ESRLSQVVKDTLDFMGLSAHSRGITFEINTCFSEPSFFDPDLIRLVIMNFISNAIEAM
                     NGNQGIIKVDVREEGRYALIIINDNGIGMSEELQKNIFNPFFTTKDKGVGLGLFIVYN
                     IIKAHGGYIEVESAEGLGTSFGIYIPKDGQ"
     gene            7037..8326
                     /locus_tag="PK125_01685"
     CDS             7037..8326
                     /locus_tag="PK125_01685"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HPB36850.1"
                     /translation="MKTNVLVVDDDDHMRIALKESLSKIGYTVSLADNGLKAVEEIDR
                     NMFDLVITDVKMPHLSGIDLLQRVKDKRPFLPVILITAYGTVQDAVKVIKEGAFDYIQ
                     KPFNTDTLYGVVKRALGVNGGKIIYASREMKDVLQSTERVAKTDATVLVLGESGVGKE
                     LVARYVHENSERVDKPFIAVNCAALPENLLESELFGYEKGAFTGASSRKFGKFELADK
                     GTILLDEVTEMDFRLQAKLLRVLQEREVEVVGAKFPKKVDVRIIATTNRNLAQYVEEG
                     KFREDLYYRLNVFPIHVPPLRDRKEDIPPLVAYFLKKHSKGMDVRIEEGAMELLKQKS
                     WKGNVRELENTIARACILSNYSLIKTAHLPDMNSVKDVSVGSVREMEMKLIIDALKSV
                     GGNRTKAANLLGITARTLRNKLKDYRDLGLNIPDKEV"
     gene            8326..8664
                     /gene="flgB"
                     /locus_tag="PK125_01690"
     CDS             8326..8664
                     /gene="flgB"
                     /locus_tag="PK125_01690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0009429 - bacterial-type flagellum basal
                     body, proximal rod [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgB"
                     /protein_id="HPB36851.1"
                     /translation="MEVTAILEKALNIRAQYHKVLSGNIANVETPNYKEKDIDFQAEI
                     QRNIGGSNHVEIRESSDGDVIGSIDGNTVNMENQIVKMTENHMLFTSLVQVISKKFSM
                     MRYIISEGRR"
     gene            8666..9103
                     /gene="flgC"
                     /locus_tag="PK125_01695"
     CDS             8666..9103
                     /gene="flgC"
                     /locus_tag="PK125_01695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
                     /protein_id="HPB36852.1"
                     /translation="MGGILDILKISASALKAERTRMEITSSNLANIHTTRTEEGGPYK
                     KKEVVFIASDISEGEGFGRMLSDRVSSGVKVAEIKESTRDFEKVHDPGHPDADKEGYV
                     TYPNVNLMEEMTDMIAATRAYEANINVINTTKQMFLKALEIGK"
     gene            9112..9402
                     /gene="fliE"
                     /locus_tag="PK125_01700"
     CDS             9112..9402
                     /gene="fliE"
                     /locus_tag="PK125_01700"
                     /inference="COORDINATES: protein motif:HMM:NF014143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HPB36853.1"
                     /translation="MKIDSIAGIGNMVENKGVNAHKGKTTFSEVLKDSIQKAGELEKE
                     ADQEAIKLAKMETQDIHNTMIAIEKADLTFQLMMQVRNKIISAYEEIMRMQV"
     gene            9406..10995
                     /gene="fliF"
                     /locus_tag="PK125_01705"
     CDS             9406..10995
                     /gene="fliF"
                     /locus_tag="PK125_01705"
                     /inference="COORDINATES: protein motif:HMM:TIGR00206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009431 - bacterial-type flagellum basal
                     body, MS ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body MS-ring/collar protein
                     FliF"
                     /protein_id="HPB36854.1"
                     /translation="MDAFIKQVKKYIQTTPKNRLYLYLFLLVAIIGGSVLGLSFIQKE
                     NYNTLFSGLSTEDASAVVAKLKEQKIPYKLGLGGTAIYVPNEKVYDVRLLLASQNALP
                     GGGGVGFELFDKTNYGMTEFMQNINYKRAVQGELARTINQMPEVKASRVHIAIPEKTL
                     FSDREKEVTASVFLKLKPGKNLSKDQVSGIVHLASGSIEGLKPENVSVIDSSGRILYK
                     NGDTNSPMALSGQQYELQRGVERRLEESIQSMLDKFIPASQSIVRANVELNLRKIEKM
                     EEEYQPNKSVIAAEKKSRERTSNQTAKVGGVPGVASNIPTTGGTRDVRPETPNRVNES
                     EREESQITYEVSKSVSKIIEPFGDIKKVSLAIVVDGKYEKVKGQKGEETKYVPRSAKE
                     LNDIKNLVARAVGYDEVRGDKIEVLNIPFEVESSADEKGIIEQAEKKELIFNVSKYVF
                     YLVIFAAVFFFVIRPLLNILQKRGPVLPLKKVRDVYVKGPGADGATAAIEDKAQSAIS
                     DALKDKALVGTIIKEWVKEGT"
     gene            10995..11993
                     /gene="fliG"
                     /locus_tag="PK125_01710"
     CDS             10995..11993
                     /gene="fliG"
                     /locus_tag="PK125_01710"
                     /inference="COORDINATES: protein motif:HMM:TIGR00207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009433 - bacterial-type flagellum basal
                     body, C ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HPB36855.1"
                     /translation="MEAQDIPGIKKAAILLLTMDEEISKEVIKDLEEEDIEAIGKEIA
                     QLKIIPDNAVATVRDEFMRRLSKRGNRVVGGENKFKELIKKSFSNEQAEQFLENMETK
                     KGLPGDFLRRCDPKVLANIMKGEHPQTIALVLSTLGNKKAGEAIVALPEKVQSDVIVR
                     MAFLEKVDTKVLEEIENVLREQLESIGVVEGRQLGGVEMVAGILNQMDRTHESELLEK
                     LEEIEPELAERIKQLMFTFEDLVQLDDKSVQALLKEISSDEISVALKGASDMMKDKIF
                     SNMSERAAAMLKEDIEAMGPIRLSDVEKAQTKIAMIAKKLESEGKIVLSRGNEKFV"
     gene            11977..12594
                     /locus_tag="PK125_01715"
     CDS             11977..12594
                     /locus_tag="PK125_01715"
                     /inference="COORDINATES: protein motif:HMM:NF014196.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliH/SctL family protein"
                     /protein_id="HPB36856.1"
                     /translation="MRNLSKGITVYKIDPFAQEESSKQPEFMSIFDDSGKKAKNDDKP
                     NSEEQARKIFEDAFVQGEKAGYEMGMKKIEQIAKRLNSYITELDVFKDKLLKQAESFS
                     MELALIFAEALVLRECSEKKGLVMDMAKRAFEICEDKHNIVIRVRPEDAEYLSKSTTG
                     HLKIVPDDTLKEPGFIVETEFGDIDGRLSVQLEELRKEFLNVQPE"
     gene            12578..13903
                     /locus_tag="PK125_01720"
     CDS             12578..13903
                     /locus_tag="PK125_01720"
                     /inference="COORDINATES: protein motif:HMM:TIGR01026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliI/YscN family ATPase"
                     /protein_id="HPB36857.1"
                     /translation="MYSLNEDQLSITKKRLSDFYPYKVYGKVNQVVGLVIEGKGPISS
                     VGDAALIFPVDGSDPIDAEVVGFKDGKTLLMPLGDLRGVGIGSKILSKKQAVNTAVGE
                     GLLGRVIDGLGNPMDGGGPIDCRELIPIYRDTINPMNKKRITEPLDLGIRSLNGLLTC
                     GKGQRIGIFAGSGVGKSVLLGMIARHTEANVNVIGLIGERGREVREFIERDLGDGLEH
                     SVVIVATSDQPPLIRVRGAFLTIAIAEYFRDQGNDVLLLMDSLTRFGMAQREVGLAIG
                     EPPTSKGYTPSVFSLLTKVLERAGNGEGEGTMTGIYTVLVEGDDLDDPIADAARSILD
                     GHVVLSRKLADTNHYPPVDVLRSISRTMKDIVSKEQIEYASKIIEVLSEFERAEDLIN
                     IGAYTPGSNKKIDYAISMIDKMKLFLTQGIDEKTTIEETFNSMKILFYV"
     gene            13943..14119
                     /locus_tag="PK125_01725"
     CDS             13943..14119
                     /locus_tag="PK125_01725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB36858.1"
                     /translation="MMDSKNKKKIVKQDMDDPIKPDLSKSGLPVAYQQLLSLIFESPD
                     PLIYKGFFHDKISS"
     gene            14100..14528
                     /gene="fliJ"
                     /locus_tag="PK125_01730"
     CDS             14100..14528
                     /gene="fliJ"
                     /locus_tag="PK125_01730"
                     /inference="COORDINATES: protein motif:HMM:NF014144.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export protein FliJ"
                     /protein_id="HPB36859.1"
                     /translation="MTKFRLSRIIDVKEKLIEDKERELEEAINTIDDITLALDATEKD
                     VEKTYNELAIPSLSGGDFSVLKDYLSYLNDRKQRLIDEKDLTQQRIEQLRINLINLMK
                     ELKMLEILRSKAAKVVKRTENRRIQKNLDSMALRIGERRI"
     gene            14777..16330
                     /locus_tag="PK125_01735"
     CDS             14777..16330
                     /locus_tag="PK125_01735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB36860.1"
                     /translation="MDSLLINLIGIGAHGGPASNASPKEDSGTEFNQKSFFEVLKAAI
                     SEGSSKTSFLNKLDEIGGSGESNLANVMSSISNMLLSMIPPAMVPSVPASETLDAGGQ
                     ETSEMVEDAMDTGQEGLSSAGKQIKVKELCSLLEHANLSALQEGLSDEKPDTIDHETI
                     NRISAGIVKEGETETEEEQFMAFQEEMIKLLSFLKGKPELSGENNKSSAQGELGKAIV
                     QSTPIINLQQTALKVDSNTDSTVTQAQNIPIISELPIEEMMEKNISFLQANVQNRGKF
                     DQDDGKKLDNNRNNEGMDGIFEPDQAFAKTTVKQSFVTEYGLNEEKGRETKGEIHTLF
                     LNAAKKYKEHVSETNESNAVSDSDMANMSPQKPIIEVSQSDQFRANILNIKDNNITFE
                     KGSFTSFMTDRIEKIVEQYSNRGSQMDMVVRLKLDDKETLLIGLKHEGQKIIVDVKAS
                     NDSLVNLIQIHKDDIARHLEGKNIFTNIFVQPDGERNTSKQHDRENKKGERRQETRAS
                     FINILEATV"
     gene            16345..16953
                     /locus_tag="PK125_01740"
     CDS             16345..16953
                     /locus_tag="PK125_01740"
                     /inference="COORDINATES: protein motif:HMM:NF025230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook capping FlgD N-terminal
                     domain-containing protein"
                     /protein_id="HPB36861.1"
                     /translation="MGIQSVGSSYTTTTTQSASNSGNKLGKDAFLQILVSQLKYQDPM
                     SPMKADEMLSQLSQLTQVEQLTNLTASMNAMRKAGDVSQWISTIGKKVNVSTNALSKG
                     DELTLMPQNDYDRIVVTLKNLKDGSSRDITINKGDLLTYTHDGDDMVQVSVKAMKDNK
                     QVSCQAVVFKTVQGVIVADSGPVLVFGNDETYDASQVQVIKK"
     gene            16963..18237
                     /locus_tag="PK125_01745"
     CDS             16963..18237
                     /locus_tag="PK125_01745"
                     /inference="COORDINATES: protein motif:HMM:TIGR03506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook protein FlgE"
                     /protein_id="HPB36862.1"
                     /translation="MLSSFFSGISGLMANSYGINVVGNNIANVNTIGYKGARATFQDV
                     LYQSVFGTAGTSQIGRGTALSSVDTIFAQGSFESTSEPTDLAIGGKGFFVVRQLNGDD
                     PATDQTNLLYYTRAGQFRFDEQGYLVNPAGFLVQGWPIDRVTHERVGVYGDILVSPQP
                     SEPRETSFIAMALNLQANASILPAFDPSDLAGTSNYSSSVTVYDSIGQSHVVSVYFRK
                     VSDGTWEWHARDADSLSTEPDAGSGTLNFTTTGVLDAGSGAPANITLTFPTGTQTVQL
                     GFDGSLGGNPTTQYPMASATNFQTQDGYPPGVLLNVTVSPEGVISGHYSNGQILDLYQ
                     LTLANFNNPWGLTREGGNLFSETLESGVPYTNPPGVNGLGKINPNSLEQSNVDLATEF
                     VKMIVSQRGFQANSRVITTTDEILAELMNIKR"
     gene            18512..19027
                     /locus_tag="PK125_01750"
     CDS             18512..19027
                     /locus_tag="PK125_01750"
                     /inference="COORDINATES: protein motif:HMM:NF015693.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated FliL family
                     protein"
                     /protein_id="HPB36863.1"
                     /translation="MADDEQMVMETEDSEGTPQKKGGKLKLIMIIVLIVLVGGGAGVY
                     FVFGNQLKARFFGGIEQPVKDEKKRESIGPILSLEPFIFNIGGASGKFAKITLGVEVK
                     DAKVMEEAKKMVPAIRDRTLSVLGSKGPEVLMDINNRNHLKKELLNAMKTLFKDDAEL
                     KSLYITDIIVQ"
     gene            19037..20041
                     /gene="fliM"
                     /locus_tag="PK125_01755"
     CDS             19037..20041
                     /gene="fliM"
                     /locus_tag="PK125_01755"
                     /inference="COORDINATES: protein motif:HMM:TIGR01397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliM"
                     /protein_id="HPB36864.1"
                     /translation="MEQILSQDEIDALLKGISEGEIETQKEDYELAEVAENTVQEKIE
                     VRTFDFLKYARSKKERLPALEFIYDRFSKSFRSALSLFVEKEVEVNLEPMQYVSYGDF
                     IETLPLPTNMNVVVTEKLKGFFIVIFDTKLIFSVLETIFGSANTSKKQKIEGREFTKI
                     EFNVIKKLINIVCAEMEKAWAPVHEISCKYSRSEINPNYITMIVSEETVNVCELTIEV
                     EGIKGWMKICIPYGILETIKGHLISTPSHEDSEMREKWIMKIKERINDVPLELKAILG
                     KKKMALQEFLKLSEDNVIIIDKYVNDPVEIEICNRSRIRGKLGIYKGNKAVRVEEIIR
                     "
     gene            20118..20429
                     /gene="fliN"
                     /locus_tag="PK125_01760"
     CDS             20118..20429
                     /gene="fliN"
                     /locus_tag="PK125_01760"
                     /inference="COORDINATES: protein motif:HMM:TIGR02480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliN"
                     /protein_id="HPB36865.1"
                     /translation="MEEFVTENKTVSSAKKIELNIDNLLDISVEISVEIGRTKMSIGE
                     LLAMSKGSIIELNKLAGESVEVYVNEKLLGKGEIVVANERLGVRVTEIVTPKEMVQKL
                     G"
     gene            20450..20704
                     /locus_tag="PK125_01765"
     CDS             20450..20704
                     /locus_tag="PK125_01765"
                     /inference="COORDINATES: protein motif:HMM:NF016250.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliO"
                     /protein_id="HPB36866.1"
                     /translation="MRVILVLIGIVAGMVFLYRLASKYNFNFKPKSTTEYGLKKMDTI
                     HLGYKKFISVVEVKDHVLVLGVGEKEMSLLTKWKKEDKVT"
     gene            20701..21462
                     /gene="fliP"
                     /locus_tag="PK125_01770"
     CDS             20701..21462
                     /gene="fliP"
                     /locus_tag="PK125_01770"
                     /inference="COORDINATES: protein motif:HMM:NF009438.0"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HPB36867.1"
                     /translation="MKLFLIVMIVCTLIILPAFASAKAVESKGRTESKIDIMNSFTGS
                     EGSKNLISVVLLLTVLAFAPAILLLMSSFTRIVIVLSFLRQAIGIAQIPPNQIIIGLS
                     LFLTFFIMAPTYEKVHSTALAPYMSNKIGYEEFITKSSQEMSTFMLKFTKEKDLALFV
                     GLAGITKPKNASEIPLRVVVPAFAISELKRAFQIGFLLYIPFLVIDMVVASVLLSAGM
                     MMLPPIFISLPFKLMLFVLVDGWNLLIGSIIKGFY"
     gene            21751..22515
                     /gene="fliR"
                     /locus_tag="PK125_01775"
     CDS             21751..22515
                     /gene="fliR"
                     /locus_tag="PK125_01775"
                     /inference="COORDINATES: protein motif:HMM:TIGR01400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliR"
                     /protein_id="HPB36868.1"
                     /translation="MDFTNDIQAFLLIFSRVVSVLWLLPVFSSRAVSVPFKAGVSLLI
                     AYLLFDSIGVDRSFSNDPYLLSLFVMKEVLIGLAIGFSVRILFIAVNIAGEIISIQAG
                     FSFARFMDPLSMTQTTVIEHLKSLLAMMLFLAMDAHHILVKAIVVSFRDVPIGSVTIQ
                     QSFFQYFISATGRIFMTGFKIGAPIIVTLMFVEVALGMLSRMIPQINIFIEGVPLKIL
                     VTVTMFSLSLGVIVPVIVNMFKGMDGEILRLFRLMV"
     gene            22519..23583
                     /gene="flhB"
                     /locus_tag="PK125_01780"
     CDS             22519..23583
                     /gene="flhB"
                     /locus_tag="PK125_01780"
                     /inference="COORDINATES: protein motif:HMM:TIGR00328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhB"
                     /protein_id="HPB36869.1"
                     /translation="MAETFQEKTEQPTEKRLEEAKKKGQIAQSRELPLCLMFLFTAIF
                     LYFMASHGFHQIFKVYVNYISNINLDVNSANIFSILSFGAYRWAWIMMPFFALLIVLS
                     LSGTILQTGFMWSAEAMQFKLEKLNPLNGIKRLFSKRSAIEVLKAIVKITVLAYIAYS
                     IIMHELPSILSLPGKDPGMIISYLGSTCFYLAIKVGIIFLFIGALDFLFQKWQHKKDL
                     MMTHQEVKEEFKEREGNPLVKSRIRSLQREMARRRMIEDVKTADVIITNPTHFAIGLK
                     YQFGIMPAPKIVAKGAGFVAEKIKEVARLHDVPMMENKPLAQALFYAVKVGDYIPEQF
                     YIIVAELLAEVYRQRNRSRI"
     gene            23583..25640
                     /gene="flhA"
                     /locus_tag="PK125_01785"
     CDS             23583..25640
                     /gene="flhA"
                     /locus_tag="PK125_01785"
                     /inference="COORDINATES: protein motif:HMM:TIGR01398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
                     /protein_id="HPB36870.1"
                     /translation="MGRFGSGIKEKSDIFVAVSVVFVILIMIVPLNAFFMDIFLTLSI
                     SLSILILFIGMYINKPLDFSVFPSVLLIVTLFRLSLNIATTRLVLVHGDEGAHAAGYI
                     IKGFGTFIVGGNYVVGAVVFFILTIINFVVITKGAGRVAEVAARFTLDAMPGKQMSID
                     ADLNAGLIDDAGARKRRESVEMEADFYGAMDGASKFVRGDAIAGIIIIFVNIIGGLVI
                     GIVQKGMSFNDALTVYTILTIGDGLVSQIPALLTSTAAGIIVTRAASETNLGSAMITQ
                     LFTQPRAMLLASIVILCIGFVPGIPLVPFLVLSSCTFGTSYLVGKTKKEEIVSVPEVT
                     GEERDKGEFIQPMEILEIEIGYGLIPIVDAEQSGELLDKIRAIRKQMAIELGIVVPPM
                     KLRDNLQLKAGEYLILLKGIEMGRGELMAGYVLTMGHEDKKKTIDGIPTKEPVFNLDA
                     YWIKEKDRDQYVAAGMTVVDHPTIIATHLTEIIRNNAHELMTRQETQKLIDTVSATHS
                     KVIEEIAQNQINAGIIQKVLQSLLREQVSIRDLITILEAVADASSTSREPELITEFVR
                     QRMARSILKPYLIDGTLNLLILEKNLEEKLINSLQPSDQGTYLALDLAFSQQLIEKVG
                     NEAKKAMLQNIQPVILVHPVLRSKLRRFLERYIQGITVISHNEIPPQIKIQSLGVIKV
                     NES"
     gene            25630..26691
                     /locus_tag="PK125_01790"
     CDS             25630..26691
                     /locus_tag="PK125_01790"
                     /inference="COORDINATES: protein motif:HMM:NF012662.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB36871.1"
                     /translation="MKVKTYVFGDVKTGMEAIKAQHGSDTIIVDIKNNGNNTSPKSCE
                     ISIAVDEDLRPDQCDLIDVRKKTEAIWNYALMLMNERLSGIENDIIRERTREYPLPLR
                     ILFDKLIKNGFERKVAMSLISEVYGEIGDRAQDSMKANTSMRRAIAQRISISNITEES
                     GPVLMLGPTGSGKTQTTKKLAKIFAASDKAISIIAYDPFTKGSYDDLMSFSESHGIPF
                     SFTTNGDDISFIVERDKRQKIIDVGGHPNIQRRVLEKIRDINKIIVLPAGARDEKMQA
                     YCNEFGDDTNSTIGFTKLDEEENLGHIGHNLIKLGYPLSLLTIGIGIDDIVIPDNESL
                     FKILLEGNIWKKEERRPLP"
     gene            26658..27401
                     /locus_tag="PK125_01795"
     CDS             26658..27401
                     /locus_tag="PK125_01795"
                     /inference="COORDINATES: protein motif:HMM:NF013793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MinD/ParA family protein"
                     /protein_id="HPB36872.1"
                     /translation="MEKRRKKTITITSGKGGVGKSSIVSNMAYVLGGMKASTYILDAD
                     LSLGNIDIMFGMAPQFNIKDLINGRKRIGEIVVEGPCGIRIIPATSGVTEFSHLSLDE
                     RNILLTSFRELPEYDFLLIDTSAGISSNVVYFNAISEDIFIVVTPDPASLTDSYAVIK
                     VLHKNTGRKNFNVIVNMAKDENEALDVFKKITRVTDRFLDVYLDFYGYIPVDKNINVA
                     TRKQKLWVEQFPNTVATKALQKICDRMVA"
     gene            27405..28142
                     /locus_tag="PK125_01800"
     CDS             27405..28142
                     /locus_tag="PK125_01800"
                     /inference="COORDINATES: protein motif:HMM:NF005413.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliA/WhiG family RNA polymerase sigma factor"
                     /protein_id="HPB36873.1"
                     /translation="MWKRAYAKAQEDDREKTIEEFIPIIKNLAYKISRGFEDNCIIDD
                     LISAGVMGLLEAMEKFDVSRGIKLNTFAYLRIRGAMIDELRSRDWFPRSARSKAKKIG
                     EVIRKLECKLGRYPDEEEVAEALNMDPQDYLTMLKDYGNLSILSIEGISEISGEDRDR
                     IIQYVIDESDDPEQYAQFHEIERILAQELERLPERQKLVLTLYYLEDLNMKEIAGTMS
                     ITEARVCQIHSQAIMNLRTSMRKHLKN"
     gene            28076..29098
                     /locus_tag="PK125_01805"
     CDS             28076..29098
                     /locus_tag="PK125_01805"
                     /inference="COORDINATES: protein motif:HMM:NF020252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HPB36874.1"
                     /translation="MPDSFSGDYEPQNIHEETPEELKLVLRLHDNIKGIRMAGLDTTL
                     KKIRKINILPTFPDIVDGIINLIEDPMSSASDLAKNMDPSMVGEVMRVANTAYFGMRS
                     FRKITTIEHAIAIIGYEHLSHILLQMPFLKMVQVNDEAFDRNGYIRHSIACGVMAKIM
                     STTLHIANPHEVYISGIIHDIGIIIIYQHFRHSWNAINSLVCTKNVPRLTAEREVLSV
                     DHGFIGAVLLENWNIPQSITEAVKFHHSPLKTQIFKDNAVITHLANEFSKRIDFNKDF
                     SNFNYFLSRHRDYIDYMVDLGGHFSPSNEVMFFEKIYSSLDGVRRYLHGTMEGEKEVD
                     DTCNCC"
     gene            29076..30104
                     /locus_tag="PK125_01810"
     CDS             29076..30104
                     /locus_tag="PK125_01810"
                     /EC_number="3.1.1.61"
                     /inference="COORDINATES: protein motif:HMM:NF009206.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0008984 - protein-glutamate methylesterase
                     activity [Evidence IEA];
                     GO_function: GO:0050568 - protein-glutamine glutaminase
                     activity [Evidence IEA];
                     GO_process: GO:0006482 - protein demethylation [Evidence
                     IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0018277 - protein deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator protein-glutamate
                     methylesterase"
                     /protein_id="HPB36875.1"
                     /translation="MIRVIVVDDSSFMRSILTRMLEKDKDIKVVETAANGEEAIDKIE
                     RVKPDVVTLDIEMPVMNGIEALTKIMATNPVPVIVLSALTREGAEVTMEALNLGACDF
                     MTKDISDGPLGMASKEAELIGKVKDVAKRKFSFLVRRLELLKRPVLINRDKRSKHAVL
                     SIGASTGGPPALQHILTSFPQNFPVPIVIAQHMPKLFTQSFARRLNGLSQIEVKEAED
                     KEPLRPGLALIAPGDVHMGLKKNGKDITVDFVKDAQYVYRPSVDHLMFSTAQIYDSSA
                     IGVILTGMGNDGLVGLKELKRRNGFVIAQDEETCVVFGMPKAVINANIADVVLPIERI
                     SEEIIRVL"
     gene            30101..31948
                     /locus_tag="PK125_01815"
     CDS             30101..31948
                     /locus_tag="PK125_01815"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HPB36876.1"
                     /translation="MRLKLKKYVADHNKRDRRLEKALYAIRQGDNYEKEKAIEFLIRR
                     PTRKTVELILPLLQEKDTSTRMAVVQVVQKIGHMNVQAIVNLLDMENEDLRVYACEIM
                     ASMKTPQTVPSLIKALSDESENVKNAAVIALGEFNDERAVKALLNMLRHEQWIVFSAI
                     CSLGKIKSPAAALPLLQIFQNGEEELSLAACEALMDFENDDILDKMFHILKGWSKKKR
                     KRYIEMIIQQGNENLFLRLKRSIGQDLFEHLLPYVVFDRAEVVPILKLMIHFKSKQTC
                     DILLETLTKIDPDEPEYGEILGFFASLSHIWKDNINEYVDKGDEYSLAIIKACVQNKI
                     TVPEDVLISLFLTSSAPVKREIARGAGIIVQGRGYDFLKEAIKDPDGHVKSYAVNVIG
                     EMQLKELENEIIDLSKYGFLDVRINAINALIKLNLIEAKKLIDQFVESGSLEDKKVYL
                     SVAKNISGDDNFPHIQKLFLTKDEEIRKAVVTIIGNFIDDERYAAIFQKLLRSDNVPH
                     EALKIIREKRLAQFKPVLYKIFSNMGKSLWTRYYALLALGAFEDPALFELFVKGLDDE
                     SNLIKIGSLKALSDLKDQKAVNYVKPLTKHTDDDVRSTAEFVMNNLSYS"
     gene            31967..32791
                     /locus_tag="PK125_01820"
     CDS             31967..32791
                     /locus_tag="PK125_01820"
                     /inference="COORDINATES: protein motif:HMM:NF013867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008983 - protein-glutamate
                     O-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HPB36877.1"
                     /translation="MTKEEFVLLRDYVYEKSGIHFAENKAYLLESRLSNRLTELGFSN
                     FEDYYYFLKYGGDKVRSEIINLFNAVTTNETSFFRNPPQLDAFKVIVHKCYLQNGIPS
                     MPIRIWCAACSTGEEPYTLVMILLEMMENLKKNIPFSVFGTDISTKALESAKKAHYNM
                     YSIRNMGESIRQKYFVEKDGSFILKENVKKYVKIDFMNLMDTNAYKMYKQMDFIFCRN
                     VLIYFDEKMKKKVIDSLYESLKLRGYLTIGHAESLHNISRAFKPLIFPGTIAYQKG"
     gene            32795..33163
                     /locus_tag="PK125_01825"
     CDS             32795..33163
                     /locus_tag="PK125_01825"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HPB36878.1"
                     /translation="MKKILIVDDSSTIRKLIRYILKKQEYRITEAKDGMDAMEKLASL
                     EADLIIVDLNMPNMDGIQFVKNLRSNYYYMDTPVIMLTTTKDAELKRDALLAGVNLFL
                     NKPVQPNFLIYKVEGLLKGE"
     gene            33166..>33997
                     /locus_tag="PK125_01830"
     CDS             33166..>33997
                     /locus_tag="PK125_01830"
                     /inference="COORDINATES: protein motif:HMM:NF013767.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HPB36879.1"
                     /translation="MDEKMSQNEEMKEIIEEFIVESNELIEKAVEDVVRIERNPDDEL
                     INGIFRAVHTIKGTSSFLGFNMLSNLSHKAEDVLGRIRKKDMEPDADIADTLLEALDF
                     MKLMIDDIRVKGTESRDVSHTLRKLEFLNKADTRKKIGEILIDEGVISNREREELLEK
                     QKQDGKKLGEIAIEEKLITEKQLSNVLAKQQAHKEEQTIRIDVRKLDELMNLVGELVL
                     GKNRLILLDSLARKGSNNNAMLDTLSDVTNYVEAVTNDLQLAVMRARLVPIGKLFNKI
                     P"
BASE COUNT        10797 a         6180 c         7853 g         9167 t
ORIGIN      
        1 ctggctgtta accagtaggt tgcaagttcg agtcttgccc ggggagccag atatttccgg
       61 gcgcttcacg cgaagcgcct ttttcaatta acagggatca cgccgttccc ttaacatgac
      121 aaacttactg agcttcccct tgcctttctg tgcaagctga atcacttaac cctccatcca
      181 tgctgttttt gttaatcttg cctcgctcat tgttaaacca gttcttctat tatggtctat
      241 cccctcacac acaagaaggg tatggttaat tcatagccct ttacacttcg atcaataaag
      301 ccatggcatt gttgttgcac aatatcaagc gttgatagag ttcatgtaaa tccaatggag
      361 gaaaagatgt tgtctcacgt gaacctcatt gaggccatac tggcttcaaa acagcttgat
      421 ttgagcagaa gagcgggaag attaccacac actcaaaatg tgggagaatc ttttgaaaag
      481 gtcctgaaaa ataatcttca ggtgagctca tctgttttgc cgcttaacaa tgagattgct
      541 cagggtaatg caaaatttcg ggaaggactt aagtttgttt ttgagcgaga gggtagtaag
      601 tacgttcgtg aggatggagg cagggagtct tcaaaatatg ggattctgca atcaacagca
      661 aaagagtgtg gttttagggg taatgtaagg aatctcacaa aagctgatgc agaaataata
      721 taccgaaaaa tatgggacaa gtcaggtgct gaatcattgc cttttccatt gagtacggtc
      781 cattttgaca cgtatgtcaa tagccccact gcagctgaaa aaattctttc aaagtctcat
      841 ggtaatatcg atgtctattt gagattgaga gagcagcgct atgcccggct tgcagacata
      901 aggcccgaac aatatggcaa atatctcaaa ggatggaaca atcggattaa aaatttgcgg
      961 ataatggttg cttcgtataa tgataagtcc aattttacgg cataacattg caccatgttt
     1021 gttcattgac atgatgacag gtctttgacg gaaccgtttc actcatgctc taaaacgtgg
     1081 ttcactatgt ggcttgcgtc ttttgtgggg atatgcacag agcgaattag tatgatcttg
     1141 aaggggagaa tcacacagtt ttgcagggtg agggtgcgat gcaatcccca gtcatagagc
     1201 acgataatat ctttgagaat tcatgaaatg ctgagctgtg tgtatggaac cgttttgatt
     1261 tgtatcattg aacagttatt atcgaaggcc gtacaatgaa tgtgaaagtg attccttagc
     1321 atgaaagaga aaataaaaaa agatggaaaa ttccttgaag ccttgaagtc gattcgccat
     1381 cagattgata cctttgagag atctgcaaca tatcggaaat tggcagagct tgtccttgta
     1441 gagagtgagg aacgataccg gactcttttt gaggcagcca acgatgcaat cctattgatc
     1501 caggatgatc aaattataga gtgtaatgcc aaatcactgg agatatttgg atgtaataag
     1561 ccgcaattga tcaaggaata ccctgaaaga ttcttgccgg aatatcaatc tgatgggcag
     1621 ctctcaaagc ttacttctga ggaaaaaata aaatcagcac tggaaggagt cccacagttt
     1681 tttgaatgga agtatcgtcg ttatgacggt atgctttttg atgctgaggt aagcctgaac
     1741 tgcgttgaat tatcaggaaa aagattctta caggctattg taagagatat aacaaagcgg
     1801 aaattagccg aggaggagtt gaaaagggag cgtcagaagc tccagactct gcttgaacag
     1861 gctcctttcg gtatgatact cgtggatgtg aagggtaatt ataaatatat gaacccaaga
     1921 gtcagggagc tttttgggta caacctgaat gatacaccaa atggaagaat gtggttaaaa
     1981 aaagcttatc ctgaaccatc atatcggaga acggtagttg ctgcctggaa agatgagatc
     2041 agaaagataa acagaaaaaa gaaggttccc agggtcttta atgtaacatc caaagacgga
     2101 accgagaaaa tagttaattt tagggttgta cagcttgaaa aaaatggtgg ataccttgtc
     2161 acatgtgagg atatcactga tttaaagcgc cttgaagatg aacttctcaa tgcgcagaaa
     2221 cttgaatcgg ttggaatcct tgcagggggc atagcccatg atttcaataa tattcttgca
     2281 gtcgttttgg gaaatatctc tcttgcccga tctcatttaa ataatgaagg tattgtaagc
     2341 gataggttgc tggaagctga aaaagcttgt ctgagggcca aggacctgac tcagcaatta
     2401 ttaacgttct cccgtggagg cgaatcggtg aaagaaccga ttgatcttaa ggatattata
     2461 cgtgaagctg tgaacagcgt catcactaac tctcagtttg aatgtaattt ctcatttgca
     2521 gatcaattat ttcctgtcct agcaaatgaa agggagattc gccaagtttt cagcagcatt
     2581 ttatataatg ccagagaatc aatgccctta ggtggtgcag tcagtatttc agttgaaaat
     2641 gtgacagaaa atccaaaaga cgaactgcct tttctcgaaa gagattatgt tcgagtttcg
     2701 ataaaagata ctggatcagg tatcccgcaa agccagttgc aaagaatatt tgaaccttac
     2761 ttttcaacaa aaccattggg aacacagaaa ggtatgggcc ttggacttgc tatatgttat
     2821 tctatcgtta aaaaacatca tggttttatc gaggtagaat caaatgtaga ttcaggaacc
     2881 aaggtgaatg tctatctgcc ggctgcaaga aatgaccgtg atatgtcgac gggtccgacc
     2941 ggaatttaat tcagaattgt taagtttatt attttttcaa cgattaataa tttagtaatc
     3001 atactaaaac gtagtcaatg tggaggtaaa caatgaaagt tttagtggtt gatgattttg
     3061 caaccatgag gaagattgtc aagaatgtgt tgaaacagat aaatattgaa aatgtggtag
     3121 aagcagaaaa tggaaagcat gctctgaacg tcctgaatag cgaagaaatc gatctcatca
     3181 tcagtgattg gatgatgccg gagatgaccg ggatagagtt ccttaaagtg tgtaaagagg
     3241 atgatgaaaa gaaaaaaatc cctttcatca tggtgacggc tgaaggtcaa aaaggcagtg
     3301 tcatggaagc aattaaatcg ggtgttgaca actatattgt aaaacctttt acaccggata
     3361 agcttaagga tgccattgat cgagctcgga ctcgggcagg aaaatgaacc attaggagga
     3421 gagatggagg tcaaatatat caaccctttc ataaatgcag cccagacagt ctttaaaacc
     3481 atgttgaaca ttgatgccaa aatgggaaaa ccgattctca agaacgaaaa aaccacttca
     3541 ggagatatta gtggtattat gggcctggca ggagatcgca agggaacgat atgtataagc
     3601 tttcatgata aaggtgcgct ctttgttttc aagaccctcg ttggtgaaga gtgtgaaagt
     3661 ataaacccgg aagtcgtaga ttctatcggt gagcttacga atattatctc tggtcaggca
     3721 agaaaagaat tcgaacagac tgggttgaat ctcaaagcag caatacccat ggtagttgtt
     3781 ggaaaaaatg tggaggtcaa ttttataacg aagattccaa tcatctctct gcctttctac
     3841 ttttccatta ataatggtac acaagagatt atgtacctgg atttttcttt cgaataatca
     3901 gacaaaacaa acagaccgat aatttttagc ctggtcttac aaagtgctta atatagtatc
     3961 ctcaggcttt gtgatatcag gatagcgcat tactcttttc atattccgga tgaagataga
     4021 cagtagttct gactatcaga tgtgggttgc aaatgagtct ttgcgcaaag aaatagcagt
     4081 gttttaaaaa tagttgttga aaagtatgcc tttaaacagt aataattatt aaaaaaaatt
     4141 atgcacagtt tattctcttt agaaagatca tttcacatgc ggggaatcct cgtaatttct
     4201 ctgcacaggt acaagacaga caagaatggt gtaagcgaag cgtttttgta aacagaggaa
     4261 gctggataaa ataatggttt ttaaaaagag ggagtacagt tggaaaaagg caagataata
     4321 attgtagagg atgaggaaag tattctcaca atgctcagtg agttcctgag taataacggc
     4381 tatattgtcg acccttttat tgatagtaaa aaagcgctgt tcgcactcaa aggaaatgaa
     4441 tatgacgtga tgattactga tctgtccatg cctcacatcg acggtcttca gcttatcaat
     4501 tacattcaaa gagaatacct caaaacgctt ggtattgtga ttactggata tgggagtctt
     4561 gatacagcca tcagtgcaat gcgatggggg gcttttgact atattctgaa gccttttaag
     4621 tttgaagagg tgcttactgt tgttgaatct gccatgtatt actctaaact catgaagagt
     4681 gagaatattc caaagggcct cacgggtaag gccaatctat tgaggcggtt ttcagagaac
     4741 aaaataattc gtgaaaacac aatgttgaga aactgcctta aggataaata caaattcgag
     4801 aatatcattg gaataagctt tcccatgcag aaagtttttg agatgatcga gaaggttgct
     4861 gacacgaatg caaccgttct tgttacgggg gagagtgggg taggtaaaga gcttgtagca
     4921 cgggccattc actataactc atcacggaag gacaatccgc ttgttgttgt aaattgcggc
     4981 gccatacctg aaacattgct cgagagtgaa ctatttggat atgaaaaagg cgcatttacc
     5041 ggggcaacag gaacacgcta cggcaggttt gagcttgccc atggaggttc catgtttctc
     5101 gatgaaatcg gagacatgag cttcaacttg caggtaaaac ttctcagggt cctccaggaa
     5161 aaaaccttcg aaagagttgg tggaacaaaa agtatcagtg tagatgtaag aattattgca
     5221 gctacaaacc gtattttgga tgatcttgtc aaggatggca agtttcgaga agacctctat
     5281 tatcgtttaa acgttgttcc cattcaactg ccccccttgc gagaaagacg tcaggacatc
     5341 cctcttcttc tgaactattt tcttgaacgt tccaataaaa tcaatgacgc tgagatagat
     5401 ggatgctctg aagccgcaat gactgcattg atggactata cctacccggg gaatgtcagg
     5461 gagcttcaaa acctcattga gagagtggtg gttctgaaga gaaagggaat gatcgatctg
     5521 gaagaccttc ctgataagat ttatctcgcc gaacaacagg agcaaggtca ttttgaaatc
     5581 gaaaaaggat acgatactct cgtgtcgaat tttgaaaaaa gcttgattct ccaggccttg
     5641 caggagacga acggtgttaa aagcaaagcc gcacaggtgc tgagcctgaa cagaacaacc
     5701 cttatcgaga agatgaaacg cctgggcata gattaagccc ctcctgtcaa aaacctgaca
     5761 tcattttaaa ccagttcctg gttctgagtt cttagttata agtcattacg agtacatata
     5821 ccaataaact tctcatgatt tttatcacaa taaaatgtaa ttatttcaat aggatagcta
     5881 acagctaaca gctattagct attagctatt tttagctggt acatattttg caaaatttgg
     5941 caatcatgcc agccgtagaa gctaaacttc ttgagaatgc cttccaggaa ttcgcaaagg
     6001 catcagattc gattatcaat tactatagtt tgcttgaaag ccagatacga caactcaaaa
     6061 aagaagtggc cgaaaagaat gctgaattgg aaaaagccag gggatatctt tataacatac
     6121 tgaattcctt accaataggt gttgttgtaa aaaacacgtc acaccttatc tttgtcaata
     6181 aaacagctga agtgttaggg ccggaagttt ttttgaataa attaacagat aatggtcaga
     6241 agtcaggcgc tctgaaaaac ggcaggggcc actatcgctg gaagcgtgaa accattgtaa
     6301 atgggtctgg aagtaacgaa attgttgttt ttgaagatgt gaccgaattt gaaaagatga
     6361 aagagagact tgaacgggag gagcggttaa tggccatggg tgaaatggct gccaggatgg
     6421 cccatgagat caagaatccc ttgggaagta tggagctttt cctctcaatg ctgcttgcct
     6481 caaaactcag aaagaaagac aagaaatata ttgattacgt cttatttggt gttaagacca
     6541 tcgaccgaat aatcaataac ctgctttcct atacaaaacc caagacactc gctgtcactg
     6601 agtccagact cagccaggtt gtgaaagata ctcttgattt catgggcctg tctgcccaca
     6661 gcaggggcat tactttcgaa attaacactt gtttcagcga accttctttc tttgatcctg
     6721 atctcatcag gctggtaatc atgaacttca taagcaatgc tattgaggca atgaatggaa
     6781 accaggggat cataaaagta gatgtcagag aggaaggaag gtatgccctc atcataatca
     6841 atgacaacgg cattggcatg agcgaagagc tgcaaaagaa catctttaat ccatttttta
     6901 ccacaaagga caagggcgta ggcctgggtc tgtttattgt ttacaatatc atcaaggcac
     6961 atggtggcta tattgaagtc gaatccgctg agggacttgg tacttcattc ggtatctata
     7021 ttccaaagga tggtcaatga aaacaaatgt actagtagtt gatgatgatg atcatatgag
     7081 gattgccctg aaagaatcat tgtcaaaaat tggatatact gtttctttgg ctgacaatgg
     7141 cttgaaagca gttgaagaga ttgatcggaa catgtttgac cttgtcatta ccgatgtgaa
     7201 gatgccccat ctcagcggta tagatctttt gcaacgagtc aaggacaaaa ggccttttct
     7261 tcccgtcata cttataacag catatggaac tgttcaggat gctgtaaagg tcatcaagga
     7321 aggtgctttt gattacatcc agaaaccttt caatactgat acattgtatg gtgttgttaa
     7381 acgagctctc ggagtgaacg gtggaaagat catatacgct tcacgggaga tgaaggatgt
     7441 ccttcagtcg acggaaaggg ttgccaagac agatgctact gtccttgtcc tcggagaaag
     7501 cggggtaggt aaagagcttg ttgcccgtta cgttcatgag aacagtgaac gggtggacaa
     7561 gccgtttatc gctgttaatt gtgcagctct tcctgaaaat ctcctcgaaa gtgagctttt
     7621 tgggtatgaa aagggagctt ttacaggagc atcttcacga aagttcggta agtttgaact
     7681 tgctgacaag ggtaccatcc tactcgatga ggttacagag atggacttcc ggcttcaagc
     7741 aaagcttctc agggtccttc aagagaggga agtagaggtg gtgggagcaa aatttcccaa
     7801 gaaggttgat gtgcgcatta ttgccaccac aaacagaaat ttggctcagt atgttgaaga
     7861 gggcaaattt agagaggatc tgtactatcg cctaaatgtg ttccccatcc atgtcccccc
     7921 tctcagggac aggaaagaag atattccacc acttgtggca tatttcttga aaaaacattc
     7981 aaaaggaatg gatgtgagga ttgaagaagg agcaatggaa ttattgaaac aaaaaagctg
     8041 gaaggggaat gtcagggaac tcgaaaatac cattgcccgg gcgtgtatcc tttcaaatta
     8101 ttcactaatt aagactgctc acttacctga catgaattct gtaaaggatg tttcagtggg
     8161 atctgttcgg gagatggaaa tgaagctgat tattgatgca ttaaaatcag tggggggaaa
     8221 tagaacgaaa gcagcaaatt tgttaggtat aacggcaaga accctgcgaa acaaattaaa
     8281 agactacagg gatctgggtc tgaatatccc tgacaaggag gtctgatgga ggttacagct
     8341 atccttgaga aagccctaaa tatacgggca cagtatcaca aggttttatc aggcaatatt
     8401 gccaacgttg agaccccaaa ttataaagaa aaggacatcg attttcaggc agaaattcaa
     8461 agaaatattg gcggttcgaa tcatgttgaa atacgggaga gctctgatgg ggatgttatt
     8521 gggagcatag acgggaatac cgtcaacatg gaaaatcaga ttgttaagat gacagagaac
     8581 catatgcttt ttacttctct tgttcaggtc atatcaaaaa aattttcaat gatgaggtat
     8641 atcatcagcg aaggaaggag ataatatggg gggcatcctt gacattttaa agattagcgc
     8701 atcagcgctc aaggccgaac ggactagaat ggagatcaca tcttctaacc ttgccaacat
     8761 tcacacaacg cgaacagagg aaggcggacc ttacaaaaag aaagaggttg tttttatcgc
     8821 ttcagatatc tcggaagggg agggttttgg aaggatgcta tccgacagag tcagcagcgg
     8881 ggtgaaggtt gctgagatca aagagagcac gagagatttt gaaaaggttc atgacccagg
     8941 tcatccggat gcggacaaag aaggatatgt tacctatcca aatgtgaacc ttatggaaga
     9001 aatgacagat atgattgctg caacaagagc gtatgaggcg aacattaatg ttattaacac
     9061 cacaaaacag atgtttttga aggcattaga gatagggaaa taggaggggc catgaaaatt
     9121 gacagtattg caggcattgg caacatggtc gaaaataaag gcgtcaacgc tcacaagggc
     9181 aaaacaacct tcagtgaggt tctcaaagat tccatccaaa aagctggaga attggagaaa
     9241 gaagctgatc aggaggcaat aaagcttgcc aagatggaaa cgcaggatat ccataacacg
     9301 atgattgcca tagaaaaagc ggatctgact ttccagctta tgatgcaggt aagaaataag
     9361 atcatcagcg catatgaaga gattatgaga atgcaggtgt aagccatgga cgccttcata
     9421 aaacaggtta aaaagtatat acaaacaact ccaaaaaaca gactctatct ttacctgttt
     9481 cttctcgtcg caataattgg aggatcagtg cttggcctgt cctttatcca gaaagagaat
     9541 tacaataccc ttttctcagg cctgtccaca gaagatgctt ctgcagtggt tgcaaagctt
     9601 aaagaacaga agataccgta taaacttggc cttggaggca cagccatata tgttccaaat
     9661 gaaaaggtat acgatgtacg gcttttgctc gcctctcaga atgctttgcc tggaggtgga
     9721 ggtgtggggt ttgagctttt cgataagaca aattatggca tgacagagtt tatgcaaaac
     9781 atcaactata aaagggcagt tcagggtgag ttagccagga caattaacca gatgccagag
     9841 gtaaaagcct cgcgggtaca catagctatt ccagaaaaga cgctcttctc tgacagagaa
     9901 aaagaagtta cagcttctgt tttcctaaaa ttgaaaccag gaaagaacct ttcaaaagat
     9961 caggtaagtg gtattgtcca tctcgcttct ggcagcattg aaggtcttaa accggaaaat
    10021 gtttctgtta ttgattcatc aggacgcata ttatacaaga acggtgacac aaactcacca
    10081 atggcactat ccggtcagca atatgaatta caaagaggtg tggagaggag acttgaagaa
    10141 tctatccagt cgatgcttga caaatttatc cctgcaagcc agtcaattgt tcgggcgaat
    10201 gttgagctta acctgagaaa gatagagaaa atggaggagg aataccagcc caacaaaagt
    10261 gttattgctg cggaaaagaa aagcagggaa agaacctcaa accagactgc aaaggttggc
    10321 ggtgtgcccg gtgtggcatc aaatattcca accacaggtg ggacacgtga tgtgcgacca
    10381 gaaacgccaa acagggttaa cgaatcagag agagaggaat cgcagattac ctacgaagta
    10441 agcaaatctg tgagcaaaat aatagagcct tttggtgata ttaagaaagt atctctggcc
    10501 atagtggtcg atggcaaata tgaaaaggtg aaaggtcaga aaggcgaaga aaccaaatac
    10561 gttccccgtt cggccaaaga gttgaacgat attaaaaacc ttgttgcaag ggcagttggt
    10621 tatgacgagg tgcggggaga taagattgag gtgctgaaca tcccttttga agttgaaagc
    10681 tctgcagatg aaaaggggat aattgaacag gcagagaaga aagagctcat attcaatgtg
    10741 agtaagtatg ttttttatct ggttattttt gctgcagtat ttttctttgt tatcagaccc
    10801 cttcttaata tcttacagaa aagaggtcca gtacttccct tgaaaaaggt acgagatgta
    10861 tatgtgaaag gacctggtgc tgatggtgca actgcagcaa ttgaggacaa ggcacagagc
    10921 gctatatcag atgctttgaa ggacaaggcc cttgttggaa cgatcataaa ggagtgggtt
    10981 aaggaaggaa cgtaatggaa gctcaggata ttccgggaat taaaaaagcc gccattcttt
    11041 tacttacaat ggatgaggaa atctcaaagg aggttataaa agatctggaa gaagaagata
    11101 ttgaggctat tggtaaagag attgctcagt tgaaaattat acctgataat gctgttgcca
    11161 ctgtcaggga tgagttcatg aggaggcttt caaagcgcgg caatcgcgtg gttggtggag
    11221 agaataagtt taaggaactc ataaagaaaa gctttagcaa tgaacaggcc gaacagtttc
    11281 tggaaaacat ggagacaaaa aaagggctcc cgggagattt tcttcgcaga tgtgatccga
    11341 aagtcttagc aaatataatg aaaggtgagc atccccagac catcgcgctt gtgttgtcaa
    11401 cacttggaaa taagaaagca ggtgaagcaa ttgttgcgtt gcctgagaag gttcaaagcg
    11461 atgttatagt tcggatggca tttctggaaa aagtagatac caaggttctt gaggaaattg
    11521 aaaatgtgtt gcgggagcaa ctcgagtcaa ttggtgttgt tgaggggagg cagctcggag
    11581 gagtcgagat ggtggcaggc atcttgaacc agatggacag aacccatgaa tctgaacttc
    11641 ttgaaaagct tgaagagatt gagcctgaac tggcagagag gataaaacag ctgatgttta
    11701 ccttcgagga tcttgtgcaa ctagatgata aaagcgttca ggctcttctg aaagagattt
    11761 cctcggatga aatatctgtg gctctcaaag gtgcatcaga tatgatgaag gacaagatat
    11821 tctcaaacat gtctgaaaga gcagcagcca tgctcaaaga agacatcgag gccatgggtc
    11881 ctataagact atcagatgtg gagaaggcgc agacaaagat tgctatgata gcgaaaaagc
    11941 tcgaatcaga aggaaaaata gttctctcga gggggaatga gaaatttgtc taaaggtata
    12001 acggtttaca aaatagatcc ctttgcccag gaggaaagca gcaaacaacc agaatttatg
    12061 agtatttttg atgattctgg aaaaaaagca aagaatgacg ataaaccgaa cagtgaagaa
    12121 caggcgcgaa aaatatttga agatgcattc gtacagggtg aaaaagccgg gtacgaaatg
    12181 gggatgaaaa aaatcgagca gattgcaaaa cgtttgaaca gctatattac agaattagat
    12241 gtttttaaag ataagctcct gaaacaggct gagtcattta gcatggaatt agcgcttatt
    12301 ttcgcagaag cgcttgtgct tcgggaatgc tcggaaaaga agggcctggt tatggatatg
    12361 gcaaaaagag cctttgaaat ctgtgaagac aagcataaca ttgtcatcag ggtaagaccg
    12421 gaagatgctg aatatctatc caaaagcaca acaggtcatc tcaagattgt cccagatgat
    12481 acccttaagg aacctggttt tatcgttgaa acggaatttg gtgatataga tggtagattg
    12541 tcggtccaac tcgaagagct aagaaaagag tttctcaatg tacagcctga atgaggatca
    12601 gctcagcata acaaagaaac gcctctctga tttctatccc tacaaggtat atggaaaagt
    12661 gaatcaggta gtggggcttg tcatcgaggg aaaagggcca atttcatccg tgggtgacgc
    12721 tgcccttata tttcctgttg atgggtctga cccgattgat gcagaagttg tagggttcaa
    12781 agatggtaaa actctgctga tgccgcttgg tgatttacgg ggtgttggga ttggttcaaa
    12841 aatcctgtca aaaaaacaag cagttaatac ggctgttggg gagggccttc ttggcagagt
    12901 catcgatggt ctcggcaatc cgatggatgg tggtgggccc atcgactgca gggaactgat
    12961 tcctatttat cgtgacacta taaaccccat gaacaaaaag cgaatcacag agccccttga
    13021 tctgggcata cgaagcctca atggcctcct gacctgtgga aaagggcaga gaattggaat
    13081 attcgcaggt tcaggtgttg ggaagagcgt actattaggt atgattgctc ggcatacaga
    13141 agccaatgtt aatgttatcg gccttatagg tgagcgtgga cgggaagtga gagagttcat
    13201 cgagcgggat ttgggtgacg ggcttgaaca ctccgttgtt attgtggcaa cgtctgatca
    13261 acctccgttg atcagggtaa ggggggcatt ccttactatt gcaatagcag aatatttcag
    13321 ggatcagggc aatgatgttc tccttctcat ggattccctg acaaggtttg gtatggctca
    13381 gcgagaggtg ggacttgcga tcggtgaacc acccacttca aagggatata cgccgagtgt
    13441 gttttccctt ctcacaaaag ttctagaaag agccggaaat ggtgaaggag aagggacgat
    13501 gaccggcatt tatactgtat tggtagaagg ggatgatctc gatgatccga ttgcagatgc
    13561 tgcgcgttca atccttgatg gacacgtggt cctttcccgt aagcttgccg acaccaacca
    13621 ttatccccct gtcgatgtgt taagaagcat aagcagaaca atgaaagaca ttgtgtctaa
    13681 agaacagatc gaatatgctt caaaaatcat tgaagtctta tctgagtttg aacgtgctga
    13741 agatttaatc aacatcggtg cttatacacc ggggagcaac aagaagattg actatgccat
    13801 atctatgatt gacaaaatga aactatttct aacccagggt atcgatgaaa agacaacaat
    13861 tgaagaaacc tttaatagta tgaaaatatt attttatgta taacaattga tccagtcttt
    13921 taacgaatgt ccaggaggat gcatgatgga ttctaagaac aaaaaaaaga ttgtgaaaca
    13981 agatatggat gatcccatca agcctgattt gtcaaaaagt ggattaccgg tagcctatca
    14041 acaacttctt agtctcattt tcgaatcccc agaccctctt atctataaag gatttttcca
    14101 tgacaaaatt tcgtcttagc aggattatcg atgttaagga aaaacttatt gaagataagg
    14161 aaagggaatt ggaagaagca ataaacacca tagacgacat taccttggct ctcgatgcaa
    14221 cagagaagga tgttgagaaa acttataatg agttggcaat cccctcactg agtggtggtg
    14281 atttttccgt gttaaaggat tacctgtcct atcttaacga cagaaaacag cgccttattg
    14341 atgaaaaaga tcttactcaa cagagaatag agcaattacg gattaatctt attaatctca
    14401 tgaaggaatt gaaaatgtta gaaatccttc gatcaaaggc ggctaaagtc gtcaagagaa
    14461 ctgaaaacag aagaatacag aaaaaccttg attccatggc ccttcgcata ggcgaaagaa
    14521 gaatataatc cctggtgaat attctgccat caaacagacc ggtcttcgtc caagaaagag
    14581 ccggttactc agaacgctgg ttcctcgtat caccttaatt ttttattcat ggtttttaca
    14641 agctggtagg aaaaaatttc ctcttaagga actaatttcc taaagcccaa acgagataag
    14701 gtaatgcaaa aactcgattg ttgttgaatt tattcaataa attaaaatgg cacgtgtttt
    14761 gatttaatac attgccatgg acagcctact tataaactta attggtattg gagctcacgg
    14821 cggacccgcg agtaacgcct caccaaaaga agactcagga actgaattta atcaaaaaag
    14881 ctttttcgaa gtcttgaagg ctgccatttc agaaggcagc agcaaaacat ccttcctgaa
    14941 caagcttgac gaaattgggg gatcaggtga atcaaacctg gcgaatgtca tgagctcaat
    15001 ttccaacatg cttttatcta tgataccacc agccatggta ccatcagtgc cagccagtga
    15061 aactcttgac gctggaggac aagaaacctc tgaaatggtc gaagacgcca tggatacggg
    15121 ccaagaagga ttaagcagtg cgggaaagca gataaaagta aaggaattgt gcagcttgtt
    15181 ggaacatgca aatctttctg cacttcagga aggtttgtcg gatgaaaaac cagatactat
    15241 tgaccatgaa accattaata ggatttcagc aggtattgtg aaggaaggtg aaacggagac
    15301 agaagaagaa caattcatgg cttttcagga agaaatgata aagcttctat cttttctgaa
    15361 aggcaaacct gagttatcgg gtgaaaataa taagagttct gcacagggtg aacttggaaa
    15421 agcgatcgtt caaagcactc ctattatcaa tcttcaacag accgccctta aggtagattc
    15481 caatacagac agcacagtaa cacaggcaca gaatatacca atcatttctg agctgcccat
    15541 agaagagatg atggagaaaa atatttcctt ccttcaagct aacgtgcaaa acagaggtaa
    15601 gtttgaccaa gatgatggca aaaagctcga taataacaga aacaatgaag gtatggatgg
    15661 tatatttgag cctgaccagg catttgccaa gaccacggtc aaacagtcat ttgtcaccga
    15721 atatggactt aacgaagaaa aagggcgaga aacaaagggc gaaatccata cccttttcct
    15781 gaatgccgcc aagaaataca aggagcatgt atcagaaaca aatgagtcca acgcagtttc
    15841 agacagtgat atggctaaca tgtctccaca gaaaccaatc attgaagtgt cacaaagcga
    15901 ccagttcaga gctaatattc tcaatataaa agataataat ataacctttg aaaaaggttc
    15961 atttacatca ttcatgaccg atagaattga aaaaatcgtc gaacagtatt cgaacagggg
    16021 gtctcagatg gacatggttg tgcgtctgaa actggatgat aaagaaactt tacttattgg
    16081 ccttaaacat gagggacaga agataattgt cgacgttaag gcatccaatg atagtcttgt
    16141 gaaccttatt cagatacaca aagatgatat tgctcgacac cttgaaggca aaaacatatt
    16201 tacaaatatt tttgtacagc ccgatggaga gaggaacact tcaaaacagc atgaccgcga
    16261 gaacaaaaaa ggagaaagga gacaagaaac aagagcttct tttatcaata tacttgaagc
    16321 tactgtttag aaagaggtga ccatatggga atacagagtg tcggtagcag ctataccaca
    16381 acaacaacgc agtcagcatc aaattctggc aataagcttg ggaaagatgc ttttttgcag
    16441 attcttgttt ctcaactgaa gtatcaggat cctatgagtc ccatgaaggc tgacgaaatg
    16501 ctctcacagc tttcccagct cactcaggta gaacagttga caaacctgac agcttcaatg
    16561 aatgccatga ggaaggcagg agacgttagc cagtggattt caacgattgg aaaaaaagta
    16621 aatgtcagta ccaacgcgct ttcaaagggt gacgagctga cactcatgcc gcagaatgat
    16681 tacgaccgca ttgtggtaac cctcaaaaac ctcaaggatg gatcatcgag ggatataacg
    16741 atcaataagg gtgacttgct cacatataca catgatggag atgacatggt tcaggtttca
    16801 gtgaaagcaa tgaaggataa caagcaggtg tcttgccagg cagtggtatt caaaacggta
    16861 cagggtgtaa tagtcgcaga ctcaggccct gtcttagttt ttggtaatga tgaaacatat
    16921 gatgcatcac aggtacaggt tattaaaaaa taggagggag acatgttaag ttcatttttt
    16981 tcaggaatca gtggcctcat ggcaaacagc tatggaatca atgttgtggg taataatata
    17041 gcaaacgtga acaccatagg ctacaaaggg gcaagggcta catttcagga tgttctttac
    17101 cagtcggtct ttggcactgc ggggacgagt cagataggtc ggggtacagc tctcagctct
    17161 gtcgatacaa tctttgctca gggatctttt gagagtacga gtgagcccac ggatctggca
    17221 ataggcggca aaggattctt tgttgtgagg cagctcaatg gtgatgatcc agcaactgat
    17281 cagacaaatt tactctatta tacgagggct gggcagttca gatttgacga gcagggatac
    17341 cttgttaatc cagcagggtt tctggtccaa ggatggccaa tagatagagt gacccatgaa
    17401 cgtgtcggcg tctatggcga cattcttgtt tcaccccagc cgagcgagcc cagagagaca
    17461 tcgttcatag ctatggcttt gaatctgcaa gcaaatgcaa gtatacttcc tgcctttgat
    17521 ccatctgatc tagcaggaac atctaactat tcctcttctg tcactgttta cgactccatt
    17581 ggccaaagcc atgttgtgag cgtgtatttt cggaaggttt ctgatggaac atgggaatgg
    17641 catgcacggg atgcagacag cttatcaaca gaaccagatg caggctcagg aaccctgaat
    17701 tttactacga caggtgtgct tgacgctggt agcggagcac ctgcaaatat cacactcact
    17761 tttcctaccg ggacacagac cgtgcagctt ggatttgatg gttcccttgg gggcaaccca
    17821 acaacacagt atcccatggc atctgcaacg aattttcaaa cccaggatgg atacccaccc
    17881 ggggtattat tgaacgttac agtgagtcct gagggtgtaa tttcagggca ttactcgaac
    17941 gggcaaatat tggatcttta tcagttaaca ttagctaact tcaataaccc gtgggggttg
    18001 acaagggagg gtggcaacct tttctctgaa accttagaat cgggtgtacc ctacacaaac
    18061 ccgcccggtg ttaacggtct tggcaagatt aaccctaact cccttgaaca gtctaatgtt
    18121 gacctggcaa ccgagtttgt taagatgatt gtttctcaaa gaggttttca ggctaattcc
    18181 cgggttatca caaccactga tgagattctt gctgagctga tgaatataaa acgataaatg
    18241 atggaagtga gaacaggggc tgatttccca gcccctgttc ttttttttgt ctccaaagct
    18301 tagcaatcag caatactcca ccatattcga tagtaacctg ttttaattcg tgtcaaatgc
    18361 atgacaaaac ttgacccaac atgtcatggc cagctttttg cacaaacctg aatccttttt
    18421 tatcacgtta aatttacaat tataacagta agttacagtc attatccttt ctggcatata
    18481 tattgctttt ctacggtcac ggaggatttg tatggctgac gacgaacaga tggttatgga
    18541 aacagaggac agcgaaggca cgccccaaaa gaagggtggc aagctaaagt tgatcatgat
    18601 cattgtcctt attgtccttg tcggcggtgg cgcaggggta tattttgtct ttgggaacca
    18661 gttgaaggca agatttttcg gaggcattga gcaaccggtt aaggatgaga aaaagaggga
    18721 atctataggt ccgatcctat ccctcgaacc tttcatcttt aacataggag gggcaagcgg
    18781 aaaatttgcc aaaattaccc ttggggttga ggtaaaggac gccaaggtaa tggaagaagc
    18841 aaagaaaatg gtgccggcaa taagagatag aactttgtct gttctaggtt caaaagggcc
    18901 tgaggtgtta atggatatca acaacagaaa ccatcttaaa aaggaactgc taaacgctat
    18961 gaaaaccctt tttaaagacg atgctgagct gaagtcatta tatatcactg atattatcgt
    19021 ccagtgagga gttgggatgg aacagatatt atctcaagat gaaattgatg cccttcttaa
    19081 aggaatctcg gaaggcgaga ttgagactca gaaagaggat tatgaactcg ccgaggttgc
    19141 tgaaaatact gttcaagaaa agatagaagt cagaaccttt gacttcctca agtatgcaag
    19201 gagtaaaaag gagcggcttc cagccctcga atttatttat gataggtttt cgaaatcttt
    19261 cagatctgct ctttcacttt ttgtggaaaa ggaagttgag gttaatcttg agccaatgca
    19321 atacgtctct tacggtgatt ttatagagac ccttcctttg ccaacaaaca tgaatgttgt
    19381 agtgactgaa aaacttaaag gttttttcat agtcattttc gatacaaaac tcattttctc
    19441 ggttttggaa acaatttttg gtagtgcaaa tacatcgaaa aaacaaaaga tagagggaag
    19501 agaattcacg aagattgaat tcaatgttat caagaaattg attaacattg tatgcgccga
    19561 gatggaaaaa gcctgggcgc cagtgcacga gatatcatgc aaatactctc gttcagagat
    19621 taatcccaat tacataacaa tgattgtgag tgaagaaacg gtgaatgttt gtgaacttac
    19681 cattgaggta gaggggatca aaggatggat gaagatatgc ataccttacg gaatcctcga
    19741 gacaataaag ggacacctta tctccacgcc atcccatgag gattcagaaa tgagggagaa
    19801 gtggataatg aaaataaaag aaagaatcaa tgacgtaccc cttgaactga aggcaatcct
    19861 gggcaagaag aagatggcgt tacaggaatt tcttaagctg tcagaagaca atgtcattat
    19921 catagacaag tatgtgaatg acccggttga gattgaaatc tgtaacagaa gccgaatcag
    19981 gggcaaatta ggaatatata aaggcaataa agcagtcagg gtagaggaga taatacgtta
    20041 atggttatgg agaaccatac tcgccatgaa ctgtgcatta aaagtgcaga gttcatgggt
    20101 tgaaagaggg aggtaagatg gaagaatttg taactgaaaa caaaaccgta tcatctgcaa
    20161 aaaagatcga actcaatatc gacaacttgc ttgacatatc ggttgaaatt tctgttgaga
    20221 ttggaagaac caaaatgtcg attggtgagc ttcttgccat gtcgaaaggt tcaattattg
    20281 aactgaacaa actggccggt gaatctgtag aagtctatgt caatgaaaag ctgcttggaa
    20341 aaggcgaaat agtagtagca aacgagcggt taggggtaag ggtcaccgag attgtgacac
    20401 ccaaagagat ggttcaaaag ctgggataat ggatgcttca ttcaacataa tgcgcgttat
    20461 tcttgtcctt attgggattg ttgcgggaat ggtttttctg tacagattgg caagcaagta
    20521 taattttaat tttaagccaa aaagtacgac agaatacggt ctcaagaaaa tggacacaat
    20581 ccatctcggg tataaaaagt ttatttcagt ggtagaagta aaagaccatg ttcttgttct
    20641 tggtgtagga gaaaaagaga tgtctcttct tacaaaatgg aaaaaagagg ataaggtaac
    20701 gtgaaattgt ttctaattgt catgattgta tgcaccttaa ttattctccc tgcttttgct
    20761 tcggcaaaag cggttgagag taaaggcaga actgagtcga agatcgatat tatgaattct
    20821 ttcaccgggt ctgaagggag taagaatctg ataagtgttg tcctcctttt gactgtcctg
    20881 gcttttgcgc cagccatcct gcttctcatg agttcattca caagaatagt aattgttctc
    20941 tcatttttga ggcaggcaat cggcattgca cagataccac caaaccagat aataattggt
    21001 ctttcacttt ttttgacctt ttttatcatg gcccctacgt atgaaaaagt tcattctaca
    21061 gcgctggcac catacatgag caacaagata ggctatgaag agtttatcac caagtcatct
    21121 caagagatga gtaccttcat gctgaaattc acaaaagaga aggacctcgc cttgtttgtt
    21181 ggtcttgcag gaatcacgaa accaaaaaat gcgagcgaga tacctcttcg cgttgtagtt
    21241 ccggcatttg ccattagcga gttaaaaagg gcatttcaga ttggtttcct tctctacatc
    21301 ccctttctcg taattgatat ggttgtggca agcgttcttc tctctgctgg tatgatgatg
    21361 ctgcctccca tatttatatc actgccattc aagctgatgt tgttcgttct cgttgatgga
    21421 tggaaccttc tcattggttc cataataaaa ggattctatt agagaggtgc ttcttgagcc
    21481 aggatcttat tgttcaaata ttcagagagt ttttaaaaac cgctatcttc ttaatgggtc
    21541 cgattttaat tgccacgatt atcgtaggtc ttctcgttag catttttcaa gcagcaacac
    21601 agatacatga gatgaccctt gtatttgttc ctaaaataat tgttatcggt ttgtgcttga
    21661 tgattctctt cccgtggatg cttaacctta tggtctctta tacagtgaac ttgttttcaa
    21721 atataccgac atatgtgaaa taggatgttc atggatttta caaacgacat tcaagcattt
    21781 ttgctaattt tttccagggt ggtgtctgtc ctctggcttt tacctgtctt ttcatcgagg
    21841 gctgtatccg ttcctttcaa ggcaggggta tcccttctaa ttgcttatct cctttttgat
    21901 tctattggcg ttgaccggtc gttcagcaat gatccatacc tgctttctct ttttgttatg
    21961 aaagaagtat tgataggcct tgccattgga ttttcggtac ggatcctctt catagctgtc
    22021 aacattgcag gagagattat ttctatccag gctggctttt catttgcccg atttatggat
    22081 cctttatcta tgacacagac aacggttata gaacatctca agagtcttct cgccatgatg
    22141 cttttccttg ccatggatgc ccatcacatt cttgtaaaag ctattgtggt cagtttcaga
    22201 gatgtcccga tagggagtgt cacaattcaa caatcatttt ttcagtactt tatttcggct
    22261 accggtcgca tatttatgac tggttttaaa attggcgccc ccataattgt aacccttatg
    22321 tttgttgaag ttgctctggg aatgctttcg aggatgatcc cacaaattaa tatttttatc
    22381 gaaggtgtgc ctttgaaaat tcttgttacg gtaactatgt tttccctctc ccttggtgta
    22441 attgttcctg ttattgtcaa tatgttcaag ggtatggacg gggagatttt aagattattc
    22501 aggttgatgg tatagaagat ggcagaaacg tttcaggaaa agactgaaca gccaacggaa
    22561 aagaggctcg aagaagctaa aaagaaaggc caaatcgcgc agtcaagaga actgccatta
    22621 tgtctcatgt ttctctttac cgctattttt ctatatttta tggcctctca cggttttcac
    22681 cagattttca aagtatatgt aaactatata agcaatatca acctcgacgt caactcggcg
    22741 aatattttca gcattctttc ttttggtgct tatcgttggg cctggattat gatgccattc
    22801 tttgcacttc ttatagttct gtctttatca ggcaccatac tgcaaaccgg ttttatgtgg
    22861 agtgctgagg cgatgcagtt caagttggaa aaactcaatc ctttaaatgg gattaaaaga
    22921 ctcttttcca aaagatctgc tattgaagtt ttgaaggcaa ttgtgaaaat tacagtcctt
    22981 gcttacatag catactcgat cattatgcat gaattgcctt caatcctgtc tcttcctgga
    23041 aaagacccag gcatgatcat atcctatctc ggttcaacgt gtttttatct ggctattaaa
    23101 gttggcataa tcttcctttt tattggcgct ctggatttcc tatttcaaaa atggcagcac
    23161 aaaaaagacc tgatgatgac tcaccaggag gtgaaagaag aattcaagga acgcgagggt
    23221 aatcctcttg ttaaatcaag aatcagaagc cttcaacgcg aaatggcccg gaggagaatg
    23281 attgaagatg taaaaactgc tgatgttatt ataacgaacc cgacccattt tgccattggc
    23341 cttaaatatc agtttggaat tatgcccgca ccaaaaattg tggcaaaagg tgcaggcttt
    23401 gttgcagaaa aaatcaaaga ggttgcccgc ctacacgatg ttcctatgat ggaaaataaa
    23461 ccgcttgcgc aggctctatt ttatgctgtt aaagtgggtg attacattcc agaacaattt
    23521 tatatcatcg ttgccgaact tcttgccgaa gtctatcgac agcgaaatag gtcaaggata
    23581 taatgggcag atttgggtca ggcataaaag agaaaagcga tatattcgta gcagtcagcg
    23641 ttgtctttgt tatcctcatc atgattgtcc cactgaacgc tttctttatg gatatttttt
    23701 tgaccctcag catctctctt tccatactga tactttttat tggcatgtac attaacaaac
    23761 cccttgattt ttctgttttc ccttctgttc tcctcattgt gacgcttttc aggctttctt
    23821 tgaacattgc tacaacccgg cttgtgcttg ttcatggtga cgagggggcg catgcggctg
    23881 ggtacattat aaaaggtttt ggaactttta tcgttggcgg caattatgtt gttggagcag
    23941 ttgtcttctt tattctaaca atcatcaatt tcgttgtcat tacaaaaggt gctggccgtg
    24001 ttgctgaagt ggcggcaagg tttactctgg acgcaatgcc aggtaaacag atgagcattg
    24061 acgctgattt aaatgctggt cttattgatg atgctggcgc acggaaacga cgggaatctg
    24121 ttgaaatgga ggctgatttc tatggcgcca tggatggtgc gagcaaattc gttcgcggtg
    24181 acgccattgc aggcattatt atcatctttg taaatattat tggcggactt gttattggca
    24241 tcgttcagaa aggaatgtca tttaatgatg cgttaacggt ttatacaatc ctcactatcg
    24301 gtgatggtct tgtaagtcaa ataccagccc ttcttacctc tacagccgca ggtatcattg
    24361 tcacacgggc tgcaagtgag acaaatctcg gcagtgcaat gataactcag ctttttactc
    24421 aaccaagggc catgcttctc gcatcaatag taatcctctg catcggcttt gtgcctggaa
    24481 tacctctcgt cccattcctt gttttatctt cctgtacatt tggaactagt taccttgtgg
    24541 gaaaaactaa gaaagaggag atcgtgagtg ttccggaggt gactggtgag gagagagata
    24601 aaggggaatt catccagccg atggaaattc tggaaataga aataggatat ggacttattc
    24661 ctattgttga tgctgagcag agtggagaac tccttgacaa gatcagggct attagaaaac
    24721 agatggctat cgagcttgga atcgttgtgc cgccaatgaa gttgagggat aacctccagt
    24781 tgaaggctgg tgaataccta attctcctca aagggataga gatgggtcgt ggagagctca
    24841 tggcaggata tgtcctcacc atgggacatg aagataaaaa gaaaaccata gatggaattc
    24901 caaccaagga accagtgttc aatctggatg cttactggat aaaagaaaag gatagagatc
    24961 agtatgttgc tgctgggatg acagtcgtag atcatcccac catcattgca actcatctca
    25021 cagaaatcat aagaaataac gcccatgaac ttatgaccag gcaggagact caaaaactga
    25081 tagataccgt atctgccact cattcaaagg tcatcgagga gattgcacag aatcagatta
    25141 atgcaggaat tattcagaag gtgctgcaaa gccttttgag ggaacaagtt tccataagag
    25201 atcttataac gatacttgag gcggttgcag atgcatcatc cacatcaagg gaacctgagc
    25261 ttatcaccga gtttgtaaga cagcgcatgg caagaagtat tctaaaacca tacttgattg
    25321 atggaaccct caatctcctc attttggaga aaaaccttga ggagaagttg attaacagtc
    25381 ttcagccttc ggaccaagga acctatctcg cccttgatct cgcatttagc cagcagttga
    25441 tcgaaaaggt ggggaatgag gcaaaaaagg caatgctgca gaatattcaa cctgtaattc
    25501 ttgtacaccc agttcttcga agtaagttac gccgctttct ggagcggtat atacaaggca
    25561 ttaccgttat ttcacataat gagattccgc ctcagatcaa aattcaatcg ttgggggtga
    25621 ttaaagtcaa tgaaagttaa aacctatgtt tttggagatg taaagaccgg tatggaagcg
    25681 atcaaagcgc agcatggttc tgacacaatc atcgttgaca ttaagaacaa tggaaacaat
    25741 acatccccga agagttgcga gatttccatt gctgttgatg aagacttacg gccagaccag
    25801 tgtgatctca ttgatgtgag aaagaaaacc gaggcaatat ggaactatgc tctgatgctg
    25861 atgaacgaac gactttccgg cattgagaat gacataatca gagagaggac acgagaatat
    25921 ccactgccgc tgaggattct ctttgataag ctgattaaaa atggttttga gcgtaaggtc
    25981 gccatgtcct tgatctctga ggtatacggg gagataggag atagagcgca ggatagtatg
    26041 aaagcgaata cttccatgag aagggctata gcacagcgga tttcaataag taacattact
    26101 gaagagagcg gccctgttct gatgcttggg ccaacaggat cagggaaaac ccagacgact
    26161 aaaaaacttg cgaaaatttt tgctgccagt gataaagcca tctctattat cgcgtatgat
    26221 ccattcacga aaggaagcta cgatgatctt atgtcatttt cggagagcca cggtattccg
    26281 ttttcgttta caacgaatgg ggacgatatc agctttattg tcgagcggga taaacgtcaa
    26341 aaaattatcg atgtgggcgg gcatcccaat attcagagaa gggttctaga aaaaatacga
    26401 gatataaata aaataatcgt tcttcctgca ggtgcacgtg acgagaagat gcaggcatac
    26461 tgcaatgaat ttggtgatga tacaaactcc acaattggat tcactaagct tgatgaagaa
    26521 gagaatcttg gccatatcgg gcacaacctg attaaactgg gatatcctct aagcctcctc
    26581 acaattggaa taggtataga tgacattgta ataccagata acgaatcatt atttaaaata
    26641 cttttagagg gtaacatatg gaaaaaagaa gaaagaagac cattaccata acaagcggaa
    26701 agggaggggt gggcaaatca tcaatcgttt ccaatatggc atatgtattg ggtggaatga
    26761 aagcatcaac atatattctt gatgccgacc tttctctggg aaatatagac attatgttcg
    26821 gaatggctcc acagttcaac attaaggatc ttatcaatgg tagaaaaaga attggcgaga
    26881 ttgttgttga aggtccatgt ggtatcagga ttattccagc cacttcaggg gtaacagaat
    26941 tttcccatct ttcccttgat gagaggaata tacttctcac atcttttcga gaattgccgg
    27001 agtatgattt tcttctcata gacacttcgg ctggtatctc atccaacgtt gtctatttca
    27061 acgcaatcag tgaagatatc tttatcgttg taactccaga tccagcaagc ctaacagact
    27121 cttatgctgt catcaaagtg ttgcacaaga acacaggaag aaagaacttc aatgtgattg
    27181 tcaacatggc caaggatgaa aatgaagctt tggatgtgtt taagaagata acccgtgtta
    27241 ctgatcgttt ccttgacgtg tatcttgatt tctacggcta tatccctgtt gataagaata
    27301 ttaacgtggc tacgagaaag caaaaacttt gggtagagca gtttcctaat actgttgcga
    27361 caaaagcatt acaaaaaatt tgtgacagaa tggtggcgtg agatatgtgg aagagagcat
    27421 atgcaaaagc acaagaagac gaccgggaaa aaacaataga ggaatttatc cctattatca
    27481 aaaacctcgc ctataaaatc tcaagaggat tcgaggataa ctgcatcatt gacgatctta
    27541 tttctgctgg tgtcatggga ttactcgagg ctatggaaaa gtttgatgtg tccagaggaa
    27601 taaagctgaa tacctttgcc tatttacgga tacgcggtgc aatgatcgat gaattgaggt
    27661 ctcgggattg gtttcctcgg agcgcacgat caaaagcaaa aaaaattgga gaagtaataa
    27721 gaaaacttga atgtaaactt ggaaggtatc cggatgagga agaagtggca gaagcgttga
    27781 acatggatcc ccaagactac cttaccatgc tcaaagatta cggaaatcta tcaatcctga
    27841 gcatagaggg gatatcagag atttcaggag aagatagaga cagaatcata cagtatgtga
    27901 tcgatgagag cgatgatcct gaacaatatg cccagtttca tgagattgaa agaattttag
    27961 cgcaggaact tgaacggctt ccggaacgac aaaagcttgt tttaactctt tattatctcg
    28021 aagaccttaa tatgaaagaa atcgcaggaa ccatgagtat aactgaggcg agggtatgcc
    28081 agattcattc tcaggcgatt atgaacctca gaacatccat gaggaaacac ctgaagaact
    28141 aaagttggtt ttgcgattac acgataatat taaaggtatt cgcatggcgg gtttggacac
    28201 cactctcaag aaaatcagga aaattaatat tttgcccacc tttcctgata ttgttgacgg
    28261 aataataaat cttattgaag atccaatgag ttctgcttct gatctcgcaa aaaatatgga
    28321 cccttcaatg gtgggggaag ttatgagggt agccaataca gcttattttg gcatgagaag
    28381 tttcaggaaa ataacaacaa ttgagcatgc catcgcaata attggctatg aacatctttc
    28441 acacattctc ctgcaaatgc cttttttgaa gatggtccag gtaaatgatg aagcttttga
    28501 tcgcaatggt tatattcgac attccattgc atgtggtgtt atggcaaaga taatgagcac
    28561 cacccttcat attgcaaatc cccatgaggt gtacataagc ggaatcattc atgacatagg
    28621 aataataata atctaccagc atttccgcca ctcctggaac gctataaatt cattagtttg
    28681 caccaagaat gttccaagac tgactgctga gagagaggtt ctttcagttg atcatggttt
    28741 cataggtgcg gtattgctcg aaaattggaa cattcctcaa agtattacag aagcagtgaa
    28801 attccaccat tcccctttga aaacacaaat ttttaaagac aatgcagtta ttactcacct
    28861 ggctaatgag ttctcaaaaa ggatagattt taacaaagat tttagtaatt tcaattactt
    28921 tttgagcaga cacagagatt atatcgatta tatggttgac ttgggagggc attttagtcc
    28981 cagcaatgaa gtgatgtttt ttgagaagat atacagctca ctggatggtg tgcgcagata
    29041 ccttcatggg actatggagg gtgagaagga agtagatgat acgtgtaatt gttgttgatg
    29101 attcatcttt catgagaagc attctcacaa gaatgcttga gaaggataag gatattaaag
    29161 ttgtggagac tgccgcaaat ggtgaagaag ctattgataa aatcgagagg gtgaagccgg
    29221 atgtggtcac tctggatatt gagatgcctg taatgaacgg catcgaagca ctaacgaaga
    29281 tcatggccac taaccctgta ccagtgatcg ttttgagtgc tttaacaaga gaaggtgctg
    29341 aagtgactat ggaggctcta aatcttggtg cctgtgattt catgacaaaa gatatttcag
    29401 atggtccgtt gggtatggcc tcgaaagaag cagagctaat cggtaaagtg aaagatgtag
    29461 ccaaaagaaa gttcagcttt ttagtgagac ggctggaact tttaaagcga ccagtgttga
    29521 tcaacaggga caagagaagc aagcacgcag tgctctcaat aggagcatcg acaggtggcc
    29581 ccccagcact gcagcatata ctgacgagtt ttccgcaaaa ttttccagtc cccattgtta
    29641 tagcccaaca catgccaaag ttatttaccc aatcttttgc tcgtaggctg aatggtcttt
    29701 cccagataga ggttaaggag gcagaagaca aggaaccttt aaggcctggc ttagccttga
    29761 ttgcacccgg agatgtacat atgggtttga aaaaaaacgg taaagatata actgttgatt
    29821 ttgtcaagga tgcccaatat gtttacagac cttcagttga ccacttgatg ttttccaccg
    29881 cgcaaatata cgattcaagc gcaattggtg ttatcctcac cggaatgggc aatgatggtt
    29941 tggttggttt aaaggaatta aaacggagaa atggttttgt cattgctcag gatgaagaaa
    30001 cctgtgttgt ctttggtatg ccaaaagctg taatcaacgc aaatattgca gatgttgtat
    30061 tgcccataga gagaatctct gaggagatca taagagtgtt atgagactga agttgaaaaa
    30121 atatgtagca gatcacaata agagggacag acgattagaa aaagctttat atgcaatccg
    30181 gcagggagat aattatgaaa aggaaaaagc tatagagttt ttgattcgtc ggccaacacg
    30241 aaagacagtt gaactaatac tcccgctgct tcaagaaaag gatacatcta ccagaatggc
    30301 agtggttcag gtcgttcaga agattggtca tatgaacgtc caagctattg tcaaccttct
    30361 tgacatggag aatgaagatt taagggttta tgcatgtgaa ataatggcaa gcatgaaaac
    30421 tccacaaacc gtaccatctc ttattaaagc cctcagcgat gagagtgaga atgttaagaa
    30481 cgcagctgtc attgcccttg gggagtttaa cgatgaaagg gctgtaaaag ccctcctaaa
    30541 catgcttcga catgaacaat ggatagtctt ttccgcgata tgcagccttg gaaaaattaa
    30601 gtctccggca gcggcccttc cactgcttca gatatttcaa aacggcgagg aagagctatc
    30661 ccttgctgca tgcgaagcac ttatggattt tgaaaatgac gatattcttg acaagatgtt
    30721 tcatattctc aaagggtgga gtaaaaagaa acgtaaacga tatatagaaa tgattattca
    30781 acaggggaat gaaaaccttt ttcttcgtct taagcggagt atcgggcagg atctctttga
    30841 acatctcttg ccttatgtcg tgtttgacag ggcagaagta gttccaatcc taaaattgat
    30901 gattcacttc aaaagcaagc aaacctgcga tattctcctc gaaaccctga caaaaataga
    30961 tccagatgaa ccggaatatg gtgaaatttt agggttcttt gcctctctat cccatatatg
    31021 gaaggacaac attaacgaat atgtggataa aggagatgaa tattccttag ctattataaa
    31081 agcgtgtgtg caaaacaaga tcactgtccc cgaagatgta ttgatttcat tatttctaac
    31141 gtcatcagca cccgtaaaaa gggagatagc cagaggtgct ggtataattg ttcaaggtag
    31201 aggctatgat tttctgaagg aggccataaa agatccagac ggccacgtga aaagctatgc
    31261 tgtcaatgta attggggaga tgcagctcaa agaattggaa aatgagataa ttgatctgtc
    31321 aaaatacgga ttcctggatg tgaggatcaa tgcaatcaac gcattaatta aacttaatct
    31381 cattgaagca aaaaaattga tcgatcagtt tgtagagagt ggttcactgg aagacaaaaa
    31441 agtgtacctg tctgtggcaa aaaacatttc gggagatgat aattttccac atattcaaaa
    31501 actgtttttg actaaagatg aagaaatcag aaaagctgta gtaaccataa ttggcaactt
    31561 tatcgatgac gagaggtatg cagcaatttt tcaaaaactt ttgcgtagtg acaatgttcc
    31621 tcatgaagcc ttaaagatca tcagggagaa gaggcttgcc cagtttaaac cagtacttta
    31681 caaaattttt tctaatatgg gcaaaagtct atggacgagg tattatgctt tactcgccct
    31741 cggagcattt gaagatcccg cgctttttga attatttgtg aaaggtctgg atgacgaaag
    31801 caacctaata aagattggaa gtcttaaagc cttgagcgat ctcaaagatc aaaaggctgt
    31861 caattatgtg aaaccattaa caaaacacac ggatgatgac gtcaggtcga cagctgaatt
    31921 tgttatgaat aatcttagct attcttagga aggggaaggc aaggttttga ccaaagaaga
    31981 gtttgtgctt ttgagagatt atgtttatga aaaatctggg attcattttg cggaaaataa
    32041 ggcgtatctt cttgaaagcc gtctctccaa cagattgaca gaacttggtt tcagtaactt
    32101 cgaagactat tattatttcc tgaaatatgg gggtgataaa gtaagaagtg agattataaa
    32161 cctattcaat gcagttacca caaacgagac aagttttttc agaaatcccc cacaactgga
    32221 tgcttttaaa gttatagtcc ataagtgtta tttgcagaat ggcataccct caatgccaat
    32281 ccggatatgg tgtgctgcct gttcaacagg agaggagcca tacacacttg ttatgatact
    32341 gcttgaaatg atggagaatc tcaaaaagaa catccccttt agtgtttttg gaaccgatat
    32401 tagcacaaaa gcgctggagt cagcaaaaaa agcacattac aacatgtata gtattcgcaa
    32461 tatgggtgaa tcgataagac aaaaatactt tgttgaaaaa gatggaagtt ttatactgaa
    32521 ggaaaatgta aaaaaatacg tcaaaattga ttttatgaac cttatggata cgaatgcgta
    32581 caagatgtat aagcaaatgg acttcatttt ctgcagaaat gtgctgatct attttgatga
    32641 gaaaatgaag aaaaaggtta tcgattcact ttatgaatcg ttgaaactcc ggggatactt
    32701 aacaataggt cacgctgaat ctcttcacaa catctcacga gcattcaaac cccttatatt
    32761 tccgggaacg attgcttatc agaagggata gtgtatgaaa aagattctta tcgtcgacga
    32821 ttcatctaca atccgcaaac tgataagata tattcttaaa aaacaggaat acaggataac
    32881 cgaggctaag gatgggatgg atgctatgga gaaactagcg agtctcgaag ctgatcttat
    32941 aattgttgac ctaaatatgc cgaacatgga tggcattcaa tttgtcaaaa atcttcgtag
    33001 caattactat tacatggata caccggtgat tatgcttaca acaacgaaag acgcagaact
    33061 taaacgtgat gctttgttag caggtgtcaa tcttttcttg aataaacctg ttcagccaaa
    33121 ttttcttatt tacaaagtag agggcctatt aaagggagag tagcgatgga cgaaaagatg
    33181 tctcaaaatg aagaaatgaa agagatcata gaagaattta tcgttgagtc caacgaactc
    33241 atagaaaagg ctgtagagga tgttgtaaga atcgaaagaa atcccgatga tgaattaata
    33301 aatggaatct ttagggctgt ccatacaata aaagggactt cgagtttcct cggatttaat
    33361 atgctgtcaa atctttccca taaagcagaa gatgttctgg gaagaataag aaagaaagac
    33421 atggagccag atgccgatat tgccgatacc ttgctcgagg ccctggattt tatgaaactt
    33481 atgatagacg acatccgtgt aaagggtact gaaagcaggg acgttagcca tacgctcagg
    33541 aaattggaat ttctcaacaa ggctgacaca cgcaaaaaaa ttggtgagat tcttatagat
    33601 gagggggtca tttccaacag agagagagaa gaactccttg agaaacaaaa acaagatggg
    33661 aaaaaactgg gtgagatagc cattgaagag aagcttatta cggaaaagca gttgagcaat
    33721 gttcttgcaa aacagcaggc ccataaggaa gagcagacaa taaggatcga tgttagaaaa
    33781 cttgatgaac tgatgaatct tgttggtgag cttgttcttg gcaagaaccg attgatactc
    33841 ctggacagtc tcgcacgaaa gggttccaat aataacgcga tgctagatac gctcagcgac
    33901 gtgactaact atgtggaggc agtgaccaac gatctacagc ttgcagtaat gcgggctcga
    33961 ttggtaccaa taggcaaact cttcaacaag atacctc
//
LOCUS       DAOOMC010000010        33789 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdus sp. isolate AMR_MDS_2685
            NODE_1063_length_33789_cov_6.304500, whole genome shotgun sequence.
ACCESSION   DAOOMC010000010 DAOOMC010000000
VERSION     DAOOMC010000010.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864760
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdus sp. (wastewater metagenome)
  ORGANISM  Syntrophorhabdus sp.
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae; Syntrophorhabdus.
REFERENCE   1  (bases 1 to 33789)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 33789)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,729
            Genes (coding)                    :: 2,708
            CDSs (with protein)               :: 2,708
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 7 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33789
                     /organism="Syntrophorhabdus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1063_length_33789_cov_6.304500"
                     /isolate="AMR_MDS_2685"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2699756"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(738..1205)
                     /locus_tag="PK125_01835"
     CDS             complement(738..1205)
                     /locus_tag="PK125_01835"
                     /inference="COORDINATES: protein motif:HMM:NF013340.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
                     /protein_id="HPB36880.1"
                     /translation="MEIMSSAFKDQDKIPVQYVMPGAGGRNISVPLSWKNIPSGTKSF
                     ALSMVDPHPVAQNWVHWLMINIPANISSLEEGASRKIIPAGCMELRNSFGDIGYGGPQ
                     PPKGTGDHPYVITIYALNVEKLDLGVNTSLSVFEKALEGRSLGSARLTGKYGR"
     gene            1666..3477
                     /gene="iorA"
                     /locus_tag="PK125_01840"
     CDS             1666..3477
                     /gene="iorA"
                     /locus_tag="PK125_01840"
                     /EC_number="1.2.7.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR03336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043805 - indolepyruvate ferredoxin
                     oxidoreductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate ferredoxin oxidoreductase subunit
                     alpha"
                     /protein_id="HPB36881.1"
                     /translation="MKKEIMQGNAAIARGAWEAGCKVAAAYPGTPSSEILKDIADNYP
                     EIYAEWSPNEMVAMQVASGAAIAGARSMCSMKHVGMNVAADAFMTLSYTGIKGGMVII
                     VADDPNVHSSQNEQDSRNWARFGKAPMLEPADAQECKDFTKIAFEISEKFDTPVFVRT
                     ETRVAHADSPVTLEDRKESKISLGLDPKEATKYVMVPANVRVRRKAVEEKMKKLEEYA
                     ETFKYNVVEMNDTSVGVIASSAAYLYTKEVFPEWSYLKLGMVWPLPKKMITDFAKKVK
                     KLVVVEELDPFFETEIKAMGIKIWHGKDVIPNMYELSPEIVEAALKGKKYKAPKVRVK
                     PEDLPRRPPNLCAGCSHRPLFYALKKLGAFVFGDIGCYTLAFAPPLQALHTTICMGAG
                     GGQAHGASKALGKEGLGKVCAVLGDSTFLHSGLSPLMNAVYNKGNSTTIVLDNRITGM
                     TGHQEHPGSGFTVRQEPTNMVDYAEIGKALGVKSIRNIDPYNIKETMEVLKEEMAKDE
                     PSLILCKDSPCMLLRRAKPLERFKNPVYVINTDNCRGCKMCLEINCPAISWREGAGQT
                     KDGHKRKGTVYINPDQCVGCEVCVQICKYDAIEPGKK"
     gene            3489..4082
                     /locus_tag="PK125_01845"
     CDS             3489..4082
                     /locus_tag="PK125_01845"
                     /inference="COORDINATES: protein motif:HMM:NF013707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate oxidoreductase subunit beta"
                     /protein_id="HPB36882.1"
                     /translation="MKDNGKVTNIFLSGVGGQGTILASNILAEIFLKAGYDVKKAEVH
                     GMAQRGGDVTTHFRFGKKVYSSLIKYGDVDFLLSFEELEALRYINWVKPDGKVLINRQ
                     EIFPPAVSLGNMAYPSDVEKTFKKYFKDNVWMIDGQGIAKQLGNAQAANVVLVGAFSN
                     FFPAVKEEQWLDAIKGLLAPKLHDLNVKAFYAGKKAL"
     gene            4346..6802
                     /locus_tag="PK125_01850"
     CDS             4346..6802
                     /locus_tag="PK125_01850"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HPB36883.1"
                     /translation="METFTLDNRLPVIFETRKNTGVVAAQVWVKVGSKYEEPRIAGIT
                     HFIEHLIFKGTAEVKAGEMASRIESLGGSVNAFTSYDNTVYHIVVPTKSFEEGLGLLL
                     DAVKNPAFPETEITKEKRVVLEEIKMGEDDPHRKLFKELFSMSYEGHPYGRPIIGFEE
                     TVKNISREDILAYFHEHYTPDNMSVVITGDFDTVKAREIISKFVKGFGDEKTGVFAAT
                     RIKPETPAQKQKVIERSVRESYVSISYPVPSITHDDTVGLEVLAKILGDGDSSRLQDQ
                     LKFKKGMVTNISTYLFSPKEDGLFIIIATFKGQAFDNITKAMDEVIERLLQSGPTEEE
                     VEKAKNMIRASYIYSSETAQGAARLFGNYQTLTGDPRYPDKYLRALDKVSLSDITRLL
                     KKYLVGKERKLAALVPKQPLNPHIFTMKNGMSYVVNKNPSSPSFAFMIGFVGGLRDEP
                     TGKNGAFNILSRMLMKGTKTKNAAVIAKEIELLAGDISPYNGRNIFGLSGKFLAKDFH
                     KAFALLKELLTETALKEEELKKVKEDVLSSLRQRDDDPISFTFRRFTEGLYKGHPYSK
                     DPMGSEVDIQSITLKNVTDFYKQYVTPAKAVLAISGDIDEKTIQEAMDGLFKGWNGPA
                     NVLGKDSVVTRKHEVAVDRDMMQTHLIFGFLGPGLVDNDRYAVEVMDAVLSGMGGRIH
                     KVLREENPYAYALTFFNQMAFDFGGIGIYIGTDRKLVQEVSRIAKSEIQKMVKDGFSE
                     EEVNNAKSYLVGNHYISMQSNSSIAMSMCLDTIYGMKPGFFKIWPQHIEKVTLSDVNR
                     VARKYLNLDKMVSVRVGAGE"
     gene            complement(6945..8048)
                     /locus_tag="PK125_01855"
     CDS             complement(6945..8048)
                     /locus_tag="PK125_01855"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HPB36884.1"
                     /translation="MNIPPINLKVQFKSVRRDILRSIREILDEQKLILGKYCTTLEEM
                     IGCYVGVPHAIACANGTDALILSLMALDIRNGDEVITTPYTFFATASSIALMGAKPVF
                     VDIEPEDMNINPALIERAITPRTKAITVVHLFGKVCQMEKICDIGQKHGISIIEDMAQ
                     SLGAMKDDKMCGVFGDVAAISFYPTKNLGGIGEGGMVLSKRSDLGGKIRKLRVHGMAD
                     IPYHHEMIGINSRLDEIKACALVMKFSHLQKWNMTRLKNARYYNRMLQSLPILLPRIE
                     DESSHIFHQYVIRVEERDKLQTFLKEKGIQTGIYYPVPLHLQECFAHFGYARGDFPEA
                     EKAALTSLALPVYPELKKVEKDYIIRSVREFFS"
     gene            complement(8045..8611)
                     /locus_tag="PK125_01860"
     CDS             complement(8045..8611)
                     /locus_tag="PK125_01860"
                     /inference="COORDINATES: protein motif:HMM:NF013077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HPB36885.1"
                     /translation="MDIIKVIKERRSIRKYKKDAVPDEIVADIIDAARVAPSWANTQV
                     CQYIVIKNQKIKELLSETLIPATNPARAAVVDAPVIICLFAKRGVSGFYKGTPSTDKE
                     GYWFMFDAGIAMEHIVLAAWSFGLGTVHVGNFDAKKAEGVLNIPEGFSMVGITPLGYF
                     DEIPKQPSRKKLEEITHLDSFGQPFLKS"
     gene            complement(8782..10089)
                     /locus_tag="PK125_01865"
     CDS             complement(8782..10089)
                     /locus_tag="PK125_01865"
                     /inference="COORDINATES: protein motif:HMM:NF013696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HPB36886.1"
                     /translation="MHESDSAADLLREFIRIDTTNPPGNEEDAVRFLDECLDKEGIPT
                     EVFQVAPKRANLLARITGKSKGKPIILLSHIDVVPAKADEWEVPPFSGEVRDGFIYGR
                     GAIDMKSQAICQLLAFARLQKDGVKPERDIIYLATCDEEVGGQYGVEYMLKNVDELRS
                     ASFVLSEGGCIIDEGGCLHAQVSVAEKKLSQFTIRAKGTGGHGSMPHSDSANKKVLRA
                     ANAIISHQWPFKQTSIVSTYLNGILKNEKGKGFVFNNLKQALKNKQFRSFVSDNPVYN
                     ALLRNTVTPTILEGGEKVNVIPAESSISFDARLLPTQSHDHFFKKIEKLAGKDVKVVP
                     IGTGKSDPLTSGYNTQYFKGIKKAVINLIGPIPVLPFLTTGATDLRYFRNLGIPAYGF
                     FPITLTNEELFRMHGKNERISIVALKQGLIGTYEILKFLATVK"
     gene            complement(10082..11518)
                     /locus_tag="PK125_01870"
     CDS             complement(10082..11518)
                     /locus_tag="PK125_01870"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF013180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HPB36887.1"
                     /translation="MGGAIPHCFTMETRKRTFVIDDEEDILFILKEFFRNHGIEAFTY
                     EMVPDMASEIREKKPNAVLLDIFLPGISGIEILKKIKEIDSNLPVIMMTGHLDEEKKI
                     ESLRSGAYAILAKPFKSYEEVYHITNNAANHYLETQRTIELTAEIEKRHEHERLNLLE
                     LDFLKKLQLMIGETEDPAFVIKNSFALLKNFLNFQAFAALMLQGEDINIQIYPNVGSN
                     QKLMECITMSLMNKAPERTKVEKQMSEMTIAGKDTLSTGEDKYNFMTVDISARDRIYG
                     YAGLYRVLPFHDDEKAIFNRFCSNIAMTLEKISLFNEIRSLSIRDGLTGIYNHAFIVE
                     TLKDEVERSRRYGSLLSIALFDIDDFKKVNDTFGHLAGDAVLQKLAELLKSSLRAIDI
                     VGRYGGEEFLAILPETDGINGYIVAERFRERIEKESFTNDEGKFNITISGGVAHFTQG
                     MDVNKLIKIADDNLYAAKRGGKNRISYA"
     gene            complement(11772..12221)
                     /locus_tag="PK125_01875"
     CDS             complement(11772..12221)
                     /locus_tag="PK125_01875"
                     /inference="COORDINATES: protein motif:HMM:NF012241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HPB36888.1"
                     /translation="MALTPWRGLWESRFPSLRDEIDKMFEDFFGKTDFSSLADGTWNP
                     PMDVQQTKKDVIVMMDIPAIDPKAISISIIEDRITIKGERKREEESQEADYYRSERVY
                     GSFQRIIQLPAEVVGDKAKASYKDGVLKITIPKSQKVVPKEVKIEVQ"
     gene            complement(12238..12492)
                     /locus_tag="PK125_01880"
     CDS             complement(12238..12492)
                     /locus_tag="PK125_01880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB36889.1"
                     /translation="MEFEYTIKAYSMQQLSEKGVVVDPEKNIVYACRPDGACEIRDVG
                     VEQMVHLSDLFNEMGKEGWELVQLLFRPSGMVSFWKRNKK"
     gene            12636..13478
                     /locus_tag="PK125_01885"
     CDS             12636..13478
                     /locus_tag="PK125_01885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB36890.1"
                     /translation="MMHLNPCVLCPRKCKANRAAGELGYCRAPYHLYVSSASPHFGEE
                     SPLVGINGSGTIFLSHCNLRCIFCQNYDISIYGEGYPCTYNDLAATMVQLQERGCHNI
                     NFVTPTHYAPHLVRALSIAIDMGLSIPIVYNCGGYESLEVIKLLEGVIDIFMPDIKFL
                     DPALSSRFCLAENYPEIVKSVVKEMYRQVGNLSINADGTATRGLLIRHLVMPSCGEDT
                     KRILRFVQEEISQDAFVNIMAQYHPCNRAKEYPELSGRISGKEFSDALRYARELGLTR
                     ATNH"
     gene            complement(13468..13740)
                     /locus_tag="PK125_01890"
     CDS             complement(13468..13740)
                     /locus_tag="PK125_01890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB36891.1"
                     /translation="MRFFTIFVIVLMLISCIKGMDAPSQKKVSEAQGMDGQYQKELDE
                     IKKSLKGDIKIKLKRESKGGYSWEVTGKDAQEVLKANDAIRKKLSD"
     gene            13799..14533
                     /locus_tag="PK125_01895"
     CDS             13799..14533
                     /locus_tag="PK125_01895"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HPB36892.1"
                     /translation="MNLNVEVYGSGEPIIFVHGVGGSSQYWHYQKEYLKDLMKVILID
                     LPGHGKSSGSACETIEEARDIVREAIMELGIHKCYLAGHSMGGGIAISFALAYPELLR
                     GILLICTGAKLRVLPAILKEILENKEDTVRMIMMEYAFSNKAPQKMKENGFKDMMKSS
                     ATTIYKGFAACDKFNAIGSLDKITLPTMIIGGKDDLLTPPAFSEYLYKNIKGSELVIV
                     EDAGHMVMIEQSDTVNKAIKNFVFEG"
     gene            14616..15041
                     /locus_tag="PK125_01900"
     CDS             14616..15041
                     /locus_tag="PK125_01900"
                     /inference="COORDINATES: protein motif:HMM:NF012684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor-like protein"
                     /protein_id="HPB36893.1"
                     /translation="MKFRTVVNREKEKAILEKMEHLGIREEDIVERFIRSRGHGGQNV
                     NKVSTCVYLKHVPTGIEVKCQQERSQALNRFLARRILIQKIEDQTLGRESEKQQHIAK
                     IKRQKRKRSKRAQEKVLHLKHLQSEKKRARSHIPDPWEY"
     gene            15242..17899
                     /locus_tag="PK125_01905"
     CDS             15242..17899
                     /locus_tag="PK125_01905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB36894.1"
                     /translation="MAIHSHSDTKPLTKPGSIFADAYPDSLLEIFAILVARFENRKNE
                     SDSSEKQPEEGLLPDLIAEASSLLATHGGTVIQSSDGALQAYFHIGKNAVKAAIALRH
                     ALGIYNASKNLQSPFHVKIGVDYGEIIRDTDNLHGNTVENAQLLSDIASPDEICVSWP
                     VFNLSKDLPAVYFEMVHPWNRKKIPEGFEIYRIVWNPTQKTSCLSCVILCFRPIWKLW
                     DQTFADIWEDLISNGQTLWGGHSDQKEILDDRSIVLILKSMESILPLSMAVSKFLRKR
                     IQNSTTSLIPIQIIADIGPYGENWQARTNGLPPLWEKLNPGYLYISEQTYASLQQKIE
                     IPGNAIHRTYSGQSFYQVGLDNELPSQETKRFLYQKALVQGDLLPCFYCGDRKHHPTK
                     CPSKNIPENTGALNQLGYCSIDELNDIFYHHIIGENGPGVNPQVEDSLSLPASGFFEL
                     KRVFQLRFFRSFWNTTNEEWNNIRKSRNKSEGGLIWLAQDSLRVSELTKAESMLVSAM
                     NKYPLDYRVYVASGFLDIEKGDLSRAEHDFSEAYAIAKTNVQKAFTLLLLSRLYWITG
                     NLDKAYEKVQRIYSLNVDSVDAMYQDVLLKLFQGKEKLACQRLSRLVQEHREFFVAAM
                     IDPDLAPYSGIVGDTLEIILNRARQDAQSAVSDADNEYELSKVVLGKSDLNDIQILQT
                     KIEQLLNKDSYCGYLDIIDHCNSIITTCRKRTIHRRREIWGILRELNKRLETNMMFVE
                     SYPYKSMVRSCRQQLVHSSEKIRHVQSIGPALSQEQLAACHSLYEELTKEWDSLESKL
                     RGLNCFLQFCKASLHFLRWSGTFMAITWFLGLFLFPLIIYYLNAFLSGFATLTFSNVW
                     FYQKNCVLFGSLAGIGVALAITIKSLFKK"
     gene            complement(17933..18169)
                     /locus_tag="PK125_01910"
     CDS             complement(17933..18169)
                     /locus_tag="PK125_01910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB36895.1"
                     /translation="MKIKNRVCYIVVMPKEEDKQQVKCVDCIHFYITWDKYYPKGCKA
                     MGFKSAGMPSTLVHKTSGTKCLRFEMKQTRGKRR"
     gene            18249..18878
                     /locus_tag="PK125_01915"
     CDS             18249..18878
                     /locus_tag="PK125_01915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB36896.1"
                     /translation="MDLMALAELLLVSLYDLSEEQPHSYFFFQLDQIGTRVGATDMEQ
                     LTKAAHILEDRGFAMLSASPLSILSAMISSDGISFVESGGETGIIKTYRNNPREVIDI
                     VCKEVQAPPESVSNSIQEDIPATVQALSLNETMQTILSRIGSVLADDSSIITTVREDA
                     IKDLESLKIQLSRSSQNWRLVEMLLDALAEIPSLLPSLKDLALVLRRLE"
     gene            18932..19369
                     /locus_tag="PK125_01920"
     CDS             18932..19369
                     /locus_tag="PK125_01920"
                     /inference="COORDINATES: protein motif:HMM:NF024859.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HPB36897.1"
                     /translation="MAFKLDHIGLVVENIDKMKELFGTIGLDTGTEAIVNPLQKVSAS
                     FVNVGEKDDIYIEILEPASNDSPISKFLQNRGGGLHHLCFEVDNIEDAIKELNSKGFR
                     MVVPPEDCRAYDENLKRRCQEPTKIAFFIVTKGVLIELIEKGG"
     gene            19580..20785
                     /locus_tag="PK125_01925"
     CDS             19580..20785
                     /locus_tag="PK125_01925"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPB36898.1"
                     /translation="MLLLGFASGLPLPLTGGTLQAWLTIAGVDLTTIGIFSLVGVPYI
                     IKFMWAPLMDRFVPTWLGRRRGWIFPIQLGLFFGIGLMGFTQPSCAPLMLGVLAFLVA
                     FTSASQDIVIDAYRTDILYEKERGIGAATFVLGYRVAMLVAGALALIISDHIGWHKTY
                     LIMALVMIAGMVGTFTGPEPKENTASPKSLKEAVIGPLQDFMAHPYAFSMILLIVLYK
                     LGDAYAGTMTTTFLLRGVGFSATEVGTINKGMGLVATIVGAMFGGTLMVKLGLFRSLM
                     MFGFFQMVSNLSFMALAWFGKSYELMIFAVAFENLCGGMGTSAFVALLMALCNKRYSA
                     TQFALLSSLSAFGRVLISPSSGYVINITGWAMFFFITTLAALPGLWLLWRLRNQIDNL
                     KQGQSIDNS"
     gene            complement(20827..21522)
                     /locus_tag="PK125_01930"
     CDS             complement(20827..21522)
                     /locus_tag="PK125_01930"
                     /inference="COORDINATES: protein motif:HMM:NF018963.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1460 domain-containing protein"
                     /protein_id="HPB36899.1"
                     /translation="MIAKAAQIQSTGRRVEYISRQFLGVSYRGKTLIGDDKTPERFVV
                     DLSGVDCFTFIDYVEALCFADSFLSFQESLICTRYRSGIVTFDHRNHFFTDWIVANRT
                     YIEEVTKKVGGRYTMFSKKILNLKENGAFYVPCIKPVERTIDYIPAKSIENIVLKNCR
                     TGDYIGIYADNPGLDVTHVGIFVRKKGTPHFRHASPVQGKVIDQEFEGYMVDKPGFLV
                     LRPRRVQTTSRKE"
     gene            21667..22584
                     /locus_tag="PK125_01935"
     CDS             21667..22584
                     /locus_tag="PK125_01935"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HPB36900.1"
                     /translation="MRIVDVHTHGLAGYSTNTMNPDEILEVARLHGMHGVTDIILTIY
                     PGPVEIMKEHMTAVEEAIKRQRLAISSQGSVRAESEHETQNPKLARILGIHLEGPFLN
                     PSKAGALDASSFLKPSEKIWESLIEGFEGIVRIVTIAPELEGAKTLIKAISNKGIIVS
                     LGHSEATYEETENAFHAGAKGITHLFNAMRLFHHREPGIAGFGLMNPHIYVEVIADPS
                     HLNRRTIEFIFNVKNHEKIVIISDSIKATKVGAGQHALMNSARILEGGSMTVTESASR
                     LIDMGFDEAKIIGCISTNPLSYLALTKQC"
     gene            complement(22602..23081)
                     /locus_tag="PK125_01940"
     CDS             complement(22602..23081)
                     /locus_tag="PK125_01940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB36901.1"
                     /translation="MKWLLNKRFAANIILLIIGAGIVFFYTLCGDSCAYLQGTIFGIE
                     LQYLGIVYVVILIFFNILKNGLIVLVLLSAGIGVEVFLIGYQVMNSTYCSYCLVFAAL
                     IFFQFILNLDWSRKLLILVFMVLGFFCFFIFFEGSAFPVYSRTINLFVQTVPAIKTV"
     gene            23483..23683
                     /locus_tag="PK125_01945"
     CDS             23483..23683
                     /locus_tag="PK125_01945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB36902.1"
                     /translation="MFVKDNEAGVIIDYLAKKLREMGMGYRQRKKPLIAERFWPALRS
                     KNQNSIDFHLYSIALRQLSWEQ"
     gene            complement(23666..24292)
                     /locus_tag="PK125_01950"
     CDS             complement(23666..24292)
                     /locus_tag="PK125_01950"
                     /inference="COORDINATES: protein motif:HMM:NF014244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HPB36903.1"
                     /translation="MGLINLLFKDPVAFVLVAIPLLYSIIIHELAHGWVADKMGDSTA
                     RWLGRLSLNPVKHLDPMGTIMLFIFGFGWAKPVPVNFNRLRDFRKGLIFVSAAGVTAN
                     IILAFIALFLLQLLSRWHIESLNTLLFYLAQINIMLAAFNLIPIPPLDGSKILMGFSS
                     RSFQYSLARLEPYGFFIIIGLLWLGALNPLIAFFRMIIVGFIKLLLPG"
     gene            24530..24910
                     /locus_tag="PK125_01955"
     CDS             24530..24910
                     /locus_tag="PK125_01955"
                     /inference="COORDINATES: protein motif:HMM:NF014176.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HPB36904.1"
                     /translation="MKKGIVLLFTLTVFLFGIFSITGLVFADKQPPDSIVIKNEGAKL
                     PPVNFSHKSHVEKNKLECVKCHHKDTNPKEPERCVKCHLISEVKDNAPIAKDAYHKQC
                     QTCHKDSSAKGVKAPTACNECHKK"
     gene            24891..26207
                     /locus_tag="PK125_01960"
     CDS             24891..26207
                     /locus_tag="PK125_01960"
                     /inference="COORDINATES: protein motif:HMM:NF016995.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein ResB"
                     /protein_id="HPB36905.1"
                     /translation="MNAIKNKAAEQVTPPGKSLFQSLQGFIASIKFSIILLTCIALGS
                     IIGTIIKQGVGPDEYLSLYSEHTYQIINLLGLNDVYHSPWFIALLFLFAINLTLCTVR
                     RFMPAFKGSKKASVPEESAMMDMSMSFRASTSIDDAHLFSILKGYRISYSGEEGKVFQ
                     KGALSKYGVLLIHTSIMLILIGGFVGLVAGFKGFMVLHKGDTKNTITIGGEKPYEKPL
                     GFSIKCKEFQVSFYPNGAPKDYVSTVEVIENGKIIFEKQIRVNEPLSYKGIHIYQASY
                     GSAPSFTFTIGGERVSLREKEAYRNDNISLMPIRFEQDIHNFGPGVMVAYLEQGEPRT
                     VWFVKNIERFNEKIIQGVPVRFVDIQENIYTGLSVSRDPGIWIVWAGFAFILFGLYIN
                     FFQYYRRIYIRNIAGGYLVAGIAIKNKDAFKKEFEMLREKLNGIPS"
     gene            26191..27006
                     /gene="ccsB"
                     /locus_tag="PK125_01965"
     CDS             26191..27006
                     /gene="ccsB"
                     /locus_tag="PK125_01965"
                     /inference="COORDINATES: protein motif:HMM:TIGR03144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome biogenesis protein CcsB"
                     /protein_id="HPB36906.1"
                     /translation="MAFHHKILGIVTLLYLCLFFIHLIFFAFKKEKIVAIMWPLLYGT
                     LGLHTVGLILRWLESYRHGMGHAPLSNFYESLVFYAWCVSLIVVFMKKRLNYPAITIC
                     ATVISLFLMGYASISPSVDRNIQPLVPALQSNWLHIHVLTCFLAYASFAVSFISGFLY
                     MFTWKNVIPPRDILDEINYRSVVVGFPMLSAGIFTGAVWAHYAWGSYWSWDPKETWSL
                     ITWIVYAVFLHARLMRGWKGKKIAILSIVGFASVIFTYFGVNFILSGLHSYAT"
     gene            27053..27874
                     /locus_tag="PK125_01970"
     CDS             27053..27874
                     /locus_tag="PK125_01970"
                     /inference="COORDINATES: protein motif:HMM:NF024897.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HPB36907.1"
                     /translation="MKKRKPKSLILVGNGINTEYETAHAHRLVGFEPDFIHINTLIED
                     PHRIKGYTFITLPGGFLDGDDLGSAKAQAVRWKYRKINKTGKTFIDLLISFVQEGNII
                     LGICNGFQLLVKTGLLPGLGSEHSTQRVTLTFNDLGRFEDRWVYLKNNTFSHCIFTKD
                     IHTIYLPVRHGEGKFVVDTEKTLERIKQGGHIVMQYADDSGNVTSQYPFNPNGSIESI
                     AGLCDSTGRIFGLMPHPEAFVHRIQHPRWTREALPEEGEGLMIFKNAFKYVTENR"
     gene            27887..28165
                     /locus_tag="PK125_01975"
     CDS             27887..28165
                     /locus_tag="PK125_01975"
                     /inference="COORDINATES: protein motif:HMM:NF012442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HPB36908.1"
                     /translation="MNKMDVINKLGEDLRLNQKIAKIAVDTIIDTIKNAIINGERVEI
                     RGFGSFSLRNYKSYTGRNPKSGEVVHVEPKRLPYFKVGKELKDMIWKP"
     gene            complement(28266..29300)
                     /locus_tag="PK125_01980"
     CDS             complement(28266..29300)
                     /locus_tag="PK125_01980"
                     /inference="COORDINATES: protein motif:HMM:NF013046.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase"
                     /protein_id="HPB36909.1"
                     /translation="MNVGIVRDEIFLEHITDDYHPENPNRLKYIYTMLNSIDKEGITY
                     TPPRVATHDEIALIHDHAYIASIAATEGKMQRRLDPDTVTSPKSYEAACLAAGGVLQL
                     ADMLFSSKIQNGFALVRPPGHHAERNRAMGFCLFNNIAIGARYLEKRYGLKRIVIVDF
                     DLHHGNGTQHSFYGDSSILYFSTHQYPYYPGTGWYNETGEGNGKGYTINIPLSHGMGD
                     DDYEYVFREVLIPITDQYKPEAVLVSAGFDSYYNDPLGGMSVTEGGFATMTKILLEIA
                     DKHCNGKVICALEGGYDLNGLATSVKAVTMELKGTSMFTPDKKINPSNEIQETVDKVK
                     QVLRPFWGEL"
     gene            29659..31371
                     /gene="pilB"
                     /locus_tag="PK125_01985"
     CDS             29659..31371
                     /gene="pilB"
                     /locus_tag="PK125_01985"
                     /inference="COORDINATES: protein motif:HMM:TIGR02538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV-A pilus assembly ATPase PilB"
                     /protein_id="HPB36910.1"
                     /translation="MPLRLGDLLVKMSRITEQQLIEALKVQKNEGGKLGEIIIKMNFI
                     TEKGILEALSDQFGIPQINLDQTEINPDVIPLIPLNTIRRFHIMPIRKMNNMLTIAIS
                     DPTKAFDLKEIKLLTGLDIEPLLASENAISKAIETHYETNHAAELRKFMDGLGVGADA
                     SLEIVEEEEKGDITKLESDAKLPPVVQIVNHIFTEAIAKGASDIHIEPYEDRARVRYR
                     IDGLLYEALTLPLKYKDGVISRVKVLTKMDIAEKRLPQDGRIKIQMKLSGRMKNLDIR
                     VSTTPTIFGEKVVMRLLDREGLMLDMAKLGFEPESLRKFEEAIFKPWGMILVTGPTGS
                     GKTNTLYSAIGRINSPDTNIMTVEDPVEFNLGGINQVQVHESIDLTFASVLRSFLRQD
                     PNIILVGEIRDNETADIAVKASLTGHLVLSTLHTNDAPSTIIRLIDMGIDSYLVATSV
                     ILIAAQRLVRRICSACKEEINIPPKALLNIGFSEEDAATVKIFKGKGCPKCNNTGYKG
                     RVGLFEVMQVSPAVRDMILSDASVADIRKQCIDEGMLTLRQSGLAKIRNGVTTIEEVI
                     RETF"
     gene            complement(31457..32257)
                     /locus_tag="PK125_01990"
     CDS             complement(31457..32257)
                     /locus_tag="PK125_01990"
                     /inference="COORDINATES: protein motif:HMM:NF003969.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative hydro-lyase"
                     /protein_id="HPB36911.1"
                     /translation="MVLIKDITSLKPDKIRAVIRSGKWDKPTTGIALGYAQANLVILP
                     KKYALDFFLFCQRNPKPCPLLEVLEPGDFRPDFLASEADVRTDAPRYNVYVKGDLKAS
                     PTSVKELWRKDFVTFFLGCSFSFEEAIIRSGIPIRHIEEGKNVPMYLTSIKCRPAGIF
                     HGPMVVTMRPVNPAEVVKVVQITSRYAFVHGGPVHIGDPEIIGIKNLEKPDFGDPVTI
                     KEGEIPVFWACGVTPQAAAMEARPNICITHAPGHMFITDILNEKLATT"
     gene            complement(32296..33000)
                     /locus_tag="PK125_01995"
     CDS             complement(32296..33000)
                     /locus_tag="PK125_01995"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPB36912.1"
                     /translation="MLKVVKVEVRYGGIPIIHDVSLEVKQGELVAVVGSNGAGKSTLL
                     KTIAGALHPTKGSITFDDVLINRLATPDIVRLGITYIPEARLIFGPLSVEENLKLGAH
                     IIKDHAVISRNLDFVYDLFPRLKERRQQASGTLSGGEQQMLAIGRGLMSNPKLLMLDE
                     PSLGLMPKLVDEMLEAVAKLRTIGMTILLVEQNVFESLEIADRGYVLQTGRTILDGTG
                     KELLNTESIKKAFLGL"
     gene            complement(32984..33715)
                     /locus_tag="PK125_02000"
     CDS             complement(32984..33715)
                     /locus_tag="PK125_02000"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPB36913.1"
                     /translation="MAFLEVRDIVKDFGGLVANSNVSFQIEEGEIVGLIGPNGAGKTT
                     LFNCIVGYYSPDYGKVMFKGNDITGFKPFQTNQAGIGRTFQIMKVTTGMTVVENIMVG
                     AFCRTNSRHQAREEAHEMLHFLGMEEISEQYINELPIAKQRKVGMARALATKPQLLML
                     DEVASGLNHTETDEMVALLKRLNVEKKITLFLIEHIMEMVMTVSQRVIVLDGGIKIAE
                     GVPGDIVKDDNVIKAYLGEKYAKGR"
BASE COUNT         9853 a         7382 c         7187 g         9367 t
ORIGIN      
        1 tggaaaaaat tattatgtta ttatggtgcc ataatgaccg gtattcttta aacaaaccat
       61 cgggaagcaa agaagcttgg ccttttgagt caacaaagca tctgatgcta aaggcaacca
      121 agggtctcca ctgagattac tgagcctgtt tttttgaata atgactaact ccacacccat
      181 ctcatctgtg gaaattgtag gagacatttg tataatacgg gtgccttcta taccaatatg
      241 agacactttc cattcaatag cttaaacttt aagaacagga aggaaagtga ccatggaaaa
      301 accatacagc aagaatgtgt ggaaagagat cagggtcacc aagaaccatt cacaaccttt
      361 gatgagtaat aacaactccc tattcagaat aatagttcaa gactatgaag tgtgatccgg
      421 aattttctga gaggttcggc tctatcgaac atgttagacc atttttcaac cttcttttcc
      481 tggcacaata ctgaacatca tctttctggg ttagggcatc acaaacccgg acatgtccat
      541 tatggaaggg ttccccgtat tcttgaagtc catcagaaga tagttcattc tacacacgaa
      601 caacatccat tttagttata gtaaggcaag gtcatgaatc ggtcttttgc cattttgaaa
      661 aggaatcaca gcttatgcac agttactcat acgcactttc aaacattaat gtattgacat
      721 gttgtatttt tcgcccctta acgtccatac ttaccagtga gccttgctga ccccagactc
      781 cttccttcca aggctttctc aaataccgat aacgatgtat tcacccccaa atcaagtttc
      841 tccacgttta acgcgtagat agtaattaca tagggatggt cgccagtacc ttttgggggt
      901 tgtggacccc catagccaat gtccccaaat gagttcctca attccatgca acctgccggg
      961 attatctttc ttgatgcccc ttcttcgaga gaagatatat tcgcaggtat attgatcata
     1021 agccaatgaa cccagttttg tgccacaggg tgtggatcaa ccatagaaag ggcaaaggac
     1081 tttgttcctg aagggatgtt tttccaggag agaggaactg aaatattcct gccacctgct
     1141 cctggcatga catattgcac tggaatcttg tcctgatctt taaacgctga tgacataatt
     1201 tccatttttg aacctcctga tgaatcttct gcctgcagca agagcaaaac gatcaaaacc
     1261 aacaacctga caaatttgtt aagtttcaag gaaaatcacc tccagatgaa atagcaccca
     1321 aagctttctg gggatgcaat caccgtatat cgtaattata acagctttta ataggtttga
     1381 cagtgtattc gtccagtgtg tcctacgata tatcccgttt tttaaattgt atactgaaaa
     1441 ctctttgatc tgcctttctt cttaccttgc aacaacttgt ttttaaaaaa attatggtta
     1501 tatgaaattt cttgacaaac actctgattc ggtggaaaat aatgaaaatg taataagtga
     1561 attctggtac aagccacgaa aagatgctca gcaaatattt aaaagtacaa ggaggtgaat
     1621 gatttacatt aaaaatacca ggcattatgc cattatgggg gaaacatgaa aaaagagatt
     1681 atgcaaggaa atgccgcaat tgcacgcggc gcctgggagg caggctgcaa ggtagcagca
     1741 gcatatccag gcacaccaag tagcgaaatt ttaaaggata tagcagacaa ttaccccgag
     1801 atctatgcgg aatggtcgcc aaatgaaatg gttgccatgc aagtagcaag cggcgcagca
     1861 atagcagggg cacggtctat gtgttccatg aagcacgttg gtatgaatgt agctgccgat
     1921 gcattcatga cactgtctta tacaggcata aaaggtggaa tggttataat cgttgccgat
     1981 gaccctaacg tccacagctc tcagaatgag caggatagcc gcaactgggc acgatttggc
     2041 aaggcaccca tgctggagcc tgctgacgcc caggagtgca aagattttac gaaaatagca
     2101 ttcgaaatca gcgagaagtt cgacacaccg gtctttgtac ggacagagac aagggtcgcc
     2161 catgctgact cgcctgtaac ccttgaagac cgaaaggaat ccaagatttc acttggcctc
     2221 gacccaaagg aggcaacaaa gtatgttatg gttcctgcga acgtccgggt acggcgcaaa
     2281 gcagtggaag agaagatgaa aaaacttgaa gagtacgctg agaccttcaa gtataatgtt
     2341 gtggaaatga acgatacaag tgtgggcgtc attgcgagca gtgctgccta cctctataca
     2401 aaggaagttt tcccggaatg gtcctacctg aagctcggaa tggtctggcc tctcccaaag
     2461 aaaatgatca cagatttcgc caaaaaggtt aaaaaacttg tggttgtaga agagcttgat
     2521 cccttcttcg aaacagaaat caaagccatg ggcattaaaa tatggcacgg caaagatgtc
     2581 atcccgaata tgtacgagct ctctccggaa atcgtggaag cagctttaaa aggcaagaaa
     2641 tataaggcac caaaggtcag ggtaaaacct gaagatctgc cgcgaagacc gccaaacctc
     2701 tgcgctggtt gttcgcacag gccacttttt tacgccttga agaagctcgg tgcttttgtc
     2761 tttggcgata ttggttgtta cacactcgcg tttgctccac cgctccaggc acttcacaca
     2821 actatctgta tgggagccgg tggcggtcag gcacatggcg cctccaaagc attaggtaaa
     2881 gaggggctcg gaaaggtatg tgctgttctc ggcgactcca cattccttca ctccggcttg
     2941 tcacctctca tgaatgcagt ttacaacaaa ggcaattcta ctacaatcgt tctcgacaac
     3001 aggatcaccg gtatgacagg tcaccaggaa caccctggat caggctttac ggtacgtcag
     3061 gaaccgacaa atatggtcga ctatgccgag atcggaaagg ctcttggtgt aaaatccatt
     3121 cgcaacatcg atccatacaa cataaaagag accatggaag ttctcaaaga agagatggca
     3181 aaggatgagc cttcactgat cctctgcaag gacagccctt gtatgctgct gcggagagcc
     3241 aagccccttg agaggtttaa gaaccccgtc tatgtcatta atacggataa ctgccgtggc
     3301 tgcaaaatgt gtcttgaaat taactgtcct gctataagct ggcgcgaagg ggctgggcag
     3361 acaaaagatg gccacaagag gaagggaacg gtctatatca atcctgacca gtgcgtgggt
     3421 tgtgaggtct gcgtacagat ctgtaaatac gacgccattg aaccgggcaa gaagtgagga
     3481 ggtcgacgat gaaagataat ggaaaagtca caaatatatt tctctccggc gtgggtggac
     3541 agggtaccat ccttgccagc aacatccttg cggagatctt cctcaaggcc gggtacgatg
     3601 tgaaaaaggc tgaggttcac ggtatggctc agagaggtgg ggatgttacg acccatttca
     3661 gattcggtaa gaaggtttat tcatccctaa tcaaatatgg cgacgtggat ttcctccttt
     3721 ctttcgaaga actcgaagcg cttcgctaca taaactgggt aaagccagac gggaaggtcc
     3781 tcatcaacag acaggaaatc tttcctcctg cagtaagcct tggaaatatg gcatatccct
     3841 ctgacgttga aaaaacgttt aagaaatatt ttaaagataa cgtctggatg atcgacgggc
     3901 agggaatagc caagcagtta ggcaatgctc aggcggcaaa cgttgttctg gtaggtgcct
     3961 tctccaactt cttccccgct gtcaaagaag aacagtggtt ggatgcgatt aagggacttc
     4021 tcgcaccaaa actccatgat ctcaacgtca aagcgtttta tgctgggaaa aaggcactgt
     4081 aatttaggct tttgactacc aaaagggaag gcctgggcct tccctttttt ttctttttgt
     4141 tgattatcgt ctgtcaataa atgataatat gtatcctgcc tgacatcttg tcctttcttc
     4201 aaaaaaaggg ttgagcacag gaagcacaag tctgtcagag atccaagaaa cggtaggcac
     4261 aggcccttac tatagaatga gaaaactttt tttttactac gttgtactgt tttacatttt
     4321 cataaccttt ggaaaggtgt gtgctatgga aacctttaca ttagacaacc ggttaccggt
     4381 aatattcgaa acaagaaaaa acacaggtgt tgttgctgca caggtatggg tcaaagtggg
     4441 gagcaaatat gaagaacccc gaattgcagg tattacccat ttcattgaac acctgatttt
     4501 taagggaact gcagaagtga aggcaggcga aatggcctcc aggatcgaat cactgggagg
     4561 cagtgtaaac gcttttacct cttatgacaa cacggtctat cacatagttg tgcctacgaa
     4621 atcttttgaa gagggccttg gcctcctcct tgacgcagta aaaaatcctg cctttccaga
     4681 gacagagatt accaaagaaa aacgggtcgt tcttgaagag attaagatgg gcgaagatga
     4741 tccgcatcgt aagcttttta aagaactctt ttccatgagc tatgaggggc atccttatgg
     4801 aagaccaatt attggatttg aagagacggt aaaaaatata tcgcgtgagg atatactcgc
     4861 ttattttcat gaacattaca cgcctgacaa tatgtcagtt gtaattacgg gtgactttga
     4921 tacagtaaaa gcacgtgaga tcatctcaaa attcgtaaaa gggtttggtg atgaaaaaac
     4981 cggagtgttt gcggcaacgc gaattaagcc cgaaacacca gcacaaaagc agaaagtcat
     5041 tgagagaagc gtacgggaaa gctacgtttc gatttcttac ccggttcctt ccattaccca
     5101 tgatgatact gtgggtctag aggtccttgc taaaatcctg ggcgatggag acagctcgcg
     5161 ccttcaagat cagctcaagt ttaaaaaagg tatggtaacc aatatatcca cctacctctt
     5221 cagccctaaa gaggatggtc tctttattat tattgccaca tttaaggggc aggcttttga
     5281 caacataacg aaggctatgg atgaagtgat agaacgactc ttacaaagcg gccctacaga
     5341 agaagaggtg gaaaaagcaa agaacatgat tcgggcatca tacatataca gcagcgaaac
     5401 tgcccagggg gctgcacggc tttttgggaa ttatcaaacc ctaacagggg atccacgata
     5461 tcctgacaaa tacctcagag cgctcgacaa agtgtctctt tccgatatca cgagattact
     5521 caaaaaatat cttgttggaa aggagaggaa gctggctgcc cttgtgccaa agcaacccct
     5581 gaaccctcat atcttcacaa tgaaaaacgg gatgagttat gtggtaaata aaaatccgtc
     5641 atctcccagt tttgctttta tgatcggttt cgttggaggt cttcgcgatg agcccacggg
     5701 caaaaacggg gctttcaaca tcctgtcaag gatgctcatg aagggtacaa agacaaaaaa
     5761 tgctgcagtc atagcaaaag agatcgaact tctcgcagga gatatttcac catacaacgg
     5821 gagaaacatc ttcggtttaa gcggcaaatt ccttgcaaaa gattttcaca aggcatttgc
     5881 cctgttaaag gagcttctta cagaaacagc cctcaaggag gaggagttaa agaaggtcaa
     5941 ggaagatgtt ctttccagct tgcggcagcg agatgatgac cccataagtt tcacctttcg
     6001 acgatttacc gaaggactgt acaagggaca cccatacagc aaagatccta tgggttccga
     6061 ggtagacatt caaagcataa cactcaaaaa tgtaactgat ttttataagc aatacgtaac
     6121 acctgcaaag gctgtccttg ctatatcagg tgacatagac gaaaaaacga tacaggaagc
     6181 tatggatgga cttttcaagg gatggaatgg accggctaac gttttgggaa aggattcagt
     6241 agtcaccaga aaacacgagg tagccgttga cagggatatg atgcaaaccc atctgatctt
     6301 cggcttcctg ggtcctggcc tcgttgataa tgacagatat gctgttgaag tcatggatgc
     6361 agttctctct ggaatgggcg gtcgtattca taaggtgctc cgtgaagaaa acccttatgc
     6421 atacgccctt acattcttta accagatggc ttttgatttc ggcggtattg gcatctacat
     6481 cggcactgac cggaagcttg ttcaggaagt cagccgtatt gcaaaatctg agattcaaaa
     6541 gatggtcaag gatgggtttt ccgaggagga agtgaacaac gcaaaaagct acctggttgg
     6601 taaccactat atatcgatgc agtctaacag ctctatagcg atgagtatgt gccttgacac
     6661 aatatatggg atgaagcctg gtttcttcaa aatctggcct caacacattg agaaagtaac
     6721 acttagtgat gtcaaccgtg tggccagaaa gtatctgaat ctcgacaaga tggtttctgt
     6781 gcgggtgggg gcaggagaat aaaccagctc aatatagaat gaagaaacga taggcaaaaa
     6841 agcggaaagt ggagcccata aggcagacgg catggagaaa aaaacttgcc attaacttaa
     6901 ttattattga cgatctctgt taagaggcca aacagaattg atcgtcagga aaagaattcc
     6961 cttaccgacc ggataatata gtccttttcg acttttttta gttctggata cactggtaac
     7021 gctaggctag taagggcagc tttttccgct tctggaaaat cacccctcgc ataaccaaag
     7081 tgggcaaaac actcctgaag gtgtagaggc accgggtaat aaataccggt ctgaatccct
     7141 ttctctttga ggaatgtctg tagtttgtct ctctcttcaa ccctgatcac atactggtgg
     7201 aaaatgtgtg atgattcgtc ttcaatcctc gggagcagga ttggcaatga ttgcagcatc
     7261 ctattgtaat accgggcatt tttgagcctt gtcatgttcc acttctggag atgtgaaaac
     7321 ttcattacaa gggcacaggc ctttatttcg tccagtctgc tattgatacc aatcatctca
     7381 tgatgataag ggatatcggc catcccgtga accctgagct ttcgtatctt tcctccaagg
     7441 tcgctccgtt tggaaagaac cataccgcct tcaccaatgc caccaaggtt cttggtgggg
     7501 tagaagctta ttgcagcaac atcaccaaaa accccgcaca ttttgtcgtc cttcatggcg
     7561 cccaaagatt gagccatatc ctctataatg gaaattccgt gtttctgtcc tatgtcgcag
     7621 attttttcca tctgacaaac cttaccgaaa agatgaacaa cggtaatcgc cttggttcgc
     7681 ggggtgattg ctctctcaat aagggctggg ttgatattca tgtcttcagg ctctatatca
     7741 acaaaaacag gtttggcgcc catcaacgca atagagcttg ctgttgcaaa aaaagtgtaa
     7801 ggagtggtga tcacttcatc gccattccta atgtccaggg ccataaggga aaggataagg
     7861 gcatctgttc catttgcaca ggcaatggca tgggggacac cgacgtagca tccaatcatt
     7921 tcttccaggg ttgtgcagta ttttcccagg atcagttttt gttcatccag tatctccctg
     7981 atggagcgca ggatatcgcg ccgaacagac ttgaactgta ccttcaaatt tatcggtgga
     8041 atattcatga tttcaagaat ggttgcccaa acgaatccaa gtgggtaatc tcttcaagtt
     8101 tttttcttga cggttgtttg ggaatttcat caaaatagcc caggggcgta atccccacca
     8161 tgctgaatcc ctcaggtata ttcaacacac cttctgcttt ttttgcatcg aagtttccca
     8221 cgtggactgt acccaaccca aagctccagg ccgcaaggac aatatgctcc atggcaatcc
     8281 ctgcatcaaa catgaaccag tacccctctt tatctgttga cggtgtaccc ttgtaaaaac
     8341 cggacacgcc cctttttgca aagaggcata tgattaccgg cgcatccacc actgccgccc
     8401 ttgcagggtt agtagcaggt attagcgttt ctgacaggag ctctttaatt ttttgatttt
     8461 taatcacaat gtactggcag acctgtgtat ttgcccatga tggcgcaaca cgcgcagcgt
     8521 ctatgatatc agcgactatc tcatccggaa cagcatcttt tttgtacttt ctaatgcttc
     8581 tccgttcttt gataaccttt ataatatcca tgcaactacc tccccggcgt caatatagca
     8641 catcctcccc actcactcaa taaacaaatg aaacaaggtt atggaaatgg gacatagggc
     8701 taagggacca gataaagtga gaagcaaatt acggttaatt aaagttttcg ctactatgag
     8761 ctgtgagcta aacgctataa gctacttgac tgttgcaagg aatttcaaaa tctcgtaggt
     8821 gccaatgagt ccttgtttga gggcaacgat cgatatacgc tcatttttcc catgcatacg
     8881 aaaaagttct tcgttggtga gggtaatagg gaaaaatcca taggcaggaa tgccaaggtt
     8941 tcgaaaataa cgaagatccg tggcacctgt tgtgagaaaa ggcagcacag gaattggtcc
     9001 tataaggttt atgacagctt ttttaatccc tttgaaatac tgtgtattat aaccggaagt
     9061 taatggatcg cttttgccgg taccaattgg aacgaccttc acatctttac ctgcaagctt
     9121 ctcaatcttc ttaaagaaat ggtcatgact ctgtgtcggc aaaagccggg catcgaatga
     9181 aatagaagat tctgctggta ttacatttac cttctcacca ccttccagga tagttggggt
     9241 cacagtattg cggagcaacg cattataaac tggattatct gatacgaaag acctgaactg
     9301 cttatttttc agggcttgtt tgaggttgtt aaaaacaaaa cccttgccct tttcattttt
     9361 cagaataccg ttgaggtagg tacttacaat gcttgtctgt ttgaaaggcc actgatgtga
     9421 aatgattgca ttggcagcac ggagcacctt cttattggcg ctatcactgt gaggcattga
     9481 tccatggccg cctgttcctt ttgctctgat cgtaaattgg ctcagctttt tttcagctac
     9541 cgatacctgt gcgtgcaaac aaccgccttc atcgatgata caccctccct cgctcaagac
     9601 aaaagatgcg ctcctgagtt cgtccacgtt cttgagcata tactcaaccc cgtactgccc
     9661 gccaacttct tcgtcacagg tagcgagata tatgatgtcg cgctccggtt ttaccccatc
     9721 cttttggagc ctggcaaagg caagaagctg acaaatagct tgggatttca tatcgattgc
     9781 accacgaccg tagataaagc catcacgcac ctctccactg aaaggcggaa cttcccattc
     9841 atctgctttc gcaggaacaa catctatatg gctcaggaga attatgggct ttcctttgct
     9901 ttttccggtg attctcgcca aaaggttggc cctttttggc gctacttgaa aaacctcagt
     9961 tggtatacct tctttatcga ggcactcatc aagaaacctg acagcatctt cttcattccc
    10021 tggtgggttt gttgtatcaa tcctaataaa ctctcttagc aagtctgctg ctgaatcact
    10081 ttcatgcata agatatcctg ttctttcctc cccgtttggc agcgtaaaga ttatcatcag
    10141 caatcttgat gagtttgttt acatccattc cttgggtgaa atgggcaacg ccaccgctta
    10201 ttgttatatt gaatttccct tcatcatttg taaaagattc cttttcgatc ctttcccgaa
    10261 atctttcagc aacaatatag ccgttgatcc catcagtttc aggcagaatg gcaaggaatt
    10321 cttctccacc ataccttccc acaatatcaa ttgccctcag actgctctta agaagctctg
    10381 caagcttttg tagtaccgca tcgcccgcaa gatgaccaaa cgtatcgttt accttcttaa
    10441 aatcatctat gtcaaagagt gcaatcgaaa gaagtgaacc ataacgccta gatcgttcaa
    10501 cttcgtcttt gagagtttct acaataaaag catgattata aattccagta agcccgtcac
    10561 ggatagagag cgatctgatc tcattgaaaa ggctgatttt ttcaagagtc atggcgatgt
    10621 ttgagcaaaa tctattgaag attgctttct catcatcatg aaaagggagc accctgtaaa
    10681 gaccagcata cccgtaaatc ctgtctctgg cagagatatc aactgtcata aaattatatt
    10741 tgtcttcccc tgtactaaga gtgtcttttc cagctatagt catctcgctc atttgctttt
    10801 caacttttgt ccgctcaggt gccttattca taagagacat cgttatacat tccataagct
    10861 tctggtttga ccccacatta ggatagatct ggatgtttat atcctcaccc tggagcataa
    10921 gcgctgcaaa tgcttgaaag ttaagaaaat ttttcagaag ggcaaaggag ttctttatga
    10981 cgaaggcggg atcttcggtc tcgcctatca tgagttgaag cttttttaaa aaatcgagct
    11041 ccagaaggtt aagcctctca tgctcgtggc gcttttcaat ctctgctgta agctcaattg
    11101 ttcgctgggt ctccaaataa tggttggctg cattgttcgt gatgtggtaa acctcttcgt
    11161 aacttttaaa tggttttgcc aggattgcat aggctccgct cctaagagat tctatttttt
    11221 tctcttcatc gaggtgccct gtcatcatga taaccggcaa attggaatca atttctttga
    11281 tcttcttcag tatctctatg ccagaaatac cgggaaggaa aatatcgaga aggactgcat
    11341 tgggtttttt ttcacgaatc tccgaagcca tgtcaggaac catctcatag gtaaacgctt
    11401 ctattccgtg attacgaaaa aactctttta atataaaaag aatatcctcc tcgtcgtcta
    11461 ttacaaatgt ccgttttcga gtctccatag taaagcaatg ggggatggct ccccccattt
    11521 ttatttcttt gcttttttct tgggtgcagc ctttttggtt tctttttttg gcgcagcctt
    11581 agaggttgtt gctttcttta ctccacaagc catgtcttca cctccgcatt ttttaaaatg
    11641 catgatattt taaataaaga tctttgtcaa taacaattgc aacggctatg gacagtaaac
    11701 gttaaaagcc tggaaattta tatcaaatga ttttttgtgg tccataaccc agaacggagc
    11761 acccagaggt cttactgtac ctctatcttt acctctttcg gcactacttt ctgcgatttt
    11821 gggatggtta tcttgagaac accatctttg taagaggctt tggccttgtc ccccacaacc
    11881 tccgccggaa gctgaatgat cctttggaat gaaccataga ctctttcaga tctgtaataa
    11941 tcggcttcct gtgactcctc ttccctttta cgctcccctt ttatagtaat cctgtcttct
    12001 ataattgata tggaaattgc ctttggatcg atggctggaa tatccatcat aacaataaca
    12061 tccttttttg tttgctgcac atccatgggt ggattccagg ttccatctgc taaagacgaa
    12121 aaatccgttt taccaaaaaa atcctcaaac attttatcta tttcgtccct caaagaagga
    12181 aatctagact cccacaagcc tctccacggt gttagagcca taaccaccta cctcctttta
    12241 ctttttgttt ctcttccaga agctgaccat gccagaaggc ctaaatagga gttggacaag
    12301 ttcccatcct tcttttccca tctcgttgaa cagatccgac agatgtacca tctgttctac
    12361 acccacatcg cgtatctcac acgctccgtc aggtctgcat gcatagacaa tgtttttttc
    12421 aggatctacc acaacccctt tctctgacaa ctgttgcatg gaataggcct taatagtata
    12481 ctcaaactcc atatatttta ctccctgttt attgttaaat tattttataa tatagtaacg
    12541 catcaaccat tttttttaaa gtagcaatgt acgattctcc attatattta aaaactaaag
    12601 caataggtct cctcgataga agaattgagg aacttatgat gcatctcaat ccctgtgttt
    12661 tatgtccacg aaagtgtaaa gcaaaccggg cagcaggtga gttaggctat tgccgggcgc
    12721 catatcatct gtatgtttca tctgcttccc ctcacttcgg ggaagaatct ccccttgtgg
    12781 gaataaatgg atcaggaacc atttttcttt cccactgtaa cctcaggtgc atattctgtc
    12841 agaattacga catcagcatt tatggagaag ggtacccttg cacatacaac gatcttgcag
    12901 ctaccatggt tcaactccag gagaggggat gccataacat taactttgta acccctaccc
    12961 attacgctcc acatcttgtg agggcacttt caatagctat agacatgggg ctctccattc
    13021 ctattgttta taactgcggc ggatatgagt ccctggaagt catcaagctt cttgagggag
    13081 tcattgatat ctttatgcct gacattaagt ttcttgatcc cgccctttcc agccgttttt
    13141 gtttagcaga gaattaccca gaaatcgtga aatcagttgt caaggagatg taccgacaag
    13201 ttggcaactt atcaataaat gctgacggaa cggccacacg gggacttctc ataagacatc
    13261 ttgtcatgcc atcatgcggg gaagatacga agcgaatatt gcgatttgtg caggaagaga
    13321 tatctcagga tgctttcgtc aatattatgg ctcagtacca cccatgcaac agagccaaag
    13381 aatatcctga actttcaggg agaatctctg gaaaggagtt cagcgatgcc ctccgttatg
    13441 cccgagaatt aggccttaca cgggcaacta atcactgagc tttttgcgaa tggcatcatt
    13501 ggctttgagg acctcctggg catctttacc agtgacttcc caactgtagc cacccttcga
    13561 ttctctcttt aacttgatct taatatcacc tttcaggctt tttttaatct catccaattc
    13621 tttttgatat tggccatcca tgccctgggc ttcagaaacc ttcttttgtg atggtgcatc
    13681 cattcctttg atacatgata ttagcatgag cacgatcaca aatattgtaa aaaaacgcat
    13741 gtcgtcatac tataatagtg actgaaccat ttcaataaaa ttctggaggt gaaatgtcat
    13801 gaatctgaat gtagaagtat acggatcagg tgagccgatc attttcgtac atggtgtggg
    13861 tgggagttcg cagtactggc attatcagaa agaataccta aaagacctca tgaaggtaat
    13921 ccttattgat cttccgggcc acgggaagtc atcgggaagt gcttgcgaaa ctattgaaga
    13981 ggcaagggat atagtgcgcg aagcaatcat ggagctaggc attcataaat gttatcttgc
    14041 aggccattcc atgggcggtg gtatagcaat ttcctttgct cttgcatatc ctgagcttct
    14101 cagaggtatt ctcctcatat gcacaggggc aaaactgagg gttttacctg caattctcaa
    14161 ggaaattttg gagaataaag aagacactgt tcgcatgata atgatggaat atgctttttc
    14221 aaataaggcg cctcagaaaa tgaaagagaa cggatttaaa gatatgatga aaagttcagc
    14281 gacaactatc tataaaggct ttgcagcgtg tgacaaattc aacgccattg gttccctgga
    14341 caaaattacc ctccccacaa tgatcatcgg aggaaaagac gaccttctaa cgccaccggc
    14401 tttttccgaa tatctttaca aaaatataaa aggctctgaa cttgttatcg ttgaagacgc
    14461 aggccatatg gtgatgatcg aacaatccga taccgtgaat aaagcgataa aaaactttgt
    14521 atttgaaggg tgaggtgaaa gatgtatacc aagagcctaa tttaaaataa aatgtgggta
    14581 gctcaagatc ctggagcgac gataagagaa aataaatgaa attcagaacc gtggtaaacc
    14641 gcgaaaaaga aaaggctatc ttagaaaaaa tggaacatct tggcatccgc gaagaggata
    14701 ttgttgaacg atttattcgt tctcgcggcc acggaggaca gaacgtcaac aaagtttcaa
    14761 cctgcgttta cctgaagcat gttcctacag gcatagaagt caagtgccaa caggaacgct
    14821 ctcaggcatt gaaccgcttt cttgcgcgcc gcatactgat tcaaaagata gaggaccaga
    14881 ccctcgggag ggaaagtgaa aaacaacagc atattgcaaa gattaaacgg caaaaacgaa
    14941 agcgatcgaa acgggctcag gaaaaagtgc ttcatttgaa acaccttcag tctgagaaga
    15001 aaagggcccg ttcccacata cctgatccat gggaatatta aaaaaaacag gcaacgggca
    15061 agaaagctac agttgccatg tcaaagcagt tatttattat aaaaaagatt atgcatcaaa
    15121 ccttagaaat ttcataacaa acacattaca gcgtattttc aaaagctgac aacaactatc
    15181 tttcctgaga aactacttct taaaggcgaa tcaattttct gcgataaaaa ttacgatatc
    15241 tatggctatc cattcacact cagataccaa accactaaca aaaccaggat caatttttgc
    15301 cgatgcctat ccagattcat tactggaaat atttgcgatc ctcgtcgcac ggtttgagaa
    15361 tcgaaaaaat gaatccgatt catcagaaaa acagcccgag gaggggttgc tccctgactt
    15421 gatagctgaa gcctcgtcgc ttttggctac tcacggggga actgttattc agtcttctga
    15481 tggggcttta caggcgtatt ttcacattgg aaaaaacgct gtgaaagctg ccattgctct
    15541 gcgccatgcc cttgggatat ataatgcaag caaaaacctt cagagcccat ttcatgtaaa
    15601 aattggtgtt gattatggag aaatcatccg cgatacagac aatttacatg gtaatacagt
    15661 tgagaatgca caactcctga gcgatattgc aagccctgat gagatttgtg tctcgtggcc
    15721 ggtgtttaat ctctcaaaag atctcccagc tgtttacttt gagatggttc atccgtggaa
    15781 tagaaaaaaa attccagaag gatttgagat atatcgcatc gtctggaacc ccacccaaaa
    15841 aacaagctgc ctatcctgtg tcatcctttg ctttagacca atatggaaac tgtgggacca
    15901 aacatttgcc gacatatggg aggacctcat aagtaatggt cagactctct ggggcgggca
    15961 tagtgatcaa aaagagatac tcgatgatag gtcaattgta cttattctca agagtatgga
    16021 atcaatttta cctctgtcca tggccgttag taagtttctc agaaaaagga tccagaactc
    16081 tacgaccagc ctcattccga ttcaaatcat tgcagacatt gggccttatg gagagaactg
    16141 gcaagcacga acgaacggac ttccaccttt atgggaaaaa ttgaatccag ggtatctcta
    16201 tatctccgag caaacatatg catccttaca gcaaaaaatt gagattcccg gcaacgccat
    16261 tcatcgcaca tactctggcc agtcgtttta tcaggttggc cttgacaatg aactgccatc
    16321 acaggaaaca aagcgttttc tttaccagaa agccttggtt cagggagatc ttctaccttg
    16381 cttttactgt ggtgacagaa aacaccatcc caccaagtgt ccttcaaaaa atattcctga
    16441 aaacactggt gccctcaatc aactgggata ttgctccata gatgaactca atgacatatt
    16501 ctatcatcat atcattggag agaatggacc tggtgtaaat ccccaggtag aagattccct
    16561 tagccttcct gccagtggat tttttgaact taaaagagtt tttcagttaa ggttcttccg
    16621 atccttttgg aataccacaa acgaagaatg gaacaacatt cgtaagagca ggaacaagag
    16681 cgaagggggc cttatctggt tagcgcaaga ttcgctacgg gtatcagaat tgacaaaagc
    16741 tgaatctatg ctcgtgagcg ccatgaacaa atacccccta gattataggg tttatgttgc
    16801 ctctggtttc ctggatattg agaaaggcga tctctccagg gcagaacatg atttttctga
    16861 agcctatgcc attgcaaaaa caaatgttca gaaggcattc acccttctcc tgctttccag
    16921 actatactgg atcactggta acctggataa agcctatgag aaagtccaac ggatctactc
    16981 attaaatgta gactctgttg atgccatgta tcaagatgtg ttactcaaac tttttcaagg
    17041 gaaagaaaag ttagcgtgtc agcgtttatc aaggcttgtt caggaacaca gagagttttt
    17101 cgttgccgcc atgatcgatc cagaccttgc tccttacagc ggcattgtag gagataccct
    17161 tgaaatcatt cttaaccggg ctagacaaga tgcgcaatca gcagtatcgg atgcagataa
    17221 tgagtatgag ctttcaaagg ttgtgcttgg aaaaagtgat ctgaatgata tacaaattct
    17281 ccagacaaaa attgaacaat tattaaacaa agacagctat tgcggttatc tggacatcat
    17341 tgatcactgc aattccatca tcacaacatg cagaaaacgt acaatccata gaagaagaga
    17401 gatctgggga atattgcggg aattgaacaa acgccttgag actaatatga tgttcgttga
    17461 atcatatcca tataagagta tggtgcgttc atgccgacaa cagttggtcc atagtagtga
    17521 aaaaatccgt catgttcaga gtatcggtcc tgccctttct caagaacaac ttgcggcatg
    17581 tcacagcctt tacgaggaac tcacgaaaga atgggatagt cttgaatcaa aactgagagg
    17641 attgaattgt tttcttcaat tttgcaaagc atctctccac tttcttagat ggagcggtac
    17701 atttatggct attacctggt ttttaggcct tttccttttt ccgttgatca tctactacct
    17761 aaatgctttt ctgtcagggt ttgctacctt aacgttttct aatgtctggt tctaccagaa
    17821 aaattgtgta ctttttggaa gccttgcagg aatcggtgtt gcactggcaa taacgataaa
    17881 aagcctcttc aaaaagtaaa aagctcgcca aaggcatggg tgcagccttt gcctatcgtc
    17941 ttttgcctct tgtttgcttc atctcaaacc tgagacactt agtacccgat gttttatgaa
    18001 ccagggtgga gggcataccc gcactcttaa aacccattgc tttgcatcct tttggataat
    18061 acttatccca tgttatatag aaatgaatac agtctacaca tttaacctgt tgtttgtcct
    18121 cttctttagg cataacaaca atatagcata cccgattttt aattttcatc catccttcaa
    18181 catacatgat gtaaggtgtt tacaattctg ttataataga atcaaagaat aaaaaaacgg
    18241 tgaataacat ggatttaatg gccctcgcag aactactcct tgtttcgttg tatgacctct
    18301 cagaagaaca gccacatagc tacttctttt ttcagcttga ccagattgga acccgggtcg
    18361 gtgcaacaga catggaacaa ttgaccaagg cagcccacat tcttgaagac cgggggtttg
    18421 ccatgctttc tgcaagtcct ctgtctatct tgagtgccat gattagcagt gatgggatat
    18481 cctttgtgga aagcggaggt gagaccggca taatcaagac atatcgcaac aatccccgtg
    18541 aggttatcga tattgtttgc aaagaagtgc aagcaccacc agaatctgtc tcgaattcta
    18601 ttcaagaaga tatacctgca accgtacaag ccttatcttt gaatgagact atgcagacaa
    18661 tcctttcccg cataggttct gttctggctg atgattcatc aataataacc actgtgcgtg
    18721 aggatgcaat aaaggacctt gagagcttaa agattcagtt gtcgaggagc tctcagaatt
    18781 ggcgacttgt agagatgctt ttggatgccc ttgctgaaat cccatctctc ctgccaagtc
    18841 tcaaagacct tgcacttgta ctgagaagat tagagtgata aggtcagcgc catctgtagg
    18901 caaatgaaaa aataaggcac tggaggttac catggcgttt aagctggatc atataggctt
    18961 agttgtagag aacatagata agatgaagga attgtttgga acaataggtc tggatacagg
    19021 tacagaagct atagtcaacc ctttgcaaaa ggtttctgca tcttttgtga atgttggcga
    19081 aaaagacgac atatatattg aaatccttga acccgcttcg aatgattcgc ctattagtaa
    19141 atttctccag aataggggtg gcggtttaca ccatctttgc ttcgaagttg acaatattga
    19201 agatgcaatc aaagagctca actcaaaggg attccggatg gtagtaccac cggaagactg
    19261 cagggcatat gatgaaaacc tcaagagaag atgccaagaa ccaacaaaaa tagctttttt
    19321 tattgttacc aaaggggttc tcatagagtt aatagaaaaa ggcgggtagc cgtaagattg
    19381 caaaaccaca aacaaatcgt ttatatatga aatgatgtac cgtttacagt aaagcctatc
    19441 aaagatacct agatgattct catcacgaaa ctggagaggt aaagaaataa tcaatagtaa
    19501 caaacgccga aggtcgaagg tcgaaggttc aaaacaacta tcttatatca gcatctttac
    19561 atctcggcgc atggcagcca tgcttctgct gggatttgcc tccggattac ctctccccct
    19621 gacaggggga acccttcagg cttggctcac gattgcaggg gtcgatctta ctacgattgg
    19681 gatcttttcc cttgttgggg tgccgtatat tatcaaattt atgtgggcgc cactcatgga
    19741 tagatttgtg ccaacgtggt tgggaaggcg ccgcggctgg attttcccca tccaacttgg
    19801 cttatttttc ggtatcggcc ttatgggatt cacacaacca tcttgcgcac ctctaatgct
    19861 cggtgttctt gcattcctgg ttgcctttac ctccgcttct caagatatcg tcatcgatgc
    19921 ttatagaaca gatattcttt acgaaaagga acggggtatc ggagctgcaa ccttcgtact
    19981 agggtaccgt gttgccatgc tcgtggctgg cgctcttgcc ctcatcattt ctgatcacat
    20041 agggtggcac aagacctatc tgattatggc cctggtcatg attgcaggaa tggtcggtac
    20101 ctttaccggg cctgaaccca aagagaatac tgcttctcca aaaagcctga aagaggctgt
    20161 gataggacca ctccaggatt tcatggctca cccatacgct ttcagcatga ttcttctaat
    20221 tgtcttgtac aaactcgggg atgcttatgc cggaaccatg acaacaacgt ttttactgcg
    20281 gggcgttggt ttctctgcaa cagaagtcgg aaccataaat aaagggatgg gccttgtggc
    20341 aactattgta ggagccatgt tcggtggaac gctcatggta aagctgggcc tttttcgatc
    20401 cctcatgatg tttggttttt tccagatggt ctcaaatctt tctttcatgg ccctggcatg
    20461 gtttgggaaa agctacgaac tgatgatctt tgccgtagct tttgaaaacc tctgcggtgg
    20521 gatgggtact tccgcttttg tggccctcct catggcgctg tgcaacaaaa gatatagtgc
    20581 gacccaattc gcgcttcttt cgtcattgtc tgcatttggt cgtgttttga tatctccaag
    20641 ctcaggatat gttatcaata taaccggttg ggccatgttc ttttttatta caactctggc
    20701 tgcccttcca ggcctctggc tcctttggag gctgagaaac cagattgaca atctaaagca
    20761 ggggcagtca attgataact catagctgta acctcaaaat aacaacgatg tcttacgctt
    20821 cttgcttcat tccttcctgc tcgttgtctg gactcttctt ggcctcaaaa cgaggaagcc
    20881 gggcttatcc accatgtatc cttcaaattc ctgatcgatc accttccctt gcactgggga
    20941 ggcatgccgg aaatggggtg tgcctttttt tctgacaaat atccccacat gagtaacatc
    21001 gaggccaggg ttatcagcat atattccgat atagtcacct gttcgacagt ttttcagaac
    21061 aatgttttca atagatttgg cagggatata atctattgta cgctcaaccg gttttatgca
    21121 agggacatag aaggctccat tctctttcag gttaagtatt ttcttggaaa acatcgtata
    21181 acgaccgcct accttttttg taacttcttc aatgtatgtt ctgtttgcaa caatccagtc
    21241 ggtaaaaaaa tgattgcgat gatcaaatgt aacgatgcca gaacgatatc gagtgcaaat
    21301 aagactctcc tgaaaggata aaaaagaatc agcgaagcag agcgcctcta cataatcaat
    21361 aaaggtgaag cagtcaaccc ctgagagatc cacaacaaac ctctctggtg ttttatcatc
    21421 gccaataagc gtcttccctc tataactcac tccaaggaac tgtcgtgaaa tatattcaac
    21481 ccttcgtcct gtcgattgga tttgagcggc cttggctatc atctcctcaa actcgtcgag
    21541 tgtccaacgg ccgaggttta tactggaact gatatttctg cccatagtgc aaatgtagta
    21601 taagctcacg atttttacaa tggaaacctt gtaccaagat catgaaattt ctgttatgct
    21661 catagcatga gaatagttga tgtgcacacc cacggcctgg ccggatacag tacgaacaca
    21721 atgaatcctg atgaaatcct ggaagtagct cgcttgcacg gtatgcatgg cgtaacagat
    21781 atcattctaa caatctatcc cgggcctgtc gagataatga aggaacacat gactgctgtg
    21841 gaggaagcaa tcaagaggca gcgattagca ataagcagtc agggatcagt aagagctgaa
    21901 tcagaacacg aaactcaaaa tccaaaactt gcgagaatac tggggattca tcttgaggga
    21961 ccgttcctaa acccaagcaa ggcaggggca ctcgatgcat cctcctttct aaaaccgtct
    22021 gaaaaaatat gggaaagtct gattgagggc tttgaaggta ttgtgcgaat cgttaccatt
    22081 gcaccggaac ttgagggggc aaaaactctt attaaagcca tctcgaataa aggaataatt
    22141 gtatccctgg ggcattctga ggccacatat gaagagacag aaaacgcgtt tcacgctgga
    22201 gccaaaggca ttacacacct ctttaatgcc atgaggcttt ttcatcacag agagcctggc
    22261 attgcaggtt ttggtctcat gaacccccat atctatgtcg aggtgattgc tgatccttct
    22321 cacctcaacc gtcgaaccat tgagtttatt tttaatgtaa aaaaccatga aaaaattgtg
    22381 atcatttccg actcaataaa ggccacaaaa gttggggcag gtcaacatgc tcttatgaac
    22441 agtgcaagaa ttctcgaggg tggaagcatg accgtcacag aatcagcgtc aaggcttata
    22501 gatatgggct ttgatgaggc aaaaattata ggatgtataa gtacgaaccc cttgtcctac
    22561 ctcgcactca ctaagcagtg ctaaaaaggg atgtctatca tctaaaccgt tttgattgct
    22621 ggaacagtct gaacaaacaa gtttattgtt ctgctatata caggaaatgc tgatccttca
    22681 aagaaaatga aaaaacagaa aaaccctaaa accatgaaaa ccagaattaa caacttccgt
    22741 gaccaatcca ggttcaggat aaattgaaaa aaaattaatg cagcaaaaac aagacagtat
    22801 gaacagtaag tgctgttcat aacctgatat ccaataagaa agacctcaac acctatacca
    22861 gctgaaagca gtacgaggac aatgaggcca tttttaagaa tattaaaaaa gattaatata
    22921 accacgtata ctatgcccag gtattgaagt tcgattccga aaatggtccc ctggaggtat
    22981 gcgcatgaat ccccgcagag ggtatagaaa aaaacaatgc ctgctcctat gatcagaagg
    23041 atgatgttcg ccgcgaatct tttattgaga agccatttca tgggattgtg ttcagctgta
    23101 atacgagatg attatacatc aatttaagta ataaatgcca cgtttatact ttttttctat
    23161 ctctttgaac gttccaccaa tatcatgata tcctacattt gaaaacatcg agatgggaat
    23221 ttaagattat ggcaaattta tgaaatagat ggggaaacga taaaaaaagg atgaaagcct
    23281 gtatcataga cacaacatta tccttttttg ttgtgcccgt gtgcaatact ttaaaacaga
    23341 caacacacat taaagacagt aaagctatta attgagaaga agtgcggttc atgccatggt
    23401 attaaaaaaa ctacaggcca gaaaagagac cgggctggtt ggctgataca atgagtgatg
    23461 cgcatgaaga agcaggaatg gtgtgtttgt taaggacaac gaagcagggg taatcatcga
    23521 ttatctggca aaaaaattac gagaaatggg catgggttac aggcaacgaa aaaaaccgct
    23581 catagctgag cgcttctggc ctgccttgag aagcaaaaat caaaatagta tagattttca
    23641 tctatactcc attgccctcc gccagttatc ctgggagcag tagctttata aaccctacaa
    23701 ttatcattct gaaaaaagca ataagggggt tgagtgcccc aagccacagt aatccaataa
    23761 tgatgaaaaa accgtatggt tcaagcctgg caagagaata ttgaaagctt ctcgatgaaa
    23821 aacccatgag aatcttggaa ccgtcaaggg gagggatagg tatcaggtta aaggccgcga
    23881 gcatgatgtt aatttgagcc agatagaaaa gaagtgtgtt taaagattca atatgccatc
    23941 gagacaagag ctgaagaaga aaaagggcaa taaacgcaag tataatgttt gcagtaacac
    24001 ctgcagcaga aacaaagata aggccttttc tgaaatccct taacctgttg aaatttaccg
    24061 gaactggctt tgcccatcca aaaccaaaga taaacaacat tatagttccc atagggtcga
    24121 ggtgcttgac agggtttagg cttaaccttc ccaaccatcg cgctgttgag tcacccatct
    24181 tgtctgctac ccaaccatgt gccagttcgt gaataattat cgagtagagc aggggaatgg
    24241 ccacaaggac aaatgcaaca gggtccttaa ataatagatt gataagcccc atatcgtcac
    24301 ctaagtatca cataggcatc agctgttttc aaccaaattt gatttatgtt attgtgttta
    24361 cctcattcaa ctcacgaatt cgctttgcta tgatctttaa gctttcagct cttccttccc
    24421 tttccgattg cccattggct ataactaata acctatcttg cgctaccttc tctccttgac
    24481 ttcaaccgtc ttttaccata atatatgcaa aatttttcga gaggtgatca tgaaaaaagg
    24541 tatcgttctg ttgttcactt tgacggtttt tcttttcggg atattttcga ttactggtct
    24601 tgtcttcgct gacaaacagc cacctgattc aatagtaatt aaaaatgagg gcgcgaagct
    24661 tccgcctgtc aatttctcgc ataagtccca tgtggaaaaa aacaaactgg aatgtgtgaa
    24721 gtgtcatcac aaagacacta atccaaaaga accggagcgt tgcgtcaagt gtcaccttat
    24781 ttcagaggtg aaggacaatg cacccatcgc taaagatgca tatcacaaac agtgccagac
    24841 ctgccacaag gacagctcag caaaaggagt gaaagcgccc acagcctgta atgaatgcca
    24901 taaaaaataa ggctgctgag caggtaaccc ctccaggaaa aagtttattt caatctttac
    24961 agggttttat tgcttcaata aaattttcaa taatcctgct tacctgtatt gctcttggat
    25021 caattattgg aaccattatc aaacaaggtg tgggaccgga tgaatatctt tccctttatt
    25081 ccgagcatac ataccaaatt ataaacctgc ttggtctgaa cgatgtctat cattccccgt
    25141 ggtttattgc cctactcttc ctctttgcta ttaatctgac tctttgcacg gtgaggcggt
    25201 ttatgcccgc atttaaagga agtaaaaagg cctctgttcc tgaagaaagt gcaatgatgg
    25261 acatgtcaat gagtttccgg gcaagcacaa gtattgatga tgcacacctt ttcagcatat
    25321 tgaaggggta tcggatctca tattcaggag aagaaggcaa ggtttttcaa aaaggtgcac
    25381 tttcaaaata tggagtatta cttatccaca caagcatcat gcttatcctc attggcggtt
    25441 ttgtagggtt ggtagcggga tttaagggtt ttatggtttt acataaggga gacaccaaaa
    25501 atacgatcac tattggtggt gaaaagcctt atgaaaagcc tctgggtttt tctataaaat
    25561 gcaaggaatt ccaggttagt ttctatccga atggagcacc aaaggattac gtgagcactg
    25621 ttgaggtaat agagaacggc aagattatct tcgaaaaaca gatacgggtc aatgaacctt
    25681 taagctacaa ggggattcat atataccagg caagttatgg aagcgcccca tccttcactt
    25741 tcacaatcgg tggtgagcgg gtttccttaa gagagaagga ggcatatcgt aacgataaca
    25801 tctccttgat gcccatccgg ttcgaacagg atatacacaa tttcggcccc ggggtcatgg
    25861 tggcctatct cgaacagggg gagccccgca cagtctggtt tgtaaaaaat attgagcgct
    25921 ttaatgaaaa aattatacaa ggcgtgcccg tacgtttcgt agatattcaa gagaatatct
    25981 atactggtct ttcagtgtca agggatccag gcatatggat tgtctgggca ggattcgctt
    26041 tcattctttt tgggctctat ataaattttt ttcagtatta cagacggatt tacattcgaa
    26101 atattgcagg cgggtatctt gtggcaggga tcgccataaa aaataaagat gcgtttaaaa
    26161 aggaattcga gatgttgagg gaaaagctaa atggcattcc atcataaaat attgggcatt
    26221 gttacactcc tctatttgtg tctttttttt atacacctca tcttttttgc ctttaaaaag
    26281 gaaaaaatcg tggccataat gtggcctctt ctttatggga cgctgggtct tcacacggtg
    26341 ggactcattc ttcgctggct ggaatcatat cgccatggca tgggacatgc ccctttgtca
    26401 aacttctatg agtctcttgt cttttatgcc tggtgtgtga gtctgattgt tgtgtttatg
    26461 aaaaagcgac tgaactaccc tgctataact atatgcgcta cggtcatttc actcttcctt
    26521 atgggttatg catcgatttc tccatccgtt gacagaaata tccaacctct cgtacctgcc
    26581 ctccaaagca actggctcca catccatgta cttacgtgtt tccttgccta cgcctcgttt
    26641 gccgtctcat ttattagtgg gtttctttat atgtttacct ggaaaaatgt tattcctcca
    26701 cgggatattc ttgatgaaat taattataga agtgttgttg taggcttccc catgttaagt
    26761 gctggtattt ttacgggtgc agtctgggct cattatgcat ggggttcata ttggagttgg
    26821 gatcccaaag aaacttggtc actcataacg tggattgtct atgcggtttt tcttcatgca
    26881 cgcctcatga ggggctggaa agggaaaaag attgcaattc tttcaattgt tggttttgca
    26941 agtgtcattt ttacttattt tggtgtaaac tttatacttt caggattaca cagttacgct
    27001 acgtgaattg caaaaaatac tgttggaagg tggaaaaaga aacctggaag ccatgaaaaa
    27061 acgaaaaccc aaaagcctta ttttagtcgg aaatggcatt aataccgagt atgagacggc
    27121 ccatgcccat agactcgttg gattcgaacc cgattttatc catataaata ccctcattga
    27181 ggaccctcac aggataaagg ggtacacatt catcacttta cccggaggtt ttcttgatgg
    27241 tgatgacctg ggctctgcaa aagctcaggc agtgcgctgg aaataccgca aaataaataa
    27301 aacaggaaag acatttattg atcttctcat ttcgtttgtt caagaaggaa atatcatcct
    27361 cggtatatgt aacggttttc agctactcgt taaaacaggc ttgcttcctg gcctcggcag
    27421 tgagcattct acccagaggg tgactctcac atttaatgac ctcggcagat ttgaggatag
    27481 atgggtttat ttgaaaaaca atacattttc tcattgcatt tttaccaaag atatccatac
    27541 aatctatctt ccagtgagac acggagaggg taagtttgtg gtggacacag agaaaacgct
    27601 tgaaagaata aagcagggcg gacatattgt catgcagtat gctgatgact caggaaatgt
    27661 tacctctcaa taccctttta acccaaacgg ttctattgag tcaatagcag ggctatgcga
    27721 cagtacagga agaattttcg gactcatgcc tcatcctgaa gcatttgtcc atcggattca
    27781 gcatccaaga tggactcgtg aagcgttacc agaagaaggg gagggcttaa tgatattcaa
    27841 aaatgctttc aaatatgtga cagaaaaccg ataggaggaa tatttcatga ataagatgga
    27901 tgttatcaat aagctcggtg aagatctcag gctgaaccaa aaaattgcaa agatagctgt
    27961 tgatacaata attgatacca taaaaaatgc catcatcaat ggagaaaggg ttgaaattcg
    28021 tggctttggc agcttctcac tcagaaatta caagtcctat accgggagaa atccaaaaag
    28081 cggagaggta gtccacgttg aaccgaaaag acttccctat ttcaaggttg gcaaagaatt
    28141 gaaagatatg atatggaagc cgtaaaggac agaatataag aattaagaag ttgagaaaag
    28201 actgcaaatg gtttgatggc tcacttctca acttcttaat tcttatatgc cagtgaatta
    28261 atctattaca gttctcccca aaaaggtctt aaaacctgtt ttactttgtc gactgtttct
    28321 tgaatttcat ttgatgggtt tatcttctta tctggtgtaa acatggatgt tcctttaagt
    28381 tccatcgtaa cggctttaac agatgtagcc aaaccgttca aatcatatcc accttcaaga
    28441 gcacaaatca ccttgccatt gcaatgcttg tcagcaatct ccaaaaggat ttttgtcatt
    28501 gttgcaaagc caccctccgt tactgacatt ccacccagag gatcattata gtatgaatca
    28561 aatcctgcag aaacaagcac cgcctcaggt ttgtattggt ctgttattgg gattaaaacc
    28621 tccctgaaca cgtactcata atcatcatca cccatgccat gcgacaaggg gatgttgata
    28681 gtatagcctt tgccattccc ttctcctgtc tcattatacc agccggttcc gggataatag
    28741 ggatactggt gtgtggaaaa gtaaaggatt gatgagtctc cataaaaact gtgttgggtg
    28801 ccgttgccat gatgcaaatc aaaatctacg atgacaatcc ttttcaaccc atatcttttt
    28861 tccaaatatc tggcgccgat ggcaatgttg ttgaaaagac agaatcccat cgccctgttc
    28921 ctctctgcat gatgtcccgg aggtcttacc aaggcaaaac cattctgtat cttagaagaa
    28981 aacagcatat cagcaagctg cagcacgcca ccagcagcca agcatgcagc ttcataggac
    29041 ttgggtgacg taactgtgtc tgggtcgagt cttctttgca tcttaccctc ggttgctgca
    29101 attgaggcta tatacgcatg atcatgaatc agagcgattt catcgtgtgt cgcaaccctt
    29161 ggaggcgtgt atgtgatgcc ttctttatca atgctattta acatcgtgta aatatatttc
    29221 aagcgattag ggttttccgg gtgatagtcg tctgttatgt gttccagaaa aatctcgtcc
    29281 cttactattc caacattcat ggcaccctcc tttgcgacaa tgatagtacc ttttctgtta
    29341 aaaatcaaga aaagtatagg caggaggcaa tatgcaaggt actgcaaaat aggcaaggca
    29401 gtatgagaga caaagattgg tattgtaaaa aaacagcaaa gcatttaaaa aaacatctta
    29461 tccgacgata ataatgaaag accttctgga aggtgagaaa tgtttaaatt atgcaaaacg
    29521 cgcacaggtt gcggtgtaca taaagaaagg gattgccttc agaatagact actttgtaaa
    29581 aggcttgcaa atggtattat tccaagataa ctgtgttttt aagtatataa cgattaaaaa
    29641 ttcaatggtg atatcactat gcctctccgt ttaggtgacc tcctcgtaaa aatgtcgcgt
    29701 atcactgaac agcagctcat tgaagcgctg aaagtccaga aaaacgaagg cggtaagctc
    29761 ggagaaatca ttataaaaat gaatttcatt actgaaaagg gcatccttga ggccctttcg
    29821 gaccagtttg gcataccaca gattaacctt gaccagacag agattaaccc tgatgtaatt
    29881 cctcttattc cgctcaacac aattcgcagg tttcacataa tgccgatacg caaaatgaat
    29941 aacatgctta ccattgctat atcggatcct acaaaagctt ttgatctcaa agagattaag
    30001 cttcttaccg gcctggacat tgagcccctc cttgcgtctg aaaacgctat ttcaaaggca
    30061 attgaaaccc attacgagac aaatcacgct gcagaactga gaaaatttat ggacggattg
    30121 ggggtggggg ctgatgcatc cctggagatt gttgaagaag aagaaaaagg agatatcacc
    30181 aaacttgagt ctgatgccaa gcttcctccc gttgttcaga tagtaaatca tatttttact
    30241 gaagccattg caaaaggcgc aagtgatatt catatcgaac cttacgaaga cagagccaga
    30301 gtccgttatc gtattgatgg actgctttat gaagctctaa cattgcccct taaatacaaa
    30361 gatggtgtaa tatcaagggt taaagtgctc actaagatgg atatcgctga gaaacgtctt
    30421 ccccaggacg gcaggataaa gatccagatg aagctttcag gaaggatgaa aaacctagat
    30481 atccgtgtct ccaccacgcc gacaatcttt ggggaaaaag tcgttatgcg tcttctcgat
    30541 agagaaggtt taatgctcga tatggcaaaa cttggatttg aaccggagag cctcagaaaa
    30601 tttgaagagg ctatctttaa gccatgggga atgatcctcg taactggtcc cacaggatcc
    30661 ggaaagacca acacccttta cagcgccatc ggtcgaataa actcgcccga tacaaacatt
    30721 atgactgttg aagatccagt ggaattcaac cttggtggga tcaatcaggt acaggttcat
    30781 gaatccattg acctcacctt tgcttcagtt ctcaggtcat ttttacgtca ggaccctaac
    30841 attattcttg ttggtgaaat ccgtgacaat gaaactgctg atattgcagt gaaagcatca
    30901 ttaacaggcc atcttgtatt gtctacactt catacaaatg atgcaccttc gacaattatt
    30961 cgtctaatag atatgggtat cgattcttat ctcgttgcca cctctgtaat tcttattgct
    31021 gctcaacgac ttgttcgccg catctgctct gcttgcaaag aagagataaa cattccaccc
    31081 aaagcccttc ttaacatagg cttctctgag gaagatgcgg caactgtgaa gatttttaag
    31141 ggaaagggat gcccaaaatg caacaataca ggttataaag gccgtgttgg actttttgag
    31201 gttatgcagg tttcccccgc agttcgtgac atgatccttt cagacgcaag tgttgcagac
    31261 atcaggaaac agtgcatcga tgaaggaatg cttacccttc gccagagcgg ccttgccaag
    31321 atccgcaacg gtgttaccac aatcgaagaa gtcatcagag aaacgttctg agaagcccat
    31381 agctgagagc taaaatcgaa aaacaatccc ctgttaaaac ctattgcccc gaggcctctt
    31441 gtctatagcc agcaacttat gttgttgcta atttctcgtt gagaatatct gtaatgaaca
    31501 tatgaccggg tgcgtgggta atacatatgt taggcctggc ttccattgcc gcagcttgag
    31561 gggtcacgcc acaagcccag aagacaggga tctctccttc ctttattgtt accggatcgc
    31621 caaaatccgg tttttcgaga tttttaattc caatgatctc tggatcgcct atatgcactg
    31681 gtccaccatg gacaaaggca tatcgtgatg ttatctgaac caccttgaca acttccgctg
    31741 gattaacagg cctcatggtt acgaccattg gtccgtgaaa aatcccggca ggtctgcatt
    31801 taatgctggt cagatacata ggtacgtttt tcccttcctc aatgtgccgg attggtatac
    31861 ctgacctgat aatggcctct tcaaaggaaa agctgcatcc gagaaaaaaa gtcacaaaat
    31921 cttttctcca caattctttc acgctggttg gggatgcttt caggtctccc ttcacgtaca
    31981 cattataccg tggagcatcc gttctcacat ccgcctcaga ggccagaaaa tcgggtcgga
    32041 aatcgccggg ttcaaggact tcaaggagag ggcatggctt tgggtttctc tggcaaaaaa
    32101 gaaaaaaatc aagggcatac ttttttggaa ggatgacaag gtttgcctgg gcatacccca
    32161 aggcaatccc ggttgtcggt ttatcccatt tgccagagcg gataaccgcc ctgatcttgt
    32221 caggcttcaa tgacgtgatg tctttaatca acaccattgc agataccttc cggtttcctt
    32281 atttaccgga cttttttaaa gtccgagaaa tgccttttta atagactcgg tgttgagaag
    32341 ctccttgccg gtaccatcaa ggatcgttct gcctgtctga agaacatatc cccgatctgc
    32401 aatctcaagg gactcaaaga cattctgttc cacgaggagg attgtcatgc caatagtcct
    32461 gagcttggca acagcctcta gcatctcgtc tacaagctta ggcataagcc ccaaagacgg
    32521 ttcatcgagc atgaggagtt tcgggttgga cataagaccc cttccaatag ccagcatctg
    32581 ctgctcgcca ccactcaatg tcccgctagc ctgctgtctc cgttctttca gtcgcggaaa
    32641 aaggtcatag acaaagtcaa ggttcctgct tataacagca tgatccttta tgatatgtgc
    32701 accaagcttg aggttttcct caactgacaa cggtccaaaa ataaggcgtg cttctgggat
    32761 atatgtgatt ccgagcctca ctatatcagg cgtggcaagg cggtttatca aaacatcatc
    32821 aaaagtgatg gagccttttg ttggatgaag ggcacctgct atagttttta acaatgtact
    32881 cttgccagca ccattcgagc ctactaccgc cacaagctca ccttgtttta cctccaggga
    32941 cacatcatgg attatgggaa ttccaccata acgtacctca accttaacga cctttagcat
    33001 acttctcacc aagatacgcc tttatcacat tatcatcctt cacaatgtcg ccaggcacgc
    33061 cttcggcaat ctttataccg ccgtccaaga caataaccct ttgagaaact gtcataacca
    33121 tctccataat gtgctcaatg agaaaaaggg tgatcttttt ttctacattg agtcgtttca
    33181 gaagggctac catttcgtct gtttcagtat ggttgagacc ggaggcaacc tcatcgagca
    33241 tgagcagctg aggttttgta gccaaagccc gcgccatacc gacctttctc tgctttgcaa
    33301 tgggaagttc attaatgtac tgttcgctta tctcttccat gccaagaaaa tgaagcattt
    33361 catgggcctc ttctctcgcc tgatgccggc tgttagtcct gcagaaagct ccaaccatga
    33421 tgttttcaac aaccgtcatg cccgttgtca ccttcataat ctggaaagtt ctaccaatac
    33481 cggcctgatt ggtctgaaaa ggcttgaatc ctgtaatgtc atttccttta aacatgacct
    33541 taccataatc cggactataa tatccgacaa tacagttgaa gagcgttgtc tttccagcgc
    33601 cattaggccc aataagcccg acaatctcac cttcctcaat ttgaaaagag acattgctgt
    33661 ttgcaacgag tcctccaaaa tccttcacaa tatcacgaac ttcaagaaaa gccacgttat
    33721 tttcccttcc ctctgagttg ttctacaatc ccccatatgc cgcggggctg atatgctgac
    33781 acaaccatg
//
LOCUS       DAOOMC010000011        33083 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdus sp. isolate AMR_MDS_2685
            NODE_1110_length_33083_cov_6.529975, whole genome shotgun sequence.
ACCESSION   DAOOMC010000011 DAOOMC010000000
VERSION     DAOOMC010000011.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864760
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdus sp. (wastewater metagenome)
  ORGANISM  Syntrophorhabdus sp.
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae; Syntrophorhabdus.
REFERENCE   1  (bases 1 to 33083)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 33083)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,729
            Genes (coding)                    :: 2,708
            CDSs (with protein)               :: 2,708
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 7 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33083
                     /organism="Syntrophorhabdus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1110_length_33083_cov_6.529975"
                     /isolate="AMR_MDS_2685"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2699756"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1222
                     /locus_tag="PK125_02005"
     CDS             <1..1222
                     /locus_tag="PK125_02005"
                     /inference="COORDINATES: protein motif:HMM:NF012239.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HPB36914.1"
                     /translation="EMEEKALPIVITGHIDHGKSTLIGRLLYDTGILKEDRYQEITAS
                     SSLLGKETEFAFVLDALEEEREKGITIDTTQIYFQTPKRKYVIIDAPGHKEFLRNMIT
                     GASYADAAVLIIDSNEGIMEQTKRHGYLLSMVGIKDICVLINKMDLVGYSKDIYDRVV
                     AGMKDFFGNLSLRPRFYIPISALKGNNVAKRDPSITWYEGPCLFEALDELEFKTFEER
                     PFRFPVQDVYRIGNEKIVAGRIESGEVAVGETVYLLSKGLRVKIKEIRKFLEDNIKTA
                     SYGESIGLIVEGANGIKRGDILSGDNNVRTVKDFDANLFWFYGEYNKGDKIQIKCTTQ
                     ESYCSLSITEKFDPATMDKKKEGLHHLEIGEIAKAKIKLRKKIAIDTFSYIPEMGRFI
                     LEKNGIPVGGGIIV"
     gene            complement(1256..1624)
                     /locus_tag="PK125_02010"
     CDS             complement(1256..1624)
                     /locus_tag="PK125_02010"
                     /inference="COORDINATES: protein motif:HMM:NF021575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2304 domain-containing protein"
                     /protein_id="HPB36915.1"
                     /translation="MSFRIKIVTFVICIAMLLLVVDLVRRRKLREEYSQLWFATWFGI
                     LVLMVWFDLLKWISHFIGAVSNVSTLFLFAFMFLILISLHFSVVISKLTNRLKDLSQQ
                     YAILRNELESMKKARTDTAQ"
     gene            complement(1651..2382)
                     /locus_tag="PK125_02015"
     CDS             complement(1651..2382)
                     /locus_tag="PK125_02015"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HPB36916.1"
                     /translation="MVENELERTIVIIPAHNEGAHIAPIVTQIKANYPYFDVLVIDDG
                     SSDDTRARALDAGASVISNPFSLGYGGALQTGYIYATRNRYNFLVQLDGDGQHDPTCI
                     YDLINPVQSGTADITIGSRFLISQKEGLDWPAYRAPVVRMIGMRLFSAIATFFTGQKV
                     TDPTSGYQAMNRRAFHLLAGDKFPIDYPDADVIIMLHRAGFRIKEVPVRMFPSPDDKS
                     MHSGLKPLYYIFKMFLSIIVTLLRR"
     gene            complement(2369..2908)
                     /locus_tag="PK125_02020"
     CDS             complement(2369..2908)
                     /locus_tag="PK125_02020"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012360.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HPB36917.1"
                     /translation="MMEQYKKANRLFFSFGDLYKAIMGLIAYHVPYPSFITALLHKMR
                     GVRIKNVWSVFIAYHVVIDSIHPDMVEIEEGAWLTREVKILAHYNPTPLQRDIVGGKK
                     IENVKIGKGAFVGVGAIILPGVTIGEGALIGPGSVVTKDIEDYMWVAGNPAQPIKSLK
                     ELKAGPDNNRSVVRSDGRK"
     gene            complement(2919..4589)
                     /locus_tag="PK125_02025"
     CDS             complement(2919..4589)
                     /locus_tag="PK125_02025"
                     /inference="COORDINATES: protein motif:HMM:NF013799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HPB36918.1"
                     /translation="MALNQKVFVLGLDGASPEIIESLIDRGRLPSFKALKDGGASGKL
                     RTTIPPITGSAWSSFMTGKNPGKHGIFDFIYRKEGSYELSPISARMRNGKAFWTIATE
                     ADRKVCIFNVPVTYPPDPVNGIMVSGMLTPSGRNDFTHPFSIAKELDRVAGKYQIHIT
                     ESYSRKQEDRFLKHLYEVTSKQKQAMDFLLSQEDWDLFVAVYQGIDVLQHELWHTYDR
                     RHFRHGQYEERYIDVIPQFYEYMDSILCDVMDWCERENAILVVMSDHGAGALKKLFYV
                     NNFLIKKGFLKLRKRVMTRFKHLLFRLGVSPMTFYHILLSVGLGRLKHKTRFGQGSSW
                     LKKFFLSFEDVDWGTSKAYAIGSTAGQIYVNLEGREPGGIIRRGEEYKAVREEIIKEL
                     RGLTDPETGHHVIEEIYRKEELYSGPHTLTAPDIVFLPKGLEIVAFGEYEFASHRTLD
                     YSRGLSSSHRMDGILMVKGAGLKSNSTIINGNIMDIAPTILYLMGLPVDPDMDGMVLK
                     DAIEKEILEQNPIETGDLNTCSFATGEVYTKEEEEELKAHLKSLGYFE"
     gene            complement(4643..5641)
                     /locus_tag="PK125_02030"
     CDS             complement(4643..5641)
                     /locus_tag="PK125_02030"
                     /inference="COORDINATES: protein motif:HMM:NF015651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HPB36919.1"
                     /translation="MNRVVWQWLLALFLLVIGVYYWGLSDVIKISVVSRPAIGLVFFF
                     TACFTFIHNIRWLKVLKSLLSELPNKRLDFLHFYQWLMNSYAVGLFVPSDVSLVGVRI
                     FFMKQYKEVKTSTALLSVFLDRLFDVVVLLVIIISTFLMLLGKNKLGMLIIPVGIVGM
                     VFGLFLWKNNEILPYLIVLYSRAINLVLRLPILKRWKRTDPAAVLEYIPIKKNTLCSV
                     LAWSFIKYIFIALRFYAVGVVFGITFTFFDVFFAASLVQLAGFINVTPGGLGVIEMGS
                     YGALKFIGASHPEAVAFAVGQRILVSLIIVAITLAINLAMFARSGILKSGLFAESR"
     gene            5828..7279
                     /locus_tag="PK125_02035"
     CDS             5828..7279
                     /locus_tag="PK125_02035"
                     /inference="COORDINATES: protein motif:HMM:NF015049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family protein"
                     /protein_id="HPB36920.1"
                     /translation="MLFNSYVFILLFLPLTLSIYFFLSRKKLILASKAWLVLASLFFY
                     GWWNPVYIPLILGSILFNYGIGLTLTRAKQSQSRLFLIIGILGNLILLAYFKYTDFFI
                     NNINHITGSHIRLLQIALPLGISFFTFTQIAYLVDVYRRMAKEYSVLNYFLFVTFFPH
                     LLAGPIIHHKEMMPQFDRLKNKLLNNKNLVHGLLLFFVGLFKKVIIADTLAVWVDQGF
                     DHAVILNIVEAWTVSLSYSLQLYFDFSGYTDMALGSSLMFNIRLPINFNTPYRSINIQ
                     EFWRRWHITLGRFIRDYVYIPLGGNRVSEIRIIINLMITFFIIGLWHGAGWTFVCWGL
                     MHGLAIVVQRTWKRLNIHLPKPLAWLLTFNFVNFSWIFFRARNFDDAIKVVKGMFGAH
                     GLGVTGYINHAFESFKTFRAALKYWLENIDGNIYTLVFIIICLMLSIYFRNSNDMEQR
                     FSPTWKNACMLGLLSLYAMLNLQKASQFIYFIF"
     gene            7354..8475
                     /locus_tag="PK125_02040"
     CDS             7354..8475
                     /locus_tag="PK125_02040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB36921.1"
                     /translation="MNFLFITNAHEHTPYNKIVKIQREKNAIYGTALNQITFAYKIEL
                     VRSVKPTLIALGSSRVLELREEFFEVPFVNCGGAMNNLEEGRLFLTELFRYHRPEIII
                     LGLDFWWFSDQFTHRKTFDYHRNTGTDLTFGKLIKPYEFLISSKIELRDYISILLFKN
                     DRNHITNFNNLGLIAIKRSDGFRKDGSYLYANAITGFAPKGQRITGFNDRLDSKRRFE
                     FGADISQNRIAILNEILKLCMNYNVKPILIIPPVTTFVYQRILSRPEKYGYVFKFQEY
                     VKSLPFENYDYHDIRKAASCNCECIDGFHIGDVAYQKILLDILKQNHDSVIAAFVNNN
                     LLERTVRQNEGKVMTIFPNDEGKFNYEETDFLELGCRKN"
     gene            complement(8490..10037)
                     /locus_tag="PK125_02045"
     CDS             complement(8490..10037)
                     /locus_tag="PK125_02045"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HPB36922.1"
                     /translation="MDSQQKVLNIFPLPWIVVLLSFTIIIRLPVLFIAGIIHNDSYEY
                     IRAAKSMLQGDWTGGVAPPVYSFCIMVLNKLVGNYELAGVLVSWIFGAAVVVPIYFFA
                     RKVFDERTGKIASLLSVVQPTLYFYSGSVLSDSVYYFFIALSVYLGWMAFEKGKLITV
                     VLFSLSTTISYLTRPEAIGFMFIFFLWILLINPPGEKRNLLKRLTLAVTAIIFFIIFS
                     SPYLVALRKEFGRWEISRKASISIEMLEKNTNTIIQQDKETESGPEIPTNKRKISIKS
                     IVREPVSISKKLLAGFVLALFKYQQALNPVLFLLALIGLVRRRHDRTLWRIDLYILSY
                     FFFFFAMVLPFFWITKRHTSHLIVIALPWAAYGLIRISDFVRNKTTIAFMRNHVGTIF
                     LIFIMIILFTQGVVERMNSRKHRVIRKEVGIWMKNNLQKGPVISRLPHEAFYGDMDWI
                     NVQDYSYDNLLEKATDKQAKYLIIDMDVMADNTNFVNRLKENNFDLIYVKQKRNQKIF
                     VFSFQER"
     gene            complement(10886..11335)
                     /locus_tag="PK125_02050"
     CDS             complement(10886..11335)
                     /locus_tag="PK125_02050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB36923.1"
                     /translation="MNIGVQPGIVVVDSQLWTIESHSACHDKYISPYILLRKYGTNIP
                     VCGSDIYLEEVFVCEESINTYRCAAGNDLVCKDYHSHRHTIAYMAKKKDCAACELKIR
                     CAKQSLEIYKTRQRFMERFCKRNGQNDFDLAGWRGLWKDVIQHILSV"
     gene            complement(11431..11688)
                     /locus_tag="PK125_02055"
     CDS             complement(11431..11688)
                     /locus_tag="PK125_02055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB36924.1"
                     /translation="MGLEFPHEAIRCKLASLAGVEDRGVPYFCSASSRVSMQKESSRV
                     FGYPPDNTFLLAQSMEETRLQKPFAMGMQVIYRDTTRNLLR"
     gene            11668..11865
                     /locus_tag="PK125_02060"
     CDS             11668..11865
                     /locus_tag="PK125_02060"
                     /inference="COORDINATES: protein motif:HMM:NF025063.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integrase core domain-containing protein"
                     /protein_id="HPB36925.1"
                     /translation="MGKLKTHGIKISMDGQDRWRDNIIIERFWKTVKYEEIFLKAYAS
                     ISHAKKELMKFFDRYNTRRPH"
     gene            12080..13645
                     /locus_tag="PK125_02065"
     CDS             12080..13645
                     /locus_tag="PK125_02065"
                     /inference="COORDINATES: protein motif:HMM:NF013799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HPB36926.1"
                     /translation="MNSTKKVMVIGLDGMSLSLTSHLIDLGIMPNLKALFKRGTYGKL
                     RSVIPPITPAAWTSFQTGKYPNKHGVVNFFVEKPWTYEYEFTNSTCIVGKTLWKILSD
                     ADKKPIIVNVPLTYPPEAINGIIIPGFDTPETSDDCIHPRGLLKTIENRIGSYDFYRM
                     WWNDAVFKQNGLAGLIRELLQITDSQVEGVKFLMKEFDWNFFMYHFQVTDALQHHAFH
                     LIDPSRHGLDMAEKEQQKIILEFYENIDRKIGELLEGIDRDTCVCVLSDHGFLSLHKA
                     FYLNHWLYQQGYLVKNNRYFLIKQLDNLVHLSKKFKLPFLRNMKYPLRKNPIRTLRKI
                     DYKRTQAYAYCYLVNYAFLFLNKNLKINEEALKKDLKEIQYNGEHIVRNVYRWYTGEA
                     SSDFNPDLVVECIEGYSIMQKIPSKKRPFAFDLSNDSNHAINGMFCLAGSNIKKQHTA
                     VAEIVDVFPTILHLLDIPIPDDIDGTIITDAFESYEGTRYVTSDMSPTSYIEAGTEDF
                     EKVANRLKDLGYF"
     gene            13788..14810
                     /locus_tag="PK125_02070"
     CDS             13788..14810
                     /locus_tag="PK125_02070"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HPB36927.1"
                     /translation="MNNVLDLQQRFRRNIYKYVNGDFLSWKDTLLSTISPSVPFYRHA
                     KRIFHQYMKSYSFSSRGLSPDIVTIDAYQGKNPHVLRMMDFICDLKEDLVGAYLHGSL
                     GTYEETPFSDFDALVILRNEIFKDPERLARTAYIIYCAQSIMFDFDPIQHHGWVVLSE
                     ADLESYPQDRFPKELFHYAKSLFPNHGRSLSISCKNSAQNVRQSFDNLCQSALKRIEQ
                     RESIRNVYELKILLSQFMLLPSLYVEMRDGKGIYKKFSFEAARVDFETKDWSIMDDVS
                     SIRANWSYHISPSFHRLISKPTKHSRLLAKKFSPPVPKEIQRFLTDDFYQRMSRLITL
                     MASKPR"
     gene            14807..15787
                     /locus_tag="PK125_02075"
     CDS             14807..15787
                     /locus_tag="PK125_02075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB36928.1"
                     /translation="MTFRFKNTPQFIPLEVYENEITTMIERLNEHKNIVSVYQVGTVQ
                     HPGISDIDMLVVLKDDAEFYQNPLKNSSVTGRYLFVHPLLGVTKTDFMEAQHFNFFRN
                     WRLLLGEQLITGENKFSSDEIACLQIQIALEYLLSNYIQLTVMKLHKIVNIRALLLNM
                     KAMLYDLRLLNVSSGPLYDLLERLVAWRDRWFEEQPHYKDLTRWINLCYLELGSFLQK
                     QLQMHRFYLPKWGNLHVTKNVVLSPNESFSCKCQGMPLPVAFAFLGKKYLKLQRKLNK
                     VTIFLPIQREKIPSILIRKFNLESKMVQFNLDKPFLTLRSTLNFLRKVHR"
     gene            complement(15821..16921)
                     /locus_tag="PK125_02080"
     CDS             complement(15821..16921)
                     /locus_tag="PK125_02080"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HPB36929.1"
                     /translation="MRVAYFIRNIGVSGGVKVVLQHVQMLKEDGYDVTLFTNRVKDSW
                     DLKVKPVVFDPESFRYLSDFDIIVASIYHDVKMLFSKGIRKLVHLCQGYEPIDYLSRI
                     SGESVTERYQRKGFLSLLESRLDILKFRKRIRQIEAVYALPTFKAAVSKHLVELLESK
                     YHQKCALIQNGIDLNIFHPYPEKVWGANRKIRILSVGSADVGFKGIVDTLDAVEFLKQ
                     KGIEVELIRVSPGIPSRREKEGDLVHQFLTGVKEKEMAELYRDADIFISSSLEGEGFG
                     LPAIEALASGVPSILTDISTYRNFNENKDFAYFVPTHSPEKIAEGIESFIRNVEFRNK
                     CIENGLRVAHAFSLERTKNDLIKFMRDLHSEN"
     gene            17160..18623
                     /locus_tag="PK125_02085"
     CDS             17160..18623
                     /locus_tag="PK125_02085"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family O-acyltransferase"
                     /protein_id="HPB36930.1"
                     /translation="MDLYSLQFFVFLFIVLAIYCLGHQRFRWIILLGASYYFYGTFKF
                     QYLFLLAFSTIVAYFVALLMEKQMSPSGKRMLLLAGCICNLGVLFVFKYFNFFSSSVT
                     GIFGDPRHSSPNHLLRLVVPVGISFYIFQVVSYMIDVYRGDKTAERHIGAFALYVSFF
                     PKLLAGPIERAKQFLPQLHEYHKWDWERATNGFKLMVWGLFKKLVVADRLAVFVDMVF
                     NNPAAHMGPSFVLVAVLYSFQIYCDFSGYTDIAIGVSQVFGFKLTDNFNRPYTARSVA
                     EFWRRWHISFSTWLRDYLYIPLGGNRVVPTRLYINLILVFLVCGLWHGASWTFVAWGF
                     IHGIFIVSGLISRNLRFKVNHAFGLDSHPALQKGFQNCITFALVSVAWIFFRAESLHD
                     AVYMLTHLHTGWVDILKGNGFESMILLGKAKSQLIIALASLTFVWVIHFIESHETMRH
                     MLSEKPFYVRWPIYYLMLSAVFLLSAPGSQKYIYFQF"
     gene            18636..19727
                     /locus_tag="PK125_02090"
     CDS             18636..19727
                     /locus_tag="PK125_02090"
                     /inference="COORDINATES: protein motif:HMM:NF028132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB36931.1"
                     /translation="MNGYRAKYLLFFLPFIAAICLELFVLPIHQFTFRVWESLSVKRS
                     YGILKGQFYPNMKIKKIEDGDLAYYTRCAVKKDVLWITDKFGYRKANVATKKHPIVIV
                     GDSNIAGSSLSQEELLSEVLEKKLGQSVYPLAAERLRALFKHDLIRENLPDIVVLAGI
                     ERDLPESLRPLSAKDLRTPSTLEEALKKLCLSPALQNTAVLVDRIFKANMLNYLRARI
                     NRSGSMSQPELTSAQCPIFFIQGVNANKDSTSERIEQAAQRLKEYRDLLHKKRVRFIF
                     LPIPNKETILYQYLGTQQPLFLEQLIKKSQNLGIEVIDTQKTFHDFYQATHTFPYHAD
                     DSHWNGLGVKIAADLLEKQIMTKPLSIFR"
     gene            complement(19714..21015)
                     /locus_tag="PK125_02095"
     CDS             complement(19714..21015)
                     /locus_tag="PK125_02095"
                     /inference="COORDINATES: protein motif:HMM:NF039907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HPB36932.1"
                     /translation="MEKDHILNRIRDEFDSYELFFLKEKTKKFESRDRGLHGVEIHEE
                     EGISLRGIKQGKMIFTYTFQKGKKAADLLIENTKLLSPFVEIDASAVLPSPYTNYSSV
                     DLFDPDGLAIDDSEKIARLIEMESLILDYDKRIVTTRNCELQETAIEIEIFNSNGLSV
                     RNRKTLYSVFALCVAKDYDEVSWYDWKWSHSFADIDMNALGFEVAQKTISFLDSEQIA
                     TGIYDGILTPQASCEILSVLSGSFLAESLYKNKTKLKDKIGERCFSGNVTLVDSGLIG
                     MGASPFDGEGVPSQENVLVEKGVFKTFLYDTYYGKKLNKSSTGNAARTGLKEPPLCGR
                     RGFYLEGGSDNVCDMMTDGVVIEELMGTHTANPITGDFSLGTVGHLVRGGKKGPFKGV
                     IFSGNIFDILNNVQGVGDDLKFYGTCGSPSVFIKGMKISGK"
     gene            complement(21064..21513)
                     /locus_tag="PK125_02100"
     CDS             complement(21064..21513)
                     /locus_tag="PK125_02100"
                     /inference="COORDINATES: protein motif:HMM:NF005840.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HPB36933.1"
                     /translation="MLRKASIKDIKSIHSIVNSAAAKGEMLARSLGELYDNMRDYFVY
                     EERGKVIGAGALHICWEDLAEIRSLCVTESSRGKGIGRMLVEACMEEARELNIAKVFL
                     LTYQEEFFGKCGFTLVDKKELPQKIWSDCIRCPKFPECDEIAMMRRI"
     gene            complement(21491..23110)
                     /gene="recN"
                     /locus_tag="PK125_02105"
     CDS             complement(21491..23110)
                     /gene="recN"
                     /locus_tag="PK125_02105"
                     /inference="COORDINATES: protein motif:HMM:TIGR00634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HPB36934.1"
                     /translation="MLTFLKVKGFAIIDELQIEFSDGFNVITGETGAGKSIIINALST
                     LMRTKVSSDIIRTKADQAEILGHFFYKDQEFILKRIVSTHGRSRSFLNGEPITTTRLS
                     ELGDTLINIYGQNEFQYLLDRENYISILDSLLYLDNERTTLSEKVQTLRRCESELSAK
                     KREIEGQEKEMALLDFQIDEIDQAALQENEEERVKENLKIIKEAEKIQASLNAIYEGL
                     YEGEESVHGVIKRFTGLLKPFRDIDTLQGLMERIETISYDMEDISLQIRELEKGIVFD
                     PGELQRLDERLQRIYELKSKYGKSYGEIKKYAASARERLAYLKTLTTDIDGLEKKRTS
                     LAQEVQHLAQRLSEERKKGTKSVEKAIVNELGFLSMKGMQFHMEISNKGTIDENGMDD
                     IDFLISTNPGEPLKPLRKIASGGELSRIMLAIKRVIGGDEGKTLIFDEVDAGIGGRVA
                     DLVGKRLQDLASKHQVICITHLPQIATYADRHFLVEKVFVKGSARTEIKQIDNLERVQ
                     EVARMMGGATITEKTIQRAEEMIRHATKGIH"
     gene            complement(23113..23964)
                     /locus_tag="PK125_02110"
     CDS             complement(23113..23964)
                     /locus_tag="PK125_02110"
                     /inference="COORDINATES: protein motif:HMM:NF039482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HPB36935.1"
                     /translation="MNNIHVICKRQKEDAISLAARIIQLYGHKIDVFVDEESARQLNY
                     TKQLEIEHVGEGAKLIVVLGGDGTLLSVARNLKGKDVPILGVNLGGLGFLTEISPEEF
                     PEMLERVISGDYDISQRIMLSVTVKREKEKVFEFAVLNDAVITKDALARIIDIETHVN
                     GEYLTTFKSDGLIFSTPTGSTGYSLAAGGPILYPSMKNIIVTPICPHMLTNRPIILPE
                     KVNIKAILKSKDEKVVLTLDGQIGFPLEFGDEMLIKESSHVVSLVKSSSKGYFEILRT
                     KLKWGER"
     gene            complement(23974..25002)
                     /locus_tag="PK125_02115"
     CDS             complement(23974..25002)
                     /locus_tag="PK125_02115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB36936.1"
                     /translation="MDRRLGFCGNLSTTAMGILPHENIDDALELALSLDIPFWPQLPK
                     LSFSEDMYVQAMEYFPGMVIDEDRQQIYFDTNRFINDIPLYIELEKDPTTFILSERFS
                     HVYQRFLSLELDSFKAIRGQIIGPISLMLKIVDENGKPIAYNDEVRAFAYTIIQKKLN
                     AQYAQLRAKNEKAFVWIDDPGLEFVFNAMCGYDNVKAKEELTVFFDGVEGPRGLHLCG
                     RPDWDFLLSLNIEILSFNAYAFGEIFVTYDRVKDFLGKGNIISWGIVPTYFEEFSKED
                     SSGLAGRLESMWAVLERKGIDRELIIQNSLLAPATCNLLNADKTTTVEKSFKLLTSVS
                     SHLKEKYL"
     gene            complement(25069..25209)
                     /locus_tag="PK125_02120"
     CDS             complement(25069..25209)
                     /locus_tag="PK125_02120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB36937.1"
                     /translation="MIQRFFAGIILSSELLLKIPLADWVDFVYGQYVDVNRKEIQVVR
                     VL"
     gene            complement(25285..25968)
                     /locus_tag="PK125_02125"
     CDS             complement(25285..25968)
                     /locus_tag="PK125_02125"
                     /inference="COORDINATES: protein motif:HMM:NF012780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HPB36938.1"
                     /translation="MFLYRYLLRLGNRNVNDIMAPRPDIISIDVDDSWEEISKKIKTC
                     GYSQSPVYRQDPDNILGIVSIKDIWRQPSDDQVKGIQGIIQQPLFAPESLKALKILEL
                     FKQSRTHVALIVDEYGAIQGIVTLHDILEAIVGDLPSDELTEDSNVVPRAYGSWLVDG
                     RQSILTSLKRFFKLAPCPMKIQAVTTRLRVSGCFLLIVFLQQGIILTLPAHDLKSSIW
                     MVTESIKFL"
     gene            25995..26243
                     /locus_tag="PK125_02130"
     CDS             25995..26243
                     /locus_tag="PK125_02130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB36939.1"
                     /translation="MLLEIPERNLPEETVLNPERSVQIELTNHEILNVNLFMEMPQER
                     SRVSVYFNFSPESIYLCGKEKDFILNKSFVFSVRDLDL"
     gene            26788..27402
                     /locus_tag="PK125_02135"
     CDS             26788..27402
                     /locus_tag="PK125_02135"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HPB36940.1"
                     /translation="MQLGSEFKILSILVTTIIAITFIALTSVSASGGEGGHENIITNE
                     IIQYLKNKKVRASDEKLKKIAMSVYEEAKDNEIDYRLVLAVMKVESNFRSDAISKKGA
                     RGLLQIKPSLAKYVSKEMGVSIKSPKCLDEPEKNIRIGVSHLSWLIEKFENLNAALHA
                     YNVGIGKAKKVASEDDGPDTHFTRKVLAEYRLIKNALPHSHPEQ"
     gene            27574..27792
                     /locus_tag="PK125_02140"
     CDS             27574..27792
                     /locus_tag="PK125_02140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB36941.1"
                     /translation="MPKQKHFQEPLFQELKKDYCYVCYERIKKDEGIYIGNGMWRHKK
                     CKPGSNRWLKSRVGQENPLLSSCDQSTK"
     gene            complement(27809..28654)
                     /locus_tag="PK125_02145"
     CDS             complement(27809..28654)
                     /locus_tag="PK125_02145"
                     /inference="COORDINATES: protein motif:HMM:NF014679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HPB36942.1"
                     /translation="MKIITDSTADLPKELIIKHNIGIVPLTIHLGDRSWKDYYDVEPD
                     EYYRLLRKTTAHPTTSQPTPQHFIDAYSPFVEKGESILSIHLSSKLSGVFQSALLARS
                     HFPGAMIEVVDSLQASFALSILVIICAETANRGASLSEVAHVAREIGPQIETYFSVDS
                     LEYLHRGGRIGKAQVFLGTLMKIKPLLKVMNGEVQPIEKIRTSERLLNRFVELVELAV
                     QEHSQLLMAVGESDNAEVMTDLLGRLERIPGISLMFRGKLGGVITSHAGPGALGIAYV
                     KNVLK"
     gene            complement(28670..30292)
                     /locus_tag="PK125_02150"
     CDS             complement(28670..30292)
                     /locus_tag="PK125_02150"
                     /inference="COORDINATES: protein motif:HMM:TIGR03599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HPB36943.1"
                     /translation="MKPNEKKKTIAYLSAKRFKLCILAGAESVFKNRDHLNKINVFPV
                     PDGDTGSNLSMSLMSVVREIEALQDPSLETIVKATAWGALMGARGNSGIIFAQILSGI
                     AEGIEGKERLSAKDVALSFSHAVKKAYKAILYPTEGTILTVVRETAEAAIKAAENTSD
                     LYFLLHAMVTAARTSVERTPEMLPKLKEAGVIDAGGLGFFYFLEGMLRLVDGIAMDDT
                     TVDNEKMLVDMQKETSTSQPSNRYCTEFILKGSQISEDHLKKDLSQKGDSIVVIGDSR
                     LARVHIHTGQPQKVLEYAFSLGQVSSIKVDDMLTQHTSRFVNHKGAKTSSVVAIVPGD
                     GFKEIFYNAGAELVVDGGATENPSTMDIVSAIEAVESLNVVILPNHKNIYPAALKAAE
                     IATKQVTVLKTSTPSEGISALLAYMDDAPFEENVSRMDMASRQVKSGEILVASRDVTL
                     GGVDVKTNDFIGVYRGEIQCSSASLCETLQVLVKSMYEPSDEIITLYFGEIIRKNDAE
                     AMQSILQQSFPDKSVELYFGGQAHAQYIVSVE"
     gene            30770..31651
                     /locus_tag="PK125_02155"
     CDS             30770..31651
                     /locus_tag="PK125_02155"
                     /inference="COORDINATES: protein motif:HMM:NF020252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HPB36944.1"
                     /translation="MTGDLKIKIQETIDFLPPIPAVMAELLVALNDKDIELEALSHII
                     SKDPLMSMNVLKISNSAFYRLPNKVETIDRAVTLLGIKEITGICIACGTYQALKPSKN
                     VETFDLHDFWRHSVTTGVVARRLSEELGILDHNVLYFLGLLHDVGKVILDRVAHETYK
                     VVIQETLQENITIREVEKRLIGESHDVVGAWLMEKWRLPITFVDVAKYHHSVNETPED
                     NRAATAIISFADRLSWIHYAGLESNFDVTLLTEHGGYLFLEEVTDIIDEVDLGKFMRE
                     LDSANDEIDEMMKILQL"
     gene            complement(31697..32332)
                     /locus_tag="PK125_02160"
     CDS             complement(31697..32332)
                     /locus_tag="PK125_02160"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005777 - peroxisome [Evidence IEA];
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003986 - acetyl-CoA hydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0008413 - 8-oxo-7,8-dihydroguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_function: GO:0010945 - CoA pyrophosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035539 - 8-oxo-7,8-dihydrodeoxyguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0009132 - nucleoside diphosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0015938 - coenzyme A catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HPB36945.1"
                     /translation="MFEFTIDMIEAQLQKPLPGVKAQLRMAPRPGSLKSPAPESKPRK
                     SGVLVLLFPSVETGELCFVLTRRTENVADHKGQISLPGGAVDPHDHSIADTALREACE
                     EVGVRIDTMRILGPLTPIYIPPSDFCVQPYVACMFQSPQFLPQPEEVAEIIEVPLRYL
                     LDERNVVIEEWPLDHEIKQVPYFNVHGHKVWGATAVVLSEIAAILENLVKT"
     gene            complement(32343..>33083)
                     /locus_tag="PK125_02165"
     CDS             complement(32343..>33083)
                     /locus_tag="PK125_02165"
                     /inference="COORDINATES: protein motif:HMM:NF014910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HPB36946.1"
                     /translation="PAPEAKATILAFFSHVEDTAEAVSGIVAARIVPSTIEFMDQASI
                     RCSEQANPMGIPEDIEGLLLIEIDGDGDAIKTLAQKIKTVLVQHNATDVKLTQDPVEA
                     EKLWQARRVVSQATYNLNPVKIAEDVVVPRSNIPTLIRFLEETGKKHGIPILSFGHAG
                     DGNFHVSLMIKDTKEDYEKAHKAVEEIFSETIRLGGTLSGEHGIGTAKAPYIGMELSP
                     VVIETMKSIKRIFDPNYILNPGKIFVAN"
BASE COUNT         9578 a         7380 c         6467 g         9658 t
ORIGIN      
        1 ggaaatggaa gaaaaagcat tacccatcgt aataacaggc cacattgacc acgggaagag
       61 cactcttatc ggtaggcttc tctatgatac tggaatcttg aaagaagacc gataccaaga
      121 gatcacagca tcttcctcgc tccttggtaa agaaactgag tttgcctttg ttctcgacgc
      181 ccttgaagaa gaacgggaaa aaggtataac catagatacg acacagatct attttcagac
      241 accgaagagg aaatatgtaa tcatagatgc acctggccat aaggaatttc ttcgtaacat
      301 gattacaggc gcctcctatg cagatgctgc cgttctcatc attgactcga atgagggtat
      361 aatggagcag acaaaacgtc atggttatct cctgagtatg gtaggcatca aagacatctg
      421 cgttctcata aataaaatgg atcttgtggg ttactcgaag gacatctatg acagggttgt
      481 tgccggtatg aaggatttct ttggaaatct atcattgcga cccaggttct acatccccat
      541 atcagctctc aaagggaaca atgttgccaa aagggacccc tccatcacct ggtatgaggg
      601 cccatgtctt tttgaggccc ttgatgagct tgaatttaag acctttgaag aacggccatt
      661 tcgttttccg gttcaggatg tctaccgcat tggcaatgaa aagatcgtgg caggaaggat
      721 tgaatcaggc gaagtcgctg ttggtgaaac tgtgtatctc ttgtctaaag gtttaagagt
      781 caagattaaa gagataagga aattcctgga agacaacatt aagactgctt cctatggtga
      841 atcaatcggt ctcattgtag aaggtgcaaa cggaattaaa cgaggcgata ttctttcagg
      901 tgacaacaac gtcaggacag taaaggattt tgatgctaac cttttctggt tctatggaga
      961 atacaacaag ggagacaaga tccagattaa gtgtactaca caggagagct attgttctct
     1021 ctcaataact gaaaagtttg atcctgccac catggacaag aaaaaagagg gtttacatca
     1081 cctcgaaatc ggagaaatag ccaaggcaaa gatcaaacta cgcaagaaaa tagccatcga
     1141 taccttctct tacattcctg agatgggaag gtttatccta gaaaagaatg gcataccggt
     1201 tggtggagga ataatcgtct gatagcattt tagagatctt aagatgaaag agcttttatt
     1261 gcgcggtgtc cgtccgggcc ttcttcatac tctcaagttc gtttcggaga atggcatatt
     1321 gttgcgaaag atcctttaat ctgtttgtga gttttgatat aaccactgaa aaatgaaggc
     1381 ttatgagaat aaggaacata aaggcaaaca agaacagggt tgatacatta gaaaccgcgc
     1441 caataaaatg ggatatccat ttcagcagat caaaccatac cataaggacc aggataccga
     1501 accacgttgc aaaccatagc tgtgaatatt cttccctcag ctttcgtctt ctcacaagat
     1561 ccacaacgag tagaagcatg gcaatacaaa tcacaaacgt gactatttta atacgaaaag
     1621 acatacttac ctcctcttaa aacatctctc tcaccgtctt aaaagtgtca caataataga
     1681 caggaacatc ttaaaaatat aatataacgg tttaagtccg ctgtgcattg acttgtcatc
     1741 agggctaggg aacatcctta caggaacctc ttttattctg aaccctgccc gatgaagcat
     1801 aattattacg tcggcatctg gatagtcaat aggaaatttg tcaccggcaa gaagatggaa
     1861 cgctctgcgg ttcattgcct gataacctga tgtcggatcg gtaacctttt gccctgtaaa
     1921 aaatgtggca atagcgctga agagcctcat accgatcatc ctgaccaccg gtgctctata
     1981 agcaggccaa tcaagacctt ctttctgaga aataagaaaa cgcgacccaa tggtgatatc
     2041 tgcagttcca ctttgaacag ggttaataag atcataaata caggtggggt catgctggcc
     2101 atcaccgtct agttgcacaa gaaaattgta tctatttctt gttgcatata tataaccggt
     2161 ctgaagtgcg ccaccataac ccagactgaa aggatttgag attactgacg caccagcgtc
     2221 aagagccctt gccctcgtat catcactgga tccgtcatca atcactaaca cgtcaaaata
     2281 cgggtaattg gctttaatct gtgtgacaat aggtgcgatg tgggcacctt cattatgggc
     2341 agggatgatc acaattgtcc tttcaagctc attttcgacc atccgacctc accacgcttc
     2401 tattattgtc tggacctgct ttgagttctt ttaaggattt tatcggttgt gctggattac
     2461 ctgccaccca catgtagtct tcgatgtctt tcgttaccac tgaaccaggg ccaataagtg
     2521 caccctcccc tatggtaaca cctggtagga taattgcccc aacccccaca aaagcaccct
     2581 ttccaatttt tacattctca atttttttac caccgacgat atctcgttgc aatggcgtag
     2641 gattgtaatg ggcaagtatc ttgacctccc ttgtcagcca agccccttcc tcgatttcca
     2701 ccatgtctgg atgaatggaa tcgataacca cgtggtaggc aatgaagaca ctccagacat
     2761 tttttatgcg tactcccctc atcttgtgaa ggagtgctgt aataaaagat ggatacggaa
     2821 catgataggc tatcagtccc ataatggctt tataaagatc gccaaaagaa aagaacagtc
     2881 ggtttgcctt tttatattgt tccatcatcc agttatcgtc attcaaaata tcccaggctt
     2941 ttcaagtggg ccttcaattc ctcttcctct tctttggtgt agacctcacc tgttgcaaaa
     3001 ctacatgtat tcaaatcacc tgtttcgata gggttttgtt caagaatctc cttctcaata
     3061 gcatccttta gtaccatgcc gtccatgtca gggtcaacgg gtaaacccat aagatataga
     3121 atggtcggtg caatgtccat gatattacca tttataatcg tggagttact ctttaaccca
     3181 gcacccttca ccatgagaat tccatccatc ctgtgagagc tggaaagtcc acgagagtaa
     3241 tcaagggtac gatgggaggc gaattcatac tcgccaaatg caactatttc aagtcccttt
     3301 ggcaaaaaaa caatatctgg tgccgttaat gtgtgaggtc cagaatagag ttcttctttt
     3361 ctgtaaatct cttcgatgac atgatgtcct gtctctgggt ctgtaagacc cctcaattct
     3421 ttaattattt cttcccttac agctttgtat tcttcgcctc tcctgataat tccccctggc
     3481 tctctccctt ctaagtttac atatatttgg cctgctgtgc ttccaatagc ataggctttt
     3541 gaagtacccc aatcaacatc ctcgaaggag agaaaaaact tcttaagcca tgaagaacct
     3601 tgaccgaagc gagtcttgtg ctttagtcgt cctagaccaa ctgaaagtaa aatatggtag
     3661 aaggtcatgg gtgacacacc aaggcgaaat aaaaggtgct tgaatcttgt catgaccctt
     3721 ttccttaatt ttaagaaacc ttttttaatg agaaaattat taacataaaa aagctttttt
     3781 aaagccccgg caccgtgatc ggacataaca acgagaatag cattttctct ttcgcaccag
     3841 tccatcacat cacaaagaat actgtccatg tactcataga actggggaat gacatcgata
     3901 taacgttcct cgtattgtcc atgacggaaa tgccgcctat cataagtgtg ccagagttcg
     3961 tgctgtaaaa catcgatccc ctgatacaca gcaacgaaaa gatcccaatc ttcctgactg
     4021 agaagaaaat ccatggcttg cttttgtttt gatgtaacct catacaaatg tttcaaaaat
     4081 cgatcttcct gttttcttga atatgattct gtaatatgga tctggtattt ccctgccacc
     4141 ctatcaagct ctttagctat agaaaaagga tgggtgaaat cattcctacc tgaaggtgtc
     4201 agcattcctg aaaccattat tccgttcaca ggatcaggag gatacgtcac cgggacatta
     4261 aagatacaaa ccttcctgtc agcctctgtc gcaatggtcc agaatgcctt cccgttgcgc
     4321 attcgtgcac tgatgggtga aagttcatag cttccttctt tacggtaaat aaaatcgaaa
     4381 atgccatgtt tgccaggatt cttaccagtc atgaaggaag accaagcaga acctgtaata
     4441 ggaggaattg ttgttctcag cttcccggag gcccccccat ccttaagtgc cttaaatgag
     4501 ggcaacctgc ccctatcgat caacgactca attatctcgg gactcgcacc atctagacca
     4561 agaacaaaaa ccttttggtt caaagccatt tctacaaaat ctccacacag aaaactaccc
     4621 tggaatactt ttaccaggca acttatctac tctcagcaaa taagccgctt ttcaggattc
     4681 ctgatctagc aaacattgcc aggtttattg caagggtaat tgccacaata atcaacgata
     4741 ccagaatcct ttgtccaaca gcaaaagcaa ctgcctccgg atgggaagcg ccgatgaatt
     4801 taagtgcccc ataagatccc atctcaatga cgcccaggcc accaggagtg acattaataa
     4861 aaccggcaag ctgaacaaga gaagcagcaa aaaatacatc aaagaatgtg aatgtgatcc
     4921 cgaataccac tccaacagca tagaaacgaa gggcaataaa gatatacttt ataaaactcc
     4981 acgccaatac agaacatagt gtattttttt ttattggaat atattccagg actgcggcag
     5041 gatcagtcct tttccatctc ttaagaatcg gtaatctcag aaccaggttg atggcacgag
     5101 aatatagaac tattagatat ggaaggatct cgttattctt ccacaaaaat aaaccaaaaa
     5161 ccatcccaac tattccaact gggatgatga gcattcccag tttattttta cccagtaaca
     5221 taagaaaagt tgatataatg atcaccagta gcacaacgac atcaaaaagc ctgtcgagga
     5281 acactgacaa caatgcagta gaggtcttta cctctttgta ttgcttcata aaaaatattc
     5341 ttacaccaac aaggctcaca tcgctgggca caaaaagacc cactgcataa ctattcatca
     5401 gccattgata gaagtgcaaa aaatccagac gcttattggg taattcagac aacagagact
     5461 tcagaacttt caaccaccga atgttatgta taaaagtaaa acaagccgta aagaaaaaaa
     5521 caagacctat cgcaggccgg ctaacaaccg aaatcttgat aacatctgat aatccccagt
     5581 agtagacgcc gatgactaag agaaaaagcg cgagaagcca ctgccacaca acccggttca
     5641 ttcttgacaa gcctttttca ccctgtatct cggctttttt ggtaataaca tgatacacat
     5701 attaccatat tcccttggaa aactgagaaa gaaaagaagg atcacaggat cacataaaga
     5761 tttggcattg atttagtacc tgtttgataa tactatattt ctttatactt aaaaggaata
     5821 tcggtcagtg cttttcaatt catatgtatt tattcttctt tttcttccct taactttatc
     5881 tatttatttc tttttgagca gaaaaaaact gattctggcc tcaaaggcct ggcttgtact
     5941 tgcctctctc tttttttatg ggtggtggaa tccagtctac attcctctca tccttggaag
     6001 catcctcttt aattatggga tagggttgac tcttacaagg gccaagcaat cccaatctag
     6061 gctttttttg atcattggaa ttctaggcaa tctgatatta ttagcttatt tcaaatatac
     6121 ggatttcttt ataaacaata tcaaccatat aaccggcagt cacataagac tgctccagat
     6181 agccctgccg ttgggcatta gcttcttcac attcacccag attgcatatc ttgtggatgt
     6241 atacagacgt atggccaagg aatacagcgt gctcaactat tttctttttg tcacattttt
     6301 tcctcacctt cttgcaggcc caatcatcca ccataaggag atgatgcctc agtttgaccg
     6361 attaaaaaat aagcttttaa ataataagaa ccttgttcat ggtctcctcc ttttttttgt
     6421 tggcctgttc aaaaaggtga ttattgcaga tacccttgct gtatgggtcg accagggttt
     6481 cgaccacgct gtcatcctaa atattgtgga ggcttggact gtctcccttt cctattctct
     6541 tcaactttat tttgacttct caggttatac cgatatggca ctcggttctt ctctcatgtt
     6601 caacataagg ctccctatca atttcaatac tccttacaga tcgatcaaca tacaggaatt
     6661 ctggcgtaga tggcatataa ctttgggcag atttatcagg gattacgtct atataccctt
     6721 gggtggaaac agggtatctg aaatacggat tatcataaac cttatgatca ccttcttcat
     6781 catcggtttg tggcatggag ctggttggac attcgtctgc tggggactga tgcatggact
     6841 tgccattgtg gtacaaagaa cctggaaaag gttgaacata caccttccaa aacctcttgc
     6901 ctggcttctt acattcaatt ttgttaattt ttcatggatt ttttttaggg caagaaactt
     6961 tgatgatgct atcaaagtgg tgaaaggtat gtttggtgcc catggcctgg gggtaaccgg
     7021 ttatatcaac catgcctttg agtcttttaa gacgtttcga gctgctctaa aatattggct
     7081 ggaaaatatt gacggaaata tttacaccct tgttttcatt atcatctgtc ttatgttgtc
     7141 catttatttt cgtaattcaa acgacatgga acagcgattt tctccaacat ggaagaatgc
     7201 ctgtatgctg ggtttacttt cattgtacgc catgcttaat ctgcaaaagg caagtcaatt
     7261 catatatttt attttttaat aaacatgaaa aagtatagtc aatttttaaa agtgtatttc
     7321 ttattcatcg caattatcgc gttcccatta ttcttgaatt ttctttttat aacaaatgct
     7381 cacgaacata caccgtacaa taaaatagta aaaatccaac gggaaaaaaa tgcaatatac
     7441 ggaactgcac tcaatcagat tacttttgcg tacaaaattg agcttgtgcg gagtgtaaaa
     7501 ccaaccctta ttgcccttgg atcttcacgt gttttggaat tgcgagaaga gttctttgaa
     7561 gtaccttttg tcaattgcgg tggagcaatg aacaacctcg aagaaggaag gcttttcctg
     7621 actgaattgt tccgttacca tagaccggaa attataatac taggccttga tttctggtgg
     7681 ttcagcgatc agtttaccca tcggaaaacc tttgactacc atagaaacac aggcactgat
     7741 ctaacctttg gtaaattaat aaaaccatac gaatttttaa tatcgtcaaa aatagaacta
     7801 agggattata taagcattct cttgttcaag aatgatagaa atcatataac aaactttaat
     7861 aacctaggcc taatagcaat caagagatcg gacggattca ggaaggatgg ctcctacctg
     7921 tatgcaaacg ctatcactgg atttgctcca aaaggacaga ggattaccgg ctttaatgac
     7981 aggcttgact caaaaaggcg ttttgagttc ggagcagaca tttcacaaaa tagaatagcg
     8041 atactgaatg aaatattaaa actgtgtatg aattataatg taaaaccaat tttgatcatt
     8101 ccgcctgtta cgacctttgt gtatcagcgc atactatcac gacctgaaaa atacggttac
     8161 gttttcaagt ttcaagaata tgtcaaatcc ttaccttttg agaattacga ttatcacgac
     8221 ataagaaagg ccgcatcctg taactgtgaa tgtattgacg gttttcacat tggggatgtg
     8281 gcttatcaga agattctttt ggatattctc aagcaaaacc atgactcggt aattgctgct
     8341 ttcgttaata ataaccttct agaacgaaca gtacgtcaga atgaagggaa ggttatgacc
     8401 atttttccga atgatgaggg taaatttaac tacgaggaga cagactttct ggagcttggt
     8461 tgcagaaaaa actgataaac caaagcgcat tacctctctt gaaaactgaa cacaaaaatc
     8521 ttctggttcc tcttttgctt tacatatata agatcaaagt tattttcctt taaacgattg
     8581 acaaagtttg tgttgtcggc cataacatcc atatcgataa taaggtattt tgcctgttta
     8641 tcggttgctt tttctaaaag attgtcataa ctgtaatcct gaacattgat ccaatccatg
     8701 tctccataaa atgcttcatg gggtagcctg ctgattacag ggcctttttg aagattgttc
     8761 ttcatccaga taccaacctc ttttcgaatt actctgtgtt tccgcgaatt cattctctca
     8821 acaacccctt gtgtgaacaa aataatcatt ataaaaatga ggaaaatagt acccacatga
     8881 ttacgcatga atgcaatggt ggttttattt ctaacaaaat cagaaatcct tataagcccg
     8941 taggctgccc atggcaaggc tatgactatg agatgagaag tatgtctttt tgttatccaa
     9001 aaaaatggta gaaccattgc aaagaagaag aaaaaataac tgagtatgta gaggtctatc
     9061 ctccagaggg ttctatcgtg tcttcttctt accaatccta tgagggcaag cagaaagagg
     9121 actgggttca atgcttgttg atatttaaat agagccagaa caaatccggc caataatttc
     9181 tttgagattg ataccggttc cctcacaatt gatttgatgc taatttttct cttattcgtg
     9241 ggaatctccg gtccagattc tgtctccttg tcttgttgaa ttatcgtatt tgtgttcttc
     9301 tccaacatct cgattgaaat tgaagccttt ctggaaatct cccacctgcc gaattccttc
     9361 cgcaaagcaa ccaggtatgg agacgaaaat atgatgaaga aaatgattgc cgtaacagca
     9421 agggtcagtc ttttcaatag attccgtttc tcaccaggtg gattgatcaa aagtatccaa
     9481 agaaaaaaaa tgaacatgaa tcctatggcc tcaggacgtg ttaaatatga aattgttgta
     9541 ctcaaactga ataaaactac tgttattaat tttcctttct caaaagccat ccatcccaga
     9601 tagactgata gggcaataaa gaaataataa acagaatcac tcagcacaga accggaatag
     9661 aaatagaggg ttggctgaac gaccgacaga agtgaagcaa tttttccagt tctttcatca
     9721 aaaacctttc ttgcaaagaa gtatattgga accactactg cagctccgaa aatccacgaa
     9781 accaaaaccc cagcaagttc ataattacct actaatttgt tcaaaaccat aatgcagaac
     9841 gaatagaccg gaggagcgac acctccagtc caatctcctt gcaacatgga ttttgcagcg
     9901 cgaatatatt cataactatc gttgtgtatt attcctgcta taaaaagaac aggtagccta
     9961 atgataatag tgaaactcaa caaaacaacg atccatggca aaggaaagat atttaagact
    10021 ttttgttgag agtccatata gttagtttca tgaaccatgc gttaatatag ccacaggtat
    10081 ctttctaaaa caacattttt cctgaatcct ttctgacaga atagcttagg gagataagct
    10141 aacccacctc atgcagcttg actcaatttt tcataaaatc gggtagtatt tttcctgaaa
    10201 tctgacgttt tccaaccact gttttttaaa acctgggtag tggagaccag cccgtgctga
    10261 ttttctgagg gtatctggcg ctaaactcag tgatgaccgg atctacagta cccttgacac
    10321 tctcctgccc catagagatt aattgtatgt acagttccag aaacgctata acaaactctt
    10381 cgagctacct ttgtctcctc ttttataaca ttgaccccac gtattttgag gaggatatgt
    10441 atggcaactc ccgtgtataa aaaaatactg ccgcgacagt catcctgatt ctccccagta
    10501 tgtatcggtc tggtgacagc aaaggaaggg ctgcccgtac catttagatc tttgacggca
    10561 gtcatgcaaa gacgaagaag ttcgttcccg cgctcatctt tgagaaactc tggaaggaac
    10621 gggtagttga cagggtggcc tccaatatca acgggttgtt gtccaacaca acttttgcac
    10681 aggccattat agaaagtcca gagcaccagc taccaaccgt aatatttacc ggtcacactt
    10741 tttctacgga tagagaaacg gtaaatccaa tgctcattcc aaagattcta cctgcagctc
    10801 tgccacagag atgtaattgg tttgaggtaa tgccatgctt ttatcggtct tgttgtgtac
    10861 caaagcaata tctcgatgtt ctggattata cagataagat atgctgaatg acatctttcc
    10921 agagacccct ccaccctgcc aggtcgaaat cgttctgtcc attccgtttg cagaagcgtt
    10981 ccataaagcg ttgacgggtt ttgtatatct ccaggctttg cttagcgcac cgaatcttga
    11041 gttcacacgc tgcgcagtct ttcttttttg ccatatatgc aatagtgtga cggtgggaat
    11101 gatagtcttt acacacgaga tcattcccgg cagcacatcg gtaggtattt attgattctt
    11161 cgcaaacaaa gacctcttcc agataaatat cgcttccgca aacaggtatg tttgttccgt
    11221 attttctcag aagaatatag ggtgatatgt acttatcatg acaggctgag tggctctcaa
    11281 tagtccataa ctggctgtcg actacaacta tcccgggttg tacaccgata ttcaccgtat
    11341 gggcatcaag gctctctaca agaaccttcg tctcttaaaa cctgaatccc ggtcacatga
    11401 tctatcctta ccgcttaaga gggctcgaca ttaacgaagc aaattccgtg tggtatcccg
    11461 atatataacc tgcatcccca tggcaaaagg tttctgcaat cttgtttcct ccatggattg
    11521 ggcaaggagg aaggtattat ctggaggata tccaaacacc cgggatgatt ccttctgcat
    11581 tgagaccctt gaagaagcac tgcaaaaata tggaacaccc ctatcttcaa caccggccag
    11641 ggaagccagt ttacagcgga tagcttcatg gggaaactca agacccatgg aataaaaatc
    11701 agcatggacg gacaggaccg gtggagggat aacatcatta tcgagcgttt ctggaaaact
    11761 gtgaaatacg aagagatctt cttgaaagca tatgcatcaa tcagtcatgc gaaaaaggag
    11821 cttatgaagt tttttgatcg gtacaacaca cggcggcccc actagggtct caacgacagg
    11881 attccggatg atgtgtactg gcaaaccctg ccggaagtaa aggatgcgat ataaccatga
    11941 ggacgagcca ctgaaaacct cctctaaact gcccaaaaga tcttgaccat ctcttacacc
    12001 tataaatgtc ttgatgttat ttgccatttc catcatacta taacttcgtt tttatttaat
    12061 tgattataca ggtaattata tgaattcgac taaaaaagta atggtaattg gccttgatgg
    12121 aatgtctctt agcctcactt ctcaccttat tgatttagga attatgccga atctcaaagc
    12181 ccttttcaag cggggaacgt acggaaaact gaggtccgtt attccgccta taacacctgc
    12241 agcatggacg tcattccaga caggcaagta tccaaacaaa cacggggtcg taaatttctt
    12301 tgtggaaaaa ccatggactt atgagtatga gttcacgaac agcacctgca tcgtcggaaa
    12361 gacactctgg aaaatcctga gcgatgcaga taaaaaaccg attatcgtga acgtccccct
    12421 gacttaccct cctgaagcca tcaatggaat cattatccct ggctttgata cacctgaaac
    12481 gtctgatgat tgcattcatc ccaggggcct cttaaaaact atagagaatc gtatagggag
    12541 ctacgacttc taccggatgt ggtggaatga tgctgtcttc aagcagaatg gcctagctgg
    12601 actcatacgt gaactgcttc aaataaccga cagccaggtt gaaggagtca agtttttaat
    12661 gaaggaattt gactggaatt tcttcatgta ccattttcag gttaccgacg cattgcaaca
    12721 tcacgctttt cacctcatcg acccatccag acatggtctc gacatggccg aaaaagaaca
    12781 gcaaaaaatt attctggagt tttacgagaa tatcgatcga aaaataggtg aactccttga
    12841 agggatcgac cgggatacct gcgtgtgtgt tctttccgac catggatttc tttcattgca
    12901 caaagcgttc tatcttaacc actggctata ccaacaggga tatcttgtga aaaacaatcg
    12961 ctattttctt atcaaacaac tggataacct tgttcattta tcaaaaaaat tcaaacttcc
    13021 atttttgcgc aacatgaaat accctctccg gaaaaatcct attcgcaccc tccggaaaat
    13081 agattataaa cggacccaag cttatgcgta ttgctacttg gtaaattatg cgtttctctt
    13141 cctgaacaag aacctgaaga tcaacgaaga ggcattaaaa aaggatttga aggaaataca
    13201 gtacaatgga gagcacattg ttaggaatgt ttaccggtgg tataccggag aggcatccag
    13261 cgatttcaat cctgatttgg tcgttgaatg tattgaggga tattcaataa tgcaaaaaat
    13321 accgtcaaaa aaacgccctt ttgcattcga cctatccaac gatagcaatc atgcaataaa
    13381 cggcatgttt tgcttagcag gcagcaatat caagaaacag catactgcag ttgcggaaat
    13441 agtcgatgtc ttccccacca ttcttcacct gttggatatc cctattcctg acgatattga
    13501 tggaactatt ataactgatg cttttgagtc atatgaaggg actagatatg tgacctctga
    13561 catgtcacca acctcttaca tagaagcagg cactgaagat tttgaaaaag ttgctaatcg
    13621 tttgaaggac ctgggttact tttgaatgca cgtaaaacat ttcgcaaatt taagttgtcg
    13681 tgaacttgag ataaggtacc tcatattacg atagcaggca taatcccagt gtcagggtgg
    13741 attatgaaag tcttatagat ctgggtttta atgagaaggg actatttatg aataacgtgt
    13801 tggatttaca acagcgcttt agaagaaaca tatacaaata tgtgaacgga gattttcttt
    13861 cgtggaaaga tactttactt tcgaccatat ctccctcagt tccgttttac agacatgcaa
    13921 agagaatctt ccaccaatac atgaaaagct attcgttttc gtctagaggg ttatcacctg
    13981 acatcgtgac gatagatgcc taccagggca agaaccctca tgtgctccga atgatggatt
    14041 ttatctgcga cttaaaagag gatcttgtgg gagcttatct ccatggcagc ttgggaactt
    14101 acgaagaaac ccctttcagc gatttcgacg cgctcgtaat cttgaggaac gaaatattta
    14161 aagatcctga aaggcttgca cgcactgcct atataattta ttgtgcccaa tcgatcatgt
    14221 tcgactttga tcccatacag catcatggat gggttgtcct ctcagaggca gacctggagt
    14281 cttaccctca agatcggttt ccaaaggaat tgtttcatta tgcaaaatcc ctcttcccta
    14341 atcatgggag gtcgttgagc attagttgca aaaactcagc acagaacgtt aggcaaagtt
    14401 tcgacaattt atgtcaaagt gcattaaaga ggattgagca gagggaatct atacgcaatg
    14461 tatatgaatt aaaaatcctt ttaagccagt ttatgttact gccaagtctc tatgttgaga
    14521 tgcgtgatgg aaaaggtata tacaagaagt ttagttttga ggcagcccgc gtcgattttg
    14581 aaaccaagga ctggtcaatc atggatgatg tgtcctcgat acgtgcaaat tggtcttacc
    14641 atatctcacc ctcctttcac cggttaatat caaagccgac aaagcactcg aggcttcttg
    14701 caaaaaaatt ttctcctccc gtacccaaag aaattcaaag gttcctgaca gacgattttt
    14761 accagagaat gtcgcgtctc ataacattaa tggcaagcaa acctcgatga ctttccggtt
    14821 caaaaacaca cctcagttta ttcctttaga ggtttacgaa aatgaaatca ctacaatgat
    14881 tgaaaggttg aatgagcata agaacattgt atcggtctat caagtgggaa ctgtccaaca
    14941 tcctggcata tctgatatcg acatgttggt ggtcctcaaa gacgatgcag agttttatca
    15001 gaatcctttg aaaaattcat ctgtgactgg gcgctatcta tttgttcacc ctcttctagg
    15061 tgtaaccaaa actgatttta tggaggcgca acattttaat ttctttcgta attggcgtct
    15121 tcttttgggt gaacaattga ttacagggga aaacaaattc tccagtgacg agattgcttg
    15181 cttacaaatc cagattgccc ttgaatattt gctttccaac tatatacagt tgactgttat
    15241 gaaattacac aagattgtca atatccgagc ccttctgtta aacatgaaag caatgcttta
    15301 cgaccttcga ctactcaacg tttcatctgg acctttatat gatcttttag agagacttgt
    15361 tgcttggcgt gataggtggt ttgaagagca accacattac aaagatttga ccagatggat
    15421 caacttatgc tacctcgaac ttggttcatt ccttcaaaaa caactgcaaa tgcatcgttt
    15481 ttatcttcct aaatggggca accttcatgt cacaaaaaac gttgttctat ctccaaatga
    15541 gtctttttca tgcaaatgtc agggaatgcc actacctgtt gcatttgcct ttctcggaaa
    15601 aaagtatctc aaacttcaac ggaaactcaa taaggttacc atcttccttc ctatacaaag
    15661 ggaaaaaatt ccttccatac ttattcgtaa gtttaacctc gaatcaaaga tggttcaatt
    15721 caatcttgat aaaccatttt tgactctgag gagtacactc aattttttgc gaaaagtaca
    15781 tcgatagagc cacgagacaa gtattaggct caagcctcag ctagttctca ctgtgcaggt
    15841 ccctcataaa tttgatcaaa tcattttttg tcctctccag gctaaatgca tgtgcaacac
    15901 gtaacccgtt ttcaatgcat ttatttctga actcaacatt cctgatgaag gattcaatgc
    15961 cttcagcaat cttttcgggg ctatgagttg gtacaaaata cgcaaagtct ttattttcat
    16021 tgaagtttct gtaggttgaa atatccgtaa gtatagatgg aaccccagat gctagcgcct
    16081 caatggcagg gagaccaaac ccctctcctt caagagacga agagataaag atatctgcgt
    16141 cacggtagag ttctgccatt tctttctcct tcactcctgt aagaaactga tggacaagat
    16201 caccttcttt ttctcgtcgt gatggaatac cgggcgatac ccttatgagc tcaacctcga
    16261 tacctttttg tttcaagaac tccacagcat caagggtatc aacaatacct ttgaatccca
    16321 cgtctgcaga acctactgat aaaatacgaa tctttctgtt ggctccccat accttttcag
    16381 gatatggatg aaagatatta agatcgattc cattctggat gagtgcacat ttttgatggt
    16441 acttgctctc taggagctcc acaagatgtt ttgatacagc ggctttgaat gtgggcagag
    16501 cgtagaccgc ttcaatctga cgtatccttt ttctaaactt caatatatcc aaacgacttt
    16561 ccaacagaga aaggaaacct tttctctggt aacgctcggt gaccgattcc cctgaaattc
    16621 ttgaaaggta atctatgggt tcataaccct ggcacagatg tactagtttt ctaatacctt
    16681 tggaaaaaag catttttaca tcatgataaa ttgatgcaac tataatgtca aaatcagaca
    16741 aataccgaaa gctctcaggg tcaaagacaa ccggctttac ctttaagtcc catgaatcct
    16801 tcaccctgtt cgtaaataaa gtaacatcgt agccatcttc cttcagcatc tgtacatgct
    16861 gcaagactac cttgacgcct ccagacacac caatgtttct aataaaataa gctaccctca
    16921 tggaaccacc aacactgctt taaaacatac agtagtttaa ttcaaagttc ggtggcatct
    16981 gccacacttt aatatatcag ctcttccctg ctatgtgcaa ttagagaatt atggagtgta
    17041 cattcaacgt agattttgat gcaatttcat atgcttgtat ctcctgaaaa cctgatcccc
    17101 ttttcgcttg ctatccagcg agaagtttag taaagaagtt aacatttgcg agtttcataa
    17161 tggatttata ctcacttcaa ttcttcgtct ttctttttat cgttctcgct atctattgcc
    17221 ttggacatca acgattcaga tggatcattc tgctcggagc aagctattat ttctatggta
    17281 cctttaagtt tcagtaccta ttcctcctcg ccttttctac tatagtagct tattttgtgg
    17341 ccttactcat ggaaaaacag atgagtccat caggcaagag aatgcttctt cttgcaggct
    17401 gtatctgcaa tttgggtgtt cttttcgtat ttaaatactt caacttcttt agcagttccg
    17461 ttactggtat tttcggtgat ccacgtcatt cttctccgaa ccatctgctc cgacttgttg
    17521 tccccgtggg aatctcattc tatatattcc aggtagttag ctacatgatc gatgtgtacc
    17581 gaggagataa aactgctgag aggcatattg gtgcgtttgc tctgtatgtt tcttttttcc
    17641 ccaagcttct tgcagggccc atcgagcggg caaagcaatt tctgccccag cttcatgaat
    17701 accataagtg ggactgggaa cgggcaacga atggatttaa actcatggtt tgggggttat
    17761 ttaaaaagct ggtggtcgct gatcgccttg ctgtgtttgt cgatatggtt ttcaataatc
    17821 ctgctgccca catggggccc agctttgttc ttgttgctgt attatacagc tttcagattt
    17881 actgcgattt ttcaggctac actgatattg ccataggagt ctcccaggtt tttgggttta
    17941 aacttacaga taattttaac cgtccttaca ctgcccgttc tgtagcagag ttttggcgac
    18001 gctggcatat ctctttttcc acctggctta gagactacct ttatataccg cttggcggaa
    18061 accgagttgt tccaacacgg ctttatataa atttaatatt ggtattcctt gtctgtggtc
    18121 tatggcatgg tgcaagttgg acctttgttg catgggggtt catccatggc atcttcatag
    18181 tgagtggact catttcccgg aacctgcgat ttaaagtcaa ccatgctttt ggtctcgaca
    18241 gccaccctgc tcttcagaaa ggttttcaga attgcatcac ctttgctctt gtctcggttg
    18301 cctggatttt tttcagggct gaatcactcc atgatgcagt ctatatgtta acacatctgc
    18361 acacagggtg ggtggatatc ctcaagggga atggatttga atctatgatc ttacttggga
    18421 aagcaaaaag tcagcttatt attgcccttg catcccttac ctttgtctgg gttatccatt
    18481 ttatcgagag tcatgagacc atgcggcata tgttgtctga aaaaccattt tatgtgcgct
    18541 ggccgattta ttatctgatg ttgtccgcag tgtttctgtt aagtgcgcct ggttcacaga
    18601 aatatattta ttttcaattt taggaatcag tggccatgaa tggttatcgc gcaaaatacc
    18661 ttctgttttt tcttcccttt atagctgcta tttgtcttga gctgtttgtt ctccctatcc
    18721 atcagttcac cttccgagtc tgggaatccc tttctgttaa acgttcctat ggtatcctga
    18781 aaggtcaatt ctatccaaat atgaaaataa aaaaaataga ggacggagac cttgcatatt
    18841 acactagatg tgcagtgaaa aaggatgtat tgtggatcac agataaattc ggttacagaa
    18901 aagccaacgt ggcgacaaaa aaacatccaa ttgttattgt cggggattca aatattgccg
    18961 gaagcagctt aagccaggaa gagcttcttt cagaggtact cgaaaaaaaa ctgggacaaa
    19021 gtgtctatcc cctcgcagca gaaagattac gggccctatt caagcatgat cttattagag
    19081 aaaaccttcc ggatattgtt gtgctggcag gaatagaacg tgatttgcct gaatcactcc
    19141 gacctctctc cgctaaagac ctccgcactc cttcaactct ggaagaagct ctaaaaaagt
    19201 tatgtctaag ccctgccctt cagaacacgg ctgtcctcgt tgatcggatt ttcaaggcaa
    19261 acatgcttaa ctatctgcgt gcccgtatta acaggtcagg ctctatgagt caacctgagc
    19321 tcacatcagc acaatgccct atctttttca tccagggagt aaatgcgaat aaggattcaa
    19381 cgtctgaaag gattgagcag gcagcacaaa ggcttaaaga atacagagat cttcttcata
    19441 aaaaacgtgt gcgattcata tttcttccta ttccaaacaa ggaaaccata ttgtatcaat
    19501 accttggaac acaacaacct ttatttcttg aacaattgat caaaaaatct cagaacctgg
    19561 gtattgaggt cattgatacc cagaaaacat tccatgactt ctatcaggcg acacacactt
    19621 ttccctacca tgcggacgat agccactgga atgggctggg ggtgaagata gctgctgact
    19681 tgctcgaaaa acagataatg acaaaacctc tgtctatttt ccgctgatct tcattccctt
    19741 tataaaaaca gatggcgaac cacaggtacc gtaaaatttc aggtcgtccc caacaccctg
    19801 aacattgttc agaatatcaa atatgtttcc ggaaaaaatc acccctttaa aagggccttt
    19861 ctttccaccc cggacaagat gtcccacggt gccaagagaa aaatcacctg ttatggggtt
    19921 agcagtatgg gtgcccatga gttcctcgat gacaacccca tcagtcatca tgtcacagac
    19981 attatcggaa ccaccctcaa gataaaatcc tctgcgtccg cacaatggtg gttcttttag
    20041 gccggtccga gctgcattac ccgtggagga tttgttaagt ttctttccat aatatgtatc
    20101 atagaggaaa gtcttaaaaa cccctttttc aacaagtaca ttctcctgtg aaggaacccc
    20161 ctcaccgtcg aagggagaag cacccatccc aatcaatccc gaatccacaa gagtgacatt
    20221 ccccgagaaa catctttctc caattttgtc tttcagtttt gttttatttt tgtaaaggct
    20281 ctctgcgagg aaagatcctg acaacacgct gagaatttca caagaggcct ggggcgtaag
    20341 aatgccgtca taaatacctg ttgcaatctg ttcactgtcc aaaaatgaga tcgttttctg
    20401 cgcaacttcg aatccaaggg cattcatatc tatatctgca aaggagtgag accatttcca
    20461 gtcataccat gacacttcat catagtcttt tgcaacgcag agcgcaaaaa ccgagtaaag
    20521 ggtttttctg tttctcacac taaggccgtt tgaattgaaa atttctatct ctattgcggt
    20581 ttcctgaagc tcacagttcc ttgtcgtgac aatacgttta tcataatcaa ggatcaggga
    20641 ctccatttca atgagtcttg caattttttc gctatcatct attgccagac cgtcaggatc
    20701 aaagagatcg actgatgaat aatttgtata gggagaaggc aaaacagcac tcgcatcaat
    20761 ctcgacaaaa gggctgagga gctttgtatt ttcaatcagt aaatcagctg cctttttccc
    20821 tttctgaaat gtgtaggtaa agatcatctt tccctgttta atgcctctca gagaaatacc
    20881 ctcctcttca tgtatttcaa ctccatgaag acccctgtcc ctactttcaa atttttttgt
    20941 cttctctttt aggaagaaaa gctcgtaact gtcaaattca tctcttatac gatttaagat
    21001 atggtctttc tccatccctt cccctctctc aacacctacc atataatgtt tcaccacaca
    21061 tggttagatg cgcctcatca ttgcaatttc atcacattca ggaaattttg ggcaacgaat
    21121 gcaatccgac catatttttt gtggcaattc ttttttgtca acaagtgtga atccacactt
    21181 cccaaaaaac tcttcctggt aggtgagaag aaacactttg gctatgttga gctctcttgc
    21241 ctcttccata catgcttcaa caagcatccg gcctatgcct tttccccttg atgactctgt
    21301 gacgcaaagg gatcttatct ctgcaagatc ttcccagcat atatggagag caccggcacc
    21361 aatgactttt cctcgctcct catatacaaa ataatctctc atattgtcat agagctctcc
    21421 aagagacctc gccagcatct ctcctttggc ggctgccgaa ttaacaatac tgtggatact
    21481 ttttatgtct ttaatggatg cctttcgtag catggcgaat catctcctct gccctctgga
    21541 ttgttttttc cgttattgtt gccccgccca tcatccgggc gacttcctgg actctctcca
    21601 aattatcaat ctgttttatc tctgtccttg ctgatccttt tacaaaaacc ttttctacaa
    21661 ggaaatgtct gtcagcgtaa gtagcaattt ggggaaggtg ggttatgcat attacctgat
    21721 gtttcgaggc taaatcctga agccttttgc ccacgaggtc cgcaaccctt ccaccaattc
    21781 cagcatctac ctcatcaaag ataagagttt ttccttcatc tccccctata accctcttaa
    21841 ttgccagcat aattcgtgac agttccccac ctgaggctat ttttctcaat ggcttgagag
    21901 gttcccctgg gttggtgcta atcaaaaaat cgatgtcatc catcccgttc tcatcgattg
    21961 tacccttgtt gctaatttcc atgtggaatt gcatcccttt catagacaaa aaaccaagtt
    22021 cattaacaat ggctttttca acggattttg tccctttttt tctctcttcg ctaagccgtt
    22081 gagctaaatg ctgtacctcc tgagcgagag aagttctctt tttctcaagg ccatcaatgt
    22141 ctgtggtcag ggtcttcagg taagcaagcc tttccctggc cgatgctgca tattttttta
    22201 tttcaccata cgacttaccg tatttgcttt ttaattcata gattctttgc agcctttcat
    22261 cgagtctctg aagctcgcca ggatcaaaaa caatcccttt ctctagttct ctgatttgta
    22321 aagagatatc ttccatatca taggatatcg tctcaatcct ctccatcagt ccctgtagtg
    22381 tatcgatgtc acggaagggt ttgaggaggc cagtaaatct ctttataact ccatgaacag
    22441 actcttcccc ttcatagagg ccttcatata ttgcattgag gctagcctgt atcttttccg
    22501 cttccttaat gattttcaga ttttctttca cacgctcttc ttcgttttcc tggagagctg
    22561 cctgatcgat ttcatcaatc tggaaatcca acaaagccat ctctttttcc tgcccttcaa
    22621 tctccctttt ttttgctgaa agctctgatt cacatcttcg taaggtctga actttttccg
    22681 aaagggtagt cctttcattg tcaagatata aaaggctgtc gaggatactg atataatttt
    22741 cccgatcgag gaggtattgg aactcattct ggccgtaaat gttaataagt gtatctccca
    22801 gctcagacag cctggttgtt gtgatgggct caccattgag aaaagacctt gatcttccat
    22861 gcgtgctaac aatgcgcttt aaaataaatt cctggtcttt ataaaaaaaa tgaccaagaa
    22921 tctccgcctg gtcagccttt gtcctgataa tatcgctcga tacttttgtt ctcatcagtg
    22981 ttgaaagcgc attaatgata atagattttc ctgccccggt ttctccggtt atgacgttaa
    23041 aaccgtcact gaattctatc tggagttcat cgatgatcgc aaaccctttt accttgagga
    23101 aggtaagcat tattaccttt caccccattt cagctttgtt ctcagaatct cgaaataacc
    23161 cttggacgaa gatttgacaa ggcttacaac atgggaagat tcctttatta acatctcatc
    23221 tccaaactcc agtggaaacc ctatttgccc atcgagggta agaaccactt tttcatcctt
    23281 tgactttagt atagccttaa tattcacttt ttcggggaga atgatgggcc tgttcgtgag
    23341 catatggggg catatgggtg taacaatgat gtttttcatt gaaggataca ggattggtcc
    23401 tcctgctgcc agtgaatacc ccgttgatcc ggtgggcgtt gagaatatga gaccatcaga
    23461 cttgaatgtc gtgaggtatt ctccatttac gtgagtctca atgtctataa ttctcgccaa
    23521 ggcatccttt gttatgactg catcgttcag tacggcaaat tcaaaaactt tctccttttc
    23581 tcttttgact gtcacggaga gcataatgcg ctgggagata tcataatcac cactaataac
    23641 cctttcgagc atctctggaa actcttctgg tgatatctcg gtcaggaaac ctaggccacc
    23701 taaattaacc ccaagaattg gcacgtcttt gcctttaaga ttccgtgcaa cacttaaaag
    23761 ggtcccatca ccgccgagaa ctacgataag cttcgccccc tcccctacgt gctcaatctc
    23821 aagctgtttc gtatagttga gttggcgggc tgactcttcg tcaacaaata cgtcaatttt
    23881 gtggccataa agctgaatga tacgggcagc taggctgatt gcatcttctt tctgtctttt
    23941 gcatataacg tgaatattat tcatgcacga ccctcacagg tatttctctt ttaaatggct
    24001 tgatacagac gtgagaagtt taaaggattt ctcgaccgta gtcgttttat ccgcattcag
    24061 aaggttacat gtcgcaggcg caagaaggct gttctgaatg ataagttccc tgtctatccc
    24121 tttcctctcc aggaccgccc acatcgactc aagcctgcca gcaagacccg atgaatcttc
    24181 tttcgaaaat tcttcaaaat aggtcggcac aatcccccaa ctaataatgt taccctttcc
    24241 aagaaaatct tttaccctat cataggttac aaagatttca ccaaatgcat aggcattgaa
    24301 agacaggatt tcaatgttca gggagagcag gaaatcccaa tccggtctac cgcagagatg
    24361 tagcccacgt ggcccttcaa caccatcaaa gaagaccgtg agctcctctt ttgccttcac
    24421 attatcgtag ccgcacatgg cgttaaaaac aaactcaaga ccagggtcat ctatccacac
    24481 gaaagccttt tcattctttg cacgtaattg ggcatactgt gcattgagtt ttttttggat
    24541 aatagtgtac gcaaaagcac gcacttcatc attataagct ataggctttc cattctcatc
    24601 tactattttc agcataaggc tgatagggcc gatgatttga ccgcggatgg ccttaaacga
    24661 atcaagctca agcgacagaa atcgctgata tacatgcgag aatctctctg agagaataaa
    24721 ggtagtaggg tctttttcga gttcgatgta caagggaatg tcatttataa acctattcgt
    24781 atcaaaatat atctgttgtc gatcctcatc gattaccatg ccgggaaaat attccattgc
    24841 ctggacgtac atgtcctcag agaaagagag tttcggcaac tggggccaga acggaatgtc
    24901 aagggagagg gcaagctcca gagcatcatc tatattttca tggggcaaga ttcccatagc
    24961 ggttgttgac aaatttccac aaaatcccag cctcctgtcc atatagattt cctaacattc
    25021 aggacgttac ttgtcaattg cagttcgttg ataattaatt ggatttttct acaacacccg
    25081 caccacctgt atctcttttc gattgacatc aacgtattgc ccatagacaa agtctaccca
    25141 atcagcaagg gggattttaa gcagcaattc cgatgaaagg attattcccg caaaaaatct
    25201 ttgaatcata ggtatcagca tgaggaagtc tggactttag ttatccaagc tggtcagtat
    25261 cactgacatg ttttggggca ggagtcacaa gaattttatc gattcggtta ccatccatat
    25321 cgatgacttc aaatcgtggg ccggcaaagt caaaatgatc ccctgttgaa ggaatacgat
    25381 taaaagaaaa caacctgaaa cccgcaatcg tgtggtaacg gcctgaatct tcatggggca
    25441 tggcgccaat ttgaaaaacc tctttaaact tgtcaatata gactggcggc catcaaccaa
    25501 ccaggatcca tatgccctgg gaacaacatt tgagtcctct gttaattcat ctgatggcaa
    25561 atcaccaact atagcttcaa gaatgtcatg gagagtaact ataccctgaa ttgctccata
    25621 ctcatcaacg atcaatgcca catgcgttcg tgactgttta aagagttcca gtatcttaag
    25681 ggcttttaaa gattctggtg caaacaaagg ctgctgaata atcccctgga tgcccttgac
    25741 ctgatcatca ctgggctgtc tccaaatatc cttgatggaa actatgccca aaatattatc
    25801 agggtcttgc ctgtaaacag gagactggga atatccgcac gtttttattt ttttcgagat
    25861 ttcctcccaa ctatcatcga catcaatgga aatgatatcg ggtcttggcg ccatgatgtc
    25921 gtttacattg cggttaccga ggcgtagaag atacctatac aggaacaacg ttctttctca
    25981 acaaatgtgg gattatgctc ctagaaattc ctgaaaggaa cctcccagaa gaaacagtat
    26041 tgaacccaga aagaagtgta cagatagaat taacaaacca tgaaatcctg aatgtgaacc
    26101 tattcatgga aatgccacaa gaaagatcgc gggtaagcgt ttatttcaac ttctcccccg
    26161 agtctatcta tctttgtgga aaagaaaagg attttattct gaacaagtca ttcgtatttt
    26221 cggtgaggga tctggatctt taatagacct actatccatg ccacaattta tcttggttaa
    26281 accacacatc ctcaaatatc aaccatcctc agatttgttt tactctatgt tgctacgtga
    26341 aatggaaggc tgggtgaaag ggaagcatac aaatacattg ttttgtctgg tacaaagccc
    26401 atcagtgttc ctcagagaaa taatttggaa gatggaaaac ctgttggtca tcaaagaaaa
    26461 acccatatct attggtattc ttaaaaacca gacaaagggc atcgcacata gttataacat
    26521 tttttgatag aatccacatc tttggatatc tttcccgcca tccaaacaca aaggcggtta
    26581 gctatacggt ctccagaaaa aagccttttt tgggcagtgt tggaaatttt atcaacacca
    26641 caggtgacaa ctcgggttgc caatgaaaaa aggaagggac taatccgatt cactaaaagc
    26701 tatttagaca ttcctacttt ttcctgcaca tttattaaaa gaatttaact tgactttgaa
    26761 tctcggcctg gggtatttta cacacttatg cagttaggat cagagtttaa aatattatct
    26821 atacttgtca caacaatcat tgcaataact ttcattgcac tgacctctgt ttctgcttca
    26881 gggggtgaag gtgggcacga aaacattatc accaacgaga tcattcagta cctcaaaaat
    26941 aagaaagtca gggcaagtga tgaaaagcta aagaaaatag ctatgtctgt atatgaagaa
    27001 gcaaaagata atgaaattga ctatcggctc gtgctggcag tcatgaaggt tgaaagcaat
    27061 ttcaggagtg atgctatttc aaaaaaggga gcccgtggac ttcttcagat aaaaccttcc
    27121 cttgccaaat atgtatcaaa agaaatgggt gtttcaataa agagtcctaa atgcctggac
    27181 gaacctgaaa agaatatcag aatcggagta agccaccttt cgtggctcat cgagaagttt
    27241 gagaatctca acgcagccct tcatgcttac aacgttggca taggaaaagc caaaaaagtt
    27301 gcgtcagagg atgatggccc ggacacgcac ttcaccagga aagtcctggc tgaatatcgc
    27361 ctgattaaaa acgcactgcc acattcccat cccgaacagt gacatataaa taggaaatga
    27421 aagaggtaag tggggttgga aacatgtcgt tttcaacaca ataaacgaat aactcgatca
    27481 acttagtgtt caattaacca atcacctgat aacaaaaaat gtcttgcatt gatgctgttt
    27541 atcctgtatt cttccacaac acgcaaagac actatgccaa agcagaaaca ctttcaggaa
    27601 cccctctttc aggaactcaa aaaagactac tgttacgtct gctatgaacg catcaaaaaa
    27661 gatgaaggca tttatattgg caatggtatg tggcgtcaca aaaaatgtaa gcccggcagc
    27721 aacaggtggc taaaaagcag ggtcggtcag gagaatccac ttttatcctc ctgcgaccag
    27781 tcgacgaagt aattctctac ctacacaact acttgagcac atttttgaca tacgctatac
    27841 cgagcgctcc tggaccagca tgagatgtta tgacccctcc aagtttaccc cggaacatga
    27901 gcgaaatacc agggattctc tcaagcctgc cgaggagatc tgtcatgacc tctgcattgt
    27961 ctgactcccc taccgccatc aatagctgtg aatgttcttg tactgccagt tcaaccagct
    28021 ctacaaacct gttcaacagc ctttcactgg tacggatttt ttctatgggt tggacttcac
    28081 cattcattac tttcaaaagg ggctttatct tcatcaaagt ccctaaaaaa acctgggctt
    28141 taccgatcct acctccacgg tggagatact ccaaagagtc tactgagaag tacgtctcga
    28201 tctgtgggcc aatctccctg gccacatgag caacttcgct cagcgaagcc cctctgttcg
    28261 ccgtttcagc acaaataata accagtatac ttagggcaaa ggatgcctgt aaagagtcta
    28321 ccacttctat cattgccccg gggaaatggc ttcgcgctaa cagagcagac tgaaaaacgc
    28381 cgctcaattt tgatgacaga tggatcgaca gaatcgattc tcctttttca acaaaggggg
    28441 aataagcatc aataaaatgc tggggtgttg gttgagatgt cgtagggtgt gctgttgttt
    28501 ttctcaggag cctgtaatat tcatctggtt caacatcata ataatccttc cagcttctat
    28561 ctccaagatg gatggttaac gggacaatcc caatattatg cttaatgatc aactctttcg
    28621 gcaggtcagc agtgctgtct gttatgattt tcatatctac tgctccacac tactcaactg
    28681 aaacgatata ttgtgcatgt gcttgaccac caaaatataa ttcaacactc ttgtcgggga
    28741 aactctgctg aagaatcgac tgcatagctt ctgcatcatt ttttctgatg atttcaccaa
    28801 agtaaagtgt aataatctca tctgacggct cgtacatgga cttaacaaga acctgtaatg
    28861 tttcacaaag ggacgcagaa gaacattgga tttctccccg gtaaacacca atgaaatcgt
    28921 ttgttttaac atcaacgccc cctaatgtta catcccttga tgccaccaga atttctccgg
    28981 attttacctg ccgggatgcc atatccattc gactgacatt ttcctcgaag ggtgcatcat
    29041 ccatataagc aagtaatgca gaaattcctt cagacggtgt tgatgttttg aggacagtga
    29101 cttgttttgt tgcaatctct gccgccttga gggcagccgg atagatgttt ttgtggttgg
    29161 gaagtataac cacattcaat gattcaactg cctctatggc agaaactata tccatcgtac
    29221 ttggattttc tgttgccccg ccatccacaa caagctcagc accggcattg taaaagattt
    29281 ccttaaatcc atctccagga acaatggcaa caacagagct tgtctttgca cccttgtggt
    29341 tgacaaaacg cgacgtatgc tgagtgagca tatcatccac cttgatcgat gaaacctgcc
    29401 ccaatgaaaa agcatactct agtacctttt gaggctggcc tgtatggatg tgaaccctcg
    29461 caaggcggct atctcctata accacaattg aatcaccctt ctgggaaagg tcttttttca
    29521 ggtgatcttc tgagatctga cttcctttga gaataaattc cgtgcaataa cgattgctgg
    29581 gttgagaggt tgaagtttct ttctgcatat cgaccaacat cttttcatta tcaacagttg
    29641 tatcatccat tgctatgccg tcgacaagac gcagcatacc ctcgagaaag tagaaaaacc
    29701 ccaacccgcc tgcatcaatc acccctgctt cttttaattt gggcagcatt tcaggagttc
    29761 gctcaactga ggtccgagct gctgttacca ttgcatgaag gagaaaataa agatcagaag
    29821 tattctcggc tgcctttatt gctgcttcag ctgtctctcg aaccactgtt aaaatggtgc
    29881 cctcagttgg ataaagaatt gctttgtaag cttttttaac agcatgggag aatgaaaggg
    29941 ctacatcctt tgctgacagt ctttctttgc cttctatccc ctcagctatg cccgatagaa
    30001 tctgggcaaa tattatacct gaattacccc gtgcccccat aagggctccc cacgcagttg
    30061 ccttcactat cgtttcgaga gacggatcct gcagagcttc aatctctctc acaactgaca
    30121 taagcgacat agacaagttg cttcctgtat ccccatctgg tacagggaaa acattaatct
    30181 tattcaggtg atcccggttc ttaaaaacag actcagcccc tgctaaaata cataacttga
    30241 aacgttttgc acttagatat gctattgtct tttttttctc gttcggtttc atgccataaa
    30301 ccacctattt tcattcagcg tgagaatgat gaaggcaggc attcaaggat aaacaaaaaa
    30361 aactggcaag agtatagtat attttccatg ttggtaaaac gttttaatct cttctgtcaa
    30421 accaaaatag aaatgtccct tgcttaccaa agtaaaaatg tccggtatgg gcgcattgat
    30481 gttttcgttg attcctggaa tgaacccgta tgtcttgtca tactatattc acctcatctt
    30541 tgtgctgtaa aaatgtggta ggcggatgtc ggatatacat acccctttag tggcgatact
    30601 aaaagcacaa aactgtggcg aatccgggag acatgcgtca taacagacat tggggcgtat
    30661 acacaggtta ggcaaatgtc atcaccgttg aactggaatg ggttttttaa ttacttttgt
    30721 attccatccg atgaaataat agaatgtttt ttattagaat aatatccata tgacaggcga
    30781 tttaaaaata aagatacaag aaaccatcga ttttctcccc cccattcctg cagttatggc
    30841 agaactttta gtggccctca acgacaagga tatcgaactt gaggcgcttt cccatataat
    30901 ctccaaggac cccttgatga gcatgaatgt tcttaaaatc tcaaattcag ctttctacag
    30961 gttacccaac aaagttgaga cgatagatcg tgcagtaaca ttgttgggca ttaaagagat
    31021 taccgggata tgcattgcat gcggtaccta ccaggcgctg aagccttcca aaaatgtgga
    31081 aacgttcgat ctccatgact tttggagaca ttctgtaacg accggtgttg ttgcaagaag
    31141 gttatcagaa gaactcggga tactggacca caatgttcta tattttcttg ggctcctcca
    31201 tgatgtagga aaggtcatac tcgacagagt tgctcatgaa acctataaag tggttatcca
    31261 ggaaacactt caggaaaata tcaccatacg agaagtagaa aaacgcctaa ttggtgagtc
    31321 tcatgatgta gttggtgcat ggcttatgga gaaatggaga ctacccataa cgtttgtcga
    31381 tgttgccaag tatcatcact ctgtgaatga aacaccggag gacaacaggg ctgcaacagc
    31441 aatcatctcc tttgcagaca gattgtcatg gatacattat gcaggtttgg aatccaattt
    31501 cgatgtcacc cttttgaccg aacatggtgg ttatttattt cttgaagaag taaccgatat
    31561 catcgacgaa gttgatcttg gaaagttcat gcgagaactc gattcggcaa acgacgagat
    31621 tgatgaaatg atgaaaatcc ttcagttgtg atttggctgt ttaaccccca ttcgtttaac
    31681 caaattaatc tgggcctcag gttttcacaa gattttcaag aatagcggca atttctgaaa
    31741 gcacaacagc agttgcaccc cacaccttgt ggccatggac attaaaataa gggacttgct
    31801 taatctcgtg gtcgaggggc cattcttcaa tgacaacatt tctttcatcg agaaggtagc
    31861 gcagaggcac ttcaataatt tccgccactt cttcgggttg tggaagaaac tgaggcgatt
    31921 gaaacatgca ggccacgtat ggctgaacac agaagtcact tggcggtata tagatggggg
    31981 tcaaggggcc aaggatacgc atagtgtcaa tgcgtacccc aacctcctcg catgcttctc
    32041 tcaacgcagt gtcagcaatg ctgtggtcat ggggatcaac ggctcccccg ggaaggctta
    32101 tctgaccctt atggtctgcc acgttttctg tgcgacgggt cagtacaaag cagagttcac
    32161 ctgtttccac agatgggaaa agaaggacca gcacaccgct ttttcttggt ttggattcgg
    32221 gtgccgggga cttcagtgag cctggtcgag gagccatacg gagttgcgct tttacaccgg
    32281 gcaaaggctt ctgaagttgg gcctcaatca tgtcaatagt aaattcaaac atggaacatt
    32341 tgtcagttcg ctacgaatat tttgccggga ttcagaatgt aatttgggtc aaatatcctt
    32401 ttgatgcttt tcattgtctc aatcacaaca ggagaaagct ccatgccaat atacggtgcc
    32461 tttgcagtcc cgataccgtg ttctcctgaa agcgtaccgc cgagacgaat ggtttctgaa
    32521 aaaatctcct ctaccgcctt atgagccttt tcataatctt ctttcgtatc ctttatcata
    32581 agactaacgt gaaagttgcc gtctccagca tgaccaaagc tcaggattgg gataccatgt
    32641 ttcttgcctg tttcctccag aaagcggatt aaggttggaa tattcgatcg aggcaccact
    32701 acatcctctg caatcttcac aggattcagg ttataggtag cctgtgaaac cactcttcgt
    32761 gcctgccata gcttctctgc ctcaaccgga tcctgggtca gttttacatc agtagcatta
    32821 tgctgtacaa gaactgtctt gattttttgc gctaacgttt taatagcatc gccatcacca
    32881 tctatctcaa tgaggagaag accttctata tcctcaggaa tacccattgg atttgcctgt
    32941 tcgctgcatc ttatggaagc ctggtccata aattcgattg tggagggaac aatcctggca
    33001 gccacaatac cggatactgc ctctgccgta tcttcaacat gactaaaaaa ggcaagaata
    33061 gttgcttttg cttctggggc ggg
//
LOCUS       DAOOMC010000012        33078 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdus sp. isolate AMR_MDS_2685
            NODE_1112_length_33078_cov_6.331496, whole genome shotgun sequence.
ACCESSION   DAOOMC010000012 DAOOMC010000000
VERSION     DAOOMC010000012.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864760
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdus sp. (wastewater metagenome)
  ORGANISM  Syntrophorhabdus sp.
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae; Syntrophorhabdus.
REFERENCE   1  (bases 1 to 33078)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 33078)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,729
            Genes (coding)                    :: 2,708
            CDSs (with protein)               :: 2,708
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 7 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33078
                     /organism="Syntrophorhabdus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1112_length_33078_cov_6.331496"
                     /isolate="AMR_MDS_2685"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2699756"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1851)
                     /gene="uvrB"
                     /locus_tag="PK125_02170"
     CDS             complement(<1..1851)
                     /gene="uvrB"
                     /locus_tag="PK125_02170"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: protein motif:HMM:NF003673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HPB36947.1"
                     /translation="MPNFKLISDYAPKGDQPQAIEKIAKNLVDGVPHQVLLGVTGSGK
                     TFTMANVIERVQRPTLVISHNKTLAAQLYSEFKTLFPENEVHFFVSYYDYYQPEAYKP
                     QTDTYIEKDSSINEEIDKLRLLATNALFEHDDVIIVASVSCIYGLGSPEAYYGMLIVL
                     EQGQNMTRKMLLDKLIDCQYERNDTDFYRGKFRIRGENIDIFPAYEEENAYRIIIDDD
                     RVESIHTIDPLTGKTINRLRRCTIFPATHYVTPRHTIERAILTIEEELGERLFLLRSQ
                     NKLLEAQRLEMRTKYDLEMIREIGYCSGIENYSRHLTGRLPGEPPPTLIDYLPENAVI
                     MIDESHVTIPQLIGMFRGDRSRKTTLVEYGFRLPSALDNRPLTFEEFEEREGQVLYIS
                     ATPASYELKKAKAHVVEQIIRPTGLMDPKIEVKPAKNQIDALLPEIRAVIEGGERILI
                     TTLTKRFAEDLTDFLLDGGIKAKYLHSDIDTLERVAIVRDLRKGKFDVLVGVNLLREG
                     LDLPEVSLVAILDADKEGFLRSQTSLIQTCGRTARNINGRVLMFGDIVTDSMRRAIEE
                     TERRRKVQAQYNKEHGITPEGIKKGVVDILSSIYEKDYYTVPVDELEEQGI"
     gene            2439..2999
                     /locus_tag="PK125_02175"
     CDS             2439..2999
                     /locus_tag="PK125_02175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB36948.1"
                     /translation="MKSLSVKIILAFALIIGFAYGNAYAQGVDFSIWDGSYWSTKFTA
                     KGVLFSDPATPPNKKINGTEKSWAIMISSADGSEMTLQFYQKNDRGECVKGDEIPITL
                     MYGGPLGFVATITIDEPEPDFNSARVLLYFTGKLKGEALTSGKIATLGGYAIEQDWDD
                     VTDRAAFGVTLSGTLLKEPKGCLLDK"
     gene            complement(3090..3713)
                     /locus_tag="PK125_02180"
     CDS             complement(3090..3713)
                     /locus_tag="PK125_02180"
                     /inference="COORDINATES: protein motif:HMM:NF014215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HPB36949.1"
                     /translation="MSIKRVIIEGACKLEGIINENSRRGGVVICHPHPLYGGDMHNNV
                     VGAIEKGFAYKGFTTLRFNFRGVGESTGFYGNGQGEEEDLLDAAEFLMEQIDKDGLIM
                     FAGYSFGAWICSKAAPKLERLDGLFLVSYPFAFYESESLKRYKGTIFLVGGKSDDIGP
                     IDRLIETYKAIPSLQKSLKIVPTDHFYQGNEKEITDFIKEVVPSPGI"
     gene            3768..4637
                     /gene="accD"
                     /locus_tag="PK125_02185"
     CDS             3768..4637
                     /gene="accD"
                     /locus_tag="PK125_02185"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HPB36950.1"
                     /translation="MGFFTRKEKRLKKKPHKVIDIQKEMKRAETDESLWIKCSSCEGL
                     LYKREVERNQHVCPKCFYHFPLTVENRIATIFDKGSFTELFSNIEPVDFLKFKDTKPY
                     KTRLIETKESTKKTDAMICGKGKINGFEVYTAIFDFSFMGGSLGSVVGEKVTRILEEG
                     LNQRLPVIIFCSSGGARMQEGIMSLMQMSKVSGAIYRLKSSRIPYITVLTDPTLGGVT
                     ASMGMLGDIIISEPKAMIGFAGPRVIKDTIKEELPQGFQRAEFLFEHGMIDMIIPRKE
                     LKQRLHAILSMIT"
     gene            4634..5899
                     /locus_tag="PK125_02190"
     CDS             4634..5899
                     /locus_tag="PK125_02190"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folylpolyglutamate synthase/dihydrofolate
                     synthase family protein"
                     /protein_id="HPB36951.1"
                     /translation="MTEYTNALAYLYNLEKFGMVFGLDNIRWILNLIGNPHEFIKAVH
                     IGGTNGKGSVAAMLSHVFKAAGYRVGKYTSPHLVSFAERITIDEKKVTEAEVADLVVS
                     IRQAVEAVDPRRTFTFFDFTTALAFEHFRKNHVDLALIEVGLGGRLDSTNVINPLVSV
                     ITNIAYDHMDYLGEDILRIAREKAGIIKRTVPVVTGAQDLPGQIIEETGRFNDSPVYV
                     MDRDFFYEKKGTQSMTYSGLSIKLDDVFVNLKGDHQLGNAAIALCTSELLSMLGFGMD
                     RRSIYEGLSQVTWPGRLEIIKERPEIILDGAHNVHGIKTLASFLKNQFPDRRRLLIFG
                     VMKDKEYEKMLDELLPVVDEVIFTSSSNDRALQPESMKKVAPRAVIADDAAGALRKAK
                     AMSGGNDVIIVTGSLYLIGECRAIINDIF"
     gene            5937..7985
                     /gene="lptD"
                     /locus_tag="PK125_02195"
     CDS             5937..7985
                     /gene="lptD"
                     /locus_tag="PK125_02195"
                     /inference="COORDINATES: protein motif:HMM:NF016348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0010033 - response to organic substance
                     [Evidence IEA];
                     GO_process: GO:0061024 - membrane organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly protein LptD"
                     /protein_id="HPB36952.1"
                     /translation="MPVFASGKNSAISLPKFTITTEPVEISANSLEYDKINNVYKAKG
                     AVDLQEGTRRLMADEVVYNMDSGDIAASGNIVFQDGEDIIKCERLSLNLETKTGTIEK
                     GTIYIKQNNFTVTGEQIEKVGDQQYKIKKGQFTTCDMPQPDWKFSARDVDITVEGYAK
                     TKGTRFHILDQPVLYLPYGLFPVLTKRQSGFLMPDLTVSSRDGIKIKNSYFWAIDRDK
                     DATIGLEYIEKRGFKPELEYRYDLKEGLKGAWNFAIIDDTKYDHWRYEIKGKHEQTLF
                     KDLRLKTNIDYVSDYAYLMDYRQGLDERSENHLKSTAYLEKPFSHSLLTVEGAYFRDL
                     SRKDNDNTFKFYPHATFFTDYLPLIRRVLYADISAEASNFYREEGNTYSRISIEPRLR
                     LPFSWNGVNFLLSGTLFEKFYYIDESKPKDTSTKNLGTARFDGSTNVQFMRNYNVDLW
                     NIGTVQSVIRPQVQYTFISNNSYREIPLIDPFDRIGKTNTITYSLSHYLNTFTPQVGG
                     REISLLEVFQTYGMSGNLPLSDAYRGYGNRFSDITGRLTLFMKKNISFTNEAALNVHG
                     EGITATRNVLRYIYPEVYYMNISHNYTQSLANQMDLDIGGKYKFLTGRYRIIYSFKNK
                     EWIDTQYQLIYQPQCWAVILTLKQTKRPNDTSINIGFDLTGLTGKIDDINKTFGIYQR
                     "
     gene            8013..8741
                     /locus_tag="PK125_02200"
     CDS             8013..8741
                     /locus_tag="PK125_02200"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HPB36953.1"
                     /translation="MKGVILAGGLGTRLSPLTKITNKHLLPIYNKPMIYYPIEALIKA
                     GITDIMVVTGGNHAGDFLRLMGNGKEFGLKHINYTYQEGEGGIADALSLAEYFAEGEP
                     ICVVLGDNIIEKNIIKAVKDFGGQKKGAKILLKEVPDPERFGVAEIVNGRLANIVEKP
                     RQPKSNLAVIGIYLYDSRVFDIVKTLKPSDRGELEITDVNNAYVREGTMTWEMLQGWW
                     TDAGTFESLLRANVLVAQTGANNG"
     gene            8929..9384
                     /locus_tag="PK125_02205"
     CDS             8929..9384
                     /locus_tag="PK125_02205"
                     /inference="COORDINATES: protein motif:HMM:NF013103.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase family
                     protein"
                     /protein_id="HPB36954.1"
                     /translation="MIKGVATKQLKFIPDERGRLMEILRNDDEMFIKFGQVYLTTTYP
                     GVVKAWHYHKEQDDYITCIKGMLKLVLYDSRERSATKGELNEFFIGEYNPMVVKVPAM
                     IYHGWKCISDEEAIVINVPTEVYDRQKPDEYRLDPHKNDIPYNWERKDG"
     gene            9377..10390
                     /gene="rfbB"
                     /locus_tag="PK125_02210"
     CDS             9377..10390
                     /gene="rfbB"
                     /locus_tag="PK125_02210"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007846915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HPB36955.1"
                     /translation="MDKTRILVTGGCGFIGSNFIRYMLSKYPYIIINFDKLTYAGNLE
                     NLKDVESDSRYTFVRGDIASKADLASVFEQGIDVIINFAAESHVDRSILDPDTFIRTN
                     INGTFNLLEEARKREVKRFIQISTDEVYGSLGKEGKFTESTPLSPNSPYSASKTAADM
                     LAMAYHKTYGTPVLITRCSNNYGPYQFPEKLIPLIITNALEDKELPVYGDGMNVRDWI
                     HVIDHAVAIDAVLHKGVLGNVYNIGGENERTNIEIVKLILSILGKPESLIKYVTDRPG
                     HDRRYAIDSSRIKRELTYSVTTDFRKGMEDTVKWYLDNRGWWERIKSGAYLEYYDLMY
                     KNR"
     gene            10403..11233
                     /gene="rfbD"
                     /locus_tag="PK125_02215"
     CDS             10403..11233
                     /gene="rfbD"
                     /locus_tag="PK125_02215"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: protein motif:HMM:TIGR01214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008831 - dTDP-4-dehydrorhamnose reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HPB36956.1"
                     /translation="MKILTTGSHGLLGTNILPVLERSFTVIPYDIEEWDITDRDAGER
                     TIRDIRPDVILNLAAYTNVDECEDCLSLADRVNAEAPGIIAKLCSDHNIKFVHISTDY
                     VFDGEKETPYREDDETNPTSVYGLSKLSGEKKVLANCPDATIIRTQWLYGHGGINFIT
                     KITKIAREQGVAEVVNDQRGCPTYAKDIADPILLLIEKNRSGIYHVANAGSCTWFDFA
                     REIFSCLHIDVVLKPITSEQLNRKANRPRNSVFDCSRLFRDTGHRMRTWQDALHDYLS
                     "
     gene            11240..11962
                     /locus_tag="PK125_02220"
     CDS             11240..11962
                     /locus_tag="PK125_02220"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: protein motif:HMM:NF024258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008758 - UDP-2,3-diacylglucosamine
                     hydrolase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HPB36957.1"
                     /translation="MKMIFFADTHLTKKNKKKLRMVEIFIRNVCIDADMVFVLGDLFE
                     FYHGYEGYIYPWYRPIVDAFKTITEQGKSVYFLEGNHEFDMGPFFESYTGIQCVKNLT
                     IDIEGKRTFISHGDEFYLNLVTRALKAPLTIGAMNLFGPRLSWKIAMAVSRIMSKKKK
                     GQNPKVKNVFREYAREKLKEGYDAVILAHTHIADYMEFGAGMAKKVYLNTGDFIRKST
                     YIEYTSEEGFQLKEYKPLIRGI"
     gene            complement(11949..12227)
                     /locus_tag="PK125_02225"
     CDS             complement(11949..12227)
                     /locus_tag="PK125_02225"
                     /inference="COORDINATES: protein motif:HMM:NF013500.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="barstar family protein"
                     /protein_id="HPB36958.1"
                     /translation="MSVTKYVLNGRAIQSLNDFYDEIVQALSLPEHFGRNLDALADIL
                     TTDIKGPFEIVWESSSGSKKALKKDYSKIASLLKRVAKQRDDFKVIFL"
     gene            complement(12218..12697)
                     /locus_tag="PK125_02230"
     CDS             complement(12218..12697)
                     /locus_tag="PK125_02230"
                     /inference="COORDINATES: protein motif:HMM:NF012755.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease domain-containing protein"
                     /protein_id="HPB36959.1"
                     /translation="MIGRITHVCLTVALAVFAGFLFSTTSHAVNCETVVDDLNAKLNQ
                     KIDTNELIDILRSLNTTNNRELPKKFITKKEASQAGWKPGTSLWSVDRLRGKSIGGDR
                     FGNYEKKLPNGKRKWREADLDYHGGRRGPKRIVFSPDGLRMITVDHYQTFSEVQECQ"
     gene            complement(12828..>13047)
                     /locus_tag="PK125_02235"
     CDS             complement(12828..>13047)
                     /locus_tag="PK125_02235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB36960.1"
                     /translation="TKLDRTAMVSHEFEADKSVLCATPYLKRTQSSGIWKKRGHSEQA
                     AKDVGLKGSKKARAKKKAEKPVAPAPSK"
     assembly_gap    13048..13147
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            14037..15056
                     /locus_tag="PK125_02240"
     CDS             14037..15056
                     /locus_tag="PK125_02240"
                     /inference="COORDINATES: protein motif:HMM:NF012360.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB36961.1"
                     /translation="MVILFAHSVRTAIKGGTMGKSVKGENAKGACRRDISVMYNFRDE
                     IPGIVDTIVALHDQDDRFCHISPEPPPSRMLVNEIIRRGMRILYPGYFSETCMDEVNM
                     RYYLGQEIIEFYQLLSEQIIAAIRHDCVRYDLVCTNCDERGQRIALQFLKDISNLQAV
                     LAKDIIAAHEGDPASKYYDEIIFSYPGLFAITVYRMAHNLLEQGVPLIPRIMTEYAHN
                     ITGIDIHPGVHIGESFFIDHGTGVVIGETTTIGDRVRIYQGVTLGALSLSKEEVERLR
                     NKKRHPTIEDDVIIYSNATILGGNTVIGARSVIGGNVWITESVPPDTTVFLKKPELVI
                     TGKAK"
     gene            15164..15376
                     /locus_tag="PK125_02245"
     CDS             15164..15376
                     /locus_tag="PK125_02245"
                     /inference="COORDINATES: protein motif:HMM:NF014304.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional coactivator p15/PC4 family
                     protein"
                     /protein_id="HPB36962.1"
                     /translation="MEIGELQKGKDKIIIALKEFKGKEYIDIRTHFENSDGDWIPTKK
                     GVTFSPENLDEMIELLMAAKKKMSER"
     gene            complement(15550..16116)
                     /locus_tag="PK125_02250"
     CDS             complement(15550..16116)
                     /locus_tag="PK125_02250"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HPB36963.1"
                     /translation="MSKKIAVFVGSLRKESYNRKMAKALASHAPETLELEIIDIRGLP
                     LYDQDLDDEGKPPASWTDFRKRVKPFDGVLFVTPEYNRSTPAVIKNAIDVGSRPYGKS
                     VWDGKPGAVISVSPGAIGGFGANHHLRQSLVFLNIPTMQQPEAYIGNAANLFDERGNL
                     ANDSIEEFVTKFMQAFARWVDIHTSRQG"
     gene            complement(16190..16657)
                     /locus_tag="PK125_02255"
     CDS             complement(16190..16657)
                     /locus_tag="PK125_02255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB36964.1"
                     /translation="MKKGLNRINMEIKIKKIDCHQVDGSASDMISTYNVMQNNREFRI
                     TCRSNIFGRSFSLDGMDGTLYTNREDNMVHVQKVALGGGCGLIIDDEPIEGLSPLVIR
                     AIILAEQNTETREITITIKRLEDGACHPVLLIDGTRKNIYCFSDIFPDIEGLR"
     gene            16930..18132
                     /locus_tag="PK125_02260"
     CDS             16930..18132
                     /locus_tag="PK125_02260"
                     /inference="COORDINATES: protein motif:HMM:NF012766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HPB36965.1"
                     /translation="MLQKVGVFEPASKDELLGRIENLRQLMENAGIDYALIIQNVDCF
                     YFSGTMQKGMLVIPLDQDPMIFVEKGAERAAMETPLELTPIKNDKEIKKILSDKHILK
                     GMAGLEFDVIPVSVFERLKRVVGFDRCKDVSELIKETRIIKSSFELAQVRKSGEMLSR
                     VFEKAHNVVQEGKTELEIEAALVAEGRRIGHQGFLRMRGLNQEMMVSTVQAGYTGTIS
                     TMVDGAITGTGVTPAMPQGSSLKKVERGVPVTIDYGGAYNGYITDETRSFVVGELQDV
                     FRRPYETARSIIEDVMTYGRAGINCTEIFYRAYEIVKKEGLEEYFLGHGDGKVVFIGH
                     GLGLEINELPVITARHSRILQEGMVFAFEPKFVLPPYGAIGIEVDFIVRADCLERVTA
                     NSIDIVTL"
     gene            18261..18830
                     /locus_tag="PK125_02265"
     CDS             18261..18830
                     /locus_tag="PK125_02265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB36966.1"
                     /translation="MRRYGKSGVNADSMVKTEKDMKGRRLSWMNKTIRKQRNSKGKDK
                     IILKDSLIGLYNEECFNEFLDLEKRRCKRSEDPAFLMLADLSAFVNESERHDVVKSMM
                     EVFCEATRDTDVKGWHIDGLVMGILFTEITGDEATSRVALKHIASKCLWRLKYTLGAD
                     KFSNIRITWQSLKSEHMLEIHKVGNVGSD"
     gene            19032..19904
                     /locus_tag="PK125_02270"
     CDS             19032..19904
                     /locus_tag="PK125_02270"
                     /inference="COORDINATES: protein motif:HMM:NF014615.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HPB36967.1"
                     /translation="MKLSEFLTSIERTMLLDGAMGTQLAEAGLDMGGKTNITHPDAVL
                     SIHKQYAECGVDMIITNTLTMNRINIELHNMGVDVAEVNSAGVRLARAAICEGQYVLG
                     DMGSTGKMLKPHGDLSPDDAYAAFKEQAIILAEGGVDGIIVETMFDLAEAICCTRACK
                     DAVDLPVIVSISFSTMKRGGRTIMGNSAQDCAKALVEAGACVVGANCGNIDPFQMSEI
                     VSTMGEVVSLPLLAQPNAGIPRFVEGRTVFDMSPSDFAEGINRCIERGARLVGGCCGT
                     SPDHIRAIVERIRG"
     gene            19999..20532
                     /locus_tag="PK125_02275"
     CDS             19999..20532
                     /locus_tag="PK125_02275"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: protein motif:HMM:NF015048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HPB36968.1"
                     /translation="MEKSVNTSEMEENPMDGKRVSESAVHTAQLMSPQDANAAGNVHG
                     GVIMKLIDDAGGAVAYRHARSNVVTVSIDRLNFLNPIFVGDLVTFKASINMAGKSSMD
                     IGVRVEAENLITGQVRHAASAYLTYVALDVDGKPKTVAPVILETAEEERRFRQAHARK
                     EIRRQAQESHARLAPEK"
     gene            complement(20564..21289)
                     /gene="thyX"
                     /locus_tag="PK125_02280"
     CDS             complement(20564..21289)
                     /gene="thyX"
                     /locus_tag="PK125_02280"
                     /EC_number="2.1.1.148"
                     /inference="COORDINATES: protein motif:HMM:TIGR02170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050797 - thymidylate synthase (FAD)
                     activity [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent thymidylate synthase"
                     /protein_id="HPB36969.1"
                     /translation="MEAQLKVMLVSYSPEPEKTISAAARLCYSPADAEDILSKVGDND
                     QQDFLGKLTAVGHLSPVEHASFTFVVEGISRACSHQLVRHRLASYSQQSQRYVNEREF
                     DYIMPPSIKENGEMKAHFEKMMEEMQQSYSYFADKLAQLGHSTESAREDARFVLPNAT
                     ETKIMITMNARELLHFFRQRLCNRAQWEIRAMALAMLKLVKPVAPTIFRYSGPPCVKG
                     KCTEGPRTCGKSEAVRAFFLQEL"
     gene            complement(21332..21703)
                     /locus_tag="PK125_02285"
     CDS             complement(21332..21703)
                     /locus_tag="PK125_02285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB36970.1"
                     /translation="MAKVNLKRYGLYLIRWQLSTPILAGVLYVLSYMDKITATVIANL
                     IGGLIFFWVDMFIFTSDRLSVQWEIRDVVVCVDCGKEARGYRIIKAKEYDKTKDAHPE
                     FRCEACSKKKTEELRRRGIEI"
     gene            complement(21802..22470)
                     /gene="thiE"
                     /locus_tag="PK125_02290"
     CDS             complement(21802..22470)
                     /gene="thiE"
                     /locus_tag="PK125_02290"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HPB36971.1"
                     /translation="MRKPSLQDYRLYFVTDPILHKGYSVLEQVELALRGGVKIIQIRE
                     KNLPLPEYSKLASEAMKLTRTHNAFLIINDAVDVVKEVAADGLHLGQEDMPITKARAI
                     LGNDVIIGISVKTVGEAQQAERDGADYLAVNGVFPTQTKEDLGYCPGLEGVAMIRNNT
                     VLPIVGIGGINLNNCRSVLQAGANGIAVVTSITMSEDIPGTCRSFFKLMSTVDSEKME
                     RDRD"
     gene            complement(22492..23313)
                     /gene="thiM"
                     /locus_tag="PK125_02295"
     CDS             complement(22492..23313)
                     /gene="thiM"
                     /locus_tag="PK125_02295"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: protein motif:HMM:NF006830.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004417 - hydroxyethylthiazole kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HPB36972.1"
                     /translation="MTEKELSKKTIEILLEIRKHQPLVHHITNFVVMNSTANVTLAIG
                     ASPIMAHAHEEMEAMSAFTSVLNLNIGTLTPYWLDSMILAGKVAGERGIPIVLDPVGS
                     GATPLRTEAARRILSEIPITVVRGNASEVMSLFAGGEKIKIRGVDSMESVEAVRHGAT
                     VMAAELRKVVAVTGEIDFVTNGHSSIEIHNGHPMFRRVTGTGCGASTAISCFCAVEPD
                     PLVATAAALGYYGLAGEEAAKVSNGPGSFQVALYDALYDLSQKTMMEKLRIRPVR"
     gene            complement(23453..24649)
                     /gene="nifS"
                     /locus_tag="PK125_02300"
     CDS             complement(23453..24649)
                     /gene="nifS"
                     /locus_tag="PK125_02300"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR03402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase NifS"
                     /protein_id="HPB36973.1"
                     /translation="MKTIYLDNNATTQVAPEVLETMLPYFSELYGNPSSMHSFGGQVS
                     KTIQHARENVASLIGADPEEIIFTSCGTESDNTAIMSALSVAPDKSHIITSRVEHPAV
                     KVLCEHLTQRGYRVTELPVDRDGRLDMELFENSLSRDTAVVSLMWANNETGVIFPVER
                     AAQLAHERGILFHTDAVQAVGKIPINMRANNIDMLSFSGHKLHAPKGIGVLYIRKGTR
                     FLPFMLGGHQEKGRRGGTENTASIAGLGKACQLAARNVETENTRVKYLRDKLEDELLK
                     SIPNSKVNGDRTNRLPNTTNISFEFIEGEGILLLMDQFNICASSGSACTSGSLQPSHV
                     LRAMGVPFTMAHGSVRFSLSVFNTEDEIDFVINKLPPIIARLREMSPYWKTESPVCGI
                     RDTATQ"
     gene            complement(24646..25485)
                     /gene="nifU"
                     /locus_tag="PK125_02305"
     CDS             complement(24646..25485)
                     /gene="nifU"
                     /locus_tag="PK125_02305"
                     /inference="COORDINATES: protein motif:HMM:TIGR02000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0009399 - nitrogen fixation [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein NifU"
                     /protein_id="HPB36974.1"
                     /translation="MWEYTDKVKEHFINPRNVGEIENPDGVAEVGSIACGDALKFTFK
                     LDENKRIKEAKFKTFGCASAIASASALTELVKGMTLEEAIKVTNKDIADYLGGLPEEK
                     MHCSVLGQQALEQAIANCQGAPAKEPGEQIVCECFGVTDREIEKAVRENHLTTVEEVT
                     NYTKAGGGCTNCHEKIQAIIDRVNITSEPEGLKEPKQKKLTNIQKIRMIEETIEREIR
                     PSLKQDGGDVDLIDVIGNRVIVATRGACATCKASQFTLKGLIEQKLREFVSPGLIVEE
                     EPK"
     gene            complement(25613..26641)
                     /locus_tag="PK125_02310"
     CDS             complement(25613..26641)
                     /locus_tag="PK125_02310"
                     /inference="COORDINATES: protein motif:HMM:NF019845.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase family
                     protein"
                     /protein_id="HPB36975.1"
                     /translation="MKAMILNKITNLRENNAPLELVDFPKPFPEDKDLLVKVSACGVC
                     HTELDEIEGRTPPPFFPIILGHQVIGRVEVTGKDVKKFKIGDRVGIAWIHSACGTCMH
                     CREGNDNLCDKFEASGRDAHGGYAEYTTISEDYAFPIPGVFSDLEAAPLLCAGTIGYR
                     SLRLTGMKDGDILGLVGFGASAHLVIQMARHNYPNSLIFVFARNESERAFARELGAGW
                     AGDIGETPPLKLNCAIDTTPVWKPIVKTLANIAPGGRLVINAIRKEDIDKEQLLNLDY
                     SIHLWHEKEIKSVANVARQDVRDFLELAAAIPIRPEVQVFRLEDANRALLELTTRKIR
                     GAKVLNLQ"
     gene            complement(26799..27611)
                     /locus_tag="PK125_02315"
     CDS             complement(26799..27611)
                     /locus_tag="PK125_02315"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HPB36976.1"
                     /translation="MPDYREIDNSPALNYIFYPRSTHNKPPVNAFDKTIAVDGNVVII
                     CRFYKGNDDWPWIVFFHGNGEVVSDYDNIAPFYNKRELNLVVVDYRGYGASTGNPALN
                     YLAHDAYILFRDIKEDVLQLSHENNIWVMGRSLGSISALELGHHYSDQIRGLIIESGF
                     PSITQLILHLGLPAGSIDLSTVYDECISLVRKITVPTLIIHGEYDILVPLREAEDLYA
                     NIGATDKELIIIPGADHNDIMFSGFDEYFGAIQRFIKRTGTKPCLDHSAKSL"
     gene            complement(27719..28492)
                     /locus_tag="PK125_02320"
     CDS             complement(27719..28492)
                     /locus_tag="PK125_02320"
                     /inference="COORDINATES: protein motif:HMM:NF013757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HPB36977.1"
                     /translation="MNDDQKKYSVRVLEKSIKIFDIFSYGHDSFTLSEIKKKTGLPKT
                     TVFRILKTFERAGFFKYDPDQETYSLGLRFLELGGLVYSSLSVRKAAAPYMDALSNSL
                     KATILLGVMKDDQLFYIDKREMDSIIRVTSHIGLKRPPYFGMLGMILLAHMDSKEINR
                     LLNLYPPTKITPKTITNMDQILENLEKAKKQGYYVEQDEIVEGLLGVGVPIRDFSGNV
                     VAALGATQPVFQIKDGTIENTIRQLLEASRSISKDLGYT"
     gene            complement(28717..28869)
                     /locus_tag="PK125_02325"
     CDS             complement(28717..28869)
                     /locus_tag="PK125_02325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB36978.1"
                     /translation="MDEIRNEIMNIEKSAEKLKTLAKDNNAIRKNAEIILTFLYILKF
                     ITPATD"
     gene            complement(28869..30176)
                     /locus_tag="PK125_02330"
     CDS             complement(28869..30176)
                     /locus_tag="PK125_02330"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HPB36979.1"
                     /translation="MEFWNPYTETLPREKLEKIEFSYFQNLLSYAKANSRLYKDKLKG
                     INIDDIKVFEDIKKIPFTYKEELRKWQEDVDPFPYGGLLGIDIANVSSFRQTSGTTGK
                     PVYVPESYESWQWRVESWCHILYMAGFRPRHRVFLPFGYNVYVAFWEAHYAAEKLGCE
                     VVPGGALDTKGRVNKIIELKANAMCGTPTYLLNIAQEAASMGIDPKSLGIERALGAGE
                     PLPEATRKRIEETYNCKMYDHIGSTETCGFAGMCEAQQGLHIVEPFFLVELLDKDTLS
                     KEVSEGELGVIVITPLGRRSFPVIRFNTNDVAIKGPSTCECGRTSLKLMEIVGRVDDI
                     SKIRGVLFSPKTVEQLIRSEFPEIVEYELVVTREDIMDKITLRIEADPKLNKDEAGEV
                     AKRLGERHKIKTNLSCKVVVEPQGTLPRYDLKAKRFKDMRGKH"
     gene            complement(30296..31936)
                     /locus_tag="PK125_02335"
     CDS             complement(30296..31936)
                     /locus_tag="PK125_02335"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HPB36980.1"
                     /translation="MAIEGFTEYAKEDREKYNRLRWWLGLTWGDLFDKATDIYPDKIG
                     LVDGTGRWTYGQLREKVDRLAISLMKRGIKPKDWVLLQFPNWHEYIISFFAMQKIGAL
                     TLLLIPRHNQSEINHLCALTKPVAWIVPKQYGKIDYEPIIDDVLKENPQIKTIIQVRT
                     GKKGRYPSLDELIEEAELTPENLKALEERRPDPDEVSHIMPTGGTTGLPKASVRTHNC
                     YICNVEYHSRVWEITSNDTLMVVTPVGHSMAMHWGIGAAFLNFSKLVLLDSTQPEDIC
                     EWIQKEKVTAIPSVPALIARIINMEDIERYDLSSLKKISVGGAPSTPELLRGAYEKLK
                     CIVINGFGSSEGTNTATRPGDSMDIICNSVGRKACPHDIIKIIDDSGIEVPPGVEGEL
                     VSKGPGIFTGYFKSQEENKETFTVDGFFKTGDKARKDEFGNITITGRIKDIINRGGEK
                     ISALEIEHRLSAHPQIQEAAVVGMPDKILGERICAYVVLRSGEKPTFDSIIAFLKANG
                     ASVQQLPERIEFIESLPATKVGKVDKKALREDIKKRLE"
     gene            32214..33023
                     /locus_tag="PK125_02340"
     CDS             32214..33023
                     /locus_tag="PK125_02340"
                     /inference="COORDINATES: protein motif:HMM:NF013757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HPB36981.1"
                     /translation="MKSNGEDTGKSFYNRSLERALQILNALSDERPTLSLAQLSETLN
                     LSRATVLRLCSTLLDFGFLRQDPMSKHYSLGLRILDLAKSVSDSFSLTKIASSHLKIL
                     QVKLGKTVFLSVLDQDEVLYIDKLDDPKNPISFTSKIGTRRPPYYGMMGSILMAYLPH
                     GEIKRILHKQPLKAFTQKSITKKQEFLKWLGKVRLQGYVIDTETALEGISGVAAPVFD
                     YTGKVVAAVGVGFISSSVKEEEMAKIVEETIRTTSAISKELGYKEKKNEST"
BASE COUNT         9021 a         7074 c         7519 g         9364 t
ORIGIN      
        1 aatcccctgc tcttcaagct cgtcaactgg aacagtatag tagtctttct cgtaaatcga
       61 tgaaaggatg tcgaccacac cctttttgat cccctctggt gttataccgt gttctttgtt
      121 gtactgtgcc tgtacttttc gcctcctctc cgtctcttcg atagcccgtc tcatggaatc
      181 ggtcactatg tcgccaaaca tcagaacacg gccatttatg tttctcgcgg tgcgcccgca
      241 ggtttggatg agggaggttt gtgaccgcaa aaaaccttct ttatccgcat cgaggatcgc
      301 caccagggaa acctcgggca gatcgagtcc ctccctgagg agattaactc caaccagaac
      361 atcaaatttc ccttttctca aatccctgac aatggcgacc cgttccaggg tgtcgatgtc
      421 ggaatggagg tatttggcct tgatgccacc atcaaggagg aagtcagtta aatcctctgc
      481 aaatcttttg gtgagagtgg tgatgagtat ccgttccccg ccttcaatga cagccctaat
      541 ttcagggaga agggcatcta tctggttttt cgcaggtttc acttcgatct ttggatccat
      601 gaggcctgtt ggtcttatta tctgctcaac aacatgtgct tttgcctttt tcaactcgta
      661 tgacgcaggt gtggctgata tgtagagaac ctgtccctct cgctcttcga attcttcaaa
      721 agtgagtggc ctgttgtcga gtgctgacgg caacctgaac ccatactcta caagtgtcgt
      781 ttttcgtgac cggtctccac gaaacatgcc aataagctga ggtatggtaa cgtggctttc
      841 gtcaatcata atcactgcgt tctctggcag gtagtctatg agggtggggg gaggttcacc
      901 cggcagtcta ccggtgaggt ggcgtgagta attctcaatt cctgaacagt agcctatctc
      961 ccggatcatt tcaagatcat acttggtcct catttcaaga cgctgggctt cgaggagctt
     1021 gttttgggaa cggaggagga aaagccgctc cccaagttcc tcctcgatcg tcaggatagc
     1081 tctttcgatg gtatggcgtg gggttacata atgggttgcg ggaaaaatgg tacaccttct
     1141 cagcctgtta atagttttcc ctgtaagagg atctattgtg tggatggatt cgaccctatc
     1201 gtcatcaatg ataatccggt atgcattctc ctcctcatag gctggaaaaa tatcaatatt
     1261 ctctcctcgg attctgaact tgccccggta aaaatctgtg tcatttctct catactgaca
     1321 gtcgataagc ttgtcaagga gcatcttccg tgtcatattc tggccttgtt caagaacgat
     1381 aagcatcccg tagtatgcct cgggagagcc caggccatag atgcatgaca cgcttgccac
     1441 aataatgaca tcgtcatgtt cgaaaagggc atttgttgcc aaaagcctta atttgtctat
     1501 ctcctcgttg atagatgaat ctttttcaat atacgtgtct gtctgtggct tgtaagcctc
     1561 gggttgatag tagtcatagt aactcacgaa aaagtggacc tcattttccg gaaaaagagt
     1621 cttaaactct gagtagagct gggctgccag ggtcttgttg tgggatatga ccagagtagg
     1681 gcgctggaca cgctcgatga cattggccat tgtaaaggtt tttccggaac ctgttacacc
     1741 aaggaggacc tggtggggaa caccatcaac caggtttttt gctatttttt caattgcctg
     1801 cggctggtct ccttttggag cgtaatcgct gatcagtttg aaattgggca tagtcttata
     1861 ttttaatgcc tgtgacttgc cgtgtcaaag acagcaagta ataataacat tagagatgat
     1921 attgaacagg atagaatttt attgacaaaa atgttgggtt gatagataac tatgacagat
     1981 tgtggcaaca ctgctgattt acttggttgt tttgagtact cagcaagaaa tattttatga
     2041 aggggtaata tacgttcaaa cttttgccat cagacctgct gttatcccat tttccaccca
     2101 ccagatatcc tgtcttcaac gcatatttct tcaatgattc attttctttc cggcaggcta
     2161 ttttatcatg atctatacta tcaaagactc atcgcttcgg gtatacagct ttcttgccaa
     2221 tggtcacata attatctctc gaagtattag cttcaggttt agctagggca gcattctcaa
     2281 aaaaagaaag ggtttaatat gacttcatcc agggagtgac atggaatttg atgtttattt
     2341 tccagaatag tgaggtccaa caatatgcgg cataaaagga ggtgaaaaaa gtacggaagt
     2401 ttcaggtagt tacaaaatca ttcaaaagga ggattcaaat gaagagttta agtgtaaaaa
     2461 ttattttggc ctttgcttta ataattgggt ttgcctatgg taatgcgtat gcacaggggg
     2521 tagacttttc tatttgggat ggaagctatt ggagtaccaa gtttacagca aaaggagttt
     2581 tattttccga ccctgccacg ccaccgaata aaaaaatcaa tgggactgaa aagtcatggg
     2641 ctataatgat atcgagtgct gatggatctg aaatgacttt acagttttat caaaaaaacg
     2701 ataggggtga atgcgttaag ggagatgaaa taccgatcac cttgatgtac ggagggccgc
     2761 tggggttcgt ggctactatt acaattgacg aaccagaacc ggactttaat tctgctcggg
     2821 tactattata ttttacagga aaactgaaag gagaggcgct gacaagcggc aagattgcaa
     2881 cactgggcgg gtacgccatt gaacaggact gggatgatgt aaccgaccgt gccgccttcg
     2941 gtgttaccct ctcaggcact cttttgaaag aaccgaaggg ctgtttattg gacaaataac
     3001 attcaggaat gaaaaaggcc tttgaagatc gaggggcatt tatactgccc ctcgatcttt
     3061 tatattttta gttcctgaaa acaatgccac taaattccgg gtgaaggcac tacttctttg
     3121 ataaaatctg ttatctcttt ttcattgccc tggtagaaat gatcggtggg aactatttta
     3181 agtgatttct ggagagaagg tatcgccttg tatgtctcta tgagccggtc aataggtccg
     3241 atgtcatctg attttccacc taccaagaat attgtccctt tgtatctttt taatgattcg
     3301 ctttcataga aagcaaaggg atatgaaacc aaaaaaaggc catccagtct ttccagtttt
     3361 ggtgctgcct ttgagcatat ccatgcgcca aaggaatagc ctgcaaacat gatcagcccg
     3421 tctttgtcga tctgctccat aaggaattct gcagcatcca ggaggtcttc ttcctcaccc
     3481 tgtccgttgc cgtagaaacc tgtgctttca cctacacccc ggaaattgaa tcttagcgtt
     3541 gtaaatcctt tgtaggcaaa gcctttttca atcgcaccta caacgttgtt gtgcatgtcg
     3601 ccaccgtaga gggggtgggg atggcagata acaacgccgc ctctcctgct gttttcattg
     3661 atgattccct cgagcttgca cgctccctct atgatgaccc tttttattga catgacatgc
     3721 ttattctggt agttttctaa caaaaattca acaggaaaat tcgattgatg ggatttttta
     3781 caagaaaaga aaaacgttta aagaagaagc cacacaaagt catcgacatc cagaaggaga
     3841 tgaagcgtgc agaaacggac gagtctctct ggattaaatg cagttcttgt gaagggcttt
     3901 tatacaagag agaggttgaa agaaatcagc atgtttgccc caagtgcttt tatcattttc
     3961 ccctcaccgt agaaaacaga atcgccacaa tttttgacaa aggtagtttt acggagcttt
     4021 tttcgaatat agagcctgtc gactttttaa agttcaaaga tacaaaacca tataagacca
     4081 ggctaattga gacaaaagag tcaacaaaga agactgacgc tatgatatgc ggcaaaggaa
     4141 aaatcaatgg atttgaagta tatacagcta tctttgactt ttcttttatg gggggaagtc
     4201 ttggatcagt tgtgggtgag aaggttacca gaatcctgga ggaaggactc aatcagaggc
     4261 tgcctgtaat catcttttgc tcgtcgggtg gcgccagaat gcaagaaggc atcatgtccc
     4321 taatgcagat gtcaaaggtt tcaggggcga tttacaggct caaatccagc agaattcctt
     4381 acataactgt tttaactgat cctaccctcg gtggtgtaac cgccagtatg ggaatgttgg
     4441 gggacatcat tatttctgaa cccaaggcca tgattggttt tgcagggccg agggtgatca
     4501 aggatactat caaagaagaa ctgcctcagg gatttcagcg agcggagttt ctttttgaac
     4561 acggcatgat cgatatgatc atcccgcgga aggaactcaa gcagagattg cacgccatac
     4621 tgtccatgat aacatgacag aatacacaaa tgcccttgca tacctctata accttgagaa
     4681 gtttggtatg gtatttgggc ttgacaatat cagatggatc ctcaatctta ttggaaatcc
     4741 ccatgaattt ataaaagcag tccatattgg cggtacgaac gggaagggct ctgttgctgc
     4801 catgctctcc catgtgttta aagccgccgg atacagggtt ggcaaataca cttcccctca
     4861 tcttgtttct ttcgccgaga gaattacaat tgacgaaaaa aaggttacag aggcagaggt
     4921 tgcggatttg gtcgtgtcca tacggcaggc tgttgaagct gtagatccga ggagaacttt
     4981 tacctttttt gacttcacta ctgcccttgc ctttgaacat ttccgtaaaa atcacgttga
     5041 ccttgcactg attgaggtgg gcctcggcgg gagacttgac tctacgaatg tgataaatcc
     5101 gttggtctca gttattacaa acattgccta tgatcacatg gattaccttg gagaagatat
     5161 tcttcgcatt gcacgggaaa aagcagggat cataaagaga accgtgccgg tggtcacagg
     5221 tgcacaggat ttgccgggac agatcatcga agaaacagga agattcaatg atagccctgt
     5281 ctatgtcatg gacagagact ttttttacga aaagaaagga actcagtcca tgacctattc
     5341 tggtctttca ataaaacttg atgatgtgtt cgtcaatctg aaaggagacc accagttagg
     5401 caatgcagct attgcactgt gtacatcaga gcttctttct atgcttggtt tcgggatgga
     5461 ccggaggagc atttacgagg ggctttcgca ggtaacctgg cctggtaggc ttgagattat
     5521 aaaagagagg cctgagatta tcctcgatgg tgcccataac gttcatggaa taaagactct
     5581 ggcaagtttc ttaaaaaacc aattcccaga cagaagaaga ttgttgattt ttggcgttat
     5641 gaaggataaa gaatatgaga agatgctcga tgagctcctt ccggtggtcg atgaagtaat
     5701 ttttacatct tcttcaaacg atagggcatt acagcctgaa tcaatgaaaa aggttgctcc
     5761 ccgtgcagtt attgcagacg atgctgcagg ggcgctcagg aaggcaaagg caatgtcagg
     5821 cggaaatgat gtaatcatcg tgacaggctc tctatattta attggtgagt gtagagctat
     5881 tattaatgat attttctaaa aaagctttaa tatttctcat aattgtatgg ttgttcatgc
     5941 cggtctttgc gtcaggtaag aactcagcaa tcagtttgcc gaaatttact ataaccactg
     6001 agcctgttga aatatcagca aattcattgg aatatgacaa aataaacaat gtttacaaag
     6061 ctaaaggcgc tgttgatttg caggaaggaa ctaggcggtt aatggcagac gaagttgttt
     6121 acaatatgga ttccggtgat attgcagctt cgggtaacat agtgttccag gatggggaag
     6181 atattattaa atgcgagagg ttgagtctca accttgaaac gaagactggg acaattgaaa
     6241 agggtacaat ctatataaaa cagaataact tcactgtaac aggcgaacag atcgagaagg
     6301 tgggggacca gcaatacaaa ataaagaaag gacaatttac cacatgcgat atgcctcaac
     6361 ctgattggaa gttctctgcc cgcgatgttg atatcacagt tgaggggtat gccaagacaa
     6421 aaggaacgcg atttcatatc cttgaccagc ctgtgcttta tttgccctat ggtctctttc
     6481 ctgtgctgac aaaaagacaa tctggatttc ttatgcctga ccttactgtc tcaagcaggg
     6541 acggaataaa aataaaaaac agctattttt gggctattga cagggataaa gatgcaacta
     6601 tcggtttgga atacattgaa aagagaggat ttaagccgga attggagtac agatacgact
     6661 tgaaggaggg cctgaagggt gcctggaact ttgcaatcat tgatgatacg aaatacgacc
     6721 actggcgtta tgaaattaaa ggcaaacacg aacagaccct gtttaaagac ttacgcctca
     6781 aaacaaatat tgactatgtt tccgattacg cctatctgat ggattacagg caaggccttg
     6841 atgaacgaag tgaaaaccat ttgaagtcaa cggcctatct tgaaaagccc ttttcacatt
     6901 cattacttac tgtcgaggga gcctatttca gggatctttc aagaaaggat aatgataata
     6961 cttttaaatt ctatcctcac gcaacctttt tcacagatta cctgcctctc atcaggcggg
     7021 ttttatatgc tgatatctca gcagaggcgt ccaactttta cagagaggaa ggaaacacat
     7081 attcacgcat cagcatagag cccagactca ggcttccatt ctcgtggaac ggggtgaatt
     7141 tccttctatc aggaacgctt tttgagaaat tctactatat tgatgagtca aagcccaaag
     7201 atacctccac gaaaaatctt ggaactgcga ggtttgatgg ttctacgaat gttcagttca
     7261 tgagaaatta taatgttgat ctgtggaata ttgggacagt gcagagcgtg ataaggccac
     7321 aggtccagta taccttcata tccaacaact cttacagaga gataccatta atcgatccct
     7381 ttgacaggat tggaaaaaca aacaccataa cctattcgct gagtcactat cttaacacat
     7441 ttactccaca ggtcggaggg agagagattt ctctcctcga ggtatttcag acgtacggaa
     7501 tgtctggaaa ccttccccta tcagatgcgt acaggggcta tgggaaccgt ttctcagaca
     7561 ttacgggaag gctgaccctt tttatgaaaa agaatatttc atttacaaat gaagctgcgc
     7621 ttaatgtcca tggtgaaggg attactgcaa caagaaatgt actacgctat atttaccctg
     7681 aggtttatta catgaatata tcccataact atacccagag tctggcaaat cagatggacc
     7741 tcgacattgg gggaaaatac aaatttttaa caggaaggta tcgcattatt tattcattca
     7801 agaataaaga gtggatagac acacagtatc agcttattta ccaaccacaa tgctgggcag
     7861 tgattctcac acttaagcaa acaaagaggc ctaacgatac aagtataaac atcggtttcg
     7921 atcttaccgg tcttacgggg aaaatagatg atattaataa aacattcgga atatatcaga
     7981 gataagagct atctaaccaa ggaggttatg ttatgaaggg cgttattcta gccggggggc
     8041 ttggaacaag actttcccca cttaccaaaa ttacaaataa gcatttgctc cccatataca
     8101 acaaacccat gatctattat ccaatagagg ctctcataaa ggcaggaatt acagatatca
     8161 tggttgttac cggtgggaac catgccggtg attttttgcg actcatggga aacggaaagg
     8221 agtttggcct gaagcatatc aattacacgt accaggaagg tgagggcggc atagcagatg
     8281 ctctgtctct tgctgaatat tttgctgaag gcgagcccat atgtgtggtt ctgggtgata
     8341 atatcattga aaaaaatatt attaaagctg ttaaggattt tggaggacag aaaaaagggg
     8401 caaagattct cttgaaagag gtacctgatc cagaacggtt tggcgtagca gagattgtga
     8461 atggacggct ggccaatata gtagaaaaac ccagacagcc aaaaagcaac cttgctgtta
     8521 tcggcatata tctgtatgat agcagggtgt ttgatattgt taaaaccctc aagccatctg
     8581 acagaggaga gcttgagatt acagatgtga ataatgcgta tgtgagggaa ggtaccatga
     8641 catgggagat gttgcagggt tggtggacgg atgcaggaac attcgaatca ctactcaggg
     8701 caaatgttct cgtggcacag acaggtgcga ataatgggta gcgtttagct gtcagcaacc
     8761 agctttcagt taaaccataa accatatggt aatgcttttg acctgagagg taattattgc
     8821 agcagttgta tgtaaaattt tgttttaaag tttatagctg agagcggaga gcgtagtcaa
     8881 actctatgcc ctttgcccca tgcattaggc atttatgggg aggtgcacat gataaaagga
     8941 gttgcaacca agcaattgaa gttcattcct gatgagcgag gaaggctcat ggagatattg
     9001 cgaaatgacg atgaaatgtt catcaagttc gggcaggtat acctcacgac cacctaccct
     9061 ggtgtggtta aggcatggca ctaccacaag gagcaggatg attatatcac ctgtataaaa
     9121 gggatgctca aactggttct ttatgatagc agggaaaggt ctgcaacaaa aggagaatta
     9181 aatgaattct tcattgggga atacaacccc atggtagtaa aagtgccggc catgatttac
     9241 catggttgga aatgcataag tgatgaagag gctattgtga ttaacgttcc aacagaagtg
     9301 tatgacaggc aaaagcctga tgagtatcgc ctggaccccc acaaaaatga tataccatat
     9361 aactgggaga gaaaggatgg ataaaaccag gatacttgta acaggtggtt gcggttttat
     9421 cggcagtaac tttattaggt acatgctttc aaaatacccg tatatcatta tcaattttga
     9481 taaacttacc tatgccggga accttgaaaa tctcaaggat gtggaaagtg attcacggta
     9541 cacttttgtc agaggagata ttgccagcaa ggcagatctg gcatctgtat ttgagcaggg
     9601 aatcgatgtg ataataaact tcgcggcaga atcacacgtg gacaggagca ttctcgaccc
     9661 agacaccttt atccgtacaa acatcaacgg cactttcaac ctgcttgaag aagcaaggaa
     9721 gagagaggtt aaaagattca ttcaaatatc gaccgatgaa gtgtatggtt ctcttggaaa
     9781 agaagggaag ttcacagagt ctacacctct ctccccgaac agcccttact ctgcatccaa
     9841 aaccgctgct gacatgcttg ccatggcata ccacaagact tatggaacac cagtgctcat
     9901 aacccgatgt tcaaacaatt atggtcccta tcagtttcct gagaaactga taccccttat
     9961 tattacaaac gcacttgaag ataaggagct tcccgtatat ggtgatggca tgaatgtaag
    10021 ggactggatc catgtgattg accacgcagt tgctatcgat gctgttctcc ataagggcgt
    10081 actgggaaat gtatacaaca ttggcgggga gaatgaacgg accaatattg agattgtgaa
    10141 gcttattctg agtattcttg gtaaaccaga gagccttatc aaatatgtca cagataggcc
    10201 gggtcatgac agaagatatg ccattgattc atcaaggata aagagagaac tcacatattc
    10261 tgtgaccacg gattttcgga aaggtatgga agatactgtg aaatggtacc ttgataatag
    10321 aggctggtgg gaacgtatta agagcggtgc atatctcgaa tactatgatc tcatgtataa
    10381 aaacagatag aaggaaagtc ctttgaaaat actcaccact ggaagtcatg gactgctggg
    10441 cacgaacatc ctgcctgttt tagaacgctc gtttactgtc attccctatg atattgagga
    10501 atgggatatc actgacaggg acgctggtga acgaacaatc agggatattc gccctgatgt
    10561 tattttgaat cttgctgcct atacgaatgt ggatgagtgc gaggactgcc tgagccttgc
    10621 ggaccgagta aatgctgagg cgccgggaat tatagcaaag ctttgcagtg accacaatat
    10681 caagtttgtt catatcagta cggattatgt atttgatggt gaaaaggaaa cgccatatag
    10741 agaagatgac gagacaaacc ctacatctgt gtatggcctg agcaagctct caggggaaaa
    10801 aaaggtcctt gccaactgtc ctgatgcaac tatcatacgt actcagtggc tctacggaca
    10861 tggtggaata aacttcataa caaagataac caaaatagca agagagcaag gtgtggctga
    10921 agttgttaat gatcaacgtg gttgtcctac ttatgcaaaa gatattgctg atcctatcct
    10981 gcttctgata gaaaaaaaca ggtcaggtat ctaccatgtg gcaaatgcag gttcgtgcac
    11041 atggtttgat tttgcccggg agatattctc atgtcttcac attgatgtgg ttctaaagcc
    11101 aatcacctca gaacagttga atagaaaagc caacaggccc cggaattcag tctttgactg
    11161 ttccaggctt ttcagagaca ccgggcatcg catgaggacc tggcaggatg cgcttcatga
    11221 ttacctcagt tgaataatca tgaagatgat cttttttgcc gatacccatc ttaccaaaaa
    11281 gaataagaag aaactgcgga tggttgagat ttttatcagg aacgtgtgca tcgatgcgga
    11341 tatggtcttt gtcctgggag atctttttga gttttaccat gggtatgagg gatatatcta
    11401 tccatggtat cgacctattg tcgatgcctt taaaacaatt acagaacagg gaaaatcagt
    11461 ttactttcta gaaggtaacc atgagtttga tatgggccct ttctttgaga gctacactgg
    11521 tattcaatgc gttaaaaacc tcaccataga tattgaaggc aaacgtacct ttatctctca
    11581 cggtgatgag ttttatctga atcttgtgac aagggcattg aaggcacctt tgactattgg
    11641 cgccatgaac ctttttggcc ccaggctttc atggaaaata gccatggctg taagcagaat
    11701 catgtcaaaa aaaaagaaag ggcagaaccc gaaagttaaa aatgtgttcc gtgaatatgc
    11761 cagggaaaag cttaaagaag gttacgacgc agtcatcctt gctcacactc acattgcaga
    11821 ttatatggag tttggtgctg ggatggcaaa aaaggtatac ctgaacacag gagacttcat
    11881 cagaaaatcc acctatattg agtataccag tgaagagggt tttcagttaa aggaatacaa
    11941 accgttaatt agaggaatat aaccttgaag tcgtcacgtt gctttgcaac acgctttaag
    12001 agagaagcaa tcttggaata gtcctttttc aatgccttct ttgaccctga cgatgactcc
    12061 catacaatct cgaacggtcc tttaatgtcc gtcgtaagaa tatctgcaag agcatcaagg
    12121 ttacggccaa aatgttcggg gagggaaagt gcttgaacaa tctcatcata aaaatcattg
    12181 agcgactgga ttgccctacc gtttaaaaca tattttgtca ctgacattcc tgtacctcac
    12241 tgaaagtctg gtagtgatca acggtaatca ttcgcaggcc atccggtgaa aagacaatac
    12301 gcttcggtcc cctgcgtccg ccatgatagt cgaggtctgc ctcccgccac ttacgtttgc
    12361 cattgggaag tttcttttca taattgccga acctgtctcc gccgatgctc tttcccctca
    12421 gtctatcaac cgaccataat gatgtgccgg gtttccatcc agcctgagat gcttctttct
    12481 ttgttatgaa ttttttcggc aactcccgat tatttgttgt gttaagggag cggaggatgt
    12541 caataagctc atttgtatca atcttttgat tcagttttgc attcaagtca tctacaacag
    12601 tttcacagtt caccgcatga cttgtggtgg agaaaagaaa acctgcaaaa acagccaatg
    12661 caacagtcaa acaaacatgc gttatccttc caatcattat cacatcattc ccgctacaac
    12721 aatttcctgt tgtttgtatt caatcttttt cacaatacac agttgcaaga caatgaactt
    12781 ggcaagaagc cagggagata tgctctcccc ggcttacttc agttcggtta cttagaaggt
    12841 gccggagcta ccggtttctc tgccttcttc tttgcacggg ccttcttaga accctttagt
    12901 cccacatcct tcgctgcctg ctcgctgtgg cctcgtttct tccagatacc tgaggattgg
    12961 gttctcttca ggtacggtgt agcacagagc acggatttat cagcttcaaa ttcatgagaa
    13021 accattgccg ttctatccag ttttgtgnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    13081 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    13141 nnnnnnngaa aaaatatctg atatgatgct ggaaaaggaa aggcaggatg tatcggaaag
    13201 gatgggatag aatggaaatc attaactgaa tacaagcaaa gaaacacata ccctatggcc
    13261 agcaggaaag ggcacaagga gtgtcatgat tatgagtatg aactgtgtgg gatatttaac
    13321 gtagcttttg caaataaact gttggttggt ggggagtgat gacgaatcga aaaacaaata
    13381 gagaagtaaa attccctgca gccgcataga atttgtgtat accccaaagc atggcagtcg
    13441 gctcaacatg gccaagattg aactgaatgt cttgctcact caatgcccaa aaggcccatg
    13501 aatttccttg cacaggtaaa agaaaaccat ttcatcatag caagcctgct gtgttagcca
    13561 ctaaaacgat cgattgacgt tttctctatc aggatacccg tataaaactg cagagtcttt
    13621 atccaacatt agatgattga cttgagagtc ttggtcactg ttgcaggggc tttcgtcgcc
    13681 tcaaccatca ccaggggtgt ttgagcctca ccttcacgcc catggtactc gctctatagc
    13741 ttcccacgaa agaggcaagc cacaaaagag aaccaccctt tcaaggatgg gtgaaccgac
    13801 ttatcattca cccacagcaa gtatattttg tgctgcaggc ttacctgtgg gaatctgttc
    13861 aaccgcaaca aacatcctgt tttatagact attaagatta taaaatggct accagcagaa
    13921 gataaacgat gtacaccgta tcgaaccgat ggattcatat gtcgattctg aatatggcaa
    13981 gtgtgggtac gtctttccct cacagataaa attgaatatc tgtgagggag caaattatgg
    14041 taatattgtt tgcacattct gttcgtacag ccataaaggg aggtactatg ggcaaatcag
    14101 ttaaagggga aaatgcaaaa ggagcatgtc ggagggatat ctcggtcatg tacaatttcc
    14161 gggatgagat accaggtatc gttgatacta ttgttgccct tcatgatcag gatgacaggt
    14221 tttgtcatat cagccctgaa cctcctcctt caagaatgct ggtcaacgaa atcatccgaa
    14281 ggggtatgcg catactgtat cccggttatt tctctgaaac atgcatggat gaggtaaaca
    14341 tgcggtatta tcttggtcag gagattattg agttttacca actcctctca gaacagatta
    14401 tcgctgccat caggcacgat tgtgtccgtt atgatcttgt ttgtaccaac tgcgatgaaa
    14461 ggggccagcg tatagcattg caattcctca aggatatttc caatctgcag gctgtgcttg
    14521 caaaggatat cattgcagcc catgagggtg atcctgcgtc aaagtattac gatgagataa
    14581 tcttcagcta tcctggcctt tttgcaatta ccgtctatcg tatggcccac aatttgctcg
    14641 aacagggagt acctcttatc cccaggataa tgactgaata tgcccataat attacgggaa
    14701 tcgatatcca tcccggtgta catattggag agagtttctt catagaccac ggcacgggcg
    14761 ttgtgattgg cgaaaccaca accataggag accgcgtgag aatctaccag ggtgttactc
    14821 tgggcgccct gtccctgagt aaagaagaag tggagcgatt aaggaacaag aaacgccatc
    14881 ccaccattga ggatgatgtg atcatttatt caaatgcaac gatactggga ggaaatacag
    14941 ttataggcgc ccgttcggtt attggcggta acgtatggat cacagagtca gtgccgcctg
    15001 acacaacagt gtttctgaaa aagcctgaat tagtcattac aggaaaggcg aagtgatgtc
    15061 ctggagcaac tgtgatgcct ctttcgctgc gactgtttaa agaagattca tcgcaggata
    15121 atttgacctg tgaaatatag ggtattctga actaggagga aaaatggaaa taggagaatt
    15181 acaaaaaggt aaagataaaa ttatcatcgc actcaaggaa ttcaaaggaa aagagtacat
    15241 cgatattaga acccactttg aaaatagtga cggggactgg atacctacca agaagggggt
    15301 gaccttttct cccgagaacc tcgacgaaat gattgaactt cttatggctg caaagaagaa
    15361 aatgtcagaa agataaaaac agtacctccg agacgatgaa atacataatt gatttcacac
    15421 catgtttcag atgcagatcg ggaaaaacag cggcaaggct actactgtct ggcttcgtca
    15481 accatgatca gttatatttc ccgggtttgc aaaccaccaa tttcctgtat tcttcattct
    15541 ttcagtacct tacccctgcc ttgaggtatg gatatcaacc catctcgcaa aggcctgcat
    15601 gaattttgtg acaaactctt caattgaatc atttgcaagg tttccgcgct catcgaagag
    15661 attggctgca ttgccgatgt aggcctcagg ctgttgcatt gtggggatgt tgagaaaaac
    15721 cagcgactgt ctgagatggt ggtttgcccc gaacccgcct attgcaccgg gtgaaacaga
    15781 aattacagca ccaggcttgc catcccatac actttttcca taggggcggg agcccacatc
    15841 gatggcgttc ttgatcaccg caggggtaga ccggttgtat tcaggcgtca caaacaagac
    15901 accgtcaaac ggttttaccc gttttctgaa atctgtccat gaagcaggtg gttttccctc
    15961 atcatcaagg tcctggtcgt acagtggaag ccctctaatg tctatgattt ccagctccag
    16021 tgtttcaggt gcatgtgaag caagggcttt tgccattttc cggttataag attccttgcg
    16081 gagactccct acaaaaactg caattttttt tgacatttga attccctccc tccttcatat
    16141 tgtttagata agtatagggg atgctgttaa acattgcgag atgtttatgt tatcttaatc
    16201 cctctatatc tggaaagata tctgagaaac aatagatgtt ttttcttgtc ccatcaataa
    16261 gcagcaccgg gtgacaggcg ccatcctcga gccgttttat tgttatggta atttcccgtg
    16321 tttccgtgtt ctgttcagcc aggataatgg cgcgaatgac aagtggtgat aggccttcaa
    16381 taggctcatc atcaatgatc agtccgcaac ctcccccaag cgcgactttt tgcacgtgca
    16441 ccatgttgtc ttctctgttc gtatacagag tgccatccat tccgtcaaga ctgaagctgc
    16501 ggccgaaaat gtttgatctg caggttatcc tgaactccct gttgttttgc atgacattgt
    16561 aggtggaaat catatcagaa gcactaccat ccacctggtg acagtctatt tttttgattt
    16621 ttatctccat attaatcctg ttgagtcctt tcttcaatct cccatcgtcc tcctaaagca
    16681 gtattcattc tgctgtggct ttaccaactg ctgttgtttt tgtgttaacc cagattagga
    16741 attctcgcct ttccgtctga gagcacgctc tccatgccat cctctttttc gagcaatata
    16801 cttattaatg ggtttcgtat aacattattt tatgatacca ttaaacaaaa gcattgagta
    16861 tatattcata cgatctgcct gtattaattt gagttttcat ttcaggaaaa agtggaggat
    16921 aatgggaaaa tgctgcagaa agtaggtgtg tttgaacctg cgtcaaaaga tgagctgctt
    16981 ggaagaattg agaatctccg acagctaatg gagaatgccg gcattgacta tgcattaatt
    17041 atacaaaacg tggattgttt ctatttctca ggaaccatgc aaaagggtat gctcgtaatt
    17101 ccgctcgacc aggaccccat gatctttgtt gagaagggtg ctgaacgggc agctatggaa
    17161 actcccctcg aacttacgcc tatcaaaaac gacaaagaaa taaaaaagat cctcagcgat
    17221 aagcatatcc tcaaaggtat ggcaggcctt gagtttgatg taatacctgt ttcagtattc
    17281 gaaagattga aacgggtcgt tggttttgat cggtgtaaag atgtatctga gttgatcaag
    17341 gaaacaagga ttatcaagag ttcttttgag cttgcccagg tacgcaaatc aggcgagatg
    17401 ctctcccgtg tttttgaaaa ggcacataac gttgtacagg aaggaaaaac agagctggag
    17461 attgaagcgg ctcttgttgc ggaaggaagg agaatagggc accagggttt tctcaggatg
    17521 cgagggctta accaggaaat gatggtgtca acggtccagg caggatacac aggtactatc
    17581 agtactatgg ttgacggcgc gataacagga actggagtga cacctgccat gccacagggg
    17641 tcttcattga aaaaggttga gcgaggagtc ccggtcacta ttgattacgg aggagcatac
    17701 aacggatata tcacggatga aacgagaagc tttgtggttg gtgagctcca agatgttttc
    17761 cgacgacctt atgagacagc acgatcgata attgaagatg taatgaccta tggcagagca
    17821 ggaataaact gtaccgaaat attttaccgg gcctacgaaa tagtgaagaa ggaaggcctc
    17881 gaggaatatt ttcttggtca cggagatggg aaggttgtat ttattggcca cggccttggc
    17941 ctcgagataa atgaactgcc ggtcataact gcgagacata gccggattct tcaggaagga
    18001 atggtctttg cctttgaacc gaagttcgtt ttaccacctt atggagccat aggaattgag
    18061 gttgatttta tcgtgcgggc agactgcctg gagcgggtga cagcaaactc cattgatatt
    18121 gtgacactat aacgtcctgt tcaaggattt gtctaaagtt atacaaaatt atcttcaaaa
    18181 aactaaacca attatctgta tttttcgata atctatgtat attattgaaa gcaagcacgg
    18241 actatgaggc tagtgttctc atgagaagat atggcaaatc aggagttaat gccgacagca
    18301 tggttaaaac cgagaaggat atgaagggga gaagactttc ctggatgaat aaaacaatac
    18361 gtaaacaaag aaacagtaaa ggaaaggata agattattct gaaagatagc ctgatcgggt
    18421 tgtataacga agaatgcttc aatgagtttc ttgatcttga gaaaagaaga tgcaaacggt
    18481 cagaagatcc ggcttttttg atgcttgcag acctttctgc ttttgtcaat gaatcagaac
    18541 ggcacgatgt agtgaagtcg atgatggagg tattctgtga ggcaacacgg gatacagacg
    18601 tcaaaggatg gcacatagac ggtctggtca tgggcatcct gtttactgaa attaccggcg
    18661 atgaagcaac ttcaagggtt gcgctaaaac atattgccag taagtgccta tggcggttga
    18721 agtacactct tggcgcagac aagttctcaa atattaggat aacttggcag tcactgaaaa
    18781 gtgaacatat gcttgagatt cataaggtag gcaatgttgg ctccgattga ggagtagtgt
    18841 ttaagcgatt aattcacatt gattaatcga tatctgtttc acagaatcgt tattaaaggt
    18901 cttcttctta tttaccttcg gtttttacgt ttctcgcgcg ttcagatact cctttatcat
    18961 tgacaagatg atttgccaat actactattt ccagaattgt tggtaattct cataatagga
    19021 aaggaaggaa tatgaagctt tcagaatttc ttacaagcat cgaaagaacg atgttgcttg
    19081 atggagcaat gggtacacaa ttggctgaag ccggtttaga tatgggaggt aaaaccaaca
    19141 tcacccatcc tgacgcagtg ttgtcaattc ataaacagta tgctgaatgt ggggtcgaca
    19201 tgatcattac aaatacgctg accatgaacc gtattaatat tgaattacac aatatgggtg
    19261 tcgatgtagc cgaggtaaat tctgcagggg tcaggctggc aagagcagcc atctgtgagg
    19321 gtcaatacgt cctgggagat atggggtcaa caggcaagat gttgaaaccc catggcgatc
    19381 tatcacctga cgatgcatat gctgcattta aggaacaagc tattattttg gcggaaggcg
    19441 gggtggacgg gattattgtc gaaaccatgt ttgatctggc agaagccatc tgttgtaccc
    19501 gggcatgcaa agatgctgtt gacctgccgg tcattgtttc catatccttt agcactatga
    19561 agagaggtgg caggacgata atgggaaact ctgctcagga ttgtgctaaa gcgctggtgg
    19621 aagcaggggc ctgtgttgtc ggagccaatt gcggtaatat tgatccgttt cagatgtctg
    19681 aaattgtttc cacgatgggt gaagttgtat cattgcctct tttagcccaa ccgaatgcgg
    19741 gcatcccacg gtttgttgag ggccgaacgg tttttgacat gtctccttct gattttgctg
    19801 aaggaataaa tcgctgtatt gaaaggggtg ccaggttagt gggagggtgc tgcgggacat
    19861 ctccggatca catcagggct attgtggaac gaataagagg ataggtgcac accgggcggg
    19921 tgttgcaaaa tataatcgac agaatatact tgtcaagatc cgtgggaaag ggcaatgttt
    19981 ttttgaatgg attagactat ggaaaaatca gtgaatacat ccgagatgga ggaaaaccca
    20041 atggatggaa aaagggtaag tgagagcgca gtacatacgg cccagctcat gtctccgcag
    20101 gacgcaaatg ctgcgggtaa tgttcatggg ggcgtaatca tgaagctcat cgatgatgct
    20161 ggcggtgctg tggcatatcg acatgctcga agcaatgttg tgacagtatc catagatcgt
    20221 ttgaattttc tcaatcctat ttttgtgggt gaccttgtca cgtttaaagc gagtataaac
    20281 atggccggca agagctccat ggatataggt gtacgggttg aggctgaaaa ccttataact
    20341 ggacaggtgc gccatgctgc atcagcttat ctgacatatg tggctctcga tgttgatgga
    20401 aagcctaaaa ccgtggctcc ggtgattttg gaaactgcag aagaggaacg tcgctttcgt
    20461 caggctcatg cccgaaagga aattcgcaga caggcacagg agagccatgc cagattggcg
    20521 cccgagaagt gatactgact taacaacagg tataaaactt ggatcacaat tcttgcagga
    20581 agaatgccct gactgcctct gattttccac atgtgcgagg tccttcagtg catttgcctt
    20641 ttacgcaggg gggacctgag tagcggaaaa tggtcggcgc aacaggtttc acaagtttca
    20701 gcatggcaag cgccatggcc ctgatttccc attgtgcccg gttacagagc cgctgccgaa
    20761 aaaaatggag aagttctctc gcattcattg tgatcatgat tttggtttct gtggcatttg
    20821 gcaatacaaa acgtgcatct tcccgggcag attcggttga atgacccagt tgggcgagtt
    20881 tgtctgcaaa gtatgaatag gattgttgca tttcctccat cattttttca aaatgcgcct
    20941 tcatttctcc gttctctttg attgaaggag gcatgatgta atcaaattcc ctctcgttca
    21001 cataccgttg tgactgttga ctgtatgatg cgaggcgatg acggacaagc tgatgagagc
    21061 aagcacggga tatgccttca acgacaaaag taaaggaagc atgctccaca gggctcaagt
    21121 gtccaaccgc tgtcagttta ccgaggaaat cctgctggtc gttatcaccg accttcgaga
    21181 gtatgtcctc agcgtctgcc ggactgtaac agagccgtgc cgcagcgctg attgtctttt
    21241 ccggttcagg gctgtagctt acaagcatta cttttagttg agcttccatt agttcatgcc
    21301 tttaatccct gatcatgcct ttcataagac gtcatatctc aatgcctctt ctcctcagct
    21361 cctctgtctt cttcttggag caggcttcgc agcggaactc cggatgagca tcttttgttt
    21421 tatcatattc cttggccttt atgatgcgat agccccgtgc ttccttcccg cagtccacac
    21481 acacaacgac atctcttatt tcccattgta cggaaagacg gtctgatgtg aagataaaca
    21541 tgtctaccca gaagaagatg agcccaccga taaggttggc tatcactgtg gctgtgatct
    21601 tatccatgta agataataca tagagaacgc cggcaaggat aggggttgag agttgccatc
    21661 tgatcagata caaaccgtat ctttttaaat ttactttagc cataaatgta tgtccccctc
    21721 tctttctcaa atcaggtaat gctattacct ttcaccattc gcgttttgca taccaccata
    21781 ctttggacct ccctttaatc ttcaatcgcg atcacgttcc attttttctg aatcaacagt
    21841 gctcatgagt ttaaaaaaag atctgcatgt tcccggaatg tcttcagaca tggtaatcga
    21901 ggtgaccacc gcaatgccat ttgcgccagc ttgaagaact gagcggcaat tattaagatt
    21961 gatgcctcct attccgacaa tgggcagaac ggtattgttt cttatcatag caacaccctc
    22021 aagtccgggg caatatccca gatcttcttt ggtttgtgtt ggaaaaacac cgtttactgc
    22081 aagataatcg gctccatccc tttctgcctg ttgagcctca cccactgtct taacagatat
    22141 gcctatgatc acatcattac caagtatggc cctggccttt gtaatgggca tgtcttcttg
    22201 gcccagatgg agtccgtctg cggctacttc tttcacaaca tcaacagcat cgttgataat
    22261 caggaatgca ttgtgtgtac gcgtaagttt catggcctct gatgcgagtt tggaatattc
    22321 aggcaaaggc agattttttt cacgaatctg gattattttt actccgcccc tgagggcaag
    22381 ttccacctgt tcaagaacag agtacccttt gtgaagaatg gggtctgtga cgaaatataa
    22441 gcgataatct tgtaatgatg gctttctcat aatgaggcct caaaacctat atcagcgaac
    22501 aggacgtatt cgtagttttt ccatcattgt tttttgggaa aggtcgtaca gagcatcgta
    22561 cagtgccacc tggaaactgc ctggcccgtt cgaaactttt gctgcttctt cgccggcaag
    22621 accataatac ccgagggcag cagcggtagc aacgagagga tcaggctcaa cggcacaaaa
    22681 acatgatatt gcagttgatg ccccacaccc tgttcctgta acacggcgga acatgggatg
    22741 tccattgtga atttcaattg atgaatggcc attggtaaca aaatcaattt ctccggtgac
    22801 agcgactacc tttctcaatt ctgcagccat cacagtagcg ccatgacgaa cagcctcaac
    22861 actttccatg gagtcaacac cacgaatttt tattttttcc cctccagcga atagcgacat
    22921 cacctctgat gcattaccgc ggacgactgt aatggggatt tcggagagta tcctcctggc
    22981 ggcttcagtc ctcagaggtg ttgcgccgga acctacagga tccagtacga tgggaatacc
    23041 ccgttctccc gcaaccttcc cggccaagat catggaatca agccagtacg gtgtaagagt
    23101 tcctatgttc agatttagga cactggtaaa ggctgacatg gcctccatct cttcgtgggc
    23161 atgcgccatt atgggagagg cgcctattgc aagggttaca tttgcagtac tgttcattac
    23221 aacaaagtta gttatgtggt gaaccagagg ttgatgcttc ctgatttcga ggaggatttc
    23281 tatggttttt tttgataatt ctttttcagt cattttatca tcccatcatt aacagtgtac
    23341 aatctccaat aatcagcaac ctttaatctt gtatctttcg atgttcactt tttgtttttt
    23401 cttccagcct ttcctctttt ctttcttcca tcttcctttt gaatctgttc ccttactgtg
    23461 ttgccgtatc ccgtatcccg cacaccggac tctcagtctt ccagtatgga gacatctccc
    23521 gcaatcgcgc tataattggt ggtagtttgt tgatgacaaa gtctatctcg tcctctgtat
    23581 tgaacacgct caggctgaat ctcactgaac cgtgagccat ggtaaaagga acgcccatgg
    23641 cacggagaac atgggagggc tgtaaagaac cagatgtaca tgcagatccg gacgatgcgc
    23701 agatgttgaa ctggtccatg aggaggagga tgccttcccc ctctataaat tcaaaactga
    23761 tattggttgt attgggcaaa cgattcgttc tgtcaccgtt tactttgctg ttaggaatgc
    23821 ttttcagaag ctcatcttca agtttgtccc gtaaatactt caccctggtg ttttctgttt
    23881 ccacattgcg ggcagctaac tggcaggcct ttcccagccc agcaatgctt gctgtgttct
    23941 cagtccctcc gcgacggccc ttttcctgat gtcctcccag cataaaaggc aggaaccgtg
    24001 tcccctttct gatataaaga acaccgattc cttttggtgc atgaagttta tgtccggaga
    24061 aggatagcat atctatgtta ttggctcgca tgttgatggg gatttttccc acagcctgaa
    24121 cagcatccgt gtgaaaaaga atccctctct catgggcaag ctgtgctgcc ctttcaacag
    24181 ggaaaatcac ccctgtttcg ttgtttgccc acataaggct caccactgca gtatccctcg
    24241 aaagcgagtt ttcaaaaagc tccatgtcga gccttccgtc ccgatctact ggcaattccg
    24301 ttacccggta acccctttgg gttaaatgct cacataatac ttttacagca gggtgctcta
    24361 cgcggcttgt tattatgtga cttttatcag gagctacaga aagagcagac atgatagcag
    24421 tgttgtcgct ttctgtgccg caactggtaa agatgatctc ctccgggtcc gctccaatta
    24481 agcttgctac attttctctg gcatgctgga tcgtttttga tacctgccct ccgaaggaat
    24541 gcatgctcga agggtttccg tagagttcac tgaaataggg gagcattgtc tccagcactt
    24601 ccggggcaac ctgtgttgtt gcattgttat ccagatagat tgtcttcatt ttggttcctc
    24661 ctctacaata aggccaggcg aaacgaattc tcgaagcttt tgttctatta agcccttcag
    24721 ggtgaattgt gaggccttac aggtagcgca cgccccgcgt gttgctacaa tgaccctgtt
    24781 gccaatgaca tcaatgaggt ccacatctcc tccatcctgt ttaagagacg gtctaatctc
    24841 tcgttcaatg gtctcctcta tcattcttat tttttgtatg ttggtcaact tcttttgttt
    24901 aggctctttt aacccctctg gttctgacgt aatattaact ctgtcaatga ttgcctggat
    24961 tttttcgtga caatttgtac agccaccgcc tgccttggta tagttggtga cttcttcaac
    25021 agtagttaaa tgattttctc tgacagcttt ttcaatttca cggtcagtga cgccgaagca
    25081 ttcacagaca atctgttcac caggttcttt tgccggtgct ccttgacagt ttgctatggc
    25141 ctgttccagt gcctgttgcc ccaggactga acagtgcatc ttctcctcag gcaggcctcc
    25201 aaggtaatca gcgatgtcct tgtttgttac cttgatagcc tcctcaaggg tcattccttt
    25261 tacgagctca gtgagggcgg aagcggatgc gatggcactc gcgcaaccga atgtcttgaa
    25321 cttcgcctcc ttgatccttt tgttttcatc aagtttgaaa gtaaatttga gggcgtctcc
    25381 acaggcaatc gaaccaacct cggcaacacc atctggattc tctatctccc ccacattgcg
    25441 gggattaatg aaatgttctt taactttatc tgtatattcc cacatttgcg atctccttat
    25501 cgattatttt taaagcaacc attcggtctg tgtaatgcct tactcagggt tggtgcttat
    25561 tgtatcatat tactgtacaa tgcggtagat acttgcggtg gtccagaacg attcattgta
    25621 ggttcaagac tttcgcccct ctgattttcc tggtcgtgag ttcaagaagt gccctgttcg
    25681 cgtcttccag cctgaaaacc tgtacttccg gccgtatcgg tattgcagcg gcaagttcga
    25741 gaaagtcacg tacatcctgg cgggccacat tagcgacact cttaatctct ttctcatgcc
    25801 agaggtggat agagtaatca aggttgagca actgctcctt atctatatct tcttttcgga
    25861 tggcattgat gacaagcctg ccgccgggtg caatgtttgc cagtgtttta acaatcggct
    25921 tccacacagg ggtcgtgtcg atggcacaat tcagtttcag tgggggagtt tctccgatat
    25981 ctccggccca tcctgcgccc agttcacggg caaatgctct ctcactttca ttccgtgcaa
    26041 aaacgaatat cagtgagttt ggataattgt ggcgtgccat ctggataacg agatgagcag
    26101 atgcgccaaa cccgacgagt cccagtatat caccatcctt catgccggtt agcctgaggg
    26161 atcggtagcc gattgttcct gcacagagta aaggtgctgc ttccaggtca gagaaaactc
    26221 ctgggatggg aaaggcgtaa tcttctgaaa tagtcgtata ttctgcatac ccaccatggg
    26281 cgtctcggcc tgaagcttca aatttatcac acaggttgtc gttaccttcg cgacagtgca
    26341 tgcacgttcc gcatgccgag tgaatccaag caatgccaac tctgtcaccg atcttgaatt
    26401 tctttacatc cttccctgtc acttcaacac gacctatcac ttgatgacca agaataattg
    26461 gaaagaaggg tggtggagtt ctgccttcta tctcatcaag ctcagtgtga cataccccac
    26521 acgcagaaac cttaacaagt aggtccttat cctctggaaa aggcttcggg aaatccacca
    26581 attcaagggg ggcattgttt tcccgcaggt tagtaatttt gttgagaatc atagccttca
    26641 ttaccttcct ccgttctttt atctggctat aagtttttag ctttcagcgt ttggcaagcc
    26701 catgatgcag aactgttggt tgtaaacaat ttttttccaa catttcactt ctttctttta
    26761 tttccttttc ctcttttact atggctgtga aattatagct acaagctttt tgccgaatga
    26821 tccaagcagg gttttgtgcc tgttcgttta ataaatctct gaattgcccc aaaatactca
    26881 tcgaatcctg aaaacattat gtcgttatga tcggctccgg gaatgataat aagttccttg
    26941 tctgttgctc cgatgttagc ataaagatct tctgcctctc gaagtggtac aagtatgtcg
    27001 tattcaccgt ggatgataag ggtggggacg gtgattttcc tgaccagtga gatgcattca
    27061 tcataaaccg tggacaggtc tattgagcct gcaggcagcc caagatgcaa aatgagctgt
    27121 gtgatgctcg gaaacccact ctcaataatg agtcctctga tctggtcgga atagtgatgt
    27181 ccaagttcca gcgcagagat gcttccaagg gaccgcccca taacccagat gttattttca
    27241 tggctgagtt gtaaaacgtc ctcttttatg tccctgaaaa ggatataggc gtcgtgggca
    27301 agatagttaa gtgcagggtt gcctgtactt gcaccataac cgcggtaatc cacaacaacg
    27361 agattgagtt cacgtttatt atagaaaggg gctatgttat catagtcgct gaccacctcc
    27421 ccgttaccgt gaaaaaaaac aatccatggc caatcatcgt tgcccttgta aaagcgacag
    27481 ataatcacca cgttcccatc gactgcgatt gtcttgtcaa atgcatttac aggtggcttg
    27541 ttgtgagtgc tgcgaggata aaagatgtag tttaaggctg gtgaattgtc gatttccctg
    27601 taatcgggca cggatacctc catactgtgt tatgaagaca tattacagaa aaacacagaa
    27661 ggattcaaga ctgtgaataa taggaaaagc cgtttccaaa ggatgatcag acccaaggtt
    27721 acgtatatcc gagatctttg gagatagaac gggaggcttc aaggagttgc ctgattgtgt
    27781 tttcgatagt tccatctttt atttggaaaa ccggctgggt tgcgccaaga gcagcgacta
    27841 cgttgccaga gaaatccctg atgggtacgc cgacaccgag gagcccttcg acgatctcat
    27901 cctgttctac gtagtagccc tgctttttag ccttttcaag attctccagt atctgatcca
    27961 tgttcgtgat tgttttggga gtaatctttg ttggtgggta taaattaagc aggcggttta
    28021 tctccttgct atccatatgg gcgaggagga tcatgccaag catgccaaag taaggaggtc
    28081 ttttaaggcc tatgtgggag gttacccgaa taatgctgtc catttctctt ttgtcaatgt
    28141 aaaaaagctg atcatccttc atgacaccga ggaggatcgt ggccttaagg ctgtttgaaa
    28201 gggcatccat atacggagct gccgcttttc tgactgagag agacgagtat acaagtcctc
    28261 caagttcaag aaagcgcagc cccaggctgt atgtttcctg atcagggtca tacttgaaga
    28321 aacctgcccg ttcaaatgtc ttaagtattc tgaatacggt agttttgggg agccctgttt
    28381 tcttctttat ttcggagagg gtgaaagagt catgaccata ggaaaagata tcaaaaatct
    28441 ttatgctctt ctcaaggact ctaacggaat attttttttg atcatcattc ataatttaaa
    28501 aattaaaggc ataaggcaac aagtgtgtca ataacagatt taaaagcttg gttgcagccc
    28561 ttaccccagt tctttctcac atacttaaaa ggccgttccc caaattatgg aggagcggcc
    28621 atgacaaaag gggagagatg ggggcgatct ccccgaaggc tatgccagtt gaaggtctaa
    28681 acaaatctaa ggaccgataa cactgacgta taaccctcaa tcggttgctg gggttatgaa
    28741 tttaagtata taaagaaaag tcagtattat ttcggcgttc ttccttatag cattattatc
    28801 ttttgcaaga gtttttaact tttccgcgct cttttctata ttcattattt catttctgat
    28861 ctcatccatt agtgtttacc tctcatatct ttaaatcttt ttgccttaag gtcgtaccgt
    28921 ggtagtgtcc cctgaggttc gacaacaacc ttacatgaaa gatttgtttt gatcttgtgc
    28981 ctctcgccaa gcctttttgc aacttccccg gcctcatctt tattcagttt cggatcggcc
    29041 tctatcctga gcgttatctt gtccataatg tcttcacgag tgacaacgag ttcgtactcc
    29101 actatctctg ggaattcaga cctgataagc tgttccaccg tctttggcga gaaaaggaca
    29161 ccacgaatct tgctgatgtc atccaccctt cccacgatct ccatgagctt gagcgatgtt
    29221 ctgccacatt cacaggttga agggcccttt atagcaacat catttgtgtt gaagcggatc
    29281 acagggaacg atcttcttcc aagaggcgtg ataactatga caccaagttc accctcagac
    29341 acctctttgg acagagtgtc tttatcgagc aactcaacga ggaagaaggg ctcaacgata
    29401 tggaggccct gctgagcctc gcacatgcct gcaaaaccac aggtttctgt gctgccgatg
    29461 tggtcataca tcttgcaatt ataggtttct tcaatcctct tgcgtgttgc ttctggaagc
    29521 ggttcccctg caccaagcgc acgttcgatg ccgagagact ttggatcaat gcccatggat
    29581 gcggcctcct gagcaatgtt gagaagatag gttggtgtac cgcacatagc gtttgccttg
    29641 agctcgatta ttttattaac acgtcctttc gtatcaagtg ctccgcccgg cacaacttca
    29701 cagccaagtt tttctgcagc atagtgggct tcccagaatg ccacataaac attgtaacca
    29761 aagggaagaa aaactctgtg ccgtggtctg aatccagcca tgtaaagaat atgacaccac
    29821 gattctactc tccactgcca gctttcgtat gattcaggca catagacggg ctttcctgta
    29881 gtaccgctcg tctgcctgaa acttgaaaca ttagcaatat caatgccaag aagaccgccg
    29941 tatggaaagg ggtccacatc ttcctgccac ttcctcagtt cttccttgta agtgaaggga
    30001 attttcttga tatcctcaaa aactttgata tcatcaatat tgatcccttt cagtttatcc
    30061 ttgtaaagcc gggaattggc tttggcgtat gagagaaggt tttggaaata ggagaactct
    30121 attttctcca gcttctccct cggcaaagtc tctgtataag gattccagaa ttccataacc
    30181 actcctttca tttcaaatta tgaaacatcg tttctaaatt gcatagaaca tgatatgaga
    30241 aaccgaatca tctgtcaagc aaattcagag gaaacatgaa tggcaaagga aagttttatt
    30301 caagccgctt tttgatatct tccctgagag ccttcttatc taccttcccg acttttgttg
    30361 ctggcaaaga ttcaatgaac tcaatccttt ccggtagctg ctgtacagaa gccccatttg
    30421 ccttaagaaa ggctatgatg ctgtcaaacg tgggtttttc tcctgaacgg agaacaacat
    30481 acgcacaaat cctttcacca aggatcttgt ctggcatacc cacaaccgct gcttcctgga
    30541 tttgaggatg ggcactgagg cggtgctcaa tctcaagggc gctgatcttc tcaccacccc
    30601 tgttgattat atctttgatt ctgcctgtta tcgttatgtt tccaaactca tcttttcggg
    30661 ccttgtcacc ggtcttaaaa aaaccgtcga ctgtgaaggt ttctttgttc tcctcctggg
    30721 atttaaagta tcctgtgaaa attcccgggc ccttagatac gagttctcct tcaacgccgg
    30781 gaggaacctc aattccacta tcatctataa ttttgattat atcgtgtgga caggcctttc
    30841 ttcccacact gttgcagata atatccatgc tatcccctgg tcgggttgcc gtgttcgtac
    30901 cctctgatga gccgaatcca tttatgacga tacatttgag tttttcatag gcgccgcgca
    30961 acagttcagg tgtgcttggt gccccgccaa cggatatctt tttcagggat gacaagtcgt
    31021 atctttcgat atcctccatg ttaatgattc tggcaatgag tgctggcact gacggaatgg
    31081 cagtaacctt ttctttttgg atccattcac aaatatcctc cggttgggtt gaatcaagga
    31141 ggacaagttt tgaaaaattg agaaatgctg caccaatacc ccagtgcatg gccatgctgt
    31201 gaccgacagg ggtgactacc ataagcgtgt cattgctagt tatttcccaa acccgtgaat
    31261 ggtactctac gttgcagatg taacagttgt gggttctcac actcgctttt ggcaggccgg
    31321 ttgttccgcc tgttggcatt atatgggata cctcatcagg gtcaggccgc ctttcctcaa
    31381 gggctttgag gttttcaggg gtaagttctg cctcttcgat caattcatca agggaagggt
    31441 atcttccttt tttcccagtc ctcacctgaa taatggtttt aatttgaggg ttttctttca
    31501 aaacatcatc gatgatgggc tcatagtcga ttttaccgta ctgttttggt actatccatg
    31561 ctactggctt tgtcaaagca cagagatgat tgatctctga ctggttgtgt ctgggtataa
    31621 ggagaagtgt caaagcacct atcttttgca tggcaaagaa agaaatgatg tattcatgcc
    31681 agtttggaaa ctgaagtaat acccagtcct ttggcttaat accacgcttc atgaggctta
    31741 tcgccaagcg atccaccttc tcccgaagct gcccgtaggt ccatctgcca gtgccgtcaa
    31801 ccaggccaat cttgtccgga tagatgtcgg tagccttgtc aaagaggtcc ccccatgtca
    31861 ggccgagcca ccatctcagc ctgttgtact tttcacggtc ttcctttgcg tattctgtaa
    31921 aaccttctat cgccatatgt cacctcatat ttatattttt tataaaaaag caacagcagc
    31981 aggaatatgt tagatccgta ctattatgta ttctgtgctc agtaaagaat tatcacactt
    32041 acatgcaaac taaggatcgc ttttttgtgg acaggctgta cattccatct aatgaaaatg
    32101 ctgttcatgt gatgatatta ccatgagggt tatatttgtc aagaaaattt tatctttttt
    32161 tcttgacaaa cagaatcgtg tgctgttaac tctatgaaaa ggtgtttcat taaatgaaat
    32221 cgaatggaga ggatacaggc aagagctttt ataacaggtc attagagaga gcattacaaa
    32281 tactgaatgc tctgagtgac gagaggccaa ccctttctct ggcgcagctt tcggagaccc
    32341 ttaatctttc gagagctacc gtgttaaggc tctgctcaac attgttggat ttcgggtttc
    32401 taagacagga tcctatgtcc aaacattatt ctctgggact tagaatactg gaccttgcta
    32461 aaagcgtttc cgattccttt tctctgacga aaattgcatc ttctcatctg aaaatccttc
    32521 aggtaaaatt aggtaaaact gtattcctca gtgttctgga tcaggacgag gttctttaca
    32581 tagacaaact tgacgatccg aagaacccaa taagctttac ttcaaagatt ggtacgcgcc
    32641 gccctccgta ctatggtatg atgggatcaa ttcttatggc atacctccct cacggagaaa
    32701 taaaaagaat cctccataag cagccattga aagcatttac tcaaaaatct attacgaaaa
    32761 aacaagagtt tttaaagtgg ctcggtaagg tccggttaca gggctacgtg atcgatacag
    32821 aaacagcgct tgagggaata tcaggtgtcg cggcccctgt ctttgactat accggtaaag
    32881 tcgtggctgc cgttggggtt ggctttatct cttcatctgt gaaggaagag gagatggcta
    32941 aaatagtcga ggagacaatc aggacaacct cggctatctc taaagagctc ggctataaag
    33001 agaagaaaaa tgaatcaaca taggcaaata gtcttctgca gaattaaaac gccagttcaa
    33061 aaacaaattt atagactt
//
LOCUS       DAOOMC010000013        33057 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdus sp. isolate AMR_MDS_2685
            NODE_1114_length_33057_cov_6.551542, whole genome shotgun sequence.
ACCESSION   DAOOMC010000013 DAOOMC010000000
VERSION     DAOOMC010000013.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864760
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdus sp. (wastewater metagenome)
  ORGANISM  Syntrophorhabdus sp.
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae; Syntrophorhabdus.
REFERENCE   1  (bases 1 to 33057)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 33057)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,729
            Genes (coding)                    :: 2,708
            CDSs (with protein)               :: 2,708
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 7 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33057
                     /organism="Syntrophorhabdus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1114_length_33057_cov_6.551542"
                     /isolate="AMR_MDS_2685"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2699756"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(133..1545)
                     /locus_tag="PK125_02345"
     CDS             complement(133..1545)
                     /locus_tag="PK125_02345"
                     /inference="COORDINATES: protein motif:HMM:NF014910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HPB36982.1"
                     /translation="MIKESIIVDLQRIVGKENVFTSPECLKTYSYDGTTTWTHKPDVV
                     IFPLNTKALSQVMKLSNREKIAVTPRGGGTNVSGGSIPISGGIVLCLSKMDRITKIDK
                     ENMMATVEAGVVLQDLVLELAKEGLFFPPDPQSYYGATIGGIIAENAGGPSCLKYGVT
                     KQYVLGMEVVLPTGEIINLGGRTLNNVIGYDLLHIIISSEGTLGVVTRADLRLMLLPP
                     ARKTVMAVYGDAVIAGENVSRVLEAGVIPTKIEYIDNWVINKIEETMPFGLPRDADAV
                     LLFETDGASEAVERETEIIVEVARKHGAKEVRIAESINEANQFWMARRVGASAIYRDT
                     KTVLVEDVTVPRGNLPVLIKRCKELAKKYNLEIVALGHAGDGNLHPAILTDITDVNHY
                     QKATEAMDEIFQAAVEFGGVISGEHGIGLEKQKFLMKTIDPTVLTIMKKIKTLLDPNN
                     IMNPGKIWSESDKPPRKFEI"
     gene            complement(1587..2870)
                     /gene="larA"
                     /locus_tag="PK125_02350"
     CDS             complement(1587..2870)
                     /gene="larA"
                     /locus_tag="PK125_02350"
                     /inference="COORDINATES: protein motif:HMM:NF033504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050043 - lactate racemase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-dependent lactate racemase"
                     /protein_id="HPB36983.1"
                     /translation="MDYYLNYEGGRINFSLPSGWNVLTCQDCSAVSEVQDVDREIERV
                     LDSPIGSLRLEELGKPGMDVVVLFDDIQRPTPSHLAFPHILNRLNRAGVPDERISAVC
                     ALGTHPLHTREQLEKKAGKETFRRLEGRVYNHDPRSNENILIGRTSKGTLVEVNKLVA
                     QSDLIIGIGECMPHPYAGFGGGCKIVMPGVASYRAVADHHYTWMRHRNCKLNLLEGNP
                     FYEDIVDAGRLSRLSFKLDFVMNERHGVIKAFAGDPVEAHKKASEFASSLYLIPLAKR
                     PDVVITSAAPLEIGVNAMKSLLNASFAVRTGGTIIWVASQKNSGPIMPLVEQMATKET
                     ANEYHHRLIRGDIPDHLKNFGISFIMMVVIFKELSEKLSVVHVTEALTKEQVDMMNFI
                     YASNLDDAVSATFKSMPKADVAIFPSGGSVIPGIA"
     gene            complement(2912..3790)
                     /locus_tag="PK125_02355"
     CDS             complement(2912..3790)
                     /locus_tag="PK125_02355"
                     /inference="COORDINATES: protein motif:HMM:NF014794.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HPB36984.1"
                     /translation="MENEKVIVDMMRSGHMACPGCGVVIAMRLVLRALGKKTVAVIIP
                     SCSSIIASTYPNTSLKVPAFHGTFESAAPTAAGISYVLKLQGKDDISVVAFAGDGGTF
                     DIGLQALSGTVDRNEHYIYVCLDNEAYMNTGIQASSATPECAWTITTPSGRPFRKKNI
                     MEIVAAHRIPYAATASIGYPQDLMDKVKKARNIQGTKFLHILTPCATGWRMAEGLTVK
                     AAMLSVETRLFPLYEVFDGRRYVITYEPQGFPIDEYLQMQGRYGHLTQDQIANMQTQI
                     EVEWENLRNKVKDCQC"
     gene            complement(3792..4946)
                     /locus_tag="PK125_02360"
     CDS             complement(3792..4946)
                     /locus_tag="PK125_02360"
                     /inference="COORDINATES: protein motif:HMM:NF013974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB36985.1"
                     /translation="MRELLTGNYAIAEAVRLAKAQLIPAYPITPQTPIYEKLSEMEAQ
                     GTLPGIMVRVESEHSAMAMCISASLTGARVFTATSSQGLALMHEMLHYASGNRVPVVM
                     GCVNRVLAVPWSFGSDQTDTLAQRDTGWMQFYCEDNQEAFDTVIQAYKISEIVMLPSM
                     VIIDAFFSSHFIEPMEVPDQVAVDRFLPKAALPERFDIENPTFVSNVVTTGSQFMPFR
                     KRGFDDMENAKTVIKKVDKEFEEHFGRRYGMVEAVQTEDAQLVLVTSGSMTSTARVAV
                     DSLRNKGLKIGLLKIKTFRPFPSGEIKSALQGVAKVAVVDRNISLGNEGIFCQELKSS
                     LYNSDIRPPVYGFIAGLDGLDVSPDTLEGIAMEVMGKDEPNDLPVWIGGS"
     assembly_gap    5066..5165
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<5166..6530)
                     /locus_tag="PK125_02365"
     CDS             complement(<5166..6530)
                     /locus_tag="PK125_02365"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HPB36986.1"
                     /translation="MIRKENIKLVDHETIIPLSRLSTEIFKTGQWSPRKPLYREKLSP
                     CREACPVGNDIPGILSCVAKGDFDGALALILHENPLPGICGRVCYHPCQVNCNRIQFD
                     KAVEIRSLERFIADYGQATPQVWTNQKGNQVKVAVIGSGPAGLSAAYFLALFGHKVTI
                     FEARPEPGGVLRYGIPEYRLPREIVKREIDRILGLGIDLVAGERVDTEKLTDIKRKYD
                     YVFLGTGAWVPQRIEASFEHRDTIIYGLDFLSHGDKTGMCEGKKNVVVVGGGDVAVDV
                     ARTARRLCDPDAGITMVAPEKEGEFPAIQEGLVEAEQEGIHMIGEYQPIEFIGKEHLK
                     QIKFIKTKVEKDQATGRYRLSPVKGKDRFLDADLAIIAIGQIPDTSVFFESIVDKESS
                     RVFVDELGSTPLSGFYAGGDVIRQRPAVADAILSGKRAALSIHLEVNGYEPNRVMTSL
                     KLGKG"
     gene            complement(7073..7759)
                     /locus_tag="PK125_02370"
     CDS             complement(7073..7759)
                     /locus_tag="PK125_02370"
                     /inference="COORDINATES: protein motif:HMM:NF019349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HPB36987.1"
                     /translation="MMTLDFTNDTAIWVKVYEYVKEKIISGEIHPNQRLVEAKVAKEI
                     GTSRTPVREAFHNLQKDGFVEAIPRVGYKVKSISKEEVIQVCKIREALETLAAEWAME
                     KAYPVLIQELGKIISESEDKISKQDVHSYPDLDARFHETIARLSGSTYIMEITQLMRR
                     HMVRHRIASFYHSGVFLRALDGHRLIYKAIERRDCDEVARAIHYHLELSLRDILMAAP
                     KEELSETDRI"
     gene            complement(8319..9236)
                     /locus_tag="PK125_02375"
     CDS             complement(8319..9236)
                     /locus_tag="PK125_02375"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: protein motif:HMM:NF014296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0006555 - methionine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HPB36988.1"
                     /translation="MSTQSNLERVLKSGTFAVTSECGPPRGADPEVVKKKGSYLLGYV
                     DCVNVTDNQTSVVRMSSFSACLVLKGVGLDPIMQMVCRDRNRIAMQSDILGAAALGIN
                     NLLCLSGDHQKFGDHPTAKNVFDIDSIQLIQTVKGMRDEGRFISGQEVKGKPNIFIGC
                     VENPFADPFEIRALRLAKKAAAGAQFVQTQCVFNVEKFEKWMEMVRDLGIHEKIHILA
                     GITPMKSIGMAQYMKNSVPGMDVPEEQIERLKGVPKEKVTEEGIKIAVETIERVRAIK
                     GVAGVHIMAIEWEEKVQEIVKAAGLHPRP"
     gene            complement(9233..9901)
                     /locus_tag="PK125_02380"
     CDS             complement(9233..9901)
                     /locus_tag="PK125_02380"
                     /inference="COORDINATES: protein motif:HMM:NF023646.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase C-terminal
                     domain-containing protein"
                     /protein_id="HPB36989.1"
                     /translation="MIVANRKPFEEIIEMLKPYSKILLLGCNECVTVCATGGSKEVAV
                     LASEIRLYRQKEGKPVEIGEVTLERNCDPEYVESLKPFIHDYEVVLSMACGCGIQFVG
                     EHYRDRLVLPAVNTTFYGVTEEHGVWTERCQGCGNCILHKTLGICPVTRCAKSIFNGP
                     CGGSQGGKCEVDRDIPCAWHLIVDRYRELNMMDKYLEIQPMKDWSTSRDGGPRKRVRE
                     DLKI"
     gene            complement(9991..10407)
                     /locus_tag="PK125_02385"
     CDS             complement(9991..10407)
                     /locus_tag="PK125_02385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013822575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(10360..10362),aa:Sec)
                     /transl_table=11
                     /product="hydrogenase iron-sulfur subunit"
                     /protein_id="HPB36990.1"
                     /translation="MGKFEPQIIAYCCEYUGYTAADLAGSMRLSYPTNVKIIKVPCTG
                     RVDITHVLKAFEDGADGVYLVGCLEGDCHYLVGNIRAKKRVEYLKHILDECGVGGERV
                     AMYNMSSAEGQRFAEVAREMTEKIRTLGPNPAKKEK"
     gene            complement(10509..14951)
                     /locus_tag="PK125_02390"
     CDS             complement(10509..14951)
                     /locus_tag="PK125_02390"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HPB36991.1"
                     /translation="MEKVDKVGKVLVIGGGIGGMEASLNLVEAGFRVYLIDEKPNIGG
                     RMAQLDKTFPTNDCSMCIMAPKLVEVGRNPNIELMMNTDVIDLTGEPGNFTVTLRRKP
                     RRVLPEKCTSCALCAPACPLEVGSDYNVGMSQRSAAFINFPQAIPSTYMIDREIAPCV
                     DRCPINLNARDYVGLIAEGRFLEALDIIRERLPFPSIIGRICAHPCEEECLRGRKVDQ
                     PIAICALKRFVADYEVGKREIPIPGVGPDKGKSVAIVGGGPAGLTCAIEVRKAGYKVT
                     IFETYDKLGGMLYVGIPPYRLPKDELAREVAIVEKMGIEIRLNTKVGRDISIKDIQNS
                     FDAAFIAVGAHLGRSLGLANEDAQGVLSGLDFLKSVIIGSPVEVGKRVFIIGGGNVAI
                     DAALTSRRLGAREVHVVCLEKWDEIPAHKWEIGQAVEEGIIFHPSWGPLRFDVSHGHI
                     KGIEFKRCTSVFDEYGRFNPQYDEETTIYHEADTAIIAIGQAMDTAFLSELDDLEVFH
                     DGRIKADPVTGETTVKGIFSGGDVVTGPKMAIDAIAQGKDAAESIIRYLEGRNLREGR
                     RAIETKLVEDVPTFMEKKARVAIQSLPVAERTGFQEVYQTLTEEEAKREAERCLNCRR
                     CLGCRICEEFCKPEAIDYFETPKEERIQVGSIIVTSGFDPYDATQKKEFGYGVYPNVI
                     TSLEFERILSATGPTASVVMRPSDGKIPKKIAFVQCVGSRDKVHEYCSSVCCMYATKE
                     AIIAKEHQNDMETTIFYLDIRVFGKGFDQYYERAVSEYGVRYVKSFVSRILEDPVTKD
                     VKIVYVDESGNLRSEQFDLIVLSVGTRPSEHLPTLANVLGIQLNEYGFVKTSQKNPLI
                     TSREGIYVSGACEAPKDIPETVIQASGAACEAGSVISEVRGKDLVIKELPPEKDVDAE
                     DPRIGVFVCNCGINIGGVVNVPEVQSYAKGLPNVVFSEENLFTCSQDTQDKMKQLIED
                     YKLNRVVVASCSPRTHEPLFRATIRDAGLNKYLFEMANIRDQCSWVHMHEKAGATEKA
                     KALVRMAVMNANNIKPLKDIMLDVNKKALVVGGGMAGMTAALKLANQNFEVFLIEQED
                     SLGGNLKHIFSTIDGLDVRSFMKETIERVDSHPLIHVETEAQIIDHTGFRGNFETGIE
                     AGPLKKYKKINHGIIVLATGGEEYKPNGMFLYGENEKVLTQTELESQIISGRLPRYER
                     VTMIQCVGSRNEARPYCSRICCSTAIKNAITLKEQKPEADVVILFRDIMTYGFLEKYY
                     LKARRLGIRFVRFEKANPPIVNNGNGVLAITCFDPSVTEEVSFTTDLLVLSCAIVPRD
                     NEAIATLFKVPRTIDGFFLEAHMKLRPVDFPSDGMFLAGLCHSPKNIRESIAQAEAAV
                     ARGITILSKDQIAAGGVVARVEPEKCAACLTCVRVCPYSVPVINEKGDAEIDISKCKG
                     CGICVAECPAGAIDLMHYRDAQIIGKAKSLFSEVTGDMVAECES"
     gene            complement(14993..15349)
                     /locus_tag="PK125_02395"
     CDS             complement(14993..15349)
                     /locus_tag="PK125_02395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB36992.1"
                     /translation="MDIDEKLDYVKTHYPGGAEKLTRLLNKKDALMSGNVYGEKMTGR
                     QFSLVFTSLLEAAFEKARILETLAKNDSTIDDLSVATGMRLQQVFDHMKDLLGKNMVE
                     ISGYAGREAVFKKVRR"
     gene            complement(15364..16227)
                     /locus_tag="PK125_02400"
     CDS             complement(15364..16227)
                     /locus_tag="PK125_02400"
                     /inference="COORDINATES: protein motif:HMM:NF014774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heterodisulfide reductase-related iron-sulfur
                     binding cluster"
                     /protein_id="HPB36993.1"
                     /translation="MTGLTYYPGCSLKTSSKFYETSIKQVFSFYGIEIQELPDWSCCG
                     ASAANTVDERLAHALAARNLALAEKQNNHLFAPCSACYIRTKVTNERMRQDKNFREDI
                     NSVISPLKCLGTGEVKNIIEVFHDRVGIEVIAGRLKYDISPLRVVPYYGCLLTRVMGM
                     EAFDDKENPTSMDSLLWALGVELVNWPYKMECCGASKTLTDKDITYDLSDKIMDMAVL
                     LGADVIVTPCPLCQLNLDLLPYLGRTDQALPILFLSEVFELAISGRLSGSNSHVIAVD
                     QAVKKVKKISG"
     gene            complement(16277..16801)
                     /locus_tag="PK125_02405"
     CDS             complement(16277..16801)
                     /locus_tag="PK125_02405"
                     /inference="COORDINATES: protein motif:HMM:NF024924.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HPB36994.1"
                     /translation="MNDLTFLKEMEALSGQNISACYQCYRCTAGCPVVTQMDIFPHGI
                     IRYIILGEREKVLKAKAIWTCVQCITCSVRCPNDIDIAHVFDTIRKVAIKEQKAAETD
                     TWTFDKIFLDSVARHGRLHEIEAILRYKVVKKNLFEDAKMGIGMLLKGRMGIFPHSVR
                     DREQLKEIFKKCNQ"
     gene            complement(17038..18030)
                     /locus_tag="PK125_02410"
     CDS             complement(17038..18030)
                     /locus_tag="PK125_02410"
                     /inference="COORDINATES: protein motif:HMM:NF014097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NrpR regulatory domain-containing protein"
                     /protein_id="HPB36995.1"
                     /translation="MNRTMFTILRVLDKYGDIVGSIELSRELRSHGIELSERTVRHYL
                     KLLDQMGMTKVQGKRGRMITEKGKSEVTKAFVTERINFVISKIDSLSYLTDFNVDTLE
                     GNIILNISFFSEKKLKDVVRIAAPVFASPYVMSNRIILVKGGDRIGDVQVPRGCMGIG
                     TVCSITLNGIFLKSGLPVTSRYGGVVEVEGGVPKRFTSLISYDGSSMDPLEILIKSGA
                     TDVAGAVAGNNGRILASMREIPVASIREAEVLKEKMSHKGLGGSILFGKPNQPMLDVP
                     VGIDKVGMLVVGGLNPIAAVEEAGISTESKAMSTLCEYSHLLAFNDAVSNLLYQ"
     gene            complement(18030..19385)
                     /gene="gdhA"
                     /locus_tag="PK125_02415"
     CDS             complement(18030..19385)
                     /gene="gdhA"
                     /locus_tag="PK125_02415"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HPB36996.1"
                     /translation="MSLIEEIIAQVKHRDPEEPEFLQAVVEVMESLEIVAERHPEFVK
                     AGIFQRIVEPDRTIVFRVPWVDDRGEVRVNRGFRIQFNNAIGPYKGGIRFHPSVNLSI
                     MKFLGFEQIFKNSLTTLPMGGAKGGSDFDPKGKSENEVMRFCHAFTAELFRHIGPETD
                     VPAGDIGVGGREIGYMFGYYKKIMNNHTGVFTGKGLDYGGSLIRPEATGYGAIYYAAE
                     MLATRGLSFTGKTVAISGSGNVAQFAMEKVNQLGGRVITLCDSNATIVDEEGICGDKC
                     DFVMELKNVKRGRIKEYADKYGSVCYLGDSVWDVITQQGLKVDVAIPCATQNEIDGKN
                     AEALVKNGCICVAEGANMPSTPEAVRIFHENNVLFGPGKAANAGGVAVSGLEMSQNSL
                     KMSWTRQEVDDLLLKIMKGIHKATLEASEEYGRKGDYVTGANVAGFMKVARAMLAYGV
                     V"
     gene            complement(19816..20511)
                     /locus_tag="PK125_02420"
     CDS             complement(19816..20511)
                     /locus_tag="PK125_02420"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HPB36997.1"
                     /translation="MTEKKDKTMMKEGEFSGQTYKIFIVDDHPIVRKGLSQLIDQEAD
                     LLTCGEASDAQSALQILKKLKPDLTIVDISLQGMDGIELTKNIRARYGNLPVLIISMH
                     DESLYAERALRAGARGYIMKQEATEKMMEAIRRVIRGDLYISEKVSAGIVKKFVDGKS
                     KRSSSPEELLSDRELEVFRLVGQGFGTRQIAEELRVSVKTVETYRANIKEKLNLKNAR
                     ELIKHAVYWLESL"
     gene            complement(20518..22062)
                     /locus_tag="PK125_02425"
     CDS             complement(20518..22062)
                     /locus_tag="PK125_02425"
                     /inference="COORDINATES: protein motif:HMM:NF019350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HPB36998.1"
                     /translation="MECCTLSAHLVDASEKMDAHSQNDKLNNSDELAKLRKEVACLRA
                     SEKEHRRTRESLQNKTRALGERVKELNCIYALSSLVEKHRTSLERILHHMVEVMPSAW
                     QYPEIAAAKVAMGANTFITANYRPSKWTQSADIRVRGEKSGFLQVIYLEERPQCWEGP
                     FLKEERKLINSLAKRIGEIAERKLAEDDLKESMVRNKALQNSIPDLIFRIKKDGTILD
                     FKKGKTLGQKIKAKKIIGRKIADLAHEYPFLSKDITGRGMTNIEQVFEKGTTQLFQLQ
                     YRHGSKTYCYEVLLTVSGRDEVLGIIRDMTEWRKLEKQVLEISEWEKQRIGQDLHDSL
                     SQQLAGIAYLGKTLERKIAAKSFEEAKDANQIVKLIDDAIIQTKGLARGLYPQWLVGE
                     GLMAAFSELADTVEKLFMIKCCVEYDQLVPIDDDLTAMHLYRIAQEAVNNAIKHSLAR
                     NITISFRNDKNQTVLTIRNDGIGFKKTFCEGMGMKIMRYRANSIHASLEIRSNGDGET
                     AVICTF"
     gene            22240..22431
                     /locus_tag="PK125_02430"
     CDS             22240..22431
                     /locus_tag="PK125_02430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB36999.1"
                     /translation="MITFEMTKDEANIVQNVIERYLYHLQVEIMHTDKREFRDALKQR
                     EKFLKDIIDRMKTKILAEP"
     gene            complement(22503..24212)
                     /locus_tag="PK125_02435"
     CDS             complement(22503..24212)
                     /locus_tag="PK125_02435"
                     /EC_number="6.3.5.1"
                     /inference="COORDINATES: protein motif:HMM:NF010588.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD+ synthase"
                     /protein_id="HPB37000.1"
                     /translation="MTENWRIAIAQINCTVGDFRGNSAKIIEYARKARGSGADIVVFP
                     ELAITGCPPEDLLLKPKFIEDNIRAVEEVARSIKDIVAVVGFVDRDNGKIYNSASVIY
                     DHEIKGVCRKTLLSNYGVFDEKRYFSSGDESLIFKLGELIFGVNICEYTWFDEGPTAS
                     QAASGAALILNINTSPYQAGKIKVREEIVSHHASINRVFIFYVNLVGGQDELVFDGQS
                     MVCDNTGAIIARAAAFSEDLLVIDIPRNELIKEQTGKLETDGATKLEMITLSENVEYA
                     DKPAITWHQREMLNPVAEVYKALELGLHDYVAKNGFHKVTLGLSGGIDSAIVATLAAD
                     ALGKDHVTGVFMPSRYTSKESEEDARELAANLGIELLTVPIDDVYMAYISTLLPLFKN
                     RKEDITEENIQARIRGNILMALSNKFGWLVLAAGNKSEMSVGYSTLYGDMAGGFAVIK
                     DVPKTLVYTLSAYRNSLGPVIPQRILDKEPTAELRPGQKDRDTLPPYETLDSILKAYV
                     EEDKHPEEITSSFLAKDVVIQVSRMVDGSEFKRRQSPPGIKITPKALGKDRRMPITNR
                     YRG"
     gene            complement(24307..25530)
                     /locus_tag="PK125_02440"
     CDS             complement(24307..25530)
                     /locus_tag="PK125_02440"
                     /inference="COORDINATES: protein motif:HMM:NF024883.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF and ANTAR domain-containing protein"
                     /protein_id="HPB37001.1"
                     /translation="MKLSEKIEQKEQELKILHEVAKDISSNLELKELLNRIISMIMNF
                     ITADSCLIYLYDRQDDELILTASSKPKEKSIGRVKLKIGEGVTGWAAKEKRPVVLPKE
                     AYKDQRFKTFSSLEEEKYEAFMSIPILSKDEVIGVMNVRNMEVHRYPEYQVNLLFTVS
                     RYLGSAIHNAIIYEDVIRKAKQLDLLSEVSRTIVSNHYLKEILQLIVTMTAKVMDSQI
                     CSIMLLDEKRSELFIAATQSLSRGYLNKPNLKVGQSVSGRVVLEKQPVTVSDVRQESG
                     YMYPEVARKEGIVSLLSVPMMVKDRAIGVINCYTKKEHQFKPEEISILQTVANQAAVA
                     IENTHLSEEILAAREALESRKIVERAKGILMKDLDVSEDEAYKKIHKKSMDLRKTMKE
                     IAEAIILSSELRKKS"
     gene            26026..27741
                     /locus_tag="PK125_02445"
     CDS             26026..27741
                     /locus_tag="PK125_02445"
                     /inference="COORDINATES: protein motif:HMM:NF014763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPB37002.1"
                     /translation="MQQKMSLEKLRVNLIIILILISSVPLVLFAIVVYRKEADLVRKK
                     VLSHLKSIVAQNSLMVDEFRLERMRDLSLLAATIEAHGGECDRIHLKLMHKDRTIYKH
                     IFFADKEGRIRCKTGKGHLFERGLVNQAWFKAAAKGKPFMGELTSREGKDRYDLLLSV
                     PVRMANGTTIGVAGAIVDFDNIASIMKNTGIGQTGEIYILNKDGVFLTSTQVEEGRSG
                     ERMEPELFAGYFKEVGPNEYIDYRGKKVVRVFKKLSDSNWYLVGEQDATEVFKDVANL
                     RNLFAGFVVLLIALITLIGYLISRKIVHLLKSAYEQKKELEIQVIQKDKLASMGLLTA
                     GIAHELNTPLASALLYTQMLKEDARNTWPVALEKLSSIEGEIKRGSKIVKNLLDFSRQ
                     SQTDTVTTDVNEVLGKLLDISEQLCSDRGIEIKRTFEKKLPLVKGNANILHQVFMNIV
                     ANAVDAMEKGGTLSVLTRHIASLHKTVIEIQDTGTGIPEELLGDVFDPFFTTKPSGEG
                     TGLGLAISYSMVRKMGGNIRATSTCATENSYPYQPTGTTFTIELPALDDDTQSTVKER
                     MVKTQ"
     gene            27738..29093
                     /locus_tag="PK125_02450"
     CDS             27738..29093
                     /locus_tag="PK125_02450"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HPB37003.1"
                     /translation="MKPSQILIVEDDRKMRLALREIMTKEGYSVDTVETGEAALGRVE
                     ETSYDLVITDLKLPGIDGMSLLKAIRKSRPDTSVVIITAYATVDTAVEAMREGAEDYI
                     SKPFNLDEIRIIVKKVLEKKALLDDNRLLRNQLKKKYSYDNVVGNSEAMIEVYKTIDK
                     VKDAKATVLILGETGTGKELVARAIHYNSTRSGEPFLPVNCAALTESLLESELFGYVK
                     GAFTGALKDKLGVFETADGGTVFLDEIGDISPRLQQVLLRVLENGEIQPVGSTARKKV
                     DVRVVAATNKNLEELMKTSLFRADLYYRLNVISLRLPSLLERKEDIPALANHFLRKYS
                     AENNKTITGISKEALQMLEGYNWPGNVRELENVIERATLLETTDEITVDSLSEKIRSS
                     LNRSPVVLEEDLRTLEEVGRAYIIEVLEKTGGNKVKTSEILGINRTSLWRMMQRLKIV
                     A"
     gene            29262..29693
                     /locus_tag="PK125_02455"
     CDS             29262..29693
                     /locus_tag="PK125_02455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB37004.1"
                     /translation="MNIRFIDKHRDFQITYDRLLELKNSFVLDQHQQFLCRALRQDGN
                     WRLKERILEFAIDIRKPTDDFIENICAVMTNRNTSINARILAADALRVVVPRKVRQTM
                     AYPTFRGASVLRVMKDILKAPEVPELHEAVTRALKAIQEGS"
     gene            29721..30758
                     /locus_tag="PK125_02460"
     CDS             29721..30758
                     /locus_tag="PK125_02460"
                     /inference="COORDINATES: protein motif:HMM:NF019284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB37005.1"
                     /translation="MTHRDYTPEVTRVPTGYNCDGMKIITYFSDGKEIAKEILCDNGL
                     QLVMTGTIPDGTVLEYYWVGTLHRVFTYANNRAHGRSHTFYPDGAIWMEQEFIDGLLH
                     GPMKTFYKGGTIQEECTYKSGRLHGELKRYYEDGTLDTLAYFNEGKLDGDYCTYFQNG
                     MPREKSIFRDGIREGNSIKYYETGELQCIDLCLEGRVAHRKRFDERGRLISDQSEPVA
                     EIEEEKSIEAKEHMNRGMDLATMGCHKQAAEEFQRAISADPFTYEAYLRLAVAYRRLG
                     FYGDCIDTLGKLLEINPHHLEARFNLAIAHVVTGNRGEALAGYHVLRDIDEGYAHGLM
                     TILESPRLHLQ"
     gene            30967..31272
                     /locus_tag="PK125_02465"
     CDS             30967..31272
                     /locus_tag="PK125_02465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB37006.1"
                     /translation="MENEKDRCEQDSFSGFIFNKPQRFKESLRGWVNHFHNIGCKFVV
                     LQQGVTGQYVIFKGSQDTREDMDRSVPLSKVLNNGYRMVEYLDMTDEVRNHKHDTMA"
     gene            31406..31909
                     /locus_tag="PK125_02470"
     CDS             31406..31909
                     /locus_tag="PK125_02470"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HPB37007.1"
                     /translation="METTRDFFQGTEAAPILTRNDNDTTLTNPEHIFSFLSLTQNRAG
                     DQPMSIAPFKFRSACKRAPVGMSVVKIDDGKVLLSNPAMQNLLGYSRQELIGMPFSQF
                     THPEDVEKQKEAYLKIKSGKRVSFDLKKRYIRKNGTSFTAHLVSTLIRRSDSKPQVMI
                     EMIMEEA"
     gene            31936..32997
                     /locus_tag="PK125_02475"
     CDS             31936..32997
                     /locus_tag="PK125_02475"
                     /inference="COORDINATES: protein motif:HMM:NF014445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016755 - aminoacyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0044038 - cell wall macromolecule
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan bridge formation glycyltransferase
                     FemA/FemB family protein"
                     /protein_id="HPB37008.1"
                     /translation="MQLDLRPKEVYGLFPTDILFQTAYWSQVKSRLGWKALAFDFSTS
                     GHSGDVLVLAKNFGNKFSAAYVPQGPEYGPEPEDYGRFLETMSCSLVKYLDPKPTFIR
                     YDLPWESHYARDIAQGEYSAPPETRLVELRMNFGTQTWNLRKAVTNMTFADAYVVDIT
                     PSEEEILHSMKPKTRYNIRLAKKRGVHVFPASVEMLPKFYDLYRQTAMRDGFYLCGYN
                     SFLALFSPMPEEANSPEVHFLIAAHDNDLLACAIITISGKTATYLFGASSSEKRNLMG
                     PYAVQWAAICLARSRGCHVYDMGAISPTKDPAHPFFGLYRFKSGFGGRIVHRTGSWDY
                     PLDQNLYNTFRNSEMMSRE"
BASE COUNT         8642 a         8107 c         6912 g         9296 t
ORIGIN      
        1 acacggtcag cgagaccatc ttacagatgt tttaatgtgt gatttgagtt taatctttct
       61 taggttcatt tcaccactct taagaggcgt ttcttacctg tttgcaggct gtgtaacgtg
      121 cattcgcttt aatcaaatct caaatttacg aggtggtttg tcgctctcac tccaaatttt
      181 cccagggttc atgatattgt tggggtccag gagagtttta atttttttca ttattgtgag
      241 tacagttgga tctatagttt tcattaaaaa cttttgtttt tcaagcccta taccgtgttc
      301 acccgatata actcctccaa attcgacggc tgcttgaaaa atttcatcca tggcttctgt
      361 agccttctgg tagtggttca catcggtgat atcagtcagg attgccgggt gaaggttccc
      421 gtcgcctgca tgaccgagtg cgactatttc aagattgtac ttcttggcca gttccttaca
      481 cctttttatc agtaccggca gattacctct gggaactgtg acatcctcaa cgaggacagt
      541 ctttgtatct ctgtaaatag ccgaagcacc aacacgcctc gccatccaga attgattggc
      601 ttcattgata ctctcggcta tcctcacttc ttttgcacca tgttttcgtg ctacctccac
      661 aataatctcg gtctcccttt ccacagcctc gcttgcacca tcggtctcaa aaagtagcac
      721 tgcatcggca tctctcggga gcccgaacgg catcgtttcc tctattttgt taattaccca
      781 gttgtcaatg tattctatct ttgttggaat tacacctgct tccagtaccc tggaaacatt
      841 ctcgcccgca atgactgcat caccatacac ggccatgact gtctttcttg caggtggcaa
      901 cagcatcagt cttaaatctg ctctcgttac cactccgagt gttccctcag aactaatgat
      961 aatgtggaga agatcgtaac caatgacgtt gttcaaagtc ctcccaccaa gatttattat
     1021 ttcaccagta ggaagaacca cttccattcc caaaacgtat tgttttgtca ctccatattt
     1081 caggcaagag gggccaccag cgttctcagc aataataccg cctatcgtgg caccataata
     1141 actctgggga tcaggcggga aaaaaagccc ttctttggct aactccagaa ctaaatcctg
     1201 aagcacaacg cccgcttcca ccgtcgccat catgttctct ttgtcaattt ttgttatcct
     1261 gtccatcttg gagagacaga ggactatgcc acccgaaatg ggtatcgaac caccacttac
     1321 atttgtgcca ccacctctcg gtgttacagc tatcttttct ctgttcgata atttcatgac
     1381 ctgtgagagg gcttttgtat tcaaaggaaa tataactaca tcaggtttgt gtgtccacgt
     1441 cgttgttcca tcgtaagaat atgttttcaa acattctggt gatgtaaaaa cattttcttt
     1501 tcccactatt ctttgaaggt ctactattat cgactctttt atcataaaca ataccctatc
     1561 atcatcgtgt taattctttt actgaatcat gctattcccg gaatgacgct gcctcctgat
     1621 ggaaaaattg ccacatcagc cttgggcata ctcttgaacg tagcagagac cgcatcatca
     1681 agatttgaag cgtagatgaa attcatcatg tctacctgct cctttgtcaa tgcctccgtc
     1741 acatggacaa cactgagttt ctctgataat tccttaaaaa taacaaccat catgataaat
     1801 gaaataccga aatttttgag atggtcagga atatctcctc gtatcaatcg atggtgatat
     1861 tcatttgccg tttctttggt agccatttgc tctacaagtg gcatgatcgg acctgaattt
     1921 ttttgcgatg cgacccaaat gatggtgcct cctgtcctga cggcaaaact ggcgttcaac
     1981 agtgatttca tggcattaac gcctatttca agcggagctg cggatgttat caccacatca
     2041 ggtcgtttgg ccaacggaat aagatagaga gatgaggcaa attctgatgc cttcttgtgt
     2101 gcttcaaccg ggtcacccgc aaatgccttg attacaccat gtctttcgtt cataacgaaa
     2161 tcaagcttaa aactcagtct gcttaaacgc cctgcatcta caatgtcctc ataaaaggga
     2221 ttcccctcca gtaaattaag cttgcagtta cggtgtcgca tccacgtgta atgatggtct
     2281 gccaccgccc gataagatgc tactcctggc atgacaatct tgcatccacc tccaaatcct
     2341 gcatatgggt gaggcatgca ctcccctatg ccaataatca aatcactttg ggcaacaagc
     2401 ttgtttacct caaccagcgt acctttactg gttcgaccaa tcagaatatt ctcatttgaa
     2461 cgggggtcat ggttgtagac tctaccctca agacggcgaa atgtttcctt gccagccttt
     2521 ttttcaagtt gttccctggt atgcaatggg tgtgtcccta atgcgcaaac ggcacttatt
     2581 cgttcatcgg ggactcctgc cctgttgagc ctgttcaata tgtgcgggaa ggcgagatgg
     2641 gacggtgtcg gtctctgaat gtcgtcgaaa agcaccacca cgtccatccc tggcttacca
     2701 agctcttcaa ggcgcaaaga cccgatcgga ctatcaagga cacgctcaat ctctctatca
     2761 acatcctgta cttcggatac tgccgaacaa tcctgacagg taagtacatt ccagcctgat
     2821 ggcagggaaa agtttattct accaccttcg taattcaaat agtagtccat ataatttcct
     2881 cctattcggt cacgattccc tgattgtcag atcaacattg acaatcttta accttatttc
     2941 tgagattctc ccactccact tcaatttgtg tctgcatgtt tgctatctgg tcctgggtca
     3001 ggtggccata tctaccctgc atctggagat actcatcaat ggggaagcct tgaggttcat
     3061 atgttattac atatcttctg ccgtcaaaca cttcatacag agggaaaagt cgtgtctcta
     3121 ctgataacat ggcagccttt accgtgagac cctcagccat cctccagccg gtggcgcagg
     3181 gagtaagaat atggaggaat ttggtccctt gtatgtttct tgcttttttt accttatcca
     3241 taagatcttg aggataccct atcgaggctg ttgcagcata gggtatccgg tgcgcagcca
     3301 cgatctccat gatattcttc tttctgaaag gtctccctga tggtgtggta atagtccaag
     3361 cgcattcagg ggttgcagaa ctcgcctgaa tgccggtatt catgtatgcc tcattgtcga
     3421 gacacacgta gatataatgt tcattccggt ccacagtgcc tgagagtgcc tgaagcccta
     3481 tgtcaaaggt gccaccatct ccggcaaaag caaccactga tatgtcatct ttcccctgca
     3541 gcttgagcac atatgatatt ccagccgctg ttggtgctgc tgattcaaag gtgccgtgga
     3601 acgcagggac cttgagggag gtattcggat aggtggaggc gatgatggaa gagcacgacg
     3661 gaatgatgac tgctaccgtt ttttttccga gggcgcgaag cacaagcctc atggcaataa
     3721 caaccccgca tccaggacag gccatatgcc ctgatctcat catatctact atgacctttt
     3781 cattttccat cttagctgcc tccaatccat acaggaagat cattcggttc atctttgccc
     3841 atgacttcca tggcaatacc ctcaagagta tccggagaaa catcgaggcc atcgagtccg
     3901 gctatgaaac cgtatacagg aggtcggata tctgaattgt agagggaaga cttcaattcc
     3961 tggcaaaaaa taccttcatt tccgagggag atgttacgat ccaccaccgc cacttttgca
     4021 acaccttgca aggcgctttt aatctcccct gaaggaaatg gcctgaaggt ctttatcttt
     4081 agaagcccaa tctttaatcc tttgttcctt aaagaatcaa ctgccacacg ggcggtactt
     4141 gtcattgatc cgcttgttac aagaaccagt tgtgcgtcct ctgtttggac cgcctctacc
     4201 attccgtatc tcctgccaaa atgctcctcg aactctttgt ccactttttt gattacagtt
     4261 tttgcattct ccatgtcgtc aaagcccctt ttccgaaagg gcataaactg ggaaccagta
     4321 gtcaccacat tcgagacaaa agtagggttc tctatatcga atcgttcagg aagggcagcc
     4381 ttaggcagga accggtcaac cgcgacctga tccggtactt ccatgggctc gatgaaatgg
     4441 gaactgaaaa aggcatcaat tataaccatg gagggaagca tgactatttc ggaaattttg
     4501 taggcctgga taactgtgtc aaatgcctcc tggttatcct cacaatagaa ttgcatccat
     4561 cctgtgtccc tctgggcaag tgtatcggtc tgatcactcc caaaagacca tgggacggca
     4621 aggactctgt tcacacaccc catgacaacg ggcaccctgt tgcctgacgc ataatggagc
     4681 atttcgtgca tgagcgccaa tccctgtgag gaggtggctg taaaaacccg cgcgccggtg
     4741 agagacgcag atatgcacat ggccattgcc gagtgctcag actctacccg taccatgatt
     4801 cccggcagtg tcccctgagc ctccatttct gagagttttt cataaattgg agtttgaggt
     4861 gttataggat atgctgggat aagctgggcc ttggccaacc tcaccgcttc cgcaattgca
     4921 tagtttcctg ttaagagttc cctcattggc cgtcctccat ttctatcacg tggcgggggc
     4981 atgttattgc acagatactg catcccttgc aatagtcttt atttacgctg tataaccgct
     5041 gtccttcctt gataatggat atgtcnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5161 nnnnngcctt ttccgagttt gagggatgtc attaccctgt tcggctcata tccgtttacc
     5221 tcgagatgaa tggaaagagc agcccgtttt cccgaaagaa tggcatccgc cactgcaggt
     5281 cgctgccgga tcacgtcccc gccggcgtaa aaaccggaca gcggagtgct gcccaattca
     5341 tcgacaaaga ctctggaaga ctccttatcc actatagatt caaaaaacac gcttgtatca
     5401 gggatttggc cgattgcaat gatcgcaagg tcagcatcaa ggaatcgatc ttttccctta
     5461 accggtgaca gtctgtatct ccctgttgcc tggtcttttt ctacttttgt tttgatgaac
     5521 ttaatctgtt ttaggtgctc tttgcctata aactctatcg gctggtattc tcctatcata
     5581 tggattcctt cttgctctgc ttccacaagc ccttcctgaa tagcagggaa ttcgccttcc
     5641 ttctcgggag cgaccatcgt gattccggca tcggggtcac agaggcggcg tgccgttctg
     5701 gccacgtcaa cagccacatc tccccctccg acaacaacaa cattcttctt tccttcacac
     5761 atccctgttt tgtctccatg tgaaagaaaa tcaagaccgt atattatggt atcccggtgt
     5821 tcgaagctcg cctcaattct ttgtggcacc catgcccctg ttccgagaaa gacatagtca
     5881 tatttcctct ttatatcggt cagtttttcg gtgtccacgc gctcccccgc aaccagatct
     5941 atacccaacc caagaatccg gtcaatttct cttttcacga tttccctggg gagccggtac
     6001 tcagggatac catatctgag gacgccccct ggttcaggcc ttgcctcaaa gatggtaact
     6061 ttgtgtccaa acagtgcaag aaaatacgcg gctgacagcc ctgcgggccc tgaccctatt
     6121 actgccacct taacctggtt ccccttttgg ttggtccaca cttgaggagt tgcttgcccg
     6181 tagtccgcaa tgaagcgttc cagacttcta atctcaaccg ccttatcaaa ttgaatcctg
     6241 ttgcagttga cctggcaggg atgatagcat acgcggccac atataccggg gagagggttc
     6301 tcgtgaagga tcagggcaag ggctccgtcg aagtcaccct ttgccacaca ggaaagaatt
     6361 cccgggatat cattgcccac agggcaggct tcgcggcatg gagaaagctt ttctctgtac
     6421 aacggttttc gcggactcca ctgacctgtt ttgaaaatct cggtactgag cctcgacaat
     6481 gggataatgg tttcatggtc aaccaactta atattttctt ttctgatcat taattattac
     6541 cctccaccat aactgacgtc atctcatacg agtgtaatgc tgccttcaca ttcttttcaa
     6601 tctttgcagg aacagaagtc cggatagcat tttcgagtat aagaacagac cataaggatg
     6661 tagtattggg tatcttccca tgataaatat gcttttacca gtatccgaac agtaatagcc
     6721 accccatgcc tccgggccaa aaaatttaat ctcaccagta attataaacc taggtagaat
     6781 gcatcatatt ttcagattgt taaatggcac ctaataattt tgatatcaat atcttttcat
     6841 ttcattaaat atccttgcaa ccactaaaca gaacttaaaa ataacaatga attattggta
     6901 tacggtatac caattttaaa aataatgtca agttttttat aggcttgcaa tgattttctt
     6961 attgtgtgat gttaagctta taattacaga tagttacgat attttgattg gcagcttttt
     7021 tacgatacag tgctttattt ggaaaatagt ataccattgg gcagggatta tgtcagatac
     7081 ggtccgtttc tgacagttct tctttgggag cagccattaa aatgtctctc aaagaaagtt
     7141 caaggtggta atggattgca cgtgctacct catcacaatc acgcctttct atggccttat
     7201 atatgagcct gtgtccatcc agagctctca gaaaaacgcc tgagtgatag aaacttgcaa
     7261 tcctgtgccg aaccatgtgg cgtctcatca actgtgttat ttccataatg taggtgctgc
     7321 cactgaggcg cgctattgtt tcatggaatc gagcatccag gtccggatag gaatgaacat
     7381 cttgtttgga tatcttatct tcagattctg aaatgatttt tcctaattcc tgtattagaa
     7441 cgggataggc cttttccata gcccactcag cagcaagcgt ctccagggct tccctgattt
     7501 tgcatacttg aattacttct tctttgctga tcgatttcac tttgtaacca acccgaggta
     7561 ttgcctcaac gaacccatct ttctgaagat tatggaatgc ttccctcacc ggagtccgcg
     7621 aggtcccgat ttctttggct actttagctt ccacaaggcg ttgatttggg tgtatttcac
     7681 cggatattat cttttctttg acatattcgt aaaccttaac ccatattgca gtatcattcg
     7741 taaaatcaag agtcatcata atttaggata ctgtatacca gtagatttca gaaatcaagt
     7801 atttataggc gtgcaacatg gtaaatgtct ctgttttgca ttactgctta ccaacgaaac
     7861 tttttttcta tacatcatcg ttgttaatta ttgcaaatag cctctatttt tgtttaggag
     7921 ctcttttgaa ctaacttgac gtcgacatgg tcgattgatc gcctggtttc cgggaaaatg
     7981 gaaagaatgg tttatgaggt atgttccaag tcgcataaca catagaaaaa taattatggc
     8041 ctacatctgt tgagtttgca aagattgtca aaaaaatgat ggatgatcag gagatagaaa
     8101 agattgaaag ctgctacatt gactatttgc agatgaaaca ccttccttta agaaatccaa
     8161 acttcagtgc gttgaaggtg gcggattaga agggcttgtg tcaaggtgct atgggacgtt
     8221 tttcttgtaa tgccgaaggg gaagtgagat gaggaacaga actcaaacag aataagacac
     8281 cagtgccata ttgaattatg gattaaaaag atatggcatc agggtcgtgg atggagacca
     8341 gcagccttta cgatctcctg caccttctct tcccattcaa tagccataat gtggacacct
     8401 gcgacgccct taattgcccg cacacgttcg attgtctcaa cggctatttt gataccctct
     8461 tcagtaacct tttccttggg aacaccttta aggcgttcga tctgctcctc aggaacgtcc
     8521 ataccaggaa cagagttttt catatattga gccattccga tggatttcat gggtgtaatg
     8581 cctgcaagga tatgaatctt ctcgtgaata ccaagatcac gtaccatttc catccatttc
     8641 tcgaattttt ccacattgaa gacgcactgg gtctggacga actgggcacc agcagcagcc
     8701 ttttttgcaa gccttaaagc ccgaatctca aagggatcag caaaggggtt ttccacacaa
     8761 cctatgaaaa tatttggttt gcctttaact tcttggccgc tgataaacct gccctcatcc
     8821 cgcatccctt tgacagtctg gatgagctga atagagtcta tgtcaaatac attttttgcc
     8881 gtggggtgat ctccaaattt ctggtgatcc cctgaaagac agagaaggtt gttgatgccc
     8941 aacgcagcag ctccgaggat atcgctctgc attgcaatcc tgttgcggtc tctgcatacc
     9001 atttgcatta tcgggtcaag acccacccct ttgagcacca ggcacgcaga aaagctactc
     9061 atgcgaacta cgcttgtctg gttgtccgta acgttaacgc agtctacata tccgagcaga
     9121 taactgccct ttttcttcac aacctcagga tcagcccccc gtggagggcc gcactccgat
     9181 gtaacagcaa aggtcccgct tttcaggacc cgttcgaggt tgctctgcgt gctcatattt
     9241 tcaagtcctc cctcaccctt ttccttggac caccgtccct gctcgttgac caatctttca
     9301 tgggctggat ttcaaggtat ttatccatca tattcaactc tctgtaacgg tccactataa
     9361 ggtgccaggc acacggaata tccctgtcca cctcacactt gccgccctgg gagcctccac
     9421 atggaccgtt aaatatactc tttgcgcatc gagtgacagg gcagattcca agggttttgt
     9481 ggagaatgca gttaccacag ccctgacacc tctcagtcca tacaccatgc tcttcagtga
     9541 caccataaaa ggtcgtgttg actgccggca aaaccagcct atctctgtaa tgctctccta
     9601 caaattgaat cccacagcca cacgccatag ataggaccac ctcgtaatca tgaatgaaag
     9661 gtttcagact ctcaacatac tcgggatcgc agttgcgctc aagcgttacc tcccctatct
     9721 caacaggttt tccttccttc tgcctgtaaa ggcgaatctc agatgccaga accgcaacct
     9781 cttttgaacc gcccgtggca cagacagtta cacattcgtt acagccaagg aggagtatct
     9841 tcgaataggg cttgagcatc tcgataatct cttcaaaggg ttttctgttt gctactatca
     9901 tttttggctc cttgttatct cagctctcag ttattgtcaa atacatatta ttagcctttt
     9961 gctcattttt gactgctaac tgccaaacgt ttacttttcc ttttttgcag gatttggccc
    10021 tagtgttcgg attttctctg tcatctctct tgccacttcg gcaaaacgtt gcccctcagc
    10081 tgaagacatg ttatacattg ccacgcgctc accccccacc ccgcattcat caaggatatg
    10141 tttcaaatac tctacccgtt tttttgccct aatatttccc acaaggtagt gacagtcacc
    10201 ttcaaggcac cccacaagat aaacaccgtc tgcaccgtcc tcaaaggctt taagaacatg
    10261 agtgatatct accctacctg tacaggggac tttaatgatt ttcacatttg taggataaga
    10321 aagacgcatc gaacctgcca ggtccgccgc agtataccct caatattcgc aacaataggc
    10381 aataatttgc ggttcaaatt tccccattag aattcctcaa ctacagttca tgtttcgaaa
    10441 gcaaatgtcc tcggtctcac cgtcaacctt ttcttcatcc tgttctttta aatctgcaat
    10501 cttcattttc acgattcaca ttctgctacc atgtcgcctg ttacttcaga aaaaagggat
    10561 ttagctttgc ctataatctg agcatctctg taatgcatca ggtcaatggc cccggcaggg
    10621 cattcagcaa cacatatgcc acatccctta cacttactga tatcgatctc tgcatcacct
    10681 ttttcattga tcaccgggac agagtatgga cataccctca cacatgtcag gcaggcagca
    10741 cacttttctg gctcaaccct tgcgactact ccgccggctg cgatttgatc ttttgacagg
    10801 atcgttattc ctcttgcaac ggccgcctct gcctgggcaa tactttcacg aatattcttc
    10861 ggcgagtggc atagtccagc caggaacatg ccgtctgagg gaaaatcaac aggcctgagt
    10921 ttcatatgtg cctcaagaaa aaagccgtct attgttctcg gaaccttgaa gagggttgca
    10981 atggcttcgt tgtctctcgg cacaatggca caactgagga caagaagatc tgttgtaaag
    11041 ctgacctctt ccgtgacaga agggtcaaaa catgtgatag caagcacacc gttaccattg
    11101 ttaacgattg gaggattcgc cttctcaaac ctcacaaatc ggatgccgag cctccgcgcc
    11161 tttaaatagt atttctccag aaaaccatag gtcataatat ccctgaaaag gataaccaca
    11221 tctgcttcag gtttttgttc tttgagggtg atggcattct taatggccgt cgagcaacag
    11281 attctgctgc aatacggacg tgcttcgttt ctcgatccca cacactggat catagttacc
    11341 ctttcgtagc gtggaagccg gcctgatatg atctggctct ccagctccgt ctgggtcagt
    11401 actttctcgt tctctccgta gagaaacatg ccgtttggct tgtattcttc tccacctgtg
    11461 gccaaaacga tgataccgtg gttgatcttt ttgtatttct tgagggggcc cgcctctatc
    11521 cctgtctcaa aatttccccg gaaacctgtg tgatcgatta tctgagcctc tgtttccaca
    11581 tgtatcaatg gatggctgtc aaccctttca attgtctcct tcatgaatga tctgacatcc
    11641 agaccgtcga tggtagaaaa gatatgtttc aggttgccac cgagagaatc ttcctgttct
    11701 atgagaaaga cctcaaagtt ctgatttgcc aatttcaggg ctgctgtcat accggccatg
    11761 cctccgccca caacaagggc ttttttattt acatcgagca tgatgtcctt aagaggcttg
    11821 atgttgttgg cgttcatgac agccatcctg acaagggcct ttgccttctc cgttgcaccg
    11881 gccttctcgt gcatgtggac ccacgaacat tggtctctga tatttgccat ctcaaaaaga
    11941 tacttattca aaccagcgtc tcggatcgta gccctgaaaa gcggctcgtg agtgcgggga
    12001 gagcaggacg ctaccaccac cctgtttagt ttataatcct cgataagctg cttcattttg
    12061 tcctgggtgt cctgagaaca cgtgaacagg ttctcctcag aaaagaccac attgggcaaa
    12121 cctttcgcat aggattgtac ttcagggacg ttcacaacac caccgatgtt gatcccacag
    12181 ttgcagacaa acacacctat ccggggatcc tcagcatcca catctttttc cgggggcagt
    12241 tcctttatga caaggtcttt ccctcttacc tcagagataa cggacccagc ctcgcaggca
    12301 gcaccgcttg cttgaattac tgtttcaggg atatccttcg gagcctcaca cgcgccactt
    12361 acatagatcc cttccctgga ggtaataagg ggattctttt ggctcgtttt gacaaatcca
    12421 tactcgttga gttgaatacc caggacattt gcaagtgtgg gaagatgctc tgaaggtctg
    12481 gtacccaccg ataaaacaat caggtcaaat tgctcggacc tgagattccc gctctcgtca
    12541 acgtaaacta tcttaacatc cttggttacc gggtcttcca ggattcgtga tacaaagctc
    12601 ttgacgtatc gcacgccgta ttcagacact gcacgctcat agtattgatc aaatcccttc
    12661 ccaaaaaccc tgatatcaag gtagaaaatt gtggtctcca tatcattctg atgttctttt
    12721 gcaatgatgg cttccttggt cgcatacatg cagcatacag aggaacagta ttcgtgcact
    12781 ttgtccctgc ttccaacaca ttgaacaaaa gcaatcttct ttggtatctt cccatcagac
    12841 ggcctcatta caacacttgc cgtcgggcct gtggcagaga gaatcctttc gaattcaagg
    12901 cttgtgatga catttggata cacaccatat ccgaactcct tcttttgtgt tgcatcatag
    12961 gggtcaaaac cactcgtcac gatgatgctg ccaacctgta tcctctcttc tttcggagtt
    13021 tcgaaataat ctattgcttc gggcttgcag aattcttcac agatcctaca tccaaggcac
    13081 cttctgcaat tcaggcaccg ttctgcttcc ctctttgctt cctcctctgt aagggtctga
    13141 tagacttcct gaaaacctgt cctttctgca accggcaagg attggattgc tactctggct
    13201 tttttctcca taaaggtggg aacatcctct acaagtttgg tctcgatcgc ccttctgccc
    13261 tctctcagat tccgtccttc gaggtatctg atgattgact ccgcagcatc tttaccctgg
    13321 gcaatagcgt cgatggccat ctttggaccg gtaaccacgt caccaccgga aaaaatccct
    13381 ttaacagtcg tctcaccagt gaccgggtcg gccttgatac ggccgtcgtg aaaaacttca
    13441 agatcatcca gctctgacag aaaagccgtg tccattgcct gtccgatggc gatgatggct
    13501 gtatcggctt catggtaaat tgtagtctct tcatcgtact gggggttgaa cctaccatac
    13561 tcatcaaaga cagacgtgca ccttttgaat tcgataccct ttatatgacc atgggagaca
    13621 tcaaagcgga gtggtcccca cgatggatgg aagattattc cctcttcaac ggcctgacct
    13681 atttcccatt tgtgagcagg tatttcatcc cacttttcaa gacagactac atgaacttcg
    13741 cgtgctccaa gccttctgct ggtaagggca gcatctattg ccacgttacc ccctccaatt
    13801 atgaagactc gctttcccac ttcaacgggt gagccaataa taacagattt caggaaatcc
    13861 aaaccactta atacgccctg agcatcctca ttcgccagac caaggcttct cccaaggtgc
    13921 gctccaactg caatgaaagc tgcatcgaat gaattctgga tatcctttat tgagatatcg
    13981 cgacctacct ttgtattcaa ccgtatctcg atacccattt tttcaacaat ggcaacctct
    14041 ctcgccaatt cgtccttggg cagccggtag ggtggtatgc ccacgtacag catacctcca
    14101 agcttgtcgt aggtctcaaa aatggttacc ttgtatcctg cttttcgcac ctctattgcg
    14161 catgtaagcc cagcaggtcc tccccctaca attgcaacgc tctttccttt atcaggccct
    14221 accccaggaa tcggaatctc ccgtttcccg acttcataat ctgcaacaaa tcgtttgagt
    14281 gcacaaatgg caatcggttg gtctaccttc ctgccgcgca gacattcttc ttcgcaggga
    14341 tgggcacaga tacgtccaat gattgatggg aaaggaagcc tctcccgtat gatatctaaa
    14401 gcttcaagaa accttccttc agcaatgagg ccaacgtagt ccctggcatt aaggttaatc
    14461 gggcatcgat ctacgcaggg cgctatctct ctgtcaatca tgtaagtcga tgggatggcc
    14521 tggggaaaat tgatgaaggc tgcgcttcgc tgagacatac ccacgttgta atcgctaccc
    14581 acctctaagg gacaggcagg ggcacacaga gcacaggatg tacatttttc aggcaaaacc
    14641 ctccgtggct ttcttcgaag cgtcactgta aagttgccag gttctccggt cagatctatc
    14701 acatcagtgt tcatcatgag ttctatgttg gggttcctac caacttctac gagctttgga
    14761 gccatgatgc acatggaaca gtcattggta ggaaaggtct tatcaagctg tgccatacgc
    14821 ccaccaatgt ttggtttttc atcgataaga tacactctga aacccgcctc gacgagattc
    14881 aaagaggctt ccattcctcc aattcctcca ccgattacaa gtaccttacc taccttgtct
    14941 accttttcca tggtctgtcc cgtacgtgaa acttctttgc tgcatttata ccttaccgcc
    15001 ttaccttttt aaaaacagct tctcttcccg catatccact gatctctacc atattctttc
    15061 ccaggagatc cttcatgtga tcaaaaacct gctgcagtct cattcctgtt gccactgaca
    15121 ggtcatcaat tgtactgtca ttttttgcaa gtgtctccag gatacgcgcc ttctcgaatg
    15181 ctgcctcgag gagagaggtg aatacaagag aaaactgcct gccggtcatt ttttcaccgt
    15241 acacattccc ggacatcaag gcgtcttttt tattgagaag tcttgtgagt ttctcagccc
    15301 ctccggggta gtgtgttttt acgtagtcaa gtttctcgtc aatgtccatg accaataccg
    15361 gatttatcca cttatcttct tcaccttctt tacagcctgg tccacggcta tcacatgaga
    15421 gttgctgccg cttagtcttc cagagatggc aagctcaaaa acctcggata gaaagagaat
    15481 gggaagagcc tggtcagtcc tgccaagata gggtaataga tcgaggttca actggcagag
    15541 cggacatggc gttacaatta catcagctcc gagcaatact gccatatcca tgatcttatc
    15601 tgaaaggtca taggtaatat ccttatccgt cagcgttttg ctcgcaccac aacactccat
    15661 cttatatggc cagtttacaa gctcaacccc tagagcccac agcaatgaat ccatgctcgt
    15721 tggattttct ttatcgtcaa aggcctccat acccatgacc ctcgtgagaa gacagccata
    15781 ataggggaca accctaaggg gagagatgtc atatttaagc cttccggcaa taacctcaat
    15841 ccccacacgg tcatggaaca cttcaatgat attcttaacc tctccagtac ccaggcattt
    15901 gagtggtgaa ataacgctgt taatatcctc cctgaaattt ttgtcctgtc tcatgcgctc
    15961 atttgtaacc ttcgtccgta tgtaacatgc tgaacagggg gcgaacagat gattgttctg
    16021 tttttctgca agggcaaggt ttcttgcagc cagagcatgg gcaagtctct catctaccgt
    16081 atttgctgca gatgccccgc agcatgacca gtcgggaagt tcctgaatct ctatcccgta
    16141 aaaggaaaaa acttgcttta tggatgtttc atagaacttg ctcgatgtct taagtgaaca
    16201 gcctggataa taggtaaggc ctgtcattgc ctgagggtcc ctaaatgccc atattccccc
    16261 ggtcgataca acaaaatcac tggttgcatt tcttaaatat ctccttcaac tgctcacgat
    16321 ccctcacgct gtgtggaaaa atgcccatcc ttcccttcag gagcatgcct attcccattt
    16381 tggcatcttc aaaaagattt ttcttcacta ctttgtagcg gagaatggcc tctatttcgt
    16441 ggagtctgcc atggcgtgca acgctatcca aaaatatctt atcaaaagtc cacgtatccg
    16501 tttctgctgc cttctgctcc ttaatggcta cttttcggat ggtatcgaag acgtgggcaa
    16561 tgtctatgtc gttcgggcac ctcacactgc acgtgatgca ctggacacag gtccatatcg
    16621 ctttagcttt gagtactttt tctcgctcac cgaggattat gtatcggata atgccatgag
    16681 gaaagatatc catctgagtg actaccggac aacctgctgt gcaccggtag cattggtagc
    16741 aagctgatat gttttgtcct gacaatgctt ccatttcttt taaaaatgtg agatcgttca
    16801 ttcgatatcc cgggttttcc tcatatttga acatccaagc atgagagctt tgctaacctg
    16861 cttactgcca tattataagt aataacccct tggcttacaa gtagactggg gttggcaagc
    16921 agtgttaacc gcttgttgat tacagcccgc attataatga agtgtacctt aaatggaaaa
    16981 acaggaaatg cgcattatgg cagaccttgt tccacgaaag cttatctgtc ttgtttctta
    17041 ttgatagaga aggttactca ccgcatcatt aaaggcgagt aagtgggaat attcacagag
    17101 cgttgacatt gccttactct cggtagaaat acctgcctct tcgactgcag caatggggtt
    17161 caaacctccc acgacaagca tgccaacctt atcaatcccc acaggaacat ccagcatggg
    17221 ctggtttggt ttgccaaaaa gaatacttcc tcccaatcct ttgtgggaca ttttttcttt
    17281 aaggacctca gcctctctga tgctcgccac cggtatctcg cgcatgcttg caagaatcct
    17341 cccgttattg ccggcaacag ctccggctac atcagttgct ccgcttttga tgagaatctc
    17401 cagcggatcc atggatgaac catcataact gataagggaa gtaaaccgct tggggacccc
    17461 tccttcaact tcaacaacac ctccatatct tgacgtaaca ggaagccctg atttaaggaa
    17521 aatgccgttc agcgtaatgc tgcacacagt cccaatgccc atacatcccc ggggtacctg
    17581 tacatcacca attctatccc ctccttttac caggataatc ctgttactca tcacgtatgg
    17641 tgatgcaaac accggggctg caattctaac tacgtctttc agttttttct ctgagaaaaa
    17701 agatatgttg agaataatgt tcccctcgag ggtgtcaaca ttgaaatctg tcagatagga
    17761 taagctgtca attttactga taacgaagtt gatcctctct gtcacgaaag cctttgtaac
    17821 ttcactcttt cctttttccg taatcattct ccccctctta ccctggactt tggtcatacc
    17881 catttgatcc agcagtttga ggtaatgacg cactgttctc tcagagagtt caattccgtg
    17941 gcttcgaagt tctcttgaca gctcaattga ccccacaata tccccatatt tatcgaggac
    18001 tcgcagaata gtaaacatgg ttctgttcat cacactactc cataagcaag cattgccctg
    18061 gctaccttca taaaaccggc aacgttcgct ccagttacat agtctccttt cctgccgtat
    18121 tcctcacttg cttcgagtgt tgctttatgg atgcctttca tgattttgag gagcagatca
    18181 tccacttcct gcctggtcca tgacattttt aagctgttct ggctcatttc aagccccgaa
    18241 actgctacac cgcctgcatt tgcagccttg cccggtccaa agagcacatt gttctcatga
    18301 aagatcctaa ctgcctcagg agttgacggc atattggctc cttcggctac acagatacag
    18361 ccgttcttga caagggcctc ggcatttttt ccatcaatct cattctgggt agcacagggt
    18421 atggcaacat caaccttaag cccctgttgc gtgatgacat cccagacact atcccctaaa
    18481 taacagacag agccgtattt atcagcatat tccttgattc tcccacgctt cacgtttttc
    18541 aattccatca caaaatcgca tttgtccccg catataccct cttcatcaac aatcgttgca
    18601 tttgaatcgc agagggttat aaccctacca cctaactgat tcaccttttc cattgcaaac
    18661 tgggcaacat tcccggaacc acttatagcc acggtcttcc cagtaaaact caatccccgt
    18721 gttgccagca tttcggcggc ataatagatg gcgccgtaac cagtagcttc cggtcgaatg
    18781 aggctgcccc cgtagtcgag gccttttccc gtaaaaaccc cggtgtgatt gttcattatt
    18841 ttcttgtaat aaccgaacat ataacctatt tctcttcccc caacaccaat atcaccggcc
    18901 ggcacatctg tttcagggcc aatatgtctg aaaagttctg ctgtaaaggc gtggcaaaac
    18961 ctcatcacct cattctctga ttttcctttc ggatcaaagt ccgaaccacc tttagcacca
    19021 cccatgggga gcgtggtaag cgagttttta aagatttgct caaaaccaag aaatttcatg
    19081 atgctgagat tcacagacgg atgaaaacga attcctccct tatacggccc tattgcattg
    19141 ttaaattgaa tccggaatcc acggttaacc cggacctcac ccctgtcatc aacccatggc
    19201 accctgaaaa caattgttct gtcaggttca acaatgcgct gaaaaatacc cgctttcaca
    19261 aactcaggat gtctttccgc tacgatctcc aaactctcca tgacttcaac aactgcctga
    19321 agaaattccg gctcttctgg atcccggtgt ttcacctgtg caatgatttc ttctatcaat
    19381 gacatggtat tatgcctcct tgaaaagtat taaaatccaa tttctttctc tgagttcttg
    19441 ccaagcccct ctgatgatgg gtgtgaaaac aaaccctatt caaataccaa cgaagtgatg
    19501 attaattatg cattttcccc cattttcttc aggacgcaca aaactttgct gatgagattc
    19561 acatattctc cgctaatgcc atgttcatca ttctacctgc cggataacat atcatgcacg
    19621 gcagttattg ccgtcttgag agcattacct gatcatgagt ttccatcact cattttcctc
    19681 tggacttgaa gatctcatac atgtagtaaa accaaaaatg gtgatcgata acaatcagag
    19741 aaaacctatt atttatatca gggtaaatac gatattattg gtaggtgatt cccctataat
    19801 caagtagaat tactatcaga ggctttctag ccagtaaact gcgtgtttta tcagttctct
    19861 tgcatttttt aaattcaact tttcttttat atttgcacgg taagtctcga ccgtcttgac
    19921 actcacacga agctcctctg caatctggcg cgtcccaaaa ccctggccta ccagtcggaa
    19981 tacctcgagc tcacggtcgc tcagaagctc ttccggagac gacgagcgct ttgatttccc
    20041 atcaacaaac ttttttacaa tacctgcgct gaccttttcg ctaatgtaga gatcacccct
    20101 tatcactcga cgaatcgcct ccatcatctt ttctgttgct tcctgtttca tgatgtatcc
    20161 ccgtgctccg gcccgcaagg cccgttctgc atatagtgat tcgtcgtgca tggaaatgat
    20221 gagcaccggc aggttcccgt atcgggcccg aatgttcttg gtcagttcta tgccgtccat
    20281 accttgaagc gagatatcga caatagtcag gtcgggcttc agtttcttga ggatttggag
    20341 tgcactctga gcgtcagagg cttccccgca ggttaacagg tcagcctcct gatcaataag
    20401 ttgggagagc cctttccgta caattggatg atcatctaca atgaaaatct tgtaggtctg
    20461 tccggaaaac tcgccctcct tcatcattgt cttatctttt ttttctgtca cgacgctcta
    20521 aaatgtacag ataaccgctg tctctccgtc tccattgctg cggatttcaa gggatgcatg
    20581 aattgaattg gccctgtacc gcatgatttt cattcccatt ccctcacaga aagtcttctt
    20641 gaaaccaatc ccatcatttc tgattgtcag tacagtctga tttttatcgt tgcggaaact
    20701 gatggtgata ttcctggcaa ggctatgttt gatggcgtta tttacagctt cctgggcaat
    20761 tcgatataag tgcatcgcgg ttaggtcatc atcgatagga accagctgat catattcaac
    20821 acaacatttt atcatgaaga gcttctccac ggtatctgcc aactcggaaa atgcagccat
    20881 tagtccctct cccactaacc attgcgggta gagtccccgt gcaagccctt tcgtttgtat
    20941 tattgcatca tcaattagct tcacgatctg gtttgcgtct tttgcttcct caaatgactt
    21001 tgcagcgatc tttctctcca gtgttttacc gagataagct attcctgcca gttgctggga
    21061 caggctatca tgaaggtctt gccctattct ttgcttttcc cattcagaga tttcgagaac
    21121 ctgcttttca agcttcctcc actcagtcat gtcacgaata atgccaagca cctcatccct
    21181 gccgcttacc gttaaaagga cttcataaca gtaggtcttt gagccatgac gatactggag
    21241 ctgaaatagc tgtgtggttc ccttctcaaa aacctgttca atatttgtca tgccccggcc
    21301 cgtaatatct ttagatagaa agggatactc atgtgccaag tccgcaattt tccttccaat
    21361 aatttttttg gctttaattt tctgtcccaa tgtttttccc ttcttaaaat cgagaatcgt
    21421 tccatccttt ttgattctga atataagatc aggtatagaa ttctgcaggg ctttatttct
    21481 gaccatactt tctttcaaat catcttcggc caattttctt tctgctattt ctcctatacg
    21541 ctttgcaagg gaatttatca gcttcctctc ctcttttaaa aacgggcctt cccagcactg
    21601 gggcctttct tcgagataga tcacctgaag aaaacctgat ttctcacctc ttacccgtat
    21661 atctgctgac tgtgtccatt ttgagggcct gtaattggca gtgataaatg tgttcgcgcc
    21721 cattgcaacc ttggccgccg caatctcagg atattgccat gcagaaggca taacctctac
    21781 catgtggtga agtattcttt cgagggaagt ccggtgtttc tcgacaagac tcgacagggc
    21841 atagatacaa ttcaactcct tgactctctc gcctaacgca cgggttttat tctgaaggct
    21901 ctccctggtc cgcctgtgct ctttctctga agctctgagg caagcgactt cttttcgcag
    21961 cttcgccaac tcatcggaat tgttcaattt gtcattttga gaatgtgcat ccatcttctc
    22021 agacgcatca accagatgtg cggataatgt gcagcattcc atactatacc atacatttgg
    22081 ggcggaattg aagcgttaca agacgtacgc ggtaaggact gacagcggca acgagtggga
    22141 gaagtaaaag aataagtgaa atgcttttca tttcattgct attttaaaat tcaccgctat
    22201 atttatgagt aagacgtatt aaaaatcaag gaggtaaata tgatcacttt cgaaatgaca
    22261 aaagacgaag caaacattgt ccagaatgtc atcgaacgct acctctatca tttgcaagta
    22321 gaaattatgc atacagataa aagagaattt cgagatgctt tgaaacaaag ggagaaattt
    22381 ctgaaggata ttatagacag gatgaaaaca aagatacttg ctgagccgta ggttctcttt
    22441 gcatcgttcg aaaaaggatc agagaaccat atccttgtta tgaggcactc caaagatatg
    22501 gatcaccccc tgtatctgtt ggttatgggc attcttcggt ccttgcccag tgcctttggc
    22561 gtaatcttga ttccaggagg ggattgccgt cgcttaaact cgctcccatc taccatcctc
    22621 gatacctgaa tgacaacatc ctttgcaaga aaagaagaag taatctcctc gggatgcttg
    22681 tcctcttcga cataggcctt gagaatggag tcaagtgttt catatggagg aagggtatcg
    22741 cgatcttttt gacctggcct gagttctgca gtcggttcct tatcaagaat ccgttgagga
    22801 attacaggac ctagggaatt cctgtaagca ctcaatgtat acacgagtgt ctttggtacg
    22861 tctttgatga ccgcaaagcc acccgccatg tctccataaa gggttgaata accgacactc
    22921 atttctgatt tattgccagc agccaataca agccagccga acttatttga aagggccatc
    22981 aggatattgc cgcgaatcct ggcctgaatg ttctcttccg taatgtcttc cttgcggttt
    23041 ttgaacaacg gcaaaagcgt tgaaatatag gccatgtata cgtcatcgat ggggactgta
    23101 agcaactcga tgccgagatt tgctgcgagt tctcgtgcat cttcctctga ttctttggat
    23161 gtatatcgtg atggcataaa aacaccagtg acatgatctt ttcccagggc atctgctgca
    23221 agggttgcaa cgattgccga atctattcct ccactcagac ccaaagtgac cttgtgaaaa
    23281 ccatttttcg caacatagtc atggaggcct agttcaagag ccttgtaaac ttcagcaact
    23341 ggattcagca tctctctctg atgccatgta atcgccggtt tatcggcata ttctacattc
    23401 tcggacagcg tgatcatttc aagtttcgtg gctccatctg tttctaactt ccctgtctgt
    23461 tcttttataa gctcgttcct ggggatatca atgaccagga ggtcttcact gaatgcagca
    23521 gcgcgtgcaa tgatagcccc ggtgttgtca caaaccatac tctggccatc aaaaacaagc
    23581 tcatcctgac cacctaccag gttcacgtag aagataaaaa ctctgttaat ggaagcatgg
    23641 tgactcacaa tttcttctct aacctttatt ttgcctgctt ggtatgggga ggtattgata
    23701 ttgagaatca aagcagcacc tgaagcagcc tgcgaagctg ttggcccttc atcaaaccat
    23761 gtgtactcgc aaatatttac tccaaaaatg agttcaccga gtttaaaaat aagggattca
    23821 tcgcctgaag aaaaataacg tttctcatca aatacgccgt aatttgacaa aagggtcttg
    23881 cggcagacac ctttaatttc atggtcatat atcactgaag ctgagttgta tattttgcca
    23941 ttgtctctat ccacaaaccc cacaaccgcc acaatatcct ttattgaccg ggcaacctct
    24001 tcaactgctc ttatgttatc ttcaataaac tttggcttca gaagcaggtc ttctggcgga
    24061 caacctgtta ttgcaagctc gggaaagaca acaatatctg cacccgaacc tcttgctttt
    24121 cgtgcatatt caataatctt tgcactgttg cctctaaagt ctccaacagt gcaattaatc
    24181 tgagcaatcg caattcgcca gttttcagtc atacatcatc ctgttcaaat gctaagaaac
    24241 acctcaaatc gcccagtgtc atttggacgc cataatccca aggcagacat aacatacaca
    24301 tttctgtcaa gattttttcc tcagttcaga tgaaaggata attgcttctg caatctcctt
    24361 catcgtcttg cgtaaatcca tacttttttt gtggatcttc ttataagcct cgtcttctga
    24421 aacatcgaga tccttcatca ggataccttt tgcacgctca acaatttttc gtgattcgag
    24481 ggcctctctt gctgccagta tctcttcgct gagatgggtg ttctcgatag caacagcagc
    24541 ttggttcgcc acagtttgga gaatgcttat ctcctccggc ttaaactggt gttctttttt
    24601 agtatagcaa tttatgactc caatagccct atctttcacc atcattggaa cagataacaa
    24661 agaaacgatc ccctcttttc gagccacttc aggatacatg tatccggact cctgtctaac
    24721 gtcagagact gtaacaggtt gtttttcaag aaccactctt ccgctcaccg actgtccaac
    24781 ttttaggttc ggtttattaa gatatccccg actgagactc tgggttgcag cgataaacaa
    24841 ttcgctgcgc ttttcatcga gaagcattat tgaacagatt tgcgaatcca tgacttttgc
    24901 tgtcatagta acaatcagct gaagtatttc tttcagatag tgattagata caatggttcg
    24961 cgagacttcc gataaaaggt ccaactgttt tgcctttctg ataacatcct cataaataat
    25021 cgcattgtgg atggcgcttc ccagatagcg actaacagta aatagaaggt ttacctggta
    25081 ttctgggtat ctgtgtacct ccatattccg aacattcata acccctatga cctcatcttt
    25141 ggatagaatg ggtatggaca tgaacgcctc atatttctct tcctcaaggg atgaaaaagt
    25201 tttaaaacgt tggtctttat atgcctcttt cggcaaaaca acaggccgtt tttcctttgc
    25261 agcccatcct gtgactccct ctcctatctt cagtttaacg cgcccaatgg atttttcttt
    25321 aggcttgctc gacgcagtaa ggataagctc atcatcctgg cggtcataaa gatagataag
    25381 acaagagtca gctgtaataa agttcatgat catagagatg atacggttaa gcagttcctt
    25441 caattcgagg ttgctgctga tgtccttcgc aacctcatgg aggattttca gttcctgctc
    25501 tttctgttct atcttttcac taagcttcac ttacctgccc cttgatatat ggaagcccgc
    25561 ggtattcgtt gcagtgatta agaatagtgt atccatgatt accttgtcaa taaaagatca
    25621 attcgagttg caggcatcca tgtttgtcaa tattagtact gttgattagg ttttccctgc
    25681 caaccattct ggcaactttc catttttcat agccaatcct ggtcaagttc atgtaactta
    25741 tgacatgtaa caccagtctg tccaatatgc aagcgaggta aacctgctaa aaccttctta
    25801 agttgcactt gggatgatcg gagaggtgaa caactactac ttaaaatagg aatctcaacg
    25861 gcagaaccct attggttttc tattcattta ccaggggttt ttgacgcgga aaaagaaaca
    25921 catttaaggg gctctgcagt aatctgttca aatgaactgc gggtattctc tcaagcttgt
    25981 catttctggc accgcatttg ctataatagt aaccggagtt ggggcatgca acaaaaaatg
    26041 agtctcgaaa aactaagggt aaatcttatt attattctta tcctcatctc atcggtcccg
    26101 ctggtactgt tcgctatcgt ggtttacagg aaagaggctg acctcgtaag gaagaaagtt
    26161 ctatcccacc tgaaaagcat agttgcccag aatagcctta tggttgatga attccgtctg
    26221 gaacgtatgc gcgatctcag cctgctggca gcaaccatcg aggcacatgg cggagaatgc
    26281 gacagaattc atcttaagct tatgcacaaa gaccgtacga tttacaaaca tatttttttc
    26341 gctgataagg agggaagaat caggtgtaaa acaggtaaag gacatctatt tgaaaggggg
    26401 cttgtcaatc aggcatggtt taaggctgca gcaaaaggaa agccttttat gggagaattg
    26461 acctctcgtg aaggaaaaga caggtatgac ctcctcctct cagtccctgt gaggatggct
    26521 aacggaacga caattggtgt agcaggtgct attgtcgatt ttgataatat cgccagtatt
    26581 atgaaaaata ctggtatagg gcagacaggc gagatttaca tcctgaacaa ggatggggtt
    26641 tttctcacgt caacacaagt ggaggagggc cgttcagggg agcgcatgga gcctgagcta
    26701 tttgcagggt atttcaaaga ggtagggcct aacgagtata tcgactatcg tggtaagaag
    26761 gttgtacggg tatttaaaaa actttcagat tctaactggt atctcgttgg agaacaggat
    26821 gcaacagagg tctttaagga cgttgcaaac cttagaaacc tttttgctgg ctttgtagtc
    26881 cttctcattg cccttataac ccttataggt tatctaattt caaggaaaat tgtccatctg
    26941 ctcaaatccg cctatgagca gaagaaagaa ttggaaatcc aggttatcca aaaagacaag
    27001 cttgcctcta tgggactact gactgctgga atcgcccatg agctaaacac acctcttgcg
    27061 agcgcccttc tgtacaccca gatgctgaaa gaagatgcca gaaacacatg gcccgtggcc
    27121 ctggaaaaac ttagttcaat tgaaggagaa attaaaagag gaagcaaaat tgtgaagaat
    27181 cttctcgact tttcaagaca gtcccagaca gataccgtga caacagatgt gaacgaggtt
    27241 ctcggcaaac tcctcgatat atctgagcag ttatgctctg acagaggcat tgagatcaaa
    27301 cgaacctttg aaaagaagct ccccctggta aaaggtaacg ctaacattct tcaccaggtt
    27361 ttcatgaaca ttgttgccaa tgctgtcgat gccatggaaa aaggaggcac tttgagcgtc
    27421 ctcacccggc atattgcttc attacacaaa actgttatag agattcagga tactggcacc
    27481 ggaattcccg aagagctgct gggcgatgtg ttcgaccctt tttttactac caaaccctcc
    27541 ggggagggaa ctggtttggg ccttgcaata agctattcca tggtgagaaa aatgggaggc
    27601 aacatcagag cgacaagcac ctgtgctact gaaaatagct atccctacca gccaaccgga
    27661 acaaccttca cgatagagct tccggcttta gatgatgaca cacaaagcac tgtaaaggaa
    27721 aggatggtaa agacacaatg aaaccctcac aaattctcat tgtagaagat gatcgaaaaa
    27781 tgaggctggc actgcgagaa atcatgacaa aggaagggta ctctgttgac acagtagaga
    27841 caggggaagc tgcgcttggt cgggtagaag aaacaagtta tgacctggta attacagacc
    27901 tcaaactgcc tggaatagat ggaatgagtc tgctcaaggc cattcgtaaa tcaagacctg
    27961 acacaagcgt ggtcattata acagcttatg caacggtaga tactgcagtt gaggcaatgc
    28021 gggaaggagc agaggactac atatcaaaac ctttcaacct tgatgaaata aggatcatcg
    28081 tgaaaaaagt gcttgaaaaa aaggcacttt tggatgataa caggctcctg agaaaccagc
    28141 tcaagaaaaa atacagctat gataatgtgg tgggtaatag cgaggctatg attgaggtct
    28201 acaaaaccat tgacaaggtg aaggatgcaa aagcaaccgt tctgatcttg ggcgagactg
    28261 ggacgggcaa ggagcttgtg gcaagagcta tccattacaa cagtacccgt tctggagaac
    28321 catttcttcc tgttaattgt gcggccctca cagagtctct ccttgaaagc gagctctttg
    28381 ggtacgtaaa aggtgcattt acaggggctc taaaggataa actgggtgtg ttcgaaacag
    28441 ctgatggtgg tacagttttc ctcgatgaaa tcggggacat aagccctcgc ctgcaacagg
    28501 tattgctccg ggttttggag aacggagaga ttcagccagt gggctcgaca gcacggaaaa
    28561 aggttgatgt cagggttgtt gcagctacaa acaaaaacct ggaagagtta atgaagacat
    28621 cattattcag agctgacctt tactatcgtc tcaacgtaat ctccctacgg ctgccctccc
    28681 tgttggagag aaaagaagat attccagccc ttgcaaacca ttttctcaga aagtactcgg
    28741 ctgaaaacaa caaaaccata accggcatat caaaggaggc gcttcagatg cttgaaggct
    28801 ataactggcc tgggaatgta cgggaactcg aaaatgtaat cgaacgggct acactccttg
    28861 aaacaacaga cgaaattacc gttgatagtc tctctgagaa aatacggagt tccctgaata
    28921 gatctccggt ggtacttgag gaggacctga ggactcttga ggaagtcggt agggcatata
    28981 taatagaagt ccttgagaag acaggcggaa acaaggttaa gacctccgag atcctgggta
    29041 ttaatcgtac ctctctctgg cggatgatgc agagattgaa aatcgttgca taatgcaaca
    29101 taggctgcaa aaagcaacac agttctggga atattttttc tgtatccccc gatttaacca
    29161 tacttctaag cgaaaataac tccacttacc tgtatttaat gtactttttc ttgggcatgt
    29221 tatttgcttt attaactgat atcgagccaa gggaggaggg aatgaatata cgttttattg
    29281 ataaacacag ggattttcaa attacttatg acagacttct tgagttgaaa aattcatttg
    29341 tccttgatca gcatcagcag ttcctttgta gagccctcag gcaagatgga aactggaggc
    29401 ttaaggaaag gattttggaa tttgccatag atatccgaaa gcccacagat gacttcattg
    29461 agaacatctg tgctgtcatg acaaacagaa acacatccat taatgcccga atccttgcag
    29521 ctgacgccct tcgagtcgtt gtgccaagga aggtgcggca aaccatggct tatcctacct
    29581 ttcggggggc atcagttttg cgggtcatga aagacattct caaggcacca gaggttcctg
    29641 agcttcacga agcagttacg cgagccttga aagctattca ggaaggatcg tgaagcaaaa
    29701 acgacagcag ggggtggacc atgacacata gagactacac accagaagtc acacgggtgc
    29761 caaccggcta caactgtgac ggtatgaaaa tcatcacata cttttccgat ggtaaagaga
    29821 ttgccaaaga aattctctgc gacaatgggc ttcagctcgt gatgacaggc accataccgg
    29881 atggcactgt attggagtat tattgggtgg gaaccctgca cagggttttc acatatgcaa
    29941 acaatagggc ccatggccgc agtcacacat tttaccctga tggtgcaatc tggatggaac
    30001 aagaattcat cgacggccta ctccatggcc ctatgaaaac attttacaaa ggcggcacga
    30061 tccaagaaga atgtacatac aaaagcggca gactccatgg ggagcttaaa aggtattatg
    30121 aagacggaac tcttgatact ctggcatact ttaatgaggg taagctggac ggagactact
    30181 gtacatactt tcagaacggg atgcccagag aaaaaagcat cttcagagat gggataagag
    30241 agggaaactc aatcaaatat tatgagacag gggagctaca gtgcatagat ctatgccttg
    30301 aagggagggt ggcccacaga aagagatttg atgagagagg aagattaatt tcagaccagt
    30361 cagagccagt tgctgagatt gaagaagaaa aaagcataga agcaaaggaa cacatgaaca
    30421 ggggcatgga cctagcaact atggggtgcc ataagcaagc tgcagaagaa ttccaacggg
    30481 ctatttccgc agatcctttc acctacgaag catacctgag gcttgctgtt gcctaccggc
    30541 gtctcggctt ttacggtgat tgcatcgata cgctcgggaa gttgctcgaa attaaccccc
    30601 accaccttga agcccgtttt aaccttgcaa tagcccatgt agtgacagga aacagggggg
    30661 aagcgcttgc tggctatcat gtattgagag acatagacga agggtatgca cacgggctaa
    30721 tgaccattct ggaatcgccc agattgcatc tgcaatgaag gatccaagct ggtatatata
    30781 tgaagacagt tattcttctg atctggtatg ggggccacca cttggtagct gctcatgggt
    30841 tataacatgg agtgcaaaaa acaggaaatg gatgcccata ccatgaataa aaaataccag
    30901 ttctatggca catggattgc tttacagcct acattaacac atttacacca tggggaggag
    30961 taacgtatgg aaaacgagaa ggatcggtgt gaacaggata gtttttctgg gttcattttt
    31021 aataagcccc agaggtttaa ggagagtttg cgagggtggg tcaaccattt tcataatatc
    31081 ggctgcaaat tcgtagtgct tcaacaagga gtcactggac agtatgtgat ttttaaaggg
    31141 agtcaggaca caagggagga tatggaccgg tcagtcccgt taagcaaagt gcttaataat
    31201 ggatatcgta tggtggagta tctcgatatg accgatgagg ttcgaaatca caagcacgac
    31261 accatggcat gaagtggtgt gcagcaccag ggatccatca ggggggttat ttagcgcaat
    31321 tgcctctcgt gaacaaatcg ctatgcatgt aaccaatgca gaaaggaggt tggctcgcag
    31381 gaaaatatat atcgaggagg taaatatgga aaccaccaga gacttttttc agggcacgga
    31441 agcggcacca attctaacca ggaatgacaa cgatacaacc cttacaaacc cggaacatat
    31501 tttttcattt ctttccttaa cacagaatcg tgctggcgac caacccatgt ctattgcacc
    31561 cttcaaattt cgttcggcgt gcaaacgggc acctgtcggc atgtcagtcg taaaaataga
    31621 cgatggcaag gtactgttaa gcaatccagc aatgcaaaac ctgttgggat actcaaggca
    31681 ggagttaata gggatgcctt tttcacagtt tactcatccc gaagacgtag agaaacagaa
    31741 agaggcctat ctgaaaatta agagtggaaa gcgtgtttcc ttcgacctca agaaacgata
    31801 tatccgcaag aatgggactt cttttactgc acatcttgta tccaccctca tccgtagatc
    31861 agatagcaaa ccccaggtta tgattgagat gatcatggaa gaggcataaa agtcacggca
    31921 gggagagaca gagctatgca attggatttg cgacctaagg aggtgtatgg gctatttccc
    31981 acagacatcc tttttcagac agcctactgg agtcaggtaa aatcccgcct gggctggaag
    32041 gcacttgcct tcgatttctc aacatcggga cacagtggcg atgtccttgt cctcgctaag
    32101 aatttcggga ataaattttc tgccgcctat gttccccagg gtccagaata cggtccagaa
    32161 cccgaagatt atggtcgttt tcttgaaacc atgtcttgtt cactggtaaa atatctcgac
    32221 ccaaaaccca ccttcatccg gtatgacctt ccctgggagt cccactatgc cagggatatc
    32281 gctcagggag agtactctgc tcctccagaa acgaggctgg tggagttgcg catgaatttc
    32341 ggaacacaaa catggaacct caggaaagcg gttaccaaca tgaccttcgc agatgcctat
    32401 gtggtcgaca tcacgccttc ggaagaagaa atcctccact ccatgaagcc caagacccgg
    32461 tacaatatac gccttgcaaa aaaaagaggg gtacatgtct ttccggcatc tgtggagatg
    32521 ttgcccaaat tctacgacct gtaccggcaa acagcaatgc gggatggttt ttatctgtgc
    32581 gggtataaca gtttccttgc acttttttcc cctatgccag aggaagccaa ttcaccagag
    32641 gttcattttc tcatcgctgc ccatgacaac gaccttcttg cctgtgccat catcactatc
    32701 agtggcaaaa cagcaacata cctctttggt gcgtcatcga gcgaaaaaag aaaccttatg
    32761 ggcccgtatg cagttcagtg ggctgccatt tgcctcgccc gctcaagagg atgccatgtc
    32821 tatgacatgg gggcaatctc acccacaaaa gatcctgccc atcctttctt cggactctac
    32881 cgattcaaat caggcttcgg aggcaggatt gtgcaccgga caggttcatg ggattaccca
    32941 ctggatcaaa atctatacaa tacgttcagg aactcagaga tgatgagtag agaataaacc
    33001 ctcaggaggg cgctatgaaa aacaaggcac gatacctata tatcaccgag catgacg
//
LOCUS       DAOOMC010000014        32836 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdus sp. isolate AMR_MDS_2685
            NODE_1130_length_32836_cov_6.056069, whole genome shotgun sequence.
ACCESSION   DAOOMC010000014 DAOOMC010000000
VERSION     DAOOMC010000014.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864760
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdus sp. (wastewater metagenome)
  ORGANISM  Syntrophorhabdus sp.
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae; Syntrophorhabdus.
REFERENCE   1  (bases 1 to 32836)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 32836)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,729
            Genes (coding)                    :: 2,708
            CDSs (with protein)               :: 2,708
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 7 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32836
                     /organism="Syntrophorhabdus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1130_length_32836_cov_6.056069"
                     /isolate="AMR_MDS_2685"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2699756"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..649)
                     /locus_tag="PK125_02480"
     CDS             complement(<1..649)
                     /locus_tag="PK125_02480"
                     /inference="COORDINATES: protein motif:HMM:NF012449.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0030955 - potassium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HPB37009.1"
                     /translation="MALPKNKTKIVCTIGPASNSIEVMKKMIEAGMNIARLNFSHGDR
                     KGHKQAIENLRLASKTVGTPIAIMADLPGPKMRIGQISPDPVHLTSGDLFTLTTRQIL
                     GSAGIASVTFDRLPDVVRPGNIIFLNDGLIQLEAIKVSDADIVCKVIVGGELRSRKGL
                     NIPNIDLGISAFTEHDHEWLQFAIENGVDAISQSFVETKSDIVLVREAAKACGRVP"
     gene            816..2474
                     /gene="pgm"
                     /locus_tag="PK125_02485"
     CDS             816..2474
                     /gene="pgm"
                     /locus_tag="PK125_02485"
                     /EC_number="5.4.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004614 - phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucomutase
                     (alpha-D-glucose-1,6-bisphosphate-dependent)"
                     /protein_id="HPB37010.1"
                     /translation="MKNSPLAGKLPPEEMLVDLKMLEREYYERKPDLKDPTQLVSFGT
                     SGHRGSSLSGTFNEAHILATTQAICDYRKSKGVDGPLYIGKDTHGLSGPGQRTALEVL
                     AANGVETMIQRDDGYTPTPVISHAILSHNKNRSEGLADGIVVTPSHNPPEDGGFKYNP
                     PNGGPADTQVTRWIQDRANLIMMAGNKEVKRMSYESALKASTTHSHDFVTLYVRDLIN
                     VVDMDCISSSGVAMGVDPLGGSSIQYWEPIKSLYKVNITVVNAMVDPRFSFVPVDHDG
                     KIRMDCSSPFAMAGLVSLKDRYQVSFANDTDADRHGIVTRSIGLMNPNHYLAVAIYYL
                     LTHRPQWPKEAAVGKTLVSSSIIDRVVATVGRRLTEVPVGFKWFVEGLFEGSCCFGGE
                     ESAGASFLKKDGTVWTTDKDGLIMNLLAAEITARTGKDPGEIYKELIEQLGYSWYTRV
                     DAPISPDEKAIMANLTAEMIVAKEIAGEPITEKLTKAPGNGASIGGLKILSSNGWFAV
                     RPSGTENIYKIYAESFKSESHLQTIVNEAQQIVKNALTGKAKGA"
     gene            2477..4120
                     /gene="pgi"
                     /locus_tag="PK125_02490"
     CDS             2477..4120
                     /gene="pgi"
                     /locus_tag="PK125_02490"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF001211.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HPB37011.1"
                     /translation="METGNLPLNKRPAWKALKTHYREIRRQHLRELFAIDPERGNYMC
                     AEGAGIFLDYSKNRVISETIRLLVQLAEESGLQSRIEAMFSGKKINVTENRAVLHVAL
                     RAPRGSSIIMDGKNVVPQVHEILDKMSDFSIKIRNGSWLGYTGKRIRNIINIGIGGSD
                     LGPAMAYEALKYYSDRNLQFRFVSNIDGTDFAEAVNGLNPTETLFIVSSKTFTTLETM
                     TNAHTARAWLLKGLGGEERSVAKHFVAVSTNKGTVEKFGINPENMFVFWDWVGGRYSL
                     DSAIGLSTMIAIGPENFHSLLDGFYQMDRHFRTAPFERNLPVLMGLLAIWYNNFFRAE
                     TMAVLPYEQYLKRFPAYLQQLAMESNGKQVTLDGARVVYQTGPIYWGEIGTNSQHSFY
                     QLIHQGTKLVPCDFIAFNKTLNPIGRHHDILIANVFAQSEALAFGKTSEEVKADGTQK
                     WLVPHKVFKGNRPSNTILADRLTPDTLGKLIALYEHNVFTQAALWNINAFDQWGVELG
                     KELAQRIIPELESIIEPALAHDSSTNSLIRQYRKRKHLQ"
     gene            4190..5353
                     /locus_tag="PK125_02495"
     CDS             4190..5353
                     /locus_tag="PK125_02495"
                     /inference="COORDINATES: protein motif:HMM:NF014460.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPB37012.1"
                     /translation="MSETGEPREIRNFGPELDFVKPDNDVLIVKLFGTWKIGSQVPGF
                     NRLTKELEKDSLIRKIRFDSSGLGRWDSILITFLLKIANYCEQKQIELTTEGLPEGVI
                     NLCKLASSVPERTAIRTSQIKLSLTTRVGIHVARIWGSIAETITFVGEIAIAFFRFLT
                     GRARFRWSDLLLIIQESGAQALPIVSLISLLVGLILAFVGSIQLKMFGAQIYIANLVA
                     IAMVRAMGAIMTGIIMAGRTGASFAARIGSMQVNEEIDALKTAGLPPMEFLVLPRIIA
                     LAVMMPLLTLYANLMGILGGMIVGVGGFDIGIREYLNATKGSLTLTTVGIGLFSSFIF
                     GILVATAGCLRGMQCSRSASAVGEATTSAVVTSIVSIIIATAIITIGCNILGI"
     gene            5381..6199
                     /locus_tag="PK125_02500"
     CDS             5381..6199
                     /locus_tag="PK125_02500"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HPB37013.1"
                     /translation="MIGEVQKQNKDKAVFEPIIVVEDLTMAFGDNVIQRDLTFTINRG
                     DIFIVMGGSGCGKSTLMRNLIGLQRPARGKVFYDKESFWDIEESERERILRRIGVMYQ
                     GGALWSSMTLAENIMLIIQEYQKIDHDQALELASLKLALVGLAGFEDYYPSEISGGMK
                     KRASIARAMALDPEILFLDEPSGGLDPISARMLDDLILQLKSSMGTTVVVITHELASI
                     FSIGNDSVFLDTESKTIIASGNPKKLLEECDNATVRRFLTRGASEPRIQAIGRA"
     gene            6222..7187
                     /locus_tag="PK125_02505"
     CDS             6222..7187
                     /locus_tag="PK125_02505"
                     /inference="COORDINATES: protein motif:HMM:NF014523.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HPB37014.1"
                     /translation="MSKPASKTLIGAFVLGAIALGVIAVIVFGSQKFFTKRSVYVMFF
                     EGSVKGLNEGSPVNFRGVKIGSVKDIELKFDPKDLIFLIPVYVEIDPTKVTGSKGTIG
                     SAEVWEELIQKGLRAQLELQSFVTGQLIINVDFFPGKPARYVGMDKRFPEIPTVSSPL
                     DQLLRTAEELPLKELFDKLLKSIEGIEKVANSPQIGSSLESLSEGLKESRKILGKIDQ
                     QIGPMMTNLKETSDSIKAFADKSQNVPGAVEKTLSTARASLEQAEKTFSSVQKLASDN
                     SALMYQIDNALNEVSKASRSVRSLSDYIYRHPESLIKGKRTLKGE"
     gene            7190..7825
                     /locus_tag="PK125_02510"
     CDS             7190..7825
                     /locus_tag="PK125_02510"
                     /inference="COORDINATES: protein motif:HMM:NF015823.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PqiC family protein"
                     /protein_id="HPB37015.1"
                     /translation="MKQKAALKTLVLGNILMALLLTACGSSPPARFYALTPMPPQISE
                     DKTILHDKNKIIAIGPIEIPEYLDRAEIVTRAKENQLMISEFDLWGGSIKTEISRVLI
                     ENIGSLLDRDGLAVVAWKTTMPESYRVPVILYRFDAVQDGSLTLRAQWAVFDKDGKSL
                     LSFQEAIVTKPAKGSGYDVLVALMSEALGDLSKAIAENLRFIKNNLVIKKF"
     gene            complement(8079..8261)
                     /locus_tag="PK125_02515"
     CDS             complement(8079..8261)
                     /locus_tag="PK125_02515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB37016.1"
                     /translation="MVEVVEEEVVVEEEEVVEVGFFQLVVEEEVVEEEVVEVDILQER
                     NIHHVLRMGQACSHLY"
     gene            8472..9221
                     /locus_tag="PK125_02520"
     CDS             8472..9221
                     /locus_tag="PK125_02520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB37017.1"
                     /translation="MAKNSKFLGFYALVFFLIPFSVLAVDADTRLSDQSGTLTGQNIE
                     IASLSVETQPVLDKPLPKRANFEQEEASQDARHVADWIVDSGNNRGMPFVIVDKIGAK
                     VFVFHADGRLRGAAPALLGMAVGDDAVPGIGNRKMSSIRPEERTTPAGRFVSYMGINF
                     YRKDILWVDYEGAMSLHRVVTNKPEERRLERLATPTPLDNRISYGCINVPATFFDKVV
                     KPAFAGVMGVVYVLPEVKSKKEVFASYYDVE"
     gene            complement(9247..9717)
                     /locus_tag="PK125_02525"
     CDS             complement(9247..9717)
                     /locus_tag="PK125_02525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB37018.1"
                     /translation="MKLSRYCYTHVLVFFLTGFAFMFSSCATTNHQQSTPYASAIQEI
                     IYGKISGLRESSSSRDTGPLGNTRWRVVNVSPNIQNFLKGTEFFFERSGVLVESAELP
                     NGTIYTDTHRYRVLDSTLVLTKDGLTAVTLWKIEGETLNIDRDDYRLTLKRINN"
     gene            10033..11784
                     /locus_tag="PK125_02530"
     CDS             10033..11784
                     /locus_tag="PK125_02530"
                     /inference="COORDINATES: protein motif:HMM:NF013283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex family protein"
                     /protein_id="HPB37019.1"
                     /translation="MTITGWIGLLHADQVKYLTVNVEGVTGKEREAVEKAMEIPQDLI
                     RDGIVDDSWLKRLERQGPQKVRQALQPFGYYTPDVAVKLESSAEGSYQFFISVKSGSP
                     VRIGAIRIVVQGPGAQEKVINDLIAEFPLHKGDRLRQDVYEEGKEVLLKKAISIGYLD
                     ATYVKHSVQVTLKKLSAEIELVLETGFKYFFGDITFTGESGFPESFLMRHLAFKKGEP
                     FSQEKIALTQVNLVSSDRFRLVNIMAKKDEAKDNFVPVEIALIPLKQKRVKFGIGYGT
                     DTRIRGQIRYQDFNILGTGQFFDMELKLSDIYQAIGARYIFPSRFDIKSLTSIKLGYE
                     HEKTSDKTVEFLALEGALTRAFGTGEKTASKERLGSIYLRLQQEDSRAGIEKTNVFLF
                     MPGVQFSHHQYDNVIRPTNGFRYNLELRGTDQFLGSETGFLQFLGRGEYLISLPQRVT
                     LLTRMQVGATTENEPAQDLPISVRFFAGGDTSVRGYRYQSLGPTDAFGNVVGGKHLLF
                     GSVELEKGIGKDWGIAVFYDIGNAFNSWRKIDFAKGTGIGGRYYTSIGPIRLDIARQI
                     GVRKPDYRIHLVVGIGL"
     gene            11781..15752
                     /locus_tag="PK125_02535"
     CDS             11781..15752
                     /locus_tag="PK125_02535"
                     /inference="COORDINATES: protein motif:HMM:NF016260.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translocation/assembly module TamB
                     domain-containing protein"
                     /protein_id="HPB37020.1"
                     /translation="MRKLYKICFIFSFIVLLLTGCFTWLLVTNQGAKFVINKVIQFIP
                     ADVQISSINGNIVRGLEVKNPTVRLISWEITAKRFYVRWNPFHILGGWIGVQVIGIEE
                     LSLNDLFPEVRNPHDLTWPKVKGILSWTKARVKLLYITSISYKEAGREIQRIDGLSAQ
                     LIWYLGRLNIRSFYSKIAGGVVDGSARANFTNPNFSAKFFMKSTRPYLGLDEYRFILN
                     LKAASRPLQMSGPMTIIGMSKNDEHVKLAGTVAISKKIISLDRIDFKEMGRPGSISGN
                     VSLDLSVAERPYELNLSLNDIDLLKNKEAMACISGILTAKGNISGYSGSFSIKNIAES
                     WKNLTLEGQLKGNNQELKIFEIKGRALKGSVTGKITASWSHALKVSGMLNARNLDPAL
                     ITPDWPGIVNADFSSELTFTRSSYPEGKMKANFLRSTVRNRPLTGNIEVQWAKGLLSV
                     DQAELHGKGFDVKAHGILQRKVDYNAKITDLGGLIPSATGRLTASGWFRWANDRWGGI
                     VTAEGYTIGIGGLKADSALVSIKVNEKEGDVLKANLQAGNVNYGPINLRSALVTIGGK
                     LSNHDVFISLKGPKSNGEIAASGGYHEGEWSGTLSKMEGWDALAGVFNVLRPVALNIS
                     RDRAAMSPMILSGASGELAEISGALTFNPIRGSFSIRWEKLRLERANQIYGGIKIGGT
                     GSGFLQGQIFDKERMRLVGSGTNTFSIARGPITLHGSTVSKIDCGENGIRASFDVGFA
                     NGGKFESQFVSNEPAYLRMPESGEMRAMWRDIDVTILKPLSPHDIDITGKVSGTVQGR
                     ISAGSHFEISGKTDVTDSSITWKGEGGVITSTAEYASVHFNWKDEVLKGRVDVKFPSH
                     GKVNGNFDLPISAHFPVKIARTSAVNMQLCGEIREKGIISSIFPGFVEESKGHLIFDV
                     TRKGTWDLPDVEGQIKLRDATAYLPGTGIRVEGVTANATFVQDRIELRSFTGKSGSGK
                     LEGSGTFWFKGFGIARFKTLLTGEGFQTIYLPELQVSISPDLIIEGEGDKMMIKGKIL
                     IPDALLRDVGNKTSVRTSDDVVIVDAPRKEKKPLKSDIEVQVSVVMGKKVHVQVSGLD
                     GQIQGNVNLTGRLPDKLLGKGMLKIAGGKYNSYGIKLDVVRGNIMFDNKPVDLALLDI
                     MAIRTFNPGKFDEIKAGVTVTGTPLSPLIKLYSDPPMTDTDVLSYLVLGRPTKAGAET
                     QQTTLLLKSASAVLGVSKSGGIQDQIQHLLGVDTLEVQEGANRSFTTSRTSTVTGSTL
                     NNSLMTVGKYLSPDLYISYGRSLFSDQYLVSARYSLSKKLELESKAGMETSVDLFYKI
                     EFD"
     gene            complement(15808..16542)
                     /locus_tag="PK125_02540"
     CDS             complement(15808..16542)
                     /locus_tag="PK125_02540"
                     /inference="COORDINATES: protein motif:HMM:NF027851.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="general secretion pathway protein GspB"
                     /protein_id="HPB37021.1"
                     /translation="MSYILEALKKLERDRQRERMPNLLTVQEDHKPATKKRTLWPYIV
                     IGLILVNVVFVFLLFRVDPWTNTVVSTRYKNLSKVETATPAPSTAIDEKSGQLPMEPR
                     KDITITENKDLVQPSPLKTRSDLPKKTPHEPSPTKTEPLQPSPPREANVEKPSKAIKP
                     ALTGGKILSIGELPADTRAGLPELKMTVHSYNEHMQSRFVVINNSILREGQSINADLK
                     VEQITQNGVVLNSRGYRFILKINENL"
     gene            complement(16549..17472)
                     /locus_tag="PK125_02545"
     CDS             complement(16549..17472)
                     /locus_tag="PK125_02545"
                     /inference="COORDINATES: protein motif:HMM:NF024589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HPB37022.1"
                     /translation="MYREYFGLKETPFSIAPDPHYFYISEGHREALAHLIYGINSDGG
                     FILLTGEVGTGKTTVCRCLLEQIPENCEVAFILNPTLSSVELLAAICDEFRIAHAKGN
                     ETTKDLVADIYDFLLHIHETGRRAILIIEEAQNLTVQVLEQIRLLTNLETSQRKLLQI
                     IMLGQPELRKTLAKPQLRQLSQRVTAQYHLGPLGKHEIDSYVKHRLAVAGLTHREVFP
                     PSVIRMLYNLTRGIPRIINLICDRALLGAYTEGKAKVDKSTLAKAAREVSVRGKNRWQ
                     VRKIYIWLTIAILIALTIAGFLQLALTLKGS"
     gene            17661..17915
                     /locus_tag="PK125_02550"
     CDS             17661..17915
                     /locus_tag="PK125_02550"
                     /inference="COORDINATES: protein motif:HMM:NF008448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajD family HNH nuclease"
                     /protein_id="HPB37023.1"
                     /translation="MKESAVPKNDYRERSLNIHGLICAKCAREFDYKDRHLLTVHHKD
                     GNHRNNPPDGSNWENLCIYCHDEEHTRGLLGDYFSEGKYP"
     gene            complement(18173..18679)
                     /locus_tag="PK125_02555"
     CDS             complement(18173..18679)
                     /locus_tag="PK125_02555"
                     /inference="COORDINATES: protein motif:HMM:NF024986.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HPB37024.1"
                     /translation="MGNFIFYFSSTLFLAFFSLSPSFAFNEKDLARLLQSNECKFCNL
                     HSANLSGANLSGSQLSNSVLSHANLSGANLSRANLSGAFLTNANLSGANLADAFMVDA
                     NLSDANLSDTNLAGANLSGATWTDRQKCGKDSYGECKRTTLEGAPSSPEGSRSRTPDT
                     PAWLPGGR"
     gene            18803..18967
                     /locus_tag="PK125_02560"
     CDS             18803..18967
                     /locus_tag="PK125_02560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB37025.1"
                     /translation="MLTRKRGKRKLKGDALFEIKKSYWVCNRKDLINMPEIEKCLKLL
                     KVRYSEKLKI"
     gene            complement(19101..19175)
                     /locus_tag="PK125_02565"
     tRNA            complement(19101..19175)
                     /locus_tag="PK125_02565"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19139..19141),aa:Arg,seq:acg)
     gene            complement(19321..20430)
                     /gene="rodA"
                     /locus_tag="PK125_02570"
     CDS             complement(19321..20430)
                     /gene="rodA"
                     /locus_tag="PK125_02570"
                     /inference="COORDINATES: protein motif:HMM:TIGR02210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HPB37026.1"
                     /translation="MVKVDKRKLYHLDWYLIINGLALFAIGIMNLVSATSSFYSGSYN
                     FIIKQLIALAIGIVIISIIINFDYRAIAANAKLFYFLSLVSIVLVLVWGMAAGGARRW
                     ISIFGISFQPSEFIKPVMVFMLAYLLHEKKRDKILLGLKDILIPLAWVVVPVVFIVFQ
                     PDLGTGVIIFLVFLAMIWFVGLKKSTYATLLSAGIVLPLIAWQYVLRPYQKQRIYTFL
                     NIDSDPLGVGYHTKQAIIAVGSGKFFGKGYLQGTQHKLQFIPEHHTDFIYTVFAEEWG
                     FFGSILFFLLFITFINRCMKISQQAYDELGSIIAFGLGSIIFFQFAINVLMNIHLAPV
                     VGIPLPFISYGGSSLISVLISVGLLLNINMRRYMF"
     gene            complement(20414..22300)
                     /gene="mrdA"
                     /locus_tag="PK125_02575"
     CDS             complement(20414..22300)
                     /gene="mrdA"
                     /locus_tag="PK125_02575"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR03423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HPB37027.1"
                     /translation="MKKKKDNHSTNDMFYAEPRGTEYEKVMKEKTVPDKYKWCKWVLI
                     MTMIVLSIRLWHLQIMQGNEMRQQSEMNRIRIKKVLAPRGIMFDRMGKILADTRPSFN
                     LYITPEDIKDFNQTVDGLSGLMGIDREEIIEKLKAASNFPSSFPVKIKDDVTMDELAK
                     VEANRVYLPGVSIQIEPKRNYPYGKVLAHTLGYVSEISSEELKEEKYRTYSPGDYIGK
                     YGLERMYEDYLRGIDGEKRVEVDAMGREVRTLEMKDPIPGSNMYLNINLDVQMVVEKA
                     LEGKRGGCIALEPKTGAVIALASRPAFDPNKFASGITKKDWQAIALDSTHPLQNRVIQ
                     GRYPPGSTFKILTALKALEDGLINANTSYGCRGGFPFGNRIFKCWKKGGHGTVNVHRG
                     IVESCDVFFYNIGLRLGVDRIHSFGTSVGLGKPSGIDLPSEARGLLPSTEWKRKTQGV
                     PWYEGETVSVAIGQGAVWLTPVQLVQLSSFVANEGITFKPQIVSKIIGPDGKTQKIFE
                     PVVTADIKMKKQVFTIVKEGMQGVVNEKSGTAYGSRIENVHMSGKTGTAQTTPVKGRD
                     LGDHAWFIAYAPSEDPAVAISVLIEYGGHGSSEAAPVAKAISESLLTVKPEIKEAMVR
                     GQGR"
     gene            complement(22297..22794)
                     /gene="mreD"
                     /locus_tag="PK125_02580"
     CDS             complement(22297..22794)
                     /gene="mreD"
                     /locus_tag="PK125_02580"
                     /inference="COORDINATES: protein motif:HMM:NF016019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HPB37028.1"
                     /translation="MTKIAVYLVIALVLTTFESAVVNFLPIDFFNPDVGIPFVIYTTF
                     FLGPQAGLITSVVIGLIQEIVSTSPSGSLLFTKASIFIISVFMRNKIYIDSKYSFSFV
                     CSGAVIVEASLFLALSYLTRGESRDMFNVAFYSIPNAIFTGLLSLFLFSLIGFLNVKY
                     FNKPQ"
     gene            complement(22791..23591)
                     /gene="mreC"
                     /locus_tag="PK125_02585"
     CDS             complement(22791..23591)
                     /gene="mreC"
                     /locus_tag="PK125_02585"
                     /inference="COORDINATES: protein motif:HMM:TIGR00219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HPB37029.1"
                     /translation="MKNTVVIIIAIAVLVVSFFIFTNTPLFLRGYSVAKTGFGEVSGP
                     LLRTVGKPVELVSYIFKNYIGLIRTNRENTELRNKVASLQMENQKISELEYENKRLKS
                     ILNLMEQRPKTMIAARVIGEDIKNWFKCIILDKGKSSGITIKMPVITPRGLVGQVVET
                     DRWVTKVMVINDTNSSVDVYVEGRNTRGILEGTGQTLLRLKYIRKNDEIEIGDKLITS
                     GKDGIYPKGLATGIVTTINRGKPGIFSEIEVMPFNNFNILEEVLVVKK"
     gene            complement(23604..24644)
                     /locus_tag="PK125_02590"
     CDS             complement(23604..24644)
                     /locus_tag="PK125_02590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007115517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HPB37030.1"
                     /translation="MFESLFGFISKDLAIDLGTANTLVYVKGRGIVSNEPSVVAIHKD
                     AKGGKKVIAVGEEAKKMLGKTPGNIVAIRPLKDGVIADFEITEAMLKYFIQKIHNKKS
                     YARPRIVISVPSGITPVEKRAVKESAESAGAREVFLIEEPMAAAIGVGLPITEPNGNM
                     IVDIGGGTTEVAVISLAGIVYCNSVRVAGDKIDEAIIQYVKRKYNLLIGERTAEMIKI
                     NIGSAYPSQDGEELTMEVKGRDLVGGIPKTLEISSKEIREAIAEPVNAIVEAVRIALE
                     RTPPELASDIVDKGIVISGGGALLRNLDLLIKEVTRLPVIIAENPLSAVVEGTGKALD
                     EITLLKEIATYF"
     gene            24740..26131
                     /locus_tag="PK125_02595"
     CDS             24740..26131
                     /locus_tag="PK125_02595"
                     /inference="COORDINATES: protein motif:HMM:NF025010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HPB37031.1"
                     /translation="MLKFMRKYATGYLVKAMFGLIIIVFVFWGVGSFRERDRIVAEVG
                     PYKVSIMEYQEEYNRTMNMYRMIYKDRLDENMLRELKVKEKAMNDIVDKYILLIKAGE
                     LGISVSNSEFREHLESIEMFKRDGKFSEMAYKEILKRNGLDPKRFEQSEKNAILNTKM
                     INLIRDTGALMSEGNLWNSYVKEKGRINLGYTEVDPASYRTKVTVGDQEVADTYEKEK
                     NIRRGDNIYKLKYILFDEKSSVKDDVAYLELLKSKDLEAYGKQKGLQVADLGSMKEGD
                     LLKRLKNLKADEWLRGMKKGEISLPVRADAKSYIFQVVDIEEGKPIDRTLAMKEIKDR
                     LINEKAKNFAKNVAQELINKKTVDSKKDTGFVPRTTLAIPKIGQIPKDDLGVLSLSKE
                     FPIYNKPVEIDGIFYVFHFKEEKLPDKDQWEKDKESYKRYALSKSQSDFLKSFMEGLR
                     KKEKVKIEWKEIS"
     gene            26135..27130
                     /gene="tsaD"
                     /locus_tag="PK125_02600"
     CDS             26135..27130
                     /gene="tsaD"
                     /locus_tag="PK125_02600"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HPB37032.1"
                     /translation="MIILGIDTSCDDTSFAVLRNGRELLANLVSSQIDLHSVFGGVVP
                     EIASRKHVELVDQLYREVLQNANLSGKSIQAIGVTSGPGLIGSVLMGLCFAKGLSLSL
                     GIPIIGVNHIEAHAMSILLEYDVEFPFIALVVSGGHTIILRIDDFGLYKVLGSTRDDA
                     AGEAFDKIAKFLGLGYPGGRIIEKMSKEGKPDHVFFPRPMIDDNNYDFSFSGLKTSMI
                     NYIKKYNVNNENISDILSSFQEAAFDVLANKTLRAAKNIGIPRIVVGGGVASNGRLRA
                     IFHERCSKDRIDVFFSSPEFCTDNGAMVAKVAHHYYVTNKTSPIDIAGYSRMKFH"
     gene            27134..27910
                     /gene="rsmA"
                     /locus_tag="PK125_02605"
     CDS             27134..27910
                     /gene="rsmA"
                     /locus_tag="PK125_02605"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: protein motif:HMM:NF012616.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HPB37033.1"
                     /translation="MLKKRLSQHLIKDRNITDKMVRLAQLKEDDIVVEIGAGHGDLTK
                     SIAEKAGHVYAVEFDKDMIARLKTLAKKYGNITVIQEDFLKTSLLPFSADKNIIIMGN
                     IPYKITGPIIFKLLEDRKIVSAAYLTMQAEVAGRIVSRSHKRTYGAISVVCQILCDVK
                     ILFNLQASVFVPPPKVDSAFLRMVPKEEKKNIDRELLGFVRASFQNKRKYLKYALSKT
                     YTEDMIERIYSFMGLSDTARAEEISPDQFEKMYNFVKGNG"
     gene            27903..28919
                     /locus_tag="PK125_02610"
     CDS             27903..28919
                     /locus_tag="PK125_02610"
                     /inference="COORDINATES: protein motif:HMM:NF017828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacyl-CoA dehydratase"
                     /protein_id="HPB37034.1"
                     /translation="MVEVSILQKGAVIGFTTTIPVEVIFAGGYTPCDLNNIFVTDSNP
                     MQYIEKAERDGFPKSMCNWIKGIYGVVMKHPVDAVITVMQGDCSNTQALTEILRYKGI
                     RTIPFSFPFDRDRQVLQREIEKLKKALSVKDEQLKAVEEGMSKARSALGVIDRMTWEK
                     GLVSGYDNHLWLVRASDMLGDYNRYNSMARDFIEEVKSNKPKEGVRIGCMGVPPIIPD
                     LHDFIESVGGHVVYNETQRQFILPFKVMNLTDRYLKYTYPYGIFSRLEDIQKEIDRRE
                     IQGIIHYVQAFCYRTVEDVILRETLGIPMITIEGDLPKPLDSRTKLRIEAFIELLSNA
                     GDAA"
     gene            28916..29173
                     /locus_tag="PK125_02615"
     CDS             28916..29173
                     /locus_tag="PK125_02615"
                     /inference="COORDINATES: protein motif:HMM:NF027581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4911 domain-containing protein"
                     /protein_id="HPB37035.1"
                     /translation="MKSFVHQTPQEPVEKTRKLIIERNKIGFFKAILESYEDVAIFTV
                     LDGKSGLIELIYSTCFEEEISAIVQDMIHCGIAIKEATDGE"
     gene            29163..30542
                     /locus_tag="PK125_02620"
     CDS             29163..30542
                     /locus_tag="PK125_02620"
                     /inference="COORDINATES: protein motif:HMM:NF039907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HPB37036.1"
                     /translation="MVNKENILRKLLDGNGLYGDIFSEKRTYTHIQLESDRIEKLERG
                     LDEGVGLRLINPWKMYFASTNNMDEGNLLDMAGTLSRFSREKSKSQQKKIQKQNGFYP
                     FTIIKDPEGIDIDEKLTMVKKIGTMAKKMEPRIRQVRVVYRDTHQTTYIYTSDGKDFK
                     DNRTQVVLSLLLVGEEKEEIQTSYEAIGGFYGYEFFTDEVVEQFVLKTVKRLSGLLSA
                     KEAPMGTKTVVLSSEAGGTMIHEAIGHGLEADLAMEGLSCYKGMLGDSIASPLINVVD
                     DATVPNLRGTYAFDDEGTESQRTVLVENGVLKNFLFDKFYAMKYGKNSTGNGRRESYR
                     YKPIPRMSNTMILPGSEDPAAIVSSVDDGVLVVKMGGGQVDTVRGDFVFEISEGYIIE
                     KGRVGEMIKNATIMGNGLKVLHEIDMVGNDLGYGIGTCGKDGQGVPVSDAQPTLRIPE
                     IVVGGKSAG"
     gene            30713..31264
                     /locus_tag="PK125_02625"
     CDS             30713..31264
                     /locus_tag="PK125_02625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB37037.1"
                     /translation="MKRTIGFYFAMLFFVMFITTPLFSATEDDLVGTWSMYHSAKLKV
                     SRIGTTTSQNNSIVTLKGDGTFTMVENDPPDTYTYTGNWQLIKKGKKVLISFDQGGRD
                     ELIRLLEDWMMEVASGYGVPISDINISLAKLTISQPSIPKKTNIPKTTTIKATGLVSA
                     YLDGMFITRKFSYDSKVSFICRQ"
     gene            31330..32127
                     /locus_tag="PK125_02630"
     CDS             31330..32127
                     /locus_tag="PK125_02630"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HPB37038.1"
                     /translation="MTKEVDWLELWRELVSRKLHPETESLVQKYRAHGQKHNERPDSL
                     LDFVLKKIDPEDTAVDIGAGTGRWTIPLARKTRNVTAIEPVSGMVAMLKENLDYANIK
                     NVKILLQTWEDAALPVHDTAVCAHGMYGSPDLAEFVRKMERLARKGCYLAMRLPPADG
                     ILSELSLQIYGCRHDSPDAMIAYNALYTMGIDVNILVETSIVNWFNQTMEDAFIRAKR
                     HLRIQMSDTTYDDLIHSTLKRRLNFSDGAYIWPDGMRSALLWWTPVH"
     gene            32174..>32836
                     /locus_tag="PK125_02635"
     CDS             32174..>32836
                     /locus_tag="PK125_02635"
                     /inference="COORDINATES: protein motif:HMM:NF015949.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF364 domain-containing protein"
                     /protein_id="HPB37039.1"
                     /translation="MMKPASEAGDILKETMEAIQSSLGNEMETITVEKTVIGLFFTGV
                     KLDTGEGGLCFTPVKSIPEAVCCPSSAQFMPASGKLEGRKATRFLNDMASGGPLKKAV
                     AIAVMNALSSICWKRQNPETYRIRIGVDPIDEMVIPDDGFVVVVGALVTALKKLKQRG
                     KPFAVLELDPSILKGDELEYFVPFAEAYKVVPKADILVITGTTLINNTLETLLEKKKP
                     GAE"
BASE COUNT         9703 a         6611 c         7576 g         8946 t
ORIGIN      
        1 aaggaacgcg tccacatgct ttggcagctt cccgcaccaa cacgatatca gatttcgttt
       61 ctacaaaaga ctggctgatg gcatccactc cattttcgat tgcaaattgg agccactcat
      121 ggtcatgctc tgtaaatgca cttatgccaa gatcgatatt agggatgttc aagcctttac
      181 gggatcggag ctctccacca acaatgacct tacacacaat atctgcatcg gatactttta
      241 ttgcttcgag ctgaataaga ccatcgttta aaaaaataat attacctggc ctaacaacgt
      301 ccgggagccg gtcaaatgtc actgatgcaa tgcctgcact gcccaatatt tgacgcgttg
      361 ttaatgtgaa caaatcccct gaggtgagat gtaccggatc aggagaaatc tgtccaatgc
      421 gcattttagg acccggcagg tcagccatga tggcgatggg tgtaccaact gttttggatg
      481 ccaatctgag gttttcaatg gcttgtttgt gtcctttgcg gtcaccgtgg gaaaaattca
      541 gccttgctat attcatccca gcttctatca tttttttcat aacctctata gaattagagg
      601 caggccctat ggtacagaca atttttgtct tatttttcgg aagcgccatg tttatccaac
      661 ctccctgcaa ttaaaagttc tatcactttc tgtttttaaa gacaatgatt acgaatttct
      721 caagctcatt ttaatcgtga acatggcatc cctgctacat tgtatgatag acttctcaca
      781 atcagcttaa ttataaaaag aaaggagttg ccggcatgaa aaactctccc cttgcaggta
      841 aattaccgcc agaagaaatg cttgtagatt tgaaaatgct tgaaagagag tactacgaac
      901 gtaagccgga tttaaaagat cctactcaac ttgtaagctt tggaacaagt gggcacaggg
      961 gatcatcgct ttccggcaca ttcaacgagg cccatattct ggcaacaacc caggccattt
     1021 gtgattacag gaaaagcaaa ggggttgatg gcccgctcta cattggcaaa gatactcacg
     1081 gattatcagg gcctggacaa cgaacggctc ttgaagtgct agcagccaat ggagtggaaa
     1141 ctatgatcca gcgtgatgac ggatataccc cgaccccggt aatttcccac gccattcttt
     1201 cccataacaa gaaccggagt gagggcctgg cagatggtat tgtcgtaacc ccttctcata
     1261 accccccgga agatggaggt tttaaatata atccaccaaa cggaggtcca gctgataccc
     1321 aggtgacccg atggatacag gaccgggcga atcttattat gatggctggc aacaaagaag
     1381 tgaagaggat gtcctatgag tcagccctta aagcctctac aacgcactct cacgatttcg
     1441 tgactctcta tgtaagagac ctcatcaatg ttgttgatat ggactgcata agctcctcag
     1501 gtgttgccat gggtgtcgat ccccttggag ggtcttcaat ccaatattgg gagccgatta
     1561 aatccctgta caaagtgaat attactgttg ttaacgcaat ggtcgatcca aggtttagct
     1621 ttgtgcccgt tgatcatgac ggaaaaataa ggatggattg ttcaagcccc tttgccatgg
     1681 caggcctggt cagccttaaa gacagatacc aggtttcttt tgccaatgat actgatgctg
     1741 accggcatgg gattgttact cgctcaattg gcctcatgaa tcccaaccac tatcttgcgg
     1801 tagcaattta ttacctgttg acacataggc ctcagtggcc aaaagaggcg gctgttggaa
     1861 aaacgctggt cagtagcagt atcattgacc gtgttgttgc aaccgtgggt agaagattga
     1921 ccgaggttcc tgtgggcttc aaatggtttg tcgagggttt gttcgaaggt tcatgctgtt
     1981 ttggcggtga agaaagtgct ggtgcaagct ttctgaaaaa agacggaact gtctggacaa
     2041 ctgacaaaga cggccttatc atgaatctac ttgcagcaga aattacagcc cgcacaggta
     2101 aagacccggg agaaatctat aaagaactaa tagagcaatt gggatattcc tggtatacga
     2161 gggttgatgc accgatatca cctgatgaga aagccatcat ggcaaacctg actgcagaaa
     2221 tgattgttgc aaaagagatt gcaggagaac ctatcactga aaagctgacg aaagccccgg
     2281 gcaatggcgc atcaataggt ggattgaaaa tcctgtcatc aaatggctgg ttcgcggtca
     2341 gaccatcagg aacagagaat atatacaaga tttacgcgga gagcttcaag agtgagagcc
     2401 atctccagac tattgtaaat gaagcgcagc agatagttaa aaacgcttta accggaaaag
     2461 caaaaggagc atgagcatgg aaacgggcaa tctacccttg aacaaaagac cggcatggaa
     2521 agcgcttaaa actcattaca gagaaatacg aaggcaacat cttcgtgagc tttttgccat
     2581 tgatcctgag cggggtaatt acatgtgtgc tgaaggcgca ggaatctttc ttgattactc
     2641 taaaaatcgt gtaatcagcg aaactatcag gcttcttgtt caattagccg aggaatccgg
     2701 cctgcaaagc cgcattgaag ccatgtttag cggtaaaaag atcaatgtta cagaaaatcg
     2761 tgctgttctt catgttgcgc tgcgggcacc aagaggatca tcaataataa tggacggaaa
     2821 aaacgtggtg ccgcaggtcc atgaaatcct cgacaagatg tctgattttt ctatcaaaat
     2881 acgcaatggc tcatggctgg ggtacaccgg aaagcgaatt cgcaatatta tcaatattgg
     2941 tatcggtggc tcagacctgg ggcctgccat ggcttatgag gcacttaaat attatagtga
     3001 tcgaaacctg caattcaggt tcgtttcaaa tatagatggt acagactttg ccgaggcagt
     3061 aaacggtctt aatccaacag aaaccctttt tattgtttct tccaagactt tcacaaccct
     3121 ggaaacaatg acaaacgcgc atacagcgag agcctggttg ttaaaagggc ttggcggtga
     3181 agaaagatca gtggcaaaac actttgttgc agtatcaaca aataaaggga cagttgaaaa
     3241 attcggcatc aatcccgaga acatgtttgt tttctgggat tgggttggtg gccgttactc
     3301 tttggattca gctatcggtc tttcaacaat gatcgcaatt ggccctgaaa acttccattc
     3361 actccttgat ggtttctacc agatggacag acattttcgc actgccccgt ttgagcgaaa
     3421 cctgccggta cttatggggc tcttggctat ctggtacaac aactttttta gagcagagac
     3481 tatggcagtt ctcccttacg aacagtatct caaacgtttc ccagcctatc ttcaacagtt
     3541 ggccatggaa agtaacggca aacaagtaac ccttgacggg gcaagggttg tttatcagac
     3601 cggcccaatc tattggggtg aaataggaac gaacagtcag cactctttct atcagctcat
     3661 acaccaaggt actaaacttg tcccctgtga ctttattgct ttcaacaaga ctctgaatcc
     3721 tattggtcgt catcacgata tactcattgc caacgttttt gcacaaagtg aggccctggc
     3781 ttttgggaaa acgtcagaag aggttaaagc tgatggaaca cagaaatggc ttgtaccaca
     3841 caaggttttt aaagggaaca ggccttcgaa cactattttg gctgacaggc ttacacctga
     3901 tacactcgga aagcttattg ccctttatga gcacaatgtt tttacccagg ctgctttgtg
     3961 gaacattaat gcatttgatc agtggggggt agagcttgga aaagaattgg cacagcgaat
     4021 cattcctgaa cttgaaagca taattgagcc tgcacttgcc catgatagtt caacaaatag
     4081 cctgattcgt caatacagga aacgaaagca cctacaataa ctctttgtct gatgcaatat
     4141 gttgacttgt gatatgattt accctatcat tcatcatcgg tagacacaaa tgagtgagac
     4201 aggtgagccg agagaaatta gaaattttgg cccagagctc gactttgtaa aaccagacaa
     4261 tgatgtcctt attgtgaagt tattcgggac ctggaagata gggagtcagg ttccgggttt
     4321 taacaggctt accaaggaat tggaaaaaga ctcacttata aggaaaatca ggtttgattc
     4381 cagtggtctt ggccgctggg atagcatcct tatcacattt ttattaaaaa tagccaacta
     4441 ttgcgaacag aaacagattg agttgacaac agaaggtttg ccggaaggtg tgattaatct
     4501 atgcaagctt gcatcatctg ttcctgagag aacagcgatc agaacaagtc aaattaagct
     4561 ttcactgact acacgagttg gaattcatgt tgcgagaatc tggggatcaa tagctgaaac
     4621 aattactttt gtcggtgaga tagcaatagc ttttttcagg tttcttactg gtagagcacg
     4681 atttcgatgg tcagatcttt tacttataat acaggaatca ggggctcagg cccttcccat
     4741 tgtttcgctt atcagccttc ttgtcgggtt aatacttgct tttgtgggtt ccattcaatt
     4801 gaagatgttt ggagctcaaa tctacatagc aaatttggtt gcaatcgcta tggtgagggc
     4861 catgggagcc attatgacag gtattattat ggctggtcga acaggagctt cttttgcggc
     4921 cagaataggg tccatgcagg tgaatgaaga aatagatgcc ctgaaaactg ccggattgcc
     4981 accgatggag tttcttgtac tgccaagaat tatcgccctc gctgttatga tgcccctcct
     5041 tacattatat gctaacctca tgggcattct tgggggcatg attgttggtg tgggagggtt
     5101 tgatattgga atccgagagt atctcaatgc aacaaaaggt tctttaacgc tcactacagt
     5161 tggtataggg ttgttcagca gttttatttt tggtatcctt gtggctacag cgggatgcct
     5221 cagaggaatg cagtgcagta gaagcgcatc agcagttgga gaggctacta cttcagcggt
     5281 agtaacgagc attgtgagta tcattattgc tacagcaatt atcacgatag gatgcaacat
     5341 cttgggtata taaaagacag ttaatggtta acggtgatca gtgattggcg aagttcaaaa
     5401 acagaacaaa gataaggcgg tctttgaacc aattatcgtg gttgaagatc ttaccatggc
     5461 atttggtgac aatgttatac agagggatct tacatttacc ataaacagag gggatatttt
     5521 tattgtaatg ggggggagtg gttgcggcaa aagcacttta atgaggaacc tcataggtct
     5581 ccaacgtcct gccagaggaa aggtatttta cgacaaagaa agtttctggg acattgaaga
     5641 atcagaaaga gaaagaatct taaggcgcat aggcgttatg tatcagggag gggctctttg
     5701 gagttccatg actcttgcag agaacataat gctgatcatt caggaatatc aaaaaattga
     5761 tcatgatcag gctctggaac tcgcttcgtt aaaactggct ctcgtaggac ttgcaggctt
     5821 cgaagattac tacccttctg aaataagtgg tggaatgaag aaaagggcga gtatagccag
     5881 ggcaatggca ttagatccgg aaattctttt tcttgatgaa ccctcaggag gattggatcc
     5941 tatcagtgcc cggatgcttg atgatctcat tttgcaactc aaaagtagta tgggaacaac
     6001 agtagtcgtt atcacccatg aacttgcaag tatttttagc atagggaatg attcggtttt
     6061 tctcgatacg gaatcaaaga caattattgc atcaggtaat cccaagaaac ttcttgaaga
     6121 atgtgataac gctactgtaa gaagatttct cacgcgaggt gcctcagagc ctcgtattca
     6181 agccattggc cgagcctaat ggatgtggtg aaggagattg tatgagcaaa cctgcaagca
     6241 aaacattgat tggagccttt gtgctcggag caatagcatt gggggttatt gcagtgatag
     6301 tcttcggttc tcagaaattt tttaccaaga ggtctgttta tgtaatgttt tttgaggggt
     6361 cggttaaagg gctgaatgag ggttcacctg tcaactttcg gggggtgaaa ataggttctg
     6421 tcaaggacat tgagctaaaa tttgatccaa aagacctgat ttttttaatc ccggtgtatg
     6481 ttgaaattga tcctactaaa gtaactggtt ctaagggaac tatcgggagt gctgaagtct
     6541 gggaagaact tattcagaaa ggactgagag cacagcttga gttacagagt tttgtaacag
     6601 gccaactcat aataaatgtt gattttttcc caggcaaacc agcacgatat gtggggatgg
     6661 ataaaaggtt tcctgagatt cctacagtct catcccccct cgatcaactt ttgagaacag
     6721 cggaagaact tccactcaag gaactctttg ataagttatt gaaatcgata gagggaatag
     6781 aaaaagttgc taactcaccc caaatcggat cgagccttga atctttatct gaaggtctga
     6841 aggaatcgcg caaaattctc ggaaaaatag accagcaaat tggccccatg atgactaacc
     6901 tgaaagaaac ttccgattca atcaaggcct ttgccgataa gtctcaaaat gtccctggtg
     6961 cagtggagaa gacgctttcc acggcacgtg cctctttgga acaggcagag aaaactttta
     7021 gttctgttca aaagcttgca tcagataatt cagcccttat gtatcagata gacaatgctc
     7081 tgaacgaagt atccaaagcc tctcggtctg ttcgatcttt atctgattac atctacaggc
     7141 atcctgaatc cctcattaaa ggcaaaagaa ccttaaaagg agaatgagta tgaaacagaa
     7201 ggctgctctc aaaacgcttg tactggggaa catccttatg gcccttcttt taaccgcttg
     7261 tggatcatct ccaccagcaa ggttttatgc gcttacacct atgcctccac aaatatcaga
     7321 agacaagaca atactccatg acaagaacaa aattatagcc ataggaccca ttgagattcc
     7381 agagtatctt gacagagcag agattgtgac acgggccaag gagaatcaac tcatgatttc
     7441 ggaattcgac ctgtggggtg gttccataaa aacggagatc tcgagagtgc ttattgaaaa
     7501 tatcggatcc ttgcttgacc gcgatggcct tgctgtagtt gcatggaaaa ctaccatgcc
     7561 agaatcttac agggttccag taattttata tcgattcgat gctgttcaag acggcagttt
     7621 gacccttaga gcacaatggg cagtgtttga taaggatgga aaaagtcttt tgtctttcca
     7681 ggaagcaatt gttactaaac ctgcaaaagg gagcggatac gatgtcctgg ttgctttaat
     7741 gagcgaagcc ctgggggatt tgagtaaagc catagctgaa aacctcagat ttattaaaaa
     7801 taatttggtc attaaaaagt tttaagaaaa tattgctttt atcgaatagt aggatataat
     7861 gtaatgtaaa gtctttttta taaaatacac ccaggcacac tagggagagg taatgaagcc
     7921 tgggaaaaaa ctaaaagaag gaggatgtat gaacaaaaaa tctttagttg catttctgtt
     7981 tcttgttgcc gttggtttat tgttttcagg ttgctgccag ataatgggct ctttaaaacc
     8041 agctccggca ggcccatgtg ttccatatga aggctgcttt agtaaagatg actgcaagcc
     8101 tggcccatgc gtagaacgtg atggatgttt ctttcctgta aaatgtccac ctccaccacc
     8161 tcctcctcca ccacctcctc ctccaccacc agctgaaaaa atccaacctc caccacctcc
     8221 tcctcctcca ccaccacctc ctcctccacc acctccacca ccaccaccac ctaagaaaga
     8281 ccgcaactaa agcttcatag gtgaattgcc ccgctgattg ttaaaaaaca tgaggcgggg
     8341 cctcttctat cagttggtaa aagagaatgc atactgttac ttaatgggta attacatggg
     8401 agccaaaaga aaaaccaact tgagaatttg tgattgctgc gatattgata atttttcaaa
     8461 ggaatttgtg cgtggcaaag aatagcaaat ttttgggctt ttacgcgctg gtcttttttt
     8521 taatcccttt ttctgttttg gcagtagatg ccgatacgcg attgtcagat cagagcggaa
     8581 cattgactgg gcaaaacatt gaaatagcaa gtctgtctgt cgaaacacaa ccagtgttgg
     8641 acaagcccct gccaaaaaga gcaaactttg aacaggaaga ggcatctcaa gatgcgcgac
     8701 acgtggcaga ctggattgtg gactcgggta acaatcgcgg aatgcccttt gtaatcgttg
     8761 acaagatagg cgctaaggtt tttgtattcc atgctgatgg tcggcttcgc ggggcagccc
     8821 ccgcactgtt aggaatggct gttggagatg atgcagtacc gggtattggt aacaggaaga
     8881 tgtcttcaat ccgtccagaa gaacgtacaa cacctgcggg tcgtttcgtt tcttacatgg
     8941 gcatcaattt ctatagaaag gatattctct gggttgatta tgagggtgct atgtcgcttc
     9001 atcgggtagt tacaaataaa ccggaggaac gccgcttgga acgcctggcc actccaacac
     9061 ccctcgacaa ccgtatctct tatggatgta tcaatgtgcc agcaacattt tttgataaag
     9121 tcgtgaaacc agcttttgcc ggggtgatgg gagttgttta tgtactgcct gaagtaaagt
     9181 caaaaaaaga ggtcttcgca tcatattatg atgtcgaatg agtgtaaaca cctgtagaaa
     9241 aaggctttaa ttatttatcc ttttcagtgt caatctatag tcatccctgt caatgttcaa
     9301 agtctcacct tctattttcc agagtgtaac agcagtaagc ccatccttgg taagaacaag
     9361 ggtggaatcg agtacgcgat accgatgggt atctgtatag atggtaccgt ttggtagttc
     9421 tgcagattca acaagaacac cacttctttc aaaaaagaat tccgtgcctt ttaaaaaatt
     9481 ctgaatgttt ggactgacgt tcacaacgcg ccatctggtg tttccgagtg gtccagtatc
     9541 cctgctcgat gaagattctc ttaaaccaga gattttgcca tatattattt cctggatagc
     9601 gcttgcatag ggagtagact gttggtggtt agttgtagcg caagaagaga acataaaagc
     9661 aaaaccggtc agaaagaaaa ccaatacgtg ggtataacaa tatcttgata acttcataag
     9721 cactccttca aaagagttga tgatgtgaag cctaatcacg atgtctgatg ataataaact
     9781 ctttgggtta cgatttcaag agattccaac ggaaaggccc ctttattgat ccatttgcag
     9841 ttaaatgcat tcatcgctta gctttgcgca agaggtgttt ggctgggata gtgtcccata
     9901 aagaactggt tgtgttaata tctgaagaat tgatttcaca tgaaaacgct gtgtattata
     9961 tcacacacct gaatgctgca cagagcgcat acaaagacag tattgccagt gattcgactc
    10021 atagtgttat gcgtgaccat aaccggttgg atagggttat tacatgctga ccaagttaaa
    10081 tacctgacag ttaatgtgga aggggtaaca ggcaaggaac gtgaagccgt tgagaaagcg
    10141 atggagattc cacaagactt gatacgagat ggtattgtgg atgatagctg gctaaagcgc
    10201 cttgagcggc aggggcccca aaaagttcgg caagccctcc aaccttttgg atattataca
    10261 ccggatgtgg ctgtaaagct tgagtcatca gctgaaggct cctaccagtt ttttatatct
    10321 gtcaaaagtg gaagcccagt gcgaatagga gctattcgaa ttgttgtgca gggtcctggt
    10381 gcgcaggaaa aggtaatcaa tgatcttatt gccgaatttc cattacacaa aggggacagg
    10441 cttcgacagg atgtatatga agaaggtaaa gaggttctcc taaagaaagc aatcagtatc
    10501 ggttatctgg atgcgacgta tgtgaaacat tcagtacaag taactttaaa aaagttatca
    10561 gcagagattg agctggtttt agaaaccggg tttaagtatt tttttggtga tattactttt
    10621 actggcgaat ctggtttccc tgaatctttt cttatgagac accttgcatt caaaaaggga
    10681 gaaccatttt cacaggaaaa aatagcattg acccaggtaa atctggtcag ttcggaccgc
    10741 ttcagacttg tcaatataat ggcaaaaaaa gacgaagcaa aagataattt tgtacctgta
    10801 gaaattgctt taattccgtt aaaacaaaaa cgagtaaaat ttggtattgg ttatggaact
    10861 gacacaagga tcagagggca gattcgttat caggatttca atatcctggg cacagggcaa
    10921 ttctttgata tggaattgaa attgagtgat atttatcagg ctattggtgc ccgctatatc
    10981 tttccaagca ggtttgatat caaaagtcta acgtctataa aacttggtta tgaacacgaa
    11041 aagacttctg ataaaacggt tgagtttctt gctcttgagg gcgccctgac aagggctttt
    11101 ggaacggggg aaaagactgc gagcaaagaa agattaggtt caatctattt gagattgcaa
    11161 caggaggact caagggctgg tattgaaaaa acaaatgtat ttcttttcat gccaggtgtt
    11221 cagttttccc atcaccaata tgataatgtt atacggccta cgaatggatt cagatataac
    11281 cttgaattga gaggcacaga tcagttcctg ggatcagaaa ccggtttttt gcagttcctt
    11341 ggaagaggag aatacctcat atctctcccc caaagagtca cccttttaac gaggatgcag
    11401 gttggagcaa ctacagaaaa tgagcctgct caggaccttc ccatatctgt tcgttttttc
    11461 gccgggggcg ataccagcgt tcgcggatac aggtaccaat cgcttggacc gacagatgca
    11521 tttggaaatg ttgttggagg caagcatttg ctttttggaa gtgtggaact ggagaagggc
    11581 atagggaaag actggggtat agctgtattc tacgacatcg ggaatgcttt taattcatgg
    11641 aggaaaatcg attttgctaa aggcacaggg ataggtggtc gttactatac atctatcggg
    11701 cccataaggt tggatatagc aaggcagatt ggagtaagaa agccagatta tcggattcac
    11761 cttgttgtgg ggatcgggct atgagaaaac tctataaaat atgttttatc ttttctttca
    11821 ttgttttgct gttgacagga tgttttacat ggcttcttgt taccaaccag ggagccaaat
    11881 ttgtcattaa caaagtgata caattcattc cggctgatgt ccaaatcagc agtatcaacg
    11941 gtaatattgt tcgggggcta gaggttaaaa atcccaccgt gaggctgata tcctgggaaa
    12001 tcacagccaa acgtttctat gtccgttgga atccctttca cattttagga ggatggattg
    12061 gagttcaggt gattgggatc gaggagctct ctctcaatga tttattcccc gaagtaagaa
    12121 atcctcatga tttaacgtgg cccaaggtaa aagggatatt gtcatggacc aaggcaaggg
    12181 tcaagctgtt gtatattaca agtattagct acaaagaagc aggacgtgaa atacagcgca
    12241 ttgatggcct tagtgcccaa ctcatatggt atcttggaag attaaacatt cgatcgtttt
    12301 actcgaagat agcaggcggg gttgtggacg gttcagcacg ggcaaacttt acaaatccga
    12361 atttttcagc aaagtttttt atgaaatcta cccgaccata tcttggtctt gatgagtatc
    12421 gctttatatt gaaccttaag gcagcttcac gtcccctgca aatgagtgga ccaatgacca
    12481 tcatcggcat gtcaaaaaat gatgaacacg taaagttggc gggcacagtg gccatttcaa
    12541 aaaaaataat aagtcttgat agaatcgact tcaaagaaat ggggcggcca ggatcgatca
    12601 gtggaaatgt atcgttggat ctatctgttg cggaaagacc atacgagctg aatctcagct
    12661 tgaatgatat agacctatta aagaacaagg aggcaatggc ttgtatttcg ggaattctga
    12721 cagcaaaggg taatatatcc ggctatagtg gttctttcag tataaaaaac attgctgaat
    12781 cctggaaaaa ccttaccctt gaaggacaac tcaaaggcaa taaccaagaa ctaaaaatct
    12841 tcgagattaa gggtagggca ttaaagggtt ccgtcactgg taaaatcact gcctcatggt
    12901 cacatgcact taaagtctca ggtatgttga atgcaagaaa tcttgatcct gcccttatca
    12961 cgcctgattg gcccgggatc gttaacgctg atttttcttc tgaactcacc tttacacggt
    13021 cctcatatcc agaaggcaaa atgaaggcaa attttctcag aagtacagta cgaaacaggc
    13081 ctttgaccgg gaatattgaa gtacaatggg ccaaaggtct gctttctgta gaccaggcag
    13141 aacttcatgg aaagggattt gatgttaagg cacacggaat actgcaaagg aaagtggatt
    13201 acaatgcaaa aattactgat ctcggtggtc tgattcccag tgcaaccgga cgtttaacag
    13261 catctggctg gtttcgatgg gccaatgatc gatggggagg aatcgtaaca gccgagggtt
    13321 acaccatagg catcggaggg ttgaaagccg attccgcgtt agtaagcatc aaagtgaatg
    13381 agaaagaagg tgatgttcta aaagcgaatt tgcaggcagg caatgtcaat tacggtccca
    13441 tcaaccttcg atcagcgctg gtcacgatag gtggaaaact ctcaaatcat gacgtattta
    13501 tctcacttaa agggccaaaa agtaatggtg aaattgcggc aagcggggga taccatgaag
    13561 gggaatggag tggaaccctt tctaaaatgg aagggtggga cgctcttgcc ggagtcttca
    13621 atgttttaag acccgttgcc ctaaatattt cacgagaccg agcagctatg tccccaatga
    13681 ttctttcagg cgcctcgggc gaattggccg aaatttcagg agccctgaca tttaatccca
    13741 taagggggag cttcagtata cgatgggaga aactcagact ggagagggca aatcagattt
    13801 atggtggcat caaaataggc ggaactggat ccgggttttt acaaggtcaa atttttgata
    13861 aggaacgcat gagattggtt gggtcaggga caaacacttt cagtatagcc agaggaccta
    13921 taacgctcca tggttcaaca gtatcaaaga tcgattgcgg tgaaaatggt atcagagcct
    13981 catttgatgt gggttttgcc aatgggggaa aatttgagag ccagttcgtt tcaaatgagc
    14041 ctgcttattt acgaatgcct gaatctgggg aaatgagagc aatgtggcgt gacattgatg
    14101 ttaccatcct taagccattg tcaccacacg acatagatat caccggcaag gtatctggta
    14161 ctgttcaggg aaggatcagt gcaggctctc attttgagat atcaggcaaa acagatgtga
    14221 cagatagttc catcacgtgg aaaggtgagg ggggagtaat aacttctaca gcagaatatg
    14281 caagcgttca tttcaattgg aaagatgaag tgctcaaagg aagagtcgat gtcaaatttc
    14341 ccagccatgg aaaagtaaat ggtaattttg atcttcctat atcagcgcat ttcccggtga
    14401 aaattgcaag gacgagtgcg gtcaacatgc agttatgcgg tgagattcgt gaaaaaggta
    14461 ttatcagttc aatctttccg ggttttgttg aagaaagcaa aggccaccta atttttgatg
    14521 tcacccgtaa aggtacatgg gatttgcctg atgttgaagg gcaaataaag ctcagagacg
    14581 ccactgctta cttaccaggg acagggatca gggttgaggg ggttaccgcg aatgctacat
    14641 ttgtccagga tcgaatcgaa ctacggtcat tcacagggaa atccgggtca ggtaagttag
    14701 aaggttcagg aaccttctgg tttaaaggtt ttggcattgc caggtttaag acattactca
    14761 caggagaagg ttttcaaaca atctatttac ctgaactcca agtatctatc agccctgacc
    14821 ttatcattga gggggagggc gacaagatga tgatcaaagg taagatcctt atacctgatg
    14881 ccctcttaag agatgtcggc aataagacat ctgtccgtac aagcgatgat gtcgtcattg
    14941 ttgatgcccc acgaaaagag aaaaagccac tcaaaagcga catcgaagtg caggtttctg
    15001 tggtaatggg taaaaaagtc catgttcagg tttcaggcct cgatggtcag atacagggaa
    15061 atgtgaacct tacaggacga cttccagaca aattgctcgg aaaaggaatg ctgaaaattg
    15121 cgggtggtaa atataatagc tatggaatca agttagatgt ggtacggggg aatatcatgt
    15181 ttgacaataa acctgtggat cttgccttac tggatataat ggctatccga acgttcaatc
    15241 caggaaagtt tgacgaaata aaagcagggg tcacagttac aggaacaccc ttatcccccc
    15301 tgattaagct ttattctgat cctcctatga cagacacgga tgtcctgtct tatctggtcc
    15361 ttggcaggcc aactaaggcg ggtgctgaaa cgcaacaaac taccttactc ctgaaaagcg
    15421 caagcgcggt tcttggcgtt tcaaaatccg gcggaatcca ggaccagata cagcatctgc
    15481 ttggagttga taccttagag gtgcaggaag gtgcaaaccg ttcatttacc acttcaagaa
    15541 catcaacagt cacaggatct acccttaata attctcttat gactgtagga aagtatctct
    15601 caccagacct gtacatctca tatgggcgtt ccctgtttag cgaccagtat cttgtatctg
    15661 cacgttacag tctgtcaaaa aagctggaat tggaaagcaa agctggcatg gaaacaagtg
    15721 tcgatctttt ttacaaaatc gaatttgact gaatctgtgt gaaagggttt taacagcaaa
    15781 ccaggtcgca gattataagt aaattactca aaggttttca ttaattttca ggatgaaacg
    15841 atagcctcgg gaattaagga ctacaccgtt ttgggtaatc tgttctacct tcaggtcagc
    15901 atttatggac tggccttctc ttaagatact attattgatg accacaaatc tcgactgcat
    15961 gtgttcatta tatgagtgca ctgtcatttt gagttcggga agaccggctc tcgtgtcggc
    16021 aggcagctct ccgatactca gtatttttcc acctgtcaaa gcaggtttga tagcctttga
    16081 aggtttttct acgttcgctt cacgcggagg cgatggttgt agaggctccg tctttgttgg
    16141 agatggctca tggggtgttt tttttggtaa atctgaccga gtcttgagcg gtgagggctg
    16201 taccaagtcc ttattctccg tgattgttat gtcttttctg ggttccattg gtagttgccc
    16261 acttttttca tcaattgcag tcgatggcgc tggagttgcc gtctcaacct ttgacaggtt
    16321 tttatatctg gttgagacga cggtatttgt ccacggatca accctgaaca gcaagaaaac
    16381 aaacactaca ttaacaagaa tgagaccgat aacgatgtaa ggccatagag ttctcttctt
    16441 ggttgcaggt ttgtgatctt cctgaacggt taagaggttc ggcatccgtt ctcgttggcg
    16501 atccctctca agtttcttaa gtgcctcaag gatatatgac actttcagtt aactcccttt
    16561 cagtgttagc gctaactgta aaaagccagc tatcgtcagt gcaatcaaaa tggcgatggt
    16621 aagccagata tagatctttc tcacttgcca cctgttttta cctcttacag agacctctct
    16681 tgctgcttta gcaagggtac tcttgtcaac ctttgccttc ccttctgtat atgccccgag
    16741 aagtgctctg tcgcatatta aattaatgat cctcggtatt cctctcgtca ggttgtaaag
    16801 cattcggata acagaaggcg gaaaaacttc tctgtgtgtg agccctgcca cagccagacg
    16861 gtgctttaca tatgaatcta tttcatgctt tcccaatgga ccgagatggt actgggctgt
    16921 gaccctctgg gaaagttgac gtaactgagg cttggcaagg gtttttctca gttcaggctg
    16981 tcctaacatt atgatttgaa gcagtttacg ctggctggtt tcgagattag tgagtaagcg
    17041 tatctgttcg agaacttgta cggtcaggtt ttgtgcctct tctatgataa gaatcgccct
    17101 tctgcctgtt tcgtggatat ggagaagaaa atcatagata tctgccacaa gatctttagt
    17161 agtctcgtta ccctttgcgt gggcaatcct gaattcatcg catatcgctg caagaagttc
    17221 cacggatgac aaggttggat tcaaaataaa agcaacctcg cagttttcag gtatttgttc
    17281 cagtaagcat cggcacacag ttgtcttccc tgtgccaacc tcaccagtaa gtaaaatgaa
    17341 tccaccatcg ctgttgattc cataaatcag atgggctaag gcttcacggt gaccttcact
    17401 gatgtaaaag tagtgaggat caggagcaat agaaaaaggt gtttctttta acccaaaata
    17461 ttctctgtac ataacactca acgttcatta aagattaata tatgaacaac gggaaagcaa
    17521 aattttttaa actgagctat acttagaggg cgaatatttt attgaggagt atacatgaga
    17581 cggaaatcat tttcagtaaa gaaaagaaga tggcctgaaa aaaagatagt ggtaaggacg
    17641 ttgatgagat tgtacgagaa ttgaaagaaa gtgcagtacc aaaaaacgat tacagagaac
    17701 ggtccttgaa cattcacggg ctcatttgcg caaaatgcgc gagagagttt gattataagg
    17761 acaggcatct tctcacggtt catcacaagg atggaaacca taggaacaat ccaccagatg
    17821 gatcaaattg ggagaacctt tgtatttatt gtcatgatga ggagcatacc aggggacttc
    17881 tcggtgatta tttcagcgaa gggaagtacc catagggact ggtcattaat ggggaaggcc
    17941 aaactctttc agcgagtggc tgggtgaaat catataagag aagagatccc ccgggacagt
    18001 gcaaggatga atcggggggg atcagcgcac cgtgaacccc ggggggagtg gacatcgtgt
    18061 taacgatata atatatcaca gttgacaaca aaattcacta tagcacaaaa tataatcagc
    18121 ctggaaaagg ggaaaccctt cagtttgaga gctaaagaag tggagttatg agttatcttc
    18181 ctcccgggag ccaggcaggt gtatcaggtg ttctactgcg tgaaccctcg gggctgcttg
    18241 gtgcgccttc aagggttgtt cttttgcact caccatagga atccttgccg catttctgcc
    18301 tatcagtcca ggtagcacct gacagatttg cacctgccag gttagtgtcg gacagatttg
    18361 catcagagag gtttgcatca accataaagg catcagcaag attggcacct gagaggttgg
    18421 cgttggtgag aaatgctcct gataggttgg cacgtgatag attggccccg gaaaggttag
    18481 catgggaaag gacagagttg gacaattgag aaccagagag gtttgcacct gatagattcg
    18541 cactatgaag gttgcaaaac ttgcactcat tagactgcaa cagtcgggcg aggtcctttt
    18601 cattgaaagc gaaggaaggg cttaaagaaa aaaaggcgag gaaaagggtg gagctgaaat
    18661 aaaaaatgaa attacccatc tttagatttt actcagaata atcattattt acaaaaatat
    18721 gtaaaccatg cattaacaaa aaatacttat acaagctggc aattataaaa aacatgtaaa
    18781 aatataaccc gttacttgga gaatgttaac acgaaaaaga ggaaaaagga agctgaaagg
    18841 tgatgctctt ttcgagataa agaagagcta ttgggtatgt aaccggaagg accttatcaa
    18901 tatgcctgaa atagagaagt gtttaaaact gttaaaggtt cgttattctg agaagttaaa
    18961 gatttaattc tatggatttt ttagttaaca tatttgaaat catgaataga agaaaaaggg
    19021 aaattttgtc gatattgact ataggttaac gataagattt ataatttctt taaaattgct
    19081 tttaagttat tgaatttatt cgcgcccagc aggattcgaa cctgcaagcc tttggattcg
    19141 tagtccaacg ctctatccat ttgagctatg ggcgcaggtg aatgtcgaac cggattatat
    19201 catattcaaa aggtttctgc gaagcttttc ttgatcatca tgttatcagg gctgtgaacc
    19261 gattgccaaa aagacgattc agatttacct gacccctcgc tactttttgg caccttcgaa
    19321 ctaaaacata tacctccgca tgttgatgtt taaaagcagg ccaacagaga taagtacaga
    19381 aataagtgat gacccaccgt aactgataaa gggcagagga attcccacaa caggtgcaag
    19441 atgaatattc attaaaacat ttatagcaaa ctgaaagaaa atgattgagc caagcccaaa
    19501 ggcgataatt gaacccagtt catcatacgc ttgttgtgag atcttcatgc aacgattaat
    19561 gaaggtgatg aacaataaaa aaaacagaat cgacccgaaa aatccccatt cttctgcaaa
    19621 aacagtataa atgaaatcag tgtgatgttc aggtataaac tgaagcttat gttgagttcc
    19681 ttgtaaataa ccttttccaa agaacttacc tgaaccgaca gcaataatag cttgtttcgt
    19741 atggtaacca actcctaacg ggtctgaatc aatattgagg aaggtataga tcctttgttt
    19801 ttgatatggc cttaggacat attgccatgc aatgagtggt agcacaattc cagcgctcaa
    19861 tagggttgca tatgtcgatt ttttcagacc tacgaaccag atcattgcaa gaaaaaccag
    19921 gaagataata acacctgtcc caaggtccgg ctgaaacacg atgaagacca caggaacaac
    19981 aacccatgca agtggtatga gaatgtcttt tagtccaagg agtattttat cccgcttttt
    20041 ttcgtgaagc agatatgcga gcataaaaac catgaccggc ttaatgaact ctgatggctg
    20101 aaagcttata ccaaaaatgc tgatccatct tcgtgcccca cctgctgcca tgccccatac
    20161 aagaacaagg acgattgata ccaggctgag aaaatagaaa agctttgcat tggcggctat
    20221 cgccctatag tcaaagttta taatgatgct gatgatgaca atcccaattg ccagagcaat
    20281 aagttgtttg atgatgaagt tgtatgaacc gctataaaat gagcttgtgg cactcacaag
    20341 gttcataata cctatggcaa acagagctaa tccatttatg atgagatacc agtcgagatg
    20401 gtaaagtttt cgtttatcga ccttgaccac ggaccattgc ctccttaatt tcaggtttta
    20461 cagtcaaaag gctttcgctg attgccttgg ccactggagc agcttcagat gaaccgtggc
    20521 caccatactc gatcagcacc gaaattgcaa ctgcgggatc ctctgatgga gcataagcta
    20581 tgaaccatgc atggtcaccg aggtctcttc ctttaacagg cgttgtctga gccgttcctg
    20641 ttttgccgct catatgaaca ttctcgatgc gggaaccata ggcagtgccg cttttctcat
    20701 ttacaacgcc ctgcatacct tcctttacaa ttgtaaatac ctgtttcttc atttttatgt
    20761 ctgccgtgac cacgggttca aatattttct gggtttttcc atcaggacca atgatcttgc
    20821 tgacgatttg tggtttgaag gttattcctt cattcgccac aaaggatgat aattgaacaa
    20881 gctggacagg ggtgagccat acagcgccct gcccgatggc cacagacaca gtttcccctt
    20941 cataccaggg cacaccctgg gttttccttt tccattccgt tgaaggaagg aggccgcggg
    21001 cttcacttgg aaggtctata ccggatggtt taccaaggcc gacagatgta ccaaagctat
    21061 ggattctgtc taccccaagc cttaatccaa tattgtaaaa aaagacatca catgattcca
    21121 cgattccccg gtgtacattc actgtgccgt gcccaccttt tttccagcat ttgaatattc
    21181 tgttcccaaa tggaaaccca cctctgcatc catagcttgt gttggcgtta attaacccat
    21241 cttccagggc tttgagtgct gttaaaattt tgaatgtgga gcctggagga tatctgccct
    21301 gaatcactct gttttggaga ggatgggtac tatccagcgc aatggcctgc cagtcttttt
    21361 ttgtgatgcc agatgcaaat ttatttggat caaatgccgg ccggcttgcc agggcaatca
    21421 cagcccctgt ttttggctca agcgctatgc atccacctcg tttgccttcc agggcttttt
    21481 cgacaaccat ctgaacgtca agattgatgt tcagatacat attgctacct ggaataggat
    21541 ctttcatttc aagggttctt acctcacggc ccatggcatc cacttctacc cgcttttcac
    21601 catcaattcc tctcagataa tcttcgtaca ttctctccag tccgtatttt cctatatagt
    21661 ctccgggtga ataggttctg tatttctctt ccttgagttc ctcgctgctt atttcagaca
    21721 cataaccgag agtgtgagcc agcaccttgc catagggata atttcttttt ggttcaattt
    21781 gaatgctgac gccagggaga taaaccctgt tggcttcaac ctttgccagt tcgtccatag
    21841 tgacgtcgtc ctttattttg acaggaaaag atgaagggaa attacttgca gcttttaatt
    21901 tctcaataat ttcctcacga tcaatcccca ttaggcctga caaaccatca accgtttggt
    21961 taaaatcctt tatatcctca ggggtaatgt acaggttaaa cgaaggtctt gtatctgcca
    22021 gtatctttcc catcctgtca aacatgatac ctctcggcgc cagtaccttt ttgattctta
    22081 ttcggttcat ctccgactgt tgcctcattt cattgccttg catgatctgt aggtgccaca
    22141 gccgtatgga cagaacaatc atggtcataa tgagaaccca cttacaccat ttgtatttat
    22201 ccggaacggt cttttctttc atgacctttt cgtactctgt gccacggggc tcagcataaa
    22261 acatgtcatt ggtgctatga ttatcttttt tctttttcat tgtggtttat tgaaatattt
    22321 tacgttaaga aaaccgatga gagagaacaa gaataaagaa agaagacctg taaagatggc
    22381 attgggtatc gaatagaaag caacattaaa catgtcccgg ctctctcctc gcgtaagata
    22441 ggaaagggca aggaacaggg aggcttcaac aattactgca ccgctgcata caaaagagaa
    22501 gctgtattta gaatcaatat agatcttgtt tctcataaaa acggaaatga tgaaaatgga
    22561 tgcttttgta aacaaaagcg atccacttgg cgaggtcgat acaatctcct gaattaaccc
    22621 gattaccaca gatgttatga gacctgcctg gggtcctaaa aaaaaggtgg tatagatgac
    22681 aaaaggaata cccacatcag ggttgaaaaa atctattgga agaaagttta caacggcaga
    22741 ctcaaaggtg gttagcacca gcgctatcac gagatataca gcaatctttg tcatttcttc
    22801 accaccagaa cttcttccag tatgttgaag ttgttgaaag gcatcacttc aatttcggaa
    22861 aatattccgg gttttcccct gttgatggtg gttacaatcc cggttgccaa tcctttcgga
    22921 tagataccat ctttgcctga agtaataagc ttgtcgccaa tttcaatttc atcgttcttc
    22981 cgtatgtatt ttaacctgag gagagtttga ccggtgcctt caagtattcc ccttgtgttt
    23041 cgcccctcga cataaacatc aacagaagag tttgtatcgt tgatcaccat aacctttgta
    23101 acccaccggt ctgtttcaac tacctggcca acgaggcctc gtggtgtgat gacaggcatt
    23161 ttaatcgtta tgccactact ttttccttta tccaggataa tgcacttaaa ccagttttta
    23221 atgtcttcgc caataacacg tgcagcaatc atcgttttcg ggcgttgctc catgaggttg
    23281 aggatgctct ttaatctctt gttttcatac tcgagttcag aaattttctg attttccatc
    23341 tggaggctgg cgaccttgtt acgtagttct gtgttttctc tgttggtgcg gataagacct
    23401 atataattct tgaaaatgta gctgacaagc tcgaccggtt tcccaacggt tctcagaagg
    23461 ggaccgctaa cctcaccaaa acctgttttt gcaaccgagt aacccctgag aaagagcggt
    23521 gtgtttgtaa aaataaaaaa agacacaacg aggactgcta tggcaattat tatgacaact
    23581 gtatttttca attcctcgtc aggttaaaaa tacgtagcta tttctttcaa tagtgttatt
    23641 tcatcaaggg ctttacctgt cccttcaaca accgcactca aagggttttc agctataatg
    23701 acaggcaatc ttgtaacctc tttaataaga agatcaaggt ttctgagtaa agcaccgcca
    23761 ccgctaataa cgataccctt atctactatg tctgatgcaa gttcaggagg agtcctctcg
    23821 agagcaatcc tgacagcctc aacgatggcg ttgacaggct cagcgattgc ttccctaatt
    23881 tctttcgatg atatttccag tgtcttaggt ataccaccta caaggtcccg gcctttaacc
    23941 tccatggtga gttcttcacc atcctggctc ggataagctg accctatatt aatcttgatc
    24001 atttctgcgg ttctttcgcc tatgaggaga ttgtattttc gttttacata ctgtatgatg
    24061 gcctcatcaa ttttgtctcc agctaccctc acagaattac agtagacaat accggcaaga
    24121 ctaatgactg ccacctcagt tgtacctcca ccaatgtcaa caatcatgtt tccgttcggc
    24181 tctgttatgg ggagaccaac tcctattgca gcagccatgg gctcttctat aaggaagacc
    24241 tcccttgcac ctgctgactc tgctgactcc ttaaccgccc gtttttctac aggggtaatt
    24301 ccggaaggga ccgagatcac tatccgtggt cgtgcgtaag actttttatt gtggattttt
    24361 tgtataaagt atttaagcat tgcctcagta atttcaaaat ccgcgatgac cccatccttc
    24421 aaaggtcgga tagcgacgat gtttcccggt gttttaccaa gcattttctt ggcttcttca
    24481 ccgacggcta tgactttctt gccgccctta gcgtctttgt gaattgccac gaccgaaggc
    24541 tcattcgaca caatgcctct gcctttaaca taaactaatg tgtttgccgt acctaaatct
    24601 atagcaaggt cttttgatat gaagccaaac aacgactcaa acattctgtt tttcccttca
    24661 ttcttgattt aaagatattg aaaaaaccgc cttaatatac tattttaagg agtcaaaatc
    24721 aacaatcata ggagcacaaa tgctgaaatt tatgcggaag tatgcaaccg gatatctggt
    24781 caaagccatg ttcggactga tcatcatcgt gtttgttttc tggggtgtcg gctccttccg
    24841 ggaacgagac agaattgtgg cggaggtagg gccatacaag gtatctatca tggaatatca
    24901 ggaagagtac aaccggacca tgaatatgta ccgtatgatc tacaaggata ggctggatga
    24961 aaatatgctt cgggagctca aggtgaagga aaaggcaatg aatgatatag ttgacaaata
    25021 tattctcctc atcaaagctg gcgaacttgg tatttctgtc agtaacagtg agttcaggga
    25081 acacctggaa agcatagaaa tgttcaagcg tgatggaaaa ttcagcgaaa tggcatataa
    25141 ggaaatcctt aaacgaaatg gtttggaccc caagcgcttt gaacagtcag aaaagaatgc
    25201 aatcttaaat accaagatga ttaatctcat aagagacaca ggcgccctga tgagtgaagg
    25261 aaacctttgg aattcttatg tgaaggaaaa aggaaggatc aacctgggat atacagaggt
    25321 tgatccggca tcgtatcgaa caaaggttac agttggggat caggaagtgg cagatactta
    25381 tgagaaggaa aaaaatatca gacgtggaga caacatttat aagctgaagt atattctttt
    25441 tgatgaaaaa tcatcggtaa aagatgatgt ggcttatttg gaactattaa aatcaaaaga
    25501 tcttgaggca tatggaaaac agaaaggatt gcaagtagct gatcttggct cgatgaaaga
    25561 aggggatctt cttaaaaggc tgaagaatct taaggctgat gaatggctaa gagggatgaa
    25621 aaaaggtgag atctcacttc ctgtccgggc tgatgcaaaa tcttacatct ttcaggttgt
    25681 ggatatcgaa gaaggaaagc ctattgatag gactttggca atgaaagaga taaaagaccg
    25741 gcttatcaat gaaaaggcga aaaactttgc aaagaacgtg gcccaggaat taataaacaa
    25801 gaagaccgtc gattcaaaaa aggataccgg ttttgtaccg agaaccacct tggccattcc
    25861 gaaaatcgga cagattccga aggatgacct cggcgtttta tccctttcaa aggaattccc
    25921 catctacaac aaaccagtag agatcgatgg gattttttac gtatttcact ttaaagagga
    25981 gaaacttcca gacaaagacc agtgggaaaa ggacaaagaa agttacaagc gatatgcact
    26041 ctctaagagc cagagtgatt ttcttaagtc ctttatggaa ggactccgca aaaaagagaa
    26101 ggtgaagatt gagtggaaag agataagctg aggcatgatc atccttggca tagatacgtc
    26161 gtgcgacgat acgtcctttg ctgttttaag gaatggaaga gagcttcttg ctaacctcgt
    26221 ttcaagccag atagacttac atagtgtgtt cggcggggtt gttcctgaaa ttgcgtccag
    26281 aaaacatgtt gagcttgttg accagttata ccgcgaggtc ttgcagaatg cgaatctttc
    26341 cggtaagtcg attcaggcaa ttggtgtaac atctggtccg gggcttattg gctcagtcct
    26401 catggggcta tgctttgcaa agggactttc cctttctctc ggtataccca ttatcggagt
    26461 taatcatatt gaagcccatg ccatgagtat cctcctggag tacgatgtgg aattcccctt
    26521 cattgccctt gttgtatcgg gtggccatac cataatcctc cgaatcgacg attttggatt
    26581 atataaggtt cttggtagta cccgggatga tgcagcagga gaggcattcg acaagattgc
    26641 aaagtttctc ggattaggct atccaggtgg tcggattatt gagaaaatgt caaaggaggg
    26701 gaaacctgat catgtttttt ttccgagacc tatgattgat gataacaatt atgatttcag
    26761 tttcagtggt ttaaaaacat ccatgataaa ttacattaaa aaatataatg taaataatga
    26821 aaatataagt gatatattat catctttcca ggaagctgcc tttgatgtcc ttgcaaacaa
    26881 aacattgagg gcggccaaga acatcggaat tccaagaatt gtagttggtg gtggcgttgc
    26941 ttcaaacgga aggctgagag ctatttttca cgaacggtgc tcaaaggata gaattgatgt
    27001 gtttttctcc tcacctgagt tttgtacgga caatggggct atggtagcca aggtagccca
    27061 ccattactac gtgacaaata agacatctcc cattgatatt gcggggtact cgcgaatgaa
    27121 gttccattga tccatgctga aaaaaaggct ttcgcaacat ctcattaaag atagaaacat
    27181 aacagataaa atggtaaggc tcgcacaatt aaaggaagac gacattgttg ttgagattgg
    27241 ggcaggtcat ggagatctca ccaaaagcat cgctgaaaaa gccggccatg tgtatgcagt
    27301 tgaattcgat aaagatatga ttgcccgtct taaaacgctt gcaaagaagt acggtaacat
    27361 aacggtcatc caggaagatt ttcttaaaac atcattatta cccttcagtg cagacaagaa
    27421 cataataata atgggtaaca taccttacaa aattactggc cctataatct tcaagcttct
    27481 tgaagataga aaaatagtct ctgccgcata tctcaccatg caagccgagg tggctggaag
    27541 gatcgtaagc aggtcgcaca agcgtaccta tggagcaatt tcagtggtat gccagatact
    27601 gtgtgacgtg aagattctat ttaacctgca agcctcagtc tttgtgccac ctccaaaggt
    27661 cgatagcgcc ttcttacgga tggtaccaaa agaggaaaaa aagaacattg accgggaact
    27721 gctgggtttt gtgagggcat ctttccaaaa taaaagaaag tatttaaaat atgccctttc
    27781 aaagacatac acggaagata tgattgagcg catctatagt ttcatggggc tttcagacac
    27841 tgctcgtgct gaagaaatta gccctgatca gtttgaaaaa atgtataatt ttgtgaaagg
    27901 taatggttga ggtgagtata ctacaaaagg gtgctgtgat tggatttaca acaacgatac
    27961 cggtggaggt aatctttgcc ggtgggtata cgccatgcga tcttaacaac atctttgtta
    28021 ctgacagtaa tcccatgcaa tatattgaaa aagccgaaag agatggattc cccaaaagca
    28081 tgtgcaactg gataaagggt atctatggtg ttgttatgaa gcatcccgtt gatgcagtaa
    28141 taaccgtgat gcagggagac tgcagcaata cccaggcttt aactgaaata ctgcgttaca
    28201 aaggcattcg aacaataccg ttttcattcc cctttgatcg tgacaggcag gtgttgcaga
    28261 gagagattga aaagctaaag aaagcgctct ctgtaaaaga tgagcagctg aaagcagttg
    28321 aagaagggat gagtaaagct cgttcagccc ttggagttat tgataggatg acatgggaaa
    28381 aggggcttgt ttccggctac gacaaccacc tgtggcttgt acgagcgtcc gatatgctcg
    28441 gagattacaa tcggtacaat tctatggccc gtgattttat tgaagaggta aaatcgaata
    28501 aacccaaaga aggtgtgaga attgggtgta tgggggtgcc acccatcatc ccagatctcc
    28561 atgatttcat cgaaagtgtt ggcggccacg ttgtctacaa cgagacccaa agacaattta
    28621 tccttccatt taaagtaatg aatcttacag acaggtatct caaatataca tacccctatg
    28681 gcattttttc ccgactggaa gacattcaaa aagagattga cagaagagaa atacagggca
    28741 ttattcacta tgtccaagct ttctgctacc ggacagttga agacgttatt ctccgtgaaa
    28801 cactgggcat acccatgatt accatagaag gcgacctgcc taaacccctg gattcgagga
    28861 caaagctgag gatagaggcg tttatagagc tcctgagcaa cgctggtgat gctgcatgaa
    28921 atcctttgtt caccagacgc cacaagagcc ggtagagaaa acacgaaaac tcataattga
    28981 gagaaacaaa attggatttt ttaaagcgat tcttgaatcg tacgaggatg tggcaatctt
    29041 cactgtttta gatggaaaat cagggcttat cgagttgatc tattccacct gttttgaaga
    29101 agagatttca gcgattgttc aggatatgat acattgtggc attgctatca aggaggccac
    29161 tgatggtgaa taaggaaaac atattaagaa aattattgga cgggaatggg ctctatggag
    29221 acatattctc agagaaaaga acttatacac atattcaact ggaatccgat aggatcgaaa
    29281 agcttgagcg gggtcttgat gaaggggtag gcctacgtct cataaatccc tggaaaatgt
    29341 attttgcatc aacaaataat atggatgaag ggaacttgct cgacatggct ggtactttgt
    29401 ccaggttttc acgtgaaaag tcaaaaagtc aacaaaaaaa aatacaaaaa caaaatggtt
    29461 tctatccatt tacaatcatt aaagatcctg aaggaataga catcgatgag aaattaacaa
    29521 tggtgaaaaa aatcgggact atggcaaaaa aaatggagcc aaggatacgg caggtcaggg
    29581 tcgtttacag ggatacccac cagacaacat atatatatac gagtgacgga aaagatttca
    29641 aagataaccg gacccaggta gtcctgagcc ttcttctcgt tggggaggaa aaagaggaaa
    29701 tacagacttc atatgaggct ataggtggtt tttacggcta cgaatttttc accgatgaag
    29761 tggtagagca gttcgttcta aagacagtta agcgcttgtc cggtttgctg tcagcgaaag
    29821 aagcacctat gggaacaaaa acagttgtgc tgtcatctga ggccggaggt actatgattc
    29881 atgaggctat tggtcatggc ctcgaggcag acctggcaat ggaaggactt tcgtgttaca
    29941 aaggcatgct tggggactca atagcctcgc ctttaataaa tgttgttgac gatgcaacag
    30001 tacctaatct caggggaaca tatgccttcg atgatgaagg taccgagtcc cagcgcacag
    30061 ttttagttga gaatggggtc ctgaaaaact tcctcttcga caagttttat gcaatgaaat
    30121 atggaaagaa ttccaccggg aatggaagaa gggagtcgta tcgttataaa cctattccga
    30181 gaatgagcaa taccatgata ttgccaggca gtgaagatcc ggcagcgatt gtctcaagtg
    30241 tcgatgacgg agtgctcgtc gtgaaaatgg ggggaggaca ggtagatact gtccgtggtg
    30301 attttgtctt cgaaatctct gaaggttaca taattgaaaa aggacgtgtt ggagaaatga
    30361 ttaagaatgc caccataatg ggcaacggac tcaaagttct tcacgagatc gacatggtgg
    30421 gcaatgatct cggttacggg ataggaacat gtggcaaaga tggacagggt gttcccgttt
    30481 ctgatgccca gcccacactt aggataccag agattgtggt tggtggtaag tctgcaggat
    30541 agggatattg cgaatgaaaa acacatattt cttaaagata aaacactgcg aatgtcattg
    30601 acattgtgaa ataatatacg ttatatagac aaatagatac cattaataac taattcaccg
    30661 ttaatggtca tctgaaacag ggtcagatga ctgctcaaaa gaagaggtag atatgaagag
    30721 gaccatcgga ttttactttg ctatgctttt ctttgttatg tttataacaa cacctctctt
    30781 ttcagccact gaagatgatc ttgtcgggac ttggagcatg taccattcag ccaaactgaa
    30841 ggtcagccgt attggcacga caaccagtca aaacaattca atagtaaccc tgaaaggtga
    30901 tggtaccttt accatggtgg agaacgatcc accggacacc tatacgtata cagggaattg
    30961 gcaactcatc aagaaaggta agaaggtgct catcagcttt gaccagggcg ggcgggatga
    31021 gttgattcgc cttctcgaag actggatgat ggaggtggcc agtggatatg gagtccctat
    31081 ctccgatatc aatatcagcc tagcaaaact gaccatatcc cagccaagta taccgaaaaa
    31141 gacgaacatc cccaaaacga ctacaataaa ggcaactgga ttggtgagtg cttacttaga
    31201 cggtatgttc ataacaagga aattttcata tgacagcaaa gtctctttca tctgtagaca
    31261 ataaagaaca gctggacaat atgatagcat aacagccttc atatttattg aaactgataa
    31321 aacggattta tgacaaaaga ggttgactgg ctagaattat ggcgtgagct ggtatccaga
    31381 aaactccatc ctgagacgga aagcctggtt caaaaataca gggctcatgg tcaaaagcat
    31441 aatgaaaggc cagacagcct tctcgatttt gtactgaaaa agattgaccc tgaagacacc
    31501 gcggttgata tcggagctgg taccgggcgt tggacaatcc ccctggccag aaaaaccaga
    31561 aatgtgactg caattgaacc agtttccggc atggtggcaa tgctaaaaga gaatcttgat
    31621 tatgccaaca taaaaaatgt aaagattctc ttacaaacat gggaggacgc agctctacca
    31681 gtccacgata cagcagtttg tgcccatggc atgtatggta gtcctgattt ggctgaattt
    31741 gtgcgtaaga tggaacgtct tgcccgtaag ggttgttacc tggccatgag gctgccaccg
    31801 gcagacggca tcctatcaga actctccctg cagatttatg gatgccggca cgatagccct
    31861 gatgctatga ttgcctataa tgccctctac accatgggaa ttgatgtaaa tattttggtt
    31921 gaaaccagta tagtaaactg gtttaatcaa acaatggaag atgcctttat ccgtgcaaag
    31981 agacacctgc gtatccaaat gtctgatacc acctacgatg atctgattca ttccacatta
    32041 aagcgccggc tgaatttctc tgatggagca tatatatggc ctgacggcat gcgttcagca
    32101 cttctctggt ggacccctgt tcactgaaac atttatgcct atggttatga ctgatgcaaa
    32161 taggaggttg tacatgatga aacctgcatc tgaggctggt gacatcctca aggagaccat
    32221 ggaggctatc caatccagcc ttggtaatga aatggaaaca attactgttg agaaaacagt
    32281 cattggtctg tttttcactg gagtcaaact ggacacaggt gagggagggc tgtgttttac
    32341 cccagttaag tctattcctg aagccgtgtg ttgtccaagt tctgcacagt ttatgcctgc
    32401 atcgggaaaa cttgaaggca gaaaagcaac acgattttta aatgacatgg cttcaggggg
    32461 tcctttaaaa aaagctgttg cgattgccgt aatgaatgcc ctttcttcga tatgctggaa
    32521 aaggcaaaac ccggaaacat acagaatcag gattggtgtg gatcctattg atgaaatggt
    32581 cattcctgat gatggctttg tcgttgtggt tggcgccctt gttacggctt tgaaaaaact
    32641 gaagcagaga ggcaaaccct ttgcagtact tgagcttgac ccttcaatac tcaaaggtga
    32701 cgaactggaa tattttgtgc cctttgcaga ggcctataag gttgtcccta aagccgacat
    32761 tcttgtcatc acaggaacaa cgctgattaa caacaccctc gaaactcttc tggagaagaa
    32821 gaagccggga gccgaa
//
LOCUS       DAOOMC010000015        32615 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdus sp. isolate AMR_MDS_2685
            NODE_1144_length_32615_cov_6.493090, whole genome shotgun sequence.
ACCESSION   DAOOMC010000015 DAOOMC010000000
VERSION     DAOOMC010000015.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864760
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdus sp. (wastewater metagenome)
  ORGANISM  Syntrophorhabdus sp.
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae; Syntrophorhabdus.
REFERENCE   1  (bases 1 to 32615)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 32615)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,729
            Genes (coding)                    :: 2,708
            CDSs (with protein)               :: 2,708
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 7 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32615
                     /organism="Syntrophorhabdus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1144_length_32615_cov_6.493090"
                     /isolate="AMR_MDS_2685"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2699756"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..395)
                     /locus_tag="PK125_02640"
     CDS             complement(<1..395)
                     /locus_tag="PK125_02640"
                     /inference="COORDINATES: protein motif:HMM:NF018513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HPB37040.1"
                     /translation="MSEIGIGVIFLILLLCLFLTGLELAFAMGIIGVLGFAWIVNWKA
                     AMGLMANDVFDALANYGLTVIPLFVLMGQIAFNAGIAKRLFDCAHKFLGHIPGGLAIA
                     TVAGATAFKAICGSVAATSATFASVAVPE"
     gene            complement(388..873)
                     /locus_tag="PK125_02645"
     CDS             complement(388..873)
                     /locus_tag="PK125_02645"
                     /inference="COORDINATES: protein motif:HMM:NF016200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HPB37041.1"
                     /translation="MEGLHAVILRISKVMDVVGGVVLSLMMLITVTDVILRFFGKPIT
                     GTYELVFLGGAIVIGCAIPQTSWQGGHVNVDLVLDNLPGMLKKIFMVFTRLLGIIFFA
                     FLSWNLLKYGAMLFDKGEVSLTLHVPYYPVAYILSLCAFVECLVLLSGLIKTICEVNH
                     E"
     gene            complement(903..1931)
                     /locus_tag="PK125_02650"
     CDS             complement(903..1931)
                     /locus_tag="PK125_02650"
                     /inference="COORDINATES: protein motif:HMM:NF037995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HPB37042.1"
                     /translation="MKRLSVAKILLLALFLSALVIPIQVQAQDKVITLKVANWFPIGH
                     KQTPLLESWCKEVEQKTKGRVKVNYYPGGTLVPAVQAYDATTKGISDVGNHVLGYTVG
                     RFPLTSVLDLPLGYPNNTVPTKLANAFYAKFKPKEFNDVKILWFHAQSPGIIHTKSKP
                     INKIEDLKGMKMRTYGPNARLMSLLGGTPVAMPMTDVYDALSRGVADGLMCAYEATEG
                     FRFGDQLKYSTENFGTSYSAVFVIVMNKDKWNSLPPDIQKVIDDLSPQYIEKYAAMWD
                     DIEKSGKAYLVKRGNKIITLSKEEEAKWVAKAQPIFDEYVKSMKEKGLPGEEALKFAM
                     DYLKPYRK"
     gene            complement(2270..3613)
                     /locus_tag="PK125_02655"
     CDS             complement(2270..3613)
                     /locus_tag="PK125_02655"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HPB37043.1"
                     /translation="MIRVLIVDDEYQLLEAFKKKLTREGMEVFTASNGKEALSIVRQE
                     TFDIGLFDIRLPDIDGVQLLKELKELQPTAEVIMLTGYASIDTAIRSMKLGAYDYLTK
                     PVKLSELHAVILKAFEKKELKEKAIVLEEQLQRIEAHDRFIGESKEINAVKKAIALVG
                     KSNIPVLILGETGTGKELVARAIHGLSTRSHNPFVIINASNLQESILESELFGYKKGA
                     FTGAHIDKMGLLQIANTGTFLVDEVADMGTSIQSKLLRVLETGVFRKLGDTKEITVDV
                     RFIFATNKSIEEEIKANRFRKDLFYRLNTFVIQVPPLRERKDDIPILTEYFLEKHARK
                     GIKKVVSKRAMNLLMDYHWPGNVRELANIIERAVLISTDRYEIVHEDLPQTIFIPSFV
                     EVDKKSSLSEDALRLDIMEKEHIKKVLEISGGNKSKAARLLGISRKKLYQKMDNH"
     gene            complement(3623..5131)
                     /locus_tag="PK125_02660"
     CDS             complement(3623..5131)
                     /locus_tag="PK125_02660"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPB37044.1"
                     /translation="MKNGPRVEFNAIISILDSLPMGVFVLDQKRKIQWMNKWVLHHVQ
                     GDTAHHSIEKYCYTKIFNLKSPCKNCPSLRTLTTGRTEHAEIKSEYKGETKCYLVTAT
                     PLQKEYRDEQILIIETIQDITYQKKAEEDLRHLNDFNAAIIENAPVAIFTIDRNGKFI
                     SVNPALATLSGLGAEAEEKLVGFNWLENPYTVRCGLAEYIKKGLEGEPFELQDFPFTT
                     YRGDQGQYIHFRGVPLRGKNGNVEGLLCIIEDNTEKVRAKIQLIQDAKMSVIGRLMTG
                     VAHELNNPLATIAANSELACDLFQGLANEECKDGALDELREHLDVIQEQAFRCKNIIK
                     DMIDLTRKKGFEVKEIDLSLCLEEVFKVINFKKLNIRLVKEIESNLPHIRGDYNAMKQ
                     CFMNILQNAIDAIEIRAGGVIKVRALPFHDSVYIEFEDNGEGIDEGLKDKIFEPFFST
                     KKTGKGVGLGLTLCYEFINKMGGSIEVDSTLGRGTTFKVTMPVYRDDLQSMG"
     gene            5321..5393
                     /locus_tag="PK125_02665"
     tRNA            5321..5393
                     /locus_tag="PK125_02665"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5354..5356,aa:Ile,seq:cat)
     gene            5498..6211
                     /locus_tag="PK125_02670"
     CDS             5498..6211
                     /locus_tag="PK125_02670"
                     /inference="COORDINATES: protein motif:HMM:NF014630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc ribbon domain-containing protein"
                     /protein_id="HPB37045.1"
                     /translation="MEQELKTIYETQIIDIQIMENEKKLVSTPKKIEKMDSDIKAMQD
                     KISKEKEILEELEKERKRKEKDLELDKEKVKKFESKLYEVKTNKEYQALLKEIETAKL
                     MNDKEEEEIIEIMVKIEDLKKDYESTSKLLKEKEKVTEVEKKKLLAEIDSVDKTINEL
                     KQQRDNLLSVVDKTLRETYNMLISKRGGMAVVNVKNGVCLGCFMNIPPQLFIEVTKNN
                     RLILCPSCNRIFYFTEASQ"
     gene            6219..6620
                     /locus_tag="PK125_02675"
     CDS             6219..6620
                     /locus_tag="PK125_02675"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: protein motif:HMM:NF012304.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HPB37046.1"
                     /translation="MEWHIYIDGASSCNPGHAGAGLVVFDEFGNEIGRDSAYLGEMTN
                     NMAEYEALVRALSRAFEANIKSVSIYTDSLLVANQILGKYKIKNTTLQKYAEIAKNLI
                     HTFDHFAVQYIPREKNKIADKLAKEAIKRKG"
     gene            6613..7077
                     /gene="rnpB"
                     /locus_tag="PK125_02680"
     ncRNA           6613..7077
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="PK125_02680"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            7223..8776
                     /locus_tag="PK125_02685"
     CDS             7223..8776
                     /locus_tag="PK125_02685"
                     /inference="COORDINATES: protein motif:HMM:NF014425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HPB37047.1"
                     /translation="MTRPLLAICVICSILFFLNLQSRDFWAPDEGDFALIVRELPDNH
                     IVPHLNGKPYGEKPPLYYYIIYLSKAIFSWFRDEASLRLPSGFFAIIGALALFLTLYR
                     FINNHQAILATCILISTPLYYWQARYLQADMVFAVFVSFSLLLFFWFYKTRKEYLIYL
                     FFLSTSLAFLVKGPLATALIIPVVILFLFFEKSFRIIKIRQLAIGVLICLAVILPWYM
                     AVYLKEGAPYLYENVIRQNFIRFFDAWSHKRPFYYYFKTLPLDVFPWSLFLPLGLVQA
                     FKRFRSNREMNFFLVWFVWMFFFLSLSSGKISKYMLPALPAIATIISLSFKIEENKYN
                     KIVFACIIFLLFTMGMGLFFFKQDLYPEFLPERIIFGSLCCAFGMGLLLLRTRRIIYS
                     FIAIFSFMIFSYTIGNAGNIFEKWNMHKSPRFISEQMKPYVQDCTPWVYYGSMRGVYV
                     YYVQKKAIHVEEHDITGLKLVAQQISSFYILTKKRDLAEVHKALDKVDIVFEEKNHDS
                     PMIFARYAR"
     gene            8900..9352
                     /locus_tag="PK125_02690"
     CDS             8900..9352
                     /locus_tag="PK125_02690"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HPB37048.1"
                     /translation="MSNFFDKLTVRHLTKDDLDSIVEIDTKVLGEARREYWVTKIIKE
                     ADTRPSDASLVSEIDGKVVGFILGEVSGWEFKVPNNIGWIDTIGIDPDYQNRGIAKVL
                     ATALVANLKTYGVDTIYTLVNWNDWDLLQFFHAMGFSRGDMINLVLKV"
     gene            complement(9468..11204)
                     /locus_tag="PK125_02695"
     CDS             complement(9468..11204)
                     /locus_tag="PK125_02695"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HPB37049.1"
                     /translation="MGRFVVLFFVIVILFGCSTAQQTTPVNYQQSPPTNHQQGRPVSQ
                     KTSEPQVVAKVEPIKKSEFVLKNDVGSVKVEYLTQDDEDDITILLEPEQFQDFDIPIV
                     FNDAVKYYIQWFSTEKRKVFGNWLKRSRHYVPIIREILKDQGMPEDLVYLAMIESGFN
                     PKAYSHAKACGPWQFIYATGGRYGLKVNYWIDERRDPEKSTVAAAKYLRDLFNQFGCW
                     YLAAAGYNAGEKRVERAIEKHNTNDFWELSNYNALPRETRAYIPQLIAAAIIAKDPEK
                     YGFGSITYDAPIRFELVRVPRGTPLSVVAKSASIDLADVRSINPEILRGITPPNMDDY
                     KIKLPQGTNIQAFTSRLQVAMSHERQIRDVISYKVKKKETLASIARKYRLNQDDLYLV
                     NECEGELRIKPGMVINLPRFSGPSKRKTELAKNAIESHEPKPVKGESLAKSGKVRVAQ
                     SGPRQQTQGPVIHVVRKGETLASISNKYNIDIAQLKAANNLKGNTVSPNMKLRLARYS
                     QKKSAQKESIGRESVKKKAPVRYHVVKKGETLKSISSKYNIGVADIKLSNNLKGDKVV
                     PKQKLKIIVSEG"
     gene            complement(11298..12239)
                     /gene="fmt"
                     /locus_tag="PK125_02700"
     CDS             complement(11298..12239)
                     /gene="fmt"
                     /locus_tag="PK125_02700"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HPB37050.1"
                     /translation="MKVVFFGSSSFSVPPLQSIASFVSCVVTRRSKPKGRGYVLEDTE
                     VRREAMNLQLPVIEIDSFKDEAIKDIERLRPDLFVVASFGLIIPKWALDIPSIGPINI
                     HPSLLPRYRGPSPIQWTILSGDSETGVTIIRMNEKMDAGNILYQETIAVGPEDNAITL
                     SDRLSKRSAVILPDFLSETALVGLHEGLTQEHEKATFTPILTKEMGRIDWSKSGTEIL
                     RHVRAFIAWPTAYTFLDGLLLKVLEGSVAVGEMGQVERHTIPGTIFAISKEGIHVCTG
                     SDGFIIRELQLQNRKRMKAYDFSRGYRGLEGKVLKYD"
     gene            complement(12236..12733)
                     /gene="def"
                     /locus_tag="PK125_02705"
     CDS             complement(12236..12733)
                     /gene="def"
                     /locus_tag="PK125_02705"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: protein motif:HMM:TIGR00079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HPB37051.1"
                     /translation="MAYIQIKTYPDPILRKACEPIESITGETVILCNNMVETMRLAQG
                     AGLAANQVGVSSRVAVLDPRTKKESKPIILINPVIVEQDSEEISEEGCLSLPRFYEYI
                     KRAKKVLVRGINIDGEPFEMECEGFLARAIQHELDHLNGLLLIDRLSPVKKDLFKKKY
                     MKDRK"
     gene            complement(12817..13695)
                     /locus_tag="PK125_02710"
     CDS             complement(12817..13695)
                     /locus_tag="PK125_02710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB37052.1"
                     /translation="MKKNIIVRLWVCSFIGIAFFCAQGFSCAGAADFADILTEHFQKK
                     VPVAEFDPQMTMDQAMKTQEKYVANLSKDYGEAVGYKAGLTNPNVQKAFGVTNPVRGT
                     LLSKMMLKSGAELPAAFGAVSMIEGDLCVRVGSNDINQAKTAEEALKSLDAVIPAIEA
                     ADMVFAKGVKFTGPAIAAINVGARYFIMGNPIQLSPTRDWQERLENFSMTLKDETGAD
                     LAEGKGSAILGHPLNVVLWIKDSLVAEGKALKKGDILSLGSVTKMIPAKAGSTIKGSY
                     MGLSPEGPVEISVSFK"
     gene            13927..14646
                     /locus_tag="PK125_02715"
     CDS             13927..14646
                     /locus_tag="PK125_02715"
                     /inference="COORDINATES: protein motif:HMM:NF014017.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HPB37053.1"
                     /translation="MKYHIGTSGWHYKHWKNIFYPQYLKHSDWLSFYAQHFDTLEVNF
                     TFYRDVKPSTCKKWYSTVPENFLFSVKISKYITHIKRLKVDTHSIERFIESVRCLGEK
                     TGVVLVQLPPELAFDEPLMRYFFALLDRKLRYTVEARNKSFIDDRFFELLKENNIAWC
                     IADSAGRFPYHEAVTAPFIYIRLHGSQRLYASDYSDEELKVWKDKILNWNRDIFVYFD
                     NDFYGYAVRNALKLKSLLISC"
     gene            15391..16149
                     /locus_tag="PK125_02720"
     CDS             15391..16149
                     /locus_tag="PK125_02720"
                     /inference="COORDINATES: protein motif:HMM:NF024798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase"
                     /protein_id="HPB37054.1"
                     /translation="MKQVFRAGGFLTEEIDSLLQDQRLALYPEILNQKTKGINYLHPR
                     FGLLSRSSIKRGQQVLKENRITLKNIEASYGIPREVLVAIFRLETNLGSNLGQYRVFN
                     SLLTLTVIVNRRSVWAAQELFSFLTLCNSNRINPFSIKGSSAGAFGLCQFVPSSYLAY
                     GIDGNGDGVVDLFDFSDAMASTANFLKSHGWHSRNAKRQFRAILAYNHCENYAKAVLT
                     YSKQVRLSHNKKPNTTKINNRHQNRQKGKDFVRI"
     gene            16645..16902
                     /locus_tag="PK125_02725"
     CDS             16645..16902
                     /locus_tag="PK125_02725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB37055.1"
                     /translation="MSFRHLVGPATCTGLDIESLKSLITVGSLETLYRLEGQSLSDIM
                     TMHIEGGFAGTCFSRREAPPVSEMSVAEKVLADYTFQSFSA"
     gene            complement(17568..17843)
                     /locus_tag="PK125_02730"
     CDS             complement(17568..17843)
                     /locus_tag="PK125_02730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB37056.1"
                     /translation="MGSGTAGCGGGAGALGGALGAGALGGALGAGALGGGLGGVSGTL
                     GCGLGVGSGTFGAGLLQPTSNIKARAIIMAVFIGFVPPINNLDPYSS"
     gene            17959..>18463
                     /locus_tag="PK125_02735"
     CDS             17959..>18463
                     /locus_tag="PK125_02735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB37057.1"
                     /translation="MPDTPNVYVAPGIAVDLFFWNGWWWRPWRGGWYRSRYYDRGWGY
                     YSSVPSFYYDVDPGWRGYYRNRSWRGHRWNYEPIRYRYLRENWKRWHDNRYWERQRTW
                     GVQGYRPPPPAKRQELRQQRERDYFQRREVQQHRQQMQQKRIPQGKVQQPPPQGKIER
                     PGHQGKIA"
     assembly_gap    18464..18563
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<18564..>18779)
                     /locus_tag="PK125_02740"
     CDS             complement(<18564..>18779)
                     /locus_tag="PK125_02740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB37058.1"
                     /translation="AQTEKPFPVGLGALSSPEVVAAVLYPEEEVQLEQSFPDDLGALS
                     SPEVVVLMVESSPEVVAAVLYPEEEVQL"
     assembly_gap    18780..18879
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <18880..19073
                     /locus_tag="PK125_02745"
     CDS             <18880..19073
                     /locus_tag="PK125_02745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB37059.1"
                     /translation="GQGRSPEGRQRMQRKVEQPVQPQGKIERPGPRGRVSPSEPPGQG
                     RSPEGRQRMQRKVEQPVQP"
     gene            19730..21406
                     /locus_tag="PK125_02750"
     CDS             19730..21406
                     /locus_tag="PK125_02750"
                     /inference="COORDINATES: protein motif:HMM:NF012570.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dynamin family protein"
                     /protein_id="HPB37060.1"
                     /translation="MTQRILSPELRKQYESVRDLLEACLGLTEKCSDMEAYRIVQDRL
                     SHLQSAALFVIVGEVKSGKSSFVNALLGEDICDVAPDPCTAVIQELVYGEVREKKSLG
                     DNWERVSLPKNILQEISIVDTPGTNSIIRDHQSIIESYIPQSDLVIFVFPAKNPHTAS
                     AWDFLSLIRQDWHRKIVFVLQQADLATQHELTINQERVKQYARARNVQNPVVFTVSAK
                     REFEGATDSGYAEFRNFLTRAIQSGEVWNMKIEGSRDTAKKIVNGLIARLRSEQQAIA
                     DDMTFYKNLLSKVEARREKANALRRLAVDSLCISYDRLASKLEEDFTEGLGIGTVLLR
                     AIPILRDKDVKTWLKELQANFESTSKQVIDTESMRVSKDISDEIMLMFSELTDAIVHR
                     QSTTQEVFSTKDHDRTEILTHLQKQLQELRIADIVTNKGIQGSDIGKLSLAGGGIAAL
                     GAVIAFATQLVVLDITGGIIALVGAGLIAVTLIWKRSSILHDFSQKMNKSRSEFRERL
                     DKEIARIFEKLFMEIEHRLEEPLSRLNSKAGQLSILVAEGERVNELSKSM"
     gene            complement(22008..23237)
                     /locus_tag="PK125_02755"
     CDS             complement(22008..23237)
                     /locus_tag="PK125_02755"
                     /inference="COORDINATES: protein motif:HMM:NF018485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPB37061.1"
                     /translation="MKRHYAWVIAWTGALVVLLAHGFGRMSYSVILPPMKDGLALTYT
                     QVGLIGTGNFIGYLCLAVIGGFLAARFGARNIIFVSLLIMGVCLFLTGLSGSFAFAFA
                     MRFITGMGNGGSYIPVMALPAAWFVVRKRGLGTGIATVGTGMGLSLAGIILPYFIVNY
                     GHEGWRYAWYAMGIIVFVGSFVVYALERNTPAEKGLSMYGSAGDEKNSQMDRQGGVSI
                     LSAWHDVMKEREVWKLGGVYLMYGFSYIIYLTFFIAYLTREIGLSEKTAGWMFATLGL
                     CSISSGVLWGSVSDFFGRRFGSMYAYVVLALSYLMFAFWTDLPGLYISCIIYGFSISS
                     IPVILAAAAGDAVGPKLAPAGLGCITLFFGIGQAFGPAVAGYIKDVTGSFTGAFILSS
                     AVSLAGALASLFLKKRA"
     gene            complement(23421..23945)
                     /locus_tag="PK125_02760"
     CDS             complement(23421..23945)
                     /locus_tag="PK125_02760"
                     /inference="COORDINATES: protein motif:HMM:NF014912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin family protein"
                     /protein_id="HPB37062.1"
                     /translation="MDTKERLNALEVALNNEMKEREFYLKNAERTKNALGKAMFKQIA
                     DDELEHYERLKQLKIEWEKKGIWPETLPLIVKETDIKEILTKFLQKVDQTAASDDDDL
                     QAIRTAIDFEQKGAEFYRRLRDAVTDKKEKDFFDLLATIEYEHYLSLKDVEEYFLSPA
                     DWYRAHEHHSLDGA"
     gene            complement(23992..24327)
                     /locus_tag="PK125_02765"
     CDS             complement(23992..24327)
                     /locus_tag="PK125_02765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB37063.1"
                     /translation="MMWTRSGEDITRSATGMVTTGTMREFPTSDYNDSGKTGVTISTG
                     KESIPGVSGSTAPSRQTRGRSSDVKESGNTVQDQRFASINSEGDNPRYSIRGKDLDLR
                     EYLKAEDSV"
     gene            complement(25121..25804)
                     /gene="aqpZ"
                     /locus_tag="PK125_02770"
                     /pseudo
     CDS             complement(25121..25804)
                     /gene="aqpZ"
                     /locus_tag="PK125_02770"
                     /inference="COORDINATES: protein motif:HMM:NF012455.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0015250 - water channel activity [Evidence
                     IEA];
                     GO_process: GO:0006833 - water transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin Z"
     gene            complement(25854..26300)
                     /gene="mscL"
                     /locus_tag="PK125_02775"
     CDS             complement(25854..26300)
                     /gene="mscL"
                     /locus_tag="PK125_02775"
                     /inference="COORDINATES: protein motif:HMM:NF013869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large-conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HPB37064.1"
                     /translation="MFKEFKEFAMKGNVLDMAVGIIIGAAFGTIIKSLVDDVLMPPIG
                     LLLGNVDFSNLFLTIKEGKVAAPYASLAAAKAAGAVTVNIGVFINTIISFLIVAFSVF
                     LVIKNVNRFRKAPPPPDPTTKDCPFCFSVIPIKATRCPNCTSEIKT"
     gene            complement(26483..26746)
                     /locus_tag="PK125_02780"
     CDS             complement(26483..26746)
                     /locus_tag="PK125_02780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB37065.1"
                     /translation="MRHGFVVILALTLVLGLSLGGCGCFQEAKKGETPPPAPAKVVPP
                     APACPPCPACPPAVVCPPEKTCPPCPACPPCPAPAKPAKKDRN"
     gene            complement(27431..27640)
                     /locus_tag="PK125_02785"
     CDS             complement(27431..27640)
                     /locus_tag="PK125_02785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_177722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dodecin family protein"
                     /protein_id="HPB37066.1"
                     /translation="MAESVYVVNEIVGTSTKSWEDAAKLAIETAAKSVKDLRIGEVVK
                     QDVTVENGKVTSYRVRLNVSFKYHK"
     gene            complement(27790..28095)
                     /locus_tag="PK125_02790"
     CDS             complement(27790..28095)
                     /locus_tag="PK125_02790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB37067.1"
                     /translation="MNISDWLDEKVAEGVDVSHIRLPDELAYNEDPEETIFFKEINPC
                     GMFCTGNHPFSTVEKFGHWYYSRGQDKRAGIHSNEMEWTLFTKDKDLAIQVAKSHIE"
     gene            complement(28110..28400)
                     /locus_tag="PK125_02795"
     CDS             complement(28110..28400)
                     /locus_tag="PK125_02795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB37068.1"
                     /translation="MIEINGKEKDMLVKIENNQFTGGAYRRATFIDKVCRNKSDRLIL
                     EGLCKKGLVEKGLGGTVAGDVYDGCWLTQKGKEALREGQVKDAIQEDSSNSV"
     gene            28612..29328
                     /locus_tag="PK125_02800"
     CDS             28612..29328
                     /locus_tag="PK125_02800"
                     /inference="COORDINATES: protein motif:HMM:NF020771.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CerR family C-terminal domain-containing
                     protein"
                     /protein_id="HPB37069.1"
                     /translation="MKTLRKDTARTQKNLLIAASKIFAEKGFRDTTVAEISKRAKTNL
                     AAINYHFGDKETLYREAWRHSFRESIKIYPPDGGVDEKASPEHRLTGRVMALLRRITD
                     DGNAEFSIAHQELAKPTGLLEEVMHQEFEPLQQRFESLVHEILGPHSSKIQVRFCAIS
                     IMSQCVLPSFINRIEKRKKNPTKNSWRIDDIEGYAKHVVSFSLAGMAAIGRYNKKHIT
                     SKDVYSPVRSNKKEYASKTD"
     gene            29386..30615
                     /locus_tag="PK125_02805"
     CDS             29386..30615
                     /locus_tag="PK125_02805"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HPB37070.1"
                     /translation="MNIKQSRKIVICILGVLTTIFTLSSCNRSQSGNELQGNSPEVSV
                     VTLQPERISIITELPGRTSPYLIAEVRPQVSGIIQKRFFVEGSDIKAGDQLYQIDPAT
                     YEATYSAAKAALARSEANVISIRNRVERYKELVAMNAVSQQEFDDATAALKQAEADIQ
                     VNKAATETARINLNYTRVTAPITGRIGKSNVTVGALVTANQPSPLAVIQQLDPIYVDA
                     TQSSANLLELKRHIAAGRIKGDGPNQTRVRLILEDGTPYPLEGTLKFSDVTVDASTGS
                     FIQRMIFPNPKYTLLPGMYARALVQEGVAENAILVPQQAVSRDPKGNPLVLTVDASGK
                     VEQRMITVSRAIGNKWNVASGLEQGDKVIVEGIQRVRPGMAVKVVPFDSTQKDSAETG
                     KNANPPEKTTPPPEKAK"
     gene            30631..>32615
                     /locus_tag="PK125_02810"
     CDS             30631..>32615
                     /locus_tag="PK125_02810"
                     /inference="COORDINATES: protein motif:HMM:TIGR00915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HPB37071.1"
                     /translation="MLSKFFLNRPVFAWVIAIIIMLVGVLAIYNLPISQYPPIAPPSI
                     YIGTMYPGASAETVENSVTQIIEQKMTGLDNMLYLSAQSDSAGGSRIELTFAPGTDPD
                     LAWAKVQNKLQLAMANLPEVVQQSGVTVGKATRNWLLIANLISEDGSMDGNDLRDYAQ
                     SNLEKVLARVPGVGEVEMFGSQYAMRVWLNPDKLVDYGITINDVIAALRMYNVEVSAG
                     QFGGAPAVKGQRLNASIIVQSMLKTPDEFATVPIRINPNGSVVKIKDVGRVELGTDNY
                     DIEAFYNDKPSAGIGIRMASGANALDTANAVKAKLDEMSRFFPHGMKVVYPYDTTPFI
                     KVAIWEVVKTLLEAILLVFLVMWLFMGNIRATLIPTVAVPVVLLGTFGTLHFFGFSIN
                     MLTMFAMVLSIGLLVDDAIVVVENVERIMTEEGLSPREATAKSMEQITTALIGIGLVL
                     SAVFGPMAFFGGSTGVIYRQFSVTIISSMLLSVIVALILTPVLCATFLKPVKAGHEPA
                     ENSIRFLRPFFERFDRVFFRIRDRYVKIVGNSFSRRTRYVVIYFVIVAVVGILIWRIP
                     TSYVPDEDQGMMLAQIMLPSGSTIEQTQEVVNAVQKHFRDNEKEAVDSMMTISGSGFS
                     GRAQNNGMVFVRLKDWNLRNRSDLKVKAVAGRAMAAFS"
BASE COUNT         9054 a         7582 c         6774 g         9005 t
ORIGIN      
        1 atctcaggca cagcaacact cgcaaacgtt gcagacgttg cagcaaccga tccgcatatc
       61 gccttaaatg ctgtggcgcc ggcaacagtt gcgattgcga gacctccggg tatatggcca
      121 aggaacttgt gtgcgcaatc aaaaagacgc ttggctatac cagcattaaa tgcaatctgg
      181 cccataagca caaagagcgg tataacggta agcccatagt ttgcaagagc gtcaaagaca
      241 tcgttcgcca tgagccccat ggccgctttc caattgacaa tccacgcaaa acctaaaaca
      301 cctataatgc ccatggcaaa cgcgagttca agaccagtta gaaagaggca taacaataat
      361 atcaggaaaa taacacctat tccgatttca ctcatgattc acctcgcata tcgtctttat
      421 caggccagac aacaacacaa ggcactccac aaatgcgcat aaactcagaa tgtaggcaac
      481 cgggtaatag gggacatgca gggtgaggga aacttctccc ttgtcaaaaa gcatggcgcc
      541 atatttcaac aaattccaac tcagaaaagc gaagaagatt attcctaaca atcgcgtgaa
      601 aaccataaag attttcttta acatcccggg aaggttatca aggacgagat caacgttcac
      661 atggccaccc tgccaggaag tttgggggat tgcacaccct atcacaatgg ctcctccaag
      721 aaaaacgagc tcgtaggtac ctgttattgg cttaccgaaa aaccgtaaga tgacgtctgt
      781 cacggtgata agcatcatca ggcttagcac cacccctcca acgacatcca tcactttact
      841 aatccttaaa attacagcgt gcaatccctc catttttcac ccccggcaat aaatattgcg
      901 gcctattttc tatacggttt cagataatcc atggcaaatt tcagtgcttc ttcgccaggc
      961 aaaccttttt ctttcatgct cttcacatat tcatcaaaaa tcggctgcgc ctttgcaacc
     1021 cactttgcct cttcttcttt cgacagggtg atgattttgt tccctctttt tacaagataa
     1081 gcctttccac ttttttctat gtcatcccac atggctgcgt atttttcaat atactgcggg
     1141 cttaaatcat caattacctt ctgaatatca ggaggcaggg agttccactt atccttattc
     1201 atcactatga caaatacagc agaataggaa gtgccaaaat tctcagtaga atacttaagc
     1261 tgatctccga atctaaaacc ttcggtggcc tcataagcac acatcaaacc atctgcaacc
     1321 cctcttgaaa gcgcatcata tacgtcggtc atgggcattg ccaccggtgt accaccaagc
     1381 aacgacatga gtcttgcatt aggcccatat gttctcatct tcatgccttt caggtcctca
     1441 atcttgttaa tgggctttga ctttgtgtgg ataataccag gactctgggc atgaaaccag
     1501 agaatcttca catcattaaa ttcttttggc ttaaacttgg cgtagaatgc atttgccagc
     1561 ttagtaggaa cagtgttgtt cggataaccg agaggaagat ctaatactga cgtgagggga
     1621 aacctcccaa cggtataacc gagaacgtgg tttcctacgt ctgaaatgcc cttggttgta
     1681 gcatcataag cctggactgc aggaaccaga gtcccaccag ggtaataatt gaccttcacc
     1741 ctgcccttgg ttttttgctc aacttcctta caccagcttt ccaggagtgg ggtctgtttg
     1801 tgtcctatgg ggaaccagtt ggccaccttc agtgttatta ccttatcctg cgcctgaacc
     1861 tggataggaa taaccaatgc agaaagaaat aatgccaata aaagaatctt tgccactgac
     1921 aaccttttca taaaccctcc ttaatgtttt tttggttttc tatgctgccc aatcagtaat
     1981 cacaatcgtt ctataatatc ttgctatggc gttttaaaat attattgtct ctttttcata
     2041 taattaaaag ttaattattt tatttgcgat gcttttctgg tcgcacaaaa atgcaagttg
     2101 aatgccaaca agaaacctgc ccttcctggc tcaaaaacac tggcactaac cgtgacgaat
     2161 agttttccct ttgcactata atgtttcata atggaacatg tgtgtcattc ggagacacat
     2221 tgataccagg ataaaaaaat cctcttgaac aaagaatact gattaagctt tagtgattgt
     2281 ccattttttg ataaagtttt tttctgctaa taccaaggag gcgggccgct ttacttttat
     2341 ttccccctga aatctcgaga acctttttta tgtgttcctt ttccatgata tccaatcgca
     2401 aagcgtcctc gctcagggag ctttttttat caacttcaac aaaagaaggt ataaaaatcg
     2461 tttgcggcag atcttcatgc actatttcgt accggtcagt tgaaataagt actgcccttt
     2521 caattatatt tgcaagctcc cttacgttac ccggccaatg atagtccata aggagattca
     2581 tcgcccgctt agaaactacc ttcttaatgc cttttcttgc atgtttctcc agaaaatact
     2641 cagttaagat agggatgtca tcttttcttt ctctaagtgg tggcacttga attacaaagg
     2701 tgttgagtct ataaaagaga tcttttcgga acctgtttgc ctttatttct tcttcaatac
     2761 ttttgttcgt agcaaatata aagcgaacat ctacggtaat ctctttcgta tcaccgagtt
     2821 ttctaaaaac accagtttca aggaccctga gaagttttga ctgtattgat gtccccatat
     2881 cagccacttc atcaacgaga aatgtgcctg tattggcaat ttgcaatagc cccattttat
     2941 ctatgtgtgc tccagtaaaa gcaccttttt tatagccgaa aagctcactc tcaagaatac
     3001 tctcctgtag attgctcgca ttaatgatga caaagggatt gtgcgaccgt gttgaaaggc
     3061 catgaatcgc tcttgccacc agttcttttc ctgttcctgt ctctccaaga atcaagaccg
     3121 gtatattcga tttcccaaca agggcaatgg ccttctttac cgcattaatc tctttgctct
     3181 caccaataaa tcggtcatgg gcctctatcc gttggagctg ttcttcgagt acgatagctt
     3241 tttcttttaa ctctttcttc tcaaaggcct tcagaataac agcatgaagc tctgacagct
     3301 taacaggttt cgtaagataa tcgtatgcac caagtttcat agatcgaatt gcagtatcaa
     3361 ttgatgcata accagttagc atgattactt ctgcagtagg ctgaagctcc ttcagctcct
     3421 ttaaaagctg gacaccatct atgtcaggaa gtctaatgtc aaaaaggcca atgtcaaacg
     3481 tttcctgtct aacaatggag agcgcttcct taccatttga agcagtaaaa acctccatac
     3541 cctcccttgt taattttttt ttgaacgcct caaggagctg gtattcatca tcaacaatga
     3601 gaacgcgtat catatcaata tattatccca tgctctgcag gtcatctcta tataccggca
     3661 ttgtgacttt aaaagtagtt cctctcccca gtgtactatc tacctcgatg ctgcctccca
     3721 ttttatttat aaattcataa cacaaggtaa ggcccagtcc aacgccctta ccggtttttt
     3781 ttgttgaaaa aaagggttca aaaattttgt ctttcaggcc ttcatctatc ccttcgccgt
     3841 tatcttcaaa ttcgatatag actgaatcat gaaaggggag cgcccttacc tttataacac
     3901 cgcctgctct tatttcaatg gcgtctatgg cattctgcag aatgttcata aaacactgtt
     3961 tcattgcatt gtaatcaccc ctgatatggg gcaagttgga ttcaatctct ttcacaagac
     4021 gaatattcaa cttcttaaag ttaataactt tgaaaacttc ttccaggcac aggctcaaat
     4081 caatctcctt aacctcaaat cctttctttc ttgttaaatc aatcatatct tttatgatat
     4141 tcttacagcg gaaggcctgt tcctgtatga cgtcgagatg ttcacggagc tcatcgaggg
     4201 ctccatcttt gcactcctca ttggcgaggc cctgaaagag atcgcatgca agctctgaat
     4261 tggcagcaat agttgctaaa gggttattca gttcatgggc aacgcctgtc atgagtcttc
     4321 caattactga cattttagca tcctgaatta gttgaatttt tgctctcact ttttctgtgt
     4381 tgtcctctat aatacagaga agcccttcaa cattgccgtt ctttcctcgt aatggaaccc
     4441 cacggaaatg aatatactgg ccctggtctc ccctataggt agtaaaagga aaatcctgaa
     4501 gctcaaaggg ttccccctca agaccctttt tgatgtactc agcaagaccg catctaaccg
     4561 tataaggatt ttcaagccag ttaaacccca caagcttttc ttcagcttcg gctcccaggc
     4621 ctgacagagt tgccaatgcc ggattaacgc tgataaactt tccgttccta tcaatggtaa
     4681 aaattgccac aggagcattt tctatgatcg ctgcgttaaa atcattcagg tgtctcaggt
     4741 cttcctctgc ttttttctga tacgtaatat cctggattgt ctcaataata aggatttgct
     4801 catctcgata ttctttttga agaggcgttg cagtgacaag ataacacttt gtctctccct
     4861 tgtattcact ttttatctca gcatgctctg tacgaccggt tgtaagtgtc cttaaagacg
     4921 ggcaattttt gcatggactt ttgagattaa agatttttgt ataacagtat ttctcaatag
     4981 agtggtgtgc agtatctcct tgaacatgat gaaggaccca cttattcatc cattgtattt
     5041 ttcgcttctg gtcgagcaca aaaacgccca tgggtaatga gtcgagtatt gatatgatcg
     5101 cgttaaattc tactctcggt ccgtttttca ttattctatc cctacttaca tgccaatcca
     5161 tagcaattat tataccacac atatgacatg atgaatctat aagccacaat cagatattct
     5221 gtttgatcaa ttccttggtc tctctgcgta agttagaaat catttttgaa aattagccta
     5281 cttgacaacc atacttttta tcggttattt tattgatgca gggcccatag ctcagttggt
     5341 agagccaccg gctcataacc ggtaggcccc tggttcgagg ccaggtgggc ccaaataaaa
     5401 gcaatatgta tagaagattg gtttacaaag atatttaaac tcttttaaag ggggtgtacc
     5461 attagggcac cccctttaat tttatggagg tagggagttg gaacaggaat taaaaacaat
     5521 ttatgaaaca caaataatag atattcagat tatggagaat gaaaaaaagt tagtttcaac
     5581 accaaaaaaa atcgagaaga tggattctga catcaaagca atgcaagata aaataagcaa
     5641 ggaaaaggaa attctggagg agcttgaaaa agaacgaaaa aggaaagaga aagaccttga
     5701 acttgataag gaaaaagtaa agaaattcga gtcaaaactt tacgaggtga agacaaacaa
     5761 agaatatcag gcgctcctca aggagattga gacagcgaaa ctgatgaacg ataaagagga
     5821 agaagaaata atcgaaatta tggtaaagat tgaggatctg aaaaaagact atgaatctac
     5881 ctcgaaattg ttaaaagaaa aagaaaaggt gacagaagtt gagaaaaaga agttattggc
     5941 tgagattgat tcagtggata aaacgattaa cgaattaaaa cagcaacggg acaatctcct
     6001 ctccgttgta gacaagactc tccgtgaaac atacaacatg cttatatcaa aaagaggtgg
     6061 tatggctgtg gtaaatgtta aaaatggcgt atgtttaggt tgttttatga atattccacc
     6121 tcaacttttt attgaagtaa ccaagaataa cagacttatt ctctgtccaa gctgtaaccg
     6181 gattttctac ttcacagaag catcgcaata gcaacataat ggaatggcat atttatatcg
     6241 acggggcttc ttcctgcaac ccgggacatg caggagcagg actggtggtc tttgacgaat
     6301 tcggcaacga gatcggaaga gattcagctt atcttggtga aatgacaaat aatatggctg
     6361 aatacgaggc gcttgtgaga gcactctcaa gagctttcga agcgaatata aaaagtgttt
     6421 ccatttacac cgattcgctc cttgttgcaa accagatact gggcaagtat aagataaaaa
     6481 atacaaccct tcagaaatat gctgagatag caaagaacct tatacacact ttcgatcatt
     6541 ttgcagtaca atatataccg agagaaaaaa ataagatagc cgacaaactg gcaaaggaag
     6601 cgattaaaag gaaggggtag atgggtggcc gcttcgcaca ggcggagagg aaagtccggg
     6661 catcaacggg caggatggtc cctaacgggg actgggggta accctaagga aagtgcaaca
     6721 gaaaatacac cgccacccgc ctctggcggg agtgaagagt tggaaatgat cggttagaag
     6781 ggaaagccct tcgcaccaga cttcaaactg ccaactccca acgctgaaat gtatgtttcg
     6841 gttggtaagg gtgaaatggt gaggtaagag ctcaccagtt ccgtggtgac acggaagctt
     6901 ggcaaacccc atctgatgca aggccaaaaa gggggctgtt ggaaacagcg gcgaggattg
     6961 cccatccttt aacccgggta ggccgcaaga ggtttagagt aatctaaacc cacagagaaa
     7021 tggccaccat cccgtaatat tgggagacag aacccggctt acagtctgcc cctctcctta
     7081 ccccagaatc tgagaacagg agtgatgaga agcagagaac cggtctgttc ctggccaaac
     7141 cataatagga catggtacta tttcttttta tcataccgct aaacgaacag gaaaaaatga
     7201 acacacaaca ggggaactgt tcgtgacacg gccactgctg gccatctgtg ttatctgctc
     7261 gattctgttc tttctcaatt tgcagtctcg agacttctgg gccccggatg aaggtgattt
     7321 tgctctgatt gtaagggaac tgccggacaa ccacattgtt ccccacctaa atggtaagcc
     7381 ctatggtgag aaaccacctc tttattatta tattatctat ctctcaaagg ctatttttag
     7441 ttggttcaga gacgaagcat ccctgcgact tccgtctggg ttctttgcca ttataggtgc
     7501 ccttgccctc tttttaacct tatacagatt cattaataac catcaggcaa tccttgccac
     7561 atgtatcctc ataagcacac ctctatatta ttggcaggca cgatatcttc aggctgacat
     7621 ggtatttgca gttttcgttt ctttcagcct tcttcttttc ttctggtttt ataagacgag
     7681 aaaggaatac ctcatctacc tgttttttct ttcaacatct ctcgcttttc ttgtcaaagg
     7741 acccctggct acagcgctga ttattcctgt tgttatttta tttctcttct ttgaaaaatc
     7801 attcaggata ataaaaataa ggcaactggc aattggggtc ctcatttgtt tagccgtaat
     7861 ccttccctgg tacatggcag tctatctcaa agaaggggct ccctatcttt atgaaaatgt
     7921 tattcgccag aatttcatac gattctttga tgcgtggagc cacaagagac cattctatta
     7981 ttacttcaaa acattgcccc tggatgtatt cccctggagt ctttttcttc ctctcggact
     8041 tgttcaagcg tttaaacgct ttagaagcaa tagggagatg aacttcttcc ttgtatggtt
     8101 tgtgtggatg ttcttttttc tgtccctttc atcaggaaaa ataagcaaat atatgttgcc
     8161 ggccctgcct gcaatcgcca caataatatc cctttctttc aaaatagaag aaaacaaata
     8221 caacaagatt gtgtttgctt gcattatttt ccttctattc accatgggca tgggactatt
     8281 ttttttcaaa caggacctct atccggaatt tctccctgag cggataatct tcggaagcct
     8341 ttgctgtgct tttgggatgg gccttctact tcttcgtacc agaagaatta tatattcctt
     8401 tatagccatt ttctccttta tgatattcag ctataccatt ggaaatgctg gtaatatctt
     8461 tgaaaagtgg aacatgcata aatcgccaag atttatttct gaacagatga agccatatgt
     8521 tcaggattgt acgccatggg tttactatgg aagtatgcgg ggcgtttatg tctattatgt
     8581 gcaaaaaaag gctattcatg tggaagagca tgacataaca ggattaaaac tcgttgccca
     8641 acaaataagc tctttctaca tactcaccaa gaaaagagat ctcgctgagg tgcacaaagc
     8701 cttggacaaa gtagatatcg tttttgaaga aaagaatcat gattcaccaa tgatttttgc
     8761 ccgctatgct cgttgacatc catcccatgt caatccactg ataccatttt gatgcataat
     8821 gaaaaagttg ggcctttgcc ttgacatgcc atattgtgta tgttaaaaaa agtacaagcg
     8881 ttttaactgg taggctatta tgagtaattt ttttgacaaa ctcaccgtac ggcatcttac
     8941 caaggacgat ctggattcaa tcgtggagat cgacacaaag gttcttggcg aggcaagaag
     9001 ggaatactgg gtcacaaaaa taattaaaga ggctgataca cgaccgtcag atgcttcact
     9061 tgtatccgaa atcgatggca aggttgttgg tttcattctc ggagaggtca gcggatggga
     9121 attcaaagtc cccaacaaca tcgggtggat cgacaccata ggcattgacc ctgactatca
     9181 gaatcgcggt attgctaagg tactcgctac cgccctggtg gccaatctga aaacctacgg
     9241 cgttgataca atttatacgc tcgttaattg gaatgactgg gatctcctac agttttttca
     9301 tgccatgggt ttttccaggg gcgacatgat caatctggtt ttaaaagtat agtatcgaca
     9361 atcacaagtt cacgtctcaa aaaataaaat ttcccattat acgatatatc cggatttttc
     9421 aaaaggatgg gcagttgagt tgactgcgga aagggtttaa ctaactgtta gccctcactc
     9481 actatgatct taagcttttg tttaggaact actttgtcac ctttgaggtt gtttgagagc
     9541 ttaatatcgg ctactccaat gttgtatttg cttgaaatgg atttgagggt ttcccctttt
     9601 ttgactacgt ggtatctcac aggtgctttc ttctttacgc tttccctgcc tatgctctcc
     9661 ttttgagcgc ttttcttttg tgaatagcgg gcaagcctga gcttcatgtt tggagagact
     9721 gtattgcctt ttagattgtt tgccgccttc agctgagcta tatcaatatt gtatttattc
     9781 gatattgatg caagggtttc gccttttctc accacatgaa ttaccgggcc ctgcgtttgt
     9841 tgtcttggcc cagattgcgc caccctgacc tttccactct tagccaaact ctcgcctttc
     9901 acaggttttg gttcgtgact ctctatggcg ttttttgcaa gttcggtttt tctttttgat
     9961 gggcctgaaa atcgtgggag attaataacc atacccggct taattctcaa ctcaccttcg
    10021 cactcgttaa ccaaatagag gtcatcttga ttaagcctgt atttcctggc aatagaagcc
    10081 agggtctctt tcttcttcac tttatagctg ataacatctc tgatctgtct ctcgtggctc
    10141 attgccacct gaagcctact ggtaaatgcc tgaatatttg taccctgtgg caattttatc
    10201 ttgtagtcat ccatgttggg aggggtaatt cccctcagaa tttctggatt gattgaccgc
    10261 acatctgcaa ggtctattga tgcggatttc gccactacag aaagcggagt ccctcttgga
    10321 accctgacaa gttcgaacct tatgggcgca tcataggtaa tactcccgaa accatatttt
    10381 tcaggatctt tggctattat tgctgcggca ataagctggg ggatataggc ccttgtctct
    10441 cgcggaaggg cattataatt agaaagctcc cagaaatcgt tcgtattgtg cttttcgata
    10501 gcgcgctcaa cccgtttctc acccgcattg tatccggcag ctgcaagata ccagcatccg
    10561 aattgattaa aaagatccct taggtacttt gctgctgcaa cagttgattt ttcagggtct
    10621 cttctttcgt caatccaata atttactttc aacccatatc tgccgcctgt tgcatagatg
    10681 aactgccatg gtccgcatgc cttggcatgg gagtatgcct tagggttaaa accgctctca
    10741 atcattgcaa gatagacaag gtcttcgggc atcccctgat ccttaagtat ttctcgaatg
    10801 attggcacat agtgtcttga tctcttgagc cagttaccaa aaacctttct cttttctgtg
    10861 ctgaaccact ggatataata cttaacagcg tcattaaata cgataggaat gtcaaaatcc
    10921 tggaattgtt cgggttcgag aagaatggtg atatcatcct catcgtcttg cgtcaaatat
    10981 tcgaccttca ctgagcccac atcattcttc agaacgaatt cagatttttt aatcggctca
    11041 acctttgcaa ctacctgcgg ttcactggtc ttctgtgata ccggccgccc ctgctgatgg
    11101 ttcgttggag ggctttgctg gtaattaacc ggtgttgttt gttgggccgt tgaacaaccg
    11161 aacaagatga ctataacgaa aaaaagtacg acgaatcttc ccatgatctc cttacattaa
    11221 gcaaaaatta tacctaactt tatgtaattg atattaaaaa ttatgttcca acaatgtcaa
    11281 caaaaaaatg cgctttatca atcatattta agaactttcc cctccaaccc tctgtaacct
    11341 ctcgaaaaat cgtacgcttt catgcgcttc ctgttctgaa gctgaagctc cctgataata
    11401 aaaccatctg agccggtaca tacatgaatc ccttccttag atatagcaaa aatcgtacca
    11461 ggaattgtat gacgctcaac ctgccccatt tcaccaacag ccacactccc ttcaagaacc
    11521 ttcaacaaca gaccatccaa aaaggtgtac gctgtcggcc acgcaataaa ggctctcaca
    11581 tgtcgaagaa tctctgttcc gctcttcgac caatcaattc tgcccatctc ctttgtcaga
    11641 atcggggtaa atgttgcctt ctcgtgttcc tgagttaatc cttcatggag gccaactaaa
    11701 gctgtttcac tgagaaaatc aggcagtatt acagcggatc ttttggaaag acgatctgag
    11761 agggtaatgg cgttatcctc cgggcccaca gctatggtct cctggtagag gatgttgcct
    11821 gcatccatct tttcattcat ccgtataatc gtgactcctg tctctgagtc accgctcagg
    11881 atcgtccact gaattggtga cggtcctcga tacctgggca ggagtgaagg gtgaatattg
    11941 atgggtccga tagaaggaat atcaagggcc catttcggga taatgaggcc aaatgacgca
    12001 accacaaaca ggtcaggcct cagcctctca atgtccttta tcgcctcatc tttgaaggaa
    12061 tcgatttcaa tgacaggcaa ttgaaggttc attgcctctc ttcttacctc ggtatcttca
    12121 aggacatatc ctctcccttt tggttttgac ctgcgggtga caacacacga aacaaaggaa
    12181 gcaatagact gtaggggtgg gacagaaaaa ctcgaagaac cgaagaatac aaccttcatt
    12241 ttcggtcttt catatatttc ttcttgaaaa ggtccttctt taccggactc aaccggtcta
    12301 tgagaagtaa cccatttaga tggtccagct catgctggat cgctcttgca agaaaccctt
    12361 cgcattccat ctcgaatggc tccccatcta tattgatgcc tcgaacaaga accttcttgg
    12421 cccttttaat atattcataa aacctgggga gactgagaca tccctcttcg gatatctctt
    12481 ccgaatcctg ttccacaatg accggattta tgagaataat gggtttactc tctttttttg
    12541 tgcggggatc cagcacggca acacgagatg agacacccac ctggttcgcc gcaagtcccg
    12601 caccctgagc aaggcgcata gtctccacca tgttattgca cagaatcacg gtctcgcctg
    12661 taatagactc gattggttca cacgcctttc tcagtatggg atccggataa gtttttatct
    12721 gtatgtaggc catggctata tttgtaaacc aaaacgccgt ctcagtcaac cccacactgt
    12781 caggaaacag ccgcagatca ggccctgtac cagggtttac ttgaagctga ccgatatctc
    12841 tacaggccct tccgggctca ggcccatgta actacctttg attgttgagc ctgccttagc
    12901 aggaatcatt tttgttacag aaccaaggga caggatatct ccttttttta acgcctttcc
    12961 ctcagctacc agtgaatctt tgatccatag aacaacattt agaggatggc caagaattgc
    13021 gctgcccttt ccttcagcaa gatcagcgcc tgtttcatct tttagagtca tactgaaatt
    13081 ctcaagccgt tcttgccaat cccgcgtggg agaaagttgt atgggattgc ccatgatgaa
    13141 ataacgggca cctacgttaa ttgccgctat tgcagggcca gtaaacttga caccctttgc
    13201 aaaaaccatg tctgctgcct ctatagcagg tataacagca tcgagactct tcaacgcttc
    13261 ctcggcagtc tttgcctggt tgatatcatt acttccaacc ctcacgcaca gatcaccttc
    13321 gatcatggaa acagcaccaa aagccgctgg caactcagct ccacttttga gcatcatctt
    13381 ggaaagaagg gtgcctcgca ctggattcgt gacaccaaac gctttttgca cattaggatt
    13441 cgtaaggccg gccttgtatc caacagcctc cccgtaatcc ttgctcagat ttgccacata
    13501 cttctcctgt gtcttcattg cctggtccat ggtcatctgc gggtcgaatt ctgctacagg
    13561 aacttttttt tgaaaatgct cagtaagaat atcggcaaaa tcggcagcac cagcacatga
    13621 aaacccctga gcgcagaaaa aggcaattcc aataaaggaa catacccaga gccgtacaat
    13681 tatatttttt ttcattgccc actccccctt ataagtttat tttttaatgc tggatattta
    13741 tccaaaaaag tctatcacga tcgattgggc tcttccatat tattttttcc cgaacaccaa
    13801 tttgccggcc taacaggttg ttcgtaacgc tgtattctgc ttcttgtctt ccataataca
    13861 acatgtaata tccattatga actggctatt actgattttt tttccttgaa gagatataat
    13921 tttacgatga aatatcacat cggaacctcg ggttggcatt acaaacactg gaagaacata
    13981 ttctatcctc agtatctcaa acactctgac tggctctcat tctacgcgca gcattttgat
    14041 actctggagg ttaacttcac cttttatcgg gacgttaagc catcaacgtg taaaaaatgg
    14101 tacagcacgg tgccggaaaa ttttctgttc tctgtcaaaa tatcaaagta tattacccat
    14161 ataaagaggc tgaaggttga cactcattcc attgaacgtt ttattgaaag cgtccggtgt
    14221 ttgggagaaa agaccggggt tgttctggta caacttccgc cagagcttgc gtttgatgag
    14281 ccgctgatgc gttatttttt cgctcttctc gacagaaagc tacgttatac tgtggaggca
    14341 agaaataaat ctttcattga tgatagattc tttgagcttt tgaaagaaaa caacattgcg
    14401 tggtgcattg cagacagcgc aggacggttt ccttaccatg aggctgtcac agccccattt
    14461 atctacatac gactccatgg ttcacaaagg ctctatgcat ctgactacag cgatgaagaa
    14521 ctcaaggtat ggaaggacaa aatccttaac tggaacagag atatttttgt atacttcgat
    14581 aacgattttt atggctacgc agtaagaaac gctttgaaat taaaatctct tcttatcagc
    14641 tgttaggcac tttcacaggt ttattaccat ctccttcttc atctcgagaa tagccacttc
    14701 ttcccattag aatcgattgt ctccgaaaag atggatccgg cacaccatac aatcgttcag
    14761 gaatctacag gatgactatt aactctgcta ccatgatcat tcccggtgca gagcggttct
    14821 ttcaccctat gtactctgta cgggtatctg gaaaaaaaga gatttcctga tcggacaaca
    14881 actcttatcc tgatgttcaa taaaccaaaa acggtcaggc tggcatatac attctcctac
    14941 actgtacctg gaaatatctg aagtactgga agaggatgtg caggcaagta ccacgggcag
    15001 ttaccctgaa agggaatgag gaaaagcatt gaagataacc cgtctggccg cttcaatatc
    15061 agcctcatac cttgcggaaa gcctctttgc atttctcatt tttttctcct cagaaaggtc
    15121 ctgtacgtag tttagaacac ctccaggaca ggttaaattt cccatggtgc cgtgcccttt
    15181 ccgggcacag cagaagtatg ggcagtctaa ttttcgtcct tgaccgcctt gacaaatcat
    15241 tcttcgatgg tacaatgtaa agtccccaac cagaaatgca attaaggagg atggagtctg
    15301 gcacgggcgt tatactatat tcttattctg accagtattt tgacagcatc ttcatcatct
    15361 gcttcactca ctagtagtga agcagaaaaa gtgaagcagg tgttccgggc aggcggtttt
    15421 ttgacagagg agattgattc cctccttcag gaccagcggc tggctctgta tcctgaaatc
    15481 ctgaatcaga aaacaaaagg aatcaactat cttcaccccc gattcggcct attaagccgg
    15541 tcatcaatca aaagagggca gcaggtgctg aaggaaaaca gaattactct gaaaaacatc
    15601 gaggcctcct atggaatacc ccgcgaagta ctggttgcca tttttcgctt ggagacaaac
    15661 ctcggcagca acctgggcca gtaccgtgtt tttaacagcc tgctgaccct gacggtaata
    15721 gtaaaccgcc gctctgtatg ggctgctcaa gaactgttta gctttctcac gctgtgtaat
    15781 agcaaccgaa tcaacccttt ctctataaaa ggatcctcag caggtgcttt cggtctctgc
    15841 caattcgtgc cgtcctcata tctcgcttat ggcattgacg gcaacggtga cggtgtagtc
    15901 gatctttttg acttctccga tgctatggcc agcactgcca acttcctgaa gtcacacgga
    15961 tggcacagca gaaatgctaa gagacaattt cgagctatcc tggcttacaa ccattgcgag
    16021 aattatgcaa aagcagtgct cacatattca aaacaggtaa ggctgtctca taataaaaaa
    16081 cctaacacca ccaaaataaa caacagacac cagaacaggc agaaaggaaa agactttgtg
    16141 cgcatatagt caccgtgtac cacatcaacc atatatagtc tccaaaaagg ttccagctac
    16201 atgtgatgaa attctcactt catgaggcta ttgttaaaat atagactaaa aatgaaggct
    16261 ctttctccag gcttaccagt tgatgaatac gtcataattt actgacagat tacagaataa
    16321 aacaaaagat tgataaatta gctcagattg tgcaaaaaac cacacagcag gtttcactat
    16381 tctcagctac ttgacatctt gtctgaaagg gtattacccg gctgagctat gctgtgccct
    16441 gctcaacaat gacctttgag attctatact ccaggggtta tcgcaggggg gtgcaaaaca
    16501 gcatggtgtg aatgttcttc cagaagaagt tagtcgaatc gtatggaaat gctcacttga
    16561 agatggcgtg cttcggattg gcttcagata tgttgggctc gtaggcgaag aaatagataa
    16621 cagtatcgtt agggtgagag aaacatgtct tttcgtcacc ttgtagggcc tgccacctgc
    16681 acgggacttg atatcgagtc attgaaaagc cttataactg tcggctcatt ggaaacattg
    16741 tatcggttag aggggcaatc cctctcggat ataatgacta tgcatataga gggagggttt
    16801 gcagggacgt gttttagcag acgggaagct cctcctgtct ctgaaatgag tgttgcagaa
    16861 aaggtacttg ctgattatac ttttcagagt ttttctgcct gacacattat tatacattta
    16921 tacagaaaac tgcttggtac ataaagagca gtaagaatca aagatctctc atacagataa
    16981 taaggatctt ctattctcat tggcagatac tgggtgtacc gccagattcc tcccgctgca
    17041 aagggttttt catttttcac ccttgaggaa tgaggagggg cttatcaata caatcataaa
    17101 acgcataatt accaaaagtt ctgttgttca tacaacgtga acctccgtta ctggtaaaaa
    17161 gcaccggtga aaggaggagg caagggttga tcaacataaa agtccattcg tttcaattac
    17221 acaaaaggtt ttattctcct tctgaaaaca tactgtcctt tgtttcatgg atgtacaact
    17281 tcctcggtat tagactcaac gcagattgta aaaccgtttg aaagcctgaa aaagaggctg
    17341 caaatgaact taattttgtt ggcaaaggta agcccaggcc acggcaaaga tagccactgt
    17401 tttttcgaca tttttgtaga attttgcctc acaacgacaa aaatgacgac cattattact
    17461 taaaccggtt attttccagg ttatttcaca ggaatttaaa cacctagtcc cggcataaat
    17521 attgctgttg actcttaaga gacctataag ctatcattga cagaatatta tgaagagtac
    17581 ggatcaagat tattgatagg agggacaaac ccaatgaaaa cagccataat gattgccctt
    17641 gccttaatgt tgctggtggg ctgcaacaat ccagccccaa atgtacctga acctacccct
    17701 agcccgcaac ccagcgtacc tgaaactcct ccaagtccac caccgagtgc acccgctccc
    17761 agcgctccac caagtgcacc tgctcccagc gctccaccaa gtgcacctgc accccctcca
    17821 cagcctgctg tacccgaacc caccccaggc ccgcaagcct acgcacctga aaccataact
    17881 gaccaggaac ccggcatacc ttatcctgtt tccagtcctc cgcccatcga gtttgcagca
    17941 ccaccagatg ttatagtgtt gcctgataca ccgaatgttt acgttgctcc cgggatcgct
    18001 gtagacctgt ttttctggaa tggctggtgg tggcgtccat ggcggggcgg ttggtatcgc
    18061 tcgcggtatt acgaccgcgg ttggggctat tatagcagcg ttccgagttt ctattatgat
    18121 gtagaccccg ggtggagggg atattacaga aaccgtagtt ggcgtggtca cagatggaac
    18181 tatgaaccaa ttcgttaccg atatcttcga gaaaactgga agagatggca tgataataga
    18241 tactgggaaa gacagaggac ctggggcgtc cagggttacc ggccaccacc cccggcaaaa
    18301 aggcaagaac tcagacaaca gagggaacgg gactattttc agaggcgtga ggttcagcag
    18361 cacagacagc agatgcagca aaaacgaatt cctcagggta aagtacagca gccaccacct
    18421 caggggaaga tagagcgccc aggtcatcag ggaaagattg ctcnnnnnnn nnnnnnnnnn
    18481 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    18541 nnnnnnnnnn nnnnnnnnnn nnncagttgc acctcctcct cagggtaaag tacagcagcc
    18601 accacctcag gggaagattc caccatcagg accaccacct caggggaaga tagagcgccc
    18661 aggtcatcag ggaaagattg ctccagttgc acctcctcct cagggtaaag tacagcagcc
    18721 accacctcag gggaagatag agcgcccagg cccacgggga agggtttctc cgtctgagcn
    18781 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    18841 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnc aggtcaggga aggagtcctg
    18901 aaggaaggca acggatgcaa cggaaagttg aacaaccagt acagcctcag gggaagatag
    18961 agcgcccagg cccacgggga agggtttctc cgtctgagcc accaggtcag ggaaggagtc
    19021 ctgaaggaag gcaacggatg caacggaaag ttgaacagcc agtacagccc tgatgaacag
    19081 gtgaaggagg agaaacagaa caaatagaat gataaattag attagaagag caaaagagca
    19141 gtgtcagaaa tggccctgct cttttgcttg gctcaatcat cactggacag taccttaaca
    19201 accctattac tgaaaaaaaa ataagtgttt tataaccgaa gccatatgag aggccctgtg
    19261 gagggctgct ttttcagtga aatcctggaa tttgtctcgg gggccatcgt cattgcagaa
    19321 agcatttgga cctacttcac acagacaaca ctgaagcaaa tatagacatg aaaactttaa
    19381 ataaataacc gtatctatcg aagcagtccc ctatcaacag ggaggcttgg tcgcaacagg
    19441 ctgtatcaat cataaattga ctgttgctag gccctcatca ttcaagctga aaacaataaa
    19501 actgaaggtt ttctggtgct cttgggataa ttggtgtttt acggcaatct cagacaattt
    19561 atgaacatct gggaactgga ggcaatggtg cgagtcatcc tcacaatcta cctgttgcat
    19621 acacatagca taaaaattct tgtcgttccc tgcagatgag tgtagaattc aagatgggca
    19681 aattcacata ttatagtgtt tgggacaatg aaaatcgtgg aggaatgcga tgacacaacg
    19741 gattctgagc ccggagttaa gaaaacaata cgagtctgtt cgcgacttac ttgaggcttg
    19801 tctcggcctt actgaaaaat gttcagacat ggaggcttac aggattgtcc aggacagatt
    19861 atctcatctg cagtcagccg ctctttttgt cattgtcggg gaggtaaagt ccggaaaaag
    19921 cagctttgta aatgcacttc ttggtgagga tatctgcgac gttgcgcctg acccgtgtac
    19981 agccgttatt caagaactgg tatatggaga agtccgagaa aagaagtctc ttggcgataa
    20041 ttgggagcgt gtcagtttac cgaaaaacat tcttcaggaa atctctattg tagacacacc
    20101 agggacaaac tcaattattc gagatcatca atctattatt gaaagctaca ttccccagag
    20161 cgatcttgtt atattcgtct ttccagctaa aaatccccac actgcctcag cctgggattt
    20221 tctctcgctt attcgacagg attggcatcg gaagatagtc tttgtattgc aacaggcaga
    20281 tttagcaacg caacacgaac ttactatcaa tcaggaaaga gtaaaacaat atgcaagagc
    20341 gaggaatgtg caaaatcccg tagtttttac cgtttcggca aaacgggaat ttgaaggggc
    20401 aacagacagc ggttatgctg agtttcgtaa cttcctgacc agggcaatcc agtctgggga
    20461 agtatggaac atgaaaatcg aagggtcacg agatacggca aaaaaaattg tcaatggtct
    20521 cattgcacgt cttcgaagcg aacagcaggc tattgcagac gacatgacat tctataaaaa
    20581 cctgctttcc aaagtggaag cccgccgcga aaaggctaat gcactcaggc gtctggctgt
    20641 agatagcctc tgcatttctt acgataggct tgcatccaaa ttggaagaag attttacaga
    20701 aggtttgggc ataggaactg tgctcctcag ggctatccca attttacgcg ataaagatgt
    20761 taaaacgtgg ctaaaggaac tccaagccaa ctttgaaagc acctcgaaac aggttattga
    20821 tacagaatcc atgcgagtat caaaagatat ctcggatgaa atcatgctta tgtttagcga
    20881 attgacagat gcaattgttc acagacagag caccacccag gaagtttttt caacgaaaga
    20941 ccatgaccga accgagattc ttacacatct gcagaaacag cttcaagaac ttcgtattgc
    21001 agacatagtg accaacaagg ggattcaagg ttctgacatt ggaaagttat cactggctgg
    21061 tggaggtatt gctgcccttg gtgctgtaat tgccttcgct acccagttag tagttcttga
    21121 tattacaggc ggcataatag cattggtggg agctgggctc attgcagtta cgttgatctg
    21181 gaagcgctca agtattctcc acgatttctc acaaaagatg aacaaatcgc ggagcgaatt
    21241 ccgggaacgt ttggataaag aaattgcccg aatctttgaa aaacttttta tggagattga
    21301 acaccgcctg gaagaaccac tttctcgcct taatagtaag gcaggtcaat tgtcaattct
    21361 tgtggcagaa ggggagagag ttaatgaact ttccaaatca atgtgagtaa tggacattct
    21421 aattccatgg caattttttc ctctaactca atcctgtaac tctgacatca cattcgtttt
    21481 atccttggaa ccccctcttc tttaacggtt acctttggcg ctgaggccac aacaagtacg
    21541 ggaacatccc tgggacagaa tcaattacgg attctcactt cgtcaggatt gattgaaaga
    21601 gtgggtagag ttttgtaaga tccaggtcat ccttcaatcg cgtttaaagc cctttgaccg
    21661 tcttgggagc caaagttttg atcacagatc tgccatagac caccaaccac catctggctg
    21721 cttattttca gcacctggcg cacggcgtgc gacctcaata agtctcttaa ttttttcttt
    21781 tacaggcaaa cgattctaaa atcgcgtcta tgtatacaat atccatcctt tttacatttg
    21841 gcttcatcaa ctttttgcat ccccatccta ctacgtcatc ccatgtgatt ttacagatat
    21901 tcagtaaaaa tccctcttgg caaacctatc attgccacat tcttcctgtt taacagggca
    21961 caaaactctc agatttcatt tctgaaatct tcctggagat aaccctgtca agctcttttt
    22021 tttaggaaca aggatgccag ggcacctgcc agagaaacag cagaggatag aataaaggcc
    22081 ccggtaaaag atcctgttac atctttgatg tagccagcta ctgctggccc aaatgcctga
    22141 ccaataccga aaaataacgt tatgcaacct aaccctgcag gtgccagctt tggacccact
    22201 gcatcaccgg cagcagcagc aaggataacc ggtatagagg aaatgctgaa cccgtatata
    22261 atacatgata tataaagacc tggaagatca gtccagaatg cgaacatgag ataggataga
    22321 gcaaggacaa cataggcata catcgaacca aatcgcctcc caaaaaaatc agacacactg
    22381 ccccataata ccccgctcga tatgctgcaa aggccaaggg ttgcaaacat ccatccagca
    22441 gttttctcag aaaggcctat ctctcgtgta agatatgcga taaaaaaagt tagatagatt
    22501 atatatgaga aaccatacat caaatatact ccgccaagct tccagacttc cctttctttc
    22561 atcacatcgt gccaagctga aaggatcgaa acccctcctt gtctgtccat ctgactattc
    22621 ttttcatctc cagcactacc atacatagaa agcccctttt ccgcaggggt gttcctctca
    22681 agggcataga ccacaaagga gcccacaaaa actatgatac ccatagcata ccaggcatac
    22741 ctccacccct catgtccgta atttacaata aaatagggga ggataatccc tgcaagggaa
    22801 agccccatac cggttcctac ggtggcaatg cctgttccaa gacctcgctt tctgaccaca
    22861 aaccatgcgg caggtaaagc cataacaggg atataactac cgccgttacc catgccggtt
    22921 ataaacctca tggcaaaagc gaatgcaaat gagcctgata gaccggtgag gaaaaggcat
    22981 acccccatga ttagaagaga aacaaatata atatttcttg ccccaaaacg ggcggcgaga
    23041 aaacctccaa ttactgcaag gcacagatat ccgatgaaat ttcccgttcc gataagacct
    23101 acttgtgtgt aggttaaggc cagtccgtcc ttcatgggcg ggaggattac agaatatgac
    23161 attctcccga aaccatgagc aaggaggacg acaagagccc ctgtccaggc aataacccat
    23221 gcataatgtc tcttcatgaa cctaatcctt atcttttagc atatccggcg aaagcccgat
    23281 tatcaaaggt tgcgtgaaaa taaaaacagg tgtgcatagc catcatgata ggcatccctg
    23341 atctgcagcc tttttatacc tttccaataa gatgtgtttt ccccagctat gagttcctca
    23401 gccttcaccc attaaccagg ttacgcccca tcaagactat ggtgctcgtg tgcgcgatac
    23461 cagtcagccg gactcagaaa atattcttcc acatctttta aagaaagata atgttcgtac
    23521 tcgattgtag cgaggagatc gaaaaaatct ttttctttct tgtcagtgac agcgtcacgg
    23581 agccttctgt agaattcagc acctttctgc tcaaaatcaa tagcggtcct gattgcctgc
    23641 aggtcgtcat catcgcttgc agccgtctga tccacctttt gcagaaactt cgtcaaaatc
    23701 tcttttatgt ctgtttcctt tactatcaaa ggcagggttt caggccagat tcctttcttc
    23761 tcccactcta ttttaagttg tttgagacgc tcgtaatgct caagctcatc gtcagcgatc
    23821 tgtttaaaca tggccttacc aagggcattt tttgtccttt cagcattttt gagatagaat
    23881 tctctctcct tcatttcatt attgagggca acttctaacg cattcagccg ttccttcgta
    23941 tccataggta cgctcctttc tcaatagtca gttatttttc agatacactt attaaacact
    24001 atcttccgcc ttcaggtatt cgcggagatc caggtctttg cctcggatgc tgtacctggg
    24061 gttatctcct tcgctgttga tactggcgaa cctctggtct tgcacggtat tgccgctctc
    24121 tttcacgtct gagctcctgc ctctggtttg ccggcttggg gcggtactgc cagacacccc
    24181 aggtatgctt tctttgccag tgctgattgt tacgccagtt ttgccactgt cgttgtaatc
    24241 gctggtggga aattctctca tagtaccagt tgtgaccata ccagttgcgc ttcgtgtaat
    24301 atcctctcca ctccgggtcc acatcataat agaacttcgg gacacttctg taatacaccc
    24361 acccacggtc ataataccgg gatcgatacc accggccttc ccaaggacgc caccaccaac
    24421 cgttccagaa aaacagatct acgttgaggt cagggacaac atagacatca tctgtatcag
    24481 gaagtactat aacatctggc ggcacggaaa atgtaatagg tggtggtagg ccgatactaa
    24541 tacgaatgcc cacttcagac cttgcatatc caggggctaa gctaaccaat accagtagta
    24601 tcactcctaa aagtaacttt ttcatttcat tccctcctta gttttgtgat ctatcctgtt
    24661 gattgattcg atatcaccca tgccgatact atgatattat agtacgattt tgccatcctg
    24721 caagtatact ctgactcaac taattctgga acaaggcccc atgcaatggc atacggtgtt
    24781 ctctccaacc tgttttattg ggatatattt ccgcatttga gggatgcaga ttcagccagg
    24841 gcataccttg tgtaaggcta tttaccggcg ccggcagata cctgcatacc aggtgcttcc
    24901 atatactggc ccttcgttgt ctgatggcgg aatattgctc tgtggcaata aaacacttcc
    24961 tggttttcct atcctcctat ggatttaccc tgttcgaaac tgctcagaaa atcctggaat
    25021 ccagcctttt gcttcattgt ttcccggtaa catatccagt aagcctacgg cctgcttcct
    25081 ttttttctga atcgatacat tatatcattc caaatcaatc ttatgacacc ttttctccac
    25141 ctacaaaccg gtagattgtt gcgccaataa gggcgcccat gattggagca acccagaagt
    25201 gccataactg agcaagtgcc caatctccaa caaaaactgc cacgccgatg ctccaggctg
    25261 ggtttacaga agtgtttgtt accgggatac tgatcagatg aatgagtgta agacataacc
    25321 aaatagcaat gggagcaaaa ccctgtggtg catgcttgtc tgtagaacca aggatgacag
    25381 gcagaaacat catggacatc actatttcgc atacaggagc agcaagtaag gagtacttcc
    25441 cgggactgtg atcgccataa ccatttgaag caaaaccggc actcacatca aacccagcct
    25501 tgccgcttgc aataaaatag agaatggcac ctgccacaat tccgccggga acctgtgaaa
    25561 tgatataggg caggaggtct ttagcgggaa acctgccacc cgctcaaagc cctctggata
    25621 ctgccggatt gaggtggcaa cccgatatat gcccgattgc gaatgccatt gtcagcacgg
    25681 taagtccgaa tacgagcgaa acacccagca gaccgatacc caaatcggga aatgcagcgg
    25741 ccagtactgc actaccgcat ccaccaagga caagccaaaa ggtaccaaaa aaactctgcg
    25801 ccatattttt tcatgattct cttcctttaa ataagataaa aatttcgttg aagtcatgtc
    25861 tttatttctg aggtgcagtt cgggcatctg gtggccttga ttggaataac ggagaaacag
    25921 aacgggcaat ccttcgtggt ggggtcgggt gggggtggcg ccttcctgaa cctgtttacg
    25981 ttcttgataa ccaaaaacac tgaaaatgcc acaatcagaa agctaataat cgtattgata
    26041 aagacaccga tgtttacggt aaccgccccg gcagccttgg ctgcagccag gctggcgtat
    26101 ggggctgcca ccttgccttc ctttatcgtt aagaacagat ttgaaaaatc cacattaccg
    26161 agcagcagac caatgggcgg cataagaaca tcatcaacaa gtgattttat gattgtaccg
    26221 aaggcagctc cgataatgat tcctactgcc atatcgagca catttccttt catggcgaat
    26281 tctttgaatt ctttaaacat ttgcatcctc cttttttatc ttatctgttt atgctgattt
    26341 ttgtttgttt tcatggctca agctttgtct tgttttcatg aattaaaaca aaacttggca
    26401 gcctcatttt cccctgtcgg aaaccatagg ataacctgcc ctgcttactt agataaagca
    26461 gggcaggctc cagggggttg ggttagttgc gatccttttt cgccggttta gcaggggcag
    26521 gacatggcgg gcatgcagga cagggcggac aggtcttttc gggcgggcaa acaacagccg
    26581 gcgggcatgc tggacagggc gggcatgctg gagccggcgg tacaacctta gccggtgctg
    26641 gtggcggggt ttcacccttc tttgcttcct ggaaacaacc gcatcctcct aaagataatc
    26701 ccaaaacaag agtcaatgcc agaattacta caaaaccatg tctcataaat atgtcctcct
    26761 tttgaaatta tttgataatt gcacataacc cacatagcaa atccaaaaac gtcttttcat
    26821 tttaatacca ttaatcacgt gttaaatgaa agcagggtgc aatagttgca tcccgcagca
    26881 aatttttgct aactggctta tcatagaatt catatctgtt gcacgctctt gccgtttcaa
    26941 attgactatg cgcacaaggt ctgagcaagc caaagactct ccattcatgt catctgcgaa
    27001 taaagccttc gtcctttgcc ttcagtcttt tatttgagcc tcttaatctg tttttcgcct
    27061 tcttgtgccc tgctgtatcg cgaaattatc ctgctattcg accccggctg gttgttgtca
    27121 gcgtcaaata aaacttttaa agctttatca ggctcatgag ccgctcgaat gcctccatgg
    27181 gaaagggcaa ttctgtcttc aagtaaacag cggtatcagg atagcacaac aacctgccca
    27241 ctgcttcccc ggctgcctga ccatcattga gatgggtgaa tgtaagaaaa gcattacagg
    27301 cagctccgga ctcccgtgga atgagttcaa gaatacggaa tgtctcatcg agccaataac
    27361 agatcgcccc aagatatgtt atggcatact atctttttat tgcctgatat ttccctggat
    27421 cagtctattt tcacttatga tacttgaatg aaacattcag tctcacccgg taacttgtca
    27481 ctttcccgtt ttctactgtt acatcctgtt taaccacctc accgatgcgg agatccttga
    27541 cgctttttgc agcggtttct atggccagct ttgcagcgtc ttcccatgac tttgtgcttg
    27601 tccctacaat ttcgttcacc acatacacac tttctgccat gacagcctcc ttttgatctt
    27661 ttcttgtttt aaaagcttat atgttgttaa tagcataaat aatatacatg tcaagccaat
    27721 ttttctgcaa ttgagtacta ataatacccc tgaagaaacc atgtatctca aatgagctta
    27781 actgaatact cattcgatgt gtgatttagc tacctgtatg gcaaggtctt tatccttcgt
    27841 gaataatgtc cactccattt cattagagtg aataccggcc ctcttgtctt ggcccctgct
    27901 ataataccag tggccaaact tctcaactgt agaaaaggga tgattgcctg tgcaaaacat
    27961 gccgcaaggg ttgatctctt taaaaaaaat tgtttcctca gggtcttcgt tgtatgcaag
    28021 ctcatcaggg agtcttatgt gagacacatc taccccctct gcaacctttt catctaacca
    28081 atcggatatg ttcacagatc tttcgctcct tatacgctat tggaactgtc ttcctggatg
    28141 gcgtctttta cctgcccttc tcttagggct tcctttcctt tctgtgttaa ccagcacccg
    28201 tcgtacacgt cacctgccac tgtccctcca agcccttttt cgacaagccc ttttttgcac
    28261 aatccctcaa gtattaacct gtcagacttg tttcggcaaa ccttatcaat aaatgtggct
    28321 cgcctgtatg caccaccggt gaattgatta ttctcaattt ttacaagcat atctttttct
    28381 ttcccattga tttcaatcat gaaatacccc ttttctcatt attattggcc tcataactgc
    28441 aaatgtcaaa caaattcaga gagagcctta catcctttat tatacaggtg atttctccat
    28501 tgaccattta agaaattagc ctgccatccg aaatgaaatt tatgatcttt tctattgaca
    28561 atttaaaatg attatttaaa ctgtatagct acagttgtcc cggaggatgg catgaagact
    28621 ctgcgaaaag atacagcacg aactcaaaaa aatctgctca tagcggcaag taagattttt
    28681 gcagaaaaag gatttcgaga cacaacagtt gcggaaatca gcaaacgagc gaagacaaat
    28741 ttagcagcta taaattacca ttttggcgac aaagaaacgc tttaccggga agcatggcga
    28801 cattcctttc gtgagtcaat aaaaatttac ccgcccgatg ggggggttga tgaaaaggct
    28861 tcccctgagc accgtttaac cggacgagta atggcgctgc ttcgcaggat tactgatgac
    28921 ggaaatgcag agttttcaat cgctcaccag gagcttgcaa aacctaccgg attattggaa
    28981 gaagttatgc accaagaatt tgagcctctt cagcaaagat ttgaatctct cgttcatgag
    29041 attcttggtc cacacagttc caagattcag gtccggttct gcgctataag cataatgagc
    29101 cagtgcgtcc tcccgagttt cattaacagg atagaaaaac ggaaaaagaa tcctactaaa
    29161 aactcctgga gaatagatga cattgagggt tacgccaagc atgtggtatc tttttctctg
    29221 gccggaatgg cagctatagg gcgttataac aaaaaacata ttacctcaaa ggatgtttat
    29281 tcccctgtcc ggtcaaataa gaaggagtat gcatctaaaa cagactaagt atgaaatatt
    29341 acaacctttt ccatataggc aaacgttcaa gtcagagagg tttgtatgaa tattaaacag
    29401 agtcggaaaa tagtaatatg tatacttgga gttctcacaa caatcttcac cctgagcagt
    29461 tgcaatagat cacaatcagg gaacgaactt caaggtaatt cccctgaggt atccgtagtc
    29521 accttacaac cagagagaat ctcaattatc acggaactgc ccgggcggac atccccatac
    29581 ctcatcgccg aagtgagacc ccaggttagc ggtatcatac agaagcgttt ctttgttgag
    29641 gggtctgata tcaaggccgg tgaccagctg taccaaatag atcctgctac ctatgaagca
    29701 acgtattctg cggccaaggc cgctcttgcc agatctgaag ccaatgttat ttctataaga
    29761 aatagagttg aacggtacaa agaacttgtt gctatgaatg ctgtgagtca acaggaattt
    29821 gacgatgcaa cagccgccct aaaacaagcg gaggctgata ttcaggtgaa caaggcggcg
    29881 accgaaactg cccgtatcaa tctcaactat acacgggtta cagcacccat caccgggcgc
    29941 atcggtaaat ccaatgtaac ggttggggca cttgtaacgg caaatcaacc cagcccgctt
    30001 gcagtcatac aacagcttga ccccatctac gtggatgcga ctcaatcaag tgcaaacctt
    30061 ttagagttga agagacacat agcggccggt cggatcaaag gtgacgggcc taaccagaca
    30121 agggttagac tgattcttga agatggtaca ccttatcctc tggaaggtac tctgaaattc
    30181 tcagatgtca cggttgatgc aagtactgga tcattcatcc aacggatgat ctttccaaac
    30241 cctaaatata ctctccttcc cggcatgtat gcccgtgctc ttgttcagga aggagtggca
    30301 gaaaatgcta ttcttgtgcc acagcaggca gtttcccgcg atccaaaagg taatccgctg
    30361 gttctgacgg tagacgcatc cggcaaggtg gaacagcgaa tgataactgt ttcccgtgcc
    30421 atcggaaata aatggaatgt agcgtcaggc ctggaacagg gtgataaagt tatcgtggaa
    30481 ggtatacaaa gggtacgtcc agggatggcg gttaaggtcg taccttttga cagcactcag
    30541 aaggacagcg ctgagacagg gaaaaatgca aaccctcccg aaaaaacaac tccgccaccg
    30601 gaaaaagcaa agtagaggga gggttcacca atgctatcga aattctttct caaccgtcct
    30661 gtcttcgcct gggttatagc tataatcatc atgctggtgg gtgttcttgc catatacaac
    30721 ctgccaatct cacaatatcc acctatagca cctccttcca tttatatcgg aaccatgtac
    30781 ccaggcgcct cggctgagac tgtggaaaac agtgtaaccc agattatcga acagaaaatg
    30841 accggtctgg acaatatgct ttacctctct gcccagagtg attccgctgg aggctcacgt
    30901 atagagctga cctttgcccc ggggacagat cctgaccttg cctgggccaa ggtccagaac
    30961 aagcttcagc tcgctatggc aaatctgccc gaggttgttc agcaaagtgg tgtcactgtg
    31021 ggcaaagcaa ccagaaactg gctattaata gccaatctca tctcagaaga tggcagcatg
    31081 gatgggaatg atttacgaga ctacgctcag tctaatttag aaaaggtatt agccagggtg
    31141 ccaggggtgg gagaggtgga aatgttcggg tcccagtatg caatgcgcgt ctggttgaac
    31201 cctgacaagc tggttgatta cggtattact ataaacgatg ttattgcagc actccggatg
    31261 tataacgtag aggtctctgc cggccagttc ggtggcgctc cggctgtgaa gggccagcgc
    31321 ttaaacgctt caatcatcgt tcagagcatg ctcaaaaccc ctgatgagtt tgccacagtt
    31381 cccattcgta tcaaccctaa tggctctgtc gtaaagatta aagatgtggg acgagtggaa
    31441 cttggtactg acaattacga tattgaagcc ttttacaatg ataaaccctc tgctggcata
    31501 ggcatacgca tggcttcagg ggcaaacgca ctcgacaccg caaatgctgt taaggcgaaa
    31561 ctggacgaga tgagcaggtt cttccctcat ggtatgaaag ttgtttatcc ctatgatacg
    31621 accccgttta ttaaggttgc tatctgggaa gtggttaaga cccttttgga agcaattctg
    31681 cttgtttttc tcgtcatgtg gctattcatg ggaaatatca gggcaaccct tattcctact
    31741 gttgcagtac cggtggtgct cctgggtact tttggaacgc ttcatttctt cggtttttct
    31801 atcaatatgc tcactatgtt tgctatggtc ttgtccatcg gtcttctcgt tgatgacgcc
    31861 attgttgtgg tggaaaacgt ggaacgaatt atgaccgagg aagggctttc accaagggaa
    31921 gcaactgcca aatcgatgga gcagataaca acagccctta ttggcattgg ccttgtcctc
    31981 tccgcagtgt tcggtcccat ggctttcttt ggtggatcta caggagtcat ctatcgtcag
    32041 ttttcagtaa ctatcatctc ctccatgctc ctgtcggtaa ttgtggcatt gatcctcact
    32101 cctgtcctgt gtgccacgtt cctcaagcct gtaaaggcag gacatgaacc tgctgaaaat
    32161 tcgattcggt tcctccgccc tttctttgaa aggtttgacc gtgtcttttt ccgcatcagg
    32221 gaccgatacg taaaaatagt tggaaactct ttttcgagaa gaacgcgcta tgttgttata
    32281 tattttgtta tcgtggcagt ggtagggatt ttaatctggc gcattcccac atcctatgtt
    32341 ccagatgaag atcaaggcat gatgcttgcc cagatcatgt tgccatcagg ctccaccatt
    32401 gagcagaccc aggaggtcgt caatgcggta caaaagcact ttcgagacaa tgaaaaagaa
    32461 gcggtagatt cgatgatgac gatttccgga agcgggtttt caggaagagc acagaataat
    32521 ggcatggtgt ttgtgaggct gaaggattgg aatcttagaa accggtcaga cctcaaggtg
    32581 aaggctgtcg caggaagggc catggcggca ttctc
//
LOCUS       DAOOMC010000016        31675 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdus sp. isolate AMR_MDS_2685
            NODE_1201_length_31675_cov_5.900601, whole genome shotgun sequence.
ACCESSION   DAOOMC010000016 DAOOMC010000000
VERSION     DAOOMC010000016.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864760
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdus sp. (wastewater metagenome)
  ORGANISM  Syntrophorhabdus sp.
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae; Syntrophorhabdus.
REFERENCE   1  (bases 1 to 31675)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 31675)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,729
            Genes (coding)                    :: 2,708
            CDSs (with protein)               :: 2,708
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 7 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31675
                     /organism="Syntrophorhabdus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1201_length_31675_cov_5.900601"
                     /isolate="AMR_MDS_2685"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2699756"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..669
                     /locus_tag="PK125_02815"
     CDS             <1..669
                     /locus_tag="PK125_02815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB37072.1"
                     /translation="TGMESRAVGGLQRVIKVDRKIRVEQYTDHIRKTYASRHRLERAW
                     RNHVVLAYMNLSIAPTAFYVERPGDGSMGSITMENLQGKGQELDRYLDGKYDTMATHE
                     RRRFIARLTDFLLMITRKKIIHKDMKACNVFVFHEGGFMLLDVEDIRFRALDEESLKR
                     MLIQLNTTIPRRVSTGDRIRFFLQFTSPMKLDKKAIFSAVVRESMKREIVYEGVDRLK
                     REDW"
     gene            complement(753..2552)
                     /locus_tag="PK125_02820"
     CDS             complement(753..2552)
                     /locus_tag="PK125_02820"
                     /inference="COORDINATES: protein motif:HMM:NF039835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HPB37073.1"
                     /translation="MKQKLIRLYTFFILSLLCLICFTSVSAAATVNQYGIVFDNPYVI
                     DRFMHEGNWVDQVIVPGRPPPIYRARAAIIPEPQKTAGTNTLTNVPAFDWSYGCSATA
                     AAMIFGYYDNTGYPDIYTGPTNDGNMPMTNEAWGQGECPLSATRQEIDGRTTRGHVDD
                     YWVGYGSTAPDPFITHGWVEHTKSECTGDFMGTNQSQYDNFDGSTTFFLYTDGSPTYD
                     FTLYEPDKRDGCHGMRLFLESRGYTASSNYSQYILGYSSNIRGFTFEDYKQEIDAGRP
                     VMIQVAGHSMVGYGYNDTGTLVYLHDTWDYEPHEMTWGGNYEGMQHWGVTVLVPSTAP
                     PGTSTLRVTISGNGVGTVISSPSGIDCGTDCSGVFEHGTLVTLTAQPDAHYLFTGWSG
                     ACAGTNPECVIIVNEDQAVNATFEKPLSVNEGSIGTVLTINGTGFGTRKGKVLAGGIA
                     AKVAKGDWTDTRITCTITKPPLPAEMAYPVAVVVNNVSRHLDDTFTVRNPVLDDLLVS
                     RGRFPDVLTVTGKFFGSKKGKVYLEDLYGKKKNLKVTFWEMIPSTGISTLMFRVPRPS
                     KKFPAGYPYVLKVAGKIGTATANTGFVIEGPLP"
     gene            complement(2552..2836)
                     /locus_tag="PK125_02825"
     CDS             complement(2552..2836)
                     /locus_tag="PK125_02825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB37074.1"
                     /translation="MGMEKVVVIESTVPAPPAGRYYEGTRIMPFLESAAGSRMYSPLG
                     SGYHDAYPGVFQITPGGVNPERYRFAGDVLEHAASEKRFVDFIISLPGDQ"
     gene            complement(3228..4241)
                     /locus_tag="PK125_02830"
     CDS             complement(3228..4241)
                     /locus_tag="PK125_02830"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HPB37075.1"
                     /translation="MIIPIFLPHLGCQSRCIYCDQDVITDIEKDDIGTRIDESLAGRK
                     GPLEVGLYGGNIFGLNSEDVKRLFSYFRSHEDKITNFRVSTKPVPLDPEVIAILRENR
                     TTVIELGIPAFNDHILWFLNRRHTTADLINAFSMLTGEGFQVALQVMVGLPHETLDDV
                     RGTVRNIVKLKPSYIRIYPLAFISGTPLAAMHEAGAFTAITFEETLQRTVLIYLNALQ
                     HGIKTVKMGLTDNEVIKDRIVGGYYHPAFGFLVKSQAFHQALMARVIEGGFTGYATVC
                     LNSRDISHFLGYKRTNLARFREAGLSVTWEAANIAPGTFILKTGKKKIWGTIFDALSE
                     LNK"
     gene            complement(4384..5055)
                     /gene="rnc"
                     /locus_tag="PK125_02835"
     CDS             complement(4384..5055)
                     /gene="rnc"
                     /locus_tag="PK125_02835"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR02191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HPB37076.1"
                     /translation="MRPTPLEKTLDYTFKNKDLLHQAITHSSCFNEKKATRHSDNEKL
                     EYLGDAILNSVITILLYKKYKDKDEGFLSNARSCLVKRETLTEAAKSIDLSKHMSYGF
                     GEQNVPQESKVLSNMMEALIGAIYLDGGMRRVTRVIKQLFSPFFTEEKLTEKNPKNLL
                     QEYSQKKWGILPRYKFSRKTRSGFAVLVYIGKDQKAKGSGKSKKEAEQNAARELLQQL
                     EGGDA"
     gene            complement(5139..6719)
                     /locus_tag="PK125_02840"
     CDS             complement(5139..6719)
                     /locus_tag="PK125_02840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HPB37077.1"
                     /translation="MADIYIHIGEVLARNARKFPNDIALVEKVPAEKKRTEITWKQFD
                     DQANQFSNVLASKGVKKGDKVLHFMMNSIDWLVAYFGVVRTGAWVVPLNFRFTAEDVK
                     YCSEVAEPMVVVYDEEFAPKIDAIKDQLPTVKSFICLGEKIPAYAESFKKLMETAPAK
                     SLEVELTFDDTCGLYFTSGTTGQPKPILLTHKNMMCACITENAHHWQRKKDVFILIPP
                     LYHTGAKMHWFGSFIVGGKAVILKGVSPEWTLEAVSEEGGTIVWLLVPWAQDILLKFD
                     SGELKPENYKLDQWRLMHIGAQPVPPSLIKHWKTYFPNMQYDTNFGLSESTGPGCVHL
                     GIGNEHKIGAIGIPGFNWEARIVDENGNDVKRGDPGELIVRGDGVMREYYKNPEATAK
                     TLIKGWLFTGDMARIDEDGFIWLVDRKKDVIITGGENVFPVEVEDFLHTNPNVKDAAA
                     IGLPDERLGEIVAVIIDVAPGKSLTADEVLKFCEGLPKYKRPRKVFFGEVPRNPTGKI
                     EKPKLRKMYSGMEEAFKI"
     gene            6875..8467
                     /locus_tag="PK125_02845"
     CDS             6875..8467
                     /locus_tag="PK125_02845"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate-forming enzyme family protein"
                     /protein_id="HPB37078.1"
                     /translation="MNFGMFSGLNASKFPKREFIIESYAEKGIRRSLTWEQFDDQANK
                     FANYLMKECGVKKGDIVLHLMMNCMEWYASYIAVLKTGATVTPLNFRFASADIKYAAD
                     VTKCKAFLFGDQFNARVEPIMKDMDYCKNFICLGQTPAGVKSYEEIMQKGDGSSVCVD
                     VDDNDMAELMFTSGTTGAPKPVSHTHKTLFFIGLGNALTYNEGYSSIYLSPHPFYHSG
                     TLFLSFPCYIAAGKILMPMDLKPEFYLKSMADEKCTGGWNTVPTWSDVISAIKTGQVD
                     IKKYDLSALRHIEIGAQPVPYVLLEDSKKIFPNLPIANIYGITEGGGGGTLNCYDEDI
                     MRKPGSIGKATVFMEAKIVDSAGNRLTNGAVGELLLKGPRLMKEYAFNPEKTAETIKD
                     GWLYTGDLAYEDEEGFIFFADRAKDLIIRGGENIFPAEIEDTLRKHPKIQDVAVLGYP
                     HPRLVEIVMAIVQPKEGETITDEEIINFVKDKGLAKYKWPEKMVYTTIPRNPAGKIEK
                     PKLRDMYVKPAKEAMEKEFKKQ"
     gene            complement(8557..10188)
                     /locus_tag="PK125_02850"
     CDS             complement(8557..10188)
                     /locus_tag="PK125_02850"
                     /inference="COORDINATES: protein motif:HMM:TIGR00704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005436 - sodium:phosphate symporter
                     activity [Evidence IEA];
                     GO_process: GO:0044341 - sodium-dependent phosphate
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HPB37079.1"
                     /translation="MAECRTTSIEKMINSLFLFITGLTIFLFGMIKLSTLMRGLFSGR
                     IRGYIRMSVRRPIYGLFLGIVSTILFQSSLATTLVTVGIVSAGLVSFYHSLGIILGAD
                     IGTTLTAQLVVWKVTSISPLIIFAGGFLFFAGKEQWKTIGEAVFYFGIIFFGLSLISD
                     ATSPLKENEAFLHFFRNTKNPFIGLAIGLIFTAIVHSSAIPVSTLIILGQQGLISIEN
                     ALPIVLGANIGTTVTAIMEATVTNVNGKRTAIAHLMFKVFGVVLCLALFPFFVLSVKK
                     MSGSVAQQIAYSHFLLNLTIAMIFIFILKPFSRLLEAIIPGTDQVLPLWPEFLDEKCL
                     VSPEEALACVNKELGREIALTQKMLITSISLIDKFKPAKQRDLAYIELVVNNVQTEIT
                     QYLWNLSCNHLTPALSKRLFAFSSLVYDIERIGDRSTNIAELAESRHKRKAQFSEPAK
                     KELDEISKLVVRNLDDAAMLIEQRDTKRIQDVFERHLEVLLAIKAATQKHLERFYQKV
                     CLAEAGPIFVDVLVNLERISDHCQVIAEHISNVDE"
     gene            complement(10161..10466)
                     /locus_tag="PK125_02855"
     CDS             complement(10161..10466)
                     /locus_tag="PK125_02855"
                     /inference="COORDINATES: protein motif:HMM:NF017451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HPB37080.1"
                     /translation="MFEFKCIDSFDFEKLTLYQMALDFNDRINITGQFPPEEKFSLVD
                     QYRRASAYFPLNIVDISFRQRYLEKDVRDQGRSGYADPSRMIHYLLKSVWQNAEQRA"
     gene            complement(10469..10798)
                     /gene="metG"
                     /locus_tag="PK125_02860"
     CDS             complement(10469..10798)
                     /gene="metG"
                     /locus_tag="PK125_02860"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR00399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase subunit beta"
                     /protein_id="HPB37081.1"
                     /translation="MDNITFDDFMKLDLRVAEIKACEDVPGADKLYKLTIDMGQERTI
                     VAGIKLHYTKEELLGKKIAVVANLEPRKLRGIESHGMLLAASSEDRSSVVLLTLDRDI
                     TPGAKIS"
     gene            complement(10919..11533)
                     /locus_tag="PK125_02865"
     CDS             complement(10919..11533)
                     /locus_tag="PK125_02865"
                     /inference="COORDINATES: protein motif:HMM:NF013706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HPB37082.1"
                     /translation="MFHPTKIIAIGLNYLDHAREFNMELPAYPLIFMKPQTAVIGNAE
                     TIIYPPQTKELHYEGELAIVIGKKTRNVAVENAEHYIAGYTCANDITARDLQRMDGQW
                     TRSKSFDTFCPLGPKIAKNIDPTNLAIATRVNGITKQKSNTNQMIFNVFQLVSFISGI
                     MTLMPGDIIITGTPSGVGELQAGDTVEVEIEEIGILKNTVMRLA"
     gene            complement(11693..13585)
                     /locus_tag="PK125_02870"
     CDS             complement(11693..13585)
                     /locus_tag="PK125_02870"
                     /inference="COORDINATES: protein motif:HMM:NF024779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4105 domain-containing protein"
                     /protein_id="HPB37083.1"
                     /translation="MFRISLFILCLISFFIIFSKVSAGNPTYDLALIEEADRRALYND
                     KYWHTLLHYKKGVFGLRSLIDDPNFFLAPGGKHNPKAELEATIKAFFQEPGEGTKHPV
                     CRFVARYTWLKEQLGIDPSQLPVPECTHFEQLMVEIKPEAVSLIFPTSHINSPASMFG
                     HTLLTIETATQSKLLSHAINYSAITTDTFGPLFAVKGLFGLYKGYFSILPYYGKLQEY
                     SDIDHRDIWEYPLNFTKEETRRLLMHVYELDSIYSDYYFFDENCSYDLLFLLDAGRPS
                     LNITDQMDLWVIPIDTIRLAKKNGLTNKAIYRPSKTTKVQYVASLLSQKDRESARAMA
                     NGEIQPKDILVQAISQDEKATTCDLAIEYLQYLYTKKRISKHQYLDRFLELLKIRSTL
                     GQSTEDRYAIPQPPRPDEGHESNRLSLGTGIKKDRFFYELRYRPAYHSLLDNDKGYPE
                     GSQIVFGDTTLRYYPSLNRLRLESLDIIDITSLTPRGLFFQPLSWKIKTGLTHRYMED
                     GREHMVYQLNPGGGFAYKHNATGLWYAMAETALNVSGAMKEMYALGAGASVGLIAKVT
                     DLFKFHVCARNIYYGIGDYHNYFEAMVQANYTVSSNTGLAFQISRTKTRSFYQTEGAL
                     SWNIFF"
     gene            complement(13634..14104)
                     /locus_tag="PK125_02875"
     CDS             complement(13634..14104)
                     /locus_tag="PK125_02875"
                     /inference="COORDINATES: protein motif:HMM:NF022666.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3015 family protein"
                     /protein_id="HPB37084.1"
                     /translation="MMKKVVTVSLVALAVICLGTAVWADQKNYGCGLGSIAFEGNDGL
                     ISQVSAATTNGTFGNQTFGITSGTSNCEQFKTFASNEKINTFVAENMDSLAKDIARGH
                     GEYLDTFAGLIAVPESQKTAIYASLQANFSKIYTSDSVSYLDVLRNIDSVIASS"
     gene            complement(14197..14511)
                     /locus_tag="PK125_02880"
     CDS             complement(14197..14511)
                     /locus_tag="PK125_02880"
                     /inference="COORDINATES: protein motif:HMM:TIGR00106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MTH1187 family thiamine-binding protein"
                     /protein_id="HPB37085.1"
                     /translation="MSVLLEFAMFPTDKGESVSEYVGRILKVIDEEGHPYTLTAMGTI
                     IEVQSLDDALKIVEKAYRQLEKDCNRVYSTIKLDIRKGSAGRLKQKIVSVEKKVGKKL
                     ST"
     gene            14676..16319
                     /locus_tag="PK125_02885"
     CDS             14676..16319
                     /locus_tag="PK125_02885"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HPB37086.1"
                     /translation="MRVLSDSEQIYLDRIDALQEKIHDLEKRLERFERVEEALRESEE
                     RYRSVIDNVEVGIALISPAMEIISLNNQMRKWFPLIHVDTRPICFTAFNDPPRDSVCP
                     YCPTLKTLLDGKVHQSVTRTPSGGKIRHFKIIASPLRDKKGEVVAAIEMVDDITRRKH
                     AETLLRKEKRVFFSVLQKAPYGAVIIDKRGKYLFINEEFTKITGYDIADVPDGVSWFL
                     KAYPDPESRKEVLKTWKEDITEKGVSRVFSIECRNGEKKDIEFRSTKLSDDRYLTMLS
                     DVTEQKRAEMELKQARDELEARVASRTAELFKLNCDLQDNIALLKSINAELKAFSYSI
                     SHDLKTPTIAVEGFSQILLERYGHRLDKKGKNFVRMIRESAGQMRELIDNLLAYSALG
                     RKKLKLSMIDMEGMVIEIFNQFKAAYRGHPMSISIKPAPPANADKTMIRQVMLNLLGN
                     AIKYSRVKEQSDVKFGGWSEPGRVVYYVQDNGVGFPMEQSKRLFEVFERLHPPEEFEG
                     AGIGLATVKRIVRRHGGEVWAQSTINEGSTFYFSIPILS"
     gene            16878..17669
                     /locus_tag="PK125_02890"
     CDS             16878..17669
                     /locus_tag="PK125_02890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB37087.1"
                     /translation="MRKSAKCTIIISAIIGVLLGLYLVFGYIRTPVIHGTVLDAETKQ
                     PVENAWVTGTLSLKVATIQGDIHVHPAFAPAHLRTNKEGKFIIPRKSFRQPIPPLGFG
                     MNVEGCRVTVETINDKQGEISLKPSFWKWWTEVTIYVKPTLMTEGEYDSYLQSLFRYC
                     TTGRSGVEVPVAKEGCDAWELDYAIAKHEYFLKNLGQLDSSDKRILYSGTLHNLAYLY
                     KEKGDYRKALHAFTVGRDFDMKRNIDLWIREYNSQIEELKKILQK"
     gene            17692..28404
                     /locus_tag="PK125_02895"
     CDS             17692..28404
                     /locus_tag="PK125_02895"
                     /inference="COORDINATES: protein motif:HMM:NF019325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP-like repeat-containing protein"
                     /protein_id="HPB37088.1"
                     /translation="MQKKLPTIILLFVVVSWLVPLVHGFDNASKAPQQVKVEYTSQMA
                     GNFLGGHDTITAEGLLLKKQVHYGDDRFKQWADRDAASPLRIGAHDEDTNQLLNFILR
                     DPPLGPNGWGDFYSHFYDSRIGDDAENAIGRQATKAVKDYLEKIKELTNCTSKGFETL
                     SPGDKTRVYDYLGRITHLYQDMFCPSHVKVEAHPFHHPFEQYINDKWGEIIRSSSFQD
                     RVTTSAYLKGNYDIGSSINPIQAMKATAEKSRAFWGEEALCDLWDEQKSSCLLINSEK
                     LQQNVDFLVPEAVLSTAGYIDSIYRAAKATPDYRNICLPKPLQGPGGDHPDDRFDVSD
                     AFYWKDRFNLSEMKLNDLFMRTGLKKGKTGVWYGKRLKELYGKGRGAPSDMPISEKTK
                     IYNEFKTVHQKLNEKSSSKDWLTSPDIALFAYGFYNEAISLMIKINEPARFLDLDFDP
                     AIVQDHPVLLIPTAGLAGYEQSATLKAKLEEYVRLGGTLVVFAQQLGHHWNILPTPVD
                     LATGEAKSVSGYGYQQDQNCQQGSVFIENNHAMLSSFASATIDVGVDGYLCSYPDNST
                     ILLRRSANGQPAMILYPYGNGHVIVTALYTDFAFSRGQANKYEIDLVANIISWAKKPA
                     ELPQVKPGETISLNIPVTNNMDSNASSVKLRIYNPTRSLLLSEQIIAGSVPASESTIF
                     VTQFTIPGNGEPGIYRADYVLLDTQGDIIQPQTENDFSRFVVNKPSTTGSPDKPIWFS
                     VTTTSQNVPFGATFDYTFHVYNNSDEIRHLTIKSIFGHTKRPHEWHVTVRPKAVTTIT
                     GSDIFEDRAYMYDTMRAFLYDELNQMIGRYELSFKGFYPHAYIKLSSNKQVYSNNEDV
                     ALKVVTTTGQTGAYTIKTTILGPDNTLQFEDNRTLNLSSGSNTIIDIHHHLAVNSNPG
                     SYIARSEIWKESRFLSSASTSFELPRSQIVITFNEPQSFKVGANLLSFGLRNNGKIDI
                     RSGTFTITLTDPHSTVIYEGSHSFSLTSGKSSTLTIPISIPNLYFGDYIITYLQSDET
                     KNGSVVSLHIPNSATIGLLLDKPSYRIRESANISATIINTGKFDLDNLQATLSVYSLA
                     FQNTKIANVAKGQNVALSFSIPIPENAWAGSHFAELYLAAPSGDSMQQTTVVSIPDSS
                     LKVHFNGPNTLNPGDNIALSIENIGGVDAEYIAEKLSLTDNNGTAIHNGTANGIVRVG
                     EVKSFYEFQIPNQVVRGDVMLNVAVKDNNTGLITNFLKSFLLSGLEARIETKTDKNLY
                     AIGESTMNTTDIINLGSHIENANLDLLVYKLKAGVEGQFSHVLPKGWLPLIQPTDIAS
                     DSEGYLYVVDVEDRRLVKIQKNGSFVKEFNYMNRCLGGGREVCQPYSVAASPDGFIYV
                     VDKGSSWYLNIHNCIHKFDREGNFLDSWPYCNSGPGFSLDQPSGIAVGRDGSVYVTDT
                     YNHRVVKFSSEGTFISAWGSRGKEPGKFVYPEGIATDSSGNVYVTDTEGYGYNSLCSH
                     RVQKFDMDGNFITQWGGYGTKDGLFMNPLGIAVDANGYVYVSDSSQSVNKSYRIQKFD
                     SNGVFVKSWGGEGRIEGKFVNPKGLAVDEDGYVYVADSGNGRIQKFSADGDFISLWGG
                     RGLTNGFLAGPESIGVDAQDNIYVADHSNYRIQKYDKNGNYIKQWGSIGTGDGQFFCG
                     PKSIAVSKEGFIYVPTCDRKIQKFNSEGDFQTSWGEKGTDDGQFSGLLRIAVSTGGYV
                     YVSDDEAKRIQKFDSNGTFIAKWGNRGSGPGQINEIRGISVSPDGMVYVADAHRIQKF
                     GEDGIFLLEWGAEGTNAGQFKSIARITVDSMGFVYTVELNPIRIQKFDGAGNFITQWV
                     EGEGDIVDAFRSVSGIAIDSDGSILISDSYRHRICRLTPLGTDVVFQKSFSLSQPGNT
                     NQQYNNTIGAITRCGTHYCEATLKNSLGQTISKSFYPFYVASGDVAILMSIEKKNYKP
                     GEQIRISGEVVNMSDTPLSGYNLNITKLSSLGVNELFLNDFDLPARGIFPFSFTFSEL
                     REGKYVLTATVQNASLKVADASEHYEVHIPTLSTEIISPDIASDQPFSLDVVMRNTGD
                     VDANVNLSSSINEETFSLSMAPGSTRVITYSQHISEDTLYRFTISGDVNETLEKMVYQ
                     GLATSVYVYAANVYPEGKVAIPVSVENPGLLDGKISLDIDLQPTGYSFQKNYFVPRWS
                     SASDNLIFDLAEGEYTLTLTSQAPPASTTASFSVRRENNVTMAPLVIGTLTNGFVAVS
                     SEFVNHGYRDVNGTIQLSLLNAQGGVEWESSNEVNLPASLSPTPVPANLSFPSSIIQV
                     GNYTVKVVLLNETQQELASQTAPIVISGASLHLVQVPSIQTVPAGEEAMFTFVVANTG
                     GKEAQAELTLRSHDFINLTQRVTVSPGEQKNIQFAFPIPADLEEKDYYAEYELKDRDS
                     TSVKGQVKYHVTGINISVAASLDKEHYREGETAHLALIVSEQSGGGTRNLFARVNYGD
                     FESKQNFTLTGNQNLSFTVPLTAITGEKLFFGIYDEGGRSIHLNSLYIYKAGDALTIT
                     TNKQVYNLGETVNMTISGTGIGTMTISGPGGYSETFLFSGSVARNFVLPTFATAGTYY
                     IEASLVTAEGQTVHITRPIDIAGISVKVKEASLDKGKYLPGEPLRLSMRIESNRNVAA
                     TLKVWSVDPDKTSIFLGEHQLSLTREEQLLLTTDYPTTITTSGIHKIIYGIYADNLLL
                     SSGNLSFDAGGGVILGMMTDRIEYPLGSETVAVKFNILGTTDATLTLKLNGIAVHSEA
                     INLNGFTSVSLPLPDPGPGAHMVEGILSTDGLSSRKETRFLYGTNLPDLAADVWSSGT
                     WIGKDGTLKLFATVGNRGKTTTSSTSMTLHDGGTILAAFSVGELVAGASQSFEFLWNV
                     MGKAGEHTLVAAIDPTNSITDFARDNNQVSRRIVIPDIALVTEIDKDNYRMGEQVTIN
                     STGINLTAGTNYPDLVCTTVVRDLSGNEVFRQSKPFGLSPSQIGVATFTWSTSSVTTE
                     GKYTIKQDITSGATVLAERTRTVTIAARTGFDLRVDPASIRLKQGETGLFTVSVDAPS
                     GWNNNVSFDVEGAPAGAIISFTPDAVTGPGTATLSIVTSAATPHGTYSIVVRGRNGEG
                     SGEVVQATPVTIDISGFSLEAVPESLSISQQETAEFLVSAVSLNNYTGNVVLSDATGE
                     IEGMIITVDSPTLTVPGGQAIVRVQSSKSLHPGAYTIKIIGSDGIITRDIKLSVHVTQ
                     STDTIPGFVLTPGPGPTNRALVTLLNSNFEEKMEFTAFKTRFGANAVMGDIDGDGVDE
                     IVVAPGPDPLADGGVRVFRQNGTLLFEQPVLATKFGAYLAAGDIDSDQREEIAVGAGP
                     GLSNTARVKVLSYNGHKFENTGIDFIAFPKTYKLGVKVALGDVDGDGILEIIAGAGPS
                     PLSPARVKVFKVDTSGGVGNWSVSSTLSDFIVEFGDWYPYIFGVNVAAGDVDGDGMAE
                     IIAGAGPNPLQKAVVAVYRGDGTFTGTRFDAYPSNEYRFGVNVAARDLDGDGLAEIIT
                     GPGPSPFSDSWIRIFRGDGTLLSDGFLAYPESMKFGSKVSIGTVGE"
     gene            28522..28598
                     /locus_tag="PK125_02900"
     tRNA            28522..28598
                     /locus_tag="PK125_02900"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:28556..28558,aa:Met,seq:cat)
     gene            complement(28715..29371)
                     /locus_tag="PK125_02905"
     CDS             complement(28715..29371)
                     /locus_tag="PK125_02905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB37089.1"
                     /translation="METRKIYLVLLCGLILCTFFERLDAREAMVDPVKDAKIKQLANL
                     SAENVFQNLKKEDFFYDDEFLKRGIDRAFKNRKNEAVRFAISHLKTGKKTRDIEEAKK
                     FHVAKKILQMFPDESQGYLAELYSDGNPGMRRNVVHAVAGMPASDFARYILMNALEDK
                     AVCEEKSSDTVGEPLRVCEVAYNLIVLHYQVENVLRTIGTVHRIEVRDFHIERLKAIF
                     "
     gene            complement(29397..>31675)
                     /locus_tag="PK125_02910"
     CDS             complement(29397..>31675)
                     /locus_tag="PK125_02910"
                     /inference="COORDINATES: protein motif:HMM:NF013955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HPB37090.1"
                     /translation="TISLRVRDNNNAWSAPATDSLIVSSGGSEYQLTLTSTYSTDVNS
                     SGTKYYHDGADWVGRGNSGALRAVNRWNISGIDPSWTITSVEVRFYTESTAGSPGTLF
                     LTRYGTSHGEDNPQADTGAQVYSKINNTPYASLPEPFSGSWTPWINLGPAAVADIAWC
                     RMGGLGTWSAGLKASDAIESGTTIRHADFSEDNETNNAELRVTYIGGSSSNTPPTATI
                     TSISPNPATAGQTVTFTGSGTDPDGTITAYEWTSSINGLLSTASTFSTSSLLPGTHTI
                     SLRVRDNNNAWSAPATRNLTINATSSGTTEHIYICLGYRLPWLADKKAGLEYLVRTMG
                     AQKDGDVWRYTNKTIKKEFLIHFVEDIDSMKLALQQEEAHILFLGHSNYGLGPVFGTA
                     DEIRNEVIEDIYWIDDPKILTISSPWVAPAVRAIRTNHSFPDWWTDFQDGTSGIMPYD
                     FSDPSIDPPYNYYITYRIPGDPNLYKVETTRNGAIERFPDSNIPAWYSPDGRSPDPTN
                     PDELRYFIVKTPTAPSLNVIGEWTWSKEVTGFYRENYFYSSAGTGQNRVEWLFTIQEP
                     GYYKIFGWWPAASHHTKGARYTVNHTTGSNIFIVDQTKNGGQWNEFGQFYFEPGNYSV
                     VLSDQASSGRVIADGVRITSVVAGVLNKQIDNMVYPSPHFEYKTVVSRKDLSVQKENM
                     RYSRLFYDSCNSGNYFLDTLNRGIAFFTVNDSSHLGFDAYIKAYLEGKSNREIWTIIQ
                     GYEAVYDYYDFNKLPSQQ"
BASE COUNT         8770 a         6889 c         7284 g         8732 t
ORIGIN      
        1 acgggtatgg aaagcagggc cgtaggaggg ttacaaaggg ttatcaaggt cgaccgcaaa
       61 atacgcgtgg agcagtatac cgatcatatc cggaaaacct atgccagtag acacagactt
      121 gaaagggcat ggagaaacca tgtggtcctc gcctacatga atctcagcat tgccccgacc
      181 gcattctacg tggagcgtcc cggtgatggt tcgatggggt ctatcactat ggagaatttg
      241 cagggaaaag gccaggagct cgaccgctac ctcgatggaa aatacgatac catggctacg
      301 cacgagagaa ggcgctttat tgcaagactt actgacttcc tactgatgat tacccgaaaa
      361 aagattattc acaaagacat gaaggcatgt aatgtcttcg tcttccatga aggggggttt
      421 atgttacttg atgtggagga tatccgattc agagctcttg atgaggagtc tttgaaaagg
      481 atgttgatcc aattgaatac caccataccc aggcgtgtca gtaccggtga tagaatccgg
      541 ttttttcttc aattcacttc ccccatgaag cttgacaaaa aagccatatt cagcgcagtg
      601 gtgagagaat cgatgaagag agagatagta tacgaagggg tggacaggct gaaaagggaa
      661 gattggtaac ggactgttta taaaatgaaa gcttcgacag gggagtaaaa ggggcaggag
      721 gttataaaat aaaacccact gtcgtctgca gtttacggta gaggcccttc tattacaaag
      781 cctgtgtttg ctgttgccgt ccctatcttg cccgcgacct tcaggacgta cgggtaaccc
      841 gcaggaaatt tctttgaagg cctcggtact ctgaacatca acgtgcttat tccagtggat
      901 ggaatcatct cccagaaggt gactttcagg ttcttctttt ttccatacag gtcttcaagg
      961 tacaccttgc cttttttgct accgaagaac tttcctgtta cggtaagcac atccgggaat
     1021 cttcccctgc tgaccaaaag atcatcgagc acagggttcc ttacagtgaa ggtgtcatca
     1081 aggtggcgag atacattgtt gacaacgacc gccacgggat aagccatctc tgccgggagt
     1141 gggggttttg tgatagtgca tgtgattctg gtatctgtcc agtcgccttt cgcaactttt
     1201 gcggcaatgc cccctgcgag aacctttcct tttctggttc caaatcctgt tccgttgata
     1261 gtgagtacag tgcctatgga gccctcattt acagaaagcg gtttttcaaa tgtggcattt
     1321 actgcctgat cttcatttac gattataacg cattctggat ttgtgcccgc gcatgcgcct
     1381 gaccagccgg tgaataaata gtgtgcgtct ggctgggctg tcaaggttac cagagttccg
     1441 tgttcgaata ccccggaaca gtcggttccg cagtcaatgc cggatggtga tgaaatgaca
     1501 gttcccacac cgttgccaga gatagtgact ctgagggtgg aagtacctgg cggggctgtg
     1561 gatggcacaa gaactgtgac gccccagtgt tgcattcctt catagtttcc gccccaggtc
     1621 atttcatgag gctcataatc ccaggtatcg tgaagataaa ccagtgttcc cgtgtcattg
     1681 tacccgtatc cgaccatgct gtggcctgcc acctggatca taaccggcct tccggcatcg
     1741 atctcctgct tgtaatcctc aaaggtaaac ccacggatat tgcttgaata tcccagtata
     1801 tattgagaat aattactgct tgctgtgtat cccctgctct cgagaaaaag tctcatgccg
     1861 tgacatccgt ctcgcttgtc aggctcatac agcgtaaagt catacgtggg gctgccatca
     1921 gtgtacagaa aaaacgtggt tgaaccgtca aaattatcgt attgagactg attggtgccc
     1981 atgaagtcac ctgtgcactc gctcttagtg tgttcgaccc aaccatgggt aataaacgga
     2041 tcaggtgcag tgcttccgta gccaacccag taatcatcca catggcctct tgttgtccgc
     2101 ccgtctatct cctggcgtgt tgcactcaga ggacattctc cttgccccca ggcttcattg
     2161 gtcatgggca tattaccgtc gttggtcggc ccggtgtaga tatcaggata tccagtgttg
     2221 tcgtaataac cgaatatcat ggctgcggca gtggcagagc agccataact ccagtcaaag
     2281 gcaggaacat tggtaagtgt gtttgtaccc gcagttttct gtggttccgg gattatggcc
     2341 gcccgtgccc tataaattgg aggaggacgg cccggaacga tgacctgatc aacccaattg
     2401 ccctcgtgca tgaaccggtc gataacatag gggttgtcaa agacaattcc atactgattc
     2461 accgtggcgg cagccgatac agaagtaaag caaataaggc acagcaggga aagaatgaag
     2521 aaagtgtata accggatgag cttctgtttc attattggtc acctgggaga gaaatgataa
     2581 aatctacaaa cctcttttct gatgcggcat gttctaatac atcaccggca aaacggtacc
     2641 gttcaggatt aaccccgcca ggtgttattt ggaagacccc agggtaggca tcgtgatagc
     2701 ccgaccccaa tgggctatac attcttgaac ctgctgctga ttcaaggaac ggcataatcc
     2761 tggttccctc ataatacctg cccgcaggcg gggcagggac ggtactctcg attaccacga
     2821 ctttctccat gcccatcgtt ccctccacca gcagagctgt tagaacctcc ccgtcatgct
     2881 catggcattc gctgtgtagc ttcccgcgaa agacgcaagc agcacaggag gtcagtgttt
     2941 tcaaaggcag gtgaatccat ttatagattc atctgcagca agtatatttt agtgccgcgg
     3001 ccttgtccgt gtgagtctac agctaaagca acacaaaaaa taatacaagt caatgagaat
     3061 acaatttgcc ttcccgaaaa tactaatttt ccaattatat caatttaatt gaaacaatac
     3121 accctctttt tgaataacca tgtaatcaat gtattctagc gaaatatccg cataaactta
     3181 cttctacctc tcacctgctg caggagcctg tgccttctag tgggaaccta cttgttcagt
     3241 tcggataggg catcaaaaat ggtcccccat atcttttttt ttcctgtttt caggataaac
     3301 gttccaggag caatatttgc agcctcccac gtaacagata agccggcttc acggaatcgg
     3361 gcaaggtttg tacgtttgta tccaagaaaa tgggagatat cgcgactgtt gagacaaaca
     3421 gtggcatatc cagtgaaccc cccctcaatg actctggcca ttagggcctg atggaaagcc
     3481 tgactcttta cgagaaatcc gaaggctgga tggtaatagc ctcccacaat cctgtccttt
     3541 attacttcgt tgtccgtgag acccatcttc actgttttta tcccatgctg caatgcatta
     3601 agataaatca acactgtccg ttgaagggtt tcctcaaagg taattgcggt aaatgcccca
     3661 gcctcatgca tggcagccag gggtgtccca cttataaatg cgagcgggta gatccttata
     3721 tatgaaggct tcagttttac tatatttctg acagtccctc tgacgtcatc caatgtctca
     3781 tggggaaggc caaccataac ctgtaaggca acctgaaatc cttcccctgt gagcatgctg
     3841 aatgcattta tcagatcagc agtagtatgc cttcggttca gaaaccataa tatgtgatca
     3901 ttgaatgcag gtatgccaag ttcgatgact gtggtcctgt tctctctgag aatggcaatg
     3961 acctcagggt ccagaggaac gggctttgtt gaaactctga aatttgtgat tttatcctca
     4021 tgactgcgga aatacgaaaa taaacgtttc acgtcttctg aattcaaccc gaagatattt
     4081 ccaccgtaga gacccacttc taatggacct tttctcccgg caagagattc atcgatcctc
     4141 gtccctatat catctttttc aatgtctgtt atgacatcct gatcgcaata gatacaacgg
     4201 ctctgacagc cgagatgggg gaggaagata gggatgatca tttgaaatta tgggccatag
     4261 gtaatgggca acaggtgatg agcacagtag atgcctgatg cccattaccg aatattttaa
     4321 gcattcctct gcgctccatg tgctcaactc cgtgcttttt tcttgccgat caccgattac
     4381 gcgttacgcg tcaccgcctt ccagttgttg gagcagttcc cgtgcagcat tttgttctgc
     4441 ttccttcttg cttttaccgc ttccttttgc tttttgatcc ttcccgatat agacaaggac
     4501 agcaaaaccg ctcctggttt ttcgagagaa cttatatctt ggaagtattc cccatttttt
     4561 ctgggaatac tcctggagaa ggttttttgg gtttttctca gtcaattttt cctcggtaaa
     4621 gaaaggagaa aagagctgct tgataaccct ggtaaccctt ctcatacctc catcaagata
     4681 gatagcgcct atgagagctt ccatcatgtt tgaaagaacc tttgattcct gtggcacatt
     4741 ctgttctccg aaaccgtatg acatatgctt tgaaaggtct atggattttg ctgcctcggt
     4801 taaggtttcc cgtttaacaa gacaggaccg ggcattgctc aggaaccctt cgtctttgtc
     4861 tttatatttt ttatagagca gaatggtaat aacgctgtta agaattgcgt caccaaggta
     4921 ttcgagtttt tcgttgtcag aatggcgggt tgcctttttt tcattaaagc acgagctgtg
     4981 ggtaatggcc tgatggagga ggtctttatt cttaaatgtg tagtcgaggg ttttttccag
     5041 cggggtagga cgcatatctt gccgatggtg taagaaaagg gacgacaggt tcagccagtt
     5101 tcgctgttcc catcgccccc ttttctacag cctactactt atattttaaa cgcttcttcc
     5161 attccagaat acatctttct tagttttggt ttctcgattt tcccggtcgg attccgtggt
     5221 acctctccaa agaagacctt ccgcggtcgt ttgtacttgg gcaggccttc acagaattta
     5281 aggacctcgt ctgctgtgag gctcttgccg ggagctacat cgatgataac tgcaacaatc
     5341 tctccgaggc gttcgtcagg cagaccgata gctgctgcat ccttaacatt agggttcgta
     5401 tggagaaaat cctcaacctc cacagggaag acattctcac cacctgtgat gataacatcc
     5461 tttttcctgt ccacaagcca gatgaagcca tcttcatcta ttcgcgccat gtcaccggta
     5521 aacaaccacc ctttaatgag tgtttttgct gttgcctcag ggttcttgta atattctctc
     5581 ataacaccat caccgcggac gatcagttcc cctggatcgc cccttttaac atcattccca
     5641 ttctcatcaa caatccttgc ctcccagtta aagccaggta tgccgatagc accgatcttg
     5701 tgttcattgc cgatgcctaa atggacgcag cctggtccgg ttgattcgct gagaccaaag
     5761 ttagtgtcat actgcatatt cgggaagtat gttttccagt gtttgataag actcgggggg
     5821 acaggttgag cgccgatatg catgagcctc cactggtcaa gtttatagtt ttccggtttg
     5881 agctcgccac tgtcgaactt caaaaggata tcttgagccc agggcacaag gagccatacg
     5941 atcgttccgc cttcttcact tactgcttcg agtgtccatt ccggggaaac tcctttgagg
     6001 atgacagcct tgccgccaac tataaagctc ccgaaccaat gcattttcgc acctgtatga
     6061 tagaggggag gaataaggat gaaaacatct ttctttcttt gccagtggtg ggcattttct
     6121 gttatacagg cacacatcat atttttatgg gtaagaagaa tcggcttagg ttgtcctgtt
     6181 gtaccggacg tgaaatagag cccacacgtg tcatcaaagg tcaactctac ctccagtgac
     6241 ttggcagggg ctgtctccat aagttttttg aatgattcag cataggctgg tattttttca
     6301 ccgagacaga taaaggattt tactgtaggc agttgatctt tgatagcatc tattttcgga
     6361 gcaaactcct catcgtatac cactaccatg ggctctgcaa cctcggaaca gtatttaacg
     6421 tcctcagcag taaaccggaa gttgagtggt acgacccatg cgcctgttcg aacaacacca
     6481 aagtatgcaa caagccaatc gatagaattc atcatgaagt ggagcacttt gtcgcccttc
     6541 ttcacacctt ttgaggcaag aacgttggag aattggtttg cctggtcgtc aaactgcttc
     6601 cacgttattt cagttctctt tttttctgcc ggaacctttt caacaagagc aatatcatta
     6661 gggaatttac gtgcatttct tgccagcact tcaccgatat ggatatatat gtcagccatc
     6721 tatactacct cctcaattat ttgtgttttt tgctaatttt tgtattgaca aacaacactg
     6781 atcaatttat catgctaaaa aaattaacgc aatacaaaaa aacgcataaa aaaaatgtat
     6841 acaaaatgat tgtatacaca ataaggaggg ttttatgaat tttggtatgt tttccggtct
     6901 caatgcatca aagtttccga aacgcgagtt catcattgaa agctacgctg aaaaaggaat
     6961 caggagaagc ctgacatggg agcagtttga tgatcaggca aacaagtttg caaactacct
     7021 tatgaaggag tgtggggtaa agaagggcga cattgtcctt cacctgatga tgaactgtat
     7081 ggaatggtat gcaagctata tcgccgtgct gaaaaccggc gctacagtga cgcctctgaa
     7141 tttccgtttt gcaagcgcag atatcaaata tgctgcagat gttaccaagt gcaaagcctt
     7201 tctttttggt gatcagttca atgccagggt agaacccatt atgaaagata tggattattg
     7261 caaaaacttc atatgccttg gccagacacc tgcaggtgtg aaatcatatg aggaaattat
     7321 gcaaaagggt gatggcagca gtgtctgcgt tgacgttgat gacaacgaca tggcagaact
     7381 catgtttacc tctggaacaa ccggagcgcc gaagccggtc tctcatacac ataaaacgct
     7441 attcttcata ggtttgggca atgccctgac atacaacgaa ggttatagca gtatctatct
     7501 ttcaccccat cccttctacc acagcggcac tcttttcctg tccttcccat gctatatcgc
     7561 cgctggcaaa atactcatgc ccatggatct caagccagaa ttctatctca agtctatggc
     7621 tgacgaaaag tgtacaggag gatggaatac cgtccctaca tggtctgatg tgatcagcgc
     7681 aatcaaaaca gggcaggttg atatcaaaaa gtatgattta tcagcgttaa ggcatatcga
     7741 gataggcgca cagccagtgc cctacgtctt gcttgaagac tcgaaaaaga tattcccaaa
     7801 tctgcccatt gcaaacatct atggtattac agagggtggc ggtggcggaa cactaaactg
     7861 ctacgatgaa gatattatga gaaaaccagg atctattggc aaggcaacgg tcttcatgga
     7921 ggcaaaaatt gtagatagcg cgggcaacag gttgacaaac ggtgcagtgg gagaactcct
     7981 tctcaaaggg ccaagactca tgaaggagta tgcgttcaac cctgaaaaaa cagcagaaac
     8041 catcaaggat ggttggctct acaccggtga ccttgcctat gaagacgaag aagggtttat
     8101 cttctttgca gatcgtgcta aagaccttat tattagagga ggagaaaata tcttcccggc
     8161 agagattgag gacaccctca ggaaacaccc caagattcag gatgttgcag tcctcggata
     8221 ccctcatcca aggcttgttg aaattgttat ggcgatagtc cagccaaaag aaggcgagac
     8281 aatcacagac gaagaaatca taaactttgt gaaagataag ggccttgcca agtacaaatg
     8341 gcctgagaag atggtctata ccactatccc cagaaacccg gccggtaaga ttgaaaagcc
     8401 aaaactgaga gatatgtatg taaaacctgc caaagaggcc atggaaaagg aattcaagaa
     8461 acagtagtta aggcggattc acaaaaaaag cccgtcatga cgacgggctt ttttatatca
     8521 aaaccctgta atgatagttt caacggccca tgagggttat tcatcgacat tgctgatatg
     8581 ttccgcgatg acctggcagt gatcagatat acgttccagg tttacaagaa catccacaaa
     8641 aatagggccg gcctcagcaa ggcacacctt ctgataaaat ctttccaggt gtttctgtgt
     8701 tgccgcctta atcgccaaaa gaacttcaag gtggcgttcg aaaacatcct gtatcctttt
     8761 ggtatcccgt tgctcgatga gcatggcagc atcatcaaga ttcctcacaa caagtttgct
     8821 tatctcgtcg agttctttct tggctggctc tgaaaattga gcctttcttt tatgtcttga
     8881 ttctgccaac tcagctatgt tggttgaacg gtcaccaatc ctttcaatat cataaacaag
     8941 agaggagaat gcaaacaatc tctttgagag ggccggcgta aggtggttac atgagagatt
     9001 ccagagatat tgagttatct cagtctgaac attgttaaca acaagctcga tgtaggccaa
     9061 atctcgttgt ttcgctggtt taaacttatc gatgagtgaa atgctcgtaa taagcatttt
     9121 ttgagtcagg gcgatctccc tcccaagctc tttgtttaca catgcgagcg cttcttctgg
     9181 agacacaaga catttttcgt caaggaattc aggccataga ggtaaaactt gatcagttcc
     9241 aggaataatc gcttcaagaa gccgggagaa tggtttaagg ataaagatga agatcattgc
     9301 aatcgtgagg tttagaagga aatggctgta tgcaatctgt tgagcaacgc tgcctgacat
     9361 tttctttacc gacaaaacaa agaatggaaa gagagccagg cagaggacaa cgccaaagac
     9421 cttaaacata agatgggcaa tggcagtcct tttgccgttc acatttgtaa cagtggcctc
     9481 catgatggct gtgaccgttg taccgatatt tgcaccaagg acaataggaa gggcattctc
     9541 aatgcttatc agaccctgtt gcccgagaat gatgagggta ctgaccggga tggcagaaga
     9601 gtgaacaatt gcagtgaaaa taagcccgat agcgagacct ataaaggggt tttttgtatt
     9661 gcgaaaaaaa tgcaggaaag cctcgttctc cttgagagga gatgtggcat cactgatcag
     9721 gcttaatccg aaaaatataa tcccgaaata gaagactgct tcgccgattg ttttccactg
     9781 ctccttcccg gcaaagaaaa ggaagccgcc tgcgaagatt atgagtggtg aaatgcttgt
     9841 taccttccac acgacgagtt gagcagtaag tgttgtacca atatctgcac ccaatattat
     9901 gccgagggaa tggtagaagc tgacaaggcc tgcactgaca atgccaacgg taacaagtgt
     9961 tgtggctaaa ctgctttgaa aaaggatagt tgaaactatc ccgagaaaaa gaccgtaaat
    10021 tggcctcctg acagacatac gaatgtaacc cctgattctg ccactgaaga gcccccgcat
    10081 gagggtactc agcttgatca ttccaaaaag aaaaatcgtg agaccggtta tgaaaagaaa
    10141 aaggctatta atcatctttt ctatgctcgt tgttctgcat tctgccacac agatttcaaa
    10201 aggtaatgaa tcattcggga tggatcggca tagcctgacc tcccctgatc tctcacatct
    10261 ttctcgagat atctttgtct gaaagagata tcaacgatat tcaacgggaa gtatgcagag
    10321 gcccttctat attgatccac aaggctaaat ttttcctctg gcggaaactg tcctgtaatg
    10381 ttgattctat cattaaagtc cagcgccatt tgatacagag ttaatttctc aaaatcaaac
    10441 gaatcaatac acttaaattc aaacaaattt atgatatctt tgcccccggc gttatgtccc
    10501 ggtcaagagt cagcaaaacc actgaagacc tgtcttcgct ggatgctgcg agaagcatgc
    10561 catgggattc gatacccctc agcttgcgtg gttcaaggtt tgccactacg gctatttttt
    10621 tacctaaaag ctcttccttt gtgtaatgaa gcttgattcc agctacaatt gtccgttcct
    10681 gacccatgtc aatagtcagt ttgtacagct tgtctgcgcc tggcacatct tcacaagcct
    10741 taatttccgc aacccgcaaa tccaacttca tgaaatcatc aaaagttatg ttgtccatgg
    10801 attactcctt tcattaaaat ctgtttagcg cagagagcat agcaaaaact tccaagattc
    10861 tacatatcat atttttggtt ttgcatttgt cagtttaagc ttcttgacat caaatatact
    10921 acgccaaacg catcacagta tttttcagta tcccgatttc ctctatctcc acctctactg
    10981 tgtcccctgc ctgcaattct ccaacaccgg aaggtgtgcc tgtgataata atatcccccg
    11041 gcatcagggt catgatgccg gagatgaaag aaaccagttg gaaaacattg aaaatcatct
    11101 ggttcgtatt cgatttctgc tttgtgatgc cattgaccct tgtggcaatt gcaagatttg
    11161 tggggtcgat attttttgca atctttggac ccagtggaca aaatgtatcg aaggattttg
    11221 atcttgtcca ttgcccgtcc attcgctgaa ggtcccttgc agttatatcg ttcgcacatg
    11281 tatatccggc tatatagtgc tcagcatttt caactgccac attccgtgtc tttttgccta
    11341 ttactatggc aagctcgccc tcatagtgga gttctttggt ttggggaggg tagataatag
    11401 tttcggcatt gccaataaca gcggtttggg gtttcataaa tatgaggggg tatgccggca
    11461 gctccatatt gaactccctg gcatgatcaa ggtaattcag gcctatggca ataatctttg
    11521 tgggatgaaa cactgccgac ctcctttcgc gagcgcataa ttcaaaatgc cacgatcaca
    11581 tcatggtata attttataat cattttagaa tgttggaggg gaatttgcaa atgattaatt
    11641 cccggcatga ctgttaaagg gatggagcat gtacttgctc tttcgtctgg ccctaaaaga
    11701 agatattcca ggacaacgca ccttctgtct gataaaatga acgggttttt gttcgtgaaa
    11761 tctgaaaagc caatcctgtg ttcgagctta cagtgtaatt tgcctgcacc attgcctcaa
    11821 aataattgtg gtagtcccca ataccataat agatattccg ggcacaaaca tggaatttga
    11881 ataaatccgt cacctttgct atcaatccaa cagatgcccc tgcccctaaa gcatacattt
    11941 ctttcatggc gccgcttaca ttcagggcag tctctgccat ggcataccag agtcctgtgg
    12001 cgttgtgctt atacgcaaaa ccaccaccag gattgagctg gtaaaccata tgttcccgac
    12061 cgtcttccat gtacctgtgg gtcagaccgg tcttgatctt ccaggaaagg ggctgaaaaa
    12121 aaaggcctct cggcgtcagg gatgtgatgt caataatatc aagggattct aacctcagcc
    12181 tgttgaggga aggataatat cggagggtag tatcaccaaa tactatctgg gagccttccg
    12241 ggtacccctt gtcgttgtct aaaagactgt gataggcagg cctgtagcgt aactcataga
    12301 aaaaacggtc tttttttatt ccagtcccga ggctcaggcg attcgattcg tgcccctcgt
    12361 caggtcgtgg cggctggggt atggcatagc gatcctcagt tgattgtccc agagtacttc
    12421 tgatttttaa cagctccaga aagcggtcaa ggtactgatg ctttgaaatg cgtttttttg
    12481 tgtagaggta ttgaaggtat tcgatggcca gatcacacgt cgtggctttc tcatcctgag
    12541 agatcgcctg tacaagaatg tcctttggtt gtatctcgcc gtttgccata gcacgggctg
    12601 attcacggtc cttttgtgaa agaagagaag caacatactg aacctttgtt gtttttgagg
    12661 gtctgtaaat tgccttattg gtcaggccgt tcttctttgc cagcctgatt gtgtcgatag
    12721 ggattaccca taagtccatc tgatcggtga tattgaggga tggtcttcct gcatcgagga
    12781 gaaagagaag gtcataagag cagttctcat cgaagaaata gtagtccgaa taaattgagt
    12841 cgagctcgta aacgtgcata aggagtctcc gggtctcctc cttggtgaaa ttgaggggat
    12901 attcccagat gtccctatgg tctatatcgc tgtattcctg taacttgcca tagtaaggga
    12961 gaatggaaaa atagcctttg tagagtccaa acaagccttt aacagcaaag aggggtccaa
    13021 aggtgtctgt agtaatggca gagtagttga ttgcgtgaga gagaagcttg ctttgtgtcg
    13081 cagtttctat ggtgagaaga gtgtggccaa acatggaagc agggctgttt atgtgagatg
    13141 taggaaagat caatgagact gcttcgggtt tgatctctac cataagctgt tcgaagtggg
    13201 tgcactcagg tacagggagt tgagaaggat ctataccaag ttgctccttc aaccaggtgt
    13261 aacgtgcaac aaatctgcac accggatgtt ttgttccttc cccgggttcc tgaaaaaaag
    13321 ccttaatagt tgcctcaagt tctgcttttg gattgtgctt gccgccaggg gcaagaaaga
    13381 aattggggtc atctatgagg ctgcgcaaac caaaaactcc cttcttataa tggagaaggg
    13441 tgtgccagta cttatcatta tacaatgccc tgcggtctgc ctcctctatg agggcgagat
    13501 catatgtggg attgccagca ctgacctttg aaaaaatgat gaaaaaggat atgaggcaga
    13561 gaatgaaaag ggagatacgg aacatgtata ctctccgcat ctccctggca aggtttccta
    13621 tgggacaaat cacctaactt gacgctatta cagagtctat gtttctcaac acatcaaggt
    13681 agcttactga gtcggaggtg tagatttttg aaaaattggc ctgaagtgac gcatatatgg
    13741 ctgttttttg tgattccggc actgcgatga ggcctgcaaa cgtgtcaagg tattccccgt
    13801 gtccacgggc tatgtctttg gccaggctgt ccatgttctc tgccacaaat gtattgatct
    13861 tctcgtttga tgcaaaggtt ttgaactgct cgcagtttga tgttccggac gtaatgccga
    13921 atgtctggtt tccaaaggtt ccgtttgtgg tagctgcaga aacctgagaa atgagtccgt
    13981 cgtttccttc aaaagcaatc gaaccgagac cgcaaccgta attcttctga tctgcccaca
    14041 cagctgtgcc aagacagatt acagcaagtg caacgagtga aacagtgacg acttttttca
    14101 tcatgccctc cttattgggg tttcgtagat ttaaatatag tggaagtata tgtgtgggaa
    14161 aaagcagtgt caagcatttt tcttgcaaac atggctctat gtggacagtt ttttcccaac
    14221 ctttttttct acggatacaa ttttttgctt taatctgcct gcgctaccct ttcggatatc
    14281 aagcttgatt gttgagtaaa ctctattaca gtctttttcc aactgtctgt atgctttttc
    14341 gacaatcttc agggcatcat cgagcgactg aacctcaatg attgttccca tggctgtcaa
    14401 cgtgtaggga tgtccttctt catcgattac cttgaggatc cttcccacgt actcgctgac
    14461 actctcgccc ttatcagttg gaaacatggc aaattccagt agtacggaca tgcacacctc
    14521 cttattgttc atgtatggca attatatttc agaaggattt tattttcaac gcctactgag
    14581 accattgctt tatgctgtct gcacaataga tgtttttttg cctgttcgtc ctggacgact
    14641 ctgctatact tattaatagt gatggagcct gtactatgag agttttatct gattccgaac
    14701 agatatattt ggatcgcatt gatgctctgc aggagaaaat ccatgatctg gagaagcggc
    14761 tcgaaagatt tgaacgggtt gaagaagcgc tcagggagtc agaggaacgc tatcgaagcg
    14821 tcattgacaa tgttgaggta gggattgccc tcataagtcc tgctatggag ataatttccc
    14881 tcaacaatca gatgaggaaa tggtttcctc tcatccatgt ggacaccagg cccatatgct
    14941 tcacagcatt caacgaccct cctcgcgact ccgtctgtcc gtactgtccc acactgaaaa
    15001 ccttgcttga cggtaaggtg caccagtcag taacaagaac gccatcaggc ggtaagatca
    15061 ggcacttcaa gatcattgca tctcccttga gggataaaaa aggcgaagtg gtggcggcaa
    15121 tcgaaatggt cgatgacata acaaggagaa aacacgctga aaccctcctg agaaaggaaa
    15181 aaagagtctt tttctctgtt ttacagaaag ccccctacgg ggcagtcatt attgacaaaa
    15241 gaggaaaata tctctttatc aatgaagaat ttacgaaaat aacaggatac gatattgcgg
    15301 atgtccctga tggggtaagc tggtttctca aagcatatcc tgatccggaa agtcgtaaag
    15361 aagttctcaa aacatggaag gaagatatta cagagaaagg agtatcgaga gttttttcca
    15421 ttgaatgtag aaacggagaa aagaaggata ttgagtttag atcaaccaag ctaagtgatg
    15481 acaggtatct taccatgctc tctgatgtaa cagagcaaaa gagggctgag atggaattga
    15541 aacaggcccg ggatgagctt gaagcaaggg tggcgagtcg aactgcggaa ttattcaaac
    15601 tcaattgtga cttgcaggac aatattgctc tgctcaaatc aatcaatgca gaattgaaag
    15661 ccttctccta ttcaatatca catgatttga aaacaccaac catagctgtt gaaggttttt
    15721 cacagatact gcttgaacga tacgggcaca ggctcgacaa aaagggcaaa aattttgtgc
    15781 gaatgatcag ggaaagcgca ggtcaaatgc gggaactcat agacaacctt cttgcatact
    15841 ctgcccttgg gcgcaaaaaa ctcaagctct ccatgatcga tatggaaggg atggttatcg
    15901 aaatttttaa ccagttcaaa gctgcatatc ggggacaccc tatgagtatt tctattaaac
    15961 ctgcccctcc agcaaatgcc gataaaacca tgattcgcca ggtgatgctc aaccttcttg
    16021 gcaacgccat aaaatacagc agggtcaagg aacagtccga tgtcaaattt ggtggttggt
    16081 ctgaaccagg aagggttgtc tattacgttc aggataatgg ggtggggttt cctatggaac
    16141 agagtaaacg cctttttgaa gtgtttgaac ggctgcaccc gcccgaggaa tttgaagggg
    16201 ccggtattgg gctcgcaacg gtaaagcgta tagttcgaag acatggcggc gaagtgtggg
    16261 ctcaatcgac cattaatgaa ggttctacct tttatttttc catccctatc ttgtcgtgag
    16321 caacaaccag tgtggccatc cttgctgtgc catctatcat accccacctt acccttgtta
    16381 cttggcttgt gttcccaaaa gttaagtgcc ctttcttgaa ggtccataaa taatttaact
    16441 gttaaagcag tagatcccac gtgtaagtcc ctaggattgt accccgttgt ttattggaaa
    16501 gcatatatgc catctgaatc ttcagagtca tgtctaatgg ggtatccagt ttttcattcc
    16561 tgaacaggat caccatgaag actcaagata tctgtgtaga gagtgaggtg ctccagtttc
    16621 tcttcgggcg ttcatcagaa gagctgcctg tttgggaaca gaagtgaaca ctgcctttta
    16681 aaatttttta aaaaagggga ttgacagaat tttaaactta taatatcatc gaaattagat
    16741 acatcatctt aagcatttat caaaagatta ctagttattt tgtggcactg ttgttttgtg
    16801 aggggggatg tttcatgaat ggcacttgcg agattttttg gtcctaataa ccctataatc
    16861 ttagccaggg ggataagatg cgcaagtctg ctaagtgtac aattatcatt tctgcaatca
    16921 ttggtgtttt actgggattg taccttgttt ttggttacat ccggacaccg gttattcatg
    16981 ggaccgtcct tgatgcagaa accaagcagc ctgtggaaaa tgcctgggta acaggaactt
    17041 tatctctgaa ggtcgctacc atacaagggg atatccacgt ccatcccgct tttgcaccgg
    17101 cacatctgag aaccaataag gaaggaaaat tcattatacc ccgaaagagc tttagacaac
    17161 ccattcctcc tttaggattt ggtatgaacg tcgagggatg ccgagtaacc gttgagacca
    17221 taaatgataa gcaaggtgaa atcagtctta aaccatcttt ctggaagtgg tggactgaag
    17281 tgaccatata cgtgaagcca acattgatga cagaaggcga gtatgattca tacctacaat
    17341 cattgtttcg ttattgcaca acgggaaggt cgggagtaga ggtgcctgta gctaaagaag
    17401 ggtgtgatgc atgggaattg gattatgcca ttgcaaaaca tgaatatttt ttgaaaaact
    17461 taggtcaact ggacagttct gacaaacgga ttctatattc tggaacactg cataacctag
    17521 cctatctata caaagaaaaa ggtgattatc gtaaagcctt gcatgcattc acggttggaa
    17581 gagactttga catgaaaagg aacatcgacc tttggattag agagtacaat tcacaaatcg
    17641 aagaacttaa gaagattctt caaaaataat ttgtttatgg aaggattttg tatgcaaaag
    17701 aaattaccta caattatttt attgtttgtt gttgtaagct ggcttgttcc gttagtgcat
    17761 gggtttgaca acgcctccaa ggctccacaa caggtcaagg tagagtacac gagtcagatg
    17821 gctggaaact tccttggtgg ccacgataca atcaccgccg aggggctgct cttaaaaaaa
    17881 caggtgcatt atggtgatga taggtttaaa cagtgggcag accgcgatgc cgcatcaccc
    17941 ctccgtattg gtgctcacga cgaagatacc aaccagctgt taaattttat tctgcgagat
    18001 ccccccttag ggccaaacgg ctggggagat ttttatagtc acttttatga tagcagaatc
    18061 ggggatgatg ccgagaatgc aattggtcgt caggccacaa aagctgtcaa ggactatctt
    18121 gaaaaaataa aggaacttac caactgtacc tccaaaggct ttgagaccct ttcgccgggt
    18181 gataaaacaa gagtatacga ctatttggga agaatcactc acttatacca ggatatgttc
    18241 tgtccgtcgc atgtcaaggt agaggcacat ccttttcatc acccctttga acagtatatt
    18301 aacgataagt ggggcgagat tatccgatct tcctcattcc aagatcgagt aactaccagt
    18361 gcatacctca agggtaacta cgatattggg tcgagcataa atccaatcca agccatgaaa
    18421 gccacggcgg aaaaaagtag ggccttctgg ggtgaagagg cgctctgtga cctctgggat
    18481 gagcagaaat cgtcatgctt gcttattaat agtgaaaagc tacaacagaa tgtggatttt
    18541 ttagtcccag aggcggtgct tagcactgca gggtacattg attcaatcta tagagctgcc
    18601 aaagcgactc ccgattatag gaacatatgt ttaccaaaac cgcttcaagg gccggggggc
    18661 gatcatcccg atgacaggtt tgatgtgagc gatgcattct attggaaaga ccggtttaat
    18721 ttgtctgaga tgaagttaaa tgatcttttt atgagaactg gactaaaaaa agggaagaca
    18781 ggcgtgtggt atgggaaaag actcaaagag ttgtatggaa agggcagggg agcaccatca
    18841 gatatgccca tatcagaaaa gacaaaaatt tacaacgaat tcaagacagt gcatcaaaaa
    18901 ttgaatgaaa aatcatcaag taaagactgg cttacctctc ctgacatagc attatttgcg
    18961 tacggttttt ataatgaagc catctcgtta atgatcaaga ttaatgagcc tgcacgcttc
    19021 cttgatcttg attttgatcc tgccatcgtt caggatcatc cggtattgct tattcctacg
    19081 gcaggtcttg ctggttatga gcaatcagct accctgaagg caaaacttga agaatatgtg
    19141 cgtttagggg gcacgcttgt cgtttttgcc cagcaacttg gacatcattg gaatatcctt
    19201 ccaactccag tcgaccttgc aacgggcgaa gccaagtctg tttcaggcta tggttatcaa
    19261 caagatcaaa actgtcagca gggctcagta ttcatcgaaa ataatcatgc catgctttct
    19321 agttttgcat ctgctactat tgatgttggt gttgatggtt acttgtgttc ctaccctgac
    19381 aacagcacaa tcttattgcg acgatcagca aacggacaac cagccatgat cttataccct
    19441 tacggaaatg ggcatgttat tgttacagcg ctttacactg attttgcgtt ttctcgtggc
    19501 caggcaaata aatatgaaat cgatcttgtt gcgaatatta tttcatgggc gaaaaaacca
    19561 gcagaactcc ctcaagttaa acccggtgaa accatatcct taaatatccc ggttaccaat
    19621 aacatggata gtaatgcatc ttccgtcaaa ttaagaatat ataatcctac caggtcattg
    19681 cttcttagtg aacaaattat cgcaggaagt gttccagcat ctgaatcaac tatttttgtt
    19741 actcagttta caatacctgg aaatggagaa ccgggcatat accgggctga ttatgtacta
    19801 ctggataccc agggtgacat tatccaaccc caaacagaga atgactttag tcgctttgtt
    19861 gtaaacaagc cctcaacaac gggatctccg gataaaccaa tctggttttc tgttactaca
    19921 acatctcaaa atgttccctt tggtgcaacc tttgactata ccttccacgt ctataataat
    19981 tcagacgaaa taaggcacct caccataaaa agcatttttg gacatacaaa aagaccccat
    20041 gaatggcatg ttactgttcg gcccaaagcg gtgacgacta ttacagggtc agacattttt
    20101 gaagacaggg catatatgta tgatactatg cgcgctttct tatatgatga gttgaatcag
    20161 atgatagggc gatatgaact tagcttcaaa ggtttttatc cccatgctta tattaaattg
    20221 tcttcaaata aacaagtata ttcaaataat gaggatgttg cccttaaagt tgttaccacg
    20281 actggccaga ctggcgcata tactatcaaa acgaccatat taggccctga taatacactg
    20341 caatttgaag ataataggac gttaaatctc tcatcagggt caaacactat aattgatata
    20401 caccatcatt tagcagtaaa cagtaatcca gggtcgtata ttgcacgctc agaaatctgg
    20461 aaggaatcaa gatttttgag ctcagcaagt acctcatttg agttacctcg aagtcagatt
    20521 gtaatcacat ttaacgaacc gcaatctttc aaagttggcg caaacttgtt atctttcggt
    20581 ttaagaaata acggcaaaat tgatatacga tccggaacat ttactattac ccttactgat
    20641 ccgcacagta cggttatata tgagggatca cattcatttt ctctaacatc tggaaaatca
    20701 tcaaccctta ctattccaat ttctattcca aacctttatt tcggtgacta tattattacc
    20761 tatttacaat ctgatgaaac aaaaaatgga agcgttgtaa gtcttcacat tcccaattcc
    20821 gctacaattg gattactgct ggataaacca tcataccgta taagagaatc tgcaaatatt
    20881 tcagcaacaa tcataaatac tggtaaattt gatctcgaca acctccaagc aactttgtct
    20941 gtttattccc ttgcttttca aaatacaaaa atagcaaatg tcgcaaaagg ccagaacgtt
    21001 gccctaagtt tttcaatacc gattcctgag aatgcctggg ctggatcaca ttttgcagag
    21061 ttataccttg cagcaccatc aggcgatagc atgcagcaaa ctaccgttgt ttcaatccct
    21121 gattcatccc ttaaagtgca tttcaatggc ccaaacacct tgaacccggg agataatata
    21181 gccctttcta tcgaaaatat tggcggggta gatgctgaat acatagcgga gaaattatcc
    21241 ttgacagaca ataatggtac cgcaattcat aatggaacag caaatggaat agttcgtgtg
    21301 ggagaggtga agagctttta tgaattccag ataccgaatc aggttgttcg tggcgatgtt
    21361 atgttaaacg ttgcggttaa ggataataac acagggctaa taactaattt tttaaagtct
    21421 tttttattga gtggccttga ggcaagaatt gaaacaaaaa ctgataagaa cctgtatgca
    21481 attggcgaat cgaccatgaa taccacagat attataaatt taggttcaca tatcgaaaac
    21541 gcaaacctcg atcttttggt atacaaacta aaggcaggtg ttgaagggca gtttagccat
    21601 gttcttccaa aaggatggtt gccgcttatc caaccaacag atatagcttc agactctgaa
    21661 ggttacttat atgtagttga cgttgaagac agacgtctcg taaagatcca aaaaaacggg
    21721 agttttgtta aagagtttaa ctacatgaac agatgcctgg gtggaggaag ggaggtgtgc
    21781 cagccatata gcgttgctgc aagccctgac ggattcattt atgttgttga taagggtagt
    21841 tcatggtacc tcaatattca taattgtata cataaatttg accgtgaggg aaatttcctt
    21901 gattcctggc cttattgtaa ttctggtcca ggttttagct tggatcaacc ttcaggcatt
    21961 gctgttggtc gagatgggtc tgtttatgta acggatacat ataaccatcg cgttgttaag
    22021 tttagcagtg agggaacttt tatttcagct tggggcagta ggggaaagga acccggcaag
    22081 ttcgtatacc cagaaggtat tgccacagac agcagcggaa atgtttatgt cactgatact
    22141 gaaggttatg gctacaattc tttatgtagt catcgtgtcc agaaattcga tatggatgga
    22201 aacttcatta cccagtgggg aggatatggc acaaaagatg gattattcat gaatccccta
    22261 ggtattgctg tcgatgcgaa tggttatgtt tatgttagtg actcatctca gagcgtaaac
    22321 aagagctatc gaattcaaaa atttgacagc aacggtgtat ttgtcaaatc gtggggaggc
    22381 gaaggtagga ttgaaggcaa atttgtcaat ccaaagggat tagccgtaga tgaagatggt
    22441 tacgtttatg tagctgatag tggtaatggt agaatccaaa aattctctgc tgatggagat
    22501 tttatatcat tgtggggcgg gagagggttg acaaatggat tcctggccgg tcctgaaagc
    22561 ataggggttg atgctcaaga caacatatat gttgctgatc attctaatta tcgtattcag
    22621 aaatacgata aaaatggaaa ttacataaag cagtggggca gtatcggaac aggagatgga
    22681 cagttctttt gtggtcctaa gagcatcgcg gtaagcaagg aggggtttat ttatgtccca
    22741 acttgcgaca gaaaaatcca aaagtttaac agcgagggag attttcagac aagctggggg
    22801 gaaaaaggta cggatgatgg tcaattctca ggtttactga gaattgcggt aagcacgggc
    22861 ggttatgtgt atgtatcaga tgatgaagca aagcgcatcc aaaaatttga cagcaacgga
    22921 accttcattg caaagtgggg aaatcgtggg agtggccctg gtcaaattaa tgagataagg
    22981 ggtataagtg taagtcctga tggaatggta tatgttgctg atgcccatag aattcaaaag
    23041 tttggcgaag atggcatttt tcttctggag tggggagcag aaggaacgaa tgccggtcag
    23101 tttaaatcca tcgctagaat aactgtagac tccatgggat ttgtttatac cgtagaactc
    23161 aaccctattc gtattcaaaa attcgatggc gcgggcaatt ttataactca gtgggtagaa
    23221 ggggaaggag atatagtgga cgcgtttcgg tctgtttctg ggatcgcaat tgacagtgac
    23281 ggatctatcc tgatatctga ttcatataga caccggattt gcaggttaac ccctcttgga
    23341 actgatgtag tttttcaaaa aagcttctca ttatcgcaac ctggcaatac caatcaacaa
    23401 tacaacaaca ccattggagc gataacaaga tgcggtacgc attattgtga agccacgttg
    23461 aaaaacagtc tcgggcaaac tatctcaaag tctttttatc cgttttatgt tgcatcggga
    23521 gacgtggcaa tattgatgtc aatcgagaaa aaaaactata aaccaggtga gcagataaga
    23581 atatcaggcg aggttgtaaa catgtctgat acgcccctgt ccggttacaa cctcaacatc
    23641 acaaaactat catcccttgg agtcaacgaa ttgtttctga atgattttga tcttcctgca
    23701 cgaggtatat tcccgttctc tttcacattc agcgagttaa gagagggtaa gtatgtcctt
    23761 acggcaaccg tacaaaatgc aagtcttaaa gttgctgatg cttcagagca ttatgaggta
    23821 catataccta cgctttccac tgagattatt agtccagaca ttgcaagtga tcaacccttt
    23881 tcgttagatg tggtcatgcg gaacacagga gatgttgacg caaatgttaa cctatcttca
    23941 tccatcaatg aagaaacatt ttcgttaagc atggcgcctg gctccaccag ggttataacg
    24001 tattctcaac acataagtga agatacgctt tacagattta ctatctccgg tgatgttaat
    24061 gaaactttgg agaaaatggt ttatcagggg ttagctactt cagtctacgt atatgcagct
    24121 aatgtctatc cagaaggtaa ggtggcgatt cctgtttctg tagagaaccc cggtctgctt
    24181 gatgggaaga taagcctgga tatagatctt caaccgacag gctactcatt ccaaaaaaat
    24241 tactttgttc cgcgatggag ttctgcttct gacaacctta tttttgatct ggcagaagga
    24301 gaatacaccc tgaccctgac cagccaggca ccaccggcaa gtacaaccgc ttcattttct
    24361 gtgagaagag agaacaacgt gaccatggcg cctcttgtca tcggtacact gacaaatggg
    24421 tttgttgccg tgtccagtga atttgtaaac catggctatc gcgatgtcaa tggtacgatc
    24481 cagttgtctc tcctgaatgc tcagggtggt gttgaatggg agtcatcaaa cgaggtaaat
    24541 cttccggcaa gtctttcacc aacgcctgtt cctgcaaacc tatcttttcc ctcttcaata
    24601 atacaagtag ggaattatac agttaaggta gtccttctga atgaaactca acaggaactt
    24661 gcttctcaga cagcacctat tgttatctca ggtgccagcc tccaccttgt tcaggttccg
    24721 tcaatccaga cggtgccggc aggcgaggag gcgatgttca cctttgtggt ggcgaataca
    24781 ggaggaaagg aagctcaggc agaattgacg ctcagatccc atgatttcat aaacctcacg
    24841 caaagagtta cagtaagccc aggtgaacaa aagaatattc aatttgcttt cccgatccct
    24901 gctgaccttg aagaaaagga ctactacgcg gaatatgaac tcaaggacag ggacagtaca
    24961 tcagtaaagg ggcaggtaaa gtatcatgta actggcatca atatttctgt tgcagcttct
    25021 ctggacaagg aacattatcg ggagggtgaa actgcacacc tcgcattgat cgtaagcgag
    25081 caatcaggcg gagggacacg caatcttttt gccagggtca actacggtga ttttgagagc
    25141 aaacagaatt ttaccctaac aggaaaccag aacctctctt ttactgtccc tcttacggca
    25201 attacagggg agaaactgtt ttttggcatc tatgacgaag gaggaagatc gattcatctc
    25261 aattccctct acatttataa agctggtgat gccctgacta ttacaacgaa caaacaggtt
    25321 tacaacctgg gagaaactgt gaatatgaca atctcaggta ccggtatcgg taccatgact
    25381 atctctggtc cgggcggcta cagcgaaacc ttcctcttca gcggatctgt cgcgagaaac
    25441 tttgtgttgc ccacgtttgc tacggcgggg acgtattaca tagaagcaag tctggttacc
    25501 gcagaaggac agacggtaca cataaccagg ccaatcgaca tcgccggtat ttcagtgaaa
    25561 gtaaaggagg cgtcgcttga caaaggtaaa tacctgccag gcgaaccgct gcgcctttct
    25621 atgcgaatag aaagcaatag gaatgttgcg gctacattga aggtatggag tgtggatccg
    25681 gataagacct cgatttttct cggagagcat caactttcac taacacggga agaacaattg
    25741 ctcctcacaa ctgattatcc tacaaccatt actacaagcg ggatacataa aatcatatat
    25801 ggtatctatg cagataactt gcttctttcc tcaggaaacc tctcttttga tgcagggggt
    25861 ggggtaattc tcgggatgat gactgaccgt attgaatatc ctttgggttc tgaaacggtt
    25921 gccgtgaaat tcaacatcct tgggacaacc gatgcaacac ttactctcaa attaaatggg
    25981 attgccgtac atagtgaggc tatcaacctg aatggattca cctcagtcag ccttccactt
    26041 cctgaccccg gcccaggagc acacatggta gaaggtattc tctcaactga cgggctctca
    26101 agcagaaaag agacaagatt tctgtatgga acaaacttgc ccgaccttgc tgctgatgta
    26161 tggagttcag gcacatggat cggcaaagac gggacactca agctttttgc aacagtggga
    26221 aacagaggaa aaacaacaac atcgtcaaca tctatgaccc ttcacgatgg tggtaccatt
    26281 cttgccgctt tttctgtggg ggaacttgtt gcaggtgctt ctcaatcttt tgagtttctc
    26341 tggaatgtca tgggcaaagc aggggaacac acccttgttg cagctattga tcctaccaac
    26401 tcgataacag attttgccag ggataacaat caggtttcac gaaggattgt aatacccgac
    26461 attgcccttg tcacagagat agataaagac aattatcgga tgggtgagca ggtgacgatc
    26521 aactccacag gtatcaacct cactgcagga acgaactatc ctgatcttgt atgcacaacc
    26581 gttgtcaggg atctgtcagg taatgaagtg ttcaggcaga gcaagccctt tgggctatct
    26641 ccttcccaga taggggttgc cacatttaca tggagcacgt caagcgttac aactgaagga
    26701 aaatacacaa taaaacagga tattacttct ggtgcgactg ttcttgcaga gcggaccagg
    26761 acagttacaa tcgctgcccg caccggattt gacctaagag ttgatccagc gtcaatcaga
    26821 ttaaaacagg gagaaaccgg actttttaca gtctctgttg atgctccatc gggctggaac
    26881 aataatgtga gcttcgatgt tgaaggtgca cctgcaggcg caataataag ttttactccg
    26941 gatgcagtca ccggaccagg aacggcaacc ttatcaatcg tgacgagcgc ggcaacacca
    27001 cacggaacct attccattgt tgtgcgcggc cggaatggcg aagggtcagg agaggtcgtt
    27061 caggcaaccc ctgtgactat cgatatcagc ggcttcagtc ttgaggctgt gcccgaaagc
    27121 ctgtctatct ctcagcagga gactgcagaa ttcttggtca gtgctgtatc actgaataac
    27181 tatacgggaa atgtagttct atccgacgca accggtgaaa tagaaggaat gatcataaca
    27241 gtggatagcc ctacccttac agtgccggga ggacaggcca ttgtccgtgt tcagagttca
    27301 aaaagcctgc accccggagc atatacaatc aagattattg gaagtgatgg aataatcacc
    27361 cgggatataa agctctctgt acatgtgaca cagagtaccg ataccattcc tggatttgtg
    27421 ctcacgccag gccctggtcc taccaatcgg gcacttgtta cccttctgaa cagtaatttt
    27481 gaagagaaga tggaatttac ggccttcaaa accaggtttg gggcaaatgc agtgatggga
    27541 gatatcgatg gggatggagt ggacgagatt gttgttgcgc ctgggccaga tccgcttgca
    27601 gatggtggcg taagggtgtt cagacagaat ggcaccctgc ttttcgaaca acctgtcctg
    27661 gcgacaaaat ttggagccta tctggcagca ggagatattg atagtgacca gcgagaagag
    27721 atcgcagtgg gtgccggccc cggactcagc aatacagcac gggtcaaagt gctctcctat
    27781 aacggacaca agtttgagaa tacaggtatc gattttattg cattcccgaa aacctataag
    27841 ctcggggtaa aggtagctct tggcgatgtg gacggtgatg gaattttaga gatcattgca
    27901 ggggccggcc caagcccgtt gagccctgca cgggtcaagg tgtttaaagt agacacatcc
    27961 ggcggtgtgg gaaactggag cgtatcttcc acactctccg attttattgt cgaatttggc
    28021 gattggtatc cctatatctt tggtgtgaat gttgccgcag gtgatgttga tggtgacgga
    28081 atggcagaga taattgctgg tgcaggtcca aatcctcttc agaaagctgt tgtggcagtg
    28141 tacaggggtg acgggacttt taccggtacg cgctttgacg cttatccatc aaatgagtac
    28201 cgttttggtg taaacgtggc agcccgtgat ttggatggcg atggtctggc cgagattatc
    28261 acaggccccg gtccttcacc attcagtgat tcctggattc ggattttcag aggtgatgga
    28321 acccttctgt cagatggttt tctcgcttac ccggaatcta tgaaattcgg ctccaaagta
    28381 tctattggaa ctgtaggaga gtaaaaagaa ttctcagtcg cgtaggaaac cgattcgaaa
    28441 tcttgctatg agctttcagc tcacagccat gaactctctc aaattccttg acttctccgt
    28501 ctatttcctt taatgtattg gtgcggggtg gagcagtacg gtagctcgtc gggctcataa
    28561 cccgaaggtc tctggttcaa atccagaccc cgcaaccaat gatatcaatg gtcttgggga
    28621 tccctcagga ccttttttat tgccaaccgt agccaaagcg tacctactct taatccagga
    28681 aagcctgtac catatcaatt gaattgatat ggtatcaaaa aatagctttt agcctctcaa
    28741 tgtgaaaatc tcttacttca attctgtgaa ctgtaccaat ggtgcgaaga acattttcaa
    28801 cttggtaatg taaaacgatc aagttgtatg ccacctcgca gacccgtaaa ggttctccca
    28861 cagtgtcaga agatttttct tcacaaacag ccttatcctc caaagcattc atcaaaatat
    28921 agcgtgcaaa gtcactagcg ggcattccgg ccactgcatg aacaacgttt cttcgcattc
    28981 ccggattgcc atcgctgtat agctctgcca gatacccctg agattcatca ggaaacatct
    29041 gcagaatttt ttttgcgaca tggaattttt ttgcttcttc tatgtctctt gtttttttgc
    29101 ctgttttaag atggctgata gcgaatcgga ccgcttcatt tttcctgttc ttaaaagcac
    29161 ggtcgatccc tcttttgaga aactcatcat cataaaaaaa atcttctttt ttcaggtttt
    29221 gaaacacatt ctcagccgaa agattagcga gttgctttat ttttgcatct tttacaggat
    29281 cgaccatggc ttctctcgca tcaaggcgct cgaaaaaggt gcacaggatt aatccacaca
    29341 aaagaaccag gtaaattttt cttgtttcca tttttccctc caaccctatc cctcttttac
    29401 tgctgagatg gtaatttgtt aaaatcatag taatcgtaaa cagcttcgta tccttgtatg
    29461 atggtccata tttcacggtt gctcttgcct tcaagatatg cctttatgta tgcatcaaag
    29521 cccaggtgtg atgagtcatt cacggtgaag aaagcgatac cccgattcaa agtatccagg
    29581 aagtagttcc cggaattaca actgtcgtaa aaaagtcgcg agtaccgcat gttttctttt
    29641 tgcacactca aatctttgcg ggatacaacc gttttgtatt cgaaatgcgg tgacgggtat
    29701 accatattat caatttgttt gttgagaaca ccagctacaa ctgatgtaat tctcactcca
    29761 tctgcaataa ctcgccctga actcgcctga tcagacaaaa ccaccgagta attgccaggc
    29821 tcaaagtaga actggccaaa ttcgttccat tggccaccat tttttgtttg gtcaacaata
    29881 aaaatgttgc tgccggtagt atgatttaca gtatatcgcg cgccttttgt gtgatgagaa
    29941 gctgcaggcc accagccaaa tatcttgtag tatcctggtt cttggatggt gaaaagccat
    30001 tccaccctgt tttgacccgt tcctgcggaa gagtagaaat aattctccct gtagaaacca
    30061 gtaacttctt tggaccacgt ccattcgcca atcacattca gggaaggagc cgttggcgtc
    30121 ttaactatga agtatcgcaa ttcatcaggg tttgtgggat ctggagaacg gccgtcagga
    30181 gaataccatg caggtatatt ggaatctgga aaacgttcaa tagccccgtt acgggtggtc
    30241 tcaaccttat aaaggttcgg atctcctgga atcctgtatg tgatatagta attatagggt
    30301 ggatctatgc ttggatcact aaaatcatat ggcataatac cgcttgttcc gtcttgaaaa
    30361 tctgtccacc agtcggggaa ggagtggttt gttctaatag ctctgacagc tggtgctacc
    30421 catggagagc tgatcgtcaa tatttttggg tcatcaatcc agtaaatatc ttcaataact
    30481 tcgtttctta tttcgtcagc tgtcccaaaa acagggccaa gaccataatt tgaatgccct
    30541 aaaaataata tgtgagcttc ttcctgctgc aacgcaagtt tcattgaatc aatatcttcg
    30601 acgaaatgaa taagaaattc tttttttata gtcttatttg tatagcgcca tacatctcca
    30661 tctttttgtg cgcccatggt acggacgaga tattcaagac ctgctttctt gtcggcaagc
    30721 cacggcagac gatatccaag gcatatataa atatgttctg ttgttcctga agaagtagca
    30781 tttatagtca aattcctagt cgccggagct gaccaggcat tattgttatc cctgacccgc
    30841 agtgatatgg tgtgggtccc gggtaagagg gaggaggtgg agaaggtact ggcagtggag
    30901 aggaggccat tgatgcttga tgtccactca taggcagtaa tggttccatc aggatctgtc
    30961 ccgctgccgg taaaggtaac agtctgtcca gcagtggcag ggttggggga gatagaggta
    31021 atggtggcag tggggggagt gttggaggaa gagcctccta tatacgtaac cctcagctcc
    31081 gcattgttgg tttcattatc ctcggagaag tcagcatggc gtatggttgt gcccgattct
    31141 atggcatcgg atgccttgag tcctgctgac caggtaccaa ggccacccat ccggcaccag
    31201 gcaatatctg caactgcagc aggccctaag tttatccagg gagtccagga tcctgaaaac
    31261 ggttcaggga gggatgcata gggggtattg ttgatcttgc tgtagacctg agcaccggta
    31321 tctgcctgag ggttgtcctc accgtgggag gtgccataac gggtgaggaa gagtgttcca
    31381 ggggatcctg ccgtgctctc agtatagaac cgtacctcaa cagaggtgat cgtccaggaa
    31441 ggatcaatac cggaaatatt ccagcggttg actgccctca aggcaccgga gttacccctt
    31501 cctacccagt cggcgccatc gtggtagtat ttggtgccgc tactgtttac atctgtgctg
    31561 taggttgatg tgagggtaag ctgatattcg ctgccccctg atgatactat gaggctgtct
    31621 gtcgccggag ctgaccaggc attattgtta tccctgaccc gcagtgatat ggtgt
//
LOCUS       DAOOMC010000017        31201 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdus sp. isolate AMR_MDS_2685
            NODE_1235_length_31201_cov_6.133597, whole genome shotgun sequence.
ACCESSION   DAOOMC010000017 DAOOMC010000000
VERSION     DAOOMC010000017.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864760
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdus sp. (wastewater metagenome)
  ORGANISM  Syntrophorhabdus sp.
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae; Syntrophorhabdus.
REFERENCE   1  (bases 1 to 31201)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 31201)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,729
            Genes (coding)                    :: 2,708
            CDSs (with protein)               :: 2,708
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 7 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31201
                     /organism="Syntrophorhabdus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1235_length_31201_cov_6.133597"
                     /isolate="AMR_MDS_2685"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2699756"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            304..723
                     /locus_tag="PK125_02915"
     CDS             304..723
                     /locus_tag="PK125_02915"
                     /inference="COORDINATES: protein motif:HMM:NF019575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HPB37091.1"
                     /translation="METKSDILSGRYGHQKGFTLVELIIIIVILGILAAVAIPKYADM
                     KAEASNSTARAILGGLRDAATIVYANRIVNASTEPIDMTAVWSQAQVSGYDTDPIISS
                     DTLTLQISGQQYAYRLVKAGGLATGQPNVIIQNQPSW"
     gene            815..1216
                     /locus_tag="PK125_02920"
     CDS             815..1216
                     /locus_tag="PK125_02920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003111216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HPB37092.1"
                     /translation="MRTVSSAKGFTLIELIIIIVILGILAAVAIPKYVDMRQQAADSS
                     ARGILGGLRGAVTVIYADRAIKSQTTGIDMTAVLSQVQISGYDSSVPGANTLLINISG
                     NQYTYYLNNTDNIPTTPPSVTIQTGTGTPNW"
     gene            1347..3701
                     /locus_tag="PK125_02925"
     CDS             1347..3701
                     /locus_tag="PK125_02925"
                     /inference="COORDINATES: protein motif:HMM:NF021126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HPB37093.1"
                     /translation="MKNQHLDITLKNLITVSGLFFSISLLYFFPMLSSKNIFVERDLA
                     AFFIPPRYLWVSLVKSFQIPLWNPFNYAGIPLLATLQPGIFYPPHLLFFILPFNIAWN
                     WIIISHFFLTAVTTYSLLRYLRASHEGAILGAISVMLSGYLISVHNLLTHLLAVAWFP
                     LILVFFMRYFEEGKKKFLVFTSILLAIQFFAGAPEISFITVMGLSVIAIFINKFMDIV
                     PHFVSSSATRLKALGLVFSLFFLLSSVQLLPFLELKAHSVRATGLSYVEAVTWSFAWK
                     DFIQFFIPDAFGYFSSIKKYWSNQSWLLTLYWGITPFILSFFYFISTDKKRVVLASLM
                     FISLVLALGGNTPAYKLLYYIPPFSGIRYPVKFIFLFIFLVALTAGLGFDYLKKGVPR
                     GDARVRRTILAFFYSGFIFAILWGFMSIFGDAVTRFFESAGFIPDKYNEAKINIHNIK
                     RFLLFSFLFCVLLVFNLRVRFKKTVLFLVLLIAALDMYFGNAGYYTAGNWKFYIEPKG
                     WGTYTFFTKLSDNKNTDRYFVTFKTLNDFNYFPYDRTILAPPYASLFGLYTFGGAEVL
                     RIGHYENFANLLKDSPTIDSGKRFLDVSGIRHVITTQELTDNDFGLLQSIDMGSRTAH
                     LYQYLRYPGRFMLFGRMHAAKDNKEAIEKLLDQSIDLKKELVIQSTNGFILNESDVKG
                     SIRLLSYEANRVSLACKTNSDAFLYVSDVYYPGWKAYIDEKEVSIYRANLAFRAVYVP
                     KGSHTVLFVYRPLSFYVGFCLTLFGLVASCFLLIKNQGQKINYV"
     gene            3742..4302
                     /locus_tag="PK125_02930"
     CDS             3742..4302
                     /locus_tag="PK125_02930"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HPB37094.1"
                     /translation="MAKKRAQLADSLIPRPFRSGRILDIGCGMNPAFLQTTAFSEKYG
                     IDKAGSLSCHTKFDEQLITFIHHDIESGDPFPFYDEFCDVVTMLAVIEHISPNNLSKV
                     IAELYRVLKRGGLLIITTPSHWTGFILNGMSLLRLISRQEVKDHKELYASDDIMKALQ
                     LGGFAPGNMQQGCFEFYMNTWIMAIK"
     gene            4389..4646
                     /locus_tag="PK125_02935"
     CDS             4389..4646
                     /locus_tag="PK125_02935"
                     /inference="COORDINATES: protein motif:HMM:NF015126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-Tu/IF-2/RF-3 family GTPase"
                     /protein_id="HPB37095.1"
                     /translation="MEEVEIGKVLKYFSKIGVAAIRITDGELKTGDTIKIKGHVTDFE
                     QVVESLQIEHESVEKVEAGKDVGIKVGGVVREHDTVYVVKR"
     gene            5005..5589
                     /locus_tag="PK125_02940"
     CDS             5005..5589
                     /locus_tag="PK125_02940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB37096.1"
                     /translation="MEEQELTNRFCSLFEETLPILTNIYKGFAGQKINLLKESRSKFR
                     EVWKNRLPAVEKLIGDTEKNEVEKKFVALLPHLQRVALAIDNLVDKMEIKVETHILFS
                     QKALDEIKQLMVAVGTEFTDVKDYCMTKNPVLKEQIRTDMEKIRKMIYEFEMVHQNRL
                     ITGVCMPKASYLYIDMTDSLKRMAKELGDFAEKA"
     gene            5598..6962
                     /locus_tag="PK125_02945"
     CDS             5598..6962
                     /locus_tag="PK125_02945"
                     /inference="COORDINATES: protein motif:HMM:NF025112.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HPB37097.1"
                     /translation="MSSDNLTKAIHNQHVLNKISNCSFADKAFLVGGAVRELLLNKTP
                     ADYDLVLTRYGDLRILENVFGVSAFLLGKKPIQTYRLVQENISIDITFHAGAIEEDLD
                     RRDFTMNAIAYDFKERCIVDPLKGIQDIEARIIRYPHRKNLEDDPLRMLKAVRHFTML
                     DGFKMDENLFNAICILKHLIHKTAPERIKYEMDQIILSENAFLGLKMLQETGLLFELF
                     PELYALKKLDEEKQFILETFGHAIEGFKYLTKYGRAYELDERDFRNTGYALLLHDIGK
                     AHTFSHDELKNAVHFFYHEKVSCNLANHIMEKFRFSSIDIKAIIKLIENHMRIFLISN
                     SDSTEKALRRLVYKMGDLTPALIVLTLCDMYGSSAGTENDSTFMVQKKCAHVLRAYNE
                     WKKKPLPRLINGNELKAIGFEEGPIIGQILNEIREKQINEEITLKEEAISYANDRLKK
                     QQMH"
     gene            complement(6959..7342)
                     /locus_tag="PK125_02950"
     CDS             complement(6959..7342)
                     /locus_tag="PK125_02950"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPB37098.1"
                     /translation="MPKIYVLRDPLTPEEHITLGLSYERNGEYDAALNEYKTALKKLP
                     IASLYIGNICFQHNDYAEAEKAYKVAVSKTDDPRAHNNLAWLNYISGRNLVEAEMHAR
                     RAVELSPDNQDFMDTLIKIVEKRKD"
     gene            complement(7381..7923)
                     /locus_tag="PK125_02955"
     CDS             complement(7381..7923)
                     /locus_tag="PK125_02955"
                     /inference="COORDINATES: protein motif:HMM:NF015377.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HPB37099.1"
                     /translation="MKNCLFLCLLVVLLCLTGCATTGRITADARIIEGVPYFEQEDFQ
                     CGPAALVTVINYWHRKSRCNERLSVDAVAKEIFSNRAKGVLNLDLELYARKLGFTSLQ
                     FSGTIEKIKEYIDENSPVIILVDNGRAFYQQNHFMVAKGYTQDGIIFNSGREENHLIL
                     KESLITIWKKTDFWALAIKP"
     gene            complement(7984..8373)
                     /locus_tag="PK125_02960"
     CDS             complement(7984..8373)
                     /locus_tag="PK125_02960"
                     /inference="COORDINATES: protein motif:HMM:NF033919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PA2779 family protein"
                     /protein_id="HPB37100.1"
                     /translation="MARKCVVWCLISAMFILGIAPRLEAAFVPSQAINYMYSDRGSDL
                     KAIQSALESRLVQQRLADLGYSSDEIKDKLALLSDQQLHSIAQKLDSLRVGQDSGLGI
                     VIAVLVIIILIIILINLTTGHKVIVTK"
     gene            8586..9473
                     /locus_tag="PK125_02965"
     CDS             8586..9473
                     /locus_tag="PK125_02965"
                     /inference="COORDINATES: protein motif:HMM:NF015948.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HPB37101.1"
                     /translation="MNRRQFLKALSTSFGIIGMSGGLKHLFSDEPSVLSVAEGSDYAA
                     ITRSAIAALGGMQKFVKQGSVVVVKPNLGWDRKPEYAANTHPLVVKTITEECLKAGAI
                     KVKVFDNPCNDPRRCYENSGVADALKGIKNVQLKHMESERYKNVKLNGVFLKAWELYD
                     EALTADVFINVPVAKHHGLTRVSIGLKNIMGIMGGNRGFIHRSIEDALTDVNTVIKSH
                     LTIVDATRILLNHGPQGGDLKDVKALHKVVACRDIVAADAYATTFFGLNPQNISTTVT
                     AYKRGLGEMNLSRIKVIKA"
     gene            9474..10988
                     /locus_tag="PK125_02970"
     CDS             9474..10988
                     /locus_tag="PK125_02970"
                     /inference="COORDINATES: protein motif:HMM:NF024211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HPB37102.1"
                     /translation="MKITWSRISQVLFLATFFVLFVLTEYRGKDIISAAINSFFRADP
                     LVAVSYLLSQKTFTVVLLPGILMVLFTVILGRFFCGWLCPLGTILDLVTRYIPKKKHI
                     GFLKSRFKYYLLFCLLFAAFFNVNLIGILDPIAIVVRALTFFFYPLFGYAVRSTWAGL
                     YRLIGEDRDHVSFIFAFFRDHILPFRETFYPLAFLSLILFVVILVLEHYEKRNWCKNI
                     CPLGTLLGLLGRFSPFKRLLSRLCVDCKRCKDICPTGFDEEILQKEDCILCMECKLKC
                     ASKRVRFVLFRRSKERLGSTFVMERRVLIGGLLSGFFISKAFLFQSPGRHERLLRPPG
                     VNNEGDFLKKCVRCGECMKVCLRSALYPDLSKGGIYGLFMPVLIPRLGYCEYNCNLCG
                     QVCPTGAIPNLSLEQKKKSIIGLAAIDKNHCLPYAKKMNCLVCEEHCPIPGKAIHFET
                     VTDVDYAGKKVILKRPYIVDELCNGCGICENRCPIEGKSAIEVFSARKISTSKK"
     gene            11081..11365
                     /locus_tag="PK125_02975"
     CDS             11081..11365
                     /locus_tag="PK125_02975"
                     /inference="COORDINATES: protein motif:HMM:NF038144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PxxKW family cysteine-rich protein"
                     /protein_id="HPB37103.1"
                     /translation="MVCETLKPGVECLFMKKTGCSYNGGKCYTIVENCEGCSKVVEFE
                     TGKYCSSFPYPAQKWQKGSCSMSSHTKKSSQAAGQKINPLKASKRSAGKR"
     gene            complement(11572..11651)
                     /locus_tag="PK125_02980"
     tRNA            complement(11572..11651)
                     /locus_tag="PK125_02980"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11615..11617),aa:Leu,seq:tag)
     gene            complement(11791..12729)
                     /locus_tag="PK125_02985"
     CDS             complement(11791..12729)
                     /locus_tag="PK125_02985"
                     /inference="COORDINATES: protein motif:HMM:NF024816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPB37104.1"
                     /translation="MYRSVKKDRWYWIYKDDEPVLTPMNAPVITSHQLLADRLVSNLE
                     QYGETPSNPESIAAFHYTLIDFFLNTPRDENEYLIAKGLDEENDWTFRCPAAGQDCMM
                     RWWAVFGQPMSAEKKEEKRKWLSSLTLMQLCAVNVISRYTGSVNIACRLATSVDRSTL
                     RQYAGDIVAFYPYLRAKTLYQAFENFLFYFTLEGEVQPEDTTQFEKVPITRPSLEDHL
                     IVVADLYRTYGPDSPQYALGLNKLAGLYLAQCHYGKAERTYERALAIMERTLGPNHPD
                     IAPILGDMAVLYRKTGRDSDAKRIDKLAMAIRAPKR"
     gene            complement(13224..14063)
                     /locus_tag="PK125_02990"
     CDS             complement(13224..14063)
                     /locus_tag="PK125_02990"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HPB37105.1"
                     /translation="MNKHESKRGGIGLKRLVVSVCRIMVFVYLGTVLYSCLVSETMIF
                     QPQPSSYQDSDQIIKLDSGNGEAVSAIYLRNPNAFYTILYSHGNAEDIGHLLPMLEEI
                     KGMGFSLFAYDYRGYGTSLGTPSEKKVYEDEETAYDYLVNNLGIPACRIIALGRSLGG
                     AVAIDLAHKRQLGGLIIESSFVTAYRVLTRIPLLPFDKFKNISKMKKVRCPVLVIHGK
                     QDEVVPFWHGERLFEEANEPRLSFWVEGAGHNNLFEAAGSDYRKVLRDFVTIVEKGSK
                     NMP"
     gene            complement(14285..14413)
                     /locus_tag="PK125_02995"
     CDS             complement(14285..14413)
                     /locus_tag="PK125_02995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB37106.1"
                     /translation="MVAEKWPVLAKKYAEGWEWDAVFLDMPPSNTKTMETGSGRNS"
     gene            complement(15090..15311)
                     /locus_tag="PK125_03000"
     CDS             complement(15090..15311)
                     /locus_tag="PK125_03000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB37107.1"
                     /translation="MILSHIDILDKRNMQHRVKATIVANHPLSRYGQPVILLENGRAL
                     DKSSWFSHRYRVLKASKKEISALLSMGLV"
     gene            15643..15846
                     /locus_tag="PK125_03005"
     CDS             15643..15846
                     /locus_tag="PK125_03005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB37108.1"
                     /translation="MESMELMLVEQPKREVLEKDAVSVFPFFFPGMGNRLMGLRNNGK
                     KAYIVVDLSGRVFFYDREVSSSN"
     gene            16306..16509
                     /locus_tag="PK125_03010"
     CDS             16306..16509
                     /locus_tag="PK125_03010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002856364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate tautomerase family protein"
                     /protein_id="HPB37109.1"
                     /translation="MPYVNIKVTKEGVTAEQKARLIAGVTDLLRTVLGKNPATTFVII
                     DEVDTDNWGIAGEQVTLRRSRGM"
     gene            complement(16969..17154)
                     /locus_tag="PK125_03015"
     CDS             complement(16969..17154)
                     /locus_tag="PK125_03015"
                     /inference="COORDINATES: protein motif:HMM:NF018595.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HPB37110.1"
                     /translation="MKQVFIEDSCIGCGTCVEICPEVFELNGEVAVVKKGANLSLDKK
                     IIEAAEACPVEAIVYEK"
     gene            complement(17164..17508)
                     /locus_tag="PK125_03020"
     CDS             complement(17164..17508)
                     /locus_tag="PK125_03020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HPB37111.1"
                     /translation="MITIKRIYDPPSKDDGYRVLVDRLWPRGMSKEKAHVDLWLRDIA
                     PSNELRKWFNHEPQKWTGFKERYFAEIALQKELLEPLKKKMKETHITLLYGAKEPHIN
                     NAVVLKEYLEKM"
     gene            complement(17574..17711)
                     /locus_tag="PK125_03025"
     CDS             complement(17574..17711)
                     /locus_tag="PK125_03025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB37112.1"
                     /translation="MRTDVTVLCGSCKSEVEEITYIASGFTDDSTLTCGEDDTRNPKK
                     G"
     gene            complement(17805..17930)
                     /locus_tag="PK125_03030"
     CDS             complement(17805..17930)
                     /locus_tag="PK125_03030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB37113.1"
                     /translation="MAETRAVIKGINCQHSVVAAKKGLEGVLGILERDVPIRDFS"
     gene            complement(18010..18441)
                     /locus_tag="PK125_03035"
     CDS             complement(18010..18441)
                     /locus_tag="PK125_03035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB37114.1"
                     /translation="MKKLMVFSFVVVFGLTLTTAVFAAWNRGPWVNPGEQTDVNAFRN
                     FQKETLPLRDEMAVKRLEIRNEFNKETPDQAKIANLQKEMIDLRTKISDVAKKNGLSN
                     WGYGRGYYRGGWGPGYGRGHHMGWGNGYGPGYGRGYCPMWQ"
     gene            complement(19339..20682)
                     /locus_tag="PK125_03040"
     CDS             complement(19339..20682)
                     /locus_tag="PK125_03040"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HPB37115.1"
                     /translation="MVSRALLINPSIYDFAAYSFWSSPLGLLYIGGILRANDMEVNLI
                     DCMQIVERKRKVDGRAPFVKEKVESPPALKHIRKRFKRYGISRDALIRKLGESKEPDI
                     ILITSIMTYWYLGVKEALEAARHVFPSSKIIIGGIYPSLCSAHADRTLREADLIIKNS
                     EIEKFYGFVENAFDKKLTYKPSLYDLDHIPYPCYDLYKTIPFVPLLTSFGCMYRCAYC
                     ATPYMHPGIVRRSPGSVIEEVRHWHELGVSRYVLYDDNFLYKNEVYAKPLLKKICSLP
                     FQIDIYNPNAMNGALIDDELAHLLRGAGFREIRIGLESADPVVQKSTGSKISLKHFEK
                     SVTALIDGGFKNNEIVAYILAGLPNQRWEEVKQSIDYVLGIGIQPYIAEYTPIPHTRL
                     FDEYGQCARFPIWEDPIYQNNALFPFAWEGFSEKDLEYLKLYARDKRFPEHTNKE"
     gene            complement(20702..21382)
                     /locus_tag="PK125_03045"
     CDS             complement(20702..21382)
                     /locus_tag="PK125_03045"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HPB37116.1"
                     /translation="MIPIRDNIATRTYPIITVSIIFVNILIFLWMRFTLTGTEGQIVY
                     KYYGLVPREFMTSASSRLDLVPYNVMTIFTSMFLHGGFFHLGGNMLYLWIFGNNIEDA
                     TGHGRFILFYLLAGIAAAFAQLFYDPTSNIPMIGASGAVSGVLGAYLVLYPYARIKTL
                     LFIIIFIKIVELPAIVLLTIWFFMQVLYSGMEGVAWYAHIGGFVFGLIMIKLFSRKPL
                     FRGSKKRG"
     gene            21444..22445
                     /gene="galT"
                     /locus_tag="PK125_03050"
     CDS             21444..22445
                     /gene="galT"
                     /locus_tag="PK125_03050"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008108 - UDP-glucose:hexose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HPB37117.1"
                     /translation="MPELRKDPIIDRWVIISTERGKRPVFFIEEPPPPKAAMCPLCPG
                     NEHMTPAEVYAVRPGNSSPNSPDWLLRVIPNKFPALRIEGSLNKEGVGLYDRMNGIGA
                     HEVIIDTPVHGETLSNMSVRSIQNIFGAFKERVLDLAKDKRFRFTIVFKNHGTIAGAS
                     LDHSHSQLISLPVVPKRISEEISGSMNYYRQKDRCIFCDIIAQEQEDNVRVIYENKRF
                     ISLSPYASRFPFESWILPKNHESHFVFPSDDDGYRDAADALSMVLKKQDKVLNSPPYN
                     FMIHTAPLGSGDMPYYHWHIEIIPRLTKMAGFEWGTGFYINPTPPEEATTYLKEADI"
     gene            22454..23410
                     /locus_tag="PK125_03055"
     CDS             22454..23410
                     /locus_tag="PK125_03055"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR02095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch synthase"
                     /protein_id="HPB37118.1"
                     /translation="MKILIASPELYPFVKTGGLADVTGTLPKTLKKLGAEVRVILPKH
                     KGVEELNFPLHYKNFKIACQISQAYVDGEIVESEYDGVAAYLVEKDEYFYRDYLYSTP
                     DGDYLDNAERFIFFSKSILEAIKITGFVPDVLHCNDWETALVPFFLKTLYKDDPHLKD
                     IATLLTLHNLGYQGLFWHHDMHLLNTGWEYFTPDYIEFFGKINFLKAGIVFADILNTV
                     SKQYSEEIQTPEFGHGLDGILRTRKADLYGIINGIDYDEWNPENDRYLPANYSADDMS
                     NKSHCKKALQKTFGLPVNQNIPLLATISRLADQKGFDLIHYP"
     gene            23416..23916
                     /locus_tag="PK125_03060"
     CDS             23416..23916
                     /locus_tag="PK125_03060"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HPB37119.1"
                     /translation="MLTLNGGLQYIVLGTGDRKYHDIFTDVAKEFPKSFAIKIAYDNA
                     LAHLIEGGADMFLMPSRYEPCGLNQLYSLRYGTVPIVRGVGGLEDTITDFEVNPELGT
                     GFKFYDYSRTSLLNTIEKALKVYRRKKDWATLMKQCMAADFSWERSAKEYLNLYRKAI
                     EKHASD"
     gene            23903..25639
                     /locus_tag="PK125_03065"
     CDS             23903..25639
                     /locus_tag="PK125_03065"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPB37120.1"
                     /translation="MHPIDILGKIIAISHSNLEIASRINAILNIITQDMPFDEAIVYT
                     LDTDKKLTCRYKNDKSVLFPLLNEYRSHIGEGIVGSVAQKRIPQGFTFKDVPPRLGCL
                     FYPDLDGYIQKYRAFHFIPISDDSYLYGVLFICASSMERLNKPHNAFLSMVAREIGGI
                     LRSNQLLISSKKRISELATLSELGRVLASSSIPQDILNSITFIIAKALNAAFVTVRLS
                     HSFLKIDSQRFTYGEIGSESKEIIKKLEMEVKNQEKTVSLKDLDTEGYRNTTSTSLYS
                     APLTSKGRFLGTVTIGGPITQSGSVMEGNGRYLIETIANYISSGLENTLLTTKLKDVL
                     KELNSAQERLVEQETFRNLGEMTANIAHEIKNPLVIIGGFTKRLARKTRLDLTENRYI
                     EIILKEVARLEAILDEVLNYVKESPMVIKDSKISDFIDELVFLFTSDSAWEKIEITRE
                     YANNISFVPCDDQQIKQVLINILMNAYDAMQGSGKIVIQTGLKIINDRPFASISISDT
                     GGGIDPAIIDNIFNPFFTTKDGGTGLGLAISNKIVLNHGGYIQIHNLIGKGATFIVHL
                     PIKNTTIREERI"
     gene            25636..25998
                     /locus_tag="PK125_03070"
     CDS             25636..25998
                     /locus_tag="PK125_03070"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HPB37121.1"
                     /translation="MKKARLLIVDDEENIRFLFKEELEEEGYSVDVASNGLEAIDKVK
                     HAVFDLVVLDIKMPGMNGIEALNEIKNINKNLPVILCSAYGEFKQDFSSWASDGYVVK
                     SADTNELKQTIKNLLGVA"
     gene            26177..26251
                     /locus_tag="PK125_03075"
     tRNA            26177..26251
                     /locus_tag="PK125_03075"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:26209..26211,aa:Ala,seq:cgc)
     gene            26318..27628
                     /locus_tag="PK125_03080"
     CDS             26318..27628
                     /locus_tag="PK125_03080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB37122.1"
                     /translation="MKKLIVLLIALSVISCSTNPFAKKKTDSTGVPGSEGKKEQTQKT
                     AQIGEETPKPGDIKILDGVEYIYARNKRWQFTPYEPEYMWIRKDQYSPGWGENLLTRG
                     KPKEQKELESRLAKLEEEMKRKGLPPQMAYPSQMAYLPPGIAGYMPSVPLITFNYPSP
                     KMKRRVIVLPVADQTNYKDEQLGELATKRLISRLENTGTMICIDPNSVNMKGNLTDRQ
                     NLKALNETYGIQAVLKGTLADVFTSTSKIEGKDDRETSFAVSKIVIDLYNTDTGIVMK
                     QLSGRNPVFLSREKGDLSSEKAKIKALDLSIEIIADDILKSILSLDWHARIASIDKDK
                     IYINAGRLSGLEKGGAIEVYSPGEEIVDPKTNLPLGRTKGSYKGELEIVELFGVDASW
                     AKTKKGANFSATDLVYLKPQEAVNPSTVKSESTPAKKPDQPKKK"
     gene            complement(27757..28317)
                     /locus_tag="PK125_03085"
     CDS             complement(27757..28317)
                     /locus_tag="PK125_03085"
                     /inference="COORDINATES: protein motif:HMM:NF013053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HPB37123.1"
                     /translation="MKNNRVNRYKMAQKQDSVLIVIDVQDRLMPVIQGREAILENIIR
                     LIKFSQILDMPIVMTEQEKLGPTLSEIATQTENIVPIEKISFNCFLSSEFEERIKQLK
                     RKTLILVGAEAHICVAQTALHALSSFTVHVVKDAVGSRTAENRNIAIERMQQAGAIIT
                     STEMFIYEVLERAGTDRFKEVLQLVK"
     gene            complement(28633..29286)
                     /locus_tag="PK125_03090"
     CDS             complement(28633..29286)
                     /locus_tag="PK125_03090"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HPB37124.1"
                     /translation="MRTKIILADDHKIIREGLRSLLEKQDDMEVIAEADNGLTTVNLV
                     QKLHPDVVIMDIGMPEMNGIDATTKITTEFKGVQVIALSMHSDRRFVMQMLKAGATGY
                     LLKDSAFEELVTAIHTVMRRQHYLSQKITDVIVEEYLQNLPKNETNVFTVLTPREREV
                     LQLIAEGKSTKQIASVLNVSVKTIETHRQQIMDKLNIHSVAELTKYAIREGITSLES"
     gene            complement(29261..>31201)
                     /locus_tag="PK125_03095"
     CDS             complement(29261..>31201)
                     /locus_tag="PK125_03095"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HPB37125.1"
                     /translation="VKQSSDGVYIFDPETARILEANDQFTKLLGYSEDEIPGLSLYDI
                     VISRKEMIDASIRKVLRDGTYIYGLRHYRHKNGSVIDVEISGTLIHYGEAQVVMVNLR
                     NVTERRKAEEKLRNQAKQLKEQAELLDIAEDAIIVLDMEDRIVFWNAGAEERYGWTKD
                     EVKNQVSYAVLHTVFPEPLIDIKAKLAIVGRWEGELVQKKRSGEEIVVESRLALRRDQ
                     KNRPVAIMEINNDITERKQTQIALQRSKEELEARVAARTAELQDANDRLIVELNRRKR
                     IEDMLRKGAERYRNLFVNSPLGIYRVNAEGRILMGNPTLIRMLGYSSFDELVSVGGKR
                     RDYEPSYLRKKIKDRLEKEGRVRGFETKWRKRDKTSIFVRENAKAVRSPDGTLLYYEG
                     TVEDITEQKKAEEKIKSYQKQLRSLASDLSLAEERERRRIATMLHDHIGQILAISKIK
                     LGALAQLIGNDESMNQLKEVRNHIEQAIQYTRSLTFELSPPILYDLGLEAALEWLAEQ
                     VREHQRINCMFEADAYPKPVSDEIRIVLFSAVRELLMNVTKHAQANVAKITIRRVNDS
                     ITVHVADNGIGFVPSKMSFYRDENKGFGLFSIRERLRHLSGQMEVRSGRGRGTRVILT
                     APLVIEKQKAGNITNENQDYFG"
BASE COUNT         8769 a         6767 c         6497 g         9168 t
ORIGIN      
        1 actcttttct ttattcttct ctggcaataa tgtaccgaaa taaagatata aaggggtatg
       61 agaatgcctg tattgtatct gggcaccaca agatactctt gaaaggatgc tcaaagccat
      121 tctgacaacc attatggaat gtgctgaaac agtcaggact cctgtggggc tcacaggact
      181 attattttgt cattaacggg aaagggtaaa aagtggtacc aatcaaaata attgagtttt
      241 ttggtgctaa atgtgtcttc agaaggcaaa tatggtatgt ggtttgcaac gagacagtgc
      301 attatggaaa cgaaaagtga tattttatca ggaagatatg gtcatcaaaa aggcttcaca
      361 cttgtggagt tgatcattat tatcgttatt ctcggtattc tggcagctgt cgccattcca
      421 aaatatgctg atatgaaagc agaagcatca aactcaacgg ccagggcgat cttggggggc
      481 cttcgtgatg ccgcaacaat agtatatgcc aatcgaattg tgaatgcatc aaccgaacct
      541 attgatatga cagccgtttg gagccaggca caggtttctg gttatgatac ggatcctata
      601 atatcatcgg atacgctcac cctacagata tcagggcaac agtatgctta tcgtcttgta
      661 aaggctggcg gactggcgac tgggcaacca aatgtaatta tacagaatca accatcctgg
      721 taatgttgca gccagggcat agagcacaat agcgtggttt ttgggtatgt ttcttgcaaa
      781 ctgaatcctt acaaattgca tggagggcgc gtaaatgcgt acggtcagta gtgcaaaagg
      841 ttttactctt attgagctaa tcattataat cgttattctc ggtattctgg ctgctgtcgc
      901 cattccaaaa tatgttgata tgcgtcagca ggcagcagat tcttcagcac ggggtattct
      961 tggcgggctc aggggcgccg taactgtaat ttacgctgac agggctatta agagtcaaac
     1021 tacaggtatc gacatgacag ctgtgttaag tcaggtgcaa atatcagggt atgattcatc
     1081 ggttcctggg gcgaacacat tgcttataaa tatatcgggc aatcagtata cctactacct
     1141 caacaataca gataatattc ccacaacacc accatcggta acgatacaga cggggacagg
     1201 aaccccgaac tggtaaccgt cttcctgtgg aaaaaaataa tagccttttc tctcctccca
     1261 ctagaaacca ttgaaacaac gatatgacgc aggagaacag agaagtattt cacgattctc
     1321 gaaagcggta tactcctgca cattcaatga aaaaccaaca tctcgatatt acgttaaaaa
     1381 acctgataac tgtatctggt ctttttttta gtataagcct cctttatttt ttccccatgc
     1441 tgtcatcgaa aaatatcttt gttgaacgag accttgcagc gttctttata ccaccaagat
     1501 atctttgggt ctcattggtt aaatcttttc agattccgct ctggaaccca ttcaattatg
     1561 caggcatccc cctccttgct accttgcaac ccggcatctt ttacccaccc catctgctct
     1621 tttttatact accttttaac atagcgtgga actggatcat tatctctcat ttttttctca
     1681 ccgctgttac aacctattcc ttgttaagat atttgcgggc atctcatgaa ggggctatcc
     1741 tcggcgctat ttctgtaatg ctgtcaggct atttgatttc tgtgcacaat ctgcttaccc
     1801 atcttctggc agtggcgtgg tttccgctta tcttggtttt ttttatgcgt tactttgaag
     1861 aagggaaaaa gaagttcctc gtttttacct ctatccttct tgcaatccag ttctttgccg
     1921 gagcaccgga aatttccttt ataactgtta tggggctttc tgtaatcgct atctttatca
     1981 acaaattcat ggacatagtc ccccactttg tgtcgtcttc agcaactaga ttaaaagctt
     2041 tgggtttagt ttttagcctc ttttttcttc tttcatctgt tcagctcctt ccatttcttg
     2101 agcttaaggc ccatagtgtt cgggccacgg gcttaagtta tgtggaagca gttacatggt
     2161 cttttgcgtg gaaagacttc attcaatttt tcatacctga tgccttcgga tatttctcga
     2221 gtataaaaaa gtactggtct aaccaatcct ggcttctaac gctttattgg ggtattacgc
     2281 ccttcattct gtcctttttt tatttcattt cgaccgacaa aaaacgggtt gttttagcct
     2341 ctcttatgtt tatttctctt gtgttggctc ttggtggaaa cactccggct tataaacttc
     2401 tgtactacat accgccattc agtggaatcc ggtatccggt aaagtttatt ttcctattca
     2461 tttttttagt tgctctcaca gcaggacttg gatttgacta cttaaaaaaa ggtgtgccca
     2521 ggggagatgc tcgtgtcagg cggacaatac tggccttttt ttactcggga tttatctttg
     2581 ccatactctg gggattcatg agcattttcg gagatgcagt cacaagattc ttcgaatcag
     2641 cgggtttcat accggacaaa tataatgaag caaaaataaa tattcataat ataaaaagat
     2701 ttctcttgtt ttcgtttctc ttctgtgtct tgcttgtctt caacctgagg gttcgtttca
     2761 aaaaaactgt cctttttctt gttcttctca tagctgcact tgacatgtat tttggaaatg
     2821 ccggatatta tacagctgga aattggaaat tttatattga accaaagggg tggggcacct
     2881 acactttttt caccaaattg tccgacaaca aaaacacgga caggtatttc gttactttta
     2941 aaaccttaaa tgattttaac tatttccctt atgatagaac cattcttgcc ccaccctatg
     3001 cctctctgtt tggtctgtat acattcggcg gtgcagaagt tttgaggatt ggacattatg
     3061 agaattttgc aaacctgctt aaggattcac cgacgataga ttctggaaag aggtttcttg
     3121 atgtttctgg tatacgtcat gtgatcacca ctcaagaatt aacagacaac gacttcggcc
     3181 tcttacagag cattgatatg ggcagcagga cagcgcacct ttaccagtat ctgcgttatc
     3241 ccggacgatt catgctgttt gggaggatgc acgcagcaaa agacaacaaa gaagccattg
     3301 agaaacttct tgatcaatct attgatctga agaaagaatt ggtgattcaa tccacaaatg
     3361 ggtttatttt gaatgaatct gacgtaaaag gaagcataag actcctctca tatgaggcca
     3421 acagggtctc tcttgcctgc aaaactaaca gtgatgcatt cctttatgtc agcgacgtct
     3481 attatcctgg ctggaaggca tatattgatg aaaaagaagt atctatttat cgagctaatc
     3541 ttgccttcag agcagtatat gtgccaaaag gatcgcatac tgttttattc gtatatcgac
     3601 ccctttcctt ttatgtcggt ttttgcctca ctttatttgg ccttgttgcc tcctgttttc
     3661 tccttataaa aaaccaaggt cagaaaataa attatgttta gtcctgggag cacccgaggc
     3721 tttgggcttt ttgagaggtt tttggccaag aaaagggcgc aacttgcaga tagccttata
     3781 ccgcgaccat tcagaagtgg aaggattttg gatattggat gcggcatgaa tcctgccttt
     3841 ttacaaacaa cagctttctc agagaaatac ggtattgata aagctggttc tctctcatgc
     3901 cacactaaat ttgatgaaca attgataact tttattcatc acgacatcga gtcaggtgac
     3961 cccttcccct tttatgatga gttctgcgat gttgttacca tgcttgcagt gattgaacac
     4021 atatcgccaa ataatctttc aaaagtcatt gcggaattat acagggttct gaaacgtggt
     4081 ggccttctca ttatcaccac cccatcgcac tggacaggtt tcatcctgaa cggaatgtct
     4141 cttctacgac ttatcagcag acaggaggtc aaagaccaca aagaacttta tgcgtcggat
     4201 gacatcatga aggctctgca actggggggg tttgcaccag gaaacatgca gcagggctgc
     4261 tttgaatttt atatgaatac atggatcatg gccatcaagt gagcgctgaa tggttccgag
     4321 aaattctatt caaaataacc ttccgaagtg ttacaatatg ttattaaaaa acgttgcaag
     4381 ggggatttat ggaagaggtg gaaatcggta aggttttgaa gtatttctct aagattggcg
     4441 ttgctgcaat aagaataaca gatggcgaac taaagacagg agatacgatc aaaattaagg
     4501 ggcatgtgac tgactttgaa caggtcgttg aatctctgca gatagaacat gaaagcgttg
     4561 aaaaggttga agctggaaaa gatgtaggca ttaaagttgg aggggttgtg agagaacatg
     4621 acacggtata tgttgtaaaa agatagtttt tttgttagaa ctctttgagc aaacctcata
     4681 gccgcgtcta ttgcctgata ctatatatgc tacgcgtttt tatactatca ttcacagaag
     4741 atcgagggcg taatcgtttg gctggaccgc gccttaaagc gtgctgacat tggcacaggc
     4801 attggaacct acctgcaaca cagcgaggga gcaggtaagg ttcttgtagc ttgcttggca
     4861 aggctacaaa tgcttaccca agcaatagtg agctgttatc aaagaataat tttcggccct
     4921 tatctgattt aaggtgctga caacaagcat ttcgtatgct ataaaaagat aacagacaaa
     4981 taactgtgat catgaggagg aaaaatggaa gaacaagaac ttaccaacag attttgcagt
     5041 ctctttgaag aaaccttgcc tatattaacg aacatttaca aagggtttgc aggccaaaaa
     5101 attaatctcc ttaaagaaag cagatcaaaa tttagagaag tctggaaaaa ccggctcccg
     5161 gcagtagaga aactcattgg agacacggag aaaaatgagg tggagaaaaa atttgtggct
     5221 ctgcttccac atctccagcg tgttgctctg gcaattgaca atctagttga taaaatggaa
     5281 attaaagtgg agacacacat cttgttttct caaaaagcgc tggatgaaat aaagcaactc
     5341 atggtagccg ttggaacaga gttcactgat gtgaaggact attgcatgac gaaaaatcct
     5401 gtactgaaag aacagatacg tacggatatg gaaaagatca gaaaaatgat ttacgagttt
     5461 gaaatggtcc atcagaacag gttaattacg ggtgtgtgta tgcctaaagc atcatacctt
     5521 tatattgata tgactgattc cctgaaaagg atggcgaaag aactcggtga cttcgctgaa
     5581 aaggcatagc cttgtgggtg tcttcagaca accttactaa ggccatacac aaccaacacg
     5641 tactgaacaa aatatccaat tgttcttttg cagacaaagc ttttcttgta ggcggcgctg
     5701 taagagaatt gttgcttaac aaaacacctg ctgattatga tcttgttcta acgaggtatg
     5761 gtgatcttcg gatactcgaa aatgtctttg gtgtttctgc ttttctttta ggaaaaaaac
     5821 cgattcagac atacaggctc gttcaagaga atatttctat tgatattacg tttcatgcag
     5881 gggccatcga agaagacctg gaccgaaggg attttacgat gaatgccata gcctatgatt
     5941 tcaaagaacg atgcattgta gaccccttga agggtataca agatattgaa gcacgtatca
     6001 tcagataccc tcacaggaaa aaccttgaag acgaccctct caggatgcta aaggctgtac
     6061 ggcatttcac tatgcttgat ggcttcaaaa tggatgaaaa tcttttcaat gcaatatgta
     6121 ttctgaaaca tcttattcac aaaacagcac cagaaagaat caaatatgaa atggatcaga
     6181 taatcctttc ggaaaatgca ttccttggcc ttaagatgct tcaggaaacg ggcctccttt
     6241 ttgagctttt ccctgaacta tatgcattaa agaagctcga tgaagaaaaa cagtttattc
     6301 ttgagacttt tgggcacgct atagaaggat tcaaatattt aacaaaatat ggacgggcct
     6361 atgaactcga tgagagggac ttccgaaata caggttacgc ccttcttctg cacgatattg
     6421 ggaaggcaca caccttttct catgacgagc tcaaaaatgc tgttcacttt ttttatcatg
     6481 aaaaagtttc ctgcaacctt gcaaaccaca tcatggaaaa gttccggttc agttccattg
     6541 atattaaggc cattatcaag ctcatagaaa accatatgcg tatttttctt atcagtaaca
     6601 gtgactctac tgagaaagca ttaagaaggc tcgtttataa gatgggcgat ctcacacctg
     6661 cccttatcgt tcttaccctg tgtgacatgt atggaagctc tgctggaacc gaaaatgatt
     6721 caacttttat ggttcaaaaa aagtgtgctc acgtactaag ggcttataac gaatggaaaa
     6781 agaaaccact tccacggttg ataaacggta atgagctgaa agcaatcgga tttgaagagg
     6841 ggccaattat cggccaaata ctcaacgaaa ttcgcgaaaa acagatcaat gaagaaataa
     6901 cccttaaaga agaagcgata agctacgcca atgatcgcct caaaaaacaa caaatgcatt
     6961 aatcctttct tttttcaaca atttttatta gggtatccat gaagtcttgg ttatcaggag
     7021 acaattcgac agccctccgg gcatgcatct ctgcttctac aagattcctt cctgatatat
     7081 agttcaacca tgcgagatta ttatgtgccc tcgggtcgtc tgttttgctg acggcaactt
     7141 tatatgcctt ctcggcttca gcatagtcat tgtgctgaaa acatatattg ccaatataaa
     7201 gagatgctat ggggagcttc tttaatgcag ttttgtattc atttagagca gcatcatact
     7261 ccccgtttct ctcataactc aacccaagag taatatgctc ttcaggggta agaggatcac
     7321 ggagcacata aatttttggc attgagcagg aagcaaggaa aacaaaggaa atgagcagcc
     7381 ctatggcttg atggcaagag cccagaaatc ggtttttttc catatagtta taagtgattc
     7441 ttttaagatc agatggtttt cttctcttcc agagttgaaa atgatgccat cctgagtata
     7501 tccctttgcc accatgaaat ggttctgctg gtaaaaggct cggccgttat caacaagaat
     7561 tatgaccggt gaattttcat caatatattc tttgattttt tctatcgtcc ccgagaattg
     7621 cagagaggta aacccgagtt ttcgtgcgta cagctcaagg tccagattga gaaccccttt
     7681 tgctctgttg ctaaaaatct ctttggcaac tgcatcaacg ctgagccgct cattgcatct
     7741 gctttttctg tgccaatagt ttatgactgt aacaagggct gccgggccgc attgaaaatc
     7801 ttcttgctcg aaatatggga ccccttcgat aattctcgca tctgccgtta ttcgtcccgt
     7861 tgtagcacag cctgtcaggc agagcaagac gacaagcaaa cagagaaaca gacaattttt
     7921 cataaaaagg gttcgtgccc gggttttatt tattgattac attccggacc ttatccttat
     7981 agcctattta gttactatta ccttatgccc tgttgtaagg ttgataagaa tgatgatcag
     8041 aatgatgatc acaagcaccg caatgacaat gccaagaccg ctatcctgcc caaccctcaa
     8101 gctgtcgagt ttttgcgcaa tactatggag ttgctgatca cttaagaggg caagcttatc
     8161 cttaatctca tcgctcgaat agccgagatc cgccagcctc tgctggacaa gtctactctc
     8221 tagggctgat tgaatagcct taaggtcgga tcctctgtct gaatacatat aatttatggc
     8281 ctgagatgga acaaaggctg cttcaaggcg tggcgcaatg ccaagaataa acattgccga
     8341 tattaagcac catacaacac actttctggc catattctaa cctccacatt ttaggtatta
     8401 tataaggaaa gggcgctgga atgcaaacga tttttcccct acatgttcag ggcatttggt
     8461 ttgtcctcta ttgtcctcta ttgacaaaga caatataata cataaacttt tccatagttt
     8521 ttgtatgaca ttggcaagat atattccgat gataagacat ctattcttaa aggcagggat
     8581 ggccaatgaa caggagacaa ttccttaaag ccctttcaac atcctttggg attataggaa
     8641 tgagtggggg cttaaaacat cttttttcag atgaaccctc tgttctttct gttgcagagg
     8701 ggagtgacta tgcagccata acaagaagtg ccattgcagc gttaggaggg atgcagaaat
     8761 ttgtaaaaca gggaagcgtt gtagtggtca aaccaaattt agggtgggac agaaaaccag
     8821 agtacgctgc caatacccat ccccttgttg taaaaacgat tacagaagaa tgtctgaaag
     8881 cgggagcaat aaaggttaaa gtctttgaca atccatgcaa tgatccccgc agatgttatg
     8941 agaacagcgg tgtagccgat gctttgaaag gcataaagaa tgttcagctt aagcacatgg
     9001 aaagcgagcg ctataaaaat gtaaaattaa acggtgtttt tttaaaagca tgggagctgt
     9061 atgatgaagc cctcacagca gacgttttca ttaacgtacc cgtagcaaaa caccatggcc
     9121 taaccagggt gtctatcgga ttgaaaaaca tcatgggtat tatgggcggc aacagaggtt
     9181 tcatccaccg gagcatcgaa gacgccctta ccgatgtgaa tacggtaatt aaaagtcacc
     9241 tcaccattgt cgacgcaacg cgtatacttc tcaaccacgg tcctcaagga ggggacttaa
     9301 aggatgtaaa agcactgcac aaggttgttg cttgccggga tatcgttgca gctgatgcct
     9361 atgcaaccac attctttggt cttaaccctc agaacatttc aacaacagta actgcttaca
     9421 aaagaggcct gggagagatg aacctatcca gaatcaaagt aattaaagca taaatgaaaa
     9481 taacatggtc gagaatctca caagttctct ttctcgcgac cttttttgtg ctttttgtat
     9541 taacagaata tcgtggaaag gatattatct ccgctgctat taacagcttt ttcagggcag
     9601 acccccttgt tgccgtaagc tatctcctct ctcaaaaaac atttactgtg gttcttttgc
     9661 cgggtatcct gatggtgctg tttaccgtaa ttcttggccg attcttctgt ggctggctgt
     9721 gtcctcttgg cactatactc gatctcgtca cgcggtacat cccgaaaaaa aagcacatag
     9781 gttttctgaa atctcgcttc aaatattatc ttttgttctg ccttcttttt gcagcgtttt
     9841 tcaatgtaaa ccttatcggt atccttgatc ccatcgctat cgtagtaagg gcactgacct
     9901 tctttttcta tccccttttt ggttatgcgg tccggtcaac ctgggctgga ttatacaggc
     9961 ttattggaga ggacagggat cacgtttcct tcatattcgc cttttttcgt gatcatattc
    10021 ttccttttcg ggaaactttc tatcccttgg cttttctatc tttgatcctt tttgttgtaa
    10081 ttcttgtttt agagcattac gaaaaaagga actggtgcaa gaacatttgc cccttaggca
    10141 cgctcctcgg tctcctcggc aggttttccc cctttaaacg gctcctttcg agactttgtg
    10201 ttgattgtaa aaggtgcaag gatatctgtc ccacgggctt tgacgaagaa atcctgcaaa
    10261 aagaagattg cattctctgc atggagtgca agctcaaatg tgcatcaaag agggtaaggt
    10321 ttgtcctatt ccgacgatca aaagaaaggc ttggcagcac ttttgttatg gaaagaaggg
    10381 ttctcattgg cggacttcta tctggctttt tcatttcaaa agctttctta tttcagtcac
    10441 ctggcaggca tgagcggctt ttacgacctc caggcgtaaa taacgaggga gattttctta
    10501 agaaatgcgt caggtgtggc gagtgcatga aggtctgcct gaggagcgct ctttaccctg
    10561 acctttcaaa gggaggaatt tatgggcttt ttatgcctgt gctcatccca cgtttaggct
    10621 actgtgaata taattgcaat ctgtgcggtc aggtttgtcc aacgggcgca atacctaacc
    10681 tttccctgga acaaaaaaag aagagtatta tagggctcgc tgctatcgac aaaaaccact
    10741 gtctcccata tgcaaaaaaa atgaactgtc ttgtatgtga agagcactgt ccgattccag
    10801 gaaaagcaat tcatttcgaa actgtgacag atgtagatta cgcaggaaaa aaagtgattc
    10861 ttaaaaggcc ttatattgtt gatgagttgt gtaatggttg tggcatttgt gagaacagat
    10921 gtcccattga aggaaaatcc gctatagaag tgttttctgc aagaaaaata agcacatcca
    10981 agaaataatt aattttgtta caaaattata attaatatag acaaaacgtg tagttttata
    11041 gtataatccg ccgtttattt aaaaaggagg tgatgctgtt atggtatgtg aaacattaaa
    11101 accaggggtt gagtgccttt ttatgaagaa gaccggatgt tcatacaatg gtggtaaatg
    11161 ctatactatt gttgagaatt gcgaaggttg ctcaaaggtt gtggaatttg aaaccggaaa
    11221 atactgttca agttttccat atcctgcaca aaaatggcaa aagggttctt gctccatgtc
    11281 ttcccacacc aagaagtcgt cacaggcagc agggcaaaag attaaccctt tgaaggcttc
    11341 caagagatca gccggaaaaa ggtaaacttt tcactgtcaa agttgatctg gaataaaggg
    11401 cctatcgttt tacagtttat aatgaacatg gatgattcag agggatttat cttatatggt
    11461 tatatcccct atcaaacaca acggacgctt aaggcccttg atctccagcc tcaagcgttg
    11521 ttgcataaat aatctatttt atcgtttgtc gtctgctcgc caaaacaaaa atgcgagaga
    11581 ggggatttga acccctacgg ttacccgctg gatcctaagt ccagtgcgtc tgccagttcc
    11641 gccactctcg catgcctgat tttattatgt ttttgctttc catgtcaatt ctcctgggct
    11701 ctaaatgcat taaaattgta gcaatagccc aaatcttcga catttcatcg gaagctttac
    11761 gggccacggt accacaccgc acgctatatc ctatcttttc ggtgccctga tagccatggc
    11821 cagtttatca atcctctttg catccgaatc tctgcccgtt ttcctgtaca gcactgccat
    11881 gtcaccaaga attggtgcta tgtcagggtg gttgggcccg agggttctct ccatgatggc
    11941 aagagcgcgt tcgtaagtcc gctcagcctt gccgtagtgg cattgagcaa gatacaaccc
    12001 tgccagtttg ttcagcccca gcgcgtactg gggagagtct ggcccatagg ttcggtacag
    12061 atcagcgaca acgataagat ggtcttcaag agatgggcgg gtaatgggaa ccttctcaaa
    12121 ttgtgtggtg tcttcaggct ggacctcgcc ctcaagagta aaatagaata gaaagttctc
    12181 aaatgcttgg tatagtgtct ttgcccggag atagggataa aaggccacta tgtctcctgc
    12241 gtattgtctc agggtgcttc gatcgacact cgttgctagc cgacatgcta tgttcacgct
    12301 tcctgtgtac ctgctgatca catttacagc acatagttgc atcagagtta atgacgataa
    12361 ccattttctt ttttcttctt ttttttcagc agacattggt tgaccaaaca cggcccacca
    12421 cctcatcatg caatcctggc cagctgcagg acacctgaag gtccagtcat tctcttcgtc
    12481 aaggcctttt gcgatcaaat actcgttttc atccctggga gtgtttagaa agaagtcaat
    12541 cagtgtatag tgaaaagcag cgatagactc gggattcgat ggagtttccc catactgctc
    12601 aaggttgctc accaacctgt cagcaagtaa ttggtgggat gttatcaccg gtgcgttcat
    12661 gggggtcagc acaggttcat cgtccttgta aatccagtac catctatcct tcttcactga
    12721 cctgtacatt tgaaggccta atgtgacagt tctgtctcct tttaaatatt acacccacgg
    12781 aaaaggaagg gcaaggggtt ttgtggcttg ttcgttgaat aggttttgtt ccaatgaatc
    12841 tacacggtgg catgaaacag aaaaacaaag tatggcacaa aataatcagc cctgccagaa
    12901 gcactctcag ggtagtctag tatttatgaa gcaacagcgc caaagtttta gagatattta
    12961 attgaaatga tgtgaatctt cattgctatg gtaagttttc tatctgaacg gctaccccaa
    13021 gcatcgtata gtttacgcaa aaatattaat tctgattgct ctgttgagaa aattggcaaa
    13081 atcgtatctg gtttaataag tcacatggac ctaggcttga tttaaggcaa gtataaccaa
    13141 tgtttctttt cataaatcct ttattttggc aaaaacggta ggttatacca ataggctgaa
    13201 tcgaccagca tggggtgctt atttcacggc atatttttgc tgcccttctc aactatagtt
    13261 acaaagtccc tcaaaacctt ccggtagtca ctcccagctg cttcaaaaag attgttgtgt
    13321 ccagcacctt ccacccaaaa ggacagtctt ggctcattgg cctcttcaaa cagccgctca
    13381 ccatgccaga atgggaccac ttcatcctgt ttaccatgga taaccaaaac cgggcaacga
    13441 acttttttca ttttcgatat gtttttgaat ttatcaaatg gaagtagggg tatccgcgtc
    13501 agtacccgat aagctgtaac gaatgagctt tcaatgatca acccgcctaa ctgcctcttg
    13561 tgagccaggt ctatcgcaac agcaccaccc agcgagcgac cgagtgcaat aatccgacaa
    13621 gctgggatac caagattgtt caccagatag tcataggctg tctcctcatc ttcgtacacc
    13681 ttcttttcgg atggcgtacc caagcttgtg ccgtatccgc ggtaatcgta tgcgaacaag
    13741 gaaaagccca taccttttat ttcctccaac atgggaagaa gatggccaat gtcttcggca
    13801 tttccatgac tgtacaaaat tgtgtaaaaa gcattgggat tacgtagata aatagctgag
    13861 acagcctccc cgttaccgga atccagtttg atgatttgat ctgagtcctg ataagaggag
    13921 ggttgaggct gaaatatcat tgtctcagaa acaaggcacg aatagagaac cgtgccaagg
    13981 tatacgaaaa ccataatgcg acatactgag actaccaatc gcttgaggcc gatgccacct
    14041 cgtttcgact cgtgtttatt cacggcctat cgtgttagcg tgttccatgt cacttgatat
    14101 aatagcctgg aaccaccatg cactactctc gtccatcatg gggaacgctg tcttcattgt
    14161 ggacttcctg ttggcaaaca atatgccgaa cttgatcacc acgtattccc cgttatgagc
    14221 gccaggtggt gatgtccgat acgtcttttc catacccttg cgagtcaata cattgcagag
    14281 atgtctatga gttccttcca ctacctgttt ccattgtttt cgtattagag ggaggcatat
    14341 ctaagaacac tgcatcccac tcccagccct cagcgtattt tttagccaac acaggccatt
    14401 tttctgctac catagtcacg gcctcttttc cagactacct gttaaggcgg tggtcagaat
    14461 acgaaccaga tttgtggtgt ctggtattcc cttaacttga acaatatatt tacttaaata
    14521 atattacact catcaacggg gaacaagaaa aatttgtaac tgtggtggcc cgtacatggc
    14581 ctatcaatac ccttgaaatc gttagtttct ctgttattgc ctactgtagg ttcacattta
    14641 ccccaagaga actagacggg tttcgtttaa aagcacaata tcagcaacat gaatgttgtc
    14701 tagtgcgctt gcttaatgtg tatactcagg caaaaattcc agccaaccaa tatggagttg
    14761 ccaggctcaa catatctatc tatttccttt tgtctacaga agcgtaaacg aagcatatcc
    14821 ctatgaatct tgggctgggg cattttcgac ttcgcaagat tccgggtact ggtgctcgag
    14881 aaataatggt tcaccattcc ttggtgtgtc aattggcaac gatgttgtat ttccgtgctg
    14941 tgcggttgat accttcaagg gttgatgtag tgtatatgca ggttgatcat aaccacgatt
    15001 caaaaactcc gccgtgaccc gaaaagagta tattccacaa atgagggtag ttactcactc
    15061 ctcactgaaa ttattttgga accgatgttt tagacaagtc ccatgctcag aagggctgag
    15121 atttccttct tgcttgcctt cagtacgcgg tatcggtgag agaaccatga ggatttgtcc
    15181 agcgctctcc cattctctag gaggataacg ggctgtccat atcgacttaa cggatgattt
    15241 gcaacaatgg ttgccttgac acggtgctgc atgtttcgtt tatcgaggat gtctatgtgg
    15301 gacagaatca tactgcaacc tcacgggaag aacacctcta tgaagcaacc ccaggcatcg
    15361 ttggtgatga tacaaagttg gtcaagcacg gtgcccagag tctttgaggc ttacatctat
    15421 attttcgtcc actttcaaac atttattaat aaaggccaca aaatgtgtag gacacatcat
    15481 gtaatttgtg gtgaaacctg gggccatcag ttcaatgttt tctgaagaat caaagcatga
    15541 taaggtcctg atattaaaac aggtttttca tcaatactgt tttcttaccg tcagtggcac
    15601 aatatatgct gaagaggggt tatgtacggt gaggagggtc atatggaaag tatggaatta
    15661 atgctggttg aacagccaaa acgagaagtt ctcgaaaaag acgctgtttc ggtttttcca
    15721 ttcttttttc ccggtatggg gaataggctt atgggactca gaaacaatgg gaaaaaggct
    15781 tatattgtgg ttgacctttc aggcagggta tttttttatg accgggaagt ttcatcatcc
    15841 aattagctgg atgataattt aacgcggagg ttgcactgtg agacaacaga acaaagtaag
    15901 agaagtgatc cgtatattga tggagagtag attttacttt accttgtctg ttcgggaaag
    15961 gtatgatctc ataagggata tactgaggaa gttccctttt tcctcatagt tcccatacct
    16021 tgaaaaaggg gttgcctgca atttcttgtc ttcttcgaaa aacttttcat ctgtaatcat
    16081 ggtaagtggc tgtaggggaa cagaccttat atggaaggtt ttatttaatt ctcagatctt
    16141 tccaggcctt gtttctatat ctgatttaaa ccgaggcact tgtgcgtttt tcctttatac
    16201 acgtattagt gaaaaatgta tgttccttgc atatattctg ctgagaacaa cgcaaccaaa
    16261 aatgataata tttaacctgt taataaacga cataaggagg gggccatgcc atatgtgaac
    16321 attaaagtaa caaaggaagg cgttaccgct gagcagaagg ccagactaat cgctggggta
    16381 actgaccttc ttaggacagt gctgggtaag aatcctgcta ctacctttgt catcattgac
    16441 gaggtggata cagataattg ggggattgca ggagagcagg tcactttaag acgtagcaga
    16501 gggatgtaaa caaaaaccct ctttacgcat tgtatgcact acctggcttg gtagattgca
    16561 cacatggcaa tcacgataat gagaaaaggt gacattctgc tcggtgatat cacgaacaac
    16621 gctcagcgca atgtctgtat tcctttgaag gagttgtggt ctcgtctcac cggttcgcta
    16681 ggctgtgccg gctgtgatga caataatctg tgagcttgtt tggcctgatg catctccccg
    16741 catgaatgaa ggttgtgggg gaagacggtt gcccgtgtat aaggtcggcc ttcctatcat
    16801 tcatgagcac aatctcatca actaaaccgc tcagtgcacg tgtatagcac attatgtaga
    16861 gccagatgtt ccagccccaa ctgctgtagt tttttgatgt tttccagatg agttcgtttg
    16921 taaactttac ttctatgtgt tctgcttatt gaaattccta cttatttact acttttcata
    16981 gacaatggcc tctacagggc atgcctcggc agcttcgatg attttcttgt ccaatgaaag
    17041 attagcgcct ttttttacaa cagcaacttc accgttgagt tcaaacactt ccgggcatat
    17101 ttccacacat gtgccacagc caatacaaga atcttcaatg aacacctgtt tcatttcaca
    17161 cctctacatt ttttctaaat attcctttaa tactactgcg ttgttgatgt gtggttcttt
    17221 tgcgccatag agaagggtga tgtgggtctc tttcatcttt ttctttaggg gttcgagcaa
    17281 ctctttctgg agagctattt cggcaaaata tctttccttg aacccggtcc atttctgcgg
    17341 ctcatggttg aaccattttc gcagctcgtt gcttggcgca atgtctctca accaaaggtc
    17401 gacatgtgct ttttccttgc tcataccccg tggccacaac ctgtccacta gaacccggta
    17461 cccatcatcc ttagaaggag gatcatagat tctctttatt gttatcatgg ccgtgtctcc
    17521 tctctgcttt tttataaatc cagcgaattg acgctgtctt tctctttcca ttttcagcct
    17581 ttctttggat tcctcgtatc atcttctcca caagtcaatg ttgaatcatc tgtgaacccg
    17641 ctggctatgt acgtgatctc ttcaacttct gatttacagc taccacataa tactgtgacg
    17701 tctgtgcgca taagtcaacc tcattgatta ctattataag gtggaacgaa taaaataaag
    17761 aacccccggt gttcggctgg tgttacaaga tgtggagtta agaattagct gaaatctctg
    17821 atcggaacat ccctttccag gatacccagt acgccttcca gccccttctt tgctgccaca
    17881 acagaatgct gacaattgat ccccttgatt actgctcttg tttctgccac ggcaaccccc
    17941 tccctcactt tttcatattc ttagggagtc aggcccccag agatcatcca gggcctgact
    18001 ctgtgatgat tattgccaca tggggcagta acctctcccg taaccaggac cgtaaccatt
    18061 gccccaaccc atgtgatggc ctctaccata accaggaccc cagccacctc ggtagtatcc
    18121 gcggccatag ccccaatttg acaggccgtt tttcttggct acatcactga tcttcgttct
    18181 cagatcaatc atctctttct gaagattggc aatctttgcc tgatccggcg tttctttgtt
    18241 gaattcattc ctgatttcca gtctcttaac agccatctca tctcgcagag gcaatgtctc
    18301 cttctggaaa tttctaaaag cattcacatc cgtttgttca ccagggttaa cccacggtcc
    18361 tctattccac gcagcgaata ctgccgtggt cagagttaag ccaaatacca caacaaaact
    18421 gaataccatt aattttttca tccctttttc ctcctttcaa tgggatttgt tactgatact
    18481 ataaggaacc agtgtgaaga ggtgacgaac taattatgaa aaattgtgaa tcccttccat
    18541 cgcaaagagc agtctatgtc tgctccaaca gacatagcgt ctcatgaatt attttatagt
    18601 gtctaaatcc cgtactcccc ttgataaggt cagcgaatgt gaaccgttac aatggaaaag
    18661 aagagtttgt aagcacgctt cacaatatac ctttatcttt tgtacgcgtt gtcgcaaatg
    18721 ttttttcacg tttaatttat cttgatagat ctgtattgtt tcgctatcgt ctaattatga
    18781 gcaccatgca aagtggcgtt ttatcataag gttttcccat gacaaaacac aatgcataac
    18841 tgcactggtt gaagtaagca tgaatttttt cataaagaac cccggaaacc gaagaccgcc
    18901 cacgtaatcc ggtaaggtgc aaaaggtaga ggttcctggt ttgtagatct taccgtgaaa
    18961 tcttccgagg tacacgtcct ctgcaacaaa aacctccata ttcacccttg ttcgtgttct
    19021 tgccttcttt gctcgttagt cctgaaacca ttgccatgtt tacagtcaca tcagtggtat
    19081 cggtagcatg agatttttgc ctttagccag gcttacactg gtcagactgc ttttcaaaaa
    19141 aagtttacaa caacccctgg tggtataaat atcaaatttg cccttggaac gattttttga
    19201 gggtcatgaa tacatttact caggaggatt cgtatttgtc catcctggcg aatcctcaga
    19261 aggcctttct aaatatgttg aaattccacc tgcaatctat ttattgaaac agaatttgct
    19321 accctgatct gttagctgct attctttatt tgtgtgttca ggaaaccttt tgtctctggc
    19381 atacagtttt aaatattcta gatctttctc gctgaaaccc tcccacgcaa atgggaaaag
    19441 tgcgttgttc tggtaaatgg gatcctccca aattgggaag cgcgcgcact gtccatactc
    19501 atcaaaaaga cgtgtgtggg gtatgggcgt atattcagct atatatggtt gtatgccgat
    19561 accaagaaca tagtcaatcg actgcttcac ctcttcccat ctttgattcg gtaagccagc
    19621 gagaatatat gcaacaatct cattgttttt gaatcctcca tcaattaagg cagtgacaga
    19681 tttttcaaaa tgctttagac tgatcttact ccctgttgac ttctgtacaa ctgggtcggc
    19741 tgactcaagc cctatcctta tttccctaaa tccggcacct cttaaaagat gggccagctc
    19801 atcatcaatg agggcaccgt tcatggcatt agggttatag atatcaattt gaaaagggag
    19861 actgcagatc ttcttcagga gaggctttgc ataaacctca tttttgtaaa gaaagttgtc
    19921 gtcataaagc acgtacctgc ttacccccag ttcgtgccaa tgtctgactt cctcaatgac
    19981 gctcccggga cttctcctca cgataccggg gtgcatatag ggtgtggcgc agtaggcgca
    20041 tcggtacatg catccgaatg atgtaagcag aggaacaaaa gggatggttt tataaagatc
    20101 ataacacggg taggggatgt gatcaaggtc atagagtgag ggtttgtagg tcaatttttt
    20161 atcaaatgca ttttcaacaa aaccatagaa tttttcaatc tcactgtttt ttatgataag
    20221 gtcagcttct cggagagtgc ggtcagcgtg tgcggagcag agagagggat atattccacc
    20281 aataattatt tttgaagatg gaaaaacatg tctggccgcc tctaatgcct ctttcacacc
    20341 aagataccag tacgtcatga tggaggtaat aaggataatg tccggttctt tactttcacc
    20401 aagctttctg atgagggcat ccctggagat gccatatcgt ttgaagcgtt ttcgaatgtg
    20461 tttaagtgcc ggggggctct ccaccttttc ttttacaaaa ggcgcccttc cgtccacttt
    20521 ccgttttctc tccacaattt gcatacagtc gatgaggtta acctccatat catttgctcg
    20581 gagtattcct ccaatgtaaa ggagtccaag aggacttgac caaaaactgt acgctgcgaa
    20641 atcatagatg gaaggattta taagcaaggc tcttgaaacc atttgtaacc tatttccctt
    20701 ttcaccctcg tttttttgaa ccgcggaaca aaggtttacg actgaaaagc tttatcatga
    20761 taagaccgaa gacaaaccct cctatgtggg cataccaggc tacaccttcc atgcctgaat
    20821 acaagacttg catgaaaaac caaatagtaa gaagcacaat cgctggtaat tcgactattt
    20881 tgataaaaat aataataaag agcaatgtct tgattctcgc ataaggataa agcaccaaat
    20941 atgcccctaa aacccctgaa acagctccgc tcgctcctat catgggaata tttgatgtgg
    21001 gatcataaaa gagctgggca aaagctgcgg caataccggc cagaagataa aataaaatga
    21061 atcgcccgtg tcccgtggcg tcttcaatgt tgttgccaaa aatccacaga tacagcatat
    21121 tccctccgag gtggaaaaaa cctccatgga ggaacatgga ggtaaagatt gtcatcacgt
    21181 tgtaaggtac aaggtccaac ctagaggaag ctgaggtcat aaattccctc ggtacaagtc
    21241 cgtaatattt gtatacaatt tggccttcag taccggtaag ggtaaatctc atccaaagaa
    21301 atattaatat gtttacaaag atgatgctca ctgttataat gggataagtg cgtgttgcaa
    21361 tattatctcg tatcggtatc atttgtggta tttcttatca cgtgtttctt ggcatgtcaa
    21421 aatcaactgg cctggagtga tgtatgcctg aattaagaaa ggatcctatt attgacagat
    21481 gggtgattat atcaacagaa cgaggtaagc gccctgtctt ctttattgaa gaaccgcccc
    21541 cgccaaaggc agctatgtgt ccgctgtgcc cgggcaatga acatatgacc cctgcggaag
    21601 tatacgcagt ccgaccaggc aattcatcac caaacagccc tgactggctt ttgagggtca
    21661 ttccaaacaa gtttcctgcc ttgcgcattg agggctccct caacaaagaa ggtgttggcc
    21721 tctatgacag gatgaacggc atcggtgccc atgaagtcat aattgacaca cctgtacatg
    21781 gagaaacgct ctctaatatg agtgttcgca gcatacagaa tatatttggc gctttcaaag
    21841 aaagggttct tgaccttgca aaggataagc gtttcagatt tactattgta ttcaagaatc
    21901 atggtaccat tgctggggcc tcccttgatc attcacactc acagctcatt tcccttcctg
    21961 tagttcccaa aagaatatct gaggagatta gtggtagcat gaattactac agacagaaag
    22021 accggtgcat tttttgcgat atcattgccc aagagcagga agataatgta cgggtaatct
    22081 acgagaacaa acggtttatt tctctctcac cctatgcatc gcgctttcct tttgaaagct
    22141 ggattcttcc caaaaaccat gagtcgcatt ttgtttttcc aagcgatgat gacggttacc
    22201 gtgatgcagc cgatgctctc tctatggtct tgaaaaaaca agacaaagta ctgaattctc
    22261 cgccctataa ttttatgata cacactgcac cgcttggaag tggcgatatg ccatactatc
    22321 actggcacat tgaaatcatc ccgaggctta ctaaaatggc cggttttgag tggggaactg
    22381 gtttttacat caaccctact ccgccagaag aggcaacaac gtatcttaag gaagcagata
    22441 tatagggggc ctaatgaaga tactgatagc gtctcccgaa ctttaccctt ttgtaaaaac
    22501 tggggggctt gccgacgtta caggaacact accgaaaacc ttgaaaaagc tcggggctga
    22561 ggtgagggtt atattaccaa aacacaaggg agttgaagag ctcaattttc ccctgcatta
    22621 taagaacttc aagatagcgt gccagatatc gcaggcctac gttgacgggg aaatagtgga
    22681 gagtgagtat gatggagttg ctgcatacct tgtggaaaaa gatgaatatt tctatagaga
    22741 ctatctctac agcaccccgg atggtgatta tcttgacaat gctgagcggt ttatcttctt
    22801 ctctaaaagc atcctggaag caataaaaat aaccggattt gttcctgatg ttctccattg
    22861 taacgattgg gagacagcac ttgtcccttt ctttctgaaa acgctgtata aagatgatcc
    22921 ccatcttaaa gatattgcca cactcctaac cttgcacaac cttggatacc aaggcctgtt
    22981 ctggcatcat gatatgcacc ttctcaacac gggttgggaa tacttcaccc ccgactacat
    23041 agagtttttt ggcaagatca attttctgaa agctggaatc gtcttcgcag atatattgaa
    23101 cacggtaagc aaacaataca gcgaagagat acaaacacca gaatttggtc atgggctgga
    23161 tggaatactc agaacaagga aagctgatct ttacgggatt atcaatggca tagattatga
    23221 cgagtggaat ccagaaaatg acaggtattt acccgccaac tatagtgctg atgatatgag
    23281 taataagtca cattgcaaga aggcattgca aaagaccttc ggtttacccg tgaatcaaaa
    23341 tattccactc cttgccacaa tctcaagatt ggcagatcag aaaggctttg acttaataca
    23401 ttatccatag aagatatgct cactcttaac gggggcttgc agtatattgt ccttggaaca
    23461 ggcgacagaa agtatcacga catctttact gatgttgcaa aagaattccc aaaatcattc
    23521 gccatcaaaa ttgcttatga caatgccctt gcccatctca tagagggtgg agctgacatg
    23581 tttcttatgc cttcccggta cgaaccgtgc ggtctcaacc agctatacag cctgagatat
    23641 ggaacggtac cgattgtacg cggagtgggc ggtcttgaag atactattac agattttgag
    23701 gtcaaccctg agctcggcac tggttttaaa ttttatgact attctaggac aagccttctc
    23761 aacaccatag aaaaggcctt aaaggtatat cgaagaaaaa aagactgggc aacgcttatg
    23821 aagcagtgta tggcagcaga tttttcttgg gaaagatcgg ccaaagaata tctgaatctt
    23881 tacagaaaag ccattgaaaa gcatgcatcc gattgatatt cttggaaaaa taattgcaat
    23941 atcacactcg aaccttgaaa tagcgtcaag gattaacgca atcctcaaca taattactca
    24001 ggacatgccc tttgatgaag caattgtata caccctcgat actgataaaa aactcacctg
    24061 ccggtacaaa aatgacaaaa gcgtcctttt ccctctcttg aatgagtacc ggtctcacat
    24121 cggtgaaggc attgtgggaa gtgtggctca aaaaaggata cctcagggct tcactttcaa
    24181 agatgtgccc cccagacttg gatgcctgtt ttatccagat cttgacggat atattcaaaa
    24241 atacagggct tttcatttta ttcctataag tgacgacagc tatctttacg gtgtcttatt
    24301 tatatgcgca tcgagcatgg agcgtctgaa caaaccccat aatgcttttc tgtccatggt
    24361 tgcgagagaa attggtggta ttctccgttc caaccagctt cttatttcat caaaaaaacg
    24421 cataagcgag cttgcaaccc tgtcagaact tggtagggtt ctagcctcta gttcaattcc
    24481 ccaggacatc ctcaacagta tcacgtttat cattgccaaa gcattaaatg ccgcttttgt
    24541 tactgtaagg ttgtcccatt catttcttaa gattgattca cagcggttta cctacggaga
    24601 aattggaagc gaatctaaag aaataatcaa aaaactcgaa atggaggtca aaaaccaaga
    24661 gaaaacagtc tctctaaagg acctcgatac agaagggtat aggaacacca catcaacgtc
    24721 cctgtatagc gctcccctca cctcaaaagg gcgattttta ggaacggtaa cgataggtgg
    24781 gccgattacg cagtcagggt cggtcatgga agggaatggt cgttatctta tagagacgat
    24841 cgctaattat atctcaagcg gcctcgagaa cacccttctt actacgaaac tcaaggacgt
    24901 gctaaaggag ctgaatagtg ctcaggagag acttgtcgag caggagacat tccgaaacct
    24961 tggtgaaatg acagctaaca tagctcatga aattaagaat ccactcgtaa ttattggtgg
    25021 ttttacaaaa cgacttgcga ggaagacccg acttgatctt actgagaata gatatataga
    25081 aataatactt aaagaggtgg ccaggctcga ggcgattctt gatgaggtcc taaactatgt
    25141 taaggaaagt cccatggtta taaaggacag caagatctct gattttatag atgagcttgt
    25201 ctttcttttt acttcagata gtgcatggga aaaaattgaa ataacaagag aatatgccaa
    25261 caatatttct tttgttcctt gtgacgacca gcaaataaag caggtactca taaacatttt
    25321 gatgaacgcc tatgatgcaa tgcaaggcag cggaaagatt gttatccaga ccggtctcaa
    25381 gattatcaat gatcgccctt tcgcctcaat tagcataagc gatactggag gaggcattga
    25441 tccagctata attgataata tttttaatcc attctttacc accaaggatg ggggaaccgg
    25501 tttaggtctt gccatatcaa ataaaatagt cctcaaccat ggaggttata tacagattca
    25561 taatcttata ggaaaaggtg ctacgtttat tgtgcatctt cccataaaaa acactacaat
    25621 tcgagaggag cgtatatgaa gaaggcacgc cttttaatag ttgacgacga ggaaaatatc
    25681 cggtttctgt tcaaagaaga gctcgaagaa gaaggatact cggtcgatgt agcctcaaac
    25741 ggattagaag caatcgacaa agttaaacat gctgtttttg atcttgttgt tcttgatatc
    25801 aaaatgccgg ggatgaacgg aatagaggct ctcaatgaaa tcaagaacat taacaagaac
    25861 ttgcctgtta tcctgtgttc tgcctatgga gaattcaagc aggatttctc gagctgggca
    25921 tcagacggat atgttgttaa atctgctgac accaacgagc tcaagcaaac aattaagaat
    25981 cttctgggag tcgcctgaca cttagacaaa tttgatgtgt ccaaaaggtt tattagagaa
    26041 caggtataat aacggataat tacagtacca aatctatcat accaaaacat ttgattctcc
    26101 aacactgtac aaagtatata ctttttcgac aaaatgcttg ctatagattt catgttgccg
    26161 taatattata cgggttgggg gtgtagctca gcgggagagc atctcgttcg caacgagggg
    26221 gccgcgggtt caattcccgc cacctccacc aaaaaatgat cgtttgacat accaccgaag
    26281 gtttgacttt gcacaaacaa ttacattata atcacgtatg aaaaaattaa tcgtactact
    26341 cattgcctta tcagttatat cgtgcagcac aaaccctttt gccaagaaaa aaacggacag
    26401 cactggtgtt cctggctcag aaggaaaaaa ggaacagaca caaaaaacag cacagatagg
    26461 ggaggaaacc ccaaaacctg gagatattaa aattcttgat ggagtggaat atatttacgc
    26521 aagaaacaaa aggtggcagt ttacccctta cgagccggag tatatgtgga tcaggaagga
    26581 ccagtactcg ccgggatggg gcgagaacct gttgacaaga ggcaaaccga aggaacagaa
    26641 ggagctcgaa agcaggctgg ccaagctaga agaagaaatg aagagaaaag gactaccccc
    26701 acagatggcg tatccgtcac agatggctta tttgccacct ggcatcgcag gctatatgcc
    26761 ttcagtacct ctgataacat tcaattatcc atctccaaaa atgaagcgac gtgttatcgt
    26821 attgcctgta gcagaccaga caaactacaa agacgagcaa ttgggcgaac tggcaacaaa
    26881 aagactgatt tcaaggcttg aaaataccgg cacaatgatt tgtattgatc caaattcggt
    26941 aaacatgaag ggaaacctta cggatagaca aaatttaaag gcattgaacg aaacatatgg
    27001 gattcaggct gtacttaaag gcacccttgc tgatgttttt acaagcacat ccaaaataga
    27061 aggtaaggat gaccgggaaa catcatttgc tgtttccaag atagtaattg atctttacaa
    27121 cactgacact ggtattgtta tgaagcaact ctctggccga aacccggtct ttctttccag
    27181 agaaaaggga gatttaagct ctgaaaaagc aaagataaaa gccctggatt tatccataga
    27241 gataattgct gatgatatcc tcaaaagcat tctttcactc gactggcatg cacggatagc
    27301 ctccatcgat aaagacaaga tttatataaa tgccggtcgc ctgtcaggcc tagagaaggg
    27361 cggtgctatt gaggtatact ctcctggaga ggaaattgtt gatcccaaaa caaatttgcc
    27421 ccttggcagg acaaaaggta gctataaagg agagcttgag attgtagaac tgttcggcgt
    27481 tgatgccagt tgggcaaaaa ctaagaaggg cgctaatttt tcagctactg acctggtcta
    27541 cttaaagcca caggaagctg ttaatccaag tactgttaaa agtgaaagta ccccggctaa
    27601 gaaacctgat caaccgaaaa agaaataatg ggtcccgcag gtcatcgggt aagagatgtt
    27661 taaaccgttt cacgtgaaac accattttta cttttaaatt ccaattacaa aagacaataa
    27721 atcctccggt ctttgtcata tctttttcaa tgctttctat ttaaccagct ggagcacctc
    27781 tttaaatctg tcagtgcctg ccctctccag gacttcgtaa ataaacattt ctgtacttgt
    27841 aattattgcc cctgcttgtt gcatcctttc gatggctatg tttcggtttt ctgctgttct
    27901 agaacctaca gcgtctttga caacatgaac ggtgaatgac gacaatgcat gtaatgctgt
    27961 ttgtgctaca cagatgtgtg cctctgcgcc cacaagaatt agtgtttttc gttttaattg
    28021 tttaatgcgc tcctcaaatt ccgaggataa aaaacagtta aaggaaattt tttcaatagg
    28081 aactatattc tcagtttgtg ttgcaatttc tgatagtgta gggccaagtt tctcttgttc
    28141 agtcatgact attggcatgt ctagtatttg tgagaacttt atgagacgaa tgatattttc
    28201 tagaatggct tcccttcctt gaatcaccgg catcagtcgg tcctgcacat caatcacgat
    28261 gagaacagag tcctgttttt gcgccatttt atacctgtta actctattat ttttcatata
    28321 tactcctctc ctttttagac ttgtttattt tccaaaaggt cgtgaaaaac ctactattcg
    28381 tcaccatctc atctcgatga atgttgcctc tttgtcaaat atcccattcc acgaagatag
    28441 ctctccacaa taggaattgc tgctgactca cgcctcctct taacatctgt caggtaacac
    28501 ggtatgggtg gcaacttaat aaaacgtgca tggacaggta tacaatccag aaacagatgt
    28561 aaaaaactga cacatagcga aacatcttct tcaaactaca tagctttaac gaacaataaa
    28621 tctgcctatc ttttaggatt ccagggatgt tatgccttcg cggattgcat attttgtcaa
    28681 ttctgcaacg ctgtgtatgt tcagtttatc cattatttgt tggcggtggg tttctattgt
    28741 tttaacgctc acatttaaga ccgatgctat ttgctttgtg cttttacctt cagcaataag
    28801 ttgcaatacc tcacgttccc tcggagtcag caccgtaaat acattagtct cattcttcgg
    28861 caagttttgc aaatactctt caactataac atctgtaatc ttctgactaa ggtaatgttg
    28921 cctccgcata acagtgtgaa ttgccgtcac gagttcctca aatgcgctgt cctttaaaag
    28981 atatcccgtg gcaccggctt taagcatttg cattacaaac ctgcgatcgg aatgcattga
    29041 caatgcgatt acctgcacgc ctttgaattc tgttgttatc tttgtagtag catcaatacc
    29101 attcatctca ggcattccaa tatccatgat caccacgtca ggatgaagtt tttgaaccag
    29161 gttcaccgtt gtgagaccat tatccgcctc tgcaattacc tccatgtcat cctgcttttc
    29221 aaggagggaa cggaggcctt cccttataat tttgtgatca tcagccaaaa taatcttggt
    29281 tctcatttgt tatgttccct gccttttgct tttctataac aagaggggca gtaagtatca
    29341 ccctggtacc acggcctctg ccagaacgaa cttccatttg cccgctgagg tggcgaagcc
    29401 tttctctgat actaaaaagc ccgaatccct tattttcgtc tcggtaaaaa ctcatttttg
    29461 atggaacaaa tccgattcca ttatcagcta cgtgcactgt aatgctgtca ttaacacggc
    29521 gtatagtgat tttcgctaca tttgcctggg catgtttggt aacattcatg agcaactccc
    29581 tcacggcact gaaaagtact atacggattt catcacttac gggctttgga taggcgtctg
    29641 cttcaaacat acagtttatt cgctggtgct ccctgacctg ctcggccagc cattcaagag
    29701 cagcctcaag gccaaggtcg tatagaatgg gcgggctcag ctcgaaggtc agggaccgcg
    29761 tgtactgtat ggcctgctct atatgattgc gcacctcttt caactggttc atggattcat
    29821 cattgcctat caactgcgca agtgccccga gcttgatctt tgaaatagca agaatttgac
    29881 caatatgatc atgaagcatc gtagcaattc ttcgcctctc acgttcttcc gccaatgata
    29941 gatcagaagc cagtgatctt agttgtttct gatatgattt aatcttttcc tctgcctttt
    30001 tttgctctgt aatgtcttca actgtgcctt catagtaaag aagtgtcccg tctggtgagc
    30061 ggactgcctt agcgttctcc cttacaaaaa tgcttgtttt atctcgcttc cgccattttg
    30121 tttcaaatcc tcttacccgt ccctctttct ccagtctgtc ttttatcttc tttctcagat
    30181 acgatggttc ataatccctt cttttccctc ccacagacac cagttcatca aatgatgaat
    30241 aacccaacat tcgtatcaat gttgggttac ccattaaaat ccgaccctca gcatttactc
    30301 gataaattcc cagcggtgaa ttgacaaaga gatttcgata tctttctgct cctttccgta
    30361 gcatgtcttc aatacgtttt cttctgttaa gctctactat aaggcggtca ttcgcatcct
    30421 gaagttctgc tgtccttgct gcgactcgtg cttcgagctc ttcttttgat ctttgtagtg
    30481 caatctgtgt ctgttttctt tcggtaatat cgttatttat ttccataatt gccactggcc
    30541 tgtttttctg gtcacgcctc aacgccagcc ggctttccac tacaatctct tctcctgacc
    30601 tctttttttg tacaagctcc ccctcccacc ttcccacgat tgctaactta gcttttatat
    30661 caataagcgg ctccggaaac accgtgtgga gaactgcata tgacacttga ttcttaacct
    30721 catcctttgt ccagccatac ctttcttccg ctcctgcatt ccaaaaaaca atcctgtctt
    30781 ccatatcgag cacaatgatt gcatcttccg caatatccag aagttctgcc tgctctttga
    30841 gttgctttgc ttgatttctc agcttctctt ctgcttttct tctctccgtt acattccgca
    30901 agttcaccat taccacctga gcttcaccat agtggattaa tgttccactt atttctacat
    30961 caatcacaga tccgtttttg tgccgataat ggcgcaggcc atagatgtat gttccgtccc
    31021 gtaagacttt cctgatgctt gcatctatca tctctttccg gctgataaca atgtcataga
    31081 gtgataaccc gggtatttca tcttcggaat atcccagcag ttttgtgaat tgatcatttg
    31141 cttccagtat tctggcggtc tcagggtcga agatatatac tccatctgac gattgtttca
    31201 c
//
LOCUS       DAOOMC010000018        30252 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdus sp. isolate AMR_MDS_2685
            NODE_1323_length_30252_cov_6.529324, whole genome shotgun sequence.
ACCESSION   DAOOMC010000018 DAOOMC010000000
VERSION     DAOOMC010000018.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864760
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdus sp. (wastewater metagenome)
  ORGANISM  Syntrophorhabdus sp.
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae; Syntrophorhabdus.
REFERENCE   1  (bases 1 to 30252)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 30252)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,729
            Genes (coding)                    :: 2,708
            CDSs (with protein)               :: 2,708
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 7 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30252
                     /organism="Syntrophorhabdus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1323_length_30252_cov_6.529324"
                     /isolate="AMR_MDS_2685"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2699756"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..364)
                     /locus_tag="PK125_03100"
     CDS             complement(<1..364)
                     /locus_tag="PK125_03100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB37126.1"
                     /translation="MFRSLWVKFLILLVGVAVLSLSGTFALRELMLRDFQAYVEGETE
                     DKIYWIIADFEGAYDRDNGWNAEVQSQNLIWAFTLGFEIRLLDETGNLVIDTEKAIEK
                     TNPQAKRRLLALSQFRELK"
     gene            complement(357..1058)
                     /locus_tag="PK125_03105"
     CDS             complement(357..1058)
                     /locus_tag="PK125_03105"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HPB37127.1"
                     /translation="MVYPILVVEDDSKIGHIVKVYLEGAGFRVIHVNRGAQALEEVRR
                     EKPLLVILDLMLPDMSGEEVCLQLKELADVPIIMLTAKSSEEERVAGFALGADDYVVK
                     PFSPRELVFRVKAVLKRFEKKDLTGEGPIRFNKGALVVDGNTYKVLKTGKEIRLTPTE
                     FKVLFTLASSPQRVFTRAELVEKALGYQFEGYERSVDAHIKNIRHKLEDDSQNPVFIH
                     TIYGVGYRFSGNRDV"
     gene            1166..1978
                     /locus_tag="PK125_03110"
     CDS             1166..1978
                     /locus_tag="PK125_03110"
                     /inference="COORDINATES: protein motif:HMM:NF012993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HPB37128.1"
                     /translation="MKAGFYQFNPVFGNKKKNITSVANALKGIDVDLLVLPEFFATGY
                     QFTSMEEVGYLAETIPDGETTEALASLSKKYGMYMVAGLAERFDKHYYNSAVLTGPEG
                     FVGLYRKTHLFFEEKLWFTPGNTGFNVFQTGIGPVGIMICFDWLFPESMRTLALQGAQ
                     VIAHPANLVLPHCPSAMPIRCLENKVYAITANRTGREQRKEGSCLEFIGKSQIVAPDS
                     TIIAKAPKKWTSLMIADLKIDTALNKSINQYNDLFKDRRPEMYQVLVEQETK"
     gene            1993..2835
                     /locus_tag="PK125_03115"
     CDS             1993..2835
                     /locus_tag="PK125_03115"
                     /inference="COORDINATES: protein motif:HMM:NF021507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HPB37129.1"
                     /translation="MWKKAWLIKTSMIVILISVLISDSTGQDSVWKQIDDGLHYCDFS
                     APAKSTAGDSKILVVRIDPKLYAFRLLSISELSPHSPSLTVKEWADKYKLIAAINAGM
                     FQTDLKSNVGYMKNYSHINNPRFHRTYSSALAFNPRKGDLPPVMVFDTDVKHMKEIVA
                     EYHTVIQNLRLVKRPRSERWAQQEKRWSEAALGQDQDGNILLIFSGSPYTMHELIKIL
                     LALPIKLDCAMHLEGGPAASFYLRYNTIELTRVGRHELSSLVGEMGNELLPIPNVIGI
                     TKKN"
     gene            complement(2854..3126)
                     /locus_tag="PK125_03120"
     CDS             complement(2854..3126)
                     /locus_tag="PK125_03120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB37130.1"
                     /translation="MANISKIPIQALWIITALTATILMLTMVQVADAPILKALTPSEG
                     YAYQIDNHGRIMDRYGRLRGWMKGAEVYSPSLELKYRLFGRQLRDF"
     gene            complement(3131..3292)
                     /locus_tag="PK125_03125"
     CDS             complement(3131..3292)
                     /locus_tag="PK125_03125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB37131.1"
                     /translation="MANEEQNKMNEEQERINGKKEGKARKASSNRNVTTEIRSKVEAE
                     YRSEKLTRR"
     gene            complement(3882..4508)
                     /locus_tag="PK125_03130"
     CDS             complement(3882..4508)
                     /locus_tag="PK125_03130"
                     /inference="COORDINATES: protein motif:HMM:NF013933.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family translocator"
                     /protein_id="HPB37132.1"
                     /translation="MIDPAILNLFIPASLLLIIAPGPDILFVVAQGISSGRRAGFFTA
                     LGLAFGNSVHTLAAALGVSLIFKTSVMAFTVFKIAGALYLFYLAYKVIKHRKELMKMS
                     KQDSKPIHYLIAKGFIMNVLNPKVALFFLAFLPQFVAKNAGNPFGQILILGAIFIILV
                     VIIFGAIGLTAGTFGQFLTRSRTANETLNWLCAFVFIGLGIKLVLSRA"
     gene            complement(4528..5136)
                     /locus_tag="PK125_03135"
     CDS             complement(4528..5136)
                     /locus_tag="PK125_03135"
                     /inference="COORDINATES: protein motif:HMM:NF013077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HPB37133.1"
                     /translation="MLTTKDAIQQRRSIRKFKSYPVPDEVIFELLDAARLAPSGSNAQ
                     PWRFKIVQDNETKERLAKAAYDQFFIAEAPVVIVCCADISGYLSGVVSGLQDLGRIGA
                     VQDRIVNIVLDRTDRMQKMSFDQFSQRIAFNVAIAIEHIALRALDFGLGTCWVRLIDE
                     EAIRAIFGWDETVSVVALLPVGYPGESPAPRKRQPIEEILID"
     gene            complement(5148..5342)
                     /locus_tag="PK125_03140"
     CDS             complement(5148..5342)
                     /locus_tag="PK125_03140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB37134.1"
                     /translation="MRQDNNDQTPFCDYLRGYGYSEAAGNYDLLAEEYNECRWLSEDD
                     QKTRLNSIIRLLELEARTLF"
     gene            complement(5903..7093)
                     /locus_tag="PK125_03145"
     CDS             complement(5903..7093)
                     /locus_tag="PK125_03145"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HPB37135.1"
                     /translation="MLSRRAQALKPSPTLAINAKEKLLRAQGLDIAGFGAGEPDFDTP
                     EHIKQAAIDAVNKGFTKYTPVAGIEPLKDAIIEKFKNDNGLTFKREEVIVSCGGKHGL
                     YNLFQALFQEGDEVIILTPYWVSYPPMVELAGAKPVIVDTREEDDYQVTGDLIRKYIT
                     SKTKGIVLNYPSNPVGSVYTVENLVQIGRLAVDHNLYIISDEMYEKIVYDGYQHTSIA
                     SIDSAFRERTIICNGVSKAYSMTGWRIGYTVGQKEIIAAMSNIQSQSTSNPTSISQYA
                     ALAALTGPQDFISMMVGEFQKRRDFLVRELTSIPGVTCYNPRGAFYVFPNFNALLGKR
                     FNERTIDSSAALTELLLEEFHTAVVPGAEFGKEGYLRLSFATSMDVIKKGAERIQKAV
                     ASLA"
     gene            complement(7094..7648)
                     /locus_tag="PK125_03150"
     CDS             complement(7094..7648)
                     /locus_tag="PK125_03150"
                     /inference="COORDINATES: protein motif:HMM:NF014886.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase alpha/beta domain-containing
                     protein"
                     /protein_id="HPB37136.1"
                     /translation="MYFEKAGIENTDVTLKTAFNHAANKGIKDIVVASTTGYTAERIL
                     QIPGREAFNIVVVTHNTGFKEEGVQSFPVDLRQRLIEHGIRIHTGTMVLRGLGTAIKD
                     MTGGYSEQELIANTLRMFSQGVKVCVEISAMAADSGVIPFDDVITIAGTGRGADTACL
                     IRANSSNRFFKIRIKEIIAKPKEA"
     gene            complement(7795..9180)
                     /gene="tilS"
                     /locus_tag="PK125_03155"
     CDS             complement(7795..9180)
                     /gene="tilS"
                     /locus_tag="PK125_03155"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HPB37137.1"
                     /translation="MDDLVKKTKDIIRREGLIAKGDKVLVACSGGIDSITLLFVLREI
                     GHELPIRLGVAHVNHLLRGLESDRDEDFVRGLADRFSIPCYVKKINVSYEARKSGKSI
                     QHAGRDIRYCFFDEIADALHFDKIAIGHTLDDQVETFLLRIVKGTGIRGLSSIPIMRG
                     RIIRPFLTTYRSTIEEYTKTRGIPFVKDSSNEKNQYERNFVRRVIIPAMEKLNPAVKN
                     KIVALLKDMTAVNIFFEHEANVFINEKKSYENDDITIDAESMQGLDEEVRYRVLFSLF
                     QELEPSFLPLREHVRLVGKILKGKGPNLLATFPKGIHVKKTYGKITFTKKGEALPVEG
                     HYRVSTGRNIIESLGLTLEVNEIDEMDEQSGPIPIAPNIAYFDLEKLGGLSVRTFKAG
                     DRFVPFGMSQEVKIKDFFIGRKIPKEKRRRVPLLLSNHDIIWVVGYRIDERYKVTKET
                     NHVMKVIAHFN"
     gene            complement(9523..10413)
                     /locus_tag="PK125_03160"
     CDS             complement(9523..10413)
                     /locus_tag="PK125_03160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB37138.1"
                     /translation="MKPSNLEKKQIDLINESLCVYTKCMHCKDLYMKGMLCFGDVEEF
                     VDDRGRSCLFRLKEMCHELFRNSDAATYREKLYDMTVGYIFHQAMILRENLYQLEYYK
                     PKCDIDTNELTIREKKIFLEMEFLVKKTERKLKQGFKETKLLLKELVEQLKDLIKLYK
                     DNYLLPRFLFEHEKTLTMIYGKRNFNQLLNDLYKDGRRLLMFNAAQSYLESEYYEIAR
                     NIFQKVTDLDASNRLAHFLFMYASAFHFFYKNHFLRAYRYVENAMGFDLNDNDKAPYL
                     TALTKLENEIKKDMTRSRRP"
     gene            complement(10400..12295)
                     /gene="ftsH"
                     /locus_tag="PK125_03165"
     CDS             complement(10400..12295)
                     /gene="ftsH"
                     /locus_tag="PK125_03165"
                     /inference="COORDINATES: protein motif:HMM:TIGR01241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HPB37139.1"
                     /translation="MPRKRKEPQKSKNIFLELKDKTKLKDKKTKNFTFLYFILAFVAM
                     IAINSYLFKSEVKNIPYSEFKELITKGKISDLIIDTDTIQGTLTLDDGKKTKFLTSRV
                     EDPDLVKDLQKNNIKFAGYYENKIFKAIISWVLPFAIIFLIWNLLMRRMGGAPSSVLN
                     FGKSRGKIYGEDEIKITFQDVAGVDEAKEELQEIIEYLRTPQKFMTLGGKIPKGILLV
                     GPPGTGKTLLAKAVAGEAKVPFFSMSGSDFVEMFVGVGASRVRDLFAQAQEKAPCIIF
                     IDELDALGKARGMNPMSSHDEREQTLNQLLSEMDGFDTKAGVIIMAATNRPEILDPAL
                     LRAGRFDRHILVDRPDIKGREEILKVHIKGVKLAEEVDLKVVAARTPGFVGADLANLV
                     NESALLAARKGKTSVGMEEFEEAIDRVIAGLEKKTRVMNKKEKEIVAFHETGHALMAE
                     FLETTDPVHKISIIPRGISALGYTLQLPTEDRYLMTEKELRDRLCVLLGGRVAEEIIF
                     GEVSTGAHNDLGRATDIARSMVKEYGMSKKLGYVTFERERKPLFIELGTSFSGVKDYS
                     EETAREIDDEIKMIVEEAREKVRTLLGGKKALLEKVATTLLEKETIEGEELRELISNH
                     SAGHETK"
     gene            complement(12510..14132)
                     /gene="serA"
                     /locus_tag="PK125_03170"
     CDS             complement(12510..14132)
                     /gene="serA"
                     /locus_tag="PK125_03170"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: protein motif:HMM:TIGR01327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004617 - phosphoglycerate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HPB37140.1"
                     /translation="MKRYKILITDNISEEGIEILNREGDIDVDIKYGIKSDELKKVIG
                     EYDALITRSGTSITAGILEKPGRLRIIGRAGVGIENINVEAASKKGIIVMNAPTGNTL
                     AATELTMGIMLAAARKIPLANNSLKEGKWDRKRFMGIQLYNKILGIVGLGRIGSAVAI
                     RAKSFGMKVIAYDPYIKKSKADSLGVILYDKLEDLLKIVDVVTFHTPLTPATRNMIKA
                     EEIAMMKHNVIFVNCARGGIVNEADLYDALKTGKVFAAGVDVWEKEPPQSTRLLELEN
                     AFATPHIGANTMEGQEAVALIICNQVANALHGRPYENAVNIPFMLSQLPENMQLYFNL
                     VEKMGKLAAQMVKGRPELIRVVMVGKTFEEDLCERKFDVPFSYQPFTIAGVKGFLAEN
                     LKETVTFINAPYFAKDRNIVVEEAKTDQFDKFNDLVLLVIRTDTEERSFAGTVFPDKL
                     GRIVLLDRFHLDMIPEGTFLHFKIFDRPGIIGKVGTILGNHKINIAGFGVSRQKSGEE
                     VAFVSVDDPISKDVLNEILRIDGMIEASVLQL"
     gene            14510..14956
                     /gene="rplM"
                     /locus_tag="PK125_03175"
     CDS             14510..14956
                     /gene="rplM"
                     /locus_tag="PK125_03175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020063210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HPB37141.1"
                     /translation="MKTYFPKTDEIVKDWYLISAEGATLGRLAAQIAMMLRGKTKPTF
                     TPHTDVGDFVVVINAEKIRLTGRKIDLKTYARHSGYHGGFKSVNMKTMLEKKPEEVIR
                     LAVRGMLPKNALGRKIIKKLKIYKGAEHPHKAQMPKEFVYKEVRGA"
     gene            14949..15341
                     /gene="rpsI"
                     /locus_tag="PK125_03180"
     CDS             14949..15341
                     /gene="rpsI"
                     /locus_tag="PK125_03180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016293468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HPB37142.1"
                     /translation="MPEKRYYATGKRKTAVARVWIKLGAGNFVINGRAIDDYFPLEEL
                     KLMVNKPFLLTGNLGKFDVIANIYGGGVPAQAWALGHGIAKALLEYNINFRTTLKKQG
                     LITRDPRAKERKKYGKKGARASFQFSKR"
     gene            15274..16365
                     /gene="argC"
                     /locus_tag="PK125_03185"
     CDS             15274..16365
                     /gene="argC"
                     /locus_tag="PK125_03185"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: protein motif:HMM:TIGR01850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HPB37143.1"
                     /translation="MRKKEKNTAKKEHVQASSFPRDKMLKAGIIGTTGYTGLALISAL
                     VSHPDVTVSLITSNTYKGKKISDIFPLLKGRFETILVPTDDDHAGKCDVYFLCLPHGT
                     SMEAAKKLYDGKALIIDLSADFRFEDVDTYEQVYIPHTAKELIPQSIFGLPEIYRHRI
                     KGATLIGNPGCYPTSAILGLYPLLKAGYLAGDIFIDSKSGVSGAGRETKLGSMFCEVS
                     EGFRPYGVGVHRHEPEIQKEVAKFSKLRVMFVPHLLPMNRGILTTIYSRLSQPLKTKD
                     LLALYRKTYDGEPFVRVMDEGVYPDTRFVRFSNYIDIGLKSFDDGRIVIISAIDNLVK
                     GASGQAIQNMNVALGLSETASLTAVPQFP"
     gene            complement(16673..16801)
                     /locus_tag="PK125_03190"
     CDS             complement(16673..16801)
                     /locus_tag="PK125_03190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB37144.1"
                     /translation="MAGSVYIVNAGPVVVNKNQKRMQHGGRPWQQQRALKMSALVI"
     gene            16962..17771
                     /locus_tag="PK125_03195"
     CDS             16962..17771
                     /locus_tag="PK125_03195"
                     /inference="COORDINATES: protein motif:HMM:TIGR01188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HPB37145.1"
                     /translation="MADKNIIHVSDLTKTFNGFTAVNHISFSVAAGEIFAFLGPNGAG
                     KSTTINMLTTLLRPTSGSISLNGYDPVTSPHRVRQSFGIVFQDPSLDEDLTAWENMDY
                     HGILYGISRNIRGKRSEELLKIVELWERRKEQVKKFSGGMKRRLEIARGLLHHPKILF
                     LDEPTLGLDPQTRSLIWNYIKGLNQSEGTTIFLTTHYMEEAERVAHRIAIIDHGKIIV
                     HGTPDELKAQTGTSSLEAAFIALTGRDIRDEEPNNLEQMRMRRKIFRRHQR"
     gene            17768..18514
                     /locus_tag="PK125_03200"
     CDS             17768..18514
                     /locus_tag="PK125_03200"
                     /inference="COORDINATES: protein motif:HMM:NF013245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPB37146.1"
                     /translation="MKVIYILWLRQLKRYFRSKARIIGSLGQPLLFFVALGFGFGPIY
                     TRAGGGDYIQFLAPGIITMSILFTATFSGIEVLWDRRFGFLKETLVAPVSRLQIMLGR
                     TLGGATVAVVQGLIVFILSVIVGFRPTTVSMIPLALLYMALIAITFTALGTAIASFIE
                     DMQGFQIIINFLIMPLFFLSGALFPLENLPKAVVVLSMINPLAYGVDGLRGTLISMVH
                     FGLATDFVVLGCIAVFVLLGGAYLFSRIEV"
     gene            18663..19994
                     /gene="ffh"
                     /locus_tag="PK125_03205"
     CDS             18663..19994
                     /gene="ffh"
                     /locus_tag="PK125_03205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018286751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HPB37147.1"
                     /translation="MFEKLQERLEATFKKIRGYGKVTEDNIKDSLREVRIALLEADVN
                     YKVAKDFLEKIKEKAIGEGVLTSITPGQLFVKTVHDELCELLGQVNKPLDVSGSPPVS
                     LMLIGLQGSGKTTTAGKLALQLRKKGRKPLLVPSDVYRPAAIDQLLKIGQQINIATFA
                     PKDIRDPIVICKEARIHAMKNGFDTMIIDTAGRLHINDEMVEELVKQKGLLNPRETLL
                     VLDAMTGQDAVTSATTFNEKVGVDGIILTKLDGDTRGGAALSIKAVTGKPIKFIGVGE
                     KLDALEPFFPERMASRILGMGDVVSLVEKAQEVFDEKQAKELERKLRKDEFTLEDFRD
                     QLKQMKQLGSIESIIGMIPGLNKLKGAIDFTAAEKDIRKTEAIINSMTARERVYPNII
                     DGSRRMRIAKGSGTQVHDVNDLLKKYMETRKMIKKMTKGGMKGLQRQLLFR"
     gene            20008..20265
                     /gene="rpsP"
                     /locus_tag="PK125_03210"
     CDS             20008..20265
                     /gene="rpsP"
                     /locus_tag="PK125_03210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005606737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HPB37148.1"
                     /translation="MAVKFRLSRHGSKKRPFYRIVVASSNSPRDGRFIERVGFYDPLK
                     EPMVISLDREKVKNWYKRGAKPTRTVENLFKKEGVLSEITQ"
     gene            20283..20516
                     /locus_tag="PK125_03215"
     CDS             20283..20516
                     /locus_tag="PK125_03215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HPB37149.1"
                     /translation="MLKELIEYIAKALVDNPDQVKVSEIEGEKTSVIELNVSKDDLGK
                     VIGKQGRTARAMRTILSAASTKVKKRAVLEIIE"
     gene            20516..21013
                     /gene="rimM"
                     /locus_tag="PK125_03220"
     CDS             20516..21013
                     /gene="rimM"
                     /locus_tag="PK125_03220"
                     /inference="COORDINATES: protein motif:HMM:TIGR02273.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HPB37150.1"
                     /translation="MRFIPVGRVIAGHGLNGAVKFQYYNEDVDQFDFYTSLYADIGGR
                     QTKFNLTEVRFRKGFFYLKLEDLNSPEKISFLINQELSVREVDLPLLDNDEYYDYQLI
                     GLNVVNHKHEPVGVVTQVIHTGVNDILTVTGREEILVPMVEDLISKIDLQNALIVIDE
                     DTLRL"
     gene            21010..21744
                     /gene="trmD"
                     /locus_tag="PK125_03225"
     CDS             21010..21744
                     /gene="trmD"
                     /locus_tag="PK125_03225"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: protein motif:HMM:TIGR00088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N(1))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HPB37151.1"
                     /translation="MIFTVLTLFPGIFDSPLRESITKKAVEKGLISFNIANIREYADD
                     AHKTCDDAPYGGGPGMVMKIEPIYRAMAHLEKTMGRPRYILLSPQGRIFDEETAIRYS
                     HIKHLCLVCGRYEGLDERVLDLVDEEVSIGDYVLSGGEIPALVLIDAITRCIPGVLGN
                     EQSSVDESFSRGLLEYPQYTRPDVFMDMEVPPVLLSGNHEEIRKWRRKEALKKTIFKR
                     PDLMNRFTPTDEDRKMIREIMEEMPG"
     gene            21746..22303
                     /locus_tag="PK125_03230"
     CDS             21746..22303
                     /locus_tag="PK125_03230"
                     /inference="COORDINATES: protein motif:HMM:NF021455.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HPB37152.1"
                     /translation="MSIAIIHYPVYDKNGDIVATSVTNLEIHDIARTCMTFGLKLCYI
                     VTPLAKQRTIMEKLIHHWTNGYGSRYNPARAEALKKVVLKAGVDEMVAHCQGKPILVG
                     TSTRERPGKSIAYRELQILIEKETRPLLLLFGTGWGLSDEVVERCEKMLIPIRGKGNY
                     NHLSLRVALGIILDRLFGKRGGYDE"
     gene            22296..22643
                     /gene="rplS"
                     /locus_tag="PK125_03235"
     CDS             22296..22643
                     /gene="rplS"
                     /locus_tag="PK125_03235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005590628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HPB37153.1"
                     /translation="MNEMIDLLEKEHMRLDLIETSVGDNVRVYTKIFEGDKERIQVFE
                     GVVIRRRGGNTRATFTVRKVSYGIGIEKTFPMHSPLIDKIEVLSKSKVRRSRLFYLRN
                     LRGKAAKLKEKRD"
     gene            22643..23227
                     /locus_tag="PK125_03240"
     CDS             22643..23227
                     /locus_tag="PK125_03240"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: protein motif:HMM:NF000595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HPB37154.1"
                     /translation="MVCTLTGLIGGIDEAGRGPLAGPVVSSCVVWRSLPDHPKKINDS
                     KLLSPKQRSDLFPWIQQHAFKIGIGIATHEEIDKINILRASLLSMERALHNSGVEPNL
                     VLIDGRFALQSYPHSRPVVKGDRKCFYIACASIVAKVTRDTIMNRYDLEYPQYNFRKN
                     KGYPTKDHRLAIKQHGVSPIHRKTFKGVREFLGV"
     gene            23217..23570
                     /locus_tag="PK125_03245"
     CDS             23217..23570
                     /locus_tag="PK125_03245"
                     /inference="COORDINATES: protein motif:HMM:TIGR00252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HPB37155.1"
                     /translation="MASKQEEGREGEKRAENTLKRAGYKIIEKNFRSRFGEIDIVAEE
                     NGCLVFVEVKKRNTPNFGDPFNAINARKRQHLIKCAMFYLKSHNSFNRKTRFDVVGIE
                     GEQVKIIKNAFIVEE"
     gene            23699..24442
                     /gene="larB"
                     /locus_tag="PK125_03250"
     CDS             23699..24442
                     /gene="larB"
                     /locus_tag="PK125_03250"
                     /inference="COORDINATES: protein motif:HMM:NF033503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarB"
                     /protein_id="HPB37156.1"
                     /translation="MTHKKILKLLESVKNGKTTVEQAYERLKDIPFEDLSHTKIDHHR
                     VVRKGLQEVIFGEGKSIHQINDIIRAMQNKGLDILVTRVSKETGSELTALFPVGVYNS
                     DARCFYIRKDLSVKGKGSILVMSAGTSDISIAEETYITSIFFGNKTERLYDVGVAGIH
                     LLIENMDLLKQARVIVVVAGMEGALPSIVAGMVGVPVIGVPTSIGYGASFGGLSALLA
                     MLNSCSTVSVFNIDNGFGAAYFATLINRL"
     gene            24439..25599
                     /gene="larC"
                     /locus_tag="PK125_03255"
     CDS             24439..25599
                     /gene="larC"
                     /locus_tag="PK125_03255"
                     /inference="COORDINATES: protein motif:HMM:TIGR00299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarC"
                     /protein_id="HPB37157.1"
                     /translation="MNILFIDPIFGISGDMLISAFLDAGMPFQELYDLLAKIPIDMPE
                     IKPVKMNQGIIEGIHLEIAESKTHLSITQMEQLIDDLNVDSEIKDGAKGILNVLVAAE
                     AKVHGVPRDQVHFHELSHIDTLIDVISVAKCIHYFDIEKVYCGPLPMGRGTIKISHGY
                     VPNPPPATVEILTGYSLRFIDTSLELTTPTGAAVVRYYVKDRHGIPSFTMEKIGYGLG
                     SYKSEIPDALRIFIGRSEHPSGVEEVWLLEADLDDMNMEYLGAVAERIREKGALDVLY
                     FPVYMKKGRIGIRLSVTVPAKALQSAIDSVFMETTTFGVRMQKLQRQVLKRDEKIIHT
                     PYGSVRVKNGYNRDGHLVKTHIEFNDVKKIADDKGLAYQSVLEIVKRKLDSI"
     gene            25645..27123
                     /locus_tag="PK125_03260"
     CDS             25645..27123
                     /locus_tag="PK125_03260"
                     /inference="COORDINATES: protein motif:HMM:TIGR00573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HPB37158.1"
                     /translation="MPLDDLDLAIVDVETTGTASHYHRIIEIAVLRIQSGHLVDKYST
                     LLDPERAIPPFIETLTGIKNKDLKNAPTFRQVKNHIYNLLDGAIFVAHNAKFDYTFLR
                     EEFEREKINYTAKRLCTMRLSKTLFPGLKKHNLDSIIERFGIKCSDRHRAESDASALW
                     DFLQRVKEQVDTENFRRILGKILKTPTLPPRLNDSIIKSLPETYGVYIFYDENSNPLY
                     VNKSVNIRNRVLSHLSEDQESAREQALSRRVVDIQAIATSGELGALLLESHLMQQLSP
                     LFNRKARVHKKLVALKRDLNEQKYGTIRLEHLNNLRPADLPNIVGIFKSTQRAKEFLW
                     NIAKGYTLCPRILGIEKGKGECTYSQLQRCGGVCSGHESANTYNTRFNLAFAGRRMVP
                     WPFRGPVLIEEKNVSGNGGEYFVVDNWCLLQYFRYDETGTKQCLLKGNGSFDYDGYKI
                     LRDYLLNSRKQTNVRPLTISEFTSILENEGQFKVVPRKIKQS"
     gene            complement(27356..28624)
                     /locus_tag="PK125_03265"
     CDS             complement(27356..28624)
                     /locus_tag="PK125_03265"
                     /inference="COORDINATES: protein motif:HMM:NF024825.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HPB37159.1"
                     /translation="MTKQKSLVTLVLILVGILLACGTSFAAEKAAKTKPIKIGVLFIA
                     SGPMGGYGKHGFQSVQMAVDEINASGGILGRKVEAIYEDTKLKADVATEIVKRFIEKD
                     KVDFIIGPTSSGVAVPVAKLANEKKKIIVLTQAAANSMTDTGFGRYQFATLSNAMMHS
                     RSGAYYMASKPYKRWMVIGPNYNYGHESWAAFKEKLKELRPDVEFVGEAWPKLTEKEF
                     KPFIEKIVEAKPDAVWSPLWGMDAVNFIKQAKEKQLFDKIAFAFPDGAALETLIPLGK
                     DMPNGIFVAARYFFLTPDTAINKKFVKMYQERFKELPDYMATETYAGVYFIKAAIERA
                     GTLNADKIVTAVEKEPLAWETPEGWKIMRKEDHAVVEDVVWGETDYSDKFGFAILKNM
                     QAIQAEEICRTPEELRAVRENFMKRIRPDN"
     gene            28937..29197
                     /locus_tag="PK125_03270"
     CDS             28937..29197
                     /locus_tag="PK125_03270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB37160.1"
                     /translation="MYKEEFRTAKDGYRNKNHVCAEGYRESCESCVTEPIDVLALCKA
                     PCKTRLDEAAANCRGAFAKGTPERDMCIDHAQVVTFQSSDEC"
     gene            29203..29466
                     /locus_tag="PK125_03275"
     CDS             29203..29466
                     /locus_tag="PK125_03275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB37161.1"
                     /translation="MATILESMQLYLKDMNDRMQATTAISAVTIAQAPLKHRRSQGRE
                     YRHRINVCSVFFVIRWAQKNISKRSFAALQIGQVSGACSRAQR"
     gene            complement(29534..29875)
                     /locus_tag="PK125_03280"
     CDS             complement(29534..29875)
                     /locus_tag="PK125_03280"
                     /inference="COORDINATES: protein motif:HMM:NF014939.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016730 - oxidoreductase activity, acting
                     on iron-sulfur proteins as donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-thioredoxin reductase catalytic
                     domain-containing protein"
                     /protein_id="HPB37162.1"
                     /translation="MPDEVQELFDKLKKDAQASGYNLNTDEAFVTELIKGLIVNQKRY
                     GYNSCPCRLSEGDKWKDMDIICPCDYRDLDVSEFGSCYCALYVSEAVARGEKKFEPVP
                     ERRFLSQEKKH"
     gene            complement(29893..30177)
                     /locus_tag="PK125_03285"
     CDS             complement(29893..30177)
                     /locus_tag="PK125_03285"
                     /inference="COORDINATES: protein motif:HMM:NF012675.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin family protein"
                     /protein_id="HPB37163.1"
                     /translation="MAKQRVDGEDRGSIQLYALSTCVWCKMTKALLEKMNIGFDYVYV
                     DQLQGEEKEQALEEVKRWNPKCSFPSLVVHGKKCVIGYKEDEIKKAIDTV"
BASE COUNT         8565 a         6803 c         6364 g         8520 t
ORIGIN      
        1 cttttaattc tctgaattgt gaaagcgcca gaagcctcct ctttgcctgg gggttcgtct
       61 tttcgattgc tttttctgta tcaatgacca agtttcctgt ctcatcaagc agccgtatct
      121 cgaaacccag agtgaatgcc catatcaggt tttgtgactg aacctctgcg ttccacccat
      181 tgtctctatc gtacgcccct tcaaagtcag caataatcca ataaatctta tcttctgtct
      241 ctccttcaac ataagcctga aaatccctga gcatgagttc cctcagggca aaggtacctg
      301 acagggacag gactgcaacc ccgacaagga ggataagaaa cttaacccag agacttctaa
      361 acatctctgt tgcccgagaa ccggtaccct actccataaa tcgtatgaat aaatactggg
      421 ttttgtgaat catcttccag cttgtgacgg atattcttaa tatgggcgtc aacacttctc
      481 tcataaccct caaactggta gcccaatgcc ttttcaacaa gctcggccct tgtaaaaacc
      541 cgttgaggcg aactagcaag tgtgaacaaa accttaaact ctgtgggtgt taaccgtatc
      601 tctttgcctg ttttcaacac cttgtaagta ttgccatcca caacaagagc acctttattg
      661 aaccttattg gaccttcacc ggtcaggtcc ttcttttcga accgtttcaa aacagccttc
      721 acgcgaaaga ccaattctct cgggctgaaa ggctttacca cataatcatc ggcgcctaag
      781 gcaaagccgg cgacccgttc ctcttctgaa gattttgccg tcaacatgat gattggtaca
      841 tcagcaagtt ctttgagttg taggcacact tcttcaccac tcatatctgg aagcatgagg
      901 tcaaggatca caaggagcgg tttttccctt ctgacttcct caagggcttg tgccccccga
      961 tttacatgga tcaccctgaa cccagcaccc tccaaataca ccttgacaat atggccaatc
     1021 ttggaatcgt cttcaacgac tagaattggg tagaccatac atcattttac agcagaagat
     1081 tagaattcac aagtactcag aaataaacaa cacttaactg ctcaaccttg tctatcgagt
     1141 gtggtcatgc tattatagaa atactatgaa agcagggttt tatcagttca atccagtttt
     1201 tggcaacaag aagaaaaaca ttacaagcgt ggcaaatgct ttgaaaggaa tcgatgttga
     1261 tctcctggtt ctacctgagt tttttgccac aggttaccag tttacctcca tggaagaagt
     1321 aggataccta gctgaaacaa tacccgatgg agagacaact gaagcccttg caagcctctc
     1381 aaaaaaatat ggaatgtata tggtggctgg actcgccgaa agatttgata aacattatta
     1441 taattctgca gtcctgacag gaccggaagg ttttgtcggc ctctaccgta aaacccatct
     1501 cttcttcgaa gaaaaacttt ggtttacacc aggcaataca ggattcaatg tttttcaaac
     1561 aggcataggc ccagttggca tcatgatctg ctttgactgg ttattcccgg aatccatgag
     1621 aaccctcgcc ttacagggcg cccaggtcat tgcccatcct gcgaatcttg ttttgcccca
     1681 ttgcccttct gctatgccca taagatgtct tgaaaataag gtctatgcta taacagcaaa
     1741 cagaacaggt agggagcaaa ggaaggaggg atcttgcctt gaattcattg gtaaaagcca
     1801 gattgtcgca ccggattcca ccataattgc gaaggcacct aagaaatgga catccctgat
     1861 gattgctgat ttaaagattg atactgcact gaacaagtcg attaatcaat acaacgatct
     1921 ttttaaagat aggaggccgg aaatgtatca ggttcttgtg gaacaagaaa caaagtgacc
     1981 cttagcaaaa acatgtggaa gaaagcctgg ctgatcaaaa caagcatgat tgtgatcttg
     2041 atttctgttc ttatttcgga tagtactgga caggattctg tttggaagca gattgatgat
     2101 ggccttcact attgcgattt ttcagctcct gcgaaatcaa ctgctgggga ttcgaaaata
     2161 cttgttgtca gaatcgaccc gaagctttat gcttttcgcc tcctttccat ttctgagttg
     2221 agccctcact ccccttcact gaccgttaag gagtgggcag acaaatacaa gcttattgcc
     2281 gcaatcaatg ctggaatgtt tcagacagac ctgaaatcaa acgtaggcta tatgaaaaat
     2341 tattctcata taaacaatcc gaggtttcac agaacctatt cctctgcact ggcctttaat
     2401 ccacgaaaag gggacttgcc gcctgtaatg gtattcgata cagatgtaaa acatatgaag
     2461 gagattgtcg ctgaatacca taccgtgatt caaaatctta gactggtcaa gcggcctcgt
     2521 tcagaacgat gggcccagca ggagaaaaga tggagcgagg ctgctctggg acaggatcag
     2581 gatggaaaca ttctgctgat cttttcaggg tcaccataca caatgcatga actgattaaa
     2641 attctgttag ccctgcccat taaactggac tgtgccatgc atcttgaagg tgggccggca
     2701 gcatcctttt acctccggta taacactatc gaactcacaa gggtgggccg ccatgaatta
     2761 tcatcccttg ttggcgaaat gggcaatgaa ttgcttccta ttcctaacgt tataggaatt
     2821 acaaaaaaga actgaccgcc agaccggctc taatcagaaa tctcttaact gtctaccgaa
     2881 taatcgatat ttcagctcaa ggctggggct gtatacctca gcgcctttca tccatcctcg
     2941 cagccgccca tatctatcca tgatacgccc gtggttatca atctgatacg cataaccctc
     3001 actcggcgta agcgctttca aaatcggtgc atcagccacc tgcaccatcg tgagcattaa
     3061 aattgttgct gtaagggctg ttattatcca tagtgcttga attggtattt tggaaatatt
     3121 tgccataagt tcaccttctc gtgagttttt ctgaccggta ctcagcttct acttttgacc
     3181 ttatctccgt tgtaacgttt cgattcgaac tcgcctttct cgccttacct tccttcttcc
     3241 cattaattct ttcttgttct tcgttcatct tgttttgctc ttcatttgcc attatttcat
     3301 tttacagggt ggacatgaca actgtatgtc aggacatact gataaaaatt ttttggcact
     3361 ttttttatgg cctctatccc gccaattaac tttatatgaa tgacagcctt tttctgacat
     3421 gtggtgacaa aaacagtatt agggaacaga cggataagaa ctgccggacc cattccttgg
     3481 cacaaagccg aggcaaaaag tgacagcccg gtagtttatc cagttattag gttgactcag
     3541 gtgaccagga tcaacaatac tgtattgatt accagccaga tatttttttc tgaatggcct
     3601 cgaagcaatc gctgaatcaa tcagtccaca aagcagcagt atctattcct gggtgtagta
     3661 atacaaaatc cttttttcaa ggtcgctgcc tccaaacaga agaaataaca gagtaacatg
     3721 aaatttcaag agttcaacag atcaagaccg tgtatccggt tccagtgtgc taatcgtgta
     3781 attatccatt caacccctac aatcacacca cgcagctaat agacagaaaa tcagtggacc
     3841 aacataatgg tctggcaatt ctccttgttc taagcccgtg tttacgcccg gctcagtacc
     3901 agcttgatcc cgaggccaat aaacacgaaa gcacagagcc agtttagagt ttcgtttgca
     3961 gtgcggcttc tggtgagaaa ctgaccgaat gtccctgctg tcaggcctat agcaccaaaa
     4021 atgataacga cgagaattat aaaaatggct ccaaggatga gaatttgtcc aaagggattt
     4081 cctgcgtttt tagcaacaaa ctgtggcaaa aaggcaagga aaaagagagc caccttcggg
     4141 ttcagaacgt tcatgataaa accttttgct ataaggtagt ggatgggctt gctgtcctgc
     4201 ttactcattt tcatgagttc tttacggtgc ttgattactt tgtaggcaag gtaaaaaaga
     4261 tacagtgcgc ctgctatctt gaagactgta aaggccatca cagaggtctt gaagattagc
     4321 gacacgccaa gcgccgctgc cagcgtgtgg acagagttcc cgaatgccag accgagggcg
     4381 gtgaaaaaac ctgctctcct acccgatgaa atcccctgtg ccaccacaaa gaggatgtca
     4441 ggccccggcg cgattataag cagaagcgat gcagggataa aaaggttcaa tatagcagga
     4501 tcaatcattt attcttccga cactttttca atcgattaat atctcctcaa ttggttgtcg
     4561 ttttcttgga gcaggagact cgccagggta tccgacgggg agtaacgcaa caacggagac
     4621 tgtctcatcc catccgaaga ttgcccgtat cgcctcttca tcaatcaacc tcacccagca
     4681 tgtcccaagg ccaaaatcca gagccctcag ggcaatatgc tcgatggcaa tagccacatt
     4741 aaaagcaatt ctctggctga actggtcgaa gctcattttt tgcatccgat cggtcctatc
     4801 gaggacaata ttgacaatcc tgtcttgcac agcgcctatt ctgccaaggt cctgaagccc
     4861 tgacacgacc ccgctcaggt atccgcttat atcagcacag cacacaatta cgacgggggc
     4921 ttcggcaata aaaaactgat cgtatgcggc cttggccaat ctctcttttg tctcattatc
     4981 ttgtacaatc ttaaatcgcc acggctgggc atttgagcct gatggggcaa gtcttgcagc
     5041 atcgagcaac tcaaagataa cctcatccgg tacagggtat gacttgaatt ttctgatact
     5101 tcgtctctgc tgaatggcgt cttttgtggt taacatatga ttcaatttta aaacagtgtc
     5161 cgcgcctcaa gctccaggag cctaataatg ctgtttagtc ttgttttctg atcatcttca
     5221 gacagccacc ggcattcatt gtattcttct gcaagaagat cgtagttacc tgccgcctca
     5281 gagtaaccgt accctctcag gtaatcgcaa aaaggtgtct ggtcattgtt atcctgtctc
     5341 atggcgttcc aaaaaagata cggctaaggc tcgattcaat cacggatgca ttgctctgag
     5401 aaatagattt ttgcaataag aatatatggc caactgcaat tctatttatc tgtaacagat
     5461 tcgtttcatg caggaacaac ataaagtatg tggggcaata gataaatgcc caacctgtgc
     5521 ttgaattcgc tgatatttca tgggattgat cggttatacc ttgttcggaa aatattagga
     5581 atatgataaa aataagcaat aggcatggga atgttttttt agtattcatc atattagccc
     5641 cccccttccg agttgtaagt atttcaaccc attccagccg ggggttcagt aaaacttaca
     5701 ggaaatcaaa tcatctaaag ctgccaacta ttttagatcc ttacatcaat tataccattt
     5761 cgagaattga aaaaagagga ttgtggataa atcggaataa tcatcggagc aggatgaaaa
     5821 cctgccccga ttatagcttt tttaaaatca gtgataaaat atgggtaaga aaaataccct
     5881 cttgccgagc ccttctcttg cctcatgcaa gcgatgccac tgccttctga atcctttccg
     5941 cacccttctt gataacatcc atggaggtgg cgaatgatag tctcagatag ccttctttgc
     6001 caaactcagc tccggggaca actgcggtat ggaattcctc taacaagagt tcggtgagtg
     6061 cagcagaaga gtctattgtc ctttcattga atcttttacc gagcaacgca ttaaagttgg
     6121 ggaaaacata aaatgcaccc ctggggttgt aacaggtaac cccaggtatg cttgtgagtt
     6181 cgcgaaccag aaaatccctt ctcttctgga attcgcccac catcatggaa atgaaatcct
     6241 gggggcctgt cagggcagcc aatgcagcgt actgtgaaat agaggtcggg tttgatgtac
     6301 tctggctctg aatgtttgac atagcagcta ttatctcttt ttgccctacg gtatacccta
     6361 ttctccaacc agtcatagaa taagccttcg aaacgccgtt acagatgata gtacgctccc
     6421 tgaaggcact gtcaatggaa gcaatgctgg tatgctggta accatcatag acaatcttct
     6481 cgtacatctc atcagagatt atataaaggt tgtggtccac tgcaagtctt ccaatctgga
     6541 caaggttttc cactgtataa acagacccga ctggattaga gggataattc aggacaatgc
     6601 cttttgtttt gctggttata tatttcctga tgagatcacc agtgacctgg tagtcgtcct
     6661 cttcccttgt gtcaacgatg acaggctttg ctccggcaag ctccaccatg ggaggataag
     6721 aaacccagta tggcgtaagg attattacct catctccctc ctggaaaagt gcctggaaaa
     6781 gattgtagag gccatgcttt cctccacacg acacaatgac ctcttcccgc ttgaatgtga
     6841 gtccattatc attcttgaat ttttcgatga ttgcatcctt caggggctca ataccagcaa
     6901 caggtgtgta tttggtgaaa cccttgttga ccgcatctat tgctgcttgc ttgatgtgtt
     6961 ctggtgtatc aaaatccggt tcacctgccc cgaaccctgc tatgtctaat ccctgtgccc
     7021 tcagcaactt ctcttttgca ttgatggcca gagttgggga tggtttaagc gcctgtgccc
     7081 ttcgcgatag catttaagcc tcctttggtt ttgcaattat ttcttttatt cttatcttga
     7141 aaaatctatt cgatgaatta gcccgtatga ggcacgcagt atctgcacca cgcccagtgc
     7201 ctgcgatggt aataacatca tcaaaaggaa tcacaccgct atctgctgcc attgcagaaa
     7261 tctccacaca aaccttaaca ccctgggaga acatacgaag ggtattcgca ataagctctt
     7321 gttcagaata tccgcctgtc atatctttga tggcagtccc caggcccctc agtaccatag
     7381 taccagtgtg gatcctgatg ccatgctcta tcagtctctg tctgaggtca actggaaaac
     7441 tctggacacc ttcttccttg aaaccagtgt tgtgggtcac cactacaatg ttaaaagcct
     7501 ctctaccggg aatctgaaga atcctttcgg ctgtataacc tgttgtggat gctacaacga
     7561 tgtctttgat tcctttgttt gctgcatgat taaaagcagt cttcaatgtc acatcagtat
     7621 tctcaatacc agctttttca aagtacataa tgaaaccccc attcattgat gatgattcac
     7681 ggttcgcagt aaatagcaac aatgagtctc ttaccgtgaa ctatttctca aaagcattaa
     7741 cgatgtacta ttgggcttgt gaaaatagtg tttaccttat ctttctgttt attgtcaatt
     7801 aaaatgagca attaccttca taacgtgatt ggtttctttt gtgactttat atctttcatc
     7861 tatgcgatat cctactaccc aaatgatatc atggttggag agaaggagtg gcacacgtct
     7921 tcttttttct tttggaatct ttctccctat aaagaagtct ttaattttta cctcttgact
     7981 catgccaaaa ggcacaaatc tgtcccctgc cttaaaagtt cttacggata agccaccgag
     8041 cttttcaagg tcgaaataag cgatgttcgg ggctatgggt atggggcctg actgttcatc
     8101 catctcatct atctcattca cctcgagggt gagccccagt gattctatga tgtttctccc
     8161 ggtggagact ctgtaatgcc cttccactgg caaagcctcc cccttctttg taaaggtaat
     8221 ctttccgtat gtcttcttta catggattcc ttttggaaat gttgccaaaa gattaggccc
     8281 tttccccttt agaatcttgc caacaaggcg gacatgttcc cgaaggggga gaaaagaggg
     8341 ttcaagctct tgaaacaggc taaaaagcac cctgtatctc acctcctcgt caagcccttg
     8401 catgctttcc gcatcgatgg ttatgtcatc attttcataa ctttttttct cgttaataaa
     8461 cacattagcc tcatgctcga aaaagatatt caccgcagtc atgtctttaa gcagagcaac
     8521 gatcttattc ttcacagcag ggtttagctt ttccattgcc ggaataatca ctcttctgac
     8581 aaaatttctc tcgtactggt ttttttcgtt tgaagaatct tttacaaatg gtatacctct
     8641 cgttttggtg tattcctcga tggttgagcg ataggtggtg agaaagggcc tgataattct
     8701 tccacgcatt attggaattg atgagaggcc ccgaatgccg gttcctttca cgatccgtag
     8761 aagaaacgtt tcaacctggt catcgagggt atggcctatg gctattttgt caaaatggag
     8821 tgcatctgct atttcatcaa aaaaacagta tcgaatgtcc ctcccagcgt gctgtatgga
     8881 cttaccagac ttcctggctt cataagaaac gttaatcttt ttaacatagc aaggtattga
     8941 gaacctgtca gccagccctc tcacaaagtc ttcatcccta tcagactcta aaccccttaa
     9001 caggtggttt acgtgggcaa cgcccaacct gatgggcagt tcatggccaa tttcacgaag
     9061 gacaaaaagg agtgtaatcg agtcaattcc tccggagcaa gccacaagga ctttatctcc
     9121 cttggcaata agtccttctc gcctaataat atcttttgtt ttttttacga ggtcgtccac
     9181 ttcgtgggtt ctcggctcca agtcaggagt tatgggtttg gtttcttggt tgcaatataa
     9241 aacctgtgac caagaaaacg gagtattttt ttcagtgcgg gatcaatgac acgatgaaca
     9301 atctgtggaa ttacaggttg cgcagctcgt gcctgatgaa cctgttgaag atgatttgaa
     9361 tttgaacccc actgagaatc caaaagaaga catctttttc gtgggcttcg tattgccgca
     9421 taaaggacaa gtcggttcat catctttgaa aacaagcatt tcaaattcat ttccacattt
     9481 ttcacattta aattcataaa ttggcatttc ttgctatcct cctcatggcc gtcgagatcg
     9541 cgtcatatcc tttttaattt cattctcaag ctttgttaaa gctgttaaat atggggcctt
     9601 gtcgttatcg ttcaaatcaa aacccattgc attttcaaca taccgatagg cccttaaaaa
     9661 atgattcttg taaaaaaaat ggaatgctga agcatacata aacaaaaagt gggccaatct
     9721 gtttgaggca tcaagatctg tcactttctg aaatatgttc cttgcgattt catagtattc
     9781 actttcaaga taactttgag ctgcattgaa catcaacaac cttctaccat ccttgtaaag
     9841 atcgttaagc agttggttaa agtttctttt tccatatatc attgtgagtg ttttttcatg
     9901 ttcaaacaaa aaacggggca gtaaataatt gtctttgtat agctttatta agtccttaag
     9961 ctgttccaca agctccttga ggagaagctt cgtttctttg aaaccttgct tcaactttct
    10021 ttctgttttc ttaaccaaaa actccatttc gagaaaaatc tttttttccc gaatggtaag
    10081 ctcatttgta tcgatatcac actttggctt atagtactca agttggtaga ggttttctct
    10141 taaaatcatt gcctgatgaa agatataccc cactgtcata tcataaagct tttctctgta
    10201 cgtggctgca tcagagtttc tgaaaagttc atggcacatc tccttcagac gaaaaaggca
    10261 acttctacca cggtcgtcaa caaattcctc gacgtctcca aaacaaagca ttcctttcat
    10321 ataaaggtct ttgcaatgca tgcacttggt ataaacacac agggattcgt taataagatc
    10381 tatttgtttc ttttctagat tacttggttt catggcctgc cgaatgattg ctaatcagtt
    10441 ctcgcaactc ttcaccttca atagtctctt tttcaagaag cgttgtggcc accttttcaa
    10501 ggagagcttt cttgccaccc aacaaagtcc gaaccttttc tcgggcctct tcaacgatca
    10561 tcttaatttc atcatcaatt tcgcgtgccg tttcttcgct ataatctttg acaccactga
    10621 atgatgtgcc tagttcaata aaaagcggtt ttctctcacg ctcaaaggta acgtatccca
    10681 gttttttgct cataccatat tctttcacca ttgatcttgc aatgtcagtt gcacggccaa
    10741 gatcattatg ggcccctgtt gagacctctc caaagataat ctcctctgcc accctccctc
    10801 cgaggagaac acaaagccgg tcacgaagtt ctttttcagt cataagatac cggtcttctg
    10861 tgggaagttg taatgtataa ccgagggcag atatgccacg ggggataatg gaaatcttgt
    10921 ggacaggatc cgtggtctcg agaaattctg ccataagggc atgtccggtt tcgtggaatg
    10981 ccacaatctc tttttctttt ttgttcataa cccgtgtttt tttctcaagc cctgcaatca
    11041 ccctgtctat ggcctcttcg aactcttcca tacccactga cgtcttgccc tttctcgctg
    11101 caagaagggc tgattcattc acaagatttg cgagatcggc ccctacaaat ccaggtgtac
    11161 gagccgcaac aactttgagg tcaacctctt cagcaagctt tacgcccttg atgtgcactt
    11221 tcaggatctc ttctctccct tttatgtccg gtcggtcaac aaggatatgc ctgtcaaatc
    11281 ttccggcccg caaaagggct gggtccagaa tctccggcct gtttgtggca gccataataa
    11341 tcacaccggc ctttgtgtca aatccatcca tttcagagag cagttgattt aacgtctgtt
    11401 ccctctcatc atgggaagac atgggattca tgcccctcgc ttttcccaat gcatcaagct
    11461 catcgataaa aatgatgcag ggcgcctttt cctgtgcctg agcaaaaagg tctcgaaccc
    11521 tggatgcgcc aaccccaaca aacatctcaa caaaatcgga accactcata ctaaaaaagg
    11581 gtacctttgc ctctcctgcc acagccttgg caagaagcgt cttccctgtt ccaggaggac
    11641 caacgaggag tatacccttg ggaatcttcc cccccagtgt cataaatttc tgtggtgttc
    11701 tcaggtactc aataatctcc tgcaattctt cctttgcctc atcgacacct gccacatcct
    11761 gaaaggtgat ctttatttca tcttcaccat aaatcttgcc acggctttta ccgaaattga
    11821 gtacgctcga cggtgcaccg cccattctcc gcatgagaag gttccagatg agaaagatga
    11881 tcgcaaacgg aagcacccat gagataatag ctttaaaaat tttgttttcg taataaccag
    11941 caaattttat gttgtttttt tgcaaatcct ttacaagatc agggtcctct actctgctgg
    12001 ttaggaactt tgttttcttc ccatcatcaa gggtaagagt cccctgtata gtatctgtgt
    12061 ctattatcag gtcgcttatt ttcccttttg tgatgagttc tttaaattca ctataaggga
    12121 tattcttaac ctctgacttg aaaaggtagc tattgattgc aatcatagca acaaaggcaa
    12181 gaataaaata aaggaaggta aagttcttgg ttttcttatc tttgagtttc gtcttatctt
    12241 ttagttcaag aaaaatattt tttgacttct gaggttcttt ccttttccgt ggcatgaaag
    12301 tattttaata catatctatt ttttttgaaa gtgtggagaa gccatacaac gtacagtgaa
    12361 tttttttcat aagtgtactt cgtatgtagc cggtggtatt tcgtcagccc acaacataca
    12421 gacagaggtt tttatatctt acgatatacg taatacaaat caccctatac tgttgtccag
    12481 ggatacaggg actataatgt aaacggtttt tacagctgta agacgcttgc ctcaatcatg
    12541 ccgtcaattc tcaaaatttc attgagcaca tctttactaa tcggatcatc cacagaaaca
    12601 aaggccacct cttcacccga tttttgacgg gagactccaa accctgcgat gttgatcttg
    12661 tgatttccaa gaattgttcc taccttgcca ataataccgg gcctgtcaaa tatcttaaaa
    12721 tggagaaaag ttccttctgg aatcatgtca aggtgaaacc ggtcaaggag tactattcta
    12781 cccaacttgt caggaaaaac tgtgccggca aagcttctct cctcagtatc tgtcctgata
    12841 acaagaagta caagatcatt gaacttatcg aattgatcag tttttgcctc ttccacgaca
    12901 atatttctgt cttttgcaaa ataaggtgca ttgataaagg tgacagtctc tttgaggttt
    12961 tcggcaagga accctttaac ccctgcaata gtgaaaggct ggtagctaaa aggcacatca
    13021 aacttcctct cacacaaatc ttcttcgaag gttttcccaa ccataacaac cctgatgagt
    13081 tcaggccttc ctttaaccat ctgtgcggca agttttccca ttttctcaac aagattaaaa
    13141 tagagctgca tattctcggg aagctgagaa agcataaacg gtatgtttac agcattttca
    13201 tagggccttc catgaagcgc atttgccacc tgattgcaga taataagcgc cactgcctcc
    13261 tgtccttcca tggtgttagc cccaatatgt ggtgttgcaa atgcattttc aagttcaaga
    13321 agtctggtac tctgtggtgg ttctttttcc cagacatcaa ctcccgctgc aaaaacttta
    13381 ccggtcttta gggcatcgta aagatcggct tcatttacta ttcccccgcg cgcacagttc
    13441 acaaaaatga cattgtgctt catcatggca atttcctcgg cctttatcat gttgcgtgta
    13501 gccggtgtaa gaggcgtatg aaaagtaacg acgtcaacta tcttcaggag atcttcaagc
    13561 ttgtcatata ggataacgcc aagagaatct gccttgctct ttttaatata tggatcatag
    13621 gcaatcacct tcataccaaa actcttggca cgaatagcta ctgcgcttcc tatacggcca
    13681 agccctacaa taccaagaat cttgttataa agctggatcc ccatgaacct ttttctgtcc
    13741 cacttgcctt ccttgagtga gttattcgca agaggaattt ttctcgccgc agccaacatg
    13801 atgcccattg taagctctgt tgccgccagt gtattgccgg ttggcgcgtt cattacaatg
    13861 attcctttct tgcttgccgc ttcaacattg atattttcaa taccaacacc ggcacgacct
    13921 ataattctca atctcccggg tttttcgagg atgcctgcag taatgctagt tccacttctt
    13981 gtaatcaatg catcgtattc accaataacc tttttcagct catcactttt aataccatac
    14041 ttaatgtcaa catctatgtc cccttctctg ttgagtattt caattccttc ttcagaaatg
    14101 ttatcagtaa taagtatttt gtatcttttc atgtcgctgg cacctttcac cctttttata
    14161 tcttgatctc tgggaactgc gtttcccatg attgcttgtg aataatatca cgggtagtgt
    14221 aaaaaaagca ataaaatcgg gcaatacata atgaacgacg gattaccgga agatctggct
    14281 gctaccgttc ctgcctgtgg ataagcaagc cagaaagtca caataagtaa gcaaccaaaa
    14341 aagcttctat agctaaccca gtacttttca attgcggtta actgatatca tacaatattt
    14401 atccttgtaa cccattaccc acctcctgtt gccggtcccg ttataaactt ttccttgact
    14461 tgaaccctct aaatatatta agttaaacaa attttttaag gacaatgcga tgaaaaccta
    14521 ctttccaaaa acagatgaaa tagtaaaaga ttggtacctc attagcgctg agggggcaac
    14581 ccttggcagg ctcgcagctc aaatagcaat gatgttgaga ggtaaaacca aaccaacatt
    14641 taccccccat acagatgttg gggattttgt ggttgttatc aacgctgaaa aaatcagact
    14701 tacaggacga aagatagact taaagaccta tgcaaggcat tctggctacc atgggggttt
    14761 taagtctgta aatatgaaaa ccatgctgga aaagaagccg gaagaagtta ttagacttgc
    14821 agtcagaggc atgcttccaa aaaatgcact cggtaggaaa ataatcaaga aactgaagat
    14881 atataagggt gcagaacatc ctcataaagc tcagatgcca aaagaatttg tttataagga
    14941 ggtaagaggt gcctgaaaag cggtactatg caactggaaa gagaaagaca gccgtagcaa
    15001 gggtctggat taaattaggc gccggcaact tcgtaattaa cggtagggcc attgatgact
    15061 atttccccct tgaagaactg aagcttatgg ttaataaacc cttcctgctt accggcaacc
    15121 ttggaaaatt tgatgtgatt gccaatattt acggtggagg ggttcctgcg caagcatggg
    15181 ctttgggcca cggaatcgca aaggctctcc ttgaatacaa tataaatttt agaacaaccc
    15241 taaagaagca gggtctcatt acaagagatc cacgtgcgaa agaaagaaaa aaatacggca
    15301 aaaaaggagc acgtgcaagc ttccagtttt ccaagagata agatgctgaa ggcaggtatt
    15361 atagggacca ccggttacac cggcctggcc cttatttcag ctctcgtttc tcatccggat
    15421 gtaacagtca gtctcataac atccaacacg tataagggta aaaaaatatc tgacatcttt
    15481 cctctactta aggggagatt tgaaacaatt cttgtgccta cagatgacga ccatgctggc
    15541 aaatgcgatg tatactttct ctgtctgccc catggcacgt ctatggaggc agcaaaaaag
    15601 ctttatgatg gtaaagcgct tatcatcgat cttagtgctg attttcgctt cgaagatgtg
    15661 gatacctatg aacaagtata tatcccccat actgccaaag aactcattcc tcaatcaatt
    15721 tttggccttc cagagattta tcgtcacaga atcaaaggtg caacactcat cggcaaccca
    15781 gggtgttatc ctacctctgc aattttaggg ttatacccgc ttcttaaggc tggctatcta
    15841 gctggggata tatttatcga ttcaaagtcc ggtgtcagcg gagcagggcg agaaactaaa
    15901 ctgggaagca tgttctgtga agtctcggag ggtttcaggc catacggggt cggggttcat
    15961 cgtcatgaac ctgaaatcca gaaagaggtt gccaagtttt ccaaactaag agtcatgttc
    16021 gtaccccatc ttcttcctat gaatcggggc attctgacca cgatttacag cagactgagt
    16081 caacccctta agacaaagga tcttcttgct ctttacagaa agacatatga tggcgaacca
    16141 ttcgtgcgcg tcatggatga aggggtgtat cccgataccc ggtttgtaag gttttccaat
    16201 tatattgaca tcggtctcaa aagttttgat gatggtcgta tcgtaattat ttctgcaatc
    16261 gacaatcttg tcaaaggggc ttcaggacaa gctatccaga acatgaatgt tgccctcgga
    16321 ctttctgaga cagcttctct aactgctgtg ccccaatttc cctagtagac accatttaaa
    16381 aattagttat cggttattgg aaaaaccaat ctatgaagat caacgtttca tcctgcattg
    16441 tgtgtattat ggcttgatca ttaatctgga tcatctatat tttcagattt gtccggacaa
    16501 tcatagatca tgcagattca ggtaacaggc cccttgtttt cacggttaaa cctgactcat
    16561 aaactacagt tctccgatca tcgtgatcat tattttgggt gacaattgat tgaaacatta
    16621 ggtctcagcc tgcaaattat ctgcaattct ctctatgtca ggtcttgttt catcagatca
    16681 ctaatgcaga catcttcaac gctctttgtt gctgccatgg ccgcccgcca tgctgcatcc
    16741 tcttctgatt tttgttcaca actacaggcc ctgcgttcac aatgtacaca cttcctgcca
    16801 tacagcctcc tcccaatttt tttaccttta aagacttata tgttgttagt accaaaaatg
    16861 atgtgtattt ggacgttttc agcaaaagta aaacctcatt cattgatttg cattgaacac
    16921 acgagagcta attgctataa ctatttcgtt cctgattgac catggcggac aaaaacatta
    16981 tacacgtctc tgatttgacc aagaccttca acggatttac tgctgtaaac catatctcgt
    17041 tttccgtagc agcaggggaa atattcgctt ttcttggacc gaatggagct ggcaaatcaa
    17101 caaccatcaa tatgcttaca acgttgctcc gtcctacgag cggcagcata tctctaaacg
    17161 ggtatgatcc cgtcacatca ccccaccgcg tccgtcagag ttttggcatc gtttttcaag
    17221 acccaagcct tgatgaagat ctgacagcgt gggaaaacat ggattaccac ggcattcttt
    17281 acggcatatc aagaaatatt agaggcaaaa gaagtgagga actgctgaag attgtggagc
    17341 tgtgggagag aagaaaggaa caggttaaaa aattttcagg tggtatgaaa agaaggctcg
    17401 aaatcgctcg cggcctccta catcatccaa aaatcctctt tcttgatgag cccacattag
    17461 ggcttgatcc tcaaacacgg agtctcatct ggaattacat taaaggtctc aaccagtctg
    17521 aaggaacaac gatctttctt actacgcatt acatggagga ggcggaacgg gttgctcacc
    17581 gcattgctat tatcgaccat gggaagatta ttgtccatgg aacccctgat gagctgaaag
    17641 ctcagacagg aactagctct ctcgaagcag cttttattgc cctcacaggc cgggacattc
    17701 gtgatgaaga accgaacaat cttgagcaaa tgagaatgcg taggaagata tttaggagac
    17761 atcaacggtg aaagtcattt acatactctg gctcagacaa ctgaaacgat attttcggtc
    17821 gaaagcacgg ataattggtt cccttggaca acccctcctt ttctttgttg cccttggctt
    17881 tggttttgga ccaatataca cgagagctgg tggaggtgat tatatccaat ttctggcgcc
    17941 gggtattatc actatgagca tcctttttac agctacgttt tcagggattg aagtgctctg
    18001 ggacaggagg tttggttttc ttaaagagac actggtggcg cctgtctctc gccttcagat
    18061 tatgcttggc cgtaccttag gaggggctac tgttgccgtg gtacaaggct tgattgtctt
    18121 cattctttct gtcattgttg ggttcaggcc tacaaccgtg tccatgattc ctcttgccct
    18181 tctctatatg gcactcattg cgataacctt taccgctctc ggcacggcta tagcgtcgtt
    18241 catagaggat atgcagggct ttcaaatcat tatcaatttc ctgattatgc ccctattctt
    18301 tctttcaggc gccttgttcc cccttgaaaa tttgccaaaa gctgtggttg ttctatcgat
    18361 gataaatccc ctcgcttacg gtgttgatgg acttcgcgga accctaatca gtatggtcca
    18421 tttcggcctt gccactgact ttgttgttct tggctgtata gcggtgtttg tcctgcttgg
    18481 cggggcatat cttttttcaa gaatagaagt ataattcaaa tttagtgaaa aaacgaaaaa
    18541 ggtttattcc cagtatgtga ttcgaacctg gctcaaattt ttagcttttc atttcacctc
    18601 ttttactgat tccctgttaa ttccattgac tattacccat ttttctatta ttctagtaga
    18661 ttatgtttga gaaactgcag gagcgccttg aagccacttt taaaaaaatt aggggctatg
    18721 gaaaagttac agaagataac atcaaagact cccttcggga ggtccgaatt gcccttcttg
    18781 aggctgatgt caattataag gttgcaaagg acttccttga aaaaatcaaa gaaaaggcta
    18841 tcggggaggg tgttctcacc agtatcacac ctggccaact tttcgtcaaa acagttcatg
    18901 atgaactttg tgaacttctc gggcaggtaa acaaacccct cgatgtttca gggtcacctc
    18961 ccgtatcatt aatgctcatc ggcctccagg gctcaggaaa aacaacaact gctgggaagc
    19021 tcgctcttca attgagaaaa aaaggcagaa aacctctact ggttccttct gatgtgtacc
    19081 gccctgctgc catagaccaa cttttaaaaa ttggtcagca gataaatatc gcaacatttg
    19141 caccaaaaga tattcgagat cccatcgtga tctgcaaaga agccaggatt catgctatga
    19201 aaaacggctt tgatacgatg atcatcgaca cagcgggacg tctacatata aacgacgaaa
    19261 tggtagagga actggtaaaa caaaagggct tgctgaatcc ccgcgaaacc cttcttgttc
    19321 tcgatgccat gacaggtcag gatgcggtta ccagcgccac cacattcaat gaaaaagttg
    19381 gcgttgatgg cataatcctt acaaagcttg acggtgatac ccgcggtggc gctgcgcttt
    19441 ccattaaggc agtaacgggt aagcccatca agtttatcgg tgtgggtgaa aaattggatg
    19501 ccttggaacc atttttcccc gaaaggatgg cctcccggat acttggtatg ggtgatgttg
    19561 tatccctagt tgaaaaagcc caagaggttt ttgacgaaaa acaggcaaaa gaactggaga
    19621 gaaagctcag aaaagacgag tttactcttg aagacttccg cgatcagctc aaacaaatga
    19681 agcagctcgg atccatcgaa tctatcatag gtatgattcc ggggttaaat aaattaaaag
    19741 gtgcaataga ctttactgct gcagagaaag acatcaggaa gacagaggcg atcatcaact
    19801 caatgacggc aagagaaagg gtttacccga acatcattga tggcagcaga aggatgagaa
    19861 tcgcaaaagg aagtggtaca caggttcatg atgtgaatga tcttttaaaa aagtatatgg
    19921 aaacacggaa gatgattaaa aagatgacaa aagggggcat gaagggcctc cagagacaat
    19981 tactattcag atgaggaggt gggtaatttg gcggtaaaat ttaggttgtc aaggcatggt
    20041 tcgaaaaaga gacctttcta cagaattgtt gttgctagta gcaattctcc gagagatggc
    20101 agattcatcg aaagggtcgg tttttatgat cctttgaagg agcccatggt gattagcctt
    20161 gacagagaaa aggtgaagaa ttggtataaa aggggggcga aacccacgag gacagtcgag
    20221 aatctcttca aaaaagaggg tgtgttgagt gaaataacac aataacttat ggaggagggg
    20281 ccgtgttgaa agaactaatc gagtacattg caaaggcatt ggttgacaat cctgatcaag
    20341 taaaggtttc tgagattgaa ggcgaaaaaa cctctgttat agaactcaat gtttccaaag
    20401 atgatcttgg caaggttatc gggaagcagg gcaggactgc gcgggccatg aggactattc
    20461 tgagcgcagc ctcaacaaaa gttaagaaaa gggcggtcct cgaaattatt gagtaatgcg
    20521 gtttatacct gtcggtagag tcattgccgg ccacggtctt aatggagcag taaagtttca
    20581 gtattacaat gaggatgtag atcagtttga tttttacacc tcattgtatg ctgacatagg
    20641 cggaagacag acaaagttta acctaacgga ggttagattc agaaaaggct tcttctatct
    20701 aaagcttgaa gatttaaata gcccggagaa gatttccttt ctcatcaacc aggagttgag
    20761 tgtaagggaa gtggaccttc ctctgttaga taatgacgaa tattatgatt atcagctaat
    20821 tgggcttaat gtggttaatc ataaacatga gcctgtcggc gtggtaactc aggtaataca
    20881 cacgggcgta aatgatattt taacggtgac aggcagggaa gaaatattgg tacccatggt
    20941 agaagattta atttctaaaa ttgatcttca aaatgcactt attgttattg atgaggatac
    21001 cttacgttta tgattttcac cgtgctaact ctgtttcctg gcattttcga ttcacctctc
    21061 agggagagta tcacaaaaaa ggcagtcgaa aagggactta tcagcttcaa cattgcaaat
    21121 atcagggaat atgctgatga tgcccacaaa acctgcgatg atgcaccata tggcggtggg
    21181 ccagggatgg tgatgaagat tgaacccatt taccgtgcaa tggctcattt ggaaaaaaca
    21241 atgggaagac cccgatatat cttgctttcg ccacagggga gaatttttga cgaggagact
    21301 gcaatccgat attcacacat caaacacctg tgccttgtat gcgggagata tgaaggtctc
    21361 gacgaaagag tccttgacct ggttgacgaa gaggtttcca ttggggatta tgtgttgtca
    21421 ggtggtgaaa taccggcact ggtcttgatc gacgccatca caaggtgcat tcctggtgta
    21481 ctcggcaatg agcaatcgag tgtcgatgag agctttagca gaggactcct tgaatatccc
    21541 cagtatacaa ggcctgacgt cttcatggat atggaggtcc caccagtgct cctatcggga
    21601 aaccacgagg agatcaggaa atggaggaga aaggaagccc taaaaaagac gatttttaaa
    21661 cggcctgacc tcatgaacag gtttacccca accgatgagg acaggaagat gattcgagag
    21721 atcatggagg aaatgcctgg ataatgtgag tattgcaata attcattatc ccgtttatga
    21781 caaaaatggt gatattgtgg ctacgagtgt aacaaacctg gaaatacatg atattgcaag
    21841 aacatgtatg acatttgggt taaaattatg ctatattgta acacctttgg cgaaacagag
    21901 gacaataatg gaaaagctga tccatcactg gacaaatgga tatggctctc gttataatcc
    21961 tgcaagggca gaagccctta aaaaggttgt gctcaaggcc ggagtagatg agatggttgc
    22021 tcactgtcag ggaaaaccca tacttgtggg cacatccaca cgggaacgac ctggtaaatc
    22081 gattgcctac cgggaattac agattctgat agaaaaagag acacgaccgc tcctgctcct
    22141 cttcggaacg ggttggggtc tatctgacga agtggttgaa cgttgcgaga aaatgctcat
    22201 acccataaga ggtaagggaa attacaacca tctctcctta cgggtcgctc ttggcataat
    22261 tctcgataga ttatttggca aaagaggagg atatgatgaa tgagatgatt gatctacttg
    22321 aaaaagaaca tatgcggctc gatttaattg agaccagtgt tggcgataac gtaagggtct
    22381 atacaaagat ttttgagggt gataaagaaa gaatccaggt ttttgagggt gttgtcatca
    22441 ggcggcgggg aggtaacaca cgtgccacat tcaccgtgag aaaggtttcc tatggtattg
    22501 gtatagagaa aacctttccc atgcactccc ctctcatcga caagattgaa gtcctgagca
    22561 agagcaaagt aaggcgatca aggcttttct acttacgaaa cctcagaggc aaggccgcaa
    22621 agttgaaaga aaagcgggat tgatggtttg caccctgact ggactcatag gcgggattga
    22681 tgaagcagga agaggacctc tcgcaggtcc cgtcgtatcc tcctgtgttg tctggcgcag
    22741 cttacctgat caccccaaaa agattaatga ttcaaagctt ctctctccaa aacagaggtc
    22801 agatcttttt ccatggattc agcagcatgc attcaaaata ggcattggta tcgcaaccca
    22861 cgaggaaata gacaaaatca acatcctcag agcaagcctc ctctcaatgg aaagggcgtt
    22921 gcataattca ggggttgaac caaaccttgt tcttattgac ggcagattcg ctctccaatc
    22981 ttaccctcat agcagacctg ttgtgaaagg agacagaaaa tgtttttaca ttgcctgtgc
    23041 gtctattgtc gccaaggtta ccagagatac catcatgaac agatatgatc ttgaatatcc
    23101 ccagtacaat ttcagaaaaa acaagggata tcccacaaaa gaccacagac tcgctattaa
    23161 acagcatggc gtctcgccca tccatagaaa aacctttaag ggtgtgaggg aattccttgg
    23221 cgtctaaaca ggaagaagga agggaaggtg aaaagcgggc tgaaaatacc ttgaaaaggg
    23281 caggttataa aatcattgaa aaaaacttca ggagccgatt tggggaaata gacattgtcg
    23341 ctgaagaaaa cggctgcctt gtttttgttg aagtaaaaaa acgaaacacg ccaaattttg
    23401 gtgacccatt caacgctatt aatgccagaa aacgacaaca cctgattaaa tgtgcgatgt
    23461 tctatttgaa aagtcataat tcttttaaca gaaaaacgag atttgatgtt gtgggcatag
    23521 agggagaaca agtaaaaata atcaaaaatg cctttatagt agaggagtag acaaaatagt
    23581 ggaacggaaa aagtggaagt aagaacaaga aaaacacgtt tcaagtatta ttccttttca
    23641 catttcagtt cttacttttc actggtggaa tccatacaca tgtgcgacgg cgaatgtcat
    23701 gacacataaa aaaattttga aacttcttga aagtgtaaag aatggaaaaa caactgttga
    23761 acaagcgtat gagagactca aagacattcc ctttgaagat ttgagccata caaagataga
    23821 tcatcacagg gttgtaagaa aaggtcttca agaggtaatc ttcggggagg gcaagagcat
    23881 ccatcagatt aatgatatta ttcgcgccat gcagaacaaa ggcctcgata ttctcgtaac
    23941 aagggtatca aaagaaacag gcagcgaact gactgccctt tttcccgttg gagtctacaa
    24001 tagcgatgca cgatgctttt atatcagaaa ggacctatct gttaaaggca aaggatccat
    24061 tctcgtcatg tctgcaggaa cgagcgatat ctccatagct gaagagacat atattacctc
    24121 tatttttttc gggaataaga ctgaacgcct ttacgatgtt ggggttgctg gaattcacct
    24181 gctcatcgag aacatggatt tattgaagca agctcgcgtc attgttgttg ttgccggcat
    24241 ggagggggca ttgccctcaa tagtagcagg catggtaggg gtaccggtta tcggcgtgcc
    24301 cacaagcatc gggtacgggg caagctttgg cggactttca gcccttctcg cgatgctcaa
    24361 ctcgtgctcc acagtctctg tttttaatat tgacaatggc tttggtgcag catattttgc
    24421 aaccctcatt aatcgtttat gaacatactt tttattgatc ccatctttgg catcagtgga
    24481 gatatgctaa tcagcgcttt ccttgacgca ggcatgcctt tccaggaact ttacgacttg
    24541 cttgcaaaaa tccctataga tatgccagaa ataaaacctg taaaaatgaa ccagggcatt
    24601 atagagggga tacatttgga aattgcagag tcaaaaactc atctctcaat cacacaaatg
    24661 gagcaactca tcgatgactt gaacgttgat tctgagataa aggatggtgc gaaaggtatt
    24721 ctcaatgttc tcgttgctgc tgaagccaag gttcatggcg ttccaaggga ccaagttcac
    24781 tttcatgaac tttcccacat tgacacactt atcgatgtga tttctgttgc aaagtgcata
    24841 cattatttcg acatcgaaaa ggtgtattgc gggccattgc ctatgggtag gggaactatc
    24901 aaaatctctc atggctatgt acccaatcca cctccggcaa cggtggagat tctgacaggc
    24961 tatagcctta gatttattga tacatcactc gagctgacaa caccaaccgg agcagcagta
    25021 gtaaggtact atgtcaaaga caggcatgga atcccttcct tcacaatgga gaaaatagga
    25081 tatgggctcg gatcgtacaa atcagaaata cccgatgcac tgaggatttt tatcggtaga
    25141 agcgaacacc cttcaggtgt ggaagaggtc tggcttcttg aggcagatct cgatgatatg
    25201 aatatggagt accttggagc tgtagctgaa agaatccgag aaaaaggagc cctcgacgtt
    25261 ctctattttc ctgtctacat gaaaaaaggc agaattggga tacggctttc tgtcactgtt
    25321 ccggcgaagg ctttgcagag tgcgatcgat tcagtcttta tggagacaac taccttcggg
    25381 gtaagaatgc aaaaattgca gagacaggta ttgaaaaggg acgaaaagat catccatact
    25441 ccatacggat ctgtaagggt aaaaaatggc tataacagag acggacatct agtcaaaaca
    25501 cacattgagt ttaatgatgt taagaaaata gcagacgaca aaggtcttgc ttaccagtct
    25561 gtcctggaga ttgtcaaaag aaagttagac tccatataat gaaggtcagg ggttatcaga
    25621 cctgaactca agtaggtttt tttaatgcct ctggacgatc ttgatcttgc tattgttgat
    25681 gttgaaacaa cagggacagc atctcactac caccgtatta ttgaaatagc agttctgagg
    25741 attcagtccg gacacctggt cgacaaatac tccaccctcc tcgatcccga aagggctatt
    25801 cctccattca tcgaaactct aaccggcatc aaaaataaag atctcaagaa tgcgcccacc
    25861 tttcgtcagg tgaaaaacca catctataac ctgcttgatg gggctatatt tgttgcccac
    25921 aatgcgaaat ttgattatac cttccttaga gaagagtttg aaagggagaa gataaactac
    25981 acagcaaaaa ggctctgtac catgagactt tcaaagaccc tctttcctgg cctgaaaaag
    26041 cataatcttg acagcataat cgaacggttt ggcattaaat gcagtgacag gcacagggct
    26101 gaaagtgacg ccagcgccct atgggatttt cttcaacgag taaaggaaca ggtagacaca
    26161 gagaacttca ggagaattct gggcaagatt ctgaaaacac ccaccctccc gccccgcctg
    26221 aatgactcca tcataaaatc gcttcccgaa acatacggag tgtacatctt ttatgatgaa
    26281 aacagtaatc ccctctatgt aaacaagagc gtaaacattc gcaacagagt actttcccat
    26341 ttatcagaag accaagaatc agcgagagaa caggcactat cgaggcgagt ggtcgatatt
    26401 caggccattg ccacctcagg cgagcttggt gcactcctcc tcgagtctca cctcatgcag
    26461 caactgtcgc cactgtttaa ccgcaaagca agggttcata aaaagctggt ggcactgaaa
    26521 agagatctaa atgaacaaaa atacgggaca atccgccttg agcatctgaa caacctccga
    26581 cctgcagacc tgccaaacat agtgggcata ttcaagtcga ctcaacgagc aaaagagttt
    26641 ctctggaaca tcgcaaaggg atataccctg tgcccacgca tacttggtat agaaaaaggg
    26701 aagggggaat gtacatacag ccagttacag aggtgcggag gagtctgcag tgggcatgag
    26761 tcagccaata cctacaatac aagatttaac ctcgcatttg ctgggagaag aatggtaccg
    26821 tggcctttta gagggcctgt tctcattgag gaaaaaaatg tatcagggaa cggaggggag
    26881 tattttgtcg ttgataactg gtgcctcttg caatacttca ggtatgatga aacaggcacg
    26941 aaacaatgtt tgcttaaagg taatggtagt tttgactatg acgggtataa gatcttgaga
    27001 gattatcttt tgaattcgag gaaacaaaca aacgtaaggc ctctgacgat aagtgaattt
    27061 accagtatct tagagaatga gggacagttt aaagttgttc cacggaaaat caaacagagc
    27121 tgagtaggca cagcggttgg ctaatcattc aggggtcttt tcacaggtct ttctctgaac
    27181 cgcggactga atccaatcag ttaaaccatt caacttcagt cttaggatag catctttctc
    27241 tcattcgggt atttcagttc ttacatcact atttaaaaat gcttcatttt ggtaaagggc
    27301 aggggaaaag aaaaacgtag gtcaaaaggt ctctccgctc cccctcccct attttttaat
    27361 tgtctggcct tatccttttc ataaaattct ccctcacagc cctcaattct tcaggggtcc
    27421 tgcaaatctc ctctgcctgg atagcctgca tattttttag aattgcaaaa ccaaacttat
    27481 cactgtaatc agtctcgccc cacaccacgt cctcaacgac agcatggtcc tcttttctca
    27541 ttatcttcca gccctcaggc gtttcccacg ccaacggttc cttttcaacg gctgtaacga
    27601 ttttgtcagc attgagtgtg cccgctctct cgatagcagc ctttatgaaa tacacacccg
    27661 cataagtctc tgtcgccatg tagtctggta attctttgaa tcgttcttgg tacatcttaa
    27721 caaatttctt atttatagcc gtatcaggtg taagaaaaaa ataacgggca gccacaaata
    27781 taccattggg catatcctta ccgagcggta taagggtttc gagggctgca ccgtcaggga
    27841 aggcaaaagc gatcttgtca aacagttgct tttcctttgc ctgtttaata aagttgacag
    27901 catccatacc ccaaagaggt gaccagactg catccggctt tgcctcaaca atcttttcaa
    27961 tgaaaggttt gaactccttt tcagtcagtt ttggccacgc ctcgcctacg aactcaacat
    28021 ctgggcgcag ctctttcagc ttttctttga atgctgccca cgattcatga ccgtagttgt
    28081 aattgggacc aataaccatc catcgcttgt aaggcttgga ggccatgtaa taggctcctg
    28141 agcgtgagtg catcatggcg ttgcttaaag tggcaaactg gtagcgccca aatcccgtat
    28201 ctgtcatgga gtttgctgca gcctgggtga gtacaataat tttctttttc tcattagcaa
    28261 gtttcgcaac tggcacggca acaccacttg atgtcggacc aataatgaag tcaactttat
    28321 ccttttcaat aaaccgcttc acgatctctg tggccacatc agctttcagc tttgtatcct
    28381 catagatggc ctccaccttg cgcccaagga tacctccgct ggcattgatt tcatctacag
    28441 ccatctgcac tgactggaaa ccgtgcttgc cgtatccgcc catgggaccc gatgctataa
    28501 agagcactcc gatcttgatc ggtttcgttt tcgcagcctt ttccgctgca aaagatgtcc
    28561 cgcaggcaag caaaatacct actagaatca atacaagcgt aaccagtgat ttttgttttg
    28621 tcataattac cccctttaat tttaatattt caatataccc tgtctcacat aggatttcaa
    28681 acactataga cgatgatttt tgaggttggt ttttgtggaa accgtggcat cattgcaaga
    28741 aaagctacca gccaggatac aaggggagat atataaagcc ttaggtggca ggacatgaaa
    28801 ggccaaattg ttgcatgtat tccggcgttt attttttcag acacatccat tgtgaggtca
    28861 atcaacattc gtacgcatgg cctgtgatcc taattgcctg tacaatgcaa aaaatactct
    28921 taaagggtgc atcgccatgt acaaagagga attccgaaca gcaaaagatg gttatcgcaa
    28981 caaaaaccat gtatgcgcag aaggatacag ggaaagctgt gaatcatgcg ttactgaacc
    29041 aattgacgta cttgccttat gcaaggcacc atgcaaaacc aggcttgatg aagcagcagc
    29101 caactgtcgt ggtgcatttg ccaaaggaac cccggagcgc gacatgtgta ttgaccacgc
    29161 acaagtagtt acttttcaga gtagtgatga atgttgagag aaatggcaac catccttgaa
    29221 agcatgcagc tataccttaa agacatgaat gatcggatgc aagccacaac cgccatcagc
    29281 gccgtaacca tagcgcaggc gccactcaaa caccggcgct cacagggccg ggaatatcgt
    29341 catcgaatta atgtttgctc tgttttcttt gtaattagat gggctcagaa aaacatctcg
    29401 aagcggtcat tcgctgcctt gcagatagga caagtatcag gtgcctgctc acgggcacaa
    29461 aggtagccac acactttgca cctccagaca ggataggcca aattcttttt ttgtcccagc
    29521 gagtccaacg ggctcaatgc tttttttctt gagataaaaa tcgtctttca gggacaggct
    29581 caaacttttt ttcacccctt gccacggctt cagacacata aagggcgcaa taacatgagc
    29641 cgaattcact cacatccagg tctcgatagt cgcagggaca gataatatcc atatccttcc
    29701 atttgtcgcc ttcggaaagc ctgcaggggc acgagttgta gccatatcgt ttctgattta
    29761 ctattagtcc cttgataagt tctgttacga aagcttcgtc cgtatttaag ttgtaccctg
    29821 atgcctgtgc atcttttttc agtttatcaa ataattcctg aacttcatct ggcattatgc
    29881 tctccgtgca gttcataccg tatctatcgc tttctttatt tcatcctctt tgtagccaat
    29941 cacacatttc ttgccatgaa caaccagaga gggaaatgaa cacttggggt tccaccgctt
    30001 cacttcttca agggcctgtt ctttctcctc accctgaagt tggtcaacat agacatagtc
    30061 aaaaccaata ttcatttttt caagaagtgc ttttgtcatt ttacaccaga cacatgtgct
    30121 gagggcatag agctgaatgc tccccctatc ttctccatca acgcgttgtt tagccatgtt
    30181 tcacctctgc tgtatttgta cacttttatt tacttttaca tctgtgataa tagtcttagt
    30241 ctgtgagtta gc
//
LOCUS       DAOOMC010000019        29389 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdus sp. isolate AMR_MDS_2685
            NODE_1381_length_29389_cov_5.536238, whole genome shotgun sequence.
ACCESSION   DAOOMC010000019 DAOOMC010000000
VERSION     DAOOMC010000019.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864760
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdus sp. (wastewater metagenome)
  ORGANISM  Syntrophorhabdus sp.
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae; Syntrophorhabdus.
REFERENCE   1  (bases 1 to 29389)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 29389)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,729
            Genes (coding)                    :: 2,708
            CDSs (with protein)               :: 2,708
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 7 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29389
                     /organism="Syntrophorhabdus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1381_length_29389_cov_5.536238"
                     /isolate="AMR_MDS_2685"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2699756"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..771)
                     /locus_tag="PK125_03290"
     CDS             complement(<1..771)
                     /locus_tag="PK125_03290"
                     /inference="COORDINATES: protein motif:HMM:NF013714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HPB37164.1"
                     /translation="MIKESVIAELQKIVGKENVKTEKETLKAYSYDGTTSWEHEPDVV
                     VFPTTTKEISEIMKLANKEKIPVTPRGGGTNVSGGSIPIMGGIILCTTKMNKILKIDK
                     ENLTATVEPGVVLQDLTIRLGKEGLFFPPDPQSFLGATMGGIIAENAGGPACVKYGVT
                     KQYILGVEVVLPTGEIVNLGGRTLKNVVGYDLLHIFISSEGTLGVIIKAELKLNPLPQ
                     ARKTIMGVYDDVAKAGENVFRILENGVIPGKIELLDNWV"
     gene            1007..1915
                     /locus_tag="PK125_03295"
     CDS             1007..1915
                     /locus_tag="PK125_03295"
                     /inference="COORDINATES: protein motif:HMM:NF015509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HPB37165.1"
                     /translation="MAIIETIVPIFLIIIFGFILQQRGLLKREFIQEANRFVFHFSLP
                     VLIFIGIIKSDIREVSLTSILSVIVPTLVILCIAFLLALAMGLKKGTLGSFVQTTFHG
                     NITYIGLAVLYYMMGEEGMRRGSILIGFLILMNNTLAIAILSWASRRHENIWKSLASV
                     VKSPLIIGTVLGMFLLYLDIPVPILLMKTMGILANIALPMALIIIGASMSMNTIRNSF
                     KLSGIVVFMKLMVLPSLSFAFCHLYAIPFTDALPGIILLATPTATTSFILANQLGGDT
                     DLASGVITLSTLLSPFVFIFWAYAAR"
     gene            complement(2217..3176)
                     /locus_tag="PK125_03300"
     CDS             complement(2217..3176)
                     /locus_tag="PK125_03300"
                     /inference="COORDINATES: protein motif:HMM:NF013356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HPB37166.1"
                     /translation="MPIEVVITNDFEHLSKVAAEIVKKKLVELLKRKKEVVLGLATGN
                     SPTGMYKHLARAANNGEFDSSRIRSFNLDEYIGLPGENAQQRALHPESYCYFMIQEFF
                     GLLKKKFIETNVPFGSLIDQKVLIKELKRYPHDWACKGTGAGKSIVIKQKTGSEYLSW
                     IRKEILNGYMQKIKKAGGIDIQIIGVGGRGHVAFHESGVPFEGSSVMLVKLDDNTIKD
                     SVTDGHFISKKDYPNYAISMSAELVYQARNVVLLANGERKVESVTRSLLGDVNPEVPI
                     SYGQKYALNGGNLIYVLDKIAGRNLIDNKSNLKKMNIVLKDLT"
     gene            complement(3224..4573)
                     /gene="glmM"
                     /locus_tag="PK125_03305"
     CDS             complement(3224..4573)
                     /gene="glmM"
                     /locus_tag="PK125_03305"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: protein motif:HMM:NF008139.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HPB37167.1"
                     /translation="MGRLFGTDGVRGEANRYPMNAEIAFALGQAVVYILKKEHGRPRI
                     MIGKDTRISGYMLESSLESGITSMGGYPYLLSVLPTPGIAFIAQSMRAAAGIVISASH
                     NPYQDNGIKIFSGSGFKLSDEQEETIEDLMLSNTLHTLVPPVKDMGQAFRMEDVNGRY
                     IVFLKNTFPRDLSIEGMKIVLDTANGATYKVAPDTFWELGANIDVIHNSPNGININDK
                     CGSQHTEDLRKRVVEKGAAIGLAFDGDGDRLIAVDEKGQEITGDQILLICAKMLKDQG
                     KLKNNLLVATVMSNLGLRVACKRYGFKYHASKVGDRYVLEDMIRLGSIIGGEQSGHMV
                     FLDHHTTGDGIVTAMQLLAAMVKTGKPLSELAKMMDIYPQKLINVDVKSKPDINTVPK
                     VMKIIDKVERELKDEGRVLVRYSGTQNMCRVMVEGPSDSVTEKYCEEIADVVKNVLG"
     gene            complement(4605..5858)
                     /locus_tag="PK125_03310"
     CDS             complement(4605..5858)
                     /locus_tag="PK125_03310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB37168.1"
                     /translation="MDIKLRCHHKIVQMIDKGVDIPNPFSIDVGHEVDVNHISGQGVK
                     IYPGCRIYGEKTVISNGCQIGYEGPVTIDDCQLGSMVELKGGYFNKSVFLEKASMGLG
                     AQVREGCILEEEANGAHCVGLKQTILFPFVTLGSLINFCDCLMAGGKSRKNHSEVGSS
                     YIHFNFTPDGDKSTASLIGDVPRGVMLNQPPIFLGGQGGIVGPLRMGYGNVVAANSVL
                     RNDFPEDNKLIVGKSYSGKTVNFKPRAYQGLSRIVGNNIIYIANLMALEQWYIHVRHS
                     FLESQEFGQQIFSGLLEKLALAKKERIKRLQVLAEKSKITLKNESEEGSDTVGRNEFH
                     ERFSDIERLFTEGICDDSVERYYDDFFFEFERLRGKKEAKYIAVIQGLPAHVSKKGVL
                     WLQKIVDIFCERVKAIVPSFKLVGC"
     gene            6205..7395
                     /locus_tag="PK125_03315"
     CDS             6205..7395
                     /locus_tag="PK125_03315"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014414.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane-fatty-acyl-phospholipid synthase
                     family protein"
                     /protein_id="HPB37169.1"
                     /translation="MKEVLRKLLRTLSHSLPEIPFEIRYWDNQSERFGTGEPSFTIIF
                     KHQKSIKKIFSKGSMGFAEEYVEGNIDVEGDFVYLVRLGICPEFQNIKLSFGTRMAAL
                     MNHLLSLNTLRGSARNIAHHYDLGNEFYRLYLDESMTYSCAYYRNKNDSLEDAQQQKY
                     EHICRKLALKEGETLVDIGCGWGGMLLYAVHHYGVKGLGCTLSVHQADYAKKRVAEEG
                     LEKSITILLEDYRNVKGQFDKYVSIGMFEHVGKGLIPMFMEKTKSILKPGGIGLLHTI
                     GKERDTPFDPWTMKYIFPGGHLPALDHIIRTMGDKSLIPVDIENLRLHYSATLDEWAK
                     RFEKNSDQIKKMFDERLVRTWRMYLRGSSAAFRWGDIRLYQILFTNGLNNSLPMTREH
                     LYKS"
     gene            7551..8771
                     /locus_tag="PK125_03320"
     CDS             7551..8771
                     /locus_tag="PK125_03320"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HPB37170.1"
                     /translation="MHNILKSNKLDHVCYEIRGPLLREAKRLEEEGYTIIKLNSGNPP
                     AFGINAPDEIIRDVILNIRSAQSYGDAKGLFAARKAIMQQCQLTGIEDVEIEDIYVGN
                     GVSEMIMMAMQGLLNNGDEMLVPSPDYPLWTAAINLSGGKAVHYITDESSDWNPDIKD
                     MESKITAKTKGIVVINPNNPTGAVYHRDILIKIIRLAEKHNLIIYADEIYDQILYDGA
                     KHIPLASLSKDVLFVTFNGLSKSHRIPGLRAGWMIISGKKQVAYDYTTEGLDTLSNMR
                     MCGNMAAQFAIQTALGGRQSIIDLISPGGRLYEQRKVAYESFKAIPGISCVKPMGALY
                     LFPKIDVKKYKIYDDQKFLLDFLLEKKVLMVQGTGFNWKKPDHFRVVFLPNVEEIRTV
                     AMHMADFLKEYRQI"
     gene            complement(8966..9784)
                     /locus_tag="PK125_03325"
     CDS             complement(8966..9784)
                     /locus_tag="PK125_03325"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HPB37171.1"
                     /translation="MKFGFSTFFFIQKSINEVIDDIISSGVRTIELSLETPHVQKMDK
                     DFVEQMAALAGDGLEFSIHAPFFEVNLGSHQPEIRQFSRKRVKMAIDMAYAIGANPVV
                     LHPGYTFWMGKIKDVAEKSWKFFINDMRSILSYARKKDVTLALESIPMHLFFFYDLPE
                     FKELKDVFPDLGMTLDTGHAFLTKIAKKEKKPEEAIISDLKYLGINNITHVHLHNNKG
                     KRDDHLFPDGDMDIKRMLNFLREEGYSGKVIIESYEMEKNGIPSVLEKLRSMIS"
     gene            complement(9903..10547)
                     /locus_tag="PK125_03330"
     CDS             complement(9903..10547)
                     /locus_tag="PK125_03330"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: protein motif:HMM:NF001380.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HPB37172.1"
                     /translation="MRIVLLGAPGAGKGTIAKLLSDFDGSVQISTGDILRNAVKAKTK
                     LGLEAQGYMERGELVPDRLIMDIMEARLQEPDCAKGYLLDGFPRTIPQAEELKKLLTK
                     LNMKLDAVINIDVPKEVILDRLTTRRTCSNPDCQEIYNIKSKPPSPDGKCLKCGSPAV
                     QRADETVEAITKRLETYNEKTAPLIDFYAKEGLLKTIASLSSDEIVSQIKEAVK"
     gene            complement(10806..11225)
                     /locus_tag="PK125_03335"
     CDS             complement(10806..11225)
                     /locus_tag="PK125_03335"
                     /inference="COORDINATES: protein motif:HMM:NF024865.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HPB37173.1"
                     /translation="MKADSETEADVMDVLGRFIRAYNDKDMDGILNLFASDPDVVFYG
                     NGKDEKSIGISGIREQAQHDWGQDASISLEVQWSSVSSAGLVAWLATDIQIHAEARGM
                     EMVMPARLTAVLEKRDDRWFFMQWHTSLPTVEEPEND"
     gene            complement(11284..12078)
                     /locus_tag="PK125_03340"
     CDS             complement(11284..12078)
                     /locus_tag="PK125_03340"
                     /inference="COORDINATES: protein motif:HMM:NF022789.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3147 family protein"
                     /protein_id="HPB37174.1"
                     /translation="MDNIFVLKLILSFIVGSLWITMVTILAEKYGTRIGGLMAGLPST
                     ILISLFFIAWTQSERVAVDATTIVPIIGGINCIFIVAYIFLLKINFWVALGGAFLIWT
                     LLSFGIVLINFNSFPVSAISYACLVLITHYIVEKVLKVRSKAGRQMQYTLSIMIFRGV
                     LGGLVIVSAIIVTKIGGPLIGGMFVMFPAMFVGMIFMTYFSHGTTFSAAVMKSSILGA
                     ISVVIYGIMVRYTYITLGFIGGTVVSMAVSFASSCVIHRYLMRKTS"
     gene            complement(12561..13724)
                     /locus_tag="PK125_03345"
     CDS             complement(12561..13724)
                     /locus_tag="PK125_03345"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPB37175.1"
                     /translation="MQRYHNLQGKALLFLFFLWFIWFTNFTTRIAFAPILPLVEDEFL
                     VSHAKASSIFVFLSVGYGAAVIVSGLFSGRFGYKRSIVFSLLLLSLVAFLIPLVHEFR
                     ILYFFAFMLGFSVGFYLPAVIPLITEYYAEKDWGKAIAIHDTGASCSIFATPFIALFL
                     LHFFPWRGIFIVLACVYVICSVVFLMTSSEIKVNDPPKAVFTSIMKIKSLWFMAVIWI
                     FGAGANLGIYSVVPLYLTKELKMNIGFANTILGISRLGAIFVAIACGFLIDRFNLRRI
                     MFFVMITTSVFTVLMGLSSVKYIGFVLFLQAFVVTAFFPVGLVALAKTFNREMRSLAT
                     GIILSVSIAFGGGVLPYLLGISGDMVSFRLGITILGIFVGLASTLIFHLKELE"
     gene            13852..14718
                     /locus_tag="PK125_03350"
     CDS             13852..14718
                     /locus_tag="PK125_03350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB37176.1"
                     /translation="MKNDPARWVTKVIEKFNTTSDNSLMNPQNEPAWDKPLVGFANGN
                     DPLWDEYKRHIGDFYWTPHEIFELTFPSLSVKPDELTVISWILPQTKQTRLDQRKENS
                     LPSERWARSRKYGEEYNMKLRLHLVKSFNDEGIKACAPLLSPLWKQMVSTSCGLASTW
                     SERHAAYTAGLGTFGLCDGLITPLGKAVRCGSVIARIAIKATERPYEDPHAYCLYHSE
                     GTCGKCILRCPADAITREGHDKNKCQQYVHGVAHEFIKSKFGFDAYGCGLCQVEVPCE
                     SGIPSKKGIDNG"
     gene            complement(14767..15330)
                     /locus_tag="PK125_03355"
     CDS             complement(14767..15330)
                     /locus_tag="PK125_03355"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HPB37177.1"
                     /translation="MYETIYEMAKPYLNTRYNDVHVELSYEFTKRLLKHYPEADEDIV
                     LPAILLHDVGWKMVPEDEQAGAFGPEVKKNDVQRFHEIEGAKMAGQILNELNYPGSKI
                     HEIMDIIDGHDTRLEALSLNDKIVKDADKLWRFTTTGVNIDSFRFGIERISYINYLEN
                     RIKEWFFTLAAQDMARETLAITRSQLS"
     gene            complement(15436..16215)
                     /locus_tag="PK125_03360"
     CDS             complement(15436..16215)
                     /locus_tag="PK125_03360"
                     /inference="COORDINATES: protein motif:HMM:NF014035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HPB37178.1"
                     /translation="MTRSTKFTIGLASGTLGGFFGGLLGLGGGVIMIPLMTWLAKVTQ
                     HQAHGTSLVAIVFTGIVGAVTYYGLGGVDLKIALILAVSATITARFGALYAHSLPEKK
                     LKVAFGCFLIFASILLVVKGYLPITTWTPGQWTYMVIFLAIGSVTGFVSGMMGVGGGG
                     VMVPLMVVVGNVGQHMAQGTSLLAMIPASMSGASTHYKLGNVNLDIAWGLAMGALVGG
                     YLGALTANQLPDIYLKISFAGVGTWMGMKYIKAGKMMKIEK"
     gene            16384..17043
                     /locus_tag="PK125_03365"
     CDS             16384..17043
                     /locus_tag="PK125_03365"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HPB37179.1"
                     /translation="MTMKKIISFDLDGTLVHGKYGNMVWNHGIPQEYSRKYGMPFKEA
                     EQLIRHQYEEVGEANIKWYHIEYWLKRFDLPVSAKTLLDRFESYIEVFPDTKEVLEAL
                     KNTYTLIVASNAAQIFVQKELSYANLTSYFTHIISATSDYCMVKKEIRFYEKLCDTLS
                     ISPYEIVHVGDHHIFDFETPSQLGIDAYHFVPDHKDSSNQSHTDNGKTIHCLSDLLNI
                     L"
     gene            17073..17318
                     /locus_tag="PK125_03370"
     CDS             17073..17318
                     /locus_tag="PK125_03370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB37180.1"
                     /translation="MLEFISRRDECQVCGSDLHVCLNCSFFDETKSNKCREPQAEYVK
                     EKDRSNYCDFFRFRDDVQKKSGKEEAKKLWDELFKKV"
     gene            complement(17619..18791)
                     /locus_tag="PK125_03375"
     CDS             complement(17619..18791)
                     /locus_tag="PK125_03375"
                     /inference="COORDINATES: protein motif:HMM:NF013347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HPB37181.1"
                     /translation="MANYALIATSSFGLESVVAQELRNLGYEDLIVENGRVIFKGKEP
                     DIARCNIWLRTADRVLIKMAEFKATDFDELFQGTFNVAWEDIIPVNGKMHVTGKSVRS
                     KLASVRDCQTIVKKSVIKAMNRKYHLARFPETGPLYRIEISLVKDVAILTIDTTGQGL
                     HKRGYREESGEAPLRENLAAAIVLLSRWKPERVLVDPVCGSGTIPIEAALIGRNIAPG
                     LKRNFVSESWQNMSKIMWDTVRSEARMKENTTRFRILASDKDGRVLKKARDNAVRAGV
                     GEVITFQRLPVEQFSSHKKYGCIICNPPYGQRIGNSKEVEQLFRSMGEVFSKLKTWSF
                     FILNAHPGFESFFGKKSDKNRKLYNGDIKCYLYEYFGPFPPRKLKITVQKTEDIAQ"
     gene            complement(18791..19525)
                     /gene="truA"
                     /locus_tag="PK125_03380"
     CDS             complement(18791..19525)
                     /gene="truA"
                     /locus_tag="PK125_03380"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HPB37182.1"
                     /translation="MRNIVLTISYDGTNYHGWQCQPDVLTIQEIFNNSLKKILNHATT
                     IYAGGRTDAGVHAMGQVINFHTERTIELRSLIQGLNSILPRDIRVKHAREEDITFHAR
                     YSARSKTYVYCILNTPYNSPFLNSYVWHIHYQLNTSAMDDAAKVIVGEHDFSAFKKKD
                     EVYKSNVREVFRANVKRRGNLIYFVTEAKGFLRYMVRNITGTLVLIGSGKITVEEFIK
                     ILNSKDRENAGPTAPARGLFLRKIKY"
     gene            complement(19624..19758)
                     /locus_tag="PK125_03385"
     CDS             complement(19624..19758)
                     /locus_tag="PK125_03385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB37183.1"
                     /translation="MLKEENAFLKAENVGLKKEVEELYKRLEDHGVTKNLPEDKKPAK
                     "
     gene            19965..22421
                     /gene="gyrA"
                     /locus_tag="PK125_03390"
     CDS             19965..22421
                     /gene="gyrA"
                     /locus_tag="PK125_03390"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HPB37184.1"
                     /translation="MHASVVTVPIEEEMKRSYLDYAMSIIVGRALPDIRDGLKPVHRR
                     ILYAMYELNNTHDKPYKKSARVVGDVIGKFHPHGDQAVYDAITRMVQEFSLRYPLIDG
                     QGNFGSIDGDSPAAMRYTEVRLSRIAEEILKDIEKETVLFRANYDESLMEPVVLPSRI
                     PNLLINGSAGIAVGMATNIPPHNLTEIINGISAYIDNPGVTLDELMEHIPGPDFPTQG
                     IIYGRQGIREAYETGRGHIVLRARANVEVRKKDGREAIVITEIPYQVNKAKLIESIVE
                     LVNTKRIEGISNIKDESNREGIRVVIELKRGEMAIPILNKLYRHTQLQTTFGIIFLTI
                     VNNEPRVLNIKSIIEEFVNFRKEVVTKRSTFELNKAKDRLHILEGLKKALEHIDEVIA
                     LIKKAPNTQEARASLMARFDFSEKQATSILEMRLQRLTSLERNKIIDDFNTTSAEIAR
                     LEQILADEKLLLALVKEELLEIQDKYGNDRLTDIVDALPDITIEDMIKEEEVAVTVTY
                     KGYIKRTSLDQYKNQLRGGKGKIGIVVREEDIVDHLYIASTHSHLFFFTNSGKAISIK
                     VHVIPEAPLTSKGRAVVNLINIDKDERITAICHVKEFSEDKYLFFATKKGLVKRVNLS
                     LFKHLRSSGIRAISLPEDDSLIGVLETDGHAEVILATRKGMSIRFKEEEVRPMGRVAY
                     GVRGIKLKKNDEVVSVENVEQGCNVFTVTEKGYGKRATCTGYRTQARGGSGVINVRCG
                     SKNGQVVCLKKVTENDEVILITDTGRTIRFATKDIPLQNRGGLGVKLMDLQNEKTITG
                     VAIIGEDEGSETEKSENNHG"
     gene            22476..23468
                     /locus_tag="PK125_03395"
     CDS             22476..23468
                     /locus_tag="PK125_03395"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: protein motif:HMM:NF000940.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046167 - glycerol-3-phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HPB37185.1"
                     /translation="MKIGIIGAGAWGTAFAIHLARQGHQILLWVYEPELFDVLQKTRE
                     NTFYLPSFLLNSQIDLTLSLKDFANFSDDVVVAIPSFALRKTLEPVSPLLSGKRILVL
                     TKGFEENTFMRMSTVIHEITQSRVPVASLSGPSFAKEVAHGFFTSVVIASGGNEVSKY
                     FQNIVHNRNFRVYTSEDIIGLEVGGAMKNVMAIGAGIIDGLKLGTNAQAAFVTRSLAE
                     IKRLGKAMGAKETTFMGLSGIGDLILTCYGGLSRNRAFGIELSKGGKPEEIIVNQKQV
                     VEGYYTIKASHSLSKKLDVDMPITSELYKIVFEAKNLQDSIEDITSRGFKDEDA"
     gene            23627..25009
                     /locus_tag="PK125_03400"
     CDS             23627..25009
                     /locus_tag="PK125_03400"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: protein motif:HMM:NF013836.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070569 - uridylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase"
                     /protein_id="HPB37186.1"
                     /translation="MDNRAILDLLQVKNQDHIIHHVDRLPPNKKSKFFQNCNDLDLDL
                     VFRLHNECCASKTTLSLRGNIKVPRIVSLPVRENERKSHYESKLVGESLISNGKVAVL
                     IVAGGQGVRLGHNRPKGTFPVSPIMKKTLFQLLSEQVRALSFRYNAKIPLLIMTSVEN
                     HDDTVLFFDTHNFFGLDQNSVYFFQQGMLPTITPEGKLLLKDETSLFVNPDGHGGSLS
                     ALNKSGLLDMLMEQGFSELFYCQIDNPLVRIADPVFLGYHSQANSQCSTKVVRRQRID
                     EKVGVYVSLDGKDAILEYSDFGGKHMEALDDNGEILYWAGNTAIHIMNLSFVKTLNTH
                     GFALPYHCANKNVEILDSKGTRKNVDAWKFETFVFDAIPFAERTCCMEVDRSEEFSPV
                     KNKSGPDSPSTAHESMVKLHKRWLHDAGILVPHERLVEISPLFSLDREELISKLKGTI
                     MQVESDLYLA"
     gene            complement(25023..27113)
                     /gene="recG"
                     /locus_tag="PK125_03405"
     CDS             complement(25023..27113)
                     /gene="recG"
                     /locus_tag="PK125_03405"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF008168.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HPB37187.1"
                     /translation="MEKENDITKKQIRYIKGVGPKIAALLNKKGLNTVEDLFYFLPNR
                     YVDKRSIKNINEITEGETALIIAKVTSSRSVYFPKARRRAFEATAQDSTGSLTLRWFN
                     VVLPYLKELCIKDNTLLLSGRVTKFGKNLQMVHPEAVLLASEAKREEHQGIIPVYPEI
                     SGLKQGTLRNIINQAFLQYADSLCSIIPSGLEDLLGVPRFKDMLIKIHFPDETIVDEA
                     SRQKYIDRLIFEEYLLFQISLQIKKQSTKNKKGIAFINKGDYRMFFERGLPFKLTNAQ
                     RRVIEEIEKDMGSESPMNRLLQGDVGSGKTICAIAAACIAIGSGYQVAFMAPTEILAE
                     QQYFAIHRFFDDMGLTVTYLRGNMGKERMDIIDSIKRGSTNVVVGTHALLQDDIEFKS
                     LGLVVIDEQHRFGVMQRKILKEKGLFPDTLVMTATPIPRTLSMVVYGDLDVSIIDEMP
                     VGRQKIMTRVFFNRENHIAFQQVEEELKNGGQAFIVYPLVEESQKIDLLNATNMAELL
                     QKTVFPNHIVGLLHGRMKPDEKERVMLLFKKKQIDILVCTTVIEVGIDIPNATIMVIE
                     HAERFGLSQLHQLRGRIGRGSKSSKCFLIASEKLTPVARRRLKVMEKTTDGFQISEED
                     MRLRGPGEMFGLKQSGIPEFRLGNLVRDGYIMSRAKKAAEQVLPKLSNRELDQIKERA
                     DMKWEDPVSVADIA"
     gene            27567..28112
                     /gene="pyrE"
                     /locus_tag="PK125_03410"
     CDS             27567..28112
                     /gene="pyrE"
                     /locus_tag="PK125_03410"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR00336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HPB37188.1"
                     /translation="MVEKDKLLMILKDLSYEEGDFTLVSGKKSTYYIDAKETTLNPEG
                     MFLIGKLMYQMAKEIPGIEAVGGVSIGGDPLVCAVVLESYNKKDNLAGFLIRKEPKGH
                     GTNRWVEGGKNLKKGMSVVILEDVITTGGSSLRAIEVTEKEGYNVKGIVAILDRLEGG
                     KEAIESKGYLFRSIFTLKDLR"
     gene            complement(28133..28744)
                     /gene="yihA"
                     /locus_tag="PK125_03415"
     CDS             complement(28133..28744)
                     /gene="yihA"
                     /locus_tag="PK125_03415"
                     /inference="COORDINATES: protein motif:HMM:TIGR03598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HPB37189.1"
                     /translation="MKILTAEYLTTVVKPEKREFRGIPEICFIGRSNVGKSSLINRLA
                     MRKIARTSSTPGATKAINLYRVGYEFRGLRKWTIFSDFPGFGYSKVARSTYLGWESMI
                     DTYITENSSIQGFAWAFDIRRDPDILDKTVLEWIEYSRLPFCLILTKCDKERRSYGLS
                     KRDTLKKEFCTDDVFVFSSKNGYGRDEVLTHLFSTLSKATESV"
     gene            complement(28741..>29389)
                     /locus_tag="PK125_03420"
     CDS             complement(28741..>29389)
                     /locus_tag="PK125_03420"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: protein motif:HMM:TIGR00758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HPB37190.1"
                     /translation="FVMKFPVTDRTVRGFLLALKGMGIESYVVNKKDTMTLSALQRAV
                     RGCRRCNLASTRRNVVFGEGDNRARVVFVGEAPGEEEDIQGKPFVGRAGKLLDQLIEQ
                     AGQRRESVYICNILKCRPPNNRDPEGSEIALCKNYLLTQIDLIKPKIICTLGRHAYNT
                     LMNVDERITRVRGILKDYRGMILLPTYHPSFLLRNQDKIKEALEDMERLKQLLKV"
BASE COUNT         8442 a         6647 c         5683 g         8617 t
ORIGIN      
        1 aacccagtta tcgagaagct ctatcttgcc tggtattaca ccattctcaa gaatcctgaa
       61 gacattctca cctgctttgg ccacatcatc atacacgccc atgattgtct ttcttgcctg
      121 aggcaggggg ttaagcttca gttcagcctt tataataaca cccagggtac cttcagagct
      181 gatgaaaata tgcaacaggt cgtaaccaac aacattttta agggttctgc ctccaaggtt
      241 cacaatctcg ccagtaggta gtacaacttc aaccccaagg atatactgtt ttgttactcc
      301 gtatttgaca catgcagggc cgcctgcgtt ttcagctatg attcccccca tggttgcgcc
      361 aagaaagctc tggggatcag gtggaaagaa gaggccttcc ttcccaagcc ggatggtcag
      421 atcctgaagc accacgccag gttccacagt agcagtaagg ttttctttat cgatctttaa
      481 aatcttgttc atctttgtgg tacatagaat gatgcctccc ataatgggaa ttgagccgcc
      541 acttacgttt gtaccgccac ccctcggtgt aacaggtatc ttctccttgt ttgcgagctt
      601 catgatttcc gaaatctctt tagtagtggt aggaaaaaca actacatcgg gctcgtgttc
      661 ccagcttgtt gtcccgtcgt aagaataagc cttgagcgtc tccttctctg ttttgacgtt
      721 ctctttaccg acgatttttt gcagttctgc aatgactgat tctttaatca ttcatgcccc
      781 ctttgattct gaatattttg aacaatttct ctaattttta tattgttccg cataatttgt
      841 caagaaaata cttgtataca aaccaacaga atgtgcaagt tttcacagct ttgcttttta
      901 taagtaattt cgattaatct gtattattat aacagtgcat tacgccaaac aaaaaatagc
      961 aaaagtaaat gaattcaagg cccataatgg ttataacagt tttataatgg caatcattga
     1021 aacaattgta ccaatcttcc ttatcatcat ctttggtttt atactccagc agagagggct
     1081 gttaaaacgt gaatttatcc aggaggccaa cagatttgtc tttcatttca gccttcctgt
     1141 gctcattttc atcggaatca tcaagtcaga tattagagag gtaagtttaa ccagtattct
     1201 gtccgtcata gtcccaaccc ttgttatact ttgtattgct tttctgcttg ccctggcaat
     1261 gggtttaaaa aaagggactc tcgggagttt tgttcaaacg acttttcacg ggaatatcac
     1321 gtatattggt ctggctgtcc tctactatat gatgggtgaa gaaggtatga ggagaggaag
     1381 catactcata ggattcctaa tactcatgaa caatacacta gctattgcaa tactttcctg
     1441 ggcttctcgc agacacgaaa acatatggaa atcgctcgca tctgttgtta aaagcccttt
     1501 gattatcggt acagtcctcg gcatgtttct tctttatttg gatatccctg ttcccatatt
     1561 attgatgaaa accatgggta ttcttgcaaa tattgcactc cccatggccc tcattattat
     1621 tggtgcttcc atgtcaatga acactatcag gaactcattc aagctgtcag ggattgttgt
     1681 cttcatgaag ctcatggttc ttccttccct ttcttttgcc ttttgccatc tgtatgcaat
     1741 tcccttcact gacgcccttc cagggattat tcttctcgcc acccccacag ccactacatc
     1801 attcatactt gccaaccagc ttggtggtga tactgatctc gcatcgggtg tcattaccct
     1861 ttccacgctc ttatcaccct ttgtgtttat cttctgggct tatgctgcac ggtgatttgc
     1921 caatctcagg caaactcttc tgcagaacaa aaaaatccat tcctatttat tcctaattta
     1981 ttcaaactcc catccagcat aaccatcttc gccctatgtc cggatacttc tgctacagct
     2041 ttaaacttga ggtcccccta accttacatg gccaccattc gagcaagcca cctttaccga
     2101 tccatttcgt aacttcaagc tctgtaagca ttcctcaaga gccaatcaga ttgattatgg
     2161 catcctgttc gttgctcatc tattttctgt taaatgtatg ccgggacttt ggcagcttag
     2221 gttaggtcct ttaaaactat attcattttt ttaaggttgc ttttattatc aataaggttc
     2281 ctgcctgcta tcttgtcaag cacgtagatc aaattaccac cattaagagc atatttctgt
     2341 ccataggaaa tagggacttc agggttcaca tctccaagga gtgaccgggt aacagactca
     2401 acttttcttt cgccgtttgc taagagcact acatttcttg cctgatacac aagctctgca
     2461 ctcattgata tggcataatt tgggtaatct ttttttgaga tgaagtgtcc atcagttacg
     2521 gaatctttaa tagtgttatc atcaagcttg acaagcataa ctgaacttcc ctcaaaaggc
     2581 acacctgatt catgaaaagc cacgtgcccc cgccccccga cgccaattat ctgaatatct
     2641 atgcctccgg ctttttttat tttctgcatg tatccgttta atatctcctt tcttatccat
     2701 gaaaggtatt ctgatcccgt tttttgtttg ataacgatcg attttcctgc acccgtgcct
     2761 ttacaagccc aatcatgggg atatctcttc agctctttta ttagtacttt ctggtcgatg
     2821 agcgaaccaa aaggcacatt tgtttcaata aatttcttct taagaagccc gaaaaactcc
     2881 tggatcatga aatagcagta gctttcagga tgcagggccc gctgctgggc attttctccg
     2941 ggcagtccaa tgtattcatc aaggttaaag cttcgtatcc tgctgctgtc gaattcccca
     3001 ttgtttgccg ctctcgccag atgtttgtac atccctgtcg gggagttccc tgttgcaagc
     3061 cccaagacaa cttctttctt ccttttcaac aattcaacca gttttttctt gactatttcc
     3121 gctgcgacct tgctgagatg ttcaaaatca ttcgttatga ctacctcaat aggcatacta
     3181 ctcctccagg cctagattta tgagccaaac tgatgagaat ttctcaaccc agcacattct
     3241 ttacaacatc agcaatttct tcacagtatt tctcagtaac actatctgaa ggcccctcaa
     3301 ccatgaccct acacatgttc tgggtacctg aatacctcac aagaacacgt ccctcgtctt
     3361 tcaattctct ctcgaccttg tctattattt tcatgacttt aggcaccgta tttatatcgg
     3421 gcttactttt cacgtccacg ttgatcagct tctgaggata tatatccatc attttggcaa
     3481 gttccgataa aggcttgcct gtcttgacca tggccgcaag tagttgcatt gctgtaacaa
     3541 ttccgtctcc tgttgtatga tgatcgagaa ataccatatg tcctgactgc tccccaccga
     3601 ttatgctgcc cagcctgatc atatcctcga gtacgtatct atccccaact ttagaggcgt
     3661 gatatttaaa accatatctc ttgcaggcaa cccttagccc aaggttgctc attactgtgg
     3721 caacaagcag gttgtttttc agtttaccct gatctttgag catctttgca cagatcagca
     3781 ggatttgatc accagttatc tcctggccct tttcatcaac cgctatgagg cgatcaccgt
     3841 caccatcgaa ggcaagcccg attgcagcac ctttttcaac gacccttttt ctcaggtctt
     3901 ctgtgtgctg cgacccacac ttgtcgttta tgttgatgcc atttggggag ttgtgaatga
     3961 catctatatt cgcaccaagt tcccagaagg tatccggagc aaccttatac gtagcaccat
     4021 ttgcagtatc caggacaatc ttcatccctt ctatggataa atctcgagga aaggtgtttt
     4081 ttaaaaacac aatgtatcga ccattcacat cctccatacg aaacgcctgt cccatatcct
     4141 tcacaggcgg cacgagtgtg tgaagtgtat tgctcagcat cagatcctca atcgtttctt
     4201 cctgttcatc cgataacttg aaaccactac cagaaaatat ttttataccg ttgtcctggt
     4261 agggattatg ggaggctgaa atcacaatcc ctgctgcagc gcgcatactc tgtgctataa
     4321 aagcaatacc tggtgttggc aatactgaaa gaagataggg gtagccgccc attgaagtta
     4381 ttcctgattc aagcgagctt tcgagcatgt agcctgagat acgggtgtct ttcccaatca
     4441 ttattcttgg ccgtccatgt tccttcttca aaatgtagac aacagcctgc ccgagcgcaa
     4501 aggctatttc agcgttcatg ggatagcggt tagcttctcc tctgacgccg tcggtaccaa
     4561 aaagtctacc catctataac tcctccttac aatttgaaga ataattagca acctaccagc
     4621 ttaaaagaag gcacaattgc cttcacgcgt tcacaaaaaa tatcgacaat cttttgaagc
     4681 cacaatacac cttttttaga gacatgagca ggcaatccct gtataacagc tatatatttt
     4741 gcctcttttt ttcctcttaa cctttcaaac tcgaaaaaaa agtcatcata atacctttcc
     4801 acggaatcat cacatattcc ctctgtaaac agtctctcta tatcagaaaa tctttcgtgg
     4861 aattcattcc ttcctactgt atctgaccct tcttcgctct cgtttttaag ggttattttt
     4921 gacttttctg ctaacacctg caatcgcttt atccgctctt ttttcgcaag agcaagtttt
     4981 tcgagcaacc cagaaaaaat ttgctgtccg aactcctgtg attccaaaaa tgaatgcctg
     5041 acatgaatat accactgttc aagagccatc aggttcgcaa tgtatataat attgttaccc
     5101 actatccgag aaagaccttg gtatgctctg ggcttgaaat tcactgtctt tccagaatat
     5161 gactttccca ctattagttt attgtcttcc ggaaaatcat ttctcagcac agaattggca
     5221 gccaccacat taccataccc catccgtagt ggccctacaa taccaccctg ccctccgaga
     5281 aagatgggag gttgatttag catgacgcct cttggaacat caccaatcag tgatgctgtg
     5341 cttttgtctc catccggcgt gaaattaaaa tgaatataag aacttccaac ctcactgtga
     5401 tttttacggc tttttcctcc ggccattaaa cagtcacaga aattgatcag actaccaagc
     5461 gtcacaaaag ggaagagtat ggtctgtttt aaaccgacac agtgagcacc atttgcctcc
     5521 tcttcaagta tgcaaccttc acgcacctgg gctcccaaac ccatgcttgc cttttcgaga
     5581 aatacagatt tattaaaata tcctcctttt agctcaacca tggatccaag ctgacaatcg
     5641 tctattgtga caggtccttc ataaccaatc tggcaaccat tcgatatgac agtcttttca
     5701 ccataaatcc tgcatccggg gtaaattttg acaccttggc ctgatatatg attaacatct
     5761 acctcatggc ctacatcaat ggagaaagga tttggaatat caaccccttt atcgatcatc
     5821 tggacgattt tatggtgaca ccgcaatttt atatccatgg catactcccc ttcccgatct
     5881 gctacatatc taaatttatc aatatattat cgaacattgt gaccactaat ttagtggttt
     5941 cgaaacgtgc actgtcattt ccatttagca atcaattcaa acaaatcaaa cccatcgtga
     6001 gaccttatgc taccattgta ccttgtgtga cagataaccc gtatctaaac tgtttttaac
     6061 atccattgag aaaaccatac tgaaccactt aaagacggcg tacattaaca cttttttgtt
     6121 tttatgtcaa taaaaacaag gtcttgctat caaacaattt gacaagcatg aaccaacctt
     6181 gctatatgtt taagggtgac gtagatgaaa gaagtcctcc gaaagctgct tcggacacta
     6241 agccatagcc taccggagat cccctttgaa atacgctatt gggataatca atcagaacgg
     6301 ttcggaacag gagaaccatc cttcacaatc atcttcaagc atcagaaatc gatcaaaaaa
     6361 atattttcaa aaggttctat gggctttgct gaagaatacg tagaggggaa catcgatgta
     6421 gagggagatt ttgtatacct ggtccgtctc ggcatatgcc ctgaatttca gaatataaag
     6481 ctgtcttttg gcacaaggat ggcagccctc atgaaccatc ttttgtccct caacaccctc
     6541 aggggctcag ccagaaacat tgcccaccat tatgatctag gtaatgaatt ttacaggcta
     6601 tatcttgatg agagcatgac ttactcttgc gcatactata gaaacaagaa tgattctctt
     6661 gaagatgccc aacaacaaaa gtacgaacat atctgccgaa aactagccct taaggaaggc
     6721 gagactctag tggacatagg atgtggttgg ggcggtatgc tcctgtatgc agtccatcat
     6781 tacggggtta aaggattggg ttgcacactg tctgtgcatc aagctgatta cgcgaaaaaa
     6841 cgcgttgccg aggaaggact ggaaaagagc ataaccatcc ttttggagga ttaccgtaat
     6901 gttaagggtc aattcgacaa atatgtatcc attggcatgt tcgagcatgt aggcaagggc
     6961 ttaatcccta tgtttatgga gaaaactaag tccatattga aacctggtgg aatcggtcta
     7021 ctccatacca taggaaaaga acgtgacacc ccttttgatc cctggactat gaaatatatc
     7081 tttcctggcg gccacctacc agccctggat cacataattc gaaccatggg cgataaaagc
     7141 cttatcccgg tcgatatcga aaacctgcgc ctccactatt cggcaacact tgatgagtgg
     7201 gccaaacgtt ttgaaaaaaa ctcggaccag atcaaaaaaa tgtttgatga gagacttgtc
     7261 agaacctggc gcatgtacct ccgtggcagc tctgctgcct ttcgatgggg tgatataagg
     7321 ctttaccaga ttctttttac caatggactg aacaattctc ttcccatgac gagggaacat
     7381 ctttataagt cctagattcc aaaaatgttg gtttaaacgc atctgcatgt catttaggct
     7441 ctgaaaccag tgctgtattt cccatcactg tctattgaca atcagccttt tttctcagat
     7501 aatgagtgca catattctgt atctgaatat cagaacagca agaggatcat gtgcataaca
     7561 tattaaaatc aaacaaactg gaccatgtat gttacgaaat ccgtggcccc ctgctcagag
     7621 aggcgaagcg tctcgaagaa gaaggatata ccattatcaa actgaacagt ggtaatcccc
     7681 ctgctttcgg tataaatgcg ccagacgaga taattcgcga tgtgattctc aatatcaggt
     7741 cagcccaatc atacggcgat gcaaaagggc ttttcgctgc acgaaaagcc atcatgcagc
     7801 aatgccaact cacaggcatt gaagatgtgg agattgaaga tatttacgtt ggcaatggag
     7861 taagcgagat gatcatgatg gccatgcagg gactcctcaa caatggggat gagatgttgg
     7921 tcccttctcc agattaccca ctatggaccg cagccatcaa tctctccgga gggaaggctg
     7981 tccattacat cactgacgag tcttctgact ggaatcctga cattaaggat atggaatcaa
     8041 aaattactgc aaaaacaaaa gggatagttg tgattaatcc caacaacccc actggtgctg
     8101 tatatcacag agacatttta atcaagatta tcaggctggc agagaagcat aatcttataa
     8161 tatatgcaga tgagatttat gatcagattc tctatgatgg cgccaaacac atcccattgg
     8221 cgtcactctc gaaggatgtc ctctttgtta ccttcaatgg actatcaaag tcacatcgca
     8281 taccaggtct cagagccgga tggatgataa tcagcggtaa aaaacaggta gcatacgatt
     8341 ataccactga aggattggac acactttcca acatgcgcat gtgtggaaat atggctgcac
     8401 aattcgccat acaaacggca ctgggtggcc gtcagagtat tattgacctg atttcccctg
     8461 gcggaagatt gtatgaacag cgaaaggtgg cctatgaatc ttttaaggcc atacccggca
     8521 tctcatgcgt caaaccaatg ggagcactct atctttttcc aaagattgac gttaagaaat
     8581 ataaaatcta tgatgaccag aagtttcttc tagattttct attggaaaaa aaggtgctca
     8641 tggttcaggg aacaggattc aattggaaaa aaccagacca cttccgcgtt gtttttctgc
     8701 ctaatgttga agaaatcaga actgttgcca tgcacatggc tgacttcctg aaagaatacc
     8761 gtcagatata aatctacctt ctcgattctt attgatggca ctttgattct ttatggacct
     8821 ggtttgcaat tcttttttca tctctttgct accagatccc actcaatatc aggatctcag
     8881 attgtcggtc ccatgctgca ctgtcaagtt agcatacaag cgcatgaaca gcaactcgtt
     8941 gttgtacaat actttggtca gtttcttagc tgatcattga tctgagtttc tctagcactg
     9001 aagggatgcc attcttttcc atttcatagc tctctataat gaccttgcct gaatatcctt
     9061 cttccctcag aaaattaagc atccttttaa tatccatatc tccgtcggga aacagatgat
     9121 cgtctcgttt tcccttatta ttatgaagat ggacatgggt tatgttgttg attcccaggt
     9181 atttaagatc actgatgatc gcctcttcag gttttttctc ttttttagca atctttgtga
     9241 gaaaagcatg acctgtgtcc aaggtcatgc caagatccgg aaacacatcc ttcagctctt
     9301 taaattctgg cagatcatag aaaaagaaga gatgcatggg gattgactca agggccaagg
     9361 taacgtcctt cttccttgca taggataata ttgatctcat gtcgttgata aaaaacttcc
     9421 aggatttctc agcaacatcc tttatttttc ccatccagaa tgtatatcca ggatggagaa
     9481 caacagggtt ggcaccaata gcataggcca tgtcgatcgc catctttact ctctttctcg
     9541 aaaattggcg aatctcaggc tgatggcttc caaggtttac ctcaaagaac ggtgcatgaa
     9601 tggaaaattc taatccatca ccagccagtg cagccatctg ttcaacaaag tccttatcca
     9661 ttttttggac atggggtgtc tcgagtgaca gttcaattgt ccttacgcca gaggagataa
     9721 tgtcatcaat aacctcattg attgactttt gtatgaagaa aaaagtagaa aacccaaatt
     9781 tcatgtgata ttgtgcaatc agcaatgagt gttcagcaat cagccttttt atgttgttaa
     9841 gctaattgct gaacactaaa cgctaatgtt tgaactatta acactttgct atactgtaac
     9901 catcacttaa ctgcttcctt aatctggctt acaatctcat cgctgctcag ggaagctatt
     9961 gtttttaaaa gtccttcctt tgcataaaaa tcaataaggg gcgctgtctt ttcattatat
    10021 gtttcgagac gttttgtaat agcctcaaca gtttcatctg cacgttgtac tgcaggtgaa
    10081 ccgcacttga gacacttgcc atcaggtgac ggtggcttgc ttttaatatt gtatatttcc
    10141 tggcaatccg ggttagaaca cgtccttctg gtggtcaacc tatcaaggat aacttctttt
    10201 ggaacatcga tgttgatgac cgcatcaagc ttcatattaa gtttcgtgag gagtttcttg
    10261 agttcttcag cctgtggaat ggtccttgga aaaccatcaa gaagatagcc ctttgcacaa
    10321 tcaggctctt gaagcctcgc ttccataatg tccattataa gcctgtcagg aacaagttcg
    10381 cccctctcca tatatccctg tgcctcaagt ccaagtttag tctttgcttt gacagcattc
    10441 ctcaggatgt ctcccgtgga aatctgtaca gatccatcaa aatcactcag caatttcgca
    10501 atagttccct ttccggcgcc aggcgctcct agtaatacaa ttctcattac aattcctcct
    10561 ttaatcagat attgctactt attctttttc tcttcctttg cttttctttt ttctttcaag
    10621 gttttttggg gagccttttt agcttctttt ttctgcttac ttttctcagc cataaatata
    10681 cctcccgtca cgatatttat tgagtctatc aaagtaaggg agaaaaatca cgcaaaaaca
    10741 atcgatgcat tagggaacag gataaccctg taatccgttt tatcctttaa tcctgattta
    10801 ctctttcaat cgttctctgg ttcttcaact gttggcaacg aagtgtgcca ttgcatgaaa
    10861 aaccatctat cgtcacgttt ttccagcact gctgtaagtc gtgcaggcat gaccatctcc
    10921 atccccctcg cctctgcatg gatttgaata tccgttgcaa gccatgccac taacccagcc
    10981 gaagacaccg aactccattg cacctcaagg gatatggatg catcttgacc ccagtcatgt
    11041 tgggcctgtt cacgaatacc actgatacct atactcttct cgtctttgcc gtttccataa
    11101 aatacaacat caggatcaga ggcaaacaaa ttcaggatac catccatgtc tttatcattg
    11161 taagccctga tgaatctacc caggacatcc ataacatctg cttctgtttc actatctgct
    11221 ttcatattaa cacctcaatg agaaaggatc atggggtttc aattcttctt ctcagccagt
    11281 acgttagctc gttttcctca ttaagtatct gtgtatgaca caggaacttg caaatgatac
    11341 agccattgat accacagttc ctccgataaa tcccagggtt atatatgtgt atcttaccat
    11401 tattccataa ataacaacac ttatcgctcc cagtatagat gatttcatga ccgcagccga
    11461 aaatgtggtg ccatgagaaa aatatgtcat gaaaatcatc cccacaaaca ttgctggaaa
    11521 cattacaaac atacctccaa tgagcggacc ccctattttg gtgactatta tcgcagaaac
    11581 gatgaccaat ccacccagaa cacccctgaa aatcattatg gatagtgtat actgcatctg
    11641 ccttcctgcc ttcgacctta cctttagcac cttctcaaca atgtagtgag tgattaaaac
    11701 tagacaggcg tagctgattg ctgaaaccgg gaaactattg aaattaatga gaactatccc
    11761 aaaagaaagt agtgtccaaa taaggaaagc ccctccaagg gcaacccaga agttgatctt
    11821 taaaagaaag atatacgcaa caatgaagat gcaatttatc cctccaatga ttggaacaat
    11881 agttgttgca tcaacagcaa cacgttcaga ttgcgtccag gcgataaaga aaagcgagat
    11941 caggatggtc gatggcaaac ctgccatcaa accacctatt cttgtgccgt atttctctgc
    12001 gaggatagtg accatggtta tccatagact tcccacaata aatgaaagaa tcagcttaag
    12061 tacaaaaata ttatccatag atcgttatat atttatatga tgtagcatta ctgtcagatt
    12121 aaataaagtt ctctgcaaca tcttatttct cacatcataa agatactcag gattcagggt
    12181 aagaatgtcc atcaggcttt tttagttaaa aatattcacg aataacatgc tctaggcatt
    12241 acaatgaagg gctgtatttc gtctggcata tgatgtagat caaaagctgt agtagcaaat
    12301 aggcacagtc gctatcacat acctcccctg gtgccagcat gagaacagcc tgcgctgcca
    12361 ctttaatata ttgggcagta ggagcaagta tggtaaacat aacaaagggc ccacctcatt
    12421 cgggaatcaa tgagtcaccc atcataaacc gttgttttac ctggaggccg agcttggatt
    12481 tagattattt tggacagcag tataatacca taaaaaaaac agatcacatt atccagtctt
    12541 ccataccctg caactcgaca ctattcaagc tctttgagat gaaatatgag tgtactggcc
    12601 agaccaacga atataccgag aattgtgatg cccagcctaa aacttaccat atcaccggat
    12661 attcccagca aataaggaag cacaccccct ccaaaggcta tgcttactga taggataatt
    12721 cctgttgcca gacttctcat ttctcggtta aaggtcttgg caagtgcaac gaggcctaca
    12781 gggaaaaaag ccgttacaac aaatgcctgt agaaacagga caaatcctat gtatttcaca
    12841 gatgacaagc ccataagaac agtgaatacg cttgtagtga tcattacgaa aaacatgatt
    12901 cttcgaagat taaacctatc aatgagaaaa ccgcatgcga ttgcgacaaa aatggctccc
    12961 agccttgata tccccaatat tgtgtttgca aaccctatat tcatctttag ttccttcgtc
    13021 aggtacaggg gcaccacgga atagattcca aggtttgccc ctgcaccaaa aatccaaatt
    13081 actgccatga accagaggga ttttattttc ataatgctgg taaatacagc ctttgggggg
    13141 tcgttcactt taatttccga gcttgtcatt aagaagacaa cagagcatat cacgtaaaca
    13201 catgcaagaa ctatgaatat gccacgccag ggaaaaaaat gaagcaggaa aagcgctata
    13261 aaaggggtgg caaagattga gcatgaggca cctgtatcat gaatagcaat tgctttaccc
    13321 caatcttttt ccgcgtaata ctcggtgatt agcggaatta cagctggtaa atagaagcct
    13381 acggaaaaac cgagcataaa tgcaaaaaaa taaaggatac gaaactcatg aactaatgga
    13441 attaagaaag ccaccagact caagagtaaa agtgagaaca ctatcgatct tttatatccg
    13501 aacctgccgg aaaaaagtcc tgacactatc actgcagcac catatccaac ggatagaaaa
    13561 acaaatatac tgcttgcttt tgcatggctg accaaaaact catcttccac aagcggcagt
    13621 ataggcgcaa aggctatcct cgtggtaaag tttgtaaacc agatgaacca caaaaaaaac
    13681 aaaaaaagga gggccttgcc ttgtaaatta tgataccgct gcattctttc aatctaacct
    13741 aacacccttt aagatttcca caaaactatt catgatgact acttttgttg tacaatatac
    13801 gattaaagat tctctttacg tttactcttg ctagtaaaaa gggggttgat aatgaagaat
    13861 gatccagccc gatgggtgac aaaagtgatt gagaagttta atacaacatc tgataatagc
    13921 ctgatgaatc cacaaaatga acctgcatgg gacaaaccac tcgttggatt tgcaaacgga
    13981 aacgatccat tgtgggacga atacaaacga catatcggtg atttttattg gacacctcat
    14041 gagatatttg aactgacatt tccttctcta tctgtaaaac cagacgaatt aacagttata
    14101 agctggattc ttcctcagac caaacagacc aggttggacc aacgaaaaga aaacagcctt
    14161 ccttcagaac ggtgggcacg gtcacgaaaa tatggtgaag aatataacat gaagcttcgt
    14221 cttcatcttg taaaatcctt caacgatgaa ggtatcaaag catgcgcacc cctgctttct
    14281 cctctttgga aacagatggt gtcaacaagc tgtgggcttg catcaacatg gtctgaacgt
    14341 cacgcagcat ataccgcagg cctcggcacc tttggattgt gcgatggttt gataacccct
    14401 ctcgggaaag cagttcgttg tggttctgta atcgcacgga tagccatcaa agcgactgaa
    14461 agaccctatg aggatcccca cgcttattgc ctctatcact ctgaaggaac atgtggaaag
    14521 tgtatcctca gatgtcctgc cgatgctatt acaagagaag gacacgataa gaacaaatgc
    14581 caacagtatg ttcatggtgt ggcacatgaa tttatcaaga gcaagttcgg ttttgatgct
    14641 tatggctgcg gattatgcca ggttgaggtc ccctgcgaat caggaattcc aagcaaaaag
    14701 gggatagaca atggatgaat gactatacga aaacataaaa aaatccgaaa gaaacaaaac
    14761 tctctctcag cttaattgtg atcgcgttat ggctaacgtt tcacgggcca tatcctgcgc
    14821 tgctaatgta aaaaaccatt cttttattcg attttcaaga taattaatat aggaaatccg
    14881 ttcgatacca aaacggaaac tatctatatt tacacctgta gtagtgaaac gccacaattt
    14941 atcagcatcc ttaacgattt tgtcatttag tgaaagagct tctaaacgtg tatcatgacc
    15001 atcaatgata tccattattt catgtatctt ggatccagga taattgagct cattcaaaat
    15061 ctggcctgcc atttttgctc cctcaatttc atgaaacctc tgtacatcat tttttttgac
    15121 ttccggaccg aacgcccctg cctgttcatc ttctggcacc attttccatc ccacatcatg
    15181 aaggagaatg gcaggcaaaa caatgtcttc atctgcctct gggtaatgtt ttagtaatct
    15241 ctttgtaaat tcataggaaa gctccacatg gacgtcatta taacgtgtat taagatacgg
    15301 ctttgccatt tcgtaaattg tttcgtacat tgttcgcatt ctgttcttta tcctggtata
    15361 tggcctcttg tgtcgatttt caaatttaat cttttttacc cttcctacct ttcctgcttt
    15421 cctctctttt tggccttact tctcaatttt catcatcttc cctgccttta tatacttcat
    15481 gcccatccag gttcctaccc ctgcaaatga aatcttcaaa taaatatcag gcagttgatt
    15541 ggctgtcaga gcacctaggt aaccacctac aagcgctccc atggccagac cccatgcaat
    15601 atcaaggttt acattaccaa gcttgtaatg cgttgaagct ccactcatgc tggcaggaat
    15661 catagcgagt agcgaggtac cttgtgccat atgttgtcct acgtttccca caacgaccat
    15721 aagcggcacc atgactcctc ctcccccaac ccccatcatt cctgacacaa aacctgttac
    15781 agatccaatg gcaagaaaaa taaccatgta tgtccactgc ccgggggtcc aggttgttat
    15841 tggaagataa cctttaacaa caagcaagat tgacgcaaaa atgagaaaac aaccaaatgc
    15901 cactttgagt tttttttctg gaagagaatg ggcatagaga gctccaaagc gcgctgttat
    15961 tgtggcgctc acagcaagga tcagggctat cttcaagtca actcccccaa ggccataata
    16021 ggtaacagca cccacaatac ctgtgaatac tatggcaaca aggctggtac catgggcctg
    16081 gtgttgggta acttttgcaa gccacgtcat gagaggtatc attataactc caccgcccag
    16141 tccaagaaga cctccgaaaa agcccccaag agttccactt gcaagaccta tggtaaactt
    16201 tgtactgcga gtcaaacaat gcaccttttc gttactgtgc ccgaatatat cattttaata
    16261 atcatttcgc catcggtaaa atctgtgatc cgtaacgtgt aatcggtaat ctccgttgta
    16321 ttccatgacg tgttacaggc caccatcacg gatttctcaa atatcagtca aacatgctat
    16381 actatgacca tgaaaaagat aatatccttt gatcttgatg gaacactggt ccacggaaaa
    16441 tatggtaata tggtctggaa ccatggcata ccccaagaat attcccgtaa gtatggaatg
    16501 ccattcaaag aagctgagca gcttatcagg caccagtatg aggaggttgg tgaggccaac
    16561 ataaagtggt accatataga gtattggctc aagaggtttg acctgccagt ttcggcaaaa
    16621 acgcttcttg accgctttga gtcatatatt gaagtcttcc ccgatacaaa ggaggtcctt
    16681 gaagccctga aaaacacata taccctgatt gttgcttcaa acgctgcaca aatttttgtc
    16741 caaaaagagc tctcctatgc aaatcttaca tcctatttta cccacatcat ttctgcaaca
    16801 tccgactatt gcatggttaa aaaggaaata aggttctacg aaaagctttg tgatacctta
    16861 agcatatctc cttatgagat tgtccatgtg ggagatcatc atatttttga cttcgagacc
    16921 ccatcacaac tcggtatcga cgcctatcac tttgtacctg atcataagga tagtagcaac
    16981 caatcacata cagacaacgg aaagaccata cattgtttga gtgacctgct gaatatatta
    17041 tgaaaacatg ttacaagtgc cagcatcccg taatgttgga gtttatctca cggcgggacg
    17101 agtgccaagt atgcgggtca gatctccacg tctgtttaaa ctgctctttt tttgatgaaa
    17161 cgaaatcgaa caagtgcaga gaaccccagg cagaatatgt aaaagaaaaa gaccgctcaa
    17221 attactgtga cttttttcgg ttcagagatg atgtacagaa gaaatcaggc aaggaagaag
    17281 caaaaaaatt gtgggatgaa ctgtttaaga aggtatgaag tgctttacat ttaatacatt
    17341 ctactaagat acacaactac acccaaatga caaacccaca cacatgcagg aaatatcaag
    17401 gcactcttcg ccgacagctg ggttgtatcc aatcacctgt tagtttgcat tatccttaag
    17461 aagccctatg acggtactgc gaatggcggg gaaaactccg acggcttgca accaaagcac
    17521 atgcgtaata gatggctgcg gtgcaagtcc cagttataga catggtattg agataaaatg
    17581 aaatgaaata tcttttcgca attatccagc agtactcgct attgcgctat atcctctgtt
    17641 ttctgcacgg tgatttttag ctttcttgga gggaacgggc caaaatattc ataaagataa
    17701 cacttgatgt ctccattata gagtttcctg tttttgtcgg attttttgcc aaaaaatgat
    17761 tcaaagccag gatgggcgtt gaggataaag aatgaccatg ttttgagttt ggaaaaaact
    17821 tcccccattg atcgaaacag ttgttcaacc tcttttgaat tacctatacg ctgaccataa
    17881 ggaggattgc atatgataca gccatatttc ttatgtgagc tgaactgttc tacaggaagt
    17941 cgttgaaatg taataacctc acctacacct gccctcacag cattgtctct tgctttcttc
    18001 agtacccgtc catccttatc agatgcaagt attcggaacc ttgtggtatt ctctttcatt
    18061 cgtgcttctg atcttaccgt gtcccacatt atttttgaca tattctgcca gctttcagat
    18121 acaaaatttc tctttaatcc aggggcaatg ttccgtccaa taagggctgc ttcaataggg
    18181 attgttccag aaccacacac cgggtccaca aggactcgct ctggtttcca tctgcttaaa
    18241 aggacaatag ctgcggcaag attctctctc aaaggcgcct caccggactc ctcgcgatat
    18301 cccctcttgt gaagcccctg ccctgtcgta tcaatggtca ggattgccac atctttcacc
    18361 agggaaattt caatcctata aagcggccct gtctcaggaa accgggccaa atggtatttc
    18421 cggttcatgg ctttgatgac cgacttctta acgattgtct ggcaatctct gacactcgcc
    18481 agttttgatc gtacagattt gcctgttaca tgcatttttc cgttcacggg aatgatatct
    18541 tcccaagcaa cattgaacgt tccctgaaaa agttcatcaa agtctgttgc cttgaattct
    18601 gccattttta tcaacactct gtctgctgtt ctaagccaga tattacaccg tgcaatatct
    18661 ggctctttgc ccttaaatat gactctgccg ttttctacta taaggtcctc atatccgaga
    18721 ttccttaatt cctgtgctac aacagactcc aatccgaaac ttgaggtagc gatcagcgcg
    18781 taatttgcca tcagtatttt atctttctta aaaacagacc ccttgccggt gctgttggtc
    18841 ctgcattctc ccggtctttc gaatttaaaa tcttgatgaa ttcttctact gttatcttcc
    18901 ctgatcctat aagtacaaga gtccctgtta tgtttcttac catgtatctg agaaaccctt
    18961 tcgcttcagt cacgaaatat atgagatttc ctctcctttt tacatttgcc ctgaatacct
    19021 ccctcacatt gcttttatag acctcgtctt tctttttgaa cgcagaaaaa tcatgctctc
    19081 caacaatgac tttcgctgca tcatccatag ctgatgtatt taattggtaa tgaatatgcc
    19141 atacatagct attgagaaat ggtgaattat aaggggtgtt cagtatgcaa tatacatacg
    19201 tttttgaacg agccgaatat cttgcatgaa atgtgatgtc ttcttctcga gcatgtttca
    19261 ctcttatatc cctgggaagt atgctgttta aaccctgtat aaggcttctt aattcgattg
    19321 tcctttctgt atggaagttt atgacctgtc ccatggcatg aacaccagcg tctgtcctcc
    19381 cgccagcata gattgtagtt gcatgattga gaattttctt gagggaattg ttaaaaattt
    19441 cctgaattgt gagaacatct ggttgacact gccagccgtg atagtttgtc ccgtcgtacg
    19501 aaattgtaag tacgatattt ctcataaaat cctgattcca gcgactggtt ccttatgtga
    19561 atgccaagaa aggtattgga tgacgtgaat cgagcaacct ggatttcctc ggttatcagt
    19621 tctctacttt gccggctttt tatcttccgg gaggttcttt gtcaccccat gatcttcaag
    19681 cctcttatag agttcctcaa cctccttttt taaccccaca ttctcagctt taagaaatgc
    19741 attttcttcc ttcagcattt tgatctcatc tttaaggcct ttcgtttcct gaccacagga
    19801 gttgaggatt gtccccataa gtagaaaaat tattaataat gatacatatt tcatcactat
    19861 ttatttgcca tgatgtctca ctcattgtca acagcttctt gtttgtggta cattaaatga
    19921 aatcgaaacg aattccaact gagacatcta ttttggagga ttgaatgcac gcatctgttg
    19981 ttacggtacc catagaagaa gaaatgaaac ggtcatacct ggattatgca atgagcataa
    20041 tcgtaggccg ggcattgcca gatattcgtg acggattaaa acctgttcac cggagaatcc
    20101 tttatgcaat gtacgaacta aacaacaccc atgacaagcc atataagaaa tctgcaagag
    20161 ttgtcggtga tgtcataggt aaatttcacc cccatggtga tcaggctgtt tatgacgcaa
    20221 ttacccgcat ggttcaggag ttctctctcc gttacccgct tattgacggt cagggcaatt
    20281 ttggttctat tgacggcgat tctccggcag ctatgcgata cactgaggtg agactctcac
    20341 ggatagccga agaaatacta aaagatattg aaaaagagac tgtattgttc agggcaaact
    20401 acgatgaatc attaatggag cctgtcgttt taccctcgag aataccgaat cttctcatta
    20461 atggttctgc aggcattgca gttggtatgg caaccaatat cccacctcat aaccttacag
    20521 agattataaa tggcatttca gcctatatcg acaatcctgg tgtaacactg gatgaactta
    20581 tggagcacat accagggcct gattttccaa cccagggtat aatttacgga cgccagggca
    20641 tccgggaggc ctatgaaacc ggcagaggcc atattgttct tcgagccaga gcaaacgtag
    20701 aagtgcgcaa aaaagatggc agagaagcga ttgttatcac tgagatccct tatcaggtca
    20761 ataaagcaaa gctcatagaa agcattgtag agctcgtaaa tacaaaaaga attgaaggca
    20821 tctcaaatat aaaagatgaa tcaaacagag agggtattcg ggtagtcata gaattgaagc
    20881 gaggggaaat ggccattccc atattaaaca agctatatag acacacccag cttcagacca
    20941 cctttggcat aatttttctc accatagtga acaacgagcc aagggttctc aacataaaaa
    21001 gcatcataga agaatttgta aatttcagaa aagaagttgt cacaaaacga tcaacctttg
    21061 agctgaataa agcaaaggac aggcttcata tcttggaagg tttgaagaag gcactggagc
    21121 atattgatga ggtcattgcc cttatcaaaa aggctcccaa tactcaggag gcacgggcat
    21181 cattgatggc tcggttcgat ttttcagaaa agcaggcgac gagtatcctt gaaatgcgcc
    21241 ttcagaggct cacttccctg gaaagaaata aaatcattga tgacttcaat actacttctg
    21301 cggagattgc cagactggaa cagatactcg cagatgaaaa attacttctt gcacttgtta
    21361 aagaggaatt gctcgagata caagacaagt atggtaacga caggcttact gatattgtag
    21421 atgcattgcc ggatattacc atcgaggaca tgataaaaga agaagaggtc gctgttactg
    21481 ttacctataa agggtatatc aagcgtacct cactcgatca gtataaaaat cagttacgtg
    21541 gaggaaaagg aaaaatcgga attgttgtta gagaagaaga cattgtagat cacctgtata
    21601 ttgcctctac ccattcccat cttttctttt tcacaaattc cggcaaggct atatcgatta
    21661 aggtgcatgt catcccagaa gctcccttaa cgagtaaagg aagagctgtt gttaatctga
    21721 taaatataga caaggatgag cgcataactg caatatgcca tgtcaaagaa ttttcagagg
    21781 ataaatatct attcttcgct acaaaaaaag gtcttgtgaa gagagtaaac ctcagtctct
    21841 ttaaacacct gcgatcgtcc ggcataaggg caataagcct tccggaagat gacagtctca
    21901 ttggagtcct cgagactgac ggccatgcag aggttattct tgcaactaga aaaggtatgt
    21961 caatcaggtt taaagaggaa gaagtccgac ccatgggaag agtagcttat ggagtcaggg
    22021 gtataaaact gaagaaaaat gatgaggttg tctctgtcga aaatgtagaa cagggatgca
    22081 atgtgttcac tgttactgaa aaaggctacg gaaagcgtgc tacatgcacc ggctacagga
    22141 ctcaagcaag gggcggcagc ggagtaatca atgtcaggtg cggttctaaa aatggacagg
    22201 ttgtttgcct gaaaaaggtt acagaaaatg acgaggttat ccttatcacc gacactggca
    22261 ggacaatacg ctttgcaacc aaggatattc ctcttcaaaa caggggtggc ctcggagtca
    22321 aactgatgga tttgcagaat gaaaaaacga ttaccggtgt agcgatcatc ggggaggatg
    22381 aaggctcgga aacagagaaa tcagaaaata atcatggata aggctatagt ccccctttcg
    22441 tggacgttta cctatgtgac aaaaggtagg gtctgatgaa aataggcatc attggcgcag
    22501 gagcttgggg tacagccttt gctattcatc ttgcgagaca aggacaccag attcttttat
    22561 gggtgtatga accggaactc tttgatgttc ttcaaaagac cagagaaaat actttctacc
    22621 tcccttcatt tcttctcaac agtcagattg atcttaccct gagcctgaag gacttcgcca
    22681 atttctctga cgacgttgtt gttgcaatac cttcatttgc cttgagaaaa accctcgaac
    22741 cagtatctcc tttattgtct ggcaaacgta ttcttgtcct tacaaaaggg tttgaggaaa
    22801 ataccttcat gcgaatgagc actgtgatac acgagataac tcaaagcagg gtgcctgttg
    22861 catcactgtc cggtccctct tttgcgaaag aggtagctca cggctttttc acatcggtag
    22921 ttatagcgtc aggcgggaac gaagtttcaa aatattttca gaacattgtc cataaccgga
    22981 attttagagt ttatactagc gaagacatca tcggtctcga ggtcgggggt gcgatgaaga
    23041 atgtaatggc tattggcgct ggaataatcg atggtttaaa acttggaaca aacgcacaag
    23101 cagcattcgt gacacgaagc ttagccgaga tcaagcgcct cgggaaagct atgggcgcca
    23161 aagaaacgac gtttatgggc ctttcaggaa taggagatct tatccttaca tgctatggtg
    23221 gactcagtag aaatcgggcc ttcggcatag aactgtctaa aggaggtaaa cctgaagaaa
    23281 tcattgtgaa ccaaaaacag gttgtggaag gttattatac tattaaagct tctcacagcc
    23341 tttcaaaaaa actcgacgtt gatatgccca ttaccagtga actgtacaag attgtctttg
    23401 aagccaagaa tcttcaggat tcaattgaag atatcacgtc ccggggattc aaggatgaag
    23461 atgcctaacc attctttaca cttcccattt tttgttgctg acgtgataaa acaggaggta
    23521 ccccaaaagc acagaactta aagcgaaagt tgattttttt atgaaccttg cgataatctt
    23581 gtataattca gatcaagttt aggatcaata aacaaaagag gattccatgg ataatcgtgc
    23641 aattcttgac ctgcttcaag taaagaatca ggatcatatt attcatcacg ttgacagatt
    23701 gcctccgaat aagaaatcaa aatttttcca gaattgtaat gatctcgacc ttgatctcgt
    23761 tttccgactt cacaatgaat gctgcgcatc caaaaccaca ttgtcgcttc gtggaaatat
    23821 caaggttccc cgaatagtct cgttaccggt aagggaaaat gaaagaaaat ctcactatga
    23881 gtctaagctc gttggtgagt ctctcatctc caacggaaaa gtagctgtgc tgatcgttgc
    23941 agggggacag ggcgtcagac ttggccataa caggccgaaa ggcacattcc ctgtttctcc
    24001 aatcatgaaa aaaaccctgt tccagcttct atcagagcag gtgagagcgc tttcattccg
    24061 ttataatgcc aaaataccac ttcttataat gacaagtgtc gagaaccatg acgatacagt
    24121 tctgtttttt gatacacata atttttttgg cctcgatcaa aacagcgttt attttttcca
    24181 acagggtatg ctgccaacca ttacccctga agggaagctt ttattaaagg atgagacaag
    24241 tcttttcgtc aatcctgacg gtcacggtgg gtccctcagt gcgctcaaca aatcaggtct
    24301 ccttgatatg ctcatggagc agggtttttc agagcttttt tactgtcaga tcgacaatcc
    24361 gcttgtaaga atagctgatc ctgtatttct gggataccac tcccaggcca attcgcagtg
    24421 ttctaccaag gttgtcagga gacaaaggat tgacgaaaag gttggtgtct atgtatccct
    24481 tgatggaaaa gacgcgatcc tcgagtacag tgatttcggt gggaaacata tggaggccct
    24541 tgacgataat ggagaaatcc tatactgggc gggtaataca gccattcaca tcatgaacct
    24601 ctcttttgta aagacgctta acacacacgg ctttgcccta ccctatcact gcgctaataa
    24661 aaatgttgaa attcttgatt caaagggaac ccgcaaaaat gttgatgcct ggaagtttga
    24721 aacatttgtc ttcgatgcaa ttccttttgc tgaaagaacg tgttgcatgg aggttgaccg
    24781 ctctgaggag ttttcgccgg taaaaaataa atcgggtcct gactcaccat caaccgctca
    24841 cgaatcaatg gtaaaacttc ataaacgatg gctgcatgat gcaggaatat tagttcctca
    24901 tgaaaggctg gtagaaatca gcccactctt ttctcttgac cgggaagaac tcataagcaa
    24961 gctaaaggga acaattatgc aggtagaaag tgatctatat cttgcgtaat taccagttct
    25021 tgtcatgcta tatcagcaac actcaccggg tcttcccatt tcatatcagc acgttctttt
    25081 atctgatcaa gttccctgtt cgacagtttt ggaagaactt gctcggcagc tttcttcgcc
    25141 ctgctcatga tgtatccatc cctgacaaga ttacccaatc taaactcagg gattccagat
    25201 tgttttaaac cgaacatctc tcctgggcct ctcaatcgca tatcctcctc tgaaatttga
    25261 aatccatctg ttgttttctc cattactttg agtcttctcc tggcaactgg tgtcagtttt
    25321 tctgaggcta tgagaaaaca ttttgatgac ttggagcctc tccctattct tccccgaagc
    25381 tggtgaagct gcgacaaccc aaacctctct gcatgttcaa taaccatgat cgttgcattc
    25441 ggaatgtcta tccctacttc aattactgtt gtacatacca gtatatcaat ctgttttttt
    25501 ttaaaaagta acattaccct ttctttttcg tctggtttca tccttccatg aagaagaccg
    25561 actatgtggt tggggaaaac tgttttctgc agcagttcag ccatgtttgt ggcattcagg
    25621 agatctattt tctgggattc ttcaacaagc ggataaacga taaatgcctg ccccccattc
    25681 ttcaactcct cctctacctg ctgaaatgca atgtggtttt ccctgttaaa aaagaccctt
    25741 gtcattatct tctgtcgccc tacaggcatt tcatcaataa tcgaaacatc aagatcacca
    25801 taaacaacca tggaaagagt cctggggata ggagtagctg tcatcacaag cgtgtcagga
    25861 aacagacctt tctcttttaa tatttttctc tgcatcacgc cgaatctgtg ttgttcgtca
    25921 ataaccacca gaccaagact tttaaattca atatcatcct gcaaaagcgc atgggttccc
    25981 acaaccacgt tggtgctacc tcttttaata gaatcaataa tgtccatgcg ttctttaccc
    26041 atattgcctc gcaggtatgt aacagtaagt cccatatcat caaaaaacct atgtattgca
    26101 aaatactgct gctccgcgag tatttctgtg ggtgccatga aagcaacctg atatccgctg
    26161 ccaatggcga tacatgcagc cgctatggca catattgttt ttcctgatcc tacatcccct
    26221 tgaagtagcc tgttcatagg agattcgctt cccatatctt tctctatttc ttcaattacc
    26281 ctgcgctgtg cgttggtaag tttaaatggc agcccccttt caaagaacat tctgtaatca
    26341 cccttgttga taaaggctat gcccttcttg ttttttgtgg attgtttctt tatctgcaat
    26401 gaaatctgga aaagcagata ctcttcgaag attaaccgat ctatgtattt ctgacgtgat
    26461 gcttcgtcaa ctatcgtctc atccggaaag tgaatcttaa tcagcatatc tttgaaccgt
    26521 ggaactccta ataaatcttc tagtcctgaa ggtataatgc tgcacaaaga atctgcatat
    26581 tgaagaaaag cctggttgat tatatttctc aacgttccct gcttcaaacc ggatatttcc
    26641 ggatatacag gaatgattcc ctgatgttct tctcgtttag cctcactcgc aagaagaacg
    26701 gcttcaggat gaaccatctg cagattcttt ccaaacttcg taaccctacc tgatagcaag
    26761 agggtgttgt ctttgatgca aagctctttt aaatacggga gtaccacatt gaaccatctg
    26821 agagtcagtg accctgtact gtcctgagca gttgcttcaa aagcccgtct tcttgccttt
    26881 ggaaaataaa cagagcgcga gcttgtcacc tttgcaatta tcagcgcagt ttcaccttcg
    26941 gttatctcgt ttatattttt tatgcttctt ttatccacat acctgtttgg tagaaaatag
    27001 aaaaggtctt caacagtatt caggcctttt ttattgagca gtgcagcaat ctttggacca
    27061 acacctttta tatatcttat ctgttttttt gttatgtcat tttccttttc catggagact
    27121 ttttatatta caatattata attggaaaga taatcactaa cagcgcatta attacttttt
    27181 gctcttttta caggaaaact tggatcatgc aaaaaatatt aagaaaacca gagataatat
    27241 ttcgaactgt acggtgttta aggatttttt aactcaaagg aagcatcaaa cataatatgt
    27301 ttctgccttc acatcttaca ttactgatcc agaaaagtgc cggtacagtt gagtgcccat
    27361 ggggtggcag ttgcaattga aacctatatg aacttttctt ttgattgcct gttttgtgat
    27421 gcctgtttct ctggcagttg tttgctaaag cttcaatgtg atatgtggcc acacatggta
    27481 aagcacatgt aaaaagcctg gtatactggt ggagagccta aatcgataaa gaatttaaaa
    27541 tattttacaa gggagattga tcaactatgg tggagaaaga caagctttta atgatcctca
    27601 aggatttatc gtatgaagaa ggcgatttta cccttgtgag cggcaaaaaa agcacctatt
    27661 acatcgatgc caaagaaacc acgctaaatc ctgaggggat gtttctcatt ggcaaattaa
    27721 tgtaccaaat ggcaaaagaa ataccaggta ttgaggcagt tggcggagtc agcataggtg
    27781 gagatccgct cgtttgcgct gtagtactgg aatcgtataa taaaaaagat aatcttgcag
    27841 gatttcttat cagaaaagaa cctaagggcc atggtaccaa cagatgggtc gagggtggta
    27901 aaaaccttaa gaaaggaatg agtgttgtta ttcttgaaga tgtcatcaca actggaggtt
    27961 cttccctgag ggcaatagag gtaaccgaga aagaaggata caacgttaaa gggatagttg
    28021 ccatccttga tcggttagaa ggcggtaaag aagcaatcga atcaaaagga tatctattta
    28081 ggtcaatatt cacattgaag gatttacgat aaatatattg atttaataaa ttttatacgg
    28141 attctgttgc ctttgatagg gttgaaaaca agtgggtaag tacttcatcc ctaccatatc
    28201 cattctttga tgaaaatacg aaaacatcat ctgtgcaaaa ctctttcttt agcgtatccc
    28261 ttttactcaa accgtaactg cgtctctcct tgtcacattt ggtaagaata agacaaaatg
    28321 gaagccgact gtattctatc cattcaagaa ctgtcttatc gaggatgtca ggatctctcc
    28381 ttatgtcaaa agcccaagca aacccttgta ttgatgaatt ttctgttata taggtgtcaa
    28441 tcattgactc ccagccaaga taggtggatc gtgcaacctt cgagtagcca aaaccgggga
    28501 aatctgaaaa aatggtccat tttctcaatc ccctgaactc ataacctacc ctgtaaagat
    28561 taatcgcctt tgttgcgccc ggggttgagc ttgttctcgc aatttttctc atagcaagcc
    28621 tgttgatgag ggacgatttt cccacgttcg atcgtccgat aaaacagatt tcaggtatac
    28681 ccctgaattc tctcttttca ggcttaacga ctgttgtcag atattcagct gtcagtattt
    28741 tcataccttg aggagttgct tcaaacgttc catatcttct aatgcttctt taattttgtc
    28801 ctgattgcga agaagaaaag aaggatgata agtgggcagg agaatcattc ccctgtaatc
    28861 ctttaagata ccgcgcactc tggtgattct ctcgtcaaca ttcatcaggg tattataggc
    28921 atgtcttcca agagtacaga ttattttcgg ttttatgagg tcaatttgag ttaacagata
    28981 attcttacaa agagcaattt cagatccttc aggatctcta ttattaggtg gcctgcattt
    29041 caggatgttg caaatatata cgctttcacg tctttgacct gcctgttcaa ttaactggtc
    29101 aaggagtttc cctgccctgc caacaaaagg ctttccctgg atatcttcct cctctcctgg
    29161 ggcttcacca acaaatacaa cccttgctct attatcccct tcgccaaaga ctacattccg
    29221 tcttgtcgaa gccaggttgc atcttcggca gcccctgaca gccctctgta atgctgaaag
    29281 ggtcattgta tcttttttgt ttaccacata cgattcaatt cccattccct tgagtgctaa
    29341 cagaaaacct ctgacagttc gatctgtaac tggaaatttc ataacaaat
//
LOCUS       DAOOMC010000020        29035 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdus sp. isolate AMR_MDS_2685
            NODE_1414_length_29035_cov_5.715631, whole genome shotgun sequence.
ACCESSION   DAOOMC010000020 DAOOMC010000000
VERSION     DAOOMC010000020.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864760
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdus sp. (wastewater metagenome)
  ORGANISM  Syntrophorhabdus sp.
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae; Syntrophorhabdus.
REFERENCE   1  (bases 1 to 29035)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 29035)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,729
            Genes (coding)                    :: 2,708
            CDSs (with protein)               :: 2,708
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 7 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29035
                     /organism="Syntrophorhabdus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1414_length_29035_cov_5.715631"
                     /isolate="AMR_MDS_2685"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2699756"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(107..709)
                     /locus_tag="PK125_03425"
     CDS             complement(107..709)
                     /locus_tag="PK125_03425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB37191.1"
                     /translation="MEALKLTFQQTKRRLWKSLLIYGLTGIIMLMTLAGAIAMKEYVD
                     SLYATLDKLQEFNIKYIKVRTAIDDIEKSLVRVKAMLPHDSSGLSDKEHMLIALDDLK
                     AKAGSSEITVANIEDKGADLQLSVVIRSVLKDYTSFVNFAGYLQSLKFPFFGITGIRI
                     QRSDDQVTSATSFELKGILKFPKATFDTQAEPTKKIPGKL"
     gene            complement(697..2055)
                     /locus_tag="PK125_03430"
     CDS             complement(697..2055)
                     /locus_tag="PK125_03430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB37192.1"
                     /translation="MSKQIAVSFESGYAKVSYGSQRNGTLVITRTFTVKEDEFDAFLA
                     REKTKDFIVLFSFQTFYQDVLLLPRVEMKYVDTLIEAEIKKNAPELKNFVFFSTILKE
                     KVHEGRPVRETFVFAVDSTEINPILERFSRFGKRVKRLYADVFVLSCLVVGLDNVKDK
                     TAMCMIDKGDYKTLFLVREGKLTFVRSIQSRGRGIDRYDVTNVNMTVNYARQTLREHP
                     EELIIVNTAKDEVSTVEGLTLPTTIFRYPPGVMATGGDITGFVIPVAGLFLGKGHVRE
                     SLLPKLYQRVVSQEHFMRYASVAFVIVSLLCLGYIISGWNQVRQIRGMIDPIKAEIGS
                     KRRVYEELESRNKDLQAVLPYINYLNAESAKPDIQKALATLQILNRDKVRVEEIEIIN
                     PGEELTIRVKGHITANTYGDLQSCFRKLADDMKSAKSGQVLIEKLDLTSKDFNIETTW
                     KH"
     gene            2186..3232
                     /locus_tag="PK125_03435"
     CDS             2186..3232
                     /locus_tag="PK125_03435"
                     /inference="COORDINATES: protein motif:HMM:NF017183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative Ig domain-containing protein"
                     /protein_id="HPB37193.1"
                     /translation="MKNYFGERGLTLIEMAIVLVIIGLLVSVGAALIGPLTKRAKLTE
                     TREIVKNAFESILGFAIANKRLPNQLSDLGIKTSDAYLNNLIYYKAYADTPPSYNLCT
                     SVGTYVTVNDQGVGKTNVAFLVFSAGENRVNNTGTASPFSIQIPGVGGYDDVALYMDI
                     GTLRQKACNVLRIVTDSLPTATEETVYPTSALEATDGTLPYTWTITSGALPPGLTLIP
                     GTGVIAGTPINDGSYKFTIQVTDSDNPQRTASKSLAITVNPDKPRITTEFLTYGTVGQ
                     PYPSSTLSASGGAAPYSWTLAGGSNLPPGLSLSSGGIISGRPASPGTFAFTVTVIDGR
                     SRMATKNLSIAVYP"
     gene            complement(3462..3695)
                     /locus_tag="PK125_03440"
     CDS             complement(3462..3695)
                     /locus_tag="PK125_03440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB37194.1"
                     /translation="MNRDEQLVAEGWERRFIACEPRLSEMVEMYKEIGFEVHLEPLPS
                     KDEIDQASCEESGCTACYDAGPERYRIIFTRKT"
     gene            complement(3731..4735)
                     /locus_tag="PK125_03445"
     CDS             complement(3731..4735)
                     /locus_tag="PK125_03445"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HPB37195.1"
                     /translation="MYKFRSYKTPQRILVTGGAGFIGSHLCERLISDGHEVLCLDNFF
                     TGTKQNILHLLPNPRFELITHDVTQPILLEVDWIFNLACPASPIHYQYNPVKTTKVNV
                     LGALNMLGLAKRVRARILQASTSEVYGDPEVHPQKEEYWGSVNPIGKRSCYDEGKRVA
                     ETLFFDYLRQNSVDIKVIRIFNTYGPRMRPDDGRVISNFIIQALHNKPITIYGDGLQT
                     RSFCYIDDLIEGMIRMMDFETGSHKKSRDYTKPHLSGMPGPINIGNPHEVTIKDIAQK
                     VVTLSQSKSAIVYEDLPEDDPKRRCPDIAKAKQLLDWTPKISLDEGLEMTIEYFRNNR
                     "
     gene            complement(4728..6056)
                     /locus_tag="PK125_03450"
     CDS             complement(4728..6056)
                     /locus_tag="PK125_03450"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="HPB37196.1"
                     /translation="MRISVIGVGYVGLVSGSCFAETGNDVMCMDVDSEKINKLREGII
                     PIYEPQLEELVKNNIKEGRLRFTTDIKEAVDHGLILIIAVNTPQDHDGSADLKYVMKV
                     AHDIGKAMEKYRIVVVKSTVPVGTCDMVKAAIQEELEKAGRNVSFDIASNPEFLKEGV
                     AVDDCMKPERVIIGVEHDRVEEILRELYAPFTRTGAPFIVMDVRSSEMTKYAANSMLA
                     TRISFMNQMANICDRLGADIMMVMRGLGSDSRIGPKFLFPGVGFGGSCFPKDVRALVK
                     TSRDLGYNPSILESVMDVNENQKVSFTEAVKDFYKNDIKGKKFAVWGLAFKPNTDDMR
                     EAPSQYIVKTLTGQGALCHVFDPKAMDSAKPLFSGNQNVQFGKNQYEVLEGADGLILV
                     TEWLSFREPDFERMKGLMKEPVIFDGRNQYNPKTMARHGFRYICIGRPRV"
     gene            6422..6886
                     /gene="rimP"
                     /locus_tag="PK125_03455"
     CDS             6422..6886
                     /gene="rimP"
                     /locus_tag="PK125_03455"
                     /inference="COORDINATES: protein motif:HMM:NF014617.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000028 - ribosomal small subunit assembly
                     [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HPB37197.1"
                     /translation="MIPQREIIEQLQDILLPVLQDYALELVEIEFKPSGKRWLLRLYI
                     DKEGGVTIADCENVSREFGRILDVEDIIDHPYSLEVSSPGLTRPLKKWEDFQRNRGKL
                     CKIVTREKIDDKNEFHGTIMDASEDTIEIKGTNNVFTIPIYAIKKANLEFEF"
     gene            6896..8230
                     /gene="nusA"
                     /locus_tag="PK125_03460"
     CDS             6896..8230
                     /gene="nusA"
                     /locus_tag="PK125_03460"
                     /inference="COORDINATES: protein motif:HMM:TIGR01953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HPB37198.1"
                     /translation="MYFDLNYIIEQVGKEKGIPKETLIEALEEAILIASKKKFGNHLD
                     LEARYNEELGEIEVFQFKTVVEEVTENDMEISLTDAKNHDPECQVGDSIGIKMDTSSL
                     GRIAAQTAKQVIVQKVRNAESDVIYNEYKDKIGEISTGVVQRIEKNHYIINLGKTEAI
                     LPMKEIIPGEAFRQRDRVKAYILDIEKTQKGCTIHMSRTHPNFLAKLFEQEVPEIQEG
                     IIKIIGAAREPGERAKISVYSEDPDVDPIGACVGVKGSRVQTVVQELRGEKIDIIPFS
                     KDPAKFVCNTLAPARVSKVYLNEEEHAMEIIVNDDQLSLAIGKKGQNVRLASRLTGWK
                     IDINSESELELTSRKVIDELIENLKISEILARILYDEYLREPHDIAKLTPEEMNKITS
                     ISIDDCVRIIEQARLIRDIESREKEAEAVTEKENIVVPALKDDEATDQASTQ"
     gene            8266..10854
                     /gene="infB"
                     /locus_tag="PK125_03465"
     CDS             8266..10854
                     /gene="infB"
                     /locus_tag="PK125_03465"
                     /inference="COORDINATES: protein motif:HMM:TIGR00487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HPB37199.1"
                     /translation="MAKVKITTLTDKISDDEILTRLKGLGVKIKDKAKEEPTLAKKDD
                     EKLTSSGETMVEKRVASTIIRRRVKPPQPQKEKAEVVEEEVKPEEKKPEKKETVVRRA
                     SRKAEKVEEKKEIVQPQDQPEIIKIEKIEKKEEHVAPVLTERDEDEEQNEEALKGDIE
                     IIGEEKKKEITKVLEEAYKQDLEKDFTLVESEPDEGIEEKKKRKTERLLKKIEEQDAE
                     ETKLKKKGILKRKVVIKEEDLYTFRRQKARPIPGRKDRKEKPEEKKIEEKVAELKPTK
                     KIVKVGEEIQIGELAKRMNVKAQDIIKKLLPLGIIATVNQDIDFDTAYLVGTELGFEI
                     EKIISIEEEFFAREEEGKENEKNLHPRPPVVTIMGHVDHGKTLLLDTIRHSNIAEREA
                     GGITQHIGAYVVDVNGKEIVFVDTPGHEAFTAMRARGARVTDIVVLVVAADDGVMPQT
                     IEAINHAKAANVPIIVAINKIDRQNANIDKVLKELAEQGLVPEEWGGTTLLAKISAKK
                     KIGITELLELIILQAEMLELKANPDMRASGIIIESELDKGQGPVGTVIIQEGTLKIQD
                     PFIAGTMFGRVRAMIDDKGKRIQKAPPSTPVLVVGFQDVPHGGDRFIATTEERYAKEL
                     SRYRQEKLRERETAKSSRTTLEELYSKMGKSEKIQLGVIIKADVRGTIDAITEALKKL
                     SNDLVEIQIIHGGAGAITETDVNLAMASGAIIIGFNVKPIGKAQALAEQEKIEIRTYS
                     IIYDMIDDVKKAMEGLLEPKLVETDIGKAEVRKVFAVSKIGTVAGSYILEGKVTRNAM
                     ARVMRNSNHLFTGKISSLKRFKDDVKEVQAGYECGISLEGFNDLQEGDSLEFFVQEKE
                     RQTLDG"
     gene            10860..11159
                     /locus_tag="PK125_03470"
     CDS             10860..11159
                     /locus_tag="PK125_03470"
                     /inference="COORDINATES: protein motif:HMM:NF016351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF503 domain-containing protein"
                     /protein_id="HPB37200.1"
                     /translation="MIVGISTIEIFFPENQSLKDKRQALKKIVEKTRTKFSISIMEIN
                     QNNLWQRAQIGFSVVGVKRDHVYRMIDNVHEYVESLYIGTIIDTKTEIIVMGNEI"
     gene            11149..11499
                     /gene="rbfA"
                     /locus_tag="PK125_03475"
     CDS             11149..11499
                     /gene="rbfA"
                     /locus_tag="PK125_03475"
                     /inference="COORDINATES: protein motif:HMM:NF014130.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HPB37201.1"
                     /translation="MRYRKLRLQDQLREEISFIIQREIKDPRVGFVTILEVKMSNDLK
                     IAKVYCSVYGEDEDKKKTIEALQRSKGYIKFRLGKRIQLKYMPDLTFLIDNTFEKAAR
                     IEEILRKEVYVQED"
     gene            11483..12433
                     /locus_tag="PK125_03480"
     CDS             11483..12433
                     /locus_tag="PK125_03480"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF013528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HPB37202.1"
                     /translation="MFRKIKQIIDGGNKFLITTHIDPDGDAIGSCFSLYWALDGLQKN
                     PFVYMRDRVPYMYEFLPKPTQLFYNEPPDGTYDGIFILDCGNFFRVGNGHERLKSQGP
                     IINIDHHATSEKFGIINIINQNSSSTGEILYNIYDALMIPISYNIAVNLYTAIFTDTG
                     SFRYPNTNSEAFLICEKMTKFGVSPAHVAQMVYENHPKERFVLLGEVLSSLETYENDT
                     IALAVVTKEMFHKTNTAKEYTEGFVEFIKEIRGVNVAILMKELSDNQYKISMRSKGDT
                     DVALICNHFGGGGHRNAAGCTINGSIIQIKDRLKEALTKP"
     gene            12430..13314
                     /gene="truB"
                     /locus_tag="PK125_03485"
     CDS             12430..13314
                     /gene="truB"
                     /locus_tag="PK125_03485"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HPB37203.1"
                     /translation="MNGFLVIDKNTGMTSYDVIRRLKKLHPFNKIGYIGTLDRNATGI
                     LPVAFNNGVKLIPFLENSEKTYRATFLLGVTTDTLDIEGKVLSSVDVKEFKRDILARE
                     LNKFKGKIVQQIPAYSSKKVNRKPLYKLARKGIAVEPHLKEVEIFSIKFLDYNHPYLD
                     VEVTCSKGTYIRALSQEFGANLGCGSTLYSLKRTKHGEFTEDMAVNIEHFKIEEDVLH
                     YAMSLEAILKTLRETVVETALEKFLKHGMPIPLSGDTKEWQNGEYTKLLNKKGNLIGI
                     GMADTFSKTIKIKRLINI"
     gene            13325..13594
                     /gene="rpsO"
                     /locus_tag="PK125_03490"
     CDS             13325..13594
                     /gene="rpsO"
                     /locus_tag="PK125_03490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011275926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HPB37204.1"
                     /translation="MALTPTQKKNIIDGYRIHEKDTGSPEVQIALLTERIKSLTEHFK
                     KFGKDHNSRRGLLVLVGSRRRLLTYLKEKNVDRYKKIIERLGLRK"
     gene            13623..15710
                     /gene="pnp"
                     /locus_tag="PK125_03495"
     CDS             13623..15710
                     /gene="pnp"
                     /locus_tag="PK125_03495"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009567342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HPB37205.1"
                     /translation="MEEQITIDYAGRQLTISTGALAKQADGSVVVRYGDAVVLVTVVV
                     DKRASAKDFLPLTVNYHEMSYAAGKFPGGFFKREGRPSEREILMSRLIDRPLRPLFPK
                     GFTNEVQVIATVLSADQENDPAIMGILGASCALTISEIPFDGPLAGVKVGRKNGSFVI
                     NPTPLDLEESELDVVVTGNKEAIIMVEGQAKFVNCEDLIEAIHFGHQKLIPLIEIQEQ
                     LKEKVGRQKWEVIVDSRLDALVQELKGKAEEDILKAFSASGKQERSKKQNDVFDGLIS
                     VYPDIEEALMKTAFEEITRDILRKQLFATSRRIDGRTWEDIRPLSCQAGILPRTHGSA
                     LFTRGETQALALTTFGTSEDEQKVESLMEGETFKTFMLHYNFTPFSVGEVAMLRGPTR
                     REIGHGNLAERALTPILPPKEDFPYTIRIVSEILESNGSSSMATVCSGCLSLMDAGVP
                     IKEPVAGIAMGLVKEGDNEIILSDILGDEDHLGDMDFKIAGTRDAITAIQMDIKIKGI
                     SKETMQKAVVQGQKGIRTILDAMAQTLEKPRETMSPYAPRIFTIRIKPDKIRDVIGPG
                     GKIIRGIVEQTGVKIDIEDDGMVKIASIDEGSANQAIDIIKKLTKEAELGEIYTGKIK
                     KIIDSGVIVEILPGVEGYVHVSQLAEGYVKKATDVVKEREEITVKVIEIDPTNGRIKL
                     SRKAALKEQKAQE"
     gene            15727..16986
                     /locus_tag="PK125_03500"
     CDS             15727..16986
                     /locus_tag="PK125_03500"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HPB37206.1"
                     /translation="MYRKTILENGLTVVTESIPYFSTVSLGMWWKTGGRFENEANNGI
                     SHFIEHMLFKGTLKRSAYDIAKEIDAVGGAINAFTGKEYTCLYARVLRKDTDIALDIL
                     SDMYKNPLFQEDDLEKEKYVIAQEIKMIEDSPEEYIYEMFNASYFKGHSLGLPILGKQ
                     ENVEKFTRHELRDHFHRHYALYNLVITASGRIDHDVFVQKVSKFFNCNQSHGPTPETM
                     AVPSPCAGFHIYEKDLEHVYLCIGTDGVSQVDNRRYRLYLLNAIMGGSMSSHLFQEVR
                     EKRGLVYNIYSYVNCYHDAGTFGISTSTSHESVEEVLTLIKQEMIRIRDTGITDAELS
                     FSKEHLKGNLFISLEGSETRMGRLAKNEIYFGNYIPLKDTLREIDKITKYEVDQIGNS
                     IFKNPENISLTILGNVNREQIEVLWKG"
     gene            16974..17414
                     /gene="dut"
                     /locus_tag="PK125_03505"
     CDS             16974..17414
                     /gene="dut"
                     /locus_tag="PK125_03505"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: protein motif:HMM:TIGR00576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HPB37207.1"
                     /translation="MERLEVVIKKKDGAILPSYGTEASSGLDLAAFLDKPVVLAPFAR
                     ALIPTGIYIAIPEGFEAEIRPRSGIAHKYGVTVLNTPGTIDSDYRGEVNVLLINLGDQ
                     PFTVNNGDRVAQIIFKTIVTIDWRVTEELPDTKRGHGGFGSTGK"
     gene            17563..18432
                     /gene="xerD"
                     /locus_tag="PK125_03510"
     CDS             17563..18432
                     /gene="xerD"
                     /locus_tag="PK125_03510"
                     /inference="COORDINATES: protein motif:HMM:NF001399.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HPB37208.1"
                     /translation="MNLYYALDNFITYLTAERGASPHTVDAYNRDIISFIRFLEEKGL
                     SLSERTERSFIEIYIAHMRELGKKTRTIVRAISALRGLFNFLLADGIITHNPLEDIEI
                     PKYSPPVPEVLSEQEVLELLQLPNGSKTSLRDRMILELLYATGLRVSELIKLKKSDIN
                     LDGGFVIASGKRSKERVVPLNIYSKNTIKAYLEEEKPQGAYLLPNKRGGMLTRQAIWK
                     IIRKYATKMARDNVSPHTMRHTFATHLLEGGADLRSVQILLGHEDISTTQIYTHVDSK
                     RLREIHKKHHPRG"
     gene            18432..21071
                     /locus_tag="PK125_03515"
     CDS             18432..21071
                     /locus_tag="PK125_03515"
                     /inference="COORDINATES: protein motif:HMM:NF013528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HPB37209.1"
                     /translation="MKVITSHHNADFDSLSSMVAAKKLYPDALLVFPGSQEKTLRDFL
                     IHSTLHVYDIAKIKNINYDEVDTLILVDTRQKSRIGDLAKVVDKDSVKIHIYDHHPPS
                     SDDIKGDLEIIKNTGATATIIIHILKDRGIPISPEEATIMMLGLYEETGSFQYPSTTT
                     QDFDAASFLLSKGANVNLVADMLVKELTPEQVFMLNDLISNATVYSINGVDIVITEGS
                     TEKYVGDLAVVVHKFRDMENINVVFALFRMEDRIYIIARSRIPEVDVGHILSLLGGGG
                     HKEAGSSTIKDMTIIEAQEKLLKYLRHNVKPLWKAKDIMFFPVKSIDARCPISEAKSI
                     MVKYNINALPVLNEDRIVGIISRQIVEKAAFHKLENLPVQEYMSTDASTVKSDDSIER
                     AKEIIIGGNQRFLPVTKDGKLVGAITRTDLLRVLEEEISKSVRGKMEFQETYQKRKNV
                     RKLMEERLDKRTLKRLREIGNLADSLGYHAFLVGGFVRDLLLRIETYDIDIVIEGDGL
                     VFAEEMAKHFDIKVKPHKEFSTAKVTYSDGFKIDIATARLEYYRAPAALPVVEHSSLK
                     LDLHRRDFTINTLAISLNKNTFGLLIDFFGAQRDIKEKAIRVLHSLSFVEDPTRVFRA
                     IRFEHRFGFQIGKHTLSLIRNAIKMDFLSKLKGKRIWTELSLMMLEEAPEKILKRLQE
                     LNLLKFISPDLKFDREKERLFQQMHAVRQWYELLYKGKPCDTQALFVLGLLDRMSPEQ
                     VDDFCERTEMTESFRRRTVENTKRYRDAMAKLPLAIYTGKKSEIHKVLEMLSQETMLF
                     IMAKTKSEEIKKSISNYVTYKDVLRPTFTGEDLKRLGIKEGPIYREILETLKDAKIDL
                     NLKTKDDEETFVKKYIIDHNITV"
     gene            21068..21811
                     /locus_tag="PK125_03520"
     CDS             21068..21811
                     /locus_tag="PK125_03520"
                     /inference="COORDINATES: protein motif:HMM:NF014654.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HPB37210.1"
                     /translation="MNLIFPSLEIKMECYEGPLAVLITLIKKNKVSIWDIPLSTITER
                     FLQYVDLVTEMNLKIAEDFIEIASLLIYIKSRMLLPTNGEDKEEEDPRDELIDRILEY
                     ERLRVMVDSIDGLPMLDRDIFTRGKTVVEAETDFDLLALCSIFLEIMKSKEEKYIEIK
                     EIRPTIEEKLKMLKAILETSGIYVWNFDETIEQPEKIATVLSILELTKVRMATIVQRR
                     PFGKIIIRKRDDHGNIPSQRITNTSIVGK"
     gene            21897..22820
                     /locus_tag="PK125_03525"
     CDS             21897..22820
                     /locus_tag="PK125_03525"
                     /inference="COORDINATES: protein motif:HMM:NF024981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter"
                     /protein_id="HPB37211.1"
                     /translation="MPASIHFNDILLVFVVISSMALAVIFPDFGFRFRMLPYYCLMII
                     FFLSYLSIDIASIWTTLKSHSTEITVFSITKLVVLPVVFYFAFYFIAPSYALSALLLT
                     GVSVGVVAPMISNMVHGNSALVLVIVVITSVAAPFTLPAVVKIIASKDVTISFMSMLQ
                     MLATVIFVPIIVVEIIRHLLPRLVKPILKIRFPASLVLFAVINLGVFYRYASFFKNEP
                     WVILMSAIVVFVLAAIYCAIGIVFFRKSSLEDKLAGAVMLGNLNNVLIIVFSSEFFGP
                     VEPLVAAMYTIPFWVIVIPLRYYRNRMTRAI"
     gene            23315..23758
                     /locus_tag="PK125_03530"
     CDS             23315..23758
                     /locus_tag="PK125_03530"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HPB37212.1"
                     /translation="MPTKILVPTDFSTYSDKALRQAFDIAKQYNAKVYVIHVVHEKIT
                     DTIDDYGITYPSYVKEIEGKMIDEAKKKMKEQIDKFPQSKELEIESDVQIGNISEAIL
                     QEEKNKGIDLIVIASLGRSGIAKYLIGSVARNVLKGAKCPVLLTK"
     gene            24083..25459
                     /gene="ppnN"
                     /locus_tag="PK125_03535"
     CDS             24083..25459
                     /gene="ppnN"
                     /locus_tag="PK125_03535"
                     /EC_number="3.2.2.10"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: protein motif:HMM:NF038390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide 5'-monophosphate nucleosidase PpnN"
                     /protein_id="HPB37213.1"
                     /translation="MKYDTVDARISPEGRLNVLSKVEVNRLLDTSHGGLYRLFRNCSL
                     AVLNSGNYLDDGKELLERYRSFDIRIIQEERGIKLEVKNAPATAFVDGRIIRGINEHL
                     FAVLRDVIYTNDEIGCNPKLNLESSAGITNAVFHILRNANILRPTRNPRLVVCWGGHS
                     ISQLEYDYSKLVGYQLGLRGLDVCTGCGHGAMKGPMKGAAVGHAKQRINDGHYLGVSE
                     PGIIAAEPPNPIVNDLVILPDIEKRLESFVRIAHAVVVFPGGAGTAEEIFYLLGILMC
                     SDNANIPFPLIFTGPKSSKGYFNQINQFIGHTLGPEAQTRYKIIIDNPLAVAREVQAG
                     IRQVREFRKNTDDAYYFNWLLKIDTEFQKPFVATHENIKNLELHKNMERSLLASNLRR
                     LFSSVVSGNVKDEGIRIVEQHGPFEIHGDMEIIESLDSLLTSFVEQQRMRLPGTKYVP
                     SYRIIRKS"
     gene            complement(25590..25910)
                     /locus_tag="PK125_03540"
     CDS             complement(25590..25910)
                     /locus_tag="PK125_03540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB37214.1"
                     /translation="MKKALMLVLALLISVAFVTTVFAQAKPEAKPADKPAAVAAEKAP
                     APEKAVEKAEKPAVEKKAAKKVKKPAKKAEPKKEEAKKEEAKPAATPAPAPAPAPAPA
                     PAKK"
     gene            complement(26265..27065)
                     /locus_tag="PK125_03545"
     CDS             complement(26265..27065)
                     /locus_tag="PK125_03545"
                     /inference="COORDINATES: protein motif:HMM:NF024587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidylate kinase-like family protein"
                     /protein_id="HPB37215.1"
                     /translation="MAVLTISRQLGSIDREIPQTMARLWDYEYVDRRKILEDLSAQGS
                     QWEAFGKEFDEHYPNLWERFDRSFAGFVILSQSIILNYALKDNVIIVGRGGNFLLREV
                     PFALNVRIVAPIEQRLEEVMKRENLSRSAAELFVKKVDKEMSRAVHFIYGKNIDEPSS
                     YDLFFDVGVIPVHDMMGTIRAALLEKDKLKTDTAMEALQMKALAKKVRAGIVTHPTLF
                     IPVFDVIPGEGRLLVTGIVHNPDERKKVETEVKKLAGSVPVEFALHYR"
     gene            complement(27025..27810)
                     /locus_tag="PK125_03550"
     CDS             complement(27025..27810)
                     /locus_tag="PK125_03550"
                     /inference="COORDINATES: protein motif:HMM:TIGR03717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HPB37216.1"
                     /translation="MNFGSFGYFNFTWEFISGFLMVVLIDLVLAGDNAVVIAMAVRNL
                     PRKQRSLGIALGAGGAIILRVALTILVAQLLGIKGLKFCGGALIMWIALKLFIEGAPD
                     EGAKKEPKTIWQAMVTIVVADIVMSLDNMLAVAGASHGNNLLIIFGLILSIPFIVFTS
                     NLLSTLMDKYPIIVYLGAMVLGKVSGEMIITDPYVQSFLHTGKITQYVVEAAGAIAVV
                     LVGKLWIRMKVKREQEEESLVCTKHKEVASYGSLDHIEATRKY"
     gene            complement(28623..28802)
                     /locus_tag="PK125_03555"
     CDS             complement(28623..28802)
                     /locus_tag="PK125_03555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB37217.1"
                     /translation="MRYEDACPAYPDTEGFVVRKSEIKKVIRILIATNLYKALPARER
                     YDFLKYLLGKFTFSS"
BASE COUNT         8819 a         6229 c         6245 g         7742 t
ORIGIN      
        1 tcaacaggcg taagatggag gccaaaatta atatgtgaca taccaatcac aacaagaaca
       61 attgcgattg cagagcaggc aacaagtatg aaaaattgtc tatctgtcat aacttaccag
      121 gtatcttttt tgtaggttct gcctgggtat cgaacgtcgc tttcggaaat ttgaggattc
      181 ctttgagctc aaaagaagta gcacttgtaa cctggtcatc gcttctttga atccttattc
      241 ccgttattcc aaagaaagga aacttcaaag actgaaggta gccggcaaaa ttgacaaaag
      301 aagtataatc tttcagaaca gaacgaatta caacagaaag ctgcaggtca gcacccttat
      361 cctctatgtt cgcgaccgtt atttcagagg acccagcttt tgctttcaaa tcatcaaggg
      421 cgatcagcat atgctcctta tctgataacc ccgaagaatc atgaggaagc attgccttta
      481 cccttactaa tgacttttca atatcatcga tagcagttcg aacctttata tactttatgt
      541 tgaattcctg gagtttatca agcgttgcgt atagtgaatc aacatactct ttcatcgcga
      601 tagcacctgc cagggtcatc aacatgatta ttcctgtcag cccgtaaatg agcaaggact
      661 tccagagtct tcttttagtc tgttggaatg ttaacttcag tgcttccatg tcgtctctat
      721 gttaaaatcc ttactggtta aatcgagttt ctcaatcaga acttgcccac ttttggctga
      781 cttcatatca tctgccaatt ttcgaaaaca ggattgaagg tcaccgtatg tatttgctgt
      841 aatatgaccc ttgaccctga ttgtcagttc ttcgcccgga tttatgattt caatctcttc
      901 tactctcact ttatcgcgat tcaaaatttg aagggttgca agggccttct gaatatccgg
      961 ctttgctgac tctgcattca aataattgat atagggaaga actgcctgaa ggtccttgtt
     1021 tcgtgattcc aattcttcat aaacccttct tttactgcca atttctgcct tgataggatc
     1081 tatcattcca cgtatttgac gcacctggtt ccatcctgag ataatgtagc caagacataa
     1141 aagtgaaacg atcacgaagg ctactgaggc ataccgcata aaatgctcct gtgatactac
     1201 acgctgatac agtttcggaa gaaggctttc ccttacatgt ccttttccca gaaataatcc
     1261 agccacaggt attacaaacc cggttatgtc cccccctgtg gccataaccc ctggcggata
     1321 tcggaagatt gtcgtcggga gtgttaatcc ttctacggtg gaaacctcat cttttgcagt
     1381 gtttactata atgagttctt ctgggtgttc acgcagtgtc tggcgggcat agttgactgt
     1441 catgttgaca ttggttacat cataccggtc tatacccctt ccccgcgact gaatactcct
     1501 cacaaaagtg agctttccct cccttacaag gaataaagtt ttgtaatccc ctttgtcgat
     1561 catacacatg gctgtcttat ccttcacatt atccagacca acaacaagac aggagagaac
     1621 aaaaacatca gcatacagac gcttgaccct ctttccgaat ctactgaacc tttcaaggat
     1681 agggtttatt tcggtggaat ccactgcgaa aacaaacgtt tccctcacag gtctcccttc
     1741 gtgaaccttc tcttttaaga ttgtggaaaa gaaaacaaag ttcttgagtt caggtgcatt
     1801 ttttttaatc tctgcctcaa tcagggtgtc aacgtatttc atctcaactc gtggcaaaag
     1861 aaggacatcc tgataaaagg tctgaaagct gaaaaggaca atgaaatcct tggtcttttc
     1921 acgggctaaa aaggcatcga attcgtcttc cttcactgtg aaggttcgcg taataaccaa
     1981 agttccattt ctttgggaac cgtaggatac tttggcatac ccactttcaa aacttacagc
     2041 tatctgttta ctcaacagtg ttcaccagga atggaagttt attttgagaa ctgtttgaat
     2101 caaacttata ccgagtcctc ttggaagagt caagattatt gttatgtcgc aatttctgtt
     2161 ttttatgata cacttatcaa ctgtcatgaa aaactatttt ggggagcggg gcttaacact
     2221 catcgagatg gccatagttc tagtgattat tgggcttctt gtgtctgttg gcgcagcact
     2281 catagggccc ctgacaaaaa gggcaaagct cacagaaaca agggagattg tcaaaaacgc
     2341 attcgaatca atccttggtt tcgccatagc caataagaga ttaccgaatc aattgtctga
     2401 cctcggcatc aaaaccagtg atgcatacct caataatctt atttattaca aagcgtatgc
     2461 tgatacacct cccagttaca acctgtgcac atctgttggc acctacgtaa ctgtcaacga
     2521 ccagggagtg ggaaaaacaa atgtggcatt tttagtcttc agtgccggag aaaaccgtgt
     2581 aaacaataca ggcactgctt caccattttc catacagata ccgggggtgg gcggatatga
     2641 tgacgtagct ctttatatgg atataggcac gttacgccag aaagcatgta acgtactccg
     2701 tattgttaca gatagcctgc caacagctac agaagagacc gtatatccaa cctctgcact
     2761 tgaggctact gatggcacgc ttccgtacac atggaccatc acatccggtg ctttacctcc
     2821 cggtcttacc ctaataccag gcacaggagt tatagccggc actcctatca atgatggaag
     2881 ctacaaattc accattcagg taacagatag cgataacccc caaagaacag cctcaaaaag
     2941 cctggcaatt actgtgaacc ctgacaaacc acggattaca actgaatttc ttacctatgg
     3001 tacagtaggc cagccttacc caagcagcac cttgagtgcg agtggtgggg ctgcacccta
     3061 ttcgtggact cttgcagggg gaagcaacct ccctcccggg ttatcgctct cctctggagg
     3121 aatcatttca ggaagacctg ccagtcctgg aacatttgcc ttcacagtta ctgtaattga
     3181 tggtcgatca agaatggcaa cgaagaatct ttcaattgca gtctacccat agggagacag
     3241 cataaactca atgagcaatc ccaactgaca tcgacatcat tatcatcgaa caacaggggt
     3301 agcatactac tgtcatgttc cctctgggca agccctcgca ttacccaatc tgggcagaca
     3361 accactttga gctgacatat ttcgagcagc ctcaggaagt agggcctctt ctccctcaaa
     3421 tagcacatgt acaagcccta ccaaaaaaag tttttattat tttatgtttt tctggtgaag
     3481 attatccgat accgttcagg gccagcatcg taacaggcag tacatccact ttcctcacag
     3541 cttgcctggt caatttcgtc tttggatggg agaggctcaa gatggacctc aaaacctatt
     3601 tccttataca tttccaccat ttctgacagc cttggctcgc atgcgataaa ccttctttcc
     3661 catccctctg ccacaagttg ttcgtcccgg ttcatataaa aaccttctct atttctctat
     3721 cttatctaca ctatctgtta tttctgaaat attctatcgt catttcaagt ccctcatcaa
     3781 ggcttatctt cggtgtccaa tcgaggagtt gttttgcctt tgcaatatca gggcatcttc
     3841 ttttcggatc atcctcggga aggtcttcat atactatggc agacttcgac tgtgaaagcg
     3901 ttaccacctt ctgagcgatg tctttgatgg tgacctcgtg agggttcccg atgttgattg
     3961 gccctggcat gcctgagagg tggggcttgg tgtagtccct gcttttttta tgtgagcctg
     4021 tttcgaaatc catcatgcgg atcatacctt caatcaggtc gtctatgtag cagaacgacc
     4081 tggtttgcaa tccgtcgcca tagatagtga tgggcttgtt gtgaagagcc tggataataa
     4141 agttacttat tacgcgtccg tcgtcagggc gcattctcgg gccataggta ttgaaaatcc
     4201 gaatgacctt tatgtctacg ctgttctgtc tgagatagtc aaagaagagt gtctctgcaa
     4261 ccctcttgcc ttcgtcgtag cagcttcttt tgccgatggg atttacgctc ccccagtact
     4321 cttccttttg cgggtgtact tctggatcac cgtatacttc actggtggaa gcctggagaa
     4381 tccgtgcccg aaccctcttc gcaaggccga gcatgtttag ggctcccaag acattcacct
     4441 ttgttgtctt cacgggattg tactgatagt ggatggggct tgcaggacag gcaaggttaa
     4501 agatccagtc cacctcgagc aggatgggct gcgtcacatc gtgggtgata agctcgaaac
     4561 gggggtttgg gaggaggtgg aggatattct gcttggttcc cgtaaagaaa ttgtcaaggc
     4621 acaagacttc gtggccatct gatatgagcc tctcacagag gtgagagcca atgaatccgg
     4681 cacctcctgt tacaagtatc ctctgagggg ttttatatga tctgaattta tacacggggc
     4741 ctcccgatgc agatataacg gaagccgtgc cgcgccattg ttttcgggtt gtactggttc
     4801 ctcccatcaa aaatgaccgg ctctttcatc aggcctttca tcctctcaaa atcaggttct
     4861 ctgaaggaca accactcagt gacgagtata aggccgtcag caccttccag tacctcatac
     4921 tggttcttgc cgaattgtac attctgattg cccgagaaca acggttttgc cgaatccatg
     4981 gcctttgggt caaatacatg gcacagagcc ccctgtccgg tgagggtttt cacgatatac
     5041 tgggagggag cctctctcat atcatcggta ttcggtttga aggctaatcc ccagacagca
     5101 aacttcttac ccttaatgtc attcttatag aagtccttca ccgcctcggt aaaggatacc
     5161 ttctgattct catttacatc catgacactt tcaagaatgg atgggttata gccgagatct
     5221 cgggaggtct ttacgagggc acggacatcc ttggggaaac aggatccccc gaacccgaca
     5281 ccgggaaaga ggaattttgg gccaatccgt gaatcactcc ccagacccct catgaccatc
     5341 atgatatcag caccgagcct atcgcagata tttgccatct ggttcatgaa ggatatcctc
     5401 gtcgcaagca tggaattggc tgcgtatttt gtcatttcac tggacctgac gtccatgacg
     5461 atgaagggag caccggtccg tgtaaaaggg gcgtagagct ccctgaggat ttcttccacc
     5521 ctgtcatgtt cgacaccgat gatcacccgc tcgggcttca tgcagtcatc aacagcaaca
     5581 ccttctttaa gaaattcagg gttggaggca atgtcaaaag atacattacg gcctgccttc
     5641 tcaagctcct cctgtatggc agccttgacc atatcacagg ttcccacagg aacagttgac
     5701 ttgacaacca caatccggta cttttccatt gctttcccga tatcgtgggc aaccttcatc
     5761 acatatttca aatcagcgga cccgtcgtgg tcctgtggcg tatttacagc aataatgagg
     5821 atgagtccat gatcaacagc ctcttttata tctgtggtga accgaagcct tccttcctta
     5881 atgttgttct ttaccagctc ttccagttga ggctcgtaaa tggggattat cccctccctg
     5941 agcttgttta tcttttcact gtccacatcc atgcacatga catcattgcc tgtctctgca
     6001 aaacaggaac ctgataccaa gccaacataa ccgaccccaa tcacagaaat gcgcatatga
     6061 cctccaaatt ttctttattt accgtgcatt actcaggtaa gtcaacatct attgtgtgaa
     6121 gcaaacctcc atggtaaaat aatctaccct atcaaaagct agcaaaaagc ataccaaaca
     6181 tgatgactat atctccagac aagaatattc ttcactgaga tttgttcatc tggctctcct
     6241 cttccatctt gcgccccttt tatactctat tatctctttt ctttctcaaa gtctcttgtt
     6301 ttgttgcctg tcacccatgg gttatcatct ccaataagtc aatatttatt gctatacaaa
     6361 cgatattatg ttaacatatt ccacgaggtg ggcttgaccc gcttttttat tatcatgacg
     6421 tatgataccc caacgggaaa taatagagca acttcaagat atactcttgc ctgttcttca
     6481 ggattatgct ttagagcttg ttgagatcga atttaagccc tcaggaaaaa gatggctgct
     6541 gcggttatat attgacaaag aagggggggt tacgattgca gactgtgaaa atgtgagcag
     6601 ggagtttgga aggatcctcg acgttgagga tattatcgat cacccttaca gtcttgaggt
     6661 ttcttccccc ggactgacaa ggccattgaa gaaatgggag gatttccaga gaaacagggg
     6721 aaaactctgc aaaatagtaa caagagaaaa gattgacgac aaaaatgaat tccacggaac
     6781 aatcatggat gccagtgagg atacaattga aataaaagga acaaataatg tatttaccat
     6841 cccaatatat gctataaaaa aagcaaattt ggagtttgaa ttttaagagg ttatcatgta
     6901 ttttgactta aactacatta ttgagcaggt tggaaaagaa aagggcatcc caaaagaaac
     6961 acttatcgaa gcccttgaag aagcaatcct gattgcatca aagaaaaagt ttggaaatca
     7021 tctcgactta gaggcccgct ataacgaaga actgggtgaa atagaggtgt tccagtttaa
     7081 aacagtggtg gaggaagtca ctgagaatga tatggaaatc tctcttacgg acgccaaaaa
     7141 tcatgacccc gaatgtcagg taggtgacag tatcggtata aaaatggata caagttctct
     7201 tggcaggatc gcagcacaaa ctgcgaaaca ggtcatagtc cagaaagtta ggaacgctga
     7261 aagcgatgtt atctacaatg agtacaaaga caaaataggt gaaatatcga caggagtagt
     7321 ccagcgaatt gaaaaaaatc attacataat taaccttgga aaaacagagg ccatactccc
     7381 catgaaagag ataattccag gtgaagcatt caggcagagg gacagggtta aggcctacat
     7441 ccttgatata gagaaaactc agaaggggtg caccattcat atgtcccgga cacatcccaa
     7501 ttttctggct aaactctttg aacaggaagt gccagaaatt caggaaggta tcattaagat
     7561 tattggtgct gcgcgcgaac ctggagaacg tgcaaaaatt tcagtttaca gtgaagatcc
     7621 agatgtagat cctattggtg cctgtgtggg agttaaaggg tccagggtgc agaccgttgt
     7681 ccaggaacta aggggcgaaa agattgatat cattcctttt agcaaggatc ctgcaaaatt
     7741 tgtgtgcaat accctcgcac cggcaagggt atcaaaagta tacttaaatg aagaagagca
     7801 cgctatggaa atcatagtca acgatgatca actttctctc gctataggca agaaagggca
     7861 aaatgttcgt ctcgcatcac ggctgactgg atggaagata gacattaaca gcgagtctga
     7921 attagagctg acttcaagga aggttatcga cgaattgatt gaaaatttga agataagcga
     7981 gattcttgcc agaatcctct atgatgaata cttgagagaa ccacacgata ttgcaaagct
     8041 cacacctgaa gaaatgaaca agataacgag catatccatc gatgactgtg ttcggattat
     8101 tgaacaggca aggttgatca gggatataga atctcgtgaa aaggaggctg aagcagttac
     8161 cgaaaaggaa aacatcgttg ttccggcgct gaaagatgac gaagcaaccg atcaagcttc
     8221 aacacagtaa ttaaattttt tgttaaacgc aaggaaggac agaacatggc aaaagttaaa
     8281 atcaccacac ttactgacaa aataagtgat gatgagattc ttacaaggct taaaggcctc
     8341 ggtgtaaaaa tcaaagataa agcaaaagaa gaacctaccc ttgcaaaaaa ggatgacgaa
     8401 aaattgacat cttccggcga aactatggtg gaaaaacggg tagcatcaac gataatacgc
     8461 aggcgcgtca agccaccgca accccaaaaa gagaaagcag aggttgtaga agaagaagta
     8521 aagccagagg aaaaaaagcc cgaaaagaaa gaaacggtcg tcaggagggc ttccagaaag
     8581 gctgaaaagg ttgaggaaaa gaaggaaatt gttcaacctc aggatcaacc tgaaatcatc
     8641 aagatagaaa aaattgaaaa aaaagaagaa catgttgccc ctgtattgac tgaacgtgat
     8701 gaggacgaag aacaaaacga agaggcttta aagggcgaca tcgagatcat cggcgaagag
     8761 aaaaagaaag agataacaaa ggtacttgaa gaggcttata aacaggatct cgaaaaagat
     8821 tttacccttg ttgagtccga acctgatgag ggaatagagg aaaagaaaaa gaggaaaact
     8881 gaaagacttc ttaaaaagat agaagaacag gatgcagagg agactaagct taagaaaaaa
     8941 ggaatcctca agagaaaagt cgtcatcaaa gaggaagatc tctatacctt tagaaggcag
     9001 aaagcccgtc ctatacctgg caggaaagac aggaaggaaa aacctgaaga gaagaaaata
     9061 gaggaaaaag tagctgagct taaacctaca aagaagatag tcaaagttgg ggaagaaatc
     9121 cagattggtg aactcgcaaa acgaatgaat gtaaaagccc aggatataat caaaaaactt
     9181 ctcccccttg gcataattgc aacagtcaat caagacattg attttgatac tgcttatctc
     9241 gtaggaacag aactcggttt tgagattgaa aagatcatct ccattgaaga ggaatttttt
     9301 gcccgcgaag aagagggcaa ggagaatgaa aagaatctcc atccaaggcc acctgttgtc
     9361 actatcatgg gccacgtaga ccacggtaaa accttacttc ttgataccat caggcattca
     9421 aatattgccg aacgagaagc aggcggcatt acccagcata taggggccta tgtggtcgac
     9481 gtaaatggaa aggaaatcgt atttgtggac acccctggcc atgaggcctt cacagcaatg
     9541 agagcacggg gtgcacgtgt tacagacatc gttgtcctcg ttgtagcggc agacgacgga
     9601 gttatgcctc agacaattga agccatcaac catgcaaagg cggcaaatgt tccaattatt
     9661 gtagctatca acaaaattga cagacagaat gcgaatatcg acaaagtttt gaaggagctt
     9721 gccgaacagg ggctggtccc agaggagtgg ggcggcacca cattgttggc aaaaatatcg
     9781 gcaaaaaaga aaataggcat tacggagctg ctggaactaa tcatactgca ggcagaaatg
     9841 ctcgaattaa aggccaatcc tgatatgcgt gcaagtggta tcatcattga gtcagagttg
     9901 gacaagggcc agggtcccgt gggtacggtc atcatccagg aaggcacatt gaaaatacag
     9961 gatcccttta ttgccggcac aatgttcggc agggttcgcg caatgataga tgataaaggt
    10021 aaaagaatcc aaaaggcacc tccctcgaca ccggttcttg ttgtcggatt tcaggacgtg
    10081 ccccatggtg gtgaccggtt tatagccacc actgaagaac ggtatgcaaa agaactgtcc
    10141 cgctatcggc aggaaaagct tcgagagagg gagactgcaa agagttcaag gactaccctt
    10201 gaagaactct attctaagat gggtaagtca gaaaaaatac agcttggcgt tattataaaa
    10261 gcagatgtga gaggaacaat agatgccata acagaggccc tcaaaaaatt gtcaaatgat
    10321 ctcgttgaaa ttcagataat tcatggtggt gcaggagcaa taaccgagac agatgtaaac
    10381 cttgccatgg cctcaggtgc aatcataatt ggttttaacg tgaagcccat tggaaaagcc
    10441 caggctcttg ccgagcagga aaagattgaa ataagaacgt attcgatcat ctatgatatg
    10501 attgatgatg tcaagaaggc tatggaaggc cttcttgaac ctaaactcgt tgaaacggac
    10561 attggaaaag cagaggtgag aaaagttttc gcagtatcga aaataggtac tgtagcgggt
    10621 tcatatatct tggaaggaaa ggtaacacga aatgcaatgg cccgcgttat gaggaacagc
    10681 aaccatctct ttacaggcaa gatttcctct ctcaagaggt ttaaagatga tgtaaaagag
    10741 gttcaggcag gttatgagtg tggtatatca cttgaaggat ttaatgattt gcaagaaggc
    10801 gacagccttg agttttttgt tcaggaaaaa gaaaggcaga ctcttgatgg ttgaaacaga
    10861 tgatcgtcgg tatttctacc atagaaatat tctttcctga aaaccaatcc ttaaaagata
    10921 aaaggcaggc actgaaaaaa attgtcgaaa agacacggac aaaattcagt atttcgatta
    10981 tggagataaa tcagaataac ctctggcaaa gggctcaaat aggattttct gtggtaggag
    11041 taaaacgtga ccacgtttac agaatgattg acaatgttca cgaatatgta gaatcccttt
    11101 acattggcac aataatcgac accaagacag aaattatcgt gatgggtaat gagatataga
    11161 aagctgcgtc tccaggatca attacgtgaa gaaatatcct tcatcattca acgggagata
    11221 aaagaccccc gtgttggatt tgtaacgata ctggaagtaa agatgtcaaa tgatttgaag
    11281 atcgcaaagg tgtattgttc ggtatatggc gaagatgaag acaagaagaa gactattgaa
    11341 gcgcttcaga gatcaaaggg ttacattaaa ttccgcctcg gaaagaggat tcaactgaag
    11401 tacatgcccg atctaacatt tctcattgac aacacctttg aaaaagcggc acgcatagaa
    11461 gagatactca ggaaggaagt ttatgttcag gaagattaaa cagataatcg atggaggcaa
    11521 caaatttctc attaccacgc acattgaccc agatggagat gcaattggtt cctgtttttc
    11581 tctttattgg gcccttgatg ggcttcaaaa aaatcctttt gtttatatgc gagacagagt
    11641 gccgtatatg tatgaattcc tccccaaacc aacacaactg ttttataatg aacccccgga
    11701 tgggacatat gatggtattt tcatccttga ttgcggaaat ttttttagag ttggcaatgg
    11761 ccatgagaga ttgaaatcgc aaggaccgat tataaatatt gatcaccatg ccacaagcga
    11821 gaagttcggt atcataaata ttatcaatca aaactcatca tctacaggag agatcctcta
    11881 taatatatat gatgcattga tgatcccaat ttcctataac attgcggtaa atctctatac
    11941 cgcaatcttc actgacaccg gttcttttag atatcccaat acgaattctg aggcatttct
    12001 catttgtgaa aagatgacaa aattcggtgt ctctcccgct catgtagctc agatggttta
    12061 tgaaaaccac cccaaggaac gattcgtcct cctcggagag gttctttcat cgctcgaaac
    12121 ctacgaaaac gacaccatag ccctagctgt ggtaactaag gagatgttcc acaaaacaaa
    12181 tactgccaaa gaatacaccg agggttttgt ggaatttatc aaagagattc gcggggtcaa
    12241 tgttgcgatt cttatgaaag aactatctga taatcaatac aagataagta tgaggtcgaa
    12301 aggcgataca gacgtagccc tgatttgcaa tcactttggg ggaggtggcc accggaacgc
    12361 tgcaggttgc acaataaacg gaagcattat ccagataaaa gacaggctaa aggaggcact
    12421 tacaaaaccg tgaatggttt tctagtgata gataagaata cgggcatgac atcctatgat
    12481 gttatacgga gattaaagaa gctccatcca ttcaacaaga taggctatat aggcaccctg
    12541 gacaggaatg caactggtat cctgcctgtt gcattcaata atggtgtgaa gttgataccc
    12601 tttcttgaaa acagcgagaa gacctataga gccacgtttc tgctcggtgt taccactgac
    12661 actctcgaca ttgagggcaa ggttttgagc agtgttgatg taaaggagtt taaaagagac
    12721 atccttgcga gagaattgaa caagtttaaa ggtaaaatcg tgcaacaaat ccctgcgtac
    12781 tcgtcaaaaa aagtcaatcg caagccatta tataaacttg caagaaaagg tattgcagtg
    12841 gagcctcact tgaaggaggt tgagattttc agcatcaagt ttctcgatta caatcacccg
    12901 tacctggatg tggaagtcac ctgttcaaag ggcacttaca ttagggctct atcccaggaa
    12961 tttggagcaa acctcggttg cgggtctaca ttgtattcac tgaagagaac caagcatggt
    13021 gagtttactg aggatatggc agtaaatatt gaacatttca aaatagaaga agacgtgtta
    13081 cactatgcta tgtcactcga agcaattcta aagacactca gggagactgt agtcgaaaca
    13141 gcccttgaga aatttctgaa acacggtatg cccataccgc tatccggcga tacaaaagaa
    13201 tggcagaacg gagaatatac aaagctgcta aacaagaaag ggaatctcat cggcatcggt
    13261 atggctgata cattttcaaa aacgataaaa attaaaaggt taatcaatat ttaaggagga
    13321 attaatggca ttaacaccga cacagaaaaa aaatatcatc gatggatatc gcatccatga
    13381 aaaagatacc ggctctcctg aggtacagat agcgctcctt acagaaagaa taaaatcgct
    13441 caccgagcac ttcaaaaaat tcgggaagga ccataattca cgaaggggtt tacttgtgct
    13501 ggtaggcagc aggagaagat tacttaccta tttgaaggaa aagaacgtcg acagatataa
    13561 aaagattatt gagagactcg ggctcaggaa ataaaaacat ataatatcag gaaggaaact
    13621 ccatggaaga acagattacc attgactacg ccggcagaca attgacaatc agtactggtg
    13681 cccttgcaaa acaggctgat ggcagcgttg ttgtgcgata tggcgacgct gtcgtacttg
    13741 tgacagtagt tgtcgacaaa agggcatcag caaaagattt tctaccgtta actgtaaatt
    13801 accacgaaat gtcatatgca gcaggcaaat ttcccggggg tttcttcaag cgcgaaggaa
    13861 ggccgtcaga aagggaaata ttgatgtcac gtcttattga cagacctctg agaccgcttt
    13921 ttcccaaagg atttaccaac gaggttcagg ttattgccac tgttctctca gcagatcagg
    13981 aaaatgaccc ggctattatg ggaatccttg gcgcttcttg tgcactcacc atttctgaaa
    14041 ttccctttga cggacccctc gccggcgtta aagtgggacg taaaaatggt tcttttgtta
    14101 tcaaccctac cccccttgac cttgaagaaa gcgaactgga cgtagtcgtg acaggaaaca
    14161 aggaagctat tatcatggtt gaagggcaag caaaatttgt aaactgtgaa gaccttatcg
    14221 aggcaataca tttcggccat cagaaactta ttcctttaat agaaatacag gaacaactta
    14281 aagaaaaagt gggcagacaa aaatgggaag ttattgttga tagcaggctc gatgctcttg
    14341 tacaggagtt aaaaggaaag gcggaagagg atatcctcaa ggctttctct gcttccggaa
    14401 aacaggaaag atcaaagaaa cagaacgatg tttttgatgg attgatcagc gtatacccgg
    14461 acatcgaaga agccctcatg aaaacggcct ttgaggaaat tactcgtgac attttgagga
    14521 aacagctctt tgccacatca aggaggattg atgggagaac atgggaagac atacgtcctc
    14581 tatcatgcca ggctggtatc ttacccagaa cacacggctc tgcattgttt acaagaggag
    14641 agacccaggc tcttgcactt actaccttcg gaacatctga agatgagcaa aaggtagaat
    14701 cgcttatgga gggagaaacc ttcaaaacct ttatgcttca ttataacttt acacctttct
    14761 ctgtaggcga ggtagcgatg ctgaggggac cgacacggcg cgagatagga catggcaacc
    14821 ttgcggaacg cgccctcacc cccattcttc ctccaaagga agactttcct tatacaatcc
    14881 gaatagtttc tgaaattctg gaatcgaacg gttcatcatc gatggccact gtgtgcagcg
    14941 gttgcctttc cctaatggat gccggagtcc ccattaaaga gcctgtagcc ggaattgcca
    15001 tgggtcttgt caaggaaggt gataacgaaa tcatcctttc tgatatcctc ggcgatgaag
    15061 accatctggg agatatggat ttcaaaattg ctggaacaag ggacgctatc accgcaattc
    15121 agatggatat caagatcaag ggcatttcta aagagactat gcagaaagcg gttgtccagg
    15181 gtcagaaggg aattagaacg atcctggatg caatggcaca aacccttgaa aaaccgcgtg
    15241 aaacaatgtc tccctatgca ccaagaattt ttaccattcg aattaaacct gacaagattc
    15301 gagatgtgat tggtccgggt ggaaagatta tcagaggtat tgtggagcaa accggggtaa
    15361 agatcgatat agaggatgac ggtatggtaa aaatagcctc tattgatgaa gggtctgcaa
    15421 accaggctat tgatattatc aagaaattga ccaaggaagc agaacttggt gagatttata
    15481 caggtaagat aaagaagatt atcgattcag gagtaatcgt ggagatactt ccaggggttg
    15541 agggatacgt ccatgtaagc cagctcgctg agggttatgt caaaaaggcc actgatgtgg
    15601 tgaaagagcg ggaagagata actgtcaagg tcatagagat tgaccctacc aatggaagga
    15661 ttaaactttc aaggaaggca gccctaaagg aacagaaagc acaggaataa ttccaaatat
    15721 ctataaatgt acaggaaaac aattcttgaa aacggtctta cagtagttac tgaatcaatc
    15781 ccttattttt ctacagtatc actgggtatg tggtggaaaa caggggggag atttgagaac
    15841 gaagcaaaca acggtatttc tcattttatc gagcacatgc tctttaaagg taccctgaaa
    15901 cggtcggcct acgacattgc caaggaaatc gatgctgtgg gtggagccat caatgccttt
    15961 accggtaagg aatatacctg tctctatgcc cgcgtgttaa gaaaagacac tgatatagcc
    16021 ctggatattc tctctgatat gtataagaat cccctgttcc aggaagacga ccttgaaaag
    16081 gaaaagtatg ttatcgccca ggaaataaaa atgattgagg acagccctga agagtatatt
    16141 tacgagatgt ttaatgcctc atattttaaa ggccactcgc tcggtcttcc catcctggga
    16201 aaacaggaga atgtggagaa attcacccgc catgagctac gtgaccattt tcaccgacat
    16261 tatgccctgt acaacctggt aattactgct tcgggcagaa ttgatcatga tgtctttgtt
    16321 cagaaggttt caaaattctt taattgcaat caaagccatg gaccaacgcc agaaacaatg
    16381 gcagtaccaa gcccttgtgc aggtttccat atttatgaga aggatcttga gcacgtgtat
    16441 ctgtgcattg gcacagatgg agtaagtcag gttgataaca ggcgctatag actctatctt
    16501 ctgaatgcca ttatgggggg aagcatgagt tcccaccttt tccaggaggt tcgtgaaaaa
    16561 agaggccttg tttataatat ttactcttat gtgaattgct accacgatgc aggaaccttc
    16621 ggaatttcaa catcaacatc ccatgaatct gttgaagaag tcctcaccct gataaaacag
    16681 gaaatgatca gaatcagaga tacggggatt accgatgctg aactgtcttt ttcaaaggaa
    16741 cacctgaagg gcaacctttt tatctccctt gaaggctctg aaacacgtat gggcagactt
    16801 gcaaaaaacg agatctattt tggcaattac atacctttga aagacaccct gcgagaaata
    16861 gacaaaatca ccaagtatga agttgatcaa atcggtaatt caatcttcaa aaaccctgaa
    16921 aacatatccc tgaccatact tggtaatgtc aacagggagc agatagaagt gctatggaaa
    16981 ggttagaggt tgttatcaaa aagaaggatg gtgctatcct tccttcttat ggcactgagg
    17041 catcttcagg gcttgacctt gcggcctttc tcgataaacc tgtggtactt gcaccattcg
    17101 cacgggcact gattccgaca ggaatttaca tcgctattcc cgaaggtttt gaggcagaga
    17161 tacggccacg aagtggtatc gcccataaat acggcgtcac tgtgcttaat actcccggca
    17221 ctattgactc tgattatcgt ggagaagtca atgtattgct catcaatctt ggtgatcaac
    17281 ctttcaccgt caacaacggt gacagggtgg cacagattat cttcaagacc attgtgacca
    17341 tcgactggag agtaacggag gaactccctg acacaaaaag gggtcatggt ggttttgggt
    17401 caacagggaa atgatgagag ttaatggttc aaagctcagg tttctgtgtt cagcacgatt
    17461 cagccaatcc tgttttcgtt tcaccccttt aaatgtcagg atatttcaaa caaaactcca
    17521 aactcacatt cccatcctgt attttgtggt atggtacctg gcatgaacct ttactatgcc
    17581 ctcgacaact tcatcactta cctcactgca gaaagaggcg catcacccca cactgttgat
    17641 gcctataaca gagatataat cagctttatt agatttcttg aagagaaagg cctttccctc
    17701 tctgaaagga ctgaaagatc cttcattgaa atttacatag cccacatgag ggagcttgga
    17761 aagaagacaa ggaccattgt tcgtgcaatt tctgcattaa gaggtttatt caattttctc
    17821 cttgcagatg ggattattac ccataaccct ctcgaagata tcgaaatacc aaaatattct
    17881 ccccctgttc ctgaggtttt gagtgaacaa gaagtattag agcttctcca gcttcccaat
    17941 gggtcaaaaa cctcactaag agaccgcatg atccttgaac ttctttatgc aaccggtttg
    18001 agagtctctg aactgatcaa attgaaaaaa agtgacatca acctcgatgg ggggtttgtc
    18061 atagcctcgg gcaagaggtc caaggaaaga gtggtccccc ttaatatcta ctccaaaaat
    18121 acgataaagg cctatcttga agaagaaaaa ccccaggggg cttatcttct accaaacaaa
    18181 cggggtggca tgcttaccag acaagccata tggaagatta tcaggaagta tgctacaaaa
    18241 atggcaagag acaatgtgtc accacatact atgaggcata cctttgcaac ccatctcctt
    18301 gaggggggtg ctgatctgcg atcagtacag atattgcttg gccatgaaga catttctact
    18361 acccagatat atacccacgt ggacagcaag aggttaagag aaattcacaa aaaacatcat
    18421 cccagaggct gatgaaagtc ataacaagcc atcacaatgc tgattttgat tcgctctcct
    18481 caatggtggc tgcaaaaaaa ctgtaccctg atgcccttct tgtctttccc ggttcacagg
    18541 aaaaaaccct cagagatttt ctgattcatt ccacccttca tgtatacgac atagcaaaga
    18601 taaaaaatat caattatgat gaggttgata ccctcattct tgtggataca cgtcagaaat
    18661 caaggattgg ggacctcgca aaggttgttg ataaggatag cgtaaaaatt cacatttatg
    18721 atcaccatcc cccttcaagt gatgacataa agggggatct ggagattata aaaaacactg
    18781 gtgcaactgc gacaattatc atccatatcc tgaaggatag aggtataccc atatccccag
    18841 aagaagcgac catcatgatg ctcggcctct atgaagaaac aggaagcttt cagtaccctt
    18901 ccacaacaac ccaggatttc gatgcagcct ctttcctatt gtccaaaggg gcaaatgtga
    18961 atcttgtggc agatatgctt gtaaaagaac taacacctga gcaggttttc atgctcaatg
    19021 atctcatcag taatgcaact gtatacagta ttaacggcgt tgatattgtc attacagaag
    19081 gctctacaga aaagtacgtt ggagacctcg ctgttgtggt ccacaagttc cgggacatgg
    19141 agaatatcaa tgttgttttc gcacttttca ggatggagga caggatctat atcatagcgc
    19201 gaagcaggat ccctgaggtg gatgtgggtc atatcctttc tctacttgga ggcggtggcc
    19261 acaaagaggc agggtcatcg accatcaaag acatgaccat cattgaggca caggaaaaac
    19321 tcctcaaata tctcagacac aatgtgaagc ccctctggaa agccaaagac attatgttct
    19381 ttcctgtcaa gtcaattgat gcacgttgtc ccatcagcga agcaaagagc atcatggtga
    19441 aatataatat caatgccctg cccgtcctga atgaagaccg gattgttggg ataatttccc
    19501 gtcaaattgt ggaaaaggca gcctttcata aacttgaaaa tctacctgtc caggagtata
    19561 tgtctactga tgcctccaca gtcaaatcgg atgactccat cgaaagggcg aaggagatta
    19621 taattggagg aaatcaaagg ttcctgccgg tgacaaaaga tggaaagctg gtcggtgcaa
    19681 ttaccaggac tgaccttctg agagtgcttg aagaggagat atccaaaagt gttcgcggaa
    19741 agatggaatt ccaagaaacc tatcagaaaa ggaaaaacgt tcggaaactc atggaagaac
    19801 gtcttgacaa aagaacatta aaaaggctga gggaaatagg aaaccttgca gattctctcg
    19861 ggtatcatgc ctttttggtt ggtggtttcg tgagagacct tctcctccgg attgagacat
    19921 acgatataga tatagtcatt gagggcgacg ggctcgtgtt tgctgaggag atggctaagc
    19981 atttcgacat caaagtgaaa ccacacaagg agttctcaac agcaaaagtc acttactctg
    20041 atggcttcaa aatagacatt gctacggccc gactcgaata ttacagggcg cctgccgcac
    20101 ttcctgttgt cgagcatagc tcccttaagc tcgatcttca caggcgcgat tttacgatca
    20161 atacactcgc catatctttg aacaaaaaca ccttcggcct tcttattgat ttttttggtg
    20221 cccagagaga catcaaggaa aaggccatca gagtacttca tagcctgagc tttgttgagg
    20281 atcccaccag ggttttcagg gcaatcaggt ttgaacaccg gtttggcttt cagattggca
    20341 agcacaccct tagcctaatc agaaatgcaa taaaaatgga tttcctctcc aagctgaaag
    20401 gcaaacgaat atggaccgaa ctctccctca tgatgttgga agaggcccct gaaaaaatcc
    20461 tgaaacggct gcaagaactc aatcttctta aattcatctc acctgacctc aaatttgata
    20521 gggaaaaaga gagattattt caacagatgc atgccgttcg ccagtggtac gaacttcttt
    20581 ataaaggtaa accctgtgac actcaagccc tctttgtcct tggtcttctt gaccggatga
    20641 gccctgaaca agttgatgat ttctgtgaaa ggacagagat gacagaatca ttcagaagaa
    20701 gaactgtaga aaacactaaa cgatatcggg atgctatggc aaagttgcca cttgccatct
    20761 atacgggaaa aaagagtgag attcacaagg tgcttgaaat gctttctcaa gaaaccatgc
    20821 ttttcattat ggcaaaaaca aagtcagagg agataaaaaa atcaatctca aattatgtga
    20881 catataaaga tgttttaagg ccaactttca ctggcgaaga ccttaagaga ttaggtatca
    20941 aagagggtcc catttatagg gaaatcttgg aaactttaaa ggatgcaaag atagatctaa
    21001 acctgaaaac aaaagatgat gaagaaacct tcgtaaaaaa gtatattata gatcacaata
    21061 ttactgtatg aaccttatat tccccagcct tgaaatcaag atggagtgtt atgaaggacc
    21121 actggcagtc ctcataaccc tgattaaaaa aaataaggta agtatctggg acatccccct
    21181 atcaaccatc acagaacggt ttctccagta tgttgacctt gtcactgaaa tgaacctcaa
    21241 aatagctgaa gatttcattg aaatagcctc ccttcttatt tacatcaaat cgaggatgct
    21301 tcttcccaca aatggagagg acaaagagga agaagatccc cgggatgaac ttattgacag
    21361 aattcttgag tatgaaagac tccgggtaat ggttgattcc attgatggtc ttcccatgct
    21421 ggatagggat atcttcacga gaggaaaaac tgttgtggag gcggaaacag attttgacct
    21481 cctggccttg tgcagcatat tccttgaaat aatgaagagt aaagaggaaa aatacattga
    21541 aatcaaagag atacgaccaa caattgaaga aaaacttaaa atgttgaagg caatccttga
    21601 aacttcagga atttatgttt ggaatttcga tgaaacgatt gaacaaccag aaaaaatcgc
    21661 taccgtttta agcattcttg aactcaccaa ggttagaatg gcaaccattg tccaacgcag
    21721 accctttgga aaaatcatta ttagaaagcg agatgaccac ggcaacatac cctctcaacg
    21781 aataacaaac acttccattg ttggcaaatg agtataatct aaaaatcagt cttgatttat
    21841 caacatacaa ataagaaaga tgttgataag aataacatca tttgatatcc cataccatgc
    21901 ctgcatcgat tcatttcaat gatattttac tggtttttgt ggtcatctct tccatggcct
    21961 tagctgttat ttttccagac ttcggtttca gattcaggat gctaccctac tactgtctga
    22021 tgatcatttt ctttctcagt tacctttcca tcgacattgc ttcaatatgg accacactta
    22081 agagtcacag cactgaaatt actgtgtttt caataacaaa gctggttgtt ctgcctgttg
    22141 ttttttattt tgccttctat ttcatcgcac ccagttatgc cctttctgcc ctgcttctga
    22201 ctggagtttc agtaggtgtc gttgctccaa tgatttcaaa catggtacat ggcaacagtg
    22261 ctctggttct ggttatagtg gtaattacat ctgtggctgc tcctttcact ctgccagccg
    22321 ttgttaaaat aatagcttct aaggatgtga ccatctcctt tatgtccatg ctgcagatgt
    22381 tagctacggt tatttttgtt cccattatag ttgttgaaat cattcgccac ctcctgccaa
    22441 gacttgtgaa accgattctt aaaatcaggt tccctgcctc tcttgtgctg tttgctgtga
    22501 tcaatcttgg ggttttttac cgttacgcat ctttttttaa aaacgaacca tgggtaattt
    22561 taatgtctgc cattgtagtc tttgtcctgg cagcaatcta ctgtgcaatt ggcatagtat
    22621 ttttccgtaa atcgtcgttg gaggataaac tagcaggcgc agtcatgctc ggtaatctca
    22681 ataatgtact cataattgtt ttttcctctg aattttttgg tcctgtggag ccgctggtcg
    22741 ctgccatgta taccattcct ttctgggtga tcgtgatccc ccttcgttat tataggaatc
    22801 ggatgacacg agctatatag tcagttcaat aaaggccatc ttgaatgcag tttcttttca
    22861 acattccctt gcatcataaa atacagagca ttagtttctt ccagtcaaga ccgcggtact
    22921 aaaatataca tacggtatat gaatgaaaaa tgcactcacc cacgtttgaa agaagggttc
    22981 cctatatgtt gtttgcttct tcgagccact agtgacatta acacatgtgt tggaacctat
    23041 gcagccaatt ccatgcgctt aattggtaga cttgaacatt tttgcccatt gaatctataa
    23101 tatttgcgac cgttacgggt tgacaatact gggcattttc aaaagacaat atgaacaata
    23161 ccaacaacaa caacaacaac acagggtgga aagcaagcga agagactaag ggattcaacc
    23221 atactgacaa aaaacaatgt gcctcacaaa tttgaggcaa tctgctatac ttataggtta
    23281 gatatatatt caaaggagat gatgttaaat gttaatgcca accaaaattt tagtaccaac
    23341 ggatttttcc acttactctg ataaagctct ccgtcaggcc tttgatattg caaaacagta
    23401 taatgccaag gtttatgtga tccatgtggt ccatgaaaaa ataactgaca ctattgatga
    23461 ttatggaata acctatccat catatgtgaa agagattgaa gggaagatga tcgacgaggc
    23521 aaagaaaaaa atgaaggaac agatagataa atttcctcaa tcaaaagaac ttgagatcga
    23581 aagcgatgtt cagatcggca atatctctga agctatcctt caagaagaaa aaaacaaagg
    23641 gatagatctc atagtaattg cgtctctcgg cagatctggt attgcaaaat atctcatcgg
    23701 aagtgtggcc cggaatgtcc tgaaaggagc aaaatgccca gtcttgctga caaaatgaaa
    23761 gataatgcta tgattaagga gttatcatct gaaagattca cacacaacaa ctgaaaaata
    23821 tgtaagtgga agattcttta tgttttttca ttaaatgcat tgacaacacc gctccttgtc
    23881 ctcaatctgt agggaactgt tgaaataatc cttaactgaa gttgcgatag aatgggagca
    23941 ttgtagcctg gtgctggcta tgtgtgaatt atcagaagta agattcgtat gcaacaatac
    24001 tttattgtat ccagattagt tttcgataat atattataat gaaatggtat agacttaccc
    24061 tgtcttacga aatgagatgc ttatgaaata cgacacagtg gatgcgcgaa tatcaccgga
    24121 aggccgtttg aatgttttat caaaggtcga ggtaaatagg ctgctcgaca ccagtcacgg
    24181 tgggttgtac cggctgttta gaaattgctc gcttgcagtg cttaattccg gtaattacct
    24241 cgatgatggc aaagaacttc tggaacgcta tagatcattt gatatccgca ttattcagga
    24301 agagcgtggc ataaaattgg aagtcaagaa tgcaccggcc acagcctttg tggatggaag
    24361 gataataagg gggataaacg agcacctttt tgcagtcctc cgagacgtta tatacaccaa
    24421 cgatgaaata ggctgtaacc cgaaattaaa tttagaatct tcagccggta tcaccaatgc
    24481 agtattccat atcctgcgca acgccaatat ccttcgccca acacgaaatc cccgtttggt
    24541 tgtatgctgg ggcggacatt caatttcaca gttggagtat gactacagca aattggttgg
    24601 gtaccagttg ggcttgcgtg gccttgacgt ttgtactggg tgtggccatg gtgccatgaa
    24661 gggtcccatg aagggtgccg cagtcggaca tgcaaagcag cggattaacg acggtcatta
    24721 cctcggcgtc tctgagccag ggataatagc tgctgagcct cccaacccta ttgtgaacga
    24781 tcttgtaatc ctcccggaca tcgagaagcg cctggagagc ttcgtgcgga tagctcatgc
    24841 cgtggtggtc tttcccggag gtgcagggac tgcagaggaa atcttttacc ttctcggcat
    24901 cctcatgtgc tcagacaacg caaatatccc tttcccatta atatttaccg gccccaaaag
    24961 ctcaaaaggc tatttcaacc agatcaacca gttcatcggt catacattag gccctgaagc
    25021 ccagacccgt tacaagatta ttattgacaa tcctttggct gtcgctcgtg aagtccaggc
    25081 tgggataagg caggtgagag agttccgtaa gaatactgat gatgcatact acttcaactg
    25141 gcttctcaag attgacacgg aatttcaaaa accatttgtt gcgacccatg aaaatataaa
    25201 gaacctcgaa ttacacaaga atatggaaag gtctttactt gcctcgaacc tgcgccgcct
    25261 tttttcaagt gtagtatcgg gcaatgtgaa agatgaaggg attaggatcg ttgaacagca
    25321 cggtccattc gaaattcacg gagacatgga aattatagaa tctctggact ccctgttgac
    25381 ttcatttgtg gagcaacagc gtatgaggct tccgggaaca aaatatgtcc cctcttatcg
    25441 cattataaga aagtcttgac cgtcaagact gggtacggaa ggtgtacaat caccaataag
    25501 tcgtcgctct cagaaagcag cacatagcgt ggtgattcaa cgcaaacaaa aaggggacca
    25561 tggtcccctt tttgttcttc gtggttcgct tacttctttg ccggtgcagg ggcaggggcc
    25621 ggtgcagggg caggagccgg tgttgcggca ggcttcgcct cctctttttt ggcctcttcc
    25681 ttcttcggtt cagctttctt agcaggtttt tttaccttct tagctgcttt tttctctact
    25741 gctggttttt cagccttctc tacagccttc tctggagccg gagccttctc agcagccact
    25801 gccgctggtt tatcagccgg tttcgcctca ggttttgcct gtgcaaacac ggttgtgacg
    25861 aacgcaacgc tgataagcag tgcgagtact aacataagag ctttcttcat atcttttcac
    25921 ctccttcctt ggattagtta atcttcttta aacttgatat gcacatgaca tgccaaagaa
    25981 ataaagatat aaatactaat taaatcatta tgatagaaga tcgcaggaaa aagagaagaa
    26041 atccttagtc ggaattatcc gactaaggat ttaatacaat cggatatttc cgactctacg
    26101 gatacaataa attcttttta tttttattat gagaagttag cctgagcata tgcgctcaaa
    26161 agggctgttc agctccagaa ctaatttata caagtgtaac aaaaaatata agacgactat
    26221 aggtaaatgc taaataacaa aagatatacc ggttgcggta tgttttatcg ataatgcagt
    26281 gcaaactcta cagggaccga accagcaagt ttcttgacct ccgtttccac ctttttgcgc
    26341 tcatcaggat tgtggacaat accggtaacg agaagtctcc cttctccagg gataacgtca
    26401 aatactggta taaaaagggt tgggtgtgta acgatgccag ctctaacctt tttcgcaagg
    26461 gctttcatct ggagggcttc catcgctgtg tcggttttaa gtttatcctt ttccaaaagg
    26521 gcggctctta ttgtccccat catgtcatgg accggaataa cgcccacatc aaaaaaaaga
    26581 tcgtagctag atggttcatc aatatttttt ccatatataa aatggaccgc ccgtgacatc
    26641 tctttatcca ctttttttac aaaaagctct gcagcactcc tgctcaggtt ttccctcttc
    26701 atcacttcct caagcctttg ctcgattggc gccacaattc tgacattcaa tgcaaagggc
    26761 acttccctca gaaggaaatt tcctcccctt cccacaatga taacattgtc cttgagtgca
    26821 taatttaaaa tgatactctg gctcagtatc acaaaaccag cgaaagaccg gtcaaatctc
    26881 tcccagaggt ttgggtaatg ttcgtcgaat tccttgccaa acgcttccca ctgactgccc
    26941 tgagcactta ggtcttccag gatctttcgc ctatctacat attcataatc ccacaaacgc
    27001 gccatagttt gtggaatctc cctatcaata cttccgagtt gcctcgatat ggtcaagact
    27061 gccataactg gcgacctcct tatgttttgt gcagaccagg gattcttcct cctgttccct
    27121 ttttactttc attcttatcc agagtttccc taccagaact acggcaatag cgcctgcggc
    27181 ttccacaaca tactgggtga ttttgcctgt gtggagaaaa gactgcacgt atgggtcggt
    27241 aattatcatt tcacctgaga cctttcccag caccatggct ccaagataga caatgattgg
    27301 gtatttgtcc atcagggttg aaagtagatt gcttgtaaat acgataaagg ggatgctgag
    27361 gattagccca aatatgatca aaagattatt accatgagat gcccctgcaa cagcaagcat
    27421 attatctaaa ctcatgacaa tatctgcaac aactatcgtc accatagcct gccagatggt
    27481 ttttggctct ttcttggctc cttcatccgg tgcaccttca atgaaaagct ttaatgctat
    27541 ccacataata agtgcaccgc cgcagaactt caatcctttt atgccgagta gctgggcaac
    27601 aaggattgtg agggctaccc tcaatataat tgcaccgcct gcacctaagg caataccgag
    27661 cgacctttgt ttccttggaa ggtttcgtac agccattgcg ataacaacag cattatcccc
    27721 tgccaggaca aggtcaataa gaacaaccat taagaaaccg cttatgaact cccatgtaaa
    27781 attaaaatat ccaaagcttc caaaattcat aatatttacc ccttcaccaa ttttgtattt
    27841 aaactattcc aacgtccatc ctgtttggta tttccacata aatactctat attttattta
    27901 acctggatgc ttcttaccgt ccatcagggc aaatctgatt tttgacttct gaaaacctga
    27961 tatcagggtt atcatcagga tgagggttca caactctaca attcttaatc acattggcct
    28021 ggcatgctcc aataattgaa cattaaacgg attatcaggt tggggcatca aatccacgta
    28081 tagtcactgt tctggtaagc acaccgggcc cggtactacc tttagaagta aaaaatcagg
    28141 ttattcccta tgttcaggta ttttctgaaa tgatattttc ataagagcaa attggagtca
    28201 tgatcaaacc tggagcaagt acaaactgcc aaaacataca acttcttgct gtcctattca
    28261 tatctccata gtagctaatg gcacttcact ccctccgggc gttttttcac atcccccgaa
    28321 accccctata atgctattga aacgataaat cgcccggagg ccccattctt aacatttgct
    28381 ttagaattgc cgataatata aataccaaac gcctcctttc ctccgggtat ccaccatatc
    28441 ctgcccacat gtaaaaccat tatacccgga ggtttgctgc aacgatctta taacaactaa
    28501 agatacactc tttgggtata ggtgaatgtt tctcaacctt taaagtaaac gcactcgctt
    28561 tctgccatag gctcatctga agggtgtacc tgcaaaatcg gtcgatctgt gtgctatttt
    28621 ttttatgagg aaaaggtaaa tttcccaagc aaatacttca ggaaatcgta tctctccctg
    28681 gcaggaaggg ctttgtatag gtttgttgca atcaatatgc ggataacttt tttaatctcg
    28741 ctttttctca caacaaaacc ttctgtatca gggtaagctg gacaggcatc ttcgtatctc
    28801 atatctgaga taattatcgg gcatacttct gtttttcata ttgacagtta tcacatacat
    28861 ggtatctgtc gtaagaacga gacaagacag tttgcgtttg tgaaccatgt tcttaaaagt
    28921 atttaaactt cgcaaaatca tggagcctct tagacggtct atagcaacag ctatactgct
    28981 ctgcacccta tctatccatg ttcatggtaa caatatgctt gagtgatgct ctgtt
//
LOCUS       DAOOMC010000021        28242 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdus sp. isolate AMR_MDS_2685
            NODE_1474_length_28242_cov_6.226594, whole genome shotgun sequence.
ACCESSION   DAOOMC010000021 DAOOMC010000000
VERSION     DAOOMC010000021.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864760
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdus sp. (wastewater metagenome)
  ORGANISM  Syntrophorhabdus sp.
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae; Syntrophorhabdus.
REFERENCE   1  (bases 1 to 28242)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 28242)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,729
            Genes (coding)                    :: 2,708
            CDSs (with protein)               :: 2,708
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 7 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28242
                     /organism="Syntrophorhabdus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1474_length_28242_cov_6.226594"
                     /isolate="AMR_MDS_2685"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2699756"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(121..528)
                     /gene="rnk"
                     /locus_tag="PK125_03560"
     CDS             complement(121..528)
                     /gene="rnk"
                     /locus_tag="PK125_03560"
                     /inference="COORDINATES: protein motif:HMM:NF004396.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside diphosphate kinase regulator"
                     /protein_id="HPB37218.1"
                     /translation="MRKREIFVTQRDMERLQALIEIGGRQDAAYLESLEEELDQAKVV
                     DPKDIPNDVVTMNSTVLIKDLDSNEEKTLVLVFPGKTNINENAISILAPLGTALIGYR
                     EGDIIDWEVPSGKKRIQVIKVVYQPERAGDYQV"
     gene            complement(854..1732)
                     /locus_tag="PK125_03565"
     CDS             complement(854..1732)
                     /locus_tag="PK125_03565"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HPB37219.1"
                     /translation="MTHDVRILHAADLHGNLTHYRELLSLAVAKQADCVVMGGDLLPG
                     GISISSLAEVQRRFLNDHLRPLLEHFKETNPEKTVYLMMGNDDLAINMDQLERMAGEG
                     LLRLLHLRMYTLSDSLFIAGYGCVPPTAFMIKDWERLDAHRSMVQDRSYQAYSSGSEG
                     INVVNTHEWFLSHNTIEEELSMLARLSNPAHTVYVSHAPPYATKLDVLFNGRHIGSRS
                     VRMFIEQHKPPLTLHGHIHESHHMTDEIKDCLGPTVCINPGQSRQVLHAVTIDMAGNE
                     VHRIVSYPRKETVFSR"
     gene            complement(1751..4972)
                     /locus_tag="PK125_03570"
     CDS             complement(1751..4972)
                     /locus_tag="PK125_03570"
                     /inference="COORDINATES: protein motif:HMM:NF013491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HPB37220.1"
                     /translation="MEDSSDPKGASLDTLKERAKELDCLYQVDEILGNSGLTLTEMFE
                     RIVLVLPSGFRFPEVCRVEIAYDNHRYQSADFARSRISDGVDIKAGGKVVGRITVTYI
                     DEVSKTDEGYFLEKEKRLIKTIADRIEQTIFFRRAERLIEEWDIPEKDGLPKHPTAAD
                     WMAIIALLQKTDQDMLLHICRKMVNHLCYRGIREAEQELCKLNFGSKVFPVAGEVNFP
                     SQMLPLGDLAKISEKAFSIASKHMTDGEISTRLMKWIQETKAYTLIKTIDRIDTSIGD
                     VVEAVSRYTNTAGTVNMLDPPTERWLRVALIRRFLSDKPGFIRIAQQYLDIRGFYDIV
                     QTLIYPAGSHGKIGGKATGLFLAKEVLKRESERISLLDEIKIPKTWYIATDEMREFLH
                     YNNLQGLNEQRYRELDEIRIDYPNIIQMMKNSQFPPHVVKSLAMALDDMGNSPLIVRS
                     SSLLEDQSGAAFSGKYKSLFLANQGAKKDCLEDLKNAIIEIYASIYSPDSIQYRAERG
                     LLDYPEEMGIMIQEVVGRKIGPYFLPCYAGVALSNNEFRWSPRIKRSDGIIRIVPGMG
                     TRAVDRVGDDFPVLVSPGQPGLRVNTAPEEIKRYSPSKIDVINLQKNTFETIDALSFV
                     REFGHLVPDIQNIVSVFRNDYISTPPAFDLDFEHDHLILTFDGLISNTSFIKKIRAIL
                     NVLEEKLETPVDIEFASDGQDFYLLQCRPQGFREDSSPSAIPKDIDWRHILFSAKRYV
                     SNGSLKDISHIVYVDPEAYDGLMKIEDLEDVGRSIGLLNSMLPKRRFILMGPGRWGSR
                     GDIKLGVKVSYSDISNTAALIEIARAKSNYVPELSFGTHFFQDLVEANIRYLPLYPDE
                     AGIVFNEVFLRKATSLFSKILPDYSHLEDVVRVIDVADSMEDNVLHVAMNAELEEALG
                     YFGPHSGDKSLPFQPMVGMKRIQKDTQVRFDDVFWRWRSYMAERIAGRLDPSRFGVKG
                     IYLFGSTNSGTAGPGSDIDLIIHFDGTDEQRKDLLQWLEGWSFCLAEINYLKTGYSTD
                     GLLDIHIVTDEDIAKKTSYAVKIGAITDPAHSLKLKKNPKDPI"
     gene            complement(5003..6289)
                     /locus_tag="PK125_03575"
     CDS             complement(5003..6289)
                     /locus_tag="PK125_03575"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HPB37221.1"
                     /translation="MEKSSYNPFTNAQAQFDKVASFIGLDEATCDLLRQPLKEHHVLI
                     PVCMDDGKMKIFKGFRVLHNDARGPAKGGIRFHPQETADTVRALAMWMTWKCAVVDIP
                     LGGGKGGVICDPHNLSEREQERLCRGWVRQLSKEMGPYIDAPAPDVMTTAKHMLWMLD
                     EFETIHGGRYPGFITGKPVGMGGSTGRTEATGFGAIYVLREALKDLQIKLEETSLSVQ
                     GFGNVAQYAAKMFTELGGKVVAVACWDNEDKVSYTYQKKEGLSMNMLLEITDSFGCIN
                     KKKAAAAGCKILPGEEWIEQDVDILMPAALENQITAKNVNKISKQVKVLLEGANGPTT
                     TDADDIIKERGIFLLPDFLANAGGVTCSYFEQVQSNLNYYWEKEEVLEKLDTKMTSAY
                     RAVYDLAKKKNLYMRDAAYVIAINRVAQAVKMRGWA"
     gene            complement(6434..6784)
                     /locus_tag="PK125_03580"
     CDS             complement(6434..6784)
                     /locus_tag="PK125_03580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB37222.1"
                     /translation="MKNFSARKKKGDTLPVRGKDRMDLCTFCFAAPYCVLVNSSAAWA
                     LECEEFRAYDPTQESGMCEKNSLEAKSDEGSQYMPVRPEYLKNRGLCSDCAIYDSCPF
                     PKNRGEVWCCEEYR"
     gene            complement(7024..8382)
                     /locus_tag="PK125_03585"
     CDS             complement(7024..8382)
                     /locus_tag="PK125_03585"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HPB37223.1"
                     /translation="MVRCTDDKDRILIVDDSAATLEVLHRNLVSAGYMVFAALNVTEA
                     IKILDVTPIDLVITDLKMPGLSGVHLLRHIRENFSDMEVIIITGYPSVKGAVEAIKTG
                     AEEYLAKPFTDEELFSAVASTLRKLHTRRAASRFPTKKITTSYGLIGESPAMAGVRDF
                     IAKAALTSATVLIAGESGTGKELVARAVHYSSNRASAPFVPVNCGAIPEELLESELFG
                     HMKGAFTGAIDSRAGFFQTADGGTIFLDEISETSLNMQIKLLRVLQDKEVCMVGSSRT
                     RKVDVRIVASTNKDLYALVKKGLFRQDLFFRVNVLAIELPPLRSRGDDILMLIRHFSE
                     RLAEEMGKPVPAFSSQTLQVLQNYHWPGNVRELQNVLQRLIVLTDGDIVETPDLPQLL
                     RFSVCKEEDFTRTLADVEARYIRNVLASVKGNRTRAAAVLGIDRKTLRKKLQKSSQSV
                     KV"
     gene            complement(8387..9526)
                     /locus_tag="PK125_03590"
     CDS             complement(8387..9526)
                     /locus_tag="PK125_03590"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPB37224.1"
                     /translation="MKACANLNEQTKLNLLERVKELSCLYRIAHLAANAGTALGKVLE
                     GITGILPSAWLYPDIACARIVLDGHSRSAKEYPKRVRHRQRTPIVIRNKRRGYVEISY
                     VEEKPERDEGPFLNEERALLDAVANEIAVIIMRREAEQEKLNLEEQLRHADRLATIGQ
                     LAAGVAHELNEPLNHILGFAQLIKKNPGLPDQVQADVDKIVNSSFHAREIVRKLLVFS
                     RQVSPQKTKVDLNYIVKNAIGLFESKCASDGIEFQCALQPDLPKIDADPSQLHQVFVN
                     LIVNAIQAMSKGGKITISTLKDQAKACLLVEDTGAGMGKNVLQKIFTPFFTTKDIGTG
                     TGLGLPVVHGIVTAHGGSIKVASKVGRGTKCRIELPLRKEDHPDR"
     gene            9707..10960
                     /locus_tag="PK125_03595"
     CDS             9707..10960
                     /locus_tag="PK125_03595"
                     /inference="COORDINATES: protein motif:HMM:NF023428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HPB37225.1"
                     /translation="MRPKNLDEVVGQEHLLGKGKILRVLLDKRDVPSLIFWGPSGVGK
                     TTLGWLIGKHMKLPYVSLSAVNIGIKEVKEIIQKAKHQKIIIFIDEFHRFNKLQQDTF
                     LPHVENGDIVLIGATTENPSFEIISPLLSRMKVLTLKALEPKDLTTILRRALNEDHEL
                     KNATLRIDEATIEELALLANGDARKALNLLEVCFKMAKKEGKEESVIDRNVLREAYQK
                     NTAVYDKKGEMHYDLISAFHKSLRGSDADASLYWLARMIEGGEDPLFIMRRMVRFASE
                     DVGNADPQALMLTMAAMEAFNFIGPPEGYLAIAQACVYLALCEKSNAIYVAYGRASSD
                     ARELPEYPVPLHIRNAPTKLMKELGYGENYLYPHNFENGLVSQEYFPPEIKEKHYYFP
                     SNRGYEQKLLAFMDKVRKVKGKDSL"
     gene            11018..11983
                     /gene="thiL"
                     /locus_tag="PK125_03600"
     CDS             11018..11983
                     /gene="thiL"
                     /locus_tag="PK125_03600"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR01379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HPB37226.1"
                     /translation="MEIPENRLIDKIRRFADRSRDVLRGIGDDGAVIKLSQGSYVLVQ
                     DAMVEHVHFKFSFTNPYDVGKKAVYVNISDVLSMGALPLYFLVTIGITPALTSKDIGR
                     LYHGMRQAAREFGAIMIGGDTTESKSDFFIDVSMIGKLVTKTYLGRDRAKEGDLIAVT
                     GSLGESAYGLHLLLEGKPTKGMSTFLKRYASPKPPYPVWKEIVKHDITRAMMDISDGL
                     LIDLGRMMAESKKGAVIHLEKLPIPPLLRKKNKEVLALSGGEDYQLLFTFPKSRLKKI
                     QGMIQQGYPISIIGEVKQGKDVTVLSQGKVYEAPEKGYEHFGNSR"
     gene            11980..12951
                     /locus_tag="PK125_03605"
     CDS             11980..12951
                     /locus_tag="PK125_03605"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HPB37227.1"
                     /translation="MKKQIFVKDITKEYMDINDFFVVTKKGTFLRKNNIKYMTIGLRD
                     TTGVIEGKIWERVDEFQDMFDKNDLVYVKSKSRFYQDKPQLNITDIKRIEEPLTMDEL
                     KAFFPEGQRASKTVREEFAELVNSIQSPYIKSLFSAFTEQSGLADKFYLYPASIGVHH
                     VYLGGLLEHSVSVAQMAKEAVRFCGGNLDIVIAGSLLHDIGKVDEMEIKGTFRYSDNG
                     RLLGHITLGVIIFDSLVKKIRDFPIQISDIIKHIIISHHGVEEWGSPRKPMTIEALIV
                     HYIDNLDAKVAGVKEYMMENMEDGTWTTFHRLYESRFYKTQEGVYGD"
     gene            12941..13678
                     /locus_tag="PK125_03610"
     CDS             12941..13678
                     /locus_tag="PK125_03610"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmE family RNA methyltransferase"
                     /protein_id="HPB37228.1"
                     /translation="MEIRRVFVDKLKIKNNLVLITGPMQNYISSVLRKNTGDRIDIID
                     GKGYLYRCTVNSIKSKEVFLQILDVVHRPEEKRPKVTLCVSPIKGPRMDWLIEKATEL
                     GADRILPTIFKRTLIRIEDKEKEKLERWRRLAIEASRQSGRFTIPEIIPPTPLRGITT
                     HLNGVQNKWVLYEKEKKTSIKEVISSQRENGICIVIGPEGGLEESEISWLTENGFISC
                     TLGESIFRAETTPLVVLSIILYEYSKK"
     gene            13675..14103
                     /locus_tag="PK125_03615"
     CDS             13675..14103
                     /locus_tag="PK125_03615"
                     /inference="COORDINATES: protein motif:HMM:NF018028.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HPB37229.1"
                     /translation="MRKASILFRFFAFVVDILVLFFFISLLFAAIVTGYLLSSGSILI
                     PQISLLTFIFACGSLFTFIFYFTYLTMNGGATVGKRFFQLRVLRQDGSGLNAGRAFMR
                     TMIYPLSLTLWFVNVLSALLLDGRMIHDIVVGSWVVRVEL"
     gene            14100..14756
                     /locus_tag="PK125_03620"
     CDS             14100..14756
                     /locus_tag="PK125_03620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB37230.1"
                     /translation="MNHRGMAGCGCLIVILIIIMLFTGALMHPITLRFLGTQFHYEDR
                     IFTSDAIFVPRFPEDRNGELYAASFQDFSAGNGKAILVEDDTVLGTSIVELVNKMAKA
                     RNVKDDGIKRISASGEGLVKAQRVKEQIEKQGLRKIIIIVPDYASRRFHLMYDSARAG
                     GKTLYLIKPVPVTYFNRDKWWKDSGSRGILAEEIFSISHYYLQRFKYGEKEMKDREKR
                     "
     gene            14985..16037
                     /gene="dprA"
                     /locus_tag="PK125_03625"
     CDS             14985..16037
                     /gene="dprA"
                     /locus_tag="PK125_03625"
                     /inference="COORDINATES: protein motif:HMM:TIGR00732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HPB37231.1"
                     /translation="MDDKVAIIALHRVEGIGSAIKRRLVDMHENLAPLFLGKVKLGDD
                     RVYKKIVAFKGWKDIDRDMKKISDMGAEILTIKDREYPDQLRNIPDAPIVIYKKGRLK
                     IGADTIAIVGSRAASFESINMAEKIAQTLSSSGITVISGLARGVDAAAHKGALKEKGK
                     TVGVLGCGIDICYPAENRPLFKKIEEDGCILSEYGLGEPPLPHHFPMRNRIIAGLSKG
                     VLVVEASQRSGSLITARLGLEYGREVMAIPGNIFDEGYRGTNSLIKQGAKLIDGIEDI
                     ITACFSGLTFKTDKHVDLDNNERYIYGIIGSTKIHVDEVVEKSGMETKHVMAILTNLE
                     LKEAIKGIPGGFYLRI"
     gene            16090..18297
                     /gene="topA"
                     /locus_tag="PK125_03630"
     CDS             16090..18297
                     /gene="topA"
                     /locus_tag="PK125_03630"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR01051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HPB37232.1"
                     /translation="MGKSLLVVESPTKMKTLSKFLGKDYVIKATYGHIKDLPKSKLGV
                     DIEKGFTPQFLTVKGKTKVVDELKKVGKDAEKILIGSDPDREGEAIAFHVADVVGKKK
                     EVKRVLFHEITKKGVLDALKNPTTLDESKYDAQKARRILDRLVGYKISPLLWERVSYG
                     LSAGRVQSVALRLVCDREEEIEAFVKEEYWVIDVELQLNSGEIFTATLEKKEGEKIKI
                     TSKDEAEAIKKAIEGKTLLIDKIEIRDKHVYPQPPFITSRLQQEASRKLKFSPKRTMI
                     LAQKLYEGVDIGEEGVTGLITYMRTDSVRVSNEAVKDARKFIVNSFGEPYLPKIPHFF
                     RNRKTAQDAHEAIRPTYVELSPDKIKSHMDKDMLALYELIWKRFIASQMAQEKIKTKT
                     VEIEGGDYTFVAHGSEVIFDGFTKIYEEEKEDEEEGTYLPDMKQGEEVFLKDTILNQR
                     FTMPPPRFSEASLIKTLETKGIGRPSTFATIVSTVQERAYVKKDKGRLFPTPLGRTVN
                     KLLKQFFPLILDIDFTAKMEDRLDLIEVGKKNWIQSLEKFYELFDKDLSAAQEGMKSL
                     KKEETETDIICDKCGKTMLLRWGKNGEYLVCSGKPTCKNKKNVKVEDNGTIRIVEAEI
                     MGTCGQCGGNLIEKRGRFGRFIACSNYPECKYTEPFNIGFQCPEEGCTGKLIEKISKK
                     KKKFISCSRYPNCTFATNTEPTEGPCPQCGAPTLFSSRKKTRCLRKDCGWTLQ"
     gene            18282..19577
                     /gene="trmFO"
                     /locus_tag="PK125_03635"
     CDS             18282..19577
                     /gene="trmFO"
                     /locus_tag="PK125_03635"
                     /inference="COORDINATES: protein motif:HMM:NF003739.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate--tRNA-(uracil(54)-
                     C(5))-methyltransferase (FADH(2)-oxidizing) TrmFO"
                     /protein_id="HPB37233.1"
                     /translation="MDITVVGGGLAGVEAAYQLASRGHSVSLFEMRPTRYSPAHKTPL
                     LSELVCSNSLKSKELTNAHGLLKEELRILGSIVINTADQAAIPGGKALVVDREIFAKR
                     ITREIEANPLITVIRREIQDIPAGTVIIASGPLTSNALAKRLAEITGVNNLFFFDAIS
                     PIIDGGTVDMDKAFFGSRYDENSDDYLNCPMTGEEYDAFYNALIGAEKVDLQPFEKTS
                     FFEGCLPVEIMAQRGRQTLAYGPMKPVGLKDKTAGKEPYAVLQLRRENMAGTMYNMVG
                     FQTKLTYAEQDRVFRMIPALRNAVFLRYGSIHRNTYINSPALLNNSLCLKGQDQTFFA
                     GQITGVEGYVESVAMGLVAGISAHVSIMNQVFSPPPEDTCVGALLNYITTPNDNFQPM
                     NINFGLIKNYRKRAKEKVIHNALQAISLWNEEIDEISKK"
     gene            19620..20600
                     /gene="bioB"
                     /locus_tag="PK125_03640"
     CDS             19620..20600
                     /gene="bioB"
                     /locus_tag="PK125_03640"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00433.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004076 - biotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HPB37234.1"
                     /translation="MESKIKRLATKAINKKGISEDDAKSLYEIGKDYPFMVFAYGSEI
                     REHFKGKKIDLCCIINAKSGICPENCRFCAQSAHYHTTAPVYPLLSAAEIIKKAQEAK
                     ESGAHFFSIVTSGTAIGNKEEWNEIYEAIKGIAALGLKPCGSLGMLTDEQASALKNSG
                     LYRYHHNLETSRTYFKKICSTHDYQDDIETNMAAKRAGLSVCCGGIIGLGETMDHRIE
                     MAITLKEIGVDSVPINILNPIPGTPLEGNPPLSPFEILLTVAIYRFILPDKDIRLCGG
                     KEKNLRQLLPLAIIAGCNSLMTGNYLTTLGRETAQDVEMIHDLGLRATYG"
     gene            complement(20707..21915)
                     /locus_tag="PK125_03645"
     CDS             complement(20707..21915)
                     /locus_tag="PK125_03645"
                     /inference="COORDINATES: protein motif:HMM:NF012694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HPB37235.1"
                     /translation="MPLYEWQGKTGRGEARSGTLKADSEAILRTTLRKEGVIVTKITE
                     KKGGEKAVKVNTNKKIKPLAVVLFTRQLSTMITSGLPLVQSLEILSNQIEDKNLCGIV
                     KEIKAKIEGGSRFADALRDYPKCFDELYVNLVVAGEEGGMLDTVLNRLAIYMEKIEKL
                     KKKIKSAMIYPISIIVVAIAVVMVLLIFVIPVFEGMFKDMGAELPLPTQVVINISKIV
                     KSYIIYMIIALAIGVFLFRRYYKTESGQRTIDRLVLKAPVFGVLALKSSVARVTRTLA
                     TLLSSGVAILESLVIVARVAKNKIVEDALIVARAKISEGKSMSEPLQDSGIFPPMVVQ
                     MVQVGESTGALDNMLNKIADFYEEDVDNLVTNLTSLMEPLIMMFLGVVLGGLIIAMYL
                     PIFKLGQAVG"
     gene            complement(22046..23506)
                     /locus_tag="PK125_03650"
     CDS             complement(22046..23506)
                     /locus_tag="PK125_03650"
                     /inference="COORDINATES: protein motif:HMM:TIGR02037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegQ family serine endoprotease"
                     /protein_id="HPB37236.1"
                     /translation="MKNKRWYFTIFALIIVVLAFGYLRGKVNTGDTWFDANQSNVKNV
                     VFDKGLPSFSGLVKSVKPSVVNISTTSVIKGPNMHDRFFGQQNPFKDFFGEEFFERFF
                     GDSQKREFKQRSLGSGCIIDREGYIITNNHVVEKAQTIRVKLIDGKEYDATIVGRDPK
                     TDLALIKIDARNSLPAASFGNSDNLEIGDWVVAIGNPFGLETTVTAGIVSAKGRVIGA
                     GPYDDFIQTDASINPGNSGGPLFNLNGEVVGINTAIVSGGQGIGFAIPINMAKELLPQ
                     LKSKGKVTRGWLGVVIQKITPEIARTFGVKESEGALVSDVTEGGPADKAGIKRGDVIV
                     SFNGKDIKDVDTLPKLVGGTEVGKKAKVGIIREGKSLAVDVVIGELKDEKIQASKKPE
                     VEKDFGLVVQNITPEIAKHLNIKDRRGVIVTDIQPGSPAQEGDIKSGDIIKEINRKPI
                     KNIEDFKEAMKTVNIKEGAVILLQRDNTTFFTVLRG"
     gene            complement(23520..24923)
                     /locus_tag="PK125_03655"
     CDS             complement(23520..24923)
                     /locus_tag="PK125_03655"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal sensor histidine kinase"
                     /protein_id="HPB37237.1"
                     /translation="MMKKVNLPIKWRLTIWYGAVLSLILVGFASGIFIYFKNSLQESI
                     DTKIRSIGEVLSSSMTDSHNTSVFGNFERYLENVLGKKPKGKFIQIMDSSGRIGAKMS
                     DIEGEALPTSFSTLEKAMRGEVVYETTDRTKPRLRMITIPIIENKKVTSVVQVGTSLE
                     EFDETIKKLLLIMIISIPSSICVTIVVGYFMAKKALRPVDQIRRAAVKISSSNLDERI
                     DVSGRRDELGRLAETFNAMIDRLKDTFQRINQFSIDVSHELKTPLTILKGETEVALRK
                     EREKQDYQVLLKSNLEEIDRMSCIIDDLLLLSKADTRKIKLDVEEVSLRDLILDVCMD
                     MKVVADKKNVELEANELEDIRLKGDELKLRRMLLNIVENAIKYSHAGGRVRVSSHANN
                     GYANIYVKDNGIGISEMDIKYIFDRFYRADRSRKRASGSGLGLSISRWIAEAHQGGIE
                     VESQPAQGSMFTIKLPI"
     gene            complement(24907..25587)
                     /locus_tag="PK125_03660"
     CDS             complement(24907..25587)
                     /locus_tag="PK125_03660"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HPB37238.1"
                     /translation="MKLLLVEDEEKVAHFISKGLEEEGYTIDIAYDGRRGLELLKEST
                     YDLLLLDLMIPEIDGLDLLKTIRGWGNNTPVLIITAKSSKEDVVKGLDTGSDDYLTKP
                     FSFDELLARIRALLRRSKISDSHIIDYKSIILNPYTRKLHIETKAVELTEKEFLIMEF
                     LLKNSEKPLTRKEIADYVWQSQTDSTNIVDVYVNFLRKKIESVTSKRYIHTVRGTGYV
                     LKEDDEKG"
     gene            25834..27384
                     /locus_tag="PK125_03665"
     CDS             25834..27384
                     /locus_tag="PK125_03665"
                     /inference="COORDINATES: protein motif:HMM:NF037959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HPB37239.1"
                     /translation="MNLLEAYIVMFIASFCTLVIEMVAGRILAPFVGVSIYTWTSIIG
                     VILAGISIGAYIGGKLIDKFPVRKTLGWLLLLSGISALTIIPLTNLIAAYRFNMPLMM
                     RIFLVTAVIFFLPGCILGTVSPVVVRLTLKNLENAGNVIGKIYAVSTLGAIIGTFATG
                     FLFISWMGTRSIILTMAIILILLALFCGALFKTKKALAAFIVIAALCIWGVYAYAYKI
                     PSRENMYLYKESDYYTIKLLTTTSSDQKTPLQAMVLDNLIHSYVCLENPFHIEYEYER
                     IYSDVLKWKFRKNEDFKSLTIGGGGYTFPRYMELFYPNAQIDVVEIDPEVTKIVYNHL
                     GLSANTRIKTYNTDGRWFVMNCPEKYDLIFTDAYNDLSIPYHLTTKEFAMQLKSIMKP
                     DALLMSNIIDNFQVGAFLPSYIRTLREVFGHNNVYLLSVSPDFENTRISTFIVIAGNG
                     KLDIKDFDAHVKSYQQKHATSVIVPDNLMEEFMTKRSSIIIRDDYAPVDNLIAPIFEE
                     RFGYNRKS"
     gene            27473..27548
                     /locus_tag="PK125_03670"
     tRNA            27473..27548
                     /locus_tag="PK125_03670"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:27506..27508,aa:Gly,seq:ccc)
     gene            complement(27717..28031)
                     /locus_tag="PK125_03675"
     CDS             complement(27717..28031)
                     /locus_tag="PK125_03675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB37240.1"
                     /translation="MTYSLEDLNKKSDEELLRIKNGKMPHFMSAKSTTTPADLMAEAD
                     VILHKRQKEGEKRMSKMTLWILILTIVIAVLTAVLVFSEFKKTYSVDSSPKKDSYPEK
                     PK"
BASE COUNT         7904 a         6399 c         5955 g         7984 t
ORIGIN      
        1 ttactttccc agggatggtt ctatgcttgt taatttttag acccatatgt ggactacgta
       61 acatacatat tttcttgctt cttctttcat gaatgttaag tcatggatag gaagaatctt
      121 ttatacctga taatcgccgg cccgttccgg ttgataaaca accttgatga cctgtattct
      181 cttttttcct gatggaacct cccagtcaat tatgtcccct tccctatatc caatcagggc
      241 tgttcccagg ggtgctaata ttgaaatggc attttcgtta atattcgtct tgccaggaaa
      301 tacgagaacc aatgtttttt cctcatttga atctaagtct ttgattagta ccgtagaatt
      361 catcgtgaca acatcatttg ggatgtcttt cggatcaacc accttagcct ggtcgagttc
      421 ttcttccagg ctttccaagt aggccgcatc ttgtctacca ccaatttcga tcagcgcctg
      481 gagcctttcc atatcgcgtt gggttacgaa aatttctctt tttctcataa tgtttgttac
      541 ctcttagagt tttttatgag cacagaatcc ctggtagtaa catccctgca atcattgaga
      601 ttcttcacgt atctcggttt cacacaatta aaaatgctct aagtatggat gacatattat
      661 attaccatat ttcatagatt tagcccagtg ttcccgacac ttaagcatca attctttttt
      721 ttgagtacac caaaccatga aaatgtatat cctaatcagg catgtttggg tcccgtttga
      781 tcataaatgg cgggtttaca cagcagacaa cagtgaaatt gctaatttct ggggtcgaaa
      841 ctgtaaaaag agactagcgt gagaagacag tctcttttct tggataactt acgattctgt
      901 gtacttcatt tccagccata tcaatagtga cagcatgaag tacctgtctg gactgtccgg
      961 gattgatgca aacggtgggg cctaaacaat ccttgatttc gtctgtcatg tgatgtgatt
     1021 catgtatgtg accgtgaagc gtaagggggg gcttatgctg ttcgatgaac atcctgacag
     1081 accggctccc tatgtgacgg ccgttgaaaa gtacatccag tttggtagca taaggaggtg
     1141 catgactcac atagactgta tgagcaggat tggacaggcg tgcgagcatg ctaagttcct
     1201 cctcgattgt gttatgggag agaaaccatt catgggtatt gacaacatta atgccctctg
     1261 atccgctact ataagcctga tatgaacggt cctgaaccat tgaacgatgg gcatcaagtc
     1321 gttcccagtc ttttatcatg aaagcggtcg ggggaacaca gccatatcct gctataaata
     1381 gactatccga gagggtatac atgcggaggt ggagcaatct caggagtcct tcacctgcca
     1441 tcctttcgag ctgatccata ttgatcgcaa gatcatcatt acccatcata agatagactg
     1501 tcttctctgg gttggtttct ttaaagtgtt caaggagtgg cctgagatga tcattaagga
     1561 accgtctctg tacctcagca agagaggaaa tagaaattcc tcctggtaag agatccccgc
     1621 ccatcacaac acagtctgcc tgttttgcta cagcaagaga gagaagttcc cggtagtggg
     1681 taaggttacc gtggagatct gcagcatgga gtattcttac gtcatgggtc atccatctta
     1741 tgtccttctg ttatattgga tcttttggat ttttcttgag cttcaatgaa tgtgccggat
     1801 cagtaatagc gcctattttc actgcatatg atgttttctt agcaatatct tcatctgtaa
     1861 caatgtgtat gtcaaggaga ccgtcagtgg aataacctgt tttgagatag ttgatttcag
     1921 ccaagcaaaa gctccaaccc tccaaccact gcagaagatc cttccgctgt tcatcggtgc
     1981 cgtcaaaatg tatgatcagg tctatatcgc tccctggacc ggctgtaccg ctattcgtgc
     2041 ttccaaacag atagattccc ttgacaccaa aacgggaggg gtccagtctg ccagcgatac
     2101 gctcggccat gtaagaacgc caacgccaga atacatcatc gaatcggacc tgtgtatctt
     2161 tctgtatacg tttcatgccc accatgggtt gaaaaggaag ggatttatct cccgaatgag
     2221 ggccgaagta gcccaatgcc tcttccaact ccgcattcat agcaacatgg agcacattat
     2281 cttccatgga gtccgccacg tctataactc ttactacatc ttccagatga ctataatcag
     2341 gaaggatttt tgagaacagg cttgttgctt ttcgcaagaa tacttcatta aacactatcc
     2401 ctgcttcatc gggataaaga ggaagataac gaatatttgc ctccaccaga tcttgaaaaa
     2461 aatgagtccc aaaggaaagt tcaggcacat aatttgattt tgcacgagcg atttcgataa
     2521 gagctgctgt attgcttatg tcagaataac tcactttaac cccaagtttt atgtcaccac
     2581 ggctccccca cctgcctggt cccataagaa taaagcggcg ttttggcaac atggagttga
     2641 gcaagccaat cgatctacca acatcttcga ggtcttcgat tttcatcaga ccatcatagg
     2701 cctcagggtc cacatacaca atgtgagaga tatcctttaa gcttccattt gacacatacc
     2761 gtttggcaga aaacaagata tgcctccagt caatatcttt aggtattgct gaagggctgc
     2821 tgtcttcccg aaaaccctgc ggtcggcatt gcagtaaata gaaatcttgt ccgtcagagg
     2881 caaattcaat gtccaccggc gtctcaagtt tttcctccaa tacattcaat attgctctta
     2941 tcttttttat aaaagaagtg ttcgaaatca gcccatcaaa ggtcaggatt aaatgatcat
     3001 gctcaaaatc taaatcaaag gcgggcggag tgctgatata atcattcctg aatacagaaa
     3061 cgatattctg gatatcaggc accaaatggc cgaactccct tacaaatgat aaggcatcta
     3121 tggtttcgaa ggtgttcttt tggaggttta ttacatcgat ttttgaaggg gaatatctct
     3181 tgatttcctc aggggccgta ttgacgcgca gtcctggttg gccaggagac acaagaacag
     3241 ggaaatcgtc acccacccga tcgactgccc gcgtgcccat gccaggtaca atgcgaatga
     3301 tcccatcact tcgcttgatc cttggggacc agcgaaattc attgttactc agcgcaacac
     3361 cggcgtagca aggcagaaaa taaggtccta tcttcctccc aacaacctcc tgaatcatga
     3421 ttcccatttc ctctggatag tccagcagac ccctttctgc ccggtattga atagagtcag
     3481 ggctgtaaat ggaagcataa atttcaataa tggcattttt cagatcttcc agacaatcct
     3541 tttttgctcc ttgattggcg agaaacagac ttttgtattt ccctgaaaag gcagctccgc
     3601 tctgatcctc cagaagactg gaacttctta caataagagg gctattcccc atatcgtcca
     3661 aagccatggc caggctcttg acaacatgag gagggaactg tgagttcttc atcatctgaa
     3721 taatattggg atagtctatc cggatctcat cgagttcgcg gtacctctgt tcattcaaac
     3781 cctgcaggtt attgtaatga agaaactctc gcatttcatc ggtggctatg taccatgttt
     3841 tcggtatctt gatttcatcg agtaaagata tccgttcact ttcacgcttc agtacctcct
     3901 ttgccaagaa taaacctgtt gcctttccac caatctttcc atggcttcct gcagggtaaa
     3961 tcagcgtctg gacaatgtcg tagaagccac gaatgtcgag gtattgttgg gcaatccgta
     4021 tgaatccagg tttgtccgac aaaaaacgac ggattaaggc taccctaagc cagcgttccg
     4081 taggcggatc cagcatattg accgtacccg ctgtatttgt atacctgctt accgcttcaa
     4141 ccacatcacc gatggaagta tcgatacggt cgatagtctt tataagcgta tatgcttttg
     4201 tctcctgtat ccacttcatt aaccttgtgg agatttcacc atccgtcata tgcttggatg
     4261 caatagagaa ggctttttca cttatctttg ccagatctcc aaggggaagc atttgacttg
     4321 gaaaatttac ttctcctgca acaggaaaca cctttgaacc gaaattaagc ttgcacaact
     4381 cctgctctgc ctctctaatc ccgcgatagc agagatgatt aaccatcttc ctgcaaatat
     4441 gaagaagcat atcctggtcg gttttctgta agagtgctat tattgccatc cagtcggcag
     4501 cggtaggatg ttttggaaga ccatcctttt ctggaatgtc ccactcttct attaaacgct
     4561 ccgctcttct gaaaaaaatc gtttgttcaa tacggtctgc tattgttttg atgagtcttt
     4621 tttctttttc gagaaagtag ccctcatccg tcttcgaaac ttcatcaatg tatgttacgg
     4681 tgatccgccc aacaacttta ccacctgcct tgatgtctac tccatccgag atccgtgatc
     4741 ttgcaaaatc tgcagactga tacctgtggt tgtcgtaggc gatctccacc cggcaaactt
     4801 cagggaacct gaatccagag ggcagtacaa ggacaatccg ttcaaacatc tcagtgagag
     4861 ttagcccact gttacctagt atctcatcga cctgatagag gcaatccagt tctttggctc
     4921 tctctttcag ggtatcgagt gaggctcctt tagggtcgct actatcctcc attaatattc
     4981 ccctttctca ggattcacct tgttatgccc accctcgcat tttgactgcc tgcgccaccc
     5041 ggttgattgc aatgacatag gctgcatcac gcatataaag gtttttcttt tttgcaagat
     5101 cgtacacagc ccgatacgcc gaggtcattt ttgtatccag tttttccagg acttcttctt
     5161 tctcccaata ataattcaag tttgactgga cctgttcaaa ataactacag gtaacaccac
     5221 cggcattcgc aagaaaatcg gggagtagga aaatgcctcg ttcttttatg atgtcgtctg
     5281 catccgtggt ggtaggtccg ttggcacctt ccaacaggac tttaacctgt ttacttattt
     5341 tgttcacatt tttcgccgtg atctgattct ccaaagcagc aggcataagg atatccacat
     5401 cctgttcaat ccattcctct ccaggtagga ttttacaacc agcagcagct gcttttttct
     5461 tgttgatgca cccaaaagag tcggttattt cgaggagcat gttcattgaa aggccttctt
     5521 tcttctgata agtatatgac actttatctt cattgtccca gcaggcaact gcaacaacct
     5581 tccctccaag ctcggtaaac attttagcgg catactgagc aacatttccg aatccctgta
     5641 cgctgagact tgtttcttcc aacttgattt gcaggtcctt tagggcttct cgcaagacat
     5701 atatggctcc gaatcctgtg gcctcggtcc ttcctgttga accccccata ccaaccggtt
     5761 ttcccgtaat gaacccagga taccttcctc catgaatggt ttcaaattcg tctaacatcc
     5821 atagcatgtg tttcgcagta gtcatgacat ccggtgctgg tgcatcaatg tatggcccca
     5881 tctctttgga tagctgccta acccaccccc tgcacagccg ctcctgttcc ctctcgctca
     5941 ggttatgggg atcgcaaatt acccctccct tgcctcctcc taacgggata tcgactacag
     6001 cacacttcca ggtcatccac attgccagcg ctcttactgt atctgccgtt tcctgaggat
     6061 gaaagcggat gcctcctttt gctggacctc ttgcatcatt gtggagtaca cgaaagcctt
     6121 taaatatctt cattttgccg tcatccatgc ataccggaat caagacatga tgttccttta
     6181 agggctggcg cagcaaatcg cacgttgcct cgtcgagacc gataaaagag gctaccttat
     6241 cgaactgcgc ctgggcattt gtaaaggggt tataagaact cttttccata tatacttcct
     6301 ccttctttca tatattttca caaagacata gaagcaacct attctacaaa aggttgcatc
     6361 attatttatc tttaggcaat gtccatagaa cactccatat cttcccatgt ttcattagct
     6421 tatctttgat agtctatcgg tattcttcac aacaccatac ttctcccctg ttctttggga
     6481 atggacagga atcgtaaatg gcacaatcac tgcaaaggcc tctgtttttc aggtactcag
     6541 gcctcacagg catgtattga gacccttcgt cagacttagc ctctaaggaa ttcttctcac
     6601 acataccact ctcctgtgtc ggatcgtatg ccctgaattc ctcacactcc aacgcccatg
     6661 ccgcactcga attgacaagg acacagtaag gagcagcgaa acagaatgta cacaggtcca
     6721 tcctgtcttt ccctctcacg ggaagtgtat cgcctttctt ttttcgtgca gaaaagttct
     6781 tcattttcag cctcccctgt tttcgaagtt gaagacaaaa tccgttaagc aatagacatg
     6841 ccagcctttc tgagaaacat ataacagaac agtgatagag agaacattat aaaatccata
     6901 catttatatg tctgttcgca gagtatgtcc aatggttaca cacgtataat gcgatgacat
     6961 aaaccgggga aattctaccc aattgggact atttctccat ttgagtgttt tcatcctgac
     7021 tatctaaacc tttacagact gactactttt ttgcagtttc ttacgaagag tcttacggtc
     7081 tattccgagc acagcagccg ctctagtcct attcccctta acagaagcaa ggacgttgcg
     7141 aatgtatcgt gcctccacat cagcaagagt ccgggtaaaa tcttcctcct tacagaccga
     7201 aaatcgaaga agctgcggta aatcaggtgt ttcgacaata tctccatcag taaggacgat
     7261 gaggcgctgt aaaacattct gcaattctct cacgttccct ggccaatgat aattttgaag
     7321 cacctgcaag gtttgcgatg aaaaagccgg gaccggtttg cccatctctt cagctaatcg
     7381 ctccgaaaaa tggcgtatca acatgagtat atcatcaccc cggctcctta agggcggcag
     7441 ttcgatagcc aacacattga ccctaaaaaa aaggtcttgt cggaaaagtc ctttcttaac
     7501 aagggcataa aggtctttgt tcgtggatgc tacgattctc acatcgacct ttcttgtgcg
     7561 gcttgaaccg accatgcaga cctctttatc ttgaagcacc ctgagcagct taatctgcat
     7621 attcaagctc gtttcactga tttcatcaag aaaaatcgtg cctccgtccg ctgtctgaaa
     7681 gaaacctgca cgtgaatcta tagctccggt aaaagcacct ttcatgtgtc caaagagttc
     7741 gctttcaagc agttcttcgg ggatagcacc gcagttaaca gggacaaagg gtgctgatgc
     7801 tctgttgctg ctgtaatgaa cagccctagc aaccaactct tttcctgtac cgctttcccc
     7861 ggcgatcagg actgttgccg acgtcaaagc tgcttttgca atgaaatctc taacacctgc
     7921 catagccggc gactcgccaa tgagaccgta ggaggtggtt attttttttg tgggaaatct
     7981 gcttgctgcc cttcgggtat ggagtttacg gagcgtactg gccacggctg aaaatagttc
     8041 ttcatcggta aaaggttttg ccagatactc ttccgcacca gtttttatcg cctctactgc
     8101 acccttcacg ctcggatatc ctgtaattat tatcacttcc atatcactga agttctcacg
     8161 gatatgtctt aagagatgca ccccgctcaa accgggcatc ttaagatctg taatgacgag
     8221 gtcaataggg gtaacatcaa ggattttgat agcttcagta acattcaacg cagcaaaaac
     8281 catgtacccg gcgctcacca ggttgcgatg gagcacctcc agtgttgcag cggaatcatc
     8341 tactatgaga atacggtctt tatcgtccgt acaacgtacc atacgtttat ctatcagggt
     8401 gatcttcttt tcttaaaggc agctcaattc tgcactttgt gcctctccct accttacttg
     8461 caacttttat ggaaccccca tgggcagtaa caatgccatg aacgacaggt aaccccaatc
     8521 ctgttccagt tccaatatct tttgtggtaa agaagggtgt gaatattttt tgtagaacat
     8581 tctttcccat gccagctccg gtatcctcca ctaaaaggca ggctttcgct tggtccttta
     8641 gtgtggaaat tgttatttta ccgccctttg acatggcctg tatggcattg actatcagat
     8701 tcacaaacac ctgatgtaac tgtgaagggt ctgcatcaat ctttggcaag tcaggctgga
     8761 gtgcacattg gaactctatc ccatcactgg cacactttga ttcaaataag ccaatcgcat
     8821 tttttacaat atagttgagg tctactttgg ttttctgtgg tgacacctga cgggaaaaaa
     8881 caagtagttt tctcactatt tcgcgagcat gaaaagatga attgactatc ttatcaacat
     8941 ctgcctgcac ctgatcagga agtccgggat tcttttttat taactgagcg aaaccaagta
     9001 tgtgattgag cggttcattc agttcgtggg caacaccggc tgcaagctga cctattgttg
     9061 ccagccggtc tgcgtgtcta agttgttctt ccagattcag tttctcctgt tctgcttctc
     9121 tccgcataat gattactgca atctcgtttg caacagcatc gagaagggcc cgctcttcgt
     9181 tgagaaatgg tccttcatca cgttctggct tttcttccac atatgagatt tcaacataac
     9241 cacgtctttt gttgcgaatg acaattggcg tcctctgtcg atgcctaaca cgtttagggt
     9301 attcctttgc tgagcgagaa tgaccatcga gaacgattct ggcacaagct atgtccgggt
     9361 ataaccaggc agagggaaga atcccggtaa taccctcaag tactttgcca agagctgtgc
     9421 cagcattggc ggcaaggtgg gctatgcggt acagacagga gagttcttta acgcgctcca
     9481 gtaaatttaa ttttgtttgt tcattgagat ttgcacatgc tttcatttta tttcattgtt
     9541 caagtttaga caatacatgg aattatttca agaaaagata ttttagagta acgttcacaa
     9601 ctgtttgcca agaccccaag gggtgttgta tgctttgtta tctatggacc ttttcggaaa
     9661 tgagactcat cttgacgcga gtatacaaaa acctcttgca gacagaatga ggccgaaaaa
     9721 cctcgacgaa gtagtcgggc aggaacatct acttggaaaa gggaagatac ttcgggttct
     9781 tctcgacaaa agggatgtgc cctccttaat cttctgggga cctagcggtg tagggaaaac
     9841 cactctgggc tggctcattg gaaaacacat gaagctacct tatgtttcgc tgagcgcggt
     9901 caacatcggc attaaagaag taaaggaaat tattcagaag gcgaagcacc agaagataat
     9961 tatctttata gacgagttcc accggttcaa caagctccag caggacacct ttctacccca
    10021 cgtagagaat ggagatattg tccttattgg tgctacaaca gaaaatcctt catttgagat
    10081 catttcccca ctcctttccc gaatgaaagt gcttaccctt aaagctctcg aaccgaaaga
    10141 ccttacaaca atactcaggc gcgcactcaa cgaagaccat gagctcaaaa atgcaacgtt
    10201 gaggatagat gaggctacta ttgaggaact tgccctcctt gcaaatggtg atgccaggaa
    10261 agctctcaat ctcttggaag tatgttttaa aatggctaaa aaagagggta aagaagaatc
    10321 tgttattgat cgcaatgttc tacgagaagc ataccagaaa aataccgctg tttatgataa
    10381 aaaaggtgag atgcactacg atcttataag tgctttccac aaaagtttgc gggggtcaga
    10441 cgctgatgcc tctctttact ggctggcacg catgattgag ggtggtgaag accccctttt
    10501 tattatgagg aggatggttc gattcgcatc cgaagatgtg gggaatgctg atccccaggc
    10561 attgatgctc accatggctg ccatggaagc cttcaatttt atcggcccac ctgaaggcta
    10621 cctggctatt gcccaggcct gtgtctatct tgccctctgt gaaaagagca atgctatcta
    10681 tgtagcatat ggtcgggcaa gttcagacgc acgggagctt ccggagtacc ctgtacctct
    10741 tcacattcgg aatgcaccca caaagctcat gaaggaactg ggatacggtg aaaattacct
    10801 ttatccccat aatttcgaga atggccttgt atcacaggaa tacttccctc ctgagataaa
    10861 agagaaacat tattactttc cgagcaacag agggtatgag cagaaacttc tcgcatttat
    10921 ggacaaggtg agaaaagtaa aaggaaaaga cagcttgtag ctcatggctg atggtaaaaa
    10981 acatagacaa ctcaacaagg gataagaaaa ggaaggaatg gagataccag aaaacaggct
    11041 catagacaaa atcagaagat tcgcggatag atcgagggat gtcttgcggg gaattgggga
    11101 tgatggggct gtgataaagc tttctcaagg ttcttacgtg cttgttcagg atgctatggt
    11161 tgaacatgtc cactttaaat tctcctttac aaacccatat gacgtgggga aaaaagcagt
    11221 ctatgttaac atctcggatg tgctgtctat gggtgctctg cctctctatt tcctcgtcac
    11281 cattggaatc acgcctgccc tcacatcgaa agacataggc cgtctctatc atggcatgag
    11341 acaggcagca agggagttcg gtgcaatcat gataggtggc gacaccacag agtccaaaag
    11401 tgatttcttt atagatgtat ccatgattgg aaaacttgtt acaaaaacct accttgggag
    11461 ggacagggct aaagaaggag atctcatcgc agtcacaggc tccctcggcg agagtgctta
    11521 tggccttcat cttttgttgg agggcaaacc aacaaaaggc atgtcaacct ttcttaaacg
    11581 atatgcgagc cccaagcccc cataccccgt atggaaagaa atagtgaaac atgatataac
    11641 aagggcaatg atggacataa gcgatggtct tcttatagac ctcggaagaa tgatggccga
    11701 gagtaaaaaa ggcgctgtta tacatcttga aaaattaccc ataccacccc ttctccgaaa
    11761 aaaaaacaag gaagttctcg ctctctcagg aggggaggat tatcagcttc tctttacctt
    11821 tccaaaaagt aggctcaaaa agattcaagg aatgatacaa cagggttatc ctatcagcat
    11881 tattggtgag gttaaacaag ggaaagatgt aacagtcctt tctcagggaa aggtctatga
    11941 agcccctgaa aaaggctatg aacatttcgg aaactcccgg tgaaaaaaca gatcttcgtc
    12001 aaagacatta caaaagagta tatggatatc aatgattttt ttgttgttac caaaaaaggg
    12061 acttttttga gaaagaataa cataaaatac atgactatag gactgagaga tacaacaggg
    12121 gtcatagagg gcaagatctg ggaacgggtc gatgagtttc aggatatgtt tgacaagaat
    12181 gatcttgtct atgtgaagtc aaaaagccgt ttttaccagg ataaacccca attgaatatt
    12241 acagatatca aaaggattga agaacccctt accatggatg aactgaaagc gtttttccct
    12301 gaggggcagc gggcaagcaa gacagtacgt gaagaattcg ctgagctcgt gaacagcatt
    12361 cagtccccct acattaagtc actcttctct gcattcacag aacaaagtgg tctcgcagat
    12421 aaattctact tataccctgc ttctataggg gtccatcatg tttatcttgg aggactcctt
    12481 gaacactcag tctctgttgc ccagatggca aaagaggctg tgaggttctg tggcggtaat
    12541 ctggacattg tgattgcggg cagtcttctt cacgacatcg gcaaggttga tgagatggaa
    12601 attaagggaa cattccgtta ttcagacaac ggacggcttt taggacatat taccctgggt
    12661 gttatcatct ttgactcatt agtaaaaaaa ataagggatt tcccgattca gattagcgat
    12721 atcatcaagc atattattat cagccaccat ggcgtagaag agtgggggtc accaagaaaa
    12781 cccatgacca tcgaagcctt gatcgtacac tatattgaca atcttgatgc caaggttgcg
    12841 ggcgtgaaag agtatatgat ggaaaacatg gaggatggga catggaccac ctttcaccgg
    12901 ctctatgaat cgcgttttta taaaacccag gaaggtgtct atggagatta gaagggtctt
    12961 tgtcgataag cttaagataa aaaacaacct tgtccttatt accgggccaa tgcagaacta
    13021 tatcagcagt gttttgagga aaaatactgg tgaccgtata gatatcattg atggaaaagg
    13081 ctacctttat cgttgcactg ttaacagcat caaaagtaag gaagtattcc tccagatact
    13141 tgatgttgtt catcgtcctg aagaaaagcg acccaaagtc acgctctgtg taagccctat
    13201 taaaggccca cgaatggatt ggcttattga aaaagctacc gagcttggcg cagatcgaat
    13261 cctgccaaca attttcaaaa gaacattgat taggatcgaa gataaggaaa aggagaagct
    13321 cgagagatgg aggagactcg ctattgaagc gtccagacag tcaggacggt ttaccattcc
    13381 ggaaattatc ccgcccactc cactccgggg aataactacc cacttgaatg gcgtacaaaa
    13441 taaatgggtc ctctacgaaa aagaaaagaa aacatccata aaagaggtta tatcctcaca
    13501 aagagaaaat ggcatctgta ttgtaatcgg tcctgaaggc gggttagagg agtccgaaat
    13561 ctcatggcta acagagaacg gttttatctc ttgcaccctg ggcgaaagca tattcagagc
    13621 tgaaacaacg cccttggtgg tactctcaat aatcctttac gaatacagta aaaaatgaga
    13681 aaagcatcaa tccttttcag attctttgca tttgtcgtag acattcttgt gctctttttt
    13741 ttcatatctt tgttgtttgc cgccattgtt acaggatacc tgctctcttc cggatctatt
    13801 cttattcctc aaatttccct actgactttt atctttgcat gtggttccct attcacattc
    13861 attttttact tcacatacct aacaatgaac ggaggcgcaa ctgtaggaaa acgttttttt
    13921 caattaagag ttctgcgtca agacggttct ggactgaacg ccggaagggc cttcatgcga
    13981 accatgatct acccgttatc gcttactcta tggtttgtca acgtgctatc cgctcttctt
    14041 ttggacggcc gtatgataca tgatattgtc gttggatcat gggtagtcag ggtggaatta
    14101 tgaaccacag gggcatggca ggatgtggtt gtctcatagt cattcttatc attatcatgc
    14161 tgtttacagg tgcgctgatg caccccatta cgcttcgttt tctcggcacc caattccatt
    14221 acgaagacag gatatttacc tcagatgcca tctttgtgcc aagatttcca gaggacagaa
    14281 atggcgagct ctatgcagcg tcttttcagg atttctctgc gggtaatgga aaagcaatac
    14341 ttgttgagga tgatactgtt cttgggacaa gcattgtcga actggtcaac aagatggcaa
    14401 aagcaagaaa cgtgaaagat gacgggataa agagaatatc cgcgtcgggt gaaggattgg
    14461 taaaagccca aagggttaag gaacagatcg aaaaacaagg attaagaaaa ataatcatta
    14521 ttgtgcctga ctatgcttct cgtcgttttc acctcatgta tgactctgcc agagctggcg
    14581 gaaaaacact ttatctcata aaacctgttc cagttacata tttcaacaga gacaagtggt
    14641 ggaaagattc aggctcaaga ggtatccttg cagaggaaat cttctcgata agtcattact
    14701 accttcaaag attcaagtat ggagaaaagg aaatgaaaga tagagagaaa cgatgatcac
    14761 tgcggcaggc atctgctttg cctgttttcg tgttctccct tcaattttgg cgttttcaac
    14821 tttgaaaaaa cttttataaa actgataaga tccgatcaaa ctgaagggaa ccacacaata
    14881 tacaaaaaag tagaagacat gaccggacag catttccacc tcagcgactg ggataaccag
    14941 gagattgtga gacaggaggc gtagaagagg cctaacatca gaccatggac gataaagtag
    15001 ccatcattgc cctacacagg gtggagggaa taggctccgc cataaaacgc agacttgttg
    15061 atatgcacga gaaccttgca cctctttttt tagggaaagt aaagcttggt gatgacaggg
    15121 tatacaaaaa gattgtggcc ttcaagggat ggaaagacat agatcgggat atgaaaaaaa
    15181 tcagcgacat gggggccgaa atattaacca taaaggatag agaatatcca gaccagctcc
    15241 gtaatattcc agatgcaccc atcgtgatat acaaaaaagg aaggctgaag attggagcag
    15301 acactattgc tattgttggc tcccgtgcag catcatttga gagtataaat atggcagaaa
    15361 agattgctca gactctctct tcgtccggaa tcacagttat aagcggcctc gcacgaggag
    15421 tagatgctgc cgcacacaaa ggcgcactca aagagaaagg taagactgtt ggcgttctcg
    15481 ggtgtggcat tgacatatgc tatcctgcag aaaacaggcc tctcttcaaa aaaattgaag
    15541 aagatggttg catcctaagt gaatatggac tgggagaacc tccattaccg caccactttc
    15601 ccatgagaaa cagaattatc gcaggcctgt caaaaggtgt actcgtcgtg gaagcttctc
    15661 agcgcagcgg ctccctcata actgctcgcc ttggcctcga atacggcaga gaagtcatgg
    15721 ctataccggg caatattttt gatgaaggat acaggggcac aaactcgctt atcaaacagg
    15781 gcgccaaact gatcgatggg atagaagaca ttattaccgc atgtttttct ggcttaacct
    15841 tcaaaacgga taagcacgtt gatttagaca acaatgaacg ttatatttat gggattattg
    15901 ggtccacaaa gattcatgtt gatgaagtgg tagaaaaaag cggtatggaa acgaagcatg
    15961 taatggctat tctcacaaac cttgagctaa aggaagccat aaaaggtata ccaggtggat
    16021 tctatttaag aatataatcc agctcgcagc tcatgactga tagctgacag caaaattggg
    16081 agtaaataca tgggtaaatc attattagtg gtcgagtcac caacaaaaat gaagactcta
    16141 tccaaatttc ttggcaaaga ttatgtgata aaagcaacct atggtcacat caaagatctg
    16201 ccaaagagta aactgggcgt agatatcgaa aaaggattca ccccacagtt cctgactgtg
    16261 aagggtaaga caaaggtagt ggatgaactc aaaaaagtgg gaaaggatgc tgagaaaatt
    16321 cttatagggt ctgaccctga tcgtgaagga gaagcaattg cctttcatgt tgccgatgtt
    16381 gtgggtaaga aaaaagaggt gaaaagggtt cttttccatg aaatcacgaa aaagggtgtg
    16441 ctcgatgcat tgaaaaatcc gaccaccctg gatgagtcaa aatacgatgc acaaaaggcg
    16501 agaagaattc tagacaggct tgtagggtac aagatcagtc cactcctatg ggagagggtc
    16561 agttacggac tttctgctgg cagggtacag tctgtagccc tgcgcctcgt ctgtgacagg
    16621 gaagaagaga ttgaggcctt cgtaaaagag gagtattggg ttatagacgt tgaactacaa
    16681 ctcaactccg gagagatctt tactgcgaca ctcgaaaaga aagaaggcga aaagatcaaa
    16741 attacctcaa aagatgaggc agaagccatc aaaaaggcga ttgaaggaaa aaccctgctc
    16801 atagacaaga tcgaaatcag ggacaagcat gtttatccac aacctccatt catcacgagc
    16861 agactgcagc aggaggcttc cagaaaacta aaattctctc ccaagagaac tatgatatta
    16921 gctcaaaagc tctatgaagg ggttgacatt ggcgaggaag gcgtaacagg gcttatcacc
    16981 tatatgagaa ctgattcggt gagagtttca aatgaagcag tcaaggatgc cagaaagttt
    17041 attgtgaaca gttttggaga accctatctt ccaaagatac cacatttttt tcgaaataga
    17101 aaaactgcac aggatgccca tgaggcaatc agaccgacct atgttgaact gagtcctgac
    17161 aagatcaagt cgcatatgga caaagacatg ctggcccttt atgagcttat ctggaaacgg
    17221 tttattgcgt cccagatggc ccaggaaaaa ataaaaacaa aaacagtcga aatcgaaggt
    17281 ggggattaca cctttgttgc tcacggatca gaagtcatct tcgatggttt tacaaagatt
    17341 tatgaagaag aaaaagaaga tgaagaagaa gggacttacc tacctgatat gaaacagggg
    17401 gaagaggttt ttctaaaaga cacaatcctt aaccagcgct ttaccatgcc tcccccaagg
    17461 ttctctgaag cctctctcat aaaaacactc gaaaccaagg gaattgggag accctcaacc
    17521 ttcgctacca tagtaagtac tgtccaggaa agggcctatg taaagaaaga taaagggagg
    17581 ctttttccta caccgcttgg cagaaccgta aacaagcttc tcaagcagtt cttccctctc
    17641 atccttgata ttgacttcac agcaaaaatg gaagacagac tcgacctcat tgaggtcggc
    17701 aagaaaaact ggatacagtc acttgaaaag ttctatgagc tttttgataa agacctctca
    17761 gcagctcagg aggggatgaa aagcctgaag aaggaagaga cagagacaga tattatctgt
    17821 gataaatgcg gtaaaaccat gcttcttaga tggggaaaaa atggtgaata tttggtatgt
    17881 agcggcaaac ccacctgtaa aaacaaaaaa aatgtgaaag ttgaagataa tgggaccatc
    17941 aggatcgtgg aagctgaaat tatgggaaca tgtggacaat gcggaggcaa cctcattgaa
    18001 aagaggggca ggttcggaag gttcatcgca tgcagtaact accctgaatg taagtataca
    18061 gagcctttca atatcggttt ccagtgccca gaagaaggtt gtactggaaa actcattgaa
    18121 aagatctcaa agaaaaaaaa gaagttcata agctgctcca gatatccaaa ctgcacgttc
    18181 gccaccaaca cggagccaac tgaaggccca tgtccgcaat gtggggcacc cacccttttt
    18241 tcttctagaa aaaaaacccg ttgccttcgt aaggactgtg gatggacatt acagtagtag
    18301 gaggtggtct cgccggagta gaggccgcct accagctcgc cagccggggc cattctgtca
    18361 gcctttttga gatgaggccc accagatact cgcctgccca taaaacacca cttctctccg
    18421 agcttgtctg cagcaattcg ctcaagtcca aggagcttac caatgcacac ggcctgctta
    18481 aagaggagct cagaatactg ggatcgatag tgataaatac agcagatcag gcagcaatcc
    18541 cagggggaaa agcccttgtt gtagacaggg agatatttgc aaaaagaatt accagggaaa
    18601 ttgaggccaa tccacttatc actgttatca ggagagaaat ccaggatatc ccggccggga
    18661 ctgtcattat tgcttctggt ccacttacaa gtaatgccct tgcaaaaaga ctggcagaga
    18721 ttacaggtgt caataatctt ttcttttttg atgctatctc gccgatcatt gatggcggga
    18781 cagtcgatat ggataaggca ttttttggat ccaggtatga tgagaatagc gatgattacc
    18841 tgaattgtcc catgacagga gaggagtatg atgcatttta taatgccctc ataggagctg
    18901 aaaaagttga tttgcagccc tttgagaaaa catcattttt cgaagggtgt ctcccagttg
    18961 agatcatggc acaaagaggt cgtcagaccc ttgcttacgg gcctatgaaa ccggtagggc
    19021 tcaaggataa aactgctggc aaagaaccat atgctgtcct acagctccgc agagagaaca
    19081 tggctggcac aatgtacaat atggttggct tccaaacaaa gcttacctac gcagaacaag
    19141 acagggtttt caggatgatc cccgccttac gcaatgctgt atttctcaga tatgggagta
    19201 tacaccgaaa cacgtatatc aactcgcctg cccttctcaa taactctctt tgccttaaag
    19261 gccaagacca gacattcttc gctgggcaga taacaggcgt tgaaggttat gtagagtctg
    19321 ttgccatggg tcttgtagcc ggaatttcag ctcacgtaag catcatgaac caagtttttt
    19381 caccacctcc ggaagatact tgcgttggtg ccctgctcaa ctatatcact acaccaaatg
    19441 ataactttca gcccatgaat atcaactttg ggctgataaa aaattaccga aagagagcga
    19501 aagaaaaggt tattcataat gctctccaag caatttcgtt atggaatgaa gaaatcgatg
    19561 agatcagtaa aaagtgaaaa gcaagaagtg ctaacactgg ctctttttgg aggattgaaa
    19621 tggaatctaa gataaaaagg ttggcgacaa aggccatcaa caagaaaggt atttctgagg
    19681 acgatgcaaa atcgctctat gagataggga aggattatcc tttcatggta ttcgcctatg
    19741 gatcagaaat acgggaacat ttcaagggca aaaagatcga tttgtgttgt atcatcaacg
    19801 caaaatcagg catatgtccg gagaattgca ggttctgtgc tcaatcagcc cattatcata
    19861 ctactgctcc cgtgtatcca ctcttgtcag cggctgagat cataaaaaaa gcacaggaag
    19921 caaaggagtc gggtgcacat tttttcagta ttgttacgag tggaacagca ataggaaaca
    19981 aggaagagtg gaatgaaatc tatgaagcta taaagggtat cgcggctctt ggcttaaagc
    20041 cctgtggctc cctaggtatg ctgaccgacg aacaagcatc agcattgaag aattcaggac
    20101 tttaccggta tcaccacaac ctggagactt cacgaaccta ctttaaaaaa atatgctcca
    20161 cccatgacta tcaggatgat atagaaacaa acatggcggc aaaacgggcc ggcctatctg
    20221 tttgctgtgg ggggatcatt ggtcttggag agacaatgga ccatcgcatt gaaatggcaa
    20281 taaccctgaa agaaataggt gtagattcag tgccgattaa tattctcaat ccgatcccag
    20341 gtacaccgtt agaggggaat ccccctctct ccccctttga aatacttttg acagttgcga
    20401 tttatcgttt tattttgcct gataaagata tcaggctttg tggaggaaaa gaaaaaaacc
    20461 tacgtcaact attgcctctt gcaatcatag cggggtgtaa ctctcttatg acgggcaatt
    20521 atcttaccac gcttggaagg gaaacagcac aagacgtgga gatgattcat gacctcggac
    20581 tgagagccac atacggctga tacctatgtt acacctgtcc atctgaaata tatctctggt
    20641 ttatcttgct ttttgtgctt gatttttacc gaaattccca aactcaacgc tgcaaattat
    20701 tgcgcgttat cctactgcct gtccgagttt aaagatgggc aggtacatag caatgatgag
    20761 cccaccaagt acaacaccaa gaaacatcat gatgagtggt tccatgagcg atgtcaggtt
    20821 cgtgacgaga ttgtccacat cttcttcgta aaaatctgca atcttgttca acatattgtc
    20881 taacgcacct gtggattcgc ccacctggac catttgtaca accataggag ggaagatacc
    20941 gctatcctga agtggttccg acatgctttt gccctcgctg atttttgccc gggctactat
    21001 gagggcatcc tcaacaatct tgttttttgc caccctggct acaatcacaa ggctttcaag
    21061 gattgcaacg ccactggaaa gaagggtggc cagggttctt gttactcttg ctaccgacga
    21121 cttgagcgcc aacacaccaa aaacaggtgc tttaagtacc agtctgtcta tggttctctg
    21181 accactttcg gttttgtagt atctccggaa aaggaaaacc ccaatggcca gggcaataat
    21241 catgtaaatt atgtaagatt tcacaatttt gctaatatta ataacaacct gggtaggcaa
    21301 aggaagctcc gctcccatat ctttaaacat gccttcgaac actggtataa caaaaatcaa
    21361 caacaccatg accacagcga tggcaacaac gataatgctg atcggataaa tcatcgctga
    21421 ctttattttc tttttcagct tctcgatttt ttccatatag atggccagtc tgttgaggac
    21481 tgtatcgagc ataccaccct cttcacctgc caccacaagg ttcacgtaaa gctcatcaaa
    21541 gcatttcggg tagtctctca gcgcatctgc aaacctggaa ccaccctcta tttttgcctt
    21601 tatttctttt acaataccac agagattctt atcttctatc tgattagata ggatttcaag
    21661 cgactgaaca aggggtaggc ccgatgtgat catcgttgac aattgtctgg tgaaaagaac
    21721 tactgcaagg ggcttgatct tcttatttgt attgaccttg acagccttct ccccgccctt
    21781 tttctcagta attttggtaa ctattacgcc ttctttgcga agcgttgtcc tcagtatggc
    21841 ctcggagtcg gccttcaacg tcccgcttcg tgcctcacct ctgccagttt taccttgcca
    21901 ttcgtatagt ggcataatgc tatcttacct tatcaaaaaa aagtttgaaa gaaaaaagca
    21961 ccagatgcag tctcgtttta acatcgcaga ataaccttct atcatagtcc atacacgtga
    22021 caaatgacaa ttatcaccga tctttttatc ctcggagaac cgtaaagaat gtggtattgt
    22081 ctctttgtaa gaggattacc gcaccttctt taatattaac tgttttcatg gcctccttga
    22141 aatcttctat attctttatc ggctttctgt taatttcttt aataatatct cctgatttta
    22201 tatcaccctc ctgagcaggg cttcctggtt gaatatcagt tacaattacg ccacgtctgt
    22261 ctttgatgtt gagatgtttg gctatttctg gcgttatgtt ctggacgact aggccgaaat
    22321 ctttctcaac ttcgggcttt tttgatgcct gaatcttttc atctttaagt tcgccaatta
    22381 caacgtccac ggcaagagat tttccttccc tgatgattcc aacttttgcc tttttcccaa
    22441 cttcagtccc accaacaagt tttggaagag tatctacgtc cttgatatct ttcccattaa
    22501 aagacacgat cacatcacct cttttgatcc cggccttatc tgcaggacca ccttcggtaa
    22561 catcggaaac aagggcgcct tctgattctt tcactccaaa agttctggct atttcaggtg
    22621 ttatcttctg tatgactact cccagccaac cgcgcgtcac tttgcctttg ctttttaatt
    22681 gtgggagaag ttcttttgcc atatttatgg gaatggcaaa cccgatcccc tggccacccg
    22741 agacaatggc tgtattgatt cctacaacct ctccattaag attgaagaga ggtccgccac
    22801 tgtttccagg attgatggaa gcgtctgttt gaataaaatc atcgtaaggc cctgcaccaa
    22861 taaccctgcc ttttgcgcta acaattcctg cagttacagt tgtttcaagt ccaaatgggt
    22921 tgccaatggc tacaacccaa tcgcctatct ccagattatc tgaattacca aaacttgcag
    22981 caggtagact gttccttgca tctatcttta tcaatgccag atcggttttt ggatccctcc
    23041 ccactattgt tgcatcatat tcctttccat cgatcaattt tactctgatg gtttgtgctt
    23101 tctccacaac atggttattc gttataatgt atccttcccg gtcaatgata cagccagatc
    23161 caagactcct ttgcttgaac tcccgttttt gagaatcacc aaaaaatctt tcaaaaaact
    23221 cctcaccaaa gaaatctttg aatggatttt gctggccgaa gaacctgtcg tgcatattag
    23281 gccctttgat cacgctcgtt gtgctgatgt tcactaccga gggctttaca gattttacca
    23341 gcccactaaa gcttggcaag cccttatcaa aaaccacatt ttttacattt gactgattag
    23401 cgtcaaacca cgtatcgcct gtgttcacct tacctctcag gtacccgaac gcaagcacca
    23461 cgatgatgag agcaaaaata gtgaaatacc atcttttgtt tttcataaac tcctccggac
    23521 tatataggca attttattgt aaacatacta ccctgggctg gctgactctc tacctcaatt
    23581 ccaccctgat gagcctcggc aatccaacgg ctgatggaaa gcccgaggcc actgccgctc
    23641 gccctttttc gtgatcgatc ggccctgtaa aacctatcga agatatactt aatgtccatt
    23701 tctgatattc ctatcccatt gtcttttaca tagatatttg catatccgtt attcgcatga
    23761 gatgatacgc ggactctccc tcccgcgtgc gaatacttaa ttgcattttc aacaatgttg
    23821 aggagcatcc ttcgtagctt taattcgtct ccttttaacc ttatatcttc gagttcgtta
    23881 gcctctagtt caacgttttt tttatcggca actactttca tatccataca cacatctaag
    23941 atcaaatccc tcagggaaac ttcctccaca tcgagtttta tctttcttgt atcagccttg
    24001 gagagtagga gtaaatcatc aatgatgcag gacattctgt ctatctcttc gagattactc
    24061 ttgagaagta cctgatagtc ttgcttttcc ctctccttgc ggagtgctac ttccgtttct
    24121 ccctttaaaa tggtgagagg ggtttttaac tcatgtgaaa catcgatgct gaactggttt
    24181 attctctgga aggtgtcttt cagcctgtcg atcatagcat tgaatgtttc tgccagccga
    24241 ccgagttcat ctcttcttcc ggaaacatct attctctcat caaggttact cgatgatatc
    24301 tttactgctg ccctccttat ctgatcaacc ggtcttagcg cttttttcgc cataaagtaa
    24361 ccaacaacta tggttacgca gattgatgaa ggtatactga taatcataat cagcaaaagc
    24421 ttttttattg tctcatcaaa ctcctccagg gatgttccaa cctgaactac actcgttact
    24481 ttcttattct caattatagg gattgttatc atccgcaggc ggggttttgt cctatctgtg
    24541 gtttcataga caacctcgcc tcgcattgct ttttcaaggg tactgaagct tgtcggcagg
    24601 gcttctcctt cgatatcgct catttttgca ccgattctcc ctgaagagtc cataatctgg
    24661 ataaatttcc ccttgggttt cttaccaagc acattctcca aatatcgctc gaaattaccg
    24721 aatacgcttg tattgtgtga gtctgtcatg gatgatgaca gaacttcgcc tatggaacgt
    24781 atttttgtat caatgctttc ttgcaggctg tttttgaaat agatgaatat tccactggca
    24841 aaacctacga gaatgagaga gagcacagca ccataccaga tagtgagacg ccattttatc
    24901 gggaggttaa cctttttcat catcttcctt taatacgtat ccggttcccc tcactgtgtg
    24961 gatgtatctc ttgcttgtca cagactcgat cttttttcgt aaaaaattta cataaacgtc
    25021 aactatattg gttgaatctg tctggctttg ccagacataa tctgcaattt ccttccttgt
    25081 caatggcttt tcactatttt tgagcaaaaa ctccatgata aggaattctt tctcagtcag
    25141 ttcaactgct ttcgtttcta tgtggagttt cctggtatat gggttcagga taatgctctt
    25201 atagtctatg atatgggaat ctgaaatttt gctccttctc aggagagcac ggattcgagc
    25261 gagcaattca tcaaaggaaa agggtttggt aaggtagtcg tcgcttcctg tatcaagccc
    25321 ttttaccaca tcttcttttg agctttttgc agtaataata agaacaggtg tattattgcc
    25381 ccatcccctg atagtcttga ggagatccag cccgtctatt tccgggatca taaggtcaag
    25441 caacaaaagg tcgtaggtag actccttcag gagttcaagc cctcttctgc cgtcataagc
    25501 aatatctatc gtataacctt cttcctccag gcccttgctt atgaaatggg cgaccttttc
    25561 ctcatcttct acaagaagta acttcatggc tcttgttagt ataacaaaaa tcgtttattt
    25621 gctgcaactc atgggcaata caatatagat cgggtaacca tataaaaagt ttgacaaact
    25681 attcgcaata ctattaagta tatagtctgc catgtcgttg agcagaggtc agcagttatc
    25741 agcgatgagt atccggaaaa actgtttagg tccgtgtctg ttatgctgtg agctgattac
    25801 cgacaccata ttttatcatg gaggttattc tcgatgaatt tgctggaggc ttacatcgtt
    25861 atgtttattg caagtttctg caccctcgtt attgagatgg ttgctggccg aattctggcc
    25921 cctttcgtgg gtgtttcaat ttacacctgg accagcatca taggtgtaat cctcgccggc
    25981 atcagtattg gggcatatat tggaggcaag ctcatcgaca agtttcccgt gagaaaaaca
    26041 ttgggttggc tgttgctttt atcgggcatt tccgcgctta ccattatccc acttaccaac
    26101 cttattgcag cgtatcgctt caatatgcca ctcatgatga gaatatttct cgtgactgcg
    26161 gtaatcttct tccttcccgg ttgtatcctt ggaacggtct cgcctgttgt ggtgcgtctc
    26221 acgctgaaaa accttgagaa cgccgggaac gtaatcggta agatttatgc tgtatcaacc
    26281 cttggagcaa ttattggaac atttgccacg ggttttctct tcatctcgtg gatgggaaca
    26341 cggagcatta ttctgaccat ggctataatt ctcatacttc ttgctctttt ttgcggtgcc
    26401 cttttcaaaa ctaagaaagc tcttgctgca tttattgtaa ttgccgccct gtgtatctgg
    26461 ggggtctatg cctatgcata caaaatccca agcagagaaa acatgtatct ttataaagag
    26521 agcgattatt acacaatcaa gctgctgaca acaacaagtt cggatcaaaa aacgccatta
    26581 caggcgatgg tgctcgacaa ccttatacat tcctatgtct gtcttgaaaa cccatttcac
    26641 attgagtatg aatatgagag gatatattcg gatgttctta agtggaagtt cagaaaaaat
    26701 gaggatttta agagcctcac gattggaggc ggtggctata cctttccaag gtatatggag
    26761 ctcttttatc caaatgctca gattgatgtc gttgaaattg accctgaagt caccaagatc
    26821 gtttacaacc acctcggttt atctgctaat acaagaatca aaacatacaa cacggatggc
    26881 cgctggtttg tcatgaattg cccagaaaaa tatgatctta tttttacaga tgcatacaac
    26941 gatctctcaa tcccctatca ccttactaca aaagaattcg ccatgcagct caaatcaatc
    27001 atgaagcccg atgcccttct catgtcaaac atcatagaca atttccaggt tggagccttc
    27061 cttccttctt acattagaac tttgcgcgaa gtctttggcc acaacaacgt ttacctcttg
    27121 tctgtaagcc ctgattttga aaatactcgt attagcactt tcattgtaat agcaggaaac
    27181 ggtaagcttg acataaagga cttcgatgcc cacgtcaaaa gctatcagca gaagcatgcc
    27241 acgtctgtca ttgtacccga taatttgatg gaggagttca tgacaaaacg ctcgtccata
    27301 ataatccgtg acgattatgc gcctgttgac aaccttattg caccaatctt cgaggaaaga
    27361 ttcggatata ataggaagag ctgataaaag caagcgatag gtaagaggtt ataagcaata
    27421 gataaaaatg gatattgctt tacaggaata aatcctataa tattatttgt gggcgggcat
    27481 aactcagttg gtagagtgca agcttcccaa gcttgatgcc gcgggttcga gccccgttgc
    27541 ccgctccata tttctccaat gatttcaacg cctgtaccct taaaaactcg tttctaagtc
    27601 gtttcggtct gacttaattc tgacccccta tttttctata cattaagcag tcactcatat
    27661 attaaggtac aaaaagatac cttaactact ttaaggcgat actttgctgt gttttattat
    27721 tttggcttct cgggataact atctttcttt ggagaactat ccacggagta tgttttctta
    27781 aattcagaaa agacaagcac ggcagtaaga acagcaatga caatggttag tatcagaatc
    27841 cataaagtca ttttgctcat ccttttttcc ccttccttct gccgtttatg aagaataaca
    27901 tctgcttctg ccataaggtc agcaggagtg gtggtgctct ttgcactcat aaaatggggc
    27961 attttgccat tcttaattct caacaactct tcgtcagact tcttattcaa atcttctaat
    28021 gaatatgtca tcgcagatgg acaccattta aatggctgct tttgcctttt actcttgagg
    28081 gatttgctac gcctattttc atcttttaag ttgatttact tggctgttca aacacgcgtt
    28141 gtacgcagca ggtccttgga ggtaggcact gctacaggct gcgctggctg aattcctctc
    28201 ttcagaagac aatccggaga aatcaggttt ctctgttgac cc
//
LOCUS       DAOOMC010000022        26495 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdus sp. isolate AMR_MDS_2685
            NODE_1671_length_26495_cov_6.277950, whole genome shotgun sequence.
ACCESSION   DAOOMC010000022 DAOOMC010000000
VERSION     DAOOMC010000022.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864760
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdus sp. (wastewater metagenome)
  ORGANISM  Syntrophorhabdus sp.
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae; Syntrophorhabdus.
REFERENCE   1  (bases 1 to 26495)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 26495)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,729
            Genes (coding)                    :: 2,708
            CDSs (with protein)               :: 2,708
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 7 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26495
                     /organism="Syntrophorhabdus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1671_length_26495_cov_6.277950"
                     /isolate="AMR_MDS_2685"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2699756"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(3..1187)
                     /gene="hisS"
                     /locus_tag="PK125_03680"
     CDS             complement(3..1187)
                     /gene="hisS"
                     /locus_tag="PK125_03680"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: protein motif:HMM:TIGR00442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HPB37241.1"
                     /translation="MEKIKTLRGFRDISGEELEKFRRIEAVVRKYLQLLNFTEIELPI
                     LEKTELFVRSIGDTTDIVEKEMFTFRDIGGDSLTMRPEATAGMVRAYLQAGMYATERV
                     SKLFTIGPMFRHEKPQKGRFREFRQIDVEVFGIDDPLIDAELLWMIHVILKELRVNNY
                     EIEVNSVGCPGCRESFRKVLVEYFVGKKEMLCDDCVRRLDRNPLRIFDCKNSECIEVS
                     VGSPLLFEYLCGDCRHHFDEFQGHVDSFGFPVKINKRLVRGLDYYTRTVFEVTSNDLG
                     AQKAFMAGGRYDNLVEAMGGPKTPGTGFAIGVERLALITMREQVLTVPTYFFAYLGER
                     AKTYLIPFIKSFVEAGLSLNYSYDGRSLKSQMRYADSGMPTLFLYLVMTKWTRVLSFC
                     AT"
     gene            complement(1378..2358)
                     /locus_tag="PK125_03685"
     CDS             complement(1378..2358)
                     /locus_tag="PK125_03685"
                     /inference="COORDINATES: protein motif:HMM:NF013087.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008171 - O-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HPB37242.1"
                     /translation="MISEMVMAEVMAFMKSRVILTAAELDLFTNLDDRPQTAADLAQK
                     QGLDIRATTRILDSLSSMELLAKTDNIYRVTDKGIFLSAHHPETILPMVLHMTHLWHA
                     WSGLTEVATNGPGKQPDSGLKFSEKEWKSFVSAMHVAARALSKEIADSYDASGFNRLL
                     DVGAASGTYTISFLHKNPQLMAIVFDLEDVIPMAKERIKEEQLFHRVEFIAGDFYEED
                     LPKGCDLALLSAIIHQNSPEENLKLYGKVFRALEKGGTILIRDHIMSEDRTKPPAGAL
                     FAVNMLVNTRGGDTYTFQDVKDGLENSGFREVQLLRTGQKMDCLVEAKKP"
     gene            complement(2490..4748)
                     /locus_tag="PK125_03690"
     CDS             complement(2490..4748)
                     /locus_tag="PK125_03690"
                     /inference="COORDINATES: protein motif:HMM:NF009414.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional dihydroorotate dehydrogenase B NAD
                     binding subunit/NADPH-dependent glutamate synthase"
                     /protein_id="HPB37243.1"
                     /translation="MAREQKLNEIVEKRDLAPSLTLFKLYVPDIAKKVKAGQFVVLRA
                     DDYAERIPLTVAEYDRDAGLITVIFQVVGSSTQKMAKFEKGQTILDVVGPLGKPSHIE
                     KFGTVVCVGGGVGVAPVLPIAKALFEAGNKVISIIGARTKEMLILEEEMRKVSTELYV
                     TTDDGSYGHHGFVTDVLKQIIEKGEKIDEVVGIGPVIMMKAVSDVTRPHNIRTIVSLN
                     TIMVDGTGMCGCCRATIGKETKFVCVDGPEFDGHQVDFKELIARQKMYNREERRALWD
                     HQCKLEFQAKQLKKTKRRERMPEQDPKKRIQNFNEVALGYTRENAIREASRCLQCKNM
                     PCVEGCPVNITIPAFIKKIKEGDFMGAIHVIKETNALPAVCGRVCPQETQCESKCVLG
                     KKGEPIAIGRLERFAADYEAQQGDIRVPEIAKPTGKKVAVIGAGPAGLTVAGELSKKG
                     HEVTVYEALHKAGGVLVYGIPEFRLPKAIVQREVDYVSKLGAKIKVDVIVGQAVTMDE
                     LFEQGFDAIFVGTGAGLPYFLNIPGENLNGVYSANEFLTRANLMKAYLFPEYDTPVRV
                     GSKVAVIGGGNVAMDSARVAKRLGAEDVYLVYRRSRAEMPARAEEAHHAEEEGIDFRL
                     LTNPIRVVGDEQGWVKGIEVVKQQLGEPDASGRRRPIDIPGSNYMIDVDVVIVAIGQG
                     PNPILTQSGAGLELKKSGNIVADPETGKTSRKGVFAGGDIVTGAATVILAMGAGRKAA
                     AAMDEYLRTGQW"
     gene            complement(4801..6288)
                     /locus_tag="PK125_03695"
     CDS             complement(4801..6288)
                     /locus_tag="PK125_03695"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HPB37244.1"
                     /translation="MAETASILVVDDERGIREGCRRILMSEGFSVDVAENGEEGLKST
                     KARPFDLILVDLMMPVMSGLEMMEQVRQHDPEIIMIVITGFATIETAVEAMKHGAYDY
                     VPKPFTPDQLLAVVNRGLEKRRLTLHAQKLMEERDQKLLEVANEESKIHAIVNSMADG
                     IIVTNKEHQLVLWNPAAVKMLNLDKKLEAGKDIGETIQVGTLINLINKALSPDSSQYT
                     AISEEIELANPDKRILMANVSVIRDKDGQDLGVVSTFRDITALKELDQLKSQFVSMVT
                     HELRAPLSAVEGYLSAYLSGAAGNDPHMNRQMLERAKARTHSLLDLVNDLLQFSRLES
                     KKVERKKELLDLTDILINTVELLRNQGEANEITFSLNLPEKLPLVEADRSEMEQLFTN
                     LISNAIKYNVKKGKVAISAGPTNHYLSIKIEDTGIGIDKEHLTSVFDEFYRICGPETR
                     YITGTGLGLSIVKRIVESHFGFIEVDSNAGKGTAFTVKLPIKKEL"
     gene            complement(6309..6713)
                     /locus_tag="PK125_03700"
     CDS             complement(6309..6713)
                     /locus_tag="PK125_03700"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HPB37245.1"
                     /translation="MSKKTKILLVDNDVDFIDLNKAVLENSGFDVVVAYSSAEGLDRV
                     RFEQPDLIVLDLMMEKHDSGFGFAKAMKADPTTKNIPILMLTAVGSETGMEFSQELDG
                     YWMKTDDYANKPLLPEDLVKKINELLAKSAKE"
     gene            complement(6732..8807)
                     /locus_tag="PK125_03705"
     CDS             complement(6732..8807)
                     /locus_tag="PK125_03705"
                     /inference="COORDINATES: protein motif:HMM:NF014904.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-Fe] hydrogenase large subunit C-terminal
                     domain-containing protein"
                     /protein_id="HPB37246.1"
                     /translation="MTITQPDKRLNPYDIDVNNRKCRRCSYCVQICPANAIKVGRDGF
                     QVIQERCVLCGGCIRACPQGALTYHGGLSKVKQFFVEGKKTIAVLDPSFPVETDFGKP
                     RQFVSALKQIGFDEVWEGAFGAELVSQVYFKMLHEEAVRPLISSFCPVIFFYIQKYYP
                     QLIPNLAPVVSPMIAIGRVARELKSPDWKVVYLTSCLARMGEIVAPEVAGAIDEVITF
                     VDVRNLLIEAGVDKASLPETEFDGPRPYQGRIIPVIGGLYRNIGPSFDVLIDEISVIG
                     GPRRTTSSLKQLAEGYMEGKFLDFVYCYGCVDGPFAQRDHSILARRQIVAQYTKSEMA
                     KQDVVSVMADLDRFKNIDLGRSFQNMEQRLPSPTEEEIRAVLKRIGKLPPNRNLDCRA
                     CGYPTCRDKAIAVVQGLSPEEHCLPHLLGESKKIYQQLEKSHRELQISHQELEQAQAQ
                     LIRTEKLASIGQLAAGVAHEINNPLGTITIYAHLLQKSLESDDPRREDVDLIISEAAR
                     AKDIVQGLLSFAREKKLKATEIKVSDILEEVLSLVINQPLFRNIKIEKSFDPGLQTIV
                     ADEAQLKQVFLNIIMNAAQAMEGGGKLLINVSSGKKHIKIRIQDTGPGIPPEVMRHVF
                     DPFFTTKEKGTGLGLAISYGIIERHQGKVDVETELGKGTTFVITLPVKASEDQVLDEI
                     KAKIHGFKI"
     gene            9059..9556
                     /locus_tag="PK125_03710"
     CDS             9059..9556
                     /locus_tag="PK125_03710"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HPB37247.1"
                     /translation="MAIKDVMKEVMLIVEAIEESDLFKGVSPEFLKELAQIGEMRIFK
                     RDSIICRANEKARYFYQLLEGQIDVMLSEKEIIHFTLNRPGEIFGWSSLVEPYMYTAT
                     AKCNTDVRVVRMARESLEKTMIKYPADGLAILKHLTSIISQRLRHAYLYIYSKELNKN
                     KVSEK"
     gene            complement(9886..10029)
                     /locus_tag="PK125_03715"
     CDS             complement(9886..10029)
                     /locus_tag="PK125_03715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB37248.1"
                     /translation="MFSGSGRGMENHEVYIERCVDMERRHLVLTKNIFWITDGRKGII
                     SDL"
     gene            10028..10258
                     /locus_tag="PK125_03720"
     CDS             10028..10258
                     /locus_tag="PK125_03720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB37249.1"
                     /translation="MLHTILIKTKCSHIRLSSPMGCADEESEDDDKVFSHDQRKVFCS
                     DGMWQEMDEGSLVFCGAKVQKPNASKRSCGSE"
     gene            complement(10393..11805)
                     /gene="gltA"
                     /locus_tag="PK125_03725"
     CDS             complement(10393..11805)
                     /gene="gltA"
                     /locus_tag="PK125_03725"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016291044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004355 - glutamate synthase (NADPH)
                     activity [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent glutamate synthase"
                     /protein_id="HPB37250.1"
                     /translation="MSEEKKSRIDTNRRDMPKQTPEMRRHNFNEVAFGYTEELAVAEA
                     SRCLQCKKPRCKTGCPVEIDIPDFISCIVKRDFRGGIRKLKEKNCLPAVCGRVCPQES
                     QCESQCILKSKGAEVAIGRLERFLADWEAAQDEVDLPPKARPTGKRVAVVGSGPAGVT
                     VAGDLVLLGHEVTIFEALHKAGGVLTYGIPEFRLPKTIVAREVEYVKQLGVRVITDYV
                     VGKTRSIDELLDRYDAIFIGTGAGLPWFMNIPGENLNGVYSANEYLTRMNLMQGYQYP
                     ASRTPIKRHKKVAVVGGGNVAMDSARTALRMGAEEVRIIYRRSHAELPARAEEAENAE
                     EEGVIFNMLTLPVRYIGDENGWLKEIECLKMELGEPDASGRRSPVEIPNSNFKMEMDA
                     VVCAIGNSPNPLVPSTTPGLETTRRGTLVADPETGKTTRERVWAGGDIVTGAATVILA
                     MGAGRKAARSIHEYLTTGKQ"
     gene            complement(11802..12659)
                     /locus_tag="PK125_03730"
     CDS             complement(11802..12659)
                     /locus_tag="PK125_03730"
                     /inference="COORDINATES: protein motif:HMM:NF004862.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfide/dihydroorotate dehydrogenase-like
                     FAD/NAD-binding protein"
                     /protein_id="HPB37251.1"
                     /translation="MNKVIERTMIVPNMHLLTVEAPEIAHKIRPGQFVIVRVNEEGER
                     IPLSVADWDLDTGSITIIFMEVGASTGALAELKAGDVIPTCVGPLGNATEIKKYGTVM
                     CVAGCYGIGSIFPVARELWHAGNQIIMVFEARSSYLLYWADRYVSLGAKIFNITRDGS
                     LGIKGHVPHLTDIIHSLPSLPDQIVVNGCTFLMAHTSKVTKNLGIPTVVNLNPIMIDG
                     TGMCGVCRVTVSNKMKFACVDGPEFDGHEVDWVEFLARRKQYINEETSYFRHSEPKPI
                     EHKEGKCHS"
     gene            complement(12656..14923)
                     /locus_tag="PK125_03735"
     CDS             complement(12656..14923)
                     /locus_tag="PK125_03735"
                     /inference="COORDINATES: protein motif:HMM:NF024900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HPB37252.1"
                     /translation="MTKEKKISITIDNKQVEVRPNITILQAARDNNVYIPSLCALEHL
                     PSYGACRLCIVEVDGLRGFPTSCTTPVEAGMVVRTDTAEIRGLRQEILKLLLSEHPAS
                     CLFCDEHDECKKFMGTIRKVGVITGCRYCPNDARCEIQEIVDKVGLTETSYPVYYRGF
                     PVEKNDPFYDRDYNLCILCGRCIRVCNSIRFNGTLAFKQRGKLTTIGPAFDRTHLEAG
                     CEFCGACVSVCPTGALSTKTSKWYGIADAQVGTTCTFCSVGCRLLLQVKNDEVIDVLP
                     DYDDTVDQGLICVKGRFAIPEYVNSPQRNTTPQKLTPVGYEVITWDQAFDLAAEKLSG
                     VKPDDILVVISPQLSNEDLFVAQRFVRQVLGSPPMMSSVLFDLGNDISSFFDLVLYTS
                     TYDSLETAQGILSVGFDSTYGYTPIGIAAKRAAQRGAVLVTLGSCDSNLDMLSEASFK
                     MEPSRWAGFLDVFTDSLARKGKGKNRGTKEFAGNLNEVTEALSGSSANVLIMGPEVIF
                     SYRRNEVFERIKVLRDRLGWKIIVAHPYTNLIGMLAMGAFPGVKPGELLQNGSKSIKL
                     GLPSLDPTKRKKLVYLVGEGRFDIIPDCDYLIYQNAIMPSSWRQPDLILPTSLFPESP
                     GTTINVERRILTLEKATGQYMDSRADWWIMTRIADRMKKGKFKYKDINSIQNEIKKQI
                     PNFKASKKKVDFVKIDFKNGGKWLKGSTKVEPESLPSATYSSYRGIPLAQVVGGMKII
                     EDWDRSSSFSDKEAQ"
     gene            complement(14920..16800)
                     /locus_tag="PK125_03740"
     CDS             complement(14920..16800)
                     /locus_tag="PK125_03740"
                     /inference="COORDINATES: protein motif:HMM:NF013662.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-ubiquinone oxidoreductase-F iron-sulfur
                     binding region domain-containing protein"
                     /protein_id="HPB37253.1"
                     /translation="MNKINKKISLAKKKWKDLEDNTEPIIYVGAASCGRAAGALELLD
                     GIDQFLVENKVFARVIQVGCIGPCYLEPLVDIKLPEQPRVSYNNVNMKMLPAILKSHL
                     LQGVPHTKLALGNFGNESFYDIPRFFDLPMLKPQVRIVLRNCGFVDPEDIDQYLALDG
                     YRGLINALKMSPEDVIGVVRQAGLRGRGGAGFPTFKKWTVCRNSPGEQKYLICNADEG
                     DPGAFMNRSLIESDPHALLEGMLIAGYAIGASKGIIYIRAEYPLAIERLKKAIGQMRE
                     YGLLGNKILDSDFSFDVKIKEGAGAFVCGEETALIGSIEGKRGMPRSRPPFPAISGLY
                     GCPTIINNVETLGTLPNILRNGAEWYTKFGKEGNRGTKTFSLVGKVRRPGLIEVQLGT
                     PLREIIFDIGGGVQKNFKAIQTGGPSGGCLSEEFLDLPVDYESLASVGSIMGSGGLII
                     MDDDTCIVDVARYFLDFTQKESCGKCVPCRVGTRHMVELLDKITHGDGTPEDLMALRT
                     LGDTIKRGALCGLGQTAPNPVLTTLRYFRNEYLEHIKDGRCRATVCKDLIEYRVIKEK
                     CTGCQSCVRVCPTGAISGPRSEPHNLDPTKCIKCRSCYEVCRFEAIAGDAIVIRSAEK
                     RT"
     gene            complement(16793..17242)
                     /gene="nuoE"
                     /locus_tag="PK125_03745"
     CDS             complement(16793..17242)
                     /gene="nuoE"
                     /locus_tag="PK125_03745"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF005722.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HPB37254.1"
                     /translation="MPEPIESILAKHDRKQEELIPVLQDIQKEYGYISPESVKKISRY
                     LRISENQIYGVSSFYAQFRFTEPGRHSLKVCLGTACHVRGGATLLEMLERSLGIHCGE
                     TTSDKRFDLERVACLGCCALSPVVQIDKEIYSRMTVNKLTEHLKDYE"
     gene            complement(17488..19020)
                     /locus_tag="PK125_03750"
     CDS             complement(17488..19020)
                     /locus_tag="PK125_03750"
                     /inference="COORDINATES: protein motif:HMM:TIGR00368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HPB37255.1"
                     /translation="MISKIITATIYGIDGIKIDVEVDISLGLPAFNIVGLPETSVKES
                     KERVRSAIKNAGFEFPNDRITINLAPADVRKEGSSFDLPIALGILTALNIIKKDTISN
                     FLIAGELSLDGRIKSVRGILPTAILARKEKIANIIVPHENGREASVVQDIKIFGAKHL
                     LEIVHFFKGGESLEEFRTVNAQGEDGGTGTGLNFSDIKGQAQTKRAIEIAVSGSHNLL
                     MIGPPGSGKTMLARRIPTIMPPLSYDEAIETTKIHSIAGLLSASKFLVLNRPFRAPHH
                     TISDAGLIGGGHVPKPGEVSLAHNGVLFLDEFPEFKRNVLEALRQPLEDGDVTVSRVT
                     HAITFPARFMLIAAMNPCPCGYWGDSRRSCTCTGSQIHRYRSRISGPLLDRIDLHVEV
                     PPVTIRELSLDKEEESSYEIRDRVVQARQTQEQRFAEKKIYANGQMSARMIKKYCHLT
                     ENGRNILEKAVEKFGLSPRAYHRIIRVARTIADMEKEDHVSESHIAEAIQYRVLDKRL
                     TL"
     gene            complement(19125..20246)
                     /locus_tag="PK125_03755"
     CDS             complement(19125..20246)
                     /locus_tag="PK125_03755"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanylyltransferase"
                     /protein_id="HPB37256.1"
                     /translation="MKRNSSVTPHQAADISVLDHTYCIIMAGGRGERFWPLNTHNMPK
                     PFLKILGAKTMIQLTVERVAKLVPLDHIYVVLGKSHVDIARDQLPDLPLNNFLVEPEG
                     RDTAPCIGFAAISLLEIDPQAIMIVLPADHFIPDTESFHKTVLYGTDIASKGDYLVTI
                     GIKPDRPETGYGYIKAGEIIFSSNHQACYAVTKFVEKPDAEHAQLYLEKGNYFWNAGM
                     FIWKVKTVLAGIAEHMPDLYKGLLAIQTYKADRNEELFVEIYSNLVRKSIDYGLMEKA
                     QNVVMVPGEFTWDDVGTWSSLLRLKELDLNGNYCSGDTVCIDARNSLVCSEGLKVGVI
                     GLSNVVIVASCDGVLVCNAERAQEVREIARLAGERKDSK"
     gene            complement(20284..21021)
                     /locus_tag="PK125_03760"
     CDS             complement(20284..21021)
                     /locus_tag="PK125_03760"
                     /EC_number="2.3.1.286"
                     /inference="COORDINATES: protein motif:HMM:NF001753.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0017136 - NAD-dependent histone
                     deacetylase activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent deacylase"
                     /protein_id="HPB37257.1"
                     /translation="MDDYKAIAKIIKEKGRMVAFTGAGISVDSGIPAFRGGQGLWEKY
                     DPMEYAEIRAFNHNPEKVWVMLREMSEVIFDSRPSPAHLALGTLEKKGILKTVITQNV
                     DGLHQVAGNTDVIEYHGNHRWLICPNCSKKIPLAREVVSVYPYPRCEKCNMALKPDVV
                     FFGEGIPMIAAFRANEEANVCNTLFIIGTSGVVYPAAGIPFTAKSNGATIIEINVEET
                     PFTSSITDHFLKGSASEILPKILEYLE"
     gene            complement(21141..22118)
                     /locus_tag="PK125_03765"
     CDS             complement(21141..22118)
                     /locus_tag="PK125_03765"
                     /inference="COORDINATES: protein motif:HMM:NF015535.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P1 family peptidase"
                     /protein_id="HPB37258.1"
                     /translation="MLNSITDVKGIKVGHASDFKGYTGCTVILCEDGATCGIDIRGSA
                     SGTRQVDALGINHIVEHVHAILLSGGSSFGLDAATGVSRYLEEKGVGFDVGPTKIPIV
                     PTAVIFDILFGDPKARPTLELGYEACMNAGDTVEEGSVGVGTGAAVGKIFELSRAMKG
                     GVGTSSVVMPDGLVVASLVVVNAFGDIIDNVTGKIIAGARVAPDSLEFANTVESIKKG
                     AVKKQFGLVNTTLGVVATNAKFNKKEITKVAQIAQGGLIKTINPVHTTFDGDLVFALA
                     TGVYEADINLVGVLGEFVIAEAIKRAVKKADGFGIVPAFRDITKGWKTT"
     gene            complement(22150..22650)
                     /gene="purE"
                     /locus_tag="PK125_03770"
     CDS             complement(22150..22650)
                     /gene="purE"
                     /locus_tag="PK125_03770"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: protein motif:HMM:TIGR01162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HPB37259.1"
                     /translation="MEKPKILVLIGSDSDLPVIKDGLEFLKEVGIPFRLDISSAHRQP
                     EKTINYAQRARTEGIEVIIAVAGMAAHLPGVVASHTTIPVIGVPTSGGVLNGIDALLS
                     IVQMPKGIPVATVGIDASRNAAILACKILSIKYDEVRKKLDKMKSDMKKYNEDKSKKL
                     KDILED"
     gene            complement(22834..23019)
                     /locus_tag="PK125_03775"
     CDS             complement(22834..23019)
                     /locus_tag="PK125_03775"
                     /EC_number="5.3.2.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016862 - intramolecular oxidoreductase
                     activity, interconverting keto- and enol-groups [Evidence
                     IEA];
                     GO_process: GO:0006725 - cellular aromatic compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxymuconate tautomerase"
                     /protein_id="HPB37260.1"
                     /translation="MPIIEVHMFEGRTVDQKRKLVAEMTDAVVKSIGVKPEDVRIILQ
                     DMARYDYSIAGKLAIDR"
     gene            complement(23365..24153)
                     /locus_tag="PK125_03780"
     CDS             complement(23365..24153)
                     /locus_tag="PK125_03780"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HPB37261.1"
                     /translation="MIDFDKGLERWDHPEIVSRLFFPRREFSGEPNHLKAKNYFVEMD
                     DGITIGCRFYPAQSNGPNILYFHGNGETVFDYDYIAPLYQKRAINLFVADYRGYGMSD
                     GSPSSGSMIRDAHCIFHSFIDFLHDMNFSGNLFVMGRSLGSAPAIEVAYHYQQQLNGL
                     IIESGFASGRNQLMRLGMSHLFRDVQDPIGFGNHLKIKEVTIPTLIIHGDEDDIIPVT
                     EGRMLYDLSGSQEKYSLYIPHAGHNDIMIIGLDMYMKSIAKFTE"
     gene            complement(24558..24911)
                     /locus_tag="PK125_03785"
     CDS             complement(24558..24911)
                     /locus_tag="PK125_03785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB37262.1"
                     /translation="MRFLVLLVAIFFVFAIPIHSSAQPWRRGMYDKYERAPYGQFCPG
                     MRGDPYGARRQIRTVNEARHALEQYFKGIGKEVGIGKLEERRWFFIAELLDSDGFLID
                     KAIIDKRTGRIRSIY"
     gene            complement(24975..25406)
                     /locus_tag="PK125_03790"
     CDS             complement(24975..25406)
                     /locus_tag="PK125_03790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB37263.1"
                     /translation="MKKVLAVALAVVFGTLMSSASFAAWTKGQSPGPGVQVDVNAFRN
                     FQKETLSLRDEMAVKRLELRNEFIKETPDQARIATLQKEMIDLSTKIREAASKNGLPD
                     RGFGFGFSKRGSYGPGYGRGHHMGWGGGHGPGYGRGYCPNW"
     gene            complement(25476..>26495)
                     /locus_tag="PK125_03795"
     CDS             complement(25476..>26495)
                     /locus_tag="PK125_03795"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPB37264.1"
                     /translation="RELKEPGEFFPYPLFLSGEQIGTLEARELRPLREGIFLSRSNRF
                     LLMAIFIIGGIAIILSVLFSKRLTSPIKELARAASAISKGDFRKRVSITRHDEVGELS
                     YTFSHMAQALEKQEILRKKLIADVAHELRTPLGVMRGELEGMIDGLIPNDIDHLQSLH
                     DETGRLKKIVDGIEDLNQAEASILSLQKQRFKVKPFLENITERYRNIFLEKGINVEVQ
                     CPEDFEVEADPERLSQIIMNLINNASRAMDSGGLVSLNVSSGPMGLKITVDDTGTGIA
                     EKDLPFIFDRFYHGSGGGLGIGLTIVKELVEAHGGKVEAKSILGKGSRFSIVLPTGGV
                     HNSSQ"
BASE COUNT         6840 a         6622 c         5308 g         7725 t
ORIGIN      
        1 tttcatgttg cgcagaatga taataccctt gtccacttcg tcatcaccaa gtataaaaac
       61 aaagtcggca ttccgctgtc cgcatacctc atctgagatt ttaacgatct tccgtcatat
      121 gagtagttaa gggaaaggcc tgcttcaaca aatgatttta taaaaggaat aagatacgtt
      181 tttgcccttt ctccaaggta tgcaaaaaaa tatgttggga ctgtcaatac ttgttcacgc
      241 attgtgataa gcgccagcct ctcaacacca attgcaaagc ctgtgcccgg tgttttaggg
      301 ccgcccatcg cctcaacaag gttatcgtat ctccctccag ccataaaagc cttctgagca
      361 ccaagatcat tcgatgtaac ctcaaagact gtcctggtat aatagtcaag tcctctcaca
      421 agcctcttat ttatttttac gggaaaacca aaactgtcta catggccctg gaactcatca
      481 aaatggtgcc tgcagtcacc acacagatat tcaaacaaca aaggtgatcc gacgctaact
      541 tcaatacact cgctgttttt acagtcaaaa atcctcaagg ggtttctatc caacctcctc
      601 acacagtcat cacacaacat ctcttttttt ccgacaaaat attccacaag aactttacga
      661 aatgattcgc gacagccggg acatccaaca ctgttcacct ctatctcata gttatttaca
      721 cgaagttctt tcaggatcac atgtatcatc caaagcagtt ctgcgtcaat gagtggatca
      781 tcaataccga aaacctcaac atcaatctgg cgaaactccc tgaatctccc tttttgtggc
      841 ttttcatgac gaaacatggg gccaatggtg aagagtttac tcactcgctc tgtagcgtac
      901 atgcccgctt gaagatatgc ccttaccatt cctgcggttg cctccggacg catagtaagg
      961 gaatctccac caatatccct gaaggtaaac atttctttct caacaatgtc agtcgtgtct
     1021 ccaatacttc gtacaaacaa ttctgttttt tcaagtatgg gaagctcaat ctcagtgaaa
     1081 ttaagaagct ggagatattt acgcaccact gcctctatgc gcctgaactt ttcaagctct
     1141 tctcctgaaa tatccctgaa tcctctgagg gtctttatct tttccatgta tttatctccg
     1201 aattgctata aaatcaatac attactatca caaacatgca tcatttaaaa gtggtttttc
     1261 aaatctttaa ttcaggcgat gacgtggcct atcagttatt cattctgctc ttttgaattt
     1321 cctgtacctt aattacctgt tgcctatgaa ccatcgccta ttggctgtct tcgaatatta
     1381 cggcttcttc gcttccacca gacagtccat cttctgaccg gttcttaaaa gctgtacctc
     1441 gcgaaatcct gagttttcga gaccatcctt gacatcctga aacgtgtacg tatcaccacc
     1501 gcgggtattg accaacatgt ttacggcaaa gagggcccct gcaggtggtt ttgtgcggtc
     1561 ttcactcatg atgtgatctc tgataagtat tgtaccgccc ttttcgaggg cacggaaaac
     1621 cttgccatag agcttaagat tttcttctgg actgttctga tggatgattg cagagagaag
     1681 ggcaagatcg catccttttg gaaggtcttc ctcataaaaa tctcctgcaa tgaactcaac
     1741 gcggtggaat aactgttctt ctttgattct ttccttagcc atgggtataa catcctcgag
     1801 gtcaaacacg atagccatca attggggatt cttgtgaaga aaagatatcg tatatgtgcc
     1861 tgatgctgca ccaacatcca ggagccggtt aaatccgctt gcatcatagg agtcagctat
     1921 ctcctttgac agggctctcg ctgcaacatg cattgcactt acaaaagatt tccattcttt
     1981 ctcgctgaat ttgaggccgg aatcaggctg ttttcccggt ccgtttgtgg caacctctgt
     2041 gaggccactc cacgcatgcc aaagatgtgt catgtggagg accatgggca ggattgtttc
     2101 aggatgatgc gctgacagaa agatcccttt gtccgtgact ctgtatatat tgtcggtctt
     2161 tgcaaggagc tccatagagg acaggctatc aagaatccgt gttgttgctc ggatatcgag
     2221 accctgtttc tgggcgagat cggcggccgt ttgcggtctg tcatcgagat ttgtgaaaag
     2281 atcaagttcc gccgctgtga gtatgaccct gctcttcata aaagccatta cctcagccat
     2341 aaccatctca gaaatcatag attataccct tctcttattt ttccagctcg caatggctat
     2401 gatacattaa ataggtatct catatggcta tggaaaaaag gccgtccaga ctctttccgg
     2461 acggccctct gtttatccat gcctctttgt taccattgcc ctgttctcag gtactcatcc
     2521 atagctgcag ctgctttacg gccagctccc atggcaagga tgactgttgc cgcacctgta
     2581 acaatatcgc caccagcaaa tacacctttc ctgctcgttt tcccggtttc cggatcagcc
     2641 acgatgttgc cggatttctt caattccagc cctgccccac tctgcgtcag aattgggttc
     2701 gggccttggc caatggcaac aattacaaca tccacatcaa tcatgtaatt tgagcctggt
     2761 atatctatag gtctccttct tcctgatgca tccggctcgc ccagttgctg cttcaccacc
     2821 tcaatgccct ttacccatcc ttgttcatca cccacaaccc ttatcgggtt tgtgagaagt
     2881 ctgaagtcaa tcccttcctc ttcagcatga tgggcttctt ctgctcttgc cggcatttca
     2941 gcccttgaac gacgatagac aagatacacg tcttctgcac caagtcgttt agcaaccctt
     3001 gctgagtcca tggcaacatt ccccccaccg ataacagcaa ccttacttcc aacccgaacc
     3061 ggagtatcat attccgggaa caggtatgcc ttcatcaggt ttgctctcgt aagaaattca
     3121 tttgcagagt agacaccgtt cagattctcg ccggggatgt taagaaagta gggcaatcca
     3181 gctccggttc caacgaatat tgcatcgaat ccctgctcga aaagttcatc catggtcacc
     3241 gcctgaccca caattacgtc taccttgatc ttagcgccga gtttactcac gtaatcaact
     3301 tctctctgga cgattgcttt ggggagacga aattcaggga ttccataaac aagaaccccg
     3361 cccgccttgt gaagtgcctc atagactgtt acttcatggc ctttcttcga aagctctccg
     3421 gccactgtta atccggcagg acctgcacca atgaccgcta ctttctttcc tgtgggcttc
     3481 gctatttcag gaaccctgat atcaccctgc tgcgcctcat agtcagctgc aaaacgttca
     3541 agccgaccaa tggcaatagg ttcgcctttc tttccaagaa cgcacttgga ctcacactgt
     3601 gtttcctggg ggcaaacccg accacataca gctggaagag cgttcgtctc tttaataacg
     3661 tggattgcgc ccataaagtc cccttccttt atctttttga taaacgcagg gattgtgata
     3721 ttaacgggac agccttcaac acaaggcata tttttgcact ggaggcaacg ggatgcctcc
     3781 ctgatcgcat tctccctcgt gtagcccaat gcaacttcat taaagttttg aatccttttc
     3841 ttgggatcct gttccggcat cctctcacgt ctcttggtct tcttcagctg tttcgcctga
     3901 aattcaagtt tacactggtg gtcccagagt gcccgtctct cttcgcgatt atacattttc
     3961 tggcgcgcta taagctcttt gaaatcgacc tgatgaccat caaattcagg cccatcaaca
     4021 cagacaaatt ttgtctcttt accgattgtc gcgcggcagc aaccgcacat gccggtacca
     4081 tctaccatta tagtattcag actgacaatg gtcctgatat tgtgaggcct cgttacatca
     4141 ctcacagcct tcatcataat tacgggccca atccccacaa cttcatctat tttttcaccc
     4201 ttttcaataa tctgtttaag gacatcagta acaaaaccat gatggccgta tgaaccatca
     4261 tcagtcgtca catagagctc tgtgctcact tttctcatct cctcttcaag gatgagcatt
     4321 tctttggttc tggccccaat tatggaaata accttgttcc ctgcttcaaa aagggccttt
     4381 gctatgggaa gtaccggtgc aactccaacg ccacctccta cacagaccac cgttccaaac
     4441 ttctctatgt ggctcggttt tccgagaggt cccacaacat caaggatggt ctgccctttt
     4501 tcaaactttg ccattttctg ggttgatgag ccgacaacct gaaagatcac agtaataaga
     4561 cctgcgtctc tgtcgtattc agctacagtc agcggtattc gttccgcata atcatctgcc
     4621 cttagaacga caaattgtcc tgcttttact ttttttgcaa tatccggtac atatagctta
     4681 aaaagcgtca gagaaggtgc cagatctctt ttttcaacaa tttcattcag tttttgttct
     4741 cttgccatag tttctccttt ttaaaatttt tggggtcaat gtggtaatgg taatggccct
     4801 ctatagttct ttttttatag gtagtttcac tgtaaatgca gtaccctttc ctgcattact
     4861 gtccacctcg ataaacccga agtgggattc tacgattcgc ttcactatcg ataatccaag
     4921 acctgttcct gttatatacc ttgtctcagg cccgcagatc cgataaaact catcaaatac
     4981 gctcgttaag tgttctttgt caataccaat gcctgtgtcc tcgattttaa tactcaaata
     5041 atggtttgta ggaccggctg aaatcgcaac cttacccttt ttcacattgt acttgatagc
     5101 attcgaaata aggttcgtga agagttgttc catttcactg cgatctgctt caacaagagg
     5161 taatttttcc ggcaaattca aggaaaaagt gatttcattt gcctcaccct gattcctgag
     5221 cagttcaacc gtgttaatca gaatatctgt aagatccagc aattcctttt ttctttcaac
     5281 cttcttgctt tcgagcctgc tgaattggag gagatcattc acgaggtcca gcagtgagtg
     5341 ggtacgcgcc ttagcccttt caagcatctg acgattcatg tgagggtcat ttcctgcagc
     5401 accgcttaag tatgctgaaa gatacccctc aacagcagac aaaggggctc tcaactcatg
     5461 ggttaccatg gacacaaact gcgattttaa ttggtcaagc tccttaaggg cggtaatatc
     5521 cctaaacgtg ctcacaacac ctaaatcctg gccatccttg tccctaataa ctgatacgtt
     5581 tgccatcaat atccgcttgt caggattggc aagttcaatc tcctctgaaa ttgcagtata
     5641 ttgagaggag tccggtgaaa gggccttatt gatgagattt attaaagtgc ccacctggat
     5701 agtctcccct atatctttgc cggcttcaag tttcttatca agatttagca ttttcactgc
     5761 tgctggattc catagaacca gctggtgctc tttgttcgtt acaatgatac cgtcagccat
     5821 agaattcaca atagcatgga tctttgactc ctcgtttgcc acttccagga gcttctggtc
     5881 tctttcttcc ataagcttct gggcgtgaag ggttagacgc ctcttttcaa gacccctgtt
     5941 cacaacagca agaagctgat caggagtaaa cggcttcggt acataatcat aggctccgtg
     6001 tttcattgcc tccacagcag tttcgatcgt ggcaaacccg gtgatgacaa tcattatgat
     6061 ttcggggtca tgctgtcgta cctgctccat catctcaagg ccactcatga caggcatcat
     6121 gaggtccact aatataagat caaaaggcct ggccttagtc gatttgagtc cttcctcacc
     6181 attctccgca acgtcaacgg agaagccttc actcataaga attcgccggc agccttcccg
     6241 gataccccgt tcatcgtcaa ctaccagtat gcttgctgtt tccgccacag ttaacgccta
     6301 tttaacactc attcttttgc agactttgcg agaagttcat tgatcttttt tacaagatct
     6361 tcaggcaaca atggtttgtt ggcatagtca tctgtcttca tccagtaacc gtcaagctcc
     6421 tgagagaact ccattcccgt ttcactcccc actgcggtga gcattaggat aggaatattt
     6481 ttagtggtgg gatctgcttt catagctttc gcaaaaccaa agcctgagtc atgtttctcc
     6541 atcatgagat cgagaacgat caaatccggt tgctcgaatc tgactcgatc cagaccctct
     6601 gcgcttgaat acgcaacgac gacatcgaat ccgctgtttt caagaacagc cttgttcagg
     6661 tcaataaaat caacatcgtt gtcaacaagt aagatttttg tttttttgct catagttact
     6721 tctccctgtt attatatttt gaatccatgg atttttgcct ttatttcatc tagcacctgg
     6781 tcttcactgg ctttgactgg caaggtaata acaaaggttg ttccttttcc taattcggtc
     6841 tcaacatcta cttttccctg atgcctttct atgatgccat aggagattgc caagcctagc
     6901 ccggtcccct tttccttagt tgtaaaaaac ggatcgaaaa catgccgcat aacctcgggg
     6961 gggatcccgg ggcctgtatc ctgaattctg atctttatat gtttttttcc cgaggaaaca
     7021 ttgatcagta gttttccgcc gccctccatg gcttgggcag cgttcatgat gatattcaga
     7081 aagacttgtt ttagctgcgc ttcatccgcg acaatcgtct gaagacccgg atcaaatgat
     7141 ttctcgattt tgatattgcg aaacaagggt tggttgataa caaggcttag aacctcctca
     7201 agaatgtcac tgacctttat ctctgtggct tttagttttt tctctctggc aaaactcaaa
     7261 aggccctgga ctatatcttt tgccctggcc gcttcactga ttatcaagtc aacgtcttca
     7321 cgtctcggat catcagattc aagacttttc tggaggaggt gggcatagat ggtgatagtg
     7381 cccaagggat tattgatctc gtgggcaacg cctgctgcca gttgtcctat agaagcaagt
     7441 ttttcggttc taatcaattg cgcttgtgcc tgctcaagct cctgatgtga tatctgaagt
     7501 tctcgatgag atttctcgag ttgttgatag atcttcttgc tttcgccaag caaatgaggc
     7561 aggcaatgct cttcaggaga caacccctga acaactgcga ttgccttgtc tctgcaggtc
     7621 ggatagccac aggccctgca atcaaggttt cggttcgggg gaagcttgcc aattctcttg
     7681 agtactgccc tgatttcttc ctctgtcgga gagggcagcc tttgttccat gttttgaaag
     7741 ctccgaccaa gatctatgtt cttgaacctg tcgagatctg ccatgacact cacaacatcc
     7801 tgttttgcca tttcagattt tgtgtattgg gctacaattt gccttcgtgc gaggattgaa
     7861 tggtcgcgtt gtgcaaatgg accatctacg cacccatagc aatacacgaa atcgagaaat
     7921 ttaccctcca tgtatccctc ggcgagttgc tttagcgatg aggtcgtccg cctcggccca
     7981 cctataacgc ttatctcatc aatgagaaca tcgaaacttg gcccaatatt gcggtacaag
     8041 cctcctatta ccggaattat tcgaccctgg tacggtcttg gcccatcaaa ctcggtttcc
     8101 ggcaacgacg ctttgtcaac tcctgcttca atgagaagat tccgtacatc aacgaatgta
     8161 ataacttcat caatggcgcc agccacctcc ggagcaacga tttcgcccat tcgggcaaga
     8221 caggaagtta gataaactac tttccaatcc gggcttttta gttcacgggc cacccttccg
     8281 atggcaatca tgggagaaac caccggcgcc agattcggta taagttgagg gtaatatttc
     8341 tgtatgtaaa aaaagattac agggcaaaag gaagagatga gtggacgaac agcctcctcg
     8401 tggagcatct taaagtagac ttgactcaca agctcggcac cgaaggcgcc ttcccagact
     8461 tcatcgaagc caatctgttt caaggcgctc acaaattgtc ttggcttacc aaaatcagtc
     8521 tccacgggaa agctcgggtc aaggactgct atggtcttct ttccctctac aaaaaactgc
     8581 tttactttgg ataaacctcc atgataggtg agggcacctt gagggcatgc ccttatacaa
     8641 cctccacaca aaacacaacg ttcctgaatg acctggaaac catctctccc caccttgatt
     8701 gcattcgcag gacagatttg gacacaataa gaacatctgc gacatttccg gttatttaca
     8761 tcgatatcat aaggattcag tcttttgtct ggctgtgtta ttgtcatcgg ttgtatcgta
     8821 agcgctcgta ttataccaaa attgttacaa ttttacactg aaatatggtc atgagtcaac
     8881 ggcggttatg gttccctttt actattacgt gaaaaaatga acaaataaaa gttgattggt
     8941 tcgataaaaa ccttttatac accaaggtct ttctctttat gcagtcaaaa tgtgatgtgt
     9001 gctcttgcca tataagaaaa agatggttat ataacccact aagagggtga tgacattgat
     9061 ggctattaaa gatgtcatga aagaggtcat gttaattgtg gaagccatcg aagaatctga
     9121 tctcttcaaa ggggtaagcc ctgaattcct taaagaactg gctcagattg gagagatgag
     9181 aatctttaaa cgtgattcaa tcatatgcag ggcaaacgaa aaagcccgtt acttttatca
     9241 gcttctcgag ggtcaaatcg acgtcatgtt atctgaaaaa gaaatcatcc actttacctt
     9301 aaacaggcca ggagaaatct tcggatggtc ctccctcgtc gagccgtata tgtatacagc
     9361 aactgcaaaa tgcaatactg atgtaagggt tgtgaggatg gcaagggagt ctcttgaaaa
     9421 aacaatgata aaatatccag cagatggatt ggctatttta aagcatctca ccagtataat
     9481 ctcacaacgt ctccgtcacg cgtatctcta tatttacagc aaagaattaa ataaaaataa
     9541 agtttctgaa aaataggtgc tcaggttcgg gaaaaacgag ggtttcaaat tcttagttga
     9601 aatctgtggc ttcctttttt tcatcatctt tgatcacttt ctcctaccac tacccgaacc
     9661 agagcttcgt tctgcaatgc cgtgtgtggg catgctttct ataggattga tggaattaag
     9721 agttttccgc aagggagagg tatctttaat ctatggagag tgaggatttg gtctgtggtt
     9781 tccatgatag aatctgcttc tgatgcattg tgatggcaga gaaccacttt ttgacatcaa
     9841 ggagtatctc ttttgcgtct gcctattcgt tttgttccat ggcagttata agtcactgat
     9901 gattcccttc ctgccatcag tgatccaaaa gatgttcttt gtcagaacaa ggtgtcgtct
     9961 ctccatatca acacagcgtt caatatagac ctcatggttc tccattcccc tgccggaacc
    10021 cgaaaacatg cttcatacca tccttatcaa aaccaaatgc agtcatatac gcctctcctc
    10081 cccgatgggt tgtgctgatg aagagagtga agatgatgac aaggtctttt cccatgacca
    10141 gagaaaagtt ttttgcagtg atgggatgtg gcaggaaatg gatgaaggaa gcttggtttt
    10201 ttgcggagca aaagttcaga agccaaacgc aagtaagcga agctgcggaa gcgaatgagt
    10261 gtgattgcga gaaatgctca acaccaatga tagccccata aatacttggg atatggagat
    10321 agatgaaata caatcaggtc atactgaaat attcagcatg acctgattgt atttcggttt
    10381 ttcagtttaa ttttactgtt ttcctgtggt gaggtactcg tggatggacc gagcagcttt
    10441 tcgtcctgct cccatggcga ggatgacagt tgccgcgcca gtcacgatgt caccaccagc
    10501 ccataccctt tcacgggttg ttttgccggt ttcaggatca gccacaagtg tcccacgcct
    10561 ggtcgtttca agcccaggtg ttgttgaagg aaccaatgga ttcgggctgt ttcctatggc
    10621 acacaccaca gcatccatct ccattttgaa gttagaattc ggaatctcca ctgggcttct
    10681 cctacctgaa gcatctggct ctccaagttc catcttcaag cactcgatct ccttcagcca
    10741 gccattctcg tccccaatat acctgacagg gagggtaagc atgttgaaaa tgactccttc
    10801 ttcctctgca ttttcagctt cctctgctct ggcagggagc tcagcatgag accgcctgta
    10861 tatgatccgt acctcttccg cacccatacg aagagctgtc cgggcagagt ccatagctac
    10921 atttccacca ccaaccacag ctaccttttt atgacgtttt attggcgttc ttgatgccgg
    10981 atactggtac ccctgcatca aattcattcg tgttaggtat tcatttgcgg aatagacacc
    11041 attcaggttt tctccgggaa tattcataaa ccacggcaat cctgcgccgg tgcctataaa
    11101 tatggcgtca tacctatcga gaagttcgtc gatagagcgt gtcttaccta caacgtagtc
    11161 cgttatcacc cgaacaccaa gctgtttcac atattctact tctcgggcta ctatggtttt
    11221 ggggagccgg aattctggaa taccataggt taaaacccca cctgccttgt ggagcgcctc
    11281 aaaaatagtc acttcatggc caagcagaac taaatcacct gcgacagtta ccccggcagg
    11341 ccctgacccg acaacagcaa cccttttccc tgttggcctt gcctttggcg gaaggtcgac
    11401 ttcatcctgt gcagcctccc aatcggcaag aaagcgctca aggcgtccta tggcaacctc
    11461 tgcccctttg ctcttcagta tacattgtga ttcacactga ctttcctgag ggcaaaccct
    11521 accacacact gcagggaggc aattcttctc tttgagtttt cgaattccac cccgaaaatc
    11581 tcttttgact atgcatgata taaaatctgg tatatcaatt tccactgggc acccagtctt
    11641 gcatcgcggc ttcttacact ggaggcagcg gctggcctct gcaacagcca attcttcagt
    11701 gtagccaaag gcaacctcat taaaattatg cctacgcatt tcaggcgtct gttttggcat
    11761 atcacgacga ttcgtatcta tcctgctctt tttttcctcg ctcatgagtg gcatttcccc
    11821 tctttgtgtt ctatgggttt cggttcactg tgccgaaaat aagatgtctc ttcatttata
    11881 tactgttttc tccgagccag aaattctacc caatcaactt catgtccgtc aaactcaggg
    11941 ccatcgacac atgcaaactt catcttgttt gaaacggtaa cgcgacaaac accacacata
    12001 ccagtcccat caatcatgat gggattcaga ttaacaaccg ttggaattcc aagattcttt
    12061 gtcacttttg atgtatgggc cataaggaat gtgcaaccat tcacaacaat ctggtcgggc
    12121 agactgggta gactgtgtat aatatcagtc aggtgtggca catgaccttt tataccgagg
    12181 cttccatcac gagtaatgtt gaaaattttg gcaccgaggc tcacatatcg gtctgcccaa
    12241 tacagcagat aagaacttct tgcttcaaaa accattataa tctgattccc agcatgccac
    12301 agttcgcgag ccaccgggaa aatacttcct attccataac atcctgccac acacatcact
    12361 gtcccatatt ttttaatttc agtagcattg ccaagaggac ctacacaggt gggtataaca
    12421 tctccagcct taagctcagc aagcgcacct gttgaagcgc caacttccat gaagatgatg
    12481 gtgatggacc cggtatcgag gtcccagtca gcaactgaca gagggattcg ctcaccttcc
    12541 tcatttaccc gaactatgac gaattgacct ggtcgaattt tgtgggcaat ctcaggcgcc
    12601 tctactgtaa ggaggtgcat attgggaact atcatggtac gttcgatgac tttattcatt
    12661 gtgcctcctt atctgagaaa gatgatgacc tgtcccaatc ctcaatgatc ttcatacctc
    12721 ccacaacctg ggcaaggggt atgccgcggt atgaggaata cgtagccgag ggaaggcttt
    12781 caggctcaac cttggtagac cctttcaacc atttcccccc attcttaaaa tcaatcttta
    12841 caaaatccac tttcttctta ctggctttaa aattaggtat ctgtttcttg atttcgttct
    12901 gtattgaatt tatatccttg tacttaaact tccccttttt catcctgtct gctattcttg
    12961 tcattatcca ccagtcagcc cttgaatcca tatactgacc agtggccttc tccagcgtga
    13021 ggattcttct ctccacgttg atagtagtgc cgggtgattc aggaaaaaga ctcgtgggga
    13081 ggatcaaatc tggctgccgc caagatgatg gcattatcgc attctgataa atcagatagt
    13141 cgcaatcagg tatgatatca aaacgaccct ctcctacaag gtatacaagt tttttccgtt
    13201 ttgtaggatc aagcgacggg agacccagct ttatactttt ggaaccattc tgcaaaagct
    13261 ctcctggttt aactccggga aatgcaccca tagcaagcat gccaataaga tttgtatatg
    13321 ggtgggcaac gataattttc catcccaggc gatccctgag aacctttata cgttcaaaca
    13381 cctcattacg cctgtatgaa aagattactt ctggccccat gatcaggacg tttgccgatg
    13441 aaccggagag cgcttcggta acctcattta aattacctgc aaattctttg gttcccctgt
    13501 tcttaccttt tcccttcctt gcaaggctgt ctgtgaatac atcaagaaaa ccggcccagc
    13561 gggatggttc catcttaaaa gatgcctccg agagcatgtc aagattagaa tcacaggacc
    13621 cgagtgtaac cagcacagca cctctttgag cagccctttt ggctgcaata ccaatgggag
    13681 tataaccata tgtggaatca aaacctacgg agagaatccc ctgagctgtt tcaagagaat
    13741 cataggtact cgtataaagg acaagatcga aaaaagatga aatatcattt cccaaatcga
    13801 ataacacgga cgacatcatc ggtggtgaac caagcacctg cctgacaaac cgttgtgcca
    13861 caaaaagatc ctcattcgat aactgcggag atatcacaac aagaatatcg tccggcttca
    13921 cgccggacag tttttctgcc gccaaatcaa aggcctgatc ccaggtaatg acttcgtatc
    13981 ctacgggtgt cagtttctgt ggcgttgtat tacgttgtgg gctatttaca tattccggga
    14041 tggcaaatct tcccttcacg cagataaggc cctgatcaac ggtatcatca tagtcaggca
    14101 atacatcaat tacttcatca ttttttactt gaagcaagag acggcatccc actgaacaga
    14161 aggtacaggt tgttcccacc tgagcatctg ctataccata ccatttactt gtctttgttg
    14221 agagagcccc tgtagggcat acagatacgc aagcaccgca gaactcacac cctgcctcca
    14281 ggtgggtacg gtcaaaagca ggtccaatcg ttgtcagctt gcctctttgc ttgaatgcca
    14341 gtgtgccatt gaaacgaatg ctgttgcata cgcgtataca acgtccacaa agaatacaga
    14401 ggttatagtc tctatcataa aagggatcgt tcttctcaac tggaaaacct ctgtaatata
    14461 ccggatagga tgtttctgta aggccgacct tgtcgacgat ttcctggatc tcacaccggg
    14521 catcattggg acagtaccgg caaccggtta ttacgccaac cttgcgtatg gtgcccatga
    14581 actttttgca ttcatcatgc tcgtcgcaaa acaagcagct tgctggatgc tcgctcagaa
    14641 gcagtttgag aatctcttgc ctcagtcctc tgatttcagc cgtgtcggtc cgaactacca
    14701 tgcctgcctc gactggggtg gtacatgagg taggaaaacc tctcagtccg tcaacttcta
    14761 ctatacatag acggcaagct ccgtaagaag gcaagtgttc cagggcacac aatgaaggga
    14821 tatagacatt gttgtctcgt gcagcctgca gtatcgttat attaggtcgc acctcaacct
    14881 gtttgttatc gatagtaatg gatatcttct tttcttttgt catgttcttt tctccgcgct
    14941 tcttatcacg atggcatcac cggcaatggc ttcaaaacga cagacttcat aacaggatcg
    15001 gcatttgata cacttggtgg ggtcaagatt gtgcggttca gaacgtggtc cgctgattgc
    15061 tcccgtggga cagaccctga cgcacgactg acagccagta cacttttctt taatgacacg
    15121 gtactcaatc agatccttac acacggttgc cctgcaccgg ccgtccttta tgtgctccag
    15181 atattcattc cgaaaatacc tgagcgtggt caggactgga tttggtgcag tctgaccaag
    15241 cccgcagagt gcccctcttt taattgtatc accaagggta cgaagggcca taagatcctc
    15301 tggcgtacca tccccatgag tgatcttatc gaggagctca accatatgtc ttgttcccac
    15361 ccggcagggc acacatttac cgcatgactc tttttgagta aaatcgagga aatatctcgc
    15421 cacatctacg atgcacgtat catcatccat gatgatcagg ccaccagagc ccatgataga
    15481 gcctacagat gccagggatt cataatcaac gggcagatcc aggaactctt ctgacaaaca
    15541 acctcccgat ggtcctcctg tttgaattgc tttaaagttc ttttgtaccc cccctccaat
    15601 atcaaagata atctcccgaa gaggcgtacc gagttgaacc tcaatcaggc ctggccgacg
    15661 caccttaccg acaagagaaa aggtctttgt tccgcgatta ccttcttttc cgaattttgt
    15721 gtaccattct gccccgttcc tgaggatatt cggtagtgtg ccaagggtct ccacgttatt
    15781 gataatggta ggacatccat agaggcctga tatggctgga aagggcggac gggatcgtgg
    15841 cattccccgt tttccttcta ttgacccaat gagcgctgtt tcttcgccac atacaaaggc
    15901 ccctgctccc tcttttatct tcacgtcaaa actaaagtca gaatcaagaa ttttatttcc
    15961 tagaagcccg tattccctca tctgccctat agcttttttg aggcgttcta tggccagagg
    16021 gtattctgcc ctgatgtata tgatcccttt actcgcccct atagcatagc cggcaatgag
    16081 catcccttcc aacagggcat gaggatccga ttcaatgagt gacctgttca tgaaagcacc
    16141 tggatcacct tcatcagcgt tgcagatgag gtatttctgc tcaccaggag aatttctgca
    16201 cacagtccac ttcttaaagg ttgggaaacc tgctccgccg cggccccgca aacctgcctg
    16261 ccgaaccaca ccaatgacat cttcgggaga cattttcaat gcatttatca aaccccggta
    16321 accatccaga gcaaggtact gatcgatgtc ttcagggtcc acaaaaccac agttacggag
    16381 aacaatccta acctgcggtt taagcatggg aagatcgaaa aaccttggga tgtcataaaa
    16441 agattcatta ccaaaattac ccagggcaag tttcgtgtga ggaacaccct gaagcaaatg
    16501 ggatttgaga attgcaggaa gcattttcat attgacgttg ttgtaactca cccggggttg
    16561 ctcaggaagc ttgatgtcaa ccaggggttc cagatagcaa ggacctatgc atccaacctg
    16621 aataaccctg gcaaaaacct tgttctcaac aagaaattgg tcgataccgt ccaggagttc
    16681 caatgccccg gctgcccttc cacaggacgc tgccccaaca taaataatgg gctctgtgtt
    16741 atcttccagg tccttccatt tttttttggc caatgatatt tttttattta tcttattcat
    16801 agtcttttag gtgctccgta agtttgttta ccgtcatcct gctgtatatc tctttatcta
    16861 tttgtacaac aggagacaag gcacagcagc ccaaacatgc aaccctctca agatcgaatc
    16921 gtttatcaga tgttgtttca ccgcaatgta tgcctaagct ccgttccagc atctcaagca
    16981 aggtcgcacc gcctcggaca tggcaggcag tgccaaggca gactttgagt gagtgacggc
    17041 caggttcggt aaatcgaaac tgcgcataaa atgacgaaac accatagatc tgattctctg
    17101 agatgcgaag atatcgggaa atttttttta ccgattcagg ggagatatat ccgtattctt
    17161 tttgaatatc ctgcagaacc ggaatcagct cttcctgttt cctgtcatgc tttgcgagaa
    17221 tagactcgat tggctcaggc atagtgttct cctttaaacc atgcattatg tggcagtcgt
    17281 tcgatgggta aaccagttaa tgctttttta cgccacagga ttgtgacggt taattgatga
    17341 gaaagaaaac aaatcgattc ttcctctttt atcacaaacc ccccattcac accattatta
    17401 ttaaatcgtc tattttatgt caagtacttt tttttcacaa gtcttatttg tatacttgat
    17461 ggattgtcag acgatgtgag tgtctgttta cagtgtgagt cttttatcaa ggacgcggta
    17521 ctgaatagcc tcggcaatat gagattcgga aacatggtcc tctttttcca tatctgcaat
    17581 agttctggca acacgtatga tcctgtggta tgcccgcggt gaaaggccaa acttttcaac
    17641 tgctttttca aggatattcc taccgttctc tgtcaggtgg cagtatttct ttatcatcct
    17701 tgcactcatc tggccatttg catagatttt tttttctgca aaccgttgtt cctgggtttg
    17761 tcgtgcctgt accaccctgt ctcgtatttc ataagatgac tcctcttcct tatcaagaga
    17821 aagttctctg atagtcactg gcggaacttc aacatggagg tctatcctgt caagaagagg
    17881 ccctgaaatt cttgatctgt agcgatgaat ctgggatcct gtgcaggtac aggatcgtct
    17941 cgaatctccc cagtaaccgc atggacacgg attcatggca gcaatcagca tgaagcgagc
    18001 cggaaaggta atggcatggg tgaccctaga aacagtcaca tcaccatctt caaggggttg
    18061 tcgcaacgct tcaagaacat tccttttaaa ttcggggaat tcatcgagga agagtacacc
    18121 attatgggca aggcttactt cgcctggttt tggcacatga ccccctccaa taaggccagc
    18181 atctgatata gtatggtggg gcgctctgaa tggtctattc aggacaagga acttgcttgc
    18241 acttaatagg ccagcaatgc tatggatttt tgtagtctct attgcttcat cataactgag
    18301 aggcggcatt atggttggaa tccttctcgc aagcatagtc ttgccagatc ctggtggtcc
    18361 gatcatcaag aggttgtgac ttccgcttac cgcaatttca atggccctct tcgtctgtgc
    18421 ctgccctttg atatcagaaa aattgagccc agttcctgtc ccgccatcct caccctgtgc
    18481 attcactgtc cggaattctt caagggattc gccaccttta aagaagtgga caatctcgag
    18541 aaggtgtttg gctccaaaaa tcttgatgtc ttgcacaacc gatgcttccc taccgttctc
    18601 atgtggtaca attatgtttg ctatcttctc cttacgggcc agtatcgctg tgggcaaaat
    18661 tccccgtacg ctttttatcc taccatcaag tgacaattcg cctgcaataa ggaaattgct
    18721 tatcgtatct ttttttatga tatttaaagc cgtaagaatt cccaatgcta tgggcaggtc
    18781 aaaagatgag ccttcttttc ttacgtccgc aggcgccaga tttattgtta tcctatcatt
    18841 gggaaattca aatcctgcat tcttgatagc agacctgacc ctttctttgc tttcctttac
    18901 agaggtttcc ggaaggccaa cgatattgaa ggcaggaaga cctaaagaaa tatccacctc
    18961 cacgtcaatc tttatgccat caatgccata aatagttgca gtgattattt ttgaaatcat
    19021 atcccccccg tctaatgcaa gaaaccaagc agttgaggag ttcagaaggt atggaaaatc
    19081 aacttttcag cctctgcttc tcagtttctc aatttctcaa cttctcattt gctatccttc
    19141 ctctcccccg caagccgggc tatctcacga acttcctgtg cccgttctgc gttacacaca
    19201 agaaccccgt cacatgatgc aacaatcacc acatttgaca accctataac accaaccttt
    19261 aacccttcgc tgcaaacaag agaattcctc gcatcaatac aaacagtatc gcccgagcag
    19321 taattgccgt ttaggtccaa ctcctttaac ctcaaaagtg acgaccatgt tcccacatca
    19381 tcccaagtga attcaccagg caccataacc acgttctggg ctttttccat caagccatag
    19441 tcgatcgatt ttcgaacaag gttagaatat atttcaacaa aaagttcctc gtttctatcg
    19501 gctttatatg tctgtatggc aagtagacct ttatagaggt ccggcatatg ttcagcaatc
    19561 cctgcaagca cggttttcac tttccagata aacatcccgg cattccagaa ataattgcct
    19621 ttttcaagat atagctgcgc atgttcagca tctggtttct cgacaaattt tgttactgcg
    19681 taacaggcct gatgatttga tgaaaaaatt atttcaccgg ctttaatata tccataacca
    19741 gtttctggtc tgtcaggttt tatcccaatt gtgacaaggt aatcaccctt gcttgcaata
    19801 tctgtcccat ataagacagt cttatgaaag ctttccgtat caggaataaa atgatctgct
    19861 ggcagcacaa tcatgattgc ctgtgggtct atttcaagca gtgaaatcgc tgcaaagcca
    19921 atacaagggg ctgtgtctct tccctcaggt tcaacaagaa aattattgag gggcaaatca
    19981 ggtagttggt ctctcgcgat gtccacatgt gacttcccaa ggacaacata aatgtgatcc
    20041 aggggaacta gttttgctac gcgttccact gtaagctgaa tcatcgtttt agcaccgagt
    20101 attttcagga aaggttttgg catattgtgg gtgttcaagg gccagaacct ctcccctctg
    20161 ccaccagcca taatgataca ataggtatga tcgagaacgc ttatgtcagc tgcctggtgc
    20221 ggggtaacag atgaattacg tttcatggtt ttgctatgag cttacagcta ttagctataa
    20281 gctttattct aaatattcga gaatctttgg taatatttcc gatgcgctgc ctttcaaaaa
    20341 atggtctgtt atggatgagg taaatggagt ctcttcaaca ttgatctcga tgatcgttgc
    20401 tccatttgac ttggcagtga aaggaattcc ggcagcagga tagacaacac cggatgtgcc
    20461 aataataaac agggtattgc aaacattcgc ttcttcattt gccctgaatg ctgcaatcat
    20521 ggggatacct tcgccaaaaa atacaacatc aggcttcagt gccatattac atttctcaca
    20581 tcttggatat gggtaaacac tgactacttc acgggcaagc ggtatttttt ttgaacagtt
    20641 cggacagata agccacctgt gattaccgtg atattcgatc acatccgtgt tgcctgccac
    20701 ttggtggaga ccatcaacat tctgcgtaat tacagtcttg aggatgccct ttttttctaa
    20761 tgttccaaga gcaaggtgtg cagggcttgg cctagaatca aaaattacct ccgacatctc
    20821 cctgagcatt acccacacct tttccggatt atgattgaat gcccgtatct cggcatattc
    20881 catcggatcg tatttctccc ataatccttg acctcctctg aaagcaggga ttccactatc
    20941 aacagatatc ccggcaccag taaacgctac catacgccct ttttctttga taatttttgc
    21001 tattgccttg taatcgtcca taaattcatt tttctcgcac ttttttcttt tttcatttta
    21061 atcgattgta tgtttacacc ttctcctcga tattctctct accattacat gaataaccga
    21121 taaccgttca cagtatttca tcacgttgtt ttccaaccct ttgtgatatc tctaaaggct
    21181 ggaacaatac cgaaaccatc ggcttttttc acagctctct taatggcttc agcaataaca
    21241 aactctccta acacgccaac aagattgata tccgcctcgt atacgcctgt tgcaagtgcg
    21301 aagacgaggt cgccatcaaa tgtggtatga acagggttta tggtcttgat aagccctcct
    21361 tgtgctatct gtgcaacctt tgtaatctct tttttgttaa atttagcgtt cgtggcaaca
    21421 acaccaaggg ttgtgttcac aaggccaaac tgttttttga cagcaccttt cttaatactc
    21481 tcaacagtat tcgcaaactc aaggctatca ggggcaaccc tggcacctgc tataatcttg
    21541 cctgtcacgt tatcgattat gtctccgaag gcattgacca ctacaagact ggctactacc
    21601 aggccatccg gcattacaac actgcttgtt ccaactcccc ccttcatagc cctggatagt
    21661 tcgaatatct ttcctactgc tgcaccagta ccaacaccca cgctcccttc ttcgactgta
    21721 tcccctgcat tcatacacgc ctcatatcct aattccagag ttggcctagc ctttgggtca
    21781 ccgaaaagta tatcaaaaat aacagccgtg gggacgattg gtatttttgt tggcccaaca
    21841 tcgaaaccta cccccttttc ctcgaggtaa cggctgacac cggtggcggc atctaggcca
    21901 aaagaactac cccctgacag gagaattgca tgaacatgtt caacgatgtg attgattccc
    21961 aaggcatcaa cctgacgggt acctgatgca ctcccccgta tatcaatacc acatgtggcg
    22021 ccatcttcac agaggatcac cgtacatcca gtataaccct taaagtctga ggcatggcca
    22081 acctttatcc ccttaacatc agtaatcgag ttgagcatag ttacacctta tcaaaacgtt
    22141 cttttattgt caatcttcta gtatatcttt taatttctta gacttatctt cgttgtattt
    22201 tttcatgtct gacttcattt tatcaagctt ctttctcact tcatcatatt ttattgacaa
    22261 tattttgcat gccaggatgg cagcattcct cgaagcatca atccccactg tggccacagg
    22321 gatacccttc ggcatttgga caatcgacag aagggcatca atgccattca gaactcctcc
    22381 acttgtgggg acgccaataa caggaatagt agtgtgtgat gccaccacgc ctggcaaatg
    22441 ggcagccatg cctgcaactg cgataatgac ctcaatacct tctgttcgtg ctcgttgtgc
    22501 atagttgatt gttttttcag gctggcggtg cgctgaggat atatcaagcc taaacggtat
    22561 cccaacttct tttaaaaact ccaaaccatc ctttatgaca ggaaggtcgc tgtcgcttcc
    22621 tataaggacg aggatcttgg gcttttccat gaattctcct tgccatgttt tttcacagac
    22681 cataatcata caaaaaaagg agtatttttt ccataaatat aagaatgagc actgaactgt
    22741 aaagctatag ctgtaagttt tggtgcaggc acctatcctg cttgaaggca agaagaaaga
    22801 tggccgagcg gaaactagac aagaattcgg atattatctg tcaatggcaa gcttgcctgc
    22861 aatagaataa tcatatctgg ccatatcctg aagaataatt ctcacatcct ccggctttac
    22921 accgatgctt ttcactacag catccgtcat ttctgcaaca agcttccttt tctgatctac
    22981 tgttcttcct tcgaacatat gaacctcaat aataggcatg gttcctcctt tttaaatagt
    23041 cttggttatg tacccgggag ttaccattat gaagcactat gcagtcccgg gatttttgaa
    23101 atactgtgaa aaaacactgg tatggtatga ccgatgcaca gacacaaacc ctgtagatca
    23161 ttctgtatat ttgcccattg ggctgataat gtcaatactt cgtttattta tccttctcaa
    23221 tgtttttacc ttcatccttt cctctttaca aataacttcc ctgtcaatcg ttatctaggc
    23281 ctgttagatc tgttgaagca tagcattccc cggtggaatc tcgactaatt ataaggctga
    23341 aagctaatca tgattgtttt tccttcattc tgtgaatttc gcaatagact tcatgtacat
    23401 atcgagaccg attatcataa tgtcgttgtg gcctgcatgt gggatataga gcgagtattt
    23461 ctcttgtgat cccgagaggt cgtaaagcat cctcccttct gttaccggga taatatcatc
    23521 ctcgtccccg tggatgataa gcgttggaat agtcacttct ttgattttca aatgattccc
    23581 aaaacctatt ggatcctgaa catctctgaa aagatgggac attcccaatc gcataagctg
    23641 attgcggcct gatgcaaaac cgctttcgat aatcaacccg ttcaactgct gctggtaatg
    23701 ataagccact tcaatggcag gggcgcttcc caatgatcgc cccataacga aaagattccc
    23761 tgagaaattc atgtcatgaa gaaaatcaat aaaactgtga aaaatacaat gggcatcacg
    23821 gatcatggac ccagaactcg ggctgccatc actcatcccg taaccccggt aatctgcaac
    23881 aaacaggttg attgctcttt tctgatagag tggcgcaata tagtcataat caaacaccgt
    23941 ctcaccatta ccgtggaaat aaagaatatt tggtccgtta ctttgtgcag gatagaagcg
    24001 gcaaccaatg gttatgccat catccatttc aacaaaatag ttttttgctt ttagatgatt
    24061 cggttcccct gaaaattcgc gccgtggaaa aaagagccga ctgacaatct ctggatgatc
    24121 ccacctctcc aatcccttat caaaatcaat catggttatc ctctccctga agtatttgac
    24181 cggttgtata tattcgttgt tcagtcagtt ccagattcct attcagtaac atattcatta
    24241 ccggcatatc aaatcagaaa tttagttccc aaacgccatt ggcagggggt aatggctttc
    24301 ccaacgagac cagtctcggg caggaaaacc acatcttttc aacaaggaga tattctgaca
    24361 gttgacacga gcgtatacct gtctgggcag gagccaaccc ctcttcttaa gccatgactg
    24421 tggttagtac aatatgggtt taaacccatg aacaaactgt ggggttctct gcaaaaacgt
    24481 ttatttgatg gaaaatatca gttaagaata acgatagatt tatcggttgg aagggtagta
    24541 tctgtgtgta aagaagctca atatattgag cgaattctgc ctgttctttt atcaataatt
    24601 gctttatcta tgaggaagcc atcagaatca agcaattcag ctatgaagaa ccaccttctt
    24661 tcttctaatt tgccaatacc tacctccttg ccgattccct taaagtattg ttcaagtgca
    24721 tgccttgcct cgtttactgt tcttatctgt cttctggcac cgtaggggtc accgcgcatg
    24781 ccaggacaaa actgcccgta aggtgctctt tcgtatttat catacatccc tctgcgccag
    24841 ggttgtgcag aggaatgtat gggtatagca aagacaaaaa agattgctac aagcaaaacc
    24901 agaaatcgca tagatgttct cctcttgcag gcaggattct taattgtttg aggatcccgc
    24961 ctgcaagatt ttttttacca gttcgggcag taacccctac catagccagg cccatggccg
    25021 ccgccccagc ccatatgatg cccccgtcca taacctgggc catagctacc acgctttgaa
    25081 aacccgaagc caaaacccct gtcgggcaag ccattcttgc tggctgcctc cctaattttc
    25141 gtactgagat caatcatctc tttctgaaga gtggctatcc tcgcctgatc tggggtttct
    25201 ttaataaact cgttccgcag ttcaagcctt ttcacagcca tttcgtctct aagggacaat
    25261 gtttcctttt gaaagttcct gaaggcgttc acatccacct gtacacctgg tccgggactc
    25321 tgacccttag tccatgctgc aaatgatgct gaactcataa gagtaccaaa aacaactgcc
    25381 aaagcaactg ctaatacctt tttcatctct ttttcctcct tttttgagat atgttacgta
    25441 ttaatataaa ggaagaatgt gaaagattga cgaattcatt gtgaagaatt gtgaacacct
    25501 cctgtcggca aaactatgct gaaccttgat ccttttccta gaatgctttt agcctctact
    25561 ttgcccccat gagcttctac gagttctttc acaatagtca ggccaattcc cagcccgcct
    25621 cctgaaccat gatagaacct gtcaaagata aaagggaggt ccttttctgc tatcccggtt
    25681 ccagtatcgt caacggtgat tttcaatccc atgggcccgg acgacacatt cagtgatacg
    25741 aggcctcccg aatccattgc ccttgatgca ttatttatga gattcattat gatctggctc
    25801 agccgttcag gatccgcctc aacttcaaaa tcttcagggc attgaacttc tacatttatc
    25861 cctttttcca agaaaatatt cctgtaacgt tctgtaatat tctcaagaaa tggcttgacc
    25921 ttaaatcgtt gtttttgtaa ggaaagtatg cttgcttctg cctgattgag atcttcgata
    25981 ccgtccacta tcttttttaa acgccctgtt tcatcatgga gagattggag gtggtctatg
    26041 tcatttggaa tgagtccgtc tatcatgcct tccagttcac cgcgcatcac tcctaacgga
    26101 gttcttaatt catgagccac atcagctatc agcttcttcc tcagaatctc ctgcttctcg
    26161 agggcttgcg ccatatggct gaaggtatag gaaagttcgc ccacttcatc gtgccttgtt
    26221 atcgacaccc tttttctaaa atctcctttg ctgattgcgg atgcggcccg ggcaagctcc
    26281 ttgatagggc tagtcagccg ttttgaaaaa agaacgctta gaatgattgc aatcccacca
    26341 atgataaata tggccataag caaaaagcga ttggatcggc tcaaaaatat tccttcccta
    26401 agaggcctca gttctctggc ttcaagggta ccgatctgct caccggaaag gaataaggga
    26461 tagggaaaaa attcgccggg ctcttttaat tctct
//
LOCUS       DAOOMC010000023        26136 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdus sp. isolate AMR_MDS_2685
            NODE_1721_length_26136_cov_6.166711, whole genome shotgun sequence.
ACCESSION   DAOOMC010000023 DAOOMC010000000
VERSION     DAOOMC010000023.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864760
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdus sp. (wastewater metagenome)
  ORGANISM  Syntrophorhabdus sp.
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae; Syntrophorhabdus.
REFERENCE   1  (bases 1 to 26136)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 26136)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,729
            Genes (coding)                    :: 2,708
            CDSs (with protein)               :: 2,708
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 7 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26136
                     /organism="Syntrophorhabdus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1721_length_26136_cov_6.166711"
                     /isolate="AMR_MDS_2685"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2699756"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..320
                     /locus_tag="PK125_03800"
     CDS             <1..320
                     /locus_tag="PK125_03800"
                     /inference="COORDINATES: protein motif:HMM:NF015684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HPB37265.1"
                     /translation="YIRRLKRDGHDVRRYLVDLYNKTAFPFINLIMVFVAFSVGLRYS
                     KTKHIAKGILIGVCVGILYWFIHNICLSFGYAETFPPLFAAWLSNMLFFFVGIIGVVT
                     LRT"
     gene            435..929
                     /locus_tag="PK125_03805"
     CDS             435..929
                     /locus_tag="PK125_03805"
                     /inference="COORDINATES: protein motif:HMM:NF024873.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H-like domain-containing protein"
                     /protein_id="HPB37266.1"
                     /translation="MKAYLDIETDREGNISVIGISIGSNGFKQFVGKAITSNKVENCL
                     RQAKTIVTFNGDSFDLPQIKKQLNLDLAVSHRSYDLFKVKKWLNIKGGLKELEKMYGI
                     ERKTEGIDGFKAVQLWEIYRRHGRKDALELLLEYNKEDVLNLIPLEEKFNTLIKEQFH
                     GQKV"
     gene            913..1626
                     /locus_tag="PK125_03810"
     CDS             913..1626
                     /locus_tag="PK125_03810"
                     /inference="COORDINATES: protein motif:HMM:NF012546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HPB37267.1"
                     /translation="MVKKFEVSSAHNKIAGLMIIPPGRKKHPCVILSHGLVSSKESSK
                     YALVSEVFAEAGIATCRFDYHGCGESGGDITETTLTIRLDNLDVITEYIINHPSIDPA
                     NIGILGSSFGGTACVLKAAKDKRIKCISPWATPYRLDKDGDGTISGIPFKETIFTDFQ
                     KYDILFEARKVSDALVIHGEMDEVVPCEEGKTIYKNMKKPKKCVIIKNGDHVLSDPIH
                     RDRAIKLGLNWFQGYFLKG"
     gene            complement(1805..2482)
                     /locus_tag="PK125_03815"
     CDS             complement(1805..2482)
                     /locus_tag="PK125_03815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB37268.1"
                     /translation="MIDFFKDMFLDLYDNFTEVFLNIIVMIIVMLSGFIISWFIKKLL
                     ARLLVLFRFDKWANEVGIINFLQKGGVHTLPSIVISKIVYWIFIVVFFSFGLNFIGIS
                     QFTEYASRVSSALPLIVVSIVIIIIGIILSNFISRVIYLACENANIKYGEPIGRGVKI
                     LLVIITFGIVFEYIGVGNTIITISFLIIFGGIIMTLSLALGIGLSNVVGDLIKDRLGI
                     KTDKKKE"
     gene            2704..2877
                     /locus_tag="PK125_03820"
     CDS             2704..2877
                     /locus_tag="PK125_03820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016292899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HPB37269.1"
                     /translation="MPAKVDEDACTGCGACAEVCPVDAITVDDTAKVDPETCTECGTC
                     VDECPVEAISMEE"
     gene            complement(2948..4957)
                     /locus_tag="PK125_03825"
     CDS             complement(2948..4957)
                     /locus_tag="PK125_03825"
                     /inference="COORDINATES: protein motif:HMM:NF014695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heterodisulfide reductase-related iron-sulfur
                     binding cluster"
                     /protein_id="HPB37270.1"
                     /translation="MMEISREIFWNVKSTGEWIAYSLMVISFIILFLGLKKRYNMWKI
                     GKSEPFDFMKLLGQRIGYFIKSGVFHKTILRKGEGFPGWMHLFIFWGFLILAIGAFLD
                     AFQHHFTHLVFGWEYLKGDFYLWFSFFLELAGLAAIIGVLMAMYRRYVTKPERINDNK
                     PDDFICLIWILVVLVSGFLVEAARIAATKPDFEVWSFVGWFLAGLFSGMDKASIETTH
                     LILWYFHMVISFGLIVYIAYSTRLMHILTSSLNMMFRGVEDKPRGAIAPIPDFENAEE
                     FGVNNIEGFTWRQIFDLDACTRCGRCQDRCPAHLTQKPLSPKKFIQDLKGEWEKTAAG
                     IKNEDGLLDNVIQEETVWSCTGCLSCQVNCPVSIPTFDKNIEMRRYLIMTLSKVNSEL
                     KLLYKNLQTRFDPYGMGKSQRLEWTEGLDIKKATEEEVEYLYWVGCVASLDDRNRKIA
                     RSVATVLQKAGVSFGVLGPEEKCCGDPLRRTGNEYQYFELAEGNVELLKELGVKKIVT
                     ACPHCYNTLKNDYAQLGANFEVFHHTELIAKLAQEGKIKINANLEGITTYHDPCYLGR
                     VNQVFDAPRSVVNQFKKGSYVEMGRHHDEGFCCGGGGGRMWMEEHHLRMNHLRIDEAI
                     AINANTVVTACPYCLTMMEDAIKDREKTETMKALDISELVVKSID"
     gene            5198..6163
                     /locus_tag="PK125_03830"
     CDS             5198..6163
                     /locus_tag="PK125_03830"
                     /inference="COORDINATES: protein motif:HMM:NF013073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HPB37271.1"
                     /translation="MKRTVFTFVICCFFTFISVNLALSSNVTYKVKSGDTLQKISKKF
                     HVPVEKLKNLNGLSSTKLKKGQTLVIKKDVPARKERETASAPPAGKDSPVDLTTAEND
                     GEFIEYRPKKGDTLAKIASKFNVDKEDLLESNNITGRRLPKVILVPKIIAVDDSEDIV
                     DLSCGKPLKPWRNNDEKYMLVKVAKGFMGAPYKYGGETVRGLDCSAFVKKIYDIFDVQ
                     LPRSAREQFSVGHKIAKDQLTVGDLVFFKTKRYIKYPTHVGIYIGDGNFIHASSNRNR
                     LGVKVDALSSDFFSRTFMGATRVKKSPDDTAETSKNSQNQPSNNS"
     gene            6167..7357
                     /locus_tag="PK125_03835"
     CDS             6167..7357
                     /locus_tag="PK125_03835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB37272.1"
                     /translation="MNKKTTPILLFSILIHLSGYYVLIERIEPFQYFAYLILWWSYII
                     FIDAVLARKIKRFLILNKNLPFLIIISSGYWCLYEIINLRLENWFYINLPHNFFQRWA
                     GYMLAYGTVIPALYVTKELLLEILGEIQVKPRPMRAYQKLAVYAGIFALIMTIVFPIY
                     FFPLAWVFLILIMEGCNYQKGYSSFMREIEQGLLGNLIATILSGLVCGILWEIWNYWS
                     ISKWVYTVPFFEDLKIFEMPLPGYFGFLFFAVGTIAFMNFLQGIKVYSLYLLRVTSVA
                     LALSLFSFILIERNTVFSYTTRLDQLSFIAKEKLDTLKRAGVQISFGINPQQLDPMEK
                     DALDLIHLKGLGYDHFLKLRDRGIDTIQKLSLTDENAISSILDEANMRRIRVYLKAAK
                     KARY"
     gene            7611..8075
                     /locus_tag="PK125_03840"
     CDS             7611..8075
                     /locus_tag="PK125_03840"
                     /inference="COORDINATES: protein motif:HMM:NF021492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPB37273.1"
                     /translation="MAKKQTKDLIRKPDFLLQFIENAYIFIQENLRGFIIGAVIFVLA
                     VASVYGYAVYARKQEEKSQTTLFQGIKSFEEYSQGGKQESLTNAENVFQTLIKEKKGK
                     AYKIARLYLATIYTVQGKSDDAKMLYQQVIKDSSGTILQTLAEQALQGLEKK"
     gene            complement(8237..9460)
                     /locus_tag="PK125_03845"
     CDS             complement(8237..9460)
                     /locus_tag="PK125_03845"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPB37274.1"
                     /translation="MKYTLAFYLRIAFQNLIVHKGRMILALLGILFAVMSLVAFGNIS
                     NGLKKQIDSEIGKFGKNLIILRSGYMRFTGRGAMPFGDSKNLKLETVQRIGESVPGIV
                     QIAPFYDVSYPARYEEKTVTAGVVGTTEAMFQIRNLDLLMGTYFTEDDNVNGRKKAVV
                     GYKVFENLFQQENPIGKYILVYRVPTEVIGVLKEKGTDLAGQDQDLQVYVPLTSIMRR
                     YSNVDFIKGAYIQIQDKIPLTDMKQILQSFIRTIRNMKPEQKDDFSVFTMEDIVKTQE
                     QGIRLVSILTVIASIVSFLIGGLGIFAIMLLSISERKLEIGIRRVVGSKKRDIILQFL
                     TESVVVALMGGALGILVGFIITVFVSYFGNVPFSLYLENIVVSLLISTLVGILAGIYP
                     AVQGTKYEPVAVLYG"
     gene            complement(9457..10680)
                     /locus_tag="PK125_03850"
     CDS             complement(9457..10680)
                     /locus_tag="PK125_03850"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPB37275.1"
                     /translation="MGLFQKISRGREFLQEILKILYYYRGRVIFSFAGVALGILSICV
                     IITTIDGANKKAKDIFEVLGPDSIMVWGGGERQRTARMRGSTLTLADAESLDSIEGVY
                     DSVKTYYARRPTMKYKDRKWQSRIIGSTTNYFESMSWDLQAGSPFSTDDYKYARAVTV
                     IGSKVYDELFQGEDAIGKVILAGRLPMTVVGVLKERGGGTGGAQIDDRVIMPLTTVMS
                     RVVNDKKYLSGVRLKTNRDVNETVADVKAVLRRNHGLQGSAEDDFTIRSSQDIMKFVT
                     VISGQLLLFLGMASIVALVVSGFVLANLFYLTIQERRKDIGIRRAYGASRRGILLSFL
                     LESVIITLLGGIAGILLSLALGGTFETLFDIPMLFSYKVVVFSLMFSFLTGLIAGFRP
                     ALKASRIEPIEAIRG"
     gene            complement(10649..11323)
                     /locus_tag="PK125_03855"
     CDS             complement(10649..11323)
                     /locus_tag="PK125_03855"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPB37276.1"
                     /translation="MITLRNIKKSYFIGERELPILKGIDLEIRSGEFVVLMGVSGSGK
                     TTLMNIVGLLDKQTEGEYRFMDVFINGLDDERLASMRNLHIGFVFQQFFLLPYLNAIE
                     NVLIPIVYSSKDIKHPREKSKALLSRFGLAERVYNKPSQLSGGEQQRVAIARALINEP
                     DLILADEPTGALDSKTGGEIMDLFRILNDEGKTVIVVTHDPKLASFGKRLIRIEDGTI
                     SEDITR"
     gene            complement(11320..12456)
                     /locus_tag="PK125_03860"
     CDS             complement(11320..12456)
                     /locus_tag="PK125_03860"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HPB37277.1"
                     /translation="MKKKYVVIAAIILIAGGVLVFLFSGNKASQGKAGQDVTVKRGNV
                     THFTEQTGIIKAQVGAIVKVGTRATGTLTYLKYQVGDAVKKGELIATIDDREILANIR
                     NTEATIESLKKDLEAKEAQYLYSKTNYERELSLLEKEFTTRDSVDKAKREMDVALAQV
                     EYGTAKIKESAERRKALEVSLSYTKIYAPISGYVSAVSTQLGETVVSGLSAAILITII
                     DPSKLEMWIYVDETDIGRTTPGRKIEYWVDTFRDKRFYGKIDMIYPQPEIKDNIVYYL
                     AIVKIAPEDTAFLRPEMTTHVRIIVEEKTDVLVIPNNAVRFEEGKTIVYLKGRDKTEA
                     RQVTLGIRDDRFTEVVKGLEEGETIVVPVVVRKPSQNASPGSKK"
     gene            complement(12459..13757)
                     /locus_tag="PK125_03865"
     CDS             complement(12459..13757)
                     /locus_tag="PK125_03865"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HPB37278.1"
                     /translation="MFLSRRRIIFVCIMIVSFLSREACAITLDEAIKRALEISFLIKE
                     QKEIVKKTEYSYISTMDPYLPQVELYSSYVRSLNGKRSVSFLSGSSTSGASGSRDAYT
                     LNGSISYLLFDGGERYARRKGAFFSTLREKERLQGVKNDVLYLVKAAFYTVMGNKSIV
                     EKRQEAFNVAERVLALTKGRYSAGVAKKSDVLQSEVRMTTARIEYMDAQREYEKTLAE
                     FKSLLLYDPEDRQEVDGPFMEPQYKGDYQQLTERAINIRPDVTVQKNEIERLNMAYRE
                     KMSAWFPRIKTQLEQTRQDYQFFPEGRQETFRINFSWPLFDGIGRYYNMLGASRDIAA
                     AKYRLEEIKRTVSVEIIKALKDYELSAQNVTLYGELVREATSNFDQAFGEYKVGKGDI
                     MALLQSERDLAKAKENLVSAISRANTNLASLERVAYIGRE"
     gene            14222..14908
                     /locus_tag="PK125_03870"
     CDS             14222..14908
                     /locus_tag="PK125_03870"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HPB37279.1"
                     /translation="MKRLLFLIIFFSMMCIAPSIGHAQKPHYGYPHGYWRGSYYRPHH
                     RPYYYPWYGSYYRLLPRHYYAPAPAYVYPPHPVIYAPPPAVIIVPNQAYAYPDIPPPP
                     PAPSSQVTSPLQSVPTAILQSIYFDLNKSAIRPDAEKILKQNLEWFRQNPGRKVSIQG
                     NCDPRATELYNLSLGKRRAEAARQYLVELGVDTKLLDVVSFGKEMSTCKGKDESCWAK
                     ERRVDFIPSP"
     gene            15067..15594
                     /locus_tag="PK125_03875"
     CDS             15067..15594
                     /locus_tag="PK125_03875"
                     /inference="COORDINATES: protein motif:HMM:NF012774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HPB37280.1"
                     /translation="MKSLLRTKVFLIVALVLLCLLLILTLYADETAVVTDIVDGDTLA
                     IEYNNRKELINLIGIDAPESVMNRKAEIDSRTTGESLLTITSKGIDAKRFVERIVNKG
                     DTVFIAFDAQAKDQEGKLQGYVYLSDGRLLNEEVVRAGYAKVTASFSNPKFRDRFMEA
                     YREARTFRRGLWRSQ"
     gene            15825..16031
                     /locus_tag="PK125_03880"
     CDS             15825..16031
                     /locus_tag="PK125_03880"
                     /inference="COORDINATES: protein motif:HMM:NF018961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dodecin family protein"
                     /protein_id="HPB37281.1"
                     /translation="MSVYVVNEIVGTSTKSWEDATKQAIETAAKTVSDLRIGEVVKQD
                     VTVENGKVSSYRVRLNISFKYKEK"
     gene            complement(16135..16665)
                     /locus_tag="PK125_03885"
     CDS             complement(16135..16665)
                     /locus_tag="PK125_03885"
                     /inference="COORDINATES: protein motif:HMM:NF039354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6125 family protein"
                     /protein_id="HPB37282.1"
                     /translation="MKLPQFRNEELLPYIEFMVNQFRRTDGFWFIGVEQAYGYDAAIR
                     INEEVWHRMGKIMTREIKEKFSIEEKGLKALAKVLRYSPWTMISGFEIDEKDNEIIIS
                     IPHCASQEARLKRGLGEYSCKDMHYGEFMSIIEEIDRRIQVECLFAPPDPHPKELFCK
                     WRFTISKNESVLQDPT"
     gene            16829..17032
                     /locus_tag="PK125_03890"
     CDS             16829..17032
                     /locus_tag="PK125_03890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB37283.1"
                     /translation="MKKATAIPNRVDIDEAAIYLVHVPKCNQVNHKGREDSDCTDTKK
                     ILPDVKHEAYGFMLVHNKEQYTF"
     gene            17169..17291
                     /locus_tag="PK125_03895"
     CDS             17169..17291
                     /locus_tag="PK125_03895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB37284.1"
                     /translation="MPQTQKNPSILSGTMNVAAIIVSSHEICCLKEDEACVRAF"
     gene            complement(18355..18427)
                     /locus_tag="PK125_03900"
     tRNA            complement(18355..18427)
                     /locus_tag="PK125_03900"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(18392..18394),aa:Phe,seq:gaa)
     gene            18718..19947
                     /locus_tag="PK125_03905"
     CDS             18718..19947
                     /locus_tag="PK125_03905"
                     /inference="COORDINATES: protein motif:HMM:NF018004.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1015 domain-containing protein"
                     /protein_id="HPB37285.1"
                     /translation="MTKPFRGLLYNRNKIDDIASCVCPPYDVVSSVRTYYERNPFNAI
                     RLELPLPQPSMDKYNVAKHTLDQWLRIGVVQKDSSDTIYVYEQEIIVNEIHYLRRGFI
                     ALNKLQKERILTHEETRKKAKEDRERLITTLKTYTSLVFALYEDKEEAIEDILISSQK
                     EKLYNFVDEQSITNRFYRMTNPEEIAMLASLMESKKIYIADGHHRLAVSYRIGLDYIP
                     IYLTNMYSTGIVILPYHRIIKFNKPRSLTEVLSLLEESVLIEKFPLTDMNSPRNATQK
                     INTAAKPSYILYSKDDPDNFYILSEKAPIYTDTTVHESVRRLKVSILHMGILKNVLKV
                     QDEEISFTQALDKTVAQVKEGIHDLAFFLPPTLVEEVKEIADNSLYMPPKSTFFYPKI
                     LTGLVFYQYDGNVSSSP"
     gene            20074..20814
                     /locus_tag="PK125_03910"
     CDS             20074..20814
                     /locus_tag="PK125_03910"
                     /inference="COORDINATES: protein motif:HMM:NF025217.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HPB37286.1"
                     /translation="MTEIGFDRDETLDVLCKDKLRIIQKKNGYRFSIDPILLANFLIL
                     KKHERLLDIGSGCGIIPVYLCKKSYGNEMLGIEIQKDLYNVSLKNKTINDCRNVSFMH
                     GDIKVLAQLLKQSPFHVVVSNPPYTKEKGGRKSPQHSRLVARYETFLTLPDLMMTTSS
                     LLFRKGRFYTIYPSRRLGELIGFARLNSLELKRLRPIYPRRDKEANLFLAEFIKEGGI
                     GAKIEQPLYIFDNSGYTDEVKKYYILNG"
     gene            20817..21785
                     /locus_tag="PK125_03915"
     CDS             20817..21785
                     /locus_tag="PK125_03915"
                     /inference="COORDINATES: protein motif:HMM:NF012568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HPB37287.1"
                     /translation="METVTQLIADDLARLESTIQKLITTKIGFIKEIVNHIIRSGGKR
                     VRPILIILTARLCGYKGEKHIPYAAIIEFIHTATLLHDDVVDNAQTRRGSSTANTLWG
                     NEPSVLVGDFLFSKSFELMAHNGNEEIMRIMSEATTSLAEGEILELLKTCDIDTTEEE
                     YFEIIKNKTASLFSAACEVGAILGRVNQTKRAALRDFGYNLGMAFQLTDDVLDYTSYD
                     AVLGKRVGTDLKEGKVTLPLIHALVSSAEKEKDYINTVINNTKVTARDFVRVGKIIKK
                     YGGVEYTTNATRQFIEKAKLNLQAFKPSAYKESLATLADYILGRET"
     gene            complement(21883..23145)
                     /gene="glgC"
                     /locus_tag="PK125_03920"
     CDS             complement(21883..23145)
                     /gene="glgC"
                     /locus_tag="PK125_03920"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: protein motif:HMM:NF001947.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HPB37288.1"
                     /translation="MLLLKYTIMKSKIIQNAIAFILAGGVGARLHPLTRDRAKPAVPF
                     GGKYRVVDFTLSNCINSGVRRIFILPQYKSFSLQRHTIDAWSIFSAELGEFMSHLSPQ
                     MRVGEDWYKGTADAVYQNLYHLEEWDAEYILILSGDHIYKMDYGHFIRYHQKKKADLT
                     ISAIEVDLREAHRFGVIEVDETGRAVGFEEKPEKPKAIPGKRGKAYVSMGVYIFNRNA
                     LIDTLNKDAAMETSHHDFGRDIIPFMYPDHRVFVYRFGMVGGKDADYWRDIGTIDAYY
                     QANMDLASVNPIFNLYDKSWPIRTYEGQYPPAKTVFADEDKGRAGKALDSIICSGVII
                     SGGRVERSILSPGVRVNSYAQVDKSILFHNVVVGMRAKVKRAIIDKDVRVPDGMKIGY
                     DLEKDRKQFFVSDRGIVVIPKGAILTKS"
     gene            23225..25243
                     /locus_tag="PK125_03925"
     CDS             23225..25243
                     /locus_tag="PK125_03925"
                     /inference="COORDINATES: protein motif:HMM:NF028237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPB37289.1"
                     /translation="MNIFCVPPLLSALVLFTLFLMGIMKAKASKANILFAFICLIGCF
                     LNTDKAILTGSLHPLTALRLSRIDHAFLVFVIPLYLHFAVIVTGYRRWLPGVKICYGI
                     AILFIPVTQHNLYLIGVKQFFFGYFALAGPFFYIFGIFSSASIILSICLLVRNLKEEK
                     VAIKKTRIKYILVSFGFAAAVTHFDIVTMMGYEWYPLGNFAFIPMSLLGYAIFRHDIM
                     EWTIFLNKGMVFFTLFFMSIGFFLGTTVIVRHVLGQNFDRDLISLISMIITFFLLYVS
                     SQKVYSTITQFLQQEFILNRTALRGLSSEILTLLKISDIKTKITVELSRIFGLEKCEI
                     KAVSRIDAGENAILLDEQDSYWIQGFRLSIAIPSKSNPSYLLLGEKHTVNLYTTEETE
                     VLIMLANNVALAFDNADAYTKLQEFSASLEQLVNERTRALIQSESLAAVGRLAAGVAH
                     ELNNPLASVMSTIEYQIDHLTDNEQMREDLEFSLQELRRAADIVKSLLDASRQKEETK
                     ELIDLHIPIEDALKVLYNQYKNKKITVIRKFDAERSFVRGNTSRLCQVFINLIKNAID
                     AIGEKEGHITIETANKELPLGNNYRGVNSGDVIVCKIIDDGEGINEKNIHNIFKPFFT
                     TKSQGKGIGLGLFIVHEIVKDHKGSITADSNPGRGTVFTLIFPSHQQS"
     gene            complement(25215..25892)
                     /gene="rsmI"
                     /locus_tag="PK125_03930"
     CDS             complement(25215..25892)
                     /gene="rsmI"
                     /locus_tag="PK125_03930"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: protein motif:HMM:NF012799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HPB37290.1"
                     /translation="MDGVLYVVGTPIGNLKDITLRAIEVMKDADLIVAENRERALKLL
                     NHLSIHKPIITINSYNEERRAKSIARQIGSGKSGVLISGAGTPCISDPGGFVVRACYE
                     EGVEVKTVPGPSAATGAISICGLYVDRFFFYGFLPQRPGKKRKALRDLSSFPYPIIFF
                     ESPRKLLETLRCVRVEFGNRPVVVVKEMTKIYEQVLRGSVDDLIATLNDIELKGEYLM
                     IVDGMGK"
BASE COUNT         7667 a         5893 c         5281 g         7295 t
ORIGIN      
        1 aatatattag aaggctaaaa cgtgatggcc atgatgtacg gaggtatctc gtcgacttgt
       61 ataacaaaac agcattcccc tttataaacc tgataatggt gtttgtggcc ttttccgtag
      121 gacttcgata ttccaaaacc aaacatattg ccaaaggcat tctcattggt gtatgcgttg
      181 gcatccttta ctggttcatt cataatatct gcctgtcctt tggatatgct gagacatttc
      241 caccgttatt tgcagcatgg ctatccaata tgctattttt tttcgtgggc attattgggg
      301 tcgtcacatt gagaacatga gggaaactga gtgtggactg ctattaatta ataactgccg
      361 ccggcagcgc aaaacgaaag aatgggcaag ggtcagctca tgaaatacag ggcagaaagg
      421 cgagaacaaa acatatgaag gcatatcttg acattgaaac agacagggaa ggcaatatca
      481 gtgtaattgg catttcgatc gggtctaatg gattcaaaca gtttgtcggg aaagcgatca
      541 ccagcaataa agtagaaaac tgcttgcgtc aggcaaagac catagtcaca ttcaatggtg
      601 acagttttga tcttccccaa atcaaaaaac aactcaattt ggatcttgca gtatctcatc
      661 ggtcgtacga tctcttcaag gtgaagaaat ggctcaacat taaagggggt ctgaaagaac
      721 ttgagaagat gtacggtata gaaagaaaaa ctgaagggat agacggattc aaagcagtcc
      781 agttatggga aatataccga agacatggca gaaaagatgc tcttgaactg ctccttgaat
      841 ataacaaaga ggatgttctg aaccttatcc cccttgaaga gaagtttaac acactgataa
      901 aggagcaatt ccatggtcaa aaagtttgaa gtctcatcag cgcataacaa aatagcaggg
      961 cttatgatca ttccgccggg cagaaaaaaa cacccctgcg taattctctc ccacggactt
     1021 gtcagctcca aagagagctc aaaatatgcc cttgtatcag aagtattcgc tgaggctggt
     1081 atagcaacgt gccgatttga ttatcacgga tgtggagaaa gcggaggcga tattactgag
     1141 acaaccctga cgattcgtct tgataacctc gatgtgataa cagagtatat tataaatcac
     1201 ccttctattg accctgcgaa cataggcata ctcggaagca gctttggagg aaccgcatgt
     1261 gtattgaagg cagcaaaaga taagcggatc aaatgcattt ccccgtgggc aaccccttac
     1321 aggctggata aggatggaga cggaaccata tcaggtattc cgttcaaaga gactattttt
     1381 acagattttc agaagtatga cattcttttt gaagcgagaa aggtatcaga tgcgctcgtc
     1441 attcacggtg aaatggatga agttgtgccc tgcgaggaag ggaaaacaat ctataaaaat
     1501 atgaagaaac caaaaaaatg tgtgataatt aagaacggcg atcatgtgct ttcagaccct
     1561 attcaccggg acagagcgat caagctcggc cttaactggt tccagggata tttcctgaaa
     1621 gggtagattc ttcacggcaa aaaactcagt aaaaaattaa ggattaagat atcagcgggg
     1681 ttgtgggtta attagctcgc tcagcgagcg cgaagagaag gctttgacag ttttggaggt
     1741 ggagaggaca aacactcttc caaagctcct gcgtgggttg ccacaggttg caggaacagg
     1801 taagctactc cttttttttg tctgttttta ttccaagcct gtccttaata aggtcaccta
     1861 caacattact caaacctatg ccaagggcaa gtgagagggt catgataatg ccaccgaaaa
     1921 ttatgagaaa acttatggtg ataattgtat tgcccactcc tatatattca aaaacaattc
     1981 cgaatgttat gataacgagt aaaattttca cgccccttcc gataggctca ccatatttga
     2041 tatttgcatt ttcacaggcc aggtaaatga ccctgctaat aaaattgctc aaaataatgc
     2101 caatgataat gataacaatg gaaacaacaa tgagcggaag tgcgcttgat acgcgtgaag
     2161 catattctgt gaactggctt attccaatga aattcaagcc aaatgagaaa aagacaacaa
     2221 taaaaatcca gtaaacgatc ttgcttatca caatagatgg cagggtatga actccaccct
     2281 tttgtaaaaa gttgataatg ccaacctcgt ttgcccattt gtcgaatcgg aagagaacaa
     2341 gaagccttgc taaaagcttt tttatgaacc agcttatgat aaacccactg agcatcacga
     2401 tgatcatcac aatgatattc aggaaaacct ctgtaaagtt gtcgtacaga tccagaaaca
     2461 tatccttgaa gaaatcgatc atgagaacaa tcttattcga tgtatatagt gaagtcaatg
     2521 aaaaacacta ttcaattcat agcctccata acataaaata agcaatggga taaaaatcca
     2581 gcttgccgaa atatgtttca atatttccct ggtcaccaag aaagaaatag gattttaaat
     2641 ttttttattg acattttttt cagattcgtt taggaattct aaaatactca aaggaggtaa
     2701 acgatgcctg caaaagttga tgaagatgcc tgcacgggat gtggggcgtg tgctgaggtg
     2761 tgcccggtcg atgccattac agttgatgac acggcaaagg ttgatccgga aacctgcact
     2821 gaatgcggga cctgtgtgga tgaatgtccc gtagaagcaa taagcatgga agaataaggt
     2881 tcacttaaaa tataaaagga tgggcaagcc catcctttta tatgtcttac accaatatac
     2941 tctctattta atcaatactc tttacaacaa gctctgagat atctaatgct ttcatggtct
     3001 cagttttttc cctgtccttg atagcatctt ccatcatggt gaggcagtac ggacaagctg
     3061 tcacaacggt gttggcgtta atggcaatcg cttcatcaat cctcaggtgg ttcatcctca
     3121 gatgatgctc ctccatccac attctcccgc caccgccgcc acaacagaaa ccttcatcgt
     3181 gatgacgacc catctcaaca tagcttccct tcttgaactg attcaccaca ctcctgggtg
     3241 cgtcaaagac ctggttcact ctaccaagat agcatgggtc gtggtatgtg gtaataccct
     3301 cgagattggc gttgatcttg attttgccct cctgtgcaag cttagctata agctctgtgt
     3361 gatgaaagac ctcaaaattt gcaccgagtt gggcataatc gtttttcaac gtattgtagc
     3421 agtgtgggca ggctgtaact atctttttga ctcccagttc cttcagcaat tccacatttc
     3481 cttctgccag ctcgaagtac tggtattcat tgcctgttct ccgtaggggg tcaccgcagc
     3541 atttctcctc cgggcccagc acaccaaatg agacgcctgc tttctggagc accgttgcaa
     3601 ccgacctggc aatttttctg ttcctatcat ccaaagatgc cacgcagcca acccagtaca
     3661 agtattcgac ctcctcttca gtagcctttt tgatatcaag gccttcggtc cactcaagtc
     3721 tctgggattt acccattccg tatggatcaa accgggtctg gagatttttg taaagaagct
     3781 tcagttccga gtttacttta ctcaatgtca taatgaggta ccgtctcatt tctatattct
     3841 tgtcaaaagt agggattgat acaggacagt taacctggca ggacaggcag cccgtacatg
     3901 accaaaccgt ctcttcctgg attacattgt caaggaggcc atcttcgttt ttgatgcctg
     3961 ctgctgtctt ctcccactcc cccttcaggt cctggatgaa tttcttgggt gaaagcggct
     4021 tttgcgtcag atgtgccggg caacgatcct ggcatcgtcc gcatcgtgta cacgcatcaa
     4081 ggtcaaatat ctgcctccag gtaaaacctt ctatattatt tacgccaaac tcctctgcat
     4141 tctcaaaatc aggaattggt gcaatagcgc ctctcggttt gtcttcaacc cctcggaaca
     4201 tcatgttgag ggaagaggtg agaatatgca tcaatcttgt tgaatacgcg atatacacaa
     4261 tgaggccaaa gcttatgacc atatgaaaat accagaggat caggtgggtg gtctcaatag
     4321 atgctttatc catgccgctg aaaaggcctg ccaagaacca gcccacaaag ctccacacct
     4381 caaaatcggg ttttgttgca gcgatccttg cagcctcaac caaaaaacca gatacaagaa
     4441 ccaccagtat ccatatgaga cagataaaat catcaggctt attgtcattt atcctttccg
     4501 gctttgttac atagcgtctg tacatggcca tcagaacgcc aatgatagcg gctaaacctg
     4561 cgagctcgag aaaaaatgag aaccacagat aaaaatcgcc tttgagatac tcccagccaa
     4621 atacgaggtg ggtaaaatgg tgctggaatg catcaaggaa tgcaccgatt gcaagaatca
     4681 ggaagcccca gaaaatgaac aagtgcatcc agccaggaaa gccctcacct tttctcagga
     4741 tggtcttatg aaaaactcca cttttaatga agtatccaat cctctgacca agcaacttca
     4801 taaaatcaaa gggttcgctt ttcccgatct tccacatatt gtatcgcttc ttaagaccga
     4861 gaaaaagaat aataaaggaa attaccataa gcgagtaagc aatccactca cccgttgatt
     4921 tgacgttcca gaagatctcc ctgcttatct ccatcatcct cctcctagac ttttttgcga
     4981 tatactacca caaaatattt tcgttgtgaa aacaaattca tacttaattg tgaaattatt
     5041 tacaagattt catataacct tcgccttcag tggttttgac gtggacatta agttatcaat
     5101 gcaaaaatgc gaaaaaatat cttgacatga tgtcgcacct tctgttatga aacactaaaa
     5161 taaatttcgt ataggtaacg gtgatagaac aggagtaatg aaaaggactg tcttcacgtt
     5221 tgtaatatgt tgttttttta cctttatctc ggtaaatctt gcattatctt ccaatgttac
     5281 atacaaggta aaatctggag atacgctcca gaagatttca aaaaaatttc atgttcctgt
     5341 tgagaagtta aaaaatctca acggcctttc ttcaacaaaa ctgaaaaaag gtcaaaccct
     5401 tgtcattaaa aaagatgttc cagcgagaaa ggaaagggaa acggcttctg caccaccggc
     5461 aggcaaagat tctcccgttg atctaactac agcagaaaat gatggggaat ttattgaata
     5521 tcggccaaag aaaggtgata cccttgcaaa gattgcatcg aagtttaacg tggataagga
     5581 agatttactc gaatcaaaca atataactgg cagaaggctt ccgaaggtca tactggtccc
     5641 caagatcatt gcagttgatg attctgagga tatcgttgat ctctcctgcg gtaagcctct
     5701 aaaaccatgg cggaacaatg atgagaaata catgctcgtg aaggtagcca aggggtttat
     5761 gggcgctcct tataagtatg gcggcgaaac cgtccgtggt cttgactgtt ctgcatttgt
     5821 taaaaaaatt tacgatatct ttgatgtaca acttccaaga agtgccaggg aacaattcag
     5881 cgtaggacat aaaatagcaa aggaccagct aactgtgggt gacctagtct tctttaaaac
     5941 aaagcgttac atcaaatatc ccacccatgt aggcatatat attggtgatg gaaacttcat
     6001 ccacgcatct tcaaatcgca acagacttgg cgtaaaagtt gacgcccttt cctccgactt
     6061 cttttcaaga acatttatgg gggctacacg ggtgaaaaaa tcacctgatg acacagcaga
     6121 aacctccaaa aattcacaga atcagccctc aaataattcc tagtatttga acaaaaagac
     6181 cacaccgatt ctccttttct caatcctaat ccatctctcc ggttactatg tactcatcga
     6241 aaggattgag cctttccagt attttgctta tctcatcctg tggtggtcct atattatctt
     6301 catcgatgca gtccttgccc ggaaaatcaa gcgtttccta attttgaaca aaaacctgcc
     6361 tttcctgata attatttctt cgggttactg gtgtttatat gagattatca acctcagact
     6421 tgagaactgg ttttacataa accttcctca caacttcttt cagaggtggg caggttatat
     6481 gctcgcctat ggcactgtca taccagccct ttatgtaacc aaagaactgc ttcttgaaat
     6541 actgggcgag atacaggtca aacctcgacc catgagggct taccagaaat tagctgttta
     6601 tgcaggtatt tttgcactca tcatgaccat agtgtttcct atttattttt ttcctctcgc
     6661 atgggtcttc cttattctga ttatggaagg ttgtaattat caaaaaggtt attcctcttt
     6721 catgagagaa attgaacagg gtttattggg aaacctcatt gccactatcc tgtcagggtt
     6781 agtctgtggg attttatggg aaatctggaa ctattggtcc atttccaagt gggtatatac
     6841 tgttcctttc tttgaagacc ttaaaatatt tgagatgcct ctgcctggtt actttgggtt
     6901 tctattcttt gctgtgggga ccattgcctt catgaatttt cttcagggaa tcaaagtgta
     6961 tagcttatat ctccttcggg taacatctgt agccctagca ctttccttat tctcatttat
     7021 attgattgaa agaaacacag tcttttcata cacaacgagg ctcgatcagc tatcttttat
     7081 agcaaaggaa aaacttgata cattgaagcg cgcaggagtt caaataagtt ttggaataaa
     7141 ccctcagcag ttagatccca tggaaaagga cgccctcgac ttgattcatc tgaaaggtct
     7201 aggatatgac cacttcctca aactgcgaga ccgtggaatt gacaccatac agaaactctc
     7261 cctgacggac gaaaacgcaa tctcatccat cctcgatgaa gcgaatatga gaaggatacg
     7321 ggtctacttg aaggcggcta agaaagctcg ttactgattg atccataaac cgtgttgcac
     7381 aatgcgtgat cgattatcag ctctttttca cgtatacata ccgccggtta catagtacag
     7441 ttattatttc ttaactcttg cttgccctct cttttccata tctgctccta tttcttcatg
     7501 ccttccgtct cgcttcttgc cttattactt cacttccttt cccgcacgtt ctgctttcct
     7561 gtccacttaa acattgacag tctttggtta attatggtga aataccgact atggctaaga
     7621 aacagacgaa agacttgatt agaaaaccag actttcttct ccagtttatt gaaaacgcat
     7681 atatttttat ccaggagaat ttacgcggtt ttatcattgg cgctgttata tttgttttgg
     7741 cagttgcttc ggtttatggt tatgcggtct acgcgcgtaa acaggaggag aaatcccaga
     7801 ccactctgtt tcagggcata aaaagctttg aagaatacag ccagggcggc aagcaagaaa
     7861 gtctcacaaa cgctgaaaat gtattccaaa cccttattaa agaaaaaaaa ggtaaggcat
     7921 ataagattgc caggctttac ctggcaacca tttacactgt acagggaaaa tcagatgacg
     7981 ctaagatgct ttatcagcaa gtgataaagg attcttctgg cacaattctg caaacactgg
     8041 ctgaacaagc actccagggt ttagagaaaa agtagccatt agccaacacg tgcaattgat
     8101 tatcaggctt catccaaaaa acattcgaac cattcccatc cccaataaaa caaccatctt
     8161 aacccttatc tgtatggcaa aaacaaaagg gtatgacaga atcgcgctac cgactaatgg
     8221 ttggaagctc tggctgttag ccgtataaaa cagccaccgg ttcatatttc gttccctgca
     8281 ccgcagggta gatgcctgcc agaataccaa cgagtgtact tattaagagt gagactacga
     8341 tattttcaag gtaaagcgaa aacggtacat tcccaaaata gcttacgaag acagtgatta
     8401 taaaacccac caggattcca agggcacctc ccataagagc tacaacaaca gactcggtaa
     8461 gaaactgcag aatgatgtcc cttttcttcg agcccacaac ccgtcgtatg cctatttcaa
     8521 gctttctctc ggaaatagaa agaagcatga tggcaaaaat tccgagtcct cctattaaaa
     8581 aagagacgat ggaagcaatc actgtcagga ttgataccag acggatacct tgttcctgtg
     8641 ttttaacaat gtcttccatg gtaaaaactg aaaaatcatc tttctgctcg ggcttcatat
     8701 tacgaatcgt ccttataaag ctctgcagga tctgcttcat atcagtcagc gggatcttat
     8761 cctgtatctg aatatatgcc cctttgatga aatccacatt gctgtaccgc ctcataatag
     8821 aagtcaaggg cacatagacc tgtaggtcct gatcctggcc tgccagatca gtcccttttt
     8881 cttttagtac gccaataacc tctgttggga ctcggtaaac aagaatgtat ttgcctatag
     8941 ggttctcctg ttgaaagaga ttctcaaata ccttgtaccc gacaacagct ttcttcctcc
     9001 cattcacgtt atcgtcctct gtaaaatacg tacccataag caaatccagg ttccttatct
     9061 gaaacattgc ttcggttgta ccgacaacac ctgcagtgac tgtcttttct tcgtagcgtg
     9121 caggatagga tacgtcgtag aaaggtgcaa tctgaacaat tccgggaacc gactccccga
     9181 ttctctggac agtctcaagt ttcaaattct ttgaatcacc gaagggcata gcgcccctcc
     9241 ctgtgaaacg catgtaaccc gacctgagaa taataagatt ctttccgaat ttgcctattt
     9301 cactatcgat ctgtttctta agaccattgc ttatattgcc aaaagcaacg agactcatta
     9361 ctgcgaagag tattccaaga agagcaagaa tcatccgacc cttatgaacg atgaggttct
     9421 ggaaggctat cctgagatag aaggcaagcg tgtatttcat ccccgtattg cctcaatagg
     9481 ctctatcctg ctcgccttca atgctggccg aaaacccgca attaatccag taagaaaact
     9541 gaacataagg gaaaaaacaa caactttgta tgaaaacaac atgggtatat caaagagtgt
     9601 ctcgaatgtt ccacccagcg ctaggctgag caaaataccg gctatccccc ctaaaagcgt
     9661 tatgatcacc gactcaagga gaaatgagag gaggataccc cttcttgacg ctccgtaggc
     9721 tctcctaatg ccgatatcct tccttctctc ctgtatagta agataaaaaa gattggcaag
     9781 cacaaaacca cttaccacaa gcgcgactat agacgccatt ccaagaaaaa gcagaagctg
     9841 gcctgatatg actgttacaa acttcattat gtcctgtgaa ctccttatag taaaatcatc
     9901 ttcagccgac ccctgaaggc cgtggtttct ccgaagcacg gccttcacgt ccgcaacagt
     9961 ttcgttcaca tccctatttg tctttaacct caccccgctc agatacttct tatcattaac
    10021 aaccctgctc ataaccgtgg taagcggcat aatcaccctg tcatctattt gtgcgccccc
    10081 ggttccaccg cccctttctt tcagaacccc taccactgtc atgggaagcc tgcctgcaag
    10141 gatcaccttg ccaatagcgt cttcaccctg aaaaagctca tcatatacct tcgatcctat
    10201 aacagtcaca gcccttgcat atttgtaatc atctgtactg aaaggagagc ctgcctgaag
    10261 gtcccacgac atcgattcaa agtaatttgt cgtggaaccg attatacgag actgccattt
    10321 tcggtcttta tacttcattg ttggacgcct cgcataataa gtcttcacag aatcgtagac
    10381 tccttcgatg ctatcaaggc tttctgcatc tgcaagtgta agtgtgctcc ctctcattcg
    10441 cgctgttcgt tgcctctctc cacctcccca aaccataata gaatccggac caagtacttc
    10501 aaatatatcc ttggcttttt tatttgctcc atcaattgtg gtaatgatga cacagatgga
    10561 aagaatgcca agagctaccc cggcaaatga aaatatgacc cgtccccgat agtaatagag
    10621 gattttgaga atctcctgaa gaaattctct acctcgtgat atcttctgaa atagtcccat
    10681 cttctatcct gatcagtctt ttgccaaagg atgcgagctt ggggtcatgg gttaccacaa
    10741 tgactgtctt cccttcgtca tttagtattc ggaaaagatc cataatctca cctccggtct
    10801 tggaatccag agcaccggtt ggttcatctg cgaggatcag gtcaggttcg ttaatcaagg
    10861 cacgggctat agctacccgt tgctgttcac cgccagagag ctgggacggt ttgttgtaaa
    10921 ccctctctgc gaggccaaat ctgctgagaa gtgcttttga cttttcacgg ggatgtttaa
    10981 tatccttgga agaataaacg atgggaataa ggacattctc aatggcattc aaatagggga
    11041 gcagaaaaaa ctgctgaaaa acaaagccta tatgcaggtt tctcatggat gcaagtctct
    11101 catcatccag gccgttgatg aaaacatcca tgaatctgta ttcaccttca gtctgcttat
    11161 cgagaagtcc aacaatattc attaatgtgg tcttgcccga accggaaacg cccataagaa
    11221 caacaaactc cccacttcta atttcgagat cgattccctt gagaatgggc agctcgcgtt
    11281 ctcctataaa ataacttttc tttatgttcc gaagggtaat cacttcttgg agcctggcga
    11341 agcattctgg gaaggttttc tgacgacaac cggtaccact atggtttcac cttcctcaag
    11401 acctttaacg acttctgtaa acctgtcatc ccggatacct agagttacct gcctagcttc
    11461 tgttttatcc ctccctttga gatagactat cgtcttacct tcttcaaatc ggacagcatt
    11521 attcgggata accagcacat cagttttttc ctctacaata attcgcacat gggttgtcat
    11581 ttctggtctt aaaaaagctg tatcttcagg ggctatcttc acaattgcga ggtaataaac
    11641 tatgttatct ttaatttctg gttggggata aatcatgtcg atcttcccat aaaacctctt
    11701 gtcacgaaag gtatcaaccc agtattctat ttttctaccg ggtgtcgtcc gaccgatatc
    11761 agtttcatcc acataaatcc acatttcaag cttggaaggg tctataatgg tgatgagaat
    11821 cgcagcggaa agacctgaaa ccacggtctc accgagctgc gttgaaactg ccgatacata
    11881 cccggagatt ggagcataga tctttgtgta acttagggat acttcaaggg ctttgcgtcg
    11941 ttcagcagat tcttttattt ttgctgtacc atattccacc tgtgcaaggg ccacatccat
    12001 ctcacgcttt gctttgtcaa cgctatccct ggtagtgaat tctttctcca aaaggctgag
    12061 ttctcgttca tagtttgtct tgctgtagag gtattgtgcc tcctttgctt cgagatcctt
    12121 cttcaatgac tctatggtag cttctgtatt tcttatgttt gccagaatct ctcggtcatc
    12181 aatcgtggca atcaattcac ccttttttac cgcatcaccc acctgatact taagataagt
    12241 gagtgttccg gttgcccgtg tgccgacctt gacaatagct ccaacttgag ctttgatgat
    12301 gcctgtctgt tcagtgaaat gggtaacatt gccccgcttt acagtaacat cctggccagc
    12361 ttttccctgg ctggccttat tgcctgaaaa taaaaagacc agtacacctc ctgcaatcag
    12421 gattatcgct gcgataacaa catatttctt cttcatgatc attctctgcc tatataggca
    12481 acacgctcca ggcttgccag gtttgtattg gccctggaaa tggcgcttac caagttttct
    12541 tttgctttgg caaggtccct ctcactctga agaagggcca taatgtcacc ttttccaact
    12601 ttatattccc cgaaggcttg gtcaaagttc gacgtagcct ccctcaccag ttctccataa
    12661 agggttacat tttgagcact cagttcatag tctttcaatg cctttataat ctccacgcta
    12721 acagttcttt ttatctcttc gagcctgtat ttcgcagcgg caatatcacg tgatgccccg
    12781 agcatattgt agtaacggcc tataccgtca aaaagaggcc aggagaagtt tatacggaac
    12841 gtttcctgcc ggccttctgg aaaaaactgg taatcctgcc gcgtctgttc gagctgggtc
    12901 ttgatcctcg ggaaccatgc gctcatcttt tcccggtaag ccatgttcag tcgctcaatt
    12961 tcgttcttct gtactgtcac gtcaggcctg atattaattg ccctctcagt caactgctga
    13021 tagtctcctt tgtattgcgg ttccatgaac ggaccatcta cctcttgcct gtcttcaggg
    13081 tcatacagaa ggaggctctt gaattcagca agtgtttttt catactccct ctgagcgtcc
    13141 atgtactcga tcctggctgt tgtcatgcga acttccgact ggaggacatc gctcttcttt
    13201 gctacacctg cactgtatct ccctttcgta agggctaaca ccctctctgc cacattaaat
    13261 gcctcttgtc ttttctcaac aatcgactta tttcccatta cagtatagaa ggctgcctta
    13321 acaaggtaga gaacatcgtt cttcacaccc tggagacgtt ctttttctcg cagagtagaa
    13381 aaaaaagcgc cttttctgcg ggcatagcgc tcacctccat caaaaagtag ataagagatt
    13441 gatccattga gtgtgtatgc atctctcgac ccggaagcac ctgaagtgct gcttccgctc
    13501 agaaagctta ccgaccgctt gccattgaga gacctcacat acgaactata aagttcaacc
    13561 tgtggcaggt aggggtccat agtagagata tatgaatact ctgttttttt cacaatttct
    13621 ttttgttctt taatgagaaa agagatttca agcgcccttt tgatggcttc atcaagggta
    13681 atggcacatg cttcacggct caaaaagcta acgatcatta tacagacaaa aatgattcta
    13741 cgtctggata aaaacattct ccctccaaaa aaacatcaac agtctatcgt tatttccttt
    13801 ataaatcaaa tcattctgca tatatcgaca gcaatggatc ttcataattc agtctggtat
    13861 tgatgtcttg tatctggttt tttgcaaaag aaatgccaat aacctaaaaa cagattcttg
    13921 tttgcactgc cagaaatcag tacctttggt caacgataag cctgtatgct tatcgatgcc
    13981 ggagttaatt gttttttcct gtttgattgg caggatgatt ttcttaagac atggttactt
    14041 agatcgacgt ttttgtcgta attttttttg aaaacgacaa aattgttaat gggtaacgac
    14101 tcttcgttcc tattagccat taatttcaat atattatgtt caagcattcc gggcatgtgt
    14161 attgctatgg ggacattaca tatccatagt atttgggata caaacagcca ggaggatcaa
    14221 gatgaaaaga ttgctttttt tgataatttt tttttcaatg atgtgcattg cgccaagcat
    14281 aggtcatgct caaaaacctc actatggcta cccccacgga tattggaggg gttcttatta
    14341 caggccacat catagacctt actattatcc ctggtatgga tcgtactatc ggctgttgcc
    14401 cagacattat tatgccccgg cacctgcata tgtctatcct cctcaccctg taatatacgc
    14461 accacctcca gcagttataa tcgttccaaa tcaggcctat gcttaccctg atatcccacc
    14521 cccaccccct gccccatcct ctcaggttac ctcgccccta caatcagttc caacagctat
    14581 tcttcagtca atctattttg atctcaacaa atctgctatc aggcctgacg ctgaaaaaat
    14641 cttgaaacag aacctcgaat ggttcagaca gaaccccggc agaaaggtga gcattcaggg
    14701 aaattgtgat cctcgggcaa cagaactgta caacctctcc ctcggcaaaa gaagggctga
    14761 agcagcacgg caataccttg ttgaacttgg tgttgacact aaacttctcg atgttgtcag
    14821 tttcggaaag gaaatgtcaa cttgcaaagg aaaggatgaa tcctgctggg ctaaggaaag
    14881 gcgggttgat tttatacctt caccctgata acacccggta tagagaagaa atggcccctt
    14941 atcgcataag gggcgcgtta aaggaaaaat gactttcacc tcccttcata ttttgcaccg
    15001 ttctcaagta ttctgttata ttaacccgtg ctgtgcttcc catggaattc tacgaggtag
    15061 gtattcatga agagtctttt gagaacaaaa gtctttctga ttgttgctct tgttttactc
    15121 tgcctgcttt tgatactgac gctctatgcc gatgagaccg ccgtggttac agatattgtt
    15181 gacggcgata ccctggctat tgagtataac aacaggaagg aattgataaa tctgatcggc
    15241 attgatgccc cggagagcgt aatgaacagg aaggcagaga ttgactccag aaccacagga
    15301 gaaagcctgc tcaccatcac gtccaaaggc atagatgcaa aacgttttgt tgaaaggatc
    15361 gtcaataaag gagacacggt gttcatagct ttcgatgccc aggcaaaaga ccaggaggga
    15421 aaacttcagg gctacgtcta tctttccgat ggaagacttc tcaatgaaga agttgtcaga
    15481 gcagggtatg ccaaggttac agcttccttt tccaatccaa aatttaggga tagattcatg
    15541 gaggcttata gagaggcaag aacctttagg cgcgggctat ggaggtccca ataggtacca
    15601 agaaaaagaa caaaaaaatc gattgataag tcaacatcct gttttaacat ctcgattttc
    15661 aatgtacggg agcctctgtg gaacgcaatg ggtccttttt tttgtttcaa tcattgtttc
    15721 tccatgtttg ctgtttaacc tacatttgtg cttgacatgt ctgctatttg tgctattaaa
    15781 aattatacac tctaagatac ttaataaaac aaaaggaggc tattatgagt gtctatgttg
    15841 tgaatgaaat tgtggggacc agcacaaagt catgggaaga tgcaacaaaa caggcaatag
    15901 aaactgctgc aaagaccgtc tcagatctcc gtattggtga agtggtgaaa caggacgtga
    15961 cagtagaaaa tgggaaagtg tcgagctatc gggtacgact caatatttcg ttcaagtaca
    16021 aagaaaaata gtctggttga agaaaccacc tgtttcatgc agggccgcag tggccctgca
    16081 ttttttatcc acatttcaac gagcactacc cggtaggaaa aagttgtctg cacctcatgt
    16141 agggtcctgc aacacagatt catttttcga tatagtgaat cgccatttac agaaaagctc
    16201 tttcggatga ggatcagggg gtgcaaacag acactcgacc tgaatacgcc ggtcaatctc
    16261 ctcgatgatg ctcataaatt ctccataatg catgtcttta caactgtatt ctccaagacc
    16321 cctctttagc cgagcctcct gagaagcgca atggggaatt gaaataataa tttcattatc
    16381 cttttcatca atttcaaaac ctgatatcat ggtccaggga gaatacctga gaaccttagc
    16441 aagggctttc aacccctttt cttcgatcga gaacttctct tttatctccc tggtcataat
    16501 cttacccatt ctgtgccaca cttcctcatt gatcctgatt gcagcgtcat agccataagc
    16561 ctgctctaca cctataaacc aaaacccgtc agttcttctg aactggttca ccataaactc
    16621 gatatagggt aaaagctctt cgtttctgaa ttgaggcagt ttcatgtgcg tttttcctcc
    16681 tttatgctgt tgaccatcct tgctcgggaa aaatgtagct ttctcttaca gattctattc
    16741 cccaatgaag tctgtgtcaa ttatctctta taaacgggag gctaacagga agggtgggaa
    16801 aaatcaaaag tggattaaga agcttacaat gaagaaggcg accgccatac cgaatagggt
    16861 tgacatcgat gaggctgcca tctacctggt acacgtgccc aaatgcaatc aggtaaatca
    16921 taagggacgg gaagattccg attgtacgga tacaaaaaaa atattaccgg acgtgaagca
    16981 tgaagcatat ggatttatgc tcgtacacaa caaagaacaa tacactttct aaagaatgtc
    17041 cttaacatgc atactttccc aaataaataa ttgtagtaag aatacatgga ggtaaaggat
    17101 attgtcagat acaactaaga taatttccat aaccacgcta caaaataagc taacacagaa
    17161 gatgatggat gcctcaaaca caaaagaacc catctatact ctcaggaaca atgaatgtgg
    17221 cagcaatcat cgtatcatct cacgagatat gctgcctgaa agaagatgaa gcctgtgtca
    17281 gggcgttttg aaatattcga gatggttcac aaacgtatga agcgctatga tgcgtctcga
    17341 aatgtatccc ttgataagat acagaaaggc aatggcctat agggttttat tcacagaaga
    17401 tagagaagat tatttcaaga aatcgatgaa tcaatcaaga aggtggtcaa gaaggatagt
    17461 ctttgcagaa aaaccctttt atgggaaata ctttggtcat gagtttggcc tgatccgata
    17521 ggacattata aaccctatgt ggcaaagcag cttaccgtat aatctatcac catattggag
    17581 acgaaacaga gtttatggag atggtgggca tagcaaacgc gaaaaatgag gaaatctact
    17641 ggcttgtgtt aaaaagattg agaactgtca aaaggaaatg aatattcctc cattgtgcct
    17701 ctcgatatct ggaatcaaaa gggggtgaag tactggagaa aggacaacag aaaggacgcc
    17761 acatcaatgg ggtgatctac aaaaaaaagg taagatttat tagataaagt actatcgtaa
    17821 caaaaaacca ttctatggaa attcaaaatc caagaagaaa cagatgcaaa gcaacttctg
    17881 aattacagga aggataaatc gctggaggct gtttcttcgg cctcaaggcg gaaaaagact
    17941 tctttaaaag actggcgaat gatctgatgc agattactga acgaatggga acaaatcatt
    18001 gatacgagca gagcgaagtg atggatatgc acgtttaaac aggttcagat tggggagaag
    18061 ttgttttacg gctttaagat ggacaacgat tatgaacatg ataatatggt gagggcgtga
    18121 gtgccagaat gtgtagcgat gaaaacacag tcacaagatg cccttagcat tgacagatgc
    18181 aacattgtga acgaggatgg cctggaatcg gcacgtgaat gtttgtgaga gatcctcaaa
    18241 aaagataaga agataaccga aagccaagat atggttacaa atgtagttat agtcccaatc
    18301 ttacgtcatg ggattagtct gattttgatc gcggtatatc tgatattaaa aaagtgccga
    18361 ggaccggaat cgaaccagtg acacggggat tttcagtccc ctgctctacc gactgagcta
    18421 cctcggcacg ttgttattta aaacacactt gttcgccgga tgtcaagaat aaagaccgtg
    18481 atcggcaaca cgcgatcagt gaaagtcgta tatcgttacc tgcatatcgc atctcgtaaa
    18541 aggccatata taatatttta cgtatagtgc gaaaatgaat ctgcacaaaa tataaacgat
    18601 gatatgcgaa atggggtgtt taacaacata ctgcatggga tatactcaac atcaagttct
    18661 tcaaaacctt ttgacttttt atcacctcta tgttaacaaa ttcacatgaa agaattgatg
    18721 accaaaccgt ttcggggact tctatataat cgcaataaaa ttgacgatat tgcgtcttgc
    18781 gtgtgtcctc cctatgatgt cgtatcgagt gtccgcacct attacgagcg aaacccgttc
    18841 aatgctatac gcttagaact tcccctgccc cagccgtcaa tggataaata caacgtcgcc
    18901 aaacatacct tagaccagtg gttgaggatt ggtgtggttc agaaggactc tagcgacaca
    18961 atttatgtgt atgaacagga aatcattgta aatgaaatac actacctgag aaggggtttt
    19021 attgctctga acaagcttca gaaggagcgt atcctcaccc acgaggagac gaggaaaaag
    19081 gcaaaagaag atcgagagcg gctgataaca acgctcaaaa cgtatactag ccttgtcttt
    19141 gctctctacg aagataaaga agaggctatt gaagacatac tcatcagttc gcagaaagag
    19201 aaactataca attttgtaga cgaacaatcg ataacaaaca gattttatcg gatgacaaac
    19261 cccgaggaga tagcaatgct tgcatccctc atggaatcaa aaaaaatcta tatcgccgac
    19321 ggtcaccaca ggctggctgt gtcatacagg attggcttgg actacatccc aatctatctc
    19381 accaatatgt actcaacagg aattgtaatc ctgccatacc ataggattat caaattcaac
    19441 aaacccagga gcctcacaga agtattatcc ctcttggaag aatctgttct gattgagaag
    19501 tttcccctga cagatatgaa ctcgcccaga aatgctacgc aaaagattaa tactgcggca
    19561 aaaccttctt acatactcta ttccaaagat gatcctgaca atttctatat tctgtcagaa
    19621 aaagccccta tctacacaga tacaacagta catgagagtg tacgaagact caaagttagt
    19681 attctccaca tgggcattct gaaaaacgtc ctcaaagtcc aggatgaaga aatctcattt
    19741 actcaggccc ttgacaaaac cgttgcccag gttaaggaag gcatccatga tctagcgttc
    19801 ttcctgccac caactcttgt tgaagaggtc aaggagattg ccgataatag cctttacatg
    19861 cctcccaaat caacgttttt ctatccaaag atactaacag gcctcgtctt ttatcagtat
    19921 gatggcaacg tatcttcatc cccataatac tgcctatttt gtccaactac ccccatttag
    19981 tatccgccga tataccgaat atgcactctt cccttatttc tccctgtggc ccggaccctt
    20041 gttagcgtac acgatttttg aattcaaatt ggcatgactg agatcggttt tgatcgggat
    20101 gaaaccctcg atgttttgtg caaagacaaa ctccggatta ttcagaaaaa aaatggatat
    20161 cgtttctcga tagatccaat ccttttggcc aattttttaa ttctcaagaa acatgaacgg
    20221 cttctggata ttggatcagg gtgtggcatt atccctgttt acctgtgcaa aaaaagctat
    20281 gggaatgaaa tgcttggcat agagatacag aaggacttgt acaatgtgtc tctcaagaac
    20341 aaaacaataa atgattgcag aaatgtgagt ttcatgcatg gcgatataaa ggttttggct
    20401 caattactca agcaatcacc ctttcatgtt gtggtttcaa acccccccta tacaaaagaa
    20461 aaagggggca ggaagagccc ccagcattca cggcttgttg caaggtatga aactttcttg
    20521 accctccctg atctcatgat gacaacctct tctctgcttt ttagaaaagg gagattttat
    20581 actatctatc cgtctcgaag gttgggggag ctaatcggat tcgccagact gaatagcctt
    20641 gaactgaaac gattgcgccc catctaccct agaagggaca aggaggcaaa tctctttctg
    20701 gcagagttca tcaaggaggg tggcatagga gcaaaaattg aacaacccct ttacattttt
    20761 gacaatagcg gctacactga tgaggtaaaa aaatattata ttcttaacgg ctgacaatgg
    20821 aaacagtaac ccaactcata gcagacgatc tggcaagact cgagtcaaca atccagaagc
    20881 ttataacaac gaagataggc ttcatcaaag aaattgtgaa tcacatcatc agatcaggcg
    20941 gaaaaagggt gcggcccatt ctaataatac ttactgctcg gctttgcgga tacaagggtg
    21001 aaaaacatat cccatatgca gcaataattg aattcatcca tacagcaacc ttgctccatg
    21061 atgatgtggt ggataatgct cagaccaggc gtggttcttc aaccgcaaac accttgtggg
    21121 ggaatgaacc gagcgttctt gttggcgatt ttcttttctc caagtcattt gaattgatgg
    21181 ctcataatgg gaacgaggaa atcatgagga tcatgtctga ggccacaaca tccctcgctg
    21241 aaggagagat tttagagctt ctcaaaacgt gtgatattga cacaacagag gaggaatact
    21301 tcgaaatcat caagaacaag actgcgtctc ttttttctgc cgcttgcgaa gtaggggcaa
    21361 tccttggccg ggtaaatcag acaaagaggg ctgcattacg agatttcgga tacaaccttg
    21421 gcatggcttt tcaacttact gatgacgttc tcgattatac ctcctacgat gctgttcttg
    21481 gcaagcgcgt gggcacagat ctcaaagagg gaaaggtaac ccttcccctt atccatgcgt
    21541 tggtatcctc tgctgaaaag gaaaaagatt acattaatac agtaataaac aatacaaagg
    21601 taactgcaag agactttgtc agggtcggca agatcataaa gaaatatggt ggggttgaat
    21661 atacaacgaa tgcaacacgc caattcattg aaaaggcaaa gctaaatctt caggccttta
    21721 aaccgtcagc ctataaggaa agccttgcta cactcgcaga ctacattctt ggccgggaaa
    21781 cgtaaaatcg taacgcgtga tctgcgaagc gtaatgagtt tccttccatc tgattctttg
    21841 aatcattgat ctccgtggtt tttactgatg gcgcgttacg cgttatgact tcgtgagaat
    21901 ggcaccttta ggaattacaa ctatacccct gtcggatacg aaaaactgtt tcctgtcctt
    21961 ttccaggtca tatccgattt tcatcccatc agggaccctt acatctttat caatgatcgc
    22021 cctcttcact tttgcccgca taccgaccac tacattgtga aagaggatcg atttatctac
    22081 ttgcgcgtag ctgttaaccc ttacacccgg tgacaatata cttcgctcaa cccgcccgcc
    22141 acttatgatg acaccgctgc agatgatcga atcaagtgct ttgccggccc ttcctttgtc
    22201 ctcatcggca aatactgttt tagccggagg gtactgacct tcatatgttc gtattggcca
    22261 gcttttatca taaagattaa atatggggtt tactgatgca aggtccatgt tagcctggta
    22321 ataggcatct atggttccaa tatcgcgcca gtagtcagca tcctttccac caaccatgcc
    22381 aaatctgtaa acaaacaccc tgtggtcagg atacataaac ggaataatat ctcgtccaaa
    22441 atcatggtga gaggtttcca tagcagcatc tttattcagg gtatcgatca gtgcatttct
    22501 gttgaaaata taaaccccca tggagacata tgccttaccg cgcttgcccg gaatcgcttt
    22561 cggcttttca ggtttttctt caaaacccac tgccctgccc gtctcatcaa cctcgataac
    22621 cccgaaacga tgggcctctc tcaaatcaac ttcaatcgcg gagattgtga gatcagcttt
    22681 ctttttctgg tggtatcgaa tgaaatggcc ataatccatc ttataaatgt gatcacctga
    22741 taggatcaag atatattctg catcccactc ttccagatga tacagattct gatatactgc
    22801 atctgcagtc cccttgtacc agtcctctcc aacacgcatc tgaggtgaaa gatgggacat
    22861 aaattctcca agttctgcac tgaaaatact ccatgcatca attgtatgtc gttgaagaga
    22921 gaaagatttg tactggggga gaataaagat tcttctcaca cccgaattaa tgcaattact
    22981 gagagtaaaa tcaactaccc ggtattttcc gccaaaagga acagcaggct ttgcgcgatc
    23041 tctcgtgagc gggtggaggc gggcacctac gccgcccgcc agaataaatg ctatagcatt
    23101 ctgaataatc ttcgatttca taattgtata tttaagtaat agcatattcc tgcaaataat
    23161 caatcacaaa gaaaaaattt tcttgcgctg gctggtaact tggggtaatt tcaaatctac
    23221 acgtatgaat atcttctgtg tccctccact cctgagcgcc ctagttcttt ttacactttt
    23281 ccttatgggc attatgaagg caaaggcatc gaaggccaat attctattcg ctttcatatg
    23341 cctcatcggg tgttttttaa acacagataa ggcaatcctc actgggtcgc tccatcccct
    23401 tacagctttg cgattaagca gaattgacca cgcattcctt gtttttgtga tccccctcta
    23461 ccttcatttc gctgttattg ttactggcta tcgcaggtgg cttcccggtg taaaaatctg
    23521 ttacggaata gcaatcttat ttatccctgt gacgcaacac aacctatatt taattggggt
    23581 aaaacaattc ttttttggtt attttgcatt agccgggccc ttcttctata tctttggaat
    23641 cttcagttct gcaagcatta ttctttccat atgtctcctt gtaaggaacc tgaaagagga
    23701 aaaagtggct attaaaaaaa caaggatcaa gtatatcctt gtcagttttg gttttgcagc
    23761 tgctgtcacc cattttgaca tagtcaccat gatgggctac gaatggtatc ctctgggcaa
    23821 ctttgcgttt atccctatgt cccttctcgg atatgcaatc ttcaggcatg acatcatgga
    23881 atggacaatt tttcttaaca agggcatggt tttttttacg ctgttcttta tgtccatagg
    23941 ctttttcctc ggtactacag tgattgtgag gcacgtactg ggacaaaact ttgacaggga
    24001 tcttatctct ttaatatcaa tgatcatcac cttctttttg ctgtatgtat cgagccaaaa
    24061 ggtttacagt accatcacac agtttttaca gcaggaattc attctgaaca gaacggcctt
    24121 aaggggttta agctcagaaa ttctaaccct tttaaagata agcgatataa agacaaagat
    24181 tacggttgaa ctttccagga tctttgggct ggaaaaatgt gagattaaag cagtttcgag
    24241 gattgatgca ggcgaaaatg caatactcct tgatgagcag gattcttact ggattcaggg
    24301 ttttagacta tcaatcgcca ttccatcaaa atcaaacccc tcataccttc tcctcggaga
    24361 aaagcataca gtaaacctgt acacaactga ggaaaccgag gttcttataa tgcttgccaa
    24421 caatgtggcc cttgcatttg acaatgcaga tgcctataca aaattgcagg aattctctgc
    24481 ctccctcgag caactcgtta atgaacgaac cagggcactt atccaaagcg agagccttgc
    24541 tgctgttgga aggcttgcgg caggagttgc ccacgagctt aacaatcccc ttgcgagcgt
    24601 aatgagcact atcgaatatc agattgatca tttaacagac aatgaacaaa tgcgcgaaga
    24661 cctggaattt tcattacagg aactaaggag agctgcggat atcgttaaaa gcctgctcga
    24721 tgcatctcgt cagaaagagg aaacaaaaga actcatcgat ctgcacatac ccattgagga
    24781 cgccctgaag gtcctttaca atcagtacaa aaacaagaaa ataactgtta tccgcaaatt
    24841 tgatgcagag cgcagcttcg tgaggggtaa tacttcaagg ctttgccagg tattcataaa
    24901 cctcataaaa aatgcaatcg atgcgattgg agaaaaagag ggtcatatta ccatcgaaac
    24961 cgcaaacaaa gaacttccct tgggaaataa ttaccgcggt gtcaactcag gtgatgtgat
    25021 tgtctgcaaa atcatagatg acggtgaagg gatcaatgag aaaaacattc ataatatatt
    25081 taaaccattc ttcacaacaa aatcccaggg gaaaggcata ggtcttggcc tgttcattgt
    25141 tcatgagatc gtgaaagacc ataaaggctc gattacggct gatagcaatc cgggaagggg
    25201 cactgtcttc actcttattt tcccatccca tcaacaatca tgaggtactc acctttgagt
    25261 tctatatcgt ttaacgtggc aataaggtca tcaacagacc cccgcagtac ctgctcatag
    25321 attttcgtca tctccttcac aaccaccaca ggcctgtttc caaattctac tcgcacgcag
    25381 cggagtgtct caagaagctt gcgaggcgat tcaaaaaaaa ttattggata gggaaaagag
    25441 gaaagatccc gcagggcttt tcgcttcttt cctggtcgtt ggggcagaaa accgtaaaag
    25501 aaaaacctat caacataaag gccgcagatt gaaatggccc ctgtagctgc cgatggtccc
    25561 ggtacggtct tcacttccac accttcctca tagcaggccc tcacaacaaa tccaccggga
    25621 tctgagatac acggagtccc tgcccctgat atgaggacgc cagactttcc tgaaccaatc
    25681 tgccgtgcaa tgctttttgc cctcctctct tcgttgtaac tgtttatagt aataattggt
    25741 ttatggatgg aaaggtggtt caacagtttc aatgcccgtt ccctgttttc tgccacaatc
    25801 aggtcagcat ctttcatgac ctcaatggcc cgtaacgtaa tatctttcag attgccaatg
    25861 ggcgttccga caacatacag tacaccatcc atgtccagct ccctttttga tggatctttt
    25921 ctttagcgta tcacaatagc aaagcaatag gctataggtg actgcttatg aaaaacagag
    25981 aaaaggcttg tagcaaacat tttttgtttc tcatctctta ccggttgcca aaacccatgg
    26041 cactcatccg ctttgtgtgt ttcatatcat ttactttata tctaggcgaa tattaaactt
    26101 tgaaagtatt ttgcgataaa ggtattgctt gtaaaa
//
LOCUS       DAOOMC010000024        25649 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdus sp. isolate AMR_MDS_2685
            NODE_1790_length_25649_cov_6.009299, whole genome shotgun sequence.
ACCESSION   DAOOMC010000024 DAOOMC010000000
VERSION     DAOOMC010000024.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864760
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdus sp. (wastewater metagenome)
  ORGANISM  Syntrophorhabdus sp.
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae; Syntrophorhabdus.
REFERENCE   1  (bases 1 to 25649)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 25649)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,729
            Genes (coding)                    :: 2,708
            CDSs (with protein)               :: 2,708
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 7 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25649
                     /organism="Syntrophorhabdus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1790_length_25649_cov_6.009299"
                     /isolate="AMR_MDS_2685"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2699756"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..860)
                     /locus_tag="PK125_03935"
     CDS             complement(<1..860)
                     /locus_tag="PK125_03935"
                     /inference="COORDINATES: protein motif:HMM:NF036634.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB37291.1"
                     /translation="MIDNNVISKAMAISLDKVVKELPPVPSFAEGIRRAPKRQAALSR
                     EDTILALKNALRYVPEKWHEELASEFLNELLTTGRIYAYRFRPHGQISGNPIDSYTGQ
                     CIEGKAFQVMIDNNLDFNVALYPYELVTYGETGQVCQNWMQYLLIKAYLQALDQDQTL
                     VVESGHPLGLFRSHPRAPRVIITNALMVGCFDDQENWSRAAALGVANYGQMTAGGWMY
                     IGPQGIVHGTYSTILNAARLKFDLSGDADMSGHLFVSSGLGGMSGAQGKAVEIANGVA
                     IIAEVDQSRI"
     gene            complement(928..2166)
                     /locus_tag="PK125_03940"
     CDS             complement(928..2166)
                     /locus_tag="PK125_03940"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPB37292.1"
                     /translation="MKSFGIFCTVSALFIFSMFYRVSNAVIAPDLVETFHLNAEGLGV
                     LGGAFFYSFALMQIPMGLLLDRVGPRYIMTVFGLVGACGALVFAMANTFFFAMAGRVL
                     MGMGMASVLMGSFKVFVLKFPSSRFSTLSGALISIGTLGSMLATSPLAWLSQNIGWRL
                     TFVIAGCTTIMCAFLIFWALRNKDIQQGASGNPVNENDKKIPIKNILHLVVGSLSFWQ
                     ISIFAFFRYGTFVALQGLWLGTYLMDVKGFSPVYAGNILMMLSIGYIAGAPIAGYLAD
                     KVIRSAKITAFCSVTFYTISLLPLTGIVDIKNPVIFGSLFFLIGFFNSPGTLAYTHVK
                     ELYPIHISGTVIAAVNFFVMAGGAVLTPVLGMIIAACTQKNQLSSADAYHYAFLVCFL
                     GIATSLIFYAFSKSRQVISD"
     gene            complement(2194..3582)
                     /locus_tag="PK125_03945"
     CDS             complement(2194..3582)
                     /locus_tag="PK125_03945"
                     /inference="COORDINATES: protein motif:HMM:NF024927.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HPB37293.1"
                     /translation="MSGIFGIVSKKNCASNLLYGTDYHSHMGTEYGGMAVLGKKFYRG
                     IHNISKSQFKSRFFDEYKEMDGNFGIGVISDRDAQPLIIGSKFGTFAIVSAGLVENTS
                     ELAGELLAQGETFGEMSSGGINSVELIAKLINQGRTLIDGIEGLYDKISGSASILLMK
                     EDGIYAARDRLGRTPLVVGERDGDYAIATETCSFLNLGFRIVKYLEPGEIIHIGKDGL
                     EEKLQGNQTNQICSFLWIYTGYPASSYEGISVELVRERCGRLLARNDNVQADLVAGVP
                     DSGVGHAIGYAMESGLPYRRPLVKYTPGYGRSYTPPAQEIRDLVATMKLLPVKEVIAG
                     NRIVLCEDSIVRGTQLKKYTLKKLWEAGAKEIHVRPACPPLMFPCKFALSTRSLDELV
                     ARHAIRALEGFDKEDVSEYLDDRSDKYEKMVEWIGHMLDATTLRYLRINDMVEAIGLP
                     RGKLCLHCWLGE"
     gene            complement(4158..4511)
                     /locus_tag="PK125_03950"
     CDS             complement(4158..4511)
                     /locus_tag="PK125_03950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB37294.1"
                     /translation="MFQLNGEKNYKEDNHDISEYSYPHRGFVFEQQDAKGCKIILCFR
                     KWKGSDCAFSKYIDFYTVDFINPMTQQMVTTKKRNFTKNARRLNHSYEIFLGSRYPDY
                     GNAIKKIGKQCLKKP"
     gene            complement(4748..5026)
                     /locus_tag="PK125_03955"
     CDS             complement(4748..5026)
                     /locus_tag="PK125_03955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB37295.1"
                     /translation="MSVEKSKAKTKTKKTATTKISEKTVIEAMANLEKLAKDYIGQAK
                     NEIKTGRKIADKIREVADNIENELVMEGINLTEAYDSKDFVGVGIGVL"
     gene            5409..5825
                     /gene="rnk"
                     /locus_tag="PK125_03960"
     CDS             5409..5825
                     /gene="rnk"
                     /locus_tag="PK125_03960"
                     /inference="COORDINATES: protein motif:HMM:NF004396.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside diphosphate kinase regulator"
                     /protein_id="HPB37296.1"
                     /translation="MEAKNVYITKHDLERLKKLMNDHSWEIHGKDFSIHELTDEMNRA
                     LVVSPKEIPENVITMNSRVLLREVESGRETIFWLVFPDKVSAVKNPASILSPLGTAMI
                     GHKTGDIFTWESPEGAKQMEVLDILYQPERVGNFGL"
     gene            complement(5828..6583)
                     /locus_tag="PK125_03965"
     CDS             complement(5828..6583)
                     /locus_tag="PK125_03965"
                     /inference="COORDINATES: protein motif:HMM:NF014035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TSUP family transporter"
                     /protein_id="HPB37297.1"
                     /translation="MEIILCTFAFLAGFIDSVAGGGGLIQLPALLIFLPHVPVVHLLG
                     TNKLASIAGTSNAVIQYARHVGINWLATLPATISAFALSFLGARTAATLNTEIMRIAV
                     LILLIAIAIFTFLKRDFGMVHAPKLTHIVQFSMSIGAGMIIGFYDGFFGPGTGSFLIF
                     VFVGIFGFNFLSASASSKLINFATNLSAMLYFAFTDNILYSLAIPMAVFNIIGSIAGT
                     RLAILKGNRFVRFIFLLVVAGMIFKVAYDTVVP"
     gene            complement(6767..7612)
                     /locus_tag="PK125_03970"
     CDS             complement(6767..7612)
                     /locus_tag="PK125_03970"
                     /inference="COORDINATES: protein motif:HMM:NF016269.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-binding SYLF domain-containing protein"
                     /protein_id="HPB37298.1"
                     /translation="MKRKIEFWRTRGTTLVFSVIILSLSIFPGILVTHAVADDAMDAR
                     HLADKARLTLETFMQAPETQAFRNLVVNARGVFIAPQILRGAFVVGGSGGSGVYLVRD
                     MRTNSWAGPAFYTLGGVSFGFQIGGEASEVVFLAMTERGVNALLKSGVKLGADVGVAV
                     GPVGLGADASTANLSVDILTFSRSKGLYGGISLAGSVVYVRDDWNTAYYGLNVVPSDI
                     LVSKTVSNRHALGLLNLVARATALGGQDVKVPETKSPESRAQESNPSLERDAPVQVED
                     IDIKK"
     gene            complement(7951..8175)
                     /locus_tag="PK125_03975"
     CDS             complement(7951..8175)
                     /locus_tag="PK125_03975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB37299.1"
                     /translation="MDYGSFIVRCPQCGTKNRVPNKRTTGNIVCGKCKSQLDLTLLFP
                     HGFLRVNDSSFYDEVIRFNGPVLVDFTAPW"
     gene            complement(8552..9022)
                     /locus_tag="PK125_03980"
     CDS             complement(8552..9022)
                     /locus_tag="PK125_03980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB37300.1"
                     /translation="MDLIDHDTPDAERVIYRARLHWAVLLGPILVIIIGGLALRSQGH
                     HAMVLVTFGLIWGIASYVSLQRSEIGLTTKKILVNVGYPLKRSHEIALGKIVLVDYYQ
                     PSLGAMLNFGKIIIAYDGKKKSAIRLVSHPAEFVREVQQQIVASKHVSTGPQSL"
     gene            complement(9065..10372)
                     /locus_tag="PK125_03985"
     CDS             complement(9065..10372)
                     /locus_tag="PK125_03985"
                     /inference="COORDINATES: protein motif:HMM:NF018513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HPB37301.1"
                     /translation="MSDVAIGTYGIILLLVLFLTGLEMAYCMILVGFLGFTFLMSFSA
                     ASNLVIKDFFDNFTTYSYTVIPLFMVMGEFASNSNIAKRLYLGAHKWFGHIPGGLAMT
                     TVVGATAFKAMCGSSLATVGTFSNLAMPEMDRYGYKKELSAGTIASVSTLGMILPPCT
                     VLIIYGLQVEQSIGRLFLAGIVPSLVIAALFMTVIAGWVSVKPAIAPRAEKATWKERF
                     AVIPDAVIVGIIFGIVIGGMITGFFSPTESGTIGTVAIFFLALARREVNIGMIIKSFK
                     GALKTSIMTLMLIAGSAIFGHFLAITEIPMIAAKWASGLAVPGFFVMVIIVAIYLIGG
                     SIMDDLAFMVLATPIFFPTAVELGYDPIWFGILICITLMIGGLIPPVAIYVFILGNIT
                     GIPFKTIYKGVIPFLLALIVALILLFTFPQIALWLPNLMMGKA"
     gene            complement(10365..10853)
                     /locus_tag="PK125_03990"
     CDS             complement(10365..10853)
                     /locus_tag="PK125_03990"
                     /inference="COORDINATES: protein motif:HMM:NF016200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease subunit"
                     /protein_id="HPB37302.1"
                     /translation="MMKRFVEGVAIVDKLLYIIAGSVLACMIILTFFDVILRNLGHPI
                     TGSMEIIQYGGAIVFGFSVPYGTMLGAQVIVDIVTEKLSPKKKRTIEIITRCVGVLMF
                     LFVAYNFFIYGIDVQKTGERTASFKIPYYPFAFALSFSFFLQSFTIFCDLIKKVKGAE
                     NE"
     gene            complement(10964..11986)
                     /locus_tag="PK125_03995"
     CDS             complement(10964..11986)
                     /locus_tag="PK125_03995"
                     /inference="COORDINATES: protein motif:HMM:NF015445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HPB37303.1"
                     /translation="MKKSTFFALFVFTIIFLFFLTSIPAQAQEKAMKLRLSTIWPTQH
                     PLTKLFADWGKDVEKATNGRVTVTVFAANTLSPPMQVYDNTVKGVVDVGTNLLAYSPG
                     RLPLSEVLQQPLGYKNGYQASKAANEFYKKFKPKEFDDVKVMFLHGAAPGFIFTKNPA
                     KSLADLKGLRIKANAENADIVKNLGASPVTMPVTETYDALSRGVVDGTLFPLEALQGF
                     KIGEVIKTVIEDYPMSYMTSMYCIMNKDKWNAISPADQKAIEKLNDEYNEKFGKKWVE
                     LDAKAVEFAKSKGVTFVKASKEDEALTAQRMKPILDDYVKMTKAKGLPGDEALKFVQD
                     YLKTHQ"
     gene            complement(12046..13551)
                     /locus_tag="PK125_04000"
     CDS             complement(12046..13551)
                     /locus_tag="PK125_04000"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HPB37304.1"
                     /translation="MNVATNLESSAFYFPQQPALSETDSEITYGALNERVNCIATGLI
                     NMGIRQGEHVGICAPNSSDWIAFYFGVLKAGAVAVTYSSLLSQDELTLLVDHSKPRFI
                     FTSDDKISILEGMKGPAGLEKIISQNGDINIQQIVDAGIVNFKAVDRNRTDTAAILYT
                     GGTTGTPKGVMLTHENINTSAHNVAHNEHSNEKDRAICFLPFNHVFGQMHIMNATIYS
                     AGCLEILPTFDLDKILALTGSGKVTKMFAVPTVYTRLLSLDGLKKKLGNVRYCFSAAA
                     SMAAEIVRQWKEVTKMTIYEGYGMTESASAVTFNHYYRHVVGSIGTAVPGVEVQIRDM
                     EGHRLDQGLEGEICIFGHNIMRGYLNNPEATREAFWGDGWFKSGDIGVVDKDGYLYIV
                     DRVKDMIITGGENVYPREVEEVLYMRPEVEECAVVGFPDKEWGEKVTAFIVARPGQTV
                     VPHDIKSFLKTKLSPFKVPKEYIVIEDMPKSPAGKILKRELRKRFSSGKEE"
     gene            13746..14492
                     /locus_tag="PK125_04005"
     CDS             13746..14492
                     /locus_tag="PK125_04005"
                     /inference="COORDINATES: protein motif:HMM:NF019349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HPB37305.1"
                     /translation="MPSFKPIKQSRVSGEVAEQLKQSILLGQFEAGDKLPSERDLSNQ
                     FQVSRIAIREALRFLENTGFVETRQGVTGGTFVTDLSFEHLSNAFVDLFLAEKISVPE
                     LVEVRVLVEPEIARLAATKIDRKRAKLLKEAIEAEDLPIRSLGEDLDTKTSVHYILAE
                     TCGNHFLEALERSLMALTRRVVQTVDADQPFIHPAGMHRPVVEAVLAGNPDAAAAAMR
                     HHAIEFGENLINMEKAFRARKSQGAYSTEK"
     gene            complement(14626..16335)
                     /locus_tag="PK125_04010"
     CDS             complement(14626..16335)
                     /locus_tag="PK125_04010"
                     /inference="COORDINATES: protein motif:HMM:NF019157.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tannase/feruloyl esterase family alpha/beta
                     hydrolase"
                     /protein_id="HPB37306.1"
                     /translation="MKDRNKRNRRIVLTIVFLIGLCLIFGNEAAAVKPKLSCNDLSDF
                     TYPNTVITSATLVLDGQVSVAGIGPMPEHCVVTGRMNERVGTLDGKTYAIGFEMRLPT
                     QWKKRFLYQANGGIDGSVVPAYGNDLGGGPTSNGLLKGFAVISSDAGHSGEMGPFFGI
                     DPQARIDYGYNAVAQLTPMAKSLITTYYKKQKFKSYLYGCSNGGRHAMVAASRYADQY
                     DGILAGNPGFNLPKAAVAQLWGAQQFYTITTPDTNIGSSFTPTELALVGDAILAKCDN
                     LDGLEDDMVTDTIACQGVFDLALDVPTCTGDRDGTCLTADQKDVLAAIHEGAKNTTGP
                     LYTNFPWDVGIKGSNWSFWKFTASTLLDPGAVGFIFSTPPENPLTFNSLSYALNLCGT
                     GFDPDIDAPKIFTTDATYTESGMSFMTPPDLAMTGLFEHKSKLLVVHGTSDGVFSVSD
                     TINWYESFLNTWGRQANKRARLFLVPQMNHCSGGPACDQFNMLDTLVKWVEKGQAPKS
                     IVATARGTGANVVNAEVPASWAPNRTRLLCPYPAVAKYRGRGSIEDAKRFKCVIPKTE
                     PKI"
     gene            complement(16529..18037)
                     /locus_tag="PK125_04015"
     CDS             complement(16529..18037)
                     /locus_tag="PK125_04015"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HPB37307.1"
                     /translation="MNLARNLESSAIYFPDRPAIRQGSLEWTYAELNERANKVATGLM
                     KLGVKPGDYIGLCSLNSADWIAFYFGVLKIGAVAITLSGLLTGDELAFLITHSKPKVL
                     FTDPSKLEDVMRLKSDNGLQTVICSDGDMDLSHLMRMGERAFQAIDMDRLDTAAVLYT
                     GGTTGAPKGVMLSHEGMNFAARAIAYYEHSVPTDQALCFLPFNHVFGQNHIMNSTIYS
                     SGCLELLPAFDMDRVLEAIAAGRVTKFFAVPTVYVRFLSLPDLEKKVGKLRYCFSAAA
                     SMAREIVKQWKERTGVTIAESYGMTEVMPTTFNHFYPEFHVVGSVGPVVHGVEAQIRD
                     VEGNVLSQGQEGEVCIRGRNVMKGYLNNPEATIAGMWNGEWFRSGDIGVLDDRGYFYI
                     VDRLKDLIITGGENVYPREVEEALYTQPGVEECAVIGLPDKEWGEKVSAYIVPKPGQQ
                     ISTNELKAGLKTLLSPFKVPKKIEIVKELPKSPAGKILKRELKRQVLEESKH"
     gene            complement(18830..19219)
                     /locus_tag="PK125_04020"
     CDS             complement(18830..19219)
                     /locus_tag="PK125_04020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB37308.1"
                     /translation="MLSIGEHTSDAGLLFPTKQWSRSRIDSSVCSKERLQDQPYSLPQ
                     AKAVKVVTSIHEGSTYKQIIKGGNVQGVGLIVNVSLGQSGSAKYLIASVREMYSKVII
                     NRIDDKMNGIARLAQDFKKVMHIRGQG"
     gene            complement(19840..20805)
                     /locus_tag="PK125_04025"
     CDS             complement(19840..20805)
                     /locus_tag="PK125_04025"
                     /inference="COORDINATES: protein motif:HMM:NF015679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HPB37309.1"
                     /translation="MKKAERDIHKKQSETHKHPPSLFVCIIITSILMCGWGCLADELQ
                     ARSIFQEANDFFRQGDFQASLNTYQQILEKHPMEGDRILFQMGIIYSYPANEQKDYQK
                     SLECFQKLIENYPESRYKRDSEMMIFNINNVVMKDKAIDRQKTQIESLQQRVRSREDE
                     ITALKKEIKVLEQKIFALRKEPADKVLIEKRERRLTLLSKGEILKTYKIALGGNPIGP
                     KERQGDSKTPEGVYIIDSRNRNSQYHLSLRISYPNEKDKKRAKELGVSPGGDIMIHGI
                     KNGLSWVGDLHTGVDWTKGCIAVSDEEIEEIDGLVPIGTIVEIRP"
     gene            complement(21024..21326)
                     /locus_tag="PK125_04030"
     CDS             complement(21024..21326)
                     /locus_tag="PK125_04030"
                     /inference="COORDINATES: protein motif:HMM:NF040598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lpp/OprI family alanine-zipper lipoprotein"
                     /protein_id="HPB37310.1"
                     /translation="MKKGLLAITMVIIFAISMMGCATKGDLERVQEQEMQNRAKADQA
                     AMDAQAAKAAADAATMKANEAAIRAEEAVKKAEEREKIADEKAAKADAAFQKSMKK"
     gene            complement(21715..23031)
                     /locus_tag="PK125_04035"
     CDS             complement(21715..23031)
                     /locus_tag="PK125_04035"
                     /inference="COORDINATES: protein motif:HMM:NF015679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HPB37311.1"
                     /translation="MGRTNSLFCSSGCLWLIVGSLFILSFHGCAVIKGLQKKAEPPPI
                     YPEHYLDRKDFFVANGDSVVGRLAAIKLKNGDTLPDIARHFSLGINALIEANPGVDVW
                     VPKAGERIMLPLSFILPDFVGKGIVINLASMRLFHFKGENNAVVVSTYPVGVGTTERP
                     TPMGEMHVARKVTKPTWHVPASIAEDHRKKGDPLPAKIPPGPLNPLGEYALYLSKPTY
                     LIHGTNKPSSIGLRATNGCIRLYPEDIRQLYADTPLKTPVSIINQPYLAGQRGEVLYL
                     EAHAPFESSGSGDLEKIHVKLRKMEKETGRMLDWKKVKEVLAEARGIPIPISEIREDN
                     REHIMDTIEIRHPDKLYGKPEIPELKVDAWYVLAATVSEKIEAMRLAAIINHQGPQIP
                     ATVLSINDRHRVMAGPFRDVREAKDAMKRLKIDLELDGILVEPVKK"
     gene            complement(23306..23758)
                     /locus_tag="PK125_04040"
     CDS             complement(23306..23758)
                     /locus_tag="PK125_04040"
                     /inference="COORDINATES: protein motif:HMM:NF017999.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HPB37312.1"
                     /translation="MIIEGKFTVKAPIQKLWDTLFDVQALAACLPGTEEIKQLDDKTY
                     EVTMKQKVGIIKVTMKGKQTLTKVEPPTHLEMEGEAEDMTKLGRMKDKVTMDLKDIGG
                     GEVEISYNIDVSMVGKLAMFGDRVMRSKATEVEREFVKNLSARLSSVA"
     gene            complement(23820..24080)
                     /locus_tag="PK125_04045"
     CDS             complement(23820..24080)
                     /locus_tag="PK125_04045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB37313.1"
                     /translation="MSEQQSKPVICPVCGKKAKTGSAIDCARHMFGTGDKPHRQWVDE
                     HVKEHGESFIDLLIEQATTPGNRSYVLLAEIIEKAVKEAEGK"
     gene            complement(24096..>25649)
                     /locus_tag="PK125_04050"
     CDS             complement(24096..>25649)
                     /locus_tag="PK125_04050"
                     /inference="COORDINATES: protein motif:HMM:NF014758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HPB37314.1"
                     /translation="GKFELDAAQFCACVLSMKVRKPVKIVFTREEDFIASKRRTPMFY
                     YTRTGVKKDGTFCAREAKVFTNGGAYTGMGATALYLTGFFHSFPYRWKGYRYDGYRVY
                     TNTLPSTSMRGFGAPQAMWCSEQQIEWIAKDLGLDPIEMRRKNSHHEGYVVPGQATIA
                     SCGIDLCYDEIKAWIKSKGKLPKNRSIGISACGFMSGGIFNWFDTPYSFSSAVVTVNN
                     DGTVELHVGAQDIGQGSNTTMAIICAEALGVKVEDIKVHSGDTDHCPADLGAWGSRQT
                     LMTGNAVKMAAEDAKKQLLEFAYAQSGLNIVYDLDIKEGWVHAIARPERGEPFADLVK
                     RATRGKDGQRIVGRGYYTPHRKGMISPAYSYMLQGVEVEIDEETGKIKLIDSMTAHDC
                     GQPINHLGLIGQLEGAFSMAAGYGYLEYMPFEDGKMMNPNLVDYKMIRSTGMPPANIA
                     EIDTYEPEGPYGAKEAGEGLTNPTAAAIGNAIFHRFGIQMKECPVRPEMIVNALKEKA
                     EKDKEAKKK"
BASE COUNT         6946 a         6126 c         5241 g         7336 t
ORIGIN      
        1 tggattctcg attgatctac ttccgcaata atagcaacac cattcgcaat ttctactgct
       61 tttccctggg ctccgctcat acctccgaga ccggaagaga caaaaagatg tccgctcatg
      121 tcagcatcgc cagaaaggtc aaactttaac cgtgccgcat tgagaatggt gctgtaggtt
      181 ccatgaacaa tcccttgagg gccgatgtac atccagcccc cggcagtcat ctgaccatag
      241 tttgccaccc cgagcgctgc agcacggctc cagttttcct ggtcatcaaa gcatccgacc
      301 atgagggcat tggtaatgat gacacggggg gctctgggat gtgaccggaa gaggcctaac
      361 ggatgacctg actcaacaac cagggtctga tcctggtcaa gagcctgaag atatgccttg
      421 atgaggagat attgcatcca gttctggcac acctgcccgg tttctccgta tgttaccagc
      481 tcataaggat agagtgctac attaaaatcc agattgttat cgatcataac ctggaaagcc
      541 ttgccttcta tacattgacc agtatagctg tcaatggggt ttccactgat ttgtccgtga
      601 ggtctgaacc tgtatgcata gatacggcct gttgtgagga gctcattcag aaattcggag
      661 gcaagttctt catgccactt ctctggaaca tatcgaaggg cattcttcaa tgcaagtata
      721 gtatcttctc tgctgagagc agcctgtctc ttcggtgcgc gcctgattcc ctctgcaaaa
      781 gaaggtacag gtggaagttc ttttactact ttatcaaggc ttatagccat ggcctttgag
      841 ataacgttgt tatctatcat aattgcaccc ccttatataa catcaaaatt ttaactgttg
      901 aagaggatga ttacaacatt ttttacatta atcactgatt acctgcctgc ttttcgagaa
      961 agcatagaat atgaggcttg tcgcaatacc cagaaagcag accaagaaag cgtaatggta
     1021 ggcatctgca gatgacaact ggtttttttg cgtacaggct gcaattatca tccccaggac
     1081 cggtgttaaa actgcgcctc ctgccatgac aaaaaaattc acggctgcga ttacagtacc
     1141 ggaaatatga atggggtaaa gttcttttac atgggtatat gcaagggtgc cagggctgtt
     1201 aaaaaaaccg atcagaaaga aaagtgatcc gaagataact gggtttttaa tgtctacaat
     1261 accggtgagc ggcaatagac tgattgtata gaaagttaca gaacaaaaag cagttatttt
     1321 tgctgatcgt atcactttgt ctgcaaggta gccagcaatc ggggcaccgg caatataacc
     1381 gatggacagc atcatgagga tgttccctgc ataaaccggg gaaaaacctt tcacatccat
     1441 gaggtaggtt cccaaccata acccctgcaa agcgacaaag gttccataac gaaaaaaagc
     1501 aaaaatactg atctgccaga aagagagact tcccaccaca aggtggagga tgttttttat
     1561 cggtattttt ttgtcatttt cattgactgg gtttccggag gctccctgtt gtatatcctt
     1621 gttcctcagt gcccagaaaa tgaggaaagc acacatgatc gtggtacatc ccgcaatgac
     1681 aaaggttaat cgccaaccga tgttttgtga aagccatgca aggggtgatg tagcaagcat
     1741 ggaacctaga gtgccaattg aaatgagggc accggaaagg gtggaaaacc tgctacttgg
     1801 gaattttaag acaaaaacct tgaaggagcc catcaacaca gaggccatac ccatacccat
     1861 gagaaccctc cctgccatgg cgaagaaaaa cgtgtttgcc atggcaaaaa caagggctcc
     1921 acaagcgccc accaagccaa agactgtcat gatataccgt ggccctaccc ggtcaaggag
     1981 gagacccatg ggtatttgca taagagcaaa ggaatagaag aatgcaccac cgagtacgcc
     2041 taggccctcg gcgttcaggt ggaaggtttc cacgaggtcg ggtgcaataa ccgcgttgga
     2101 tacgcgatag aacatagaaa aaatgaacag tgcagagact gtgcagaaaa ttccgaacga
     2161 tttcatgggc taccccaggg aagagtggag cccctactcc cctagccagc aatggaggca
     2221 gagctttccc ctaggaaggc caatggcttc caccatatcg tttatccgga gatatcgaag
     2281 ggttgttgca tccaacatgt ggccaatcca ctcaaccatc ttttcgtatt tgtctgaccg
     2341 gtcatcgaga tattcgctca cgtcctcttt gtcgaaacct tcgagagcgc gtatggcatg
     2401 gcgcgcgacc agttcatcca gcgaacgggt tgaaagagca aatttgcatg gaaacatcaa
     2461 aggcggacat gcaggacgta cgtgaatctc ctttgcccca gcttcccaaa gttttttaag
     2521 ggtatacttc ttgagttgcg ttccccgaac aatggaatcc tcgcagagaa caatcctgtt
     2581 ccctgctatt acttccttca ctgggagaag cttcattgtt gcaacaaggt ctcgaatctc
     2641 ctgtgcagga ggtgtgtagc ttcttccata accgggggta tacttaacaa gaggacggcg
     2701 gtaaggaaga cctgactcca tggcataacc aatcgcatga cctacccctg aatcagggac
     2761 acctgcaaca agatcagcct ggacattatc atttctcgcg agaagccttc cacaccgctc
     2821 tcgtacaagt tcgacgctga tgccttcata gcttgacgca gggtatccag tgtaaatcca
     2881 caaaaaggaa cagatctggt tagtctggtt tccctggagt ttttcctcca gcccatcctt
     2941 tccaatatgg atgatctcgc ccggttcaag atactttacg atacggaatc caaggttaag
     3001 aaaagagcag gtttcagtgg caatagcata gtctccatcc cgctcgccta ccacgagagg
     3061 tgttctcccc agacggtcac gggcagcata aataccatct tctttcatta gaaggattga
     3121 ggctgacccg ctgattttgt catacagtcc ctcaattccg tctatcaaag ttctcccctg
     3181 attgatcagt ttggcaataa gctccacgga attaatgcct ccactcgaca tctctccaaa
     3241 ggtttctccc tgggcaagaa gttccccggc aagttcgctt gtattctcca caagacctgc
     3301 tgacacaatg gcaaaagtgc cgaactttga accaattatt agaggttgag catcccggtc
     3361 gctgattaca ccaataccaa aattaccatc catttcctta tattcatcaa aaaatcttga
     3421 tttaaactgc gatttgctga tattgtgaat accacggtaa aattttttcc caagaacagc
     3481 catgccaccg tactctgttc ccatatgaga gtgataatct gttccgtaga gaaggttact
     3541 tgcacaattc ttttttgaca caatcccgaa gatgccgctc atgagtcctc ctagagttcc
     3601 gatgccttag atttttcgac tgttaatgaa atggatcagc ttcaaacatt gaatatgata
     3661 caaactctcc atgtttttct caagcaataa accatgaaat tcatggcaaa gatcggttgt
     3721 caggattttt ttgttttgtt cttgctccac aggcttaacc ctgtgattct ttgcataacc
     3781 gccttctgtc ttgttctggg tacccggttc acgttggcct atcacgtggt ctttgagaca
     3841 ctggggtcta ccttgtattt ggcagttaga accttggcca cagcacctgc ttatgggtgg
     3901 gaaagcttca tattgtgatc cacactgccc aatgttgtcc atgtgacctc tataaatatt
     3961 ttgaggctgg gattgctcga aaggagttta ttcatttcct gaagacagga tcatattaaa
     4021 gctccgcagc tgatttatta aaatcgaagt agttatccac aaatggccag atttaatctt
     4081 cttcaaaatt aaactagagc tttgcccaaa tctctttgct gtcttatatg agtttcattg
     4141 atgcttcagg ccaatcctta gggttttttt aaacactgtt tccctatctt tttgatagca
     4201 ttcccataat cgggatacct ggagccgaga aaaatttcat acgaatggtt taagcgtctc
     4261 gcgttctttg tgaaatttct tttctttgtt gtaaccattt gttgcgtcat aggattaatg
     4321 aaatcaacag tataaaaatc tatatatttt gaaaaagcac aatccgatcc cttccatttt
     4381 cgaaaacaaa ggataatctt acaccccttt gcatcctgtt gctcaaatac aaaacccctg
     4441 tgaggatagc tgtattcaga gatgtcatgg ttatcctctt tataattctt ctcgccgttg
     4501 agttggaaca ttattgaaaa ccctacataa aacaaagccg gttctttaca aaaaaatagg
     4561 agccattaca atccattgtg ccgctgagtg cacgttaggt ttgcagtggc tcccgtgcag
     4621 atatccaaac ttcccgcctt cttactcgaa acaacctcaa cacatctaaa aattatgtat
     4681 gcattacatt aagcaataat ctatgtagct ataatatttt taatctttca acataatatt
     4741 tcctgcttca aagaaccccg atacctactc caacaaaatc ttttgagtca taggcctctg
     4801 tcaggttgat cccttccatg acaagctcgt tctcaatatt atctgccact tctcgtatct
     4861 tgtccgcaat ctttcgtcca gtttttattt catttttagc ttggccaata taatctttcg
     4921 ccagcttctc aaggttggcc attgcctcta taactgtctt ttctgatatc ttagttgttg
     4981 ctgttttctt tgttttagtc tttgccttgc tcttttcaac tgacatgtaa cacctccatt
     5041 taattttgta attggagtca tcgaaagtcg actctatttt tccctatagt tgttggattc
     5101 taaaccccaa tccacaacca agcctattat aagcatctat ttttcattat accctgaata
     5161 atgtattagt caagcacaac atgttatttt accgctacat ggaattgatc accacgaaaa
     5221 gatatcacaa acatattcac acaactctcc aaaaggactc acaaccactt gactggaaat
     5281 caggtatatt cctcacgtta ccaattcatt ttttttctat gaatgattat aatttaacta
     5341 taatcagtgt ggtggggtta tctgacagaa aaggtttcgc gttggcaaga attttcgttg
     5401 ggtgaaaaat ggaagcaaaa aacgtttaca taacaaagca tgatcttgag aggttgaaaa
     5461 agctcatgaa tgatcattcc tgggaaatcc atggcaaaga cttttctatt catgaactga
     5521 cagacgaaat gaatcgtgca cttgtggttt caccaaaaga aatacctgaa aatgtaatca
     5581 ccatgaactc cagggtgctg ttgagggagg tggagtcagg cagggaaacg atcttttggc
     5641 ttgtttttcc ggataaggtt agtgcggtaa aaaatccagc ctctatctta agcccccttg
     5701 ggacagccat gatcggacat aaaaccggtg atatctttac gtgggaaagc cctgaaggtg
     5761 cgaaacagat ggaagttttg gacatcttgt atcaacctga gcgcgttggg aacttcggac
     5821 tgtaaaatca tggcaccaca gtgtcataag caactttaaa aatcatccct gccacaacaa
     5881 gcaaaaagat aaaacgtaca aatctatttc ctttcagaat ggcgagtctc gttccggcga
     5941 ttgagccaat tatattaaag acggccatgg gaattgcgag gctgtacaaa atgttgtctg
     6001 taaaagcaaa gtaaagcatg gctgagaggt tcgttgcaaa atttattagc tttgaagagg
     6061 cagacgctga gagaaaatta aaaccgaaga tacccacaaa aacaaaaata aggaaactgc
     6121 ctgtgcctgg accaaagaac ccatcgtaaa aacctatgat catcccggca cctatactca
     6181 ttgagaattg aacaatatgg gtcagtttcg gcgcatgaac cattccaaag tctcttttga
     6241 gaaaggtaaa tatggcaata gcaattaaaa ggatcaatac ggcgatcctc attatctcag
     6301 tattgagggt tgccgctgtt cgagcaccaa ggaaggaaag ggcgaacgca gaaattgttg
     6361 caggtaatgt agccagccaa ttaataccta cgtgccttgc atattgaatg actgcattgg
     6421 aagtgcccgc aatagaggca agcttgttcg ttccgagaag atgaaccaca ggtacgtggg
     6481 gcaagaagat aagcagggcc ggaagctgaa tcaatcctcc cccaccagca acagaatcaa
     6541 taaatcctgc taaaaaggca aaagtacata gaatgatttc caaaatcggg cttgcctccg
     6601 ggtccttata tctgcccaaa gtcaggggag aacaaaattg cttttggcga gtttactcag
     6661 acatggattg tcatggggtt tttgtaacac ctttcttact tacagaagaa tattagagcc
     6721 tatgtgttac ggctcatacc acgtattttg gcaatccttt aaaatatcat ttttttatgt
     6781 ctatgtcttc cacctgcact ggagcatccc tttccaaaga tggatttgat tcctgtgctc
     6841 tactttcagg tgattttgtt tctggcactt tcacgtcttg tcctcccaat gcggttgccc
     6901 ttgcaacaag attgagaagc cctaaagcgt ggcgattact caccgttttt gacacaagaa
     6961 tgtcagatgg aactacgtta agcccatagt aagcagtatt ccaatcatca cgtacataaa
     7021 caacagaacc tgcgagtgaa attccgccat acaatccttt agagcgggag aaggtgagaa
     7081 tgtcaacact cagatttgct gttgaagcat cagcccccaa gccaactggt cctacagcca
     7141 ccccaacatc agcaccaagc tttacgccac tcttaagaag ggcattgact cctcgttcag
     7201 tcattgcaag aaagacaact tccgacgcct ccccgccaat ctgaaagcca aaactgacac
     7261 cgcccagtgt ataaaaagca ggtcctgccc aactatttgt cctcatatcg cgaacgaggt
     7321 aaacaccgct gcccccggaa ccgccaacaa caaaggcacc cctcagtatc tgtggtgcaa
     7381 taaacacacc tctggcattc accaccaagt tcctgaaagc ctgtgtttcc ggtgcctgca
     7441 taaaggtctc aagtgtaagt cttgccttgt cagcaagatg acgggcatcc atggcatcat
     7501 ctgctacagc atgtgttaca agaataccag gaaaaatgct cagggaaaga atgatgaccg
     7561 aaaaaacaag agttgttccc ctggttctcc aaaactcaat ttttcttttc atgatgactc
     7621 cttttgcata atggtaattt aacctgcaac acagagggta ctttgttgat tcaaagtata
     7681 tcaaacacgc actattgtca agaaaaggtg cttttttgat taacgtgcca ttgaattgag
     7741 atgtcgttca atctcggatt ttggaagcgc cccgacggcc tgatcaacaa ttcgaccccc
     7801 attgtaaaag taaaaagcgg gaacaccccg tatgccatga tttgaggctg ttctcggaga
     7861 ttcgtccaca ttaaccttta caaattttat ttttcctgca tactgatttg ccagttcgtc
     7921 cagaaccggt tcaagacctc tgcagtgccc tcaccatggt gctgtaaaat ccacaagaac
     7981 cgggccgtta aaacgaatta cctcatcgta gaacgaacta tcatttaccc tcagaaaacc
     8041 atgtggaaaa agaagggtta agtcgagttg tgacttacat ttgccgcaaa caatgttccc
     8101 tgttgttcgt ttattgggaa ccctgttttt ggtaccacat tgaggacatc gaacaataaa
     8161 gctaccgtaa tccatgattc acctcccatt cctttaatgt gtaaataata taaatactgc
     8221 cctggacctt tcaagcattc catgattttt tcctgttgtc catggagtat tacatgctga
     8281 aatatctctg tttatcgaat tctaccgccc tctgaatgat tctgtttttt tcaaattatt
     8341 aacaggtaac gaggcaacaa aagtcggtat tgcggctttt ggggcacctt cagttcaatg
     8401 tttttactgg tcagcttaat ttagtatgag attcctgatt gactgctctc aagacggtaa
     8461 tgtggagagg tcgaaggggg cgttcttgat gtgacctccc cacataccaa ggcgtgcttt
     8521 ttcaatgctt tctcaaccca ttggtctccc ttcacaggct ttgggggcca gtggaaacat
     8581 gttttgaagc tacgatctgt tgctgaacct ctctaacaaa ttctgcagga tgggaaacaa
     8641 gccgaatggc agatttcttt ttgccgtcgt atgcaataat aattttgcca aaatttaaca
     8701 ttgcaccaag agaaggctga taatagtcaa caaggacgat cttgccaagg gcaatctcat
     8761 gagatctctt cagaggataa cctacgttga ctaaaatttt tttggtagtt aatccaattt
     8821 ctgatctctg caaacttaca taggatgcta tcccccatat aagaccaaag gtcacaagta
     8881 ccatggcgtg atgcccttgt gacctcagcg ccaatcctcc tatgataatc accagtatag
     8941 ggccaagcag cacagcccag tgcaatcggg ctcgatagat aactctttca gcatcgggtg
     9001 tatcgtggtc aatcaaatcc atctaccgtg ttttcaatag ggtaatcaag caatatcagt
     9061 ctcgttatgc tttgcccatc ataaggtttg gcagccataa tgcaatctgg ggaaaggtaa
     9121 acaggagtat caatgccaca ataagagcta acaaaaaagg gataactccc ttatagatcg
     9181 ttttgaaagg gattcctgtt atgttcccca atatgaatac ataaatcgct acaggaggga
     9241 taaggcctcc gatcatgagt gtgatgcaaa tcaggatgcc aaaccatatt ggatcgtacc
     9301 caagttctac agctgtggga aaaaatattg gcgtagcaag taccatgaat gcgaggtcat
     9361 ccataattga accgcctata aggtatatgg cgactattat aaccataaca aagaagcccg
     9421 gtacagcaag cccactggcc cactttgccg caatcatcgg gatttccgtt attgcaagga
     9481 aatggccaaa aatggctgat ccggctataa gcattagggt cataatagaa gtcttgagtg
     9541 cccctttgaa agatttgata atcataccta tattgacttc tcttcgtgca agagccagaa
     9601 aaaatattgc cactgttcct atggttcctg actcagtggg actgaaaaag ccagtgatca
     9661 taccgcctat gacgataccg aagataatac caacgatcac agcatccggt atcacggcaa
     9721 atcgttcttt ccatgtcgcc ttttccgccc tgggagctat ggcgggtttt actgacaccc
     9781 atccggctat aaccgtcatg aagagcgcag ctataaccag ggacggcaca atccctgcga
     9841 gaaaaagcct gcctatggat tgctcaacct gaagcccata aataataagc actgtacaag
     9901 gaggtagaat catgcccagg gtgctcactg atgcaatcgt gccggcagag agttcttttt
     9961 tatacccata tcgatccatc tcgggcatgg caaggtttga aaaagtcccg actgtggcaa
    10021 ggcttgaccc acacatggct ttgaaggctg ttgcccccac tacggttgtc atagcaagtc
    10081 cgccggggat atggccaaac cacttatgcg ctcccagata aagcctcttg gcaatatttg
    10141 aatttgaggc aaactcaccc atgaccataa agagggggat cactgtataa ctgtatgtgg
    10201 taaagttatc aaagaaatcc tttataacga gattggaggc cgctgaaaag gacatgagga
    10261 aggtaaagcc aagaaaaccc acaagaatca tacagtaggc catttcaagt ccggtaagga
    10321 aaaggaccag aagcaatatt attccatatg ttccgattgc cacatcactc attttctgct
    10381 ccttttactt ttttaatcag gtcgcaaaat attgtgaagc tctgcaagaa aaagctgaat
    10441 gaaagggcaa aggcaaaagg atagtatggt atcttaaagc tggcagtacg ttcccctgtt
    10501 ttttgaacgt ctatcccata aatgaaaaaa ttatatgcaa caaacaagaa catcaagacc
    10561 ccaacacacc tggtgatgat ctcgatggtg cgcttttttt tgggactgag tttttccgtg
    10621 actatatcaa cgattacctg ggctccaagc attgtgccat acggaactga gaacccaaat
    10681 acaatcgctc cgccatattg tataatctcc atggaaccgg taatgggatg gccgagattc
    10741 ctcaagatta catcaaagaa agttagtata atcatacagg ccagaacact accggcaatg
    10801 atgtacaata acttgtcaac aatggcaaca ccttcaacaa atcgcttcat cattttcctc
    10861 cgcgcaaata catcacaact gaggcacctc tgttttttgc atcagtatca tgtatatgca
    10921 aagagggctg taatacacag ccctctttta cgaaaatata actctactga tgagtcttca
    10981 ggtagtcctg gacaaatttc aacgcttcat cgcccgggag gccctttgcc tttgtcatct
    11041 tgacataatc atcgaggatc ggcttcatcc tttgtgctgt aagtgcctca tcctccttgg
    11101 aagccttaac aaaggtcaca ccttttgatt tggcaaattc aactgcttta gcatccagct
    11161 caacccattt ttttccgaat ttttcattgt attcatcatt gagtttttct atcgccttct
    11221 ggtcagccgg ggatatggcg ttccatttat ccttattcat aatacaatac attgatgtca
    11281 tataggacat ggggtagtct tcaataactg tcttgatgac ctccccaatc ttaaatccct
    11341 gcaaagcttc aagagggaaa agggttccat ctacaacgcc ccttgaaagg gcatcgtatg
    11401 tttcggtaac cggcatggtc acgggggagg ctccgaggtt ttttacaata tccgcattct
    11461 ctgcattggc ttttattctg agtcccttta agtcggcaag cgatttagca gggttcttag
    11521 taaaaataaa gccaggtgca gcaccgtgga gaaacataac ctttacgtca tcgaattctt
    11581 tcggtttgaa ttttttatag aattcattag ccgccttgct tgcctgatac ccattcttat
    11641 atcccaaggg ttgttgcaga acctcagaaa gtgggagcct gcccggtgag taagccaata
    11701 agttggtgcc aacatcaaca actcctttca ctgtgttgtc atagacctgc atggggggcg
    11761 aaagcgtgtt agcagcaaat actgttactg taaccctccc atttgtcgcc ttctccacat
    11821 ctttacccca atcagcaaac agctttgtca aagggtgttg tgttggccat attgtcgaaa
    11881 gtctgagttt catagctttc tcttgggcct gtgccgggat tgaggtcagg aaaaacagaa
    11941 aaattatcgt aaaaacaaac agtgcaaaaa acgtactttt tttcatactg catcctcctt
    12001 tttttgtgtt tctatattgt aatctcaata accctcattt acaagtcact cctctttacc
    12061 gctcgaaaac ctcttcctta actcccgctt caatatttta ccagcaggac tctttggcat
    12121 atcttcgatg acaatatatt cttttggtac cttaaaaggg gacaattttg tcttaaggaa
    12181 agacttgata tcatgaggaa ccactgtttg gccaggtctg gcaacgataa atgctgtaac
    12241 cttctctccc cattccttgt caggaaaccc tactacagcg cattcttcta cttctggacg
    12301 catgtaaagc acctcctcta cttctcgggg atagacattc tctccaccag taatgatcat
    12361 gtctttgaca cggtcaacga tataaagata gccgtctttg tcaacgacgc cgatatcccc
    12421 tgacttgaac cagccgtcgc cccagaaagc ttctcgggtt gcttcagggt tattcagata
    12481 tcctctcata atgttgtgcc caaaaataca aatctctcct tcaagcccct gatcaagcct
    12541 gtgtccctcc atgtcccgta tctgtacttc aaccccggga accgctgtac ctatggatcc
    12601 taccacgtga cggtaataat ggttaaacgt aacagcagac gcagactcag tcatgccata
    12661 gccttcgtaa atcgtcattt ttgttacttc tttccattgc cttacaattt cggcagccat
    12721 acttgcagct gccgaaaaac aataacgcac attccctaat ttttttttaa gaccatcaag
    12781 actaagcaga cgggtataga ccgttggaac tgcaaacatt tttgttacct taccagagcc
    12841 agtaagggcc aggatcttgt caagatcgaa agtaggaaga atttcaaggc aaccagcact
    12901 gtaaattgta gcgttcatga tgtgcatctg cccaaaaaca tgattgaagg gtagaaaaca
    12961 gattgccctg tccttctcat ttgagtgctc gttgtgcgca acattatgtg ccgatgtgtt
    13021 gatgttttcg tgggtcagca taacgccttt gggcgtccct gttgtaccgc cagtgtatag
    13081 aattgcagct gtatcagtgc ggtttctatc cacagcctta aaatttacta tccctgcatc
    13141 tacaatctgc tggatattga tatctccatt ctgactgatg attttttcca gacccgcagg
    13201 tcctttcatg ccctcgagga ttgagatttt gtcatcagat gtaaaaataa accttggttt
    13261 tgagtgatct acaagcaatg ttagttcatc ttgactcaag aggctcgagt atgtgactgc
    13321 cactgcgcct gccttgagaa ccccaaaata aaatgcaatc cagtcggacg agttgggggc
    13381 acaaattcca acgtgctcac cttgtctaat tcccatgttg atgagtcccg ttgcaataca
    13441 attaaccctt tcattcaacg ccccgtatgt tatttcagag tctgtttcgc tcaatgcggg
    13501 ctgttgagga aaatagaatg ccgatgactc gaggtttgtg gcaacgttca tataggcctc
    13561 caaataagat tcgcaatgcg caaaccacct ggttaataag cgctgacaga aagattaaat
    13621 cgtgctcttt tctattggta taatatttag atatattaat ttattttgtc aagtgaataa
    13681 ttaaaatttt cttgcaaaag atttcgactt tgttatacta tgacaaaatc caagaggtat
    13741 catacgtgcc ttcattcaaa cctatcaaac aatccagggt gtcaggagaa gtagcagagc
    13801 aactgaagca atcaatcctt ctcggtcagt tcgaggcagg tgataagtta ccgtcagagc
    13861 gtgatctttc aaaccagttt caggtcagcc gcatagcaat aagagaggct ttgaggttcc
    13921 ttgaaaatac gggttttgtt gaaaccagac aaggtgtcac agggggcact tttgtcaccg
    13981 acttatcgtt tgaacatctg agcaatgcct ttgtggatct ttttctggct gagaaaattt
    14041 ctgttcccga actcgttgaa gtgcgcgttc tggtggagcc cgaaatagcg agattggcgg
    14101 cgacaaagat tgatcgtaag cgtgccaaac ttttgaagga agccattgaa gcagaggatt
    14161 taccgatccg atcgctcggg gaggacctcg ataccaagac atctgttcat tatatcctgg
    14221 ccgaaacatg tggaaatcat tttttggagg cccttgagag gtccctcatg gctcttacca
    14281 gacgagttgt tcagacagtt gatgctgacc aaccatttat ccaccctgca gggatgcacc
    14341 gacctgttgt ggaggcggtc ctggctggaa atccagatgc agcagcagca gccatgagac
    14401 accatgcaat cgagtttggt gaaaatctaa taaatatgga aaaagccttt cgagcaagaa
    14461 aatcccaggg cgcatattca acggaaaagt aataccttcg ctgtcaggta aattcatttg
    14521 tgatatggtg attcaactga ttgtccgatt gttgtctaat tcatgaggca ggaccattcc
    14581 taaccctgcc tcatgaatag tgaccttcgt gttatctcgc aattcctaaa tctttggttc
    14641 agtcttaggg atcacgcatt taaaacgttt cgcatcctcg atagaacctc taccacgata
    14701 cttggcaaca gcaggatagg ggcataacag ccttgtgcga tttggggccc agctcgctgg
    14761 cacttctgca ttcactacgt tggcacctgt tccacgggct gttgcaacaa tagatttcgg
    14821 tgcttgtcct ttctcgaccc atttcacaag agtatccagc atattaaact gatcgcatgc
    14881 aggaccgccg ctgcagtgat tcatctgcgg aacgaggaag aggcgtgccc ttttattagc
    14941 ttgccttccc caggtgttta gaaagctttc ataccagttg atggtatctg aaacagaaaa
    15001 gaccccatca gacgtaccat gcacgaccaa tagcttggac ttgtgttcaa ataatcccgt
    15061 catagctaga tcaggcggtg tcataaaaga catccccgac tcagtatagg ttgcatcggt
    15121 ggtgaatatc ttgggagcat caatgtcagg atcaaaacct gtgccacaca gattaagtgc
    15181 gtaggacagg ctgttaaagg taagtgggtt ttctggaggg gttgaaaaga tgaaacccac
    15241 ggcgccgggg tcaagcaatg tagatgctgt aaatttccaa aagctccagt ttgaaccctt
    15301 gataccaaca tcccacggaa agttcgtata gagcggtcct gtggtgtttt tcgcaccctc
    15361 atgaatggca gctagcacat ccttctgatc ggctgtaagg cacgtcccat cgcgatcacc
    15421 cgtacaggta ggtacatcca aggcgagatc gaatacccct tgacatgcga tcgtatccgt
    15481 aaccatatcg tcttccaatc cgtcaaggtt atcacacttg gcgagaatcg catcgcctac
    15541 aagagccagt tccgttggag taaaactgct accgatgttc gtgtcaggtg tggtgatcgt
    15601 atagaactgc tgtgcccccc acaattgggc aacagcagcc ttgggaaggt tgaatcccgg
    15661 atttccagcc aatatgccgt catactggtc agcatagcgc gatgccgcta ccatggcatg
    15721 acggccacca ttggaacaac catacaaata ggatttaaac ttctgcttct tataatatgt
    15781 ggtgatcaga ctttttgcca tgggtgtgag ttgggcaaca gcgttgtagc cgtagtcgat
    15841 tcgcgcttga ggatcaatgc caaaaaaagg acccatttct cctgagtgcc cagcatccga
    15901 gctgataacg gcaaacccct ttaaaagccc gttcgacgtg ggaccaccgc cgaggtcatt
    15961 tccatatgct ggtacgacgc taccgtcaat gcctccatta gcctggtaaa gaaatctctt
    16021 cttccactgc gtcggcaggc gcatctcaaa gcctatggcg taggttttac cgtccagagt
    16081 tcccacacgt tcattcatcc tacctgtcac aacgcagtgt tcaggcatgg gcccgatgcc
    16141 ggccacacta acttgaccat ctaacaccaa ggtggccgac gtgataactg tgtttggata
    16201 agtgaagtct gataggtcgt tacaagacag ttttggtttg acagctgcag cctcatttcc
    16261 gaaaatcaaa caaagtccta taagaaaaac aatggtcaat actatgcgcc tattccgttt
    16321 gtttctgtcc ttcatgcgtt accctccttc attactccat ataattcaca cattgtaatc
    16381 gtgtgctgaa gaatatttgc cacctgatca atccgggttc atctgtgggt ggccctcctt
    16441 ttgttttaca tcagtccgat ggtgcaaaaa acacccttca tcacctcctc aggctaacat
    16501 ttcataacaa cttgcaaccc gcccaaccct agtgttttga ttcttccaaa acctgcctct
    16561 tgagctccct tttcagaatt ttccctgcag ggctcttcgg taattcttta acgatctcga
    16621 tcttcttagg tactttaaaa ggagaaagaa gggttttgag gcctgctttc aattcattcg
    16681 tagatatctg ctgccctggt tttggaacaa tataagcact gaccttttcg ccccactcct
    16741 tgtcaggaag gccaatgacc gcacactcct ctacccctgg ctgagtatat agagcctctt
    16801 cgacttccct gggatacaca ttctcgcccc ctgtgattat tagatcctta aggcgatcaa
    16861 cgatgtaaaa atatccccga tcgtcaagaa cacctatatc ccctgaccgg aaccactctc
    16921 cgttccacat tccggcaatg gtagcctcgg ggttgttgag gtaccccttc atcacattcc
    16981 gtcccctgat gcatacttca ccttcctgac cttgtgaaag cacattgccc tccacatctc
    17041 tgatctgggc ctcgacccca tggacaaccg gtcctactga tcccacaacg tgaaattcag
    17101 ggtaaaaatg gttaaacgtg gtaggcataa cctctgtcat cccgtaagac tctgcaatag
    17161 tgacgccagt gcgctccttc cattgcttca caatctctct tgccatactt gcagcagccg
    17221 aaaaacagta gcgaagtttt cccaccttct tctccaagtc cggtaggctc aggaaacgaa
    17281 catacacagt cggtaccgca aaaaatttcg tgaccctgcc agcagcaata gcctccaaga
    17341 cacggtccat gtcaaatgct gggagcaact ccaggcaacc cgagctgtat atcgtcgaat
    17401 tcataatatg attctgccca aagacatgat taaagggaag aaaacagagc gcttggtcgg
    17461 ttggtaccga atgttcataa tatgcaattg ctcgtgcagc aaaattcatt ccctcatgac
    17521 tcaacatgac gcctttaggt gcccctgtcg tcccaccggt gtaaagaaca gctgcagtgt
    17581 ctagtcgatc catgtcaatt gcctgaaacg cacgttctcc catcctcata aggtgagata
    17641 ggtccatatc tccatcagaa caaatgactg tttgaaggcc attgtcgctc ttcagcctca
    17701 taacgtcttc aagtttgctc gggtcagtaa acagtacttt tggttttgaa tgagtgataa
    17761 gaaatgccag ctcatctccg gtcaaaagcc cagaaagagt gattgcaact gcaccaattt
    17821 tgagcacgcc aaagtaaaaa gcaatccaat ctgctgagtt cagtgaacag aggcctatgt
    17881 agtctccggg tttgacccca agtttcatga gaccggtggc aactttgttt gcccgttcgt
    17941 ttaactctgc gtaagtccac tcgaggctgc cctgtctaat ggctggtcgg tcaggaaaat
    18001 atatagctga tgattccaaa ttcctggcaa gattcattat gatgtctcct ttaaaagtaa
    18061 ttatatcaat tcaaaacttc atcaatggcc aagatggagt tagagctctc aaacttgtca
    18121 atattggtct aatatataaa tatttataat agataaaata tttttgtcaa caagtttgtg
    18181 ttatcggttc ttctgacatg catctacgct gcggtgatca agccttcaca gaaaacctgc
    18241 tggaagtgtt caaccgtatc aattggagta acagttagtt attcgagaaa tgcctacttg
    18301 cttccagtat gtaggtacca ggttttggca accacagggg ccctgttaac tttagcccat
    18361 cgttgtctac tcaaacctta gacatcacat caactttatc agaaagggtt ttcaggcttc
    18421 catccaacag ggggagaatg tactgatctc aacataaaat actcgtaaac agccacatga
    18481 tgaggaaaaa gaatagctca attttcggag gcattaatgg taattcttgc cggcccttcg
    18541 gtttagggct tactctacaa tcgcctttgt gatataagtt tttactgcaa acatttttaa
    18601 gttagcttcc ccagactttc tgatctatcc attgccaaaa tacctcacag tatgcaccat
    18661 tggctcattc agactgttca attcccgaat attatgggta tttttgcaag atatgtaaga
    18721 tatattcaag gcttaaatcc tgtcaagcag aagtttgcag agaaactgcc tgaagaaaga
    18781 gtctgatcgt tccctctgcg cagagttcag gtattcgctt aagttcagct taaccctgtc
    18841 ccctgatatg cattactttc ttaaaatcct gtgcaagcct tgcaatacca ttcattttgt
    18901 cgtcaattct gtttatgatg acttttgaat acatttccct tacactagca atcagatatt
    18961 ttgcactgcc ggattggcca agagatacat tcacgatcaa gcctacacct tgcacgttgc
    19021 ccccttttat gatctgttta taggtgctgc cttcatgaat actggtgaca acttttactg
    19081 ccttggcctg tggtaagctg taaggctgat cctgtaacct ttccttggaa cagactgagc
    19141 tatctatcct tgatctgctc cattgcttcg ttggaaacaa caagccagca tcagaagtat
    19201 gttcacctat cgataacatg gaggcagaat atttctgttt tatacagcct cgatatattt
    19261 aaaatctttt ttattgcttc ctatgaatat ttcgaaaaat ccgtgttatt aaagtcattg
    19321 accgtatgtc cgttgcccac tttttttcgg aattgttgtc atttggtata agtacgggca
    19381 ctgcggttct taagtgtaaa ttaagaggtg cttcattcct ggcttcccat gccagagtgt
    19441 tgtagtaaat cgagatattt ttattatttc ccatactgca tgcacatctg cctcaaattc
    19501 taatgcactc tgcacaggaa agtttaattc ggcttgttta agaatgctgc ttgctgctgc
    19561 atctattgaa aaatctgatt aacgaaaaaa ggctaatacg actttggtaa atttatacat
    19621 gattgcccac caggcaaaca aaaatacgaa aactaaaacg agcaataggg ggcgttcaat
    19681 cctgccccat atcctacgaa tacgtttcac acatatccct cgtaacatgc cctgaatatg
    19741 gagatataat accacaataa attctacttg tgaatactcg ttacaaagaa aagcttctgg
    19801 agcacaaatt gggggaataa gcaccatttt tgcgtctgtt tatggtctta tctcaacaat
    19861 cgttccaatg ggcactaacc catcaatctc ctcaatctct tcgtctgaga cagcaataca
    19921 ccctttcgtc cagtcaacgc ctgtgtgtaa atcaccaacc cacgacaaac catttttaat
    19981 gccatggatc atgatatccc cacctggaga aacacctaac tcctttgccc tttttttgtc
    20041 tttctcgttg gggtaggaaa tgcgaaggga tagatgatat tggctgttcc tgtttcttga
    20101 atcaataatg taaactcctt ctggggtttt gctgtcacct tgccgttctt ttggcccaat
    20161 tgggtttccc ccaagagcta tcttgtaggt tttgagtatc tcgccctttg atagcagtgt
    20221 taatcgccgt tctctttttt caattagaac cttgtcagct ggttctttcc ggagcgcaaa
    20281 tattttttgt tctagcactt taatctcttt cttcagtgca gtaatctcat cctctctgga
    20341 tctaaccctt tgttggagac tctcaatttg tgttttttgc ctgtcaattg ccttatcctt
    20401 catgacaaca ttgttaatat tgaatatcat catctcacta tctcgcttgt accggctctc
    20461 cggataattc tctatcagtt tctggaaaca ttccaaagat ttctgataat ctttttgctc
    20521 atttgcagga tacgagtaaa tgatgcccat ctgaaaaagg attctgtcac cttccatggg
    20581 atgtttttca agaatctgct gatatgtatt caaagaagcc tggaagtctc cttgcctgaa
    20641 aaaatcattt gcctcctgga agattgatct ggcctgaagt tcatcggcta aacaacccca
    20701 tccgcacatg agaattgatg taattataat gcaaacaaaa agactggggg ggtgtttgtg
    20761 cgtctcactt tgctttttat ggatatctct ctctgctttc ttcacgttat cgttcaatat
    20821 cctcacagct tgtaatatgg acacagcatg caacacatta taattatatg tttaaattcc
    20881 caaaattgtc cattgttggg ttttggcagg ttgatatcct ttagaaagca atggggtgtg
    20941 agaaagtgga ggtgctttag acccgtttac ttcctctcca ccccaccaaa agtaatgtta
    21001 actgcaatgc aaaaaagatt actctacttc ttcatcgatt tctggaaagc agcatcggct
    21061 tttgcagctt tttcatctgc aattttttct ctctcttctg ccttcttcac agcctcttcg
    21121 gcccggattg cagcttcatt tgccttcata gtggctgcgt ctgctgctgc tttggcagcc
    21181 tgcgcatcca tagccgcctg atcagctttg gcacggtttt gcatttcctg ttcctgaact
    21241 ctttccaaat cacctttcgt tgcgcagccc atcatactga tggcaaaaat gatcaccatt
    21301 gtgattgcca aaagaccttt tttcattttc tgatcacctc ctttcgtttg gtttacttta
    21361 ttttatcatt gacccttaat caaaacactg attctcgtag ttatctggca aatttcctgt
    21421 tctcccattc tttatgcgca cctatagctt ttccttcttt tacaccaacc catatacaat
    21481 tggcatgcca tgcacggtta ctttaaataa acctcctgat atttaaagac aatggcacac
    21541 aaggctgtgg cgaaacctgg cattttgtag gtttttcact actcagtgac actgcgaaat
    21601 ggggtaattt atcctatcta tatgaattaa cagtataatt cagtgaaggg gataccctac
    21661 agcttctctc tttcatgaca aaaaccattg tggaccacac tgaaaacaaa gcaatcattt
    21721 cttcactggt tcaactaaga taccgtccag ttccaaatca attttcaaac gtttcattgc
    21781 atcttttgcc tcccggacat cccggaaagg gcccgccata acacggtgtc tgtcgtttat
    21841 tgagagaacc gttgccggga tttgtggacc ctggtgattt ataatggcag caagcctcat
    21901 agcctcaatc ttttccgaca cagtagcagc cagaacgtac catgcatcta ctttcagttc
    21961 tggtatttct ggtttgccgt ataacttgtc aggatgcctg atttctatag tgtccatgat
    22021 atgctctctg ttgtcctcac ggatttcgga aatgggtatg ggaattcctc gtgcttcggc
    22081 gagtacttcc ttgacttttt tccagtcaag catccgcccg gtttcttttt ccattttcct
    22141 caatttcaca tgtatctttt ccaggtctcc tgagccagaa ctttcaaaag gtgcatgggc
    22201 ttcgagatac agcacttcgc cccgctgacc tgctagatat ggttggttaa tgattgaaac
    22261 aggagtttta agtggagtat ctgcataaag ctgccttatg tcctccgggt aaagccttat
    22321 acagccattg gttgccctaa gaccaatgct ggacggtttg ttggtaccat ggatcaagta
    22381 tgttggcttg cttaaataca gtgcatattc tcccagggga ttgagggggc caggtggtat
    22441 tttagcaggt aaggggtcgc ctttttttct atgatcttct gcaatggagg caggaacgtg
    22501 ccaggtcggc tttgtaacct tgcgtgccac atgcatttca cccattgggg taggccgttc
    22561 tgtggtgcca acaccgacgg ggtatgtcga taccaccaca gcattgttct ctcccttgaa
    22621 gtgaaagagt ctcattgagg ccaaattaat cacgatgcct tttcctacaa agtctggcag
    22681 gataaaactc aggggcagca taatacgctc cccagccttg ggtacccata catctactcc
    22741 ggggtttgct tctatgagag cattaattcc cagactaaag tgccgtgcaa tatccggaag
    22801 tgtgtcgcca tttttaagct taatggctgc caatcgacca actacgctat ccccatttgc
    22861 aacaaaaaaa tcttttcgat ccagataatg ttccggataa attggaggtg gctcggcctt
    22921 tttctgcaga ccctttatta cagcacatcc atggaaggat aaaataaata gagatcctac
    22981 gatgagccaa aggcaaccgg atgagcagaa caggctatta gttcgaccca tagattgcct
    23041 gaggtattcc tttactcaac aaactaaaac catcagtcaa atgcttcaca aaaatgacat
    23101 acatagtttg tttccaccat gacagcaatg acgttaactg aaattatata tagcaaatgt
    23161 gccagcagaa acgcaagttt ggcattcgcg tttctttggg tgttagcacg aaagagaaca
    23221 aaggactgcc cgtttggcgc ttaaaaaaag tggggctgtt ctgtggcagc cccactgata
    23281 tttgctttaa ttcaacgaaa tatccttatg ctacgcttga cagtctcgct gacagattct
    23341 ttacaaactc tctctctacc tctgtggctt tgctcctcat aactctatca ccaaacatgg
    23401 ccaactttcc aaccatgctg acatcgatat tgtatgagat ctccacctcg ccaccaccaa
    23461 tatctttcaa atccatagta actttatctt tcatgcggcc aagctttgtc atgtcctctg
    23521 cttcgccttc catttccagg tgggtcggag gttcaacttt ggtaagcgtc tgtttacctt
    23581 tcatcgtaac cttgatgata ccaaccttct gtttcattgt tacttcatag gtcttgtcat
    23641 cgagctgctt aatctcttca gtgcctggca gacaagctgc aagtgcctga acatcaaaaa
    23701 gcgtatccca tagcttctga atgggcgcct taacggtaaa tttcccttct ataatcatgg
    23761 aatcctccta taactgtggc atatattgct tatgcctttt aaaaaatcat gtttttggct
    23821 tacttcccct ctgcttcttt gacagctttc tcgatgattt ctgccagaag tacataactt
    23881 ctgttgcctg gagtagtagc ctgctcgatt agcagatcta taaaagattc tccatgttct
    23941 ttaacgtgtt catcgaccca ttgtctgtgg ggtttgtctc ctgttccgaa catatgacgg
    24001 gcacagtcta tagcacttcc tgtttttgcc ttcttaccgc atacaggaca tattaccggt
    24061 ttgctttgct gttcgctcat atctctctcc ttctcttact tcttcttggc ttctttatct
    24121 ttttctgcct tttctttcag ggcattgaca atcatctcag gccgaactgg gcattccttc
    24181 atctggatac cgaaacgatg gaaaattgca ttaccaatgg ctgcagcggt cgggtttgta
    24241 aggccttcac ctgcctcctt tgcgccatat ggcccttccg gttcatacgt gtctatttca
    24301 gcaatatttg ctggaggcat tcctgttgac ctgatcatct tgtaatccac caggttcggg
    24361 ttcatcatct tgccatcctc gaaaggcata tattcgaggt agccataacc ggcagccatt
    24421 gagaaggcgc cttcaagctg tccaatcaag ccaagatggt tgatgggctg gccacagtca
    24481 tgggctgtca tactatcaat cagctttatc tttcctgtct cttcatcaat ctccacctca
    24541 acaccctgga gcatatagct gtatgcagga gaaatcatac cttttctgtg gggtgtgtaa
    24601 taacctctac ccacaatcct ctggccgtcc ttaccgcgtg ttgctctttt taccagatca
    24661 gcaaatggct ctcctctttc cgggcgggca atagcatgca cccagccttc tttgatatcg
    24721 aggtcgtaca caatgttgag gcccgactgg gcataggcaa actcaagaag ctgtttcttg
    24781 gcgtcttctg ctgccatttt tacagcatta ccggtcataa gagtctgtct cgatccccat
    24841 gccccaaggt cagcagggca atggtctgta tcaccggagt gcacctttat gtcttcaacc
    24901 tttacaccaa gtgcctcagc gcagataatt gccatagtcg tattagaacc ctgaccgata
    24961 tcctgtgcac ccacatgcag ctctacagtt ccgtcgttat tgacagtaac cactgcagag
    25021 gagaatgagt agggggtatc aaaccagttg aagattccac cggacataaa cccacacgca
    25081 gaaatgccta tggatctgtt ctttggcagt ttaccttttg acttgatcca agccttgatc
    25141 tcatcataac acaaatctat accacagctt gcgatagttg cctggccagg aacaacgtaa
    25201 ccctcgtggt gagaattctt tcgtctcatc tcgataggat cgagtcccaa atccttagca
    25261 atccactcga tctgctgttc agagcaccac atggcctgag gcgcaccgaa accacgcatc
    25321 gatgttgatg gtagcgtgtt tgtgtagacc ctgtaaccat cgtagcggta acctttccat
    25381 ctgtacggga aggagtggaa aaaaccagtg aggtaaagcg ctgttgcgcc catacctgtg
    25441 tacgcgccac cgttcgtgaa gactttggct tcacgggcac aaaacgtacc atctttcttc
    25501 acgccggttc tcgtgtaata gaacatgggg gtacgtcgtt tcgacgctat gaaatcctct
    25561 tctctggtaa aaacaatttt gacaggttta cggaccttca tagagagaac acatgcacag
    25621 aactgtgctg catcaagctc aaatttccc
//
LOCUS       DAOOMC010000025        25526 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdus sp. isolate AMR_MDS_2685
            NODE_1806_length_25526_cov_6.901025, whole genome shotgun sequence.
ACCESSION   DAOOMC010000025 DAOOMC010000000
VERSION     DAOOMC010000025.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864760
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdus sp. (wastewater metagenome)
  ORGANISM  Syntrophorhabdus sp.
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae; Syntrophorhabdus.
REFERENCE   1  (bases 1 to 25526)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 25526)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,729
            Genes (coding)                    :: 2,708
            CDSs (with protein)               :: 2,708
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 7 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25526
                     /organism="Syntrophorhabdus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1806_length_25526_cov_6.901025"
                     /isolate="AMR_MDS_2685"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2699756"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(431..3415)
                     /locus_tag="PK125_04055"
     CDS             complement(431..3415)
                     /locus_tag="PK125_04055"
                     /inference="COORDINATES: protein motif:HMM:NF014788.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIR synthase-related protein"
                     /protein_id="HPB37315.1"
                     /translation="MAHRIEIAYKAGARDVPGEKLKKRIKRDMGLDVNAKVVAVYTID
                     SDLDKDILQILRDDAFVDPVIQEGYLDKPVPMTGDWAIEVGFKPGVTDNVGRTAKEVI
                     ESLSGRRFEEEEGVYTSHIYFLSGKLTESDIIAIAEGMLANTLINRYVYKSAAQYKSN
                     GGMMVFVPRVSIGHEATVEIFPITTGLEEMMRINRERTWALSVDELKEIQKYFLKKSV
                     IDSRKKAGLTESPTDVEMEAIAQTWSEHCKHKIFNAVIEYEADGKKEVIESLFRTYIT
                     GSTDAIRRKKGRKDFCLSVFKDNAGIIRFNKRYNLAFKVETHNTPSALDPYGGALTGI
                     VGVNRDPFGTGMGAKLIFNTNVFCFAPPDYKGTIPPRLLHPKRVLEGVREGVEHGGNK
                     SGIPTVNGSLVFDENYLGKPLVFCGTCGIMPREILGKPSHEKKALKGDAIVMVGGKIG
                     KDGIHGATFSSEELSEASPTSAVQIGDPITQKRMTDFLLIARDRGLYSSITDNGAGGL
                     SSSVGEMAEDTNGCILHIDRAPLKYHGLAPWEILLSEAQERMNVSVSPEHLEEFMELS
                     EKMNVTSTILGEFTDSGKFHVLYQGKTVAYLDMDFLHDGLPKMKLKAKWERKIRDEKP
                     FEEPLSYGEALMKVLGRWNVCSKEYVVRQYDHEVQGGSVVKPLTGSQNNGPSNAGVVR
                     PDLNGDEGVVISHGICPKYSAFDTYHMMSCAIDEAIRNNIATGGSLDRMALLDNFCWS
                     DPVFSERNPEGPYKLAQLVRANKALFDCTTFFGTPCISGKDSMKNDYIFNNIKISVPQ
                     TVLISALSVINDVSRVVTMDFKEDGDIIIVVGKTYPEMGGSEYFAHNGLVGNIPPKVR
                     MTLAKRTYKTIEKAIRQNLLRSCHDASDGGLGCALAESAFAGDVGVEIDLTAAPASAI
                     FRDDFLLFSESQSRFVVSIRRKDLHTFAELFRSIPFGVLGETRKDKRFILKGLQGKTI
                     IDLPVDNLLHAWQEPFKEHFSG"
     gene            complement(3415..4179)
                     /locus_tag="PK125_04060"
     CDS             complement(3415..4179)
                     /locus_tag="PK125_04060"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HPB37316.1"
                     /translation="MHIFVNVPYRMVEKNLERASSLSIGLEIYIDNHLIEEIDTGHVK
                     ELGKKLRDFNIPCTVHAPFMDLSPGGYDRLVRRITREKLKKTIEIGNILEAKACVCHP
                     GYDKWRFDGNEQLWLEGSIETWSEVLGAANKDLLVIIENIFEETPETLIALFDYFREK
                     NLWFCFDSGHFNLFSKTPLDDWLIPLKEKIKEMHLHDNNRTFDDHLPIGSGSFPFREL
                     KAFLEQIRMKEIIFTVEPHSEATVVESIKRLKEFLS"
     gene            4530..7343
                     /gene="uvrA"
                     /locus_tag="PK125_04065"
     CDS             4530..7343
                     /gene="uvrA"
                     /locus_tag="PK125_04065"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016099277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HPB37317.1"
                     /translation="MDKIFVKGARQHNLKNIDVELPRNKMIVVTGPSGSGKSTLAFDT
                     IYAEGQRRYVESLSSYARQFLELMEKPDVDYIEGLSPAISIEQKAISKNPRSTVGTVT
                     EIYDYLRLLFARIGHVYCYGCGKEITSQHAPQIVDTILAAGAGAKLSILAPIIRGRKG
                     EYRKEIDDLRKQGFTKIKLNGTIVDLAEEIVLDKNKKHEIDVVIDRITIRDGIERRLF
                     EAVELALQKANGIVKVESEKETMVFSEMFACPDCGISYPEITPRMFSFNNPYGACPAC
                     YGLGVKEYFDPVLIIPNQDLSLREGAVLPWSEKSPVHFMPFLEAIVNHYNIDIRKPFK
                     DLPKYVQDAILHGSNGEEITFHYNRGSRRDFVKKSFPGVIRELQEDWENAPPWEKEKF
                     ERFINLIPCPTCNGTRLKKEMLWVKINNLNIDQVAQMTLSRCLQYFKDLMLTDKEKEI
                     ARTILKEIGVRLQFLIDVGLDYITLARNSATLSSGESQRIRLATQIGSGLTGVLYVLD
                     EPTIGLHQRDNQRLLATLQRLRDLDNTVIVVEHDHDAIVSSDYVIDLGPGAGENGGRL
                     VFQGTPEELCKHDESVTGMYISDRLRIEMPKVRRKPKGFIKIKGARANNLKGINVEIP
                     LGVLTCITGVSGSGKSTLIVDTLYPLLKQKLYKSKDRAGENDGITGFEAVDRVIDIDQ
                     SPIGRTPRSNPATYTGVFTYIRELFTKLPESRVRGYKEGRFSFNVKGGRCESCSGEGF
                     VKIEMQFLPDIYIVCDVCKGRRYNRDTLEVKYKGKSIADVLEMTVTQAMEFFDAFPLI
                     KNKLKVLSEVGLGYIRLGQAATTLSGGEAQRIKLSRELSKRDTGKTLYILDEPTTGLH
                     FADIDKLLHVLFELVNKGNTVVIIEHNMDVIKCADYIVDLGPEGGEKGGMVIAKGKPE
                     DLCRVSKSHTGSFLKQYLNGRTS"
     gene            7340..8077
                     /gene="kdsB"
                     /locus_tag="PK125_04070"
     CDS             7340..8077
                     /gene="kdsB"
                     /locus_tag="PK125_04070"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: protein motif:HMM:TIGR00466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HPB37318.1"
                     /translation="MKRIIVIPARYASTRLPGKPLINVGGKPLLQWVYEGAQRSKLAQ
                     SILIATDNEKVRNTALAFGAETIMTDPALPSGTDRVCAAMKGRDADIVINLQGDEPFV
                     EASIIDQLFSTMEQGNTEMATLCCPLTDERDYKDPNTVKVVLDRFNFALYFSRSSIPF
                     LKTPDSGLRTPGLSYKHIGIYAFTRQFLDLFVTMPKSPLEETESLEQLRALENGHKIK
                     VIVTQYKGFGIDTPSDLERARALLATK"
     gene            complement(8506..8976)
                     /locus_tag="PK125_04075"
     CDS             complement(8506..8976)
                     /locus_tag="PK125_04075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB37319.1"
                     /translation="MRQVCITSIMCGFFLFCPKGYAADWVYFAKDNAGNYKFYDRTSI
                     SYLSQGTVTVLKKDVYSRQGIADYVAQRAKNNLSTFGYSELSHVVCKEELNCKTREYR
                     VLACTMYDKSGSVLEKQSASNGHQAWEPAIPGSENDAFGKLICTLSPKKIKKKK"
     gene            complement(9205..9855)
                     /locus_tag="PK125_04080"
     CDS             complement(9205..9855)
                     /locus_tag="PK125_04080"
                     /inference="COORDINATES: protein motif:HMM:TIGR00730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HPB37320.1"
                     /translation="MEKQYVVDDITVKDTWRLFHIISEFVEGFENLSTIQPAISIFGS
                     ARCKEGDLLYTRAYNLSKLLGKNGFNIITGGGGGVMEAANKGAAEAGVKSVGLNIELP
                     FEQVPNRYANITLQFRYFFVRKVMFVKYAMAYIVLPGGFGTLDECFEALTLIQTKKIK
                     PFPVILVDSSYWRGLVEWMKANLLSYDKISPEDLETFKVIDEAGEIVQYLKKFVIL"
     gene            10075..10227
                     /locus_tag="PK125_04085"
     CDS             10075..10227
                     /locus_tag="PK125_04085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB37321.1"
                     /translation="MMRFTRSIQKIIRFRIHAESKIVIFIYLKNSYRYGRAVKSAQEM
                     KEIFQT"
     gene            10309..11250
                     /locus_tag="PK125_04090"
     CDS             10309..11250
                     /locus_tag="PK125_04090"
                     /inference="COORDINATES: protein motif:HMM:NF012692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HPB37322.1"
                     /translation="MRMGIDIGGTKIVAGIVDNSGKVVARKKIFVGHQKSYPVVRDAI
                     ADMVLGLLSEKTIPRESLKRIGLACAGQIDKDFKKIIFSPNLGWHNAPIRDDIEALLG
                     IDAFVENDVNAATYGEWRFGAAKGTSHVLGVFIGTGIGGGIIINGKVFRGFRNAGAEI
                     GHIILNPFGYPCNCCNTGCFEAYCGGAYIVERVKKHIKNGYRGKIWELINGDISILNA
                     GHVESGADIGDGLCKNIWNEVLEYLGAGLASMVNLLNPEIIVLGGGVVYGTHHLIEDV
                     KPILKKRSMAASLKNMRIVKAKLKEDAAIIGAAFVEP"
     gene            11292..11576
                     /locus_tag="PK125_04095"
     CDS             11292..11576
                     /locus_tag="PK125_04095"
                     /inference="COORDINATES: protein motif:HMM:TIGR04076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04076 family protein"
                     /protein_id="HPB37323.1"
                     /translation="MGEDRIGFRVIGTVRSVKGHCNAGHKVGDQVELSGHNTGNMCGF
                     FYHDLFPYVIMLQFGGGFPPEWGNPDVVEMSCMDKMNEVTIELKRIRDNE"
     assembly_gap    11667..11766
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <11767..13197
                     /locus_tag="PK125_04100"
     CDS             <11767..13197
                     /locus_tag="PK125_04100"
                     /inference="COORDINATES: protein motif:HMM:NF014877.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HPB37324.1"
                     /translation="TEWVTYFQSLGEGRCEPQKKDRTMNKVIFREYDIRGDVEQDLAD
                     PVVRDIGRAFAAYMAERGKKKASLGRDCRLSSEHFRNLVVEGMIEGGLNVVDLGLVPT
                     PLFYYSLFTLDVQGGIMITGSHNPPSMNGFKVAFGKSTIFGDEIQYLRKLIEEKRFNR
                     GKGAYSEYNDIVKDYYSFLRSNINLKKSFNIVVDAGNGTGGVVAVPILREMGQHVTEL
                     FCEMDGRFPNHFPDPTVEKNMETLRRTILEQKADIGIGYDGDSDRIGVVDDKGNIIWG
                     DYLMIIFARDILKTHRGATFVSEVKCSKNLFDDVKKHGGIPVMWKAGHSLIKQKMKET
                     GALLGGEMSGHIFFADKFFGFDDAIYSTFRILEIMGKDDRPLSDYLLDIPKTYSTPEI
                     RIDCPDTVKFDVVNKLTNYYRNKFNIIDIDGVRILLDDGWGLVRPSNTQPILVLRFEG
                     QSKEALEKIQSMVMEDLEGVMKGQLQ"
     gene            13288..15678
                     /locus_tag="PK125_04105"
     CDS             13288..15678
                     /locus_tag="PK125_04105"
                     /inference="COORDINATES: protein motif:HMM:TIGR03960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03960 family B12-binding radical SAM
                     protein"
                     /protein_id="HPB37325.1"
                     /translation="MLDIVNFITRPARYTGIEPNRIIKDPDEVSVRFALCFPDLYEVG
                     MSYYGFFLLYGIANSVQSVWCERCFAPWSDMDTYLRQNGRPLTTLESRTPLSAMDMIG
                     FSLSYELNITNILNMLDLAGIPLRAEDRKKGPIVVGGGPSMLNPAPFQKFFDIIVIGE
                     ADKKLPEILHVLKTLKGEPHEKVIGGLAQLEGVYSPLFPKTPVRRQFVEDLDDSYHPT
                     RPPIPVVGSIHDRFNVEISRGCGNGCRFCLAGFGYRPYRERSFEKLKEIIDLGMQGTG
                     YEEISLLSLSSGDYTSLFDTIAYIKERYRGVSVSLPSLKIGSIREREISLMGDIARTG
                     FTFALESATEGMRCRINKNIDVDVLLGQLPLLKQYGWRKLKLYLMVGFPWETEEDFLA
                     IKEILSPFEKERININLSVSPFIPKPHTPFQWLPMENEVILSEKISMIRKILKGRRVK
                     VKYRDPRTSLVEAIVARGDSRLWPLFEYLFHKGVKLEAWREYFQPALYDEWFNQQGIS
                     IEDYLGKRPIESALPWDIVDTGIDKSFLAHEFILAEAGKKTGDCYTGCASCGIGCTES
                     GQTRDSSFSAVRSAFVENPVQDFELSQFEIQNSKLKIDTPSIQTPAHAEVPADRPNCE
                     RIPGIKYTFRYGKYGDARYIGHLDMMNILLRAFRSLGITIRTHGKFHPMPKISLSDAL
                     PIGIESTCELIEIETDTGILIHEKMIGEINKILPKGTKILEFIKGSIQNMGKDYSYLL
                     IADKSIDMELWKLGYNSKRHFYTWKGKGIKELRAKGDFQRIIKVEDRRIHGLRADN"
     gene            15656..17140
                     /locus_tag="PK125_04110"
     CDS             15656..17140
                     /locus_tag="PK125_04110"
                     /inference="COORDINATES: protein motif:HMM:TIGR00757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HPB37326.1"
                     /translation="MASELIINVTFNETRIAFLENGVLVEFFIEKKNDNGMVGNIYKG
                     KVVRIVPGMDAAFVDIGLEKSAFLYVGDIILDRMMYEEYEDSDYPIAINERIEGVLEE
                     GQELIVQVSREPIGQKGTRVTSKITLPGRLLVLMPGTEHIGVSRRIEQEDERKKLASM
                     LKEEICPKGYGLIARTASEGKTPEELRADLNFLKRIWESIQEKSKTVRAPSILHQDLG
                     IIFRVIRDLHSHNLKKIIVDDASVYKKVGEFLKDYLPEQGCEVVCFDERDPIFEVFGI
                     EVEIGKLLQKKIWLKSGGYIVLDYTEALTVIDVNTGRYLGRKDLEDTILRTNLEAVKE
                     IAYQIRLRNIGGIIIVDFIDMERKESRETVFQTLMEALKKDRIKTFAYPISELGLVQI
                     TRKRTRHNIVNLLTEICPTCEGSGYIKSRYTVCYEVLRELRTFCRKEGGKKINVYLSP
                     EVASLLYEEEKSSIEFIEHTYSTNVNIIANPEFSVDIFQVEGVR"
     gene            17140..17748
                     /locus_tag="PK125_04115"
     CDS             17140..17748
                     /locus_tag="PK125_04115"
                     /inference="COORDINATES: protein motif:HMM:TIGR00697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1990397 - queuosine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="queuosine precursor transporter"
                     /protein_id="HPB37327.1"
                     /translation="MGLIFVCLYVACEIIANVTAGRPVSLFGIVVPSAVFVYTLTFTL
                     VDVIHEIYGKDGSRKVILGAFCANILLAIYAYLVIHLPAPAFFLDVKSYETVFGATPR
                     IVFASLTAYLISSMVDVEVYHLWKNRVKKAKWSRVLASNTVSTLADSCIFITIAFAGV
                     MPILPLIAGQYLVKMVITVLSLPLIYTTGFRGSIERYAKSRQ"
     gene            17960..18829
                     /gene="ispE"
                     /locus_tag="PK125_04120"
     CDS             17960..18829
                     /gene="ispE"
                     /locus_tag="PK125_04120"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: protein motif:HMM:TIGR00154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HPB37328.1"
                     /translation="MTHTVLSPAKVNLFLKVVSKRPDGYHNIVSIVDIISVFDVIHIE
                     EIPDDVIIIEDDKDILPKDAANTMYRAAVALKERFKINRGVRVFVEKNIPIGSGLGGP
                     SSNAATVLKELARIWKVQINEAELNDIGKGIGADVPLFLYGNACIMRGIGNKISPVEL
                     PSLWYLIIYPNISISTRRVYEGLKIVLTKKQNDIKLVAKFSGTREVSAILENDLERIG
                     ILLCPIIKTIKDRLIESGASGTLMSGSGSSVFGIFENEEGAKQASASLTNMGKIFIAR
                     SVKSGGSHGGYGC"
     gene            19090..19161
                     /locus_tag="PK125_04125"
     tRNA            19090..19161
                     /locus_tag="PK125_04125"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:19122..19124,aa:Gln,seq:ttg)
     gene            19185..20120
                     /locus_tag="PK125_04130"
     CDS             19185..20120
                     /locus_tag="PK125_04130"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016445698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HPB37329.1"
                     /translation="MNKLRVFTGSANIPLAEKICSFLKIPLGKAIVASFSDGEVQVVI
                     DESVRGMDVFIVQPTCPPVNHNLVELLVMLDALKRASAGRITVVIPYYGYARQDRKVV
                     PRTSISARLVADLMTVAGASRILAMDLHAGQIQGFFNIPVDHLYALPVHFKYFAKIKG
                     DIVVVSPDAGGVERARELGKRLNATIAIIDKRREKANVSKVMHVIGNVSKKTAILLDD
                     MIDTGGTIVQASEALVREGARSVLACCTHPVLSGRAVEKIENSPIKELIVTNTIPLSE
                     EASKVKKIKVLDVSPILGEAIRRIHKDASVSSLFI"
     gene            20256..20879
                     /locus_tag="PK125_04135"
     CDS             20256..20879
                     /locus_tag="PK125_04135"
                     /inference="COORDINATES: protein motif:HMM:TIGR00731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HPB37330.1"
                     /translation="MEEILLRAEKRTGTGKGVTRKIRKEGGIPAVLYGRGIESVPIKI
                     SAREWDALMRRVRKNVILTMELHGDAGTESRPVMIKKIQPGFLGDYVSHIDFLQVSME
                     RTIEIEIPIHLTGTAKGVINNGIVEQHLRSIMVECLPTQIPEQIEIDVTNLEIGDSIH
                     VSDISLSGVKLLEQMNVAVVTVIPPEAEEKPVTVEEVEPEKVEKKEE"
     gene            20888..21436
                     /gene="pth"
                     /locus_tag="PK125_04140"
     CDS             20888..21436
                     /gene="pth"
                     /locus_tag="PK125_04140"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: protein motif:HMM:NF013368.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HPB37331.1"
                     /translation="MFLLCGLGNKGSEYTFTRHNIGYLVIDKFSERFRIAVQKRLCGC
                     KVGESGEIILAKPNTYMNLSGGPVASLVQRKSLTSENIVIIHDDIDMNFGRIRIRWNG
                     RDGGHKGVRSVADALRSPLFYRMKIGIGRNPAIPPEEYVLSRFYTDEVEELGDVLDRA
                     VDALHTFVFEGKEKAMSMYNRS"
     gene            21510..22598
                     /gene="ychF"
                     /locus_tag="PK125_04145"
     CDS             21510..22598
                     /gene="ychF"
                     /locus_tag="PK125_04145"
                     /inference="COORDINATES: protein motif:HMM:TIGR00092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HPB37332.1"
                     /translation="MSWSCGIIGLPNSGKSTIFNALTALTVPCQSYPFCTIEPNVGIV
                     AVPDDRLARLSETLKPEKVTSTTIEFIDVAGLIKGAHKGEGLGNRFLSHIRNVDAIAH
                     VIRCFETENVPHVYAEIDPKRDVEIVETEIIVSDLEIVEERLAKLERLAKVSKEKGVK
                     EIELLARIKSTLEQGSFIAMDYFDHTDAEIVRTFNLITTKPIFYVANIDEETLEKLPI
                     SDLEEFVRSKNSRLVYICGKLEHDLINLSPHERTSYMDLYGMTEMSIESVIKVGYEIL
                     GLITFYTTVGKEMRAWTIPRNTTCVKAAGKIHTDMERGFIRAEVINADDFVRYGSEQA
                     AREHGALRVEGKEYIVKDGDILHIRFNV"
     gene            complement(22894..24387)
                     /locus_tag="PK125_04150"
     CDS             complement(22894..24387)
                     /locus_tag="PK125_04150"
                     /inference="COORDINATES: protein motif:HMM:NF003715.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HPB37333.1"
                     /translation="MIPVEYVLLIGAVLIIVSIGIARLSENLGVPALLLFLGVGMLAG
                     SEGPGGIYFDDAHLSRSVGVIALVFILFAGGLDTKWTEVKPVFRQATILATLGVLLTA
                     VTVGLFAFSVLKFSLFTGLLLGAVVSSTDAAAVFSVLRSKNVSLRGRLKPLLELESGS
                     NDPMAVLLTIGIIQIITNPETSLGSIALLFVVQMGLGIIFGLGFGKAMVFSLNRLTSY
                     YEGVYPVFTLAFAAMTYAATAAVGGSGFLAVYLMGIYAANSEFVQKKSMLRFFDGLAW
                     LSQIAMFVTLGLLVFPSRIIPIIWTGLMCSAFLMFVARPFSVFLSLLFERMGWREKTL
                     VSWVGLRGSVPIVLATFPLLAGVADAEAIFNIVFFIVLTSALFQGWSIPVVARLLRLD
                     APIQQRRQYPIEYAPMPGVDTQLVEFIIPYSSAIAGKRVVELGLPRDSLIVLVNRDEN
                     FFVPSGGTILDGGDVVLVLVNQNNLTGVRQILMEWKKPKEKDDAKLK"
     gene            complement(24447..25325)
                     /locus_tag="PK125_04155"
     CDS             complement(24447..25325)
                     /locus_tag="PK125_04155"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HPB37334.1"
                     /translation="MRGIQSILAATDFSSYSRYAAERAAMLGATLAIQRGVLLHVLEQ
                     SWLENLKQFIGSSVKAKRKIIKNASQALDELADQIREVSGFSLEPRVLTGNTLDAIVE
                     AASDFSLLVLGARGAHPVRALALGTTSQRLLGKTGQPVLVVKNKPLTPYTRVLVAVDF
                     SEGSRKALEYSQIIAPEATIIVVHVFEPVLEKVLVYSGASEDVIHESRMKARMQTEAQ
                     LRRFIRKAGTGSFHRVGVVVQGHAPFKLPEIARKRDIELIVVGKHGRWRIEEFLLGSV
                     TLHVLSQSHCDVLVVD"
BASE COUNT         7346 a         5528 c         5856 g         6696 t
ORIGIN      
        1 aaagaaaagg gctcgtcgac tcataatgag ttattgctga acgtctggct ttcataaggc
       61 ggttcttcaa gagtcacaga tcaaagaaag gatacacggt gactgtcagc aaacagcata
      121 aacgtgtctc ccgctctgag aagaagatta ttcttgatga gttctgcact acgacgagat
      181 atcaccataa gtatgtaact atcctgttaa agtgcatcaa gtgattcaga aagcggtgca
      241 gatgaagtga tggaaaacta cactaccaga agaatgaaat tatgatggtg caaagttacc
      301 aacctttgat attccttcgg caggaattaa ttatgaggca actggcacct atgaatagaa
      361 aaaaggctct atacctcgca agctcccagc cacaggttaa ggttttctat tgcaagctta
      421 tgtggactac ctacccgctg aaatgctctt tgaaaggctc ctgccatgcg tgaagaagat
      481 tgtccaccgg aagatcaatt atcgtctttc cctgcaatcc ctttaatatg aatcgtttat
      541 cctttcttgt ttctccgaga actccaaacg gaattgatcg gaagagttct gcgaaagtgt
      601 gcagatcttt tctccttatg gatacgacaa atctactctg tgattctgag aaaagtagaa
      661 aatcatccct gaaaatagca gatgctggtg cagcagtcag atctatttca acgccaacat
      721 ctccagcaaa tgcactttct gccaatgcac aacccagtcc cccatcggat gcatcatggc
      781 acgacctcag aagattttga cgtattgcct tttcaattgt cttgtacgtt cgttttgcca
      841 gggtcattct cacctttggc gggatatttc ccacgagacc attatgtgcg aaatactcag
      901 atccacccat ttcaggatat gtttttccca caacaatgat aatgtcacca tcctctttga
      961 agtccatggt cacaactcga ctgacatcat tgatgacaga taaagcagat atgagcacag
     1021 tctgggggac agatattttt atattattaa atatgtagtc gtttttcatg ctgtctttcc
     1081 ctgatataca cggtgtgccg aaaaatgtcg tacagtcgaa gagggccttg tttgcccgaa
     1141 ccagctgtgc aagtttatat ggcccttccg ggtttctctc agaaaaaaca gggtcagacc
     1201 agcagaaatt atcgagaagc gccattctgt ccagggagcc accagtagct atattgttcc
     1261 ttatcgcttc atcgatagca caggacatca tgtgatatgt gtcaaaggcg ctgtatttcg
     1321 gacatatccc gtggctgatt acgactccct catctccgtt caaatctggt cttaccacac
     1381 ctgcattact cgggccgtta ttctgtgaac cggtgagagg tttgacaaca cttccaccct
     1441 gcacctcgtg atcatactga cgcacgacgt actccttgct gcagacattc caccgtccca
     1501 aaaccttcat caaggcttca ccgtaactca aaggctcttc gaaaggcttt tcatccctga
     1561 tttttcgctc ccactttgct ttcagcttca tcttagggag tccatcatga agaaaatcca
     1621 tatcaaggta tgccactgtc ttcccctgat agagaacatg aaactttcca gagtcagtaa
     1681 actctcccaa aatggtagaa gtgacattca tcttctccga gagttccata aactcttcca
     1741 gatgttcagg gctcactgag acattcatcc tttcctgtgc ctctgacaga agtatctccc
     1801 atggggcaag gccgtggtat tttagaggag ccctatctat atggagaatg catccgtttg
     1861 tatcctctgc catttcccca acagaggacg agagtcctcc tgccccgttg tccgtgatag
     1921 agctgtaaag acctcggtcc cgcgcgataa gaagaaaatc cgtcatcctt ttctgagtta
     1981 tgggatcgcc aatctgaact gcgcttgtcg gagaagcctc tgagagttct tctgatgaaa
     2041 aggtcgcacc atggattcca tccttcccta tcttaccccc taccataact attgcatcgc
     2101 ccttcagagc ctttttttcg tggctgggct taccaagtat ctccctcggc atgatcccgc
     2161 atgtaccaca aaataccagg ggtttaccca ggtagttttc atcaaaaaca agggagccat
     2221 taaccgtagg gataccgctc ttgttgcccc catgttcaac cccctcccgc acaccttcaa
     2281 ggactctttt cgggtggagt aaacgaggtg gaatagtccc tttataatca ggtggagcaa
     2341 aacaaaatac attggtatta aaaataagct tcgctcccat acccgtgcca aaaggatccc
     2401 tgtttacacc gacgatacct gtgagtgcac ctccgtatgg atcaagggca gagggggtgt
     2461 tatgggtctc aaccttgaag gccagattat atctcttgtt gaacctgatg atgccggcgt
     2521 tatctttgaa cacagaaaga cagaagtcct ttctgccttt cttgcgccgt atagcatctg
     2581 tggaacccgt aatgtaggtt cgaaaaaggc tctcaatgac ttctttcttt ccatcggcct
     2641 cgtattcgat gactgcatta aagatcttat gcttgcagtg ttcagaccag gtctgggcaa
     2701 tcgcctccat ttccacgtca gtgggcgatt ccgtgaggcc tgccttcttg cgggaatcaa
     2761 taacgctttt ctttaggaaa tatttctgta tctccttaag ttcgtctaca gagagagccc
     2821 acgtcctttc tctgttaatc ctcatcatct cttctaggcc tgttgttatg ggaaatattt
     2881 ctactgttgc ctcgtgtccg atgctgaccc tgggcacaaa aaccatcata ccgccattgc
     2941 tcttatactg ggccgcactc ttatagacat agcgattgat aagggtattg gcgagcatgc
     3001 cttctgctat tgcaattata tcggactcag taagcttccc agataagaaa tagatatggg
     3061 aggtgtaaac accttcctct tcctcgaaac gtctccctga taaagattcg ataacctctt
     3121 ttgctgttct gcccacgtta tccgtaacac cgggcttgaa tcctacttca atggcccaat
     3181 cgcctgtcat gggaacaggc ttatccaaat atccttcctg gatgaccggg tctacaaaag
     3241 catcgtctct caggatctgg aggatgtcct tgtctaggtc tgagtctatg gtgtaaaccg
     3301 caacgacctt tgcattcaca tcaagtccca tatctctctt gattcttttt ttcaactttt
     3361 ctcccggaac atcccgggca cctgccttgt atgcgatttc tattctgtgg gccattatga
     3421 caaaaactcc ttcaaacgtt ttatgctttc aacaacagtg gcctctgaat gaggctcaac
     3481 tgtgaaaatt atctctttca ttctaatctg ttccagaaaa gctttcagtt cacgaaaagg
     3541 aaaggacccc gagcctatcg gtagatggtc atcaaaggta cgattgttgt cgtgaaggtg
     3601 catctcttta atcttttctt taaggggtat cagccaatca tcaaggggag tctttgaaaa
     3661 aagattgaaa tgaccggagt caaaacagaa ccataggttc ttttccctaa aatagtcaaa
     3721 aagggcaatt aaagtctcag gtgtctcctc aaaaatattt tctataatga cgagaagatc
     3781 tttatttgcc gcaccgagta cctctgacca cgtctcaatg cttccttcaa gccagagttg
     3841 ctcgtttccg tcaaatcgcc atttgtcata gcccgggtga caaacacatg ccttggcttc
     3901 taggatattc cctatttcta tggttttctt cagtttttcc cgtgtgattc tccgcactaa
     3961 cctgtcataa cctcccgggc ttaaatccat gaagggggcg tgcactgtac acgggatatt
     4021 aaaatctctt agctttttac ccaattcctt cacatgacca gtatcgattt cctcgatgag
     4081 atggttgtcg atatagatct caaggccaat ggaaagactc gatgcccgct ccaggttctt
     4141 ttccaccatc ctgtatggca cgttaacaaa aatgtgcatg agggtttacc gatattgtaa
     4201 atgtacagtg gataatacaa taaatcaata aaaaaacagg agaccgttaa gcttgcaaaa
     4261 ggctatataa gttttttctc agttacactt ttttcttttt tctttcgctc ctgattttcc
     4321 ttgtccacca tagcctatcg ccagttcttg cgttatttga attagggtgc tcactctgat
     4381 aaaatatatc atgttaagca gctctgactg attcggttct gagttcagag caatatattt
     4441 cattaaaaaa gtaacgcaaa tctgccgaac ccagaataat caacttacta ttcttaacag
     4501 ggcaggcaag attcaaatat gggaatataa tggataagat ttttgtcaaa ggcgcaagac
     4561 aacataacct caaaaatatt gacgttgaac ttcctaggaa taagatgatt gtcgttaccg
     4621 gaccgagtgg ctctggaaaa tcaaccctcg ccttcgatac gatatatgct gaaggacaga
     4681 gacgatacgt ggaatctctt tcgtcatacg cccggcagtt cctggagctc atggaaaagc
     4741 ctgatgttga ttatatagaa ggcctctcac ctgccatcag cattgaacaa aaggcgatca
     4801 gcaaaaaccc tcgaagcaca gtcggcactg ttacagagat ttatgattac ctgcggcttc
     4861 ttttcgctcg tatcggtcat gtttattgct atggttgcgg caaagaaata accagccagc
     4921 atgccccgca gatagttgat acaattctgg ccgcaggtgc tggcgccaaa ctttcgatcc
     4981 ttgcacccat catacgggga agaaaaggag agtaccgaaa ggagattgat gacctcagga
     5041 agcagggttt tacaaaaatc aagctgaacg gcaccatagt cgatctggcc gaggagatcg
     5101 tcctcgataa gaacaagaag cacgaaattg atgttgtcat cgacaggata accatcagag
     5161 atgggattga gcgcaggctt tttgaggcgg tggaactggc tcttcaaaag gcaaatggca
     5221 tcgtcaaggt ggaatcagaa aaggaaacca tggtgtttag cgaaatgttt gcttgccctg
     5281 actgcggaat cagctatccg gaaatcaccc caagaatgtt ttctttcaat aatccttacg
     5341 gagcatgtcc cgcatgttat ggcctcggcg taaaggaata ttttgatcct gtgttgatca
     5401 ttcccaatca ggacctatcc cttcgagagg gtgctgttct cccgtggagc gagaaaagcc
     5461 cagttcattt catgcccttt cttgaggcta ttgtaaatca ttacaatatt gatatccgaa
     5521 aacctttcaa agatctcccc aagtatgttc aagatgctat tcttcacggg tccaatggag
     5581 aggaaattac ctttcactac aacagaggct ccaggaggga cttcgtcaag aagagctttc
     5641 ccggagtaat cagagaactc caagaagact gggaaaatgc tcctccatgg gaaaaagaga
     5701 aatttgaacg ctttatcaac cttattccgt gtcccacatg caatggcacc aggctcaaga
     5761 aagagatgct ctgggtaaaa atcaacaacc tcaatattga ccaggtcgcc cagatgaccc
     5821 tctctcgttg tcttcagtac ttcaaagatc tcatgcttac tgataaagag aaagagattg
     5881 cccgaacaat actgaaggag attggcgttc gtttgcaatt tctcatcgac gttggcctcg
     5941 actatatcac ccttgcacgg aattcggcca ctctctcatc tggtgaatca cagaggataa
     6001 ggcttgcgac acagattggt tccggtctca ccggagttct ctatgttctc gacgaaccga
     6061 ccataggtct ccaccagagg gacaaccaac ggcttcttgc cacgctccag cgcttaagag
     6121 accttgacaa cactgtcatt gtcgtggagc acgaccatga tgccattgtt tcttcggact
     6181 atgtgatcga cctggggccc ggcgccgggg aaaacggtgg taggctcgtc tttcagggta
     6241 cccctgagga gttatgcaag catgatgaat cagtgaccgg catgtatatc tcggatcgtc
     6301 tcagaataga gatgccgaaa gtacgtcgga aaccgaaagg atttattaag atcaaagggg
     6361 cacgcgccaa caacctcaag ggcataaacg tagaaattcc actcggcgtt ttaacttgta
     6421 ttacaggggt ttccggttct ggaaaaagca ccctcattgt ggataccctg tatccactcc
     6481 tcaaacaaaa actgtacaaa tccaaagatc gtgccggtga aaatgatggc ataactgggt
     6541 tcgaggcagt ggatagggta atcgacatag accagtcgcc catcgggaga acaccccggt
     6601 caaatcccgc aacctataca ggtgtcttta cttacatccg tgagctcttc acaaaactcc
     6661 ccgagtcacg tgtacgagga tataaggagg gaagattcag ttttaatgtg aagggcggcc
     6721 ggtgtgaatc atgttcaggc gaaggcttcg taaaaataga gatgcaattt ttgccggata
     6781 tctatattgt gtgcgatgtt tgtaaaggaa gaaggtataa cagggacacc ctggaagtta
     6841 aatacaaggg taaaagcata gcggacgtcc ttgaaatgac agtaactcag gcgatggaat
     6901 tttttgatgc cttccccctc attaagaata aattgaaggt tctctccgag gttggcctgg
     6961 ggtatatccg acttggccag gcagccacta ccctgtccgg aggagaagcg caaagaataa
     7021 aactctccag ggaactctct aaaagggaca caggtaaaac cctttatatc cttgatgaac
     7081 ctacaacagg ccttcatttt gcagatatcg acaaactcct tcatgtgctc tttgagctgg
     7141 taaacaaagg gaataccgtt gtaatcatag agcacaatat ggatgtgata aaatgcgctg
     7201 actatattgt ggatcttggc ccagaaggcg gagaaaaagg cggaatggtg attgcaaaag
     7261 gcaaacctga agatctttgc cgggtttcaa aaagccatac tggatcgttc ctgaaacaat
     7321 acctgaacgg gcggacatca tgaaaagaat tattgttata ccggcccgct acgcttctac
     7381 ccggttaccg ggaaaaccgc tcattaatgt cggtggcaag cctcttctcc aatgggtgta
     7441 tgagggagca caaaggtcga agcttgcaca aagtatactc attgccactg ataacgaaaa
     7501 agtcaggaat actgcccttg cctttggggc tgagacgatc atgaccgacc ctgcccttcc
     7561 gagtggaaca gacagggtct gtgcagcgat gaaaggtaga gatgcggaca ttgtgataaa
     7621 ccttcagggc gatgaaccat tcgttgaggc ctctatcata gaccaactct tctcaaccat
     7681 ggagcagggg aatacagaga tggcaaccct gtgttgcccc ctaacagatg aacgtgatta
     7741 caaggaccca aatacggtta aagttgtcct agaccgcttc aactttgccc tctacttctc
     7801 ccgttcctcc atcccattcc taaaaactcc agactccggg ctccggactc caggcttatc
     7861 ctataagcac attggcatct acgcattcac ccggcagttt cttgatttat ttgtgaccat
     7921 gccaaaaagc ccactcgaag aaacagaatc ccttgaacaa ctccgggccc tggaaaacgg
     7981 gcacaaaatt aaagtaattg ttactcaata caaaggtttc gggattgata ccccaagtga
     8041 tctggaacgg gcacgggcat tactcgcaac aaaataaatc gacaggctga attgatgact
     8101 tgtttcttgt tttccttctc ttctctttcc tgatctatgg gtttgcaaaa aacgtgagag
     8161 gcctttatta ggggtttcac ttaaaccata ccatatccac gaggctgctg ggccattgac
     8221 atgccctttc agatgtgcca tggcaacggc gatgactctt ttgatgctgt tggaggagga
     8281 ccttcttcgc agcacgtgct ttccataatg atttgaattc aacgaaaaga ctgtgcaatt
     8341 gtttctgaca aaaagcatag ctatggagtt cttcggaaac cttcaggctc attgcaaaca
     8401 agtgttgcct gacaagaagg gcacaccgca attacgaact acacaaagac ttccacagga
     8461 aataggtttt tgagtactcc tttttttgta ttcgtcttta gtctctcact tcttcttctt
     8521 tatcttcttt ggggacaaag tgcaaataag tttgccaaac gcgtcgtttt ccgatcctgg
     8581 gatagcgggt tcccacgcct ggtgtccgtt agaggcgctc tgcttttcta agacgctgcc
     8641 tgatttgtca tacatggtac aggctaaaac cctgtattct cttgtcttgc agttcaattc
     8701 ttctttacac acaacgtgtg acagctcgct atacccgaag gtgctcaaat tatttttagc
     8761 ccgttgtgct acataatccg caatgccttg ccgtgaatac acatcttttt tcaatacagt
     8821 tactgtgccc tgtgacaaat aactgatgga tgtacgatcg tagaacttgt aattgcccgc
     8881 attgtcctta gcgaaatata cccaatccgc tgcatatcct ttcgggcaaa acagaaagaa
     8941 tccacacata attgatgtta tacagacctg tctcatagga ctcctctcac tacccgtata
     9001 tttctcttgt agcagttcgt gcgagtgatg tcaatcagag caactgatag gcatcacgcc
     9061 aaagaacgtc ggaaggctca taagaatggg cagacaggac aaaatacaag gataagctga
     9121 cgatgcaata taaccagcct gttcctgagt gaaaatggat agtctcagac agtttagcag
     9181 gtagaggaaa tgacgcaggt ccaattatag tatgacgaat tttttcaagt attgaacaat
     9241 ctctcctgct tcatctatga ctttgaaggt ttcaagatct tccggggata tcttgtcata
     9301 tgaaagcagg ttagctttca tccattcaac aagccctctc cagtaagatg aatcaacaag
     9361 gataactggg aacggcttga ttttctttgt ttgaataagt gtcaaagctt cgaaacactc
     9421 atcgagggtt ccaaaacctc caggaagcac gatatatgcc attgcgtact taacaaacat
     9481 caccttgcgc acaaaaaagt aacgaaattg gagtgtaatg tttgcatacc tgtttggtac
     9541 ctgctcaaaa ggaagttcaa tattgagacc tactgacttc accccagcct ctgcagcacc
     9601 tttatttgct gcttccatca cgccgccacc gccgccggta attatattga aaccgttttt
     9661 cccgagaagc tttgacagat tgtatgccct cgtgtacaag aggtctcctt ctttgcatcg
     9721 cgcgctgcca aaaatgctta ttgcaggctg tatagtcgag agattctcaa atccttcaac
     9781 aaactctgaa atgatgtgga acaggcgcca cgtgtccttc acggttatgt catcaacaac
     9841 atactgtttc tccatgggaa ccccttttta ccttgagatt ttgagtgttg acatatatta
     9901 tttagatggt acggagatgt caaacgaaaa gggtctttcc taacgctctt cgctattatt
     9961 tatcattttt gtccgtaccc tgagcctatt tcttacagac aggcaccaat gggtttagtt
    10021 ggatggtcgt atacatgggc gaagaattgt tcggtacagg ggatttctct ttacatgatg
    10081 agatttacga ggtctatcca gaaaataatt aggttcagaa tacatgctga atcgaaaata
    10141 gtcatcttca tatacctgaa gaatagctat cgctatggta gggcagttaa aagtgctcag
    10201 gagatgaaag agatattcca gacttaggtg cagacccata ggagataaag tttcacgggt
    10261 aggtgagcaa acatttttat tctgtggggt attaaccagg agccaagcat gagaatggga
    10321 attgacattg gcggaacaaa gatagtggct ggaattgtgg acaactctgg taaggttgta
    10381 gccagaaaaa aaatctttgt gggacatcaa aaaagctacc ccgtcgttcg tgatgctata
    10441 gctgacatgg tgcttggcct tctttcagaa aagactatcc ccagagagtc attaaaaaga
    10501 attggtcttg cctgtgcagg gcagatagac aaggatttca aaaaaatcat tttttctccc
    10561 aaccttggtt ggcacaatgc acccataagg gatgatattg aggcgctgtt gggcattgat
    10621 gcatttgtgg aaaatgatgt aaatgctgca acctatggag agtggcgttt tggcgcagcc
    10681 aaaggaacta gccatgttct tggagtattc atcggcacag gaatcggcgg aggaatcatc
    10741 attaatggaa aggtattcag gggtttcagg aatgcaggtg ctgaaatcgg tcatataatt
    10801 ctcaatccct ttggctatcc ctgtaactgc tgtaacactg gatgtttcga agcttactgc
    10861 ggtggtgcct atattgttga gagggttaaa aaacatataa agaacggtta cagaggaaag
    10921 atatgggaac ttatcaatgg tgacatatcg atcctgaatg ccggacacgt tgaatcggga
    10981 gctgatattg gagatggctt atgtaaaaat atatggaatg aggttcttga atacctcggt
    11041 gcaggcctcg caagcatggt aaacctgtta aaccctgaaa tcattgtgtt gggaggggga
    11101 gttgtctatg gaacacatca tctcatagaa gacgtgaagc ctatattaaa aaaaaggtct
    11161 atggcagctt ccctaaaaaa tatgagaatc gtgaaggcaa agttgaaaga agatgcagca
    11221 attatcggcg ccgcatttgt ggaaccgtaa catgggctga tggttgcgag caaataacga
    11281 aaggagcagt tatgggcgaa gacagaattg ggtttcgggt tatagggact gtcaggagtg
    11341 taaaagggca ttgtaacgca ggccataaag ttggggatca ggtcgaactg agtggccaca
    11401 acaccggcaa tatgtgcggt tttttctatc atgatctctt tccctatgtt attatgcttc
    11461 agtttggagg aggcttcccg cccgaatggg gcaatccaga tgtagtagag atgtcctgca
    11521 tggataaaat gaatgaggtc acgatcgaac tgaaaaggat acgggataac gagtaaggtt
    11581 aagaatcgga ggatggggat aaaccaaccc atccttgaac ggaggggaag gtttgtcgta
    11641 agattaaaat tgtataaatc taaaaannnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11701 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11761 nnnnnnacag aatgggttac atattttcaa agtctaggtg aaggcagatg tgagccccag
    11821 aaaaaggata gaacaatgaa taaggtcatt ttcagggaat atgacatcag gggagatgtt
    11881 gagcaggatc tggcagaccc ggttgtcaga gacatagggc gggcatttgc tgcctatatg
    11941 gcagaaagag gaaaaaagaa agcatccctg ggaagagatt gcagactcag ttcggagcat
    12001 ttcagaaatc tcgttgttga gggcatgata gaaggaggac tgaacgttgt tgatctcggg
    12061 ctggttccta cacccttgtt ctattattct ctttttaccc ttgatgtaca gggcggtatc
    12121 atgataacag ggagccacaa tcctccctcg atgaatggtt ttaaggttgc tttcggaaaa
    12181 agcacaatct ttggggacga gattcagtac ctgagaaaat tgatcgagga gaaacgtttt
    12241 aatcgcggca aaggggcgta cagtgaatac aatgatattg tgaaagatta ttacagtttt
    12301 ctgagatcca acataaatct gaaaaagagc ttcaacattg ttgtcgatgc tggaaacgga
    12361 accggtggag ttgttgcggt ccccatactc agggagatgg ggcagcacgt taccgagctc
    12421 ttctgtgaaa tggatggccg cttccccaat cattttcccg accctacagt tgagaaaaat
    12481 atggagactt tgcgaagaac aattctcgag caaaaagctg acatcggcat cggatacgat
    12541 ggtgacagcg acaggattgg tgtagtagat gacaagggca acatcatctg gggcgattat
    12601 ctcatgatta tcttcgcgcg agatatattg aaaacccaca gaggagccac cttcgtctca
    12661 gaggtaaaat gctcaaaaaa tcttttcgat gatgtgaaaa agcatggcgg cattccggtc
    12721 atgtggaagg caggccactc tcttattaaa cagaaaatga aagagacagg agccttgctg
    12781 ggtggcgaga tgagcggaca catctttttt gcagacaagt tctttggctt tgatgatgcc
    12841 atctattcga ctttcaggat tctcgaaatc atgggcaaag atgatcgccc actctccgat
    12901 tatctgttgg atataccgaa aacttactcc acccctgaga tacgcatcga ttgtcctgac
    12961 actgttaaat tcgatgtggt gaataaactg accaactatt atcgaaacaa gttcaacatt
    13021 atcgatatag acggcgtcag gattcttctt gatgatgggt ggggactcgt cagaccttca
    13081 aatacacaac cgattcttgt tcttcggttt gagggccaga gcaaagaagc acttgagaaa
    13141 atccaaagca tggtcatgga agatctcgag ggtgttatga agggacagct acagtagaca
    13201 gaagaagttg aagtaaacct tcgtccctct cagcttctta ataccggtag cttcgcacaa
    13261 ttaactgaga tatagtagac tgaataaatg ctcgatattg ttaatttcat aacaaggcct
    13321 gcccgataca caggtattga gccaaatcgg attataaaag atcctgacga ggtgtctgta
    13381 aggtttgcgc tttgcttccc tgacctctac gaggtcggca tgtcttatta tggttttttt
    13441 ctactctatg gcatcgcaaa cagcgtacaa agtgtctggt gtgagcgttg ctttgcgccg
    13501 tggagtgata tggatactta cctgaggcag aatggtcgcc cccttacaac tctcgaatcc
    13561 agaacaccac tttctgccat ggacatgatt ggtttttccc tttcctacga actcaatatc
    13621 accaatattc tcaacatgct cgaccttgca ggaatcccct tgagagctga ggacaggaag
    13681 aaaggtccta ttgtggtggg tggaggacca tccatgctca atcctgcacc atttcagaag
    13741 tttttcgaca tcatcgttat tggagaggct gataaaaaac tccctgaaat cctgcatgtg
    13801 ttgaaaaccc tgaaagggga accgcatgaa aaggttattg ggggtttggc acagctcgaa
    13861 ggggtctatt ctccactttt tccaaagacc ccggtcagaa gacaatttgt cgaggatctt
    13921 gacgattcct atcatccaac aaggcctccc ataccagtag tgggaagcat ccatgacagg
    13981 ttcaatgttg agatatcaag aggatgtggt aatggttgca ggttctgtct tgcaggtttc
    14041 ggatacaggc cttacaggga acgttccttt gaaaaactga aggagattat tgacctgggc
    14101 atgcaaggca ccggttatga agagatttcg cttctttcct tgagttctgg tgattataca
    14161 tccctcttcg ataccatagc ctacataaaa gagaggtatc gtggcgtgtc agtctcttta
    14221 ccctcattaa aaattggaag tatcagggag cgtgagatca gccttatggg tgatattgct
    14281 cgaacaggct tcacatttgc tctcgaatcg gctacagaag gtatgagatg caggattaac
    14341 aaaaatatag acgtggacgt tcttctcgga caactccccc tgcttaaaca atatggttgg
    14401 agaaaactca agttgtatct tatggtcggc tttccctggg aaactgaaga agattttttg
    14461 gctatcaagg agatactttc cccttttgaa aaagagcgca taaacatcaa tctttcagtt
    14521 tcccctttca ttccaaaacc gcacacacct tttcagtggt tacccatgga aaatgaggtg
    14581 atcctttcag agaagattag catgattagg aaaattctga aaggcaggag ggtgaaggta
    14641 aaatataggg atcccagaac aagccttgtt gaagcaattg ttgcaagggg cgacagcagg
    14701 ctatggccac ttttcgaata cctgttccac aagggtgtaa agcttgaagc atggcgggaa
    14761 tattttcagc cggcacttta cgatgaatgg ttcaatcagc aagggatctc gatagaagac
    14821 tacctgggta aaagaccaat agaaagcgcc cttccctggg atatagttga cactggaatt
    14881 gacaagtcct tccttgccca tgagtttatc cttgccgaag caggcaagaa gacaggggac
    14941 tgttacaccg gatgtgcatc gtgtggcata ggttgtacag agtcgggtca gacacgggat
    15001 tcttcgttca gtgctgtgcg ttcggcgttt gtcgagaatc cagttcaaga ttttgagctc
    15061 tcccaattcg aaattcaaaa ctcaaaactc aaaatcgata cgccctcaat ccaaactcct
    15121 gcccatgccg aggttcccgc agacagaccc aactgtgaac gaataccggg gatcaagtat
    15181 accttccggt atggcaaata tggggatgca cggtatatcg gacatcttga catgatgaac
    15241 attcttctcc gtgcgtttcg atctctggga atcacaataa ggactcatgg taaattccat
    15301 cccatgccaa aaatatctct ttcagatgcg ctaccgattg gcatagaaag cacttgcgaa
    15361 ttgatagaga ttgaaacaga cacgggaatc ctgatacatg aaaaaatgat aggtgaaata
    15421 aacaaaatat tgcccaaggg gaccaagatt cttgagttta tcaaaggaag cattcaaaat
    15481 atgggaaaag attattcata cctcctcata gccgataaaa gtatcgatat ggaattatgg
    15541 aaactggggt ataatagtaa acggcatttt tacacttgga agggtaaagg catcaaagag
    15601 ttgagggcaa aaggtgattt ccagagaatc ataaaggtgg aggacagaag gatccatggc
    15661 ctcagagctg ataattaacg ttacgtttaa tgagacgcgt atagcattcc tggaaaatgg
    15721 tgttcttgtt gagttcttca ttgagaagaa aaatgacaat ggcatggttg gcaacattta
    15781 caaaggaaaa gttgttcgaa ttgtacctgg catggatgca gcctttgttg acatcggcct
    15841 cgaaaaatca gcctttctct atgttggcga catcattctc gacaggatga tgtacgagga
    15901 gtatgaggat tctgattacc ctattgccat caacgaaagg atcgaggggg tcctcgaaga
    15961 gggccaggaa ttgatcgttc aggtttctcg cgagcctatc gggcaaaaag gcacacgggt
    16021 gacctcgaag ataactcttc ccggcagact tctggttctg atgccaggga ctgaacacat
    16081 tggagtatcc aggaggattg aacaggaaga tgaaagaaag aaacttgcct ccatgctcaa
    16141 agaagagata tgtcccaaag gttacggact catagccaga accgcttcag aaggtaaaac
    16201 acctgaagaa ttgcgggcag atctcaactt cctcaaaaga atatgggaaa gcatccagga
    16261 aaagtcaaaa actgttcggg ccccttctat cctgcatcag gacctcggaa tcattttcag
    16321 ggtgataaga gatttacatt ctcataacct gaaaaagatc attgttgatg acgcctctgt
    16381 ttataagaag gtgggagaat ttcttaaaga ctaccttccc gaacaaggct gcgaagtagt
    16441 ttgctttgat gaaagggacc ccatcttcga ggtcttcggg attgaagtgg agattggaaa
    16501 gcttttgcag aaaaagatct ggttgaagtc agggggttac attgttctag attatactga
    16561 ggccctgaca gtgatcgatg tcaataccgg gaggtatctt ggcagaaagg atctggaaga
    16621 caccattctc aggacaaacc tggaggcggt caaggaaata gcgtatcaga taaggctgcg
    16681 caacatcggc ggcattatca tagtcgattt tattgatatg gagcgtaagg aatccaggga
    16741 gacagtcttc cagaccctta tggaggcatt aaagaaagac aggataaaga cctttgccta
    16801 tccaataagc gaacttggcc ttgtgcagat cacgcgcaaa cgaacgcggc acaatattgt
    16861 gaatctcctt acagaaatat gtcctacatg cgaaggctct gggtacataa aatcacgtta
    16921 tacggtatgt tatgaagttt tacgggagct caggaccttt tgcaggaaag aaggtggaaa
    16981 aaagattaat gtgtatcttt cgccggaagt cgcaagtctt ctgtatgaag aagaaaaaag
    17041 ctccatagag tttattgaac atacctacag tacaaacgtc aatatcattg ccaacccaga
    17101 gttcagtgtc gatatttttc aagtggaagg agtacgttag tgggtcttat ctttgtttgt
    17161 ctgtatgttg cgtgcgagat tatcgctaac gttaccgctg gtaggccggt gtcacttttc
    17221 ggtattgtcg ttccatctgc tgtttttgtc tacaccctca cattcaccct tgttgacgtc
    17281 atccatgaga tctatggaaa ggacggttca agaaaggtaa tcctcggggc tttctgcgca
    17341 aatattctcc ttgccattta tgcctacctt gtcattcatc ttcctgcacc agcctttttt
    17401 ttggacgtta aatcctatga gacagtcttc ggcgcaacgc caagaatagt atttgcaagc
    17461 ctcacagcat atctgatcag ctctatggta gatgtggagg tctaccacct gtggaagaat
    17521 cgtgtaaaaa aagcaaaatg gtcaagagtt cttgccagca acacagtttc aaccctggcc
    17581 gacagttgta tctttatcac cattgcattt gcaggtgtca tgcccattct ccccctcata
    17641 gcaggccagt accttgtgaa aatggtcata actgtcctga gcctccccct aatatacaca
    17701 actggcttta gaggatcaat cgagcggtat gcaaaaagca ggcaataggc gaaagacagg
    17761 gcgagcagga aaagtggaaa gggaataaaa aatggtgaaa gatgaaaagt taaaagtaag
    17821 cattaaagtc agtacagaac caatagtata tggcttacaa cggattacag gttatgcatt
    17881 acgaattacg attaaatcgc ttcttcaggc gtcaagcgtt tttgtcacca aatattaaac
    17941 gccaaaccaa gaactgtcta tgactcacac tgtcttgtca ccagcaaagg tcaatctttt
    18001 tctcaaggtt gtgtctaaac gacctgacgg ctaccacaat attgtaagca ttgttgatat
    18061 tatttctgtg ttcgatgtca ttcacataga agaaatccct gacgatgtta ttattatcga
    18121 agatgataaa gatatcttgc ccaaggatgc agcaaacacc atgtaccgtg cagccgttgc
    18181 cctaaaagaa aggtttaaaa tcaaccgggg tgtaagggtt tttgttgaaa aaaacatacc
    18241 cattggaagc gggctcggag gtccaagtag caacgcagct actgtactca aagagttggc
    18301 cagaatatgg aaggtccaaa tcaatgaggc tgaattgaat gatattggga aaggtatcgg
    18361 tgcagatgtt cctctgttcc tgtatgggaa cgcttgcatc atgaggggaa taggcaataa
    18421 aatttcacct gttgagttac ccagcctctg gtaccttatc atttacccga acatatcaat
    18481 atcaacaaga agggtttatg aaggactaaa aatcgtgttg acaaaaaagc aaaatgatat
    18541 taaattggtt gcgaaattta gtggcactcg ggaagtttct gcaattctag aaaacgacct
    18601 cgagagaatc ggtatattgc tgtgcccgat aattaaaacg atcaaagata gattaattga
    18661 atctggggcc agcggaacac tgatgagtgg tagcggctca tctgtttttg gaatatttga
    18721 aaacgaggaa ggggcaaaac aggcatcggc ttcattaaca aatatgggga agatttttat
    18781 agcacgcagc gttaaatcgg gggggagtca tggaggttac gggtgctaag atatttctta
    18841 ttagcggtag ccaggtaaag gcttaatgtc taaattgttc ttgatgattg ttgtgttgta
    18901 aaagatataa agatgataga tgaggaaggc aagatttttg tagttatacc cataaaaaat
    18961 gaaagatggc ttgtatagag acacagtcca tccaataaag aaggccacaa ggcaaaagat
    19021 cgagatatgc gttctaaatg cctacaaaaa aggagttaaa taagcaacca attttgtaaa
    19081 acaggccatt ggggtgtcgt caagcggtaa gacacaggcc tttggagcct gcattcggag
    19141 gttcgaatcc tcccacccca ggatattcta ccaaggagcg atgcgtgaac aagttaagag
    19201 tttttactgg aagtgcgaat ataccacttg ctgagaagat ttgcagcttt ctcaagatac
    19261 ccctgggcaa ggctattgta gccagtttca gcgacggcga ggtacaggtt gtaattgatg
    19321 aaagtgtgag ggggatggat gtattcattg tacaacccac atgccctccg gtcaaccata
    19381 accttgtcga gctgctggtt atgctcgatg ccctgaaacg agcatctgca gggcgtataa
    19441 cggtcgtaat tccttactat ggttatgcgc gacaggacag gaaagtcgtt ccccgtacat
    19501 ctatttctgc aaggcttgtg gcagatctca tgactgttgc cggtgcttca agaatcctgg
    19561 ccatggatct tcatgcaggg caaattcagg gcttttttaa tattcctgtg gatcatctct
    19621 atgccctgcc ggtacatttc aagtattttg caaagattaa gggtgacatc gtagtagtat
    19681 ctccagatgc tggtggtgtt gaacgggcaa gagagttagg caaaaggttg aacgccacca
    19741 tcgcgatcat tgacaaacga agagagaaag ctaatgtatc aaaagttatg catgttattg
    19801 gtaatgtgag caaaaaaacc gccatacttc tcgacgatat gatcgacact ggaggaacta
    19861 tagtacaggc atcagaagcc ctggtaaggg aaggtgctcg atccgttctt gcttgttgta
    19921 cccatccagt gctttccgga agggctgttg aaaagattga gaattcaccc attaaagaat
    19981 tgattgtgac gaatactata ccgctgtcag aagaggcaag taaagtaaag aagatcaagg
    20041 tgcttgatgt gtccccaatc cttggtgaag caatcagaag gattcacaaa gatgcttcag
    20101 taagttcact atttatatag attccctttt ggtgaaaacc tgtatgggca tgtcctggga
    20161 gtaccatgac tgaaaaaggg agttcagcaa gttttaattg ctaacggggt ggcgtgagcc
    20221 cggtagaagt ttatgtatat aacaggaggt ctgggatgga agaaatatta ttgagggcag
    20281 aaaagagaac aggtaccggt aaaggcgtga cccggaaaat tagaaaagaa ggaggtatac
    20341 ctgctgtact ctatggcaga ggcatagagt cggttccgat aaaaatttcg gcaagagaat
    20401 gggatgcact tatgagaaga gtacggaaga acgtaatcct taccatggaa ctccacggag
    20461 atgcaggtac agaaagtaga cctgtcatga tcaaaaagat tcagcctgga tttctcggag
    20521 attatgtctc ccacatagat tttcttcaag tatccatgga aagaacgata gagatcgaaa
    20581 tacctattca ccttaccggg acagcaaaag gagtcataaa caatggcatt gtggaacaac
    20641 atttgagatc gatcatggtc gagtgtctgc ctacccaaat tccagagcag atagagattg
    20701 atgtgacaaa ccttgagatt ggagattcca tacatgttag cgacatctct ctctctggcg
    20761 tgaagctttt ggagcagatg aatgtggcag ttgtgacggt tataccccct gaagcagaag
    20821 aaaaaccagt gaccgttgaa gaagtagaac ctgaaaaggt agagaaaaag gaggaataaa
    20881 gtcctttttg tttttactat gcggccttgg aaacaaagga tctgaatata ccttcaccag
    20941 gcataacata ggttatctcg ttatagacaa gttttctgaa aggtttcgta ttgcagtaca
    21001 aaaacgcctc tgtggttgca aagttggcga atcaggggag attatcctcg ccaagccaaa
    21061 cacctatatg aacctctccg gcggacctgt tgcgtcgctg gtgcaacgta aatctttgac
    21121 ctctgagaac attgttatca ttcatgatga cattgatatg aatttcggaa ggattcggat
    21181 acgctggaat ggtagggacg gaggtcacaa aggcgtccgg tctgttgcag acgcattgcg
    21241 ctcaccgctt ttttaccgga tgaagattgg cattggcaga aatccagcca tacccccgga
    21301 agagtatgtt ctatccagat tttacacgga tgaagttgag gaacttggtg atgttctgga
    21361 ccgtgctgta gatgccctcc acacttttgt tttcgaggga aaagaaaagg caatgagtat
    21421 gtataatagg agctgacagc tcatggctcc gggctctttg tgaaaactat agactcggag
    21481 cctgaaactt ggagctcaag ggaggggata tgagttggtc atgcggaatt attggtctac
    21541 caaattcagg gaaatcgacc atctttaacg ctctaactgc acttaccgta ccatgtcagt
    21601 cctacccttt ttgtaccatt gagccgaatg tcggtattgt tgcagtacct gatgacaggc
    21661 tcgccaggct ctcagaaacc ctcaaacctg aaaaggttac atcgacaact atcgagttta
    21721 tcgatgtcgc aggcctcatc aagggtgccc ataagggtga gggtctgggc aacagatttc
    21781 tttcacatat caggaatgtg gacgctattg cccacgtcat acgatgcttt gaaacggaaa
    21841 atgttcccca cgtttatgca gaaatcgacc caaaaaggga tgtggagatt gtggaaacag
    21901 agattatagt ctctgatctg gagattgttg aagaaaggct cgcaaagctg gagaggcttg
    21961 ccaaagtctc caaggaaaaa ggggttaagg agatagagct tctcgcaaga atcaaatcca
    22021 cccttgagca gggttccttt atcgctatgg attattttga ccacacagac gcagaaatcg
    22081 ttcgtacctt caaccttatc actaccaagc ccatctttta tgttgcaaat attgacgaag
    22141 aaacactgga aaaacttcca atctctgatt tagaagagtt tgtccggtca aaaaacagca
    22201 gactagtcta tatatgcggc aagcttgagc acgatcttat aaacttgtcg ccccatgagc
    22261 gaacatcata catggacctt tacggtatga cagagatgtc tatagaaagt gtaattaaag
    22321 ttggttatga gatcctgggc ctcattacct tttatactac tgtgggcaaa gagatgaggg
    22381 catggacaat cccccggaac acaacatgcg tgaaagcagc aggcaagata cacacagata
    22441 tggaaagggg attcatcagg gctgaggtga tcaatgcgga tgactttgta cggtatggat
    22501 cagaacaggc tgcccgggaa cacggggctc tgagggtcga aggaaaagaa tacatcgtaa
    22561 aagatggaga tattctgcat attcgattca acgtatagct caactcaaaa gcgtaagcca
    22621 agtaaaaaat ttgtaccagg caggtttgat aggttgctca agtgtttcca tagtttgttg
    22681 tggtgattgt tttcacaact aaaatgcagc gcagttctct tataaattgt ctttttgctt
    22741 gcttttaaat cttatattgt tttgcttgtt caaagctctt caaggctgaa aaatgtaaag
    22801 tgaattagtg aatagggctg catctgatga gcaaatatat tttatgatgg tattgtcaat
    22861 tctcatcgtt tcaataggga ttaattttat ctgctacttc agttttgcat catctttttc
    22921 ttttggtttt ttccactcca tcaatatctg ccgcacacct gtcaggttat tctgattgac
    22981 aaggaccaaa acaacatcac caccgtcaag aatggtgcct ccactcggta caaaaaagtt
    23041 ctcgtcacgg ttcaccagta caatgagact gtccctaggc agacccagtt ccacgactcg
    23101 tttccctgcg atagccgaac tgtatggaat aatgaattca acgagttgag tgtcaacgcc
    23161 cggcatcggc gcgtactcta tgggatattg ccgcctttgt tgaatcgggg catcaagtct
    23221 caggagacgg gcgacaacag gaatcgacca accttgaaac agtgctgaag tgaggacaat
    23281 aaagaaaacg atattgaata tagcttcagc atctgccacc ccagcaagaa gagggaaagt
    23341 cgccaggact atcgggactg accctctcag gccgacccat gaaacaaggg ttttttcccg
    23401 ccagcccata cgctcaaaga gcaaagaaag aaaaacgctg aacggacgtg caacaaacat
    23461 gaggaacgct gaacacataa ggcctgtcca tatgatagga ataatacggg atggaaatac
    23521 aagcagtccc aaggtgacaa acattgctat ctggctgagc caggcaagac cgtcaaaaaa
    23581 acgcaacatg cttttctttt gtacaaattc actgttggct gcatatatac ccatcagata
    23641 cacagccagg aagccgctcc caccaactgc agcagtagcg gcatatgtca tcgctgcaaa
    23701 ggcaagggta aaaacagggt aaaccccttc gtaataggat gtcaaccgat tcaacgaaaa
    23761 aaccattgcc ttcccaaaac caagaccaaa aataatgccg agtcccatct gaacaacaaa
    23821 gagtagagca atggagccga gtgaggtctc tggattcgtt atgatttgaa ttatccctat
    23881 ggtgaggagg actgccatgg gatcattact ccccgactca agttcaagca ggggctttag
    23941 ccggccacgt aaactaacgt tctttgatcg gagcactgag aaaacagcgg ctgcatcagt
    24001 cgatgaaaca acagccccga ggagcaggcc ggtgaatagt gaaaatttta aaacagagaa
    24061 ggcaaaaaga ccaacagtca cggccgtgag aaggacgccc agcgttgcca ggattgtagc
    24121 ctgccggaag actggtttga cctctgtcca ttttgtgtca aggccacctg cgaagaggat
    24181 gaagaccaat gcaatgacgc ctactgatcg agacagatga gcatcgtcga agtaaattcc
    24241 accggggcct tctgaacctg caagcatccc tacgccgaga aacaacaaca gtgcaggtac
    24301 accgagattc tcggagagcc tggcaattcc aatgctcact atgataagga ctgcccctat
    24361 aagaagaaca tattcaaccg gtatcatgca cttactatat aatcaaccat ccattcagtc
    24421 aacaaatagg cgctgtccgt ggtctgttag tcaacgacca gcacgtcaca atgggactga
    24481 gagagaacat ggagggtaac actgcctaaa aggaattcct ctattctcca tcttccgtgt
    24541 tttcccacaa cgataagttc aatatcacgt ttacgtgcta tttcaggcag tttgaatggt
    24601 gcatgtcctt gcaccacaac tcccaccctg tgaaaagacc cggtacctgc ctttcgaatg
    24661 aaacgcctca attgtgcttc tgtctgcata cgagccttca tgcgagactc atgaataaca
    24721 tcttccgaag ctccactata aaccagtacc ttctccagta ctggttcgaa aacatggaca
    24781 acgataattg tcgcctctgg ggcaattatc tggctgtatt cgagagcttt acgtgaacct
    24841 tctgagaagt caacggcaac gaggacccgt gtatagggtg ttaatggttt attcttcaca
    24901 acaagcacag gttgtcctgt ctttccaaga agccgttgcg aggtagtacc aagagccaga
    24961 gccctgaccg ggtgcgcccc acgtgcgcca agtacaagta agctgaaatc tgatgctgcc
    25021 tcaacgattg catccagcgt attccccgtg aggacacgtg gctcaagtga aaaccctgac
    25081 acctcacgaa tctggtcagc taactcatcc agggcttgtg aagcattctt aatgattttg
    25141 cgtttagctt taaccgatga ccctatgaac tgcttcaaat tttcaagcca cgattgttcc
    25201 agcacatgca gcagcacccc tctctgtatg gcaagagtag cgccaagcat agctgcacgt
    25261 tctgctgcat aacgagaata agaagaaaaa tcagttgcag ccagaataga ttgaatacct
    25321 ctcatatttt ttctccctga aaagctgcga ttgggaatgg atttctttaa gactcatgat
    25381 tccaactaaa ccgatgatcc tttcgaattc ctttactttg tatttaaagt ttgtgaatca
    25441 gccggcaaac tggacttggt cacttatgcc acatccctct ccggctgata cacgatctta
    25501 aggaccttga gccgcttcgt tcctgc
//
LOCUS       DAOOMC010000026        24554 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdus sp. isolate AMR_MDS_2685
            NODE_1922_length_24554_cov_5.654108, whole genome shotgun sequence.
ACCESSION   DAOOMC010000026 DAOOMC010000000
VERSION     DAOOMC010000026.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864760
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdus sp. (wastewater metagenome)
  ORGANISM  Syntrophorhabdus sp.
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae; Syntrophorhabdus.
REFERENCE   1  (bases 1 to 24554)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 24554)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,729
            Genes (coding)                    :: 2,708
            CDSs (with protein)               :: 2,708
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 7 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24554
                     /organism="Syntrophorhabdus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1922_length_24554_cov_5.654108"
                     /isolate="AMR_MDS_2685"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2699756"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1291
                     /locus_tag="PK125_04160"
     CDS             <1..1291
                     /locus_tag="PK125_04160"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HPB37335.1"
                     /translation="LERSEIIKISAEDLVIAENMKDKAMSLLMPRLTAGSSYVRFSDS
                     KLADNRSYLQPLDRTSFELRLDQSASLGGKELTGLRVAKEGIEKSKKDLQSVKEEYML
                     HVTTAYYDTLRTDKLSQIAQANVIRVKKYRDAAATRLKVGEITKTVLLRAEAELSGAQ
                     SEQIRTDNNYRLARAILSRMVGIQGEFRLKEGTEELGVLSDKLNEFTIEGCHTMSLDC
                     LKEKALSERTDLQAAQIQRKIAEDHVDFTKGSYWPTLAFEGVFTKRDETRETGGLVKD
                     SAYAGLRLSFPFFEGGLRVAEVQEASAKKRQADYAYADLKKTIEVEVETAYLDHITQR
                     GLLKSLTDQYIFAKENYNSVSKQFDYGLASSLDVLDANTALLDSERQLANVMYLYQFS
                     TLRLKRATGILLSTILNPTSELPEKRRMLSNNSKEKS"
     gene            1292..2479
                     /locus_tag="PK125_04165"
     CDS             1292..2479
                     /locus_tag="PK125_04165"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HPB37336.1"
                     /translation="MGRSANIFLCSVIISSLVGAVACQKKDTKTLEEKVFNVQTQDVV
                     QKPLKPFIESIGTLNPFEEVTVSAEIEGVIRSVKVEEGSQVSKGMLLAAIDDSDYSLE
                     VKRADAVFKQAEATLENTRLEFKRKDVLYKEELVTKQQYDDVTTRLSLAEAEVERAKA
                     SLSLARLKLSKTKITSPLACVIKEKRVSAGDFVRNGTPLFIIIQPNPLKLRFTVPEKD
                     VGRLRVNQEVMLRVDGFAEAEFKGRVNIVFPNVEEKTRTLQVEALVPNNNGLLKPGLF
                     AKVILYTSGERDTIVVPVTALLYEGEKVRVFVTEGDRAKERVVKLGSKYGEFMEIIEG
                     VKTGEKVVFMGQQNLSEGAKVSIQQINPQRAESDNAGHLTSAERPPQGSGTNKSQETK
                     NEK"
     gene            2587..5730
                     /locus_tag="PK125_04170"
     CDS             2587..5730
                     /locus_tag="PK125_04170"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HPB37337.1"
                     /translation="MWLADTSVKRPVFATMLVLVLVVLGIVSYPSIGVDLFPKVDLPI
                     VNITTRLKGASSEIMDIDVTDKIEESVNTINGVKTISSTSTEGSSVVTVEFVLERDID
                     LAIQDVREKISVIRAKLPTDIDEPIVEKVDPDATPVMWFALSGMRSVRELSTYADEIL
                     KEQLQRISGVGALRMYGLRLRQARIWLDADRLHAYGVTAQDVMHALQRENIELPGGRI
                     ESDTKEYTIKVKGEFPTVQQFNDMIVGYYKGSPIRVRDIGRAEDGMEEKRSVARFNGI
                     NSVSIGIQKQSGTNTIEVVDRVKNELKNITKTLPSGMNLYIAFDQSGFIKNSINEVQH
                     HLIYGGIFAILAVLIFLRNIRTTLISALAIPASVISTFAIMNAFGFTFNNMSMLALSL
                     SIGILIDDAIIVIENIQRHIEKGMSPREASFFATSEIGLAVSATTLAIVVIFIPVAFM
                     KGIIGRFFFQFGLTVVFAVMVSWFISFTLTPMMSSIFLKPHMQTQTVTTSANIVKLLP
                     KNDRLQRFLTALSDKIEVQYLWLEGYYKKFLALALNHRLIVLFIALITFAGGLGLTKF
                     IGKEFVPSEDQSRFVIRLQMPVDYSVDEVDRMGRRVEEIIRKFPEVTSLLYSQGGGLT
                     REPNKANAMINLKPKSQRKKSQEQIKAEMRKAIRTIPGIRASVENVSMIGGGQRQTNI
                     IYSIRGRDLKNLENYSREIMSQFSKLPGIVDLDTSLETGKPEVRVFIDRDKAADLGVD
                     VASVAEAVNFLISGEVDATKFKDEAKGRRYDVRVRLSPKDRMNPDDLGRLYVRAKDGR
                     LVQVSNLAYFQEAGGSSIINRVDRQRAITLFANLEGKPLGQAKDELDSISGKTLTSDY
                     SGRYKGQADTMAESFGYLMFAMLLGIVMAYMVLAAQFESFVQPIIVLLSMPFSFIGAF
                     GALFLFNQTLNIFSFIGLILLMGLVKKNAILLVDYTNVLRERGLALREAVIQAGPVRL
                     RPILMTTLAMIFGMMPIAIGIGEGAETRAPMAMATIGGLLTSLLLTLVVVPVAYDLFE
                     EYKEKRMTKKGLRETRP"
     gene            5831..6973
                     /locus_tag="PK125_04175"
     CDS             5831..6973
                     /locus_tag="PK125_04175"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HPB37338.1"
                     /translation="MSLFFITFFLIYGGIHFYVFMKARAAFASGITINITIGAIMLFM
                     ILAPVIVRLTEKAGHEELARILAFVCYTWMGAIFIFFLTGIICDLFRLAWYTSGVLWS
                     RDFSHIVSSHRFYFFLCLVATILVVIYGIFEARQLKIEHLVVSTDKIPYEVGKIRIVQ
                     ISDVHLGLIIGEERIKSIVKAVQMVKPDILVSTGDLVDVQLDNIAGLAAFFQQIHTPY
                     GKFAVTGNHEFYTGIDRALEFTEKAGFRILREEAVDIHGIVTIAGVDDEAIGGLQAGS
                     NVSEERLCAEINNSQYAILLKHRPVPYAGSLCIYDLQLSGHTHKGQIFPFFLITRLFF
                     RQYAGFYRLDNGSFLYVNRGTGTWGPPIRFLAPPEVTVIDIVRKAD"
     gene            7066..7608
                     /locus_tag="PK125_04180"
     CDS             7066..7608
                     /locus_tag="PK125_04180"
                     /inference="COORDINATES: protein motif:HMM:NF019498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HPB37339.1"
                     /translation="MEIGLKMKELRELRKLNLKQLAELVECTPSLLSQIERGKADPSI
                     SMLKKIARALNVNIVDFFMTDFTHGDIVTRSQDRVVMQLKRWDAKIHSLVKSVVNKKM
                     QPFYTVIKPGGGSHGMYSHDGEEFGFVVKGELELMLDDKIYKVHTNESFYFSSQIPHD
                     WGNKGKEDVIVIWVITPPTF"
     gene            7686..9173
                     /locus_tag="PK125_04185"
     CDS             7686..9173
                     /locus_tag="PK125_04185"
                     /inference="COORDINATES: protein motif:HMM:NF012398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HPB37340.1"
                     /translation="MAILPEVKKHYGRLKFFINGEWIESKSNNCFENTNPATGDVIAE
                     VPVATEAEVEAAINSAWNAFKKWSNMPFRDRADYINRLRAKFDEHHEELSRILSQDHG
                     RGIDDARGTIDRCIENIESASGAMYSLYKGEHVEQLATGIDCYLMWEPVGVFLILTPG
                     NIPMHAWSSFVPYALACGCPVIVAPSWQCPVASEAMFKIADEVGFPPGVLNLLHVGTN
                     FELNSRIISDSRVAGVGFIGSTAVGRELFELCGRLGKRSSINGNGKNHIVVAPDGNLD
                     AAANYLLRGCFGMTGQRCLGSDNVAVIGNIYDDLKDKFLQAAKAMKVGFGLDETSEMG
                     PLTTLSNKKKVENFIEKGLNEGAKLILDGRELKVKGYENGYFLGPSIFEGVSPSMEIA
                     KEESFGPVSNLIRADSLEQVIEWINTNTEFGHSACLITESGKNARKFIRECNVGNVGI
                     NAGIPQPYAFFPLGSKRQSFFGVAKSRMDSVRMFLDQKTVTLRWV"
     gene            9323..10405
                     /gene="dctP"
                     /locus_tag="PK125_04190"
     CDS             9323..10405
                     /gene="dctP"
                     /locus_tag="PK125_04190"
                     /inference="COORDINATES: protein motif:HMM:NF015445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein DctP"
                     /protein_id="HPB37341.1"
                     /translation="MCDECKELSRRNFLKGALIGGAALGMGMFNGKLLHAQATNIKFS
                     TWHPPVGREVKTVWTPMLEELKKRSTGKIGYTMYAGAALGKGPEHFDIVAKGLSDMGY
                     FTATWTPGRFPLTDVLSLAVWVDGKDIAADIGNAVYNRALKDEFKNVKMLELNGCIQS
                     FIWSKKPVSKMADLKGLKLRSPGGHQTNYIKSLGAEPVFIPLGDVYMAMETGTVDGIV
                     TCPPLIQGYKLYEVAKHATVLTFGCVSEGTVMNMNSWNKLPADQKKLVEEVCTNPFRV
                     TGGLNQADYKVIMKELGDKGVKFIDLPKAEAEQWYARFQDVTRAWVKDLEAKGLPAKK
                     VVAIMNEECEKRKVHLVACPPEFKKV"
     gene            10421..10918
                     /locus_tag="PK125_04195"
     CDS             10421..10918
                     /locus_tag="PK125_04195"
                     /inference="COORDINATES: protein motif:HMM:NF016200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HPB37342.1"
                     /translation="MEQLEGFKRVIRRITYSVCAVGMFLAIPLMLITTSDVISRGFFN
                     KPIPGTLELSEYMLSIIILLGAAYTQQVKGHVGVDFLTKRFSKRTQSILEVFTTLASM
                     LIIAIMIWQGYVEGIHEKTVSDMLRVPQWPFRLLVAVGGFMLLLELFIDLFSAVARVR
                     RKITE"
     gene            10915..12225
                     /locus_tag="PK125_04200"
     CDS             10915..12225
                     /locus_tag="PK125_04200"
                     /inference="COORDINATES: protein motif:HMM:NF018513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HPB37343.1"
                     /translation="MIDPITVGILGSALVVILLLLGMPIAFLMMFVGFFGIWTLTSLE
                     AALPVVAKTVYETAANYPYTIIPLFILMGGFAGSAGITRELYETFDKWFRRLPGGLGV
                     ATIAACAGFAAVSGSSVAAAAAMGNVALPEMRRFNYHPKLATGVVAAGGTLSFLIPPS
                     LGFVVYGMLTEQSIGKLLISGIMPGVLLSLAYIVVVVGQVKMNPSLAPMTPGEVTLGD
                     KLRALKGIWETLLVFFIVMGGIYLGFINPTEAGAIGATALLVIVLIKRKLSWSGLFNS
                     LLEMARISVMVLFLVAGATVFSYFLALSTIPTVVSSWIAGLAVSKYVILTIIIAIYFV
                     LGCFLDSVSMMVLTLPVIFPVIVALNFNPIWFGVVAVLMMEAGLITPPVGLNVYTLAG
                     IAKDVPMAEIFKGATPFLLSIIVVAFILTIFPQIVMFLPNFMGG"
     gene            12292..12765
                     /locus_tag="PK125_04205"
     CDS             12292..12765
                     /locus_tag="PK125_04205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017953226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HPB37344.1"
                     /translation="MKKIDFTLNGKQTILDVEDNETLLHALREKLDIKSVKEGCSIGE
                     CGACTVLIDNEPHYSCLTLASKVSGHDVKTVEFLGDENLLHPLQEAFITYGAVQCGYC
                     TPGMLLAAYSLLLKNEHPTDTDIKEAISGNLCRCTGYIQIVEAIRNAAGLCKCNS"
     gene            12734..13570
                     /locus_tag="PK125_04210"
     CDS             12734..13570
                     /locus_tag="PK125_04210"
                     /inference="COORDINATES: protein motif:HMM:NF013134.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein subunit M"
                     /protein_id="HPB37345.1"
                     /translation="MLPDFASVIPESKEDALAYLSSLKSAKIFAGGTDLLVKMRRGES
                     YSHIVDITGIHDLKVISDHDETINIGALSTHYEISVNQIISEKAPSLAQACGWVGSPA
                     IRNMGTIGGNLVNASPAADSLAPLLIHDADVVLESKDGTRRMRLEDFILAPYKTDIQP
                     HEILTFITIKGLPGYREGYKRVTKRAAWAISRLSIAWAIQEKGGFYKDVKLAIGSCTP
                     MPFRPRKVEDSLKDRAKDEKTIQVAVGEIIDEIIKVSGMRPSFVYKIPVVKALLEDIL
                     KG"
     gene            13579..15741
                     /locus_tag="PK125_04215"
     CDS             13579..15741
                     /locus_tag="PK125_04215"
                     /inference="COORDINATES: protein motif:HMM:NF014758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HPB37346.1"
                     /translation="MVGSNRKRIDVIDKVLGKPVFAGDMKADRLLFAVVYRSPRPHAW
                     IKSIDVSGALAQPGIVKVITGEDIPGKKRFGVIKKDQPCLAEGRVRYVGEPILLIVGE
                     TENAARKAMSSVKIHFEDIEAIRDPFTAEKSGTLIHEDGNLLGHRKVIKGNVERGFAE
                     SDVIVEHTYSTTWLDHGFMETEAGVGYLDDAGRIVVVSSTQNIHYKMKEISRVLGISE
                     DMVRVIQSTTGGGFGGKLDVTVELFLALAVYYTKRPVMIRFTREESFVSNTKRHPLYI
                     EYKTGAKKDGSIQAVKVNIIGDTGPYISYGETVCLRVAVHSTGPYEVPNVHADSRMFY
                     TNNPVCGAMRGFGVPQLAFAHESQMDAVARILNMDPLDIRLKNGLRKGSSTATSQILH
                     DSVGFIDTLKKIEPFWRERKKSSHTSGFGIGCMFYGCGNTAISNPAKCHLRLTEDGKI
                     ALHSGVCEIGQGSDTILWQILLETLKIDEKNVVLVRGDTDTSSDVGSTSASRQTYISG
                     KAVYEAARKLNRYLDEKGYYRGRSLGDICEEAMTENNVIFDGYFDPPTTQLDMETSEG
                     APYATYAFATHMTEVEVDDKTGVCLVKRVHAAHDVGKAVNPRLVKGQVYGGVAMGIGL
                     ALMEEFIPGKSESLDNYYMPTSMDMPEIEVFIVEDEEPTGPFGAKGVGEPALIPQAAS
                     IINGIQDAVGVKPSQLPCHIERLKKLIEDKKSERRQEP"
     gene            15738..16889
                     /locus_tag="PK125_04220"
     CDS             15738..16889
                     /locus_tag="PK125_04220"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HPB37347.1"
                     /translation="MSVLVIKNIGTIFTGDIESPSIEGPVGIIIEDGKIKAIEKESPV
                     LADKVIDANGMTVCPGLIDSHSHPVFGDFTPRQSQLNFIESSLHGGVTSMISAGEVHL
                     PGRPTDPKGTKALALLAQKSFAKARPAGVKVYGGALILEKGLVEDDFKELAADGVWLV
                     GEIGLGSVKAAQDAKTMVEWAKKYGMKVMMHVGGTSVPGSSTVTCEDCLVANPTVACH
                     LNGGPTSVSVDEARQVIDKTECAIEVVHCGNPLTAFEIARYMKNKGILNRLIIGNDAP
                     SGTGVIPLGIWRMVNFVSSLCGIPAEIAICCATGNTKTVYGLSHGMVKVGDYADLQII
                     DAPMGSAGKDAREAIEVGDIPGIGMVLIDGIVKTQVSRNTPPPVRKVIA"
     gene            16987..18513
                     /locus_tag="PK125_04225"
     CDS             16987..18513
                     /locus_tag="PK125_04225"
                     /inference="COORDINATES: protein motif:HMM:NF012267.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HPB37348.1"
                     /translation="MKIDIERCKGCGLCEEVCPLNIITIKDKKVLVGDGCVECKTCQK
                     VCPEGVFSVIDNDDKPMCTNCPIMCRIPEGATGACLRYYNAKGEILRKGRVHTYDEVL
                     NLVGADEDSLIQKPLITGIGSGTTYPDFRPSPFIVKGVRDGIDIVTVVTEAPLSYSGM
                     KLKIDTDLYLGQESKKVYIRRKGKRHLGHLCTEEYGSKILSLGGVNILTSKDGLFAAK
                     TLFEFLQGKIIKVEVEDGPTLELALGKAPKVNGQSEEKMRVGCGSAVSGLFAPYMEKA
                     ADEVIVLDGHITGLFSEHPAGRYLKKKRSPVSIKGQKSTDGRYFLGKGTGWGGTDIQE
                     PLDVIKEIDQKKCKEGMTLLITETTGWRYAYFRMIKGEFREEQPDDGAMEFIKVLRSS
                     CEVSRVSAIFAAGVGGSARAGVTKNPIKLTKAVHEGKVSLTIGGAKPFIFPGGGINFL
                     VDVEQIKYGSVYVSPTPSFILPVEYTMSLDTFKSIGGHIDAVKPIEEVLNEVKDKATS
                     "
     gene            18522..19349
                     /locus_tag="PK125_04230"
     CDS             18522..19349
                     /locus_tag="PK125_04230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB37349.1"
                     /translation="MFIEIGPASLVVYGEKKGMPYEFDRSRLEEKIQNILRDIREYLP
                     VLKQKAYKIKNTKHMPDVPRKMVEAVKTIDEMSLTPMAAVAGSVSDALKEHIKGEGLD
                     IISVNNGGDISVFDRYEKGLKVGIGDIHTGRATSYTLDIKGFSDYGLATSGFGGRSLT
                     LGLADSVTVVAATGAVADAAATFICNNTSIITNQVRRIKASEIDPLTDIPEEYVTVEI
                     NQLSEGVINDSLEKGLQLAFKIKIKKYIYDAIIKLKDHIVTTINGGYPIILEVHNGN"
     gene            19339..19914
                     /locus_tag="PK125_04235"
     CDS             19339..19914
                     /locus_tag="PK125_04235"
                     /inference="COORDINATES: protein motif:HMM:NF018399.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid synthesis family protein"
                     /protein_id="HPB37350.1"
                     /translation="MEIRKIVTVVEEIYEEGEKKLDKPIRKVAALAVIKNPYAGKYQE
                     DLNALIDYSEELGKIISEKAVEALGKDVEVHSYGKAAIVGEKGELELAAALLHPKLGT
                     PLRAATGGGKAIIPSVKKLGSMGDSLDIPLHYKDAAFVRSHFDGMTVSISDAPKSDEI
                     VIAVAVTDGGRPHPRVGGLKKDEAKKEDGLR"
     gene            19995..20068
                     /locus_tag="PK125_04240"
     tRNA            19995..20068
                     /locus_tag="PK125_04240"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:20029..20031,aa:Arg,seq:cct)
     gene            complement(20085..20297)
                     /locus_tag="PK125_04245"
     CDS             complement(20085..20297)
                     /locus_tag="PK125_04245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB37351.1"
                     /translation="MKHETLMELGEGTVVRLLGVKNPEMVAREFHVSRQTIHDWLAGY
                     RNRDGRTLGSVSTHLAVRKGLHEDDL"
     gene            complement(20370..20906)
                     /locus_tag="PK125_04250"
     CDS             complement(20370..20906)
                     /locus_tag="PK125_04250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB37352.1"
                     /translation="MKKSLCITIFLAILLMIGAYTVQAQVNLTSFDGLWLQDSTKFRN
                     MINAGIDGSNIVPTKGGNESYKSYVCMEVEPGAPDTIKLRSYDRRGNYTDIYGNLYWL
                     SGTNSNFFGYVEFFSEDGYEYDFAYVTVINNKFTSVPGYGTYSDPTGFDTWNFLWSAK
                     IPKKVPYDIWITPCGVLD"
     gene            21637..22905
                     /locus_tag="PK125_04255"
     CDS             21637..22905
                     /locus_tag="PK125_04255"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPB37353.1"
                     /translation="MTGRNSLQYKWVIFIVLAVQYLFGYFHRVCPAVVAPELMKSFDI
                     SGMALGVLASAYFYPYAAMQIPAGILSDAWGAKKTAIMCALIAGIGSVLFGLSHTLGF
                     AIFSRILVGLGVSAMFVTSLKIFGNWFKPTEFARVSALYIAAGGVGWLTAATPLAIFS
                     QYFDWRWAFIGLGIITLLLTSLMRFTVSDRPDEIDPGTVVDTGKVQGNSMVKIGRDLQ
                     MVMKEKYFWPLAAWMFFVGAGSFGFVSLWAGPYLMDVYRFSKPAVGNILSMFPLAMVF
                     AGPFLGYVSDKVLVSRKKVIVATSIVYVACWLMMLFCHDSLPLCLLYLFFFFLGAMGT
                     GVLPIGYAATKELFTGEVAGISIGAVNLFPFLGGVVAQPLIGFILDKGGKIGTHYPSS
                     AYHSVILMYVFISILNLIAVFLTKETLRKQ"
     gene            23106..23279
                     /locus_tag="PK125_04260"
     CDS             23106..23279
                     /locus_tag="PK125_04260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010266056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HPB37354.1"
                     /translation="MAAKIDEDACTGCGTCADACPVEAITVDDVAKVNADLCTDCGTC
                     IDECPVEAISKAD"
     gene            complement(23724..>24554)
                     /locus_tag="PK125_04265"
     CDS             complement(23724..>24554)
                     /locus_tag="PK125_04265"
                     /inference="COORDINATES: protein motif:HMM:NF012449.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0030955 - potassium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HPB37355.1"
                     /translation="KSDIVLVREAAKACGRVPFIIAKIERSGALNNIDEILEAADGIM
                     IARGDLGVEIPIERIAVVQKQLMRKANMSGKPVITATQMLESMTDNSRPTRAEATDVA
                     NAILDGTDCVMLSGESAMGKFPVESTAMLVKIAVAIEPSRPGHRVREALKVIVTGNEV
                     TGGDLITLSVESALQQMPVTAVLIPSHSGGTAQRLTRLRLPIWIAAVASQDATCQALQ
                     FSYGVHPILEAENKHNWTEYAREWVKSQSLSGDFVILIEGPSPRNPRANHKLEIVDVK
                     "
BASE COUNT         6994 a         4931 c         5980 g         6649 t
ORIGIN      
        1 cctggagcgg tcagagataa ttaagatttc tgccgaggac cttgttatag ccgagaatat
       61 gaaagataaa gcaatgtctt tgttgatgcc aagactcaca gcgggaagta gctatgttcg
      121 atttagtgac agcaaactag ccgataacag gtcttatttg cagccccttg acaggacaag
      181 ttttgaatta aggcttgatc aatctgcctc tctgggcgga aaagaactga caggtttaag
      241 agtagcaaaa gaaggcatcg aaaaaagtaa gaaggattta caatctgtaa aggaagagta
      301 tatgctccat gttactactg catactatga tacactcaga acagacaaac tttcccaaat
      361 cgcacaggcg aatgtgataa gggtcaaaaa gtatcgggat gctgctgcaa cccgtttaaa
      421 agtgggtgag attacaaaaa ccgttttact gagagcggag gccgagcttt caggagctca
      481 atcagaacag atacggactg acaacaatta taggcttgcc agggcaattc tatcgagaat
      541 ggtaggtatc cagggagagt ttcggttaaa ggaaggaaca gaagaactgg gtgtgctcag
      601 tgataaattg aatgaattta ctatagaggg ttgtcataca atgagcttag actgtcttaa
      661 ggaaaaggca ctttctgaac gaaccgatct acaggctgct caaatccaga gaaagatagc
      721 agaggatcac gtagatttta caaaggggtc atactggcca accctcgcat ttgaaggtgt
      781 tttcacaaaa agagatgaaa cccgcgagac aggcgggctt gtgaaggatt cagcatatgc
      841 aggattgagg ctcagtttcc ctttttttga aggtggttta agggttgcag aagtccagga
      901 ggcaagtgct aaaaaaagac aggcggatta tgcatatgca gatttgaaga aaaccattga
      961 agtagaggta gagacagcgt acctcgatca cataacgcag cgagggcttt tgaaatctct
     1021 cacagatcag tatatctttg caaaagaaaa ttacaattca gtatcaaaac agttcgatta
     1081 tggccttgcc agcagcctcg atgttctcga tgccaatacc gccctcctgg attccgagcg
     1141 ccagcttgca aacgtcatgt acctttatca gttttctaca ttgagattga agcgagctac
     1201 aggaatattg ctgagtacta tcttgaaccc aacgagtgaa ttgccagaaa aaaggagaat
     1261 gctatccaat aactcaaagg agaaatcata aatgggaagg tctgctaaca tatttctctg
     1321 ttctgtaatt atcagcagtc ttgttggggc tgtggcatgt cagaaaaaag ataccaagac
     1381 ccttgaagaa aaagtcttca atgtacagac acaggatgtg gtacaaaaac cacttaagcc
     1441 attcattgaa tctattggta cgctcaaccc ctttgaagaa gtaacagtga gcgccgaaat
     1501 cgaaggagtt atacgaagcg tgaaagttga ggaaggatca caggtctcaa aaggaatgct
     1561 tttagcagca attgacgatt ctgattactc cctggaagta aaaagggcag atgcagtttt
     1621 caaacaggct gaggctacgt tagaaaacac aaggctggag tttaagagaa aagatgtgct
     1681 ttacaaagaa gagctagtaa caaaacaaca gtacgatgat gttacgacaa ggctctccct
     1741 tgccgaggca gaagttgaaa gggcgaaagc atccctatct ctggcaaggc ttaaactctc
     1801 aaaaacaaaa ataacttccc ctcttgcatg tgtaatcaaa gaaaaacggg tttctgcagg
     1861 tgattttgtt agaaatggaa cccccttgtt tataattata cagccgaacc ccctcaagct
     1921 tcgattcaca gttccagaaa aggatgttgg aagactaagg gtaaatcaag aagttatgct
     1981 gagggtagat ggttttgcag aggctgaatt caagggcaga gtgaacatag tttttccaaa
     2041 tgttgaagag aagacaagaa cgcttcaggt ggaagccctt gttccaaata ataatggtct
     2101 tttaaagcct ggcctttttg cgaaagtcat tctgtatact tccggtgagc gcgacacaat
     2161 tgttgttcct gttacagcct tgctttacga aggtgaaaag gtaagggtgt ttgtaacgga
     2221 aggggatcgc gctaaagaac gtgtggtgaa attgggaagc aaatacggtg agttcatgga
     2281 gattatcgaa ggagttaaaa caggtgagaa ggttgttttc atgggccagc agaacctttc
     2341 cgaaggggca aaggtaagca tacagcagat taatcctcag agggctgaat ctgacaatgc
     2401 aggccatctg acatccgcag aaaggcctcc acagggttca gggacaaaca aatctcaaga
     2461 aaccaagaat gagaaataat aaatgaactc tatccataat tacttacaag attcaagatg
     2521 gcgttgtctt tgcgttgttc gccgaaccca gagtcctaaa cccttggcta tccccgggag
     2581 tttttcatgt ggttagcaga tacttctgta aaaagacctg tttttgccac gatgcttgtt
     2641 cttgtgctcg tggtgctcgg gatagttagc tatccgagta ttggggttga tctattccca
     2701 aaagttgacc tcccaattgt aaatatcact acgcgattga agggcgccag ctctgagatt
     2761 atggatatcg acgttacgga taagattgaa gagtctgtaa atacaatcaa tggtgtaaaa
     2821 actataagtt caacgagcac tgaaggttca tccgtagtta ctgtcgaatt tgtattagaa
     2881 cgggacattg accttgcaat ccaggatgtt cgggagaaaa tctcggttat cagagcgaaa
     2941 ctccccacgg atatcgatga acccatagtg gagaaagtgg acccagatgc cacacctgtg
     3001 atgtggtttg ccctttcagg aatgcgatct gtcagggaac tttcaaccta tgctgacgag
     3061 atacttaaag aacaactcca aagaattagc ggagttggtg cattacgtat gtacggtctt
     3121 cgattacggc aggcaaggat ctggcttgat gctgataggc tccatgcata tggtgttaca
     3181 gcccaggatg tcatgcacgc cctccagaga gagaacatcg aattaccggg tggtcgcata
     3241 gaaagcgaca ccaaggagta cactatcaaa gtcaagggag aatttccaac tgtacagcag
     3301 ttcaatgata tgattgtggg ttactataag ggctcaccga tcagagtgag ggatattggc
     3361 cgcgcagaag atggcatgga ggagaagcgc tctgttgcac gatttaacgg cattaactcg
     3421 gtaagtatag gaatacaaaa acagtccggc accaatacta ttgaagttgt tgacagagta
     3481 aaaaatgaac tgaaaaacat aacaaaaact ttaccgtctg gtatgaatct ctatattgct
     3541 tttgatcagt cagggtttat caaaaattcg attaatgagg ttcagcatca tcttatatac
     3601 ggcgggatct ttgccatact tgctgtcctc atattcctgc gaaacattag aacaaccttg
     3661 ataagtgcat tggccattcc tgcctcggtt atctcaacct ttgcgattat gaatgccttt
     3721 ggttttacat tcaacaatat gtctatgctt gctctttctc tttccatagg tattcttatt
     3781 gatgatgcca taattgttat tgagaatatt cagaggcata tcgaaaaagg tatgagtccg
     3841 cgggaggcat ctttttttgc aacctcagaa ataggccttg cggtttctgc aactaccctg
     3901 gcaattgttg ttatctttat acctgttgca tttatgaaag gaattattgg acgattcttt
     3961 ttccagttcg gactcactgt tgtgtttgcg gtgatggttt cctggttcat ctccttcacc
     4021 cttaccccta tgatgtcatc catatttctt aagccccata tgcagactca gactgtaaca
     4081 acttcggcaa acatagtgaa gttgttaccg aaaaatgacc ggttgcagcg atttcttaca
     4141 gcgctatcag ataaaattga ggtgcaatat ctttggctgg agggctatta caaaaagttt
     4201 ctggcccttg cccttaatca cagattaata gtcttgttta ttgcgttgat cacatttgca
     4261 ggtgggctgg gtcttaccaa atttattggt aaggaattcg taccctctga ggatcagagc
     4321 cgattcgtta ttcgattaca gatgcctgta gattattctg ttgacgaagt ggatcgtatg
     4381 ggaaggcgag tggaagaaat aataaggaaa tttccagagg ttacctctct tttatactcc
     4441 caaggcggtg ggttgacaag ggaacccaac aaggccaacg ccatgatcaa cctcaagcct
     4501 aaatcacagc gaaaaaaaag ccaggaacag atcaaggcag aaatgcgaaa ggcgataagg
     4561 acgattcccg gtatacgagc gtcagttgaa aatgtatcaa tgattggagg gggccagagg
     4621 cagaccaata ttatctatag tatccgtggg agggacctca aaaaccttga gaattattcc
     4681 cgtgaaatta tgagtcaatt ttcaaagctc cccggtattg ttgatcttga cacttcactt
     4741 gaaacaggaa aaccagaagt acgcgtcttt atagaccgtg acaaagctgc tgatctgggt
     4801 gttgatgttg cgagcgttgc tgaagcggtc aactttctta taagtggtga ggtcgacgct
     4861 accaagttta aggatgaggc gaaaggccgc cgctatgatg ttcgtgtaag gcttagccct
     4921 aaggaccgga tgaaccctga tgaccttggt aggctctatg tcagggcaaa agatgggagg
     4981 cttgttcagg tttcaaacct agcctatttc caggaggcag gcggatcaag cattatcaac
     5041 agagttgata gacagagagc tataacactt tttgctaatc ttgagggtaa accactcggg
     5101 caagcaaaag acgagttgga tagcatttca ggaaaaactc tgacctctga ttattccggg
     5161 cggtataagg gacaggctga taccatggca gaatcttttg gttatctcat gtttgctatg
     5221 cttctgggaa ttgtaatggc atatatggtc ctcgctgcac aattcgagag ttttgtgcaa
     5281 ccaatcatcg tattactttc aatgcccttt tcctttatag gggcctttgg ggcacttttc
     5341 ctgtttaatc agaccttgaa tatattcagc tttatcggcc ttatattgct tatgggcctt
     5401 gtcaagaaga atgccatcct gcttgttgac tatacgaatg tgctgcggga gagaggtttg
     5461 gcattgcgag aagcagtaat tcaagcgggc cctgttaggc ttcgaccgat cctcatgaca
     5521 acgttagcga tgatttttgg tatgatgccc atagcaatcg ggataggaga gggggcggag
     5581 acccgcgcgc ctatggccat ggcaacaatt ggcggtctgt taacatcatt attgctgact
     5641 ctggttgttg tccctgttgc atacgacctc tttgaagaat acaaggaaaa aagaatgaca
     5701 aagaaaggct tgagagagac caggccatga gcaatcctgg tgtattgcaa aacgatacag
     5761 gcgagggagc gaccagagat ttctttaaaa gcgcaggctt cgataactcg gtgaggcaga
     5821 tggaaaacct atgagcctat tttttataac tttttttctc atatatggag ggattcactt
     5881 ctatgttttt atgaaagcca gggcagcctt tgcctccggg ataacaataa atataacaat
     5941 aggcgcgatt atgcttttca tgattcttgc cccggtgata gtccggttga ctgaaaaggc
     6001 aggccatgag gaacttgcca ggatactggc gttcgtttgc tacacatgga tgggtgccat
     6061 atttatattt tttttgacag gaatcatatg cgatttattt cggctagctt ggtatacatc
     6121 aggggtattg tggtcaagag atttttctca tattgtatcc tcacatagat tctatttttt
     6181 cttatgtctg gtcgcaacaa tcttagttgt aatatatggc atatttgaag cacgacagtt
     6241 aaaaattgaa catcttgtcg tatccacgga taaaatacca tatgaggttg gtaaaataag
     6301 gattgttcag atctctgatg ttcatcttgg cctcataatt ggtgaagaaa gaatcaaaag
     6361 cattgtcaaa gcagttcaaa tggtaaagcc tgatatcctt gtttcaacag gagaccttgt
     6421 ggatgtacaa cttgacaata ttgctggtct ggcggccttt tttcaacaaa ttcatacacc
     6481 atatggaaaa tttgcagtaa cagggaatca tgaattttat actggaattg accgggcact
     6541 cgaatttaca gaaaaggcag gtttcagaat tctcagggaa gaggctgtag atatccacgg
     6601 tattgtcacc atagccgggg ttgatgatga agccataggg ggtttgcagg caggtagcaa
     6661 tgtatcagaa gagagattat gtgcagagat taataactca caatacgcaa tactcctgaa
     6721 gcacaggccg gtgccgtacg ctggatcgtt gtgtatttat gatcttcagc tatcaggcca
     6781 tacccataaa ggtcagatat tccccttttt tctcatcacc cggttgttct tcaggcaata
     6841 tgcaggattc tatagattgg acaatggttc ttttctatat gtgaatagag gtacaggaac
     6901 gtggggacct cccatacgat ttcttgcacc tccggaggtc actgtaatcg acattgttcg
     6961 caaggccgat taactcaaaa aaatatcgct ccacaacatt tttttcttga catcatatta
     7021 aaccatattt aatctgttaa ttaatattta acgttaagga tgcctatgga aattggatta
     7081 aagatgaagg agttaagaga gctaagaaag ctgaatctca agcagcttgc tgaacttgtt
     7141 gaatgcactc catcattgct ttctcagatt gaaaggggta aagccgaccc ctccatatcc
     7201 atgttaaaaa aaattgccag ggcattgaat gtcaacatcg tcgatttttt tatgacagac
     7261 tttacgcacg gagacattgt aacaaggtca caggaccgtg ttgtgatgca gctaaaacgt
     7321 tgggatgcaa aaattcattc cctcgtgaaa agcgtggtta acaagaaaat gcaacccttc
     7381 tacaccgtta tcaaaccggg tggtggctca catggcatgt actctcatga tggtgaggag
     7441 ttcggatttg ttgttaaagg tgagttagag cttatgttag atgacaagat ttataaggtc
     7501 cacacaaatg aaagttttta tttttcatcg cagattcccc atgattgggg aaataaaggc
     7561 aaagaagatg taatcgtaat ctgggtaata acaccgccga ccttctgaat aaggtcgcat
     7621 ggttcgagat tcggagttgt gggttcaact cagagtaaca caatattaaa aggatggagg
     7681 aagatatggc tattctgcct gaggtaaaaa agcattacgg acggttaaag ttttttataa
     7741 atggtgaatg gattgagtcc aagtcaaata attgtttcga aaataccaat ccggccactg
     7801 gagatgtcat tgcagaggtt cctgtagcca cagaggcaga agttgaggca gccataaatt
     7861 cagcatggaa cgccttcaaa aaatggagta acatgccctt cagagacagg gcagattata
     7921 taaacagact gagagccaaa tttgatgaac accatgaaga attgtcccgt atcttgtccc
     7981 aggatcatgg aagaggtata gatgatgctc gtggaacaat cgacagatgc atcgaaaata
     8041 ttgaatctgc atcaggagcg atgtacagcc tttacaaagg ggaacatgtg gaacaacttg
     8101 ctacaggtat tgactgttat ttgatgtggg aaccggtcgg cgtttttctg atcctcacgc
     8161 cgggcaatat tccaatgcat gcgtggtcct cttttgtccc gtatgcttta gcttgtggtt
     8221 gtccagttat tgttgccccc agctggcaat gtcctgtggc atcagaggca atgtttaaga
     8281 tagctgatga agtgggcttt ccgccagggg tgttgaacct tcttcacgta ggaacgaatt
     8341 ttgaacttaa ttccaggata atatctgact caagagttgc cggtgttgga tttattggtt
     8401 ccactgctgt tgggagagag ctctttgagc tttgcggtag acttgggaag aggtcatcaa
     8461 taaacggaaa cgggaagaat catattgtag ttgctcctga tggtaacctt gatgctgcag
     8521 ccaattatct cctcagaggc tgcttcggca tgaccgggca acggtgtctc ggttcggata
     8581 acgtggctgt tataggcaac atctacgacg atctcaaaga taaattttta caggctgcca
     8641 aagcaatgaa ggtaggtttc ggattggacg aaacaagcga gatgggacca ctcacaacgc
     8701 tttccaataa gaaaaaggtc gagaatttca ttgagaaggg tctcaatgaa ggcgcaaaat
     8761 tgattctgga cggtcgtgag ctcaaagtca agggttatga aaatggatat ttccttggcc
     8821 cttctatttt tgaaggtgta tccccatcta tggaaatagc gaaagaagaa tcctttggac
     8881 cggtttccaa tctcatccgg gccgattctc ttgagcaggt aattgaatgg atcaatacta
     8941 atacggaatt tggtcactct gcatgtctca tcacagagag cgggaagaat gcccgaaagt
     9001 ttatacgtga atgcaatgtg ggaaacgtgg gaatcaatgc cggaatacca cagccttacg
     9061 cattctttcc tcttgggtct aagcgacaat cattttttgg tgtagcaaag tcaaggatgg
     9121 attctgtaag gatgtttctg gatcaaaaga ctgtgacttt aagatgggtt taaaaaagag
     9181 tagggtttag gggtaattat ataggtgaca tcgtttttta agtttggatg tcatatctag
     9241 gggtagaatt gatggttgag tttaggcaga cagagaggta ttattaaaga ttagttttaa
     9301 taaaatttaa aaggaggaaa aaatgtgtga cgaatgcaag gagctttcaa gaaggaattt
     9361 cttaaaagga gcactcatcg gtggtgcagc ccttggaatg ggtatgttca atggcaaact
     9421 tctccatgca caggcaacca atataaaatt cagtacatgg catccacctg tggggagaga
     9481 ggtaaagaca gtatggaccc ctatgcttga ggaattgaaa aagagaagta cgggcaagat
     9541 tggctataca atgtatgctg gtgctgcact ggggaaaggg ccggaacact ttgacattgt
     9601 tgccaaggga ctgtcagata tgggttattt cacggctaca tggacaccag ggcgttttcc
     9661 gcttacagac gtgctttctc tggccgtatg ggttgatggt aaggatattg ctgcagacat
     9721 cggaaatgca gtctataacc gtgccttaaa ggatgagttt aaaaatgtca agatgctgga
     9781 attgaatggc tgtattcaat ccttcatctg gtccaagaaa ccagtctcta aaatggcaga
     9841 tctcaaaggt ttgaaactaa gatcacctgg tggacatcag acaaactata taaaatctct
     9901 cggtgcagag ccggtcttca taccattagg agacgtttac atggcaatgg agacgggaac
     9961 ggtggacggt attgtgacat gtcctccttt gatccagggc tacaaactct atgaagttgc
    10021 aaaacatgca acggttctaa cctttggttg cgtatctgag ggcacagtca tgaacatgaa
    10081 ctcgtggaac aaacttccgg cggaccagaa gaagcttgta gaagaggtat gtacaaaccc
    10141 attcagagtg actggcggcc tcaaccaggc tgattataaa gtcatcatga aggaattggg
    10201 tgataaaggc gttaagttca tcgacctccc gaaagccgag gcagagcagt ggtacgcaag
    10261 gttccaggat gttaccaggg cctgggtgaa agaccttgaa gcaaaggggc ttccagcgaa
    10321 aaaggttgtg gcaattatga atgaagaatg tgagaagaga aaagtccatc tcgttgcatg
    10381 cccgccggaa tttaaaaaag tctgacagta aaggtttgac atggaacagt tagagggttt
    10441 taagagggta ataagacgca tcacctattc agtgtgtgca gttggcatgt tcctggccat
    10501 accgctcatg cttataacaa cgtctgatgt tataagccgt ggtttcttta acaaaccgat
    10561 accgggtacc ctggaactct cggaatatat gttgtcaatt ataatcctgc tgggtgctgc
    10621 atacacccag caggttaaag gtcacgttgg tgtcgatttt ctgactaaga gattctccaa
    10681 aaggacacaa agcatccttg aggtcttcac tacccttgca agtatgttga ttatagcgat
    10741 catgatctgg caggggtacg ttgaaggtat tcatgaaaag accgtgtcgg atatgctcag
    10801 agtaccccag tggcccttca ggcttcttgt tgcagtgggt ggatttatgc tcttgttaga
    10861 gctttttatt gatcttttca gcgcagtagc aagagtgagg aggaagataa ctgaatgatt
    10921 gatcctatta ctgtaggtat ccttgggagt gccctggttg ttattttact tcttctggga
    10981 atgcccattg catttctcat gatgtttgtt gggttttttg gaatatggac cctgacttct
    11041 ttagaggctg ccctcccggt ggtggcaaaa acggtctatg aaactgctgc aaattacccg
    11101 tacacaatta ttccgctatt tatactgatg ggaggatttg ctggcagcgc tggaatcacc
    11161 agagagctct atgaaacctt tgacaaatgg ttcaggcgtc tccctggcgg gctgggtgtt
    11221 gccaccatag ctgcttgtgc aggttttgca gcagttagcg gttcctctgt cgccgctgca
    11281 gcagcgatgg gaaatgtcgc gctcccagag atgagacgat ttaactacca tcccaaactg
    11341 gctactggcg ttgtggctgc cggcggaact ctcagtttcc ttattccgcc aagcctcggt
    11401 tttgttgttt acggaatgct cactgaacag tcaattggca agcttctgat ttccggtatc
    11461 atgccagggg tactgctttc ccttgcttat atagtagttg tcgtagggca ggtaaaaatg
    11521 aatccttctc ttgcacccat gacgcccggg gaagtgacgt taggagataa actaagggcg
    11581 ctcaaaggga tttgggaaac actccttgtg tttttcattg ttatgggtgg gatttacctt
    11641 ggttttatca atcctactga agcaggagcc ataggtgcta cagctttgtt ggtgattgta
    11701 ttgataaagc ggaaacttag ttggtcaggt ctttttaact cactcctgga aatggcaaga
    11761 atttctgtta tggtgctctt cctcgtggct ggtgccactg ttttcagcta ttttctggcc
    11821 ctttctacga taccgacagt ggtttcttca tggattgccg gtcttgccgt ctcaaaatac
    11881 gtaatcttga caattattat tgctatatat tttgtgctgg gctgttttct tgattctgtc
    11941 tctatgatgg tgctgacatt gccggtcatc tttcctgtta tcgtggcttt gaacttcaat
    12001 cctatctggt tcggcgttgt ggctgttctc atgatggaag caggcctcat taccccaccg
    12061 gtaggtctta atgtgtatac acttgcaggt atcgcaaaag atgttcccat ggcagagata
    12121 tttaaaggtg cgacaccgtt tctgctttcg atcatcgttg tggcgtttat ccttactata
    12181 tttcctcaga ttgttatgtt ccttcccaac tttatgggag gctaatgaaa tgacagcttt
    12241 taaataataa cttttagtgt tgagttaata acgattcaat taatttaaaa tatgaagaaa
    12301 atagacttta cattaaatgg taaacaaaca atccttgacg tggaggataa cgagacgcta
    12361 cttcacgctc ttagagaaaa gctcgatata aaaagtgtca aagagggctg ctccattggg
    12421 gagtgcgggg catgcacagt tcttattgac aacgaacccc actattcttg ccttactctg
    12481 gcttctaagg tttcaggcca tgatgtaaaa accgttgagt ttcttggaga tgagaatctg
    12541 cttcatcctc tgcaggaggc atttataaca tatggggctg ttcagtgcgg gtactgcaca
    12601 cctggaatgt tgcttgcagc ttacagtctc ctcttgaaaa acgagcaccc cactgacacc
    12661 gatattaaag aagctatcag cggcaacctg tgtaggtgca ccggatatat ccagattgtg
    12721 gaggcaattc gaaatgctgc cggactttgc aagtgtaatt cctgagtcaa aagaagatgc
    12781 tttagcttat ttgtcgtctc ttaaaagtgc aaaaattttt gcagggggta ctgaccttct
    12841 cgtcaagatg agaaggggcg agtcgtacag tcatattgtc gacatcaccg gaattcacga
    12901 tttaaaggtt atctctgatc atgatgaaac gatcaatatt ggtgctcttt caacacacta
    12961 tgagatcagc gtgaatcaaa tcatcagtga gaaggcgccg agccttgctc aggcatgtgg
    13021 atgggtagga tctccggcta tcagaaatat gggcactatt ggcggaaatc ttgtaaatgc
    13081 atctcctgca gcagattccc ttgcacctct tttgattcat gacgctgatg ttgtcctcga
    13141 atcaaaagat ggaaccagaa ggatgaggct cgaagatttt attcttgcac cctacaaaac
    13201 agatattcaa ccccatgaga tcctcacctt tataacgata aagggcttgc caggatacag
    13261 ggagggttac aaacgtgtaa ccaaacgggc agcgtgggca atctccagat tatctattgc
    13321 ctgggccatc caggaaaaag gtggatttta taaagatgta aagctggcca tcggctcgtg
    13381 tactcccatg ccctttaggc cgagaaaagt tgaggattca ctaaaggaca gggcaaagga
    13441 tgaaaaaacc atacaggttg cagtaggtga aatcattgat gaaataataa aggtctctgg
    13501 tatgcgacct tcattcgttt acaagattcc cgttgtgaag gctctcttag aggatatatt
    13561 aaaaggataa agggtattat ggtcggttct aacaggaagc gtattgacgt aattgacaag
    13621 gttttgggga agcctgtttt tgcaggagac atgaaggcag accgattgct ctttgctgtt
    13681 gtgtatagga gcccaagacc ccatgcctgg ataaaaagta ttgatgtgag cggagcgctt
    13741 gcccagccag gtattgtaaa ggtcataaca ggtgaggata tacccggcaa aaagcgattc
    13801 ggcgtcatca agaaggatca accctgtctg gcagagggca gggtcagata cgtcggggaa
    13861 ccaattctcc tgattgttgg tgagactgaa aatgcagcac gaaaggccat gagttctgtg
    13921 aagattcact tcgaggacat tgaagcgatc cgtgatccct ttacagcaga aaagtcaggt
    13981 acgcttatcc atgaagacgg aaacctgctc ggccatcgta aagttatcaa aggcaatgta
    14041 gagaggggat ttgcagaatc tgatgtgatt gttgagcata cttacagtac gacatggctt
    14101 gatcatggtt ttatggagac cgaagcaggc gttggttacc tcgacgatgc cgggcggatt
    14161 gttgtcgttt cctcaaccca gaatatccac tacaagatga aagagatttc acgggtcctg
    14221 gggatatccg aagacatggt cagagttatc caatccacca ccggaggcgg ttttggtggc
    14281 aagctcgatg tcactgtcga gcttttcctt gcattagcag tgtattacac aaaaagaccg
    14341 gttatgatac gctttacccg tgaggagagt tttgtctcga acacaaaaag gcatcctctc
    14401 tacatcgagt acaagaccgg agctaaaaag gacggatcga tccaggctgt caaggtgaac
    14461 atcatcggtg atacaggtcc ctatatctcc tatggcgaaa cagtctgtct ccgtgttgca
    14521 gtgcattcaa caggccccta tgaagttcca aatgttcacg ctgacagccg gatgttttac
    14581 acaaataatc ctgtatgtgg tgccatgaga ggcttcggag tgccacagct tgcctttgcc
    14641 catgaatcac aaatggatgc ggtagctcgt atcctcaata tggatcccct tgatatccgg
    14701 ctgaagaatg ggcttaggaa gggatcgagt acggcaacct cccagattct tcatgacagc
    14761 gttggtttca tcgacacact caaaaaaata gagcctttct ggagagagag aaaaaaatcg
    14821 agtcatacaa gcggattcgg aattggctgt atgttttacg gatgcggcaa tacagctatt
    14881 tcaaatcctg ctaaatgcca tctcaggctc actgaagacg gaaagatcgc cctccactcg
    14941 ggtgtgtgcg agattggcca gggatcagac acgatactat ggcagattct ccttgaaacg
    15001 ctaaagattg atgaaaaaaa tgttgtcctt gtgcgcggcg ataccgatac atcttcagac
    15061 gttggttcta cttcggcaag ccgccagacc tacatatccg gtaaagcggt gtatgaagca
    15121 gcaagaaaat tgaatcgtta tctcgatgaa aaggggtatt atcgcggcag aagcctcggt
    15181 gatatctgtg aagaagcgat gacagagaac aacgtgattt tcgatggata ttttgatccc
    15241 ccgacaacac aactcgatat ggaaacatct gaaggcgccc cgtatgccac ctatgcattc
    15301 gcaacccaca tgacagaagt agaggtagat gataaaacgg gggtctgcct ggtaaagagg
    15361 gtacacgctg ctcacgacgt gggcaaggca gtcaacccgc gactggtaaa aggacaggtc
    15421 tatggtgggg tggccatggg aatcgggctt gcccttatgg aggaattcat tcccggtaag
    15481 tcagaatcac tcgataacta ctacatgcct acttctatgg atatgccgga gattgaggtg
    15541 ttcatcgtag aggatgagga accgacagga ccattcggcg ccaaaggggt gggtgagcct
    15601 gcgctcatac cacaggcagc gagcattata aacggcatac aggatgctgt tggcgttaaa
    15661 ccctcgcaac tgccctgtca tatagagaga ttgaaaaaac tgattgaaga taagaagagt
    15721 gaaaggaggc aggagccatg agcgtgctgg taataaagaa tattggtaca atttttaccg
    15781 gagatattga atcaccatcc atagagggtc cggtagggat tatcatagaa gacggcaaga
    15841 tcaaagcgat tgagaaggaa tctcctgttt tggcagacaa agtcattgat gcaaatggaa
    15901 tgacagtctg tcctggactc attgattccc attctcatcc ggtctttggt gattttacac
    15961 cacggcagag ccagcttaac tttatcgaaa gcagcctcca tgggggtgtg acaagcatga
    16021 tctctgccgg tgaggtccac ctgccgggca gacccactga tccaaaggga acaaaggccc
    16081 ttgcccttct ggcacagaaa tcattcgcaa aagcccgtcc tgccggtgtg aaagtttacg
    16141 gtggcgccct cattcttgaa aagggtctcg tagaagatga ctttaaagaa cttgcagcag
    16201 atggggtgtg gcttgtagga gagattggac ttggcagtgt aaaggcagca caggatgcaa
    16261 aaacaatggt agagtgggca aagaaatatg gaatgaaggt tatgatgcac gttggcggta
    16321 cgtctgttcc cggcagttca actgtaacgt gcgaagactg ccttgtcgca aatccaacag
    16381 ttgcatgcca tcttaacgga ggtcctacaa gcgtgtctgt tgatgaggca cgacaggtga
    16441 tcgacaagac tgaatgtgca atagaagtag tccactgcgg aaaccctttg accgctttcg
    16501 aaatagcccg ctatatgaag aacaaaggga tactgaatag actcatcatt ggaaatgacg
    16561 ctccgtcagg aacaggagtg attcctctcg gtatttggag aatggtcaat tttgtttcat
    16621 ctctttgcgg tatcccagca gagatcgcta tttgctgtgc aacaggaaac accaagactg
    16681 tatatggttt atctcatggt atggtaaagg ttggagacta tgctgacctt cagataattg
    16741 atgcgcctat gggctcagca ggaaaggatg caagggaagc gattgaagta ggtgacatac
    16801 cgggtatagg catggttctt atcgatggga ttgtcaaaac acaggttagc agaaacacac
    16861 cgccgccggt gaggaaagta atagcataga gcgtaagcag agggcatagg gaaagtacgg
    16921 caacagaacc tgcagaattg cagctctaat ctctcggttc tcacatctca gcgaaagagg
    16981 tttattatga aaatagatat agaacgatgt aagggttgtg gactgtgtga agaggtgtgt
    17041 ccgcttaata ttattaccat taaagataaa aaggttctgg tgggtgatgg ctgcgttgaa
    17101 tgtaaaacat gtcagaaggt ctgcccggaa ggtgttttca gtgttataga taatgacgac
    17161 aaaccaatgt gcacaaactg tccgatcatg tgcaggatac cagaaggagc tacaggtgcg
    17221 tgcttgcgct attacaatgc aaagggtgag attctgagaa agggcagggt acacacctac
    17281 gatgaggttc tcaaccttgt tggagcagat gaagattcat tgatacaaaa accactgatc
    17341 acgggtattg gctcaggtac aacatatcct gatttccgac cttccccatt tattgtgaaa
    17401 ggcgtaagag acgggatcga tatagttact gttgtgaccg aagcgccctt aagctacagc
    17461 gggatgaagc tcaagataga cacagacctg taccttggcc aggagtcaaa aaaggtctat
    17521 atccgaagaa aaggcaagag gcacctgggt catctttgta cagaggaata cggttcaaag
    17581 atactttctc ttgggggagt aaacattctt acatccaaag acggactttt cgcagcaaag
    17641 actttgtttg aattccttca gggaaaaata ataaaggttg aggttgaaga cggtcctacc
    17701 ctggaacttg ctcttggcaa agcaccaaaa gttaacggtc aatccgagga aaaaatgagg
    17761 gtagggtgcg gaagtgcagt gtccggcctt tttgcaccgt atatggaaaa ggcagcagac
    17821 gaagttattg ttcttgatgg tcacattaca ggacttttca gcgaacaccc ggcaggcaga
    17881 tatctgaaga agaagcggtc acctgtatcc ataaaaggcc aaaaaagcac tgacggaagg
    17941 tatttcctgg gaaaaggaac agggtggggc ggtaccgata tccaggagcc tcttgatgta
    18001 ataaaagaga ttgatcagaa gaaatgcaaa gaaggcatga ccctccttat tactgaaacc
    18061 actggttggc gatatgctta ttttcgtatg ataaagggtg aatttaggga agagcagcct
    18121 gacgatggag ctatggaatt cataaaggtt ttaagaagtt cctgcgaagt gtcaagagta
    18181 agcgcaattt ttgcagccgg tgtaggtggc tctgcgaggg caggggtaac aaaaaatcca
    18241 atcaaattga caaaagcagt ccatgaaggg aaggtctctc tgacgatcgg tggtgcaaag
    18301 ccttttatct tccctggcgg gggtataaac ttccttgttg atgtagaaca gatcaaatat
    18361 ggtagcgtct atgtttcacc aacgccatca tttattctgc ctgtggagta cacaatgtca
    18421 ctcgacacgt tcaagagcat tggcggccac atcgatgctg taaagccgat agaagaagtg
    18481 ttgaatgaag taaaagataa agcaacttcc tgatctttat catgtttata gaaatcggtc
    18541 cggcaagcct tgtagtatat ggtgaaaaaa agggtatgcc atacgaattt gacagatcca
    18601 ggcttgagga aaagattcaa aatattttga gagatattcg ggagtatctg ccggtactga
    18661 aacaaaaagc atataagatc aaaaacacga aacatatgcc tgatgtgcct cgaaagatgg
    18721 tcgaagctgt taagacaata gatgaaatga gcctgacacc catggcagcc gttgccggct
    18781 ctgtctcaga tgctcttaaa gagcatatca aaggcgaagg gcttgatatt atttctgtaa
    18841 ataatggtgg ggacatctca gtttttgatc gttatgaaaa agggttgaaa gtcggtattg
    18901 gtgacattca cacaggcagg gccacttctt atacccttga cattaaagga ttttccgatt
    18961 acggcctggc aacgagcggc tttggcggaa ggagcctcac attggggctg gcagatagcg
    19021 taacagtagt tgcggcaaca ggtgcagtgg cagatgcagc agccacgttt atttgcaata
    19081 ataccagcat aattactaat caggttcgtc gaataaaggc gtccgagatt gatccgctga
    19141 cagatattcc agaagagtat gttacagttg aaattaatca gttatctgaa ggtgttataa
    19201 acgattcatt ggaaaaagga ttgcaattgg ctttcaagat aaaaataaaa aaatatattt
    19261 atgatgccat tatcaaacta aaagatcata tcgtcaccac gatcaatggt ggctacccca
    19321 ttattttgga ggtgcataat ggaaattaga aagattgtca cagttgttga agagatctat
    19381 gaggaaggtg agaaaaagct cgataagcca atccgtaagg ttgccgcact tgcagtgatt
    19441 aaaaaccctt atgcaggaaa gtaccaggaa gatttgaacg cccttatcga ttacagcgaa
    19501 gagcttggaa aaattatctc tgaaaaagca gttgaagcac tcggtaaaga tgtggaagtc
    19561 cacagttatg gaaaggcggc aatagtgggc gagaaaggag agctcgagct tgcggcggcc
    19621 cttttacacc caaagctggg aacccctctc cgtgcagcta ctgggggtgg gaaggcaatc
    19681 attccttctg tgaagaagtt gggcagtatg ggagacagcc tcgatattcc ccttcattac
    19741 aaggatgctg cttttgtccg gtcacatttt gacggcatga cggtgtcaat cagcgatgcg
    19801 ccaaaatcgg atgaaatcgt tattgcagtt gcagttactg atggcggcag accccatccc
    19861 cgtgtcggcg gactcaagaa agatgaagca aagaaggaag acgggctgag ataggccaat
    19921 acaaggttaa tggtgcaagg tgaacagtat ttcctgttga aagacccggg gaaaatcggt
    19981 ataatcaacc ccgtgccccc gtagctcagg tggatagagc aatggattcc taatccatgt
    20041 gccgcatgtt cgagtcatgc cgggggcact ttactatcaa taagttacaa atcatcttca
    20101 tgcaggcctt tacgaacagc taaatgagtg cttaccgacc ccaaggttct cccatcccta
    20161 ttcctgtagc cggcaagcca gtcatggatg gtctgcctac ttacgtgaaa ttccctggct
    20221 accatttcgg ggttctttac acctagcagc ctcaccactg ttccttcccc cagttccatc
    20281 agcgtttcgt gcttcaatag ccacgcatcc attgtgctgg caagtacaca ataacagacg
    20341 cagaaatgtc aatgcaattt atggaataat taatctagga ctccacacgg cgtaatccaa
    20401 atatcatagg gcactttttt gggaatctta gcactccaga gaaaattcca tgtatcaaat
    20461 ccggttgggt ccgagtacgt gccatatccg ggtactgatg tgaacttatt gtttattaca
    20521 gttacataag caaagtcata ctcataacca tcctcagaaa aaaactctac ataaccaaaa
    20581 aagtttgagt tagttccgga tagccaataa agattaccgt atatatctgt atagtttccg
    20641 cgtctatcat atgatctaag ttttatagtg tctggagcgc caggttcaac ttccatgcat
    20701 acatagctct tataagactc atttccaccc tttgtaggga caatgtttga gccgtcaata
    20761 ccggcattta tcatgttacg gaattttgtg gaatcctgca accataagcc gtcaaacgaa
    20821 gtcaggttta cctgagcctg aacagtatag gcgccaatca tcaaaagaat tgctaaaaaa
    20881 attgtgatgc agagtgattt tttcatgtga aacctcctca taaataattt tttgtctgct
    20941 gtaagtgatt tacagctctt ttcatgtacc cttgcttctc ataccttcca atatggcaca
    21001 gcactattaa acagaatatg tatccgagag tcaatacaca tatcgatgag ggagcaagct
    21061 tgtgtgtgtc tcagccgaaa cgaccttacc cctgtaaagt gtacaaattt ctttagagaa
    21121 ccagggtgtt cactctccct gtttatcaag gaattgatct gtttccacaa gagcaggtca
    21181 atataaaatt tgcttggaaa ttgggtccat atggtttttc tccctcttgt tctgtgattt
    21241 gtataaattt aaaaagctgg tttcatgtta aaattggagc tatgtcaggt gattgggtca
    21301 gcgggttctc tccataacga ttgtgatctt caagggatat ggtgaaggaa gccttcacca
    21361 agggattggg gagatcttat cgccggcgtg tccatgaagg ccaatgcctg ttcgtcttcc
    21421 ttgtaaatcc agtataatct gtcctgcttc attgaccggg aaatttaaac ggacgggcta
    21481 ttcaaaaatg gctttggtag tgctattgaa aatggattat gctaaccttg tgcaacagga
    21541 aacaaggcca ttggttaaac tcaacagaaa ggaacctcgc cgtggtaaag ataacgcatt
    21601 ccaaatatgg aatggaatga gcagcagggt acacaagtga caggccgtaa ttcgcttcaa
    21661 tacaagtggg tgatcttcat tgtgcttgca gtccaatacc tttttggcta tttccaccgc
    21721 gtttgtcctg ctgttgtagc gcctgaactc atgaagtcat ttgatataag cggcatggcg
    21781 ctgggggttc ttgcctctgc atatttctat ccctatgcag caatgcagat accggcaggc
    21841 attctctcag atgcctgggg agctaaaaaa acagcaataa tgtgcgccct tattgcgggc
    21901 ataggatcgg ttcttttcgg tctttcccac accctcggtt ttgccatttt ctctcgcatc
    21961 cttgtaggcc tcggtgtttc tgccatgttt gttacatccc tcaagatatt tggcaactgg
    22021 tttaaaccta ctgagtttgc ccgtgtttcc gctctttaca ttgcagcggg aggggtaggg
    22081 tggttgacgg ctgccactcc cctggctatt ttttcacagt atttcgattg gcggtgggca
    22141 tttattgggc tcggaataat taccctgctc cttacctctc ttatgaggtt caccgtttca
    22201 gacagacccg atgaaatcga tcccgggact gttgtcgaca caggcaaggt tcagggaaac
    22261 agtatggtga agattggccg agatctgcaa atggtcatga aagaaaagta tttttggcct
    22321 ctggctgcct ggatgttttt tgtaggcgcg ggtagttttg gcttcgtaag cctgtgggca
    22381 ggtccttatc tgatggatgt ctaccggttc tcaaagcctg ctgtgggaaa tatcctttcc
    22441 atgtttcctc tcgccatggt ttttgccgga ccttttctgg gttatgtttc cgacaaagtc
    22501 ctcgtgagca ggaaaaaggt catagtggca acttcaatag tgtacgttgc gtgctggctc
    22561 atgatgcttt tttgtcacga ttcactcccc ctttgcttgc tttatttgtt ttttttcttc
    22621 ctgggtgcta tgggcacagg tgttttgccc ataggatatg cagccacaaa agaacttttt
    22681 actggagaag tagcaggcat ttcaattggc gcggtaaatc tctttccttt tctcgggggt
    22741 gtggtagccc agcccctaat agggtttatc cttgacaagg gggggaagat tggaacccat
    22801 tatccatcct ctgcctacca ttcagtgata ctaatgtacg ttttcataag tatcttgaat
    22861 ctcattgcag tttttctcac aaaggaaacg ctcagaaagc agtgacctca gaggtaagac
    22921 cgtatgttca aaaagcagac aacagaatat tttaccggca aagaagcctg attccaagcc
    22981 atgctccacc tttttgggcc ggggaggtta aaataaaaat tcatgaaaag gtctggtatt
    23041 tccttattga cattttcttt atattcgttt aggaatgttg aacacattaa aaggaggtga
    23101 tgaggatggc cgcaaagatt gacgaagatg cctgtacagg atgcgggaca tgcgctgatg
    23161 catgtccggt tgaagccatt accgttgatg atgttgccaa ggttaatgca gatctttgca
    23221 ccgactgcgg aacatgcatt gacgagtgcc ctgtagaagc aataagtaag gctgattaaa
    23281 cgtttgaaaa ttgctcaacg caagacctca tgcaatccgc ccctctgctg tgtgaataca
    23341 agtacgggcg ggttaccccc gcagatcata taataacgga atatatgaaa atcagagact
    23401 atgataggct tgagaagttt taccgagtgt gactgtatgg ttaagccact gttattactt
    23461 cataattgat ccattgtcat aggatgttaa atagatggca gtatttttac aaaatagtac
    23521 gattaaaaat gtatactgtt ccacttattt attcttaagt ttcatcaatc ataagcgaca
    23581 ataaaaatac agaacattcc acgggcctca aggcattgct tgtcctaccg tagcttaccc
    23641 ctcggaaagc agctttgggg ccttaagttt attttgattg tttccccccg cccttacctt
    23701 tccagttctt cacgtcccat cccttacttc acatcaacaa tttccagctt atggtttgct
    23761 ctgggatttc gcggagatgg tccttcaata agaatcacaa agtctccgct taggctctga
    23821 gatttaaccc attctctggc gtattctgtc caattatgtt tgttctccgc ttcaagaatg
    23881 gggtgcactc cataagaaaa ctgaagagcc tgacacgtgg catcttgtga tgctactgca
    23941 gctatccata tcggtaatcg gagacgggta agcctttggg cagtacctcc gctgtggctc
    24001 ggtattaata cagcggtaac aggcatttgt tgaagcgcac tctctacaga caaagtaata
    24061 aggtcacctc ctgtaacttc attgccggtg acaataactt tcaaagcttc tctaacacga
    24121 tggccaggcc gagaaggctc aattgcaacg gctattttaa ccagcattgc cgtagattcc
    24181 acaggaaatt ttcccatggc agattcacca gaaagcatga cacaatctgt gccgtcaaga
    24241 atggcattgg caacatccgt tgcttctgcg cgagttgggc gactattatc tgtcatagac
    24301 tcaagcatct gagttgcagt tataaccggt ttgcctgaca tattagcttt ccgcatgagc
    24361 tgcttttgaa caacagcgat tctttcaata ggtatttcaa cgcccaaatc acctcgggca
    24421 atcattatac cgtcggcagc ttccagaatt tcatcaatat tgttcaaagc acctgaacgt
    24481 tctatttttg caatgataaa aggaacgcgt ccacatgctt tggcagcttc ccgcaccaac
    24541 acgatatcag attt
//
LOCUS       DAOOMC010000027        23841 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdus sp. isolate AMR_MDS_2685
            NODE_2010_length_23841_cov_5.613218, whole genome shotgun sequence.
ACCESSION   DAOOMC010000027 DAOOMC010000000
VERSION     DAOOMC010000027.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864760
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdus sp. (wastewater metagenome)
  ORGANISM  Syntrophorhabdus sp.
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae; Syntrophorhabdus.
REFERENCE   1  (bases 1 to 23841)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23841)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,729
            Genes (coding)                    :: 2,708
            CDSs (with protein)               :: 2,708
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 7 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23841
                     /organism="Syntrophorhabdus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2010_length_23841_cov_5.613218"
                     /isolate="AMR_MDS_2685"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2699756"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            131..1357
                     /locus_tag="PK125_04270"
     CDS             131..1357
                     /locus_tag="PK125_04270"
                     /inference="COORDINATES: protein motif:HMM:NF002674.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tm-1-like ATP-binding domain-containing protein"
                     /protein_id="HPB37356.1"
                     /translation="MKKQLLIIATLDTKGRETEFVRNCAQTLGVNPIIMDIGVLGDPP
                     IAPDISKMDLAAATGVNLPDLIQKKDRAKSVQAMEDGGAILAQRLRNEGKLDGIIGLG
                     GGTGTAIVSSIMRTLPFGLPKVIVSTVASRDIRQFIGTKDIVMFHSVADLLGFNEFIR
                     LILKQATYAVCGMIEGTSALGKDKPMIGVTAYGINSGVALNAEPFLVSKGYEMLGFHA
                     NGCGGMAMEELVAEGRIAGVLDFNPHEIADEMFGGYCKGIGPSRLETAGRMGIPLVFA
                     PGGLDNAVFSPVYPMPESLKGRRIHGHDTRFCVRMEQEEMKAFARIIADKLNKSKGFT
                     HVLIPLKGWSDADKEGAALYDPSVDEVFTKTLKELLLPLVPIEEIDLHISDPNFALRA
                     VDILHAMIQQKITQGE"
     gene            1360..1782
                     /locus_tag="PK125_04275"
     CDS             1360..1782
                     /locus_tag="PK125_04275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB37357.1"
                     /translation="MTTAHEEIPPPVVRFFDVSESEGRFKVLARVFEIGPDCLVILWG
                     GTRPHIGAVGLAQVRPSLRDSNKRAASSSVFTFVGHKEDMVAKMMSEEIAKRLGRNTV
                     VSAGIHWDNLTDVEIETITKLSRSVLEDIVNKFDTVFC"
     gene            complement(1788..2699)
                     /locus_tag="PK125_04280"
     CDS             complement(1788..2699)
                     /locus_tag="PK125_04280"
                     /inference="COORDINATES: protein motif:HMM:NF015431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HPB37358.1"
                     /translation="MYTYSMNSLLVFYEVIKARSFSKAADVLFMTQPGVSNHVSQLEA
                     QAGHRLLKREKGKFELTKEGKVVFKYAEKIEALATDLEHAIRNLRKDVKPLLRIGSPP
                     VYSRIVMPYILSSFQKTYPDTMIRLDTGASEEMLRSVLSMHNDIVIVANTRASKKLYV
                     LPFIKEELVLITAKKHPLAQKDSVHLKEVEPYPFILREEGSGTRNTILGAFQSMNIKP
                     SSFIEVKSTDFIKEWVSHGKGVSILVKRAVADDELDSLKIVSLKERLSMEISVCFLKS
                     RKNDRVIQGFSDHVTELSQKPPFKAGG"
     gene            2792..4204
                     /gene="ppcB"
                     /locus_tag="PK125_04285"
     CDS             2792..4204
                     /gene="ppcB"
                     /locus_tag="PK125_04285"
                     /inference="COORDINATES: protein motif:HMM:TIGR02724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylphosphate carboxylase subunit beta"
                     /protein_id="HPB37359.1"
                     /translation="MDLRDFVAKCEMVGQLKRVKAEVDWDLEISHVAKVVEEKSGPAL
                     LFEKVKGYTSPVLTGAFGTTQRLAIILGKDPNLSMVQLTREWVDTAVKALTPAKEVKD
                     GPIFENILEGDKIDTFAFPSPKFYELDGGRYFGTAVFMVIQDPETGDINLGTYRMGIL
                     DEKSVGVQILKGKKADRIMKKYAKQGKKMPACAIIGGDPLHIFAGAATIKAKSGYDVV
                     SSLRGEPVEIVKGQVTGLPIPAAAEIVLEGEIDPTQLRNEGPFGEYTGYYTEELIKPI
                     PKPALDVKRIYYRNNPILWETSVGRPVGDQHILYAFTRNASLWTDLTKMEIPGIKSVY
                     TLPEGSGRFIVVVSVQQMYHGHADQIGAAVIASNTGTYGIKMVIIVDDDIDADDLPRV
                     WWALGTRYNPLRGTQLINRGRSTPLDPAIGADENKFITSRIILDATIPFDWKIKPTEI
                     ALNQDMFNKVKARWKEYGID"
     gene            4221..4778
                     /locus_tag="PK125_04290"
     CDS             4221..4778
                     /locus_tag="PK125_04290"
                     /inference="COORDINATES: protein motif:HMM:NF019887.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HPB37360.1"
                     /translation="MAFEKDINNARERARKVKLFGHDIHGTLTTNSVIVDIEGNRRYG
                     FWHMDGFADLSLTANDIKVAFLDTTSIDGEGLFRAKELKLDKFYYSIKDKSAKIEELT
                     KEMGISLDEVGFLGCEVSDIPVMRKVGFGVATADARDEVKALAAYITTNPGGRGAMRE
                     VCEFILRAMGKWDEWVGKVTKMGYK"
     gene            4789..5325
                     /locus_tag="PK125_04295"
     CDS             4789..5325
                     /locus_tag="PK125_04295"
                     /inference="COORDINATES: protein motif:HMM:NF019887.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HPB37361.1"
                     /translation="MAIEKPDAKEKAKKVKFVILDIHGIMTDNTLFYTQEGIKSEAFS
                     LHDRIGIKALTDEGIGVSFLTTKISRADEQMAKIYGLPPENLWGQGAKMARVDQFEQE
                     TGFKDEEICYAGDEMIDLAVMKRSGFSVAPADGSQEARDIADYVTTAGGGKGVVRELA
                     QFILEAQGKWDNFLGEFK"
     gene            5344..6783
                     /gene="ppcA"
                     /locus_tag="PK125_04300"
     CDS             5344..6783
                     /gene="ppcA"
                     /locus_tag="PK125_04300"
                     /inference="COORDINATES: protein motif:HMM:TIGR02723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylphosphate carboxylase subunit alpha"
                     /protein_id="HPB37362.1"
                     /translation="MAYKYYKDNRAFIEACVKTGDAVVINEEVDWDLEMGAIVRRTCE
                     KKGPAVFFKNIKDYPGFECVGAPIATYRRLAIAFGLDPDTPIPEISKEYLRRTTGKSY
                     PPKVITNAPCQEVILQGDDANILNLPAPMVHDGDGGRYIGTWQFVVAKDLDTPFINWG
                     MYRQMAFDEKTMAGPVLPFSDMGKMWHNKYQPKNIPMPFAVVVNPDPLSAIASCAPAQ
                     IAEDEFTSQLMGEPVEIVKCVTCDLYVPAHAEIIIEGVLVPNVTIDEAPFGEYTGYRT
                     SPREPRTVYYVKAITMRKQPIMAMSNMGVPTDEGQLLRSFSLGLEMDKMLRDQGIPIT
                     GVHMLPESTHHLTVVGVKPAYPNIATQIGQLVFGSKLGPWFHMVVVVDEKTNIYDKDE
                     LIHALCTKCHPVNGIRVEPFGVGTPLNPFATPAERRLGVAGKVIFDCTFPLDWEASDI
                     PIKVSFDNVYPKDIQEKVLAKWKTYGFKE"
     gene            6793..7047
                     /locus_tag="PK125_04305"
     CDS             6793..7047
                     /locus_tag="PK125_04305"
                     /inference="COORDINATES: protein motif:HMM:NF021199.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylphosphate carboxylase subunit gamma"
                     /protein_id="HPB37363.1"
                     /translation="MAEWDTYINVNFKDMPEEIEQVTVIRDLTPGKYKYRSTYAKIIV
                     SKDPEKYPEKVWVRLGRGQLIPTPCSMKILEFVNIIPKGL"
     gene            7049..8383
                     /locus_tag="PK125_04310"
     CDS             7049..8383
                     /locus_tag="PK125_04310"
                     /inference="COORDINATES: protein motif:HMM:TIGR00148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HPB37364.1"
                     /translation="MVTSLKEYLDIVGSKNWVLTIREKVYLEDIPALIEKLSWEGKIL
                     LFEDIEGYDCKLAANLVPSHDIFKLLFETENPYEYFIKGVQQRVKQIHVAVEGFKHME
                     VSGKNLLDVIPILKHYEKDSAPFITTGIISSVDPDSGIVGRGIHRMEYRGENLLGVTL
                     LNPPLTTIHDKYLSSGKRMPVAITIGVDPALFISMALKAPSGTDKLEIAGGLKGKGIQ
                     VWQSPQSNIEVPASCELLLEGYIDESRSKKDGPLGEISGYYLSLEKTPTIVVQHMYYK
                     ENLIYHALLPTSPESDRYLTFVSRAHLEESAKALYPFIVNLTFVPKTFGSSLVVNVRT
                     SEKFKIRNLILFLLSFPMIKKVVIVDDDVNPDDPYDVEWAIITRCRADEDFIIVNKLQ
                     GQPIDPTVEDGYGITKVGINATIQGKMIQERAKVMRGNEKRIEVVLSKIDGR"
     gene            8387..8977
                     /locus_tag="PK125_04315"
     CDS             8387..8977
                     /locus_tag="PK125_04315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018036727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HPB37365.1"
                     /translation="MSKIVVGISGATGVIYGIRMLKTLFQLGVETHLVVTSSAIKNML
                     IETEYTMADLEPFASTIYDVDDVGAAIASGSFQIDGMVIAPCSIKTLSAVANSFNYNL
                     LIRAADVNLKERRRVVLLVRETPLHEGHLDLMMKITRIGGVIMPPAPAFYHMPKTIDD
                     IINQSVGKVLDLFGIDASLFKRWGSNTKEEIKIVKT"
     gene            9078..9455
                     /locus_tag="PK125_04320"
     CDS             9078..9455
                     /locus_tag="PK125_04320"
                     /inference="COORDINATES: protein motif:HMM:NF014084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP-dependent riboflavin kinase"
                     /protein_id="HPB37366.1"
                     /translation="MKITGKIVKGLGESAGFIAIPWVTKQMREKLKFQPFGGTLNLAI
                     EDPEIQRSLKAHGGDRLCSEAAGFCDALIFRGKVAGKYECGIVVPLVPNYDEHLLEIL
                     APVHLKDSLGLKDGDEVTVELYL"
     gene            9617..11344
                     /locus_tag="PK125_04325"
     CDS             9617..11344
                     /locus_tag="PK125_04325"
                     /inference="COORDINATES: protein motif:HMM:NF012863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HPB37367.1"
                     /translation="MGKQVFLLSGILFVLWAGFGFGDETSATENLIQGATLPTRVVGS
                     IAAIGDSITAAFNAAYSEFESCKFRDTPAYNFATNKSKNTTFSIAERVMAYKGDIVAT
                     ANFGQDGARMSHGDDQAKAAKTWLMKQAAPHLVIVFLGHNDICSGTKDKFSTCESANR
                     DPNNYCRTSFFAYEQQMRQMLDVLITIPDSQILIIHPIRVSQLCNFRNEKVIDVPWTS
                     KKCGDLWDAPMNIFGQKGICPSLTDCTAGRIADAYTTWVAYREIADRLVDEYNSYTAG
                     DTIPANDDFGTGSVVRAPDISLVATNVIGNSRFQYRDTFGNPQLSVCECFHPSKYGQN
                     LLANLLWNGVTCSEKTPCCDDSIESDTDYDRGLCKSYSTSGSMTGPWTILDTYKILKV
                     EKIGSGTGVVASTSPGIDCGSACDASFLRGSTVKIRARADADSVFKGWSGACSGSAKA
                     CTLGMSEDRVVTATFLSDPTIKVTPTRKNFKKVKLAMNRVMIFTIRNKITKGKKDLFV
                     GTITSDNPVFVPVDDLCSNTSVSPKRSCKFKIQFSPVVGHHTAKITIPTNDPQATNTE
                     IILIGEGIP"
     gene            12192..13946
                     /locus_tag="PK125_04330"
     CDS             12192..13946
                     /locus_tag="PK125_04330"
                     /inference="COORDINATES: protein motif:HMM:NF015948.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HPB37368.1"
                     /translation="MSEIILKKGKLSSTLLRGLVDYYAAIPGVDKSYVTLRWDQWKQG
                     DNIAVYLLKKDEEPVGWIIYNRATSTIEEILLNPDKDQALVRFQAIDALIDRESLLSA
                     EILEEDTEKFYWLGEYGFRPTRKIQVFGQTLIKMELSTVVFFQHLKEHKPAKPYRKKE
                     KVVIEQVPSPQSESEIKASLQDLLNKLGGIKKYVKPGQTVVIKPNVVADHGMLGGKYT
                     GGVVTDIRVLKGLIELLLPVAGKVIVAEGSSINRSATVKMFEIYGYPKLIDLDPKKVS
                     LVDLNTDQLVEKLVPAGKRMKSRKVPRTIEEADIVISVPVMKIHFAAGVSLGVKNLQG
                     AMPPLEKYMTHFFGLWQNLVNIHHVVKPKLTIIDGIVGQEDFGPVSGTPKTMNLLIGG
                     ENPVAVDAVTMRVMGLDPHISPPVLLAYMQGFGPIEPENIEVLGTPIDKVAKPFKQPF
                     LNLESGKYFKVHGTDACTGCRGYLHFALNKLRRPDPADPSRLLIDRPFEPKVNIYLGP
                     YEGANADPKETNIFMGICQLHHTENGMSLVGCPPHAEVIMNGIFSLFPDVERPKYADD
                     TEEAKLERMLKEALATLA"
     gene            complement(14089..16221)
                     /locus_tag="PK125_04335"
     CDS             complement(14089..16221)
                     /locus_tag="PK125_04335"
                     /inference="COORDINATES: protein motif:HMM:NF012602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HPB37369.1"
                     /translation="MAMQVIKSDCIMCINSCGINAYVEDGKLVKVEGMKEHPISLGEI
                     CPRGTALPEWVYSKDRLTFPMQKKTDGNWERISWDYALDTIAEKLQEIKDKYGARALA
                     VYTGSLGTENIELAAFAQRFRGVYGTPNLLSVEGNCFRSRIMARQMTFGGYPIEEPWN
                     SKCIIVFGQNMDNSRITVGRKIYKAMDAGTLKHVIIVDPKKIPMAERGIHIQIRPGTD
                     TALCLGMLNVIIEQGLYDKEFVEKFCIGFDKLSEHVKQYTPEKMSEITWVPAEDIRRI
                     ALLFATVKPASIVPGTCSIDQHVNGFQGNRVHAIMQAITGNIDVPGGWVSIPFIRLGD
                     MRVTEISDPIGTDEHPLFRRFWGRTSPYGQQMLFADAVLKGKPYHIKGMICTGGNPAV
                     TLPDSERIKEAIKALDFVVVSELFMTETAKLADIVLPACSFLEKAGVGYVYGVTNCIP
                     YAMVRKKVIEPIGECWPDWKIWTEIGRRMGYGEFFPWNTEEEIIDFFLKPSGLNREQL
                     TKEHPEGAYYTEMKYVQQKRYNTPSGKIELYSQTLAENGYDPLPKPVEPSKSPVSNPE
                     MYKKYPVILTTGARIPEYTHTQLRDVPYVHRTAPEPLAEIHPDTAAQYGIADGDMMSV
                     ETPKGKIFIKAQTTDKLLRGVLSVPHGWDKANANVLTELEPRDPVTGYTEMKALLCRI
                     SKVAQEVVESTTEVEIASDCFPSGVCAT"
     gene            complement(16236..16847)
                     /locus_tag="PK125_04340"
     CDS             complement(16236..16847)
                     /locus_tag="PK125_04340"
                     /inference="COORDINATES: protein motif:HMM:NF019958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HPB37370.1"
                     /translation="MSKKDMRRQQIIRAAAEIFGNSNFQNASIAEIAQKADIAEGTVY
                     QYFKNKEDLFFSIPAQKTEEFCEEFNLHLQGIHDGVGKIRKLIWYYLYFFKSNPAYAR
                     SLMLEMRVSRGFIKSETYGKIKAFTEKVLEIIKEGQESGLFRKDTSAYTIRELILGVL
                     EQRVTRWLLKEETYDLIEGYEEVCDLIFNGIKSSERVEGRNII"
     gene            complement(17286..17879)
                     /locus_tag="PK125_04345"
     CDS             complement(17286..17879)
                     /locus_tag="PK125_04345"
                     /inference="COORDINATES: protein motif:HMM:NF024900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HPB37371.1"
                     /translation="MSDILLTIDGKEVRAKEGMTVLNAAQSSGIFIPTLCHHEKLEPY
                     GGCRICTVEVESRGRTNYVASCLYPVEKNLVVRTRSEKIDKIRKVLIELLLAHAPDSP
                     QLLEMAKEYGAKSVRFGKEPSFCVLCGLCVRYCAEVKKKNAIAFVDQGPKREINFIPE
                     IASKECWNCKECFPLCPTSYLQAAYVLTQGLVGFKNS"
     gene            complement(17927..19582)
                     /locus_tag="PK125_04350"
     CDS             complement(17927..19582)
                     /locus_tag="PK125_04350"
                     /inference="COORDINATES: protein motif:HMM:NF013662.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-ubiquinone oxidoreductase-F iron-sulfur
                     binding region domain-containing protein"
                     /protein_id="HPB37372.1"
                     /translation="MARINSAAQLEKVRKDIVLKKDPNKPCIAICGGIGCLGGREDLI
                     RAFEKEIKKQKLEGKVDVRVTGCHGFCAQGPNIVIYPEEICYTKVKPEDAQQIISETV
                     VGKTIVDRLVYIDPNTGEKAVHQSEIPFYKKQVRNILGMNPKIDPKSIDDYLAIGGYS
                     SLAKALLKMKPQEVLEEVKKANLRGRGGGGFPAGLKWETARNAPAELKYVICNGHEGE
                     PGAYMDRAIFQGNPHLIIEGLIIGGYAIGAKQGFIYTRHDMPRLRENIDIALEAAREH
                     GFIGNDILGSGFDFDIEVHLDVGIFVSGEASALMRSIEGKAPEPRPKYTRTAVKGIWE
                     RPSNLNNVETWANVPQIIGKGADWYLGIGSEGSKGTKMISLSGNIVNSGVVEVPFGTS
                     LKEIVYDIGGGIPNGKKLKAVHFGAAMGGSLPAELIDTPLDFDVMSKLGAAIGAGGLF
                     VMDEDTSMVEMNRFFLEFLADESCGKCVPCREGIRQMLRILTRMSMGKGKEGDIEALE
                     ELCEVGSLASLCALGKTTSSPIYSSLRYFRNEYEASIEDKGCV"
     gene            complement(19592..20044)
                     /locus_tag="PK125_04355"
     CDS             complement(19592..20044)
                     /locus_tag="PK125_04355"
                     /inference="COORDINATES: protein motif:HMM:NF013426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HPB37373.1"
                     /translation="MDNRRIDEIINKYDGKPNSVIMVLMDIQHENKWLPREVLEKVSN
                     KLGVPLSEVLHIATFYKTFSLKPQGRHEVHVCTGTSCHQRGSAKILEKVQDLIGIKPG
                     ETTPDLRFSLEKGSCLGCCTLGPEIVVDGMHHSNITPDDVEAILKNYK"
     gene            complement(20070..20861)
                     /locus_tag="PK125_04360"
     CDS             complement(20070..20861)
                     /locus_tag="PK125_04360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012645317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(20805..20807),aa:Sec)
                     /transl_table=11
                     /product="hydrogenase iron-sulfur subunit"
                     /protein_id="HPB37374.1"
                     /translation="MSNGFEFKPKILGFICFWUGYGAADLAGVSRLQYATDIRLIRVM
                     CTGRVDPAFIVRAFSNGADGVYIGGCWPGECHYVTEGNYHALSNVLLMRRILTHIGLN
                     PERLRIEWVGASEGVRFAEIMNDVSKKIKELGPIGQAEGIEPKKLAFKFEAVNNIVPY
                     MRLVERERMRINLNSEEEYRKFYSSEEFDELFKELITDKLAVSEIMLLLREGPRSGEE
                     ISEILGLKPSEVSKHLNLSARQGLLRFDESQRVVLPQMREDQARA"
     gene            complement(20897..>23841)
                     /locus_tag="PK125_04365"
     CDS             complement(20897..>23841)
                     /locus_tag="PK125_04365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014809768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HPB37375.1"
                     /translation="GHMAQLDKTFPTNDCSMCIESPKFVECSRHPNIEILTYTEIEKV
                     TGEAGDFKVALVKKPRYIDEDKCTGCGVCVEYCPVQVEDEYNQGLSKNKAIHIYFSQA
                     VPLVTYVDRDKCSFLQDERCTICQGVCKNNAIDLFQKPESLEIEVGAIILAPGYDAFD
                     PATLRDTYGYGKFENIVTSLDYERILCATGPYEGEILRSSDKKHPHKLAWIQCVGSRQ
                     VIEGGNSYCSAVCCAYTQKQVILTKDHDAEAECTIFHNDIRAFGKDFERFYQRAEKLP
                     GIRFIRSYVSIGKEDPVTKNVTVRYSTPDAGVKEEEFDMVILSVGMNPPKDAAFMATK
                     FGLELNEHGFCRVSPVNPIQTSRPGIFISGAFQGPVDIPESVMTASGASSQCGQLLDY
                     RRNELTKEREYPQERDASKEEPKIGVFVCHCGANIGRVVDVPAVVDYAKNLPNVTYAQ
                     EALFACSTDAAKQISETIKNKGLNRVVVAACTPRTHEPLFRDTLREGGINQYYYEMAN
                     IREHCSWVHSKEKEIATQKAKDIVRMSVARSALLEPLEEFELPVDKRALVIGGGVAGM
                     TSALSLAEQGFEVFLLEKEPELGGMARRIYYTLEGLDVQAYLSDLISRVYRNPLLHVF
                     TDAIIHDVHGYVGNFTTRITSGSMAKEIRHGVAVIATGSEEYKPVEYHYGQDDRVMTN
                     LELEGKIVNEDENVMNAKNLVMIQCVGCRNEDRNYCARICCSQSIKNALKLKEKNPDM
                     DIYILFRDMRTYGFKEDYYREAAEKDVRFIRYEPQDPPHVEPGKSEEGKPVLKVTTID
                     YILDKKLELDADIVALAAAAIPSSTTAEIARFYKVALNPDGFFQEAHVKLRPVDFAAD
                     GVFLCGAAHYPKHLPEAISQAYGAAGRAVTILSKDTVVASGAVCEVNENDCVSCGACI
                     SVCKYGAIEFRDTPKGKKAYVNPVLCKGDGLCNPVCPTRAISLKHFTDEEIFQQIDEA
                     IQMNGRAR"
BASE COUNT         6592 a         5616 c         5188 g         6445 t
ORIGIN      
        1 ggcaaaagtg cctgcattcg gtacacaacc cctgtggggt actgtggtta gtatcattgt
       61 ttaaataaga aaacagtcat tttcatatga ctcgataaac aatttaataa aacaagaatg
      121 tgagggaatt atgaagaaac aactcctcat tattgcaaca ctcgacacaa agggccgtga
      181 aacagaattt gtccgtaatt gtgctcaaac ccttggcgtt aatcctataa tcatggatat
      241 aggtgttctt ggagacccgc ccatagcccc ggacatatca aaaatggatc ttgccgcagc
      301 aacaggggta aacctcccgg atctgataca aaaaaaagat agggcaaaat ctgttcaggc
      361 tatggaggat ggtggtgcaa ttttggcaca acgtctccgc aacgaaggta agcttgatgg
      421 tattatagga cttgggggcg gaacaggaac agcaatcgtg tcatctatca tgcgtaccct
      481 gccctttggt cttccaaagg taattgtctc caccgtagca tccagggata ttcgccaatt
      541 tataggcacg aaagatatcg tcatgtttca ttccgttgct gacctgctcg gttttaatga
      601 atttattcgc ctgattctga agcaggccac ttacgcggta tgcggcatga ttgaaggtac
      661 cagcgccctc ggaaaagata aacctatgat tggcgtaaca gcctatggta tcaactccgg
      721 tgttgcatta aatgccgagc cttttctcgt atcaaaagga tatgagatgc tcggattcca
      781 tgccaacggt tgcggtggta tggctatgga ggaacttgtt gctgaaggca gaatagcagg
      841 agtccttgat tttaaccccc acgaaattgc cgatgagatg tttggcgggt actgtaaagg
      901 tataggtcct tcaagattag aaacagcggg ccgcatgggt attccccttg tttttgcgcc
      961 cggtggcctc gataacgctg tcttcagccc tgtttatccc atgccagaat cactcaaagg
     1021 caggagaatt catggccacg acaccagatt ctgtgttcgt atggagcaag aggaaatgaa
     1081 agcctttgcc cgcattattg cagataaact gaataaatca aaaggattta cccatgtcct
     1141 tattccattg aaaggatggt ctgacgctga caaggaaggt gccgcactgt atgatccctc
     1201 tgtagatgag gtttttacaa aaacactgaa agagctcctt ctgcctctcg tccctataga
     1261 agaaattgat ttgcatataa gcgatcccaa ctttgcattg cgggcagtag acatacttca
     1321 tgccatgatt caacaaaaga ttacacaagg agaataaaga tgacaaccgc ccacgaagaa
     1381 ataccgcccc ctgtagttcg attctttgat gtatcagaaa gtgaagggag attcaaagtg
     1441 ttggcaaggg tcttcgaaat tgggcctgat tgtctggtaa ttctttgggg aggcacacga
     1501 ccccatatcg gagcagtagg gctggcacaa gttcggccaa gtttacgcga ttcaaataaa
     1561 cgcgctgcgt cgagctctgt gtttactttt gttggacata aagaggacat ggtagcaaag
     1621 atgatgtcag aagagattgc aaaaagactt ggaaggaata ccgttgtttc ggctggcata
     1681 cactgggaca atctgacaga tgttgagatt gaaacaataa cgaagctgtc ccgatcagtt
     1741 ttagaagata ttgtaaataa gtttgatact gttttctgtt aaactcatca gccgccagcc
     1801 ttgaagggag gcttctggct gagctctgtt acgtgatcag agaatccctg tattactcga
     1861 tcattttttc ttgattttaa aaaacatact gatatttcca tagacagtct ttctttcagt
     1921 gaaacgattt tcaatgaatc caattcatca tctgccactg ccctcttgac aagaattgac
     1981 accccttttc cgtgagacac ccattcttta ataaaatctg tgcttttaac ctcgatgaaa
     2041 cttgaaggtt taatgttcat ggattggaaa gcaccaagaa tagtattcct tgtccctgaa
     2101 ccttcttccc gcaagataaa cggatagggt tcaacctctt tcagatgcac agaatctttc
     2161 tgagcgagag gatgcttttt agcagtgatg agaaccaatt cctctttaat gaatggtaaa
     2221 acataaagtt tttttgatgc cctcgtattc gcaactatta cgatgtcatt gtgcattgac
     2281 aagactgatc tcaacatttc ttcggacgca cctgtgtcca gtcttatcat ggtatccgga
     2341 taggtttttt gaaagctgct taatatatag ggcattacta ttcgggagta aacaggaggt
     2401 gagccaatcc tcagaagagg cttaacatct ttccgtaaat ttcgtatggc atgttccaaa
     2461 tctgtggcca gcgcttcaat tttttcagca tacttgaaga ctaccttccc ttccttagtc
     2521 agttcaaact tccctttttc ccgttttaat aatctatgac cagcctgggc ttcaagttgg
     2581 gaaacatgat ttgaaacacc tggctgagtc atgaaaagta catcggcagc ttttgaaaaa
     2641 ctccgtgcct tgatcacttc atagaaaaca agaagggaat tcatcgaata cgtatacata
     2701 attttttctt ataaagtata agaagaaata aattgacaag tcaagaattt aacgtagaat
     2761 tgaataaata tcacaagaaa gggggcaact catggattta agagattttg tagcaaagtg
     2821 tgaaatggta ggacaattaa aacgagtcaa agctgaggtt gactgggacc ttgaaatatc
     2881 tcatgtggca aaggttgtag aagaaaaatc aggccctgct ttattatttg agaaggtaaa
     2941 ggggtataca agcccagttt tgacaggtgc attcggcact actcaaaggc ttgccattat
     3001 tctcgggaag gatccgaatt tgtcaatggt ccagctcacc agagagtggg tagatactgc
     3061 tgttaaagcc cttaccccgg cgaaggaagt aaaagacgga cctatcttcg agaatatcct
     3121 tgagggcgat aaaatagata cttttgcctt tccttcacca aaattttatg aacttgatgg
     3181 aggacggtac tttggtacag ccgttttcat ggtcattcag gacccggaga caggagatat
     3241 aaaccttgga acctaccgta tgggaatcct ggacgaaaaa tctgtgggtg tgcaaatcct
     3301 aaagggcaaa aaggcagacc gtatcatgaa aaaatacgca aagcagggta agaagatgcc
     3361 tgcatgcgca atcatcggcg gcgaccccct gcatattttt gccggtgcag caaccattaa
     3421 agcaaaaagt ggatatgatg tagtcagttc tctccgcggt gaaccggttg agatcgtgaa
     3481 aggtcaggtt actggactcc ccattccagc ggcagcagag attgttctgg aaggtgagat
     3541 tgaccccacc cagttgagaa atgaggggcc ttttggtgaa tatacaggct attatactga
     3601 agaactgatc aaacctatcc caaaaccagc actcgatgta aaacgaatat actaccggaa
     3661 caacccaatt ctctgggaaa caagcgttgg caggccggtt ggggatcagc atatactcta
     3721 tgcctttacc cggaatgcaa gtctttggac cgatctcacc aaaatggaaa tcccggggat
     3781 taagtcagtc tatacattgc cagaaggatc cgggcgcttt atcgttgtgg tctcagtaca
     3841 gcagatgtac catggccatg cagaccagat aggtgcagca gtcatagcaa gtaataccgg
     3901 aacgtatgga atcaaaatgg tcatcattgt tgatgatgat atcgatgccg atgaccttcc
     3961 aagagtatgg tgggcgctcg gtacccgtta caacccttta aggggtactc agttgatcaa
     4021 cagaggcaga tcaacacctc tcgacccggc tatcggtgct gatgagaaca aattcataac
     4081 ctctcgcatc atcttagatg caaccatacc tttcgactgg aaaattaagc caacagaaat
     4141 cgctctgaac caggatatgt tcaacaaggt caaggctaga tggaaagaat acggtataga
     4201 ttaaaaggag gaggttcgga atggcatttg aaaaggatat aaacaacgcc cgggaacggg
     4261 cacgaaaggt caagcttttc ggccacgata ttcacggaac tctcacaacg aacagtgtta
     4321 tcgtcgatat cgaaggaaac aggcgatatg gcttctggca tatggatggg tttgcagatc
     4381 tttcactcac tgcaaacgat atcaaggtag cttttcttga taccacttcc atcgatggcg
     4441 aaggactctt cagagcaaag gaactcaaac ttgacaaatt ctattacagc atcaaagaca
     4501 aatccgcaaa aattgaagaa ctgacaaaag agatgggtat atcacttgac gaagtcgggt
     4561 tccttggctg tgaggtttct gatattcctg tcatgcggaa ggtcggtttt ggtgtggcca
     4621 cagcagacgc tagagatgaa gtgaaggctc tcgcagcata tattaccaca aatccaggtg
     4681 gcagaggggc catgagagaa gtgtgcgagt tcatcctccg agccatgggc aaatgggatg
     4741 aatgggtagg aaaagttacc aaaatgggat acaaataggg ggtacacgat ggcaattgag
     4801 aaaccagacg caaaagaaaa agcaaaaaag gtaaaatttg tcattcttga tatccacggg
     4861 atcatgactg ataataccct gttctatact caagaaggga taaaatcgga ggctttttcg
     4921 ctccatgacc gaataggaat caaagcattg acagatgaag gaatcggggt atccttcctg
     4981 acaacaaaaa tatcccgggc tgatgaacag atggccaaaa tctatggact tcccccggaa
     5041 aacctctggg gccagggggc aaaaatggcc agagttgacc agtttgagca ggaaactggc
     5101 tttaaggatg aggaaatctg ctatgcaggc gatgagatga tcgaccttgc agtcatgaaa
     5161 cggagcggat tctctgtagc gcctgcagat ggatcccagg aggcgcgcga tattgccgac
     5221 tatgtgacta ctgcaggtgg tggaaaagga gtcgtcaggg agttggccca gtttattctt
     5281 gaggcgcagg ggaaatggga taattttctt ggagaattta aataactatt aaccggagga
     5341 attatggctt acaagtatta caaagacaac agggcattta tagaggcctg tgtaaaaaca
     5401 ggcgatgctg tagttataaa tgaagaggta gattgggacc ttgaaatggg cgcaatcgtc
     5461 cggagaacat gcgagaagaa aggtccggct gtctttttta aaaatattaa agattatccg
     5521 ggctttgaat gcgttggcgc tcccatagcc acatacagaa ggctcgcaat tgcgtttggt
     5581 ttggatcctg acacgcctat cccagagata tccaaagagt acctgcgcag aactacaggg
     5641 aagtcatacc ctccaaaggt tatcacaaat gcaccgtgcc aggaagttat actccagggc
     5701 gatgatgcaa acattttaaa ccttcctgca cccatggttc atgacggtga tggaggcagg
     5761 tacataggaa catggcagtt tgtggtagcc aaagatctag acacaccttt tatcaactgg
     5821 ggcatgtacc gtcagatggc ctttgatgaa aagaccatgg ctggtccggt gcttcctttc
     5881 tcggatatgg gtaaaatgtg gcacaacaag tatcaaccaa aaaatatacc catgcctttc
     5941 gcagtggtag taaacccgga tcctctgtca gcaatcgcaa gctgtgcacc tgcccagatt
     6001 gcagaagacg agtttacaag ccagctcatg ggagaaccgg tagaaatagt aaaatgtgtc
     6061 acctgtgatc tctatgtccc agcgcatgca gaaattataa ttgagggcgt gcttgtacca
     6121 aatgttacta ttgatgaagc ccctttcggc gagtatacgg gttacagaac cagccctcgc
     6181 gaacccagaa ccgtctacta cgttaaggca ataaccatga gaaaacaacc cattatggct
     6241 atgtccaata tgggagtacc aacagacgaa ggtcagctct taagatcatt ctctcttggc
     6301 cttgaaatgg ataagatgct ccgcgaccag ggtattccca tcaccggtgt acacatgctt
     6361 ccagaatcaa cacaccatct cacagttgtt ggtgtaaagc ctgcgtatcc aaacatagcc
     6421 acacagattg gtcagctcgt attcggcagc aagctgggcc catggttcca tatggtggtg
     6481 gttgtcgatg aaaaaactaa tatttatgac aaggatgaat tgatacatgc tctctgcacc
     6541 aagtgccatc ctgtaaatgg tataagggtg gagccgtttg gtgttggcac cccactgaac
     6601 ccctttgcta caccggccga aagacgtctc ggcgttgccg gcaaggtaat ctttgattgt
     6661 actttccctc ttgattggga agcttcagac attcccatca aggtatcctt cgacaatgta
     6721 taccctaaag atattcagga gaaagttctt gcaaagtgga aaacgtatgg atttaaggaa
     6781 taggaggata ttatggcaga atgggatacc tatattaatg taaatttcaa ggatatgccg
     6841 gaagaaattg aacaggttac tgtgatccgc gatctcaccc cgggcaagta taaataccga
     6901 tccacttacg caaagatcat tgtttccaag gacccggaga aatatccaga aaaggtgtgg
     6961 gtacgcctcg gcagaggtca actgatccct accccatgct ctatgaaaat cctggagttt
     7021 gtgaacatca ttccgaaggg gctttaagtt ggttacctcc ctgaaagagt acctggatat
     7081 tgtgggctca aaaaactggg tcctcaccat aagggaaaag gtttaccttg aagatatccc
     7141 ggcgcttatt gaaaagcttt catgggaagg gaagattctt ctttttgagg atatagaagg
     7201 ttatgattgt aaacttgcag caaaccttgt cccttcccat gatattttca agctgctttt
     7261 tgaaactgaa aacccctatg agtattttat taaaggggtt cagcagagag tcaaacaaat
     7321 ccatgtggcc gtggaaggat tcaaacacat ggaagtatca ggcaagaacc tcttagatgt
     7381 tattcccata ttgaagcatt atgaaaaaga ttccgccccg ttcattacaa cggggatcat
     7441 ttcctcggtt gatccggact ctggaattgt tggaagagga attcaccgca tggaataccg
     7501 cggcgaaaat cttctcggtg ttacccttct caaccctccc ctgacaacaa ttcacgacaa
     7561 atatctatcc agtggaaaga gaatgcctgt cgcaataacc attggagttg acccggccct
     7621 tttcatatcc atggccctta aagcaccttc aggaacagat aagctcgaaa tcgcaggagg
     7681 cttaaaggga aaaggcatcc aggtgtggca atcccctcaa tccaacattg aggttcctgc
     7741 atcatgtgaa ttgcttcttg aaggatacat tgatgaatcc aggagtaaaa aagacggccc
     7801 cctgggtgaa attagcggat attatctgtc ccttgaaaag acacccacga ttgtcgtgca
     7861 acacatgtat tacaaggaaa accttattta tcatgcactg cttcccacaa gccctgaatc
     7921 cgacagatac cttacctttg tgagccgtgc ccatttagag gagagtgcaa aggctctcta
     7981 tccttttatt gtcaacctta cattcgtacc taagacattc gggtcttccc ttgtggtaaa
     8041 tgtcagaaca tcggagaaat tcaagatcag aaaccttata ttattcctcc tgtccttccc
     8101 catgatcaag aaggttgtta ttgtcgatga tgacgtaaat ccagatgatc catacgatgt
     8161 tgagtgggcg ataattaccc ggtgcagggc tgatgaagac ttcattattg ttaacaaact
     8221 ccagggacaa ccgattgatc caactgtaga ggatggctac ggcataacaa aagtaggaat
     8281 taacgcaaca attcaaggta aaatgatcca agaaagggca aaggttatga ggggaaatga
     8341 aaaaagaatc gaggttgttc tgagcaaaat tgacgggagg tgataaatga gcaagatcgt
     8401 ggtagggata tcaggagcaa caggggtaat ctatggaatc cgcatgctca agaccctctt
     8461 tcaacttggc gttgagacac atcttgttgt cacctcatcg gcaattaaga acatgcttat
     8521 agaaacagaa tacaccatgg ccgacctcga accttttgct tccacaatct atgacgtaga
     8581 tgatgttggc gctgctattg ccagcggttc atttcaaata gacggcatgg tcatagcccc
     8641 ctgttctatc aaaacgctct cagcagttgc aaactccttc aactataacc tcctcatacg
     8701 ggctgccgat gtaaacctca aagagagaag gcgagtcgtt cttcttgtca gggaaacccc
     8761 cttgcatgag ggtcatctcg atctgatgat gaaaattacc cggatcggcg gcgtcattat
     8821 gcctcctgca ccggcgttct atcatatgcc aaaaacgatt gatgatatta tcaatcagtc
     8881 tgtaggcaag gtgcttgatc tttttggcat cgacgcctca ctcttcaaac gatggggaag
     8941 caacacaaag gaagagatta agatagtaaa aacgtaaaaa aatagccaat ggaaaagggg
     9001 caaaagatga taggttgcaa aaccgacccc ttggctttca gacctaaacc tttatgtaaa
     9061 cctttaatga attatttatg aaaataacag gtaaaatcgt caaggggctt ggagaatcgg
     9121 caggcttcat agcgatccca tgggtaacca aacagatgag ggaaaaacta aagtttcaac
     9181 cttttggagg aacgctcaat cttgcaattg aagacccaga aatacagcga agtttaaagg
     9241 ctcatggggg tgatcggctg tgcagcgaag ctgcaggctt ttgtgatgcc ctcattttca
     9301 ggggcaaggt cgctggaaaa tatgaatgtg gtattgtggt gcctcttgtg cccaattacg
     9361 acgaacacct tctggaaatc ctcgctcccg ttcacttgaa agactccctc ggccttaaag
     9421 atggagatga agtcactgtt gaactatacc tttaggatga atccatagat cacacctatt
     9481 gcctgcataa gaaatggttg caaaagagcc gctcctcatg gaccctctgc ttcattgaca
     9541 ggtaaaacgt tattcaacgt ggtataattg aaaaagatgg tctgaacgta acaacaaaaa
     9601 cagaggagtg tgcatcatgg gaaaacaagt gtttttgttg tcaggtatac tgttcgtctt
     9661 atgggctggg tttggttttg gtgacgagac atcagcaact gaaaacctga ttcagggagc
     9721 caccctcccc accagggtgg ttggctccat agcagcaatt ggcgatagca taactgctgc
     9781 atttaacgca gcttactcag aattcgaatc ctgcaaattt cgggacactc cagcctacaa
     9841 cttcgcaaca aacaaaagca agaatactac tttcagtatt gctgaacggg ttatggctta
     9901 taagggtgac attgttgcca ctgccaactt tggccaggac ggagcccgca tgtcccacgg
     9961 agatgaccag gcgaaggctg ctaaaacctg gttgatgaag caggccgcgc cacacctcgt
    10021 gatagtcttt ctgggtcaca acgatatctg ttcaggtaca aaagacaagt tttccacgtg
    10081 tgagagtgcc aaccgggacc cgaacaatta ctgccgcacc tctttttttg cctatgaaca
    10141 gcagatgcgt cagatgctcg atgtgctcat cactatcccg gactctcaaa ttctcattat
    10201 ccatcctatc cgggtttccc agctctgcaa cttcaggaat gaaaaagtaa ttgatgtacc
    10261 gtggacttca aaaaaatgtg gtgacctgtg ggatgcaccc atgaacatct tcggtcaaaa
    10321 ggggatatgc ccctccctga cggactgcac agccggtcgc attgctgacg cctacacaac
    10381 ctgggttgcc tatcgtgaaa tcgctgaccg gctcgttgac gagtacaata gctatacagc
    10441 aggcgatacc atcccggcca atgatgactt tgggacaggc agtgttgtca gggcacccga
    10501 tatctctctc gtagcgacca atgttatagg taattcaagg tttcagtaca gagacacgtt
    10561 tggcaacccc cagttgagcg tgtgtgaatg ctttcatccc agtaagtacg gccagaacct
    10621 tctcgccaac cttctctgga atggtgtcac ctgcagtgaa aagactccat gctgtgatga
    10681 ttctattgag agtgatacag attatgatag aggcctttgt aaaagttact ccaccagtgg
    10741 ttctatgaca ggaccctgga ccattcttga tacttacaaa atactaaaag tggaaaagat
    10801 tggttcagga acaggtgtag ttgcgagtac ttcaccaggt atcgattgcg gcagcgcatg
    10861 tgatgcgtcc ttccttcgag ggtcaacagt aaagattaga gcaagggcag atgcagattc
    10921 agtattcaag gggtggtcag gtgcctgtag tggttctgct aaagcctgta cactgggtat
    10981 gagcgaagac cgtgtcgtta cggcaacttt cctaagtgat cctacgatca aggtcacccc
    11041 taccaggaag aacttcaaga aagtgaaact cgccatgaac agagtcatga tttttaccat
    11101 aaggaacaaa ataacaaagg gcaagaaaga tctgtttgtt gggactataa cgtctgacaa
    11161 cccggttttt gtccctgttg atgatctgtg ctcaaatacc tccgtatccc ctaaacgatc
    11221 atgcaagttc aagatccagt tttccccagt tgtcgggcac catactgcaa aaataacaat
    11281 tcccacaaac gaccctcagg caacaaatac tgagattata ctgattggag aaggaatccc
    11341 ttaatagcaa aattgcaccg ctcttacatt gtaaaatata gagcacagtg aagttcgata
    11401 tccgaatcta caaacctcgt agttgatcct ccgagtttgt caccatcatg gggggcacag
    11461 cagtaccacc cctcccctct tcaactgggc aaacacagtc accagacggc tcaggaatgg
    11521 taagttgtat gctggcacag gaattgcttt tgattattcc agtcagatgt gacagaaaag
    11581 aatatctcag gcttgtgagg aaggtgtaag aaaagcttta aacataacag atcttcttga
    11641 gatttgtacg acgggaggag gaatactttg tttcttcatg tacagtatcg agattaccga
    11701 cacgactttg ttcaggcccg tgatcttgat aggctcatag agctgaggca aatcaaacag
    11761 ttttatcggc cctcggaggg tagatgggtt gatatagcaa ctgatcgggt tcgaggctcc
    11821 ggaggttatt atgggggacc cgagagaagg cgcagccaac cgggggcaat gtgactgtat
    11881 agcacgaagt tgcatgtatc acagcactta tgaaacatac caggcattaa cagtcttact
    11941 cgggtaactc agctggaacc acaagtgagc ccgcgcatat ttcctgtttt gcatggtctg
    12001 ccttcaggca tacacactca tccttttcga attggactat atcacaacac catatgaaac
    12061 aatcctaaag gctcattgag cttgattttc catctatttt tcagtatatt taaatgactg
    12121 agctctgcta catggcagcg agatctgaga atgaataccg aacaatattt caaaaaaaag
    12181 gagtcagaca tatgtcagaa ataatactaa agaaaggcaa attgtcatcg actctattga
    12241 gaggcttggt cgattattat gccgcaatac ccggtgtaga caaatcttac gtcactttga
    12301 ggtgggacca gtggaaacaa ggggacaata ttgcggtata tcttctcaag aaggatgaag
    12361 agcctgttgg ttggatcata tacaatcgag ccaccagcac catcgaagaa atattgttga
    12421 atccggataa agaccaggcg ctggttcgct ttcaggctat tgatgcgctt attgataggg
    12481 aaagtctatt atctgcggag attcttgaag aggatacgga gaagttttac tggctgggag
    12541 agtacggttt ccgcccgacc cggaaaatac aggtttttgg ccagactctg attaagatgg
    12601 aattaagcac agtagtcttt ttccagcatc tcaaagaaca caaacccgcc aaaccttatc
    12661 gtaagaaaga gaaggtcgtc atcgaacagg tgccatctcc ccagtcagaa tcagaaataa
    12721 aggcttcact tcaggatcta ttgaacaagc tcgggggaat aaaaaaatac gtaaaaccgg
    12781 gacagaccgt tgtgattaag ccaaatgtgg tagccgacca cggtatgctg ggcggaaaat
    12841 atactggtgg tgtagttact gatattcgtg ttctgaaagg ccttatcgag ttgttactcc
    12901 cagttgccgg gaaagttatt gttgctgaag gctcgtcaat taatagaagt gccacagtta
    12961 agatgtttga aatctacgga tacccgaagc tgattgacct tgatccaaag aaggtaagcc
    13021 tcgtcgatct gaatactgac caattggtag aaaagctggt tccagccggc aagaggatga
    13081 agtcccggaa ggtgccccga acgattgaag aagcagacat cgttataagc gtgccagtaa
    13141 tgaagataca ttttgctgca ggtgtctctc tcggagttaa aaacttgcaa ggagccatgc
    13201 ccccccttga gaaatacatg actcactttt tcggtctatg gcagaacctt gtcaatatac
    13261 atcacgttgt caaaccgaaa ctgactatta tcgacggtat cgtgggtcag gaagatttcg
    13321 ggcctgtatc aggtacacca aagacaatga accttcttat aggcggagaa aaccctgtgg
    13381 cagtcgatgc cgtcaccatg agggtcatgg gcctcgatcc tcatatttct cctcctgtgc
    13441 ttctagcata catgcaggga ttcggtccta ttgaacctga aaatatcgag gtgctcggca
    13501 caccaatcga taaggtggca aaacccttca aacaaccttt tctcaatctg gaaagcggaa
    13561 aatatttcaa agtccatgga actgatgcat gcaccggatg caggggatac cttcactttg
    13621 cccttaataa gttgagaaga ccagacccgg cagacccgag cagattgctt attgaccgac
    13681 cctttgaacc gaaggtaaat atttatttag gtccgtatga aggtgcaaat gccgatccga
    13741 aagaaacaaa cattttcatg ggaatctgtc agctccatca cacggaaaat ggcatgtctc
    13801 ttgtgggttg cccaccccat gcagaagtca ttatgaatgg tatttttagc cttttcccag
    13861 acgtggagag accaaaatat gctgacgaca cagaggaggc aaagctggaa cgaatgctca
    13921 aggaagcgct ggctaccctt gcgtaagaaa aactggtgca ctaaataacc aacctcgttc
    13981 cggggtatca taaaaacacc tgaacaaaat taaaaagccg ctgtcatttt agagcagcgg
    14041 ctttttaatt tgtcggcatc tctttttgtt aatgttctac tcgattcctc acgtagcaca
    14101 cacacctgat ggaaagcagt cagaagctat ttctacctcg gtggtagact cgactacttc
    14161 ctgggcaacc ttagatatcc tgcagagtag cgccttcatt tctgtgtatc ctgtcactgg
    14221 atctctcggt tcaagttcgg ttaacacatt tgcattggcc ttatcccaac cgtggggaac
    14281 gctcagtacc ccacgaagca gcttatccgt agtctgtgcc ttaataaata ttttcccctt
    14341 cggtgtttct acgctcatca tatcaccatc tgcaatacca tactgagccg cggtatcagg
    14401 gtggatctcg gccagcggct caggtgcagt cctatgcacg tatggtacgt ctctcaactg
    14461 ggtatgggta tactcgggaa tccgtgctcc tgttgtcagg atgaccgggt actttttgta
    14521 catctccggg ttgctaacag ggcttttgct tggctccaca ggctttggca gtggatcata
    14581 cccgttctct gccagtgtct gggaataaag ctcgatcttt cccgaggggg tgttatatct
    14641 tttctgctgc acatatttca tttctgtata gtaggcgccc tctgggtgtt cctttgtcag
    14701 ctgttccctg ttcaatccac ttggtttcag gaaaaagtct ataatctctt cttccgtatt
    14761 ccagggaaaa aactctccat agcccatcct tcttcctatt tcagtccata tcttccaatc
    14821 tggccagcac tcaccgattg gctctatcac tttctttcgc accatagcat agggaataca
    14881 gttggtcaca ccgtacacat aacctacacc tgctttctcc agaaaagaac acgccggcag
    14941 cacaatatcg gcaagcttag cagtctctgt catgaacagt tcagatacca caacaaagtc
    15001 aagggccttg attgcttctt taatacgctc tgaatcaggt aacgtaactg ctggattgcc
    15061 gcctgtacag atcatgcctt taatgtgata tggtttgcct ttcaatacgg catcggcaaa
    15121 tagcatctgc tgaccgtaag gagatgttct tccccagaaa cgcctgaaaa gaggatgctc
    15181 atcagtgccg atggggtcac taatctcagt aaccctcatg tcgccaaggc ggataaaagg
    15241 aatgctcacc cagccaccgg gaacatcaat gttgccagtt atcgcctgca tgatagcgtg
    15301 aacgcggtta ccctggaacc cattaacatg ctggtctatc gagcatgttc cgggaacgat
    15361 tgaagccggt ttaacggttg caaacaacaa tgcgattctt ctgatgtctt ctgcaggaac
    15421 ccatgtgatc tcagacattt tttctggtgt gtactgtttc acatgctcgg atagtttgtc
    15481 aaagcctatg caaaacttct ccacaaactc tttgtcatag agaccctgct caataattac
    15541 gttgagcatg ccgagacaaa gggctgtgtc ggtaccgggt ctgatctgga tgtgaatccc
    15601 tctttcagcc atagggatct tcttcgggtc aacaatgatt acgtgcttga gggtgccggc
    15661 atccatggcc ttataaatct tgcggcccac ggttatcctg gaattgtcca tattctggcc
    15721 aaaaactata atacacttgg agttccaggg ttcctcaata ggatacccgc caaaagtcat
    15781 ctggcgcgcc ataatacgtg atcggaaaca gttcccttcc actgacaaca ggttaggtgt
    15841 cccataaact ccccgaaacc tctgtgcaaa ggctgccagt tctatgttct cggttcccag
    15901 tgagccagta tatacagcaa gagccctggc gccatacttg tccttaatct cctgtaactt
    15961 ctcagctata gtatcaagtg cataatccca ggaaatgcgc tcccaattgc catcagtctt
    16021 cttttgcatg ggaaatgtaa gcctgtcctt tgaatacacc cactccggca gagcagttcc
    16081 tcttgggcat atttccccca aactgatggg gtgctctttc atgccttcca cttttacaag
    16141 tttgccgtct tcaacatacg cgtttattcc acagctattg atgcacatta tgcagtccga
    16201 tttaatcact tgcattgcca ttgcactgtt cctccttata taatatttct tccctctacc
    16261 ctttcggaag attttattcc attaaaaatc aggtcacata cctcctcata tccttcaatc
    16321 agatcgtatg tttcctcctt gagcagccaa cgggttaccc tttgttccag gaccccgagt
    16381 attaactccc gtatggtata tgcacttgta tcctttctaa acagaccact ttcctggccc
    16441 tctttaatga tttcaagaac cttttcagtg aatgccttta tcttgccata tgtttccgat
    16501 tttatgaaac ccctgcttac acgcatttca agcatcaggc tccgggcata cgctgggttc
    16561 gatttaaaaa aatagagata ataccagatc aattttctga tttttccaac accatcatga
    16621 attccctgga gatggagatt aaactcttcg cagaactctt ctgttttttg ggcgggaata
    16681 gaaaagaaaa ggtcttcctt atttttgaaa tattgataga ctgtaccctc agcaatatct
    16741 gccttctggg caatctcagc gatgctcgca ttttgaaaat tactgttccc aaaaatttca
    16801 gccgctgccc tgataatttg ctgtctcctc atatcctttt ttgacatctt gaatgcctgt
    16861 aagctacaat tgaactatat tcattatatg acatttattc atgtttgtaa acaacaaaat
    16921 tatcctctaa cttaccacct attaagcaat tatataaaat tgaattatat tcaattatac
    16981 gaatgctcac atcaacagtt atgtgaatca gatttatcaa aatgactata gacgctttaa
    17041 tacattttgc acatatggtt aagatgaacg tcaaaatcat atacatgttc agaatggggt
    17101 aggaaaaaga aacgttcata agccggagtc aaggctcaat cccgaaaata ttgtgttcag
    17161 aatgttgtag ccacggataa taccagaaac atcaaggatt acatgcataa aagccttttc
    17221 gagagtaacc acccatgaag gctgaccatg gcctgttcgt accggaagtc ttttccgcct
    17281 ccgggttaac tgttcttgaa gcccacaagt ccctgggtca atacataggc tgcctgcaaa
    17341 tacgatgtag gacaaagagg aaaacactct ttgcagttcc agcactcctt ggaagcaatc
    17401 tcaggaataa aatttatctc ccgttttggg ccctgatcga caaaagcaat ggcattcttc
    17461 ttcttaactt ctgcgcaata tctaacacat aacccgcaaa gcacgcaaaa agacggctct
    17521 ttcccaaagc gtacactttt ggccccatat tccttggcca tctccagcaa ctgaggagaa
    17581 tcaggtgcat gtgccagtaa cagctctatg agcacctttc gaatcttatc tattttctca
    17641 gacctggttc tcacaaccag gtttttttcc acaggataaa gacaggaagc cacatagttt
    17701 gtccgtcccc gcgactccac ttccacagta caaatccggc agccaccata aggttccaat
    17761 ttttcgtgat gacacagtgt aggaatgaat atgccactgc tctgcgccgc attcaagact
    17821 gtcattcctt ctttagcccg tacttctttt ccgtcaatcg ttaaaaggat atcgctcatc
    17881 ttttttactc tctttggcta tttttcttgc taacttgcca tggtagtcat acacatccct
    17941 tatcctcaat gcttgcctcg tactcatttc tgaaataacg cagggagcta taaatagggc
    18001 tcgatgtcgt tttacccaga gcgcacagtg aggcgagaga gccaacttcg caaagctctt
    18061 ccagggcttc tatgtcaccc tctttgccct ttcccatact catccgggtc aaaatacgca
    18121 acatctgcct tatcccttcc cggcatggaa cacacttgcc gcaagattca tctgccagga
    18181 actcaaggaa gaacctgttc atttcaacca tgcttgtatc ttcatccata acgaacaacc
    18241 ctccggcccc gatagccgca cccagttttg acatgacatc aaaatccaaa ggggtgtcaa
    18301 taagctctgc aggtaatgat cctcccatag ctgctccgaa atggacagct ttgagcttct
    18361 tcccatttgg gattcctcca cctatatcgt agacaatctc tttcagagat gtgccgaagg
    18421 gcacttctac aaccccgcta ttcactatat tgcccgaaag ggaaatcatc ttggtcccct
    18481 tgcttccctc actccctatt cccaaatacc agtcagctcc tttgccgata atctgcggca
    18541 catttgccca cgtctcaacg ttattcaaat tgcttggtcg ttcccagata cctttcactg
    18601 cagttcttgt atatttcggc cgtggctcag gagctttacc ctctattgat ctcatgagtg
    18661 cactcgcttc accagacacg aagattccca catcaagatg cacttcgata tcgaagtcaa
    18721 atccagatcc gagtatatcg ttgccaataa aaccatgttc tcgtgcagcc tcgagggcaa
    18781 tatcgatgtt ctctctcaat ctcggcatat catgacgagt ataaataaaa ccctgctttg
    18841 ccccaatcgc atagcctcca attattaaac cttcaatgat caaatggggg tttccctgaa
    18901 aaatggcacg gtccatatag gcgcctggtt caccttcgtg accattacat atgacatact
    18961 tcaattcagc aggcgcatta cgtgctgtct cccattttaa gcctgccggg aatccaccgc
    19021 caccccttcc ccgcagattc gctttcttca cctcttcaag aacttcctgc ggtttcatct
    19081 taaggagggc cttcgccagt gacgaataac caccgatggc cagatagtca tcaatgcttt
    19141 tggggtcgat tttcgggttc atcccaagga tgtttcgtac ctgcttttta taaaagggta
    19201 tttcagactg gtggactgct ttctcaccgg tattcgggtc tatgtacacc aggcgatcaa
    19261 ctatcgtctt cccgaccaca gtttcagaaa tgatttgctg tgcatcctct ggttttactt
    19321 ttgtataaca gatctcttca ggatatataa caatatttgg cccttgggcg cagaaaccat
    19381 gacagcctgt cacgcgtaca tcaaccttgc cttccaactt ttgcttctta atctctttct
    19441 caaaggcccg gataagatcc tcacgcccac caagacagcc tattccaccg catatcgcta
    19501 tacacggctt attcggatct tttttcaaca caatatcttt tcttaccttt tccaactgag
    19561 cagcagaatt tattctcgcc atagcattcc cttatttata gtttttcaag atggcttcca
    19621 catcatccgg cgtaatgttg ctgtgatgca tgccgtcaac cacaatctcg ggccccaatg
    19681 tgcagcatcc aaggcagctt cccttttcca gactaaacct caggtcaggg gttgtttctc
    19741 ccggcttaat accaatcaaa tcttgaactt tttcaaggat ttttgccgaa ccgcgctgat
    19801 gacatgatgt gccggtacac acgtgaacct catgacgccc ctggggcttc aggctgaacg
    19861 tcttataaaa cgtcgctatg tgcagcactt cacttaaagg cacacctaat ttattgctga
    19921 ccttttccaa aacctcccgc ggaagccatt tgttttcatg ctgaatatcc atcaacacca
    19981 taatgaccga attaggctta ccgtcatatt tattaataat ctcatcgatt cttctattgt
    20041 ccatgggtcc tgcgttcctc catttcgtcc tatgcccgag cctggtcttc tctcatctgc
    20101 gggaggacaa ctctctggct ctcatcaaat ctgagcaaac cctgtcttgc agacaaattc
    20161 agatgcttgg agacttcaga gggttttaag cccagaatct cggatatttc ttcgcctgag
    20221 cggggtcctt ctcgcagaag caacataatt tcactcactg caagtttatc tgttattaat
    20281 tctttaaaaa gctcatcaaa ctcttcactg gaataaaatt tccggtattc ttcctctgag
    20341 tttagattaa ttctcatcct ttctctttcc acaagcctca tataaggaac tatgttattt
    20401 acagcttcaa acttaaaggc tagtttcttt ggttcaatcc cttctgcctg tcctataggc
    20461 ccaagctctt taatcttctt ggagacgtca ttcataattt cagcaaatcg aacaccctca
    20521 gacgccccca cccattctat tcttaacctt tcagggttta accctatatg agtcaatatt
    20581 ctacgcataa ggaggacatt gcttaaggca tggtaattac cttctgtcac atagtgacat
    20641 tcaccgggcc agcacccccc tatgtagact ccatctgcgc cgttcgagaa agcgcggaca
    20701 ataaacgccg ggtctactct gcctgtgcac attacacgga taagcctgat atctgttgcg
    20761 tattgcagtc tggaaactcc agccaggtca gcagctccgt aacctcacca gaagcagata
    20821 aagcctaata ttttcggttt aaattcgaat ccattactca tttttatctc ctacctgttt
    20881 atcacctcaa tagatattac cgtgcccttc cattcatctg gatagcctca tcaatctgtt
    20941 gaaaaatttc ttcgtccgta aagtgcttga gtgaaatcgc tctcgtgggg catacgggat
    21001 tacaaagtcc gtctcctttg caaaggacag ggttgacata ggccttcttc cccttcggcg
    21061 tatcacgaaa ttctatagcg ccatatttac acactgatat acaggctccg cacgatacac
    21121 aatcgttttc attcacttca cagacagcac cggaggccac taccgtatct tttgagagta
    21181 ttgtgacagc ccgacctgct gctccatagg cctggctaat tgcttcagga agatgcttgg
    21241 gataatgcgc tgcaccgcat agaaatacac catcggcagc aaagtcgacg ggtctcagct
    21301 tcacgtgagc ttcctggaaa aatccatcag gatttaatgc aaccttatag aaccgtgcta
    21361 tttcagccgt tgttgatgaa gggattgcag cagcagccag ggcaacaata tcagcatcca
    21421 attccagttt cttgtcaagt atataatcta tggtggtaac tttgagaacc ggcttgcctt
    21481 cttccgactt acccggctct acgtgtggcg gatcctgagg ttcataacga atgaacctca
    21541 cgtctttttc agcggcttcc cggtagtaat cttccttgaa tccataagtt ctcatgtcac
    21601 gaaagaggat gtagatatcc atgtctggat tcttttcttt cagcttcaga gcatttttta
    21661 tagattggct gcaacagata cgggcgcaat aatttctgtc ttcatttcta cagccgacgc
    21721 actgaatcat tacaagattc tttgcattca ttacattttc atcttcattg acaatcttcc
    21781 cttccaactc caggttcgtc ataaccctgt cgtcctgtcc ataatggtat tcgacaggtt
    21841 tatattcttc ggacccggtg gctataaccg ctactccgtg gcgtatttct ttggccatac
    21901 taccggatgt tatcctggtt gtaaaatttc ccacatatcc gtgaacatca tggatgatgg
    21961 catcggtaaa gacgtgaagg agcgggttgc gatacaccct cgatatgagg tcactcagat
    22021 atgcctgaac gtccagtcct tccagtgtgt aataaatcct tcgggccatt cctcccagtt
    22081 ctggttcttt ctcaaggagg aaaacctcaa aaccctgttc ggcgaggctc agtgcgctgg
    22141 tcatgcctgc cacacccccg cctataacca gggccctttt gtcaaccggc agctcgaatt
    22201 cttcaagagg ttccaggagg gctgatcttg ccacggacat gcggacaata tcctttgcct
    22261 tttgtgtggc aatctctttt tcttttgagt ggacccagga acaatgctcc ctgatattgg
    22321 ccatttcata atagtactgg tttatgcctc cctcgcgaag agtatcccgg aaaagtggtt
    22381 catgcgtcct tggagtgcat gctgcaacaa ccacgcgatt aagcccctta ttcttgatcg
    22441 tttccgatat ttgttttgcc gcatcagtgg aacatgcaaa cagggcttcc tgagcgtatg
    22501 tgacgttagg cagattcttc gcatagtcaa ccacagccgg tacatctaca actcttccaa
    22561 tgtttgctcc acagtgacaa acaaataccc ctatcttagg ttcttctttc gatgcatctc
    22621 gttcctgggg atattctctt tcctttgtca gctcgttccg gcgatagtca agaagttgac
    22681 cgcactggga gcttgcaccg ctggccgtca taaccgattc tggaatatcc acaggcccct
    22741 gaaaagcacc acttatgaaa atacccggcc gggaggtttg aatgggattg acaggactga
    22801 ccctgcagaa gccatgctcg tttaactcga ggccaaattt agtggccata aaggctgcat
    22861 cttttggcgg gttcatgccc acagacagaa tcaccatatc aaattcttct tctttcacac
    22921 ccgcgtccgg ggtggagtac cgcaccgtca cattcttcgt taccggatct tctttcccta
    22981 tagagacgta gctccgtata aagcgaattc cgggaagctt ctctgcccgc tgataaaatc
    23041 gttcaaaatc ctttccaaat gctctgatat cattatggaa tatagtacac tctgcttctg
    23101 cgtcatggtc tttggtaaga atgacctgct tctgggtata tgcacagcat actgcagagc
    23161 agtaactgtt gccgccttca ataacctgtc tgctcccgac gcactggatc caggccagct
    23221 tgtggggatg cttcttgtcc gatgacctca gtatctcacc ttcgtatggt cctgttgcgc
    23281 agagtatccg ttcataatcg aggcttgtca ctatattttc aaattttccg taaccatatg
    23341 tatctctcag ggttgccgga tcaaatgcat cgtaacccgg cgccaaaata attgcgccaa
    23401 cctctatctc cagtgactca ggtttttgaa aaaggtctat ggcattattc ttacagaccc
    23461 cctgacagat ggtacatctc tcatcttgta aaaaggagca tttgtcgcga tcaacatacg
    23521 tgaccagtgg aacagcctga gaaaaataga tatggatagc tttattttta gacaatccct
    23581 ggttgtattc atcctcgacc tgaacagggc agtattccac acacacaccg caacctgtac
    23641 atttgtcttc atctatatat cttggtttct taacaagggc taccttaaaa tctcctgctt
    23701 ctcctgtcac cttctctatt tcagtatacg tcagtatctc tatgttgggg tgcctgctgc
    23761 attccacaaa tttcggagat tcaatgcaca tggagcagtc attggtggga aaggtcttgt
    23821 cgagctgggc catatggccg c
//
LOCUS       DAOOMC010000028        23712 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdus sp. isolate AMR_MDS_2685
            NODE_2026_length_23712_cov_6.225937, whole genome shotgun sequence.
ACCESSION   DAOOMC010000028 DAOOMC010000000
VERSION     DAOOMC010000028.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864760
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdus sp. (wastewater metagenome)
  ORGANISM  Syntrophorhabdus sp.
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae; Syntrophorhabdus.
REFERENCE   1  (bases 1 to 23712)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23712)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,729
            Genes (coding)                    :: 2,708
            CDSs (with protein)               :: 2,708
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 7 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23712
                     /organism="Syntrophorhabdus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2026_length_23712_cov_6.225937"
                     /isolate="AMR_MDS_2685"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2699756"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(369..1871)
                     /locus_tag="PK125_04370"
     CDS             complement(369..1871)
                     /locus_tag="PK125_04370"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HPB37376.1"
                     /translation="MNVAANLETSTLYFPKSPALVTGEKEMTYAEFNDRTNRLATGLL
                     KMGLRPGEHVAICASNSEEWPISYFAILKAGGVVISLASLLSSDELRMLLEHSKPRFL
                     FTNSERLKDIDGLRGNQGIEKVICPDGDITFDHVISSGLSSFRSLERDRTDTAVILYT
                     GGTTGMPKGVMLTHENINHPAHNVAFCERTTQGDRALCALPLHHVFAVVHIMTSTIMS
                     GACLEILPGFDMETVLSVIEAGQVTKFFAVPTVYTRFVTLDHLEHIMRNIRYCFCSGS
                     AIAVEIMRQWKERTGLNMHEGYGMTETASAVTFNHMYRHVPGSVGTVVPGCEVQIRDA
                     AGNILETGKEGEVCMRGPNMMKGYLNNPEATEEAFWEKGWLRSGDIGVLDEDGYLTIV
                     DRLKDLIITGGENVYPREVEEAIYLNEGVAECAVIGAPDKVWGERVVAYIIPKVDVSI
                     TADGLKAFLKTRLAPFKVPKEYIFTTEFPKSTAGKVLKREIKRQHLEKNK"
     gene            complement(2014..3201)
                     /locus_tag="PK125_04375"
     CDS             complement(2014..3201)
                     /locus_tag="PK125_04375"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPB37377.1"
                     /translation="MQRELWSKEFIAIIASSLFNSLAFYALMPTLPIYLLEHLKMSHS
                     SVGLVIAAFSISVILIRPVAGYIMDNYHRPGVFVLSLSLITALYGVYPLISTVAGMFT
                     IRFIHGTFFGITSSASATIVADIVPPSRIGQGIGVFALSFPVGMTIGPMFGLELIKNQ
                     GPNTLFLAITVISFLSVLGAFCTRYPYKSPARKKFSLPDLFSKMALPISLCMFFIMAV
                     YGAIIIFVSIYASQKNFPNVATFFVVLSVAVFLSRLFAGRLFDTGHALHLIVAGLIMA
                     STGVLWLGYAGNSTQFLVAGVINGFGFGILMPTGQAAINNLVKSSERGAANSTYLFSY
                     DLGIGAISLIIGFLLDKVSLAEIYRYSTLLIMLAAIIFVFKAIPHYYHNIRDRKITPH
                     FKS"
     gene            3369..4148
                     /locus_tag="PK125_04380"
     CDS             3369..4148
                     /locus_tag="PK125_04380"
                     /EC_number="2.3.1.286"
                     /inference="COORDINATES: protein motif:HMM:NF001753.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HPB37378.1"
                     /translation="MNDQIENIAELIVNAKRIVVFVGAGLSTESGIPDFRSPGGVWDK
                     YDPEDFYFQNFVSSEASREKYWQMATEMYDSMKDAMPNQAHLAVAELEKLGKLDCLIT
                     QNIDGLHFKAGNTPDKVLELHGTAMYVTCLNCNKRYDREEVQLRIAKGEKAPRCDECT
                     GPLKPATISFGQSMPEQETREAYERSGTCDLFIVIGSSLVVQPAAYMPVVAHKSGAKL
                     VIINRDETSCDDIADIVVKAQAGPTMASVLARVREALSKKQ"
     gene            complement(4162..5592)
                     /locus_tag="PK125_04385"
     CDS             complement(4162..5592)
                     /locus_tag="PK125_04385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB37379.1"
                     /translation="MKVKNSALLSLITGAMEICWIYSWVSFTMTAVLGNTISLLETAA
                     AFSFAIVLTNVSTGHGWRVVTLIITHILGCSLAVAYVLHGIFYPSYPIFNTHWISLFF
                     TVAREPMDWVHHIFILICVGLTWLAGTAFAKRRKTYSVICARFDIGLAAFFILLLAKL
                     TLRVKGGINVDDSLSSALIYPFLLLSIIAIGITRVGHGGVRRFLPGRGGSGILMSTVS
                     VVILIVSTSVFLLLPVLTQVADTGQRVLKGLALWILPVVSSVVRFMFMGGQVRSDPSS
                     GSPPGKDFGSESLFADSWWMELLEVILRKGIEIIAIMFFAFTIALLIYLIMRWLFSRT
                     AINSETARNKNHFLPWYKRLWQFLHSLWKAVLGVAQGYKRASELFSVLTEWGRRSGLP
                     HLMTDTPFEFGIKLCGQFPKLKMEIETIINALTIEIYGKEGLKSEQLASAVNAWYTLR
                     SPVYWPRRLKMRIISKIHSRQGIHNL"
     gene            complement(5589..6734)
                     /locus_tag="PK125_04390"
     CDS             complement(5589..6734)
                     /locus_tag="PK125_04390"
                     /inference="COORDINATES: protein motif:HMM:NF013998.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HPB37380.1"
                     /translation="MGIFLFIGLLNRQHDLSILCILVFGIAIILRVWAKMAGKKIVCR
                     LISDRTRIFPEENITFTLTVENHKPLPVWLDIEMPVHASTNAVTETGTFYGQQTLLWY
                     QQALFEWQTATLKRGVYEVGPLQVLSGDLLGFFQFESTDRETAEIVVYPKIVPLPLFS
                     LPKREFFGAPGGESPVDDPVYILGTSDYHYGRPARYIHWKASARHQRLQEKVFDSTVQ
                     EKILFLINIEGFTKAENKESFEQGLEIIASLALQFDRRGCAMGLLTNGEVKGSSSSVA
                     VSRGSQQLARILEALARLQPVQNNVFIDILETTGAVPWGTTCLCLSLNNDDSTKIIVE
                     YLKQRKIPVIMVPWEMDSLLRKNNPVAPEIHAPLINGLIGVEEVQGI"
     gene            complement(6807..7745)
                     /locus_tag="PK125_04395"
     CDS             complement(6807..7745)
                     /locus_tag="PK125_04395"
                     /inference="COORDINATES: protein motif:HMM:NF040105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HPB37381.1"
                     /translation="MTFDLCKQITGNMSSVIVGKEQAIELLLVGLIAEGHILIEDIPG
                     LGKTLIAKSLAKSIGASFKRVQFTPDLLPADITGFNVFDQRSGQFVFQQGPVMTNVLL
                     ADEINRTIPRTQSSLLESMEERQVTVDGKTHSLPHPFFVMATQNPIELEGTFPLPEAQ
                     LDRFLLRIRLGYPDQDEEIAILQRFQEEDPLQTLSAVATPADISRLQKARRKIHVSGA
                     VKEYITSIVGATRINPLLRLGSSPRGSLGLMRSGQALAALRGREYVLPDDIKSLAVPV
                     LSHRMILREEERLRNTSQEDIVNQILNKISVPVSEK"
     gene            complement(7805..8290)
                     /locus_tag="PK125_04400"
     CDS             complement(7805..8290)
                     /locus_tag="PK125_04400"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HPB37382.1"
                     /translation="MKGLLPGYKKCFFCGPATGGLGLELKYMDSTASCEFTAQERFQG
                     YHGMLHGGIVTGILDEVMWWALFMKTKKICATWKMEAEFKRPIMCGKTYRASGQFVSS
                     THNMYYLSAMIEDETGKLCAQGKASFRKTRGFTMNEIIEQLDFRGVPCEIRSIFQSLE
                     M"
     gene            complement(8414..9256)
                     /locus_tag="PK125_04405"
     CDS             complement(8414..9256)
                     /locus_tag="PK125_04405"
                     /inference="COORDINATES: protein motif:HMM:NF024798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase"
                     /protein_id="HPB37383.1"
                     /translation="MEYKNAHPLGDKKITTINTCFIITVIGLLFMFSSPVTGEIDPQY
                     KDRIDTLTAKILVTGFSEEEIRQIFSDERVMIYPQILERSGKGLPYFSKKFGLLTKKS
                     LERGQKFLNENKEVLVRVEANYGVDKEIIIAILRLETNFNRYVGKYPIFNSLLTLALV
                     ENRRSSWAENELIELFWIAKMLNKNPLSIKGSWAGAFGMAQFIPSSYRKYAVDENNDG
                     IIDLFDVPDACASIANYLLSHGWEKNNPDKNRKAVYAYNHCDEYVKAIFAYAKALKSG
                     SAKK"
     gene            9408..9851
                     /locus_tag="PK125_04410"
     CDS             9408..9851
                     /locus_tag="PK125_04410"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF024970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPB37384.1"
                     /translation="MKKSLDEKEPDKIVRPAIPDSVQDLIEFVSSIVFETGMEDDKVK
                     TIGLAIEEVVQNIIEFACPEGKEEITISCELLDSGATLIHIWDTGKPFNMLLAGTFPE
                     VELVSAHEKIPSTKMLKKAVKNIEYIRGKDKNILILTIPPVRGRN"
     gene            complement(9967..11403)
                     /locus_tag="PK125_04415"
     CDS             complement(9967..11403)
                     /locus_tag="PK125_04415"
                     /inference="COORDINATES: protein motif:HMM:NF023222.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-hydroxylase N-terminal
                     domain-containing protein"
                     /protein_id="HPB37385.1"
                     /translation="MGIKTKEEYIASIGALKPTAYMFGKKLTSIVDNPRLRAGIEATG
                     ATYELANMPENKDLMITISPLINEPVSRFTLPPASIEDLVARVKVNRKVANYVGTCHQ
                     RCTGLDCLSTLAIVTYDIDKKYGTNYNKNFTEFLKHMQKNDLTANAGVTDVKGDRSLA
                     PHEQVDKDMFVRVVEKRDDGIVVRGAKAHQTGSLSSHEIIVIPTRAMGKNDKDYSVSF
                     AVPSDTKGLIHVVGRSSLDMRELDGCDIGNAYYSKYCPTLIFDNVFVPWERVFMCGET
                     EFASEMVIKFSSFHRQSHGGCKSGKIDCMVGAALTLMDYNGTSKAGHLKQKVIDMIHR
                     AETLYGCCLASSYEGKQQPSGAYFIDTVLANASKIHEGKEMAEATRLMIDACGGFVAD
                     LPSDRDFENPDIGDLLKKYLKAVDTVPVENRVKMYRLAEKLALESADTISDIHGGGSP
                     EAHRVTIFRETDVESKKKAARRLAGIKE"
     gene            complement(11452..12150)
                     /locus_tag="PK125_04420"
     CDS             complement(11452..12150)
                     /locus_tag="PK125_04420"
                     /inference="COORDINATES: protein motif:HMM:NF019349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HPB37386.1"
                     /translation="MKPKTKKTTKPQADSSQIAYQGIRRMLYTKELAPGQKIAYRDLA
                     EKLKLSPTPIIQALKWLELQGFVLHEPKRGYSMAPFSLKEIEELYELRELIEPSLIPA
                     TIQHINRDGLKNLKTALEAHFSAEREFYLKERLFKNKEFHMTLASLSEKETQIRILQN
                     VFDMLFLKYGGNYLPMSSLTATDQEHQEIYDAVALRNLERAQNVLKNHLTNVKIQVLS
                     SVKKILAEQERSPF"
     gene            12516..13655
                     /locus_tag="PK125_04425"
     CDS             12516..13655
                     /locus_tag="PK125_04425"
                     /inference="COORDINATES: protein motif:HMM:NF024938.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HPB37387.1"
                     /translation="MRLHEYETLDIFEQNRIPVPRRGIAGTTHEALQIAGEIGFPVML
                     KAQVLAGGRGLAGGVRVALTQDELEDVAEEMLSSDIKGLPVRKVLVCEKAAIEKELYL
                     GITVDGYSGRPVIVVSTEGGMLIEETARNSPEKVASIHIDPSFGYYPYLARSLLRRLG
                     LKEQLIASWSDIIGQLYNIARRYEALIAEINPLVVRPNGGLLAVDAVLEVDDSALSRI
                     RFPLPDRIDRIENPLERRGREIGVTYVDLDGDIGLISSGAGLGMASMDIIGEKMKPAN
                     FLETGGGITADLLYKCMELIMMKPDLRAIFINVYGGINPIHEGAKGVVRYIHERNVRI
                     PVVAKALGNRQEETWEIFRSAGVHVVTETATEKAIDKLYELVGRG"
     gene            13659..14531
                     /gene="sucD"
                     /locus_tag="PK125_04430"
     CDS             13659..14531
                     /gene="sucD"
                     /locus_tag="PK125_04430"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018287377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HPB37388.1"
                     /translation="MSILIDDSTRVIVQGITGRIGSVQTHWMLEYGTRVVGGVTPGKG
                     GTTVEGVPVFDSVLEAVKETGANASVFFVPAAFVLDAFLETIDAGIKLIVVVPEHIPV
                     RDVMKMRSYAVEKGVFALGPTTPGILSPGKGKMGIMPASLFAPGRVGIISRSGTLSYE
                     FAGILSEVNVGQSTVVGMGADPVVLRNLADILELFERDTDTDAVIIVGEVGGEQEEKG
                     AGFISRRMTKPVAAYIAGRQSPQGKRMGHAGAIVRGAAGTVEGKQKELRAAGVEILES
                     PIHVADWARKNNLR"
     gene            14737..16083
                     /locus_tag="PK125_04435"
     CDS             14737..16083
                     /locus_tag="PK125_04435"
                     /inference="COORDINATES: protein motif:HMM:NF015882.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent malic enzyme"
                     /protein_id="HPB37389.1"
                     /translation="MEKNKVTKEELLEKAKKPAQDALKMHPYYKGKIEVVPKCVIRDI
                     NDFAIWYTPGVAEPCKEINKNPDLGYEYTNRANMVGVVTDGTRVLGLGDIGPLAGLPV
                     MEGKALLFKYLGGVDAFPICLDTKDPDEFIRTVKLLTPSFGGINLEDIENPKCFYILE
                     KLRSEMTIPVWHDDQQGTAAVTLAGLINALKLVNKKIEDVKITMIGIGAANVCIVRML
                     IKAGADPKKMIVVDSKGILNRKRDDIKSTHKEKLEFCGTTNAENRVGGMAEAMKGQDV
                     VIALSKSGPDTIQKEWISDMADNAIVFACANPIPEIWPWEAKEAGARIVATGRSDFPN
                     QVNNSVGFPAIFRGTLDVMARTITDEMCIAAAIELAKCAEDKGLREDYLLPTMDEWEV
                     FPREAVAVAKKAMEQGVARLKFSEQELFKTAELKIRRARDQVAILMEKGIIEPYKE"
     gene            16087..16926
                     /locus_tag="PK125_04440"
     CDS             16087..16926
                     /locus_tag="PK125_04440"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF004885.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HPB37390.1"
                     /translation="MKEVHVDEIVSVIEKLFIDANYNLSQNVLDTIKKSIDKEESSVG
                     KEVLRELIKNANLARDEHIPICQDTGLAVTFMEVGQDVHIIGGSLNDAINEGVRRGYA
                     KGYLRKSACHPFTRKNTGDNTPAIIHVKIVPGDKIKITVLPKGGGSENYGSVRMLVPS
                     EGKEGVKALVLEMVTKGGPNPCPPIIVGVGIGGNFETSALLSKEALMEPLGKRSDDPE
                     IAALEEELLWAINKTGIGPQGYGGIVTALDVHVKMMPCHIASLPVAVNIQCHAHRIKE
                     AII"
     gene            17077..17628
                     /locus_tag="PK125_04445"
     CDS             17077..17628
                     /locus_tag="PK125_04445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016293277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S-containing hydro-lyase"
                     /protein_id="HPB37391.1"
                     /translation="MEIKRIKTPLEPYVIEQLKAGEKVFLSGYIYTSRDAAHKRFVDT
                     LNEGGRLPFDIQNQVIYYCGPSPAPPGRVIGACGPTTSSRMDAYAPRLVSLGLKGMIG
                     KGKRSQAVKDALKQYKAVYFGATGGAGALLSKSVISSEVVAYEELGPEAVVKLGVVDM
                     PLFVINDVYGNDLYESGAERYKR"
     gene            17693..18784
                     /locus_tag="PK125_04450"
     CDS             17693..18784
                     /locus_tag="PK125_04450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018287644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-3-phosphate synthase"
                     /protein_id="HPB37392.1"
                     /translation="MGEIHVAIVGVGNCASSIVQGLAYYEKHHTATIGLMHQSICGYE
                     PKDIKIVAAFDVDKRKVGRPLEEAIFTSPNCTKVFEDQIRQSGVIVRMGNPLDGISSH
                     MADYPENRTFLVSENKPDDVARILKDTGTEIVLNYLPVGSEEATRYYAECCIESGASL
                     INCIPVFIASDEKWVARFREKGIPLVGDDVKSQIGATIIHRTLAKLFHDRGVQIDRTY
                     QLNTGGNTDFLNMLNRNRLISKKISKTEAVQSQLDMPIPSENIHIGPSDYVPWQNDNK
                     ICFMRIEGRIFGDVPIDIELRLSVEDSPNSAGCMIDAIRCCKVARDRKISGVLESVSA
                     YTMKHPIHQFPDEVARQMVEEFIRGERER"
     gene            18793..19368
                     /locus_tag="PK125_04455"
     CDS             18793..19368
                     /locus_tag="PK125_04455"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013250.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HPB37393.1"
                     /translation="MISNKVGHSLDPAILKLYRFFFAGTTINPNYLTLIGAIFGYASA
                     MCIAFGYVKTGGVVLLFSGFFDLLDGAIARSTNTVSRFGGFLDSVLDRYTDLFLMFGI
                     FVFFLKQGQLYALVLTFVATIGVAIIPYARARAEAASIECRAGILERPERLVILLIGI
                     FFNLLLYAVFVLAVLSHVTVIQRIFFVKKNS"
     gene            complement(19365..20471)
                     /gene="ispG"
                     /locus_tag="PK125_04460"
                     /gene_synonym="gcpE"
     CDS             complement(19365..20471)
                     /gene="ispG"
                     /locus_tag="PK125_04460"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: protein motif:HMM:NF001540.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HPB37394.1"
                     /translation="MNRKNDKSQYNKAIRDIERKKTKKIKIGSVAVGGNAPIVVQSML
                     KTNPENFQETLTQARDLKNAGCELIRIALPHEDTCQILPFLRREIDVPIIGDIHFNHK
                     IALKAMETGIDAIRINPGTINNVRKVREIAKNAKETKTPIRIGINIGSIEKRILKTNG
                     KPDAGSMVESALYSIKLFEDEGWTQLKVSLKASDIFQTIKAYKIFASVSDYPLHIGIT
                     EAGPVFSGAIKSAVGIGILLYEGIGDTIRVSLTGNPVYEVIAAYHILRDLGLRKRGIN
                     IISCPTCGRCSTNLKEIVEEFEKDVANIDKHLNVAIMGCEVNGPGEAREADIGIAFGA
                     NKAILFSKGDIIRTGIPKEMAKDLLKEEIFNRIS"
     gene            complement(20499..20846)
                     /locus_tag="PK125_04465"
     CDS             complement(20499..20846)
                     /locus_tag="PK125_04465"
                     /inference="COORDINATES: protein motif:HMM:NF014448.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein MoaE"
                     /protein_id="HPB37395.1"
                     /translation="MIQKWIEEIKSKSPKDQLGMILVHNGIVRATSKDGKPVTGMVLS
                     FDEKLLNTLVARYKKNDGIVDIKIWVNQGKLSIGDDIMVVLVAGRYRTDVLPAFQELI
                     REIKNNVVNEKET"
     assembly_gap    20970..21069
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(21715..23169)
                     /locus_tag="PK125_04470"
     CDS             complement(21715..23169)
                     /locus_tag="PK125_04470"
                     /inference="COORDINATES: protein motif:HMM:NF012686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB37396.1"
                     /translation="MENYIPMAITILVIYLLMNVGVGLYGMRKIKSAESYYTSGHAMG
                     PWAVALAFGATCYSATLIIGFGGKAYDVGASLMWVAASNLLFMTYLVWALLGKRVRKL
                     QEKVGAFTVTEMLVKRYRTEALRPVLAIVVAFSMIAYTVAVLTGTARLFEVLFHIPFF
                     WALIIVIFVIGFYVTTGGIVSTVYNDFLQGIVMFIGMVLLFLFVYSRVGFTSGYELLG
                     KTFGETFVSMPGNITWWSLISICLVTSLIPWGMPQIVHYMFIARDAKAVNRAIPIVTI
                     WTFVIVYAVYYVGPMARVILGAGIHTDKVIPTLIATIMPPLAIGIILSSVAAASMSTM
                     AGTSLQASFALSRDLYQKTISRGTSDEKILFISKIITICVIVFSALLAYKQLANIAET
                     FMIGAAASCASFIPILLCGLYWKRGTGKGAIASSILGFVTVVIWSLLFGSDGQGAGGI
                     HAIIPGQIVSWLTFPIASLLTKPLDPEFIRKIMD"
     gene            complement(23292..>23712)
                     /locus_tag="PK125_04475"
     CDS             complement(23292..>23712)
                     /locus_tag="PK125_04475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB37397.1"
                     /translation="VHRKPSKAVVVMPGKGTSGSAYFPEVLFFLGVFQKTGFFYVRKG
                     RRKKDNCFGLIKTLTRKVPKYDNMAKATHKRLCNFSGITFSIHPGCFKLNRRLVMLGL
                     SWGVFFAYIIGLATVVVSIIHPFIASSESWDIKKIED"
BASE COUNT         6496 a         5064 c         5492 g         6560 t
ORIGIN      
        1 tatcagtaat catctttttc aaaattaccc aacaaaaggc ggacagacgg tgttgtccgt
       61 tttagtataa agagcgcaga cttaggaaga ttcaaacatt ttagataaaa agtagaatct
      121 gatggaaaaa tggctcagta tgccaatact tatgaatacg ccatcaatca ataaaaaatg
      181 gagaatcgga tatggctgaa caagaggaaa aacaagattg tgccgggggt aagaatctcc
      241 acatttatcc tgcctggcta tggttgttgt gaactgacaa gcatcaatat tggatgattt
      301 acatggtttt tttaacttct tacaccttaa ttgctacccc ttttatggca taatcctgcc
      361 tggttcagtt acttattctt ttcaagatgt tgtctcttga tttctctttt caaaactttt
      421 cctgccgtac tttttggaaa ttctgtagtg aaaatatact ctttcggtac cttgaagggg
      481 gcaaggcggg ttttgaggaa ggccttcagc ccatcagcgg ttatcgagac atctactttc
      541 gggattatgt acgctacaac acgttccccc caaaccttat caggtgcacc tatcactgca
      601 cattctgcta ccccttcgtt tagataaatt gcctcctcca cttcccgagg ataaacattt
      661 tctccacctg taatgataag atctttgagc cggtccacga tagtcaaata gccatcctca
      721 tcgagaacgc ctatatcgcc agaacggagc catcctttct cccaaaaagc ttcttcagtg
      781 gcctccgggt tattgaggta acccttcatc atatttgggc ccctcatgca gacttcccct
      841 tctttccctg tttcaagaat gttccctgca gcatccctta tctgaacctc gcaaccggga
      901 acaacagtcc ctacagaacc aggaacatga cggtacatgt gattgaaagt gactgccgat
      961 gcagtctcgg tcataccata tccctcatgc atgttaagac cggtccgctc tttccattgc
     1021 ctcataattt caactgcaat agcagatcct gaacagaaac aataacggat attgcgcatg
     1081 atatgttcga gatgatcgag cgtgacaaaa cgggtataaa cagttggaac tgcgaagaac
     1141 ttagtaacct gaccggcctc gatgaccgaa agaactgttt ccatgtcgaa accaggcaga
     1201 atttcaagac aggcgccact cattatcgta gatgtcatga tatgaacgac ggcgaagaca
     1261 tgatgaagtg ggagtgcgca gagggcccga tcaccctggg ttgttcgctc acagaacgcg
     1321 acattgtgag cgggatgatt aatgttctca tgggtgagca taacaccttt cggcatgccg
     1381 gtggttccgc ctgtatagag aattacagca gtatctgtcc tatccctttc taaagaccgg
     1441 aacgatgata gaccagagct gatgacatga tcaaaagtaa tatcaccatc cgggcagatc
     1501 actttttcta tcccctgatt tccgcgaagt ccgtctatat ctttgagccg ttcactgttc
     1561 gtaaagagga atcttggctt tgaatgctca aggagcatcc tgagttcgtc tgacgagagg
     1621 agactggcga ggcttatcac aactcctccg gctttaagta tggcaaaata cgatatgggc
     1681 cattcctcgg aattcgatgc acagatggcg acatgttcgc ctggtcttag tcccatcttt
     1741 aagagcccag tggcgagtct gttcgttcta tcgttaaact cagcgtatgt catttccttt
     1801 tctcctgtga caagggctgg gctttttggg aaatagagtg tcgatgtctc aagattggct
     1861 gctacattca tcggtatctc ctcatatgat ttcttttgga tgtgctatcc actcagaaaa
     1921 gcgtaccttt acatggctcc ttatgtcaag ataggattac gaatatattt ttctatggtc
     1981 ttgtaagcag tagatgacga tgtgcctatc tgattatgac ttgaaatggg gggtgatttt
     2041 gcgatcccgt atattatgat agtaatgagg gatagcctta aatacaaata tgatggctgc
     2101 cagcattatt aatagggtgc tgtatcgata tatttctgcg agagacacct tatcaagaag
     2161 aaagccaatg ataagggaga tagctccaat acccaaatca taggagaaaa ggtaggtgga
     2221 atttgcagcc cccctctcgc tggatttgac taaattattt atagcagcct ggcctgtggg
     2281 cataagaatg ccgaaaccaa aaccattaat tacccctgca accaagaact gtgttgagtt
     2341 gccggcatat ccaagccaaa gaactccagt tgatgccatg attaggccgg ccactatcag
     2401 atgcaaggcg tggccagtgt caaataatct acctgcaaac aaacgtgata gaaaaacagc
     2461 tactgacaaa acaacgaaga aggtggccac attcggaaaa tttttttgcg aggcgtaaat
     2521 tgatacaaaa atgattattg ccccatatac agccataata aagaacatac acagtgaaat
     2581 gggaagagcc atcttgctga aaagatccgg gagggagaat ttttttctgg ctggggactt
     2641 ataagggtat ctcgtacaga atgcaccgag gaccgaaaga aaagaaataa cggtaattgc
     2701 aagaaacaaa gtgttggggc cctggttttt tatcaactcc aggccgaaca tgggaccgat
     2761 cgtcatacca acaggaaagc ttaaagcgaa aaccccaatg ccctgaccta ttcgagaagg
     2821 cggtacaatg tctgcaacaa ttgtagcact tgcagaactt gtaattccga agaatgtgcc
     2881 atggataaaa cgaatcgtga acatacctgc tacggtactg atcagaggat aaactccgta
     2941 cagtgcggtt atcagtgaaa gagaaagaac aaatacgcct ggcctgtgat aattgtccat
     3001 tatataacct gcaacagggc gtataaggat tactgagatg gaaaaggctg ctataaccag
     3061 accaacactg ctgtgactca tttttagatg ttctaaaaga taaatgggca gtgtaggcat
     3121 cagggcgtag aatgccagcg aattaaacag acttgaagcg ataatggcta tgaattcctt
     3181 tgaccagagc tctctttgca tttacaatct gaagtttagt atctaaactt gccggggtaa
     3241 gacgttttgg gttttctacc atgctacagg ccttgtgtca atcgctttcg gttgttgttc
     3301 ccggtggtat ggaatggtat actgatagga aagcttaaaa atatacaaga cagatatgga
     3361 gacatcccat gaatgaccaa atagagaata ttgccgagtt gattgttaat gcgaagagaa
     3421 tcgtcgtatt tgttggtgca ggattgagca ctgaatcagg catccctgat tttagaagtc
     3481 cggggggtgt ctgggataag tacgaccctg aggatttcta ttttcagaat tttgtctcca
     3541 gtgaggcatc ccgtgaaaaa tactggcaga tggcaactga gatgtatgat tccatgaaag
     3601 atgctatgcc caatcaggca catcttgcag tggcagagct ggaaaaactc ggcaaattgg
     3661 actgcctgat aacgcaaaac atagatggtc tccacttcaa ggccggaaac acacctgata
     3721 aggttttgga actccatggt actgccatgt atgtcacgtg cctgaattgc aataagcggt
     3781 atgatcgtga agaggttcaa ctccggatag caaaaggtga aaaagcccct cgatgcgatg
     3841 aatgcactgg acccctgaag ccggccacta tttcctttgg ccagtctatg cctgagcagg
     3901 agacacggga agcgtatgag cgctcaggca cgtgcgatct tttcatcgtc atagggtcat
     3961 ctctcgtggt acagccggca gcttacatgc ctgtggttgc ccacaaaagc ggtgcaaagt
     4021 tagttattat caaccgcgat gagacatcat gcgatgatat tgcagatatc gtcgttaagg
     4081 cacaggcagg cccaaccatg gcatcagtgc tggcaagggt ccgtgaagca ttgtcaaaaa
     4141 aacaatgaat agaacaaatt tttagaggtt atgaatccct tgcctggaat gtatcttgct
     4201 gatgatcctc atctttaatc ttcgtggcca ataaacaggg cttcgtagcg tgtaccatgc
     4261 attaacagcg ctggcaagtt gttcgctctt taacccctcc ttgccataga tctcaatagt
     4321 taatgcatta atgatggttt cgatctccat tttcaattta ggaaattgac cgcagagctt
     4381 aatgccaaat tcgaaagggg tatcagtcat tagatgcggc agtccgctgc gcctacccca
     4441 ttctgtaagc actgaaaaaa gttcgcttgc ccttttgtac ccttgagcaa cgccaagcac
     4501 tgctttccat aaagaatgta aaaattgcca cagtcgcttg taccatggca gaaaatgatt
     4561 tttattcctg gcagtttctg aattgattgc agtcctggag aaaagccatc tcatgatcag
     4621 gtatattaga agggctatag taaaggcgaa gaacataatg gctattatct caattccctt
     4681 tcgaagaatt acttccagaa gctccatcca ccagctgtct gcgaacaatg attcagatcc
     4741 aaaatctttt ccagggggtg atcctgacga aggatccgat ctgacctgcc cacccatgaa
     4801 cataaagcgt actacactgc tcacaaccgg gagaatccat aaagccaggc ccttaagaac
     4861 acgttgccca gtatcggcca cctgggtcag gacaggtaaa agaagaaata cagaggttga
     4921 gacgatgaga ataactacag aaacagtgct cataagaatt cctgatcccc ctcggccagg
     4981 caggaatcgc cgtactcctc catgtccaac ccttgttatc cctatggcta tgatgctgag
     5041 gagcaaaaat ggatagatca atgctgaaga tagagaatca tccacattta taccgccctt
     5101 cacacgcagt gtgagctttg cgaggagcag aataaagaac gctgcaagcc caatgtcaaa
     5161 gcgtgcacat attaccgagt aggtctttct tctttttgcg aatgcagttc cagccaacca
     5221 ggtgagacca acacagatta ggataaaaat atgatgaacc cagtccatgg gctcccgagc
     5281 aacagtaaaa aaaaggctga tccagtgggt attaaaaatc gggtaagagg gataaaagat
     5341 cccatgaagg acatatgcaa ctgccaggga acatccaagg atatgtgtta ttatcagcgt
     5401 tacaactctc catccatgtc ctgtggacac gttggtaaga actattgcaa aagaaaatgc
     5461 tgctgctgtt tcaagaaggg agatggtatt tcccagtacc gcagtcatgg tgaaggacac
     5521 ccaagaataa atccaacaaa tttccatggc tcctgttatg agagacaaaa gagcactgtt
     5581 tttcaccttc atatgccctg aacctcttcg acacctataa gcccgttgat aagaggggcg
     5641 tgtatttctg gagcaactgg attgtttttc ctgagtaatg aatccatttc ccaaggtacc
     5701 attatcaccg gtattttcct ttgtttcaga tattcaacaa ttatctttgt actatcatca
     5761 ttatttaatg aaaggcaaag acatgtagtg ccccagggaa ccgcgccagt ggtttccaag
     5821 atatctataa aaacgttgtt ttgtactggt tgcagtcgtg cgagtgcttc caaaatcctg
     5881 gccaattgtt gggaacctct tgaaactgca acagacgatg aagatccttt cacctcgcca
     5941 tttgttagga ggcccatagc acaaccccgc cgatcaaatt gcagagcgag agatgcaatt
     6001 atttcaaggc cctgttcaaa ggattctttg ttctctgctt ttgtaaaccc ctcaatattg
     6061 ataagaaaaa gaatcttttc ctgcactgtt gagtcgaata ccttctcctg taaacgttga
     6121 tgcctcgcac tagccttcca gtgtatgtat cgtgcaggtc ttccataatg gtaatcggag
     6181 gtgccaagga tgtagaccgg atcatcaact gggctttcac ccccaggggc accgaagaat
     6241 tctctttttg ggagactaaa taatggaagg ggcacaattt ttggataaac gacaatctcg
     6301 gctgtctccc tgtctgttga ttcgaactgg aaaaagccaa gtaggtcgcc tgatagcact
     6361 tgcaaaggtc ctacttcata aacgcccctt ttaagagtag cagtctgcca ctcaaatagg
     6421 gcttgctgat accataaaag agtctgttga ccatagaagg ttccagtttc agtaacagcg
     6481 tttgtggatg catgaacagg catttcaatg tccagccaca caggcaaggg tttgtggttc
     6541 tcgacagtca gggtgaaagt aatattttct tctggaaaga tccttgtcct gtcggaaata
     6601 aggcggcata cgatcttttt tcccgccatt tttgcccata ccctgaggat gatagcaatg
     6661 ccaaaaacga ggatacacag gattgaaaga tcatgctggc ggttaaggag gcctataaaa
     6721 aggaaaatgc ccaccgctac catcatgatc gaagttataa agagactcgg tacccgccgg
     6781 gctaaagtag tgttcataag taatgattat ttttctgaga ctggcactga aattttgttt
     6841 aagatctgat tcacaatatc ttcctgcgat gtattcctga gccgctcctc ctctctgagg
     6901 atcattctat gagaaagtac aggcacagca agtgatttta tatcatcagg gagcacatat
     6961 tctcgacctc tcaaagcggc aagggcctgt ccggatcgca tcagccccag agaacctctc
     7021 gggctggaac cgagtctcag caaagggttt atacgggttg ctccaacaat tgaagttatg
     7081 tattccttga ctgctccgga cacgtgtatc tttcttctgg ccttctgcaa gcgggagata
     7141 tccgcagggg ttgccactgc tgaaagagtt tgaagaggat cttcctcctg aaaacgttga
     7201 agaatagcaa tctcctcgtc ctgatccgga taaccgagcc gtattctcag gagaaaacga
     7261 tcgagttgcg cttcaggcag tgggaaagtt ccctcaagtt cgatgggatt ttgtgttgcc
     7321 atgacgaaga aagggtgggg cagagaatga gtttttccgt ccactgtaac ctggcgttct
     7381 tccatacttt caagaaggct ggactgtgtc cggggtatgg ttctattaat ctcatctgcg
     7441 agaagtacat ttgtcatgac cggtccctgt tgaaacacaa attggcctga acgttggtca
     7501 aacacattga agccggttat atcggcaggc aacaggtctg gggtgaactg aactcttttg
     7561 aaagatgcgc ctatgctctt tgccaatgac ttggcgatca gtgtcttgcc gagaccgggg
     7621 atgtcttcaa tcagaatatg gccttctgcg ataaggccta ccagaagaag ttctatggcc
     7681 tgttccttgc ccactatgac actggacata ttccctgtaa tctgtttaca taaatcgaaa
     7741 gtcatatctt ctccttattg attcccaccc ttgtaatccc ctgatcctga ttttgctgca
     7801 gatttcacat ctcgagggat tgaaatatgg accgtatctc gcagggtact cccctgaaat
     7861 cgagctgttc gataatctca ttcatggtga aaccccttgt cttgcgaaag gatgccttgc
     7921 cctgtgcgca aagcttgcct gtctcatctt cgatcattgc ggaaagataa tacatattgt
     7981 gagttgatga tacaaattgc cccgaggcgc gatatgtctt tccgcacatg atgggccttt
     8041 taaattctgc ttccattttc catgttgcgc aaatcttttt tgtcttcatg aacagtgccc
     8101 accacattac ttcatcaagt attccggtca cgataccacc atgcaacatc ccatggtaac
     8161 cctgaaatct ttcctgggcc gtgaattcgc atgaggctgt gctatccata tatttcagct
     8221 cgaggccgag accgcctgtg gcgggtccgc aaaagaaaca ttttttatat ccaggtaaaa
     8281 gtcccttcat ggattgatct tccctccctg ctttcctctc cgtattgtaa catttttaat
     8341 aaaaaggtgt agagaaaagt catggcttct ggcctaactg tattcgactt gaaaatgctc
     8401 tttacagggt tgtttatttc ttggcggagc cgcttttgag ggctttggca tatgcaaaga
     8461 tggcttttac atattcatcg cagtgattat atgcataaac agccttccgg ttcttatcag
     8521 ggttgttctt ttcccatccg tgagataaaa gatagtttgc aatgctcgca cacgcatcgg
     8581 gaacatcgaa gagatcaata attccgtcat tgttctcatc aacagcgtat ttccggtaag
     8641 atgagggaat aaattgtgcc attccgaatg cccctgccca tgaaccttta atggaaagtg
     8701 gattcttgtt aagcattttt gctatccaga aaagttcgat aagctcattt tcagcccatg
     8761 aggagcgtct attttctact aaggcgaggg taagaaggct attgaaaatg ggatattttc
     8821 caacgtatct gttgaagttt gtttcgagcc tgagaatggc aataataatc tctttgtcta
     8881 caccataatt tgcttcaacc cgcaccagca cttctttgtt ttcgttaagg aatttttgcc
     8941 ctcgttccag tgattttttt gttagaaggc cgaatttctt gctgaaataa ggcaggcctt
     9001 taccactccg ttcaaggatt tgaggataaa tcatcacacg ctcatcagaa aatatctgcc
     9061 gtatctcttc ctcgctaaat cccgttacta atattttagc ggtaagggtg tctatccgat
     9121 ctttgtactg agggtcaatt tccccagtaa caggggatga gaacatgaaa agaaggccta
     9181 tgacggttat tataaagcag gtgtttattg ttgtgatttt tttatctccc aatggatgag
     9241 catttttgta ttccacagat caattatcat tatgataccg aggttggaga agaaatcaag
     9301 cgttttgttt ctaaccctga tagaaactac ttttgtatat attttctctg tttttatggt
     9361 acaattcctt cgtttggaat actcagagaa aaggatttat ctgccatatg aaaaaaagcc
     9421 tcgatgaaaa agaaccggac aagatagtac ggccagcaat cccagattcg gttcaggatc
     9481 tgattgagtt tgtttcttca atagtttttg agacaggtat ggaagacgat aaggttaaaa
     9541 cgattggcct tgccattgag gaggtagtcc aaaatattat tgaatttgca tgccctgagg
     9601 gcaaagagga gataactatt tcctgtgagc ttcttgacag tggtgcaaca cttattcata
     9661 tatgggacac gggcaaacct tttaacatgc ttcttgccgg aacatttccc gaggttgagc
     9721 tggtttcggc acatgagaaa ataccatcaa caaagatgtt gaaaaaagca gtgaagaaca
     9781 tagagtacat tcgtggaaag gacaaaaaca tactaattct gaccatcccc ccggttaggg
     9841 gtcgcaactg attacaacga atccaaggcc tggatgttcg aaggtaaaag gagtcaacgt
     9901 cagggaaagg gtcgagggaa gggttctggt accccaccct gacccttttg aattaattga
     9961 agtattttat tcttttattc ctgccagacg cctagcagct ttctttttgc tctccacatc
    10021 agtttcacgg aaaatagtta cacgatgagc ctccggtgag ccaccaccat ggatatcaga
    10081 gattgtatct gcgctttcga gggcgagctt ttcagctaat cgatacattt tgaccctgtt
    10141 ttccacaggg acggtgtcaa cggctttcag gtattttttc agaaggtcgc ctatgtcagg
    10201 attctcaaaa tctctatccg atggaaggtc agccacaaaa ccaccgcatg catcgatcat
    10261 caatcgggta gcctcagcca tttctttgcc ctcatgtatt ttggaagcat tggcgagaac
    10321 agtatcgatg aagtatgccc ctgaaggctg ttgtttgcct tcataggaag aggcaagaca
    10381 gcaaccatat aaggtttcag ctctgtggat catgtcgatg actttctgtt tcaaatgacc
    10441 tgcttttgag gtcccattgt agtccattaa agtaagggct gcacccacca tacagtcaat
    10501 cttgcccgat ttgcacccac catggctctg gcggtgaaaa gaggaaaatt ttataaccat
    10561 ttcagatgca aactctgtct caccacacat gaaaacgcgt tcccagggaa caaatacatt
    10621 atcaaaaatc agggtagggc agtacttgga gtagtaggca ttaccgatgt cacagccatc
    10681 cagttcccgc atatccagac tggatctgcc gactacatgg attaatccct ttgtgtctga
    10741 aggaacggca aaggaaacgg aatagtcttt gtcgtttttt cccatggcac gggttgggat
    10801 cacgatgatt tcatgagatg aaagggaacc cgtctggtgg gccttggcac cccggacaac
    10861 aataccgtcg tcacgttttt ctacaacacg gacaaacatg tctttgtcca cttgttcgtg
    10921 tggagccagt gaccgatcgc ctttcacgtc tgtgactccg gcattcgcag ttaagtcatt
    10981 cttctgcatg tgcttcaaaa actccgtgaa attcttgttg tagttagtgc catatttttt
    11041 atcgatatca taggttacga ttgcaagagt tgagagacaa tcaagtcctg tgcatctctg
    11101 atgacaagtt ccgacgtagt tagcaacttt gcggttcact tttacccttg ctactagatc
    11161 ttctatgctg gcggggggca atgtgaaccg actaaccggt tcattaatga gagggcttat
    11221 tgtgatcata aggtccttgt tctccggcat attagcaagc tcgtaggtgg cgccagtagc
    11281 ctctataccc gcacgaaggc gtgggttatc cacaatgctg gtgagtttct taccaaacat
    11341 gtaagctgtg ggcttgagtg ccccaatcga tgcaatgtat tcttcttttg ttttaatacc
    11401 catactacat tccctcctcg ttttaccatt atctggtctt aggtttatat ttcagaatgg
    11461 agacctttcc tgctcagcca gtatcttttt aacgctcgat aggacctgta tcttcacatt
    11521 agtgagatga ttcttgagga cattctgtgc cctttcaaga tttcgtaagg caactgcatc
    11581 atatatttct tgatgctcct gatctgtagc tgtaagagat gacatgggca gataattacc
    11641 accgtattta aggaataaca tgtcgaagac attctgcaga atccgtatct gtgtctcttt
    11701 ttcagagaga gacgcaaggg tcatgtgaaa ttccttattt ttaaaaagcc tttcctttaa
    11761 atagaactcc cgttctgctg aaaagtgtgc ttccaacgct gtcttcaggt ttttcagccc
    11821 atccctgttg atatgctgaa tggtggcagg tatcagggat ggttcaatga gttcacgaag
    11881 ctcatagagc tcttcaatct ctttaaggct aaagggcgcc atagaatatc cccgttttgg
    11941 ctcatgaagc acgaaaccct gcaattcgag ccattttaat gcctgtatga taggcgttgg
    12001 gctcaatttc aacttctcag cgaggtcccg gtaggctatc ttttgtccgg gagctagttc
    12061 cttggtgtac agcatgcgtc gaataccttg atatgctatt tggctgctgt ctgcctgtgg
    12121 ttttgttgtt tttttggttt tgggtttcat aataggtcat tgtatatcaa ttaaattaaa
    12181 tttgcaatta aattttataa tggcaaagtt ttgtattatc tattccggca acaacatttc
    12241 tttgcgttga tctttgcaac ccttcctaat agaaaatacc tgattttatt gcctcatggc
    12301 aatttagctg agggtatctg gcattatctc ttgttcgggc atgctgtact caccaaaaaa
    12361 cccttcaacc cgagcgattt tgtttatttt gattcattat tgaattggat tttcaattta
    12421 ttttcattga catccagtga tcgtctgttt atacttggcc atggcacggt tttaacaaac
    12481 ctgtgtgcca gttaactcca tttcaaggga tagccatgag acttcatgag tacgaaacat
    12541 tagacatttt tgagcagaac cgaatccctg ttccccggag gggtattgcc ggaacaacgc
    12601 acgaagcgct ccagatagct ggggagattg gatttcctgt tatgctgaag gcccaggtgc
    12661 ttgcgggagg ccgtggtctt gccggtggag tcagggtagc gttgacacag gatgagctgg
    12721 aagatgtggc tgaggaaatg cttagctccg acataaaggg gttgcctgta cgtaaggtac
    12781 ttgtatgtga aaaagcagcg attgagaaag aattgtatct gggtattacg gttgacggtt
    12841 attcgggaag gcctgttatt gtagtcagta ctgaaggggg catgcttatt gaagaaacag
    12901 cgaggaattc tcctgagaag gttgcgtcaa ttcatataga tccctccttc ggctattacc
    12961 cgtatttggc ccggtccctc ctgaggagat tgggtctcaa agaacagttg atcgcttcct
    13021 ggagcgatat tatcggccag ctttataata tcgccaggcg ctatgaggca ctgattgctg
    13081 aaatcaatcc acttgtggtt cgccccaatg gtggtctcct tgccgttgat gcagtgctgg
    13141 aagttgatga ctcagcattg tcaaggatac gttttcctct tccagaccgg atagacagga
    13201 tagagaatcc tcttgaacgc cgtggccggg aaataggtgt tacttatgtt gatcttgatg
    13261 gagatatagg gcttatttca tccggtgctg gtcttggcat ggcctccatg gatataatag
    13321 gggagaagat gaagccagca aacttccttg aaacaggggg aggtataacg gctgacctgc
    13381 tttacaagtg tatggaactt atcatgatga agcctgatct cagagctata ttcattaatg
    13441 tgtacggtgg aataaacccg atccatgaag gtgccaaggg tgtggtacgt tatattcacg
    13501 aacgcaacgt aaggattcct gttgttgcga aagcccttgg gaaccgtcaa gaggagacgt
    13561 gggagatttt tcgttctgcc ggagtccacg tggtgacaga aacggcaacc gaaaaagcaa
    13621 tcgataagct atatgaactt gttgggcgag gttaagcgat gagtatattg attgacgatt
    13681 ctacgcgggt tattgtgcag ggtatcaccg gccgcattgg aagcgtccag actcactgga
    13741 tgcttgaata tggaacgagg gtagtaggag gcgttacgcc cggaaagggc ggaacaaccg
    13801 tggaaggggt accagttttt gatagcgttc ttgaagctgt gaaggagacg ggagcaaatg
    13861 catctgtgtt ttttgttcct gctgcattcg tgctcgatgc ttttttagaa acaatcgatg
    13921 cagggataaa gctgatcgtt gttgtccctg agcacattcc ggtgagagac gttatgaaga
    13981 tgcgcagcta tgctgtggaa aaaggtgtat tcgccctcgg cccgacaacg ccaggaattc
    14041 tatctcctgg aaaaggaaaa atgggtatta tgccagcatc actctttgcg ccgggtcgtg
    14101 taggaatcat ttccaggagt ggaactcttt cctatgaatt tgcaggtatc ctctcagaag
    14161 taaacgtcgg ccagagcaca gttgtgggaa tgggtgcaga tccggttgtc ttgaggaatc
    14221 tcgctgatat ccttgaatta tttgaacgag atactgatac tgatgctgtg attattgttg
    14281 gggaagtggg aggagagcag gaagaaaagg gtgccggatt tatatcgagg cggatgacaa
    14341 agccagttgc cgcctacatt gcaggccgtc agtctcccca gggtaaaaga atgggacatg
    14401 caggcgcaat tgtgagaggg gcagcaggga cggttgaggg caagcagaaa gagttgaggg
    14461 cggcaggggt ggaaatcctc gagagtccaa ttcatgtggc tgattgggca aggaagaaca
    14521 atctgagata atcatatatc ggtgagccgt cagctatcaa ttaaatgtca ataaattata
    14581 gaatcttgta tggcggtgat tcatcgccgc attggcagta ttttcatcac ctttgttcag
    14641 ttttctcttt gaattttttc taaatagcta tacaatctta taccaaactt aaacataaaa
    14701 gctgtttctc taataagtca aatgaagggg ggaatcatgg aaaaaaataa ggtaacaaag
    14761 gaagagcttc ttgagaaggc taagaaacct gcccaggatg cactcaagat gcatccatat
    14821 tataagggca agattgaggt agttcccaaa tgtgtaatta gggatatcaa cgattttgcc
    14881 atctggtata ctcccggcgt tgcagaacca tgcaaagaga tcaacaagaa ccctgatttg
    14941 ggctatgaat acacaaacag agcgaacatg gttggagttg ttactgacgg tacaagggtt
    15001 ctcgggcttg gtgatatagg gccccttgcc ggccttcctg ttatggaagg aaaagcactg
    15061 ctctttaaat atcttggcgg tgtggatgca ttccccatct gtctcgatac aaaggatcct
    15121 gacgagttta tacgaacagt gaaactcttg accccttctt ttggagggat taaccttgaa
    15181 gatatcgaga atccaaagtg tttttatata ctggaaaaat tgagatcaga aatgacaatc
    15241 ccggtttggc atgatgatca gcagggaaca gcggctgtta cactggccgg tctcatcaac
    15301 gcactgaagc tggtgaacaa aaaaattgag gatgtgaaga tcactatgat aggcattggt
    15361 gctgcaaatg tctgcattgt ccggatgctt atcaaggcgg gtgctgatcc aaaaaaaatg
    15421 atcgtagtgg acagcaaggg aatattgaac aggaaaaggg atgatattaa gtcaacacac
    15481 aaggagaagc ttgagttctg cggtacaacg aatgctgaaa acagggtggg cgggatggct
    15541 gaggccatga aaggacagga tgtggttatt gccctctcca aatctggtcc tgatactatt
    15601 caaaaggagt ggatttctga catggccgat aatgcaatcg tttttgcctg tgccaatcct
    15661 ataccggaga tttggccctg ggaggctaag gaggcaggtg cacgcatagt tgccacagga
    15721 agaagtgatt tcccgaacca ggtgaacaat tcggtcggtt ttcctgcaat cttccggggt
    15781 acactggatg taatggcaag aaccattact gacgagatgt gcatagcagc ggcaattgaa
    15841 ttggcaaaat gtgctgaaga caagggactt cgtgaggatt atcttcttcc tacaatggat
    15901 gaatgggagg tatttccaag ggaggcggtt gcagttgcaa aaaaggcaat ggagcaggga
    15961 gtagcacggc ttaaattctc agaacaggaa cttttcaaaa cagctgaatt gaagatcagg
    16021 cgagccaggg atcaggttgc catactgatg gagaaaggga taatagaacc atacaaggag
    16081 tgatgtatga aagaggtgca tgttgacgaa atagtatcgg tcatagagaa actgtttatt
    16141 gatgcaaact acaatctttc tcaaaatgtc ctggatacca tcaagaaatc gatcgacaaa
    16201 gaagagtctt ctgtaggtaa agaggtttta agggagctca taaagaatgc aaatttggca
    16261 agggatgaac atattccaat ctgccaggac acaggccttg ctgtgacatt catggaggta
    16321 ggccaggatg ttcatatcat tgggggatca ctcaatgatg caatcaatga gggggtgcgg
    16381 agaggctatg ccaaaggcta cctgaggaag tcagcatgtc atccttttac acggaagaac
    16441 accggtgaca atacccccgc catcatacac gtgaagattg tacctgggga taaaataaag
    16501 attaccgtgc ttcctaaagg tggtggaagt gaaaattatg gatctgtaag gatgcttgtt
    16561 ccctcggagg gaaaggaagg ggtgaaggca cttgtcctcg aaatggtaac gaagggaggt
    16621 cccaacccgt gtccgccgat tattgttggt gttgggatag gcggtaattt cgagacatca
    16681 gctcttcttt caaaagaggc tctgatggag cccctgggaa agcggagcga tgatcctgaa
    16741 atagctgctc tggaagaaga actcttgtgg gcaatcaata agactggcat tggtcctcaa
    16801 ggttatggtg gtatagttac agctctcgat gtccatgtta aaatgatgcc atgccatatt
    16861 gcctcattgc ccgtggctgt taatatccag tgtcatgcac accggataaa agaagccatc
    16921 atctaataag ggtgtcttca aatgggcaaa ggaagaggga aaaggcacgg gaagaaaaga
    16981 tgagtctgtt acacagcaca atgattgtgt tcaccagttg ggttgaaggg tgaagtgtga
    17041 tcaccccaac ttaaatcggt tgaaggagag tagcccatgg aaattaaaag aataaaaacg
    17101 ccactggaac cttatgttat cgaacaattg aaagcagggg aaaaggtatt tttgagcggt
    17161 tatatctata cctcaaggga tgcggctcat aaaagattcg tcgacacact taatgaaggt
    17221 ggaaggctac cctttgatat ccaaaaccag gttatctatt attgcgggcc atctccagcg
    17281 ccacccggca gggtcattgg tgcatgcggg ccaacgacaa gctccaggat ggatgcatat
    17341 gcgcccagac ttgtttccct tggcctcaaa gggatgatag gaaaagggaa acgatctcaa
    17401 gcggtcaaag atgcgcttaa gcaatacaaa gcagtatatt ttggagcaac aggcggggca
    17461 ggggctctgc tttcaaaaag tgttatatct tcagaagtgg tagcttacga agagcttggc
    17521 ccggaagccg ttgtaaagct gggtgttgtt gatatgcccc tctttgtgat taatgatgtt
    17581 tatggcaacg acttatacga gtcaggcgct gagcgttata aaagataggc atgtgtcgcg
    17641 tatcctttga ctttttaaaa caaatagttt agataagaat tcacggggaa atatgggaga
    17701 aattcatgtt gctatagttg gcgtaggaaa ctgtgcaagt tcaattgttc agggattggc
    17761 ctattatgaa aaacaccata cagcgacaat cgggctcatg caccagagta tctgcggtta
    17821 tgagccaaag gatataaaaa ttgttgcggc ctttgatgtg gataagagaa aagtaggaag
    17881 gcctttagaa gaggcaatct ttacttcccc caattgcacg aaggtttttg aggatcagat
    17941 caggcagtct ggagtgatag tccgcatggg caaccctctc gatggcatct cgtcccatat
    18001 ggcggattat cccgaaaata gaacatttct tgtttctgaa aacaagcccg atgacgttgc
    18061 aaggatatta aaggatacag gaaccgagat agtgctcaac tatctccctg ttggttctga
    18121 ggaggcaacg aggtattatg ccgaatgctg cattgaaagc ggagcaagcc ttatcaattg
    18181 cattccagtg ttcattgcat cggacgaaaa atgggttgcc agattcaggg aaaaaggcat
    18241 tcctttggtt ggtgatgatg taaaatcaca gatcggggca accatcattc acagaaccct
    18301 cgcaaagctc tttcatgata gaggcgtcca gattgaccga acctaccagt tgaacaccgg
    18361 cggtaataca gattttctga atatgttgaa caggaatcgc ctcatttcaa aaaaaatctc
    18421 caagacagag gctgttcaat cgcagcttga tatgcccatt ccatctgaga acatccacat
    18481 cggtccttct gactacgttc cttggcagaa cgataataag atttgcttta tgaggattga
    18541 aggtcgaatt tttggcgatg ttcccattga tatagaactg cgtttatcgg tagaggactc
    18601 tccgaatagc gcaggatgca tgatcgatgc tataaggtgc tgcaaggtgg cacgagacag
    18661 aaaaattagc ggtgtccttg aatcggtatc tgcgtacacg atgaaacatc ccattcatca
    18721 gtttcctgat gaggttgccc gccaaatggt ggaagagttt attcggggag aaagggaacg
    18781 atagggtagc cgttgataag caataaagtt ggtcattccc ttgatcctgc tattctgaaa
    18841 ctttatcgat tcttctttgc cggcacaacg ataaatccga attacctgac cctgattgga
    18901 gcaatatttg gctatgcctc agcaatgtgc attgcctttg gctatgttaa gacaggggga
    18961 gttgtccttc tcttttcagg ttttttcgat cttcttgatg gcgccatagc aagaagcaca
    19021 aacactgttt cacgtttcgg aggatttctt gattcggtcc tcgacagata cacggatctt
    19081 tttttgatgt ttggaatctt cgtgtttttt ctgaaacagg gtcaactgta tgcccttgta
    19141 ttaacctttg ttgcaactat tggggttgca attatcccct atgcccgggc acgggcagag
    19201 gcagcatcca tagaatgcag ggcaggtatt ctggagaggc cggagaggtt agtgatcctt
    19261 ctcataggta ttttttttaa tctgcttctc tatgccgttt tcgtgcttgc tgttctcagt
    19321 cacgttacgg taatacaaag aatttttttt gtgaaaaaaa acagctaact gatcctgtta
    19381 aagatctctt ccttcaagag atcttttgcc atctccttgg gaatgcctgt tcttatgata
    19441 tcaccctttg agaagagtat ggccttgtta gctccgaaag caataccgat atcagcctct
    19501 ctggcctcgc cagggccatt cacctcacag cccatgatgg ccacattgag gtgcttgtca
    19561 atgttggcta catctttctc aaattcttct acaatctctt tcaaatttgt actgcatcgt
    19621 ccgcaggttg gacacgagat gatattaatt cctctttttc ttaaaccaag gtctctcagg
    19681 atatggtagg cagcgatgac ctcataaaca ggatttcctg tcagtgaaac ccttatggtg
    19741 tcaccaatgc cttcgtaaag aagaattcct atgcccacag cagacttgat agctcccgag
    19801 aacacaggcc ctgcctcggt aatccctata tggagaggat aatcagagac gctggcaaat
    19861 attttatatg cttttattgt ctgaaaaata tctgatgctt tcaacgaaac cttcagttgt
    19921 gtccagccct catcttcaaa taattttata ctgtataaag cgctttccac cattgatcct
    19981 gcatcaggtt tgccattagt cttgaggatt cttttttcaa tggatccaat gttgatgcct
    20041 atgcgaatag gggtttttgt ctcttttgca ttcttggcga tttcccgcac cttcctgacg
    20101 ttgttaatag tcccgggatt tatccgaata gcatctatgc ctgtttccat ggccttgagt
    20161 gcaatcttat ggttgaagtg gatatctcca atgattggaa cgtcaatctc ccttcgaaga
    20221 aaaggtaata tctgacacgt gtcttcatgg ggaagggcta tccgaataag ctcacacccg
    20281 gcatttttca aatccctcgc ctgtgtcaac gtttcttgaa agttctcagg atttgttttg
    20341 agcatagact gaacaacgat aggtgcattt cctccaactg ctacgcttcc tatttttatc
    20401 ttctttgttt tcttgcgctc tatgtcccgt attgctttgt tatattgcga tttatcattt
    20461 tttcggttca ctgttcacca ttcactgatc actgtatttc atgtttcctt ttcattgacc
    20521 acattgtttt tgatctcgcg aataagttcc tggaaggcgg ggagaacgtc tgtcctgtat
    20581 ctgcctgcaa caaggacaac cataatgtca tcgccaatgg ataatttacc ctgattaacc
    20641 cagatcttta tatctactat gccatcattt tttttatacc gggcaaccaa ggtgttcaaa
    20701 agtttctcat caaaggagag caccatccca gtcaccggtt ttccatcttt tgaggttgcc
    20761 ctcacaatcc cgttatgaac caggatcatc ccaagctggt cttttgggct tttagatttt
    20821 atttcttcaa tccatttttg tatcataagt tatcccccgc gttctcattc tatacctggc
    20881 tttcatggtt gtcaatgaca gaaattgtat tcaaatttat cggccatgtt tttgttaccc
    20941 cctttgatag gaagggtaca gttgtggttn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    21001 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    21061 nnnnnnnnna tttatcggcc atgtttttgt tacccccttt gataggaagg gtacagttgt
    21121 ggttttgtta tgtttttaat accaaataaa atcattttga tctcattcat ttgcaaatac
    21181 acggtgtatg cctctcccag gggggggtaa cgtggcactt atcccaacag ccaaggcaaa
    21241 acttttgtga agccaattga gttcgatctc cagttgatct attttcccga agttccgctt
    21301 aatcgttctc catggtcctt gcctgaatgg ccactctccc ctgaaaagtt caggtgagtc
    21361 ctttatcgga agagccttcc aattccgtgt caggccagga tacgctggga atggcttgtc
    21421 agattcgcag aggtcgcccc ctcctgtatg cttcaaaggt tacctttgct ttgaatgacg
    21481 atggatgttc ggctctcatc ctttgcatca caaaaacctc aagattttag atcaggacgt
    21541 gatttaatat tatgtccaaa attgtggttt cagtgtagcc gtatgcgtct ccttgaaaaa
    21601 tgaagagtta tcatggcgaa cagtttaaga gagattcggg aacgaaaatc gacattcccc
    21661 tcttataata atatctgttt cttactggca cggtgtatag aagcccggga cctatcaatc
    21721 catgatcttc cggatgaact ccggatccag tggtttggtc agcaaactgg cgatcgggaa
    21781 ggtcagccag ctaacgattt gtcccggaat aattgcgtgg atgcctcctg ccccctgtcc
    21841 atctgagcca aaaagaagtg accatatgac aacggtgaca aatcccagaa tcgagcttgc
    21901 aattgcgccc ttaccagttc cccgcttcca atagaggccg caaagaagga tgggaataaa
    21961 ggatgcacaa cttgccgcag ctcctatcat aaaagtttct gcaatattcg cgagttgctt
    22021 atatgcaagt aatgccgaaa aaacaatgac gcatatagtg atgattttgg aaataaatag
    22081 tattttctcg tcgctggtac ccctgcttat ggttttctgg tacagatcac gtgaaagggc
    22141 gaaagatgcc tgtaaggatg taccggccat agtagacatc gatgcagctg ccactgacga
    22201 aaggattatt ccaatggcaa ggggaggcat gatggtagcg ataagggtgg gaattacttt
    22261 atcagtatga attccagcgc ccaaaatgac tcttgccatc ggtccgacat aataaactgc
    22321 gtaaacgatt acaaacgtcc agattgtgac gatgggaatt gcccggttca cagcttttgc
    22381 atctctggcg atgaacatgt aatgaactat ctgtggcata ccccatggga tgagacttgt
    22441 gacgagacag atagaaatga ggctccacca cgttatgttc cccggcatag aaacgaatgt
    22501 ttccccgaag gtcttcccta aaagctcgta tccggaagta aagccgacac gggaatagac
    22561 gaacaggaaa aggagtacca ttccaatgaa catgacgatt ccctggagaa aatcgttgta
    22621 taccgttgaa acgattccac ctgtggtaac ataaaagcca atgacaaaga tgacaataat
    22681 aagagcccag aagaaaggaa tatgaaagag aacttcaaag agccgtgcag tgcctgtgag
    22741 gacagcaaca gtatatgcta tcatcgaaaa ggcaacaaca atggccaaga caggcctcaa
    22801 ggcttcggtt ctatatcgct tcaccagcat ttccgtgact gtgaaggccc caaccttttc
    22861 ctgaagtttc ctcaccctct taccaaggag ggcccaaact agataggtca tgaaaagaag
    22921 gtttgatgca gcgacccaca tgagagaagc acccacatca tacgccttac ccccaaaacc
    22981 gataatcagg gtggcgctgt aacacgtggc gccgaaggcc agggccacag cccatgggcc
    23041 catagcatga cccgaagtgt aataactctc tgcgctcttg attttccgca taccgtaaag
    23101 accgacgccc acattcatca agagatatat gaccaggatg gtaatggcca tgggtatgta
    23161 gttttccata acaaggctct cctttgattt attgctgaga tccctcgggt gcgccccgtc
    23221 aaaggccctt tgcaggcgaa cgggttacag tgcagtatta attaaagata cattaaaacc
    23281 gctttgctcc cttagtcctc tatcttttta atatcccatg attcgctact ggcgataaat
    23341 ggatgaatga ttgataccac gaccgtggcc aaaccgatta tatatgcaaa aaaaactccc
    23401 cagctcaacc cgagcatgac caacctccta tttaatttga agcaaccagg gtgaatcgaa
    23461 aaggtgatgc ctgaaaagtt gcataacctt ttatgtgtag ccttggccat attatcatat
    23521 ttgggaactt ttcttgtcaa ggtttttatc aacccaaaac aattgtcctt ttttcttcga
    23581 cccttgcgta cataaaagaa gccggttttc tgaaagactc cgaggaaaaa gagaacctcg
    23641 ggaaagtatg ctgatccgct tgtgcccttg ccaggcatca caacaactgc tttcgaaggt
    23701 tttctgtgga ca
//
LOCUS       DAOOMC010000029        23682 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdus sp. isolate AMR_MDS_2685
            NODE_2034_length_23682_cov_6.356245, whole genome shotgun sequence.
ACCESSION   DAOOMC010000029 DAOOMC010000000
VERSION     DAOOMC010000029.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864760
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdus sp. (wastewater metagenome)
  ORGANISM  Syntrophorhabdus sp.
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae; Syntrophorhabdus.
REFERENCE   1  (bases 1 to 23682)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23682)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,729
            Genes (coding)                    :: 2,708
            CDSs (with protein)               :: 2,708
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 7 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23682
                     /organism="Syntrophorhabdus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2034_length_23682_cov_6.356245"
                     /isolate="AMR_MDS_2685"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2699756"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..379
                     /locus_tag="PK125_04480"
     CDS             <1..379
                     /locus_tag="PK125_04480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB37398.1"
                     /translation="NNLIEFLYARLCLRLEMIDEAIYTLNALESTGADFKGLHKAMAE
                     AYIHRGQMEKAVEKFRHAFPAEHVYIPFWCDNCHAKKVEWEDFCDNCYSWNTINVRKE
                     DFLRSDSAELQALYESEDWVEGA"
     gene            379..1578
                     /locus_tag="PK125_04485"
     CDS             379..1578
                     /locus_tag="PK125_04485"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00306.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HPB37399.1"
                     /translation="MMRHELILNNDNKLIFLILDGVGDIPNPQFSYTTPLEAAKKPNI
                     DSLAREKGILGRIIPVDIGITPGSGPGHLSLFGYDPLEHEIGRGVLEVLGLNMDLQDG
                     DLAARANFCTIRDNIVVDRRAGRIPTEETVRICEKISSAIPEIEGVKLLIKPGKSHRF
                     AVIFRGKNLSEHLTDADPHKDHLPVVFSEPKDDKAIFASHIVNTFIEKVAVLLKGEKA
                     ANGVLLRGFSAKPAIPSFSDNYGMKALAIATYPMYRGIAKVLGMDVKDETKDYQEMLK
                     ALRDNYDSYQFFFLHIKETDLAGEDGNFPQKVAAIETVDPMIPEIYSLNPQVLVITGD
                     HSTPCPMKGHSWHPVPLLLVTKTGERDGMAFHEKNCVTGSIGTIYSKQLMPLVLAHGL
                     RLDKYGA"
     gene            1559..2263
                     /locus_tag="PK125_04490"
     CDS             1559..2263
                     /locus_tag="PK125_04490"
                     /inference="COORDINATES: protein motif:HMM:NF039185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HPB37400.1"
                     /translation="MISTAHNLVKDLVNLICPLRCGGCNVHGHTLCPQCIDSFLVVDE
                     ETSCPICGMPVGIRKVCGRCIAEKNMFAEGLYGFYYENRLRDAMHSFKFSGRKDVGRC
                     LVGLVEEKISAFSDKFDCIIPIPVTEKRLRERGFNQSFILAEEIARMTGRPVLHSMLR
                     KTKETKDQYTLSREERRKNIKGAFSLTGNTGLKGKMVLLVDDLYTTGQTSREAARILY
                     QGKAQSVVLFALARTP"
     gene            2260..5319
                     /locus_tag="PK125_04495"
     CDS             2260..5319
                     /locus_tag="PK125_04495"
                     /inference="COORDINATES: protein motif:HMM:NF024893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA-like C-terminal domain-containing protein"
                     /protein_id="HPB37401.1"
                     /translation="MIKRLFYIAGGLVLAVILAVAIILVNISTIGPYIVKKITRGEVS
                     VKKFDYSFDKGMIVLKLVDLAITGNLEGTVKRIDVLANLTSRPFLKSTTISDFDLSFA
                     DLKGKIHLVPLPAELFQIKKGTVTYNKQKIFIEDLTIENLRSGKPFHFNLKAQNDSFF
                     KSISASGEGLYRGRSSELKGNVHITDLDLARVSGKLKGVAAVQGPFTFAKQSFTFEGP
                     FEMSGFEVKDRVLEKPLIVKHYSGKVKIAYADNITNITIGDIDFLNTTFLLHIKTGKN
                     SLFALDLSSGFIDINRVKSYMALENLAEGSKKLWDSIHKGSVKISKFQYEDKKSVHVD
                     LELKDVGFVYKDMNFIGVAGFLTIDNHKATISKGRGTFKTSHFSEAAGFVSLARDKMI
                     KVKGKYSVNLRDIPYMLDVGKVKFKHGITQGVMELEGNRTSGYRISGRGTVEHADVSW
                     QKISAKARGSYRFTHDEITFDPLILRKAGTDMVIRGKWGKKSMGIFMKGSLDVDQVRQ
                     FAVSPFPMEGLAFLDVSIQRHDNLFTLNGDVFMDNVSFNIPGFMKKDKGVKSAAHMAL
                     SVKDRRIDISRLSYNLDNLRISGKGWINPDRTVNADIGVNVQAVDRIAPLFFFEDHVP
                     KGDLDLNIAFRDLRWPLQTMPKMRGFARVNNGFLKLPWCAEPLKEITLNAEFKGDIFE
                     ILIKRVVCGQTTVGTSRLHIQDLENPRFNFDIAMENLDVSDIKSKSESIFRAIRQDSI
                     MARMAGDFTLHAGTVSLSNMSGTKLRVSGKLRDRKFSINQFTANVLGGYGDVTGMVDL
                     SQDTPVISVQGKILSMTSGHLLKTLGIEARAIEGEGVATGNIHFRGEKKTDFIQTLNG
                     QVGIYSKDGTIKKWNLLAKIFSLLNLYDLFRGKVRFTETGLKYTKMGASFKIKDGYLT
                     TDNFVLDSPSMLITGKGTINASDNEVNGIIAVSPLVAIDKTINKIPVLRRLVRDKNRG
                     FISASYNVKGKVTDPDISLNYVDTIGGKTINTLKNVITLPIELLERK"
     gene            5452..6333
                     /locus_tag="PK125_04500"
     CDS             5452..6333
                     /locus_tag="PK125_04500"
                     /inference="COORDINATES: protein motif:HMM:NF022188.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2520 domain-containing protein"
                     /protein_id="HPB37402.1"
                     /translation="MGIRGMKIGFIGTGKVGIAIAHTLKNKGFDIVAVSDNTGEKAFN
                     TARPYLGESIRYTNSNMDVVGLSDVIAITTQDYAISEVAKEIYDKARRLDNKLFFHTS
                     GAHPSSILKPLDEKGAVIGSLHPLQSFPDIDSAIRVLPDTYVFIEGSADALAVLETLG
                     KNIGQTTVSIEGNNKVYYHLCAVFVCNLLCALLYASEKIMQRIDITLEPFFPIINATL
                     HNIQKNGPLVSLTGPIVRGDRETVQDHIHAMGNMGLHRRIYKALSLTALDMVKERGIH
                     NQETITVLQRILEAIDE"
     gene            6326..7060
                     /locus_tag="PK125_04505"
     CDS             6326..7060
                     /locus_tag="PK125_04505"
                     /inference="COORDINATES: protein motif:HMM:NF001753.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent deacylase"
                     /protein_id="HPB37403.1"
                     /translation="MNESVVERLTQANSLLVITGAGISAESGIPTFRGADGLWRNYRA
                     EDLATLDAFERDPVTVWEWYDWRRGIIGRAEPNPGHLAIKDLEDMFENFLLITQNVDG
                     LHGRTGIRNIVEIHGNLWRTKCTREGKISMLMDVPLRSIPPRCDCGAVLRPDVVWFGE
                     SIPSYALDLSFNVLEQCDTLIVVGTSGVVYPVASFPQTVKDNGGYVVEVNVEPTPISA
                     IADASLYGNSGDILPMLVKWLKERRK"
     gene            complement(7153..7677)
                     /locus_tag="PK125_04510"
     CDS             complement(7153..7677)
                     /locus_tag="PK125_04510"
                     /inference="COORDINATES: protein motif:HMM:NF039354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6125 family protein"
                     /protein_id="HPB37404.1"
                     /translation="MNIGVLETMEAPELREYLRFLLWHYRVMDGFWFLFVEKEYGRKA
                     AEYLDELVWAKIAGMSAKDLVQRFNIQEKGIKGLVKALQFLPWTMLVGYHVSQTGEDE
                     ALVTVPECPTQMARVKHGLPEFHCQDMHQAEFESFAHAIDPRIKVECLLAPPDHPPDM
                     FCKWRFTIDESKVV"
     gene            8099..8344
                     /locus_tag="PK125_04515"
     CDS             8099..8344
                     /locus_tag="PK125_04515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB37405.1"
                     /translation="MKTTIQIPDVHFEEVRMLARQENTTVKVIIEEGLRPIISERKRR
                     SYFKLRKVTFKGTDFQPHLAGTIWDQILQVSYEGRGA"
     gene            complement(8987..9571)
                     /locus_tag="PK125_04520"
     CDS             complement(8987..9571)
                     /locus_tag="PK125_04520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB37406.1"
                     /translation="MNWQRGLMLLLIVAVIGGCATMPAGPTVRVMPGPGKPFEVFIDE
                     DTMCRQWALQQIGGLPPGETANQNLASGAVLGTLLGAGIGAAIGSTTGDAGAGAAIGA
                     GAGLLGGTAVASNSAYASQWELQRQYDIAYSQCMYAKGNQVPGVVKRQPQADVPPPPP
                     PPSVNPGSWVTVPGQYVNGKWVPEHKVFVPPPAR"
     gene            complement(9568..10110)
                     /locus_tag="PK125_04525"
     CDS             complement(9568..10110)
                     /locus_tag="PK125_04525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB37407.1"
                     /translation="MKKIIVIVVIGVFFSFGVFSEGFCARGGPPGGGSSGGRPPGGGA
                     ISGPRPGDGHRGGGYYGGRYHRGGGYHYYRGGSRYYFGWGLGFPFYPYPFDYYASGYP
                     YVYPYWYPYYPYAYPDYRTAFPDYPYSDDQAAVYVEPEQPYYWYYCSNPEGYYPYVTS
                     CPGGWAKVVPTPQGEEGKVK"
     gene            complement(10202..10483)
                     /locus_tag="PK125_04530"
     CDS             complement(10202..10483)
                     /locus_tag="PK125_04530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB37408.1"
                     /translation="MSLLPAMIVMTPVTGLRILYLLTINIPQAGIPQQDLLMLPHHPV
                     QTTMDTLITGLNILIITGMDITGLDIPATMATGITGPGINRLGFQCCSK"
     gene            11072..11758
                     /locus_tag="PK125_04535"
     CDS             11072..11758
                     /locus_tag="PK125_04535"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HPB37409.1"
                     /translation="MRTRQIVENVYQVGGAAISDVRDGAVYLLDFDELVLIDSGAGLG
                     FEKTIRNIETIGFYPSKISTVILTHCHMDHIGGATLFRQHCGSRIVMHALDAEIIERG
                     DQHMTAAWCFGIYLQPFRIDVKLRGNQGRLVFGEHAVNWIHIPGHTPGSIALYLGEGG
                     RRILFAQDMSAPLLEDFKCDPIAWRESMDKLLALGADILCDSHAGVFRPKHRVKAYIE
                     RSIIEHGFQS"
     gene            complement(11966..12121)
                     /locus_tag="PK125_04540"
     CDS             complement(11966..12121)
                     /locus_tag="PK125_04540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB37410.1"
                     /translation="MRLQQERWLEFSPPVFEEVADPGPIIVAGAFCFSMGMTITGGEV
                     LHSYINE"
     gene            complement(12316..13659)
                     /locus_tag="PK125_04545"
     CDS             complement(12316..13659)
                     /locus_tag="PK125_04545"
                     /inference="COORDINATES: protein motif:HMM:NF014135.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HPB37411.1"
                     /translation="MSELQIVTTYAVFAAVILAIAFNILDLALAGLLGVSVLIVFGIL
                     TQGDIINVVKMSGSTFALLFGGMIVARTLTTSGIFEFLGALFLRKTRGRGKRFLLGLV
                     ILVAIPCMFLPNATTVLLLAPIIIRVAAVLKIDIIAPLVLTAIISNSAGLLTLVGDPA
                     TFLVGTSINITFIEYFKRVSAGGLVSLLVLIPLLPLIMKDIWRIELTLPDDLKGETFK
                     RPWLSVLSLGVLGIMVLLFLFGEFLPTSIVPASASIIACSVALLIIYGAKVEPVANVL
                     RDIDWKTIIFLACMFILVEAFTKTGALRGLSSHMSQWFGSNLVPLAFALIVGVGFASS
                     LLANIPIVATMILLVKGYLISTEFVPEDALGVFFTDWPPVTLPVFVAMMFGGTLGGNA
                     TLIGASANVVSAGISAAHGRPITFAAFLRYGLPITVCQLAASALYVWILSMVVRS"
     gene            complement(13711..14736)
                     /locus_tag="PK125_04550"
     CDS             complement(13711..14736)
                     /locus_tag="PK125_04550"
                     /inference="COORDINATES: protein motif:HMM:NF012707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HPB37412.1"
                     /translation="MARLQRFIILFIVILLFPSIAGSYETLKKVRSKGVLRCGVSEGL
                     PGFSMKDAKGQWTGMDADFCRAVAAAVFGDPEKVTFYPLTASERLPALRTGEIDLLSR
                     NTTWTIGRETMLGLQFVGTLYYDGQGFLVRKSSGITKVSHLKNAAICIEKGTTSQANL
                     DDYFRTKGWRYKPVIFESIIKAQEAFFSGQCKSYTSDRSHLAAVRSGVRDGSKTYAIL
                     PGQISKEPLGPVVRRGDDEWFTLVRWVLFALIEAEEIGVTQANVRAKAKEKGNPDLER
                     FLGSSGNFGKSLGIANDWTVRIVESVGNYGEMFERNLGRGSPLNLDRGPNRLWNKGGL
                     MYAPPFR"
     gene            complement(14809..16914)
                     /gene="glgX"
                     /locus_tag="PK125_04555"
     CDS             complement(14809..16914)
                     /gene="glgX"
                     /locus_tag="PK125_04555"
                     /inference="COORDINATES: protein motif:HMM:TIGR02100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004133 - glycogen debranching enzyme
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HPB37413.1"
                     /translation="MSFSTERNARTYEIGPGNRYPLGATFDGEGVNFAIWCWAATRVE
                     LLLFECADSPHPFQIITLDDVINRTFFCWHVYVAGLTPDICYGWRTDGPGNTPRTGHR
                     FDREKILLDPVARAVTDLLWNRERAIQPGDNCDASMRAILVEEDMYDWEGDKPLERPV
                     EETIIYEMHVRGFTRHPNSGVINPGTFAGVIEKIPYLKDLGITDVELMPTMAFDEQCV
                     PEGTAALGLKNYWGYCTHSFFAPHPGYSVTPHLGTQIREFRDMVKALHRAGIGVIMDV
                     AFNHTAEGGGEGPTINFKGFGNSGFYHLYPHDRRLYRDFTGCGNTVNCNHPVISRFII
                     DCLEYWVREMHIDGFRFDLASVFARDMDGIPKRFAPTPWLIELSQTLANTKIIAEAWD
                     AAGLYQVGGYPGYRWAEWNGMYRDIIRRFVRGEKGIIGEVATRLMGSSDLYGREKRLP
                     SNSINFITCHDGFTVHDLVSYNEKHNHSNGDDNQDGSNDNLSWNCGVEGETNDERITS
                     LRQRQTRNFMAILLLSQGIPMLLYGDEVLRTQKGNNNAYCQDNEISWFDWSLTSRNRD
                     MLRFVREMIAFRKRHPCLMHQRFLKGTREPGSTYPDVTWHGVKLYEPAWEDPDAQVLA
                     FTLGAVRVDEEDLHIVLNMSEESLSIPLPDPAGRLWYCAIDTAKPSPDDIAEPGKQVG
                     QPYGYCRVSPRSVVVFERR"
     gene            complement(16921..18225)
                     /locus_tag="PK125_04560"
     CDS             complement(16921..18225)
                     /locus_tag="PK125_04560"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: protein motif:HMM:NF005301.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent 6-phosphofructokinase"
                     /protein_id="HPB37414.1"
                     /translation="MEDELDLNIEKLGECRIPSPVSIGRFTKDNENVLFRVTLPDIET
                     YLASGRRPPAFEQAGPREKIYFDPSKLKCGIVTCGGLCPGLNDVIRSIVLSLYYHYGV
                     RTVFGFPYGYEGLSYRHGHTPLDLTPKSVEGIHEKGGTILGTSRGPQDISEMVDTLDR
                     MNIGVLFVIGGDGTLKGAKAISEEIKNRGLKISVIGIPKTIDNDISYVQKSFGFETAV
                     AEAKKAIFSAHTEALGARNGIGLVKLMGRESGHIASYATLACNDVNFCLIPESLFTME
                     GFLHALKKRLQERGHAVIVVAEGAGQNFLKDTGERDASGNIRFADIGHYLRDMIRNFF
                     KKSTMQISMKYIDPSYTIRSVPANASDSAFALILGQNAVHAAMTGRTNMLVGTWMNRY
                     THVPISLAVSRRKKIDPDGWIWASVLTATGQPRNMVDPVSPP"
     gene            18402..18842
                     /locus_tag="PK125_04565"
     CDS             18402..18842
                     /locus_tag="PK125_04565"
                     /inference="COORDINATES: protein motif:HMM:NF024634.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HPB37415.1"
                     /translation="MKILTTPRMDRCIGCHSCSLACARLVHRRLSWDTAGIRIASSGG
                     LSTGFEARTCLACDPAPCVKACPTGAYTQRKGGGVVVKISLCNRCGACARACPVDAIF
                     LDNTGQPFVCIHCGRCISFCPHACLEMGDVAAQPMEKGEEEVNL"
     gene            18839..20587
                     /locus_tag="PK125_04570"
     CDS             18839..20587
                     /locus_tag="PK125_04570"
                     /inference="COORDINATES: protein motif:HMM:NF013481.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016625 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, iron-sulfur
                     protein as acceptor [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase N-terminal
                     domain-containing protein"
                     /protein_id="HPB37416.1"
                     /translation="MISNQFKILKIDLSSGKGTYEFIEGRDAFAGGSGLAALLFNLYG
                     HTDRPWDDPDQPLIFTIGPLTGYFPLMSKTVCAFKSPYHNQYAESHGGGRSALALRFA
                     DVDGLVITGQANRLSCLSISSSHFQVKEAHYMKGMDVFRTGRMLRHMFGNSGHRSILR
                     IGPAGERGAPTACINVDTYRHFGRLGGGGVMGSKNLKAIVIIGDKVFDLPTGDSYGKL
                     YQNVYRKMTATEIMHKYHDIGTPVNVASLNEVMSLPIRNLKQTTDPEMAGITGERFAS
                     DALLRNHACSGCPVGCIHVGYVRERFAKENRYFYLQVSYDHEPVFAVGSMLGITNCFS
                     VLGIIDAVEKVGLDVMSAGVCLAWATEATEKGLISEKETLVPLGFGDASTYRQAVHHL
                     GVGTNDFYRVLSQGINEAVKHFGGSDFSCVLGQEMAGYATGEVFFVSQALGFRHSHLD
                     SAGYSYDQKTTEKDVDRAVEFLVQDEKGRVLLTSMVSCLFARNVYTDEVVSDCLKSLG
                     YNGLAVKLESVALNIQQLRWRLRFASGFNPDDVIISKRFGEVTTVKGPIDMFYLDALK
                     KEYGKRIKAMALAAPP"
     gene            complement(20562..21560)
                     /locus_tag="PK125_04575"
     CDS             complement(20562..21560)
                     /locus_tag="PK125_04575"
                     /inference="COORDINATES: protein motif:HMM:NF024779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4105 domain-containing protein"
                     /protein_id="HPB37417.1"
                     /translation="MGKTVRFFVILCLSIIIACMTLWAALAIFYSNLAEETLRTNIAI
                     GFAVGTAAAFLFIKKRLQVLIGYLIVFCLIVAWWMNIKPSNDRDWQPDVAVLPYATIQ
                     DNQITIHNIRNLTYRTETDFDSHYYDKTFDLKALDSVDLIAVYWMGDAIAHIMMSFGF
                     QEEDFVVFSIETRKEKGEGYSTIKGFFKQYELIYIAGDERDLIRVRTDYRNPQEDVYL
                     YRLNINRERAEKLFMAYIRQINEMKKRPVWYNTLTTNCTTNIVMHIKTFSDRIHYSWK
                     ILLSGYGPQYAYERGTLDSNIPFAELRQRSYINPRAHVIGNDPEFSRKIRVGLPRP"
     gene            complement(21566..23458)
                     /locus_tag="PK125_04580"
     CDS             complement(21566..23458)
                     /locus_tag="PK125_04580"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HPB37418.1"
                     /translation="MMKRCRDRIKYPKYVAYIGLLVFIAMFLGGCATPVGVRQLDPKQ
                     VQRNLTASILSSDELSAYTQQILNRADLSARFRTQPAEVLAALHKGLPTASEADRFFA
                     LAELSYAYASKGGPQSYYSASVVYAYAFLFPKISRTDPDPTDPRVRIALDLYNRSLAQ
                     AFTAGDRAKVMIGEGNYPLPFGELIVKVDAEEFLWGSFRLTDFVQAAEFDVRGLRNRY
                     RWPGIGAPLIAATEPLKGVIVPEYSRIPPRIRVAATAFLRIDNVEEGIEAGKVTANLE
                     LYTTGEATSVDIGGRTVPLEYELSSAMAYTLEGSQVYRFELRGLFSGDFSLIGKKARY
                     QDGIFLMEPYRPGRIPVIFIHGTASSPARWAEMFNELSNDPVLWGNYQFWLFTYNTGN
                     PITYSAGILADGLRKTVDEMDPEGKDPALRRMVVIGHSQGGLLTKLTTVDSGLKFWTT
                     ALPFDEVNASAETKEILRRSVFFKPVPSVKRVVFIATPHGGSFVAGGWIGRLTSKFIS
                     LPFRLADTMKEVITMNPQLKAVRFIKDAPRSTDNMDPKSPFIKNLSSMPIAEGVTAHS
                     IIAVKNPKEPKDRWNDGVVEYTSAHIDGVASELVVNSGHSTQDHPETIEEVRRILLQH
                     LRESGE"
BASE COUNT         6421 a         5723 c         5534 g         6004 t
ORIGIN      
        1 aaataatctt atagagtttc tctatgcgcg gctttgcctc cgtcttgaaa tgattgatga
       61 agcaatctat acgctaaacg ccctcgagtc aacgggggca gattttaaag gccttcacaa
      121 ggccatggcc gaggcatata tccatcgggg acagatggag aaagctgttg aaaaattcag
      181 acacgcattc cctgccgaac atgtttacat acccttctgg tgcgataact gccatgcaaa
      241 aaaggtggag tgggaagatt tttgtgataa ttgctacagc tggaatacaa ttaatgtccg
      301 aaaggaagat ttccttcgga gtgactctgc tgaattgcaa gccctctacg aaagcgaaga
      361 ttgggttgaa ggagcgtaat gatgagacac gaacttatct taaataacga taacaagctc
      421 attttcctca ttcttgatgg tgtgggggat atccccaacc ctcagttttc ttataccaca
      481 ccccttgaag ctgccaaaaa accaaatatt gacagcttgg ccagagaaaa aggcatcctt
      541 ggacggatca tacctgttga catcggaata acaccgggca gcggaccggg acatctgagt
      601 ctcttcggtt atgatcctct tgaacatgag ataggcaggg gagttctgga agtccttggc
      661 cttaatatgg acctgcagga tggggatctt gctgcaaggg ccaatttctg cacgatcagg
      721 gacaacattg ttgttgacag acgggcagga agaataccaa cagaggaaac agtacggatc
      781 tgtgaaaaga taagcagtgc tatcccagaa attgaaggcg taaagcttct tatcaagcca
      841 gggaaatccc acaggtttgc agtcattttc cgggggaaaa atctttcaga gcacctgaca
      901 gatgctgatc ctcacaaaga tcatttgccc gtagtcttct ctgaaccgaa agatgacaag
      961 gccatttttg cttcacacat cgttaacact tttatagaaa aggtggctgt tcttctcaaa
     1021 ggtgaaaagg ctgcaaacgg tgttcttctg agaggctttt cagcgaaacc agcaatacca
     1081 tccttctcgg acaattacgg gatgaaggca cttgctattg ccacctatcc catgtacaga
     1141 ggaattgcaa aagtcctcgg tatggatgtg aaggatgaaa cgaaggacta ccaggagatg
     1201 ctgaaggcac tgagagacaa ctatgactcc tatcaatttt tctttcttca cataaaagaa
     1261 acggatcttg caggtgaaga cggtaacttc ccgcaaaagg ttgctgccat agaaaccgtt
     1321 gaccccatga tccccgagat ctatagcctc aatcctcaag tccttgtcat taccggagac
     1381 cattccacac cctgccctat gaagggtcat agctggcacc ctgttccgct gttgctggta
     1441 acaaaaacag gtgaaaggga tggaatggcc ttccatgaaa agaactgcgt taccggaagc
     1501 ataggaacaa tttacagtaa gcagctaatg ccccttgttc ttgcccacgg gctgagactt
     1561 gataagtacg gcgcataatc ttgtaaaaga ccttgtgaac ctgatctgtc ccctgcgttg
     1621 tggagggtgt aatgtccatg ggcataccct gtgtccgcaa tgcatcgatt cctttctcgt
     1681 tgttgacgaa gaaacatcct gtcccatatg tggaatgcct gtggggatac ggaaagtctg
     1741 cggaaggtgt attgctgaaa aaaacatgtt tgctgagggc ctttacggat tctactatga
     1801 gaacagactc cgagatgcta tgcactcgtt caagttctca ggacgaaaag atgttgggag
     1861 gtgtcttgta ggtcttgtcg aagaaaaaat ttctgccttt tcagacaaat ttgactgtat
     1921 cataccaatc cctgtcacag aaaaaagatt gcgagaaaga gggttcaacc agtcatttat
     1981 tcttgccgag gaaatagcaa ggatgaccgg cagacccgtt ctgcattcaa tgcttcgaaa
     2041 aacaaaagag accaaggatc agtacaccct ctcaagggag gagagaagga aaaacataaa
     2101 gggggctttc tctcttaccg gcaatacagg ccttaaaggg aaaatggtgc ttctcgttga
     2161 tgatctttac acaaccggtc aaacctcacg ggaagctgct cgcatccttt atcagggaaa
     2221 ggctcagagc gttgtgctct ttgccctcgc acggactcca tgatcaagag actattttac
     2281 attgcaggcg ggttggtcct tgcagttata ttggcagtcg ctattatcct tgtaaacatc
     2341 tccaccattg gcccttacat agttaaaaag attacccgtg gcgaagtctc tgtaaaaaaa
     2401 ttcgattaca gctttgataa ggggatgatc gttctcaaat tagttgactt agcaattaca
     2461 ggtaatttgg aaggaacggt aaaaagaatt gatgtcctgg caaacctgac gagtaggccc
     2521 ttcttgaaga gtaccactat ttcagatttt gacctgtctt ttgctgacct gaaaggcaaa
     2581 atacacctgg tgccgcttcc tgcagaactt ttccaaatca aaaagggcac agttacgtac
     2641 aataaacaga aaatattcat tgaagacttg acaattgaga acctccgatc cggaaaacca
     2701 ttccatttca acctcaaagc tcagaacgat tcctttttca aaagtatcag cgcctcaggt
     2761 gaaggcctct acaggggaag atcttccgaa ttgaagggca atgtacacat taccgacctt
     2821 gaccttgcca gggtgtcggg taaactcaag ggtgttgcag ctgttcaagg cccgttcacc
     2881 tttgccaaac aatcctttac cttcgagggg ccttttgaga tgtcaggatt tgaggtgaag
     2941 gaccgtgtcc ttgaaaaacc attaatagta aagcattact caggaaaggt aaagatcgca
     3001 tatgctgaca acataacgaa catcacgata ggggacattg actttctcaa tacaacattt
     3061 cttcttcata ttaaaacggg aaaaaacagt ctcttcgcgt tagacctctc ctccggcttc
     3121 attgacatca atagagtcaa gagctatatg gcccttgaaa atcttgcaga agggtcaaag
     3181 aagttgtggg attccataca caaggggagt gtaaaaattt caaagtttca atatgaagat
     3241 aaaaaatctg ttcacgtcga tcttgagctg aaagatgtag ggtttgtcta caaagacatg
     3301 aattttatag gagtagcagg ttttctcact attgacaatc ataaagcgac gatctctaaa
     3361 ggtcgtggaa ccttcaagac aagccatttc tcagaagcag caggtttcgt atcccttgcc
     3421 agagacaaga tgataaaggt aaaaggaaaa tactcggtta acctgagaga cattccgtat
     3481 atgcttgacg tgggaaaagt aaagttcaag catgggatta cccagggggt tatggaactg
     3541 gaaggcaaca gaacatcggg atacaggatt tccggaagag gcaccgtaga acatgctgat
     3601 gtgtcctggc agaaaatatc agccaaggcc aggggctcct atcgatttac ccatgacgaa
     3661 atcacctttg atcccctgat actcaggaag gccggcacag acatggtcat caggggaaag
     3721 tggggcaaga agtccatggg cattttcatg aaggggagcc ttgatgtgga tcaggtcaga
     3781 caatttgctg tcagtccatt tccaatggag gggctggcat ttcttgacgt atcaattcag
     3841 agacacgaca atcttttcac actgaatgga gatgtgttca tggacaacgt ctcctttaac
     3901 attccggggt tcatgaaaaa agataaagga gttaaaagtg cagcccacat ggctctgtca
     3961 gtgaaagaca gacgtattga tatcagccgt ctttcctaca accttgacaa cctgcgtata
     4021 agcggaaaag ggtggattaa ccctgacaga actgtgaatg cagatatagg cgtgaacgtt
     4081 caagcagttg accggattgc cccactcttt ttttttgagg accatgtgcc gaagggcgat
     4141 cttgatctca acattgcttt cagagatctg agatggcctt tgcaaacgat gcccaagatg
     4201 agaggctttg cgagggttaa taatggcttc ctgaagcttc cctggtgcgc cgaaccttta
     4261 aaagaaataa ctctgaacgc ggaattcaag ggagacatct ttgaaatact gataaaaaga
     4321 gtagtatgtg ggcaaaccac agtgggtacc agcaggcttc acattcagga tctggagaac
     4381 ccaagattca atttcgatat tgccatggaa aaccttgatg tgagcgacat caaaagcaag
     4441 tcagaatcta tatttcgcgc tatccgtcag gacagtataa tggcgagaat ggctggtgac
     4501 tttacccttc acgcaggaac tgtaagcctg tcaaatatgt ctggaacaaa actcagggtc
     4561 tcaggaaaac tccgtgacag gaagtttagc atcaatcagt tcaccgccaa tgtccttgga
     4621 ggttatgggg atgttacagg catggtagat ctttcccagg ataccccggt cataagtgtc
     4681 caaggcaaga tcctgtcaat gacaagcggc catttactga agacccttgg tatagaggca
     4741 agagccattg agggagaagg ggttgctact ggcaatattc atttcagagg agagaagaag
     4801 accgacttta tccagaccct caatggacag gtaggcattt acagcaaaga tggtacaatc
     4861 aaaaaatgga accttcttgc aaagattttt agccttctca atctttacga tctctttcga
     4921 ggcaaagtcc gattcactga gactgggctt aagtacacaa agatgggcgc ttcatttaag
     4981 ataaaagacg gctatcttac gaccgataat tttgtcctcg atagtccctc aatgctcata
     5041 acaggaaaag gcacgatcaa tgccagtgac aacgaagtaa atggcattat cgcagtatcg
     5101 cctctcgtgg ctatcgacaa aacaattaac aaaatccctg tccttcgaag acttgtacga
     5161 gataaaaaca ggggtttcat ttccgcttca tacaatgtaa agggcaaggt cacagatcca
     5221 gacataagcc tgaactacgt tgatactatc gggggaaaaa caattaatac attgaagaat
     5281 gtgatcacct tgccaataga attgttagaa aggaaataga tgggctaatg ggtagagcgg
     5341 gcaagaggcc gttggctata tgtgggaaaa aatgcaatat tgatcgaatg aaacccagtt
     5401 tctctggttt tgtaacgata gaaatttcat aaacatatga aaggaaaact gatgggaatc
     5461 agaggtatga agattggatt cataggaact ggcaaagtgg gcattgcaat tgctcacaca
     5521 cttaaaaata agggatttga cattgttgcc gtttcagaca ataccggaga gaaagctttc
     5581 aacacagcac ggccatatct tggagaatca ataagataca caaacagcaa catggatgtg
     5641 gtcggattgt cagatgttat tgccattacc acccaggatt acgcaatcag cgaggtcgcc
     5701 aaagagattt atgacaaagc caggcgcctg gataacaaac tctttttcca caccagcggt
     5761 gctcatcctt catcgatact gaaacccctt gacgagaaag gtgctgttat cgggtctcta
     5821 caccctctcc aatcatttcc tgacattgat agtgccattc gtgtcctccc agatacttac
     5881 gttttcatag agggcagcgc agatgccttg gctgtccttg agaccctcgg aaaaaatatt
     5941 ggccaaacca cagtctctat cgaagggaac aataaggtct attaccacct ctgcgcagtc
     6001 tttgtatgca acctgctctg cgccctgctc tatgcaagtg agaaaattat gcaaaggatt
     6061 gatataaccc ttgaaccttt tttccccatc ataaacgcca cgttgcacaa tatccagaaa
     6121 aacgggcccc tggtttccct gacaggacca atcgttcgcg gagacaggga aacagttcag
     6181 gatcacatac atgcaatggg aaacatgggg cttcacagaa gaatctacaa ggctctatct
     6241 ttaacagccc ttgatatggt aaaagaaaga ggaatacaca atcaggaaac cattacggtt
     6301 ttgcagagaa tactggaggc gatagatgaa tgagagcgtt gtggagaggc taacccaggc
     6361 aaattctctc cttgtcatta caggggcagg tatctcagca gagagtggca tacccacctt
     6421 taggggtgcg gatggactat ggagaaatta ccgcgctgaa gaccttgcaa ccctggatgc
     6481 ttttgaacgg gatccggtaa cagtgtggga atggtacgac tggagacgag gcattatcgg
     6541 cagggcagaa ccaaatcccg gccacctcgc cataaaagac ctcgaagaca tgtttgaaaa
     6601 ctttcttctt ataacccaga atgtggatgg cctccacggg aggacgggaa taaggaacat
     6661 tgtagagatt catggcaatc tatggcggac aaagtgtaca cgagaaggca aaatctccat
     6721 gctcatggat gtacccctca gatcgatccc accaaggtgc gactgtgggg cggttttaag
     6781 acctgacgtc gtgtggtttg gcgaatctat accttcatat gccctcgacc tgtccttcaa
     6841 tgtcctggaa caatgtgaca cgctcatcgt ggtgggcaca tcaggggttg tgtaccctgt
     6901 ggcatctttc ccccagacag tcaaagacaa tggtggctat gtggtggaag tcaatgtgga
     6961 gcccacccct atcagcgcta tcgctgacgc atccctttac ggaaactctg gcgacatcct
     7021 gcccatgctg gtcaaatggc ttaaggaaag gaggaaataa aggtccgaaa taccaaacaa
     7081 atcttcacga cccttctcat cccccgaagg actccacgtt ctctgcactt cagcgagaga
     7141 tagctttctg tatcaaacca ccttggattc gtcgattgta aatctccact tgcagaacat
     7201 gtccggtgga tgatcgggcg gtgcaagaag gcattcaacc ttgatcctcg gatcaatggc
     7261 atgagcaaat gactcaaatt cagcctggtg catatcctga cagtggaatt ccggtagacc
     7321 gtgtttcacc cgtgccatct gggtaggaca ctccggcaca gtgacaagcg cctcatcctc
     7381 tccagtctgg cttacatgat acccaacaag cattgtccac ggaaggaatt gaagggcctt
     7441 taccagcccc ttgatacctt tttcctgtat gttgaatcgc tggacaaggt ctttggcaga
     7501 catcccggca atctttgccc acacaagctc atcaagatac tcggcagcct tccttccata
     7561 ttccttctca acaaacaaga accagaaacc gtccattacg cggtagtgcc ataggagaaa
     7621 ccgcaggtac tcacgaagct caggagcctc cattgtttcc aaaacgccta tgttcattcc
     7681 aacctcctat tacatcagtt gcacgttacg gccacgagtg tcaatctgca gcatgaaaaa
     7741 aaccgatatc tgaagactgt gcagtgctgg tttcggcata ttctatcaat cccgtaacca
     7801 gcctgtcaat agagccatgg agccgttcag ctcctttcca tcacgaactt ctacgtcagg
     7861 aatcttaaga cagcattcag tcctcatatt ttctattgta aaaccttatt tcagatccac
     7921 cctgcagttc agttcaacac aaaacaagtt tcattcccca tgcagaaatc atcttaactc
     7981 aactaatttt gttttcagtg agaagggtga tgcaaaacaa aaaaaggtaa aaacagagtt
     8041 gtaagaaaag acagaatctg cattacggtt taccacatag tgtgatatgg ttatccatat
     8101 gaagacaacc atacaaatac cggatgttca ctttgaggaa gtgcggatgt tagcccgcca
     8161 ggagaatact actgtgaagg ttattattga agagggtctt cgtcctatta tatctgaacg
     8221 gaaacgtcga agctatttca agctgagaaa agttacattc aagggcacag attttcagcc
     8281 gcaccttgcc ggaacaatct gggaccaaat ccttcaggta agttacgaag gacggggcgc
     8341 ttgattgcgg tgaacagcaa cattctgttt tatgctcatc gggaagactc gccctgacat
     8401 ggtcaagccc tcagaaggat tgttgaattg gctgagggca gagccccgtg ggccataccg
     8461 tggccatgta tccacgaatt tttcgcgata gtaacccacc ctcgaatata taaccctccg
     8521 acgccgcttg agacagcaat tgaccaagtt gaggcatggc ttgagtcacc cgccctcgcc
     8581 atgctttcgg aatctgaaga ttactggcgg ttgttacgtt ccacccttca aagtggccgc
     8641 atttcagatc cccaggtaca cgacgcccgc atagtaacaa tatgccacca acatggggta
     8701 agggagttat ggacagctga cagagacttt ggtcgctttc cgggacttgc tgtccaaaat
     8761 ccactgattt cttgacaagg tcagtttggt aaaatctgcg tgagaaacgg tcttactcag
     8821 tctgccggct tgatgccggc tttttctgcc aggcagacaa gatcgtccgt aaaggacatg
     8881 tgaaccgtct caattagtca cctattcccg attgcatact gaacaccagg tctttatgga
     8941 gtcggggacc tggccaggac attggctttc tcgggggtag aacctgctat ctggcaggag
     9001 gcggtacgaa cactttatgt tcaggtaccc acttaccatt cacatattgg ccaggtacgg
     9061 taacccatga tcctggattt acactcggcg gtggtggagg tggcggcaca tcagcctggg
     9121 gctggcgttt aaccacaccc ggaacctggt tgcctttcgc gtacatgcat tgcgaataag
     9181 ctatatcgta ctgcctttgg agctcccact gggaagcata agcagaattc gatgccactg
     9241 cggttccccc gagaagaccg gcccctgcac ctattgcagc acctgcgcca gcatcaccgg
     9301 tggtagatcc aatagcagca cctatgcctg cacccagaag cgttccaagg accgctccag
     9361 aagccagatt ttggttagca gtctcgcctg gtggcaatcc tccaatctgc tgcagagccc
     9421 attgcctgca cattgtatct tcatcaataa agacctcgaa aggttttcca ggacccggca
     9481 tcaccctcac agtcgggcct gccggcatgg ttgcgcaccc gcctatcact gccacgatta
     9541 ataacagcat taaacctcgt tgccaattca ttttacctta ccctcctctc cttgcggtgt
     9601 aggaactacc ttggcccatc cacccggaca actggtgaca tagggataat acccctctgg
     9661 attgctgcag taataccaat aataaggctg ctcaggctcg acgtagactg cagcttgatc
     9721 atcagaatat ggatagtccg gaaacgcagt gcgatagtct ggataggcat agggataata
     9781 cggataccaa tagggatata cataggggta gccagatgca taatagtcaa aggggtatgg
     9841 gtagaacggg aatcccaggc cccaaccgaa atagtatctt gaaccgccgc gatagtaatg
     9901 ataaccaccg ccacgatgat atctacctcc gtaataacca ccaccgcgat ggccgtcacc
     9961 aggacgtgga ccacttatag cgccccctcc aggaggacgt ccgcctgaag agccgccgcc
    10021 aggaggacca ccgcgtgcgc agaagccttc tgagaaaaca ccaaaagaga aaaacactcc
    10081 gataacgacg ataacaatga ttttcttcat attttgcctt cctttcccaa acataacctc
    10141 aaaaggttct tgtgtcaata tcttcatctt aaccgaaaaa cctcgccctt attctgcatc
    10201 tttacttgct gcagcactga aaacccaagc gattaatacc ggggccggta atacctgtag
    10261 ccatagtagc cgggatatcg aggcccgtaa tatccatgcc cgtaataatc aggatattga
    10321 gtcctgtaat aagggtatcc atagtagttt gtaccggatg atgagggagc atcagcaagt
    10381 cctgttgtgg gatacctgcc tgaggaatat tgattgtcag aagatacaga atacgaagac
    10441 ccgtaacagg agtcataaca atcatagcag gcaagaggga caattgcaca ccctgtcaac
    10501 acacttagcc caaggacaat cacaatgatt ttcaccgggt acctccttca gccttgcggt
    10561 tttactgcaa catttttcat tataaaaccg acaacctatc gtgttctttg tctttcaaaa
    10621 cacttatagc aatacttatg cctcagctac ttgattgaaa gaagctgaaa taattggata
    10681 aaccctcaat ctacccgatt gcttcttaca tttttgtcgt tttgaagaaa aatatccgac
    10741 aaaaccgtcg agggttttag aggcaaaaaa ttaaggctga tccagggtgc aattattatc
    10801 tatctatggc gcgtggtatg ggcatatcta tcgcctcttg cttcttcata acccttcatg
    10861 cattgactgg atgctcgatt gactataata gttataacaa aatgactgaa aaatatggta
    10921 tgaaaacaac agctatctct gaattacaaa tgcactttag tgaatattta agctatgtga
    10981 aaagcggtga tgaaggatgg ttacagaagg aaggtttaac gcttttttca tagcacaatt
    11041 tgacatcttc ttttgagagt atattctaaa gatgagaaca cgtcagatag ttgaaaatgt
    11101 ttaccaggtt ggcggcgctg ccatttctga tgtcagggat ggggcggttt accttctgga
    11161 ctttgatgaa ctcgtcctta tcgacagtgg ggccgggctt ggctttgaaa aaactatcag
    11221 aaacattgag accattggat tctatccttc caaaatatca accgtcatct tgactcactg
    11281 ccacatggat catataggag gcgcgacact gttcaggcaa cactgtggga gcaggattgt
    11341 tatgcatgca ttggatgctg agatcataga gcggggggac cagcatatga ccgctgcatg
    11401 gtgctttggc atttacctcc agccattccg aatcgatgta aaactccggg gcaaccaagg
    11461 acgtctcgtt tttggagagc atgcggtcaa ctggatccac atacccgggc acacaccggg
    11521 atcaattgca ctctacctcg gtgagggggg cagaaggatt ctttttgctc aggatatgag
    11581 cgcacccctt cttgaggatt tcaaatgcga tccaatagca tggagggagt ctatggacaa
    11641 actccttgcc ctcggggctg acattctgtg tgacagccat gccggcgttt tcaggccaaa
    11701 acaccgggtc aaagcgtaca ttgagagatc aataatagag catggatttc agtcataatg
    11761 ctgataagct catagctgta agccaagagc aagatcatag aggggcaata ggcgaccgtg
    11821 ctttgctggg acgactgctc ttcacttgat aaccactgga acccgaaaaa agtcagtgtt
    11881 tattcgccct tcgtcaatcg gtattttttc cgctcctccc atttcttccc tatcgcctgt
    11941 aacctattac ctgtaatctg cattcctatt catttatata ggaatgtaac acctcccctc
    12001 ctgtgatggt cattcccata ctgaagcaga aggcgccggc tactatgatc gggccgggat
    12061 cggccacctc ctcaaagacc ggcggagaga attccagcca cctttcctgc tgtaatctca
    12121 tggaccgatg gcctgcccgg atcatcaatg ctcagaaagt gccgcatcaa attcaatttt
    12181 accgcaggga tatcttcaga tcttgttttg caggtaaatg tttatggaac ttaagaattc
    12241 ctctcatctg cacacctaca catttacctc agacttcata taaaaaatcc tgtttcattc
    12301 acattcaagg gttctttagc tccttactac catggagagt atccacacgt aaagtgcaga
    12361 tgcagctagc tggcagactg taatcggtaa tccataccgc aagaacgcag caaaggtaat
    12421 gggtctgccg tgagcagcac taatgcctgc actcaccaca ttagcagatg cgccgatcag
    12481 tgtagcattt ccaccgaggg tgcccccgaa catcatggcc acaaaaacag gaagggtaac
    12541 aggtggccag tctgtaaaaa atacacccag tgcatcctca gggacaaact ccgttgaaat
    12601 aagatatcct ttaacaagga gaatcatggt ggcaacgatg ggtatgtttg cgagcaagct
    12661 cgaagcaaat ccaactccta ctatcagcgc aaaggcgagg ggaaccaggt tcgatccgaa
    12721 ccactggctc atgtgactgc tcagcccgcg aagggcgcct gtcttggtga aggcctcgac
    12781 aaggatgaac atgcatgcga gaaagataat tgtcttccag tcaatatcgc gcaatacatt
    12841 tgccaccggt tccacctttg caccataaat aatcaggagg gctacagaac acgctataat
    12901 cgatgctgac gccggaacaa tacttgtcgg aagaaattct ccaaagagaa agaggagtac
    12961 catgatcccg agaaccccga gtgacaggac actgagccac ggtcgcttga atgtctcccc
    13021 cttcaaatcg tctggaagtg ttagctctat tctccatata tctttcatta tgaggggcag
    13081 aagcggtatg agcacgagga gcgacacaag cccgcctgcg ctgactcgct taaaatattc
    13141 tataaaggtt atgttgatag aggtgccgac aaggaatgtg gccgggtcgc ccacaagggt
    13201 gagcagtcct gcggaattgc tgataatcgc agtaagaacg agaggagcta ttatgtctat
    13261 cttcaaaaca gcggcaacac gaatgataat gggcgccagc agaagtaccg tggtagcgtt
    13321 cggaaggaac atgcagggta ttgccacgag tatgacaagt cccagaagaa aacgcttgcc
    13381 cctgcccctg gtctttcgaa ggaaaagggc tcccaggaat tcgaagatac ctgatgtggt
    13441 caatgtccgt gctacgatca ttccgccgaa gagaagggca aaggttgatc cggacatctt
    13501 tactacattg atgatgtcac cctgggtaag gataccgaac acaatcaaca cactcacccc
    13561 gagcaaacct gccagggcaa ggtcaaggat attgaatgct atggccagga tgactgcggc
    13621 aaagacagca taggtcgtaa cgatttgcag ttcactcatg gtatggcctc tggtaattct
    13681 cgcatcagca cgccttcaat ctcgatgaat ttatctgaat ggaggggcat acatcagtcc
    13741 ccctttattc cagagacggt ttggaccgcg gtcaagattg agagggcttc cccgtccaag
    13801 gtttcgctca aacatttctc cgtagtttcc aacactttca actatcctta cagtccagtc
    13861 attggcaatg cccagggatt ttccaaagtt tcctgatgat ccgagaaagc gctcaaggtc
    13921 cgggttacct ttctcctttg ccttggctcg cacgtttgcc tgcgtcaccc ctatttcctc
    13981 agcctctatg agggcaaaaa gcacccaccg taccagggta aaccactcgt catctccgcg
    14041 acgtaccacc ggccccaacg gttctttgga tatctgaccg ggcagaatcg cgtatgtttt
    14101 ggacccgtca cgtacacctg aacgtaccgc tgccaggtgt gaacgatcag aagtgtatga
    14161 cttgcactgt cctgaaaaaa aagcttcctg ggccttaata atggattcaa atatgactgg
    14221 cttatacctc catcctttcg ttcggaagta atcatcaagg tttgcctggc ttgtcgttcc
    14281 tttttcgatg catatggcag cattcttcag gtgagaaact tttgttattc cactactttt
    14341 gcgaaccagg aagccctgac cgtcatagta gagcgttccg acaaattgaa ggcccagcat
    14401 agtctcccgg ccaattgtcc atgtggtgtt ccttgaaaga aggtcgattt ctccggtacg
    14461 aagggctgga aggcgttccg aggctgtcaa cgggtagaaa gttacttttt caggatcacc
    14521 aaaaacagca gcagccacag cgcggcaaaa gtcagcatcc atcccggtcc attgcccctt
    14581 ggcatccttc atggaaaaac caggtaagcc ctcgctgaca ccacatcgaa gaacgccctt
    14641 tgagcgcacc ttctttaacg tctcatatga accagcaatc gacgggaaca aaaggatgac
    14701 aatgaacagt atgatgaatc tttgtaatcg ggccatccca gcctcatccc tctgttgttc
    14761 aaagtgactg aaaattttaa taaacaatgc aaaaccggta ttaaaacctt atctcctctc
    14821 aaaaaccacc acgcttctcg gtgacacccg acagtatcca tagggttgcc ctacttgctt
    14881 tcccggctcg gcaatgtcgt ctggtgatgg ctttgccgtg tcgatagcac agtaccacaa
    14941 tctcccggca gggtcaggaa gcggaatgct taagctctcc tctgacatgt tcaggacaat
    15001 atgaaggtct tcttcatcaa ccctgactgc tccgagggtg aaggccagaa cctgcgcgtc
    15061 gggatcttcc caggcaggct cataaagctt aaccccatgc caggtaacat caggataggt
    15121 tgaccctggt tcacgggtcc ctttcagaaa cctctggtgc ataagacagg gatggcgctt
    15181 gcggaaagca atcatctccc taacaaaacg gagcatatcg cggtttctgc tcgtaagact
    15241 ccagtcaaac caggatatct cgttgtcctg acaataggcg ttgttgttac ctttctgcgt
    15301 cctcagcacc tcatccccgt aaaggagcat ggggattccc tgactcaaaa gcagaatggc
    15361 catgaagttc ctggtctgcc tttgccgcag agaggtgatc ctttcatcat tggtctctcc
    15421 ttctacccca cagttccagc taaggttgtc attcgatcca tcctgattgt catcaccgtt
    15481 ggagtgattg tgtttctcgt tatagcttac gagatcgtgg acagtgaagc catcgtgaca
    15541 tgtaatgaaa ttgatactgt tactgggcaa tctcttttca cgcccataga ggtcactgct
    15601 ccccatcagt cgcgtggcaa cctccccaat aatccctttt tcgcccctga caaaacgacg
    15661 gattatatcc cggtacatgc cattccattc agcccagcgg taccctggat atcctcctac
    15721 ctggtaaagc cctgcagcat cccacgcttc tgcaatgatt ttcgtgtttg ccagggtctg
    15781 agaaagttca atgagccacg gagtaggggc aaagcgtttc gggattccgt ccatgtcgcg
    15841 ggcaaagaca cttgccaggt cgaaacgaaa cccatcaatg tgcatctccc ttacccaata
    15901 ctccagacaa tcgataatga agcgggagat tacagggtgg ttgcagttga ctgtgttgcc
    15961 acagcctgta aagtcccggt acagcctccg gtcatggggg taaagatgat aaaaacctga
    16021 attcccgaaa cctttgaaat tgatggtggg cccttctccg cccccttctg ctgtatgatt
    16081 gaacgccaca tccatgatga ctcctattcc tgcccggtga agggctttca ccatgtctcg
    16141 gaattcccgt atctgggttc ccaggtgggg ggttaccgag taaccgggat gaggggcaaa
    16201 aaaactgtgg gtgcagtatc cccaatagtt tttgagtccc aatgcagccg ttccctctgg
    16261 aacacactgt tcatcgaatg ccatggtagg catgagttca acatctgtaa tgcctaaatc
    16321 tttcaggtat ggaatctttt caatgacacc ggcaaaggtt ccgggattga tcacccctga
    16381 gttcgggtga cgggtgaacc ccctgacgtg catctcgtag ataatagtct cttccacagg
    16441 tcgctcgagt ggtttgtcgc cctcccagtc atacatgtcc tcctcgacca ggatagcccg
    16501 cattgaggca tcacagttgt ccccgggctg aatagcccgt tcacgattcc ataaaaggtc
    16561 tgtcacagca cgcgctacag ggtcgagcag tatcttttct ctgtcaaaac gatgaccggt
    16621 tcggggtgta ttgcctggcc cgtcagtacg ccagccgtaa caaatatccg gggtcaggcc
    16681 cgcaacatac acgtgccagc agaagaaggt tctgtttatc acatcgtcaa gggtgattat
    16741 ctgaaacggg tgtgggctgt cagcacactc aaaaagaaga agctcaactc ttgtggcagc
    16801 ccagcaccag attgcaaagt ttacaccttc accatcaaac gtggcaccaa gaggatagcg
    16861 atttccagga ccaatttcat aggtgcgggc attcctttcg gtggagaacg acatagctct
    16921 ttatggtggt gatacagggt cgaccatatt tcgtggctgg cccgttgcgg taagaacact
    16981 ggcccaaatc cagccatcag gatcaatctt ttttcgcctg gaaacagcca ggctgattgg
    17041 tacatgcgtg tacctgttca tccatgtacc cacgagcatg tttgttctgc ctgtcatagc
    17101 agcatgaacc gcattctggc cgaggataag ggcaaaagca gaatcagatg cgtttgccgg
    17161 aacactcctg atagtataac tgggatctat atatttcatg ctgatctgca tcgtggactt
    17221 tttgaagaaa ttgcgaatca tgtctctcag gtaatggcct atatcagcga aacgtatatt
    17281 tccggaagca tcacgctcac ctgtatcttt gaggaaattc tgacctgcgc cctctgcaac
    17341 cactatgaca gcatggcctc tctcctggag ccttttcttc agggcatgca aaaacccctc
    17401 catagtgaaa aggctttccg gtataaggca gaaattgacg tcattgcacg ccagagtagc
    17461 atatgaagcg atatgacccg attccctgcc cataagcttt acaagaccga ttccgtttcg
    17521 cgcgccgaga gcttccgtgt gggcggagaa aattgccttt tttgcctctg ccactgctgt
    17581 ctcaaatccg aaagatttct gcacatagga tatatcgtta tctatagttt tgggtatgcc
    17641 gatcacgctt atctttaatc cccgattctt gatctcttcg gaaatagcct ttgccccttt
    17701 gagggttccg tcgcctccga taacgaaaag aactccgatg ttcatacggt caagggtgtc
    17761 taccatctct gatatgtctt gaggcccccg tgatgtaccg aggatcgtcc cgcccttctc
    17821 gtgaatccct tcgactgatt tcggggttaa atcaaggggg gtatgcccgt ggcggtatga
    17881 aagcccctcg tagccgtaag gaaatccaaa cacagtcctg actccatagt gatagtacag
    17941 gcttaggacg atagatctga tcacatcgtt tagcccggga cacagcccac cgcacgtcac
    18001 aattccgcat ttcaatttcg aggggtcaaa ataaatcttc tctcttggcc cggcttgttc
    18061 aaaagcaggg ggtcttctcc cggaggccag gtacgtctcg atatcaggca gggtaaccct
    18121 aaaaagtacg ttttcattat ctttggtaaa acggccgata gagaccggcg acggtatccg
    18181 acattctccc aatttttcaa tgttcagatc gagctcatct tccatcttta tattcctctg
    18241 ttgttaaatg cttcagattg tgcaactact gaccttgtca tgcacaattg aaaataatca
    18301 atgggtttac tttaatgtag gcaggataaa aagtcaacat tatccggtaa ttccaagtga
    18361 atcttgccat gatgtgttgt aaactgtatc agggctattt catgaaaatt ctcacaaccc
    18421 ccaggatgga cagatgtatt ggctgccatt cctgttctct tgcctgtgcc cgcctcgtcc
    18481 acaggcgcct ttcatgggat actgccggaa tcagaatcgc ctcctcagga ggattatcta
    18541 caggttttga agcccggact tgtcttgcct gtgaccctgc cccctgtgtc aaggcctgtc
    18601 ccaccggggc ctacacacaa agaaaaggtg ggggagttgt ggtaaaaata agtctttgta
    18661 atcgttgcgg agcgtgcgca cgggcctgcc ctgttgatgc gatcttcctg gacaatacag
    18721 gccagccatt tgtctgtatc cactgtggcc gctgtatttc tttttgtccc catgcgtgcc
    18781 ttgaaatggg agatgtggct gctcagccca tggaaaaggg agaagaagag gtaaacctgt
    18841 gatcagtaac caattcaaaa tcctgaagat agacctttcc tcgggcaagg gaacctatga
    18901 atttattgag ggcagggacg cattcgcagg aggcagtgga cttgcagcat tgctgttcaa
    18961 cctttacggc catacagata ggccctggga tgatccggat cagccgctca tttttaccat
    19021 aggccctctg actggatact ttccgctcat gagcaagaca gtgtgcgcct tcaaatcacc
    19081 ctaccacaac cagtatgctg agagccatgg tggaggtcgc tctgccctgg cactgcggtt
    19141 tgcagatgtt gatggacttg taataaccgg gcaggccaat cgtctcagct gcctttccat
    19201 aagctccagc cacttccagg tgaaggaagc tcattacatg aaaggaatgg atgtcttcag
    19261 gacaggaaga atgctacgcc acatgtttgg aaattccggc catcgcagta ttcttcgcat
    19321 tggtcctgca ggggaacgtg gagcaccgac tgcgtgtatc aatgtggata cctatcgcca
    19381 ctttggcaga ctggggggtg gtggcgttat gggatcaaag aaccttaagg ctatcgtaat
    19441 tattggtgac aaagtctttg atttgcccac aggcgatagt tatggaaagc tctatcagaa
    19501 tgtttaccgc aaaatgactg caactgaaat tatgcacaag tatcacgaca taggtacccc
    19561 tgtaaacgtg gcatctctca atgaagttat gtccttgccc attagaaatc ttaaacagac
    19621 cacggatcca gagatggcgg gtatcacagg tgagagattt gcttctgacg ccttactccg
    19681 caaccatgcc tgttctggct gtcccgttgg atgcatccat gtagggtacg tacgggaaag
    19741 atttgccaag gaaaaccggt atttctacct ccaggtttcc tatgatcatg aaccggtatt
    19801 tgcagttggc tccatgctgg gcattacaaa ctgcttttct gtccttggca ttatcgatgc
    19861 tgtagaaaaa gttgggctcg acgtaatgtc cgccggagtc tgccttgcct gggcaaccga
    19921 ggctactgaa aaaggcctga tctctgaaaa agagacactt gtgccgcttg gatttggtga
    19981 tgcctccact taccgtcagg cagttcatca tcttggtgtt ggtacaaacg atttctaccg
    20041 ggttctttct caggggataa atgaagcagt taaacacttc ggtggttctg acttttcctg
    20101 tgtcctcggc caggagatgg cgggctatgc cacaggtgag gtttttttcg tgtcccaggc
    20161 gctgggcttt cgtcactctc atcttgatag tgccgggtat agctatgatc aaaaaaccac
    20221 agaaaaggat gtggacaggg ctgtagaatt cctggtgcag gatgaaaaag ggagagtact
    20281 cctcacgtcc atggtttcat gcctctttgc ccgtaatgta tataccgacg aggttgtatc
    20341 ggattgcctg aagagccttg gttataatgg ccttgctgta aagctggagt cagtcgcgct
    20401 taatatccag cagctccgct ggcgcctccg cttcgcctcg gggttcaatc cagatgacgt
    20461 gattatttcg aagcgcttcg gagaagtgac aactgtgaaa ggtcccatcg atatgtttta
    20521 cctggatgcg ctaaaaaagg aatatggaaa gaggataaag gctatggcct tggcagcccc
    20581 accctgatct ttctggagaa ttctggatca ttcccaatta catgtgccct tggatttata
    20641 tagcttcgtt gcctcagttc tgcgaaaggt atgtttgaat cgagcgttcc ccgttcatat
    20701 gcatactgtg gtccatatcc actgaggagt attttccagc tataatgtat acggtcgctg
    20761 aacgttttta tatgcatcac tatattagtg gtacagtttg tggtaagggt attgtaccat
    20821 actggccttt tcttcatttc attgatctgc ctgatatacg ccataaagag cttttccgca
    20881 cgttcacgat tgatatttag acggtacagg tatacatctt cctggggatt gcgataatct
    20941 gttcttaccc gtatgaggtc ccgttcatcg ccggcaatat aaatcaactc atactgtttg
    21001 aagaaccctt tgatagtcga atacccttcc cctttctcct tgcgggtttc aatagagaag
    21061 accacaaaat cttcctcctg gaagccgaaa ctcatcatga tgtgggctat cgcatcgccc
    21121 atccagtaga cggcaataag atctacagaa tcaagggctt tcagatcaaa tgttttgtca
    21181 taataatgag aatcaaagtc agtttccgta cgataggtaa ggttccggat attatggatg
    21241 gtgatctggt tgtcctgtat tgttgcatag gggagtacag caacatcagg ctgccagtca
    21301 cggtcgttgg acggcttgat attcatccac catgccacaa tcaaacaaaa gacgatcaaa
    21361 tacccgataa ggacctgcaa ccgttttttt atgaaaagaa atgctgctgc cgtaccgaca
    21421 gcaaagccaa tggcaatatt tgttcgtagg gtctcttctg ccaggtttga atagaagatg
    21481 gccagggctg cccacaaagt catgcaggca ataattatag aaaggcaaag aataacaaaa
    21541 aaccttacag tcttgcccat agctattatt cccctgattc tctgagatgt tggagtagaa
    21601 tgcggcgcac ctcttcaatg gtttcagggt ggtcctgggt tgagtgtccc gaatttacca
    21661 caagttctga agcaactccg tcgatgtgag cacttgtata ttcgacgact ccatcgttcc
    21721 atctgtcctt cggttctttc ggattcttta ccgctatgat tgaatgggcg gttacaccct
    21781 cggctatagg cattgacgag agatttttta taaacgggct tttgggatcc atgttgtccg
    21841 tgctccgggg ggcatccttt atgaatcgca cagctttcaa ctgaggattc atggtgataa
    21901 cctccttcat ggtgtcggcg agacggaagg gcaacgagat aaacttgctt gtgagccgtc
    21961 caatccagcc accagcaaca aaactgccgc catggggtgt tgcgataaaa acaacccttt
    22021 ttactgaggg gacaggttta aaaaagacac ttcgacgaag tatctctttt gtttcggcag
    22081 atgcgttgac ttcatcgaac ggcagagcag tagtccagaa cttcaacccg ctgtcaactg
    22141 ttgtcagctt tgtgaggagt ccaccctgac tatggccgat aaccaccatc cttctcaatg
    22201 ccggatcttt tccttccggg tccatctcat ctacagtttt tcggagacca tcagcaagga
    22261 tgcctgccga ataggttatc ggattgcctg tattgtaggt aaataaccag aactggtaat
    22321 taccccagag tacggggtca ttcgaaagct cattgaacat ttctgcccac ctcgcagggc
    22381 ttgatgcagt cccgtggata aatataactg gtatacggcc tggtcggtac ggctccataa
    22441 gaaagatgcc atcctggtat ctcgccttct ttccaataag ggaaaaatct cctgagaaaa
    22501 ggcctcttag ttcaaacctg tagacctggg agccctcaag tgtataagcc atagcagaac
    22561 tcagttcgta ctctaagggt acagtacgcc cgccaatatc taccgaggtt gcctctcccg
    22621 tggtgtaaag ctccaggttt gccgttactt ttccagcttc tattccctct tccacattat
    22681 caattctcag aaatgctgtg gcagcaaccc ttatcctggg agggattcgg gagtattcag
    22741 gaactattac cccttttaac ggttctgtgg cggcaataag gggagcacca atacccggcc
    22801 acctatatcg gttgcgcaat ccccggacgt caaactcggc cgcctggaca aaatctgtaa
    22861 gcctgaaaga accccaaagg aactcttcag cgtcaacctt aacgatcaat tccccgaatg
    22921 gaagagggta attgccttca ccgatcataa cctttgccct gtcaccagcc gtaaaggcct
    22981 gggccaggct tctgttgtat aaatccagtg caatacggac ccggggatca gtgggatctg
    23041 gatcagtacg gctaatcttg ggaaaaagaa aggcataagc ataaactacc gaggcgctat
    23101 aataggattg agggccacct ttagaagcat aggcatagct cagctcagcg agggcaaaaa
    23161 accgatctgc ttcactcgcc gttgggagac ctttgtggag ggctgccaga acttctgccg
    23221 gttgtgttct aaatctggct gaaagatcag cacgattgag gatttgctgc gtgtaagcac
    23281 tcagctcatc actcgaaagg atacttgccg taaggtttct ctgaacctgt ttcggatcaa
    23341 gctgacgcac cccaacaggg gttgcgcacc caccgaggaa catagcgata aaaaccagga
    23401 ggccaatgta tgcgacgtac ttcggatact taatcctatc tctgcatctt ttcatcatat
    23461 atactcctgc ctgattttaa tgatcaccaa atttccctac cctatgatca cgtattgtac
    23521 cggtttactc tataactctc aataatattt aaagttggtt ttgtaacata gcccacattc
    23581 acttacagat cattgcctca atggacaggt cagttacttc ttttaaaatg gaggctgaac
    23641 agaaccctgg caaccaaaaa ctcatttgat agttttgagt ta
//
LOCUS       DAOOMC010000030        23375 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdus sp. isolate AMR_MDS_2685
            NODE_2087_length_23375_cov_6.461492, whole genome shotgun sequence.
ACCESSION   DAOOMC010000030 DAOOMC010000000
VERSION     DAOOMC010000030.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864760
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdus sp. (wastewater metagenome)
  ORGANISM  Syntrophorhabdus sp.
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae; Syntrophorhabdus.
REFERENCE   1  (bases 1 to 23375)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23375)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,729
            Genes (coding)                    :: 2,708
            CDSs (with protein)               :: 2,708
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 7 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23375
                     /organism="Syntrophorhabdus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2087_length_23375_cov_6.461492"
                     /isolate="AMR_MDS_2685"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2699756"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            205..1014
                     /locus_tag="PK125_04585"
     CDS             205..1014
                     /locus_tag="PK125_04585"
                     /inference="COORDINATES: protein motif:HMM:NF013757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HPB37419.1"
                     /translation="MYNAPILKKAIEILRMLIREHQPLGVTEIARAVSISKSTAYGIL
                     KSFSEEGLVSKNPTTKKYSLGKEMMELARLVFRGQDLISIARPFLERLVELVNETVFL
                     GIREFDAIKVIDVIEAKKEIKISSPVGMKLPITAGATGKLFISIMTNEEIVSYFREKG
                     LPRYTENSITDMDAFIKNMEEIRRLGYSIDIEEYLKGIRAVATFIRQDKKPIAAIWFV
                     GFSNSMIDERLNHIINNLQRTADQISARASLSSSSELEAPVVLKNEISSLV"
     gene            1211..2947
                     /locus_tag="PK125_04590"
     CDS             1211..2947
                     /locus_tag="PK125_04590"
                     /inference="COORDINATES: protein motif:HMM:NF013481.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016625 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, iron-sulfur
                     protein as acceptor [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase C-terminal
                     domain-containing protein"
                     /protein_id="HPB37420.1"
                     /translation="MDKILRIDVGADGGPKATTDPVGDYAGLGGRALTSAIVSKEVPM
                     ACHPLGEENKLVIAPGLLSGSIAAMSGRISVGAKSPLTGGIKEANSGGQPSQVLARLG
                     YAAIVLQGKPKDDSLYKVFINKNGVEITKDNSLKMLGNYDVVEKMKEEYGQKIACISI
                     GQAGEMKLAASSVAFTDMELRPSRHAGRGGLGAVMGSKGIKVIVLDDAGMAMRQPKDV
                     ERFKEANKRWIAGLQGHGVTGQALPAYGTNVLTNIINEAGAYPTYNFKEGRFAGAAKI
                     SGEVLAEMETKRGGLATHGCHSGCVIRCSGIYNDKDGHFLSKQPEYETVWAHGGDCGI
                     DDLDTIAMLDFLDDNYGLDTIEMGVTIGVAMEAGLLKFGDGKGAINLIKEVGQGTPLG
                     RIIGSGAVVTGKVFGIERVPVVKGQAMPAYDPRAVHGIGVTYATSPMGADHTAGYAIA
                     SNILGVGEKVDPLKPEGQVDLSRALQIATAFIDSSGMCVFVAFALLDQPETNQALIDL
                     VNAFYGWNWTADDTVEFGKRVLKMERDFNVGAGFTKEHDRLPLFMKTQQLSPHNLTFQ
                     VKDEELDGVFNW"
     gene            3016..3651
                     /locus_tag="PK125_04595"
     CDS             3016..3651
                     /locus_tag="PK125_04595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB37421.1"
                     /translation="MANQNPQHDIDASVLNINLTVQLSAVKAFFLLLQNGFSIKCLTG
                     ISIEQLLYNQLGLTPQLVEKKISTVFLDGKPVDDIAMAIVQDGSSLALSGAMPGLVGA
                     TLRRKSPLASFRQSISVESGLGKSEPIQGHVMVKLFNVLLKELGPFFLRTGIFVSAPA
                     FVDLVSGKADDFLDKCIQVVVNGVSVTLQSQGEWHSLLASHDSVHLKVKVG"
     gene            complement(3938..5428)
                     /locus_tag="PK125_04600"
     CDS             complement(3938..5428)
                     /locus_tag="PK125_04600"
                     /inference="COORDINATES: protein motif:HMM:NF017669.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF853 family protein"
                     /protein_id="HPB37422.1"
                     /translation="MNQILIGKGERPVHLLAKYGNRHGLVAGATGTGKTISLMVMAEG
                     FSRMGVPVFMADVKGDVAGLAMAGTANEKIQQRVKEIGIDDYTNEANPVLFWDMYGKN
                     GHPVRTTISEIGPGLLGRILEINDTQTGTLEIAFKFADDQGLLLLDLDDLRALLSFVA
                     DNRKEVSAQYGLVNTQSIGAIQRALLTLEREGGQALFGEPALELNDLMRTDLSGRGII
                     SILNADQLILKPRLYSSFLLWLLSELFENLPEVGDLDKPKLVFFFDEAHLLFDDAPPV
                     LRQRVEQVVRIIRSKGVGVYFCSQFPDDVPNEILGQLGNRIQHALRAYTPRDQKAVRT
                     AAETFVTNPKLNVTEVISQLAVGEALVSTLQDKGVPMPVERTTICPPRCRMGTLTSEE
                     RSVVRARSPIGAKYDISVNRESAYEIINQRARNKEAVQPETKGKEETKPVESGGKLGD
                     LLWGSKRREGMVQAMAKQAARTVGSQIGRQIIRGVLGGILGGSGRK"
     gene            5721..5945
                     /locus_tag="PK125_04605"
     CDS             5721..5945
                     /locus_tag="PK125_04605"
                     /inference="COORDINATES: protein motif:HMM:NF014636.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HPB37423.1"
                     /translation="MQVTIKLFATLRQGRFDVDTLNLPPKTKVTDVIQQIGVPHKEVT
                     LIFINGRHGDFSSELHEGDILALFPPVGGG"
     gene            6082..6918
                     /locus_tag="PK125_04610"
     CDS             6082..6918
                     /locus_tag="PK125_04610"
                     /inference="COORDINATES: protein motif:HMM:NF013094.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HesA/MoeB/ThiF family protein"
                     /protein_id="HPB37424.1"
                     /translation="MKKLDEFLKANAKDELLSWDAHKKAMKVFRLGCRHVEEAALALN
                     ILPARYKRNQKSITTALQRKLFQSKSAVIGCGGLGGYIIEELARLGIGTIKAIDPDIF
                     EEHNLNRQILCKVSDLGKPKAKVAKTRVKTVNPAVKIISLVKAFSRENGPELLEGMDV
                     VVDALDSIPTRIELAECCAKLNIPLVHGTIGGWYGQITTQFPGDNTIQIIYQNCKDSK
                     GLEIKLGNPSFTPAIIASLQVAEVCKLLTGQGTPLRKKMLFINLLDMEVDQIEIGQTI
                     SC"
     gene            7054..9288
                     /locus_tag="PK125_04615"
     CDS             7054..9288
                     /locus_tag="PK125_04615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB37425.1"
                     /translation="MAGLVVGSLAAFFKQRSSKPLNPSSSQALQRLAAALVASCPVVF
                     ITLSVIPLDHVRMLWEKVQIVYFVLFILLCTIPFLLYGAFIAFSLSVWRDKANKVYAS
                     DLIGGATGLLLVAMLMNLLRTEYVFAVTIAVTGLAILAVLENTLSKIIAGSIFFILCL
                     LVVLDVSAIKMSPHKGLVQALKVDGATHVTTIYTSHSRLDLFENPQMKFAPGLSLGYT
                     QPVPKGLGVALDGEIAGVIMGGEQQESYDFLSYVPSALPYLLLQPKNVVIVGAKNGID
                     LLQPLFFGAFHISVTEYDASVLRAFASIHDEYSPRTVYFFHGSARRLLRSLPQEPDII
                     LLSRTGFFPSGNFGLQEDYDLTVEAITSYIKGLKKNGILFVQMFLQPPPRNELRLVMN
                     IRAALNKMGVAGLSKQLLIYRSWDTVNILVKKDGFSETDLNKIGQFLSNRQFDILYPG
                     MAGQEKFITGLDYEALFNKILVHESSADFSSSYVFDIRETTDDRPFFHYYLKLSNIRE
                     IYRISGRKWPYLIHEGMFLPFVLIFLVVIAIGIFGSTLLFLKRRGAGNLQLNTGLLIY
                     FALIGFAFMFTEMFFIHRLILPLGSPVRAFSITLVTILMSSGIGSMLTVWFIERKMIW
                     IIGLTPLIVIANYIIFDIIDETALSVFSVVPIGILLGLFFPVGLRFLVSKETDGGVPL
                     AYAANGTASIIAPSLASLVAVSCGCSILLILAAIFYALAIVILLFATRRISWMSKHTW
                     TFLF"
     gene            complement(9337..10080)
                     /locus_tag="PK125_04620"
     CDS             complement(9337..10080)
                     /locus_tag="PK125_04620"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: protein motif:HMM:TIGR00080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HPB37426.1"
                     /translation="MRETAYTAHIKKWSLTALLFLASTIVCQSVAFPDDIFDKRRQEM
                     VEKDIKGRGVKDALVINAMLTVPRHLFVDESRQNKAYADHPLPIGDGQTISQPYVVAL
                     MTEVVGLKGNERVLEIGTGSGYQAAILSAIVKEVYTIEIKKSLYETAKTRFQRLGYRN
                     IRTKHGDGYYGWESYAPFDVIMVTASANHIPPPLLAQLKEGGRLIIPLGSTVFYQNLA
                     LVTKEKNRYSIKELGGVTFVPMTGQAQKK"
     gene            10252..>11351
                     /locus_tag="PK125_04625"
     CDS             10252..>11351
                     /locus_tag="PK125_04625"
                     /inference="COORDINATES: protein motif:HMM:NF013793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HPB37427.1"
                     /translation="MLINQLDETAMRDVRVACATLFSEELAYDDSFIENLSIETVEKA
                     YWQKAKRCYHHLSPLSSRSEAQEALNDVRNSLETLTSFLRTDSQMVDEAPRIGKIIAI
                     GGAKGGIGKSIIVANLSVLLASKGFRVTAVDLDLGGANLHLCLGNKVLLQNNINDFLR
                     KRVDSLQDIAIKSEYGPYLIGGDSAQLGAANIDFGRKLKLLRAVENLNSDFIILDLGG
                     DTSYNIIDFFLKADYGIVVTTRDSASYIGAYHFLKAAMYRRFNRLFGPESRFRMYKNH
                     QLEQLIREMMNPADGGWPKSINDLLEKVRQEQPDHFPFMCEVVENFNSYLVVNKVPNR
                     LLRTFDVNPIITRIQQVTRTWLAKEVTYLGSIS"
     assembly_gap    11352..11451
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<11452..12533)
                     /locus_tag="PK125_04630"
     CDS             complement(<11452..12533)
                     /locus_tag="PK125_04630"
                     /inference="COORDINATES: protein motif:HMM:NF024091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPB37428.1"
                     /translation="MFERIKNLVIKEFIYYFREKRTVFFLLATPLIQIIVFGYVATMD
                     VNNVSTAIYDLDRSSESRELVRRLESSGYFTVKRVPASPEEVRELIDKGDVLCAIQIN
                     HGFGRDIQTGIGTDIQVIVDGTDSNTALIAMSYVNTIIGKYSRDMMDARIKANVWSLD
                     LRSRVWYNPDLKSRNYMVPGVIALIVMLTCLLLTSMSVVREREVGTMEQLMVTPIRPL
                     ELILGKTIPAAIIGYFDMALVTVFGVFWFSVPINGAVLLLVVCTGAYLLSVLGFGLLI
                     STISKTQQQAMTATFFFFQPVILLSGFATPIETMPEVFQYMTYLSPLRYFLIIVRGIF
                     LKGVGVDILWPQIVALLVLGTVILAA"
     gene            complement(12536..13645)
                     /locus_tag="PK125_04635"
     CDS             complement(12536..13645)
                     /locus_tag="PK125_04635"
                     /inference="COORDINATES: protein motif:HMM:NF024091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPB37429.1"
                     /translation="MKLLRIKAITKKEFIQIWRDPLSLAMAFIMPVILLFIYGYAITF
                     DIDKIPTVVFDMDKSSLSREFVNQFHESGYFRVNSYVESYKAIDPYLDSGRARVAIVI
                     PEYFSKNIKKGFPVQVEVLIDGGDSNTATIAQGYSTAIAEGFSQRVRGSRMSPLIESR
                     SRVWYNNELKSRNFIIPGLIAIIMAVIIALLIALTVAREWDRGTMEQLISTPIKTPEL
                     ILGKLIPYFLIGFAGTLLTILMSTLLFDVPLRGSIALLLGLSAIFLFGGLSFGMLMSI
                     AGKTQLVASQAALLTSFLPAFLLSGFIFSISNMPKPLQVITYFIPARYFVTILKGIFL
                     KGSTLRVLWLEALLLTLFAVLVFIAANRKFRKRID"
     gene            complement(13702..14643)
                     /locus_tag="PK125_04640"
     CDS             complement(13702..14643)
                     /locus_tag="PK125_04640"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPB37430.1"
                     /translation="MIDNDMNGNSIAVQVNDLRRTFGDFVAVDNISLSVRRGEIFGFL
                     GPNGAGKSTTIRMLCGLLMPTGGTGTVGGFDINKESELIKKVIGYMSQKFSLYDNLTV
                     EENINFFGGVYNVPKEKKIERKEWVLNMAGLKEERHALTRTLPAGFKQRLALGCAILH
                     DPHIIFLDEPTSGVDPISRRNFWNLINGMSQAGTTVFVTTHYMDEADYCDRLALIYRG
                     KIVAEGTPTDLRRKHMTRDVLEVNVDKVVEAFNTLTFHKIEAAIFGSTLHVVVDDEET
                     FRERISSVLAESGVAVKGIEKIPPSLEDVFVTLIEVS"
     gene            complement(14716..15654)
                     /locus_tag="PK125_04645"
     CDS             complement(14716..15654)
                     /locus_tag="PK125_04645"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPB37431.1"
                     /translation="MNSEIAIRTENLTKAFGGNVAVNNLNLEVKKGELFGLVGPDGAG
                     KTTIMRLLAAIMEPTSGNAWVAGYSILTEGELIKEEIGYMSQRFGLYEDLTVMENIMF
                     YADLYGVPARERPPRVERLLGFSNLTPFKERLAGNLSGGMKQKLGLACALIHTPRVLF
                     LDEPTNGVDPVSRRDFWKILYDLLKEEITVFVSTAYLDEAERCTRIGLIHKGTILTVD
                     EPSSIRESLQIPMVEISSAKAKVISDVISTIDGVINVSMYGDRLHVGIKEKGIVDQVL
                     TVIRGQGVEIEGYREIVPSLEDAFIALVDREEGKRP"
     gene            complement(15714..16070)
                     /locus_tag="PK125_04650"
     CDS             complement(15714..16070)
                     /locus_tag="PK125_04650"
                     /inference="COORDINATES: protein motif:HMM:NF008911.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HPB37432.1"
                     /translation="MVDKIKTHKDLDVWKESIELARLVYLMTSKFPKEELYGITQQIR
                     RSAVSISSNIAEGAARNSKKEFIQFLYISLGSLAELETQLLLSEKLGFLQNIGCETNI
                     ERIRKMLVGLIKYLKS"
     gene            complement(16124..17110)
                     /locus_tag="PK125_04655"
     CDS             complement(16124..17110)
                     /locus_tag="PK125_04655"
                     /inference="COORDINATES: protein motif:HMM:NF027890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HPB37433.1"
                     /translation="MKKKRLLIAGIIILIIITIFLINHFRSKNDNNALNLSGNVEVTE
                     TNIGFKLPGRVIELAVDEGQQVRSGQLIARLDNAELASVVMQHKATLQEAMARLAELK
                     SGSRIQEIERARASVDAQAADLDKAKKDYERADILYKNGAISTSQFEAAQNAYNTRTA
                     QYRSAQETLSLVQEGPRKEDIQAAEYRVQQAKAMVNTSEERLKDTTLFAPITGVVLRK
                     NVELGETLSAGTPVFTIGDLENPWVKVYVKEDRLGQVKLGQKADIKVDSFKDKTYDGT
                     VTFISSEAEFTPKNVQTEEERVKLVFGVKVKVKNENGELKPGMPADVRITLK"
     gene            complement(17182..18621)
                     /locus_tag="PK125_04660"
     CDS             complement(17182..18621)
                     /locus_tag="PK125_04660"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HPB37434.1"
                     /translation="MSGKFSARSAGGSLKIMGFIPCLILALIFIVHVGPSQAQQNSLS
                     KSGETPSIIIKTDDNLLKPQGERYTLSEAIAYALKNNPRVRISAKDIDTEAYGIDAAR
                     AERMPRIDFGSSASRYRYPAPLTPPVISGLFGAGPDLPEYSRNIYDAGGSLRLPIFKG
                     GRLYRAVLVAELRKGIAQENLVISKQDLIFNMSSVFYKIAELEKLYVSTDGTVKQLEA
                     HKSDVEVFYKTGAVPYIDLLKTDVQLAHAVEQRLAVKNNRESAYELMKNLMGMDDTEK
                     NIVIDYQKAPPQLLPSLEESLTKSLVQRPEYRAVRKKRMISEERVKMAQGKRLPDVYV
                     GGEYGGKAGDSFGFKENWYFGARMSIPVFDGGLISAEINRERAELQKVLEEERLIRLA
                     ITREVRDAYLNITHARERTEVAGKAIESARENARIEKLKYQTGAGTNTDYLDAQTDLL
                     RAEADYYRSFFDEEVARASLRKAMGETGY"
     gene            complement(18618..19283)
                     /locus_tag="PK125_04665"
     CDS             complement(18618..19283)
                     /locus_tag="PK125_04665"
                     /inference="COORDINATES: protein motif:HMM:NF020771.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CerR family C-terminal domain-containing
                     protein"
                     /protein_id="HPB37435.1"
                     /translation="MKKNHQNVESTPGTRERILQAAAEVFADSGFERATVREICSRAS
                     VNVAAINYHFRDKENLYIEVLKYSKALAFKKYPHDLGTQKGDSPEIRLRVFIRSLVLR
                     ILGDDLSSLFGRLVSREYIEPTSAFDVIVEETMRPTFAFLSEIIGELLGGKVPAMRVH
                     MCCASVISQCLFFLYARHALAKLFPEQKFGRTEMEAIADHIAGFSLSAIKDLRKTMKG
                     ERK"
     gene            19475..20026
                     /locus_tag="PK125_04670"
     CDS             19475..20026
                     /locus_tag="PK125_04670"
                     /inference="COORDINATES: protein motif:HMM:NF021561.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2284 domain-containing protein"
                     /protein_id="HPB37436.1"
                     /translation="MRKTKVPDVVKTKLRPYLKTALDNKITDAIVIKTSAVFTAPWVR
                     IKCQFGCPGFGETLCCPPHTPTPDEMRKILDSYTYSILLHLHWTKNYRIANKFNDILV
                     YLERMIFLDGYYKAWALGSGPCDRCKTCNIDGTCRNADKARPSMEGCGIDVFKTAREQ
                     GLPINVVQNHGAERDLYGLVLVE"
     gene            complement(20465..21292)
                     /locus_tag="PK125_04675"
     CDS             complement(20465..21292)
                     /locus_tag="PK125_04675"
                     /inference="COORDINATES: protein motif:HMM:NF012933.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB37437.1"
                     /translation="MKGKILTTEEIRAFQRQIYEYYKHHGRDFPWRRTTDPYNILVSE
                     IMLQQTQVERVHDKYLEFIKMFSNFNVLAEASQGDVLAMWQGLGYNRRALALKKLALE
                     IVEVYGGVLPQDVNKLSSLPGIGKTTAGAILAFAFNMPSVFIETNIRRVFIHEFFRKK
                     EDVKDGEIFPLVEATMDRENPREWYWALMDYGSMLKRKTENPNRRSAHYVRQPSFAGS
                     DRQIRGLIIKKLLDESNLSVKDLNESIGINLDHLGRILRGLIHEGFICEENGFYKIV"
     gene            21378..22943
                     /locus_tag="PK125_04680"
     CDS             21378..22943
                     /locus_tag="PK125_04680"
                     /inference="COORDINATES: protein motif:HMM:NF025052.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HPB37438.1"
                     /translation="MHASETPRKLVSFLLDPQSYPENTANVTHIETHISHVFLCDSFV
                     YKIKKPVNFGFLDFSSLKKRYYFCRKEVELNARLAKGIYLGVQPIYNADGCYSFVNNK
                     EGSPVEFAVQMKRIPMECLLFNLIEKGRPLYRDLEEVGRTLAFFHTNIKPYRGKRFGG
                     FEAILAATEDNFEQIKQFCGITVNENTFETIASYTREFLEKNKNKFYSRKQNGFIREG
                     HGDLHCQHICLEHPPVIFDCIEFNEAFRIIDVLEDIAFLFMDLEYRGRFDLTSCLSKA
                     YFADLQKVQDEDLLRFYKVYRAVVRGKVEGLTAENVTTAKEQKLSTAKAMNYFDLACY
                     YINDAHKAFNPIVFMGLSGSGKSTIAMGFSSDADILHSDEIRKAVAGLGKNEHHYIEV
                     GKGIYTPELTGQIYYTLLDKAVGCAKEGKKVIVDATYLKANQRTHFYSSCIETGLNPF
                     FVHCFASENILRKRIIERMQNGRDISDAHLSVLEHQLKHLEEPEELPSFRVLKINTED
                     AIENIVNTLKEFL"
     gene            complement(23081..23344)
                     /locus_tag="PK125_04685"
                     /pseudo
     CDS             complement(23081..23344)
                     /locus_tag="PK125_04685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016535194.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
BASE COUNT         6271 a         5388 c         4980 g         6636 t
ORIGIN      
        1 atgtctctga ggatggcaga gaaggattcc aggccttcat tgagaagaga aaacccgatt
       61 ttaagggaag ataagatttt gaatagaagg gtgcgctcta ccaattactg ttgaataata
      121 ttacatagct ctatccccaa gccctgtaaa gtcagggctt ggggatattt cacggcagac
      181 agcgcactca catcgtaaac aatcatgtat aatgcgccga ttcttaaaaa agctattgag
      241 atattacgca tgctgatcag agaacaccag cccctcggag ttacagagat tgcacgggcg
      301 gtgtcgataa gcaaaagcac tgcttacggt atcttgaaaa gcttcagcga agaaggtctt
      361 gtcagcaaga acccgacaac aaaaaaatat tcccttggca aggaaatgat ggagcttgca
      421 agactcgttt tcagaggaca agacctcatt tctattgcca gaccctttct tgagaggctg
      481 gtagagcttg ttaacgaaac ggtctttttg gggatacgtg aattcgacgc aataaaagtc
      541 attgatgtta ttgaggccaa gaaagagatt aagatatcat cacctgttgg aatgaaactt
      601 ccgatcacag cgggtgctac gggtaaacta ttcatttcta ttatgaccaa tgaagagatt
      661 gtatcctatt tcagggaaaa aggactgccc cggtacactg aaaatagtat tactgatatg
      721 gatgcattta ttaaaaatat ggaagagatt cgcaggctcg gctatagcat agatatagaa
      781 gaatacttga agggtatcag agctgttgcc accttcatcc gacaagacaa aaaacctatt
      841 gccgccatct ggtttgtcgg tttttcaaat tccatgattg atgaaagact caaccatatc
      901 atcaacaact tgcaacgaac agcagatcag attagcgcgc gagcttcctt aagctcttcc
      961 tcagagctgg aagcaccagt agtattgaaa aacgagatat cttctctggt ttaacatcct
     1021 gaattccttt ttataaatct ttcgtttatt attgattatc attttctgag aaatattttt
     1081 ccattttgaa aataaataat aatgtaataa acttaatgca gggcaggctt ttttatcagg
     1141 cagaacattt gtgatttttt tttgagttga ggctgaaagt tttgccaaaa aatagcagag
     1201 gagggacagt atggataaga ttcttaggat tgatgtaggt gctgacggtg gcccgaaggc
     1261 taccacagat cccgtaggtg actatgcagg ccttggtgga agggcgctga catcagccat
     1321 cgtgtcaaaa gaggtgccga tggcatgcca tccgctgggt gaagaaaata agcttgtcat
     1381 agcccctggg ctcttgagcg gctcaatagc tgcaatgtca ggtcgaatct cagtgggtgc
     1441 aaaaagccct ttgaccgggg gcatcaaaga ggccaattct ggcggtcagc catcacaggt
     1501 gcttgcacgg ctcggttatg ctgcaatcgt actccaggga aaaccaaagg atgatagtct
     1561 atacaaggtt tttattaaca aaaatggagt ggaaatcacg aaagacaaca gcctgaaaat
     1621 gcttggtaac tacgatgttg tggagaaaat gaaagaggaa tacggacaaa agatcgcctg
     1681 tatctcaatc ggtcaagcag gggagatgaa gctcgcagct tcttcagtag ccttcacaga
     1741 catggaactc agaccaagca ggcatgctgg aaggggtggt ctgggagcgg tcatgggatc
     1801 caagggtata aaagtcattg ttcttgatga cgcaggtatg gcaatgcgac aacccaagga
     1861 tgttgaaaga ttcaaagagg ctaataagag atggatcgca ggtcttcaag ggcatggcgt
     1921 tactggacag gcactacctg cctatggcac aaatgttctc accaatatta tcaatgaggc
     1981 tggagcttac cccacctaca attttaagga aggccgtttc gccggagcag caaagataag
     2041 cggtgaagtt ctggctgaga tggagaccaa aagagggggg ctggcaaccc atggctgtca
     2101 cagcggttgt gtcattcgct gctcaggaat ttataatgac aaggacggtc actttctctc
     2161 aaaacagcct gaatatgaga cagtgtgggc tcatggtggg gactgcggca tagatgacct
     2221 ggataccatc gccatgttgg attttctcga cgataattat ggcttagata caattgaaat
     2281 gggtgtcacc attggtgttg caatggaggc tggccttttg aagtttggag atggcaaggg
     2341 agccattaac ctgataaagg aagtcggtca gggaacaccc cttggcagga ttattggaag
     2401 cggcgcagta gtgacaggaa aggtttttgg cattgaacgt gttcctgttg tcaagggaca
     2461 ggccatgcca gcctacgacc cgagagcagt tcatggcatt ggcgtcacat acgcaacaag
     2521 ccccatgggc gctgatcata cagcaggata tgccatagcc tcaaatattc tgggggttgg
     2581 tgaaaaagta gatcctctca aacctgaagg acaggtagac ctttcaaggg cactccagat
     2641 cgcaacagcc ttcatcgatt catctggcat gtgtgtcttt gtggcttttg ctttgctgga
     2701 tcagccagaa accaatcagg cgttgatcga ccttgtcaat gccttctacg gatggaattg
     2761 gaccgcagac gacacagtcg aattcgggaa aagggttctg aagatggaac gggatttcaa
     2821 tgttggagcc ggttttacaa aagagcatga tcgactcccg ctttttatga aaacacagca
     2881 attgtcgccg cacaatttaa cttttcaggt caaggatgaa gaattagacg gtgtttttaa
     2941 ctggtagaca cttttgaagc ttaatgagga aggggcaaca tagcgcacct tcctcatctt
     3001 atttcaaaaa gatacatggc aaaccaaaac cctcaacacg atatcgacgc ttcagttctc
     3061 aatatcaatc tgactgtcca actttcagcg gtgaaagcat tctttctctt acttcaaaat
     3121 ggcttttcca taaaatgtct taccggcata agcatcgaac agttactcta taatcaactg
     3181 ggattaacac cacagctcgt tgaaaaaaag ataagcacag tctttcttga cggtaagcct
     3241 gttgatgata ttgctatggc aatcgtacag gacggttcat cattggcgct ttcaggcgcc
     3301 atgcccggcc tggtcggggc aacactgcgt cgaaagagcc ctctcgcttc attcaggcaa
     3361 tcaataagtg ttgagagtgg cttgggaaag tcagaaccaa ttcaaggaca tgtcatggtc
     3421 aaacttttca atgttctcct caaagagctt ggcccttttt tcctcagaac aggtattttt
     3481 gtttcagccc ccgcttttgt tgacctcgta tcaggaaagg ctgatgattt ccttgacaaa
     3541 tgcatacagg tagtggttaa cggggtgtcc gtcacattac aatcacaggg cgaatggcat
     3601 tcccttctgg caagtcatga ttccgttcac ttgaaagtaa aggtaggata atccagatat
     3661 tttctataag ttccctcgtc agcctgtcag gcgacgaaag gcaactgtag tcttgctgaa
     3721 accatgatca gcagtatgag gattcggatc tccgcgtcac gtggctcgat agtctccctg
     3781 atgctaccac ccttcagaat ccagtgttct gtgtaatcct attcgctaac ttaggattgt
     3841 atacccacga accaattaaa atcttgtagc acagatttcc agtgtccgct aataaaccat
     3901 ccctattaaa aatcagggtg ataattggtg acgcaaccta tttgcgacca gaccctccaa
     3961 gtatgccgcc gagtacacca cgaataattt gccgtccaat ttgactccca acggttctgg
     4021 ctgcttgttt ggccattgcc tgcaccatac cctctctccg tttggagccc cagagcaaat
     4081 cacccagttt tcctccgctt tccactggct tcgtttcctc tttccccttt gtttcgggct
     4141 gaacagcttc cttatttctc gctctttgat ttatgatttc ataagcagac tcacgattca
     4201 cagagatatc gtacttggcg cctatggggc ttctcgccct caccaccgag cgttcttcag
     4261 acgtcaaggt ccccatccgg caacggggcg ggcatatggt agtgcgctcc accggcattg
     4321 gtacaccttt atcctggaga gtggaaacta atgcctcgcc aaccgcaagt tgcgatatga
     4381 cttcagtgac attcaatttg ggattggtaa caaatgtttc tgcagcagtc cggactgctt
     4441 tctggtctct tggcgtgtaa gctctgaggg catgttgaat gcggttccca agctgcccga
     4501 ggatttcgtt cggtacatca tcaggaaact gtgaacaaaa gtaaacccca acacccttgg
     4561 agcgaataat ccgtacaacc tgctctaccc gctgacgcaa tactggtggt gcatcatcaa
     4621 agagtaaatg ggcctcatcg aagaagaata caagctttgg cttatcgagg tcacctacct
     4681 cagggaggtt ctcgaacagt tctgataaaa gccacaggag aaagctcgag tacaatcttg
     4741 gtttcagaat gagctgatct gcattaagaa tgctgataat gcctcttccg ctgaggtcag
     4801 tacgcatgag atcgttgagt tccagagcag gttcgccaaa cagtgcctgt cccccttcac
     4861 gctcaagggt cagcaaggct cgttggatgg cgccgataga ctgggtattc accagaccat
     4921 actgcgctga tacctccttc cggttgtctg ctacaaaact gaggagagct ctcaaatcgt
     4981 caaggtcgag cagtaacaag ccctgatcat ctgcaaactt gaaggcaatc tcgagcgtac
     5041 ctgtctgcgt gtcgttaatc tcaaggattc gaccgagcag gcctgggccg atttcactta
     5101 ttgttgtacg gacagggtgg ccatttttgc catacatatc ccagaataag accggatttg
     5161 cttcgttggt gtaatcatcg atccctatct ccttcacacg ttgctggatt ttttcattag
     5221 ccgtgccagc catagcaagg ccagccacat caccttttac atcagccatg aacacaggca
     5281 cgcccattcg tgaaaaaccc tctgccatga ccatgagaga aatagttttg cctgttcctg
     5341 tagctccagc tacgaggcca tgtcggttgc catactttgc gagtaggtgg acaggccgtt
     5401 ctcccttgcc aatgagtatt tggttcatcc ataacctcct gcacttttca ttgtatttat
     5461 ttgattacaa aattcaggca taacgtatct cctgtgtatg acgattatca tcatattcta
     5521 ctatcttttt acctctccct gaaatagtat attaaattca cggttgagtc actatatctc
     5581 cagcataaga ttgtcatttt gtacaattca ttgtcgttat gtaaccttct ctcacgtaga
     5641 aaatgatgca atttccacct ccaaagattt aagggcgttg tttttgaaaa acccctgtgg
     5701 tattttggat aaggcataca atgcaagtga ccattaaact ctttgctaca ctgcgacagg
     5761 ggcgttttga tgttgacacg cttaacctgc cccccaaaac aaaagtcact gatgtcattc
     5821 agcagatcgg ggttccccac aaagaggtta ccctcatctt tattaatgga agacatggtg
     5881 atttttcatc agagcttcat gaaggcgata tattagccct atttccccct gttggaggag
     5941 ggtgagacag gcttggagct tggtgcatgg cgtatagagg taataaagtg aaaaatttat
     6001 cacaccgcgg gcattacaga ttttgggctt tattatgtgc tctcaactat cagctatgag
     6061 ctatttatgg aggatacgcg gatgaagaaa ctggatgagt ttctgaaagc caatgccaag
     6121 gacgaattgc tgtcatggga cgcccataaa aaggcaatga aggttttccg ccttggatgc
     6181 cgccatgtag aggaggccgc ccttgcactc aacatactgc ctgcccgtta caagcgcaat
     6241 caaaaaagca tcaccaccgc tctgcaacga aaactttttc agagcaagtc tgctgtcatc
     6301 ggctgtgggg gattgggtgg ttacatcatc gaggaacttg cacgccttgg cataggaact
     6361 atcaaggcaa tcgatccgga tatctttgag gaacacaacc taaatcgcca gatactgtgc
     6421 aaagtttccg acctcggcaa accaaaggca aaagtggcta aaaccagagt gaaaacagta
     6481 aatcctgctg taaaaatcat ctctctggtg aaagcatttt ctcgggaaaa tgggccagaa
     6541 cttctcgaag gcatggatgt tgtggttgac gctctcgaca gtataccaac gcgaattgag
     6601 ctggcagagt gctgtgcaaa actcaatatc cccctggttc acggcactat tggtggctgg
     6661 tacggtcaga taacaacaca gtttcccggc gacaatacga tccagataat ctatcagaat
     6721 tgtaaagatt ccaaaggcct tgaaataaag ttgggcaatc cctcctttac cccggcaatc
     6781 atagcaagcc tgcaggtggc ggaggtatgc aaactcctta caggccaggg cactcctttg
     6841 cgtaaaaaga tgcttttcat caatctgctt gacatggaag ttgatcaaat tgaaatagga
     6901 caaacaatat cttgttaatc acataatctt gccatgccct atctgcttgt ctttttttgc
     6961 tccttcatca ccctctcatt tgaaatacta cttattagga tcttctctat aaggctttca
     7021 catcactacg cctctcttat cattagtctg tctatggcag gccttgtagt ggggagcctg
     7081 gctgcgttct tcaagcaacg ctcatcaaag cctcttaatc cttcgtcttc tcaagctctt
     7141 cagcgccttg ctgctgccct tgttgcctca tgccctgttg ttttcataac actgtcagta
     7201 attcctctcg atcatgttcg aatgctctgg gaaaaagttc aaattgttta tttcgtactt
     7261 tttatcctgc tttgtaccat cccattctta ctttatggcg cattcatagc attctccctc
     7321 tcagtatggc gcgacaaagc aaacaaggtt tacgccagtg atcttatcgg tggtgccaca
     7381 ggacttctcc tggtggccat gctgatgaac ctgctcagga cagaatatgt ttttgcagtt
     7441 acaattgctg tgacaggttt agccattctt gcggtactcg aaaatacatt atcaaagatt
     7501 atagcgggct ccatattttt tattctgtgc cttttagttg ttctggatgt ttctgccatt
     7561 aaaatgtctc cccacaaggg acttgtacag gccctgaagg ttgatggggc cacgcatgta
     7621 accaccatat acacgtcaca ctcgcggctt gatctttttg aaaaccctca aatgaaattt
     7681 gctcccggcc tctcccttgg atacacacaa cctgtgccca aaggtcttgg cgtggcgctg
     7741 gatggcgaga tagctggcgt aatcatgggc ggagaacagc aggaatcata cgatttcctc
     7801 tcctatgtgc catctgcact cccatatcta cttcttcaac caaaaaatgt cgtcatagta
     7861 ggggcgaaaa atggcattga cctcctgcaa ccgctttttt ttggtgcttt tcacatttcc
     7921 gtaacagaat atgatgcatc tgttctgcga gcctttgcgt caatccacga tgaatatagc
     7981 cctcgtacag tgtatttctt tcacgggtct gcacgaaggc tcctcaggag cctgcctcag
     8041 gagccagaca tcattttgct atcgagaacg gggttctttc cctctgggaa ttttggtttg
     8101 caggaagact atgacctcac agttgaggca ataacatcgt atattaaggg gctgaaaaaa
     8161 aatgggattc ttttcgtgca gatgtttctt cagccaccac cgcggaatga attacgactt
     8221 gttatgaata ttagagcagc gttgaataag atgggggttg cagggttaag taaacagctc
     8281 ctgatataca ggagctggga tacagtcaac atcctcgtta aaaaggatgg cttttctgaa
     8341 acagacttaa ataagattgg tcaatttttg tcaaaccggc aattcgacat tctttatcct
     8401 ggtatggctg ggcaagaaaa gtttataacc ggtctcgatt atgaggccct atttaataaa
     8461 atcctcgttc atgaatcgtc agccgacttc agttcatcct atgtttttga tatacgggaa
     8521 acaactgatg accgaccatt ctttcattat tatttgaaat taagcaatat aagggagata
     8581 tacagaattt caggaaggaa atggccctac ctcatccacg agggaatgtt tttgcctttt
     8641 gttcttattt tccttgtagt catcgccatt ggtatttttg gatcaaccct gctgtttctg
     8701 aaaagacgtg gagctggaaa tttacaactg aacaccggcc ttttaatcta ttttgcactg
     8761 atcggttttg cgtttatgtt tactgaaatg ttcttcattc atcggctcat actacccctg
     8821 ggatcccctg tcagggcgtt ttcaataacc cttgtgacaa tccttatgag cagcggaata
     8881 ggaagcatgt taaccgtttg gttcattgag aggaaaatga tctggattat aggcctcacc
     8941 cctttgattg ttattgcaaa ctatattatt tttgacataa tcgatgagac tgcgttatca
     9001 gtcttttctg tggtccctat tggcatactg ctcgggttgt tttttccggt tggcctcaga
     9061 tttcttgtga gtaaagaaac agatggagga gtgcccttgg cgtatgcagc aaacggaacg
     9121 gcgtccatta tcgcgccatc actggcttcc cttgtggcag tttcctgtgg ctgcagcatt
     9181 cttctgatcc tggctgcaat tttctatgcc ctggcgattg tcatactgct atttgccaca
     9241 cgcaggatat cctggatgtc caaacatacc tggacatttt tattctgatt ttgttgttat
     9301 tgattactga tcattatgca ccgagaacac cttcaactat ttcttctgtg cctgcccggt
     9361 catagggaca aaggtaactc cgcccaactc tttgattgag tatctgttct tttctttggt
     9421 tacaagtgcc aggttttgat aaaaaaccgt gcttccgagg ggtattatga ggcgtcctcc
     9481 ttctttcagc tgggccagga gtggcggggg aatgtgattg gctgaagctg ttaccataat
     9541 tacatcaaat ggggcataag attcccaccc atagtagccg tccccatgtt ttgtccggat
     9601 gttcctatag ccaagccttt ggaatcgtgt cttggctgtt tcataaaggc tttttttaat
     9661 ctcaatagta tagacctcct ttacaatcgc tgataggatt gcagcctgat atccggaacc
     9721 tgttccaatt tcaagaaccc gttcatttcc ctttagacct accacctctg tcatcagagc
     9781 aacaacatac ggttgtgata tggtctgccc atcacctatt ggcagagggt gatcagcata
     9841 ggctttattc tgtcgacttt catcaacgaa aaggtgtctt ggcactgtaa gcatcgcatt
     9901 aatgacaaga gcgtccttaa cgcccctccc tttaatgtct ttttccacca tctcttggcg
     9961 tcttttatca aaaatatcat caggaaaagc aacactctga caaacaatcg ttgaggcaag
    10021 gaagagcaac gcggtcagtg accatttttt aatatgagcc gtgtatgcag tctctctcat
    10081 ttataaatag tgtacggcaa tggtatactg aagtaaaggc ctctggtgta tacgcctatc
    10141 atctggaatt ttgagtcatg tttcatccct taacaacaac aattttattg cgataatact
    10201 atgtggtttc agtgttaaaa tacaaggagt ataacctggg aggtaagatg tatgctgatc
    10261 aaccagcttg atgaaacagc tatgagggat gttcgtgttg cctgcgccac cctcttttca
    10321 gaagagcttg cttatgatga ttcttttatt gaaaacctga gtatagagac agttgaaaag
    10381 gcatactggc agaaagcaaa acgatgctat catcacctaa gccctcttag ctcccgtagt
    10441 gaagcccagg aagcattaaa cgacgtccgg aactcccttg agaccctcac atcctttctt
    10501 cgaacagaca gccagatggt tgatgaagcc cccagaatag gtaaaattat tgcaataggc
    10561 ggggcaaagg gaggcatagg gaaaagtatc attgttgcca acctgagcgt tctgttggcc
    10621 tccaaagggt tcagagtgac tgctgttgat cttgaccttg gtggagcaaa cctgcatctg
    10681 tgtctaggca acaaggtgtt actgcaaaac aatattaatg actttcttag aaagagagtg
    10741 gactcgcttc aggatatcgc catcaagagc gagtacggtc catacctcat tggcggtgat
    10801 agtgcacagc ttggcgccgc aaatatcgac ttcgggcgga agttaaaact gttgagggca
    10861 gtggaaaatt taaactcgga cttcatcatc ctcgacctgg gcggtgacac ttcatataac
    10921 atcattgatt tcttcttgaa agcagattac ggtattgttg ttactaccag agactctgca
    10981 tcatacatcg gtgcatatca tttcctcaaa gctgctatgt atagaagatt caacaggctc
    11041 tttggccctg aatcaagatt caggatgtat aaaaatcatc agctggaaca gctcattcgc
    11101 gaaatgatga acccagccga tggtggatgg ccgaaatcaa ttaacgattt gttggaaaag
    11161 gtacggcagg aacaacctga ccacttccct tttatgtgtg aagtggtaga aaatttcaat
    11221 tcttatctgg ttgtgaacaa ggtacctaac cgtcttctcc ggaccttcga tgtaaatccc
    11281 ataataacga gaatccagca ggtcacaagg acatggctcg ccaaagaggt cacatatctc
    11341 ggaagcatct cnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11401 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ncttgctgcc
    11461 agaatcactg ttcctaaaac aagaagggca acgatctgtg gccaaaggat gtcgactcca
    11521 acacctttta agaagatccc tctgacaatg ataaggaagt acctcaaagg gctgagatac
    11581 gtcatgtatt ggaacacttc aggcattgtc tctatgggtg tagcaaatcc agaaaggaga
    11641 atgaccggct gaaaaaagaa gaatgttgca gtcattgcct gctgctgtgt tttggagatt
    11701 gtagagatca gaagtccaaa ccctaaaaca gacagaagat atgcacccgt acacacaacg
    11761 aggaggagta cagccccgtt aatgggtact gagaaccaaa acactccgaa gactgtaaca
    11821 agcgccatgt cgaagtatcc aattatggcg gcaggaattg tctttcctaa tatcagctcc
    11881 aaaggtctga tgggcgttac catgagttgt tccatagtac ccacctcccg ctcccgtaca
    11941 actgacatgg atgttagcaa gagacaggtt agcataacga tcaatgctat tacaccagga
    12001 accatgtaat tcctgctctt gaggtcggga ttataccaga ccctgcttcg aagatctagg
    12061 ctccaaacgt tggccttgat cctggcatcc atcatgtccc tggaatactt gccgataatc
    12121 gtgtttacat aactcattgc aatgagtgca gtgttggagt cggttccatc tacgatgacc
    12181 tgtatatcgg tccctatacc tgtttggata tcccttccaa aaccatggtt gatctgtatg
    12241 gcacagagaa catcaccctt gtcgataagt tcccgtacct cttcaggcga cgcaggtaca
    12301 cgtttgacag taaaatatcc tgatgattcg agccttcgta ccaattctct gctttcagag
    12361 gagcgatcaa gatcatagat ggctgtagac acattgttca catccatggt cgccacataa
    12421 ccaaagacta taatctggat gagtggcgtg gcgagaagaa aaaacaccgt cctcttttcc
    12481 cgaaaataat agataaactc tttaatcaca agattcttga ttcgttcaaa catccttaat
    12541 ctatcctttt gcggaacttc ctgttggcag caataaatac caatacagca aagagtgtga
    12601 gcagcaacgc ttctaaccag agaactctca atgtacttcc tttcagaaat atccctttaa
    12661 gaatagtaac gaagtaacgg gcagggataa aatacgtgat aacctgaaga ggcttgggca
    12721 tatttgagat actaaatatg aaccctgaaa gtaagaaggc aggaaggaat gatgtgagga
    12781 gcgcagcctg actcgccaca agctgggttt ttccggcaat tgacatcagc atcccaaagc
    12841 tcagcccacc gaacaagaag atcgctgaaa ggccaaggag cagcgcaatg cttccccgta
    12901 agggaacgtc aaaaagaagg gtgctcatta aaatagtcag gagggtgcct gcaaaaccta
    12961 taagaaagta cgggatcaat ttaccgagaa taagctccgg tgtcttaatt ggggtggaga
    13021 taagttgctc catggtgcct ctatcccatt cccgagcaac tgtcaacgcg ataagaaggg
    13081 cgataatgac agccatgatg atggcaatga gccccggaat aatgaaattt ctggatttaa
    13141 gctcgttgtt gtaccatact cggcttctcg actcgatcaa cggactcatt cgggagcccc
    13201 gcaccctttg gctaaaaccc tcagctatcg ctgttgaata gccctgggca atggtagcgg
    13261 tattggagtc acctccatcg atgagcactt ccacctgaac agggaaacct ttttttatat
    13321 tctttgaaaa atattcaggg ataacaatgg ctacccttgc cctgccggag tcgaggtaag
    13381 ggtcgatggc tttatagctc tcaacataag aattaacgcg gaaatatccg gattcgtgaa
    13441 actgatttac aaattccctg ctcaggctgc ttttatccat gtcaaataca acagtgggga
    13501 tcttgtcgat atcgaaggtt atggcgtaac cataaataaa gaggagaata accggcatga
    13561 tgaatgccat ggcaaggcta agtggatccc gccaaatctg tatgaactcc ttctttgtga
    13621 ttgcttttat tctcagtagt ttcatcgtta tatatttttg ccttcaccta ttacacgtcg
    13681 ctgattacgc atcacggtat ctcacgacac ctcgatcaat gtgacaaaaa catcctccag
    13741 cgaaggcggt atcttttcta ttcctttgac agccactcct gactctgcca atacactcga
    13801 tatacgttcc ctgaatgttt cttcgtcatc aacaactacg tggagtgtac tcccaaagat
    13861 cgcagcctca atcttatgaa aggtcagagt gttgaaggcc tcaacaactt tgtcgacatt
    13921 gacttccagg acgtctcgtg tcatatgttt ccttcttaaa tcagtcggtg taccttctgc
    13981 gacgatcttt ccccggtaaa taagggcaag ccggtcacag taatcggctt catccatgta
    14041 atgtgtcgtg acaaatacag tggttccggc ctgcgacatc ccgttgatga ggttccagaa
    14101 gttccgtcgt gaaataggat caacaccaga ggttggctca tcaagaaaaa ttatatgggg
    14161 atcatgaagt atagcacacc ccagggcgag acgctgtttg aatcctgcag gaagagtacg
    14221 ggttaaagca tgtctctctt ccttcagccc agccatgttc agaacccatt cttttctctc
    14281 aatctttttc tcttttggaa cattgtaaac accgccaaag aagttgatgt tctcttcaac
    14341 ggttaggtta tcgtaaaggg aaaacttttg agacatatac ccgatgacct ttttgataag
    14401 ctcggactcc ttgttgatat caaatcctcc cacagtgcct gttccccctg taggcatgag
    14461 aagaccgcaa agcatcctga tcgttgtaga tttgccagca ccattcggtc cgagaaaacc
    14521 gaatatctct ccccgtcgga cagacaggct aatgttgtct acagcaacaa aatccccaaa
    14581 tgtccgcctg agatcattta cctgaaccgc tatgctgttc ccgttcatat cattatctat
    14641 catcagaaaa cccgtgacgc gtaacgcata atcagtaatc agcctcctta acacctaaca
    14701 ggccagaatt tcgcgtcacg gtcttttccc ctcttcccta tccaccaatg ctatgaacgc
    14761 atcttccagt gaagggacta tctctcggta tccttcaatc tccacaccct gtccacggat
    14821 gacggtaagc acctgatcga caatcccttt ttccttaatg cctacatgga gccggtcacc
    14881 gtacatggaa acattgatga caccatcaat ggtacttata acatcactta ttactttcgc
    14941 ttttgccgaa gaaatctcca ccatgggtat ttgaagactc tctcttatcg aagaaggctc
    15001 atcaactgtc aaaatagttc ctttgtggat taaccctatc cgtgtacaac gttctgcctc
    15061 atcaaggtag gcagtagaga cgaatactgt tatttcctct ttcaagaggt cataaaggat
    15121 tttccagaag tccctcctgg acactgggtc aacaccattt gtcggttcat caagaaacaa
    15181 caccctgggc gtatgtataa gagcgcatgc aaggccaagc ttttgcttca ttcctccgga
    15241 caggtttcct gcaagtcttt ccttgaaagg ggtaagattg ctgaatccaa ggagcctttc
    15301 tactcgcgga ggtctctccc ttgcggggac accataaaga tccgcataaa acatgatgtt
    15361 ttccatgaca gtaagatctt cataaaggcc gaaccgctgg gacatataac ctatttcttc
    15421 ctttattaac tctccttcgg taagaattga gtatcctgca acccatgcat tccctgatgt
    15481 gggctccatt atagctgcca ggagccgcat aatggtagtc tttcctgccc catccggccc
    15541 cacaagaccg aacaactctc ctttttttac ctccaaatta aggttgttaa cggcaacatt
    15601 gccgccaaaa gccttggtaa ggttctcagt tcgtatggct atctcgctat tcattccact
    15661 ttaccgtcgt gatccgtaac gcgtaacgcg tgatcggtaa attaaataaa ccttcaactc
    15721 ttcaagtatt ttataagacc aacaagcatc tttcttatcc gttcgatgtt tgtttcgcat
    15781 ccaatgtttt gaaggaatcc cagcttttct gataataata gttgtgtttc taactccgcc
    15841 aaggaaccca acgagatgta aagaaactgg ataaactcct tctttgagtt ccgtgcggcc
    15901 ccttcggcaa tattacttga aatcgacact gccgatcgtc ttatttgttg ggtgatacca
    15961 tacaattctt ccttaggaaa ttttgaggtc ataagataaa ccagcctggc aagctctata
    16021 gattctttcc agacatccaa atctttatgt gtttttatct tgtccaccat gttttatcct
    16081 cgaagtttct tacccatcac gcgtcactga tcacgcgtca cggttacttt agtgttattc
    16141 tcacatctgc cggcattccc ggcttcagtt ctccattctc gttcttcacc ttgaccttga
    16201 cgccaaagac cagtttgacc ctttcctctt ctgtttggac atttttcggt gtaaattctg
    16261 cttcagatga aataaaggtt actgttccgt catatgtctt atccttgaaa gaatctacct
    16321 ttatatctgc cttttgaccc agtttcacct ggccgagcct gtcttcctta acgtaaactt
    16381 tgacccatgg gttctcaaga tcaccaatgg taaacacagg tgttcccgct gaaagagttt
    16441 caccaagttc cacatttttt cttaaaacca cacctgtgat aggggcaaac agtgttgtgt
    16501 cttttaagcg ctcttccgat gtgtttacca tggcctttgc ttgttgaacc cgatattccg
    16561 cagcctgaat atcttctttt cttggacctt cctgaacaag gcttagagtc tcctgcgcac
    16621 ttctgtactg cgctgttctt gtgttgtatg cgttttgggc agcttcgaac tgagaagttg
    16681 aaatggcgcc attcttgtac aggatatcag ccctttcata gtcttttttt gccttgtcca
    16741 gatccgctgc ctgagcatca acacttgccc tggcacgttc aatctcttga atccttgaac
    16801 ctgactttaa ttcagcaagc cttgccatgg cctcttgaag agttgcctta tgctgcatca
    16861 ccacgctggc aagttcagca ttgtcgagcc tcgcgataag ttgaccggat ctcacttgtt
    16921 ggccctcatc tactgctaat tctataaccc gtcctggcaa tttaaagcca atattggtct
    16981 cagtaacctc aacattgccg gacagattga gggcgttgtt atcgttcttg gatcgaaaat
    17041 gatttattag aaatattgta atgattatta gaattataat tccagcgatg agcaatctct
    17101 tttttttcat ctcccaacct ccttcaatct caaaacaaaa aactaaaatt tattgtttca
    17161 ctctcagcta tgggctgtca gctaataccc tgtctctccc attgctttcc ttaatgatgc
    17221 tctcgcgact tcctcatcaa aaaaagagcg gtagtaatcg gcttcagctc ttaaaaggtc
    17281 tgtctgtgcg tcaaggtaat ccgtatttgt gccggcgcct gtctggtatt tcagcttctc
    17341 gatcctcgcg ttctctcttg cgctctcgat tgcttttcct gcaacctcgg ttcgctccct
    17401 tgcatgtgtg atgtttaagt atgcatcacg gacttctctc gttatagcaa gccttatcaa
    17461 tctctcctct tccagcacct tttgaagctc agcccgctcc cggtttatct ctgcgcttat
    17521 aagccctcca tcaaaaaccg gtatcgacat ccttgcccca aaataccaat tttctttgaa
    17581 gccgaaacta tctccagcct tacctccata ttcgcccccc acatagacat caggaagcct
    17641 tttgccctgt gccatcttaa ccctctcttc agagatcatt ctctttttcc ttacagccct
    17701 gtattcgggc cgctgcacta aagacttcgt aagactttct tcaagagacg gcaacaattg
    17761 tggaggagct ttctgataat cgattacaat gttcttttcc gtatcatcca ttcccatcag
    17821 gtttttcata agctcatagg cgctttcacg gttattttta acggcaagcc tctgttcgac
    17881 tgcatgggcg agctggacat cagtttttaa cagatcaata taaggcacag ctccggtttt
    17941 atagaagacc tcaacgtcac tcttgtgtgc ttcaagctgc ttgactgttc catcagttga
    18001 cacgtagagt ttttcaagtt cagcaatttt gtaaaacacg ctggacatat tgaagatgag
    18061 atcctgtttc gatatgacaa gattctcctg cgcaattcct tttctcagct cagcaacaag
    18121 caccgccctg taaaggcgcc ctcccttaaa aattggcagt ctgagggaac ctcccgcatc
    18181 gtagatattt cggctgtact caggtaagtc cggacccgcc ccaaataatc ctgaaataac
    18241 cggaggggtc aaaggcgcag ggtaacgata tcttgaagcg ctgctgccaa aatcaatcct
    18301 tggcatacgc tcagcccgtg ctgcatctat tccgtatgcc tctgtatcaa tatcttttgc
    18361 agatattctg accctgggat tatttttaag ggcatatgct attgcctcag aaagggtata
    18421 tcgctctcct tgaggtttta aaagattatc atcggttttt atgataatag acggcgtttc
    18481 tccagatttt gatagagaat tctgttgtgc ctgcgaaggt ccgacatgaa caataaagat
    18541 cagagccaga attaaacatg ggataaaacc cataattttc aagcttcccc ctgcagagcg
    18601 tgcagaaaat ttaccactca tttcctctct cctttcatag tctttcgaag gtctttaata
    18661 gccgacaagg aaaaaccagc tatatgatca gcgattgctt ccatttcagt tcttccgaat
    18721 ttctgttcag ggaaaagttt tgcaagggca tgacgagcat aaagaaaaaa aaggcactga
    18781 ctgattacgc tcgcacaaca catatgaacc ctcatagcag gcacttttcc tcctaacagt
    18841 tctccgataa tctcagaaag aaaagcaaaa gtcgggcgca tagtctcttc cacaatcacg
    18901 tcgaaagcac tggttggttc tatatattct cgggacacca accttccgaa aagagaggac
    18961 aggtcatcgc cgagaatacg caaaactaaa gaacgaataa aaactcttag ccgtatttcc
    19021 ggggaatccc ctttttgagt gcccaaatca tgggggtatt ttttgaaagc cagcgcctta
    19081 ctatatttta agacttcgat atacaggttc tctttgtctc tgaaatggta attgatggca
    19141 gctacgttta ctgatgccct tgaacatatc tcccgtactg tggctcgctc aaaccctgaa
    19201 tctgcaaata cctcagcagc agcctggagt atccgttcac gtgtcccagg cgttgattcg
    19261 acattttgat gattcttttt cataatatta aacaatagtt ttaatcaatt aattaaaaca
    19321 attgctttaa tctgtcaaga gttttttatg ctttttacac gttgatagga ccgacatgac
    19381 ggatagggct tcagaacatt aaattaattt atggcttttt aaacaactgg atttataata
    19441 taaaatctat ttctttgaag ggggtgtgat attaatgaga aaaacaaagg tgccagacgt
    19501 ggtcaaaacc aaattgaggc catacttaaa aacagctctt gacaataaaa ttactgacgc
    19561 aattgtaatt aaaacatctg cggtatttac agccccctgg gtacgcataa agtgtcagtt
    19621 cggctgtcca ggatttggtg aaactctctg ctgccctccc cacacaccta ctccggacga
    19681 aatgcgtaag attcttgatt cttatacata cagcattctc cttcacctcc actggacaaa
    19741 gaattacagg atagccaata aattcaatga tatattggtt tatttggaac gtatgatatt
    19801 tcttgacggt tattacaagg cctgggctct tgggagcgga ccttgtgaca ggtgcaagac
    19861 ctgcaatatc gatgggacat gccgcaacgc tgacaaggca aggccttcaa tggaaggatg
    19921 tggcattgat gttttcaaaa cagcaagaga gcaaggtctt ccaattaatg ttgtccaaaa
    19981 ccacggcgca gaaagggatc tttatggcct tgtactcgta gagtagagat tgttcttttc
    20041 aaacagagga ggattatgat tttgttactt ctgacgcatg ctatctcatg atcttagcgc
    20101 tatgtataat gcgtgctggt ggcataatta cgcatttcat gaaaaaaagt ctgggctgag
    20161 agcaccgatc cctcggtata tctggagcaa ttttcttcaa ctttgccata ttggcaattg
    20221 tcgtccagat aacgctcgcc gagagagaac atttcaggat cattcactcg tagataggtt
    20281 ttgatgcctt tgtttttgct atgctggcac ctatattggg aatacaccag ttgaaagttc
    20341 gaagcgtgac cgtcagattg cgaagtttcc atcgctggtc tggaaggctt acacaatgcc
    20401 tgatgttgat taatgttttt ctcggttctc catgatgggc tttatattga acgaatgtat
    20461 aatcctaaac aatcttgtaa aaaccgttct cttcacaaat aaacccttca tgaataaggc
    20521 ccctgagtat tcttcccaga tggtcaaggt taatgccaat tgattcatta aggtctttca
    20581 cagaaagatt gctttcatca agaagtttct ttattattaa gccacgtatc tgccggtcag
    20641 atcctgcaaa ggaaggttgt cttacatagt gagcgcttct cctgtttgga ttttccgttt
    20701 ttctcttaag catggaccca tagtccatga gtgcccagta ccattcacgg ggattttccc
    20761 ggtccatcgt tgcttcgaca agagggaata tctccccatc cttcacatct tcctttttac
    20821 gaaagaattc atggataaaa accctccgga tattcgtttc aatgaaaaca gacggcatat
    20881 taaaagcaaa agccagtatt gctccggctg tagtttttcc aataccagga aggctgctga
    20941 gtttgttcac gtcctggggg agcaccccac catacacttc aacgatctcc agggcaagct
    21001 tcttcaatgc aagagctcgc ctgttgtagc cgagtccctg ccacatggca agcacatctc
    21061 cttgagaggc ttcagccaga acattgaaat ttgaaaacat ctttataaac tccaggtatt
    21121 tgtcatgcac gcgctctacc tgcgtctgct gaagcatgat ttccgatacc agtatattgt
    21181 aaggatctgt tgtgcgccgc cagggaaaat cacgaccatg atgtttgtag tactcataga
    21241 tttgccgctg aaatgccctt atttcctctg tggtgagaat ttttcctttc attgttaaag
    21301 ttatacgtcc tttgttttgc gttatacagg taaagaattg tatattaaca caagacttat
    21361 cttccatcga gggttctatg catgcgtctg agactccaag aaaattagtc agtttcctgc
    21421 ttgatcccca aagttatcca gaaaataccg ccaatgttac acatattgaa acccatatat
    21481 cccatgtttt tctctgcgat tcttttgttt acaagattaa aaaaccggtt aatttcggtt
    21541 ttcttgattt ttcatccttg aaaaaaaggt attacttctg caggaaagaa gtcgaactga
    21601 acgctcgcct tgccaaaggc atttatttag gtgttcagcc aatctacaat gctgatggtt
    21661 gctattcgtt tgtaaacaac aaagaaggct ctccggtcga atttgccgtg cagatgaaac
    21721 gcattcctat ggagtgcctt ctcttcaacc ttatcgaaaa aggaagaccc ctctatcgcg
    21781 atctggaaga agtgggcaga acgcttgcat tttttcatac taatatcaaa ccctataggg
    21841 gcaaaagatt tggtggtttc gaggcaatac tcgctgctac agaagacaat tttgagcaga
    21901 tcaagcagtt ctgcggaatt actgtcaatg agaacacttt tgaaaccatt gccagctaca
    21961 caagagaatt tcttgagaaa aataagaata aattttacag ccgcaaacaa aatggattca
    22021 ttcgagaagg ccatggtgac cttcactgtc agcatatatg tcttgagcat ccacctgtaa
    22081 tttttgattg tatcgaattc aacgaggcat tcagaatcat cgacgtcctt gaggacattg
    22141 catttctttt tatggatctg gagtaccgcg gaagattcga tctgacatcg tgcctttcaa
    22201 aagcatattt tgccgatctg caaaaagtgc aggatgagga cttgctcagg ttctacaaag
    22261 tgtatcgtgc tgtggtgaga ggaaaggttg agggattgac agcagaaaat gtgacaacgg
    22321 caaaagagca aaagctgtca acggcaaagg cgatgaatta ttttgatctt gcctgttact
    22381 atatcaatga tgctcataag gccttcaatc ctatcgtatt catgggtcta tcagggtcag
    22441 gaaaatcgac catcgccatg ggcttttcat cggacgctga cattttacat tccgacgaaa
    22501 tacgaaaagc agttgctggt ctgggaaaaa atgaacatca ctatattgaa gtaggaaaag
    22561 gaatatacac accggagctg actggacaga tttattatac cctcctggac aaggcagtgg
    22621 gctgtgcaaa ggagggcaaa aaggtcattg ttgatgctac ctacttgaag gcaaatcagc
    22681 ggacccattt ctacagctca tgcattgaga caggactgaa tccattcttc gtacactgct
    22741 ttgcatcaga gaacatcctc agaaagcgga ttattgaaag aatgcaaaat ggccgggata
    22801 tttcggatgc ccatctgagt gtccttgagc atcagttgaa acatctcgaa gaacctgaag
    22861 agttgcccag ttttcgtgtt ctgaaaatta atactgagga tgctatcgag aatattgtca
    22921 acacattgaa agaatttctt taagagttgt actgttcaca aaaaaaaagc gggcctgccg
    22981 gcccgcctgt tatcttacct tatttctttt tcgcttttac tgctttcttg gcaggggctt
    23041 tcttagcctt tgctttcact ggtgctgtct tgccagttgc tgctgccttt aaaactgctc
    23101 ctgccgaaaa cttggggacc ttctttgcag caatcttgat ctctttacct gtctggggat
    23161 ttcttccctt tctggccttt cttgcagcaa cagaaaatgt tccaaaacct ataagggtca
    23221 cttttccacc ttttcttatt gtgtctgtaa tggacccggt aacaacggcc agcgcccttg
    23281 ccgcagcggc tttggaaatc ttaatttctg ctgctactct gtcaacgatt tctgcctttg
    23341 tcatcctgat acctcctcat gcatgtgtat gaata
//
LOCUS       DAOOMC010000031        22808 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdus sp. isolate AMR_MDS_2685
            NODE_2160_length_22808_cov_6.231706, whole genome shotgun sequence.
ACCESSION   DAOOMC010000031 DAOOMC010000000
VERSION     DAOOMC010000031.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864760
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdus sp. (wastewater metagenome)
  ORGANISM  Syntrophorhabdus sp.
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae; Syntrophorhabdus.
REFERENCE   1  (bases 1 to 22808)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22808)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,729
            Genes (coding)                    :: 2,708
            CDSs (with protein)               :: 2,708
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 7 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22808
                     /organism="Syntrophorhabdus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2160_length_22808_cov_6.231706"
                     /isolate="AMR_MDS_2685"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2699756"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            108..1526
                     /locus_tag="PK125_04690"
     CDS             108..1526
                     /locus_tag="PK125_04690"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: protein motif:HMM:NF003274.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HPB37439.1"
                     /translation="MIGITSIGAYIPRYRLTLEEIGKMWQVRGGPGEKSVAGYDEDAV
                     TMAVAAALDCMKLNNNQADALYLATTTAPYKEKQSAALVAGAADLEKTAFTADFTNTL
                     RAGTTAIKAAVDAIKSGSARNALVVASDCRIGAPMGRFEQLLGDGAAAIMIGTKEPIA
                     MIEGVYSIFSDFTDYWRTERDSFIQSGEGRFLEEEGYMPIMTEAIHELMRKYSLRQKD
                     FNKAVFYAADNRNHASLAKALGFDKSQVQDSFFTKIGNTGTAAAMIMLVAALEEANPG
                     DRILFAGYGDGADACILRITENISRIKGTPVMEDRFARSVSIGYGKYLLWRDLIPVEA
                     SSLPERTEPSLATRWREHKTVRALYGVKCRRCGAPQIHPNGQRVRVCIACQEKDDFED
                     YKFSDKTGKLFTYAIDQLQPTKNPPGLNGVVDFDGGGRLICELTDYDLDNVETGMTVE
                     MTFRKLYQGKGIVNYFWKAKPI"
     gene            1584..2774
                     /locus_tag="PK125_04695"
     CDS             1584..2774
                     /locus_tag="PK125_04695"
                     /inference="COORDINATES: protein motif:HMM:NF004810.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA acetyltransferase"
                     /protein_id="HPB37440.1"
                     /translation="MESIKDRVAIVGMGCTRFGERWDMGVEDLLVEAAYEAFEDAGIE
                     PKDIQAAWLGTVFSGISAMTLAPLGLQYIPITRVENMCATGSEALRGAAYAIAAGVCD
                     IALAIGVEKLKDSGQTGVKGPAIVGDNPDGSSAIGSGYSAPASFAYLGTRYFSHYNID
                     RDEGKRMLAEIAVKNHQHGSLNPKAHFHNILTVEQVMKAPIVAWPLGLLDCCGVSDGA
                     AAAIVVRSDMAKSFRGDPIYIKGMAISAGARQGSMRQDYDYVHIDENVRASQQVYAQA
                     GVKNPRKEISCAEVHDCFTAHELIVYEDLGFSPRGGAREDIFGGTFTLKGELPINTDG
                     GLKCFGHPLGASGLRMLYEMYLQLQGKAGPRQVKNPRLAMTHNLGGNAGAGIGCCVIV
                     GNEK"
     gene            2797..3390
                     /locus_tag="PK125_04700"
     CDS             2797..3390
                     /locus_tag="PK125_04700"
                     /inference="COORDINATES: protein motif:HMM:NF013607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB37441.1"
                     /translation="MNHPHHLVFVCIGNRNRSPFAEFFFSKLISQRDKQLVDRISVIS
                     AGFMPQRMKDKMAILKIGAPNPFFGRTLPQATRTVLLRHGIKVPDVWRSQELTPELVD
                     RAELIITALPDQKNDIARLYPKVADIIFTIREISQWEGYLLQEDYDFNKIPRDGSLWS
                     YVEENFDYVANILSEIEKMLIKAYPNIMIRMGFSKNH"
     gene            3471..4127
                     /locus_tag="PK125_04705"
     CDS             3471..4127
                     /locus_tag="PK125_04705"
                     /inference="COORDINATES: protein motif:HMM:NF021561.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2284 domain-containing protein"
                     /protein_id="HPB37442.1"
                     /translation="MNPKKGPSRRIVLHITEEVLEEDLNQLKQKALDLGASMAEIIPA
                     SWIDVDERVILKCRVPLCPYFGRSPHCPPNNPHPDFMRQAFGKYTWAILFAIDVIPVA
                     EFSNRVVEQKAGVEWTKRNMEITGTLESTAFGFGYYLAMGFSQFNCHVALCEGKPCQV
                     LQGNKCAYPLKARPSMEGVGIDVFRLVTKAGWEIYPIYRSVDPAVVPRAMSVGIVFVC
                     "
     gene            4318..4602
                     /locus_tag="PK125_04710"
     CDS             4318..4602
                     /locus_tag="PK125_04710"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HPB37443.1"
                     /translation="MSKRIFRFVGKEGVVIFRHNEKVVIVTGGVSGIGFATGRLFAQQ
                     GAKVLLVDLQKDSLCKAVEKIGLLSVSYAMADVPLRSMEGSIAIVFSIAR"
     gene            4599..5558
                     /locus_tag="PK125_04715"
     CDS             4599..5558
                     /locus_tag="PK125_04715"
                     /inference="COORDINATES: protein motif:HMM:NF013053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HPB37444.1"
                     /translation="MKGSAGTSADCAGKNGVEGIMQAAAIECAPFGIRVNTVHPGLIT
                     TRVAEGHVRVCYYDTPEEGKKAPAESAPLKRYGSLEQEVALMLYLVSDESRYCTHGII
                     RLMVEKGLCNEEVIMDMSNLRSKVAPERSALIIIDMQKDYCSEGGIFHRMGYDIKSAK
                     ALAARLNDFLGHARKVLKNIIHVKMTKIDSLSSSTALEQYRRLGVNRQYDPAFSEFYE
                     VLPQEGEVVIPKYRHSPFVSTYFDQLLRIRQIQTLIVTGLATNVCVESTARDGFARDY
                     HVVIPEDLTEGTSLEAKKWSLSNINLFFGEIVQSQDLLRCWDL"
     gene            5743..6312
                     /locus_tag="PK125_04720"
     CDS             5743..6312
                     /locus_tag="PK125_04720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB37445.1"
                     /translation="MSKIYIDKRYFTDKKAKWVSFEDTQGLRETKKDIYGKCVPCITN
                     LYEQLKDGKTIVDLGFALKCWKVVVVLNSMEECVDLLSELEISMTEGIKVKGRFGSVN
                     ETKTTKVIVFNVAGEAQRRKLYEILKDCVKRVNPHAEISYHRGCVELYHELFGDWKRW
                     KKFTLVQKPEAIEPIIRKIREVLFWDKPT"
     gene            6605..7765
                     /locus_tag="PK125_04725"
     CDS             6605..7765
                     /locus_tag="PK125_04725"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPB37446.1"
                     /translation="MKYRSAALLTLAHAATDINQGAIPVLLPFFIAQHNLTYAAAAVI
                     VFTTTLVSTITQPVFGIVADRLSRPWLMPFGVLLAGVGLSCVGLAPSYETGLIAVALS
                     GIGVAAFHPEGARLANRFAGDKKATIMSIFATGGQLGFAIGPLIASFALIRWGIKGTA
                     FLAVPAIGVTLLIALILPGLWRYHSVDGGKAVKKSQGEDAWAGFLCLSILVLFRSVIF
                     YGINTFLPLFWINVLQQSKTAGASALTVFFASAVVGNLIGGRVADHVGFRRAILTEFA
                     LVTVLLPIFAYTSNPLWAMFLLIPIGLMFSASVGPLVVLGQSYLPNRIGLASGVTLGL
                     AFSFGGITTPVFGWIGDRHGLHTAIVVLSFLPIICTGLTILLPKPKAAITAK"
     gene            7790..9355
                     /locus_tag="PK125_04730"
     CDS             7790..9355
                     /locus_tag="PK125_04730"
                     /inference="COORDINATES: protein motif:HMM:NF015078.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB37447.1"
                     /translation="MFITESRGIWRWFTRDFLGIIHEMRWSYIPPLMVYLAAGISGFT
                     GIIESFFVKEELGLSAAFLAGLGFWAGLPWALKMPFGHLVDLYWHHKSLFVYLGAALM
                     AVSLAIMIGLTGHKPFMAQIMALDTWYVIATLVSPVGFVLQDVVADAMTVEAVPSYDE
                     NGVKIPQDEFERMHVTMQTLGRIAIIGGSAIVAGVGGWLAMSMSYYAMYKLSLVIPVI
                     SVLGAVIGAWNVRRLRVHYRRSGCSEKEIHELLRSETCDIKPSWYILGGGIVFVIVSI
                     ILGMSQLAAKKEVVFVSSLAIIIYLMHQLLKDLDPAKRRDIIGIAIIVFVFRAMPGIG
                     AGASWWQIDVLKFDEAFFGTLRQISSILAIIGMFALRGWMSRRPIPYLVVFLSAYATL
                     MIMPFLGMYYGLHEWTERVFGFGARTIAIIDTMADSPMGQVSMIPMLAWIAREAPLEQ
                     KATYFAVMAAFTNLALSASSLGTNYLNEIFVVGRGQYEELGRLMICATILGLVLPIVT
                     VILVNWRNRSMCK"
     gene            complement(9545..11245)
                     /locus_tag="PK125_04735"
     CDS             complement(9545..11245)
                     /locus_tag="PK125_04735"
                     /inference="COORDINATES: protein motif:HMM:NF014325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-binding protein"
                     /protein_id="HPB37448.1"
                     /translation="MKFYSSIVSYLVEGKSTKQNMRMLIRFLVMLTAMITVYSIIFHF
                     LMAWEEREYSWVTGFYWTLTVMSTLGFGDITFASDAGRLFSILVLLSGVIFMLVLLPF
                     AFIKFFLAPWMESEAKRRAPREVSPDTKDHVIMTAYDDVTAALVERLVTYKRDYVVIV
                     EKPEHAGLLSDRGVKAAVGNIDDPDTYKRMRVHDAALVVATNSDEVNTNIAFTVREMN
                     ESIPIITTADSPHSVDILSMAGSSRVVELPDLLARSFANWTMCGNFQANIIGRFDELV
                     IAETPVINTPLVGKTIAESNLRESVGVTIVGIWERGKPSVPTPKTQITRSTVLLLAGT
                     ESQIASYDDVYSIYQMFQHAGDPVIIMGGGRVGTAIGRRFAERDVPFLIIEKNPKKTS
                     ESANIVYGDAADLSTLKRAWIEKAPAALITTHEDATNIYLTKYFRSLRPDLQIISRAN
                     LDRNVSTLHRAGADFVMSYPVLGVDAVFSFLTKQDVLMLVEGLTLFRVQAPEILDGVT
                     LADSRIRQETHCSVVAIKSDGNFIVNPGPCIPVTKGSELILIGTYEGERQFFRTYIKT
                     "
     gene            complement(11445..12344)
                     /gene="zupT"
                     /locus_tag="PK125_04740"
     CDS             complement(11445..12344)
                     /gene="zupT"
                     /locus_tag="PK125_04740"
                     /inference="COORDINATES: protein motif:HMM:NF003243.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZupT"
                     /protein_id="HPB37449.1"
                     /translation="MKNVWIAFGLTLFAGISTGIGSIIAFTAKRTDYRFLSVATGFSA
                     GVMLYLSFVEIFPMGFEALSKQYGEYWGHWINAAGFFGGILLIGLIDNLTPATENPHE
                     IHTEAETMPLRNPTAPLPVFDQPATRSERTENSSHPAIAHQKLLRTGLFTALAIAIHN
                     FPEGFVTFLAALEDPSLGVAIAVAVALHNIPEGISVSVPIFYASGNRKKAFTYSLLSG
                     LAEPVGALIGYIVLRFLLGNEGGAIPSHIMGLLFGGVAGIMVYISLDELLPTSRAYGK
                     GHDSLFGLVAGMLVMALSLLVMK"
     gene            complement(12543..13016)
                     /locus_tag="PK125_04745"
     CDS             complement(12543..13016)
                     /locus_tag="PK125_04745"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HPB37450.1"
                     /translation="MSDSILNGKRILAVDDEPDILSVLEEEIMDACPDCIFDRAITYE
                     SAVKLLESKPYDLVILDIMGVRGFDLLDLAVKKDLKVAMLTAHALSPETLNKSIEMGA
                     RAYLPKDKLGEVVPFLEDILKYDYETGWKRLMDKLQGFFKDRFKDDWEVKTWISK"
     gene            complement(13404..13634)
                     /locus_tag="PK125_04750"
     CDS             complement(13404..13634)
                     /locus_tag="PK125_04750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB37451.1"
                     /translation="MNRDDERMAFESIINDFITHYKNGPEMFASRANFDAFVNTEVRF
                     HAKLLKVDFEKAKEALLAWVQQNHALEYSELI"
     gene            complement(14201..14521)
                     /locus_tag="PK125_04755"
     CDS             complement(14201..14521)
                     /locus_tag="PK125_04755"
                     /inference="COORDINATES: protein motif:HMM:NF013089.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HPB37452.1"
                     /translation="MSWMFLLIAGVFEIGWPLGLKLSQTTANKVTWILVSIVSMTLSG
                     LFLWVAQKSIPIGTAYAVWTGIGAAGTFLIGIAFFEDPANTPRILSVILIVLGIIGLR
                     LYQV"
     gene            complement(14760..15761)
                     /locus_tag="PK125_04760"
     CDS             complement(14760..15761)
                     /locus_tag="PK125_04760"
                     /inference="COORDINATES: protein motif:HMM:NF013921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HPB37453.1"
                     /translation="MPRKARIDAPGALHHIIVRGIEQGIIFQDHTDYDSFVQRLATVL
                     NETATQCYAWTLMPNHFHLLLRTGSTPVGRMMQKLLTGYAVTYNRRHIRSGHLFQNRY
                     KSILCQQEPYFLELVRYIHLNPIRTGIATNMNELARFAYCGHGVLTGKRQNNWQNTSE
                     VLNLFATDLDAARAKYHEFIAKGLPMGHRNDLTGGGLIRSSGGWKQVIAKRRANEYQK
                     SDERMLGDHEFVCHALATANENSDWRYSLKERGIDLTAVISRVCEVLHCNRCDIFTPG
                     KIQSRVNARSLFCYWTVRELGTNQAELSRRLRLSPAAITQSVKRGESIVKERGYSLV"
     gene            15899..16096
                     /locus_tag="PK125_04765"
     CDS             15899..16096
                     /locus_tag="PK125_04765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB37454.1"
                     /translation="MEEIAKISIDEYERRPDGSWVCIKNSDITTKSNWVIRVSPGVIF
                     QKNRRLFGLNVADALDKISGN"
     gene            complement(16284..17000)
                     /locus_tag="PK125_04770"
     CDS             complement(16284..17000)
                     /locus_tag="PK125_04770"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HPB37455.1"
                     /translation="MKKAIVIGGSSGIGRELAKILSENGYIVGIMARRVQLLEELRKD
                     VEGILFVQKIDVANVEVAMETLNEFLKEMGGVDLIVISAGTGDINDSLSWHIENETIK
                     TNVNGFAALINVIMHHFTEKGSGHLVGISSVAALRGGRESPAYNASKAFESNYLDGLR
                     QKVKKSGLPITITDIKPGFVNTAMAKGDGIFWAAEPGKAARQIYHAIKSKKRSVYITR
                     RWLMVAWLIKLLPGFIYDRL"
     gene            complement(17052..18485)
                     /locus_tag="PK125_04775"
     CDS             complement(17052..18485)
                     /locus_tag="PK125_04775"
                     /inference="COORDINATES: protein motif:HMM:NF027964.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase NADP-binding
                     domain-containing protein"
                     /protein_id="HPB37456.1"
                     /translation="MNKHLTTPFFEGRVIMIGCGSIGQAVLPVIGRHLEGIHDRLIVL
                     SADESGRRIAERCGAKFIHCNLTPGNYRSILKNYVSDGDLMLNLSIDVSSIDLIRFCA
                     VRGALYVDTSIEPWPGVFDNPMLELHQRTNYAIRAKIIDLAAELGPDSPTAVVDHGAN
                     PGLVSHFVKRALVDLDQIVRNGNTKPSSREEWANLAHDLGISTIQISERDTQASQRPK
                     RHGEFINTWSIDGFVDELMQPAELSFGVFEKRKPKGAREHRRGSGTLYLERPGASTFA
                     RSWAPSIGGFQGMLVTHDEVFSIADYLSIRDGSKFLYRPSVMFVYHPCDDAMLSALEL
                     EGRGWILQPSCRRLGTDIIEGMDELGVLLAGHERNAYWYGSQLLIQEARRHVPFANST
                     TVQVMAGALAAVLWAVRHPRRGVVEPEDLDFEECLETATPYLGRMVGEFTEWTPLDGR
                     GKYFPERLDIQSPWQLQNIRSRQWYGE"
     gene            complement(18685..18933)
                     /locus_tag="PK125_04780"
     CDS             complement(18685..18933)
                     /locus_tag="PK125_04780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB37457.1"
                     /translation="MVNTDRSTNLPGYNGAMNGFIADCGHRLAGISLFNAVLEHEKDH
                     IENFGDKRSGDDPQGVLDLELGGYKRERTASKRFGNYA"
     gene            complement(19211..19441)
                     /locus_tag="PK125_04785"
     CDS             complement(19211..19441)
                     /locus_tag="PK125_04785"
                     /inference="COORDINATES: protein motif:HMM:NF022969.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexameric tyrosine-coordinated heme protein"
                     /protein_id="HPB37458.1"
                     /translation="MADTWLTTLITDTPQAGFELAITLSRRGVKYTQPNAEVLQKLRP
                     VYSNNADSLIAASQVVAINFQTIAAANNYWRK"
     gene            complement(19668..20522)
                     /locus_tag="PK125_04790"
     CDS             complement(19668..20522)
                     /locus_tag="PK125_04790"
                     /inference="COORDINATES: protein motif:HMM:NF040157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5714 domain-containing protein"
                     /protein_id="HPB37459.1"
                     /translation="MKKDHSEEMRPSNAGVIPENIDGEQPCCSPDEEKTCCSPGMEIT
                     GRDSLQHIDIYKSGCAVCGLPLIYFWKNRESTCFFCGQVLQANAACPYGHFVCDHCHS
                     ADAIEIIKTVCLHSRQTDMAELMQTIRSHHLFRMHGPEHHSMVPAVILTALRNSGYPV
                     INEEIQTAIERGKTVSGGACAFLGACGAAIGAGIAISIILKASPYDGEKRQAVLQVTQ
                     AVLARIAAYKAPRCCQRDSWLTLKEITAHIQKQTGKSLKVDTFACDQFLQNKECIHEQ
                     CPLWSPGK"
     gene            complement(20550..21686)
                     /locus_tag="PK125_04795"
     CDS             complement(20550..21686)
                     /locus_tag="PK125_04795"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HPB37460.1"
                     /translation="MEGWGNYQAPVNEDRPPDCPDSCGLCPGHLQKTCCVLIEVTTRC
                     NLKCPVCFAQSGGTTQDPSISEIMDNFEKLVRNGNTFIQLSGGEPTVRNDLPEIVAAA
                     KAAGCENIQLNSNGIRLGQDKGFTRALAQAGLSFVFMQFDGTEDVIYEKLRGRPLFAE
                     KQAAIESCSDEMIGVTLAPTLVPGVNDHNIGDILRFGFEHSPAVRGVHFQPVSYFGRY
                     PRPPENKDRITLPEVLCAIERQTMGRVNISDFLPSACDHPRCGFHGDFVVLPEGLMKL
                     TPKNQTNACCQPDGTEHLKSRNFVSRRWRRAATQSTEQYFDNDGFIYLDAFLDRVRTH
                     GFTITAMAFQDAYTLDVERLRRCSLHVYKDGKTIPFCVRYLTKC"
     gene            complement(22154..22288)
                     /locus_tag="PK125_04800"
     CDS             complement(22154..22288)
                     /locus_tag="PK125_04800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB37461.1"
                     /translation="MEENIMACVGVNTGNFTAYEGSVALGHNGIDRTICHSDIFVEGV
                     "
     gene            complement(22302..22484)
                     /locus_tag="PK125_04805"
     CDS             complement(22302..22484)
                     /locus_tag="PK125_04805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB37462.1"
                     /translation="MLSRIVLTFALSQGAIIRAILFLVILPICAKLPIDTTPPPALET
                     ISTAVKTKLFTFGKAA"
BASE COUNT         6038 a         5031 c         5436 g         6303 t
ORIGIN      
        1 accccgaata catgggacag agaaaaggca ggggttgagg tgaagaggta ggggaaaaca
       61 cgaaaaatgt cttcgaactc tttgaggtaa ggatcaaaga gggcagtatg ataggaatta
      121 catcaattgg agcttatata cccaggtaca gactcaccct ggaagaaatt ggtaagatgt
      181 ggcaggtgag gggcggccct ggggaaaaat cagtagcagg ctatgatgaa gatgcagtaa
      241 ccatggcagt tgccgcagcg cttgactgta tgaagctaaa caacaatcag gcagatgctc
      301 tttaccttgc caccactact gcgccgtaca aagaaaaaca gagcgctgcc cttgtggccg
      361 gtgcggctga tcttgagaaa acagcattta cagcagattt cacaaacact ttgagggcag
      421 gcacaacagc aataaaagct gcagtcgatg cgattaagag tggttcggcc cgcaatgccc
      481 tggttgttgc ttcagattgt cgcataggcg cacccatggg gagattcgaa cagttactgg
      541 gagacggtgc agcagccatt atgattggca ctaaagaacc tattgcaatg attgaagggg
      601 tgtattcaat cttcagcgat tttacggatt attggcgaac ggagagagac agtttcatac
      661 aatccgggga agggcgcttt ctcgaagaag aaggttacat gcccattatg acggaagcta
      721 ttcatgagct catgagaaag tattctctca ggcaaaaaga ttttaataaa gctgtctttt
      781 atgcagctga caataggaat catgcaagcc ttgcaaaagc ccttggtttt gataagagtc
      841 aggtgcagga ttcgtttttc acaaagattg gcaataccgg aactgcagca gcaatgataa
      901 tgctcgttgc tgccttagag gaggcaaacc caggagaccg tatattattt gccggctatg
      961 gagatggcgc tgatgcctgc atattgcgta ttacagaaaa catatccagg attaaaggta
     1021 cacctgttat ggaagatagg tttgcacgat cagtatccat aggttacgga aaatatctct
     1081 tgtggcgtga tctgattcct gttgaggctt cgagcctgcc tgaaaggact gagccttctc
     1141 tggcaacgcg ctggagagaa cacaaaaccg taagagctct ctatggggta aagtgcagga
     1201 gatgcggggc ccctcagatt cacccaaacg ggcagagggt acgtgtctgt attgcctgtc
     1261 aggagaaaga tgactttgaa gattacaagt tttccgataa gacaggcaaa cttttcacgt
     1321 atgctataga tcagttacag cccacaaaaa atccaccagg tttgaatggt gttgtcgatt
     1381 tcgatggcgg gggacgactg atctgtgaac ttaccgacta tgaccttgac aatgttgaaa
     1441 caggtatgac cgttgagatg acttttagga agttgtacca gggtaagggt attgtcaatt
     1501 atttctggaa agcaaagcca atctaaagaa ttgtagtagg ccttatatga tctaaagggc
     1561 ctgtattaaa tagggtcaga aacatggaaa gcatcaaaga cagggtggca atcgttggca
     1621 tgggatgcac tcgatttgga gaacgatggg acatgggtgt agaagacctt cttgtggaag
     1681 ccgcctatga ggcatttgag gatgctggca tagaaccaaa ggatatccag gcagcatggc
     1741 ttggtacagt tttttccgga atcagcgcca tgaccctggc acccttggga ttgcagtata
     1801 taccaatcac gagagttgag aatatgtgtg ccaccggttc ggaagctctg cgcggtgcgg
     1861 cttatgccat agctgcagga gtctgtgata tagcgttagc aatcggcgtt gagaagctta
     1921 aggattcagg acagacggga gtaaaaggcc ctgcgattgt gggtgacaat ccagatggta
     1981 gctcggccat tggctcgggc tatagtgcgc cagcctcatt tgcatatctc gggacaagat
     2041 atttcagcca ctacaacatt gatcgtgacg aaggcaaaag gatgctagcg gaaatagcag
     2101 ttaagaacca tcagcacgga agcctgaacc caaaggcgca tttccataat atcttaactg
     2161 tggaacaggt tatgaaagcg cctattgtgg cctggccgct tggtctgctt gattgctgcg
     2221 gtgtcagtga cggagcggca gcggcgattg tcgtaaggtc tgatatggca aagagtttca
     2281 ggggtgatcc tatttacatc aaggggatgg ctatcagcgc cggggcacgt cagggatcga
     2341 tgagacagga ctatgattat gtccacatcg atgaaaacgt ccgtgcctct cagcaggtct
     2401 atgcccaggc aggcgtaaaa aacccgagaa aagagatcag ttgcgctgag gtgcacgact
     2461 gttttacagc tcatgagctg atagtatatg aagatttggg attcagcccc aggggtggag
     2521 cccgtgaaga catctttgga ggaaccttta cccttaaagg tgaactacct attaacaccg
     2581 acggtggact aaagtgcttt ggccatccgt tgggggccag tggtttgcga atgttgtatg
     2641 aaatgtactt acagcttcag ggtaaggccg ggccgcgtca ggtgaagaac cctcgtcttg
     2701 caatgacgca caaccttggc ggtaacgccg gtgctggcat tgggtgctgt gtaatagtag
     2761 gtaacgaaaa gtaaacatga tgaggggaga caaagagtga atcaccctca ccatctggtc
     2821 ttcgtgtgta taggtaacag gaacaggagt ccttttgcag agtttttctt ctccaagctc
     2881 atcagtcaaa gagataagca gcttgtagac agaataagtg tcatatcagc tggatttatg
     2941 ccgcaaagaa tgaaagataa gatggctatc ctgaagatag gtgcaccaaa tccttttttt
     3001 ggcaggactc tgccacaagc gacacggaca gttttgctcc gccacggcat aaaggtacca
     3061 gatgtttgga gatcccagga actaacccct gaattggtag atcgagctga actgattatt
     3121 acggcactgc cggatcagaa aaatgacatt gcaaggctct atccaaaggt ggctgatata
     3181 atatttacaa ttcgtgaaat atcacagtgg gaaggctatt tactccagga agactatgac
     3241 tttaacaaga tccctcgtga tggttcgctt tggagctatg ttgaagaaaa ttttgattat
     3301 gttgcaaata tactctcaga aatagagaag atgttaatca aggcttatcc aaacatcatg
     3361 ataagaatgg gtttctccaa aaatcattaa atctatggga aacgaaaaaa atcgaatatt
     3421 attatgtagg gctgaattgg attagcagcc agtcgaagga gggtgagaat ttgaatccca
     3481 agaaggggcc gtcccggcgt attgttcttc atataaccga agaagtactt gaagaagatt
     3541 tgaaccaact gaaacaaaag gcactcgacc taggcgcttc tatggctgaa ataatacccg
     3601 catcatggat cgatgtggat gaaagggtaa tattgaagtg ccgggtacct ttatgccctt
     3661 actttggtag aagtccccat tgtcctccaa acaaccctca tcctgacttc atgcgacaag
     3721 cttttggcaa gtatacatgg gccatcctct ttgccatcga tgtaatacct gttgcggagt
     3781 tctcaaaccg cgttgtggag cagaaagcag gagtagaatg gaccaaaaga aatatggaaa
     3841 ttactggcac tttagaatcg actgcttttg gtttcggata ctatctagcc atgggttttt
     3901 cccagttcaa ctgccatgtg gccctctgtg agggaaaacc gtgccaggtt ctgcaaggta
     3961 acaaatgtgc atatccactg aaggctcgac cttctatgga aggggtaggc attgatgtat
     4021 ttcgtctggt gacaaaggct ggatgggaaa tctaccccat atacagaagt gttgatccgg
     4081 cagttgtacc gagggctatg tctgtcggaa tagtatttgt gtgctagggc ggtgggttct
     4141 gggatttgaa ttgaccggag acgaccatct actcccttaa ttacggggca gagacaaata
     4201 ggctgccatt gactgggtta caaccgatgt tttggattat acaaaaagat aatcagattg
     4261 gggcgattgg tatgatttca attggaatag gggatcctgc atagaaatag accctgtatg
     4321 agcaaaagaa tttttcggtt tgtcggaaag gaaggggttg ttatattccg gcataatgaa
     4381 aaagtggtga ttgtcacagg cggtgtatct ggtattgggt ttgctacagg taggctcttt
     4441 gcacagcagg gagctaaagt attgctggtg gaccttcaga aagacagcct atgcaaggct
     4501 gtggaaaaaa ttggattgct atctgtaagt tatgctatgg ccgatgttcc attgagaagc
     4561 atggagggca gtatcgctat agtgttttcc attgcgaggt gaagggctct gcaggtacat
     4621 ccgctgactg tgcaggtaag aatggcgttg aagggattat gcaggctgcg gccatcgagt
     4681 gtgcaccatt cggaattcga gtaaacacag ttcatccggg actgatcacc acgcgtgtgg
     4741 cagaaggaca tgttagagtc tgttactatg atacacctga agaaggaaag aaggcaccag
     4801 cagaaagcgc gcccctgaag cgatacggct ccctggagca ggaggtggcc cttatgcttt
     4861 atttagtatc agacgagagc agatattgta cccatggtat aatacgattg atggtggaaa
     4921 agggtttgtg taatgaggag gtaatcatgg atatgtctaa tctaagaagt aaagttgctc
     4981 cagagcgctc tgcactcatc atcattgata tgcagaaaga ttactgtagc gaaggtggaa
     5041 tttttcacag gatgggctat gatataaaat ctgcaaaggc tcttgccgcc aggttgaatg
     5101 attttttggg ccatgcccga aaggttctca agaacataat acacgtcaag atgaccaaga
     5161 ttgattccct tagttcatca acagcgctcg aacagtacag gcgcctgggt gtgaaccgcc
     5221 agtatgatcc agcattttct gaattttatg aggtgttgcc gcaagaggga gaagttgtta
     5281 tacctaaata cagacattct ccctttgtct ccacatattt tgatcagtta ctcaggatac
     5341 gccagataca gacactcata gttacaggcc tggccaccaa tgtttgcgtt gaatcgaccg
     5401 cccgtgacgg gtttgcacgc gattatcacg tggttatccc tgaagattta acagagggga
     5461 caagcctgga ggcaaaaaaa tggtctttat caaatataaa cttattcttc ggtgagattg
     5521 ttcagtccca agatcttctc agatgctggg atctataaga tcaaataccg ggccttgtca
     5581 agaagttgaa gcatacggat gtagcttggc agggaatgtt cggtaaggtg tatgtgtttc
     5641 tgaaggaaat ttggatttga aaacacatac aactcatcgt tgacctgtga ggttttgcaa
     5701 gatataatgg aaaatcagtg tgcagtaaca ggcccaaaaa cgatgtcgaa aatatacatt
     5761 gataaacgtt attttaccga taagaaagcg aaatgggtga gtttcgaaga cacccagggt
     5821 ctcagagaga caaaaaaaga tatttacgga aaatgtgtgc cctgtatcac aaatctttat
     5881 gagcagctga aagatggaaa aactatcgtt gatctcgggt ttgcactcaa gtgctggaag
     5941 gtagtcgttg tactcaacag tatggaggag tgtgtagacc tcctctctga acttgaaata
     6001 tccatgaccg agggtattaa agtgaagggt cgtttcgggt ccgtaaacga gacgaagaca
     6061 accaaggtta ttgtttttaa tgtggctggt gaggctcaaa gaagaaagct ttatgagatt
     6121 ctcaaggatt gtgttaaacg tgtaaatcca cacgctgaga taagttatca tcgtgggtgt
     6181 gtggagcttt accatgaact ttttggagac tggaaaaggt ggaaaaaatt tacccttgtt
     6241 cagaagcctg aggcaattga accaatcatc cgaaagattc gtgaggttct tttctgggat
     6301 aaaccgacat aagcagattt tttcttctcg cagggtctat ttttttgcac atgttgccct
     6361 tgacaatata cgggttcatt atttaagtta atgagttgca gcgtaatggt tgcagcgtaa
     6421 tgataggggc agtcaaacag tttctctggc tctactggta caataccttc cggagtcaga
     6481 aaaccctggg gaatgtgaga agcaagacag agagaaggcc ccggcagcac tgctgttgga
     6541 ggagactgtc tgtctgccga agcctctgag caaattgaca caagtcccag taaattgagg
     6601 atatatgaaa taccgatcag cagcactatt aacactggca catgccgcaa cagacatcaa
     6661 tcagggtgcc atacctgttc ttcttccatt ctttatcgcg caacacaacc tcacctatgc
     6721 cgctgctgca gtaatcgtat tcacaacaac ccttgtttca acgattaccc agccagtgtt
     6781 cggtatcgtt gcagataggc tttcacgtcc gtggctcatg ccctttggcg ttcttctggc
     6841 cggggttggc ttgtcatgtg ttggcctggc accatcctat gaaacaggac ttatagcggt
     6901 tgcactgagt ggaattggag ttgcagcttt ccatccggag ggggcgcgtc ttgcaaaccg
     6961 gtttgcaggc gataagaaag ctaccataat gagtatcttt gctactggtg gtcaactcgg
     7021 atttgcgatt ggtcctctca ttgccagttt tgcacttatc cgatggggga taaaaggcac
     7081 tgcttttttg gcagtccctg ccataggtgt gacgttgttg atcgctttga ttcttccagg
     7141 attatggaga tatcatagcg ttgatggggg gaaggccgta aaaaaaagcc agggggaaga
     7201 tgcttgggct ggttttttgt gtctctccat acttgtcctt tttcgatctg taatcttcta
     7261 cgggattaat acatttcttc ctttattctg gatcaacgtc cttcaacagt cgaagacagc
     7321 aggcgcatct gcactgacag tcttttttgc ttccgcagtt gttgggaatt tgattggtgg
     7381 acgcgtggca gatcacgtgg gctttcgacg ggccattctg acagaatttg cgcttgtcac
     7441 agtacttctt cccatatttg cgtacacctc caatcccttg tgggcaatgt tcctgttgat
     7501 tcctatcgga ctgatgtttt ctgcatcagt cggtcctttg gtggtattag gacaaagcta
     7561 cttgccaaat cgtatcggcc ttgcttcagg agtgacgctc ggtctcgcat tctcgtttgg
     7621 ggggatcaca acacctgttt ttggatggat tggggaccgt cacggacttc atacggcgat
     7681 tgtggtgctg tcatttttac cgataatatg tactggttta actattctac tacccaaacc
     7741 taaagcagca attactgcaa agtaaggtcg tatcccgaat gagccgaact tgttcataac
     7801 tgaatcccgt ggcatctggc gttggtttac aagagacttt ctcggtatca ttcacgagat
     7861 gcgctggagc tatattcctc cgcttatggt ctatctggct gctggaatct ctgggttcac
     7921 aggtataatt gaaagttttt ttgtgaaaga agagttggga ctttcagcag cttttcttgc
     7981 aggtcttggc ttctgggcag gacttccttg ggctttgaaa atgcctttcg gacacctggt
     8041 agatctttac tggcaccata agtcattgtt tgtgtatttg ggagcagcac tcatggcagt
     8101 gagcctcgct atcatgatag ggcttacagg tcacaagcct tttatggcgc agatcatggc
     8161 gcttgacacg tggtatgtca tagccaccct tgtctctcca gtggggtttg tgttacagga
     8221 tgtggtggca gacgctatga ctgttgaggc tgttccgtca tacgatgaaa atggtgtgaa
     8281 gatcccccaa gacgaatttg aaagaatgca tgtaactatg cagactctgg gaagaatagc
     8341 aatcatcggc gggagtgcta tcgtagcggg ggtaggtgga tggcttgcca tgtcaatgtc
     8401 ctactatgcc atgtataagc tatcacttgt cattcctgtt atttcggtgc tgggtgcagt
     8461 aattggagcg tggaatgtac ggaggctgcg ggtccattac cggcgatcag gatgttctga
     8521 aaaagaaatc catgaattgc tccgctcaga aacgtgtgat atcaagccaa gctggtatat
     8581 tctcgggggt ggtatagtct ttgttattgt cagtattatc ctcggtatgt cccagcttgc
     8641 agcaaaaaag gaggtcgttt ttgtatcatc actggcaatc atcatctacc tgatgcatca
     8701 acttctgaag gatcttgatc ctgccaaacg acgggacatc attggtattg caataatcgt
     8761 ttttgttttt cgggcaatgc cgggcattgg cgcgggagcg agctggtggc agattgatgt
     8821 actcaagttt gacgaagcct ttttcggcac tcttcgccaa atatcctcaa ttcttgctat
     8881 catcggtatg tttgcactcc ggggatggat gagccgtcgt cccattcctt accttgttgt
     8941 cttcctttca gcttacgcca ctctgatgat aatgcccttc ctgggtatgt attatggtct
     9001 gcacgagtgg acagagagag tctttggttt tggtgcgaga accattgcca tcatagacac
     9061 tatggctgat tcccctatgg gacaggtttc catgatcccc atgcttgcct ggatagcgcg
     9121 ggaagctccc cttgagcaga aagccactta ttttgctgtc atggcggctt ttactaacct
     9181 tgccctgtca gcatcgagcc tcggtacgaa ttaccttaac gagatttttg ttgttgggcg
     9241 aggtcagtat gaggagcttg gtagacttat gatatgcgcc accatcctcg gcctggttct
     9301 tcctattgtt acagtcattc tggttaactg gcggaatcgc tcaatgtgta agtaagagat
     9361 atttgcatgt tgcagtctgt gtaagtgtcg gcagagtgtc cgtgcgacta ctggatacga
     9421 aaacacacta ctgagcatac tggcatgggg tttgggcatc atgatccacg atgtcttcat
     9481 gtcgttggcc agaaccttga agaaaaaatt tgaacaagaa cagatggtca gcgttgtggt
     9541 tacctcatgt cttgatgtag gttctgaaaa actgacgttc cccctcgtac gtgcctataa
     9601 gaatcaattc tgaccctttt gtaacaggta tgcatggtcc cggattgaca atgaagtttc
     9661 cgtcgctttt gatagcaaca acgctgcagt gagtttcctg gcggattctt gaatctgcaa
     9721 gggtaacacc atcgaggatt tccggtgctt gtacacgaaa tagcgtcaaa ccttcaacta
     9781 acatcaaaac atcctgtttt gtaagaaagc tgaaaaccgc atcgactcct aacacaggat
     9841 aagacatgac aaagtctgct cctgcccggt gaagcgtaga tacatttcgg tccaggttgg
     9901 ccctgctgat aatctggaga tccggcctta gactcctgaa atacttggtg aggtatatgt
     9961 tggttgcatc ctcatgagtt gtgatgagag ctgcaggagc cttttctatc catgccctct
    10021 tgagggtgct taaatcggca gcgtcgccgt atacaatatt ggcactttca ctcgtttttt
    10081 ttgggttttt ttcaatgatc aaaaaaggca catcccgttc agcaaatcgt cttccgattg
    10141 ccgtacccac acgtcctcct cccattatga tgacagggtc cccagcatgc tgaaacatct
    10201 gataaatgga atagacatcg tcatatgatg ctatctgact ctcagtccca gcaagcagca
    10261 ggacagtgct cctggttatc tgtgtctttg gagtagggac tgaaggcttg cctctctccc
    10321 aaataccgac aattgtaacc ccgactgatt cacgcaaatt actttcagcg atagtcttgc
    10381 caacaagggg agtgttgata accggggttt ccgcaataac cagctcgtcg aaacggccta
    10441 tgatatttgc ctgaaagtta ccgcacatgg tccagttggc aaaggaacga gcgaggaggt
    10501 cgggtaattc gacaaccctg gaactacctg ccatggagag aatgtctaca gaatggggtg
    10561 aatctgctgt tgtgataata ggaatactct cattcatttc acggactgta aaggcaatgt
    10621 tggtgtttac ctcgtcgctg tttgtagcaa ccactagagc ggcatcgtga acgcgcattc
    10681 gtttgtaggt atcagggtca tcaatgtttc ctaccgcggc cttgacaccc ctgtcggata
    10741 acaggcctgc atgttccggt ttttcgacta ttaccacgta atcccgtttg tatgtgacca
    10801 gtctttcgac taacgcggct gtcacatcgt cataggctgt catgatcaca tgatctttcg
    10861 tgtctggaga cacctctcgg ggggctcttc ttttagcttc tgattccatc catggtgcga
    10921 ggaaaaactt gatgaacgca aaggggagca ggacaagcat aaagattact ccggatagaa
    10981 gaacgagaat ggaaaagagc ctcccggcat cgctggcgaa cgtaatatct ccaaatccga
    11041 gcgtactcat gactgttaag gtccaataaa agccagtaac ccacgaatac tcccgttctt
    11101 cccatgccat aagaaagtga aagatgatgc tgtacacggt tatcatggct gtgagcatca
    11161 caaggaagcg aatgagcata cgcatgttct gtttggtgct ctttccttcc accaggtagc
    11221 tgactataga agaataaaat ttcacgggct gttgtaagac ctctcaggag aattttttaa
    11281 aagtttatct gcagctaaca tttaatatca actcatttct tctgcactat accataattg
    11341 ctgtaaaaac cttaatctca aagctattat ggattattaa cacaatctgc agtaagaaca
    11401 agaccattta ccttgatgaa tttccattta tgcagcttga aaaattattt cattaccagg
    11461 aggcttaagg ccataacgag cataccagcc actaatccaa aaaggctgtc atggcctttc
    11521 ccataagccc tgcttgtcgg aagtagctca tcgaggctta tataaaccat gattccagcc
    11581 accccaccga aaaggagtcc catgatgtgg gagggaattg ccccgccctc gttgcctaaa
    11641 agaaatcgga gtacaatata gcctattaat gcccccacag gttcagccaa gccactcaat
    11701 agggaatacg tgaatgcttt ttttcggttt ccgctggcat aaaaaatagg aacggacacg
    11761 ctgattcctt cggggatatt atgaagggca actgctacag caatagcaac acccaggctt
    11821 gggtcttcta aggcagcaag gaatgtgaca aaaccttccg gaaaattatg tatggcaatt
    11881 gccagtgctg tgaacaggcc agtccgcaac aacttttgat gcgcaatggc cggatggctt
    11941 gaattttcag ttctctctga acgtgttgct ggctgatcaa acaccggaag gggtgctgta
    12001 ggattacgca ggggcatggt ttcagcctca gtatgaattt cgtgggggtt ttcagtggca
    12061 ggtgtaagat tgtcaatcaa accgatgagc agtattccac cgaaaaagcc ggctgcgttg
    12121 atccagtgtc cccagtattc cccgtattgt tttgagaggg cttcaaagcc catcgggaat
    12181 atttcaacaa atgacagata gagcattacg ccggcggaga atcctgttgc cactgaaaga
    12241 aagcggtagt ctgttctttt ggcagtgaaa gcaataatgc ttccaatccc agtagatata
    12301 ccggcaaaga gggtcagacc gaaagcaatc cagacattct tcatagcatt attgccaaat
    12361 tcccagaggt ttttttatca gtccggacac ccatgatgga gcatgaaaaa atttccctgt
    12421 tttgaatttg gctgtagcaa tggcagaatc catcatgagt tcccaccttt gaagtgttta
    12481 cgtatccact tatttaaact gatatacagt gacgtatttg tctcaatata taaacaagtc
    12541 acttatttgg atatccatgt ttttacttcc cagtcatctt taaacctgtc tttaaagaag
    12601 ccctgcaatt tatccatgag acgtttccag ccggtttcat agtcatattt cagtatgtcc
    12661 tcaaggaaag ggacaacctc accgagttta tcttttggca aataggccct tgctcccatt
    12721 tctatggatt tgttgagcgt ttcaggactt aaggcatgag cggtgagcat ggccaccttg
    12781 agatcttttt ttacagctaa gtcaagaaga tcaaaaccgc ggacacccat aatgtcgagg
    12841 atgacaaggt cgtagggttt ggattccagg agtttgacag cgctttcata ggtgattgcc
    12901 ctatcaaaaa tacagtctgg acaagcatcc ataatctctt cttctaacac actaagaatg
    12961 tccggttcat catcgacggc aagaatcctt tttccattta aaatagaatc actcatttca
    13021 aagcctcctt ttttttaaca tttggtttat tgatgggaca tgtgccttga agagcatctg
    13081 tttcattaac tcagataacc aacgtaaatc gagtcctgca ataatgccgt gccgatatcc
    13141 tgaaaaaatg ttcaaagagt ccacacttaa accggaatag ccattttttt gtatttataa
    13201 gtcttaatag cacaaattaa gatagtgtat gggccagaga cacgggtgtc aacaagtttt
    13261 acaagtttct tagtgaatag aagacaatca cgggtactta agatgatttt gggagaagtt
    13321 ttattgtttc ttatgtgctg acgtatttat tgagatggat ggacagaaaa tcagttttct
    13381 ttgtacacgg ttttttcgga gtttcagatt aactcagaat attccagagc gtggttctgt
    13441 tgaacccatg ctaaaagggc ttccttggct ttttcaaagt caaccttcag cagttttgca
    13501 tggaatcgta cttccgtatt tacaaatgcg tcgaaattgg cccttgatgc aaacatttct
    13561 gggccgttct tataatgggt tatgaagtcg tttataatgg attcaaaagc catgcgttca
    13621 tcgtcacggt tcatgtttat cacctcataa cctccgtatg tgaggatcat gaagaagcag
    13681 gccccaaggg tgctggctta ctgaggggta agctacggta ggaccagcag tgacctcaag
    13741 gcccgtggac tgatctatat ctgttagagt taatcggtct tgaggcagaa accttaaatt
    13801 ataatatttt taaagctaaa aaaacgaaaa gaggctggca cgttctgcat ttgctacaag
    13861 aatcgatatt catttatggt agggcgtttt aacaaaactt aatgattttg cttatgttta
    13921 ttcatttatt ccggttagtg ggttttctat acctgaacga ttgaagtctg atgcgtgata
    13981 ctgcataccg ggctcccaag cccgatactg atcagtcatg agagtactgc tttatgtaga
    14041 tatcaaaaga atgcatcagc ataccccata tgtcatggac agcacataat cagccaattt
    14101 tgccacatga cacattttcc gcagaatagt ttaaattcta agggtggaga ggagagttat
    14161 ataatatgcc tgtttcaggt attcagcgag aaatgtattt ctaaacctgg tacagtctca
    14221 gtccaataat acccaggaca attaaaataa ccgataagat gcgaggggta tttgcaggat
    14281 cttcgaaaaa ggctatacct atcaaaaaag tgcctgcagc cccaatccct gtccacacag
    14341 cgtaagccgt tcctatggga atgctcttct gtgccaccca aagaaacagt ccgctcagcg
    14401 tcattgaaac tatggacaca agtatccagg taaccttgtt tgccgtggtc tgcgacagtt
    14461 ttaatcccag tggccaacca atttcaaaaa caccagcgat caacaggaac atccaagaca
    14521 ttgtgtttac ctccgtatta tcgattataa ccagggttcc tgttgttcat tcgaggttca
    14581 agatgctgtg cagcatctgt ttgggaatgc tcagttttag tgcccgggga aaactgatgg
    14641 gacccatcca ctatgacccc ggctctcatc ttccttgaat ctctcctgaa attcaacaac
    14701 ctctgtgaaa aatgcaatgg gaaaggcagg ataacaaggg gctcctgttt attatccagt
    14761 tataccaacg agtatcctct ttctttgaca attgattcac cacgtttcac cgactgtgtt
    14821 atggcagcag gggaaagtct gagccggcga gacagttcgg cctgatttgt tcccaattct
    14881 cggacagtcc aataacagaa aaggctcctt gcattcaccc tgctttgtat tttaccaggc
    14941 gtgaaaatgt cgcatctgtt acaatgcagc acctcacaca cgcgactgat cacagcagta
    15001 aggtcaattc cgcgctcttt caaactgtat ctccagtcag aattctcatt cgctgttgcc
    15061 aatgcatggc agacgaactc atgatctccc aacattcgtt catctgattt ctgatactcg
    15121 ttggcacgtc gttttgcaat tacttgtttc cacccgccgc tactgcgaat aagacctccg
    15181 ccagtgagat cgtttcggtg ccccattggt aaccctttgg caatgaattc atggtacttt
    15241 gctctcgcag catcaaggtc ggttgcgaac aggttaagca cctcgcttgt gttctgccag
    15301 ttgttctgcc tttttcccgt tagcacgcca tgaccgcaat atgcgaagcg agccagttca
    15361 ttcatgttcg ttgcaatacc ggttcggatg gggttcaaat ggatgtacct caccagctcg
    15421 agaaagtacg gttcctgctg gcacaggata gacttatatc tgttctggaa gaggtggccc
    15481 gaacgaatgt gacgcctgtt ataggtgact gcatatcctg taaggagttt ctgcatcatg
    15541 cgacctacag gtgttgaacc ggttctcaac aggagatgga aatgattcgg cataagcgtc
    15601 catgcataac actgggtggc tgtttcattt aggacagttg cgagtcgctg tacaaagcta
    15661 tcatagtctg tgtggtcttg gaagatgatg ccctgctcga ttcctctgac gattatgtga
    15721 tgcaaggcgc cgggagcgtc tatacgcgcc tttcgtggca tagtgagagc ttaccacgga
    15781 tacctataaa aagaaactga aaatgtaaag ggtgtcccca aagtcttgac attgatggtg
    15841 aatagctggt ataatcttac caatcctaat tctactgata tgaaagagga gtaaaaacat
    15901 ggaagagatt gcaaaaattt cgattgatga atacgagagg agaccggatg gttcgtgggt
    15961 ttgcatcaag aactcagata tcacaacgaa gtctaactgg gtaatcaggg tatccccagg
    16021 tgtaattttt caaaaaaatc gcaggctctt tggactaaat gttgcagatg cgctcgataa
    16081 gattagtggg aattaaggaa gcagaagagg caatccggcg atctcagcaa gcctaccaga
    16141 agtatagcgt tcatggctcc tgtgcctgta tcatacaatg gccttttttc agttgacttt
    16201 atcgagagct tctttgggtc agtcaaaagt taaggaaaca caagcatttt tctctggctt
    16261 actccagtaa atagttacag gaattacagc ctatcatata tgaatccggg caacaactta
    16321 atcaaccaag ctaccattaa ccatcgccgt gtgatataaa cactgcgttt ttttgatttt
    16381 atggcgtggt atatctgcct tgcagctttt cccggctcag ctgcccagaa gatgccatcc
    16441 cctttggcca ttgcggtatt aacaaatcct ggttttatat cagttattgt gattggcagc
    16501 cctgattttt tgaccttctg ccgtagcccg tccagataat tggactcaaa agcctttgat
    16561 gcattatatg ccggagactc tctgcctcca cgtaacgctg ctaccgacga gattcctact
    16621 agatgccccg aacccttctc tgtgaaatga tgcatgataa cattaattaa agcggcaaac
    16681 ccattgacgt ttgttttaat ggtttcgttt tcaatatgcc agcttaggct atcgtttata
    16741 tctcctgtac cggcactaat cacaataagg tccacacccc ccatttcttt taagaactca
    16801 tttaaagtct ccattgccac ttccacgttt gcaacatcaa ttttttgaac gaataatatc
    16861 ccttccacgt ctttgcgcaa ctcttccaat agctggactc gccttgccat gataccaacg
    16921 atgtacccat tctcagacag aattttggcc aattcccgcc caatcccgga actgcctcct
    16981 ataacgattg cttttttcat gattgttttt ttattgtaat gacagagtca ataacgagtg
    17041 taatgaaata tttactcccc ataccactgt ctgctgcgga tattctgaag ctgccagggg
    17101 ctttgaatat cgagcctttc agggaagtac ttgccgcggc catcaagggg ggtccactct
    17161 gtgaattcac caaccatgcg gcccagatat ggggttgccg tttcaagaca ttcctcgaaa
    17221 tccaagtcct caggttctac cacgccccga cgaggatggc gtacggccca aagaacagct
    17281 gcaagtgcac ctgccataac ctgcacggta gtcgagtttg caaatggcac gtgcctgcgt
    17341 gcctcctgga tcaagagctg cgaaccgtac cagtatgcgt ttctctcatg acctgccagc
    17401 aggacaccga gctcatccat cccctcaata atatctgttc caaggcgtcg gcaggagggt
    17461 tgcagaatcc atcccctacc ttccagttcc agtgcagaca acattgcatc gtcgcagggg
    17521 tggtacacaa acataacgct cggccgatat aggaacttac tgccatcgcg gatcgacagg
    17581 tagtcggcga tcgagaaaac ctcatcatgg gtcacgagca tcccctggaa acccccgatt
    17641 gagggtgccc agctacgcgc aaaggtgctt gccccgggac gttcaaggta cagtgtaccg
    17701 gaaccgcggc gatgctcccg agcccccttt ggcttacgtt tttcaaagac gccgaaggaa
    17761 agttcggctg gctgcatcaa ttcatccaca aaaccgtcta tagaccaggt attaatgaat
    17821 tcaccgtgcc gtttcggtcg ctggctggcc tgggtgtcgc gctctgatat ttgaattgtg
    17881 gatattccga gatcatgcgc aagattcgcc cactcttcgc gcgaggatgg ttttgtattg
    17941 ccattacgaa ctatttggtc cagatcaaca agggcgcgtt tgacaaagtg agacacaagt
    18001 cccgggttag cgccgtgatc gacaacggca gtaggtgaat caggtcctaa ctctgcagcg
    18061 aggtctatga tcttggcgcg tatggcgtag ttcgtgcgct ggtgaagttc cagcattggg
    18121 ttgtcgaata cacccggcca tggttcaatt gaggtatcta cgtacagagc accccggacc
    18181 gcgcaaaacc ggatcagatc aatactggaa acgtcaattg aaaggttcaa catgagatcc
    18241 ccgtcactga cgtagttttt tagtattgag cggtagttgc ctggtgtcag gttgcagtgg
    18301 atgaactttg ctccacagcg ctcggcaatt cttcgtcctg attcgtcagc tgatagaacg
    18361 atcaaccggt catgaattcc ctcaaggtgt cggccaataa ctggaagaac ggcctgtcca
    18421 atgcttccgc atccaatcat tatgacacgg ccctcgaaaa atggagtcgt cagatgttta
    18481 ttcattggtg tcaccatcct ttactccagt atgttcttac attatgtcag atttgtccag
    18541 cacaaaaatc agagacgggt gtaacgcacc cgcccggttg tgctttcatg tactctcttt
    18601 cgtcacatgt caacatttca gggatatcca caaagtaaca ggtaatcaat aaaaaaagaa
    18661 tattcttact tgcagacact ccgtttacgc ataatttcca aatcttttcg aagctgttct
    18721 ctctcttttg tacccgccaa gttccagatc cagaacccct tgaggatcat cgccagacct
    18781 tttatccccg aagttttcta tatggtcttt ttcgtgttca aggacagcat tgaacaggga
    18841 gattccggct aacctatgac cgcagtcagc aataaatcca ttcattgccc cattatagcc
    18901 gggcagattg gtagacctgt cagtattgac cacgatgaca ttttcgtatt tacattgtaa
    18961 gagagttatc gtgggccagc atatgtgttt tcacgtcctt ttttggcttt cctgtcttgt
    19021 ctacggcaca gtcttttctg tgtcataaat gagagagaga agggtactgt tcaccacccc
    19081 ttcgctggaa aggccttgag ttacttcagg tcagagcatg acaggaatga attgtcacgc
    19141 aacctgctga tgtatctttt gttcgtgctc aatgatccct tgaaggatcc ctaaacaaca
    19201 cccatttaga ttacttacgc caataattat tggcagcggc gattgtctga aagttgatgg
    19261 caacaacctg tgacgccgct atgagcgaat ctgcgttgtt ggagtacacg ggacgaagtt
    19321 tttggagaac ctccgcgttc ggttgcgtgt acttgactcc ccgcctgctc agagtgattg
    19381 caagctcaaa cccagcctgt ggtgtatcag tgataagtgt ggtaagccat gtgtccgcca
    19441 ttgtttgacc ctccttgtga taaaattgtg taatctcatg attacattat ccagcaatta
    19501 tcatagtata caatgcaata ttcatatttt ccgaattata ctctgaagat tttatattga
    19561 caacagaaaa gcgcaataag ttatcatgtt gtttctgtaa tgtgttcgtc ataaacagga
    19621 agatcaacgt tgccaattag aataactgaa caacatgcct accagtccta tttgccggga
    19681 gaccacaagg ggcactgttc atgaatacat tctttgttct gcaggaattg gtcacacgcg
    19741 aaagtatcca ccttcaatga tttcccagtc tgtttctgta tgtgagctgt aatctctttc
    19801 aatgtcagcc atgaatctct ctggcagcaa cgtggagctt tgtaggctgc tattcgtgca
    19861 agcactgcct gtgttacctg aagaacagcc tgtctcttct ccccatcata tggactggcc
    19921 ttcagtataa tggatatcgc aatgcctgcg ccgattgcag cgccacatgc gccgagaaat
    19981 gcacatgcac cgccactcac agttttgccc cgttcgatcg ctgtctgtat ctcttcattt
    20041 ataacagggt agccgctgtt tctaagggcg gtcaggataa cagccgggac catggagtga
    20101 tgttcaggtc cgtgcatgcg gaataaatgg tgagacctga tagtctgcat aagttctgcc
    20161 atgtcagtct gtctgctgtg caaacagaca gtttttatta tttctatggc atctgcacta
    20221 tggcagtgat cgcacacaaa atgaccgtag ggacatgctg catttgcctg caaaacctgg
    20281 ccacaaaaaa agcatgtgct ttctctgttt ttccagaaat agatcagggg taacccgcag
    20341 actgcacacc cagatttgta tatatcgatg tgctgaaggg aatctctacc tgtaatttcc
    20401 attcccgggg aacagcatgt tttttcttca tccggagaac aacagggttg ttctccgtca
    20461 atgttttcag gaattactcc agcgttggag ggcctcattt cttctgaatg atcttttttc
    20521 atatttttgc cgcgaatccc atatttaagt cagcattttg tcaaatacct tacgcaaaag
    20581 ggaattgtct ttccatcctt atatacatga aggctgcatc tgcgcagccg ttcaacgtcg
    20641 agagtatatg catcctgaaa agccatggct gttatagtaa agccatgtgt cctgacacgg
    20701 tcaagaaaag catcaaggta gataaacccg tcattgtcaa agtattgctc tgtgctttgt
    20761 gtggcagcgc gcctccatct tctggataca aagtttctgc tcttaagatg ctctgtgccg
    20821 tcgggctggc agcaggcatt ggtttgattt tttggtgtaa gcttcatcag gccttcgggt
    20881 agaaccacaa aatccccatg aaatccgcat ctaggatgat cacaggcaga aggcagaaaa
    20941 tctgagatat tgactctgcc catggtctgc ctctcaatgg cacacagtac ctcaggaagt
    21001 gtgattctgt ccttattttc cggtggtcgt ggataacggc cgaaataact gacaggctga
    21061 aaatggaccc ctctcactgc cggagagtgc tcaaatccga atctcagtat atctccgata
    21121 ttgtgatcat taactccagg tacaagagtg ggcgcaagcg ttacgccgat catttcatcg
    21181 gaacaggatt cgatagctgc ctgtttttcg gcaaacagag gccttccccg caacttttca
    21241 taaatgacgt cttcagtacc gtcaaactgc ataaaaacaa aggaaaggcc tgcttgcgca
    21301 agtgccctag taaacccttt gtcctgccca agcctgatac catttgagtt cagctgtatg
    21361 ttttcacagc ctgctgcctt ggctgcagct actatctcag gcaggtcatt cctgactgtc
    21421 ggttcaccgc ctgatagctg aataaaggta ttcccgtttc tcacaagttt ttcaaaattg
    21481 tccatgattt cagaaattga tgggtcctgt gttgttccgc cgctttgcgc aaagcaaact
    21541 gggcatttca ggttgcatcg tgttgtaacc tctatcagta cacagcaggt tttttgtaaa
    21601 tgaccagggc acaatccgca ggaatccggg caatcagggg gcctgtcctc atttacggga
    21661 gcttgatagt taccccatcc ctccattgga ataacatccc ccctccagat tatcgctgaa
    21721 aagctgccat gatcagtaca ggatttctcg ataaaaaaat cattaccctg tttggttatg
    21781 gatgcaggta ttctttttaa acatttcgga catacactgt atgtattttg agttataata
    21841 gccacattaa tctcttagcc ttatttccct cttcatgttc gtaagtccgg ggattgactg
    21901 tatgtatcaa actgcaccat acaggatatt atacccagat ttgtcgtaat atccaaatat
    21961 atgaataagt actaacagcg ataaaaatct cagtaaagcc tttcgtctca gtaagcagcc
    22021 accagtttag tatacttgtt ttttcaacca gggcaaacag cactcatgaa tggtgtcgca
    22081 tgcatctttc tcctgtgtta tccttatcgc cggtatctgc gtgatgagca catccaccac
    22141 ttgtagagca ttttcagacg ccttcaacga aaatgtctga gtggcaaatg gtccggtcaa
    22201 tgccattgtg accgagggca acactgcctt cataggcggt gaaattaccc gtgttgactc
    22261 caacacaggc catgatgttc tcctccatac cacccagaac ctcatgccgc ctttccaaag
    22321 gtgaatagtt ttgtcttcac agcagtactg attgtttcga gggcaggtgg gggtgtggta
    22381 tctatgggta attttgcaca gatgggcagt attaccagga acaatattgc ccgtattatt
    22441 gctccctgag aaagggcaaa agtaagaact attctggaaa gcaccaaaag ccgccaaaaa
    22501 aagtggtttt ttgttgtgcc atttcagagc agaaccaatc caaagcctgg ttttgatcag
    22561 ttatgtttta tgtcagaaac cgtgtataat atccgtaccg gcgttcctct atggaggttt
    22621 ttcctgtacc gtacctggca gcgcatattt tgagtacccg ggattgatcc tcattgtcgc
    22681 tgcagattga cacatctttt caacggaggt gcgtcgggga tgtccggtgg agcaggatgc
    22741 caagtttgtt caccagaata atcacccatg tggacagcac aaagggagct gtcaaggcag
    22801 ggatactg
//
LOCUS       DAOOMC010000032        22484 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdus sp. isolate AMR_MDS_2685
            NODE_2225_length_22484_cov_5.940211, whole genome shotgun sequence.
ACCESSION   DAOOMC010000032 DAOOMC010000000
VERSION     DAOOMC010000032.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864760
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdus sp. (wastewater metagenome)
  ORGANISM  Syntrophorhabdus sp.
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae; Syntrophorhabdus.
REFERENCE   1  (bases 1 to 22484)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22484)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,729
            Genes (coding)                    :: 2,708
            CDSs (with protein)               :: 2,708
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 7 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22484
                     /organism="Syntrophorhabdus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2225_length_22484_cov_5.940211"
                     /isolate="AMR_MDS_2685"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2699756"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(424..1041)
                     /locus_tag="PK125_04810"
     CDS             complement(424..1041)
                     /locus_tag="PK125_04810"
                     /inference="COORDINATES: protein motif:HMM:NF012271.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HPB37463.1"
                     /translation="MHHSKANNKPIKRSTTQTIFFIILIFSAITASAHYAHGTSVTLA
                     WDPNSPAENVVGYRLYYGNESRNYGFMVEIVGETLKKISGLKKGQNYYFAVTALNGAG
                     QESDFSDEVAITTCTYNLFKKKRVFNATGGVKKIRVRTQPQCEWSVQSDQSWLRIMEG
                     DEVHAGKGFITYSIDPNPDPEKRIAYLMIGEKRFKVIQKGSTLYE"
     regulatory      complement(1104..1192)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            complement(1403..3316)
                     /locus_tag="PK125_04815"
     CDS             complement(1403..3316)
                     /locus_tag="PK125_04815"
                     /inference="COORDINATES: protein motif:HMM:NF039835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB37464.1"
                     /translation="MITRPLLVLILALSSLSIFLTPNIGSAGQVTLAWDMNNEPNVAG
                     YKIYYGKASRNYDWFIDVGNITQVTITDLPNGSTYYFAATAYDNSNPPIESTYSDEVN
                     KDMCTYSISQTNASFSASGGTGSVSVTTQPGCTWTASSGASWLTITSGKNGTGNGVIC
                     YSVSANTNTDARTASSTFAGKVFTVAQSGATTYKITASASQGGIITPSGSVTVAQGAN
                     QSFTVTPNAGYQVSYVAIDGVPQEATSSYTFSNVTKDHTIAAVFSVKTYTINASAGPG
                     GSISPSGNKSVNHGANQSYVISPNNGFTIADVIVDGVSQGATGSYIFSNVTSNHTISA
                     VFKVNSFTITASAGPGGTISPSESISVPNGGSQTFTISPDASHAISDVLVDGSSVGAV
                     SSYTFRNVTGDHTIYALFVPGYTLSITTSGTGSGHVVAHPLATAYAPGTKITLKAIAG
                     EGSNFSGFSGDCESNRSKCTLIMNQHAEINAAFEQKNFKLIATAIGNGSISVEGLASI
                     KNKKIKNKTKIKRSKQAITVSYGSQLVYHINPEPEHYIKKIIVNGKPIEAVDTLTFAN
                     IKRNHRVRVRFESQAKFIQNPFKKTIAKQVVLQDIQDRDISEAQQFDSYDTGEDTSIP
                     PPSYTDQYASLQQ"
     regulatory      complement(3382..3472)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            3748..3842
                     /locus_tag="PK125_04820"
     tRNA            3748..3842
                     /locus_tag="PK125_04820"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3782..3784,aa:Sec,seq:tca)
     gene            complement(4081..5472)
                     /locus_tag="PK125_04825"
     CDS             complement(4081..5472)
                     /locus_tag="PK125_04825"
                     /inference="COORDINATES: protein motif:HMM:TIGR00711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPB37465.1"
                     /translation="MRKYLVFFIAGVSILMYAIDATVISVAFPNFTKELHASVLLSAW
                     TISIYFIAVTMTMPLAGNLSDSFGRKKVFLFSLLVFTGSSLACGFASSIYALIAFRFF
                     QGIGGACFLPTASGIASDFFPESRETAIGLLSSIFGVGFVVGPNLGGWIVSQYSWRYV
                     FYINVPIGAALICLTMMLLKKSKNITRPRVDFIGIGLMSSSLLLLLLGLNLVGERPSA
                     VSMILAGILVFISFFLGICFLKQEKRAHAPIIDTKLLESRAFVAANVSNLIVGMTSMG
                     LFSFIPLYAISVHKFSTLMSGMILTPRSLAMTVTSAATSFLLRRLGYRWPMVIGFITM
                     GLGIILLTPGLLLTSAGGALGGVIGTFSIIMFLNGISAGMVFPSANNACIELMPEKVG
                     TIVGLRNMFRNIGGALAISLITFVLHLSPDAFTGFMIVFISFGIMPLVAVPLLFLMPA
                     GARSPRSRIPRQL"
     gene            5670..6260
                     /locus_tag="PK125_04830"
     CDS             5670..6260
                     /locus_tag="PK125_04830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB37466.1"
                     /translation="MKRVGMAVLAMAIFFGFSGIVCAQEKSSDVIRRSYSVYSTNLFQ
                     GKQLLKQGDFLWARAHFAKALESQKWPEAVAFAATASYKMNDMRAAEHYIADAEKMTG
                     KGVFELRIAGYKALIYLKEGRQDEGLKALREYVALYNYLDPLMSIREVKAMAIKGMVN
                     LSMLEALLDEQIIWREDEIEQYEKTGTGFYGRYGGP"
     gene            complement(6513..7802)
                     /locus_tag="PK125_04835"
     CDS             complement(6513..7802)
                     /locus_tag="PK125_04835"
                     /inference="COORDINATES: protein motif:HMM:NF024873.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H-like domain-containing protein"
                     /protein_id="HPB37467.1"
                     /translation="MSLRDRLGRLTGEPAIPNQADSPQKAIGELRRKIDEIMSRRERI
                     ARPRFAEAWQKAIPLEHVVAGEEIRTQHGNLFVSRNSLKGNDSHGHVHISDFACLNME
                     ATAFLSYPPMTIDLALEDGLFLDTETTGLAGGTGTFPFLIGLGWFDAGSFITCQLFAR
                     DFSEEKAMLRYLSDIASDKQFLVTFNGRAYDLNLLATRFILNRFQDTLTVKPHIDLLH
                     PSRRVFAHRLENVRLSTIETNILGVWRNGDVPGFEIPQRYFDWLRVRDGKLLEDVFTH
                     NRMDIISMASLLKHLADLTETCQEAGQGQHIDFLKMAILLYERGNPEKAQKMLESLLE
                     SNRIDVAIGARRSLSLMYKKARRWKDAIALWQEIVAINPHDVFAIEELAKFYEHHTRN
                     FGKALEMVRKLLNEARNLSDTERESLEHRLHRLHRHK"
     gene            complement(7799..10159)
                     /locus_tag="PK125_04840"
     CDS             complement(7799..10159)
                     /locus_tag="PK125_04840"
                     /inference="COORDINATES: protein motif:HMM:NF012492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HPB37468.1"
                     /translation="MPHISLTEWLTRAEKDRRFRENATAIRHIPALQGSYAPYPHWVN
                     PLLQKILTGQGIGKLYSHQAQAIELVHQGRDVVLVTPTASGKTLCYNIPVLQKILEEP
                     ETRAIYLFPTKALANDQMTEIHGLIRELKANIKTFTYDGDTPDDARQAIRKQGNVVVT
                     NPDMLHAGILPHHTKWQKLFSNLKYVVVDELHIYRGVFGSHVTNVFRRLKRICRFYGQ
                     DPVFICCSATVANPREHAEDLLEKEVVLIDQSGAPRSAKTFILYNPPIVNRELGIRQS
                     ALTPARTIASNLIDNRIQTIAFTTSRLNVEVLTKYLKDTFKSKIPKDDHYVTGYRGGY
                     LPNLRRQIEKGLRERSIMGVVSTNALELGIDIGDLEACIMVGYPGTIASAWQQAGRAG
                     RRTGSSLAILIAKSNPMDQFIVENPDYFFSRSPEHCRVNPDNLLILLHHIKSAAFELP
                     FEEGEKFGKEDLVELLTYLEEKGVIHRVDQRWHWSAESYPADEVSLRTVNPENIVVID
                     TTDTGNHKVIAEVDWDSAFTAVHDSAIYMVESQQYHVDKLDLERKKAYVKKVDVDYYT
                     DAMTYTGVRVMDDFDTKMKGRLIVQHGEVQVTRKVVGYKKIKFYTSENVGYGDVDLPE
                     REMHTTSYWFTIPWDLLYSLQLSRLDILDGLAGLTYCLHHIAAMLVMSDIRDVDWCIG
                     DKSGEWYVRRGDNRRMITTSPSKPVNVMENAYDPTVFIFDAYPGGIGFSENLFERHNE
                     LVRAVRSVITSCPCEHGCPMCVGPLMEVGPSAKSAALKILDLMDTP"
     gene            complement(10147..10428)
                     /locus_tag="PK125_04845"
     CDS             complement(10147..10428)
                     /locus_tag="PK125_04845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB37469.1"
                     /translation="MISYVRQVAICESVRETIRQALSRSDDPGVRQKTRDIPPCDSIL
                     RTVSLNQNLDTEEKLIDFITEHAIDSLRLTPEQKEQLTLQGDEAGTCPT"
     gene            10725..10931
                     /locus_tag="PK125_04850"
     CDS             10725..10931
                     /locus_tag="PK125_04850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015863587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HPB37470.1"
                     /translation="MAKGKVKWFNESKGFGFIAQEDGKDVFVHFTAIQGEGFKTLSEG
                     DEVEFDVEKGQKGPNAVNVKKVKA"
     gene            complement(11046..13148)
                     /locus_tag="PK125_04855"
     CDS             complement(11046..13148)
                     /locus_tag="PK125_04855"
                     /inference="COORDINATES: protein motif:HMM:NF012602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HPB37471.1"
                     /translation="MNDVEILKSACVLCQAGCGILVHIQNGEIVRVEGDPESPFNHGR
                     LCLKGQTALEHLYHPARLKYPLRRSGERGNGRWDRISWDEALNEIAEALKKTKETYGP
                     ESVVFIRGSFRGGYEGGYIGRFANVFGTPNIASMAPVCFIPRVNGSLLTNGFNPVPDY
                     GYPPSCMMVWGANLSETRLGEGMDTLKALQRGSKLIVVDPRKFDLSRKADIWLQVRPG
                     SDLALALGLIHVIITEGLYDRVFVGKWTVGFDQLKEHIAQYTPEIVEKVTWIPANLIR
                     EAARLYATAGPAIIQAGNAIDHTGNNFQTARALAILRAITGNIGVPGGELACSQPAIV
                     PMGSPEFDLRDKLLPDVRSRRLNASDGFLPSVFYALPQSIVKAILKGEPYPLHSALIF
                     ECNMLQTYSNARHVYKALQKIGFSATIDMFMTPTAAMSDIVLPVGSFLEINNIIAPPY
                     YPLAQVQQKVAQVGECRSDSEIITALARKLGIGEYFWETEKECLDFILKPAGITFDEF
                     KKIGTLVGKKEYRRHEKDGFPTPSGKVELYSRRLKDWGFDPIPIYREIPDTPLSSPEI
                     AREYPLVLTSWKVEEFRHSSQRQIESLRKRHHEPEVWIHPDTATALGITQGDTVYIET
                     KQGRITQKARITEDIDSRVVGVDYGWWFPERGPEVMYGWEEANVNILTDDSQPWGAEM
                     GSPNLRGIACRVYKAHQK"
     gene            13474..13938
                     /locus_tag="PK125_04860"
     CDS             13474..13938
                     /locus_tag="PK125_04860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB37472.1"
                     /translation="MKKTTLLFAAIILFSTVLKLHGAEWISIGKDRLGNELFYDHEVI
                     IERSKDIMEVQMKGIYSDAGRRERIQDRTRAKSTVERYETLSYSLELQQINCVKREFR
                     VMAYTDYSSDGGVLYKFIAEQQAMKGWEPIAPDSMGERMYKAVCRPSSGKKN"
     gene            complement(14037..15368)
                     /locus_tag="PK125_04865"
     CDS             complement(14037..15368)
                     /locus_tag="PK125_04865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB37473.1"
                     /translation="MARPDMISLWRTGISEKRYRANAVFQICVRFSFLFFTAGLVVLL
                     FAVPIMAAQVTLAWDSNTESDLAGYRIYYGNASRNYTMFIDVGKVNTYTVTGLTDGNI
                     YYFAATAYSTSNVESAYSVEVNHRTCSYSISPPSASVTQQGSTGTVQVTTQAGCPWVA
                     SSGAPWVTITSGNQGTGNGTASYSVSANTGATARTATSTIAGKLFTINQAGTGSTYYN
                     ITASAGTGGTISPPGTVNKSAGTSQTFFITPGPGYAISSVVVDGTPVGAITSYTFHNI
                     AANHTIAASFVSSVPVSSSTYTITASAETGGTISPSGAVAVSRGASKSFTISPSQNYD
                     IYTVYVDGSWKGPISSYTFTGISANHTINAKFTLKTFTITASAGKGGTISPSGSVSVP
                     YGYGKYFSIRPNTGYRVSRVLVNGVSKGAVTYYRFDNVTKNHRISAEFVKK"
     gene            complement(16036..16545)
                     /locus_tag="PK125_04870"
     CDS             complement(16036..16545)
                     /locus_tag="PK125_04870"
                     /inference="COORDINATES: protein motif:HMM:NF017774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HPB37474.1"
                     /translation="MLHLIIDGYNYIHRIRSGPIDRSSNLDMLRRELLEKLFRYKRDR
                     SVRITVVFDARGGYNLGRQRESYKGINVVYAGEGETADDVIIGWIRERRSGQIIATSD
                     RAIIDEAKKAGVPFMTPVAMEQSMKSGKEELDEKDDDSGFRRPKTGNPRKLPKKLRKA
                     TKSINKIRF"
     gene            complement(16593..17015)
                     /gene="nusB"
                     /locus_tag="PK125_04875"
     CDS             complement(16593..17015)
                     /gene="nusB"
                     /locus_tag="PK125_04875"
                     /inference="COORDINATES: protein motif:HMM:TIGR01951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HPB37475.1"
                     /translation="MRRRKARELALRMLYQVETAGEDPELALVRYCESFPYQQDILDY
                     TRLLLSGVRQEQAAIDKSIENASEHWKLARTTYVDRSILRLAVFEMLYTPDVPPKVAI
                     DEAIELGKKYGNEESRDFINGILDRILRESYKKSAVSD"
     gene            complement(16988..17476)
                     /gene="ribE"
                     /locus_tag="PK125_04880"
     CDS             complement(16988..17476)
                     /gene="ribE"
                     /locus_tag="PK125_04880"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: protein motif:HMM:NF000812.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HPB37476.1"
                     /translation="MIHEGKLSAKGFKFAIVVSRFNSFISDRLVDGALDALKRHGAED
                     KQVDIYKVPGAYEIPLVAKLVAKKKDVDAVICLGAVIKGATPHFHYVASEVAKGIALA
                     SLDHEKPITFGVITSENVEQAIERAGTKSGNKGYDAAMAAIEMANLLKDKALCGDGKQ
                     ES"
     gene            complement(17486..18691)
                     /locus_tag="PK125_04885"
     CDS             complement(17486..18691)
                     /locus_tag="PK125_04885"
                     /EC_number="3.5.4.25"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: protein motif:HMM:NF006803.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA];
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HPB37477.1"
                     /translation="MAISKIEDIISDIAEGKMVIMTDDEDRENEGDVVIAAEKVTPEA
                     ITFMARYACGLICLSLTDEKAKALNLPLMVRENTSKHNTAFTISIEAKKGVTTGISAH
                     DRARTVQVAIDDNSTEDDLARPGHIFPLIARKGGVLRRVGHTEASVDLARLAGLKPAG
                     VICEVMNEDGTMARLPQLEVFAKQHGLKIGTVADLIQYRMANEHLVTRAAETKIPTRY
                     GGEFNLIAYENDMDRQEHLALVKGDIKPDDVVLVRVHSECFTGDVLGSMRCDCGEQMH
                     AALKMIEKEGKGVFLYMRQEGRGIGLVNKLKAYCLQDQGHDTVEANIALGFAPDLRDY
                     GVGAQILRDLGVRKMKLLTNNPRKIRGLEGYGLTVVERVSLEMEPTKENITYLKTKQE
                     KLGHILNNI"
     gene            complement(18694..19341)
                     /locus_tag="PK125_04890"
     CDS             complement(18694..19341)
                     /locus_tag="PK125_04890"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HPB37478.1"
                     /translation="MFTGIIEDIGSILSIKKSSGKWEFFIKTALAKAGIREGDSVAID
                     GVCLTATAITGDGFVADASLETLKVTTLAEKKTGTRVNIERAMGADGRFGGHFVMGHV
                     DCIGTIVDIRKAGDSQRFSVEIPLDFARYIVKKGSIAIDGISLTVNEQRHNIFTVNII
                     PFTTSKTTIGEKNPGDKVNIETDIIGKYIESFLIKDKNKGVDLDFLYRHGYVKGE"
     gene            complement(19322..20425)
                     /gene="ribD"
                     /locus_tag="PK125_04895"
     CDS             complement(19322..20425)
                     /gene="ribD"
                     /locus_tag="PK125_04895"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: protein motif:HMM:TIGR00326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HPB37479.1"
                     /translation="MKDEQCMHLALSLARKGMGMTSPNPMVGALLVRGNRIVGKGYHR
                     KAGTPHAEIVALNEAKEEAKGATLYVNLEPCAHAGRTPPCVNSIIAAGVRRVVVAMLD
                     PNPLVNGEGVNELKKAGIEVKVGLLEEEAKRLNEAFAVYMEKKRPFFTMKGAVSLDGK
                     IATKTCDSKWISNEESRRFVNKVRATVDGIMVGINTVILDNPLLVPKIARPRKYPVRI
                     VLDSKLRIPLSCDLVKTSEKYKTWIFTSEDSRPDKEVKLRAMGLDVIRVSKDESGRVS
                     PKHVCEVLYQREIMHVLVEGGGEINSCLLKEGFLDKIMLFYAPILIGGKGAYNLIGGK
                     GIDFLKDAYKINIAAVKRFKEDIYVEGYVHRNY"
     gene            complement(20528..20995)
                     /gene="nrdR"
                     /locus_tag="PK125_04900"
     CDS             complement(20528..20995)
                     /gene="nrdR"
                     /locus_tag="PK125_04900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003762439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HPB37480.1"
                     /translation="MKCPFCGFLESKVLDSRISKEMDTIRRRRECLKCAKRFTTAERL
                     EEGLPLVIKKDDRREVFDRLKILNGLKKACEKRPISITHLEKIVSRIEYTLLDRGERE
                     IKASDIGSMVMEELKRLDDIAYVRFASVYRQFRDINELMDELKGLLLKKDESP"
     gene            complement(20992..21573)
                     /locus_tag="PK125_04905"
     CDS             complement(20992..21573)
                     /locus_tag="PK125_04905"
                     /inference="COORDINATES: protein motif:HMM:NF025793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dCMP deaminase family protein"
                     /protein_id="HPB37481.1"
                     /translation="MKINRPDWDSYFMDIARIVSRRSTCIRRNVGALIVKDKRILSTG
                     YNGAPINLKHCIDTGCMRERLKIASGERHELCRGLHAEQNAIIQAAYHGVSINGADIY
                     STHLPCSICMKMIINAGIAKVLYADGYPDELSHEFILESAIVVQKIDFEKKSALDKTV
                     QGSEFPAWPGQVSVRSVNQHVKVKNFKLRSKKA"
     gene            complement(21570..>22484)
                     /gene="glyA"
                     /locus_tag="PK125_04910"
     CDS             complement(21570..>22484)
                     /gene="glyA"
                     /locus_tag="PK125_04910"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: protein motif:HMM:NF000586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HPB37482.1"
                     /translation="LGMDIAHGGHLTHGARVSFSGKFYQAVGYHVRKDNEMLDYDEIR
                     RIALAEKPKMIIAGASAYPRLIDFKKFREIADEVGAYLTVDMAHIAGLVAARFHPDPV
                     EYSHFVTTTTHKTLRGPRGGMILCKKEFARAIDSAVFPGIQGGPLMHVIAAKAVALGQ
                     AMTDSFKDYQQQIIKNAQRLCSALEKKGYRIVSGGTDNHLFMVDLTDKGLTGKEAQES
                     LEASGIMINKNLIPFDTKSPNVTSGIRVGTPAVTTRGMKEGEMDLIADLIDAVLQRPA
                     DEALQSATRERVKGLCKSFPFYSRIYDL"
BASE COUNT         5741 a         5243 c         5230 g         6270 t
ORIGIN      
        1 ttttcatatg agcaatggat tcgttatttc gctgtaaaat attcaataat tcattatgga
       61 taccgggctg cagctttttt ttgggggaag ttaaggcaaa aaaataaccc ttggttgtag
      121 atcaaagtga ataatcacta aaagcaagct tctcaatcgt attcttgccc tgcatttgcc
      181 ccaatataac ccaattcata gtacaacatg atccgaaaat gagagcaaat ggctggggat
      241 ggagaggtga aagaagaaag gttaaaggac gaaggtcgag gaattatttt gaatccgtaa
      301 atctcctttt taaaatccac tcagattcca cactattgtg cctgcagacg gtcaatcttc
      361 tgcaggcgtt tttttaaaag cctttattcg tgatggtctc acggaaaaaa cagcctattc
      421 aggctattcg tatagagttg atcctttttg gatgaccttg aaccgttttt cacctatcat
      481 aaggtaagca attcgttttt caggatcagg atttgggtca atagaatagg ttataaagcc
      541 tttgccagcg tgaacctcat caccctccat aattcttagc cagctctggt cgctttgtac
      601 tgaccactca cactgaggct gggttctgac cctgatcttt ttcacacccc ccgttgcatt
      661 gaacactctt tttttcttaa aaagattgta tgtacaggta gttatggcta cttcatctga
      721 gaaatcactc tcctgtcctg ccccattcaa agcggttact gcaaaataat aattttgccc
      781 ttttttgaga ccgcttatct tttttaacgt ttcacctaca atctcaacca taaatccata
      841 atttctgctt tcattgccgt aataaagccg gtatcccaca acattctccg caggtgaatt
      901 tggatcccag gcaagtgtca cagatgtacc atgagcatag tgggcagatg ctgtaatggc
      961 tgaaaaaatg aggataataa agaagatagt ttgagtagtt gaacgtttga tcggtttatt
     1021 gttggctttg ctgtgatgca tataagcacc tcctgaacac ttctttagcc ggcgaatagc
     1081 cagcactgga ggtgctgttt tcgcctggca accctgctgc cgtatcctgc agaaaggact
     1141 taggccagta gttttgcgtc ccagtttttc aactggtttg cctttgtcaa gctatggtca
     1201 ttcataacag gtttttttgt attaggttcc acaatgtttt gcctctgttc attatgacca
     1261 tgatatggtt gtcggttttt ttgaattaaa aaaaatgaca aagatcacaa aaacataatt
     1321 gttgatgaat agacgataaa tcacaaccgc cccctggtct ttagaatcct ttatgcccag
     1381 ggggcggttg tgtaatcagt atctactgtt ggagagaggc gtattggtcg gtgtatgatg
     1441 gtggcggaat cgacgtatcc tcgccagtat catatgaatc aaattgttgc gcctctgata
     1501 tgtcgcgatc ctgaatatcc tgcaaaacta cttgttttgc aatggttttt ttaaaaggat
     1561 tctgaataaa ttttgcctga gattcaaacc tcactcttac gcgatgatta cgtttgatat
     1621 tggcaaaggt caatgtatcg acagcctcaa ttggcttacc attcacaata atctttttaa
     1681 tataatgttc tggctctggg tttatgtggt aaacaagctg gcttccgtaa ctgacggtga
     1741 tagcctgttt acttcgcttg atttttgtct tattcttgat ctttttattt ttaatagagg
     1801 ctagcccttc tactgatatt gagccatttc ctatggcggt tgcaattaat ttgaaatttt
     1861 tctgttcaaa tgctgcattg atttcggcgt gctgattcat tatcaatgta cattttgacc
     1921 tattactttc acaatcacct gagaagccac taaaattaga cccttctccg gcaatagctt
     1981 ttagtgttat ttttgttccc ggggcatagg ccgttgctaa aggatgggca acaacgtgtc
     2041 cagagccagt tccgcttgtg gtgatcgata aagtatagcc tgggacgaaa agagcataaa
     2101 ttgtgtgatc accagtgaca tttcgaaatg tatatgaact gacagctccc actgaggacc
     2161 cgtcaaccaa aacatccgat attgcatggc ttgcatcagg cgaaattgtg aatgtttgac
     2221 tgcctccatt cggtaccgaa atactttctg atggcgaaat cgtccctcca ggccctgctg
     2281 atgcagtgat tgtaaaacta ttaactttaa aaaccgctga aatagtgtgg ttggatgtga
     2341 cattactaaa aatatacgag ccggtagctc cctgagaaac cccatccact ataacatctg
     2401 ctatggtgaa accgttattt ggtgaaatca cgtatgactg atttgcccca tgatttactg
     2461 atttgttacc ggatggggag atgcttccac caggtccagc agaagcgttt attgtgtagg
     2521 ttttgacaga aaaaactgct gcaattgtgt gatctttagt tacattgcta aacgtgtaag
     2581 aactggttgc ctcttgcggg acgccatcaa tagcgacata tgaaacctga taacctgcat
     2641 taggggtcac agtaaaggat tgatttgccc cctgtgctac agtcactgat cctgagggtg
     2701 taatgatgcc tccctgactg gctgatgctg tgatcttata tgttgtagcg cccgactgtg
     2761 caactgtgaa taccttgcct gcgaaagtgg agcttgcagt ccgtgcgtct gtattggtgt
     2821 tggctgacac tgaatagcat atgacaccat tccctgtccc gtttttgcct gaagtaatag
     2881 tcaaccagga agcaccgctt gaggctgtcc aggtacatcc tggctgtgtg gtgacagata
     2941 cacttccggt accaccggaa gcgctgaaac tcgcattcgt ttgggaaatc gaatacgtgc
     3001 acatatcttt atttacttca tcagaatacg tgctttcaat gggcggattt gaattatcat
     3061 aggctgtagc agcaaaataa taggttgacc cattgggaag gtcagttata gtaacctggg
     3121 ttatgttgcc aacatcgatg aaccaatcat aattacggct tgctttgccg taatagattt
     3181 tgtatccagc cacgttaggc tcattgttca tatcccatgc aagcgttacc tggccggcac
     3241 taccaatgtt tggagtgaga aatatagaca atgacgagag ggcgagtatg aggactaata
     3301 aaggacgagt tatcatctgc aacacctcct gctggattca attggcctag cgaaatccag
     3361 cactcagaga tgctgccttc gcctggcaac cccgctgcca tgtcctgtca tcaaaggatt
     3421 taggtcggtg gctttgcgtc ccacgctttc acgtggtttg cctttttcaa gcaaatcgtt
     3481 tctatgatat gattagtatg gccggttggg tggacgaact aaatgatgaa aatcaccgat
     3541 aaaaactgac gatagaaatt cagtcagtac agcatcagta cgcaaaagat aaaccatact
     3601 tggcggaaag ttcgcacata agaaagttcg cacataaaag gggccgcgtt gtatgaccgg
     3661 atatgccggc aaaccagagc aaaaaccgat gaattgttat tgtcgcattg acaatgccgg
     3721 gtcatatcgc atacattata taatcacgga agtgccaggg tcctggtggg cctcctggtc
     3781 ttcaaaacca gtgcgccggt gacgagccgg cgggtgagtt cgattctcat gcacttccgc
     3841 catcagtaaa atcagtcagt taaaaacctg agtgttcaac cataagctgc aagaataata
     3901 ataggctggt tatcgtaaaa catattacca gactccagaa ctagaaattc taacatgaga
     3961 gaaaaacagg gctgcctgga atgggcatcc gtctcaagcc actggtgtgg atatgaaaca
     4021 attcttttcc agtttccgaa atgtttgccc ctgtcggatc taccttccat ggtatgttat
     4081 ttacagttgg cggggtatgc gtgaacgtgg gcttcttgca cctgccggca tgagaaagag
     4141 aagaggaaca gcaacaagag gcatgattcc aaaggaaata aacacaatca taaatccggt
     4201 aaaagcatcg gggctcagat gtaatacaaa ggtaatgaga gagatagcga gtgcaccgcc
     4261 tatattcctg aacatattcc tgaggccgac gatcgtaccc actttctcag gcatcaactc
     4321 tatgcacgcg ttattggcag agggaaatac catccctgca ctgatcccat tgagaaacat
     4381 aataattgag aatgttccga tgactcctcc aagggcgccg ccagcgctgg tcaggagaag
     4441 gccgggtgtg agcaggataa ttccaagccc catagtgata aaacctatta ccattggcca
     4501 tcgatagccg agtctcctga ggaggaaact ggttgcagca gaggtgacag tcattgccag
     4561 ggagcgaggg gtaagaatca tgccgctcat taaagtggaa aatttatgaa cagatatagc
     4621 atagagaggg atgaaagaga ataaacccat agaagtcatc cctacaatga gatttgatac
     4681 atttgctgcc acaaaagccc ttgattcaag aagcttggtg tctatgatag gcgcatgggc
     4741 tcgcttttcc tgtttcagaa aacagatacc aagaaaaaag ctgatgaaca ccagtatccc
     4801 cgcaagaatc atcgagacgg ccgagggcct ttcgccgacg agattcagtc cgaggaggag
     4861 caacaaaaga gaactactca tgagcccgat gcctataaaa tctactcggg ggcgggtaat
     4921 gttcttggat ttcttaagga gcatcatagt caggcagatt aaggcagcac ctatggggac
     4981 gttgatgtag aaaacatagc gccaggagta ctgactcaca atccaaccac cgagatttgg
     5041 tccaacaaca aagccaaccc caaatatgct tgacagaaga ccgattgcag tttcacggct
     5101 ctcaggaaaa aaatcgctgg ctatgccgga tgctgtggga agaaaacaag cacccccaat
     5161 tccttgaaag aatctgaagg ctatcagagc atagatagag ctcgcaaagc cacaggcaag
     5221 agagcttcct gtgaagacaa gaagggaaaa aagaaacacc ttcttacggc cgaagctgtc
     5281 gctcagattc cctgcaaggg gcattgtcat ggtgacagca ataaagtaaa tcgaaattgt
     5341 ccatgctgac agaagaacgc ttgcatggag ttccttggtg aagttaggga aggccacgga
     5401 gattacagtg gcatctatgg cgtacattag gatgctgacg ccggcaataa agaaaaccag
     5461 gtacttgcgc atcaggacga cactttctgt tacaagatga cgggagtcta ctaaattgcc
     5521 tgataaccgt caacagaaat caggatcata aaaggataac cagaagtata gcctttatca
     5581 taaatgatgc ttaagataca tgcatcttat attagaagga cacttcatga gacagtgtcc
     5641 attcacggaa aaatgaagga gataggacgg tgaaacgagt aggaatggct gttttggcta
     5701 tggcaatttt ttttggtttc tccggaatag tgtgtgccca ggaaaagagc tctgatgtga
     5761 tccgcagatc atatagcgtt tacagtacaa accttttcca gggaaagcag ttactaaaac
     5821 agggtgattt tctgtgggct cgagcgcatt ttgcgaaagc cctggagagt caaaagtggc
     5881 ctgaagccgt cgcatttgca gcaacggcaa gctacaagat gaatgacatg agggctgcgg
     5941 aacactatat tgcagatgcg gagaaaatga ctggcaaggg tgtctttgag ctgcggattg
     6001 ccggctacaa agcattaatt tatctcaagg aaggcaggca ggatgaaggg ttaaaagccc
     6061 tgcgggagta cgtagccctg tataactacc tcgatccgct gatgagtatc agggaggtta
     6121 aagccatggc aataaaagga atggtaaatc taagtatgct ggaagcattg ttggatgaac
     6181 agattatctg gcgtgaggat gagatagagc agtacgagaa aacagggaca gggttttatg
     6241 gaagatacgg tgggccataa gaatcacaaa acaggtatgg atactatcaa gaattggagc
     6301 atcctccaaa cccatcctcc aaaaacatca tagggatata cgaaagacga ggagcgatca
     6361 tattctgatg ttcttctgtc acactggaat atggctgaca gcgaacaagg gctgaagctt
     6421 gctggttttt acaacaactc tctgtgtgga ataacagggc atgcttcatc gcgaaacatt
     6481 gtatctttgg agggtttacg tgacacacag tctcacttgt gccgatgcaa acggtggagg
     6541 cgatgttcaa gcgattcacg ttcagtgtcg ctcaagtttc tggcttcgtt caagagctta
     6601 cgaaccattt ccagagcttt tccgaaattg cgggtatgat gctcgtagaa tttggccagt
     6661 tcttctatcg caaaaacatc gtggggattt attgccacaa tttcctgcca gagtgctatg
     6721 gcatctttcc atcggcgggc tttcttgtac ataagagaga gtgatcgccg tgcgcctatt
     6781 gccacgtcta tacgattgga ctcgagtagg gattcgagca tcttctgtgc cttttcaggg
     6841 ttaccccgtt cgtagaggag tatggccatt ttgaggaaat caatgtgttg tccctgtcct
     6901 gcctcctgac aggtctctgt aaggtctgcg aggtgtttca ggagtgatgc catagatatg
     6961 atgtccatcc tgttgtgtgt gaaaacatcc tcaagtaatt ttccatcgcg cactcggagc
     7021 cagtcaaaat agcgctgggg tatctcgaag ccgggcacat ctccatttcg ccacaccccg
     7081 agtatattcg tttctatggt tgaaagacgg acgttttcta ggcggtgggc aaagactctc
     7141 ctgcttggat ggagaagatc aatgtgaggt ttcactgtca gtgtgtcttg aaaacgattc
     7201 aggatgaacc gtgttgccag caggttcaga tcataagctc tgccgttaaa ggtgacaagg
     7261 aattgcttgt ccgatgcaat gtcgctcaga tagcgcagca tggccttctc ctcagagaaa
     7321 tcccgtgcaa agagctggca ggtgataaac gaacctgcat cgaaccaccc gagcccgatg
     7381 aggaagggaa aggtgccggt gcctcctgcg aggcccgtgg tctcagtatc gaggaacagg
     7441 ccgtcttcta atgccaggtc tatggtcatg ggcgggtagg agagaaaggc tgttgcctcc
     7501 atattcaggc atgcaaagtc agagatatgt acatgcccgt gactatcatt tcctttcagg
     7561 ctgttgcggg atacgaaaag attaccatgt tgagtccgga tctcctcgcc ggcaacaaca
     7621 tgctccagcg gtattgcctt ttgccatgcc tccgcaaaac gtggccgggc aatgcgctct
     7681 cgccggctca tgatctcgtc tatttttctg cgaagttcgc cgattgcctt ttgcggggag
     7741 tcagcctggt tgggtatggc aggttcgccg gttaatcggc ccaggcggtc tctgaggctc
     7801 atggcgtatc cataagatcg agaatcttaa gtgctgcact cttggctgaa ggcccaacct
     7861 ccatcagggg ccccacgcac atggggcagc catgttcgca cgggcacgag gtaatgacag
     7921 accgtaccgc ccgtacaagc tcgttgtgcc tttcgaacaa attttctgag aatccgatcc
     7981 cgccaggata ggcatcaaag ataaagacag tagggtcata ggcgttttcc atgacattca
     8041 caggtttgct ggggctcgta gttatcatcc tccggttatc accgcgacgg acataccatt
     8101 caccgctctt gtcaccaata caccaatcga catcacggat atccgacatg acgagcatgg
     8161 cggcaatgtg gtggaggcag taagtaagac cggcaaggcc atcaaggatg tccaggcggc
     8221 tgagttgcaa cgaataaagg aggtcccagg ggatggtgaa ccagtagctg gttgtgtgca
     8281 tctctcgttc aggcagatct acatcgccgt agcccacgtt ctcggaagtg tagaacttga
     8341 tcttcttgta gcccacaacc ttgcgcgtta cctggacctc gccgtgctgg acaatgagcc
     8401 tgcctttcat ctttgtgtcg aagtcgtcca taacgcggac accggtatag gtcatggcat
     8461 cggtgtagta atcgacgtcc accttcttaa catacgcctt cttgcgctcc agatcgagtt
     8521 tgtccacatg gtactgttgc gactctacca tgtagatcgc gctgtcgtgg actgctgtga
     8581 aagcgctgtc ccagtctact tctgctatga ctttgtggtt tcctgtatcg gtggtgtcaa
     8641 tcaccacaat gttttcagga ttgaccgttc gcaggctcac ctcgtcagca gggtagctct
     8701 ctgcagacca gtgccagcgc tggtctaccc ggtggatcac tcctttttcc tccagatagg
     8761 tgagcaactc cacaagatct tccttgccaa atttttcacc ttcttcaaag ggaagctcaa
     8821 atgctgcact cttgatgtgg tggagtagaa tcagcagatt atcggggttc acccggcagt
     8881 gctcaggaga gcgggaaaag aaatagtcag ggttttccac aatgaactga tccatgggat
     8941 tgctcttagc gatcaggatg gcaaggctgc tgcctgttct gcgtcctgcc ctgcctgcct
     9001 gctgccaggc actggcaatg gttcccggat aacccaccat gatgcatgcc tccaggtcgc
     9061 cgatgtcgat gcccagttcg agggcattcg tacttaccac ccccatgatg ctgcgttcac
     9121 ggagaccctt ttcgatctgc cgcctgaggt tgggaaggta gccaccgcgg taaccggtca
     9181 catagtggtc atctttgggg attttgcttt tgaacgtatc cttgaggtac ttggtgagca
     9241 cctccacatt aagcctgctt gtggtgaagg caatggtctg tatcctgtta tctatgaggt
     9301 tgctcgctat ggtacgtgca ggggtcagag ctgactggcg tatgcccagt tccctgttca
     9361 cgataggcgg gttatagagg ataaaggtct tggcagatct aggggcgccg ctttgatcga
     9421 tgagaacaac ttctttctcc aggaggtctt ccgcatgttc cctcgggttt gccacggttg
     9481 cggaacagca gatgaaaacg gggtcctgtc cgtagaagcg gcagattctt ttgagacgcc
     9541 gaaacacatt ggtcacgtga ctgccaaaaa caccccggta gatgtggagt tcgtccacga
     9601 ccacatactt tagattggaa aagagtttct gccacttggt gtgatgaggg agaatgccgg
     9661 catgcagcat gtccgggttg gtgaccacca cgttgccctg tttgcgaatg gcctgcctcg
     9721 catcgtctgg tgtgtcgccg tcgtaagtaa aggttttgat gtttgctttg agttccctga
     9781 tgaggccgtg aatctctgtc atctggtcat tagccagtgc ctttgtgggg aagaggtata
     9841 tagctctggt ctcaggctct tcaaggatct tttggagtac agggatgttg taacacaggg
     9901 tctttccgct ggctgtaggg gtgacgagga caacgtccct gccctgatga acaagctcaa
     9961 tggcctgtgc ctggtggctg taaagcttac cgatgccctg cccggtgagt atcttttgca
    10021 acagagggtt cacccagtgg gggtatgggg cataactgcc ctgcaatgcg gggatatgcc
    10081 tgatggctgt tgcattttcc cggaaacgcc tgtccttttc agcgcgggtc agccactctg
    10141 ttaggcttat gtggggcatg tccctgcctc gtcgccttgt aaggtgagtt gctccttctg
    10201 ctcaggggtc aggcgaagac tgtctatcgc gtgctcggtt atgaagtcta tcagtttttc
    10261 ttcagtgtcc agattttgat tgagactcac tgtcctcaga atgctatcgc acgggggaat
    10321 gtccctggtt ttttgtctta cacccggatc gtcactccgg gagagggctt gccgtattgt
    10381 ttctcgaact gactcacata tggcaacctg cctcacgtag ctaatcattg caaaacacct
    10441 ccccataaaa aactatgacc attcatgcag atgtgctaat ggttctgttg tgccaatatg
    10501 gatatgccac attgatacat actatactcc aactggcgag tgaatggcaa tctacacact
    10561 cactcgcatt cttttggctg tttttgaaac aggtccggta tgaaagccca acagagcaga
    10621 gaggttccag ggaaagagtt ttattgactt ctttctcatt tcgtgtgata aggtgtcata
    10681 cagaaagctt ttgagcttca attcaacagg aggggtgctt acgcatggca aaaggaaaag
    10741 tgaagtggtt caacgagtcg aaggggtttg ggttcattgc ccaggaggat ggaaaagatg
    10801 tgtttgttca ttttacagcg attcaagggg aggggttcaa gacgttgtca gagggcgacg
    10861 aggttgaatt tgatgttgaa aagggtcaga agggaccaaa cgcggtaaat gtaaagaaag
    10921 tgaaagccta aaaaggcagg gcagggaagg gcagggacat cggttagtcc ctttttatga
    10981 tgaataccag cggaaagctg atcacacctc ctgtgacaaa caatgggtag tgacaggttt
    11041 tgcccttact tctgatgggc tttgtacacc ctgcatgcaa tgcccctcaa gttcggtgac
    11101 cccatctcag cgccccacgg ttgagaatcg tctgtcagga tattcacgtt tgcctcctcc
    11161 caaccgtaca taacttcagg gcccctttcg ggaaaccacc atccatagtc cacacccact
    11221 acccgtgagt ctatatcttc tgttatcctg gccttttgtg ttatcctgcc ctgtttcgtt
    11281 tcgatataga ctgtatcccc ctgcgtgatg ccaagtgcag tagctgtgtc agggtggatc
    11341 cagacttctg gctcatgatg gcgttttctg agtgattcaa tctgtctttg actggagtgt
    11401 ctgaattctt cgactttcca tgaggttaaa acgagaggat attccctggc aatttcaggt
    11461 gaactcaagg gtgtatccgg aatttcccgg tatataggta tgggatcaaa cccccagtct
    11521 ttcaagcgcc gggaataaag ttccactttt cctgaaggtg tgggaaaacc gtccttctcg
    11581 tgcctgcggt attctttttt tcccaccagt gtaccaatct ttttgaattc gtcaaatgtt
    11641 atacctgcag gttttaaaat gaaatcaagg cattcttttt cggtttccca gaaatattca
    11701 ccaataccta atttccgggc aagagctgtt ataatctcag aatcagagcg gcactcaccg
    11761 acctgcgcta ctttttgttg tacctgggcg agagggtagt agggtggcgc tataatgttg
    11821 tttatttcga gaaaactgcc cacaggaaga acgatatcag acatggcggc tgtaggtgtc
    11881 atgaacatgt cgattgttgc agagaagcca atcttctgta aggccttata gacatgacga
    11941 gcatttgaat aggtctgaag catattgcat tcaaaaatca atgcgctatg cagcggatat
    12001 ggttcccctt tgaggatagc ctttactata ctctgaggga gtgcataaaa aaccgatgga
    12061 agaaatccat cagaagcatt gagtcgcctg ctgcgaacat ccggcaaaag cttgtctctt
    12121 aagtcgaatt caggtgatcc catgggtaca attgccggtt gggagcaggc aagctctccg
    12181 cccggaacac caatgttacc ggtaattgcg cgaagaattg ccagagcacg tgcagtctga
    12241 aaattgttgc ctgtatggtc gattgcattc cctgcctgta taatcgctgg tccggctgtg
    12301 gcgtagagtc gggcagcttc tctgatgagg ttcgctggta tccatgttac tttttccact
    12361 atctccggag tatactgggc gatatgctct ttaagttggt cgaagccaac cgtccatttg
    12421 ccaacgaaaa cccggtcgta aaggccctcc gtaataatca catggattaa gccaagcgcc
    12481 agagccagat ccgaacctgg gcgaacttgc agccagatat ctgctttccg tgagaggtcg
    12541 aacttacggg gatcgacaac aatcaacttt gacccgcgct gcagggcttt caatgtgtcc
    12601 attccctctc caaggcgggt ttcagaaaga tttgctcccc ataccatcat gcaggaaggc
    12661 ggataaccat aatcgggcac tggattgaag ccatttgtca gcaagcttcc gttgactctg
    12721 ggtatgaagc agacaggtgc catcgaggca atattcggtg tcccgaagac attggcaaaa
    12781 cgaccaatat acccgccctc atagcctcct cggaatgatc ctctgatgaa aaccacactt
    12841 tcagggccat atgtttcctt tgtcttcttc agtgcttctg cgatctcatt aagggcttca
    12901 tcccacgaaa tacgatccca tcttccattg ccacgttctc cagaacggcg cagtggatat
    12961 ttgagccggg cagggtgata aaggtgctca agagcggtct gtcccttcag gcaaagccta
    13021 ccatgattga aggggctttc aggatcaccc tctaccctaa caatttcacc attctgtata
    13081 tggacgagaa ttccacaccc agcctggcat aaaacgcaag cactttttag aatttctaca
    13141 tcgttcatgt tacctctttg tatgtcctgt cgggcacagg tatgattctg ctcgattcat
    13201 atcatgaggg cttccttggc ttttctggat tcaatgttga acagcactgc atgaagcggg
    13261 ttctcatgct tttcattcat cgtcaggagt cattttttgt ccttcgtata ctaatattat
    13321 tctagtggta gtctaatatt ttaaagagaa ataataaagt attccgccag cacaacgcca
    13381 gaaattgtga ggttaagaga atattagagt ctggagaaca aaatattagg attgacattc
    13441 ccggtctatc actattttgg tataatgttt attatgaaaa aaacaaccct tctttttgca
    13501 gcaattatac tgttttctac tgtgttaaaa ttacacgggg cggaatggat atctataggc
    13561 aaggacaggt tgggtaatga attattctat gaccatgaag ttataattga gcgctcaaaa
    13621 gatataatgg aagtacagat gaaggggata tattctgatg cagggagaag ggagagaata
    13681 caagacagaa ccagggcaaa atcaactgtt gaaaggtatg aaacattgag ctactccctg
    13741 gaattgcagc aaataaattg cgtgaagaga gagtttcggg taatggctta tactgactac
    13801 tcctctgatg gtggggtact ttacaaattt atcgcagaac aacaagcaat gaaaggatgg
    13861 gagcccatag ccccagactc aatgggggaa aggatgtaca aagctgtctg ccggccatca
    13921 tcagggaaaa agaactgaat aggaaaatca cataagccct cttctcaaaa accatgtaga
    13981 tagaataggc agacagcaca ttgacatgcc tggtgcatgc catgctgcgt ttaagattat
    14041 tttttcacaa attctgctga aatcctatgg ttcttcgtca cattgtcaaa acgataatag
    14101 gtaacagcac cctttgatac accattaacc aaaactcttg ataccctata gccggtattc
    14161 ggacgaatcg aaaagtactt cccgtaccca tagggcacag aaacggatcc tgatggagag
    14221 attgtcccac ctttccctgc tgatgcagtg atggtaaatg ttttgagggt aaattttgca
    14281 ttaatggtat gatttgcaga aatgccggta aatgtgtagg agcttattgg acctttccag
    14341 cttccatcta cgtaaacagt gtatatatca tagttttggc tgggtgagat ggtaaaagac
    14401 ttgcttgcac cacgagatac tgcaactgcg ccggatggtg agattgtacc accggtttct
    14461 gctgaagcag taatggtata ggttgaagat gatactggca cgctgcttac aaagcttgct
    14521 gcaattgtat gatttgctgc tatattgtga aacgtgtagg aggtaattgc acctaccggt
    14581 gtgccgtcaa ccacaacact cgatattgca tatccggggc cgggggtaat gaagaaggtc
    14641 tggcttgttc ctgctgactt attcacagtg cctggcggtg agatagtacc tcctgttccc
    14701 gccgatgctg tgatgttata gtaggttgag cctgtgccag cttggtttat tgtgaacagc
    14761 ttgcctgcaa tagtagatgt agcagttctt gcagtggcac cggtattggc agatacagaa
    14821 taggatgctg taccattacc ggtaccctgg tttcccgatg tgatggtaac ccaaggcgct
    14881 ccgctgctag ctacccaggg gcaaccggcc tgggtggtga cctggactgt ccctgttgac
    14941 ccttgctgtg taacagatgc tgaaggtggt gagatggaat aagagcaggt cctatggttt
    15001 acctccaccg aatatgcgct ttcaacgttt gacgtgctgt atgctgttgc agcaaaataa
    15061 tatatgtttc catctgtgag tccagttacc gtatacgtat tgaccttccc cacgtcaata
    15121 aacatggtat aatttctgga ggcgtttcca taatagattc tgtatcctgc aaggtcactc
    15181 tcagtgttgg aatcccaggc gagagtgacc tgggccgcca ttatcggcac tgcaaaaaga
    15241 agaacaacta atcctgctgt gaaaaacaga aaagaaaatc taacacatat ctgaaagact
    15301 gcattggctc tatatcgttt ttctgaaata cctgttcgcc acaaactgat catatctggt
    15361 cttgccattg aataaaatcc tttctcgtat cgtttgtttt aaattagcaa gtaccatacc
    15421 tgggaggaaa tctgttgtaa ttaattgata ttaaagatat atattgtcat tactgtcatt
    15481 tgtggagatg ataaacgaat tgccaaacat tcgttcttaa tggcaggaat gaagtttttt
    15541 tcatacagtt tttaacgcac ccctcctttt ctgttgctcc tggtatcgcc agatcctgtg
    15601 tagagaatgg ttctgtcatc tttactgtat tgaacatcga gcacaaaggg gctctccatc
    15661 aagggctcat atatgctatt tgaaaatggc tgctccatcc aaaatgtaac gccagcccga
    15721 ttttcattac aaactctggc caaaagaggc tgaccaagga agtgtatccc aaataagcaa
    15781 taagggaaca gtgtctggtt ttctgtacac aaaccattga aaagaaggga aacctggcct
    15841 tgttaataaa gccccgaaac taccacgcac attgggtgaa agaaggagac cccgacagct
    15901 ttactggcag atgggagatg tgaatccaag gaatatgttc cccggctaaa ttaagtgaat
    15961 gcagtgaccg ggacagcatg agcgtctttt cggtgaatac ccgacccata tgcccatcct
    16021 gtatggagtt aagattcaga atctgatctt gttgatactc ttcgttgcct tacgcagctt
    16081 tttcggtagt tttctggggt tgcctgtttt cgggcgtcgg aagccactat catcgtcttt
    16141 ttcgtccaat tcctctttgc cgctcttcat gctctgttcc atggcaactg gggtcatgaa
    16201 gggcacacca gccttcttcg cttcgtcgat aatggcgcga tccgaggtcg ctatgatctg
    16261 acccgagcgc cgctccctga tccagccgat gatcacatca tcggcagtct caccttcccc
    16321 tgcatagaca acgttaatac ccttgtaact ctcccgttgc ctgccgaggt tatagcctcc
    16381 gcgggcatca aaaaccacag tgatacggac acttcgatct cttttatatc gaaatagttt
    16441 ctcaagaagt tcccgtctga gcatgtccag gtttgagctt ctatcaattg gtccgctccg
    16501 tatcctgtgt atgtaattgt atccgtctat tattaggtga agcatgcgtg aatctcgatt
    16561 ttagctatta gctgtgagct aaaagctgta cactaatcgc taacggctga tttcttataa
    16621 gactccctta aaatcctgtc gagaatcccg ttgatgaaat ctctggattc ctcattcccg
    16681 tactttttgc ccagttctat ggcctcatct attgcaacct tgggcggcac atccggagta
    16741 tagagcattt caaagacagc cagtctcagg atgctcctgt caacgtatgt tgtcctcgca
    16801 agcttccagt gctcgctcgc attctcgata cttttgtcaa tagctgcctg ttcctgcctg
    16861 actccagata agagtaaccg ggtataatct aatatatctt gctggtaagg aaaggattca
    16921 cagtatcgga ccagagcaag ttccggatcc tcgcctgcgg tctccacctg gtagagcatc
    16981 cgcagggcta gctctcttgc tttccgtctc cgcacaaggc tttgtccttt aaaagatttg
    17041 ccatttcaat ggctgccatt gcagcgtcat aacctttatt gcctgatttg gttcctgcac
    17101 gttcaattgc ctgttcaaca ttttcgctgg tgatcactcc aaaggtaatt ggtttctcat
    17161 gatcaagtga ggcaagtgca atgcccttcg caacttcgga tgccacatag tgaaaatgag
    17221 gagttgcacc cttaataaca gcaccaagac agataacagc atccacatct tttttctttg
    17281 caacaagttt tgcaacaagt ggtatctcat aagcaccggg aaccttataa atatcaacct
    17341 gcttatcttc tgctccatgc cgcttgagtg catcgagagc accatccaca agcctatcag
    17401 agataaagct gttaaacctg ctcacgacaa ttgcgaactt gaaacctttt gcactgagct
    17461 tgccctcatg tatcatattt gctccttata tgttgttaag aatatggcca agtttttcct
    17521 gtttggtttt taaataggtt atgttctctt ttgtgggctc catctccaga gataccctct
    17581 caacgactgt aagcccatat ccctcgagcc ctcttatttt tctcggattg tttgttagga
    17641 gcttcatttt tcggacgcca aggtctcgaa ggatctgtgc tccaacccca tagtcacgga
    17701 gatcaggtgc aaatccaagg gcaatgttcg cctccacagt atcgtgaccc tgatcctgca
    17761 ggcaataagc tttgagttta ttgacaagcc cgatacccct gccttcctgc ctcatataga
    17821 ggaaaacacc tttgccctcc ttctcgatca tcttcagcgc cgcgtgcatc tgttccccac
    17881 agtcacatct catagagccc agaacatctc cggtaaaaca ttcagagtgg accctcacaa
    17941 gcacaacatc atctggcttt atatcaccct tcacaagggc cagatgttct tgtctgtcca
    18001 tatcattctc ataagcaatc aaattgaatt caccaccata ccgtgtaggt atcttggtct
    18061 ctgccgccct tgttacaaga tgctcattcg ccattctgta ctgaatgaga tctgcaacag
    18121 ttccgatttt gagcccatgc tgctttgcaa atacctcaag ctggggaagt cttgccatgg
    18181 tgccgtcttc gttcatgacc tcgcagataa ccccagcagg ctttaaaccg gcaagtcttg
    18241 caaggtcaac agatgcctcg gtgtgtccga ctctccgcaa aacaccaccc tttcttgcta
    18301 taaggggaaa gatgtgtcct ggccgtgcaa ggtcatcttc tgttgaatta tcatcgatcg
    18361 ctacctgaac cgttcttgcc ctgtcatggg cagaaatgcc cgttgtaaca cctttctttg
    18421 cttcgatgga aatggtaaaa gctgtattat gctttgatgt gttctcccgc accatgaggg
    18481 gaaggtttaa tgccttagct ttttcgtcgg ttaatgagag gcagataagt ccgcaggcat
    18541 accgcgccat gaaggtgatg gcttcagggg taactttttc agcagcaatc actacatccc
    18601 cttcattctc tctatcttca tcatcagtca tgatcaccat tttaccttcg gcgatatcac
    18661 tgatgatgtc ctctatcttc gaaattgcca tgattactcc ccttttacat atccatgtct
    18721 gtaaaggaag tcgagatcaa ctcccttgtt cttgtcctta atcaagaaac tttcaatgta
    18781 ttttccaata atgtccgtct caatgttcac tttatcccct ggattctttt cacctatggt
    18841 tgtcttggat gtggtgaaag gtattatgtt aactgtaaat atattatgcc tttgctcgtt
    18901 gactgtcaag cttattccat cgatggcgat cgatcccttt ttcacaatat atcttgcaaa
    18961 atcaaggggt atttcaacgc tgaatctctg tgaatcgcct gccttcctta tatccacgat
    19021 agtgcctatg cagtcaacgt gccccatcac aaagtgcccg ccaaacctac catcggcccc
    19081 catggccctt tcaatattca cccttgtgcc ggtcttcttc tcggccagtg tcgttacctt
    19141 tagggtttcc agagatgcat cggcaacaaa accatctcct gttattgcag tggcagttaa
    19201 acagacacca tcaatggcca cactgtcacc ttcccgtatg ccagctttgg caagcgctgt
    19261 tttgatgaaa aattcccact tcccactcga ttttttgata gaaagaatgg aaccgatatc
    19321 ttcaataatt cctgtgaaca tatccttcta cgtagatatc ttccttaaac cgctttacag
    19381 cagctatatt gattttgtag gcatccttga gaaaatctat ccctttgccc ccaatgaggt
    19441 tgtaggcgcc tttgccaccg atgagaatag gagcgtaaaa gagcatgatt ttgtccagaa
    19501 aaccttcctt caggagacag ctattgattt caccaccacc ctccacaaga acgtgcataa
    19561 tctctcgctg atagagtact tcgcaaacat gttttggcga tactctcccg ctttcgtctt
    19621 ttgatactct tatcacatca agccccatgg ctctcagttt tacctctttg tcagggcgtg
    19681 aatcttcaga tgtaaatatc caggttttat acttctcaga ggtcttcaca agatcacacg
    19741 acaaggggat acggagtttc gagtcgagta cgatgcgaac aggatacttc ctcggtcgag
    19801 cgatttttgg cacaagaagt gggttatcca ggataacggt gttgatgcct accatgattc
    19861 cgtcgacagt agctcttacc ttgtttacaa aacgccgtga ctcctcgttt gatatccatt
    19921 tagaatcaca ggtctttgtt gctatctttc catcaaggct gacagcaccc ttcatggtga
    19981 agaacggtct tttcttctcc atgtacactg cgaaggcttc atttaatctc tttgcttctt
    20041 cctcaagaag tcctactttc acctcaatgc cggctttttt cagctcattg accccttcac
    20101 cgtttaccag tgggttcggg tcaagcatgg caaccactac ccttctaacg ccggctgcta
    20161 tgatcgaatt cacacagggt ggagtcctgc cagcgtgggc acagggttcg aggttgacat
    20221 acagggtagc ccctttagct tcttcttttg cttcgttcaa ggcaacaatc tcagcatgag
    20281 gtgtgccggc tttccggtga tacccttttc cgacaatacg gttacctcta accagcaatg
    20341 cgccaaccat tgggtttggg gatgtcattc ccatgccttt tcgtgcaagc gataaggcca
    20401 ggtgcataca ttgttcgtct ttcaccgttt aacctctttt aaagaagcac gtagctgcct
    20461 gctgacagct taagctcaaa aagtaaagag aaagaaccat acactgtttc ctgaacggtt
    20521 gctctcatca tggagattca tccttcttaa ggaggagtcc tttgagctcg tccatgagct
    20581 cgttaatatc cctgaactgt cggtagaccg aagcaaacct gacataggca atatcgtcga
    20641 gccgtttaag ctcctccatt accatggagc caatgtctga agccttgatt tctctctccc
    20701 cacggtccaa aagtgtgtat tcgatcctgg aaacaatttt ttcaaggtgc gtaatactta
    20761 ttggccgctt ctcgcatgcc tttttcagac cattcaggat ctttagcctg tcaaacactt
    20821 ctcgcctgtc atcttttttt atgacaaggg gtagcccttc ttcaagcctt tcagcagttg
    20881 tgaacctttt tgcacattta aggcattccc tccgcctccg gatggtgtcc atctccttgc
    20941 tgatccgtga atcaaggact ttactttcta aaaaaccgca aaaagggcat ttcatgcttt
    21001 cttcgaacgc agtttaaagt ttttgacttt tacgtgctgg ttaacacttc taactgagac
    21061 ctgtcccggc caggccggga attctgaacc ctgtactgtt ttatcaaggg ctgatttttt
    21121 ttcaaaatca atcttctgca ccacaattgc tgactcaagg atgaattcat gggagagttc
    21181 atcaggatac ccgtctgcgt aaagcacttt tgcgatgccg gcgtttatga tcatcttcat
    21241 gcatattgag cagggaaggt gggtggaata gatgtcagcc ccattgattg aaacaccatg
    21301 atacgctgcc tgtataatcg cattctgctc agcgtggaga ccccggcaga gttcatgccg
    21361 ttctccggaa gcaatcttta accgttcacg catgcaccct gtgtcaatgc agtgttttaa
    21421 attgatgggg gccccattat aaccggttga taagattctc ttatccttta caatcagggc
    21481 acctacattt ctccgtatgc aggtagaacg cctcgaaaca atccgggcta tatccatgaa
    21541 gtaagaatcc caatccgggc gatttatctt cataggtcat aaatgcggga ataaaaggga
    21601 aaacttttgc aaagcccttt gaccctttct cgagtggctg attgcagcgc ctcatcagcc
    21661 ggtctttgaa gaacagcatc gataagatct gcaatcagat ccatctcacc ttctttcata
    21721 ccccgtgttg taactgctgg cgtacctacc cgtataccac ttgtcacatt cgggctcttt
    21781 gtgtcaaagg gtatgaggtt cttgttaatc atgatgccgc ttgcctctaa ggattcctgg
    21841 gcttcttttc ctgtaagacc cttatctgtg aggtcaacca taaaaagatg attgtccgtt
    21901 ccaccggaga cgattctgta accctttttc tccaaggcgc tgcacaggcg ctgagcgttt
    21961 tttatgatct gttgttgata atccttgaag ctgtctgtca ttgcctgtcc aagggcaaca
    22021 gcttttgcag caatgacgtg catgaggggg cctccctgga taccgggaaa aactgctgaa
    22081 tctatggccc ttgcaaactc ttttttacac aggatcatcc ctcccctggg gcctcgcagg
    22141 gttttgtgag tcgtggttgt aacaaaatgg gagtactcga cagggtcggg gtgaaagcgg
    22201 gcagcaacga ggccagcaat atgagccata tctactgtca gataggcacc aacttcatct
    22261 gcaatctctc tgaatttctt aaaatcaatg agcctcgggt atgcgcttgc accagcaatg
    22321 atcatctttg gtttttcagc aagagcaatc cttcgtatct catcataatc gagcatctca
    22381 ttgtcttttc ttacgtggta tccaacagcc tgataaaatt ttcctgaaaa gctgacacgg
    22441 gcgccatggg tcaggtgccc gccatgggca atgtccatac caag
//
LOCUS       DAOOMC010000033        22024 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdus sp. isolate AMR_MDS_2685
            NODE_2299_length_22024_cov_5.430197, whole genome shotgun sequence.
ACCESSION   DAOOMC010000033 DAOOMC010000000
VERSION     DAOOMC010000033.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864760
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdus sp. (wastewater metagenome)
  ORGANISM  Syntrophorhabdus sp.
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae; Syntrophorhabdus.
REFERENCE   1  (bases 1 to 22024)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22024)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,729
            Genes (coding)                    :: 2,708
            CDSs (with protein)               :: 2,708
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 7 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22024
                     /organism="Syntrophorhabdus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2299_length_22024_cov_5.430197"
                     /isolate="AMR_MDS_2685"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2699756"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(81..641)
                     /locus_tag="PK125_04915"
     CDS             complement(81..641)
                     /locus_tag="PK125_04915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB37483.1"
                     /translation="MVEARSIARKVFHFSSASIPLCYLFIGRMAALVFSLVLLALSAC
                     FEILRIGGRLNISFVTKYMEVKESESRKPTGSFYYLLAIPVTILVFQKSVAVSSLFIA
                     AIADPLCSLAGLKWGKTKIFEKSLEGSSVFFVASLLILSFFPFPTHVRLITAFVATLI
                     ELFTPKWLDDNLTIPIGTALVLTFLT"
     gene            1072..1824
                     /locus_tag="PK125_04920"
     CDS             1072..1824
                     /locus_tag="PK125_04920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB37484.1"
                     /translation="MKKLLAGTVLLGIVCFAPLPTVAQIYDDVPLAPPTNAPPPYDFE
                     APPDMVPLPYADDVYVAPYVDIDLFFWNGLWWRLWQDRWYSSYYYDRGWVYYASVPRF
                     YYNLGPGWRRHYRNYAWYRNPWYYNRTYRHRPLRNWQGWHNDNHRERVQTWSSHTYKS
                     RTRFPTKEPRLQTFQRYHPGMESQYYQQKWQHQQRQPQLRRNYQTGVERQRISGVQQP
                     RFQIQQPRLRNQAGQQMRERPRIQQRMYSRPQ"
     gene            2311..3009
                     /locus_tag="PK125_04925"
     CDS             2311..3009
                     /locus_tag="PK125_04925"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HPB37485.1"
                     /translation="MDLFSTIADSLSQTDFLSGIDAKFHSSIFHRGDRIDVQPSETVF
                     FQGDPARKCYFLLVGLIKLTKFHEQGKAVVVRFIKPGELTAAIAVFKESLYPVTAEAV
                     SHSELVGWDKQGMVALMAEYPQLAVNMLGVAFDRLQDLQARYLELFAEQVEQRIARAL
                     LRIMRQSGRKTNEGILIDFPLSRQGLADYTGTTVYTVSRVLSAWEKKGWVISRRERIT
                     VSDPHALVLFTENR"
     gene            3091..3330
                     /locus_tag="PK125_04930"
     CDS             3091..3330
                     /locus_tag="PK125_04930"
                     /inference="COORDINATES: protein motif:HMM:TIGR03980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1858 domain-containing protein"
                     /protein_id="HPB37486.1"
                     /translation="MTLDPHMTIKELLEHHPISIKVFIKRKMLCVGCPTESYHTLEDV
                     AKIYGVALGTLMESLQEAIQTTMHGSTSVKFTERS"
     gene            3337..4056
                     /locus_tag="PK125_04935"
     CDS             3337..4056
                     /locus_tag="PK125_04935"
                     /inference="COORDINATES: protein motif:HMM:NF021520.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2249 domain-containing protein"
                     /protein_id="HPB37487.1"
                     /translation="MIQDWENKKESFDVMDVRKLTGNFLPGLLTKAGKLEVGEGMCVV
                     QTFEPVPLYSAMADLGFEHLTEQVSDSEYRVYFYRTEKKEASFTGVGDMPLKPTAVLN
                     FKKIDNRLADIIVNFWSLIWGKESPAIDQKTKLLLSLANGVGAGRFRQATRELVKAYA
                     LGVTVAELDELFSMFVWNGGVGNFASEIGPSPLFGAYQLIKNLENKGISRSDIMAELL
                     DKFGESNPEVNVMPQDKGRTA"
     gene            4095..5273
                     /locus_tag="PK125_04940"
     CDS             4095..5273
                     /locus_tag="PK125_04940"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FprA family A-type flavoprotein"
                     /protein_id="HPB37488.1"
                     /translation="MLARKIEDGIYWMGAVDWDRRLFDSLIPLPDGTTYNAYLLAGSQ
                     KTALLDTVDPPMVDTLMMQLDAVPKIDYIVSHHAEQDHSGSIPRVLEKYPQAKVVVTP
                     KAKTMLADLLKLPENAFLTVADGETISLGNMTLKFIHTPWVHWPETMVTYLEERHILF
                     SCDFFGSHIATSDLFVTDKGRVHEAAKRYFAEIMMPFRSIIEKNIEKLKAYDIKMIAP
                     SHGQIYAQPSWIIDAYGEWVAGPPRNTVALPYVSMHSSTKLMVDHLASALVKNGVRVE
                     LFNLTVTDIGKLAMALVDAATIVVGTPTVLAGPHPLAAYAAFLANALRPTTKFLSIIG
                     SYGWGGKTVETLAGMIPNLKVEVLEPVLCKGLPSQDDFAALERLAESITKKHSELNLV
                     "
     gene            5367..7013
                     /gene="hcp"
                     /locus_tag="PK125_04945"
                     /gene_synonym="priS"
                     /pseudo
     CDS             5367..7013
                     /gene="hcp"
                     /locus_tag="PK125_04945"
                     /gene_synonym="priS"
                     /inference="COORDINATES: protein motif:HMM:NF015050.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016661 - oxidoreductase activity, acting
                     on other nitrogenous compounds as donors [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylamine reductase"
     gene            complement(7510..8742)
                     /locus_tag="PK125_04950"
     CDS             complement(7510..8742)
                     /locus_tag="PK125_04950"
                     /inference="COORDINATES: protein motif:HMM:NF017828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacyl-CoA dehydratase family protein"
                     /protein_id="HPB37489.1"
                     /translation="MSEKLTSRQLSKKITEEYMEEAFHAHEKGKLIGYSTAISPVELF
                     VAHDIIPIYPENHSVANLTAKKGVELCSVVEGMGYTSHLCAYARSDFGYRKTGLTVTK
                     GIPEPDLFLACNAQCFTLTKWFQVLSREGGAPVFVFDTPEYVMDKKAREEIVKYCVLQ
                     LKELISFLEEVTKKKFDYDRLKEVMKYSAQSSILYRKFLDMAQYKPSPISIFDALIGM
                     AIAVYRRGTQECVDYYQTLCDEIQAKVDQGIGVLPKDKEKYRLYWENLPVWFKFSDHA
                     KLLGSYGGVILTSLYVHAWSFEFDLDKDPLLTLAENYVSRFSNSTIEDRADMAMDLFK
                     KYDMNGMIMFMNRSCKAVSFAVPTLKDILTKRTGIPALVFESDMGDQRFYAESQVRTR
                     IEAYFETLDRLQASKSVA"
     gene            complement(8739..9944)
                     /locus_tag="PK125_04955"
     CDS             complement(8739..9944)
                     /locus_tag="PK125_04955"
                     /inference="COORDINATES: protein motif:HMM:NF017828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacyl-CoA dehydratase family protein"
                     /protein_id="HPB37490.1"
                     /translation="MWDNLSLKEVVEYANDPYPSIREWAKEKGKKVVGSTIADVPEEI
                     VHAFGFLPVTLIGTQKPLKKAPAKLPDNACSLARSNLELVMSYEGDLFDGFVLPQVCD
                     TTQHLSDIWRINFPDKYVESFLAPRQGDRPSAWYWVREEIKRLIASLSEWTGKTLKDE
                     DLQESIKVFNENKALLKEIYEIKQKNPAAITNKEYFALIKLSMQVDKAELNKSIKKIK
                     DSLDTKTKAEGYTDVIMVGITCDPPEIFDLFDEIKINIAGDTLVTGGRYLQGMVPEKG
                     GPNEDLMSVFGPSPHVELFTQNGGPVDALTARHFRRGFFSPIHDDVLRNFEEVKALYK
                     ATNAKAVVYIHIEFCESEEYDLPDLKNMIRKEGIPMHVVDTEYQTTSLSHLRTRLQAF
                     FESLKGGPL"
     gene            complement(10807..12510)
                     /locus_tag="PK125_04960"
     CDS             complement(10807..12510)
                     /locus_tag="PK125_04960"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HPB37491.1"
                     /translation="MPVEGFQPYKKEDAERYIKYRWWLGLTWGDMFDKATDLYPRKEA
                     LVDDTSRFTYGELREKVDRLAIGLMRLGIKEKDFVLLQLPNWHEFIFSFYALQKIGAI
                     VVLLVARHSFSEIEYLSSLTNPVAWIGPDHYKKTDYLPILQQVIRRHKRLKHLISVRS
                     PGNNAFTPLERLIEESELTGSNLEALAARRPEPTEVSIILPTGGTTGLPKAVPRTHND
                     YIASVEYHTRAWEVTCNDVMLTAAPVSHAQAIHNCVGGAFFHCARYVLTDSTDAEDVC
                     RVIEREKVTAFPTVPALIHRIVGLENLKDFDLSSLTRIYAGGAPSSPELVRSVYEKIG
                     CKFVNALGSSEGPAAMSRLDADIDVICYTVGEKDCPYAQVKIVDQNLREVPPNKEGEL
                     ITKGPNIFNGYFKSEEENKNVFTEDGFFRTGDLAKINEDGIITITGRIKETILRGGET
                     LSAIGIERLISSHPVVADVAVIGMPDKTLGERICAYIQLREGKKLSLEELVTYLKGIG
                     ASVLQLPERLEIMESMPLTRVGKADKKALKEDIQRKLKREDSTLSFAMKLDEKKKKLS
                     K"
     gene            complement(12501..13487)
                     /locus_tag="PK125_04965"
     CDS             complement(12501..13487)
                     /locus_tag="PK125_04965"
                     /inference="COORDINATES: protein motif:HMM:NF013134.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein subunit M"
                     /protein_id="HPB37492.1"
                     /translation="MRTFQHINAYTIEEACFLLGKNRGRAVLNAGGTDLLGILKDEIL
                     PKYPKMVINIKTIPGLDSITQNNSGVTIGALTRLSNIVNSPLIKERFPVLAEAARTVA
                     TPQIRNVATIGGNLCQDVRCWYYRYPRHIGGPIQCVRKGNGPCLAIRGDNRYHAIMGG
                     KRCFAVCPSDTAVALAALDASIHVSGPEGDRIIAVTDFFSPIANALKGNEIVTSIEIP
                     SLSHTTSQRFIKFTLRKPVDFAIVSVASIVAIEKGACIDARIALGGIGPGPVRAQIAE
                     EMVKGKAINEKNVAAAAKSALSNARPLSKNRYKIEITKTLIKRALLGDAECQ"
     gene            complement(13490..15811)
                     /locus_tag="PK125_04970"
     CDS             complement(13490..15811)
                     /locus_tag="PK125_04970"
                     /inference="COORDINATES: protein motif:HMM:NF014758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HPB37493.1"
                     /translation="MDNKYRFIGKRTPRLDAPEIVTGKAKFLNDIKLADMLYGKVLRS
                     PYAHAVIKSIDTGKAEAVKGVKAVLTWQDVPDWKGGTPRFTRILDRKVRYVGDAVALV
                     AATTEEIAKEAMHLIKVEYEVLKPVFDVEEALQPDAPQLYDEFPGNVVTPGVPFFGPK
                     NLKEVVMGDTEKGFKEADVITEGTFGYENIPNPLPPEPPGAIALWEEPNRLTLWVSNQ
                     ASYMDKITLFHVMGREVEVRSIGGPCGGSFGSKFMSWQVQTYAALLSRATGKPVKIVF
                     TKEEHLAAFTVRPSSRMHARVGMKKDGTVTAVSGKWLIDTGYYSMTTQSQIAVGCGEV
                     QIMVRCPNWDLRPVIACTNRNASGIVRGFGGQELKCILIPLLSLAMEKLNIDPFEFLK
                     KNFVKPGDGYFWRDGLWYTYRGVDFSPSMDKGAEAFGWKDKWKGWLTPTSVKGSKRRG
                     VGVGVHGNADIGEDSSEAYVRLHPDGTAMLFSCVTEHGTGQRSNYAKMVAEVLQIPLE
                     NVSITPSDSLINPYEFGPAGSRGTYAIGSAVIRAAEDAKKKLLDLMAPMFQVKPEDLD
                     TSDGVVFVREDPGKKISWKAMSIDRTITGFGRFEPDYTLTNCMMSFVEVEVDTETGKV
                     DLIQVVNATDAGKIIDPQGIEGQLNGCLGSAGIDSALFEETILDRSSGHILNVNLIDY
                     KWRTFAELPAIHSVVLETPFPSHRFHAVGIGEVATSPGPSAVLMAVSNAIGLWLHEYP
                     VTPEKVLMALGKTGSNRLNRSNSLNCSNGIHGG"
     gene            complement(15817..16353)
                     /locus_tag="PK125_04975"
     CDS             complement(15817..16353)
                     /locus_tag="PK125_04975"
                     /inference="COORDINATES: protein motif:HMM:NF013923.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HPB37494.1"
                     /translation="MTLKTGRTSKRKLSKVRSISFTVNGHDYTMKIGKEANQVDPAHT
                     LSHTLRETLGLTGTKISCDHGACGACTVIMDGKAVLSCMTLTVECHGKTITTIEGLGD
                     SETGTIDPLQQSFIDHTAFQCGFCTPGMIMSAKAILNENPTPTEDEVKEGLSGNFCRC
                     ISHYHVVEAVLAASKRVG"
     gene            complement(16616..17407)
                     /locus_tag="PK125_04980"
     CDS             complement(16616..17407)
                     /locus_tag="PK125_04980"
                     /inference="COORDINATES: protein motif:HMM:NF013757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HPB37495.1"
                     /translation="MTGDDRETFYNKSLERALQIMNAFRADRQELTIGQLAEILHLSR
                     ATVTRLCTTLVKYDYLKKNPESKKYSLGIRLFDLGSIVFDSFSLRRVASPHLSRLQMK
                     VGKTIYLGVMDNDELLYIDKREDPRNPVSFTSRVGTRRPPYWGMLGIVLMAYLAVSEV
                     ERLLQKYPLAATTKKSITGNEEYIERLSKVRKQGYYVEIEEVFEGIGGVSAPVRDFSG
                     KVIAGIGVGFISSSVDSKGLKRIIKEVTETAGVISREMGYVSRGS"
     gene            18026..18442
                     /locus_tag="PK125_04985"
     CDS             18026..18442
                     /locus_tag="PK125_04985"
                     /inference="COORDINATES: protein motif:HMM:TIGR00149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HPB37496.1"
                     /translation="MKSYRKELWFNVPGRRGFVNITPQIEDCIRESGITEGITLINAM
                     HITASVFINDDESGLHHDLEVWLEKLAPHEPVSQYRHNVGEDNADAHLKRSVMGREAV
                     VAITDGKLDFGPWEQIFYGEFDGRRRKRVLVKIIGD"
     gene            18779..18961
                     /locus_tag="PK125_04990"
                     /pseudo
     CDS             18779..18961
                     /locus_tag="PK125_04990"
                     /inference="COORDINATES: protein motif:HMM:NF012675.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin family protein"
     gene            18978..19889
                     /locus_tag="PK125_04995"
     CDS             18978..19889
                     /locus_tag="PK125_04995"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HPB37497.1"
                     /translation="MYDVMIIGAGPSGMTAAVYAARKKLHTLLISKDIGGQTNWTNSI
                     ENYMGYQFIEGPELIEKFENQVKQFPIDLKIGDGVSSISLIDKGFEIRTESGQTYQGK
                     TGIIATGKRPRSLNIPGEEKFRGKGVSYCSICDAPLFEGMNVAVVGGGNSALEAADDL
                     IKIGEHVYLISLTPLTGDQVLIDKVTKAPNLTVYTEHETLSIDGDNFVSRITVRDLKS
                     NEIRELDVKGIFVEIGLIPNSDLAKGLAPFNRLSEIEVNCNCETVVPGLFSAGDVTNT
                     PEKQIIIAAGDGAKAALRVYHYLQRLS"
     gene            complement(20061..21494)
                     /locus_tag="PK125_05000"
     CDS             complement(20061..21494)
                     /locus_tag="PK125_05000"
                     /inference="COORDINATES: protein motif:HMM:TIGR04315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetrathionate reductase family octaheme c-type
                     cytochrome"
                     /protein_id="HPB37498.1"
                     /translation="MKIQRFMIAVTAFAVGALVSVVWANHADLVGEGPFKTGPDVTRK
                     CLECHEKETKDFMKTVHWTWSKEQTLESGKKVNIGKVNALNNFCIGLPGNWPRCTSCH
                     AGYGFKDAKFDFSKQENVDCLVCHDTTGMYKKFPTGSGHPVYDGEQKEFPKGNVWKPV
                     DLLKVAQSVGRTSRATCGSCHFYGGGGDHVKHGDLDTSLANPTAEIDVHMGGPKKMTC
                     QDCHQAKDHFLKGEAASVTITHTEPSHIDCIDCHKGRVHKNALVNKHTEKVACQSCHI
                     PTFAKDRPTKVWWDWSSAGKDVKPEDIPKDKYGQKTYDKMKGDFKWEKNVIPTYMWFD
                     GNIVRYLAGDKIDPTKIVQLTSVKGSRNDPKARIFPFKIMRGKQAYDSGNNTIAFTQV
                     FGPPGSDAYWQKYDWNLAITSGMKAAGLPYSGKFGFIETSMVWPVHHMVVPKDKSLKC
                     IDCHSDKGRLDWKALGYKGDPKDPKNR"
     gene            complement(21813..>22024)
                     /locus_tag="PK125_05005"
     CDS             complement(21813..>22024)
                     /locus_tag="PK125_05005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB37499.1"
                     /translation="QYPALRAICNSLLPAGDACGWFGCRITKPATVFIIEFQSMSIWM
                     HHCYKLICSFVVKHLFHRMNADERR"
BASE COUNT         5851 a         5173 c         4986 g         6014 t
ORIGIN      
        1 ccagttactg agacggaatt gaggtaaaaa cttgtctgga cttatggtac aggtgtgtgg
       61 agtgtaggcg tggttttgga ttaagtcaaa aaagtcagca caagggctgt accaatggga
      121 attgtgaggt tgtcatcaag ccacttcggg gtgaagagct ctataagggt tgcaacaaat
      181 gcagttatca gacggacatg ggtgggaaag gggaaaaagg atagtatcag aagagaagct
      241 acaaaaaata cagaacttcc ctcaaggctt ttttcaaaga tctttgtctt accccacttt
      301 aagcctgcaa gagaacaaag gggatctgca atggctgcta tgaaaaggga ggaaacagcc
      361 actgattttt gaaaaacaag aatggtgaca gggatagcaa ggagatagta aaaactccct
      421 gtaggttttc tggattcaga ctctttcacc tccatgtatt ttgtcacaaa ggagatattg
      481 agtctccctc ctatacgcaa aatctcaaaa caagccgaca gggcaagcaa gaccagtgaa
      541 aagactagtg cagccatcct acctataaaa agatagcata gaggtatgga tgcactggaa
      601 aaatgaaaaa cctttctggc tatcgaccgt gcttcaacca ttcctttaca tcccatgtca
      661 tattttttgt attattgtca gttttgcact cgcagctttt atactgtatc gaccaggaga
      721 aatcaaatct ctcttttcat gaatgggatg catagggttg tggtcttcag agatacggtg
      781 tttgatgctc atgtgttatc ctggtagatg aacatcgata ctaaacctct ttaatccctt
      841 tatatatttc aggcccttca ggttgatggc agattctttg tcgacagttt cgtcggaaat
      901 tcctatagta aacgacgaaa atgttggcca ttcagcgctt gataaaataa ttaccaataa
      961 tatcaagata gttagagtac gcatgtttgg gtatcaatat tgctcattac cgtgtaaagc
     1021 cagacacagt cttaaacaat gttgctggcg atggagaaag gaggcagtgc gatgaagaaa
     1081 ttactggcag gaacagtgct tttggggatc gtctgttttg ctcctcttcc aactgtggca
     1141 caaatatacg atgatgttcc tcttgccccg ccaactaatg caccaccacc ttatgacttc
     1201 gaggcaccac ctgatatggt gccattaccc tatgcagacg atgtttatgt tgctccttac
     1261 gtggatatag atctcttctt ctggaacgga ttgtggtggc gcctatggca ggaccggtgg
     1321 tatagctcat actattatga tcgtggctgg gtgtattatg cttctgttcc aagattctat
     1381 tataacctgg gccccggatg gagaagacac tacagaaatt acgcatggta cagaaatccc
     1441 tggtattata atagaacgta cagacaccgg cctttacgga actggcaggg ctggcataat
     1501 gacaaccaca gagaaagagt gcaaacatgg agcagtcata cgtacaaatc caggacacga
     1561 ttccccacga aagaaccaag gctgcagact tttcagaggt atcatccggg aatggaatcc
     1621 caatattatc aacaaaagtg gcaacatcaa caaaggcagc ctcagctcag acggaactat
     1681 cagacagggg ttgaaaggca gcgcatctct ggtgttcaac aaccacggtt tcagattcaa
     1741 cagccgaggc ttcgcaatca ggcgggacaa cagatgagag aaagaccaag gattcagcaa
     1801 cggatgtact cccggcctca atgacagctc tcagccaaag gacatagaaa agggcggcgg
     1861 ccctctgcga gcatagtaac catgaggaga agtctctgcc cgctttgccg gtcatcttcc
     1921 tgtgctgtga agagcaacga actgatttcc aaatccctgg taaaggtaaa tacaagccac
     1981 tataatatat ttttatatgg attacagact ctttcaaaaa gagaatacgt ggtagtttaa
     2041 ataaaaaatt gaaaagggac agcataaggt atggtactgt ttctgacaaa agaagaagga
     2101 tcacttagac aataccatcg tctatgtgac cctggttcct aaaactttac atcgcttttt
     2161 atttcctagt gttacctagg gatcttcttg ctgtgatgga aaaaacaact ttaaagatgg
     2221 tataattgta cgatggcaga taaatgaatg taagagattc tttccgcacg cttttgaaga
     2281 ggtatttcaa aagttttgaa ggatgttttc ttggatctat tttctacaat agccgacagt
     2341 ttaagccaga ctgatttcct atcgggcata gatgcaaaat ttcacagtag tattttccac
     2401 cgtggagacc gaatcgatgt gcagcccagt gaaacagttt ttttccaggg tgatcctgcc
     2461 agaaaatgct attttctttt ggtgggcctc ataaagctca caaaattcca tgaacagggt
     2521 aaagcggttg ttgttcgttt catcaaacca ggagaattga cagccgcaat agccgttttt
     2581 aaagagagtc tttatcctgt caccgctgaa gcagttagtc attctgaatt agtcggttgg
     2641 gacaagcagg gtatggtagc cctcatggca gagtacccac aattagcagt caacatgctc
     2701 ggagttgctt tcgatcgact tcaggatttg caggccaggt acctggagct cttcgccgaa
     2761 caggtagaac agcgcatcgc ccgtgctcta ttgcgcatta tgcgacagtc cggtcgtaaa
     2821 accaatgagg gcatcttaat cgacttcccg ctcagccgtc agggactggc agactataca
     2881 ggcaccactg tatacactgt tagccgggtt ctcagtgcct gggagaagaa gggttgggta
     2941 atttccaggc gagaacgtat cacggtgtct gatccccatg ctctggtcct gttcacagaa
     3001 aaccgataat taggaaagat caatatattc tttcgatcat tgcgcctgcg caatgatgag
     3061 gtagagcaaa agtattatcc tgatacaaca atgactttag acccacacat gacgatcaag
     3121 gagttgctgg agcatcatcc gatatcaatc aaggttttta tcaaacggaa gatgctttgc
     3181 gtaggatgcc cgacagaaag ctatcacaca cttgaagatg tagccaaaat ttacggggtt
     3241 gccctgggca cccttatgga aagtttgcag gaagctatcc agacaacaat gcacgggtcg
     3301 acatcagtca aatttacaga aaggagttga gaaaagatga tacaggattg ggaaaataaa
     3361 aaagagagtt ttgacgttat ggatgtgaga aaattaaccg gtaatttttt gccggggctt
     3421 ctcacaaaag caggaaaact tgaagtaggt gaaggtatgt gtgtggttca aacttttgag
     3481 cctgttccgc tttattccgc catggcagac cttggcttcg aacatcttac tgagcaggtt
     3541 tccgacagtg aatatcgggt ctatttttac aggactgaga aaaaagaggc ttcctttact
     3601 ggtgttgggg atatgccttt gaaaccgacc gccgttctga attttaagaa aattgataat
     3661 agattggcag atatcatcgt gaacttctgg agcctcatat ggggaaaaga gagcccggca
     3721 attgatcaga aaacaaaatt gttgttatct ctggcaaatg gtgttggtgc agggagattc
     3781 cgacaggcaa cacgagaact ggtgaaagcc tatgcattgg gagtcaccgt agcagagttg
     3841 gatgagctct tttccatgtt tgtttggaat ggcggtgttg ggaactttgc ttccgagata
     3901 gggccttccc ctcttttcgg ggcctaccag ctcattaaaa atcttgaaaa taaaggtata
     3961 tcccgctcag atataatggc agaacttttg gataagtttg gagagtcaaa tcctgaggta
     4021 aacgtaatgc ctcaagacaa aggcaggaca gcataggcct gggcagtgaa tataacgagc
     4081 ataaggaggg tacgatgtta gcacgaaaaa tagaagatgg tatttattgg atgggggctg
     4141 ttgactggga cagacgcctc ttcgattccc tcatccctct tcctgatggt accacgtata
     4201 atgcatatct gcttgcgggg agtcagaaga ctgctttact cgacacggtt gacccaccga
     4261 tggttgacac cttgatgatg caacttgatg cagtgccgaa gattgactat attgtctccc
     4321 atcatgccga acaggatcat tccggatcta tcccccgtgt tctcgaaaag tatcctcagg
     4381 caaaggtggt tgtaacacca aaggccaaga caatgcttgc agatcttctt aaattacctg
     4441 agaacgcctt tctcaccgta gccgatgggg agacgatatc cctcggaaat atgacgttga
     4501 aattcattca cacaccctgg gttcactggc cggaaacgat ggtaacgtat ctggaggaaa
     4561 gacatatcct ctttagctgc gatttttttg gttctcatat cgccacaagc gatcttttcg
     4621 tcactgacaa gggacgggtg catgaggcag cgaaacgtta tttcgccgag attatgatgc
     4681 ctttccggag tattattgag aaaaatatcg agaagttaaa ggcttacgat ataaaaatga
     4741 ttgcaccaag tcacgggcag atttacgcac agccatcatg gatcattgat gcctatggag
     4801 aatgggtcgc aggtcctcct cggaacacag tcgctttacc ctatgtgtcc atgcattcga
     4861 gtacaaagct gatggttgac catctggctt ctgcactggt aaaaaatggt gttcgggttg
     4921 aactgtttaa cctgaccgtg accgatatcg gtaaattggc catggctctc gtagatgctg
     4981 caacgatagt tgtcggcaca cctacagtgc tggcaggccc ccatcctctg gcagcatacg
     5041 ctgcatttct ggcaaatgcc ctaaggccaa caacaaaatt cctttccatc ataggatcct
     5101 atggatgggg tggaaaaaca gtggaaacac tggcaggtat gattcccaac ctcaaagttg
     5161 aagtcctgga gccggtgctt tgcaagggac tgccgtctca ggatgatttt gccgcactgg
     5221 aaagattggc cgagtcaatt accaaaaagc actcggaatt aaacctggtt tgacaatgta
     5281 ctatgattcc ttttcattta ctcatgtgct tttcattttt caaggttggt atgtttttat
     5341 aaaccacaat aaataggagg gacactatgt tttgttttca atgtcaggaa accgcaaaaa
     5401 acacaggctg taccgtaaaa ggcgtatgcg gcaagcccga agtaactgct aatctccagg
     5461 atctgctcat ctatatatgt aaaggaatag ccatctacgg tgaaaaagca aaggaaagcg
     5521 gtgttttaga taaaaaagct gcccgtttca tttgcaaggc actttttacc actattacca
     5581 atgtggcctg ggatgatgat gtcataattg aacgtatcag ggaagcactc aaagttcgcg
     5641 atgagatcag agagaaggcc ggggctgcca ttgccggctc acttccagat tgtgctacct
     5701 gggcatccaa tgacaaggaa aagattgtgg ccaaggcgat gtccgatgag gtgcgcatta
     5761 ccgctgccca gaacgaagat gttcgctctc tgcgagaact actcattatc ggctgtaaag
     5821 gaatagctgc ttatgctgat cacgcggcag tccttggcca tgagacggat gatatctatg
     5881 gtttcctcat ggaggccctt gcatccacta ccaaagacct ttcaatggat cagatgattg
     5941 ccatggttat gaaggccggg gaaaccgctg taaatactat ggcacttctc gataaagcca
     6001 acacatcagc gtacggaaat cctgagatca cggaagtcaa catcggagtt ggaaaaaatc
     6061 ctggaattct catatcagga cacgacctga aagacatgga ggaactcctc aagcaaacgc
     6121 agggaacagg tgttgatgta tacacccatg gagaaatgct gcctgccaac tactatccgg
     6181 ctttcaaaaa gtataccaat tttgtcggta attacggtgg ttcctggtgg catcaaaacg
     6241 atgaatttga gtctttcaat ggtcccatca tacttaccac caattgcttg atccccataa
     6301 aaaaggataa ttcatacttg gaaaggcttt ttacaacggg cgtggtcaac tatcccgggg
     6361 cgaaacatat accagagaga cctgaaggtg gtgccaaaga tttctcttct gttgtggcac
     6421 tcgcaaaaac atgtcagtca ccgaaagaaa tcgagtcagg gaaaattatc ggtgggtttg
     6481 cccataacca ggttttggct ctcgcagata aagtcataga tgcagttaaa tcaggagcta
     6541 ttcagcgctt tgtcgtaatg gcaggatgcg atggtcgtca aaaaacccgg ggttatttta
     6601 ccgaagtagc cgaaaacctg ccaagaaaca ccgtaatcct tacggctgga tgtgctaaat
     6661 accgttataa taagctagcc ctgggcgaca tcggaggcat cccccgcgta cttgatgcag
     6721 ggcaatgtaa tgattcttac tctctggccg tgatcgccct taagctcaaa gaagtctttg
     6781 gactgaaaga cattaatgaa cttcccatat catatgacat tgcctgatat gaacaaaaag
     6841 cagtggccgt attgcttgca ttgctctacc tcggtgtcaa gggtatccgc ttagggccta
     6901 ctctgccagc gttcctttca cccaacgtag ctaaggtcct ggtggagaaa tttaatatca
     6961 aacctatcgg tagtgtacag gaagatatag ctgcaatgat gaagggagaa tagcataact
     7021 catccatgga gtcacacagc gatataacca cctgatatac ttgcacggaa accatcacgg
     7081 cccctctttc ccttgaaggt ttccgtgctc ctccaaacaa taaaattgca tacactggct
     7141 aagtggaaat ccttcttgtg tgtcttgtag aaaggcttac agatgtagcc aagaacatga
     7201 agaaaaagta ttgtgacagg tttgaactaa aacgatttat accaaggata tgaaggaatc
     7261 aggtcattct tttctgaaca aaacgtcgag agatggcgat agtagaaact gagaagttaa
     7321 gaatagcatc atattgactt ttctcatctt cttgattttc atattcttag cttctttttt
     7381 gaggggaaaa gcctatctgc tcctcatcca tccgggaaat tcgtagtaat aaattgaagt
     7441 ctgaatttca ttaaaaaccc cggatgtttt catccggggt cctggtttgc actgtttgtg
     7501 tttgtgcaac tatgcaactg attttgacgc ctggagcctg tccagcgttt caaagtaggc
     7561 ttcgatacgg gtcctcacct gcgactctgc atagaaccgc tggtcaccca tgtcgctctc
     7621 aaatacaaga gcaggaattc cggttctctt tgtgaggata tctttcaggg taggcactgc
     7681 aaatgacact gccttgcaac tcctattcat aaacatgatc ataccgttca tatcgtactt
     7741 cttaaagagg tccatggcca tatcagcacg gtcctcgatg gtagaattgg aaaatctcga
     7801 aacatagttt tccgccaggg tcaaaagcgg atctttgtca agatcaaact caaagctcca
     7861 ggcgtgaaca tagagagatg tgagaattac cccgccgtaa gaacccagga gttttgcgtg
     7921 atcactaaat ttgaaccaca ccgggagatt ttcccagtac agacgatact tttctttgtc
     7981 tttcggaagt acgccaattc cctgatccac tttagcctga atctcatcgc agagagtctg
     8041 atagtaatct acgcattcct gtgtgcccct tctgtacaca gcgatagcca taccgatgag
     8101 tgcatcaaaa atactgatgg gagacggctt gtactgtgcc atatcgagga actttctgta
     8161 aagaatgctc gactgggccg aatatttcat gacctccttc agccggtcat aatcaaattt
     8221 cttctttgtc acttcttcaa ggaaactgat cagctctttg agctgaagga cgcagtattt
     8281 aacgatttct tctcgcgcct tcttgtccat cacatattca ggtgtgtcaa aaacaaaaac
     8341 aggcgcaccg ccttctcttg agagaacctg gaaccatttt gttagggtga agcactgggc
     8401 attgcaggca aggaacaagt ctggttcggg tattcccttg gtgactgtta aacctgtctt
     8461 tcgatagcca aagtcgcttc gcgcataagc gcagaggtgg cttgtatatc ccataccttc
     8521 aacgaccgag cacagctcaa cgcctttctt tgcagtcagg tttgctacgg agtggttttc
     8581 aggataaatg ggaataatat catgggcaac gaagagttcc actggtgaaa tagctgtgga
     8641 gtagccgata agttttccct tttcgtgggc atggaaggcc tcttccatgt attcctcggt
     8701 aatcttcttt gataactgtc tcgaagttaa tttttcgctc ataggggacc ccctttcaag
     8761 gattcaaaaa atgcctgcaa tcttgttctc aggtgggaaa gtgatgtggt ctggtactcg
     8821 gtatcaacca catgcatcgg tattccttcc tttcgaatca tatttttgag gtcaggcaga
     8881 tcgtattctt cagattcgca gaattcaata tgaatataaa cgactgcctt tgcgttggtt
     8941 gccttgtaca gggctttcac ttcctcaaaa tttcttaaaa catcgtcatg gataggtgag
     9001 aaaaaccctc tcctgaaatg cctggctgta agcgcatcta ctggccctcc attttgagta
     9061 aacaattcaa catgtggtga cgggccaaaa acactcataa ggtcttcatt gggtccccct
     9121 ttctcgggta ccatgccctg aagataacga ccgccagtta cgagagtatc ccctgcgata
     9181 tttatcttga tctcatcgaa gagatcgaat atttctggtg gatcgcatgt gatgcccacc
     9241 attattacat cggtgtaccc ttcagccttt gtttttgtat caaggctatc tttaattttc
     9301 ttgatactct tgttcagctc tgccttatct acttgcatgg atagtttaat cagggcaaaa
     9361 tactctttgt tcgtgatagc agccggattc ttctgtttga tttcataaat ttctttcaga
     9421 agggccttgt tttcattaaa tacttttatt gattcctgca agtcttcatc tttgagagtc
     9481 ttgcctgtcc attccgagag ggatgcaatc aacctcttga tttcctctct gacccagtac
     9541 caggcgctcg gacggtcgcc ctggcgcggt gcaagaaaac tttcaacata tttgtcaggg
     9601 aagttgatcc gccagatgtc tgacaggtgc tgtgttgtgt cgcacacctg gggcaagaca
     9661 aatccatcga aaagatcgcc ctcgtaactc ataaccaatt caaggttact ccgagccaga
     9721 gagcatgcat tgtctggcaa ctttgctggc gccttcttta gaggcttctg cgtgcctatc
     9781 agcgttactg gtaaaaaacc aaaagcatga acgatctcct caggtacgtc agcaattgtg
     9841 ctgcccacaa ccttctttcc cttttctttc gcccattctc ttatggacgg atagggatcg
     9901 ttcgcatact cgaccacttc cttaaggctt aggttgtccc acataagtcc tccacattgt
     9961 ataggaaaaa tttcacatat tatagaaagc ccttactttt ttgtcaacaa tagaatacac
    10021 tgtaaatctt tttagtgaat aaatagggaa aaattagctg gaaatcacaa aagtatcagg
    10081 tattccatga agcaaacagg caagccgagg aggggctaat ttgagaaaag ccccaaagta
    10141 aataatggct gcccttcaga tcgtatggcc agaacacggc cagaccgtgt aagacaaaga
    10201 accgtaaaca ccatggtctg ccagtgtgaa cctgaagttg taatagacca tgattgaatt
    10261 gacagggtga caactgccaa gagaatgcct gcccgggtag tgcgataacc aggccagggg
    10321 caaaaacact ttccttcggt tacttcgcaa gaaggatcat gtgagctgcc actttccata
    10381 tatccgttcc ggtgatgccc acaacaaaac cgggggatgc cagatatgat ggaggcactg
    10441 ccaatctcct tataaaaaac gagacttaat atgctttatc ccttattcga actcaagctt
    10501 tttgttgcat tgctaacctg taagcattcg tatagtgttc aacccgctct tttccgaagg
    10561 caagctatgg gtcggcttcc tcccctgtaa aacctttgct taccgtatct ttcttccatt
    10621 cagcaattac aggctgagat gcctgcggcc actgcacagt cgcgttgtca gagatgagaa
    10681 aagcttatca gtccacttgc ccttgatctc attgcctgct tcctgaaaga tcttcctaat
    10741 atcagtggat aggtgatccc ttttggcctt gttcataacc tcacttgcaa tcccccttga
    10801 tctgattcac ttgcttaact ttttcttctt ttcatccaac ttcattgcaa aagacaaagt
    10861 actgtcttcc ctcttcagct ttctctggat atcttctttt agcgcttttt tgtctgcctt
    10921 gcctaccctg gtaaggggca tgctttccat tatctcgagc cgttcaggca actgaagaac
    10981 tgaggccccg atgcctttga gataggttac cagctcttca agggaaagtt ttttcccttc
    11041 cctgagctgg atgtatgcac atattctttc accaagggtc ttgtcaggca tgccaatgac
    11101 tgcaacgtcg gcaactaccg ggtgggaact tatcaacctt tcaattccga ttgcgctcag
    11161 cgtctcaccg ccacgaagga ttgtctcttt tatcctgcct gttatggtaa tgataccgtc
    11221 ttcattgatc tttgccaggt cgcctgtcct gaagaaccca tcctctgtaa agacattctt
    11281 gttttcttct tctgatttga agtatccatt aaagatgttc ggaccttttg ttattagttc
    11341 accttctttg ttcggcggca cctcccgcag attttgatcg acaatcttaa cctgggcgta
    11401 cgggcaatcc ttttcgccaa cggtataaca gattacatcg atgtctgcat cgagccggct
    11461 cattgctgcc gggccctcgg aagagccaag ggcattgaca aacttgcagc cgatcttttc
    11521 gtagacgctc ctgaccagtt caggagaact gggcgcacct cccgcatata ttcttgtgag
    11581 agaactgaga tcaaagtcct tcaggttctc aaggccaaca atcctgtgga taagtgccgg
    11641 tactgttgga aaggccgtga ctttctccct ttctatgacc ctgcagacat cctcagcatc
    11701 agtgctgtca gtaaggacat acctggcgca gtggaagaaa gcaccgccaa cacagttatg
    11761 gatagcctgg gcatggctta ccggagcggc agtcagcatt acatcgttgc atgttacctc
    11821 ccatgccctt gtgtgatatt cgacgctggc aatatagtca ttgtgtgtcc tgggaactgc
    11881 tttgggcaga cctgttgtcc ctcctgtggg taggattatg gaaacctctg tgggttcagg
    11941 tcgtctggca gcgagagctt cgaggtttga tcctgttaat tcactttcct caataagtct
    12001 ctccagggga gtgaaggcgt tattgcctgg ggatctcacg gatatgaggt gtttcagcct
    12061 tttgtgtctc cttatcactt gctggagtat gggcaggtag tcagttttct tataatggtc
    12121 aggcccgatc catgcaaccg gattggtgag actgctcaaa tattcaattt ccgaaaaact
    12181 gtgacgcgcc acaaggagaa caacaatagc ccctattttc tgcaaagcgt agaaggaaaa
    12241 gataaactca tgccagttcg ggagctgaag gagaacaaag tccttctcct taatcccaag
    12301 cctcataagc ccgatggcaa ggcggtccac tttttcccgc agttcgccgt aggtaaaccg
    12361 ggaagtatcg tctacaaggg cttcttttcg ggggtagaga tcagtcgctt tatcgaacat
    12421 gtccccccag gtaagcccga gccaccacct gtacttgata tatctttctg catcttcctt
    12481 cttatatggt tgaaatccct ctactggcat tcagcatctc cgagtaaggc cctcttgatg
    12541 agcgtttttg ttatttcaat cttatacctg ttcttactga gcggtctggc attcgacagg
    12601 gctgatttgg cagcggctgc aacgttcttt tcgtttatgg cctttccctt gaccatttct
    12661 tcggcaatct gtgccctgac cggtccagga cctatcccgc caagagcaat gcgggcatca
    12721 atgcatgcac ctttttcaat ggctactatc gatgctacac tcacgattgc aaaatcgacc
    12781 ggcttcctga gggtgaattt aatgaacctc tgactggtgg tatgcgaaag agaaggtatt
    12841 tcaatacttg taacaatctc gtttcctttc agggcgtttg caatgggact gaagaaatca
    12901 gtaactgcta tgattcgatc gccttcaggg cctgaaacat ggatagatgc atcaagagcc
    12961 gccagtgcaa ctgcagtatc agaagggcac acggcaaagc atctttttcc gcccataatt
    13021 gcatggtatc ggttgtcgcc tcttattgcc aggcatgggc cattcccctt gcgcacgcac
    13081 tgaatcggcc ctcctatgtg gcgcgggtag cggtaatacc agcaccgtac atcctggcag
    13141 agattcccgc caatcgtagc aacgttacga atctgaggcg ttgccacagt acgtgctgcc
    13201 tctgcgagga caggaaaccg ttcttttata agtggcgaat tcactatatt actgagcctt
    13261 gtcaacgcac cgatggtaac accgctgtta ttttgagtga tcgaatcgag gccggggatg
    13321 gttttgatat tgatgaccat ttttggatat ttaggaagaa tttcatcctt gagaatacca
    13381 aggagatctg ttccgccagc attgagaact gccctgcccc tgttcttgcc aaggaggaaa
    13441 caagcctcct caatcgtata ggcgttgatg tgctgaaacg ttctcattgt caacctccat
    13501 ggattccatt tgagcagttt aaactgtttg aacggttcaa tcggttggaa cctgtttttc
    13561 caagcgccat gagcaccttt tccggtgtca caggatattc atggagccac agccctattg
    13621 cgttggatac agccattaat acagctgaag gcccgggcga agtcgccacc tcgccaatcc
    13681 ccaccgcatg aaatcgatgg ctgggaaaag gtgtttcgag aacaacactg tggatggcag
    13741 gaagctcagc aaaggtacgc catttatagt caatcagatt gacgttcagt atatgtccgg
    13801 aagaccggtc gagtattgtc tcctcgaaaa gggcactgtc aatgccggca gatccaagac
    13861 atccattgag ttgtccctca ataccctgag ggtcaatgat ctttcctgca tctgtggcat
    13921 ttactacttg aatgagatct acctttcctg tctcagtgtc aacctcaacc tcaacaaagc
    13981 tcatcatgca attggtcagg gtatagtcag gttcgaaacg gccaaaacct gtaattgttc
    14041 tgtcaataga catcgccttc caggatattt tcttcccggg atcttccctg acaaagacga
    14101 ccccgtctga tgtatcaaga tcttcgggct tcacctggaa catgggagcc atgaggtcca
    14161 ggagcttctt ctttgcatcc tcagcggctc gtatgacggc gcttcctatg gcgtacgttc
    14221 cccgggagcc tgccggtcca aattcgtatg ggttgataag ggaatcagat ggggtgatgg
    14281 aaacattttc caaaggaatc tgcagcactt cagcaaccat ctttgcgtaa ttgctcctct
    14341 gacctgtgcc gtgttctgtt acgcaggaga agagcatggc agtcccgtcc gggtgaaggc
    14401 ggacgtatgc ttcagaagaa tcctctccaa tatctgcgtt tccatggact cctacaccaa
    14461 caccccgtcg ctttgaaccc ttgactgaag tcggtgtgag ccagcccttc cacttgtctt
    14521 tccagccgaa agcctcggcc cccttgtcca tggacggaga gaagtcaact ccgcgatacg
    14581 tataccagag gccatcccgc cagaagtatc catcacctgg tttgacgaag tttttcttga
    14641 gaaattcgaa cgggtcgatg ttcagctttt ccatggcaag gctcagaagg ggtatgagta
    14701 tgcatttgag ttcctggccg ccgaatcccc gcactatgcc agaggcattg cgattcgtgc
    14761 aggcaattac cggtctcagg tcccagttcg ggcacctcac cataatctga acctcaccgc
    14821 agccgactgc aatctgcgac tgggtggtca ttgaatagta gcccgtgtca atgagccatt
    14881 ttcctgaaac cgctgttact gtcccgtcct tcttcattcc cactcgcgca tgcattcgtg
    14941 aagacgggcg caccgtgaat gctgcaaggt gctcctcctt ggtgaaaaca atctttacag
    15001 gtttgcctgt ggccctgctc aggagagctg catacgtctg cacctgccac gacatgaatt
    15061 ttgatccgaa gctgccaccg caaggaccgc cgatacttct cacttcgacc tccctgccca
    15121 ttacgtggaa gagagtaatc ttgtccatat aggaagcctg atttgagacc cagagggtaa
    15181 gtctgttcgg ttcttcccaa agggcaatag ctccgggcgg ctccggggga agcgggttgg
    15241 gaatattttc gtagccaaag gtaccttctg tgattacatc tgcctcttta aaccctttct
    15301 ccgtatctcc catcacaacc tctttcaaat tcttgggacc gaaaaagggc acaccaggag
    15361 taaccacatt ccccgggaat tcatcataca gttgaggagc gtcaggctgc aaagcctctt
    15421 caacatcaaa gactggcttc agcacctcgt actccacctt tatcagatgc attgcctctt
    15481 ttgcgatctc ttcagttgta gctgcaacaa gggcaacagc atcccccacg tatctcacct
    15541 tcctgtcaag gattctggta aatctgggag ttcctccctt ccagtcagga acgtcttgcc
    15601 aggtaagcac tgcctttaca cctttgactg cctcggcctt gcctgtgtct atgcttttga
    15661 tgacagcatg ggcgtaggga cttctcaata ctttgccgta cagcatatcc gccagcttga
    15721 tgtcgttgag gaactttgcc ttccctgtca caatttccgg ggcatccagc ctcggtgttc
    15781 ttttccctat gaaacggtat ttgttgtcca tctctgttac cccactctct tcgatgccgc
    15841 caatacggct tcaacaacat ggtaatgact gatgcacctg caaaagttcc ccgagagacc
    15901 ttccttcacc tcgtcttctg tgggcgtggg attctcattc aggatagcct ttgcgctcat
    15961 gatcatgccg ggggtacaaa aaccacactg gaatgcagtg tgatcaataa aagactgctg
    16021 gagcgggtcg atcgtccctg tctccgaatc tccgagcccc tcgatggtag ttattgtttt
    16081 cccgtggcac tcgacagtca aagtcataca ggaaagtact gcttttccat ccataatgac
    16141 tgtacaggcg ccgcaggcgc catggtcaca ggatatcttc gttccagtta ggcctaatgt
    16201 ttctctgagc gtatgggaca gagtgtgtgc cggatcaacc tgattagctt cctttccaat
    16261 tttcatagtg tagtcatggc cgttaacagt aaagctaatg ctccttacct tactgagttt
    16321 cctttttgat gtccttccgg tttttaaagt cataccttcc cctccagtga actatgtcca
    16381 cgatgtggtc tgcttttggg tagcacccgg tgcattgtaa tctgcctgct cgagctatac
    16441 cgttgtcctc cctcgacaaa aaacatagta cccttcctcg ttaaatgatc atttaatgat
    16501 cgttatgttc atttattgaa aattatacca ccggaaaaag ttttgtcaat caaataattt
    16561 gatggccttg atttttgtcg tcaaaggacg gatacattcg acgtatagat ttgttttatg
    16621 atcctctact cacatagccc atctctctcg aaataacccc cgcagtctca gtaacctctt
    16681 ttataatcct cttcaaaccc ttcgagtcta cagaagaaga gataaatccc acaccaatgc
    16741 ctgctatgac ctttccggaa aaatcacgta ccggagcaga cacaccgcca ataccctcaa
    16801 agacttcttc tatttcgacg taatagccct gctttcgtac cttgctgagc cgctcaatat
    16861 attcttcatt tccggtaata gatttctttg ttgtagcagc aagagggtat ttttgaagaa
    16921 gtctctctac ttcacttaca gcaagatatg ccatgagcac aattcccaac atgccccagt
    16981 atggcgggcg ccgtgtgcca acccttgatg taaaactaac agggttccgc ggatcttccc
    17041 ttttatctat atagagcaac tcgtcgttat ccattacccc caaatagata gtcttgccca
    17101 ctttcatctg caagcggctc agatgagggg atgcaacgcg ccttaaggaa aaggaatcaa
    17161 agacgatact gccgagatca aacaacctga tgccaaggga gtatttcttt gactcgggat
    17221 tttttttcag atagtcgtac ttgacgaggg ttgtacacag ccgcgtaaca gtggccctgg
    17281 acaaatgcag gatttccgca agctgtccga ttgtcagctc ctgtcggtca gcccgaaagg
    17341 cattcatgat ttgcaaagcc ctctctaaag acttgttata gaatgtctcc ctgtcgtctc
    17401 cagtcatgag ttcctctgtg aaaattgagc agatacaatc tgcaccaccc atcattgttt
    17461 gtcaacgaaa aatgtgttga ctaccaatgt gagaaagggc gctatctgga taatcttatc
    17521 tttgcctctg cctctgagta gttgatgtgg tattcaaatt cgtttttttt gcatcccttg
    17581 attgtaccct cctccttttg agccccatcg acttgtgtgc aggaataagg ctgtttcccc
    17641 gttccttaaa accgccaaac atgactcccc tgttttcatg gacaaaagcc gccgatggga
    17701 acgtacaggg gcattctgta tatcatcaca tcagctatat atgccggcag aagccaaccc
    17761 aacaagacta tgtaaccaaa agacggttat tcatcccaag gaactccatc ttatcagtca
    17821 gccatgtctg tcaataagga aaccatttgc gttatgaaca caggtagatg ggaatgtctc
    17881 aatgcaaaat cacagggcct atcctgccac agaacccaaa ggtgggtcgg gttctgactg
    17941 aaggcacgaa acaaaagatg ttcaattttt gcttcaactt attattcggt tttggtaaca
    18001 taaagcacag cggaggaaaa ccaaattgaa aagctatcga aaagaacttt ggttcaatgt
    18061 acctggcagg cgggggtttg tcaacattac acctcagatt gaagactgta tacgggaaag
    18121 cgggataacc gaaggtatta cactgataaa cgctatgcac ataacagcat ctgttttcat
    18181 caatgacgat gaatcagggc ttcatcacga tcttgaggtc tggcttgaaa aactggctcc
    18241 ccatgagcct gtctctcagt atcggcataa cgttggtgaa gacaatgcag atgcccatct
    18301 taaacgatcc gtaatgggaa gggaggctgt cgttgccatt acggatggaa aattggattt
    18361 cggtccgtgg gaacagatct tttacgggga atttgatgga aggcgcagga agcgtgttct
    18421 ggttaagatt ataggcgatt agcaacctct tccgaaaatc gattacctcc cgctttccgg
    18481 gatgaggtgg ttgaaaatga cgtcacctgc aactgcacag gttcaggcaa aagggcagtg
    18541 ctcactgtca gaaggccagt tcacaccaga actctactcc tatttataaa ggcgagagtg
    18601 aatgatggct tccaaaaaaa acagtttttt tgctaccttg taaaaatgcc aaagagtgtg
    18661 tatagtgaat attaattgtt gaatctgttt tttgatgttt tacaataatg aggtgaacat
    18721 ggataataca aaaaaaatta ctatttattc gttgcctacc tgaccacact gcaagagggc
    18781 aataaagctc ctcgaggaga atggattctc ctttcaagaa ataaacgttg cctcagataa
    18841 agtgggcaga gaggaaatga ttcgtaaatc caaccagatg agcgttccag tgatcgaaat
    18901 cgatggcgaa atcaccgtag ggtttaatga agctgaatta aggaaaaaac tgggcattta
    18961 atttttcgga ggcctgtatg tatgacgtaa tgattattgg cgccggtcca tcaggcatga
    19021 cagcagcagt gtacgctgca cgaaaaaaac tccatactct cctgataagt aaagatattg
    19081 gcggtcagac aaactggaca aacagtattg agaattatat ggggtatcaa tttatagaag
    19141 gccccgagct cattgaaaag tttgaaaacc aggtaaagca atttcctatc gatcttaaga
    19201 ttggcgatgg ggttagcagc atctctctga ttgataaggg ctttgaaata cgcacagaat
    19261 caggacaaac ataccaggga aaaacaggga ttattgcaac aggaaaacgt ccgcggtctc
    19321 taaatattcc cggagaggag aagtttcgcg gaaaaggcgt ttcatactgc tcaatttgtg
    19381 atgcacccct tttcgagggg atgaacgtgg ctgttgtggg aggaggaaat tccgcccttg
    19441 aagctgctga tgatctgata aagattggtg aacatgttta tctgatttcc ctcaccccgc
    19501 tgaccggaga ccaggtcctt atcgataagg taacaaaggc tccgaatctt accgtgtata
    19561 ctgaacatga aaccctctca atagacggtg acaactttgt atcgcgtatt actgtcaggg
    19621 acttgaagtc aaacgagata agagaacttg acgtaaaagg aattttcgtt gaaatcgggc
    19681 ttatcccgaa ttcagatcta gcaaaaggtc ttgctccctt taacagattg agcgagatag
    19741 aggttaactg caattgcgaa accgttgtgc ccgggctttt ctctgctggc gatgttacaa
    19801 acactccaga aaaacagatt atcatagccg caggtgacgg tgccaaggct gcattacggg
    19861 tctaccatta tttacaaaga ttgagttgag ggtggggttc aatcattatc aatcttcaag
    19921 aatatccaat gacccgatta aagtatcata aagaaagttc catgaaacca tagtcaggta
    19981 cggcaggtac aaagcacgcc gggtctcaga aatataagag gacaagcatc tattcaggcc
    20041 ctttgtagcc agaccctgtc ttatctgttc ttcgggtcct tcggatcgcc cttgtacccc
    20101 agggctttcc agtcaagccg gcccttgtcg ctgtgacaat ctatacactt gagcgacttg
    20161 tcttttggca ccaccatgtg atgtaccggc cagaccattg atgtttctat gaaaccgaac
    20221 ttgccgctat acggaaggcc tgctgctttc ataccagagg tgatggccag gttccaatcg
    20281 tacttctgcc agtaagcgtc actgcccggt gggccaaaaa cctgggtaaa agcaatcgta
    20341 ttgtttccgc tgtcgtatgc ctgtttgcct ctcatgatct tgaatggaaa tatgcgggct
    20401 ttggggtcat tgcggcttcc cttcacagaa gtgagttgga caatctttgt tggatctatt
    20461 ttatcaccag ccagataacg tacaatattc ccgtcaaacc acatgtaggt ggggatcaca
    20521 ttcttttccc acttaaaatc tcccttcatt ttgtcatatg ttttctgacc gtacttatct
    20581 ttcggaatgt cttcaggctt cacatctttc cctgccgaag accagtccca ccagactttt
    20641 gtgggtcgat ccttagcaaa tgtaggaata tggcaactct ggcatgccac cttctcagta
    20701 tgcttattca ccagggcatt cttatggacc cggcctttgt ggcaatcgat acagtctatg
    20761 tggcttggct ctgtgtgtgt tatggtaacg gaagcagcct ctcctttcaa aaagtggtct
    20821 ttggcctgat ggcaatcctg acaggtcatt tttttcggtc cacccatatg gacatcaatc
    20881 tcagcagtcg gatttgccag cgaggtgtcc aagtcaccgt gtttgacatg atccccacca
    20941 ccaccgtaaa agtggcatga accgcatgta gcacgggacg tgcgccccac tgattgggct
    21001 acttttagca ggtcgactgg tttccaaaca ttccctttgg ggaattcttt ctgctctcca
    21061 tcataaaccg ggtgtccgga gcctgttgga aatttcttgt acatcccggt cgtatcgtgg
    21121 caaaccaggc aatccacgtt ttcttgttta gaaaaatcaa attttgcatc tttaaatccg
    21181 tatccggcat ggcaactggt acaacgaggc cagttccccg gcaggccgat acagaagttg
    21241 tttaaagcat taaccttgcc tatattaacc ttttttccgc tttccagggt ctgctctttg
    21301 gaccacgtcc agtggactgt cttcatgaag tcctttgttt ctttctcatg acactccaga
    21361 cattttctcg tgacatccgg tccggtctta aaaggtcctt cgcctacaag gtctgcatgg
    21421 ttggcccata cgaccgacac caaagcacct actgcgaacg ctgttactgc aatcatgaat
    21481 ctctggattt tcatgggttg ccttcctcct ttgctggaca ttggcaaaaa cgttgttctt
    21541 gaaaaatgat ataagagcac cttgcccatg tcaacaacaa aaaagaaaca gatgcagcaa
    21601 ctttcttaaa ataatattct ccaggaagca ggtaaccctc tctgactgtc ccagaactga
    21661 tctgaatgtc aaaacagtag caaaggtgct ttcttttaaa aaaaagatgt caaatacaat
    21721 cagcttcaaa ggcacaggca taaccattta cgccagcccc cccatctctt tacccaacgc
    21781 atttcttcta acgttccctc tcccacccgg gttcatctgc gttcatcagc gttcatcctg
    21841 tgaaaaagat gctttacaac aaatgaacaa atcagtttgt aacaatgatg catccagatg
    21901 gacatagatt gaaactcgat aatgaagact gtggctggct ttgtgatacg gcatccaaac
    21961 cacccacacg catcgcctgc cggcagtaaa gaattgcata ttgcccgaag ggctggatat
    22021 tggc
//
LOCUS       DAOOMC010000034        21377 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdus sp. isolate AMR_MDS_2685
            NODE_2405_length_21377_cov_6.842932, whole genome shotgun sequence.
ACCESSION   DAOOMC010000034 DAOOMC010000000
VERSION     DAOOMC010000034.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864760
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdus sp. (wastewater metagenome)
  ORGANISM  Syntrophorhabdus sp.
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae; Syntrophorhabdus.
REFERENCE   1  (bases 1 to 21377)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21377)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,729
            Genes (coding)                    :: 2,708
            CDSs (with protein)               :: 2,708
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 7 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21377
                     /organism="Syntrophorhabdus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2405_length_21377_cov_6.842932"
                     /isolate="AMR_MDS_2685"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2699756"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..462)
                     /locus_tag="PK125_05010"
     CDS             complement(<1..462)
                     /locus_tag="PK125_05010"
                     /inference="COORDINATES: protein motif:HMM:NF024644.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB37500.1"
                     /translation="MAEKKKKIIKTIKVDLDKCNGCRACEVICSAFHAEPKYSSNNPA
                     RSRIRMIRHPLRDIYVPVYAGEYTSAECMGRDKYIIDGKEYDECGFCRASCPSRDAFK
                     EPDSGLPLKCDMCEDLPEGEKPLCVQWCLNDALIYEEREEEVEEDVKVEDVE"
     gene            complement(500..2488)
                     /locus_tag="PK125_05015"
     CDS             complement(500..2488)
                     /locus_tag="PK125_05015"
                     /inference="COORDINATES: protein motif:HMM:NF013481.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016625 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, iron-sulfur
                     protein as acceptor [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase N-terminal
                     domain-containing protein"
                     /protein_id="HPB37501.1"
                     /translation="MRYGDTGYNLEIDLTRGNVERVETDPKLTELHLGGLGTNAKLLW
                     DRVPPETDPFAPEIPLMFSSGLLCGTPAPGANRTIITAYSPQTLLMGYSMMGGFWAPE
                     LKHAGYDKVIFRGKSPDLVYIWINNDKVEIRDASHLRGKGAVETAEIIKEELKEPNAQ
                     VAAIGLAGENRVYTASIEQGRSSASRLGLGAVMGDKGIKAIAVRGTKDIYIAKPAEFI
                     ELCNEVLKYIQYRNENPVPNVMTILQGLGSPQEMLHVDEKWHTENFMWGNSRVRRKDF
                     WNKQIEDRWREVQLDVRKRLISCYNCPMKCGAIISVPGLSTYMMKCFSKLTYTMAAFV
                     DDLDFGFKIAQRATEYGVDGFSTPQIMAFAFELWEAGILTDADFEGCPSDSEGKFYWL
                     LDRIVRREGIGDILADGTYWAAKKIGKGAEDFAHNNIKKHEQLPLKLGMLNPVYFLMY
                     ATGEKINITQIEGQFPQSPFATMEERQEFISDWIQAPDEKFKQIVLDWEFRGERSNPY
                     FPTVDMSVDIVDWQEKMHYIDDALGVCAGLSSFPLKPPYHIHNYPKIISATTGMDLDE
                     AGLTQIYNRNRNLIRAINVRRGLRRIDENPPEDHWKRRFPELEEKLLDAYYKFKGWNN
                     DGIPTKETLQALDLGYIAEDLEKRGIYDEEPSVKKASA"
     gene            3053..>3763
                     /locus_tag="PK125_05020"
     CDS             3053..>3763
                     /locus_tag="PK125_05020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB37502.1"
                     /translation="MVFSKIFSKKQSQPSDQLLSHAERELNHREQLIREKHQAVEEKL
                     RSLYEANHRLKEAESVLRKFDETERILNAKLEDLTVREEKLASDRASLEAQIEELNQK
                     LADPASQAAKIVREEMQIKSEKERLIAQEEELSQRNLQLEEKQQQLQNFEESLKVREE
                     ELSTEEMNLKKILEEAETKKRFSEEEEARVLRASDEMEERKRAIGEKAHQIDVLEQEL
                     SLRERSLEEGLANLETQRS"
     assembly_gap    3764..3863
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3864..4124
                     /locus_tag="PK125_05025"
     CDS             <3864..4124
                     /locus_tag="PK125_05025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB37503.1"
                     /translation="ELAIQEQSLAERLDKMEEREQELSLREKSLEEGLANLETQRSEL
                     AIQEQSLAERLDKMEEQVQKRAIEILKIAQSIAKKGKDAGRW"
     gene            complement(4360..4444)
                     /locus_tag="PK125_05030"
     tRNA            complement(4360..4444)
                     /locus_tag="PK125_05030"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4408..4410),aa:Leu,seq:gag)
     gene            complement(4473..4709)
                     /gene="secG"
                     /locus_tag="PK125_05035"
     CDS             complement(4473..4709)
                     /gene="secG"
                     /locus_tag="PK125_05035"
                     /inference="COORDINATES: protein motif:HMM:TIGR00810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HPB37504.1"
                     /translation="MLTVIAILHVVVSIALILIVLLQTGRGSEIGAAFGSGSSQTIFG
                     SSGSSGFMTKLTTVAVVVFMITSLVLAYFYSHKV"
     gene            complement(4709..5467)
                     /gene="tpiA"
                     /locus_tag="PK125_05040"
     CDS             complement(4709..5467)
                     /gene="tpiA"
                     /locus_tag="PK125_05040"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003498915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HPB37505.1"
                     /translation="MMRKPIVAGNWKMHNTVQESIALAKYIKDGIAGTEKTEIVLAPP
                     FTALYSVSAVIKDTKISLASQNIFYEDKGAYTGEVAPGMIKDAGCTYAIIGHSERRKY
                     FYERDEDVNLKVKKALATGLTPIVCVGETEEERVKDVTEFVVGVQVKKALFEINSLNN
                     IVIAYEPVWAIGTGKVATPMEAEEVHRFIRGVLSEMYGSASTDIRILYGGSVTPENVG
                     ELIDMEDIDGALVGGASLKPDTFLGIIRKVSEKK"
     gene            complement(5464..6654)
                     /locus_tag="PK125_05045"
     CDS             complement(5464..6654)
                     /locus_tag="PK125_05045"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006863552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HPB37506.1"
                     /translation="MKYIDQINIEGKRVFIRVDFNVPVDEHGVITDDTRIRAHLQTIN
                     YALDEHAKVIIGSHMGRPKGKKSEKHSLKPVAKRLSRLLQKDVTFLDDCVGPEVEKAI
                     SSMKNGDVILLENLRFHIGEEKNDPEFAAQLAKIMDVYIGDAFAVAHRAAASNTAITE
                     IIKECAAGYLMRNELEYFHKAMENPVRPLVAIIGGAKVSDKIGVLETLLEKVDKLIIG
                     GGMAFTFLKARQLEVGKSICEENMVELARQILDKSKTRGVKFYLPVDCVVAERPAADA
                     EVKVVTVQEIPKDWMGLDIGPATTLLFSEALQNAKTIIWNGPMGMFELDPFSRGTFGM
                     VTHVANTHALTIVGGGDTDVAVHRAGESHKISYISTGGGAFLELLEGKTLPGVAALES
                     CGGK"
     gene            complement(6727..7725)
                     /gene="gap"
                     /locus_tag="PK125_05050"
     CDS             complement(6727..7725)
                     /gene="gap"
                     /locus_tag="PK125_05050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011897301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HPB37507.1"
                     /translation="MAVKVAINGFGRIGRLVFRAGFKNKDVEFVAINDITDSKTLAHL
                     LKYDSVHGIMQADVKATENSIVVDGKEVKTFAMKEPESLPWKALGIDVVLESTGKFTD
                     RAGAEKHLTAGARKVVISAPAKNPDVTFVFGVNQEVYDKKKHDVISMGSCTTNCLAPI
                     VKILSREFGVEYGLMTTIHAFTNDQVVLDEPHKDLRRARAAALSMIPTTTGAAKAISE
                     VIPEMKGKLDGMAIRVPTPNVSLVDFVATLSKATTRQEINAKFKEYSAGPMKGILYCS
                     EEELVSKDFNGNPHSSIVDMPNTTVIGGTMVKVLSWYDNEWGFSNRMLELLSFIMK"
     gene            complement(7939..8475)
                     /locus_tag="PK125_05055"
     CDS             complement(7939..8475)
                     /locus_tag="PK125_05055"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013250.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HPB37508.1"
                     /translation="MNIPNLLTIFRLFLTSFFILAIHYGEFRIALWLFVVQAITDLLD
                     GFLARVLGEKTSLGALLDPLADKTMLVSSFIVLYMKGIVPLWVTFIVLIRDFVLTFGY
                     LILCKLFGKIEVIPSTLGKITTFCQIGTVVYVLWSDTRSYQDLFFYPTVALTIITGLH
                     YVYQGILIIKNKGRVTVT"
     gene            complement(8472..9845)
                     /gene="dnaB"
                     /locus_tag="PK125_05060"
     CDS             complement(8472..9845)
                     /gene="dnaB"
                     /locus_tag="PK125_05060"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF004384.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HPB37509.1"
                     /translation="MARITKNTREAKAQTAGHIPPQNIEAEQSLLGGLLVDPESINKI
                     VDIVGPRDFYKDAHARIYDVMLDLYERNEAIDIITVSSLVRDKGLVEKIGGITYLNSL
                     VDLMPSSANIVQYAKMVGEKALMRNLINAATLIIEKGHELNLNVDSYIDEAENLIFQV
                     AENKFKPSFFSMKDIVMENIQKIELLSQKKQSVIGVPTGFIELDKLTSGLQPSDLIIV
                     AGRPSMGKTAFCLNIAQHVAAMKEPSTPVGVFSMEMSKEQLVTRLLSSESEIDHSKLR
                     TGMLSQSDWRRLSEAASKLLETSMFIDDSPSMSVLEVRARARRLKKEHGLGLLIIDYL
                     QLMKGRTFIESRVQEISEISRSLKALAKELNIPVIAISQLSRAPEKREHEAKKPRLSD
                     LRESGAIEQDADVILFIYRDEVYNKESEKKGTAEIIIGKQRNGPTDTIELAFIDKFTA
                     FRNLFRE"
     gene            complement(9846..10298)
                     /gene="rplI"
                     /locus_tag="PK125_05065"
     CDS             complement(9846..10298)
                     /gene="rplI"
                     /locus_tag="PK125_05065"
                     /inference="COORDINATES: protein motif:HMM:TIGR00158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HPB37510.1"
                     /translation="MKVILTEDVKGTGKKGEVVVVKDGHGRNFLIPRGLALPATEGNV
                     KRFENIVRSIQNKRGRDIKVAGELKEKLEEISLTIKKKAGVDGKLFGSVTHMDIVEAI
                     KNVTGAEVDKKNVRLEEPIKMTGAYTVEIHLEQNINATIKIDVEQEAG"
     gene            complement(10311..10580)
                     /gene="rpsR"
                     /locus_tag="PK125_05070"
     CDS             complement(10311..10580)
                     /gene="rpsR"
                     /locus_tag="PK125_05070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012096455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HPB37511.1"
                     /translation="MARPTRPARGHDSTKKRVYVRKRVCRFCQDPNIAIDYKDPKLLK
                     HFITERGKIMPRRMTGTCANHQRKLKEAINMARHIAFLPFTTLSR"
     gene            complement(10641..10850)
                     /locus_tag="PK125_05075"
     CDS             complement(10641..10850)
                     /locus_tag="PK125_05075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB37512.1"
                     /translation="MNRVFLSGTLKKQPEVVYTPRGEKLIKFPLWVDEGGFGIDVVYI
                     GNQGTKRLAGAPGCSVIVAGQLART"
     gene            complement(10847..11200)
                     /gene="rpsF"
                     /locus_tag="PK125_05080"
     CDS             complement(10847..11200)
                     /gene="rpsF"
                     /locus_tag="PK125_05080"
                     /inference="COORDINATES: protein motif:HMM:TIGR00166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HPB37513.1"
                     /translation="MGRYENIIVINPDCTKEEEEELLKRITVNIEKFGANIVKVDDWA
                     VRKLAYPIKKKDKGHYFFFLLDMAEENVALLNKFYRTIDLILRHMFVVVTESNKGLEK
                     PPEPVVFDELEGEFA"
     gene            complement(11216..11527)
                     /gene="yajC"
                     /locus_tag="PK125_05085"
     CDS             complement(11216..11527)
                     /gene="yajC"
                     /locus_tag="PK125_05085"
                     /inference="COORDINATES: protein motif:HMM:TIGR00739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HPB37514.1"
                     /translation="MNIAYAMGSQGAAGQGGSQWMSFLPLILLFVVFYFLLIRPQQKR
                     AKQQKQFLENLKKGDDVITSGGLYGKITGLTDSTVTIEIAEKVRVKVAKSAVAQQAPQ
                     Q"
     gene            complement(11502..12638)
                     /gene="tgt"
                     /locus_tag="PK125_05090"
     CDS             complement(11502..12638)
                     /gene="tgt"
                     /locus_tag="PK125_05090"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: protein motif:HMM:TIGR00430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - queuine tRNA-ribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HPB37515.1"
                     /translation="MNTIHILKEEGRARLGIVRTQHGDIDTPVFMPVGTQGIVKATSP
                     KDLKEMGVRIILANAYHLYLRPGDALIQEMGGIHRFAGWNGAVLTDSGGYQIFSLGVL
                     REIREDGVLFQSHIDGSRHFLTPEKVIRIQENIGADIIMCLDECIPYPSSYEYTKSSV
                     GLTSRWARRCKEAKKPDDSMLFGIVQGGFYKDLRLKSAQELIDMDFNGYALGGLSVGE
                     PKSIMWEMIDTVVDVLPPNKPKYLMGVGFPEDIVEGVYRGIDMFDCVIPTRHARNGSL
                     FTREGRINIKHARYTNDESPIDERCTCYTCRTFSRAYLRHLFVSHELSSYFLNTVHNI
                     FYYNNLLKTIREALQTGSFEVFYEQFKTQWKGGETQNEYSVCNG"
     gene            complement(12635..13660)
                     /gene="queA"
                     /locus_tag="PK125_05095"
     CDS             complement(12635..13660)
                     /gene="queA"
                     /locus_tag="PK125_05095"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: protein motif:HMM:NF014592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HPB37516.1"
                     /translation="MGINIEEFDYSLPKEFIAQYPEKEREASRMLVFNRADGSMTHRA
                     FRDVTQYLKESDVLVLNDSKVIPARISAKKPTGARVDILLIEKINDKSWFCLVNGVKG
                     GVRELKVLIGHREATLRRGIESWIIEFDQNREMFDIMGEYGKMPLPPYVKRKDGDGAI
                     DIDRYQTVYAEMPGSIAAPTAGFHFTEQLLKVIRQTGVNVLKITLHIGIGTFFLIKTQ
                     NVADHRMHGEYYHVTPEAKAFIRKAKDEGRRIIACGTSVVRTLESLYERNGSAPLAGK
                     TELFIYPGHHFNVVDALITNFHLPRSTPLLLVSAFAGTRETMRCYTEAIEKGYRFYSY
                     GDAMFIL"
     gene            13833..14654
                     /locus_tag="PK125_05100"
     CDS             13833..14654
                     /locus_tag="PK125_05100"
                     /inference="COORDINATES: protein motif:HMM:NF020252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HPB37517.1"
                     /translation="MENLREELIDKAKTLRVLPTLHSIIERLFVILDDKNSSFSDLND
                     VIKYDQAISSKIISIANSAYYSRGIEIFSLQRAMITMGFEEVKNIVMCLVFVENVLKE
                     LKLRTDIILELWKHSVFVACAARTLAGRMFSEDPQKVFTIALLHDIGKIIFYITVENY
                     NELIAKVHSKGKSLNREEREIYGTDHEELGYILSVKWQFPEEFSQIIRYHHDGKVAGK
                     HESLLRLVRAADRFSTASVHDSEPETFILLKERDGIMKEVEKIMNIFQLNSHGTR"
     gene            14641..16593
                     /locus_tag="PK125_05105"
     CDS             14641..16593
                     /locus_tag="PK125_05105"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HPB37518.1"
                     /translation="MERDNLKSESINFQWNFYERAINNYSIIMRMLHFVEEEESLVNN
                     MPRILVEGSIFDMCKILRNDNGIEREGFFDIEESLNSIDLNAIKSKCAGVFTPSVLKD
                     AFGYGTLYVYPLKKDMDIVGFLLLGRRLPIELDARTLRELEIVCDIYNKSLILHINIH
                     NHKKAAKRKSIFESLINEFPDALVMIDRNGSIVFANRRAKSEFEGGKGFLVGEKIDSV
                     ISGIGNDFYRKEMALHGEVKYKSGDKFKIFRVESFPLKEAAEKGVWKGLLFKDVVEKK
                     IKEEEYVRKEKMESIGMLAGGIAHDFNNLLTGVLGYASLIKNFLSNEEKLYRYAEAIE
                     SSAQRAAKLAQHLLNFSRRQRRVSGVVDINMLLEDILFLIKESFRDIEIEKSLDAGLY
                     PIKGDEGELQNVFLNLCTNAKDAITGKGKLRVRTERKRYVGHREFALIEIEDTGQGID
                     EDIKEKIFEPYFTTKDNGTNLGMGLYLVDKIIREHGGFVELQSEKNKGTKFSIYLPLP
                     TKIEEPVPVKEEPANDGLTKKQTILVVDDEDVVRELIKGVLADEPTDILEAPDGTQAI
                     EIFKDKYASIDLVILDMIMPGIKGDEVLREIRSIRKDIRVIIASGFMSEEQRDRLKQY
                     KVDAFLDKPFRDKDVIQTIRDVLSKK"
     gene            16684..17721
                     /gene="rlmN"
                     /locus_tag="PK125_05110"
     CDS             16684..17721
                     /gene="rlmN"
                     /locus_tag="PK125_05110"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: protein motif:HMM:TIGR00048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HPB37519.1"
                     /translation="MQNFFELTLHQLENAIGAIGNEKFRARQLYKWIYNKGVFDFHEM
                     TNISKSLRLIFKDMFCTDPPEIKDILRSNDGTIKFGITASDGRTMESVLIPEKGRNTL
                     CLSSQIGCRMGCKFCVTGKIGFVRNLTSAEIIGQIMAVKRYLGEQKITNIVFMGMGEP
                     VDNLDTLLHTLEIMKNPLGLDFSHRRITVSSVGLLEGLRTLEPKVAGLAISLNAADDG
                     KRTYLMPINRLYPIRDIIGFVREFRGSKRVRITFEYVLIKGFNDSLDDAQQLAQLLTG
                     VKCKINLIPFNESPYVEFKTPDTKAVNQFHDYLLQRHFTAIVRDSRGQDIGAACGQLG
                     ARYLGTLQEIN"
     gene            17883..18314
                     /locus_tag="PK125_05115"
     CDS             17883..18314
                     /locus_tag="PK125_05115"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HPB37520.1"
                     /translation="MRLNVMYEDHTVYCDIVVDNRFEGYHDVVHGGMLFGILDVLMWH
                     IIFMETKKVGMTRKTEMEFFKPVMCNVQYRAIGKFDRVVGRDIYASAWIEDREGECYA
                     RVTALFREGKNLSAPDFLKDFDFSKTTPEIKARFMSLLEDQ"
     gene            complement(18384..19859)
                     /gene="hflX"
                     /locus_tag="PK125_05120"
     CDS             complement(18384..19859)
                     /gene="hflX"
                     /locus_tag="PK125_05120"
                     /inference="COORDINATES: protein motif:HMM:TIGR03156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HPB37521.1"
                     /translation="MIEYVVVGDKNGIRVPTPSTERVGRARFRGVRFVHTHLNGELLS
                     KEDLTDLALLQLDLVACITERRDEGLVIHVGYLIPQNKKGQAWDFIGPVPIQEFDLDF
                     IEFITELENEFVKERGRYYVLDKDRERCILVSVTSPRWKQDVADHIVEMKDLCYSAGI
                     TVVETIIQRPKEVHPKFLIGKGKIEEIVVRCQQLGVDLLVFDDELSPGQIKNISSMTD
                     LRVIDRNQLILDIFAGRARTNEAKIQVELAQLRYIRPRLAEANIAFSRLTGGIGARGP
                     GETKLEVDRRRIRDKISFLEKKLNEVRLARDKKREKRRYSGIPIVSIIGYTNSGKSTL
                     MNLLTKSKVEAEDKPFSTLNPTTRKIKYPERKNIILTDTVGFIRDLPQVLLRAFIATL
                     EELDDAHLLLHLVDVSSPDFEDRIETVEELLVPLGLKDKRRLIVFNKIDKINGSFLRN
                     VEERYRAVSISSVKKIGIERLLQTIETELASMGLVNEKLNG"
     gene            complement(20064..20558)
                     /locus_tag="PK125_05125"
     CDS             complement(20064..20558)
                     /locus_tag="PK125_05125"
                     /inference="COORDINATES: protein motif:HMM:NF014417.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination NusG
                     family protein"
                     /protein_id="HPB37522.1"
                     /translation="MKRWYVVNTKPRNEDRAAMNLVNGGIEVLAPRLRLRKYREGKFV
                     QVIEPMFPGYIFVRFHPIDEFRMVKYARGVKTIVHFGGTIVPLQDEMIDFIRARLEDG
                     VASIEKPKIDKGEKILIKDGPFKGFSGIFESELDGKERVAILLEGVHYCAKMEIDRDL
                     IDRA"
     gene            20754..20827
                     /locus_tag="PK125_05130"
     tRNA            20754..20827
                     /locus_tag="PK125_05130"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:20788..20790,aa:Arg,seq:ccg)
BASE COUNT         5783 a         4784 c         4544 g         6166 t
ORIGIN      
        1 ctctacgtcc tcgaccttta cgtcttcttc cacttcttct tcccgttctt cgtagataag
       61 ggcatcattt aaacaccact gaacacacaa aggcttctcc ccttcaggga gatcttcgca
      121 catatcgcat ttcaacggaa gacctgagtc aggttctttg aaggcatccc tcgatgggca
      181 ggaggccctg cagaagccac actcatcgta ttccttgccg tcgattatgt atttgtcccg
      241 gcccatacac tcggcagagg tatactcacc ggcatatact ggaacatata tgtctctaag
      301 tggatggcga atcatccgaa tacgggacct cgcagggtta ttgctgctat attttggctc
      361 agcatggaac gctgagcaga tcacttcgca tgccctgcaa ccattacatt tgtcaagatc
      421 aacctttatt gttttgatga tttttttctt tttttcagcc ataacggttt ccacctatct
      481 actttatttg tttcaatgct tacgctgaag cctttttgac agaaggttct tcatcgtaga
      541 tacctctttt ttctaaatcc tcagcaatgt agcccagatc taacgcctgt aaagtttctt
      601 ttgtaggtat gccatcgttg ttccatccct taaacttgta ataggcatcc aagagttttt
      661 cttcgagttc tggaaatcgt ctcttccagt gatcttccgg cggattctca tcgatccttc
      721 ttaagcctct tctaacatta atagccctta tcaggttccg gtttctattg tatatttgtg
      781 taagaccagc ttcatccaga tccattccgg tagttgccga aatgattttc gggtagttat
      841 gaatatgata gggtggtttc agtggaaacg atgataatcc ggcgcacaca cccagagcat
      901 catcaatgta gtgcatcttc tcctgccaat caacaatgtc aacggacatg tcaacagttg
      961 gaaaatacgg atttgaccgt tctcctcgga attcccagtc cagaacaatc tgtttgaatt
     1021 tttcatcagg agcctgaatc cagtcgctta taaactcctg cctctcttcc atggttgcaa
     1081 agggtgactg gggaaactgc ccttcaatct gggtgatgtt aattttctcg ccggtggcat
     1141 acatgagaaa gtaaacaggg ttcagcatac caagcttgag aggtagctgt tcatgttttt
     1201 tgatattatt atgggcaaaa tcttctgccc ctttaccgat ttttttagca gcccaataag
     1261 tgccgtctgc cagaatatcg ccaatccctt cccggcgtac aattctatcg agcagccagt
     1321 agaatttccc ttcgctatct gatggacatc cttcaaagtc tgcatcggtc aagattcctg
     1381 cttcccacag ctcaaaggca aaggccatga tttgaggggt tgagaaccca tccacaccgt
     1441 actcagtagc acgctgtgcg atcttaaaac caaagtcaag gtcatctaca aaagctgcca
     1501 tcgtatatgt gagttttgaa aaacatttca tcatatacgt tgaaagccct ggtacagaaa
     1561 ttattgctcc gcatttcata gggcagttgt agcaactgat taatcgtttt cggacatcta
     1621 actgaacttc tctccatcta tcctcaatct gcttattcca aaagtctttt cttcgaactc
     1681 gggaatttcc ccacataaag ttttcagtgt gccatttctc atcaacatgc agcatttctt
     1741 gtggagaacc aagcccctgc aaaatggtca ttacgtttgg cacgggattt tcgttccgat
     1801 actgaatata tttcaatact tcattacaga gctcaataaa ttcagctggt ttggcaatgt
     1861 aaatatcttt ggtaccccgg acagctatgg cttttatccc cttgtctccc ataacggcgc
     1921 ctaacccgag tcggctggcg ctggacctgc cctgttcaat ggaagccgta tagaccctgt
     1981 tttcaccggc caggccaata gctgccacct gtgcattggg ctctttcaac tcttccttga
     2041 ttatttctgc agtttcaaca gcgcccttac ctcgaagatg acttgcatcc cgtatttcaa
     2101 ccttgtcatt gtttatccat atatacacaa gatcaggtga cttcccacga aagattacct
     2161 tgtcgtagcc tgcatgtttc agttcaggcg cccagaaccc acccatcatc gaatatccca
     2221 ttaacagggt ctgaggggag taggcagtaa taatagtacg attggcgccg ggagcaggtg
     2281 taccgcacaa aaggccgctg ctgaacatca gtggaatttc gggagcaaag ggatcggttt
     2341 caggaggaac cctgtcccac aagagctttg cattggtgcc cagtcccccg agatgcagtt
     2401 cggttaactt cgggtcagtt tcaacccttt caacatttcc tcgggtgaga tcaatttcta
     2461 aattataccc cgtatctccg tacctcatgt tttaactcct ttatatataa ggttcttttt
     2521 actgttttac ttgtgtattg attgtttctg ccttgttata attgtaacaa tttgaaaaat
     2581 acttgtcaac agaagaaaag aaataattca caagcaacat tttttacgtg aatgagaatt
     2641 gtcaaattat tccttttttc gtttatcatt ctttattttg atatgagttc atccagatgt
     2701 gttttaatct gcagtaatat aattgtgtac aatatggatc ataatgaacc aaaaatgttt
     2761 tggtggaaaa gcttcagctc gatgatattt gcacgccatc atcagcggca tgtgtttgac
     2821 cgaaaagctt cctatttgat tgggaacgta gacgccatgc ttgacaatca gctgaaatag
     2881 aaagataatg ctaataaatt taagaacagg acacattgcc ggaataaccc gtgcacacac
     2941 gagaaaaacg aatgaataag gggtattcgg tttaattcgt gtgcaaaatc gagtggttct
     3001 ctcgcaggaa gtaattgttt ttacaaatgg ttgatcatat tggagggaaa agatggtttt
     3061 ttcaaagatt ttttcaaaaa aacagtcgca accatcggac caacttttga gtcatgctga
     3121 acgggagtta aatcatcgcg aacagcttat acgagaaaag caccaggctg tagaggagaa
     3181 gttgcgaagc ctttacgaag ccaatcatcg gctcaaagag gcggaatcag tgctgagaaa
     3241 gtttgatgag accgaacgca tcctcaatgc caagctcgaa gacctgacgg taagagagga
     3301 gaagctggca tctgaccgcg cctcattaga ggctcaaatt gaggaattaa accaaaaatt
     3361 ggcggatcct gcatcacagg ctgcgaaaat agtaagagag gaaatgcaaa ttaagtctga
     3421 aaaggagaga ttaatcgctc aagaagaaga gttgtcccaa agaaatcttc agcttgaaga
     3481 aaagcaacaa cagcttcaga atttcgaaga aagcctgaag gtacgtgagg aagagctttc
     3541 aaccgaagag atgaacttga agaagatcct ggaagaagcg gaaacaaaaa aacgtttttc
     3601 tgaggaggag gaagctagag ttctgcgcgc ttctgatgag atggaagagc gtaagcgtgc
     3661 gattggcgaa aaagcacatc aaattgacgt gcttgaacag gaactcagcc tcagagaaag
     3721 gtctttagag gaagggctgg ccaacctgga gacccagcgt tcannnnnnn nnnnnnnnnn
     3781 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3841 nnnnnnnnnn nnnnnnnnnn nnngagctgg caatccagga acagtccctt gccgaacggc
     3901 tggataagat ggaggaacgt gaacaggaac tcagtctcag agaaaagtct ttagaggaag
     3961 ggctggccaa cctggagacc cagcgttcag agctggcaat ccaggaacag tcccttgccg
     4021 aacggctgga taagatggag gaacaggttc agaagagggc catcgaaatc ctgaagatag
     4081 cccaatccat agcaaagaaa ggtaaggatg ctggcagatg gtaggccagt atcggagtgt
     4141 ttgaatactg ttggctttcc tgcctatttc tacagggtag gagagagact gatgttcagg
     4201 aagggaatat gcgaaatgag gctgtcatat cgattccatt gttgcaatcg attaatggtg
     4261 ttgagaatag cgtcgggtaa acagattctc tgcagacatt ttttgataaa aaaatagggg
     4321 ttataattat ataaccccct aaaattataa gttatttaat ggtgccgaag gcgggatttg
     4381 aacccgcacg agtctccccg ccaccccctc aagatggtgt gtctaccaat tccaccactt
     4441 cggcatcgat gttttttatc ataaacttaa aactaaacct tgtgactata aaaatatgca
     4501 aggacaaggc ttgtaatcat aaataccacc acagctaccg ttgtcagttt tgtcatgaag
     4561 cctgaagatc ctgaactacc aaaaatcgtt tggcttgatc cgctaccaaa ggctgctcct
     4621 atttcagaac ctctccctgt ctgaaggaga acaatcagaa ttaaggctat tgaaacaaca
     4681 acatgtagta ttgctatcac tgtaagcatt attttttctc cgagactttt ctaattatac
     4741 caagaaatgt atccggtttc aagcttgccc cgccaacgag tgctccgtcg atatcttcca
     4801 tatcaattaa ctcaccgaca ttttcgggag ttacgctccc gccgtataga atccttatgt
     4861 ctgtgctcgc gctgccatac atctcgctga gaacacctct aataaaacga tgaacctctt
     4921 cagcttccat gggcgtagct accttccctg tgccaatggc ccatactggt tcataggcaa
     4981 tgacgatgtt attcagagaa tttatttcaa agagagcctt ttttacctgt acgccaacca
     5041 caaactcggt gacatctttt acacgttcct cttctgtttc tcctacacag acaatgggcg
     5101 ttaaccctgt agcaagtgct tttttcacct tcaggtttac atcttcatca cgctcgtaga
     5161 aatatttccg cctctcggag tggccaataa ttgcataggt acatcctgcg tccttaatca
     5221 ttccaggggc cacttcccct gtataagcgc ctttatcctc gtaaaagata ttctgtgagg
     5281 caagcgatat tttggtgtct tttataacag cagaaacgct atataaagca gtaaaaggag
     5341 gggccagtac tatttcagtt ttttctgttc cagcaatacc atcttttata tactttgcca
     5401 gtgcaattga ttcttgaact gtgttgtgca tcttccaatt gcctgcaacg ataggttttc
     5461 tcatcattta cctccgcatg actcgagggc tgccacacct ggcagggtct tgccttcgag
     5521 aagttcaagg aatgctccgc caccggtgga aatataagaa attttatgac tctcgcctgc
     5581 gcgatgaact gcaacatctg tgtcaccacc acctacgatg gtcaaggcat gagtattggc
     5641 gacatgtgtc accattccaa aggtacctct gctaaaagga tcgagttcaa acatacccat
     5701 tgggccattc cagatgatcg tcttggcgtt ttgtaaagct tctgaaaaaa gcagtgtggt
     5761 tgcagggcca atatcgagtc ccatccaatc tttggggatt tcctggacag tgaccacctt
     5821 tacttcggca tctgccgctg gtctctccgc tacaacgcag tcaacaggga gatagaattt
     5881 gacgcctctg gtttttgact tgtcaaggat ttgtcttgca agctcgacca tattttcttc
     5941 gcagatcgat tttcccactt caagctgcct ggcttttaag aatgtaaaag ccatgccgcc
     6001 gcctattata agcttatcaa ccttttcaag caatgtttcg agaaccccta tcttgtctga
     6061 tactttcgcg ccaccgataa ttgccacaag tggcctcaca gggttttcca tggctttgtg
     6121 aaaatattcc agttcatttc tcatcaaata ccctgctgcg cactccttga tgatttcagt
     6181 gatcgctgtg ttcgatgctg cagccctgtg tgcaacggca aaggcatcac caatatatac
     6241 atccatgatt ttggcaagct gagcagcgaa ctcagggtcg tttttttctt ctcctatatg
     6301 aaacctgaga ttttcaagga gaatcacatc accatttttc atgctggaga ttgctttttc
     6361 aacctcaggg cccacacaat catcaagaaa ggtaacatct ttttgcagaa ggcgggaaag
     6421 cctttttgct acaggcttta aagaatgctt ctctgatttc ttgccttttg gtctgcccat
     6481 atgagaaccg atgataacct ttgcgtgctc atccagggca tagttgatcg tttgaagatg
     6541 agcacggatc cgtgtgtcat ctgtgatgac gccgtgctca tcaacaggta cattgaaatc
     6601 gactctgata aaaaccctct ttccttcgat gttaatctgg tcaatatatt tcatatctac
     6661 ctcttatgta tttacagatg aggaaatgaa catgcatggt tttccagtgt ctcatctgtt
     6721 gtaatgctat ttcataatga atgacaggag ttccagcatt ctgtttgaaa aaccccattc
     6781 attatcatac caggaaagca ccttgaccat tgtgccccct ataacagtcg tattgggcat
     6841 atcaactata gaagaatggg gattcccatt aaagtctttc gatacgagtt cctcttcaga
     6901 gcagtagagt atgcctttca tcggaccggc agagtattct ttgaattttg cgtttatctc
     6961 ctgtctggtc gtggcttttg acagcgtggc aacaaagtcg acaagagata cattgggtgt
     7021 gggtaccctg atggccatac cgtcaagctt tcctttcatc tccggtataa cctcggaaat
     7081 cgcttttgca gcacctgtag ttgttggtat cattgacagg gctgccgccc tggcacggcg
     7141 caggtcttta tggggttcat ccaggacgac ctggtcattt gtaaaggcat gaatagttgt
     7201 catgagtcca tactcaaccc caaactctct ggagagtatc ttgacaatag gtgcaaggca
     7261 atttgttgta caggatccca ttgaaataac gtcatgtttt ttcttatcat aaacttcctg
     7321 gttgaccccg aaaacaaatg tcacgtcagg gttttttgca ggggccgaaa ttacaacctt
     7381 cctggcaccg gctgtcaggt gtttctctgc tcctgccctg tcagtaaact ttcctgtgct
     7441 ttcaagaacc acatcaatac ccagggcttt ccaaggtaaa gactcaggct ccttcattgc
     7501 aaaggttttt acttcttttc catcgacaac gattgaattc tcagtagcct tcacatcagc
     7561 ctgcatgatt ccatgaacgg aatcatattt taaaaggtgt gcaagtgttt ttgagtcggt
     7621 tatatcattt attgcaacga attccacatc tttattttta aaacctgctc tgaagactaa
     7681 cctcccgatc cttccaaaac cgtttattgc aaccttcacg gccataagaa cctcctcctt
     7741 tttttcttta tactacaaag aaaaagcctt tgctgtcaat gatattgagt aaacaagtgg
     7801 gaaaaacgtg acttgctgga aacgcgggaa aggaagaaag aagtaaaggt gagaaaagcg
     7861 aaaagtatag aaatgatatg gagagaagtg caaagcctgt gacaggcctg ctatgagctt
     7921 acagctatct gctaattact atgttacggt tacccggccc ttgttcttaa tgatcagaat
     7981 tccctggtaa acataatgca ggccggtaat aatagttaga gcaacagtag ggtagaaaaa
     8041 aaggtcctga taactccgtg tatctgacca caaaacatag actactgttc ctatctggca
     8101 gaatgttgtt atctttccaa gggtactcgg aatgacttca atcttaccaa ataatttaca
     8161 caggattaga taaccgaagg ttagaacgaa gtcccttatg aggacaatga atgttaccca
     8221 gaggggcacg atacctttca tataaaggac aataaatgag gatacgagca tcgttttatc
     8281 cgccaaaggg tcgaggagtg ctccaagact tgttttttcc cccaggaccc tggcaagaaa
     8341 cccgtccagt aaatccgtta ttgcctgaac cacaaacagc cagagggcga ttctgaattc
     8401 tccgtagtga atggcaagaa taaaaaaact agttaaaaaa agccggaaga tagtgagaag
     8461 attcgggatg ttcattctct gaataggttt cggaaagcgg taaatttatc tataaaggcc
     8521 agttcgattg tgtctgtagg tccattacgc tgtttcccga taatgatctc tgctgtgcct
     8581 tttttttcgg attccttgtt gtagacttca tccctgtaga taaacaggat aacatctgcg
     8641 tcctgttcga tagcgccgga ttcccgcagg tcggaaagcc gaggtttctt ggcctcgtgc
     8701 tctctttttt ctggtgccct gctcagctgt gatatggcaa tgaccggaat atttaattct
     8761 tttgccagcg ctttgagtga ccgcgatatt tcggatatct cttgcacacg gctttctatg
     8821 aaagttcttc ctttcatgag ctggaggtaa tcgataatca ataacccaag accgtgttct
     8881 tttttgagcc tccgggcacg cgccctgacc tccaaaacac tcattgaagg tgagtcgtcg
     8941 atgaacatgg atgtttcgag aagtttgctt gccgcctcag agagccttcg ccagtcactt
     9001 tgtgagagca ttcctgtcct aagcttagaa tggtcaatct ctgattctga gctaaggagt
     9061 cgggtcacaa gctgttcttt ggacatttcc atggagaaaa cacctaccgg cgtagagggt
     9121 tctttcatgg cagcaacatg ctgtgcaata ttgagacaaa atgccgtttt tcccatactt
     9181 ggcctgcccg cgactatgat caggtctgat ggctgtaggc cgcttgttaa cttgtcaagc
     9241 tcaatgaagc cagttggtac gccgataaca gattgttttt tttgtgacaa aagttctatt
     9301 ttctgaatat tttccatgac tatatccttc atggaaaaga atgatggctt gaatttgttt
     9361 tcagctacct ggaagatgag gttttccgcc tcatcgatat aggagtccac atttaggttt
     9421 aattcatgac ctttctcaat gatgagggta gctgcattga tcaggtttcg catgagcgcc
     9481 ttctccccca ccatctttgc atattgaaca atattggcgg agctgggcat caggtccacg
     9541 agactgttaa gatacgtgat tccccctatt ttctcgacga gacctttgtc gcgcaccaga
     9601 ctgctaaccg taataatgtc gatggcctca ttgcgctcgt aaagatcgag catgacatcg
     9661 taaatccttg cgtgggcatc cttataaaaa tcacggggcc ccacaatatc tactatttta
     9721 ttgattgatt cgggatcgac gagaaggccg ccaagcagag actgctctgc ttcgatattc
     9781 tgcggtggta tatggcccgc tgtttgtgcc tttgcttctc gtgtattctt ggtgattcgg
     9841 gccatttacc ctgcttcttg ttcaacgtct atcttgatag ttgcgttgat gttttgttca
     9901 aggtggattt caacggtata tgccccggtc atctttattg gctcttccag cctcacgttt
     9961 ttcttgtcca cttctgcacc cgttacattc ttgattgctt ctacgatgtc catgtgggtt
    10021 acagagccaa aaagttttcc atcaactccg gcttttttct tgatggtgag agaaatctcc
    10081 tcaagcttct ctttcagctc accagccact ttgatatctc ttccgcgttt gttctgaatg
    10141 ctcctgacta tgttttcaaa ccgcttgaca tttccctctg ttgcagggag agcgaggcca
    10201 cggggaataa gaaaatttct tccatgaccg tcctttacca ctacaacctc accctttttt
    10261 cctgtacctt tcacgtcttc tgtaagtatt accttcatgt ataacctccg ctaccttgat
    10321 agagtcgtaa aaggaaggaa tgcgatatgt cgtgccatgt tgattgcttc cttcagtttt
    10381 ctctgatggt tggcgcatgt gcctgtcatt cttcggggca ttatcttccc cctctcggta
    10441 atgaagtgtt tcaagagctt cggatcttta taatcgattg ctatattggg gtcctggcag
    10501 aatctgcaaa ccctttttct tacataaacc ctcttttttg ttgaatcatg accacgggct
    10561 ggtcttgttg gtcttgccat ctattcctcc atccatataa ttttgttggc cataagcctt
    10621 aagctatcct gtggctttcc ttaagttctg gcaagctggc ctgcaacgat gacactacat
    10681 cctggcgctc cggcaagtct cttggttccc tggttcccaa tgtataccac atcgatacca
    10741 aagccacctt cgtctaccca taaggggaat ttaataagtt tttccccccg tggcgtgtag
    10801 acaacttcag gctgtttctt gagcgtgcct gagagaaaaa ccctgttcaa gcaaactcac
    10861 cttcaagttc gtcaaaaaca accggctctg gtggtttctc cagacctttg ttggattccg
    10921 tgacaacgac aaacatatgt ctgagaatga ggtcaatagt ccgataaaat ttgtttaaca
    10981 gggctacatt ttcctcagcc atgtcgagga ggaaaaagaa gtaatgtcct ttgtccttct
    11041 tttttatcgg atatgcgagc tttcttactg cccaatcgtc aactttaaca atattcgcgc
    11101 caaatttctc gatatttacg gtaatcctct tgaggagttc ttcctcttct tcctttgtac
    11161 aatccgggtt gatgacaatg atgttttcgt acctacccac tttcctctcc tttcgctatt
    11221 gttgtggcgc ctgctgtgct actgcgcttt ttgctacttt tacccgtacc ttttctgcaa
    11281 tctcgatagt gaccgtcgaa tctgtcagac ctgttatttt accatacaag ccgccagatg
    11341 taataacatc atcgcctttt ttcagatttt ccagaaactg tttttgttgc tttgctcttt
    11401 tctgctgtgg tctgataagc aggaaataaa aaacgacgaa taagaggata aggggaagaa
    11461 agctcatcca ctgactgccc ccctgtcctg ctgctccctg gctacccatt gcatacgcta
    11521 tattcattct gtgtttcacc tcctttccat tgtgttttaa attgctcgta aaaaacctca
    11581 aatgaccctg tttgcaaggc ttctcttata gttttcagca aattattata atagaaaatg
    11641 ttgtgtactg tgttgagaaa ataactcgac aactcgtggg agacaaaaag atgtctcagg
    11701 tatgcccttg aaaaggttct gcacgtataa cacgtgcatc tttcatcaat ggggctttca
    11761 tcgtttgtat atctggcatg cttgatattt atccttcctt ctcgggtaaa aaggctccca
    11821 ttccgtgcat gccgggtagg gataacgcaa tcgaacatgt caatgcccct gtacacccct
    11881 tccactatgt cctcggggaa tcccaccccc atcaggtatt taggtttgtt tggggggagc
    11941 acatcaacca cggtgtctat catctcccac ataatacttt ttggttcgcc cacactcagc
    12001 cctccgaggg cgtaaccgtt gaaatccatg tcaatcaatt cttgggccga ttttaaccgt
    12061 aaatccttat aaaaacctcc ctgaacaatg ccaaagagca ttgagtcatc aggttttttt
    12121 gcttccttgc atcttcgggc ccatcgcgat gtaaggccta ctgagctctt cgtgtattcg
    12181 tagctcgagg ggtagggaat gcattcatca aggcacatga tgatatctgc ccctatgttt
    12241 tcttgaatcc tgataacctt ttcaggagtc aggaaatgcc gtgatccatc aatatgagac
    12301 tggaataaaa cgccatcttc cctgatttcc cgaagtaccc ccaaactaaa aatctgatat
    12361 ccgccgctat cggtgagcac tgcaccgttc cacccagcaa atctatgtat accccccatc
    12421 tcctgtatca gggcatcccc tggccttaaa taaagatgat aggcgtttgc cagaatgatc
    12481 ctgactccca tctcttttaa atccttgggt gaagtggctt tcacaatgcc ttgtgtgccc
    12541 actggcatga aaaccggcgt atctatgtcc ccgtgctgcg ttctgacaat ccccagacgt
    12601 gcgcgtcctt cttctttgag aatgtggatt gtattcatag gatgaacatt gcatccccat
    12661 aactgtagaa cctgtatcct ttctctatcg cctctgtgta acatctcata gtttcacggg
    12721 ttcctgcaaa ggcagaaacg agtaggaggg gcgtagaacg gggcaaatga aaatttgtaa
    12781 taagtgcatc aacaacgtta aaatgatgac cggggtatat aaaaagctct gtttttccag
    12841 caagcggtgc gcttccattc ctttcgtaaa ggctttcaag ggtacgcacc acgcttgtgc
    12901 cacatgctat gattctccga ccctcatcct ttgcctttct tatgaatgcc ttagcctccg
    12961 gagtgacgtg gtaatattcc ccgtgcattc tgtgatccgc aacattttgg gtcttaatga
    13021 ggaagaacgt cccaatccca atatggaggg taatctttag aacatttact ccggtctggc
    13081 gaatgacctt caatagctgt tctgtaaaat gaaaacctgc ggtcggcgct gctattgagc
    13141 caggcatttc tgcatagact gtctggtatc gatcaatatc tatagcaccg tcaccatctt
    13201 tccttttgac gtaaggcggc aggggcattt taccgtattc acccataata tcaaacattt
    13261 ctcggttttg atcaaactct atgatccatg actcaattcc acgtctaagt gtagcttccc
    13321 tgtggccaat caagacttta agttctctga ctcctccctt aacaccattt acaagacaaa
    13381 accagctttt atcattgatt ttctcgataa ggagaatatc aacgcgagcg ccagtcggtt
    13441 tttttgccga aatccgtgca ggaataacct tgctgtcatt taatacaagt acgtcactct
    13501 ctttcagata ttgggttacg tcacggaagg ccctgtgggt catcgatccg tcagccctgt
    13561 tgaatacaag catgcgcgaa gcttcacgct ctttttcagg atactgagcg ataaactcct
    13621 tcggcaggct ataatcaaat tcttcgatgt ttattcccat aaaaagtaag atatttttat
    13681 aacagattgc gcaattatta gcaaatcaat ttggacagtt acaaaggacg gcttttaata
    13741 ctcaatatat caacaagagc gcccggatag cttcaaatct ttctaaaaaa gctttacttt
    13801 ttctactatt agtcgaaata atatatatca atatggaaaa cttgcgggaa gaacttatag
    13861 ataaggcaaa aaccttgagg gtcttaccca cccttcacag tattattgaa aggttgtttg
    13921 taatccttga cgacaagaac tcctctttca gcgatctcaa cgatgtgata aaatacgatc
    13981 aggccatctc atcgaaaatt atcagtattg caaactctgc gtattacagc aggggcattg
    14041 aaatcttcag tcttcagagg gcgatgatca ccatgggctt cgaggaagtt aaaaacattg
    14101 ttatgtgcct tgttttcgta gaaaatgtcc tcaaagagtt gaaactcagg accgacatca
    14161 ttcttgaact ctggaagcat tctgtatttg ttgcatgtgc tgcaaggact cttgcagggc
    14221 gtatgttcag tgaagacccg caaaaggtgt tcaccattgc ccttcttcat gatataggca
    14281 agatcatctt ctatataact gttgaaaatt acaatgaatt gattgctaag gttcatagca
    14341 agggcaagtc cctgaacagg gaggagcgtg agatttacgg aactgatcac gaggagttag
    14401 gctatatcct gtcagttaaa tggcagttcc cagaagagtt ttcacagatt atccgttacc
    14461 atcatgatgg aaaggtggca ggtaaacacg aatccctctt aaggctcgtc agggctgcgg
    14521 acagattctc aacagcttcg gttcatgatt ctgaacctga aacatttatt ctgctgaaag
    14581 agagggacgg aataatgaag gaagtagaaa agataatgaa tatatttcaa ttgaatagcc
    14641 atggaacgag ataacttaaa atcagaatcg ataaactttc agtggaattt ttatgaacga
    14701 gctatcaaca actactctat cattatgaga atgctccact ttgtggagga agaggaaagc
    14761 cttgtcaaca acatgccccg tatattggtt gagggctcca ttttcgacat gtgcaaaatt
    14821 ttgcggaatg ataacgggat agagcgggaa ggttttttcg atatcgaaga atctctgaac
    14881 agtattgatt tgaatgcaat aaaatcgaaa tgtgccggtg tatttacacc gtctgtgctg
    14941 aaagatgcgt ttggttatgg aactctttat gtgtatccgc tcaaaaaaga tatggatatt
    15001 gtcggttttc tccttcttgg gcggagactc cccattgaat tggatgcaag aacgctccgt
    15061 gagcttgaga tagtctgcga catttacaat aaatcactta tcctccacat caacatccac
    15121 aatcataaaa aagcagcaaa gaggaaaagc atatttgaaa gcctcattaa tgagttccct
    15181 gatgcccttg ttatgattga caggaatggt tcaatagttt ttgccaacag aagggcaaag
    15241 agcgagttcg aaggggggaa aggctttctc gttggtgaaa agattgatag tgttatctca
    15301 ggcataggaa atgattttta ccggaaagag atggcgctcc acggggaagt caaatataaa
    15361 tcaggagata aattcaagat attcagggta gaaagctttc cattaaaaga agcagccgag
    15421 aaaggagtgt ggaaaggtct tctctttaag gatgtggttg aaaagaaaat taaagaagaa
    15481 gaatacgtcc gcaaggagaa aatggaaagt ataggaatgc ttgccggagg tattgcccat
    15541 gatttcaata accttcttac aggtgtgctc ggttatgctt ccctaataaa aaattttctt
    15601 tccaatgaag agaaactgta ccgatacgca gaggctatag agagctctgc tcaaagagct
    15661 gcaaaactcg cgcaacatct cctcaatttt tcaaggcggc agagaagggt ttctggagtt
    15721 gtggacatca atatgctcct tgaagacatc ctctttctca taaaggagag ttttcgtgat
    15781 atcgaaattg aaaagtctct tgatgccggt ctttatccga taaaaggtga tgagggagaa
    15841 ctccagaatg tattcctgaa tctttgtacg aatgcaaagg atgctatcac agggaagggc
    15901 aaattgaggg tacgtaccga acgaaaacgg tacgtaggcc accgtgagtt tgctctgata
    15961 gagatcgagg atacagggca gggcatagat gaagatatta aagaaaagat ctttgagccc
    16021 tattttacaa caaaggacaa tggcacaaac cttggcatgg gcctctacct cgttgataag
    16081 attatcaggg aacatggtgg ttttgtagaa ctacaaagtg agaaaaataa aggcacaaag
    16141 ttcagtattt atctgccact gccaacaaag atcgaggagc cagtgccggt aaaggaagag
    16201 cctgcgaatg acggattgac aaagaaacag acgatactgg ttgtagatga cgaagatgtg
    16261 gtgagagaac ttatcaaggg tgtgcttgct gatgagccaa cagatatcct tgaggcgcca
    16321 gatggtacac aagcaattga aatatttaag gataaatatg caagtatcga tcttgtaatc
    16381 ctcgatatga ttatgccagg aatcaaaggt gatgaggtct tgagggagat tcgctcaata
    16441 cgcaaagata tcagagttat catagcaagt ggttttatga gcgaagagca gcgtgatcgc
    16501 ctcaaacaat ataaggttga tgcttttctc gataagccct tccgtgacaa agatgttatt
    16561 caaacaatca gagacgtgct ctccaagaag taatatcggt ttccgttacg acgagtagtt
    16621 gaggttagga ttcagttctg gtatcgtatt agtgacggtc ctgtctttat tagtgtacat
    16681 atcatgcaga attttttcga attgacctta catcagcttg agaatgcaat cggcgccatc
    16741 gggaatgaaa aatttcgggc ccgacagctc tacaagtgga tttacaataa aggtgttttt
    16801 gatttccatg agatgaccaa catatcaaaa agccttcggc tgatttttaa ggacatgttt
    16861 tgtactgacc cacctgaaat caaagacatt ctccggtcca atgacggcac cattaagttt
    16921 ggtataacgg cttcggatgg acgtaccatg gaaagcgtcc tcattccgga aaagggcaga
    16981 aataccctgt gtttatcctc tcagattgga tgcaggatgg ggtgcaagtt ctgtgtgaca
    17041 gggaaaattg gtttcgtaag gaacctgaca tcagcagaaa ttatagggca gatcatggca
    17101 gtaaaaaggt atctggggga gcagaagatc accaacattg tatttatggg tatgggagag
    17161 ccggtagata accttgatac gctgctccac accctcgaaa tcatgaaaaa ccctcttggc
    17221 cttgattttt cacatcggag gataaccgtg tcttctgttg gccttttaga aggcctccga
    17281 acgctggagc caaaggttgc tggtcttgcc atatcactca acgcagcgga tgatgggaag
    17341 agaacctatc ttatgcccat caacagactt tacccaatac gggacattat cggttttgta
    17401 agggaattcc gaggcagcaa acgagtcaga ataacctttg agtatgttct gataaaaggc
    17461 ttcaatgatt cgctcgacga cgcgcaacaa ctggcgcagc ttctgacagg ggtaaaatgc
    17521 aagatcaact taatcccatt caatgagtct ccttatgtgg agttcaagac cccggatacc
    17581 aaagcagtca accagtttca tgactactta cttcaacgcc attttacagc cattgtgaga
    17641 gattcaaggg ggcaggatat tggagcagca tgcgggcagc tcggagcgag atacctggga
    17701 acattacagg aaataaactg aactggataa ctgattggct aatggaaagg gaattacaaa
    17761 aaaataatat gggctctaca aaagattaaa tgataatttt tcacgaaaat tgagagggaa
    17821 aaatggaaat aatgccagtg tacaaacaat cttttttctg taatcaagat agagaggatg
    17881 gtatgagact caacgtgatg tacgaagatc atactgttta ttgcgatata gttgttgata
    17941 accgatttga aggatatcac gatgttgttc atggtgggat gctttttggt atactcgatg
    18001 ttcttatgtg gcatatcatc ttcatggaga caaagaaagt cggtatgaca agaaagactg
    18061 aaatggaatt cttcaagcct gttatgtgta atgtacaata cagggcaatt ggaaagtttg
    18121 accgagttgt gggacgggat atatacgcct cagcgtggat agaggataga gaaggggaat
    18181 gttacgcgcg ggtcacagcc cttttcaggg aagggaaaaa tctgtctgct cccgatttct
    18241 taaaagactt cgatttcagt aaaacgaccc cagaaataaa ggcacgcttc atgtcactgt
    18301 tagaagatca gtaaaaagtg aaaagttaga agtaaaaatt gcaaaaaatt cataccacaa
    18361 aatctgactg aagcctttga tttttaaccg ttcaactttt cattcaccag acccatactt
    18421 gccagttcgg tctcgatggt ttgaaggagc ctttcaatgc caatcttttt aacactcgat
    18481 attgaaacag cccggtaacg ttcttcaaca tttctaagga atgatccatt tattttgtcg
    18541 atcttattga aaacgatgag gcgcctttta tccttcaatc ctaagggaac gagcaactct
    18601 tccacagttt caatcctgtc ttcaaaatct ggtgaactca catcaacaag gtgaagcagg
    18661 agatgggcgt catcaagttc ttccaaagtg gcaatgaagg cgcgaagcaa tacctgaggg
    18721 aggtcccgta taaaaccaac tgtatcggta agaataatgt tctttcgctc cgggtatttg
    18781 attttccttg ttgtagggtt tagggtactg aacggtttat cctctgcctc aaccttactt
    18841 tttgtgagaa gattcatcag ggtagacttg cctgaattgg tatatccaat aattgagaca
    18901 atggggatcc ctgagtacct tcgtttctct ctctttttgt cccttgccag ccttacttca
    18961 ttcagttttt tttctaagaa agatatttta tcccgaatcc ttctccgatc cacttcaagc
    19021 tttgtttcgc cggggccacg ggctcctata ccgccagtaa gtcgtgaaaa ggcgatgttg
    19081 gcttctgcga ggcgtggccg aatataacga agctgagcaa gctccacctg gatcttcgct
    19141 tcattggtcc ttgccctgcc ggcaaaaatg tcaaggataa gctgatttct gtcaatgacc
    19201 cgcaggtctg tcattgatga gatgttcttt atctgccctg ggctcagctc gtcatcaaag
    19261 acaaggaggt cgaccccaag ctgctggcat cggacaacaa tctcttctat ctttcccttt
    19321 ccaataagaa attttggatg gacctctttc ggcctctgta ttattgtttc caccactgtt
    19381 atgcccgcag aatagcaaag atctttcatt tccacaatgt gatcagccac atcctgtttc
    19441 cacctgggtg aagttaccga gacaagaata catcgctccc tatctttatc aaggacatag
    19501 tatctgcccc tttctttgac aaattcattt tcaagctctg tgatgaattc aatgaaatca
    19561 agatcgaatt cctggatagg gacaggcccg ataaagtccc atgcctgccc tttcttgttt
    19621 tgtggtatga ggtagcccac atgaatcacc agtccttcgt ccctcctctc agtgatgcag
    19681 gccaccaggt ctaactgcaa aagagcgagg tcagtgagat cctcctttga aagcaactcg
    19741 ccattgaggt gcgtgtggac aaatcttacc cctctgaacc gagcccttcc cacacgttca
    19801 gtagagggag ttggtacgcg tattccgttt ttatccccaa cgaccacgta ttctatcaaa
    19861 ccgcgcctat tgataataag gcctatttga cgggaaattt ctcttgatat ttgcaagatg
    19921 tttctggctg tgtcaaaggg gatgatacgg gaaggttcta cctttctttt atagaggttt
    19981 tcgagccgtc ttttgatgag atggttaagg ccctgagtat tcccgtatat ttctatatcg
    20041 ctttctccat tgcagcgtta ccgttatgcc ctatcaatga ggtccctatc aatttccatt
    20101 tttgcacaat aatgaacgcc ttcaaggaga atggccacgc gttctttacc atccagctca
    20161 ctctcaaaaa tgccgctgaa gcctttgaaa gggccgtcct taataagtat cttctctcct
    20221 ttatcgatct ttggtttctc aatggatgcc actccatctt caagtctcgc acggatgaaa
    20281 tcgatcattt catcttgaag gggcacaata gttcctccaa agtgaacgat ggttttgaca
    20341 ccgcgggcat acttgaccat tcggaactca tcaataggat ggaaccgaac aaaaatataa
    20401 ccaggaaaca taggttcaat gacctgaaca aactttcctt ccctgtactt tctcaacctt
    20461 agccgtggtg ccagaacctc tattccgcca tttacgaggt tcatggcagc cctgtcctca
    20521 tttctcggtt tcgtgttaac aacataccat cgtttcatgc atacttatta tctgcgatac
    20581 aggacaaatt atcaacaaaa acctcagatt gaccttcttg aaccaggcca ttcttctgga
    20641 atgacattat cctttctcaa attgcgagga tgtggtatat tttttacgaa gtcctgagaa
    20701 gttgctcaat ctcaactttt cacgttctca gcttctcatt gtatgctttt tttgcgcccg
    20761 tagctcagct ggatagagtg ttggcctccg gagccaaagg tcccgcgttc aaatcgcggc
    20821 gggcgcgctt ttaacatcag ataattagaa aactttcaca agcacaacag ctcccttgtt
    20881 cccttcgctg aagcgatttc cgagcaaagg acgacaagag atcccgtgaa accggctgaa
    20941 gtatcgatat ctgccaagtg gaagaaaaag ataaagttta ccctgatagg ctcccgaaac
    21001 agagccgtgc aactctcgtg gtcaacttga ggacctgctg ccggaatcga tgccgggtta
    21061 cagaagatcg aacggcccgg gccttcaacc ctattaccaa tcccacttcc cttcagaaga
    21121 aggcttccgg tcttcgtgcg gtgtagccag taacgaaaag cccctgttct caaatagcct
    21181 aatgatatca aatgactatt tcatggttgg gaagggaact tcagactaac atgcagtctg
    21241 attctgttat acattcatct ttggtcgaac aacggttgaa ccagagacag gaggatcttg
    21301 cactttcgct gattaactcc agtgactcgt tatcagtttt ttgcactata ccgaaaaccc
    21361 tatctctacc tttgagg
//
LOCUS       DAOOMC010000035        21350 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdus sp. isolate AMR_MDS_2685
            NODE_2413_length_21350_cov_6.094154, whole genome shotgun sequence.
ACCESSION   DAOOMC010000035 DAOOMC010000000
VERSION     DAOOMC010000035.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864760
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdus sp. (wastewater metagenome)
  ORGANISM  Syntrophorhabdus sp.
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae; Syntrophorhabdus.
REFERENCE   1  (bases 1 to 21350)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21350)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,729
            Genes (coding)                    :: 2,708
            CDSs (with protein)               :: 2,708
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 7 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21350
                     /organism="Syntrophorhabdus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2413_length_21350_cov_6.094154"
                     /isolate="AMR_MDS_2685"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2699756"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            889..1662
                     /gene="uppS"
                     /locus_tag="PK125_05135"
     CDS             889..1662
                     /gene="uppS"
                     /locus_tag="PK125_05135"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl diphosphate synthase"
                     /protein_id="HPB37523.1"
                     /translation="MPIKRLLYYSYGKLLEREVRKYRLPVHIGLILDGNRRYAKEMGF
                     EDVTMGHKAGAHKLDDVLKWCVELGIKIITIWVLSTDNTQRDEGEVAALLKVIEEKIN
                     DLSKNPIVRKNGIQIKALGNLDILPEELQRAIRKSELSTKDNRNHILNIALGYGGREE
                     IVDAVKKVIREKKTYSPEQLADNISVDDITSHLYTYGIPDPDLIIRTSGEIRLSGFLL
                     WQSAYSEFYFCDALWPVFRKIDFLRAIRSYQQRNRRFGR"
     gene            1659..3659
                     /locus_tag="PK125_05140"
     CDS             1659..3659
                     /locus_tag="PK125_05140"
                     /inference="COORDINATES: protein motif:HMM:NF012652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HPB37524.1"
                     /translation="MNTDADKISCLKSIELFKDLKEDELQKIAETLTEKVYPPNTVIL
                     REGATGDTMFFIKEGVVEVKKKEPYLGANLTVTILGKGECFGEMALLNENPRSATVLV
                     ATQPTVLLCIYKQAFNDLLVKYPSIAIHLNRVLAGRIEEANAQRGLDFVFISRLKLDP
                     NILLLIPEQVVASHKVLPISYSHNTLRLAMVNPNNLLAIDEVRKFVKGVAIEPVVMAE
                     EDLTRFMANEYPTIVGKESKETLFTRQVPFGGIDSLKADILKDIDLQEDLEGDAGVTD
                     LEQEAQAAPMIRLANTIIGMATKKEASDIHIEPMEKAVRIRFRIDGLLREEMILPKKV
                     QLPLMSRIKIMSRLDITERRLPQDGRTTLKVNDKSIDFRVSSIPTKCGEKIVTRILDK
                     TNTILGLEKLITDQDVLTLVREMIRRPYGIIYVTGPTGSGKSTTLYSALSELNSPDVN
                     ISTVEDPIEYDIAGINQVQVNPDIGLDFARVLRAFLRQDPDIILVGETRDTETAKIAV
                     EAALTGHLVFTTLHANDAPSAFMRLMEMGIEPFLISTSVIGVIAQRLVRKICPACKEP
                     FRADSTTTHFLQLPDNIELYRSVGCDTCGFTGYKGRIGVYEVLSVNDKIRHLVAEGSD
                     TQVIWEEAIKNGMKTLRDYCISLLQEGLTTVDEVLRTVAIQP"
     gene            3656..4459
                     /locus_tag="PK125_05145"
     CDS             3656..4459
                     /locus_tag="PK125_05145"
                     /inference="COORDINATES: protein motif:HMM:NF020893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HPB37525.1"
                     /translation="MNRNSIVKSRFLSICLRVLLYVFALLLIIEIFYLYHDGGWKELI
                     YYYRYFLNPKKLGEFIASFGPLAGLIFVVVQVIQVVAAPIPGEMTGFVGGFLFGKVWG
                     VILSTVGLTLGSMFAFWVARKFGIRLVERVVKKQYLDKFNFFVTHKGLYITFVMFLLP
                     GFPKDALCYFLGVTRLRFLDFILMNLFGRFPGTMILTMQGSAVRTEHWWELFWLLIIT
                     VVMIVTLYFSRNYVIELVRKITRSFIRSVIRRKKKKRSKAKSVIGKDVE"
     gene            complement(4391..5179)
                     /locus_tag="PK125_05150"
     CDS             complement(4391..5179)
                     /locus_tag="PK125_05150"
                     /inference="COORDINATES: protein motif:HMM:NF014703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HPB37526.1"
                     /translation="MTILESILLGALQGVTEFLPISSSAHLIILPWLFKIEDSTINKL
                     TYDVMLHFGTLLAILLIYGKQFVNICIEGLVDIWHKNFRQSVLLKIIVATIPAAVLGL
                     LFKDLIEGSLRTPYVTIFTLIGVSILMLVSERIPVENHALSYPIALLIGVAQAFALIP
                     GTSRSGITIVAGILLGLRRNAAVDFSFLLAIPIILGTSLFEARHVQFQGNGIDLYIAG
                     VISAFVFGVVSLKFLITYLKKHSLDIFAYYRFCLAAFLLLSSYY"
     gene            complement(5176..6000)
                     /locus_tag="PK125_05155"
     CDS             complement(5176..6000)
                     /locus_tag="PK125_05155"
                     /inference="COORDINATES: protein motif:HMM:NF024503.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HPB37527.1"
                     /translation="MMKEESWYKFLVISTIMHVFVMAAFSMSIKKPYKKFDLSSSYSV
                     GLVGDIGGGSGNKGVGLPQSKPIAEPKTPPEKLVSSPKEKKQLTAKHKPAPMKSDQDA
                     VSLSKKKTPPAKASPSKEELSQLEARLRDMKKRTEYLDVSQGRSAPGYERWGGGGGSG
                     SGSGGLPGSGEGGARPLDPASQQYMLGVWEKIKNAWGLPGMSKYSKNLETIVTIRIRK
                     DGRIVDINIEKRSGNRVYDESILRVLRSVDPLPPIPSSLNTDSLEIGFRFLPGELS"
     gene            complement(5997..6407)
                     /locus_tag="PK125_05160"
     CDS             complement(5997..6407)
                     /locus_tag="PK125_05160"
                     /inference="COORDINATES: protein motif:HMM:NF014525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ExbD/TolR family protein"
                     /protein_id="HPB37528.1"
                     /translation="MKSNRDSKSSPLSEINIIPLVDVMLVLLIIFMITAPMMQHGMNI
                     DIPKVTTKPLPTKDEPQILNITKDQRLILNEKKLAMKDLKAAIQLLFSNKHAKEIYLR
                     ADKEVPYGFVVSCMGLIREAGVEKINIVTKPREE"
     gene            complement(6404..7072)
                     /locus_tag="PK125_05165"
     CDS             complement(6404..7072)
                     /locus_tag="PK125_05165"
                     /inference="COORDINATES: protein motif:HMM:NF013760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HPB37529.1"
                     /translation="MFNVDLYTGGILGLLASAGPVAKFVVFILFLFSIISWTIILLKW
                     KQFSRTEKEGERFIKTVKDADSFKKIITSYRENPDNAFYRLIVSSYKEIASRQKENPG
                     PQTDVLPVVENTLRIVITEETEKLERRLSFLATTANTAPFIGLFGTVWGIMDSFREIG
                     VRGTTSLAVVAPGISEALIATALGLATAIPAVLAYNYFLGRLKRIISKMENASMYIVN
                     ILEK"
     gene            complement(7711..8925)
                     /locus_tag="PK125_05170"
     CDS             complement(7711..8925)
                     /locus_tag="PK125_05170"
                     /inference="COORDINATES: protein motif:HMM:NF002674.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tm-1-like ATP-binding domain-containing protein"
                     /protein_id="HPB37530.1"
                     /translation="MKKQLLIIATLDTKGREARYVRNCAIKLGVQPVVMDIGVIGKPF
                     IKPDITNRELTEAAGYDLEELIRSHNRPLAVIALKEGGRIVANHLLQKNQFNGVIGLG
                     GGTGTSVASSIMRSLPFGLPKVIVSTMASRDVREYVGTKDIVMFHSVADLLGFNEFIR
                     LILDQASRAVCGMMGRKISTKARKPIVGVTAYGPTSRCAVLTEKLLHEKGYEMMGFHT
                     NGCGGMAMEEMIAAGEIAGVLEFTPHEIADEMVGGYCKGIGPNRLETAGRMGIPLVFA
                     PGGLDNIVYGPSCPMPEDFSNRDIYAHDHRICVRLDTLEMEKLAKIIAEKLNKSPATT
                     HVLIPTGGWSEGDREGMPLFNPSANNAFTKRLRKLLNPKIPFEEMRLHINDLKFAQRA
                     VDILDTMMQINK"
     gene            9077..9976
                     /locus_tag="PK125_05175"
     CDS             9077..9976
                     /locus_tag="PK125_05175"
                     /inference="COORDINATES: protein motif:HMM:NF015431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HPB37531.1"
                     /translation="MILNMNQLRAFFVAAKSNSITKAADALMVTPPAITMQIKKLEKA
                     VGVRLLVRDGNSIRLTKTGDAVLTRAEKIFTEIHEMEGFFDDISMSKSGELRIGCPET
                     PLKRLAPLIADFNKTHPGIKIILDQGSNAEMVKSIGEHRNELAVIRCRSNYSRLKTKS
                     IWAEEVLLIAAPLSSHWSGPDISVMQLPVIPLILRREGSAVREIVLEYLRKFKVKPLI
                     AMESGSIVLVKEFVMQDRGLAFMEKGAVEEELKNGSLISVHILEGSPIIEFGIGYRNR
                     RDLSPPAWAFLRLLENSNITLRS"
     gene            complement(10259..10810)
                     /locus_tag="PK125_05180"
     CDS             complement(10259..10810)
                     /locus_tag="PK125_05180"
                     /inference="COORDINATES: protein motif:HMM:TIGR02175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate ferredoxin oxidoreductase subunit
                     gamma"
                     /protein_id="HPB37532.1"
                     /translation="MIEVRFHGRGGQGAVTSAELIAQAAIKQGLYAQAFPSFGPERRG
                     APVQAFLRVSDKPIRLRSKIYKPDHVIILDPTLLGTVNCADGLKPDGYIIINSHRSSD
                     ELKKLFPDHNIAAVDASTIAKEEMGVPITNTTMLGSLIKATSIVAIESLNEPIQNRFG
                     KIAQKNVNAYTRAHKETKIIKKE"
     gene            complement(10811..12268)
                     /gene="cysS"
                     /locus_tag="PK125_05185"
     CDS             complement(10811..12268)
                     /gene="cysS"
                     /locus_tag="PK125_05185"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR00435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HPB37533.1"
                     /translation="MGIRVYNTFSGKKEEFRPVNEGKVKLYVCGVTVYDHCHIGHARS
                     AIVFDVIYRYFMARGYEVVFVKNFTDIDDKIIRKSNSEGIPWKDVAEKYIVSFHEDMD
                     ALHVLRPTHEPKATEHIEDMIALVDNLINNGIAYKVNGDVYFSVENYKGYGELSGRTL
                     DEMMAGARVEIDEKKRNPLDFVLWKSSKEGEPKWPAPFGDGRPGWHIECSAMSAKYLG
                     LPFDIHGGGKDLVFPHHENERAQTESATGVKFANYWLHNGFVNIEKEKMSKSIGNILL
                     IKDFIEEFHPEVLRLFFLSTHYRNPIDYNKKSIEDTNQALHRLYYTLERVAEMEKQLP
                     VEPKPYGEAEELEKKYYEAMDDDFNTALALSYIFELSKMLNKMLDDDPTINAPFVLFT
                     NRLFLSLANTLGLLHDNLTDFTSTEKIRHLNRVGLTPEKLDRMIAERAEARKNKDYKT
                     GDQIRDVLARMGILLQDSPRGTEWRIKHITMKGEG"
     gene            complement(12336..12623)
                     /locus_tag="PK125_05190"
     CDS             complement(12336..12623)
                     /locus_tag="PK125_05190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB37534.1"
                     /translation="MKKYLIILLIVAGICYYYNPPLENHIESLSILAPEKLTEGDNFQ
                     AKIRENLDFINFYVASATKDRQRLSIVTFGCLGRVFVIDKEWLSWLSKGRP"
     gene            complement(13073..13762)
                     /locus_tag="PK125_05195"
     CDS             complement(13073..13762)
                     /locus_tag="PK125_05195"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPB37535.1"
                     /translation="MLDVKSINTFYGLSHILFDLSITVSKGEVVGLLGRNGAGKSTTM
                     RSIMGLTPPKEGSVIFKGEDVTGVKPFRLFRKGIGYVPDDRRVFADLSVDDNLEIVYK
                     QDTTWNKDRVYELFPALAEIKTRRAGHLSGGEQQMLTIARALMGSPELLLLDEPTEGL
                     APLIVRNLEEQILKLRDAGISILLSEQNVRSALKIITRAYVIDIGRIRFHGTVAELDA
                     NEEIKKKYLMV"
     gene            complement(13750..14499)
                     /locus_tag="PK125_05200"
     CDS             complement(13750..14499)
                     /locus_tag="PK125_05200"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPB37536.1"
                     /translation="MLQVKSLTKSFDGFKAVNNANLDVQNGEIVAVIGPNGAGKTTLF
                     NLISGILKPDSGQVLFKGDDITGLPPYEICRKRISRSFQVVNVFNRLSIFENTQISIL
                     SRQKKTWNLFTPSSSLAVNETNELLKNVGLYEKRDHISGALSHGDRKVLEIAMALGGN
                     PEFLILDEPTAGMSPEETTRCIDLIKGLREKYGLTILFCEHDMEIVFGIASRIMVMVR
                     GGTIVQGTCEEVRCNEAVQDAYLGGSDVCLM"
     gene            complement(14503..15459)
                     /locus_tag="PK125_05205"
     CDS             complement(14503..15459)
                     /locus_tag="PK125_05205"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HPB37537.1"
                     /translation="MATGILNIKGRIWWIVALVVLCLVPFVLPRFYVYLLSIILLFGL
                     FAMSNNFPLGFGGIYQLHHAVFYGVGAYGTALVITKSGLSPWIAFIVGPLIAAFLGLV
                     MGIICIRLSKLYFGMLQISLGSLVWAVVYRWYSFTGGDDGIHGISVPDIISSYKGAYY
                     FTLIVTGISLYVLYRLLKSPYGSVLQGIRDNPVRSSMIGVNVKKHQLLALVIAGFFAG
                     VAGTLFVVVDNTVFPDMMFWTLSLEAVIMCLLGGWLTFLGPMLGAALIVIIRTFVSTY
                     TLYWALVLGIIFTLVIFFLPNGVLGWIEDAVKRKPNLAEAKE"
     gene            complement(15473..16354)
                     /locus_tag="PK125_05210"
     CDS             complement(15473..16354)
                     /locus_tag="PK125_05210"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HPB37538.1"
                     /translation="MDVTGVLNQVFVGLSRTVILFIVSSGLSLILGVLRIPNVAHGSL
                     YMIGAFMAFTLSKLFGGGDMGFWMALIGAPLAVALISLVAERGIFQYLYEREHLMLIL
                     LTFAFSLVFGDLVKIVWGGEYKSVPVPYMFQGFIPLIGGLPFPLYNLFLLVCGPIIAI
                     LLWLLVNKTKIGKIARAAAVDMEMVGAVGINVSWVFASVFVIGCLLAGLGGVLVAPTV
                     SVTLGMDHTLIIEAFLIVIMGGLGNIWGALLGALIFGLSQSLGILIWPQFGIIFPYLA
                     VVIVLIFRPTGLLKATW"
     gene            complement(16388..17599)
                     /locus_tag="PK125_05215"
     CDS             complement(16388..17599)
                     /locus_tag="PK125_05215"
                     /inference="COORDINATES: protein motif:HMM:NF024825.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HPB37539.1"
                     /translation="MRRSVLFVVFMVFVMGLVWSQSGYSANTIKVGIVDTYSGPATTY
                     TQDALDGFKLATEQINAKGGILGKKLEYTTRDEKFKPDIGLAMAKELVLKEKVDILAG
                     TINSGTALAVSDFAKKEKMPFFVFYSKSEKIIGEKGHKYVFNMNENTEMAGRAAAVAL
                     SKKPYVKYWIAGDDYEYGHAIADATWNNLKKMKAGVQLLGQSWWKVGEADFTPYITQI
                     LAAKPDFIIVATGGSGMVNFQKAAKATGLVQKIPFYQHTANELGTLLPQGKDAPEGVW
                     GTSNYFFYYPETPANKAFVDDFRKAFNRYPKASALVGFMTAQFIAEGYKKAGKIDNEA
                     FIKAIEGMSLDSPVGPLAIRACDHQLELPTYWGVTKKDPKYDFLISGDIQVIAPKDYM
                     PTCQDVLKLRK"
     gene            complement(17821..18492)
                     /locus_tag="PK125_05220"
     CDS             complement(17821..18492)
                     /locus_tag="PK125_05220"
                     /inference="COORDINATES: protein motif:HMM:NF019349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HPB37540.1"
                     /translation="MARFIIKDNETIRKKVYHHIREKILNGEIAPGERLIETRLAHDI
                     GTSRTPVREALHSLELEKLIKSIPRVGYIVEGMDVHELEQICEIRSVIEALATRWAME
                     KSNKKLAKSLMKNVEKQEKALSKNRLQEYIELDAQFHDIIAKLSGSDRLLELDQTLRR
                     HMLRYRAHVVYAIDTAIRSVEGHRRIVNAIEEGNTNKAIEALQYHLQQSKEDILQYVL
                     SEHPQ"
     gene            18662..19672
                     /locus_tag="PK125_05225"
     CDS             18662..19672
                     /locus_tag="PK125_05225"
                     /inference="COORDINATES: protein motif:HMM:NF015445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005488 - binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DctP family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HPB37541.1"
                     /translation="MIKKMLSIVFVLAVFVALTSGVADAKTIVKYGHVGPPVHPQHHA
                     AVAFAKYVTEKTNGEIEVQVFPLGQLGGERSMTEQVQGGTLQMTSTTAGVLANFVPEM
                     GIIELPFVYPNRDVAYKVLDDKEVKDRFAKYCDAKGFVFIGYTENEFRDLTNSKRPIK
                     MPDDMKGLKIRVVESPMMIDTFRALGANPTPLPFPEIYNALQQKVIDGQDNPIYTSIL
                     MKFTEVNKFATITNHILTECPTIVNKAFWNSLKPEQQKIFREAAEVQAKVNREGNAKN
                     RVEALDKAKEQKVDVHILTDQERAAFKKAVQPVHDKYRAQYGADWYDFYLKKIDFYSK
                     KK"
     gene            19765..20271
                     /locus_tag="PK125_05230"
     CDS             19765..20271
                     /locus_tag="PK125_05230"
                     /inference="COORDINATES: protein motif:HMM:NF016200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HPB37542.1"
                     /translation="MENLIKTLNKVEEGLVGMSLLGLAILTFIETGLRYTVSYTFAWF
                     GEFANYMMIFCTYLGAAIGVKYGTHFSMEALTEYAPDRVSHLLKVFAYMMSGILCILF
                     VYYGIVHIVKLQSFGVKSSAMQLPMFIPYIPIPLFSIMMAFRFFVLSHKHFMSFLKRE
                     PFGRVRKK"
     gene            20275..>21350
                     /locus_tag="PK125_05235"
     CDS             20275..>21350
                     /locus_tag="PK125_05235"
                     /inference="COORDINATES: protein motif:HMM:NF018513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0015556 - C4-dicarboxylate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HPB37543.1"
                     /translation="MTLTIVLICFFILLTLSMPIALLLAATTAIYLIFIAHVPVTSLI
                     QQLFNGLDNFVLLGVPFFILAGNIMAEGAISDRLVGVMKLFVGRFTGGLALASVLACM
                     VFAAISGSSPATVIAIGSIMMPALIKEGYGNRFSIGLLTSSGSLGILIPPSIPMILYA
                     LVMNVSVAEIFMAGFLPGIFIGCSLMGYSYYMARKHGWRSQVHYSFKEGLKIIKDGLW
                     ALFLPVLVLGGIYGGIFTPTEAAAVSVVYALFVELFIYKELKIGKLFNICRDSAILSG
                     CLLFILSCAMSFIWLLTVEQIPIKLAEAIVSNIQNKWLFLLAINGVLLIIGALMDIVT
                     AIIVISPILVETLSRYDIDFIHYG"
BASE COUNT         5887 a         4663 c         4701 g         6099 t
ORIGIN      
        1 cttccgcctc gatatcctct atactctcct tgtgtgtgat ctgggaataa aggctttggt
       61 tctcctgtct gttcgaacat aagtggatca atatctatca tgatcttttt caaggtttgg
      121 gtggagattg ttcttgcccc ggtagaaaaa agcatggtat gtatgactgt ataagtcagt
      181 tgtacgtacc ccgtgctttc cgctgtcctt caaaaaataa ggactcattt cacgattaac
      241 gctgattgta ttatgactgt catgatagcc tcttttgatg gccatggttt atagttcaag
      301 tttttttcaa catcgtttta tcctcgttat gccattcagg acttctcgca ctttattcag
      361 gatttcgagg gtacggggtg tgttgtcggc atcggcactt ttataaaatt tgccaaacaa
      421 tcagaaacgc tatgcgcaat gattgtgtag atcctttaaa agggatgaca taatgtgctc
      481 ctacctatgc atggagagtt atgactcttg aagaggagct ccctttcaag gataatatta
      541 tccgtgccat gatgtgatgg ttggcactat caaccatttc aaaaactaca aaggtgtcta
      601 agcgaaaaaa cacacacttt gatgcctttc gcagactttt catttttgat atcaatcgtc
      661 aaaccttaat ggaaagttaa tatctcgtat ggagaggtca atatacgaat cagcccactt
      721 ctatagcccg gatgagttga ataatcttct tttggcaaaa ggattcaaga ttttgacaat
      781 aagcaaccta cggctctata agattgaagc tatgaaagag caatgaacgt gctttcctca
      841 aatatgtttg tgtattgagt tatattttca ttaacattat aatagtgaat gccaataaag
      901 cgtttgctct attattccta cgggaaactt ttggagcgag aggtaaggaa atacaggctc
      961 cctgttcata ttggattgat tctcgatgga aacagacgct atgccaaaga gatgggcttt
     1021 gaagatgtga ctatgggcca caaggcgggg gctcacaaac ttgatgatgt gctgaagtgg
     1081 tgcgttgaat taggcatcaa aatcatcact atatgggttc tctcaacaga caacacccag
     1141 agagatgaag gagaggtggc tgcgcttctt aaggtcattg aagaaaagat caatgacctt
     1201 tcaaaaaacc cgattgtcag gaaaaatgga atccagatca aggcactggg taatcttgat
     1261 atacttcccg aggagttgca aagagcaatc cgcaagtccg agctgtcgac aaaagacaat
     1321 aggaaccaca tcctcaacat agccctggga tatggtggcc gggaagaaat cgtcgatgca
     1381 gtaaagaaag taatacggga gaaaaaaaca tattctcctg aacaactggc tgacaatatc
     1441 agcgttgacg atattacaag ccatctatat acatatggaa tcccagaccc tgaccttatc
     1501 attcgtacaa gtggggagat tcgcttaagt gggttcctcc tctggcaaag cgcttacagc
     1561 gagttttatt tctgtgacgc cctctggcct gtttttagaa aaatcgattt tttgagggca
     1621 atccgtagct accagcaacg aaacaggaga ttcggccgat gaatactgac gccgataaaa
     1681 tctcctgcct caaaagcatt gaacttttca aagatctcaa ggaagatgaa ctgcaaaaaa
     1741 tcgcagaaac attaacagaa aaagtgtatc cacccaatac agttatcttg cgagagggcg
     1801 ccaccggtga caccatgttc tttattaaag agggtgtggt ggaagtaaaa aagaaggagc
     1861 cctaccttgg cgccaatctt acagtaacaa ttcttggcaa gggtgaatgc tttggtgaaa
     1921 tggctcttct taatgaaaac ccgagatccg caacagtgct cgtggccact caaccaacag
     1981 tgttattatg catatacaag caagctttca atgaccttct tgtaaaatac ccgtccatag
     2041 ctattcactt gaatagagtg cttgcagggc gaattgaaga agcgaatgct cagagggggc
     2101 ttgattttgt tttcatttcg agattgaaat tagatccgaa tatccttttg cttatccctg
     2161 agcaggtagt agccagccat aaagttcttc caatttcgta ctcccacaat accctcaggc
     2221 ttgccatggt gaacccaaat aaccttctcg caattgatga ggtcaggaaa tttgtcaagg
     2281 gagttgctat tgaaccggtt gtgatggccg aggaagacct cacaaggttt atggcgaatg
     2341 aatacccgac aattgttgga aaagaatcga aggaaacgct gtttaccaga caggttcctt
     2401 ttggaggaat tgactccctt aaggcagata ttctgaaaga cattgacctc caagaggatt
     2461 tagagggaga tgcaggcgta accgaccttg aacaggaggc acaggctgct cccatgatcc
     2521 gccttgccaa tacaatcata gggatggcca cgaagaaaga agccagtgat atccatatag
     2581 aacccatgga aaaggccgtg aggattcgtt tccggattga tgggttgctc agggaagaga
     2641 tgattctccc taaaaaggtg cagctccccc tcatgagcag gatcaaaata atgtcacggc
     2701 ttgatattac agagcgaaga ctgccacaag atggccggac aacgctgaaa gtgaatgata
     2761 aatctatcga ctttcgtgtc tcatccatac cgacaaaatg cggtgaaaag attgtcaccc
     2821 gtatccttga taaaacaaat accatcctcg gtctggaaaa actgattacc gatcaagacg
     2881 tcttgacact tgtacgggaa atgatcagaa gaccttacgg aatcatatat gtgactggcc
     2941 ctactgggtc cgggaaaagc acaacccttt atagtgctct ttcagagttg aacagcccgg
     3001 atgttaacat atcgactgtt gaggatccaa ttgaatatga tattgcgggt ataaatcaag
     3061 ttcaggtgaa cccagatata ggcctcgact ttgcaagggt tctgagggca tttctgaggc
     3121 aggatcctga tattattctt gtcggtgaaa caagagacac agagactgca aagattgcag
     3181 ttgaggctgc ccttaccgga catctggttt ttacaacact tcacgccaac gacgcacctt
     3241 ctgcattcat gcgtcttatg gagatgggga ttgaaccatt cctcatatca acatcggtaa
     3301 taggtgtcat tgcgcaaaga ctggttcgca aaatatgccc tgcatgcaaa gagcctttta
     3361 gggctgattc aacaacaacc cattttcttc aactacctga caatattgaa ctctatcgca
     3421 gcgtcggttg tgatacttgt ggttttaccg gatataaagg caggattgga gtgtatgaag
     3481 tcttgagcgt taatgataag atcaggcatc tggttgccga aggaagcgac acgcaggtaa
     3541 tttgggaaga agcaataaag aatgggatga aaacccttag agactattgc atttcactcc
     3601 ttcaggaggg tttaactaca gtcgatgagg tacttaggac tgtggcaatt caaccttgaa
     3661 tcgtaattca attgtcaaat ctcgattcct gtcaatatgc cttagggtct tgctctatgt
     3721 ctttgccctt cttcttatta tagagatttt ctatctttac cacgatgggg gatggaagga
     3781 acttatttat tactaccgct attttctcaa ccccaaaaaa cttggggaat ttattgcctc
     3841 ttttggccca ctggctggtt taatttttgt agtagtacag gttattcagg ttgttgcagc
     3901 gcctatccca ggggagatga ctggttttgt cggtggcttt cttttcggta aggtttgggg
     3961 ggtaattctc tcaacagtag gacttacatt gggatcaatg ttcgctttct gggtggctcg
     4021 gaaatttgga atacgattgg tagagagagt cgtaaaaaaa cagtacctcg ataaatttaa
     4081 tttttttgta acccataaag ggctctacat aacttttgta atgtttctct tgccaggttt
     4141 tcccaaggat gctttatgtt attttcttgg tgtcactcgt ttaagatttt tagatttcat
     4201 ccttatgaat ctttttgggc gatttccagg aacaatgatt ttgaccatgc agggaagtgc
     4261 ggtgaggact gagcactggt gggaactttt ctggctctta ataataaccg ttgttatgat
     4321 tgtaacgctg tatttctcga ggaattatgt gattgagttg gtaagaaaaa ttacccgttc
     4381 gtttatccgc tcagtaataa gacgaaagaa gaagaaacgc agcaaggcaa aatctgtaat
     4441 aggcaaagat gtcgagtgaa tgttttttca gataagttat gagaaatttt agactcacga
     4501 caccgaaaac aaatgccgat attactccgg caatgtaaag atcaataccg ttgccctgga
     4561 attgaacatg ccttgcctcg aacaacgaag tccccagaat gatcgggatt gcaaggagaa
     4621 aagaaaaatc gacagcagca tttcgcctca gtccaagaag aattcctgct actatagtaa
     4681 tcccacttcg tgatgtgcct ggtatcaggg caaatgcctg ggcaacaccg atcagcagtg
     4741 ctatggggta ggaaagcgcg tgattttcta ccggaattcg ttcgctcaca agcatgagga
     4801 tagaaacacc aatgagggta aagattgtta cgtaaggtgt tctcagtgaa ccctcgataa
     4861 gatctttgaa taaaagccct agaactgcag ctggtatggt tgccacaatg attttgagga
     4921 gaactgattg acgaaaattc ttgtgccata tgtcaactag accttcgata catatattta
     4981 caaattgttt tccgtaaatg agtaatatcg ccagaagggt gccaaagtgg agcatcacat
     5041 cgtaggttaa cttgttaatg gtgctatctt cgattttgaa gagccaagga aggataataa
     5101 ggtgtgctga gctgctgata ggcagaaact cagtaacacc ctgaagcgcg ccaaggagta
     5161 tgctttcaag aatggtcaag aaagctctcc tgggaggaac ctgaagccta tttcaaggga
     5221 atcagtatta agggaagaag ggatgggtgg caaaggatcg acagatctga gcactcgcag
     5281 gattgattca tcatataccc tgttaccaga tcgtttttct atgttgatgt caacaatacg
     5341 cccatctttt cgtatcctga tagtaacaat ggtctcaagg tttttactgt atttgctcat
     5401 accgggaagg ccccaggcat ttttgatttt ttcccagacc ccgagcatat attgctgaga
     5461 tgccggatca aggggtcttg ctcctccttc acctgagcca ggaagtcctc cgctaccact
     5521 tcctgagccg cctccgccac cccatcgttc atatccagga gctgatctac cttgagaaac
     5581 atcgagatat tctgtccttt ttttcatgtc gcgaagcctg gcctcaagct ggctcaattc
     5641 ttcctttgaa ggagatgctt ttgcaggggg agtcttcttt ttagacaggg aaaccgcatc
     5701 ctgatcgctc ttcatgggcg ctggcttatg ttttgctgtg agctgttttt tttctttggg
     5761 tgatgaaaca agcttttcag gaggcgtctt tggttctgct attggcttcg attgtgggag
     5821 cccaacgcct ttgttcccgc tgcctccacc gatatcaccc acaaggccaa ccgagtacga
     5881 ggaggatagg tcaaatttct tgtaaggttt tttgatagac atactgaacg ctgccattac
     5941 aaagacatgc attattgttg agataacaag aaatttatac cagctttctt ccttcatcat
     6001 tcttcacggg gttttgttac gatgttaatt ttctcaaccc cggcctcacg gatcaacccc
     6061 atgcaactta caacaaagcc ataaggaact tccttgtctg cgcgcagata aatttcttta
     6121 gcatgcttat ttgagaataa taactgaata gctgctttaa gatctttcat tgccaatttc
     6181 ttctcgttca gtataagtct ctggtctttg gtaatgttca gaatctgggg ctcgtctttt
     6241 gttggcagag gctttgtggt gactttgggg atatcaatat tcatcccgtg ctgcatcata
     6301 ggtgcagtga tcataaagat gataagaagg acgagcatga catcgacaag gggaatgata
     6361 tttatttcag agagggggct actcttagaa tccctgttcg atttcatttt tcaaggatgt
     6421 tgactatgta catagatgca ttctccattt tggagatgat cctttttagt ctgccaagaa
     6481 aatagttgta ggcaaggact gctggaatag ctgtggcaag gccgagagca gtggcaatga
     6541 gggcttcgct tatgccggga gcaacgacag caagactggt agttccccta acaccaatct
     6601 ccctgaacga gtccataatg ccccataccg taccaaaaag gcctatgaag ggggcagtat
     6661 tcgctgttgt agcaaggaaa gaaagtctgc gctccaattt ctctgtttct tctgtgatga
     6721 ctatcctaag agtgttttca acaacaggca gcacatcggt ttgaggcccc ggattctctt
     6781 tttgtcgtga tgctatttcc ttgtatgaag aaacgataag acggtaaaaa gcattatccg
     6841 ggttttccct gtatgaggta atgattttct tgaatgagtc ggcgtctttt actgttttga
     6901 tgaaacgttc accttctttt tcggtcctgc taaactgttt ccatttcagt agaataatgg
     6961 tccatgaaat tatggagaaa agaaaaagaa tgaaaaccac aaatttggca acaggcccag
     7021 cagatgccag aaggcccaat atgccgccgg tatatagatc aacgttgaac atgatttatt
     7081 gtgacgatta tacaatagga tggcgttgat ttcaatgaaa aagccaagac aggggatagc
     7141 tgtgaactga caataaagaa tgggattcaa ggaacaaagg gaataggact atctatcctt
     7201 gggatagaaa gaaaagcaag aacaatgtgc agtgacggtt gatcgggtaa tttggtctgt
     7261 tagcttgcat taaaataggc tgcttacctg atagccgggt aactaccttt atgtgattat
     7321 gttaagggtg gcctatagtc gatcaataca ctgtttacat cacataatac agaggagcga
     7381 tatgcctgac aaggcctcgg agaaggtgga tgtgagcacc tctattcaaa ataagaaacc
     7441 ccctgggggg gacccagggg gaaggggggt tattatgaga agcttatgga ggtgattttc
     7501 agttttttaa tcctgagata cttgtgaatt atattacatt ttcatgagct ctcaggcttc
     7561 ttgttatctt tcacaatccc ttttggctct gaatgccatg tacttgactc atatttagcc
     7621 tcggcattgt gaattattta acatatcgaa ttatagtatt ttgtagaaag cttgtcaaga
     7681 aaaaaatgta cactgtaccc caataaagtt ttatttattt atctgcatca tggtatctaa
     7741 aatatctact gccctctgtg caaacttcag atcatttatg tgaagcctca tctcttcaaa
     7801 gggaattttc ggattcagaa gttttctgag tctttttgtg aaggcgttgt ttgctgaagg
     7861 attaaagagg ggcatgccct ctctatcacc ttcagaccag ccgccagtag gtataaggac
     7921 atgggtagtc gcaggtgatt tattcaactt ttcggcaatt atctttgcaa gtttctccat
     7981 ttccaaagtg tcaaggcgaa cacatattct atggtcatga gcgtatatgt ctctattaga
     8041 aaaatcctct ggcatagggc acgaaggtcc atagacaatg ttgtccaaac cacctggtgc
     8101 aaaaacaaga gggatgccca tcctcccggc tgtctcaagg cggttcggcc cgatcccctt
     8161 gcagtagccc cccaccattt cgtccgctat ttcatgtggt gtaaattcta aaactcctgc
     8221 aatctcaccg gcagcaatca tctcctccat ggccatgcct ccacatccat tagtgtggaa
     8281 tcccatcatt tcatatccct tttcatggag gagtttttcg gtaaggactg cgcaccgaga
     8341 agttggcccg taggctgtta cccctactat gggttttcgt gcttttgtac ttattttcct
     8401 ccccatcatt ccacagacag cgcgtgatgc ctggtcaagg attagacgaa tgaattcatt
     8461 gaagcccagc agatctgcca cagaatggaa cataacaatg tctttggtac ccacatattc
     8521 tcttacgtcc cttgacgcca tagtggagac aatgaccttt gggaggccaa agggcaggga
     8581 gcgcataatt gatgatgcca cggaggttcc cgttcctcca ccgagcccga tgacaccatt
     8641 gaactgattt ttctgtagca aatgatttgc tactatgcgc ccgccttcct tgagggcaat
     8701 gacagccagt ggtcggttat gtgatcgtat cagttcctca agatcatacc ctgctgcttc
     8761 tgtaagttct cggtttgtta tatccggttt aatgaaaggc ttgccgatta ctcctatatc
     8821 cataacaacc ggttgtaccc cgagctttat ggcgcagttc ctgacatatc tggcttccct
     8881 gccttttgtg tcaagggttg caatgatcag gagctgtttc ttcatagctt cccttccccc
     8941 ttatgattag acaaacagtc cgcagagact ctggccgcgt ctgttccaat tttataatac
     9001 ttgtttgttg acaaatcaac tcaaataaga ttaattgcaa ttaagtaaaa ttaaatatct
     9061 aaaaggaaca atacatatga tcctgaacat gaaccagttg cgggcttttt ttgttgctgc
     9121 aaaatcgaac agtataacaa aggctgctga tgcgttgatg gtcactccac cagctattac
     9181 tatgcagata aagaaattag agaaagcagt aggcgtcagg cttcttgtcc gtgatggtaa
     9241 ctctatacgt ctgacaaaaa caggggatgc agtattgaca agggcagaaa aaatctttac
     9301 ggagatccat gaaatggaag gcttttttga tgacatatct atgagcaaat ccggagagct
     9361 ccgcatagga tgtcctgaaa cccccctgaa acgtttagca cctcttattg ctgactttaa
     9421 caaaacgcat cccggaatca agattattct tgatcaggga tcgaatgcag aaatggtcaa
     9481 aagcattggc gaacatcgaa atgagctcgc agttatcagg tgccggtcca actacagtag
     9541 gctaaaaaca aaatcaatct gggcagagga agtgcttctt attgctgcgc cattgagtag
     9601 ccattggtct ggtccagata tctcagttat gcaattgcct gtaatccctt taattcttag
     9661 gcgggaaggt tctgcggtca gagagattgt attggaatat ctccgtaaat tcaaagtaaa
     9721 acctctcata gccatggaat ccggaagcat agtccttgtt aaagagtttg tgatgcagga
     9781 caggggatta gcatttatgg agaaaggcgc agtggaggaa gagctaaaga atggctcctt
     9841 gatatcagtg catatccttg aaggctctcc tataatagaa tttggcatag gatatcgaaa
     9901 ccgtcgggac ctatcaccgc cagcttgggc cttcctcaga cttcttgaaa attccaacat
     9961 tacgttgagg tcttaagagg acggcctgtt acgtttggcc cagatgaatg aaataaaact
    10021 gttgtccacg gaagggggtt ttgtcagtaa gcgttagtat tgaaatgact ggcacaatcc
    10081 tataacacag ggccatgaaa ggtggctggg atgaagacat acggtctatc cttagcacca
    10141 catacccagc gtgttacgag aagccttggt ggagagaaaa tatgtgaatg gctatagacc
    10201 atgagcaata agcaacatct gaaaagtcaa tctatagccc tttacctctc gcaagggttt
    10261 actccttttt tattatcttt gtttctttgt gtgctcttgt gtaagcgttc acatttttct
    10321 gtgcgatttt accaaatcta ttctgaattg gctcgttgag ggattcaata gcaacgatgc
    10381 ttgttgcctt gatgagagag ccaagcattg tagtgtttgt tatggggacg cccatctctt
    10441 cttttgcaat cgttgaagca tccacagcag cgatgttgtg atctgggaag agttttttta
    10501 gctcatcaga ggatctatgg gaattaatta tgatataacc atccggtttc aggccgtcag
    10561 cgcagttgac cgtaccaagc aacgtggggt caaggatgat tacatggtca ggcttgtaaa
    10621 tttttgatct tagccgtatg ggtttatcag acactcttaa aaatgcctgg accggtgctc
    10681 ctcttctttc cggtccaaag cttggaaatg cctgtgcata taagccttgt tttatggcag
    10741 cctgagcgat gagttctgct gatgttacgg ctccctgtcc tcctcttcca tgaaatctaa
    10801 cctctatcat ctaaccttct cctttcatag ttatatgttt tatccgccac tcagtgcctc
    10861 tgggcgagtc ctgaagaagt atgcccattc ttgcaagaac atcccgtatc tgatctccag
    10921 tcttatagtc tttgtttttc ctcgcctctg ccctttcggc tatcatcctg tccagttttt
    10981 ccggagttaa tcctactcgg ttgaggtggc gtattttttc tgtggaagtg aagtccgtca
    11041 aattgtcatg caacaggcca agagtgtttg caagagaaag aaaaagcctg ttggtgaaaa
    11101 ggacgaacgg agcatttatt gttggatcat cgtcaagcat cttgttgagc atttttgaca
    11161 gttcaaagat gtaagatagg gccagagcag tgttaaaatc gtcatccatg gcctcatagt
    11221 attttttctc cagctcctct gcttcaccgt atggcttcgg ttccacaggg agttgttttt
    11281 ccatttcagc caccctctca agggtataat agagcctatg gagtgcctgg tttgtatcct
    11341 caattgattt cttattatag tcaatgggat ttctgtaatg ggttgagaga aagaaaagcc
    11401 tcagtacctc agggtgaaac tcttcaataa aatctttaat gagtagtatg tttccgatgg
    11461 atttggacat tttttctttt tctatgttta caaatccatt atgaagccag taattggcaa
    11521 acttcacacc ggtggcagac tcggtttgcg cccgttcatt ctcatggtga gggaagacaa
    11581 ggtctttacc acctccgtgt atgtcaaaag gcaaccccaa atattttgcg ctcatagcag
    11641 aacattcaat gtgccatcca ggcctcccgt ccccaaaagg agcgggccat tttggttccc
    11701 cttcctttga agatttccaa agcacgaaat cgaggggatt cctctttttc tcgtcaatct
    11761 caacgcgggc accagccatc atctcatcca gggttcttcc cgacagttca ccatagcctt
    11821 tatagttctc cacagagaag tagacatctc cgtttacctt atacgcaatc ccgttattga
    11881 tcagattgtc aaccagggct atcatatctt caatgtgttc cgtggccttt ggctcatggg
    11941 ttgggcggag aacatgaagg gcatccatgt cttcatgaaa ggatactatg tatttctctg
    12001 ccacgtcttt ccacggaatg ccttcgctgt ttgacttcct tatgatcttg tcatcgatat
    12061 cagtgaagtt ctttacaaag acaacctcat aaccccttgc cataaagtat cgataaataa
    12121 catcaaagac gatagcgctt cgggcatgac caatgtggca gtgatcataa actgtgacac
    12181 cacacacata gagttttacc ttgccttcgt ttactggcct gaattcttct tttttacccg
    12241 aaaaggtatt gtagactctg atgcccatga atctgtatat ttatcatatg gtttataggc
    12301 ttgtcaaaga ttacttggcc aattccaggc ctattctatg gacgaccctt tgaaagccac
    12361 gaaagccatt ccttatcgat aacaaaaacc cttccaagac aaccaaaggt gacaatagaa
    12421 agcctttgcc tatcttttgt tgcagaggct acatagaagt ttataaaatc cagattttct
    12481 cgaatctttg cctgaaaatt atcaccttcg gtcagttttt caggggccag aatgcttaaa
    12541 gactctatgt ggttttcaag aggaggatta taatagtaac aaatccctgc aacgatcaag
    12601 agaataatca ggtatttttt cataagcccc ccttggatgg aacgattata tggaatattg
    12661 agcacaacat aactaaagtt tatacactaa ttcagttgtg caggcaatca tttaaaaata
    12721 tatggcaggt agggatcctg cttaatcgga acacgaaagg gactcagaac gaagggcgat
    12781 tagtgatcag tatgttctca tcatgactac ttgtgggact ccaattttgc gggggagcga
    12841 ggttttctga tatcctcaca tgatcttatt ttgcgttttt gccgaaaaag gagtgatggt
    12901 gtatcagtga atttgtctga aagcatagca aatacagggg atgcaacgaa aatgctaaca
    12961 ataataaaaa gctgctcggt tatgggcggg accctttatg tgaccttttg attgataaca
    13021 gcgaccactc aagaggggga ggaacatcac atattttccc ctggtcagcg cgttatacca
    13081 tcaggtattt cttcttaatt tcctcatttg catcgagttc tgcaacagtt ccatgaaagc
    13141 gtattcgtcc gatatcgatg acgtaagccc tagttattat cttgagggct gacctgacgt
    13201 tctgttcgga gaggagaatg ctgatacctg catctctcag tttaagtatc tgttcttcaa
    13261 ggttgcgaac aattaaaggt gcaagtcctt ccgtaggttc gtcaagcaga agaagttcag
    13321 ggctgcccat gagcgcacgg gcaattgtga gcatctgttg ttcaccgcca ctcaaatgac
    13381 ctgccctccg tgtttttatc tctgcgaggg caggaaatag ttcatagacc ctgtccttgt
    13441 tccaggtggt gtcctgttta taaacaatct ccaggttatc gtcaaccgaa agatcggcaa
    13501 aaactctccg atcgtcaggg acatagccaa tgcctttccg gaacaatcgg aagggcttaa
    13561 cacctgtaac atcctctcct ttaaaaataa ctgagccttc cttaggaggg gtgagcccca
    13621 taatactccg catggtcgtg ctttttccag ccccattcct gccaaggagt cctaccacct
    13681 cccctttgga cacagttata gacaagtcaa aaaggatatg gctcaatcca taaaatgtat
    13741 tgatagactt tacatcaagc atacgtcact cccgcccagg taagcatctt gaacagcttc
    13801 gttgcaccga acctcttcgc aggtaccctg tacaatcgtt ccgcctcgaa ccatcaccat
    13861 gatccggctt gcaataccaa agacaatttc catgtcgtgc tcacagaaca gaatggtaag
    13921 cccatatttt tctctcaagc ccttgatgag atcgatgcag cgggttgttt cttcaggaga
    13981 cattcccgct gtaggttcat caaggataag aaattccggg tttccaccta atgccatggc
    14041 aatctcaaga acttttctgt cgccatggga aagagctccg cttatatggt ctcttttttc
    14101 gtagagacca acgttcttca gaagttcgtt tgtttcgttg acagcaagac ttgaggaagg
    14161 agtaaaaaga ttccatgtct ttttttgtct cgaaaggatc gatatctgag tattctcaaa
    14221 aatactaagc ctgttgaata cgttgacaac ctgaaacgac ctcgatattc ttttcctgca
    14281 tatctcgtag ggggggagtc cggtgatatc gtcacccttg aaaagcacct gcccggaatc
    14341 cggctttaaa ataccggaaa taaggttaaa gagcgttgtc tttccagccc catttggacc
    14401 gattactgct acaatctcgc cattctgaac atcgagatta gcattgttta ccgctttaaa
    14461 accgtcaaaa gattttgtta aggactttac ttgtaacacc ggttactcct ttgcttctgc
    14521 aaggtttggt tttcgcttca ccgcatcttc tatccatccc agcacaccat tcggcaggaa
    14581 gaatatgaca agtgtaaaaa ttatgccaag aacaagagcc caatacaatg tataggtgct
    14641 cacgaaagtc ctgataatga caatgagtgc agcgcccagc atgggtccaa gaaaggtcag
    14701 ccagcctccg agcaagcaca tgatgactgc ttccaacgat agagtccaga acatcatatc
    14761 aggaaatacc gtgttatcga caacaacaaa gagagtgccg gccactccgg caaaaaaacc
    14821 tgcaattaca agagcaagaa gctggtgctt cttaacgtta actcctatca tagagcttcg
    14881 aaccgggttg tcccggattc cctggagaac acttccataa ggagatttaa ggagtctgta
    14941 aagaacatag agggaaatgc cggtgactat gagggtaaaa tagtaggccc ctttgtaaga
    15001 agatataatg tcaggtactg aaatcccatg gattccatca tcacctccgg tgaaagagta
    15061 ccatcggtac acgactgccc agacgaggga tccaagagaa atctgaagca taccaaaata
    15121 aagctttgag agcctgatgc agatgatgcc cataacaaga cctaaaaatg cagctatgag
    15181 aggtcccacg ataaatgcga tccagggcga gaggccagat tttgtaataa caagggcagt
    15241 cccgtaagcc cctacgccat aaaatacagc atggtggagc tggtagatac cgccaaaacc
    15301 aaggggaaaa ttgttgctca tagcaaaaag cccaaagagc aagatgattg ataagagata
    15361 aacatagaat cgtggcagca caaaaggtac cagacagagg acaacgagcg ccacgatcca
    15421 ccagattctg ccttttatgt tcaatatccc tgttgccata ttatgctcct cgttaccatg
    15481 tagctttcag cagacctgtg ggcctgaaaa taagtacgat gactacagcc aggtaaggaa
    15541 aaataatgcc gaactgtggc caaataagaa tacccagaga ctgtgaaagt ccaaatataa
    15601 gcgcgccgag aagagcaccc catatattgc ccaggccgcc cataataact atcagaaatg
    15661 cttctataat caaggtgtgg tccataccaa gggtaacgct gacagttgga gctacaagta
    15721 cgccaccaag accggcgagc agacagccaa tgacaaagac gctggcaaaa acccagctca
    15781 catttatacc tacagcaccc accatctcca tgtccacagc agcggcccta gcaatttttc
    15841 caatcttggt cttattcacg agaagccaga ggaggattgc tatgatcggg ccacacacaa
    15901 gaagaaaaag gttgtagagg gggaaaggca gaccgccaat aagggggata aacccctgaa
    15961 acatgtatgg gacgggtact gatttgtatt ccccgcccca tactatttta acgagatctc
    16021 caaagaccag cgaaaatgcg aaggtcaaaa ggattaacat aaggtgttcc ctttcgtaga
    16081 ggtattggaa aataccccgc tctgcgacaa ggcttatgag tgcaacagcg agcggggcac
    16141 cgatgagggc catccagaat cccatgtccc ctccaccaaa aagtttagat aaggtaaagg
    16201 ccataaaggc gccaatcatg tatagggaac catgtgctac atttgggatc cgcaaaaccc
    16261 ccaggataag actcaaccct gaagagacta tgaagagaat tactgtacgg ctcaagccca
    16321 cgaacacctg attcaatacc cctgtcacat ccacagcctg atctccttgg ttatattgac
    16381 atattaacta ctttcggagc tttaaaacat cctggcacgt tggcatataa tctttgggag
    16441 caatgacctg gatatcaccg gagattaaga aatcatactt cgggtccttt ttggttacgc
    16501 cccagtaagt gggcagttca agttgatgat cgcatgccct gattgcaaga ggccccaccg
    16561 ggctgtcaag gctcattcct tcaatggctt ttataaatgc ttcattatcg attttgcctg
    16621 cctttttgta accttccgcg ataaactggg ctgtcataaa gccaaccagt gcgcttgctt
    16681 ttggatacct gttaaatgcc tttctgaaat catcaacaaa ggccttattt gctggagttt
    16741 caggatagta gaaaaagtag ttagaggttc cccatactcc ttcaggcgca tctttgccct
    16801 gcggaaggag ggttccaagt tcgtttgctg tatgctgata aaaaggaatt ttctgaacga
    16861 gaccagtggc cttggcggcc ttctggaagt tcaccatgcc tgaaccaccg gtagcaacga
    16921 taatgaaatc aggttttgca gcaagaatct gggtaatata aggggtgaaa tccgcctcgc
    16981 caactttcca ccaggactga ccgaggagct gaacacctgc tttcattttt ttcaggttgt
    17041 tccacgttgc atcagcaata gcatgaccgt attcgtagtc atcgcctgca atccagtact
    17101 tcacgtaggg ttttttggat aaggcaactg cagcagccct ccctgccatt tccgtgttct
    17161 cattcatatt aaagacatat ttgtgtcctt tctcacctat gatcttttca ctcttagaat
    17221 agaaaacgaa aaaaggcatt ttttcttttt tcgcaaaatc tgaaactgcc aatgctgtcc
    17281 cgctgtttat ggtacctgcc agaatgtcaa ccttttcctt gaggacaagt tcttttgcca
    17341 tggcaagacc gatatccggt ttgaatttct catcacgagt ggtatactcc agcttttttc
    17401 caagtatacc gccttttgca ttgatctgtt cagtagcaag cttgaatcca tcaagggcgt
    17461 cctgggtata cgtagttgca ggtcctgaat aggtatcaac aataccaacc tttattgtgt
    17521 ttgctgaata acctgactgc gaccagacaa gacccatgac aaaaaccata aacactacaa
    17581 acaaaactga tcttcgcatc ctgtacctcc ttgattagtt tctggggaat ctttccagcc
    17641 cccctcaatg attgataaat gttactatgt atcttttcat actgtcaatc aaagaaatat
    17701 gattttctcg gtcatgtggc aagaaaaaat actggagaaa aagcacatga tatggaggtt
    17761 acagcggctg ttccatagag ctttaatttt tcagataatg cttgatggct gagcaattac
    17821 tcactgaggg tgttcgctta aaacgtactg aagaatatct tcttttgact gttgaaggtg
    17881 gtactgaagt gcctcaattg ccttattagt attcccctct tcaatggcat tcacgatacg
    17941 acgatgcccc tccacggatc gaatagcagt atcgattgcg tagacaacat gagcccggta
    18001 ccgtaacatg tgtctccgca acgtctgatc cagttcaaga aggcgatcac tgccactcag
    18061 ttttgctata atgtcgtgaa attgtgcatc gagttcaatg tactcctgca atctgttttt
    18121 gctcagggcc ttttcctgct tttcaacatt tttcatgaga ctttttgcaa gttttttatt
    18181 agacttttcc attgcccaac gagttgcaag tgcctcaatg actgatctga tctcacagat
    18241 ttgctccaac tcatgcacat ccattccttc aacaatataa ccgaccctgg gtattgactt
    18301 aatcagtttt tcaagttcaa gactgtgtaa cgcctcacgg acaggggttc gcgaggtacc
    18361 aatatcgtga gcaagccgtg tttctataag acgttcgcca ggcgcaattt ctccgtttaa
    18421 gattttttct ctaatatgat gatagacttt ttttctgatt gtctcgttat ctttaatgat
    18481 gaatctggcc attcattgtg tacagtaccc cagatatctg attgatgtca acagattttg
    18541 ttgaggcttc tacaatcaga acgaatgaag caaggatttt ttatttgaca agacaggttt
    18601 tttgtataag aattcttttg ataaagtcta tattaaaaca atatcaaaca agggggtcat
    18661 catgattaaa aaaatgctgt ccattgtttt tgttttagcg gtttttgtgg ctttaacttc
    18721 tggcgttgca gatgccaaaa cgattgtgaa gtatgggcat gttggcccac ctgtccaccc
    18781 tcagcaccat gcagctgttg ctttcgcgaa gtacgtaact gaaaaaacaa acggagaaat
    18841 tgaagttcag gtatttcccc ttggccagct aggaggcgag cgctcaatga ctgagcaagt
    18901 gcagggtggg acactccaga tgacctcaac aacagccggt gttctcgcca actttgttcc
    18961 cgagatgggc atcattgagc tgccttttgt ttatccaaac agagatgtgg catacaaggt
    19021 tctcgatgat aaagaagtga aagatcggtt tgcaaaatat tgcgacgcaa agggttttgt
    19081 ttttatcggt tacaccgaga atgagttcag ggatttaacc aactcaaagc ggcccataaa
    19141 aatgcccgat gacatgaaag gtttgaaaat aagggttgtt gagtctccca tgatgattga
    19201 cacgttcagg gctcttggtg ccaatcccac accactgcct tttcctgaga tttataatgc
    19261 actgcagcaa aaggtgattg atggccagga caacccaatc tatacctcca tccttatgaa
    19321 gtttactgaa gtaaacaaat ttgccaccat cacaaaccac atcctcaccg aatgcccgac
    19381 aattgtgaac aaggccttct ggaattccct taagccggaa cagcaaaaga tcttcaggga
    19441 agcggccgaa gttcaggcaa aggttaacag agaaggaaac gccaagaaca gggtagaagc
    19501 acttgacaaa gcgaaggaac aaaaggtgga tgtgcatatc cttactgacc aggaaagggc
    19561 tgcctttaaa aaagctgttc agcctgtgca tgacaaatat cgtgcccaat atggggctga
    19621 ttggtatgac ttttacctga agaagatcga cttctattcc aagaagaagt aaaaaacaga
    19681 aagtatgttg attaggctgg atggattgag tttgccagac catttagcct aatcaactga
    19741 ttcaacgtaa tatactgctt tcagatggaa aacctgataa agacactgaa caaggtcgag
    19801 gaggggcttg tcggcatgtc actccttggc ctggcaatcc tcacatttat tgagaccgga
    19861 cttcgatata ctgtttccta tacctttgcc tggtttggtg aattcgccaa ttacatgatg
    19921 atattctgta cctatcttgg agctgccata ggagttaaat atggtactca tttctccatg
    19981 gaggcgctta ccgaatatgc ccctgacagg gtgagccacc tcttgaaggt cttcgcatat
    20041 atgatgtcgg gcatcctgtg tattcttttc gtatattacg gtattgtcca cattgttaaa
    20101 ctccaatcat tcggtgtcaa aagctctgct atgcagctgc ccatgtttat cccctatatc
    20161 cctattcctc tcttctctat tatgatggcc ttcagatttt ttgtactttc ccataagcat
    20221 ttcatgagtt ttctgaagag agagcccttt gggcgggtaa ggaagaaata accaatgacc
    20281 ctaacgattg tcctcatctg tttttttatt ctccttaccc tgagcatgcc cattgcctta
    20341 cttcttgccg ccacaactgc tatttacctg atctttatcg cccatgtccc tgttacatcc
    20401 cttatccagc agctctttaa tgggctcgat aattttgtgc tcctgggggt gccctttttc
    20461 atacttgccg gtaacattat ggcagaaggg gctatctctg accgcttggt tggggttatg
    20521 aagcttttcg tagggaggtt caccggaggt cttgcccttg cgtctgtttt ggcgtgcatg
    20581 gtctttgcgg ccatctctgg atcgtccccg gcaacagtta ttgcaatcgg gagcatcatg
    20641 atgcctgccc tcatcaagga aggttatggt aatagattct ctatagggtt gcttacatcg
    20701 tccggttctc taggcatcct catcccgccc agtatcccca tgattctcta tgcccttgtc
    20761 atgaatgtct ctgtggcaga aatctttatg gcaggatttc ttcccggcat attcatagga
    20821 tgtagcctta tgggctactc gtactatatg gctcgcaaac atggatggcg gtcacaggtc
    20881 cactattctt ttaaagaggg tttgaaaatt atcaaagatg gcctgtgggc actctttctt
    20941 cctgtgcttg ttcttggcgg aatatacggg ggaatcttca ctcccacaga agctgctgca
    21001 gtatcagtag tttatgcttt atttgttgag cttttcattt acaaagagtt gaagattggc
    21061 aagctattca atatatgcag ggactctgct atcctgtcgg ggtgcctact ctttattctt
    21121 tcctgcgcca tgagttttat ctggcttctg actgttgaac agattcccat taagctggcc
    21181 gaagcaatcg tttcaaacat tcagaacaag tggctctttc tcctggccat caatggggtc
    21241 cttctcatta tcggcgctct tatggatatt gtgacagcca ttattgtcat ctcaccaatc
    21301 cttgtggaaa cgctcagcag atatgatatt gatttcatcc attacggtat
//
LOCUS       DAOOMC010000036        21258 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdus sp. isolate AMR_MDS_2685
            NODE_2439_length_21258_cov_6.731689, whole genome shotgun sequence.
ACCESSION   DAOOMC010000036 DAOOMC010000000
VERSION     DAOOMC010000036.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864760
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdus sp. (wastewater metagenome)
  ORGANISM  Syntrophorhabdus sp.
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae; Syntrophorhabdus.
REFERENCE   1  (bases 1 to 21258)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21258)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,729
            Genes (coding)                    :: 2,708
            CDSs (with protein)               :: 2,708
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 7 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21258
                     /organism="Syntrophorhabdus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2439_length_21258_cov_6.731689"
                     /isolate="AMR_MDS_2685"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2699756"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..697)
                     /locus_tag="PK125_05240"
     CDS             complement(<1..697)
                     /locus_tag="PK125_05240"
                     /inference="COORDINATES: protein motif:HMM:NF014757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HPB37544.1"
                     /translation="MAIKTIGVLGAGVMGNGIAQVASMAGYNVIMRDIEDRFVESGIK
                     NIDKFLTKSVEKGKMTADAKKEVMGRIKGTTAMADLKDVDFVVEVIIEVMDVKKKVFA
                     ELDELTRKDVILSSNTSSMSITEIATATKRPDKVVGMHFFNPVPLMRLVEVVRGYNTS
                     DETVAITIDLTKKLGKEPVEVKKDVPAFIVNRLMIPHFIEAMKLLEQGVATKEDIDKA
                     AKLGLNYPMGPFEL"
     gene            complement(957..2006)
                     /locus_tag="PK125_05245"
     CDS             complement(957..2006)
                     /locus_tag="PK125_05245"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HPB37545.1"
                     /translation="MACQLLFSQLQKLLAITHLYNHKKLEAHKVTIYYIIIIIQTFQE
                     VFLIFNKTGQVKEIFFVAGLAWSPVYVVAAEEPVLFEAGFHCAGRLYEEDIRDFLNGR
                     NPKTLFLTHVHWDHCGSTAYLKKCFPGLKVAASRISAEIIRRPNAQNLMKRLSQDVIP
                     LVGQVHGVDKEKLIRDSFEPFEIDIIVDGGQTLNIGGGLTLQVIATPGHTRDMLSYYI
                     PEKRVLFATESAGVLDQADQIIPEFLVDYDLYIASLKELKKLDIDVLCQGHHFVFVGD
                     DVKRFFDRSLEAAKKFRDDVQDLLISEGGSIERVVHRIKARQYDTNRGVKQSEKSYLL
                     NLTARVTHLARKLQN"
     gene            2069..3226
                     /locus_tag="PK125_05250"
     CDS             2069..3226
                     /locus_tag="PK125_05250"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HPB37546.1"
                     /translation="MDFKISDELESMRKMAYDFAVKNIKPTMEEDEKEHKFRPEIMKK
                     MGDQGMFACIAPEKFGGLALPEGHMAATLMAMEVARISPSWGLPFNLQMNGPQNALMK
                     FGSEELQEQYLPGLIAGTSGGCFAITEPNSGSDVASMKTTATEVDDGFILNGTKTWIS
                     GVPFCDCGIVYAMTDKAAKHKGMSAFFVDFKTPGIVQRAITTKLGLFCAPTGEIFFEE
                     AKIPKSALVGKLGQGFAICMTMLNFTRLSSAARAVGVAQSCIEEACKYANEREQFGQP
                     IGQFQMVQEQIGRMSVEHEAAKLLLLRAAWQKDQGANNTLETSMAKYYCAESANFCAS
                     EAVKIFGSYGFSSEYPVERYLRDAKSYQIVEGTSNVQKMIIAQFALGYRKA"
     gene            3255..3470
                     /locus_tag="PK125_05255"
     CDS             3255..3470
                     /locus_tag="PK125_05255"
                     /inference="COORDINATES: protein motif:HMM:NF012584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HPB37547.1"
                     /translation="MATEVTVPMVGKIVGVNVNVGDKVNEDDQIATLEAMKMEMPIVS
                     PVSGTVKEIKVAAGQEVEADAVLAIIE"
     gene            3494..4273
                     /locus_tag="PK125_05260"
     CDS             3494..4273
                     /locus_tag="PK125_05260"
                     /inference="COORDINATES: protein motif:HMM:TIGR00241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008047 - enzyme activator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydratase activase"
                     /protein_id="HPB37548.1"
                     /translation="MLVAGIDIGSITTEALLFDKDKGIVGYTILQTGADSKKTAEIAL
                     EKVLSYPGKSISDVSYVISTGCGRKRAAFAKEAVTEITCIAKGVNYLFPEARTIIDIG
                     GQDTKVVRIDEKGRVLEFEMNDKCAAGTGRFIEVMAKALNVELDKIGDISLQHKKELT
                     ISSICTVFAESEVISLVSEGEELEDILYGIHKAIADRTMGLINRLGGVTPEVIMAGGV
                     AKNIGVVKALEKVLGTPIKIHVEPQIIGALGAAILSLEKVS"
     gene            4433..5785
                     /gene="rmuC"
                     /locus_tag="PK125_05265"
     CDS             4433..5785
                     /gene="rmuC"
                     /locus_tag="PK125_05265"
                     /inference="COORDINATES: protein motif:HMM:NF014680.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HPB37549.1"
                     /translation="METGLYIVGSAISGALIAWLIAFIRVQRRFAELENRAVSAETLA
                     KEREHLVSQKNIELASLREGLALEKGLKIEAATRLEESQKNLQEQVERINSIEKKLVE
                     TFKALSLDALTKNTDEFKKYADEFIQLAEEKLKFQALEGKKELEGKKDLIDRSVQAID
                     KTLSEMQKKVEDVAKGNIAVSTLVQQHGEITTRLKETTERLSHVLASSKKRGEWGERM
                     AEDIMRLIGMAEGVNYIRQKTLECSSGRPDYTFLLPNKLKVNMDVKFPLDNYVHYLNA
                     ESDHERKRYREELLRNAKAMIRQVTSRDYINPSDNTVDYVIVFIPNEQVYSFINEAEP
                     TIMDEALRQKVILCSPFTLYAVLSVIRQAIDNFNLEQTTSEILKLLAEFNKQWTSYKD
                     KFRMMGDRLDAAKKEYDTLLTTRTTMLERPLKKIKELSGQQEVSIEETPALSEQQSFE
                     "
     gene            6104..6259
                     /locus_tag="PK125_05270"
     CDS             6104..6259
                     /locus_tag="PK125_05270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB37550.1"
                     /translation="MTNIIATIGFALRRIREEYVTTPSARPYMNSLTQKGVIKEKIHV
                     SFPGIRD"
     gene            complement(6666..7436)
                     /locus_tag="PK125_05275"
     CDS             complement(6666..7436)
                     /locus_tag="PK125_05275"
                     /inference="COORDINATES: protein motif:HMM:NF014003.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent chlorinase/fluorinase"
                     /protein_id="HPB37551.1"
                     /translation="MKTITLLTDFGLKDAYVGIMKGTVLSIFPDVRFVDITHEVDPQD
                     VREGAFIVEEYYTYFEKGAIHVAVVDPTVGSDRKPIIVRRGGHFFIGPDNGLFTLLYD
                     DATRVYAIENSRYMGKEIGKTFHGRDIFAPAAAYLASGVQLTKFGSMVKDPVTLPGIY
                     PVIKKNVLKGEVIRFDRFGNAITNINQTVIDDFIKQKAFTIQFQGMSFRKIQKSYYEG
                     EIVPLISSSGYLEFGYFKGNLRQKLGLKKGDQISITIS"
     gene            complement(7441..8469)
                     /gene="mtnA"
                     /locus_tag="PK125_05280"
     CDS             complement(7441..8469)
                     /gene="mtnA"
                     /locus_tag="PK125_05280"
                     /EC_number="5.3.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF004326.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046523 -
                     S-methyl-5-thioribose-1-phosphate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5-thioribose-1-phosphate isomerase"
                     /protein_id="HPB37552.1"
                     /translation="MTDHIYWKDGVLYVLDQRELPFKKTYVRCKGLIDTAKAIENMTV
                     RGAPLIGIVAGYGVALGVKEAVETKGQCSMADLDRVCHRLQKTRPTAVNLAWAIERMK
                     ATYRSYANNSDIADVMTEEAISIHVEDIENNRMLSLYGAELIDDGDTILTHCNAGALA
                     TGGYGTALGVVRAAHESGKEIKVLATETRPYLQGARLTVWELSQEGIPVELIPDNHVG
                     LLSANKGFSKVIVGADRIALNGDTANKIGTYMISLCARQFHIPFYIAAPVSTFDRYIG
                     KGTHIPIEERDGKEVKYLQGKLLTVKNVKARYYSFDVTPARLITSFITEKGIIEPPFK
                     KYIKMLFL"
     gene            complement(8585..10036)
                     /gene="gatB"
                     /locus_tag="PK125_05285"
     CDS             complement(8585..10036)
                     /gene="gatB"
                     /locus_tag="PK125_05285"
                     /inference="COORDINATES: protein motif:HMM:NF004012.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HPB37553.1"
                     /translation="MLNHSDFEGVMGLEVHAHLLTNSKIFCGCSTKFGAEPNSHTCPT
                     CMGLPGALPVLNKKVVDFAIKLGLALHCKINKRSVFARKNYYYPDLPKGYQISQYEKP
                     ICEHGYLDIYSNGNKKRIRILRIHMEEDAGKLVHENAIETGSYSMVDYNRSSIPLLEI
                     VSEPDIGTPEEAVTYLKALRDVLVYLEICDGNMEEGSFRCDANVSVKKKDTTELGTRT
                     ELKNLNSFRFIERSLAYEINRQIDLIMSGGQVVQETRFFNVEEGVTYSMRGKEEAHDY
                     RYFPEPDLLPLVVDDHWIDEMRKGLPELPSEKLERFVTEHCLPRYDAEILVSDKELAW
                     YFEEAIRFFPEPKTVSNWIMTELLRELKDGNVSPKDAVLSPSLLAELLSLIKVGTISI
                     KIGKEIFPELYKSGISPKLLVEQKGLLQISDETVIIETIDAIIDRFPKEVSDYRHGKE
                     KLFGFFVGQAMKEMKGKANPKLLNVLLMRRLKG"
     gene            10506..11243
                     /locus_tag="PK125_05290"
     CDS             10506..11243
                     /locus_tag="PK125_05290"
                     /inference="COORDINATES: protein motif:HMM:NF024587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidylate kinase-like family protein"
                     /protein_id="HPB37554.1"
                     /translation="MWENISTEKTKSFIERHFGAKDKDASGITAKPSVTISRQEGAGG
                     LAVASDLANYLEKQTASHETWTVFSQHLVAKVLEDHGHHEGIGGFMKEGHKGSLTDAF
                     EEFLGLHPSTWTLVEQTNATIMRLAQIGNVILVGRGANIVTKELENVFHVHLVGTLEK
                     RIKRAQEVFNLDEKAATKYIKQKDKSRRRYIKDNFDQDMDNPLLYHVVINTDLVQYNE
                     AARMIGNEIIAHFKLDKIPKLQKVGTI"
     gene            complement(11403..11630)
                     /locus_tag="PK125_05295"
     CDS             complement(11403..11630)
                     /locus_tag="PK125_05295"
                     /inference="COORDINATES: protein motif:HMM:NF018961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dodecin family protein"
                     /protein_id="HPB37555.1"
                     /translation="MGVEGRAARNTVIIASSPKSFHDAVNVGFQRASKTLRGITGLRV
                     SDQRCRVEGGKIAEYRVTLEVIFLLEERRAL"
     gene            complement(11721..13535)
                     /gene="typA"
                     /locus_tag="PK125_05300"
     CDS             complement(11721..13535)
                     /gene="typA"
                     /locus_tag="PK125_05300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HPB37556.1"
                     /translation="MERGLIRNIAIIAHVDHGKTTLVDQLFRQSGMFRDNQVVEERLM
                     DSIDIERERGITIASKNGSFIYCNHYVNIVDTPGHADFGGQVERVLKMVDGALLLVDA
                     VEGPMPQTYFVLKKALALHLPIIVVVNKIDKPAARCDWVVNEVFDLFVKLDAPDHILD
                     FPIIYTSARDGYATHDHHVREGTMIPLFEMIIDSIPPPAGNPAGPFQMLVSSLSYSQF
                     LGRLAIGKITSGKLRIDQEVVISRAGSVPLPAGIKKIYKFRGAEMVETDEANMGDIVA
                     VAGMDNITIGETLTDPLNPIPLDGIEVDPPTVSMNFVPNDSPFYGKEGRFVTSRHLRE
                     RLFRETLSDVALIVEELGGVQGYKVSGRGELHLSILIEKMRREGYEFQVTRPQVIWKE
                     SKGKRLEPFEELTIDVDEKYMGRVIENLGGRRGILLEMYQDNGMARLHYKIPTRGLLG
                     FRSEFLTETRGMGIMNYAFLGFDEYAGEIKNRDKGVLIAMEECTTVAYALFNLQERGR
                     LFLGPGEKVYTGQIIGEHSRETDIIVNPAKGKKLTNMRAAGSDDAVVLTPHVQMSLEE
                     CLAYINDDELVEVTPQSIRLRKQILNGLERKRSKNVAA"
     gene            13786..15354
                     /locus_tag="PK125_05305"
     CDS             13786..15354
                     /locus_tag="PK125_05305"
                     /inference="COORDINATES: protein motif:HMM:TIGR02262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016405 - CoA-ligase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="benzoate-CoA ligase family protein"
                     /protein_id="HPB37557.1"
                     /translation="MIHGMTQEFFNAADYFVDRNVRQGRGHKVAVFTEHRNYTYNDIQ
                     KMTNKTANALRELGVTVDDRVLILMLDVPQFYAIFWGTIKIGAVPIPVNTMLTPDDYE
                     YYLNDSRAKVLVISDQMVPIISQIKGDLRYLRDLIVISETAGARIPFKQKYRHAPADI
                     KTEFTTKDDVGFWLYSSGSTGSPKGAIHSQYDMVVVSEGFGKGILGLTENDIIFSAAR
                     LFFAYGLGNSCCLPFGAGASVVLNSDAPKPENVIRHIMRFRPTVFFGIPTLYGQILEY
                     LEKQDGARPDPNSAHEFSSVRICFSAGEALPPELYHRWKKRFGIDILDGIGTTELLHV
                     FICNRPGDVKPGSTGKPVPGYELKLVDDDGHEVPKGQIGTLLVKGGSAAQQYWRKREK
                     TLLTMQGEWINTGDKYYEDEDGYFWCAGRGDDMLKVGGIWVSPVEVENCIMEHPAVRE
                     VAVVGAADEKNLIKPKAFVVVRDGHEPSPDLGKEIQQWTLDRIAKYKYPRWVEFVREL
                     PKSSTGKIQRFKLR"
     gene            15480..16487
                     /locus_tag="PK125_05310"
     CDS             15480..16487
                     /locus_tag="PK125_05310"
                     /inference="COORDINATES: protein motif:HMM:NF014045.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HPB37558.1"
                     /translation="MSNVAILWDMENVNPGSDSLFLEGLTDYAEAKGRVVAARAYGNW
                     NHTSLSKLAPSLTRRYFYLVHIPKGQKNSSDMVLVSDALEILRVSDHIDTFILVTGDS
                     DFRFLVLSLRRAGKIVHIVCNTKNASEDLLALADSYVDYRELMPGGSDEESGVKPAFS
                     SGDKAPPEKIPAPTMSQEDWFQILAEAADLMLKKKTQPGIGSLKIRMKMLNPNFDEKR
                     LHFSHWSEFVTAAVKAGFVTIEGKGSEALVYPVTSVLQKAGTQQKAFLTLLEVLKELD
                     KGNKSIYHSYRSVNSKLIEKKIYFNDLGFNQFKEFIQAAESRGLVESKVEGLKHSVKR
                     L"
     gene            complement(16538..16972)
                     /gene="ssb"
                     /locus_tag="PK125_05315"
     CDS             complement(16538..16972)
                     /gene="ssb"
                     /locus_tag="PK125_05315"
                     /inference="COORDINATES: protein motif:HMM:TIGR00621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HPB37559.1"
                     /translation="MSSLNKAIIIGNLGADPEVRYTASGMAVATFNVATKETWKDKNN
                     SKQDRTEWHRVVAWGKLGEQAGEYLKKGRLVYVEGRMQTREYEGKDGIKRKTFEIVAS
                     MFRMLGPSGQQGDREGYKTQGSLQPSGDDDFIPEIEEDDVPL"
     gene            17246..18859
                     /locus_tag="PK125_05320"
     CDS             17246..18859
                     /locus_tag="PK125_05320"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HPB37560.1"
                     /translation="MDDRGKTKEQLINELVEMRQQVAVLEQTRAAKNHVEQTLKNTEE
                     ILRLILNLSTNFSILSSNEIHEGIDDVMKIVGEFVGVDRSCMFEFSDDGRTVQNTHEW
                     CGLGIDPLIKNRQSIPVSKLPSFIQPIWDLEVVNISDLPKITRKNKGKNKLAQSIIAI
                     PLVYNISLMGFLGFESIHKKKVWSESTVSLLKIVGETFTSALMRKRTEDSLRESESHF
                     RNIFDYAVEGIFQSTLDDRYKSVNPAFARTLGYESPEEVLNTITNISRQLYVNPESYQ
                     TLKALLSERGFVEGYETEFYKKDGSKIWVAINARAVRNGNGSIDFFEGFHQDITRRKR
                     AENDLKNTVESLKKTLDGTVHALSTTLEMRDPYTAGHQKRVALIACEIAEEMGLPEEQ
                     IEGIRVMGFLHDIGKIMVPAEILSKPGKINEYEFHIIKAHSQVGYDILKGIELPWPVA
                     LATIQHHERIDGSGYPQGLKGDEIILEAKILAVADVLEAMASHRPYRPALGLDKALEE
                     IREKKGVYYDPVVVDACLRLFVDKGFRYE"
     gene            complement(18900..19205)
                     /locus_tag="PK125_05325"
     CDS             complement(18900..19205)
                     /locus_tag="PK125_05325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB37561.1"
                     /translation="MPAWYMAIMMETGKVQWRPKVNADVSDTGPDDRKLIIEFEGDLE
                     NMPWVSNLSCGSAKVDLNILAATVPELFDKGWLKDRGPQEASIAIMGDHNIIEITVE"
     assembly_gap    19443..19542
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            20144..21130
                     /locus_tag="PK125_05330"
                     /pseudo
     CDS             20144..21130
                     /locus_tag="PK125_05330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012222646.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS481 family transposase"
BASE COUNT         6093 a         4720 c         4603 g         5742 t
ORIGIN      
        1 tcagctcaaa aggacccata ggatagttga gaccaagctt ggctgcctta tcaatgtctt
       61 cttttgtggc tacaccctgt tccagaagtt tcatagcttc aatgaaatgg ggtatcatga
      121 gcctgttaac gataaaagcc ggcacgtcct ttttcacttc aactggctct tttcctaatt
      181 ttttcgttag atctatggtt attgcaaccg tttcgtcact ggtattgtat ccccggacaa
      241 cctcaactaa tctcatcaga ggcactggat taaaaaaatg catgcccaca accttgtcag
      301 gcctcttggt tgcagttgca atctcagtga tggacatgga agaagtattg gatgaaagaa
      361 ttacatcttt cctcgttagt tcatcgagct ctgcaaaaac cttcttcttt acatccataa
      421 cctcgatgat aacttcgact acaaagtcga catctttgag gtcagccatt gcagttgtac
      481 ctttaattct acccataacc tctttcttgg catcagcagt cattttccct ttttcaactg
      541 actttgtaag aaatttatca atgtttttga ttccgctttc tacaaatcta tcttcgatat
      601 ctctcatgat aacattgtat ccagccatgg aagccacttg agcaatacca ttgcccatta
      661 cccctgcccc taaaacccca attgtcttta ttgccataaa aaacacctcc tattttttta
      721 atgggtacta tcagatcaaa taaaaaaatt cacatggtca tatatctaac tattattgga
      781 ttatgtctgt caactatttt ctatgataag ccgtgtttat tattaagaat attgttttca
      841 ataccagact tctatcaaaa cgacccgggg catttatacc atatttaatt ctgattctac
      901 caatagaggc tcggggattt attgtgaaat ttccttcttt cactattttg agagttctaa
      961 ttttgaagct tccttgccag gtgggtaacc cgggctgtta aatttagtaa atatgacttc
     1021 tctgactgct taacacccct gtttgtgtca tactgtcgtg cttttatacg gtgaacaacc
     1081 ctttcaatag aacctccttc agatatcagg agatcttgca catcatcccg gaattttttc
     1141 gcagcctcta aagaacggtc aaaaaatctt ttcacatcat cacctacaaa gacgaaatgg
     1201 tgaccctggc acaaaacatc aatgtcgagt ttcttcagtt ccttaaggga tgcaatatac
     1261 aaatcatagt ccacaagaaa ttcagggatt atctggtctg cctgatcgag gactcccgca
     1321 gattctgtag cgaaaagaac tcgcttttcg gggatataat agcttaacat atcccgcgta
     1381 tgcccgggcg ttgcaatcac ctgcaatgtc agaccacctc cgatatttag agtctggccg
     1441 ccatccacga taatatcgat ttcaaaaggc tcaaaggaat cccgtattag tttttctttg
     1501 tcaacaccat gtacctgacc aacaaggggt atcacatcct gactcagcct cttcataaga
     1561 ttctgtgcat ttgggcgccg tataatctca gcagatatcc gtgaggctgc aaccttaagg
     1621 ccggggaaac acttcttcag gtatgctgtt gacccacaat ggtcccaatg gacgtgggta
     1681 aggaacagtg tttttgggtt cctgccattc agaaagtctc tgatgtcttc ttcatagagg
     1741 cgtccggcac agtgaaaacc cgcctcaaac aaaaccggtt cttcagctgc aacaacatac
     1801 acaggagacc atgcaagccc tgcaacgaaa aagatttctt taacctgtcc tgttttattg
     1861 aaaatcagaa aaacctcctg aaaagtctgg attatgataa ttatataata tatagttacc
     1921 ttatgtgctt caagtttttt gtggttgtac aggtgggtga tcgcaagtaa tttttgtaat
     1981 tgtgaaaaaa gtagttgaca tgccaaaatc gctttgttaa gataccttgt taatagtttc
     2041 acattcttga ggaaaggaga gggatattat ggatttcaag atttccgatg aattagaatc
     2101 aatgcgaaaa atggcctatg attttgcagt aaagaatatt aagcccacca tggaagaaga
     2161 tgagaaggag cacaagtttc gcccagagat tatgaagaag atgggtgatc agggaatgtt
     2221 tgcctgtata gctccggaga agttcggtgg cctcgcactt ccagaaggtc atatggctgc
     2281 caccctgatg gctatggaag tagcaagaat tagcccttcc tggggccttc cgttcaacct
     2341 tcagatgaac ggtccgcaga acgctcttat gaaattcggt tcagaagaat tacaggagca
     2401 gtatttaccg ggccttatcg ctggaacaag cggtggttgc tttgccatca ccgagccaaa
     2461 ttcaggctct gatgtagcaa gcatgaagac aacagcgaca gaggttgacg atggctttat
     2521 cctgaacggc acaaaaacat ggatttccgg cgtgcccttc tgtgattgcg gtattgttta
     2581 cgctatgacc gacaaggctg ctaagcataa aggtatgtct gcctttttcg ttgatttcaa
     2641 aacccctggt atcgtacaga gagctatcac cacaaaactt ggactttttt gtgcacccac
     2701 aggcgaaatc ttcttcgaag aagcaaagat acccaagagc gccctcgttg gcaaactcgg
     2761 ccagggattt gccatttgca tgacgatgct caacttcact cggctcagtt ctgctgcacg
     2821 ggcagtcggt gtggcacaat cctgcattga agaagcgtgt aagtacgcaa atgagagaga
     2881 acagttcggt cagccaatag gacagttcca gatggttcag gaacagattg gaagaatgtc
     2941 tgttgaacat gaagcagcaa aacttcttct gctcagggct gcatggcaga aagatcaggg
     3001 cgcaaacaac actttagaaa cttcaatggc aaaatattat tgtgcagagt ctgcaaactt
     3061 ctgcgcttca gaagcagtca aaattttcgg atcatacgga ttctcatcag aatatcctgt
     3121 tgaaaggtac cttagagatg caaaatcata tcagatagtt gaaggtactt ctaacgttca
     3181 gaagatgata attgcccagt tcgctctggg ttacagaaag gcgtaaacag taaatctcaa
     3241 ggaggtaaat cggtatggca acagaagtta cagttccaat ggttggaaaa attgtgggcg
     3301 tgaatgtgaa cgtaggcgat aaagtcaatg aggacgacca gattgccacg ctggaagcga
     3361 tgaagatgga aatgcccatt gtttccccgg tatccggcac agtaaaggaa attaaagttg
     3421 ctgccggaca ggaagttgaa gcagatgcag ttctagcaat tattgaataa tacaataacg
     3481 ggaaggtgat tccatgctag ttgctggtat tgatattggc tctatcacaa cagaagccct
     3541 tctttttgat aaggacaaag ggattgtcgg atatacaatc ctgcagacag gcgctgattc
     3601 aaaaaagact gctgaaattg cacttgaaaa ggtcctatct tatcctggca aaagcatatc
     3661 tgatgtctcc tatgtcattt caactgggtg cggaagaaag agagctgcct ttgccaagga
     3721 agccgtcaca gaaataacgt gcattgcaaa gggggtaaac tatctcttcc ccgaggcacg
     3781 gactatcata gacattggcg gacaggacac aaaggttgtc cggatagacg agaaaggacg
     3841 cgtcttggaa tttgaaatga acgacaagtg tgcagctggc accggtcgtt tcatagaagt
     3901 catggcaaaa gcgctcaatg tagaacttga taagatcggt gatatctctt tgcagcacaa
     3961 aaaagagttg acgatcagca gcatctgcac tgtgtttgca gagtcagagg tgatctctct
     4021 tgtgagcgag ggggaggagc ttgaagacat tctgtacggg attcataagg caattgctga
     4081 cagaactatg ggtcttatca acaggttggg aggagtaaca cctgaagtaa taatggctgg
     4141 tggtgttgca aaaaatatcg gagtagttaa ggctttagaa aaggtcctcg gcactcctat
     4201 aaaaatccat gttgaacctc agatcattgg tgccttgggt gctgcaattc tatcactgga
     4261 aaaagtatcc tgatagaaaa aagtaaggac gtcaaaaagg actggcgatg attttgccag
     4321 tccttttttt gtatcatgac acactatatg caatgtcaca acatatggtt ggctcattcg
     4381 tagaattgca agaatcaatg attcgggaaa cgtcggagac agggaggatt tgatggaaac
     4441 tgggctgtat attgttggct cagcaatatc aggcgccctt atagcatggc ttatcgcgtt
     4501 cataagggtg cagagaagat tcgcagaact ggagaacagg gcagtatcgg cagaaacatt
     4561 ggcaaaagaa agggagcatc tggtttcaca gaaaaatatt gagcttgcaa gccttagaga
     4621 agggcttgct ctagaaaagg gtcttaagat agaggcagcg accagactcg aagaatccca
     4681 gaagaatctt caagaacagg ttgagcggat taattccata gaaaaaaaat tggtcgaaac
     4741 ttttaaagcc ctctctttgg atgcgctcac aaaaaatacc gacgagttca agaaatatgc
     4801 tgatgaattt atacaactgg cagaagagaa actcaagttt caggcccttg agggtaagaa
     4861 ggagttggaa gggaaaaagg atctgatcga caggagtgtt caggcaatcg acaagactct
     4921 gtcagagatg cagaagaaag ttgaagatgt ggccaaaggc aatattgctg tttccactct
     4981 ggttcaacag catggagaaa tcacgacgag gctgaaggag acgactgaac gtctgagcca
     5041 tgttttggca agctcgaaaa agagaggcga gtggggcgaa cggatggcag aagatattat
     5101 gcgtcttata ggcatggcag agggtgtaaa ctacatcagg cagaaaaccc tggaatgttc
     5161 atctggcaga cctgactaca cttttctcct acccaacaaa cttaaggtga atatggatgt
     5221 gaaatttccc ctcgacaatt acgtacacta cctgaatgca gaatcagacc atgagagaaa
     5281 gcgataccgc gaggagctcc tcagaaacgc taaggcaatg atccgccagg ttacgagccg
     5341 tgactatatt aatccttcag acaacactgt cgactatgtt attgttttta tccccaatga
     5401 acaagtttac agtttcatta atgaggcaga gccaacaatc atggacgagg ccctgcggca
     5461 aaaggtcata ctttgctcac cctttaccct ctatgcggtt ctttcagtaa taaggcaggc
     5521 aattgacaat tttaatcttg agcagacaac atctgaaatc ctcaaacttc tcgcagagtt
     5581 taacaagcag tggacatctt acaaagataa attcaggatg atgggtgatc ggctcgatgc
     5641 tgcaaaaaaa gagtacgata cccttttgac tacaagaaca accatgctcg aacgacccct
     5701 gaagaagatt aaagaactga gcggccaaca agaggtaagc attgaggaaa cccctgccct
     5761 ctctgaacaa cagtcctttg aatagttagc ctttgtattc caatccctgc attcaattga
     5821 aagactttga actattatcc agctcaatta cgatgtaaat gatggctcat actggggtat
     5881 tcaatatata tatatcagtg ctgaatattt gaggaaaagg aagtagcagg tatcaaaatt
     5941 acgtcgggca tatggaacga actatatccg ccatcccttt tcttcctcaa agaaggaact
     6001 atcatttcaa aacttgttta tggagaaccg atcgaccttc tttttgttaa tggccatggc
     6061 tatatgtcat ccctattctt acggtctggt aaaagtggtg ggcatgacca acatcatcgc
     6121 gacaatcggg tttgcccttc gacgtattag agaggaatac gttacgaccc catctgcccg
     6181 cccgtacatg aactccctca cccaaaaagg cgttattaaa gaaaaaatcc atgtatcttt
     6241 cccagggatt cgggattagc ctgaaaagcg cgtagaagaa tatcgcaagt ggaccgctca
     6301 gagaaaaaat accggaacca ttgtgccttg ccagctgtat tcaaaaaatt ggctacggag
     6361 aggagaatca attcatgaat gacgaagaga ggttgaagat agtcattgga cagatgttgg
     6421 agtacaacag aatacttgtt cgagattaag aaaaatggac acttttcgca aaagctagtt
     6481 aattggttga tacaggttct tatcttgttg aagcaaaaaa tcttaatccc aatgtaggtg
     6541 ctgccttaaa aacagggtct gtgccattta tctcttagtg aagctgcggt tgatggagaa
     6601 aaagatttat gaccttcgca ctaaagaaat cgagcaaata atgagcgcgc atctaaacct
     6661 gtattttacg agattgtgat agatatctgg tcaccttttt tcaggccaag tttttgcctg
     6721 aggttacctt tgaaataccc aaattcgaga taacctgagc tgctgatgag aggaacaatc
     6781 tcaccctcat agtaactttt ctggattttt ctaaatgaca tgccttgaaa ctgaatagtg
     6841 aaggcttttt gctttataaa atcatcaatc acagtttggt taatatttgt gattgcattg
     6901 ccaaaacgat cgaaacggat gacttcaccc ttcagaacat tttttttgat gacagggtaa
     6961 atgcctggga gtgtgaccgg gtctttaacc atgctcccaa acttggtaag ctgaaccccg
     7021 gaagccagat aggctgctgc cggggcaaag atatcccgcc catggaaggt tttgcctatt
     7081 tctttgccca tgtatcggct gttttctatg gcataaaccc gggtagcatc atcatatagt
     7141 aaagtaaaga gaccattgtc agggccaata aaaaagtgtc cacccctcct tactatgatc
     7201 ggttttcggt cacttccaac cgttgggtca acgactgcca catggatagc ccctttttca
     7261 aaatacgtgt aatactcctc gacgataaag gctccttccc gaacatcctg aggatcgacc
     7321 tcatgggtaa tgtccacaaa tcttacatca ggaaaaatag agaggactgt acctttcatg
     7381 ataccgacgt aggcgtcctt taagccgaaa tctgtaagta aggtaattgt tttcacggct
     7441 tcacaagaaa agcattttta tgtatttttt aaagggcggc tcgataatcc ccttctcagt
     7501 tataaagctg gtgatgagtc tggcaggagt gacatcgaag gaataatagc gcgcttttac
     7561 attttttacg gtcagaagtt taccctgtag gtacttgacc tctttcccat ctctttcttc
     7621 tattggaatg tgggtgccct ttcctatata cctgtcgaat gttgaaacgg gagctgcaat
     7681 atagaagggt atatggaatt gacgggcaca gagggagatc atatatgttc ctatcttgtt
     7741 tgctgtgtct ccgttaagtg ctatcctgtc tgcgcctact ataactttac tgaaaccctt
     7801 gttggcactc aggagtccca cgtgattatc tgggataagt tcaacaggga ttccttcctg
     7861 agagagttcc cacacagtta gacgtgcccc ctgcaaatag ggtctcgtct ctgttgcgag
     7921 aaccttgatc tcttttcccg attcatgggc agcacgcact acgccaagtg cagtaccgta
     7981 acctcctgtt gcaagagcgc ctgcattaca gtgggtaagg atggtgtccc catcgtcgat
     8041 gagttccgcc ccgtagagag aaagcatacg gttgttttca atgtcttcca catgaatgga
     8101 gatggcttct tccgtcatga catctgctat gtcagaattg ttcgcatacg acctataggt
     8161 ggctttcatt ctttcaatgg cccaggcgag atttacagct gttggtcttg tcttttgcag
     8221 cctgtggcag acgcggtcta agtcagccat actgcattgg ccttttgttt caactgcctc
     8281 cttcactccc agggcaactc catatcctgc aacaatcccg ataagaggag caccacgcac
     8341 agtcatgttc tcgatagcct tcgctgtatc aatcaaacct ttgcatcgaa catatgtctt
     8401 tttaaacggc agctctcgct ggtcaagaac atagagcaca ccatctttcc aataaatgtg
     8461 atcagtcaag tctacccctt atttcgtatt cgcatattgc agggccgtat cttttatttc
     8521 ccataaagag gctgaaatgt cgtgttgatc ctgccatcaa aatacggcac actgtatacg
     8581 gcttctaccc tttcagtctc ctcatgagaa gtacattgag aagcttaggg tttgctttgc
     8641 ctttcatctc cttcatggcc tggccaacaa aaaacccgaa tagtttttct tttccatgcc
     8701 ggtaatcgga tacctctttg gggaaacgat caataatcgc atcgatcgtt tcgataatga
     8761 ctgtttcatc tgatatttga agaagccctt tttgttcaac aagtagtttc ggtgaaattc
     8821 cagacttgta caattcaggg aatatttctt tacctatttt gatacttatg gtaccgacct
     8881 tgatgaggct aaggagttct gcaagcagtg aaggtgacag tactgcatct ttgggagaca
     8941 cattgccatc tttcagctct cttaaaagtt ctgtcatgat ccagttgctc accgtttttg
     9001 gttcaggaaa gaaccgaatc gcttcctcaa agtaccatgc gagctccttg tctgacacaa
     9061 gaatctccgc atcgtatctg ggtaaacaat gctctgtgac gaatctttct agtttttccg
     9121 aaggcagttc aggtaaacct tttcgcatct catctatcca atggtcatcc actacaagag
     9181 gtaaaaggtc tggttcaggg aagtatcgat aatcatgagc ttcttctttc cctctcatgg
     9241 agtaagtcac accctcttcc acgttgaaga aacgggtctc ctgaactacc tgccccccgc
     9301 tcatgatgag gtcaatctgc cggttaattt cataggccaa cgacctttcg ataaaccgga
     9361 aagagttgag gttcttcagt tctgttctag ttcctaattc tgttgtgtct ttctttttta
     9421 ccgagacatt ggcatcacaa cggaaactgc cttcttccat gttgccatca cagatttcaa
     9481 gataaacgag tacatcgcgg agagctttca ggtatgttac cgcttcctcc ggtgtaccga
     9541 tgtctggttc gctcacgatt tcaaggagag ggatgcttga tctgttatag tcaaccatac
     9601 tgtaactgcc tgtctcgatg gcattctcat gcacaagctt gcctgcatcc tcttccatat
     9661 gaatccttaa aatccgaatc cgcttcttgt tgccattact gtaaatatcc agatagccat
     9721 gctcacatat gggtttttca tattgtgata tctgataacc tttcgggagg tcaggataat
     9781 agtagttttt acgtgcaaag acacttcttt tatttatttt gcagtgaagg gcaagaccga
     9841 gttttatggc aaaatcaacc accttcttat tgagaacagg cagagcccct gggagcccca
     9901 tgcatgtggg gcaagtatgg ctgttcggct ctgcaccgaa tttggttgaa caaccacaga
     9961 atatcttgct gtttgtgagt aggtgggcat gaacttcaag gcccatgaca ccttcaaaat
    10021 cactgtgatt caacattaac tccttttgaa atcttgtttt ctatccatct gtaatttttg
    10081 aaaatataca ccagatagcc ggtcttttca aagaattcca tacattcttt tcaacgctta
    10141 cctgtttatc agtaaaacgt ttgtgaacct gtcccatggt tcccccttct ttttgaaaca
    10201 ggttactatt taggtgtgca cttttaattc ttaaatgaca gatttgagaa acgtgttgac
    10261 aaaaactaat ttacctatat actgaataat gtagatgaaa tctgcgatat taccggggtg
    10321 aatcgattag cgggttttgg aacttcacaa tcaggaggtg atgtcaaaga cgcataaaac
    10381 aggccgccta ggttagtgca ataagagttt cgtgtatgga tgaccggcaa cataccattg
    10441 cccactctgt cattctcaaa aagtgggaca acaatttttg tactgcagac atagggggat
    10501 aaaatatgtg ggagaatata agtaccgaaa aaactaagtc ttttatcgag cgccactttg
    10561 gtgcaaaaga caaggatgcc tccggaatca cggcaaaacc gtcagtaact atttcacgac
    10621 aagaaggagc tggtggactg gcggtggcct cagacctagc aaattatctg gaaaaacaga
    10681 ctgcatcaca cgagacatgg acagtctttt ctcaacatct cgttgcaaaa gtacttgagg
    10741 atcacggcca tcatgaaggt attggcggtt tcatgaaaga aggccataag ggctccctga
    10801 ctgatgcgtt cgaagagttt ctgggcttac acccaagtac ctggaccctc gtggaacaga
    10861 caaatgcaac aattatgcgt cttgcacaga ttggaaatgt tatcctggtt ggccgaggag
    10921 ccaatattgt caccaaggaa cttgagaatg tttttcatgt gcatcttgtg ggaacccttg
    10981 agaagaggat aaaacgggcc caggaggtct tcaatcttga tgaaaaagca gctaccaagt
    11041 acataaaaca aaaggataag tcacgtaggc gatacataaa ggataacttc gaccaagaca
    11101 tggataatcc gttgctatat catgtggtta ttaacacaga cctggttcaa tataacgaag
    11161 ccgcccggat gattggcaat gaaattatag cacattttaa gctggacaaa ataccgaagc
    11221 tacaaaaggt tggaaccatt taaaaaaaag aaattacctt gttaggaaac acggatacct
    11281 gcaatattct ataggattac agtgcaggta cggggatacc gcaacgagat tagtttcaat
    11341 atttttcccg aacctcaatt ctctgtgagg atgatttgct cgatatttgt tttgtccccc
    11401 gcctaaagtg cgcgcctctc ctcgagaaga aaaataacct ccagggttac tctgtattca
    11461 gcaatttttc caccttctac tctacaacgt tgatcactta ctcgcaatcc agtgatacca
    11521 cgcaacgttt tggatgctct ttgaaaacca acattaacgg catcatgaaa acttttcggg
    11581 gaacttgcta tgatcaccgt gtttctcgcc gcccgtccct caacacccat atgaaccctc
    11641 cgttttttta caaaaggtaa caggaagagc attactgagt caaatcattt tctatctttt
    11701 ctgtggaata cagggtttcg ttacgctgct acattctttg accgtttacg ttcaaggcca
    11761 ttcagtattt gctttctgag ccttatagat tgaggagtaa cctcaacaag ttcatcgtcg
    11821 ttgatgtacg caaggcactc ttccaggctc atctgcacat gaggggttaa aacaactgcg
    11881 tcgtctgaac ctgctgcacg catattggtc agctttttgc cctttgcagg attcactatg
    11941 atgtctgtct cacgggaatg ctcccctata atctgaccgg tatagacttt ttcaccaggt
    12001 ccaaggaaga gcctccctct ttcctgaagg ttaaagagtg cgtaggctac tgtggtacac
    12061 tcttccatgg caataagaac ccctttgtct ctgtttttaa tctcaccggc atattcgtca
    12121 aaacctagga atgcatagtt cataataccc atacctcgtg tttcagtaag aaactccgag
    12181 cggaatccga gcagccctct cgtgggaatt ttatagtgta gccgtgccat gccgttatcc
    12241 tggtacattt caaggagtat acctcttctc ccacccaagt tttcaatcac tcttcccata
    12301 tatttttcat caacatcaat ggtcaattcc tcaaaaggtt caagtctctt ccctttagat
    12361 tctttccaga taacctgagg tcgtgttacc tgaaattcgt atccttccct tctcattttc
    12421 tctatcagga tggacaggtg aagttcgcct cgcccggaaa ctttataccc ttgaacacca
    12481 ccaagctctt caacgattag ggctacgtct gacagtgtct cgcggaaaag cctttcccgt
    12541 aaatgacggg atgtgacaaa ccggccttct tttccataaa acggtgagtc attaggaaca
    12601 aaattcatgg aaacagttgg tgggtccacc tcaataccat cgaggggaat aggattcaat
    12661 ggatcggtta gcgtctcgcc aatagttata ttgtccatgc cggcaacagc cacaatatcg
    12721 cccatgttag cttcatccgt ttctaccatt tcagcgccac gaaatttata gatcttcttg
    12781 atgcctgcag gtaaagggac tgatccagct cttgagataa ccacttcctg atctatcctt
    12841 agctttccag aggtaatctt tccaattgcc agtctaccaa gaaactgtga ataactgaga
    12901 gaactaacaa gcatctgaaa gggccctgca gggtttcctg caggcggggg aatggaatca
    12961 ataatcattt cgaataaggg tatcatcgtc ccctctctta catgatgatc gtgggttgca
    13021 taaccatccc ttgctgatgt gtaaatgata gggaagtcaa ggatgtggtc aggtgcatca
    13081 agcttgacaa aaagatcgaa gacctcattc accacccagt cacatctagc cgcaggctta
    13141 tcaatcttat ttaccaccac aatgatgggc aggtgcagag caagggcttt ctttaaaaca
    13201 aaatatgtct gtggcatagg cccttccacc gcatcaacaa ggagaagggc accatcaacc
    13261 atcttaagaa cacgttcaac ctgccctcca aagtctgcat gtccaggcgt atcgacaatg
    13321 tttacataat gattacagta gatgaacgac ccgttctttg atgctatagt gataccccgt
    13381 tcccgctcaa tatcaatgga atccatgagt ctctcttcaa ctacctggtt atccctgaac
    13441 ataccactct gcctgaatag ctggtctacc agggtggtct tcccatgatc cacgtgtgca
    13501 ataatagcga tattccttat caaaccccgt tccattcttc ctcaatattg tatatagaat
    13561 gttgtaagaa gatttatcag aagcgtaata ctataacaga caatatgcct caaggcaata
    13621 taaatgcata agaatctggt tgaaaaccat atcgttttta tggttaaata attttgtcag
    13681 ttgtcatttt atctgcattc tttgcaggtt ttcttttgaa acaatggtgt ttcacatttc
    13741 ttagctgtaa atactgtgca aacattctcg aaaaggaggt tttttatgat tcacggaatg
    13801 actcaggaat ttttcaatgc tgctgattat tttgttgaca ggaacgtgcg ccagggtcgg
    13861 ggccacaagg tggcagtgtt tacagaacat cgcaattata cctacaacga catccagaaa
    13921 atgaccaaca aaactgcaaa tgcgctccgt gagcttggcg tcaccgtcga tgaccgtgtt
    13981 ctcattttaa tgcttgacgt cccccaattc tacgcaatat tttggggaac gataaaaatt
    14041 ggggctgtgc ccatcccggt caacactatg ttaacccctg atgattatga atattacttg
    14101 aatgacagcc gcgcaaaggt actcgtcata tcagaccaga tggtaccaat tatatcgcaa
    14161 atcaagggag acctgcgcta ccttcgcgac cttattgtca tatctgagac ggcaggtgcg
    14221 cgtatccctt ttaagcaaaa gtacagacac gcaccggcgg atatcaaaac agaatttacc
    14281 acaaaggatg acgttggttt ctggctttac agttcgggct ctacagggtc accaaaggga
    14341 gccattcatt cgcagtatga catggttgta gtcagtgaag gatttggcaa gggcatactc
    14401 ggcctcactg agaatgacat aatcttctcc gcagccagac ttttctttgc atacggcctt
    14461 ggcaactctt gctgtttgcc ttttggagcc ggtgcctcgg tagtccttaa ttctgatgct
    14521 cctaagcccg aaaatgtcat ccgtcacatc atgcgctttc gccctacagt ctttttcggt
    14581 attccaacgc tttacggtca gattcttgaa tatttggaaa aacaggatgg agccagacct
    14641 gatccaaact cagcccatga attttcatca gtccgcatct gcttttcagc aggagaagca
    14701 ctgccacctg aactttacca tcgttggaag aagcgttttg gcattgatat ccttgacggt
    14761 atcggaacta ctgaacttct ccatgtattc atttgcaacc gtcctggtga tgtgaaacct
    14821 ggctccacag gaaaacctgt acccggttat gaattaaaat tggttgatga tgacgggcac
    14881 gaggtgccaa aaggccagat cggtacgctt cttgtaaagg gtgggagcgc tgcccaacag
    14941 tattggcgga aacgcgagaa aacactcttg acaatgcaag gtgaatggat aaatacgggc
    15001 gataaatatt atgaagatga agatggctac ttctggtgtg ctggccgtgg tgatgatatg
    15061 ttgaaggtcg gcggcatctg ggtttcgccg gttgaggtag aaaactgtat catggaacat
    15121 cccgctgtca gggaggttgc tgttgtcggc gcagcagatg aaaagaacct tataaaacca
    15181 aaagctttcg tcgttgtgag ggacggacat gaaccatctc cagatcttgg aaaggaaatc
    15241 cagcaatgga cacttgatag aattgcgaaa tataaatatc cacgatgggt agagtttgta
    15301 agggaattgc caaagagctc caccggcaaa atccagagat ttaagctgag gtaaaaaagc
    15361 agactcatta accagacaac gataggctcc tgttttgttg ctgctatttc acaaaataaa
    15421 ttctatacta catagatgtc tgtgggaaat aggattgttt ttgcgaaagg ggagacaaaa
    15481 tgagtaacgt ggctattctc tgggatatgg aaaatgtgaa tcctggatct gattccttat
    15541 ttcttgaagg gcttaccgat tacgcggagg ccaagggaag ggttgtagct gctcgagctt
    15601 acggaaactg gaatcacaca agcctttcca aacttgcccc ttccttaacg agaaggtatt
    15661 tttatcttgt tcacatccca aaagggcaga aaaacagttc tgacatggtt ctggtatccg
    15721 acgccttgga aattttgagg gtttccgacc acatcgatac ctttatcctt gttacagggg
    15781 acagtgattt tcgcttcctc gtcctttctc tgagacgcgc cggaaagatc gtacatatcg
    15841 tttgtaacac caaaaatgca tcagaagatc tcctcgctct cgccgatagt tatgtcgatt
    15901 accgcgagct catgccaggt ggaagcgatg aagaatcagg cgtcaagcct gctttttcat
    15961 caggagataa ggctcctcca gagaaaatac cagcccccac catgagtcag gaggattggt
    16021 ttcaaatcct cgctgaagct gccgacctca tgttgaaaaa gaaaacacag cctggcatcg
    16081 gttccctgaa gattaggatg aaaatgctta atcctaattt tgacgagaag agactacact
    16141 tttctcactg gagtgagttt gtgactgcag cagtaaaagc aggctttgta accatagaag
    16201 gcaaagggtc cgaggcccta gtatacccag ttactagtgt tttacaaaag gccgggacac
    16261 agcagaaggc ttttttaacc ttgctggagg tattgaagga actggacaag gggaataagt
    16321 ccatatatca ctcttaccga agtgttaact cgaaacttat tgaaaaaaag atatatttca
    16381 atgatctcgg tttcaaccag ttcaaagagt tcattcaagc tgctgaatca cgaggccttg
    16441 tggagtcaaa ggttgaaggt ttaaagcatt cggtgaaacg actttaataa ttcaacagtt
    16501 taaacggttt caacagttca agccgtttaa acgttcatta aagcggtacg tcgtcctctt
    16561 caatctcagg tatgaaatca tcatctccgc ttggctgaag gcttccctgg gttttatatc
    16621 cttccctgtc accctgttgt ccggaagggc caagcattct gaacattgaa gccactattt
    16681 caaaggtctt ccgtttaatg ccgtctttac cctcgtattc acgggtctgc attcttccct
    16741 caacgtaaac aagccttcct ttcttcaaat actccccagc ctgctcaccg agtttccccc
    16801 atgcaacaac cctgtgccat tcagtcctat cctgtttcga attattttta tccttccatg
    16861 tttcctttgt tgcgacatta aatgtagcta ccgccatacc gctcgcagta tagcgtactt
    16921 cagggtctgc accaagattt ccaataataa ttgccttatt caaactcgac atttctaacc
    16981 cccatgagca agagtttcaa ctgtttaaac tgtctgaatc atttaaacaa gctgttatga
    17041 gcacaaatgt tatagcatta actggtaaat gtcaacgaga tgaaaaaagc tgttatcaca
    17101 aaatgataat gtcctctatt gccaaaacag aaatgtgaaa ataatgggcg ttaaagttac
    17161 gagacctcat gccgataagt acgtaaggct attggtataa atgctgctac tttatcataa
    17221 ataacgatgg atagatctct tcgacatgga cgatagaggc aaaacaaagg agcaactcat
    17281 caacgagttg gtcgaaatgc ggcagcaggt agccgtgtta gagcaaacaa gagccgcaaa
    17341 aaatcatgtg gaacagaccc tgaaaaacac cgaagagatc ctgagactta tacttaatct
    17401 ttctacaaat ttcagtatac tttcttcaaa cgagattcat gagggcattg atgatgtaat
    17461 gaaaatagta ggggaatttg ttggtgttga tcggtcatgc atgtttgagt tctctgacga
    17521 tggccgcacc gttcaaaata ctcatgaatg gtgcggcctt ggtattgatc cgcttataaa
    17581 aaatcgccag tccatacctg tttctaaact tccttctttc atccagccaa tctgggacct
    17641 tgaagtggta aacatctctg atcttccaaa aatcaccagg aaaaataagg gtaaaaacaa
    17701 attagcccaa tcaattattg caattcctct cgtctacaac atctcgctca tggggtttct
    17761 aggctttgaa agtatccaca aaaaaaaggt gtggtcagaa tctacagtct ctctgttaaa
    17821 aatcgtagga gagacattca caagtgctct catgagaaaa cggaccgaag actcgctcag
    17881 ggagtcagaa agccattttc ggaatatttt cgactatgct gttgaaggaa tctttcaaag
    17941 cactcttgat gatagataca aaagtgtcaa tcctgctttt gcccgcacct taggatatga
    18001 gtcaccggaa gaggttctca atacaattac caatatatct cgtcaactct atgtcaaccc
    18061 tgaaagttat cagaccctta aggctctttt gtcagaaaga ggtttcgttg agggatacga
    18121 gacagaattt tacaagaaag atggatccaa aatttgggtc gcaatcaatg ccagggcagt
    18181 acggaacggt aatggttcaa tagatttctt tgaaggcttt catcaggaca tcacccgccg
    18241 taagcgggcg gaaaatgatc tcaagaatac ggtggagagt ttgaagaaga ctctcgatgg
    18301 gacagtacat gccctttcta caacccttga gatgcgcgac ccatatacgg caggccatca
    18361 gaagcgcgtt gcattgattg cctgtgaaat agcggaagaa atgggtttgc cagaggaaca
    18421 aatagaaggt atcagggtaa tgggttttct ccatgacatc ggtaagatca tggtgccagc
    18481 agaaattctc agcaagcccg gcaaaatcaa tgagtatgaa ttccacatta tcaaggccca
    18541 ttctcaggta ggttatgata tcctgaaagg tatcgaactg ccatggcctg ttgccctcgc
    18601 caccattcag catcatgaaa gaattgatgg ttcaggttat ccacagggtc tcaaaggaga
    18661 tgagattatt ctcgaagcaa aaattctcgc tgttgctgat gtccttgaag cgatggcttc
    18721 ccataggccc tatcgacctg ctctcgggct tgacaaagcc ttggaagaaa ttcgcgagaa
    18781 aaagggtgta tattacgacc ccgtagtagt cgacgcctgc cttagactat tcgtggacaa
    18841 gggtttccgg tacgagtaaa ttcacatatt atttgatctg gtttttttgc tttgaatcat
    18901 tattcaaccg ttatttcgat tatgttgtgg tctcccatta tggcgatact ggcttcctga
    18961 gggcctcgat ctttaagcca ccccttgtca aataattccg gcacagtagc tgcaagtata
    19021 ttgagatcta ctttggcgct tccacacgac aaatttgata cccagggcat attctcaaga
    19081 tcgccttcga attcaataat caacttccgg tcatccggtc cagtatcaga aacgtccgca
    19141 ttaaccttgg ggcgccattg aactttcccc gtttccatca ttattgccat ataccatgca
    19201 ggcatcggaa acctcctttc catctatttg ccacgagatt aatctatgta tgctttgagc
    19261 ttctcgtcaa ccctcaggga tagttgcgct tttcgcttat gcgtctcaag ccaatacggc
    19321 aatagtcaaa acccgccctt tttacgccat tgactggatc aaaccgtaag ccctttcaaa
    19381 atatccatgt gacgatgcac ctcccgggac gtgctcatgc aagaggctgc acacaggatt
    19441 gcnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    19501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nncaagatag tagcaacaag
    19561 acctgctaag acaacgagga ctaccaagcc ggttattccg ccctcatctc cctaggcatc
    19621 agcagcctca ccgttaccac gtgccctgct ggaagcccaa ttcgtgtttg aaaaaaccag
    19681 cattcagtaa cagccaagaa atgtgcatca agagaacccg atactccatc tccagagaac
    19741 tcatctcgct accccgaata gccgaaacgc tttcgccggc agaactacca gaggagatcg
    19801 ggatatcagg ggtaccagcc agatcagtca aatttgactt gaccgtcctt ttgagaaaga
    19861 tgtacaaaag cctacctttg atacatatga agaaaatgat ataggagaac atggccacaa
    19921 agtcatatga ggaccaggtt atcaacatgc atcatcaaaa gaagtttgag atgggaacgc
    19981 ctgaaaatct gataccaggg agaattatag ccagatgaat tagtctaaac gtaatctgac
    20041 attcctccga taaaagaaaa cagatcaagg aggaatgaca cagataacaa cgcaggaaaa
    20101 agccatcaag aacaaagcag gactcttata gcttgcaagg gagctgggga acgtctccag
    20161 gacatgcaag ctattcggct actcacggga cagctcctac cggttcaaag aactttatga
    20221 ccggggcaga tagttggcct tgcaggagct cacccgatga aatccctgcc ccaagaacag
    20281 agtagccatg gaggtagagg accgggtatg tctgatggct attgagaaac cggcctgggg
    20341 acaacttcgt gtttccaatg aactgaggcg ccagggcgtc tttgtctctc cgtccggtgt
    20401 ccggtgtgtc tggcagcacc atgacctcaa gaccttcaaa aagcgcctca aggccctcga
    20461 ggcgaaggtc gtccaggaca acatcatcct cacggaagac caactcaaag cgctggaaaa
    20521 ggcaaaggac gagaaggaag ctcacggaga gatcgaaacc taacatccgg gttacctggg
    20581 ctcccaggat accttctatg tgggcaatct gaaggatgtc ggccagatct accagcagac
    20641 cttcatcgat acctacgcca cggtggcaat ggccaagctc tatgacagga aaaatgccct
    20701 tgttgctgcg tatctgctga aggaccgcgt cgttcctttc ttcgaagaac acgatatccc
    20761 tctcttaaga atcctcaccg acagagggta tgaatactgc ggcaacagag aataccatga
    20821 atacgagctc tatctcggta tagaaggaat cgaacataca aaaacggaga caaagtcacc
    20881 acaaacgaat ggcatctgtg aacagttcca gcggaccgtc ctgaacgaat tctatcaggt
    20941 ggcattcaga accaaggtct acaaatccat tgaagaactc cagagagacc ttgatgcatg
    21001 gctaaaacag tataatgaac aacgacccca ttccgggagg tactgctacg ggaagacacc
    21061 catgcagacc tttctggacg ctcttcccat gattagggag aaaatgatcg ggtatgagaa
    21121 gacagcatga atgaaaggag tgtcaggaaa agtattgact aatacacgat gccttataga
    21181 gggggacata tgacgacaag aaaaagctgt catatccaag agactcaaaa caactctgtt
    21241 cgattcttga ggctgcac
//
LOCUS       DAOOMC010000037        20944 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdus sp. isolate AMR_MDS_2685
            NODE_2500_length_20944_cov_6.337163, whole genome shotgun sequence.
ACCESSION   DAOOMC010000037 DAOOMC010000000
VERSION     DAOOMC010000037.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864760
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdus sp. (wastewater metagenome)
  ORGANISM  Syntrophorhabdus sp.
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae; Syntrophorhabdus.
REFERENCE   1  (bases 1 to 20944)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20944)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,729
            Genes (coding)                    :: 2,708
            CDSs (with protein)               :: 2,708
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 7 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20944
                     /organism="Syntrophorhabdus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2500_length_20944_cov_6.337163"
                     /isolate="AMR_MDS_2685"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2699756"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..376)
                     /locus_tag="PK125_05335"
     CDS             complement(<1..376)
                     /locus_tag="PK125_05335"
                     /inference="COORDINATES: protein motif:HMM:NF025865.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HPB37562.1"
                     /translation="MIDLLRRRRSIRKYEKKAIGKELLDDLKEALLRCPSSRGINPWI
                     FIFVDDARLLDQLAMVKEHGSSFLKNAALGIVVCGDETKSDVWVEDCSIASIVVQLVA
                     QSSGLGSCWIQIRKRVHSPDMTA"
     gene            complement(626..2110)
                     /locus_tag="PK125_05340"
     CDS             complement(626..2110)
                     /locus_tag="PK125_05340"
                     /inference="COORDINATES: protein motif:HMM:NF014425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HPB37563.1"
                     /translation="MTNGTQNTTLAFFLIVILACVAAFSFQGRRGLYETTEGRYAEAA
                     REMVETGNYLIPTLDYKPHWTKPPLAYWGIATGIILFGQNEWGVRFSNSLSFFITTII
                     IIIIGSLLWELRTGLVAGLIYLSSPFPFFGANAVSTDTLLTLWEMLAVLCYLEAYRAD
                     ISKRKTIWITFMWFIFGLGFLTKGPPSLLPLLPVMVWNYTRRNRVAIFKGFGILLFLL
                     TGFSWFVLVTIKNPHLAAYFLKKELVDRVSSGAVHNSEWYSPFTVYAPVLLGGQGAWL
                     YFSTRSLWRCVQSGPRKIVQFIKDKEEVLFIIMWIIIPLGIFSISKSRLELYILPLYG
                     PISLLLARWIVSYENILWKRLVTIGAISVIILAVLKLGIAQFPNRNNMQQVDMIAREI
                     GGKNIEYFVFEEDKLFGMQFYLNGKMQRVTTTGQEPWSDTSIDDLLSSLRNDKTTAGY
                     LILSSMKKVRNIEAALSESGLEVEKTGNKHWIWFKVNQKHGESD"
     gene            complement(2103..2372)
                     /locus_tag="PK125_05345"
     CDS             complement(2103..2372)
                     /locus_tag="PK125_05345"
                     /inference="COORDINATES: protein motif:HMM:NF019214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase N-terminal
                     domain-containing protein"
                     /protein_id="HPB37564.1"
                     /translation="MNTFWLVLGFVAQGLFSARFLVQWIASERARKSVMPEVFWHLSI
                     VGTILLLAYAIYRRDPVFILGQSAGLIVYVRNLYLIWREKRLTID"
     gene            complement(2369..3118)
                     /locus_tag="PK125_05350"
     CDS             complement(2369..3118)
                     /locus_tag="PK125_05350"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HPB37565.1"
                     /translation="MQVAVSIIIPVKDEGENIDILGNEINMAMENVTLPWECIWIDDG
                     STDTTVGELKKLSACDARHRLVILARNFGQSAALSVGFQNATGDILVTLDGDGQNDPA
                     DIPRLIEKLQKHGADMVNGVRQKRHDSFIRKISSRIANNFRDWVTGDSVTDVGCSVRV
                     FKRECVKGIPVFKGMHRFLPTLVRMQGYTHIMESLVNHRPRVRGKTKYGIQNRLWVGL
                     ADLAAVKWMQSRLVFPEVQSMTSTTHREGEE"
     gene            complement(4047..4847)
                     /locus_tag="PK125_05355"
     CDS             complement(4047..4847)
                     /locus_tag="PK125_05355"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HPB37566.1"
                     /translation="MKDKPTPLIEFKNITVIKGGYNKIIDSVSVTIHEGENVAILGPN
                     GAGKSSFIKTITRDYYPLPGRKNYVFKIWGKENWNIFSLRKRLGIVTNSLQHVHTSEI
                     SGMDVVLSGFFSSVGLWHQKVTAKMKKKAMEALEFMEIAHLGECKITEMSSGEARRFL
                     IARALVHEPKALILDEPTNSLDLHALHILRKTLRKIAKAGTSIILVTQSLQDIIPEIN
                     RVILMKRGKFVCDGTKDAVLTSKNMSRLFDVPVVDMINKDGYYYVFGY"
     gene            5103..5363
                     /locus_tag="PK125_05360"
     CDS             5103..5363
                     /locus_tag="PK125_05360"
                     /inference="COORDINATES: protein motif:HMM:NF024634.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HPB37567.1"
                     /translation="MADLRRKKEPPKIDIYKAWCKGCGICASFCPTGALARDEAGYPY
                     VKEPEKCISCGWCEIRCPDFAITVKHKGDDEKARGIREEAIQ"
     gene            5320..6483
                     /locus_tag="PK125_05365"
     CDS             5320..6483
                     /locus_tag="PK125_05365"
                     /inference="COORDINATES: protein motif:HMM:NF006412.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HPB37568.1"
                     /translation="MMKRREEYVKKRFNKEKLLQGNEAVVEGALYAGCRFFAGYPITP
                     ATEISEVMSQRLPAVDGTFIQMEDEIASMGAVIGASLAGAKSMTATSGPGFSLMQENI
                     GYACIAEVPCVVVNVMRGGPSTGLPTSPAQGDIMQARWGTHGDHPAIALCPSTIRECY
                     ELTIKAFNFSEKYRNPVILLLDEVVAHMREKITLGDDRIDIFNRVKPTVPPEWYIPYE
                     DTPFGIPPMANFGEGYRYHVTGLTHDVRGFPTSRPDEIDPFIRRIFRKISQNFSDLQI
                     GESFMTEDADVSVIAYGCVARSAKRAVIDARERGIKAGLLKINTIWPFMRIQVEKVLK
                     TSHCVIVPEMNMGQISREVKRVNKGDAKVFTINKVDSTIITPTEIFNRIREVC"
     gene            6483..7319
                     /locus_tag="PK125_05370"
     CDS             6483..7319
                     /locus_tag="PK125_05370"
                     /inference="COORDINATES: protein motif:HMM:NF014794.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:ferredoxin oxidoreductase subunit
                     beta"
                     /protein_id="HPB37569.1"
                     /translation="MAGVTELIHKYLRHDKKFPHVWCPGCGIGIMLGALIRAIDQCGF
                     AKDEIVLVAGIGCTGRLPVYVDFNTLHTTHGRALTFATGVKLANPKLKVIVVMGDGDA
                     VAIGGNHFIHAARRNIDLTAIILNNSVYGMTGGQFSPTTPYGMRTTTTMFSNVEQAFK
                     ISELAVTAGAVFVARGSVYHAKMLDGLLEKAFMKNGFSVVEVVSHCHTQYGRLNRMGT
                     AVEMMQWQKEHAITVEKAAGMADEELADKFKIGVLIDRELPVFQDEYDKVKARAKGIS
                     IP"
     gene            7320..7907
                     /locus_tag="PK125_05375"
     CDS             7320..7907
                     /locus_tag="PK125_05375"
                     /inference="COORDINATES: protein motif:HMM:NF013707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HPB37570.1"
                     /translation="MGNRYDIRLSGSGGQGLILMGIILAEAIGIYDGKFVAQTQSYGP
                     EARGGSSKAEVVVSDQEIDYPKALRLDLLLAMNQKSCDDYYMDLKPTGVLLVDSTFVN
                     QIPIPRAYQIPFTRIARDRFKKEMVANIIALGAISWLTPIVSVKAIEAAVLARVPRGT
                     ENLNRNALRAGISAAKKVEKTTLLTPSNFLENEDT"
     gene            7894..9069
                     /gene="sucC"
                     /locus_tag="PK125_05380"
     CDS             7894..9069
                     /gene="sucC"
                     /locus_tag="PK125_05380"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019902207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HPB37571.1"
                     /translation="MKIHEYQAKALLQSYGIPIPRGYIAKTPEDTLKAAQTIKKGPFV
                     VKAQVHTGGRGKVGGISIVESPIQAEAFAEEILGAKLVTEQSGPEGKPVHTLLIEEAL
                     LSKMEIYLGIVIDRTKGCPVILASAQGGMEIEEVVKEHPDMVITETVHPSVGLRPFQL
                     NRICYGIHLDPKLMKKMTAVIHNLYRLFLDKDCSLAEINPLAVKNDGEIVAVDAKLNF
                     DDNALFRHPEIAALRDLSQENPLEIEASRYNLNYIKLSGNVGCMVNGAGLAMATMDLI
                     KLSGAEPANFLDVGGGATTEMVREGFKLLISDKEVKVIFINIFGGILRCDILAKGVVE
                     AASELNVTLPVVIRLEGTNVTEGRKILTESGLQFAVASSLGDAAAKVTLALRDSFKL"
     gene            9126..10019
                     /gene="sucD"
                     /locus_tag="PK125_05385"
     CDS             9126..10019
                     /gene="sucD"
                     /locus_tag="PK125_05385"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018287377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HPB37572.1"
                     /translation="MSILIDQDTGVVIQGITGGEGAFHTRQMKEYGTKVVAGVTPGKG
                     GQKFDDVPIFNTVREAVENTKANTSGIFVPPAFAADAIMEAVDAGLEIIVCLTEGIPT
                     LDMIAVKQFMKGSNSKLIGPNTPGIISPGKCKVGVMAGYIHTPGRVGVISRSGTLTYE
                     VVDQLTKLSIGQSSCVGIGGDPVIGMTFVETLNLFEKDKDTDIVVIIGEIGGHAEEEA
                     ADFFRKNMSKPVVAFIAGVTAPPGRRMGHAGAIVTGGQGGAREKIDILEKAGITVVKN
                     PSEIGETVARVLAVRKSAYRL"
     gene            10127..10942
                     /locus_tag="PK125_05390"
     CDS             10127..10942
                     /locus_tag="PK125_05390"
                     /inference="COORDINATES: protein motif:HMM:NF014670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="creatininase family protein"
                     /protein_id="HPB37573.1"
                     /translation="MDYSIFSGTMADMTYLQIEEAARKKLPVLFPIGVIEEHGPHMCL
                     GTDVYLAYNLAKDVQKGLRVFQVESIIAPAYYWGINAAMNGFAGSFSVKPETMVSVLF
                     DLLKCLKNWSFEYVFLLNVHGDFEHNLAMTEAVRKAYDELRLRAYSIVPEFFRQRANM
                     SGKEPYIILYDVEFNKSSPYLDIHAGAFETSLMVEKFPNLVDIDTAQSLPSSETTIEG
                     LKKWLKGGSGAREVTPLGYLGDPSAIDVRAANESNEKLVNEIVKAINTTLTKE"
     gene            11107..15324
                     /locus_tag="PK125_05395"
     CDS             11107..15324
                     /locus_tag="PK125_05395"
                     /inference="COORDINATES: protein motif:HMM:NF013491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HPB37574.1"
                     /translation="MDPNRIGLQSSALQVNLERTAAQVEIPTEYLPLLEVVKNHYGLF
                     KKTHELLIELNHPFVNWNYVLQQLRTISIGDFYEYNRHENGLQAMKIILQIYESVMQR
                     AVNEELRENAVRYLFDYLDTIISNSGEYLVRNIVLVPQVIESLLAIFDSEGFILKKSS
                     GYFKRMLKMLFEHGVQIPLDSLTMLLYKVFKSTYQFWLMQPDPSGWLHEEDETEDAIK
                     VFGELIFPLSHEHLRSLIEKVENINASLLTQPEKACVAYTDLPDYWQIVNGYLMIADE
                     LEKSSVFAGRQNFVKLDFLFNIMGMPSLSDIHASSLREINLSLRMAFRENKNENLEEF
                     VRRVFALLKKSAGRHEFRSAVIDCITTIAKEVFSQNKHPLVDTFIEETILFGFQYPEV
                     KGSTAEWQVQVNPAHILNVRSWLGIIAMKPRWTKRLLSALIINLKLGGIFVRDTDLIQ
                     KDISALLNSDIGPAYNLTKQLLRIFPVYFSEIGAEGELRDISTKVDELSFRNDKLINF
                     LRKQSHVESNSLLVGFVENAFLYWYSGDKDILKKDLPNEVYDQIANSGEYFDGLHSIF
                     KYIMVKANNDPAILLSWDRAKAHKEIYGIRGVSLRDKERALLMIRFYQLLHKKYNPQH
                     TELLKDLESNYVFDRSQVKALQRSLKRKNFTRSLSIILDFLGLLKEKILSPQKTEYFE
                     NIYHKRHIAAGIPSMYGTYHEEKFEAVGLSLRLESLATFLFEELIRSMNLKFITKSTL
                     IMIHDCLGLYIRALELEGIATEGLVAKIKYVTSALQVKMFSVDQYIDIFRFISKGIQH
                     IIRDYYVDAHRLNLPIIVKQLIEAQNDGQHQAQEQRQEEIIYQYSENFVRGMISTAFG
                     LQVLDNLVNGVIETLNAELERFKDNKQILNLVMAYSPDLAISSIYQRNKQLDNQILLG
                     NKGYFLKELASFGFRVPPGFILTTEIFRGYDAVVGYKYIFKDLTIRINREIAELEKIM
                     GSRFGDPRNPLLLSVRSGATISLPGMMNSFLNVGINEDIAEGLGRRRGHQWAAWDSYR
                     RFLQTWGMYQGLKRDFFDDIMNSFKTKYDVKKKIQFKPDQMKRIALAYKGRMAEKGIN
                     VADNPFVQLREAILQVFASWYSEQAKIYRHQMRLSDEWGTAVIVQAMVFGNLNANSGS
                     GVIFTRNPKGTSANVSLSGDFIFGVQGDDIVSGLVITYPISEKQRLAEKRAFEISLEN
                     KFPEIYNELVRLSEILIYEKGFNHQEIEFTFENSTPDKLYILQTRDMVQRQTNKVKRF
                     KDTEALHKSYLGTGIGVSGGALTGKAVYSEKDIKRFRKSEPETPLILIRPDTVPDDVG
                     ILLQVEGLLTARGGGTSHAAVTIPQLDKVGVVGFNKLQVYETDGWSLVDGLKIKSGDF
                     IGIDGWSGAVYLGKNESESEESYNITL"
     gene            complement(15493..16770)
                     /locus_tag="PK125_05400"
     CDS             complement(15493..16770)
                     /locus_tag="PK125_05400"
                     /inference="COORDINATES: protein motif:HMM:NF014818.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyceraldehyde 3-phosphate dehydrogenase
                     NAD-binding domain-containing protein"
                     /protein_id="HPB37575.1"
                     /translation="MARAKVFENRPPTLGINSLGRIGKLTLWHHVGRKYFKEIIVNLG
                     RESGTGLQSIAQMIEKDATYGLLHRFLYGIKSERLIEIKDEKKGKLVIDGIPVTILRE
                     ARNPVDIPWRDYNVDLVVDCTGKFKDPTVQPDDSKGSIRGHLVKGAKAVINSSAFKIK
                     NKALSTPADCVTLIYGINHSAFDQKKHQIVSAASCTTTGLAHMVKPLLENEKTNTILT
                     ASMSTVHAVTNSQSVLDSVPKAGEKDLRKNRSILNNIILTSTNAAEALGQVIPEVKNI
                     GFMADSIRIPTNTLSLIILNVTFQTKMNADGTSAAINTKAINDIYKKTYESNPEHLLI
                     YTTEQNVSTDLIGINAAVIIEGQFNHSRTAFINVDLTQIPNFSAKALQVMESKTLRVP
                     VVHAKIFGWYDNEYGSYTNRMGDLTVHVHKNLL"
     gene            17076..17912
                     /gene="amrB"
                     /locus_tag="PK125_05405"
     CDS             17076..17912
                     /gene="amrB"
                     /locus_tag="PK125_05405"
                     /inference="COORDINATES: protein motif:HMM:TIGR04336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein B"
                     /protein_id="HPB37576.1"
                     /translation="MAFIREPSVSGTFYPDNPKILKKTIEDYLQNVIFEPSNKEVIGL
                     LSPHAGYIYSGQVAAYGYKAIMGSHYDTVIVIAPSHRSHFEGSATIVKGGYRTPLGVV
                     QIDEELAGEIVSLKTVVWPDIDAHRDEHSLEVQVPFLQVILKNFRLLPLILGSQEPSV
                     FVALVDTLYRSIKKTNRKVLVVGSTDLSHYYPYAEAVRLDTVVKQRLQSYDIEGLQDD
                     FYKGTCEACGTGPILTTMMLSKKLGASYAKVLKYANSGDVSGDRSGVVGYISCIFTIN
                     NQ"
     gene            17930..18463
                     /gene="amrA"
                     /locus_tag="PK125_05410"
     CDS             17930..18463
                     /gene="amrA"
                     /locus_tag="PK125_05410"
                     /inference="COORDINATES: protein motif:HMM:TIGR00296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein A"
                     /protein_id="HPB37577.1"
                     /translation="MNLSNEEKKKLKDLARDAIECALFGKEQCEIELSEKLKEKAGAF
                     VTLKSKGELRGCIGHISASMPLSDVIEKMAIESAFHDPRFCALRKDEWKDIDIEISVI
                     SPMRQIKDIEEIEVGVHGLYIVKGGYSGLLLPQVATEYHWDRKTFLEYTCYKAGLSKD
                     SWKSNDVKIYIFSAEVF"
     gene            18513..18722
                     /locus_tag="PK125_05415"
     CDS             18513..18722
                     /locus_tag="PK125_05415"
                     /inference="COORDINATES: protein motif:HMM:TIGR03980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1858 domain-containing protein"
                     /protein_id="HPB37578.1"
                     /translation="MINGSMLIEDAVSKYPETIPVFEKFGFGCLGCQAALFESIEQGA
                     KIHGINVEALITSLNKAIAKRCSIT"
     gene            18755..19366
                     /locus_tag="PK125_05420"
     CDS             18755..19366
                     /locus_tag="PK125_05420"
                     /inference="COORDINATES: protein motif:HMM:NF024214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HPB37579.1"
                     /translation="MKMFLAIILAAGTSSRLGFNKLTVFIDGESVIRRSVTPFLMAEI
                     DRILVVTGNDSENVKEQLEGLPVSFIYNPCYKKGMSSSIKTVTPFLEGSEAVLFHLGD
                     KPFVSSHSIKTVLNTFRAGKAKIVLPRFDGKTGHPVMISTKLLEDEIGNLHGDRGLRE
                     VIEKYKQDVVFIEGDTGNLFDIDTIQVLDQLRERGYTIEENKR"
     gene            19347..20363
                     /locus_tag="PK125_05425"
     CDS             19347..20363
                     /locus_tag="PK125_05425"
                     /inference="COORDINATES: protein motif:HMM:NF013183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-binding protein"
                     /protein_id="HPB37580.1"
                     /translation="MKKISVEKALGMTLAHDITEIVPGKKKDVAFSRGKIIEKGDIER
                     LLDLGKRYLYVFEGPAKGVHEDDASTRIAQAILGEYMEYTSPKEGKVTIKTTENGLFY
                     VNAQHLFAINKIKNVLLSTLPNRHLVKKGDIIAATRIIPLYIDEKSLKRVEKIGEGRV
                     ISVSPFKSLNIGLVITGSEVYTGRVQDGSSLVENKLRNYGLTVAGKIIVPDEISMIKD
                     AILSLFNSGVDLVLTTAGLSVDPDDVTKEGIEATGAETLFYGTPVFPGAMFLVARLRG
                     KYILGAPACVYYNRNTVLDIILTRLLAGERMHKNDMVKLSYGGLCLQCDECHYPTCFF
                     GKGL"
     gene            20360..>20944
                     /gene="yqeB"
                     /locus_tag="PK125_05430"
     CDS             20360..>20944
                     /gene="yqeB"
                     /locus_tag="PK125_05430"
                     /inference="COORDINATES: protein motif:HMM:TIGR03309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium-dependent molybdenum cofactor
                     biosynthesis protein YqeB"
                     /protein_id="HPB37581.1"
                     /translation="MMKNSSSFIVQHSAKNKKREKKIHDLIVAIKGAGEMATGIAHRL
                     YEANIRRIVMTEIPEPITVRRTVAFSEVVYEGEVIVEGVHAKRAVNVDEVNDIWQHDM
                     IAVVVDPKCTTVSVLKPDIVIDAIMAKKNLGTKRTEAPFVIGVGPGFIAPDDVHAVVE
                     SNRGHNLGRVFYDGAAEPHTGVPGPTMGYTSERVL"
BASE COUNT         6147 a         4087 c         4836 g         5874 t
ORIGIN      
        1 cagctgtcat gtcaggggaa tggaccctct ttctgatttg gatccaacag ctgccaagac
       61 ctgaagattg ggctaccagt tgtactacta ttgatgcaat tgaacagtcc tctacccata
      121 cgtcagattt tgtctcatct ccacaaacta caatgccaag ggctgcgttt ttgaggaagg
      181 atgatccgtg ctctttaacc attgccagct ggtccaaaag cctggcatca tccacaaaga
      241 taaagatcca tggattgatg ccccgcgaag atgggcaacg aagcagggcc tctttaaggt
      301 catcgagtag ctccttaccg atagctttct tttcgtattt ccttatactt cgtcgccttc
      361 gtaacaaatc gatcatattt ccctccctca ttgatcgtct cgctctctct attgcttact
      421 cagccgctgt ggttcttaca tcgtccttct aatgtcaaat gctgcagagt tctcctgtca
      481 gtctattatt ttcctgtacc ctgggctggt ttgcaagtaa attccgagaa caagataaat
      541 ctggtccaaa aggatacaga tttatacctt cagggccggt tacggtcaaa tctatgtata
      601 acccaaaaga ctaaagcacg agaagtcaat cagattcacc atgtttttga tttaccttaa
      661 accatatcca atgcttattt ccggtttttt caacttccaa gcctgattct gacagggcag
      721 cctcgatatt tcgcaccttt ttcatactcg aaaggataag gtagcctgct gttgtcttat
      781 catttctcaa ggatgacaat agatcatcaa tgcttgtatc agaccacggt tcttgtcctg
      841 tcgttgtgac cctttgcatt ttgccgttta ggtaaaactg cattccgaaa agtttgtctt
      901 cctcgaagac aaaatattca atgtttttac caccaatttc acgagcaatc atgtcaacct
      961 gctgcatgtt gttcctgttt ggaaattgtg caatgcccaa ttttagaaca gctaagatga
     1021 taacgcttat tgctccaatt gttaccaacc ttttccataa gatgttttca taactgacaa
     1081 tccatcgtgc taggagcagc gaaatggggc catacagggg caagatatag agctctaaac
     1141 gactcttaga tatggaaaaa atacccagag gaatgataat ccacataatt ataaaaagaa
     1201 cctcttcttt atctttaata aactgcacaa tttttcgtgg gcctgactgt acacatctcc
     1261 agaggctacg ggtgctgaaa taaagccatg ccccttgccc ccccaataac acgggggcat
     1321 aaacggtaaa tggactgtac cattcactgt tatgaactgc cccagaagat accctatcta
     1381 ccaactcttt cttgagaaaa tatgcagcta agtgaggatt ctttatagtt acgagtacga
     1441 accaggaaaa accggtcaga agaaataata aaatcccgaa acctttaaaa atggccaccc
     1501 tgtttctcct tgtgtaattc cagaccataa caggaaggag gggaagtaat gagggaggac
     1561 ccttggttag aaagcccaaa ccaaaaataa accacatgaa agttatccag atggttttcc
     1621 tcttcgatat atctgccctg tatgcctcca aataacagag gactgccagc atctcccaca
     1681 atgtgaggag tgtgtcagta gacacagcgt ttgcaccgaa aaaggggaaa ggagaggaga
     1741 gataaatgag tcctgcaacg agacctgttc tcagctccca aagaagggag ccaataatta
     1801 ttataataat agtagttatg aaaaaagata aagaattaga gaaacgtact ccccattcgt
     1861 tttggccgaa aagtattatt cctgttgcga taccccaata ggcaagcggt ggttttgtcc
     1921 agtgtggctt ataatccaat gtgggaatga ggtagttccc tgtttccacc atttctctgg
     1981 ctgcctccgc ataacggcct tctgtggttt cataaagccc ccggcgaccc tggaaagaaa
     2041 atgccgcaac gcaagcaagg ataacaataa gaaaaaaagc cagagtagtg ttttgtgtcc
     2101 cattagtcaa tggttaacct cttttctcgc catataagat agaggtttct cacatagaca
     2161 atcaaaccgg cagactggcc gagaataaag acggggtccc ttcgatagat tgcataagcg
     2221 aggagaagaa ttgtaccaac aatgctcaga tgccagaata cctcgggcat aacgcttttt
     2281 cgtgcccgtt cactggctat ccactgaacc aggaatcttg ccgaaaaaag gccctgggca
     2341 acaaatccca gaaccaacca gaacgtattc attcctctcc ctccctgtgg gtagtgcttg
     2401 tcatggattg aacttccgga aacactaatc tgctctgcat ccactttact gcagcaagat
     2461 ctgcaagacc aacccaaaga cggttttgaa ttccatattt tgttttgcct cttaccctcg
     2521 gacgatggtt aaccagtgat tccataatgt gtgtatagcc ttgcatcctg accaatgttg
     2581 gtaaaaaccg gtgcatgcct ttgaagaccg gtattccttt tacgcattca cgcttaaaaa
     2641 ccctaacaga gcaacccaca tcggtaacgc tgtcgccggt aacccaatcc ctaaagttgt
     2701 tggcaattct cgatgatatt ttgcggataa aagaatcatg ccttttctgg cgaacaccat
     2761 tcaccatatc agcgccatgt ttttgtagtt tctcaatgag gcgcggaata tcagcagggt
     2821 cgttctgtcc gtcaccgtca agagttacga ggatatctcc tgtggcattc tggaatccta
     2881 ctgaaagagc agctgattgc ccgaaatttc gagcaagaat gactaaacgg tggcgggcgt
     2941 cacatgcgct gagttttttt agttccccta cagttgtatc tgttgaaccg tcgtcaatcc
     3001 atatacattc ccagggcaag gtaacattct ccattgccat atttatttca tttccgagaa
     3061 tatctatgtt ctctccctca tccttgacag gtattattat acttacagca acttgcatcg
     3121 ttagatatgg cactccgcag agttaatcga acagcagaaa catgggcgta aacattcgat
     3181 gaatctttat attacactat cacataaagc atattgaagg aaagatagaa tacaaaaaac
     3241 acaatgtttg atagaatggt gagagtggaa actcgatcat tcacattccc cggggcttgc
     3301 ttcaggaatt ctgcacggga gagtctgata aatcgagggt ttctcagggc aatagggatt
     3361 caattacata cgtggcaggt ctgttgagga ttatttgatc atattctttt ccaggcagga
     3421 gcagtgatat cttcgattca tcagattgct gttgctattg aacagcggac aacttgtatg
     3481 aatgaaatct cagacatcag gcagtatttt cattacaata cgacatgtca acgtgaatcc
     3541 atgacaatac cgatgctata ctgtaccacg gtataatctc cgaggatttg gaaagattgg
     3601 tgagttaact ccaagttcaa aaagttgaga atatagatac aggttgtgat ctactgacac
     3661 tttacgttct ggtttcattc aaaggtaacc atgttgacag gcaatgaaca aatccttaca
     3721 atcagtgtat gacagccaaa gattatgtag tggagaccca aaggaaattc tgtataaact
     3781 gcgacaacaa gcacgggtgc aagtcgggca ctccaccctg tgtcggtgaa atgacaaaaa
     3841 ataatgtcag gggatggtca ggaaaaaaat attttatgga tttgaacaag gtttatcggt
     3901 gcaaagactg ctcctttttc cgctcgtgct ggacgatgga gaagtatgat agagcgctta
     3961 ataagtgata aatacccaca tcatattcac tggccatatt ctttttttga tggcacgatt
     4021 atggaagtga ccagtgtttg ttatgcttaa tatccaaaga cataataata tccatcttta
     4081 ttaatcatat ccaccacagg aacatcaaag agcctgctca tgtttttact tgtcagcaca
     4141 gcatctttgg ttccatcaca gacaaattta cctctcttca tgagtatgac acgatttatt
     4201 tctggtatga tatcctggag gctctgtgtg acaagaataa tactcgtacc agcttttgct
     4261 attttgcgca gagtttttct gagtatatga agagcgtgga gatcaaggct attagtcggt
     4321 tcatccagga tgagggcctt tggttcgtga acgagagccc ttgcaataag aaacctacgt
     4381 gcttcacccg atgacatctc agtgattttg cactctccca gatgggctat ttccataaac
     4441 tcaagggctt ccatggcttt tttcttcatc tttgcggtta ctttctggtg ccacagacca
     4501 acactgctga aaaatcccga taacacaaca tccattccag aaatctcgct ggtatgaaca
     4561 tgttgcaggc tattggtcac gatccctaat cgtttacgaa gactgaaaat attccagttt
     4621 tctttacccc aaatcttgaa tacgtaattt ttgcggcctg gtaaaggata ataatctcga
     4681 gttatggttt taataaatga tgatttacca gcaccgttcg gaccaaggat tgctacattt
     4741 tctccttcat ggattgtaac agagactgaa tcgatgattt tgttatagcc ccccttgata
     4801 acagtgatgt tcttgaattc aatcagaggt gtcggtttat ctttcatctt tgtggaaaat
     4861 aaaaaatggg catagtacga atcagaaggt agatcagatt tttatgtacc ataagtttcc
     4921 catcgtttcc cttattttga cttattttcc cgcaatcgca gccttctttc aatggaaaag
     4981 ttaaaaatga aaattacaat gggtttttta gtgtcaagca tgaagatgta tgttttcaga
     5041 ttaatcaaaa agcttgacaa caacggagaa tcattttagc tttactcgag agatgaaaat
     5101 aaatggctga tttgagacgg aaaaaagaac cccctaagat agacatttac aaagcgtggt
     5161 gtaaaggctg tggaatctgc gcttcttttt gtccgactgg agcgcttgcg cgtgatgaag
     5221 cgggttatcc ctatgtgaaa gagccagaaa aatgtatcag ctgtggatgg tgtgaaatca
     5281 ggtgtccaga ttttgcgata acggtgaagc ataaaggcga tgatgaaaag gcgcgaggaa
     5341 tacgtgaaga agcgattcaa taaggagaaa ctgctccagg gcaatgaagc tgttgtagag
     5401 ggagcgctct atgcaggctg tagattcttt gctggttacc ccataacacc tgccactgaa
     5461 atttcagagg taatgtcaca acgactgccg gcggttgatg ggacattcat ccagatggag
     5521 gatgagattg ccagcatggg tgctgtgatt ggtgcatcac ttgctggagc aaaatcaatg
     5581 acagccacaa gtggtcctgg tttttctctc atgcaggaaa atattggata tgcatgcatt
     5641 gctgaggttc catgtgttgt ggtaaatgtc atgcgtggcg gccccagcac tggtcttccc
     5701 acgtcacctg cccagggaga tataatgcag gctaggtggg gtacccatgg agatcacccg
     5761 gccatagctc tctgcccatc cactatcagg gagtgctacg agcttacgat aaaggccttc
     5821 aatttctcgg aaaaatatag aaatcctgtg atactgcttc tcgatgaggt agttgcccat
     5881 atgagagaaa aaataaccct cggcgatgat agaattgaca tcttcaatag agttaaacct
     5941 actgtgccac cagaatggta tattccgtat gaagatacgc ctttcggtat tcctcccatg
     6001 gcaaattttg gtgaaggcta tcgttaccat gtaacaggtt taacacatga cgtgagaggt
     6061 tttcccacct caaggccgga tgagatcgac ccattcatca gaagaatctt caggaagatt
     6121 agccagaact ttagtgatct tcagataggt gaatccttta tgactgaaga tgctgatgtt
     6181 tcagtaattg catacggatg tgttgcacgg tctgcaaaaa gggcggtcat tgatgcccgc
     6241 gagcggggga taaaggcagg tctcttaaaa ataaacacga tttggccctt catgagaatc
     6301 caggttgaaa aagtgttaaa aacatctcat tgcgttattg ttccggaaat gaacatgggc
     6361 cagatttctc gggaggttaa aagggtaaac aaaggcgatg caaaagtttt tactattaat
     6421 aaggtggata gcaccataat aaccccgaca gagatattta atcgaatcag ggaggtatgt
     6481 tgatggcagg agttaccgaa ctgattcaca agtatcttag acatgataag aagtttcctc
     6541 atgtatggtg tcctggttgc ggaataggga ttatgcttgg cgccctgatt cgggcgattg
     6601 accaatgcgg ttttgcaaaa gacgaaatag tgcttgtagc gggcatcggt tgtactggac
     6661 gtctacctgt gtatgtagat ttcaatacgc ttcatacgac ccatgggaga gccctgacat
     6721 ttgctaccgg cgtgaaactc gcaaatccta aattgaaggt tatagtggtg atgggagacg
     6781 gtgatgcggt tgcaataggc gggaatcatt ttattcatgc cgcaagaaga aatatagatc
     6841 ttaccgccat tatcctcaac aacagcgtat acggaatgac aggcggacaa ttctctccaa
     6901 caacgcctta tggtatgaga accacaacaa cgatgttttc caatgtggag caggcgttca
     6961 aaatttcaga gcttgcagta actgctgggg ctgtgtttgt tgcccgggga agtgtctacc
     7021 acgcaaagat gttagacggg cttcttgaaa aggcatttat gaagaatgga ttctctgttg
     7081 ttgaagttgt ttctcactgt catacccagt atggcagatt gaatcgtatg ggaacagctg
     7141 tagagatgat gcaatggcaa aaagaacatg caatcaccgt agagaaagcg gcgggaatgg
     7201 ctgatgaaga acttgcagac aaattcaaga taggtgttct tatagatcga gaactccctg
     7261 tttttcagga tgaatacgac aaggtaaaag cgcgggcaaa ggggatcagc ataccgtaaa
     7321 tgggcaatcg ttacgatata cgtcttagtg gatctggtgg ccaggggctg atcctgatgg
     7381 gtattatact tgctgaggca atagggattt atgatggcaa gtttgttgct cagacccaaa
     7441 gttatggacc tgaggcgcga ggaggttcaa gtaaggcaga ggttgtcgta agtgatcaag
     7501 aaatagatta ccccaaggca ttaaggcttg accttctgct tgcgatgaat cagaaatcct
     7561 gtgatgatta ttatatggat ttaaaaccga cgggtgtctt gctggttgat tcaacctttg
     7621 taaatcagat tccaatacca agagcctatc aaataccatt tacacgaatt gcacgggaca
     7681 ggttcaagaa ggagatggtg gcaaacatta ttgcactggg cgctatttca tggttgacac
     7741 caatcgtttc tgtaaaagcc attgaagcag cagttctggc aagggtgcca agaggaacag
     7801 aaaatttgaa ccgtaacgcc ctgcgtgccg gcataagcgc cgcaaagaag gtagaaaaaa
     7861 cgacattgtt aactccgtcc aattttctgg aaaatgaaga tacatgaata tcaggcaaag
     7921 gccttgctcc aatcgtatgg catccctata ccacgcggtt acatagcgaa aacccctgaa
     7981 gatacgttaa aggcagcaca gactatcaaa aaaggaccat ttgttgtaaa ggcacaggtt
     8041 cacactggtg gccgcggaaa ggttggaggt atcagtatcg tagagagccc tatccaggca
     8101 gaagcattcg ctgaagaaat actgggagca aagttggtta cagaacaaag cggtccagaa
     8161 ggaaaaccgg ttcatacgct gctcatcgaa gaggcattgc tgtccaaaat ggagatatat
     8221 cttggcattg taatagatag gacaaaaggt tgtccggtga ttctggcaag tgcgcaggga
     8281 gggatggaga tcgaggaggt tgtaaaagaa caccctgata tggtcattac agaaactgta
     8341 catccttcgg taggtttacg accttttcag ttgaacagaa tctgctatgg gattcatctt
     8401 gatccaaagt taatgaaaaa gatgactgct gttattcata acctttaccg gcttttcctg
     8461 gacaaagact gctcacttgc cgaaataaat cctttagcgg taaagaatga tggtgaaata
     8521 gtggctgtcg atgccaagct taatttcgac gataacgccc ttttcagaca ccctgaaatt
     8581 gctgccctta gggacctttc ccaagaaaat cctctcgaga tagaagcatc cagatacaac
     8641 cttaactata ttaagctgag tggaaacgtt ggatgcatgg tcaatggtgc tggcctggcc
     8701 atggcaacca tggaccttat aaaactttct ggtgctgaac cggcgaattt tctggatgtt
     8761 ggtggagggg caaccacaga aatggttaga gaaggattca aactgcttat ttctgataaa
     8821 gaagttaagg taatctttat caatatattt ggagggatat tacgatgcga catacttgca
     8881 aaaggtgtgg ttgaagctgc cagtgaatta aatgtgacgc tgccagtcgt gattcggctt
     8941 gaaggcacaa atgttacaga agggcgtaag attcttactg agtcaggctt gcaatttgca
     9001 gttgcatcct cccttggaga cgcggcagcg aaagtgactc ttgcattgcg agacagtttt
     9061 aaattatagg tgcgggggat ttttaatgtt tttaccctgt gctttatgtg ccataaggat
     9121 tcgatatgag tatactgatt gatcaagaca caggagtcgt cattcagggt attaccggtg
     9181 gggaaggtgc atttcatacc aggcaaatga aggaatatgg aacaaaagta gttgccggcg
     9241 tgacccctgg gaagggcggt caaaagttcg atgatgttcc catttttaac actgtcagag
     9301 aagctgtgga aaatacaaaa gcgaatacat ctggtatttt tgtgcccccg gcatttgctg
     9361 cagatgcgat aatggaggct gttgatgcag gtctagaaat aattgtttgt cttacagagg
     9421 gaattcccac cctcgatatg attgctgtta agcagtttat gaaaggttca aactcaaagc
     9481 tcataggccc taacacaccg ggtattatat caccgggaaa gtgcaaagtt ggagttatgg
     9541 ctgggtatat tcatacgccg ggacgggtag gcgtgatatc gcggagtggc acccttactt
     9601 acgaagtggt cgatcaactt acgaagcttt ctattggcca atcaagctgc gttggtattg
     9661 gtggggatcc tgtaattggt atgacttttg tggaaaccct aaaccttttt gagaaggaca
     9721 aggatactga tattgttgta attataggag aaataggtgg tcatgcagaa gaggaggccg
     9781 cagacttttt tagaaagaat atgtcaaaac cggttgtagc ttttatcgcc ggtgttacgg
     9841 cgccccccgg aagaagaatg gggcatgcag gtgctattgt gacaggaggc caaggaggtg
     9901 cacgggaaaa gattgatatc ctcgagaagg ccggtatcac ggtggtcaaa aacccttctg
     9961 agattggtga aacagtagcc cgagttctgg cagtgagaaa atccgcatac agactttaaa
    10021 agagagcaca ttgtggatgg aagttctcag ttcacaggta acattgatta caataattcg
    10081 cataatccgc agaagtttta ctgaaggcca aggggtatta gattagatgg attattctat
    10141 attttcagga acaatggcag acatgaccta tctgcagatt gaggaagcag cacgaaaaaa
    10201 acttcctgtg cttttcccta ttggagttat tgaagagcat ggaccccaca tgtgccttgg
    10261 cactgacgta tatcttgcat ataacctggc aaaagacgta caaaaaggcc ttcgcgtctt
    10321 tcaagtagaa tcgattatag caccggcata ttattggggt atcaatgctg cgatgaatgg
    10381 atttgccggt tcattttcag ttaaaccaga gacaatggtc tctgttttgt tcgatctcct
    10441 aaagtgtttg aaaaattgga gttttgagta tgttttcctg ttaaatgtac atggtgattt
    10501 tgaacacaac cttgcaatga cggaagcagt aagaaaggca tatgatgaat tgaggttaag
    10561 ggcatactcc attgttcccg aattttttcg acaaagagct aacatgagtg ggaaagaacc
    10621 atacatcatt ctttatgacg tggaattcaa taagtcctcc ccatatctgg atatccatgc
    10681 cggggccttt gagacgagtc tcatggtgga aaaattcccg aatcttgtag atattgacac
    10741 ggcccaaagc cttccttcat cagagacaac tattgaaggt ctcaaaaaat ggttgaaagg
    10801 aggttctggg gccagggaag taacccctct cgggtatctt ggtgacccat cagccattga
    10861 tgtccgggcg gccaatgaat cgaatgaaaa actggtcaat gaaattgtga aagccataaa
    10921 tacaactctg acaaaagaat gaatctgctt tgtgtctgtc caggagccct tatttgaatc
    10981 tgaaatttaa tttttgggag ctaactgact agtcatcatt gtaatttttt caggcatcag
    11041 gtcttccttg caatattcag gtaaataaga ttaattaatt gtaactaact ataatacagg
    11101 tgatacatgg acccgaatag aattggttta cagtcgtcag cactccaggt taaccttgag
    11161 cgaactgcgg ctcaggttga aatccctaca gaatatcttc ccctattaga ggttgtcaaa
    11221 aatcactatg gtttgtttaa aaaaacccat gaactcctga tagaactgaa tcatcctttt
    11281 gtcaactgga attatgtcct tcaacagctg agaaccatat caattggcga tttttatgag
    11341 tacaacagac atgaaaatgg cctgcaagca atgaaaatta ttcttcagat ttacgaaagc
    11401 gttatgcaac gggccgtcaa tgaggagtta agagagaacg cagtacggta tctttttgat
    11461 tatcttgata caattatatc taatagcggt gagtatcttg taagaaatat tgtgttagtg
    11521 cctcaggtga tagaatccct cctagccatt ttcgacagtg aaggtttcat cctaaaaaag
    11581 agttctggtt atttcaagag aatgctgaag atgctttttg agcatggtgt tcaaataccc
    11641 ctggattcac ttacaatgct tttgtacaag gtgtttaaaa gcacttatca gttttggctt
    11701 atgcaaccgg atccttcagg atggctccat gaggaagatg agacagagga tgctatcaag
    11761 gtgtttggtg agcttatctt ccccctgagt catgaacacc ttcgctcact catagaaaaa
    11821 gttgaaaaca ttaacgcctc cctgttaacg cagccagaga aagcttgcgt tgcgtacacc
    11881 gatttaccgg attactggca gattgtaaac ggatacctca tgattgccga tgagcttgag
    11941 aaatcgagtg tatttgcagg gcggcagaat tttgttaagc ttgattttct cttcaatatc
    12001 atgggaatgc ctagcctttc ggacattcat gcaagctccc tgagagagat taacctttcc
    12061 ctgaggatgg ctttcaggga gaacaaaaac gagaatctcg aggaatttgt tagaagagtc
    12121 ttcgctctat taaaaaaaag tgcgggtaga cacgagttcc ggagcgctgt tattgattgc
    12181 attaccacta ttgcaaagga ggtcttcagc cagaacaagc acccccttgt tgatacattt
    12241 attgaggaaa ccatcttatt tggattccag tatcctgaag tcaaaggttc tacggctgag
    12301 tggcaggtcc aggtaaaccc ggcccacatt ctgaatgtac ggtcttggct cggtattata
    12361 gcaatgaaac caagatggac taagcgactc ctctcagcgt tgattatcaa cctcaagctg
    12421 ggaggtattt ttgttcgcga cactgatctc attcagaagg atatttctgc tttgctgaac
    12481 tctgatatag gcccggcata caaccttaca aaacaacttt tgagaatttt tccagtctat
    12541 ttcagtgaaa taggggcgga aggtgaattg agagatatat cgaccaaggt tgatgaactc
    12601 tcattcagaa atgataaact tataaatttt ttgcgaaaac aatctcatgt tgagagcaac
    12661 agcctgcttg taggttttgt agagaatgct tttctttatt ggtattcagg tgataaggat
    12721 atccttaaaa aagacctgcc aaatgaagtg tatgatcaga ttgccaattc cggagagtat
    12781 tttgatgggc ttcactccat attcaaatat attatggtga aggcgaacaa cgatcctgca
    12841 atcttactca gttgggatag agccaaggca cacaaagaaa tttatggcat cagaggggtt
    12901 tctttaaggg ataaagagcg ggcactacta atgattaggt tttatcagct tctccataaa
    12961 aaatacaatc cccaacatac cgagctcttg aaagatcttg aatcaaatta tgtttttgat
    13021 cgttctcaag ttaaggcgtt acaacgatca ctgaagagaa aaaactttac caggagtctt
    13081 tcgattatac tcgactttct tggcttactg aaagagaaaa tactttcgcc gcaaaagact
    13141 gaatattttg agaacattta ccacaaaaga catattgcag caggtatccc ttcaatgtat
    13201 ggtacctatc atgaggaaaa attcgaagct gttggtcttt cccttcgtct ggaaagcctc
    13261 gctacgttcc tgtttgaaga attgatcagg tcgatgaacc tcaagtttat taccaagagc
    13321 acactgataa tgatacatga ttgccttggc ctctatatca gagcactcga attggaaggc
    13381 atagcaacag aaggtctcgt ggcaaaaatc aagtatgtca caagtgcatt gcaggtaaaa
    13441 atgttttctg tagaccaata tatagatata ttccgtttta tttcaaaggg catccagcac
    13501 attatccggg actactatgt cgatgcccat cgcctgaatc ttcccattat agtaaaacag
    13561 cttatagaag cccaaaatga tggccaacac caggcacagg aacaacgaca agaagagatt
    13621 atttaccagt attcagaaaa ttttgtccga ggaatgattt caactgcctt tggtctccag
    13681 gtactggaca atcttgtgaa cggtgttatt gagactttga atgcagagct tgaacgtttc
    13741 aaggacaaca aacagattct caaccttgtt atggcctata gccctgatct ggcaatttca
    13801 tcaatttacc agcgaaataa acaacttgac aaccagatat tgctcggcaa caaagggtac
    13861 tttctaaaag agctcgcttc ttttgggttc cgcgttccac cgggttttat cctgactact
    13921 gagatattta gaggatatga tgctgttgtg ggttataagt atattttcaa ggacctgaca
    13981 atcaggatta accgtgaaat tgctgaactt gagaagataa tgggatccag atttggagat
    14041 ccccgtaacc cgttgcttct ctctgtcagg agcggagcaa ccatatccct gcctgggatg
    14101 atgaattctt tcctcaacgt gggaattaac gaggatattg ctgagggact tggaaggagg
    14161 aggggacatc aatgggcagc ttgggattct tatcgtcgat ttctgcaaac ctgggggatg
    14221 tatcaaggtt tgaaaagaga tttttttgac gatatcatga atagttttaa aaccaagtac
    14281 gatgtgaaaa agaagataca gttcaagcct gatcagatga agcgaattgc acttgcctac
    14341 aaaggcagga tggcggaaaa ggggattaat gtggctgaca atccctttgt tcagttgcgg
    14401 gaagccattt tgcaggtttt tgcatcgtgg tactcggaac aggccaagat ataccgccat
    14461 cagatgcgtt tgtctgatga atgggggact gcggttatag ttcaggcaat ggttttcggc
    14521 aatcttaatg cgaactcagg gtctggagtc atcttcacaa gaaatccaaa gggtacctca
    14581 gctaatgtca gtttatctgg tgattttatt tttggagtac agggtgatga tattgtttct
    14641 ggtctggtca tcacttaccc aatctcagaa aaacagcgac tggcagaaaa aagagcattt
    14701 gaaatttcat tggagaataa attccccgag atttacaatg aactggtaag gctgtcagag
    14761 attttaatat atgaaaaagg atttaatcac caggagatag agtttacatt tgagaattca
    14821 acaccggata agctctatat cctgcaaaca cgagatatgg ttcagagaca aacaaataaa
    14881 gtaaagcgtt ttaaggatac agaggctctc cacaagtcgt atctcgggac tggtattggt
    14941 gtgagtggtg gtgccttgac cggaaaagcc gtctattcag aaaaggatat aaaacgtttt
    15001 cgaaaaagtg agcctgaaac tccactgatc cttattcgac ctgatacagt accagatgat
    15061 gttggaatac ttttgcaagt tgagggtcta ttgactgcac gtggaggtgg cacctcgcac
    15121 gctgcagtta caatccccca gctcgataag gtgggcgttg tcggctttaa taagctacag
    15181 gtgtatgaaa cggatggatg gagtctggtt gatggcctga agataaagag cggtgatttt
    15241 ataggaatag atggatggag cggcgctgtt tacctgggaa aaaatgagtc agagtctgag
    15301 gaatcatata atataaccct ctaagcacag cctggattct catgcgtcaa attttgaggc
    15361 acaaatgcct ataatttttt gtgattgaag gaagaagact gatttttgaa cagttttcat
    15421 ggtggtttgc ttggttttcg ggttgcaact tccaggatat taataccagg tatttgcaac
    15481 ccgaaaaatt atttaaagca aatttttatg cacgtggacc gttagatccc ccatcctgtt
    15541 tgtgtagctt ccgtattcat tgtcatacca tccaaaaatt ttagcgtgca caacaggtac
    15601 tcgcaatgtt tttgattcca taacctgtag agcctttgca gaaaagttag gtatctgggt
    15661 aaggtctaca ttgatgaatg cagtgcgtga atggttgaac tgaccctcaa taatcaccgc
    15721 agcgttaatg ccgataaggt cggtagagac attctgttca gttgtataga tgagaagatg
    15781 ctcggggttg ctctcgtacg tttttttgta aatatcgttg attgctttgg tattgatggc
    15841 tgctgaagtg ccatcagcat tcatctttgt ctgaaatgta acatttagga taatgagtga
    15901 aagcgtgttg gttggtatcc tgattgagtc tgccataaaa ccaatattct taacctctgg
    15961 gataacctgt cccagagcct ctgcagcatt ggttgaggtg agaataatgt tgtttaagat
    16021 actcctgttt tttctaagat ccttttcacc tgctttggga acactgtcaa gaacactttg
    16081 agagtttgta actgcgtgaa ccgtcgacat tgaggcagtt aaaatggtat tcgtcttctc
    16141 attttcgaga agcggtttta ccatgtgtgc gaggccggtt gtggtgcaag atgctgctga
    16201 gacaatctgg tgcttttttt gatcaaatgc agaatggtta attccataaa tcagggttac
    16261 acaatcagct ggcgtggaca atgctttatt ttttatctta aaggcagaag aatttattac
    16321 tgcttttgca ccttttacga ggtggcctct gatagaaccc ttggaatcgt ccggctgtac
    16381 tgtgggatct ttgaattttc ctgtacaatc tacaacgagg tcaacattgt aatcgcgcca
    16441 tggaatatcg accgggtttc tcgcttctcg aaggatggtc actgggatcc catcgataac
    16501 cagtttgccc ttcttttcat ctttgatttc tataagacgt tctgatttta ttccgtaaag
    16561 aaaccgatga agaaggccgt aggttgcatc cttctctatc atctgtgcaa tcgactgcag
    16621 gcctgtacca gattcccttc caagattaac gatgatttct ttaaaatact ttcgaccgac
    16681 gtgatgccac agtgttagct ttccaattct tccgagactg ttgattccaa gagttggtgg
    16741 ccgattttca aaaacctttg cccttgccat gatgtcttac ctccttgtct ccgtgaacgg
    16801 gagagttgta gtggatttgg gtatgtattg gttttgatac gatggagata ttctgtaaaa
    16861 cccgagcacg gcccatttga atctgataag ccatatcaat gtactcgtcg atgacactgt
    16921 tcatcatcct ttattaaatg tgaattttat cacaaaacat tataaaagtc aactgtttca
    16981 gtcatgcccg gttttgctgt attctatcca ttctcactgt tgtatggggt aaagttgtga
    17041 tataatttta tcattactgt gaaggggagg atgcaatggc gttcattaga gagccatcgg
    17101 taagtggcac attttaccct gataatccaa aaattctcaa gaaaaccata gaggattatc
    17161 tccaaaatgt gatttttgaa ccatcgaata aagaggtcat cggtcttcta tctccccatg
    17221 caggatatat atactccggt caagtagcag cctatggtta caaggcaatt atgggctccc
    17281 actatgatac agtgatcgtc atagctccca gccataggag ccattttgaa ggcagcgcaa
    17341 caattgtaaa gggagggtat cgcaccccac tgggggttgt tcaaatcgat gaagaactgg
    17401 ccggagagat agttagcctc aaaacggttg tttggcctga tatagatgcc catagggatg
    17461 aacattccct cgaagttcag gttccctttt tacaggtgat actgaaaaat tttaggttgt
    17521 tgcctctcat cttagggtct caggagccat cagtgtttgt ggcccttgtt gataccctgt
    17581 atcgatcaat caaaaaaacc aacagaaaag ttcttgttgt gggcagtact gatttgtcac
    17641 attattaccc atatgcagaa gccgtcaggt tagatacagt ggtgaagcaa cgtcttcaat
    17701 cctatgacat agaaggactc caggatgatt tttacaaagg gacatgtgaa gcatgtggaa
    17761 cgggccctat tttgaccacc atgatgcttt caaaaaaact tggagcttcc tatgctaagg
    17821 ttctgaaata cgcaaactca ggtgatgttt ccggagacag gagcggtgta gttgggtata
    17881 tttcatgcat tttcaccatc aacaaccagt gagaaacgga gaaattatat tgaacctttc
    17941 gaatgaagaa aagaagaagt tgaaagacct cgcacgggat gccatagaat gtgcattgtt
    18001 cggtaaggag caatgtgaaa tagaactatc ggaaaaatta aaagaaaagg caggtgcatt
    18061 tgtaacactt aaaagcaaag gtgagttgag agggtgtatc ggtcatatca gcgcttccat
    18121 gccgctttct gatgtgattg agaagatggc aatagagtca gcgtttcatg atcctcgatt
    18181 ctgtgctcta cgaaaggatg aatggaaaga tattgacata gagatatcgg ttatatcacc
    18241 tatgcgtcag attaaagata tagaagagat tgaagtcgga gttcatgggc tctacattgt
    18301 aaagggaggg tattctggcc ttttgctccc tcaggttgca acagaatatc attgggacag
    18361 aaaaacgttt ttggaataca cctgttacaa agcagggctt tcaaaagatt cgtggaaatc
    18421 aaatgatgtt aaaatctata tcttttctgc tgaggtgttt taatttactt gtttggcctt
    18481 tacagaaaag attaccctag aaaggagacg ggatgatcaa tgggagtatg ttgatagaag
    18541 atgccgtgag taagtacccg gaaaccatcc ctgtttttga gaagtttgga tttgggtgtt
    18601 tggggtgtca agcagctttg tttgagagca tagagcaggg tgcgaaaatc cacgggataa
    18661 atgtggaagc tctcattaca agcctcaata aagccatcgc gaaacgatgt tccataactt
    18721 gactctgaat tctgttttct gaccataccc acatatgaaa atgtttttag ctataatttt
    18781 ggctgctgga acttcaagca ggctgggctt caataagctg accgtgttta tagatgggga
    18841 atcagttatt cggagaagcg taacaccatt tctcatggct gaaattgaca ggattttggt
    18901 tgtgacggga aatgacagcg agaatgttaa agagcagctt gaaggattac cagtgagctt
    18961 catttacaac ccttgctaca agaaagggat gtccagttca attaaaacgg tgacaccatt
    19021 ccttgaaggc tcagaagcag ttctttttca tctcggagat aaaccttttg tctcatctca
    19081 cagcataaag actgtcctta atacctttag agccgggaag gctaagattg tcttaccgcg
    19141 ttttgacgga aaaacaggac accctgtcat gatcagcact aaactgctcg aagatgaaat
    19201 cggaaacctg catggagaca ggggactcag ggaagttata gaaaaataca agcaagatgt
    19261 ggtatttata gaaggtgata ctggaaactt gtttgacata gacactatcc aggtattgga
    19321 ccaactccgt gaaagaggtt acacaattga agaaaataag cgttgaaaaa gcacttggga
    19381 tgacacttgc tcatgatata acagaaatag tgccaggaaa gaaaaaagat gtggcgttta
    19441 gcaggggaaa aattattgaa aagggagata ttgagagact tcttgatctc ggtaaacgtt
    19501 atctctatgt ttttgaaggc cctgcaaagg gagttcatga agatgatgca agcaccagga
    19561 ttgcgcaggc catcttgggg gaatatatgg aatataccag ccctaaagaa ggaaaagtaa
    19621 cgatcaaaac taccgaaaat ggcctttttt atgttaacgc acagcatctt ttcgcaataa
    19681 acaagattaa gaatgtgctt ttgagtacac taccgaatcg tcatctcgtc aaaaagggtg
    19741 atattattgc agcaacacgt ataatacccc tttacattga tgaaaaatcg ctaaaacggg
    19801 ttgaaaagat aggggagggg cgtgttatca gtgtaagtcc gttcaaatcg ttaaacatag
    19861 gtctagtcat tacggggagt gaggtgtata ccggcagagt acaggacggc tcttcacttg
    19921 tagaaaataa attgaggaac tatggtttga cagtcgcggg aaagataatt gtccctgatg
    19981 agatttctat gataaaagat gctattctca gccttttcaa ttctggggta gatcttgttc
    20041 taactactgc aggtttatct gtggacccgg atgatgtcac aaaagaaggg attgaggcca
    20101 ctggagcaga aacccttttt tatggtaccc ctgttttccc cggtgcaatg tttcttgttg
    20161 cgcggctcag aggaaaatac attcttggtg caccagcctg tgtgtattat aacaggaata
    20221 cagtgcttga tataatcctt acaagactgc tggctggcga gagaatgcat aaaaatgata
    20281 tggtaaaact ctcctacggt ggtttgtgtt tgcagtgtga tgaatgtcac tatccgacat
    20341 gtttttttgg aaaggggtta tgatgaagaa cagttcgtct tttatagttc agcattcagc
    20401 gaaaaataaa aagagagaga aaaaaatcca cgacctcata gtggctatta aaggggctgg
    20461 agagatggca accggaattg cccacagact ctatgaggcc aacattagac gcattgtgat
    20521 gacggaaata cctgaaccga taaccgtcag gaggacagtt gctttttccg aggtagttta
    20581 tgagggtgag gtaattgttg agggtgttca tgcaaaacgt gcagtgaacg tcgatgaagt
    20641 aaacgatatt tggcagcatg atatgattgc tgttgttgtt gacccaaaat gcactaccgt
    20701 gagtgttctc aaacccgata ttgtcattga cgcaattatg gcaaagaaaa atttaggaac
    20761 aaaaaggacc gaagcccctt ttgttatagg tgtcggacct ggatttatag caccagatga
    20821 tgtacacgct gtggtggaaa gtaacagagg gcataacctt ggtagggttt tttatgatgg
    20881 tgcagcagaa cctcacacag gagtgccagg gccgaccatg ggctacacat cggaaagggt
    20941 gtta
//
LOCUS       DAOOMC010000038        20919 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdus sp. isolate AMR_MDS_2685
            NODE_2504_length_20919_cov_6.250815, whole genome shotgun sequence.
ACCESSION   DAOOMC010000038 DAOOMC010000000
VERSION     DAOOMC010000038.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864760
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdus sp. (wastewater metagenome)
  ORGANISM  Syntrophorhabdus sp.
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae; Syntrophorhabdus.
REFERENCE   1  (bases 1 to 20919)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20919)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,729
            Genes (coding)                    :: 2,708
            CDSs (with protein)               :: 2,708
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 7 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20919
                     /organism="Syntrophorhabdus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2504_length_20919_cov_6.250815"
                     /isolate="AMR_MDS_2685"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2699756"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..872
                     /locus_tag="PK125_05435"
     CDS             <1..872
                     /locus_tag="PK125_05435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB37582.1"
                     /translation="IFLLALILGFLWDTGAHYTQRLILPGLAVIMTISSLGISGDTFR
                     SAKTFIKSSLAGIIGNFFLHGLILIGLSMLVIKDQAFWNGFILLAAVPPAVAVIPFSL
                     FLRGNMELALFGTIGAYIGGLMITPLVALTFLGTGFISPMKLLLVFGELIIGPLLLSR
                     ILIWTGLLKKIDLYKGPITNWSFFLIIYIITGLNRDIFIQQPLSIVPVALVAILTTFV
                     LGWAIERAGSLLEFEKSTVTSFELLVTFKNYGLSGALALVFFNPQTAVPSTVTSAIGI
                     LYFIWLEMRINKK"
     gene            complement(1220..2503)
                     /locus_tag="PK125_05440"
     CDS             complement(1220..2503)
                     /locus_tag="PK125_05440"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HPB37583.1"
                     /translation="MPFEICSIRPPTENYSLTFRLTRNCGWNRCMFCPVYKTGALFSR
                     RSIEEVKRDINNAKQIDDILVDAGLFNNSFSAKSFLDIDKIILEIRKANPDCDSIPLS
                     PAKGKGAPTATTHEDEDERMAWFSSWFRETPAIEESVYHVATWRRYGGRSCFLGDANS
                     LILSADFFSEAVDYMRDRFPALSRFTIYGRTKSAAKKKLDEMKAFSRAGLDRIHFGIE
                     SGSDGVLSFMRKGETARDHIEGCRKTREAGISPSVYVMPGLGGAKWSIEHAIETARVL
                     TEAKPDYIRLRTLEIFPGTGLLQAMKAGEFKEATEDQIVREIRVIVEQTDCETEIVSD
                     SASNLLDISGRLPHDRARMLSVIDGYLGLSEREKIVFSLSSRLNSFVGQYGGISDDIG
                     EAIRPYVRDGAIDPSGASDEELKWVIRLIRSKLMP"
     gene            2975..4678
                     /locus_tag="PK125_05445"
     CDS             2975..4678
                     /locus_tag="PK125_05445"
                     /inference="COORDINATES: protein motif:HMM:NF013188.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HPB37584.1"
                     /translation="MDLDSFITSAAILLIVASLMITLFKHLGLGSIAGLLVAGIVVGP
                     HTPGPYITTHVEGVRSFAELGVVLLLFVIGLEIKPSRLWSLRREVFGLGSLQIIITAL
                     AITGFALWNHWHWKASLVMGLTMAMSSTALVMEMLHDKGEVASPQGSAAFGVLLMQDL
                     AVVPMLALIPMLAGPGAFSISIPGWKQLGIILGLFLLIFTLGKYVVPFALERLAHQRN
                     REGFVVVMMLSVFLSAWAMHRAGLSFALGAFLMGVFLSVSRYSLQIEAYIEPYKGILI
                     GLFFVAVGMSIDLRSIVASPLAFVKYAAAVICIKIIVMGTLCLIFGMNRSISTKISFL
                     LAQGGEFGFVLLSSAKVLSVIDNDTFVMGIEVISVSMLFTPLMAKLGNYLAGYFMPKK
                     SAEDIISIPDETGEIKRRVILGGYGRVGHVVAVLLHSSNVPFIAFDNDPARVAKGKGD
                     GFPVYYGDIANPALLAAAHAEQAALVVLTVAQEKITLKAISHMQNNYPSIPVIARAWD
                     LEASGKLIQAGATRAFPELMETSLRLATDALRMVGVPVDNVDLLVSDARRRDYELVNP
                     G"
     gene            5170..5607
                     /locus_tag="PK125_05450"
     CDS             5170..5607
                     /locus_tag="PK125_05450"
                     /inference="COORDINATES: protein motif:HMM:NF039818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB37585.1"
                     /translation="MKLPEYITKSEVQKICKEIEIRDWTKLKKAAVLPEEAKKILSLV
                     NVENMKIDLKEFTTGLEVELEHGVRFKDANVTNNHPIITGMIVLAHLKESLDYYKLLD
                     VAEAEGDLLKAVAAGNMAKTKKYYKKVAQAKIVLSQAVASQVK"
     gene            5632..7137
                     /gene="cobA"
                     /locus_tag="PK125_05455"
     CDS             5632..7137
                     /gene="cobA"
                     /locus_tag="PK125_05455"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: protein motif:HMM:NF004790.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004851 - uroporphyrin-III
                     C-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0019354 - siroheme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HPB37586.1"
                     /translation="MGKVYLVGAGPGDAKLITLRGLELIQKADVIIYDFLVNKELLAF
                     AKEDAEIIYAGKQASKHELPQKDINALIVNKAKKDVVVVRLKGGDPFIFGRGGEEAQA
                     LAEHGVSFEIVPGITSAISVPAYAGIPLTHRDFASTVAFITGHEDEKKTESTIRWNEL
                     ATGPDTLVFLMGVKNLRTIKERLIKGGRSPETQACLIQWGTLPKQKVVVGKLGEIDIV
                     AEKAGIQPPAIIIVGNVINLRRQLQWFESRPLMGKKIAITRAQHQSIRLGEKLTEAGA
                     EVLYYPTIDIRPIRPNRKLSHAIDIIDQYACIIFTSTNAVSVFFEALSAKTRDARALA
                     GVKILPIGQATASLLESKGIIPDFIPDTFTSEGIVETLKTIGINGKRLLLPRAEEARD
                     IIVRFVRDHGGTCDVIPIYRTALPKNIVPLTSKPDIITFTSSSTVKNFVTLYGKKTLG
                     KMVIASIGPVTTKTINSLGLTVHIEAERYDIPGLVEAILEYVKPVPVTHNR"
     gene            complement(7288..8340)
                     /locus_tag="PK125_05460"
     CDS             complement(7288..8340)
                     /locus_tag="PK125_05460"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPB37587.1"
                     /translation="MISIRNISANAGSFILKDINLEIKDGEYFVVVGPTGSGKTILLE
                     CLAGLQRLRGGEILISGKNITPVPPERRELGYVPQDYALFPFLDVRENIIFGLQPKKI
                     SKQELEKKVSSLANLLSIHHLLDRNVRTLSGGEKQRVAIARALAISPKVLLLDEPMGS
                     LDVRTAKYLRLELKRYHEELGITTIHVTHNLIEAEEMAERMAILHMGQIEQVGTPDEV
                     FFYPKSPFVADFIGTPNILDCDYYNDIGHGLIEAVCGGVPIILPHHDGPVRKLTLLPR
                     DVYVTTRTPPGPGINRFRGTVMEITPFSSLIKLTIKVGEINLLAELPQDIFEEMDIRT
                     GHEVHLILKLRSLRVY"
     gene            complement(8337..9143)
                     /locus_tag="PK125_05465"
     CDS             complement(8337..9143)
                     /locus_tag="PK125_05465"
                     /inference="COORDINATES: protein motif:HMM:TIGR01581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPB37588.1"
                     /translation="MKGFFSLPGERLFHVATLSALGFLLLFFVGIISSLATYTDWRQF
                     FSILFSKEILFAIRLTLFTSTMATIISMVFAVPAAYAISKTNFRGKNIVDTILDLPIV
                     ISPIALGAALLVFFNTSLGRALEGNLMRFVFEVPGIVLAQFTIISALAVRLLKATFDS
                     INPRYERVGRTLGYTKIKVFFKVTLPLARNGLIAASILTWARAVGEFGATVTVAGAMK
                     MKTETLPIAIFLNLAQAEVERAIAVVFVLVAIAVIALVILRRFTSRRQTS"
     gene            complement(9140..9313)
                     /locus_tag="PK125_05470"
     CDS             complement(9140..9313)
                     /locus_tag="PK125_05470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB37589.1"
                     /translation="MKSSASSRGFIGVLLSDEGTRVFGKWACGVIERGVERLIMNALI
                     EEECILTEGGNRK"
     gene            9618..10031
                     /locus_tag="PK125_05475"
     CDS             9618..10031
                     /locus_tag="PK125_05475"
                     /inference="COORDINATES: protein motif:HMM:NF012354.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HPB37590.1"
                     /translation="MILFHVNISDAGICRQISYREIFELKIAYKVWLDNDGKAFGEGP
                     YQLLKKIETTGSLHQAAIEMKMSYRKAWTTLKNIETRLGFPLLERKIGGIAGGGSQIT
                     KQGYNLMRHYEEFRTSLKEELQNLYQKHFGYLSNK"
     gene            10031..10831
                     /gene="fdhD"
                     /locus_tag="PK125_05480"
     CDS             10031..10831
                     /gene="fdhD"
                     /locus_tag="PK125_05480"
                     /inference="COORDINATES: protein motif:HMM:TIGR00129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HPB37591.1"
                     /translation="MSLVDIPIIKYENGKTTNIYDAVAQEEPLEILINDQPCHITMRL
                     PGEEIPLILGFLFTEGVIGSIEEVLTLSHCNDVSGNRMNVHLKTSTDKDLEKFISRKS
                     SSFTHCGTCGKDIVTNLSQSLSKIPKTVNITFPQLAEFQSKLIKGQQTFQHTGGTHAA
                     GIFDRYGTLLALSEDIGRHNALDKSIGKVLLARKADEAKILILSSRLSYELVSKAALL
                     NMEIVTGVSSATSLAVELAKNMEMTLIAFHRGQRGNICSCPERIALEE"
     gene            10940..11239
                     /locus_tag="PK125_05485"
     CDS             10940..11239
                     /locus_tag="PK125_05485"
                     /inference="COORDINATES: protein motif:HMM:NF018168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod C-terminal
                     domain-containing protein"
                     /protein_id="HPB37592.1"
                     /translation="MRKLENAARNIANSNTDSYKAKKSAAVEDGLGLPVLNITPDNAP
                     GTTFHDPDGETHESSNVDLSMETTDVLIAKRGYEANLKSVKAQEEIFDSLLDIIV"
     gene            11458..11745
                     /locus_tag="PK125_05490"
     CDS             11458..11745
                     /locus_tag="PK125_05490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB37593.1"
                     /translation="MKKIFLITLAVALVAVIALPSSGYCRGYYGGYRGHHHGNGWWIP
                     GAILGGLFLGSVLSSAASPPVYYAPPPPPPPPPRYYYYYYPPAPRVYVYPY"
     gene            11943..12281
                     /locus_tag="PK125_05495"
     CDS             11943..12281
                     /locus_tag="PK125_05495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB37594.1"
                     /translation="MDAYAVCDAMIFGKGVFGVFSTIEKALHFKSEAEKEGMHVCEIK
                     QLTITGPYEFPGNIFAAYIYDLLYDMYTLDGLYAKFEIASDAAGKRGLIDEFIIDIPG
                     KKTVIINDPS"
     gene            12306..12938
                     /locus_tag="PK125_05500"
     CDS             12306..12938
                     /locus_tag="PK125_05500"
                     /inference="COORDINATES: protein motif:HMM:NF019265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HPB37595.1"
                     /translation="MISLSKRESDRNKPQQNDDNHHVSMCQKGDVNAFQVLVERYQKK
                     MLNMAYRMTGDYEEACEITQEAFLSAFKAIRKFRGEASFATWLTRITLNQARNQLKKT
                     RYISHHEGVPIDAPVDTKANQVYDSPASQEETALEQLERKELQSEVQKCIDTLPDEFR
                     EVLVLRDIQEFSYDEIRDILGVPEGTIKSRLFRAREAMKNCLKRVVGDIR"
     gene            12935..13921
                     /locus_tag="PK125_05505"
     CDS             12935..13921
                     /locus_tag="PK125_05505"
                     /inference="COORDINATES: protein motif:HMM:NF021550.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2275 domain-containing protein"
                     /protein_id="HPB37596.1"
                     /translation="MTCKEIEEKIAAYQEGMLSPEERKLVDDHLVTCPKCSSALADLE
                     KAIAIIEGLPEIEPPPWFTQKIMAQVREEAPEKGSILKRLFYPFHVKIPIEALATLCV
                     VALGLYAYKTSGPEIKTYQTPPPATIESPSKDSTVDRQKEEIKDLRLSETKIDEENFD
                     RKEPHVKPPVQTEARRPSSGLVMEQKAEAPVATGVAPPAAAPLIAKEKDVSVSTDNER
                     KQEREKKVFQLAPGHRKAEVQIPASITITLSVENIFSASTDAENLLRESGFVVTRKEY
                     RDGIQTLTVHCPAQKLSVLLNRLQAIGYLKDPGITEETTDGNVQVHIRIISR"
     gene            14247..15857
                     /locus_tag="PK125_05510"
     CDS             14247..15857
                     /locus_tag="PK125_05510"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HPB37597.1"
                     /translation="MRKNNLICKGKPTGPDDNFRIAIEHSNDGVAIMQGNRHVYVNQK
                     FLDMFGYKSIEEVIGKTHDLTVHPDDYEMVTEYNRWRQKGNHQVPSKYEFKGIQKNGA
                     IIYIEASSAAITYNGEPASLAYLRDITERKRSEVMLMESEKRYRNLVENALVGVYQTN
                     LDGDVLYLNNTCLHMLGFATLEDAKSGGSPSFYKNREDRKLFLEFIKQNKKVSNYEVE
                     FVTHSGYPLHILLSATLESDTITGMMMDITEHKQKDLALQESEARYRLLAENVSDIIL
                     MMDMDLNFTYISPSIERLRGPEPGHVPLRSLRDALTASSYDVVMNVFMEEIAKEHEGQ
                     SDPFRFRVLELEYVRYDGTTRWVEVKASFLRNADGLPTNILCVVRDITHRKEAEKELK
                     MKSLRLEEVNTALKVLLEQREKDRAELEDNILLNVRKLILPYVESLKQRHLDEEQKIY
                     LDVLETNLQNIVSPFAKKLTLIQERLTPAEIRVADFIRDGKTVKEIAGAFGVSESAIN
                     VHRHHIRSKLGLTNRKINLRTYLLSLGK"
     gene            15990..16394
                     /locus_tag="PK125_05515"
     CDS             15990..16394
                     /locus_tag="PK125_05515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB37598.1"
                     /translation="MDLLFFTSPSDTGGKIIAETIQSQHPEKRVEICTHPESLLRKLL
                     NHCDFEAIAVLLPDNEERMIELYSMQNVLAKTPVMLVLPGKDKFMEAMGYRLRPLLLC
                     YREISTQLVASELGNIIKRIERVLTYQSKQNH"
     gene            16413..16685
                     /locus_tag="PK125_05520"
     CDS             16413..16685
                     /locus_tag="PK125_05520"
                     /inference="COORDINATES: protein motif:HMM:NF027875.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen-binding domain-containing protein"
                     /protein_id="HPB37599.1"
                     /translation="MTKKNQNKRVTLQYYAPEARQVYLAGSFNEWDSGARALKQQQDG
                     NWKAIMYLKPGVYEYRFIVDGKWQNDPLCSELCGNGFGGENNILRV"
     gene            16777..17670
                     /locus_tag="PK125_05525"
     CDS             16777..17670
                     /locus_tag="PK125_05525"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPB37600.1"
                     /translation="MSHNFQHNRVGGNGVNIFMTHTSGYARMVKQWVKGSAKAFNRDE
                     ITWPTDERFAHLYHATHNIALAFGHVLRLMSHHISALEHQQNLRTADREHIKIIRRHC
                     SAGVNLAYKLMLFSGSIPIKPEPMDLNDVVRNIDSVISPILKSSANIEFDLSDENLSV
                     MGDERLLKEVLASLILNANDATYRNGGTIAVATRRVKREYRIMDRDKRVFIGSCALLS
                     VVDSGPGIETSIQNQIFEPFFTTRQSGEKMGLGLSITQSIVRAHRGCLNILSRKGEGT
                     MVRIYLPLIPSHPEDGNTEAA"
     gene            complement(17822..18364)
                     /locus_tag="PK125_05530"
     CDS             complement(17822..18364)
                     /locus_tag="PK125_05530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB37601.1"
                     /translation="MSTKKSKEIHAISLLTRVPDGMYSLKKAMGIMYNWAFIMILCLI
                     PLLDGCSAWRTSCLVVSDAESGRVVARLPVNPREPIIIEFVNSIYLAPVWETLVYEPQ
                     EGLYIVMVESPSEDVFRYYGLEPDSTGKVMLHRKVGKVRLRSIDYSSHRIKAGNSTLK
                     LKEVVQGGESLLLDVKDCSQ"
     gene            18417..19385
                     /locus_tag="PK125_05535"
     CDS             18417..19385
                     /locus_tag="PK125_05535"
                     /inference="COORDINATES: protein motif:HMM:NF014586.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPB37602.1"
                     /translation="MGKIREIPLAALKLNEFIKNKVKEIAEEVKDGIAINALSGGVDS
                     SVVTLLGFKALGGQLKTYFIDTGLMRHQEPQYVVSIFKSLGVPVELINAKKQFFNALK
                     GIEDPEKKRNAVTHVFYKEVFGDLVKKSGAKYLLQGTNYTDVEETVAGIKRQHNILTQ
                     LGIDTKKAYGYHVIEPVIELRKTAIRKVGRALGLPKELYGRPPFPGPALSARVIGEVT
                     PQRVEIVRKATRIVEEELGKTSAFQYLAILHQDRVTGMRNGKRDFGLQIEVRCWDSKD
                     AREGTPTRLPFDTLLKLGDRITGEVPGVVSVTYNITRKPPSTIEAI"
     gene            complement(19474..19809)
                     /locus_tag="PK125_05540"
     CDS             complement(19474..19809)
                     /locus_tag="PK125_05540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB37603.1"
                     /translation="MKKILLSMVAVMVFVSFSFAAGPKTYQVTGPVLEIKDDIIVVQK
                     GKEKWEIAKDAATKVAGELKVGAKVTIHYTMKAVSVEVKDAGKKADAKKAEPAKTEAK
                     PAAPAPAKK"
     gene            complement(19875..20546)
                     /locus_tag="PK125_05545"
     CDS             complement(19875..20546)
                     /locus_tag="PK125_05545"
                     /inference="COORDINATES: protein motif:HMM:NF024245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HPB37604.1"
                     /translation="MHLLIFFAGIIGLVFFLGITEACAQVSKTLIDINTASEKELEEI
                     KGVGPATAKKIIAGRPYKSIDDLKKAKIPDKTIESMKPYVKVGAAPAAAPTAVETKPG
                     VPAKAVSDSKQATPAKPAAPPVAVKEAAKEAPVKAPAEKVKVSTKAHAREKARVPSKL
                     APGQKININTATKEELEALPDVGPVKAQAIIDGRPYKVPEDVMKVKGIKEGTFSKIKD
                     YIVVK"
BASE COUNT         6057 a         4718 c         4454 g         5690 t
ORIGIN      
        1 tgattttcct tctggcactc atccttgggt ttctctggga tacaggagca cattatacac
       61 aacggttgat cctccctggc cttgcagtta ttatgaccat ctcaagcttg ggcatcagtg
      121 gagatacttt tcgatcggca aaaaccttca tcaaatcgag ccttgcaggt attataggaa
      181 atttctttct ccatggactt attctcatcg gcctgagcat gcttgttatc aaggatcagg
      241 ccttttggaa tggatttatt cttcttgcgg cggtccctcc tgcagtggca gttatacctt
      301 tctccctttt tctccgtggg aatatggagc ttgccctgtt cggaaccatt ggggcataca
      361 taggtggcct catgatcaca cctctggttg cccttacttt tcttggcaca ggattcatta
      421 gcccgatgaa acttctcctc gtgtttggtg aattgattat cggtcccctt cttttgtcga
      481 gaattttgat atggaccggc cttttaaaaa agattgacct gtataagggc cccattacaa
      541 actggagttt ttttcttata atctacatta ttaccggcct caatcgagac atcttcatcc
      601 aacagcccct atcaatcgtt cctgttgcac ttgtagccat cctgaccacg tttgtcttgg
      661 ggtgggccat cgaaagggca ggcagcttgc ttgaatttga aaaatcaacg gtgacaagtt
      721 tcgagcttct cgtaaccttt aaaaattatg gcctttcagg ggcccttgcc cttgtcttct
      781 tcaacccgca gacagcagtt ccttcaacgg taacctcggc aataggtatc ctttatttta
      841 tctggcttga aatgcgcata aacaaaaagt aggctggagg gtttctccca ctgacacagc
      901 aggtactaca ctctatagcc ttcgatgggg atataactgt tcattcctct tttgtggtct
      961 tcagggatac cagctgcctg cagggtttca ataccttggc aaatactctt tttactcccc
     1021 tcaaggtctt tcatgagaga ccttgatgtc atcttttcat tgattctgtt tgttcagaat
     1081 tatcacagtg cattgaattt gtataacata atctctcttt ttcgtattaa acgcgaccag
     1141 attatactgc catacccatt gatggagtga atgagacaga agaggcagac aatgggaatc
     1201 tccaactccc agatgttttc tacggcatga gttttgaccg gataagccgg ataacccact
     1261 tcagttcttc atcagaagcc cctgatggat cgattgcacc atcccgtaca tatggtctga
     1321 ttgcttctcc aatgtcgtcg cttattccgc cgtattgccc gacaaaggaa ttcagcctgg
     1381 atgaaaggct aaaaacgatc ttctctctct ccgaaagacc aaggtaaccg tcaataacac
     1441 tcagcatacg tgcgcgatca tggggcaatc tcccgcttat gtcaagaagg ttcgatgcgc
     1501 tgtcactcac tatttcagtc tcgcaatcgg tttgttcaac aatgaccctt atctccctga
     1561 ctatctggtc ttctgttgcc tccttaaatt ctccagcttt catagcttgt aagagtccgg
     1621 taccgggaaa tatttcaagg gttcgaagcc tgatataatc aggttttgcc tctgtaagaa
     1681 ctctggctgt ctcaattgca tgctcaatag accattttgc accgccaaga ccgggcatga
     1741 cataaacaga aggagatatt ccggcctctc ttgtctttct gcatccttca atgtggtctc
     1801 tcgcagtttc gcccttcctc ataaaggaaa gtaccccatc acttccgctt tcaataccga
     1861 aatgaattct gtccaatcct gccctggaaa atgccttcat ttcgtcaagt ttctttttgg
     1921 ctgcagattt tgtccttccg taaatagtaa aacggctcaa agcaggaaat cgatccctca
     1981 tgtaatctac agcctctgag aagaaatctg ccgacaggat caaggagttc gcatcaccaa
     2041 ggaagcacga tcggcctcca tagcgtcgcc aggtagccac atggtagaca ctttcctcaa
     2101 tggcaggtgt ttctctgaac catgaagaaa accaggccat tctttcatct tcatcctcat
     2161 gcgtggttgc tgtgggagca cccttgcctt tcgcaggcga taatggtatt gagtcacaat
     2221 cagggttggc ctttcgtatc tctaaaatta ttttatcgat atccaggaag gattttgcag
     2281 aaaaagaatt attgaataac ccggcatcga ccaagatatc atcaatctgt tttgcattat
     2341 taatatcacg cttaacttcc tcaatactcc tgcgtgaaaa aagggcacct gttttataaa
     2401 caggacagaa catgcacctg ttccatccac agtttctggt taatcgaaaa gtcaggctgt
     2461 agttctcagt agggggtcgt atcgaacata tttcgaaggg cataacatgg gaaagattca
     2521 tttgccttac catttactaa ttctattctt cgattatact gcaaagctag cacagcttca
     2581 atgttatcgg tatattgtgt gaagataaat ctttgaacat agtgcagtgt ttttctgtaa
     2641 caaaacatta tatcgcaatg aaaagccctg ttcaaatggg tagcaaacat ggtaaactga
     2701 aaatctagaa caaattcatg aattctttag gtgtccaggt tttcttttca aagtggtggc
     2761 tgaaaacgat attttgctgt aagaatcacc cctattttca tgtccaccat ggtctttcac
     2821 agttatatct tctcaactta cctgagagaa cctatgggca acccaggaac actcctgatt
     2881 catgttctat caccgattac ttttcttgac gaagtttata ttcattctgt attattagtg
     2941 agggactctc gcaacatggg gcatcttcaa atccatggat cttgatagtt ttattacatc
     3001 tgccgctatc cttctgattg tcgcttccct gatgattaca cttttcaagc accttggtct
     3061 tggatcgatc gcgggacttt tggttgctgg aatcgttgtt ggtccccaca cacccggccc
     3121 atacataaca acccacgtag aaggggtcag aagctttgca gagcttgggg ttgtgcttct
     3181 tctctttgta atcggacttg aaatcaaacc ttcgcgtttg tggtctctga ggcgggaggt
     3241 tttcggcctg ggctccctgc agatcattat tacagcattg gcaattactg gttttgctct
     3301 gtggaaccat tggcactgga aagcatcgct cgttatgggc ctaaccatgg ccatgtcttc
     3361 tactgctctc gtcatggaga tgcttcatga caaaggcgag gtagcaagcc ctcagggtag
     3421 tgctgctttt ggggtcctcc tcatgcaaga ccttgctgta gtccccatgc tggccctgat
     3481 tcccatgtta gcaggcccag gcgcattttc tataagcata cccggatgga agcaacttgg
     3541 aataatcttg ggcctttttc tcctcatatt tacactcggt aaatacgttg tgccttttgc
     3601 actcgaaaga ttagctcacc agagaaaccg tgaaggtttt gtagtggtga tgatgctctc
     3661 agtttttctg tctgcgtggg ccatgcatcg tgccggtctt tcttttgctc tcggggcatt
     3721 cctaatgggt gttttcctgt ctgtttctcg ctatagcctg cagattgagg catatataga
     3781 accatacaaa gggattctta tcggcctctt tttcgtggct gttggtatgt ccattgacct
     3841 gcgctcaatt gttgccagcc ccctggcatt tgtcaaatat gctgcagcag tcatatgcat
     3901 aaagattatt gtaatgggta cactctgcct catttttggg atgaatagaa gtatttccac
     3961 aaagatttcc tttcttcttg cccagggagg agaattcggg tttgtccttc ttagttcagc
     4021 aaaagtgctt tcggttattg ataacgatac ttttgtaatg ggaattgaag taatatcggt
     4081 cagtatgctc ttcacacctc taatggccaa actcggtaat tatttggcgg ggtatttcat
     4141 gccaaagaaa agtgctgaag acatcatatc cataccggat gaaaccggcg agattaaaag
     4201 aagggtcatc ctcggcggtt atggaagagt aggccatgtg gtggcagttc tccttcattc
     4261 ctccaatgtt ccttttattg cgtttgacaa tgacccagcg agggttgcaa aaggaaaagg
     4321 agatggtttt ccggtttact atggagacat cgccaatcct gcattactgg cagctgccca
     4381 tgcagagcaa gcagcacttg ttgtcttaac agtggcccag gaaaagatta ccctgaaagc
     4441 catctcccac atgcagaata actatcccag catacccgtg atcgcgagag cctgggatct
     4501 tgaggcaagc ggcaagctta ttcaagcagg tgcaactcgg gcattcccgg aattgatgga
     4561 aactagcctt cgtctcgcta ctgacgcact cagaatggtt ggggtaccag tggataatgt
     4621 tgatcttctg gtttcggacg cccgcaggcg tgactatgaa ctggttaacc caggatagaa
     4681 gatcacagtg ctatcctgtt tcgatggcga tccctaaatc agggagacaa gctctttata
     4741 tagatactcc tgcctgattc aatacgaggt ttcgtcaaca aaactgaaga tagaaactgc
     4801 tgaagcgtac aacctgcaac accggccctt ttaaagaaac ctccagaact atatcccaga
     4861 atcttctgca gaattgtgac aatggggaca tgtcccaata tcttttcacc cctgtccgaa
     4921 acctctttat ctgatacaca aaccgaatgc cataactaga aatgatgggc aaacgattct
     4981 cggcaacctt acccggtaaa aggacaatct tgatcagaaa tcgagtagca tcagcagaaa
     5041 ttaggacagt gagccattat cgcataattc cttcacaatc ttttcttgta gagttattgg
     5101 agttctgtta gaattacagc caaataacag ggggttataa aatattaaaa gttaaagaaa
     5161 ggggtcacca tgaaattgcc tgaatacatc accaagtcag aagtacagaa gatatgtaaa
     5221 gaaatcgaaa taagagattg gacaaagctt aaaaaggcgg cagtactgcc tgaagaggca
     5281 aagaagattc tgtcactggt taacgtggag aacatgaaga ttgacctgaa agaatttaca
     5341 accggccttg aagtagaact cgaacacggc gttcggttca aagacgcaaa cgttacgaac
     5401 aaccatccca ttattacggg catgattgtt ctggcacacc tcaaagagtc ccttgactat
     5461 tacaagctgc tggacgttgc ggaagcagag ggggatctgc tgaaagccgt ggcagcaggc
     5521 aatatggcta aaaccaaaaa gtattacaaa aaggtggctc aggcaaaaat agtactcagc
     5581 caggctgtcg caagccaggt aaagtaaacg ccggcaactg ggagcattac catgggcaag
     5641 gtatacctcg tcggtgcagg accgggcgac gccaaactta tcacactccg tggccttgaa
     5701 ctgatccaga aggcagatgt catcatctat gattttctcg taaacaaaga acttcttgcc
     5761 ttcgccaaag aggatgcaga aataatttac gcgggtaagc aagcatcaaa gcacgaacta
     5821 cctcagaagg atatcaatgc ccttatcgta aacaaggcaa aaaaagatgt tgtcgtggtg
     5881 aggctgaaag gtggtgatcc cttcattttt ggccgtggcg gggaagaggc gcaggcactg
     5941 gcagaacatg gtgtcagttt tgagattgta cccgggataa catcggctat ttcagttcca
     6001 gcctatgccg gtatcccatt gacacacagg gattttgcat ctactgttgc ttttataacc
     6061 ggccacgaag atgagaaaaa gacagaatca accatccggt ggaatgaact tgccacaggg
     6121 cctgacactc tggtctttct catgggtgtt aaaaacttaa gaactatcaa agaaaggctg
     6181 atcaaaggtg gaagaagccc ggaaacccag gcatgtctta tccagtgggg tacgcttccc
     6241 aaacagaaag ttgtggttgg taagctcgga gaaatcgata tagttgcgga gaaagccggg
     6301 atacagcccc cggcaataat tattgtgggt aatgtaatca acctccgcag acagcttcaa
     6361 tggtttgaga gcagaccgct catgggcaaa aagattgcta ttaccagggc acagcaccaa
     6421 tcaatccgat tgggtgagaa acttaccgaa gccggcgccg aggtcctgta ttaccctacc
     6481 attgatatca gacctataag gcccaacagg aagctatctc atgcaataga tattatcgat
     6541 caatatgcct gtattatttt cacgagcacc aatgctgtat cggtattttt tgaggccctt
     6601 tccgccaaga ccagggacgc aagggcgctg gccggagtaa aaattctgcc cattggtcag
     6661 gcaacagctt cgcttcttga atcaaagggc atcattcccg attttatacc cgatactttt
     6721 acatccgaag gaatagttga gacattaaaa accattggaa taaatggcaa gagacttctt
     6781 ttgccccgcg cagaagaggc ccgtgatatc attgtcaggt tcgtgaggga tcacggggga
     6841 acatgtgacg tcataccaat ctaccgcaca gcattaccga aaaacattgt cccactaacg
     6901 tcaaaacctg acataataac atttaccagt tcgtcaaccg taaaaaactt tgttactctt
     6961 tatgggaaaa aaaccctcgg aaaaatggta atagcttcaa ttggacccgt aaccactaaa
     7021 accatcaata gccttggcct tacagttcac atcgaagcag aacgctacga cattccaggc
     7081 ctcgtggaag caatattaga atacgttaaa cccgtgcccg tgacacacaa tcggtaacac
     7141 gctgcatttc ttactggaat gggttcccct tcttgctttt gactttttgt tgctttttta
     7201 tgcccttcca gtttttctca tttttaattc cgcacctgct ttgcccggtg ccctatccca
     7261 ttgcccatgc cctctaacct gccttgttta gtatactctc aatgacctga gtttcagtat
     7321 cagatggacc tcatgtccgg tcctgatatc catctcctca aagatgtcct ggggcaactc
     7381 tgcaaggaga ttgatctctc caacctttat ggtaagcttg atcaatgaag aaaagggtgt
     7441 aatctccatc actgtacccc taaaccggtt aatgcccgga cctgggggcg tccgcgtagt
     7501 cacatataca tccctcggca aaagtgtaag tttccttacc ggcccgtcgt gatgggggag
     7561 aattatgggt acacctccgc acactgcttc tatgaggcca tggccaatat cattgtaata
     7621 atcacaatca agtatattcg gagtaccgat aaaatcagcc acaaatggac tcttggggta
     7681 gaagaaaact tcatcaggcg tcccaacctg ctctatctgc cccatgtgaa gaatagccat
     7741 tctctctgcc atctcctctg cttcaataag attatgggta acatggatag ttgtgatgcc
     7801 cagttcttca tggtatctct tcaattcaag tctcaaatat ttggcagttc tcacatccag
     7861 acttcccatt ggctcatcaa gaagcagaac cttgggtgag atggcgaggg cccgcgcaat
     7921 tgcaacacgc tgtttttccc caccactcag cgtgcgaaca tttctatcga gaaggtggtg
     7981 gatactgaga agatttgcca gactgctcac ctttttttcc aactcctgct ttgagatttt
     8041 tttgggctgt aatccaaaaa taatgttttc cctgacatcc aaaaatggaa aaagggcgta
     8101 gtcttgcggg acatagccaa gttcacgcct ttctggtggc acaggtgtta tgtttttgcc
     8161 agatatcagg atttctcctc cacgcaatcg ctgaagaccg gcaaggcatt cgaggagaat
     8221 agtttttccg gaaccagttg gacccactac aacaaaatac tccccatcct tgatctcaag
     8281 gttgatatct ttcaggatga agctaccggc attggcagaa atattcctga tggaaatcat
     8341 gatgtttgcc ttctgcttgt aaatcgccga agtattacaa gagcaatgac tgcaatggct
     8401 accaagacaa aaaccactgc tatagccctt tccacttcag cctgcgcgag gttgagaaag
     8461 atagcgatgg gcaatgtttc ggttttcatc ttcatggccc cagcaacagt cactgttgcc
     8521 ccaaattcac caaccgctcg cgcccaggta agaatagacg ctgctatcaa cccgtttctt
     8581 gcaaggggga gggtcacctt gaagaatact tttatctttg tataacccag ggtcctgcct
     8641 acacgttcat atcgaggatt gatgctgtca aaggttgctt ttaacagtct tacagccagg
     8701 gcactgatga tagtaaattg ggcaagcact atgcccggaa cctcaaaaac aaagcgcatg
     8761 aggtttcctt caagtgccct gcccagtgat gtattaaaaa agacaagcaa tgctgccccg
     8821 agggctatgg gagatatcac gattgggaga tcaagaatcg tatccacaat attttttccg
     8881 cgaaaatttg tttttgaaat tgcatatgca gctggcacag caaagaccat tgatataata
     8941 gtcgccatgg ttgatgtaaa aagtgtcagc cgtattgcaa aaagaatctc ctttgaaaaa
     9001 agaatagaaa aaaattgtct ccaatcagtg tatgtggcaa gagatgatat gattccaaca
     9061 aaaaataaca gaagaaatcc aagggcgctg agggttgcca catgaaataa tcgctctcct
     9121 ggcaacgaaa aaaatccttt cattttctgt tcccaccctc tgttagaatg cattcttcct
     9181 ctatgagagc gttcatgatt aaccgttcca ctccccgttc aataactcca caggcccatt
     9241 ttccaaaaac ccgtgttcct tcatcggata gcaaaacccc aatgaatccc cgagatgaag
     9301 cactgctttt catgtatacg aacattttgc cgggcgtaaa ggtaacaccc cagattctcc
     9361 aataacgtta aaaacggttt ttactctgct gttgcgactt tttgaaaaac ctttattttc
     9421 tttcatctgt tggctaaaag atctcttata aaaataacat tttcaagatt actgcataac
     9481 caaaacactc tcatataatc gacccctgat cagcacagcc taatctgctt caattttctt
     9541 tattcgttat ataccatagg gaatacccct gtcaactaat tgcgctacct cctgcgaaag
     9601 agaaatattt tatcgatatg attctgtttc atgttaatat ttcagatgct ggaatctgca
     9661 gacagatttc atatcgggag atatttgaat tgaagatagc ttacaaagtc tggcttgaca
     9721 atgatggaaa agcatttggc gagggtccat atcagcttct caagaaaatt gaaacaaccg
     9781 gttctcttca ccaagcagca atagaaatga aaatgtccta ccgcaaggcg tggaccacac
     9841 tgaaaaatat tgaaacaaga cttggttttc ctctccttga acgaaagatt gggggaattg
     9901 ccggcggggg atcgcaaata acaaaacaag gttataattt gatgagacat tacgaagaat
     9961 tccgcacctc actcaaagaa gaacttcaaa atctttatca aaaacacttt ggctacttga
    10021 gtaataagta atgagcctgg ttgatatacc aataataaaa tacgagaacg gcaaaacaac
    10081 gaacatctat gacgctgtcg ctcaagaaga accattggaa atattgataa atgatcagcc
    10141 ttgccacatc accatgcgtc ttcccggtga agaaatcccc cttatccttg gctttctgtt
    10201 caccgagggg gtgataggtt ctatcgagga ggttctgact ctcagtcact gtaatgatgt
    10261 atcgggtaac aggatgaatg tacatctgaa aacttctaca gacaaagacc ttgaaaaatt
    10321 tatttccaga aaatcatctt cttttacaca ttgtggaacc tgtggcaaag atatagtaac
    10381 aaacctttca caatcccttt caaaaattcc taagacagtg aacataactt ttccacagct
    10441 cgcagaattc cagagtaagt tgataaaggg ccagcagacc tttcaacata caggcggaac
    10501 acatgctgct ggtatttttg acagatacgg tacactcctc gccctttctg aggatattgg
    10561 ccggcacaat gcccttgaca aatcaatcgg caaggttctt ttggccagaa aagcagatga
    10621 agctaagatt cttattttaa gctcacgttt aagctatgaa ctggtatcaa aagctgcgct
    10681 gcttaacatg gagatcgtta caggagtgtc ttcagccacc tcccttgcag tagagctggc
    10741 aaaaaacatg gaaatgaccc tcatcgcttt ccacagggga caaagaggaa atatttgttc
    10801 ctgtcccgaa cgtatcgctc tcgaagaata aattataaac tcatacgtga tattaatcac
    10861 ttaggccgtt aagaggcaaa ccgataatgt aaatatgatc aatggattga gtgcatcagt
    10921 atctggcctt tggatgtttg tgaggaaact ggaaaatgct gcccgtaata tagccaacag
    10981 caatacagat tcatataagg ccaaaaagtc agctgcagta gaggatggtt taggtctacc
    11041 agtactgaac ataactcctg ataacgcccc cggaacaacc tttcatgacc cggatggtga
    11101 aacccatgaa agttcaaacg ttgacctttc aatggaaaca acagatgtgt taattgctaa
    11161 aagaggttat gaggcaaacc tcaagtcagt caaagcacag gaagagatat tcgattccct
    11221 tcttgatatc attgtgtagc caattttcca gtattgtgtt tcgacacatt tgtcgtaaaa
    11281 gaatcctttc ttcgacattt ttcacctatt tttgtgctcc ttaatataac aaatagtcaa
    11341 tattttcact aaattgcatt atatggaata atggcatcca gcttgctatc aaaagaatac
    11401 ggacatacaa aatatgaagt ccctgaatca tagaaaaagt agcacaggag gttgaacatg
    11461 aaaaaaattt tcctaattac attggcggtt gcacttgttg cagttattgc gttaccatcg
    11521 agcggttatt gccgggggta ttacggtggt taccgtggtc atcatcatgg aaatggctgg
    11581 tggattcccg gggccatttt aggcggttta tttcttggtt ctgttctcag cagtgctgcc
    11641 tctcctcctg tatactatgc tcctccgcca ccacctcctc ctccgccacg atattactac
    11701 tattattatc cacctgcacc gagggtttat gtgtatcctt attaataaca atagcaatgc
    11761 taaggattgg acataagtat aacaggatgg agagtttaga gttcaattca tagactgagc
    11821 aatgcatcaa cttgctttca ggcctataca tattgattgt ataggctttt tctattgaaa
    11881 tcgttcctac ttttattgtc tatacattgt atatttgatt catggaaaac aggagcaggt
    11941 caatggatgc ttatgccgta tgtgatgcaa tgattttcgg aaaaggtgtt ttcggtgttt
    12001 tttcaacaat tgaaaaggca ctccacttta agagtgaggc ggaaaaggaa ggcatgcacg
    12061 tgtgtgagat aaaacagctc acaataacag ggccttatga gtttccgggt aatatttttg
    12121 cagcatacat ctatgatctg ctctacgaca tgtataccct ggatggacta tatgccaagt
    12181 ttgaaatagc atctgatgca gctggaaaac gaggcctaat tgatgaattc ataatcgata
    12241 tcccgggcaa gaaaacggtt ataatcaacg acccatcttg aaaaaagcgc ccgggaacaa
    12301 aatacatgat cagcctgtct aaaagagaga gcgacagaaa caagccccaa caaaacgatg
    12361 ataatcacca cgtgtccatg tgtcaaaaag gcgatgttaa tgcatttcag gtactggtgg
    12421 aaaggtatca gaaaaagatg ctaaatatgg catacagaat gacaggggac tatgaagaag
    12481 catgcgagat aactcaagag gcgtttctct cagcatttaa agcaataagg aaattcaggg
    12541 gcgaagcatc atttgccaca tggttaacgc gaatcacttt aaaccaggca agaaaccagc
    12601 tcaaaaagac aagatacata tcccatcatg aaggagtacc tattgatgcc ccggtggata
    12661 ctaaagccaa tcaggtatat gacagtccag cctctcagga agagactgcc ctcgaacaac
    12721 tcgaaagaaa agaattacag tcagaagttc agaagtgcat cgataccctg cctgatgaat
    12781 tcagggaagt gcttgtacta agggatattc aggagttttc ttacgatgag atccgtgata
    12841 tcttgggcgt acctgaaggt acaataaaat ctcgtttgtt cagggcaaga gaagccatga
    12901 agaactgcct caagcgcgtg gttggtgata taagatgaca tgcaaagaga ttgaagaaaa
    12961 aatcgcagct taccaggaag gaatgctatc tcctgaagag agaaagcttg ttgatgatca
    13021 tcttgtcaca tgtccaaaat gttcatctgc tcttgctgac ttggagaaag cgattgccat
    13081 tattgagggt ctcccggaaa ttgaaccacc cccatggttc acccagaaga tcatggcgca
    13141 agttcgcgaa gaggcaccgg aaaaaggcag tatactgaag aggctctttt atccctttca
    13201 tgtcaagatt cccatcgagg cattagcgac cctttgtgtc gtcgctctgg gcctctatgc
    13261 gtacaaaacc tccggtccag agataaaaac atatcaaaca ccacctccgg caacgattga
    13321 atctccttcc aaagattcaa ctgtggacag gcaaaaagaa gagataaaag acctgcggct
    13381 ttctgagaca aaaatcgatg aagagaattt tgacagaaaa gaaccgcatg ttaaaccacc
    13441 tgtccagact gaagcccgaa ggccttcttc tgggcttgtt atggagcaaa aagctgaggc
    13501 tccggttgca actggagttg cacctcctgc cgctgccccg ttaattgcaa aagagaaaga
    13561 tgtcagcgtg tcaactgata acgaaagaaa acaagagcga gaaaaaaagg tttttcaact
    13621 cgcacctgga caccgtaaag ctgaggttca aatcccagcc agcatcacaa taactctttc
    13681 agtagaaaat atcttttcag cgtcaacaga cgctgaaaat ctactacgtg aatccgggtt
    13741 tgtcgtaaca aggaaggaat atcgagatgg cattcaaacc ctgacagtac attgcccggc
    13801 ccaaaagtta tcagtgcttc tcaatcgcct gcaagccata ggttatctga aggaccctgg
    13861 aataacagaa gagacaacag atggcaacgt acaggttcac attcgcatca tatccaggta
    13921 accgtttccc gttattgtat ggaaaaagat tggctcagaa tattccatta gattgaccag
    13981 tcgaaaacct gaaacgatac gctgatggca ctatttggat ttatatcata aattattcag
    14041 ctttaattgc agaaccattc aattttatat aactgaagcg gattgcttct gcccatggct
    14101 gggaaaaaga tgcatcagcg cttttatgca ctggtaaaca aaactgtttt aaacactttt
    14161 ttagccgaac ctggccggga aatggtcctg gagttctcca taggaatcat gtccgaaaat
    14221 agtgtatata taattgagcc atttccatga gaaaaaataa ccttatctgc aaaggcaaac
    14281 caacaggacc cgatgacaac ttcaggatag ccattgagca ttccaatgat ggcgtagcca
    14341 taatgcaggg taaccggcat gtatatgtca atcagaagtt tctggatatg ttcggttaca
    14401 aaagtataga agaggtcatc gggaagacac atgatctcac agtgcaccct gatgactatg
    14461 aaatggtaac tgaatataac cgatggcgac agaagggaaa tcatcaagtt ccttcgaaat
    14521 acgaattcaa aggaatccaa aagaatgggg ccataattta catcgaagcc tcttccgcag
    14581 ccattaccta taatggagag ccagcttctc ttgcatattt aagagatatt acagagcgca
    14641 agagaagcga agtcatgtta atggagagtg aaaaacgata cagaaacctc gttgaaaatg
    14701 cccttgtcgg tgtctaccag accaatcttg acggtgatgt cctgtacctc aataatacat
    14761 gcttacacat gttaggcttt gcaaccctgg aagacgcaaa atcagggggt tctccatcgt
    14821 tttataaaaa cagagaagac agaaagcttt ttttggaatt catcaagcaa aacaaaaagg
    14881 tcagcaacta tgaagtggaa ttcgtcacac attcaggtta tccccttcat attctcttga
    14941 gtgcaaccct ggaatcagac accataacag ggatgatgat ggatatcacc gagcacaaac
    15001 aaaaagatct ggccttgcag gagagtgagg ctcgctatcg ccttctcgct gaaaatgtat
    15061 ctgacattat cctgatgatg gacatggatc tcaattttac ctatatcagt ccatcgattg
    15121 aacgcctgcg tggccctgaa ccaggccatg ttcctttgcg gtccctcagg gatgcgttaa
    15181 cagcctcctc ctatgatgtt gttatgaacg ttttcatgga agagattgca aaagagcatg
    15241 agggacagag cgatcctttc aggttcaggg tactcgaatt ggaatatgtc cgatatgacg
    15301 gcacaacacg atgggtcgaa gtcaaagcct ccttcctccg taacgctgac ggtcttccta
    15361 caaatattct ctgtgtcgtg cgggatatca cccacagaaa ggaagctgag aaggaactca
    15421 aaatgaaatc tcttcgcctt gaagaagtca acactgccct caaagtcctg ttggaacaac
    15481 gtgagaaaga cagggctgag cttgaggaca atatcctgct caatgttaga aaattgatcc
    15541 tgccctatgt tgaatcctta aaacaacggc accttgatga agagcaaaag atttatctgg
    15601 atgttctcga aacaaacctg caaaacattg tctccccctt tgcaaaaaaa ttgactttga
    15661 tccaagagag acttaccccg gccgaaattc gggtagctga tttcataaga gatggcaaaa
    15721 ctgtaaaaga aattgcaggc gcctttggtg tatcagaaag cgcaatcaat gttcatagac
    15781 atcacattcg cagtaagctt ggattgacca accggaaaat aaatttaaga acttatctgc
    15841 tgtctcttgg aaaataataa taatttatta ttattttatt aataatcatt taacatcata
    15901 cctggttgat cttgtgctat cattatagtc catactacct ggttgcagga tttttttact
    15961 ccacattaca caaaggaagg ggaaaggctg tggatcttct attttttacc tcaccctctg
    16021 atacaggcgg caaaataatc gcagaaacca tacaatctca gcaccctgaa aaaagagtgg
    16081 aaatctgcac gcatcccgaa agcctccttc gcaaactttt aaatcactgt gattttgagg
    16141 cgatagccgt gcttctgcct gacaatgaag agaggatgat agaactatat tccatgcaga
    16201 atgtgctggc taaaacacct gttatgcttg ttcttccggg aaaggataag tttatggagg
    16261 ccatgggata tcgattgaga cccctgctcc tgtgttatcg agaaatcagt acccagctgg
    16321 ttgcttccga actggggaat atcataaaaa ggatcgaaag ggttctgaca tatcaatcca
    16381 aacaaaatca ttagaaataa ggaggctttt caatgaccaa gaaaaatcaa aacaaacgcg
    16441 ttacccttca gtactacgct ccagaagcca gacaagtata tcttgcaggt agtttcaacg
    16501 agtgggattc aggtgcacgg gcgctcaagc agcaacaaga tggcaactgg aaggccatca
    16561 tgtacctgaa accgggcgta tacgaatacc gtttcatcgt agatggcaaa tggcagaatg
    16621 accccctgtg cagtgaactg tgtggaaatg gatttggagg tgaaaacaac attctgaggg
    16681 tctaacgtac ttgttcgata gcctcacaaa ccaccagaag cagcacagaa agttgcatac
    16741 cagccaatga gctgttgttg aataaaggag gacagagtgt ctcataattt tcaacataac
    16801 cgagttggag ggaatggtgt aaatatcttc atgacacata ccagtggata tgcccggatg
    16861 gttaaacagt gggtaaaggg ttcagcaaaa gcattcaaca gggatgaaat cacatggcct
    16921 actgatgaga ggttcgcaca cctataccat gccacacata acattgccct tgcctttggc
    16981 catgttctca ggttaatgtc ccaccatatc agcgcgttgg aacaccaaca aaaccttcgc
    17041 acagcggatc gggagcatat taagattatt cgtcgtcatt gttcggctgg ggtaaacctc
    17101 gcgtacaagc tcatgttgtt cagtggcagc atccctataa aacctgaacc gatggatctt
    17161 aatgatgtgg taagaaatat tgattccgta atttccccaa tattaaagag cagtgcgaac
    17221 atcgagtttg acctctcaga cgagaatctt tctgtgatgg gggacgaaag gcttctcaaa
    17281 gaggttcttg caagcctgat attaaatgcc aatgatgcta cataccgtaa tggagggaca
    17341 atcgccgtgg caacaagaag ggtcaagcgg gaatatcgca taatggatcg ggataaaaga
    17401 gtgtttatag gatcctgcgc cctcctgagc gttgtcgact caggacctgg aatagaaaca
    17461 tcaatacaaa atcagatatt tgagcctttt tttacaacca gacaatcagg tgaaaaaatg
    17521 ggcctcggcc tttccataac ccagtcaatt gtcagggcac accgtggttg tctcaatatc
    17581 ttatcaagga agggggaagg aacaatggtg cggatatacc tgcctctcat accctctcat
    17641 ccagaagatg gaaacacaga agctgcttga tacagcgccg ttgtcttaaa agaaggtata
    17701 attattcatc cggcaatcat atatctgaat gttcccacct ctcatagagc aaccccacgg
    17761 ctgtagagat atttttccat cacgatggca tcgatgttca catccttagc atgttttatt
    17821 gttattgtga acaatcttta acatcaagaa gaagtgactc gccaccctga acaacctctt
    17881 tgagcttaag ggtgctattc cctgccttga tacggtgact gctataatca atgcttctca
    17941 gcctcacttt tcccactttt cgatgcaaca tcaccttgcc tgtagaatca ggctcaagac
    18001 cataataccg aaaaacatct tcagaagggc tttctaccat tactatataa agaccttcct
    18061 gcggctcata cacaagagtc tcccaaacag gcgcgagata aatcgagttt acaaactcaa
    18121 tgatgattgg ttcgcgaggg ttcactggca ggcgtgccac cactctccca gactctgcat
    18181 ccgataccac aaggcatgaa gtccgccagg ctgaacatcc atcaagcaat ggaatcaaac
    18241 agagaatcat gataaacgcc cagttgtaca ttatgcccat agcttttttg agagaataca
    18301 tcccgtcagg aacccgtgtc aagagtgaaa tggcatgaat ttctttagat tttttcgttg
    18361 acaaccagag gatgcttccg ctaagatggg gtatccaaaa ccagtgagga ggaatcatgg
    18421 ggaagatcag ggagatacca ctcgcggcat taaaactaaa tgagtttatc aagaataaag
    18481 ttaaggagat tgcagaggaa gtgaaagacg ggatcgcgat caatgccctg tccggtggtg
    18541 ttgattcctc ggttgtcaca ttgctgggtt tcaaggccct tggcggacaa ttgaaaacat
    18601 attttattga taccggactt atgcgtcatc aggaacctca atatgtagtc tcgatattta
    18661 aaagcctggg ggtacctgtg gaactcatca acgcaaagaa acaatttttc aacgccctta
    18721 aggggattga agatcctgaa aaaaaacgga atgcagtcac ccacgttttc tataaagaag
    18781 tttttggcga tctagtgaaa aaaagtggag caaaatacct gcttcagggg actaattaca
    18841 ctgatgttga ggaaacagtt gcaggaatta agagacagca caatatcctc acccagcttg
    18901 gtatagacac gaagaaagca tatggctacc atgtcatcga accggtgata gaactccgaa
    18961 aaacagcaat cagaaaagta gggagagcgc tgggcttacc aaaagagctg tatggaaggc
    19021 caccctttcc aggtcctgct ctttcagcac gggtgatcgg cgaggtaacg ccacaacggg
    19081 tggagattgt ccgtaaagcc acgaggattg ttgaggaaga attaggcaag acatccgctt
    19141 tccaatatct ggccatcctt catcaggaca gggtaaccgg catgcgcaat ggcaaacgtg
    19201 attttggtct tcagatcgaa gtacggtgct gggatagcaa agatgcacgc gaaggaactc
    19261 ccacaagact tccctttgac actttactga aacttgggga taggataacc ggtgaagtac
    19321 ccggcgttgt gagcgtcacc tataatataa cgaggaaacc accgtcaaca atagaagcca
    19381 tatagggaat caaggataat actaaaaaat aaaatggtgg ctcccatatc tcgggggcca
    19441 ccacatccac gctgttacaa tactttgtga aaactacttc tttgccgggg ctggcgctgc
    19501 tggcttcgcc tcggtttttg caggctccgc cttttttgca tctgccttct ttccggcgtc
    19561 tttcacttca acgcttacag ccttcatggt ataatggatg gtaaccttgg cacctacctt
    19621 cagctcacct gctacttttg tggctgcatc ctttgctatt tcccattttt ctttgccctt
    19681 ctgaacaacg atgatatcat ccttgatctc cagtaccggg cctgttactt gatatgtttt
    19741 cggtcctgct gcaaaagaaa atgatacaaa caccataact gcaaccattg acaacaaaat
    19801 ctttttcata agcacttccc tcctttttac ttgttgagcc atacggcttc gatagctcaa
    19861 ttcagaatat ttatttactt aactacaatg tagtctttga tcttgctgaa ggtaccttcc
    19921 ttaataccct tgaccttcat gacgtcctcc ggtactttgt atggccttcc atcgataatt
    19981 gcctgagctt taactggtcc cacatcgggc agggcttcaa gttcttcctt tgttgcggta
    20041 ttgatgttga tcttttgccc aggagcaagt ttcgaaggta cccttgcctt ttccctggca
    20101 tgagcttttg tagacacctt gactttctca gccggtgcct tcaccggagc ttcctttgcc
    20161 gcttctttta ctgccactgg tggagcagca ggtttagcag gggtcgcctg ttttgaatct
    20221 gatacagctt tggccggaac acctggcttt gtctccactg ccgtcggcgc tgctgcaggg
    20281 gcagcaccca cctttacata aggcttcatc gattcaatgg ttttatcagg aattttagcc
    20341 tttttcagat catctattga tttgtatgga cgaccggcta tgattttctt ggccgtagca
    20401 gggcctacac ccttgatttc ctcaagttct ttttctgatg cagtatttat gtctatgagt
    20461 gttttcgaaa cctgagcaca agcctctgta ataccaagaa aaaatacaag gccaatgata
    20521 ccggcaaaga aaatcaataa atgcattctt ctggtcctgc tcttcattgc acacttcttt
    20581 accttaggtt ttttatttca tgatttctta tcttttttac cattaaattt gaaaagactg
    20641 tatcagagtc attttacctt gtcaatcaca ataatttctt tgataatact ttctaaaact
    20701 caaataaggt catacctgac aaccgataaa atgttcaatt cttataaatt ctcagtaata
    20761 tgtttaaaac tcatacattc aatatcattg cgaaagtgag caccctgcac tgtttacagc
    20821 attaatagta atccattttt ttgttgccac aaccaggttg ttttacctat ggctatgcga
    20881 taataaacag acgggaaata gacgtatcta atctttcat
//
LOCUS       DAOOMC010000039        20409 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdus sp. isolate AMR_MDS_2685
            NODE_2581_length_20409_cov_6.924290, whole genome shotgun sequence.
ACCESSION   DAOOMC010000039 DAOOMC010000000
VERSION     DAOOMC010000039.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864760
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdus sp. (wastewater metagenome)
  ORGANISM  Syntrophorhabdus sp.
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae; Syntrophorhabdus.
REFERENCE   1  (bases 1 to 20409)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20409)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,729
            Genes (coding)                    :: 2,708
            CDSs (with protein)               :: 2,708
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 7 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20409
                     /organism="Syntrophorhabdus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2581_length_20409_cov_6.924290"
                     /isolate="AMR_MDS_2685"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2699756"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(293..1000)
                     /locus_tag="PK125_05550"
     CDS             complement(293..1000)
                     /locus_tag="PK125_05550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB37605.1"
                     /translation="MLPEYMTGKTGNKNQTKSKWPRILGIIALFFIVVITVSVWWIKH
                     NLYANPFNPTKLNEREQQVLNTKLERLEQSFQKQGKFSGTRTDEEMTKERLEPEDYTE
                     DPARREIRISQKELNALIAKDEKAARQIAINLSDDMISLKLIIPVDKDFPFLGGKTLR
                     LSCGVTLRHEAGKPVVALRGVSIGGIPIPNAWLGNLKNVDLVHEFNGQHGFWGTLSKG
                     VEDIKVSNGSLSIKLRE"
     gene            complement(1163..1801)
                     /locus_tag="PK125_05555"
     CDS             complement(1163..1801)
                     /locus_tag="PK125_05555"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016471 - vacuolar proton-transporting
                     V-type ATPase complex [Evidence IEA];
                     GO_function: GO:0008553 - P-type proton-exporting
                     transporter activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HPB37606.1"
                     /translation="MGKLNIAPTKSNLLVLKKQLTFAEEGYDLLEQKRQILIFELMSR
                     LARARDAEQGVAKALGLAFAALQEAQLEIGSDNIDRATLAVNMDHQVDVSDQHLMGME
                     IPHVTVQTEPVSIQFGIGGTSASTDVAMTRFIEVLPILAELAELENAIMRLAQELRKT
                     QRRCNALSKIFMPDYRETINYITGALEERERESFVILKMIRDRLGQSPSNNI"
     gene            complement(1913..3340)
                     /locus_tag="PK125_05560"
     CDS             complement(1913..3340)
                     /locus_tag="PK125_05560"
                     /inference="COORDINATES: protein motif:HMM:NF003235.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HPB37607.1"
                     /translation="MTITFDIEDRGLQYVGASRIEGPLVVVERVRDVGYDELVEILDP
                     HHVPRLGRVLEVSEHQAVIQVLEGTTGLSNKTVRVRFLGESFRLPVSQQMLGRIFDGL
                     GRPADGGPPPLSEDRRDVNGMPLNPNARRYPREFIQTGLSAIDGMNALVRGQKLPIFS
                     GNGLPHDRVSAQIVRQARLLEEEVQFSIVFAAMGVKHDVAEFFIRNFHDYGALGRAVM
                     FFSLADAPSVERLLTPRVALTLAEHLAFDCGQHVLVLLTDMTNYCESLREVGTARGEI
                     PGRKGYPGYLYSDLASIYERAGRIEGSQGSITQMAILTMPADDISHPVPDLTGYITEG
                     QVVLDRDLFQRGVYPPIAGLPSLSRLMKDGVGKGYTREDHPILASQLFACYAYVKRVR
                     GLADVIGEEELSTLDKQYLKFGEAFETRFLNQGEYENRSIETTLELGWDVLSTLPRDE
                     LHRVSDNLLNTYYREQEDRVKELSD"
     gene            complement(3337..5154)
                     /locus_tag="PK125_05565"
     CDS             complement(3337..5154)
                     /locus_tag="PK125_05565"
                     /inference="COORDINATES: protein motif:HMM:NF003220.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HPB37608.1"
                     /translation="MIAEEKTTQPVVTRISGPIVTAAGMHAAQMYEVVHVGSLGLVGE
                     VVRLIGDYATIQVYEDTTMLKPGAPVTCTGTPLSVWLGPGLVGNIYDGIQRPLPGIQA
                     RSGAWICRGEKVDPLNTEKVWTFEPLVSPGERIASGQPIGQVAETPLVNHLILAPPNC
                     DGTIRSIVDKGDYRLLDQLAVMETASGLRYITMLQQWPVREPRPIRERLRITEPLITG
                     QRIIDTFFPIGKGGAAAIPGGFGTGKTITQHQLAKWSNADIIVFIGCGERGNEMTEVL
                     REFPELKDPRSGMPLMERTILIANTSNMPVAAREVSIYTGITLAEYYRDMGLSVAVFA
                     DSTSRWAEALRELAARLEEMPAEEGFPASLPTRLAQFYERGGAVTTLGGNRASVSIVG
                     AVSPPGGDFSEPVTQHTRRFIRCFWALDTELANARHYPSIHWLHSYSEYVEDVGPWWK
                     KEAPDWVEMRTEALTLLQREDRLQQIVKLVGPDVLPEGQRLILFIAEIMKDGFLAQSS
                     FDENDMYCTPERQIALMRIILTLYHKGRDLIQRGVPLKQIRSLACVPQILRAKSVFSN
                     TDLDKLAELELQVKEDMDTLAKDYIKEATFDVDMHTTLL"
     gene            complement(5151..5801)
                     /locus_tag="PK125_05570"
     CDS             complement(5151..5801)
                     /locus_tag="PK125_05570"
                     /inference="COORDINATES: protein motif:HMM:NF014093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit E"
                     /protein_id="HPB37609.1"
                     /translation="MVTNQDSAEKLSTEILADARRKAAEAILRAKQESEAILNSAAVE
                     ADQVRQKILEEAGAEGRRRRELILATVPVEKGRLLAARVEALLDSIYDEARQRLHAHE
                     GFDYDEAVISLSSQAIRQMTGNKFVVRLSGKKPALADDSLAEQIVRRDGRSLHITISH
                     SENPPEDGVIIEDGEAHQVWDNRLLKRLERLWPELRRQIAVQTSLVHKPGAGESSQ"
     gene            complement(5814..6122)
                     /locus_tag="PK125_05575"
     CDS             complement(5814..6122)
                     /locus_tag="PK125_05575"
                     /inference="COORDINATES: protein motif:HMM:NF014092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HPB37610.1"
                     /translation="MKFYCIADEITARGFRLAGINAYVASTADQAQRGINDAVASTDC
                     AIIIITEKVASLVQSHIDMIRLEKDYPLIVEIPGPEGPLPGRKSLRELVQEAVGIKVG
                     "
     gene            complement(6133..6477)
                     /locus_tag="PK125_05580"
     CDS             complement(6133..6477)
                     /locus_tag="PK125_05580"
                     /inference="COORDINATES: protein motif:HMM:NF012365.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase subunit C"
                     /protein_id="HPB37611.1"
                     /translation="MIKSVLQRINMIGAVLVGIIFSATVVTGAETAGGAMPADQMSVY
                     KAIGLGVAAAIAISATVIGAAYAVGRIGSAALGAAAEKPELLTRSLLFVALAEGLAVL
                     GFAIAMMLIQKI"
     gene            complement(6543..8528)
                     /locus_tag="PK125_05585"
     CDS             complement(6543..8528)
                     /locus_tag="PK125_05585"
                     /inference="COORDINATES: protein motif:HMM:NF013647.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033179 - proton-transporting V-type
                     ATPase, V0 domain [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase 116kDa subunit family protein"
                     /protein_id="HPB37612.1"
                     /translation="MFRTVPMMRLQVLVLAQDERAVLSSLGQLGVVHLTHSNPGPDSA
                     PLEPADYTGELAHWDHLRTRIKELRQSLEIPPLNKKLGSLEGFTANHAEKSLFDLEKQ
                     SKDLVAHRQHIIQRQKDLANLSEQISCYRGYDIPLDGLDQFSFLHFATGRMPVQNLED
                     LDKELGENVVLLPLVKQNGQQPLLVITSSQDWPALEKKLYQSGFQQEFLPIVEGATVD
                     RVSEEDEKEQAKLAAELEELEGKIKTMAEEFALPLAEVERSVETEYRLLTAIQSFPRT
                     EAAVFISGWIPAEEVTTLEKHLGQITSGRYVLKVTPPDASTREQAPVLLKHSRLLRPF
                     EMLISTYGLPDYHELEPTLFVALSYIVMFGIMFGDAGHGLVLAAAGLTALIAGQSKKL
                     KDLGVLLLFAGFSSTIFGVVYGSYFGVETLKKYALWHDPLEGNPIQLMYGAIGVGIAL
                     ISLGLVLNVINRFQRGDVIGGILDKFGLVGLLFYWGTLALLLHGATIQSWGLMSVSVI
                     LFLVVPIVGWSFKEPLQHLIRHRAGGQKEMDGGLASAIMESCVGAFEAILSYLANTIS
                     FVRLAAYAMSHAALLFAAFMVSAEVRDLPFVGGAFSLSIIILGNIIAIVLEGIIASVQ
                     ALRLEYYEFFSKFFSGSGQPFAPFRLAADAKEYGKRV"
     gene            complement(8521..9633)
                     /locus_tag="PK125_05590"
     CDS             complement(8521..9633)
                     /locus_tag="PK125_05590"
                     /inference="COORDINATES: protein motif:HMM:NF014094.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HPB37613.1"
                     /translation="MRVQTAIDDSKFVQAISQNRQTENDLDYLVARLHGRRSRMAEAE
                     RLDNLCRIRNLHEFFRTIFPESDIKEVVGFQRQLIDEMVDEFSRFCSHLSGPGTDLLD
                     WMLVRFQVENLKVLIRACLTKAPLEEFKEYLISLPVKFTVNIQELTKAESPADFVHLV
                     PIRLLQEDLEKALEIYNKYPRSFFFEAALDCGYLKGLIEKTVHLSGEDCAFVRPMVFQ
                     EADIFNIMLVARGKFYYGLAPDILRQFYVPGTQISRTQFHSMLDEADLSSSMGRIPHC
                     VFDTDLPQFSREGGSIDVDASVLEGLAWNRFYRLANLAFRQSHMGLAAIVGYTGLRRV
                     ELANLIRISEGIRMDIPAETIRAHMIPRIGLEVKHV"
     gene            complement(9612..9926)
                     /locus_tag="PK125_05595"
     CDS             complement(9612..9926)
                     /locus_tag="PK125_05595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB37614.1"
                     /translation="MRDVIQKIISVENEANVTIQDARAEAERITSDARKKGNDLIENA
                     RQEALTEAKKIIDALIQDAEKEKQQQLASAAIEIEKRVRLDPAIREWAVEGVVRCVCK
                     QP"
     gene            complement(10015..10461)
                     /locus_tag="PK125_05600"
     CDS             complement(10015..10461)
                     /locus_tag="PK125_05600"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HPB37615.1"
                     /translation="MLVPTRILVPTDFSECSGRALRQALDIATEYQAKVFLLHVVHDV
                     MDFTGLDFIRSENMIQDFKGNELVWARRNLKKQLEGFPQANEVEVETVVRRGVPYVEI
                     LKLGEEERIDLIVIASLGKSGIARYLIGSVARNVLKGSKCPVLLTK"
     gene            complement(10961..11512)
                     /locus_tag="PK125_05605"
     CDS             complement(10961..11512)
                     /locus_tag="PK125_05605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB37616.1"
                     /translation="METRTNSVVKNEYDQILEFARAVVPHNWNVYWSWSWKEAKKIRH
                     TCNIQLHDDPFRDILEDGHLAMVHNMRWSDGIMFSDIYLDTYRFYSMEETILHELAHV
                     AEQRYIMLKDGSFREKDTTISGAFYRHSNTKKAGKSQRVFPFTGIYEDHGELFERCLS
                     AFRRRAKRKGKSLDLAKLFALPR"
     gene            12178..14439
                     /locus_tag="PK125_05610"
     CDS             12178..14439
                     /locus_tag="PK125_05610"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: protein motif:HMM:NF006417.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12-dependent ribonucleotide reductase"
                     /protein_id="HPB37617.1"
                     /translation="MNVYNITPKIAVPGNNVMDIDGTVLARLSKNAITVLEKRYLRRD
                     QNGQVMETAADMFLRVADAIASADKKFNNDADTAKLTEQFYSMMTNLEFIPNSPTLMN
                     AGRELGQLSACFVLPVGDSMDDIFDTVKHTALIHKSGGGTGFSFSNLRPKNDVVKSTT
                     GISSGPISFMKVFDIATETIKQGGTRRGANMGIMRVDHPDILDFIKCKSNQMNLNNFN
                     ISVGLTDSFMEAVRNDESYPLVNPRVKETSGMLRAREVFDLIVRQAWENGEPGIVFLD
                     RLNRDNPTPHVGTIESTNPCGEQPLLPYESCNLGSINLGLMVKDGDVDWERLKEKIHL
                     AVHFLDNVIELNRYPLPQIAKMTYANRKIGLGIMGWADMLIALGIPYNSEEAIKLGER
                     IMAFVNDEGHNASRELARERGPFPNFNGSVFSQRGEPPIRNATVTTIAPTGTISIIAN
                     TSSGIEPIFAVSFVRQVLDNNILVEVHPLFEMIARQRGIYSEALMEKIAEHGTIQDME
                     EIPADIRKIFVTAHDITPEDHIRMQAAFQRHTDNAVSKTVNFPKTATIDDVREVYELA
                     YELDCKGVTIYRDGSRENQVLSTAKTKQESVVDEKKSVRERPVSLRGWTYRMQTGCGP
                     LYVTINEDEEGLFELFTTMGKAGGCAASQSEAIGRMVSLAWRSGVQPEQVIKQLLDIS
                     CHSHAGFGDEKILSCADAVAKAIGKHLSSNGYIEKMEKRSLFKGACPECGGRVEHEGG
                     CAVCHSCGYSECI"
     gene            14551..14928
                     /locus_tag="PK125_05615"
     CDS             14551..14928
                     /locus_tag="PK125_05615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB37618.1"
                     /translation="MRQGKRLLIIYSACALFVYISGAGMIWADDTLSVRKNGDKTAYT
                     IGATDEKDNALGGKNDEEKAMYSIGPSKTKRDEKTKDKERSWDMLKNMGIAIDDRQSK
                     HHNIKNSQNQTNQDKQNKQAPMK"
     gene            15182..15724
                     /locus_tag="PK125_05620"
     CDS             15182..15724
                     /locus_tag="PK125_05620"
                     /inference="COORDINATES: protein motif:HMM:NF015944.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HPB37619.1"
                     /translation="MYGILVVVLVVIIGVFMYNRLIRSRNILNEAWSGIDVQLKRRHD
                     LIPNIVETVKGYVTHERKVLEEITNLRSRLAGPSTVQEKGQLENSFSQVLKSIFALAE
                     AYPDLKASQNFIELQRTLADAEEQIQLARRYYNGAARDYNTMVQSFPSNMMARIFGFS
                     KAEFFEIELATEREVPGVKF"
     gene            15752..17701
                     /locus_tag="PK125_05625"
     CDS             15752..17701
                     /locus_tag="PK125_05625"
                     /inference="COORDINATES: protein motif:HMM:NF021489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2207 domain-containing protein"
                     /protein_id="HPB37620.1"
                     /translation="MIKRIFFLFTVLFLNILVVVAVSIGQDRQKSERIISFDSNIHIY
                     RNAAMSVVETISVYSAGKEIKRGIYRDFPTKYKDRYGNSVVVDFRVNEVLRDGKPEEY
                     RLEGLRNGVRVYIGRKDVFLNPGRYTYTIRYSTNRQLGFFKAYDEIYWNVTGNGWSFP
                     IDRASATVRLPDVSVGEILEIDGYTGFKGDKGKDFFSSTRSPGTVLFQTTRTFKPGEG
                     LTVVVSWPKGYVTEPSIATKTQSFIRDNLGTFTSLFGLVVLFVYYFTAWFRHGKDPSK
                     GTIIPLYEPPDKFSPAAMRFITNMGFDDKVFAAAIINMAVKGYITIHEENNGYTLLKR
                     GEGDSLLSREEQVAASRLFGSSAKMVKVDSANQIAINGALVEMKRSLRNSLEKIYFFT
                     NKKYFIIGLCISVLIMIGGSVLGEKDGLPVAAFMSFWLTVWTFGVAILLMQVVSLWKS
                     VVSGGTHRRVLFFRAVFSSLFAIPFVVAEIGGIVALALAASVFIIVIMAVMVFINALF
                     YRLMKAPTIAGRKILDKIEGFKMYLSTAEKDRLNLLNPPDRTPELFEKYLPYAFALDV
                     EQEWSQQFSEVLSRAYASDTESSPQWYSGPSWHTKGISGFTSSFGDSLASAVSSASSS
                     PGSSSGGGGGGSSGGGGGGGGGGGW"
     gene            complement(18331..20004)
                     /locus_tag="PK125_05630"
     CDS             complement(18331..20004)
                     /locus_tag="PK125_05630"
                     /inference="COORDINATES: protein motif:HMM:NF011291.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004819 - glutamine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006425 - glutaminyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase/YqeY domain fusion
                     protein"
                     /protein_id="HPB37621.1"
                     /translation="MAPQSDFIREIIEDDFKTNKHKGRVATRFPPEPNGYLHIGHAKS
                     VCLNFGIAAQYRGTCNLRMDDTDPAGESLEYVESIINDVHWLGFDWENRLFYASDYFE
                     QLYQFAIQLIKDGKAYVDSLSADEIRRLRGTLTEPGKESPYRNRSVDENLDLFARMRA
                     GEFADGTHVLRAKIDMSSPNVTMRDPVIYRIKRAPHYRTGNNWVIYPMYDFAHCLSDA
                     IEKITHSICTLEFENNRPLYDWFVNELIADGRPNQIEFARLNLSYTMLSKRRLIELVE
                     KGYVSGWDDPRMPTVAGMRRRGYTPEAIRDFCARIGVAKSDNLVDIGLLESCVRDDLN
                     EHAPRVLGVLHPLKVIIDNYPDGQTEELECANHPQKPEMGTRRVPFSKVLYIEQEDFD
                     ENPPKKYKRLAPGKEVRLRSSYVIKHKNTVKNEKSGEITELHCTYDPETRNGPPPDSR
                     KVEGVIHWVSAEHAMPVEVRLYDRLFQVADPAASDQDFATLLNPNSLEILYNCYVEPG
                     LKKAIPGDRYQFERLGYFCIDTKDSSNGRPVFNRIVQLRDSWGKMSGKR"
BASE COUNT         5327 a         4780 c         4807 g         5495 t
ORIGIN      
        1 gaatggtttt gatgcttttg cacccggtat tcccaaacga gaaggctcgc cctccgacct
       61 tctccacctc tgtagagaac ttatctccta aactcaaagt ggcctgaact ttgtacacgt
      121 actttttcac gttttccttg agggtaacag ggatgagggt ttcagtatgg agggtacgaa
      181 ctcagctgcc tttctcctcc tatcgcgttt taagatagaa gatagtttat gccaggtgga
      241 tcatttttca tgaaaatatt agaccagttt agcatgataa tttacacaga aattactctc
      301 tgagttttat gctcaaggag ccgttggaga ctttgatgtc ctctacgcct ttagataggg
      361 tgccccagaa tccgtgttga ccattgaatt catggaccag gtctacattc ttgagattgc
      421 ccagccaggc gtttgggatg ggtatgccgc ctatactgac accgcgaagg gcaacaacag
      481 gttttccggc ctcatgacgt aatgttacac cacagctaag tcgtaatgtt ttgcccccaa
      541 ggaaggggaa atctttgtct acagggatta tcagcttgag gctgatcata tcgtcggaca
      601 ggttgatggc aatctgacgg gcagctttct catcttttgc tatgagcgca ttgagttctt
      661 tttgtgagat tcgaatttcc cgccttgcag gatcctctgt gtaatcttca ggttcgagac
      721 gctctttagt catttcttca tcagtccttg tgccggagaa ctttccttgt ttttgaaagc
      781 tctgctccag gcgttctagc tttgtattca gcacctgctg ttctctttca ttcagcttgg
      841 tagggttaaa cgggttggca taaaggttgt gttttatcca ccagacactc actgttatca
      901 ctacgatgaa aaatagcgct attataccca gtatgcgagg ccatttcgat tttgtttgat
      961 ttttattgcc tgttttccct gtcatatatt ccgggagcat aaaaatcctt ttacaatctc
     1021 atctttcaac aaaagttaca aaattcattt tattcttttc tgcatgccac aatgtgtacc
     1081 acactattct atacctgtga gaatgagctg taaagaccat ccaatattat gagattctat
     1141 atgatatggc tgcaggtcgt tatcaaatgt tattggatgg tgattgcccg agcctatccc
     1201 gtatcatttt cagaattaca aaggactccc gctccctttc ttccagagcg cctgtgatat
     1261 agttgatagt ctcccggtaa tccggcatga agatctttga gagtgcgttg caccgtcgct
     1321 gggtcttccg aagctcctga gcaaggcgca tgatagcatt ttccagttct gccagttcag
     1381 caaggatggg aagaacctcg ataaaacgtg tcatggccac atcagtgctt gctgaagttc
     1441 ccccgatgcc aaactggata cttaccggct ctgtctgaac cgtcacatgg gggatctcca
     1501 tgcccataag gtgctggtca gagacatcca cctgatggtc catgttaaca gcaagggtgg
     1561 cacggtcgat gttgtctgat ccgatctcca gctgggcttc ctggagggct gcaaaagcaa
     1621 ggccaagggc tttagcaaca ccctgttctg catcacgagc gcgtgcaagg cggctcatca
     1681 gctcaaagat aagtatttgt cttttttgtt cgaggaggtc gtaaccttct tcagcgaaag
     1741 taagttgttt tttcaatacg agcaaattcg atttggttgg agctatgttc agttttccca
     1801 taaaaccctg ccattttccg gagctcacac ctgcctgtgc tatatgcttc agtaggcacg
     1861 tgatcatccc ttttcacgaa attagtctga tagattggct aatccttatc cattaatcac
     1921 ttaattcctt aaccctgtcc tcctgttccc gatagtatgt gttcaacagg ttgtcgctaa
     1981 cacggtgcaa ctcatccctg ggcagcgtgg agagtacatc ccagccaagt tcgagtgtag
     2041 tttcgataga tcggttctcg tattcaccct ggttcaaaaa gcgtgtttca aaagcttcac
     2101 cgaatttcag gtattgtttg tcgagagtgc tcagctcttc ttcgccgatg acatcagcca
     2161 gccctcggac ccgcttgaca taggcgtagc atgcgaataa ctggcttgca agaatgggat
     2221 ggtcttcccg ggtgtatcct ttgccaaccc cgtctttcat caaacgggaa agactcggaa
     2281 ggccggcaat aggaggataa acgccacgct gaaacaaatc tctgtccagg acaacctgtc
     2341 cttcagtaat gtagccggta agatccggaa caggatggct gatatcatct gcaggcatgg
     2401 tgagaatggc catttgagtg atggaaccct gtgaaccctc gatgcggccc gcgcgctcgt
     2461 aaatgctggc aagatcagag tacagatagc cgggataccc ttttcggcct ggaatctcac
     2521 ctctggcagt accgacttcc cgcaaacttt cacagtaatt tgtcatgtca gtcaagagca
     2581 caagcacatg ctgtccgcag tcaaaagcca gatgttctgc gagggtcagg gcaacacgag
     2641 gggtgagaag acgttcaacg cttggtgcat ctgcaaggga aaagaacatg acggcgcggc
     2701 caagagcacc atagtcatga aagttgcgga tgaagaattc agctacatcg tgctttacac
     2761 ccatggcagc aaagactatt gagaactgaa cttcctcttc cagcaggcgg gcctgtcgca
     2821 caatctgggc tgatacgcgg tcgtgaggaa gcccgtttcc cgaaaagatg ggaagtttct
     2881 gaccacgaac cagtgcattc atcccatcga tagctgataa gcctgtctgg atgaattcac
     2941 gcgggtaacg cctcgcatta ggatttaggg gcattccatt gacgtctcgc ctgtcttcag
     3001 agagcggagg cgggccccca tcagccgggc ggccgagacc atcgaatatg cgtccaagca
     3061 tctgctgaga aacaggaagg cgaaagctct ccccgaggaa acggacccgc actgttttgt
     3121 ttgagaggcc tgttgtgcct tcaagcacct ggattacggc ctgatgttca gatacttcga
     3181 gaacacggcc gagacggggc acatggtgtg ggtcaaggat ctcaacaagc tcatcatagc
     3241 ctacatcccg aacacgctcc accacaacca gagggccctc gatgcgcgaa gcccctacgt
     3301 actgtaagcc cctgtcttct atgtcgaatg tgatcgtcat aacaaggtgg tatgcatatc
     3361 tacgtcgaaa gtagcttctt tgatatagtc ttttgccagt gtgtccatat cttccttcac
     3421 ctgcagttcc agttccgcga gcttgtccag gtctgtgtta ctgaataccg acttggcccg
     3481 aagtatttgt ggtacacagg ccagggagcg aatttgcttt aacggcaccc ctctttgaat
     3541 taaatcacga cccttgtgat agagagtaag gatgatgcgc atgagggcaa tctgtctttc
     3601 cggagtgcaa tacatatcgt tttcatcgaa agagctctga gcgaggaaac catctttcat
     3661 tatctcggct atgaaaagga tcaaacgttg tccctcaggc aggacatcag gtccaacgag
     3721 cttgacgatt tgctggaggc ggtcctcacg ctggagaaga gtgagcgcct ctgttctcat
     3781 ttcaacccag tcaggagcct ctttcttcca ccacggtcca acatcctcca catattcgga
     3841 atacgagtgc agccagtgga tagatggata atgccgggca ttggcaagtt cggtgtcgag
     3901 ggcccagaaa catcgtatga atcggcgtgt gtgctgggtg accggctcgg agaaatcacc
     3961 acctggcggg ctcaccgcac ccacgatgct taccgatgcc cgattgcctc ccagggttgt
     4021 aacggccccg ccgcgctcat aaaactgtgc cagtcgtgta ggcaatgacg cggggaagcc
     4081 ttcttccgct ggcatctctt ccagccgagc tgcaagttcc cgcaacgctt ctgcccagcg
     4141 gctggtggaa tctgcaaaaa cagcaacgga tagtcccatg tcccgataat actctgcaag
     4201 ggtaatgccg gtatagatgg atacttcacg tgctgccacc ggcatattgg aggtgttggc
     4261 tatgaggatg gtcctctcca tgagcggcat gcccgacctg ggatctttaa gctcaggaaa
     4321 ctctcgaaga acctcggtca tttcatttcc ccgctcgccg caaccgatga agacaatgat
     4381 atcagcattt gaccattttg caagctggtg ctgggtgatc gttttccctg tgccaaatcc
     4441 tccgggtata gcagcagcgc ctcccttgcc aatgggaaaa aaagtgtcga taatacgctg
     4501 acctgtaatc agcggttctg tgatacgcaa gcgttcccgg atggggcgag gctcccgcac
     4561 gggccattgc tgaagcatgg ttatataccg caggccagat gcagtctcca tcacagccag
     4621 ttgatcgagg agtctataat cccctttgtc gacaatcgat cttatcgtac cgtcacagtt
     4681 tgggggagcc aagatcaggt gattgaccag tggtgtctct gcaacctgcc caatgggctg
     4741 gcctgatgca atgcgctcgc ctgggctgac gagcggttca aaagtccata ctttttccgt
     4801 attcaaggga tcgacctttt ctccgcgaca gatccatgcc ccgctacgtg cttggatacc
     4861 tggcaagggt cgctgaatac cgtcataaat gttgccaacc aggccagggc caagccacac
     4921 agacaaagga gtgcccgtgc atgtgaccgg agctccgggc ttcaacatgg tagtatcctc
     4981 atacacctga atagtggcat aatcgccgat gagtcgtacc acttcaccca ccagcccaag
     5041 actaccaaca tggactacct cgtacatctg ggctgcatgc atacccgcag ccgtgacgat
     5101 ggggccgctg atacgggtga caaccggttg agtagttttt tcttcagcga tcattgactg
     5161 ctctctcctg ctcctggttt atggacaagc gacgtctgta cagctatctg cctgcgcaac
     5221 tctggccaca atcgttccag tcttttcaga agacgattgt cccagacctg gtgagcctca
     5281 ccatcttcta tgatgacccc gtcctctgga gggttttccg agtgtgaaat agtgatgtgt
     5341 aacgatctcc cgtcacggcg tacgatttgc tcagcaagac tatcgtccgc aagtgcgggc
     5401 ttttttccag agagcctgac cacaaattta ttgcctgtca tctggcgtat tgcctgcgaa
     5461 gagagagaga ttactgcttc gtcataatca aaaccctcgt gggcatgcaa tcgttgacga
     5521 gcttcatcgt agatagaatc aaggagtgct tcaacacgtg ccgcaagcag cctgcctttc
     5581 tctacaggga cggtagccag aatcagttcc ctccgacgcc taccctcggc cccggcctct
     5641 tcaagtatct tttgcctgac ctgatctgcc tcgacagcag cgctgttcaa gatggcctca
     5701 gactcttgct tagcacggag gatagcttct gcagcttttc tgcgagcgtc tgcaaggatt
     5761 tctgtgctaa gtttctcagc ggagtcttga tttgtcacca tgatcagctc ctcttatccg
     5821 acctttatgc ctactgcttc ctgtacgagt tcgcgcaggc tcttgcgtcc tggcagaggc
     5881 ccctccggtc caggtatctc gacaatgagc ggataatcct tttccagtcg aatcatatct
     5941 atgtgtgatt gaaccaagga agccaccttt tcagttatga taattattgc acagtcagta
     6001 ctggcaactg catcgttgat acccctctgg gcctgatccg ctgtcgatgc aacatatgca
     6061 ttgatacctg ccaagcgaaa ccctctcgct gttatctcgt cggcaatgca atagaatttc
     6121 acagtgaacc tcttaaatct tttgtatgag catcatggca atagcaaagc caagcacagc
     6181 tagcccctct gccaatgcca cgaacaggag gcttcgagta agaagctcag gtttctccgc
     6241 tgctgcacct aatgctgcag acccaattcg tccgactgca taggcagcgc cgataacggt
     6301 ggcagaaatg gcaatggcag cagcaacacc aagaccgatg gctttgtaca cggacatctg
     6361 atctgcaggc atagctccgc cggcagtctc tgctcccgta accaccgtag cagagaaaat
     6421 tatacctacc agaactgctc cgatcatatt aattcgttgt aagaccgact ttatcatgca
     6481 acacctccat ttctgaggtt tacgctgtta cctccatgaa gaaagtccat agatcctctc
     6541 cttcacaccc gtttcccata ctccttggca tctgccgcaa ggcgaaaagg cgcaaatggc
     6601 tggccgctgc ccgagaaaaa tttactgaag aactcataat attcgagacg caacgcctgt
     6661 accgaggcta tgatcccttc cagtacaatg gctattatgt tgcccaatat aatgattgat
     6721 aggctaaagg ccccacccac aaaaggaaga tctctgacct cagcggaaac cataaaagcg
     6781 gcgaagagta atgcggcatg gctcatggcg taagccgcaa gacgtacaaa acttatggtg
     6841 tttgcaaggt agctgagaat tgcttcaaat gcaccaacac aggattccat gatggcgctt
     6901 gcaagtccgc catccatctc cttttggcca ccagctctgt gtctgatgag atgctgtaac
     6961 ggctccttga aggaccagcc tactatgggt actacaagga acagaatgac tgatacactc
     7021 ataagccccc atgattgaat ggttgctccg tgcaatagaa gggctaatgt tccccagtag
     7081 aaaagaaggc caacgaggcc gaacttatcg agaatgccac cgatcacatc acctcgctga
     7141 aagcgattga tgacattcaa cacgaggccg aggcttatca atgcaatacc aacgccaata
     7201 gcgccataca tcagttgtat tgggttgccc tccaagggat catgccacag agcgtatttc
     7261 tttaacgttt ccacgccaaa ataacttcca tacaccacgc caaaaatggt acttgaaaaa
     7321 cccgcaaaaa gaagaagcac gccaaggtct ttcaattttt tggactgccc ggcaatgagt
     7381 gcggtcaaac ctgctgccgc aagaaccagc ccgtgtccgg catcaccgaa cataataccg
     7441 aacatcacta tgtaacttag tgcaacaaag agggtcggct ccaactcatg gtagtcaggc
     7501 agtccatagg tagaaatcag catctcaaag ggccgcagaa ggcgagaatg cttcagcaga
     7561 accggagctt gttcccttgt agaagcatcc gggggagtaa ctttaagcac ataccgaccg
     7621 ctggtgattt gccccaaatg tttctccagg gttgttactt cttctgctgg aatccaccct
     7681 gaaataaaaa cagctgcctc cgtgcgtgga aaactctgga tcgcagtgag gagcctgtat
     7741 tctgtctcca cagatctctc aacctcagcc aggggcaatg caaattcctc agccatggtt
     7801 ttgattttac cttccagttc ctccagttcc gctgccagct tcgcctgttc tttctcatct
     7861 tcttctgaca ccctatccac cgttgcgcct tcaacaatag gaaggaactc ctgctgaaag
     7921 cccgactgat acagtttttt ttccagagca ggccaatcct gggacgaggt gatcacaagc
     7981 aggggctgct gtccattttg tttaaccaac ggaaggagaa caacgttttc gcctaattct
     8041 ttatcaaggt cttcgaggtt ttgaaccggc attcttccag tggcaaaatg aaggaaagaa
     8101 aattggtcaa gcccatccaa tggaatatca tatccacggt aacatgatat ctgttcggac
     8161 agattggcta aatctttttg gcgctgaatt atatgttggc ggtgtgccac caggtctttg
     8221 ctctgttttt ccagatcaaa cagacttttt tcagcatgat tcgctgtaaa tccttccagc
     8281 gaacctaatt tcttattcaa aggagggatt tcaagagact ggcgaagctc cttaatgcgg
     8341 gttcgaaggt ggtcccagtg tgccagttct cctgtataat cggcaggctc cagaggcgca
     8401 gagtctggac cgggatttga gtgggtaagg tgcacaaccc cgagttgacc cagactgctg
     8461 agaacagcac gctcatcctg ggcaagcaca agtacctgga gtcgcatcat gggcactgtt
     8521 ctaaacatgt ttaacctcta agccaatccg tggaatcata tgagcgcgga ttgtttctgc
     8581 aggtatatcc atccggatcc cttcggaaat cctgatgagg tttgccagtt ccacacgacg
     8641 aagacctgtg taccccacaa ttgccgccaa tcccatgtgg ctttgacgaa aggccagatt
     8701 agccagacga taaaatcgat tccatgcaag accttccagg actgaagcgt caacatcgat
     8761 ggatcctccc tcgcggctga attgaggcag atcagtgtca aatacgcaat gagggatgcg
     8821 gcccatggag gaagaaagat ctgcctcatc cagcatggag tggaattggg tgcgggaaat
     8881 ctgtgttccg gggacataaa actgacgtaa tatatcggga gccaggccat agtaaaactt
     8941 tccacgagca accagcataa tattgaagat atctgcctcc tgaaaaacca tgggccttac
     9001 gaatgcacag tcttcccctg aaaggtgcac tgttttttca atcaaccctt taagatagcc
     9061 acagtccagg gctgcctcaa agaagaagga tcggggatat ttattgtaga tttcaagggc
     9121 tttctccaga tcctcctgga ggagtcttat tggaaccaga tggacaaaat ctgcaggaga
     9181 ttctgctttt gtcaattcct ggatattcac ggtaaatttg acaggaagag agatgagata
     9241 ttccttgaac tcttctaacg gggcttttgt caggcatgct cggattaata ccttcaggtt
     9301 ttccacctgg aatcttacca acatccaatc gagcagatct gttcccgggc cagacaggtg
     9361 cgaacagaag cgagaaaact catctaccat ttcgtcaatt agctggcgtt ggaaaccgac
     9421 tacttctttg atatcagact ctggaaagat tgtgcgaaag aattcatgaa ggtttcgaat
     9481 acggcacaaa ttgtcgaggc gctctgcttc tgccatgcgg ctccgtcgtc catgaagtcg
     9541 cgcaacaaga tagtcgaggt cattctccgt ttgtctgttc tgagaaatag cctgaacaaa
     9601 ctttgagtca tctatggctg tttgcacacg caccggacaa ctccttcaac ggcccattcc
     9661 ctgatggctg gatcaagcct gacccgcttt tctatttcaa tagcagcact ggccaattgc
     9721 tgttgttttt ctttttctgc gtcctgtatc agggcatcaa tgattttttt tgcttcagtg
     9781 agcgcctcct gacgggcatt ttcaataaga tcattacctt ttttccgtgc gtccgaagtg
     9841 attcgttccg cctcggccct tgcatcctgg atggtcacat ttgcttcatt ttctactgag
     9901 atgatctttt gaattacgtc acgcatgaca gcacctcccg aaccctccct gcattatatg
     9961 agtcgagttg ctacccatgc tggattggca tctcttctgt accccaacat ctttctattt
    10021 agttaacaac acagggcact tggagccctt cagaacattc cgcgccacac ttcctatcag
    10081 gtatcgcgca atgccagatt tcccgagaga tgcgattaca atcaggtcga tcctttcctc
    10141 ctcgcctaat ttgagaatct ctacataggg aacgcctcgg cggacaacag tttccacctc
    10201 cacctcattc gcctgcggaa agccctctag ctgtttcttc aaatttcttc ttgcccacac
    10261 gagctcgtta cccttgaaat cctgaatcat attttcagag cgtataaaat cgagaccggt
    10321 aaaatccatc acatcatgga cgacgtgaag aaggaaaacc ttggcctgat attcagttgc
    10381 aatgtccagt gcctgcctca atgctctccc tgagcattct gaaaaatctg ttggtacaag
    10441 aattctggtt gggacaagca tttttaatcc tcctttgacg tacccctatg ggatataaac
    10501 actacagtaa ctccctatga attgagtgta ttgtgaccaa ctatggtaga ggctaaggaa
    10561 gagcttacaa ggaaagccct ttgttacaca aaatatggga actccatttt tcattttgaa
    10621 ccaaaagtct tgttaagggt tcattcagcg tgggctacgg gctgggaacg ttgagcacga
    10681 gagaaaccaa caggggtctc tatgggtcaa tcattctgct tggaatatta attaaccttt
    10741 ctaatcacta cttatagcac aaattccatc tttgtcaatc gagtgtacag tggaaccaca
    10801 ccttgaagcg tggttgaata tgtttgctat tcgtgaataa tgtgtatgct ttgatgctca
    10861 gggcttgctc gtgggaatct atggatgacc gggctgttaa gaaggggcgt ggaaggattt
    10921 ttatatctcg gttgaattgg agatcgaatg atgtgtctga tcagcgtgga agggcgaata
    10981 gttttgccag atccagcgat ttacccttcc ttttcgcgcg acgacggaag gcgctgaggc
    11041 atcgttcaaa caactctcca tggtcttcat agatgccagt gaagggaaat acccgttgtg
    11101 atttaccagc tttttttgtg tttgaatgcc ggtaaaacgc gccagatata gtggtatcct
    11161 tttcccgaaa gctaccgtcc ttcaacatta tataacgttg ttctgccaca tgggccagtt
    11221 catgcaatat agtttcctcc atactataga aacgatacgt gtccaaatat atatcagaaa
    11281 acatgattcc gtcagaccag cgcatattat ggaccatggc aagatgacca tcttccagga
    11341 tgtcacgaaa gggatcatcg tggagctgaa tattacaggt gtggcggatt ttttttgctt
    11401 ctttccatga ccagctccag tatacattcc agttatgcgg taccacagca cgggcaaact
    11461 ccagaatttg atcgtattca tttttcacta ctgaattagt tctggtttcc atacaaaagc
    11521 ctcctttaac gaatagcgtt cgacaaagga tttttgacgc gatggcaaag aataaaaatt
    11581 gctgattttg tggcatatca tatactcgtg aatcatgata taccgaagat aatcattaag
    11641 ataatggtgg tgagcagata ccagccaatt gcccatgtaa tcagaaggct caccttttgt
    11701 ggtgatttct tcatgtctgc cccctccttt attattagga gttattgata cccggatgac
    11761 agttgatgtg tttgacagtg gagcgatctg gcgcaatagc ttctcctttt acctacccct
    11821 cgccgatcct accgtgtttg atatgtctga atctattagc aaattgcata ccgtttttac
    11881 taccaccaca ggagcgctat tttacagcac tttttgttta ttgttctgca acatgacaaa
    11941 gagcagggct acaaccttca gtcgctctct acaagatttg ttggactgat tccaagtgaa
    12001 cagaccagca acttctcaga taaaggcagg gataaatttt atctcttata ttctttgtac
    12061 tttgtacagg ttccaacttt aagaaaaatt gtttcaagat ttacagagat ttctgttgac
    12121 gagtcctata tatagtggta atagttttat tctacacaat atatagtgag gcatgacatg
    12181 aatgtgtata atataacccc taaaatagcg gtgccaggaa ataacgtcat ggatatagat
    12241 gggaccgttc ttgcccgttt gtcaaaaaat gctataactg tcttagaaaa gcggtacttg
    12301 cgaagggacc aaaacgggca ggttatggaa acagccgcag acatgttttt gcgtgttgcc
    12361 gatgccattg cctctgctga caagaagttt aataatgatg ctgatacagc aaagctgaca
    12421 gaacagttct attccatgat gacaaatctc gaattcatac ccaactctcc caccctcatg
    12481 aatgcaggtc gcgaattggg acaactgtcc gcatgctttg tgctacctgt tggggattca
    12541 atggatgaca tattcgatac agtcaaacac acagcattga ttcataagtc cggaggaggt
    12601 actggctttt ctttctctaa tcttcgtccg aaaaatgacg tagtcaagtc aacgaccggt
    12661 atttcaagcg gacctatatc ttttatgaaa gttttcgaca ttgcaactga gacgatcaaa
    12721 caaggtggaa ctcggcgtgg ggcaaacatg ggaatcatga gagttgatca tcctgacatc
    12781 ctcgatttca ttaagtgtaa atccaaccag atgaatctga acaatttcaa catctcagtt
    12841 ggcctgacag actccttcat ggaagcggtg agaaatgatg aatcctatcc attagttaat
    12901 ccaagagtca aagaaacaag tggcatgctg agggctcggg aagtttttga tcttatcgtt
    12961 cgacaggcat gggaaaacgg agaacctggc attgtttttc ttgacaggct aaaccgagat
    13021 aatccaactc cacacgttgg caccatagaa tcaactaatc cctgtggaga gcagcccctg
    13081 cttccttatg aatcatgtaa tctgggatca atcaacctgg gtttgatggt aaaagatggt
    13141 gatgtagact gggaacgact gaaggaaaag atacacctgg cagtccattt tttggacaat
    13201 gtaatagagc ttaaccgcta tcccttgcca cagattgcca agatgaccta tgcaaaccga
    13261 aagatcggcc tgggtattat gggatgggca gacatgctta ttgcccttgg gattccctac
    13321 aattcagaag aggcaatcaa actgggcgaa agaataatgg cctttgtcaa tgatgagggc
    13381 cataatgcct ctcgagaact ggccagagag agggggccat ttcccaattt taacggttca
    13441 gtattttctc aacgaggtga gccgcccata aggaacgcaa cggtcaccac gattgcccct
    13501 acgggtacaa tctctatcat tgccaatact tcatcaggta ttgagcccat ctttgcagta
    13561 tcgttcgtgc gccaggtgct tgacaacaat atcctggttg aagttcatcc cttatttgag
    13621 atgatagcaa ggcagagggg gatctattcc gaggccctca tggagaagat agctgagcat
    13681 ggaactattc aggacatgga agagatccct gcggacattc ggaaaatatt cgttacagcc
    13741 catgacataa cccctgagga tcatatccgc atgcaggctg cattccagag gcacactgat
    13801 aatgctgtta gcaagacggt aaattttccg aagaccgcca ctattgatga tgtacgggag
    13861 gtttatgagc tcgcctatga actcgattgc aagggcgtca ccatatatcg tgacggatcg
    13921 agagaaaacc aggttctttc tacagccaaa acgaagcagg aatcagttgt ggatgagaaa
    13981 aaatctgtcc gtgagcgccc tgtctccttg agaggatgga cgtataggat gcagaccggg
    14041 tgcgggccct tatacgttac tatcaatgaa gatgaggagg ggctgtttga gttgttcact
    14101 accatgggaa aagctggggg atgtgctgcg tcacaaagcg aagccatagg caggatggtt
    14161 tcgcttgcct ggagaagtgg agtgcaacca gagcaagtca taaaacaact cctggacatt
    14221 tcctgtcatt cccacgccgg ttttggcgac gaaaagatac tatcctgtgc agacgcagtg
    14281 gcaaaagcga ttggaaagca tctgtcctct aacggatata ttgagaaaat ggaaaaaaga
    14341 tcgcttttta agggtgcgtg cccggaatgt gggggaaggg ttgaacatga gggtggctgt
    14401 gctgtgtgcc attcgtgcgg ttacagcgag tgtatttaat cgagctaaat tgtccaacta
    14461 aaagattatt gtgaatcgaa taagggtcga taatacctgt aacgataaag ttttgatgaa
    14521 aagaagatcg acatggaagg gtgttccaag gtgagacagg gaaaacgttt gctgatcatc
    14581 tactctgcct gtgctctttt tgtgtatata tcaggggctg ggatgatttg ggcggacgat
    14641 accttaagcg tcaggaagaa tggtgataaa acagcatata ctattggtgc taccgatgaa
    14701 aaagacaacg ctttgggtgg gaagaatgac gaagaaaaag ccatgtattc tatcggaccc
    14761 agcaaaacca aacgagatga aaaaacaaaa gacaaagaaa gatcatggga tatgctcaag
    14821 aatatgggta ttgcaataga cgataggcag agtaagcacc acaacattaa aaattcacag
    14881 aaccaaacca atcaggataa acaaaataag caagccccaa tgaaatgaaa tcttctggta
    14941 atcaagaagg tgggttcaat gatatggaaa agagattccg tgtcattgcg agcaaggtga
    15001 agtgatctga aactttagat tgccatgtcc tcttctcagc gcaatcctgc gacgggaagg
    15061 cacagcaccg tgtttcttgt aatggcaaaa atgcgccttt tttgattggc agtttatatt
    15121 gacagggcaa acgtaaaagt gaaatactga aatacgttac aatcccggga gggccaaacc
    15181 tatgtatgga attcttgtag ttgttttggt ggtgatcatt ggcgttttta tgtacaatcg
    15241 tcttatacgt tcacggaata tcctcaatga agcgtggagc ggtatcgatg tgcaacttaa
    15301 acggcgtcat gatttaattc ccaacattgt tgagacagta aaaggctatg tgacgcacga
    15361 aagaaaggtt ctggaagaga ttaccaatct tcgttcacgg cttgccggcc cttcaacggt
    15421 tcaggaaaag ggacaacttg aaaatagttt ttcccaggta ttgaaaagca tatttgccct
    15481 tgcagaagcc tatcctgacc ttaaggccag tcagaatttc attgaacttc agcggaccct
    15541 tgcagatgca gaagaacaga ttcagctcgc acgaagatat tacaatggag ctgccaggga
    15601 ttacaacacc atggttcagt cctttccgag taatatgatg gcccgtatct ttggcttctc
    15661 aaaagccgag ttttttgaga ttgaacttgc tacagaacgg gaagtacccg gtgtcaaatt
    15721 ttgactaatt gacaaccgta ttgtaggcat catgataaaa agaatctttt tcctttttac
    15781 ggttttgttc ttgaacatcc ttgtagttgt ggcagtttct atcgggcaag atcgccagaa
    15841 atcggaaagg ataataagtt ttgatagcaa tatacatatt tacaggaatg ctgcaatgtc
    15901 ggttgttgag accatatcgg tttacagtgc gggtaaagaa ataaaacgcg gtatttaccg
    15961 ggattttccc acaaaataca aagacagata tggcaatagt gttgttgtcg attttagagt
    16021 aaatgaggtt ctgcgggatg gaaaaccgga ggaataccgt ctggagggct taaggaacgg
    16081 tgtgcgcgtt tatattggca ggaaagatgt ttttcttaat cccggcaggt atacttacac
    16141 gatccgatat tcaaccaaca ggcagcttgg ttttttcaag gcttatgatg agatctactg
    16201 gaatgtgaca ggtaatggat ggtctttccc tatagacagg gcttcagcaa ctgttcggct
    16261 tcctgatgtg agcgtgggag agatattgga aattgacgga tataccggtt tcaagggtga
    16321 caagggaaaa gattttttct cctccacccg cagcccggga acagttcttt ttcaaactac
    16381 acgaacattc aagcccggtg aaggcttgac tgttgtagtt tcctggccta agggatatgt
    16441 aacagaacca tcaatagcta caaagacaca atcctttatt cgtgataatc ttggtacttt
    16501 taccagtctc tttggcctgg ttgttttgtt tgtttattac ttcactgcat ggttccgtca
    16561 tggtaaggat ccttccaaag gaacgattat tccactttat gagccgccgg ataaattctc
    16621 tcctgccgcg atgcgtttta taacgaatat ggggtttgat gataaagtat tcgcagctgc
    16681 aatcataaac atggcagtga aaggatacat aaccatccac gaagagaaca atggctatac
    16741 ccttttaaaa aggggagaag gagattcact gctttcccgg gaggaacagg tggcagcatc
    16801 aaggctattc ggatcatcag ccaaaatggt aaaggttgat agtgcaaatc agatcgcaat
    16861 caatggtgcc ctggtagaaa tgaaacgttc tttgcggaac agtcttgaga aaatctattt
    16921 ttttacaaat aaaaagtatt ttattatcgg actgtgcatt tcggtcctta ttatgatagg
    16981 tggcagcgta ttaggagaaa aagatggatt gcccgtggca gcattcatgt cattttggct
    17041 gactgtttgg acttttggcg ttgcgatcct ccttatgcag gttgtttctt tgtggaaaag
    17101 cgttgtttcc gggggaaccc atagacgggt gctttttttc agggctgttt tctcgagttt
    17161 atttgccatt ccttttgtgg ttgccgagat cgggggcatt gtggcgcttg ctcttgccgc
    17221 gtcagtattt atcatagtga tcatggcagt catggtcttt ataaacgctc tcttctatcg
    17281 gttgatgaaa gccccgacca ttgcgggaag aaagatcctt gataagattg aagggtttaa
    17341 aatgtatctc tccacagccg aaaaagatcg tctcaacctg cttaaccctc ctgacaggac
    17401 acctgaactt ttcgaaaaat acttgcccta tgcctttgct cttgatgtgg agcaggaatg
    17461 gtcccaacag ttttccgagg tattgtcacg ggcatatgct tcagatacag aaagctcacc
    17521 tcagtggtat tccggtccat catggcatac aaagggtatt tcagggttca cctccagctt
    17581 cggtgattct ctggctagtg ctgtttcttc ggcttcatct tctcctggct cctcttcagg
    17641 cggcggcggt ggtggctctt ccggtggagg cggtggcggt ggcggcggtg gtggatggta
    17701 agatatccgt gtatgaaagt attgtctttc tgtacctctt gtttttttgg agattttatt
    17761 gaaaaacact acctgtcatt ttttttgtgt tgatcttttt cctcactgta gtgattcacg
    17821 gaatagcagt gactatccgg ttaatgggat ctgatgacat gaatggtaca gtgaacagca
    17881 tcagttggtt acgatgaaaa attaaggctg ttagtggaat gtccggaagt accggcagag
    17941 ataatatcat tccagctcat ttttgcctca ttgtcagatt ttgaagacgt tgaccgtcaa
    18001 actgctgtgg ctatgacaag atgccttgac tgattcgcat tcaaagatcc gaaaaaagta
    18061 gcaggccatc gtttattctt ttgaatatca acaacaatga caagaaaaat tcaaaaaagg
    18121 tataaaaatc agggtcagtg gctacaccgt tcatggagat gaaggaagga cctggacaca
    18181 cttcagacga accaatatca tgaaaaacag taaattgcca tccacggtaa agtacccaca
    18241 ggcaatattc attctatgac gggcttgaca tttgtcatac ccatcaagtt aaagcgtgag
    18301 atggaatctc tcacatgcat tgttatgctc ttatcttttt cctgacattt tgccccagga
    18361 atcacggagc tgaacgattc ggttaaaaac aggccgtcca ttgctggagt cttttgtgtc
    18421 aatgcaaaaa taccccaacc gttcaaactg atatctgtcg cctggaatgg ctttcttcaa
    18481 acctggttct acataacagt tgtacaggat ttcaagggag ttagggttta aaagcgtggc
    18541 gaagtcttgg tctgatgcgg ctggatcagc gacctgaaag aggcggtcat acaaccgaac
    18601 ctcaaccggc atggcatgct ctgctgagac ccaatgaatt accccttcta ctttgcggct
    18661 atcaggaggg ggcccgtttc gcgtttctgg gtcgtaggta cagtgaagct ctgttatctc
    18721 accgctcttc tcgtttttca cagtgttctt gtgcttgata acatatgaac ttctcagtct
    18781 gacctctttg ccaggggcga ggcgcttgta tttctttggc gggttttcgt cgaaatcttc
    18841 ctgctcgatg tagagaacct tagaaaaagg aaccctccgc gtacccattt caggtttttg
    18901 cggatggtta gcacattcaa gctcctctgt ctgtccgtct ggatagttgt cgatgataac
    18961 cttgagggga tgtaaaaccc cgagcactct tggtgcatgt tcattgagat catcacggac
    19021 gcagctttca agcaggccaa tatcgaccag gttgtcactc ttggccactc cgatcctggc
    19081 gcagaaatct ctgatggcct caggtgtata acctcgcctc ctcatacccg caacagtggg
    19141 cattcggggg tcatcccaac ctgaaacata gcccttttca acaagctcga tgagcctgcg
    19201 cttactaagc atggtatagc tcaagttaag acgggcgaat tcaatctggt ttggacggcc
    19261 atcagcgatc agctcgttga cgaaccaatc gtagagggga cgattattct caaactcaag
    19321 ggtgcagatg gaatgggtga tcttctcgat agcgtcagaa agacagtgag caaaatcgta
    19381 catagggtat atgacccaat tattaccagt ccggtaatgg ggggcgcgct taatgcggta
    19441 gatgacaggg tcacgcatgg tgacattcgg tgaagacata tcgatctttg ccctcagaac
    19501 atgggtgccg tcagcaaatt ctccggccct catacgtgcg aataaatcaa ggttttcatc
    19561 gactgagcgg tttctataag gactctcctt ccctggttct gtcagggtgc cacgtagcct
    19621 cctgatctcg tctgcgctca gactgtccac gtaagctttg ccgtccttga taagttgaat
    19681 ggcgaactgg taaagctgct cgaagtagtc agatgcataa aaaagacggt tttcccagtc
    19741 aaatccgagc cagtggacat cgttgataat agactcaaca tattcgagcg attcgcctgc
    19801 agggtcagtg tcatccatgc ggagattgca ggttcctcta tactgggcag caattccgaa
    19861 attgaggcac acagattttg cgtggcctat atggaggtat ccattcggct ctggaggaaa
    19921 acgggtagct acgcggccct tatgcttgtt agtcttgaaa tcgtcttcaa taatctcacg
    19981 aataaaatct gattggggtg ccatgtcctg gtttggcatt tgctcaaata atttccatta
    20041 tagatagaat tattctttac aagcacatat ggctgcgaac tggtaatgtc ttaccataag
    20101 ctttcggcgc ttgcaaggaa agagaacata agataggtac aactggctcc tcaatgccat
    20161 ctcatttctt tacaaatgct ttgctttata ggaaaatagt aacaatgagt acaaggctat
    20221 cttaccccct aacgatgagc accttatttt ttagtataat cctggggatt ctcataatcc
    20281 cctctccttg tcggggtgat gctgagaaga tattcaaagt gaatagtccg gctgttgtag
    20341 tagttgcttc aataaaccga aatcatgcaa taccacttgc tttatgtgac ataacccgat
    20401 aacatttcg
//
LOCUS       DAOOMC010000040        20276 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdus sp. isolate AMR_MDS_2685
            NODE_2618_length_20276_cov_6.952772, whole genome shotgun sequence.
ACCESSION   DAOOMC010000040 DAOOMC010000000
VERSION     DAOOMC010000040.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864760
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdus sp. (wastewater metagenome)
  ORGANISM  Syntrophorhabdus sp.
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae; Syntrophorhabdus.
REFERENCE   1  (bases 1 to 20276)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20276)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,729
            Genes (coding)                    :: 2,708
            CDSs (with protein)               :: 2,708
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 7 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20276
                     /organism="Syntrophorhabdus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2618_length_20276_cov_6.952772"
                     /isolate="AMR_MDS_2685"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2699756"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(110..1333)
                     /locus_tag="PK125_05635"
     CDS             complement(110..1333)
                     /locus_tag="PK125_05635"
                     /inference="COORDINATES: protein motif:HMM:NF002325.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HPB37622.1"
                     /translation="MQEALIQKAKKYIANTYNRYPIVATRGDGCWVWDLDGRRYLDFL
                     AGIAVCNLGHVRKEVLDDLIAQAKKLFHISNLFYMEPQIKAAEVIVDYSFGDKVFFCN
                     SGAEANEAAIKLARRYSWKKYGKERYEIIVMDNSFHGRTLCTLSATGQKKFWEGFDPL
                     VEGFTYVPFNNIGALQKSMSKKTCAVMLELIQAEGGIYVADKEYIKALRTLTKEKDVL
                     LILDEVQTGMGRTGKLFAYEHFDVTPDLMTLAKALGNGFPVGVLVGKDNIMEAFEPGS
                     HASTFGGNPLAAASVYATFTTMVGEGILKNCTEMGKYLHTHLLSLKKKYSFITDVRGK
                     GLIWGIELSIDGDMVQREFLKEGVILNCTKGTILRLVPPLIIGTEEIDIFHEIADRVF
                     ERFSKDTGSKADRLI"
     gene            complement(1335..2177)
                     /gene="argB"
                     /locus_tag="PK125_05640"
     CDS             complement(1335..2177)
                     /gene="argB"
                     /locus_tag="PK125_05640"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: protein motif:HMM:NF012899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HPB37623.1"
                     /translation="MSEKIKTLLEALPYIKRFAGSIFVVKYGGAAMEEENLKKEFAKD
                     MVLLKYVGINPVVVHGGGPKINKLLTDLNIPSNFIDGLRVTDEKTLEIAEMVLSGSVN
                     KEIVKNINDMGGKAIGLSGKDGRLLLAKKVKGKDIGLVGEIVGVDVSIIKDIIKHGYI
                     PVIAPIADGLDGKSYNINADTAAGSIAKALSAEKLILLTDVSGVLDKEENLISSLKKS
                     EVEILIKNKTVSGGMIPKVGCCVDAIEGGVEQTHIIDGRIPHAILLEVFTDSGIGTQI
                     TGGS"
     gene            complement(2189..3520)
                     /gene="hslU"
                     /locus_tag="PK125_05645"
     CDS             complement(2189..3520)
                     /gene="hslU"
                     /locus_tag="PK125_05645"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016448039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HPB37624.1"
                     /translation="MKTLTPREIMDELNRFIIGQKKAKKAVSIALRNRWRRQMVPKDL
                     RDEIAPKNIIMIGPTGVGKTEIARRLAKLANAPFLKIEATKFTEVGYVGRDVESMIRD
                     LTELSINMVRKEEQERVNEKAKVLAEERILDLLMPRRSRTVTPNEEEGEDTSREKLRT
                     RFRAGKFDETFVELEVPDRVLPIIEIFSGSGMEEMDMQIRDMFGNMLPKKTKKRKVKV
                     KEAYELLIQEESKKLIDSDKVTKDAIERVEQSGIIFLDELDKIASRNHTHGPDVSREG
                     VQRDILPIVEGTTVTTKYGMVKTDHILFIAAGAFHMSKPSDLIPELQGRFPIRVELDA
                     LTKDDFYRIITEPENALIKQYKALLATENIELNFNNDAIEEITDIAQRVNEMTENIGA
                     RRLYTVMEKLLDDISFTAPDMKEDTITITREYVRDKLGEFLQKDDLSRYIL"
     gene            complement(3522..4046)
                     /gene="hslV"
                     /locus_tag="PK125_05650"
     CDS             complement(3522..4046)
                     /gene="hslV"
                     /locus_tag="PK125_05650"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007302170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0051603 - proteolysis involved in protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HPB37625.1"
                     /translation="MEATTVLCVRHRGKVAIAGDGQVTIGSTVMKHTAKKVRRLYQDR
                     CLAGFAGATADAFTLFERFEKKLEQYNGNIMRASVELAKDWRTDRMLRRLEALLIVAD
                     AEHTLILSGTGDVVEPDDGVAAIGSGGTYAQAAAKALVKHTDLSPIDIVTESMRIASE
                     ICIYTNDRIVVEEL"
     gene            complement(4052..4939)
                     /locus_tag="PK125_05655"
     CDS             complement(4052..4939)
                     /locus_tag="PK125_05655"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HPB37626.1"
                     /translation="MDKNTLRHVSKEFYKYLEAEKGAAFNTKRAYKGDIEDFVDFVEK
                     SAFDVVDHQAIRAYIVNIYKDLKKSSLSRKVSSIKVFFKFMKRKGYIEENTALVIKNP
                     KIEKHLPKFYTIDEMFHFLDFLPKDGWLNIRNRAIFELMYSAGMRAQEALDINMNDIH
                     LEGMWVKVKGKGGKERILPFGEKAKHAIADYLPYRRQKGKASGKSPLFVIASGARLSY
                     RGLLKIMKKHQLKAHLFKDLALHGIRHSFATHMLDSGADLRSIQELLGHSKLSTTQKY
                     THVSMDKLMEIYDKSHPRR"
     gene            5336..6460
                     /locus_tag="PK125_05660"
     CDS             5336..6460
                     /locus_tag="PK125_05660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB37627.1"
                     /translation="MVREKITNDGVVSTYELETVYGPLIVESTVLLLKRIHELRALHK
                     MEVLQEGDVFTDAAKEAAMGTFYTAKNLVIDPVRTMSEVGNGIGRFFKRLNFGTANDD
                     PYRANALASALGQVAVKRELAYNFGVDPYSSYSPLQETLDSLAWTAVRGSLTVKTAFS
                     VLAFIPGGIAVVASLTSSADSLRSLVRDKTPLELQEINARKLAEMGVSDSIAKIFLGN
                     SSYNPSEQTLLVGELASMKSAKDRVMFIIAACYAKNEQMAVSMRAMAQIMSFYNEKVE
                     PIRGFIKAGNLPLLEKADGTVVAIMPYDYLAWTQRFANMERDVSDTLRRISGIREKEL
                     LTVGVMEPKTRNILEAKGWKIRERFAEATIQEIMAKNMKE"
     gene            6561..6848
                     /locus_tag="PK125_05665"
     CDS             6561..6848
                     /locus_tag="PK125_05665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB37628.1"
                     /translation="MPNDTQKGMYSCVPPTPGRRMLTFPDGTQVGVMGLNEIMADMYA
                     EGKQVNEGTAEEIVERLSEKNYITPSFRKHYCDVVLNEYRQYTKVRDNNER"
     gene            complement(7866..9695)
                     /locus_tag="PK125_05670"
     CDS             complement(7866..9695)
                     /locus_tag="PK125_05670"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HPB37629.1"
                     /translation="MKEEKTKEQLIQELVMAHQRIDELEKAEIQQRYRIIVDTADEGI
                     WVFDDQYITTFVNNRMIEILGYSQEEIMGKSLSFFLFDEDLPDFERKASLRRQGIAER
                     YERRIIRKDGSVLWTHVSATPIMDSEGRFQGSFAMFTDVTERKDAERALKKSEEKYRS
                     IFENAVEGMFQSTPEGQLITANPSMARIFGYTSQEDMVSQVNSIGLQLYAKEEYRRAF
                     QKLLKEHGFVGGFEAPFFRKDGSILWGTLNVRAVTDADKNILYYEGTLEDITPRKTAE
                     EELKKSEEKYRNIFENAVMGIYQVTTGGRFLSANPILASIHGYDSPEELINSVTDVSQ
                     LYVDPSRRTKLQRLMEEQGFVNGFDIIMRRKDGSLHWVSNTARSVRDEQGRVLYYEGT
                     IEDITSRKLAEESAKQWKQTLVGTLHALSQSIEIREPGIAGHHKSVSNLASAIARAMN
                     LGDDVAESTMMAGLVHDVGNMSVPSEVLSKPGRLSDLEYSFVKLHPRSGYDILKETGL
                     PYPVAEIVLQHHERMNGSGYPQNLKGKDTLLEAKILAVADVVEAITSNRPYRPALGVD
                     AALNEIRKNRGLLYDTRVVDACLELFKTKKFHFDKSGHPIVFG"
     gene            complement(9752..11323)
                     /locus_tag="PK125_05675"
     CDS             complement(9752..11323)
                     /locus_tag="PK125_05675"
                     /inference="COORDINATES: protein motif:HMM:NF012385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HPB37630.1"
                     /translation="MTQVYSSEANENQFFREMTLRICGSLEIEKALWHCFLYVRNIMP
                     ADELILTVYDPEAGVLEVVATADSNGGSASSDKITMPSSVRSHLEEPLKFPRVRMSEN
                     AYKDEIFAVVAERYKWVDSSIIVGRLIVENKFVGSLIVRARGIGRYSDDNASLWGLVN
                     EPAAVALANSRRYLELMNLKELLADDSKYFQNELRRGFSEEIVGAEFGLQGVMEQILK
                     VSPLSSPVLLFGETGTGKELVANAIHSFSPRHNGPLIKLNCGAIPETLIDSELFGHEK
                     GAFTGAVAQQRGRFERAHGGTIFLDEVSELPLNAQVRLLRVLQEKEIERVGGRQHIKV
                     DVRVVSATNKDLKKLVAEKAFRDDLFYRLCVFPIHIPPLRERKIDIPALVEFFLRKKA
                     KEIGLHFIPKLLPGTIEALLEYDWPGNVRELSNAIERAIIIQGGKPLSSEDIIGIPAQ
                     LGDKTRHESAQENLTLRDMEADHIRSVMKKAGGRVEGEQGAAALLGMNPGTLRHRMRK
                     LKIPFGRTAKKEYGS"
     gene            11667..12011
                     /locus_tag="PK125_05680"
     CDS             11667..12011
                     /locus_tag="PK125_05680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB37631.1"
                     /translation="MGKELKLKTAINKVRKSMEQSLVEIFGCVSFDGNATVKRNVVKD
                     RRQISRYFYLDRSPMEEMLVEIFGCIALDNKVILLDFIRDRKRSEREYDLPMVATESS
                     TEWLKRMHDRLS"
     gene            12225..13385
                     /locus_tag="PK125_05685"
     CDS             12225..13385
                     /locus_tag="PK125_05685"
                     /inference="COORDINATES: protein motif:HMM:NF017828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacyl-CoA dehydratase family protein"
                     /protein_id="HPB37632.1"
                     /translation="MTNFGNGSGLTVADKYYSQYGLRARELKGSGKKIIGYLSVLGPI
                     EILTAAGVVPFRLKGNVSEAITKGDAYMETIICPFVRNVFDAAIKGKFDFLEGMVLPH
                     QCDSIDRTDDVWANNLQLPYWHYLNVPHVTDDPSIEFMKDILRLLIRSLEKLTGKKIT
                     DDDIAQAVKAHNENRQLMRDLYVLRKSNPPMISGTEMMKVLVAAMSLPVDESSALIKS
                     VTEEVKARPMPANGKVPRIMLIGDQIDDVAIIDAIEGAGAHLVMDDLSIGSKMYWTDV
                     DATPDPIQGIAERYLRKLKIPTTFVGDGNTYQENLEARFGHMKQFIKEFKVDGAILFI
                     YKYCDPYGFDVPAMKSFIESGGTPVLYLEDEYSTSTLARVKTRIEAFLEMIA"
     gene            13406..14737
                     /locus_tag="PK125_05690"
     CDS             13406..14737
                     /locus_tag="PK125_05690"
                     /inference="COORDINATES: protein motif:HMM:NF017828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacyl-CoA dehydratase family protein"
                     /protein_id="HPB37633.1"
                     /translation="MAEERKPRTLATKAAASIPKFVRGNLAATLKAKEEGKKVADAYI
                     NDGQDEIIRAMDIVPAWGESFAGVCAAKREAEQYLRKAEADNFSRSLCTYATCTIGFD
                     MWREELGGEMPPKAPWGGMGRPDMIIGSAQQLCDPRFKWPQATQHYLQDVPVYVGGMY
                     YPQWDPNVDHHEQEEIYVKYARAELMELVRFCEKHTGKKMDWDRLSELVNLTEKTWDI
                     FIDAYELRRAIPTPMDTGDAMNTMVPLTFNLATQEAYDFYKALYDELTEKIKNKQGVA
                     ENEKYRIVWGAGLPSWFALGDFHYFNEKGAVFPVEVTYRNCEKIERLDLPPTNDPLER
                     IARRWVRMWTYWYDAARKRKGSIPKVERIISYIEEYKCDGVVFHSAFSCRSWHAGIVH
                     QAQVLKKVYGDIPILIMEGDIVDISSYNEADTHNRIDAFIEALDAYKKRSQ"
     gene            14885..15727
                     /locus_tag="PK125_05695"
     CDS             14885..15727
                     /locus_tag="PK125_05695"
                     /inference="COORDINATES: protein motif:HMM:TIGR00241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008047 - enzyme activator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydratase activase"
                     /protein_id="HPB37634.1"
                     /translation="MSPYFAGIDIGSTMTKVVIMGDEIVASIIGPTGPEHRKLANKVI
                     EEALIKAALSFEDLAYIVATGYGRINVPFADRQVTEITCHAKGIHHLLPKVKTIVDIG
                     GQDSKGIKISNGKVTGFVMNDKCAAGTGRFLEIIADSLGVPLVKLGEISLTADKAAEI
                     SSTCTVFAEHEVINKLSGGESVANLVAGIHESVATRVYALVNKLKIEPEVAITGGGAK
                     NIGLVKALEHKFGFPIVVPPEPLITGALGAALVGKEVYEKYASEGLVPTRSGHGLEEA
                     RLFS"
     gene            15815..16597
                     /locus_tag="PK125_05700"
     CDS             15815..16597
                     /locus_tag="PK125_05700"
                     /inference="COORDINATES: protein motif:HMM:TIGR00241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008047 - enzyme activator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydratase activase"
                     /protein_id="HPB37635.1"
                     /translation="MAFVLGIDVGSGYSKAVVCEDRSIRSFAVMPSGGDYKEIARTVA
                     TKALLEIGLSFNDVVYTVAAGYGAGMVDFADHSLTDISCHATGIHHLFPSVKTVIDVG
                     AQFSRAIRLDNDGRIANFILNEKCAGGSGKFLQTIARILHMNVNEIGDLSLTATNPVE
                     FTTACAVFAESEAVSRIAEGAAPADILAGLNKAMASKIITLVTRVSYAPDCALTGGGA
                     KNRGLVKAIQEELSTTVLVADEPQITAAFGAALVAFERIQAR"
     gene            complement(16759..17091)
                     /locus_tag="PK125_05705"
     CDS             complement(16759..17091)
                     /locus_tag="PK125_05705"
                     /inference="COORDINATES: protein motif:HMM:NF025715.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4404 family protein"
                     /protein_id="HPB37636.1"
                     /translation="MIEKTITSIENKVRDAKSIGEKDRTDLLKLLKELKTEVTELSKT
                     HAEHAESITGFTGVSTHEAMREQKNLQLMKLSLKGLSSSVDEMESSHPRLVAIVNKIA
                     QILANMGI"
     gene            17306..17881
                     /locus_tag="PK125_05710"
     CDS             17306..17881
                     /locus_tag="PK125_05710"
                     /inference="COORDINATES: protein motif:HMM:NF015325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HPB37637.1"
                     /translation="MKVVGFNGSARKDGNTAILIKYVFEELNKEGIDTELVQFAGKKV
                     AGCTACRKCFELKDKQCAVKDDIINECIGKMLGAEGIILGSPIYFADITPPIKSLIDR
                     AGYVAKANDDMFRRKVGAAVIAVRRAGAIHGFDSLNHFFFISQMIVPGSHYWNIGIGR
                     DKGEVEKDEEGKQIMKTLGQNMSWLLRKLYQ"
     gene            complement(17893..19047)
                     /gene="nrfD"
                     /locus_tag="PK125_05715"
     CDS             complement(17893..19047)
                     /gene="nrfD"
                     /locus_tag="PK125_05715"
                     /inference="COORDINATES: protein motif:HMM:NF015851.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysulfide reductase NrfD"
                     /protein_id="HPB37638.1"
                     /translation="MIEKSLKGDHIYWAWVAFLLVMIGIGFIAYMRQWDLGLGITGMG
                     RDISWGLYIANFTFLVGVAASGVMVVLPYYLHNFKVFARITIFGEFLAVAAVTMAMLF
                     IFVDLGQPARVFNIILYPSPHSVLFWDMIVLSLYLILNIIIGWRTLEAEHQEEAPPIW
                     LKPLVFISIPLAFSIHTVTAFIFSGLGARPFWLTALLAPRFLASAFASGPAILIIMCI
                     IMRRFAKFDIGQEATQKIALIITYALSIHIFFFLVEIFTVFYGGIPEHVHHFEYLLGR
                     GGQSILTISAWISLVLIWIAFLLMINPQTRKTEGTLIYALLAVIIAVWIEKGLSFIVP
                     GFIPSPLGEIARYSPTLAEIFITIGVWSVGILILTVLYKVTVSIKKGMVS"
     gene            complement(19054..19812)
                     /locus_tag="PK125_05720"
     CDS             complement(19054..19812)
                     /locus_tag="PK125_05720"
                     /inference="COORDINATES: protein motif:HMM:NF024247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HPB37639.1"
                     /translation="MMNRRDFLKLACFSVVGTPARSVFDRLTGKDIAGPTKEGQPKWA
                     MVVDLKMCASKPGCRDCVDACHNIHNVPAFNNPKDEVKWVWTDPYKNIFPEQSHRFVS
                     VEVQTRPVLILCNHCENPPCVKVCPTRATFKREDGIVMMDYHRCIGCRYCMAACPYGA
                     RSFNWKDPRPFIKKIKEDFPTRTKGVVEKCSFCEERLAKGLKPACVEACTTKALTFGD
                     LNEPNSQVRNLLASKPNIRRKAYLGTEPKVFYLE"
     gene            complement(19858..>20276)
                     /gene="dsrJ"
                     /locus_tag="PK125_05725"
     CDS             complement(19858..>20276)
                     /gene="dsrJ"
                     /locus_tag="PK125_05725"
                     /inference="COORDINATES: protein motif:HMM:NF038038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sulfate reduction electron transfer complex
                     DsrMKJOP subunit DsrJ"
                     /protein_id="HPB37640.1"
                     /translation="GHIMHDTAKVIAGIAVFLIVVLSPVWYNTVTGQASYVPQLKLPT
                     DEKYCVETAPYMRTNHIELLELWKKIVVRTGKRIYKATDGKTYTMSLAGTCMKCHSNK
                     ADFCDRCHNYTNVQPNCWECHVVPKGVKTEATEHAR"
BASE COUNT         5504 a         4600 c         4503 g         5669 t
ORIGIN      
        1 agttttttga ggaatgctgt ccgctcaagc aggtagctgc attcatcctt gctgatatcg
       61 agaagttttg taaagtcttt tttcaaagta aatcccctct cttacaatac tatataagtc
      121 tatccgcttt tgatccagta tctttcgaaa acctctcaaa aaccctgtca gcaatctcgt
      181 gaaatatgtc tatttcctct gtgccgataa taaggggggg aacaagtctc aaaattgtac
      241 ctttggtaca attcaatata accccttctt tcaaaaactc ccgctgaacc atgtcgccat
      301 ctatggaaag ctcaattccc cagatgagtc ctttccccct tacgtctgtt ataaatgaat
      361 atttcttttt aagtgagagg agatgtgtgt ggagatactt tcccatttca gtgcaatttt
      421 taagaatacc ttctcctacc attgtagtga acgtggcata aacagatgct gcagcgagtg
      481 ggtttccacc aaaggttgat gcatggctgc caggctcgaa tgcctccatt atattatcct
      541 tccccacaag tactccaaca ggaaatccat ttcccagggc cttcgcaaga gtcatgaggt
      601 caggcgttac gtcaaagtgc tcatacgcaa agagcttgcc tgttctgccc atgcctgttt
      661 gaacctcatc aagaatgagc aggacatctt tctctttcgt aagggtacga agcgccttaa
      721 tatattcctt gtccgcaaca tatataccgc cctcagcctg aatgagttcc agcatgacag
      781 cacaggtttt tttactcatt gacttctgca atgcccctat attattgaaa ggcacatagg
      841 taaagccctc aacaagtggg tcaaatccct cccagaactt cttttgtcct gttgctgaaa
      901 gagtgcagag ggtacgaccg tgaaatgagt tgtccatcac aataatctcg tagcgctcct
      961 taccgtattt tttccaggaa tatcttcttg caagctttat tgctgcttca tttgcctctg
     1021 ctccgctgtt gcaaaagaaa actttgtctc caaacgaata atccacgatg acttctgctg
     1081 ctttgatttg aggctccatg tagaacaggt ttgaaatatg aaagagtttt ttggcctggg
     1141 caattaagtc atcaaggact tccttacgca catgcccgag gttgcataca gcaatacctg
     1201 caagaaaatc caggtatctt ctgccgtcaa ggtcccatac ccagcaccca tcacccctcg
     1261 ttgcaacaat aggatacctg ttgtacgtgt ttgcaatgta cttttttgcc ttttgaatga
     1321 gcgcttcctg catctcaaga ccctcctgta atttgggttc ctatgcctga atcggtaaag
     1381 acctcaagaa gaatggcatg ggggattcta ccatctataa tgtgtgtttg ctccacaccg
     1441 ccctcaatgg cgtctacaca acaacctacc tttggtatca ttccacctga gacggtcttg
     1501 ttcttgatca ggatttcaac ttcggatttt tttaaagaag agatgaggtt ttcttcctta
     1561 tcaagaacac cgcttacgtc tgtaagaagt atcagtttct ctgctgataa tgccttggca
     1621 atgcttcctg ctgctgtgtc tgcatttatg ttgtaagatt taccgtcaag accatctgcg
     1681 atgggggcga tgacaggaat gtatccatgt ttgattatgt ccttgatgat tgagacatcc
     1741 acaccgacaa tctcaccgac aaggccgata tctttacctt tcaccttttt tgccaaaagg
     1801 agcctcccgt ccttacctga gagaccaatt gccttgccac ccatgtcatt gatgtttttg
     1861 acaatttcct tgttcactga acctgaaaga accatttcag ctatctcgag agttttctca
     1921 tccgttaccc ttagaccgtc tatgaaattt gatggaatat tcaggtcagt gagaagttta
     1981 ttgatttttg gaccaccccc atgtacgaca accggattga ttccaacata tttgagaagt
     2041 accatatctt ttgcaaactc tttttttaga ttctcctctt ccatggcagc accgccgtat
     2101 ttaactacaa atatggaccc ggcgaatcgt ttgatgtatg gtaatgcctc aagcagggtt
     2161 tttatctttt cgctcatgtt tccatccttt acaaaatgta tctgctcaga tcatcctttt
     2221 gcagaaactc tccaagttta tcccgcacgt attctctggt tatggtaata gtatcttcct
     2281 tcatatccgg cgcggtgaac gatatgtcat cgagtaattt ttccataaca gtataaagcc
     2341 ttcttgcacc aatgttttcc gtcatctcgt tcaccctctg cgcaatatca gtaatctctt
     2401 caatcgcatc gttgttgaaa ttaagctcta tgttttctgt tgccaacagt gccttgtact
     2461 gtttgatcaa cgcattctcc ggctcggtga tgatccggta aaaatcgtct ttcgtgaggg
     2521 catctaactc aacccttata gggaaacgcc cttgcagctc aggaatgagg tcggatggtt
     2581 ttgacatgtg aaatgcccct gcggcaataa atagtatatg atctgttttt accatgccgt
     2641 acttggttgt cacggtagtc ccctctacga tggggaggat atcacgctga accccttccc
     2701 gtgagacatc aggaccatgc gtatggtttc ggcttgctat cttatcgagt tcgtcgagaa
     2761 aaataatacc ggactgctca acccgttcaa tagcatcttt ggtgaccttg tcagagtcga
     2821 tcaatttttt ggactcctcc tgtatcagga gctcataggc ctctttcact ttgacctttc
     2881 ttttctttgt ttttttcggc aacatgttac caaacatatc gcgaatctgc atatccatct
     2941 cttccatacc agacccggaa aatatctcta tgatgggaag caccctatca ggcacctcga
     3001 gctcaacgaa ggtttcatcg aatttgcctg ctctgaaacg tgtcctgagt ttttccctcg
     3061 acgtgtcctc cccctcttct tcattgggtg ttactgtacg ggacctccga ggcatcagaa
     3121 gatcaagaat ccgttcctct gccagaactt tggctttttc attcaccctt tcctgctctt
     3181 ctttacgtac catatttatt gagagctccg taagatctct tatcatggac tccacgtccc
     3241 gtcccacata gcccacttct gtgaattttg tcgcttcaat cttaaggaag ggtgcattgg
     3301 caagcttagc aagccgccgt gcaatctctg ttttcccaac acctgtaggg ccgatcatta
     3361 ttatgttttt tggcgctatc tcatccctca ggtctttcgg caccatctgt cgtctccacc
     3421 tgtttcttaa agcgatggaa accgccttct tagctttttt ctggccgatg ataaaccggt
     3481 ttagctcgtc cataatctct ctcggagtga gtgtcttcat cttatagctc ctcaacaaca
     3541 attctgtcgt ttgtgtaaat gcaaatctcc gaggctatgc gcatggattc tgttacaata
     3601 tcaataggcg atagatcagt atgctttaca agtgcttttg ccgctgcctg tgcatacgta
     3661 ccaccggaac ctatggcagc aacgccgtca tcaggttcca ccacatctcc tgtccccgac
     3721 aggatgaggg tgtgctccgc gtctgccacg ataagcagtg cttcaagtct tcgaagcata
     3781 cggtctgttc tccagtcctt ggccagttcc acagaagcgc gcatgatgtt tccgttgtac
     3841 tgttcaagtt tcttctcaaa cctttcaaaa agagtaaagg cgtcggcggt tgcaccggca
     3901 aaccctgcga gacagcggtc ctggtaaagt cttcgtacct ttttcgccgt gtgtttcatc
     3961 acagtcgatc cgattgtgac ctgcccatct ccggcaatag caactttccc cctgtgccta
     4021 acacatagta ctgtcgtggc ttccattatt tttaccttct cggatgtgat ttgtcgtata
     4081 tctccatgag cttatccata gagacatggg tatatttttg ggtagtggag agttttgagt
     4141 ggccaaggag ttcctggata ctcctcaaat cagcgccact gtcgagcatg tgtgttgcga
     4201 aggaatgtcg tatgccatga agagcgagat ccttgaacag atgggctttc aattgatgtt
     4261 tcttcattat tttgagtaat ccccggtagg aaagccttgc tcctgacgca atcacaaaca
     4321 gagggctttt tcctgatgct ttacctttct gcctcctgta tggaagataa tccgcaatag
     4381 cgtgttttgc cttttcgcca aaagggagta tccgttcttt tcctcctttt cccttcacct
     4441 tcacccacat accctccagg tggatatcgt tcatgtttat gtcaagggct tcctgagccc
     4501 gcattcctgc tgaatacatg agctcaaaaa tcgctcggtt cctgatgttg agccaaccgt
     4561 cctttggcag gaagtcaaga aagtggaaca tctcatcgat ggtatagaat ttgggcaggt
     4621 gtttttcaat ctttgggttt tttatcacca atgccgtatt ttcctctatg tatcctttcc
     4681 gtttcatgaa cttaaaaaaa accttaatgg aagatacctt cctagaaagg gaagattttt
     4741 ttaaatcttt gtatatattt acaatgtatg ccctgatggc ttgatggtct acgacatcaa
     4801 aggcgctctt ctctacaaaa tcaacaaaat cctcaatatc acctttataa gccctctttg
     4861 tgttaaatgc tgctcctttc tctgcctcaa ggtatttgta aaattccttg gatacatgcc
     4921 tgagggtgtt tttatccatg gaagtattat tgtataattt tctgtcaaat aatcaaggaa
     4981 attcacataa gtgaacaaac gggttgactg acgggaaagg tggggagagg ggtgcattct
     5041 gcgttgggag ttgaacactc ggtgaaatga attaaagcag tacagagatt caaggatttt
     5101 tgctgcttgt agatataaac tggaaaagag acgcaacggt attttatatt actggtggcc
     5161 attaatgctc ggcaatttaa gaaattattt attcatattc tttttggtat tgttcccatg
     5221 tgtcattgga atgcagcccc ttcgtccagc cttttcacaa gattttcagc gcacgcctat
     5281 ttacctaagg gcctcagagg tcatcccgag ccaatggtta aaaggccaca attacatggt
     5341 aagggaaaaa attaccaatg acggcgttgt gagtacatat gagttagaaa cagtatacgg
     5401 accccttata gttgaaagca cggttctttt gttaaaacgt atacatgaac tgagagcttt
     5461 gcacaagatg gaggtgttgc aggaaggtga tgtctttact gacgcagcca aagaagccgc
     5521 tatgggtact ttctatacag cgaaaaatct ggttatagat cccgttagga ccatgtcaga
     5581 ggtcgggaac ggaataggcc gatttttcaa aaggctaaac ttcgggacag caaacgatga
     5641 tccttaccgt gcaaatgcct tggcatctgc cttgggacag gtggcagtga agcgagagct
     5701 tgcctataat tttggtgtag atccttattc gtcgtattcc ccactccaag aaacactcga
     5761 ttcacttgca tggacagcag ttcgcggaag ccttactgta aaaacagcct tttccgtatt
     5821 ggcatttatt ccaggcggaa tagctgtggt agcttctctg acgagtagcg ctgacagcct
     5881 gaggtctctc gttcgggaca agaccccatt agaactccag gaaataaatg cacggaaact
     5941 tgcagagatg ggcgtttctg actccatagc caagatattc ctgggcaact caagttataa
     6001 tccatccgaa caaacgctcc ttgtcgggga actggcaagc atgaagtctg caaaagacag
     6061 ggttatgttc attattgctg catgctatgc taaaaatgag caaatggccg tgtctatgag
     6121 agccatggcc caaataatga gtttctataa tgaaaaggta gagcccattc ggggtttcat
     6181 aaaggctggc aaccttcctc tccttgagaa ggctgatgga acagtggtag ccatcatgcc
     6241 ttatgattat ctggcatgga cccagagatt tgcaaacatg gagagagatg tatcagacac
     6301 actccgaaga atatctggta tcagagaaaa ggaactcctt actgtaggcg tcatggagcc
     6361 aaaaacgagg aatatccttg aagcaaaggg atggaagata agggaaaggt ttgccgaagc
     6421 aacaatacag gaaatcatgg caaaaaatat gaaagaatag gacaatggta gaacaaggta
     6481 acggcatcat gaagaataac aaagccaagc tgtgatcaga aaatcgaata aagtaatagg
     6541 ataacgactg gaggtagctt atgccaaatg atacacaaaa aggaatgtac tcctgtgtgc
     6601 ccccaacgcc aggccggcga atgctgactt ttcccgatgg tacgcaggtt ggtgttatgg
     6661 gtctgaacga gataatggct gatatgtacg ctgagggtaa gcaggtgaac gaggggactg
     6721 ctgaggagat tgttgagcga ctatcagaaa aaaactatat aacaccatcg ttccggaaac
     6781 attactgcga tgttgttctg aatgagtaca gacaatatac aaaggtgcga gataacaatg
     6841 aaagatagtg gactggtcag gatgtcaaca aaaaatagaa catttcaact tctgttgcct
     6901 ggcaagtatc ccttcttatc aaaccccaga taggtattga aatatacatg aatttcactc
     6961 caacaaaatt attcggcata aaaccaaata tctcactctt tgcgcggccg tcttgaatct
     7021 tcatgagcaa ccctgttcag aaatgaagat tggtatgcca taccaaatgg cactacgatt
     7081 cgtcccaatt tcagtgttac cgtatcttgg ggctgtgtct cctacaagag taggctgata
     7141 tgatcgatca gcagaccttc catatcatga gttttgacgt caggtgcgaa catacacgat
     7201 gaagaagatt gtctgtcccc cttgttcgtt gtttcactct atccgggatt ctgcatgaga
     7261 tacgcaagaa caactcagct caccaatttg tttcctcgat gaaggccagt ttgtatgggt
     7321 acttgaagcg gttcgggtgt gacgtgcaca gaaccctgaa gactggttca gcatttgcca
     7381 atctgaacag aagactccta tgggcttacc ggtctctttt gaacagatta tgaaacgaac
     7441 actctatcta ttacttacgc gccttttaga caagcactgt gcattattaa tacacagtga
     7501 caccgaacaa ccatcccggg ttcggtttaa aaaagagagg cttaggaaat gaaagctgca
     7561 atcaggacat tcgtatcaaa gaccgccttt acgttatagc actgaaaacg tccttatggg
     7621 ccacaattcc ggctttttcg tcttctaaaa atacctgatt ttatctgatt aaatcgggca
     7681 tcccagcaaa aaagaccagt agtttccttg atgatcccac ttttgtttct tcctgtcagc
     7741 cttgtaaagc ggccaagctc ttctgctgtg ctttgtggaa ggttaatcga cattacaaat
     7801 agctacaaat gctaaaccgc caacaaaatt attattcctg tggagccatg gagcctgccg
     7861 atgagtcaac caaacactat aggatgtcct gatttatcaa aatgaaactt ctttgttttg
     7921 aaaagttcaa gacaggcgtc tacgactcgg gtatcataga gtaaacctct gttcttccgt
     7981 atctcattaa gggctgcatc aacacccagg gctggcctgt aagggcggtt agaggttata
     8041 gcttccacca catctgctac tgcaagaatt ttcgcttcga gcagtgtatc cttacctttg
     8101 agattttggg gataccctga gccattcatt ctctcgtggt gctgaagcac aatctctgcc
     8161 acaggataag gcaaaccggt ttctttgaga atgtcgtaac ccgagcgagg atgaagcttg
     8221 acaaaactgt actccaggtc gctcaaccta cctggtttgc tcagaacctc tgagggaaca
     8281 gacatattgc cgacgtcatg aacaagacct gccatcatgg tactctctgc aacgtcatcc
     8341 cccagattca tagccctggc aatcgcactg gcgaggtttg acacactttt gtggtggcca
     8401 gctatgccag gttctctgat ttcaatcgat tgtgaaaggg catgaagggt gcccactagg
     8461 gtctgtttcc attgtttcgc actttcttct gcaagttttc gtgaggtgat atcctcgata
     8521 gttccctcat agtagagaac cctgccctgt tcatcacgga ctgagcgagc cgtgttggag
     8581 acccagtgca gactcccatc ttttcgtcgc atgataatat caaaaccatt tacaaaaccc
     8641 tgttcctcca tcagtctctg gagttttgtg cgacggcttg gatcaacata aagctgactg
     8701 acatcagtta ctgagttgat caactcttca ggggaatcgt atccatggat actcgcgaga
     8761 atggggtttg cgctgagaaa tcgccctcca gtggtaacct ggtaaatacc catgaccgcg
     8821 ttctcaaaaa tattgcggta tttctcttct gattttttta attcttcctc agctgttttg
     8881 cgaggtgtta tatcctcaag cgtgccttca tagtaaagta tattcttgtc ggcatcggtc
     8941 actgcccgga cattaagcgt tccccataga attgacccgt ctttccggaa aaacggtgcc
     9001 tcaaatcctc ctacgaaacc gtgttctttc agaagcttct gaaaggccct tctgtattct
     9061 tccttggcat aaagctgcag acctatgcta ttcacctggg aaaccatatc ttcctgtgaa
     9121 gtatagccaa aaatgcgtgc catggatggg ttggcggtta tcagttgccc ctcgggagtg
     9181 ctctgaaaca ttccttcaac agcattctca aagatgctgc gatatttttc ctcactcttt
     9241 tttagtgctc tttctgcatc cttacgctcg gtaacgtcag tgaacattgc aaaggaccct
     9301 tgaaaacgac cctcgctatc catgatgggc gttgctgata catgggtcca tagaacgctc
     9361 ccgtcttttc gtataatcct gcgctcatat cgttcagcga taccctgcct tcgcagggaa
     9421 gcctttcgtt caaaatcagg aaggtcctcg tcaaatagga aaaaagaaag gcttttcccc
     9481 atgatttcct cttggctata acccagtatc tcaatcatgc ggttattgac aaaggtcgtg
     9541 atatattgat catcaaaaac ccaaatacct tcgtctgcag tatctactat aatgcgatac
     9601 cgctgctgga tttctgcctt ctcaagttca tcgatccgtt gatgtgccat caccagttcc
     9661 tggatcaatt gctcctttgt cttttcttcc ttcattatat tttgtccttt gcccctactc
     9721 tcctttttaa tcccttaatt tctccaatct tttaacttcc gtattccttt tttgccgtgc
     9781 gaccaaaggg gatttttaat tttcgcatcc tatgccgtaa agttccagga ttcattccta
     9841 aaagcgctgc agccccctgc tctccctcaa cccttccacc cgcctttttc ataaccgacc
     9901 tgatgtgatc ggcctccata tcgcgcaaag taaggttttc ctgggccgat tcgtgtctgg
     9961 ttttgtcgcc cagctgtgct ggaataccaa taatgtcttc agagctcagc ggcttgcctc
    10021 cctggataat gatggctcgt tcgatggcat tgctcaattc tctcacgtta cccggccagt
    10081 cgtattctaa gagcgcctct atggttcctg gtaaaagttt tggaataaaa tgaagtccga
    10141 tttcctttgc ttttttccgg aggaagaact ccacgagggc tggtatatct atcttccttt
    10201 ctctgagagg cggaatgtgg attggaaaaa cacacaaacg gtagaaaaga tcatctctga
    10261 atgctttttc agcaacaagc tttttcagat ctttgtttgt tgcagaaaca acccgcacgt
    10321 caacctttat atgctgcctg cctccgacac gctcgatctc tttttcctga aggacccgca
    10381 ataagcgaac ctgggcgttt aggggaagct cactcacctc atcgagaaaa attgttccgc
    10441 cgtgggctcg ttcaaacctt cccctctgtt gcgctacggc tccagtaaat gcccccttct
    10501 cgtggccaaa aagctcgctg tcaatgagcg tctcaggtat tgccccacag tttaacttga
    10561 ttagaggccc attgtgtcgt ggcgagaaac tatggattgc attcgcaaca agttctttgc
    10621 cagtgcccgt ttctccaaag agcagcacag gacttgatag tggggaaacc ttgagaatct
    10681 gttccatgac tccttgcaga ccaaactctg cgccaactat ttcttcgctg aaaccgcggc
    10741 gcaattcgtt ctgaaagtac ttgctgtcat ctgccaggag ttctttaagg ttcatcaact
    10801 ccaggtagcg cctgctgttg gcaagagcga cggcagccgg ctcattgaca aggccccaca
    10861 gtgacgcatt atcatcagaa tatcttccta tccctctggc acgaacgatc aaagagccta
    10921 caaatttgtt ttcaacaatg agcctaccaa caataataga agaatcaacc cacttgtaac
    10981 gctcagcaac aacagcaaat atttcatctt tgtatgcatt ttcagacatt cgtacccttg
    11041 ggaactttag aggttcctcc aaatggcttc ttacagacga aggcatggta attttatcgg
    11101 agctggcaga accgccattg ctgtccgctg tggccacaac ctcaagcact cccgcctctg
    11161 ggtcatacac ggtaagaatc aactcatcag caggcattat gttgcgaaca tagagaaaac
    11221 aatgccaaag cgccttttca atctcgaggc tcccgcaaat acgcaatgtc atttctctga
    11281 aaaattgatt ttcattggct tctgatgaat aaacctgagt catatctgtc atattttaca
    11341 gtttatataa tttatggaga agtatcaatg tatttatata aaataagata ctatgattat
    11401 gctaacagag cgaatactca gtgctgccag atatgatata ggaaagagtt tgaaaaaatg
    11461 cttaggaatg ttgtttacaa atccgtggta tgtgtcagtt aatcatgtat ctgacatata
    11521 ttacagctca aatctgatat atatatcagt aatgagctgg gcgttttttt aatatgctaa
    11581 ttttattgaa catattatat ggcacatatc ttgctttagt ataatagtaa atgagatagc
    11641 agtcagataa gacaaaggag ggtaacatgg gaaaagaact taaactgaaa actgcaataa
    11701 acaaagttag aaaatctatg gaacagtcat tggttgaaat atttggctgt gtttcttttg
    11761 atgggaatgc aacggtcaag agaaacgttg ttaaagacag aaggcagatc agccgctatt
    11821 tttaccttga cagaagcccc atggaggaaa tgcttgtcga aatattcgga tgcattgccc
    11881 tcgataataa ggtaattctg ctcgacttta tcagagacag aaagagaagt gaaagggaat
    11941 atgacctccc tatggttgca acagaatcat caacagagtg gcttaaacgt atgcacgatc
    12001 gactttcatg aaaagcgtga tatttcttgc ggcatcgagt tctacttcag ctaatgaaga
    12061 aaaaagggag gctaacaagt ctcccttttt tcttccgtgt aacactgttt aacttttaga
    12121 tatggaaaaa atgagcgcga ttaagttata ttattcacaa gcagcaggga aacaacttat
    12181 taatatcccg tcatcttgct acgagataga aaaggagttt aacaatgaca aattttggaa
    12241 acggtagtgg tttaacagta gctgacaaat attacagtca atatggattg agggcgcggg
    12301 agctgaaagg gtcaggtaaa aagatcatcg gctatctctc ggttcttggt ccgattgaaa
    12361 tcttgacagc ggcgggggtt gtgccgttca ggttgaaggg caacgtatct gaagcaatta
    12421 ccaaagggga tgcttacatg gagacaatta tttgcccctt tgttcgtaat gtgttcgacg
    12481 cagcgatcaa gggaaagttt gattttcttg aaggtatggt gttgccccac cagtgtgaca
    12541 gcattgacag gacagatgac gtgtgggcta ataaccttca attaccgtat tggcactatc
    12601 tgaatgttcc ccatgttacc gacgatccat caattgagtt tatgaaggat atcctgcgcc
    12661 tcctcatcag gagtctggag aagctcactg gaaaaaagat cactgatgac gatattgctc
    12721 aggcagtcaa ggctcataac gaaaaccggc agcttatgag ggatctgtat gtgttacgta
    12781 aatcaaatcc ccccatgatc tcagggacag agatgatgaa ggttcttgtg gcagccatga
    12841 gccttcctgt ggacgaatcg agcgccctga taaaaagtgt cacggaagag gtaaaagcaa
    12901 ggccaatgcc tgccaacggg aaggtaccca gaattatgct catcggtgac cagattgacg
    12961 acgttgccat tattgatgct attgaggggg caggggccca cctggtcatg gatgatctat
    13021 ccatagggag caagatgtac tggaccgatg tcgatgcaac ccctgacccg atccagggta
    13081 tcgcggaacg ttatttaaga aagcttaaaa tacccactac gttcgttgga gacggtaaca
    13141 catatcaaga gaacctggaa gcacgattcg gtcacatgaa acaatttata aaggaattta
    13201 aggtcgatgg agccattctc ttcatttata aatattgcga tccctacggt tttgatgttc
    13261 cggcaatgaa gagcttcatc gaatcagggg gaaccccagt tttatacctg gaggatgagt
    13321 attctacctc cacccttgcc cgggtgaaga ccaggatcga ggcattcctt gaaatgatcg
    13381 cataacgccg caggaggaaa attacatggc tgaagaaaga aaaccaagaa cattagcaac
    13441 aaaagcagca gcaagcatac caaaattcgt tcgtggcaac cttgcggcca ctctgaaggc
    13501 aaaagaggag ggtaagaagg tagctgacgc atatattaac gatggtcagg atgagattat
    13561 ccgggccatg gatattgtac cagcttgggg cgaaagcttt gccggagtct gtgcagcaaa
    13621 gcgtgaagca gagcaatatc tgcgaaaggc agaagccgat aacttttcca ggtcactctg
    13681 cacgtacgca acatgtacca taggttttga tatgtggcga gaagaacttg gcggtgagat
    13741 gccccccaag gctccatggg gcggtatggg aaggccggac atgatcatcg gcagtgcaca
    13801 gcaattgtgt gacccccggt ttaaatggcc tcaggcaacg cagcactatc ttcaggatgt
    13861 ccccgtctac gtgggaggta tgtattatcc gcaatgggac ccgaatgtgg accaccatga
    13921 acaggaagag atctatgtga aatatgcaag ggcagagctc atggagctag ttcgcttctg
    13981 tgaaaagcat accggaaaaa agatggattg ggatagactt tcagagctgg tgaatctaac
    14041 ggaaaagacg tgggatatat tcatcgatgc ttatgagctg cgcagggcaa ttccaactcc
    14101 catggacacg ggcgacgcaa tgaataccat ggtgccgctc acgtttaatc tcgcaaccca
    14161 ggaagcctat gacttctaca aggctctcta cgacgaactt acagaaaaga ttaaaaacaa
    14221 acagggcgta gctgagaatg aaaaatacag aattgtctgg ggcgcaggac ttccttcgtg
    14281 gtttgctctc ggtgatttcc actatttcaa tgagaagggt gccgtattcc cagtggaggt
    14341 cacctaccgc aattgtgaga aaatagaacg ccttgatctt cctccgacaa atgatccatt
    14401 agagcggatt gcccgaagat gggtaagaat gtggacctat tggtatgatg cagcgcgcaa
    14461 acgtaaaggc tctataccaa aagtcgagcg tattattagt tatattgagg aatataaatg
    14521 cgatggagtg gtttttcact ccgccttttc atgccggtcg tggcacgccg ggattgtgca
    14581 ccaggcacag gttctgaaaa aggtttatgg tgatatacct atccttatca tggagggaga
    14641 tattgttgac atcagttctt acaatgaggc agacacccac aacaggatcg acgcatttat
    14701 agaagcgctc gatgcctata agaaaagaag tcagtaaacc taattggtga cgcgtgatgg
    14761 gcttcaacac aaacagaaca ggcattgcaa tgaaggggca tcgccacaag ccaaacaggg
    14821 aatactgtcc ctatcactcc ttcccgtcac gcgttacaga tcatgtatca cagggtctgt
    14881 tgccatgagc ccatattttg ccggcataga catcggttcg acgatgacca aggtggttat
    14941 aatgggcgac gagatcgtcg cctcgatcat aggacctacg ggacctgaac acagaaagct
    15001 tgccaacaaa gttatagaag aggcgctcat caaagcagcc ctcagttttg aagatcttgc
    15061 ctatatagtt gctacaggtt atggacggat caatgtcccc tttgctgaca gacaggtgac
    15121 tgaaataaca tgtcatgcca aaggcatcca ccaccttctc cccaaagtga aaacgattgt
    15181 tgatattggt ggccaagaca gtaaagggat caagataagt aatggtaaag tgacaggctt
    15241 tgttatgaac gataagtgcg ctgctggcac aggaagattc ctcgaaatca ttgctgactc
    15301 acttggcgtt ccccttgtaa aactcgggga gatatccctt acagctgaca aagctgcgga
    15361 aatcagcagc acatgcacag tttttgccga acatgaggtg attaataaac tttccggcgg
    15421 agaatcagtt gcgaatctgg tcgccggcat tcatgaatca gtagccacaa gggtctatgc
    15481 gctggtgaac aagctgaaga ttgaaccaga agttgcaatc accggtggtg gtgcaaaaaa
    15541 tattggtttg gttaaggccc tggaacataa gtttggtttt ccaatcgtag ttcctcctga
    15601 acctcttatc actggtgccc ttggtgcggc ccttgtgggg aaggaagtat atgaaaaata
    15661 cgcatccgaa ggcctggtac ccacaagaag cgggcatggc ttggaagaag cgcggttgtt
    15721 ttcatgaaca attacagcgt taaactgtaa gtaagaaaga aaggtttttc attacgttct
    15781 tcgatatgcg aaaaacactt gacggatttt atttatggca tttgttcttg gtatagatgt
    15841 aggttccggt tattccaaag cggttgtgtg cgaagataga tccatccgct cctttgctgt
    15901 catgccttcc ggaggggact ataaggaaat tgcacggact gttgcgacga aagcgctttt
    15961 ggagataggg ctttccttca atgatgttgt atataccgtt gcagcaggat atggcgccgg
    16021 tatggtcgat tttgcagatc attctttaac agatatttcc tgtcatgcca caggcattca
    16081 tcatcttttt ccatctgtta agactgttat agatgtgggt gcacaattca gcagggccat
    16141 tcgccttgat aacgacggaa ggattgcaaa ctttatcctc aatgaaaagt gtgctggtgg
    16201 aagcggaaaa ttcctacaaa ccatcgcccg tatactccat atgaatgtga acgagattgg
    16261 tgacctctcc ctcactgcca ccaatccggt tgaattcacg acagcatgtg cagtgtttgc
    16321 cgagtccgaa gcggtctccc gtatcgccga aggtgctgcc cctgcagata ttcttgccgg
    16381 gctgaataaa gccatggcat caaaaattat cacccttgtt acacgtgtaa gttatgctcc
    16441 tgattgtgct ctcactggtg gaggggcaaa gaatagaggt ttagttaagg ccattcaaga
    16501 ggagttgtca accactgtcc ttgttgcaga tgaaccccag atcactgcag ctttcggagc
    16561 agctcttgtt gctttcgagc ggattcaggc aagatagtac ttcataatta aatcccacgc
    16621 tctgtttgtt atataattta ttcgacgatc ttatacccca aaaacgtctt ttctctttgt
    16681 cgagtttgtc ccagagtttt accagttttt tatccattca ccatgaatct acccagcgac
    16741 tacagaacag atgtcggttc agattcccat attagccaga atctgggcga ttttattgac
    16801 tatggccaca agccggggat gtgaactttc catctcatca acagaggagg aaaggccttt
    16861 cagagaaagc ttcatgagtt gtaggttttt ctgttcccgc atagcttcgt gcgttgaaac
    16921 cccggtaaag ccggtaatgc tttcggcgtg ttcggcatgg gtttttgaaa gctcggtcac
    16981 ttcagttttg agctctttga ggagctttag aaggtcggtc ctgtctttct cacctattga
    17041 ctttgcgtca cgtaccttgt tttctatact tgttatagtc ttttcaatca tgcctgcctc
    17101 ctctgacgtg agggtataat ccaagctcta cccgtaaaaa tcatagcaga agttactgga
    17161 acgcgcaagg cgcccggtct ttgcagtgct cactgttggt cttggggcat atggttgaca
    17221 ttcaaaaaat cctttgatag tatcgtagaa aaaaccgtct atcgaccacg agggttcggc
    17281 acggaaagaa aaggggggag gaacaatgaa agtggtggga ttcaacggaa gcgccaggaa
    17341 agatggaaat actgcaattc ttattaaata tgtatttgaa gagctgaaca aagagggaat
    17401 tgacactgaa ttggttcagt ttgcaggaaa aaaggtggca ggctgcacag catgccggaa
    17461 gtgtttcgaa ttaaaagata agcagtgcgc tgtcaaagac gatataataa acgaatgtat
    17521 tggaaaaatg cttggcgcag aggggattat actcggatcc cccatatatt ttgccgatat
    17581 tacaccacct atcaagtccc tcattgatcg tgcaggatat gtggcaaagg caaacgatga
    17641 tatgttccgg aggaaggtcg gggctgcggt gatagccgtg cgaagggcag gagcgatcca
    17701 cggatttgat tctctcaacc atttcttttt tataagccag atgattgtgc cgggatctca
    17761 ctactggaac attggcattg gtcgagataa aggtgaggtt gagaaggacg aggaaggtaa
    17821 acagattatg aagacactgg gacagaatat gtcgtggctt ctaagaaaac tgtaccaata
    17881 ggcacgtttg gatcaagaaa ccatcccctt cttaatagaa actgtgacct tgtaaagaac
    17941 cgtaagaatc aaaatgccaa ctgaccatac acctattgtt ataaaaatct cggctaaggt
    18001 cggagagtat cgggctatct ctccaagagg tgaagggata aacccgggta caataaaact
    18061 gaggcctttc tcgatccaca cagcgataat tacggcaagc agtgcataaa tcagcgtgcc
    18121 ttcggttttc ctcgtctgtg gattgatcat aagaagaaaa gcaatccata tcagaaccaa
    18181 agagatccat gcagaaatag tgagtatgga ctgtcctcca cgacccaaga ggtattcaaa
    18241 gtgatgcacg tgctcaggaa taccgccata aaatactgta aatatctcaa ccagaaaaaa
    18301 gaagatatgt attgataagg cgtaggtaat aatcaaggct attttttgag ttgcctcctg
    18361 cccgatgtcg aattttgcaa accttctcat aataatacac atgataatta gaatggcagg
    18421 accagaggca aatgcagagg ccagaaaacg aggggcaagg agcgccgtga gccagaaagg
    18481 tctggctcca aggcctgaga agataaaggc tgttaccgta tggatgctga atgcaagggg
    18541 tatagaaata aaaacaagtg gtttcaacca aatcggtgga gcctcctcct gatgctctgc
    18601 ctcaagggta cgccaaccaa tgatgatgtt aaggatcaga taaaggctga gtacaatcat
    18661 atcccagaac aagacggaat gtggagacgg gtaaagaata atattaaaga cacgggctgg
    18721 ctgaccgagg tcaacgaaaa taaacaacat agccatagtt acggcagcaa cagcaaggaa
    18781 ttcaccaaag attgtgatcc ttgcaaaaac cttgaagttg tgcaggtaat atggaagaac
    18841 taccatgacc ccagatgctg caacacccac caaaaaagtg aaatttgcaa tgtagagtcc
    18901 ccatgagatg tcccttccca taccggttat gccgaggcca aggtcccatt gcctcatata
    18961 tgcgataaac ccgataccaa tcattacgag aagaaacgcc acccatgccc aatagatatg
    19021 atctcctttt aaggactttt caatcataga gcctcattct aaataaaata cctttggttc
    19081 tgttccaaga taagcttttc ttctgatatt cggtttagat gcgagtaaat ttctgacctg
    19141 agaattgggt tcgttaagat ctccaaatgt gagtgctttc gttgtacaag cctctacgca
    19201 tgcaggcttc aggccttttg cgagtctttc ctcgcagaaa ctgcattttt cgacgacccc
    19261 ttttgtacgt gttgggaaat cctctttaat ttttttaatg aagggacgtg gatctttcca
    19321 gttgaaactt cgcgcaccat acgggcaagc tgccatgcag tacctgcacc cgatacagcg
    19381 atgatagtcc atcataacga tgccatcctc cctcttgaat gttgctctgg ttggacagac
    19441 cttaacgcat ggagggtttt cacagtgatt gcacaggatt agcacaggtc ttgtttgaac
    19501 ttccacagat acaaaccgat gggattgctc aggaaatatg ttcttatagg ggtctgtcca
    19561 gacccactta acctcatcct ttgggttgtt gaaggctggt acattatgta tgttgtgaca
    19621 ggcatcaaca cagtcacggc aacctggttt tgaagcgcac attttgagat caactaccat
    19681 ggcccatttt ggctggcctt ccttggtcgg tccagcaatg tcttttccag tgagtctatc
    19741 aaaaactgac ctggctggcg taccaaccac agagaaacat gccagtttga gaaaatcgcg
    19801 tctattcatc atttttcaaa atactgtgat agctgcacgc agaccacgtc ccacaaccta
    19861 tcgagcatgt tctgtagcct cagttttaac tcctttgggc accacgtgac actcccagca
    19921 attcggctgc acatttgtgt agttatggca cctatcacag aagtcagctt tattagagtg
    19981 gcatttcatg catgttcctg ccaggctcat ggtatatgtt tttccatcgg ttgctttata
    20041 gattcgctta cctgttctta caacaatttt tttccacagc tccagaagtt caatgtggtt
    20101 tgttcgcata tatggagccg tctcgacgca atatttttca tcggttggta attttaactg
    20161 ggggacatag cttgcctgac ctgtaacagt gttataccag actggactca gcacaacaat
    20221 aaggaagact gcaatcccag caataacttt agcagtgtca tgcattatat gtccct
//
LOCUS       DAOOMC010000041        20159 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdus sp. isolate AMR_MDS_2685
            NODE_2648_length_20159_cov_6.460157, whole genome shotgun sequence.
ACCESSION   DAOOMC010000041 DAOOMC010000000
VERSION     DAOOMC010000041.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864760
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdus sp. (wastewater metagenome)
  ORGANISM  Syntrophorhabdus sp.
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae; Syntrophorhabdus.
REFERENCE   1  (bases 1 to 20159)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20159)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,729
            Genes (coding)                    :: 2,708
            CDSs (with protein)               :: 2,708
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 7 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20159
                     /organism="Syntrophorhabdus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2648_length_20159_cov_6.460157"
                     /isolate="AMR_MDS_2685"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2699756"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            615..1559
                     /locus_tag="PK125_05730"
     CDS             615..1559
                     /locus_tag="PK125_05730"
                     /inference="COORDINATES: protein motif:HMM:NF016356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 and DUF1722 domain-containing protein"
                     /protein_id="HPB37641.1"
                     /translation="MDKIKIGISRCLLGENVRYDGGHKHDRYLTDILGKYFEYTPVCP
                     EVEYGLPIPREPLRLVGKPEDARLVTLNTGIDHTDGMLEWAKGKLKELEEKDLCGFVF
                     KSKSPSSGMQSVKVYGPSGVPVRKGVGIFAGAFTKRFPNLPVEEDERLYNTVLRENFM
                     ERVFVYKRWQDMLREGKNIKNLRDFHIAHKFLVMSHSPRHYRNLEKLMVDVKSYKIDV
                     YEEYVKTLMEGLRLIATVKKQVNVLRRIMGYLNRYLTTDEKQEVQEVIHNFHQQLVPL
                     ITPITLLTHYIRKYGISHLERQHYLYPHHVELMLRNHI"
     gene            1766..2074
                     /locus_tag="PK125_05735"
     CDS             1766..2074
                     /locus_tag="PK125_05735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB37642.1"
                     /translation="MGVYSDIYEFAARAGAFEGYVYQREGLTAESLERWVDHLVEGYN
                     AVAPDIRKEFQSLCDGTIGRAIQSLIITLGEHHEIIRKLRGLTTGKLPSSPDDFSRKR
                     "
     gene            complement(2060..2608)
                     /locus_tag="PK125_05740"
     CDS             complement(2060..2608)
                     /locus_tag="PK125_05740"
                     /inference="COORDINATES: protein motif:HMM:NF013607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB37643.1"
                     /translation="MTKKLGSNMVEMKPIRLVCFVCEWNEGRSVHLEMSVRLKLKRLG
                     SKIIVMSAGFSQADTVNPLRKAFLLGLGVPMSEIDAHFSTIFNEKHARADLILVAELP
                     MKGKLVQQWPGLTGRVMTVKGFVKGMNPSNENISEEEAMMEDAGGKDPDKKLMLYVKH
                     EQLAAQVAQRLVELEAGRSSFP"
     gene            2811..3992
                     /locus_tag="PK125_05745"
     CDS             2811..3992
                     /locus_tag="PK125_05745"
                     /inference="COORDINATES: protein motif:HMM:TIGR02486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reductive dehalogenase"
                     /protein_id="HPB37644.1"
                     /translation="MSGYLNRKWETFPVHKLKRVPNPTTLIHSDMVQRVREREAGFCK
                     SAAGDYGPKLETETRRFVPKHPASGALSWMRNNMKPFLDGITSAHQAPIPDDPASLAR
                     HIKEMAYFLRADTVGICELPPYAVYSHKFDFMKPEAAEVPIELNHKYAIAVLIDQDYR
                     TSHATSGSDWISNAMSMLAYTTSGFIAIILAEYIRRLGYPAKAHYAPFYDLVVPPILL
                     LAGLGEMCRIGDIVLNPFLGPRFKAAVVTTDMPLLSDKPIDFGLQDFCSKCKKCAREC
                     PSGAISDGGKEMYNGYEKWPMDKKKCTGMRVGNQRGSGCGTCIKVCPWNKPYTPFHRS
                     VGWAMRNSSIARSFAIWGDDLLGYGKPDYSQKWWFDLEDVNGDGVLSIPANKKSEFEG
                     R"
     gene            3994..4815
                     /locus_tag="PK125_05750"
     CDS             3994..4815
                     /locus_tag="PK125_05750"
                     /inference="COORDINATES: protein motif:HMM:NF019349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HPB37645.1"
                     /translation="MKQQPIHQKKEGKSTVTPTFQPLDKRRYFEQVAQLIRTKIFDEG
                     LVEGFRLPSEVDIANELRVSRSVVREALRILDVMGYIHAKKGPQGGIFVSSHYHKPVT
                     ESFRSLAENGQITVDHLFDVRLQIEPFIIEEATVHAKDEDIKKIALLFQDASRAMDDA
                     AKLKRANIDFHLLLAEASGNPVLSILMKSLTDILMEVAYTFLDLDFERQLFEIHKEIF
                     HAIKSRKTGEVSRLINADILFVKENLKNYVERGDIRNAGEQALKETPAFKRKKGG"
     gene            4817..5845
                     /locus_tag="PK125_05755"
     CDS             4817..5845
                     /locus_tag="PK125_05755"
                     /inference="COORDINATES: protein motif:HMM:NF015445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HPB37646.1"
                     /translation="MKRHELLIGFGLLMFLLGLFLYCPAVQAQEKVITMNYAHFMPIM
                     TKQAQLGEQWCREVEKRTNNRVKITFYPGGTLATAPLVYDAVVKGIADVGWSFLSYTR
                     GRFPLSEVIDLPLGYKSGYAATKMTNEYYKKYKPKELDDVKVMYLHAHGPGLLSTKKA
                     VYKLSDLKGMKIRSTGLSAKIVQALGGATVGMSIAEAYDALRTGVVDGILIPVEALEQ
                     WKLGELTQFTTESYSSAYTTAGFCVMNKAKWNSLPADIQNIIEKINEEWIEKTGLLWD
                     QMDKDGKAFALSKGIKFIPLSQEESALWAAKVKPIVDEYVQTMKSKNLPGEEVLKFCQ
                     DYLTKTQK"
     gene            5973..6647
                     /locus_tag="PK125_05760"
     CDS             5973..6647
                     /locus_tag="PK125_05760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB37647.1"
                     /translation="MVEFIRFSLSRLSRLIFSLFTFQFVFFTSFPSVLVRPAFPVNFG
                     ASDLQFCYNGTYNKALSRGFSMISKGWHRLVSIISVVGVIGLLLAFPLLSRAVEVDSE
                     LMEHFKTNETGSYVIYFRAKASFAGAPNSSWKEQNEFINRALQDNANRSQARVVSYLF
                     HHRVQYRSLWRDNTVIVEKSNKDVFEGLHSFNEIEAIRFYSSEPAAFSKTLPVGKTVS
                     GEEDKP"
     gene            6666..7184
                     /gene="folK"
                     /locus_tag="PK125_05765"
     CDS             6666..7184
                     /gene="folK"
                     /locus_tag="PK125_05765"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HPB37648.1"
                     /translation="MRDRVFIGIGSNLGDSQANCITSIRELVKDRRLVFRDASSLYLT
                     SPVSSVPQDDFVNCAVFVSWTSSPLSLLELLMGIENRMGRVRDSRKDSPRVIDLDILL
                     YGNLILQEHSLIIPHAELHRRKFAMVPCLEIDPQIIHPGLKRPLATFLSDIDDQQSVE
                     RIRKITFDEIGL"
     gene            7184..7756
                     /locus_tag="PK125_05770"
     CDS             7184..7756
                     /locus_tag="PK125_05770"
                     /inference="COORDINATES: protein motif:HMM:NF006413.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HPB37649.1"
                     /translation="MIQKMFEQFKEIGRDIFLRGLITSHGGNMSARALNQIYITRRFS
                     MLGRLQEKDVIELELGQDNSQIPMASSELIVHTAVYRATGALAIIHTHPPYATLLSML
                     QNEIIPVDSEGLYLFGKTPVVSPKKTIGSEESAHLVSEQLKNHKIVLIRGHGSFARGE
                     TLEEAFMYTTSLESSSFFLWHLKNGYNRNR"
     gene            7799..8443
                     /gene="fsa"
                     /locus_tag="PK125_05775"
     CDS             7799..8443
                     /gene="fsa"
                     /locus_tag="PK125_05775"
                     /inference="COORDINATES: protein motif:HMM:TIGR00875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HPB37650.1"
                     /translation="MKFFIDTANVEEIKKGIEMGLVDGVTTNPSLLAKEKKDPQATIK
                     EILSVVEGPVSLEVIAKDSMGMCEEARKLAALGPNAVIKIPMTEEGIKAVRVLSREGI
                     KTNVTLMFQPIQALIAAKAGATYVSPFIGRLDDISTRGMGIIEDTITIFANYGYETEI
                     IVASIRHPLHVVEAAILGADIATIPFNVLRQLMNHPLTDIGLERFLKDWESLKK"
     gene            8576..11401
                     /locus_tag="PK125_05780"
     CDS             8576..11401
                     /locus_tag="PK125_05780"
                     /inference="COORDINATES: protein motif:HMM:NF020084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FIST N-terminal domain-containing protein"
                     /protein_id="HPB37651.1"
                     /translation="MGTYFEAGIGRSFRGQGIDMGRRAASQALSQLEKYRPSLAIVFA
                     CPELDLDAIQKGILETIGDCPLIGTSTAGEIANGLITRGVVVTVIASPHLKVSVGMGT
                     GVSKNFENAVNEALAQAKASKYFNPTHPLHQMLHISTSKRPRVSPVLAIMFSAGATKS
                     RFCLTHDIHTMLRKGSANRIPIFGGSSGDYFRFEATYQMANQHLSNDSVVLAFIESDI
                     LFGLGMAHGFSPTTKRALITKASGHIVYELDNRPAADVCSELLGVPRGQLGNDAIWFS
                     RYPFGTTDLYGNSILQVPEQVLDDGSIKFGPIMKTDQVITLMNGTKDDMRDAGLSAYN
                     KAILQGGLKKPALALMFSCALRMRLMGKDRRSEINSVLRKTKVPVAGFYTFGEQGITD
                     DCLPIYANQSVSTLVFSDELNPVATMIHKGKDIYFTFASQLNRKISQIKAITRINQIL
                     EDQTVVESLLAMLSKELPMVLQWADGAFYFPSKTAGTYILMGASDRRKFPEKIIPHET
                     GSHYTCIGLDIHSKHFGLMVLKQKDNELVPDDEDMLFAETVAKLVAGSLHKIDLDRGL
                     EAKMQHFEIFNQLGKELSHSIATTVQFQKLVEHIRCILKFSFVSLWLVDPTHQILVKE
                     AVDADPDVIFTGDKRENDERITKWQLTHCRLLSVADTEDKSNPVNLLHPFPFQFISLP
                     VFYKGALRGILNVYSFIKSNSFFEHVRLYDDENVGFLESLAAHISVLIGNRFIQKDTT
                     LFKEINHRVKNNLQNIASLLRMQIRRLDRVSPEQALSDSISRIMSIAVVHENLSHGEI
                     GMVNVGQLIASISQFSVTGNSEEPIITLDISGVPIMIPSKEATSLALVVNELIQNALR
                     HGILPQGRGKLSITISQTNGFISVIVHNDGPGISSDFDIEKRSNLGLTIVRTLVQNEL
                     KGSFTLVNSEGTVATVTFPFPETYVHA"
     gene            11414..11974
                     /locus_tag="PK125_05785"
     CDS             11414..11974
                     /locus_tag="PK125_05785"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HPB37652.1"
                     /translation="MFRILIADDEPLIRVDLKELLEEIGHQVVAETGDGKEALALLNT
                     VRPDLVILDIKMPGMSGIEVARRIAHEYPVIILTAFSERSLIEQAKDAGVMAYITKPF
                     RDGTLTPAIELAVSNYLEKAALSEKVSQLKEQLETRKVIDKAKGLLMDKQGLSEARAY
                     RHIQTVSMNKNRSMKEVAEAIILMLE"
     gene            12149..13381
                     /locus_tag="PK125_05790"
     CDS             12149..13381
                     /locus_tag="PK125_05790"
                     /inference="COORDINATES: protein motif:HMM:NF024825.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HPB37653.1"
                     /translation="MKVRTPFSLFTVFIALLALIVIFPISSYAAKDKIRIGTAISLSG
                     PYSAGAGMTQIPNYKLWVDEVNAKGGLYIKELKKKLPIELIIYDDKSDTGTVVKLVEQ
                     LILKDKVDFILPPWGTAMHFAIAPVINKYKYPVIGPTISSEKLREIAHKVPYFFGMLN
                     MPREQAAGLVEILEEVGVKKAALIYVADAYGIEWSSKVAPELGLKKIDMPIFKSYPLD
                     TKDISPLLKTIASADVDAVLCMSYPNDTFLITKQMKEMGYNPKLLYLGVGVAFPVYKQ
                     MFGGPDVIEGIMGAGAWNPKVPYPGAKDWYDRHVKKWNKEPDFWASAFAYASLQVLEQ
                     SIEKVGLDRKKVRDYIASNTFPTVIGPVRFEGGFNVQSPGEIGQWQKGQFEIVASKAK
                     RTAKPIYPKPPWPPEKAK"
     gene            13405..14283
                     /locus_tag="PK125_05795"
     CDS             13405..14283
                     /locus_tag="PK125_05795"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HPB37654.1"
                     /translation="MSFAFYCDVAFGGLVLGGLYALIAVGLSLQYGVARVLNVSHGEF
                     IMLGAFATYSLYTFLGINPLISLVITGPVIFLIAFLAHRTMFNRIRKTSPTIAAFEGN
                     SLLASFGLLFIIQNIALLGWGPDIKGFHYLSGSIKLFGNTYAINRIVALVLALIAGIA
                     FYLFLLNTRLGKAIRAAAQDRDTAQLMGVNIHKVLGFCFGLGAFMAALAGFLISMIFE
                     ITPTMGLPYTIIAIIAVVLGGLGNILGSLIGGLILGLVGNVVMCFNPALSMVAYYIIF
                     LLLILLRPSGILGRKT"
     gene            14280..15236
                     /locus_tag="PK125_05800"
     CDS             14280..15236
                     /locus_tag="PK125_05800"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HPB37655.1"
                     /translation="MNSLSTGKYLPNPRIPVLFSAAVLAAALLPLIMDPFTTISATNV
                     FIYIVITLSWALFSGPTQYISIATAAFFGIGIYTSALAGELMPFPLVVCLGGVVSFVL
                     ALLVGLSTLRLKGMYFTIFTFGLSELIRHFVLWWEVVMTGTVGRWVVSVDPIPVYYTM
                     LVILVVTISFCFFIGRSRYGLALRSIGQSEEAADHMGINITAVKINVFAITAFFAGAT
                     GSTMATRWTYIDPSIAFNPLFSFMPVLMAIFGGIGSITGQIIGAAVLTLLADLLLTEF
                     PYYYMLIYGIILVVVILFLPGGIVGLWDSMKKRRRTETNENT"
     gene            15223..15951
                     /locus_tag="PK125_05805"
     CDS             15223..15951
                     /locus_tag="PK125_05805"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPB37656.1"
                     /translation="MRILEVQGITVRFGGLVAVSGVDFHIDHGEVVGLIGPNGAGKTT
                     LFNLISGAITPSSGIILFKGDNITRLKPNEICRRGIARTFQLVKLFSDMTVLENVMLS
                     CLFRKPRVTKDEAAHESMELLDFVGLNGTESLPARELPIGGQKRLEVARALATKPELL
                     LLDEVMAGLNPAEVAEAIESIKKIREKGISIMLVEHVMQAIMDVSDRIIVLHNGEKLA
                     EGVPCDIASNETVINVYLGGTISC"
     gene            15945..16649
                     /locus_tag="PK125_05810"
     CDS             15945..16649
                     /locus_tag="PK125_05810"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPB37657.1"
                     /translation="MLKLTNISTSYGKVRVLREVSLEVAENEIVALVGANGAGKSTML
                     NTISGIVNPISGSIEFLGKRIDGMPPYDVLELGISQVPEGGKPFPDMTVRENLEMGAY
                     SKKARKFMGESLERVFKTFPILKERGKQLARTLSGGQRQMLAMGRCLMSRPRLAMFDE
                     PSYGLAPVVVGELFTFVQELRNQGIAILLVEQNIRHALETADRGYVLENGQIVLEGSS
                     RDLLSNDHVRKAYLGI"
     gene            16905..17687
                     /locus_tag="PK125_05815"
     CDS             16905..17687
                     /locus_tag="PK125_05815"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HPB37658.1"
                     /translation="MHSFKMFELDGKVAVVTGAASGLGAAFAEAMAEAGADVVCGDID
                     LKGMGKTVAEIKKKGHKAIAVKCDVSKEDDCVRLMQKTVKTFGHLDILINNAGIVESE
                     PKPLHECTTDAWNSVLAVDLHGVFFCTREALKFMVKQKSGKIINIASIFGITGSGHLF
                     PVPVYNAAKGAVVNLTRELGLEYAPLGINVNAICPGFFITNIGGGKSYTTEFDRMVSD
                     FTPMGRQALPEELKGTAIFLASAASDYMCGQMIVVDGGCTAK"
     gene            17698..18441
                     /locus_tag="PK125_05820"
     CDS             17698..18441
                     /locus_tag="PK125_05820"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPB37659.1"
                     /translation="MPHSDTLLRVDKISISFGGLAVLTDVNLKVDEGELVGLIGPNGA
                     GKSTLFNVISSIYKPDTGDVFLKGKKITGIAPHKLCRMGISRTYQLVKTFNKMTALEN
                     VMVGAIYGHKHGGSDAKTRAIDAMSLVGIEDKQNILVSHLTLSDRRLVEVARSLASSP
                     LVTLLDEPMAGLNVSEIKTLLTVIKRAREERKISILWVEHKVDAIFRTCDRVAVLNYG
                     AKIADGKPEEIAKDSKVIEAYLGESLTRN"
     gene            18416..19138
                     /locus_tag="PK125_05825"
     CDS             18416..19138
                     /locus_tag="PK125_05825"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPB37660.1"
                     /translation="MENRLLEIKDLHVSYGNVCALWGVSLHVPEGSIVALVGANGAGK
                     STLLKTIAGMNRPKNGSILFSGKHITGLTPEAVVNAGIAMVPEGRKLFARLTVKENLE
                     LGGYIPRARPFIQKSLEQAYELFPLLKTKSNQIAGSMSGGEQQMLAIARSMMSRPTIL
                     MLDEPSLGLSPLVVKMMFELVEELNRKGMTILLVEQNIYQALKISHYAYVIKTGKIVL
                     EGKGEQLLSDPEVQNAYLGTME"
     gene            19145..20038
                     /locus_tag="PK125_05830"
     CDS             19145..20038
                     /locus_tag="PK125_05830"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HPB37661.1"
                     /translation="MFDYLTFGLLAQLFINGILFGTMYGLAAIGLSLIFGTMRIIFLS
                     QGAVIILMAYCCYWLFTLFKIDPYLSIPIIGLAAMVLGAGIYQGLFRRAVKMEDKNIT
                     LLIAVGLMFLIENLMAVMWTPNPRSISTGYTSSSIPVGGLAISFTRLMGFVMAMLSTA
                     GVTLFLKRTLVGKAVRAASEDIEAARLVGISPHWVNTIAFAIGIGLCGIAGIALSTTY
                     PFDPYFGFIFSLKAMIALALGGLGSVFGALLGGIILGTLESWSSFFISGGWADAVSYG
                     VFLLVLLFKPEGLLVRSVKKV"
BASE COUNT         5633 a         4430 c         4621 g         5475 t
ORIGIN      
        1 tgctaaatag caattttcca taatcctgat gattaattac cacctgtgct ctggtgtttc
       61 aacaaaattt ttatggtatc tgctggtggc aggcagaacc atgatatgtt tacatatata
      121 tcatattttg caatctacga tgtttatttt ctcctcccct ttttttctac gggataacaa
      181 aatccatgac agcgaataag tgacaatttt taatccacct tctcattttc ttttgccgca
      241 ggtagctcca gatacagggc aggtatggtt ttacactgaa acatgtaccg tcacttggtt
      301 ccccagaggt atccatcctt aaacaaaacc tcattgagtg ataaaatagt gtaagctgtt
      361 gttttttaag cgcctttata tgcttgttca gatcaaacct gcagtctttc ttccatatac
      421 acccatagga tcacaggcat aaggatttct tcttgcaggc acctgcctga ggatattttt
      481 gtttatcctc cacttgctgg ccattgtatg ggggacaacc gatccttctg tatagggagg
      541 ttttaggcca aaacgactat ttcattttca aagttcttca aatctgataa aaacttggga
      601 aaggagcaag taatatggat aaaatcaaaa taggaatcag cagatgcctg cttggtgaga
      661 atgtacgata cgatggcgga cacaaacatg atcgctatct aactgacata ctgggcaaat
      721 atttcgagta tactccggtt tgcccagagg ttgagtatgg tcttccgatt ccgagggaac
      781 cccttcgcct tgtgggtaaa ccggaagatg cacgacttgt caccctcaat acagggattg
      841 atcatacgga tgggatgctg gaatgggcaa aggggaaatt gaaagagctt gaagaaaagg
      901 atctttgcgg atttgttttt aagagcaaat cccccagttc tggaatgcag tcggtaaagg
      961 tttatggacc atcaggcgtg cctgttcgca aaggtgtagg tatctttgcc ggcgctttca
     1021 cgaaacgttt ccctaatttg cccgtagagg aagatgaacg gctctataac acggtcctaa
     1081 gagaaaattt catggaacgg gtttttgtat ataaacgctg gcaggacatg ttacgtgaag
     1141 gaaaaaatat caaaaattta agagattttc acattgccca taagttcctc gttatgtccc
     1201 acagcccaag gcattaccgc aatcttgaaa aactaatggt agacgtcaaa agttataaaa
     1261 tcgacgttta cgaggaatat gtcaaaaccc ttatggaagg tctgcgcctt atagcgacgg
     1321 ttaagaaaca ggttaatgtt ctgcgccgca tcatgggata tttaaacaga tacctaacaa
     1381 cagatgaaaa acaggaggta caggaggtaa tacacaattt ccaccaacaa cttgtccctc
     1441 tcatcacccc cataaccctt ctcactcact acattcgaaa atacggcata tcccatcttg
     1501 aacgtcagca ttacctttac cctcaccatg tggaattaat gctgaggaat catatataaa
     1561 tgcagcgtcc agctcatagc tgaaagcaac aaaaaaacct ctttaacgtc aatcactcaa
     1621 agatttacag cattacttcc tttacgagct atcagctttg agctatgggc tgcttcgccc
     1681 atggcccatt gccgctgtta atcttgcacc tattcattaa atcaggtatt cttatccaat
     1741 aacatataaa agataggggg atttcatggg agtttacagc gacatctatg agtttgcagc
     1801 cagggctggt gcatttgaag gatatgtcta tcagagagag gggcttactg ctgagtcttt
     1861 ggaaagatgg gttgaccacc ttgttgaagg atataatgca gtggctccag atattaggaa
     1921 agaatttcag agcctatgtg atggtacaat aggtcgtgcc attcagtcgc ttatcataac
     1981 acttggagaa caccacgaaa taatcaggaa actaagaggg cttactacag gcaaattgcc
     2041 atcctcgcct gatgattttt cacggaaaag atgatctgcc agcctccaat tcaacaagtc
     2101 tttgtgcaac ctgtgctgcc agttgttcgt gcttaacata gagcatgagc tttttatcag
     2161 gatcttttcc tccggcatcc tccatcatag cttcctcttc tgagatgttc tcattcgatg
     2221 ggttcatccc tttgacgaat cctttcaccg tcatcactct tcctgtcaat ccgggccatt
     2281 gttggaccag ctttcctttc atgggcaatt cagcaacaag aataagatct gcccgggcat
     2341 gtttttcgtt aaagattgtt gaaaagtgag catcaatctc tgacataggg accccaagac
     2401 caagcaagaa agccttacga agcggattta cagtatcagc ctggctaaac cctgcagaca
     2461 ttacaatgat tttgcttccc agtcttttca gtttcagcct tacgctcatc tcgagatgaa
     2521 cacttctccc ctcattccat tcacacacaa aacagacaag tctgattggc ttcatctcta
     2581 ccatgttcga acccaacttt tttgtcacta ccagtacttt tagcttaatc tcattatcaa
     2641 tgcaagctta ctacgcgtct tatggttttt tctgtttctt aattctttct catactttac
     2701 ccactgaccc attctgcaca aacatggggc aacggtaatg atgaaaaaac ttgacttttt
     2761 cataatcacc gaataactta ataagtcaga cttgatataa ggaaaaagat atgagcggat
     2821 atctaaatcg aaaatgggag acattcccag tacacaagct taagagagta cctaacccaa
     2881 caacccttat tcatagtgac atggttcaaa gggttcggga gagagaggct ggattttgca
     2941 aatctgcagc aggagattat gggcctaaac ttgagacgga aacgcggcgt tttgtgccaa
     3001 aacatcctgc ctctggtgcg ctttcatgga tgagaaacaa catgaagccc tttttggacg
     3061 gtatcacctc agcccatcaa gccccaatac ctgatgaccc ggccagtctt gcccgtcata
     3121 tcaaagagat ggcatatttt ctgagggctg acactgttgg catatgtgag cttcctccat
     3181 atgctgtcta tagccataaa tttgatttta tgaagcctga agctgcagaa gtccccattg
     3241 aattgaatca caagtacgca attgccgtgc taatagatca ggattaccgc acatcccatg
     3301 ctacctcggg aagtgactgg atcagcaatg ccatgagtat gctggcttat acaacatcag
     3361 gcttcattgc tatcattctc gctgaataca tcagaaggct cggttatcct gcaaaagctc
     3421 actatgcgcc attttatgat cttgtggtgc cacctattct tctcctggca ggcctgggtg
     3481 agatgtgtag aataggagat atagtgctca acccctttct tggtccgcga ttcaaggcag
     3541 cagttgtcac aaccgatatg ccccttctgt cggataaacc cattgatttt ggccttcagg
     3601 acttttgctc aaaatgtaaa aaatgtgccc gtgaatgccc aagcggcgct attagtgacg
     3661 gtggcaaaga aatgtacaat ggttatgaaa agtggccaat ggataaaaag aaatgtacag
     3721 gcatgagggt aggaaaccag agaggatcag gctgcggtac ctgtataaag gtgtgcccat
     3781 ggaacaaacc ctatactccc tttcatcgat ctgtaggctg ggccatgaga aattcttcca
     3841 ttgcgagaag ttttgccatc tggggagatg atctcctcgg gtatggaaaa cctgattaca
     3901 gccaaaaatg gtggtttgac ctggaagatg tgaacggaga tggcgtgctg agtatccccg
     3961 caaacaaaaa gagcgagttc gaagggaggt aaattgaaac aacaacccat tcatcagaaa
     4021 aaggagggga agagcacggt aacgccaacc tttcaacctc tggacaaaag gagatacttc
     4081 gagcaggtgg ctcaactgat tcgaacgaaa atttttgacg aaggtttagt ggagggtttc
     4141 aggttgccgt ctgaggtcga cattgcaaac gaattgcgtg tcagtcgctc agtggtcagg
     4201 gaagcattgc gcatacttga cgtaatgggc tatatccatg ccaagaaagg cccccaggga
     4261 ggtatatttg taagctcaca ctatcacaaa ccggtcacag aatcattcag gtcgctggca
     4321 gaaaacggac agattactgt ggatcacctc tttgatgtgc gcttgcaaat tgaaccattt
     4381 attatcgaag aggctactgt tcatgcaaaa gatgaggata tcaaaaaaat tgctttgctc
     4441 tttcaagacg catcacgcgc tatggacgac gcagccaagc tcaaaagagc aaacatagac
     4501 ttccatttgc ttctcgcaga agcctcgggg aatcctgtcc tttccatcct catgaaatca
     4561 cttacagata ttctcatgga agtagcatac acttttcttg acctggattt cgaaagacaa
     4621 ctctttgaaa ttcacaagga aatcttccat gcaataaagt cgagaaaaac cggtgaagta
     4681 agcaggctca ttaacgctga tatcctgttc gtaaaagaga accttaaaaa ctatgtagaa
     4741 aggggggata tacgcaatgc cggagaacag gcactaaaag aaacacccgc atttaaaaga
     4801 aaaaagggag gttgatatga aacgtcatga actattgatt gggtttggtt tgttaatgtt
     4861 tttacttggt ttattcctct attgtcctgc tgtgcaggcc caggagaaag tcatcactat
     4921 gaactacgca catttcatgc caattatgac gaagcaggct cagctcgggg aacagtggtg
     4981 tcgtgaagta gaaaaaagaa cgaataacag agtaaagatc accttttatc ctgggggaac
     5041 ccttgccaca gcaccgctcg tttatgatgc cgtagtgaaa ggtattgccg atgttggatg
     5101 gtcgtttttg agttatacac gaggtcgctt ccctctcagc gaagtaattg accttcccct
     5161 ggggtacaag agtggttatg cagccacaaa gatgactaat gagtattata agaagtataa
     5221 gccgaaagaa ctcgatgatg ttaaggtcat gtacctccac gcccatggcc cgggcctcct
     5281 cagtaccaag aaagccgttt acaaactcag tgatttgaag gggatgaaga tacggtcgac
     5341 cggtctgagc gccaagatag ttcaagcact tggtggtgcc acggtgggaa tgtcaattgc
     5401 cgaggcatat gacgccctga ggacaggcgt tgtagatggg attcttattc ctgttgaagc
     5461 cttagaacaa tggaaacttg gtgagcttac ccagtttacg actgaaagct acagttcagc
     5521 ctatacaacg gcaggttttt gtgtaatgaa caaagcaaag tggaacagcc tccccgccga
     5581 catccagaac atcattgaaa aaataaatga ggaatggatt gaaaagaccg gattgttgtg
     5641 ggatcagatg gacaaggatg ggaaagcatt tgcattgagt aagggaataa agtttatacc
     5701 cctctcacaa gaggagagcg ctttatgggc agcaaaagtg aagccaattg ttgatgaata
     5761 cgttcagaca atgaaaagca agaacctgcc tggcgaagaa gtattaaagt tttgtcagga
     5821 ttatctaaca aaaacccaaa aataaaaccg acttgggggc atccctttcc tgtcactgtc
     5881 agtgtgaaag gatgccccca cagctcgctg agccgatata acttgttcaa atgcgccgac
     5941 ctgttatttt cagtaatacc ccgtgtggag gaatggtaga attcattcgt tttagccttt
     6001 ctcgcctgtc ccgccttatc ttctcacttt tcacttttca atttgtattt tttacttcct
     6061 ttccctccgt tcttgttcgt cctgcttttc ccgtcaattt cggtgcatct gatcttcaat
     6121 tctgctataa tggcacttat aataaggcac tgtcgcgagg attctccatg atctctaaag
     6181 ggtggcacag gttggtcagt atcatatcag ttgtaggggt tataggcctg cttttagctt
     6241 tccctctcct atccagggca gtagaagtag attctgaact gatggaacac tttaaaacaa
     6301 atgagacagg gagctacgtg atatattttc gagcaaaagc aagcttcgcc ggtgcaccaa
     6361 actcgagctg gaaggaacag aacgagttta tcaaccgggc tcttcaggac aatgcaaatc
     6421 gttcacaggc tcgtgtcgta tcgtatttgt ttcatcacag ggttcagtac cgcagtctct
     6481 ggagggacaa cacagtaatt gtggagaaat caaacaagga tgtctttgaa ggtctccact
     6541 ctttcaatga aattgaggct atccggtttt acagctcaga accggcggcc ttttccaaaa
     6601 ccttgcctgt cgggaaaacc gtgtcaggtg aagaagataa accttgagaa aggaagcatg
     6661 agaaaatgag agatcgggta ttcataggta tcggatccaa tttgggtgac agccaggcaa
     6721 actgtattac aagcatcagg gaattggtaa aagataggcg tcttgtcttt cgggatgcct
     6781 cctccttgta ccttacatcc cctgtttctt ccgttccaca agacgatttt gtcaattgtg
     6841 cagtctttgt atcatggacg agctctcctc tctcacttct cgaactttta atgggcatag
     6901 aaaaccgtat gggaagggtg cgggacagcc gaaaagacag tccacgggtg attgatcttg
     6961 atattctcct ttatggcaac ctcattcttc aggagcactc gttaatcatt ccccatgcag
     7021 aactccacag aagaaagttt gctatggttc cttgtcttga aattgatccc cagataatac
     7081 accctggttt aaaacgcccc ctcgcaacct ttctcagtga tatagacgat cagcaatctg
     7141 ttgaacgtat cagaaaaatt accttcgatg agatagggct ttgatgatac agaaaatgtt
     7201 tgagcagttc aaagagattg ggcgagatat ttttttgaga ggtctcatca cctctcacgg
     7261 cggcaacatg agtgcccgtg ccctgaatca gatctacatt acacgaagat tctccatgct
     7321 gggcaggtta caggaaaaag atgtgatcga acttgaactc gggcaggata actcacaaat
     7381 ccctatggct tcgtcagagc taattgtcca cacagccgtt tacagggcaa ctggtgctct
     7441 cgccattata cacacccatc ccccctacgc aacgctttta tccatgcttc agaatgaaat
     7501 tattccagtg gactcggaag gattatattt gttcggaaaa acacccgtag tgagtccgaa
     7561 aaagactatt ggctctgaag aatctgctca tcttgtaagc gaacaactca aaaatcacaa
     7621 aattgttctc atacggggtc acggaagttt tgcaaggggc gaaacgcttg aagaggcatt
     7681 tatgtatacg accagccttg aatcatcaag cttttttctg tggcatctca aaaatggtta
     7741 caatagaaat agatgaaaaa catacattct ttaaaaaaat gaatgggtag gagggcagat
     7801 gaaatttttt atcgatacgg ccaatgttga agaaattaag aaagggattg aaatgggact
     7861 tgtcgatggg gtaacaacaa acccatcgct tcttgcaaaa gaaaaaaagg atccccaggc
     7921 aaccatcaag gaaatacttt ctgtggtaga aggaccggta agccttgagg taatagccaa
     7981 agattctatg ggtatgtgcg aagaagcccg aaaacttgcc gctctgggtc caaatgccgt
     8041 tataaagatt cctatgaccg aagaaggcat taaagcagtt cgtgtattat cccgggaagg
     8101 cattaaaaca aatgtaaccc ttatgttcca gcctatccag gctctgatag cagccaaagc
     8161 aggcgccact tacgtcagcc cgttcattgg ccgtcttgat gatatctcaa cacgggggat
     8221 gggcatcatc gaggatacaa tcaccatctt tgcaaattac ggttacgaaa cagagatcat
     8281 tgtagcgagc atcagacacc cgctccatgt tgttgaggcg gcaatccttg gcgctgatat
     8341 cgctaccata cccttcaatg ttctcagaca gttgatgaac catccgctga ccgacatcgg
     8401 tcttgaacga tttctgaagg actgggagtc actgaaaaag taagatatac agcagcgctg
     8461 ctctcatcca tcgataccgt ctttaaatgt tcggtaaata gcgtattgtc gttctccttt
     8521 tgtatttccc tatacgatat gctatatacg agagacgacg ttaacaggac tgcaaatggg
     8581 aacatatttt gaagcaggga tcggcaggtc tttccgtggt cagggcatag atatgggcag
     8641 aagggcagcc tctcaagccc tttctcaact cgaaaaatat aggccatccc ttgcaatcgt
     8701 ttttgcatgt ccggaacttg accttgatgc catccagaaa ggtattttag agaccattgg
     8761 cgattgtccg ctcatcggta caagtacggc cggcgaaatt gcaaatggtc ttattaccag
     8821 aggcgttgta gtaacagtca tagcgtcccc tcacctcaaa gtctcagtgg gtatgggaac
     8881 aggtgtcagt aaaaatttcg aaaacgctgt taatgaggca cttgctcagg ccaaagcatc
     8941 gaaatacttc aatccaaccc acccgcttca tcaaatgctt cacatctcca catcaaaaag
     9001 accgagggtc tctcctgttc tggcaataat gttttcagca ggggctacaa aatcgcgttt
     9061 ctgtttaacc catgacatcc acaccatgct tcgcaagggt tctgccaacc gtattcctat
     9121 tttcggtgga agttccggag attactttcg ttttgaggca acttatcaaa tggccaatca
     9181 gcacctttcc aatgattcgg tcgtgcttgc tttcattgaa tcggatattc tgtttggact
     9241 gggtatggca catggctttt caccaacgac aaaacgtgct ctcattacca aggcatctgg
     9301 ccacatcgta tacgagctgg acaaccgtcc ggcagcagac gtgtgttccg agttgttggg
     9361 tgtccctcgc gggcagttag gtaacgatgc catatggttc agccgatacc cttttggcac
     9421 tacagacctt tacggcaact ccatcttgca ggtgcctgaa caggttctgg atgacggttc
     9481 gataaagttc ggacctatca tgaagactga ccaggtcatt acgcttatga atggaacaaa
     9541 agatgacatg cgggacgccg ggctttctgc atacaacaaa gccatactcc agggaggttt
     9601 gaaaaaacct gcccttgccc tcatgttttc gtgtgccttg agaatgcgac tgatgggcaa
     9661 agatagaagg tctgaaatca actcggtatt gaggaagaca aaggtaccgg ttgccggttt
     9721 ctacacattt ggcgaacaag ggataaccga cgactgtctt cccatctatg caaaccagtc
     9781 tgtttccacg ctagtcttct ctgatgagct aaatccagtt gcaaccatga ttcacaaagg
     9841 gaaagacatc tatttcacat tcgcatcgca actaaatcga aagatttccc agataaaggc
     9901 aattacaaga attaatcaaa ttttggagga tcaaactgtc gtcgaatctt tacttgcaat
     9961 gctctcaaag gaacttccca tggttctcca gtgggctgat ggagcttttt attttccttc
    10021 caagacagca ggcacttaca tccttatggg tgcatcagac agacgcaagt ttccagagaa
    10081 aattatcccc catgaaacag gcagtcacta tacatgcatc ggccttgaca tccatagcaa
    10141 gcactttgga cttatggtgc tcaaacaaaa agataatgaa ctcgtgcctg acgacgaaga
    10201 tatgcttttt gccgaaacag tcgccaaact ggtcgcagga agcctccata agatagactt
    10261 agacaggggt cttgaagcaa agatgcaaca cttcgaaata tttaaccagt taggcaaaga
    10321 gctatcccat tccatcgcca caactgtcca gtttcaaaaa ctcgttgaac acatccggtg
    10381 cattctaaag ttttcattcg taagcctctg gctggttgat ccgacccatc aaatactcgt
    10441 aaaagaagcg gtagatgctg acccggatgt catattcaca ggtgataaaa gagagaatga
    10501 tgaacggatt acaaaatggc aactcaccca ttgccggctg ctgtcagtag cagacacaga
    10561 agacaaatcc aaccctgtaa atcttctgca cccgttccct tttcagttta tctctttgcc
    10621 tgtcttttac aaaggcgctt tgaggggcat cttgaacgtc tattctttta taaaatcaaa
    10681 tagttttttt gaacatgttc gtctttatga tgatgaaaat gtcgggtttc tcgagagcct
    10741 tgcagcccac atttctgttt tgattggcaa tcggtttatc cagaaagaca cgacattatt
    10801 taaagagatc aaccaccgtg taaagaataa tcttcagaac attgcaagtc tcctgcgaat
    10861 gcagatcaga cgccttgaca gggtttcacc cgaacaagca ttgtccgaca gtatttcgag
    10921 gatcatgagc atcgccgttg ttcatgaaaa cctctcccat ggagagattg gcatggtgaa
    10981 cgtgggacag cttatcgcaa gcattagtca gttttcggta acaggtaatt cagaagaacc
    11041 cataataacc ctcgatattt caggtgtacc catcatgatc ccctcgaaag aggcgacctc
    11101 tcttgccctc gtggtcaatg agctcatcca gaacgctcta cgtcatggca ttctcccaca
    11161 aggcaggggc aaactatcta tcacaatctc ccaaacaaat ggctttatct ctgttatcgt
    11221 ccataatgat ggtcctggaa tttcatcaga ttttgatatt gaaaagagaa gcaaccttgg
    11281 tctaacaatt gtgcggaccc ttgttcaaaa tgaactcaag gggagtttta ccctggttaa
    11341 ctcagaagga acagttgcaa ctgtaacgtt tcccttccca gaaacatatg tacatgctta
    11401 agaggagtat tacatgttta ggatattaat tgctgatgat gaacccctca tcagggtcga
    11461 tttgaaagaa cttttggaag aaataggtca tcaggtggtg gctgaaaccg gggatggcaa
    11521 agaggccctc gcccttctca atacggtacg ccctgatctg gtcatacttg acattaagat
    11581 gccgggcatg agcggtatcg aggtggcccg aagaatagcg cacgaatacc cggtaatcat
    11641 cctcacagct ttttcagagc gttcattaat cgaacaggca aaagatgccg gagttatggc
    11701 ctatatcaca aagccgtttc gtgatggtac gctaacacca gccattgagc ttgcagtgag
    11761 caattatttg gagaaagctg ctctttcgga gaaggtaagc cagctcaaag aacaactcga
    11821 aacacgcaag gtcattgaca aggcaaaggg gctactgatg gacaaacaag gccttagtga
    11881 agcccgggca taccgccaca tacagacagt gagcatgaac aaaaacaggt caatgaaaga
    11941 ggttgctgaa gcaatcattt tgatgctcga ataaaaaaac atgttgacag aactttgcca
    12001 taaagttata agaagcataa tacgtatttt ttagaggtaa cgaagcctcc tttgtccgaa
    12061 gcagggcaaa gaaggctttt tatttggggc cgttgatcat tagacgttgg aattatgaag
    12121 taaaaaattt aatctaaagg gggttaaaat gaaagtaaga acaccatttt ctctgtttac
    12181 ggtcttcatc gcactgctcg ccttgatagt gatctttccc ataagtagct atgctgcaaa
    12241 agacaagata cgcattggca cagcaatctc cttgtctgga ccgtactcgg ccggcgctgg
    12301 aatgacccag attcccaatt acaagctctg ggtagacgag gtgaatgcta agggtggtct
    12361 ttatatcaag gaactgaaaa agaagttgcc cattgaactg atcatttatg atgacaagag
    12421 tgatactggc acagtcgtga aactggttga acagctaatc ctgaaggaca aggttgattt
    12481 tattctgccc ccctggggta cagcaatgca ctttgcaatt gctcctgtaa tcaacaaata
    12541 caagtatccg gttatagggc caacgatatc ctctgaaaaa ctccgagaaa tagcccacaa
    12601 ggtaccgtat tttttcggta tgctcaacat gccacgagag caggcagcag gcctggttga
    12661 gatacttgaa gaagtgggcg tgaaaaaagc tgccctcatt tacgtagcag atgcatatgg
    12721 catagaatgg agcagtaaag tagcacctga actgggcctc aaaaaaatag atatgcctat
    12781 tttcaagagc tatcctctgg acactaaaga tatctcgccc ctgttaaaaa caattgcgag
    12841 tgctgatgta gatgcagtgc tttgtatgtc atatcccaat gacactttcc ttattacaaa
    12901 acagatgaaa gaaatgggtt acaaccccaa gcttctctat ctcggcgttg gcgtagcctt
    12961 tcctgtatat aagcagatgt ttggaggccc cgatgtcata gagggcatca tgggagccgg
    13021 tgcatggaac cccaaagtcc cttaccctgg cgctaaagac tggtatgaca gacatgttaa
    13081 aaaatggaac aaggagcctg atttttgggc tagcgccttt gcatacgcat ctctgcaagt
    13141 cctcgaacag tccatagaaa aggtcggttt agatcgcaaa aaggttcggg attacattgc
    13201 ctcaaacacc ttcccgactg taatcggacc tgtaagattc gaaggtgggt ttaatgtaca
    13261 gtctcctgga gaaatcggac agtggcagaa gggccaattt gaaattgtag caagcaaagc
    13321 aaaaaggacg gcaaaaccca tatatccaaa accaccatgg ccaccggaaa aagcaaaata
    13381 gggcgtttgt gagaatatac cggcatgtca tttgcctttt attgtgatgt agcatttgga
    13441 ggcttagtcc tgggtgggct ttatgcgctc atagctgtag gattaagcct ccaatatggt
    13501 gttgcaaggg tactgaatgt ttctcatggt gaattcatca tgcttggcgc ctttgcaact
    13561 tattccctgt ataccttcct aggtatcaat cccctgatct cccttgttat tacagggcct
    13621 gtaatttttt taattgcctt tcttgcccat agaacgatgt ttaaccgtat cagaaagaca
    13681 tcgccgacaa ttgcagcttt tgagggcaat tcactccttg cgtcatttgg ccttctcttt
    13741 attatccaaa atattgccct ccttggctgg ggtcctgata ttaaaggatt ccactatcta
    13801 tcggggtcta ttaagctgtt tggaaatacg tatgccatta accgaatcgt agcactcgtt
    13861 ttggcgctga ttgcgggtat tgcattttat ctttttctcc tcaacacccg cctcggcaag
    13921 gcaattcgcg cagcagcaca agacagagat acggcacaac tcatgggggt aaacatccat
    13981 aaggttcttg gattctgttt tggtcttggc gcctttatgg ctgctctggc tggattcctt
    14041 ataagtatga tctttgagat tacacctacc atggggctac catatacaat aattgctatc
    14101 attgctgttg tgttgggagg gcttggcaat atcctgggca gcttgatagg aggacttatc
    14161 cttgggcttg taggtaatgt ggtcatgtgt ttcaaccccg ctttgtcaat ggttgcctat
    14221 tacataatct ttctcctgct aatactgcta agaccttcag gaatattggg aagaaagacg
    14281 tgaatagtct atctacaggt aaatacctgc caaatccccg tatccctgtg ttgttctcgg
    14341 cggctgttct tgccgcagcc cttctccctc tgatcatgga tccttttacc actatatctg
    14401 ccactaatgt cttcatctat attgttatta ccttgagctg ggctcttttc tccggaccta
    14461 cccagtatat atccattgca acggcagctt ttttcggtat tggaatatac acttcagccc
    14521 ttgcaggaga gttgatgcct ttcccgctcg tggtctgtct gggcggcgtc gttagctttg
    14581 tccttgcgct tcttgttggc ctttcaacgc ttcgactaaa gggtatgtac ttcaccatat
    14641 tcaccttcgg gttgagtgag cttatccggc attttgttct ctggtgggaa gtggtaatga
    14701 ctggtactgt aggcagatgg gttgtgtctg ttgaccctat accagtctac tatacgatgc
    14761 ttgtcattct tgttgtaacc atatcattct gtttttttat cggtcgttcc cggtatggtc
    14821 tggccctgcg gagcattggt cagtcagaag aggctgcaga ccacatgggt attaacataa
    14881 cagccgtcaa gatcaatgtt tttgctatca cagccttttt tgcgggagca acaggctcca
    14941 ccatggctac acgatggacc tacattgacc cttcaattgc ctttaaccct ctcttctcgt
    15001 tcatgcctgt actcatggcc atattcgggg gtatcgggag cattactggc caaataattg
    15061 gcgctgcagt tctaactctc ctggcagatc ttctcttaac ggagtttccc tattactaca
    15121 tgcttattta cgggataatc ctggtggttg tcatcctttt ccttcccggt ggcatcgttg
    15181 gactttggga ttctatgaaa aaacggagac gaacagaaac aaatgagaat acttgaagtt
    15241 caaggcatca cagtacgctt cggcggtttg gttgcagtat caggtgtaga tttccacatc
    15301 gaccatggtg aggtggtagg tttaataggc ccgaatgggg caggcaaaac cactctcttc
    15361 aacttaatat ccggtgcgat tacgccttct tcaggcataa tcctttttaa gggtgataat
    15421 attactcgtc ttaaaccgaa cgagatttgc aggcgaggta tagcgaggac ttttcagctt
    15481 gttaaactct tcagcgacat gactgttctg gaaaatgtaa tgttatcctg cctttttcga
    15541 aaaccgcgcg taactaaaga tgaagcagca cacgagtcaa tggaactgct tgacttcgtc
    15601 ggtctcaatg gaacagaatc tcttcctgcg cgagaactgc ctataggcgg tcaaaaacgc
    15661 cttgaggtgg caagagcatt ggcaaccaaa cctgaattgc ttttgctcga tgaagtaatg
    15721 gcaggattaa atccagctga agtggcagaa gccattgaat ctatcaaaaa aatacgagaa
    15781 aaaggcatca gcataatgtt ggtcgaacat gttatgcagg ccattatgga tgtttctgat
    15841 aggatcatag tacttcataa cggagaaaaa ttggctgaag gtgtaccgtg tgatatcgca
    15901 tcaaatgaaa ccgtaatcaa cgtatacctg ggtgggacaa tttcatgttg aaactgacta
    15961 atataagcac atcctatggc aaggtcagag tcctgagaga agtctccctg gaggtggctg
    16021 aaaatgagat tgtagccctt gtcggtgcca acggcgctgg aaaatcgacc atgctcaata
    16081 ctatttcagg gattgtgaat cccatatccg gttcaattga gtttcttggc aaacgtattg
    16141 atgggatgcc tccttacgat gtacttgagt tgggtatctc ccaggtgccg gaaggtggta
    16201 aacctttccc agatatgaca gtgcgggaaa acctggaaat gggtgcctac agtaaaaaag
    16261 cccggaaatt tatgggtgag agcctcgaac gtgtgtttaa aactttcccc atactgaagg
    16321 aacgggggaa acagttggca agaacgctga gcggtgggca gcgacaaatg cttgccatgg
    16381 gacggtgtct catgtcaagg ccacgtttag ccatgttcga tgagccatcc tatggacttg
    16441 ctccagtagt ggtaggtgag cttttcactt ttgttcaaga actgcggaac cagggcatag
    16501 ccattctact cgttgagcag aatattcgtc acgcccttga gactgcagat cgtggctatg
    16561 ttcttgaaaa tggtcagatt gtcctggagg gctcaagcag agatttactc tcgaatgacc
    16621 atgtaagaaa agcatacctt ggtatatgaa ataaattacg aaatgcttga catggattcc
    16681 gtaatgcaca tataatttgt tagtaatata taaacaaaaa attattgcga tatgcaggag
    16741 gtaaagaagc ctcctttgtc tgttgaagga caaaggaggc tttttgtttg tatgactgac
    16801 aatttagcta gatatcgggg agtaagatca ctaaaaaagg agcagtcaag acagagtatg
    16861 aacatcgctt tatattatgg gttcgactgt gagtgaggtg cataatgcat tcctttaaaa
    16921 tgtttgaact ggacggaaag gttgccgttg tgacaggtgc agcctcaggg ttaggcgcag
    16981 catttgcgga ggcaatggct gaggctggag cagatgtggt ctgtggggat attgatttaa
    17041 aaggcatggg taagaccgtt gcggagataa agaaaaaagg acacaaagcc atagctgtca
    17101 aatgtgacgt gtcaaaggag gatgattgtg tccggctcat gcaaaaaacg gtgaaaacct
    17161 tcggccattt ggacatcctc atcaacaacg caggtatagt cgaaagcgaa ccaaaacctt
    17221 tacacgaatg cacaacagac gcgtggaaca gtgttttggc tgttgacctt cacggtgtct
    17281 ttttttgcac gagagaggct cttaagttca tggttaagca gaagagcgga aaaatcatca
    17341 acattgcatc tatctttgga atcactggtt caggacatct attcccggtt cctgtatata
    17401 acgccgccaa aggggctgtt gttaacctca cccgcgagtt aggccttgaa tatgcccctc
    17461 tgggtataaa tgtgaatgct atatgtccag gcttcttcat cacgaacatc gggggtggca
    17521 agagttatac cactgagttt gacaggatgg tttctgactt cacacctatg gggaggcagg
    17581 ctttacccga ggaactgaaa ggtacagcca ttttcctggc atcagcagca tctgactata
    17641 tgtgcgggca gatgattgtg gtagatggag gatgtacggc aaaataggag atttttcatg
    17701 cctcacagcg atactcttct aagagtggac aaaatcagta tatcatttgg tggtctggca
    17761 gtcctgaccg acgtgaatct gaaggtggat gagggagaat tggttggcct gatcggacca
    17821 aacggtgccg gtaagtcgac ccttttcaac gtaatcagct ctatatacaa accagatacg
    17881 ggcgacgttt tcttaaaagg aaaaaagatt accggcattg cacctcacaa actttgccgc
    17941 atgggtatct ccagaacgta tcagcttgta aaaaccttca ataagatgac agctcttgaa
    18001 aatgtcatgg tcggggccat ctacggacat aagcacgggg gcagtgacgc aaagacaagg
    18061 gcaattgatg ctatgtcact cgttggcata gaagataagc agaacattct cgtatctcac
    18121 ctgacattgt cggatcgcag actggtggaa gttgccagat ctcttgcatc ctctccgctg
    18181 gtaactttgt tggacgaacc aatggcagga ttgaacgtat ctgaaatcaa aacgcttctt
    18241 acagtaataa agcgtgcccg tgaagagaga aagatatcca ttctgtgggt ggagcacaag
    18301 gttgatgcta tctttcgcac atgtgacagg gttgctgtcc tgaactacgg agcaaaaata
    18361 gcagatggaa agcctgaaga aatagcaaaa gattcaaaag tgatcgaggc atatcttgga
    18421 gaatcgctta ctcgaaatta aggatttgca tgtatcttat gggaatgttt gtgccctgtg
    18481 gggtgtgtcc ctgcacgtgc cagagggatc cattgtagcc ctggtgggtg caaacggagc
    18541 aggaaaatct acccttctga aaaccattgc cggaatgaac cgtcccaaga atggcagcat
    18601 cttgttctca gggaaacaca taacaggtct tacgcccgag gctgtggtaa atgccggtat
    18661 tgccatggtt ccggaaggcc gtaaattgtt tgcccgtttg accgtcaaag agaatctcga
    18721 actgggaggc tacatcccac gggcaaggcc tttcatacag aaatctttgg aacaggcata
    18781 cgagttgttc cctctgctaa agacaaaatc caaccagata gcgggctcca tgagtggcgg
    18841 agagcaacaa atgcttgcaa tcgcacgatc aatgatgagc agaccgacaa ttcttatgct
    18901 tgatgaacct tcacttggtc tcagccctct cgttgtcaag atgatgtttg agctggtgga
    18961 agaacttaat cgcaaaggaa tgactattct tcttgttgaa cagaacatct accaggcact
    19021 gaaaatctct cattatgcct atgtgatcaa gaccggtaag attgttcttg aaggcaaggg
    19081 agaacaactc ctttcagatc cggaagtaca aaacgcctat ttaggaacca tggagtagat
    19141 acttatgttc gattatctca cattcggtct tctcgcacag ctctttatca acggcattct
    19201 tttcgggaca atgtatggcc ttgctgccat cggcctcagt cttatcttcg gcacgatgcg
    19261 cataatcttt ctgtcccagg gtgcagtcat aatccttatg gcgtattgtt gctactggct
    19321 ctttacgctt ttcaagatcg atccttacct gtccatcccc ataattggat tggctgccat
    19381 ggtcctgggt gcaggtatat accaggggct ctttagacgg gcagtaaaaa tggaagataa
    19441 aaatataacc cttcttatcg ccgtcgggct catgtttctt attgagaatc tgatggcagt
    19501 gatgtggacc ccaaatccaa ggtccatatc aacgggatat acttcatcat caatccctgt
    19561 aggcggcctc gcgatttcat ttacccgcct catgggattc gtaatggcta tgctttcaac
    19621 cgctggtgta accctttttc taaaaaggac actcgtgggc aaggctgttc gggccgcttc
    19681 ggaagacata gaggcggcaa ggctcgtcgg cataagtcct cattgggtca acacaatagc
    19741 ttttgctatc ggaatcggtc tgtgcggtat agcaggcatt gctctgtcga ctacttaccc
    19801 tttcgatcct tattttggtt ttattttcag tctgaaggct atgatcgctc ttgccctcgg
    19861 aggcttaggg agcgtgtttg gggcactgct tggaggcatt atcctgggaa cacttgaaag
    19921 ttggtcttca ttctttatca gcggtggctg ggccgacgcg gtcagctatg gagtatttct
    19981 tttggtgtta ttgtttaaac cggaaggtct cttggtccgg tcagtaaaaa aggtttagaa
    20041 gatgaaaaac ttgaaaccta taaccagtgt tgttctggcc actctgttgc tgggattaat
    20101 cccctttgtc tctgattctg aaaaatcgta cctggtctat ttcctttttc tcacctttt
//
LOCUS       DAOOMC010000042        19822 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdus sp. isolate AMR_MDS_2685
            NODE_2734_length_19822_cov_6.464916, whole genome shotgun sequence.
ACCESSION   DAOOMC010000042 DAOOMC010000000
VERSION     DAOOMC010000042.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864760
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdus sp. (wastewater metagenome)
  ORGANISM  Syntrophorhabdus sp.
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae; Syntrophorhabdus.
REFERENCE   1  (bases 1 to 19822)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19822)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,729
            Genes (coding)                    :: 2,708
            CDSs (with protein)               :: 2,708
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 7 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19822
                     /organism="Syntrophorhabdus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2734_length_19822_cov_6.464916"
                     /isolate="AMR_MDS_2685"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2699756"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(326..1225)
                     /locus_tag="PK125_05835"
     CDS             complement(326..1225)
                     /locus_tag="PK125_05835"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HPB37662.1"
                     /translation="MSKKNQYDIVFVGQMGMGTVVPFEGVPFVILGSPVLFASIAASC
                     VGKRIAVVTTISKKEEYLLEPMKKAGIDLYIQPGETAQYRVVFPNANVDERQAFHVKG
                     GSNIEKVPPFEPCLLHCCCMGPREAQMDLMRSLKVRGFRLSVDMQGLMLQADHETGIV
                     RLEDVPEKKEILRMADFVKLDSKEAQALTGTDVLQDQAAILAGWGSPETIITSSGGVL
                     ARSHGTTKYAKFSNRSTKGRMGRGDTVIGSYIARRFDYPVEDSLQFAGALASIKMESV
                     GPFAGSLEDVIERMGDSVPCQSI"
     gene            1503..2162
                     /locus_tag="PK125_05840"
     CDS             1503..2162
                     /locus_tag="PK125_05840"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012387.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HPB37663.1"
                     /translation="MEYKISKGFVVKAGVRLMMLILATNLFLHGVLLFAAEKSSQKGE
                     KKMSDTQLKATIHTTKGDIRLNLFPGKAPLTVLNFVNLAKRGFYNGLSFHRVIPDFMI
                     QGGCPLGTGTGGPGYRFTDEFSSDLKHSKAGILSMANAGPNTNGSQFFITHVPTPWLD
                     NKHAIFGEVVDEKDQTVVNSIVKGDKITSISIDGDYSILADQNKDTLNKWNEVLEKQA
                     R"
     gene            complement(2274..3530)
                     /locus_tag="PK125_05845"
     CDS             complement(2274..3530)
                     /locus_tag="PK125_05845"
                     /inference="COORDINATES: protein motif:HMM:NF018485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPB37664.1"
                     /translation="MKSNALDKNQAIHWAWVILVICFINLFVNYGIRLGYSVVLPEMI
                     RTLGFTRRQAGDIFNAYFLAYIILSLFAGNLTDRLGARKIIPMFGVVLGIGTLLMGTS
                     TSFWQASLFFGIVGMGAAAMWTPIITLVQRWFAVKRRGMALGILSTGFGLGFASMGRL
                     FPIIVAEWNWRYCWYFLGIAALIMVLVNIFLLRSKPEDRGLVPWGTPAGEIIPAPQAT
                     TSQAVKTRYSEILTKGRFWMLGFSYLLVAGSLYIPTTFMVDYARYELGFPYERASLLA
                     TIHGIGQIVGVLTIGMISDYIGRRMTILFSNFCVGVAIVCIIASGGNVVWLFTSVGAL
                     GAFFGATFPMYGAAGGDYFRKEIMGTVIGALTIFYGTGAILAHRFAGHIRDATGSFSI
                     PFIIAVVASVIAAVLMGFVKRPEEAR"
     gene            complement(3554..3679)
                     /locus_tag="PK125_05850"
     CDS             complement(3554..3679)
                     /locus_tag="PK125_05850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB37665.1"
                     /translation="MKTGERIEATGKGSRFMVKGAIDKAEGFPAFILVSLTFNPG"
     gene            complement(3676..5580)
                     /gene="cooS"
                     /locus_tag="PK125_05855"
     CDS             complement(3676..5580)
                     /gene="cooS"
                     /locus_tag="PK125_05855"
                     /EC_number="1.2.7.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018492 - carbon-monoxide dehydrogenase
                     (acceptor) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic carbon-monoxide dehydrogenase
                     catalytic subunit"
                     /protein_id="HPB37666.1"
                     /translation="MEDERISYHESIRTMYQRIKQDGMTNMWDRYEAQGMGGNPDQRC
                     SFCQGGVRCDLCSNGPCRADVAKDKRGVCGITGDGMAMRMMLLRNVMGASTYHYHTEQ
                     TIKTLKAAVNGKAPFAITEPGKLIAFAERFDIPTSLSDEEVALALCNYVEADFNRKYD
                     EPSYIVETLAPKERKAVWKKLGIFPGGIYGEMMFATSSCLTNVDGYYASLALKAMRLG
                     IAMAYQSQIVNEFCQDILFGIPRPHPMRVDLGVLDPEYVNVLPNGHEPFLGFTMVELA
                     RKNEWQEKAKAVGAKGVRVIANIETGQEMIQRWQMDDVFYGFTGNWIMQEAILASGCI
                     DLFACDMNCSMPVDPFYAEKYKFKLIPVSQLVAFEGITDRIDYVPEKASEQAASLLQM
                     AIDNFKERHATIKPITGLNMGEAIVGFSTESIAAALGGSVLPLLKAIQDGVIRGVAGL
                     VSCTSLRDTGQDVHSVTIAKELIKRDILVLSMGCGNAAMQVAGLCSPEAKDFAGNGLR
                     ALCETLKIPPVLSYGTCTDTGRLADMLAAIANVMQVPIPDLPVAAVAPEYMEQKATID
                     AVFALALGLYTYVNPVPTVTGAPNLVQLLTVDLPQVTGGVLSVEKDPIKAVDGVLAHI
                     EAKRKKLGMQ"
     gene            complement(5702..6166)
                     /locus_tag="PK125_05860"
     CDS             complement(5702..6166)
                     /locus_tag="PK125_05860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB37667.1"
                     /translation="MELYEILPECRNFIKPMADVRLITRGYHTIEDSFHPLLWFSPKG
                     YTAHYYLHPEARSAERFQQLLNNHEEVKLLEVRFEDAMLDSWERQYSLSVENCTLWDC
                     VSLRHDYDVIFAETKNILKYDNGTLLKAYDRIIASVEEACMKFYHLCRFTQS"
     gene            complement(6234..6611)
                     /locus_tag="PK125_05865"
     CDS             complement(6234..6611)
                     /locus_tag="PK125_05865"
                     /inference="COORDINATES: protein motif:HMM:NF020473.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1844 domain-containing protein"
                     /protein_id="HPB37668.1"
                     /translation="MDDDQKGFKITDKRASASEAEAVDPEKKQDERTCAEIAEEEAFT
                     TVLNFSTFILSLTTSALVCLGELPDPITKDKTLNLPLAQQTISIIEILKEKTAGNLTE
                     DEENLLGTVLYDLRMKYVQSACS"
     gene            complement(6621..7982)
                     /locus_tag="PK125_05870"
     CDS             complement(6621..7982)
                     /locus_tag="PK125_05870"
                     /inference="COORDINATES: protein motif:HMM:NF012318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HPB37669.1"
                     /translation="MKKKSKNISFLLLVACGLLSLVLLSPFFLYAGKEDKIAEVVEMT
                     SKAIVNIKTEEWSKDIDEKKKPNVFRKLIVGDEEEDDDDEMFENIGSGVVLDPKGIIV
                     TNEHLVSKAINIRVKFINGKEYEAHVLGSDPEFDIALLKVNDKVDLPFLKITKSKNVR
                     VGQKVIVIGNPYGLSSSVSVGVVSALGRNLRIDNKIYANLIQTDAAINPGNSGGALLD
                     ADGNPLGIVTAIYGEGKGIGFAIPIDDVLVMLEDFLQSTVKRPIIGFFIERRRDEKGP
                     YLYINKVLNGSPAEKYGIRIGDRITELNRKKIREGMKIQSVFRTLRQQESIQLKVAKG
                     AKSVIMNIDVKDLADYVPAPMDEGLCSVKISDIKGYPKLKFKLKEKDGVVVTKVISGS
                     PGPRCGLRIGDVVFKINNNVISNKKDFDAFMAEGLKRNYILYQVKRRDEIFFLPVKLD
                     TLL"
     gene            complement(8078..9322)
                     /gene="coaBC"
                     /locus_tag="PK125_05875"
     CDS             complement(8078..9322)
                     /gene="coaBC"
                     /locus_tag="PK125_05875"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015939 - pantothenate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HPB37670.1"
                     /translation="MDKQYTMLKNKEIIVGITGGIAAYKTAELVRELSKRGANVHVVM
                     TKNAMAFVTPLTFQTLSGNPVVHEMFELFAGSKIGHIALSDIADQMVIAPATANIIGK
                     IANGIADDFLTTMVMATTVPILFVPSMNTKMWGSPMVEANIMKLKEAGYEVMEPESGD
                     LACGTSGKGRMPRIEDVLEKMEDIFTEKDLINERVLVTAGPTTEYIDPVRCITNKSSG
                     KMGYSIAKIAKRRGADVILITGKTYVAPPRTDMRIIEVETACEMRDAVMEHHRYCSII
                     IKAAAVADFKCKNETCQKMKKGDDNIVLLELEKNPDIIGELGKIKEQRILIGFAAETQ
                     NLFDHAAEKLKKKNLDIIVANDVSRSDIGFGSDSNEVTIIEATGGAKHVPMLSKDEIA
                     HIILDAAKKALKKKKRVEEDWY"
     gene            complement(9343..10530)
                     /locus_tag="PK125_05880"
     CDS             complement(9343..10530)
                     /locus_tag="PK125_05880"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HPB37671.1"
                     /translation="MKNLFILSLILFLVIPFADAEGAKKIHSNQKASSPQTSTAKEEP
                     FKSYIVMEAMSGKVIEGENIHLKRPPASVVKLMVALVVAEKVAKGEVKLTDQVTASRE
                     ASKMGGSQVYLKEGEVFTLEELMKATLVASGNDAAYAVAEHIAGSTEEFVRLMNEKAK
                     SLNMADSEFHSVHGLPPSIGQEPDLSSCSDLATLGRELLKYPKIMEWTSIKSEPFRDG
                     KFIMNNHNKLLTRMSGTDGFKTGYYRETGFNLVATAQKNGLRFIVVVLGSPTGKIRDD
                     VTVEKFKKAFSRYKMLNVVKQGEVIDKDIILSDGKYRKIKGVAGSSFQYPIANDKKGT
                     IQKEPSVPPSIKGEIREGQKLGELVVKFDNEQIGKVDIVSPVYIPKANLFTRLIRKIG
                     LNI"
     gene            complement(10530..11279)
                     /locus_tag="PK125_05885"
     CDS             complement(10530..11279)
                     /locus_tag="PK125_05885"
                     /inference="COORDINATES: protein motif:HMM:NF013324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="slipin family protein"
                     /protein_id="HPB37672.1"
                     /translation="MYIPYLFLIVLVVFFLVSAIKILNEYERGVIFRLGRVLGTPKGP
                     GLIILIPVIDRMVKVSLRTVVLDVPPQDVITRDNISVKVNAVVYFRVIDPSKAIIAVE
                     NYLYATSQLSQTTLRSVLGQAELDDLLSHREKINETLQEILDTHTEPWGIKVSNVEVK
                     NVDLPQEMQRAIARQAEAERERRAKVISAEGEFQAATKLAQASDILSKNPMSLQLRYL
                     QTLIEISSEKNSTVIFPVPIDLITAFVDKLK"
     gene            complement(11284..12567)
                     /locus_tag="PK125_05890"
     CDS             complement(11284..12567)
                     /locus_tag="PK125_05890"
                     /inference="COORDINATES: protein motif:HMM:NF014062.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nodulation protein NfeD"
                     /protein_id="HPB37673.1"
                     /translation="MNRFFAKSLLIVLLFLIPIVADANEIYTIRIHGAINPPIASFIN
                     DSINKAEKNGSEALLILMDTPGGLDTSMRDIIKGIMESNIPVIVYVYPSGARAASAGS
                     VILLASHIAAMAPGTNAGAAHPVSVGQEKADKVMMEKVAKDAEAYVKSIAKQRGRNED
                     WAAKSVRESASITATEAVEKKVADLVAGSIDELLEKIDGKTVETKKGSVVINTKGKKS
                     AEVTMPFKYKFLSYISDPNVAYILMMIGMYGILFEIYSPGAIFPGVVGGICLILALYS
                     FQTLPISYAGLFLILLGVIFFILELKIVSHGALGIAGIISLVIGSIMLIDLPSSILSI
                     SYTTIIIVAAVTGIFFFGVLSYAIKAQFSKVTTGREGLVGVEGVAQTDIFHDGKVFIH
                     GEIWNGKSDEQIPKGEKIIVTEVEGLIVKVKKKGG"
     gene            complement(12572..13057)
                     /gene="coaD"
                     /locus_tag="PK125_05895"
     CDS             complement(12572..13057)
                     /gene="coaD"
                     /locus_tag="PK125_05895"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HPB37674.1"
                     /translation="MRKRLAVYPGSFDPITNGHLDILTRGLELFDTVIVAIAHNIEKK
                     ALFTIEEKKDMIMRSVKDHLRVKVDSFEGLLVDYVKRVDAKTVIRGLRAMSDFEYEFQ
                     MASMNRELNRGMDTIFMMTSKDYFFISSRTIKEVASFGGSVAGLVPDIVQQKLKEKFK
                     G"
     gene            complement(13080..13670)
                     /gene="rsmD"
                     /locus_tag="PK125_05900"
     CDS             complement(13080..13670)
                     /gene="rsmD"
                     /locus_tag="PK125_05900"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: protein motif:HMM:TIGR00095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HPB37675.1"
                     /translation="MNSRRSFKRKFSSLRITGGFLKGRNIVLLQKSGVRYTSAKVREA
                     IFNILGDMKEKSVLDLYAGSGSFTIEALSRGAMIATSVEFDRDMAEILKKNIIALNLK
                     NSCHILTMDVAFAVPFLAKRAFSYDIIFMDPPYDKGYVGESMSLLMANRIYHTGSVIV
                     IEHSKRETPVFAEAKGFYRMDTKGYGDTFITLFQAD"
     gene            complement(13699..14640)
                     /locus_tag="PK125_05905"
     CDS             complement(13699..14640)
                     /locus_tag="PK125_05905"
                     /inference="COORDINATES: protein motif:HMM:NF014433.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsA family ATPase"
                     /protein_id="HPB37676.1"
                     /translation="MNITGKDVKLIMVGGKGGVGKTTCASAVALKLAMDGRRVLVISS
                     DPTPSLSDIFEVSIGSEEVKISDRYELYGLEISSDVVLAKWKERFGDEIYEVISSFAN
                     VDYDFVDYVGTAPGIEEEYMLSFIIELVESGKYDIVVWDTAPAGHTLRLLRLPHLFLK
                     HMEAATKFYMNMYAYLERLKDAVRFKASKRSLLEIIGSWEALSERIVEFIRDEKLTKY
                     LIITIPEALGVKLTERVIAEFKENMLKVENIVVNYVVKEADCEFHKMRKAMQQHYLDL
                     LANTYKDTNLVILNLTPWEVKGMERIAGISQALFLTP"
     gene            15026..15832
                     /locus_tag="PK125_05910"
     CDS             15026..15832
                     /locus_tag="PK125_05910"
                     /inference="COORDINATES: protein motif:HMM:TIGR00010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HPB37677.1"
                     /translation="MFVDSHCHLEMEDFQKDRKEVIERSLRQGLQYMLTVGTDETYFK
                     KVVEIINSYPAVYGAIGIHPHNSKDYNPQLEKTIAAFLKDKRIVAYGEIGLDFFKNYS
                     PRESQIHAFQLQLEVAKRSRLPVIIHSRNAKDETIQILSTDYDGNLGGVIHCYSYDLA
                     AAKKFLDMGFTLSIPGTITYKNSHSLTDVVKFTPIDKLLAETDAPFLAPHPHRGKRNE
                     PSFVRLTIEKIAQIKGKGVEEIAMNLTRNFEKLFLHEDRGEGAEEPAGQG"
     gene            15798..16499
                     /locus_tag="PK125_05915"
     CDS             15798..16499
                     /locus_tag="PK125_05915"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family cysteine hydrolase"
                     /protein_id="HPB37678.1"
                     /translation="MKEQRSQQVRDEGNKEKHEITCRGKSNNITGNNDSKRGRTLKPA
                     IIVVDMLLDNLGLERKGKGEEEKIIKPVAAFLKECRQLSVPVIFACDSFLKDDFIFQG
                     RMKPHAIRGTDGTKPLPDLEPQPSDIILEKRRFSAFFKTDLDQTLRTMHVDTIAVGGI
                     NTHFCVLATAFDAVCHDFTTIILEDLSAAYKREIHQSFIDTYRYSAIYPILRVMKSKE
                     FLSEYRDLRDLGIGD"
     gene            16505..17071
                     /locus_tag="PK125_05920"
     CDS             16505..17071
                     /locus_tag="PK125_05920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="GerMN domain-containing protein"
                     /protein_id="HPB37679.1"
                     /translation="MVTKDLRRLRGYGSAKRVSYKRLAIISLPLLVATFVFVYYFRYM
                     GNIQPQRQVIQVEKNTVSLIYTDNNGKLAEDILEIENTATQKDKGDFIINKLKAKGLV
                     PGDVSLQEMIIDSEGIIYLNFTKNLLQGIPGKDDIELTYAIVNSFLATFRHTNKVFIL
                     IDWKPVYTLRGVVYTYLPIEFNKQVMED"
     gene            17072..18346
                     /gene="serS"
                     /locus_tag="PK125_05925"
     CDS             17072..18346
                     /gene="serS"
                     /locus_tag="PK125_05925"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003218092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HPB37680.1"
                     /translation="MIDPKMLREKSEIVFESLKKRGAEINMDRLFSFDEKRRMLIQES
                     EKMKSERNELSNEIARLKKSGSDAENKISHLRDLGKDIEGLEQELRIVEKEWENMLLV
                     IPNIPHESVPVGTSETDNKEVRVWGEKPSFDFAPLPHWDIGEKLDVLDFKRAAKITGS
                     RFTLYKAGGALLERALINFMLDLHTREHGYTEVLPPFMVNRDAMTGTGQLPKFEEELF
                     KLQGIDYFLIPTAEVPVTNIYNNEILKEDDLTIKFVAYTPCFRAEAGAHGKDTRGLTR
                     QHQFNKVELVKFARPEQSYEELEGLLLDAEDVLKKLNIHYRVSLLCTGDLGFSSAKTY
                     DIEVWLPGQNIYREISSCSNFENFQARRARIRYRKSGNKIDFVHTLNGSGLAVGRTVM
                     AVMENYQNSDGSITIPEALRPYMHGITRIAAP"
     gene            complement(18512..19414)
                     /gene="secF"
                     /locus_tag="PK125_05930"
     CDS             complement(18512..19414)
                     /gene="secF"
                     /locus_tag="PK125_05930"
                     /inference="COORDINATES: protein motif:HMM:TIGR00966.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HPB37681.1"
                     /translation="MFELLKDTKVDFIGFRKKAFIISAIMMIVGLYAFVMIVMGRANL
                     SVDFTGGANLQIRFAEQVDIGALREVLTNGGISDVQIQEVSGTKDFFIKTKLSDIEKE
                     SIEDRVSSILTNQLPGKKFEILGSNMVGPGVGKDLRNYAIIAVCLAMLGIIGYIWYRF
                     TLISGLAATIATFHDVVAVLGVFYLLDKEFNLLIITALLTIAGYSLSDTVVVFDRIRE
                     NMGSMKARDDLAQLMNRSINEVLSRTIITGISTMLALGALMLLGGEVLFDFSLALLIG
                     LVVGTYSSIFIASPLVLIWRKAAK"
     gene            complement(19424..>19822)
                     /locus_tag="PK125_05935"
     CDS             complement(19424..>19822)
                     /locus_tag="PK125_05935"
                     /inference="COORDINATES: protein motif:HMM:NF014416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HPB37682.1"
                     /translation="LFLNLIYLLGAFSALKATMTLPGIAGIALTIGMGVDSNVLIFER
                     IREELRLGKTVRAAVDAGYSKAWVAIFDSHVTTLITACVLFVFGTGPIKGFAVTLIIG
                     MVINLFTAVFGSKAIFDWILQKFKPRTLSI"
BASE COUNT         5519 a         4793 c         4009 g         5501 t
ORIGIN      
        1 ttggaaacct taatttctgc tgctaccttt tctacgattt ctgcctttgt cattcttgtt
       61 ccctccttag atatgaatgt gtatttatct ataattatca ggcgtatacg taatgcctgt
      121 tgtgcaatcc cccgtagaaa caagcatcta caaaatagta ttcgcttaat tcctttaaaa
      181 atcaagcttt ttttaaattt aattacaaaa ataatctata gaggacgttg gagggaatta
      241 ttgaaggaat ctttggctac tcagtcggac acgtacctgc aaagcaggca gcagcgacac
      301 aagttccggt gaaagattaa tgttgttaga ttgattgaca tggcactgaa tcacccattc
      361 tctcaatcac atcttccagg gagcccgcaa atggcccaac agactccatc ttgatcgatg
      421 caagggctcc agcgaactga agagagtctt caacaggata atcgaacctg cgcgctatat
      481 aagatccgat gaccgtgtcg ccgcgcccca tcctgccttt agtgctcctg tttgaaaatt
      541 ttgcatattt tgttgttccg tggcttcgcg ccagtacacc acctgaagac gtaataatag
      601 tctcagggct tccccagcct gccagtatgg cagcctggtc ttgcaagacg tcggtgcctg
      661 tcaaagcctg tgcctcctta ctgtcaagct ttacgaaatc tgccattctc aggatttctt
      721 ttttctctgg aacatcttca aggcggacaa tgccggtctc atggtctgcc tgcagcatga
      781 gaccctgcat gtctactgat aaacggaatc ctcttacctt tagtgaccgc atcaaatcca
      841 tctgggcctc gcgagggccc atacagcaac agtgtaacag gcatggctca aaagggggta
      901 ctttttcaat attacttccc cctttaacat gaaaagcctg cctctcatca acatttgcat
      961 ttgggaagac aacacggtat tgagcagtct ctccaggctg aatgtagaga tctataccag
     1021 cctttttcat aggctccaaa aggtattctt cctttttgga gattgtcgta accacagcaa
     1081 tcctctttcc aacacaagat gctgctattg aggcaaacag aaccgggctg cccaatataa
     1141 cgaagggaac tccttcaaaa ggaactacag tgcccatacc catctgcccc acgaacacaa
     1201 tatcatactg gttcttcttg ctcatttccc ttctactatt taaacttacc ttttaaattg
     1261 tccaaagtgt acgggattga ttcattgtac ttatcccttc tgtttccccc atattataag
     1321 cttcagcagg agggaaacac aacagacatg atagaatcta tttttttcgt agtcatcgat
     1381 tgtgtcattg aacaagatta aaacttgtat atgtgttttg ccatttgtgc tgttgtgaaa
     1441 gtaggctaaa gatggtaaaa aatagtgtgg caaagaccgg ttcataagga agactgagag
     1501 taatggaata caagatctca aagggtttcg ttgtgaaagc aggggtgcgc ttgatgatgt
     1561 taatcctcgc tacaaacctg ttcctccatg gtgtactact ttttgccgct gaaaaatcat
     1621 cacagaaagg agaaaagaag atgagcgata cacaactgaa agccacaatt catacaacaa
     1681 aaggagatat cagacttaat ctttttcctg gcaaagcacc tctgacggtt ctcaattttg
     1741 taaatcttgc caaaagaggg ttttacaatg gtctcagctt ccacagggta attccagatt
     1801 tcatgattca gggtggatgt ccgctcggca ctggaacagg aggtcctggc tatcggttta
     1861 ctgatgagtt ttcgtctgac ctcaagcaca gcaaggccgg cattctttca atggccaatg
     1921 cagggccaaa tacaaacgga agtcagtttt tcattaccca tgtgcccact ccgtggctgg
     1981 acaacaaaca cgccatattt ggagaggtag tcgatgaaaa agaccagaca gtggtaaaca
     2041 gcatcgtaaa gggggacaag atcacgtcta tttccatcga tggagattat tcaatacttg
     2101 cagaccagaa taaggatacc cttaataagt ggaacgaagt attggagaaa caagccagat
     2161 aaaaccggtt tttcgcgtct caatctgaac atgacccgca cgaagaaata ctggtggttc
     2221 cgccagtggg gcagccggat tcacatccct ttgttcatag acacgtaagc gattcaccgt
     2281 gcttcttctg gccgttttac aaagcccatt aggacagccg caattactga tgccacgacc
     2341 gcaatgatga atgggatcga gaaagagcca gttgcatctc taatatggcc tgcaaatcgg
     2401 tgagcaagga ttgcgccggt cccgtaaaag attgtaaggg ccccgataac cgtacccatg
     2461 atctccttcc gaaagtaatc acccccggca gcgccataca tgggaaacgt tgctccaaaa
     2521 aaagcgccaa gagcgcctac acttgtaaag agccacacca cattgccccc tgatgcaata
     2581 atgcaaacaa tagctacgcc aacacaaaaa ttggaaaaga gtattgtcat ccgtctgccg
     2641 atatagtcag atatcatacc gattgtgagg acaccaacaa tttgtccaat accatggatt
     2701 gtggccagca atgacgcccg ctcataaggg aaaccaagct cgtaccgcgc gtaatcaacc
     2761 ataaacgttg tagggatata cagtgatccg gctacgagca aataggaaaa accaagcatc
     2821 cagaaccttc cctttgtgag gatttctgaa tacctggttt tcacagcctg gcttgtggtg
     2881 gcttgaggtg ctggtataat ctcacctgca ggggttcccc atggtacaag ccctctgtcc
     2941 tccggtttac ttctcaggag aaaaatattt actaatacca tgatcagggc tgcaatgccc
     3001 agaaaatacc agcaataacg ccagttccat tctgcaacta tgatgggaaa caatctgccc
     3061 atggaagcaa aaccgaggcc aaaaccagta gagagaatac cgagggccat tccccttctt
     3121 ttcacggcaa accatcgctg aactaacgtg ataataggcg tccacatggc agccgctccc
     3181 atacccacaa taccaaaaaa cagggatgcc tgccagaagg aagtagacgt gcccataagc
     3241 agggttccaa tgcccaggac aactccaaac atgggaatga tttttcttgc gccaagccgg
     3301 tctgtcagat tgcctgcaaa taatgaaaga ataatatacg caagaaaata ggcattgaaa
     3361 atatctcccg cctgtcgtcg tgtaaaaccc agggtacgta tcatttctgg caatacaaca
     3421 ctatatccga ggcggatacc ataatttaca aaaagattga taaagcagat aacaagaatg
     3481 acccatgccc agtgaatggc ctggtttttg tctaaagcat tgctcttcat aatatccccc
     3541 ctttttttaa aacttatcca gggttaaagg ttaaagacac gaggataaag gccggaaaac
     3601 cttctgcttt atcaatcgca ccttttacca tgaaccttga acctttaccc gtagcctcga
     3661 tcctctcgcc cgttttcatt gcatacccag cttcttcctt ttcgcttcta tatgggcaag
     3721 taccccatcg accgctttaa ttggatcttt ttccacactt aggacaccgc ctgtcacctg
     3781 gggcaggtca actgtaagca gctggacaag gtttggcgct cctgttaccg taggaacagg
     3841 attcacatag gtataaaggc caagggcgag tgcgaacaca gcatctatgg tggctttctg
     3901 ttccatatac tcaggagcaa cagctgcaac aggaaggtca ggaatcggca cctgcataac
     3961 attggcaatg gctgccagca tatctgcaag ccgtcctgta tccgtacagg ttccgtagct
     4021 gagcacagga ggaatcttca gggtttcgca taatgcccgc aggccattcc ctgcaaaatc
     4081 ctttgcctca gggctgcaga gcccggcaac ctgcattgca gcattcccgc atcccataga
     4141 gaggacaagg atatcccgct tgataagttc ttttgctata gtcacgctat ggacatcctg
     4201 ccccgtgtca cgcaacgatg tgcacgagac aagacctgcc acccccctta taaccccatc
     4261 ctgtattgcc ttcaaaaggg gcaaaaccga accacccagg gcagctgcga tactctcagt
     4321 agaaaaaccg actattgcct cccccatatt cagccctgta atcggcttta ttgtggcatg
     4381 tcgctcttta aaattgtcaa tggccatctg taggagtgag gctgcttgct ccgaagcttt
     4441 ttccgggacg tagtcaatcc gatcagttat gccttcaaag gcaacaagct ggctcacagg
     4501 gatcagcttg aatttatatt tttctgcata gaaaggatcc acaggcatcg aacaattcat
     4561 atcacaggca aaaagatcga tgcaaccact tgccaggata gcctcctgca taatccaatt
     4621 tcctgtaaac ccatagaaaa catcgtccat ctgccaacgt tggatcatct cctgaccggt
     4681 ttcaatattt gcaataacac gcacaccctt tgcaccaact gcttttgcct tttcctgcca
     4741 ctcatttttt cgcgcaagct ctaccatggt gaagccgaga aaaggctcat gaccattggg
     4801 aagaacattg acatattcag ggtcaagcac gccaagatca acccgcatag ggtgaggtct
     4861 gggaatcccg aagaggatat cctggcagaa ctcattcaca atctggctct ggtacgccat
     4921 cgcaatccca agtcgcatag ctttcaaggc caggctggca taataaccat caacgttggt
     4981 aagacatgag ctcgtggcaa acatcatctc gccgtaaatc cccccgggaa atatacccaa
     5041 ctttttccac accgcttttc gttccttggg ggcaagtgtc tccacaatat aactcggctc
     5101 atcatatttc ctgttaaaat cagcttccac ataattacag agtgccaggg caacttcttc
     5161 gtcggaaagc gatgttggaa tgtcaaaccg ctcagcaaat gcaataagct tccccggttc
     5221 cgttatggca aaaggggctt tgccgtttac agctgctttc aacgttttaa tggtttgctc
     5281 agtgtgatag tggtatgttg atgctcccat aacattcctc aggagcatca tcctcatagc
     5341 cattccatca ccggttatgc cgcacacacc tctcttgtct ttggccacat cagccctgca
     5401 cggcccgttg gagcaaaggt cgcaccgaac acctccctgg cagaaagaac atcgctggtc
     5461 agggttaccc cccatgccct gggcttcata gcgatcccac atgtttgtca ttccatcctg
     5521 cttaatccgc tgatacatgg tacgaattga ctcatggtag gaaattctct catcttccat
     5581 agcccagtcc ctcctgacgt aaaattataa atgtatagat attattttag tgataaccgg
     5641 aacaataaac aaggcaaaac atggagtaga acgaaaaggt actgggctga aatgtcaggc
     5701 tttaactttg tgtgaatctg cagaggtgat agaacttcat gcaggcctct tctacgctgg
     5761 ctataatcct gtcataggcc ttaaggaggg ttccattatc atactttaat atattttttg
     5821 tctctgcaaa gattacgtca taatcgtgtc ttaatgacac acagtcccat aacgtgcagt
     5881 tctcaacact gagggaatac tgcctctccc agctgtcgag cattgcatcc tcaaagcgaa
     5941 cctcgaggag cttcacctcc tcgtgattgt ttaatagctg ctggaaccgt tctgctgatc
     6001 tcgcctctgg atggagataa tagtgggctg tatatccttt gggtgagaac cagaggagtg
     6061 gatgaaacga gtcttctatt gtgtgatagc cacgagtgat gagcctgaca tccgccattg
     6121 gctttatgaa attgcgacat tcaggcaaaa tctcgtaaag ctccatgcat gctttatcgc
     6181 aaaatatcta ttaatcttta gcgatattca ctccggaaaa ctgagtcgat ttctcaacta
     6241 caagctgatt gaacatattt cattcgaagg tcatagagaa ccgttccaag caggttctcc
     6301 tcgtcctctg tgaggttgcc tgcagttttt tctttgagta tttctatgat gcttattgtc
     6361 tgttgcgcga gaggaagatt cagtgtttta tcctttgtaa tcgggtcagg caattccccg
     6421 aggcaaacaa gggcggatgt tgttagagac agtataaatg tagaaaagtt aaggacagtg
     6481 gtaaaggcct cctcctcggc aatctcagca caggttcttt catcttgctt cttttctgga
     6541 tctactgcct ctgcctcgct ggcactcgct cttttatctg tgatcttgaa gcctttctga
     6601 tcatcatcca tatcagcctc ctaaagtagc gtgtcgagtt taacaggcaa aaagaaaatc
     6661 tcatcacgtc ttttcacctg ataaagaata taatttcttt ttagcccttc tgccataaat
     6721 gcgtcgaaat cttttttgtt tgaaatgacg ttgttgttga tcttaaagac tacatctcct
     6781 atccgcaatc cgcatctggg tccgggtgat ccgctgatga ccttggttac aacaactccg
     6841 tccttctctt taagtttgaa cttcaacttt ggataacctt taatatccga tatttttaca
     6901 ctacagagcc cttcatccat gggggctgga acataatcgg ccaggtcttt aacatcaata
     6961 ttcatgatca cagatttcgc cccttttgct actttgagct ggattgattc ctgttgtctc
     7021 agcgtcctga atacactttg tatcttcatt ccctccctta tcttcttcct gttaagttct
     7081 gtaattctgt caccaatcct gataccgtac ttttctgctg ggcttccgtt cagtaccttg
     7141 ttaatataga gataaggtcc tttttcgtcc ctgcgccttt caatgaaaaa tccgataata
     7201 ggtctcttca cagtgctctg taaaaaatct tcaagcataa caagcacatc atcaatggga
     7261 attgcaaagc ctattccttt accctctcca tatatagcag taactatacc aagaggattt
     7321 ccatcagcat caaggagggc cccaccgctg ttccccggat taatggcagc atcggtttgg
     7381 atcaggtttg cgtaaatctt gttatcaatc ctcaggttcc tcccaagggc gcttactaca
     7441 cctacgctta ccgaacttga caggccatac ggattaccta tgacaatcac cttttgtcct
     7501 accctcacat tctttgactt tgtaatcttc aagaagggca ggtcaacttt atcattcacc
     7561 ttcagtaatg caatatcaaa ttccgggtcg ctaccgagga cgtgtgcctc atattccttg
     7621 ccgtttataa acttaacccg aatattgatc gccttcgaaa ccagatgttc attcgtcact
     7681 ataatgccct ttggatccag aactacaccg gatcctatgt tctcgaacat ctcatcatcg
     7741 tcatcttctt cttcatcacc aacaatgagt ttcctgaata catttggttt ctttttttca
     7801 tctatatcct ttgaccactc ttcagttttt atattgacaa ttgctttgct cgtcatctcg
     7861 acaacttcgg ctatcttgtc ttcctttccg gcatacagaa aaaacgggga tagcaagacc
     7921 agagatagca atccgcaagc aacgagcagt aagaaggaaa tatttttaga cttttttttc
     7981 atagcacaga atgtattgaa ccgaaaaact tgcgatatga aacaaattta ctgtgatttt
     8041 cactataatc tatgagctat cagctgtgaa ctgcctttca ataccagtct tcttctactc
     8101 tttttttctt ttttaatgct tttttcgctg catcgagaat gatgtgggca atctcatcct
     8161 tactcaacat gggtacatgt tttgcgccac ctgttgcctc aattatggtg acctcgttgc
     8221 tgtctgaacc aaacccaata tccgaccttg atacatcatt agcaacaatt atgtccaggt
     8281 tttttttctt cagtttttct gctgcatggt caaataggtt ctgtgtctct gcagcaaaac
     8341 cgatgagaat cctctgttcc ttaatcttac caagttcacc gataatatca ggattttttt
     8401 caagttcaag caaaacaatg ttgtcatcgc ccttcttcat tttttgacag gtttcgtttt
     8461 tacatttgaa atcagcaacg gctgctgctt ttataattat cgaacagtat ctatggtgct
     8521 ccatcacagc gtcccgcatc tcacatgcag tctcaacttc aatgatgcgc atatctgttc
     8581 tgggcggtgc tacataggtt ttacctgtaa tcaaaataac atctgcccct cttctctttg
     8641 caatctttgc tatagaataa cccatcttac cggaagactt gtttgtgata caccttaccg
     8701 gatcgatata ttctgttgta gggccggcag tgaccagtac ccgttcattt ataaggtctt
     8761 tttcagtaaa aatgtcttcc attttctcga ggacatcttc tatccggggc attcttccct
     8821 tacctgacgt gccgcaggcg agatccccgc tttcgggctc cataacttcg tatcccgcct
     8881 ccttgagttt cattatattt gcttctacca tgggactacc ccacatcttt gtgttcatgg
     8941 atggcacaaa aagaatgggg acagtggtgg ccatgaccat ggtcgtcagg aaatcgtctg
     9001 ctatgccatt ggcaattttt ccaatgatat tggctgtagc cggtgctatt accatctgat
     9061 cagcaatatc agaaagtgcg atatgaccta tcttggagcc tgcgaaaagc tcgaacattt
     9121 catgaaccac cggatttccc gaaagagtct gaaatgtgag aggagtaaca aacgccatgg
     9181 cattctttgt cataacaacg tggacatttg cccctctttt tgagagttcc cgcaccagtt
     9241 cagctgtctt gtaagcagca atacctcctg taatacccac aatgatctct ttgttcttca
     9301 gcatcgtata ttgcttgtcc atatctccca ttaaatctct catcaaatgt ttaggccaat
     9361 ctttcttatc agccgcgtaa ataggtttgc ctttggaata taaactggcg acacaatatc
     9421 aaccttccct atctgttcgt tgtcgaactt cacaacaagt tcaccgagct tttgaccttc
     9481 cctgatttcc cctttaatgc tgggcgggac agaaggttct ttttgaatgg tccctttttt
     9541 atcattcgca atggggtact gaaagctgga tccagcaaca ccctttatct tcctgtactt
     9601 tccgtcactg agaataatat ctttatcaat cacctcaccc tgtttaacca cattcagcat
     9661 tttataccgg ctaaatgctt ttttaaactt ttccaccgtt acgtcatctc ttattttccc
     9721 tgtcgggctt ccaaggacaa ctacgataaa tctcaagcca tttttctggg ctgtggcaac
     9781 gagattaaaa cctgtttcac ggtaataccc ggttttaaaa ccatctgttc cactcatacg
     9841 agtaagcagt ttgttatggt tgttcataat aaacttgccg tctctgaagg gttcactttt
     9901 aatcgatgtc cattccatga ttttgggata cttcaagagc tcccgtccaa gcgttgcaag
     9961 gtcactgcat gaacttaaat ccggttcctg ccctatagaa ggtggtagtc cgtggacaga
    10021 atggaattct gaatctgcca tattcagaga ttttgccttt tcattcatga gcctgacaaa
    10081 ttcctctgtg ctcccggcaa tgtgttctgc aacagcatag gcagcgtcat tccctgacgc
    10141 aaccagggtg gccttcatta gttcttccag ggtgaatacc tctccctctt tcaggtaaac
    10201 ctggctacca cccatcttag aggcttcccg cgaggctgtt acctgatcgg tcaattttac
    10261 ctcgcccttt gcgactttct ctgcaaccac aagggcaacc ataagcttca caacacttgc
    10321 gggtggcctt ttcaggtgaa tattctcacc ctcaatgact ttgccgctca tggcttccat
    10381 tacaatgtat gacttgaaag gttcttcctt cgcagttgaa gtctgaggtg acgacgcttt
    10441 ctgattgctg tggatctttt ttgccccttc ggcgtcagcg aatgggatga ctaggaaaag
    10501 aatcagggac aggatgaaca ggtttttcat tatttcaact tatccacaaa agctgtgatg
    10561 agatctatgg gaaccgggaa aatcacagta gaattctttt ctgaggatat ctcgattagt
    10621 gtctggagat atcgaagctg aagcgacatt ggatttttgg aaaggatgtc ggatgcctga
    10681 gccaactttg tagctgcctg aaattctcct tctgctgaga ttacttttgc ccttctctcc
    10741 ctctcagcct ctgcctgtcg tgcaatggcc cgctgcatct cctgcggaag gtcaacgttc
    10801 ttgacctcca cattcgacac cttgattccc cacggttctg tatgggtgtc gagtatctcc
    10861 tgaagtgttt cattgatctt ttcccggtga gaaagaaggt catcaagctc tgcctgaccc
    10921 agaacactcc gcagggttgt ctgagaaagc tggcttgtgg cataaagata gttttcaact
    10981 gcaataatag cctttgatgg gtctatgacc ctgaaataga caacagcatt caccttgact
    11041 gaaatattat ccctggtgat aacatcctgg ggtggtacat ccagtactac ggtacgcagg
    11101 cttaccttga ccatcctatc gataacagga ataagaatga taagcccggg ccctttcgga
    11161 gtgccaagaa cccttcctag cctgaatatg acgcctcttt cgtattcgtt caggatcttg
    11221 atagcactta caaggaaaaa tactacgagc actatgagaa acagatatgg aatatacatg
    11281 attttatccc ccttttttct tcacttttac tatgagccct tccacttcag tcacaatgat
    11341 tttctcccct ttaggaatct gttcgtcgct ctttccgttc caaatctctc catggataaa
    11401 aaccttacca tcgtgaaaaa tatctgtctg tgcaacacct tccaccccaa ccaagccttc
    11461 tcttcctgtt gtaaccttgg aaaactgcgc ttttattgca taagatagca caccgaaaaa
    11521 aaatatacca gttacagctg ccactataat gatcgtagtg tatgagatgg aaagaatgct
    11581 cgatggaaga tcgataagca taatagagcc tatcacaagg gagataattc ctgcaattcc
    11641 aagggcccca tgacttacaa tctttaactc taaaatgaaa aatatgacac cgagaagtat
    11701 taagaagagg cctgcatagc tgatgggaag agtctggaaa gaatagaggg caagtataag
    11761 acatatgcca ccaacgacac ctggaaaaat agcacctggg ctgtaaattt cgaacagaat
    11821 tccgtacatt ccaatcatca taaggatata cgccacattg gggtcgctga tatacgagag
    11881 gaacttgtac ttaaaaggca tggtaacctc agcagatttc ttgccttttg tattgatgac
    11941 gactgagcct ttctttgttt ccactgtttt gccatcgatc ttttcaagaa gctcgtctat
    12001 actgccagcg acaagatctg ccaccttctt ctccacagct tcagttgcag ttatagaagc
    12061 actctctctt actgattttg cggcccagtc ttcattccta ccccgttgtt tggctatact
    12121 tttaacataa gcctccgcgt ccttagctac tttttccatc attactttgt ctgccttttc
    12181 ctgaccgacg cttacaggat gagccgctcc tgcatttgta cccggagcca tggcagcgat
    12241 atgagatgcg agaagaatta cactgcctgc tgatgctgcc cgtgcgcctg aaggataaac
    12301 atagacaata actggaatat tcgactccat aatccccttg attatatccc gcattgaagt
    12361 atcgaggcca ccgggcgtgt ccataaggat aaggagtgcc tctgatccat tcttttccgc
    12421 tttattaatt gagtcattaa taaaacttgc tattggggga ttaatggctc cgtgaatcct
    12481 gatagtatag atttcatttg catctgcaac aatgggtatc aggaaaagta ggacaataag
    12541 cagggatttt gcaaaaaacc tgttcatccc ttcacccctt aaatttttct ttcagttttt
    12601 gctgaacaat atctgggacg agaccggcaa cggagccgcc aaagctggcg acctctttaa
    12661 tcgtccgtga gctaataaag aaatagtctt tgcttgtcat catgaagata gtgtccatcc
    12721 ctctgttcaa ctccctgttc atagaggcca tctggaactc atactcaaaa tcactcatcg
    12781 ctctgagccc gcgaatgact gtcttggcat caaccctctt gacataatct accagcaggc
    12841 cctcaaagct gtccaccttt accctgaggt gatcttttac cgatcgcatg atcatgtcct
    12901 ttttttcttc aatggtaaaa agggcctttt tttcaatatt gtgggcaata gctacaataa
    12961 ctgtgtcaaa cagctcaagc cctcttgtca atatatccag gtgcccgttt gtaataggat
    13021 caaaagaccc aggataaaca gccaatcgtt ttctcatgaa cctctccttt taacgtaatt
    13081 taatcagcct ggaacagagt aatgaatgtg tctccgtaac cttttgtgtc catccgataa
    13141 aaccccttag cctcagcaaa aacaggtgtc tctctctttg aatgctctat tacaatgacg
    13201 gaaccagtgt ggtaaatcct attcgccata agcaggctca tcgattcccc aacatacccc
    13261 ttatcataag gggggtccat aaagattata tcataggaaa aagccctttt ggcaagaaat
    13321 ggaactgcaa atgctacatc catggtgaga atatggcaag agttttttag attcagggct
    13381 ataatgtttt tcttgagtat ttctgccatg tctctgtcaa attccactga agttgctatc
    13441 atcgcacctc tgctcagtgc ctcaatagtg aatgagcctg acccggcata gaggtcaagc
    13501 acactttttt ctttcatatc cccaagaata ttaaagatag cttccctcac ctttgccgaa
    13561 gtataccgta caccactctt ctggagaagg acgatgttcc ttcctttcag aaaaccaccg
    13621 gttatgcgca aggaactgaa cttcctcttg aatgatcttc tgctgttcac gtcataatga
    13681 ttcacaaaca gtatcacact acggggttag aaataatgcc tgggagatcc ctgcaatgcg
    13741 ctccatgccc ttcacttccc agggagtgag attgaggata actaaattcg tatccttata
    13801 agtattggcg agaaggtcaa gataatgttg ctgcatcgcc tttctcattt tgtggaattc
    13861 gcagtctgcc tcctttacaa cataatttac cactatattt tcaaccttga gcatattctc
    13921 tttaaactca gcaatgaccc tttctgtcaa ttttacccca agagcttcag ggattgtgat
    13981 aatgagatat ttcgtcagtt tttcatccct gataaattca acgattctct ctgaaagggc
    14041 ttcccaactc ccaatgatct ccagaagaga ccgtttggaa gctttaaatc taactgcgtc
    14101 ctttagtctc tccagataag catacatatt catataaaac ttcgttgcag cttccatatg
    14161 tttcaaaaaa agatggggta accgcaggag tctgagggtg tgcccggcag gggccgtatc
    14221 ccacacaact atatcgtatt ttccgctttc aacaagctct ataataaagc tgagcatata
    14281 ttcctcttct ataccggggg ctgttcccac ataatctaca aaatcatagt caacgtttgc
    14341 gaaggacgag attacctcat agatctcatc tccaaacctt tctttccatt ttgcaagaac
    14401 aacatccgat gagatctcca gcccatagag ttcatacctg tcagaaatct ttacctcctc
    14461 gctgccaata gatacttcaa agatatctga cagggacggc gtcggatcgc tcgaaatcac
    14521 aagcaccctc cttccatcca tggccagttt cagtgccaca gcagaagcac aggtggtttt
    14581 gcctacacct cctttccccc caaccataat gagttttaca tccttgcctg tgatgttcac
    14641 aagacccaag agagccctcc taccaagtct aacctttcgg aacttgtcat tatgtgtctt
    14701 acaggcgtat tataatatga attgtggctg tggaaaagga aaaagctatg ccttgacctg
    14761 cctcacaaat gatattctgg aatgtctggc ccaatttggc aaacagaatt tctccggtgt
    14821 ttttaatctg tgcctgttat ctttgcagcc cagctttagg ttatccgttc catattcttc
    14881 gttggtacag tgtggcacat ttttaagaaa cagtgataga ggctggtggc cctgagccaa
    14941 aagaacagat cagcgcccgg ggactccaag aataaaacgt gggtgcactg tggcatcctc
    15001 ttactcaaat atggtataag tcttcatgtt tgttgattct cattgccatc tcgagatgga
    15061 agactttcaa aaagatcgga aagaagtcat tgaaagaagt ctcaggcaag gtctgcagta
    15121 tatgctcacc gtgggcacag atgagaccta ttttaaaaag gttgttgaaa tcatcaacag
    15181 ctaccctgct gtttacggag caatcggtat tcacccgcat aacagcaaag actacaaccc
    15241 acagcttgaa aaaacaatag ccgccttttt gaaagataaa agaattgtgg cttatgggga
    15301 gatagggctt gatttcttca agaattattc tccgcgagaa tctcagatcc atgcttttca
    15361 actccagtta gaggttgcca agagatccag gctcccggta atcatccatt cacgtaacgc
    15421 aaaggatgaa actatccaga ttctcagtac agattatgat ggaaacctgg gcggtgtaat
    15481 ccactgctat tcctacgatc ttgctgctgc gaagaaattc ctcgacatgg gcttcaccct
    15541 ttcaataccg ggcacaatta catataaaaa ctcccatagt ctcacagatg tagtcaaatt
    15601 cactccaatc gacaaactcc tcgctgaaac tgacgcacct ttcttggccc cccatcccca
    15661 caggggcaaa agaaacgagc cctcttttgt gaggttaacc attgaaaaaa ttgctcagat
    15721 aaaaggcaaa ggagtagaag aaattgccat gaacctgacg cggaactttg aaaaactctt
    15781 tctccatgaa gacaggggtg aaggagcaga ggagccagca ggtcagggat gaagggaaca
    15841 aagaaaaaca tgaaatcacg tgccgtggaa aatcgaataa catcacaggc aataatgact
    15901 caaagagagg cagaacattg aagccggcaa taattgttgt tgatatgctc ctggacaacc
    15961 tggggcttga aagaaaaggt aagggggagg aagaaaaaat cataaaacca gtagctgcgt
    16021 tccttaagga atgcagacag ttatcagttc ctgttatttt tgcctgcgac agcttcctca
    16081 aggatgattt catctttcag ggaaggatga agccccacgc catacgaggc acagacggaa
    16141 caaaacccct cccagacctt gaaccacagc catcagatat tatccttgaa aagagaagat
    16201 tcagtgcatt ctttaaaaca gacctggatc agacacttcg aaccatgcat gttgacacaa
    16261 ttgcggttgg agggataaac acccatttct gcgttctcgc aaccgctttt gatgctgtct
    16321 gccatgattt cactaccatc attcttgagg atctgagcgc tgcttataag agggagattc
    16381 atcagtcctt catcgatacc tatcggtaca gcgcaattta tccaatcctc agggttatga
    16441 agtcgaagga gttcctgtca gaatacagag atttaaggga tctcggcatt ggggattaga
    16501 tcaaatggtc accaaagatt tgaggcggct cagaggttat ggatcagcaa aacgggtaag
    16561 ctataagaga cttgccataa tcagcctgcc ccttctggtt gccacttttg ttttcgttta
    16621 ttatttccga tatatgggga atatccaacc acagagacag gttattcagg tcgaaaaaaa
    16681 tactgtttcc ctcatatata ctgacaataa tggaaaactc gccgaagata ttctcgaaat
    16741 tgaaaacaca gctacacaga aagacaaggg agattttatt atcaataaac tcaaagcaaa
    16801 aggtcttgtg cccggtgatg tatcactcca ggaaatgatt attgatagtg aaggcattat
    16861 ttatcttaac tttacaaaaa atcttcttca gggcattcct ggtaaggatg atatagagct
    16921 cacatatgcc attgtaaact cttttttagc tacttttcgc cacacaaata aagtgttcat
    16981 tcttatcgac tggaagcctg tctataccct tcggggagtt gtctatacat atttgcctat
    17041 agagttcaac aaacaagtaa tggaggatta aatgattgac ccaaaaatgt tgagggaaaa
    17101 aagcgagata gtctttgaga gcctgaaaaa aagaggcgct gaaatcaata tggacaggct
    17161 tttctcattt gatgaaaaaa gaaggatgct catccaggaa tcagagaaga tgaagagcga
    17221 acggaatgag ctttccaatg aaatagccag acttaaaaaa tcaggttctg atgcagagaa
    17281 taaaataagc cacctgaggg accttggtaa agacattgaa ggattagaac aagagctcag
    17341 gatcgttgaa aaagagtggg agaacatgct tcttgtgatt ccaaatatcc ctcatgaaag
    17401 tgtgcctgtt ggaaccagtg aaacagacaa caaagaggtg cgggtgtggg gagaaaagcc
    17461 ttcctttgat tttgcccccc tcccccactg ggatattgga gaaaaacttg atgtactcga
    17521 ctttaaaagg gcagcaaaga taaccggatc acggtttacc ctctacaaag cagggggcgc
    17581 cctccttgaa agggcattaa tcaatttcat gctcgacctg cacacgagag aacatggata
    17641 caccgaggta ctccccccct tcatggtcaa tcgtgatgcc atgacaggga ccggccaact
    17701 gccaaagttc gaggaggaac ttttcaaact tcagggcatt gattacttcc ttattcccac
    17761 tgcagaagta cccgtaacaa atatttacaa caatgagatt ctgaaagaag atgacctcac
    17821 catcaagttt gtggcatata ccccttgttt tcgtgcagaa gcaggagcac atgggaaaga
    17881 tacgagggga ctcacccggc aacaccaatt taacaaggtg gaactcgtga agttcgccag
    17941 accggagcag tcatatgagg aattggaagg actcctcctt gatgctgagg atgttctcaa
    18001 gaagcttaat atccattatc gcgtttccct cctatgcacc ggagaccttg gtttctcctc
    18061 ggcaaaaaca tacgatatcg aggtttggtt gccaggacag aacatctaca gagaaatatc
    18121 atcgtgtagc aactttgaaa acttccaggc acggagagca agaatcagat atcgcaaatc
    18181 aggaaacaag atagattttg tccatacttt gaacgggtca gggttagcag tggggcgaac
    18241 agtaatggcg gttatggaga attaccagaa ctcagatgga tcaataacta tccctgaagc
    18301 attgaggcca tatatgcatg gcattaccag gattgccgct ccttaacaaa agggcgatcg
    18361 attcacatga gcacacgcaa agatataaat gccagctgtt taatagagat gggatacttt
    18421 ctttttccca cctacctgaa aaataaactg gtaaaacatg caaaacgaat ggtttcagag
    18481 tttgaagcgt gttattactt tccgattcga tctactttgc agcttttctc caaattagga
    18541 ccagcgggct cgcaataaaa atggatgagt aggttcctac aaccagacca ataagcagtg
    18601 caagtgagaa gtcaaacaac acctctccgc caagcagcat aagggcgccc agcgccaaca
    18661 tagtgctgat gcctgttata attgtcctgc tgagaacctc gttgatactt ctgttcatca
    18721 actgggcaag gtcatccctt gctttcatgc ttcccatgtt ctcccggatc cggtcaaaga
    18781 caaccactgt atctgaaagc gagtacccgg ctatggttaa aagggctgta ataatgagaa
    18841 ggttgaattc tttgtcgagc aaataaaaaa cgccaagcac agccaccaca tcgtgaaagg
    18901 tggcaatagt ggctgccaaa cccgaaataa gagtaaacct gtaccatatg tagccaatga
    18961 tgccgagcat ggccaggcat actgcgataa tagcatagtt cctcaaatct ttgcccaccc
    19021 cgggacctac catgtttgat ccaaggatct cgaatttttt tcctgggagc tgattggtta
    19081 agattgacga tactctgtcc tctatacttt ccttttcaat atcagaaagc tttgtcttaa
    19141 tgaaaaaatc ctttgttcca ctcacttcct gaatctgcac atctgagatt ccaccatttg
    19201 tgagtacctc tctcagggcc ccaatatcga cctgctctgc aaatcgaatc tgcaaattgg
    19261 cgccaccggt aaaatcgaca ctaaggttag ccctgcccat gacaatcata acaaacgcat
    19321 aaagtccaac tatcatcatt atcgccgaga taataaaagc cttctttcgg aatcctataa
    19381 agtcgacctt ggtatcttta agtaactcaa acataaatca gcctcagatg ctcagcgttc
    19441 tcggcttaaa cttctggaga atccagtcga aaatcgcttt ggaaccaaat actgctgtaa
    19501 aaaggttaat gaccataccg ataatcaggg taacagcaaa gcctttgatc ggtccggtgc
    19561 caaacacgaa gagtacgcac gcggtgataa gggtggtgac gtgagagtca aagatggcca
    19621 cccatgcctt tgaatatcct gcatccactg cagccctaac agtcttgccc agtctcaact
    19681 cctcccgtat cctctcaaaa atgagaacgt tcgagtcaac acccatgcca atggtaaggg
    19741 caataccggc aatacccggg agggtcattg tcgcttttaa ggcgctgaaa gcgccgagaa
    19801 ggtatatcag gttcagaaaa ag
//
LOCUS       DAOOMC010000043        19433 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdus sp. isolate AMR_MDS_2685
            NODE_2825_length_19433_cov_6.559552, whole genome shotgun sequence.
ACCESSION   DAOOMC010000043 DAOOMC010000000
VERSION     DAOOMC010000043.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864760
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdus sp. (wastewater metagenome)
  ORGANISM  Syntrophorhabdus sp.
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae; Syntrophorhabdus.
REFERENCE   1  (bases 1 to 19433)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19433)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,729
            Genes (coding)                    :: 2,708
            CDSs (with protein)               :: 2,708
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 7 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19433
                     /organism="Syntrophorhabdus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2825_length_19433_cov_6.559552"
                     /isolate="AMR_MDS_2685"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2699756"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(12..86)
                     /locus_tag="PK125_05940"
     tRNA            complement(12..86)
                     /locus_tag="PK125_05940"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(52..54),aa:Val,seq:gac)
     gene            complement(219..2798)
                     /gene="clpB"
                     /locus_tag="PK125_05945"
     CDS             complement(219..2798)
                     /gene="clpB"
                     /locus_tag="PK125_05945"
                     /inference="COORDINATES: protein motif:HMM:TIGR03346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HPB37683.1"
                     /translation="MNWEKLTIKAQEVISEAQKKAEASHHQGIDNEHLLYALVTQKDG
                     IVRPILDKLGANSGAILQDLEKELKNIPAVHGAAQVYITPRMKQTLDTAFIEAERLKD
                     EYISTEHLLIGVTDTGNGAAGRILQGQGVTKDRIYSVLKDIRGAQRVTDQAPEEKYQA
                     LQKYCRDLTEEARKGKLDPVIGRDEEVRRVMQVLSRRTKNNPVVIGEPGVGKTAIVEG
                     LAQRIVSGDIPETLKNKKVLSLDLGAMLAGAKYRGEFEDRLKAVLKEIDEAAGAIILF
                     IDELHTIVGAGSAQGAIDASNMLKPALARGELRCIGATTLDEYRKYVEKDAALERRFQ
                     PVYVGEPSVEETIAILRGLKEKYELHHGVRIKDSALIAAATLSHRYITDRFLPDKAID
                     LIDESASRLRMEIDSVPTEIDEIERRVIQVQIEIEAMKKEKDAASLERKRKLEEDLKE
                     MQRDVYERRQHWEKEKALIMGIGEIKEKIDHAKTEADEVERKGDFGRVAEIRYGIISG
                     LEKELQEKNRQLLELQKGRKMLKEEVDEEDIARVVSKWTGIPVSKMLEGDMEKLVKME
                     ERLHNRVISQDEAIEAVANTIRRARAGLQDPNRPLGSFLFLGPTGVGKTELAKALAEF
                     LFDDEQAIVRIDMSEFMEKHSVSRLIGAPPGYVGYEEGGYLTEAVRRRPYSIILMDEV
                     EKAHPEVFNVLLQILDDGRLTDGQGRTVDFKNSVIIMTSNLGSQWITDLGGEDYDEMK
                     RRVMEAVKSHFKPEFLNRIDELIIFKSLSVEDIKKIADLQLAYLVKRVAERDIEISFT
                     DKLKEMVAKEGYDAVYGARPLKRLIQKKIQDPLALLILKGDVQEGDKLKVNIDTKGNV
                     TFK"
     gene            3036..3377
                     /locus_tag="PK125_05950"
     CDS             3036..3377
                     /locus_tag="PK125_05950"
                     /inference="COORDINATES: protein motif:HMM:NF019498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HPB37684.1"
                     /translation="MNTGNLFENIPDRCRAEIFETLVENNHLKIERIISEGHSTPLNE
                     WYDQDWDEWVVLLQGSADILFDGDRTPFSLKPGDYLFIPAHIKHRVEWTDRIKKTIWL
                     AIHMKQTKSEK"
     gene            complement(3396..3770)
                     /locus_tag="PK125_05955"
     CDS             complement(3396..3770)
                     /locus_tag="PK125_05955"
                     /inference="COORDINATES: protein motif:HMM:NF023395.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine triad nucleotide-binding protein"
                     /protein_id="HPB37685.1"
                     /translation="MDEQCRFCQIIRGERNADFVYEDDTLVIFKDAYPAAPVHLLIVP
                     RKHIRSVNDLQEEDQSIIGKMVFKARDIARSQGIADSGYKIHINTERGGGQVIFHLHM
                     HLFGGWKDKPPHIKLMTVNSDQ"
     gene            3896..4084
                     /locus_tag="PK125_05960"
     CDS             3896..4084
                     /locus_tag="PK125_05960"
                     /inference="COORDINATES: protein motif:HMM:NF014636.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HPB37686.1"
                     /translation="MKIELDGHTYEIEKSMEVSRLLKQFSLSRETHLVIVNDRLVTED
                     YRLGRDDRVKLIRVVSGG"
     gene            4081..5034
                     /locus_tag="PK125_05965"
     CDS             4081..5034
                     /locus_tag="PK125_05965"
                     /inference="COORDINATES: protein motif:HMM:NF013348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00269 family protein"
                     /protein_id="HPB37687.1"
                     /translation="MKCRVCGQDAHISLRSYNTALCSQDFISFLEKRVFDTIQKYNLI
                     GNNDTPIVAVSGGKDSLSVWHILNKLGYKADGVYVDLGIGEYSQESLSKIKKTAEGLQ
                     RKFFVFPIRKIFEKGIDEIAKVMRRVPCSACGMIKRYAINKVCMDNGYNVLITGHNLD
                     DEAAALLGNLLYWKEEYLWKKSPFLDGHEGHLSRKVKPLFLCSEREMAAYALISGIDY
                     IHDECPFSINAKSLTYKNILNNLEETSPGTKLQFIKGYLKTIKGIEEKKRDISYCTTC
                     GYPCYGGTCSMCRLLHRFHIKDRIGFEEWGSNLDITQKMMT"
     gene            5042..6412
                     /locus_tag="PK125_05970"
     CDS             5042..6412
                     /locus_tag="PK125_05970"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF013180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HPB37688.1"
                     /translation="MSTIDRLSQLIFNIYEAFTVAFFIRESDHLKCLSSISFAPSFDK
                     SRPVSIENSLPGWVIKHNEPLIIPNFDKDVEALGYYGVDEGIKSFMGYPMEGKGVIIV
                     DSKKKWVFTDKEKKILASFVSLISEEIEKRKKTQDFEDIIEELIIERKIMSLFRNLNL
                     SHISIKEIFVELLSLSGADLCFTGIEKNGKLFIKEILGVNDEDYLMKVCPSGHSIAST
                     VLDGERELLLPYNSGFLKEKALFFSGESIKAKQFFGFPLFSNEVPIGVIGFVSISDRP
                     LHEKSIEPLRNISILLSLYYSSLLMRENLEKVKDFDPVTGSIQFTTFLGFVERSIKKN
                     ERFSLLSVKLGGIDMYNRNMGYEFTNGLLQKVFQIIRHCTGSSSFISRKGGGRFYILL
                     REREAFDVRNILKILNYTIAKNISEDIKIDQNNLTEVGVSHFPDDSQNLWELLDRAKE
                     RRIRSA"
     gene            6428..7459
                     /locus_tag="PK125_05975"
     CDS             6428..7459
                     /locus_tag="PK125_05975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HPB37689.1"
                     /translation="MDFFRKLFGLFSTHFAMDLGTANTLIYMKGEGIILNEPSVVAID
                     NTSGKVIAVGREAKDYIGRTPPNISAIRPLKDGVIADFDVTKAMIKYFLTVVRKDRKI
                     QKPKMVVGVPSGITMVEKKAVVDACFQVGIRDTYLVEEPMAAAIGSEMPIELPRGNMV
                     VDIGGGTTEVAIISLAATAYSESLRVAGDEVDESIVRYLQKKHQIAIGAIAAERLKLD
                     GASVYPAEGRGNAFSVVGKDLLTGVPKNLRITSEEIREAIEEPVSAIIDAIRRALEKL
                     PPEFASDLNESGMVLTGGGALLGGLDHRIEKETGINVYVADDPLLSVVLGAGKALEDM
                     ARYRKVFIN"
     gene            complement(7559..9550)
                     /locus_tag="PK125_05980"
     CDS             complement(7559..9550)
                     /locus_tag="PK125_05980"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF024754.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HPB37690.1"
                     /translation="MKRYVIHKEVRGRIPQIKFEQELNEEQLAVVITDNGPILVIAGA
                     GSGKTRVVTYRVAYLLDRGVSPNGILLLTFTNKAAREMLHRVEHLMKIDTRYIWGGTF
                     HHIGNMVLRQHAEKVGLKKNFTILDNEDAKDLIEVVLKDSKIDRKERRFPKGAILKEI
                     FSYSVNTMKDIETSIQERAPFFYDIKEEIELVSQNYREKKHAINAVDFDDLLFYWHKL
                     LSDYDELRRLYALTFSHILVDEYQDTNKIQGEIVDFMGLINKNVMVVGDDAQSIYSFR
                     GANFQNILDFPSKYPETKVFKLETNYRSTPEILSLANHSIGQNTKQFTKALKSIKKNG
                     TIPVLAPLRDVVQQAEFVAQRVLELRDEGIALDNMAVLYRAHYHCMELQMEFTRRDIP
                     FEIRSGLKFFEQAHIKDVIAFLKVMANPNDEVSWKRALKLFPKIGNRTAEHIYDFLQT
                     LSNPYDAFISKRIADKFKKIHKESIETWSRLFSDLTKLQDQEAPGDMISAVLNNGYIE
                     YLKYNYPNYDTRLEDIGQLVNFSTKYQSVESFLSELSLMSGTSGEEILSANYEDERVI
                     LSTIHQAKGLEWKIVFIIWCAEGRFPNPRALDEGGLEEERRLFYVATTRAMDELYLCY
                     PLLAFDKQVGHIILKPSRFVSELRSTDYEEWQIGDMYQP"
     gene            9783..10175
                     /locus_tag="PK125_05985"
     CDS             9783..10175
                     /locus_tag="PK125_05985"
                     /inference="COORDINATES: protein motif:HMM:NF017876.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HPB37691.1"
                     /translation="MNIILLSIITIASVVLVVVLIYVLLEVRQATRKLERFITMSEDS
                     LKPTLDEFPGTVRSIRHIAENIETVTDDIKTLSSSVRDVGENIRLTSNYIENIAASSS
                     VRVMGLKAGISAGLNALFNNLLAKHKKL"
     gene            10187..10543
                     /locus_tag="PK125_05990"
     CDS             10187..10543
                     /locus_tag="PK125_05990"
                     /inference="COORDINATES: protein motif:HMM:NF024143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HPB37692.1"
                     /translation="MSEEERGYSAGSTLLAFFLGGLVGAGVALLLAPKSGPETRKIIK
                     EYAGEAKGKAEDYLDKFKGSATTVVDKVKESATSVLEKGKEFVEEQKTVLTSAVEAGK
                     DAYQKEKEKLTAEHKA"
     gene            10588..11664
                     /locus_tag="PK125_05995"
     CDS             10588..11664
                     /locus_tag="PK125_05995"
                     /inference="COORDINATES: protein motif:HMM:NF013740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HPB37693.1"
                     /translation="MPANRLYSIALYFFVAVLGYLSYQIFKPFLSPLAWAIVLSIVFY
                     PFFTFVLRFVKWRPLASFITLLIILILLLGPFSYLSYLLSQEIRSIVDEASSGKFDPI
                     GRYLNHPFVSKVINKILAMFHLSQIEFEKKIMENVVLFGKSIVGGITGGIGNIASAIT
                     DFIFMILSIFFFLEGGSEFVEGLKKYIPFSQKQRERLIKQTRGIIVSTIYGGVTVAIA
                     QAIIGGLAFSLLSVPSPVLWGLAMFVASFIPLLGTFIVWGPMAVYLFFKGMILKAIIL
                     VIVGIVAISSVDNILRPLIIRGKVQMPTLAIFFSILGGIKLFGFIGFIMGPLVLALFV
                     SAVQMLQFVEEERNMETADRKGKD"
     gene            complement(11700..12386)
                     /locus_tag="PK125_06000"
     CDS             complement(11700..12386)
                     /locus_tag="PK125_06000"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HPB37694.1"
                     /translation="MSLGHFIEKIPLFAGLVDSQIELLLAIVEKHKHFRGRVIFSEGE
                     EATGFYVLRSGRVKIYKLSLEGKEQILHIFGPGEPFGEVAVFEGRQFPAYAETLEKSE
                     TLFFPRKKFVELIIKDPSMAMNMLAMLSKRLRYFTQLVENLSLKEVPQRLAAYLLVLS
                     KIGDKNDMVELDIAKGQLASLIGTIPETLSRILNKMSGQRIIEVEGRQIKLLDRKSLE
                     SISLGEKLLV"
     gene            12475..13230
                     /locus_tag="PK125_06005"
     CDS             12475..13230
                     /locus_tag="PK125_06005"
                     /inference="COORDINATES: protein motif:HMM:NF013046.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB37695.1"
                     /translation="MKKIPVPYSEDFLTDYRTVDCENPERAWVIQSAIREIADFCEPS
                     LCSRSDLLLCHTESLINSVEIDAEVYSVARRAAGGAIMAAEIALKQPSFALIRPPGHH
                     AGRNFNGGFCFFNNMAIALYVLLSKGLIRNALIIDIDLHYGNGTEDIVKDNPQIMFRN
                     IHAFQREEFLKKVESALSDATSYDIIGCSAGFDTYVHDWGGILLTDDYCEIARMITSA
                     NPHTFTILEGGYYISDLGKNVVSYLKGIQEACL"
     gene            13221..15497
                     /locus_tag="PK125_06010"
     CDS             13221..15497
                     /locus_tag="PK125_06010"
                     /inference="COORDINATES: protein motif:HMM:TIGR00361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0009290 - DNA import into cell involved in
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HPB37696.1"
                     /translation="MFVIAVFFFACGIYLDALYEISLKITIPLTVLLLFFIPFCFKKN
                     SLFSVPLIFICFMFAGMSTLGVVTVTQTIYASGQAKEVYSGLIIESSPNTKILRLTNP
                     MEVSGLKTILRTTQQFNINDNVKVFGELREIHPTFNNPYLLSWKWLKKLEGISYELRG
                     KIISISPGNHIIHGIRNTLKRKIEVSGAQNPGILKALTIGDTTGLDDATKQLFLRTGT
                     SHILAISGSNIGIVTAFFFFIARFLVGRIRKLSLRGDDTRYAALITIPFAILFMLIAG
                     SSIPTIRATIMITIYMLSIFIGRGRSIFNTVALSALVILVIYPHSIFTASFQLTFASV
                     IFIILFSQHILPLFKTGNKVLKWLFLSAGVTLAANFGTFPIVLYHFYGINPLSLIHNL
                     VAVPLMCVVAMPLTLLGTALPWNDCLLRVAGGIITLTTNILKALDWGYIYPIIRPNLF
                     EIALYFFFFFFLLHIRKKIVYVFMAFFVLPLVMGYGYYTYSQLYNNNLRINFIDVGNG
                     DAILIEGPNGMRMLVDGGGFHSSDFDAGKYILTPILLSKKIRTLDYVINTHPHGDHLG
                     GLPTVIRNFTVKNFSTGHYFISQEKFIDLMKLLKEKKILLKIWKGGDKFFLKNGLHIM
                     VLNPDPAAPMEDINNASLVIKLTYNDFTILLPGDIGSNIEEKLVISGNDIRADVLKIP
                     HHGSKHSSCIEFIAAVRPKLAVLSVGKGIPGIPSHETLKRYDAFFIPLLRTDRDGMIQ
                     VWCKNNEVAWSTQKRLIK"
     gene            complement(15530..16459)
                     /locus_tag="PK125_06015"
     CDS             complement(15530..16459)
                     /locus_tag="PK125_06015"
                     /inference="COORDINATES: protein motif:HMM:NF014587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ppx/GppA phosphatase family protein"
                     /protein_id="HPB37697.1"
                     /translation="MNYASIDIGTNAILLMIAEITNTVKEIHDTAVITRLGEGLKQTG
                     ALSFEAMERTINALRGFKTVIDKTRVTGISCVGTAALREARNSAIFIDLVKEQLAFEI
                     RVISEREEAFYTYLSVRQNFLDSKEDIVIIDIGGGSTEIIYGNRENFIDHVSFPVGSV
                     KLTEMFIKHDPPSGDEIRLLIDFIRSTLNFPRKNHVRSVIGTAGTITNLGSISKGLKE
                     WDKKQIHGLILSSKRIDEMAHHLITKTISERSRMAGMEKGREDIILQGVLLLKEIMSY
                     FGAEELIIDTNGVRHGILCERFASLNAQAETVE"
     gene            16584..17237
                     /locus_tag="PK125_06020"
     CDS             16584..17237
                     /locus_tag="PK125_06020"
                     /inference="COORDINATES: protein motif:HMM:NF014713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcdA"
                     /protein_id="HPB37698.1"
                     /translation="MLPLVPSYLIFISGITFDDYREFQSMKYRKIVLIHSLSFIIGFS
                     FVFIILGISSSALGKLLSNYQVYIMRVGGFILIILGLYYLDVVKIPLLNRERVFHLQE
                     KPVGLLGSFIIGITFSLGWTPCVGPALSSILIIASTTESIWEGIFLLGLYSLGLAIPF
                     LISALIFDQLFGLLKKYSAVVRYSMKFLGVLFVIIGILFFSSYWNLVIAKISQIFAL"
     gene            complement(17234..18136)
                     /locus_tag="PK125_06025"
     CDS             complement(17234..18136)
                     /locus_tag="PK125_06025"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: protein motif:HMM:NF005143.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-32"
                     /protein_id="HPB37699.1"
                     /translation="MTKDIFSENQKELIKPLEDKGLPIPYDPLKKYLSEVSKYPVLTR
                     EEELEISTKVFKEKDRSAAQKLAISNLKLVVKIALEYYNTYLNILDLIQEGNVGLLHA
                     VKKYNPYKGTKFSTYASFWIRAYILKYIMDSWSLVKVGTTQGQRKLFYRLNKEKQRLE
                     AQGIYPAPKLLASTLDVKEEEVEEMQKRLTYTDISLEAPVHDESDDTIMDMMRSGENV
                     EEVVAEKEKSDILAMKVAEFKKTLNDKEIFIFEQRVMAEEPLTLQEIGARFSISRERV
                     RQIENRVLKKFKERFKGEIEDLDF"
     gene            complement(18133..19137)
                     /locus_tag="PK125_06030"
     CDS             complement(18133..19137)
                     /locus_tag="PK125_06030"
                     /inference="COORDINATES: protein motif:HMM:NF024869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC family protein"
                     /protein_id="HPB37700.1"
                     /translation="MSIYDCVVKYLNEGKKGILATVVSKEGAAPREEGAKMLVGEDEK
                     TYGTIGGGELEAKVIREALELMESNNVKLFHARMDAKAVEDEGMLCGGNVDVLLEPIL
                     ERHKIVYENIQACEKKGRRAVVVTRFRKAILQKSLIDHDGRIWGDSLGKEEVTSLRDF
                     WNEHRVRFVNGNTVIEPLSIFSNLYIFGAGHVSQFLSQVAKLVEFNVTVIDDRKDFAN
                     KDRFPEADTIIVEDFNKVFDTLDLTTNSYIVIVTRGHSHDALVLEKCIEKQTSYIGMI
                     GSKRKVRMVLEYLKEKGVRKEILESIYAPIGIDINSETPQEIAISIVAQLIKVRGLRN
                     "
     gene            complement(19148..>19433)
                     /locus_tag="PK125_06035"
     CDS             complement(19148..>19433)
                     /locus_tag="PK125_06035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="molybdenum hydroxylase"
                     /protein_id="HPB37701.1"
                     /translation="LRSPRAGLVKHVKELGETVKKGELILYVDDVPVRASIDGLLRGL
                     IREINVIENEKVGDIDPRGVTEYCFTITEKARAIGGGVLEAILHEFNRRA"
BASE COUNT         5279 a         4289 c         3868 g         5997 t
ORIGIN      
        1 tttgatttta atggtaggcg cggtcggtat tgaaccggcg acctctaccg cgtcaaggta
       61 gcgctctccc actgagctac gcgcctattg tagatgcaat ttatagcagt aaccatattg
      121 attgtcaagc gaaattgatt tatcacaggg tttctgacca cgaagcatca agttaaacgg
      181 gtgaccggaa attgcccttc cagtcacccg tttaaccgtt acttaaaagt tacatttcct
      241 ttggtatcaa tgttgacctt gagtttgtcc ccttcctgaa catcgccctt gagtatcaac
      301 aatgccagag gatcttgtat ctttttctgg ataagccgtt ttagcggccg tgcgccatac
      361 actgcatcat agccttcttt agccaccatt tctttcaact tgtcggtaaa tgagatctct
      421 atatctctct ctgcaactct cttgaccaga taggcgagtt gcaaatctgc tattttcttg
      481 atatcttcaa cagagaggct cttgaaaatg atcagctcat cgattcggtt caggaattca
      541 ggcttgaaat ggcttttcac agcctccatg accctgcgct tcatctcatc ataatcctct
      601 cctccgagat ctgttatcca ttgacttccc aggttggagg tcataattat aacagagttc
      661 ttaaaatcaa ctgtcctgcc ctgcccatcc gttagtcgac cgtcgtcaag tatctggaga
      721 agtacattga acacttcagg atgggccttt tctacttcgt ccataaggat gatcgagtaa
      781 ggtcttcttc taactgcttc agttaagtac ccgccctctt catatccaac atagccagga
      841 ggagcaccta tgagtcggga tacagaatgt ttttccatga actcactcat gtctatccga
      901 acgatcgcct gttcgtcatc aaagagaaat tcagccaatg ccttcgccag ttctgtcttg
      961 cctacgccgg ttgggcccag gaagagaaac gaaccaaggg gcctattggg gtcctggagt
     1021 cctgccctgg cccgtcgaat ggtatttgca acagcctcaa tagcttcatc ctgacttatc
     1081 acacgattgt gaagtctttc ctccatcttg accagctttt ccatatcacc ttcaagcatc
     1141 tttgaaacag gaatcccagt ccacttcgac actactctcg ctatatcttc ctcgtctact
     1201 tcctctttca gcatcttccg acccttttgg agctccaaga gctgcctgtt tttctcctgt
     1261 aattcttttt caaggccgga aataatgcca tatcgaattt ctgccacgcg accgaaatca
     1321 ccttttcgct caacctcgtc tgcctctgtt ttcgcatgat caatcttttc ttttatctcg
     1381 ccaatgccca tgatgagcgc tttttccttt tcccagtgtt ggcgcctttc ataaacatcg
     1441 cgctgcattt ctttcaaatc ttcctctaat ttcctctttc gttcgaggga cgctgcatct
     1501 ttctcctttt tcattgcctc aatttcgatc tgtacctgga taactcttcg ctcaatctca
     1561 tcaatctctg ttggaacact gtctatttcc atcctgaggc gcgaggcaga ttcatctata
     1621 agatcgatgg ccttgtcagg caggaatcta tctgtaatat agcgatgtga cagggtggca
     1681 gctgcaataa gggcagagtc tttgattctt acgccgtggt gaagctcata tttctcttta
     1741 agacctctta gtatagcaat cgtctcctca actgaaggct caccgacata gacaggttgg
     1801 aaccttcttt ccagtgctgc atccttttct acgtattttc tatattcatc cagggttgtt
     1861 gctcctatac aacggagctc acctcgtgca agggcaggct tgagcatatt cgatgcatcg
     1921 attgcaccct gggcactgcc tgcacctacg atggtgtgaa gctcatcgat gaagagtatt
     1981 atagctcctg cagcctcgtc tatttctttg aggactgctt tgagcctgtc ttcgaattct
     2041 cctcgatatt ttgcccctgc aagcattgct ccgaggtcaa gggaaagaac ctttttgttt
     2101 ttcaacgtct caggaatatc accactcaca attcgctgag caagcccttc tacaatagcc
     2161 gtcttaccaa cacctggctc accaatgaca acaggattat ttttggtcct cctcgacagg
     2221 acctgcataa cccttctcac ctcttcgtcc cttccgataa caggatcgag tttgcccttt
     2281 cgggcctctt ctgtcaaatc cctgcagtac ttctgaagtg cctggtattt ctcttcaggc
     2341 gcctggtctg taaccctctg tgcgccccgg atatctttga gtactgagta gatccggtcc
     2401 ttagtaaccc cttgtccttg aagtattctc cctgctgctc cattacctgt atctgtgaca
     2461 ccaataagca gatgctccgt acttatgtat tcatccttca atctctctgc ctcgataaaa
     2521 gccgtatcga gagtctgctt catccttggg gttatataaa cctgtgctgc cccatggaca
     2581 gcaggtatat ttttaagttc tttttcgagg tcttgcaata ttgctccaga attggcacca
     2641 agtttgtcta gtataggtct gacaatgcca tccttttgtg tcaccaaggc ataaagaaga
     2701 tgttcgttgt caatcccctg atgatgggac gcctctgctt ttttctgtgc ctctgagatt
     2761 acctcttgag ctttaattgt caacttttcc cagttcattc tatcacctca tatctttgaa
     2821 gatcagtaca tgtcgaccag ctttcaactt aaatccaaat ttaattcact tgtcaggtga
     2881 tgttttagct gttttagaac agcaaatcaa aactctttca aattgctgat cgcttgttat
     2941 attggaaaat ataagcattg aattgccgaa tgcaatgctg tccatccttt cgacattgta
     3001 ttagatccag gcatgtgata aggtaaattg agcttatgaa cacagggaac cttttcgaaa
     3061 atatacctga caggtgcagg gcagaaattt ttgaaaccct tgtcgagaac aaccacctca
     3121 agatcgagcg aatcatatct gaaggccact caaccccttt aaacgaatgg tatgatcagg
     3181 actgggacga gtgggtagtc ttgcttcagg gtagtgctga tattctattc gatggcgata
     3241 ggaccccttt ttcgctaaaa cccggggatt acctctttat ccccgctcac ataaaacatc
     3301 gggttgaatg gactgataga attaaaaaga caatctggct ggcaatccat atgaaacaaa
     3361 cgaaaagtga gaagtaaaaa aatggggaaa acagttcatt gatcactgtt tactgtcatt
     3421 agctttatat ggggtggttt atctttccag ccaccgaaga gatgcatatg aaggtgaaag
     3481 ataacctgac cccctccccg ttcggtgttg atgtggattt tgtagcctga atcagcaata
     3541 ccttgactgc gagcaatatc tcttgcttta aaaaccattt tccctatgat tgactgatct
     3601 tcctcttgca gatcattgac actccttata tgtttccgag gaacaatcag aagatggacc
     3661 ggcgcagcag ggtatgcatc tttaaatata acaagcgtat catcctcgta gacaaaatca
     3721 gcattcctct cgcccctgat aatctggcaa aaccgacatt gctcatccat ttgtaagcac
     3781 ccggtatatg ttaacaggat attgctccga gaagcaatgg aaactcgctt ttcttgtcct
     3841 tcacaccata ttctctttcc tttacttcat cgcagtcttg tggtaagtaa aagccatgaa
     3901 aatagaatta gatggacata cgtacgaaat tgagaaatct atggaggtct cacgcctgct
     3961 caagcagttt tcgctaagtc gggaaactca ccttgttatc gtaaatgacc gtctggttac
     4021 cgaagattac cggctcggaa gagatgaccg ggtaaagctc atcagggtcg tatctggcgg
     4081 atgaagtgca gggtttgcgg acaagatgcg cacattagtc ttcggtcgta taatacagcc
     4141 ctgtgctctc aagattttat ctccttttta gaaaaacggg tttttgatac cattcaaaaa
     4201 tacaatctga ttggaaataa tgatacaccg attgttgctg tctcaggagg aaaagatagc
     4261 ctctctgttt ggcatatcct caacaagctc ggctataagg cggacggagt ttacgtagat
     4321 ctcggcatcg gcgaatattc ccaggaatct ttatcaaaga tcaaaaagac agctgaaggc
     4381 ttacagcgaa agttttttgt gttccctatt cgaaaaatat ttgaaaaagg catcgacgaa
     4441 attgctaaag tgatgcgccg tgtaccatgc tctgcttgtg gtatgataaa acgatatgca
     4501 ataaacaagg tttgcatgga caatggatat aatgtactta ttacgggtca caatctcgat
     4561 gatgaggctg cagcactcct tggtaatctg ctttactgga aagaagagta cctgtggaaa
     4621 aaaagcccat tccttgacgg tcatgaggga cacctatcca gaaaggtaaa acccctcttc
     4681 ctctgttctg aacgagagat ggcggcatat gcattgattt cagggataga ttatattcat
     4741 gatgaatgtc cattttccat taacgctaaa tcactcacat ataaaaacat attaaacaat
     4801 cttgaagaaa cttctccggg gactaaactc cagttcatca agggctactt aaaaacaatc
     4861 aagggcattg aagagaagaa aagagatatt tcatattgta ctacatgtgg atatccctgt
     4921 tatggaggga catgctccat gtgcaggctt ctacatagat tccatattaa agacagaata
     4981 ggttttgagg aatggggcag caaccttgac ataactcaaa aaatgatgac ataattccgt
     5041 gatgagtaca attgatagac tttctcaact tattttcaat atctacgaag catttacagt
     5101 agcttttttt atcagggaat ctgaccattt aaaatgtctt tcgtcaatct cattcgcacc
     5161 cagctttgat aagtccaggc cagtatcaat tgaaaacagc cttcctggat gggtaataaa
     5221 gcataatgaa ccgcttatta tcccaaactt tgacaaagat gttgaggcgc tcggttatta
     5281 tggtgtagat gagggcatta aatctttcat gggatacccc atggagggaa agggtgtaat
     5341 catcgttgat tctaagaaga aatgggtatt tacggacaag gaaaagaaaa tcttagccag
     5401 cttcgtctct cttattagtg aagagattga aaagagaaag aagacccaag atttcgaaga
     5461 cattattgag gaactgatta tagaaagaaa aattatgagc ctttttcgca atcttaatct
     5521 ttctcacatt tccatcaaag agatattcgt cgaactattg agcctttcag gtgctgatct
     5581 ttgctttact ggtattgaaa aaaatgggaa gttattcatc aaagaaattt taggggtaaa
     5641 tgacgaggac tacttgatga aggtatgccc ctctggacac agcatagcat caactgtttt
     5701 ggatggagaa agagaactcc tactccctta caacagcggc ttccttaaag aaaaagccct
     5761 ttttttttcc ggtgaatcga taaaagcgaa acaattcttc ggatttcccc ttttttctaa
     5821 cgaagttcct ataggggtga taggttttgt gtctatctca gacagacctc ttcatgaaaa
     5881 atctatagaa cccctcagaa atatatcgat cctcctttcc ctttattact cctcgttgtt
     5941 gatgcgggaa aatcttgaaa aggtaaaaga ctttgaccct gttaccggat ctattcaatt
     6001 taccactttt cttggctttg tggagagatc tataaaaaag aatgaacggt tttctctcct
     6061 ttcagtaaaa cttggtggta tagatatgta taaccgtaac atgggatacg aattcacgaa
     6121 tggtctcctt cagaaggttt ttcaaatcat ccgtcattgt actggtagca gttcgttcat
     6181 atctcggaaa ggggggggcc gcttttacat cctacttaga gaaagagagg cctttgacgt
     6241 aagaaacata ttaaaaatcc tgaattacac tattgcaaag aacatttccg aagacatcaa
     6301 gatagaccag aataacctga cggaagtggg ggtatctcat tttcctgatg atagtcaaaa
     6361 cctctgggag cttctggatc gcgcaaagga aagaaggata agatctgctt aatgacaagg
     6421 aggggttatg gatttcttta gaaagctttt tggcttgttt tccacacatt ttgccatgga
     6481 ccttggtact gccaatacac ttatttacat gaaaggtgaa ggtattattc taaatgaacc
     6541 ttcagtagtg gctattgata atacatctgg taaggtcatt gccgttggca gagaagcaaa
     6601 ggactacatc ggacgaacac ctccgaatat cagcgccata cgcccgttaa aggatggcgt
     6661 tattgcggac tttgatgtta caaaggcgat gataaaatat tttcttaccg ttgtgaggaa
     6721 agatagaaaa atacagaaac ccaaaatggt tgtgggtgtg ccttcaggca ttaccatggt
     6781 cgaaaaaaaa gcagttgttg atgcctgttt tcaggtgggt atcagggaca cctatcttgt
     6841 tgaagaacct atggctgcag ccataggttc ggaaatgccc attgaattgc ctagaggcaa
     6901 catggtggtt gacatcggcg gaggaacaac tgaggtggca attataagcc ttgctgcaac
     6961 tgcctacagt gagtccttga gggtggcagg tgatgaagtt gacgaatcaa ttgtaaggta
     7021 tctccagaag aaacatcaga ttgccatagg agccattgcc gcagaaagac tcaaacttga
     7081 cggggcatca gtctaccctg cagaaggccg aggtaatgct tttagtgtag ttggcaaaga
     7141 tcttctcacc ggtgttccga aaaatctgag gatcacaagt gaagaaatac gggaggctat
     7201 agaagaacct gtttcagcaa ttatcgatgc catacgcaga gcccttgaaa aactacctcc
     7261 tgaatttgca tctgacttga atgaatcagg tatggtttta actggtggcg gtgctttact
     7321 gggtggtctt gaccacagaa ttgaaaaaga aacagggata aacgtttatg ttgctgatga
     7381 tcccttgctg tccgttgtcc tcggtgcagg aaaggctcta gaagacatgg caagataccg
     7441 taaggttttt attaattaga actccttaag catattcagc tttcaggaaa aacaatcgaa
     7501 aatcaggcga ccgggggtac taacagattt attgatctgt acaccaatgg gtggcagctc
     7561 agggctgata catatccccg atctgccatt cttcataatc agttgatcga agctccgaga
     7621 caaaacggga gggtttcagg atgatatgcc ccacctgttt atcaaacgcc aggagcggat
     7681 aacacaagta tagctcatcc atagcccgtg tggttgcaac gtaaaacagc cttctctcct
     7741 cctccagacc tccctcgtcc aaagcccttg gatttggaaa cctcccttct gcacaccata
     7801 tgatgaatac aatcttccac tcaagccctt ttgcctgatg tatcgtactg agaattactc
     7861 tttcatcttc atagtttgcg gataaaatct cttcaccgct tgtgccactc atgagcgaaa
     7921 gctcgcttaa aaagctttcc acggactgat acttcgtgga aaaattcacg agttgtccaa
     7981 tatcttcaag ccttgtatca tagttagggt agttatactt gagatattct atgtaaccat
     8041 tatttaatac ggcagaaatc atgtcgccag gtgcttcctg atcctgtaat tttgtaagat
     8101 cactgaataa cctagaccaa gtttctatac tttccttgtg aattttttta aatttgtcag
     8161 caattctctt agaaataaaa gcatcatacg ggttactcag ggtttggaga aaatcataga
     8221 tatgctcggc cgttctattg cctattttgg gaaatagctt gagggccctt ttccatgata
     8281 cctcatcgtt tggatttgcc attaccttta agaatgcaat gacatctttg atgtgagcct
     8341 gttcaaaaaa tttgagccca cttctgattt caaaaggtat gtcacgcctg gtaaattcca
     8401 tctggagttc catacagtga tagtgtgctc tgtaaagaac tgccatgttg tcaagagcta
     8461 tcccttcgtc tctcagttca agaactcttt gtgcaacaaa ttctgcttgc tgaaccacgt
     8521 cgcgtaaagg tgcaagaacc ggaatagtac catttttttt aatgctttta agggctttgg
     8581 taaattgttt tgtgttctgg cctattgaat ggttggcaag gcttagaatt tcgggcgtac
     8641 tgcggtaatt ggtttcaagc ttgaatacct ttgtttcagg atacttactg gggaaatcca
     8701 gtatgttttg aaaatttgct cctctgaacg aatatatgct ctgcgcatca tcacccacca
     8761 ccatcacgtt tttattgata agccccatga aatcaactat ctcaccttga attttgttcg
     8821 tgtcctggta ctcatccacc aggatatgag agaatgtcag ggcgtagagt ctcctcaatt
     8881 catcataatc agaaagaagt ttgtgccagt aaaaaagcag gtcgtcaaaa tcgactgcgt
     8941 tgattgcgtg ttttttttcc ctataattct gtgatacgag ttctatttcc tctttaatat
     9001 cataaaagaa cggtgccctc tcttgtatgc tcgtttcaat atctttcatg gtattcactg
     9061 agtaactgaa tatttctttg aggattgcgc ctttaggaaa acgcctttct ttccggtcga
     9121 tctttgaatc tttaagaaca acttcgataa ggtctttggc atcctcatta tccagtattg
     9181 tgaaattttt tttaagacca accttctccg catgctggcg taataccatg ttacctatat
     9241 gatgaaaagt accaccccaa atataacgtg tatcgatctt cattaggtgt tcaaccctgt
     9301 ggagcatttc tcgtgcagcc ttattggtaa aagtaagaag gaggatgccg tttggagaga
     9361 cacctctatc caggagatat gcaacccggt atgtcacaac tcttgtcttt ccgctgcctg
     9421 cccctgctat aacaagtatc ggtccattat cggttatcac gacagcaagt tgttcctcgt
     9481 tgagttcctg ttcgaactta atttgaggta ttctacctct tacttcctta tggatgacat
     9541 atcgtttcat gacggtaaag tatgcttcat ggggtcgcac ttgtcaagag accaagaaca
     9601 atgcaggatt aagaggacag aggttcgtgg gttcaaaaaa atcttgaagc aagccaaaaa
     9661 aatacaccct gatagtcctt gacagtcctt ggatgctctg ctacgattgt ttcgtagaat
     9721 attttcttaa acctatggca gaggggggtt tgcaatgttt atattatggg aggtcaatga
     9781 taatgaatat cattttgctc agcattatta caatcgcatc cgttgtactc gttgttgtac
     9841 tgatttatgt tcttttggag gtacggcaag caacacgtaa gcttgaacgt tttataacca
     9901 tgtcagaaga ctcattaaaa ccaacccttg atgaatttcc tgggacagtg agaagcatcc
     9961 ggcatattgc agaaaacatc gaaactgtta cagatgacat aaaaactctt tcgagctcag
    10021 tgagagacgt tggtgaaaat ataaggttaa cgagtaatta cattgaaaat attgcagcat
    10081 cgtcttcagt tcgggttatg ggcctaaaag cagggatcag tgcaggcctt aatgccttat
    10141 tcaataattt gttggcaaaa cataaaaaac tataaggagg tcaactatgt cggaagaaga
    10201 aaggggatat agtgcaggtt ctacccttct cgcatttttt cttggaggat tagtcggggc
    10261 aggcgtagcg ctgcttctcg caccaaaatc aggaccagaa acgaggaaga taatcaaaga
    10321 gtatgccgga gaggcaaagg ggaaagcaga agattatctg gacaagttta aaggcagtgc
    10381 aactactgtg gtagacaagg ttaaagaaag cgctacatca gttctggaaa aagggaaaga
    10441 gtttgtagaa gaacaaaaaa cagtgttaac atcagctgta gaagcaggca aagatgcata
    10501 tcaaaaagaa aaggaaaaat taacggctga acacaaagcc taacgataat caggaacaca
    10561 ctttagggtt ctttatactc aaggtctatg cctgctaatc gtttatattc aatcgcgctc
    10621 tatttttttg tagctgttct cggttattta agttatcaga ttttcaagcc ttttctttct
    10681 cctttagctt gggctatcgt gctgtccatt gtcttttatc ctttcttcac ttttgtcctg
    10741 aggtttgtga aatggaggcc ccttgcttct tttattaccc tattaatcat actaatactt
    10801 ctcctagggc cattttcata tctttcctat ctactttctc aggaaatacg atcaatagtt
    10861 gatgaagctt ccagtggcaa atttgatcca atcggcagat atctgaacca tccatttgta
    10921 agcaaggtaa ttaataaaat ccttgccatg tttcatttat ctcaaatcga atttgaaaaa
    10981 aagataatgg agaatgttgt gctttttgga aaaagtattg tagggggcat cacaggtggg
    11041 attggtaaca ttgcctcggc cataacagac tttattttta tgattctttc catattcttc
    11101 tttctcgagg gaggttcaga atttgtcgaa ggactaaaaa aatacatacc cttttcccaa
    11161 aaacaaagag aacgattgat taaacaaact cgggggatta tcgtctcaac aatatacggt
    11221 ggagtaaccg tagcaatagc tcaagctatc attggtgggc ttgctttctc acttctgagc
    11281 gtcccttctc cggtgctctg gggtcttgcc atgtttgtag cttcctttat acctcttctg
    11341 ggaacgttca tagtctgggg acctatggca gtgtatctgt tttttaaagg gatgattctt
    11401 aaggccataa tacttgtcat tgtagggatt gtcgcaataa gttcagtaga caatatcctg
    11461 aggccattaa ttatcagagg caaggtgcaa atgcccaccc tcgccatctt tttcagcata
    11521 ctcgggggta taaagctctt cggatttata ggatttatca tgggacccct tgtattagct
    11581 cttttcgtat ctgcagtcca gatgcttcaa tttgttgaag aagaaaggaa catggaaacg
    11641 gcagaccgga aaggcaaaga ttaagaaaaa acaggattgg ataatccgtc gaatcatttt
    11701 tataccaaga gtttttctcc tagcgagata ctttcaaggg attttctgtc gagaagcttt
    11761 atttgtctac cttcaacttc tataatacgt tgacctgaca ttttattgag aatccgcgat
    11821 aatgtttcag ggatcgtccc aattaagcta gcaagttgac cttttgcaat atcaagttct
    11881 accatatcgt ttttgtctcc aattttactg aggacaagta gatatgcagc aagcctttga
    11941 ggcacctcct tgagggaaag attttccacg agctgcgtga aatacctgag gcgctttgaa
    12001 agcatggcaa gcatattcat agccattgaa gggtctttaa tgattaactc aacaaacttc
    12061 ttgcgtggaa aaaatagtgt ttcgctcttc tccaatgttt ctgcataagc tggaaactgt
    12121 ctgccttcga acacagccac ctctccaaaa ggttctccgg gcccaaagat atgtaggatc
    12181 tgctccttac cctccaaaga gagtttgtat atcttcactc gtcctgagcg aagaacgtaa
    12241 aatccggtag cttcctcacc ttctgaaaag attaccctgc cacggaaatg tttatgtttc
    12301 tcaactatgg caaggagtaa ttcaatctgt gaatcgacaa gccctgcaaa gagaggaatt
    12361 ttctctatga aatgtccaag actcatgctt ttcatctatc atggttcaag caatacgtca
    12421 aattgttcat gaggaatgtt tgacattggc agagctttgt ggtttaaagg agtgatgaaa
    12481 aagattccgg tgccttatag tgaagatttc ttaactgatt ataggacagt agattgcgaa
    12541 aatccggagc gagcctgggt aattcagtct gcaattcgag agatcgccga tttttgtgaa
    12601 cccagtttgt gtagccgatc agaccttctc ctctgccata cagaatcgct tattaattcg
    12661 gttgagattg atgcagaggt gtattcggtg gctcgtaggg cagcgggggg agccatcatg
    12721 gctgctgaaa tcgcccttaa gcaaccttct tttgctctga tcagacctcc tggtcaccac
    12781 gctggtcgaa atttcaatgg cggcttctgc ttcttcaata acatggctat agccctctat
    12841 gttctcctct caaaaggctt aatccgtaat gcacttatca ttgatatcga tcttcactac
    12901 ggtaatggaa ctgaagatat tgtcaaagac aatccgcaga tcatgttccg taatatccat
    12961 gcgtttcaga gagaagagtt tctcaaaaag gttgagtcag ccctcagcga tgccacctct
    13021 tatgacatta ttggctgttc tgcaggcttt gacacctacg ttcatgattg gggtggtatc
    13081 cttttgaccg atgactattg tgaaattgca cggatgataa cctcggcaaa cccccacaca
    13141 tttaccatac tcgaaggggg ttattacatc tccgatcttg gtaaaaacgt tgtttcgtat
    13201 cttaaaggca tccaggaagc ttgtttgtaa ttgctgtttt ctttttcgcg tgcggaatct
    13261 atctcgatgc cctttacgaa atctctttaa aaattacaat acctctgaca gttctcttac
    13321 tgttcttcat acctttttgt ttcaaaaaaa atagtctctt ctctgttcct cttatattca
    13381 tctgttttat gtttgcaggc atgtcaacgc ttggggtggt aacagttact caaaccatat
    13441 atgcaagtgg ccaggctaag gaggtgtatt ctggtctcat tattgaatca tcacccaaca
    13501 caaaaatatt aaggcttacg aaccccatgg aagtctcagg tttgaaaacg atacttagaa
    13561 caacacaaca gttcaatata aatgacaacg tcaaagtatt cggtgaactc agagagattc
    13621 acccaacttt caacaacccc tatctgttat catggaaatg gctaaaaaaa cttgagggca
    13681 tatcatatga actcaggggt aaaattattt ccatttctcc gggcaaccat ataattcatg
    13741 gaataagaaa caccctcaaa cgcaaaattg aagtttcagg cgcacagaat ccaggcatat
    13801 taaaagccct tactataggt gataccaccg gcttagatga tgcaacaaaa caattgtttc
    13861 tccgcacagg aacttctcat atcctcgcaa tttcggggtc gaatattggg atagtaacag
    13921 cttttttctt cttcatagct cggttcctcg tagggcgtat tcggaaactg agtttaaggg
    13981 gagatgacac acgttatgcc gcccttatca caatcccatt cgctattctg tttatgctta
    14041 ttgccggttc aagcatacca accataaggg caaccattat gatcaccatt tatatgctat
    14101 ctatattcat cggacgagga agaagtattt tcaacaccgt tgcccttagt gcgctcgtca
    14161 tacttgtcat ctacccccat tcgattttta cagcatcttt tcagctcacc tttgcaagtg
    14221 tcatatttat tatcctattt tcccagcaca tacttccgct tttcaagacg ggaaataagg
    14281 tgctgaaatg gctatttctt tccgcaggcg taactcttgc tgcgaacttt ggaacttttc
    14341 ctattgtctt gtatcatttt tatggaataa accccctgtc gttaatccat aaccttgttg
    14401 ctgttccgtt gatgtgcgtt gttgctatgc cattgaccct cctcggcacc gctctgccat
    14461 ggaatgattg cctgctccgt gttgctggag ggattataac actaacaaca aatattctta
    14521 aagctctcga ctggggctat atttatccca taattcgtcc aaatcttttt gagatagccc
    14581 tttacttttt tttcttcttt ttcttattac acatcagaaa aaaaatagta tatgtcttca
    14641 tggctttttt tgttctccca ttggtaatgg ggtacggata ttacacctat tcacagctat
    14701 acaataataa cttacgtatc aattttatcg atgtggggaa cggtgatgca attctcattg
    14761 agggaccaaa tggtatgaga atgcttgtcg atggcggagg atttcattca agtgacttcg
    14821 atgcggggaa atatatatta acccctattc tgctctcaaa aaaaatacga acccttgatt
    14881 atgtcatcaa cacccaccct catggtgatc acttgggtgg cttacccact gttattcgta
    14941 attttacagt caaaaacttc tccacgggac actatttcat ctcccaggaa aaatttattg
    15001 atctcatgaa gctactgaag gagaagaaga ttctgttaaa aatatggaaa gggggagata
    15061 aattttttct gaaaaacggt cttcacatta tggttctaaa cccagaccca gcggccccaa
    15121 tggaggacat taataatgca tctcttgtaa taaagttaac gtataatgac tttaccattc
    15181 ttctgcccgg tgatatcggc tcaaacatag aagaaaaact tgtcattagt ggaaatgata
    15241 ttagggcaga tgtactcaaa attcctcacc atggcagcaa acactcgagt tgcatagaat
    15301 tcatcgctgc tgttcggcca aaattggctg ttttaagtgt tggaaaagga atacctggta
    15361 tacccagcca tgaaacgctg aaaaggtatg atgcattctt cattccctta cttcgcactg
    15421 acagggacgg tatgatccag gtttggtgta aaaacaacga ggttgcctgg tcaacacaaa
    15481 agagattgat caagtaaaaa aaaagaattt gaacactcaa attgatgacc tattcgacag
    15541 tttctgcctg ggcattgaga gacgcaaacc tctcacacag gataccgtga cgtaccccat
    15601 tggtgtctat aattaattct tctgcgccga aatagctcat aatctcctta agaagtaaga
    15661 ccccctgaag gataatgtcc tcacgtccct tttccatacc tgccattcta cttcgctctg
    15721 atattgtttt tgtaattaga tggtgggcca tttcgtcaat gcgtttacta ctcagtatca
    15781 atccatgtat ctgttttttg tcccattctt taaggccctt gctgatactg ccaaggttgg
    15841 ttatggtgcc tgctgttcca atgactgatc ttacatgatt tttgcgggga aagtttaggg
    15901 tggacctgat aaaatctatt aacaatcgaa tttcatcacc tgaaggtgga tcgtgcttta
    15961 tgaacatctc ggtaagctta acgcttccaa caggaaaaga gacatgatcg ataaaattct
    16021 ctctattgcc gtagatgatt tctgtgcttc caccaccgat atcaataata acaatgtctt
    16081 cttttgagtc taaaaaattt tgacgcactg agaggtaggt ataaaaagcc tcttcacgct
    16141 cgctgattac acgaatttca aaggcaagtt gctccttaac aagatcaata aatatggcac
    16201 tgtttcgcgc ctcccgtaaa gctgccgtcc ctacacagga gatacccgta accctggttt
    16261 tatcaattac tgtcttgaag cctctcaacg catttatcgt tctttccatt gcttcaaatg
    16321 aaagagcacc ggtctgtttt agcccctctc ccagacgtgt gataacggca gtatcatgga
    16381 tttccttgac cgtgttcgtt atttccgcaa tcatgagaag gattgcattg gtccctatgt
    16441 caatggaagc ataattcatt tgacaaccca atgaaaatca cactatgata gcacccatgg
    16501 tttcaaccgg catgctctcg tcgacagatt tttctggtat cgttgctttt atttcaggga
    16561 ttctctcctt tttcagtccc tgcattcttc cactcgtccc ctcctattta attttcataa
    16621 gtgggattac ttttgatgat tatagagaat ttcaatcaat gaagtacagg aaaattgtcc
    16681 ttatccattc actgtcgttc attattggtt tctcattcgt cttcataata ctcggaattt
    16741 catcatccgc actaggcaag ctattatcga attatcaggt ctatatcatg agggttggtg
    16801 gttttattct cattatcttg ggcctttact accttgatgt ggtaaaaata ccacttctca
    16861 acagagaaag ggtattccac cttcaggaga aacctgttgg tcttctcggc tccttcatca
    16921 ttggcataac attctccctc ggctggacac catgtgtagg acctgctctt tcatcaatcc
    16981 tcatcattgc ctcaacaact gaaagcatat gggaggggat tttcctgctt ggtctctatt
    17041 ctcttggtct tgcaattcct tttttaatct cagccctcat ttttgatcag cttttcggtt
    17101 tgctgaaaaa atacagtgct gttgtcaggt attcaatgaa gtttctcggt gttcttttcg
    17161 taattattgg gatacttttc tttagctcct actggaatct ggtgattgca aaaattagcc
    17221 agatatttgc actttagaaa tctaaatctt ctatctctcc cttaaaacgc tctttaaatt
    17281 ttttcaagac acgattctca atctggcgca ccctctcacg ggaaatgcta aaacgagctc
    17341 ctatttcctg taaggtcaga ggctcctcag ccataacacg ctgttcaaaa atgaaaattt
    17401 ctttgtcatt taaggttttt ttgaactctg ccaccttcat ggcaagaata tcactctttt
    17461 ctttttctgc aaccacctct tcaacattct cacctgacct catcatatcc ataattgtat
    17521 catcgctctc atcgtgaaca ggggcttcaa gagaaatgtc agtataggta agcctctttt
    17581 gcatttcttc cacttcttct tctttcacgt caagagtgct tgcgagcagt tttggcgcag
    17641 gatatatccc ctgggcttcc agtctctgct tctccttgtt cagccgatag aagagtttcc
    17701 tttgcccctg cgttgtacct accttaacaa ggctccacga gtccatgatg tacttcagga
    17761 tataagcccg tatccagaat gaggcatacg ttgaaaattt ggttcccttg tatggattgt
    17821 attttttcac agcatggaga agtcctacat taccttcctg aatcaagtct agaatgttca
    17881 ggtacgtgtt gtagtattca agagcaatct ttacaacgag tttcaggttt gaaattgcaa
    17941 gtttctgtgc agcagacctg tccttttctt tgaacacctt tgtagatatc tcaagttctt
    18001 cttccctcgt gagtactgga tatttcgaca cctctgaaag atactttttc agtgggtcat
    18061 agggtatggg aaggccttta tcttcaaggg gtttgataag ttctttttgg ttttcggaaa
    18121 aaatgtcttt tgtcaattcc tcaatcctct cacctttatg agctgtgcta cgatactaat
    18181 agcgatctcc tgaggcgttt cagagttgat gtcaatgcca atcggcgcgt aaatagattc
    18241 caggatttct tttcgaaccc ctttttcttt caaatattcg aggaccatgc gaacctttct
    18301 tttgctgcca atcatgccaa tataagatgt ctgtttctca atgcattttt caaggacaag
    18361 cgcatcatga ctgtgtccgc gggttacgat tactatgtat gaatttgttg tcagatcaag
    18421 ggtatcaaaa accttgttaa agtcttctac aattatggta tcagcttcgg gaaacctatc
    18481 tttgtttgca aagtctttcc tgtcatcgat aaccgttaca ttgaattcta ccaatttcgc
    18541 aacctgagat aaaaattgtg aaacatggcc agcgccaaaa atatacaggt tggaaaagat
    18601 cgataacggc tcgatcactg tattgccatt cacgaaacgg actctgtgtt cattccaaaa
    18661 atctctgagg gatgtaactt cttcttttcc taaactatca ccccatatac gtccgtcatg
    18721 atcaatgaga cttttctgga ggatcgcttt tctgaatctg gtaacaacaa cagcgcgtcg
    18781 cccttttttt tcgcatgctt gtatgttctc gtaaacaatc ttgtgccgct caagtattgg
    18841 ttctaaaagg acgtcaacat tcccgccaca gagcataccc tcatcttcca cggcctttgc
    18901 gtccattctg gcgtggaaca attttacatt gttgctttcc atcaattcaa gggcctcacg
    18961 aataaccttc gcttcaagct cacccccgcc aattgtgcca taagtttttt catcctctcc
    19021 gacaagcatc ttggcgcctt cctctctagg agcagccccc tctttgctca ctactgtggc
    19081 aagtatgccc tttttcccct cgttaagata tttgaccaca caatcgtata tgctcatctt
    19141 gaacattcta tgcccgtctg ttaaattcgt ggagaattgc ttccaaaaca cctccaccta
    19201 tggctcttgc tttctccgta attgtgaaac aatactccgt aacacctctt ggatcaatat
    19261 cccctacctt ttcattctct atcacgttta tttcacggat gagccctctt aataacccgt
    19321 cgatgcttgc acggactggt acgtcatcga cgtagaggat gagctcacct tttttaacgg
    19381 tctctccaag ttccttcacg tgttttacaa gtccggcccg gggcgacctt aac
//
LOCUS       DAOOMC010000044        19431 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdus sp. isolate AMR_MDS_2685
            NODE_2827_length_19431_cov_6.671036, whole genome shotgun sequence.
ACCESSION   DAOOMC010000044 DAOOMC010000000
VERSION     DAOOMC010000044.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864760
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdus sp. (wastewater metagenome)
  ORGANISM  Syntrophorhabdus sp.
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae; Syntrophorhabdus.
REFERENCE   1  (bases 1 to 19431)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19431)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,729
            Genes (coding)                    :: 2,708
            CDSs (with protein)               :: 2,708
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 7 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19431
                     /organism="Syntrophorhabdus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2827_length_19431_cov_6.671036"
                     /isolate="AMR_MDS_2685"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2699756"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(137..415)
                     /locus_tag="PK125_06040"
     CDS             complement(137..415)
                     /locus_tag="PK125_06040"
                     /inference="COORDINATES: protein motif:HMM:NF017038.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HPB37702.1"
                     /translation="MKVEEKLALDAFKTDKESHITINQEICRTKCKEKNCIVVCPGHL
                     YSYNEENNEMVVEFAGCLECGTCKIACPEGAITWVYPKGDFGVQYRYG"
     gene            complement(423..1712)
                     /locus_tag="PK125_06045"
     CDS             complement(423..1712)
                     /locus_tag="PK125_06045"
                     /inference="COORDINATES: protein motif:HMM:NF013646.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HPB37703.1"
                     /translation="MDKIDVVIVGAGLSGLACAYKLAESDMQVIVVERGDFPGSKNVS
                     GGRLYTLPIRAMVGDMLKDAPFERKIVRERWSLLGDGNSVEVDLTSEKFRKEDHSFSI
                     LRATFDRWLADRLMEKGVFVIPKYRVDDLLWEQGMVAGVKAGQEEIYAHVVVAADGVL
                     SFMGEKAGLKPKMFPHNYALGIKEIIELPQEKIDDRFNVESGEGVAHLFLGDVTKGLF
                     GGGFLYTNKKSVSLGIVVGINALMESKQNIEAHALMDMFKERYELKTLIRDGNLSEYS
                     AHVIPEGGFGGISKLYGNGIILTGDAAGFALNMGVTVRGMEFAIASGIMAAETIIQAK
                     EASDFSEKSLASYEKKLKETFVLKDLETCKTMPAFLDNESFFNFYPKCFPDLLEKVMW
                     FDQMPKEGIGKTVWAHLKSSGMLNWKRLMELYRIKNL"
     gene            complement(1714..2694)
                     /locus_tag="PK125_06050"
     CDS             complement(1714..2694)
                     /locus_tag="PK125_06050"
                     /inference="COORDINATES: protein motif:HMM:NF013200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HPB37704.1"
                     /translation="MASDIMVFVEYKDGNIRKVSLELLTKGQELAKKDGATLYAVIIG
                     FQIQEVAESARAYADKTIIVDNESLNEYRYDTYVSVLEQLVKKYQPSFIFSGSTVTGK
                     DLFPRLAARLNVSIVSDAVGIDFSDNRLKVKKPLYGGKVISWLSFKEGVSPLITFRPN
                     SFSVEKLSLSGEIVEEKIDIFKDQKFNVLSVNKGGIEKVDLQEADFIICGGRGMKGPE
                     NFQVLEEIANIMGGRVGASRAVVDSKWREYDDQVGKSGKTVSPKLYIGCGVSGALHHT
                     MGMDTSKIIVAVNKDPNAPIFNYADYGIVDDLFNVLPLLKQELAKSKQDI"
     gene            complement(2707..3498)
                     /locus_tag="PK125_06055"
     CDS             complement(2707..3498)
                     /locus_tag="PK125_06055"
                     /inference="COORDINATES: protein motif:HMM:NF013200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HPB37705.1"
                     /translation="MNIIVFIKQVADTEARIIIKNDQKSLEIENKYNMNFFDEFAMEA
                     AIKTKEKFKDSKITACTYGPKRSIEALRTAIAMGADQAFLIDNTNLESDDSLIVAKIL
                     SAFARKEGFDLILCGRQAIDDENANVGVMVAEMLGIPHASSVTAFEVVDSRMVRADNE
                     IEGGIESIEVQLPALFTTQKGVNTPRVPLITGVMKAMKAVIPTIDPTTLGLSNEDIDL
                     NSSGIETISLEPPQQRPPVKMIDGETTEDKVRNLVKALKEEAKVL"
     gene            complement(3561..4709)
                     /locus_tag="PK125_06060"
     CDS             complement(3561..4709)
                     /locus_tag="PK125_06060"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HPB37706.1"
                     /translation="MNFALSEEQEFFKKTIADTVDRMIVPKAQEIDEKDEFPWDLWKE
                     FTGLGYIGLRYPEEIGGMNADKVMCMIFYEEVARGSVGFAQSVIMNILMGTYFIFRFG
                     SEAIKERCLYPAMRGEKVATMCFTEDQSGSDLGSTRTTAVKDGDGWRINGTKMWITNG
                     PVCDFCTVLATNDPSKGLKGLNFFLVEKGTPGFSSGQVLNKLGCRGTVTGELVFDDVW
                     VPDENFLGTELGKGVVYVGDILDEVRLMTGAMALGIARGAYAEALEFARKRMAFGQYI
                     GNFQLIREKFAEMDTLMNAARLMVYYGAWLLEENKDCRVLAAEAKMYATEVCLKVVDE
                     LTRIYGANAFAYEYSPQRYFRDARFLLYGGGTHEVLRDFIGRSLIGKL"
     gene            complement(4858..5616)
                     /gene="rpoD"
                     /locus_tag="PK125_06065"
     CDS             complement(4858..5616)
                     /gene="rpoD"
                     /locus_tag="PK125_06065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HPB37707.1"
                     /translation="MSKVEAAEKSCAAAKNELVKANLRLVVSIAKRYVNRGLSFLDLV
                     QEGNIGLMKAVDRFEYQRGYKFSTYATWWIRQSMARALADQARTIRIPVHMIETINRV
                     VKASRVLVQELGREPLPDEVAERVGFPVEKVRIVMKITREPISLETPINDDEDAYLLD
                     LIEDKSVITPHESALFDDLIEQVNKTLATLTPREEKVLRMRFGIGEKYDHTLEEVGQV
                     FEVTRERVRQIEARALRKLKHHSRTKRLRPFTDL"
     gene            complement(6389..8119)
                     /gene="dnaG"
                     /locus_tag="PK125_06070"
     CDS             complement(6389..8119)
                     /gene="dnaG"
                     /locus_tag="PK125_06070"
                     /inference="COORDINATES: protein motif:HMM:TIGR01391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HPB37708.1"
                     /translation="MKSTVEDLLARINILDIVSQHVKLRKTGKNFIGLCPFHKEKSPS
                     FTVSLEKQIYYCFGCHEGGNAINFLMKYENLSFQETLENLGKQYGVEVSRRSGEKRTS
                     TFEALSKLTDYYHQQLKNSKLAQQYLMRRGINEDSVEEFKIGFSDRSGQNIKSFLKKS
                     GISNDIFLSTGIIRIRNGEMYDMFGGRIIVPIFDVHRRTIGFGGRTIEKDALPKYINS
                     PESPVFSKRNALFGIEKTKNFIAEMDEAFIVEGYFDLIALYQHGFRNVVATLGTAITE
                     NQLSRLRNYSENITLMLDGDEAGIKSALRLIGIFSDMDIQGNMVVLPSGYDPDSFVRR
                     EGIEAVKGVIKDKKSILDYFFDYAKNKYGVNKLEGRISFIKAVMPHVETIQDGIKKRL
                     YIKRVSELTSIEEEHLWDGLREKGDRNPPDKYLSQSIIGKRVIGILLNNPDLIKLCKE
                     KSTLEYIKDISTREVLKHIVQYFEKEKELAMSSFVQSIEKEVLRELVLRAVLDATEYE
                     DIEDEKIAYDYFRHLEKKFIIDESQRITEKMAEAEKIGDEMEMMELLRRKRQVLNLMK
                     SHEMERDDYA"
     gene            complement(8116..10407)
                     /locus_tag="PK125_06075"
     CDS             complement(8116..10407)
                     /locus_tag="PK125_06075"
                     /inference="COORDINATES: protein motif:HMM:NF012700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HPB37709.1"
                     /translation="MTDKTLSYLDYLKLLDIIKNYSLTPFVKESISSLRPLSSLQSIQ
                     SRQDKIDAIIELVRWDGKLPLPDIPDIRDIMKRISIQDAILEEKSFLTLAGFMSACDN
                     LLDFLKNAFNKKLFIDEILQQLKPLTPVLRRIVKTINIEGFIEDTASYELSKIRSDLF
                     TFRERIRKRLEKIMEREGVKPILQDAYISLRNGRYVIPLKPNFNEVFQGIVHDYSHSL
                     KTSFVEPLEIVDLNNAINILEKDEKEEENKILRELTIFLRHNTEALEQNLDVIKEMDI
                     YHSLANFSIDFECIRPVVSENGLLEIKGARNPFISISKKTDTIPIDIFMESEQKTMII
                     SGPNAGGKTAALKTIGLLSLMAQSGFFIPASGNPVVPKFSNIFAIIGDEQDIAMELSS
                     FTAHMNTIKDLYTLAKGGELILIDEIGGNTEPQEASALAMGIIDAFVEKGCKVIVTTH
                     LNMLKAYGYTKPFAINIATAFDPDTMRPMYKLLYGTAGYSNAINVAKSIGVPKEIIDH
                     SYGYLGKQEYMLNDLIGALELGKSKVEGELEELRRMKDNLRKRLSVVKEQRDTYLGKV
                     EEKCQVKLNELESELEEIRKDLAKKERASLSRGKERLTRLQEKYRINFPRNREAISLG
                     DYVKIHSLGSTGHVVGVEKNGKTFEVVIGNVRTTVDSTLVAKISKPQGVSEKSTIQIH
                     AEKILTPEIRVIGMRVEEALKEIDRFLNRAIVQEMPRVKIVHGVGTGRLMNAIREHFK
                     DAAFISNIGKDDTNPGITIIELK"
     gene            complement(10428..10880)
                     /locus_tag="PK125_06080"
     CDS             complement(10428..10880)
                     /locus_tag="PK125_06080"
                     /inference="COORDINATES: protein motif:HMM:NF020977.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HPB37710.1"
                     /translation="MADFKEKLQDGLKEALKQKDHVRLSLYRMLLTSIKNKEVEKIRP
                     LTEDEFIAVVKTSIKQHVESIESFKKGDRADLAEREEKELSILKEFMPSQLSEDEVSR
                     EIEEAIVSLQVHGQKEMGKVIKFILEKYPGRIDGKVLSGMVLKRLSSK"
     gene            complement(10880..11089)
                     /gene="rpsU"
                     /locus_tag="PK125_06085"
     CDS             complement(10880..11089)
                     /gene="rpsU"
                     /locus_tag="PK125_06085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011235429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HPB37711.1"
                     /translation="MPAVVVRDGESFESALKRFKKLCEKTGILSEIKKREYFEKPSVK
                     RKKKMLAAKKRAIKKMRRTMDKGFY"
     gene            complement(11249..11965)
                     /locus_tag="PK125_06090"
     CDS             complement(11249..11965)
                     /locus_tag="PK125_06090"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HPB37712.1"
                     /translation="MRKRKRQEEHENAERWLLTYADLITLLLAFFIMMYVFSKQDAYK
                     YDEVASHLKTIFSGGMGLAGKGSVTATSPIGMPSRGASSGEIKRQLEGEFLDKNRNST
                     GSESISVLSDERGIVIRVLDKAFFDEGKAELKDGAKNALDRIVPIIKNVENHIRIEGH
                     TDNVPISTNEFKSNWELSVRRATEVVRYFVEKKGLPPERISATGYAEYRPIANNNSPQ
                     HRAMKRRIEIIVAKTKEKQN"
     gene            complement(11962..12729)
                     /locus_tag="PK125_06095"
     CDS             complement(11962..12729)
                     /locus_tag="PK125_06095"
                     /inference="COORDINATES: protein motif:HMM:NF006583.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein"
                     /protein_id="HPB37713.1"
                     /translation="MDITTIFGLLLGVSAVVIAFLMEGGHISSVLQAPAMIIVIFGTL
                     GASIVTTSFSQLTSLPKLIKVILFEKKLDPQELIDLIYELSQKSRKNGLLSLEKELDN
                     IKDPFLKKAIQLAIDGFETNKIREILEIEMSYIEERHKVGATFFQKLGGFSPTLGIIG
                     TVLGLIHALANMESSESMAQSIATAFIATLWGVALANLIYLPIADKLKAKHQHEALYL
                     EIISEGVISLAMGDNPRVIRMKLLSFLLPDKRPPEDR"
     gene            complement(12733..13323)
                     /locus_tag="PK125_06100"
     CDS             complement(12733..13323)
                     /locus_tag="PK125_06100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB37714.1"
                     /translation="MVDDLDDRREYFRIKDRLVIEARAISDEEFLQLDNKIRYNPTQI
                     IDRGCDMHFLKECISSDDKEKNQVLSYLVTINKKLDMLIDFLYKPEKDELYHSSHMEV
                     EVSGNGIKFFSDLEMQGGDYAELRIEIPRFPHPKITALAQVLKGEDCFVNGSKYREII
                     ARFIVINEEDRDLLIHYIFTKDRERLRLKKELFCGQ"
     gene            complement(13316..14776)
                     /locus_tag="PK125_06105"
     CDS             complement(13316..14776)
                     /locus_tag="PK125_06105"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HPB37715.1"
                     /translation="MIEGSKVLLVDDNVEIINILTDFLTLNGCKIYKASTGKEALEVL
                     SKADLEIVILDVKLPDVNGISLIDSIKIKNPTIGIIMITGYHDPNLIVEAMKKGASDF
                     LIKPFEFDKLILVLMRVVRERGFLMEKQKYIDTLEDKKKIELLNRELQRRIQELTTMY
                     HISNKFNSLNILDDVYDRMTQIINEVMDGRHCGYYIVDSENKELMLYKELGDGNNSFD
                     RRLTISDELIKEVRTTRKHHITDNKIFLPLVIKGENIGFVVVDSKGNGRYRNSFSDSD
                     IFFLKLVTEKASTQIENRMLYESLFEGVLHTLTSLITAINKRDLYTEDHCKRVTRSSL
                     ALGEMLKLDSYEKDVLRVVGPVHDVGKIGIPDSILLKPGKLTPEEYEIIKAHSVYGEE
                     IINRFEILSYEAKIIRHHHERFDGEGYPDRVARNEIPRGAKIIAVCDAYDAMVTDRPY
                     KKAYGKEEAINEIIRCKGTQFDPEIADCFVELVMHG"
     gene            complement(14817..15386)
                     /locus_tag="PK125_06110"
     CDS             complement(14817..15386)
                     /locus_tag="PK125_06110"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HPB37716.1"
                     /translation="MNNSDDAFRILIVDDNKELREILEEYLQSEAVHIEGAANGKEAL
                     VKYNRNPFDLIITDLNMPEITGMEMIKSIKQQNDVTEFIIITGYASLDSAVEAVKMGA
                     FDYIVKPFRMEELKVVVKNVRDKVALKKLNASLFNKLKGLYDEIERYRQKEENENTEP
                     TKTSMTDTEHILAKIKKIEEIAKGRLFIG"
     gene            complement(15373..15579)
                     /locus_tag="PK125_06115"
     CDS             complement(15373..15579)
                     /locus_tag="PK125_06115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB37717.1"
                     /translation="MTISDYQIGSVIQTYMKNMKIKVKENEGGLIQKPKNDEVSISRE
                     GMRKILYDRIGEQVIERSKKYEQQ"
     gene            complement(15633..15938)
                     /locus_tag="PK125_06120"
     CDS             complement(15633..15938)
                     /locus_tag="PK125_06120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB37718.1"
                     /translation="MKEFFRLTRNALDTDNDETFLHSLIQRNALFGALEQFSSCLSQG
                     FIEKMIFLEEMIIERLKTERKRMIKDIDEVSRKISTVKAYSALFPIPSMPAFFDLTG"
     gene            complement(15967..16794)
                     /locus_tag="PK125_06125"
     CDS             complement(15967..16794)
                     /locus_tag="PK125_06125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB37719.1"
                     /translation="MMKASIGQGILSIPPDRKICNQLKLSGAETEGSALRYDDSSFWQ
                     DTLRKMNNTIMRFMETIELINSAVSGKELPTNTDLEQILFSFAESMNGLLSACNRACN
                     ENDMATGNGSGGVQDNLLKLRGILISVLKMQESSGEEISVNLKSIGVKLINDASLVID
                     KRTVRDAISSRGGEVIKAVKTIVSSLFEAMPLCIDPNSGNLVNVRRKLEEKDGNHSKV
                     TAALTERLEKERCELMEKLRITELLIAHSNQFLDNLKLSAGFFPIIHSLYDSSGHGL"
     gene            complement(16794..17246)
                     /gene="flaF"
                     /locus_tag="PK125_06130"
     CDS             complement(16794..17246)
                     /gene="flaF"
                     /locus_tag="PK125_06130"
                     /inference="COORDINATES: protein motif:HMM:NF009435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0044781 - bacterial-type flagellum
                     organization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis regulator FlaF"
                     /protein_id="HPB37720.1"
                     /translation="MRENCSVAESLMMQTVRNPYDVYKKAEEKSLAGKDLEVAVLVKG
                     ARLLKECQRKWETYTQKQLLMELAEACKYNQRIWAIFQTEALQEDNPMPIQLKRNIIL
                     LAGYIDKRLLDVLAYPNPKKLTQIIDININIAAGLRGIPEGELPFDLD"
     gene            complement(17194..17607)
                     /locus_tag="PK125_06135"
     CDS             complement(17194..17607)
                     /locus_tag="PK125_06135"
                     /inference="COORDINATES: protein motif:HMM:NF019024.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis repressor FlbT"
                     /protein_id="HPB37721.1"
                     /translation="MALTISLRPKEQVVIGGAVLCNDEGRRTRLTIKNNVTILREKDV
                     LKPEDVDTPCKRLYFSIQLMYIDQENIAEYHKVYWKDAEDIVKAAPSCYENIEKISKF
                     VVAGDYYRALKQCKELIQYERKLLSCGIVNDANRA"
     gene            complement(17667..18248)
                     /locus_tag="PK125_06140"
     CDS             complement(17667..18248)
                     /locus_tag="PK125_06140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB37722.1"
                     /translation="MKVDGLDKRMHGDVFISPNTLKSRRTKTAPATATRVDRRDSANL
                     PEVFVRVDQAAAKIDQKLTIMTKILNQINSEKDDIIRARLARFFNVLKAFIDEALHPT
                     GDLATDRILAGKSVDIHIDNTSITLQGIRIKLEAQHSSLNELSRSPKTEDVHIMMQAI
                     RKAQRDVKKLRINLSSARDQITHHSKKTSSLTL"
     gene            complement(18322..19167)
                     /locus_tag="PK125_06145"
     CDS             complement(18322..19167)
                     /locus_tag="PK125_06145"
                     /inference="COORDINATES: protein motif:HMM:NF012874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin"
                     /protein_id="HPB37723.1"
                     /translation="MATISLTAQMTTNLLSLQNTAKLMGITQERLATGKKVNSAIDDP
                     VAYFDADAHYQLASDYATFKNGMSEAVQTIKTATDTIESIKNLLNQMKSLATSAKSAA
                     SQADAKNLWDQFGIIRAQVDHLADDAMYKGVNLLQSDTLNVVFNTKGDDLEVKGFNAH
                     ASGLSIASGSASMWGASGTSGAVAASQLANISAAIAAIDDAISTLRSQAKTLASNLSI
                     VTTRQDFTDKMIAILKVGGDNLTLADMNEEGANMLMLQTRQALGIQSLSMASQSAQQV
                     LSLFR"
BASE COUNT         4999 a         4556 c         3423 g         6453 t
ORIGIN      
        1 taaagaattg catcgggccc tgcgccagct gttttccgta ccgtttacaa tccggacagt
       61 cctgccccaa attgtggtaa cgtcacacat tcgatttcat tggctattcg ctgtcatctg
      121 acagcgaata gccccattat ccatacctgt attgaacgcc aaagtcgccc ttggggtaca
      181 cccaggttat tgctccctca gggcaagcaa ttttgcaggt accacactcc aggcaacccg
      241 caaactccac aaccatctca ttattttctt cattgtatga gtacagatgt ccaggacaga
      301 ctacaatgca atttttttct ttacattttg tccggcatat ttcttgatta atggtaatat
      361 gactttcctt gtctgtttta aatgcatcaa gggcaagttt ttcttcaact ttcatagggt
      421 cctcaaaggt tctttatcct gtataactcc atgagtcttt tccaattcaa catgccagat
      481 gattttaaat gcgcccatac tgttttcccg ataccttcct ttggcatttg atcaaaccac
      541 atgacctttt ccagaagatc cggaaaacat ttggggtaaa agttaaaaaa tgattcattg
      601 tcaagaaaag caggcatggt tttacatgtc tcgagatctt tcagcacaaa tgtttctttg
      661 agtttctttt cataactggc cagagatttt tctgaaaaat cactggcttc ctttgcctga
      721 ataattgttt ctgctgccat gatgcctgac gctatggcga attccatccc ccttactgtc
      781 acacccatat ttagggcaaa tccagccgca tcccccgtta aaataatacc gtttccgtac
      841 aactttgaaa ttccaccaaa accaccctct ggtattacat gggcagaata ttctgaaaga
      901 tttccgtctc taatcaaagt tttcaactca taacgttctt tgaacatatc cataagggca
      961 tgggcttcaa tattctgttt gctttccata agggcattta ttcccaccac aatccccaaa
     1021 gaaacagatt ttttattcgt atataaaaaa cccccaccaa aaagtccttt ggttacgtca
     1081 cccaggaata gatgagctac cccctcaccc gattcaacat taaacctgtc atcgatcttc
     1141 tcctgcggaa gctcaataat ctctttaata cccaaagcat agttgtgagg aaacatcttt
     1201 ggcttaagtc cagccttttc tcccatgaaa gatagtaccc catcagcagc taccaccaca
     1261 tgtgcataaa tctcttcctg accggcttta acccctgcta ccatgccttg ttcccacagg
     1321 agatcatcga ccctgtattt tggtataaca aaaacgccct tctccatcaa tctatctgca
     1381 agccacctat caaacgtagc acgaaggatg gaaaagctgt gatcctcttt tctgaatttt
     1441 tcgcttgtaa ggtctacttc gactgaatta ccatctccga ggaggctcca ccgttcccgt
     1501 acaattttcc tttcaaatgg agcatccttg agcatgtccc caaccatggc cctgatagga
     1561 agagtataga gcctgccacc tgacacgttt tttgagccag gaaaatctcc acgttcaacg
     1621 acaattacct gcatatcact ttctgcaagt ttatatgcac aggcaagacc tgacagtccc
     1681 gcgcctacaa tgacaacatc aattttgtcc atgtcagata tcttgttttg attttgctaa
     1741 ttcttgcttc aataaaggta aaacgttgaa taaatcatca acgataccat agtctgcgta
     1801 attaaaaatg ggcgcatttg gatccttgtt cacagctact attatttttg acgtatccat
     1861 gcccatagta tgatgtaagg caccagatac tccacagcct atatagagtt ttggtgatac
     1921 tgttttgcca ctttttccaa cctgatcgtc atattcacgc catttactat cgaccactgc
     1981 cctagatgca cctactctgc cgcccatgat atttgcaatc tcttcgagga cttgaaaatt
     2041 ttcaggtcct ttcatgcctc ttcctccgca aatgataaaa tcggcctctt ggaggtcgac
     2101 tttctcaata ccccctttat ttacgctgag cacattaaat ttctgatctt tgaagatgtc
     2161 gattttttcc tcaacaatct ctccgctcag ggaaagcttt tctactgaaa aggaatttgg
     2221 tctgaatgta atcaagggag agacaccttc tttgaaagat agccacgata ttaccttgcc
     2281 accgtaaagg ggttttttta ctttcagacg gttatcagaa aaatctatac ctactgcatc
     2341 agatacaata gaaacattga gtcgcgccgc aagccttgga aacaaatcct tgccagtgac
     2401 tgttgagcca ctgaaaataa atgacggttg atatttcttc acaagctgtt caaggacaga
     2461 cacgtacgtg tcgtacctgt attcattcag gctttcattg tccacaatga ttgttttgtc
     2521 tgcgtatgcc ctcgcagact cagcaacttc ttgtatttga aatccaatga tcacagcata
     2581 taaggttgca ccgtcctttt ttgccaattc ttgacctttt gtgagaagtt ctagggaaac
     2641 cttccttatg ttgccatcct tatattccac aaaaaccatt atgtcacttg ccatcgttct
     2701 ttcctcctac aaaacctttg cttcttcttt cagggcttta acaaggtttc gcaccttgtc
     2761 ttctgttgtt tcaccatcaa tcatttttac cggtggtctc tgttgtggag gttccagaga
     2821 gattgtttcg atacccgatg agttcagatc tatatcttca ttcgataacc cgagcgtagt
     2881 cggatcgatg gtgggtatga ccgctttcat tgctttcatt acgccagtga tcagaggtac
     2941 ccggggtgta ttcacgcctt tctgagttgt aaaaagggcc ggcaactgta cttcaatgct
     3001 ctcaatgcct ccctcaatct cgttatctgc tctcaccatt ctactatcaa caacctcgaa
     3061 tgccgtaaca gaactggcgt ggggaattcc aagcatttcg gcaaccataa cacccacatt
     3121 agcattctcg tcgtcaatgg cctgtctgcc gcacaaaata aggtcaaaac cctcttttcg
     3181 tgcaaaagca gaaagaatct ttgccacaat gagcgagtca tcactctcaa gattagtgtt
     3241 atcaattagg aaagcctggt cagcccccat tgcgatcgct gttcgcaggg cctctataga
     3301 ccttttcggg ccatacgtac aggcagtaat cttgctatcc ttaaacttct ccttggtttt
     3361 gatcgctgct tccatggcaa attcatcaaa gaagttcatg ttgtacttat tttcaatttc
     3421 cagcgacttt tgatcgtttt ttattatgat tcgtgcttca gtatctgcta cctgttttat
     3481 aaatacgata atgttcacaa tcccgacctc cttatccagc aattacagaa tcattaggat
     3541 ttatagaaaa atgctgattc ttaaagtttt cctatgaggc ttcttccgat aaaatcccgc
     3601 aaaacctcat gggtgcctcc tccgtacaag agaaaccgcg cgtccctaaa atatctctgt
     3661 ggagaatatt cataggcaaa ggcatttgcc ccataaatcc ttgtcagttc atcgactact
     3721 ttcaaacaga cttcggttgc atacatcttt gcctctgcag ccagaaccct gcaatccttg
     3781 ttttcctcga gaagccatgc gccgtagtat accataagtc gagctgcatt cataagggtg
     3841 tccatctcag caaacttttc tctaatgagt tggaagtttc caatgtattg tccaaaggcc
     3901 atcctctttc ttgcaaactc aagggcttca gcgtatgctc cacgggctat cccaagggcc
     3961 atggcacctg tcataagtct tacttcatct aagatatcac caacatagac cacgcctttc
     4021 ccgagttctg tacccagaaa gttttcgtct ggtacccata catcatcaaa gacgagttca
     4081 cctgttacag tccccctgca accgagtttg ttcagtacct gaccactcga aaaaccgggc
     4141 gttccctttt caacaaggaa aaaattcaag cccttgagtc cttttgatgg atcattcgtt
     4201 gcaaggacag tgcagaaatc acaaacagga ccgtttgtga tccacatctt cgttccatta
     4261 atccgccatc catcaccatc tttgacagca gtcgttctcg tggaaccaag gtctgaccct
     4321 gactggtcct cagtgaaaca catggttgct actttttcac ctctcattgc tggatagaga
     4381 catctttctt taatcgcctc actcccgaat ctgaagatga aatatgtgcc catgaggata
     4441 ttcataatca cggattgggc aaaaccaaca gaacctctcg caacctcctc ataaaaaatc
     4501 atacacatga ctttgtcagc gttcataccc cctatctctt ctggataacg taaaccaatg
     4561 tacccaagtc cagtaaattc tttccagagg tcccatggaa actcatcttt ttcatctatc
     4621 tcctgagctt tcggaacaat catcctatct accgtatcag caatggtttt cttaaaaaat
     4681 tcctgctctt ccgataatgc aaagttcatt ttataaccct ccccgaagaa aaaaataagg
     4741 atattttgta tacaatattt tactgaccta tttttgtcaa gggaaattct gtctgtgtat
     4801 tgggtagatt agggaatgat gagacctgag tacagatcaa aaggatgata aaaatttcta
     4861 caaatcagtg aacggcctta atcgttttgt tcgtgaatgg tgttttagct ttcttaatgc
     4921 ccttgcttca atctgcctga ccctttcccg tgttacttca aatacctgtc caacctcttc
     4981 gagagtatgg tcgtatttct ctcctattcc aaatcgcatt ctcaacacct tttcctcacg
     5041 aggcgttaac gtagcaagtg ttttattgac ctgttcgatc aggtcatcaa ataatgctga
     5101 ttcatgagga gtaataacac ttttgtcctc aataagatca agcaaatagg catcttcatc
     5161 atcattaatg ggcgtttcta aagaaatcgg ctctcgtgta atcttcataa ctatccgaac
     5221 cttctctact ggaaatccca ctctctcggc aacctcatca ggcaaaggct ctcttcccaa
     5281 ttcctgaaca agtacccgtg acgccttaac aactctattg atggtctcta tcatgtgaac
     5341 aggtatcctt attgttcgcg cctgatctgc tagagctctc gccatggatt gtctaatcca
     5401 ccacgttgcg taggtgctga atttgtagcc cctttgatac tcaaaacgat cgactgcctt
     5461 cataaggcct atatttcctt cctggactaa atcaagaaat gataaacctc tatttacgta
     5521 tctcttggca atgcttacaa cgagtctcag attcgcctta actaactcat tttttgctgc
     5581 ggcgcagctc ttttctgctg cctcgacctt tgacatataa ctttttagtt catctacgct
     5641 gataccaatc tcattttcaa ttttcttgat ttttttggga attactgaca aaagtttctt
     5701 gtttccgccg tttataatgc ttttggtctc cttagattct tcttcaagtt tttccacttt
     5761 ttcccggtag cgcttcatcc ttttgactat tctctctatt atcttcttac tcgggtttat
     5821 ttcaaggata agtgcttcaa tctgcttgag gtatttattt ttatttcttc cctcgctcgt
     5881 tgcataactc tttattaaaa catccaattt atcagaaagc tctgccagac gattcttttg
     5941 gttttcgagt atttgatcat cctcctcatc ttcgtcaact gtatctgtta cgtctgaaac
     6001 ctttgcccgt gatgccctca tgttttcaag ggcctggtgt agttcacaaa atgtctgagg
     6061 gaataagatt atgattcgtt ttatttcttc tctcgattgc tcaatcctga ctgcaatctc
     6121 tttctcaccc tcccgggtta gcaatgccat accaaccatc tctttaatat aatgtttctc
     6181 aggatctgga agaaccggat caagggatga ttgattctca tcatgtaaaa gttcatcaat
     6241 gccaccccca tcatccaaca ccatttcatc atcagggtca gggctcagga attcaatttc
     6301 atccatatca tccttttcga ggctatctgc gaatttatca ccaaatccat cgtcatcaag
     6361 tgggatttga aagatatctt tgatcttctc aggcataatc gtctctctcc atttcatgtg
     6421 acttcatgag gtttaagacc tgtcttttcc gtcgaagaag ttccatcatt tccatttcat
     6481 cgccaatttt ttcagcctcg gccattttct cggtaattct ttgactttca tcaataataa
     6541 atttcttctc caagtgcctg aagtaatcgt aggcaatctt ttcgtcttca atatcctcat
     6601 actctgtggc atccaaaaca gccctgagaa cgagttctct caaaacctcc ttttcaatag
     6661 actgtacaaa acttgacatt gccagttcct tttctttttc gaaatactgg acaatatgtt
     6721 tcaaaacttc cctcgtggat atatccttaa tgtattcaag tgtgcttttt tccttgcaca
     6781 actttataag atcaggatta ttgagtaata ttcctataac gcgtttgcct atgatgctct
     6841 gggacaaata cttatcagga gggtttctat cccctttttc tcttaaccca tcccataaat
     6901 gctcttcttc gatactcgtg agctctgata ctctttttat atataatctc tttttaattc
     6961 catcctgtat tgtttctacg tgaggcatga cagcttttat gaaggagatc cttccttcaa
     7021 gtttattgac tccatatttg tttttcgcat aatcgaagaa ataatcaagt atggatttct
     7081 tatctttgat gaccccttta acagcttcaa tgccttctct ccttacaaaa ctatccggat
     7141 cgtacccaga gggaagtaca accatgttac cttgtatatc catatcagaa aaaataccaa
     7201 taagtcttag ggcacttttt atccccgctt catctccatc tagcatgagt gttatgttct
     7261 cagagtaatt tctcaatctt gaaagttgat tctctgttat cgctgtgccc agagtcgcta
     7321 caacatttct aaaaccgtgt tgataaaggg caattaaatc aaaataaccc tctacaataa
     7381 acgcctcgtc catttcagca ataaaattct ttgttttttc tataccaaac aatgcatttc
     7441 tttttgaaaa aactggtgat tcgggtgagt ttatatattt tggtaaggca tccttttcaa
     7501 tagtccttcc tccgaaacca attgtacgcc tatgaacatc gaatataggt acaataatcc
     7561 gcccaccgaa catatcatac atttccccat tcctgatacg aataatgcct gtgcttaaaa
     7621 aaatatcgtt ggagattcct gattttttca gaaagctttt tatgttttgc cctgaccgat
     7681 cactaaaccc aattttaaac tcttcaacag aatcttcatt aattcctctt ctcatgaggt
     7741 actgttgggc caattttgaa tttttcagtt gttgatgata gtagtctgtc agttttgaca
     7801 gtgcttcaaa agtgcttgtt cttttttcgc ctgaccgacg cgaaacttcg acaccatact
     7861 gcttcccaag gttttccagg gtttcctgaa acgagagatt ttcatacttc attagaaagt
     7921 tgatcgcatt tccgccttca tgacatccaa aacaataata tatttgtttt tctaaactta
     7981 cagtaaatga tggagatttt tctttatgaa atggacataa ccctataaaa ttttttccag
     8041 tttttcttaa tttaacatgt tgtgaaacaa tatcgagtat gttaattcgt gcaagaagat
     8101 cttcaacagt agatttcatt taagctcgat gatggttatg cctgggttgg tgtcatcttt
     8161 tccgatatta ctgatgaaag ctgcatcttt gaagtgttcc cttatggcat tcattaatct
     8221 tcccgtgcct actccatgca caatttttac ccggggcatc tcctggacaa ttgcacggtt
     8281 cagaaaccta tcaatctctt tcagcgcctc ctctaccctc attcctatta ctctgatttc
     8341 cggggtaagg attttttctg cgtgaatctg tatagtgctt ttttcactca ccccttgtgg
     8401 cttggagatt ttcgcaacaa gtgtgctatc aacagtagtt ctcacattac caataactac
     8461 ttcgaaggtt ttgccatttt tttccactcc cacaacatgg cctgtacttc cgagtgaatg
     8521 aatctttaca taatcaccca aactaatggc ttccctgttt cttggaaagt ttattctata
     8581 tttctcctgt aatcgggtca gtctttcttt accacggctc agtgatgccc gttctttttt
     8641 tgcaagatct ttcctgattt cctccagttc actctctaat tcattcagtt ttacctggca
     8701 cttctcctcc actttcccaa gatatgtatc tcgttgttct ttaaccacgg acagtctctt
     8761 ccttagattg tccttcatcc tccttagttc ttccaattcc ccttcaacct tacttttccc
     8821 aagttccaga gcgccaatga gatcattcag catgtactcc tgttttccga ggtacccgta
     8881 actgtggtct atgatctctt ttggtacacc aatgcttttt gccacgttga tggcattgct
     8941 gtatcctgca gtgccgtaaa ggagtttata cataggcctc atcgtatccg ggtcaaaagc
     9001 cgttgcaatg tttatcgcaa aaggctttgt gtacccatag gctttcaaca tgttcaaatg
     9061 tgttgtaaca atgaccttgc accccttttc aacaaaggca tcgatgatgc ccatagcaag
     9121 tgccgatgcc tcttgcggct ctgtattgcc acctatttca tcaatcaata taagttcccc
     9181 acctttcgcc aatgtgtaga gatctttgat ggtattcatg tgggcggtaa aactgctgag
     9241 ttccattgca atatcttgct catcgccaat aatagcaaat atattggaga attttggtac
     9301 tacgggattc cctgaggcag gaataaaaaa accggattgc gccattaggg aaagaagccc
     9361 aatggttttc aatgctgcag tctttcctcc agcatttggg ccactaatga tcattgtttt
     9421 ctgttcactt tccataaata tatcaatagg aattgtatct gtctttttgc taatcgagat
     9481 aaacggattt cgtgcccctt ttatctcaag aagtccgttt tctgaaacaa ccgggcgtat
     9541 gcattcaaaa tcgatgctga agttagccag tgaatggtaa atatccatct cctttatcac
     9601 atcaagattc tgttccaatg cctcggtatt atgccgtaaa aatattgtca attctcttag
     9661 gattttattc tcttcttctt tttcatcttt ttccaatata tttatagcgt tgtttaagtc
     9721 cactatttca aggggctcca caaaagacgt cttcagagaa tgggagtaat cgtgaacaat
     9781 accctgaaac acttcattaa agtttggctt aaggggtata acgtacctgc cgtttctgag
     9841 ggagatgtag gcatcctgga ggataggttt cacaccttct ctttccataa tcttttcaag
     9901 ccttttcctg atacgttccc ggaatgtaaa aaggtcagat cgaattttgg acagctcata
     9961 tgatgcagtg tcttcaatga acccttcaat attaattgtt tttacaatac gccgaaggac
    10021 aggtgtaagt ggctttaact gttgaaggat ttcatcgata aacagctttt tgttaaatgc
    10081 atttttcaag aaatccagca gattatcaca ggcgctcata aacccggcaa gggttaaaaa
    10141 tgatttctct tcaagaatgg catcttgaat agaaattctt ttcatgatat ccctgatatc
    10201 aggaatatct gggaggggaa gtttaccgtc ccatcttaca agttcaataa tggcatcgat
    10261 tttgtcctgt cttgattgaa tgctctgaag tgagctgaga ggcctgagcg atgaaataga
    10321 ctcttttaca aaaggggtta aggaatagtt ttttataatg tcgagaagct taagataatc
    10381 gagatatgat aaggtcttgt cggtcatatg agggcatgat cgactatcta cttagaggac
    10441 aatctcttaa gcaccattcc gctcaacact tttccgtcta tcctccctgg atatttctca
    10501 agaataaatt ttatgacctt ccccatctct ttctgtccgt gtacctgcaa agatacaatc
    10561 gcttcttcaa tttctctgct gacttcatct tcagataatt gggaaggcat aaactctttc
    10621 agtattgaaa gttctttttc ctctctctct gcgaggtctg ccctgtcccc ttttttgaag
    10681 ctctctatgg attccacatg ctgttttatt gatgttttaa caacagcaat aaattcatct
    10741 tctgtaagag gtcttatttt ttctacttct ttgttcttta tcgatgtgag aagcattctg
    10801 taaagagaca atcgaacatg gtctttctgt ttaagtgcct ctttcaagcc atcctgcagt
    10861 ttctctttga aatcagccat caataaaaac ccttgtccat agttcttctc atctttttta
    10921 tggccctttt ttttgccgca agcatcttct tttttctttt aacgcttggc ttctcgaaat
    10981 attctctttt ctttatttct gaaagaatac ctgttttttc gcacagtttt ttgaaacgtt
    11041 taagggcact ctcaaaagac tctccgtctc ttacaacgac tgctggcatc cattcttccc
    11101 tccctccgtg gacaactgaa aattttaagt gacgttatta gaactcaaaa aacggtcaaa
    11161 gtcaatatct ttatagactc ttacacaaca tgaataggcc gtcataacgg ttgattctgt
    11221 tggatgtggt aaataatcac atacgggatc aattctgttt ttcttttgtc ttggccacaa
    11281 tgatttctat gcgtctcttc attgctcgat gctgtggaga attattgttt gcaataggcc
    11341 gatattctgc ataccctgta gctgagattc tctccggcgg caaacctttt ttctcgacaa
    11401 aatatcggac cacttcggtt gcccttctta ctgaaagttc ccagtttgat ttaaattcat
    11461 tcgtactgat tgggacatta tctgtgtggc cttcaatcct gatatgattt tcaacgtttt
    11521 tgatgatagg cacaatcctg tcaagggcat tttttgcccc atctttaagt tctgccttgc
    11581 cttcatcaaa gaacgccttg tcaagcactc gtattacaat tccccgttcg tcagaaagaa
    11641 ctgatatact ctctgacccg gtactgttac ggtttttgtc caggaactca ccttcaagct
    11701 gtcttttaat ttcaccgctt gaagcccctc gcgagggcat tcctatggga gaagtagcag
    11761 tcacacttcc ttttcctgcc agacccatgc cacctgaaaa aatggttttt aggtggcttg
    11821 ccacctcgtc gtatttgtaa gcgtcctgtt ttgaaaatac gtacatcatt atgaaaaaag
    11881 caagaagaag ggtgatcaga tcggcatacg taagaagcca acgttcagca ttttcatgtt
    11941 cttcttggcg ttttcgtttt ctcatctatc ctcgggaggc ctcttgtcag gcagaaggaa
    12001 ggagagtagt ttcatccgta taaccctggg attgtctccc atggcaagag aaatgactcc
    12061 ttcactgata atttcgagat agagggcttc gtgttgatgt ttagccttca gtttatcagc
    12121 aatgggcagg tagatcagat tggccagtgc tacaccccac agcgttgcga taaaagctgt
    12181 tgcaatagac tgtgccatac tttcactact ttccatatta gcaagcgcat gtataagacc
    12241 aagaaccgtc ccaatgatgc cgagggttgg tgaaaaacct cccagcttct ggaaaaacgt
    12301 tgcccccacc ttgtgtcttt cttcaatgta tgacatctca atctcaagaa tctcacggat
    12361 cttattcgtt tcaaagccat cgatagcaag ctgtatggct ttcttcagga aaggatcctt
    12421 gatattgtct aactcctttt ccaagctcag gagaccattc ttccttgatt tttgagaaag
    12481 ctcgtaaatt agatctatca actcttgtgg gtcgagtttt ttttcaaaca atatgacctt
    12541 gatgagtttg ggcaaggatg tgagctgact gaaggatgtt gtaacaatag aagcgcctaa
    12601 ggtgccaaat atgacaataa tcatagccgg tgcctggagc acagaagata tatgacctcc
    12661 ctccataaga aaagcgataa cgactgcgct cactcccaac aataggccaa atatggttgt
    12721 tatatccata ggttattgtc cgcaaaaaag ctcttttttc aaacgcaagc gctccctgtc
    12781 ttttgtgaag atatagtgaa tgagaagatc cctgtcttcc tcattgatca cgatgaaccg
    12841 tgcgattatt tcccggtatt tacttccgtt cacaaagcaa tcctctcctt tgagtacctg
    12901 ggcaagggca gttattttag gatggggaaa gcggggtatt tcaattcgca gctcagcata
    12961 gtcccctccc tgcatctcca ggtccgagaa aaacttgata ccgttaccgc ttacttcgac
    13021 ttccatatgg ctcgaatgat aaagctcatc tttttcaggt ttgtaaagaa aatcgatcaa
    13081 catatcaagt ttcttattga ttgtgaccaa gtaagaaaga acttgattct tttctttgtc
    13141 gtcgctcgaa atacattcct tcaagaagtg catatcacaa cctctatcaa ttatctgcgt
    13201 cgggttgtat cgtatcttgt tgtcaagctg caggaattct tcatcgctga tagccctggc
    13261 ttcaatgact aagcgatctt ttatcctgaa atattccctt ctatcatcca aatcgtcaac
    13321 catgcatcac cagctcaaca aagcagtcgg caatctcagg gtcaaactga gtgcctttgc
    13381 accggattat ttcatttatt gcttcctctt tgccataggc tttcttgtat ggcctatctg
    13441 taaccattgc atcatatgca tcgcatactg caatgatttt agccccccgt ggtatttcgt
    13501 ttctcgcaac cctgtcggga tacccttccc catcgaacct ttcatgatga tgccgtatta
    13561 tttttgcttc gtaagagaga atttcaaagc ggtttattat ttcttcacca tagacagaat
    13621 gggccttgat tatttcgtac tcttcgggag tcaacttgcc tggcttgagt aaaattgaat
    13681 cgggtatacc aatcttccca acatcatgaa cagggccaac gacccttaac acgtcttttt
    13741 cgtatgaatc tagttttagc atttcaccga gcgcaaggct cgaccgggtt accctcttgc
    13801 aatggtcctc cgtatataaa tctcgcttgt tgattgccgt aataagcgac gtcaatgtat
    13861 ggaggacccc ttcaaaaagg ctttcataga gcatcctatt ctcaatctgg gtcgatgcct
    13921 tttctgttac taattttaga aaaaatatgt cactgtctga aaaactgttt ctgtatctac
    13981 catttccttt gctgtccaca acaacaaatc ctatgttttc tcctttgatt accaagggaa
    14041 gaaaaatttt gttatccgta atatggtgtt ttcttgttgt gcgtacctct ttgatcagtt
    14101 catcagagat tgtcagcctt ctatcaaagc tattatttcc gtctcccaat tctttgtaaa
    14161 gcatcagttc tttgttctcg ctgtcgacga tgtagtatcc gcagtgtctt ccatccatga
    14221 cctcattaat aatctgggtc atcctgtcat aaacatcatc gaggatatta agggaattaa
    14281 atttattgga tatatgatac attgtcgtca gttcttgaat tcgtctctgc aattctctgt
    14341 tgagaagttc aatcttcttc ttatcttcaa gggtatcgat atacttttgt ttctccataa
    14401 gaaaacctcg ttcacgaaca accctcatca gaacaaggat aagcttgtca aattcaaacg
    14461 gctttatgag gaaatctgac gcaccctttt tcattgcctc cacgatgaga ttcggatcat
    14521 ggtaaccggt aatcataata atgccaatcg ttggattctt gatctttata gaatcaatca
    14581 gagatattcc gttaacatcg ggaagtttga cgtccaagat tacaatctcc aaatcagctt
    14641 tgctaagaac ttctagcgct tctttgcctg tagatgcctt atatatcttg caaccgttca
    14701 atgtcagaaa atcagttaat atattaatga tctctacgtt atcatcaacg agaagaacct
    14761 tggaaccctc aatcattcag aaaacctctc tacaacattt tcttttcctt gtaacatcag
    14821 ccaatgaaca accgtccttt tgcgatttct tcgattttct ttatttttgc gagaatatgc
    14881 tctgtatctg tcatacttgt ttttgtcggt tctgtgttct cgttttcctc tttttgtcta
    14941 tatctttcaa tctcatcata aagccctttt aatttattaa acaacgaagc attgagtttc
    15001 tttaatgcca ccttgtctct cacgttcttg acaacaactt tcaactcttc catacggaat
    15061 ggtttcacaa tataatcgaa agcccccatc tttacagctt caaccgcaga atccagtgat
    15121 gcataacctg ttataatgat gaactctgta acatcgtttt gctgtttgat gcttttgatc
    15181 atttccatac cggtaatttc cggcatattc aggtcagtta taataaggtc aaagggattc
    15241 cggttatact tgacgagagc ttctttaccg tttgctgcac cttcaatatg cacagcttca
    15301 ctctgcagat actcttccag tatctctcgg agttctttgt tatcatcaac aattaggatt
    15361 ctaaacgcat catcactgtt gttcatattt tttcgacctt tctatcacct gttcgccaat
    15421 ccgatcatag aggatttttc tcatcccctc tctggatata gacacttcat cattcttcgg
    15481 cttctgtatc agaccacctt cattttcctt tacctttatc ttcatgttct tcatgtaggt
    15541 ttgtatgaca ctgccgattt gatagtccga tatcgtcata gttgtcctta tatgacttat
    15601 cggagtggaa ttttgaaaac ttaagatcgt ctttagcccg tcagatcgaa aaaagcgggc
    15661 attgaaggga tgggaaacaa tgctgaatat gcctttacag tgcttatctt tcgcgagacc
    15721 tcatcgatgt cctttatcat tctttttctt tcagttttta gcctttcgat tatcatttcc
    15781 tctagaaaaa tcatcttctc aataaatcct tggctcaggc aggaggagaa ctgttctagc
    15841 gccccaaata acgcattcct ttgtatcaga ctatgaagga acgtttcatc gttatcagta
    15901 tccagggcat ttctcgtgag cctaaaaaac tccttcatat ttatgcccct gcttgatatt
    15961 tgtctttcat aaaccatggc ctgaactatc atataaacta tgaataatgg ggaagaatcc
    16021 tgccgaaagt ttcagattat ccagaaactg attggagtgg gctataagta attcagtgat
    16081 tctcaacttt tccatgagtt cgcacctttc tttttcaagc ctctcagtca gcgctgcagt
    16141 gacctttgag tgattcccat ccttttcttc cagctttctt ctcacattta ccagattccc
    16201 cgagtttgga tcaatacaaa ggggcatcgc ctcaaaaagg gaggaaacaa ttgtcttaac
    16261 agcttttatg acttctccac ctcttgaaga tattgcatcc ctcaccgttc gcttatcaat
    16321 tactaacgat gcatcgttaa tcaatttgac cccgatcgac tttaaattca cagatatttc
    16381 ctcaccggaa gattcctgca ttttcaacac agagataagg atgcctcgga gttttaacaa
    16441 attgtcttgc acgccaccac taccattgcc tgtggccata tcattttcat tgcatgctct
    16501 attgcacgcg ctcaataatc cattcatgct ctctgcaaat gaaaagagta tttgttccaa
    16561 atcggtattt gtcggtaatt cctttcctga cacagcgcta tttatcagct caatagtttc
    16621 catgaacctc attatagtat tattcatttt ccgcaaggta tcctgccaga atgaagaatc
    16681 gtcataacgt aaggccgagc cttcggtttc agcacctgac agtttcagtt gattacagat
    16741 tttacgatct ggagggattg aaaggatacc ctggcctatt gatgctttca tcattaatcc
    16801 aggtcgaagg gaagctctcc ttctggaatt cctctaagtc ctgcagctat atttatattg
    16861 atatcaatta tctgtgtcag ttttttaggg ttaggatacg ccaatacatc aagaagtctt
    16921 ttgtcaatat atccagcaag tagaattatg ttacgtttga gttggatagg catcggatta
    16981 tcttcctgta aagcttctgt ttgaaagata gcccagattc tctggttgta tttacatgct
    17041 tctgccagct ccatcagcag ttgtttctgc gtatatgttt cccactttct ctgacattcc
    17101 ttgaggagac gggcgccttt aacgagcaca gccacttcca ggtctttgcc ggccaagctc
    17161 ttttcctctg cctttttgta gacatcatag gggttacgca cggtttgcat cattaacgat
    17221 tccgcaactg agcagttttc tctcatactg tattagctcc ttacattgtt taagcgctct
    17281 ataataatca ccggcaacca caaacttgct tatcttttca atgttctcat aacaactcgg
    17341 ggcagccttt actatatctt cggcatcttt ccagtatact ttatggtatt cagctatatt
    17401 ttcctgatct atatacataa gctgaatact aaagtagagc ctcttgcatg gcgtgtctac
    17461 atcctctggc ttaagaacat ctttttctcg taaaatagtt acattgtttt taatggtaag
    17521 gcgagttctc ctgccttcat cattacacaa tacggcacca ccaattacaa cctgctcttt
    17581 tggtctcaat gatattgtta aagccatagt tcaccctccc gtgccgtggt ttttcatgta
    17641 ataactggtg cacataatgt gccaatttat agagtgaggc tggaagtttt ttttgaatgg
    17701 tgtgttatct gatccctcgc tgaagaaagg ttgattctca gttttttcac atctctctga
    17761 gctttgcgaa tagcctgcat cataatatgc acgtcttcag tctttggtga tcttgataat
    17821 tcatttaagc ttgaatgctg tgcttctaac tttattcgta tcccttgaag ggtaatagag
    17881 gtattgtcaa tatgaatgtc aacgcttttt cctgcaagaa tacggtcagt tgccaagtca
    17941 cctgtaggat gaagagcctc gtcaataaac gccttcaaaa cattaaagaa acgagcaaga
    18001 cgcgcacgga tgatgtcatc tttttctgag tttatctggt tgagaatttt tgtcattatt
    18061 gtcaattttt gatctatctt tgcggctgcc tgatctacac gtacaaagac ttcgggcaga
    18121 ttggcactat cccttctgtc cacgcgggtt gctgttgcag gtgctgtttt tgtccgcctt
    18181 gatttcaatg tattgggaga aatgaaaaca tcaccatgca ttctcttgtc cagtccatct
    18241 accttcatga tgtattaatc acccttatga cattgcataa aagggagggg ctgttttgcc
    18301 cctcccgggt ttggaatgtt tttacctgaa gagtgaaaga acctgctgtg cagactggga
    18361 agccatactc agtgactgaa taccgagtgc ctgccgtgtc tggagcatca gcatgttcgc
    18421 accttcctca ttcatgtcgg caagggtcag gttgtcgccg ccgaccttta ggatcgcgat
    18481 cattttatcc gtgaaatcct gtcttgttgt cacaatactc aggttggatg caagtgtctt
    18541 tgcctgagat ctcagggtag aaattgcatc atcgattgca gcaatcgccg cgcttatatt
    18601 ggcaagctga gaagctgcca cagcgcctga tgtaccggat gcaccccaca tagacgctga
    18661 tccgctagct atcgagaggc cactggcatg ggcattgaat cctttcacct caagatcatc
    18721 gcctttggtg ttaaatacga cattcaacgt atcgctctgt aaaaggttta cacctttgta
    18781 catcgcgtca tccgcgagat ggtcaacctg ggccctaatg ataccaaact gatcccacag
    18841 gtttttggca tctgcctgag atgcagcgct cttagctgag gttgccagcg acttcatctg
    18901 atttaaaaga tttttgatcg actcgattgt atctgtcgcc gtcttaattg tctgaacggc
    18961 ttcgctcatg ccatttttga atgttgcata gtcagatgca agctgatagt gggcgtcagc
    19021 gtcaaaatag gctacgggat catctatagc tgaattgact ttcttgcctg tggcaagtct
    19081 ttcctgagtg atacccatca attttgctgt gttctgaaga gataaaaggt tcgttgtcat
    19141 ctgtgctgtt aaagatattg ttgccatatg tttgttcctc cttctgagtt cttattttgg
    19201 ctttctcgcc atggaattct tgaagttctg cccttttctc ttgtccacct ccctcatggg
    19261 cattgtttga tccttcatga tttatttttc gcatgtttaa tttgaaactt tagaaacaat
    19321 gaattgaaaa atggagctta attaaagtct gttataatac ttctgaaata tgctcaaaag
    19381 agctggatac atgagtgtta gataatatga tgcagatatt catatcattc t
//
LOCUS       DAOOMC010000045        19373 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdus sp. isolate AMR_MDS_2685
            NODE_2847_length_19373_cov_6.927270, whole genome shotgun sequence.
ACCESSION   DAOOMC010000045 DAOOMC010000000
VERSION     DAOOMC010000045.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864760
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdus sp. (wastewater metagenome)
  ORGANISM  Syntrophorhabdus sp.
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae; Syntrophorhabdus.
REFERENCE   1  (bases 1 to 19373)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19373)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,729
            Genes (coding)                    :: 2,708
            CDSs (with protein)               :: 2,708
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 7 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19373
                     /organism="Syntrophorhabdus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2847_length_19373_cov_6.927270"
                     /isolate="AMR_MDS_2685"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2699756"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(179..373)
                     /locus_tag="PK125_06150"
     CDS             complement(179..373)
                     /locus_tag="PK125_06150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB37724.1"
                     /translation="MVLRVQYHNDKFDYVNDFALGRLITTNEIKKFYRPSEERWITVG
                     KDAVRGEGGYYTGPDRRQGN"
     gene            689..2368
                     /locus_tag="PK125_06155"
     CDS             689..2368
                     /locus_tag="PK125_06155"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HPB37725.1"
                     /translation="MKIKQIMMMSGMMLAQTSLVAFVLTGNSVTGINLYLILGLYGIS
                     LVFTIVWAFMSNKGMGNSVKQLLEISSEMGSNEGDLSKDFPVAVDGLGELSESYNHFL
                     RKLRAIIGDVRKMGVNIAVESVNVARRIKVSADSVLKQKELADIIFNYSDEATVAIGE
                     ISQNVQFIADSTDKNLRTAQGSLVELKDVTEKINNVSQKLNNFEITVKDLSQNSESIK
                     EVVSLIQDISDQTNLLALNAAIEAARAGEAGRGFAVVADEVRKLAEKARSATEEIAKN
                     VSEMLKHVNSTLSETEEINMYTQQTREVVERSSGNFERMVKDFENTNSQLSTIASAIE
                     ELSLTNGEIHGRVNEIHSLTMNVTKEMESSEKSSKDLGRITEDMQEMILRFKIGQGSY
                     EKVILTAKQHRDLVQQKLEELQNKGINIFDRNYKPVPNTNPQKYKTVYDNYFDKDIQA
                     LYDRALGDIEGASYAACVDINGYAATHNNKYSRPLTGNPETDAIWSRDKRIYNDEKGL
                     RAAKNVQQFLIQTYTTAAGETVNDLSMPIYVNGNHWGAMRVGLNPSTMLKD"
     gene            2629..3105
                     /locus_tag="PK125_06160"
     CDS             2629..3105
                     /locus_tag="PK125_06160"
                     /inference="COORDINATES: protein motif:HMM:NF024106.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative molybdenum carrier protein"
                     /protein_id="HPB37726.1"
                     /translation="MKIISGGQTGVDRAALDAAIGAQLDYGGSVPEGRMAEDGPIDVK
                     YVKMTEIPRGTYRDRTKKNVDDADATLIFTKGRPTDGTAYTIMYCRKSHKPFLVIDLV
                     DMGIAYPVQRVEEWLESVRPGVLNVAGPRESKSPGIYVQVIEILSLVFHRIAKVLD"
     gene            3257..3332
                     /locus_tag="PK125_06165"
     tRNA            3257..3332
                     /locus_tag="PK125_06165"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3293..3295,aa:Pro,seq:tgg)
     gene            complement(3388..4041)
                     /locus_tag="PK125_06170"
     CDS             complement(3388..4041)
                     /locus_tag="PK125_06170"
                     /inference="COORDINATES: protein motif:HMM:NF013077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016657 - oxidoreductase activity, acting
                     on NAD(P)H, nitrogenous group as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase"
                     /protein_id="HPB37727.1"
                     /translation="MDLIEAIDSRRSIRAFKPSPVSRELVEKILNLVVKAPSAINLQP
                     WEFIVVMGEEKERLSRKLIKSYREKQISCSPGNVKPLADTFSKRGLASVELMTPSLAK
                     LGHEFNQFINEGSCNFYGAPTAVILCLDNAFSKARLVDVGIGVGYFVLVAQSFGLGTC
                     TIGLINAYEDEIKEVLSIPENKDVVIGIALGYPDLDSPANEFKTPRDNLESFVRWFD"
     gene            complement(4142..4810)
                     /locus_tag="PK125_06175"
     CDS             complement(4142..4810)
                     /locus_tag="PK125_06175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB37728.1"
                     /translation="MNKKVKWLCSVIGLFIMISIILAVSFKEKILIGAGKFMAPESSL
                     TEGMADVVILEGTGTIGKGVVAKGIELLSSGQAKHMAIVLHSIGKNHWPFAFHEDYPS
                     SVKMELQKLGLKDSDFTIIVTPIRNPVTLTSAKGALQILAKDRIKEAILVSPGFHMRR
                     SFLVYQHLSLPLKIKIYPVACFDRYQLNDWWSHDYGPRDFLLELQKLALYMAKGYIPL
                     KISY"
     gene            complement(4862..5008)
                     /locus_tag="PK125_06180"
     CDS             complement(4862..5008)
                     /locus_tag="PK125_06180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB37729.1"
                     /translation="MEGFNSPVGGKDITGLYYGRVINGPFCAVTFSCNCGIQEHYLLS
                     CGSK"
     gene            5300..5653
                     /locus_tag="PK125_06185"
     CDS             5300..5653
                     /locus_tag="PK125_06185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB37730.1"
                     /translation="MIRAKLWFRCAAMHDPVTPMVAQPAQVGWEAKRRRVDLTIERSF
                     NGEELIKRMKGWVTTDPYRVIEVVRPYARMKVLDDRELVLELEREEDLEKLNKALQES
                     FGGEVDAELIVKRQR"
     gene            5775..7517
                     /gene="polX"
                     /locus_tag="PK125_06190"
     CDS             5775..7517
                     /gene="polX"
                     /locus_tag="PK125_06190"
                     /inference="COORDINATES: protein motif:HMM:NF006375.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase/3'-5' exonuclease PolX"
                     /protein_id="HPB37731.1"
                     /translation="MQKKPVSDILENIAMLLEIKGENPFKSRAYYNAAKALSGITNLE
                     ELVKEKRLREIKGIGEALSQKIEEYSKTGTMAYYEELTQVVPESLLELMQIPNLGPKK
                     IKVLYDELEITNIGELEYACKENRLIHLTGFGEKTQENVLKGIEFFKRHKGEFLFGDV
                     YQEAERIRQRLSTIVDPIWAEVCGSIRRRKEVVRDMDILVAAENHEKITSFFVSMPEV
                     DQVLVTGETKTSVRMKSGIEADLRVVTRQEFPYALVYFTGSKEHNVRLRGIAKKKGWK
                     LNEYGIFDGDNLVICKSEEEIYRALGLPYIPPELREDSGEIEAAEQDKLPSLVQHEDI
                     RGIFHVHTDFSDGVDSLERMVEAAQKFGFSYLGVSDHSKTAYYAGGLKHDAILRQWEM
                     IDTLNKKNSTFRIFKGIESDILSDGSLDYDDSILEGFDFVIASVHSGFTMKKDDMEER
                     ILKAMKNPYTTILGHPTGRLLLSRDGYQVDMMRIIDCAAQNHVILELNASPYRLDIDW
                     RYLKYAKDKGVMISINPDAHAVAGLEEVFFGVNIARKGWQESKDILNTRDVNDIKEIF
                     TKIRNAKRHQVNHS"
     gene            7486..8124
                     /gene="nth"
                     /locus_tag="PK125_06195"
     CDS             7486..8124
                     /gene="nth"
                     /locus_tag="PK125_06195"
                     /inference="COORDINATES: protein motif:HMM:TIGR01083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HPB37732.1"
                     /translation="MQKDIKSIIRNLKEMHGKPRCELIFSNPIELVVATVLSAQATDQ
                     KVNEVTRSLFTKYRTWKDYLNVPVGELEEDIRQTGFYRNKAKAIKNIALEIEGRFKGQ
                     VPRDVDSFVTVKGIGRKSANLIVGLAFGKPAIIVDTHVIRLAQRIGFTKNKNPDKIEL
                     DIKKIVPENLWTDFSLLITLHGRYLCKAKKPECEKCLIRESCDYFMGGKRDV"
     gene            8117..10021
                     /locus_tag="PK125_06200"
     CDS             8117..10021
                     /locus_tag="PK125_06200"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF004556.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HPB37733.1"
                     /translation="MFEKERLGEYEIKNLEELSRYAKGDIIKMTTLAGTGHPGGSMSS
                     LDIYLVVFFFAHLLDEPKDKVVVSHGHTSPGVYASLARLGYLPIHDVISFFRKAGSPF
                     EGHVVKGTPFIGWSTGNLGQGLSAGCGFALGAKLRGENSHVYVLMSDGEQQKGQVGEA
                     RRFAKKFGLSNITGIIDYNRIQISGNIEKVMPQNIIENYLSDGWDVIEVNGHDHNELY
                     KAMRRARGVDNPVCIVANTVIGKGVGFMEDTEKYHGNPLNQAEYRQAMEILDLEDDLE
                     FYRERRKGTCTWRPSEAITEEVIDSGSPFTYRDEDKVDNRTAFGKALKDLGDKNISKG
                     HLVCAFDCDLASSVKTADFAKAYPDYFFQSGIQEHNTATVAGALSTTGILTFFADFGV
                     FGIDETYNQHRLNDINKTNLKLITTHLGLDVGPDGKTHQCTDYIGLTSNLYHFKVIIP
                     CDPNQTDRAVRYAAQEKGNFLIGTGRNRWPVLSREDGKPLFAEGYSFEYGKMDSIREG
                     QDGAIITYGGMVARAIKIADALAKKGLSLKVVNMPCVREADALMLDELAQLPFVCTYE
                     DHNVHTGIAPFIAQQLLMRRYKGKVASFGAKDYGPSGETEEVMVAEGLDADSMIEALS
                     RMVKKKRGKR"
     gene            10131..11417
                     /locus_tag="PK125_06205"
     CDS             10131..11417
                     /locus_tag="PK125_06205"
                     /inference="COORDINATES: protein motif:HMM:NF013931.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB37734.1"
                     /translation="MEDIKGMYKKVVKDNFPDTIKIDMGGQVLLYRKRVWNIFDGDEK
                     CDVERGLRYGENPDQPSALYELINGNIILGDVRFFDFDGGLVSKVTEKDMLQVGKHPG
                     KINLTDVDNALNIMKYLDKKPACAILKHNNPCGAAYGASSAEAFEKAFWCDRIAAFGG
                     AVVFTRAMDVETAKAMVPYYFEVICAPDFEGGAIDILKKWKNLRLLQIKDIERLQSFK
                     SKRYVDFKSLMDGGLVLQESQPFRIKSKEDLRPAEAIYKGQTYKIKRTPTEQEYDDML
                     FGWYVESGVSSNSALFVKGEATVAIGTGEQDRVGVVEIAVFKAYTKFMDKEVFRKFGV
                     PYAVFKLEAQKGLRKMEDLIEIEKYTREQKAGLLGSVIVSDGFFPFRDGVDAAIKEGA
                     TGVIQPGGSERDFEVIEACNESNVSMVFTGQRLFKH"
     gene            11685..13406
                     /locus_tag="PK125_06210"
     CDS             11685..13406
                     /locus_tag="PK125_06210"
                     /inference="COORDINATES: protein motif:HMM:NF013225.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HPB37735.1"
                     /translation="MRPYFEKMQDWGKPVKENKANADEIKKIEAEIAELVQVAKNAGI
                     SQEVLNKRGEWTVHQRLEYIVEPGTWAPLHTLYDPMNEESGTTGVVDGLGRINGRWCV
                     IIGFDNKVMAGAWIAGQPHNILRVTDIAKRLHCPLVWLVNCSGVKLPEQEKMYADRRG
                     SGTTFFRHAELNKLGIPVLAAIYGTNPAGGGYQGISPTLLLAHKDCNIAVGGAGIVSG
                     MSPKGFFDEETAEQLIEATRKFKAVPPGRVEIHYDHTGFFKQVFQTEEAILDAVKEWV
                     TYLPAYNHYKFFRVAEPAEPKFPAEDLYSIVAFNQKMTYDVEQVMARLSDNSEHMEFR
                     PGYGPELYTGLIKVDGFLMGIVANRQGMMPRGYPEYAPYPGIGGKFYRQGLIKVNEFV
                     TLCGRDRIPMIWIQDTSGIDVGDIAEKAELLGLGQALIYSIEQSDLPMMTVVLRKGTA
                     AAHYIMAGPQANNNNCFTLGTATTEIYVMHGETAAAASFARRLVKEKDAGKPLGKVIE
                     QMNKLVKSYYDNSRPKYCAKAGLVDEVVPMKDLRKYFVAFANCCYQNPASITPQHQMI
                     LCRIIKG"
     gene            13574..14743
                     /gene="bioF"
                     /locus_tag="PK125_06215"
     CDS             13574..14743
                     /gene="bioF"
                     /locus_tag="PK125_06215"
                     /EC_number="2.3.1.47"
                     /inference="COORDINATES: protein motif:HMM:TIGR00858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008710 - 8-amino-7-oxononanoate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HPB37736.1"
                     /translation="MRDRLSSTLSKIREKGNYRTIKYLEPLSSTRIMYNGREYLNLCS
                     NSYLSLHTHQKVVEAAKQATDRYGAGICSSRSVSGSIDLYGILEEEIAAYKGYKKGII
                     FSNGYMANIGIISTLTREGDTIFSDELNHSSLIDSMRLSRAKKIIYKHKDIDDLERKI
                     KKENTTGRRFIVTETVFSMDGDIAPLADLHKLKERYEASIIVDDAHGTGVFGRQGIGV
                     EELHSLCGAMDVHMGTFGKSLGSFGAFVLSDEVIIDFLINRARTFMYTTALPPAALGA
                     ARAALGLVQKSISFKDELWSNIEYLRDGLKEAGFNLQESFGPIIPIIVGEDKAAVEMQ
                     EELLEQGLFLQAIRPPTVPGGTSRLRFTVVRDLTREDMNYTIEALRAVGKKHGLV"
     gene            14854..16206
                     /gene="bioA"
                     /locus_tag="PK125_06220"
     CDS             14854..16206
                     /gene="bioA"
                     /locus_tag="PK125_06220"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: protein motif:HMM:TIGR00508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004015 -
                     adenosylmethionine-8-amino-7-oxononanoate transaminase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HPB37737.1"
                     /translation="MYSKKQLMDWDKEYIWHPFTQMKDYMDMNPLVIQKGEGCYLVDT
                     SGNRYIDGVSSLWVLVHGHGKKELVDAIQKQSKTLCHSTLLGLANVPSVILAKKLIEI
                     VPKGLSKVFYSDNGSTSVEIALKMAYQYWQQKGEKRRKRFIAFTNAYHGDTIGAVSVG
                     GIDLFHQVYGPLLFKTFKAPSPYCYRCPMKLRQETCGMACVDAFEKVVKKHHEETCAV
                     IIEPLVQGAAGMILQPPGWLNAIWKIAKEYGLLFIADEVATGFGRTGNLFACEEERIS
                     PDFMCVAKGITGGYLPLAATLSTEEVFNGFLGQFDEFKTFFHGHTYTGNPIACAVAVE
                     SINLFKKEGLLQKLQQKTAILKNSLDRFKELTHVGEVRQKGFMVGIELVKSRKTKRSY
                     APGDKIGQKVIWEARKRSVIIRPLGDIIVLMPPLAIDRPTLEELVDVTYESIKAVTGS
                     "
     gene            16242..16547
                     /locus_tag="PK125_06225"
     CDS             16242..16547
                     /locus_tag="PK125_06225"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative quinol monooxygenase"
                     /protein_id="HPB37738.1"
                     /translation="MIHVIASVKVKPGKRDQLLDLFRENLPRVREEKGCIRYFPAVDV
                     ATGLPPQSLDEDIITVIETWESLEALKDHLAAPHMAAFFEKEKPLVSGSVIKILQEV"
     gene            complement(16764..17348)
                     /locus_tag="PK125_06230"
     CDS             complement(16764..17348)
                     /locus_tag="PK125_06230"
                     /inference="COORDINATES: protein motif:HMM:NF013382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HPB37739.1"
                     /translation="MSYSKLPGSGKSSYHLVDHELVFHELGLTKSKIFLDLGCGIGDY
                     VIAAAEIIGPGGIAYGIDGWTEGIDILKKRAQDRRLTNVQTFVASVSDHLPLPDQTVD
                     ICLIAAALHDFIRHGGIEVPLKETARVLKPAGILGVVEFKKIDGPPGPPVLIRLSPEE
                     TGTLIASFGFQQIKTLGVGPYTYLIVASKMNQFI"
     gene            complement(17377..18471)
                     /locus_tag="PK125_06235"
     CDS             complement(17377..18471)
                     /locus_tag="PK125_06235"
                     /inference="COORDINATES: protein motif:HMM:NF018805.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin-like domain-containing protein"
                     /protein_id="HPB37740.1"
                     /translation="MLLIIIAFVFPIRTAMAVEKDWKQAIGPWQWSFPRDHGAHPEYR
                     TEWWYFTGNLRDREGNQYGYQLTFFRQGLTLVAPWPDNPWSIRDVYLAHFAIVDRTSG
                     KFWYYDQASRSGPGLAGAKLGNLDVWTLNWSAKMIGNTIHLKAHMDKMALDLEISPRK
                     PRVFHGDNGLSTKGPNPGQASYYYSLTDLATKGWIQTELSKNYFAVTGISWFDQEFGS
                     NVLGSDQVGWDWFGIHLSDGRDIMLFQLRKKDGTIEACSSGTIIEKDGSSRHLRQSEI
                     SLNVKEHWKSPKSGGTYPARWGIKIPSTGIDITLTPIVASQELVTEASTGVTYWEGAV
                     TGSGISKGISVTVEGYAELTGYAGSLDRVF"
     gene            complement(18630..>19373)
                     /locus_tag="PK125_06240"
     CDS             complement(18630..>19373)
                     /locus_tag="PK125_06240"
                     /inference="COORDINATES: protein motif:HMM:NF014717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HPB37741.1"
                     /translation="NGGVIISESFARRFKTKEGDRVTLDGIEGPVALSVGAIFYDYTT
                     EHGLIMMDRSTYIKIFGDTTINSLGIFIDPGNPQRAELLGEIRRKAQERNLPVLTSKQ
                     LERNILALFDSTFAVTRSMRFLAIIVAFFGITGALLTLFLERRKDFGIYRALGFSTSQ
                     IGIMTVLEGLGMGLVSFLLSTVGGTALALILIKVINLRSFNWTVFYYPEWQPYVVTGI
                     VAICASLGAALHPLWKVCRTYPQMQIREE"
BASE COUNT         5728 a         3929 c         4737 g         4979 t
ORIGIN      
        1 atatccaaaa aattgatcat atcgcctgcg ttttcagctt ttagaaagat tcaggttaga
       61 atggtggaga agaggagatt ttgagacagg gttgaagggg gtcaaccctg tcattgaggg
      121 tgtatcataa agggggttat gagaagttac tgaattaaaa aaaaacgtat caccggggct
      181 aattaccctg ccttctgtct ggaccagtat aatagccacc ttcacccctc acagcgtctt
      241 tgcccacagt gatccacctt tcttctgagg ggcgatagaa ttttttaatc tcatttgtgg
      301 tgataagcct tccaagggca aaatcattca catagtcgaa cttatcattg tgatattgaa
      361 ctcgtaagac cataaatgcc tccctggtgt gtttgatgaa agataaatat gcaaggcctg
      421 tgccggatta aaaggcagaa tgaggcgtaa gccatgaaaa gtaaaaagca agaggccaaa
      481 gtggaactgg cggggcaaat tgctcatcag gcttgttgaa caggctgagt ggtttatcga
      541 attaacccaa tcaacaaccc gaaagctcaa tcaatgtgga gaatttaaaa aggaatgacc
      601 gttaaaatat tgacaaatta ttaattttga ccagaattgt gacgataatc acattattat
      661 gtagaaacac taaggaggtg tttttgctat gaagattaaa cagataatga tgatgtctgg
      721 aatgatgttg gcccagacct cactggttgc ctttgtgctt acaggtaata gtgttaccgg
      781 tatcaacctg tacttgattc tgggactcta tggcatatca ctggtgttta ctattgtatg
      841 ggcattcatg tcaaacaaag gtatgggcaa ctcagtaaaa cagttactcg aaatatcgtc
      901 agagatggga agtaatgaag gtgatctttc aaaagatttt cctgttgccg ttgatggact
      961 cggtgaatta tcagaatctt acaatcattt tttaagaaaa ttacgggcta ttattggtga
     1021 tgtgcggaag atgggtgtta atattgcggt agagtcagta aatgtggccc gaaggatcaa
     1081 ggtatcagca gacagtgtat taaagcagaa agagcttgca gacattattt tcaattacag
     1141 cgatgaagct actgttgcca taggcgaaat ctcacagaat gtgcagttta ttgctgactc
     1201 gacagataaa aatctgagga cagcccaggg atccctcgta gaattaaagg atgttaccga
     1261 aaagataaac aatgtgagtc agaaactgaa caattttgaa attactgtaa aagatctgag
     1321 ccagaattca gaaagtatta aggaagtggt ctccctgatt caggatattt ctgatcagac
     1381 gaaccttctc gcgttgaatg ccgcaattga agctgcaaga gcaggtgaag cagggcgtgg
     1441 ttttgctgtg gttgcggacg aagtgaggaa actggcagag aaggccaggt ctgcaacaga
     1501 agaaattgcc aaaaacgtaa gcgaaatgtt gaaacatgtc aattcgacac tgagtgagac
     1561 agaggagatt aacatgtata ctcagcaaac gagggaagtt gttgagagaa gttcaggaaa
     1621 cttcgaaaga atggtcaaag actttgagaa tacaaattcc cagctatcta ccatagcgtc
     1681 tgccattgaa gagctttctc ttaccaacgg tgagatacat ggcagagtga atgaaataca
     1741 tagcctcaca atgaatgtta caaaggaaat ggagtcctca gagaaatctt caaaagatct
     1801 gggcaggatt accgaggata tgcaggagat gattctccgg tttaaaatag ggcagggcag
     1861 ttacgagaag gtgattctta ctgcaaagca acacagagat cttgtccagc aaaaacttga
     1921 agagttgcaa aacaagggca taaatatctt tgacagaaat tataagcctg tcccaaacac
     1981 caacccgcag aagtataaaa cagtatatga caattatttt gataaggata tccaggccct
     2041 ttatgatcga gcgctggggg atattgaagg ggcatcctat gcggcgtgtg tagacatcaa
     2101 cggatatgca gcaacccaca acaataagta ttcaaggcca ttaacaggca atcctgagac
     2161 cgatgcaata tggagcaggg ataaacgcat ttataacgat gaaaaaggcc tgagggcggc
     2221 aaagaatgtt cagcagtttc tgattcagac gtataccaca gcagcagggg aaacggtcaa
     2281 tgacctgtcc atgccgattt acgtcaatgg gaatcactgg ggtgcaatga gggtagggct
     2341 taatccttca acaatgctaa aggattgaag agataaaggt tcgtcatgaa gacagaaaaa
     2401 actaggagct gaccagcaat cacgttgagg agccgacacc actgaccctg taatccgtat
     2461 cttttcatca cagattctat acaggcgaga gaaagccttg atgcatgaaa aaatttagta
     2521 taatatgacc tcgaaactga caaactgttt gctgtgcact gtctgaaaat cgcaaaatcg
     2581 agcgcaggaa gagaacggag tccacaatct caaaaaaaag gtaggcccat gaaaataatc
     2641 tcaggagggc agacaggtgt tgacagggca gccctcgacg cagctattgg cgcacaattg
     2701 gattatggcg gttcagtgcc tgagggcaga atggctgagg acggcccaat tgatgtgaag
     2761 tacgtaaaga tgacagaaat acctcgcggt acatacaggg atagaactaa aaagaatgtt
     2821 gatgatgctg atgcaacgct gattttcaca aagggaagac ccactgatgg tacagcctat
     2881 acaataatgt attgtcgaaa atcacacaag cccttcctgg ttattgatct ggttgatatg
     2941 ggtattgcat accctgttca aagggtggag gaatggcttg agtctgtgcg tcccggggtg
     3001 ctcaatgttg caggcccaag agaatcaaaa tcaccgggaa tctatgttca ggtcattgaa
     3061 atcctgtcgt tggtttttca caggattgct aaagttctgg actaaacatt gaggcgatgt
     3121 ttttttcata aacaccgaag gtttgcaggt ctttgggaaa acctttattg attgctcaga
     3181 gcgctccatt ggtttgcccg tgggaatctt tcgttaaaat tgcaaaatat gtaatcctgt
     3241 ggtagattaa ctgcatcggg gcgtggcgca gcatggttta gcgcgcttgc cttgggagca
     3301 agaggtcgct ggttcaaatc cagtcgcccc gattttaaat cgaattaaaa ttctaatatc
     3361 gaaataactg aaaaaataga ccctatccta atcgaaccat cgcacaaatg attcgaggtt
     3421 atcccgtggt gttttgaatt cattggccgg gctgtcaagg tcagggtagc ccaatgcaat
     3481 accaatgacc acatccttat tctccgggat gctcaatacc tcttttattt catcttcata
     3541 agcgttgata aggccgatcg tgcatgttcc cagcccgaag ctttgcgcaa cgagaacaaa
     3601 atatccaaca ccaatcccca catccacaag acgagccttt gagaaagcat tatcaaggca
     3661 gagtattaca gcagtcggag ccccgtaaaa gttacaactc ccctcgttaa tgaactggtt
     3721 aaattcatgg cccagcttgg caagggatgg agtcatgagt tctacagatg caagccccct
     3781 tttgctaaaa gtgtctgcca ggggcttgac attgcctggg ctgcaggata tctgtttttc
     3841 ccggtaggac ttgatgagtt tgcggctcaa cctttctttt tcctcgccca tgacaacgat
     3901 aaattcccat ggttggaggt tgattgcaga gggtgccttg acaacaagat tcaaaatctt
     3961 ttcaacaagt tctctggaaa caggacttgg tttaaaggcc cgaatgctcc ggcgactatc
     4021 aattgcttcg ataagatcca tacgtacctg acctcccttt cacttcaaac ttctcttgaa
     4081 ggataaccgc aattatcagt gatgtcaaga cagagcctta acagagctgc gcagcttaca
     4141 tttaataaga tatcttcaac gggatatacc cctttgccat gtacaatgcg agcttttgaa
     4201 gctctaacaa aaaatcacga gggccataat catggctcca ccagtcatta agctgatacc
     4261 tgtcaaaaca ggcaacaggg tatattttga ttttcaatgg aagggaaaga tgctgatata
     4321 cgagaaaact tctccgcatg tggaatcctg gtgacacgag gatggcttct ttgatcctgt
     4381 ctttggcgag aatctgtaac gctccttttg ctgatgtcaa cgtgactgga ttccgtatag
     4441 gggttacgat tatggtgaaa tccgaatcct tcaagccgag tttctgtaat tccatcttta
     4501 cggaagaggg atagtcttca tgaaaggcga agggccaatg attttttcca atactgtgca
     4561 ggactatggc catatgtttt gcctggcccg atgacaataa ctctatccct ttagcaacca
     4621 cgcccttccc gattgttcct gttccttcca gaataaccac atctgccata ccttctgtga
     4681 gactggattc gggagccatg aacttacctg ccccaatcag gattttctct ttgaatgaga
     4741 ctgcgaggat tatgctgatc attataaaaa gaccgatcac actgcaaagc cattttactt
     4801 ttttgttcat gatctgttct gcctcaattc aggactcgtt gttatgtcat atattatctg
     4861 atcattttga tccacaagaa agaagataat gttcttgaat gccacaatta caggaaaaag
     4921 tgacagcaca gaatggacca ttgatgaccc ttccataata gaggcctgta atgtcctttc
     4981 caccaacagg gctgttaaag ccttccattc aggctcatgg cattccctct atagttaccc
     5041 atgggagagg taagccgcgt aaggggacca tgcttttaag ggtaggtgaa ttgacttatg
     5101 ctttcacacc ggtgtgtata tttttgtgcc acggggttgc ctgcaggaat cttcattttt
     5161 tcagtgaggg tcagtttttt ttactgtgca gggcatcagt tttagggaag atgcaattaa
     5221 ccttggcttg acatcagaat agtaaacctt tattgttaaa ctacaggtga atattagtta
     5281 ttctggaagg agatagagaa tgatacgagc aaaattatgg tttcgttgcg ctgcaatgca
     5341 tgatcctgtt accccaatgg ttgctcagcc tgcccaggtt ggctgggagg caaagaggag
     5401 aagggtcgac ctcaccatag agcgttcatt caatggggaa gaacttatca aaaggatgaa
     5461 aggttgggta acaacagacc cgtacagggt cattgaagtg gtcagaccct atgcgaggat
     5521 gaaggtgctt gacgacagag agcttgtcct ggagcttgaa cgcgaagaag accttgaaaa
     5581 attgaacaag gcactgcagg aatcttttgg cggagaagtc gacgcagagc taattgttaa
     5641 gcggcaaagg tagaaaaaag taacagtcaa aagtgaagac taaagagccc gggaggtatg
     5701 attaaaactg caacgcagga ggggttgctg ggtaactgat caatactgtt tcaggactat
     5761 gaagaattag tggtatgcag aaaaaaccag tcagcgacat tcttgaaaac attgccatgc
     5821 ttcttgaaat aaaaggggaa aacccattta aatcgagggc atactacaat gctgccaagg
     5881 ccctatccgg tataaccaac cttgaagaac tggtaaagga aaaacgattg cgagagatca
     5941 aaggcatagg cgaggccctc tcacaaaaaa tagaggaata tagcaaaaca gggactatgg
     6001 catattacga ggaactgacg caggtagttc ctgagtcgct tctcgaactc atgcaaattc
     6061 ctaatcttgg tccaaaaaag atcaaggttc tctatgatga gcttgaaatc acaaatattg
     6121 gtgagcttga gtatgcgtgc aaggaaaaca ggctcattca cctgaccggt tttggtgaaa
     6181 aaacccagga aaatgtactc aaaggcattg aattctttaa gcgacacaag ggcgagttcc
     6241 tgttcggcga tgtttatcag gaagcagaga ggatcaggca gcgcttatcg acaattgttg
     6301 accctatatg ggctgaggta tgtggaagca ttcggcggcg aaaagaggtt gtccgagaca
     6361 tggatatcct tgttgctgcg gaaaaccatg agaagattac gtcatttttt gtatccatgc
     6421 ctgaagtgga tcaagtgctt gttaccggtg aaactaaaac ctcggtgaga atgaaatcag
     6481 gtatcgaagc ggatcttcgg gtggtgacca ggcaggaatt tccctatgcc cttgtatatt
     6541 tcacaggaag taaggaacac aatgtaaggt taagagggat tgcaaaaaag aaagggtgga
     6601 aactgaatga atacgggata tttgacgggg ataacctggt aatatgcaaa agcgaagagg
     6661 agatttatcg tgccctgggt cttccctata tccctccaga actgagggag gattcaggag
     6721 agattgaagc ggctgaacag gataagcttc cctcactggt acagcacgag gacattcgcg
     6781 gcatctttca cgttcatact gatttcagtg atggtgtcga ttcccttgaa aggatggtgg
     6841 aagcagctca aaaatttggt ttttcgtacc tcggtgtttc tgatcacagt aagactgcat
     6901 actatgcagg aggattaaaa cacgatgcca tccttagaca gtgggagatg attgacacat
     6961 taaataaaaa aaattcgacg tttcgcatct tcaaaggcat cgagagcgac attctatctg
     7021 atggaagcct tgactatgat gattctatcc ttgaaggttt tgattttgta atagcctctg
     7081 tccattccgg tttcaccatg aaaaaagacg atatggagga aaggattttg aaggctatga
     7141 aaaacccata cactactatt ctgggccacc cgaccgggcg cctgctcctt tcccgggatg
     7201 gttaccaggt tgatatgatg agaataattg attgtgccgc gcaaaaccat gtgatcctcg
     7261 aactgaatgc aagcccatac cgactggaca ttgactggag atatctcaag tatgcaaagg
     7321 ataagggtgt catgatttcc atcaaccctg atgcccatgc tgttgcaggt cttgaagagg
     7381 ttttctttgg ggttaatatt gccagaaagg ggtggcagga aagtaaagat atattgaata
     7441 caagagatgt aaatgatata aaagaaatat tcacgaaaat ccgcaatgca aaaagacatc
     7501 aagtcaatca ttcgtaatct taaggagatg cacggcaagc cccggtgtga actcattttt
     7561 tcgaatccca tcgaacttgt tgttgctact gtcctttcag cccaggccac tgaccagaag
     7621 gtgaacgaag ttaccagatc gctttttact aaataccgga cgtggaaaga ttatttgaat
     7681 gtaccagtcg gggagttgga agaagacatc cggcaaactg gtttttaccg gaataaggca
     7741 aaagcaatca aaaatattgc cctggaaatc gagggacgct ttaagggaca ggttccccgg
     7801 gacgtggatt cctttgttac tgtgaagggt attggcagga agtcagcaaa tttaattgtg
     7861 gggcttgctt ttggaaaacc tgccatcatc gtggacaccc atgtcataag attggcccag
     7921 cggataggat ttacaaaaaa taaaaaccct gacaagattg aattggatat caaaaaaatt
     7981 gtacctgaga atttgtggac agacttttct ctcctcataa cgctccacgg gcgctatctt
     8041 tgtaaagcaa aaaagcccga gtgcgagaaa tgtctgatac gtgagagttg tgattatttc
     8101 atgggaggga agagagatgt ttgaaaaaga acggctgggc gagtatgaga taaagaatct
     8161 tgaagaacta tcgcggtatg cgaaaggcga tattattaaa atgactactc tcgcagggac
     8221 agggcatccg ggaggctcta tgtcatctct tgacatatac ctggtagttt ttttctttgc
     8281 ccatctcctc gatgagccaa aagataaggt tgttgtcagc catggccaca cttcgcccgg
     8341 tgtttatgcg agtctggcac gccttggata cctgccaatc cacgatgtta tatcgttttt
     8401 cagaaaggca ggcagtccct ttgaggggca tgttgtaaaa ggaacgccgt ttattggctg
     8461 gtcaacaggc aaccttggcc agggcctttc tgcagggtgt gggtttgccc ttggagcgaa
     8521 gcttcggggt gaaaacagcc atgtgtatgt tcttatgagc gatggagaac aacagaaggg
     8581 ccaggtggga gaagcgaggc gttttgcaaa aaaattcgga ttgtcaaaca ttacaggaat
     8641 aattgactac aaccggatcc aaatcagcgg aaatatagag aaagtcatgc cccagaatat
     8701 tatagagaac tatctttctg acggatggga tgtgattgaa gtgaacggtc atgaccacaa
     8761 tgaactttac aaagccatga gaagggcgcg cggagttgat aatcctgtat gtatcgttgc
     8821 aaatacggtc attggcaagg gtgtgggttt tatggaagac accgagaagt accacggaaa
     8881 ccctctgaat caggcggaat acaggcaggc aatggaaatc ctcgaccttg aggacgacct
     8941 tgaattttac agggaaaggc gaaaagggac atgtacatgg cgtccctctg aagccataac
     9001 agaagaggtt attgatagcg gttcaccttt cacataccgc gatgaagata aggttgataa
     9061 tcgaaccgca ttcgggaaag cactgaagga tcttggagat aagaatataa gcaagggcca
     9121 cctggtgtgc gccttcgact gcgaccttgc aagttctgtg aaaacggccg attttgcaaa
     9181 ggcctatcct gattatttct ttcaaagcgg aattcaggaa cacaatacag caaccgttgc
     9241 cggtgcctta tccaccaccg gtatcctgac cttttttgct gattttggtg tgtttggaat
     9301 cgatgaaacc tataatcaac acaggctgaa cgatatcaac aagacaaatc tcaagcttat
     9361 caccacacat ctgggactcg atgtgggacc tgatggaaag acgcaccagt gtactgatta
     9421 tattggactg acgagcaatc tttaccattt caaggttatc attccctgcg atcccaatca
     9481 gacagatcgc gctgtcaggt acgctgcgca ggaaaaaggt aattttctta ttgggaccgg
     9541 aagaaataga tggccagtat taagccggga ggatggcaag ccattattcg cagaaggcta
     9601 tagcttcgaa tatggaaaga tggatagtat ccgagaaggg caggatggtg ctatcattac
     9661 ctatggtggt atggttgcca gggccataaa aattgctgat gcgcttgcga agaaggggct
     9721 atcgcttaag gtcgtaaata tgccatgtgt ccgtgaagcc gatgcgctga tgctcgatga
     9781 acttgcacaa cttccattcg tgtgtacata tgaggatcac aatgtacaca ccggtattgc
     9841 cccatttatt gcgcaacaac tcttaatgcg ccgctacaaa ggaaaggtgg catcattcgg
     9901 tgccaaggac tatggaccat caggtgaaac tgaagaggtt atggttgcgg aaggtctcga
     9961 tgctgactcc atgatagaag ccttatcacg gatggtaaag aagaaaagag gtaagaggta
    10021 aaagcgagtg cagaaaagcc gtaacgagta atcagtgacg cgtaatgggt ttaaaaaaaa
    10081 tcgatcaccg atcacagatt acgcgttacg gattataagg gggataaata atggaagaca
    10141 tcaaagggat gtacaaaaag gttgtaaagg ataattttcc tgacactatc aagattgaca
    10201 tggggggaca ggttcttcta tacaggaagc gggtttggaa tatttttgac ggagatgaaa
    10261 aatgtgatgt ggaacgggga cttcgttatg gtgaaaatcc agatcaacca tctgccctgt
    10321 acgaactcat taacggaaac ataattttag gagatgttcg tttttttgac tttgatggtg
    10381 gactggtaag caaggtaaca gagaaggata tgctccaggt aggcaaacat ccggggaaga
    10441 taaacctcac cgatgttgac aacgccctga acatcatgaa atatcttgat aagaaaccgg
    10501 catgtgccat actgaagcac aataacccgt gcggtgcagc ttacggggcc tcttctgccg
    10561 aggcctttga aaaagcattc tggtgtgaca ggatcgctgc attcggcgga gcggttgttt
    10621 tcaccagggc aatggatgtg gaaacagcaa aggcaatggt gccgtattat ttcgaagtga
    10681 tatgtgcacc tgactttgag ggaggagcca tcgacatatt gaagaagtgg aagaacctca
    10741 ggctgcttca gataaaagat attgaacggc ttcagagttt taaaagtaaa cgatacgttg
    10801 actttaaatc cctcatggat ggtgggctgg tccttcagga gtcgcagcct ttcagaataa
    10861 agagcaagga ggatctcaga ccggcggagg cgatctataa gggccagacc tataagatca
    10921 aaaggacgcc aacggaacag gaatatgacg atatgctttt cggctggtat gttgaatcag
    10981 gcgtcagttc caactccgcg ctttttgtaa aaggtgaggc taccgtagca attggaacag
    11041 gagaacagga cagggtaggt gttgtggaga ttgctgtgtt taaggcatat acgaaattta
    11101 tggataagga agttttcagg aaatttggtg ttccctatgc agtctttaag cttgaagcac
    11161 aaaaaggctt aagaaagatg gaagatctca tagaaataga gaaatataca cgggaacaga
    11221 aggccggact ccttggttcg gtaattgttt cagacgggtt ttttccattc agggatggtg
    11281 tggatgcagc tatcaaggaa ggagccacag gtgttatcca gccaggtgga tctgagcggg
    11341 attttgaagt gattgaggcc tgcaatgagt cgaatgtgtc catggtattt acagggcaga
    11401 gactgtttaa acattaacga ctctcagcga ttggcaataa gatcagagca gttaaaccgt
    11461 aaaatgatgg gtagaagaca tttgacaaaa cagactttga ccgagaacgc cagtaatgaa
    11521 aaaaagtaca gggtaaatgc agtgcttctg atgaatggaa aaaacataga aaaacatcaa
    11581 atatcgttac gttttctgtt gacaaaacgc tccctaaaaa atataattgt gaaaatatga
    11641 acaaatagct atgaatcaaa ataaaatgcc accggaggta agggatgaga ccgtattttg
    11701 aaaaaatgca ggattggggt aaaccagtaa aagaaaataa ggcgaatgct gatgagatca
    11761 aaaaaatcga agcagaaatt gcagagcttg ttcaggtagc aaaaaatgct ggcatttccc
    11821 aggaagtgtt gaataagaga ggtgaatgga cagttcatca gagactcgaa tacattgtgg
    11881 agccgggtac atgggctcct cttcatacgc tgtatgatcc catgaatgaa gagtcaggaa
    11941 caacaggcgt tgtggatgga ttgggcagga ttaacgggag atggtgcgta atcataggtt
    12001 tcgacaacaa agttatggca ggcgcatgga ttgcaggcca gccccataac attctccggg
    12061 ttactgacat agctaaaaga cttcactgcc cgctggtatg gcttgtaaac tgcagcgggg
    12121 taaaacttcc agaacaggaa aagatgtatg cagacaggag aggaagcggg accacattct
    12181 tcaggcacgc tgagctgaat aagctcggta tacctgtcct tgcagccatt tacggaacaa
    12241 acccggctgg cggcggttac cagggtattt ctccgactct tcttctcgca cacaaagact
    12301 gtaacattgc agtcggtggt gccggtatcg tgagtggtat gtcaccaaaa ggcttttttg
    12361 atgaagaaac agcagaacag cttatcgaag caacgagaaa attcaaagca gtgcctcctg
    12421 gcagagttga aatacattat gaccatacag ggttcttcaa acaggtattc cagacagaag
    12481 aagcaatcct cgatgcagtg aaagagtggg tcacctatct ccctgcttat aaccactata
    12541 agttcttcag ggtagctgag ccggcagaac cgaaattccc ggctgaagac ctttacagca
    12601 tcgtggcttt taatcagaag atgacctatg atgtggaaca ggtcatggca aggctttcag
    12661 acaacagcga gcacatggag ttcaggcctg gttatggtcc ggagctttat acaggcctca
    12721 tcaaggttga tggcttcctc atgggaatcg ttgcaaaccg acagggtatg atgccgagag
    12781 gttatccgga atacgcacca tacccgggca ttgggggcaa gttttaccgc cagggcctca
    12841 ttaaagtgaa tgagttcgta accctttgcg gcagagatag aatccctatg atctggatcc
    12901 aggatacctc aggtattgat gttggtgaca ttgctgagaa ggcagagctt ctcgggcttg
    12961 gacaggctct catatactcc attgagcaga gcgatcttcc catgatgact gttgtattga
    13021 ggaaaggaac cgctgctgca cactacatca tggccggacc gcaggcaaat aacaacaact
    13081 gttttactct cggcacagca acaactgaga tctacgtcat gcacggagaa acagctgctg
    13141 cagcaagctt tgcaagaagg ctcgtaaaag aaaaagacgc cggtaaacct ctcggaaaag
    13201 ttatcgagca gatgaacaag ctcgtaaaat cctactacga taattcaagg ccgaaatact
    13261 gtgcgaaggc cggtcttgta gatgaggttg tgccaatgaa ggatttgaga aagtattttg
    13321 ttgcattcgc taactgctgc taccagaacc cggcttctat tacaccacag caccagatga
    13381 tcctttgcag aatcattaaa ggctaataac cgtacgctga ttcaacaggg tgggttgatt
    13441 gacccaccct gtttttttaa gaaacaagtg ttaaccatta agatgaatga tgttgactgc
    13501 tgtgcagtaa atagtgtaat tttcaagtac aggctttgta tttattcttc ttaaggggtc
    13561 ccaaccacaa aaaatgagag acaggttatc ctccacactg agtaagatta gggagaaagg
    13621 caactatcga acgataaaat accttgagcc tctgtcttca acacgaatca tgtacaatgg
    13681 gagagaatac ctcaatcttt gctccaacag ctacctttcc ctgcacacac atcaaaaggt
    13741 tgtagaggct gcgaaacagg caacggacag gtatggtgct ggaatatgtt cttccagaag
    13801 cgtatcaggc agcatcgacc tttatggaat ccttgaggaa gagattgccg catataaggg
    13861 ttacaaaaag ggaatcatat tttcaaatgg ttacatggca aacataggca ttatctcaac
    13921 cctgacccgt gaaggcgata ctatttttag cgatgagttg aaccattcaa gccttatcga
    13981 cagcatgaga ctgtcgaggg ctaagaagat catttacaag cacaaggata ttgatgatct
    14041 ggaaaggaag ataaagaaag aaaatacaac aggaagacgg ttcattgtca ctgaaaccgt
    14101 atttagcatg gatggagaca ttgcgcctct tgctgatctt cacaagctga aagaacgtta
    14161 cgaggcctct attattgttg atgacgccca tggaaccgga gtcttcggca ggcagggaat
    14221 aggtgttgaa gaattacatt cactgtgtgg tgcaatggat gtgcacatgg gtacctttgg
    14281 taagtctctg ggatcctttg gggcctttgt gctttctgac gaagtgatta ttgattttct
    14341 catcaataga gccagaacat tcatgtatac cacagcattg ccacctgcag cccttggcgc
    14401 ggcccgcgct gcacttggtc ttgttcaaaa aagcatttct tttaaagatg aactttggag
    14461 taatatagaa tacctcaggg atgggctcaa agaggctggc tttaatcttc aggagagttt
    14521 tggccccata atcccaatta tcgtgggtga agataaggct gctgttgaga tgcaggaaga
    14581 gctcctggaa cagggtcttt ttcttcaggc aataagaccg cctacagttc cgggagggac
    14641 ctcgaggctt cgatttacag tcgtgaggga tctgaccagg gaggatatga attataccat
    14701 tgaagcttta agggctgttg gcaaaaaaca cgggcttgtt taatggggaa tgtgtggtat
    14761 gtgttcccag ttcaccgtaa agcaacatgg tcatcttttt atggaatata aacaggtttt
    14821 atataatggc gctatgtata tgtgggagga gaaatgtaca gtaagaaaca gctaatggac
    14881 tgggataaag aatacatctg gcaccctttt acacagatga aagattacat ggacatgaat
    14941 ccccttgtaa ttcagaaagg agagggatgt tatcttgttg atacgtcagg caatagatac
    15001 attgatggtg tatcatcgtt gtgggttctt gtccacggcc atggaaaaaa agagctggta
    15061 gatgccatac aaaagcagtc aaaaaccctc tgtcattcta ctttgcttgg ccttgcaaac
    15121 gtaccgtcag ttattctggc aaagaaactc attgaaatag taccgaaagg cctttcaaaa
    15181 gtcttctatt ctgacaatgg ttcaacatca gtggaaattg cactgaagat ggcctatcag
    15241 tactggcagc agaagggaga gaaaaggagg aagcggttta ttgcgtttac gaatgcatac
    15301 cacggcgaca ccataggcgc tgtcagtgtt ggaggtatcg atcttttcca ccaagtgtat
    15361 gggccgctgc tctttaaaac atttaaagcg ccttcaccct attgttaccg gtgtcccatg
    15421 aaactcaggc aggagacctg tggaatggca tgtgttgacg cctttgagaa ggttgttaag
    15481 aaacatcatg aagaaacctg cgccgtaatc attgaacctc ttgttcaggg ggctgctgga
    15541 atgattcttc aacctccagg atggcttaat gctatctgga agatagcaaa ggagtatgga
    15601 ctgttgttta tagccgacga ggttgcaacc ggattcggtc gaaccggaaa cctgtttgcc
    15661 tgtgaagagg agagaatatc ccctgatttc atgtgcgttg cgaaggggat taccggggga
    15721 tacctgcctt tggctgcaac actttcaact gaagaagttt ttaacggttt cctcgggcag
    15781 tttgatgaat tcaaaacatt ctttcatggc cacacctata cagggaatcc aatagcgtgt
    15841 gctgtggcgg tggaaagtat taatctcttt aaaaaggaag ggttgttgca aaagctccag
    15901 caaaagactg caatcttgaa aaacagttta gatcgattca aggagctaac tcacgtaggt
    15961 gaagtgcgtc agaaaggttt catggtcggt atagaacttg taaagagccg taagacaaaa
    16021 aggtcttatg cccctggaga caaaattggt caaaaggtca tctgggaggc aagaaaaaga
    16081 agtgtcatta tccgacctct gggtgacatt attgtactga tgccaccatt ggctatagac
    16141 aggcctaccc ttgaagaact tgttgatgtc acgtatgaga gcattaaggc agtcacaggc
    16201 agctaataga gaaagttata gataagagga ggggagtaac catgattcat gttattgctt
    16261 cagtaaaggt aaagcctggt aagcgtgatc aactgttgga tttattcagg gagaaccttc
    16321 ccagggtaag agaagagaag gggtgtatac gatattttcc cgcagtggat gttgccactg
    16381 gcttgccccc acaatcactg gatgaagata ttattactgt tattgaaacg tgggaaagct
    16441 tagaggcact gaaagatcat ctcgccgcgc ctcatatggc agcctttttt gaaaaagaga
    16501 aaccattagt atctggatca gttataaaga ttcttcagga agtatgaaac ctgcacaagc
    16561 gggttggctc tgtgttagtg ttctcttgtt ccgtatttcc cattgaacct gtatatgcgg
    16621 tgggcattct cgcaaatacg cctgttcact ccagcggctc gaatcgctcg tattcggctg
    16681 tgaaactttt gtaaacgcac aagaccgaac ttactccttc tgaacagttt gttcagaaga
    16741 accctacaca actatctatc ggcttatatg aactgattca ttttagaggc aacaatcagg
    16801 taggtatacg ggccgactcc taatgtcttt atctgttgga agccaaagct ggctatcaga
    16861 gtccctgtct cctctgggga tagccgaatc agtacaggtg gtccgggagg cccatcgatc
    16921 ttcttaaact cgacaactcc aagaataccc gctggtttga gcacccgggc agtttctttc
    16981 agagggactt caattccacc gtgtcgtata aagtcgtgca gcgctgctgc aatgagacag
    17041 atatctacgg tctggtcagg aaggggcaga tggtcggaaa cagatgcaac aaaagtctga
    17101 acattcgtga ggcgcctatc ctgagcgcgt ttttttaaaa tatctatgcc ttcagtccag
    17161 ccgtctatgc cataggcaat acctccggga cctattatct ctgcggctgc aataacgtaa
    17221 tcgccaattc cacaacccag gtcaagaaag atctttgatt tggtaagccc cagttcatgg
    17281 aagacgagtt cgtgatcaac gaggtggtag ctgctttttc cggaaccagg tagtttgctg
    17341 tatgacatga aaactcccct gttaattttg cattttttaa aatactctgt ccaggcttcc
    17401 cgcatagcct gtaagctctg cgtatccttc gacagttact gaaataccct ttgatatacc
    17461 ggatccagtc actgctccct cccaatatgt cacacctgtt gatgcctcag tcaccaactc
    17521 ctggctcgca acaattgggg taagggtgat atctattcct gttgatggta tcttgatccc
    17581 ccatctggca gggtaggtgc ctccgctttt tggacttttc caatgttctt taacattgag
    17641 cgatatttca ctctgtcgaa gatgacgtga tgaaccatct ttttcgatta tcgttcctga
    17701 agagcaagcc tcaatggttc catctttttt tcgtaattgg aagagcatga tgtccctgcc
    17761 atcagataaa tggataccaa accaatccca tccgacctgg tcagaaccaa gaacattcga
    17821 accgaactcc tggtcaaacc agcttatacc ggttactgca aaataattct tagataactc
    17881 ggtttgtatc caaccttttg ttgcaaggtc tgtgagtgaa taatagtatg atgcctgtcc
    17941 gggattgggt ccctttgtac tcagcccatt gtccccgtga aaaaccctgg gtttgcgtgg
    18001 cgaaatttcg aggtcgagcg ccattttatc catatgtgct ttaagatgaa tggtgttgcc
    18061 tatcatcttt gcggaccaat tcagggtcca tacatccaga ttacccagct ttgcgccggc
    18121 aagtccgggt ccgctgcgtg atgcctgatc ataataccaa aactttcctg atgtcctgtc
    18181 aacaatagca aagtgtgcaa ggtatacatc acgaatagac catggattat cgggccacgg
    18241 cgccacaagt gtaagcccct gtctgaaaaa agtcagctgg tatccatact ggttaccttc
    18301 tctgtcgcga agattacctg taaagtacca ccactcggta cgatactccg gatgcgctcc
    18361 atgatctctg ggaaatgacc actgccaggg cccaattgcc tgtttccaat ctttctccac
    18421 cgccattgct gttcttatcg gaaacacaaa ggcaattatt attaataaca ttataatcgc
    18481 tgatggattt gaacaatgcc ttatcatcct gcattcccta tgagctatca gctttgagct
    18541 atgagctgac ttcctgtcac ctgtcactaa atacctgcct cttttacatt tcctatagtt
    18601 tattacctct cgcccatggc cggatttttt tattcctccc ttatctgcat ctggggatag
    18661 gtccgacata ccttccataa cggatggagt gctgcaccga ggctggcaca gattgccacg
    18721 attccggtta caacgtaggg ctgccactcg ggataataaa aaacagtcca gttgaaactc
    18781 cgaagattga ttacctttat tagtattaaa gcaagagcag tccctcccac agtactcagg
    18841 agaaagctga ccaggcccat gccgagacct tcaagaacag tcataatacc aatctgggat
    18901 gtggagaatc ccagggcccg gtaaatgccg aaatcctttc tacgttcaag aaagagcgta
    18961 agcagggccc ctgtgatgcc aaaaaaagcg acgatgatag ccagaaagcg catagaacgg
    19021 gtgacggcga aggtgctatc gaagagtgcg aggatgtttc tttctagttg tttactcgta
    19081 aggacaggaa gatttcgttc ctgtgctttt ctgcgtatct ccccaagaag ttctgcccgc
    19141 tgtggattgc caggatcaat aaaaatgcca aggctgttga tcgtcgtgtc gccaaagatt
    19201 ttgatgtagg ttgagcgatc catcatgata agtccatgtt ctgtcgtgta atcgtaaaaa
    19261 atagctccta cagaaagtgc aacaggtcct tctataccgt ccagggtgac cctgtctccc
    19321 tcctttgttt tgaacctcct ggcaaagctc tcagagataa taaccccgcc att
//
LOCUS       DAOOMC010000046        19345 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdus sp. isolate AMR_MDS_2685
            NODE_2859_length_19345_cov_6.351166, whole genome shotgun sequence.
ACCESSION   DAOOMC010000046 DAOOMC010000000
VERSION     DAOOMC010000046.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864760
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdus sp. (wastewater metagenome)
  ORGANISM  Syntrophorhabdus sp.
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae; Syntrophorhabdus.
REFERENCE   1  (bases 1 to 19345)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19345)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,729
            Genes (coding)                    :: 2,708
            CDSs (with protein)               :: 2,708
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 7 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19345
                     /organism="Syntrophorhabdus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2859_length_19345_cov_6.351166"
                     /isolate="AMR_MDS_2685"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2699756"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..659)
                     /locus_tag="PK125_06245"
     CDS             complement(<1..659)
                     /locus_tag="PK125_06245"
                     /inference="COORDINATES: protein motif:HMM:NF012271.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HPB37742.1"
                     /translation="MMSLALVVLMGTGHAADVRLAWNASPEPDIAGYKIYYGTASRVY
                     DWSIDAKKVTTFTVSGLADGRTYYFAVTAYDTSNLESTYSNEVSTSTCRYSISPPGQS
                     FPASGGTGSINVSTQSACSWNASSNVSWMTITSGSRGTGNGTVYYSINANSDSSSRTA
                     SSTIAKNTFTVTQEGNSTTTYTITASAGTGGTISPTGTVTVSRNASRSFTITPSTGYS
                     I"
     gene            complement(1097..1675)
                     /gene="rdgB"
                     /locus_tag="PK125_06250"
     CDS             complement(1097..1675)
                     /gene="rdgB"
                     /locus_tag="PK125_06250"
                     /inference="COORDINATES: protein motif:HMM:TIGR00042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RdgB/HAM1 family non-canonical purine NTP
                     pyrophosphatase"
                     /protein_id="HPB37743.1"
                     /translation="MKNIIIATENMGKFREIKALLEHGFDKFYSLRDYKDRVPIEEDS
                     DLYIENALKKARKIGDRFGMHTLADDSGLEVQALGGRPGVRSSRYGKNDDERIARLLS
                     ELEGIPWDERAAVFKAYLAFYMPERERCYVFYGELRGIIGMDKHGQNGFGFDPVFFVP
                     ELNRYLAEIPMEKKNRLSHRGRAIQALIDFFH"
     gene            complement(1672..2400)
                     /gene="rph"
                     /locus_tag="PK125_06255"
     CDS             complement(1672..2400)
                     /gene="rph"
                     /locus_tag="PK125_06255"
                     /inference="COORDINATES: protein motif:HMM:TIGR01966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004549 - tRNA-specific ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HPB37744.1"
                     /translation="MRDNNRPNDKIRELKISKSFLKYPEGSVLVEMGETKVICGVSVE
                     EKVPPFLRNSGKGWLTAEYSMLPRSTHTRSVRESVTGRVGGRTHEIQRLIGRALRGIV
                     NLDIIGERTLWVDCDVIQADGGTRTASITGGYVALVDALWNLKKRGVIQKIPLRDSVA
                     AISVGLVGGEILLDLSYEEDSKAEVDMNFVMTGKGQLIEVQGTAEKTPFSKEQLDVMY
                     QYAHRGINEITRQQKIALGPMFPA"
     gene            complement(2390..2950)
                     /locus_tag="PK125_06260"
     CDS             complement(2390..2950)
                     /locus_tag="PK125_06260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB37745.1"
                     /translation="MRIKTFILIVIFFFAPVFYSHGNEHIVFYRLGAKDGVAWDQLKR
                     YLGAKGYRISIFDGADNIEKHVEIVNRINKLKASAFLAMDFGVGEKDQVMVAITRAKR
                     GKGSILAIDELPALYANESRELSSSLASVFDKGIKEFPLFPLLGVDMPGVFLKISFTP
                     EKTLEVFARLHEGMQRYFKRGMKDER"
     gene            complement(3141..4067)
                     /gene="trxB"
                     /locus_tag="PK125_06265"
     CDS             complement(3141..4067)
                     /gene="trxB"
                     /locus_tag="PK125_06265"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HPB37746.1"
                     /translation="MGEFHEAIIIGAGPAGLTAGIYLMRAGVESVLIEKGVMGGTPMN
                     YEHVENYPGFPGGISSPELMQKMAEQAKRFGLPIKEFIEVEEVNSEGDRFIIKAGADS
                     MESLGVIVCTGTVSVKMGIPGEEEFVGRGISYCATCDGAFFRNLDVAVIGGGDAAIQE
                     GLALTNIVRKVYVIHRRDQLRAQKIIQDRAFKNEKMEFLWNKVPVEIEGKDQVESILI
                     EDTKTHEHSRVKVDGVFVYVGARPNTQFLGNLVERDGAGFIVTDENLATKTAGLYVAG
                     DARKKPLRQITTAVGDGALAAVSLEHYIMETR"
     gene            4269..5057
                     /locus_tag="PK125_06270"
     CDS             4269..5057
                     /locus_tag="PK125_06270"
                     /inference="COORDINATES: protein motif:HMM:NF012596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HPB37747.1"
                     /translation="MSEPHTLYNVNNHVAIITLNRPKAKNAFSTEMISLWQEYLEEAR
                     KNDEIRVIIVTGKGDTFCSGGDIRDMAEGKLKSWDMKRFLWDGVHRIVLTLEDLDKPV
                     IAAINGAAMGAGMDMAIMCDMRVCSDRAKLAESYIMMGLVPGDGGAYFLPRLTGVSRG
                     LELLLTGDVIDPEEALRLGIVNRVVPHENLMEETMKLAAKIAAKPPLAIRMMKRAVYQ
                     GQTSTLRAHLDYISSQISLLSETHDHMEAATAFLEKRTPVFIGK"
     gene            complement(5385..6368)
                     /locus_tag="PK125_06275"
     CDS             complement(5385..6368)
                     /locus_tag="PK125_06275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB37748.1"
                     /translation="MVTGIGSFPFTDVDEAIDIIFETCPEIPFWPQLPKRAPYENMYI
                     PFLEGIPCVVVNENEGSAIMDTENAEGIEEFYDNVQGGNLEAFQISEKVAPGFYRFLD
                     RLRERTSAVRFIKTQITGPFSMGLGLKDETGKPVIYNYGYFDIIKKAIHMKAQWMLAT
                     IKGQFPDKGIIIFFDEPYMVSFGSAYVSISREEAIGIFNDVLGDIPGIRGIHCCGNTD
                     WSVLLNAQVDIINYDAFNYLDTIFYFDDDLRRFIKGGGRIAPGIVPSSEEVMKATPKD
                     MEHLMEKFQSLMIDKVGPEGLQNLIVTTSCGVGSLQPEHAKKAMELLKTFG"
     gene            6784..7230
                     /locus_tag="PK125_06280"
     CDS             6784..7230
                     /locus_tag="PK125_06280"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HPB37749.1"
                     /translation="MLIPTRILVPTDFSQYSDTALLYALDIAQEYKAQVYILHVVADR
                     IGHTIDDYGLTPRSIKQMEAKMIKGARNKLQKQIDKFPQTNTLEVFSEVVIGNPSEII
                     LNTQKDKKIDLIVISSLGKTGLAQYFLGSVVRNVLKGSTCSVLLTK"
     gene            7331..7777
                     /locus_tag="PK125_06285"
     CDS             7331..7777
                     /locus_tag="PK125_06285"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HPB37750.1"
                     /translation="MLRPTKILVPTDFSEYSDKALKQALDIGKQYNAKVFLFHVVSGE
                     IQRCVGDICLGDDIIRQIETQLKNKAQEDLQKQMNKFPQSKEVSVAMDIGHGIPHEAI
                     LREAEDKGIDLIVIASLGRSAIARFLIGSVARNVLKGAKCPVLLTK"
     gene            7901..8713
                     /locus_tag="PK125_06290"
     CDS             7901..8713
                     /locus_tag="PK125_06290"
                     /inference="COORDINATES: protein motif:HMM:NF015168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF255 domain-containing protein"
                     /protein_id="HPB37751.1"
                     /translation="MNKLANEKSAYLQHAAHQQINWYPWGDEPFEKAKQEDKPVFLSS
                     GAIWCHWCHVMAKESFEDEEVAKIINENYVAIKLDRDEMPDIDRRYQQVIAAMGFGGG
                     WPLSVFLTSDKKPFYGGTYFPPNERFGRPAFKTILMAIAELYRTQRAQVVESSEQLLN
                     MIKHKLLVSSEIQESIIDKGASTILKSADTLYGGFGRAPKFPMAGAIEFLPADIFLPE
                     MSPWQPCFGKHSPQWQGVGSTTTLPEGFTVTQLISPGLSPISRKWQMTMPGS"
     gene            8686..9858
                     /locus_tag="PK125_06295"
     CDS             8686..9858
                     /locus_tag="PK125_06295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB37752.1"
                     /translation="MADDNAWLLRNYVDAYSIFGDPFFKDVAQGIINFTRNELSHPDG
                     GFYASQDADVTPDDEGGYFTWTDNDFKALLTDEEYTVLSKYFLHERAMMHHDSSKRVL
                     YIDKNPEDVAGMIGMNVEKVNQIIIQGRRKLLHARETRTKPFVDTAMYSSLNGMYISA
                     FLRAYRVLRDSAIKDFALKSLERILTINTDNNTVFHTPGIKGLLDDHIHLIDALVSAY
                     EVTGKRPYLEKADLFMVQTIELFWDNDEGGFFDTSEEVLGTRLKGIDDTPHPSANSLA
                     IMLLIKLAFIRDKQEYRALAEKALKLFSSWGQEAGVHGGYYFSALDAFYNGLELTLNA
                     SPGTELAERALSTFRPYTAFAYRQEQEGNVTPCVRNVCYESIQEPDILQKFLSTPI"
     gene            10102..10174
                     /locus_tag="PK125_06300"
     tRNA            10102..10174
                     /locus_tag="PK125_06300"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10136..10138,aa:Glu,seq:ctc)
     gene            complement(10388..11545)
                     /gene="sfsA"
                     /locus_tag="PK125_06305"
     CDS             complement(10388..11545)
                     /gene="sfsA"
                     /locus_tag="PK125_06305"
                     /inference="COORDINATES: protein motif:HMM:TIGR00230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000023 - maltose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HPB37753.1"
                     /translation="MPLIRPLRLFKKPFQATFVSRPNRFLVECVLNENHVQAFLPNPG
                     RLQELLLPGATLYLNEESSSPLRKTSLTVIAVEREGVPVMLHTHRTNQVARYLIENGF
                     IQNLRGAEIIKAEATVGGSRFDFLLHDGRKEVYVEVKSCTLVGDKVAMFPDAVTARGA
                     RHLEELARLFAKGKRAVVLFIVHWPYAEFFMPDYHTDLYFAQTIARVRSAVEVIPAAV
                     HWNHDLSLEPGAKILHVPWEYIERESCDRGSYLIILSLAEDTRVNVGKLGTILFKKGF
                     YVYVGSAMANLTSRIERHKRIRKTFHWHIDFLRDSADFMYALAIRSSDRLECQVANGV
                     SKIAPWSIKGFGSTDCVCDTHLFGFADNPVSLPSFQKLLQHFRMDRYGEKD"
     gene            complement(11556..12173)
                     /locus_tag="PK125_06310"
     CDS             complement(11556..12173)
                     /locus_tag="PK125_06310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB37754.1"
                     /translation="MKRIPENTVHLIQEIDELWEPVYPFLAKHIVELYGRKDGVILEI
                     GPFCGAIYSLLLQGVGSQFVMAAFPPEMASFFGKQIHEKGHGEKICVVETDQSLSGIK
                     RDSIDLIVFRGALFFPSLFQVEYGAINRVIKKGGIAMIGGGFGKLTPPEIIRPIAEQS
                     KELNLKIGKVEVAADQIREDVKASGILTDFWIVQDGGLWVILNKG"
     gene            12363..13376
                     /locus_tag="PK125_06315"
     CDS             12363..13376
                     /locus_tag="PK125_06315"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008465 - glycerate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycerate dehydrogenase"
                     /protein_id="HPB37755.1"
                     /translation="MEPKVFVTRKIPESGINLVREYCEVTVYPGKGPLKGDELIQHAS
                     TMDGIICLPGDIIDKTFMSAAPHLKVVSTSSVGFEHIDVEEATRRGIYIGYTPGILTD
                     ATADLAFALLLSMARRIPQCDRFVRAFNWKAIGSPLHLFGTSVWGTTLGIIGLGRIGK
                     AMAERARGFHMRVLYNDNMRLTPAEEEDLHITYSTIDTLMQESDFVSLHTPLSSDTRH
                     LVNAEKLRLMKPSAILINTSRGAVIDEQALVQALKENWIAGAGLDVYEKEPLDPKNPL
                     LSLDNVVLVPHMGSATKQTRSAMSELAARNLLAVLHGTPPPSWLNPEVEKIRPLSEVK
                     MIP"
     gene            13541..14020
                     /locus_tag="PK125_06320"
     CDS             13541..14020
                     /locus_tag="PK125_06320"
                     /inference="COORDINATES: protein motif:HMM:NF012241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HPB37756.1"
                     /translation="MAAKESKEIARAEQPRSLQTLLDIDAMFDEFFRRPFSVFGLPRL
                     RFPETAELTPTVDIYEEKGDVVVKAELPGINKEDLDVTVSEDTITIAGEKKKEEEVKK
                     SDYYRWECSYGSFHRTFTLPAEVQTDKIKTKFKNGILEIRLPKTEESKKKEKKIKIE"
     gene            14192..15376
                     /gene="ahbC"
                     /locus_tag="PK125_06325"
     CDS             14192..15376
                     /gene="ahbC"
                     /locus_tag="PK125_06325"
                     /inference="COORDINATES: protein motif:HMM:TIGR04546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006785 - heme B biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="12,18-didecarboxysiroheme deacetylase"
                     /protein_id="HPB37757.1"
                     /translation="MIGISKLYCGAVEASDPLRYGRDSKRLPSHLLQFSADKKPVVVW
                     NMTRRCNLKCVHCYAHARDAFFKGNELNTEEGIRLIDDLAAFGSPVMLFSGGEPVLRE
                     DLPELIDYAVKKGMRAVISTNGTLITEEKARIFAKFNLSYIGVSLDGIGGVNDAFRGV
                     KGAFGKAMDGIRNAKKAGIKVGLRFTINKRNYKEIPRIFDLIDTEGIERACFYHLVYA
                     GRGSELMNEDLSHEESRKSVDYILDRTKSFFDKGKPIEVLTVDNHADGPYLYMRLLRE
                     DPARAAEVFDLLMMNEGNASGVGIACVDEEGNVHADQFWRHYSFGNVRKRPFSEIWMD
                     TSNELMDKLKRKKEFVKGRCARCRWLNICGGNFRVRAEAKTGDLWAEDPQCYLTENEI
                     AK"
     gene            15376..15801
                     /locus_tag="PK125_06330"
     CDS             15376..15801
                     /locus_tag="PK125_06330"
                     /inference="COORDINATES: protein motif:HMM:NF017451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HPB37758.1"
                     /translation="MVTGTGQQSMEKKGFEDTIVWQKAKELTVSICKITAENKAFEKE
                     FGLGDQIKRSSVSTAGNIAEGGERDTRKESIRFFYSAKGSLAEPRIQLEIPYSIQYIT
                     SGVFETLDSNCQTSGKNFSFISHRLSPLSFRPFMHSIVR"
     gene            15804..16784
                     /gene="hemB"
                     /locus_tag="PK125_06335"
     CDS             15804..16784
                     /gene="hemB"
                     /locus_tag="PK125_06335"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: protein motif:HMM:NF006762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HPB37759.1"
                     /translation="MNYPVYRPRRLRKNEKFRNMIQETELSPNHLIYPVFVKEMAEKK
                     VPIPAMPDVYQFSVEGLPQEIEEVASLSIPAVLLFGIPEKKDEVGSGAYAEDGVVQKA
                     VRVIKKLFGSDIMVITDVCMCEYTNHGHCGVIKDGEVDNDETLKLLARSAHSHVVAGA
                     DMVAPSDMMDGRVKAIRETLDISGFCTIPIMSYAAKYASALYGPFREAAESAPQFGDR
                     RGYQMDPPNQREALREIRLDIQEGADIIMVKPALFYLDVLSMAKQEFNIPLAAYSVSG
                     EYTMIKLAASKGYLDYERAVVEANTSIRRAGADIIITYFAKDLAGWYKTL"
     gene            16766..17779
                     /locus_tag="PK125_06340"
     CDS             16766..17779
                     /locus_tag="PK125_06340"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HPB37760.1"
                     /translation="MVQNTLRMVAWELTRNCNLNCVHCRAAASKGPHKGELTFAECKR
                     ILDQIAGFASPTIILTGGEPLMRDDIFEIIEYGNKQGLRLVIAINGTLLTEEKAMKLR
                     DGGIKRVSLSLDGKDAKAHDAFRGVDGSYNSVMKAADILNATGLPFQINTTVTRFNVN
                     DLFEIYNLVKLIGAVAWHVFLLVPVGRGKDLKGKELDAMAYEDVLNWLYDIESRNELE
                     MKVTCAPHYYRIVKERGSIPKSAGCLAGKSFMFISHKGIAQPCGYLEISSGDVRAHGV
                     KKVWEESPVFNQLRDISSYRGKCGGCRYLKICSGCRARAYELHGDFLQEEPYCSYGHP
                     AEE"
     gene            17907..18737
                     /locus_tag="PK125_06345"
     CDS             17907..18737
                     /locus_tag="PK125_06345"
                     /inference="COORDINATES: protein motif:HMM:NF024946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HPB37761.1"
                     /translation="MMMEGIDHTTSWTAQITCMKWTISVLFIIWLLLTPQPSSGARPL
                     VTDDADTAGKGKAQIELGVEIFTWQDRERGIKTRETGTEISSTFTYGVADHLDLVVGI
                     PYVWGAMEKNGQTTFDADRLSDISLEVKWRFYENKVFQVAFKPGLTLPTGNHRKEFGT
                     GRPTYGLVFIASKEMGDMGFHFNTCYTRNENKVDKRVNLWSASVAATYEAIKGLNLVG
                     NVGLKHNTDPAIKTAPAFWLAGFNYSFNDYFTIDGALKFRLNRQEADRAMIAGITIRF
                     "
     gene            18895..19338
                     /locus_tag="PK125_06350"
     CDS             18895..19338
                     /locus_tag="PK125_06350"
                     /inference="COORDINATES: protein motif:HMM:NF013629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HPB37762.1"
                     /translation="MVPTYRERYQAMVKKLKDNSHKLTPQRLAIIRILAGSENHPSVE
                     DIYNLLHKRFPGISQATVYRNIMLIKSLGEVLEIGFAGASTRYDGIKPFPHPHVVCLK
                     CNKIIDPELASLHDMTKEITDESGFEIVTFRLDFFGVCPECRAMG"
BASE COUNT         5308 a         4614 c         4390 g         5033 t
ORIGIN      
        1 tcgatgctgt accctgtgct gggtgttata gtgaaggacc ggcttgcgtt cctgctgacc
       61 gttactgtgc cggtgggtga gatggtgcct ccagttccgg cagaggcagt gatggtatag
      121 gtggtggtgc tgttaccttc ctgtgttact gtaaatgtat ttttcgctat agtggaactg
      181 gctgttctgg aagatgaatc gctattagca ttaatggaat aatataccgt accattcccg
      241 gttccgcgac tccctgaggt gatagtcatc cacgaaacat tactgctggc attccaggaa
      301 catgcagact gcgtgctcac attaatagag ccagttcctc ctgatgcagg aaaactctga
      361 cctggaggcg aaatggaata tctgcatgta cttgtgctta cctcgttgga ataggtactc
      421 tcgagatttg acgtgtcgta ggcagtaact gcaaaataat aggttcgccc atcggcaagg
      481 cccgatactg taaaggttgt taccttttta gcgtcgattg accaatcata gacacgagat
      541 gctgtaccat aataaatttt ataacctgct atgtccggct ccgggcttgc gttccaggca
      601 agccgtacat ccgctgcatg ccccgttccc atgagcacca cgagggcaag actcatcata
      661 aaagtgaaca gcagaagatt tctgattata ttattcctat tattacagta tgttagagct
      721 tttaatacag gcaaaacaac gtcctctgat aaagtttctg accctctcca gaaagaattt
      781 ctgtaatcct tcttttccgc atgtgtaatt ggtttcgcaa gcaaggcctg tgccaatgta
      841 gctacgagtt atattagtat gatatcattg atgttttata ttacaaatcc attttgttct
      901 cgaaagagcg catggtttca ataacatatt gcctgtggcg aatgtaggaa tttccctaca
      961 tccatatgtt cttacaccta agttcctaat ttatcattat aaatggtgaa aaccgttcac
     1021 aaccggggag ggaatgttga ggaattgtaa tgctggtgaa tggcccaaac aggccatttt
     1081 gtattttttt gtgcctttaa tgaaaaaagt cgatcagagc ctggatcgct ctaccgcggt
     1141 ggcttaagcg attttttttc tccatgggaa tttcggcaag gtatctgttc agctcaggga
     1201 caaagaagac aggatcaaaa ccaaaaccat tctggccgtg cttatccata ccgataatcc
     1261 ctctcaactc gccatagaaa acatagcacc gttccctttc gggcatatag aaagcgaggt
     1321 aggccttgaa aacagcagcc cgctcatccc aggggatacc ttcaagttct gagagcagcc
     1381 tggcaatcct ctcatcatca ttcttgccgt agcgggacga gcgaaccccc ggtcttcctc
     1441 ccagcgcctg tacctcaagg ccagaatcat cagccagggt gtgcatgccg aacctgtcgc
     1501 ctattttcct ggcttttttc agggcattct caatataaag gtcgctgtct tcttcaatgg
     1561 ggaccctgtc cttgtaatca cgtaatgagt agaatttgtc gaaaccatgt tccaggagag
     1621 ccttgatttc ccggaatttt cccatgtttt ctgttgcgat aatgatgttt ttcacgccgg
     1681 aaacattggc cccagggcaa tcttctgctg tcgggtgatt tcattgatcc ccctgtgcgc
     1741 atactggtac atgacatcaa gctgctcttt tgagaatggg gtcttttctg cagtaccctg
     1801 cacctctatg agctggcctt ttccggtcat cacaaaattc atgtccactt ctgccttcga
     1861 gtcctcttca taggagaggt caagaaggat ttctccgcct acaagcccca cgctgattgc
     1921 cgctaccgag tcgcgcaggg gtatcttctg gatcaccccc ctctttttga ggttccacag
     1981 ggcatcgaca agggccacgt agcctccggt gatgctcgcc gtccttgtcc cgccgtcagc
     2041 ctggatcaca tcacaatcaa cccagagcgt tctttccccg attatgtcaa gatttacaat
     2101 tccccggagc gctcttccga taagcctttg aatctcatgg gtccgtccgc caacgcgccc
     2161 tgttaccgac tcacggactg acctggtgtg ggttgaccgc ggcaacatgg agtattcagc
     2221 ggtaagccag cctttcccgg agtttcgcag aaaggggggt accttttcct ccacactcac
     2281 cccacaaatc acctttgtct ctcccatctc cacaagaaca gagccctcgg gatacttaag
     2341 gaagctcttg gatatcttca gttctcttat tttgtcattc ggcctgttgt tatctctcat
     2401 ccttcatacc tcgcttaaaa tacctttgca taccttcgtg gagccgggca aacacctcaa
     2461 gcgttttctc gggtgtaaag cttattttca ggaaaacacc gggcatgtca accccgagca
     2521 gcgggaaaag agggaactct ttgatcccct tatcaaacac agaggcgagc gatgatgaca
     2581 gctcccgcga ttcattcgcg taaagggcag gcagttcatc gatggccagt atagaaccct
     2641 tccctctttt tgcccttgtg atcgctacca tcacctggtc tttctcgcca acgccaaaat
     2701 ccatggctaa aaaggccgag gcttttaact tgttgatacg attgactatt tccacgtgtt
     2761 tttcgatgtt gtccgcccca tcaaatatgc ttattctgta ccctttcgca ccgaggtacc
     2821 ttttcaattg atcccaggca acaccatctt ttgccccgag acgataaaat acaatatgtt
     2881 cgttcccatg ggaatagaaa actggtgcaa agaaaaaaat aactatgaga atgaatgttt
     2941 ttatgcgcac aaagcattac cctttatagc tattggaagc ttgcatgctt gcttaaacca
     3001 gagggaagaa ggaagcccgg ttgccattca cgcattttcc gctccgcctt tcgtgttcca
     3061 cagttgtctt ttcccatgac cctttaccta taacctggct ttttgataca tttcaacatt
     3121 tcaagaacgt ccccgaagtt ttatcgggtt tccatgatgt agtgttccaa actaacggcg
     3181 gccagtgcgc catctcccac cgctgtggtt atctgccgca gaggcttctt tctcgcatcc
     3241 ccggcaacat acaggcctgc tgtttttgtg gccaggtttt catcagtgac aataaaacct
     3301 gccccatccc gctccacaag gttaccaagg aactgtgtat tcggtcgggc acccacatag
     3361 acaaacacac catcaacctt tacgcgggag tgctcatggg tttttgtatc ttcaatcagt
     3421 atggattcca cctgatcctt tccttcaatt tcaacaggaa ccttgttcca cagaaattcc
     3481 attttttcgt ttttgaatgc cctgtcctgg atgatcttct gcgccctcag ttggtctctg
     3541 cggtggatca cgtacacttt gcgaactatg tttgtcaggg caaggccttc ctggattgcc
     3601 gcatcgccac cgccgataac tgccacatca aggtttctga aaaaggctcc atcgcaggtg
     3661 gcgcagtatg agatgcccct gcctacaaat tcctcctctc ccggaatgcc catcttcaca
     3721 gaaacagttc cggtgcatac gatcaccccg agggactcca ttgaatctgc gccagctttg
     3781 atgataaatc tatctccctc actgttgacc tcttcaacct cgataaactc ttttatggga
     3841 agaccaaagc gttttgcctg ttcagccatc ttctgcatga gttctgggct tgatatgcct
     3901 ccgggaaagc cgggataatt ttcaacatgt tcatagttca tcggtgtgcc gcccatgacc
     3961 cctttttcta tgagcacgga ttcgacccct gccctcatca ggtaaatacc tgcagtcaat
     4021 cccgcgggac ctgcccctat gattattgcc tcatggaatt cgcccacttc aacctcctaa
     4081 gatccgtaac gcgtgacgcg taatccgtga ttgatattct ttaaaaaccc gttacgcgtt
     4141 acttatcacg cgtcacagct tttgccgcta ttttaagcaa tccccattta atattcaaat
     4201 agtttttcct tgacacaaat gccggacgtg ggcgatttta gtaaaaaaat cagaaaagga
     4261 caaacaccat gagcgaacca cacacgctat acaacgtaaa caaccatgtt gccataatca
     4321 ccctgaacag gccaaaggca aaaaacgctt tcagcaccga gatgatatct ctgtggcagg
     4381 aatacctgga ggaggcaaga aagaatgatg agatacgggt tatcattgtc acaggcaagg
     4441 gcgacacctt ctgttctgga ggggatatac gagacatggc agaaggcaag ctgaaatcgt
     4501 gggacatgaa aagatttctc tgggatgggg ttcaccgcat tgtcttgacc cttgaagatc
     4561 tggacaaacc ggttattgcc gctatcaacg gcgctgctat gggtgctggc atggacatgg
     4621 ccatcatgtg cgacatgagg gtatgctcgg acagggcaaa actggctgaa tcctacataa
     4681 tgatgggcct tgtacccggt gacggcggag cgtattttct gcccaggctc acaggcgttt
     4741 ccaggggcct ggagcttctg ttaacggggg atgttataga tcctgaggag gcattacggc
     4801 tcgggattgt gaatcgtgtc gttcctcacg aaaaccttat ggaagaaacg atgaagctcg
     4861 ctgcaaagat tgccgccaaa ccgccccttg caattagaat gatgaagaga gccgtctatc
     4921 agggacagac cagcacattg cgtgcccacc ttgactacat ctcttcccag atctcactcc
     4981 tttcagagac acacgaccac atggaggcag caacagcatt ccttgaaaaa cgcacgcctg
     5041 tgttcatagg aaaataaaac cttgggggac gttcttgaaa tgttgaaatg ttttcccctg
     5101 gataaaatta tccatatttt gccacggata gacgcagaga aaagcagatc tcccgtctgc
     5161 ttcgcccggg cacacaaaat acacagagac ggttctcttc tttcttgcct gattttacaa
     5221 aaaaacctga atcaggtggg atcgcgcgcc atgcactgaa agcattccct ccaatgtatt
     5281 gcaacagaaa aggatgcatc aatctgaaag cgctgcggcc tcggacgaac gtatgtgacc
     5341 gtgcgtgtgg agataagctt gtacaggctg acgacgaaat atggtcatcc aaaagtcttc
     5401 agaagttcca tagccttttt ggcatgttcc ggctgaagac tgccaacacc gcagcttgtg
     5461 gtaacgatga ggttctgcag cccctcagga ccgaccttat ctatcattag ggactgaaac
     5521 ttctccataa ggtgttccat atcttttgga gtagctttca taacctcctc ggatgaaggc
     5581 acaatacccg gggcaatcct gcctccccct ttgataaacc ttcgtaaatc atcgtcaaag
     5641 tagaagattg tgtccaggta attaaaggca tcataattga tgatatccac ctgtgcattg
     5701 agaagaactg accagtcagt attgccgcag caatggatac ccctgatgcc aggtatgtcc
     5761 ccgagcacat cattgaatat gccaatcgcc tcctctctgg agatggaaac ataggcagaa
     5821 ccaaaagaga ccatataggg ctcgtcaaaa aaaattatga tccccttgtc aggaaattgc
     5881 ccttttattg tggcgagcat ccactgtgct ttcatgtgaa tggctttttt gatgatatca
     5941 aaatacccgt agttgtaaat aacgggcttt ccggtttcat ctttcaaacc aagtcccatg
     6001 ctgaacggac cggtaatctg agtcttgatg aatctgacgg cactggttct ctccctgaga
     6061 cgatcgagaa acctgtagaa accaggggca accttttcag aaatctgaaa ggcctcgagg
     6121 ttaccgccct gaacattgtc atagaactct tctatcccct cagcgttctc cgtatccatg
     6181 atggctgacc cttcattttc atttacaacc acacacggta tgccttcgag aaaagggata
     6241 tacatgttct catagggtgc acgttttggc agttgcggcc aaaaagggat ttctgggcat
     6301 gtttcgaata ttatatcaat cgcttcatct acatctgtaa aaggaaaact gccaataccg
     6361 gtaaccataa agactcctta aaacgggcat cgagtgatag gtaagatcaa aaccattcgc
     6421 ttatctcctg ctgctggaaa attataatct gtcatcgaaa aaacttcaat tgtgatttat
     6481 ttttcacgat tcccgccctt gttccgcttt atcttaaaca tttcagcacc agttccctat
     6541 caagtaacat ctctgattaa ccccgggcag attcaacagt ttgatcggtt ctgagacggt
     6601 ccagaaaaga tgcttatttc ttagggaata cttttttaat ggtgttcctg ggaccgatta
     6661 tgcccgggtt cacagccgct cccgggtggt tgacagccgt gatattactg ctattattat
     6721 agtatgagga tttaaataaa ggttatcggg aataagaaga tagcctttaa aaaggaggta
     6781 catatgctaa taccgacaag aatccttgta cctaccgatt tctcccagta ttcagacacg
     6841 gccctcttat acgctcttga tattgcccag gagtacaaag ctcaggtcta cattctccat
     6901 gtggttgctg ataggatcgg tcacaccata gacgactacg gactgacacc ccgatcaatc
     6961 aaacagatgg aagcaaaaat gattaaaggg gccagaaata agcttcaaaa acagatcgac
     7021 aagttcccac agacgaacac cctggaagtc tttagtgaag tagtgattgg aaatccttcc
     7081 gaaatcattt tgaataccca gaaagacaag aagattgatt tgattgtcat ctcgtcactg
     7141 ggcaaaactg gtctggcaca gtatttcctt ggcagcgtgg ttaggaatgt tctcaagggc
     7201 tcaacctgct ctgtgctgtt aacaaaataa cgggggatta tcatggcagg ctttggttcc
     7261 tgcaggatgt caacaggatc ggcaattcca aatttatccg cattctttta tttgaaagga
     7321 gggacaggat atgctacgac caacaaagat tcttgttcca accgattttt cagaatattc
     7381 tgacaaggct ctgaagcagg cgctggatat tgggaaacag tacaatgcca aggtatttct
     7441 ttttcatgta gtttctggag agattcaacg ttgcgtggga gatatatgcc tgggtgatga
     7501 tattatccgt caaattgaga cccagctaaa aaacaaggcg caggaggatc tccaaaaaca
     7561 gatgaataaa tttcctcagt ccaaagaggt atccgttgcc atggatattg gccatggaat
     7621 accccacgag gctatcctta gggaggcaga agacaaagga atcgatctca ttgtcattgc
     7681 gtccctcggg agatctgcca tagcaaggtt tctcatagga agcgttgcac gaaatgttct
     7741 caaaggagcc aaatgcccgg tacttctcac gaagtagttc aggattgaga gagtaaggtt
     7801 gtagattaaa aacacatatt gccaatcccc ctattcgtgt ttacctttaa gcaacagggt
     7861 tgagctttaa atctcccccc atttcagaaa aggagcatcc atgaacaagt tagccaatga
     7921 aaaatcagcg tacctccagc atgctgccca tcaacagatc aactggtatc cctggggaga
     7981 tgaacccttt gaaaaggcaa aacaggaaga caaacccgtt tttttgagct cgggggcaat
     8041 ctggtgccac tggtgccatg tcatggcaaa ggaaagtttt gaagatgaag aggttgcaaa
     8101 gatcattaac gaaaactatg tggccataaa actcgacaga gatgaaatgc ctgatataga
     8161 cagacggtac cagcaggtta tagctgccat gggttttggc ggcggctggc ctctgagcgt
     8221 gttcctgaca tcagacaaga aaccatttta tggcggcaca tactttcctc caaacgaacg
     8281 ttttggaaga cctgcgttta aaacgatact catggctata gctgaactct accgaacaca
     8341 gcgcgctcag gtagtggaga gcagcgaaca gcttctcaac atgatcaaac acaagctgct
     8401 tgtctcctct gaaatacaag aatcgatcat tgacaaaggc gcaagcacaa ttctgaaatc
     8461 tgcagatacg ctctacggtg gatttggtag agcgccaaaa tttcccatgg caggggctat
     8521 cgagttcctc ccagcagata tttttttacc agagatgagt ccctggcaac catgcttcgg
     8581 aaaacactca cctcaatggc aaggggtggg ttccacgacc accttgccgg agggtttcac
     8641 cgttactcaa ctgatcagtc ctggattgtc ccccatttcg agaaaatggc agatgacaat
     8701 gcctggctcc tgagaaatta tgtggatgca tacagcatct ttggcgatcc ctttttcaaa
     8761 gatgtggcgc agggcatcat aaacttcacg agaaatgagc tgtcacatcc tgatgggggt
     8821 ttctatgcaa gccaggatgc tgatgtgacg cctgatgatg agggaggata cttcacctgg
     8881 actgacaatg attttaaagc actgctcacc gacgaagaat atactgttct ctccaaatac
     8941 ttcctccatg aacgggccat gatgcatcac gattcgtcaa aacgggtact gtacatagat
     9001 aaaaaccctg aggacgtggc agggatgatt ggaatgaacg tcgaaaaggt caatcaaatc
     9061 attattcagg gcagaagaaa actcctccat gcccgtgaaa ccagaacaaa accttttgtc
     9121 gatactgcca tgtactcatc cctgaacggc atgtatatct ctgcttttct acgggcttac
     9181 cgtgtactca gggattcagc cattaaagac tttgccctta agagccttga aagaatcctc
     9241 accattaaca ctgacaataa caccgtgttt cacacccccg gcataaaggg tcttctcgat
     9301 gatcacatcc atcttatcga tgctcttgtg agtgcctatg aagtcacagg caaaagaccc
     9361 tatcttgaaa aggcagacct attcatggtg caaacaatag aactcttctg ggataatgat
     9421 gaagggggct ttttcgatac ctccgaagag gtccttggca caaggcttaa gggcattgac
     9481 gacacacccc atccatctgc aaattcactt gcaatcatgc tactcataaa acttgctttt
     9541 atcagagata aacaggaata tcgggccctt gctgaaaagg cactcaagct gttctcttcg
     9601 tggggacagg aagcaggcgt tcatggtgga tattatttca gcgccctgga tgctttctac
     9661 aatggacttg agcttaccct taacgcatct ccaggaactg agctggcaga aagagccctg
     9721 tcaacatttc gcccctatac agcctttgca tacagacagg aacaagaagg aaacgtcaca
     9781 ccctgtgtca gaaacgtatg ctacgaatca atacaggagc cggacatcct ccagaaattc
     9841 ctgtcaacgc caatataagc tcatagctga aagctccaag gtgtgcgcaa aggctcaaaa
     9901 ggataattgc agttctgcat ttccttacag ccgagggctt tacccttctt ttttctttct
     9961 ttcacattgc tcctgtattt acttttcact tttcactttc tttcctgccc ttcccattga
    10021 ccctcgcctc acacctatcc cgtgccttta tattattttc tggaaatcct ttttcatttc
    10081 tgctaatatt cttttctcaa tggtcccata gtctagtggc ctaggacgct ggcctctcac
    10141 gccggaaacg ggggttcgaa accccctggg actatttctt agaatatcaa taagttgcaa
    10201 gaatagtcag gttgcatcaa ttatgtagct aaaatgtctg ttcagcacca ggcctaatct
    10261 tcagaaaaca atataaaatg gatgaggaag cctgatcttt gtcgagagcg ttttttttat
    10321 aaggtaaatc acatccgtta ctggggccat gagagcagaa gtaacgaatc gtacatggga
    10381 tcggttatca gtctttttcc ccgtatcggt ccattctgaa atgctgaaga agcttctgga
    10441 aagacggcag gctaaccgga ttgtccgcga atccgaatag atgggtatca caaacacagt
    10501 cagtggaacc aaaccccttt atcgaccacg gagcaatctt tgatacccca ttggctacct
    10561 ggcattccag acggtcagaa gagcgaatgg caagggcgta catgaaatcc gcagagtctc
    10621 tcagaaaatc aatgtgccag tgaaaagttt ttcggattct cttgtgccgc tcaatccgtg
    10681 aggtgagatt agccatggca gagcccacat acacgtaaaa accttttttg aacagaattg
    10741 ttcctaattt gcccacgttt actcttgtat cctctgccag gctgaggatg atgagataac
    10801 tacccctgtc acaagattca cgctctatgt attcccatgg aacatgcaga attttcgcac
    10861 ccggttccaa agaaagatca tgattccagt gaactgccgc aggaatgact tcaacggcac
    10921 tgcgcactct ggcgatggtc tgggcaaaat aaaggtctgt atggtaatcc ggcatgaaaa
    10981 attctgcgta aggccagtgt actatgaaga gtacgacagc tcgtttcccc ttggcaaaaa
    11041 gtctggcaag ttcttccaga tgccgtgccc ctcgtgccgt gactgcatcg ggaaacatgg
    11101 caaccttatc tcccaccagg gtgcacgatt taacctccac ataaacttct ttccttccat
    11161 cgtgcaaaag aaagtcaaag cggcttccac ccactgttgc ctctgccttg ataatctctg
    11221 cccctcgaag gttctgtatg aatccattct ctataagata tctcgcaacc tgatttgttc
    11281 ggtgggtatg aagcattact ggaacacctt cccgttcaac agcaataacg gtaagggagg
    11341 tctttctcag ggggcttgaa ctttcttcat tcagataaag cgtagcccct ggcagaagga
    11401 gctcctggag cctcccggga ttcgggagaa aagcctgtac atgattttca ttcaacacac
    11461 attccaccag aaagcggttt ggacggctga caaacgtagc ctggaatggt tttttaaaaa
    11521 gcctgagagg tctaataaga ggcataggtt tgaggtcaac ccttgttcag gataacccag
    11581 aggcctccat cttgaacaat ccaaaaatct gtcaatatgc cgctcgcctt gacatcttct
    11641 ctgatctggt cagcagcaac ttcaaccttg ccaatcttca gatttagttc ttttgattgt
    11701 tctgcaatag ggcgaattat ctcaggaggg gttaactttc caaaaccacc cccgatcatg
    11761 gcaattccac ctttttttat gaccctgttt atagctccgt actcaacctg aaaaagggat
    11821 gggaaaaata gcgccccccg gaaaacaatg agatcgatgc tatctctttt tataccggag
    11881 agggattggt cagtctcgac aacacaaatc ttttcaccat gccctttctc gtgaatctgc
    11941 ttgccgaaaa aagatgccat ctcaggcgga aaagcagcca tcacaaactg gctgccaaca
    12001 ccctgcaaca gcaatgaata tatggcaccg caaaaaggtc ctatttcaag aatcactcca
    12061 tcttttctgc cgtacaactc aacaatatgt tttgccaaga atgggtatac tggctcccac
    12121 aactcgtcaa tttcctgaat aagatggacc gtgttttcag ggatacgttt catgtcgttc
    12181 aaagccccca cagtatatga gagttctcaa atcctgtcaa cggcatgtta tacttgaggg
    12241 cccatagtac actcagtaag gagatgattc ttgttgaatt tatctcctgt tacacgtatt
    12301 gtttctctgc atgtgggaac tgaactacac cataccaaca tccattcctg aaggaggaga
    12361 atatggagcc aaaggtcttt gttaccagaa aaatccctga atcgggcatc aacctggtac
    12421 gtgaatactg cgaagtaacc gtctatcctg gcaaggggcc gctgaaaggc gatgagttga
    12481 tacaacacgc ctccactatg gatggcatta tctgtcttcc cggggacata atagacaaga
    12541 cttttatgag tgcagcaccc catctcaagg ttgtcagtac ctccagtgtt ggatttgaac
    12601 atattgacgt tgaggaggca acacgaaggg ggatctacat cggctacaca cctggcatac
    12661 tcaccgatgc aactgctgat cttgcttttg ccctcttgct cagcatggca agaagaatcc
    12721 cccagtgcga tcggtttgtt cgtgcattta actggaaagc tatcggttca cctcttcatc
    12781 tctttggaac ttcggtttgg ggaaccacac tcggcattat tggccttgga aggattggaa
    12841 aggccatggc tgaaagagcc aggggtttcc atatgagagt gctctataac gacaatatgc
    12901 gcctcacccc agcggaagaa gaagaccttc atataacata cagtaccatt gatacactta
    12961 tgcaggaatc agattttgtt tcacttcata cacccctcag ctcagacacg cgacatctgg
    13021 tcaatgcaga aaaattgagg ctaatgaagc cctcggctat tctcataaac acttcccgcg
    13081 gcgctgtaat cgatgaacaa gccctggttc aggccctcaa ggagaactgg atagctggtg
    13141 ctggcctcga tgtgtatgaa aaagaaccgc tcgatccgaa aaatcctctc ctttctctgg
    13201 acaacgttgt gctcgtgcct catatgggga gcgccaccaa acagacccgt tcagccatgt
    13261 ctgagcttgc agcgcgcaat cttcttgcag ttcttcatgg cacaccccct ccttcgtggt
    13321 taaaccctga agtggaaaaa atccgtcccc tctcagaagt caagatgatt ccttaaaaaa
    13381 cgcatagagc atagaggaaa atactggaca gcagacaaag gctgctggtt gaattggcag
    13441 caggctacaa ggttcgccct ggggttgaca attttgcata ccatgactat cctgtattta
    13501 gtattaacaa caataattac caatatttag gaggtgccct atggcagcaa aagaatcgaa
    13561 agaaatagca agagcagaac aaccaaggag cctgcaaacc cttttggata ttgatgctat
    13621 gtttgatgaa ttttttcgga ggcccttttc tgtatttgga ctaccaaggc ttcgttttcc
    13681 tgaaacagca gaactaacgc caacagtaga tatttacgag gaaaagggtg atgtggtggt
    13741 aaaggcagaa cttcctggaa tcaataagga ggatcttgat gttacagtgt ctgaagatac
    13801 cattaccata gccggtgaaa agaagaaaga agaggaggtt aaaaaatctg actactacag
    13861 gtgggaatgc tcctatggtt cattccacag aacatttacc cttccggctg aggtacagac
    13921 tgataagatc aagacaaaat tcaagaacgg tatccttgag ataaggttgc caaaaaccga
    13981 ggagtcaaaa aagaaggaga aaaagataaa aatagaataa tctttttcag ggcctcatat
    14041 tctgattcag ttcacatgag gccctctccc acctgtctca ctcgccctgt gttgtctacc
    14101 cttaccacgt actcttcact tttacttttt actttcactt cttactccct ttcccgttga
    14161 cttttgctcc atttttgctc aaaatacatg ggtgattgga atatcgaaac tatactgtgg
    14221 agctgttgag gcgtctgatc cgctcaggta tggcagggat tcaaagagac ttccttccca
    14281 cctcttacag ttctctgcag acaagaaacc ggttgttgtc tggaacatga ccaggcgatg
    14341 caatctgaaa tgtgtccatt gctatgccca tgcgagggat gcctttttca aaggcaacga
    14401 gctcaataca gaagaaggaa tacgcttaat cgatgatctt gctgcttttg gttctccggt
    14461 aatgctcttc tcagggggag aaccggtcct ccgagaggat ttgccagagt tgatcgacta
    14521 cgcagtaaag aaagggatgc gggcggtcat ttccacaaac ggaaccctta tcaccgagga
    14581 aaaggcacga atctttgcaa agttcaatct ttcttacatc ggtgtcagtc tcgacggtat
    14641 cggtggcgtg aatgatgcct tcaggggtgt gaagggcgcc tttggcaaag ccatggatgg
    14701 catcaggaat gcaaaaaaag caggcatcaa ggtcgggctc cgatttacca tcaacaagcg
    14761 caactacaaa gaaatcccca ggatatttga tctcatagat acggaaggga ttgaacgggc
    14821 gtgcttctac catctcgtgt atgcaggacg cggatcagaa ttgatgaatg aagatctcag
    14881 ccacgaagag tctcggaagt cagtggacta catcctcgat cgtacaaaat cgttttttga
    14941 taaaggaaag cccattgaag ttttgaccgt tgacaaccat gctgacggcc catatcttta
    15001 catgcgcctc ctcagggaag atccagcgag ggcagctgag gttttcgacc ttctcatgat
    15061 gaatgaagga aatgcttctg gagtcggcat agcctgcgtg gatgaggaag gcaatgtgca
    15121 tgctgaccag ttctggcgac attacagctt tggaaatgtt cggaaaaggc cattcagcga
    15181 aatatggatg gatacctcaa atgaactaat ggataagctg aaaagaaaaa aggagttcgt
    15241 gaagggaaga tgcgccaggt gccgatggct gaacatatgt ggcggcaact tcagggtaag
    15301 ggccgaagcc aaaacagggg atctgtgggc cgaagacccg cagtgctatc tgaccgaaaa
    15361 tgaaattgcc aaataatggt aacaggcact gggcaacagt ctatggaaaa aaaaggcttt
    15421 gaggatacca ttgtgtggca aaaagccaag gagcttactg tatcaatctg taagattaca
    15481 gcagaaaaca aggcttttga aaaagaattc ggcctgggag atcagataaa aagaagctcg
    15541 gtaagcacag cgggcaatat tgcagagggt ggtgaacgag acacccgcaa ggagtcaata
    15601 agatttttct atagtgcaaa aggatcattg gcagaaccgc gcatacagtt agaaatcccc
    15661 tattcgattc aatacataac ttccggtgtt tttgaaactt tggattcgaa ctgccaaacg
    15721 tccgggaaaa atttttcttt catttcccat cgcctctcac cactctcctt tcgcccattc
    15781 atgcattcta ttgtgaggtg aacatgaatt accccgttta caggcctcgc cggttaagga
    15841 aaaatgagaa attcagaaac atgattcagg aaacagagct atccccgaac catcttattt
    15901 accctgtctt cgtaaaggaa atggccgaga aaaaggttcc catccctgcc atgccagatg
    15961 tgtatcagtt ctcggttgaa gggcttcccc aagagataga ggaggttgcg agcctttcca
    16021 ttcctgcagt tcttcttttt ggtatcccgg agaagaaaga tgaagtggga agcggtgcct
    16081 atgctgaaga tggggttgtt caaaaggcgg ttcgtgttat aaagaagctc ttcggcagcg
    16141 acatcatggt aataactgat gtgtgcatgt gtgaatacac aaatcacggt cattgcgggg
    16201 ttatcaagga cggtgaggtt gataatgacg aaactctcaa gctccttgcc agaagcgccc
    16261 actcacacgt tgttgccggt gctgacatgg tagccccttc tgacatgatg gatgggaggg
    16321 tgaaagccat tagggagacc cttgatatta gcggattctg cactatccct atcatgagct
    16381 atgctgcaaa gtacgcctca gcactttacg gccctttcag ggaggcggca gagtcagccc
    16441 cccagtttgg tgaccggcgc ggatatcaga tggatccccc aaaccagaga gaagccctcc
    16501 gggagatcag actcgatatt caggaaggtg ctgacatcat catggtaaag cctgcactct
    16561 tttatctcga tgtcctttcc atggcaaagc aagaatttaa catccctctc gcagcttatt
    16621 cagtaagtgg cgagtataca atgatcaaac tcgccgcatc aaaaggatac cttgactatg
    16681 aacgggctgt tgtcgaggca aacacctcaa tacggagagc aggcgctgat ataatcataa
    16741 cctattttgc aaaggacctt gccggatggt acaaaacact ctgagaatgg ttgcatggga
    16801 acttacccgc aactgcaacc tcaactgtgt ccactgcagg gcagcggcat ctaaaggtcc
    16861 ccacaaagga gaattgacct ttgccgagtg caaacgtatc ctcgaccaga ttgccgggtt
    16921 tgcctcccca acaattatcc ttacgggtgg tgaaccgctc atgcgggacg acatcttcga
    16981 aatcattgag tacggaaata agcagggtct gaggcttgtt atagccataa acggtaccct
    17041 cctcaccgag gaaaaggcca tgaagctcag ggatggtgga atcaagcggg taagccttag
    17101 cctcgatggc aaggatgcga aagcccacga tgccttcagg ggggtagacg gatcctacaa
    17161 ctcggttatg aaggcagcag atattttgaa tgcaacaggc cttcctttcc agatcaacac
    17221 aaccgtaaca cgcttcaatg taaatgatct tttcgaaatt tataatctgg taaaattaat
    17281 aggcgcagtt gcatggcatg tttttcttct tgttcctgtt ggccggggta aagatctcaa
    17341 aggtaaagaa ttagatgcga tggcatatga ggatgtcctg aactggttgt acgatattga
    17401 gtccagaaat gagcttgaga tgaaggtaac ctgtgcaccc cattactacc gaattgtaaa
    17461 agaacggggg agtattccga agagcgctgg gtgccttgcc ggaaaaagct tcatgtttat
    17521 atcgcacaaa ggtattgctc aaccctgcgg ctaccttgaa atctcatcag gagatgtgcg
    17581 ggcccatggc gttaaaaaag tctgggaaga atctccagta ttcaatcagc tgagagacat
    17641 ctcttcgtac agaggaaaat gcggtggatg tcgctatctt aagatttgca gtggatgtcg
    17701 tgcccgtgcg tacgaactcc atggtgattt cctgcaggag gaaccttact gctcgtacgg
    17761 tcaccctgcg gaagagtaag cgattgatgt aaatcactca ccggaaggct catagttttc
    17821 aacagagcca gatgtgataa atacaaacgc atttgcttac cattgacaac tggatccatg
    17881 aacgaaagaa acctgacaca tgagagatga tgatggaggg aattgatcac accacatcat
    17941 ggactgcaca gataacatgt atgaaatgga caatatcagt ccttttcata atatggttgt
    18001 tgctaacccc ccaaccatcg tctggtgcgc ggccacttgt gacagatgat gcagatactg
    18061 cgggcaaggg aaaggcccag atagaacttg gtgttgagat attcacatgg caggatcggg
    18121 aaaggggaat aaagaccagg gaaacaggta cagaaatatc ttcaaccttt acctatggcg
    18181 tagcagatca tctcgatctg gtcgtgggga tcccttatgt ttggggtgct atggaaaaga
    18241 atggccagac cacatttgat gcggatcggc tctctgatat ctcacttgaa gttaaatggc
    18301 gcttctatga gaataaagtg tttcaagttg cttttaagcc ggggttaacc cttcctacag
    18361 gtaaccacag gaaagaattc ggaacaggca gaccaaccta tggtctcgtg tttattgcca
    18421 gtaaagagat gggagacatg ggctttcatt tcaacacctg ttacacgaga aatgaaaaca
    18481 aggttgataa acgagttaac ctctggtctg cttctgtggc agccacgtat gaggcgataa
    18541 aaggactgaa tttggtgggc aatgtgggac tcaaacataa tacagacccc gccattaaaa
    18601 cagctcctgc attctggctg gcgggcttta attattcttt caatgactac tttacaatag
    18661 atggagccct taaattccgt cttaacaggc aagaggctga ccgtgccatg atagctggaa
    18721 taaccatacg cttttgaggt aaaaaacagg ttccacggtt ttgttcaatt cactgtttca
    18781 cctgatggtt caattcgcca cacaaaaaag ttgcaagaat ttaatgaaat agcttgacat
    18841 gctttggcaa ttcatataca attgcgaatc gttatcattt aggggtcttt cctaatggtt
    18901 ccaacctatc gcgaaagata tcaggctatg gtgaaaaagc tgaaggacaa cagccataag
    18961 ctgacccctc aacgccttgc aatcatcaga attcttgcgg gaagtgagaa ccatccctct
    19021 gttgaagata tttataacct gttgcacaaa cgctttcccg gcatcagtca ggcaacagta
    19081 tatcgaaaca ttatgcttat taaatcactg ggtgaggttc tggaaatagg tttcgcagga
    19141 gcaagtactc gatatgacgg tataaaaccc ttccctcacc cccatgttgt ttgcctgaaa
    19201 tgtaataaaa taatagaccc ggaattggca agcctgcacg atatgacgaa agagatcacc
    19261 gacgagagcg gttttgaaat agtgacattc cgacttgact tcttcggtgt gtgcccggag
    19321 tgccgtgcca tgggctaatt ttttt
//
LOCUS       DAOOMC010000047        19171 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdus sp. isolate AMR_MDS_2685
            NODE_2906_length_19171_cov_5.947060, whole genome shotgun sequence.
ACCESSION   DAOOMC010000047 DAOOMC010000000
VERSION     DAOOMC010000047.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864760
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdus sp. (wastewater metagenome)
  ORGANISM  Syntrophorhabdus sp.
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae; Syntrophorhabdus.
REFERENCE   1  (bases 1 to 19171)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19171)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,729
            Genes (coding)                    :: 2,708
            CDSs (with protein)               :: 2,708
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 7 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19171
                     /organism="Syntrophorhabdus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2906_length_19171_cov_5.947060"
                     /isolate="AMR_MDS_2685"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2699756"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..375)
                     /locus_tag="PK125_06355"
     CDS             complement(<1..375)
                     /locus_tag="PK125_06355"
                     /inference="COORDINATES: protein motif:HMM:NF019498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HPB37763.1"
                     /translation="MLDVAREAYKGAVEEEACIFVKTNMRGIIMIVRNFNDPEVLQTT
                     YRAHYGATARMVLTSRFLQSMEFLAYAMLPAGNVIEEHVDEVEEIYLIAQGGGLMKVG
                     NEEKEVKEWDAIWIPAGEPHRLE"
     gene            complement(634..1218)
                     /gene="hisB"
                     /locus_tag="PK125_06360"
     CDS             complement(634..1218)
                     /gene="hisB"
                     /locus_tag="PK125_06360"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: protein motif:HMM:NF002111.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HPB37764.1"
                     /translation="MKRTGTVKRKTKETNISIKLSIDGEGYYEVSTGIPFFDHMLHLF
                     SKHGFFDLQIEAKGDIEVDYHHTVEDVGIALGKALTQALPSFDGIQRYGQAIVPMDES
                     LCLLAIDISGRPNLVWKTNMQGKIGTFDVEVVKEFFKGFVNESKCTLHVNILYGENLH
                     HKVEAIFKAFAKALRQALTRDERIKGALSTKGSL"
     gene            complement(1285..2154)
                     /gene="hisG"
                     /locus_tag="PK125_06365"
     CDS             complement(1285..2154)
                     /gene="hisG"
                     /locus_tag="PK125_06365"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: protein motif:HMM:NF013773.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HPB37765.1"
                     /translation="MKLKIGLPKGSLQETTFKLFKNAGYTIKLLERSYVPAIDDPELE
                     GLVIRAQEMARYVEDGILDMGITGYDWVLEQDAKVIELVRLRYGKVGFRGVKWVVAVP
                     SDSTIKTLEDLKGKKIATELVGYTKRFLKKKGIDATVEFSWGATEVKPPLLADAIIEV
                     TETGASLRANNLRIIDTILESETVLIANRSAWKDEWKRRKMESMTMLLKGALLAEEKV
                     GLKMNVPRTKLDKVTKILSSLHTPTISNLSDENWAAIEVIIDQKTVRDIIPNLRRAGA
                     QGIVEYPLNKVIP"
     gene            complement(2144..3430)
                     /gene="hisD"
                     /locus_tag="PK125_06370"
     CDS             complement(2144..3430)
                     /gene="hisD"
                     /locus_tag="PK125_06370"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF013013.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HPB37766.1"
                     /translation="MKVWDLEKEFDEFLVYAIGGREKKRQNTRSVVEKVRDRLLAKGE
                     EALIEFSTKWDGWPETLDLKLTQKEIISEASKISKRDLAIIRGMIKNVRDFHRKQGWR
                     KRTYKRKGLVVYEESVPVEKALVYVPGGTAPYPSSLIMGVVPAQLAGVSEVMVTTPAK
                     NGKINPYILAAANLLGLNDIYRMGGAQAIYAFSYGIGGIPKVDMIVGPGNAYVEEAKR
                     DVYGRVGIDMLAGPTELIILCTEPFSPEAVAWDLFSQTEHDEMATVGLFSPSREHLYN
                     ALKAIENHMASNPRREIIEKALKENSYFVFYREAKNAIKAINLIAPEHMELIGDEKVE
                     KDILYPGIIYLGPHTPVAMGDYYIGTNHILPTGGAGRFAAGLSVNTFTKRKAVVKIEK
                     GFLNRYGHKAIRLSEIEGLFAHGESIKVRKELANEA"
     gene            complement(3411..4685)
                     /gene="murA"
                     /locus_tag="PK125_06375"
     CDS             complement(3411..4685)
                     /gene="murA"
                     /locus_tag="PK125_06375"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: protein motif:HMM:NF012497.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HPB37767.1"
                     /translation="MDKFVIEGGERLAGTVRVGGSKNAVLPLLAATILQKGIYTIGNV
                     PRLRDVNTMIRLVTLLGVRAEWVDKNRLRIDTTGVDNHVAPYEVVKEMRASVLVLGSL
                     IGGMRKATVSYPGGCAIGERPINLHLQGLAALGCEVAIREGYVDVTANALKGGRVVFD
                     APTVGGTENILMAAVTAKGETVIENAAREPEVIDLVRMLKRMGAKIDGEGSDTITIKG
                     VRSLTPCDYDVIPDRIEAGTFLAACGITRGNIVIEGCVPHHLKAVTDKLREAGMDIIE
                     DGNSLRAAMTKKRPFAIDIKTTPYPGFPTDMQAQTMALMCISKGVSAITETIFENRMM
                     HAAELRRMGADVRVIGNTAIVTGKDMLSGAKVMATDLRASASLIIAGLAAYGTTEVSR
                     IYHIDRGYEAIEEKLKGLGAKISRRKDEGMGS"
     gene            complement(4670..5497)
                     /gene="prmC"
                     /locus_tag="PK125_06380"
     CDS             complement(4670..5497)
                     /gene="prmC"
                     /locus_tag="PK125_06380"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: protein motif:HMM:TIGR03534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HPB37768.1"
                     /translation="MRIRDIIEQHKKIEWLEAVNIIADALFMSKEGILVNLDQDVDEK
                     TCLHINDLLSKRERGMPLAYITKSKEFFSEKFYVDAGVLIPRPETELLVEEALKLITE
                     NKRINNLLDIGTGAGTIGLILAKKTQKHVVCTDISFDAIRIAMRNAENLGVRGLIHFV
                     CADLFSGICGSKFDMILANLPYVGSGEMDSLMADVKNYEPRLALDGGAGGIQIYRRLI
                     EEVPHHLKDNGYVLCEVGGYVQANKISEMLRALGMNTAVKKDLSGHDRVLIGSWTSS"
     gene            complement(5505..6569)
                     /gene="prfA"
                     /locus_tag="PK125_06385"
     CDS             complement(5505..6569)
                     /gene="prfA"
                     /locus_tag="PK125_06385"
                     /inference="COORDINATES: protein motif:HMM:NF001859.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HPB37769.1"
                     /translation="MFERLTEIEAKYNEIEDRMSKQETIADFDLYKKLAKERTELKDI
                     VDLYREWKKKQEEIDKAFELVESDQDDEMRALARDEIAILTQDKEEIEIQLKEKLLNK
                     QARHANSMFLEIRAGTGGEEAALFAHDLLTMYMKYAEKMRWKTELMSASTSDLGGLKE
                     VVLVVEAKDAYNKLKYESGVHRVQRVPVTEAQGRIHTSAVTVAVLPEPEERELHINPE
                     DLRIDVFRSSGPGGQHVNTTDSAVRITHIPTGLVVTCQDEKSQHKNKAKAIRVLRARL
                     QEKMESEQEHEISEERKKQVGTGDRSERIRTYNFPQGRVSDHRIGLTLYKLQEVLNGD
                     LEDIVNPLIAHFQAEALKKE"
     gene            complement(6569..6775)
                     /gene="rpmE"
                     /locus_tag="PK125_06390"
     CDS             complement(6569..6775)
                     /gene="rpmE"
                     /locus_tag="PK125_06390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014697411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HPB37770.1"
                     /translation="MKKGIHPELKKTVVKCACGSTFETLSVREKISVEICAKCHPIFT
                     GKEKRIDSAGQVEKFEKRYGKKTK"
     gene            complement(6777..8030)
                     /gene="rho"
                     /locus_tag="PK125_06395"
     CDS             complement(6777..8030)
                     /gene="rho"
                     /locus_tag="PK125_06395"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017631298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HPB37771.1"
                     /translation="MHLKDLKRKRIGELTQIAKEMNIENAAGLRKQELIFSILQALVD
                     KNESVYGEGILEILSEGFGFLRSTDSNYLPGPDDIYISPSQIKRFGLKTGDTISGQIR
                     PPKDNEKYFALLKVETINYDDPTIAKDKIIFDNLTPIYPNERIQLETIPENMSTRVMD
                     LFTPIGKGQRGLIVAPPRTGKTMLLQAIANSITSNHKEIQLIVLLIDERPEEVTDMMR
                     SVNGEVISSTFDEPATRHVQVAEIVIEKAKRLVEHKRDVVILLDSITRLARAYNTVVP
                     SSGKILSGGIDASAMQKPRRFFGAAKNIEEGGSLTIVATALIDTGSRMDEVIFEEFKG
                     TGNMEIYLDRKLAEKRVFPAIDINKSGTRKEELLLENGDLSRIWLLRKVLQPMNPVES
                     MEFLLEKMADTESNKDFLSSMSRGG"
     gene            complement(8030..8629)
                     /gene="coaE"
                     /locus_tag="PK125_06400"
     CDS             complement(8030..8629)
                     /gene="coaE"
                     /locus_tag="PK125_06400"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: protein motif:HMM:TIGR00152.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HPB37772.1"
                     /translation="MILVGITGIIGSGKTTVSNMLSQEGIPVIDLDRLGKEITDYDEV
                     IRDIEEVFGTEYIANKKVDIVKLRTIAFVDVKTRKKLEAIIHPRVRAELWKKIEHYRD
                     MGARVVVVDAPLLYETGMYKSMDKVVVVSADMDKIRERLKLRGMDEEDTDRRLPHQIP
                     LADKEKMADYILYNNGLEEDLRKELKTLLLRIKEWEVRG"
     gene            complement(8626..10986)
                     /gene="gyrB"
                     /locus_tag="PK125_06405"
     CDS             complement(8626..10986)
                     /gene="gyrB"
                     /locus_tag="PK125_06405"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF011501.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HPB37773.1"
                     /translation="MKEYGAESIKILGGLDAVRKVPAMYIGNTAQEGLHHLVYELVDN
                     SVDEALEGYCSRVMITLHRDNSVTCQDNGRGIPVAMHKEENMSALEVVLTKLHAGGKF
                     DKDSYLYSAGLHGVGLSVVNALSEFLEIEVRRDGKVYFQRYEGGNKITELKVIGDTEK
                     TGTKVHFKPDAAIFESIEFSYETIAHRMREISFLNNGIHIIVTDEKKGRKHEFKHEGG
                     IKAFVQYLNTNKTVLFSEPIHVISTKQPLDYIEVAIQYNDGYNENIYSFVNNVNTQEG
                     GTHVAGFRSALTRSINNFIATNMSQKSKENISGDDLREGLVAVISIKIQNPQFEGQTK
                     AKLGNSEIKGLVESVLNDKLTEYLELNPDVAKAIINKALEAKRAREAAKKAKELIKNK
                     SLLESGILPGKLADCQESDPNLCELFIVEGDSAGGSAKQGRNRKIQAILPLRGKILNV
                     EKSRQEKILTNQEIKNVYLALGINSDNIHRLRYNKVIIMTDADVDGSHIRTLLLTLFY
                     RKMPELITNNHLFIAQPPLYKIKHGSKETYAKDEEEFERFITKRGMEKISCYVEGSLI
                     DAARLKEIVEKMKSMELFFREMEANGISQIIMFKLFQNDIYTREDFEAPDKLNTIKEE
                     LESAGHSVELIKDREHNLFSIQISDDTKKEQAITVDYELCCQGDYKQYIKIYKEISPF
                     YNQSIKVVEGNKEFYPLNTEDFLRIINERGKEGISIQRYKGLGEMNPEQLWATTMDPE
                     RRSIIKVSIEDAIEADQVFTVLMGSNIETRRLFIEENALTVRNLDI"
     gene            complement(10988..12073)
                     /gene="dnaN"
                     /locus_tag="PK125_06410"
     CDS             complement(10988..12073)
                     /gene="dnaN"
                     /locus_tag="PK125_06410"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HPB37774.1"
                     /translation="MNIIIDKNSILLPISKIVGITEKRSLMPILSNIMIDFKKTDTTI
                     YSTDLELSAIGQIDFKAPEERKIVIHGRKFLEILREMDTGEIILQVDENILTIKQKQT
                     EIVLSLQDSEEFPEVKKIEGNEEYVINGAALLEMIEKVSFAISVDETRYVLTGMHISS
                     RDGNIMVVGTDGFRMAFYQKQIEGMKNFKGITIPKRSLFEIEKIIDGDENVLMILEEK
                     HVQISTKNILVISRVIEGSFPDYENVIPQTNENIARIDKESFMKGLKKVSSIIGRSEP
                     IKMNLTEGKLEMESESDIGRAKEILDIDYKGEDITLNFNVRFVMDVVSHIESNIVVMS
                     VPTTYGAVLFKEENNENYKNIVMPIRI"
     gene            complement(12192..13508)
                     /gene="dnaA"
                     /locus_tag="PK125_06415"
     CDS             complement(12192..13508)
                     /gene="dnaA"
                     /locus_tag="PK125_06415"
                     /inference="COORDINATES: protein motif:HMM:TIGR00362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HPB37775.1"
                     /translation="MVDILAQIKPKISKIISEESYKTWIEPIEFVEYVDNKCVLMVPN
                     TFFKDWVIQHFESILVTLMREITSTNNRIEYVIKKEQRTDGKRGVVLRRPSQFNVFNP
                     KYTFENFVVGSSNQFANAACLAVATNPGKTYNPLFIYSGVGLGKTHLLNAIGNFLVKH
                     GKSNPEKICYITAEHFTNELINALRYEKMEDFRNRFRKMDVLLIDDIQFIAGKERTQA
                     EFFHTFNALYDNMKQVVLTSDKFPAEIENIEERLRSRFDWGLIADIQPPDIETKVAIL
                     NKKAESDHIALPLDVAFFIASHTEDNIRSLEGALIRIGAFSSLNNSPVTIDLVKEVLG
                     HMIKTKKKEITIDLIIKEVSSFFNLKPSEIRSSRRIKSIILPRQVAIYLSRKLTDFSL
                     VSIGEKFGGKDHATVLHSIKKVEEEMKIKKEFKNTVERIETRIKST"
     gene            13759..14211
                     /locus_tag="PK125_06420"
     CDS             13759..14211
                     /locus_tag="PK125_06420"
                     /inference="COORDINATES: protein motif:HMM:NF015168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HPB37776.1"
                     /translation="MSIIAHADEWDFALRRAKKEDKPTILYFFTPYCFYCQTMDKEVL
                     ANKEVFAILNKNVVYLRIDVEKREDLARFYGVRGYPTTVFLQPNGQQIIQIPGYINRK
                     DFKKLLTFVTGKHYKTMKLREFLQKTACLEPLKNRPEPQQIFHPIWKG"
     gene            complement(14158..14652)
                     /locus_tag="PK125_06425"
     CDS             complement(14158..14652)
                     /locus_tag="PK125_06425"
                     /inference="COORDINATES: protein motif:HMM:NF013073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HPB37777.1"
                     /translation="MNTKDTQIVLFEKQKRFRTVVFLLCFVSVVFSCVPIKVRTVAPP
                     SGLRAHVVSTSIDLLGKTYRAGGRGPDYFDCSGLVSYVYKKVGLSLAPTAEEQGRSGI
                     EVSLDSVLPGDLVVFRIKGDFHVGIMVNEREFIHASKSRGVAVDNLTLPYWMKNLLGF
                     RSVL"
     gene            complement(14618..15280)
                     /locus_tag="PK125_06430"
     CDS             complement(14618..15280)
                     /locus_tag="PK125_06430"
                     /inference="COORDINATES: protein motif:HMM:TIGR01491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IB family phosphatase"
                     /protein_id="HPB37778.1"
                     /translation="MTIKVAFFDCDGTLTKARNSWEYLHRKLNIWDKQAERYQTLFRE
                     GKISYYEFCKNDALLWKGLPVSSVVSLLKDITYHSGAYEVVKALKKRGILTVILSTGL
                     SLLVERVREDLGIDMSFSNELLSENGHLTGEIKINVGFNKKGYLVKKILGDIGVSRKE
                     ACAVGDGDGDTSMFEEVVLAIGFRPQDHVSSVIHRAICGRSLLEMIEIIDEYERHTNS
                     VV"
     gene            15359..15925
                     /locus_tag="PK125_06435"
     CDS             15359..15925
                     /locus_tag="PK125_06435"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015786.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HPB37779.1"
                     /translation="MKPVDWDKRYKEGFYRQTKEAHPLLQQFWHKIPKGNVIDIAMGS
                     GRNALFLSERGYSVCGLDRSSEALKIARQATVEKNRSIFLIRGDAGSLPFKTSSASGV
                     IIFYFLLRDIMEEIIGLLKTGGVLVYETFLKRQNTIDQYRNPDFLLEDGELLSYFKNL
                     ELLFYEETILTIDGKQKATARFVGRKRC"
     gene            15919..16533
                     /locus_tag="PK125_06440"
     CDS             15919..16533
                     /locus_tag="PK125_06440"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: protein motif:HMM:NF013468.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HPB37780.1"
                     /translation="MLIEWDPTRPKKKATDLIRKTLEEGGIVGYPTDTLYGMGCDLFN
                     IKAIRKLHQIRRLPTRWALSIICRDLKEVSEYAVMSDYAFGALKECLPGPYTFVLKAK
                     KILPKLLMTERKEVGVRISSHPVPTALTRLIERPILNTSARVSGQEPFTDPRDIEKAF
                     KGGIDLVIDGGIIPAQPSTVVRLNDDNIEVLREGKGSLKNLLMR"
     gene            complement(16511..16852)
                     /locus_tag="PK125_06445"
     CDS             complement(16511..16852)
                     /locus_tag="PK125_06445"
                     /inference="COORDINATES: protein motif:HMM:TIGR02605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HPB37781.1"
                     /translation="MPIYEYRCKKCKKLSSFLLLRITEEIDPHCKHCGNKDVIRVISR
                     VTVLKSEEQRLESMLDPSKFSDLDENDPRSVERVMRRMGKEFGDELGEDFEESMEEAL
                     SQENAISSEDS"
     gene            complement(17374..17673)
                     /locus_tag="PK125_06450"
     CDS             complement(17374..17673)
                     /locus_tag="PK125_06450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB37782.1"
                     /translation="MDKAKEEKLLGHVGNLYLGLNYYHRAISFYKNNWRGLVKLEIRK
                     ARVVFLGNIGIVYIERIEFCRTIIVFYKKQLDTARLIPDEQADGRALGNKGPPML"
     gene            18145..18276
                     /locus_tag="PK125_06455"
     CDS             18145..18276
                     /locus_tag="PK125_06455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB37783.1"
                     /translation="MADWIAILIHSAQGELLDYCGRTIDEERARNKGKYKQTTGFAK"
     gene            18652..18801
                     /locus_tag="PK125_06460"
     CDS             18652..18801
                     /locus_tag="PK125_06460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB37784.1"
                     /translation="MASMETNDFPVETISALANIWADWLVGFHLAVAQITKTIIDAGE
                     ENIDE"
     gene            18869..19084
                     /locus_tag="PK125_06465"
     CDS             18869..19084
                     /locus_tag="PK125_06465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB37785.1"
                     /translation="MFMVIIKWKDGTSSSMNLSIQHAPANPTEEDFKDLLTMTKAVVD
                     TALNDGSFPGRSKKDLDTIEIITTSAN"
BASE COUNT         5013 a         4439 c         3581 g         6138 t
ORIGIN      
        1 ctccagtcgg tgcggttcgc cggcaggtat ccagatagca tcccactcct ttacctcttt
       61 ttcttcgtta cccactttca ttaaaccgcc gccttgcgca atgagatata tttcttcgac
      121 ctcatccaca tgctcctcta ttacattgcc agcaggaagc atggcataag caaggaattc
      181 catgctttgt agaaaccgac tggtgagaac catccgtgcg gtggcaccgt agtgcgctct
      241 gtatgtagtt tgaaggactt ctggatcgtt gaaatttctt actatcataa tgattcctcg
      301 catatttgtt ttgacaaaga tacacgcctc ttcttcgact gcccctttgt atgcctcgcg
      361 agctacgtca agcaacggga agggacacct gtttctgtgc tcctcgaggc tcctttttca
      421 acagcattag ggttttgtgc agaaaccgta aaccagaact ttggttcttt tccgggcgaa
      481 actaacatgc aaacacattt cccatgcttt gcagattacc catgctatag taaatggcag
      541 ttatcatact gatgccgctc aaaatcctgt tatttcagtc tgtatcgttt cgcactgtta
      601 aatcaaatga tcctgtatca aaccgcctta aacctaaagg cttccctttg ttgaaagggc
      661 gcccttgatt ctctcgtccc tggttagggc ctggcgtaag gctttcgcaa atgccttgaa
      721 gattgcttct actttatgat gcaaattctc accatatagt atgtttacat gcaatgtgca
      781 cttggattca ttcacaaaac ccttaaaaaa ctccttaacc acctcaacgt caaaggttcc
      841 gattttgccc tgcatgtttg ttttccacac caggttcggt cttccactaa tatctattgc
      901 taaaaggcaa agggattcat ccatgggaac aatagcctga ccgtagcgct gaatcccgtc
      961 aaaactcgga agagcctgcg ttaaggcctt tcccagagca ataccaacat cttccacagt
     1021 gtggtgataa tcaacctcaa tatcgccctt tgcttctatc tggaggtcaa aaaagccgtg
     1081 ttttgaaaac aggtggagca tgtggtcaaa aaaaggtatt cccgtggaaa cctcgtagta
     1141 tccttcccca tcaatggaaa gcttgatcga tatgttggtc tcctttgttt ttcttttcac
     1201 tgttccagtt cttttcacgt ccacccccta tctgatttgc attttttcac acacaactgt
     1261 tcccggctaa ctgttcagcg gttttcaagg tatcaccttg ttcagtggat actcaactat
     1321 tccttgggcg cctgcccgtc tcagattcgg aataatatcc ctaactgttt tctgatcaat
     1381 aattacctct atggctgccc agttctcatc agaaagattt gagatggtgg gcgtgtgcaa
     1441 agaggaaagt atttttgtaa ctttatccag tttggtcctt ggaacattca tcttgaggcc
     1501 caccttctcc tcagcaagga gcgctccttt tagaagcatg gtcatgctct ccatcttccg
     1561 tcgcttccat tcatccttcc atgcgcttcg atttgctatg agcactgttt cggattcaag
     1621 gattgtgtct atgattctca gattgttggc cctcagtgat gctcctgttt cagtaacctc
     1681 aataattgcg tctgcaagaa ggggtggctt tacctctgtt gctccccatg aaaactcaac
     1741 ggtggcatcg ataccctttt ttttaagaaa cctttttgta tatcctacaa gttctgttgc
     1801 tattttcttt cccttcaggt cttccaaggt tttgattgtc gaatcactgg gcacggcaac
     1861 aacccacttc acccctcgaa atccgacctt accatatctt agtcgcacta gttcaatcac
     1921 ttttgcgtcc tgttccagaa cccaatcata gccggtgatg cccatatcga ggattccatc
     1981 ctcaacatac cgtgccatct cctgcgcccg aataacaagc ccttcaagct ccgggtcatc
     2041 gattgccgga acatacgacc gctcgagaag ctttattgta tatccggcgt ttttgaaaag
     2101 cttgaatgtg gtctcttgca agctcccttt gggaagccct attttaagct tcatttgcta
     2161 gctcctttct tacctttata gattctccgt gtgcaaaaag accttctatt tccgaaagcc
     2221 ttatcgcttt atgaccatac ctgttaagaa atcctttttc tattttaaca accgcttttc
     2281 gcttcgtaaa cgtgttgaca gagagtcctg cagcaaatct tccagctcct ccggtaggaa
     2341 gtatgtggtt tgtaccgata tagtaatcac ccatagccac cggtgtgtgg ggacccaaat
     2401 agatgatacc gggataaaga atatcttttt ccactttctc atcaccgata agctccatgt
     2461 gctctggagc aatgaggttg atagccttta ttgcattttt tgcttcgcga taaaaaacaa
     2521 aatagctgtt ctcttttagc gccttttcaa taatctctct tcgcgggttt gatgccatat
     2581 ggttttcaat ggcctttagc gcgttataga ggtgttccct ggaaggggaa aaaagcccaa
     2641 ccgttgccat ctcatcgtgt tctgtctgcg aaaaaagatc ccaggccacc gcctccggtg
     2701 agaacggttc agtacacagg atgattaatt cggtgggtcc tgccaacatg tcaattccta
     2761 cgcgcccata cacgtctctt ttcgcctctt caacgtatgc gttccccggt ccaactatca
     2821 tgtcgacctt tggtatgcct ccgatcccat acgaaaaagc gtagatggcc tgagcacccc
     2881 ccattctgta gatatcattg agtccgagaa ggttcgctgc cgcaagtata tacggattga
     2941 tctttccgtt tttggccggg gttgtaacca tcacctcgct aacccctgct aactgagccg
     3001 gaacaacacc cattattaaa gatgaagggt agggcgctgt tcctcctggg acataaacaa
     3061 gagctttttc taccggcacg gattcttcat agacaacgag gcccttcctt ttgtatgttc
     3121 gttttctcca gccctgcttc ctgtggaagt ctcggacgtt ttttatcatg cctcggataa
     3181 tggcaagatc tctcttgctt attttggatg cttcggatat tatttccttt tgtgtaagtt
     3241 taaggtccaa agtttctggc catccatccc atttggttga aaattctatc agtgcttctt
     3301 cgccttttgc taaaagacga tcacgaacct tctcaacaac cgaccgggta ttttgtcgtt
     3361 ttttctccct gcctccgatc gcataaacga gaaattcgtc gaactctttt tcaagatccc
     3421 ataccttcat ccttcctcct tgagatcttt gctccaagac ccttcagctt ttcttctatt
     3481 gcctcatatc ctctgtcgat atgataaatt ctggagacct ctgtggtgcc gtaggcagca
     3541 agccccgcta ttattaggga ggcacttgct cgcaaatcag tagccataac ctttgctcct
     3601 gacagcatat ccttacctgt tacgatggcg gtgttgccta tcacccgtac atcggcaccc
     3661 attcttctta attctgctgc atgcatcatc ctgttttcaa atatagtctc tgttatagca
     3721 ctcactcctt ttgaaataca catgagagcc atggtctgag cctgcatgtc tgttgggaat
     3781 ccgggatatg gggtggtctt tatgtctatt gcaaagggtc gttttttcgt cattgcagcc
     3841 cgcagactat taccgtcttc aataatatcc attcctgcct ccctcagctt atctgtaaca
     3901 gccttgaggt ggtgaggaac acacccttca ataacaatgt tgccccttgt aataccacat
     3961 gcagctagaa aggttcccgc ctctatccta tcaggaataa catcatagtc acaaggcgtt
     4021 aaagagcgca cccccttgat ggtgatagta tcactgcctt ctccatcaat ttttgccccc
     4081 atacgcttta acatacgcac aagatctatc acttctggct cacgggctgc gttttctatc
     4141 actgtctcgc cttttgcagt aacagctgcc ataagaatat tttctgtgcc gcctacggtg
     4201 ggcgcgtcaa agacaactct tccgcccttc aatgcatttg ccgtcacatc aacgtacccc
     4261 tctcggattg ccacctcaca cccaagggct gcaagtccct gaagatgaag attaatgggt
     4321 ctttcgccta tggcacatcc accagggtac gaaaccgttg cttttctcat gccgccgata
     4381 agggagccaa ggaccagaac agaggcgcgc atttctttaa caacttcata tggcgcaaca
     4441 tgattgtcga cccctgttgt gtcaatcctc agcctgttct tgtcaaccca ttccgccctg
     4501 acaccaagaa gggtgacaag tcttatcata gtgttaacgt ccctaagccg gggaacgttg
     4561 ccaatggtat agataccctt ttgtaagatt gttgccgcaa ggagtggcaa caccgcgttt
     4621 tttgatccgc cgaccctcac tgttccggcg aggcgttctc cgccttctat tacgaacttg
     4681 tccatgatcc tattagcacc ctatcatgac cagaaaggtc tttttttaca gcagtgttca
     4741 tccccaaggc tcgaagcatt tcgcttattt tgttcgcctg tacataacct cccacctcgc
     4801 acagcacgta accattgtcc tttaggtggt gaggcacctc ttcaataagt cttctatata
     4861 tttgaatgcc tccggcacct ccatcgagcg ccagtcttgg ttcatagttt tttacgtctg
     4921 ccatgagact gtccatttcc ccagaaccga catacggcag atttgccagt atcatatcaa
     4981 actttgagcc acagatacca ctaaagagat ctgcacacac aaaatgtatg agcccccgca
     5041 caccaagatt ttctgcgttc ctcatggcaa tgcgaatagc gtcaaaggaa atatccgtac
     5101 aaacaacgtg tttctgtgtc ttttttgcca gaataaggcc gattgtgcca gcaccagttc
     5161 ctatgtccag aagattgttt atcctcttgt tttcggtgat gagtttaagt gcctcttcca
     5221 caagaagttc ggtttctggc cttggaataa gaaccccggc gtcgacataa aatttttctg
     5281 aaaaaaattc cttacttttc gttatgtatg ccaagggcat tcctctctct ctttttgaga
     5341 gaaggtcatt gatgtggaga caagtctttt cgtcaacatc ctggtcgaga ttaactaaaa
     5401 tcccttcttt gctcatgaaa agagcgtcgg cgattatatt caccgcttca agccactcta
     5461 tttttttgtg ttgttctata atatcccgaa tgcgcaaaag aggtttactc tttttttaag
     5521 gcttctgcct gaaaatgggc aatcagcggg ttaacaatgt cttctaggtc accattcagg
     5581 acttcttgaa gtttgtagag ggtaaggccg attctgtgat cgcttactct tccctgaggg
     5641 aagttatagg tccttatacg ctcgctacgg tcgcccgtgc cgacctgttt ttttcgttct
     5701 tctgatattt catgttcttg ttcgctttcc attttttcct gaaggcgagc ccttaaaacc
     5761 cttattgctt tggctttgtt tttatgttgt gatttctcgt cctggcacgt cacaacaagg
     5821 ccagttggta tgtgggtaat tctcactgca gaatcggtgg tgttcacgtg ttgccctccc
     5881 ggaccgcttg atctgaacac atctattcgt agatcttcgg ggttaatgtg aagctccctt
     5941 tcttctggtt cggggagaac cgcaacggtc acggcagatg tgtgtattct cccctgcgcc
     6001 tctgttacgg gtactctctg cacacgatgg actccgcttt catattttaa cttgttgtat
     6061 gcgtcctttg cctcaacaac gagaacaacc tctttgagtc cccctaggtc tgaagtgctt
     6121 gcactcatga gttctgtttt ccaacgcatc ttctcagcat atttcatata catggtcaaa
     6181 aggtcgtggg cgaacagcgc cgcttcttca ccgcctgttc ccgctcttat ttcgagaaac
     6241 atgctgtttg catgtcgcgc ttgcttattg agcagttttt cttttagttg aatctcgatc
     6301 tcttccttgt cttgggttaa aattgcaatc tcgtctcgtg caagcgccct catttcatcg
     6361 tcctgatcag attcaactaa ctcaaaggcc ttgtctatct cttcctgttt cttcttccac
     6421 tcacgataaa gatctacaat atccttcagt tcagtgcgtt ctttcgcaag ttttttatac
     6481 aggtcaaaat cagcaatggt ctcctgtttt gacatcctgt cttcgatctc attatattta
     6541 gcttcgatct cggttaaacg ttcaaacatt atttggtctt cttaccatac cgtttttcga
     6601 acttttctac ctgtcctgcc gaatcaatcc ttttttcttt tcctgtgaag atggggtggc
     6661 attttgcaca aatttcaaca ctgatttttt ctctcacaga caatgtctca aaggtgcttc
     6721 cgcacgcaca cttaactact gtctttttta attctggatg tatacctttt ttcatgttaa
     6781 cctcctctgc tcattgagct aagaaaatct ttattcgact cggtgtcagc catcttttcg
     6841 agcaaaaact ccatggattc taccggattc attggctgaa gcacttttcg taaaagccat
     6901 attcgagaaa ggtcgccatt ttcaagaagc aactcttcct ttcttgttcc agatttgttt
     6961 atatcaattg caggaaacac tcttttttca gccagttttc tgtccaggta tatttccata
     7021 ttgccggttc ctttgaattc ttcaaagata acctcatcca tcctgctccc ggtgtctatc
     7081 aaggctgttg caacaatggt aaggctgcca ccttcttcaa tattttttgc tgcaccaaaa
     7141 aatcgtctcg gcttctgcat tgctgacgca tctatccctc cggaaagtat ttttccgctt
     7201 gaaggcacta ctgtattata ggctctggca agcctcgtta tgctgtcaag gagtataacc
     7261 acatctcttt tgtgttcaac aagacgcttt gccttttcta taactatttc agccacctga
     7321 acatgtctcg tcgcgggctc atcaaaggta gagcttataa cctctccatt aacagaccgc
     7381 atcatatctg tcacctcttc aggtctttcg tcgataagaa gaacaatgag ctgaatctcc
     7441 ttgtggttcg atgtaatgct gtttgcaatg gcctggagaa gcattgtttt ccctgtcctc
     7501 ggcggtgcga cgatcagacc tctctgcccc ttaccaatgg gtgtaaaaag atccatcacc
     7561 cttgttgaca tgttctcggg tattgtctcg agttgaatcc gctcgttcgg atatatgggt
     7621 gtaaggttgt caaagataat tttgtctttt gctatggttg ggtcatcata gtttatagtc
     7681 tccaccttca ggagggcaaa atacttctcg ttatctttgg gtggtcttat ttgtcctgat
     7741 atggtgtcgc ctgttttcag cccaaaacgt tttatctggg agggagaaat atagatatcg
     7801 tccggtcctg gcagataatt cgagtcggtt gaccgcaaaa aaccaaaccc ttctgaaagg
     7861 atttccagaa tgccttcgcc gtagacggac tcgttcttat caacaagggc ctggagtata
     7921 gaaaaaatga gttcctgctt tcttaatcca gctgcatttt ctatattcat ttcctttgca
     7981 atttgcgtta gctcgccaat cctttttctt ttcaagtcct ttaggtgcat taacctctta
     8041 cctcccattc ttttatcctt aacagcaggg ttttgagctc tttcctgagg tcttcttcca
     8101 ggccattatt atacaaaata tagtcggcca ttttttcttt gtcagctagg gggatctgat
     8161 ggggcaatct tcggtcagta tcctcttcgt ccattccacg caacttcaat cgctctctga
     8221 ttttatccat gtctgctgaa acaacaacca ctttgtccat gctcttgtac ataccggttt
     8281 cgtataaaag aggtgcgtca acaactacta cgcgggcacc catgtctcta tagtgttcta
     8341 tttttttcca aagctctgct cttacccttg ggtgtatgat tgcttccagc tttttccgtg
     8401 ttttcacatc aacaaaggct atcgtcctca actttacaat atctaccttc ttgttcgcta
     8461 tatattcagt accaaaaact tcttctatat ccctgatgac ctcatcgtaa tcggttattt
     8521 ctttccctag tctgtcaaga tcaatgacag ggataccttc ttgtgacagc atgtttgata
     8581 cggttgtttt gccgctacct ataatgcccg ttattccaac tagaatcata tatcaaggtt
     8641 tcttacagtc agggcatttt cctcgataaa gagcctccgc gtctcgatgt tacttcccat
     8701 gagaacggta aacacttgat cagcttctat ggcatcctcg atcgatactt ttattatgct
     8761 tcttctttct gggtccattg tggtggccca caactgttcg gggttcatct ctccaaggcc
     8821 tttatatctc tgtattgaga tgccttcttt tccccgctca ttgatgatgc gaaggaaatc
     8881 ttcggtattt aatggataga attctttatt accttctaca actttgatgc tctggttgta
     8941 gaatggactt atctctttgt atattttgat atattgttta taatcaccct ggcaacaaag
     9001 ttcgtaatca acggtgatcg cttgttcttt ttttgtatca tcactaattt gtatggagaa
     9061 aaggttgtgt tccctgtctt tgattaattc tacagaatgg cctgcacttt ctagttcttc
     9121 ctttatggta ttgagcttgt caggtgcttc aaaatcttct ctcgtatata tatcgttctg
     9181 gaataattta aacatgatta tctgtgaaat tccgttggct tccatttctc tgaaaaatag
     9241 ttccatactt ttcatttttt cgacaatttc tttcagtctc gcagcatcta taagagagcc
     9301 ctccacataa caacttatct tttccatccc tcgttttgta ataaacctct caaattcctc
     9361 ctcgtctttt gcatatgttt ctttgctccc atgtttaatc ttgtaaaggg gcggttgtgc
     9421 aataaaaaga tggttgttgg ttatcaactc aggcattttt ctgtaaaaaa gggttaaaag
     9481 aagcgttcgt atatgggaac catctacatc agcatcggtc ataataataa ccttattata
     9541 tctaaggcgg tgaatattgt cagaatttat acccagggcc agatatacat tcttaatctc
     9601 ttggtttgtc agtatttttt cttgcctgga tttttcgaca ttaagtattt ttcccctaag
     9661 tggaagtatt gcttgtattt tgcggtttct tccctgtttt gctgaaccac cagcagagtc
     9721 tccctctaca ataaaaagtt cacagaggtt agggtcactt tcctggcagt cggccaattt
     9781 tccaggcaat attccacttt caaggagact tttatttttt atcagttcct ttgctttttt
     9841 tgcggcttct ctcgctcttt tcgcttcaag ggctttattt ataattgctt tggctacatc
     9901 aggatttaat tctaaatact cggttagttt gtcgttgaga acagattcta caaggccttt
     9961 aatttcactg tttcccagtt tagctttggt ctgtccttca aattgtgggt tctgtatttt
    10021 tatactgatg actgccacaa gaccttccct taaatcatcc ccggatatat tttccttgga
    10081 tttctgggac atgtttgttg caataaaatt atttatactt ctcgtaaggg cacttctaaa
    10141 accggcgacg tgtgtaccgc cttcttgtgt gtttacatta ttcacaaaac tgtaaatatt
    10201 ttcattatat ccgtcattat actgaatggc aacctctatg taatcaaggg gttgtttcgt
    10261 acttatgaca tggattggct cagagaaaag cacagttttg ttcgtattca aatattgtac
    10321 aaaagctttt attccaccct cgtgtttaaa ttcatgtttc cttcctttct tttcatccgt
    10381 aacaattata tgtattccgt tattaagaaa ggatatttcc ctcattctgt gagctattgt
    10441 ttcataacta aattctattg attcaaaaat agctgcatct ggtttaaaat gtaccttggt
    10501 tcctgttttt tccgtatccc ctataacttt caattctgta attttattgc cgccttcata
    10561 tctctgaaag taaacctttc catctcttct tacctcaatt tcaagaaatt ctgaaagggc
    10621 attaactact gataagccta ctccatgtaa acctgccgaa tataaatagc tgtctttatc
    10681 aaacttgcca cctgcgtgga gctttgtaag aaccacctca agggctgaca tattttcttc
    10741 tttatgcatt gcaacaggaa tacctcgccc attatcttgg catgttacac tgttgtctct
    10801 gtggagtgtt atcatcactc tactgcaata tccctccagt gcttcatcaa cactattatc
    10861 aactaattca taaaccagat ggtgaagacc ttcctgcgct gtgtttccta tatacatggc
    10921 tggaacctta cgaacagcat caagcccacc caatattttt atactttctg ctccgtattc
    10981 tttcatttta tatcctgata ggcattacaa tatttttata gttttcatta ttttcttctt
    11041 taaaaagaac tgcaccatat gttgttggca cactcattac aacaatattg ctttcaatgt
    11101 gagatacaac atccatcaca aatcgaacat taaaattgag agttatatcc tcccctttat
    11161 aatcaatgtc taatatttct tttgccctcc caatatcaga ttctgattcc atctctaatt
    11221 ttccttctgt aagattcatc ttgataggtt ctgatcgccc tataattgag gaaacctttt
    11281 ttaaaccttt cataaatgat tctttatcaa tccttgcaat attctcgttt gtttgtggaa
    11341 ttacattctc gtagtctgga aaacttccct ctataactct tgatataacc aaaatatttt
    11401 ttgtacttat ctgaacatgt ttttcttcta atatcatgag tacattttca tctccatcta
    11461 taatcttttc aatttcaaag agagatcttt ttggtatggt gattccctta aaatttttca
    11521 ttccctctat ctgtttttga tagaatgcca ttctaaatcc atcagtacct accaccataa
    11581 tattcccatc tctactgctt atatgcatac ccgttaaaac atatctcgtt tcatcaactg
    11641 aaatcgcaaa actaactttt tcaatcatct caagcagtgc tgccccgttt ataacatact
    11701 cttcgtttcc ttctatcttt tttacctctg gaaactcctc agaatcctgg aggctcagca
    11761 caatttcagt ctgtttctgt ttaattgtca gaatattttc atcaacctgt aatattattt
    11821 ctccagtatc catttctctt aaaatttcga gaaattttct tccatgaata acaattttcc
    11881 tttcttctgg agctttaaaa tcaatttgcc ctatagcact caattctagg tcagttgaat
    11941 agattgttgt atctgttttc ttgaaatcaa tcataatatt agaaagaata ggcattaatg
    12001 aacgtttttc agtaattcca acaatttttg aaataggtaa aagaatagaa tttttatcta
    12061 ttattatatt cataaatacc tctcttagtc gtaataaggg ctgttaatat gttgaaaact
    12121 cgtaattttc ttgaaataac aagaaaaaaa tgcaattcct attgtgaaaa aacagaaata
    12181 tttttgtaga ttcatgtgga ttttatcctt gtttctattc tttcaacagt atttttaaac
    12241 tctttcttaa ttttcatctc ttcttcaact tttttaatag agtgtaatac tgtagcatgg
    12301 tcttttcctc caaatttctc tccaatacta accagtgaaa aatccgttaa ttttctagat
    12361 aaatatattg caacctgcct tggtagtata attgatttta ttcttcgtga agaccgtatt
    12421 tctgagggct tcaaattaaa gaaagaggac acttccttaa tgataagatc aatcgtaatc
    12481 tcttttttct ttgttttaat catatgacct aatacttctt taacaagatc tattgtaact
    12541 ggactgttgt ttaaagatga aaatgcccca atacgaatta gagcaccttc gagggaacgg
    12601 atattgtctt cagtgtgtga agcaataaag aaagcaacat cgagaggtaa tgcaatatgg
    12661 tcactctctg cttttttatt caatattgca acctttgttt caatgtcagg tggctgaata
    12721 tctgcaatga ggccccagtc aaaccgggac ctcagtcttt cttcaatatt ttctatctct
    12781 gcgggaaatt tatcacttgt taatacaacc tgtttcatat tgtcatagag agcattaaaa
    12841 gtatgaaaaa attctgcctg tgttctttct tttccagcta taaattgaat atcatcaata
    12901 aggagaacat ccatcttacg aaacctgttt ctaaaatctt ccattttttc atagcgtaaa
    12961 gcattaatca attcattcgt aaaatgttct gcagtaatat agcagatttt ttccgggttt
    13021 gattttccgt gcttaacgag aaaattgcct atcgcgttga gaaggtgtgt ctttccaaga
    13081 ccaacaccac tgtagataaa caaaggatta tatgttttac ccgggtttgt tgccacggca
    13141 aggcaagcag catttgcaaa ttggttgctc gatcctacaa caaaattctc aaatgtgtat
    13201 ttcgggttaa aaacattaaa ttgagagggg cgccttagaa ccacacctct ttttccgtca
    13261 gtcctttgtt ctttttttat gacatattca atccggttgt ttgtgctcgt tatttccctc
    13321 attaatgtaa caagaataga ttcaaaatgt tgaataaccc aatccttgaa aaatgtattg
    13381 ggcaccataa gaacacattt attgtctaca tattcaacaa actctatagg ctcaatccac
    13441 gttttatagc tctcttcgct tataattttg ctgattttcg gcttaatttg tgcgaggata
    13501 tcgaccatcg acgatacttc ttttcaacac tttttcaaca tttgtggaaa acttaagtgt
    13561 ccttgaaagg ttaagattaa tcttaatata aaggaaaaat ttttgcaact gttttttagg
    13621 gattggaaag atacagtgca gacgtcatac aaagatattt taataaagaa gttgattggt
    13681 tccacggcct ctgttattat gtctcttatt ttgaatacat ttgctaaagc gatttcttgt
    13741 ctggttatat tcttcgccat gagtataatt gcccatgctg atgagtggga ttttgctttg
    13801 agaagagcta aaaaagagga taagccaaca atattatatt ttttcactcc ttattgcttc
    13861 tattgtcaaa ccatggacaa agaggtgctg gccaacaaag aagtcttcgc aatactcaat
    13921 aaaaatgttg tatatctgag aatcgatgtg gaaaagagag aagatcttgc tcggttttat
    13981 ggtgttagag gctatccgac cacagtgttt cttcaaccta acggacagca aataattcaa
    14041 ataccaggct acataaatag gaaagacttc aaaaaactac ttacctttgt cacgggaaaa
    14101 cattacaaaa caatgaaact cagagagttt ctccaaaaaa ccgcctgttt ggaaccatta
    14161 aagaacagac ctgaacccca gcagattttt catccaatat ggaagggtta aattatcaac
    14221 ggccacaccc cttgactttg aggcgtgtat gaactcgcgt tcattaacca taatgcctac
    14281 gtggaaatcc cctttaattc taaacacaac aagatcacct ggtaatacag aatccaatga
    14341 tacttcgatt ccgcttcgac cctgctcttc tgctgttggc gcaagggata accccacctt
    14401 tttataaaca taggagacga gaccgctaca atcaaaatag tcaggtcccc gccccccggc
    14461 tctatatgtc ttgccgagaa gatcgataga tgtgctaaca acgtgggctc tcaatcctga
    14521 tggtggtgca acggttctga cttttatagg cacacagcta aagaccacag aaacaaagca
    14581 aagaagaaaa acaactgtcc tgaacctctt ttgcttttca aacaacacta tttgtgtgtc
    14641 tttcgtattc atcaatgatc tcaatcattt caagaagcga tctgccgcat atcgcccgat
    14701 gaataacgct tgaaacatga tcttgagggc gaaacccaat ggcgagcact acctcttcaa
    14761 acatactggt atccccatca ccatccccaa cggcacaagc ctcttttctg ctgacaccaa
    14821 tgtctccgag gatctttttt actaaatacc cttttttgtt gaaacccaca tttattttga
    14881 tttcaccggt gaggtggccg ttttccgaca acaattcatt tgagaaggac atatctatac
    14941 caagatcttc tctaaccctt tcaacgagca aggaaaggcc ggttgacaaa attactgtaa
    15001 gaataccgcg ttttttaaga gcctttacca cctcatacgc accagagtga tacgtgatat
    15061 ctttcaacaa actgacaaca gaagaaacag ggaggccctt ccagagaagt gcatcattct
    15121 tgcagaattc atagtaactg atcttccctt ctctgaagag ggtttggtag cgctcggctt
    15181 gcttgtccca gatattgagt tttcggtgga gatattccca gctattccta gcttttgtaa
    15241 gagttccatc acagtcaaaa aaagctactt taatggtcat cgacctttta tagcatcaaa
    15301 aagagaagaa atctattttt ttgcaggtgg ctctagatta taataccagt gtagtgttat
    15361 gaaacctgtt gattgggata agagatataa ggaggggttt tacagacaaa caaaagaggc
    15421 acacccactg ctgcaacaat tttggcataa aataccgaaa ggaaatgtta tcgatattgc
    15481 tatgggcagc gggagaaacg ctttgtttct ttcagagagg ggatactccg tgtgtggtct
    15541 ggacaggtca tcagaagctc ttaagattgc aaggcaggca acggttgaaa agaaccgaag
    15601 tattttcttg atccgtggag acgcaggaag cctccccttt aagaccagtt ctgcatcagg
    15661 ggtgatcatt ttttactttc tcttaagaga tatcatggaa gagattattg gtctcctgaa
    15721 aacaggcggg gtgcttgtgt atgagacttt tttaaaaagg cagaatacaa ttgatcagta
    15781 caggaatcca gattttcttt tagaagatgg tgagctccta tcgtatttta aaaatcttga
    15841 gctccttttt tacgaagaaa ccatactgac cattgatgga aagcagaagg caacagcgag
    15901 atttgtaggg aggaagcgat gttaattgaa tgggatccaa ctcgaccgaa gaaaaaagcc
    15961 accgatctta tccgaaagac ccttgaagag ggtggtattg tcggatatcc aaccgacacc
    16021 ctgtatggta tgggctgcga tctcttcaac atcaaagcca taagaaaact tcaccagata
    16081 cggcggctac ccacaagatg ggctctcagt attatttgca gagacctgaa ggaggtaagc
    16141 gagtatgcag ttatgagtga ttatgccttt ggggctttga aggagtgcct accagggcct
    16201 tacacgttcg ttctcaaggc aaaaaaaatt ctgcctaaac ttctgatgac agaaagaaaa
    16261 gaagtgggcg tcaggatttc atcccacccg gtaccgacag ccctaaccag acttatagaa
    16321 aggcctatcc tgaacacaag cgcaagggtg tcaggtcaag agccttttac cgaccctcgt
    16381 gacattgaaa aggccttcaa agggggtata gacctggtca ttgatggtgg aatcattcct
    16441 gcccaaccgt ccacggtcgt tagactcaac gatgacaata tcgaggtgtt gagggagggc
    16501 aagggttctc tcaagaatct tctgatgaga tagcattctc ctggctcaaa gcctcttcca
    16561 tggactcttc aaagtcctca ccgagttcgt ccccaaactc ttttcccata cggcgcatca
    16621 cccgttcaac actcctcgga tcattttcat caaggtctga gaattttgat gggtcaagca
    16681 tgctttcaag gcgctgctct tcgctcttca agactgtcac cctggaaata accctgatca
    16741 cgtccttgtt tccacagtgc ttgcaatgag ggtcgatttc ttcggttatc ctcagaagca
    16801 aaaaagatga gagtttttta cacttcttac atcgatattc atatataggc attgaacccg
    16861 ctcctgatgt tcatggagtt gtaaaacacc aaaatcaact atgattatag cgacaagaat
    16921 gcggtatttc aagaacaaga ttccccagaa tgggattttt acacaataca ccctccgtta
    16981 aaaaaaaacg ataaacagat taaaaagaaa gataacccgg acagcgaaag gggattgatt
    17041 tttggaataa ccttgccagc actctggtct ccattcaaca tacgtactag ccataacttt
    17101 gtcagccaaa gcacataacc ggaaacattg ggaatggact tacccggagg acctgcacag
    17161 catgttcgtt ctcaaaaaaa ctcagatcaa agttttataa tatggcacca tctcgtacca
    17221 ggagttttct ttttgtacag attgtaggag gcaggcatac ctgattaaag cccgatcttt
    17281 tcattaattc ggtgttctgt gttctgcttc atttcctcaa tctgtataac catatccttt
    17341 aggcacaaat catgaaaaat ggcactgcac ccatcaaagc atcggtggcc ccttgttgcc
    17401 cagggctctg ccatcagcct gttcatcggg gattaggcgg gctgtgtcaa gctgcttttt
    17461 atagaatacg atgatggtcc tgcaaaattc aatgcgttcg atatacacaa tacctatgtt
    17521 gcccaaaaac actactcttg ctttgcgaat ctccaatttg acgagccctc tccagttgtt
    17581 tttatagaaa ctaatggctc tatggtaata attgaggcca agatataggt tgccaacatg
    17641 gcccagtaat ttttcttcct tggctttgtc cacaattcgg cggtatattg ccagagcgct
    17701 ttcatataag tcaagggcct ttaaatatac accgagctgt gtcgaggtgt taccaaatta
    17761 accagaaccc agcctacgcc cgaagtgttc ctagtctgac catggatcac aagagcattt
    17821 ttgtaaaact caaaggcttt tcatattcac ccatcaaaca gaaatatgac caaagtttgt
    17881 ggaaacccac agggattatt gaacgacctg ctgcgttccg tcaataactt tggtgggctg
    17941 ttgcacgata cgccggggca caaaacagaa tcaattttca gtatcactcg agtacaattt
    18001 ctttaactgc tttggcgatt accggtctcg gacttaccac acaagctacg gggacttgac
    18061 ccacgccacg cctcctttac tggcaaagga atagataaga aaacagccgc ttactttgaa
    18121 gctgggtttt gtgctaaagg tttaatggcg gactggatcg ccatacttat tcacagtgcc
    18181 cagggtgagc tgttagacta ctgtggccgg accatagacg aagaacgagc cagaaacaag
    18241 ggcaaataca agcaaacgac aggctttgct aaataaacaa taaacggttc ataaccttca
    18301 atgccaggac ccgggtgcaa agcacttatg ctggtcgaaa gccactttcc agcattcaaa
    18361 ctctacgagc tcggctttag aaacgtggtg gctttaatgg gcttggtttt aagcaacgag
    18421 caggcggctc tgattactga tttcctgggt ccagatggcc gggtaatact gggcttgaat
    18481 tataataaag acggagctaa atgcgcttaa gacgctctga ccaggctaaa aaagcgactc
    18541 tttgttaaag tggtggatgt aagcaaatat ggaaaaatcg catcttttaa cactcgccga
    18601 gtccatctct ttactgttat aattactgat taaacattca aggagtaagt tatggcatca
    18661 atggaaacaa atgatttccc ggttgagaca atttccgctc tggccaatat ttgggctgac
    18721 tggctcgtcg ggtttcatct tgctgttgcc caaataacca aaacaataat agacgctggt
    18781 gaagagaaca tagacgagta aagcgggctt atattctcga ttgaaaagat cgttggctta
    18841 accaaaggtg gccttgaagg aggaaacgat gtttatggtt attatcaagt ggaaagacgg
    18901 tacaagtagt agcatgaatc ttagtatcca gcatgcccca gccaatccga cggaagaaga
    18961 ttttaaagac ctattgacga tgacaaaagc ggtggttgat acagctctta atgacggttc
    19021 atttccaggg cggtccaaga aagacctgga cactatcgaa atcattacca ccagtgccaa
    19081 ttaaatcaac ataaaaccac atctgtccaa aatagatttt caggggcaac tccaaaaggt
    19141 acagctaatt atcccaggat ttaaacgaaa c
//
LOCUS       DAOOMC010000048        19066 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdus sp. isolate AMR_MDS_2685
            NODE_2939_length_19066_cov_5.697228, whole genome shotgun sequence.
ACCESSION   DAOOMC010000048 DAOOMC010000000
VERSION     DAOOMC010000048.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864760
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdus sp. (wastewater metagenome)
  ORGANISM  Syntrophorhabdus sp.
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae; Syntrophorhabdus.
REFERENCE   1  (bases 1 to 19066)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19066)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,729
            Genes (coding)                    :: 2,708
            CDSs (with protein)               :: 2,708
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 7 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19066
                     /organism="Syntrophorhabdus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2939_length_19066_cov_5.697228"
                     /isolate="AMR_MDS_2685"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2699756"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            503..2077
                     /locus_tag="PK125_06470"
     CDS             503..2077
                     /locus_tag="PK125_06470"
                     /inference="COORDINATES: protein motif:HMM:NF025890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HPB37786.1"
                     /translation="MKDFTHGGTTCQDCHSDVTSLPHDERLKKPSCKECHRKTAEEHD
                     AGVHGAAKVECKTCHTTHVITKSRKSCSDCHGDASHSSLPSKNKHLEKLSCLSCHSPV
                     KNSSIKTTLQVKRKGLISKASIDLDGNNTIDISEWDNLQAVLSKTFKSSPIIKKSYFA
                     ESDVHAIMKKPQPCKACHIDRQLFGQAKLFIQGAVKFEIFVDPSIFIPEIPSIETYRK
                     TVHGQKGVQCSDCHVSQKNIDDCVCIKCHQDIRKVYKDTVHSQKGAIQCIACHNPHRI
                     RAYKELTAKERLAVCSRCHKDYIQTHTWLPNTTLHFKYLECSTCHSPKSAKSMVFYLS
                     TKKGDKEERVDYKTLESFYGKNILMTPFLDKNKDEVVDSQELTGFFRDVRDRLSGNAF
                     IGSSIIVTRVHHDYSVKRQKERICATCHSDQAPFYESMFFVLPEDGFHMYVPVKGTIL
                     SAMPISVFVDMSLLGQQKATWADVKGLFTLKPGEFAPYAKELGFKWIDLIAIGFGAII
                     IFFILVHTLVRIIIRK"
     gene            2074..2709
                     /locus_tag="PK125_06475"
     CDS             2074..2709
                     /locus_tag="PK125_06475"
                     /inference="COORDINATES: protein motif:HMM:NF013460.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b/b6 domain-containing protein"
                     /protein_id="HPB37787.1"
                     /translation="MRKVFIHPISVRIWHWINAGLIFFLIITGVQLRFSWLTLFQYHH
                     AVFTHKIAGFILAGSFLYWLAYYLITGNFKQHYIFHRKDAKGMVRQAIYYTFTMFKHQ
                     KSPFTPSSQERFNPLQKIAYLSVMLIFTPVILVTGILFSDIVCFLGIINYIGGLRILD
                     AIHVATGYVFFLYLIVHLYMSTLGHRVVSHVIAMITGYVEERDETKQEQVE"
     gene            complement(3184..3462)
                     /locus_tag="PK125_06480"
     CDS             complement(3184..3462)
                     /locus_tag="PK125_06480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB37788.1"
                     /translation="MIEIELEPSLSHCIETVTKIEYERVLSLLLKGKEEDARLAEELE
                     LLRVFLESADFGQLRCRCEERLAAGKRALVRLMSSSLPPKYRVEFVET"
     gene            4282..5343
                     /locus_tag="PK125_06485"
     CDS             4282..5343
                     /locus_tag="PK125_06485"
                     /inference="COORDINATES: protein motif:HMM:NF024827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiheme c-type cytochrome"
                     /protein_id="HPB37789.1"
                     /translation="MIPKTYHKRFLVDKTVLDKDPHFINGCSYCHRGDEKSIDQEKAH
                     TGIIKKPSADLKACSDCHEGITKSYQDAPHYTVKGMLNKASKGFSKKIDGIFPEKVFE
                     QSCKSCHASCGDCHVSSPSKDGVGRGLIKGHRFVKRDEGKTCGVCHGGRVYPEFIGKH
                     TKSPDVHFQKVMTCVDCHKKNQIHGSPGSIRKNEIKGQNSPKCIDCHKLGREVKTTAR
                     LAHTRHEGTVSCYGCHSQGDYINCYNCHLDKGATSKLGFFLGVSPDDKKTLTTLRVVP
                     SLSSTFLSAGIKVKSFHEMPDYRAASVHNVQKITERTRSCDTCHIKRKGFLSKDTLMK
                     NGSEANKHLIFKLLPLNIN"
     gene            5374..6141
                     /locus_tag="PK125_06490"
     CDS             5374..6141
                     /locus_tag="PK125_06490"
                     /inference="COORDINATES: protein motif:HMM:NF012790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HPB37790.1"
                     /translation="MVSIFHRKSLRKAAVFIMLFILPFLCSCKATHTQDKSQKNIDSP
                     TAVYLIKEKKAAVIDTMSYLECMDHRIPGSHCIALEEIDKKLLPAFPNKSQLLIFYCE
                     SDKCSRAGQAYEKAKLLGYESVYILEDGLVAWKRAGYEVETIQRVKRAPVISIKSVRL
                     KEFIKERKDTFVLDIRSEDAFKANHLDGAKNIPFFMLHKRLDEIPKNVPVIVIDENGK
                     RSFLACCYLINNGVKDVIRLFGGMETMVRQTRNKDNT"
     gene            6138..7994
                     /locus_tag="PK125_06495"
     CDS             6138..7994
                     /locus_tag="PK125_06495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB37791.1"
                     /translation="MMKKCRVLILIPFLGFFLFAVSAYAATLKIQCSDCHDSEKFTTG
                     FKGSVHGNTGCTSCHAGFKSLDKHVIFKEKPALISCGTCHSEIEKQYRNSFHSLYEDF
                     KCYDCHYDIHTLKPEKENLKIAVTKNCTKCHTNDEYALSGHGAAVMKGNKDTATCSDC
                     HGLHDTKAYHTSGETYLLEAREFYNKTCKACHSNKEMMERNNISPNAVKYYEETYHGK
                     VQDLGYPTSVAGCADCHTNHNILPKSNPASTIHPKNLTQNCGKCHPGIHPRFAEYKAH
                     PDYRDKERYPQLYVSFLLMAGLLLVTFVFFWTHTLLWWRKVYWERHQMEELGIKLESP
                     FTHKEGIERIKRFSVTDRIMHVLLIISFFGLVITGFPLKYHSTPWAGAIIKALGGAHN
                     AGLYHRAAALILIGLFLYTLLKCFRFAFPKGFRKEGWRGFSARIFGPDSLMPNAQDWR
                     QFKAMVKWFFGKGKPPTFGRWTYWEKFDFLAVFWGMIAIGGSGVTLWAPGLASWVYPG
                     WVLNIASIVHSEEALLAALFIFTVHFFNTHLIPSKFPMDTIIFTGSYKLEELWRFRTP
                     EYERLVKEGRLESLKVKHPSITLKIISSMFGHASLWIGLLFTLILLWTFFFA"
     gene            complement(8040..8876)
                     /locus_tag="PK125_06500"
     CDS             complement(8040..8876)
                     /locus_tag="PK125_06500"
                     /inference="COORDINATES: protein motif:HMM:NF024793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HPB37792.1"
                     /translation="MSHLDFLLGQKEGFALVYGDVGLGKTTISRIFLSSLDENIYNTA
                     LILNPIMDEDELLRTILKELGLKTNGSSRKELYDALECFLLKEHRMGKETVLVIDEAQ
                     LLSNELLEFIRVLSNMETEKEKILRTVLFAQPELVERLKEPSMRHLAQRITVTYKLKP
                     FSERDVRHYITYRLIKAGSKGTPGFTKGAARLIYKASNGYPRLVNILSDRCMLSVYSK
                     SKAIVNKKTVVRVLKEENISLTSVIKNPLLVQPYRIIVLCLVGAILILLALYVVNVLG
                     LR"
     gene            9056..9739
                     /locus_tag="PK125_06505"
     CDS             9056..9739
                     /locus_tag="PK125_06505"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HPB37793.1"
                     /translation="MMLSIIIPAYNEAPFLPEVIRRVEETPYEKEIIVVDDGSSDGTR
                     DYLESLSTERVKAIFHEENMGKGASFRSGLSLVTGDIIIVQDADLEYDPKDYPVLLGP
                     ILEGKADVVYGSRFLGGPHRVLFFWHSVGNSLITLISNMLTDLNLTDMETGYKVFRRE
                     VFKDIRIESNRFGFEPEITAKVAKKGFRIYEVPISYSGRSYQEGKKITWKDGIKAIFT
                     VLKYNLFRR"
     gene            9799..10164
                     /locus_tag="PK125_06510"
     CDS             9799..10164
                     /locus_tag="PK125_06510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB37794.1"
                     /translation="MVVLIMGILASILMYRYNISQEAGAIVAKDALIADILYTQAKAM
                     ASETQQSITFTIGSGIYTMQGEKKKLPDGVVVTSTSLPGNTLLFNTLGEPMFGISDRT
                     VVLTGGRTLTVYAITGKVE"
     gene            10165..11139
                     /locus_tag="PK125_06515"
     CDS             10165..11139
                     /locus_tag="PK125_06515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB37795.1"
                     /translation="MAKNLTVGGFTLLELIVFIVVAGIFIPMAYIAFMATTRASMNPE
                     EVIIARFLAESKLEDITKDTFLNLQGGQTGYVAVPGYAGYQWRWTIQLIAYQGRTTHG
                     SPKLGIPEMWRASTVYRTGDYITPTIATPATHFYRCIPPERWQSNTRYDLNSYVSPIV
                     PNNLSYRATARSSFPSWQANHAYVSGDYVIPTVPNGRSYRCTGTGTSGSVEPSWPSTG
                     TIADGTVIWLENTNTLTTGPQEPAWPNQSASASSVDDGSITWIREAMKSASTEPSWPP
                     IRSSIVNDGSLRWQESTCYKLVTVYVREPKGLEYAVNSLVTARPGTYP"
     gene            11136..11759
                     /locus_tag="PK125_06520"
     CDS             11136..11759
                     /locus_tag="PK125_06520"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HPB37796.1"
                     /translation="MNMRKGFTLIELIIVIVISSMVGVFTFSFIYSSIQTYRLMRTQS
                     QIYQEASYVLDRITRELRDATYNLSSTRGISFTKAHQTPADSNTFVRYYQSGTSLFRC
                     SDSVSGHICLFNPDSSPTNKAISSNIAAFEVLHSPNVECNPSNPPTCQDDSFSITLKM
                     IKEGQTIVVGATITPKNYCSYGPTSTSCSSSDYTNRSFNRDYRDVVN"
     gene            11746..12117
                     /locus_tag="PK125_06525"
     CDS             11746..12117
                     /locus_tag="PK125_06525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB37797.1"
                     /translation="MWLTEKGFSLIVLIIAITLTSILGVGVVSFMSAKHKTLVPQTQT
                     CQAYAIAHAGIEFAIRYAYDNKDDNDFPDAHFPKTVSLGGGSFTITYDLTNNLVRSVG
                     VYETASRTIELTNFRSYANIP"
     gene            12306..14006
                     /gene="pilM"
                     /locus_tag="PK125_06530"
     CDS             12306..14006
                     /gene="pilM"
                     /locus_tag="PK125_06530"
                     /inference="COORDINATES: protein motif:HMM:NF022554.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilM"
                     /protein_id="HPB37798.1"
                     /translation="MEGATQAPVIDMDILGIDIGTVSVKYLRIRGKGVIISHGDYPHK
                     GGWEDLNFVLTDIKDKEGTDVEIAIAVSSPDILKRSFSIPVMPRDEAKEAIEWAASKV
                     VTTSLEDMIYENIMLGQINERGLQKEEVFFIGANKEYINILLSVFETVGFRRVNIITD
                     CGVLYLPIIAEKKEGTVAVVDIGGRQTGIYIFDEKRLRFMREILTASESFIDTLMSEF
                     GLSYDEAEQYIIEKGFNEESKNILSFPLDRLTGEVQRTFNVYTRKHPERPITKIYITG
                     RASRIPNLISRIQDVFMEEVERLMSPIEIDEQFLPLYALCMKTEPLVNLLPEKLKARK
                     REMTFQSWLRLATICVVAILLIPSIIMLTDLRKMQVFIKSEQADVASKKEQLRLLGSI
                     TTDSRYGELMALNKEVGKKDVTFVTLLKYLSSRLPRDVYIREIGFTDKNKRFDSTIKD
                     LSREHSTKEGSGIRETAKTTPKEDAEASSPSKGAIPATGQSTLAIIPGEPDYAVTLRG
                     YIFGDIDVLEPALVNLVISIERYALLRNVEVSMRETKEIRGKKALEFIITGQCIRHEI
                     "
     gene            13996..14538
                     /gene="pilO"
                     /locus_tag="PK125_06535"
     CDS             13996..14538
                     /gene="pilO"
                     /locus_tag="PK125_06535"
                     /inference="COORDINATES: protein motif:HMM:NF016253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA];
                     GO_process: GO:0043683 - type IV pilus assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 4a pilus biogenesis protein PilO"
                     /protein_id="HPB37799.1"
                     /translation="MRFKIIYLWFSLPVLLAATWFFAFYSPVSSFIEKQRAELSAAKR
                     TGEMMGKALRDVLETRKKDATARLSLDGISKNVPAYQQFPTLIRAITESGKKEGIVFD
                     LLNSNALPYDSGQASPLMKPALGVGFKGKFLDIGKFLEAVEKQKGFKRIVEGRLSYAD
                     KDYPILTGNFLIEFVVWKGD"
     gene            14543..14989
                     /locus_tag="PK125_06540"
     CDS             14543..14989
                     /locus_tag="PK125_06540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB37800.1"
                     /translation="MKVSKSILVALILAVLFAAYSFLFTDKKVQSTFPVPVTNIAGTT
                     PQAPYSTTGSKTPRLNVDTLNLSWQNDPFSLPKSVMEKKAEKPKIALKLVAIMESNAG
                     RYAIIGGEIVKKGDKIGEEKVVEIHNDRVVLIRNNTKRILSIEDTR"
     gene            15028..16500
                     /locus_tag="PK125_06545"
     CDS             15028..16500
                     /locus_tag="PK125_06545"
                     /inference="COORDINATES: protein motif:HMM:NF012485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secretin N-terminal domain-containing protein"
                     /protein_id="HPB37801.1"
                     /translation="MKRLFIFICILFLCAACRHVQTATTPSAPIAVKPVEIMSPVVPQ
                     VEKESKTLAESYAERFSFTLKEADVKDVLRGLSKQTNYNVVVEPDVKGVCTVDLKDVT
                     LMKALEYILEPLDFTFKIEEKTIYVSKPKLETKFFSLNYVALKKIGTSSVIGSAGGVG
                     SLGTAGSSSEYRSVEIKTETEADIWKNIEDNLKVMLSKDGKVVVNRQALMVLVTDYPK
                     NLKQIASFLNAVEGSLHKQVMIEAKIIEVQLNDASREGVNWQFIEGRIGEFFVNTKQV
                     FLNPNIINVPSTTQATATTPYFRLFVGNKHLNIDNTFIDLLKTQGNINVVSSPRIATL
                     NNQRAVIKVARQDVYFDEQQSVGVVSTTSLATYTPKFITVGLMLDVTPQIDNNGNIVL
                     NIHPMLTEKVDVALSPSGAAVPILDVREADTMVRVREGETVIIGGLMKDRKRISDTGT
                     KGLMDLPLIGWLFRLKESEAQKNELVVFLTPRIMSTGETP"
     gene            16497..17561
                     /locus_tag="PK125_06550"
     CDS             16497..17561
                     /locus_tag="PK125_06550"
                     /inference="COORDINATES: protein motif:HMM:NF024579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB37802.1"
                     /translation="MSVILDALKKAQKERKDMTKNLPYNPREKSQKSRWVLYTGALLV
                     FCVLLVFFLIPDMKKAPKIMIEVNPVHTPQPISQPVAPEKTAAVVQSTVAVNPLASEQ
                     LDRRDNITGHVKPVKTQKNIASLLSKNASGMTKSRVETLPEKVWHQDEARVLISSTDF
                     EKINLKFHKAVKETERGNISEAKQLYHAILAEQPSNVETLNNLGVISIKEGNTEEALF
                     YFNKVFQYNKNYGKAYNNVGLVMMKGGQKGLAEEYFRKSIELKKDDVEPVINLAALLR
                     TEKRYEEAARLLENLLRTSVTSKPLYLSYALIKDDLGHYEQAIKYYRLYLRGSMGDSQ
                     RSDVIKRLEVLENAQSSRTH"
     gene            17829..>19066
                     /locus_tag="PK125_06555"
     CDS             17829..>19066
                     /locus_tag="PK125_06555"
                     /inference="COORDINATES: protein motif:HMM:NF012652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HPB37803.1"
                     /translation="MARMIRKKLGEILVGAGKLSDESLVRALDEQKKYGEKLGKILTK
                     MEFLTEKEIIDTLSQQLGIPIVSLDGRDIPMDLIRLFPEEVWKNYMVIPFEKHFNVLR
                     IAMVDPLDINAIDEVARLVKMEVEPCIVTEGDMKRALAKYYGAKTIVEQTLEDMGEDV
                     DLTLIKDASLEEVDKISIDMTEQEPVVRFVNSVLTQSLADGASDIHVEPLERKMRVRM
                     RIDGRLREIPAPEKKMFLPIISRIKILAGMDIAKTRIPQDGRFDIREGAKEVGVRVST
                     FPTIHGEKAVLRLLDKGAALYGIDKLGLLPDDEKTLKNVVKRPYGFILSTGPTGSGKS
                     TTLYAILNFINSPEKNIITIEDPVEYTIDGLAQTQVNPKAGLTFDSGLRAILRQDPDI
                     IMVGEIRDKETATIAIHSALT"
BASE COUNT         5752 a         4012 c         4160 g         5142 t
ORIGIN      
        1 cgttggtctt ggcatccatt attctcctta ttatataatg aatatatatt catatagctt
       61 cctgtcaagt attttgtttc tcattctaac ctttattgag ctgcttttaa ataatttctg
      121 atcaatcaag ggggaaaaga aattgttctg agctaaatga tgcatataaa cttttgtgtt
      181 catgttcttc tgatggtgcg atttgtatca gtaacttgat tgccagtgat gttcgaaata
      241 tgataccaaa taaaatcgaa aaaaggagat gttttcttac gggtcaaagg ccacatgccc
      301 ctctaaagct agttagaaac atttgatttt acctaaggat atattttata aattcaaaca
      361 tttgttagtt ctctggtcgg aaaaaatgtc ttatcttcgt tactatcata ttaaaatcct
      421 attgtttttg attattcttt tgctttcttt ctccgccttt ggcgcaactg actctgataa
      481 ttgcctgggt tgccatgatg gaatgaaaga ttttacccat ggcggtacaa catgtcagga
      541 ctgtcactct gacgtcactt ccctgcccca cgatgagagg ctaaaaaagc catcttgcaa
      601 ggagtgtcac agaaaaactg ccgaagagca tgatgccggt gttcacgggg cagcgaaagt
      661 agaatgtaaa acctgccaca ctacacatgt tatcactaaa agcaggaagt cctgctctga
      721 ctgtcacggt gatgcgtccc atagttcgct accctcaaaa aacaaacatc tggaaaaact
      781 ttcatgcctc tcctgccaca gtccagttaa aaacagttct atcaaaacca ccctccaggt
      841 aaagaggaaa gggcttattt caaaggcatc aatagatctt gacggtaaca acacaattga
      901 catcagcgag tgggataacc ttcaagcggt cttgagcaaa acatttaaaa gttctcctat
      961 aatcaaaaaa agctatttcg ctgaaagcga tgtccacgct atcatgaaaa aaccccaacc
     1021 ctgcaaagca tgccatattg accggcaact ttttggtcag gctaaattat ttatccaagg
     1081 tgcagtaaag tttgagattt ttgttgatcc gtccatcttt atcccagaaa ttccttcaat
     1141 agaaacatac cggaagaccg ttcacggtca aaaaggggtt cagtgttcgg attgtcacgt
     1201 atcccagaaa aatattgatg attgcgtctg tataaaatgt catcaggaca ttcgcaaggt
     1261 ttacaaagat acggtccatt ctcaaaaggg ggcaatacag tgtatcgcct gccataatcc
     1321 ccatcgtatc agagcttaca aggaacttac cgcaaaagag aggttggctg tatgttcccg
     1381 gtgtcataaa gactacattc agacccacac atggttgccg aacactactc ttcacttcaa
     1441 atatcttgaa tgttccacat gtcacagtcc caaatctgca aagagcatgg ttttctactt
     1501 gagcacaaaa aagggcgaca aagaggaaag ggtagactac aaaaccctgg aaagcttcta
     1561 tggaaaaaat atactaatga ccccattcct tgacaaaaac aaagatgaag tagtggattc
     1621 acaagagctg acaggttttt tcagggatgt tagagatagg ctttcaggca atgcctttat
     1681 tggaagttca atcattgtta cgcgcgtgca tcatgattat tcagtaaaaa gacaaaagga
     1741 acgcatatgt gcaacatgcc actctgatca agctccattt tatgaatcta tgttttttgt
     1801 cttaccagaa gatggatttc atatgtatgt tccggttaaa ggcactatcc tttcagcaat
     1861 gccaatatct gtttttgttg acatgagcct tctcgggcaa caaaaggcaa catgggctga
     1921 tgtaaaaggc ctctttactt taaaaccggg agaatttgca ccctatgcaa aggagcttgg
     1981 tttcaaatgg attgatctta ttgccattgg gtttggtgct attattatat ttttcattct
     2041 cgtccatacc cttgtaagga tcattataag aaaatgagaa aggtttttat tcaccccatt
     2101 tccgttagaa tctggcactg gatcaatgcc ggacttattt tttttctcat cattacaggc
     2161 gttcaacttc gtttttcctg gctcacgtta tttcaatacc accatgctgt atttacacac
     2221 aagatagccg gttttatttt ggctggatct tttctctatt ggctcgccta ttacttaatc
     2281 accgggaatt ttaagcagca ctatatattt cataggaaag atgcaaaagg tatggtacgc
     2341 caggccattt attatacctt tactatgttt aagcaccaga aaagcccctt taccccatca
     2401 tcacaagaaa gatttaaccc cttgcaaaaa atagcgtatt tatcggtaat gctcattttc
     2461 acacctgtaa ttctcgttac tggtatcctt ttcagtgaca ttgtctgttt tctgggtatt
     2521 atcaattata ttggaggact cagaattctc gatgcaatac acgtggccac aggatacgtt
     2581 ttcttcctct acttaattgt ccacctttat atgtcaaccc tcggtcacag ggtggtttcc
     2641 catgtaatag ccatgattac cggatatgtt gaagagcgtg atgaaacaaa acaggaacag
     2701 gtcgagtgat tattatagca ccctcaatat aacaacaatg cttcagtttc tgaaatagaa
     2761 cagtatggag aaaactggcg ctcgctgatt tattataccc tgatcattac cccgaaagcc
     2821 cagggttgaa gagaaggtca aaatcgtcaa tgatgcctgt tctatattag gaaatccaca
     2881 tagccgggga gaataatacg acctgcatag tttccattcc ttggtatgga atattttcat
     2941 agtaggtaaa caataataga tgcagatacg tcagatagag gaagttaatg tggctgggaa
     3001 cgtatggagt actgtccaca ggtatatttt atctgcgtaa agcaataaga gaataaacaa
     3061 gacacgttgc ataaatggaa tcgacagaat gccttatgtg taacttcggc tctaataatc
     3121 tccaagtcca ttttttccac acagcaattt tgcctgaggc ccacagatgt cctacaggtt
     3181 cttctatgtc tcaacgaatt cgaccctata tttcggcggg aggctactcg acatcagcct
     3241 gaccaaggct cgtttgccag ctgccaacct ctcttcacat cgacatcgta gctggccaaa
     3301 gtcagccgat tccagaaaca cccttaaaag ctccaattct tccgccaacc gagcatcttc
     3361 ttccttccct ttaaggagca ggctaagtac ccgctcatac tctattttag taacagtctc
     3421 gatgcaatgc gaaagacttg gctcaagctc gatttcaatc attctgcaag ccatctacat
     3481 acattacact gtgtttccag tagtaagacc ggatttttta tttccgctgg tacaagatat
     3541 tgtaaataaa catatataat ctttttatgg taattcttct gagcaattat actgaagatt
     3601 ctaaacaaaa atgaatatat atgcactaaa attataaaaa tctggctcaa tcgtgtcaat
     3661 aacaaaaaag ttggttagct ttccatctaa gacaccccat ttctccgctc ttgatcatta
     3721 caacataaaa ctataaagcc ttgaaataca cagccttcaa aaacacgcgt aatcctcttt
     3781 ttgcaaattc aaaaatatga ttctcccgga tgttgcaatc aaagaacata attgaattgg
     3841 catcatagcc cggctacatg aaagttatca ggcctatggg aaagttatgc ggttacaaaa
     3901 tcacatggca ttttgcatta tcgccactgt catgtttgca ttatcgctgg gctgttgact
     3961 ggtaaagttg agtcatggta atcaacgcaa taagacttca taaattaagg gctagcaacc
     4021 ttgaacagcc ctaacagtct gattacaaat tttacaaatc ctaccttgac atgcatctct
     4081 gtatctgtta ttcaatcaat gagaatgatg aagttcgcgg ttctctttgc gatttttttt
     4141 gtatatttca cttgcgctgc ctcagcaagt gaggtgggct gcatagaatg tcatacatat
     4201 gaacctgttc tcgaagctct tgttacagtg cccacggtag cccctcaaac aggggttgga
     4261 ccaacaggtg gaccagctac tatgataccg aaaacgtatc acaaacggtt tcttgtagat
     4321 aaaaccgtgc tggataaaga tccccacttt attaatggat gcagttactg tcacaggggt
     4381 gacgaaaaat ccattgacca agaaaaagca cacactggca tcattaaaaa accctctgcc
     4441 gacctaaagg cctgtagcga ctgccatgag ggcattacca agtcctatca ggacgctcct
     4501 cactatacag ttaagggcat gttgaacaag gcatcaaaag gattttcaaa aaagatagat
     4561 gggatttttc ccgaaaaagt atttgaacaa tcatgcaaaa gctgccacgc atcgtgtggc
     4621 gactgtcatg tcagctctcc gtctaaagac ggagtgggta ggggtctgat aaaaggccat
     4681 agatttgtaa aaagagatga aggaaaaacc tgtggcgtgt gccacggagg gagggtttac
     4741 cctgaattta ttggtaaaca caccaagagt cctgacgtac attttcaaaa agtcatgacg
     4801 tgtgttgatt gtcacaagaa aaaccagatc catggtagcc ctggtagcat tcgaaaaaac
     4861 gaaataaaag gacagaacag tcctaagtgc attgattgtc acaagttagg aagggaggtt
     4921 aaaaccacag caaggctggc ccatacaaga catgagggaa ccgttagctg ttatgggtgt
     4981 cattctcaag gtgactatat caattgttat aactgtcact tggacaaagg agcaacctcg
     5041 aaacttggtt ttttccttgg cgttagtcct gatgataaaa agaccctcac cactcttcgg
     5101 gttgtaccta gcctcagtag cacttttttg agtgcaggga taaaggtgaa gagcttccat
     5161 gagatgccag actatagggc tgcatcagtt cataacgtac aaaagatcac cgagaggacg
     5221 cgctcatgtg atacgtgtca tataaagaga aagggttttc tttcaaagga tacactcatg
     5281 aagaatggtt cagaggcaaa taaacatttg atttttaagt tacttccttt gaatattaat
     5341 tgaatcacta tgggctggat tagcgtggac ttaatggtgt ccatatttca tcgcaagagc
     5401 cttcgtaagg cagcagtttt tatcatgctc ttcatcctgc cttttctctg ttcctgtaag
     5461 gcaacgcaca cacaagacaa aagtcaaaaa aatatcgatt cgcccactgc tgtttacctg
     5521 ataaaggaaa agaaagctgc tgttattgac accatgagct accttgagtg tatggaccac
     5581 agaataccag ggtctcactg tattgccctt gaagagattg ataagaagtt gctccctgcc
     5641 tttccgaaca agagccagct cttaattttt tactgtgaat cggataaatg ttcgcgggct
     5701 ggtcaggcct atgaaaaggc aaaactgctc ggttatgaaa gcgtatacat ccttgaagac
     5761 ggtttggttg cctggaagag agcaggctat gaagttgaaa cgatacagcg ggtaaaaagg
     5821 gctcctgtaa tctctattaa atcagtaagg ttgaaggaat tcataaaaga aagaaaagat
     5881 acgttcgttc tggatattcg atctgaggat gcatttaaag caaaccacct cgatggtgcc
     5941 aaaaacatcc ccttcttcat gcttcacaaa agactggatg aaatcccaaa aaacgttcca
     6001 gtcattgtga ttgatgagaa cgggaagagg tcttttctcg cctgctgcta ccttataaac
     6061 aatggtgtga aagatgtgat aaggctcttt gggggaatgg aaaccatggt tcgtcagaca
     6121 aggaacaagg ataacacatg atgaagaagt gtcgagtatt gatccttatt cctttcctgg
     6181 ggttctttct ttttgcagtc agcgcatatg ctgccacttt gaagatacag tgttcagact
     6241 gccacgatag tgaaaaattt acaacaggat ttaaaggctc ggtgcatggc aacaccggat
     6301 gcacaagctg ccatgctggt ttcaaaagtc tggataaaca tgtaattttc aaagaaaagc
     6361 ctgccttaat ttcctgcgga acgtgccaca gtgagataga aaaacagtac aggaatagct
     6421 tccactcttt atatgaagac ttcaagtgtt atgactgtca ctatgatatc cacacactta
     6481 aaccggaaaa ggagaatctc aagattgcag ttacgaaaaa ctgtacaaaa tgccacacta
     6541 acgatgagta tgccttgtca ggtcatggcg cggctgtcat gaagggcaac aaagacacag
     6601 ctacatgcag cgactgtcat ggcttgcacg acacaaaggc gtatcataca tcgggcgaga
     6661 cgtatctgtt ggaggcgagg gaattctata acaaaacctg taaagcctgt cactccaata
     6721 aagagatgat ggagcgcaat aatatctctc caaatgctgt caagtattac gaagaaacat
     6781 atcatggcaa ggtgcaggac ttgggatacc ccacaagcgt tgctggctgt gcagattgcc
     6841 atacaaacca caatatactt cccaagtcaa atcccgcatc aaccattcac cccaaaaacc
     6901 ttacacaaaa ctgcgggaag tgccaccctg gcattcaccc gaggtttgct gaatacaaag
     6961 ctcatcctga ctacagagat aaagaaagat atccccagct atacgtgagt tttctactga
     7021 tggcaggact tcttctcgta accttcgttt tcttctggac acataccctt ctctggtggc
     7081 gaaaggtata ttgggaaagg caccaaatgg aggaactggg tataaaactg gaaagtccct
     7141 ttacccacaa agaaggtatt gaaagaatca agcgattttc tgtgacggac aggataatgc
     7201 atgttcttct cattatttca ttttttggtc ttgtaataac cggcttccct ctgaagtatc
     7261 acagcacgcc atgggctgga gctatcataa aagccttggg aggagctcac aatgcaggcc
     7321 tttatcacag ggctgccgca ttgattctta ttggattatt cctctataca ttattgaaat
     7381 gttttcgctt tgcttttcca aaaggctttc gcaaggaggg atggcgcggg ttctctgcga
     7441 ggatttttgg acctgattcc ttaatgccaa acgcacagga ttggcgtcaa ttcaaagcta
     7501 tggtcaaatg gttttttggg aagggcaaac caccaacatt tggtcgttgg acttattggg
     7561 agaaattcga ttttcttgca gttttctggg gcatgattgc tattggaggc tcaggtgtga
     7621 ccttatgggc gccgggactt gcatcatggg tttaccctgg ctgggttctc aatattgcga
     7681 gcattgttca ttcagaagag gcccttcttg ctgccctttt tatattcaca gtccactttt
     7741 tcaataccca tttaattcca tcgaaattcc ctatggacac aatcatcttt acgggtagct
     7801 acaaacttga agaattgtgg agattcagaa cccctgaata tgaacggctt gtgaaagaag
     7861 ggagactgga aagcctgaaa gttaagcacc cctcaataac cttaaaaatc atctcatcca
     7921 tgttcggcca tgcaagtctt tggatcgggc ttctcttcac ccttatattg ctgtggacat
     7981 tcttctttgc ttaaacatcc gctatcccgg ggcctcaccc ctgatccata ggcctctgtt
     8041 catctaagcc ccaagacatt cacaacataa agagcaagca ggatcaggat ggcgcccaca
     8101 agacagagaa caatgatccg gtaaggctgg actaatagag ggttcttaat caccgaggtc
     8161 aaggaaatgt tttcttcttt caagacacgg acaactgtct ttttgttcac aatggccttt
     8221 gatttagagt aaacagagag catgcaacga tcactcagga tgtttacaag tcggggatac
     8281 ccgttagatg ccttgtagat gagccttgca gctccctttg taaaacctgg agttcccttg
     8341 gagcctgcct tgatgagccg gtacgtaata tagtgcctga cgtctctttc gctgaacggt
     8401 ttaagtttgt atgtaaccgt gatgcgctgg gcgagatgtc gcattgaagg ttcctttagc
     8461 ctctcaacca actctggctg agcaaaaaga acggtgcgga gtattttctc cttttccgtt
     8521 tccatgttgg aaagaacacg tataaactca aggagctcat tggagagaag ttgtgcttca
     8581 tcaattacaa gtacagtctc tttccccatc ctgtgttctt tcaggagaaa acactccagg
     8641 gcatcataga gttcttttct ggacgaacca ttggtcttta agcccaactc ttttaggatc
     8701 gtcctgagga gttcgtcttc atccattata ggattgagga taagggctgt attataaata
     8761 ttttcatcaa gagaggacag aaatattctt gaaatagtcg ttttccccag ccctacatct
     8821 ccataaacaa gtgcaaaacc ttctttctgt ccaaggagga aatcaaggtg agacaaggcc
     8881 tccctgtgcg tctctgtttc aaagtaaaaa agcggatccg gagtcagtcc aaaaggtttt
     8941 tcactgagtc caaaatattt taaataaggt atttccatga acttaatatg acactaacgg
     9001 ctatttttta caataaatta ttgatatccc tatggtattc ttataaaatc agacaatgat
     9061 gctctccatc attattcctg catataatga agcccctttc ttgcctgaag tcatcagaag
     9121 ggttgaagaa acaccctatg aaaaggaaat tatagttgtt gacgacgggt cttctgatgg
     9181 tactcgtgat tatctggaga gtctgagcac agagagggtt aaggctattt tccacgaaga
     9241 gaatatggga aagggtgcgt cctttcgtag cggcctttca cttgtaactg gggatatcat
     9301 catagtgcaa gatgctgatc tcgagtatga cccgaaagac tacccggtac tcttaggccc
     9361 tattcttgaa ggaaaggctg atgttgtcta tggttctcgg tttttaggcg gacctcacag
     9421 ggtacttttt ttctggcatt ctgtaggcaa ctctctcata accctgatct cgaacatgtt
     9481 gactgacctt aacctcacag acatggaaac cggttacaaa gttttccgaa gagaagtgtt
     9541 caaagacatc agaatagagt caaataggtt tgggtttgaa cctgagatca cggccaaggt
     9601 agcaaaaaag ggttttcgga tttatgaggt tcctatttca tattcgggca ggagttatca
     9661 agaggggaaa aagattacgt ggaaggatgg tattaaagca atttttactg ttttaaaata
     9721 caaccttttc cgacgataaa taagctcatg tcaaaaaaat ggcaaaatat taatgggttc
     9781 actgcaatag aggcaatcat ggtggttctg attatgggta tacttgcttc catacttatg
     9841 taccgatata atatctcaca agaggcgggg gctatagtgg caaaggatgc ccttattgct
     9901 gatatcctat atacccaggc aaaagcaatg gcctcagaga cgcaacaaag cattaccttc
     9961 actattggct ctggtattta caccatgcag ggcgaaaaaa agaaacttcc tgatggtgtt
    10021 gtcgtaacaa gtaccagttt gccaggcaac actctactgt tcaacaccct tggagaacca
    10081 atgtttggga ttagcgatag gacagttgtt cttacaggag ggcgaacgtt gacagtttac
    10141 gccattacag gaaaagtgga gtaaatggca aaaaatttaa cagtcggtgg ttttacactt
    10201 cttgaattga tcgttttcat tgttgttgca ggcatattca ttcccatggc ctatattgcg
    10261 ttcatggcta caacgcgagc ttcgatgaat ccagaagaag tcataattgc aaggttcctt
    10321 gctgaaagca aacttgaaga tatcaccaag gatacttttc taaatcttca gggaggacag
    10381 acaggttatg ttgccgttcc aggatatgct ggctaccagt ggcgctggac tattcaactc
    10441 attgcatatc aaggtcgcac aactcatggg tctccgaagc tgggcatacc tgagatgtgg
    10501 cgcgccagca ccgtctatag gactggagat tatataacgc ccacaatagc cactcccgca
    10561 actcatttct atagatgcat accgcctgaa agatggcagt caaacacccg ttatgactta
    10621 aacagctatg tttcgccgat agtaccgaat aacctctctt atcgggctac agcacgttcg
    10681 agttttcctt cttggcaagc aaatcatgca tatgtttcag gagattacgt gattcccaca
    10741 gtaccaaatg gtcgttctta tcggtgcaca ggaacaggca cttccggatc agtcgagcct
    10801 tcctggcctt caacaggcac tatcgctgat ggaactgtca tctggcttga aaataccaat
    10861 acccttacca ctggcccaca ggagccggca tggccaaatc agagtgcatc agccagttct
    10921 gtggatgatg gcagcattac ctggattaga gaggcaatga agtctgcatc aacagaaccg
    10981 tcctggcccc caataaggtc gtctattgtg aacgatggta gtctgcgctg gcaggaaagc
    11041 acgtgttata aactggtcac tgtatatgtc agagagccga aggggttgga gtatgctgtc
    11101 aactcccttg ttacggcgag gcccggaaca tatccatgaa tatgagaaag ggttttacgc
    11161 tcatagaact gattattgtg atcgtcatca gcagtatggt gggtgttttt actttttcct
    11221 ttatttatag tagcatacaa acgtatcgtc tcatgaggac acaaagtcag atatatcagg
    11281 aagcatcata tgtccttgat cgaatcacta gagagcttcg tgatgcaaca tacaatctga
    11341 gcagcaccag gggcatttct ttcacaaagg cgcatcaaac ccctgctgac tccaatacct
    11401 ttgttcgcta ttatcaatca ggcacttcgc ttttcagatg ctccgattca gtatccggcc
    11461 atatttgtct tttcaaccct gactcttctc cgaccaacaa ggctatcagt agcaatattg
    11521 ctgcctttga ggttctgcac agcccgaatg tcgaatgcaa tccatcgaac ccgcccacct
    11581 gccaggatga ctctttttct ataaccctaa aaatgattaa ggagggtcag actatcgttg
    11641 tgggggctac aatcacacca aaaaattatt gctcgtatgg ccctacgtca acttcctgct
    11701 cttccagcga ttacacaaac agaagcttta acagggacta tcgagatgtg gttaactgaa
    11761 aagggctttt ctttaatcgt tttgattatt gccataacat tgacaagcat tcttggtgtt
    11821 ggtgtcgttt ctttcatgag tgctaaacac aaaacccttg tcccacaaac acaaacctgt
    11881 caagcctatg cgattgcgca cgccggcata gagtttgcaa tacgttatgc atatgacaat
    11941 aaagatgaca atgattttcc agatgctcac ttcccaaaga ctgtgtcttt ggggggaggc
    12001 agctttacga taacgtatga tctgaccaac aatttggtca gatctgtggg agtatacgag
    12061 acggcttcaa ggactattga gttaacaaat ttcagaagtt atgctaatat tccttaggcc
    12121 gacacgatag gcatggtagt tttgatgcga ggggtaagca ccatacaaca tacaaagaag
    12181 ggtctgcttt agggccatag aatggatatt ctcggcattg atataggaac aaattctcta
    12241 ccggtcgttg gaatctatac ggtgagcacg gcgaacatga agggaaggct ccaacagctt
    12301 agctggtgga gggggcgacg caagcccctg taatagatat ggatattctc ggcattgata
    12361 taggaactgt tagcgtaaag tacctcagga taagggggaa aggggttatc atctctcatg
    12421 gtgattaccc ccataaaggc gggtgggaag atcttaattt tgtgcttaca gacattaaag
    12481 acaaagaagg aactgatgtt gaaattgcca ttgccgtaag ttccccggat attcttaaga
    12541 gaagtttttc catacctgtt atgcccaggg acgaggccaa ggaggcaatt gagtgggctg
    12601 cgtcaaaggt cgttactact tccctcgaag atatgatcta tgaaaatatc atgcttggtc
    12661 agatcaatga acggggcctc cagaaagaag aagtattctt tatcggggca aataaagaat
    12721 acatcaatat tctcctttcc gtttttgaaa ctgttggttt tcgaagagtc aacattatca
    12781 ctgattgcgg cgtcctttat ctgcctataa ttgcggagaa aaaagaagga acagttgcag
    12841 tagtagacat tggtggaagg caaacaggca tttatatttt cgatgaaaag agacttcgtt
    12901 ttatgaggga gattcttacg gcatcggaaa gcttcataga taccctcatg agcgaatttg
    12961 gcctatcgta tgatgaggct gagcagtata tcattgagaa gggatttaat gaagagtcca
    13021 agaacatcct gtcttttcct cttgaccgct taaccggaga ggttcagaga acattcaatg
    13081 tctatacccg aaagcatccc gaaaggccaa tcacgaaaat atatataacg ggaagggcat
    13141 cgagaatacc aaacctgatc tcaaggattc aagatgtttt catggaagaa gttgaacgcc
    13201 ttatgagtcc gatcgaaatt gatgaacaat ttctaccttt atatgccctg tgtatgaaga
    13261 ctgaaccact cgttaatctc ctccctgaaa aactcaaggc aagaaagaga gaaatgacat
    13321 ttcagagctg gctgagactc gccactatat gtgttgtggc tatacttctt ataccatcaa
    13381 tcatcatgct tacagacctc aggaaaatgc aggttttcat aaagagtgaa caggctgatg
    13441 tggcctcaaa aaaagaacag ttaaggctcc tgggaagcat taccacagac tcccgttatg
    13501 gtgagttgat ggcactcaac aaagaagttg gtaagaagga tgttaccttt gtaaccctgt
    13561 tgaaatacct ttcctcacgg ttaccgagag atgtttatat tagagagatt ggatttaccg
    13621 acaaaaataa aaggttcgat tcaactataa aggatttatc aagagagcac tctaccaaag
    13681 aaggatccgg tatcagggag acagctaaga caacacccaa agaagacgct gaggcatcat
    13741 caccatcaaa gggggctatt cctgcgaccg gtcaaagtac tttggcgatc attccaggtg
    13801 agcctgatta tgcagtaacc ttaagaggtt atatttttgg tgacattgac gttctcgaac
    13861 cagccctcgt aaatctggtc atcagcattg agcggtatgc tcttttgagg aacgttgagg
    13921 tgtcgatgag agagacaaaa gagattcgag ggaaaaaggc tcttgagttc attattacgg
    13981 gtcaatgtat acgtcatgag atttaagatc atatacctct ggttcagtct gcccgttctt
    14041 cttgcagcaa catggttttt tgcattctac tcgcctgtat cctctttcat tgagaaacaa
    14101 cgtgcagagc tctcagcagc caagaggaca ggagaaatga tggggaaggc cctcagagat
    14161 gtactggaaa cacgaaaaaa agatgctacc gcacgcctgt cacttgacgg tatatcgaaa
    14221 aacgtgcctg cgtatcagca atttcccaca cttatcaggg ctataaccga atcggggaaa
    14281 aaagaaggga ttgtgttcga tttactgaac agcaatgctc tgccgtatga ctccgggcaa
    14341 gcttccccac tgatgaagcc tgcccttggt gtcgggttta aaggaaagtt tctggatata
    14401 ggtaagtttc ttgaagcagt tgaaaaacaa aaagggttta agcgcatagt ggaaggaaga
    14461 ttatcatatg cagacaagga ttatcccata ctgactggaa atttcctcat tgaatttgtg
    14521 gtatggaaag gggattagtt ttgtgaaagt aagtaaatct atattggttg ctcttatact
    14581 tgcagtgctc tttgcagcat attcgttctt atttacagac aagaaggtgc aatcaacctt
    14641 tcctgttcct gtcacaaaca tagccgggac aactccgcag gccccatact ccacaactgg
    14701 aagcaaaaca ccaaggctca atgtagacac tctaaacctt tcctggcaga atgacccctt
    14761 ttcccttcca aaatcggtta tggaaaaaaa agctgagaag ccaaaaatcg ctctgaagct
    14821 cgtcgcaatt atggaaagca atgcagggcg gtacgcaatt attggtggag aaatagttaa
    14881 aaaaggtgac aaaatcgggg aagagaaggt tgttgaaatc cacaatgaca gagtagtgct
    14941 tattcgtaat aatacgaaaa ggatattatc aattgaagat accaggtagt agaacaattg
    15001 caataataag taatactggg ggataacgtg aaaagactat tcatttttat ttgtattctt
    15061 tttttatgcg cagcgtgtag acacgtgcag acagcgacaa ctccttctgc ccccattgca
    15121 gtcaaacctg ttgaaataat gagtcctgtt gttccccagg tcgaaaaaga aagcaaaact
    15181 ttggctgagt cttatgctga gcgtttctcc tttactttga aagaagctga cgtgaaggat
    15241 gttctccgag gtctttcaaa gcagacgaac tacaatgttg ttgtagaacc tgatgtaaaa
    15301 ggggtgtgca ctgtcgattt aaaagatgtt accctcatga aggctctgga atatatcctt
    15361 gaacccctcg atttcacctt caaaattgaa gaaaagacaa tttatgtatc aaaaccaaag
    15421 ctcgagacaa aatttttctc actgaactat gtggcgctaa aaaagatagg gacaagcagc
    15481 gtaattggct ctgctggcgg agtaggctct ttggggacag caggcagcag cagtgaatac
    15541 cgttccgtgg aaatcaagac agagactgag gctgacatat ggaaaaatat cgaggataac
    15601 ctcaaggtca tgctttccaa agacggtaaa gttgtggtaa ataggcaggc cttgatggtc
    15661 ctggttaccg attatccaaa aaacttgaaa cagattgcct cttttctcaa cgctgtagaa
    15721 ggttcccttc acaagcaggt gatgattgag gcaaaaataa tagaggttca actgaatgat
    15781 gcctcacgag aaggtgtgaa ctggcaattc attgaaggca ggatcggaga gttttttgtc
    15841 aataccaaac aggtatttct caacccaaac attattaacg tcccaagtac aacccaggca
    15901 acagccacaa caccttactt caggcttttt gtgggcaaca aacatctgaa tatagacaac
    15961 acctttattg atctcctcaa aactcaagga aatatcaatg tagtctcaag ccccagaata
    16021 gcaaccctca acaaccagcg ggcagtaatt aaggttgcac ggcaggacgt ctattttgat
    16081 gaacagcaat ccgttggcgt tgtcagtacc acctccctgg caacatatac accaaaattt
    16141 atcacagtag ggctgatgtt ggatgtaacc ccacagatag ataacaacgg gaatattgtt
    16201 ctcaacattc atcctatgtt aacagaaaag gtcgatgtgg cgctctcacc ttctggcgca
    16261 gcagtcccca tactcgatgt tcgtgaggct gatacaatgg taagggttag agaaggggag
    16321 acagttatta tcggtggact catgaaagat cgaaagagaa tttctgatac cggcacgaag
    16381 ggactcatgg atttgcccct cattggctgg cttttcagac tcaaagagtc tgaagcccaa
    16441 aaaaatgagc ttgttgtgtt cctaacaccg agaatcatgt caaccggaga aaccccatga
    16501 gcgtcatcct cgatgccctg aagaaggcac agaaagaaag aaaggatatg acaaaaaatc
    16561 tcccctacaa tcccagggaa aagtcgcaga aatcacgttg ggttttatat accggtgcct
    16621 tgcttgtgtt ctgcgttctc ctcgtatttt tcttgatccc tgacatgaag aaagcaccca
    16681 agataatgat agaagttaac cctgtccaca ctcctcagcc tatatcgcaa cctgttgccc
    16741 ctgaaaagac agcagcagtt gttcagtcaa cagtcgctgt caatccgctt gcctcagagc
    16801 aattagacag gcgtgataac ataacaggac acgtgaaacc tgtgaaaaca caaaaaaaca
    16861 ttgcctcact gctatctaaa aacgcatcgg gcatgacaaa atcccgagtg gagactctgc
    16921 ccgaaaaggt ttggcaccag gatgaggcca gggttcttat ttcatcgaca gattttgaaa
    16981 aaatcaatct caaattccat aaggctgtaa aggagacaga gaggggcaat atctcagaag
    17041 ccaaacagct ctatcatgca atcctggctg agcagccaag caatgttgaa acgcttaaca
    17101 acttaggggt tatctccatc aaggaaggca acactgagga ggctctattt tatttcaata
    17161 aggttttcca gtacaacaag aattatggaa aggcatacaa caatgttggc ctcgtcatga
    17221 tgaaaggtgg tcagaaaggc cttgcagagg agtatttcag aaagtctata gagctgaaaa
    17281 aagatgatgt tgaacccgtt atcaaccttg cagcgctact gcgcacagaa aaaagatatg
    17341 aagaagcggc gaggttgctt gaaaacctcc tcagaaccag cgtgactagc aagcctctct
    17401 acctttcgta tgcactcata aaggatgacc tgggacatta cgaacaggca ataaaatatt
    17461 acaggttata tctcagggga agcatgggag atagccaacg aagcgatgta ataaaaagac
    17521 ttgaagttct tgagaatgca cagtcttcac gaactcatta atggcaattc attgactgct
    17581 gcgataatta taacaatgat gcttggagta cgtgaaccaa atatagagtt aactgacccg
    17641 cctttggacg agaggaaaag gcgacgacgg cagatatgga gaggctaaga agttaggatt
    17701 gttaatatct tgacatttct catctcctta gcttctagct ttaaagaagg ggataacctg
    17761 tttgctgaag cctcggcgca ggcagatacg gatcccggca atagcagcca tagggagcag
    17821 agcaggacat ggcacggatg atacggaaaa aattaggaga gatacttgtc ggtgcaggga
    17881 agttaagtga cgaatccctt gtcagagcct tggatgagca aaagaagtat ggtgaaaaac
    17941 tgggcaagat tcttacaaaa atggaattcc ttacagaaaa ggagattatc gacacactga
    18001 gccaacaact gggaatacct atcgtttccc ttgatggccg cgatattccc atggatctca
    18061 tacggctctt ccccgaagaa gtctggaaaa attacatggt catccctttt gaaaaacatt
    18121 tcaatgtttt gagaatagcc atggtagacc cccttgatat caatgccatc gatgaagttg
    18181 ctcggctcgt caagatggag gttgagccgt gtatagtcac tgagggagac atgaaaaggg
    18241 ccttggccaa gtattatggg gcaaaaacca ttgttgaaca gacccttgaa gatatgggcg
    18301 aggatgttga tctgacactg ataaaagatg caagccttga agaggtagat aaaatatcca
    18361 ttgatatgac cgaacaggag cctgttgtaa ggttcgtcaa cagcgttcta acccagtcgc
    18421 tggcagatgg tgcaagcgat attcatgttg aaccgctgga acgtaagatg cgtgtgcgga
    18481 tgcgaattga tggaaggctc agggagatcc ctgcccctga aaagaagatg tttctcccaa
    18541 taatctcacg cataaagatt cttgcaggca tggatatagc aaagacaaga atacctcagg
    18601 atggccgttt cgatatcagg gaaggggcaa aagaggttgg cgtgagagtg tccacatttc
    18661 ccaccatcca cggggaaaaa gcggtgcttc gccttcttga taagggtgct gcactttatg
    18721 gcattgacaa gttgggcctc cttcctgatg acgaaaagac tttgaaaaat gttgtgaaaa
    18781 ggccctatgg atttatcctt tcaacagggc caacaggaag cggaaaatct accacactct
    18841 atgcaatact caacttcatc aactctcctg aaaaaaatat tattaccatc gaagacccgg
    18901 ttgagtacac aatcgatggt ttggctcaaa ctcaggtaaa tccaaaagcc ggattaacct
    18961 ttgattctgg gttaagagca atcttaagac aagacccgga catcattatg gttggtgaaa
    19021 tccgagacaa ggagacagca acaattgcca ttcattcagc cctcac
//
LOCUS       DAOOMC010000049        19005 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdus sp. isolate AMR_MDS_2685
            NODE_2964_length_19005_cov_5.962322, whole genome shotgun sequence.
ACCESSION   DAOOMC010000049 DAOOMC010000000
VERSION     DAOOMC010000049.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864760
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdus sp. (wastewater metagenome)
  ORGANISM  Syntrophorhabdus sp.
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae; Syntrophorhabdus.
REFERENCE   1  (bases 1 to 19005)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19005)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,729
            Genes (coding)                    :: 2,708
            CDSs (with protein)               :: 2,708
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 7 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19005
                     /organism="Syntrophorhabdus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2964_length_19005_cov_5.962322"
                     /isolate="AMR_MDS_2685"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2699756"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..324
                     /locus_tag="PK125_06560"
     CDS             <1..324
                     /locus_tag="PK125_06560"
                     /inference="COORDINATES: protein motif:HMM:NF024675.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HPB37804.1"
                     /translation="SWFEYLVSIGISLPEWEKTGTYVLIRNVHMDLIERLHYGETVHV
                     ETAIKEVRNAHFILTHKVVKSGKITTRGETKMVCVDGMGKPKRIPQDFKNRLLANSKE
                     NKLET"
     gene            complement(329..1636)
                     /locus_tag="PK125_06565"
     CDS             complement(329..1636)
                     /locus_tag="PK125_06565"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HPB37805.1"
                     /translation="MNKKIKVALSILFVSIFILGFLIGAQGKRTISSGDDREIYHYLR
                     TFSDVIDIVKKNYVEEVKDKDVVYAAIKGILESLDAHSSFLPPEMYKEMQSETKGEFG
                     GIGIEITIKDGFPTVITPIEDTPAYKEGLKAGDHIIKIDGKPTKNMSLVDVVKMIRGA
                     KGKPVTLTVVREGFTAPKDFRVVRDIIVVKSVKYRMLEDDYGYIRIVQFQERTAKDLE
                     TAFKELVKSNKDKPVKGIILDLRNDPGGLLEQAVEVSDKFIEEGLIVYIEGRKKDDKA
                     MKFYARKNNDYLGPLVVLVNEGSASASEIVAGALQDYKRAIVVGTKTFGKGSVQTVFP
                     LGDGSAVRLTTAKYYTPKGRSIQAEGITPDIMVDNNIVRGKDKFTPIKEKDLSKHIEP
                     EKNMKKEDEPKTDKNQDKPSDTDDFQLYMGLQMLKGWEALRGK"
     gene            1717..2772
                     /gene="mnmA"
                     /locus_tag="PK125_06570"
     CDS             1717..2772
                     /gene="mnmA"
                     /locus_tag="PK125_06570"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR00420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HPB37806.1"
                     /translation="MKTVFVAMSGGIDSSFAAYLLKREGFKVIGITFQLLPNHTSTIN
                     NSKTCCSNEAVLRAKKAADTMNIPHYVIDLRKEFEEFVIERFIKEYGAGRTPNPCVLC
                     NQFIKFSAFFYKALALGGDYIATGHYAKIEKMSDTYFLSKATDKSKDQSYFLYSIKKD
                     QLSRILLPLGVYTKSEIIPAIREMGWKNTSMYKESQDLCFIPNGQYRSFLSNFMRLEK
                     GPIFSIKGKLMGHHDGIHLYTIGQRRGLNIPYKEPLYVIEIRPHENCLIVGSKNHLMR
                     KSLTATPTNLLSDISSGITAKVRYRQKEQPCSYSLKDDFMRVEFYECIDAITPGQSVV
                     LYNEDVVVGGGIIKSTE"
     gene            complement(2858..4036)
                     /locus_tag="PK125_06575"
     CDS             complement(2858..4036)
                     /locus_tag="PK125_06575"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HPB37807.1"
                     /translation="MLNLSEKHSMIQKIAEKIAKEKVAPRAKEIDSTGAFPWDLIDVY
                     KKHGFLYLMLPERFGGLDGDITSLCLVTEELAKVSGASSLIPLAHHVGVMPIMVAAND
                     EQKEYLYNKIIDPEKMYIVAFCLTEPQAGSDASHMATSVVKDGNYYYMNGKKSMITNG
                     ANADLYTVFATTNPKARTKGISAFYVERDYPGVIIGKSEDKLGMIGSDITEIMFENVR
                     LTKDNLLGKEGQGWDIAMSTLNLSRPAVGAQAVGIAQGALDFSVEYAWKRVQFGQKLA
                     DFEGIQFMVADMATQIEAARALVYDAAHLLDMKVYERDKMSAIGVDKLSAMAKVYSAD
                     VAMKVTTDAVQILGGCGYTKDYPVERMMRDAKATQIYEGTNQVQRIVIARDIFRKFMP
                     "
     gene            4119..5222
                     /locus_tag="PK125_06580"
     CDS             4119..5222
                     /locus_tag="PK125_06580"
                     /inference="COORDINATES: protein motif:HMM:NF015948.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HPB37808.1"
                     /translation="MPTVYFANMRTTPTRNLLDKLSDLLNRTKINTIIKKDDLVAIKL
                     HFGEQGNTAYLRPLFLRTIVSKIKQLGCKPFLTDTNTLYSGARSDAVSHFNIAIEHGF
                     GYSSVGCPLIIAGGLKGQSGVKIPVEGEVLREAIIAREIVEADCIIAVSHFKAHELTG
                     FGGALKNVGMGCATREGKLIQHSSVAPQVDSGKCKACRLCIGYCAANAITIKKKKALI
                     NSDICTGCGECILICPHKAIDIQWNEPDDIFQKKMVEYAAAALAGKKKKSIFMNFLIQ
                     ISPACDCYSNSDAPIVKDIGILASVDPVAIDAASCDLVNNETSIPNTALKKILKPGED
                     KWRALFPTIDWQYQLDHAENMGLGSRNYTLVKI"
     gene            complement(5484..6059)
                     /locus_tag="PK125_06585"
     CDS             complement(5484..6059)
                     /locus_tag="PK125_06585"
                     /inference="COORDINATES: protein motif:HMM:NF014025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarC family protein"
                     /protein_id="HPB37809.1"
                     /translation="MLSDFWLSFIPLFIAFDVFGVLPLYIRFIHRLDDSRRKRNLRDS
                     LITAFFTALLFVIMGSKVMEYLGISIEDFLIAGGIVLFIIAAKYIIVGQDENLEEDEM
                     YGVVPLGVPLLSGPAVLATSIIIRNQYGFTYFFVSLALNLVICAVVLKFSQYIIKYLG
                     KRFVEALSKVFSLIIASIAIMFIRKGLQGLS"
     gene            complement(6120..6302)
                     /locus_tag="PK125_06590"
     CDS             complement(6120..6302)
                     /locus_tag="PK125_06590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB37810.1"
                     /translation="MKVYVNDQEVDLAPGMNVRHALTAAGLINEIDKGKKVYDEMGNE
                     IGLGGALTDGLRINVR"
     gene            complement(6299..7849)
                     /locus_tag="PK125_06595"
     CDS             complement(6299..7849)
                     /locus_tag="PK125_06595"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0009166 - nucleotide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-nucleotidase C-terminal domain-containing
                     protein"
                     /protein_id="HPB37811.1"
                     /translation="MFLAKKLQHFLIACFFLLSASTFLSCVAEHEIRILHMNDFHGFA
                     MPYKPYGSDEAQGGLAYLASRAENLRAEKPTLFLAAGDMIQGNNWANLFQGKSSIEAM
                     NVMGFDAMVVGNHEFDFGQAVLRERIGEANFPILGANVVGLNELKPYIVKNMDGITVA
                     VIGVVTDDTPVTTHPKNVKGLKFLSTEETIKKYIQELRKKSDIIVILSHAGFSADTEL
                     ARKVDGADIIVGGHSHTKVAKPVPINKSFVVQAFEHGKTLGVVDITLKYGKVVDVSGY
                     LEPIKPTGKQNKAVEAIVTKYQQKVDIVMNETVGEALVDLDGVNVRLQETNLGNLITD
                     IMRRTTGADAAIINGGTIRTSIKKGLVKVSDVYAVVPFDNYIVAIKLTGQQIRDTLEH
                     GVTGVEDEEGRFPQVSGLTFTYDIKGKKGSRVKDIFIGGIPLAAEKEYTVATNDFLAA
                     GGDGYKAFGDSVKSSRDYAVIGGAMRGEKLVYIDSGRWLRDVVIDYIKTHKVISPEIE
                     GRIKEIKG"
     gene            complement(7836..8852)
                     /locus_tag="PK125_06600"
     CDS             complement(7836..8852)
                     /locus_tag="PK125_06600"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HPB37812.1"
                     /translation="MKVIDFTSGSNPLGPSNKARNIIRTRLRQISAINADGLDRLKNY
                     IAKKEGIDDACILFGCGSTVILNTILDHITPKRILIPYPVSKRHRSVFLKHSLECVTV
                     PLKAEENYDLTIKDFCDAMHGCGAALLPNPHDVTGTVISDRDIAKIADNADELGISLL
                     IDEAYSGYASLPSLAVRVSNSKGAAILRTFSTFHALGGLRLGYVIGPPDFINQIRAKL
                     DPSWINTLAPWAAIASMKDKGFQRRTLLFLEGEKVYIQDKLSGTKDMKCSVSPSNILV
                     VRHLKGPGDVQKRFERNKVHMNTFVGEDGNICIPFPIQNHRLNAYFVKVVKKMMGVQK
                     HVSG"
     gene            complement(8936..9685)
                     /locus_tag="PK125_06605"
     CDS             complement(8936..9685)
                     /locus_tag="PK125_06605"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPB37813.1"
                     /translation="MREKEQTAEGKFDMLMKIEGLSVAYGTIRALDDVVLEVMPGEIL
                     SVIGANGAGKSTLLKTITGITKPFAGTIVFNGMAIHGARPDKVVKLGITIVPEGRRIF
                     PDLTVKENLELGGYTITDRALKTELFDLVVTTFPRIKERLKQHAGTLSGGEQQMLAMG
                     RALMGNPKLLLLDEPSMGLSPIITREIFSLMRLINRDRGISMILVEQNAHMALEHSER
                     AYVLENGRVTMNGLSREVKSSPGIIEAYLGV"
     gene            complement(9682..10449)
                     /locus_tag="PK125_06610"
     CDS             complement(9682..10449)
                     /locus_tag="PK125_06610"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPB37814.1"
                     /translation="MNALEVEGLEKSFGGVKAVDGISFVVEEGKTVGIIGPNGAGKTT
                     VFNLITGFYRVDRGRILFYGKNITRTQPYKLPHLGIARTFQNLRLFSNLSVFENVLSA
                     ILKKDGYNVVSSVFRTADYFMKEGKAEKKARELIEFFQLSGKENYPANNLPYGEQRRL
                     EIARALALQPKILLVDEPGAGMNPREVQDLLQTLREVKEQFSLSIVIIEHQMGLVMNI
                     SDKVVVMDFGEKIMEGTPGEVKKDKRVIEAYLGEEFI"
     gene            complement(10495..11433)
                     /locus_tag="PK125_06615"
     CDS             complement(10495..11433)
                     /locus_tag="PK125_06615"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HPB37815.1"
                     /translation="MNFFWEARKVILLSVLIYLSVKAIFFFGYVSQYIEQITLYALIV
                     ILASLGLNVIYGYTGQFSLGHAAFYGIGAYVSAYLTKIFSIENIFAYIPILFISGAAA
                     GLIGYLIGIPILRLRDDFLAIATLGFGTLVRVLLDNSDKFSEVFGGSRGFVGIPRLTT
                     FELAFVIVIFLILITHNLIYSRYGLFWRCIKNDELASASIGINTFSMKLMAFTYGCFL
                     AGIGGALYANLYTFLHPSNFDILKSIDFLIIVIVGGMGSIAGTIYAGLIWVGFIEGLR
                     IVLPAEVVDLRWVFIPLLLIALMMWKPYGLMTKSRN"
     gene            complement(11430..12320)
                     /locus_tag="PK125_06620"
     CDS             complement(11430..12320)
                     /locus_tag="PK125_06620"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HPB37816.1"
                     /translation="MELLGYIFQQCINGLQLGAVYALIALGYTMVYGVLRLINFAHGD
                     IFMLGAFIAYYLLSRFTLPIYVVFVLTMICTALAGYIIEKVAYKPLRNAPKISLLITA
                     VGVSLFLEYFLSLKAVFTPNYIAFPRPFEVEAFDLSLFTITNVQLIIFSITGVSLGLL
                     YLLVYKTKQGTAMRAVSYDKEIASLMGINIDATVSLTFIVGASLAGVGGILYGIAYPQ
                     INVFMGVMPGIKSFIAAVLGGIGIIHGAVLGGFIIGLSEVFVSAFLSSTFRDGVIFVI
                     LFLVLLLKPTGIFGKRIDKV"
     gene            complement(12405..13535)
                     /locus_tag="PK125_06625"
     CDS             complement(12405..13535)
                     /locus_tag="PK125_06625"
                     /inference="COORDINATES: protein motif:HMM:NF024850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HPB37817.1"
                     /translation="MKKMLFICAVLLFSVFYTGFSLAKDVVKIGLITPLTGDVKTFGE
                     STKNAFNIALEEYAKTGKYRIQPIIADDRNDPTEGANAALKLITQDKVAAIIGPLTSK
                     VAIPVSEIANSNKTPMISGTATNPKVTSTDGKRKPYVFRACYTDPFQGTVGANFAIKQ
                     LKAKTAAVLYDVGNDYSKGLAEYFKTTFTKGGGNIIAYESYQKDDVDFSALITKVGIK
                     KPEIIYIPDYYNKVALVAKQIREKGLKSALIGGDGWDSPELIKIAGNAIVGGSFTNHY
                     SPDRKDPVADTFIRKYKEKFGIVPDALGALGYDSTVILLKALDSVKKITQEEIMTALS
                     SVKNYKGVTGTISFDKNGDAIKSAAILKVEKDGFKYLTTINP"
     gene            complement(13763..14800)
                     /gene="selD"
                     /locus_tag="PK125_06630"
     CDS             complement(13763..14800)
                     /gene="selD"
                     /locus_tag="PK125_06630"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015905308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004756 - selenide, water dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0001887 - selenium compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:complement(14756..14758),aa:Sec)
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HPB37818.1"
                     /translation="MAEVELTQHVRGAGUASKIGPGDLANILCGVEIPQDENVLVGIE
                     GFEDAGVYKISDTVAIVQTIDFFTPIVNDPYSFGQIAAANALSDIYAMGATPKTALNM
                     VCFSPKKFGLEVLRDIIRGGVDKIKEAGVSLLGGHSVEDEEIKYGLAITGIVHPDRVI
                     LNEGARSEDVLILTKPLGTGILNTGIKGNMIGKDTIQRLIYVMAYLNKKASEVMLSVR
                     THAATDVTGFGLAGHLKEMIKENIGVQLFPHKLPYFKEATEMARSGFVPGGLYRNRDF
                     YRGNVVTHEQGFFYDIIFDPQTSGGLLMAIDPEDIRLFEKKAAEVNLEFWVIGKFIDE
                     PKGKILLRDKI"
     gene            complement(14911..15264)
                     /locus_tag="PK125_06635"
     CDS             complement(14911..15264)
                     /locus_tag="PK125_06635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB37819.1"
                     /translation="MVATDYSLYLAVGDRVFHKKFLRWGMGVVVEERRSQLPGGFCYV
                     RINFQDGNIRVFDNNFKSNGCCYYAGIQKIDTEKEMRRSRKKQEALRKKIIKMLLKTR
                     KMLKAPGEDGACGSQ"
     gene            complement(15422..17029)
                     /locus_tag="PK125_06640"
     CDS             complement(15422..17029)
                     /locus_tag="PK125_06640"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HPB37820.1"
                     /translation="MRYGRWMTAKDVLRVNAFKWPKKIGIKDLYKSYTFKEWDERACR
                     LANALADMGMKKGDRFAVLAYNCVEWMEIYAAAAKGGFICVPLMFRLAPAEMEYNINH
                     SECKVFIVQGGVDKADGKEFPWPKTVTEMKKNIPTVTGYMVFGHDNPKVDGFVSYEEA
                     LAKASPEEPATVVTADDPWVIMYTGGTTGKPKGVVKTHLSLFAQYFIMIFDHQFNFDD
                     TNLLVMPCCHVNSLFYSFVATWVGGTVMTYNMVSFNPEDLIKTFAEHKVTFTSLVPTH
                     YIMMLALPDDVKKKYDLSCVKKLLISSAPARRDTKLGVLKMFPNSELYEAYGSTEAGI
                     VTVLKPNEQMTKLGSCGREVIGSDLIRFYDEDGKLVTEPNQVGELYSRSPMLFEEYWK
                     DPEKTAETMKGEYFSAGDMGYRDEDGYVILVDRKANMIISGGENIFPSEVENCVGGNP
                     KVKDVAVIGVPHEKWGEQVTAVIVLHEGQTATPEEITAFCKGKIAGFKVPKNIIFIKD
                     EQMPRSGAGKILHRMLREQYGKWADHQ"
     gene            complement(17088..18356)
                     /locus_tag="PK125_06645"
     CDS             complement(17088..18356)
                     /locus_tag="PK125_06645"
                     /inference="COORDINATES: protein motif:HMM:NF014774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HPB37821.1"
                     /translation="MEDLKELKKIEQYADQCMKCGFCSFFCPVYQEEKEETAVARGKN
                     YLTKLVLKGEQEFTPKMGEIIGKCLLCKRCVAMCPAKTQIDRVVVGARAQMVRNKGLP
                     FIKNFAFRKVMANRKAFGRYVKLASKFQWLMPKTEGKVRHLPDFLKALGQGRNIPEIA
                     KVFLRDKVKRVNKPQNGKEPKMRVGYFMGCATDFIYPELGEKVIDFLTKRGIEVIVPE
                     EQSCCGAAIYFSGDFETGRMLAEQNIKAFQDVDYIVTACGTCSSTLKDYQKYLPDNED
                     QKKRYEAFEKKIKDMTEFVIDVLKIPPEEFKLKKEFEGKTATWHDPCHLIRYQNIKDQ
                     PRAILKALKGLKYVEMPNADLCCGMGGSFSVYHYDITKKIADKKMDGIKATGADIVVT
                     ACPGCMINLIDNTLRSKMPQKVYHLLELVE"
     gene            complement(18375..>19005)
                     /locus_tag="PK125_06650"
     CDS             complement(18375..>19005)
                     /locus_tag="PK125_06650"
                     /inference="COORDINATES: protein motif:HMM:NF014910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HPB37822.1"
                     /translation="ELLDNWVINRIEEMMPMGLPKDADAVLLFEVDGIPEAVEKETQK
                     IVEIAKKYGATDVRVAPDQAEADKYWTARKAGFAAVFGKAKTVFAEDVTVPRGQIPAL
                     INKCKELAKKYNVEIVVLGHAGDGNLHPAILTDINNKEHYDRAVKAMDEIIEGAVDLG
                     GVLSGEHGIGLEKQRFLKRQLEPAVIDMMKKIKALFDPNNIMNPGKIWE"
BASE COUNT         5302 a         4500 c         3615 g         5588 t
ORIGIN      
        1 tcctggttcg aatatttagt ctctataggc atttcacttc ctgaatggga aaagaccggt
       61 acctatgtcc ttatacggaa tgtccatatg gacctcatcg aaagattgca ctacggagag
      121 actgttcacg ttgagacagc cataaaggag gtgagaaacg cccacttcat ccttacccat
      181 aaagtagtta aaagtggtaa aatcacaacc agaggcgaga ctaaaatggt ttgtgtggat
      241 ggaatgggca aaccaaaaag aattcctcaa gatttcaaaa acaggttgtt agccaatagt
      301 aaagagaaca agctggaaac ttaagattct actttcctcg caatgcctcc caacctttga
      361 gcatttgaag acccatgtaa agttgaaaat catccgtgtc agaaggtttg tcttgattct
      421 tatctgtttt cggctcatct tcttttttca tattcttttc tggctcaata tgttttgata
      481 gatccttctc cttaatcgga gtaaatttgt cttttcctct aactatgttg ttgtcaacca
      541 taatatccgg ggttattccc tctgcctgta ttgatctgcc tttcggtgtg tagtacttgg
      601 cagttgtaag cctcaccgca gatccatcac ccaacgggaa tacagtctga acagaccctt
      661 tcccaaaggt ttttgtgccg acaactatag cccgcttata atcctgcagc gcgccagcaa
      721 caatctcaga tgcacttgca cttccctcat taacaagaac aacaagcggc cctaaataat
      781 cattgttttt tcgagcataa aatttcatcg ctttgtcatc ctttttacgc ccttcaatgt
      841 agacaatgag accttcctca ataaacttgt ctgaaacctc aactgcctgc tcaagcaggc
      901 caccggggtc atttctcagg tcgaggataa taccttttac aggcttgtcc ttattgcttt
      961 ttacgagttc cttgaaggcg gtttcaaggt ctttcgcagt tctttcctga aattgaacta
     1021 tacggatata cccataatca tcttcaagca tcctgtactt cacgcttttg acaacaatta
     1081 tatccctgac aacacggaag tcttttgggg ctgtgaagcc ttccctaaca actgttaatg
     1141 taaccggctt accttttgcc ccgcggatca ttttgacaac atctactagg ctcatgttct
     1201 ttgtgggctt accgtcaatt tttataatat gatctcctgc tttcaaacct tctttgtatg
     1261 ccggtgtatc ttcgatgggt gttatgactg ttggaaatcc gtcttttatg gtaatctcaa
     1321 tcccaattcc cccaaactct cccttcgtct cactctgcat ctctttgtac atctcaggag
     1381 gaagaaagga tgagtgtgca tccagggatt cgagaatgcc ttttatggca gcatatacaa
     1441 cgtctttatc tttgacttcc tcaacataat tcttttttac tatatctata acgtcagaga
     1501 aagtccttag ataatgatat atctcgcgat catccccgct ggaaattgtc cgtttacctt
     1561 gagctcctat aagaaatcca agaataaaaa ttgaaacaaa taaaatgctc aaggcgactt
     1621 ttattttctt gttcatcaaa atttcctcct aaagtacaga atactataat tctcgctaac
     1681 tttcaattcg attcatttct atgctaatct tatgccatga aaacagtgtt tgtagctatg
     1741 agtggcggca tcgacagttc cttcgctgct taccttttaa aaagggaagg tttcaaagtt
     1801 attggtataa catttcagct tttgccaaac catacaagca caataaataa ctcaaaaaca
     1861 tgctgctcaa acgaagcagt cctaagggca aaaaaggcag cagacacaat gaacatacct
     1921 cattatgtta ttgacctgcg aaaggagttc gaagaattcg ttattgagag atttataaaa
     1981 gaatacgggg caggaagaac cccgaacccc tgtgttctct gcaatcagtt catcaagttt
     2041 tctgcttttt tttacaaggc tttggcgctt gggggagact acatagccac cggtcattat
     2101 gcgaaaattg agaaaatgtc cgacacctat tttcttagca aggcaactga taaatcaaag
     2161 gatcaatcat acttccttta ctcaataaag aaagaccagt tgagcaggat tcttctgcct
     2221 cttggtgtgt acacaaaatc agagataatc ccggctatca gggaaatggg ctggaagaat
     2281 acaagcatgt ataaggaaag tcaggactta tgttttatac caaatgggca atatcgttcc
     2341 tttctctcaa attttatgcg cttagaaaag gggcccattt tttcgattaa aggaaagctc
     2401 atgggccatc atgacggaat ccacctttac acgatagggc agagaagagg attaaatata
     2461 ccatataaag aacctcttta tgtcatagaa atacggcccc atgaaaattg tctgatcgtg
     2521 ggctcaaaaa atcatctcat gagaaaaagc cttaccgcca cacctacaaa ccttctttcg
     2581 gatatttctt ccggtataac tgcaaaagtc agatacagac aaaaagaaca gccatgttct
     2641 tattcactaa aagacgattt tatgagggtt gagttctatg aatgcattga tgcaattaca
     2701 cccggacaat ctgttgtatt gtataatgaa gatgtagttg tcggcggtgg aattattaaa
     2761 agcactgagt aaaacagtat tcgactttta aaagtcagct atattaaagt cctccttttt
     2821 gtcacgctaa ccgcctcaca tccgagttaa aagcttccta tggcataaat ttacggaaaa
     2881 tatccctggc aattactatt ctctggacct gatttgttcc ttcatatatt tgcgttgcct
     2941 ttgcatcacg catcattctt tcaacggggt aatctttcgt atatccgcaa cctccaagga
     3001 tctgtaccgc atcagtggtt accttcatgg ccacatcagc cgaataaacc tttgccatag
     3061 cagataattt gtcgacacct atggcactca tcttgtctct ttcatatact ttcatatcga
     3121 gcaggtgagc ggcatcatat acgagggccc gtgcagcctc tatttgggtt gccatgtctg
     3181 caaccatgaa ttggattcct tcaaaatcag caagtttttg gccaaattga acccttttcc
     3241 atgcatattc tacggaaaaa tcgagggcac cctgggctat accaacagct tgagcgccaa
     3301 ctgccggtcg tgaaagatta agtgtggaca tggcaatgtc ccatccctgt ccttccttgc
     3361 cgagcagatt gtctttcgtt aaacggacat tctcaaacat tatctctgtt atatctgagc
     3421 ctatcatgcc aagtttatcc tcactctttc ctataattac cccgggataa tctcgctcga
     3481 cataaaacgc agatatacct ttggtccttg cttttgggtt tgttgtagca aacactgtat
     3541 agagatctgc gttcgcacca ttcgtaatca tagacttctt gccattcata taatagtaat
     3601 tcccatcctt tacaacgctc gtggccatat gagatgcgtc tgatcctgcc tgtggctccg
     3661 tgagacaaaa ggcaacaata tacatctttt caggatcaat aatcttatta taaagatatt
     3721 ccttttgttc atcatttgcg gctaccatga tcggcataac gcccacgtga tgagccagag
     3781 gaatgagcga tgatgcacct gaaaccttgg caagctcttc agtaaccagg cagagggatg
     3841 taatatcccc atcaaggccg ccaaagcgtt caggaagcat gagatacaaa aacccatgtt
     3901 tcttatagac atcaatcaga tcccacggga atgcaccagt tgaatcaatc tcttttgctc
     3961 ttggagcgac tttttcttta gctatttttt cagcaatctt ctgaatcatg ctgtgttttt
     4021 cagatagatt taacatacta accaacctcc tttatcttga caaaaccgca ctatttttaa
     4081 catacttttc cagtaaatta aataaaaaag gagctgatat gccgacggtt tactttgcaa
     4141 atatgcgtac aacgcccacc agaaaccttc ttgataaact atcagatctt ctcaaccgca
     4201 caaaaatcaa tacaatcatt aaaaaggatg acctagttgc cataaagctt cactttggcg
     4261 aacagggaaa taccgcttat ctgagaccgc tttttctacg aacaatcgtt agcaaaatta
     4321 aacagctcgg ctgcaaacca tttcttactg atacgaacac cctctactct ggggctcgaa
     4381 gtgatgctgt cagtcatttc aacatcgcca tagaacatgg tttcggttat tcgagtgttg
     4441 gatgcccctt gattattgca ggtggactga aaggccagag cggcgtgaag attccagtag
     4501 agggtgaggt tctccgagaa gctattatag cccgggaaat agtcgaggct gactgcatta
     4561 ttgctgttag tcactttaaa gcccatgaac ttacaggatt tggcggtgca ttgaaaaatg
     4621 taggcatggg atgtgcaaca cgtgagggta agctcattca acacagttcg gtagcaccac
     4681 aagttgacag cgggaaatgc aaagcctgca ggctctgtat tggttattgt gctgcaaatg
     4741 caataacaat aaaaaagaaa aaggctctta taaacagtga tatctgcaca ggttgtggag
     4801 aatgcattct catttgtcca cacaaagcca ttgacatcca atggaatgag ccggatgata
     4861 tctttcaaaa aaaaatggtt gagtatgcag ctgctgcatt ggcaggaaag aaaaagaaat
     4921 cgatttttat gaattttctt attcagatca gtccggcttg cgactgttat tccaacagtg
     4981 atgccccgat tgtgaaggat attggtatac tcgcttctgt tgatcccgtt gcaattgatg
     5041 cagcctcctg tgacctggta aacaacgaaa catcgatccc gaataccgcc ctaaaaaaga
     5101 tcttgaagcc cggggaagat aaatggcgag cactttttcc cacaatcgat tggcagtatc
     5161 agcttgatca tgccgaaaat atgggtttag gctcgcgaaa ctataccctc gttaaaattt
     5221 aggctatttt gaacacggtt tttttgattg actggcagat aggtcatcaa ggaacaaatt
     5281 gggcgtcttc atctggcata cattatgtcg ctgagaggag atgctgggtt ttcacttcca
     5341 tttttgatgt atttttccac ctgctgaaac gactagaatt gcgacaaagc gattggtaaa
     5401 gattggaata gcgaattcaa ggcacactat catgtattta cagattattt caggggaatt
     5461 atcatgctag gtgctctata tatctaagat aacccttgca aaccctttct gatgaacatg
     5521 atggcgatac tggcaataat gaggctgaat acctttgata aggcctcgac aaacctttta
     5581 cccagatact tgattatata ttgggaaaac ttgagtacaa ctgcacagat cacgaggttc
     5641 aacgccaggg aaacgaaaaa atatgtgaag ccatattggt tccgaataat aattgaagtt
     5701 gcgagtactg ccgggccaga aaggaggggc acaccaagag gcaccacacc gtacatctcg
     5761 tcctcttcca gattttcatc ttggcccaca atgatatact tggccgcaat aataaagagc
     5821 acgattcctc cggcaatgag aaaatcctct attgaaatac caagatactc catgacctta
     5881 ctacccatga taacaaacaa aagtgcggtg aaaaatgctg taattaggga atcacggaga
     5941 ttcctcttcc gccttgagtc atcaagcctg tggataaacc tgatgtagag cggcagaaca
     6001 ccaaagacgt caaacgctat gaaaagtgga atgaatgaaa gccagaaatc actcagcata
     6061 gaatatcttc cgttttctaa ccttgaacag tgaatctgga gccatgaagg acctactgtt
     6121 catctgacgt taatcctcaa tccgtctgtc aacgcaccgc ctaaaccaat ctcattgccc
     6181 atttcatcat agaccttttt ccccttatca atctcattaa tcagaccagc agcagtcagg
     6241 gcatgtctca cattcatccc tggtgctaaa tcaacctctt ggtcattcac atatactttc
     6301 atcccttaat ctcctttatc cttccctcta tttcaggaga gatgaccttg tgtgtcttaa
     6361 tgtagtcgat gaccacatcg cgcaaccatc tgcctgagtc aatataaacg agcttttcac
     6421 ctctcattgc gccaccgata accgcataat cccttgatga cttcactgaa tcgccgaatg
     6481 ccttatatcc atcaccgccg gctgcaagaa aatcatttgt agctacagta tactcttttt
     6541 cggctgccag aggaattcca ccgataaaga tatcttttac gcgtgaacct tttttgccct
     6601 taatgtcata ggtgaatgta agaccggaaa cctgggggaa cctcccctct tcatcttcaa
     6661 cccctgtcac gccatgctca agggtatcac gaatctgctg tcctgttagc tttatagcaa
     6721 cgatgtaatt gtcaaaagga acaactgcat agacatcgct caccttaact aggcccttct
     6781 taatactggt ccgaatagta ccaccattta ttattgccgc atcagcaccg gttgttcttc
     6841 tcattatatc tgtgatgaga ttacccaggt ttgtttcctg taacctcaca ttaaccccgt
     6901 cgaggtcgac aagtgcttct cccactgtct cattcattac gatatcgacc ttctgctggt
     6961 attttgtaac aatcgcttct actgctttat tctgttttcc agttggtttt atcggttcaa
     7021 ggtaaccact gacgtcaaca actttaccgt atttgagagt aatatccaca acaccgaggg
     7081 tcttgccatg ctcaaaggct tgcaccacga atgacttgtt gatgggtacc ggtttagcga
     7141 ctttcgtatg tgaatgcccg ccaacaatta tgtctgcacc atctaccttc ctcgccagtt
     7201 ccgtatcggc actgaaccct gcatgggata aaataacaat gatatcgctt tttttcctca
     7261 gctcttgtat atatttcttt attgtttctt cggtagacag aaatttcagt ccttttacat
     7321 ttttaggatg ggtcgtcaca ggcgtatcat ctgtcacaac ccctataaca gccacagtaa
     7381 taccgtccat atttttcact atatatggct tcaactcatt taacccaaca acatttgcac
     7441 ccagaatagg aaagtttgcc tctcctatcc gctctctgag aactgcctgg ccaaaatcaa
     7501 attcatggtt ccccacaacc atggcatcaa agcccatgac attcatcgcc tcaatggagg
     7561 atttgccctg gaagagattt gcccaattgt ttccctggat catatctcca gcagcaagga
     7621 atagtgtagg cttttcagct cggagattct ccgcccgtga cgcaagatat gccagtccac
     7681 cctgggcttc atcagaacca tatggcttat agggcattgc aaatccatgg aagtcattca
     7741 tgtggagaat cctgatttca tgttcagcaa cgcaggatag aaaggtactg gctgacaaaa
     7801 gaaaaaaaca ggctatgaga aaatgctgta actttttagc cagaaacatg tttttgcacc
     7861 cccatcattt ttttaacaac ttttacgaaa taagcgttta accggtgatt ctggattgga
     7921 aaaggtatac agatatttcc atcttcaccc acaaaggtgt tcatgtgcac tttgttcctt
     7981 tcaaaccttt tttgcacatc accaggcccc tttagatgcc tcactaccaa aatattcgat
     8041 ggactcacag aacatttcat gtccttagta ccagatagct tatcctggat atacactttc
     8101 tcaccttcca aaaagagcaa ggttctacgc tgaaaacctt tgtctttcat agacgcaatt
     8161 gctgcccatg gcgctaatgt atttatccaa gaagggtcaa gtttagctcg aatctgattt
     8221 atgaaatctg gcggcccgat aacgtatcct aaacgcaaac cgccgagtgc gtggaatgtt
     8281 gaaaatgttc taagtatcgc cgctcctttt gaatttgaaa cgcgaactgc cagggatggc
     8341 agggacgcat acccgctgta tgcttcatct attaaaagac ttataccaag ctcgtccgca
     8401 ttgtcggcaa tttttgcaat atctctgtct gaaatcactg ttccagtaac atcgtgagga
     8461 tttggtaaaa gtgctgcacc gcatccatgc atggcatcac aaaaatcttt aatggtgaga
     8521 tcataatttt cttctgcttt taaaggaact gtcacacatt cgaggctatg tttcaaaaag
     8581 actgatctgt gccttttgga aacaggatag ggaataagta ttctcttcgg agtaatatga
     8641 tcaaggattg tgttcaatat gactgttgat ccgcatccaa acaagatgca agcatcatcg
     8701 atcccttctt tctttgcaat atagttcttt aaacggtcaa ggccatccgc gtttatagct
     8761 gatatctgac gcaaacgggt acgaatgata tttctggcct tatttgaagg ccccaaaggg
     8821 tttgaccctg aagtaaaatc gattaccttc accgaggatt ccccgcggta gttgtaggct
     8881 gtagactgat ggccgaaaag aaaggttttc tataaatcct tatgagcttt atcatttaca
     8941 ctcccaggta tgcttcaatg atgccaggac tggatttgac ttctcttgac agcccattca
     9001 tagttaccct tccattctca agaacatagg ctctttctga gtgttccagg gccatatggg
     9061 cgttctgttc tacgagaatc atagatattc ctctgtctct gtttataagt cgcatgagcg
     9121 aaaatatttc tcttgttata atgggggata aacccataga aggctcgtca agaagcaaaa
     9181 gtttgggatt tcccataagc gctcttccca ttgcaagcat ttgctgttcg ccacccgaaa
     9241 gagtccctgc atgctgcttc aacctttcct tgattctcgg gaaggttgtc acaacgaggt
     9301 cgaagagttc agttttcagg gcacgatcag taatagtata gcctccaagt tccaggtttt
     9361 ctttgacagt aagatcagga aaaatgcgtc tgccttcagg aactatagta attccgagct
     9421 ttacgacctt atcaggtcgc gctccgtgta tggccattcc attaaacacg attgtacctg
     9481 caaaaggttt tgtaatacct gtgatcgttt tcaatagggt cgatttcccg gcaccatttg
     9541 cccctatcac cgaaaggatt tctcccggca tcacttcaag gactacatca tcgagcgccc
     9601 gaatagtccc ataagccact gaaagccctt caatcttcat caacatgtca aatttaccct
     9661 ctgccgtctg ctctttttcc ctcaaataaa ctcttccccc aggtacgctt caatcactcg
     9721 tttatctttc ttcacctctc ccggcgtgcc ttccataatc ttttctccga aatccataac
     9781 cacaacctta tcagagatat tcataacaag acccatctgg tgctctataa tgacaataga
     9841 aagtgagaat tgctccttca cttctctcag agtttgaagc aaatcctgca cctccctggg
     9901 gttcataccc gcccctggtt cgtcaacaag aaggattttt ggttgtaatg caagggcacg
     9961 ggcaatctca agtctccttt gctcaccata gggaaggttg tttgctggat agttttcttt
    10021 tccggatagc tggaaaaact cgataagctc tcttgctttt ttctctgcct ttccttcttt
    10081 catgaaataa tcggcggttc tgaaaacaga agatacaaca ttataaccat ccttcttaag
    10141 aattgccgat agcacattct caaataccga gaggttcgaa aagagtctca ggttctggaa
    10201 tgtcctggca attcccaagt gcgggagctt gtaaggctga gttcttgtaa tattcttacc
    10261 atagaaaagt attctccccc tatcaacgcg ataaaaaccg gtgataaggt tgaagaccgt
    10321 ggtcttacca gcgccgttgg gtccaatgat gcccactgtt tttccttctt caacgacaaa
    10381 ggatattcca tcaacagcct ttaccccacc aaagcttttc tcgagcccct caacttccag
    10441 tgcgttcatt tttcactaat gcctttctgt tcgataacac agcatctccg cttctcaatt
    10501 tctgcttttt gtcatcaatc catatggttt ccacatcata agggcaatga gcaataaggg
    10561 gatgaaaacc cacctaagat ctactacctc tgctggaagc actatacgta agccttcaat
    10621 gaaccctacc cagatcaatc cggcatagat agtccctgca atactgccca taccaccaac
    10681 aattacgatg ataagaaaat cgattgattt caaaatatca aaatttgacg ggtggagaaa
    10741 ggtgtaaaga tttgcgtaaa gagccccacc aattccagca agaaagcaac cgtatgtaaa
    10801 agccatgagt ttcatggaaa aggtgtttat ccctatggaa gcagatgcga gctcatcgtt
    10861 ttttatacat ctccaaaaaa gaccatatcg ggaataaatt agattatgcg taataagaat
    10921 gagaaaaatc acaataacaa aagcaagctc aaaagtagtc agtcgtggga taccgacgaa
    10981 acctcgtgag ccgccgaaaa cctctgagaa cttgtccgag ttatccagaa gaacccttac
    11041 aagggttcca aaaccaaggg ttgcaatggc aagaaaatca tcgcggagcc tcaggattgg
    11101 tatgccgatc aaatagccta ttaaccctgc tgctgcacca cttataaaaa gaatcgggat
    11161 ataggcaaaa atgttttcta tgctgaatat tttggtcagg tacgctgaga cataagcacc
    11221 aatgccgtaa aatgcagcgt ggcctaatga aaactggcct gtatagccat agataacatt
    11281 caaaccaagg gacgcgagga taacgattaa ggcatagagt gtgatctgtt cgatgtactg
    11341 tgatacataa ccaaagaaaa aaatagcctt cacagaaaga taaataagga ctgataacag
    11401 gataaccttg cgggcttccc agaaaaaatt cataccttgt ctatcctttt tccgaaaatg
    11461 cctgtaggct ttaatagcaa cacaaggaag aggataacaa aaatgacgcc atccctgaac
    11521 gtggaagata aaaatgcaga aacaaaaacc tccgacaggc cgataataaa ccctccaagc
    11581 acagccccgt gaatgatccc tattccaccc agcactgctg caataaaaga cttgatgccc
    11641 ggcatgactc ccatgaaaac gttaatctgt gggtatgcga tcccgtaaag tattccaccc
    11701 actccagcca acgaagctcc gacaataaat gtaagggaaa cagtagcatc aatgttgata
    11761 cccatgagag aagcaatctc cttatcatag gagaccgctc tcattgcagt tccctgtttt
    11821 gttttataaa cgagcagata aagcagcccc agagaaactc ccgttatact aaaaatgatc
    11881 aattgcacat tggtaattgt aaagagtgac aaatcaaagg cctccacctc aaagggcctc
    11941 gggaatgcga tgtaatttgg tgtaaaaaca gctttcaggc ttaaaaaata ttcaagaaaa
    12001 agtgatacgc caacagcagt tatgagaagt gagattttgg gtgcattcct taagggcttg
    12061 taagcaacct tttcaataat gtacccagca agggcagtac aaatcattgt caggacaaat
    12121 acaacataaa ttggcagagt aaacctggaa agcaggtaat aggcaataaa cgcacctagc
    12181 atgaaaatat caccatgagc aaagttaata agcctcagta ctccgtatac catcgtatag
    12241 ccgagtgcta taagtgcata aacagcccca agctggaggc cgtttatgca ttgctggaat
    12301 atgtacccta atagttccac aactgggata tctgtggtcc gaggatcaag gttaaaggtt
    12361 caaggtttta aacttagctc cgtaccctaa cctctcgcct ctatttatgg atttatggtt
    12421 gtcaaatact tgaatccatc cttttcaact ttaaggatag cggcagattt aatagcatct
    12481 ccgttcttgt caaaactgat ggtgcctgta acgcctttat agtttttcac agaagaaagg
    12541 gctgtcataa tttcttcctg tgttattttt tttacactat cgagtgcttt cagaagtatt
    12601 actgttgaat catatccaag tgctccaagg gcgtcgggaa caataccaaa tttctcctta
    12661 tattttcgaa taaaggtgtc tgcaacagga tccttcctgt caggagagta atgattggta
    12721 aaagaaccac ccacaattgc attgcctgca atctttataa gttccggaga atcccaccca
    12781 tccccaccga tcaaagcact ttttaagccc ttctccctga tttgtttcgc aacaagagcc
    12841 actttgttat aataatcagg gatatatata atctctggtt tctttattcc cactttcgta
    12901 ataagggctg agaaatcgac atcatctttc tgataggact cataggcaat aatgtttcca
    12961 ccaccttttg taaatgtcgt cttgaaatac tctgcaagtc ccttggaata gtcatttcca
    13021 acgtcataca agacagcagc tgttttcgcc ttcagttgct ttatagcaaa gttcgctcca
    13081 actgtgccct ggaacgggtc tgtatagcat gctctgaaaa catagggttt tctttttcca
    13141 tcagtggagg taactttcgg attcgttgca gtgcccgaga tcatgggggt tttgtttgag
    13201 tttgcaatct cgctgactgg tatagctacc tttgatgtga gaggaccaat gatggcggca
    13261 actttgtcct gagtaatgag tttcagtgct gcgtttgcac cttcagtagg atcattcctg
    13321 tcgtcagcta tgatcggttg aatcctgtat tttcccgttt ttgcatattc ctcaagagca
    13381 atgttaaagg cattctttgt tgattcacca aaagtcttaa catctccagt caatggggta
    13441 ataaggccga ttttcacaac atccttcgcg agagaaaatc cggtgtagaa cacagaaaag
    13501 agaagtacag cacagatgaa cagcatcttt ttcatgaagc cagtcctcct tgtgattgca
    13561 ttatataata aaaaacatac cctacaactg taaagcaacc tttttgtggg tgctgaaacc
    13621 aatctactcg ctggtaagtg gattaaaact taaattagaa gaaaacagac ggtaggctaa
    13681 agcattggag cattatggga tcagatgatg aggcaaatta aatgaatggc tttcctgtta
    13741 ctcattgccc gtcaccaaga cctcatattt tatcacgcaa taggatttta ccctttggtt
    13801 catcaataaa tttaccgatt acccagaatt cgaggttgac ttcagcagcc ttcttctcaa
    13861 ataaccggat atcctcagga tcaattgcca taagcaggcc gccggaagtc tggggatcaa
    13921 agatgatgtc gtagaaaaac ccttgttcgt gggtcacaac atttcccctg taaaaatcac
    13981 ggtttctgta caggccacca ggaacaaaac cagacctggc catctcagta gcttctttaa
    14041 aatatggcaa tttatgggga aaaagctgta cacctatatt ttctttaatc atttccttca
    14101 agtgaccggc cagaccaaac cctgtgacat cggttgccgc atgagttctt actgaaagca
    14161 tcacttctga agcttttttg ttcaagtagg ccatcacgta gatgagcctt tgtatggtat
    14221 cttttccgat catgtttcct tttataccgg tgttaagaat acctgtcccg aggggttttg
    14281 tcaggataag gacatcttcc gatctggccc cctcattgag gatgaccctg tcaggatgaa
    14341 caatcccggt aattgcaagt ccatatttaa tctcctcatc ttccacgcta tgaccaccaa
    14401 ggaggcttac acctgcttct tttatcttgt caacgccacc tctgatgata tctctcagca
    14461 cttccagacc aaacttcttc ggtgaaaagc aaaccatatt gagtgctgtt ttcggtgtag
    14521 ctcccatggc ataaatgtcg ctcaatgcgt ttgctgctgc aatctgacca aaggaatagg
    14581 gatcgtttac aataggggta aagaaatcaa ttgtctggac aattgccacc gtatctgaaa
    14641 ttttgtatac acctgcatcc tcaaatcctt caatacctac aaggacattt tcatcttgtg
    14701 gaatctcaac cccacataaa atatttgcca ggtcacccgg acctattttg gaagctcatc
    14761 cagccccgcg cacatgttgc gttaattcga cttccgccat gtattctccc ttttaaattc
    14821 aatactcagt tcactgtttg ttctattacc cttaacagat caggggttac acgctttatg
    14881 attttcttat cacctattgc ccattgcgaa ctattggctt ccacaggccc catcctctcc
    14941 tggagccttc aacattttac gtgtcttgag caacatcttg atgatttttt ttcggagggc
    15001 ttcttgtttc tttcttgacc ttctcatttc cttttcagta tctatctttt ggataccagc
    15061 gtaatagcag cagccattgc ttttgaagtt gttatcgaaa acacgtatgt tcccgtcctg
    15121 aaaattgata cgaacatagc agaaaccacc ggggagttgg gatcttctct cttcaacaac
    15181 cacacccatg ccccatcgca aaaatttctt gtgaaaaaca cgatctccga cggccaaata
    15241 aagagagtaa tctgtcgcta ccatggccta ttatatagca tatatcctgg tgtgtgacaa
    15301 ccgggtaagg ggttagaagt tctgtttata tattgcatga ttggattgat cggtgaattg
    15361 taatcaaatt gaaacaaaaa acgggggcaa ttgcccccgt tttacttcag ttgccagtat
    15421 tttactgatg gtcagcccat tttccgtact gctccctcaa cattctatgg aggattttac
    15481 ctgcaccact tcttggcatc tgttcatcct taatgaaaat aatgttcttt ggaaccttga
    15541 aaccagcgat cttgcctttg cagaatgcgg tgatttcctc gggtgttgct gtttgtccct
    15601 catgaagaac aataactgct gtcacctgtt cgccccattt ctcatgaggt acgccaataa
    15661 ctgctacatc cttaaccttt ggatttccgc cgacacagtt ctcaacctct gatggaaaga
    15721 tattttcgcc accggagata atcatatttg cctttctgtc aacaaggata acatatccat
    15781 cctcatccct atatcccata tcacctgcgc tgaagtattc acctttcatt gtttcagcag
    15841 tcttctccgg atctttccag tattcctcga aaagcatcgg gcttcttgag taaagctcgc
    15901 ccacctggtt gggctctgta acaagctttc cgtcttcatc atagaatctg atcaggtccg
    15961 aaccgattac ttcgcgaccg catgaaccaa gtttcgtcat ctgctcattc ggtttgagca
    16021 ctgtcacgat gccggcttcg gtagagccat aggcttcata cagctctgag ttggggaaca
    16081 ttttcaaaac accgagcttc gtatctcttc ttgcaggtgc agaagagatg agaagcttct
    16141 ttacacagct aagatcatat ttcttcttca catcatcagg cagagcaagc atcataatat
    16201 agtgagtcgg cacaagtgat gtaaaggtga ctttgtgctc ggcaaatgtc ttgataaggt
    16261 cttcgggatt gaaactgacc atattgtaag tcatcaccgt tccgcccacc caggtagcta
    16321 cgaacgaata gaaaagagag ttgacatgac agcatggcat aacgagcagg tttgtgtcgt
    16381 caaaattaaa ctgatggtcg aaaatcataa tgaaatactg ggcaaaaagc gaaaggtgtg
    16441 tttttacaac gcctttcggc ttccctgttg ttccgccggt atacatgatg acccacgggt
    16501 catcggctgt tacaacagtt gccggctctt ccgggcttgc ttttgcaagc gcttcttcat
    16561 aggatacaaa accgtcaact ttggggttgt catggccaaa aaccatataa ccggttactg
    16621 tggggatgtt ctttttcatc tctgtgactg ttttgggcca tgggaactcc ttaccgtcag
    16681 ccttatcaac acccccctga acgataaaga ccttacattc gctgtgattg atgttgtatt
    16741 ccatttcagc cggtgcgagt ctgaacatca atggtacaca gataaacccg ccctttgctg
    16801 cagcagcgta tatttccatc cattcaacgc aattgtatgc gagtactgca aaccgatcac
    16861 cttttttcat tcccatgtct gcaagggcgt ttgcaagcct gcacgctctc tcatcccact
    16921 ccttaaaggt ataggatttg tacaagtctt taataccaat ctttttgggc catttgaagg
    16981 catttaccct cagcacgtct tttgctgtca tccagcgtcc atacctcata gttgctcctc
    17041 ctcaattata ttatcgttca tgatgcaaaa accaaccgat gctacattta ctcgacaagc
    17101 tccaggagat gatacacttt ctgcggcatc ttgcttctga gcgtgttatc gataaggtta
    17161 atcatgcaac cagggcaagc agtaacaaca atatctgctc cggttgcctt gatgccatcc
    17221 atctttttat cagcaatctt ctttgtaata tcgtaatgat atacgctaaa cgaaccaccc
    17281 atgccacagc aaaggtcagc attcggcatc tccacatact tcaagccttt aagtgctttc
    17341 agaattgccc ttggctgatc cttaatattc tgatacctga tgagatggca aggatcatgc
    17401 catgtagcag ttttaccctc aaactctttt ttcagtttga attcctcggg tggaatcttc
    17461 agaacatcaa taacaaattc tgtcatgtct ttgatttttt tctcgaaggc ctcgtatctc
    17521 ttcttctggt cctcattgtc aggcagatat ttctggtaat ccttaagggt ggaactgcat
    17581 gtcccacacg ccgtaacaat gtaatccaca tcctggaatg ccttgatatt ctgttcggca
    17641 agcattctgc ctgtctcaaa atcaccgcta aagtatatag cagcgccaca gcagctttgt
    17701 tcttcaggaa caattacttc aatccctctt ttcgtgagaa aatcgattac cttttcgcct
    17761 aattcaggat agataaagtc agttgcgcat cccatgaagt aaccgaccct cattttcggt
    17821 tccttgccgt tttgtggttt attgaccctt tttaccttat cacgcaggaa tacctttgca
    17881 atctccggta tgtttctccc ctgccctaat gccttgagga aatcaggcaa atgccgtacc
    17941 ttgccttctg ttttcggcat gagccactgg aactttgacg caagttttac atagcgaccg
    18001 aaagctttcc tattcgccat caccttcctg aaggcaaagt tcttgataaa tggtagccct
    18061 ttatttctta ccatctgggc tcgtgcaccc actacaaccc tgtcgatctg tgtttttgca
    18121 ggacacatgg ctacacatct cttgcagagc aggcattttc ctataatttc acccatttta
    18181 ggcgtgaatt cctgctcccc cttcaacaca agctttgtga gatagttctt acctcttgcc
    18241 actgctgttt cttccttttc ttcctggtac acaggacaga aaaaactgca aaaaccacac
    18301 ttcatacact ggtcagcata ttgttctatc tttttcagct cttttagatc ttccaagctc
    18361 aacccccttg ttggctattc ccagatttta cccgggttca tgatgttgtt aggatcaaat
    18421 agcgctttaa tctttttcat catatcaatt acagcaggtt caagctgcct tttaaggaac
    18481 ctctgttttt cgagacctat cccatgttcg cctgaaagca caccacctaa atccaccgca
    18541 ccttctataa tctcatccat ggccttaact gccctgtcat aatgttcttt attattaata
    18601 tccgtcaaga ttgcgggatg taagtttcca tctcccgcat gaccaagaac tacaatttca
    18661 acgttatatt tctttgccag ttccttgcac ttgttaataa gcgctggtat ttgccccctt
    18721 ggtactgtaa catcctctgc aaaaactgtt tttgctttcc caaaaacagc agcaaatcct
    18781 gctttccgtg ctgtccagta cttatcggcc tcagcctgat ccggtgcaac gcgcacatct
    18841 gttgccccat atttcttggc aatctctacg atcttttgtg tttctttttc aacagcttca
    18901 gggataccat ccacctcaaa aagaagaact gcgtcagcat ctttcgggag tcccatgggc
    18961 atcatttctt caattctgtt gataacccag ttgtcaagaa gctct
//
LOCUS       DAOOMC010000050        18689 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdus sp. isolate AMR_MDS_2685
            NODE_3034_length_18689_cov_6.172319, whole genome shotgun sequence.
ACCESSION   DAOOMC010000050 DAOOMC010000000
VERSION     DAOOMC010000050.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864760
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdus sp. (wastewater metagenome)
  ORGANISM  Syntrophorhabdus sp.
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae; Syntrophorhabdus.
REFERENCE   1  (bases 1 to 18689)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18689)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,729
            Genes (coding)                    :: 2,708
            CDSs (with protein)               :: 2,708
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 7 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18689
                     /organism="Syntrophorhabdus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3034_length_18689_cov_6.172319"
                     /isolate="AMR_MDS_2685"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2699756"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            50..574
                     /gene="cobO"
                     /locus_tag="PK125_06655"
     CDS             50..574
                     /gene="cobO"
                     /locus_tag="PK125_06655"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR00708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HPB37823.1"
                     /translation="MEKKGIIIVFTGNGKGKTTAALGIALRASGYGMKTLMIQFIKEK
                     GKSGEHNVCPSLNENIDIQPYGMGFVFRGDDLRPHIAMAEEAWFLMEDALRVGNHHIL
                     ILDELSVVMNLGLLTMERVTEFLAKKPADLHVIITGRDAPEEIIRLADVVTEMKEIKH
                     IYQQGVEAIKGIDY"
     gene            complement(656..1525)
                     /locus_tag="PK125_06660"
     CDS             complement(656..1525)
                     /locus_tag="PK125_06660"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HPB37824.1"
                     /translation="MEKERISDILLLCVAAIWGINFVAMKFLLVHISPVNLVLVRFFI
                     GSILLFFLLLFSGDVKVPLRDFIYLCFLGVIGITMYQFFFTYALKYASVTNVSIIINS
                     APLYGGILSSFFGFEKFNKKKILCILPGFFGVFIIVTKGTFSLESGNATGNILAVVSS
                     VLWALYTILSKPFLDRHSPLKVTTYSMITGSVLLIPFAPFCLDLNELMMLSAAGWAIM
                     SFTVVFSIVIAFYLWYRGVKKIGPSQTIIYQYSIPVFAAFFAYLLIHEQLYFSQLMGA
                     SIVFLNLWLARRS"
     gene            1579..2310
                     /gene="pyrF"
                     /locus_tag="PK125_06665"
     CDS             1579..2310
                     /gene="pyrF"
                     /locus_tag="PK125_06665"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF001273.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HPB37825.1"
                     /translation="MEPKEHFIFALDVEHFEEAQKLVLEFKDHVGMFKVGKQLFTHCG
                     PKIVDFIKLKNSKVFLDLKYHDIPNTVSRASTEAAKLGVDMLNVHTMGGFTMMNETRQ
                     AVQNAVKSLSIHRPILIGVTVLTSLDNAELERMGIHISVDTLTRNLALLAKDAGMDGV
                     VAGGSEIELIRELCGKDFVIVTPGVRIGEKKDDQKRTIDPVEAIRRGATYIVLGRAVR
                     DNPNPAALLDKIGRDIAYATSSEKK"
     gene            2285..3028
                     /locus_tag="PK125_06670"
     CDS             2285..3028
                     /locus_tag="PK125_06670"
                     /inference="COORDINATES: protein motif:HMM:NF012797.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HPB37826.1"
                     /translation="MQHLQKKNSIIGTMHEHRESVHRLWIEKGYEHVSNEIIQEAKRL
                     GIPFKIIPREAFMRKCKDPNSHVHLEQDEVACIDPGLFIKQITTQPDPVICAFDGIYD
                     PQNLGNIMRSAACLETNGIIIPKDRACGITRTVASVSRGGSERVPVVRVINLSRYMTD
                     LKKSGFFCYGLDERGTVPLWEANLTGPVCLIFGNESGLRRLTKETCDQILRIPASPDF
                     TSLNVATSFAIAVYEAKRQRALKAVTRNQ"
     gene            3185..4096
                     /locus_tag="PK125_06675"
     CDS             3185..4096
                     /locus_tag="PK125_06675"
                     /inference="COORDINATES: protein motif:HMM:NF040786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium metabolism-associated LysR family
                     transcriptional regulator"
                     /protein_id="HPB37827.1"
                     /translation="MLFTMDLRHLETFVKIAELNSFTKAAEELYITQPTVSKQITDLE
                     TYFGVKLIDRTKRRVALTRAGEVLLKYGKEFMVLRKETIDTMEAFKGLKKGVIIVGAS
                     TIPGIYILPHILNTFRQQYSGIQVKLVVSDSKDIMDKMEQGQIDVGFVGARDNSKKLE
                     FRKFFEDTIVIIAPSGYPPSIALENIAEYPFIMREQGSGTRNFFHSALKKAGINPVSD
                     LQVVAELTDTEAIKEAVKSGMGISCVSKMAVNGALSQGHMIVMEVTGMEDLKRSFFLV
                     NRKGKSLLPQVKALIEIIDRWKEYDKV"
     gene            4083..5363
                     /locus_tag="PK125_06680"
     CDS             4083..5363
                     /locus_tag="PK125_06680"
                     /inference="COORDINATES: protein motif:HMM:NF015980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB37828.1"
                     /translation="MTKFKCVIVTLLLVFLAGTAESKVYLDVYGKTYKKITIGVPSFQ
                     SDRPDRLRVDMSELINQDLDMSGFFIVAPNSLLDRELTEEGIEKKDIKFTNWRSLGIE
                     LLCKAKFIMSNEEIALESYLYDTLDGSLLLAKRYKSRPEDWRRLVHRLADDILLAVTG
                     EKGIMSSRVVFVSGTKRNKDIYIGGIDGQNIRQVTNFHNITVSPSVSPDGRFLAYTSY
                     KEGKPNLFIMDLETGKDIHSEREEGMKLGTTWFGKSTLGYAKTSGKQSSIYTFDAAGR
                     QKKTLFTKEGILASPSFSADGTKMAFVSDMYGSPQVFIRDNSSGEVKRLTYSGNYNAS
                     PSFSPKGDLIAFVARINGSFEICTMNTDGSNQRVLTSGGINDSPHFSPCGRYIIYSSK
                     KGDQYNAYIMLYNGENKRMLKLVNKDTEQPKFVP"
     gene            5526..6101
                     /gene="pal"
                     /locus_tag="PK125_06685"
     CDS             5526..6101
                     /gene="pal"
                     /locus_tag="PK125_06685"
                     /inference="COORDINATES: protein motif:HMM:TIGR02802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HPB37829.1"
                     /translation="MKYLAILLAVVMITCYGCASKPVQTGQKPLTAEEQARLDQEEAL
                     KRKEREARISEEELARSRAERERLERERALETQKSIPVSDVYFEYDSYTVQPEDIAKI
                     KDMGEWLKANSGLRIVVEGHCDERGTVEYNLALGQKRAEVVKNHLVKFGVDEKRIRTI
                     SFGKEMPADPGHNEDAWAKNRRVHFATDQKG"
     gene            6107..6883
                     /gene="ybgF"
                     /locus_tag="PK125_06690"
     CDS             6107..6883
                     /gene="ybgF"
                     /locus_tag="PK125_06690"
                     /inference="COORDINATES: protein motif:HMM:TIGR02795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system protein YbgF"
                     /protein_id="HPB37830.1"
                     /translation="MHRRVFVTRFQVLFISLIVTFFLTNCATSEETTRLARNISSTDT
                     ELSRLRYENDRRFSELAKEDENIRKQLVNLSSSLDDKNEKIKVIFGKLDELEYQLRTY
                     FNELKNEINSLKKSGTASPGGPTITQRQTPQTPNRDYETAYKDAFEAFQKKSYEEAIK
                     KFTAFIEVHPGTPLAPNALYWIGESYMNIKNYEKAILSFQELVDKYPKSDKVPRALLA
                     QADAFNAVKDKKSSTTVLKKVIELFPKSEEAAIAERRLRN"
     gene            complement(6880..11070)
                     /gene="rpoC"
                     /locus_tag="PK125_06695"
     CDS             complement(6880..11070)
                     /gene="rpoC"
                     /locus_tag="PK125_06695"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR02386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HPB37831.1"
                     /translation="MEDYFKVYDKQKDPLSYTAIKISLASPELIEKWSYGEVKKPETI
                     NYRTFKPERDGLFCAKIFGPVKDYECICGKYKRMKHRGIICEKCGVEVIQSKVRRERM
                     GHIKLACPVAHIWFLKSMPSKIGTLLELTIKEVERVLYFEMYIVIEPASSPYDFCELI
                     SEEKYLEAMEKYGPDFVAGIGAEAMRECLKKIDVDTLTKQLRDEMRETSSDAKKKKLA
                     RRLRVVDAFKVSGVKPEWMILDIIPVLPPELRPLVPLDGGRFATSDLNDLYRRVINRN
                     NRLKRLMELNAPEIIIRNEKRMLQEAVDALFDNSKRGRVVTGTSKRPLKSLSDMLRGK
                     QGRFRQNLLGKRVDYSGRSVIVVGPELRLHQCGLPKYMALELFKPFVYNRLIKKGFAT
                     TIKNAKKIVEKERPEVWDVLDEVIKEHPVLLNRAPTLHRLGIQAFEPHLIDGKAIQLH
                     PLVCPAYNADFDGDQMAVHIPLSVEAQTEARVLMMSTNNILSPANGRPIIVPTQDIVL
                     GLYYLTIERKCRKGEGKVFADPEEVRIAYDSGEVDLHSRVKVRLGGTLVETTAGRIML
                     RDIVEDALSELEPDRRRSVLDEMFSIINRVMDKKTIGLLVDKCYREAGEKCTVILSDK
                     LKDLGFHYATLGGISICIDDMKVPDKKKQLIDRANKDVLDVQRQHAEGLITDGERYNK
                     VIDIWANVTEEIAKELMDELGSEKVVDLEGKPVISPSGKPEYQNSLNPIFIMAQSGAR
                     GNAQQIRQLAGMRGLMAKPSGEIIETPITSNFREGLDVLQYFVSTHGARKGLADTALK
                     TANSGYLTRRLVDVSQDVVVSEHDCGTFDGIEVGALTEGGEIIERLDARILGRVVLED
                     LKDPDTGEIIVRRNEEITESHLKKIEDAGFEKIRIRSVLTCRSRRGVCVLCYGRDLSR
                     GRLVSIGEAVGIIAAQSIGEPGTQLTMRTFHIGGAASRRVEQSTLEARNDGYVKLLNV
                     KTILNREGVPVVMNRNGEVAIEDETGREREKYHVIYGAKLKIEEGQKVKSGHVLAEWD
                     PYTIPILSEVSGKVKFGDIIEGETMQESKDELTGLSYKVVMEPKDPEKRPRVSIKDDK
                     GKTIIVPDATSPARYILPVGAHIVVNEGDDIHAGDVVSKMPRETTKTKDITGGLPRVA
                     ELFEARRPKENAIVSEVNGFVSFGKMSKGKREIVVTPEYGDPKKYSIPRGKHIIVHEG
                     DYVKAGEALMDGPVNPHDVLHILGIKDLARYLVDEIQEVYQLQGVKINDKHIEVIVRQ
                     MLKRVRIKDVGDTNFIMDEAVEWWIFEEENRRVMEAGGRRAEAEPLFLGVTKASLITD
                     SFISAASFQDTTKVLTQAAIEGRVDYLRGLKENVIMGRIIPAGTGFLKYRIYDMVTKD
                     KITPEAIEQQPAETEQAV"
     gene            complement(11100..15215)
                     /gene="rpoB"
                     /locus_tag="PK125_06700"
     CDS             complement(11100..15215)
                     /gene="rpoB"
                     /locus_tag="PK125_06700"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: protein motif:HMM:NF001616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HPB37832.1"
                     /translation="MRETFNNKIFRKNYGKIKEILDIPNLIEIQLDSYINFLQRDIAP
                     EKRENIGLQAVFKSVFPIKDSHDTTSLEFVKYEMGDPKNSEEECLFRGLTFAAPMKVT
                     IRLVVWNIDSITKTKDIRAVKEQDVYFGEIPLMTERGTFIINGTERVIVSQLHRSPGV
                     YFDADQAKSTTSGTLAYTARIIPYRGSWLDFEFDSKELVYVRIDKKKKIPVTLLLKAL
                     GYTEKEILDYFYQKERVIIKEGKLYKETPLALLIGQKAHHDILNPKTDEVLIKKHKKI
                     SRADVKKMEAANIKEIPVEEDDIIGRVTSSEIIDPKTKEALIPINKLITKEDLETIAK
                     KKVKTFEVLFIDNLNVSPSLRNTLETDKVNTKEDALLEIYRKLRPGDPPTIEFAEALI
                     HNMFFSPERYDLSRVGRLKINHRLSLDIPLDVSTLRRDDIIEVVKHLLELKDGRGAGD
                     DIDHLGNRRVRTVGELLENQFRMGLVRMEKAIKERLTFPEMETLMPHDLVNPKPVATA
                     VKDFFASSQLSQFMDQTNPLSEITHKRRLSALGPGGLTRERAGFEVRDVHPTHYGRIC
                     PIETPEGPNIGLIASLSTYAKVNEYGFIESPYRKVEKGMVTDEVQYLSALEEEQFFIA
                     QANSPVDEHGKFKEPLVSAQKGGGFYLIGPDQVDYMDVSPKQLVSASASLIPFLEHDD
                     ANRALMGSNMQRQAVPLIITEAPIIGTGMEKVVGRDSRVTITAKHSGVIKSVDANRII
                     LKFEEKSKHDEPVTKIDVHNLLKFRRSNQDTCINQRVIVKEGDFVKKGDVLADGPSTD
                     RGELALGKNVLVAFMPWRGYNYEDSVLINERLVKEDVFTSIHIEELECVVRDTKLGKE
                     EVTRDIPNVGDETLKDLDESGIIRIGATVKPNDILVGKVTPKGETQLTPEEKLLRAIF
                     GEKAGDVKDTSLRVPHGIEGIVTDVKVLSRRGIDKDDRSRDIEDKEVSNVLKDQEDQV
                     KIILESTREKLNSLFLGKTATADIKDGRGRTAIKKGDIFTKEKIDHISIDKLQDVDFN
                     DNELEAKISKILDSKENQIEIIKLYYEDKINKIKKGDELPPGVIKTIKVYVAMKRKVA
                     VGDKISGRHGNKGIVSVILPEEDMPFMEDGTPIDIVLNPLGVPSRMNAGQILETHLGW
                     AAKELGKRIGDLVDGYYRASCASDAIKAYLKKIFNRGELKSFIDKLSEEEVVALANTM
                     REGIRIAVPVFDGAKENEIKDLFKMAGLADSRQTILYDGRTGEPFHHKITVGYMYFLK
                     LHHLVDDKIHARSIGPYSLVTQQPLGGKAQFGGQRLGEMEVWALEGYGAAYSLQEFLT
                     IKSDDVNGRIRAYEAIVKGEDVLEPSLPESFNVLVKELQSLCINVDLVKEE"
     gene            complement(15323..15718)
                     /gene="rplL"
                     /locus_tag="PK125_06705"
     CDS             complement(15323..15718)
                     /gene="rplL"
                     /locus_tag="PK125_06705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HPB37833.1"
                     /translation="MSVTKEDVIQFIENMTVLELSDFIKDLEEKFGVTAAMPVMAAGA
                     APAAAGAAPVVEAAEEKTEFDVILTGYEADKKIQVIKVVRAITSLGLKEAKDLVEGVP
                     KPVKEGVSKDEAANIKKQLEEVSGQVKVE"
     gene            complement(15731..16252)
                     /gene="rplJ"
                     /locus_tag="PK125_06710"
     CDS             complement(15731..16252)
                     /gene="rplJ"
                     /locus_tag="PK125_06710"
                     /inference="COORDINATES: protein motif:HMM:NF000955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HPB37834.1"
                     /translation="MERQKKEKIVEELKAKLASLNAMFLAEYSGTNMSQMTKLRRELS
                     NVDVEFNVVKNTLLKIASGGTKAEVLQDKFKGPNAIICINKDPSSAAKVLSGLAKDIP
                     NLKLKGGFLGTRVITSEEILKLATIPSKDVLVGKFLGLLQGMPQRFLYVLSGNMNKLM
                     MTLNAIKIQKEQG"
     gene            complement(16379..17083)
                     /gene="rplA"
                     /locus_tag="PK125_06715"
     CDS             complement(16379..17083)
                     /gene="rplA"
                     /locus_tag="PK125_06715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008810408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HPB37835.1"
                     /translation="MAKIAKKYAEAIKKVDREKRYDMEEALDLLPQMTYAKFDETVEI
                     ALRLGVDPRHSDQMVRGSVALPNGLGKKVRVLVFAKGQKEKEAEEAGADFFGAEELIE
                     KIQKGWLDFDKAIATPDMMAMVSKLGKVLGPRGLMPNPKVGTVTFDVAKTVKEMKAGR
                     VEFRVDKAGNLHIPVGKISFGKEKILENVNSLLDAIVRLKPPSSKGTYVKGIAICTTM
                     SPGIKIDPAYVRNLSR"
     gene            complement(17197..17625)
                     /gene="rplK"
                     /locus_tag="PK125_06720"
     CDS             complement(17197..17625)
                     /gene="rplK"
                     /locus_tag="PK125_06720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013028894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HPB37836.1"
                     /translation="MAKKVVALVKLQLQAGQATPSPPVGPALGQHGVNIMEFCKAYNE
                     KTKTQEGTVIPVLITIFSDRTFTFVTKTPPAAFLIKRAAKLAKGSNNPKKEMIGSITK
                     SQVKEIAQLKMVDLNAKNIEGAMKIIEGSVRSMGLEVVEG"
     gene            complement(17638..18171)
                     /gene="nusG"
                     /locus_tag="PK125_06725"
     CDS             complement(17638..18171)
                     /gene="nusG"
                     /locus_tag="PK125_06725"
                     /inference="COORDINATES: protein motif:HMM:TIGR00922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HPB37837.1"
                     /translation="MEKKWYVVHTYSGYEQKVKENLEENIKNVKMEEFFGDIIVPSEI
                     IMEKVKGQVKKSQRTVFPGYIIVNMVMNNDTWYLVRNTPKVTGFVGYDKMNPPPLPDH
                     EVDEIIYAIQEGKGKIKPKIRFEKGDGVKVTEGPFANFTGNVEEIKPEKGKVKVLLNI
                     FGRTTPVELDFIQIEKI"
     gene            complement(18197..18400)
                     /gene="secE"
                     /locus_tag="PK125_06730"
     CDS             complement(18197..18400)
                     /gene="secE"
                     /locus_tag="PK125_06730"
                     /inference="COORDINATES: protein motif:HMM:TIGR00964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HPB37838.1"
                     /translation="MDVKEKIEVVKAYFREVYLEGKRITWPSRKDALKGTYIVLITVF
                     ISAIFLGIVDVGLARLIQAILRG"
     gene            complement(18418..18491)
                     /locus_tag="PK125_06735"
     tRNA            complement(18418..18491)
                     /locus_tag="PK125_06735"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(18455..18457),aa:Trp,seq:cca)
     gene            complement(18493..18642)
                     /gene="rpmG"
                     /locus_tag="PK125_06740"
     CDS             complement(18493..18642)
                     /gene="rpmG"
                     /locus_tag="PK125_06740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001265708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HPB37839.1"
                     /translation="MRQLVTLACTECKNRNYTTTKNKQKTPGRLEIKKFCRFCKRHTP
                     HRETK"
BASE COUNT         4865 a         4441 c         3922 g         5461 t
ORIGIN      
        1 ccatcacgcg tcattgatca cggatcacgg tatttaaggg ggggtgtgca tggagaaaaa
       61 aggaatcatc attgtattta ccgggaacgg caaaggcaag accacagcgg ctcttggcat
      121 tgcgcttcgt gccagcggtt atggcatgaa aaccctcatg atacagttca taaaggagaa
      181 ggggaaatct ggcgagcaca atgtgtgtcc ttccctcaat gaaaatatcg atattcaacc
      241 ctatggcatg ggattcgtct tcagagggga tgatctcagg ccccatatcg ccatggccga
      301 agaagcctgg ttcctcatgg aggatgccct ccgggtgggg aaccaccata tcttgattct
      361 cgatgaactc tctgttgtga tgaacctcgg gcttctcacc atggaaaggg ttacagagtt
      421 cctcgcgaaa aaaccagcgg atcttcatgt tattataaca ggccgtgacg cgcccgaaga
      481 gataatcagg cttgctgatg tggtaacaga aatgaaggaa ataaagcata tctatcagca
      541 gggggtagag gccatcaagg ggattgacta ttaagaaatg gtttatcgtt gtctcgcaac
      601 tggctgtttt gccgatcata tgccgtacat tacgcgtcac caccccctca ttcggtcagc
      661 ttcttcgtgc cagccagagg tttaagaaaa cgatggatgc ccccatcagt tgggaaaaat
      721 aaagctgctc gtggatgaga aggtacgcaa aaaatgcagc aaatactgga atagaatatt
      781 gataaattat ggtttgtgag ggtccgatct tttttacccc tctgtaccag agatagaatg
      841 cgatgacaat agaaaataca acggtaaagg acattattgc ccatcctgct gcggagagca
      901 tcataagctc attcagatca agacagaatg gagcgaatgg aatgaggaga acagatcctg
      961 ttatcatact atacgttgtt actttaaggg gtgagtgacg gtcaagaaat ggcttggaca
     1021 ggattgtata gagagcccag aggacgctgg acaccacagc aaggatgttt cccgtagcat
     1081 tgcctgactc gagggagaag gttccctttg taactatgat aaaaacccca aaaaatccgg
     1141 gaaggataca gagaatcttt tttttgttaa acttttcaaa accgaagaag ctgctgagaa
     1201 tccccccgta gaggggtgct gaattgataa taatgctcac attcgtaact gaggcgtatt
     1261 tcagtgcata ggtgaagaag aactggtaca tggtgatacc aataacgcca agaaaacaca
     1321 ggtaaataaa atccctgaga ggcaccttta catctcctga aaaaaggagt aaaaaaaata
     1381 agagaatact cccgatgaaa aacctgacga gcacaaggtt taccggggag atgtggacga
     1441 gaagaaattt catggctaca aaatttatcc cccagatggc agctacacaa agaagcagaa
     1501 tgtcagatat cctttctttt tccacagcag aagattaatg aatggtctat agtttgtcaa
     1561 atcaatacag gaggtagtat ggagcctaaa gaacatttta tttttgcatt ggacgtggag
     1621 cattttgaag aagctcagaa acttgttctg gagttcaagg accatgtagg aatgttcaag
     1681 gtaggtaagc agctttttac ccattgtggc ccaaagatag ttgatttcat taaattgaaa
     1741 aattctaagg tttttctcga tctcaagtat catgatatac cgaacactgt gtccagggca
     1801 tccacagaag ctgcaaagct cggagtggac atgctgaacg tgcatacaat gggcggtttt
     1861 actatgatga acgaaacaag acaggccgtc cagaatgcgg tgaaatccct ttccatacat
     1921 cgtcccatac taatcggcgt aacagtcctt acaagcctgg acaatgctga gctggaacga
     1981 atgggcatac atatctccgt agacaccctc accagaaacc tggcacttct cgcaaaagac
     2041 gcaggcatgg atggagtggt cgcagggggc agcgaaattg agctgatcag ggagttatgc
     2101 ggaaaggatt ttgtcattgt aacgccgggg gtaaggattg gggaaaaaaa ggacgatcag
     2161 aaaagaacca ttgaccctgt ggaggcaatc aggcgcggcg ccacatatat tgttcttgga
     2221 agggcagtga gagacaatcc taatccggcc gcccttttgg ataaaattgg cagggatata
     2281 gcctatgcaa catcttcaga aaaaaaatag tatcatcggt acaatgcatg aacaccggga
     2341 aagtgttcac aggctctgga tagaaaaggg gtatgagcat gtctccaacg aaattattca
     2401 ggaagcgaaa aggctgggga taccattcaa aattatccct cgcgaagctt ttatgaggaa
     2461 atgcaaagac ccaaattcac atgtacattt agagcaggat gaggttgcct gtattgatcc
     2521 cgggctgttt atcaaacaaa ttacaactca acctgacccg gttatctgtg cttttgacgg
     2581 tatctatgat cctcaaaatc tcgggaatat catgagaagc gccgcatgcc ttgaaacgaa
     2641 tgggatcata atcccaaaag acagggcatg tggcatcacc agaacagtgg catccgtgtc
     2701 acggggaggt tcagaacgtg ttcctgtggt gcgggtaata aacctgtctc gatacatgac
     2761 cgacctgaaa aaatcaggat ttttctgcta tggacttgac gaaagaggaa cagttcctct
     2821 ctgggaggca aatctcacag gccctgtttg cctgatattt ggaaatgaaa gtggtctgag
     2881 aaggctaaca aaggaaacct gtgaccagat cctcaggata cctgcatcac ctgattttac
     2941 ctcccttaac gtggcaacaa gctttgccat tgcagtgtac gaagcaaaaa ggcagagagc
     3001 attgaaagcc gtaacccgta atcagtaatg catgatgtgt ttgataaaca gcaatcactt
     3061 acggattaca cgtcacagca tttcttttta ctttccattt ctttcttctt ttcccgccat
     3121 tcacgctatc cccgcttatt tttgttacgg gttatttttc ttgaatggaa ggtatagata
     3181 tactatgctt ttcactatgg atttacgcca tctggaaacg tttgtgaaga ttgctgagct
     3241 caatagcttt accaaggcag cggaggagct ttacattact cagcccacag tgagcaaaca
     3301 gattacagac cttgagacct attttggtgt gaagctgata gacaggacta aaagacgggt
     3361 agccctcacg cgggcaggtg aagttctcct caaatacggg aaggaattca tggtgctgag
     3421 aaaagagaca atcgatacaa tggaggcatt caagggctta aaaaaagggg tgattattgt
     3481 aggggcaagc accataccag gcatctatat ccttcctcac atactcaaca cctttcgaca
     3541 acagtacagc ggtattcagg tcaagctggt agtatctgac tccaaagata tcatggacaa
     3601 aatggagcag ggccagattg acgtaggatt cgtaggcgcc agagataatt caaaaaaatt
     3661 ggaattcaga aaattcttcg aagacacaat cgtcatcatt gctccctctg gttaccctcc
     3721 atccattgca ctggaaaata tagcagaata cccctttatc atgcgcgaac aaggttcagg
     3781 aacaaggaat ttttttcatt ctgcattaaa aaaagcaggg atcaacccgg tatcagactt
     3841 acaggttgta gcggaactca ctgatacgga ggctattaaa gaggcagtaa agagcggtat
     3901 gggcatctcc tgtgtttcaa aaatggcagt aaatggggct ctatctcaag gacacatgat
     3961 cgttatggag gtaacaggaa tggaggatct caagcgatca ttttttcttg tcaatagaaa
     4021 agggaaaagc cttctcccgc aagtaaaggc cttgatagaa attattgaca ggtggaaaga
     4081 atatgacaaa gtttaaatgc gtgatcgtta cactacttct cgttttcctt gcagggactg
     4141 ctgagtccaa agtgtatctt gatgtgtatg gaaaaacata taaaaagatc accataggcg
     4201 tgccatcttt tcaaagtgac aggccagaca ggctccgggt ggacatgagc gaactcatta
     4261 accaggacct tgacatgtcc ggctttttca ttgtggctcc caactccctc cttgaccggg
     4321 aacttactga agagggaata gaaaaaaagg atataaaatt caccaattgg cgttccctgg
     4381 gaattgagct tttatgtaaa gcaaagttca taatgtcgaa cgaagagatt gccctcgaat
     4441 cttacctgta tgataccctt gacggttctc ttctccttgc taagcgttat aaatcgagac
     4501 cggaagattg gagaaggttg gtgcatcgcc ttgccgatga tattctcctt gcagttaccg
     4561 gtgaaaaagg cattatgagc tcgcgggtcg tttttgtctc tggcacaaaa aggaacaagg
     4621 acatatacat tggcggcatt gatggacaaa acatcaggca agtgacgaat tttcataata
     4681 ttaccgtgtc accatcagtc tccccggatg gaagatttct ggcatacacc tcatataagg
     4741 aaggtaaacc aaaccttttc ataatggatc tggaaacagg taaggacatt cattctgagc
     4801 gggaagaagg tatgaagctc ggcacaacgt ggtttggaaa atcgaccctt ggttatgcga
     4861 aaacctctgg caaacaatca agtatataca cctttgatgc tgcgggccgt caaaaaaaga
     4921 ccctgtttac aaaagaagga atactcgctt caccctcttt ttccgctgat ggaacaaaga
     4981 tggcctttgt ttcggacatg tatggcagcc cacaggtgtt cataagggat aattcatcag
     5041 gtgaagtgaa aagactcacc tattcaggga attacaacgc atcgccatcc ttttccccaa
     5101 aaggagatct catagccttt gtagcgagga ttaacggctc tttcgaaata tgcactatga
     5161 atacggacgg ttccaatcag agagtcctca ccagcggagg catcaatgat tcaccacatt
     5221 tttcaccctg cggcagatat attatttatt cgtcaaagaa aggtgatcaa tacaatgcct
     5281 atattatgct gtacaatggc gagaataaaa gaatgctaaa gctcgtcaat aaggatactg
     5341 aacaaccaaa atttgtacca taagcgacag tgttacgttc cgggttatta gttttaagtt
     5401 gatgaatctt acggaagata cggcaccacc ccattttggg cgggaaagga aggctccgac
     5461 agcgtagctg gtgtaagtgg agagatgcga accccagtta attgaaactg aagcaggagg
     5521 ttaaaatgaa gtatttagca attttattag ctgttgtaat gatcacatgt tatggatgtg
     5581 catccaaacc agttcaaact ggacagaaac ccctgaccgc agaagaacag gccaggcttg
     5641 atcaggaaga agcactgaaa agaaaagagc gggaagcaag gatcagcgag gaagagttgg
     5701 cacgatcacg ggcagaaagg gaacgccttg agagagaaag agctctcgag acacagaaat
     5761 ctatccctgt aagcgatgtt tattttgaat atgacagtta cactgttcaa cccgaggata
     5821 tcgcaaaaat aaaggatatg ggcgaatggc ttaaagcgaa tagtggatta cgtatcgttg
     5881 ttgagggtca ctgcgatgaa aggggcaccg ttgaatacaa ccttgccctt ggtcaaaaaa
     5941 gagctgaagt tgtaaaaaat catcttgtga agtttggcgt agacgagaaa agaattcgaa
     6001 caatctcctt tggcaaagaa atgccggctg atcctggcca caacgaagat gcctgggcaa
     6061 aaaaccgtcg cgttcatttt gccacagacc agaaggggta aaaaatatgc atagaagagt
     6121 ttttgtaaca cgttttcagg tccttttcat atcacttatt gtgacatttt ttctcacaaa
     6181 ttgcgccacc agcgaagaga cgacgcgatt ggcacggaat atttcttcca cagacacaga
     6241 actgagcagg ctgagatatg aaaacgaccg acggttctca gagcttgcga aagaggacga
     6301 aaatatcagg aagcagcttg tcaatctctc ttcatccctt gatgataaaa atgaaaagat
     6361 caaggttatt ttcgggaaat tggatgaatt ggaatatcag ctacggacct acttcaatga
     6421 attgaaaaat gagattaatt ccctcaagaa atccggcact gcttctcctg gaggtcctac
     6481 catcactcag cgccagacgc cccagacccc taaccgggac tacgaaacag catacaagga
     6541 tgccttcgag gcttttcaaa agaaatctta tgaagaagca atcaagaagt ttactgcttt
     6601 tatcgaggtg catccaggaa caccgctcgc accaaacgcc ctgtattgga ttggcgaaag
     6661 ctatatgaat atcaaaaatt acgaaaaagc cattctttcc ttccaggaac ttgttgacaa
     6721 gtatccgaaa agcgataagg tgcctagggc cctgcttgca caggccgatg cctttaatgc
     6781 tgtgaaagac aagaagagct ctacgactgt gctcaagaaa gtcatagagc tcttccctaa
     6841 aagcgaagaa gctgctattg cagaacgaag actgaggaat tagacagcct gttcggtttc
     6901 tgctggttgt tgttcgatcg cctccggcgt tattttgtct tttgtaacca tatcatagat
     6961 tctgtatttc agaaaacctg tgcctgctgg aataatgcga cccatgataa cattttcctt
     7021 gagacctctc aggtaatcga cacgaccttc aatcgctgcc tgagtcaaaa ctttcgttgt
     7081 atcctggaaa cttgctgcag aaataaaact atccgttatc agcgaggcct ttgttacacc
     7141 aaggaacaat ggttccgctt cagcacgtct gccgcctgcc tccattaccc tcctgttctc
     7201 ttcttcaaaa atccaccact caactgcctc atccatgata aagttcgtat cgcctacatc
     7261 ttttatcctg acacgcttca gcatctgtct gacgataacc tcgatatgtt tgtcgttaat
     7321 cttaacaccc tggagttggt agacctcttg aatctcatcc acaagatatc tcgcaagatc
     7381 cttgattccc aaaatatgga gaacgtcatg agggttcaca ggtccatcca taagcgcctc
     7441 accagccttc acgtagtcgc cttcatggac aatgatgtgc ttgcccctgg gaatactgta
     7501 tttctttgga tctccatatt caggggtaac aacaatctcc cttttacctt ttgacatttt
     7561 gccaaaggat acaaaaccat tgacttcgct gactatggca ttctcttttg gccgtcttgc
     7621 ctcaaaaagc tctgccaccc tgggaagacc tccggtaatg tctttggtct tggttgtttc
     7681 ccttggcatt tttgaaacaa catctcctgc atgaatgtca tccccttcgt tcacaactat
     7741 atgggcgcca acagggagta tatagcgggc aggactcgtt gcatcaggca caatgatggt
     7801 tttgccttta tcatccttga tggataccct cggtcttttt tccggatcct tcggttccat
     7861 gacaacttta tatgacaggc ctgtcagttc atccttgctt tcctgcattg tttcgccttc
     7921 gatgatatca ccgaacttca cttttccgga cacctcagag agaatgggaa tcgtataagg
     7981 atcccactct gcaaggacat gaccgctctt caccttctgt ccttcttcga ttttcagttt
     8041 tgcgccgtat ataacgtgat atttttctct ttctctgcct gtctcatctt cgatggcaac
     8101 ctcaccgttc ctgttcatga ctacaggtac accttctctg ttcaggatgg tcttcacgtt
     8161 aagaagtttc acataaccgt catttcgggc ttctagggtt gactgttcta cgcgccgcga
     8221 tgcagcaccg ccgatatgga aggttctcat agtaagctgg gtacccggct caccgattga
     8281 ctgggcagca ataataccaa cagcttctcc tatactaaca agcctacctc gtgagaggtc
     8341 ccttccgtaa caaagaacac atacccctct tcttgatcta catgtgagaa ccgaccggat
     8401 cctgatcttt tcaaagcctg catcttctat tttcttaagg tggctctctg tgatttcttc
     8461 attccgtctg acaataatct cacctgtgtc agggtctttc aggtcttcga ggacaaccct
     8521 tcccagtatt ctcgcatcaa gacgttcaat aatttcacca ccttcagtga gtgcacccac
     8581 ttcgatgccg tcaaaggtgc cgcaatcatg ttcagatacc acaacgtcct gcgatacatc
     8641 aacaagtctc cttgtcagat atcccgagtt tgcagttttt aatgcggtgt ctgcaaggcc
     8701 ttttcgtgca ccgtgggtag acacaaagta ctgaagtacg tccaacccct ctctaaagtt
     8761 gcttgtaatt ggtgtttcta taatctcacc agaaggcttg gccataagac ccctcattcc
     8821 tgcaagctgc ctgatctgct gggcattacc tctcgcaccg gattgagcca taataaagat
     8881 ggggttgagg ctgttctgat actctggttt cccactcggg cttatgactg gtttgccctc
     8941 aaggtcaacc actttttcag aaccgagttc atccattaac tccttggcga tctcctctgt
     9001 tacatttgcc cagatgtcga tgaccttgtt gtaacgttca ccgtcagtga tcaggccttc
     9061 tgcatgttgt ctctgaacat cgaggacatc tttgttcgct ctgtcaataa gttgtttctt
     9121 cttatctggt actttcatgt catcgatgca tatagaaata ccgccaaggg tagcatagtg
     9181 gaaacccagg tctttgagtt tgtcagagag aataaccgtg cacttctctc ctgcttcccg
     9241 gtaacacttg tccacaagaa ggccgatcgt ctttttatcc ataaccctgt taatgatgct
     9301 gaacatttcg tcgagaacag accgccttct gtccggttcc agctcagaca aagcatcttc
     9361 gactatatca cggagcatta tccttcctgc ggttgtttcc acgagcgtgc caccgagtct
     9421 gaccttaacc cgggaatgga gatctacctc tcccgaatca taggcaatcc tcacttcctc
     9481 agggtctgca aacactttgc cttcaccctt tctgcatttt ctctctatag ttaggtagta
     9541 taggccaaga acgatgtctt gcgtgggtac aatgatgggc ctaccgtttg caggcgacag
     9601 aatgttgttt gtggacatca tcagcacccg tgcctcggtc tgtgcctcaa ccgagagcgg
     9661 tatatgaacc gccatctggt ctccatcgaa gtctgcgtta tacgccgggc aaacaagagg
     9721 atggagctgg atggccttcc cgtcaatgag atggggctca aaggcctgga tgccaaggcg
     9781 gtgcagtgtg ggagccctgt tcaagagaac agggtgttcc ttaataacct cgtcaagaac
     9841 gtcccatacc tctggccgct ctttttccac aatctttttt gcatttttaa tggtagttgc
     9901 aaagcctttt ttgatgagcc tgttatagac aaagggcttg aaaagctcca gggccatata
     9961 tttaggaagc ccgcactggt ggagtctcag ttccggcccc actacgatga cagatctccc
    10021 ggaatagtcg acccttttcc caagaagatt ctgtctgaac ctgccctgtt taccccgaag
    10081 catatcactc agcgatttga ggggtctttt actggtaccg gtaactaccc gtccccgctt
    10141 agaattgtca aaaagagcat ccactgcctc ctggagcatt cttttctcat tccttataat
    10201 gatctcaggt gcattaagct ccataagcct ttttagtctg ttattcctgt taataaccct
    10261 cctgtagagg tcattgagat cagatgtggc aaaccttcca ccatccaggg ggacaagggg
    10321 acggagctcg ggtggaagaa ctgggattat atcgagaatc atccattccg gttttacacc
    10381 tgaaacttta aatgcatcca ccacgcgcaa gcgccgggcg agctttttct tcttcgcatc
    10441 gcttgatgtt tccctcattt cgtctctgag ctgtttcgtc agcgtgtcaa catctatttt
    10501 tttcaggcac tccctcatcg cctcggcacc gatgccggcg acaaaatcag gcccgtattt
    10561 ctccattgct tcaagatact tttcctcgga tatgagttcg caaaagtcgt acggtgatga
    10621 tgccggctca ataacaatat acatttcaaa gtagagcacc cgttcaactt ctttaatggt
    10681 aagttcaagc aaggttccaa tcttgctggg catgcttttc agaaaccaga tatgggcaac
    10741 aggacaggca agcttaatat ggcccatccg ctctctcctc acctttgact ggattacttc
    10801 aacaccgcac ttctcgcaaa tgatgccccg gtgcttcatc cttttatatt tgccgcatat
    10861 acattcgtag tccttcacag gaccaaagat cttggcgcag aaaaggccat ccctttcagg
    10921 tttaaaggtt ctataattga tagtttccgg tttcttcact tccccatatg accacttctc
    10981 tatcagttcg ggggaagcaa gtgatatttt gatggccgtg taactcaacg gatccttctg
    11041 tttgtcgtaa accttaaaat aatcttccaa tttactgcct ccttcgttaa aaggttacat
    11101 tattcttctt tcaccagatc cacatttatg cataagctct gcaactcttt taccaaaaca
    11161 ttgaatgact cagggagact tggctcaaga acatcctctc cctttacgat cgcttcataa
    11221 gcccttatcc ttccgttcac atcatccgat ttaatagtga gaaactcctg gagtgagtac
    11281 gcagcaccat acccctcaag cgcccatact tccatttccc caagtctctg accgccaaac
    11341 tgtgccttgc cccccagagg ctgctgggtg acaagagaat agggccctat ggaccttgcg
    11401 tggattttat catccacaag gtggtgcagc ttgaggaaat acatataacc gactgttatc
    11461 ttgtggtgaa atggctctcc tgtccttccg tcgtaaagga ttgtctgcct gctatcagcc
    11521 agacctgcca tcttgaaaag atccttgatc tcgttttctt tggcgccatc aaagacagga
    11581 acagctatgc gaatcccttc tctcatagta tttgcaaggg caacaacctc ttcttcgctc
    11641 aatttatcga taaagctttt cagttcgcca cggttaaata tctttttcaa ataggcttta
    11701 attgcatcag aagcacagct tgctctataa tagccgtcta ccaggtcgcc gattcgcttt
    11761 ccaagctctt ttgctgccca gccaagatgt gtttccagaa tctggccggc attcattctc
    11821 gatggaactc caagaggatt cagcacaata tcgataggtg taccatcttc catgaagggc
    11881 atatcttctt ctgggagtat taccgacaca attcctttgt tcccgtggcg gcccgatatc
    11941 ttgtcgccta ccgcaacctt tcttttcatg gcaacataga ccttgattgt ctttatgact
    12001 ccaggaggaa gttcatcccc tttctttatt ttgttgatct tatcttcgta atagagtttg
    12061 atgatctcaa tctggttttc tttgctatcc aggatttttg atatttttgc ttcaagctca
    12121 ttgtcgttga aatcgacatc ctgcagctta tcaatgctga tatgatcaat tttttccttg
    12181 gtaaatatgt cacccttctt tatggcagtc cttccacggc catctttgat atctgcagtg
    12241 gctgtctttc caagaaaaag actgttcaac ttctctctcg tgctttcaag tattatcttt
    12301 acctgatcct cctgatcctt gaggacattg gaaacctctt tgtcctctat atccctgctc
    12361 cggtcatcct tatcaatgcc ccttctcgat aataccttca catcggtcac aattccttct
    12421 ataccgtggg gaacccgtag ggatgtatct ttcacatcac cggccttttc accaaaaatg
    12481 gcgcgaagca atttttcctc aggtgttaat tgtgtttcac cttttggcgt aacttttccc
    12541 acaaggatat catttggctt gactgtggct ccgatcctta taattccgct ttcatcaaga
    12601 tctttcagcg tctcatctcc cacattgggg atatctctcg ttacctcttc cttacccagc
    12661 tttgtgtcac gaaccacaca ctccagttct tcaatgtgga tagatgtaaa cacatcttct
    12721 ttgacaagcc tttcattgat aaggactgaa tcctcatagt tgtaaccacg ccatggcata
    12781 aaagccacca ggacattctt gccaagagca agctcacccc tgtctgttga aggaccatct
    12841 gcaaggacat cgcctttttt aacaaagtcc ccttccttta cgattaccct ctggttaatg
    12901 cacgtatcct gattcgacct tctgaactta agcagattgt ggacgtcaat cttcgttacc
    12961 ggctcgtcat gtttgctttt ttcctcgaat ttaaggataa tcctgttggc atcaacgctc
    13021 ttgatgactc cgctatgctt agcggtaata gtaaccctgg agtctcttcc cacaaccttt
    13081 tccatccctg ttccaataat gggtgcctct gtaatgatca acgggacagc ctgcctctgc
    13141 atgttcgaac ccataagggc tctgttggca tcatcatgct caaggaatgg tattagagat
    13201 gcagatgcac ttacaagctg cttcggagaa acgtccatgt aatctacctg gtctggaccg
    13261 atgaggtaga agcctccccc tttctgtgct gatacgagcg gttccttgaa tttcccatgt
    13321 tcatcaacag ggctattggc ttgggctata aagaactgtt cttcttcgag ggcactcagg
    13381 tattgcacct catcagtaac catccctttc tcaactttcc ggtaaggcga ttcaataaaa
    13441 ccgtattcat tcacttttgc atatgttgaa agagaagcga tgagtccgat gttgggtcct
    13501 tccggggtct caatcgggca tatcctcccg tagtgggtgg gatgtacgtc ccttacctca
    13561 aaaccagctc tttctctcgt gaggccgcca ggtccaaggg cgctcaaccg tctcttgtgg
    13621 gtaatttcgc tcaatgggtt tgtctgatcc ataaactgtg aaagctggct tgatgcaaag
    13681 aaatccttca cagccgttgc caccggcttt ggattgacaa ggtcatgggg catgagggtc
    13741 tccatctcgg ggaatgtcag cctttcctta atggcctttt ccatacgcac aagacccatt
    13801 ctgaactgat tttccagaag ttcccccact gtccttaccc ttctgttgcc caggtgatct
    13861 atgtcatctc ccgctcccct cccgtctttg agttccagga ggtgtttcac aacctcaatg
    13921 atatcatctc tcctcaatgt ggaaacatca aggggtatat cgagcgacaa ccggtgattt
    13981 attttaagcc tgcccaccct tgaaagatca tagcgttccg ggctgaaaaa catgttgtgg
    14041 ataagtgctt cggcaaattc aatggttggc ggatcacccg gccgaagttt acggtatatt
    14101 tcgaggaggg cgtcttcttt cgtattcacc ttatcagttt caagggtatt cctcaatgaa
    14161 gggctgacgt tgagattgtc tataaagagc acttcgaagg ttttcacctt cttcttggca
    14221 atcgtttcaa gatcctcctt cgttatcagc ttattaatgg gaatcaaggc ttcttttgtc
    14281 tttggatcaa taatctcgga ggatgtaacc ctgccaatta tgtcatcttc ttctacaggt
    14341 atttctttga tgttggctgc ctccatcttt ttgacgtccg ccctgcttat ctttttgtgt
    14401 tttttgatga gaacctcatc ggttttggga ttgagaatat cgtggtgtgc tttttgaccg
    14461 ataaggaggg caaggggggt ctccttgtag agcttgcctt cttttatgat gactctttct
    14521 ttttggtaaa aatagtccag tatctctttc tccgtataac ccaatgcttt cagaaggagg
    14581 gttactggaa ttttcttctt cttatctatg cgaacataca caagttcctt gctgtcaaac
    14641 tcaaaatcaa gccatgaacc tctgtatgga ataattcgag ctgtataggc aagtgtcccg
    14701 cttgtggtag atttggcctg atcagcatca aagtaaactc ctggcgatct atgaagctgg
    14761 ctaacaatga ccctttctgt gccattgatg ataaaagttc ccctttcagt cataagtgga
    14821 atctcgccaa aatacacatc ctgttcctta acagcccgta tatccttggt ttttgttatg
    14881 gaatcgatat tccaaacaac gagtctgatg gtaactttca tcggtgcggc aaaggttaga
    14941 cctctgaaaa ggcactcttc ctcggaattt tttggatcac ccatttcgta tttgacaaat
    15001 tcaagagacg ttgtgtcatg tgagtcttta atgggaaata cacttttgaa aaccgcctgt
    15061 aaaccaatgt tctctctttt ttctggggca atatctctct ggagaaagtt aatatatgaa
    15121 tcaagttgga tttcaatgag gttagggata tcaagaatct ccttgatctt gccgtaattc
    15181 ttcctgaaaa tcttgttatt gaaggtttcc ctcatattgt ttttcacctc atattattcg
    15241 gggttcaaga ggtcaagggt tcgggagtct gtcgagtcag acatagtttg accctttccg
    15301 tcgaaccctt gtaccatcat gtttactcta ctttgacctg gccgctcact tcttcgagtt
    15361 gtttcttgat gtttgctgct tcgtctttcg aaactccctc tttaacaggt tttggcacgc
    15421 cctctacaag gtcttttgct tctttcaagc caagacttgt tatagctctt acaactttaa
    15481 tgacctggat ttttttgtct gcctcatagc cagtaaggat tacgtcaaac tcggttttct
    15541 cttccgctgc ttcaactacc ggagcagcgc ccgcggctgc cggagcagcg cccgcagcca
    15601 taacaggcat tgctgcagta actccaaatt tctcctctaa atcctttata aaatcagata
    15661 gttcaagaac tgtcatgttc tctataaatt gaattacatc ttcttttgta acgctcatgt
    15721 attacctcct ttacccttgt tctttttgaa ttttgattgc attcagcgtc atcattagtt
    15781 tgttcatatt acctgaaagc acataaagga atctttgtgg cataccttgc aacaagccaa
    15841 ggaatttgcc aaccagtaca tcttttgatg gaatcgtggc aagtttcaga atttcttccg
    15901 atgttatgac cctggtgcca agaaatcccc ctttcagttt caggtttggt atatccttag
    15961 caagaccgga taaaacctta gctgcacttg aaggatcctt attgatgcag ataatggcat
    16021 tgggcccttt gaacttatcc tgcaatacct cagcctttgt ccctcctgac gcaatcttaa
    16081 ggagcgtatt tttcacaacg ttgaattcta catcaacatt gctgagctcc cttctcagtt
    16141 ttgtcatctg actcatgttt gtcccgctgt actcagcaag aaacatggca ttgagactgg
    16201 caagttttgc cttcaattcc tcaactatct tttccttctt ttgtcgttcc aatggttttc
    16261 atccctcctt tctgaaaatt tatatggaaa gtcaggaaag cgacgctgta ataaatctcg
    16321 gtaggccgta ttcgtttaat cccgtttttc ggaacaccta ctttctctga cttattccct
    16381 atctgctcaa atttctaaca tatgctgggt caatttttat acccgggctc atggttgtgc
    16441 aaatggcgat gcctttcaca tacgtccctt tgctcgaagg tggtttcaat cgtacgattg
    16501 catcgaggag tgagttaaca ttttcaagga ttttttcttt tccaaagctt attttaccaa
    16561 cagggatatg gaggttacca gccttatcga ctctaaactc taccctccct gccttcatct
    16621 cttttacggt ctttgctaca tcaaatgtga ctgttccaac tttcgggtta ggcataagcc
    16681 ctcgaggacc tagcacttta ccgagtttgc tcaccattgc catcatatca ggtgtcgcaa
    16741 ttgccttatc aaagtccagc cagccttttt gtattttctc gataagctcc tcagctccaa
    16801 agaaatctgc accagcttct tcagcctctt tttctttctg ccccttggca aaaacaagca
    16861 ctctaacctt tttacctaat ccgttcggaa gggcaacact ccctctgacc atctgatcgg
    16921 aatgtcgtgg atcaacacca agccgcagtg cgatttctac agtctcatca aattttgcat
    16981 aggtcatctg gggaagaaga tccagggctt cctccatatc atatctcttt tcgcggtcga
    17041 ccttttttat tgcttcagcg tatttctttg ctattttcgc catgtgtact ccttctataa
    17101 aaacatattg gccatcagca gtacagctct gtcagctgcc agcagtaaat catggatctt
    17161 tgttgctgaa agctgtgggc tgtgctgaaa gcttttttat ccttccacaa cctcaaggcc
    17221 catgcttctg actgagccct caattatttt catagccccc tcgatatttt ttgcattgag
    17281 gtctaccatc ttgagctggg ctatctcttt aacctgtgac ttcgtaatac ttcctatcat
    17341 ctcctttttt gggttgttag aaccttttgc gagttttgcg gctcgtttga taagaaaggc
    17401 agcaggcggt gtcttcgtaa caaaggtaaa agttctatcg gaaaaaatcg tgatcaacac
    17461 cgggatcact gttccttctt gagtttttgt tttttcgttg taagctttgc agaattccat
    17521 gatatttacc ccgtgctggc ctaaggccgg tcccactgga ggtgatggag ttgcctgacc
    17581 tgcttgcaat tgcaatttga ccaaagcaac gacttttttt gccattttct aactccttta
    17641 gattttttct atctgaatga aatcaagttc aactggcgtt gttctcccaa aaatattgag
    17701 cagaaccttt accttgccct tctcgggctt tatctcttcc acattacccg tgaagttagc
    17761 gaatggccct tcggtaacct taacaccatc acctttttcg aacctgattt tgggttttat
    17821 ctttcccttc ccttcctgga ttgcgtaaat gatctcatcc acttcgtggt ctggcagagg
    17881 tggcgggttc atcttatcat atcctacaaa gccggtaacc tttggcgtat tccggaccaa
    17941 ataccatgta tcgttgttca tgaccatatt cactataatg taacccggga acacagtacg
    18001 ttgtgatttt tttacctgcc ccttcacttt ttccataatg atttcagatg gtacaattat
    18061 atccccgaaa aactcttcca tcttgacgtt tttgatgttt tcttcgaggt tctctttgac
    18121 tttctgttca tagccagaat acgtgtgtac cacataccat ttcttttcca tctgattatc
    18181 tctttatttt gcctgattat cctcttaaaa ttgcctgaat cagtcgagcc aaccccacat
    18241 caacaatacc aagaaatatg gctgatataa aaacagttat taacacaata taggtgccct
    18301 tcagggcgtc ttttcttgaa ggccatgtga ttcttttccc ttctagatac acttctctga
    18361 aatatgcctt tacaacctca atcttctctt tgacatccat ggcatctcca tatttttcag
    18421 gccaggaggg attcgaaccc ccaacacccg gatttggagt ccggtgctct atccgttaga
    18481 gctactggcc ttttattttg tctctctatg cggggtatgc ctcttgcaaa atctgcagaa
    18541 cttctttatt tcaagtctgc ctggtgtttt ttgtttgttt tttgttgttg tataatttct
    18601 attcttgcac tctgtgcatg caagcgttac aagctgcctc atcttccatc ccctctattc
    18661 tatgatctcg gtgcataccc cggcaccta
//
LOCUS       DAOOMC010000051        18573 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdus sp. isolate AMR_MDS_2685
            NODE_3057_length_18573_cov_5.839021, whole genome shotgun sequence.
ACCESSION   DAOOMC010000051 DAOOMC010000000
VERSION     DAOOMC010000051.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864760
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdus sp. (wastewater metagenome)
  ORGANISM  Syntrophorhabdus sp.
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae; Syntrophorhabdus.
REFERENCE   1  (bases 1 to 18573)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18573)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,729
            Genes (coding)                    :: 2,708
            CDSs (with protein)               :: 2,708
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 7 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18573
                     /organism="Syntrophorhabdus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3057_length_18573_cov_5.839021"
                     /isolate="AMR_MDS_2685"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2699756"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..260
                     /locus_tag="PK125_06745"
     CDS             <1..260
                     /locus_tag="PK125_06745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006779552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TCP-1/cpn60 chaperonin family protein"
                     /protein_id="HPB37840.1"
                     /translation="IVVEKVKNGKGNFGFDAAKEEYVDDIMDAGIIDPTKVARTALQN
                     ASSVAALLLTTDAMIAEKPKEKGAGMPMMPPGGMGGMGDMY"
     gene            complement(298..519)
                     /locus_tag="PK125_06750"
     CDS             complement(298..519)
                     /locus_tag="PK125_06750"
                     /inference="COORDINATES: protein motif:HMM:NF018961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dodecin family protein"
                     /protein_id="HPB37841.1"
                     /translation="MAGSVYKIIEIVGTSTKSWEDAAKAALETASKTLEDLRVAEVVK
                     QDVTVENGKVINYRVRLNISFKYHTEKGL"
     gene            complement(613..1221)
                     /locus_tag="PK125_06755"
     CDS             complement(613..1221)
                     /locus_tag="PK125_06755"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: protein motif:HMM:NF016107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HPB37842.1"
                     /translation="MLDTIALLVLVLWPIIPLWWIPVHGANKIVRKMGFFVCPVIGIL
                     WVTIAYIIYTNRVFFLGFNIDFFILIRAGGILCAGAGVFLQLWTLRVLNFLVITGAPE
                     IFDGVKAKLSVHGPFSYIRHPTYLSHTMFFLGVFLITGNLAIGLVALIDFLTMNFIVI
                     PLEEKELLQRFGIEYKSYGPCSPFYSAIEQITIVCRKTISQY"
     gene            complement(1259..2182)
                     /locus_tag="PK125_06760"
     CDS             complement(1259..2182)
                     /locus_tag="PK125_06760"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HPB37843.1"
                     /translation="MKHDFRKVIGLKVDVDTYEGMKNGVPKLLDCLNQFNIKASFFVP
                     MGRDHTGWTVKRVFTRRGFLKKANRVGVLETYGMRTLMYGLLLPGPKIAEKNRSVLRE
                     IVEQGHEAGIHGYDHVIWHDRVKKWDDVKTSAILIRACRKYEDILGVKAHSFAAPGWM
                     INPYALQFFENNGFLYSSDTRGVSPFLPIMGDRPINILQIPTTLPTLDEVVGIAGSEP
                     HLLAGYFKDLLSENLNILTIHTELEGKRWLGFLMDFIRLANEQGFTFMRLTDIAQMLK
                     GKNSIPRCKIFYGHVEGRAGEVSCQKPSDLS"
     gene            complement(2247..3887)
                     /locus_tag="PK125_06765"
     CDS             complement(2247..3887)
                     /locus_tag="PK125_06765"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR02504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin-dependent
                     ribonucleoside-diphosphate reductase"
                     /protein_id="HPB37844.1"
                     /translation="MVDDNALLVLKKRYLIKDVRGNAVETPDQMFERVVGFVAGAEEM
                     YGDKGREEIGYDFFHMLRNLEFLPNSPTLMNAGRELGQLAACFVLPIEDSLDSIFDQV
                     KMTAKIHQSGGGTGFSFSKLRPKDDVVASTMGVASGPVSFMTVFNMVTDVIKQGGTRR
                     GANMGILRIDHPDIEEFITIKNNPEAMNNFNLSVSVTDEFMAAYEKGIRFPLINPRTK
                     EETKTVDPRELIQMIAQSAWKSGEPGMIFIDTINRENPTPHIGAIEATNPCGEQPLLP
                     YESCCLGSINLAKLVKNGEIDWRRLRELTHLGVRFLDNLIDINRYPAPQIEDISRRNR
                     KIGLGVMGFAHLLIKLGIAYDSFDAVKTAEEIMAFIQAESKVASNLLAKQRGVFPSYK
                     GSVWEARGLRQRNATTTTIAPTGTLSLIAGTSGGIEPIYDVRYSRVLFGNIKVDIVDP
                     LYRNAREKDNNEEQMKKIFRRAHDVAPSDHLAIQSAFQKHVDNAVSKTINLPQDATID
                     TVVDVYLRAHRMGLKGVTIFRDKSRGAQVLSCGTQQAC"
     gene            complement(3902..4387)
                     /locus_tag="PK125_06770"
     CDS             complement(3902..4387)
                     /locus_tag="PK125_06770"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013756.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HPB37845.1"
                     /translation="MSTKNTKVSRIMPCSVNLLTVASKTEREAMTATAMFISEDPPLF
                     VVSVQKHILTHKIIEETGQFVLNIPSTEQVRLAKRLGHEKSPDKFKEFDIETESAKAV
                     SAPLIKGSFASIECKVITSFPASNFTVYVVEAVDFAFNDNLSPVVWHLNRYYSLGDEL
                     K"
     gene            4567..5445
                     /locus_tag="PK125_06775"
     CDS             4567..5445
                     /locus_tag="PK125_06775"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HPB37846.1"
                     /translation="MTNPWILWALLSAFMAASNDALTKKALSVHNEYLIAWLRLLWAL
                     PFLIVTLAFIPIPLIDSIFFLAFLVALPLEVTATVLYVKALKTSPLSLTVPFLSLTPI
                     FLIVVPYLILGETISVLGVIGVAMIAFGGYTLNIGQARKGLLAPFFAVRKERGSLFMI
                     GVALIYSVTATLGKLAIQHSSPLFFGITYYSALACAMTPIALLRGRDERKYVPPRDAI
                     KASILPGFCHATSIVAHMIAISLTQVAYMISIKRLSLLMGVLFGYLFFKETNIRERFV
                     GTLLMIIGFALIVLFH"
     gene            5567..6136
                     /locus_tag="PK125_06780"
     CDS             5567..6136
                     /locus_tag="PK125_06780"
                     /inference="COORDINATES: protein motif:HMM:NF019325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CARDB domain-containing protein"
                     /protein_id="HPB37847.1"
                     /translation="MHSLLTKSVFIIMLLSSAFGNLYAASTLVFTTVACSPATVTLES
                     KFSLTTTMVNQGDAASPTTVTNYYLSIVTSKEGTWLLTSKHSVKGLAPLANTTKKVKI
                     TVPSGTYYLVACADGVNKVKEEGGTNNCLNSQPTSSSVSSPSLFTLIPENLFPSSRDN
                     NPCLHRAHQHQNTLFLPTHHTPCILFSLL"
     gene            6755..7957
                     /locus_tag="PK125_06785"
     CDS             6755..7957
                     /locus_tag="PK125_06785"
                     /inference="COORDINATES: protein motif:HMM:NF014164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HPB37848.1"
                     /translation="MVLTTAELEKYADVLLWGLKTARKSKFKKGDIILIQYENPALPL
                     AEILFKKIVAIGMHPVQRMGLTFGMEKSFFKEADDKQLVFIPPGEKELYENVNGRIFL
                     RAPESLTHLKDIDPARIGTVLVSRKPLKDILDKREEQGFYSWTLCTFPTHELAWQAKT
                     TIRHYAAQIIKACYLDKENPVQEWESILNNVHGIKKWLNSLKIKTLHIEAKNIDLTIT
                     PGEKRKWSGVSGHNIPSFEIFFSPDWRGTEGTYYANLPSFRSGNYVKGIRLTFQKGAV
                     VKIEADEGEQFAIKQLAMDKGASRIGEFSLTDRRFSRIDRFMADTLFDENFGGRHGNS
                     HIAVGSSYTESYTGNQADMTKQLKEKLGFNDSALHWDLVNTERKTVTAHLTSGKRLVI
                     YEDGQFKV"
     gene            complement(8503..9270)
                     /gene="hisF"
                     /locus_tag="PK125_06790"
     CDS             complement(8503..9270)
                     /gene="hisF"
                     /locus_tag="PK125_06790"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR00735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HPB37849.1"
                     /translation="MDFVKIMPCLDMKNGKVVKGVHFVDLKEAGDPVQNAAFYQKEGA
                     DELAMLDIAATIENRKTRLDWVKQVSSVISIPLTVGGGINSLEDIQLVLDAGANKISM
                     NSAAVHNPGLIKEATDRFGSESITIAIDARRNAALASGFELVVSGGTKPTGKDAVEWA
                     KVCEGLGAGVILPTSMDGDGTLAGYDLEFTRAISDAVNVPVVASGGAGTLEHFYEGVT
                     KGGAQILLAASVFHYRTLSIKQVKTYLFEKGLKVTFH"
     gene            9879..10478
                     /locus_tag="PK125_06795"
     CDS             9879..10478
                     /locus_tag="PK125_06795"
                     /inference="COORDINATES: protein motif:HMM:NF024139.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HPB37850.1"
                     /translation="MDKICIVKRRIQSPTLLDHSFFCEEKSMDKTQELADPHSFVPER
                     FAPDGNGKQHIYPFRGAVRDGDNIIRIQLTPDQCTVIQAKGALNCFIGKILGSIDVDF
                     EGYEDGQIVFNLHLKHVERTDMLDSKNVCKMLQVSKSFLSRLVKAEQIRSYKMGRLRR
                     FLLEDVLDYLSRSEVLQKARECTPSKTKKVLRQGLADSR"
     gene            10499..11791
                     /locus_tag="PK125_06800"
     CDS             10499..11791
                     /locus_tag="PK125_06800"
                     /inference="COORDINATES: protein motif:HMM:NF024793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HPB37851.1"
                     /translation="MYFDYWGLKKPPFDNVPDPSMYVASHTSVENTIAETLFAIEEGN
                     ECIAVIVGDVGLGKTMSLRMVIDSLEPSKYKIAFITNPDIPFIQLLREIIGQLTGRQC
                     EEKRKVDLLEIFNKLLFQTADEGRKVLIFIDEANAMSPANLESLRLLTNMQDDNRNLF
                     TIVLAGQIELARRLEHPRRANLFQRIGTYCNIERIESEELVKTYVETRLALAGATKKI
                     FTDDAITAIWIYSENGVPRLINKICKLCMKAGETNNLQEIDGNIVKQISERFQKLTGP
                     AMPKRKPRLRSSDDKNIDEPGEAILEEQVPVEPPKVEQNVVMMKEEAIQPEPALDPQP
                     PLPEAAPCESKEDGEEVSIGKYGINVNIPPHIIEQAQSFTDEHRSKLAGVLAAQTMQK
                     HPQLTGTADMDPVTIWSDIRECILLKLKQEPGKAFVQG"
     gene            complement(11879..12790)
                     /locus_tag="PK125_06805"
     CDS             complement(11879..12790)
                     /locus_tag="PK125_06805"
                     /inference="COORDINATES: protein motif:HMM:NF024816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPB37852.1"
                     /translation="MQWLKTDKRKSAKAWFIKGCWYDSDPSQTENAIAAYRESIKIDN
                     TYTDAFVNLGFIYLRKEEYEEALQCFQRVAELETTKPEAYNNLGYVYEKMERFGSAKK
                     MYERTLEFDPNDVEAMINIAHIDDLEGNYHSAVIQYKKAIEVAPNEVAPHFCLAVLYD
                     QHDMFDEAIGEYEATLEINPYHLKALFNLGNLFAQEGRYKEAIMCYKRALVLDGDDIS
                     AWNNLGAIYEDMNELSRAESIYKRAQALNPYKAETNFNLARIQLLQHQKDLSEEMRQD
                     IIRRLNFVLSVNPHNKSAERLLTGLLK"
     gene            complement(12936..14099)
                     /locus_tag="PK125_06810"
     CDS             complement(12936..14099)
                     /locus_tag="PK125_06810"
                     /inference="COORDINATES: protein motif:HMM:NF021502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2226 domain-containing protein"
                     /protein_id="HPB37853.1"
                     /translation="MRYPKGEVVHQNLSTEYTDIPELLATLSSEGFTGVVEVVLAKET
                     GAFIVMEGKALGALIESEPDQSTTFSQEAMDELIGIASKQAGILNIYKLSPAQVDAIV
                     SRFSAEIVFKGLTTDFVKLDKFIQKLGAEHHTGYIEVFTKKGQPMGTLFLKEGELIDL
                     HLRSGLESPSLSEPKAIPTFLEDVIHQGAVFDVYRSLVTNISSETHGDTKSIGQVIKN
                     EDSPATIIDAETIQELAEVEQTKEIAGESINSHERLNSNGHKQILAALQEIIARVEKF
                     VDDFSQEGIFLRAFKRALIEKSDTYPFLDPFTEQFDYRHGEITLDEEVELEDFAAGIA
                     DCFNLTVSHLKKEFPKNMNLSLNTKTELESKFKLYQEAMMQSGVQSVPPMLFK"
     gene            complement(14134..15111)
                     /locus_tag="PK125_06815"
     CDS             complement(14134..15111)
                     /locus_tag="PK125_06815"
                     /inference="COORDINATES: protein motif:HMM:NF028510.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HPB37854.1"
                     /translation="MANDTKGGMGLLPRLIIGIFIPILLAFLIISSILFFSINIGLFQ
                     FKSIRDIGFSSLSDLSTAAVKESTTSLNKLGERIIYEKAQDVAKQIEIFVKSQPNTSL
                     AVLSKNSRLKEIAIQKVGETGYTAVHDNRGINYFHVNPQIVGTDLHQLATKLPEFVKI
                     LDAGLAGPASGYYNWKDADGSVRPKFMVNIPVRGTNLVVAATTYIDEFSKPAKNIVDK
                     MSQIEKAYSSQYNNKFVIFYIIVLIDLLVLLVVIYLYSHSIIRPIRYLSDVADKISMG
                     DLKTTIKVKAKGEVAVLAESIERMQTSVKAAIDRLQKRRDNGKPQAGNG"
     gene            complement(15126..15941)
                     /locus_tag="PK125_06820"
     CDS             complement(15126..15941)
                     /locus_tag="PK125_06820"
                     /inference="COORDINATES: protein motif:HMM:NF025690.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4388 domain-containing protein"
                     /protein_id="HPB37855.1"
                     /translation="MPSYFIGELSETRLFDLVKPLLDGKKSGMISIKGREYSEIHIEG
                     GKIIHAWTNYNTGEEAILAMMEWRVGRVSFDWEVTTEERTVNESTEQLLASWKNREEE
                     WKIIRESVPSPGVMFRIAMQDGVEDRSITGDQWKVLALCNGSKRVSDIAAALQWDMYK
                     TSNTIFQMVRGGLLERAGEAPEEQKPLQKKTVGEGFFPMVENALKKLMGPIAPIIIDD
                     IIAEIGESREAFPQEMVDKLLEAIVSEIPDMAKRAEFTKVVHNALANDHKMKS"
     gene            complement(16119..17684)
                     /locus_tag="PK125_06825"
     CDS             complement(16119..17684)
                     /locus_tag="PK125_06825"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HPB37856.1"
                     /translation="MIRHNGILLIHPPVVKPCEPPAGISKLSGALTAHGINHSVLDLN
                     LECLLAVLSGQAKTPSDTWTLRAQRNLHQNISILRSWKGYGSTERYRSIVTDLHRIFQ
                     VHGGSQIKRLSFSDYQHIDLSPLRSADLLKAAEEYKQNFFYPYFRDSLRAALEKTGTD
                     LVGLSLNYLSQALCTFAIIGFIKETMPDVQVVLGGSLVTSWIRVNVWKNQFQGIVDHA
                     VAGPGEYPLLALLGCTTEKNQQYCPSFHDFPLGEYLAPGPILPYNASTGCYWSKCAFC
                     PENAEGSVYTGRSKNIIAKELQSLSSELQPVLIHFVDNAISPVVMAGLCSNSPGVSWY
                     GFARLTNHLASLDFCRDLRRSGCVMLKLGLESGSQAVLNAMNKGNNLERSSRALKSLN
                     KAGIATYVYLLFGTPWETEVEAQETLEFVVRHSPYIDFLNMAVFNLPNNSPEATQLNT
                     RDFYVGDLSLYTGFSHPSGWDRKSVKIFLDKKFRRHPAIQSILRRHPPGFGSNHAPLF
                     SMRNEGKPIPKNS"
     gene            17811..>18573
                     /locus_tag="PK125_06830"
     CDS             17811..>18573
                     /locus_tag="PK125_06830"
                     /inference="COORDINATES: protein motif:HMM:NF022995.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protoglobin domain-containing protein"
                     /protein_id="HPB37857.1"
                     /translation="MSDNVTPLHKLRIFKEQIGIDAADLAELAPFRHFFTERKEEFGD
                     YFYNVFWGIPATRTILEGEKTRGVLRKTWSLWFETFFSADFDDTFLSWLWKIGVRHVE
                     VNLDQRFSNLGFAVIRQFCHGIVFKELPLQQRGRVLSIIDKLLDLCVLVETSAYIENT
                     ISCNIEVMREMADRVRNPAMIIGWNIKRLHDKADADSKEYRVYKMLMEENERLEGMVR
                     DIKVYMDLFETEPEIDTVNFEEIINEVLLKLKEEGT"
BASE COUNT         5296 a         4190 c         3845 g         5242 t
ORIGIN      
        1 ccatcgtggt agaaaaggtg aagaacggaa agggtaattt cggatttgat gcagcaaaag
       61 aggagtatgt ggatgatatt atggacgccg gtatcataga ccccacaaag gttgcacgaa
      121 cagcccttca gaacgcttca tccgttgcgg ccttgttgct cacaactgat gcaatgatag
      181 cagaaaaacc gaaagagaaa ggcgcgggaa tgccaatgat gccgccaggt ggtatgggtg
      241 gtatgggaga tatgtactaa taaaaaagag gggaccaaag tcccctcttt ttttctttta
      301 tagacctttt tcagtgtgat atttaaaaga gatgttcaat cttacacgat agtttattac
      361 cttaccgttt tccacagtta catcctgttt tacaacttca gcgactctca gatcttcaag
      421 ggtttttgaa gcagtttcca gtgcagcttt tgctgcatct tcccatgatt ttgtgctcgt
      481 tccgactatc tcgatgattt tgtatacact tcctgccata aataccctcc ttatttgttt
      541 atttttaact ctatatacgg ttatttatac catagataaa caagttgtca agcttgtttt
      601 aaagaattac cgttaatatt gggatatcgt tttccgacag actatggtta tctgttcaat
      661 cgcggaataa aacggggaac acgggccata ggacttgtac tctataccaa aacgttgcag
      721 gagttccttc tcttccaaag ggataacaat aaaattcata gttaaaaaat caatcagggc
      781 aacaagacct attgccaagt tgccagttat taaaaaaacc ccaagaaaaa acattgtatg
      841 cgacaggtat gtaggatgcc ggatataaga aaaagggcca tgaacactga gctttgcttt
      901 tactccatcg aatatctctg gagctcctgt aataaccagg aaattgagta cccttaatgt
      961 ccatagttgg aggaatacac cggcgccagc gcaaagaatc cctcctgctc tgataagtat
     1021 gaagaaatct atgttaaacc ctaagaaaaa taccctgttt gtgtatatga tgtatgctat
     1081 agtaacccat aatataccaa taaccggaca aacaaagaat cccatcttcc tcactatttt
     1141 atttgcgcca tgtacgggta tccaccacag tggaataatt ggccagagaa cgaggacgag
     1201 caaggcaatg gtgtccaaca ttgctaacaa tgtgtaataa tccgtaataa actgtaattt
     1261 aagatagatc ggaaggtttt tggcacgaga cctcgcctgc tctgccctca acatgtccgt
     1321 aaaagatttt gcatcttggg atgctgtttt tccctttgag catttgtgca atatctgtga
     1381 gcctcatgaa agtaaaaccc tgttcattcg cgagacgaat aaaatccata aggaaaccca
     1441 accaccgctt accttcaagc tcagtatgta ttgtaagaat attcaggttc tcactcaaca
     1501 aatctttaaa atacccagca agcaggtggg gctcagaacc tgcaatgccc actacctcat
     1561 caagcgtggg aagtgtggtt ggtatctgaa gtatattaat aggtctgtcc cccataatcg
     1621 gcaagaaggg cgatacgccc cgcgtatcgc tagaatagag aaatccatta ttttcaaaaa
     1681 attgcagcgc ataagggttg atcatccacc cgggagcggc aaaagaatgg gcttttacgc
     1741 cgagaatatc ctcatatttt ctgcaggcac gaatcagtat agctgatgtt tttacgtcat
     1801 cccatttttt gacacggtca tgccagataa catgatcata accatggata cctgcttcgt
     1861 gtccttgttc aactatttct ctgagaaccg acctgttttt ttcagctatc ttgggcccgg
     1921 gaagtaaaag accatacatg agagtccgca taccatacgt ttctaatact cccaccctgt
     1981 ttgctttttt taaaaatcct cttctggtga aaacccgttt gacagtccaa cccgtatgat
     2041 ctctccccat gggcacaaaa aaactagcct tgatattgaa ctggttcaaa cagtcaagga
     2101 gttttggcac tccgttcttc atgccttcat acgtatcgac atccactttt aacccaataa
     2161 ccttgcggaa atcatgcttc atgctatgat ggtatcactt cttaaccggt ttttccaagc
     2221 agattttgac aaaaagcggg cggttattag catgcttgct gggttccaca ggacaggact
     2281 tgtgcgcccc tgcttttgtc tctaaagata gtgacccctt ttaggcccat cctgtgtgcc
     2341 ctgaggtaaa catctactac cgtatcaata gtggcatcct gaggaagatt gatggtcttt
     2401 gagactgcat tgtcaacgtg cttttgaaaa gcactctgaa tggccaggtg atctgatggt
     2461 gctacatcat gagcccttct gaatattttt ttcatttgtt cttcattgtt atccttttcc
     2521 ctagcattcc tgtaaagtgg gtctacaatg tctaccttga tgttgccgaa aagaacgcgg
     2581 gaatatctga catcgtaaat gggttcaatc ccgcctgatg ttcctgcaat caagctcagg
     2641 gtaccagttg gcgcaatggt agtagttgtg gcatttctct gtcgcaaccc tctggcttcc
     2701 cacacacttc ctttataact cgggaatacc cctcgttgtt ttgcgagcag atttgaagca
     2761 acctttgatt cagcctggat aaacgccatg atttcttcag ctgtttttac ggcatcaaac
     2821 gagtcatagg caattcccag ttttatcaac aaatgggcaa agcccatcac tccgaggccg
     2881 atttttcggt tccttctgct aatatcttcg atctgcggtg cagggtacct gtttatatca
     2941 atcagattgt cgagaaaccg gacgcccagg tgggttagct ccctcagtcg tctccagtcg
     3001 atttcaccat tttttaccaa cttggcaagg ttaatcgatc ctagacagca cgattcatat
     3061 ggaagaagcg gttgttcacc gcaaggattg gtcgcctcga tagcgccgat atgaggcgtt
     3121 gggttttctc tattgatggt atcaatgaat atcatcccag gttctcccga cttccaggcc
     3181 gattgagcga tcatttggat cagttctcgt ggatcgacag tttttgtctc ctcctttgtc
     3241 ctcggattga tgaggggaaa cctgatacct ttttcatatg ctgccataaa ttcatcagtc
     3301 accgagacag aaaggttgaa gttgttcatt gcttcaggat tattcttaat agtgataaac
     3361 tcctcaatat caggatggtc aatcctgagg atacccatgt ttgctcctct ccgggttccg
     3421 ccctgtttta taacgtctgt aaccatatta aaaactgtca taaaggatac gggaccgctt
     3481 gcaacaccca ttgttgaagc cacaacatcg tctttaggcc ttagtttcga aaaagaaaac
     3541 cctgtcccac ctccagattg atgaatcttt gcagtcatct tcacctgatc gaagatcgaa
     3601 tcgagcgagt cttcaatggg aagaacaaag caggcagcaa gctggccaag ctccctccct
     3661 gcgttcatca gagtggggga gtttgggaga aactcaaggt tgcgaagcat atggaagaag
     3721 tcatacccta tctcttcgcg ccccttgtca ccatacatct cctcagcccc tgcaacaaaa
     3781 ccgacaaccc tctcaaacat ttggtcaggg gtttccacag cattaccacg gacatccttg
     3841 atgagatatc gttttttcaa aactaaaagg gcgttgtcat caaccatgga tctccgctaa
     3901 tctatttcaa ttcatctccc agggaatagt acctgttcaa atgccataca acaggcgata
     3961 aattatcgtt aaaggcgaaa tcaacagcct ctacaacata gactgtgaaa ttacttgcag
     4021 ggaaagatgt aataacctta cactcaatgc tggcgaaaga tcctttgatc aatggtgcgc
     4081 taactgcttt agcgctctcc gtttcaatat caaattcctt gaacttgtca ggcgatttct
     4141 catgtccaag ccttttagcc agtctaacct gctccgtgga tgggatgttt agaacaaatt
     4201 ggccggtttc ttcaataatt ttatgagtca agatatgctt ctgaacactt acaacaaaga
     4261 gaggaggatc ttcagaaata aacattgcag tggcagtcat tgcttcgcgc tctgtctttg
     4321 atgctactgt cagcaggttg actgaacatg gcataatacg acttactttc gtgtttttgg
     4381 tcgacataat aacccccctt aatatgtttt atggcagtat cttcaaaaag acacttttgt
     4441 ataacataat caattccaac cgcatgtcaa tgagatgaaa tggaaaaaag tagttcaact
     4501 atttgagatg aaaaaacacc taaaattaaa ttgcttaata gtaattaaag gctttacaat
     4561 gcattcatga caaatccatg gatactgtgg gcactcctgt ccgcatttat ggctgcatca
     4621 aatgacgccc tcaccaagaa agcgctttcc gttcacaacg aatatttgat tgcctggctc
     4681 aggcttctct gggctctccc ctttctcatt gtcactcttg cattcatccc gataccattg
     4741 atcgatagca tatttttcct ggcattcttg gttgcactgc cccttgaagt gacagccact
     4801 gtcctttacg ttaaggccct gaaaacatct cccctgagcc tcaccgtacc ttttctttcc
     4861 ctcactccta tttttctcat agttgttccc tatttgatct tgggagaaac tatatctgtt
     4921 cttggcgtaa ttggtgtagc aatgattgcc tttggaggtt ataccttgaa tataggtcag
     4981 gcaagaaagg ggcttcttgc ccctttcttc gctgtcagga aagagcgggg ctctcttttt
     5041 atgataggcg tggctctcat ttacagcgtc acagcgaccc ttggaaagtt ggcgattcag
     5101 cactcctccc ctctcttttt tgggataacg tattacagcg cccttgcttg tgccatgacg
     5161 cccatcgcgc tcttaagagg ccgtgatgaa agaaagtacg tacctcccag agatgcaatc
     5221 aaggcatcca ttcttcctgg attctgtcat gccacgagca tcgttgccca catgatcgcc
     5281 atcagcctca cccaggtagc ctacatgatt tcgataaaac gattaagcct cctcatgggt
     5341 gttctcttcg gatacctgtt tttcaaggaa accaatatca gggaaaggtt tgtgggcacc
     5401 cttctcatga tcatcggctt tgctctcatc gtgctgtttc attaaaaaaa tagcaatgaa
     5461 tgaaaaataa aattcatttc ccctcttcgc cctttttccg tgtattctgc aaatgtactc
     5521 tgaacagtca ccatactgtt taaccctaac aaggaggaaa atggtcatgc attctctcct
     5581 tacaaaaagc gtattcatca taatgctcct ctcttccgct ttcggtaatc tctacgccgc
     5641 ttcgaccctt gtattcacca ccgttgcctg ctctccggca acggttactc tggaaagtaa
     5701 gttctccctc accaccacca tggtaaacca gggcgatgca gcatccccca cgacagtgac
     5761 caactactat ctgtccattg tcacctcaaa ggaagggacc tggcttctta ccagcaaaca
     5821 ctccgtcaag ggccttgctc ccctggcaaa caccaccaag aaggtgaaga tcaccgtccc
     5881 ctccggcacc tactaccttg tagcctgtgc cgatggcgta aacaaggtca aagaagaagg
     5941 tgggacgaac aactgcctta actcccaacc cacatcatcg tcagtatcat ctccttctct
     6001 ttttacacta ataccagaaa atctattccc ttcctcccgg gacaacaacc cttgcctcca
     6061 tagggcccat cagcatcaaa acactctctt ccttcccact caccataccc cttgcattct
     6121 cttttccctc ctttgatacc tataaaacgg atatggggat agataccccg aacacctgat
     6181 ctatttcggt gatgaccaga ggaaccgcat cgttcagcat gggggatcag ccgatgaccc
     6241 tctctctgtc gataccagag tcgagaacgc tggatagagc agtctggagg gggcggtgta
     6301 aaccaaccga atatagtcgc ttgaactgga aatgactaca tttatcagta accggttcgg
     6361 gtggcggaac aaccatcaca gtgaccaaca tcgagcagat caccgtcaac gatgcaggag
     6421 ggaaccccat tttcacctgg ccctaaccat agccatggca ggccgtgatg tttctatacc
     6481 ctccatcata ttaaagtaaa aaccttgtca gtcaggttgt ggtcgggtta tactactctt
     6541 aacttgggca ccttatggga gcacagtgtc ggacattctt ttagcaaacc gtcgtgaggc
     6601 aaggaagcct ggtctttatc gtatgtaaaa gttccgcagt cggaggtgag cgaaattctc
     6661 cgctgtagca aatgagcgtg tttgcgggga ttccctgaca acaatacact acgaataaaa
     6721 aagaggttta acgaagatca accgaggggg atgcatggta ctgacaaccg cagaactgga
     6781 gaaatacgca gatgttttgc tatggggcct aaaaacagcc cgtaaatcaa aattcaaaaa
     6841 gggagatatc atcctcatcc agtatgaaaa ccctgcactg ccccttgccg agattctctt
     6901 taaaaagatc gttgccatag gcatgcaccc tgtacagcgg atgggcctta cctttggtat
     6961 ggaaaagagt ttctttaaag aagcagatga caaacagctt gtctttattc caccaggcga
     7021 aaaggagctc tatgaaaatg taaacggacg gatatttctt cgagccccgg aatcactcac
     7081 ccacctgaaa gacattgatc cggcgcgaat cggcacggta ctcgtgtcac gaaaaccgct
     7141 aaaggacatt ctcgataaga gagaggaaca gggattctac agctggactt tgtgcacctt
     7201 tccaacccat gaactggcct ggcaggcaaa aacgactatc agacattatg cagcccagat
     7261 aataaaggca tgctatctcg ataaagaaaa tcctgttcag gaatgggaat cgatccttaa
     7321 caacgtgcat ggcattaaga aatggctgaa cagcctcaag atcaaaaccc ttcatataga
     7381 ggctaagaac atcgacttga ctatcacgcc cggagaaaaa agaaaatggt caggcgtctc
     7441 aggccacaac atcccgagct ttgaaatctt tttctcccct gattggcggg gcacagaagg
     7501 aacctactat gccaatcttc cctctttcag aagcggcaat tatgtgaaag ggatccgtct
     7561 cacctttcaa aagggggctg ttgttaaaat agaggcggac gagggtgagc agttcgccat
     7621 caagcagctt gccatggaca agggtgcatc ccgaatcgga gaattctcat tgactgacag
     7681 gcgtttttca cggattgacc gttttatggc cgacaccctg tttgatgaga attttggcgg
     7741 aagacatggc aactcccaca tagccgtggg atcgtcatac accgaatctt acaccggtaa
     7801 tcaggctgac atgaccaagc agcttaagga aaagctgggt tttaatgact cggccctcca
     7861 ctgggacctg gtcaacactg agcggaaaac agtaactgcc catcttacat cgggtaaaag
     7921 gcttgtcatc tacgaagacg gacagttcaa ggtctgatcc aaaaggtcat gagaaaaata
     7981 ggcttaatgc taaaaaagag atctaaaata gccacacaat accacctcag gagaagcact
     8041 cgtcaattac ctcaaaaaaa tccgcaaaaa ccctgatccc tattagccca gcatcagtgt
     8101 ccgttcatga gaaagtagta tagtttccag aaagcgattt ttacgatctt aaaaactttt
     8161 aagaggtgtt gtgctggagg catggaaaac ttgacagagc caaatcacat aagaattctt
     8221 aaatcagaaa aaaggcttac tccaaagaca ttacaccaaa tgagcctatt ggcaggaaaa
     8281 ccgtgtgaat gttaacaaaa aaatattatt gtgaatctat tcatatcggg gaaattatca
     8341 gatgtcttgt gctattaatg cccatcaggc acaaaaaata aaacaggtgt cccgggcttc
     8401 actgcatacg gttccggcaa cctctctggt ttgtacagaa gcacagggtt caccgatatc
     8461 tggtcgtaac tcttataaag atatcgtttt agtccccata ttttagtgaa atgtcacctt
     8521 aagccctttt tcaaacaaat atgtcttgac ctgtttaatg ctgagagttc gataatgaaa
     8581 gactgatgca gcgagtagga tttgtgctcc ccctttcgta accccttcat aaaaatgttc
     8641 gagagtccct gcgccaccgg atgcaaccac aggaacattg accgcatcag aaatggccct
     8701 ggtaaattca agatcataac cggcaagggt tccatcgcca tccatgcttg ttggtaaaat
     8761 aactccagca ccaagaccct cacatacctt tgcccattct accgcatctt ttccagttgg
     8821 ctttgtccct cccgacacaa caagttcaaa accagacgcc agggcagcat ttctccttgc
     8881 atctatagca atagtaatgc tctctgatcc gaagcgatct gttgcttctt ttataagacc
     8941 gggattgtgc actgcagcgc tattcatcga aatcttgttt gcccctgcat caagcacgag
     9001 ttgaatatct tcgaggctat ttatcccccc tccaacagtg agaggtatgc taatgactga
     9061 agaaacctgt ttaacccaat caagccttgt ctttcggttt tcgatagtag ccgctatatc
     9121 aagcatggca agttcatcgg caccttcctt ttggtaaaag gcagcattct gtacagggtc
     9181 accagcttct ttcaagtcta caaagtgtac gcctttcaca actttaccat tcttcatgtc
     9241 aaggcaaggc ataatcttca caaaatccat gttccctcct tttcagataa acgaggatga
     9301 gtcaatatta tcagatgttt gacgtccaca agtcaagaca ataacatatg aagtttccag
     9361 ggttttttgt tccagcctgc tcttccccag aaggcgaagc atagcttaaa gccaggtatc
     9421 tttttttaat tgtatgtgat tgataccggt tttctacttc gagcctgaga ggtctttaac
     9481 catgatgcac cagagttctg cgacctgtct tatggcagca acccgctcag agaataacat
     9541 agtcagggtc ttttctttgc cagtgttcat accggactga caatgagacg ttgagagatt
     9601 ttcaataaaa acctggtgtg acctgtcaga acagtgattc atcgccaatt accatgtatt
     9661 acctatggct gtgttggaaa attgaggaaa tccaggaaga ttaaggcctg aatctttgac
     9721 ctgagttttc cgtgtgactc acatcattga ccttaattgg cacactttat ataaaacctg
     9781 aaggtttaac aatgtgtgta gacagttatt ggcattaaca aaaaatactt aaggagaaaa
     9841 caggtaagga cgataataca ggcatagagg acgtatttat ggacaaaata tgcattgtaa
     9901 aaagaagaat ccaaagccct acattgttgg atcattcgtt tttctgtgaa gaaaaaagca
     9961 tggataaaac acaggaatta gctgacccgc attcctttgt acccgaaaga tttgccccgg
    10021 atggaaatgg aaagcaacat atttatccat ttagaggcgc agtaagagat ggtgacaata
    10081 ttatcagaat tcaattaact ccagaccaat gtacagttat ccaggcaaag ggcgctctca
    10141 actgttttat tggaaaaatc cttggcagca ttgacgtcga cttcgagggg tatgaagatg
    10201 ggcagattgt gttcaacctt catttaaagc atgtggagag aactgacatg cttgattcga
    10261 aaaacgtatg taagatgctg caagttagca agagctttct ttcaaggctt gtcaaggctg
    10321 aacagatcag gagttacaag atgggaagac tgagacgctt tttgctggaa gatgtactcg
    10381 attatttaag ccgtagcgaa gtgctgcaaa aagccaggga gtgcactcca agcaaaacaa
    10441 aaaaagttct caggcaagga ctggctgaca gccggtaaaa agaaaaaagg agcaaggcat
    10501 gtattttgat tattggggac ttaaaaaacc acccttcgac aatgttcctg atccatcaat
    10561 gtatgtagca tcccatacat ctgttgaaaa tacaatcgcc gaaacacttt tcgccattga
    10621 ggaaggaaat gaatgtatag ccgttattgt tggtgatgtg ggccttggga aaacaatgtc
    10681 actaaggatg gtcattgatt cccttgaacc gtcaaagtat aaaattgcgt tcataacaaa
    10741 cccggatatc ccctttattc agcttctacg agaaattatt ggacaactca cagggcgaca
    10801 gtgcgaggag aaacgaaaag ttgatctatt agagatattc aataagttac tttttcagac
    10861 tgccgatgaa ggaagaaagg ttttaatctt tattgatgaa gcaaacgcaa tgtcccctgc
    10921 gaaccttgag agtttaagac ttctcaccaa catgcaggat gacaatagaa acctttttac
    10981 cattgttctt gcaggccaaa tagaattggc aagaaggctt gagcatccca gaagggcaaa
    11041 cctttttcag aggataggga cgtactgtaa tatcgaaagg atcgagtcag aagagcttgt
    11101 caaaacctat gtggagactc gtttagccct tgcaggggca actaaaaaga tatttacaga
    11161 tgacgcaatc actgcaatct ggatttattc ggaaaatgga gtgccgagac tcatcaataa
    11221 gatttgcaaa ctttgtatga aggcaggaga aaccaacaac ctccaggaga ttgacggaaa
    11281 catagtcaag caaatctctg aacgttttca gaagctgact ggccctgcaa tgcccaagag
    11341 aaaaccaaga ttgagatcca gcgacgataa aaatattgat gaacctgggg aagcaatcct
    11401 tgaagaacaa gttcctgtgg aacctccgaa ggtagaacag aatgttgtaa tgatgaaaga
    11461 ggaagctata cagcccgaac cagcccttga tccacaacct ccattacccg aggcagcgcc
    11521 atgcgaaagc aaagaagacg gggaagaggt aagtatagga aagtatggga ttaacgtcaa
    11581 tattccacca cacatcatag agcaggccca atcattcaca gatgagcatc gttccaagct
    11641 ggcaggggtc ctggctgctc agactatgca aaaacatcca cagttgactg gtacagctga
    11701 tatggaccct gttacaatct ggagtgacat acgggagtgt atcctcctga agctcaagca
    11761 agaacctggg aaagcattcg tacagggtta aacttcttct gcttttaata ccacttctat
    11821 tccacaatac aggctagaaa tcaaaacaat tctgggtggt actgcagggg ccaatctttt
    11881 attttaagag tccagtgagt agcctttctg cacttttgtt gtgaggattg acagaaagta
    11941 cgaagttgag cctgcggatt atatcctgcc gcatttcttc actgaggtct ttttggtgtt
    12001 gcaggagctg tattctagca agattaaaat ttgtttcagc tttgtaagga ttaagcgctt
    12061 gtgcccgctt gtaaatagac tctgcacgcg acagctcatt catatcttcg tagatagcgc
    12121 caaggttgtt ccatgcagag atatcatcac catccaatac cagagcccgt ttgtaacaca
    12181 taatagcttc tttgtaccga ccttcctgtg caaaaagatt gcccaggtta aaaagagctt
    12241 ttaaatggta tgggttgatt tcaagcgttg cctcatactc tcctattgct tcgtcaaaca
    12301 tatcatgttg atcatagaga acagccaggc agaaatgggg tgctacctca tttggagcaa
    12361 cctcaatagc ttttttgtac tggataacag cgctatggta attaccttct agatcatcaa
    12421 tgtgagcgat attgatcatt gcttccacat cattaggatc aaactcaagg gttcgttcgt
    12481 acatcttttt tgcgcttccg aacctttcca tcttttcata aacataacca aggttattat
    12541 acgcttctgg ttttgtagtc tccagctctg ccactcgttg aaagcattgc aaggcttctt
    12601 catattcttc tttgcggaga tagataaaac cgaggtttac aaaagcatcg gtgtaggtgt
    12661 tgtcaatttt aatactttct ctgtatgcag caattgcgtt ctctgtttgc gacggatcgc
    12721 tgtcatacca gcagcccttg atgaaccagg ctttagctga ctttcttttg tccgttttaa
    12781 gccattgcat tgtaagcagc accaactcta aaatttaaat tgccatacaa aaaccaaatc
    12841 atcgtcaaaa aacaacagct ttatcttttt ttgttgtgtt ttgtggttat ccagggaaaa
    12901 aggtgcaata tgcaacgaca ttgaacacca tttttttact taaacaacat tggaggaacg
    12961 gactgaacac cagattgcat catcgcttcc tgatagagtt taaatttgga ctcaagttct
    13021 gttttcgtat ttaacgataa attcatattc ttcggaaatt ccttttttag atgagatact
    13081 gtcaagttaa agcaatctgc aattcctgca gcgaaatctt caagctccac ctcttcgtct
    13141 agtgtaatct ctccatgtcg gtaatcaaat tgttcggtaa aggggtcaag gaaaggatac
    13201 gtatctgatt tctcaataag agcccgttta aaagctctga gaaaaattcc ttcctgtgag
    13261 aagtcgtcaa caaatttttc tactcttgca atgatttctt gcaatgcagc taatatttgt
    13321 ttatggccat ttgaatttag tctttcatga ctgttgatgc tttctccagc aatttctttt
    13381 gtttgttcaa cttccgccaa ctcttgaatg gtttccgcgt ctattattgt tgcaggacta
    13441 tcctcatttt ttataacctg accaatcgat ttcgtatcac catgtgtttc agaagaaata
    13501 tttgttacaa ggctcctgta aacatcaaag actgctcctt gatggatcac atcttccaga
    13561 aaagttggga tagccttggg ttccgataaa gaaggacttt caagtccaga ccggagatga
    13621 agatcgataa gttccccttc ctttaagaat aaggtaccca ttggctggcc cttttttgta
    13681 aatacttcta tataccctgt atggtgttct gcgcctaact tctgaatgaa tttatccaac
    13741 ttgacaaagt cggttgttaa ccctttaaaa actatttcag cggaaaacct gctaacaatt
    13801 gcatcgacct gagcaggcga caatttatag atattcaaga tgccagcctg tttacttgcg
    13861 atgccaatga gttcgtccat ggcctcttgg ctaaaagttg ttgactgatc aggctcggat
    13921 tctataagcg caccaagagc tttaccttcc ataacaatga aggcgcctgt ttcttttgca
    13981 agtacaactt ctacaacacc agtaaatcct tctgacgaga gggttgctaa cagttctggt
    14041 atatctgtat attctgttga cagattttga tgaacaactt ctccctttgg gtatctcatg
    14101 tcatatcctc tgaattaaat tcttttcatg accttagcca ttgcctgcct ggggctttcc
    14161 attgtcccgt cttttctgca ggcgatcaat ggcagccttc acgcttgttt gcatcctttc
    14221 aatagattca gccagcactg ccacctctcc ctttgctttt acctttattg tggtttttaa
    14281 atcacccata ctgatcttgt cagcaacatc agatagataa cgaataggcc ggattataga
    14341 atgtgaatag agataaatga caacaagcaa gacaagcaga tcaatgagaa caattatata
    14401 aaagatcaca aatttattat tgtattgcga agagtacgcc ttttcaattt gtgacatttt
    14461 gtccacaata ttcttggctg gctttgagaa ttcatctatg tatgttgttg cggcaacaac
    14521 aagattagtt ccccgaacag gaatgtttac cataaacttg gggcgaactg atccatctgc
    14581 atccttccag ttgtaatacc ctgaggcagg acctgcaaga cctgcatcaa gaatcttcac
    14641 gaattcggga agctttgttg caagttgatg caagtctgtt ccaacaatct gtggattgac
    14701 atgaaaataa ttaatgcccc gattgtcatg aacagcagta tagcccgttt caccaacctt
    14761 ttgaatggca atttccttga gtctggagtt tttggaaaga acagccagag aggtattcgg
    14821 ctgagatttg acaaaaatct caatttgttt ggcaacgtcc tgggcttttt cgtaaataat
    14881 cctttccccg agtttgttca gagaggttgt agactctttc acggctgcgg tgctgaggtc
    14941 tgaaagacta ctgaacccga tatctcttat gcttttgaac tgaaacaggc caatattgat
    15001 actgaaaaat aggatgctac tgataattaa gaaagcgagc aggattggta tgaagattcc
    15061 tataataagc ctcggtaata gtcccattcc gccttttgta tcgtttgcca tgtgaaccct
    15121 cctgcttatg atttcatctt atggtcattg gcaagggcat tatgcaccac tttggtaaac
    15181 tctgcccttt ttgccatatc cggaatttca gaaacgattg cttccaatag tttgtcaacc
    15241 atttcctgag gaaatgcttc tcttgattcg ccgatttccg caataatatc atcaataata
    15301 ataggggcaa tagggcccat gagctttttt aatgcatttt ccaccatggg aaagaaacct
    15361 tcaccaacag tctttttctg caagggtttt tgctcttccg gcgcctctcc agcccgctct
    15421 aagagaccac cccgaaccat ctgaaagatt gtgttggatg ttttatacat atcccactgg
    15481 agtgctgctg caatgtccga aacacgtttt gatccgttgc atagcgctaa taccttccac
    15541 tgatctccag taatgctcct gtcttctact ccatcttgca ttgcgatacg aaacatcaca
    15601 cctggagatg gcactgattc ccggattatt ttccattctt cttctctgtt cttccaactc
    15661 gcaagcagtt gctctgtcga ctcgttcacc gttctttctt cagttgtcac ttcccagtca
    15721 aaagagactc ggccgacccg ccactccatc atggcgagga ttgcttcttc acccgtgtta
    15781 tagtttgtcc aggcgtggat gatcttgcca ccctcgatat gaatctcact gtactctctt
    15841 ccctttatcg agatcatgcc ggactttttt ccatcaagca gaggtttaac aaggtcaaac
    15901 aatcgtgtct cggacaattc accaataaag tagctaggca tacctatctc ctcatgagga
    15961 ttttatcaaa cttaaaacct ttctttgagc ggccgaggga acttcccttt cgttcagaaa
    16021 aactgttttc aggattaatg ttagtgctca aagtaataat ggaaaacctc catccacagt
    16081 gattcctcaa ggttattatc gaagaaaacg gtattaactt aactgttttt tggtataggt
    16141 ttcccctcat tcctcattga aaaaagaggg gcgtggtttg agccgaaacc tggcggatga
    16201 cgtcttaaaa tggactggat tgcaggatgt cttctgaact ttttatctag aaatattttt
    16261 acactctttc tatcccaacc gctgggatgg gaaaaacctg tatatagtga taggtctccc
    16321 acatagaagt ccctggtgtt gagctgagtc gcctcaggac tattattggg gagattaaag
    16381 actgccatat tgagaaaatc aatataaggg ctgtgtcgta ctacaaattc gagcgtttcc
    16441 tgggcttcaa cctcagtttc ccatggagta ccgaaaagga ggtaaacata tgtggcaata
    16501 ccggccttat tcagtgactt tagtgctctt gatgatcttt cgagattgtt tcccttgttc
    16561 atggcattaa gaaccgcctg gctgcccgat tccaagccca gtttaagcat cacacaacct
    16621 gaacgtctga ggtcacgaca aaaatctaaa ctcgcaagat ggttggtcag tctggcaaac
    16681 ccgtaccagg aaacccctgg tgaattggag caaagtcctg ccatgacaac agggctaatt
    16741 gcattatcta caaaatgaat gagaacaggc tgtaattctg aagataatga ttggagctct
    16801 ttcgctataa tattttttga acgcccggtg tagacgctgc cttcagcatt ctcagggcaa
    16861 aaagcacatt tactccaata acaacctgtc gatgcgttat aaggaaggat aggaccagga
    16921 gcaaggtatt caccgagagg aaagtcatga aaggaagggc aatactgctg atttttttca
    16981 gtggtacaac caagcagggc aagaagtggg tattcgccag ggcctgccac tgcatgatct
    17041 acaataccct gaaattggtt tttccacaca ttgaccctta tccatgaggt tacgaggcta
    17101 ccaccaagga caacctgaac gtcaggcatt gtttccttta taaacccgat tatcgcaaag
    17161 gtgcataacg cctggctcag gtagttaagg gaaagaccca ccaaatctgt tccagttttt
    17221 tccagggcag ccctcaatga atctctgaaa taggggtaaa aaaagttttg tttgtattct
    17281 tcagcagcct tgaggagatc agcactcctc agaggggaaa gatcaatgtg ttggtagtca
    17341 gaaaaagata agcgtttaat ctgagaacct ccatgtacct gaaaaatacg atgcaaatct
    17401 gtcactatgc tcctgtaccg ttctgtggaa ccataaccct tccatgacct taatatgctg
    17461 atattctgat ggaggttgcg ctgtgctctt aatgtccatg tgtctgacgg tgtttttgct
    17521 tgaccggata gaacggcaag aagacattca agatttaaat caagaacact gtggtttatc
    17581 ccatgggctg tcaatgcacc agaaagttta gagatgccgg ctggtggttc gcaaggtttt
    17641 acaactggag gatgaatgag aagtatgcca ttatgcctga tcatgccgtt tattataaag
    17701 cattttttat tagaacttca cagaaagata ttacactttc catccaactg tttgaccaat
    17761 gggaaacctt gtgatacctt tatacactat ttaaatcata aaaggcattt atgtctgaca
    17821 atgtaacccc tctgcacaag ctacgcatat ttaaagaaca aataggaatt gatgcagcag
    17881 atttggcaga gctagcccca tttcgtcact tcttcactga aagaaaagag gagtttggtg
    17941 attattttta caatgtcttt tgggggatac ctgccacaag aacgatactt gaaggtgaaa
    18001 aaacccgggg cgtcttaagg aagacctggt cgttatggtt tgagaccttc ttttcggctg
    18061 attttgatga tacatttctc agctggcttt ggaagattgg tgtgagacat gtagaagtaa
    18121 atctcgatca aaggttttca aatctcggct ttgcagtcat ccgtcagttc tgccatggaa
    18181 ttgttttcaa agaattgccc ctccaacaaa ggggtcgggt tctttcaata atagataagc
    18241 ttcttgatct ttgtgtcctt gttgagacca gcgcatatat tgaaaataca atcagttgca
    18301 atattgaggt aatgcgtgaa atggcagata gggtgagaaa cccagccatg atcatcgggt
    18361 ggaatataaa aagacttcac gataaagctg atgcagatag taaagagtac agggtataca
    18421 agatgctcat ggaagaaaac gagaggcttg aaggaatggt gagagatata aaggtctaca
    18481 tggatctttt cgagactgaa ccggagatcg acactgtaaa ttttgaagaa attattaacg
    18541 aggtgctcct taagctcaaa gaagaaggaa cag
//
LOCUS       DAOOMC010000052        18502 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdus sp. isolate AMR_MDS_2685
            NODE_3083_length_18502_cov_6.334905, whole genome shotgun sequence.
ACCESSION   DAOOMC010000052 DAOOMC010000000
VERSION     DAOOMC010000052.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864760
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdus sp. (wastewater metagenome)
  ORGANISM  Syntrophorhabdus sp.
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae; Syntrophorhabdus.
REFERENCE   1  (bases 1 to 18502)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18502)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,729
            Genes (coding)                    :: 2,708
            CDSs (with protein)               :: 2,708
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 7 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18502
                     /organism="Syntrophorhabdus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3083_length_18502_cov_6.334905"
                     /isolate="AMR_MDS_2685"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2699756"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            496..1440
                     /locus_tag="PK125_06835"
     CDS             496..1440
                     /locus_tag="PK125_06835"
                     /inference="COORDINATES: protein motif:HMM:NF020130.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HPB37858.1"
                     /translation="MYITTASHYLPNTTVFNEYYTRLMGLSDEWIFKRSGIRSRSKTN
                     IGENTNTMAIEAVKAAIDRLAYPIRDVDLIVGATYTPYDTVGTLAHAVQHFFDIAGAR
                     VISISSACSSFLNAVEIVEGYFATNKSKRALVVSSEHNSAYSDDMDEQSGHLWGDGAG
                     AVFISKERISDDDIKILDLNTTGLGHIGKGVEGVYLRPNNGGLKMPFGKDIFVNATKY
                     MVSEVKDILKKNNLALDKIVHLIPHQANSRIIAQVAEALELRNGELVTNIEETGNTGC
                     ASTMIGLSQNWQRFKKEELIVITVFGGGYSSGAMLLKK"
     gene            complement(1494..3236)
                     /locus_tag="PK125_06840"
     CDS             complement(1494..3236)
                     /locus_tag="PK125_06840"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HPB37859.1"
                     /translation="MNILIIGGGISGISAAKVALAEKHDVTILESTPEPGGLMARIAN
                     CRVGFKTFFDEIRDNEKLSVISDTRISKVDKTEKVWTITLEDGRELTADRIIIATGLT
                     PYDPVEYKGKRVLTSLEFDSVIDQRQGELPDDFNKIGFFLCVGSRSKDYPLCSSVCCS
                     YTLREVKWTLQRAKPEITVFYNDLRFFGQEFYLEKAYRDAGVRFIRANSRYFEEDEKG
                     VTVRYFSGDQLKEERFNYVVLAIGLRPNPQLTELGKLFGFSLNEYGFVKEKTPLQTDV
                     EGICVSGGALEPMNIKDSILTGFGAGLLTVRGHEIGMAQEKQDDRLYHEDEPEFSFPD
                     SNITTYLFYLGTEDYTSAMFYEYVSSKFIEKARELKKEGKAVYVITRNMVVPSYAELD
                     YEEARREGIVFVHLEEDEKIVFETDCVRIQGGGRELIFNADKVIHFDEYASLMQNREF
                     LSLYRSEPQIRWSPTKWGRKKYHVGFIRHPREKRWEKRELLGALGEMILDHEEDRVLP
                     EFNEERCSGCGSCKEACPAGAIEILIREKQIAIFGPVTSTGVPIAHVKEDTCIGCGLC
                     ASTCPSDVISHSKL"
     gene            complement(3233..3913)
                     /locus_tag="PK125_06845"
     CDS             complement(3233..3913)
                     /locus_tag="PK125_06845"
                     /inference="COORDINATES: protein motif:HMM:NF014774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heterodisulfide reductase-related iron-sulfur
                     binding cluster"
                     /protein_id="HPB37860.1"
                     /translation="MEYGYYPGCSLTGSAKKLNRGVREICKKLGHTLSEIPDWNCCGA
                     VEYGDRNELVRISGENLKKAEGIHREIVVPCPACYKNLKDADSAGSFNILSPLELLSP
                     ENLSQMKQLKDLRGQVFFPYYGCLLMRPEGSSIKNKNIMEDAITVFGGEIDGEKMKDR
                     CCGGNQLFLNKWATEKLSSLILSKSRGTVVVFCPFCHMAMKTFGVGRKVIYLTDLLLY
                     VMGEKNSL"
     gene            complement(3913..4428)
                     /locus_tag="PK125_06850"
     CDS             complement(3913..4428)
                     /locus_tag="PK125_06850"
                     /inference="COORDINATES: protein motif:HMM:NF024924.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HPB37861.1"
                     /translation="MNKKDHEMVFRLTGYNREDFSVCLGCKICASVCTVNDLELNVNP
                     QELLLKLFTGEEIKDDNPLVHYCTNCYRCTVACPWEIRIPDVVRAVKESLGIDSLFEK
                     AFKKSLNIWGRVYEPFVVVMAAPDLLKGGYIKHMPRWMEYAVFHLPHKVKRLGSSKVS
                     KENNPSEEGRR"
     gene            complement(4484..5494)
                     /locus_tag="PK125_06855"
     CDS             complement(4484..5494)
                     /locus_tag="PK125_06855"
                     /inference="COORDINATES: protein motif:HMM:NF024581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HPB37862.1"
                     /translation="MKGKKLKTGEIENALNRFLKEEQHLVLGFKKDESGVSHHIFKGS
                     LDHYSLLNPVFGANAALYLRRLFSKGSQVMLMLRPCEIRACVELNKLTQIEREDIIAV
                     SIDCPGTVSSKEKKEEVPAELHELNDYFKNKDSMRWACSVCGEKRAVVGDAGIRIDEK
                     GNYWAIPYTEKGEVLLSLIDGEPEEVPAELLAAKEQKKDKFQVDMEIFNKDFEKCIMC
                     MNCRDMCPVCYCIDCVFNGDEYLPKGDALLNKVFRIGSVKMPRGKDLFHFIRMYHVSQ
                     TCVGCGACEEACPQGIPLTKYFKGVSERLQDIFSYMSGRSFDEPIPYVTFLEDELKDA
                     TD"
     gene            complement(5475..5894)
                     /locus_tag="PK125_06860"
     CDS             complement(5475..5894)
                     /locus_tag="PK125_06860"
                     /inference="COORDINATES: protein motif:HMM:NF014692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase iron-sulfur subunit"
                     /protein_id="HPB37863.1"
                     /translation="MTDEAKEKNPEIVGFACSYCTFKASEMAGSLRMKYPEGVKLIQV
                     PCSSRVDPAFVIKAVFEGADGVFVAGCHPGDCHFIKGNYYTRRKIVALKEMFDAFSMK
                     EKLRLFWVSAAEARRFIDKVTTFYNDIKEKKNEREKA"
     gene            complement(5887..6327)
                     /locus_tag="PK125_06865"
     CDS             complement(5887..6327)
                     /locus_tag="PK125_06865"
                     /inference="COORDINATES: protein motif:HMM:TIGR02436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HPB37864.1"
                     /translation="MERLAKRVQDLVIYKLAFETAMEIFEITKSFPSEEKYSLTDQIR
                     RSSRSVCTNLAEGWRKRKYKAVFVNKLLDAAQEAAETQTWLEFSDRCNYFAFVVFEEL
                     YERYEHIFAMLKTWKRNLENSVNVQRPSVANVLRPTVNRGRYND"
     gene            complement(6375..8048)
                     /locus_tag="PK125_06870"
     CDS             complement(6375..8048)
                     /locus_tag="PK125_06870"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoB--CoM heterodisulfide reductase iron-sulfur
                     subunit A family protein"
                     /protein_id="HPB37865.1"
                     /translation="MKTGIFICHCGHNIKHTVDVKKLSEYFKKFPNVTVSQDYVFVCS
                     EPGQDMIKEAIEKEGLDRVIIASCTPSLHGEMFKDVLKKADLNPFLLRRVSIREHCSW
                     VGDDIEANTEKAKRYILAGLYSSAHHVPLEEKIVEVEKSALIIGGGVSGLSAALFLSK
                     MGMQVYLVEKEAELGGHVRALKNVWPSGRDGKSIVDEMVAELQSRNNVEIFTSTTIGG
                     FEGFFGNYEVKLDTPEGQRKVIAGGAIIAIGFSPFDPSIKPELGYGYDKRVITTLEFE
                     QNVDQLQLPENPRVAILHCVGSRDDQLGKPYCSRVCCVNALRVGDALKSRYKDSYVES
                     FYMDVRAHPKGGEEFFEDTQEKGVLFTRANVAEITPTPTGVVVRGEDTLFGEIFERRF
                     DLVVLSIGMSPPEDSKLIANLLKITLDKDQFFLEAHIKLRPFDTAVKGIFIAGSCSGP
                     KDVEESINHGRASAVKLFGFLNLGYAYVDPFISWVDPKRCSGCRMCEQACVAKAIKYD
                     EEKRIVRVEEAACMGCGLCNATCPSSAIILKGYIDRMIDDEISAFMEAI"
     gene            complement(8143..>10843)
                     /locus_tag="PK125_06875"
     CDS             complement(8143..>10843)
                     /locus_tag="PK125_06875"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HPB37866.1"
                     /translation="TVAEKNVLVIGGGIGGITAALELASCGVKVTMLEEGPSIGGRMI
                     QLDKTFPTLDCATCVLSPKMVEVSLNQNIEILSWAKPLAVKKKGTGFQVTIFKKTRYI
                     DTKKCTACGSCSTGCPVVMKSEFNVGTGPRKAAYIPFPQAIPNKATIDKRENRPCKAA
                     CVDACPINTNVLGYLKHISEGRFQDAYMLIRATNPFPSVCGRVCYAPCEGVCNRGQLD
                     EPLAIRDLKRFAVDRFDVHSLEVPQITKTEKKVAVIGAGPAGLTCAHDLALEGHEVTV
                     FEALPEPGGMLRYAIPEYRLPREEVRKEIGYIEKLGVTIQCGTEIGKDITLDTIRNDY
                     DAVFIGTGTPKGLPLEVEGENLPGVIDGIRFLRSVNSGEAVKTGQRVAVIGGGNTAVD
                     CARTVKRLGSESVKLIYRRTRDEMPAAREEVEALLHEGIEIDFLTTPIRFHAENGELA
                     KMECIRMELGEPDGSGRRRPVPVKNSEFMVPVDSVITALGQAAQTAFTQVVGVLLAKN
                     GTIDINPATGATNVEGIFAGGDVYTGPAYVVDAIAAGQRAAKSINRYLKGEPVVVTKE
                     RKEPEKLTDAEIADLTAKYPRTERIHMPEATVEERIKNFEEVAMGYSTEEAMAEAARC
                     LAGQIEGCIQCGECERLCEVGAIDHTMKDEIVELDFDSIILAPGFDLYDPTEKKEYGY
                     GSLDGVMTGMEFERLSSVTGPTGGDIVLNGKVPKRFFFIQCVGSRDRQSGARYCSRVC
                     CMYTAKHASIIRDRIKDIQIYVSYIDVRAYGKGYEEFYKTTQENGTFYIRGIPGEVTK
                     SENGLLVRVEDMFSGELLEIEVDLVILATGVRPRKGIEELCDIMSLERDEYGFIRADS
                     INPSKTNVEGIFVCGMGSGPKDIPDTVASGGEAAASCMEYITE"
     assembly_gap    10844..10943
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(11185..12123)
                     /locus_tag="PK125_06880"
     CDS             complement(11185..12123)
                     /locus_tag="PK125_06880"
                     /inference="COORDINATES: protein motif:HMM:NF013200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HPB37867.1"
                     /translation="MSYALVVSEVRRGIFEERNLDSIGLCTHLGKDAILIIPEGEYGI
                     KEQLVNSIVKVKVEETVFLNPLNMVNILEKVMADRGKPDMIVFTNSSSGTELASYAAG
                     YFDLPIITDVNSCDKDQGIFYKSYYSDKIFGEFRPIGNGTAIVTVRSGSFKDFAADKD
                     VAPSVGTMEDVAVSSARTFMEFVEEEKSDVDITKAEFLLSLGRGVGNKDEVSDYEELA
                     GMLKAVIAGSRPVIDKLWLPKARQVGTSGKTVKPKVYLAMGVSGAFQHVAGMKDSDCI
                     IAVNKDAEAPIFQYAHYGIIADMHKVRDKLKDILKG"
     gene            complement(12120..12899)
                     /locus_tag="PK125_06885"
     CDS             complement(12120..12899)
                     /locus_tag="PK125_06885"
                     /inference="COORDINATES: protein motif:HMM:NF013200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HPB37868.1"
                     /translation="MNIIVFTKRVPATQEEELRIIGEGSAVDLSKVPFKVNDWDNYSV
                     EEAVRIVEKSGGAVTAVSVGDAESDEVLRRAIAMGAKDGFLVESSDIVHDPNARATLI
                     QNFLKKENVPFDAIFTGVQSEDDQFASLGGILAAKMRLPFVSMVIGIDSFEKDHAVVR
                     RELEGGLQERVKVMLPCVMSIQTGINEPRYVSIMGIRKASKVERKIFKSADYQEKVTA
                     KIEVARWIYPPKKAGATILSGELESACKELIGILKEKGVYQ"
     gene            complement(12901..13995)
                     /locus_tag="PK125_06890"
     CDS             complement(12901..13995)
                     /locus_tag="PK125_06890"
                     /inference="COORDINATES: protein motif:HMM:NF014774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HPB37869.1"
                     /translation="MIAQTQTRVLTCIQCGKCTGSCPESGRTPFNVRMLMRKKQFQNA
                     IEESIPWYCTSCGSCTIRCPRDVKPSEVIIEMRAALVENGSIPVTIQKALENTFVQKN
                     PWGRSRAKRGAWFEKMDIEIPHVSETETKRLLFACCIQAYDPRCMVIPVNVATILKKA
                     GIEFGVLGEEEACCGNEIRRIGESGLFEELQEENRATFEEYGVKEIIALSPHCMNALK
                     KEYGDLGIKISHYTEVLAPMFTYGTVVPKGSYNKKVIYHDPCFLGKQNGIFDEPRDIL
                     KSVNGLELLEFSRSRDNSLCCEGGGGRMFFEVEATYQRNAEVRVQDAVNRGAEEIVTS
                     CPFCVMTLEDPATEKGIKVKELSEILMEVL"
     gene            complement(14400..15545)
                     /locus_tag="PK125_06895"
     CDS             complement(14400..15545)
                     /locus_tag="PK125_06895"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HPB37870.1"
                     /translation="MVELTAEQRDIKMAAQEFAEKEFRDIARELEAKEEFDDRIWKKT
                     AENGFLAVFVPEEYGGQGLGHFEQCLILEEFARVDLGITHQIDSTFFGAQFILFNGTE
                     EQKKKYLPPVCEGKWRIGMASTEPEAGSDVASIATTATKDGDYYVLRGNKVFITNCTT
                     SDFLVVLCVTTPEAKRKHERLGAIVVDTKSPGYEATTWHGKLSLRCSTTGEVAFNDMR
                     VPRENLLGEEGKGFYYLMDFFNHTRLGVAAFGVGTAQGALDKAVAHVKKRKQFGATLA
                     ALGVVQTKIAEMGTMVETARSLLYRAALKADRGEIDPALVAMSKWYASEVAVKVADEA
                     IQLHGGSGILVEYDVEHYWRDAKVLEIFEGTKEIEKLLIGRRLLGLH"
     gene            complement(15883..17427)
                     /locus_tag="PK125_06900"
     CDS             complement(15883..17427)
                     /locus_tag="PK125_06900"
                     /EC_number="6.2.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF004837.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain-fatty-acid--CoA ligase"
                     /protein_id="HPB37871.1"
                     /translation="MTVGEMLTRNANKFPTKKAVVSEETSIDFKTLYERVNRLANGLL
                     KKGLKKGDRIGVLIHNCHQFIEIYYGAAKTGGIFCPYNNHLKQWELKDIIDYSTPRFL
                     FVDKDFVETINNLKSELKSVESFICLQKTDGAYWEDYEGLIAQSDAAEPRIRILEDDV
                     QSIIFTAGTTGRPKGAMRTHRHLMSDAFASAIDLQVGYDEKVLITFPMYHVACEDNIV
                     RHSLLSNTIYTKKEGGFNPPEVLEYISKERISRIQLVPTMIHSLIQVPDVSKYDLSSL
                     KLILYAGSSMPVELLKKALTIFPCGFAQLYGQTESGPFTTVLRPEDHIQDGTEKSVAR
                     LASSGKPALNYEVRIVDDNDVDVPMGEVGELIVRSEAMMIGYWQMPEETEKKLKNGWL
                     HTGDLAKFDEDGYVYIVERKGDMIISGGVNIYPREIEEVLYRHPAVSEASVIGLPDEH
                     WGEIVKAVIVLKPGAKATEDEIKDFCGKNLAGFKKPKSVDFWKELPKSPQGKILKKDI
                     RKQYRK"
     gene            complement(17575..>18502)
                     /locus_tag="PK125_06905"
     CDS             complement(17575..>18502)
                     /locus_tag="PK125_06905"
                     /inference="COORDINATES: protein motif:HMM:NF018513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HPB37872.1"
                     /translation="SVAVPEMTKFGYAKKLSTGIVATVGTLGVLIPPSVVLIVFGIVT
                     QLSIGKLFLAGIIPGLLIAFFFVVVIFGWCKINPGLGPKSDRFTWGERVKTLPDVIWP
                     IVIFFIIIIGLMKGIFTPTEAGSIGAFTVLLLCLFKRNLNFMGYVKSVQEALRTACMV
                     LLLVATSNILGHFIAVTNIPNHLGAWVLGIQMPAIFVLILVFFVYLIGGSFIDDLAFM
                     ILATPIFYPIMEKLGYDPLLIGILVALTVCIGSVIPPVAICVFVVKNITKVPMGTIYA
                     GVYPFLISLVVVVVLCIAFPQLVLYLPNTLMP"
BASE COUNT         4857 a         4703 c         3522 g         5320 t
ORIGIN      
        1 ggagatacgg cagtgtacgt tgccaaccag gctctgcagc ttcatggcgg ttacgggtat
       61 attgccgagt acgacatcca gagattttac cgggatgcaa agatcgttga gatttatgag
      121 gggaccaaag aggtggaaaa aactattata ggttcaggct tgctgaaata gtttcgtaat
      181 agggatatac acaggtataa aacaaaatag atacccgtca ggtaggtcat caagctcacc
      241 tttaccgaat tgtcttgaca cgcacgtatc tccccggtat acttgtttcg ccttgaaatg
      301 gctttcaaag actcctctgc taatcgtttg cctattaatg tataattcga acctggcaaa
      361 cacggtctga ttctatccgc attcttgcaa atcagcaaaa gctcaaggtt tttttgaaaa
      421 ttgcaagcag cattatatga gaattgaacc aatgagaaac aatgaaaaaa gtatctgttt
      481 acgatgagtc aacccatgta tattaccacg gcctcacact atcttccgaa caccactgtt
      541 tttaatgagt attacacgag gctcatggga ctttctgacg aatggatttt taagcgttct
      601 ggaatccggt cccggtcaaa aacaaacatt ggcgaaaaca caaacaccat ggcgatagaa
      661 gcagtgaaag ctgcaatcga tcggctggcc tatccaatca gggatgtcga ccttatagta
      721 ggggcaacct acacccccta tgatacagtg ggaacgcttg cccatgctgt gcaacatttt
      781 tttgatatcg ccggagcaag ggtcatctcc atttcctcag cgtgttcctc tttcctcaat
      841 gccgtagaga ttgttgaagg atacttcgct acaaacaaat caaagagggc ccttgttgtt
      901 tcttcggaac ataatagcgc atacagcgat gatatggatg agcaatcagg tcatttatgg
      961 ggtgacggtg ctggagctgt atttatctca aaagaaagga tatctgatga tgatataaag
     1021 attcttgacc tgaacacaac agggctaggt cacatcggca agggtgtcga gggggtttat
     1081 ttaagaccaa acaatggggg cctaaagatg ccttttggca aagatatttt tgtcaatgct
     1141 accaagtata tggtttcaga ggttaaagat atcctgaaaa aaaacaattt ggctcttgat
     1201 aagatcgtcc acctcatccc ccatcaggcc aactccagga tcatagcgca ggtcgctgag
     1261 gcccttgagc taaggaatgg agagctggtc acgaatatcg aagaaacagg caacaccggc
     1321 tgcgcaagta caatgattgg actttcccag aactggcaac gtttcaagaa ggaagaactg
     1381 attgtaatca cagtatttgg tggagggtac tcaagtggcg ccatgcttct gaagaaataa
     1441 taggggaatt ctttctttat tcaataaaaa ctgggtgtat ctgcgtacgg ctatcagagt
     1501 tttgagtggc tgatcacatc agatggacac gttgatgcgc aaagaccaca gccaatgcag
     1561 gtgtcctcct ttacatgggc aatgggaacg cctgttgatg ttaccggacc aaaaatggct
     1621 atctgtttct cgcggatcaa aatctctatg gcacctgcag ggcacgcctc tttgcatgag
     1681 ccacacccac tgcagcgttc ctcattgaac tctgggagta ccctgtcctc ttcatgatca
     1741 agaatcatct cacccaaagc tccaagaagc tctctctttt cccatctttt ttctctggga
     1801 tgtctgatga atcctacatg gtacttcttc cttccccatt tggtaggtga ccaccttatt
     1861 tggggttcag accgataaag ggagagaaac tccctgtttt gcataagcga tgcatactcg
     1921 tcgaagtgga ttactttgtc tgcattaaaa ataagctcac gcccacctcc ttgaatgcgg
     1981 acacaatctg tttcaaatac aattttctca tcctcttcaa ggtggacaaa tacaattcct
     2041 tcccgccttg cctcttcata gtcaagctcg gcataggaag gaacaaccat gtttctcgta
     2101 atcacataaa ctgctttccc ttcctttttc aattctcttg ctttctcaat gaatttcgat
     2161 gagacatatt cataaaacat ggctgacgtg tagtcttctg tgcccaggta aaacagatag
     2221 gtagttatat tactatcagg aaaggaaaac tcaggttcat cttcatggta aagccggtca
     2281 tcctgttttt cctgagccat ccctatttcg tgccccctga ctgtcagcaa tcctgcacca
     2341 aaaccggtga gaatggaatc cttgatattc attggctcaa gcgcaccgcc agacacacag
     2401 ataccctcca catcagtctg aagaggagtt ttttccttaa cgaacccgta ttcattcagt
     2461 gagaaaccga aaagtttgcc cagctcagtg agttgcgggt ttgggcgaag cccgatggca
     2521 agtactacat agttaaaccg ttcttccttc agttgatctc ccgagaaata ccgcactgta
     2581 acgccctttt catcctcctc aaaataccgg gagttagctc ttataaatct cacacctgca
     2641 tcccgatagg ctttctccag gtaaaattcc tggccaaaaa acctcagatc attgtaaaag
     2701 acagtgatct ctggttttgc cctctggagc gtccatttca cttcccgcaa ggtataggaa
     2761 caacagactg aagaacaaag agggtaatcc tttgaacgag atccgacaca gaggaagaag
     2821 ccaatcttgt taaaatcatc tggaagctct ccttgccgct ggtcgatcac agagtcaaac
     2881 tcaaggctcg tgagaaccct tttgcccttg tactctacag ggtcataggg agtcaggccg
     2941 gttgcaataa ttatcctgtc cgcagtcagc tcccgcccat cttcaagggt tatagtccaa
     3001 accttttctg ttttgtcgac ttttgaaatc ctggtatcac tgataactga aagcttttca
     3061 ttgtcacgaa tttcatcaaa aaaggtttta aaccccaccc tgcagtttgc aatacgagcc
     3121 atgagcccac ctggctcggg ggtggattcc agtattgtca catcatgttt ttctgccagg
     3181 gcaacctttg cagctgatat accgcttata ccaccaccta ttatcaatat attcataaag
     3241 aatttttctc tcccataacg taaagtagaa gatcagtcag ataaataacc tttcttccta
     3301 cgccaaaggt tttcattgcc atgtggcaaa aggggcagaa aacaacaacc gtccctctgg
     3361 acttgcttag tatgaggctt gaaagttttt cggtagccca tttattgagg aaaagctgat
     3421 tgcctccgca acaacggtcc ttcatcttct ccccgtcaat ctcaccgcca aagacggtta
     3481 tggcgtcttc catgatgttc ttgttcttaa ttgagcttcc ttcgggcctc ataagcaggc
     3541 atccgtaata cgggaaaaag acctggcccc ggagatcttt aagttgcttc atctgtgaca
     3601 ggttttcagg gctcaaaagt tccagagggc taagtatgtt gaaagacccg gcagaatcag
     3661 cgtctttcag gtttttataa caggccggac agggcacaac gatttccctg tgaataccct
     3721 cggccttttt gaggttctct cctgaaatac ggacgagttc atttctatcc ccgtactcta
     3781 cagcgccaca acagttccaa tcaggtatct cggatagggt gtgaccaagc tttttacaga
     3841 tttcccgtac gccacgattc agtttttttg ccgaaccagt aagggaacat ccgggataat
     3901 aaccgtattc cattatcgcc taccttcttc agaagggttg ttctcctttg aaactttgga
     3961 agaaccaagc cttttaactt tatggggaag gtggaagact gcatattcca tccaccgtgg
     4021 catgtgcttt atataaccac cttttaagag atcgggtgca gccataacca ccacaaacgg
     4081 ctcgtacacc ctgccccaaa tgttaagtga cttcttgaat gccttttcaa agagggaatc
     4141 tataccaagc gattccttta cagcccgaac cacatctggt attcgaattt cccaaggaca
     4201 ggcaacggtg caccgataac aatttgtaca gtaatgtacg aggggattat cgtcttttat
     4261 ttcttccccg gtaaaaagtt tcaagaggag ttcctgtggg ttgacattca gctcgaggtc
     4321 gttgaccgtg cacacagatg cacagatttt gcagcccagg cacacagaaa aatcttcccg
     4381 attgtaaccg gtaagcctga aaaccatttc atgatctttc ttattcatcg ttctctactt
     4441 cgcttttatt tggttcttcc ttctcccatc ttctctcttt ttatcaatcc gtagcatcct
     4501 taagttcgtc ctcaagaaag gtaacatagg ggataggctc atcgaagctt cttcctgaca
     4561 tgtaagagaa tatatcctgg agcctctcag acacaccttt aaaatatttt gtcaggggta
     4621 tcccctgtgg gcaagcctct tcgcacgcac cacaaccaac acaagtctgt gacacgtggt
     4681 acatccttat aaagtgaaaa agatcctttc ccctcggcat tttcactgag ccgattctaa
     4741 aaaccttgtt gagaagggca tcacccttcg gaaggtactc atctccgtta aaaacacagt
     4801 caatacaata gcagacaggg cacatatctc tgcagttcat acacataatg catttttcaa
     4861 aatctttatt gaaaatttcc atgtctacct ggaacttatc ctttttttgc tccttagctg
     4921 ccaataactc agcaggaacc tcctcgggtt caccgtcaat aagggaaaga agcacctccc
     4981 ctttttccgt gtaagggatt gcccaataat tccccttttc atcaattctg atacccgcgt
     5041 cgcccacaac cgctcttttt tcgccacata cgctgcatgc ccatcgcata gagtctttgt
     5101 tcttaaaata atcgttcagc tcatggagtt ctgcaggcac ctcttctttc ttttccttcg
     5161 aagagacagt cccgggacaa tcgatagaca ctgcaatgat gtcttcccgc tctatctgcg
     5221 tcagtttgtt caattctaca caagccctta tctcgcaggg tctcaacata agcattacct
     5281 gtgacccctt ggaaaacagt ctgcgcaagt acaacgctgc attggcacca aaaacagggt
     5341 tcaggagaga ataatgatcg aggcttccct tgaatatatg gtgactgaca ccgctttcat
     5401 ccttcttaaa tccaaggacg agatgctgtt cctccttgag aaaccggtta agagcatttt
     5461 caatttcgcc ggttttaagc tttttccctt tcatttttct tttcctttat atcattatag
     5521 aacgtggtga ccttatcgat aaagcgtcgt gcctcagcag cactcaccca gaaaagacga
     5581 agtttttctt tcatagagaa ggcatcgaac atctctttca atgcaactat cttacgcctg
     5641 gtgtaataat tgcccttgat aaagtgacag tcaccaggat ggcatccggc aacaaacacg
     5701 ccatccgccc cttcaaagac agccttaata acaaatgctg ggtcaaccct actcgaacac
     5761 ggaacctgaa tgagcttgac cccttccgga tatttcattc tcaggctacc tgccatttca
     5821 gatgctttga atgtacagta agagcatgca aaaccgacta tttccggatt tttctctttt
     5881 gcctcgtcag tcattgtacc tccctctatt aaccgtaggg cgtaggacgt tcgctacgct
     5941 tgggcgttgg acgttcacag aattctccag atttcttttc catgttttta gcattgcaaa
     6001 aatgtgttca tacctttcgt ataattcttc aaaaaccaca aatgcaaaat aattacatcg
     6061 atcagaaaat tccaaccacg tttgtgtttc agcagcttct tgcgctgcgt cgagaagctt
     6121 attgacaaaa acagctttat attttcgttt tctccatcct tctgccaaat tcgtacaaac
     6181 tgatcttgat gatcgtctga tctggtcggt gagagaatat ttctcctcag atggaaatga
     6241 tttcgtgatt tcaaatatct ccatagcagt ttcaaaggcc aatttgtaaa tcactaaatc
     6301 ctgtacccgc ttagctaatc tttccatatt ctaacaatct tacgttccgt cccccatcct
     6361 acgtcacacg gtaatcagat cgcctccatg aatgcgctaa tctcgtcatc tatcattcta
     6421 tcgatatacc ctttgagaat tattgccgac gacgggcagg ttgcgttaca gagaccacac
     6481 cccatgcacg cagcttcctc aacacggaca atcctctttt cctcgtcata ttttattgcc
     6541 tttgcaacgc acgcctgctc gcacattctg catccgctgc atctttttgg atcaacccat
     6601 gaaataaagg gatccacata agcgtaacca agatttagaa acccaaaaag cttgactgcc
     6661 gatgcccttc catggttgat cgattcttca acatccttgg ggccggaaca cgaccctgca
     6721 ataaaaatcc cctttacagc cgtgtcgaat ggtcggagtt taatatgcgc ctcaagaaaa
     6781 aactgatcct tgtcgagagt aattttcaag aggttcgcga ttaatttact atcttctggt
     6841 ggagacatcc cgattgataa aacaacaaga tcgaatcgtc gctcaaagat ttcaccaaaa
     6901 agtgtgtcct ctccacggac cacaacaccc gtgggtgtcg gagttatttc tgccacattc
     6961 gctcttgtaa aaagaacacc cttttcctga gtatcctcga agaactcttc cccacctttt
     7021 ggatgggccc gtacatccat gtaaaaagat tcaacgtagg aatccttgta tctggacttg
     7081 agagcatccc ccacccgtaa ggcgttgaca cagcacactc ttgagcaata aggttttccc
     7141 agctggtcat cccgtgatcc cacgcaatgg agtattgcaa cacggggatt ttcaggaagt
     7201 tgcaactgat cgacattctg ctcaaattca agggttgtga tgacccgttt gtcatatccg
     7261 taacctagtt caggtttaat gcttggatca aagggtgaga aaccgatagc aattatggcg
     7321 ccacctgcta ttaccttccg ctggccttct ggagtatcca gcttcacttc atagtttcca
     7381 aaaaaaccct caaaacctcc gatagttgtc gaggtgaata tttctacatt attacgactt
     7441 tgcaattctg cgaccatttc atcaacaata ctttttccat ctcttccaga gggccatacg
     7501 tttttgaggg ctctcacatg accgccaagt tcggcttctt tttcaactag ataaacctgc
     7561 atccccatct tcgacagaaa cagcgcggcg ctcaaccctg acactcctcc acctataata
     7621 agtgcggatt tctccacctc tacaatcttc tcttcaaggg gaacgtgatg agcggacgaa
     7681 tagagccccg caagtatata tctctttgct ttttcagtgt ttgcctctat atcatcacct
     7741 acccaggagc agtgttccct gatactaacc ctccttaaaa gaaatggatt caggtctgcc
     7801 ttttttaaga catctttaaa catttctcca tgaagagagg gggtgcatga agcgataata
     7861 acacgatcaa ggccttcctt ttcaattgcc tcttttatca tatcctgacc aggttccgag
     7921 cagacaaaca cgtaatcttg cgaaacagtt acattcggaa atttcttaaa atactcgctc
     7981 agctttttga catcaactgt atgcttaata ttgtggccgc aatggcagat aaagattccg
     8041 gttttcatat tcaaacttcc gtgacgcgtg acgcgtgacg cgtaatcagt tgatttttcc
     8101 tatcgcccat agcctatagc ccaccttatt acttttcact tttcactccg ttatgtactc
     8161 catacagctt gccgctgctt cacctcctga agctactgta tcgggaatat ccttaggtcc
     8221 tgagcccatg ccacatacaa agattccttc cacattcgtc tttgagggat ttattgagtc
     8281 tgcccgaata aaaccatatt catcgcgctc caacgacata atgtcacaga gttcttcgat
     8341 tcctttgcgg ggccttacac ctgttgcaag tattacaagg tctacttcaa tctcaagaag
     8401 ttctccactg aacatatctt ctacccgtac caacaggcca ttctccgact ttgtcacctc
     8461 tccgggaata cctcttatat aaaatgtacc gttctcctgg gtggtcttgt agaactcttc
     8521 gtatcccttg ccataagccc tcacatctat gtaagacaca taaatctgta tatcttttat
     8581 cctgtccctt atgatacttg cgtgttttgc tgtatacatg cagcatactc tggaacaata
     8641 ccgggccccg ctttgtctgt ctcttgagcc gacgcactgt atgaaaaaaa accgttttgg
     8701 gacttttcca tttaacacga tgtcgccccc cgtgggacca gtaacagatg ataaccgttc
     8761 aaactccatt cccgtcataa caccgtccag tgaaccatat ccatattctt ttttttctgt
     8821 gggatcatat aggtcaaagc caggagcaag tattatgctg tcaaagtcca actctacaat
     8881 ttcatccttc atagtatgat cgatagctcc aacttcacac aatctctcac attccccaca
     8941 ctggatacat ccttctatct ggccggcaag gcagcgagcg gcttcagcca tggcttcttc
     9001 agtgctatat cccatggcca cttcttcgaa attctttatt ctctcttcta ccgtggcctc
     9061 aggcatatga atgcgctctg ttctagggta tttcgctgtt agatcagcaa tctcagcatc
     9121 ggtcaatttc tccggctctt tcctttcctt tgttacaacc accggttccc ctttcaggta
     9181 gcggtttatc gatttcgctg ccctctggcc cgcagcaata gcatcaacaa cataggcagg
     9241 tcctgtataa acatcacccc ctgcaaatat tccttccaca tttgtagcac cggtagcggg
     9301 attaatatcg atagttccgt tttttgcgag caaaaccccg accacctgag taaaagctgt
     9361 ctgcgcagcc tgaccaaggg cggtgataac cgaatcgact ggcaccataa actcagaatt
     9421 cttaaccgga acaggccttc ttctgccgct tccatcaggc tcaccaagct ccatacggat
     9481 acactccatt tttgcaagtt ctccattttc tgcgtgaaag cgaatagggg ttgtgagaaa
     9541 atcgatctct atcccttcat gaagaagagc ctctacctct tcacgggctg ccggcatctc
     9601 atcacgggtt ctccggtaga tgagtttaac agattcactt cccagtctct ttactgtcct
     9661 tgcgcagtct acagcagtat tgccgccacc gataacggca accctctgcc cggtcttaac
     9721 tgcttcacca ctgttgactg aacggagaaa gcgaatacca tctatcacgc cggggagatt
     9781 ctccccctct acttcaagcg gcaatccctt tggtgtaccg gtaccaatga aaactgcatc
     9841 atagtcattc cttattgtat cgagagtgat gtcttttcct atctctgttc cgcactgaat
     9901 cgtgacgcca agtttttcta tatagcctat ttcctttctc acttcttctc ttggaagcct
     9961 gtattccggt attgcgtatc tcagcatgcc gccaggttcc ggaagggcct caaaaactgt
    10021 tacctcatgg ccttccagag caagatcatg ggcgcaagta agcccggccg gccccgcacc
    10081 gatgacagcc actttttttt cagttttcgt tatctgaggc acctccagtg aatgcacatc
    10141 aaaccgatct acagcaaacc ttttcaggtc acgtatagca aggggttcat ccagttgccc
    10201 tctgttacag actccttcac atggtgcata acaaaccctg ccgcacacag agggaaaggg
    10261 attggtagcc ctgataagca tgtatgcatc ttgaaaacgg ccctcactaa tatgcttcag
    10321 atatccgagc acattagtgt taatggggca ggcatcgaca caggctgctt tgcatggacg
    10381 attctcgcgc ttatctatgg tcgccttgtt gggtatggcc tgaggaaacg gtatgtatgc
    10441 tgcctttctt ggacctgtac ccacgttgaa ttcactcttc atgaccaccg ggcaccctgt
    10501 agagcaggaa ccgcaggccg tacacttttt cgtatcaata tagcgtgttt ttttgaagat
    10561 ggtaacctgg aaacctgtac cttttttctt taccgcaagg ggttttgccc atgacagaat
    10621 ctcaatgttc tgattaagcg acacttccac catcttaggt gagaggacac aggttgcaca
    10681 gtcaagagtg gggaaagttt tgtcaagttg tatcatcctt ccacctatag agggcccttc
    10741 ttcaagcatg gtaaccttga ccccacacga tgcgagttcg agggctgctg taatacctcc
    10801 tattcctcca cctatgacaa gtacattttt ctctgccacc gtgnnnnnnn nnnnnnnnnn
    10861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10921 nnnnnnnnnn nnnnnnnnnn nnntgtaata cctcctattc ctccacctat gacaagtaca
    10981 tttttctctg ccaccgtgac tcctgcactc cgttatctat cttcaaaatc ctaaattcta
    11041 atatgtaaat ccaaaaataa tatctaagcc ccaataacaa aagctcaaaa caattgaacg
    11101 gccacatggc atgctctttg agcatcggaa tattcgagtt tgtttcgtgc ttcgaaattg
    11161 ggatttcgaa tttgccattc tcgtttatcc tttcagtata tctttcaact tatccctcac
    11221 cttgtgcata tcagcaatga tcccgtagtg ggcatattga aatataggcg cttcggcatc
    11281 tttatttact gcaataatac aatctgaatc cttcattcca gctacatgct ggaatgcccc
    11341 gctgacaccc atagccagat acactttcgg tttaaccgtt ttacctgaag taccaacctg
    11401 tcttgctttg ggtagccaaa gtttgtcgat gacaggcctt gaaccagcga tgactgcttt
    11461 gagcatcccg gcaagctcct cgtaatcaga aacttcgtcc ttgttgccaa ctcccctgcc
    11521 aagagagagc aggaactcag ccttcgtgat gtccacatca gatttttctt cctccacaaa
    11581 ttccatgaag gttctcgcag aactcactgc aacatcttcc attgtcccga cagaaggggc
    11641 aacatctttg tcagcagcaa agtctttaaa gcttccgctc ctaactgtaa caatagcagt
    11701 cccattgccg attggacgaa attcaccaaa gatcttgtct gaatagtagc tcttgtaaaa
    11761 aataccttga tccttgtcgc aactattcac atcagtgata atgggcagat caaaataacc
    11821 tgcagcgtaa gaagcaagct ctgttcctga agaggagttc gtaaaaacaa tcatatcagg
    11881 ctttccgcgg tcagccataa ctttttccag aatgttaacc atattgagag ggttgaggaa
    11941 cactgtctct tcgactttca ccttaactat actattgacc aattgctctt tgattccata
    12001 ttcaccctct gggatgataa ggattgcatc ttttccaaga tgagtgcaaa gccctatgga
    12061 atctaaattt ctctcttcga atatgccacg ccttacttct gaaacaacga gtgcgtaact
    12121 cattggtaca cccccttctc cttgagaatt ccaatcaatt ctttgcatgc actttccagt
    12181 tctccgctta agattgtggc accggccttc ttaggcggat atatccatct tgccacttca
    12241 attttggctg taactttttc ctggtaatct gctgatttga agatttttct ctctactttc
    12301 gatgcctttc gaatacccat aattgaaaca taccgtggct cattgattcc tgtctgaatt
    12361 gacatcacgc acgggagcat aacctttacc cgctcctgga gacctccttc aagctctctt
    12421 cttacgactg catgatcttt ttcaaaggaa tcaatgccga tgaccattga aacaaaggga
    12481 agcctcatct tggcagcaag aatgccacca agtgaagcaa actggtcatc ctctgattgt
    12541 acacccgtaa aaatcgcatc aaaagggaca ttctcttttt tcaggaaatt ctgtattaat
    12601 gttgcccggg cattaggatc gtggactatg tcactactct cgacgagaaa accatcttta
    12661 gctcccattg ctatggccct tctcaagacc tcatctgatt cagcatcacc tacagagacc
    12721 gctgttaccg ctcctcctga cttctctacg atgcgcacag cttcttcaac tgagtagttg
    12781 tcccagtcat ttaccttaaa aggcactttg gataaatcga ctgcgctccc ttcaccgata
    12841 attctaagtt cttcttcctg tgtagcaggt accctttttg taaagactat aatattcatc
    12901 tcacaacacc tccatcaata tctcagaaag ctctttcact tttatccctt tctcggtggc
    12961 cgggtcctca agagtcataa cacaaaacgg acagctcgtt acaatctcct cggcacccct
    13021 gttaacagcg tcttgaacac gaacttcagc atttctctga tatgttgctt caacctcgaa
    13081 gaacatcctt ccacccccac cttcacagca gagagaatta tctcgtgacc tcgaaaattc
    13141 gaggagctcc aaaccattaa cagatttgag tatgtctctt ggttcatcaa aaatgccatt
    13201 ctgtttcccc aggaaacatg gatcatgata aatgactttt ttattatatg aaccttttgg
    13261 caccacagta ccgtatgtaa acatcggtgc caacacttca gtataatggg atatcttgat
    13321 cccaaggtcg ccgtattctt ttttgagtgc attcatacag tggggtgaca gcgctataat
    13381 ctctttaact ccatactcct caaatgttgc cctgttctcc tcctgaagtt cctcaaaaag
    13441 gcccgattct cctattcttc ttatctcatt tccgcagcac gcctcttcct cgccaagcac
    13501 tccaaactcg ataccggctt tcttcaatat tgtcgctaca ttaaccggga taaccataca
    13561 gcgcgggtca taagcctgta tacagcaggc gaataataat ctcttcgtct ctgtctcgct
    13621 cacatgggga atctctatat ccattttctc gaaccaggca cccctttttg cgcgcgaacg
    13681 accccaggga ttcttctgaa caaaggtatt ttccaatgcc ttttgtattg tcacaggaat
    13741 tgatccattt tccacgagtg ccgccctcat ttcgattatt acttcagaag gctttacatc
    13801 acggggacac ctgatggtac atgaaccaca tgaagtacaa taccagggta tcgattcttc
    13861 tatcgcattt tgaaactgtt tttttctcat aagcatgcgg acattaaacg gggttctccc
    13921 tgattcagga caactgccag tacatttgcc acattggata caggtcaaca ccctggtctg
    13981 ggtttgggct atcataatgt acctctttga tggaaacctt tatatgtcat tatgtgagtc
    14041 tgtcacactt tatcaatatt ttcagttttg gtaaagcaat ttatatacca tggctaatat
    14101 gataaaacct taataaaaac aagacaatgg ataaggaaga aaaatataca atttcagaat
    14161 tatgtatcat ttcgatacag gtgacgcttt ctaatgcgct attctatggg cttgctacag
    14221 aaaaaattat agttgatccc ttattgaaca actgcgtgtc gtcctgttat ctgtacagta
    14281 taatccatat tgatgcagtt atctgttaaa atagagggat ggatgggccg ggtaatttgc
    14341 gaaaagggga agggggatgc catggcccat ccatagagtt ggcctacttg aatataacct
    14401 taatgcagac caagaagcct tcttccaata aggagcttct cgatctcttt cgtgccttca
    14461 aatatttcaa gaaccttggc gtcacgccag taatgttcta catcgtactc tacgagaatt
    14521 ccactgccac cgtggagctg aatcgcttca tctgccactt taaccgccac ttcggatgcg
    14581 taccattttg acattgccac aagggcagga tctatctctc ctctatctgc cttcaaagca
    14641 gctcggtaaa gcaggctcct tgctgtctca accatggttc ccatctctgc tatctttgtc
    14701 tgtacaactc cgagtgcagc cagagtagca ccaaattgtt ttcttttttt cacatgggct
    14761 acagccttat caagggctcc ttgtgctgtt cccactccaa aagctgcgac tccaagccgc
    14821 gtatgattga agaaatccat aaggtagtaa aagcctttcc cctcttcgcc aaggagattc
    14881 tcccgtggga ctctcatatc attaaaagct acttccccgg tagttgaaca acgaagggag
    14941 agcttcccat gccacgtagt agcctcataa ccagggcttt ttgtatcgac aacgatggcg
    15001 cccagacgct catgtttcct cttggcttct ggagtagtga cacacaatac cacaagaaag
    15061 tcgcttgtgg tacagtttgt gataaaaacc ttgttccctc tcaatacgta gtaatctcca
    15121 tctttcgtgg ccgtggtagc aatcgaggca acgtcgcttc ctgcttcggg ttctgttgat
    15181 gccataccaa ttctccactt tccttcacag acaggcggca ggtacttctt tttttgttct
    15241 tctgtgccgt taaaaagtat gaactgcgca ccaaaaaatg tcgagtcaat ctgatgagtt
    15301 attcctaagt ctacccgggc aaactcttcg aggataaggc actgttcaaa atggccgagt
    15361 ccctgccccc cgtactcttc cggcacaaaa acggcaagaa aaccattttc cgcagtcttc
    15421 ttccaaatcc tatcgtcaaa ctcctctttt gcttccaatt cccgcgctat gtcacggaat
    15481 tctttttcag caaactcctg tgctgccatt tttatatctc tctgttctgc ggtaagttca
    15541 accatgtttt cctccaacat tcctttctgc tatatgagat aatgtatttt ttttctggat
    15601 tattaatttg caaaaataaa gccacactct caaccgttcg gacttgcttc tacctgtgtc
    15661 gagacttcag caggaaggcc gtccctattc cgcctaactg ttgaagctgt ttatacagtt
    15721 taaaccgtta cagtgaacct cccagtccac ccgggaatgg acatggctaa tccacattac
    15781 ccttctaacc ccttaattct gtcttcggtg tttcaccctg catcatagtg tttccttttg
    15841 atacaccagt gagacatcct ttatggagtg gctctgcatt ttctattttc tatattgttt
    15901 gcggatatct tttttcaaaa tctttccctg aggactcttg ggcagttctt tccagaagtc
    15961 cacagatttg ggtttcttga aaccggcaag gtttttaccg caaaaatcct taatttcatc
    16021 ctctgtagcc ttcgcacctg gcttgagaac aatcacagcc ttcacgatct ccccccagtg
    16081 ctcatcaggc aagcctatca ccgatgcctc agaaactgca ggatgcctgt aaagaacttc
    16141 ttcaatttca cgaggataga tgtttactcc cccagagata atcatgtcgc cctttctctc
    16201 aacgatatag acgtacccat cttcatcaaa ttttgccaga tcgcctgtat ggagccaacc
    16261 atttttcagc tttttctcag tctcttccgg catctgccaa taacctatca tcattgcttc
    16321 actacggact atgagttcac ccacttcacc cataggaaca tcgacatcat tgtcgtctac
    16381 tattctgacc tcataattga gggcaggctt tcctgatgat gcaagccgcg ccacactctt
    16441 ctctgttcca tcctggatat ggtcttccgg tctcagcact gttgtaaaag gcccactctc
    16501 tgtctggcca taaagctggg caaagccgca tggaaatatc gttaacgcct tcttgagaag
    16561 ttcgacgggc atggaggatc ctgcatagag aataagcttc agactgctaa ggtcgtactt
    16621 actcacatca ggaacctgaa tgaggctgtg aatcatagtt ggcacaagct ggattcttga
    16681 tatcctctct ttagagatat actcaaggac ctctggaggg ttaaaaccac cctctttctt
    16741 tgtatagatc gtgttagaca gaagtgagtg gcgcacaata ttgtcttcac aggcaacatg
    16801 gtacatagga aaggttatca gcactttttc atcataccct acctgcaaat caatagccga
    16861 cgcaaaagca tcagacatga ggtgtctgtg ggttctcatt gcgccttttg gccttcccgt
    16921 ggtgcccgct gtgaagataa tactctgtac gtcatcttcc aaaatcctga ttctgggctc
    16981 cgcagcatca ctctgagcaa ttaagccctc ataatcttcc caataagctc catctgtttt
    17041 ttgaaggcaa ataaagcttt caaccgattt caattccgat ttgaggttgt ttatggtttc
    17101 aacaaagtct ttatctacaa aaagaaatct gggcgttgag tagtctatga tatctttcag
    17161 ttcccattgc ttcaagtgat tgttgtaagg acagaaaata ccgcctgtct tcgctgcgcc
    17221 ataatatatc tcgatgaatt gatgacaatt gtgaataagg actccaatcc ggtctccttt
    17281 tttgagacct tttttaagga gcccattcgc cagacggttt acccgctcgt ataaggtttt
    17341 gaaatcgatc gaagtttcct ctgagactac tgcttttttt gtgggaaatt tgttcgcatt
    17401 tctggtaagc atttctccaa ctgtcatttt cttcctcctt tcccagagct aacccatcat
    17461 tcagcgattg gcagaagatg taagggagca gttcaattgt agttcccaga aacctcaccg
    17521 tgttcaagga ttgatgcact ttaggctcta aaatttttta tacctaactc tgtcttatgg
    17581 catcaatgtg ttcggtaagt acaacacaag ctgcgggaat gcaatacaga gaaccacaac
    17641 tacaacaagt gaaatcagga atggatatac cccagcgtat attgtgccca ttggtacctt
    17701 ggtgatattt ttcaccacaa aaacacagat agctaccgga ggtattacag accctatgca
    17761 tacagtaaga gccaccaata ttccaatcag caaaggatcg tagcctagtt tttccataat
    17821 cggatagaaa ataggtgttg ccaggatcat aaaggccaga tcatcaataa atgaacctcc
    17881 gatgagatag acgaaaaaaa ccaggatgag cacaaagatt gctggcatct gaatacccaa
    17941 cacccatgca cccagatgat tcgggatatt tgtcactgcg ataaagtggc ccagaatatt
    18001 ggacgttgca acaagtaaaa gaaccatgca tgccgttctc agggcttcct gaacagattt
    18061 cacataaccc ataaaattga gattccgctt aaagagacac aacaggagca ctgtaaatgc
    18121 acctatactg ccggcttctg tgggggtaaa aatccctttc ataagaccga tgatgatgat
    18181 aaaaaagatt acaattggcc atatcacatc aggcaatgtt ttgacccgct ccccccaggt
    18241 aaatctgtca ctctttggtc ctaacccagg atttatcttg caccagccaa atatgaccac
    18301 aacaaagaaa aatgcaatca ggagtccggg tatgattcca gccagaaata acttgccaat
    18361 agataactgg gttacaatac caaagacgat taacaccacg cttggtggta tcaatacacc
    18421 aagcgttcca acagtggcca cgatacctgt tgacagtttt ttagcatatc caaactttgt
    18481 catctcaggc acggcaacac tc
//
LOCUS       DAOOMC010000053        18171 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdus sp. isolate AMR_MDS_2685
            NODE_3176_length_18171_cov_6.137061, whole genome shotgun sequence.
ACCESSION   DAOOMC010000053 DAOOMC010000000
VERSION     DAOOMC010000053.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864760
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdus sp. (wastewater metagenome)
  ORGANISM  Syntrophorhabdus sp.
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae; Syntrophorhabdus.
REFERENCE   1  (bases 1 to 18171)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18171)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,729
            Genes (coding)                    :: 2,708
            CDSs (with protein)               :: 2,708
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 7 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18171
                     /organism="Syntrophorhabdus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3176_length_18171_cov_6.137061"
                     /isolate="AMR_MDS_2685"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2699756"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            666..866
                     /locus_tag="PK125_06910"
     CDS             666..866
                     /locus_tag="PK125_06910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002832300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HPB37873.1"
                     /translation="MSKGIVKWFNELKGFGFITKDDGTDVFVHYSSIQGGGFRSLVEG
                     EAVSFDVVSGPKGPAASNVVKG"
     gene            1087..1383
                     /locus_tag="PK125_06915"
     CDS             1087..1383
                     /locus_tag="PK125_06915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB37874.1"
                     /translation="MTTESIVEMTNVAEFIKIRQQIELLTKQIEYTTASKEATGSIQR
                     FNEATKLLVTLAAMANNDVQKIVIRRLTRQLINLGIKIRTLKGKKRVSRKQPVV"
     gene            1498..1803
                     /locus_tag="PK125_06920"
     CDS             1498..1803
                     /locus_tag="PK125_06920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB37875.1"
                     /translation="MRKGRNTAEIISSDDEFDPMALSESAGMERRLILKATPRSRKGE
                     LMAKGAKEIRCICCHQVKSLAGAEESGDGWICEDCVPEVVQEENILPGDRTQLFGTM"
     gene            1908..>2116
                     /locus_tag="PK125_06925"
     CDS             1908..>2116
                     /locus_tag="PK125_06925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB37876.1"
                     /translation="MRILSVMKYNNYYTVFYETDSNYIREDIFLENTAITKYPKEQFG
                     DYDQFVNTMKEADAGTRFLLEPVEI"
     assembly_gap    2117..2216
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2271..3029
                     /locus_tag="PK125_06930"
     CDS             2271..3029
                     /locus_tag="PK125_06930"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPB37877.1"
                     /translation="MADQTILNIASLTKEYDSLRAVDHISFSVSAGEIVGLLGPNGAG
                     KTTTINMILGILEPTHGSIEIMGRDIRTCGSDIRKYINFAAVYAHLPANLTVIQNLYI
                     FGLLYEIENLKERIRFLLQEFDLERFAHTKAGLLSSGEISRLNLAKAIINEPRLLLLD
                     EPTASLDPSISRLIREKIREYMVRTQTGILWTSHNMNEIEAVCDCVIFLSHGKMLLSG
                     NPKTLPSEYGMKSLEELFIAVAREPLCLAHEVPA"
     gene            3032..3835
                     /locus_tag="PK125_06935"
     CDS             3032..3835
                     /locus_tag="PK125_06935"
                     /inference="COORDINATES: protein motif:HMM:NF013245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPB37878.1"
                     /translation="MTRIFAIYIRQIFLLKSNPTRLATVFLWLVIDIIQWGFITKYIG
                     SFGEATFGFVTVILGAILLWGFMSRIQQGILMAFLEDIWTQNFINFFGSPLKVSEYLC
                     GLVMTSITTGLAGFFAVVTIAGVVFGYNVFRIGVMLVPFMFILLIFGIAMGVFVSAII
                     FRLGPSAEWLAWPIPMMLSLFAGVYFPISTLPPLLHAFAKIIPASYVFESMRSILSAK
                     AFPGDLAVNLLIGAFLALVYLFAASRVFIYIYRKNLESGTIARFNAEAL"
     gene            complement(4289..5806)
                     /locus_tag="PK125_06940"
     CDS             complement(4289..5806)
                     /locus_tag="PK125_06940"
                     /inference="COORDINATES: protein motif:HMM:NF010184.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HPB37879.1"
                     /translation="MTREVCFDNEKYLKEQTAEILKRVKMFNDKLYLEFGGKLLYDFH
                     AARVLPGYDPNCKMRLLAELKDKADIVLCIYAGDIERKKIRADFGITYDSDALKLIDD
                     LRSWDINVLGVNITRFENQPAAVVFKNKLERRGIKVYTHHFTKGYPTDVDLIVSDEGY
                     GANEYIETDKPLVVVTGPGPGSGKLATCLSQLYHDHKRGIKSGYAKFETFPIWNLPLK
                     HAVNVSYEAATADIKDFNLIDPFHLEAYKQIAVNYNRDVEVFPVLKRILERITGNTSF
                     YRSPTDMGVNRAGFAITDDRIAGEAAKQEIIRRYFRYRCEYAMGFTDRETVQRVELFV
                     KDFKLEPEYRRVVAPAREAARLAEESKKGNEGIYCGAAIELKDGTIVVGNNSPLMHAS
                     TALVINAIKHLAGIPAKIKLLPDYITESVSNLKTEVLREKAVSLNLEEAFIALCISAK
                     TNPAAQMALESLKELRDCEMHITHIPTPGDEAGLRRLGVNLTSDPNFSSKDLFVF"
     gene            complement(5979..6371)
                     /locus_tag="PK125_06945"
     CDS             complement(5979..6371)
                     /locus_tag="PK125_06945"
                     /inference="COORDINATES: protein motif:HMM:NF037472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rap1a/Tai family immunity protein"
                     /protein_id="HPB37880.1"
                     /translation="MKKIMFAVIVAFILTLACQFTSYATDGNTLLESCTAAIQKMDGK
                     DKVSGVKVGYCYGYVQGLIDMNAFYKVSGAPPLFCSPQTISNGEGARIIYNYLQNHRE
                     RLGEYAGILAVDAYVEAFPCSDLKLSLR"
     gene            complement(6638..6844)
                     /locus_tag="PK125_06950"
     CDS             complement(6638..6844)
                     /locus_tag="PK125_06950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB37881.1"
                     /translation="MIIRVMYKDYKYDYVNARKLDQLIASQKIIKFVRPSEDTWIHID
                     RDPIRGTGGSGYDGPERRSSHLIP"
     gene            complement(6921..8333)
                     /gene="ppcB"
                     /locus_tag="PK125_06955"
     CDS             complement(6921..8333)
                     /gene="ppcB"
                     /locus_tag="PK125_06955"
                     /inference="COORDINATES: protein motif:HMM:TIGR02724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylphosphate carboxylase subunit beta"
                     /protein_id="HPB37882.1"
                     /translation="MDLRDFIDMCERAGQLKRIKAEVDWNLEISHVSKLVEEKGGPAL
                     LFEKVKGYGSPVFTGAFATTQRLGLILGKGTDLSMVQLTKEWMNLAIGELIPAQEKKD
                     GPIFENIVDGEKIDAYMFPSPKFYELDGGRYFGTTVFLVMKDPETDEVNLGTYRMQLL
                     DKKTLGVQILKGKRGDIILQKYRKMGKKMPVCAIIGGDPLLLLAGAAMVARASEYDVV
                     GSLRGSPVEIVRGRCTGLPIPAHAEIVLEGEIDPDILREEGPFGEFIGYYTDELIKPI
                     PKPTLEVKCIYHRNNPILWETSAGRPVTDVHMLLSFTQSAMLWTELARMEIPGIKSVY
                     MPPEAAGRFWTIVSVEQMYPGHAEQVGSAVIATNTASYCCKGVIVVDHDVAADDLPRV
                     WWALGTRFSPARGTQIINRGRATPLDPALEPGTDKLITSRIILNATIPFEWKIKPTEI
                     KLSEAMLDRVTSRWKEYGLE"
     gene            complement(8719..9666)
                     /locus_tag="PK125_06960"
     CDS             complement(8719..9666)
                     /locus_tag="PK125_06960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB37883.1"
                     /translation="MAKITFINLSLALAVLLVGIQGCGPVFIHMSPEKVTACTERNVS
                     IKQGETTRNQVHELLGAPMMSSRLWQVDVHHFSDKKLTPGLMTMFLVPLFPVAEKVNW
                     DGYVLVTYDDNNRVSGFASGKVAEPNRDGLLMQANDMTLRYIYNKSSHRSELALLAGG
                     QRLIAGLKSLHKANTCTLVVACDQDRGCPDRFSVVDESATIVDIDPRFIVRVDFYKGG
                     IPIPVMYPIMLAAGKKRLAISNNGLMDKSETTLNCTDEDVRYAIIQVKAPSGTKRQTR
                     LHITENIPEDLLAYNIVVWRASDAILLGESEKAGETFNP"
     gene            complement(9848..10516)
                     /locus_tag="PK125_06965"
     CDS             complement(9848..10516)
                     /locus_tag="PK125_06965"
                     /inference="COORDINATES: protein motif:HMM:NF015321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HPB37884.1"
                     /translation="MGERISLFTTLESMVLLCSLALYKSLHIVVGVDASIRNDSSGIV
                     AVTKQGDRIVLVRNQKWQPSKKNPIDFEASLERYIRELNDQFTIKACYYDPYQFHRSA
                     MTLAKDHIKMVEYPQTLDRLTQMSQNLYDLIIGRNLLLYPDKEVKAHAQKAIATETPG
                     GWRIVKKQTSHKIDLIIALAIACQGAIDMKTGHREWIRTDAPDLPDEDEENEDGWENV
                     SGLH"
     gene            complement(10681..11343)
                     /locus_tag="PK125_06970"
     CDS             complement(10681..11343)
                     /locus_tag="PK125_06970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB37885.1"
                     /translation="MLKNNILLSTIAEKIGLSINFAHARHSIFPGNKQHHRRIHRLKK
                     ADKPYLLVVGYMKTIETGIGSLNPIVQVIKTYLAWAQGGLAMTNGRKEDEILRQVADS
                     IVEDFKKEGEEYTPQQEAEFRAFVAGLVNAVPPEEEDIWQTIEILKKTIEIKDKKNAL
                     GAVTTFMLQFISGCESTSGALGLLPLLVETREAIKADRFDEASELLMTFLRRSRELVR
                     SM"
     gene            11409..12119
                     /locus_tag="PK125_06975"
     CDS             11409..12119
                     /locus_tag="PK125_06975"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPB37886.1"
                     /translation="MGCNTSKTLSLVVLLLIIIPASANSFAGSDEIIYDRNISPSIEW
                     LQEGRWLLKPGKNRDPIDAFSRSIELDPNNFRSYINRGNAYMMQKQYDLAIKDYTTAI
                     ELQPKAGEAYNNRGIAFGMKQQHERAIDDFAMAIILNKNDFKAYVNRGNSYRMKKQYN
                     MAIKDYTRAIELKPRHATTYYNSACVYSLMNSTAEACKFLKKSIDNGFNTWSHIKSND
                     CFDNIRNSACYIEIMSNK"
     gene            complement(12168..13217)
                     /gene="hisC"
                     /locus_tag="PK125_06980"
     CDS             complement(12168..13217)
                     /gene="hisC"
                     /locus_tag="PK125_06980"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016289542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HPB37887.1"
                     /translation="MWSDCIRSIEPYRPGEQPQIGQIIKLNTNENPYPPSPAVIDALR
                     EAANESLRLYPDPEGMVLKQTIADIYNVSQENVFLGNGSDEVLAHTFLGLLKHDLPIL
                     FPDITYSFYPVYCALYNISYQTVALDEHFQVNVEDYGQPNGGIVLSNPNAPTGSALSP
                     ALLRYLIEHSRDSVVVIDEAYVDFGAETALPMVKEFSNVLIIQTLSKSRSLAGLRVGF
                     AIGDRNLIEALNLVKNCFNSYPLGRLALAGAEAALKDRNYFEKTRQQIIKSRDLLAEE
                     LVSLGFHVVPSKANFLFVKHGSCSAREILQQLRQRKIVVRHFPQPRIIEYLRISIGTP
                     EECAELVRALQEILG"
     gene            complement(13381..14109)
                     /locus_tag="PK125_06985"
     CDS             complement(13381..14109)
                     /locus_tag="PK125_06985"
                     /inference="COORDINATES: protein motif:HMM:NF024938.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HPB37888.1"
                     /translation="MKDAGKRKKSDAHFTEQTDIIANALKEGRHILSEHESKRVLGAY
                     GIPVTKEREAHNRKSFKDALIEIGFPLAVKVSSPHISHKTERGLVHVDIRNQREAMAA
                     YRKITDKNEAEGFPVLVQEMVSGERELMIGLKRDLQFGPCVAFGLGGIFTEIFRDICV
                     RIAPVEKAEALRMMRDFRAHKILNAFRGMPGADMDELARMIMVVGKIGLEEKRIMEVD
                     INPVILDDDGKPVAVDALIALHEM"
     gene            complement(14106..15518)
                     /locus_tag="PK125_06990"
     CDS             complement(14106..15518)
                     /locus_tag="PK125_06990"
                     /inference="COORDINATES: protein motif:HMM:NF039222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HPB37889.1"
                     /translation="MSKGMSGKTIFDELDPIFHPRSVAVLGASAKQGKIARVLMDRFL
                     EMGFKPLYPVNPRESEIMGLRVFHNIKDVPGPVDLAIILTPTDAALAAVKDCVTKKVK
                     TIVVTTSGFGEAGEQGRKVQQEMVHIARKGGARIIGPNCVGIYCPSSKLPFLCQAGKD
                     SGIVGVVSQSGFFADFITQTACANNIKFSKAISCGNEADLNAVDFLEYLGEDPETELI
                     AGYIEGVKDGRRFYELSREISKKKPIILWKGGLTEAGARAAVFHTGAIAGSRAVWEGA
                     LRQAGIVDARSFEEVFDYLYAFYNQPLPKGKRVGIISGPGSTAVGTTDICLELGLELP
                     HFSKETVEKLRRIIPPVGGSVNNPIDLSLASLVNPLIHKDAIDAVARDKNIDMLLLIT
                     VIGGEQLREIVLDATRNVENKKPLVVTLMGGTTQEVAQDFPLMLGSGISVYPDAARAA
                     KVLKKLWEYAKFRAGGSICI"
     gene            15745..16296
                     /locus_tag="PK125_06995"
     CDS             15745..16296
                     /locus_tag="PK125_06995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB37890.1"
                     /translation="MKAIYCMYCLLFAITIISPAATAQGAEWFSIGEDRAGNELFFDL
                     ESLVQHSGVIKAWMKAIYSREGRKERIKERVMKKASVERYDRLSYALELQEINCGRRE
                     FRVLAYADYSSDGRILHSSIIDHHPAEGWEPIAPDSMGEVIDRLVCPSSPLQKKKLPG
                     IIERGGFIKINGSDLISGVKSPL"
     gene            16486..17673
                     /locus_tag="PK125_07000"
     CDS             16486..17673
                     /locus_tag="PK125_07000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB37891.1"
                     /translation="MYITSFAHREELFQMVQRWFCNQLEPTDGHRVTKILICDGFILG
                     ETLNDLVRLLISTFSDETYRTQRLHFKGELRDIICQSSHERNDRTTELIESYKNNPDF
                     YFTEAPIDGSAYLDTMARLLAVYRIKRPRRIAEKANRYIANHIFRMVQNRARQMAEER
                     AQQIGVSLEHLLTTEEDMEREFTLAEEMIAGAFRQGYARFEKPAVTINDVGGIKYIAN
                     EESLHAIENFFLTHPDFKVFERSEHTGNYRAKSLILEVPWDKEKVCRSYLETHAWKKY
                     LKRGISEAELQKGLEHLVEDAEPRICIEVTLTTFDDLVESEFGRGIHEERIITQRGQP
                     VYVGHIPLNVGFLVEYLFAVAVSPRIHIDRLPIKLWGRYLPDTISYHIRQLYYLPEHD
                     ALY"
BASE COUNT         4975 a         4159 c         3862 g         5075 t
ORIGIN      
        1 ttgattaaat agtatagagc ttttttatct ttggcaacag aatttaactg tggttgggga
       61 tgtacaaaga tacacgcatc agttccactt tcaacttacc caaactacgt aggaaatctt
      121 taagaaaact ccattaatgt acaacctttt tgctcaccat tgatgaactg acatcacagg
      181 caatcaagcc ggcccgccgg tgttgtattt tctggctgat ttatgggttt tgttcgatcc
      241 ctgatataat acatagtctt ggaatctacg tttgccagtc ggtgaagcct ggcaaggttc
      301 ctggctgttg ttttaagtgc ttcctgtgat cataacttga gaataatggc agcaccagca
      361 ttatcatcat ctttcatgat cctggcgagt acctggccgg actgaatgac atggccagta
      421 tcaagcccgt ataaccctgg tcacacactt taaccgccag atggtggtca ttcgttatcc
      481 taattaatta tggaccataa aaacggcaaa ttatttgaaa tatattgaaa ttctattatt
      541 tttatgccat aataaagcag aagattcgga agcacctgtt aatagtaacc gcaaaaacca
      601 tcatttttgt cgtctcttca taacacagcc cgtatgcttc aaataaaaga agagaggtag
      661 taagtatgtc caaaggtata gtaaagtggt tcaacgagtt aaagggtttt ggttttatta
      721 ccaaagatga cggcactgat gtgtttgtcc actactcttc cattcagggt ggtggcttca
      781 ggtcacttgt tgaaggagag gcagtaagct ttgacgtagt cagcggtcca aaaggtccag
      841 ctgcatcaaa cgtagtgaag ggataaaaac aattaagaca ggtcgagcag gtcttggcct
      901 gtcttcctgc ttgcagactc cacagatccg ttcgtacggt acacacgttg gtgaaacatg
      961 cttcaaaaaa gacgccctat taagcagctt ttcatacgca ctttccagca cagctcaaca
     1021 caggatcggc tcacagggtt gtcaatgtaa aaataccatc aaccagaata tggagggaac
     1081 acaggcatga caacagaatc gattgtggaa atgacaaatg tagctgaatt cattaaaatc
     1141 aggcagcaaa tagagttgct cacgaaacag atagagtaca caactgcatc gaaggaagct
     1201 accggatcaa ttcagagatt taacgaggca acgaagctat tagtaacgct tgcagctatg
     1261 gcaaacaacg atgttcaaaa aattgttatc agacgcttaa ccagacagct tatcaatctt
     1321 ggaataaaaa tacgaacatt aaagggcaag aaacgagtat ccagaaaaca acctgttgtt
     1381 tgacaatgta ctgcacagcc tgaaaatctg aaaatgatat atgtatcaca atgtgaagat
     1441 gtaggctcag ggattttttc agtttctgat tggtctttca caagaaggag taaggttatg
     1501 agaaaaggac gaaatacagc agagattatc agcagcgatg atgaatttga ccctatggct
     1561 ctatcagaga gcgctggaat ggaaagaagg ctgatactca aggcaacacc ccgatcgagg
     1621 aagggagagc ttatggcaaa gggagcaaag gaaatacggt gtatttgttg ccatcaggtt
     1681 aaatcactgg ctggagctga ggaatccgga gatgggtgga tctgtgaaga ttgcgtgcct
     1741 gaggtggtgc aggaagaaaa tatcttgcca ggggacagga cacaattgtt cgggaccatg
     1801 tgaaaaggtg atatttcgac aataacgagg tccggagttc aagaaacgga ggttaaacag
     1861 gtacgattaa ctgaagcatg aacatttata ggagaaattg gtaagtaatg agaatactat
     1921 cagttatgaa atacaacaat tactatacag tcttctatga aacggattcg aattacatca
     1981 gggaggatat ctttctagaa aatacggcaa taacgaaata tccaaaagaa caatttggtg
     2041 actatgatca gtttgtcaat accatgaaag aggctgatgc tggcaccaga ttccttcttg
     2101 agcctgttga gatagannnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2161 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnatgt
     2221 caattcatgg tattgtttct tagattaaga aaatattacc atggaaattc atggctgacc
     2281 aaacaatcct gaacattgcc agccttacaa aagaatatga ttctctgaga gcggtcgatc
     2341 atatatcttt ctcagttagc gcaggcgaga tagtcggcct tcttggccct aacggagcgg
     2401 gcaaaacaac cactatcaac atgatcctcg gtattctgga gcctacccat ggttctattg
     2461 aaatcatggg acgagatatc cgtacctgtg ggtcagacat ccgaaaatat atcaatttcg
     2521 ctgctgtcta tgcacatttg cctgccaatc tcactgttat ccaaaacctc tatatctttg
     2581 ggttactcta tgaaatagaa aatctaaagg aaaggatcag attcctcctg caggaatttg
     2641 atttagagcg gttcgctcat acaaaagccg ggctactttc ttcgggtgag ataagccgcc
     2701 tcaaccttgc caaggcaata atcaatgaac cacggctgct ccttcttgac gaaccgactg
     2761 cgtcgctgga tccatccatt tcccgcctta tcagggaaaa gatacgagaa tatatggtca
     2821 ggactcaaac tggaatcctg tggacatcac acaacatgaa cgagatagag gctgtctgtg
     2881 attgcgttat atttttgtca cacgggaaaa tgctgctcag tggcaaccca aaaacactgc
     2941 catcagaata tggcatgaaa agcctggagg aacttttcat agcagtggca cgggaacctc
     3001 tctgtcttgc gcatgaggtg ccagcttgag cgtgaccaga atttttgcaa tctacatcag
     3061 gcagatattc ctcctcaaga gcaaccccac gcgccttgca actgtttttc tgtggctggt
     3121 catcgacatt atccagtggg gatttattac taaatacata ggctcttttg gggaagcaac
     3181 cttcggattt gtcacggtaa tcctcggagc tattctcctg tggggattta tgagcaggat
     3241 ccaacagggt atactgatgg cctttctcga agacatctgg acacagaact ttataaattt
     3301 tttcggatca ccccttaaag ttagcgagta cctctgtggt cttgtaatga ccagcattac
     3361 aactggcctt gcaggtttct ttgcagttgt taccattgct ggggtagtgt tcggctacaa
     3421 cgtttttcgg attggggtga tgcttgtgcc attcatgttt attctactca tctttgggat
     3481 agccatgggc gtttttgtgt cagccataat ttttcgcttg ggaccctctg ccgagtggct
     3541 tgcttggccc attcccatga tgctttccct ttttgcaggc gtttactttc ccatttccac
     3601 cctgccgcct ttgctccacg cttttgcaaa aataattcct gcatcgtatg tgtttgaaag
     3661 tatgcgcagc atcctttcgg caaaggcatt tcccggcgac cttgctgtaa atctacttat
     3721 aggagctttt ctagcacttg tctatctctt tgccgcatcc cgggtcttta tctacatcta
     3781 ccggaagaat ctggagagtg gaaccattgc gcggtttaat gctgaagccc tataaagtaa
     3841 gccaggcttt gattttcctc tcaaagaact gatcgctttt ttcctgacat aattcacaat
     3901 tcttcataat cctttcgtta tcttttcaca ttccaccctt atatttatta cagattaaaa
     3961 acggaagcaa aacaagggcg tgaagatggg tgcagcggct attatggcaa aaaaaatggc
     4021 agttaactgc acaagacgtt tgcctggtgt ggtgttcgtg aagaaagttc acggtaagta
     4081 aacaggctta aggtccaagt gcctctcctc ccccgggttt ccgacaaatt tttcccctcc
     4141 ttctaatccc tgttggaacc ccgggggttc cggtctttaa tgatgagtct ggtgggttat
     4201 cattgcgcag ataatctttt tttatgggca cgtactggag ctcatcataa cgtgcaaaaa
     4261 ccagcacgcg gcccgaataa tccggtcctt aaaagacaaa cagatctttc gatgaaaaat
     4321 tcgggtcact cgtcagattc acaccaaggc ggcgcaatcc tgcttcgtct cccggtgtag
     4381 ggatgtgggt aatatgcatt tcgcaatccc ggagttcttt aagactctca agggccattt
     4441 gagctgccgg gttagttttg gcactgatgc agagggcaat gaatgcctct tctaaattca
     4501 ggcttacagc cttctctctc aacacttcgg ttttcagatt agatacagac tccgtgatgt
     4561 agtcaggcaa cagcttgatt ttagccggga tgccggccag gtgcttaatt gcattgatga
     4621 caagggctgt ggacgcatgc atgaggggag aattatttcc cacaacgata gttccgtctt
     4681 tcagttcaat agccgctccg cagtaaattc cttcattgcc ttttttgctt tcttctgcta
     4741 atctggcagc ctctcttgcc ggcgcaacaa cccttctgta ctccggttcc agtttgaaat
     4801 ctttcacaaa gagctccact cgctgaacag tctccctatc ggtgaatccc atggcatatt
     4861 cacaacggta acgaaagtat cgacgaataa tctcctgctt tgccgcttcc cctgcgattc
     4921 tgtcgtctgt aattgcaaag cccgccctat tcacacccat atcggttgga gacctgtaga
     4981 aggatgtgtt gcctgttatt ctttcgagaa tcctttttag aacagggaaa acctcaacgt
     5041 ctctgttata attgacggct atctgtttat aagcctccag atggaaggga tcaataaggt
     5101 tgaaatcttt tatatcggca gttgcagcct cgtaggacac attcactgca tgcttgaggg
     5161 gaagattcca tatgggaaag gtttcaaact tggcgtaccc tgatttaatg ccccgtttgt
     5221 gatcatgata aagctgggaa aggcatgttg ccagtttgcc gcttcccggg ccagggccgg
     5281 taacaacgac caatggtttg tcagtctcaa tatattcatt cgcgccgtag ccttcatcgc
     5341 tcactatgag gtccacatca gtgggatatc ctttcgtgaa atggtgggtg taaaccttta
     5401 taccacgccg ttccagcttg tttttaaaaa ccacagcagc cggttgattc tcaaaacggg
     5461 taatgttgac gccaagaaca ttaatatccc aactcctcag atcatcgatc aatttaagcg
     5521 catcagaatc ataggttatg ccgaaatcag ctcttatttt tttcctttca atatcaccgg
     5581 catagatgca taagactatg tcagctttat cctttagctc cgccagaagc ctcattttgc
     5641 agtttggatc ataaccaggc agcacacggg cagcgtggaa atcatagaga agctttccac
     5701 caaactcaag atagagtttg tcgttaaaca ttttcactct cttgagtatt tctgcggttt
     5761 gctcctttaa atacttctca ttatcaaaac agacttcgcg ggtcataaca ctcacaccag
     5821 agaactcctt taaaggtaga ttggtatcaa gttgaatcca gctatcttaa ttcatttatg
     5881 aagatttaaa cagaaaaaaa tacaggcgta aatactttta caggaaagat atctgctgta
     5941 agtacaaaaa aacccggatt tctccgggtt ttcaactgtt atcgtaaact taattttaaa
     6001 tcactgcacg gaaaggcttc aacgtatgca tcgacagcaa ggattcccgc atactcacca
     6061 agtctttctc gatggttctg caggtaatta tatatgattc gggctccttc accatttgaa
     6121 attgtctggg gtgagcaaaa caatggtggc gctccagaca ccttgtaaaa agcgttcatg
     6181 tctatcagtc cttgtacata cccgtaacaa taacccacct tgacaccgct cactttgtcc
     6241 tttccatcca tcttttgtat agctgctgtg cagctctcca gaagtgtatt tccgtcagtt
     6301 gcataactgg taaattggca tgctaacgta agaataaagg caacaatcac agcaaacatg
     6361 atttttttca tctttctgac ccccttctca gtcatcggtg atttctcttt gttccctgct
     6421 tttctctaat gtcatcggcc ataacacagg ggacttaaga cgatcggtac gtttactgac
     6481 ctgtcaccta ttcaacagac atttctacgc tggaaagaca atcgctgggt ttaaaactga
     6541 gtttgtatgg ttgttttgaa agaaatcacc tggttttgct atgggaaagt caaaatgtgg
     6601 gtgattgttg taaaaaaagg aggtgtgaca aaaggcttca gggtatcaga tgagacgacc
     6661 ttctttctgg tccatcatag cctgacccgc cagtgccacg aatcggatca cggtcaatgt
     6721 gaatccacgt gtcttctgag ggacgtacaa actttattat cttctgggat gcaataagct
     6781 ggtcaagttt gcgggcatta acataatcat atttataatc tttgtacata acacgaataa
     6841 tcatcaagcg ctccttacga ctgtcaaaac caaagcacag cttattatag cagaaatggc
     6901 ttggagagta cagagattgt tcattccagt ccatactctt tccacctcga cgtcaccctg
     6961 tcaagcatgg cctcggaaag ctttatttca gtcggtttta tcttccattc aaaggggatg
     7021 gttgcattca ggatgattcg tgaggtaatg agtttgtctg tacccggttc aagggctggg
     7081 tcaagggggg ttgctcttcc ccggtttatg atttgtgttc cccttgcagg actaaacctg
     7141 gtaccaaggg cccaccagac ccttgggagg tcgtcagcag caacatcatg atccacaaca
     7201 ataactccct tacagcagta tgaagctgta ttggtggcta tgactgcaga gcccacctgt
     7261 tcggcatggc caggatacat ttgttcaaca gagacaatgg tccaaaaacg tccggctgcc
     7321 tccgggggca tatatacaga tttgatgcca ggaatctcca tcctggcaag ctccgtccat
     7381 agcattgcgc tctgagtaaa tgacaggagc atgtgaacat cagtcaccgg ccttcctgca
     7441 ctcgtctccc agagaatcgg gttattcctg tggtaaatgc atttgacctc aagagtaggc
     7501 tttgggatgg gcttgatgag ctcatcagtg tagtagccta taaattctcc aaacgggccc
     7561 tcttcccgca gtatatcagg atcaatttca ccttccaaaa cgatctctgc atgagcagga
     7621 atcggcaggc cggtacaacg gccccgaaca atttctacgg gtgatcctcg caatgatccc
     7681 actacatcat actcgcttgc cctggccacc atggcagccc ctgcgagaag gagcaacgga
     7741 tcgcctccga taattgcaca gaccggcatt ttcttgccca tcttcctgta tttctgtaaa
     7801 attatatcgc ctcttttacc cttgagaatc tgtactccca gggttttctt atcaaggagc
     7861 tgcatacggt atgtaccaag gttaacctca tctgtttcag ggtctttcat gacaaggaag
     7921 acggtggttc caaagtatcg cccgccgtct aattcataaa attttggtga agggaacatg
     7981 tacgcatcaa tcttttcacc gtcaacaatg ttctcgaaaa ttgggccatc ttttttttct
     8041 tgggcaggta tcaactcacc aatggcaagg ttcatccatt ctttcgtaag ttgcaccatg
     8101 gaaagatcgg ttccctttcc cagaattaat ccaagcctct gggttgttgc aaaggcgccg
     8161 gtaaaaacag ggctcccata tccctttact ttttcgaaga gtagtgcagg tccacccttt
     8221 tcttccacta actttgaaac atgggatatc tcaaggttcc agtctacctc ggcttttatc
     8281 cttttgagct gccctgccct ctcacacata tcaataaaat ctcgtaaatc cattcacaat
     8341 ctccttgctt tgtactctat tctctggatt ttgcgtcgcc cctccaccag tagagctgtt
     8401 aaaacctccc cttcaagctc atggcattaa cgttatattt ttgtattcta taattcggca
     8461 caaagcttgt caaagaaaaa cctgctgaaa taccttaaat aaaaggaaaa cctaagctta
     8521 gccctttttt gttttcgatg ttaaaaaaat aattgagttt cgagtagaac ggtttctccc
     8581 gatgcaacag gttgaggttt ggaataatca aaagaatata ttccttcacc gttggcagaa
     8641 tgtctataaa atgaagcgtt gactatttac gaggtacacc taccaacaca tcattgggct
     8701 atttctgcaa ggtaatgatt acggattgaa agtttcgcct gctttttctg attctcctaa
     8761 caggattgca tcgctcgctc gccagacgac gatattgtag gctaacaaat cttctggtat
     8821 attctcagta atatgcagcc tggtttgacg cttcgtccct gaaggtgctt ttacctgaat
     8881 gatggcgtaa cgcacatctt cgtctgtgca atttaaagtt gtttcagact tatccatcag
     8941 gccattattg ctaatagcca gccgtttctt ccccgcggca agcataattg ggtacatgac
     9001 cggtatgggg atgccacctt tgtagaagtc aacccgtaca ataaaacgtg ggtcaatgtc
     9061 aactattgtc gcactttcgt ccacaacgga gaacctgtct gggcagccac gatcctgatc
     9121 acaagctaca accaatgtgc aggtattcgc cttgtgcagt gacttgaggc cagcaatcag
     9181 acgttgtccg ccagcgagta aagccagttc gcttctgtgt gaactcttat tatagatgta
     9241 tctcagggtc atatcattgg cctgcattag cagcccatcg cggtttggtt ctgcaacctt
     9301 gccggaagca aatccgctta ccctgttgtt atcgtcgtag gtgaccagta catagccatc
     9361 ccaatttact ttctcggcca caggaaacaa aggaacaaga aacattgtca tgagccctgg
     9421 agtaagcttt ttatcgctga agtgatgaac gtccacctgc cataagcggc tactcatcat
     9481 cggagcgcca agcagctcat gaacctggtt gcgcgtagtt tcaccttgct tgatagaaac
     9541 attacgttca gtacaagcag tgaccttttc tggtgacata tgaatgaaga cagggccaca
     9601 gccctggata cctacgagaa gaacggccag cgctaaagac aaattaataa atgtaatctt
     9661 tgccacgatt acccctgcta ccaataattt aacatagatt ataccatgaa cagccagatt
     9721 tacggactga aaattggcat gcctgcattc ctttcagtct gtaatacagt atttgccaca
     9781 gaacacaaga aacaacattt caggaaggag tcattgaaga tcaaagaaaa atatttggat
     9841 ctcgagatca gtggagacca cttacatttt cccagccatc ctcgttttct tcatcttcat
     9901 ctggtaaatc gggagcatct gttctaatcc actcacgatg ccctgtcttc atatcaatcg
     9961 caccctgaca agcaattgct aatgcaataa tcaaatcgat tttatggctt gtttgttttt
    10021 taacaattct ccaccctcca ggcgtttctg ttgctattgc tttctgagcg tgtgctttca
    10081 cttctttgtc aggatagagc aagaggtttc taccgattat caaatcatat aagttctggc
    10141 tcatttgtgt gagcctatca agagtttgtg gatattcgac catctttata tggtctttag
    10201 ccaatgtcat agcagaacga tggaattggt aggggtcata ataacaagcc tttatggtga
    10261 attgatcgtt aagttctctt atgtaccttt caagacttgc ctcaaaatct attggattct
    10321 tcttactggg ttgccacttc tgattacgta caaggacaat tctatctcct tgctttgtta
    10381 ctgcgacaat accgctacta tcgttcctga tagaagcatc aacaccgaca acgatgtgta
    10441 atgatttata aagagccaag ctacataaga ggaccatgct ttcaagggtg gtgaatagac
    10501 ttatcctttc acccacagca tgtgtatttt tgtgccacgg gattacccat gggaatctac
    10561 cccctccctt gatcgttgaa tctgttctat cctctttttt aggtcacaag tagattaaat
    10621 cacaacagga cagtgtgtgg aatcgtcttc tcaagagagg aaaacctgtt gtgttttgca
    10681 ttacattgaa cggacaagct ccctgctccg gcgcagaaaa gtcatcaaca actcactcgc
    10741 ttcatcaaag cggtcagcct ttatggcttc tctcgtctcg acaaggaggg gcagtaatcc
    10801 gagcgcccct gatgtagact cacaccctga gatgaactgg agcataaagg tggtgacagc
    10861 tccaagggcg tttttcttat ccttgatctc aattgttttc tttaatatct cgatagtctg
    10921 ccatatgtct tcctcttccg gcggtaccgc attgacaagt ccggcgacaa atgccctgaa
    10981 ctcagcctcc tgttgaggag tatattcttc accctctttc ttgaagtcct caacaatact
    11041 gtctgccacc tgccgtagta tttcatcttc ctttcggcca tttgtcatcg ccagccctcc
    11101 ttgtgcccat gccaggtacg tttttatcac ctgcacgatc ggatttaacg aacctatccc
    11161 ggtttcaatg gttttcatgt atcctaccac aagaagatag ggtttatcag cttttttcag
    11221 gcgatggatt ctcctgtggt gctgtttatt tccagggaaa atgctatgcc gggcatgtgc
    11281 gaagtttatt gacaggccta tcttctctgc tatagttgaa agtagaatat tatttttcag
    11341 cacatttttt cacttacata ccttcctttg tacttgaaaa ataatatccg atttagtgaa
    11401 aatcgtttat gggttgtaac acttcaaaaa cattatcatt ggttgttctt ctccttatca
    11461 tcatacctgc atcagcaaac tcatttgctg gaagcgatga gatcatttac gacagaaata
    11521 tcagcccatc aatagaatgg ttgcaggaag gccgctggtt acttaaacca ggcaagaatc
    11581 gagaccccat cgacgctttc agcagatcga ttgagctgga cccaaataac ttcagatcat
    11641 acatcaacag aggaaatgcc tatatgatgc aaaaacaata tgacttggca ataaaagatt
    11701 acacaacggc cattgagtta caaccaaagg ccggcgaggc ttacaacaat cgtggaattg
    11761 catttggtat gaagcaacag catgagagag caattgatga ctttgctatg gcaatcattc
    11821 taaataaaaa tgatttcaag gcctacgtca atcgtggaaa ctcttatcgg atgaaaaaac
    11881 aatacaacat ggccatcaaa gattacacca gggccattga gttgaaacca agacacgcca
    11941 caacctatta caattcggcc tgcgtctact ccctgatgaa tagcactgcc gaggcttgca
    12001 aattccttaa aaaatcgatt gacaatggat tcaatacgtg gtcccacata aagtcaaacg
    12061 attgttttga taatatcagg aattcagcct gttacattga aatcatgtca aataagtaga
    12121 cattgtagca aagattaagg gcaataactg ccggcagcaa gaaggtctta gccaagaatt
    12181 tcctgaagag ccctgacaag ctctgcacac tcctcgggcg tcccaattga aatccttaaa
    12241 tattcgatga ttcgcggttg tggaaaatgc cggactacaa ttttcctttg tctgagttgc
    12301 tgaagaattt ccctggcaga acatgaccca tgtttaacaa acagaaagtt tgcctttgac
    12361 ggaacaacat gaaaacccag agaaacaagc tcctcagcga gtaaatcccg gctcttgatg
    12421 atctgctgac gggtcttttc aaagtaattc ctgtctttga gtgctgcctc tgctcctgca
    12481 agggcaagtc ggcccaaagg gtaggaatta aaacagtttt ttacaagatt gagggcttca
    12541 ataagattcc tgtcgcctat agcaaagcca accctcagcc ctgccaggga tcgtgacttt
    12601 gaaagtgtct gaataatcag cacgttggaa aattccttaa ccatgggaag tgcggtttca
    12661 gccccaaagt caacataggc ctcgtcaatc acaacaacag aatccctgga atgctcgatc
    12721 aaataccgaa ggagggcagg actgagtgca gacccggtgg gggcattcgg gttggatagc
    12781 acgatgccac catttggttg cccatagtct tcaacattaa cctgaaagtg ttcatcaagg
    12841 gctacagtct gatagctgat attgtataaa gcacaataaa caggatagaa actgtacgtt
    12901 atatcaggaa agagaatcgg caggtcatgt ttcagcaacc caagaaatgt atgggccaaa
    12961 acctcgtcag acccgtttcc aaggaagaca ttttcttggc tgacgttgta aatgtctgct
    13021 attgtctgtt tgaggaccat tccctcggga tccgggtaga ggcgaaggct ttcattggca
    13081 gcctctcgca atgcatcaat aacagcgggc gatggggggt agggattttc attggtattc
    13141 agcttgatta tctgaccaat ctgcggttgt tctcctggcc gatacggttc aatactgcgg
    13201 atgcagtcac tccacaattc tctcatcatt cacgtattaa gtgaaatcct ggttccactt
    13261 ttccttaagg attcttaggc taaaaacaag caaagatcac accacaaata tgtcagccct
    13321 tttcaattaa atctctatga aaacagcata gcataatttc agcccccagg aaaaaaggtc
    13381 ttacatctca tgcagggcaa tcaatgcatc aaccgcaaca ggcttaccgt catcatcaag
    13441 tataaccggg ttgatgtcaa cttccattat tcttttctct tcaaggccaa tcttgcccac
    13501 aaccatgatc atgcgggcaa gttcatccat atcggcaccg ggcataccac ggaaggcatt
    13561 cagaatttta tgggctctaa aatctcgcat catacggagt gcctctgcct tttcgacagg
    13621 tgcaatccga acacagatat cccggaagat ttctgtaaaa attccgccca aaccaaaggc
    13681 aacacaagga ccaaactgaa ggtcacgttt gagacctatc atcagttccc tctctccact
    13741 caccatctcc tgaaccagca cgggaaatcc ctctgcctca ttcttatctg ttatttttct
    13801 gtaggcggcc atggcttccc tctggtttct aatatccacg tgtacaagac cacgttctgt
    13861 cttgtggctt atatgggggc tactaacctt tactgcaagc ggaaacccga tctcaataag
    13921 ggcatcttta aaacttttcc ggttatgagc ctccctttcc ttcgtaactg gaatgccata
    13981 ggctcccagg acccgttttg actcatgttc ggaaagaata tgtcttcctt ccttgagagc
    14041 attggcaatg atatccgttt gttctgtgaa atgcgcatca gattttttcc ttttaccggc
    14101 gtctttcata tacatatact cccaccagca cgaaactttg catattccca cagttttttt
    14161 aaaaccttgg ctgccctcgc agcatcagga tatacagaaa taccgctgcc aagcatcaag
    14221 gggaagtctt gtgccacctc ctgcgttgtt ccacccatga gtgtcaccac aagaggcttt
    14281 ttattttcaa cattccgtgt tgcatcgaga acaatttccc tgagttgctc tccacctatt
    14341 acggttataa gaagcaacat gtcgatgttt ttgtctcgcg ccactgcatc aatggcatct
    14401 ttatgtatga ggggattaac gagtgatgca aggctgagat cgatgggatt attaacgctg
    14461 cctccaacag gcggaattat cctgcgaagc ttttccactg tttctttgga aaaatgggga
    14521 agctcaagac caagttcaag acaaatgtca gtagtgccca ctgctgtgct cccgggtccg
    14581 ctgatgatac caacgcgctt tcccttgggt aagggctggt tgtaaaaagc atagagataa
    14641 tcaaacacct cttcgaaact ccttgcatca actattcccg cttgcctcaa tgcaccttcc
    14701 caaacagcac gggaaccggc aatggcgccc gtatgaaaga ctgctgctct cgcccctgcc
    14761 tcggtaagac cacctttcca gaggattatg ggcttctttt ttgatatttc cctcgatagt
    14821 tcatagaatc gcctgccatc ctttacccct tctatgtagc cggcaattag ctctgtttca
    14881 gggtcttccc ccaggtattc cagaaaatcc acagcattga gatctgcctc attgccgcag
    14941 cttattgctt tactgaactt tatattgttg gcgcaggctg tctgagtaat aaaatcagcg
    15001 aagaatccac tttgggacac aaccccaacg atgccggaat ctttacctgc ctgacacagg
    15061 aagggtaact ttgacgaagg gcagtatatg ccaacacagt tgggtccaat gatacgggcc
    15121 cctcctttcc gggcaatatg aaccatctcc tgctgtactt tccttccctg ttctcctgcc
    15181 tcaccaaatc cagaagtagt gactacgatg gtttttacct tttttgtcac acaatctttt
    15241 acggctgcca gagcagcgtc tgtgggtgta agtatgattg ccaaatccac aggacctggg
    15301 acatccttta tgttgtgaaa aactctcagc cccataattt cgctttccct gggattgaca
    15361 gggtagaggg gcttgaatcc catctccaaa aaacggtcca tgagtacacg ggctatcttc
    15421 ccctgcttgg cggatgcacc gagtaccgca accgacctgg gatgaaagat cgggtccaat
    15481 tcatcgaaga tagtcttccc tgacatacct ttgctcataa cttacagtcc cattatttgt
    15541 caatgactaa atagctctga ttaattgcgg aacaacacac tatacctgca tctctgatgc
    15601 aacacaatat catgcagtca tatacgtgta agccactaac tctaaaggaa ttgcaggatc
    15661 acccgaataa tatagcaaag taaaagacta tgcccccaat ggtaataacg aagtattgca
    15721 gtttcaatga atagggatat cgtcatgaag gctatatatt gtatgtattg tttgctcttt
    15781 gcaatcacaa tcatctctcc tgcagctact gcccagggag ccgagtggtt ctccataggt
    15841 gaggacagag caggtaatga attatttttt gatcttgagt ccctggtgca gcattcagga
    15901 gtaattaaag catggatgaa agcaatatac tccagagaag gcaggaaaga aagaatcaag
    15961 gaaagagtca tgaagaaggc ttcagtggag cgttatgaca ggctgagtta tgcgctggag
    16021 ctacaggaaa ttaactgcgg tagaagagaa tttcgtgtct tggcatatgc tgattactcc
    16081 tctgacggga gaatccttca cagttctatc atcgatcatc accctgcaga agggtgggaa
    16141 cccatagctc ccgattcaat gggagaagta atagacaggc ttgtatgtcc atcatccccg
    16201 ttacagaaaa agaagctgcc aggcattata gagagggggg gcttcattaa gatcaacggt
    16261 agcgatttaa tatcaggggt aaaatcacca ttgtagaaaa gatcaaacca aaaagtccgg
    16321 attcctccgg gtctttttgt tgttggggtc acagtaatgc tgggcaggtg cgtatcgaat
    16381 gccaaggagg gggcagttcc aacaagggct atggaagtaa ggagcatctt tatggtagga
    16441 tgaagccagc gtcatcatgg gtagagcatc acctggagga ttgacatgta tattacatca
    16501 tttgcacatc gtgaagagct ttttcaaatg gttcaacgat ggttctgcaa tcaacttgaa
    16561 ccaactgacg gtcatagggt gacaaaaatc ctgatttgtg atggttttat tctcggagaa
    16621 acccttaatg atcttgtacg gctgcttatc tccacttttt ctgatgaaac ctatcgaaca
    16681 cagcggcttc acttcaaagg tgagctcaga gatatcatct gccagagctc tcacgagcgc
    16741 aatgaccgca ccacagagct catagaaagc tataaaaaca atcctgattt ttatttcaca
    16801 gaggctccaa tcgatggtag cgcttatctg gacacaatgg cccgattgtt agccgtttac
    16861 agaattaaac ggcccaggag gatagcagaa aaagcaaatc gttatattgc aaatcacatc
    16921 ttcagaatgg ttcagaacag ggctcgccag atggcggaag aacgggccca gcagattggg
    16981 gtgtcattgg aacacctttt aaccacagaa gaagatatgg aacgtgagtt tacgctcgct
    17041 gaggagatga ttgcaggggc tttcaggcag ggttacgcga gatttgagaa acctgcagtt
    17101 acaatcaatg acgttggggg cataaaatac attgccaatg aggaatcatt acatgcgatt
    17161 gaaaattttt tccttaccca ccctgacttc aaagtttttg aacgatcaga acacactggg
    17221 aactatcgtg cgaagagcct gatcctcgaa gtcccatggg acaaagaaaa ggtgtgtaga
    17281 agctatcttg aaactcatgc gtggaagaaa tacctgaaac gcggcatttc agaagctgaa
    17341 cttcaaaaag gcctggagca tttagtagaa gatgcagagc caagaatctg cattgaagtg
    17401 acgttaacca cctttgatga tctcgttgaa tctgagtttg gacgaggtat ccacgaggaa
    17461 agaatcataa cccaacgcgg tcagccagtt tacgtggggc acattcccct gaatgtggga
    17521 tttctcgttg aatatctttt tgccgttgct gtcagtccaa gaatccatat tgaccggctt
    17581 cccatcaagc tgtggggacg ttacctgccg gacacaataa gctatcatat ccggcagctc
    17641 tattacctgc cggagcatga tgcgctttat taatgtaact ggacagaacc aggctgatct
    17701 gatccctaat ccttctcctg gataggctta tcccctgtat gaatgtaatc gaaacgcaga
    17761 tagttctgaa gagacatcgt gaacagggga tgattcatgc agattttttc aagcctttcc
    17821 tttacaatga ctgctactga ttacggtttt taacccacct cccttttttg gttgctcaag
    17881 tttcctctct tctcgaattg tattccaggg gatagggctt gaaagatatg tccgtacagt
    17941 gaagatgaat gtttctatca tcctgaccgt ttaaacatcc gggtaagggt tgcttccgca
    18001 tcaggaaatc tctgtacatc aaatacctga gtgaggatat ctttgtttta tttttctccc
    18061 tcttgccggg ttggtcatat ggtagacaat tgattcaact gcaaaggaaa ttttgtggct
    18121 taatatcaat tttcagggcc tgaattagct tctaaatgcc atactggtgc t
//
LOCUS       DAOOMC010000054        18084 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdus sp. isolate AMR_MDS_2685
            NODE_3210_length_18084_cov_6.029064, whole genome shotgun sequence.
ACCESSION   DAOOMC010000054 DAOOMC010000000
VERSION     DAOOMC010000054.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864760
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdus sp. (wastewater metagenome)
  ORGANISM  Syntrophorhabdus sp.
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae; Syntrophorhabdus.
REFERENCE   1  (bases 1 to 18084)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18084)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,729
            Genes (coding)                    :: 2,708
            CDSs (with protein)               :: 2,708
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 7 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18084
                     /organism="Syntrophorhabdus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3210_length_18084_cov_6.029064"
                     /isolate="AMR_MDS_2685"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2699756"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..210
                     /locus_tag="PK125_07005"
     CDS             <1..210
                     /locus_tag="PK125_07005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB37892.1"
                     /translation="KKLDIGSDASGHRFRQRVPKVARRVVKLCTGSPSKVCFTVFFFV
                     AEGDTSAGLTGSLVSSRPGSSSVIV"
     gene            complement(366..1238)
                     /locus_tag="PK125_07010"
     CDS             complement(366..1238)
                     /locus_tag="PK125_07010"
                     /inference="COORDINATES: protein motif:HMM:NF014046.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ARMT1-like domain-containing protein"
                     /protein_id="HPB37893.1"
                     /translation="MFMQADYCIPCMLKMSVEIIRGVTKDEKQVRKFMTDILKIKALR
                     WEKVAITPPEVARDVWFRMQQFSSIEDPLRATKAEQNKKALKIYPFIKEILHESSDPI
                     LEALKFAIAGNSIDAMLDAKRGMDSKIIKLLSSINVKDVEVLKRRFKKAKKIVYFTDN
                     CGEIVFDRLLIERIKEVYNSQVTAVTRTVPILNDAIMEEALSIGLGEVTMVIENGIQE
                     PLPGTILSKVSPEIKAAVEESDLIISKGGGNYETLTEEESLKGRITYLFQAKCYPYCR
                     IHNVPLGALIVYNN"
     gene            complement(1946..2131)
                     /locus_tag="PK125_07015"
     CDS             complement(1946..2131)
                     /locus_tag="PK125_07015"
                     /inference="COORDINATES: protein motif:HMM:NF013481.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016625 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, iron-sulfur
                     protein as acceptor [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase C-terminal
                     domain-containing protein"
                     /protein_id="HPB37894.1"
                     /translation="MFKEPLQNAGLAAGHVVAKMDTILDEYYAYMGYDANGVPTAAKL
                     KELGLTDAANEMEKFRK"
     gene            complement(2376..3401)
                     /locus_tag="PK125_07020"
     CDS             complement(2376..3401)
                     /locus_tag="PK125_07020"
                     /inference="COORDINATES: protein motif:HMM:NF013491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HPB37895.1"
                     /translation="MEKWIFWFDDIGAEANDFVGKKCANLGEMTKLGMRVPYGFAISV
                     NGFEKFMNLTGLSQKVKECVESERDRLQQIEVCQNLSRTIQGLVEATPMPDAMQSELA
                     AHYRELCAKAGREIIPVAVRSSGVISMPGQMDTYLNVIGEDAVVEHVRKVWASSYTTR
                     AITCRIEQDRPVEWAPIGVAVMMLVHAKSAGVVLTVLPTSGDTSKVVIEGNWGLGESV
                     VSGEITPDSFVVEKDDLRITSRNITHKKGMVQRGEHGTVYVPVPDELQDKPCLDDDEI
                     LEITRTALRVEQHFGVPQDMEWVVDRYLPFPENIFWVQARPARFTKANATEDIDYIID
                     LMAQIFK"
     gene            complement(3493..3672)
                     /locus_tag="PK125_07025"
     CDS             complement(3493..3672)
                     /locus_tag="PK125_07025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB37896.1"
                     /translation="MAKEYKIDEMDAKIQEIKKAVEEIEKLGGDIEAVKKNLVRLRAS
                     TKMLELNISDVKLVM"
     gene            complement(3690..5018)
                     /locus_tag="PK125_07030"
     CDS             complement(3690..5018)
                     /locus_tag="PK125_07030"
                     /inference="COORDINATES: protein motif:HMM:NF025888.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB37897.1"
                     /translation="MAKNGSIYWNPIMETMPREKLRELQVKKFKRVFEWAYKNSPFYK
                     KLYKDAGMEPGDIKTLEDIKKVPKTDKGMLREVQNRPPFPYGDMLAVPLQDVTEFRQT
                     SGTTGTPVYQADTWQDWEWWAESWAYILYAQGYRDTDRMFLPFGYNIFVAFWACHYAA
                     EKLGCEVVPGGVLDTEARIMKMQELKCTAFGATPTYVLGMADTARKIGIDPRSIGIQR
                     ITCAGEPGASIPTTKQRIEEAWGAKVYDHIGATEIGAWSYMCTAQKGLHINEAFFLVE
                     IEDTETGEIIEEPMKNGKMIITAFDRQAKPCIRFDSKDVIRWADYECECGRTFRIIDG
                     GVVGRADDITKVKGVLLAPTAIEEVVRSFNELSDEYEVIVTKKGDIDDIMLKIEIKPG
                     FEKQEADILNRLKDQLRVKTNLGYKIEIHPYESLPRYALKAKRFKDLRPH"
     gene            complement(5083..5739)
                     /locus_tag="PK125_07035"
     CDS             complement(5083..5739)
                     /locus_tag="PK125_07035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB37898.1"
                     /translation="MFLFVQQWEIIRGKGAEYTDFVLRRHLPVMTKVGLNMIGGFHVI
                     VGSGPRISAVSLAHDFLNLQKAIESEEFIEVTRELHEFVYNYNSAVLKRTGRVNMEGY
                     GIEVGTWRFNQYYKLIYNTEKEYTRFLQNDYIPTLTKLGIRIKAEWQVILGAGLRILL
                     EGVTTNLSDVAQAIMSDEFRSLRRQLLSNFAGNYSSRILVPTGRVEIAYLLGEMTKAL
                     "
     gene            complement(5825..7510)
                     /locus_tag="PK125_07040"
     CDS             complement(5825..7510)
                     /locus_tag="PK125_07040"
                     /inference="COORDINATES: protein motif:HMM:NF012609.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-utilizing enzyme"
                     /protein_id="HPB37899.1"
                     /translation="MKIYDCVPGLEFDEKDDLEQSPAWFLDGTHSIPPWTPMFGWFWV
                     NFCRHGMQYGAETLYLPRSKGWDWRFKNGGGYLTILLVDSEDEMKEREAKFRHAIRPF
                     LENYDGLWDGFVNEMLGRYGKIKAVNGDTATNIELLANFDYAVETCRRMWEIHMYMMY
                     GTYMPYVLFEQLCKHLLNIDDTHPDFHRLMSGFDNESFRVDRGLWEYAQKLKDMGLAD
                     VVVGVPTKEWEGKLVASEKGQEFLKDFKIFLDTKAGWRMERMAEINVPSWSEEPSQAF
                     DTMEIYLKQGKEFDLDAKRLMLEKDRKVAEKEILERISPEQRGWFSILLKIAQNSSRF
                     SEEHDHYLDLYCHSVVRKTCIDIGKRFAAGGAIAETDDIFFLIPDEVHRAGINPGKFN
                     MKNIVARRKNEWSKWNETGNPPIILKKDFGIAEAMEFMIKSMDPIALKVVVGKLPEAR
                     PDLKADLYGTCGSPGVAEGIARTVFKEEDLAAIQEGDILISVSTSPAWTPIFGMIRGV
                     VVDRGGSLSHAAIVGREYGIPVVMNTFEGTAKIKSGQRIRIDANLGTVFILDK"
     gene            complement(7631..7951)
                     /locus_tag="PK125_07045"
     CDS             complement(7631..7951)
                     /locus_tag="PK125_07045"
                     /inference="COORDINATES: protein motif:HMM:NF015925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HPB37900.1"
                     /translation="MYVRMTFFKVKPGKMDNLRNTYVNHVIPAHKGHKGIRFVHLFES
                     MESVNEGLSLTAWDTKGDLEAYEKSGDYQRILSMFTEYFDGTPTLKSYEVTASSEPMI
                     LRIF"
     gene            complement(7973..8728)
                     /locus_tag="PK125_07050"
     CDS             complement(7973..8728)
                     /locus_tag="PK125_07050"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase family protein"
                     /protein_id="HPB37901.1"
                     /translation="MGRLDGKVAIITGAGKGLGAAFAVRFAAEGAKLCLVTRKDMEGL
                     KKVAEQVTAKGAECMWFQADVTKLEDVNRMADETVKKFGKIDILVNNAAYYFGVERRP
                     FNKISQEEWDMMMAINVKGPWLCTQAVYPHMKAQGKGKIVNLTSEVFFTGSNGFVHYV
                     ASKGGVVGLTRALAAELGPDNITVNAVAPGFTDTEASRTIADVKKYDTSKTPLRRLGV
                     AEDIVGAALFLASDDADFITGEIILVDGGRAMH"
     gene            complement(8812..10527)
                     /locus_tag="PK125_07055"
     CDS             complement(8812..10527)
                     /locus_tag="PK125_07055"
                     /inference="COORDINATES: protein motif:HMM:NF012609.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-utilizing enzyme"
                     /protein_id="HPB37902.1"
                     /translation="MGQWMFKRHVREFWPERDYKKYPVWFVDFSHSYPAWTPLFEWTW
                     IFNLLHGHIYGCNEAWIPETRSSDWREIDGMAVCTATPVPNKEEVEFRRKKFYEFIKG
                     DFEPNYDKHWERSKNDMLTRYSTLKDFDYENATYYDLYKQFNYAIETHKRMWEDHFWF
                     MYALYGAYWYFEDICKDHVGIKDTSTLWHKLIRGYDNMLFRQDKAMWELRNKAIEYGI
                     DNIIANNPSVKVIEELNKTPQGKKWVDEDLNNFMHGLEYGWRMIRMMEFVEPTWWEAP
                     ENAIIHVKQYLMIDREIKPFPLDAIREKLIRERDEAQKEIIEKAEASGYKDMDWFKCL
                     MRLAQRTSSFSESHDFLYEHRCFSAFRFVMLKIGDRLAKVGTFNQADDIFFFIPEELQ
                     TMIAMPENYDSGFIAKERRMEWLRSKEFLSRPPIMPRDPKMQPHEAGAFMAAAKDPII
                     AKITIGEFVAPDPSTGATCFGNPGSPGVAEGPVCVVITATDLKKLKPGDIMVCPSSQS
                     AYTPVFPMLKGLVCNGGGSLSHGPIVGREWDIPVVANCITGTQLLKDGDMVRVDGFKG
                     LVYKL"
     gene            complement(10682..11581)
                     /locus_tag="PK125_07060"
     CDS             complement(10682..11581)
                     /locus_tag="PK125_07060"
                     /inference="COORDINATES: protein motif:HMM:NF014046.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ARMT1-like domain-containing protein"
                     /protein_id="HPB37903.1"
                     /translation="MAPLEKGGIVKPDIKCGLCLLEWVYGRAVSQNRNKDIPQLFKNI
                     TKLLAHNMDSSTNLGAICNDAVDLIYEYVTPRSPFWDKLKQKTNEYVKSLLPKGEEFI
                     KSAKTPKAMLERAFYLAATGNVAPMGAPSGAFTFPEAVDMMEGKGSPPVLMGNVYETV
                     VNSKNVLYVTDNAGEIGFDSLLISQLRAMNLWVTLVVKDPAYFEDATMDDAVFYGLDS
                     IANKVVTANKVFVPGNEKTPVNEAFRKSDLIISKGTGNYDALRGETDGKAAIFLLKVK
                     CEPIAKDTGINEGKFVVRLDTGI"
     gene            complement(11651..13336)
                     /locus_tag="PK125_07065"
     CDS             complement(11651..13336)
                     /locus_tag="PK125_07065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HPB37904.1"
                     /translation="MPDRKYDAVIVGGGHHATIIACYLARAGLKTAMFERWHEMGGGA
                     CGEELPVPGYIQNVCAHFTRFYTNPAYTDFELRDHGLVYVFPENNEAWIYPDGSYIFG
                     KTMFPVIDPFTGKSSFSQEAADYTIKSVGEINKDDGDTVAQIIESYKGAWKEAFGKYR
                     YSGPGEWGDGPDPLEALFNHPKYPLDPKIALGTVGEIATTMFKSPEMQTFYMRAIQTS
                     NGLFPQDRPGPYWHIHALGLILSMEPAAIVQGGTHSITHALLRAFERYGGEFFVLNEV
                     NKVLVDNKKAYGIELTNGDKILADIVVSDLSIELTMDKSGKDNWPAELWAKGQKLKDK
                     PDDLYNTSAYERTQLLWGNIALMDAPKYPQNPELGMVPRLYFGKADPEYFLSGRYQKE
                     RWDLGIANQMYLLVAPDVQWDPTRAPAGRFAALVEEFTCPWRQFSEKQWLKIKKDIEF
                     RMVEEWGKYAPNVNMDNFISAWIATPDDVVNRNPCMPQGGWGALDANYERLGRLRPFP
                     EISGYRMPIKNYYLCSSAAHSAHGIGRGSSYACYKVIAQDLGLKYKPWEERGW"
     gene            complement(13377..13784)
                     /locus_tag="PK125_07070"
     CDS             complement(13377..13784)
                     /locus_tag="PK125_07070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB37905.1"
                     /translation="MFVRSTGLGRTLLTGRIGKVVITSLVPATLEEAPNGSKEPKRML
                     MEMEIQHPVHWTVRAFLDPPDLRSLVKKIITNPGLMMTGLKFLFMKGPRYDVPTSHTA
                     APQAASTGPAAPPKAASPAAGTPPKGPPPIPSR"
     gene            complement(13795..15435)
                     /locus_tag="PK125_07075"
     CDS             complement(13795..15435)
                     /locus_tag="PK125_07075"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HPB37906.1"
                     /translation="MRELEYDGIIIGAGPNGLTTAGYLTKAGLKIAILERRYEIGGGL
                     ATENLLLPGMLVDSHAIYHMLVEYAPPLRDFEFETRYDLQWIYPDLQMVMPFQDGSYL
                     ALYKDPEKTAQSIRQFSEKDAESFLNFAKISGEAMDLFLAPATYVNPMPSLEQVAKLE
                     THPVTKWDDELTGYSPKQIVESWFENDKVRALMLYCATMWGLDYDLEGLGYLLPLMIN
                     RAWHFRLCHMGSHHIAHLFGKYISEEGGRVISGCVIKRIIIEDNEAKGVELEDGTILK
                     AKHFVCSALNPHQTFYDYVGKQYLPPDMITRLDQWTYSDLSFFTVHLALTEKPHFKIF
                     DKHPELENSLIYLIGYESEQDLINHLDASKRGELHDGGFNCCFPSIHDPIRVHRHKDS
                     FVKHIGLLTMECAPYNLKDGGALAWNRMRRPYAERCKKILEKYAPNMNKDTVVWDYIG
                     TPLDTENKFPDMKQGCFKQGAYLPLQMGFMRPNEFCCQHDTPIKKLYVGGSSTHSGGM
                     IVYGPGFCAAEKIAEDLGIKKWWPEPRGVKEAKDLGLF"
     gene            15608..16546
                     /locus_tag="PK125_07080"
     CDS             15608..16546
                     /locus_tag="PK125_07080"
                     /inference="COORDINATES: protein motif:HMM:NF015431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HPB37907.1"
                     /translation="MNLNQLRGFYTVLKTGSFSRAAEELCVTEPAVFIQVRSLERHLG
                     FTLLDKVGNELHPTAAGRILYEYGEKIFTLVDEAVNAVQGLQDLKHGHLRVGVTQALA
                     QYLMPVLIPGFQERYPHITVSMDGGSSRGLVEGILQQRYELAVVARVPYPDKITSIPY
                     TRDNILLIISPHHALAKKEQVSLAELAKEPVILTGAKSAVKFSVWKGFEKRGLHPAAI
                     IEAGNIEFIKQLVEKGKGYAFLASVCVLEEIRKGVLATVPIEDESFTVDIDVIHLRGR
                     TLSPAASTFLAFMQEHRKSDSLRILTDEITKKTTTS"
     gene            complement(16543..17784)
                     /locus_tag="PK125_07085"
     CDS             complement(16543..17784)
                     /locus_tag="PK125_07085"
                     /inference="COORDINATES: protein motif:HMM:NF018498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tm-1-like ATP-binding domain-containing protein"
                     /protein_id="HPB37908.1"
                     /translation="MPTVLLLTSFDTREKEVMLLKGFIQEQGCDVITLDISTGTCREG
                     VADFSCRHATAELAIDFEVPSTTSNTYLTMDYVILAGLSIARKLYNEGKIDGLAGIGG
                     TSNVTIVSHIMKEFPFGIPKLILTSSAAIPAYAAKFFADTDISVFHSCVEINTLNVFV
                     RDILDRFAGMVAGTLKASKKKLGKGPPAIAISEFKFSETCTARVRELLEEKGYQAVPF
                     SATGPGERIMERMVEKGYLRGVIDVVPAGLSEAILGGNRASGLDRLDKELASGFPVIL
                     TPCGFDMLSCGPYDRKDADPLWKQKRLAQRKLFIPDRFRVQARTSRKEVELVARVFAE
                     KLNKSKSRVFVFIPLLGFSSLSKAGGPLFDPQTDRAFVRIFANSIKGDKVEIIALNNT
                     IDDPEFADAMVEKFLQLFQGI"
BASE COUNT         4589 a         4600 c         3576 g         5319 t
ORIGIN      
        1 aaaaaacttg acattgggtc tgatgcaagc gggcatcgat tccggcaacg ggttcccaag
       61 gtggcaagga gggtagtgaa gctttgtacc ggaagcccgt caaaggtatg cttcacggtc
      121 ttcttcttcg tagcggaagg tgatacttct gcaggtctta ccgggtctct cgtctcctct
      181 aggccgggaa gctcttctgt gattgtttag ggatttcaag aaagtaatca gcaaaggttg
      241 gagtggtaaa ttcctgactc acaggtacct cctcttgtgt tacacaaaat ggagcatatt
      301 tgtctaatgt cataaaaagc aggtcacgat aaataacgtt tatcaaatat atattttcga
      361 catctttagt tgttgtacac gatgagtgcc ccgagaggga cgttatgtat tctgcagtaa
      421 ggataacact ttgcctggaa aaggtatgtt attctcccct tcagactttc ttcctcggta
      481 agggtttcgt aatttccacc tccttttgag atgatgagat cagactcttc aactgccgct
      541 tttatttcag gtgatacttt agagagaata gttccaggta atggctcctg aataccattt
      601 tctattacca ttgtaacttc ccccaatcca atagacaggg cctcttccat tattgcatcg
      661 ttgagaattg gaactgttct tgtcaccgcg gtcacctgac tattgtatac ttcctttatc
      721 ctttcaatga ggagcctgtc aaagactatt tcaccgcagt tatcagtgaa ataaacaatc
      781 ttttttgcct tcttgaaccg tctcttcaaa acttctacat ctttaacgtt gatgcttgat
      841 aaaagcttaa tgatcttgct atccattcct cgtttagcgt ccagcattgc atcgatggaa
      901 ttgccagcaa tggcaaattt gagggcctct aagataggat cagaactttc atgtagtatt
      961 tccttgataa aaggataaat cttaagggcc ttcttgttct gctctgcctt ggttgccctc
     1021 aaggggtctt caattgagga aaactgttgc attcgaaacc agacatcgcg agcaacttca
     1081 ggtggagtta tagcaacctt ttcccagcgg agagccttta tcttcaggat atcagtcatg
     1141 aacttccgca cttgcttctc atcctttgta acgcctcgta taatctcaac ggacattttc
     1201 agcatacatg gtatgcagta atcggcttgc ataaacataa attttaagcc ttcctccttt
     1261 gaggataatc actctcttgt attctttctt tcacatgcaa gtccatgtca attctgtccc
     1321 tgactgaaca aagcgattcc agcaattgtt ttaaggtacc aatctccatc aaggagcggg
     1381 gaaaaaagaa tggtatctcc ctcatggaac atgaactcat ccatcttatc aggcaatatg
     1441 tattttcccc aattcacaat aatctggagg ttattttcat aacaactatt tgtggaaaaa
     1501 accttttcag tcttcccacc ttatattttc atgatttccc gaacaaggtc tgatacagct
     1561 tgttcccttc aaatcgaagg taatcatgtt atctccaaat gctttccgtt caccttcaac
     1621 ttcaagatat tttacattaa tcttaaatta ttctctgtta ttatatcctg ccagtccctt
     1681 atgcgtcaat gacactggta gttcacatat accctattta ggatattgca taccgaataa
     1741 cgtaagcaaa catatacttt tttcttgctg ttttttttgg ctaacgaaca cagtatgcaa
     1801 ccaaccaact tcaaagaatt gtagaattac attttatcga ggattatcaa tatactatat
     1861 gcggacgaca atacacagcc ttccttaaat gcgaatcaaa aaaaaggggg gggtcgaatc
     1921 ccctattatc atacatgaac agttcctact ttcggaactt ctccatttca tttgccgcat
     1981 cggtcaatcc caactctttc agctttgctg cagtcggaac accgtttgcg tcgtacccca
     2041 tgtaagcgta gtattcatca agaatggtgt ccatcttggc tactacatga ccggcggcca
     2101 gtcctgcatt ctgtaagggc tctttaaaca tccgctcttg gtgactccta aaattccagg
     2161 aacaaacagg aggatgttgg ccgggctgaa cggatcagta cccggcaaag gtttttgtac
     2221 aaatagttga tgccgaagtc tttgccgccg agaaaatctc tgataacgtt gctctcagtt
     2281 ggtatctctg ctgcctctcc tttgggtaaa ctgagtctga gtagtctgtt tcgatatact
     2341 gacatccctt ttcttctgcc cttcttacaa caaaattatt tgaaaatttg tgccataaga
     2401 tcaatgatat agtcgatatc ctcagtagcg tttgcctttg tgaatctggc aggtctggcc
     2461 tgcacccaga agatattttc agggaatggg agatacctat ccacaaccca ttccatatct
     2521 tgtggaacac cgaagtgctg ctccacacga agtgccgttc gcgtgatctc aaggatttca
     2581 tcatcatcaa gacatggttt atcctgcaat tcatctggca ccggaacata tacggtacca
     2641 tgttctccgc gctgtaccat tccttttttg tgggtaatgt tgcgacttgt aatccttaaa
     2701 tcatcctttt ctacaacaaa cgaatcgggg gtaatctcac cacttacaac gctttctccc
     2761 aacccccagt ttccctcgat cacaaccttc gatgtatcgc cggatgttgg caagaccgtg
     2821 agaacaaccc ctgctgactt cgcatgaaca agcatcatca cagcaacgcc aataggtgcc
     2881 cactcaactg gtctatcctg ttcaattctg catgttatgg cccgtgtcgt gtacgaactt
     2941 gcccatactt ttctcacgtg ttcaacaaca gcatcttcac ctattacgtt caggtatgta
     3001 tccatctgac caggcatgct aataacgcca cttgaacgaa ctgcaacagg aatgatttcc
     3061 ctgcctgcct ttgcgcagag ctctctgtaa tgagcagcaa gttcgctttg catagcatca
     3121 ggcattggcg ttgcttcgac aagaccctgg attgtacggc ttaaattttg gcaaacctct
     3181 atctgctgga gtctatccct ttcactctca acacattctt ttaccttctg ggaaaggccg
     3241 gtgaggttca taaatttctc aaaaccattt accgagattg caaatccata gggaaccctc
     3301 atgccaagct tggtcatctc gccaagattt gcacattttt tcccgacaaa atcatttgcc
     3361 tcagcaccaa tatcatcaaa ccaaaatatc cacttctcca tactcgcgta acccctggcc
     3421 cccggtgcct cccctgcaaa ccttatttta caaaaataaa ttttgcagga gaggcggggg
     3481 taaagacatc ttttacatga ccagttttac atcagaaatg ttcaattcaa gcatcttagt
     3541 gcttgcacgc agtctcacaa ggtttttttt gactgcctcg atatctcctc caagcttttc
     3601 tatctcttcg actgcctttt ttatttcttg gatctttgca tccatttcat caattttata
     3661 ttcttttgcc atgatcgtgc cctccttgct tagtgtggtc tcagatcttt gaatctcttt
     3721 gctttcaacg cgtaccgtgg aagactctca tatggatgaa tctctatctt gtatccaagg
     3781 ttggttttaa ctctgagctg atctttcagg cggttcagaa tatcggcttc ctgcttttca
     3841 aaaccaggct ttatctcaat ctttagcata atatcatcaa tgtcaccctt ctttgtgact
     3901 atgacctcat actcatcgct caattcgtta aagctcctta ccacttcctc aattgctgtt
     3961 ggggccaaaa gcacaccctt aaccttagta atgtcatcgg ctctgcccac aaccccgcca
     4021 tcaatgattc tgaaggtacg accacactcg cattcataat ctgcccagcg gataacatct
     4081 ttcgagtcaa acctaataca tggttttgcc tgccggtcaa aggcagtgat aatcatctta
     4141 ccgtttttca tgggttcttc gataatctcg cccgtttctg tatcttcaat ctccacaagg
     4201 aagaaggctt cgttgatatg gaggcctttc tgggcggtgc acatatagct ccaggcccct
     4261 atctcggttg cgccgatatg atcataaacc tttgcacccc atgcttcttc aatgcgctgt
     4321 tttgttgtgg gaatgctcgc gcccggctca cctgcacagg ttattctctg tataccaatt
     4381 gaccttgggt caatgccaat ttttcgagca gtatcagcca taccaagaac ataggtggga
     4441 gtagctccaa aggctgtgca cttgagttcc tgcatcttca ttattcgtgc ctccgtatca
     4501 aggacaccac ccggcacaac ctcacaccct aatttttctg ctgcataatg gcaagcccag
     4561 aaggcaacaa agatattgta gccaaaaggg agaaacatac gatctgtatc tctgtaaccc
     4621 tgtgcataaa ggatgtatgc ccagctttct gcccaccact cccagtcctg ccaggtatcg
     4681 gcctgatata ccggtgttcc agtagtaccg cttgtctgtc tgaattctgt aacatcctgc
     4741 aaaggaactg caagcatatc accataagga aaaggaggcc tgttctggac ctctctcagc
     4801 atgcccttat ctgtcttggg caccttcttg atatcttcaa gggtctttat atcccccggt
     4861 tccataccag catccttata aagcttcttg taaaatggtg agtttttata tgcccattca
     4921 aaaacccgct tgaacttttt cacctgaagc tcacggagtt tctcccgggg cattgtctcc
     4981 atgatcggat tccagtaaat gcttccattc ttggccatcg acatcctcct ttttaaattt
     5041 ttcgtgaagt cttacaaacc ttctcgcgta caacaattcc ctttacaggg ctttagtcat
     5101 ttcccctaaa agatacgcaa tctcgaccct tccagtaggg acaaggattc tgctgctgta
     5161 atttcctgca aaatttgaaa gcagttgccg tcgtaaagac ctaaactcat ctgacatgat
     5221 tgcctgagca acatcagaaa ggtttgtggt aactccttca agaaggatgc ggagacctgc
     5281 accaagtatg acctgccatt ctgccttaat tcgtataccc agttttgtca gcgtaggaat
     5341 ataatcgttt tgaagaaatc gcgtgtactc tttctcagtg ttgtaaatca acttgtagta
     5401 ttgattaaac cgccacgtgc cgacctcaat gccatatccc tccatattaa ctcttccagt
     5461 acgcttgagg acagcactat tataattata gacaaactca tgaagctctc ttgttacctc
     5521 aataaattct tcactttcaa tcgctttctg gaggttcaaa aagtcatgtg ccaaggatac
     5581 tgcactaatt ctcgggcctg atcctacaat aacatgaaat ccgccaatca tgttgaggcc
     5641 gactttcgtc attacaggca agtgtcttcg taacacaaaa tcggtgtatt cggcaccttt
     5701 ccctctgatg atttcccact gctggacaaa taaaaacatg attactcacc tctctgtatt
     5761 agatagtacc gatttccgat ttgtgatcct caccactaac cgttctcgac tcctgcctta
     5821 tttttcactt atccagtata aagaccgttc ctaaattcgc gtcaatccta atccgttgtc
     5881 ctgattttat ctttgctgtg ccttcaaatg tgttcatgac aacaggaatg ccgtattctc
     5941 tccctacaat tgctgcatgg gaaaggcttc cacccctgtc cacaacaaca ccgcgtatca
     6001 tcccgaagat tggtgtccat gcaggagagg tgctaaccga tatgaggatg tccccttctt
     6061 ggatagctgc aaggtcttct tccttgaaca cagtccttgc aatgccttca gcaactccgg
     6121 gagaaccaca agtaccatag agatcagcct tcaaatcagg ccgtgcctcc ggaagcttac
     6181 ccacgacaac cttgagggca atagggtcca tagactttat cataaactcc atagcttcag
     6241 caataccaaa atcctttttg agaataatcg gaggattacc ggtctcattc cacttgctcc
     6301 attcattctt acgacgtgcc acaatatttt tcatattgaa tttcccggga ttgataccag
     6361 ctctatggac ttcatccgga ataaggaaga atatgtcgtc agtttcagct atggctcctc
     6421 ccgccgcaaa cctttttcct atgtcaatac atgtttttct caccacagaa tggcaataga
     6481 gatcaaggta atgatcatgt tcttcgctga aacgactcga attctgagca attttaagaa
     6541 gtatggaaaa ccatcctctc tgctctggag atatccgctc aagaatttcc ttctctgcaa
     6601 cctttcggtc tttttctaac attagacgtt ttgcatcaag atcgaattcc tttccctgct
     6661 tcaggtaaat ttccattgta tcaaaggctt gggatggttc ttcagaccag gacggcacat
     6721 tgatctcagc cattcgctcc attcgccatc cagcctttgt atcaagaaaa attttaaaat
     6781 ccttaaggaa ttcctgccct ttttcagatg ctacgagctt gccttcccat tcttttgttg
     6841 gaactcccac aactacatca gcaagtccca tatcttttag tttctgggca tattcccata
     6901 aaccacggtc taccctgaag ctttcattat cgaatccgct catgagcctg tggaaatcag
     6961 gatgggtatc gtctatgttg agtaagtgtt tgcaaagttg ttcaaagaga acatacggca
     7021 tatatgtgcc atacatcata tacatgtgga tttcccacat cctgcgacag gtttcgacag
     7081 cgtaatcaaa gttggcaaga agttctatat tggtagcagt atccccattg acagctttta
     7141 tcttcccgta cctgccaagc atttcattaa caaacccgtc ccaaagccca tcgtagttct
     7201 caagaaaagg acggattgcg tgcctgaatt tcgcctccct ttctttcatt tcatcttcgg
     7261 aatctacaag gaggatggta aggtatcctc caccattctt aaagcgccaa tcccaaccct
     7321 tgcttcttgg aagataaagg gtctcggcgc catattgcat tccatgacgg cagaaattca
     7381 cccaaaacca tccgaacatc ggtgtccatg gcggaattga atgggtgccg tcaagaaacc
     7441 atgcaggaga ctgttcaaga tcatccttct cgtcaaattc taatcctggt acacaatcat
     7501 agatcttcat ggctattacc tctctgggga gaatgtattt tcacgaagct cataaaaaaa
     7561 atgggcacca gagtttaaac gtgcttcttc actgcagcat aaacccgagt gcctgggccc
     7621 tcaaagccat ttagaatatt ctaagtatca tgggctctga gcttgcggta acctcgtaag
     7681 acttcagtgt cggtgtgcca tcaaagtact cggtaaacat cgacaaaatc ctctggtaat
     7741 ctccgctttt ctcatatgcc tctaagtctc ccttcgtatc ccatgctgtc agagaaaggc
     7801 cctcatttac tgactccatg ctttcgaaaa gatgaacaaa cctaatcccc ttgtgtccct
     7861 tatgtgctgg tatcacatgg tttacatagg tatttctcag gttgtccatc ttccccggtt
     7921 tgactttaaa aaaagtcatt cgtacataca taatacacct ccttttcaaa gattaatgca
     7981 tagcccttcc accatcgaca agaataatct cgccagtgat aaaatcagca tcatcagaag
     8041 ccaggaacag cgcagcgcca acgatatcct ctgctacacc caatctacgg agaggtgttt
     8101 ttgaggtgtc atattttttt acatctgcaa ttgtcctgct cgcttctgta tcagtaaagc
     8161 ccggtgctac tgcattgact gtgatattat ctggcccaag ctctgctgcc agagcgcggg
     8221 tcaatccaac taccccgcct tttgaagcga cgtaatgcac aaaaccattc gaccctgtga
     8281 aaaacacttc tgaagtgaga ttgacaatct ttcctttgcc ctgtgccttc atgtgaggat
     8341 ataccgcctg ggtgcacagc caggggcctt ttacgttaat ggccatcatc atatcccact
     8401 cttcctgaga gatcttgttg aagggcctac gctccacccc aaaatagtac gcagcattat
     8461 ttaccaatat atcaatcttt ccgaattttt taacagtttc atcagccatt ctattgacgt
     8521 cttcaagctt tgtgacatca gcctgaaacc acatgcattc ggcacctttt gctgttacct
     8581 gctctgctac ttttttcaag ccttccatat cttttcgtgt caccaggcaa agctttgctc
     8641 cttctgcagc aaacctaact gcaaatgccg ctccaagccc tttacctgcc ccagtgatga
     8701 tagccacttt tccatctaat ctacccatcc cttatctcct tacacattat gatctcatca
     8761 gggcggggcc gtttcactcc ggttcccacc ccgatggcct acattttctg cttagagctt
     8821 gtatacaagc cctttgaaac catcaaccct taccatatcg ccatctttca gcaactgcgt
     8881 tcctgtgata cagtttgcaa caaccgggat atcccactct cttcccacaa tcggtccatg
     8941 ggagaggcta ccgccaccat tacagacaag acccttcagc atgggaaata cgggtgtata
     9001 cgcggattgt gatgacggac aaaccatgat atcgccaggt ttcagcttct taagatctgt
     9061 tgcggtgatt accacgcaca caggaccctc tgcaacgcct ggactgcctg ggttaccaaa
     9121 gcatgttgct cctgtagaag gatcaggcgc aacgaattct ccaatagtaa tcttcgctat
     9181 gattggatct ttcgctgccg ccataaaggc acccgcctcg tgaggctgca tttttggatc
     9241 tcttggcatg atcggtggtc tgctcaaaaa ctccttgctc cgtagccact ccatccgtct
     9301 ctccttggca ataaacccgg aatcataatt ttccggcatg gctatcatgg tttggagttc
     9361 ctcaggaatg aagaagaaga tatcatctgc ctggttgaag gtgcctactt ttgcaagacg
     9421 atcaccaatc tttaacatga caaatctgaa ggcggaaaaa caccggtgtt catacaggaa
     9481 gtcatggctt tcgctgaagg agcttgttct ctgagcgagt ctcatgaggc atttaaacca
     9541 atccatatcc ttatagccgc tggcttcagc tttctcgata atctcctttt gtgcctcgtc
     9601 tctttccctt atcagttttt cacggatcgc gtcgagagga aatggcttaa tctccctgtc
     9661 gatcatgagg tactgcttaa catggatgat ggcattctcc ggtgcctccc accatgtggg
     9721 ttctacaaac tccatcattc tgatcatacg ccatccgtac tcaaggccgt gcataaagtt
     9781 attaaggtct tcatctaccc atttttttcc ctgcggtgtt ttgttcagtt cctcaataac
     9841 tttgactgac gggttgttgg ctatgatgtt atcgatccca tactctattg ctttgttgcg
     9901 gagttcccac atggccttgt cctgccggaa gagcatattg tcatagccgc ggatcaattt
     9961 atgccatagg gttgatgtat cctttatccc cacatgatct ttgcatatgt cttcaaaata
    10021 ccagtaggcc ccatagaggg catacatgaa ccagaagtgg tcttcccaca tcctcttatg
    10081 ggtttcaatg gcatagttga actgtttgta gaggtcgtaa tatgttgcgt tttcataatc
    10141 aaaatctttc agggtgctat acctggtgag catatcattt ttgcttcttt cccagtgctt
    10201 gtcataattc ggctcaaaat cacccttgat aaactcatag aattttttac gcctgaattc
    10261 tacctcctcc ttattcggaa caggggtagc ggtacataca gccatgccgt ctatttccct
    10321 ccagtccgat gacctggttt ccggaatcca ggcttcatta cagccatata tgtgaccatg
    10381 gagcaggtta aagatccatg tccattcaaa taatggtgtc catgccgggt aggagtgaga
    10441 aaaatcgaca aaccagacag ggtatttctt ataatccctc tctggccaga attcccttac
    10501 gtgtctctta aacatccact gtcccatctt ttaccccctt ctttcattta gttttctgaa
    10561 tggtccgagc tgaggctcac ccggcaataa tgcctccaca tcacctctct ttacataaat
    10621 tttgttcatt gcccaaatat gtctacggat tcgtcgtgcc cctttctccc aaggatcaag
    10681 gctatatgcc tgtgtcgagc ctgacaacga atttaccttc attgatccct gtatcctttg
    10741 ctatgggctc acacttaacc ttcaaaagaa agattgctgc ctttccatcc gtttcccctc
    10801 tgagagcatc atagttgccg gtacccttag agatgatcag atcgcttttt cgaaatgctt
    10861 cattgacagg tgttttctca ttgccgggaa caaaaacctt gttggctgtc actaccttat
    10921 tggcaatgct atcaaggcca taaaaaacag catcatccat agtggcatcc tcgaagtatg
    10981 ccggatcctt aaccactaaa gtaacccata ggttcattgc ccttaactgg gaaatcaaca
    11041 gggaatcaaa cccaatctct cctgcattgt ctgttacata caggacgttt ttcgagttta
    11101 ccaccgtctc ataaacatta cccatgagaa caggaggcga gcccttcccc tccatcatat
    11161 ccactgcttc aggaaaggtg aaggcccctg atggagctcc cataggcgct acattgcctg
    11221 ttgccgcaag atagaaagcc ctttcaagca ttgctttcgg tgtttttgca ctttttataa
    11281 attcttcgcc tttggggagt agagatttta cgtactcgtt cgtcttctgt ttaagcttat
    11341 cccagaaggg tgagcgaggc gtgacatact cataaatgag gtcgaccgcg tcattgcaaa
    11401 tggcaccgag atttgttgag ctatccatat tgtgggcaag cagctttgta atgtttttaa
    11461 aaagctgggg aatgtccttg tttctgttct gagaaacagc cctgccgtat acccattcaa
    11521 gcagacaaag accacatttt atgtcaggct tcacaattcc ccctttctca aggggagcca
    11581 tacacgcatc atatttttcg cgagttaggc tccccttttt aaaatcaaca aactatcagc
    11641 ttatgaatct ctaccagcca cgctcttccc agggtttgta tttcaaacca aggtcctgag
    11701 caatgacctt atagcaggca tagctgctac cacgacctat gccatgagca gagtgcgctg
    11761 ctgaagagca gagatagtaa ttcttgatcg gcatgcgata tccggaaatc tctgggaatg
    11821 gtctcagcct tccaagtctc tcatagttag catcgagcgc accccaacca ccctgtggca
    11881 tacaggggtt tctgttgaca acatcatcag gtgttgcaat ccacgctgaa ataaagttat
    11941 ccatgttgac attcggtgca tatttgcccc attcctcaac cattctaaac tctatatctt
    12001 tcttaatttt cagccattgt ttctcagaaa actgccgcca tggacaggta aattcctcaa
    12061 cgagtgcggc aaaccttcca gccggcgccc tggtaggatc ccattgaaca tcaggcgcaa
    12121 caaggaggta catctggtta gcaataccca gatcccatct ctctttctgg tacctaccgc
    12181 tcaggaaata ttcaggatct gctttcccaa aatacagcct gggaaccatt cccagttcag
    12241 ggttctgcgg gtatttcggg gcatccatca aagctatatt cccccatagg agctgcgtcc
    12301 tctcatatgc tgatgtgtta taaagatcat ccggtttatc tttcagtttc tggcccttgg
    12361 cccagagttc tgcaggccag ttatccttcc ccgatttatc catggtaagt tcaatagaca
    12421 gatcactgac aacgatgtca gccagaatct tatctccatt ggttagctca ataccatagg
    12481 ccttcttgtt gtccacgagg accttattta cttcatttaa cacgaaaaac tctccaccgt
    12541 agcgttcaaa ggcccttaga agtgcatggg taatgctgtg ggtaccgccc tggacgattg
    12601 cggcaggctc catagagagg atgaggccca gggcatgaat atgccagtaa ggacctggcc
    12661 tatcctgcgg gaaaagaccg ttcgatgtct ggatggccct catgtagaag gtctgcatct
    12721 ccgggctctt gaacattgtt gttgcaatct cacccacagt tcctaacgct attttaggat
    12781 ccagcgggta tttcgggtgg ttgaagagtg cttccagggg gtcaggcccg tctccccact
    12841 ctccaggccc tgagtaacga tacttcccaa aggcctcctt ccatgccccc ttgtaactct
    12901 caattatctg cgctactgtg tctccatcat ctttattgat ttcaccaaca gacttaatgg
    12961 tgtagtcagc agcctcctgg gaaaaggagg atttaccagt aaaagggtct atcactggaa
    13021 acatagtttt accaaatata taactaccgt ccggatatat ccaagcctca ttgttttctg
    13081 gaaacacata aaccaaaccg tgatctctca attcgaaatc cgtatacgcc gggtttgtgt
    13141 aaaaccttgt aaaatgggca cacacattct ggatgtatcc cggtactggc aattcttcac
    13201 cgcatgctcc accgcccatt tcatgccatc tttcgaacat agctgtctta agcccagccc
    13261 tggcaagata acatgcaata attgtcgcat ggtgtccgcc tccaactatc actgcatcat
    13321 attttctgtc tggcatattt atttccctcc ttgttctctg tcttcatttc aattcatcat
    13381 cttgatggta ttggtggagg cccttttgga ggcgtccccg ccgctggaga agctgctttt
    13441 ggtggggcag caggcccggt acttgctgcc tgtggagccg ctgtatgcga tgtcggcacg
    13501 tcatatctgg gtcctttcat aaagagaaac ttaagaccag tcatcatcaa tccagggttg
    13561 gtgattatct tctttacaag cgatcgcaaa tcaggcggat caagaaatgc cctgacagtc
    13621 caatgcaccg gatgctgtat ttccatttcc attagcattc ttttcggttc ctttgatcca
    13681 ttcggcgctt cttccagggt tgcaggaaca agactcgtga taacaacctt tccgatcctt
    13741 cctgtaagca atgttcttcc caaaccagtt gatctaacaa acatggtatc ctccttaaaa
    13801 aagtcctagg tctttggctt cttttacacc ccgtggctct ggccaccatt ttttaatgcc
    13861 aagatcctcg gctatcttct cggcggcaca gaatccagga ccatacacaa tcatgccacc
    13921 tgaatgtgtg gacgatccgc ccacgtatag ttttttgatc ggagtgtcat gctggcaaca
    13981 gaattcattg ggtctcatga agcccatctg taagggaaga taggcaccct gtttgaagca
    14041 tccttgcttc atatcaggaa acttattctc cgtatcaagt ggtgtaccga tgtaatccca
    14101 gacaacagta tctttattca tgtttggagc gtatttctcc aggatttttt tacaccgctc
    14161 tgcatagggc ctcctcatcc tgttccatgc cagcgcaccg ccatctttca ggttgtatgg
    14221 agcacattcc atggttagga gacctatatg tttcacaaag ctgtctttat gacgatgcac
    14281 gcggatcggg tcgtggatgg aagggaaaca gcagttgaaa cctccatcat ggagttcacc
    14341 tctcttcgat gcatccagat gattaataag atcctgctca ctttcatatc cgatcagata
    14401 aataagggag ttctcgagtt cggggtgttt gtcgaagatt ttaaaatgtg gtttctctgt
    14461 caatgcgaga tgaaccgtga aaaaactcaa atctgagtat gtccactgat cgagccgtgt
    14521 gatcatatca ggaggcaggt attgcttccc tacatagtca tagaaggtct ggtgcgggtt
    14581 cagggcacta cagacaaaat gctttgcctt cagtatggtg ccgtcttcaa gttcaacccc
    14641 tttggcctcg ttgtcttcta tgattatccg tttgatcacg caaccactga taaccctgcc
    14701 tccttcttca gagatgtatt taccgaaaag gtgggctata tggtgggaac ccatgtggca
    14761 aagcctgaaa tgccaggcgc ggtttatcat gaggggtaac aggtacccca gaccttcaag
    14821 gtcataatcg agtccccaca tggtggcaca atagagcatg agtgcccgca ccttgtcgtt
    14881 ctcgaaccag gattctacaa tctgctttgg gctgtaacct gtcaactcat catcccactt
    14941 tgtgactgga tgggtttcga gttttgccac ctgttcaagg cttggcatag ggttcacgta
    15001 tgttgccggg gcaaggaaaa ggtccatagc ttcgccagag atcttggcaa agttaagaaa
    15061 cgattctgca tctttttccg aaaactgccg tattgattgt gctgtttttt caggatcttt
    15121 gtaaagggcc aggtagctgc catcctgaaa aggcatgacc atctgcaaat cagggtatat
    15181 ccactgcagg tcataccgtg tctcaaactc aaaatcgcgc agggggggtg catattcaac
    15241 cagcatatga tagatagcat gactatctac cagcataccg ggaagaagca ggttctccgt
    15301 agccaaaccc ccgccaatct cgtatcttct ttcaagaatg gcaatcttta atcctgcttt
    15361 tgtcagataa cccgccgtgg taagcccgtt ggggcccgca cctataatga tgccgtcata
    15421 ctccaattcc ctcatattca gcctccttac tgttgtaatc actcattctt acaagaatca
    15481 tcatttaacc gaataacctt ctaccttgat atcattgtat atacttctgt aatattgaca
    15541 gagcaaggca aattaattta gtattttcta agaaaaatta ttcacttgca gtgcacggtt
    15601 aggagatatg aatcttaatc agctacgagg tttttatacg gttctgaaaa cggggagttt
    15661 ttcgcgggca gccgaagaac tttgtgttac agaaccagct gtctttatcc aggtacgatc
    15721 tttagagcgt catcttggtt ttacccttct ggacaaggtc ggcaacgaac tacaccccac
    15781 agcagcaggg agaattcttt atgagtatgg agagaagatt ttcacacttg tggacgaagc
    15841 agtcaatgct gttcagggat tgcaggatct caaacatggt catctgcgtg tgggcgttac
    15901 ccaggcttta gcccagtatc tcatgcctgt tctcatcccc ggcttccagg aacggtatcc
    15961 tcacatcact gtttcaatgg atggggggag ttcgcgcgga ttggtggagg gaatcctcca
    16021 gcagcgctat gaactggccg tagttgcacg ggtaccctat ccggataaaa taacttctat
    16081 cccttataca cgggataata tcctgcttat catctctcca catcatgcgc tggcaaaaaa
    16141 ggagcaggta tcacttgctg agcttgccaa agaaccggtt atcctcaccg gtgccaagtc
    16201 cgctgtcaaa ttctccgtct ggaagggatt cgagaagcgg ggcctgcacc ctgccgccat
    16261 tattgaggcg gggaatattg aattcatcaa gcaattagtc gaaaaaggca agggatatgc
    16321 ttttcttgcc agcgtctgcg tactggaaga gatcagaaag ggggtccttg caaccgttcc
    16381 tatagaagat gagtcgttca ctgtagacat tgatgtcatt catctcaggg gcaggacctt
    16441 atcacctgca gcctccacct tccttgcctt catgcaggag cacaggaaat ctgatagctt
    16501 acgcatactt acggatgaga taacaaaaaa gactacaaca agctaaatac cttggaatag
    16561 ctgtaaaaac ttctccacca ttgcatctgc aaactccggg tcatcaatag tattgttcaa
    16621 tgcaatgatc tccactttat ctccctttat ggaatttgca aagattctga caaaagccct
    16681 gtctgtttgc gggtcaaaca gtgggccacc ggctttgctc aaagatgaga atccaaggag
    16741 aggaatgaaa acaaataccc tgctctttga tttgttaagc ttttctgcaa acacccgggc
    16801 aaccaactct acttccttcc gggaggttct tgcctgcacc cgaaatctgt caggaataaa
    16861 aagttttctc tgcgccaacc ttttttgttt ccataaagga tcggcatcct ttctgtcata
    16921 ggggccgcaa ctgagcatgt caaacccaca tggcgtgaga attacgggaa aaccagaagc
    16981 gagttctttg tccagacgat ccagtcctga cgccctgttt ccaccgagta ttgcctctga
    17041 aagccctgct ggtacaacgt caatgactcc ccttaaatat cctttctcca ccattctttc
    17101 catgattcgc tcaccagggc ctgttgctga gaatggaact gcctgatatc ctttttcttc
    17161 aagaagctca cgaacacggg cagtacatgt ctcggagaat ttgaattcgc taattgcaat
    17221 ggctggaggc cccttgccaa gcttcttttt gcttgccttg agtgtccccg ctaccattcc
    17281 ggcaaatctg tccaggatat cccttacaaa aacattgagc gtatttattt ccacgcaaga
    17341 atgaaaaacc gaaatgtcag tatcagcaaa aaatttggct gcgtatgctg gaatggctgc
    17401 agaacttgtg aggataagct tcgggatgcc aaagggaaat tctttcatta tatgagacac
    17461 tatggtaacg ttgcttgtcc ccccaatacc ggcaagacca tcaattttac cttcattata
    17521 gagctttctc gcaatgctca gaccggccag aataacataa tccatagtga gatacgtatt
    17581 ggaggtagtg gaaggcactt caaaatcaat tgcaagttca gcagtggcat ggcggcagga
    17641 aaaatcagcc actccttccc tgcacgttcc tgtggatata tcaagggtga tcacatcgca
    17701 cccctgctcc tgaatgaaac ctttcaggag cattacttct ttctcccttg tatcaaaact
    17761 ggttaagagt aaaaccgtcg gcatagtgga cccctatgat atctcactac tcaatccttt
    17821 ttccatccct attaagaata ccacaccttc caagatactg gcaacgcaac tactgtatat
    17881 ttcttgttga ttctgtttta atttccggtt acatctgaaa agatggaaag gttcatttta
    17941 ctccgtaatg ccgccctggc acctcttctt attccgagtt cattgcggtt tctcaatttt
    18001 gtcggaagaa caatcttttc ccttttttgt tcctgatgga agatacattt tgatcagcca
    18061 tgcgaaggcg agcagtatct taat
//
LOCUS       DAOOMC010000055        17685 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdus sp. isolate AMR_MDS_2685
            NODE_3322_length_17685_cov_6.351276, whole genome shotgun sequence.
ACCESSION   DAOOMC010000055 DAOOMC010000000
VERSION     DAOOMC010000055.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864760
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdus sp. (wastewater metagenome)
  ORGANISM  Syntrophorhabdus sp.
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae; Syntrophorhabdus.
REFERENCE   1  (bases 1 to 17685)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17685)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,729
            Genes (coding)                    :: 2,708
            CDSs (with protein)               :: 2,708
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 7 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17685
                     /organism="Syntrophorhabdus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3322_length_17685_cov_6.351276"
                     /isolate="AMR_MDS_2685"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2699756"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..263)
                     /locus_tag="PK125_07090"
     CDS             complement(<1..263)
                     /locus_tag="PK125_07090"
                     /inference="COORDINATES: protein motif:HMM:NF014564.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HPB37909.1"
                     /translation="MEDHEIFKDITILSLEQATVLPYLTYRLALEGARVIRMEHPVYG
                     DPNRRVGENRLNEDRMYTYFLAINCQKEAITLDLGTPEGLEIF"
     gene            724..1701
                     /gene="trpS"
                     /locus_tag="PK125_07095"
     CDS             724..1701
                     /gene="trpS"
                     /locus_tag="PK125_07095"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HPB37910.1"
                     /translation="MKRIFSGIQPTGEIHLGNYVGAIRNWVELTKDYDCIFCVVDYHA
                     ITVEYPTDEFKKRVIETALILIACGLTPDKCKVFVQSNVREHTELTWIFNCVTPIGEL
                     ERMTQFKDKSKQHRANINMGLMDYPVLQAADILIYKAGFVPVGEDQVQHVELCREVAR
                     KFNSRFGELFPEPKALLSIAPKILGVDGAAKMSKTLGNYIGLLEDEKSMWDKLRTAVT
                     DVNRVRRTDKGNPELCNIFTIHKAFSENQTLLNVDRGCRTADIGCIDCKKLLFTNLKS
                     ELGPIRERAFSLMKDTDYVIDVVRKGASDCSAIAQSTMTEVRSALGLFT"
     gene            1754..2557
                     /gene="murI"
                     /locus_tag="PK125_07100"
     CDS             1754..2557
                     /gene="murI"
                     /locus_tag="PK125_07100"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HPB37911.1"
                     /translation="MFDSGIGGLTVLKEVQSLLPTEHILYLGDTARVPYGSKSPQTVT
                     KYALESALFLLTKGIKVLVIACNTSAALSLGILKKKLPIPVLGVIDPGAKEAVSHTKN
                     KKVGVIGTKATIRSMAYERAIKRLDSGIEVVSRSCPLFVPIVEEGLEHDEVARIMAER
                     YLAEFSKSGIDTLVMGCTHYPVLEDIIHDVMGHGVAIVHTGKETAKAVKETLESDGIL
                     NTSGRGGCEFFVTDSPDLFKEVGGRFLEEPLKKVTFVKNLDYKDFLLST"
     gene            2554..4518
                     /locus_tag="PK125_07105"
     CDS             2554..4518
                     /locus_tag="PK125_07105"
                     /inference="COORDINATES: protein motif:HMM:NF036997.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB37912.1"
                     /translation="MITHVALPIPVRKTFSYLVPNTWVPFVKPFLRVKVPFKNREAVG
                     FVVGIEEGCDKGLKEIQEVIDNFPPLDKKALQLAEWASRYYIASQGLVLGYALPPGLP
                     IEQYLKIKVLAEIEPSGLDNLPLRKAYRLFGRDVVYKHYSNGLLRYYDTFTNKDFIPC
                     SHEDVSDRGGKTLFLGNTNDRLQYYIIKIAQCLEEGKNVLMFVSNHHTIGQFCFDRLT
                     EAFPGKVIRYGVSVTSKKRMETYFRARNEGGQIILGSRSCLFLSVLRNGLIIVERPEE
                     DDYRNEAGFRFNAVNLAIKRAEIENVPIILGSASPPLDVYKRAVDGEINVIEKVYTIK
                     KQPAEIIIEKGISTLGALPDELKSLLLQAVERNEKVAIYTPRKDYSSRIRCLDCKGLF
                     LCQVCEGSLSYQKQRDALSCASCGKKFPYEECCRQCGSRLIQFSNVGVEYLEQTLGEA
                     FPGVTIISAIGESLYENKEAFTKLSSREPVFVIGTQALAKPYGVKVDKLVMIEWEELQ
                     RIGGYRAMEKMFHILSSLVDVLEPDEIYAVMVRKKRVDLKGFFEIKTFYSGELEKRKN
                     AQFPPFVRIFLLELEKENEVVGLRLVEKIKAAASEQGIIQHITGPLVQKRKKYRWRMI
                     IRGNEEQLHDFLATVSIFPGVRVEADPVNI"
     gene            complement(4684..5124)
                     /locus_tag="PK125_07110"
     CDS             complement(4684..5124)
                     /locus_tag="PK125_07110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB37913.1"
                     /translation="MKKALLIVLAALISAFVPTVFAQAKSEAKQADRSVTASHEKTQV
                     FEKKPDKPVEKTAPKAKTIKGEFVSMDAAARTMVVRDAKGEMTFDVTGIKRLVEFKGG
                     DKIMVAYTEKEGKMIAQFVLKKAVKKDASKKEEIKTVPAPEKKQ"
     gene            complement(5286..6776)
                     /locus_tag="PK125_07115"
     CDS             complement(5286..6776)
                     /locus_tag="PK125_07115"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HPB37914.1"
                     /translation="MRHDKGSVLVVEDDRYQREIIKTILAKEGFYVETTDTGKKAIDS
                     LKSGTFDVILTDLRLPDIDGTEVLKEAKSFNRPNHVIIITAYGSIPSAIEATKLGAFY
                     YLEKPFEKDQLLLVINNAVNQVKLLNDNIMLKDQLAGRFHLDNIIGVHGRMEELFKVV
                     GKVAPTNSTVLIYGESGTGKELFAKSIHYNSPRKNKLFFAINCAAIPETLLESELFGY
                     EKGAFTGALARHTGLFEQANGSTLFLDEIGDLTLGTQAKLLRVIQERELRRIGGKENI
                     KLDVRIITATNKNLEQEMAAGKFRQDLYYRLNVIAFTIPPLRERLTDVPILAEHFLKR
                     LNASHEDKKHISQDVLNALLVYTWPGNVRQLESVIERAYVMCDGETINLEHIPEEVKQ
                     SSLAGPLLRELQTNFDSLIDAKLTGAEYRLYLYLSRLNPFDENFQQRMEPKSIIERLG
                     INRDTFFVGIAKLKELGLYDFKAKASQAKTISPNKSQKRNLQEIPD"
     gene            complement(6766..8229)
                     /locus_tag="PK125_07120"
     CDS             complement(6766..8229)
                     /locus_tag="PK125_07120"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPB37915.1"
                     /translation="MARSETVISVKKVFRSFPLKTQLLLILLFLLLISISSLTIIYSR
                     SEEMLLDKVSDNIEDITKAIQISVEELTYRGDSGARLKGYVDMLNKKGIKEISIMSDT
                     AEVIASSNPKKIGTREKLGEKKNKKKDLMITARLGEENKKESQRLYNIIMPVQIKGQN
                     IGYILISMVLDDYKALSKKNHLKRVLSTLFVFSIGIIFSLLIAEKYTEPIKRIADASK
                     KIAAGQLVKIKGSSRKDEIGVLIRSYNDMVEKLAERMELEEKLKKSEQLSIIGQLSSG
                     IAHEIRNPLNFLSLSIGHIKESILAEKIHDKDGLSKLLDDLIKEIHKVNDLIHNFLFL
                     GKPITLRKDWVMPGTMIDEVLYIVKDKIRKGIDVKVQCDNGLPMYCDSEYIRLCIMNL
                     LMNAVQAIEGKGEITIGCGRENGFSLIFVSDTGAGISHEDKAKVFEPYFSTKKFGIGL
                     GLAISKRFVEEHGGAIVIESEVGKGTTIKLRIPDNEA"
     gene            complement(8199..9563)
                     /gene="gltX"
                     /locus_tag="PK125_07125"
     CDS             complement(8199..9563)
                     /gene="gltX"
                     /locus_tag="PK125_07125"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR00464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HPB37916.1"
                     /translation="MVKVRFAPSPTGNLHVGNLRTAVLNYLYARNQGGTFILRIEDTD
                     MERSEARYETSIMDDLKWLSINWDDGPYRQSERTDLYRDFANTLLEKGLAYKCFCPKE
                     KLENARHASLDKGEPPRYDGRCRALSPEELKSFEEEGKPYVVRFRAPRKAIFFRDGIR
                     GQIEFPPDHVDDFILLKQELTPSYNFAAAIDDMTMGITHVIRGGDHISNTPKQIMLFE
                     AFRKKPPKYAHHSLLVGNDKKPLSKRHGATRITEFRDMGILNVALLNYMGILGRGVMK
                     EVMNEDELARTFTLKSLSGSDSVFDMEKLFWFNKEHIRRMPVERLLEVTGLDQTYSEK
                     VFLLRENARTIVEIRELLSIFEGTEIADSGLSYLASSSCFQAVVAVVRNALNDSRDIT
                     LEDILEKVVHTVDAPRKELFLVLRILITGRTDGPPLKDIFHFIPSVHILERIRWLDQR
                     LSSQ"
     gene            complement(9630..10112)
                     /gene="ispF"
                     /locus_tag="PK125_07130"
     CDS             complement(9630..10112)
                     /gene="ispF"
                     /locus_tag="PK125_07130"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HPB37917.1"
                     /translation="MRVGVGYDAHRLVEGRKLFLGGIEIPFHKGLLGHSDGDVLIHAI
                     CDAILGAISEGDIGTHFPDTDSSTKGIESVKILRHVIGLASKKGFTVVNVDAVVAAQE
                     PKIAPVRDAMRKSMAAILGTGEDRISIKGKTTEDLGFVGRGEGIEVYAVVLLEKSGEK
                     "
     gene            complement(10109..10807)
                     /gene="ispD"
                     /locus_tag="PK125_07135"
     CDS             complement(10109..10807)
                     /gene="ispD"
                     /locus_tag="PK125_07135"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: protein motif:HMM:NF013308.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HPB37918.1"
                     /translation="MRALAIILAAGVGKRMGAATNKQLLFLDNKPIVVHTLQVFEDCK
                     AIDGVYLVVNQKDLPVVQEEILETYRFNKVMKLVIGGRLRQDSVKNGLEAIEHPCDIV
                     VIHDGARPFITPSFIEKGIFLMEMFDAIIPAIPVKDTIKVVSKEGFVIKTLERDSLWH
                     VQTPQTFKFDLISKAYKDGISKKLCGYDDAAFLEFTGKKVKVIEGSSYNIKITTPEDL
                     VTARGILAQLKGDS"
     gene            complement(10808..11854)
                     /locus_tag="PK125_07140"
     CDS             complement(10808..11854)
                     /locus_tag="PK125_07140"
                     /inference="COORDINATES: protein motif:HMM:NF014047.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAM domain-containing protein"
                     /protein_id="HPB37919.1"
                     /translation="MDIIVQIILVIVTTTTSYFILKEIAGGTLWGAIGAICGLALGYV
                     IIKLESKLKDISLKTIVCSLTGVMISLLVAHLFISQLLLTLVKDIPITLPIYILFYFI
                     MGYLGFKIGKEKSKTLDLSKVPLLDKMESAEDVKILDTSTIIDGRIADICDTGFLEGT
                     FIIPQFVLYEIQHIADHQDPVKRTRGRRGLDVLHRLQKQMFVKVKIVDYDFPKLQDVD
                     SKLVALAKKLTGKLVTNDFNLNKVAELQGVEVLNINQLATSLKPAILPGEQMNIKILK
                     EGKEYGQGIGYLDDGTMVVVDEARKLLGRQVDVVVTSVLQTTSGRMIFGKLKELADRE
                     YRYPEEIKVEEQVR"
     gene            complement(11860..12354)
                     /locus_tag="PK125_07145"
     CDS             complement(11860..12354)
                     /locus_tag="PK125_07145"
                     /inference="COORDINATES: protein motif:HMM:NF014602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HPB37920.1"
                     /translation="MFEVGEVAVYPGHGVGKIESIEEKEFSGTKQSFYIIRIMDTDMT
                     IMIPVDGAKNAGLRGVIDSRDVTKVLNILKEKNSIHDNAPWNRRYKEYMERIKSGSIF
                     EVAMVLKELYSLRVWKELSFGEKKMFEIARNLIKKELSISLDKEEEEIEQEIERIFLK
                     NYKE"
     gene            12645..14036
                     /gene="gltX"
                     /locus_tag="PK125_07150"
     CDS             12645..14036
                     /gene="gltX"
                     /locus_tag="PK125_07150"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR00464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HPB37921.1"
                     /translation="MIKTRFAPSPTGNLHIGGARTALFNYLYAKHTGGVFVLRIEDTD
                     IERSKEEYTKDILEGLKWLGVYWDEGPHFQKERMDIYRQHAHRLLNEGKAYKCYCSTE
                     TLEEKRKAALREGRKPNYDRTCRDLTEDRKNQPFVIRFKSPVNGEVSFEDRIRGRITF
                     KCEELDDLVILRTDNTPTYNFTVVVDDALMGITSIIRGDDHINNTPRQILIYEALNYE
                     TPEFAHVPLIHGKDKSRLSKRHGATSLLEYRNDGFLPEALMNYLARLGWAHGDQEVFS
                     REDLIEKFDLGKVGKSPSIFDMDKLLWLNGHYLKTMPEGDIAARLEPFIENRGIKVTD
                     KNKLIPVVINLRDRAKTLKEMAEMAAFFFTDDFPYDENAKNKFLTVSTKPILERFLNE
                     FTSVSSFDENEQRKLFETMAQDGGKKLVEVIQPVRVALCGRTVSPGIFEVVSILGRDS
                     VVKRIKKAIASIA"
     gene            14033..17368
                     /locus_tag="PK125_07155"
     CDS             14033..17368
                     /locus_tag="PK125_07155"
                     /inference="COORDINATES: protein motif:HMM:NF014516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HPB37922.1"
                     /translation="MRLHRLELFGFKSFYNRTVFQFDEGITSIVGPNGCGKSNVVDAM
                     VWALGERGTKTLRVKDMGDVIFHGSNGKKPVNIAEVTIDLTDDGKDISVKRRVYRDGT
                     SEFFLNGEAVRLKDVQDFFLGTGIGVNSYAIVEQGRIEYIIQMKPQERRIVVEETSGI
                     TRFEEKKRDAIARLTEVSSNLERVEDIYTEVKASFEKAEVEWNRWKIYKELAHKQNEI
                     DKWILLDGFMKVKKRMGKIMERHEEIDREFAQKEAEKIKLREELEAKEDEFSLVDTTS
                     RKLEVDIKGKEKDMENRLLEIEYLKGELVRLQEEQNGLAKAREDLEKTSAIYQKDIES
                     LTASRTDSETLLLDIENEERGLQESLETQKKKVADHEAKIEAERGDLFVAMSKLTETK
                     NRILEIERQAQERKKREAKRAEELGRLNRRLAQLELSRSTLQDRMDISKAERDRVAGE
                     EVAAFAEREEITRLIDTERRAIDLLKSEKLAKEEFLKQMASIKGVGGKPIPDTKRLID
                     LVRAEETREKALERFFFKELEYHVLRGSDASAIADLVQKYEGNFIFFPKKGIFRQSSD
                     EVELDVKWIRDINEGLKRIEDGEEGIFINDDVLIDSRGLVLREKEEKKVDLKQFRERK
                     KAEKALKEIEENIAKKLEAMKEHQDALGKQGAIWKKWKSQKDDIDKALHSLEREFLLI
                     DAENKLVKERLAELNTEIDLFEEAPQDSIESLLEEQKVREGDRQKREGNMAALRQELD
                     EVKAEYENIRAHKQEAAISIERHRNALKTLDEDIRRKLVLIKTAQEDRERITAKIEKA
                     ARSCEEVTARAETLEEDYAHIKETCEKSVQRYEGLKVSAGNLHMEKQILQERVNTIIK
                     EMERVRNRKEGGEKELAVLSEKRNTIAERLATVYGIDTPENVPVPHGEDFEGEREKIV
                     AEIAELGDVNFRAEMEYTELKDKTAFLEQQKEDLRNAMDSLKKTIAKIDSLSKDIFIE
                     TFDVVNNAYKKFVQLLFKGGHGYLAVSQDGSGIEMYVQPPGKKVLRMEQLSGGEKALV
                     SLGFLLALMDTKPSPFSLMDEIDAPLDDANIIGLMEIIKDISKKTQIIFITHNRITME
                     CSNTIYGVTMEDEGVSKVVSVRL"
BASE COUNT         4962 a         3841 c         4079 g         4803 t
ORIGIN      
        1 ttgaatattt ctaaaccttc gggagtacca aggtccaggg ttatggcttc cttctgacaa
       61 ttgatggcaa gaaaatacgt gtacattctg tcttcgttaa gcctgttttc gccaaccctc
      121 cggtttggat caccgtaaac cgggtgctcc atacggatga cccttgcacc ttcaagggca
      181 agcctgtacg tcagataagg caagactgtt gcctgttcaa gagacaaaat tgtaatatcc
      241 ttgaagattt cgtgatcttc cataataacc ccccacttgg atattgtata caatttacaa
      301 catcaactat agtattatga caataaattg tcaagatttt tgtatacgaa attaagacag
      361 gtaatgggcc aggggcaatg acgacaggac atgggaaaat agatttcagg gtaaaactga
      421 cagcatgtat gatgctcttc acccatgaat gctgtgccac gaaacgtgaa catcaagact
      481 aaaatccata tcgatatcat ggattccggt tgcacttttg ataccattag ctttaaccct
      541 gaaactcgtt gattttaaga gccttttagt gtagattaac cttcagcata ccctagtgat
      601 gaacaattct ttaaatcaca ggatgtcagg gggttaaaag ttccgggcat tattctccga
      661 acaccgaact ccaaacatta gagccagggt cgctaaattg aaacgaattt tcacggagga
      721 aaaatgaaac gaattttcag tggcattcaa cccactggcg agatacacct tggaaactat
      781 gtcggagcta tccgtaactg ggttgagctt actaaagatt atgactgtat tttttgtgta
      841 gttgactatc atgccattac tgttgaatat ccgacagatg agttcaagaa acgggtcata
      901 gaaacagccc tcatcctcat agcctgcggg cttacccctg acaagtgcaa ggtctttgtc
      961 cagtccaatg tgagagaaca tacagagttg acgtggatat tcaactgtgt cacgcccata
     1021 ggcgagcttg aaaggatgac ccagttcaag gacaaatcga aacagcacag ggcgaatatc
     1081 aacatgggcc ttatggacta ccctgtcctg caggctgccg atatcctcat ttataaggcc
     1141 ggatttgtgc cagtcgggga ggaccaggtt cagcatgtgg agctttgtcg cgaggtagca
     1201 agaaaattca atagcagatt tggagagctg tttccggaac caaaggcgct cttatcgatt
     1261 gcgcccaaaa ttcttggcgt tgatggggcg gccaagatgt caaaaaccct tggaaattac
     1321 atcggcctcc tcgaagacga aaaatcaatg tgggataagt tgaggacagc agtcaccgat
     1381 gtcaacaggg ttcgcaggac agacaagggt aacccggaac tctgcaatat cttcaccatt
     1441 cacaaggctt tttctgagaa tcagactctc ctgaatgttg ataggggatg cagaactgct
     1501 gatattggat gcatcgactg caaaaagctt ctttttacga acctgaaaag tgagcttggc
     1561 cctatcagag agagggcatt ctccctgatg aaagatactg attacgtaat tgacgtggta
     1621 cggaaagggg catctgactg cagtgcaatc gcccaaagta cgatgacaga ggtgcgcagc
     1681 gccctcgggt tattcacttg acatttaaac tcgtctacca tactcttttc gagtgaaaaa
     1741 gctttccgtt ggcatgtttg attccggaat tggagggctc actgtcctta aagaggtgca
     1801 aagcttactt ccaacagaac acattcttta tctcggtgac acagcccgtg tgccctatgg
     1861 cagcaagtcg ccccaaaccg tcacgaaata tgcccttgag agcgctttgt ttctgcttac
     1921 caagggcata aaggtacttg tcatagcctg caatacctcg gcggccctga gcctgggtat
     1981 actgaaaaaa aaactcccta tcccagtcct tggtgttatt gatcccggcg caaaagaggc
     2041 agtttcccac acaaagaaca agaaggtcgg tgttattggg acaaaagcaa ccatacggag
     2101 tatggcatat gagagggcaa ttaaaaggct cgattcggga atcgaagttg tttcacggtc
     2161 atgccccctg tttgtgccaa ttgttgaaga aggtcttgaa catgacgagg tggcacgaat
     2221 catggcagag cgctatctcg cagagttcag caaatctggt attgatacgc tggttatggg
     2281 ctgtacccac tatcctgtct tggaagacat cattcacgat gtcatgggtc atggtgttgc
     2341 aatagttcac acgggaaagg agacagcaaa ggcagtgaaa gagactctcg aaagcgatgg
     2401 tattctgaat accagtggta ggggtggttg tgaattcttt gtcactgatt ctcctgacct
     2461 ttttaaagag gtgggaggga gattccttga ggaacctctc aaaaaggtaa cctttgttaa
     2521 gaaccttgat tataaagact ttctcctctc tacatgataa cccatgtagc attgcctata
     2581 cctgtaagaa agacattttc ctaccttgtg cccaacactt gggttccctt cgtaaaaccc
     2641 tttctccgtg taaaagttcc ctttaagaat agggaggctg tcgggttcgt tgttggtata
     2701 gaagaaggtt gtgataaggg cctgaaggaa attcaagagg ttatagataa ttttccacct
     2761 ctcgacaaaa aggctctcca actggcagaa tgggcatctc gctactacat tgcgtcacag
     2821 gggcttgtcc tgggatatgc cttacctcct gggttaccaa tagaacaata tctgaagata
     2881 aaggtattag cagaaattga acccagtggt cttgacaatc tcccgctcag aaaagcatac
     2941 agactgttcg gccgggatgt tgtctacaaa cattacagca atggcctcct tcgatattac
     3001 gataccttta cgaataagga ttttatacca tgcagtcacg aagacgtaag tgatagaggg
     3061 ggaaagacgc tttttctcgg gaataccaat gaccgcctgc agtactatat aataaaaatc
     3121 gctcagtgtt tggaagaagg taaaaatgtt cttatgtttg tatctaatca ccacacaatc
     3181 ggacagtttt gctttgacag gctcacagaa gcattcccgg gaaaggtgat tcgttatggg
     3241 gtttcagtga cttctaaaaa aagaatggaa acctacttca gggcgaggaa tgaaggaggt
     3301 caaatcattc tcgggagcag gagctgtctt ttcttatctg tccttcggaa tgggcttatt
     3361 attgtggagc gaccggagga agatgattac agaaatgaag caggtttcag gtttaacgct
     3421 gtcaatcttg ctatcaaacg agccgagatt gaaaatgtcc ctatcattct cggaagcgcc
     3481 tcgcccccgc ttgatgtcta taaacgagca gttgacgggg agataaatgt tattgaaaag
     3541 gtatatacca taaaaaaaca acctgctgag atcattattg aaaagggaat atcgacatta
     3601 ggggcattgc ctgatgaact aaaatcattg ctgttgcagg cagttgagag gaatgagaag
     3661 gtagctattt ataccccgag gaaggattac agctcccgca tcaggtgtct ggattgtaaa
     3721 ggtctttttc tttgtcaggt atgtgagggc agtcttagtt atcaaaaaca aagggatgcc
     3781 ctttcctgtg ccagttgcgg aaagaagttc ccctatgaag aatgttgccg tcagtgtggg
     3841 agcaggctca tccagttttc caatgtgggg gttgaatatc ttgagcaaac acttggtgag
     3901 gcttttcctg gtgtcacaat tatttcagca atcggcgaaa gcctttacga aaacaaggaa
     3961 gccttcacaa agctcagcag cagagaaccg gttttcgtta ttgggaccca ggcacttgcg
     4021 aaaccatatg gtgtcaaggt agacaagctt gtcatgattg aatgggaaga gttgcaaagg
     4081 ataggtggtt atcgtgccat ggagaagatg tttcatatcc tttcaagcct cgtagatgtg
     4141 cttgagcctg atgagatcta cgcagtcatg gtgagaaaaa aaagggtgga tctcaagggg
     4201 ttttttgaaa taaaaacatt ctatagcggg gagcttgaaa agagaaagaa tgcccagttc
     4261 ccgccttttg tcaggatttt tttgctggaa cttgagaagg agaatgaggt tgttgggcta
     4321 cggcttgttg aaaagatcaa ggcagcagca agtgagcaag gtattatcca gcatattaca
     4381 ggccctctcg tacaaaaaag aaagaaatat cgctggagaa tgatcatcag gggcaatgaa
     4441 gagcaacttc atgactttct tgctaccgtc agtatttttc cgggggtaag ggtagaagct
     4501 gacccggtga atatataaaa acgtttatgg tcacttgcat attgtagatc aaaagaagac
     4561 cgcatggttt cgcctatgta gtgtatctcg ttttttacga aatacgatgc ataagatgcc
     4621 ctgtacagcc aatgggatat tggaaaaaaa gggccgaagt ccccttttgc attgattaca
     4681 gatttactgc ttcttttccg gtgcagggac tgtcttaatc tcttcctttt ttgaagcgtc
     4741 cttcttcaca gcctttttca atacaaactg tgctatcatc ttgccttctt tctcagtata
     4801 agcaaccata atcttgtcgc cacctttgaa ttctaccaat ctctttatgc ctgttacatc
     4861 aaacgtcatt tccccctttg cgtcccttac caccattgtt ctggctgccg cgtccatact
     4921 gacgaactca cccttgatgg tctttgcctt cggtgcagtc ttttcaacag gcttatctgg
     4981 ttttttctca aagacctggg tcttctcatg ggaagctgtc accgacctat cagcctgttt
     5041 tgcctcagat tttgcctgtg caaataccgt ggggacgaat gcactgatca gcgctgcgag
     5101 aacaattaat aatgcttttt tcataatcct gttatctcct gtaaagattt aatttagtat
     5161 catggaaaac tattttgtat ttgccatgcc agacgaataa aaataactta caaaatctac
     5221 atggtaaaca tagcaatagg ccccgactga tttgctgcag gacaattccg actatatttg
     5281 caagattaat cgggaatttc ctgcagattt cttttctggg atttgtttgg ggaaatggtc
     5341 ttggcctgac ttgcttttgc tttaaaatca tagaggccga gttctttcag ttttgcaata
     5401 cccacaaaga atgtgtctct attgattcca agcctttcga tgatggattt aggttccatc
     5461 ctctgttgga agttctcatc gaaaggattg agccttgata gatagagata gagtcgatac
     5521 tctgctccgg ttagctttgc atcgatgaga gaatcgaaat tggtttgtaa ttctctcaat
     5581 agtggtcctg caagggagga ttgttttact tcctcaggga tatgttccag gttaatagtc
     5641 tcgccatcgc acatcacata ggcccgctca attacagact caagctggcg gacatttccc
     5701 ggccaggtat acactaagag tgcattcaaa acatcctgtg atatatgttt tttgtcttca
     5761 tgtgaagcgt tcaagcgttt gaggaagtgt tcagccagaa tggggacgtc agttaatctc
     5821 tccctgagag ggggtatggt aaaggctatg acattcagcc ggtaataaag gtcctgacgg
     5881 aattttccgg ctgccatctc ctgctcaagg tttttgtttg tagctgtaat tatcctaaca
     5941 tcaagcttga tattttcttt gcccccaatt cttctcagtt ccctctcctg gatcacgcgg
     6001 agaagctttg cctgggtccc aagggtaaga tctccgattt catcgaggaa gagagtactc
     6061 ccgtttgcct gttcaaaaag tcctgtatgc ctcgcaaggg caccggtgaa agcccctttt
     6121 tcgtacccaa agagctcgct ttccagaagt gtttcgggta ttgccgcaca gttgatggca
     6181 aaaaaaagct tgttctttct tgggctgttg tagtgaatgc ttttggcaaa gagctctttt
     6241 cctgtaccgc tctccccata aataaggacc gttgagttag ttggggcaac ttttccaaca
     6301 accttaaaaa gctcttccat acggccatga acgcctataa tgttgtccag gtgaaacctg
     6361 ccagcaagct gatctttcag catgatgtta tcgttgagaa gctttacctg attcactgca
     6421 ttgttgataa caaggagtag ctgatctttt tcaaaaggtt tttcaagata atagaaagcc
     6481 ccgagttttg ttgcctctat cgctgacggg attgaaccat aggcagttat gatgataacg
     6541 tgattggggc gattgaatga cttggcctct ttcagtacct cagtgccatc aatgtcaggg
     6601 aggcgaaggt cagtaaggat tacatcgaat gttccgctct ttaaagaatc gatcgctttt
     6661 ttccctgtgt ctgttgtctc cacataaaag ccttctttgg cgagaatggt ctttataatc
     6721 tccctttgat acctgtcatc ctccaccacc aggacgcttc ctttgtcatg cctcattatc
     6781 cgggatcctt aattttattg ttgttccttt tccaacctca ctctctatga ctatggcacc
     6841 cccgtgctct tccacaaaac gtttgctgat tgcaagacca aggccaatcc cgaatttttt
     6901 ggtggaaaag tatggttcaa agactttggc tttgtcttca tgacttatac ctgccccagt
     6961 atcactcaca aagataaggg agaacccatt ctccctgcca caaccaatgg ttatttctcc
     7021 cttcccttct atagcctgaa cagcattcat gagaaggttc atgatacata accttatgta
     7081 ttcactgtca caatacattg gcaatccatt atcgcattgc actttcacat caatgccctt
     7141 cctgatttta tcctttacaa tataaagaac ctcatcgatc atggtgccgg gcataaccca
     7201 atcctttctc agagttatag gtttaccgag aaaaaggaag ttatgtatca ggtcattgac
     7261 cttgtggatc tcctttatga gatcatcaag cagctttgaa agaccatcct tgtcatgaat
     7321 cttttctgcg aggatgctct cttttatatg cccgatagac aatgagagga aattgagcgg
     7381 attccgtatt tcgtgggcta tacctgaaga aagctgccca atgatggaga gttgctcgga
     7441 tttcttcagc ttttcctcaa gctccattcg ctctgccagt ttttccacca tgtcattata
     7501 gcttctgata agtacgccaa tctcatcttt acggctgctc cctttaatct taacaagctg
     7561 acctgctgca attttcttgc ttgcatcagc aattctcttg atgggttctg tgtatttttc
     7621 cgcaataagg aggctgaata tgatgccaat actgaaaaca aagagggtgc tcaggactct
     7681 ttttaaatgg tttttctttg acaaggcctt gtaatcatca aggaccatgc tgatgaggat
     7741 gtacccgatg ttctgcccct taatctggac aggcattata atgttgtaga gtcgctggct
     7801 ctcctttttg ttttcctcac cgagacgggc tgtgatcatt agatcctttt ttttgttctt
     7861 tttctcccca agcttttctc tggttcctat tttctttggg ttagaactgg caataacctc
     7921 tgcggtgtca ctcatgatgg agatttcctt aatccccttt ttgttgagca tgtccacata
     7981 gccctttaat cttgcgccac tgtctccgcg atacgtgagt tcttcaacgc tgatctggat
     8041 tgcttttgta atgtcttcga tattgtcgct cactttgtcc aggagcatct cttcagatct
     8101 tgaataaatt atcgtgagag aacttatgga tataaggagg agaaaaagca ggatgagaag
     8161 gagctgtgtt ttgaggggaa agctcctaaa aacctttttt actgagatga cagtctctga
     8221 tctagccatc gtattctctc aaggatgtgg acactgggga taaagtgaaa aatatctttc
     8281 agaggagggc cgtcagtcct tcccgtaatc aaaatcctga gaactaaaaa aagctctttc
     8341 ctgggagcat ccaccgtgtg aaccactttt tccaagatat cttcaagggt aatatcacgg
     8401 ctatcgttga gagcgtttct caccaccgct acaactgcct ggaaacacga agagcttgca
     8461 agataactta aaccactgtc ggcaatttct gtgccttcaa aaatagagag gagctctctt
     8521 atttccacga tggttcgtgc attctcccgt aaaagaaata ctttctctga atatgtttga
     8581 tccaatccgg ttacttcaag gagtctctca acaggcattc tgcgtatatg ttccttgttg
     8641 aaccagaaaa gcttttccat atcgaatact gaatcagagc ccgagagaga ttttaaggta
     8701 aaagtgcgtg caagctcatc ttcattcatc acctctttca tgacacccct gccgaggatg
     8761 cccatataat ttaacagggc aacattcagg atgcccatgt cgcgaaattc agtaatccgt
     8821 gttgccccgt gtctcttgct caagggtttt ttatcgtttc ctacaagaag agagtggtgg
     8881 gcgtatttgg gaggtttttt cctgaaggcc tcgaaaagca tgatctgttt tggtgtgttc
     8941 gatatatgat cgccacccct gataacatgg gtgatcccca tggtcatatc gtcaattgcc
     9001 gctgcgaagt tatatgatgg tgtgagttcc tgttttaaaa ggatgaaatc atccacatga
     9061 tcaggtggga actcgatttg tcccctgatt ccatccctga agaatatagc ctttcttggc
     9121 gccctgaacc ttaccacata aggcttgccc tcttcttcga aagatttcag ttcttccggt
     9181 gacaaagccc tgcacctgcc atcataccgc gggggttcgc ccttgtccag ggatgcgtgc
     9241 cgtgcatttt ccagtttttc cttcgggcag aaacacttgt acgccagacc cttttcaaga
     9301 agtgtgtttg caaagtcccg ataaaggtca gttctttcag actgcctgta cggaccgtca
     9361 tcccagttga tggagagcca tttcaaatca tccataatgg aggtctcata ccgggcttcc
     9421 gaccgttcca tatcagtgtc ttcaatgcgg agaataaatg taccaccctg gtttctagca
     9481 taaagatagt tgagaaccgc tgtcctgagg ttaccaacgt ggagattgcc agtggggctt
     9541 ggggcaaatc gtactttaac cacgggcatt gcctccaggc tggtgccggg tgttaagcgt
     9601 atagcacctg gcgttcagta tcttccttat catttttccc ctgatttctc cagcaggacc
     9661 acagcataca cttctatgcc ttcccctctt cccacaaacc caagatcctc agtagtcttg
     9721 cccttgatac tgattcggtc ttcaccagta ccaagaattg ctgccatact cttgcgcatg
     9781 gcatcccgca ccggggctat tttgggttcc tgggcagcta caacagcatc aacattgacg
     9841 acagtgaagc cctttttgct ggcgagacca attacgtgcc tgaggatttt aacactctcg
     9901 atgccttttg tggaactgtc agtgtcagga aagtgggtgc caatatcgcc ttcgctgatt
     9961 gcgccaagaa tagcatcaca gatcgcgtgg ataagcacat cgccgtcgga gtggcctaaa
    10021 agacccttgt gaaaggggat ttctatcccg ccgaggaaga gcttccgacc ctcaacaagc
    10081 cgatgagcat cataacctac accgaccctc atgaatcgcc ttttaactgg gcgaggatac
    10141 ccctggctgt caccagatct tcaggcgttg tgatcttaat attgtacgaa gaaccctcga
    10201 tcaccttcac ctttttccct gtgaattcca gaaaggcagc atcgtcatac ccgcagagct
    10261 tcttcgagat tccatctttg tatgcttttg atatgagatc aaatttaaat gtttgcggtg
    10321 tctggacgtg ccacagcgag tcacgctcaa gggtctttat cacaaagcct tctttcgata
    10381 caacctttat ggtatctttt acgggaattg caggaatgat tgcatcgaac atctccatga
    10441 gaaatatccc tttttctatg aaagagggag taataaaagg acgggcgcca tcatggatca
    10501 cgactatatc acagggatgc tcgattgcct caaggccatt ctttacagag tcctgccgga
    10561 gccgtccccc gataacaagt ttcataacct tgttgaaccg gtatgtttca agtatctctt
    10621 cctgcacaac aggaaggtct ttctggttta ccacaaggta cacaccatct atagccttac
    10681 aatcctcaaa aacctgcaac gtatgaacaa caattggttt gttgtctaaa aagagaagtt
    10741 gtttattcgt ggcagcaccc attcttttgc ctactccggc tgccagtatg attgcaagag
    10801 ccctcattta cctaacctgc tcttctacct tgatctcctc agggtaacgg tactccctgt
    10861 ctgccagttc cttcagtttc ccaaaaatca tccttcctga tgttgtctgg agaacgcttg
    10921 taaccaccac atcaacctgc cttccgagga gcttgcgtgc ttcatcaaca acaaccatag
    10981 ttccatcatc aagatagcct attccctgac catattcctt accctctttg aggattttga
    11041 tgttcatctg ttctccgggc agtatggcag gcttaagcga tgttgcgagt tggttgatat
    11101 tgagtacttc aacaccctgc agctcggcaa ccttgttgag gttaaagtca ttggtcacca
    11161 acttgccggt aagtttcttt gcaagggcaa cgagcttgga gtctacatcc tggagttttg
    11221 gaaaatcgta gtccacaatt ttaactttta caaacatttg tttctgaagc ctgtggagca
    11281 catcgaggcc ccgcctgccc cttgtcctct tcacggggtc ctggtggtct gctatgtgtt
    11341 gaatctcata aagcacaaac tgggggataa tgaacgtccc ctcaagaaaa ccagtatcgc
    11401 atatgtctgc aattctcccg tcgatgatgg tgcttgtgtc gaggattttg acatcttcag
    11461 cagactccat cttgtcgagc aggggcacct tggaaagatc aagggttttg ctcttttcct
    11521 tccctatctt gaaaccgagg taacccatga taaaataaaa gagaatgtag ataggcaggg
    11581 tgataggtat atcttttacg agggtgagca ggagttgtga tatgaagaga tgggcaacca
    11641 agaggctgat catgacaccg gtcagactgc atacaatagt cttcagagag atgtccttta
    11701 gcttgctttc gagcttgata atcacataac cgagtgcaag tccacagatg gccccgatag
    11761 caccccagag ggtgccaccg gctatctctt tcaaaatgaa ataacttgtt gtggttgtta
    11821 cgattaccag aataatctga acgataatgt ccaaggagtt tattccttat aatttttcag
    11881 gaagatccgc tcaatctcct gttcaatctc ctcttcttcc ttatctaatg aaattgaaag
    11941 ctcttttttt ataaggttcc tggctatttc gaacatcttc ttctctccaa aggagagttc
    12001 tttccaaacc ctcaagctgt aaagttcttt caacaccatg gctacttcaa aaattgagcc
    12061 gcttttaatc ctttccatat actccttgta acgtctgttc cagggagcgt tatcatgaat
    12121 tgaattcttt tccttcagga tattgagaac tttagtaaca tctcgggagt ctatgacgcc
    12181 acggaggcct gcattttttg ccccatcaac aggtatcatg atggtcatat ccgtatccat
    12241 gatcctgatg atatagaagg actgcttggt cccagagaac tctttttcct ctatagactc
    12301 aatctttcca acaccgtgtc ctggatatac tgcaacttct cctacttcaa acattctccc
    12361 tccaatcatt aactgattgt tttttgtggt ttttatcgat tgttattgta ccattttgtg
    12421 gaacaaaatt caaggatgat gtatttttgc tacttcgcag catgctcata gcccgcagct
    12481 tctaccgatc agcaaaaaaa gaacgatgca gcaagaacga gtctttgatt tttcagtgct
    12541 tacagcttac agctatgagc ttaaagctgc ttctggtaaa atttcgttga agcaaggggg
    12601 attttctatt ataatcagta cacgtatacc ggaggaaatc tctaatgatc aaaacaagat
    12661 ttgcaccaag cccaacaggg aatctccaca ttggcggtgc tcggacagcg ttgtttaact
    12721 acctttacgc aaagcatacg ggcggtgttt ttgtgctccg tattgaagac acagatattg
    12781 aacgatcaaa agaagaatac acaaaggata tccttgaagg cttgaagtgg cttggtgtgt
    12841 attgggatga aggcccccac tttcaaaagg agaggatgga tatatacagg caacacgctc
    12901 acagactcct caatgaggga aaggcgtaca aatgctactg ctccactgaa acccttgaag
    12961 agaaacggaa agcagccctc agggaaggga gaaagccaaa ctatgatcgt acctgcagag
    13021 atcttacaga agacagaaag aaccagcctt ttgtcatacg atttaaatct cctgttaacg
    13081 gagaggtttc ttttgaggac cgcatcaggg gaaggattac cttcaaatgt gaagagcttg
    13141 acgatcttgt gatcctcaga accgacaata cacctactta caactttacc gttgtcgtcg
    13201 atgatgccct catgggcata acgagtatca ttcgggggga tgaccacatc aacaataccc
    13261 caagacagat actgatctat gaggccctga actacgaaac ccctgagttt gcccatgttc
    13321 ccctcataca cggcaaagac aaaagccgcc tgagtaagag acacggtgcc acatccttgc
    13381 tggaatacag gaatgatggt tttctgccag aggcattgat gaactatctt gcccgtcttg
    13441 gttgggccca cggagatcag gaggtttttt caagagaaga cttgattgag aagtttgatc
    13501 tcgggaaggt gggtaaatca ccttccatct ttgatatgga taaactgttg tggctcaatg
    13561 gccattatct gaaaacaatg cctgaaggag atattgcagc aaggctcgag ccgttcattg
    13621 aaaatcgtgg gataaaagtc actgacaaga acaagctgat tcctgtagta attaaccttc
    13681 gggatagagc aaagacgctc aaggaaatgg ctgaaatggc tgccttcttt tttaccgatg
    13741 atttcccgta tgatgaaaat gcaaaaaaca agttcctcac tgtttccaca aaaccgatcc
    13801 tggagcgttt tctcaatgag ttcaccagcg tctcctcctt cgacgagaac gaacagcgaa
    13861 agcttttcga aacaatggcc caggatggag gcaagaaact tgtggaggtt atccagcccg
    13921 tcagggtagc tctatgcggc agaacagtaa gccctggaat atttgaggtc gtcagtattc
    13981 tcggtcgtga tagtgtggta aagaggatta aaaaagcaat tgcatcaata gcatgagact
    14041 ccacaggctc gaactttttg gtttcaaatc attctacaac aggacagtct ttcagtttga
    14101 tgaggggatt acatccattg tcggccccaa tggttgcggc aagagcaatg tcgttgatgc
    14161 catggtctgg gcattgggtg agaggggaac caagactctc agggtcaagg acatgggaga
    14221 cgtgatcttc cacggcagca acggaaaaaa gccggtcaat attgcagaag ttaccattga
    14281 tctcaccgat gacggcaaag acatttcagt caagagaagg gtttacaggg atggcaccag
    14341 tgaattcttc ctgaatggtg aggcggtacg tctcaaagat gtgcaggatt tctttcttgg
    14401 cactggcatc ggggtcaaca gctacgccat tgttgaacag ggcaggatag agtatatcat
    14461 ccagatgaag cctcaggaga gacggattgt cgtagaggaa acaagcggta taaccaggtt
    14521 tgaggagaag aaaagggatg ccattgcccg tcttaccgag gtgagttcta atttggagcg
    14581 cgtagaggat atctacacag aggtgaaggc atcttttgaa aaagcagagg tggagtggaa
    14641 cagatggaag atatacaaag aacttgccca taagcagaat gagatagaca agtggatcct
    14701 ccttgatggt tttatgaaag tcaagaagag gatgggcaaa ataatggagc gacatgaaga
    14761 gatagacagg gaatttgccc agaaagaagc agagaagata aaactcagag aagagcttga
    14821 ggccaaagag gatgagttct ctcttgttga taccacaagc agaaaacttg aagtggatat
    14881 caaaggaaaa gaaaaagaca tggaaaaccg tcttctcgaa atcgaatacc tcaaaggcga
    14941 acttgtgcga cttcaagaag agcagaacgg acttgcaaag gccagggaag acctggaaaa
    15001 aacttctgcc atctaccaga aggacattga gtctctgacg gcttcccgca ctgatagtga
    15061 aacactcctg cttgatatag aaaatgaaga gagaggactt caggagagtc tcgaaactca
    15121 gaaaaaaaag gtggcagatc atgaggcgaa gatagaggct gaaagaggcg atctttttgt
    15181 tgctatgagc aagcttacgg aaacaaagaa ccgtatttta gaaattgagc ggcaggcgca
    15241 ggagagaaag aagcgggaag caaaacgagc tgaggagctc gggcggctca acaggcgcct
    15301 cgcacaactc gagttatcgc gttcgactct tcaggaccgt atggatatat caaaggccga
    15361 gagagaccgg gttgccggcg aggaagttgc agcttttgcc gagcgtgaag agattacccg
    15421 ccttatcgat acagaaagaa gagccataga tttattgaaa agcgaaaagc tggccaagga
    15481 ggagttcctg aaacagatgg caagtatcaa gggagtgggc ggaaaaccaa tccctgatac
    15541 gaagcggctt attgaccttg taagggctga agagacccgg gaaaaggctt tggagcgatt
    15601 tttctttaaa gaactcgagt accatgtgct caggggcagt gatgccagcg ctatagctga
    15661 tttggttcaa aaatacgaag gtaacttcat ctttttccca aagaagggaa tattcaggca
    15721 aagctctgat gaggtagagc ttgatgtaaa atggatccgt gacattaacg aaggtctaaa
    15781 gagaatagag gatggcgaag aggggatttt tattaacgat gatgtcttaa tcgactccag
    15841 agggcttgtc ctgagggaga aggaagaaaa aaaggttgac cttaaacaat tccgggaaag
    15901 aaaaaaggct gaaaaagccc tcaaagagat agaggagaac atcgcaaaaa aactcgaagc
    15961 aatgaaagag catcaggatg cccttggtaa gcagggtgca atctggaaaa aatggaaatc
    16021 tcaaaaagat gacattgata aagctcttca tagcctcgaa cgggaatttc ttctgattga
    16081 cgcggagaac aaactcgtta aagaaaggct tgcagaactg aacacagaga tagatctctt
    16141 cgaagaggca ccacaagact ccattgaaag cctgttagag gaacagaagg tacgtgaagg
    16201 ggatagacag aaaagggaag gtaatatggc tgccctgagg caagaactcg atgaagtcaa
    16261 ggccgagtat gaaaacataa gagcacacaa gcaggaggcg gccataagca ttgaacgcca
    16321 tcgtaatgcc ttgaaaaccc ttgatgagga catccggaga aagcttgtcc tgataaaaac
    16381 ggcccaggag gacagagagc gcatcacggc aaagatagag aaagcagcgc ggtcctgtga
    16441 agaggttacg gcaagggccg aaacactgga agaggattat gctcatataa aagaaacctg
    16501 tgaaaagtct gtccaacgct atgagggcct caaagtctct gccggtaacc tccatatgga
    16561 aaaacagatc ctccaggaac gggtcaacac cattataaaa gagatggaac gggtccgtaa
    16621 caggaaggaa gggggcgaga aagagctcgc tgtcctgtca gagaaaagga ataccatagc
    16681 tgaaaggctt gccactgtct atgggattga tacacctgaa aacgttccag ttccccacgg
    16741 ggaagacttt gaaggggaaa gggaaaagat tgttgctgag atagctgagc tgggagatgt
    16801 aaattttcgg gcggaaatgg agtatacaga gcttaaggac aagacggcat ttcttgaaca
    16861 acagaaagaa gacttgagaa atgctatgga ttccctgaaa aaaactattg caaagatcga
    16921 cagcctctcc aaggatatat tcatcgagac ctttgatgtt gtaaataatg cttacaaaaa
    16981 atttgttcag ttgctcttca agggcggaca tgggtatctt gccgtctcac aggatggcag
    17041 cggaatagag atgtatgtcc agcctcccgg gaagaaagtt ttaagaatgg agcagctttc
    17101 aggtggtgaa aaagcgcttg tttccctcgg gtttctcctg gccctcatgg atacaaaacc
    17161 gagccccttt tccttgatgg acgaaatcga tgccccgctg gacgatgcaa acatcatagg
    17221 ccttatggaa ataatcaagg acatcagtaa aaaaacacag atcatcttta tcacccacaa
    17281 ccgtatcacc atggagtgct caaacaccat atacggtgtg accatggagg atgagggagt
    17341 gtccaaagtc gtgtcggtac gactgtgaat cagagaataa gcacgaggca acaggctgac
    17401 ggtttagctt cagttttcct atttcctata accaatcttg cttttttcag taaactggcc
    17461 tgcctgagcg ttacaaaaag attgtataca gtttttaaaa aaatgcaata aaatttgtca
    17521 atgtaatcga atagttgttt gatttcaaat ccagaatggg aaaattaaac taattttttc
    17581 cttgacattt tttttcagac taatatgatt attaaaaagc aaagggggat gggggtttga
    17641 ttaatctaca tgttcgagcc agaacaatca tcaagaaaca acatt
//
LOCUS       DAOOMC010000056        17673 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdus sp. isolate AMR_MDS_2685
            NODE_3327_length_17673_cov_6.285844, whole genome shotgun sequence.
ACCESSION   DAOOMC010000056 DAOOMC010000000
VERSION     DAOOMC010000056.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864760
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdus sp. (wastewater metagenome)
  ORGANISM  Syntrophorhabdus sp.
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae; Syntrophorhabdus.
REFERENCE   1  (bases 1 to 17673)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17673)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,729
            Genes (coding)                    :: 2,708
            CDSs (with protein)               :: 2,708
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 7 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17673
                     /organism="Syntrophorhabdus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3327_length_17673_cov_6.285844"
                     /isolate="AMR_MDS_2685"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2699756"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..61)
                     /locus_tag="PK125_07160"
     rRNA            complement(<1..61)
                     /locus_tag="PK125_07160"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
     gene            368..1213
                     /locus_tag="PK125_07165"
     CDS             368..1213
                     /locus_tag="PK125_07165"
                     /inference="COORDINATES: protein motif:HMM:NF012693.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HPB37923.1"
                     /translation="MADIDLIDVGISEDTGYRSSMEDEHAVYRRPNLQFFSAEVYDGH
                     GGRRASQVAAEMITPHFLHTWNRESEKTLSDQLPLSLFLRDAYVAVDNHLLRESVESG
                     TTGAGLYLIADRFFAVNCGDTKIVIGIDGGVNELTVDHKPDAPEELERIERLGGYVIS
                     FGVSRVMGILAVSRALGDSSLKPFVTAEPRIAEGYLGRENDFAVIACDGVWDVLTPDI
                     VIAITRAAKDAQAAAENIKTTAIDSGSTDNVTVIVLDLRAYVAKLKRGKMEITQIIDG
                     ANQGR"
     gene            1229..2080
                     /locus_tag="PK125_07170"
     CDS             1229..2080
                     /locus_tag="PK125_07170"
                     /inference="COORDINATES: protein motif:HMM:NF015500.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endo alpha-1,4 polygalactosaminidase"
                     /protein_id="HPB37924.1"
                     /translation="MRPMSSLTGKIGCSVKTMENRSPVCPVLTFTAGIIILFLALSSA
                     LLFAQEIWVPPQRTSWQWQLNGRVNQSYDVDMYDIDLFETDPSVVLSLHDQGKRVICY
                     FSAGSLEQWRPDASAFPPSVIGKPLAGWPGERWLDIRKIDIVGPIMAARLDLCREKGF
                     DGVETDNIEGYTNDTGFPLTYEDQLKYNAFLAHEAHARGLSIGLKNDLDQIPDLVHLF
                     DWALNEQCFQFDECHKLMPFIEAGKAVFHVEYKLPAKRFCSAANELNFNSLKKRRSLK
                     AWRKACR"
     gene            2193..2660
                     /locus_tag="PK125_07175"
     CDS             2193..2660
                     /locus_tag="PK125_07175"
                     /inference="COORDINATES: protein motif:HMM:TIGR02777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase ligase N-terminal
                     domain-containing protein"
                     /protein_id="HPB37925.1"
                     /translation="MSSFVVHEHHSKKLHYDLRLEIGGALKSWTIPKGPSMNPKDKRL
                     AVMVDDHPLEYGIFEGIIPEGHYGAGPVIIWDSGTYDSVKQDIIKGKIEFYLRGKKLK
                     GLFVLTRMKGKEKEWLFIKKNDGYSDGNFKTRPELTEERRRLLQEKIPSCHMT"
     gene            2713..3591
                     /locus_tag="PK125_07180"
     CDS             2713..3591
                     /locus_tag="PK125_07180"
                     /inference="COORDINATES: protein motif:HMM:NF013046.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB37926.1"
                     /translation="MKTGVFFHEEFKNKDWPIIGDKFGNFPQVMEKALALPDMQLFEP
                     HPVNEELLYKVHTHGYLQEVKRAWYYKGATLAVGGCVEAGEKIARGELRNALVFSVGA
                     GHHASPDSAWGGTYLSCIGPAIARLTEISGKQRYAILDTDRHHGDGTRAVFEGNSNVL
                     HVCFCYYNNIIEHGTKIDINIDGRIGDKGYLEKVRSEFIPRVRDFNPYIIFHNLGHDT
                     AQGDYGDIGLTREFYLRLVKEVKTVAEEICKGRYIIITHGGSRIDVAEYIFPRIAQIL
                     ADECEIQEVGKENILK"
     gene            complement(3635..4930)
                     /locus_tag="PK125_07185"
     CDS             complement(3635..4930)
                     /locus_tag="PK125_07185"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: protein motif:HMM:NF002223.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HPB37927.1"
                     /translation="MPNIGVVGVQWGDEGKGKIIDILSGYADVIVRFQGGANAGHTIV
                     AGGKKIILHLIPSGILHEGKYCIIGNGVVLDPEVFEKEVNELKGLGFIKDDKKLMVSG
                     LAHVILPYHKKLDALKESKGKKKIGTTGRGIGPAYEDKISRMGVRVIDLLDKKIFSEK
                     VKQNLEVKNFIIKRFYKDEGFQVRTLIKEYGEYRKILKRYTTDTTSFLHKCIEQKKAI
                     LFEGAQGTYLDIDHGTYPYVTSSNTVSGNMFSGSGAPPTSVDYILGICKAYTTRVGEG
                     PFPTELFGEEGESMRRLGDEYGATTGRPRRCGWFDAVIVKRAVKTNGIKGLCMMKLDV
                     LDTFNRIRICTGYRSGKKTVSQPPMSIVDYYRYEPVYEEMDGWKTSTRGITRYDDLPL
                     AAKHYLQRLGELVDARIDVISTGPERESTIILNNPFVKQ"
     gene            complement(5038..5394)
                     /locus_tag="PK125_07190"
     CDS             complement(5038..5394)
                     /locus_tag="PK125_07190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB37928.1"
                     /translation="MFDINCLNRMTINLMAAHMLESVGRKPEPHRLYFLDLVFWSLEQ
                     GHAEVEKSVSETIYAMASWRPQRIMNFLDLLPGQEYNPEGWESAQTPIDLALLVLKDI
                     EDRMFVKFPWYGSFES"
     gene            complement(5521..6357)
                     /locus_tag="PK125_07195"
     CDS             complement(5521..6357)
                     /locus_tag="PK125_07195"
                     /inference="COORDINATES: protein motif:HMM:NF012402.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HPB37929.1"
                     /translation="MASNKNPYIPELVTITNIIEETPNIKSFQVVFNDPERMKTFKFE
                     PGQVGQLSVFGVGESTFVINSPPTRMEYLQFSVMKAGEVTGALHELFPGDQMGLRAPL
                     GNWFPYDMMKGKKILFIGGGIGLAPLRTLILYMLDNRGDYKDITIIYGARTPPDLCYK
                     EDLKEWEARSDVNLILTVDTEYPGWDKRTGFVPTVLNEVAPSPDDTIAITCGPPIMIK
                     FVLQNLAKLNFKDEDIITTLEARMKCGIGICGRCNLGSQYICKDGPVFSLAQLRDFPG
                     VV"
     gene            complement(6347..7411)
                     /locus_tag="PK125_07200"
     CDS             complement(6347..7411)
                     /locus_tag="PK125_07200"
                     /inference="COORDINATES: protein motif:HMM:NF024581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HPB37930.1"
                     /translation="MADTGFIPKEKWGAFIEGLAAKAQVWVPCLDGDVVIFKPYSKDQ
                     TLCFERPANSPPKGVIFPQSDTLFAFNFSKDPENPQKVMVELEEKLDFPKTIIFGSRP
                     CDAKGFLIYDRPYMQTDTPDPYYKGRREQTAIVTMSCQSTYAGCFCTAVGGGPADKEG
                     SDVLITELDNGYFIEAITDKGKEILQQAAAESGAGHEAEALKKQEAVRARVKDPFCAN
                     GKPEISKDLFESDEFWEEELSKCVSCGACTYLCPTCYCFNITDETTFEKGERIRSWDG
                     CMYNHFTLEASGHNPRPTKFQRFKNRVGHKFLFYPEKYNGVIACCGCGRCIRFCPMSV
                     DISEIVSKLQKQTGSASNGK"
     gene            complement(7411..8364)
                     /locus_tag="PK125_07205"
     CDS             complement(7411..8364)
                     /locus_tag="PK125_07205"
                     /inference="COORDINATES: protein motif:HMM:NF016328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HPB37931.1"
                     /translation="MSLDKLKQIITDVMKEVDVVIGYKQGFDPLHATPCFITKPEQVD
                     QVIWNPLCVQNLASYLTSLKKKVGVIVKGCDSRTIVQFMQEGLIDRSKVVVIGIPCTG
                     VVSVRKVLEKVKHEPIEEVKFSDDGTVIVKTPSGETKMALSEVAPDKCETCQYPTPVL
                     YDHLAGEPITSTKDPQSVFKDIEEFDKKSLEEKKAYWEKEFDRCIRCYACRNACPMCV
                     CQDSCIAESREPHWVSQKSNLTEKTMFHMIHALHLAGRCVECGECERACPMGIPVNKL
                     KRKINSDMKVLFGYEPGVKPEDKPPMFTFSVEEKNIEEHKL"
     gene            complement(8361..8792)
                     /locus_tag="PK125_07210"
     CDS             complement(8361..8792)
                     /locus_tag="PK125_07210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013840288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase iron-sulfur subunit"
                     /protein_id="HPB37932.1"
                     /translation="MSEVGPKELRIVGFLCNWCSYGGADTAGVSRFKQPTDLRIIRVP
                     CSGRVDPLFVVKALLNGADGVLVSGCHPRDCHYSDGNFYARRRLEMLKRLLPFLGIDD
                     KRFHYTWISASEGQRWQQTVTDFTKQIHKLGPLHSGKEAQQ"
     gene            complement(8780..10741)
                     /locus_tag="PK125_07215"
     CDS             complement(8780..10741)
                     /locus_tag="PK125_07215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013120810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoB--CoM heterodisulfide reductase iron-sulfur
                     subunit A family protein"
                     /protein_id="HPB37933.1"
                     /translation="MRVGVFICHCGSNIAGTVDVARAAAELRKVPGVAFATNYMYTCS
                     EPGQDEIKQAIKRENLDRIVVAACSPRMHELTFRRTIEKAGLNRYFLEMANIREHISW
                     VGLDKEANTIKAIETIKMAVAKVIQNKPLYSSSFKINKRVLVIGGGVAGMQAALDCGD
                     GGLEVVLVEKSPSVGGMMARLDKTFPTIDCSICILGPKMVDVAQHDKIKLYAYSEIED
                     IKGYVGNYEVKIRKKATYVDWTKCTGCGLCMEKCPSKNSYDYFNFGVAPTKAINIPFP
                     QAIPKKAKIDPDFCRQFTKGKCGVCAKICPTGAINYEMQDEIVTEEVGAIVVASGYGL
                     IDMDKFPEYGGGRYPDVITGIQYERLLNASGPTEGHIVRPSDHTEPKTVVFVSCAGSR
                     DKSLGIPYCSNFCCMYIAKQAILTKDHIPDSQSYVFYMDIRSPGKGYDEFTRRAQEDY
                     GAKYVRGRVSRIYPKGKKLVVVGADTLLGTQVEVEADLVVLATAVTAAPGAAQLAEKL
                     HISYDTFGFYVESHPKLRPVETNTSGVFLAGAAQGPKDIPASVGQGSAAAAKVLALFS
                     KDMLESDPAVAKVNENTCVGCLKCKLTCPFGAIQEKELRGGKIVASVIETVCAGCGVC
                     TSTCPCGAIQLSHFTDNQLLAEVNAICQK"
     gene            complement(10745..11740)
                     /locus_tag="PK125_07220"
     CDS             complement(10745..11740)
                     /locus_tag="PK125_07220"
                     /inference="COORDINATES: protein motif:HMM:NF014774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoB--CoM heterodisulfide reductase iron-sulfur
                     subunit B family protein"
                     /protein_id="HPB37934.1"
                     /translation="MAEKEYAYYTGCSLEGTAVEYDMSMRQVFKALGVGIIEPEDWSC
                     CGSTPAHTVDRVFAAALAARNLTIIEKMGTGIVTVPCPSCLSVLKRAHLGMTQDESFK
                     NEVNELLDEPYEGGVVPKSTLQVMLEDVGLDAIASKVTHALPGLVVAPYYGCILTRPP
                     EIAQFDDPENPVSMDKILEAVGVKVADYAFKLECCGAAFGVPKRDMVNRLTYRVLSMA
                     LEAGANCIAVACPLCQQNLDLRQGQVNSTMGSSFNLPILYFSQIVGLTYGLSPKELGM
                     DKLVVSADDIVRSRITIEELAQRKANAKAAEAEAKAAEKLKKAKAKEEDTKTEET"
     gene            complement(11733..12299)
                     /locus_tag="PK125_07225"
     CDS             complement(11733..12299)
                     /locus_tag="PK125_07225"
                     /inference="COORDINATES: protein motif:HMM:NF024924.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HPB37935.1"
                     /translation="MDTLNLTKSLNLSFIEQVSKESGQNPSLCYQCGNCTAGCPYTHF
                     FDYPVSQIMRLLQAGQRDTILSSKAIWLCATCETCTTRCPCEIDVAMIMDTLRIIARR
                     ENKVSEKDVKLFYDSFLNSMKEHGRLFEVGTLLSYNLRSGNFFADADLGPKVLEKGKI
                     HFLPRKIKGRDKVAKIFTRFQEKAKKHG"
     gene            13091..13738
                     /locus_tag="PK125_07230"
     CDS             13091..13738
                     /locus_tag="PK125_07230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB37936.1"
                     /translation="MPILFTMNWDIIPGNEQEYSEFIAKTFIPEATAMGILPVGGYYV
                     EVGFGPRVIGVNTCENQQALANVVTTQRYRDLILKLKTMVYNFRTAVLEPTGHFKTEQ
                     YFIQKGVWKFNQYYDLRPGKKDGYRDYVLNVHLPTMDKIDYVEVTGGWNVTFGGVSEI
                     IAEFTVKDPVDIGRLLNNDDFRRITLGLRTDYVWNYQSRILRCTERFDEPKWFKL"
     gene            13765..15606
                     /locus_tag="PK125_07235"
     CDS             13765..15606
                     /locus_tag="PK125_07235"
                     /inference="COORDINATES: protein motif:HMM:NF006152.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-utilizing enzyme"
                     /protein_id="HPB37937.1"
                     /translation="MAIKFMDPHDVPQIPGTEGWERMYPYQYHFTKDDPERAAYESNQ
                     IWYYDGLHYPEPHYPFDLIWDEAWYLALSQFNTRHWLVPPANGIDHRIVNGYVYISPV
                     GVENPEDIPKRVPHFQERAGFYFQNWNDLYEKNWKKKVGNVIEQLEAINFTDLPEMED
                     ISVIKEGIGKGSGYEFLKKYDKLIDLGLESWQYHFEFLNLSYAVQVNFFQTMNQLFPG
                     IPMATLTKMSAGFDPILFRPDAELIRLAKLALEIGCEELILKPLKADELMKALAEDSI
                     GKIWLQELERIRYPWFHISVGTGWYHTHISWNDNLEIPFDNIRTNIRAIKAGKTLGRP
                     TDQILKERDRIATEYRALLKTDADREAFDQGLNLARLVLPYAEDHNFYIEHWFHTLFW
                     GKVRQIGQVLTNAGFFGDADDIWYLRRDEIKQALWDLCTAWATGVKPRGPSYWPKEID
                     WRKGVYEKFKEWTPPAALGTPPEVVTEPFTIVLWGVTSDVLKTWLKGGEEDGGDPNEL
                     QGSPGSSGIAEGKARVLKSVDQLVDLKEGEILVATTTSPSWAPAFVKIAGAITDVGGP
                     MCHAAIVCREYGLPTVVGTGKGTQFIKTGDLIRMDGDTGKVTILQRA"
     gene            15685..16749
                     /locus_tag="PK125_07240"
     CDS             15685..16749
                     /locus_tag="PK125_07240"
                     /inference="COORDINATES: protein motif:HMM:NF013491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HPB37938.1"
                     /translation="MGDKDKRYILWYDEMEGADFPIVGKKNSNLGEMIKAGIPVSHGF
                     AITIYANDQFLVLTGIKEEIEKQITSLGKVTYETAKQASAIAMNLIEKAKMPEIIERE
                     ILDNYKKLCDQTCMSACPVAVRSSGAVSMPGQMETYLNICGENDLIDYVKKCWASAYN
                     TEAIMYRMNKGMPFLFNIGVGIPKMVNSRVSGIIFTINPINGDPSKISIDASYGLGEA
                     VVSGLVTPDTYLIDKVTFDIIKTTVGSKETMCVYKDEGSDIVQKDVPEEKRGVSSLSQ
                     QEIKELARIGKLIEQYYGKAYDIEFGIDADYPFPENIIILQVRPESVWSKKEVEAKVE
                     AKKDPMDRLLSTLLAGVKIK"
     gene            16947..17666
                     /locus_tag="PK125_07245"
     CDS             16947..17666
                     /locus_tag="PK125_07245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB37939.1"
                     /translation="MEQQGGLKQPALGIVGLFVVVFIAFGITTWFKPETFIPWAGELA
                     MCLIPTAIIMGMVWQGNYPPPAVSLAQPLKSTYLLFLNMLVGALVAGYSIKTVGVFVT
                     PPTPPLIFFTIMTVIMTFWCVVVWRCWPAAGIKDNHPAFVGFGTLIVSYAVAYILWKI
                     FFNFDFMKGAPFYDAVALPSGAFFAFWSLGFFLTCLAVILAWVELDFWPLSSIPAKVP
                     AFGTQPLWGTVVSMTVFLFKQ"
BASE COUNT         4764 a         4161 c         3939 g         4809 t
ORIGIN      
        1 cttgcatgtg ttaggcacgc cgccagcgtt cgttctgagc caggatcaaa ctctcagatc
       61 aaacttaaaa attgttcatt acttgaacga ggacctgcta tttttctgtt ttcaaagaac
      121 actaacccac ttgcgttaaa gcagtactaa ttatacctat tttttcgcaa ttgtcaagcg
      181 attacacaaa tatttttttc tcctgtaata tgggcacgtt gagtcccggc gttgcccctc
      241 caaaccttta cccttcaagg gatcagcaga ttttcaggag aaacattagt atccctttct
      301 gtgttttttt tattcagaca taggctcacc aattgaatat ggagtccttc cagattataa
      361 tggaggggtg gcagatattg atcttattga tgtcggcata agtgaggata ccggttaccg
      421 aagttcaatg gaggatgaac atgcggtata ccgcagaccg aacctccagt ttttcagtgc
      481 cgaggtgtat gatggccatg gcggaaggag ggcttcccag gtagccgcag agatgattac
      541 ccctcacttt ctccatacat ggaaccggga atcggagaag actctcagcg accaactacc
      601 gttaagcctg tttctaagag acgcctatgt tgccgtcgac aaccatcttc tccgggagag
      661 tgtcgaaagc ggtacgaccg gtgccggcct gtacctgata gcggatcgtt tttttgcagt
      721 caactgcggc gacacaaaga tcgttattgg catagacgga ggtgttaacg agctgacagt
      781 agaccacaag cccgatgcgc ccgaggagct tgagcgaatt gagcggctcg gtggctacgt
      841 catctctttc ggggtatccc gcgttatggg aattttggct gtaagcaggg ccttggggga
      901 ttcatcgctc aaacccttcg taacggcaga gccgagaata gctgagggct atttggggcg
      961 tgaaaacgat ttcgccgtca tcgcctgcga tggcgtgtgg gatgttctca ctcccgacat
     1021 agtcatcgcc ataacaagag ctgccaaaga cgcccaggct gccgcggaaa acattaagac
     1081 cacggctatc gactccggaa gcactgacaa cgttacggtc atcgtgctcg atttgcgggc
     1141 ctatgtggcc aagctgaaac gggggaaaat ggaaatcacg cagattatcg atggggcaaa
     1201 ccagggaaga taaaagaaga ggaagacaat gcgacctatg tcatcgctca cgggaaaaat
     1261 tgggtgtagt gtaaaaacaa tggaaaaccg ttcgcctgtt tgtcctgtcc tgacatttac
     1321 agccgggatt attattctat tccttgcttt gtcttcagcc cttctcttcg cgcaggaaat
     1381 atgggttcct cctcagcgaa cgagctggca gtggcagctc aatggcagag tgaatcagtc
     1441 ctatgacgtg gatatgtacg acatagacct gttcgaaacc gatccatccg tcgtgctctc
     1501 cctccacgat caaggaaaaa gggtgatatg ttatttcagc gctggaagtt tggagcagtg
     1561 gagacccgat gcttccgcat ttcctccctc agtcatcggc aaacctcttg caggctggcc
     1621 tggcgagagg tggctcgaca tcagaaagat tgatattgtc ggacccatta tggcggctcg
     1681 ccttgacctg tgccgagaaa agggttttga tggtgtggag acggacaata tagagggtta
     1741 cacaaacgat accggttttc cattgacata tgaggaccag ctgaaataca atgcctttct
     1801 tgctcatgag gctcacgcac ggggcctctc cataggacta aaaaacgatt tggatcagat
     1861 tcccgacctt gttcatcttt ttgattgggc cctcaatgaa cagtgttttc aattcgacga
     1921 gtgtcacaaa ctgatgccct tcatcgaggc ggggaaagcg gtctttcatg tggagtacaa
     1981 gctccctgcg aagaggtttt gctccgcggc aaatgagctc aatttcaatt ctttgaagaa
     2041 acggaggagc cttaaggcct ggcgaaaagc gtgccggtag gtgattcaca ggctcgcgca
     2101 gacaggattc gccagacttt gtcattgaag atgggctacc ccatgcccaa aggctcttaa
     2161 tatctcttcg caaatatggt aatataaaag tcatgtcctc cttcgtcgtc cacgaacatc
     2221 attcgaagaa actccactat gacttgaggt tggagatcgg cggtgctctc aaatcatgga
     2281 cgatcccaaa gggaccttcg atgaacccca aggacaagag actcgccgta atggtggatg
     2341 accatcccct cgaatatggc atattcgagg ggattatacc ggagggccat tacggtgccg
     2401 gcccggtcat tatttgggac tccgggacat acgattcggt caagcaagat attatcaaag
     2461 gcaagataga attttacctc cggggaaaaa agttgaaggg tctcttcgtt ttgaccagaa
     2521 tgaaggggaa ggaaaaggag tggctcttca taaagaaaaa cgatggatac agtgatggga
     2581 atttcaagac aagaccggaa cttacggaag agaggcggcg tctcttgcaa gagaaaatcc
     2641 cgtcatgcca tatgacatga aagaatcagg gtcgcgcctt caccgtactc agcgttcagg
     2701 agtaatgatt ggatgaagac tggcgtattt tttcacgagg aattcaagaa caaggactgg
     2761 cctatcatag gggataaatt cggtaatttc ccccaggtga tggaaaaagc ccttgccctg
     2821 ccagatatgc agctttttga gccacacccc gtcaatgaag aacttctgta taaagttcat
     2881 acccatggtt accttcagga ggtaaaacgc gcatggtatt acaagggcgc aacccttgca
     2941 gttggtggat gtgtggaagc cggagaaaaa attgctcggg gcgagctgag aaacgctctt
     3001 gtcttttcag taggcgcagg acatcatgct tcgcctgact cagcctgggg cggaacttac
     3061 ctctcatgca taggaccagc cattgcaaga ctaacggaaa tatcgggcaa gcagcgttat
     3121 gccatccttg acacagatag gcatcatggt gatgggacac gggctgtttt tgagggtaat
     3181 tcgaatgttc tgcatgtctg cttctgttat tacaacaaca tcattgaaca tggaacaaag
     3241 atcgacatca acatagatgg caggatagga gacaaaggat atttagagaa agttcggtca
     3301 gaatttattc ccagggttcg tgatttcaat ccttacataa tttttcacaa tctcggtcat
     3361 gacacagccc aaggcgatta tggcgatatt ggtctgacga gagaattcta tctccgtctt
     3421 gtcaaagaag taaaaactgt tgccgaagag atatgcaagg gaagatacat tatcatcacc
     3481 cacggtggct cccgcattga tgtggcagaa tatatttttc ccaggatagc ccagatacta
     3541 gcggacgagt gtgaaatcca agaggttgga aaagagaata tcttaaaatg aatccttact
     3601 tatctattac gttctatctt tcccttctca ccttttactg cttcacaaaa ggattattca
     3661 aaattattgt gctttcacgt tctgggccgg ttgaaataac atcaatcctt gcatcaacca
     3721 attctcccaa tcgctggaga tagtgtttcg cagccaatgg aagatcgtca taccttgtaa
     3781 ttcctctggt tgatgtcttc catccatcca tttcttcata tacaggctca taccggtaat
     3841 aatcgacaat actcatgggg ggctgggaaa ctgttttctt tccagaacga tatccagtac
     3901 aaatccttat tctattaaag gtatcgagca cgtcaagctt catcatgcat aaccctttga
     3961 taccgtttgt ttttacagcc ctttttacga tcaccgcatc aaaccatccg caacgcctag
     4021 gcctgcccgt tgtagcaccg tattcgtctc caagtctcct cattgactcg ccttcctctc
     4081 cgaacaactc ggtaggaaat gggccttctc ctacccttgt agtatatgcc ttgcaaatcc
     4141 ctaaaatgta gtctactgat gttggcggag cacctgagcc tgagaacata ttcccagaaa
     4201 cagtatttga tgatgtaacg taaggatatg ttccatgatc gatgtcgaga tatgttcctt
     4261 gcgcaccttc gaaaagaatg gctttttttt gttcgataca cttatggaga aaactcgttg
     4321 tgtctgtggt atatcttttc aatatctttc ggtattcacc atattctttg atgagtgtcc
     4381 tcacctgaaa accctcgtct ttgtaaaatc ttttaataat aaaattttta acctcgaggt
     4441 tctgcttcac tttctctgag aatatcttct tatcaagaag atcgataacg cgaacgccca
     4501 ttctgctgat cttgtcttca tacgcaggtc caatgccgcg cccagtcgtg ccaattttct
     4561 tttttccttt cgattctttg agtgcatcaa gttttttgtg ataaggcaga ataacatggg
     4621 ccaggccact caccatgagt tttttatcat cctttatgaa tccgagtccc tttagttcat
     4681 taacctcttt ttcaaaaact tcgggatcaa gaacaacacc atttcctata atacagtatt
     4741 taccttcgtg aagtatccct gaaggaatga gatgaagaat aattttttta ccacctgcaa
     4801 caattgtatg acccgcattt gctccaccct gaaaacggac aatgacatca gcatagccac
     4861 tcaggatatc aatgattttt ccttttccct catcacccca ctgaacccct accacaccta
     4921 tgtttggcat taaaaaacct cactttgttt aattgtgcaa aacacatatt tctaatacac
     4981 tgtgtgaata aaatcaagaa aactcggact caataagaca ccactgttga ttctggatca
     5041 cgattcaaag cttccatacc atggaaactt cacaaacatc ctgtcctcta tgtcttttaa
     5101 caccagtaaa gccaggtcaa ttggtgtttg tgctgactcc cacccttccg gattatactc
     5161 ctggcctgga aggagatcaa gaaagttcat aatcctctga ggacgccatg aagccattgc
     5221 atatatagtc tcggacactg atttttcgac ctctgcatgt ccttgttcga gactccagaa
     5281 taccaggtca agaaaatata aacgatgagg ctcgggcttc cgtcccacag attcgagcat
     5341 atgggcagcc ataaggttta ttgtcatgcg gtttagacag ttgatatcaa acataggctc
     5401 tttgtgaaat tgccaaacct ctgcgcggaa atgtgttttt gtgtacaggg acgcagccac
     5461 gcacccatgg ccgtatggac attttgcagg aagcaacgca atataaggat tgggctattt
     5521 ttagacaact cccgggaaat cccgcaactg tgccaatgag aatactggcc cgtccttgca
     5581 gatgtattga gaaccgaggt tacaacggcc acagattcct atcccgcact tcatcctggc
     5641 ctcaagggtt gtaatgatat cctcatcctt gaaattcaac ttggcaagat tctggagtac
     5701 aaacttgatc attataggtg gtccacaggt tatggctatt gtatcatcag gggatggagc
     5761 cacttcgttg agaactgtgg gcacaaagcc tgttcgttta tcccaccccg ggtattctgt
     5821 atccactgta aggatgagat tcacatccga gcgtgcttcc cattccttaa gatcttcttt
     5881 atagcagaga tcagggggtg tcctggctcc gtaaatgatt gtgatatcct tgtagtcacc
     5941 tctattatcc agcatataga gaattaaggt ccgaaggggc gcgagcccta tgccaccgcc
     6001 gatgaaaagg attttcttgc ccttcatcat gtcatagggg aaccaatttc ccagaggtgc
     6061 cctgagaccc atttgatccc ccggaaacag ttcatgaagg gctccggtca cttccccagc
     6121 cttcattaca ctgaattgga ggtattccat ccgtgtcggg ggtgaattga ttacgaaagt
     6181 agactcccct accccgaaca ctgaaagctg cccaacctgg ccaggttcaa acttgaatgt
     6241 cttcatcctc tcaggatcgt tgaaaacaac ttgaaaggac tttatgtttg gtgtttcctc
     6301 aattatgttc gtaatggtaa ccagttcagg tatatagggg ttcttgttac ttgccattgc
     6361 ttgcgctccc tgtctgcttc tgtaattttg atacaatttc actgatgtct accgacattg
     6421 ggcaaaaccg gatacaccgt ccgcacccgc agcaggcaat cacaccgtta tatttttcag
     6481 gataaaagag gaacttgtgt cctaccctgt ttttaaaccg ttgaaatttt gttggtctgg
     6541 ggttgtgacc gcttgcctca agggtaaagt ggttatacat acacccgtcc caacttctga
     6601 tacgctcacc tttttcgaaa gtcgtctcat ccgtaatatt aaaacaatag catgtggggc
     6661 agagatacgt gcaggcaccg cagctcacac atttggaaag ctcctcttcc cagaactcgt
     6721 cactttcaaa tagatccttc gatatttcag gttttccatt tgcacagaat gggtccttga
     6781 ccctggcacg aactgcctcc tgttttttca gggcttcagc ttcatgtcct gcaccgcttt
     6841 cggctgcagc ctgctgcaaa atctctttac ctttatctgt aatggcttcg ataaaatacc
     6901 cattatccaa ttccgttatc agtacatctg atccttcctt gtccgccggc ccccctccaa
     6961 ccgcggtaca gaaacatcct gcgtaagtcg attgacagga catagtgacg atggcggtct
     7021 gctctctcct gcctttataa taaggatcgg gagtgtctgt ctgcatgtaa ggccggtcat
     7081 atatcaagaa gccttttgca tcgcatggtc gagaaccgaa gataatggtt ttgggaaaat
     7141 cgagtttttc ctcaagctca accatgacct tctgaggatt ctccgggtct ttgctaaagt
     7201 tgaaggcaaa aagtgtatcg ctctgaggga agataacacc ttttggaggg ctgttggccg
     7261 gtctttcaaa acatagcgtc tgatctttgc tgtaaggttt aaaaataacg acatcgccat
     7321 caaggcacgg gacccaaacc tgggcttttg ccgccagccc ctcaataaac gcaccccatt
     7381 tttcttttgg gataaatcct gtgtctgcca ttagagtttg tgctcctcta tattcttttc
     7441 ttctacgctg aacgtaaaca taggaggctt gtcctccggc ttcactcctg gctcatagcc
     7501 aaaaagcact ttcatgtcgc tgttgatttt ccttttcaac ttatttacgg gaatacccat
     7561 gggacatgcc cgctcacact ctccacactc aacacagcgt cctgcaaggt ggagggcatg
     7621 gatcatatgg aacatcgttt tctcggtaag gttcgacttc tggctcaccc agtgaggttc
     7681 acgactttct gcaatgcagc tatcctgaca cacgcacatg gggcaagcat ttcgacaagc
     7741 atagcagcgg atacaccggt cgaattcctt ttcccagtat gccttcttct cttccagaga
     7801 tttcttgtcg aattcttcaa tatccttgaa tacgctttgg gggtctttgg ttgatgtaat
     7861 aggctcacct gccagatgat cgtaaagaac aggtgttggg tactggcacg tttcacattt
     7921 atctggcgca acctctgaaa gggccatctt tgtctcgcct gatggggtct tcacaattac
     7981 cgtaccgtca tcactgaatt ttacctcctc aatgggttca tgtttaacct tctcaaggac
     8041 ctttctcacg ctgacgactc cggtacaagg tatccctata acaacaacct tgctcctgtc
     8101 aataagcccc tcctgcataa actgaactat agtcctgctg tcacagcctt ttacaatcac
     8161 acctaccttc tttttcagag acgtaaggta gctggcaaga ttttgaacac acaacgggtt
     8221 ccagataacc tgatcaacct gttcgggttt cgtaataaaa cacggggtgg catggagtgg
     8281 atcaaagccc tgcttatatc ctataaccac atctacttct ttcattacgt ctgttataat
     8341 ttgtttcaat ttatcaagac tcactgctgt gcctccttgc cggaatggag cggacctagc
     8401 ttatgtatct gcttggtaaa gtccgtaacg gtctgttgcc accgctgtcc ttcggaggct
     8461 gaaatccatg tataatggaa tcgtttgtcg tctataccca gaaagggtaa gagccgcttt
     8521 aacatctcga gtcttctccg tgcgtaaaaa ttaccatccg agtaatgaca gtctcgggga
     8581 tgacaacccg acacaagcac cccgtcagct ccattaagaa gggccttgac tacaaaaagt
     8641 ggatcgacac ggcctgagca gggaaccctg atgatcctca gatcagtggg ctgcttaaaa
     8701 cggcttaccc cggcagtgtc tgctccacca tacgagcacc agttgcagag aaaaccgact
     8761 attctaagct ctttggggcc tacttctgac atatggcatt gacctccgct agcaattgat
     8821 tatctgtaaa atgggatagc tgaatggcgc cgcatggaca ggtggaagta cagacgccgc
     8881 aacctgcgca aaccgtttca atcacgcttg caacgatctt accaccgcgc agctcttttt
     8941 cctgtatggc gccaaaagga caggttaatt tacatttaag gcatccaacg catgtattct
     9001 cattcacttt tgccacagcc ggatcagact caagcatatc ttttgagaaa agggctagaa
     9061 ctttggccgc tgccgcgcta ccctgcccta ccgatgcggg aatgtcttta ggcccttgcg
     9121 ctgcacctgc tagaaaaaca ccggaggtgt tcgtctccac aggtcgtagc ttcggatggc
     9181 tttccacata gaaaccaaag gtatcataag atatatggag cttctcagca agctgtgccg
     9241 caccaggtgc ggcagtcact gctgttgcaa gcaccacaag gtctgcttcc acctccacct
     9301 gggtaccgag gagagtgtct gcccctacaa ctacgagttt tttccctttg gggtatatac
     9361 gggaaaccct ccctctgaca tattttgccc cataatcctc ctgagcccga cgggtaaatt
     9421 catcgtatcc tttgcctggt gaccgtatat ccatatagaa tacgtaggat tgagaatcgg
     9481 gaatatgatc cttggtcaga attgcctgtt ttgcaatgta catacagcag aaatttgaac
     9541 aatacggtat accgagagat ttatccctcg acccagcaca cgagacaaaa acaacagttt
     9601 tcggctcagt gtggtctgat ggcctcacaa tgtgcccctc tgtgggaccg gaggcgttca
     9661 gaagcctttc gtattggatg ccagtaatga catcagggta acggccccca ccatactcgg
     9721 ggaatttatc catatcaata agcccatagc ctgatgctac cactatcgcc ccaacttctt
     9781 ccgttacaat ttcatcctgc atctcgtagt tgattgcccc tgtggggcat atcttggcac
     9841 agacaccgca tttacctttt gtaaactgtc tgcagaaatc aggatcaatc tttgcttttt
     9901 taggaatagc ctggggaaag ggtatattga tggctttggt gggtgcaaca ccaaagttga
     9961 aataatcata ggagttcttt gatgggcact tctccataca tagtccacag ccagtgcatt
    10021 tggtccagtc cacgtaggtt gccttctttc tgatcttaac ctcatagtta cccacatacc
    10081 cttttatatc ctcaatctcc gaataggcat aaagcttgat tttgtcatgc tgggcaacat
    10141 ccaccatctt tggacccaag atacaaatgg agcagtctat tgtgggaaac gtcttatcca
    10201 gacgggccat catacctccc acacttgggc tcttctcaac aagcactacc tcgaggccgc
    10261 cgtcaccaca atccagggct gcctgcatgc cagcaactcc tcccccaatt acaaggactc
    10321 tcttgttgat cttgaatgac gaggagtaga gtggtttgtt ttggatgacc tttgcaacgg
    10381 ccatttttat tgtttcaatc gccttgattg tgtttgcctc tttatcgaga cctacccatg
    10441 agatatgctc tcttatattg gccatttcaa gaaagtaacg gtttaggcca gccttctcaa
    10501 tggtccgtcg gaatgtcaac tcatgcatcc tcggagaaca tgcggccacg acgatcctgt
    10561 caagattctc cctcttgatg gcttgcttga tctcgtcctg accaggctct gaacatgtat
    10621 acatatagtt cgtggcaaag gccacgccag gtacctttct caactccgct gctgcccgtg
    10681 caacatctac tgtacctgca atattactac cgcaatgaca aataaatacg ccaacccgca
    10741 tggattacgt ctcctccgtc tttgtgtctt cttctttggc tttagccttc ttgagctttt
    10801 ctgctgcttt cgcttcagcc tcggctgctt tggcgttagc ctttctctgt gccaattctt
    10861 ctatagtaat ccgtgagcgg acaatatcgt cggcgctcac aaccagtttg tccatgccaa
    10921 gttcttttgg tgaaaggcca taggtcaagc ctacaatctg agaaaaatac agaatgggaa
    10981 ggttgaagga tgaacccata gtactattca cttggccctg gcgcaagtca aggttttgct
    11041 ggcagagggg acaggccacc gctatacaat ttgccccagc ttcaagggcc atagagagca
    11101 cccgataggt gagtcgattg accatatccc tcttgggcac gccaaatgct gcaccgcaac
    11161 attccaactt aaacgcatag tcagcaactt taacaccaac tgcctcaaga atcttatcca
    11221 tagacacggg attttccgga tcgtcaaact gggcgatctc ggggggtctc gtcagaatgc
    11281 atccatagta tggagccact acgagaccag gcaaggcatg agttactttg gaggcgatag
    11341 catcgagccc aacatcttca agcatgactt gtaatgttga ttttggcacc acaccgcctt
    11401 catagggctc atccagaagt tcgtttacct cgtttttaaa agactcatcc tgagtcatgc
    11461 ccagatgggc tctcttaaga acagaaaggc aggacggaca tggtacagtc actataccgg
    11521 ttcccatctt ctctattatg gtcaggtttc gagctgccaa ggcagctgca aaaacacgat
    11581 ccactgtgtg agccggcgtc gacccacaac aactccagtc ttctggctct atgataccca
    11641 ctccgagagc cttgaaaacc tgtcgcatag acatatcgta ttcaactgcc gtcccttcta
    11701 atgaacaacc agtgtaatat gcgtactctt tttcagccat gtttttttgc cttctcctgg
    11761 aatcttgtaa agatttttgc tactttatcc cttcctttga tcttccttgg caagaaatgg
    11821 attttcccct tttccaggac cttagggccc agatccgcat ctgcaaagaa attccctgac
    11881 ctgagattat atgagaggag cgttcccacc tcaaaaagcc tcccgtgttc cttcatagaa
    11941 ttgaggaacg agtcataaaa aagcttaaca tctttttccg acaccttgtt ttcacgacgt
    12001 gcaattattc ttaacgtatc catgatcata gccacatcaa tctcgcaagg acaacgggtg
    12061 gtacaggttt cacacgttgc acacagccag attgctttgg aggaaagtat tgtgtcgcgc
    12121 tgtccagcct gaaggagtct cataatctgg cttacaggat aatcgaagaa atgggtgtat
    12181 gggcaaccag ccgtacagtt gccacactga taacaaaggg agggattctg cccactttct
    12241 ttactgacct gttcgataaa actcaaattc aggcttttcg tgaggttcaa agtatccata
    12301 tattcacctt aaaagttaac ttccttatca atgcaaatga attttatcac aagtgatttt
    12361 ccttggcaat aattatttgt atacgtcacc attatatatc tgccatagcc acattcggtg
    12421 aatcttatcg taattaactg tttttgttga taaaatccat acattggtcc ttacgagtga
    12481 taacgacagc actatatatt ctgtaaaaat taacgagtgt cagtgcaaaa accatttcaa
    12541 ccagacaaat ttatgaaaag tgcattccag aattactgta ttttccatag agatatggaa
    12601 actaaatata catatttcat tttttcctta ttcattcttc attgatgaat ggtcagcaaa
    12661 taccatggca gaataactct gagatggtca ccatatttca aacaaacagg cccacaaact
    12721 ttctttactg atcggttttt tctcttatga tgcaggaaaa gttctaatac cggcttggta
    12781 atgatgaaga acctcttgct cgtttacaaa atgttttcga atagttactg atttttctgt
    12841 ccttttgttc cactcgtcaa aagagcaaat taggtaatag gcagcaggtc ataagaaatt
    12901 cttatatatc ataagattaa attagttgac aagaaaactt attacgctag aatgcaataa
    12961 tagcttgtga aatttgcgat ttcaaataaa atacttccaa acgaacttag aagatcatca
    13021 ttttccatga tgattataaa taatagatta tgttaagatt ataactaaac caaatcaaag
    13081 gaggtctttc atgcctattc tctttaccat gaattgggac atcataccgg gaaatgaaca
    13141 ggagtactct gagttcattg caaaaacttt catacctgag gccactgcaa tgggtatcct
    13201 ccctgtgggc ggatattatg tggaagtcgg atttggtccc agagttattg gagttaacac
    13261 ctgcgagaac caacaggcgc ttgcaaatgt tgtcacaacc caaagataca gagaccttat
    13321 cctcaaactt aaaacaatgg tttacaattt ccggacagcg gtgctggagc caacagggca
    13381 tttcaaaact gagcagtatt ttattcaaaa aggagtatgg aaatttaacc agtactatga
    13441 tcttcgccct ggcaaaaaag acgggtacag ggattatgtc ctgaacgttc acctgccgac
    13501 aatggacaaa atagattatg ttgaagtgac aggtggctgg aacgtaacat tcggcggagt
    13561 aagtgaaatc atcgccgaat tcacagtaaa ggatcccgta gatatcggtc gcctcctgaa
    13621 taatgacgat tttaggagga ttactctggg gttaaggaca gactatgtct ggaattatca
    13681 aagccgcata ctccgttgca cggagaggtt tgatgaacca aagtggttca agctctaaaa
    13741 atataataaa aggggaggct gtttatggct atcaagttta tggatccaca cgatgttcct
    13801 cagattcctg gcacagaagg ctgggaaagg atgtacccgt atcaatacca tttcaccaaa
    13861 gatgaccctg agagagcagc atacgaaagt aatcagatat ggtactacga tggattgcac
    13921 taccctgaac cccattatcc tttcgatctc atctgggatg aggcatggta tcttgcactt
    13981 tcacagttta atacacgcca ttggctggtt cctcctgcca atggcattga ccaccggatc
    14041 gtaaacggtt acgtgtacat cagccctgtt ggggtggaaa accctgaaga tatccccaaa
    14101 agagtgcctc actttcagga acgtgcaggg ttttatttcc agaactggaa tgatctttat
    14161 gaaaaaaact ggaaaaagaa ggtgggtaat gtcattgaac agctcgaggc cataaacttt
    14221 accgatcttc ctgagatgga agatattagt gttataaaag aaggcattgg taaaggatca
    14281 ggctacgaat tcctcaaaaa atatgacaaa ctgatcgacc ttggacttga atcatggcag
    14341 taccactttg agttcctgaa tttatcctac gctgtccagg tgaacttctt ccagactatg
    14401 aatcaactct ttcccggcat accaatggca accctgacca agatgtctgc cggttttgat
    14461 cccattctct tcaggcctga cgcggagctt atcaggcttg ccaagctcgc ccttgagata
    14521 ggctgcgaag aactcattct gaaacctttg aaggctgatg agctgatgaa ggctttagct
    14581 gaggactcca taggtaaaat atggctccag gagctcgaga gaatcagata cccctggttc
    14641 catatctctg ttggtaccgg gtggtatcac acccacatca gttggaatga caacctggag
    14701 attcccttcg acaacatcag gaccaacata agggctatca aggcagggaa aacactcggg
    14761 agaccaactg atcagattct gaaagaacga gacagaattg caacagaata tagagccctc
    14821 cttaaaactg atgcggatag agaagccttt gaccagggtc ttaaccttgc acgacttgtt
    14881 cttccttatg cagaagacca caatttctat attgagcact ggtttcacac tctcttctgg
    14941 ggtaaggtcc gccagatagg acaggtcctc accaatgcag gtttctttgg agacgcagat
    15001 gacatctggt acctgagacg agatgagatc aaacaggcgt tgtgggatct ttgtacagcc
    15061 tgggcaacgg gtgtaaaacc aagaggcccc tcttattggc ccaaagagat tgattggaga
    15121 aagggagtat atgagaaatt caaagagtgg acaccaccag cagcgcttgg cacacctcca
    15181 gaggtcgtga ccgaaccatt tacaatcgta ctctggggcg taacaagtga cgtgctgaaa
    15241 acatggctca aaggcggcga agaagacgga ggggatccaa atgaacttca aggttcccct
    15301 ggttcgtccg gcatcgctga aggtaaggcg cgggtactca agagtgtcga ccaacttgta
    15361 gatttaaagg aaggcgaaat tctggtggca accaccacat cacccagctg ggcacctgcc
    15421 tttgtgaaga ttgcaggggc aatcacagac gttggcgggc ctatgtgcca cgcagctatt
    15481 gtttgccggg agtatgggtt gcccaccgtt gtgggaactg gaaaaggcac tcagttcata
    15541 aaaactgggg acctaatcag gatggatggg gacacaggaa aggtcaccat tctccaaaga
    15601 gcataaaatt accattcttg acgtcaaggg aatggggctt cttgacaaac cccattccca
    15661 agagacggta ctttggagga gaacatggga gataaagaca aaagatatat cttatggtat
    15721 gacgaaatgg agggagcgga ctttcccatt gtgggcaaaa agaactccaa tcttggcgaa
    15781 atgataaagg ctggaattcc cgtctcccat ggttttgcta ttaccatcta cgcgaatgat
    15841 cagttcctcg ttctgactgg tattaaagaa gaaattgaga aacagataac atcgctggga
    15901 aaggtaacct atgagactgc aaaacaggcg agcgccattg caatgaattt gatagaaaaa
    15961 gctaagatgc cggaaatcat tgaacgtgag atactcgaca actataagaa actctgtgat
    16021 caaacctgca tgagtgcttg tcctgtggct gtaaggagta gcggcgctgt atcaatgcct
    16081 gggcagatgg aaacatacct gaatatttgc ggtgagaatg accttattga ctacgtaaaa
    16141 aaatgctggg caagcgcata caatactgag gcgattatgt accggatgaa caaaggtatg
    16201 cccttcctct ttaacatcgg cgtaggcata ccaaagatgg tcaattctcg tgtttccgga
    16261 attattttca ccatcaaccc tattaacgga gatccttcaa agatatcgat tgatgcaagt
    16321 tatggacttg gcgaagccgt cgtcagcggg cttgtaactc cggacactta tttaatagac
    16381 aaagtcactt ttgatataat caaaacaacc gtgggtagta aggaaaccat gtgtgtctac
    16441 aaagatgaag gaagtgatat tgttcaaaaa gacgtcccgg aggaaaagag aggggtctcg
    16501 tcgctttctc aacaggaaat aaaagaactg gccagaatag gcaaactgat cgagcagtac
    16561 tatggaaaag cgtatgatat tgagtttggt attgatgcag actatccatt ccctgagaac
    16621 attattatat tgcaggtcag gcctgaatca gtttggagca agaaagaggt ggaggcgaaa
    16681 gtggaggcca aaaaagatcc tatggatcgt ctccttagca ctcttcttgc cggggtaaag
    16741 ataaaatgag cacagactgt gttctcattt attcaaaaaa actgtaagga ggtgatgctt
    16801 tagataagca aaacagaaat acaattattt tgtaaacttt acttgttatc cacaaacaaa
    16861 tgagctttga acaggaagga gatatcttgc agttagtagg ctttcagcag aaatcttact
    16921 aaaaacccga ataggggatg gttactatgg agcagcaagg agggttgaag cagcctgcat
    16981 taggaattgt cgggcttttt gtggtagtgt tcattgcctt cggaatcacc acctggttta
    17041 aaccggaaac gtttatccca tgggcaggag agctggctat gtgccttata ccaactgcca
    17101 tcataatggg catggtctgg cagggcaatt atccgccacc agcggtcagc ctggctcagc
    17161 ccttaaaaag tacctatctt ctcttcttaa acatgcttgt gggtgccctt gtcgcaggat
    17221 actcgatcaa gaccgtggga gtctttgtga cgcccccaac tcccccactt atattcttca
    17281 caataatgac tgtaatcatg acgttctggt gtgttgttgt gtggagatgc tggccggcgg
    17341 caggtattaa ggacaaccat cccgcctttg tcggttttgg aacacttatc gtctcatatg
    17401 cggtagccta catcctctgg aaaatatttt ttaattttga tttcatgaag ggtgctcctt
    17461 tttatgatgc tgtagcactg ccaagcggag ctttctttgc cttttggagt ctgggtttct
    17521 ttctcacgtg ccttgccgtc atcctggcat gggtcgagct tgacttctgg ccgctgagtt
    17581 caattccggc aaaagtgcct gcattcggta cacaacccct gtggggtact gtggttagta
    17641 tgactgtttt cttatttaaa caatgatact aac
//
LOCUS       DAOOMC010000057        17473 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdus sp. isolate AMR_MDS_2685
            NODE_3385_length_17473_cov_6.015214, whole genome shotgun sequence.
ACCESSION   DAOOMC010000057 DAOOMC010000000
VERSION     DAOOMC010000057.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864760
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdus sp. (wastewater metagenome)
  ORGANISM  Syntrophorhabdus sp.
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae; Syntrophorhabdus.
REFERENCE   1  (bases 1 to 17473)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17473)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,729
            Genes (coding)                    :: 2,708
            CDSs (with protein)               :: 2,708
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 7 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17473
                     /organism="Syntrophorhabdus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3385_length_17473_cov_6.015214"
                     /isolate="AMR_MDS_2685"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2699756"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(92..265)
                     /locus_tag="PK125_07250"
     CDS             complement(92..265)
                     /locus_tag="PK125_07250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB37940.1"
                     /translation="MKMKNSDLVPGLHKPPASIDILSSIIKTTAVVTLNLLEQLKKRI
                     VKESKEYRKGYYF"
     gene            677..1303
                     /locus_tag="PK125_07255"
     CDS             677..1303
                     /locus_tag="PK125_07255"
                     /inference="COORDINATES: protein motif:HMM:NF014375.2"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HPB37941.1"
                     /translation="MNETLINLMADLQEEETLAMVNTMIKEGVNPMDILSDARSAMEI
                     VGNRFETSEYFIPDLMMAGEIMKGISDIVKPLLQSDDASAKKGKVLIGTVAGDIHDIG
                     KDIVTFMLDVSGYDVLDIGIDVPVQTFVDKIREFQPSVVGLSGFLTLAFDSMKKTVEA
                     IEKAGLRDNLKIMIGGGQMDDEVRKYVRADAYGKDAVAAVNLCKQWVG"
     gene            1313..2563
                     /locus_tag="PK125_07260"
     CDS             1313..2563
                     /locus_tag="PK125_07260"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HPB37942.1"
                     /translation="MAPTPQEQYKEREKRVLDAIALKKPDRVPIMPHFSFFSAKYSGF
                     TPEEVMYDPEKLWKAQWRTLTEFEPDMDNSPFGLRFLGPLLETLDFRQLKWPGHGLSS
                     DVSYQFVEGEYMKAEEYDVFLGDLTDFMIRTYWPRIFGSLKAFEKLPPLRDIITYSIG
                     TAFGFGIFGSPEVVTALDALKKAGEKSLQISQYSQLFAEKAREAGFPMQYGGYSQAPF
                     DTLGDYFRGTKGAMLDMYRRPDKIIKACEKLLPIMFEVAVNASKVSGNPRIFMPIHKG
                     IDGFMSEEQFKKFFWPTLQSLIVALINEGLTPCVLWEGNCTTRLDIIKDIPEGKAIYA
                     FEATDIFKAKEVLGHKVCIRGNVPLSLLVTGTTEDVQVYCKRLIDVVGKDGGYIMDAA
                     AGFDDAKPENVKAMFDHTREYGVY"
     gene            2656..4416
                     /locus_tag="PK125_07265"
     CDS             2656..4416
                     /locus_tag="PK125_07265"
                     /inference="COORDINATES: protein motif:HMM:NF010032.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HPB37943.1"
                     /translation="MPYDPTRMANWKISEAAEENMKSIWQLGDELGLEHDEIIPHGKI
                     GRIAFAQIMERLKEKSDGKYVEVTAITPTPLGEGKTTTTIGLIEGLSRRGKHAGGAIR
                     QPSGGPTMNVKGSAAGGGNAQVIPLAEFSLGLTGDINNVMNAHNLAMVALTSRMQHER
                     NYGDWELGLRKLRRLSIDPSNVQMGWVVDFCGQALRNIVIGLGGRMNGFTMQSRFDIA
                     VSSELMAILSIARDLKDLRERIGRITVAYDTYGKPVTTEDLEVAGAMCAFMRESINPT
                     VVQTIEGQPILVHAGPFANIAIGQSSIIADRVGLKLFDYHVTESGFAADIGFEKFWNV
                     KCRISGLTPNVSVITATIRALKMHGGGPRVAPGIPLAKEYTKEDLTLLEKGIPNLLHH
                     IRTVKMSGMKPVVCINRFSTDTREEVALVKKYAEAEGARFALSDHWMKGGEGALELAD
                     AVIDACNEKVDFQFLYPLEMPLRDRVELIATKIYGASTVLWIPSAEAKLNTLQADPGK
                     KDYFTMMVKTHLSLSHDPDLKGLPTGWRLPVRDILVFTGAQFLCPVTGDISLMPGTGS
                     DPAFRRIDVDTETGRITGLF"
     gene            4457..5344
                     /gene="folD"
                     /locus_tag="PK125_07270"
     CDS             4457..5344
                     /gene="folD"
                     /locus_tag="PK125_07270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001843924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HPB37944.1"
                     /translation="MAAKIISGAEIASSIREEIKTDAAKLKDKHNIVPGLVTIIVGKN
                     PASVSYVTAKQKTAKELGFYSVQEDVDEAVPEAELLNLIDKYNSNPQIHGILVQLPLP
                     PHINETKILYAIDPDKDVDGFHPVNLGRLVIGEARFYPCTPYGIQQMLVRTGVVVDGA
                     EVVVVGRSNIVGKPVAMMLVQKMKGANATVTICHTATKDLAAHTRRADILIVAAGRPK
                     AITADMVKEGAVVIDVGVNRIGVTPEGKAKLCGDVDFDAVKERASAITPVPGGVGPMT
                     ITMLMLNTLKAAQWTIKTA"
     gene            5380..6234
                     /locus_tag="PK125_07275"
     CDS             5380..6234
                     /locus_tag="PK125_07275"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: protein motif:HMM:NF013007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HPB37945.1"
                     /translation="MITQLVSPKGKEVLVGGVGPTILIGEKINPFGKGPIKEALISGN
                     MEPVRDEAIKQVEAGADVLIISVAAFGIDEVVTLPKATQAVMEAADVPICIESRNPVA
                     LEKALQLGCGKPIISSVTGEAIHLDQLLPLVKKYNTAFVALASDSSGVPGTAERRLEI
                     ITDIVKKADGLGIDRANIIVDCVAESSAVNSNAATITLEAMDMVKQLWGLNLILGASN
                     VSFGLPSRTVINAVFLSLAVSRGLTLAIVNAKLMKPYIMAADLLMGKDNRARRYTAYC
                     RKLLKANA"
     gene            6227..7114
                     /locus_tag="PK125_07280"
     CDS             6227..7114
                     /locus_tag="PK125_07280"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HPB37946.1"
                     /translation="MPEYDVNYRENVVATLKHENPSYLPRGEIFFTRTFLNVIFPEFI
                     DDYIEQLASAASLLDLSVVGVDLNDEWSRSLLSQGKYSRLKKYFTVGYINGPVLRSIE
                     RLGFKQAMLGIKKDRGTVFRITSDILDDIQDVCSLAKKNEMSGILLPDDIAGNNGLYF
                     SFEDFGTAILPLYKQIASEIKGNDLFAFFHSDGDTRLIIEPLIKAGFDCIHPVDSQAG
                     LDLYELKYIHKNMISFMGHIDIMTWDDVRINQEIDKAEKSFNEGGLIIGSTCGLSMKT
                     VSKKLGALYPRLHKWGRRV"
     gene            7111..8976
                     /locus_tag="PK125_07285"
     CDS             7111..8976
                     /locus_tag="PK125_07285"
                     /inference="COORDINATES: protein motif:HMM:NF025925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASKHA domain-containing protein"
                     /protein_id="HPB37947.1"
                     /translation="MKKARVLFTPSQKEVEGEIGRTILDLARKAGVYIDSQCNGKGKC
                     GKCRVRVLEGDVSLFVQEEAQFVKGFEQEAGYRLACMTRVRGDAIVLVPGENVLTSVA
                     SRKMFSKRTKTINPAVKCYHIDIDVLGKDHSEYVNDIIKLLRTQYGLLNITIDPGILE
                     DLNKEPIKSKGTLGVFVWMDKEIVAIHPEKITAPLGVALDIGTTTVALYLCDLVKGEV
                     IASGSFTNPQVLFGPDIMSRISYSVEHPCEGVKRMQKELMNSINALILNLVSGQGYSP
                     EDIVDMTVVGNTVMHHIFLGIAPDNLGLWPFEPLVKGPVNVKSHKLGLSINPYSYVHM
                     LPVEAGFVGSDNVGVLISEEPYVKDELSLIIDVGTNGEVVLGNKDQLFSCSCATGPAL
                     EGAQISCGMRAAPGAIEKIRIDPEDFEVNYKVIGRKGWANNLPAGTLKPSGICGSGII
                     DMVAQLFKARLINTNGAFSNKTNTPRIRKGKTGVAEYVVAWKKETSTQDDIVFTQKDV
                     RQVQLAKAALHGGCRVLMSRLNIDTLRGLTIAGAFGMHIDKESALTLGLFPQCEPGNI
                     VMVGNAAGHGAYLALVDRSKRDEADSVARMVTHIELAMEEGFQGEFMKALAIPIH"
     gene            complement(9047..9469)
                     /locus_tag="PK125_07290"
     CDS             complement(9047..9469)
                     /locus_tag="PK125_07290"
                     /inference="COORDINATES: protein motif:HMM:NF024854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HPB37948.1"
                     /translation="MAVVRIAEVFKRSVSAAFRKYELTFPQYNVLRVLDASKNGQSRI
                     TNVSRIMLVPGANMTGIAKRLEKSGFLTRKSDPSDERATVLSITPKGKAMLTKIEKER
                     DRHMRTMLKGFSEDEKQELLSKIKRLIKNSGQTFQNIR"
     gene            9806..11263
                     /locus_tag="PK125_07295"
     CDS             9806..11263
                     /locus_tag="PK125_07295"
                     /inference="COORDINATES: protein motif:HMM:NF013714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HPB37949.1"
                     /translation="MSIKSDLAKIVGKDYATDSMEERKAYSTDYSLLPPGLPDVVVWP
                     ETSEQVGKIVAWCNDRNIPVVPVSSKVHFHGCTIPKQGGIVVDMSRMNKIQEIDLDNR
                     LARVEAGVTWEQFVAALEKKGMRTIMPLLPPGNRSVLTDTLERETPTNIVYDYGEPMQ
                     SIEVVWPNGEVFRCGSASVNGFPDSKSRGANPSGPGIDFYRFVQQAQGTMGIVTWSNV
                     KIESIPKIDKIFFAPVEDLPYAIDFLYRILPRRIGQECLLLNNVDLAAIVAENMPDDF
                     DMLCATLPSWTLILVISGLLRRPEEKIAYEENFLSQVIKNEFPKMALADNLPGFPGLG
                     RKLLPMLRKPWPSKVPYWKNRIRGGCQNLFFITKPFKAPMFMDMVETITARYGYAVNE
                     IGSYIQPIEHNRACQIEFSFFYDPDDEGEKSLVAEIYREAAVAMMNEGAFFTRPYGDL
                     APIIYNRAAGYTMTLKRLKAIFDPNNIMNPGNLCF"
     gene            11274..12593
                     /locus_tag="PK125_07300"
     CDS             11274..12593
                     /locus_tag="PK125_07300"
                     /inference="COORDINATES: protein motif:HMM:NF014774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HPB37950.1"
                     /translation="MRKDEMFDTKKVKVALDVTDLKNFVYDMQHCVKCKGCYWVEHTY
                     VPGIDFSVRCPSNLWNDFDSYGAFGKMRIGLAVEEGKLEWTDKLLEIIYADPLCGACD
                     VGCKRNLDLEIELSLEALRIKAVKDGAGPMPAHKKIAKNIATKHNQFGSPHENRKNWI
                     TKDVQVAEKADIVYFVGCSASYVNPEIARATAKVLNASGNKFMLMSDEWCCGNTLFSV
                     GMVDEARDLAKRNIAAVKKTGAKKLVTSCAEGYRMWKVDYPKLLDIATSDLGFDVVHL
                     IEVADDAIKSGNLKLTKPVDVRFTYHDSCGVSRLSDPWTPWKGERGWMGMVSPPLKRR
                     RGKSGLYIQSRNILNAIPGAKFVEMPRIRENAFCCGAGRGVKGAFPDLAKFSANHRLD
                     EVKSVSAEVLVSACPWCKSNFSQVAKENGNSVKVMDIAEIISAALER"
     gene            12605..14215
                     /locus_tag="PK125_07305"
     CDS             12605..14215
                     /locus_tag="PK125_07305"
                     /inference="COORDINATES: protein motif:HMM:NF013714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HPB37951.1"
                     /translation="MSIQKQAYSVLEAAVGAKYISDDPAICEGYRSGPAGYENGLGYE
                     RVMTTIPGAVILPKNTEEVQKVVKICNRYKVPYVPYSTGFYGPRSHCHVENELLIDLK
                     RLNSFEIDEKHFYVVVGSGVVYSPIQQEAMNKGAYVVIGGGGAQASAIANLIGDGWSP
                     LSHRIGLPHRRILGTELVLPDGELVKMGSLADGADPFWGDGPGPDLRGLLRGFTGLRG
                     CLGIVTRMAIKTLPFQPEPLVPTGISPNTALALPEKRVKWVNFQMPSKAAQVKAMFEI
                     AKAEIAGAVTKVPLFWRAIAKAECKEEFWEIWGKETEESIKNFFIVRVLLIGYTSEEQ
                     MQYDENVLTDIMTELGGIARPTKPSDESWIKNADSAGMWLMCGSYVSVDYIIESLSQA
                     TVHGESYAELKRKYTPPLMPDNGDPGWFQSFELGHQGYSEFLIYWDQREDTTGVDHFY
                     LETAKMNIKNRFYTSLLGPHQPLYLTGPMYGPNYHEWLLKVKDAFDPLWICHPPVPLA
                     HDEFVERAPWMKEIKDWKSPKKLPMPKW"
     gene            15042..15302
                     /locus_tag="PK125_07310"
     CDS             15042..15302
                     /locus_tag="PK125_07310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB37952.1"
                     /translation="MSKQYMTCPFSKGKCIECAIYRGRHAEICFAAGYHGGEFKDLMK
                     PKAKPTNPTTELKFPFPSISESPNRISNVEDCVLTGDGVKTK"
     gene            15330..16202
                     /locus_tag="PK125_07315"
     CDS             15330..16202
                     /locus_tag="PK125_07315"
                     /inference="COORDINATES: protein motif:HMM:NF013134.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein subunit M"
                     /protein_id="HPB37953.1"
                     /translation="MIHDFTYLKPGSVKEALAMLADHAEECKVICGGQSLLIVMRQGL
                     VAVDYLIDLKGLNELSYIKYDAKEGLKLGATTTHRMIEKSDIIKKNYPVLAIMEEKLA
                     SVQVRNWGTIGGNLAHADAAGDPAPVLISLGATVKLGSKKGDRVMALEDFYTDLFETA
                     MEQGEMILEVQVPPPPPKTGSAYQKFNLIEDDQGIVAVAVTVTADKSGVCSDAKIILG
                     NAGVTPIRAKTAEKILIGNKLTDKLMVEAGEAAAGDADPVSDIHASADFRRHLIKVLT
                     KRMVKQAWDQAIKG"
     gene            16217..16702
                     /locus_tag="PK125_07320"
     CDS             16217..16702
                     /locus_tag="PK125_07320"
                     /inference="COORDINATES: protein motif:HMM:NF013923.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HPB37954.1"
                     /translation="MEKKLITLTVNGIDYELYLNPKTLLVEAIRDHIGLTGTKRGCET
                     VSCGACTVMIDDMAVKSCSVLAVQAEGHRITTAEGLEKNGELAPIQKAFLDEGSYQCG
                     FCTSGMLMSAEAFLKETKNPSEEQIKEAIEGNICRCTGYNSIVRAIAAVAKGKYAEVK
                     S"
     gene            16699..>17473
                     /locus_tag="PK125_07325"
     CDS             16699..>17473
                     /locus_tag="PK125_07325"
                     /inference="COORDINATES: protein motif:HMM:NF013482.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HPB37955.1"
                     /translation="MNKYTVINPHEPFRVLNTHVHNIDGIAKVTGRATYTFDVKLPGM
                     LYGKILRSPYPHAKIVKIDASKALALPGVIAVANGKDDTLGIKQGIWRRYKDLCDEQV
                     LPVDKVRYIGEPVVAVAAITEEIAEKALDLIDVEYEELPATFEPLDAIKKDAPEIHEG
                     FERNINVTRHIEWGDVEEAFEEAEYIREDWFKCGGQAHMCMETRAAVASYTPDGKMTV
                     WTSTQSAYYHQALLAGVLGLREGNVRILAPYVGGGFGGKF"
BASE COUNT         5031 a         3497 c         4339 g         4606 t
ORIGIN      
        1 gaagaacaga taatgagaat atcctttagt ttccgcttgg tttcaagaac aggcactgct
       61 ttcaagataa agcccctgag tgaggcatta tttaaaaata gtaacctttt ctgtattctt
      121 tactttcttt aacaattctt tttttcagtt gttccagtaa atttaaggtt actactgcag
      181 tcgtcttgat aattgaactc aaaatatcga tggatgcggg gggcttgtgt aagccaggaa
      241 caagatcgct atttttcatt ttcattaaag ccctgccaga gtttcttcag gtttctcgtt
      301 gaatcgttgg gatgggacaa gttttttctc tttgagccat gatagactta aggattcatg
      361 ctgtcaatcg aaaattacac gaaatatcgt taatttaact tcatatcgta ataaatatgt
      421 taggtaaatc aggttttgat gagaatgtct catgataata aatagttgca tttttggcat
      481 ttaatttgta atagaaaatt agatataatg aacactgaag tattttgtaa aatatgcata
      541 agggtgtatc caaaacatgc accttagaga ttattacgga taggcaacag aagacggtat
      601 caggcgggcc agacatgaag acgtcaaacg cccctaactt tcgcaaaccg gcatctacta
      661 aaaaaggagg cacaatatga atgaaacatt gattaacttg atggcggacc ttcaggaaga
      721 agaaaccctc gctatggtca atacgatgat taaggaagga gtaaacccta tggacattct
      781 cagtgatgca cggtctgcca tggagatagt gggaaatcgc tttgaaacgt cagagtactt
      841 tatccctgat cttatgatgg ccggagagat catgaaaggc atctccgaca ttgttaagcc
      901 ccttcttcaa agcgatgatg ccagtgcgaa aaaaggaaag gtattgatag gcacggtggc
      961 aggagatatc cacgatatcg ggaaagacat cgtcacattt atgctcgatg taagcggtta
     1021 tgatgtcctt gatatcggaa ttgatgtacc ggtccagaca tttgtagata aaataagaga
     1081 gtttcagcca agtgttgtcg gcttaagcgg atttctcacc cttgcctttg attccatgaa
     1141 gaaaacagtt gaggccatag agaaggcagg tttgcgggac aacttgaaga tcatgatcgg
     1201 tgggggtcag atggatgatg aagtcagaaa atatgtacgc gccgatgcct atggaaagga
     1261 tgccgtggcc gcagtgaacc tttgtaaaca gtgggttgga taggagagta atatggcgcc
     1321 aacaccacaa gaacaatata aagaacgtga aaaacgggtt ttggatgcta tagcccttaa
     1381 gaagccggac agggtgccca taatgcccca cttcagtttt ttttctgcaa aatactccgg
     1441 ttttacccct gaagaggtta tgtatgatcc agaaaagttg tggaaagccc aatggagaac
     1501 cttgacagag tttgagccgg acatggataa tagccccttt ggccttcgct ttctgggacc
     1561 tttgctggaa actcttgatt tcaggcaact taaatggcct gggcatggcc tttcatccga
     1621 tgttagctat cagttcgtgg aaggcgaata tatgaaggcc gaggaatatg atgtttttct
     1681 tggtgatctt actgatttta tgattcggac atattggccc cgcatatttg gatcactgaa
     1741 ggcttttgaa aagcttccgc ctctcagaga tatcattacc tattctatcg gcactgcctt
     1801 tggttttggt atttttggtt caccggaggt cgttacagcc ctggatgctc ttaaaaaggc
     1861 aggtgagaaa tcccttcaaa tcagtcagta ttcccagtta tttgctgaga aagcgaggga
     1921 ggctggattt cctatgcaat acggtggata ctctcaggcg ccctttgaca cccttgggga
     1981 ttattttaga ggtacgaaag gcgccatgct cgatatgtac agaaggccgg acaaaattat
     2041 aaaagcgtgt gagaagttac ttcccatcat gttcgaagtg gctgtaaatg catcaaaagt
     2101 gtcagggaat ccaaggatat ttatgccaat tcataaaggg attgatggtt tcatgtcaga
     2161 agaacagttc aagaagtttt tctggccaac cctacaaagc ctcattgtcg ccctcattaa
     2221 tgaaggatta accccttgtg ttttatggga aggcaattgt actacccgcc tcgacatcat
     2281 caaggatata ccggaaggca aagccatata tgcttttgaa gcgacagaca tcttcaaagc
     2341 caaggaagtt cttggacata aagtctgtat ccgtggcaat gtgccgttgt ccttgcttgt
     2401 aacgggtacg acagaagatg tacaggtgta ttgtaaaaga ttgattgatg ttgtgggaaa
     2461 agacggtggt tatattatgg acgctgctgc tggctttgac gatgctaagc cggaaaacgt
     2521 gaaggctatg tttgatcaca ccagggaata cggtgtctat tgaagggttg ttgcttatag
     2581 ttgtttgcgc tgcttccggg tattttttac acataatggt atcacgacat agaaatctgg
     2641 tataaggagg attgcatgcc ttacgatcca acacgaatgg ctaactggaa aatttccgaa
     2701 gccgctgaag aaaacatgaa atctatctgg cagctcggag atgagctggg tctcgaacat
     2761 gatgaaatca ttccccatgg caaaattggg cgtatcgctt ttgcccaaat tatggaaagg
     2821 ctcaaagaga aatcggatgg aaaatatgta gaagttaccg ctattacacc aacccctctc
     2881 ggtgaaggca aaacaaccac tactatcggt ttaatcgaag ggctgagcag gagaggaaaa
     2941 catgcaggcg gagctattcg tcagccatcg ggcgggccaa cgatgaacgt caagggaagc
     3001 gctgcagggg gcgggaatgc ccaggtgatt cctttggctg aattctctct aggccttact
     3061 ggtgatatta acaatgtaat gaatgcccat aatctcgcca tggttgcatt aacatcaaga
     3121 atgcagcatg aaagaaacta cggagactgg gaactggggc tgaggaagct tcgacgcctc
     3181 agtattgatc ccagtaatgt gcagatggga tgggttgtag atttttgtgg ccaggccctg
     3241 aggaatattg ttattggcct tggcggaagg atgaacggtt ttaccatgca atcacggttt
     3301 gatattgcag taagctcgga acttatggct atcctttcaa tcgccaggga tcttaaagat
     3361 ctacgcgaac gaattggacg gataaccgtg gcatatgata cctatggaaa gcctgtaaca
     3421 acagaggacc ttgaggttgc aggtgccatg tgtgccttta tgcgggaaag cataaacccg
     3481 accgtggttc aaaccattga aggccagcca atactggttc acgcagggcc ttttgcgaac
     3541 attgccattg gccagtcgtc cattatagca gatcgtgttg gtctgaaact ctttgattac
     3601 catgtcacag aaagtggctt tgccgctgat attggttttg agaagttctg gaatgttaaa
     3661 tgcagaatca gcgggttgac ccccaatgtg tctgtcatta cagcgactat aagggcatta
     3721 aagatgcatg gagggggccc aagagtggca cctggcattc ctcttgccaa agaatacact
     3781 aaagaagatc tgacgctatt ggaaaaagga attccaaacc tccttcacca tatccgaacg
     3841 gtaaagatgt cagggatgaa gcctgttgtc tgcattaatc gtttttctac agataccaga
     3901 gaagaagttg cccttgtaaa aaaatatgct gaagcagagg gcgcacggtt tgctctatca
     3961 gaccactgga tgaaaggcgg tgaaggtgcc cttgaacttg ctgatgcagt catagatgca
     4021 tgtaacgaaa aggttgattt tcagtttctt taccctcttg aaatgccatt aagggatagg
     4081 gttgaactga ttgcaacaaa gatatatggt gccagtaccg ttctttggat cccttcagca
     4141 gaggcgaaat tgaacacatt gcaggctgac cctgggaaaa aagactattt taccatgatg
     4201 gtcaagacgc acttgagtct ttctcatgac cctgatctga aagggcttcc aacaggctgg
     4261 aggcttcctg tcagagatat cctggttttc acaggggccc agttcctgtg tcctgttacc
     4321 ggtgacatca gccttatgcc aggcactgga tcagacccgg ctttccggag aattgatgtt
     4381 gatacagaga caggaagaat tacagggtta ttctaatgga gacagagcat tgttataaga
     4441 aaaaaagagg tgttgtatgg cagctaagat cataagcggt gctgaaattg catcctcaat
     4501 ccgtgaagag attaaaacag atgcagcaaa acttaaggac aaacacaaca ttgttccagg
     4561 gctggtaaca attatagtag gcaagaaccc ggcatcagtg agctatgtga ccgccaaaca
     4621 gaagacggca aaggaacttg gtttttattc agtacaggaa gatgttgatg aggcagtacc
     4681 agaagctgaa ctcttgaacc ttatcgataa atataacagt aatccccaaa ttcacggtat
     4741 tctcgtccag ttgcctctgc ctcctcacat caatgaaacg aaaattctct atgccattga
     4801 tcctgacaag gatgtagatg gttttcaccc ggtcaatctt ggcaggcttg taataggcga
     4861 ggcaagattc tatccgtgca ctccttacgg aattcaacaa atgctcgtga gaaccggtgt
     4921 tgttgttgat ggggcagaag tggtcgttgt aggtagaagc aatatagtcg gaaagccagt
     4981 tgcaatgatg ctcgtccaga agatgaaagg tgcgaatgct acagttacca tatgccacac
     5041 agcaacaaaa gatcttgctg cccatacacg aagggctgat attcttattg ttgccgcagg
     5101 taggccaaag gctataaccg ctgatatggt aaaggaaggg gctgtggtca tcgatgtcgg
     5161 ggttaatagg ataggtgtga cgcctgaagg aaaggcaaag ctctgtgggg atgttgactt
     5221 tgatgcagta aaggaaaggg catccgctat tacccctgtt cccggagggg tagggccaat
     5281 gaccattaca atgctcatgc tcaatacctt aaaggctgca cagtggacca taaagactgc
     5341 ataacattgg atgtgatgta ctttaaatag gaggcagtga tgataacgca attagtgagc
     5401 ccaaaaggga aggaagtcct tgttggtggt gtggggccca ccatactcat aggtgaaaag
     5461 atcaaccctt ttggaaaggg accaatcaag gaggcactca tatcgggaaa tatggaacct
     5521 gttcgtgatg aggctatcaa acaggtagaa gcaggtgccg atgtgttgat tataagtgtt
     5581 gctgcatttg gcatagatga agtagttact cttcccaagg ccacacaggc tgttatggaa
     5641 gcagcggatg ttcctatttg tattgagagc aggaatcctg tagcgcttga gaaggctctt
     5701 caactcggat gtggaaaacc aataataagt tctgtcacgg gcgaagcgat acaccttgat
     5761 caacttcttc cattggtaaa gaaatacaat accgcttttg ttgccttagc ttcagactca
     5821 tcaggggtac caggtactgc tgaaagaagg cttgagatca ttacggatat cgtgaaaaag
     5881 gcagatggac ttggaataga tcgtgcaaat ataattgttg attgtgttgc ggagtcgagt
     5941 gctgttaata gcaatgcggc aacaataacc ttagaggcta tggatatggt gaaacagctc
     6001 tggggtctca atcttatctt gggggcaagt aatgtctcat tcggcttacc gagcaggaca
     6061 gtgatcaacg cagtctttct ttctcttgct gtttcccggg ggcttaccct ggccattgtg
     6121 aatgcgaagc tcatgaaacc ttacatcatg gcagcagacc ttctgatggg aaaggataac
     6181 agggcaagaa gatatacagc ctattgcagg aaattgctga aggcaaatgc ctgagtacga
     6241 tgtgaattac agagaaaacg tagttgcaac ccttaagcat gaaaacccat cttatttgcc
     6301 caggggcgag atctttttta ccaggacatt tctcaacgta atctttcctg aattcattga
     6361 tgactatatt gagcaactgg cttctgcggc atcccttttg gatctttccg tggttggggt
     6421 tgacctcaac gatgagtggt ccagatcgct attaagccag ggaaaatata gcaggctaaa
     6481 gaaatatttt actgtagggt atatcaacgg tccagttctg cgttcgattg agcggcttgg
     6541 gtttaaacag gcaatgttag gtattaagaa agaccggggg accgttttca ggatcacttc
     6601 agatatattg gatgatattc aggatgtttg cagtttggca aagaaaaatg agatgagtgg
     6661 tattttgctc cctgacgata ttgccggcaa taacggactt tatttttcat ttgaagattt
     6721 tggtactgcc attcttcccc tgtacaaaca gattgcaagt gaaatcaaag gaaacgatct
     6781 ttttgcgttt tttcactctg acggggacac acgcttgatc attgaacccc ttataaaagc
     6841 gggatttgat tgtatccacc ctgttgatag ccaagcaggg cttgaccttt acgagttgaa
     6901 gtatattcac aaaaacatga tatcttttat gggacacatt gacataatga catgggacga
     6961 tgtgcgaatt aatcaagaaa tcgataaagc agaaaagagt tttaatgagg gtggacttat
     7021 tatcggatca acatgtggcc tttcgatgaa aaccgtgagt aaaaagctgg gtgcgctcta
     7081 tccccggtta cacaaatggg gtaggagagt atgaaaaaag cccgtgttct gtttacacca
     7141 tcccagaaag aagttgaagg agaaatagga aggacaattc tcgacctcgc ccggaaggca
     7201 ggagtatata ttgactccca atgtaatgga aagggtaaat gtggtaaatg cagagttcgg
     7261 gtcctggaag gcgatgtcag cctttttgtc caggaagagg ctcaatttgt aaaaggtttt
     7321 gaacaagaag caggatacag gcttgcttgt atgacacgag tcaggggcga tgctattgtt
     7381 ctagttccag gcgagaatgt ccttacatca gtggcttcaa gaaagatgtt cagcaaaaga
     7441 acgaaaacga tcaaccctgc agtaaaatgc tatcatattg acattgacgt tctcggcaag
     7501 gatcattccg agtatgtaaa tgatattatt aagctgctgc gcacgcaata cggtttatta
     7561 aatatcacca ttgatcctgg tatccttgaa gatttgaaca aagagccaat caaaagcaaa
     7621 ggtacattag gcgttttcgt gtggatggac aaagaaatcg tggcaatcca cccagaaaag
     7681 attactgcac ctttgggtgt tgcgctcgat atcggtacca caacggtggc gctctatcta
     7741 tgtgatcttg taaaaggtga ggtcattgca agtgggtcat tcacaaatcc ccaggtcctg
     7801 tttggtccgg acattatgtc tcgcatttca tactctgtag aacacccctg tgaaggcgtg
     7861 aagcgaatgc aaaaagaact catgaactct atcaatgctc taatccttaa ccttgtttcc
     7921 ggacaaggtt attcgcctga ggacatcgtg gatatgacgg ttgttggtaa cactgtcatg
     7981 caccatatct ttctcggcat agcccccgac aatctgggac tctggccgtt tgagcctctg
     8041 gtgaaagggc cagtcaatgt aaaatcccat aaacttggtc tatctataaa cccttactcc
     8101 tatgtccata tgcttcccgt ggaagcaggg tttgtcgggt ctgataatgt aggggtgctc
     8161 atctctgagg aaccatatgt taaggatgaa ctttcgctca tcatcgacgt tggtacaaat
     8221 ggcgaagtcg ttcttggaaa caaagatcaa cttttttcat gttcctgtgc caccggacct
     8281 gcccttgagg gtgcacaaat ctcttgcggg atgcgagctg ctccaggagc gatagaaaag
     8341 atacgcattg atcctgaaga ttttgaggtc aactataaag taatcgggag gaaagggtgg
     8401 gcaaacaatc ttcctgcagg gactctcaag ccctctggca tttgtggttc aggcataata
     8461 gatatggtag cacaactttt taaagcacga ttgatcaata ccaacggtgc attttctaat
     8521 aaaacaaaca cacccaggat tcgaaaaggg aaaactggtg tggctgaata tgtggttgcc
     8581 tggaaaaagg agacatcgac gcaggatgac attgtcttca cacaaaagga tgtgcgccag
     8641 gtccagctcg caaaggcagc cctccatgga ggctgcagag tgcttatgag tcgtctcaac
     8701 atcgacactc ttcgggggct aactatagca ggcgctttcg gtatgcatat tgataaagag
     8761 agtgctctta cactaggcct tttcccgcag tgtgagcccg gtaatattgt tatggttggt
     8821 aatgcagcgg gccacggtgc atatcttgcc ctcgttgacc ggtccaagcg tgatgaagcg
     8881 gacagtgtag cgcgaatggt cacgcatatc gaactggcaa tggaagaagg ctttcagggt
     8941 gagttcatga aagcgctggc aattcccatt cattaaaatt gaaaagccag ggttaatgat
     9001 gaacaagcta tctttttgga tggctcacca aaaacaagtc tgtctattac cggatgtttt
     9061 gaaaagtttg accgctattt ttgatgagac gttttatctt gctaagaagt tcttgttttt
     9121 catcttcaga aaagcccttc agcattgtcc tcatgtgtcg atcccgttct ttttcgatct
     9181 tcgtgagcat ggcctttccc ttaggagtga tggatagcac tgttgctctt tcatcgcttg
     9241 ggtcggattt tctagtaagg aaaccgcttt tttcaagtct ttttgctatt ccggtcatgt
     9301 ttgcgccggg gaccagcatg atacggctta cattggttat cctgctctgc ccgttttttg
     9361 aagcgtcgag cactcggaga acattatact gcggaaaggt gagctcgtat ttcctgaaag
     9421 cggctgaaac agaccttttg aaaacctcag caatccttac aactgccatc agaacttttt
     9481 catcggcaca tagatcgctt tgatattctg cccctgtaat acttgcacct caataattta
     9541 ttagaagatt cccaatctca gggaaaagta taccagcttt aaatgtaaaa atccaaaaaa
     9601 tcaataaatt gatatttaat atattgactt ttattttttc ccatgatatt ttttgaacga
     9661 atgcatacaa catatgtaac aaaacatttt cctacatcaa gtaagtggtt gaaatagctc
     9721 acttattaag gtgcatttgc attgcatggt ctggatatta gtaaattggc agcattgaat
     9781 aaaccgtttt gtaaaaggag gtacaatgag tatcaaaagt gatctcgcaa agattgttgg
     9841 caaagattat gctactgata gcatggagga gcgaaaagcc tactcgactg attacagtct
     9901 cctcccaccg ggattgcccg atgtggttgt ttggcctgaa acctcggaac aggtcggcaa
     9961 aatagtcgct tggtgtaatg acaggaacat ccctgttgtt cctgtcagtt caaaggtgca
    10021 cttccacgga tgcactatac ccaaacaagg aggtatcgtg gtggacatgt cgaggatgaa
    10081 taagatccag gagattgatc tcgacaaccg cctggccaga gtggaggcag gtgtgacatg
    10141 ggaacagttt gtcgccgccc tggaaaagaa gggtatgcga acgatcatgc ctttattacc
    10201 tccaggtaac cggtctgtac tgactgatac tctggagcgt gagaccccga caaatattgt
    10261 ctacgattat ggtgagccga tgcaatcaat agaggttgtc tggccaaatg gtgaagtttt
    10321 tcgctgtggt tcagcaagcg taaatggttt tcctgactct aaatcaagag gtgcaaaccc
    10381 atcggggcca ggtatcgact tctaccggtt tgtccagcag gcacaaggca ccatgggtat
    10441 cgtgacatgg tcgaacgtaa agatcgagtc tattccaaaa atagataaga ttttttttgc
    10501 tccagttgag gatcttccat atgccataga cttcctctac aggattttac cgagaagaat
    10561 cggacaggaa tgtctgcttc tcaacaacgt cgatcttgct gctatcgtgg cagaaaatat
    10621 gcctgatgac tttgacatgc tttgtgctac actgccctcc tggacactta tcctggtgat
    10681 cagcgggctc ctaagaaggc ccgaggaaaa aatagcttac gaagagaact ttctttccca
    10741 ggtcataaag aatgagtttc caaagatggc tcttgctgac aacctgccag gttttccggg
    10801 ccttggaaga aaactccttc caatgctgag aaaaccatgg ccatccaagg taccctattg
    10861 gaaaaaccgt attaggggtg gttgccaaaa tctcttcttc ataacaaaac cctttaaagc
    10921 tccgatgttt atggatatgg tggaaacgat cacagctaga tatggctatg cggtaaatga
    10981 aataggtagt tacatccagc ctatagaaca taaccgggcc tgccagattg aattcagttt
    11041 cttctatgac cctgatgatg aaggggaaaa atctttagtg gcagagattt atcgtgaagc
    11101 tgccgtggca atgatgaatg agggtgcatt tttcacaaga ccctatggag acctagcgcc
    11161 cattatatac aatcgtgctg ccggttatac tatgaccctg aaacgattga aagcaatatt
    11221 cgatcccaat aacattatga accccggcaa tctctgcttc taaggaggat aaaatgcgaa
    11281 aagatgagat gtttgatacg aaaaaagtga aggtagcttt ggatgtaact gatctgaaaa
    11341 atttcgttta cgatatgcaa cattgcgtca aatgtaaagg atgctactgg gttgagcaca
    11401 cctatgtgcc aggaattgat ttttcggtga gatgccccag caatctatgg aatgattttg
    11461 attcttatgg tgcttttggc aagatgcgta ttggccttgc agttgaggaa ggaaagctgg
    11521 agtggactga caaactgctc gaaatcattt atgctgaccc actgtgcgga gcttgtgacg
    11581 ttggctgcaa gcgtaatctt gatctggaaa tagaattgtc ccttgaagcg ctacgaatca
    11641 aggcggtcaa agacggtgca ggtcctatgc ctgcgcacaa aaagatcgca aaaaacattg
    11701 cgacaaaaca taaccagttt ggttcacccc atgagaatag gaagaattgg attacaaaag
    11761 atgtgcaggt ggctgagaaa gcggatatag tctactttgt aggatgttca gcctcctatg
    11821 tgaaccctga gattgcccgg gcaacagcaa aagtattgaa tgcctcagga aacaaattca
    11881 tgcttatgtc cgatgagtgg tgttgtggaa atacactctt ttccgtgggt atggttgatg
    11941 aagctaggga tcttgcaaaa cggaatattg ctgcggtaaa gaagacgggc gcaaagaaac
    12001 ttgtgacaag ttgcgcagaa ggataccgca tgtggaaagt ggactaccca aaattacttg
    12061 acatagcaac ctcggatctt ggttttgatg ttgtacacct tattgaggtg gctgatgacg
    12121 cgattaaaag tggtaacctt aagctgacca aacctgtgga tgtccgattc acttaccatg
    12181 attcgtgcgg cgtgagcaga ctctcagacc catggacgcc gtggaagggt gaacgagggt
    12241 ggatgggtat ggtaagcccg ccactgaaga gaaggcgcgg taaatcagga ttatacatac
    12301 agtcacgaaa tatacttaac gcaatacctg gtgcaaagtt tgttgaaatg cccagaataa
    12361 gggaaaatgc cttctgttgt ggagctggga ggggtgtgaa aggggcattt cctgatcttg
    12421 caaagttttc agcaaaccat cgcctcgatg aggtgaagtc agtaagcgct gaagtacttg
    12481 tttccgcatg cccatggtgt aagagcaact tcagtcaggt tgccaaagag aatggaaaca
    12541 gtgtgaaagt aatggatata gcagagatta taagcgctgc attggaaaga taaggaggca
    12601 gactatgagt atacaaaaac aagcatatag tgtgttagag gcagcggtcg gggcaaagta
    12661 catctctgac gatccggcca tctgcgaggg ataccggtcg ggtccagccg gttacgagaa
    12721 tggtctcggt tatgaacggg ttatgaccac tatccctggc gctgttattt taccgaaaaa
    12781 cacagaggag gtacagaaag tagtaaagat atgtaaccgc tataaagtgc catatgtgcc
    12841 atacagtact ggtttctatg ggccgcgctc ccactgccat gttgaaaacg aactccttat
    12901 tgatttaaaa cgcttgaatt cgtttgagat tgatgaaaaa catttctatg tcgtagtggg
    12961 ctcgggtgtt gtctactccc cgattcagca ggaagccatg aataagggtg catatgtcgt
    13021 aattggcgga ggcggagccc aggcttctgc tatagccaat ttgatcggtg acggttggtc
    13081 tccgttaagc cacagaatag gtcttccaca caggcgcata ctgggtacgg aactggtgtt
    13141 gcctgatggt gagctggtta aaatgggttc gctcgcagat ggagctgacc ctttctgggg
    13201 agatggccct gggcctgatc taagaggcct tctcagaggc ttcacaggtc taaggggctg
    13261 cctcggtatt gttacaagaa tggctatcaa gacacttcct tttcaaccgg aaccccttgt
    13321 cccgactggg atttcaccaa atactgccct tgctttgccc gagaaaaggg taaagtgggt
    13381 taattttcaa atgcccagca aagcagcaca ggtaaaggct atgtttgaaa tagctaaggc
    13441 agaaatagca ggtgcagtga caaaggttcc actcttctgg cgagccatag caaaggccga
    13501 gtgcaaggag gaattctggg aaatatgggg taaggagacg gaagaatcaa taaaaaactt
    13561 ttttatcgtc cgtgtcctcc ttattgggta cacttcggaa gagcagatgc agtacgatga
    13621 gaatgtttta acagatatca tgactgagct gggcggcatt gctcggccta ccaaaccatc
    13681 ggatgaatca tggatcaaga atgcagactc tgccggcatg tggctcatgt gtggctccta
    13741 tgtatctgtc gactacatca tagaatcact ttctcaggct acagttcacg gagaatcata
    13801 tgcagagctc aagaggaaat acacaccgcc tctcatgcca gataatggtg accctggctg
    13861 gttccagagc tttgaactcg ggcaccaggg ttattccgag ttcctgatct actgggatca
    13921 acgggaagat acaacaggag tggaccactt ctatcttgag acagccaaga tgaatataaa
    13981 aaatcgcttc tacacatccc ttttggggcc tcatcaaccg ctttatctga caggacctat
    14041 gtatggtccg aactatcacg aatggcttct gaaggtcaaa gacgcattcg atcctctttg
    14101 gatttgtcat cccccggtgc ctctggcaca tgatgagttt gtggagcgag cgccttggat
    14161 gaaagagata aaagattgga aaagtccgaa aaagctcccc atgccaaagt ggtagcacga
    14221 aaggtctgat tagcgccttt cccgtattct gggaaaggcg cttcagcccc cccctgcaaa
    14281 gttgcgtgag ttttcccgtc aaactcattg tgcttgttgt acagcctccc gcgaaatagg
    14341 caggccacgc ggaggactgc gcttttcagc atgagtagga tgcacatatt catcttgcag
    14401 cgtgtatgct ctttgccaag gacttgcctg aggaatctgt tgggaattct gataggatga
    14461 tactgagaaa tttaggagaa aatattgggc aagatgacgc tccgtacata taatatttcc
    14521 caacctgtat gaacctccaa taaattgctg taaaattttc aaaatgaatt aaaaaataaa
    14581 ttattaatca cttgtatgtt agataataca aaaatgcgaa gccactacca aaggtttatg
    14641 atacatgttt tggtgaaaga cgtgttcctt tggctgaacc gcagttccgg acttgaaccc
    14701 tgaactgagc aaataaaaaa tatattgttc tgaataattt caagggagtt atgaaagcac
    14761 aacatctgat attctggtat agaggactac tgtaaaacag tagcataaag ccccatggag
    14821 cttagtttaa aagcgattat tgttcatcat gatgaacatt aatttgaaga gcttttttga
    14881 aaaatatctt gaaataatag tacaatttat tataagatgt acgagcggaa ttcgtaagag
    14941 tgaacacata taagataatt ctcatgaaag tctaatggct tccataagct ttacgctcag
    15001 tataggtatg gaatgaaaga gagttatgag gaggtgagtc aatgtcaaag caatatatga
    15061 cctgtccctt ttctaaagga aagtgcatag aatgtgcgat ttacagaggt aggcatgcag
    15121 agatttgttt tgctgcaggt tatcatggcg gggaatttaa agaccttatg aaaccgaagg
    15181 ctaagccaac caaccccact acggagttaa agttcccatt cccgagtatc tctgaatccc
    15241 cgaatcgtat ttcaaatgtt gaggactgtg ttttgacagg tgatggtgtt aaaacaaaat
    15301 gatggcacta ttagtataag gagggaagca tgattcacga ttttacgtat cttaaacctg
    15361 gctcggttaa agaggcactt gccatgctcg ctgaccatgc agaggaatgt aaggttatct
    15421 gtggtgggca atcactgctc atagtcatga ggcagggcct tgtagcggtt gattacctta
    15481 tcgacctcaa gggactgaat gagctaagct acatcaagta tgatgcaaaa gaaggtctca
    15541 aactcggagc taccacaacc cacagaatga ttgaaaaatc agatattatc aaaaagaact
    15601 accccgttct tgcgatcatg gaggagaaac ttgcttctgt tcaggttcgt aactggggta
    15661 caattggtgg taacctggct catgctgatg cagcaggtga tccggcccct gtgctcatct
    15721 cgctcggcgc cacagtaaaa cttggctcaa agaagggcga cagagttatg gctctcgagg
    15781 acttctacac tgatcttttt gagacagcca tggagcaggg tgagatgatt ctcgaggtgc
    15841 aggtaccacc gcccccgccg aaaacaggct ctgcatacca gaagtttaac cttatcgagg
    15901 atgatcaggg tatcgtcgca gttgcggtga cagtgactgc agataagagc ggtgtgtgct
    15961 ctgatgcaaa aataatatta ggtaatgcag gtgttacacc gattcgggca aaaactgctg
    16021 aaaagattct tatcggtaat aaattaaccg acaagctgat ggtcgaggca ggagaggcag
    16081 cagccggtga tgcagatcct gtatcagaca ttcatgcttc tgcagatttc cggcgccatt
    16141 tgatcaaagt gctgaccaag cgcatggtca aacaggcctg ggaccaggct attaagggtt
    16201 aaggagggct tctatcatgg aaaagaaatt aattacactt acagtaaatg gcatagatta
    16261 cgagctgtat ttaaacccca aaactctcct tgtggaagcc ataagggatc acatcggcct
    16321 tacaggaaca aaaagaggtt gcgagaccgt ttcctgcggt gcatgcacgg ttatgattga
    16381 tgatatggct gtaaaatcat gctcagttct tgcagttcag gcagaaggac accgaataac
    16441 cacagcagag ggtctggaga agaacggaga gttggctccg atccagaagg cattccttga
    16501 tgaaggctcg taccagtgcg gtttctgtac ctcaggcatg cttatgtctg ctgaggcttt
    16561 tctgaaggaa actaaaaacc ccagcgaaga gcaaatcaaa gaggctattg aagggaatat
    16621 ttgccgttgc accggttata atagtatcgt acgagccatt gcggctgtag caaaaggtaa
    16681 gtatgcggag gttaaatcat gaacaaatat actgtaatca atccacatga accattcagg
    16741 gttctcaata cccatgttca caacattgac ggcattgcca aggtcacggg ccgggcgacc
    16801 tataccttcg acgtgaagct tcctggaatg ctctatggaa agatcctccg gagcccatac
    16861 ccacatgcaa aaattgtcaa aatagacgca agtaaagctc tggcacttcc gggtgttata
    16921 gcagttgcaa atggtaagga tgacacactt ggtataaagc agggaatctg gcgaagatat
    16981 aaggatcttt gcgatgagca ggtgcttcct gttgataaag tccgttacat aggcgagcct
    17041 gttgtggcag tggcagcgat aacagaagag attgcagaga aagctctcga ccttatcgac
    17101 gttgaatatg aagaactgcc tgctactttc gaacctcttg atgcgatcaa gaaggatgcc
    17161 ccggagatcc acgaaggctt tgagagaaac atcaatgtta cccgccatat cgagtggggt
    17221 gatgtggagg aagcattcga agaggctgaa tatatcaggg aagactggtt taaatgcggc
    17281 ggtcaggcac atatgtgcat ggagacaaga gcagcagtag caagttatac acctgacgga
    17341 aaaatgaccg tttggacatc aacccaatct gcctattatc atcaggctct tctcgcgggt
    17401 gttcttggac tcagagaagg taatgtaagg attctcgcgc cctatgtagg aggcggcttt
    17461 ggtgggaaat ttg
//
LOCUS       DAOOMC010000058        17424 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdus sp. isolate AMR_MDS_2685
            NODE_3399_length_17424_cov_6.430710, whole genome shotgun sequence.
ACCESSION   DAOOMC010000058 DAOOMC010000000
VERSION     DAOOMC010000058.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864760
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdus sp. (wastewater metagenome)
  ORGANISM  Syntrophorhabdus sp.
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae; Syntrophorhabdus.
REFERENCE   1  (bases 1 to 17424)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17424)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,729
            Genes (coding)                    :: 2,708
            CDSs (with protein)               :: 2,708
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 7 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17424
                     /organism="Syntrophorhabdus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3399_length_17424_cov_6.430710"
                     /isolate="AMR_MDS_2685"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2699756"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1728
                     /gene="pheT"
                     /locus_tag="PK125_07330"
     CDS             <1..1728
                     /gene="pheT"
                     /locus_tag="PK125_07330"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HPB37956.1"
                     /translation="VLRMIAGVSMVPAPFWMRQRIQKCGMRPINAVVDVTNYVMLELG
                     QPLHAFDYHSLREKRIEVRVANENIVFKTLDGEDRNILKGDILICDGSGPVAIAGIMG
                     GENSEITEKTRDIALESAFFNPLSIRQTARRIGIRSEASLRFEKGIDLDNVDFAAERA
                     IYLMNLTAGGVIVNGKQEVYDKKSSKSIFVNFGKIIDILGTPIEHNAIISSLRSIDLH
                     IVKEEETGFVVSVPNFRHDLDEYMDIIEEVARIYGYDRIPATTPVGSFQVPKNEKRSA
                     YFSSAKEYFTACGFHELINFAFFSVKDIENFLINSSDERFSGIPIMNPISKDYEIMRT
                     FLAPNVLKTIAYNLNRGARNLQFFEMGKVFVAGHDTMPLEYPSICFVMTGKERDYFWR
                     DSCAEFDFFDTKGVLEGFGARFRVQFDFVRSSEPFLMKNRSADVLLEGIKIGWVGEIE
                     DSVLHTYNIEQKVYSAELRFDIMMEKSKINVQYKPIPRYPQVTRDFAFLVEGAVPVAT
                     MLEQIKTVSPLITAVGVFDMFKKETKSIALRVIFQSFEETLTDDAVNALQEIIIQKVT
                     SIPGVTLRT"
     gene            1738..2025
                     /locus_tag="PK125_07335"
     CDS             1738..2025
                     /locus_tag="PK125_07335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001229265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HPB37957.1"
                     /translation="MTKLEIVNVLYEKLGFSKRECAHIVDTFFEVIKSTLSKGENVKL
                     SGFGNFVVKEKKSRRGRNPQTGKEIEISQRRVVNFRLSQVLKDAINNKNRK"
     gene            2022..2333
                     /locus_tag="PK125_07340"
     CDS             2022..2333
                     /locus_tag="PK125_07340"
                     /inference="COORDINATES: protein motif:HMM:NF024803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HPB37958.1"
                     /translation="MTSNIPDRVFYRIKEVCNLTGLKPHVLRYWEQEFKDIKPQKSSK
                     GQRLYKRKDLETIFTIKKLLYEKRFTIDGAKKYMSSQKHLVDQIKEDLLEVVKILQKG
                     G"
     gene            2333..2950
                     /locus_tag="PK125_07345"
     CDS             2333..2950
                     /locus_tag="PK125_07345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB37959.1"
                     /translation="MVKKYIILFMVVFFLSACAHGPVYTAKNESDAIKKNYHNSRLQA
                     IYNQNQGLLSELYTRFTASRIDIYKEGLGFTDFTNQKDEKMYYLMVKVRPQEITYKEK
                     TSNSDQRFSEALQTYAPKYLKYLKNSDLNRQSIDGITFGVYWAVRDVCDTYGGFIEYV
                     EFYIPKQFINEFLDGKITFQEMLLDTEVLTSLDKRHPTNVKPVFK"
     gene            complement(3046..3732)
                     /locus_tag="PK125_07350"
     CDS             complement(3046..3732)
                     /locus_tag="PK125_07350"
                     /inference="COORDINATES: protein motif:HMM:NF014244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HPB37960.1"
                     /translation="MSRKYGVPATLPYFIPLPFPPFGTLGAIIKMKGIIINKKALFDI
                     GIAGPLSGFIVSIPFIILGIKMSTIQFVTSEMAYLRLGDPLLFKILQKILIGSIPEGY
                     DLSLHPLAYAGWVGLFVTALNLLPIGQLDGGHIVYAVFGNKSKWIFAFSICLLAILTI
                     FYNPGWLLLVILLLIFGMRHPEPFDMETELDSKRKLFALIILLVFLLSFTVSPFPGLT
                     ITNENTGIPV"
     gene            complement(3849..4811)
                     /locus_tag="PK125_07355"
     CDS             complement(3849..4811)
                     /locus_tag="PK125_07355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB37961.1"
                     /translation="MKEIFIAVGTKENLASDIHILGNKWSQNPRVLVVSGKDIKATII
                     EKTSALFLEENLVLTLVDPDKAIFDELRHQLDILRERIHIVIYTTSSPPDYLKKISHE
                     MLIMDKDRETRIKKRVLAFLKQHGKKMTDKAFRALVEKIKDESNLESELMKAINYIGE
                     KSSIDSKDIHAIVTEMQEDSLISLFDTFAKLDKKGVLKVFENLIQNGVHILAIHSYLV
                     RQTRLLLQAKDMEELCRTNQDYGTFVKTLSKWKDSLEIKTSEKKHYFPYQKPFYAHKL
                     SKTGRKIPRQTLISFFNNLTMSDVKMKRGTRFDQIHVERDLLGV"
     gene            5168..6697
                     /gene="gspE"
                     /locus_tag="PK125_07360"
     CDS             5168..6697
                     /gene="gspE"
                     /locus_tag="PK125_07360"
                     /inference="COORDINATES: protein motif:HMM:TIGR02533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system ATPase GspE"
                     /protein_id="HPB37962.1"
                     /translation="MKSDETLYINPDDFPTVPLVLDGVSSRFIRENKIIPIELKNNIL
                     KVILANPDDREIIEALRATLSADVIVYTGDSQTIDEYIARFYGQGPQNINRIIEDMDQ
                     QGFEYVREEEEEDVGHLKDLASEAPIIKLVNLLISRAVDSRASDIHIEPFEDELKVRY
                     RIDGVLHDVESAPKKLQAAVVSRIKIMAKLNIAERRLPQDGRIRLKFGEREIDLRVST
                     IPILHGEGVVMRILDKESIVIDLDTLGFFPDTLASFNKIITKPNGIFLVTGPTGSGKT
                     TTLYGALDKINSPDKKIITVEDPIEYQLKGVNQIQVKTQIGLNFATTLRHIVRQDPDI
                     IMIGEIRDLETAEIAIQSALTGHLVFSTLHTNDAPSAVTRLIDMGVENFLLSSTVRGI
                     LAQRLLRNICPGCKEVDKTRANQEEFSALGMTGDIPLYKGKGCEKCAFTGFYGRTGIF
                     ELLVIDDEIRKLILTNADANQIRLAARQRGMRTLLEDGVKKIKTGVTTLSEVFRVTQE
                     A"
     gene            6698..7891
                     /locus_tag="PK125_07365"
     CDS             6698..7891
                     /locus_tag="PK125_07365"
                     /inference="COORDINATES: protein motif:HMM:NF012694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HPB37963.1"
                     /translation="MAIFSYTATTMEGIMVEGVIEAPSQDVAIDRLKNSGVIPIKVAA
                     PRQSLGKRLSWRSSKADLLTFTTELSVLLNAGLPLDRGLNVLSETSDSKEMKGIVQSI
                     LKSIREGSSFSEALKKHPKVFPTLYVNMIRAGEAGGVLATVLDKLNEFLESTKELKDN
                     VVSAMIYPAILGITGGFSIIILLTFVLPKFTAIFSEIGGSLPLTTQILLAISNGFRSF
                     WWIGLSILIVVFLVLRSYVRSETGRYKWDVLKLKMMEDVIRKLETARFCRTLGTLLRS
                     GVSVLAALNNSKDVISNQVIASQIDAVLKGVKEGKGIAGPLSEAKVLPPLALSMMKVG
                     EETGQLDSMLIKVANAYEKSLREAVKRFMGILEPAIILGMGLIIGFIVISMLVAIFSM
                     TDLPF"
     gene            8026..8391
                     /locus_tag="PK125_07370"
     CDS             8026..8391
                     /locus_tag="PK125_07370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB37964.1"
                     /translation="MATLFFASTIPSAKLNGLGREISSMLKQARIVAQNRGEKQIMII
                     DLDSRTYGIEGKETKNIPPEITARVIDPLSGTVTQGKYSFVFYGSGGIEGATILLTYN
                     KKTLSIETDPVVGSIVVKQ"
     gene            8409..8846
                     /locus_tag="PK125_07375"
     CDS             8409..8846
                     /locus_tag="PK125_07375"
                     /inference="COORDINATES: protein motif:HMM:NF019575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HPB37965.1"
                     /translation="MRTRKTGPGRKVTRNHNPLSSRNGFTLLEILVALAILATAVTIM
                     FQLFSASLRNIAVSEDVVAASLKAEAKMREVLSKEELVVDSWEEQTDDGYRFVVNITD
                     TLQQKTDSLPMQLLQVDVAITWTKNSKERSLRLKTYKTVHRQA"
     gene            8848..9492
                     /locus_tag="PK125_07380"
     CDS             8848..9492
                     /locus_tag="PK125_07380"
                     /inference="COORDINATES: protein motif:HMM:NF019575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HPB37966.1"
                     /translation="MGLAEKTERNSGFTLLELIISITLVAIIVLIVAGAASLGYRSFS
                     SGEKKLNEIERLRASLIIIDAQIQSGVPLTLEDGGVKQYYFVGEKDSLKFSTNHSIWG
                     GQKGYVIVSYRVETDDQGKQRLIASEYKVGMENQKETRLLEGFKEITFDYYRQDAVDE
                     EGEWVTQWEDEEMMPTRIRLNLLWGANSLSYIMPVRVGGLIGELEDDDETEESE"
     gene            9532..10527
                     /locus_tag="PK125_07385"
     CDS             9532..10527
                     /locus_tag="PK125_07385"
                     /inference="COORDINATES: protein motif:HMM:NF015867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspK"
                     /protein_id="HPB37967.1"
                     /translation="MKWQGTGRITSTLFSSEKGLALIMVLWVLTILTVLSLSFTFMAR
                     TEVHSTIAFRDGIEKKLLAEGGIERGIIEIFYRSANKNQVSAVEGSEIVRIDGRENEG
                     EVGEDKYSFSIADESGKININALTDANSIIFNNLMVNLGIPGETANTIVDSVLDWKDE
                     DNLRRLNGAEDSYYEALPVPYKAKNGNFDTIEELLLVRGVTPEILFGNGSHPGLANFL
                     TVYTKGSTINLNAAAKEVIAALPNMTSSMADKIVEFRETAELKGQQDVIAIIGDVFRT
                     FVPYVGFAESNIYTIEGKGMKKNEKKGYAIRAVVAIEANNQHSYLYYKSPSDVRK"
     gene            10524..12059
                     /locus_tag="PK125_07390"
     CDS             10524..12059
                     /locus_tag="PK125_07390"
                     /inference="COORDINATES: protein motif:HMM:NF022554.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilN domain-containing protein"
                     /protein_id="HPB37968.1"
                     /translation="MMNRFPIEKIQNTIADSKAKMQRLWLPFWGKLTYNLAEGILLPL
                     KALSVSIEKGGIAVVYGSRFISKIRIRGSRYYGAEEGKYLQPERFAKTVSLAIDELKA
                     KKIEITLSIPREWAIIRTVELPSTVKENLVSVISYELDRLTPLSSDDALYDFRVLGEK
                     EEKLTVMIWAARLNLVNPYIDALRKEGVVVKKVTVGLSNLSNLSQYLAKDSPQIFLTY
                     NRYGYQGGLVLNGNVIATSGGFFNGEHRISKTERLVSDVHGLIEKAKEQGVEPKILIY
                     PESREQMTLEDKLEIPAQTIGNREIRERFYNDQVDTPFSATGGLLESLWDKANPLDLL
                     SKGLQVAKKTPIVVPITLIIIIAGLTAIYMMVPLQRAEKTLQEIERQINVRKEEVRKI
                     ESLKKEIDTLSDDVETIKRFKDARPTTLFLFRELTNILPKTVWLTRLRITDASVDIEG
                     YANSATEIISKLEASSHFRKVEFASPTIRDTRLNADRFVIKMELEGVLKAESENLNDG
                     KKK"
     gene            12040..12597
                     /gene="gspM"
                     /locus_tag="PK125_07395"
     CDS             12040..12597
                     /gene="gspM"
                     /locus_tag="PK125_07395"
                     /inference="COORDINATES: protein motif:HMM:NF040576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspM"
                     /protein_id="HPB37969.1"
                     /translation="MMEKRNRILIIAVPLMTILLGLLVYDYGYLKIREQLNSVKEKEA
                     FRTKTLTRYITLIAEKPQLEKQLTSLKERRKIDDSKLIEGQTLSLSAATLQENVKTII
                     TGKGGTISSERVDKPEDLGSFKLVTISIDSVLPDTRVLSDVLYAIETRTPYFVVKEID
                     ARVRNFREPRELMVKLKVSALTIGK"
     gene            12599..13315
                     /locus_tag="PK125_07400"
     CDS             12599..13315
                     /locus_tag="PK125_07400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB37970.1"
                     /translation="MKFGRYIANNVNLLNIILVVCVIVMSMAALSPYFYPQVRYNPPV
                     VKIPPAAEENQSEATAASPSLSDYMVIAEQNLFHPDRKIPLEKQDAQQMPKPELILYG
                     TLVTESMSVAYVEDKKNPKTSPGRGKRQNVMKKGDSVGGFVLKEIESDRIVLARGDET
                     MVVHLNQGDKPRISDIPSAPAVSAKPVPGAVPPPKIGTPMPVRPTTPHSAATPTPSPK
                     TDPMRAPSPMPRGSRFSPQK"
     gene            complement(13464..15773)
                     /locus_tag="PK125_07405"
     CDS             complement(13464..15773)
                     /locus_tag="PK125_07405"
                     /inference="COORDINATES: protein motif:HMM:NF012485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretin N-terminal domain-containing protein"
                     /protein_id="HPB37971.1"
                     /translation="MTTSSKIILLLIASVFLFISVIVSFSTGNAQQDVRFQDGLHGKY
                     IIAQAPKLLGQKQAPQPQVTKSKQDSTSRKKPLRAKAVADDDDDDEDEDDDDDDTIQP
                     LKDPKAISTTSQKPVSKPPLPSTPQATPGQVSAKPPVQQPQQTSPAPVVPTASSEGRP
                     TTPQSQNAQQQARPQPFIPPRPAPSRGSISLNFDDADVYQVIQTIFGDVLRANYIVDP
                     RVKGRVTFRSVAPIPTDQVLPVMETILRINAIGVVEDGGLYRIVPISEVAREPAPVGF
                     GRDPEKIPSTGKSLVQVVPVLFLQSSEVIKLITPFLSTNAVVIDVPKTNQIIIVDTDA
                     SVRRILQFLGTFDNEQQKKKQAQVYVYAVQNGKAKNVANLLQQIFLGARPATTSERPQ
                     TTTATTTPRPPTQQATQAPPPQQQLTKPPTTGTGETLVSDMTRIFADEILNSVIILAT
                     PEDYKLIKETIDKIDIQPRQVMIEGIVAQINLTDNLSLGLSYAFKTQFKISPDSVMQG
                     TLGLNTGKLGVESLPTSGFTFVGTVGDDYRAVINALATQSRAKVIAAPHILVTDNREA
                     RIQVGQQVPIVTSTSYDTTVTSSRTSTIQYKDIGIILKVTPQVNESGLVALDLAQEVS
                     TYSTIELGLNETTIILNKAEATTNVVVKDGETIIIGGLIREDTDKSRSGVPWLSKIPL
                     FGYLFGNTTDNETRQEYIILLTPRVIKNQNDAKEMTNDYVDRMTKSGKGKITTKELFG
                     TGRQGTVKKDLQTQPNVNQEPQRPQELSK"
     gene            complement(15770..16192)
                     /gene="gspG"
                     /locus_tag="PK125_07410"
     CDS             complement(15770..16192)
                     /gene="gspG"
                     /locus_tag="PK125_07410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006751354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system major pseudopilin GspG"
                     /protein_id="HPB37972.1"
                     /translation="MNQLIRKNKKGFTLIELLVVMVIVGLLAALVGPRLFPKLGKGKQ
                     AAAKAQIALLGQALDQFRLDVGRYPTTQEGLNALVTNPGIENWEGPYLTKGLPNDPWG
                     KAYHFQSPGSHGEYDLYSYGRDGTQGGEGEDKDVLSWE"
     gene            complement(16329..>17424)
                     /locus_tag="PK125_07415"
     CDS             complement(16329..>17424)
                     /locus_tag="PK125_07415"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF006761.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="pyruvate carboxylase subunit B"
                     /protein_id="HPB37973.1"
                     /translation="DDVVDAFVKKASEVGIDIFRVFDALNDERNFLTSFKAIRKNKKH
                     IQGAISYSLTLKEKKLGGPIFNIDYYIEKAKIIEDMGADSLCIKDMSGIISPYDAYKL
                     VSSLKANIKIPIHLHTHYTSGMASMSLLKAIEAGIDGIDTCLAPFSHRSSHPALEPFI
                     ATLKDTPHQLNFDLQTIADIDDYLEGIIPKYMTFADNTKYSVIDIGVLMHQIPGGMIS
                     NLISQLKQVKALHRLKDVLEEVPRTRMEFGFPPLVTPTSQIVGVQAVFNVLQGRYKMI
                     SSEVKDYFFGLYGRPPAPVDPEIRKKILKGYDRGEIPIDVRPGDILEPELPKARETLK
                     GISEDMGDILIYALYPMTGMEFLKKKYGIS"
BASE COUNT         5230 a         3259 c         4048 g         4887 t
ORIGIN      
        1 gttctgcgca tgatagccgg tgtatctatg gtgccagctc ccttttggat gaggcaaagg
       61 attcagaaat gcggaatgag gcccattaac gcagttgttg atgtgaccaa ctatgtaatg
      121 cttgagctcg gacaacccct ccatgcgttc gattatcatt ctttgcgaga aaaacgtata
      181 gaagtcagag ttgcaaacga aaatatagtg ttcaagaccc ttgacggtga agatagaaat
      241 atccttaaag gggatatcct gatatgtgat ggatcaggcc cagttgccat agccggtata
      301 atgggcggtg agaattcaga aatcacggaa aaaacaaggg atattgccct agagagtgca
      361 ttttttaacc cgttgtccat tcgacaaact gcaagaagaa ttggtattcg gtctgaagcc
      421 tctttaagat ttgaaaaggg cattgatctc gacaacgttg attttgcggc tgaaagagcc
      481 atatacctta tgaatttgac ggcaggtggg gttattgtaa atggaaagca agaggtatat
      541 gacaaaaaaa gcagtaagag catattcgtg aactttggaa agattatcga tatcctcgga
      601 acccctatag aacacaatgc aatcattagt tcattaagat ctattgacct tcatatagtc
      661 aaggaagagg agacaggttt tgttgtttcc gtgccgaact ttcgccatga tctcgatgaa
      721 tacatggata tcattgaaga agtggcacgc atatacgggt atgatcgtat acctgccaca
      781 accccagtag gttcttttca ggtgccaaaa aacgagaagc ggagtgccta ttttagcagt
      841 gccaaggagt attttactgc ttgtggattt catgagctga taaattttgc ttttttcagt
      901 gtaaaagata ttgaaaattt cttaataaat tcctccgatg aaagattctc aggtatacca
      961 attatgaatc ccatatcgaa agattacgag attatgcgga catttcttgc accaaatgtt
     1021 ctgaaaacca ttgcgtacaa tttaaaccgg ggtgccagaa acctgcaatt ttttgaaatg
     1081 ggaaaggttt ttgttgccgg tcatgatact atgccactgg aatatccttc catatgtttt
     1141 gtaatgacag gaaaagagag ggattacttt tggcgagatt cgtgcgcgga gtttgatttt
     1201 tttgacacta aaggtgttct tgagggtttt ggggccagat tcagagttca atttgatttt
     1261 gtgagaagca gtgagccctt tctcatgaag aacagatctg ctgatgttct gttggagggt
     1321 atcaaaatag gatgggttgg tgagatcgaa gacagtgtgt tacatactta taatattgaa
     1381 caaaaagtct attccgcaga actgagattt gatataatga tggaaaagag caaaataaat
     1441 gtacagtaca agcctatacc acgttatcct caggtaacac gagattttgc ttttcttgtg
     1501 gaaggggcgg taccggttgc gacaatgctt gagcagatca agactgtctc tccacttata
     1561 acagcggttg gcgtgtttga catgtttaag aaagaaacga agagtatagc actgagggtt
     1621 atttttcaat cttttgagga aacgttgact gatgacgcgg ttaatgccct gcaagagatc
     1681 atcatccaaa aggttacaag tataccagga gttacgctta gaacttaagg aggatttatg
     1741 acaaaattag agattgtaaa cgtgttgtat gaaaagcttg gattttcaaa gagggaatgc
     1801 gcacatatcg tggatacatt cttcgaggta ataaaaagca cactttcaaa aggagagaat
     1861 gtaaaactct caggatttgg taattttgtt gtaaaagaga aaaaatctag acgtggtagg
     1921 aacccgcaga cgggtaagga aatagagatt tctcaaagaa gagtggtcaa ctttaggcta
     1981 agccaggtct taaaggacgc tataaacaat aaaaacagaa aatgacctca aatatccctg
     2041 acagggtatt ttacaggatt aaagaggtgt gtaacctgac aggattgaag ccccatgtgt
     2101 tgagatactg ggagcaggag ttcaaggata taaaacctca aaaaagttcg aaggggcaaa
     2161 gattatataa gcggaaagat ctggaaacaa tttttaccat taaaaaactc ctctacgaaa
     2221 aacgctttac catagatggt gcaaaaaaat atatgtcgag ccagaaacat cttgtggacc
     2281 aaattaaaga ggatttgctg gaagtcgtga agattctgca aaaaggaggg tgatggtgaa
     2341 gaaatatatt attcttttca tggtggtgtt tttcctaagt gcctgtgccc atgggcctgt
     2401 gtatacagca aaaaatgaat cagacgcaat taagaaaaat taccataaca gtagacttca
     2461 ggcgatttat aatcagaatc agggtcttct gagcgagctt tacacaagat tcacggcgtc
     2521 gagaatagat atttataagg aagggcttgg cttcaccgac tttacaaacc aaaaggatga
     2581 aaaaatgtat tatctgatgg ttaaggtcag gccccaggag atcacatata aagagaaaac
     2641 aagcaattcc gatcaacggt tttcggaagc gctgcagact tacgcaccaa agtatttgaa
     2701 gtatctgaaa aattcagacc ttaatagaca atccatagat ggcattacgt ttggtgttta
     2761 ttgggctgtt cgggatgtat gtgacacata cggtggtttt attgagtacg ttgaatttta
     2821 cattccaaaa cagtttataa atgaatttct tgatggaaag attacttttc aagaaatgct
     2881 tctcgataca gaggtgctaa cgagcttaga caaacgtcac ccaacaaacg ttaaacctgt
     2941 gtttaagtag tctgttccta atacatctct cagatattcc cttaagtgga tcaatgtatt
     3001 tgatcccagc tttctataaa gcagtcaaat aaatgtgagt cattactaaa cagggatgcc
     3061 agtgttttca tttgtaatgg tgagcccagg aaatggagac accgtgaatg aaagaagaaa
     3121 aaccagaaga ataataaggg cgaacagttt tcttttactg tcaagctcag tttccatatc
     3181 aaatggttcg ggatgacgca tgccgaatat gagaaggagt attaccagga gtagccagcc
     3241 aggattatag aaaatggtca gaatcgcaag gaggcaaatg gagaatgcaa aaatccattt
     3301 actcttgttg ccaaagacag catagacgat gtgtcctccg tcgagctggc caatgggtag
     3361 aagattgagg gcggtgacga aaaggcctac ccatcctgca tatgccaaag ggtggagaga
     3421 tagatcataa ccttcaggaa tacttccaat cagaattttt tggagaattt taaatagaag
     3481 tggatcgcca agtctgagat aagccatttc actagttaca aattgaatgg ttgacatttt
     3541 tattccgagg atgatgaaag gaatagaaac tatgaaaccc gacaaaggac cggcgattcc
     3601 gatatcaaaa agcgccttct tgttgatgat aatacctttc atcttgatga ttgctccaag
     3661 agtgccaaac ggaggaaagg gcaaaggtat gaaataagga agtgttgcag gcactccata
     3721 tttccgactc atgaaatagt ggcccatttc atgagcaaga aggatagaca tcacagaggc
     3781 gctgtaggcc agcccaccta taaaagaggt tgtaagaata gtgagtatga aaagtattcc
     3841 gtggattatt acacgcctaa taaatcacgc tccacatgga tttgatcaaa ccgcgtgcca
     3901 cgtttcatct tcacatcaga catggtgagg ttgttgaaga aagatataag agtttgcctc
     3961 ggtatctttc tgcctgtttt cgaaagtttg tgagcataaa aaggcttctg gtagggaaaa
     4021 taatgttttt tttccgatgt ttttatttct aggctgtcct tccatttact aagggttttt
     4081 acaaatgttc cgtagtcctg gttggttcga caaagttctt ccatgtcttt agcctgcaac
     4141 agtagccttg tctgtctcac aagatagctg tgaatcgcga gtatatgcac tccattctga
     4201 ataagattct caaaaacctt gagcactcct tttttatcta attttgcaaa ggtatcaaaa
     4261 agggatatca ggctatcttc ctgcatttca gtaacaattg catgtatatc tttagagtct
     4321 atgcttgatt tttcgccaat atagtttatg gctttcatta attcagattc gaggttagat
     4381 tcatctttga ttttctccac caaagcacgg aatgctttgt cagtcatctt tttaccatgc
     4441 tgtttcagaa aggcaagaac acgtttttta atgcgtgttt ccctgtcttt atccataatt
     4501 aacatctcgt gagagatttt tttaagataa tctgggggtg aagatgttgt ataaatgacg
     4561 atgtgaatcc gttcccgcaa tatgtcgagt tggtgcctga gttcatcaaa gatagccttg
     4621 tctggatcta ccagggtaag gacaagattt tcttcaagaa aaagtgcaga tgttttctct
     4681 ataatagtcg ccttaatgtc ttttccggac acaacaagga cccggggatt ttgactccat
     4741 ttattcccca gaatatggat gtcgctggca aggttctctt ttgttccaac agcaatgaag
     4801 atttctttca acggtctaac caaaggttta atcggtaggt agtctatcca ctgaaaaaat
     4861 cagtcagatt aagtagatta ctatagaaga gctaatttta caatacctgt ctatttggtg
     4921 aattaaggca aaataaaatt gttctgatca gaaaattacg gtaaaaagcc aggtccatga
     4981 acagcgatcc tgttttgagt ctcttttttc aaaaagaggt gatgtgctgt gaacaataaa
     5041 atggagtaat tgatggtata caaaaaataa attgacccat gtactaaatt ttcaaggctg
     5101 tctctggttg ctaaaagctc tctgtgatat aatgaagttc agatacatca aggagagtcc
     5161 ttattatatg aaaagtgatg agacactata cataaatccc gatgattttc caacagttcc
     5221 tttagtactg gatggagtgt cctcccgttt tatccgcgaa aataagatca ttcctattga
     5281 actgaagaac aatatcctca aggtgattct tgcaaatccc gatgacagag agattattga
     5341 agcgttgaga gctactttat ctgctgatgt aattgtctat accggtgata gccagaccat
     5401 agatgaatat attgcaaggt tttatggcca ggggcctcag aatataaaca gaattattga
     5461 ggatatggac cagcagggat ttgaatacgt tagggaggag gaggaagaag atgttggtca
     5521 cctaaaagac ctcgcttcag aagctcccat tataaaactc gtaaatctcc tcatatcaag
     5581 agctgtcgat agcagggcta gcgatataca catagaaccg tttgaagatg aattgaaagt
     5641 ccgatacaga atagacggcg ttcttcatga tgtggaatct gcaccaaaaa aacttcaggc
     5701 tgcagttgtt tcaaggatca aaataatggc aaagttgaat attgctgaac gacggttgcc
     5761 acaggacggg cggattcggt tgaaatttgg ggaaagagag attgaccttc gtgtctcaac
     5821 aataccaatt cttcatggcg aaggcgtggt catgaggatc ctcgataagg agagcatagt
     5881 catagatctt gatacactcg gtttttttcc ggataccctt gcaagcttca ataagatcat
     5941 tacaaagccg aatgggatct ttctcgttac cggtcctaca gggagtggaa agacaacaac
     6001 actgtacgga gccctcgaca agatcaactc tcctgacaaa aaaattatta ctgtagaaga
     6061 tccaatcgaa tatcaattga agggtgtgaa tcagattcag gttaaaactc agattggtct
     6121 aaactttgct actacattac gacacattgt tcgccaagac cctgatatta ttatgatcgg
     6181 ggagatacga gatctcgaga cagcagaaat cgctattcag tcggccttga caggacattt
     6241 ggtcttttcg actcttcaca caaatgatgc cccgagcgct gtaacgcgtt taatcgatat
     6301 gggggttgag aacttcctcc tttcttcgac tgttaggggc atcctcgccc agaggctttt
     6361 gaggaatatt tgtcccggat gtaaggaggt agataaaacc cgggctaacc aagaggaatt
     6421 cagtgccctt ggtatgacag gagatattcc tttgtataag gggaaaggat gtgaaaagtg
     6481 tgcatttaca ggtttttatg gtcgaacagg tatctttgaa ttactcgtga tcgatgatga
     6541 gatacgcaag ctgatcctca ctaatgctga tgcaaaccag ataagactcg cagcaagaca
     6601 acgaggaatg aggactctat tggaggacgg tgtaaagaag ataaaaaccg gagttactac
     6661 gttgagtgaa gttttcagag tgacccagga ggcttaggtg gccatatttt cctatacggc
     6721 aaccacaatg gaaggcataa tggttgaggg tgtaatagaa gccccaagcc aggatgttgc
     6781 tattgacagg cttaaaaatt ctggtgtcat tccaattaaa gtagcggcgc caagacaaag
     6841 tctggggaag aggctaagct ggaggtcttc aaaggctgat ctgctgacat ttactacaga
     6901 actcagcgtt cttctcaatg ctggcctacc ccttgatcgc ggcctcaatg tcttatctga
     6961 gacatctgac agcaaggaga tgaaggggat tgtacaatct atcctcaaat caatacgcga
     7021 aggcagttcg ttttcggaag cgctcaaaaa acatcctaag gtatttccaa ctctctatgt
     7081 caatatgatc agggctggag aggccggagg tgtgcttgct acagtgcttg acaaacttaa
     7141 tgagttcctt gaatcgacaa aagagctgaa agacaatgtt gtctctgcca tgatatatcc
     7201 agctatcctc ggcataacgg gtggcttttc aatcattatc ctgttgacct ttgtattgcc
     7261 taaatttacc gcaatttttt cagaaatagg gggttcttta cccctcacaa cacaaatctt
     7321 acttgctata agcaatggct tcagatcgtt ctggtggatt ggcctttcca ttctgatcgt
     7381 tgtgttctta gtattaagga gctatgttcg ttctgaaact ggaaggtata aatgggatgt
     7441 tctgaaactg aaaatgatgg aagatgtgat tcgaaagctt gagactgccc gtttttgtag
     7501 gactctcggt acccttctca gaagtggtgt ttctgtgctt gctgcactca ataactcaaa
     7561 agacgttatt agcaaccagg taattgcttc acaaattgat gctgttttga aaggtgtaaa
     7621 agaagggaaa ggcattgccg gtccattatc agaagccaaa gtgttaccgc cgcttgctct
     7681 ttctatgatg aaggtcgggg aagagacagg gcaattggac tcgatgctta ttaaggttgc
     7741 aaatgcctat gagaaaagcc ttcgggaggc tgtgaagaga tttatgggta ttcttgaacc
     7801 ggcaattatt cttggaatgg gactcataat tggcttcatt gtaatatcaa tgctggtggc
     7861 tattttcagc atgactgatt tgcctttctg aagtataaaa gtggaggttc gtacatccct
     7921 cgaaagacac catacaagaa tcaaggtctg ggacttaata tttcacatgg atttactctt
     7981 cttgaattaa ttattgttat tgtaattatt gtccttgcct tgggtatggc cacacttttt
     8041 tttgccagta caatcccctc tgcaaaactg aatggtttag gcagagagat atcttcaatg
     8101 ctcaaacagg caagaattgt cgctcaaaat agaggggaaa agcaaatcat gatcattgat
     8161 cttgattcca gaacctacgg tatagagggt aaagagacaa aaaacattcc acccgaaata
     8221 actgcgagag tgatcgaccc tctgtccggc acagtcactc agggcaagta ttcttttgtc
     8281 ttttatgggt caggaggtat tgagggggcg acaattcttt tgacatataa caaaaaaacc
     8341 ctttctattg agacggaccc tgtagtaggg tcgatagtag tgaaacagtg aagtgcaaaa
     8401 agatagaaat gaggacaaga aagacgggac cagggagaaa agtgacaaga aaccataacc
     8461 cgttaagttc gaggaatgga tttacgcttt tggagattct tgttgccctg gcaattttag
     8521 ccactgcagt aaccatcatg ttccagcttt tctccgccag cctcagaaat attgcagtct
     8581 ctgaagatgt cgttgctgca tctctgaagg cggaggccaa aatgagagag gttttaagta
     8641 aagaagaact cgtggttgat tcttgggagg aacaaacaga tgacggatat agatttgtag
     8701 tgaacatcac tgacactctt cagcaaaaaa ctgatagcct tcccatgcaa ttgttgcagg
     8761 ttgatgttgc aatcacatgg accaagaatt caaaagaacg atcgctccgc ttaaaaacat
     8821 ataaaacagt gcacagacag gcatagaatg ggactggcag agaaaacaga acgcaatagc
     8881 ggatttaccc tgctggaact tatcatttcc attactctcg tcgccatcat tgtgcttatt
     8941 gtagctggag ctgcaagcct gggttacagg tcgttcagct caggagaaaa aaagctgaac
     9001 gagattgaaa gactgagggc atccctgatc attatagatg cccaaattca gtctggtgtg
     9061 ccgttgacat tggaagatgg aggtgttaaa caatactatt ttgttggaga aaaagattcg
     9121 ctgaagtttt ctacaaatca ttcaatctgg ggtggccaga aaggctatgt tattgtaagt
     9181 tacagggtag agacagatga tcagggaaag cagagactga ttgcatcaga atataaagta
     9241 ggtatggaga atcagaaaga gacaaggctt cttgagggtt tcaaggaaat cacctttgat
     9301 tattacagac aagatgctgt tgatgaagag ggggaatggg ttacgcagtg ggaagatgag
     9361 gaaatgatgc ctacaagaat aagactcaac cttttatggg gtgccaactc tttatcctat
     9421 ataatgcctg ttagagttgg aggattgatc ggagagcttg aggatgacga tgagacagaa
     9481 gagtcggaat gattgtacgc gggttttcat agataagcgg gggagatttc tgtgaaatgg
     9541 caaggaacag ggaggataac atcgacgctt ttctcttcag aaaaagggct ggccctgata
     9601 atggtacttt gggttcttac tatactcact gtcctgtctc tttcctttac gtttatggcg
     9661 cgaacagaag ttcactcaac cattgctttt cgtgatggga ttgaaaagaa gttacttgca
     9721 gaaggaggca tagaaagagg cattatagag atcttctaca gaagcgccaa taagaaccag
     9781 gttagtgctg tggaaggtag tgaaatagta cgtattgacg ggagggaaaa tgaaggtgaa
     9841 gttggtgaag ataaatactc attcagtata gccgatgagt caggaaagat caatattaat
     9901 gccctgactg atgccaacag catcattttc aataatttga tggtcaatct gggaatccca
     9961 ggagaaactg ccaatacgat tgttgattct gttcttgact ggaaggacga ggataacctc
    10021 cggaggctca acggggcaga agattcgtat tatgaagctc ttcctgttcc ttacaaggct
    10081 aaaaatggca attttgacac catcgaagag ctacttcttg ttcggggtgt aacaccggag
    10141 atactgttcg gcaatgggag tcatccaggt cttgccaatt ttcttaccgt atatacaaaa
    10201 ggatcgacta tcaatctgaa cgctgctgct aaagaggtta ttgcggccct tcctaacatg
    10261 acgtcctcaa tggcagataa aatcgttgag ttcagagaaa cagccgagtt gaaaggacaa
    10321 caggacgtaa tagctattat cggagatgtc ttcagaacat ttgtgccata tgttggcttt
    10381 gcagaatcaa atatatatac tattgaaggc aaaggcatga aaaagaatga aaaaaaaggg
    10441 tatgcaataa gagcagtagt agcaattgaa gcgaataacc aacatagcta cctttactat
    10501 aaaagtccgt cggatgttcg taaatgatga atagattccc catagaaaaa attcagaaca
    10561 ctattgctga ttccaaggca aaaatgcaac gcctctggct acctttttgg ggcaagctca
    10621 cctacaatct tgctgaggga attttgttac ccctaaaagc cctttcagtc tctatagaaa
    10681 agggtgggat tgcggttgtc tacggttcac gtttcatatc gaaaataaga atacgaggtt
    10741 cgcgttatta cggagcggaa gaagggaaat atcttcaacc cgagcgtttt gcaaagacag
    10801 tcagtctggc aatagatgag ttgaaagcaa aaaagattga aattacccta agtattccaa
    10861 gggaatgggc aataataaga acagttgaac ttccatctac tgttaaggag aatcttgttt
    10921 cggttatctc ctacgaactt gatcgtctca cccctctgag ttcggatgat gccctgtatg
    10981 attttagagt acttggagaa aaagaagaaa agctgaccgt tatgatatgg gcggcgagac
    11041 tcaatctcgt gaacccgtat atagacgctc tccgcaaaga aggggttgtt gttaaaaagg
    11101 ttactgtagg gctgtcgaac ttaagcaatc tttcgcaata tttagccaaa gactcacctc
    11161 agatttttct cacttacaat agatatggat accagggggg gcttgtttta aatggtaacg
    11221 taattgccac atcgggaggt ttttttaatg gtgaacatcg gatctcaaag accgaaagac
    11281 tcgtttctga tgttcatggg ttgatcgaga aagcaaaaga gcagggggtt gaaccaaaga
    11341 ttctcatata tcctgaaagc cgtgaacaga tgacattaga ggataaactg gagattcctg
    11401 cgcagacaat agggaataga gaaatacggg aaagatttta taatgaccaa gtagataccc
    11461 cttttagcgc aacaggaggg ttgcttgagt ctttatggga taaagcaaat cctttggatc
    11521 ttctcagtaa aggtctgcaa gttgcaaaaa agacgccaat tgtggtcccc ataacactta
    11581 tcatcataat tgcaggcctg acagcgattt atatgatggt cccccttcag agagcagaaa
    11641 aaactcttca agagattgaa agacagatta atgttagaaa agaggaggtt cgtaaaatag
    11701 aatcactaaa aaaagagatt gatacactga gtgatgacgt ggagacaatc aaacgattca
    11761 aagatgcaag acctacaact cttttcctct tcagggagtt gactaatata ctccccaaaa
    11821 cagtatggct gacgagatta agaatcactg acgcgagtgt agacatagaa ggttatgcga
    11881 attctgcaac tgagattatt tcaaaactcg aagcatcaag ccatttcagg aaggtagaat
    11941 ttgcatcacc aaccatacgc gatacccgat taaatgctga caggtttgtc attaaaatgg
    12001 agttggaagg tgttctgaaa gcagagagtg aaaatctgaa tgatggaaaa aagaaataga
    12061 atactcataa tagctgtacc tctcatgaca attctgctcg gtctgttggt atatgactat
    12121 ggctatctta agattaggga acagttgaac tcggtaaaag agaaagaggc atttagaaca
    12181 aaaaccctca caagatacat tacattgatt gcagaaaagc cccaactaga gaagcaattg
    12241 accagtctta aagagaggag aaagatagat gattcaaaac tcattgaggg gcagacactt
    12301 tcgctctcgg cagcaacact tcaggagaac gtaaaaacaa taatcaccgg taaaggtgga
    12361 actatttcga gtgagcgggt cgataaacca gaagatctag gaagttttaa gcttgtcact
    12421 atcagtattg attcagtgct gccagacaca agggtgttaa gtgacgtact ctatgctata
    12481 gaaactcgaa caccgtattt tgttgttaaa gaaatagatg cccgggtgag aaatttcaga
    12541 gagccaagag aattgatggt gaaactgaag gtttctgcct taactattgg taaatgacat
    12601 gaaatttggc cgatatatcg ccaacaacgt aaacctgctg aatatcatcc ttgttgtgtg
    12661 cgtcatagtg atgtcgatgg ctgcattatc tccttatttt tatcctcagg ttaggtataa
    12721 tcctccagta gtaaaaatac cacctgcagc tgaggagaat cagtccgaag caacggctgc
    12781 aagtccatcc ctttctgatt acatggttat tgcagagcaa aacctttttc acccggatcg
    12841 taagataccc cttgaaaaac aagacgcaca acagatgccc aagccagaac ttatcctcta
    12901 tggaactctt gtaaccgaga gcatgtctgt tgcttatgtt gaggacaaaa aaaatccaaa
    12961 gacaagccct gggcgtggca agcggcaaaa tgttatgaag aagggagatt cggtgggagg
    13021 ttttgttctt aaagagattg agtcagaccg gatagtcctt gcaaggggtg atgaaacaat
    13081 ggttgtccac ctcaatcaag gagacaagcc aaggataagc gatattccaa gtgcgcctgc
    13141 tgttagtgcc aagccagttc ctggcgcagt ccccccgccg aagattggaa cacccatgcc
    13201 ggtcaggccg acaacccctc attctgctgc aacaccaact ccgtctccaa aaacagatcc
    13261 aatgagggcg ccttctccaa tgccaagagg gtctagattc agccctcaaa aataaaacct
    13321 tcagagtcag taaaagaaat ataggaatat atccaaatgt aaagaatact gtaaggaatg
    13381 cgttttactg tcatacagga gatatgcttt tttgccactg tttggcacag gtatgtttag
    13441 tgccagcaat cgtcagaacg ttgttatttt gagagttctt gaggtctttg tggctcttga
    13501 tttacatttg gttgggtttg caagtctttt ttcacggtgc cttgcctgcc tgtcccaaaa
    13561 agctctttag tcgttatctt ccctttgcct gatttggtca tcctatcgac ataatcattt
    13621 gtcatttctt ttgcatcatt ttggttcttt atgacgcggg gtgtcaggag aatgatatac
    13681 tcctggcgtg tttcattgtc agtagtattg ccaaaaaggt agccaaagag aggtattttg
    13741 gacaaccagg gaactcctga tctggattta tccgtgtcct ccctgataag gcctcctatg
    13801 ataatcgtct caccgtcctt caccacaaca tttgttgttg cttcggcttt gttgagaata
    13861 atagttgttt cattgaggcc aagttcaatg gtgctgtatg ttgaaacctc ctgagccagg
    13921 tcaagagcaa cgagaccact ctcattcact tggggtgtta cctttaaaat aattccaata
    13981 tccttatact ggattgtgct cgtacggctg gaagttactg tggtatcata tgatgtagag
    14041 gtcacaattg gaacctgctg gcctacctga atccgtgctt ctcgattatc tgttacaagg
    14101 atatggggag ctgctataac ctttgctctt gactgggttg caagggcgtt aatgactgcc
    14161 cgatagtcat caccaacagt acccacgaaa gtaaaaccag aagttggaag cgattctacg
    14221 cccagcttgc ctgtatttaa accaagggtt ccctgcatta ccgagtctgg gcttatctta
    14281 aattgggttt tgaaggcgta ggacaaacca agactcaaat tatctgtaag attgatctgg
    14341 gctacgatac cttcaatcat gacctgtcga ggctgaatat ctattttgtc gattgtctct
    14401 ttgattaatt tataatcctc aggggttgcg aggattatga cagaattcag tatttcatcg
    14461 gcgaagatcc gggtcatgtc cgagaccagg gtttctccag taccagttgt gggtggttta
    14521 gtaagctgct gttggggtgg aggggcttgt gttgcctgct gggttggtgg tcttggcgtt
    14581 gtggtggctg tggttgtttg tggtcgctct gatgttgttg caggacgagc cccgaggaag
    14641 atctgctgta aaaggtttgc cacatttttg gccttcccgt tttgtaccgc ataaacgtaa
    14701 acttgagcct gtttcttttt ttgctgttca ttgtcgaagg tgcccaagaa ctggagtatt
    14761 cttctgacgc tggcatcggt gtcaactata ataatctggt tagtctttgg aacatcgata
    14821 acaaccgcgt ttgttgatag aaatggtgta ataagcttga tcacttcaga agactggagg
    14881 aatagaacag gcactacctg aacaagagat tttcctgtgg aaggaatttt ttccgggtct
    14941 ctcccgaaac caacaggagc aggttcgcgg gcgacctcac ttatcggtac aattctataa
    15001 agcccgccat cttcaacaac accaattgca ttgattctga gtatagtctc catgaccggc
    15061 agaacctgat cagtaggtat gggtgcaaca gatctaaaag tgactcggcc ttttacgcgc
    15121 ggatcaacga tatagttggc acgcagcaca tctccgaaaa tcgtctgtat tacttgatac
    15181 acatctgcat catcaaaatt gaggcttatt gaacctcttg aaggggctgg tcttggaggg
    15241 ataaatggtt gtggacgtgc ttgctgctga gcattttgag attgtggagt tgttggtcta
    15301 ccctcagaag aagcagtggg aacaacaggg gctggggatg tctgctgtgg ctgctgaacg
    15361 ggaggttttg ccgaaacctg tcccggtgtt gcttgtgggg ttgagggcaa aggaggttta
    15421 gacacaggtt tttgagaagt tgtagagatt gctttggggt cttttaacgg ctgaatagta
    15481 tcatcgtcat catcgtcctc atcctcgtca tcgtcatcat cgtctgcgac tgctttggct
    15541 cgtaaaggct tttttctaga agtagaatct tgtttcgatt tggtaacctg aggttgtggt
    15601 gcttgtttct gcccgagtaa ctttggggct tgagcaataa tgtatttgcc gtgcagacca
    15661 tcttgaaaac gaacatcttg ctgggcattg cctgtggaaa aagagacaat tacagagatg
    15721 aatagaaaca cagaagcaat caacaacagt ataatttttg atgaggtggt cattcccagc
    15781 tcaggacatc cttgtcttcg ccctcacccc cctgagttcc atctctacca tatgaataca
    15841 gatcatattc cccatgggag cccggcgatt ggaaatgata tgctttcccc cagggatcat
    15901 tgggcagtcc cttggtaagg taaggtcctt cccagttttc gattcctgga ttagtaacga
    15961 gggcatttaa accctcttgg gtagttgggt atcgtccaac atcgagtctg aactggtcaa
    16021 gagcttgacc aagtaatgct atttgggcct tggctgcagc ttgctttccc tttccaagct
    16081 tcgggaaaag ccttggcccg acaagggcgg caagcaatcc aacgattacc atgacaacga
    16141 ggagctcgat aagggtaaaa cctttcttat tttttctgat gagctgattc atggtcaggt
    16201 aacctccatt agacctacgt aattatactg aatagaatga aattggtcaa ataattatgt
    16261 caaattaaag tgccatttat tgggacaata agagatgttt tccttaacac gtaaaaggaa
    16321 cacttgtgtc atgatatgcc atactttttc ttcaggaatt ccataccagt catcgggtaa
    16381 agtgcataga tgaggatatc acccatgtct tccgaaattc ctttaagtgt ttcacgggcc
    16441 tttgggagtt caggctcaag gatgtcaccc ggtctcacat ctattggtat ttcacctcta
    16501 tcgtaaccct tgaggatttt ttttctaatc tcagggtcaa caggcgcagg gggtcggcca
    16561 taaaggccga aaaaataatc ttttacttca ctggaaatca ttttataacg tccctgaagg
    16621 acattaaaga cagcttgaac accaacaatc tgacttgtgg gggtgacaag aggtggaaag
    16681 ccaaattcca ttctagttct tggaacctct tcaaggacat cctttagtct atgcaatgct
    16741 tttacttgtt ttagttgact tatgaggttg ctaatcatac cgcctggaat ttggtgcatc
    16801 aaaactccga tgtctattac ggaatacttt gtgttgtcgg caaaagtcat gtatttagga
    16861 attatgcctt cgagataatc gtcaatatca gctatagttt gaagatcaaa attgagctga
    16921 tggggtgtgt ctttgagggt ggcgataaaa ggttccagtg ctgggtggga tgacctgtga
    16981 gaaaatggtg caaggcatgt gtcaatgcca tctatgccgg cttcgatagc cttgagcaat
    17041 gacatagatg ccataccact cgtataatgg gtatgcagat ggatgggaat tttaatatta
    17101 gcctttagtg atgacacgag cttgtaggca tcgtacggtg aaatgatacc ggacatgtcc
    17161 ttaatacaaa gggaatctgc ccccatatcc tcaataattt tagccttttc tatgtaataa
    17221 tcgatgttga aaatcggccc gccaagtttt ttttctttga gcgtgagaga gtatgaaata
    17281 gcaccttgaa tatgtttctt attttttcta atagctttaa aggatgtcag aaagttgcgc
    17341 tcatcattga gagcatcaaa gactctaaaa atgtcaatgc caacctcaga agcttttttt
    17401 acaaaagcat caactacatc atct
//
LOCUS       DAOOMC010000059        16774 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdus sp. isolate AMR_MDS_2685
            NODE_3602_length_16774_cov_6.835995, whole genome shotgun sequence.
ACCESSION   DAOOMC010000059 DAOOMC010000000
VERSION     DAOOMC010000059.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864760
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdus sp. (wastewater metagenome)
  ORGANISM  Syntrophorhabdus sp.
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae; Syntrophorhabdus.
REFERENCE   1  (bases 1 to 16774)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16774)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,729
            Genes (coding)                    :: 2,708
            CDSs (with protein)               :: 2,708
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 7 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16774
                     /organism="Syntrophorhabdus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3602_length_16774_cov_6.835995"
                     /isolate="AMR_MDS_2685"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2699756"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..777)
                     /gene="menA"
                     /locus_tag="PK125_07420"
     CDS             complement(<1..777)
                     /gene="menA"
                     /locus_tag="PK125_07420"
                     /inference="COORDINATES: protein motif:HMM:TIGR00751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046428 - 1,4-dihydroxy-2-naphthoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     octaprenyltransferase"
                     /protein_id="HPB37974.1"
                     /translation="MDKEKIKAWIQASRPPFFIATLIPIIIGFLMAKDHGWNGMRFLL
                     VLIACFVVHFVTNIANDYFDYIQGADSGNAIGGSRVIHQDKITLSQLGWAIILLYFIG
                     FIISLYIVFGLRLYGLAPLILFAFFSSLFYVAPPIRYGYHGLGELFVAVNMGPIMVVG
                     TYWVIADKPSWDAFFVSVPVGLMVASILYYQSLPDMGTDESAGKRTLAVRLGKQWSFI
                     VLILWLTIIYLTIIALVATGLLAWPGLLSLMTVPIFLRLIR"
     gene            complement(841..1602)
                     /gene="ubiE"
                     /locus_tag="PK125_07425"
     CDS             complement(841..1602)
                     /gene="ubiE"
                     /locus_tag="PK125_07425"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: protein motif:HMM:NF001244.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052624 - 2-phytyl-1,4-naphthoquinone
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0042372 - phylloquinone biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HPB37975.1"
                     /translation="MEKHINKVYPSVRDVTEKERVGMVKEIFSTITPRYDFLNHFLSL
                     RRDMAWRRFAIKKMHFFKTKQLLDIACGTGDLSVDAAQKYPDITVCGVDFVSEMIRAG
                     QKKINREGLTKRIQFMTGDALHLPFRDNTFDVAAMAFGIRNIPDRQGALREMRRVIVP
                     GGQIMILEMTFTRNRFFQIFYNIYLNYILPRLAKRFSSNPGAYHYLADSIMNFPTHDQ
                     FTALMEETGIENVDAYSLTFGVTYLHVGTKPACSL"
     gene            complement(1587..2336)
                     /locus_tag="PK125_07430"
     CDS             complement(1587..2336)
                     /locus_tag="PK125_07430"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPB37976.1"
                     /translation="MKKRQLLITYLVTIILIYGLWYVLSLLLGSNLLPDPFVVLRQGV
                     LELDKAPFWSHVGLSLYRILAGLFFGFILAVPIGLFLGSNERFDRLFAPLIYLGYPIP
                     KIVLMPIIFVLFGLGDMGKIVLITMIIFFQLLITTRDSARKISKEVIYSLQSLGGNRW
                     HLYRHVVWPISLPGIFTSLRIGTGTAVAVLFFVESIATRKGLGFYIIDAWGRADYSTM
                     FVGIIALSCIGIILYEVFDLLERKLCKWKNI"
     gene            complement(2333..3085)
                     /locus_tag="PK125_07435"
     CDS             complement(2333..3085)
                     /locus_tag="PK125_07435"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HPB37977.1"
                     /translation="MSNSLLCVESLSKYFPGLNKPKKVLEDVSFTLSSWDSLAIMGPS
                     GCGKTTLLLIIAGLLQSSRGSITIDGEPINGPTRKIGLVLQEYGLFPWKTVEANILLG
                     ARFQKLKIPEKRLSEFKSVLGIEGLDHLYPHQLSGGQRQRVALARALILRPALLLLDE
                     PFAAIDAITRERLQDRLLAVFGHRQFSFIIVTHNIEEAIFLGKRILILNNKACGLCTI
                     MENPGVGDLHYRTKPGFFELSTRLRNLLEGLD"
     gene            complement(3075..4046)
                     /locus_tag="PK125_07440"
     CDS             complement(3075..4046)
                     /locus_tag="PK125_07440"
                     /inference="COORDINATES: protein motif:HMM:NF024772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HPB37978.1"
                     /translation="MWSIKVCILLVATVFSMHGFWGCSPSSQDKGGKSPSVTIQFGTL
                     PVIQALPLFVAAEKGYFKDAGLDVELVPFNSAMEKDVAMSAGQISGYFGDMITPMVLT
                     ANGTLVKIVATHFNIKNEQRMFAILAGPKYPKKDLHALSSAGIACSSNTMLDYLMTRL
                     LQKKGIAANTINLVEVKSIPIRLQMLMSDQVPAAILPEPLVTLAEMKGARVMIDDRKT
                     GLTPTVLVFSDQFLAKNPDAVKAFLQAVQKGNDYINKNPDEVRPTMNRECKIPEPLHQ
                     SFAIPAFPKLNVPKNKEVMDVYSWLREKNVIKTDMTYGQFVAEGYVQ"
     gene            complement(4187..4420)
                     /locus_tag="PK125_07445"
     CDS             complement(4187..4420)
                     /locus_tag="PK125_07445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB37979.1"
                     /translation="MDDSLQEEEKKLRRLRFIVDFAIEYLRSQKLNHDEAIRVVEGVR
                     KHALKLFPGKEDAFDIIYAPRFKRVLNEKFQRS"
     gene            complement(4578..5114)
                     /gene="lspA"
                     /locus_tag="PK125_07450"
     CDS             complement(4578..5114)
                     /gene="lspA"
                     /locus_tag="PK125_07450"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: protein motif:HMM:NF013421.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HPB37980.1"
                     /translation="MRRYLVLLIIPCIFLLDRWTKLLIIQNLSYMEIIELAPFFSIVH
                     ARNLGGAFGILAGFGFAKHFFTYLPLLIILALVYILIVYRISMGKMLALTLILSGAVG
                     NVYERIFYGYVTDFLDFHYQNLHWPAFNVADIAISTGIGLWLFVELREMIRARRAGKE
                     VKVKSVKGEEKTKGQDRK"
     gene            complement(5087..7849)
                     /gene="ileS"
                     /locus_tag="PK125_07455"
     CDS             complement(5087..7849)
                     /gene="ileS"
                     /locus_tag="PK125_07455"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HPB37981.1"
                     /translation="MDYKNTLNLPKTPFPMKGNLPVKEKEMLKSWEDMKLYGKLVERA
                     ANHPLFILHDGPPYANGHIHLGTALNKILKDIIVKSKFMTGFRADYIPGWDCHGLPIE
                     HQVEKEMKEKKASLSKLDIRHRCRAYAESFIDVQREEFKRLGGIGLWEKPYITMDYRY
                     QATIIEQVQKFFARDEVYRMKKPVHWCMSCLTALAEAEIEYELKKSTSIYVKFPYTGS
                     YEGPFAAYPDKPIFMLIWTTTPWTLPANLAIAINPTFTYVAFDVGGEIYIALKELVEE
                     ITQKAGIGGYKILGEIPADSLMKLSFKHPFIERDSVMVYGDYVASDTGTGAVHTAPGH
                     GEEDYETGIEYGLDIYSPVNERGEFLDEVEFFRGMNVFDSNQHVIKKLQELGRLLYQE
                     EIEHSYPHCWRCKKPVIFRATEQWFISLDKNGLRQKALDEIDRVRWIPTWGRDRIFNM
                     LQVRPDWCISRQRTWGIPITIFYCEKCREPFWSNESFRNVIKAVEESGADIWFEKDAS
                     YFLPRGTTCKKCNNPTFVKEEDILDVWFDSGVSWAAVCKERKELTYPVDMYLEGSDQH
                     RGWFHSSLLTSVGNEGQAPYKSVLTHGFVVDGTGRKMSKSLGNVIAPEEIIEKYGAEI
                     LRLWATYEDYRDDIKISKDIINRLVETYRRVRNTLRFLHANIHDDFDPTQHSVSYDQL
                     SYLDKWLLSRLHRLIERVRDAYNTYTFHTIYHAVQNFCTVDLSALYLDIVKDRIYVEN
                     KNALKRRASQTVIYETLVSLLKLIAPILSCTAEEMWTFLKGYVQEESIFLASFPESRK
                     ELIRPEVEEEWEKIWRIRELANKKIEEKRVEKVVGHSLDTRIVIKASESDFALLGRLG
                     HELKDVFIVSQIDLQKGDSDTEITVSKADGQKCERCWQYATDITAAAGKFPNTCKRCA
                     DTLSSS"
     gene            8192..8617
                     /locus_tag="PK125_07460"
     CDS             8192..8617
                     /locus_tag="PK125_07460"
                     /EC_number="1.-.-.-"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: protein motif:HMM:NF013412.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine-phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HPB37982.1"
                     /translation="MTTKLMDYFNRMPRLGCLSTASKEGKVNVAYFGSPRMIDDKTIV
                     MGLGKNRTFFYLQENPYGVFMIMEPGKVLTEWKGVRLYVKMTECATSGSKYEEFKAQM
                     AKSAGEAAAKLIHAAVTFEVYEMKPLADFGQGWEKSIGV"
     gene            complement(8719..9054)
                     /gene="atpE"
                     /locus_tag="PK125_07465"
     CDS             complement(8719..9054)
                     /gene="atpE"
                     /locus_tag="PK125_07465"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF012365.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HPB37983.1"
                     /translation="MKKVLVVTMLLGVFISFSFVSIAMAAEGAATGGLDSGVKQMVAL
                     AAGLGIAIAAFGGAIGQGRSIASALDGIARNPGASGKITTPMIIGLAMIESLVIYSLV
                     VSLLLIFKL"
     gene            complement(9082..9774)
                     /gene="atpB"
                     /locus_tag="PK125_07470"
     CDS             complement(9082..9774)
                     /gene="atpB"
                     /locus_tag="PK125_07470"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HPB37984.1"
                     /translation="MGHDWSWAELFPILRDQPHVFNGIIVSAIILVIVIMGYKQLKKT
                     EDDIVPSPKLTFRNAVELIVESLANIVVDTMGPRGKEFVLIVGTLGLFILFNNLSGLV
                     PGFLPATDNVNTTFACSLTVFVLTHYYGFKEHGFKYLKQFVGPMWALSPLMIPVELIG
                     HIARPLSLGLRLFGNITGDHLVTAIFFGLVPILVPLPVMFLGLFVAFVQTFVFMLLSM
                     AYFSGAISHEEH"
     gene            complement(9764..10156)
                     /locus_tag="PK125_07475"
     CDS             complement(9764..10156)
                     /locus_tag="PK125_07475"
                     /inference="COORDINATES: protein motif:HMM:NF015834.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase subunit I"
                     /protein_id="HPB37985.1"
                     /translation="MKETKISDIERINIVVLVIGSILAIVVMREFKYVFSFAVASAIM
                     TLNFRFIKKIIEGGLLKPEGKKVELAIKLPIKFLILIALVAIVVIYGDIDVVFFLIGL
                     STVFISVVINQFSSLWNPALKRRQKNGA"
     gene            complement(10134..10409)
                     /locus_tag="PK125_07480"
     CDS             complement(10134..10409)
                     /locus_tag="PK125_07480"
                     /inference="COORDINATES: protein motif:HMM:NF021073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AtpZ/AtpI family protein"
                     /protein_id="HPB37986.1"
                     /translation="MINKSGLEMVKSLSDDKRDLVKSLISYSSLGLEMGLCVAIGIAI
                     GYFLDRYFMTSPYLTIIFMIFGIVAAMKSIYQLMKKLEKENERNKDK"
     gene            complement(10717..10974)
                     /locus_tag="PK125_07485"
     CDS             complement(10717..10974)
                     /locus_tag="PK125_07485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB37987.1"
                     /translation="MYFMNICTWSPEDEKEVARRRADWKWPKGVKVICEFIDLQGCRV
                     INVVDTDARGLIASRESWIDLMLFETFPVYPFGASKNLLKK"
     gene            11113..11186
                     /locus_tag="PK125_07490"
     tRNA            11113..11186
                     /locus_tag="PK125_07490"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11147..11149,aa:Lys,seq:ctt)
     gene            complement(11372..12496)
                     /locus_tag="PK125_07495"
     CDS             complement(11372..12496)
                     /locus_tag="PK125_07495"
                     /inference="COORDINATES: protein motif:HMM:NF024911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HPB37988.1"
                     /translation="MYKNSRLIEPVSMIVLTGGPCSGKSSSLAYLTERLSDNGFMVFV
                     IPETATLITNSGIDRRKMNKSKQVVMYEEAILDMQLAFEETYQQTVTRIFPERKKIIL
                     LDRGIMDIKAFMPDDDFRAILKKKRLTEVRLRDRYNGVIHLVTAAEGAPAFYTGENNS
                     ARIETPEEARIIDQKIRESWLGHPHFKIIDNSTDFEGKIARTFSAISHFLGLPAPLQI
                     SEKFLVKNVRHEKLPTHQIIHMEQMYLRSKNKNEEIRIRKRGQNRAYLYFLSRKTLVE
                     EEELITEQEYLNLTKLIDPKTDVIEKERICFLWSNQYCELDTYTGKYEGVIILKIEPV
                     ETTGDSANMQIPPFIDIEDNITGNLYYNERSMAMKQKKRT"
     gene            complement(12600..13154)
                     /locus_tag="PK125_07500"
     CDS             complement(12600..13154)
                     /locus_tag="PK125_07500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB37989.1"
                     /translation="MQIVKQKKESPVNFEDLLETFQAKKRITEPKEENRQHYKAFPLG
                     TVALFIMVIALGTMVIILKSDMAVLKNDIAHLREFRTQIATLDPKIQISHVESKFTDL
                     EKEEVKLKGEVNQFKIDLETMKTEQPRVVTNRVSKKLTKRPPPEQNRSSKIKSEDILK
                     KKIPHQDIYFSDISRATNPDPSTK"
     gene            complement(13359..14669)
                     /locus_tag="PK125_07505"
     CDS             complement(13359..14669)
                     /locus_tag="PK125_07505"
                     /inference="COORDINATES: protein motif:HMM:NF024793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HPB37990.1"
                     /translation="MANTVYGIGMKKGLMVIYGDVGVGKTMVAQALLAKSDREYLKTI
                     YIDNADITFLELLQAISRQFGLTTTDNISEMITELRQFLLDEDKADRTVALFIDNAHS
                     MPVETLDNLVVLSNLETKDKKLLQIVLLGQPKLETLLNKNELRHIKQRVAIRTTITPL
                     SPDESLSYVQHRLEKAGARDTSAFTKSALKRILREAKGIPRVINVLCDNALVTAFGRQ
                     TKSVTSEAANEAITDLMGSKQHAYFKKYLRWQTGAMAAVMCLCVVLLIAFRSGYFSPI
                     YSQKEVSIESNLQAANTSTSFTVSNQSAVPQTNPQSEQDKALQENVPEQSNTARLKEK
                     EVPQFPPKNTISKTDIMASKTQVDPQTAPKSVLLQTKIERGKRMVKVKVRQGDSFIML
                     IKDFYGRTDKGLLAFVKSKNSHIQDFHNLRQGTTLFFPSASDIP"
     gene            complement(14779..>16774)
                     /locus_tag="PK125_07510"
     CDS             complement(14779..>16774)
                     /locus_tag="PK125_07510"
                     /inference="COORDINATES: protein motif:HMM:NF019237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1566 domain-containing protein"
                     /protein_id="HPB37991.1"
                     /translation="GTIELPQTGQSTCYSNSGDIIPCDNTGQDGDFIAGVSWPMPRFQ
                     LDKSRACITDNLTGLMWSRQAWNLTSYTSWATSVLNPPTAFFQCGFSDWRLPNINELQ
                     SLVNFEEYNNVEWLTSQGFYFLNVLPKKYWSSTSDAISPEVNAWNMDMVDGSVVAEPK
                     TANGLIWPVRGTSNPDKNAPPGQIAETGQISIKATYDDAYYATTPGRNVGVPWPDPRF
                     TLTYCDATGPCIDQDADCDGEDINNIVKDNLTGLVWSANANAHGLKTWSDALAYTNNL
                     TTCGYGDWRLPNAKELFSIIDRSQGEPALPAKHHFINVQYGADDSYWASTSYAYDPDR
                     AWAIDLFDGALGPRAKTGTAFVWPVRGGRTKPYVLTVKKEGAGKGKITATDLTCVGAT
                     CTGYYNSYEEITVTATPSSGSVFFEWTDCPDPSGNTCTITMTHDITVKAIFLPEYKIY
                     VSPRSLNFKNLKQSVPSAPLTVTVKNAGVSPLQITSIGVTGDDASVFSLTPAHDCPAE
                     LTNLNDFCTVSVVASTDNYDARKAHLKISSNDPKKPVFTVNLRARAKPPKISKKPSTL
                     SFGKVNVGIPVNKILTLKNKGITDLVIGVITIAGDHPGDFSTLAADTCSSSTLVTNGT
                     CTVSVAFTPSVPEKRSAILQVPSNDPKRSLLSVNLKGTGG"
BASE COUNT         4470 a         3730 c         3799 g         4775 t
ORIGIN      
        1 acggattaac ctcaaaaaga tagggactgt catgagtgat aagagaccag gccatgccag
       61 aagtccggtt gcaacgaggg cgatgatggt aaggtaaata atagttagcc atagaattaa
      121 tacgatgaag gaccactgtt taccgagacg taccgcaaga gtcctcttgc cggctgactc
      181 atcagtaccc atatctggaa ggctttgata ataaaggata gaggccacca tcagaccaac
      241 aggaacagaa acaaaaaaag catcccaact tggcttgtca gcaataaccc aataggtccc
      301 cactaccatg ataggtccca tattgactgc aacaaaaagt tctccaaggc cgtgatagcc
      361 gtaacgaata ggaggtgcaa cgtaaaagag gctactgaaa aaggcaaaaa ggatgagtgg
      421 cgcaagaccg tagagccgga ggccaaacac aatatagaga gaaataataa acccgataaa
      481 atagagaagg atgattgccc aaccgagttg ggacagggtt attttatcct gatgaatcac
      541 tcttgacccg ccaatggcat tccctgagtc agcgccctgg atgtagtcaa agtaatcatt
      601 tgcaatgttt gtgacaaaat ggaccacaaa acaggcgata agaaccagga gaaatctcat
      661 cccattccat ccatggtctt ttgccataag aaatccaatg ataatcggga taagggtggc
      721 aataaaaaaa ggtggtcggc ttgcctgaat ccatgctttt attttctctt tatccatata
      781 tccccggaaa catgcttata gttgtaagct catggcaaat ccgaaatctt ttgcttagag
      841 ctataaacta caggctggtt tcgttcccac atgcaggtat gttacaccaa aggtgaggct
      901 gtatgcatca acattttcaa taccggtttc ctccattaga gcagtgaatt gatcatgagt
      961 gggaaagttc ataatggagt cggcaaggta atggtaggca ccagggtttg aagagaaacg
     1021 cttcgcaagg cggggcagaa tgtaattcag gtagatattg tagaatattt gaaagaaacg
     1081 attacgggta aaagtcatct caagaatcat gatttgcccc ccgggcacga ttacccgacg
     1141 catttccctc agagctccct gtctgtcagg aatatttcgg ataccgaagg ccatggcagc
     1201 cacatcgaat gtgttgtctc gaaaaggcag atggagagca tcacctgtca tgaactgtat
     1261 tcttttggtg agcccttccc ggttgatttt tttctggcct gctcgaatca tttctgaaac
     1321 aaaatcgacc ccacatacag taatatctgg atatttttgt gctgcatcca cagagaggtc
     1381 gccggtgccg catgcaatat cgagaagttg tttcgtcttg aagaagtgca ttttttttat
     1441 ggcaaaacgg cgccatgcca tatcccgcct caggctcagg aagtgattaa ggaaatcata
     1501 ccgtggagtt atagtggaaa atatctcctt taccatgccc acccgttctt tttcagtgac
     1561 atcacgaact gacggatata ccttgtttat atgtttttcc atttgcagag ctttctttcg
     1621 agaaggtcga aaacctcata cagaataatg ccaatgcagg ataaggcgat aatacctaca
     1681 aacatagtag aataatctgc acgcccccat gcgtcgatga tgtaaaagcc aagacctttc
     1741 cttgttgcaa tcgattcgac aaaaaagaga acagcgacag ctgtgcctgt tccaatccgc
     1801 agcgatgtaa agattccagg aaggcttatg ggccatacca cgtgccggta aaggtgccac
     1861 cggtttcccc ccagagattg taaggagtaa atgacttctt tactaatctt ccgcgcactg
     1921 tctcgtgttg tgataagcag ttgaaaaaag ataatcattg tgatgagtac aattttaccc
     1981 atatctccaa gaccaaagag cacgaagatt atgggcatga gaacaatctt ggggatagga
     2041 tacccgagat agatgagagg ggcgaaaagg cggtcaaagc gctcattgct tccgagaaaa
     2101 aggcctattg gcacagccag aataaatcca aaaaacagac cagcaaggat gcggtagaga
     2161 ctcaatccca cgtggctcca aaaaggggcc ttgtcaagct ccagaacgcc ttgccgaagg
     2221 acaacaaagg ggtctggaag cagattactg ccaagaagca gggaaagtac ataccagagc
     2281 ccataaataa ggatgatggt gacaaggtat gtgatgagaa gttgtctctt cttcaatcca
     2341 atccctcgag aaggtttctg agtcttgtgc tcagctcaaa gaaacccggt ttagtacgat
     2401 aatgtaaatc gccaacgcca ggattctcca taatggtgca caagccgcat gctttgttat
     2461 tcagtatgag aatccttttg ccaagaaaaa tggcctcctc tatgttatgg gtgacaatga
     2521 tgaagctaaa ttgtcggtga ccgaagacag ccaacaaccg gtcctgcaat cgttctctgg
     2581 tgatggcgtc aatcgcagca aaaggttcat cgagcaagag gagggcaggg cgcaggatca
     2641 aagctctcgc cagggcgacc cgttgacgtt ggccaccgct caactgatgt ggataaaggt
     2701 gatcgaggcc ttcaataccg aggacacttt taaactcaga aagccttttc tccgggatct
     2761 tcagtttttg gaaccttgcg ccaagaagga tgtttgcctc caccgttttc cagggaaaca
     2821 gaccatattc ctgtagtacc agaccaattt ttcgcgtagg tccattgatc ggttcaccat
     2881 cgatcgtaat ggatccccgg gaagattgca gtaaccctgc aattatcaga agcagtgtcg
     2941 tttttccgca tccggagggg cccatgatag caagggagtc ccaggatgaa agggtaaagc
     3001 tgacatcttc caaaaccttt tttggtttgt tcaggccggg gaaatactta ctgagtgatt
     3061 ctacgcaaag gaggctattg gacataaccc tcagcgacaa attgcccgta tgtcatatct
     3121 gtttttatga cattcttttc tctaagccag ctgtagacat ccatgacttc tttatttttg
     3181 ggcacgttca atttggggaa tgcaggtatg gcgaagctct gatgaagtgg ttcaggtatt
     3241 ttgcactcgc ggttcatggt aggtcgaacc tcatcagggt tcttgttaat ataatcgttc
     3301 cctttctgta ccgcctgcag aaaggcctta acagcatcag ggttttttgc aaggaactga
     3361 tcactgaaaa caaggaccgt gggagtcaaa ccggtttttc ggtcatcgat cattacccgt
     3421 gctcctttca tctcagccag tgtaaccagg ggttcgggaa ggatagctgc tggaacctga
     3481 tcgctcatca gcatttgcag acgtatagga atgctcttta cctcaacaag gttgatcgta
     3541 ttagcggcaa tccctttttt ttgcagaagc cttgtcatga ggtagtcaag catggtattc
     3601 gagctgcatg caatccctgc agaggaaagg gcatgtaagt ctttttttgg atattttggt
     3661 cctgccagga tggcaaacat cctctgctcg ttctttatgt tgaaatgggt ggcaacaatc
     3721 ttgacgagag ttccgttggc agtcaaaacc attggcgtga tcatatcccc aaaatatccc
     3781 gagatctggc cagcagacat ggcaacgtcc ttttccatgg cagaattgaa gggcaccagc
     3841 tccacgtcga ggcctgcatc cttaaaataa cccttctcag cagccacaaa aagaggcagc
     3901 gcctgtatca caggaagcgt gccaaactgg atggtaacag aaggggattt tcctccttta
     3961 tcctgacttg aaggggaaca gccccaaaat ccatgcattg agaaaacagt cgcaacgagc
     4021 aatatacaaa cctttattga ccacatgaag actcccctgt atataataat attcgtaacc
     4081 cgtaacacgt aatcagtgat cggttttcaa ccccttacgc gttcaccggt cacgggtcac
     4141 ttcctttccc gtcagtcctg ccttttctgc ctatcccgtt tctatcttac gatctctgaa
     4201 acttctcatt caacaccctt ttgaatctcg gagcataaat tatgtcgaag gcatcttctt
     4261 ttccaggaaa aagtttcagg gcatgtttcc tgactccctc gactacccgg atggcttcat
     4321 catgattcag cttttgagat cgcaggtatt caattgcgaa atcaacgatg aatcttaatc
     4381 gccgcagctt tttttcttct tcctgtaaag aatcatccat gttttttttg gggtttagct
     4441 gtaagctgta agcttatagc aaatgctaag tctattttca gttctgccat ctctctcagt
     4501 tatgggctgg tgtatcagat ctctacctat cattgatcac acgtcaccgt ttttcctgtt
     4561 ttcccactct tctggcctta cttcctgtct tgtccttttg tcttttcttc tcccttcaca
     4621 cttttcactt ttacttcctt tcccgccctt ctggccctta tcatttcccg caattcaaca
     4681 aaaagccata aaccgatccc tgtagatatt gcgatatctg caacgttaaa tgctggccag
     4741 tgcaggttct ggtaatgaaa atcaaggaaa tctgtaacgt aaccatagaa tattcgttca
     4801 taaacattcc ccacagcacc agagaggatc aatgtaaggg caagcatctt tcccatagag
     4861 atgcggtata caatcaatat gtaaacaagc gctaagatga tgaggagagg aagataggtg
     4921 aaaaaatgtt ttgcaaaacc aaatccggct aaaataccaa aagccccgcc caaatttctt
     4981 gcatgaacta tggagaaaaa tggagccagc tcgatgattt ccatgtagct caggttttgg
     5041 ataatgagga gctttgtcca tctgtcaagg aggaaaatac aggggattat gaggaggaca
     5101 aggtatctgc gcatcgttta catgtattcg ggaatttccc tgcagcagca gtaatgtctg
     5161 ttgcatactg ccagcatctc tcacatttct gaccatctgc cttggagacg gtaatttctg
     5221 tgtcactgtc ccctttctgc aggtcaatct gagatacaat gaatacatct tttagttcat
     5281 gtcccagccg gccaagcaat gcaaaatcgc tctcacttgc tttaatgaca atccttgtgt
     5341 caagagagtg tccaacaacc ttttcgaccc ttttttcttc aatctttttg ttggccagtt
     5401 ctctgatccg ccagatcttt tcccattctt cctcaacctc aggcctgatc aattccttcc
     5461 tggattccgg aaaagatgcg agaaagatgc tttcttcctg aacataccct ttgagaaaag
     5521 tccacatctc ctcggcagta caggaaagga taggcgcaat aagctttaaa agagaaacca
     5581 gggtctcata tatgactgtc tgggaagcac gcctcttcaa tgcattcttg ttttccacgt
     5641 agatcctgtc ttttactata tcgagataaa gggcactcag gtcaacagtg cagaaattct
     5701 ggactgcgtg atagatggtg tggaaggtgt aggtattgta tgcatctctc actcgttcga
     5761 tgagtctgtg aagtctcgat aaaagccatt tgtcaagata ggaaagttga tcgtaggaaa
     5821 cactgtgctg tgtcggatcg aaatcatcgt ggatgttggc gtggagaaac cttagggtat
     5881 ttctcactct tcggtatgtt tccacgagtc tgttgatgat atctttcgaa attttaatat
     5941 cgtcccggta gtcctcgtaa gttgcccaga gtctcagaat ttcagcgcca tacttctcga
     6001 taatctcctc tggcgctata acattgccta gggatttaga catcttcctt ccggtcccgt
     6061 ctacgacaaa gccatgggtt agaacagact tgtatggcgc ctgtccttcg tttcccaccg
     6121 acgtcagaag agaactatga aaccagcctc tgtgctgatc actgccttcg aggtacatat
     6181 caacggggta ggtaagctcc ttgcgttcct tgcagactgc agcccagctg acccctgaat
     6241 cgaaccatac gtccaggata tcttcttctt tgacaaaggt cgggttgttg cattttttac
     6301 atgtggtccc ccggggcaga aaataggagg catccttctc aaaccagatg tctgcgcctg
     6361 actcctccac tgccttgatc acattcctga aggactcatt actccagaaa ggctctctgc
     6421 acttctcaca atagaatatg gtaatgggga taccccacgt cctttgtcgg gagatgcacc
     6481 agtcaggccg cacctgaagc atgttgaata tcctgtccct cccccacgtt gggatccatc
     6541 tcaccctgtc tatttcatcg agagccttct ggcgcaatcc gtttttgtcg agagatataa
     6601 accattgctc agttgccctg aaaatgactg gttttttgca ccgccagcag tgagggtagg
     6661 aatgctcaat ctcctcctgg tatagaagcc gtccgagttc ctggagtttc ttaatcacat
     6721 gctggttgct atcgaataca ttcatgcccc tgaagaattc gacttcgtca aggaattctc
     6781 ctctttcgtt gaccggggaa tagatatcga ggccgtactc aatccctgtt tcatagtctt
     6841 cttcaccatg ccccggggct gtgtgaacag ctcctgtacc ggtatcagaa gcaacataat
     6901 ctccatatac catcacagaa tccctctcga tgaagggatg tttgaaggag agcttcatta
     6961 atgaatcagc tggtatttcc ccgagaattt tatatcctcc tataccagcc ttttgagtaa
     7021 tttcttcgac aagctctttt aatgcaatgt aaatttctcc gccaacatca aaagcaacat
     7081 acgtgaaggt tgggttgatt gctatggcaa gattggcagg caatgtccaa ggtgtggttg
     7141 tccatatgag cataaagatt ggcttatcag gatacgcggc aaacggtcct tcatagcttc
     7201 cggtataggg gaatttcaca tagatggagg tcgacttctt cagctcatac tcaatctccg
     7261 cctcggccag ggctgtaaga caactcatgc accagtgaac cggttttttc atcctataca
     7321 cctcgtctct tgcaaaaaac ttctgtacct gttctatgat tgtggcctgg tagcggtagt
     7381 ccatggttat gtaaggtttt tcccacagtc ctataccgcc gagcctttta aactcttctc
     7441 tctggacatc aatgaagctc tctgcgtatg ccctgcaccg atgccgaatg tcaagtttcg
     7501 acagagaagc ctttttttcc ttcatttctt tctcgacctg gtgttctatt ggcaatccat
     7561 ggcagtccca tccaggaatg tagtctgcac gaaaacctgt catgaatttg gatttgacaa
     7621 taatatcttt aaggatttta ttcaaagcgg tcccgaggtg gatatgaccg tttgcataag
     7681 gagggccgtc atggagaatg aaaagtggat gattggcagc cctttctacg agtttgccat
     7741 acagcttcat atcttcccat gacttgagca tctccttttc ttttacagga aggttgcctt
     7801 tcatggggaa tggtgtttta ggcagattca atgtgttctt gtagtccatg tgattttacg
     7861 tccttttgaa ttatgtgccc tttcagctat tagctctcag cgaccagttg aagcgacagg
     7921 gcgcacattg agtgggtttt gaaaagaacc ttgtgtgctg tcggctgatt gctgttgccc
     7981 aaagtttctg tgtaaaatta tcacaaagat gataagtatt caagaacaaa ggggaggaag
     8041 agctggaaag gcgggaaagg acgtgaaaag cagtaatgcg tgatctgtaa taagtagtat
     8101 ggcgatgaaa actgtttact gattgtgtgc ttgtataacg gttttttgcc tatgctatac
     8161 ttatgaataa acagataaag aggaggatga tatgacgaca aaacttatgg attatttcaa
     8221 caggatgcca aggttgggat gtctgagcac tgcaagcaaa gaaggtaaag ttaatgtagc
     8281 ctactttggt tctccacgaa tgattgatga caagaccatt gtcatgggtc tgggtaagaa
     8341 caggacattt ttctatctcc aggagaatcc ctacggagtt ttcatgataa tggagccggg
     8401 aaaggtcctt actgagtgga aaggggtaag gctttatgtg aagatgaccg agtgtgccac
     8461 ttcaggttca aagtatgagg agttcaaggc tcagatggca aagagtgcag gagaggcagc
     8521 ggctaaatta attcatgctg ccgttacctt tgaagtctat gaaatgaagc cactagcaga
     8581 ttttggacaa gggtgggaaa agtcaatagg agtatagtaa acccaagacc ttaagctgta
     8641 agctgacagg aaatacagtt tgatcgtttc ttattgatag attgtggggg cagatcgcaa
     8701 cctgccccct tattttttct ataacttaaa gatcaaaaga agcgaaacaa cgagagagta
     8761 tataaccaga gactcaatca tagccagacc gatgatcatc ggggttgtga tctttccgga
     8821 tgcaccggga tttctggcaa tgccgtccaa cgctgacgct atactccgtc cttgtccgat
     8881 agctccaccg aatgctgcga tggcaatacc cagaccagcg gcaagtgcca ccatctgttt
     8941 tacgcctgaa tcgaggccgc ctgttgctgc cccttcagca gccatagcga tactgacgaa
     9001 ggaaaagctg atgaaaactc cgagaagcat ggtaactacg agaacttttt tcataaaatc
     9061 tcaccccttt cttatatttg tttaatgttc ttcatgggaa atagcccctg aaaaatatgc
     9121 catggacaga agcataaaga caaatgtctg gacaaaagca acgaataaac cgagaaacat
     9181 aacaggtaga ggaaccagaa taggaaccag gccgaaaaag atcgcagtca caaggtggtc
     9241 gcctgttatg ttgccaaaga gacgaagacc caaagataaa ggcctcgcta tgtggccgat
     9301 aagctcaaca ggtatcataa gcggggacag tgcccacatt ggtcccacaa actgcttcaa
     9361 atatttgaac ccatgttcct taaatccata atagtgagtc aagacaaaca cggttaggga
     9421 acatgcaaag gttgtgttta cattgtccgt ggcaggaaga aaacccggca cgaggcccga
     9481 gagattgttg aacaggataa aaaggcccag tgttcccacg atgaggacaa actccttgcc
     9541 tcgcggaccc atagtgtcaa caacgatgtt tgcaaggctc tcaactatta gctcaaccgc
     9601 attcctgaat gtcagtttgg gtgaggggac aatatcatcc tcagtctttt tgagttgctt
     9661 gtaacccata ataacgatca cgaggataat tgccgaaacg ataattccat taaatacgtg
     9721 aggctggtct ctcagaattg gaaagagctc ggcccatgac caatcatgcc ccattttttt
     9781 gcctcctttt caaagcaggg ttccaaagac tggaaaactg atttatcact acggaaataa
     9841 ataccgttga taacccaatg aggaaaaaaa ctacatctat atcaccgtat atcacaacaa
     9901 tagctacgag cgcgataagg atgaggaatt ttatcggaag ctttatggca agctctactt
     9961 ttttaccttc gggcttcaac aggccacctt ctataatctt cttgatgaac ctgaaattga
    10021 gggtcataat ggcactagct acagcaaaac taaacacgta tttgaattct ctcatgacaa
    10081 ctatggcaag gatggatcca atcacgagga cgacgatgtt tatacgttca atatcactta
    10141 tctttgtttc tttcattttc cttttcaagc tttttcatca actgatagat agatttcatg
    10201 gctgctacaa taccaaaaat catgaatata atggttaagt aaggagaagt catgaaatat
    10261 cggtcaagga aataaccgat agcaatccca atggccacgc acagacccat ttccaggcca
    10321 agggaactat aacttataag ggatttaacc aaatcccttt tatcatctga taaagacttg
    10381 accatttcga gcccactttt atttatcata cgggcatcgg ttagtcaaac tttttttgtg
    10441 atttctttca ctttttactg agactgatat atctgcctct tattacaggg caatccgttt
    10501 ctaattaatc tgagctgccg gtgattcatg tctttccttc ctgttcaaaa aagaaccccc
    10561 aggcaatccc catcttttta cttcgctcca atagtgccgc atgacttcga ggcatgagtt
    10621 tgttgaatgg cccgttgatt gtcataacgt catagagtta atcaacagat tccgcattca
    10681 atttcttctt gatactgttt tcttctacga gaagacctat ttctttaaaa ggtttttgct
    10741 tgccccaaaa gggtaaacag ggaaggtctc aaagagcatg agatctatcc agctttccct
    10801 gctcgctatg agccctcgag catccgtatc tacgacattg ataacgcggc atccctgaag
    10861 gtcgatgaac tcgcagatga cctttactcc ctttggccat ttccaatcgg cccttcgtct
    10921 tgcaacttct ttttcatctt ccggagacca tgtgcaaata ttcataaagt acataatatc
    10981 agccctcctt ctgatttagt gggcaacatg ccgcccttaa tataagtata gcagaggaaa
    11041 agcctttgtg aaggaacagc gcaaaatttc ttttgaattt tcacagtcct tgtggtataa
    11101 aaaacgctgc atgggccgtt agctcagtcc ggtagagcag ttgactctta atcaattggc
    11161 cccaggttcg aatcctggac ggcccacatg aaaagaagaa attagataag gatggcaggc
    11221 tatatccccc tcaacaatgt gggagagatc atatgaggct ggcatctgtt acaggccggg
    11281 agatagtctg gtaaaaagag tatccccaaa gaatattttc acccattcac atacattgtg
    11341 agagcccatc acctgactac agcatctgtc ttcaggttct ctttttctgc ttcattgcca
    11401 tagaacgttc gttgtaatac agatttccgg ttatattatc ttcaatgtca ataaacggtg
    11461 gtatctgcat attggctgag tctcctgttg tttcaacagg ttctattttc agtatgatga
    11521 ccccctcgta ctttcctgta taagtgtcga gttcacagta ctggttactc cagaggaagc
    11581 agatgcgctc cttttcgatg acatcggttt ttgggtctat gagctttgtg agattgaggt
    11641 attcctgttc tgtgattagc tcttcttctt caaccaatgt tttccgtgaa aggaagtaca
    11701 ggtatgccct gttttgcccg cgcttccgta tccgtatctc ttcattctta ttcttcgagc
    11761 gcagatacat ctgctccatg tggattatct ggtgagtggg caacttttca tggcgaacgt
    11821 tctttacaag gaatttctca gaaatctgaa ggggtgccgg cagtccaaga aaatgagaga
    11881 ttgctgaaaa tgtcctggcg atctttcctt caaagtcagt tgagttgtcg atgattttga
    11941 aatgaggatg gcccagccag ctctcgcgaa ttttctggtc tataatgcgt gcctcctcag
    12001 gcgtttctat ccgtgcgctg ttgttctccc ctgtataaaa tgctggtgca ccttctgctg
    12061 cagtaacaag atggataacc ccattgtacc tatccctgag cctgacctca gtcaacctct
    12121 tttttttcag tatggctcta aaatcgtcat cgggcatgaa tgctttgatg tccatgatcc
    12181 ctctgtcgag gaggatgatt ttttttcgtt ctgggaagat gcgtgttact gtttgctgat
    12241 aggtctcttc aaaggcaagc tgcatatcga gaatagcttc ttcatacatc actacctgtt
    12301 tcgatttgtt catcttacgc ctgtcaatgc cactattggt aatcagggtt gccgtttccg
    12361 ggataacgaa taccataaag ccattatctg acagcctctc tgtaaggtat gccagagagg
    12421 aacttttgcc ggaacaggga cctcccgtaa ggacaatcat ggaaacaggt tcgattaatc
    12481 gagagttctt atacatgcta tacccgggct ttaaatgtga taagcttgtg aggggtaaat
    12541 atatcatccg gtggcatggt aatcaaggga tgtctttccg ggacctttct taaggtgatc
    12601 tatttcgtag atgggtccgg gttggtagca cgggagatgt cactgaaata aatatcctga
    12661 tggggtatct ttttcttcag gatgtcttca ctcttgattt tggaggatct attttgttct
    12721 ggtggtgggc gttttgtcaa tttttttgat actctgtttg taaccaccct gggctgctca
    12781 gtcttcatgg tctccagatc aatcttaaac tggttcactt ctccctttaa tttcacttct
    12841 tccttttcaa ggtcagtgaa tttactttca acatgagaaa tctgtatttt gggatctaag
    12901 gttgcaatct gggtcctgaa ttccctgagg tgagctatgt cattttttag cacagccatg
    12961 tcagatttta gtattattac cattgtccct aatgcgatga ccatgataaa aagggcaacc
    13021 gtccccaagg gaaaggcctt atagtgttgc ctgttttctt cttttggttc ggttatcctc
    13081 ttttttgcct gaaacgtttc cagcaaatcc tcaaaattga ccggggattc cttcttttgt
    13141 ttaacgatct gcatttgtca tgtttcctga gcttatatct tttgacatca aatgaaagaa
    13201 actttgcaaa tatgaggcca ctctattgtg tgctatttct ttaaatgagg taccagcgat
    13261 ttaccatgaa acaggcaatg ggatcaggtt ggtccagtaa aaagtgaaaa ctcaaaggag
    13321 cgaagcaaag gtaaaaaaag cggtgtatgc gtagaccatc agggtatgtc tgaagcactg
    13381 ggaaaaaata gcgtggtacc ttgtctcagg ttgtggaaat cctggatatg ggagttcttg
    13441 gatttgacaa aggcgagaag tcctttgtct gtgcgaccat agaagtcctt tatgagcatt
    13501 ataaaactgt ccccctgccg tactttgact ttaaccatcc tcttcccccg ttctattttg
    13561 gtctgtaaaa gaacggactt gggagcagtc tgaggatcaa cctgggtttt ggaggccatg
    13621 atgtcggttt tagaaatagt gttctttggc ggaaattgag gaacctcctt ctctttcaag
    13681 cgcgctgtgt ttgactgctc tggaacattt tcttgcagag ccttatcctg ttctgattga
    13741 ggatttgtct gtgggacagc gctttgattg gatacagtaa atgatgttga tgtattggct
    13801 gcttgaaggt tgctttcgat cgagacctcc ttttgggaat atataggaga gaaatatccg
    13861 ctgcggaaag cgatcaacag tactacgcac aggcacatga ctgctgccat ggccccggtt
    13921 tgccatctca aatatttttt aaaataagcg tgctgtttgg aacccataag gtcagttatg
    13981 gcttcatttg cagcctcgga tgtgactgat ttcgtttgac gaccaaaagc tgtgacaagg
    14041 gcgttgtcac agagcacatt tataacgcgg ggaattcctt tggcctctct aaggatgcgt
    14101 ttgagggctg attttgtaaa agcagatgta tccctggcgc ctgctttttc aagcctatgc
    14161 tgtacgtatg acaggctttc atctggagag aggggtgtaa tggtggtacg tatggccacc
    14221 cgctgtttaa tgtggcgcaa ttcatttttg tttagcaacg tctcaagttt tggttgcccg
    14281 agtaacacga tttgcagaag ttttttgtct ttggtttcaa ggtttgaaag aacaacaagg
    14341 ttatcgagtg tttcaacggg catgctgtgg gcattatcaa taaaaagtgc gaccgttctg
    14401 tcagccttat cctcatcgag aagaaactgg cgcaattcgg ttatcatttc tgaaatgttg
    14461 tcagttgttg ttaatccaaa ttgtctgcta attgcttgca gaagttcgag aaaggtgatg
    14521 tcagcattgt cgatgtaaat tgttttgaga tattccctgt ccgatttcgc aagaagagcc
    14581 tgggcaacca tggtctttcc cacgccaaca tcaccataaa ttaccatcaa tccttttttc
    14641 ataccaatgc cataaacggt attggccatg gcctgcttat gactttcact taaaaaaagg
    14701 aattcaggat caggggtgat ataaaaaggt tcctttgtca gttgataata atctaaatac
    14761 attgcctttt tcttgcgttt acccccctgt tcctttcaaa tttacagaaa gcaacgatct
    14821 ttttggatcg ttcgatggta cctgaaggat tgcgctccgt ttttccggga ctgaaggagt
    14881 gaaggctacc gacaccgtgc aggttccatt cgtgacgagg gtagaacttg agcaggtatc
    14941 tgcagccaaa gtagagaaat ctccgggatg gtcccctgca atggtgatta cgccgataac
    15001 aagatcagtt atccccttgt tctttagggt cagtattttg ttgacaggga tcccgacgtt
    15061 aaccttgcca aagcttaaag tagatggttt tttgctgatt ttcggaggtt ttgccctggc
    15121 cctgagattt acagtaaata caggtttttt aggatcgtta gaagaaattt tgaggtgcgc
    15181 ttttcgggcg tcataattgt ccgttgatgc gaccactgaa acagtgcaga agtcatttaa
    15241 attagtgagt tccgcaggac aatcatgggc aggagtgagg ctgaagaccg aagcgtcatc
    15301 acctgtaacg cctattgatg ttatttgcaa tggagatacc cctgcatttt tgactgtaac
    15361 tgttagaggt gcagatggta cgctttgttt gaggtttttg aagttgagcg aacgggggct
    15421 gacataaatc ttgtattcag gcaggaagat tgccttgaca gtgatatcat gggtcattgt
    15481 tattgtgcag gtatttcccg aagggtcggg gcaatcggtc cactcaaaga aaacagaccc
    15541 ggaagaaggt gtagcggtaa ctgtgatctc ctcatatgaa ttgtaatagc ctgtgcaagt
    15601 tgctcccaca caggtaaggt ctgttgcagt gatttttcct ttgccggctc cttctttttt
    15661 aacggtcagc acatatggct ttgttcttcc gccccggaca ggccagacaa aagcagtacc
    15721 tgtttttgcc cgagggccta aagcgccatc aaacaaatct atggcccatg ccctgtcagg
    15781 gtcataggca tagctcgtcg aggcccagta tgaatcatct gccccatatt gaacattaat
    15841 gaaatgatgt tttgctggaa gcgctggttc gccttgggat cgatcaatga tgctaaaaag
    15901 ctccttcgcg ttaggcagac gccagtcacc atagccacat gtggtcaggt tattcgtata
    15961 agctaatgca tcgctccatg tttttagtcc atgggcattt gcatttgccg accagaccag
    16021 gccggtcagg ttatctttaa cgatattgtt gatatcctca ccgtcacaat ctgcatcttg
    16081 atcaatacac gggcctgtgg catcacaata tgtcagggta aaacgggggt caggccaggg
    16141 aactcccaca ttgcgaccag gggttgttgc gtaatatgcg tcatcatacg ttgcttttat
    16201 tgagatctgg cctgtctctg caatctgtcc tggaggggca tttttatcag gattgctggt
    16261 tccccgcaca ggccagatta aaccattagc tgtcttaggc tctgcgacaa cagagccgtc
    16321 caccatgtcc atgttccacg cgtttacttc gggactgatt gcgtctgaag tggatgacca
    16381 atattttttg ggcaacacat tcaggaagta aaaaccctgg gacgtgagcc attcaacatt
    16441 attgtattcc tcaaaattaa cgaggctctg gagttcgtta atattgggaa gcctccagtc
    16501 agaaaacccg cattgaaaaa aagcagtcgg cggattcaaa acagacgttg cccaggacgt
    16561 gtaggaagtg aggttccagg cctgtcggga ccacatgagt cctgtaaggt tatccgtgat
    16621 acaggcgcgg ctcttgtcca gttggaagcg cggcataggc caggaaactc cggctatgaa
    16681 atcgccatcc tggcctgtat tatcgcaagg tatgatatca cccgagttac tgtagcaggt
    16741 gctctggccg gtctggggaa gttcgattgt tccg
//
LOCUS       DAOOMC010000060        16761 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdus sp. isolate AMR_MDS_2685
            NODE_3605_length_16761_cov_6.025799, whole genome shotgun sequence.
ACCESSION   DAOOMC010000060 DAOOMC010000000
VERSION     DAOOMC010000060.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864760
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdus sp. (wastewater metagenome)
  ORGANISM  Syntrophorhabdus sp.
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae; Syntrophorhabdus.
REFERENCE   1  (bases 1 to 16761)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16761)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,729
            Genes (coding)                    :: 2,708
            CDSs (with protein)               :: 2,708
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 7 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16761
                     /organism="Syntrophorhabdus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3605_length_16761_cov_6.025799"
                     /isolate="AMR_MDS_2685"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2699756"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            411..830
                     /locus_tag="PK125_07515"
     CDS             411..830
                     /locus_tag="PK125_07515"
                     /inference="COORDINATES: protein motif:HMM:TIGR00149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HPB37992.1"
                     /translation="MKILNNRISVSTKGNEDFLNITQQLNSIFTESGLDNGIVTLFVS
                     GSTAGITTFEYEPGLIQDLKEFYDKIAPANVHYHHDETLGDANGFSHVRAAFTGPSMV
                     IPFEKGRLLLGTWQQVVLLEFDNRPRKRDIIVQLMGE"
     gene            complement(835..2028)
                     /gene="amrB"
                     /locus_tag="PK125_07520"
     CDS             complement(835..2028)
                     /gene="amrB"
                     /locus_tag="PK125_07520"
                     /inference="COORDINATES: protein motif:HMM:TIGR04336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein B"
                     /protein_id="HPB37993.1"
                     /translation="MENPRVRWIDAHPFMDRGNEMILINDVEGIMENSLIVSKDVFFL
                     MSLMDGSRSLRDIQVEYMRIYGELLYMERLEEIVDTMDQNYLLLNENYRLRLTHLKME
                     YEYSSVRKPALAGRSYPANRMELIMVLDEMFKTSPEKKVPGDLTAILVPHIDYTRGLN
                     VYRQIYPYLKHTTKPLIVVFGTCHNMAEKIWNISLKDFETPLDIAPVTQELRSLVEQN
                     NVLREYIAEWPHRKEHSIELQIPLIQFNRLNEFEILPILTGSMHEYIEGIRDIHEDTL
                     TMLIDNLNKVLDEYGKPYIILVGADLAHIGLQFGDSYTLDAYTLTRSKIKDENILSCV
                     KEIDAQAFFDKIKDERDVRKICGLTSIYFLLRLVKGCTAEIISYDQWTDGKSSVSFAG
                     AVFYK"
     gene            2192..3406
                     /locus_tag="PK125_07525"
     CDS             2192..3406
                     /locus_tag="PK125_07525"
                     /inference="COORDINATES: protein motif:HMM:NF018485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPB37994.1"
                     /translation="MKRHYAWVIAFTGTLVTILAHGFGRMSYSVILPSMKEGLALNYT
                     QLGSIATGNFIGYLSLAIIGGFLAARFGVRRVVFVSLVVIGVSLFLTGFSKSFLFAFF
                     MRLISGLGNGGSYVPIMALPAAWFVMRKRGLATGIVSGGIGTGLFISGIILPPIISAF
                     DKDGWRYAWFVLGIAVFILAFVCYAFLRNTPQEKGLTMYGGEEEQRGGPKVTLFSAFR
                     DVVVEPEIWKLGCVYFMYGFSYIIYLTFFMAYLTKEVGVPSIAAGRIFAFLGIISIFC
                     GVIYGWISDVLGRKYGSMIAYINLAISYIIFFLWKDTAGFYVSAFVFGIAAFSIPTIM
                     AAASGDAVGGRLAPAGLGFITLFFGIGQALGPFIGGWLKDVTGTFTHAFALSAAVSLL
                     GAVGSLILRKKV"
     gene            3525..4220
                     /gene="radC"
                     /locus_tag="PK125_07530"
     CDS             3525..4220
                     /gene="radC"
                     /locus_tag="PK125_07530"
                     /inference="COORDINATES: protein motif:HMM:TIGR00608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HPB37995.1"
                     /translation="MMKEGTTFTVHDMPKQERPRERLQRLGPDALSSHELLALIIGRG
                     IPKRSVLDIAHDLLRKFGSVQGISKATMEELSAISGIGTAKAAQIKAAFELAKRQDLE
                     QDIPPYTVNNPHILVKAIRATIQDKAKEHFKLVLLNTRNKVTGIIPISVGTLNASLVH
                     PREVFKEAIHGNAASVILVHNHPSNDLEPSEEDVRLTRRMVEVGTIMGIEVLDHIIIS
                     KNDFLSMKARGLW"
     gene            4388..6142
                     /gene="aspS"
                     /locus_tag="PK125_07535"
     CDS             4388..6142
                     /gene="aspS"
                     /locus_tag="PK125_07535"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: protein motif:HMM:NF001750.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HPB37996.1"
                     /translation="MRDRYCGKVSIDDVGKTLTLSGWISGRRDLGRLIFIDLRDVTGI
                     FQVVFSPDISPEAHERAGDLKQEYVIQVSGKVQNRPDGTVNASMPTGALEMYVVSFQV
                     LNTCKPLPFQLDEENVSELMRLKYRYLDLRRPLVQKIFLERSTAYKVTRDYLASHGFY
                     EFETPFLTKSTPEGARDFIVPSRLNAGTFYALPQSPQLFKQILMVSGFDRYFQIVRCF
                     RDEDLRADRQPEFTQVDMEMSFVEVDDVIGMCEGLVASIYEAIRGEPLPLPIPRMTYE
                     EAMEVYGSDKPDLRFELPMTDLSDIFRETEFGVFRKTIDDGGAIKSIVLKGKTLSRKD
                     LETAVGITKEMGAGGLIWIRKEKEGLQSTIVKYLKDYEKSEMSERLHLSDGDVAFIMA
                     DAKNKVNDVMGRFRLYLGDKFEPMAKDQFKFLWIVDFPLFEYSNEDKRFVARHHPFTS
                     PQGSIKDFNGDYESFKAKAYDLVLNGVEIGGGSIRNHRMDDQLQMFKLLNISQDDAIE
                     KFGFLLEALEMGAPPHGGLAFGFDRILMMLLGLESIRDVIPFPKTQKGTCLLTGAPSK
                     IPQKQLNELRIRLTGEQQ"
     gene            6217..8025
                     /locus_tag="PK125_07540"
     CDS             6217..8025
                     /locus_tag="PK125_07540"
                     /inference="COORDINATES: protein motif:HMM:NF012885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB37997.1"
                     /translation="MSKKTGFIFKNVEKGARYSLIDVEEPELYRGMFPYSEVCKIKFD
                     NKIELIDPPEEIYITDTTFRDGQQSRPPFTPKQIEDLFEFLHRLGGPNGVIKQSEFFL
                     YTEKDRQTVEKCLEKGYRYPEITGWIRANKDDLQLVKELGLKETGILTSVSDYHIFLK
                     LKKTRKQVFKDYIQNIEEALEHNIVPRCHFEDATRADVYGFCVPFAQTLMQLSEDAKI
                     PVKIRLCDTLGIGITYPGAALPRSIGKLIRAMIDDAGVPPEYLEWHGHNDFHKVVINT
                     VSAWLYGCSHANGTLLGIGERTGNAPIEGLIMEYISLMGNENGIDTRVITEIRNYFEK
                     EIGFQIPRNQPFVGVDFNATSAGVHIDGILKNEEIYTPFDTSRILNRPLAINITDKSG
                     LAGITHWINSHFALIGKERLEKTHPAIGRINKWIIKQYDEGRITCISDNEMEHLVRRY
                     IPEIFVSEFERLKNKAYDMAAHLITRYIEDSAIKSMNPDKQQETLKNIVREYPYIQFA
                     YSVNSEGIKITKNITQAVDRAKYYKIGLHEDFSDRDWFLNPMKTGEISVTDLYTSKIT
                     GALCVTVSGPIRDEFGDIVGVLGLDIKFEDLTKAEE"
     gene            8029..9324
                     /locus_tag="PK125_07545"
     CDS             8029..9324
                     /locus_tag="PK125_07545"
                     /inference="COORDINATES: protein motif:HMM:TIGR02086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HPB37998.1"
                     /translation="MGKTIAEKILSMNTKTDLKAGDYAVVKVDRILLQDGTAPLAINR
                     FKEMGFTRLFDGDRITFFIDHASPSSRMELSNDHQQIRDFAKSNNARISEVGEGICHQ
                     VIAEHVLCPGEVLVGADSHTCTGGGLGAFATGMGSTDIAVAMGLGQTWLRVPEAIRFN
                     INGKLRSGVFPKDIILAIIGMMGADGATYKSMEFTGETVKHLNIEDRLTISNMAVEAG
                     AKCGLFSSDLMTFLYLKRLKREKSYIEIQPDDDANYEKVVEINAELLTPMVSFPDQVD
                     NVKSIDDPDVKGVNIHQVFIGSCTNGRVDDLRIASIILKGRKIQKGIRLLVGPASRMV
                     YHQALQEGIIATLFQAGAVILPPGCGPCIGIHQGVLGDGETCLSTSNRNFLGRMGNPN
                     SHIILASPATAAATALKGKLTDPREVLDDFERKGVEVWR"
     gene            9287..9784
                     /locus_tag="PK125_07550"
     CDS             9287..9784
                     /locus_tag="PK125_07550"
                     /inference="COORDINATES: protein motif:HMM:TIGR02087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HPB37999.1"
                     /translation="MILKGKVWKFGDNISTDHIIPGRFYHLRSNLEELKKHVFEDISP
                     GFCKKVKKGDIIVGGKNFGLGSSREHAPLIIKMAGIDAIIATSFARIFYRNAINVGLA
                     AIICNTDGINEGDSLEIRVDDGMLVNKTTGDEKSFSPLSSIMRTILNEGGLDHYIARY
                     GGFKI"
     gene            9781..10932
                     /gene="icd"
                     /locus_tag="PK125_07555"
     CDS             9781..10932
                     /gene="icd"
                     /locus_tag="PK125_07555"
                     /inference="COORDINATES: protein motif:HMM:NF005425.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HPB38000.1"
                     /translation="MTDIVTIPYIEGDGAGDDIWHASKEVLEESVQKAYGGKKRIKWI
                     SLLAGEKALAKTGELLPEETLQTVKAHQVAIKGPLTTPVGGGFRSVNVYLRQIFDLYV
                     CMRPIRFLPGLPSPLKKPEHVNFIIFRENTEDLYKGIEWRAESKESLIFLTFLREKMG
                     VSLTDDTGVGVKPISRTGTARFTRWVLDYAIKNNRKAVTVVHKGNIMKYTEGAFREWA
                     YEVARGYADKLTFSGEKDKILFNDRIADNMFLQVILRPEDYDILLCPNLNGDYLSDAC
                     AALIGGLGVAPGANFGDNVAIFEPTHGSAPKYAGKDLSNPTSFLLCGAMLFRHINLEK
                     AADILEYAIEETVGNKIVTYDLARQMDNVRPVRCSEYGEVIKKRIEQRA"
     gene            10993..12109
                     /gene="prfB"
                     /locus_tag="PK125_07560"
     CDS             join(10993..11061,11063..12109)
                     /gene="prfB"
                     /locus_tag="PK125_07560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_156919602.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HPB38001.1"
                     /translation="MIEDINTRIDAIVERMAILRGYLDLDNLNRQINALDEELSKQAL
                     WDDQDKAQTKLKQRSRLQSEIENWNHKEKDLEEILILGEFVHETDDQKDLEELVERVN
                     SLDQALDRYEIERMLSEENDDSNAIVYINAGAGGTEAQDWAEMLLRMYLRWAERKGFE
                     TQVVDILPGEEAGIKNVTFLVKGPHAYGYLKCENGIHRLVRVSPFDANNRRHTSFASF
                     YAYPELADNIDVEIRDEDLKVDTFRSSGPGGQHVNKTESAIRITHIPTGIVVQCQNER
                     SQHKNKAIAMAILRSRIYEIEKKKQEEKMNSLEKEKKDIAWGSQIRSYILHPYKLIKD
                     HRTKTEIHNADSVLDGEIDPFIKAYLLYLTFEQKSDT"
     gene            12117..12350
                     /locus_tag="PK125_07565"
     CDS             12117..12350
                     /locus_tag="PK125_07565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB38002.1"
                     /translation="MELLSENRIIELEEKIDNLIKNYKGMKEEHEKLLGRVKSLETEN
                     TDLKTKMADVKNERELLIEKVTKILEKVDKVEA"
     gene            12620..12797
                     /gene="ssrS"
                     /locus_tag="PK125_07570"
     ncRNA           12620..12797
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="PK125_07570"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            12957..14513
                     /gene="rny"
                     /locus_tag="PK125_07575"
     CDS             12957..14513
                     /gene="rny"
                     /locus_tag="PK125_07575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HPB38003.1"
                     /translation="MNINIIIIIVSVVIALCFGFVLARIIQRKKIGEYEKIGKKILED
                     AKKEADVLTREASIQAKDVALQAKNELEQEIKSRRLELQNSERRLVQKESSLDKKTEQ
                     IEKKEEELAKREKEVSSRQSYFDTRIKEQDALIKEGKARLEKISGMTAEEAKKTLMQA
                     MENEAKYEALKICKKIEEEAREKADREAKKIIALAIQRYAGDYVGEDTVSTVPLPNEE
                     MKGRIIGREGRNIRALEAATGVDIIIDDTPEAVILSCFNPIRREVAKIAISRLISDGR
                     IHPARIEEIVSKVAEEMEDRIKEAGEQAVFDLGVHNVHPEIVKLLGRLTFRSSYAQNV
                     YQHSLEVAFICGVIASELRVNVKEAKRAGLLHDIGKAVDHEIEGSHASIGADLARKYG
                     ENETIVHAIMSHHEDVEPTGILDVIVQAADALSGARPGARREMLESYIKRLEELERIA
                     NNFPGVDKSYAIQAGREIRIVVNSEKINDDNIYLLSRDIAKKIESDLSYPGQIKIVVI
                     RETRAIEYAK"
     gene            14503..15279
                     /locus_tag="PK125_07580"
     CDS             14503..15279
                     /locus_tag="PK125_07580"
                     /inference="COORDINATES: protein motif:HMM:TIGR00282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HPB38004.1"
                     /translation="MPNEIKVLFLGDVIGKPGRKAVEEFVKSFKADFVVINGENLAGG
                     IGITPHVAQEMFSCGVDIITTGNHVWKKKEMIPFLMEEQRVLRPLNYPSGTPGFGHSI
                     TRKNGKRICIVNLEGRVFMTPLDCPFRAIGNLLKELDDKIPIIIDFHAEATSEKIALG
                     WYLDGKVAAVVGTHTHVQTADERILPKGTGYITDAGMTGPCDSVIGMEKSVVLKKFVT
                     QLPQRFEVGKNDIEVQGVVLTLENEGNSCIAIERIKQKIV"
     gene            15378..16601
                     /gene="tyrS"
                     /locus_tag="PK125_07585"
     CDS             15378..16601
                     /gene="tyrS"
                     /locus_tag="PK125_07585"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HPB38005.1"
                     /translation="MFDVDKQMEVIKRGAVDLVNEEELRSKLERSIRENKPLRVKLGL
                     DPTAPDLHLGHTVVLQKLKQFQELGHVAIFLIGDFTGMIGDPTGRIETRPALTKDELL
                     VNAETYKKQVFKILDPETTEVRFNSEWFETMNAADMIRLCAQYTMARMMEREDFRNRY
                     QNNLPISIHEFLYPLVQGYDSVALQADIELGGHDQIFNLFVGRVIQRAYGQESQVLVT
                     VPLLEGTDGINKMSKSYGNYVGIDEQPDTMFGKLMSISDELMLKYYELLSDISLDKLR
                     ILKEEIHNGSVHPRDAKVNFAKEIITRFHSKEAADAAHENFDKMFRDKEVPEDIEVVT
                     IKKSETGTWLPKLLATIGMVTSTSEGKRMVQQGGVHINGVKVNSEEITLDAVNELTIK
                     VGKRKFKKVIFENES"
BASE COUNT         5164 a         3118 c         4006 g         4473 t
ORIGIN      
        1 cttcgtgttt ttctccgctt tccttcggct cacgtctttt tgttactttt ctcagggctt
       61 gcgtctgtct gcgccagact ttgatagata gattcacccc ttcttcaaca gtacgtgtaa
      121 cctattgatt ggctgagtat attgagagtg cctttggaat tttttgcttt tatgtatatt
      181 tcgggaagtg gttaaatagg tgattgtaag gcagaacccc tggttttttg atgtgacgac
      241 atagaaatgt gatagcaggg tgaggggggt tcaacaagga gaagctgaaa aataaatatc
      301 aggcccaagt gactaagctg gtggacaact ttgaaaaatt ataggatatt acagaacagg
      361 ctgccttcta atctaaaaca acagattatt tgtgaaaagg agattatgtg atgaagattt
      421 tgaacaatag aattagcgtt agcacaaagg ggaatgaaga tttcttgaac ataacgcagc
      481 agttgaacag tattttcact gaatctggcc tcgacaatgg aattgtgaca ctttttgtat
      541 caggttcaac agcaggcatt acaacatttg agtatgaacc ggggctgata caggatttaa
      601 aagagtttta tgataaaata gctcccgcaa acgttcatta tcatcatgat gaaactttgg
      661 gtgacgcaaa cggatttagc cacgtcaggg cagcttttac aggcccctca atggtaatac
      721 catttgaaaa gggtcgactg ctattgggca catggcaaca ggttgttctt ttagagtttg
      781 acaacaggcc acgtaagcgt gacataatag ttcaattgat gggagaatga atttttattt
      841 ataaaaaact gctccggcaa agcttactga tgatttgcca tcagtccatt gatcgtagct
      901 aataatctca gcagtgcatc ctttgaccaa ccttaaaagg aaatagatgg acgttaaacc
      961 acatattttc cgcacatctc gctcatcttt gattttatca aagaaggctt gtgcatcaat
     1021 ttctttcaca caagacagta tgttctcatc tttgatcttc gagcgcgtta gtgtatatgc
     1081 atccagtgtg tatgaatcgc caaattgaag gccgatatgt gcaaggtctg cgcctaccag
     1141 gataatatat ggctttccgt actcgtctaa aactttattg aggttatcta taagcattgt
     1201 aagcgtatct tcatgaatat ccctgatgcc ttcaatgtat tcgtgcattg aaccggtgag
     1261 tatagggagg atttcaaact cattcagcct gttaaactga atgaggggaa tctgaagctc
     1321 aatggagtgt tcttttctgt gtggccattc ggcgatatac tcgcgaagca cattattttg
     1381 ttcaaccaag ctcctgagtt cctgtgtgac aggggcaata tccaggggtg tttcgaagtc
     1441 cttgagagaa atattccaga ttttttcagc catattatga catgtgccaa aaacaacgat
     1501 aagaggcttg gttgtatgtt tcagatacgg ataaatttgt ctgtatacat tgaggccccg
     1561 cgtatagtca atatgaggaa caagtatagc agtcaagtcg ccaggaactt ttttctctgg
     1621 tgaagttttg aacatttcat caagtaccat gatgagttcc atcctgtttg cagggtagct
     1681 cctgcctgca agagcgggtt ttctcacaga cgaatactca tattccattt tcaaatgtgt
     1741 aaggcgaagc ctgtagtttt cattgagaag taggtagttc tggtccatgg tatcaacaat
     1801 ttcttcgagg cgttccatgt atagaagttc cccatagatt ctcatatatt cgacctgaat
     1861 gtcacgcagg gagcgagaac catccataag tgacataaga aaaaaaacat ctttcgaaac
     1921 tatgagagaa ttttccatga tcccttcaac atcatttatc agaatcatct cgtttcctct
     1981 gtccatgaac ggatgtgcgt cgatccaccg cactctcgga ttttccatgt cattttatac
     2041 cataaggtac ggcccaaaaa aaaggtttat cagtgattga aactggcaat aggcgcatcc
     2101 gagatgagaa atcgaggaaa caaaaacggt tgacatctat gtgtgtgctt tatatgcttt
     2161 atggaatgac ataaaaaata aggggggggg tatgaaaaga cattatgcat gggttatagc
     2221 attcacgggc actctcgtaa caatcctggc ccatggtttc ggacgtatgt cttattctgt
     2281 gattcttcct tcaatgaagg aggggttggc actcaattat actcaacttg gttctattgc
     2341 cacaggaaat tttattggat atctttctct tgctataatc ggaggttttc ttgctgcgcg
     2401 ttttggagtg agacgggttg tttttgtttc tctcgtggtg attggggtca gccttttcct
     2461 tacagggttt tctaaatcct tcctttttgc gttttttatg aggcttatat ccggtcttgg
     2521 caatggagga agctatgtcc caatcatggc attgcctgca gcatggtttg taatgagaaa
     2581 acggggactg gcgacaggta tcgtatctgg cggcatagga acaggacttt ttatatctgg
     2641 catcattctt cctccaatta tttctgcctt tgataaagac ggttggagat acgcctggtt
     2701 tgttctgggc atagcggtat ttattctcgc ctttgtatgt tacgcatttc tgaggaatac
     2761 tccacaagaa aaaggtctta ccatgtatgg tggggaggaa gagcagaggg gaggacctaa
     2821 agtcactctt ttttcggcat tcagggacgt tgttgttgaa cctgaaatat ggaaactggg
     2881 atgcgtctat ttcatgtatg gtttttcgta cattatttat ctaacctttt ttatggcata
     2941 cctcacaaag gaagtgggag ttccctcaat agctgcagga agaatatttg cattcctcgg
     3001 aattattagc attttttgtg gtgtcatcta tggatggatt tctgatgtgc ttggcaggaa
     3061 gtatggctcc atgatcgcat atattaatct tgcaatatca tacataattt tttttctgtg
     3121 gaaagatacc gctgggttct atgtttctgc ctttgttttt ggtatagctg ccttttcaat
     3181 accaactata atggctgctg cgtcaggtga tgctgttggt ggtcgtcttg caccagctgg
     3241 ccttggcttt attacccttt tctttgggat tggtcaggct ctgggtcctt ttataggggg
     3301 atggctcaaa gatgtgacag gcacatttac ccatgcattt gcactgtcgg cagcggtttc
     3361 cttgttaggt gcagttgggt cattaatatt gagaaaaaaa gtttagccat tggcagccca
     3421 ggcatggtta ggagggtgca gtgcctcctg cattgatgat ttcagttata aaattgcttt
     3481 gcaaacctgg taggagttcc aatgaaactt gatgaggctc tatcatgatg aaagaaggaa
     3541 caacgtttac cgtccacgac atgcctaaac aggaacgccc cagggagcgc ttgcagaggt
     3601 tgggccctga tgcactttct tcacacgaac tcctagctct tataataggc aggggtatac
     3661 caaaaagatc agttcttgat attgcacatg accttttgcg aaagtttggc agcgtacaag
     3721 ggatcagcaa ggctactatg gaggaactat cagcgataag cgggattgga acagcaaaag
     3781 cagctcagat caaagctgct tttgagcttg caaaacgaca ggatttggag caggatattc
     3841 ctccatacac ggtcaataac ccccacatat tggtcaaggc aattagggca accattcaag
     3901 acaaagcaaa ggaacacttt aaactggttc ttctcaatac gcgaaacaag gtaacaggga
     3961 ttattccaat ctctgtaggt acattaaatg caagcctggt ccatccaaga gaggttttca
     4021 aagaagcaat ccatggaaac gcagcatctg tcatccttgt acacaaccat ccttcaaatg
     4081 atctggaacc atccgaagaa gatgtaaggc ttactcgacg catggttgag gtagggacca
     4141 tcatgggcat cgaggtgctg gatcacatca taatcagcaa aaatgatttt ttaagtatga
     4201 aagcaagagg tttgtggtag gttttgaaca ataaacccct gctttatggc gttaagtttc
     4261 tatccactaa tttctgaact gaaacgaagc cgtttcttat tcactgaagc atgtgattgg
     4321 ccaaggcaaa tcttgactcg tgcagctaat accatatata ataatagact tttttaggag
     4381 ctaacccttg agagatagat attgcggcaa agtttcaata gatgacgttg gaaaaacact
     4441 gaccttatcg ggctggatct ctggaagaag agatctgggc aggctgattt ttatcgattt
     4501 acgggatgtg acaggaattt ttcaggtcgt tttttcacct gatatttctc cggaagctca
     4561 tgagagggcg ggtgatttaa aacaggaata cgtaattcag gtgagtggta aagtgcagaa
     4621 tcgccctgat gggacggtaa atgccagcat gcctacaggt gcccttgaaa tgtatgtcgt
     4681 ttcttttcaa gtactgaaca catgtaagcc attgccattt cagcttgatg aagagaatgt
     4741 cagcgaatta atgcgactaa aataccggta ccttgacctg cgtcgacctc ttgtgcagaa
     4801 gatttttctt gagcgtagca cggcctacaa agtgaccaga gactatctgg catcccatgg
     4861 cttctacgaa tttgagactc ctttcctgac caaaagtact ccagaagggg cgcgtgattt
     4921 tattgttccc agtaggctga atgcagggac tttttatgcg ctgcctcaat ccccccagct
     4981 ctttaagcag atacttatgg tgagtggttt cgatcgttat tttcagattg tgcgatgctt
     5041 cagggatgag gacctgcggg cagacagaca gccagagttt acgcaggtcg atatggaaat
     5101 gagttttgtt gaagttgatg atgtcatagg catgtgtgaa ggccttgtgg cttcaatata
     5161 tgaagcaata cggggagaac cactcccatt gcccatcccg agaatgacat atgaagaagc
     5221 aatggaagtt tatggaagcg acaaaccgga tcttcgtttt gaactaccca tgacagatct
     5281 gtcagatatt tttcgcgaga ctgaatttgg tgtcttcagg aagactatag atgatggcgg
     5341 tgccatcaag agtattgtat taaaagggaa gacactttca agaaaggacc tcgaaacagc
     5401 tgtggggata accaaagaga tgggtgctgg tggactcatc tggatacgga aagaaaaaga
     5461 aggattacag tcaacaatcg taaaatacct aaaagactat gaaaagtctg agatgagtga
     5521 gaggttgcac ctttcagatg gagatgttgc attcattatg gctgatgcta aaaacaaagt
     5581 aaatgacgtt atgggaaggt ttagactcta cctgggagac aaatttgaac ccatggcaaa
     5641 agaccagttc aaattcctgt ggattgttga ttttccgctt tttgagtaca gcaatgaaga
     5701 taaacggttt gtagcacggc accatccatt cacctccccc cagggtagca ttaaagattt
     5761 taacggtgac tacgaatcat ttaaggcaaa agcctacgac cttgttttaa acggtgttga
     5821 aataggtggc ggcagtatta ggaaccaccg tatggatgat caactgcaga tgtttaagct
     5881 tctcaatatc agtcaagatg atgcaattga aaaatttgga ttccttctcg aagcccttga
     5941 aatgggtgct cccccacatg gtggcctggc ctttggtttt gacaggattc ttatgatgtt
     6001 gcttggcctg gagagtatac gtgatgttat accttttcca aaaacacaga agggcacctg
     6061 tcttttaacc ggggcaccat cgaaaatacc acaaaaacag ttgaatgaat tgaggattcg
     6121 tcttacaggg gaacagcaat aaggaaatgg aaccgccagg cattaggatt ttccataatc
     6181 attgtgctgt tctcactgca caaacaggag gaaatagtga gtaaaaagac aggttttata
     6241 ttcaagaacg tggaaaaggg ggccaggtat tccctcatcg atgtggaaga gccagagttg
     6301 tatagaggga tgttcccata ctctgaagtc tgtaagatta agtttgacaa taagatagag
     6361 ttgatcgacc ctcctgaaga aatctacatc acggatacaa cattcaggga tggacagcag
     6421 tcgaggccgc ccttcacacc gaaacagatt gaagatctct tcgaatttct tcatagactc
     6481 ggcggcccaa atggtgtcat aaaacaatct gagttttttc tctatacaga aaaggacagg
     6541 caaaccgttg aaaaatgcct tgaaaaaggc tacaggtacc ctgaaatcac aggatggata
     6601 cgcgcgaaca aagatgattt acagcttgtt aaagagcttg gcttaaagga aacgggcatt
     6661 ctgacgagcg tttcagacta tcatattttt ttgaaactta agaaaacacg taaacaagtg
     6721 ttcaaagact atattcaaaa tattgaagaa gctctcgaac ataacattgt tccgagatgt
     6781 cattttgagg atgcaacacg ggctgatgtg tacggattct gcgtaccctt tgctcaaaca
     6841 cttatgcaac tctcagaaga tgcaaaaata ccggtgaaaa taagactctg tgacacactg
     6901 ggaattggaa ttacctatcc gggcgctgct ttaccaaggt ctatcggaaa gctgattcgg
     6961 gcaatgatag acgatgctgg ggttccgccg gagtaccttg aatggcatgg acacaatgat
     7021 tttcacaaag tggtgatcaa cacagtaagt gcctggcttt atggctgttc ccatgccaat
     7081 ggaacactcc tgggtattgg ggagaggaca gggaatgccc ctatagaagg cctgattatg
     7141 gagtacatat ccctcatggg gaacgaaaat ggcatcgata ccagagtaat taccgaaatc
     7201 agaaactact tcgaaaaaga gataggcttt cagattcctc gaaaccagcc ctttgttggc
     7261 gtagatttta atgccacatc agcaggtgtg cacatagatg gaatcctgaa aaacgaagag
     7321 atctacaccc cctttgacac tagtcggatc cttaacagac ccctcgcgat aaatattact
     7381 gacaaatcag gtctggcagg aataacacac tggataaact cacattttgc acttattggc
     7441 aaggaacggc ttgagaaaac acatcctgct atcggaagga ttaacaagtg gattataaaa
     7501 cagtacgacg agggtaggat tacttgtata tctgacaacg agatggaaca ccttgtgcga
     7561 agatacatac cggaaatttt tgtatctgag ttcgagcgtc tcaaaaataa ggcctacgat
     7621 atggctgccc acctgatcac aaggtacatc gaggatagcg cgatcaagag catgaatcct
     7681 gataagcagc aagagacctt aaaaaatatc gtccgggaat atccttacat tcagttcgcc
     7741 tactcggtga acagcgaagg tattaagatt acaaagaata ttacgcaagc ggtcgacaga
     7801 gcaaaatact acaaaattgg cctccacgag gatttctcag atagagattg gtttctcaat
     7861 cccatgaaga ctggtgaaat tagcgtgact gatctttata cttcaaagat tacaggggcg
     7921 ctctgtgtta ccgtgtccgg cccgataagg gatgaattcg gtgatattgt tggagtgttg
     7981 ggtctcgata ttaagtttga agatcttact aaggctgagg aataattcat gggaaagact
     8041 attgcggaaa aaatcttgag tatgaacaca aaaacagacc tcaaggcagg ggactatgct
     8101 gttgttaaag tagaccgcat attactccaa gacggcacag ccccattggc aataaacagg
     8161 tttaaggaaa tgggtttcac ccgattgttc gatggagacc gcatcacctt ttttattgac
     8221 catgcatcac caagttcgcg aatggaactc tcgaatgatc atcagcagat tcgggatttt
     8281 gcaaagtcaa ataacgcgcg tatatcggag gtcggggagg gaatctgcca ccaggttatc
     8341 gcggaacatg tactctgccc aggcgaagtg cttgtaggtg ctgattcaca cacgtgcaca
     8401 ggtggtggcc tgggtgcatt tgcaacagga atgggttcga cagacattgc ggtggccatg
     8461 ggcctcgggc agacatggtt aagagtccca gaagcaataa ggtttaacat taacgggaaa
     8521 cttcgctcag gtgtttttcc gaaggacatt atccttgcaa ttattggaat gatgggtgcg
     8581 gatggagcaa cctacaaatc aatggaattt actggagaga cagtcaaaca tctcaatatt
     8641 gaagacagac tcactatatc aaacatggct gttgaggcag gagctaagtg tggattattt
     8701 tcatctgatc taatgacatt tctttatttg aagagattga aaagggaaaa atcatatatt
     8761 gagatccagc ccgatgatga tgccaactat gagaaggtag ttgaaattaa tgctgaactc
     8821 ctgactccga tggtcagttt cccggaccag gtagataacg taaaaagcat tgatgatcct
     8881 gatgttaaag gtgttaacat acatcaggtc tttatcggat cgtgtacaaa cggaagggtt
     8941 gatgatctcc gcattgcttc tattatcttg aaaggacgaa agattcaaaa agggattcga
     9001 ctccttgtag gcccagcgtc gaggatggtc taccatcagg ctcttcagga aggcattatt
     9061 gctactcttt tccaggcagg agcagtgatt cttccaccag ggtgcgggcc ctgtatcggt
     9121 attcatcagg gggttcttgg ggacggggag acctgccttt caacctctaa tagaaatttt
     9181 ctcggccgga tgggaaaccc caattcacat attatacttg cctcaccggc aacagcagct
     9241 gcaaccgcgc ttaaaggaaa attaacagat ccacgggagg ttcttgatga ttttgaaagg
     9301 aaaggtgtgg aagtttggag ataacatctc tactgaccat atcatcccag gaaggttcta
     9361 ccacttgcgc tcaaaccttg aagaactaaa aaaacatgtt tttgaagata tctcacctgg
     9421 tttttgtaaa aaggttaaga agggcgacat tatagtaggc gggaaaaact tcgggcttgg
     9481 ctccagcaga gaacatgcac cacttattat aaagatggca gggattgatg caattatcgc
     9541 aacatccttt gcacgaatat tctataggaa tgcaataaat gtgggtcttg cagcaataat
     9601 atgcaataca gatggaatta atgaaggaga ttcccttgaa ataagagtcg atgacggtat
     9661 gctggtaaat aaaacaacag gcgatgagaa gagtttttca cccctgagtt cgattatgcg
     9721 tacaatcctg aatgagggag gacttgatca ctatattgca cgttatggag ggtttaagat
     9781 ttgactgata ttgttacgat tccctatata gaaggtgatg gtgcaggtga tgatatctgg
     9841 catgcttcaa aagaggtcct tgaagaatcg gtccagaaag catacggagg taaaaaaaga
     9901 ataaaatgga tatctttgct ggcaggagaa aaggctctag caaaaacagg tgaacttctc
     9961 cccgaagaaa ccttgcagac tgttaaggcg caccaggtgg caattaaagg gcccctaaca
    10021 acacctgttg gaggaggttt tagaagcgta aatgtatatc tacgccaaat atttgatctt
    10081 tatgtttgta tgcgtcccat cagatttttg ccagggcttc cgagtccgct gaaaaagccc
    10141 gaacatgtta attttattat tttcagagaa aacacagaag acctttacaa agggatagaa
    10201 tggagggctg agagtaaaga gtcattgata ttccttacat ttctcagaga aaaaatgggg
    10261 gttagcctaa ctgatgatac cggtgtaggc gtcaaaccga taagcaggac aggtactgca
    10321 cgatttacca ggtgggttct tgactacgcc atcaagaata acaggaaggc agtgacagtt
    10381 gttcataagg gcaatatcat gaaatacaca gaaggcgcct ttagagaatg ggcatatgag
    10441 gtagcaaggg gctacgctga caagttaact ttttctggtg aaaaggataa aattcttttc
    10501 aatgaccgaa tagccgacaa tatgttttta caagttattt taaggcccga agattatgat
    10561 attcttctgt gcccgaatct taatggtgat tatctgtctg atgcttgtgc agcccttata
    10621 ggaggtctgg gagttgcgcc tggagctaac tttggtgata atgtggcaat ttttgagccg
    10681 acccatggga gtgctccaaa atatgcagga aaggaccttt caaatccaac gtcattcctt
    10741 ctatgcggag cgatgctctt cagacatatc aaccttgaaa aggcagcaga tattcttgaa
    10801 tatgcaatag aggaaacggt gggtaacaag attgtcacct atgatcttgc acgacaaatg
    10861 gataatgttc gtcctgttag atgttcggaa tacggggagg taataaaaaa aagaatagag
    10921 cagagggcat agcactgcac gttagcgagt gtttgtttgc agactgctta gagtgagaaa
    10981 tgaggtaata ttatgataga agatatcaat acaagaattg atgcaattgt cgaaagaatg
    11041 gccattctca gaggctatct ttgacctcga taacttaaac cgtcagatta atgcacttga
    11101 cgaagaactt tccaaacagg cactctggga cgaccaagac aaagcacaaa caaagctaaa
    11161 acagcggtca agacttcaat cagaaattga gaattggaat cataaagaaa aggatcttga
    11221 agaaatcctt attcttgggg aatttgtaca cgaaactgat gatcagaaag atcttgaaga
    11281 actagtagaa agggtaaatt ctcttgatca ggctcttgac cgttacgaga ttgaaagaat
    11341 gctcagcgaa gagaacgatg attccaatgc cattgtttat attaatgccg gggcaggtgg
    11401 aacagaggct caggattggg ctgaaatgct tcttaggatg tacttacgct gggctgaaag
    11461 aaaagggttt gaaactcagg tagtggacat acttcctggt gaagaagcgg gaattaagaa
    11521 tgttaccttt cttgtaaagg ggccccatgc ttatggttat ctgaaatgcg agaatggaat
    11581 ccacagactt gttagggtat ccccgtttga tgcaaataac aggaggcata cctcttttgc
    11641 gtcattctat gcataccctg aacttgcaga caatattgat gtggagattc gggatgaaga
    11701 tttaaaagtt gatacctttc gttccagcgg tcccggggga cagcatgtaa ataaaacaga
    11761 atccgctata aggattaccc atattccaac aggtatcgta gtccaatgtc agaatgaaag
    11821 atcacagcat aaaaacaaag ctatcgccat ggcaattttg cgatctagaa tctatgagat
    11881 cgaaaagaag aaacaagagg agaagatgaa ctcactggag aaagagaaaa aggatattgc
    11941 ctggggaagc cagatacgtt cttatatact ccatccatac aagcttatca aggatcaccg
    12001 gacaaaaaca gagattcata acgcagattc agtgcttgat ggtgagattg atccctttat
    12061 caaggcgtac cttttgtact tgacttttga acagaaaagc gatacttaaa taggttatgg
    12121 aactccttag tgaaaacaga atcattgaat tagaagaaaa aattgacaac cttataaaaa
    12181 actataaggg tatgaaagaa gagcatgaaa agctcctggg tagagtgaaa tcgctcgaaa
    12241 ccgagaacac tgacctgaaa accaagatgg ctgatgttaa gaacgaacgc gagctgttaa
    12301 tcgagaaggt tacgaagata ctcgagaagg ttgacaaggt agaggcgtaa gaaggtttga
    12361 tgcagcgaaa ggttgaggta agtatattga atcaaccatt tacctttatt ggagacgatg
    12421 aaggaagaat aaaaagagca gcacaatttg ttgatgaaaa gataggcttt gtcatcaaca
    12481 actatggtat agtgaataca atgaatgcga tcataatggc aatgatggaa ataagtgacg
    12541 aatacttaga gatgagaaaa aaatctgagc agtttgaagg gagggcatta aagctcttac
    12601 aaaaatttga agaatttgag gttccctgcg gtgcccgtga taaatggtaa gcttcgagcc
    12661 aacacctctt tattgggggc cttcactaat tgcggtgtgc atgctcgtca tgacgagaag
    12721 cctgatgcaa caacaaggtg cccacgtaaa ccagaggttc aagattttac tgtttacacg
    12781 gcatctgcgg gggtaatctt acaacggggt agttgttgag gcaatagagg tttaacataa
    12841 atagttttcg gtagtttggg ggcatgaaat atagtttcac tatttgacat agacacataa
    12901 tttccctttt acctcatcag ccttacccct tgtatacata tatatgaggg ggaatcttga
    12961 atataaatat cataataata attgtaagtg tcgtaatagc gctttgtttc ggatttgtac
    13021 ttgctagaat catacagaga aagaagatcg gtgagtacga aaagatagga aaaaaaatcc
    13081 tggaagatgc taagaaggag gcagatgttc tcacccggga agcgtcaatc caggccaaag
    13141 atgttgcact tcaggcaaaa aacgagttgg aacaggaaat aaaatccagg cgtctggaac
    13201 tgcaaaactc tgaaaggcga cttgttcaaa aagaatcaag cctcgataag aagacagaac
    13261 aaattgagaa aaaagaagaa gagcttgcaa agagggaaaa agaagtttcc agcagacagt
    13321 catactttga taccagaatt aaagaacagg acgctttaat caaagaaggt aaagcaaggc
    13381 tcgaaaagat ctcgggaatg acagcagagg aagctaaaaa aactttgatg caggcaatgg
    13441 aaaacgaagc gaaatatgag gccctgaaga tatgcaaaaa aatagaagaa gaggcccgag
    13501 aaaaggctga tagagaggca aaaaagatta tcgctcttgc cattcagcgt tatgctgggg
    13561 attatgtggg agaggacaca gtctcaacag ttccacttcc aaacgaagag atgaagggaa
    13621 gaatcatagg tagagaggga agaaatatca gggcccttga ggctgctacc ggtgttgaca
    13681 ttattatcga tgatacccct gaggcggtta tactatcctg ttttaatcca attaggagag
    13741 aggttgccaa aatcgctatc tcaaggctta ttagtgatgg cagaatccat ccggcaagga
    13801 ttgaggagat tgtttcaaaa gtggctgaag agatggagga ccgaatcaag gaggccggag
    13861 aacaggcagt ttttgacctc ggtgttcata atgtccaccc tgagatcgtt aaacttcttg
    13921 gaagattgac ctttcgaagc agttatgcac agaatgtcta ccagcattca ttagaggtag
    13981 cttttatctg cggtgtaatt gcatctgagc tccgtgtaaa tgttaaagag gctaaaagag
    14041 ctggtttgct tcatgatatc ggtaaagcag tagatcacga gatagaaggt tcccacgcct
    14101 ctatcggggc agatcttgcc cgaaaatatg gagaaaatga aactattgtt catgcaatca
    14161 tgtcccatca tgaagacgtg gagccaactg gaatccttga tgttattgta caggcagcag
    14221 atgcactctc aggggcacga cccggtgccc ggcgagaaat gcttgagagc tacataaaga
    14281 gactagaaga acttgaacga atagcaaaca atttcccagg ggtcgataag tcttatgcca
    14341 tccaggcagg acgagagata cgcattgttg tcaatagtga gaagatcaac gatgataaca
    14401 tctatctttt gtcacgggat attgccaaaa agatagaatc tgatctctca tatcctggtc
    14461 aaatcaagat agttgtaatc agagagacac gggcaataga atatgccaaa tgagattaaa
    14521 gtcttattcc tgggggatgt cattggcaaa ccaggaagga aggctgtcga ggagtttgtc
    14581 aaaagcttta aagctgattt tgttgtaatt aatggggaaa acctagcagg gggaattgga
    14641 ataacaccac atgtagccca ggagatgttc tcatgtggtg tggatattat cactacagga
    14701 aaccatgttt ggaagaaaaa ggagatgatt ccttttttaa tggaagaaca aagagtgctc
    14761 cgcccattga actatccatc tgggacccca ggttttggcc atagcatcac ccgcaagaac
    14821 gggaaacgga tttgcatcgt taatctcgaa gggagggtgt tcatgacacc ccttgattgt
    14881 cccttcaggg caataggtaa tcttctgaag gagttggatg acaaaatccc aatcatcatc
    14941 gacttccatg ctgaggcaac atcagagaag attgcccttg gttggtatct tgatggaaaa
    15001 gtcgctgctg ttgtgggcac ccatacccat gtacaaacag ctgacgagag gatcctacca
    15061 aaaggcactg gctatatcac agatgcaggt atgacgggcc cctgtgattc tgtgattgga
    15121 atggaaaaga gtgtagtcct taaaaagttt gttacccaat taccccaaag gttcgaagtg
    15181 gggaaaaatg atattgaagt gcagggggtt gtgttaacat tggagaatga aggaaatagc
    15241 tgtatcgcaa ttgaaagaat aaagcaaaaa atagtgtaag tgaaaaaagc gagacgtaag
    15301 aagaaaagat agaacattct aaaaatacca attagttctc gttggtacat ggtttctttt
    15361 ctcaaacggg gtgggatatg tttgatgttg acaaacagat ggaagttata aagcggggag
    15421 cagtcgatct tgtaaatgaa gaagaattga gaagcaaact tgagaggtcc ataagggaaa
    15481 ataagccctt acgggtgaag cttgggcttg accccaccgc gccggatctg catcttgggc
    15541 atacagttgt acttcaaaag ctgaaacaat tccaggagtt agggcatgta gctattttcc
    15601 ttatcggaga ctttaccggc atgattgggg atcctacggg aagaatcgaa acaaggcctg
    15661 cgctgacaaa agatgagctt cttgtcaatg cagagacata caaaaaacag gtatttaaga
    15721 ttcttgatcc ggaaacaaca gaggtacgat ttaatagcga gtggtttgaa accatgaatg
    15781 ctgcagatat gatcaggctt tgcgctcaat atactatggc aaggatgatg gaacgagaag
    15841 attttagaaa ccgttaccaa aataatcttc ctatcagtat ccatgaattc ctttaccccc
    15901 ttgtgcaggg ttatgactct gttgcccttc aagctgatat cgagcttggt ggccacgatc
    15961 agatatttaa cctctttgtt ggaagagtca ttcagagggc atatggccag gagtcacaag
    16021 tccttgtcac tgtgccactt ctcgaaggaa cagacggcat taacaagatg agtaagagct
    16081 atggaaatta cgtgggaatt gacgaacaac ctgataccat gttcggtaaa ctcatgtcaa
    16141 tctcagatga actaatgctc aaatattatg aacttctcag cgatatatca ttagataaac
    16201 ttcgtattct caaggaagag attcacaacg gatctgtaca cccaagagat gccaaagtca
    16261 actttgcaaa agaaataatt acaagattcc acagtaaaga ggcggctgat gcagcccatg
    16321 agaacttcga taagatgttt cgggataagg aagtgcctga agatattgaa gtggttacta
    16381 tcaaaaaatc agaaaccggg acatggttgc caaaacttct tgccacgatt ggcatggtta
    16441 catccacctc ggaaggaaaa cgaatggtcc agcagggcgg tgtccatatc aatggagtga
    16501 aagtgaactc agaggaaata acccttgatg ctgtcaacga gttaaccata aaggttggaa
    16561 aaagaaaatt caaaaaggtc atatttgaaa atgaaagtta gtatgccttg acacaaagga
    16621 tattttgttg tttacttata cgctcagagt gatggtggta aaagctttaa atagtatgtg
    16681 ttcttttgta aaacacgcac tccccagtag ctcaatcggc agtagcggtc ccgttgtaac
    16741 gggataggtt gcaagttctg g
//
LOCUS       DAOOMC010000061        16399 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdus sp. isolate AMR_MDS_2685
            NODE_3735_length_16399_cov_6.311490, whole genome shotgun sequence.
ACCESSION   DAOOMC010000061 DAOOMC010000000
VERSION     DAOOMC010000061.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864760
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdus sp. (wastewater metagenome)
  ORGANISM  Syntrophorhabdus sp.
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae; Syntrophorhabdus.
REFERENCE   1  (bases 1 to 16399)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16399)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,729
            Genes (coding)                    :: 2,708
            CDSs (with protein)               :: 2,708
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 7 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16399
                     /organism="Syntrophorhabdus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3735_length_16399_cov_6.311490"
                     /isolate="AMR_MDS_2685"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2699756"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..823
                     /locus_tag="PK125_07590"
     CDS             <1..823
                     /locus_tag="PK125_07590"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HPB38006.1"
                     /translation="GKEVVFNLVHQLSSRRNQPLVKVNCAAIPETLLESELFGYEKGA
                     FTGAFQRKIGKFELANKGTIFLDEIGDLTPLLQAKLLRVIQEKELERLGGLHPVKIDV
                     RLIVATNRNLEEEVKKGSFREDLYYRLNVVKIVVPPLRERKEDIPLLMDFFVKKYATR
                     YKKNIVGFTRESRDQLIKYDYPGNVRELENMIERAIVLARGDHITLEDIPHLSESNIV
                     QAEGGIKETVGVVEKQMIMEALIKADWVQTRAAGFLGISERMLRYKMKKYAITRE"
     gene            827..1978
                     /locus_tag="PK125_07595"
     CDS             827..1978
                     /locus_tag="PK125_07595"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HPB38007.1"
                     /translation="MKVTKRAELITPFYVMELLEKAKEMEARGEDIIHMEVGEPDFTM
                     PQLVKEEAIKAIISNETYYTHSLGIHELRQKISTFYKKSEGISVSPECIVITSGTSGA
                     FLLLAAVLLERGRTLALSDPGYPCYRNFALLADADVISLPVNAESGYQVMPDQIANLR
                     NGPDVLIICNPSNPTGSIYHEETIAQLHEIISCRNGVLVVDEIYRGLTYGRTLRTALH
                     VNRDIIVVNGFSKTHAMTGWRLGWMVVPQELVRPIQKVAQNVFISPPSIAQHAAVHAF
                     DVSEYLEEMRTTYEERRNFILPRLKNLGFTVPIDPEGAFYVYAGIGKWGLDSMRFVER
                     ALVEAKVAITPGYDFGSYMARSHVRFSYANSLERLKEGCNRLENWLQTV"
     gene            2558..3397
                     /locus_tag="PK125_07600"
     CDS             2558..3397
                     /locus_tag="PK125_07600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB38008.1"
                     /translation="MKKTLVCLVVFTVLFLVANICVAQAQVTGLNGTWLKFTGSLKGM
                     RFTNGPGSEELGRNDKDSIRLYGCVVDSPYFFDNQFFVQLYKSKRNAIRAGAAVFQKP
                     DGTADNFAGWFNMNLMDEYNPATPNVYSTSISTPGQMTIRRDKIKFKGFGGQAEKIGG
                     GYTGYALYGVKNMSAKTVNENNVPLSKYISCPAGYVINVKNTDDGDHTGTITPPGPWV
                     DVTTGYSQVFNIKVNAPGTYVYVYIDNELKYYYPDVYPEDTIPPVTIDSDGENHSLWV
                     HFD"
     gene            complement(3470..4231)
                     /gene="mtgA"
                     /locus_tag="PK125_07605"
     CDS             complement(3470..4231)
                     /gene="mtgA"
                     /locus_tag="PK125_07605"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: protein motif:HMM:TIGR02070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HPB38009.1"
                     /translation="MKLAKIVFFFVILSFFTGIGFFMIYPDVSKLMKENPKKTSFMEY
                     REEEWEKKGKRIKIQKKWVPLSHISPYLIKAVLIAEDDKFWSHKGFDLDAIRKALEKD
                     LDKGKFKFGGSTISQQLVKNLYLTPSKNPLRKAKEAIITWRMEKALSKRRILELYLNI
                     VEWGEGIFGIEVAAQHYYGKPSGALSPQESARLAAVLPNPRRYRPNRPSRYVDKRAAI
                     IYDIMVKRGIVVPDYEAVINEEVTLDSSLPLDHNR"
     gene            complement(4317..5246)
                     /locus_tag="PK125_07610"
     CDS             complement(4317..5246)
                     /locus_tag="PK125_07610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB38010.1"
                     /translation="MTKTGDAVIPKKAMTIILLRDQYPAGFEVLLLKRHNQSIFMGGN
                     YVYPGGKVDEEDATEEILARSQGISSRQAQEILGENDAKIAIAYWIAGIRELFEEAGI
                     LLAYNKEKRLLSIDSEDAKQRLAFHRHSLQKGEKSFLQIIIEEDLTLAIDQCFYYAHW
                     ITPEARNVRFDTRFFVARTISGQEASPDAKETTEGVWLTPSAALQKNFEGTVALSPPT
                     LKTLEDLSRFSSIHDLLSSLPERKKPAILPILLNPLADETLIFPWDPEYEDLRSGTLQ
                     QSKDHGLPSSPLQNTTRVVLTDGRWLPYVKTVL"
     gene            complement(5320..6321)
                     /locus_tag="PK125_07615"
     CDS             complement(5320..6321)
                     /locus_tag="PK125_07615"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HPB38011.1"
                     /translation="MIASLQSEDWESRLQAAENLGKSKYKKAIEPLIQALNDSDEYVR
                     EGAVWSLGEIKDKRAIPALIKALNDQDQYVREGAAIALGKLKDKRAVGPLFQLLRSDS
                     ESMRRTAETALGTLGECATEPLLDMLHDPDWDVRWRATHILGEIKDLRAVEALIEALK
                     DENKYVIEGAITALGEIKDKRAVEPLMEALKDTNKYVREEATNALGEIRDERAIHALV
                     DVLSDKDWGVRWGAANALGEIRRKGAIEPLIHAFSDEVRYVRESAAAAVVRIGKKAGG
                     HLVQALGSSDPNVRRWAAWTLGKLKYEKAAEHLALALQDEDGGVQFAAEVALAEIEG"
     gene            6657..7418
                     /locus_tag="PK125_07620"
     CDS             6657..7418
                     /locus_tag="PK125_07620"
                     /inference="COORDINATES: protein motif:HMM:NF013077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SagB/ThcOx family dehydrogenase"
                     /protein_id="HPB38012.1"
                     /translation="MNQKKKQPDFMKPGIGNQFQEETKYAPDRIGNYSLNWKTFPEPF
                     KNYDSPIAVIGLSQPEFAGVQNIWEALQKRRSRRVFRSTDPLAMSDLSTLLWATQGIT
                     EKYGETLFRTAPSAGGLFPVETYLNVRLVEGLEEGIYHFRPGKFDMEFLKKGKFSRLL
                     AQAALGQEIVATAQVTFIWSAVLERSKWKYRQRAYRYIYLDAGHICQNLYLAGEAVGL
                     GVCAIGAFFDDDINEIVGLDGTDETIIYMAAVGKV"
     gene            7698..8243
                     /locus_tag="PK125_07625"
     CDS             7698..8243
                     /locus_tag="PK125_07625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB38013.1"
                     /translation="MINLNDLLTPDSADEILVSSALEDESLVFFAGPLSEVSDLSITG
                     PAVYGSGTQVKSSKAKMKVLTCHVTTSTTNTMKTIFPWVSTGVKVAHIAYFEILNKSD
                     AVKFKVRVQGPEFSQPLVVETLTFGPQEPLYQWGIGFWRSYSVPGIYKVKMTAIPQTN
                     KMSGRSSIECSFRVSSSQTPE"
     gene            8367..8645
                     /locus_tag="PK125_07630"
     CDS             8367..8645
                     /locus_tag="PK125_07630"
                     /inference="COORDINATES: protein motif:HMM:NF016839.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HPB38014.1"
                     /translation="MIEEKIRKYGLVVVLAILFFHFLFSDGGLMSYLKVKRGIKAANT
                     SISVLDKENTILKKEIEKLQKDDEYLEEVVRKKYGFLREGERLYRIEK"
     gene            8642..9517
                     /locus_tag="PK125_07635"
     CDS             8642..9517
                     /locus_tag="PK125_07635"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HPB38015.1"
                     /translation="MRYEGSIYRPPSEADSLILQVTIGCSHNKCTFCGSFKDKKFRVR
                     SFEEVKEDVEEAKAYARYVRKVFIADGDALIIPQKMLVPIVQLIKDSFPKLERIGIYG
                     NTKSILKKSVEELKELKKLGIGIIYLGVESGDQITLDRVHKGTKLDKTAEASKRVKDA
                     GIILSVTVLLGLGGVERSTVHAEETGKFLSRIDPDYAGALSVIVVPGTPLAEEVKRGT
                     FQVPTPYQLLEELYIMIKNTNVTHTFFASNHASNYLPVKAWLPEDKEKTLKAIQYVLD
                     KKDPALLRPEYMRAL"
     gene            9519..9959
                     /locus_tag="PK125_07640"
     CDS             9519..9959
                     /locus_tag="PK125_07640"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024675.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HPB38016.1"
                     /translation="MSQSADGRRNRTVDYVETPIRVRYADTDTMGVVYYGTYPIYFEV
                     GRAEYMRSKGIPYKRFEETGFHLVVVHLEAKYYNTAAYDDLLTVRTSITELKSRGLTF
                     HYEIFRDGDLIVQGKTRHICTNSDKKTVVMPVSLFEILKDVNPQ"
     gene            10000..11007
                     /locus_tag="PK125_07645"
     CDS             10000..11007
                     /locus_tag="PK125_07645"
                     /inference="COORDINATES: protein motif:HMM:NF013257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HPB38017.1"
                     /translation="MTIPAVKALKEYYPHAHLTWLVEGSVGELLTHQGFIDEVIRFPR
                     GSIQSAMKKGNLTSATKQTKCFLKKLRGGEYDLILDFHGIIKSALFSKMVKGKRIIGF
                     GKTLAKEKSHLFYHECVECENKRLHKMERNMLIPRHIGVDMTIPAVKLESPPDAESYI
                     DEFFTRERVVSPIFAINPFSSKGSAFKRWEMERYAELIRRITAENIGKVIILWGPGEK
                     GEAQRLKKMAGEEALLSCSTDVPQLLALLHRVDMYVGGDTGMMHLAALAGTPVLAIFG
                     PTDHKVNGPYGPIHRIIRKDLTCSPCRNKNCQERRCLTGIAVDEVLREVIELYEKIKG
                     N"
     gene            11008..12510
                     /locus_tag="PK125_07650"
     CDS             11008..12510
                     /locus_tag="PK125_07650"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HPB38018.1"
                     /translation="MRVLFIYPNLNAQIGFNYGLAYISGILIKKGVETHLLNVNEQIG
                     YPLDLERIRTDVLRIKPDIVGFSVLTNQYKYSLEIARDIKKYLDVRIIFGGIHPTMDP
                     EGTLAESCVDYLCIGEGEEAFLELVEKGSPEGIRNLAYKENGRMVIEPLRPYVDVTKL
                     PFKDYHLFDFQKMIDAKDGWVGLQASRGCPFRCTYCLNHKIIDLYKKQGHLPKDYLRR
                     HSVDEIIDEIKYLLSHYTGIKMFIFDDDIFTFDKEWLKEFSRRYKEITDIGFVCNAHA
                     RIFDSETAKNIKEAGCRIVKFGLESGSDRIRRTVLQRFMSNGHIEKAFETADRHGLHT
                     SAFVMIGLPDEKKIDLMETVKLLAKIKPGRFRWSLFFPFIGTRAYTIAKDLGAIDFEK
                     MDRLDNFTDETCMNLGPDEDLLIDKLKTLFCLFVNGYADIDGKGKYAGLLREVEDADE
                     AAWQRNKTKFSERLKTLDREMANHGTIHYTIKYNRFMGVRSDWQDDSLSS"
     gene            12479..13486
                     /gene="waaF"
                     /locus_tag="PK125_07655"
     CDS             12479..13486
                     /gene="waaF"
                     /locus_tag="PK125_07655"
                     /inference="COORDINATES: protein motif:HMM:TIGR02195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008920 - lipopolysaccharide
                     heptosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase II"
                     /protein_id="HPB38019.1"
                     /translation="MTGRTIVYLPNWLGDMVMATPFLHSLRAHVKEELWAIGKNKALQ
                     LYNGNSLFDRFIPYDGKGVVPFLDTVSFIRALNFRDGVILPHSFRSALLFFAGDVQQR
                     IGYARNQRGFMITLKVEEVTRPEPTVEHYLKIIDAMGGTRVLTTPLLEVTEEEDKRFD
                     EKYVDLNAPYVVFITGAQYGPAKRWPDHYFAELANKIARELDMNVYLLPGFGEEELAQ
                     KIYQNVEHKKRVFVKVMDIMELKVCLSRAAAVVTNDTGPRHISAALSVPTVVLLGPMD
                     EQYTLYPSSCTYVFSKDVACRPCNKKRCDKNHVCLTSISPNDVFQKLEEILEVRPSKA
                     H"
     gene            13458..14450
                     /locus_tag="PK125_07660"
     CDS             13458..14450
                     /locus_tag="PK125_07660"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional ADP-heptose synthase"
                     /protein_id="HPB38020.1"
                     /translation="MKSDRVKLIERFDGRKICVVGDIIADIYIFGRPYRLSREAPVVV
                     VQHEEERIYPGSAGNTINNLLALGAHVFPLGFIGSDSTGDQLIDFFLRFNKVEMDGFI
                     RHNGETITKTRILAGDTHTSKQQVIRIDRDSNKLPTKSQKNLLYKKLKEIGPSMDAFI
                     VSDYGHGTVDETIIDYMQHVAKKTIVVGDARYRLRDFKGFTLITPNESEAYQLCSTDF
                     DKDIEEVGRRILKIMDVTALLITRGNKGMSLFLKEGSVNHIPISGNDDVTDVTGAGDT
                     VCAAVTLALASGADFLTAALIANYAAGVVVMKRGTATLTQEELRAAMQAYGTNR"
     gene            14434..14910
                     /locus_tag="PK125_07665"
     CDS             14434..14910
                     /locus_tag="PK125_07665"
                     /inference="COORDINATES: protein motif:HMM:NF013621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyltransferase/cytidyltransferase family
                     protein"
                     /protein_id="HPB38021.1"
                     /translation="MGQIVKKLDELKSIVEKEKNQGKKIVFGNGCFDLLHVGHVRYLK
                     GAKALGDVLIVAVNDDSSVMGLGKRKRVVTPAPERAEIIAALEGVDYVILFSDPNVEN
                     LLRTLQPHIHAKGTDYTEESVPEGRIVRDYGGRVAIVGDPKDHSTRDLIKAIKDMD"
     gene            14940..15782
                     /locus_tag="PK125_07670"
     CDS             14940..15782
                     /locus_tag="PK125_07670"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HPB38022.1"
                     /translation="MDQPVASNQQKQTLNSEQLTPSDGLPLSVYMITYNNGVTIERAL
                     QSVADWADEVIVVDSYSTDGAQDIIPRYTEKLYQFETKDLREKYQYAHDQCINPWTLF
                     IDADEWLMDDIKKEISTIIRGTPSCDGYIASRRNMYLGREIKHGGWYPDREIRLYRRE
                     KGRWEGGLHAKVTVDGTVGTLKHFYMHTPYADIAHQIRTIDRYSEAFAEDLRSSGRRF
                     HLVNLITRPVYRFFRDYILKRGFLDGTPGFIIVVSTMYYVFMKYAKLWEIEMKEKKQF
                     RDRF"
     gene            15845..>16399
                     /locus_tag="PK125_07675"
     CDS             15845..>16399
                     /locus_tag="PK125_07675"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF013530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HPB38023.1"
                     /translation="MNTFKDKEVLITGGLGFIGSNLTIDLVELGAHVTIVDNMLPRQG
                     SNLFNIKEVEDRVRVNISDIRNQLSMNHLVKGKDYIFHLAGQVNHVDSMRNPLQDLEI
                     NCVGTLVLLEALRHNNRDAKVIFAGTRGEYGASVKLPVNEDHPTNPKGLYAVTNLAAE
                     KMVLVYDDIFGIKSVCLRITNTYGP"
BASE COUNT         4784 a         3406 c         3823 g         4386 t
ORIGIN      
        1 aggcaaggag gtggtattta accttgtcca tcaactgagc agccgacgga atcagccgtt
       61 agtcaaagta aattgtgcgg caatccctga aacactcctc gaaagcgagc tgttcggtta
      121 tgaaaaaggt gcctttacag gcgcttttca gagaaagata ggaaagtttg agcttgccaa
      181 taaaggcaca atctttcttg atgaaatcgg cgaccttaca cccttgctcc aggcaaagtt
      241 actgagggta attcaggaga aagaacttga acggttaggt ggcctccacc ctgtgaaaat
      301 tgatgtacgg ttgattgttg cgactaacag gaatttggag gaagaggtaa aaaaaggaag
      361 ttttcgggaa gatctttatt atcggctcaa tgttgtgaaa atcgtagtcc cccccttaag
      421 agaaaggaaa gaggatatac ctcttcttat ggattttttt gtcaaaaaat atgctacacg
      481 atataagaaa aacattgtag gttttaccag agagtcgagg gatcagttaa taaaatatga
      541 ttatccaggg aatgtgaggg aactcgaaaa catgatcgag agagccattg tccttgcccg
      601 aggtgatcac ataactttgg aggatattcc gcatttatct gaaagcaata ttgtccaggc
      661 tgaaggaggg atcaaagaga ctgtaggtgt tgtggaaaaa cagatgatca tggaagcgct
      721 catcaaggca gactgggttc agacaagggc agcggggttt ctcggtataa gtgaaaggat
      781 gcttcgatac aagatgaaga aatatgctat aacccgcgag tgagacatga aggttacaaa
      841 acgggcagag ctgatcaccc ctttctacgt aatggaactc cttgaaaaag cgaaagaaat
      901 ggaagccagg ggagaagata tcatccacat ggaggtgggt gaacctgact ttacaatgcc
      961 gcaactggta aaggaagagg ctatcaaagc tataattagc aatgagactt actataccca
     1021 tagccttggg attcatgaac ttcgacagaa aatttcaaca ttttacaaaa aaagcgaagg
     1081 gatttctgtc tcaccagaat gtattgtcat taccagtgga acctctggag ccttcctgct
     1141 tcttgccgct gttctccttg agaggggaag aacgcttgcc ctttccgacc cagggtatcc
     1201 ctgttacagg aattttgccc ttctcgcaga tgcagatgtc atctctcttc ctgtcaatgc
     1261 agaaagtgga taccaggtca tgcccgatca gattgccaat ctcaggaatg gtcctgatgt
     1321 gcttatcatc tgcaatcctt ccaaccctac gggatccatt tatcatgaag agacgattgc
     1381 gcaactccat gaaatcatat cctgccggaa tggagtgctc gtcgtagatg aaatctacag
     1441 aggactaaca tacggacgga cattgcgaac tgctctccac gtgaatcgag atatcatcgt
     1501 ggtcaatgga ttctcaaaaa cccatgccat gacaggatgg cgccttggat ggatggttgt
     1561 gccccaggag ttggtccgac ctattcagaa agttgcacaa aatgtattta tttcaccacc
     1621 ctcaatagcc cagcacgctg ctgtccatgc ttttgatgtt agcgaatatc ttgaagagat
     1681 gaggaccacc tatgaagaac gacgaaattt tatccttcct cgtctgaaaa acctcggctt
     1741 taccgttccc attgatcccg agggtgcttt ttatgtctat gcagggatcg ggaaatgggg
     1801 tctggacagc atgagattcg ttgaacgggc acttgttgaa gcaaaagtag ccattacgcc
     1861 gggctatgac tttggaagtt atatggccag atcgcatgtt cgtttttcct atgcaaatag
     1921 ccttgaacgg cttaaagaag gttgcaacag gttggagaat tggctgcaga ctgtgtagac
     1981 tccagttatc caacaggctg aatcccacaa atttggcaaa taacactcta tgttgtcata
     2041 attggttgtt gcatcacaaa ccttattttt ataaatagtt cccaaaaaaa tacttctgct
     2101 attttaggta tgtagacatg cctaaaaagg tcttaacacc ccagcctgtt ctttgaactg
     2161 ataaaggtaa tgatatttcc tggatgctat tcaggacagc aataccacca catggaatgt
     2221 catgctccag gcgctcttgc attcggttct gttactatct tcaatggcaa tttcataaag
     2281 ggcgcagagg tctcatttga tgttagccca tgcaaaagcg taaccggctg tgaggagtcc
     2341 agcctctttc cttaggacaa tctaacaaat acggtaagta atggaataag atggctggcg
     2401 aactgtcaaa gaaaagataa aaaaagcttt gtaaagggta ttgaaggaag cacgtaagga
     2461 ctggtgaaat gtaatggcct gtcagaaaag gatgtgatag aatactacgg gaataaactt
     2521 cccattaccg aaataatagc tggcaaggag gtttaatatg aaaaagacct tagtgtgttt
     2581 ggttgttttt acagttcttt ttttagtagc aaatatttgt gtggctcaag cccaggtaac
     2641 tggactaaat ggcacgtggt taaagtttac tggaagcctc aagggaatga ggtttaccaa
     2701 tggccctgga tcagaggagc ttggaagaaa tgacaaagat tctattcggt tatatggatg
     2761 tgtggtagac agcccttatt ttttcgataa ccagtttttt gtgcagttat ataagagcaa
     2821 aagaaatgcg atcagggctg gtgcggctgt tttccagaaa ccagatggca ctgcagataa
     2881 ttttgccggt tggttcaata tgaatctgat ggatgaatat aaccctgcca caccgaatgt
     2941 atatagtacg tctatttcca cacctggtca aatgaccatc aggagggata agataaagtt
     3001 taaaggcttt ggcggacagg cagagaaaat aggcgggggc tacacggggt atgcgctata
     3061 cggtgtgaaa aacatgagcg ccaagactgt aaatgaaaat aatgtccccc tcagtaaata
     3121 catttcgtgc cctgctggct atgtaatcaa tgtaaaaaat acggatgatg gtgatcacac
     3181 tggcactatt accccgccag gtccctgggt ggacgttacc actgggtata gtcaagtatt
     3241 taatattaaa gttaatgctc caggtacgta tgtctatgta tatatagaca atgagttgaa
     3301 atattactac ccggatgttt accctgaaga taccatccca cctgtaacca ttgactctga
     3361 tggcgaaaac cattcgctct gggttcactt cgattaatca tacccgtcag aagcgacatc
     3421 atgtccatgg gtaacacccg tggacatgat tttttaaaat tggcagaatt tagcgattat
     3481 gatccaacgg caaagagctg tcgagcgtaa cctcttcgtt tataacagct tcatagtccg
     3541 gtacaactat acctcttttt accattatat cgtatatgat agcagcccgt ttatccacat
     3601 atcttgaagg tctgttgggg cgataccttc tggggttagg cagcactgca gcgaggcggg
     3661 cagactcctg ggggctaagg gcacccgagg gtttgccata ataatgttgt gcggcaactt
     3721 cgatgccaaa gatgccttcg ccccactcta caatattcaa ataaagctca agaatccttc
     3781 ttttgctgag tgctttctcc atgcgccagg taataattgc ctcttttgcc tttcgtaaag
     3841 gattcttgga tggtgtgaga tagagattct tcacaagctg ctggctgata gtactccctc
     3901 caaacttaaa tttgccctta tccaggtcct tttccagagc ctttcgaatt gcatcaaggt
     3961 caaaaccttt gtgagaccag aatttgtcat cctctgcaat gagcactgct tttatgagat
     4021 atggggagat gtgagataaa ggaacccatt ttttctggat cttgattctt ttccctttct
     4081 tttcccactc ttcttcgcga tattccatga aagatgtctt cttagggttt tctttcatga
     4141 gcttggaaac atcagggtag atcatgaaaa aaccaattcc agtaaaaaat gacaggatta
     4201 caaagaaaaa gacgattttg gcgagtttca tgatgttatc actggggtct taatgtaccg
     4261 tttgtatgtc tcatatagcg ttttaccttt tcttgctgca tactaaatgg gatatactac
     4321 aaaacggttt taacatacgg cagccaacga ccatctgtca acacaacgcg tgttgtgttt
     4381 tgcaaagggg atgaaggcaa gccgtggtct tttgactgtt gtaatgtgcc ggaccgcaag
     4441 tcttcgtatt caggatccca cggaaaaatg agcgtttcat ctgccagggg attcaaaaga
     4501 atagggagaa tggcaggttt tttcctctca gggagtgaag aaaggaggtc atgtatggat
     4561 gagaatcgtg acaagtcctc cagcgttttt agggttggag ggctgagcgc tactgtgcct
     4621 tcaaagtttt tttgaagggc cgcggaaggc gtcaaccaaa ctccttcagt cgtctctttt
     4681 gcatcagggc ttgcttcttg gcctgatatg gttcgggcta caaagaaacg ggtatcaaac
     4741 cggacatttc tagcctcggg ggtgatccag tgggcatagt aaaaacattg atcgatggca
     4801 agcgtcaaat cttcctctat tattatctgc agaaaacttt tttctccttt ctgaagactg
     4861 tgccggtgga aagcaagtcg ctgctttgca tcttcgctgt caatggaaag aagccgcttc
     4921 tctttgttat atgcgagaag aatccccgct tcttcaaaaa gctccctaat gcctgctatc
     4981 caataagcga tggcaatctt agcatcattt tccccaagaa tctcttgggc ctgccttgaa
     5041 gagataccct gtgaacgggc aaggatttct tcagtagcat cctcttcgtc aacctttcct
     5101 cccgggtaaa cataatttcc acccatgaaa atgctctgat tatgtcgttt gagaagaaga
     5161 acctcaaatc ctgcaggata ctgatctctg aggagtatga tggtcatagc ttttttgggg
     5221 attactgcat cacctgtttt tgtcatttta atatctttca aaagatgtaa aaggaggcta
     5281 agggtaagga tctgctattg aacaagtctg accggtatgt cagccctcaa tttctgcgag
     5341 agctacctct gccgcaaact gaacgccgcc atcctcatcc tgcaaagcaa gagcaaggtg
     5401 ttccgcagct ttttcgtatt tcaattttcc aagagtccag gctgcccagc gacggacatt
     5461 tggatcagag cttcctagag cttggacgag atgtccgcca gctttcttac ctattctcac
     5521 gacagctgca gcagcgcttt cccgaacata ccgaacttca tcagaaaagg catgaataag
     5581 gggctctatg gcgcctttgc gccgtatctc gccaagagca tttgctgcac cccaccgcac
     5641 accccagtct ttatcagaga gcacatccac gagggcatgg atggcccgtt catcccttat
     5701 ttcacccagt gcattggttg cttcttccct cacatatttg ttagtatcct ttaaagcttc
     5761 catcagtggc tctacagcac gtttatcttt gatctctcca agggcagtta tggcaccttc
     5821 tataacatac ttattctcgt cttttagcgc ttcgattagt gcttctacag cacgaagatc
     5881 ttttatctcg ccaaggatat gggtggcacg ccaccgaaca tcccagtccg gatcatgcaa
     5941 catgtcgagg aggggttcag tcgcacactc gcccaaggtg ccgagtgcag tctccgcagt
     6001 tcttctcatt gactcggagt cacttctgag gagttggaaa aggggcccca cagcccgttt
     6061 atctttcaat ttacccaatg ctattgctgc gccttccctg acatactggt cttgatcatt
     6121 caaggcctta atgagtgccg gtattgcacg cttatctttg atctccccga gagaccaaac
     6181 cgcaccttcc ctgacatact catcgctgtc attgagggcc tggatcaacg gctctatcgc
     6241 ctttttatac tttgacttcc caaggttttc agctgcctga agtcgggatt cccagtcctc
     6301 gctctggaga gatgcaatca agttgtctat ttcatttttc tttttcagtt gagcacctgc
     6361 tactatactt ttatcatatc aaaaagtttt aagaaacaaa attaatttaa aagctgatcg
     6421 agaagcaagg gtaacgggtt atacaggtaa atactggcac aaggccatga gaaatagacc
     6481 ataagcaaaa aacattgatt agcaactgat attgttcagc atttttctat cacctaccat
     6541 aaatggcctg tcacattgcc tctttctcgt aagttgacag cgcgtatagt tgtgaacata
     6601 atacaaatgg tagtcattag acatttgtct tatccagacg tcttcggagg gcaataatga
     6661 atcaaaaaaa gaaacagcct gattttatga agccaggcat tggcaatcaa ttccaagaag
     6721 agactaaata cgcccctgat cggataggaa attattctct taactggaaa accttccctg
     6781 aaccgttcaa gaattatgac agtcctatag ctgtaatagg tctttcacaa cctgagtttg
     6841 ctggtgtgca aaatatctgg gaagctttac aaaagcgcag atcaaggagg gtttttagaa
     6901 gcactgatcc ccttgctatg agtgaccttt ccacactttt gtgggcaacc cagggaatca
     6961 cagaaaaata cggtgaaaca ctattcagaa cagccccttc tgcaggagga ctttttccag
     7021 tggagactta cttgaatgtt aggcttgttg agggtcttga agagggtatt taccatttca
     7081 ggcctggcaa gtttgatatg gagttcctta aaaaagggaa attttcaagg ctactcgcac
     7141 aagctgccct tggccaggaa atcgttgcaa ccgcacaggt aactttcatc tggtcagcgg
     7201 ttcttgaacg gagtaaatgg aaataccgac aaagggccta ccgatatata tatctcgatg
     7261 cgggacacat ctgtcagaat ctctatctgg caggagaggc ggtcggatta ggcgtctgtg
     7321 ccataggcgc gttttttgat gatgacatta acgagattgt aggccttgat ggcaccgatg
     7381 agaccatcat ctatatggcg gcagtaggta aagtataggg atatcaacaa acccccaccg
     7441 aagatttctt ccaacagaaa ctgatgattt atctacaatt atcgttgcca tgacataatt
     7501 caaacaagcc tatcatattg aattcattta taattatttt aggcattcaa tgtgcattac
     7561 aagtaacgta tgaaaaaaaa tgagaaagga aagtagccgt gagaaaaaca agtctactgc
     7621 tagtattaac gctcttaatt ctgttggtct gtcctttatg ggtttttgca caagttgttc
     7681 gggatcaacc ggttcaaatg atcaatctta acgatcttct aacacctgac tctgctgatg
     7741 aaatactcgt aagctcagcc ctggaagacg aatcccttgt tttttttgca ggtccgcttt
     7801 cagaagtaag cgatctatca ataactggcc cggcggtgta cggttcaggg acccaggtaa
     7861 agtcatccaa agctaaaatg aaggtgctga cttgccatgt cacaacaagt actacaaata
     7921 caatgaaaac aatttttcca tgggtttcaa caggagtaaa agttgcacac attgcttatt
     7981 ttgaaatact caataaaagt gacgctgtta aattcaaagt tagagtgcag ggcccggaat
     8041 tttctcaacc tctcgttgtc gaaaccttga cctttggacc acaggagcct ttgtaccaat
     8101 ggggtatagg cttttggaga tcctactcgg taccaggaat ctacaaagtt aaaatgacag
     8161 caattcctca gacaaacaag atgtcaggtc gatcgtctat agaatgcagt tttcgtgttt
     8221 cgtcatccca gacccctgag taatcatatg tagttttatg cagtcttaaa cagaactcac
     8281 cgccttaagt ctattgctcc atgcccaaaa tgttttccat ttcttgacat gttgtcttgc
     8341 agtcagtaat atgttaccat acatcaatga ttgaagaaaa aatccgcaag tatggacttg
     8401 tcgtagtttt agcaatcctt tttttccatt tccttttttc cgatggtgga cttatgagtt
     8461 atctgaaggt taaacggggg attaaggcgg caaatacttc cattagcgtt cttgacaaag
     8521 agaatacgat tcttaagaaa gagattgaga agctacagaa ggatgatgaa tatcttgagg
     8581 aagtcgtgag aaaaaaatac ggcttcctga gggaaggaga aaggttatac cgtattgaaa
     8641 aatgaggtac gaaggatcaa tttacagacc accaagcgag gcggatagcc tcattctaca
     8701 agttaccatc gggtgttccc acaacaagtg cacattctgt ggttccttca aagacaaaaa
     8761 attcagggtt agaagctttg aagaggtaaa agaagacgta gaggaagcaa aggcttatgc
     8821 tcgatatgtg agaaaggttt ttattgcaga tggtgatgcc ctaatcatac cgcaaaagat
     8881 gcttgtcccc attgttcaat tgataaagga ctcatttccc aaacttgaac gtattggaat
     8941 atatggcaac acgaaatcta tcctgaaaaa atctgtcgaa gagttaaaag aactcaaaaa
     9001 actcggtatt ggtatcattt accttggtgt agagtctgga gatcaaataa ccctcgacag
     9061 ggttcacaaa ggaaccaaac tggataagac cgctgaagct tcgaaaagag ttaaggatgc
     9121 cggaatcatt ctttctgtca ctgtcctcct gggtttaggc ggtgtagaga gaagcacagt
     9181 tcatgctgaa gaaactggta aattcttaag ccgcattgac cctgattatg ccggtgcgct
     9241 tagtgtcatt gttgttccgg ggacacccct tgcggaagaa gtcaaacgag gaacgttcca
     9301 ggttcctacc ccttatcaat tgcttgaaga gctgtatata atgataaaga ataccaatgt
     9361 cacccatacg ttttttgctt ccaatcacgc ctcaaattat ctccctgtca aagcatggct
     9421 accggaagac aaagagaaga ccctcaaagc aatacagtat gtgttggaca aaaaggaccc
     9481 agctctttta cggcctgaat atatgagggc tctctgaggt gagtcagtca gctgatggca
     9541 ggaggaacag gacagtagat tacgtcgaaa cgcctatcag ggtcagatat gctgatactg
     9601 acaccatggg ggtggtttac tacgggacgt atcccattta tttcgaagtc ggaagggcag
     9661 agtacatgcg ctccaagggt attccctaca agaggtttga ggaaacaggt tttcacctcg
     9721 ttgttgtgca tcttgaagca aaatactaca acaccgctgc ctacgatgac cttctcacag
     9781 tcagaacaag tatcactgaa ctcaagtcaa gagggctcac attccattac gaaatattta
     9841 gagacgggga cttgatcgtc cagggtaaga caagacacat ctgcacaaat agtgacaaga
     9901 agacagttgt gatgcctgtc agcctttttg aaatcctgaa agatgtcaac ccccaatagt
     9961 atcctggcca tacgcctgag ttcttttggt gatgtgctca tgaccattcc tgccgtgaaa
    10021 gcactgaaag agtattaccc ccatgctcac cttacctggc ttgtggaagg gtctgtggga
    10081 gagttactca cccatcaggg atttattgac gaggttattc gttttccaag aggttcaata
    10141 caaagtgcca tgaaaaaggg aaatcttacg agtgctacga aacagacgaa gtgttttctc
    10201 aagaaactaa gaggcggaga atatgacctc attctcgatt ttcatggcat tatcaaaagc
    10261 gcgcttttct ccaagatggt aaaagggaaa aggataattg gttttgggaa gaccctcgcg
    10321 aaggaaaaga gtcatctttt ttatcatgag tgtgttgaat gcgagaacaa aaggctccac
    10381 aagatggaac gcaacatgct gattccccgg catataggtg ttgatatgac aatccctgct
    10441 gtaaaactgg aatcgccccc ggatgctgag tcatatattg acgaattctt tacccgggaa
    10501 agagtagtat cccctatttt tgcgataaat cctttttcca gtaaagggag cgcatttaaa
    10561 aggtgggaaa tggagcgtta tgctgagctc atcaggcgca ttacagctga aaatattgga
    10621 aaagtaatca tcctttgggg ccctggtgaa aaaggtgaag cgcaacggct taaaaaaatg
    10681 gctggagaag aggctttgct atcctgttcc acagacgttc cgcagctcct tgcactcctg
    10741 catcgtgttg acatgtatgt tggcggtgat acagggatga tgcaccttgc ggcattggca
    10801 ggcacaccgg ttcttgccat ttttggacca acagaccaca aagtcaatgg gccatatggg
    10861 cctatacaca gaatcatcag aaaggatctg acgtgcagcc catgcaggaa caagaattgc
    10921 caggaacgta ggtgcctcac gggtatagct gtggatgagg ttctaaggga agtgattgaa
    10981 ctgtatgaaa aaattaaagg aaactgaatg cgtgtattgt ttatttatcc aaaccttaat
    11041 gctcagatag gatttaacta tggccttgca tatatatcgg gtattcttat taaaaagggc
    11101 gttgaaaccc atcttctgaa tgttaatgag cagatcggtt atcctctgga tcttgaaagg
    11161 attcgaaccg atgtgcttcg cataaagcct gatattgtag gtttttctgt tctcacgaac
    11221 cagtataagt attcccttga aatagcacga gatattaaga aataccttga tgtccgaatc
    11281 atctttggcg gtatccaccc aacaatggat cctgaaggaa cccttgctga gtcatgtgtt
    11341 gattatctct gcattggaga aggagaggag gcgtttcttg aactggtaga gaaaggcagc
    11401 ccagaaggaa ttcgaaattt ggcttataaa gaaaatggcc gtatggttat agagcctctc
    11461 cggccgtatg tggatgtgac gaaactgcct ttcaaggatt accatctctt tgatttccag
    11521 aaaatgattg atgcgaagga tggctgggtg ggattgcagg catcaagagg ttgtccattc
    11581 aggtgtactt actgcctgaa ccataagatc attgatcttt ataaaaaaca gggacatctg
    11641 cccaaagatt atctccggag gcattctgtt gacgagatta ttgacgagat caagtacctt
    11701 ttatcccatt acactggaat caaaatgttt atttttgatg acgacatctt cacatttgac
    11761 aaggagtggc taaaagaatt ctcacgaaga tacaaagaga tcactgatat tggatttgtc
    11821 tgtaatgccc atgcgcggat attcgatagt gaaactgcga aaaacatcaa agaagctggg
    11881 tgcaggatag taaagttcgg tcttgagagc ggcagcgaca ggatacgaag aacagtgctg
    11941 cagcgcttca tgtcaaacgg acacatcgaa aaggccttcg aaacagcaga ccgtcacggg
    12001 ctccacacat ccgcatttgt tatgataggg ttacccgatg aaaagaaaat cgacctcatg
    12061 gaaacggtta aactccttgc taaaataaaa ccgggcaggt ttcgctggtc tctcttcttt
    12121 ccttttatag gaaccagggc ttacacaata gccaaggatc tgggggccat agactttgag
    12181 aagatggacc gccttgacaa cttcactgat gaaacatgca tgaatctcgg accggatgag
    12241 gatcttctta tagacaaact gaaaacacta ttttgtcttt ttgtaaatgg gtacgctgac
    12301 attgatggca aagggaaata tgctggtctt ttaagagaag tcgaggatgc agatgaggct
    12361 gcttggcagc gaaataaaac caagttttcc gaaaggctga agactcttga tagagaaatg
    12421 gcaaaccatg gaacaataca ctacacgata aaatataatc ggtttatggg tgtaagaagt
    12481 gactggcagg acgatagtct atcttcctaa ctggctcgga gacatggtaa tggcaacacc
    12541 attcctccat tccctcaggg cccacgttaa ggaagagctg tgggctattg gaaagaacaa
    12601 agcgttgcaa ctatataatg gtaatagtct ctttgaccgt ttcatccctt atgatggcaa
    12661 gggggtggtc ccgtttcttg atacggtaag ttttatcaga gccttaaact ttagggatgg
    12721 cgttatcctg ccgcactcat ttcgctctgc cctcttgttt tttgccgggg atgtccaaca
    12781 gaggataggt tatgcgcgca accaaagagg atttatgatt accctcaaag tagaggaggt
    12841 aacaaggcct gagccaacag ttgaacacta tttaaaaatc attgacgcta tgggtggtac
    12901 gagggttttg acaacgccct tgctcgaggt taccgaggag gaggacaaaa ggttcgacga
    12961 gaagtatgtg gacttgaatg ctccatatgt agtatttatc actggagctc agtatggacc
    13021 tgcgaaacgc tggcccgacc actattttgc tgaacttgca aataagattg cacgagaact
    13081 ggatatgaat gtttatcttc tgccaggttt cggtgaggaa gaacttgcac agaagattta
    13141 tcaaaacgta gaacacaaga agcgtgtttt tgtgaaagtc atggatatca tggaacttaa
    13201 agtgtgtctt tccagagcag cggctgtcgt gacgaatgat actggacccc gccatatctc
    13261 agcagctctg tctgtaccca cagttgtcct ccttgggcct atggatgaac aatatacgct
    13321 ctatcccagt tcctgtacct atgtattttc aaaggatgtg gcatgcagac catgcaacaa
    13381 aaagcgatgc gacaagaacc atgtgtgcct tacgagtatt agcccgaatg acgtgtttca
    13441 gaaactggag gaaatccttg aagtccgacc gagtaaagct cattgaacgt ttcgatggaa
    13501 ggaagatatg cgttgtcggt gatatcatag cggatatcta tatttttgga agaccctata
    13561 ggctttcacg ggaagcgccg gttgtagtag ttcaacatga agaagagcgg atttatccag
    13621 gcagtgcagg caacacaatt aataatcttc ttgcattagg ggctcacgta tttccccttg
    13681 gctttattgg aagcgatagt actggagacc agcttattga ttttttttta cgtttcaaca
    13741 aggtcgagat ggacggcttt attcgtcata atggtgagac tataaccaaa acccggatac
    13801 ttgctggaga cacacataca tcaaaacagc aagttatcag gatagaccgg gacagcaaca
    13861 agttacctac taagtctcaa aaaaaccttc tctacaaaaa gttaaaagag atagggccgt
    13921 ccatggatgc ttttatcgtc tctgattatg gacatggtac agtcgacgag actatcatcg
    13981 attatatgca acatgtggca aaaaaaacca ttgtcgttgg agatgcccgc tacagactga
    14041 gggactttaa gggttttacc ctgataacac ccaatgaatc tgaagcatac cagctttgca
    14101 gcacagattt tgataaggat attgaagagg ttggaagaag aatcttaaaa atcatggatg
    14161 taactgcctt actcataaca agaggaaata agggtatgag tcttttccta aaggaagggt
    14221 ctgtcaatca catacctatt tcaggaaacg atgacgtgac agatgtgaca ggtgcagggg
    14281 atacggtctg tgcggctgtc acacttgcac tagcaagtgg tgcagatttc ctgaccgctg
    14341 cattgatagc aaattatgca gctggcgttg tagtaatgaa gcgtggaact gccacattaa
    14401 ctcaagaaga attgagggcg gcaatgcaag cttatgggac aaatcgttaa aaaacttgac
    14461 gagctaaaaa gtattgtaga aaaagaaaag aaccagggca agaagatcgt ctttggtaat
    14521 ggttgcttcg atcttcttca tgttggtcac gtacgttacc tgaagggagc gaaggcattg
    14581 ggggatgtgc ttattgtggc tgtcaatgac gattcatcgg tgatgggcct gggtaagaga
    14641 aaacgtgtgg ttacaccggc gcctgaacgg gccgaaatta tcgctgcctt agaaggtgtt
    14701 gactacgtaa tccttttcag tgatccaaac gtggagaatc tattgcgcac acttcaaccc
    14761 catatccatg caaaaggaac tgactacacc gaggaaagtg ttcctgaggg caggattgtg
    14821 cgcgattatg gaggtcgtgt tgccatagta ggtgacccaa aagaccattc aacgagggac
    14881 ctgatcaagg ctatcaagga catggattaa attaatggaa tgacgatggg gggaagaacg
    14941 tggatcaacc agtggcaagc aatcagcaaa agcaaacgct gaacagcgaa cagctaacgc
    15001 caagcgacgg gctgccttta tcggtctaca tgataaccta taataatggt gtaaccatcg
    15061 agagggccct gcagagtgtt gccgattggg cagatgaagt gatagttgtg gactcttaca
    15121 gtacagatgg ggctcaagat attattccac ggtatacaga aaagctttac cagtttgaaa
    15181 ccaaagacct gcgggagaag tatcaatatg cccatgatca gtgcatcaac ccctggacac
    15241 tctttatcga tgctgacgaa tggctcatgg atgacataaa aaaagagatt tccaccataa
    15301 ttcgtggcac accctcctgc gatggataca ttgcaagtag aagaaacatg tatcttggca
    15361 gggaaataaa acacggcggt tggtatcctg acagagaaat ccgtctgtat cggagagaaa
    15421 aaggcagatg ggaagggggg ctacacgcaa aggttactgt tgacggcaca gttggtaccc
    15481 tcaaacattt ctatatgcat acaccctatg cagacattgc tcaccagatc agaacaattg
    15541 accgatactc cgaggcattt gcagaggatc ttcgctcctc gggtagacgt tttcacttag
    15601 taaatttgat cacacgtcct gtttaccggt ttttcaggga ttatatcctg aaacgaggtt
    15661 ttctggacgg aacgcctgga tttatcatcg ttgtctccac catgtattat gtctttatga
    15721 agtacgccaa gctttgggag atagagatga aggagaaaaa gcagttcaga gaccggtttt
    15781 agaagttcga agtaaaggct tcaaaaacct tacgtggtaa tgaactggac ggagagaaga
    15841 gaatatgaac actttcaaag ataaagaggt tctcataaca ggtggtcttg gcttcattgg
    15901 aagcaacctt accatagatc ttgtcgaact gggggcccat gtaacgatag tcgacaacat
    15961 gctcccgaga caaggaagta accttttcaa cattaaagag gttgaggaca gggtgagggt
    16021 caacatctct gatatccgaa accagctctc catgaatcac cttgtaaagg gaaaagacta
    16081 tatctttcac ctagccggtc aggttaacca tgttgacagt atgagaaatc cccttcaaga
    16141 ccttgagata aactgtgtcg gcacgcttgt tctcctcgaa gcgctgcgtc acaacaacag
    16201 agatgcaaag gtcatatttg cgggtacccg gggagaatat ggcgcaagcg tgaaactgcc
    16261 agttaacgaa gaccacccaa caaacccgaa agggctctat gcagtaacca acctggctgc
    16321 agaaaagatg gttctggtgt atgacgatat ctttggtatc aaaagtgtct gtctacggat
    16381 aaccaacacc tatggaccc
//
LOCUS       DAOOMC010000062        16323 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdus sp. isolate AMR_MDS_2685
            NODE_3756_length_16323_cov_6.240165, whole genome shotgun sequence.
ACCESSION   DAOOMC010000062 DAOOMC010000000
VERSION     DAOOMC010000062.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864760
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdus sp. (wastewater metagenome)
  ORGANISM  Syntrophorhabdus sp.
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae; Syntrophorhabdus.
REFERENCE   1  (bases 1 to 16323)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16323)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,729
            Genes (coding)                    :: 2,708
            CDSs (with protein)               :: 2,708
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 7 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16323
                     /organism="Syntrophorhabdus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3756_length_16323_cov_6.240165"
                     /isolate="AMR_MDS_2685"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2699756"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(581..970)
                     /locus_tag="PK125_07680"
     CDS             complement(581..970)
                     /locus_tag="PK125_07680"
                     /inference="COORDINATES: protein motif:HMM:NF014678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF192 domain-containing protein"
                     /protein_id="HPB38024.1"
                     /translation="MTNSETLIIYSDMGSETCRFDVEPAITHEEQARGLMFRKSLDDK
                     AGMFFIFQREELRHFWMKNTLFPLDLVFIDNNFLVVDIHKNAKPLDESIISSRKPARY
                     VLEIKAGKADKCRMRNGTKTKLLKNSF"
     gene            complement(1057..1713)
                     /locus_tag="PK125_07685"
     CDS             complement(1057..1713)
                     /locus_tag="PK125_07685"
                     /inference="COORDINATES: protein motif:HMM:NF008102.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HPB38025.1"
                     /translation="MELLKTLVDIFLHIDKHLSEIIQNYGLWTYLILFIIVFMETGLV
                     VTPFLPGDSLLFAAGAFAALGAFDIVWLFAILSVAAIGGDTVNYWIGSYIGPKIFHKE
                     KVRFLNKEYLDRTHQFYERYGGKTIIIARFVPIIRTFAPFVAGIGSMTYWKFISYNII
                     GGIAWILICTLAGYFFGNLPMIRENFSLVIFAIIIISILPGIIEFMRQKMLTAPANKD
                     "
     gene            1854..2705
                     /gene="htpX"
                     /locus_tag="PK125_07690"
     CDS             1854..2705
                     /gene="htpX"
                     /locus_tag="PK125_07690"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF002826.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metalloprotease HtpX"
                     /protein_id="HPB38026.1"
                     /translation="MNQTKTFFLMVVLTIIFVALGSMIGGRDGGMIAFFIALVMNFIS
                     YWFCDKIVLKMYRAQEVSEAEAPQLHAMVSALAQKASIPMPRVYIIENDSPNAFATGR
                     SPSKGVVAVTTGIMRILSREELEGVLAHEISHIKHRDILIQTVAATLAGAITMIANFA
                     RFTAFFGGGSNDDEGRGNIFSVIIFSMLAAFAAMLIQLAISRSREYMADDRGAQLSGN
                     PLYLAGALKKLTLGVARSPMNDANPSTAPLFIVNPFSAKGIITLFSTHPPIEERIKRL
                     EDMAYRR"
     gene            2782..3999
                     /locus_tag="PK125_07695"
     CDS             2782..3999
                     /locus_tag="PK125_07695"
                     /inference="COORDINATES: protein motif:HMM:NF013362.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HPB38027.1"
                     /translation="MDHFFSGERLPAEQKSLIHEIVSGVFRWKGYFDWVLRQYARKNV
                     KKHVRYLIWASLYQVAFMKKSSHHVVKESVEYAKAEHGINTAGFVNAVLRKYLREIET
                     RRISKPGVISQTGSLSPDIMAVVYSFPQWLVQRWRKRFGHSKAELLFSHLNKPPEFAL
                     RVIEHNITKCEAITKLENLGLMVKPGKFLESALYVNRLAPLIQDELLRNKLISIQDET
                     SQLAVHSLQLKPGSLVLDACSGLGTKASHIMDLATGIRLVALDNSLSRLSFADRRSSL
                     ILGDANFAPFKGEVFDAIILDAPCSSLGIIRKHPEIKWRRRENDINHFSHKQYAMLKS
                     VWDKLKVGGHLVYSVCSFEPEETIDVINKFKNIRKFALENPLPFLFNKEYFVSLPFET
                     GMDGFFIARLKKL"
     gene            3996..4712
                     /locus_tag="PK125_07700"
     CDS             3996..4712
                     /locus_tag="PK125_07700"
                     /inference="COORDINATES: protein motif:HMM:NF015734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PASTA domain-containing protein"
                     /protein_id="HPB38028.1"
                     /translation="MTWKKGLLYSLLPFFVFFVSVYLTIDLLLKSGGNVICPDVRGKT
                     VDEAREVAKKTGLPLVVLRYENRSDVPYNHITVQKPEANIPIRKGRIIHVIVSEGPKL
                     TDVPNLIGITLQEAEETLKEKNLGIGKIIHIPHIKEGKIIAQTPKGGEKILEKKEVTL
                     FIAQNESKYYFLPELKDLNLWDIQGEMDTKQIKYRITLLPTVMINDRTVQQESLRLSL
                     PSRTIFKRDEEILIYINSGG"
     gene            4717..5397
                     /gene="rpe"
                     /locus_tag="PK125_07705"
     CDS             4717..5397
                     /gene="rpe"
                     /locus_tag="PK125_07705"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: protein motif:HMM:NF004076.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HPB38029.1"
                     /translation="MKKTLIAPSILSCNFLKLEEEIKDVEKAGADLIHVDVMDGHFVP
                     NITIGPMFVRALKTITTTVLDVHLMIKNPQQYVSEFIEAGADIVTVHAEADVHLLRTI
                     DMIRSQGRKAGVSLNPSSSLNLIEYALCAIDLLLIMTVNPGFGGQQYITTMEPKITAA
                     RDMIKKTGRNIDLEVDGGIKATNVQRVVKAGANIVVMGTEIFHSGDYAAKIREVRKIV
                     SSQDYGTH"
     gene            5384..6040
                     /locus_tag="PK125_07710"
     CDS             5384..6040
                     /locus_tag="PK125_07710"
                     /inference="COORDINATES: protein motif:HMM:NF024067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3786 domain-containing protein"
                     /protein_id="HPB38030.1"
                     /translation="MEHIKQKVYKKVYHMACEELLNANIPQRCEAAGVFYKEGSNNSI
                     IAIPFFNESITVTVPGFTFKSSKNTNITLVTRIILLHYLIKASGAKIGKDMVPYEDIP
                     GLRHYLPVYQKRALKPLQAAFGFDRHAFLEAGLSLGAIQQEYGDASFTLHVLPRISIT
                     FILWEGDSEFPPSIKTLFDPTIPDYLPLEDIVVVSKLAATRILKEARRQHAFDASDYS
                     "
     gene            complement(6062..6811)
                     /locus_tag="PK125_07715"
     CDS             complement(6062..6811)
                     /locus_tag="PK125_07715"
                     /inference="COORDINATES: protein motif:HMM:NF020287.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine-N1)-methyltransferase"
                     /protein_id="HPB38031.1"
                     /translation="MNTIAENDYVLIVYREKRYFKKVEMGKSFHGTGGVINYSDLVGV
                     EYGVKRGSYEFFKPTLEDIIMYGLRRETQIVYPKEAYHICFKLNLRNGSKVCEVGGGS
                     GALTCIFSRMVGPEGSVVTYEKEERHYKNIRKNIERFTEWDNVDLHHGDIMDSDKRNF
                     DAAFIDVREPWLIIEKIRSLMKGSTYLGIIVPTTNQIVETLRALSEGFGDVEVLELML
                     RRYKTVPERVRPEDRMVAHTGFLIFARKLNG"
     gene            complement(7149..7403)
                     /locus_tag="PK125_07720"
     CDS             complement(7149..7403)
                     /locus_tag="PK125_07720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB38032.1"
                     /translation="MKSIQIVKKLCNRPQKKIFLVAAIENNAHALVFLTWYEEFLTFY
                     ADGILGMLTIMTGLALALKATAIRPLCCLAYFQCFPTICL"
     gene            7606..8253
                     /locus_tag="PK125_07725"
     CDS             7606..8253
                     /locus_tag="PK125_07725"
                     /inference="COORDINATES: protein motif:HMM:NF013844.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HPB38033.1"
                     /translation="MKKSTSIYTILSDLPEFIKDRKISLKGLIRDEKALYAEAMEGVK
                     KISKKKTIIKRPNTIIKIHPINPRNDPKHLLEEAISDRRKMNVTNMPEYMEGYAEGTN
                     PLALEKLKNGEFSVQKTLDLHGLSIENAKLSFEQFIIDSIKLGLTCVKVVHGRGLRSR
                     NVPVLKENLKTWIVRAMNRKWVTAFSSARMCDGGPGASYILLKKNPAKKKIHIFG"
     gene            8268..9068
                     /gene="proC"
                     /locus_tag="PK125_07730"
     CDS             8268..9068
                     /gene="proC"
                     /locus_tag="PK125_07730"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HPB38034.1"
                     /translation="MKKVGIIGMGNMGEAILKTLVKNIQHRTNIACAEAKPSRASFIV
                     KTYGIKCYKNPGDVVKKSDFVILAVKPQDSKTVLQSIAPFFNEKKVLISIMAGITTSN
                     ILSMIGKTIKIIRIMPNICVKVGEGLMGVSINNHVESVDVEDVKAMFSSMGQTIEVNE
                     ELMDSVTALGGSGPAFVLLFLEAMIDAGVKLGLPRDKSSAISSQVIKGTIKMLDMENL
                     HPTLMREMITSPGGTTIAGLATMEQQGFKGIVIRAIEDSCKRSKELSV"
     gene            9068..9718
                     /locus_tag="PK125_07735"
     CDS             9068..9718
                     /locus_tag="PK125_07735"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: protein motif:HMM:TIGR00080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HPB38035.1"
                     /translation="MFNEYTSKRQKMVETQLIPRGIKDPRVIEAMRKVPRHLFLDEAL
                     WPEAYEDHPVPIGEKQTISQPFIVAIMTEALQLKGREKILEVGTGSGYQAAILAELAD
                     QVYSIERLPSIAKRARKILDQLKYSNIVITIGDGTLGWKEHSPYDGIIVTAASPYAPN
                     TLLEQLTIGGRLIIPLGDEFTQDLTLYIKETKDNYNKESYGGCRFVKLIGEKGWKE"
     gene            9706..10581
                     /locus_tag="PK125_07740"
     CDS             9706..10581
                     /locus_tag="PK125_07740"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HPB38036.1"
                     /translation="MERIIGESQKSDFFADEEFKAEKLYMKDLRKLPVITMEEEKEYA
                     RRIAQGDHVARRKMIEANLRLVVKIARKYMHQGISHLDLFQEGNLGLIRAVEKFDPSR
                     NCRFSTYATWWIRQSIERAIANFSRTIRLPIHISSRVYQISKIISNFMEKNGKEPSSQ
                     EIAEISGLPLDFVTNLFSVVIKTYSLETIIDEDGKLTLEDVLVNTSAEEPLSAFEQTR
                     RVEEVASWLEVLGDDEKRVIILRYGLDGEEPQTLESIGKVFGVTRERIRQIEQKAINK
                     LRRIVKRRNIGTENI"
     gene            10562..12091
                     /gene="murJ"
                     /locus_tag="PK125_07745"
     CDS             10562..12091
                     /gene="murJ"
                     /locus_tag="PK125_07745"
                     /inference="COORDINATES: protein motif:HMM:TIGR01695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HPB38037.1"
                     /translation="MGQKISDIDSAHTSTSDIIKKGSVITIITMISRPLGYIREAIQA
                     YLFGATLLVDAFIVAFNFPELIQTLFFSGATSAFLVPVCVHYMKDHEEYSSIYSTFIN
                     ISIIVTVSISLLFLAFSHGIVSMIAPGFSSEAKQITKTLFIIMLPVIAFHTILSVIKA
                     FLNAKDHFAAPELSGIVWNIAFILSAFLLRHKLGIYSLAVGVTVGSLLQIAIQVPFLK
                     QFNIRYRMVFNLQHPALVEAKNLFTGALIATSIVPINSFVGRVIASYLPHGEVAALSY
                     AFRIFILPFSLFAVPIYTVMFTKLSKLFHSQDWRSINAHIDSSLVLIAITLVPCTVLL
                     CSVGDAVIRVLYERGAFTGNDTLMTNRALFGYSVGLIFYALSISFVRIFNSFHDMKTP
                     AIIGISSVIINAILASLLMVPFRNLGISLATSLVSFYNFVLLYIFLKKKTGYSPKKGT
                     IREISRSLICGIILLITIFVSRQLFSENLVVILFTCAALTLIIYGFAFKKYYYFFLKK
                     K"
     gene            12289..13182
                     /locus_tag="PK125_07750"
     CDS             12289..13182
                     /locus_tag="PK125_07750"
                     /inference="COORDINATES: protein motif:HMM:NF019874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cache domain-containing protein"
                     /protein_id="HPB38038.1"
                     /translation="MEKTKGRIFFSFFLTVIAGFFLLGSSTIEPVHREGRQLINLVRE
                     AAKLIEQKGESCFSEFKKKGGKWLHGDTYVFVIDTKGFVFVNPSRPELEGKEQISLKD
                     LMGKPFIRSFINETTGYPFKNEGWTHYVWYKPGEEMAMWKTSYVKLVKAPSGKDFIVG
                     SGLYDIRMEKAFVVDVVDEAADLIKKKGKQAFAQIRDPLGDFNYLTTYVFVIDSKGMD
                     LVNPVFPGFEGQNVLDLKDSEGKYFIKDMIRALESTDTVWIEYMWPKPRHAVPTKKST
                     YVRKVLYGEEVFYVGSGVYLD"
     gene            13338..14888
                     /locus_tag="PK125_07755"
     CDS             13338..14888
                     /locus_tag="PK125_07755"
                     /inference="COORDINATES: protein motif:HMM:NF012686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HPB38039.1"
                     /translation="MNTQFLPVPEITWTMAITSSIGIAVLLGVSILLGILGKREWKSF
                     DEYLVGKRDMGPLVTGCALSASYLSGWAFCGSTGISYSVGFSGMWFAGMWSLIGIIPC
                     IWVASAKTRSFAVQLSATTVPELMGRRYESKMLQTVISFCMLFFLFIYSVGQLKAAGG
                     VWYAVTGFPPVLCLFLGVFIAWIYMVLGGYVGTNWSMAFQGAFLGIVGAIFGIWALLS
                     SGGFLEISSKLWEQDPKLLQLIRPDLPSVGPTEFFSSWAGFFAAPLIFFTMAIGFPHN
                     LGRFLGMKKMNKKEFWLLILIVWFITGIPVMLASSTNGLITRTLFGPQLFNIEPWKAD
                     LAAPLLSYAVGGIPLQTLYVMGLFAATLSTLAGMVMIMSANVTRDIITLWRPQTTDKT
                     LIRTSYFLIALFLFLPFYWTWKRPPELLSVFMGLAAMGLGAIFFFVTAMSFYWKRATK
                     WGAFWTVIYGTVASLAGGYFVFTKKVLGMATMEFILLAGCFILFFVVSLITKPPSREL
                     IDKLFAKK"
     gene            15014..>16323
                     /locus_tag="PK125_07760"
     CDS             15014..>16323
                     /locus_tag="PK125_07760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB38040.1"
                     /translation="MDVMIHAPTMEFHVSRQVRDFYDFSESLFSTTGNVIFINFHSAR
                     IFVQKMNDKRDILNYPEQAVKTGHIVTMGLIDEILHHVVFLYRQEIDSEIMGKALVFL
                     YETMGEEKVDNALRAFIDEFPPLTVYRQGLSVEQYAAGITDNVPNRYIVLEEMLLLWL
                     ANMNPAFSQFRELFDDSNLKKNTVYLEIITYLKKFFEKNPFFGPYKQPLIDMLRSPAV
                     EVPHSLPGQLEYIRQHWGFLLSHYLSKLLASLDLVKEEQKLTFLGPGPAEVYKYKGLE
                     LEPEQFTPDREWMPRLILIAKNIYVWLDQLTKAYGRPITKLSEIPVEELLMLRDRGFS
                     GLWLIGLWERSPASQRIKQLCGNPEAVPSAYSLYDYQIAADLGGEEAYQYLRDTAWNY
                     GIRLASDMVPNHVGIFSRWVIEHPDWFISLNHNPFPWYTYGGVNL"
BASE COUNT         4873 a         3343 c         3288 g         4819 t
ORIGIN      
        1 actctaccaa cctgagttaa cggcccctga aggttataag aatatgaaat attatggata
       61 ttgtcaagcc taatactgct atttttgtcg atcctgtgga gatcaatttc cttcatcatt
      121 ccagtcctta aacacaagca aatgctaaaa gcaatttcat tattttagag atgacctaac
      181 aacaggactt gcggataggg ctctttttat cctcacctaa cccactgaaa aggttgattt
      241 atagagaccg ctgtagagtg tgcagtggat attcattttt ggtcatccga ctgaacgctc
      301 tcggaaatca ttacagtttt aagttcacaa aaaattcact acaatcgatg taaagtctaa
      361 taaaatcgat gagcacatgt gtcacttcct caacttgttc acaggttttc cacagttttt
      421 gttaaaaact atggaatgct gcaaaggcta ttttgcgaaa aagagaatga gcagacgtgt
      481 gtatgtataa acgcctgaat gccagaaaga agatacatga tttaatacaa tgcaaaaaga
      541 aaaaacctga tcaacaagaa caacgcatct agataacagc ttagaacgaa ttctttaaaa
      601 gttttgtttt tgtcccattt ctcatccggc acttatcagc cttaccggct ttaatttcaa
      661 ggacataccg agctggtttc cgagaagaga ttatgctctc atcgagaggt tttgcatttt
      721 tatgaatgtc tacaaccagg aagttgttat caatgaagac caagtcgaga ggaaagagcg
      781 tgttcttcat ccagaaatgt ctcaactcct cccgttggaa gataaaaaac atccctgctt
      841 tatcatccag tgatttccgg aacattagtc cccttgcctg ctcttcatga gttatcgcag
      901 gctcaacatc gaatcggcat gtttccgatc ccatgtcgga atagattatc agggtttcac
      961 tgttggtcac gcatggcaca aaaaaacaaa tagttatgat aatgaactgt aaagaaatcg
     1021 atatcttggt cacttgctat caaaaacctt taatttttaa tctttatttg ccggtgcagt
     1081 taacatcttt tgacgcataa attcgataat acccggtaat attgatataa taattatggc
     1141 aaaaataacg agggaaaaat tctctctaat catgggcagg ttcccaaaaa aatatccggc
     1201 aagggtgcag ataagtatcc aggcaatccc accaatgata ttatagctga taaatttcca
     1261 gtatgtcatg ctgccaattc ctgcaacaaa gggggcaaac gttctgataa tagggacaaa
     1321 acgagcaatg atgatcgtct tgccgccata tctttcatag aactgatgtg ttctgtccag
     1381 atattcttta ttgagaaaac gcactttttc tttgtgaaaa atcttgggcc ctatgtacga
     1441 tcctatccag taattaaccg tgtcacctcc aatggcagca acggaaagaa tcgcaaaaag
     1501 ccatactata tcaaaggcac ctaacgcagc aaatgccccg gctgcaaaaa gaagggaatc
     1561 acctggtaaa aagggagtta caaccagacc tgtttccata aagactataa taaacagtat
     1621 cagataggtc cagagaccgt agttctgtat gatttcgctt aggtgcttgt caatgtgaag
     1681 aaagatgtcg acaagagtct tgagcaattc catgcggtat actacactga tcgatagcat
     1741 gagtcaacaa acttaaggac taagttgtct gacttatata gtgcctttaa aaaacaaaac
     1801 aaaagaagta tactatgttt gatttaaaac ctaacaattt ttatggtgga caaatgaatc
     1861 agacaaagac attctttctg atggttgtac ttacaatcat ctttgttgct ttaggaagca
     1921 tgattggcgg acgagacgga ggcatgattg cctttttcat agctcttgtt atgaatttta
     1981 ttagctattg gttttgtgac aagattgtcc tcaaaatgta tagggctcaa gaagtatctg
     2041 aggctgaagc cccgcagctt catgcgatgg tatctgcgct tgcacaaaag gcgtccattc
     2101 ccatgcccag agtttacata attgaaaacg acagtcccaa cgcattcgca acaggaagaa
     2161 gcccatcaaa gggcgttgtt gcagtcacca ccggtattat gagaatacta agtagagaag
     2221 agctagaagg cgttcttgcc cacgagattt cccatattaa gcaccgagac atcctaatcc
     2281 aaacagttgc ggcaaccctc gcaggcgcaa tcaccatgat agcgaacttt gctcgtttta
     2341 cagcattttt tggtggagga agcaatgacg acgagggaag aggaaacatt tttagcgtta
     2401 ttattttttc aatgcttgct gcttttgctg caatgttgat acagttagca atttccaggt
     2461 cacgggaata tatggctgat gatcgtggag cacagctttc aggaaacccc ctctacctcg
     2521 ccggtgccct taaaaaactg accttggggg ttgcccgctc tcctatgaat gatgccaatc
     2581 cctcaacagc accgcttttt attgttaatc cgttcagcgc aaaagggatt attacccttt
     2641 tcagtaccca tcctccaata gaagaaagga taaaacggtt ggaggacatg gcttatagac
     2701 gataaatcct ggttcactct gcgttcactt atagttacaa tcattgaaaa ggttgaatcc
     2761 ggctggcttc ttgacgaaac gatagaccac ttcttctctg gtgagcgcct gccggctgag
     2821 cagaaaagtc tcattcatga gattgtttcg ggtgtattcc gatggaaggg gtattttgat
     2881 tgggtactca gacaatatgc cagaaaaaat gtgaagaaac atgttagata tctcatttgg
     2941 gcttctctgt accaggttgc cttcatgaaa aaatcctctc accatgtagt aaaagagtct
     3001 gttgaatatg caaaggctga acacggtatc aatactgccg gttttgtcaa tgctgtttta
     3061 agaaaatacc tcagagaaat cgagacaagg cgtatttcta aaccaggcgt tatcagccaa
     3121 acaggatccc tgtcacctga tataatggct gtagtctact catttcccca atggcttgta
     3181 caacgatgga ggaaacgttt tggacattct aaagccgaat tacttttctc tcaccttaat
     3241 aaaccgcctg agttcgctct tagagttatt gagcataaca ttacaaaatg tgaggcgatc
     3301 acgaaacttg aaaatttggg tctcatggtt aaacctggga aatttcttga atccgccctc
     3361 tatgtcaata ggcttgcccc tttaatccag gacgagcttc tcagaaacaa attaatatct
     3421 atccaggatg aaacgtcaca gcttgccgtc cattcgcttc agttaaaacc cggtagtctt
     3481 gttttggacg catgctctgg actgggtacc aaggcttcac atattatgga tcttgccact
     3541 ggtatccgtc tggttgcctt ggataatagt ttaagccgtt taagctttgc tgatcgtcgc
     3601 tccagcctga tactgggaga tgcaaatttt gcccctttta aaggtgaggt ttttgatgcc
     3661 atcatccttg atgcgccatg ttcttccctc ggcataatac gaaaacaccc tgagattaaa
     3721 tggagaaggc gcgaaaacga tattaatcat tttagtcaca aacaatatgc catgcttaag
     3781 tcggtttggg ataaactcaa agttggtggg catctcgttt acagcgtctg ctctttcgaa
     3841 ccggaagaga caatcgatgt tattaacaaa tttaagaata tcaggaagtt tgcacttgaa
     3901 aatccccttc ccttcttgtt taataaggag tacttcgttt ccttaccttt tgaaaccgga
     3961 atggatggct tctttatagc aaggttgaaa aagttatgac atggaaaaaa gggttgctct
     4021 attctctcct tcccttcttt gttttttttg tatctgtcta tcttaccatt gatcttttac
     4081 taaaatctgg tggcaatgtc atctgtcctg atgtacgagg aaaaactgtt gacgaagccc
     4141 gggaggttgc aaaaaaaaca gggcttcccc tcgtagtatt aagatatgaa aacaggagcg
     4201 atgttcccta taaccatata accgtacaga aaccagaagc aaacatccct attagaaagg
     4261 gcaggattat ccatgtcata gtttctgagg gtcctaaatt aacggacgtg cccaacctta
     4321 ttggcataac ccttcaggag gccgaagaaa cgctaaaaga aaagaatctt gggataggaa
     4381 aaattattca tattcctcat attaaagaag gtaagattat agcacagaca ccaaagggtg
     4441 gagaaaaaat acttgaaaaa aaggaagtaa cgctttttat tgcgcaaaat gagtccaagt
     4501 attatttttt accggaattg aaagatttga atctctggga tatacaaggg gaaatggata
     4561 caaaacaaat taaatacagg atcaccctat taccaaccgt gatgattaat gacagaacgg
     4621 ttcagcaaga aagcctgaga ctttctctcc cctccaggac catatttaaa agagatgagg
     4681 aaattcttat atacataaac agtggaggct gaaacgatga aaaagacctt aatagctccc
     4741 tcaatccttt cgtgtaactt tctcaagctc gaagaggaga taaaagatgt tgaaaaagct
     4801 ggtgccgatc ttattcatgt tgatgttatg gatggccatt ttgttccaaa tattacgatc
     4861 gggcccatgt ttgtcagagc acttaagacc atcactacaa cagtacttga cgttcatctt
     4921 atgatcaaga atccacagca atacgtatct gaattcattg aggccggagc tgatattgtt
     4981 actgtacacg ctgaggctga cgtgcacctt ctaaggacca tcgatatgat acggtcacag
     5041 ggcaggaaag ctggtgtatc attgaatcct tcatcatcgc tcaatctcat tgaatatgca
     5101 ttatgcgcga ttgatcttct cctcattatg accgtgaatc ctggatttgg cggacaacag
     5161 tatattacaa ccatggaacc aaagataact gcagcacgtg acatgattaa aaaaacgggc
     5221 aggaatatcg atcttgaagt ggatggtggt atcaaggcaa caaatgttca gcgtgttgta
     5281 aaagccggtg caaatattgt tgtcatgggt acagaaatat ttcacagtgg agactatgca
     5341 gcaaaaatta gggaagtgag aaagatagta agctcccaag actatggaac acattaaaca
     5401 gaaggtttac aaaaaagtct accatatggc atgtgaagag cttctcaatg ccaatattcc
     5461 gcagcgttgc gaagcagcag gcgtattcta taaggaaggt tcaaacaatt caatcatagc
     5521 gatacctttt tttaacgaaa gcatcacagt caccgtccca ggattcacct tcaagagttc
     5581 gaagaatacc aacattactc tcgttacaag aataatcctt cttcattatc tgatcaaagc
     5641 atcaggagcc aaaattggga aggatatggt accttatgag gacattcctg gcctcagaca
     5701 ctaccttcct gtttatcaaa aaagagcttt aaaaccactc caggcagcat tcggatttga
     5761 ccgacatgca tttcttgagg ccggcctctc attaggtgca attcagcagg aatacggtga
     5821 tgcgtcgttt actttacatg tgctgccaag aatatctatc acatttattc tctgggaagg
     5881 agattcagag tttccaccat cgataaaaac gctttttgac ccaaccattc cggattactt
     5941 gcccctggaa gatattgttg ttgtctccaa actcgctgca acgcgtattt taaaagaagc
     6001 aagacggcaa catgctttcg atgcaagcga ctattcttaa aagagtacta tttcaaaaag
     6061 attaaccatt caattttctc gcaaatataa gaaaacctgt atgagcaacc attctgtctt
     6121 ctggcctcac ccgctcggga acagtcttat acctccgcaa catgagttcc agcacctcaa
     6181 catctccgaa accttcactg agtgcacgga gtgtttcaac aatctgattt gtggtgggca
     6241 ctataatccc taagtatgtg cttcctttca tgagactcct tatcttttca ataatcagcc
     6301 agggttcccg cacatcaatg aatgcagcat caaaatttct tttgtcagaa tccatgatat
     6361 caccgtgatg aagatcaaca ttatcccact cggtaaagcg ttctatattt ttgcgtatat
     6421 ttttatagtg cctctcttcc ttctcatagg ttacaacact gccttcaggc cctaccattc
     6481 tcgaaaaaat gcaggttaat gcaccgcttc ctcctccaac ctcacaaacc tttgaaccat
     6541 ttctgagatt gagcttgaag caaatgtgat aggcttcctt cgggtaaaca atctgggttt
     6601 cccttctaag cccatacatg attatgtctt ccaatgtggg cttgaaaaac tcgtaacttc
     6661 ctcgtttcac accatattca acacccacaa gatcagaata atttattaca ccccctgtac
     6721 catgaaacga tttccccatc tcaacttttt tgaaatacct cttttccctg taaacaatta
     6781 atacgtaatc gttttccgca attgtgttca attatatcct ctccgcttca gtaaattccc
     6841 acagaagagt ccgtggaact ggtatatctt ttccttacgt gtggctagtg tcactgtttt
     6901 gccataataa taaatgatat ggcagttatc aatttacaac cttatgttgt ataatttttt
     6961 caaattctac aactactttt ggcaattcca taggaataac accgccaaca ttaaatgtgt
     7021 ttgtcatcga aacaattgtg agaagtttga tcatgacgtc tatgaagggt gtgtcttcat
     7081 gttgacagca taaaactctt ctcttccatg ctgtatatcc catttaagaa acataatttt
     7141 aatctttctc acaagcagat tgtaggaaaa cactgaaaat atgcaaggca acacaggggt
     7201 cggatcgcag ttgccttcag cgccagcgca agacctgtca ttattgtcaa catacccagt
     7261 atgccatctg cataaaacgt tagaaattcc tcgtaccatg tcaagaagac cagtgcatgg
     7321 gcattgtttt caattgctgc cactaaaaaa atcttttttt gagggcggtt gcaaagcttt
     7381 ttcactatct gaatactctt caagatttac cttttttata aaatatacct ctttgaaaat
     7441 acgataagtg aggttattgg cataaaaata atacgtccga ttatgccaaa tctattaaat
     7501 aaccatgttc ctttcctgtt aatctattat aaatagctat tattgtcaaa acactgccat
     7561 tcatctactt gaacttattt cttctttaga gtaaaactaa caaacatgaa aaaatctact
     7621 tccatttata caatcctctc tgatttgcca gaatttatta aagatagaaa aatatcactt
     7681 aagggtttga ttcgggatga aaaagccctt tacgctgaag ctatggaggg tgttaagaaa
     7741 atctccaaaa aaaagactat tataaaacgc cccaacacaa ttataaaaat tcatcccatt
     7801 aatccaagaa atgatcctaa acatttgctg gaagaagcga tatctgacag acgaaagatg
     7861 aacgtgacaa acatgccgga gtatatggag ggttacgctg aaggaacaaa ccctctggct
     7921 ctagaaaaat taaagaacgg agaattctct gttcaaaaaa ccctcgatct ccatggcctt
     7981 tcgatagaaa atgcgaaatt gtctttcgag cagttcatca ttgattctat aaaattaggc
     8041 ctcacctgcg ttaaagtggt tcatggcaga ggtttaagat ccagaaatgt tccagtttta
     8101 aaggaaaatt tgaaaacatg gattgtacgt gccatgaaca gaaaatgggt tacagctttt
     8161 tccagtgccc gcatgtgcga cggaggtcca ggagcatctt acatactcct taagaaaaac
     8221 ccggctaaaa aaaagattca catctttggt taagaaaagg gggtcaaatg aaaaaagtag
     8281 ggataatcgg catgggcaat atgggtgaag caattttgaa gactcttgtg aaaaacatcc
     8341 agcacagaac gaacatagct tgtgccgagg caaaaccatc ccgggcaagt tttattgtaa
     8401 aaacatatgg gataaagtgt tataaaaatc ccggagatgt tgttaaaaaa agtgattttg
     8461 tgatccttgc cgtaaaacca caggattcaa aaacagttct gcaatcaata gctccttttt
     8521 ttaatgagaa gaaggttctt attagcatta tggcaggaat tacaacatca aatatattgt
     8581 caatgatagg taaaaccata aagatcatta ggattatgcc aaacatctgc gtgaaagttg
     8641 gtgaaggatt aatgggcgtt tctataaaca accatgtgga aagcgttgac gttgaagatg
     8701 taaaggcaat gttttcatct atgggacaaa ccattgaagt caatgaagaa ttaatggatt
     8761 ctgttacagc attgggtggt agcggcccag catttgttct gttatttctt gaagcaatga
     8821 tcgatgctgg tgttaagtta ggcctcccgc gagataaatc ttctgctatc tcatctcagg
     8881 ttattaaggg aacgataaaa atgcttgata tggaaaatct gcatcctact ttaatgcgtg
     8941 aaatgatcac atctcccggc ggtaccacta tagcaggcct tgcaactatg gaacagcagg
     9001 gctttaaggg catcgtgatt cgtgctatcg aagactcttg taaaagatca aaggagctct
     9061 cggtgtaatg tttaacgagt atacttcgaa aagacaaaaa atggtggaaa cgcaactgat
     9121 accacgagga attaaagacc ctcgtgtcat tgaggccatg agaaaggttc caaggcatct
     9181 ttttcttgat gaggccctat ggccagaggc atatgaagat cacccggtgc ctattggcga
     9241 gaaacagacc atttctcagc cttttattgt tgctatcatg actgaggcat tacagttgaa
     9301 agggagagag aaaatccttg aagttggaac agggtcaggt tatcaggcgg cgattctcgc
     9361 tgaacttgct gatcaggtct attcaatcga aagactccct tctatagcaa aaagggcgag
     9421 gaaaatactt gatcaactga aatattcaaa cattgtcatc acaataggtg atggtaccct
     9481 tggctggaaa gaacatagtc cctatgatgg cataatagtt actgcagcat ctccatatgc
     9541 tccaaataca cttcttgaac aattaacaat tggtggacga ctgataatcc ctttaggcga
     9601 tgaattcacc caagatttaa cgctatacat aaaagaaacc aaggataatt ataacaagga
     9661 gagttatggt ggttgtagat ttgtgaagtt aatcggagaa aagggttgga aagaataatt
     9721 ggagagagtc aaaaatctga tttctttgca gatgaagaat tcaaggctga aaagctttat
     9781 atgaaggact tgagaaaact tcccgttatc acgatggagg aggaaaagga atatgcccgc
     9841 cgcatcgcac aaggagacca tgttgcacgt agaaaaatga ttgaggcaaa cctcagactg
     9901 gttgtcaaaa ttgccaggaa atacatgcat cagggtattt cacatcttga cttgtttcag
     9961 gaaggaaatc ttggtctgat tcgtgcagtt gaaaaattcg atccttccag aaactgcaga
    10021 ttctcgacat atgcaacgtg gtggatcagg cagtccattg agagagcaat agccaatttt
    10081 tcccgtacta tccgacttcc catacacatt tcatcaagag tgtaccaaat atcaaagatt
    10141 atcagcaatt ttatggaaaa aaatggtaaa gagccatcat ctcaagagat agctgaaatc
    10201 tctgggcttc cactcgattt tgtcacaaat cttttttctg tggtaataaa gacatattcc
    10261 ctcgaaacca tcatagatga agatggtaaa ctaacccttg aagatgtgct tgtaaacact
    10321 tcagctgaag aaccactttc tgcgtttgaa cagacaagaa gggttgaaga ggtagcatcc
    10381 tggcttgaag ttcttggtga tgacgagaaa cgggttataa tcctcaggta tggcttagat
    10441 ggcgaagaac cccaaacact tgaatcaatc gggaaagtat ttggtgttac cagagagcga
    10501 atccgtcaga ttgaacaaaa agcaatcaat aaacttcgtc gcattgtgaa aagaaggaat
    10561 attgggacag aaaatatctg atatagattc ggcacacacc tcaacctctg atatcataaa
    10621 aaaaggctcg gtcatcacaa ttatcactat gatcagcagg cctcttggat atattcgaga
    10681 ggccatccag gcgtatcttt tcggtgcaac actcctcgta gacgcattca ttgttgcctt
    10741 taattttcca gagctgattc agaccctttt tttttcagga gcaacaagcg catttctggt
    10801 cccggtgtgt gttcattata tgaaagatca tgaggagtac tcttcaatat attccacttt
    10861 tatcaacatt tctattattg taactgtttc gatttcgcta ttatttcttg ccttcagtca
    10921 tggtattgtc agcatgattg ccccaggttt cagttctgag gcaaaacaga taacaaaaac
    10981 attatttatt attatgctcc ctgtaatagc tttccatact attctttccg taataaaggc
    11041 ttttcttaat gccaaagacc attttgctgc gcctgagtta tccggtattg tgtggaacat
    11101 agcattcatt ctttctgcct tcctgcttcg tcacaagctt ggtatctaca gccttgccgt
    11161 tggtgttact gttggatctt tgctccagat tgccatacaa gtaccttttc tgaaacaatt
    11221 caatattcgg tatagaatgg tgtttaactt acagcaccct gcccttgttg aggcaaaaaa
    11281 tctattcaca ggcgctctga tagcaacgtc cattgtacca ataaacagtt ttgtaggtcg
    11341 tgtgattgcg tcatatcttc cccatggtga agtagccgct ctctcctatg cattcagaat
    11401 tttcattctt ccattcagcc tctttgctgt gcccatttat acagtcatgt ttacaaagct
    11461 atcaaaactt tttcacagtc aagactggcg aagcataaat gctcacatag acagttcact
    11521 ggtactcatt gccattaccc tcgtaccatg tactgtcctg ctctgttctg tcggtgatgc
    11581 tgttatacga gttctttatg aacggggcgc ctttacagga aatgacacgc tcatgactaa
    11641 cagagcattg tttgggtaca gtgtcggttt aattttctat gccctttcta tctcttttgt
    11701 acgaatattc aactcttttc atgacatgaa aacccctgca ataataggca tttcatcggt
    11761 gataatcaat gctattctgg cttcgctcct catggtcccg tttcgcaatc tcggtatttc
    11821 cctggcaacc tctttagttt ctttctataa cttcgtgttg ctctatattt ttttgaagaa
    11881 aaaaactgga tattctccaa aaaaaggtac aattcgagag atttcacgct ccctcatatg
    11941 cggtattatc ttgttaataa cgatctttgt ttcacgacaa cttttttcag aaaatttggt
    12001 tgtaatatta ttcacgtgtg cagctctaac actgattatt tatggttttg cctttaaaaa
    12061 atattactat ttttttctaa agaaaaaata acttcccatt tattattttt tcgtaaaatt
    12121 aaacttggct tgtaagtgct tgaattatta tatcttataa atcttaataa aaaaaagtat
    12181 tgatattttg tttccgttgt atattaaatt tagaataaat catgtgaaca taatcacatg
    12241 atatccaaca tctgaaatcg aacaacaaaa tatgtgaggg ggtagctaat ggaaaagaca
    12301 aagggccgca ttttcttctc atttttcttg accgtaatag ctggtttttt tcttttgggt
    12361 tcaagtacta ttgaacctgt tcacagggag ggaaggcaat tgatcaactt ggtccgtgag
    12421 gcagcaaaac tcatagaaca aaaaggtgag tcatgctttt ctgagtttaa gaaaaaagga
    12481 ggcaaatggc tacacggtga tacgtatgtt tttgtaatcg ataccaaggg atttgtattt
    12541 gtgaatccat caaggcctga actagagggg aaagaacaga tttctttgaa agacctcatg
    12601 ggtaaaccgt tcatcagatc ctttatcaac gagacaacag ggtatccatt taaaaatgaa
    12661 ggttggactc attatgtctg gtacaagcca ggtgaggaaa tggcaatgtg gaaaacttca
    12721 tacgtcaagc ttgtgaaggc accctcaggc aaggatttta ttgttggtag tgggctttat
    12781 gatattagga tggagaaagc gtttgttgtt gatgttgtgg atgaagctgc tgatcttatc
    12841 aagaagaaag gaaaacaagc atttgcccaa atcagggatc cactcggtga ttttaattac
    12901 ctcaccactt atgtttttgt catagattcg aaaggtatgg atcttgtcaa tccagtattc
    12961 cccggatttg aaggacagaa cgttcttgac ctcaaagaca gtgagggcaa atactttata
    13021 aaagatatga taagagcatt ggaatcgaca gatacggttt ggattgaata catgtggcct
    13081 aagccacgcc acgctgtgcc tactaagaaa agcacatacg tgagaaaggt tctctatggg
    13141 gaagaagtat tttatgtagg gtccggcgtc tatcttgatt aaaattgttc acatcataat
    13201 tctatagagg aggttaatat tatgcaacct gataccacga cacttatgct tccctggtac
    13261 atctggtgga taatgattgt tggtttattt ctgatgacat ttattggcac atggctcata
    13321 acgaggaggt ggagcgaatg aatacccaat ttcttccagt tcctgaaata acatggacca
    13381 tggcaattac atcctccatt ggtattgctg tgctccttgg tgtctcaatc ctccttggca
    13441 tcctcggcaa aagagaatgg aagtcatttg acgaatacct tgtggggaag agagacatgg
    13501 ggcctctagt aacaggttgt gcactttctg catcttatct ttcaggttgg gcgttctgcg
    13561 ggtcaacagg catcagctat tccgttggat tctccggtat gtggttcgca ggcatgtgga
    13621 gcttaatagg aatcatccca tgtatctggg tggcatcagc aaaaacgcgg agtttcgctg
    13681 tccagctcag cgcaacaaca gtaccggagc ttatggggcg cagatacgaa tcaaaaatgc
    13741 tccagactgt cataagtttt tgtatgttat tcttcctctt catttattct gttggccaac
    13801 tgaaagctgc cggtggcgtc tggtacgctg tcactggctt tccgcctgtt ctttgcctgt
    13861 tccttggcgt ttttattgcc tggatttaca tggtccttgg tggatatgtg ggaacaaact
    13921 ggtcaatggc tttccagggt gcttttctcg gaatagtcgg cgcaatattc ggcatatggg
    13981 ctctactatc ttcaggtggc tttctcgaga tttcatcgaa attatgggaa caggacccga
    14041 aacttctcca gcttatcaga cctgatcttc caagtgtggg tccgaccgaa tttttctcaa
    14101 gctgggctgg tttttttgca gcacctttaa tcttttttac catggcaata ggcttcccgc
    14161 ataacctggg aaggtttctc gggatgaaaa aaatgaataa gaaagaattt tggctcctta
    14221 tcctgattgt ctggttcatc accgggatac ctgttatgct tgcttccagt acaaacggtt
    14281 taatcactcg gacactgttc gggccccaat tgtttaacat tgaaccatgg aaagcagacc
    14341 ttgcagcccc tcttctctca tatgcagtcg gcggtattcc tcttcaaact ctttacgtta
    14401 tgggcctgtt tgcagccacc ctctcaacac ttgccggaat ggtcatgatc atgagtgcaa
    14461 atgttaccag agacattatc accctatgga gacctcagac aactgataaa acgctgatca
    14521 gaacaagcta cttccttatt gcccttttcc tgttcctccc tttttactgg acatggaaaa
    14581 gaccccctga acttctttct gtatttatgg gccttgctgc catgggactc ggtgcaatct
    14641 tcttttttgt aaccgctatg tcgttttact ggaaacgggc aacaaagtgg ggcgccttct
    14701 ggacggtaat ctatggaact gtcgcaagtc tggcaggtgg ttatttcgtc tttacaaaaa
    14761 aggttttggg tatggcaaca atggagttta tccttctggc ggggtgcttt atccttttct
    14821 ttgtagtaag ccttataaca aaaccacctt cacgtgagct cattgataaa ctttttgcaa
    14881 aaaaataata aaaagggggt attcttatcc ccctttttta ttattttcca caggcatcat
    14941 ttccatgaac acattttagg tataaaatta ttttgttctg ccctcattga cccctatcct
    15001 tccatgtgat actatggatg ttatgattca tgcacccacc atggaatttc atgtctcacg
    15061 gcaggttcga gatttttacg attttagcga gagcctcttc tcaacaaccg gcaatgtcat
    15121 ttttattaat tttcatagcg ccagaatttt tgttcagaaa atgaacgata agagagatat
    15181 tcttaattat ccggaacagg ctgtgaaaac cggtcatatt gttacaatgg ggttgatcga
    15241 tgaaatcctg catcatgtag tttttctcta tcgccaagaa atcgacagtg aaataatggg
    15301 aaaggccctt gtttttctct atgagactat gggggaggaa aaagtagata atgcactcag
    15361 ggcctttata gatgaattcc ctcccctcac cgtctacagg cagggattga gcgtggaaca
    15421 atacgcagcg ggcataactg acaatgttcc taatagatac atagttcttg aggagatgct
    15481 cctcctttgg cttgcgaaca tgaaccctgc cttttctcaa tttcgagagc tcttcgatga
    15541 ttcgaatttg aaaaaaaata cggtatacct tgaaattatt acttatctca agaagttctt
    15601 tgagaaaaac cccttctttg gtccatacaa acagcctctc attgatatgc tccgaagccc
    15661 tgctgtagaa gttccccatt ctctgccggg ccaattggag tatatacgcc aacattgggg
    15721 ttttctttta agtcactatc tttccaagct acttgcgagc cttgatcttg taaaggaaga
    15781 acaaaaatta acgtttcttg gtcctggacc agcagaggtt tacaaatata aaggtcttga
    15841 acttgaacct gaacaattta ctcccgatcg tgagtggatg ccccggctta ttctcatagc
    15901 taaaaatatt tatgtctggc tcgaccagct tacaaaagcc tatggacgac caattacgaa
    15961 actcagtgag attccggtcg aagaacttct aatgttgaga gaccgtgggt tttctggctt
    16021 atggctcata ggcctatggg agcgaagccc tgcatcacaa agaataaagc aactctgcgg
    16081 gaatccagag gctgtccctt cagcttactc tttatatgac tatcagatcg cagcagatct
    16141 tggcggcgag gaagcttatc aatatctgag agatacggcc tggaattatg gaatacgcct
    16201 tgccagtgac atggtaccca atcatgtcgg tattttctcg cgatgggtca ttgaacatcc
    16261 tgactggttt atatccttaa accataatcc tttcccctgg tacacatatg ggggcgttaa
    16321 cct
//
LOCUS       DAOOMC010000063        16215 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdus sp. isolate AMR_MDS_2685
            NODE_3791_length_16215_cov_6.885644, whole genome shotgun sequence.
ACCESSION   DAOOMC010000063 DAOOMC010000000
VERSION     DAOOMC010000063.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864760
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdus sp. (wastewater metagenome)
  ORGANISM  Syntrophorhabdus sp.
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae; Syntrophorhabdus.
REFERENCE   1  (bases 1 to 16215)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16215)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,729
            Genes (coding)                    :: 2,708
            CDSs (with protein)               :: 2,708
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 7 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16215
                     /organism="Syntrophorhabdus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3791_length_16215_cov_6.885644"
                     /isolate="AMR_MDS_2685"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2699756"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..389
                     /locus_tag="PK125_07765"
     CDS             <1..389
                     /locus_tag="PK125_07765"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HPB38041.1"
                     /translation="KGIQAEISTHKEGILLKGDKEELTYLFYYLVQNSMEAAEPDNMR
                     VVIKSGLDPDSPSYVRIEIFNTGEPPKEKTEQLFSPFFSTKVKGTGFGLPIAQVVVRK
                     HLGRLDIKPMSGQGTNVIVSLPRATL"
     gene            complement(441..620)
                     /locus_tag="PK125_07770"
     CDS             complement(441..620)
                     /locus_tag="PK125_07770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB38042.1"
                     /translation="MKIPDEYICNLCGYSFLDGEVPENFCPNCGSSMVAKEMTYDEYL
                     DEEAFINMRIVNLTQ"
     gene            complement(874..1527)
                     /locus_tag="PK125_07775"
     CDS             complement(874..1527)
                     /locus_tag="PK125_07775"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HPB38043.1"
                     /translation="MKLFDDFYVYPWTSYQENNCNTIFIDGPVPTLIDPGHSHLFNHV
                     VEGMARDGKSADTIKLIVCTHSHPDHIEALDHFDNGVLRAISREEHGYLQEEGKELFL
                     MSGCQAPKKPFKLFLKEGQLTLGDKTLQVIPTPGHSPGSICLYWEKERVLISGDTAFY
                     MGVGRTDLPGGDMAALGQSIRRLAKFRIEYLIPGHGEMVRGDKPIKRNFELILGEFF"
     gene            complement(1536..2564)
                     /locus_tag="PK125_07780"
     CDS             complement(1536..2564)
                     /locus_tag="PK125_07780"
                     /inference="COORDINATES: protein motif:HMM:NF014610.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB38044.1"
                     /translation="MGEYASFADFTFSPMSSKAISLVSGGLDSAIATKLVLDQGIEVV
                     GLSFTSPFSSKKERSKGLQAIRTAKELGIRLILKEKGYEFIDVIRNPLYGYGKHMNPC
                     IDCRIFMLRKAKEVMAEEGAGFVITGEVLGQRPMSQRRSTIDLIEKKSGLTSLILRPL
                     SAKHFAPSMPEVEGIVNRELLLDIAGRGRAVQFGLVQKYGLSAFGDPGGGCLLCDALY
                     SMRLQDLLTHDKNFTMLDINLLNTGRHFRINRTTKLVLGRDKGENERLHSMWIPPYTL
                     VYPIDFHGPLGIIKGENEKGIIAIVANIISHYGRNQKSMIAVETNNGLICRHEVERAN
                     KDFERYKI"
     gene            complement(2572..3780)
                     /locus_tag="PK125_07785"
     CDS             complement(2572..3780)
                     /locus_tag="PK125_07785"
                     /inference="COORDINATES: protein motif:HMM:NF013958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine biosynthesis protein"
                     /protein_id="HPB38045.1"
                     /translation="MKSIDEINEKIKKGKVVVVTAEEMIGITKDKGTKKASEYVDVVT
                     TGTFGPMCSSGMFVNVGHSKPRIKIGGGKCYLNDVPAYCGIAAVDLYIGATASPDDDP
                     RNTVYPGAFKYGGGHVIEDFISGKNINLVATAYGTDCYPRKKIETFINKTNVNEAYLY
                     NPRNDYQNYNVAVNATDRVIHTYMGVLKPHLNSANYCSAGQLSPLLNDPKYRTVGIGT
                     RLFLGGGVGYVAWYGTQHNPDVQRSPEGIPRAGAGTLAVIGDAKQMSAEYIKGASFIG
                     YGATIFVGIGIPIPIIDEEMAYFTSRSDEDFWAQVVDYGNDYPNREPRSLGEVNYAQL
                     KSGAIRLNGKEIPTFPISSYSKAVSIATTLKEWIHSARFLLTNPVESLPGLDAGIKMN
                     TLEERILEYT"
     gene            complement(3823..4758)
                     /gene="cysK"
                     /locus_tag="PK125_07790"
     CDS             complement(3823..4758)
                     /gene="cysK"
                     /locus_tag="PK125_07790"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HPB38046.1"
                     /translation="MKIAENVTQLVGNTPLVKINKLARGLDAKIVAKLEYFNPCGSVK
                     DRIGVSMIEAAEKSGIINENSIIVEPTSGNTGIALAFVCAIKGYKLILTMPETMSGER
                     KAMLRAFGAELVLTPGAQGMKGAIDKAEDIIKNNPEAIMLQQFKNAANPEIHRKTTAE
                     EIWRDTDGAADAIVAGVGTGGTITGVSEVLKARKPTFQAIAVEPEASPVLSGGQPGAH
                     KIQGIGAGFVPDVLNTKVIDRIVKVSNEAAFRTARRMVHEEGIFCGISSGAAMWAALE
                     VAREPEYKNKMIVVIVPSLGDRYLSTELFLPYMKD"
     gene            complement(4942..5631)
                     /gene="tsaB"
                     /locus_tag="PK125_07795"
     CDS             complement(4942..5631)
                     /gene="tsaB"
                     /locus_tag="PK125_07795"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HPB38047.1"
                     /translation="MLNRLVLALDNSLDFLNIALAREHVLLEERHIKPHQPPSQIIPR
                     EVLNILISHGYGVDDLSLIITTLGPGSFTGIRVGLAFCKGLASGKSIPLIGVPTLDVL
                     VSSFSFLRGYFLCPLLDAKKGEVFAALYRVSHENIEQISDYQALKPAEVARMLKTPCL
                     CFGSGVKMCVDSLSSADGVYLIKEGFSRISAEALIRVGVKGALQGRPHDLKPIYGRRS
                     EAEIKFGVKVF"
     gene            complement(5621..6733)
                     /gene="rseP"
                     /locus_tag="PK125_07800"
     CDS             complement(5621..6733)
                     /gene="rseP"
                     /locus_tag="PK125_07800"
                     /inference="COORDINATES: protein motif:HMM:NF014244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HPB38048.1"
                     /translation="MNSIAFNIIPGIIVLGFLIFVHELGHFLMAKLTDVKVITFSLGF
                     GKKLWKRTWGETEYALSVFPLGGYVKLLGEGTEDEIRAEEAHRSYSNKPTIVKILIVF
                     FGPFFNILCAGLIFFVMLSSGAMLPASPSGPPAKVSGVIKDYPAAEVGILPGDTITAV
                     NGQKVAYFAEVQQFMAKTLDKPLRIEVKRQGQLLEFSVTPKEIETTNAAGAVVRRKVI
                     GIEAEKASGVQANNIFEAILLTPAYTYGVGEDTVIALWKIITGKMSRRNVGGPLSIFQ
                     AAGITAKQGTGSFAFFVAYISLSLAIINLLPIPILDGGHIIFSLIEVVIRRKISQKWI
                     ENAQKVGLALLIAIMVFAFYNDFDRFFGLSKFFNAK"
     gene            complement(6726..7877)
                     /gene="dxr"
                     /locus_tag="PK125_07805"
     CDS             complement(6726..7877)
                     /gene="dxr"
                     /locus_tag="PK125_07805"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: protein motif:HMM:TIGR00243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HPB38049.1"
                     /translation="MKKKILVLGSTGSIGQATLDVIHEQSHSFEVVGLACKDNISLLN
                     TQIQRFKPAYVSVYDNQLISHVKFPKKRLLQGMGGIKDLVGMDAHIVINAMPGSIGLE
                     PTIEALKNHKLLALANKESMVMAGRLVRKMLGQGNGAIIPVDSEHSALYQIMKTSGQD
                     DLKRLIITASGGPFRNYKKEKLKTVQPRDALNHPTWKMGNKITLDSATLMNKGLEVIE
                     ARWLFDIDPSHIDVLIHPESIIHGMVEYIDNSFIAYLAYPDMRIPISYALNEEKRRPL
                     PFAAINTNEMFKLTFYPPDTDRFPSLKIALEALADGDSSVIVMNAANEVAARAFIEGK
                     IRFTEIPVLIEKTLEQHHGSAVVEDIQAILEIHQWAEQVTEEKIQKEYE"
     gene            complement(7874..8692)
                     /locus_tag="PK125_07810"
     CDS             complement(7874..8692)
                     /locus_tag="PK125_07810"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: protein motif:HMM:NF013327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HPB38050.1"
                     /translation="MNDTKQRVLTTLCLAPIIVALFYLLPARWFFVLLALVACLSIYE
                     LITMSGIGERYLVLFLAILGFVPLYTQSIHIFLMWLLFSPVIFLITQFLQGENIKKDN
                     VNLNIMRGVSTLLLSEIFIVLPLFYLYLLKDVNSIFPLLLILALWASDIAAYFLGKTF
                     GKKLLVPRISPKKTYVGLLGAIIGPMIIMALSSEFMGTGIVESTIIGAFIGVLGQIGD
                     IFESAGKRVCDVKDSSSLLPGHGGMLDRLDSLIFTAPFLYHYFTGIKFLTGIRV"
     gene            complement(8693..9412)
                     /locus_tag="PK125_07815"
     CDS             complement(8693..9412)
                     /locus_tag="PK125_07815"
                     /inference="COORDINATES: protein motif:HMM:NF011405.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HPB38051.1"
                     /translation="MHCLDNEKLPRHVAIIMDGNGRWAKTKNLPRIEGHVTGIESVRD
                     IVTATRELGIPYLTLYAFSKENWVRPREEVQKLMGLLGVYLDKELPLLLEKGIRFKMI
                     GEMKDFPKKLQEKMYSVMKKTAKNKDLTLNLALSYSGRQEILHAIVSLFEDVKKGSVK
                     RINENVFKKYLYTGTIPDPDFLIRTSGEMRLSNFLLWQTAYTEIYVTKVLWPNFRKKH
                     YLSALKEYTKRERRFGRVEEY"
     gene            complement(9422..9979)
                     /gene="frr"
                     /locus_tag="PK125_07820"
     CDS             complement(9422..9979)
                     /gene="frr"
                     /locus_tag="PK125_07820"
                     /inference="COORDINATES: protein motif:HMM:TIGR00496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HPB38052.1"
                     /translation="MKNEIIDELEDKLNKSLTALKKDLSKLRTGVASISLLEDIKIVY
                     YNQPTPLNQVATIAVPDSRTITIQPWDNSAIGEIEKAIQKSDLGVNPASDGKIIRLSF
                     PKLTEERRKDLTKLGGKMLENAKVSIRNARREINDKLKKMEKDKLLSKDDAFKQQEDV
                     QKLTDKFIEIADKTYAEKEKEILEI"
     gene            complement(9985..10713)
                     /gene="pyrH"
                     /locus_tag="PK125_07825"
     CDS             complement(9985..10713)
                     /gene="pyrH"
                     /locus_tag="PK125_07825"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: protein motif:HMM:TIGR02075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HPB38053.1"
                     /translation="MEKPSKSSVAFKKILLKLSGEALMGGEGYGIDPATVKDVCLQIK
                     NVKDLGTQIGIVVGGGNIFRGMKAEKQGMERATADYMGMIATLLNALTLQETLEKIGV
                     PVNIQSAINIEKVAAPYDRREAIKNLVEGKVVIFACGTGNPYFTTDTAAALRAVEIGA
                     EALLKATKVDGVYDCDPVTHKNASLFQKITYMDVINKKLGVMDLTAVTLCMENSLPII
                     VFNLRGKDNMKKAIQGKRVGTMVR"
     gene            complement(10691..11296)
                     /gene="tsf"
                     /locus_tag="PK125_07830"
     CDS             complement(10691..11296)
                     /gene="tsf"
                     /locus_tag="PK125_07830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018286770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HPB38054.1"
                     /translation="MEISAELVKKLREKTGVGLMDCKEALKNAAGDMEKAIEYLREKG
                     LAKLQKRMGRTASEGVIASYIHTGAKVGTMVEINCETDFVANTKEFQEFAKDIAMQIT
                     ASNPLYVKREDVPEDAKEAEKNIYRKQAMESGKPEKIIDKIADGKMEKFYEEVCLIEQ
                     PFIKNQDITVKELLEELIVKMGEKIIVNRFVRFQLGETIEE"
     gene            complement(11311..12102)
                     /gene="rpsB"
                     /locus_tag="PK125_07835"
     CDS             complement(11311..12102)
                     /gene="rpsB"
                     /locus_tag="PK125_07835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HPB38055.1"
                     /translation="MSNLTIKQLLEAGVHFGHQTKRWNPKMKPYIFGARNGIYIIDLQ
                     KTLKMFKEAYNFVKDVASRNEYILFVGTKKQAQEAIQEEAKRAGSFYVNSRWLGGTMT
                     NFQTIEKSIDRLRKYEELKESDIFKALPKKEALGIDKEIAKLERNIGGIKGMDRLPGA
                     VYVVDPKKEYIAVSEAKKLGIPTIGIVDTNCDPDDIDFVIPGNDDAIRAIKLITMKVA
                     DAILEGKALYVEEFQGKEEPGEEPKPFVDESVLEERYEEYYEGSE"
     gene            12348..12479
                     /locus_tag="PK125_07840"
     CDS             12348..12479
                     /locus_tag="PK125_07840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB38056.1"
                     /translation="MASNTKKTESIRKRKRAAQGKKRKKVMSKMSTPAFPIHPDKTE"
     gene            12494..13636
                     /locus_tag="PK125_07845"
     CDS             12494..13636
                     /locus_tag="PK125_07845"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HPB38057.1"
                     /translation="MRYEIGPIRPPNEAYSLLVRFTRNCPWGKCAFCRSYKQRRFEKR
                     SVVEIKEDIDIIRKIKDEIVALSWQKGFGGEINDRLIDLIFSTPAYNEYFKGIAIWLY
                     FGGKNVFIQDANSLAMKPADFIEALTYLKQQFPSIDRVTSYARARTIAKRLTVDDLTR
                     MREAGLTRLHIGLESGSNSLLKYINKGVTKEEHIECGRKVKESGIELSEYVVLGLGGK
                     KWWKEHAEETADALNRIDPDFIRFRTLKVLRDMPLYEKVESGDFEILHEEEILIEEKH
                     LIERLEGINSTIKSDHILNLLEDVDGKLPEDKERILSVIDDYFSLNEEQRLVYRFGRR
                     AGMYRSINDLNDELTYLRIKKTIKEIEEKEPGSVEKTLSLLLENYI"
     gene            complement(13963..15729)
                     /locus_tag="PK125_07850"
     CDS             complement(13963..15729)
                     /locus_tag="PK125_07850"
                     /inference="COORDINATES: protein motif:HMM:TIGR01525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HPB38058.1"
                     /translation="MILVIGRKVIVDGIKSMFTLRFSDINLLMTVAIGGAIYLYQFEE
                     AVIIVVLFTLSEAMEDYGVTQSKKALEDLVNRTPKTALIKGQSEKTVIDDIDINTIII
                     IKPGDIIPLDGVIVAGNSFLDEAMITGEPLPKQKFTGDSVYAGSHNSQGYLEIKVTKS
                     SKDTTLAKIIELTYEAAEKKSHSQKFIESFAKFYTPAVVLTATLIVIIPVFAFDKEFN
                     FWLVQAITVLIISCPCALVISTPVTVFSAIGNATRRGVLIKGGKFLEELAKAKAIAFD
                     KTRTLTKGEPQVSDVFAYNEFSTDEVLACAAGLELFSEHPIAKSIIAKAEDANLEPHK
                     FCNFRAVAGKGVEGECLICDNPSHYLGNLQFVSDRSDGSVEQYVKDRVSKLESEGKTS
                     IVISEQKMVKGIIGITDKIRTESAKMIENISTLGLTPVMLTGDNKKPAVFVANQLGIK
                     DVRAELLPEQKDEEIRKMTEQFGHVIMVGDGVNDAPALARSSVGIAIGAVGSDLAVEN
                     ADIALMNDNIALIPYLISLGRKCSAKIRLNILSSVGVKAIFLILAILGYSSIAWAIFA
                     DVGMTMIVVLNGLSLFRFEYET"
     gene            complement(15903..16055)
                     /locus_tag="PK125_07855"
     CDS             complement(15903..16055)
                     /locus_tag="PK125_07855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB38059.1"
                     /translation="MKSKSDLPFIERYTIENILIIKPSIQMIIKITDGFGITLAEYIK
                     RLILKS"
BASE COUNT         4390 a         3693 c         3358 g         4774 t
ORIGIN      
        1 gtaaaggcat tcaggccgaa atttctacac acaaagaggg catattgcta aagggagata
       61 aagaggaact gacctatctc ttctattatc ttgttcaaaa cagtatggaa gctgcagaac
      121 cagataatat gcgggtagtc attaaatcag ggcttgaccc agactctcct tcctatgtta
      181 ggattgaaat ctttaataca ggagaaccac ccaaagagaa gacagaacaa ctgttttctc
      241 cttttttttc aacaaaagtg aaggggaccg gttttgggct tcccatcgcg caggttgtag
      301 tgagaaaaca ccttggacgg cttgatataa aacccatgtc cggtcaaggt acaaacgtta
      361 tagtgagctt gccgagagcc acattgtaat acaaaagtaa aaacttgtgg acaaaaactg
      421 gaaatatgaa gtcttgacta tcactgggtg aggttcacga tcctcatatt gataaaggct
      481 tcttcatcaa ggtactcatc atatgtcatt tctttcgcta ccatgctgct cccacaattt
      541 ggacaaaaat tctctggtac ctctccatca aggaaggaat agccacaaag gttacatata
      601 tactcatcgg gaattttcac gttgattcct ccttgtcatt ctgaagtaac acacttcaaa
      661 agtgaactta ttaaagatgg tacaatgaca tctgtgcaca caaaagattt gtcgatgtac
      721 aatttattta tttgcgatgc ctgccatgct gtcaggctgg caggcaagat atctttcata
      781 taacatgtgt tggtgattct aatatagcat gaatcagcaa gaaatgcacg caccaaagat
      841 agggaattga gcaaagtggt gagcggtgca ttattaaaag aactcgccaa ggataagctc
      901 gaagtttctt ttaataggtt tatctcccct aaccatttca ccatggcccg ggataagata
      961 ttctattcgg aacttagcta accgccttat gctttgtccc aaagctgcca tatcccctcc
     1021 cggcaagtct gttctcccaa ctcccatata aaaagcagta tctccggaaa tgagaaccct
     1081 ctctttttcc cagtaaagac agattgaccc tggagaatga ccaggggtcg ggataacctg
     1141 aagagttttg tcacccaggg ttagttgacc ttcctttaga aacagtttaa aaggtttttt
     1201 tggcgcctgg catcctgaca tgagaaaaag ttctttgccc tcttcctgga gataaccatg
     1261 ctcttctctg cttattgccc ggagaacgcc gttgtcaaaa tggtccagag cttcaatgtg
     1321 gtctggatgg ctgtgggtgc atacaatcaa tttgatggtg tcagcagatt ttccatctct
     1381 ggccataccc tccactacat ggttgaaaag atgcgaatga cctggatcaa ttaatgtagg
     1441 aaccggacca tcgataaaga ttgtattaca gttgttttcc tgataggagg tccagggata
     1501 gacataaaaa tcatcaaaaa gcttcattgt gtctcctata tcttgtatct ttcaaaatct
     1561 ttgtttgctc gctcaacttc gtgacggcaa attaaaccat tattcgtctc tactgcaatc
     1621 atcgatttct ggtttcgtcc ataatgagag ataatattgg caacgattgc tatgattccc
     1681 ttttcgttct ctccttttat gatgccaagt ggcccgtgga agtctatggg ataaacaagc
     1741 gtatacgggg gaatccacat ggaatgtaat cgttcatttt cccctttgtc cctgcccaaa
     1801 accaacttcg ttgttctgtt tattctgaaa tgtctgcctg tgttcagcaa atttatatca
     1861 agcatggtaa aattcttgtc atgggtaaga agatcctgta atctcatcga gtacaatgca
     1921 tcacagagca ggcaaccacc accagggtca ccaaaagctg aaagtccata tttttgaacg
     1981 aggccaaact ggaccgctct tcctctgccg gcaatgtcca aaagcaattc ccggttgacg
     2041 ataccctcta cttctggcat tgatggggca aagtgtttgg cagataaagg cctcaggatg
     2101 agcgatgtaa ggccactttt tttttcaata aggtctatgg tagaccttcg ctgagacatt
     2161 ggcctttgac ccagaacctc gccggtaatt acgaaaccag cgccctcttc tgccattacc
     2221 tccttagcct tacgaagcat gaaaatcctg caatcaatac acggattcat atgttttccg
     2281 tatccataca atgggttcct aataacatca ataaattcat aacctttttc ttttagaata
     2341 aggcggattc ccagctcttt ggctgttctg atggcctgaa ggcctttgga tctctctttt
     2401 ttgcttgaga agggagacgt gaagctaagt cccaccacct caatgccctg gtcgaggact
     2461 aatttggttg caatagcact gtcaagtccg cctgacacca gagaaatggc cttgctgctc
     2521 ataggtgaaa acgtaaaatc agcaaaggaa gcatactcac ccatctacct atcaagtata
     2581 ttccagtatc cgttcctcaa gcgtattcat ctttatccct gcatcaagcc ctggcaggga
     2641 ttctactggg ttagtgagga gaaatcttgc agagtggatc cattccttaa gtgtcgtagc
     2701 aatcgagaca gcctttgaat agctcgatat ggggaaagtt ggaatttctt tgccgttcaa
     2761 tctaatagct cctgatttta gttgtgcata gtttacctcg cccaggcttc tcggctctct
     2821 gttaggataa tcgtttccgt aatctacaac ttgtgcccag aagtcttcat cacttctcga
     2881 cgtaaaataa gccatttctt catcgatgat tggaatcggt atgccgatcc caacaaatat
     2941 ggttgcacca tatcctatga agcttgctcc ttttatatac tctgcgctca tttgttttgc
     3001 gtcaccaata acagcaaggg tgcctgcacc agcccggggt attccttcag ggcttctctg
     3061 tacatcaggg ttatgctggg taccatacca ggcaacatat ccaacaccac cgcccaggaa
     3121 caaacgcgtc cctataccca ccgtcctgta tttggggtca ttcaagaggg gtgacagctg
     3181 gcctgcacta caataattgg cactgttcag atgtggtttc aggacaccca tataggtgtg
     3241 aatcaccctg tcggtcgcat ttactgcaac attgtagttt tggtaatcat ttcttgggtt
     3301 atacaggtac gcctcattca cattcgtttt attaataaat gtttctatct tctttctcgg
     3361 gtaacagtct gtcccgtatg ccgtagcaac aagattgata ttttttccgc ttatgaaatc
     3421 ttcaatcaca tggccacccc cgtatttgaa agctcccgga taaacagtat ttctgggatc
     3481 atcatcaggg gatgctgtag ctccaatata aagatcaact gcagcaatac cacagtaagc
     3541 aggaacgtca ttcaagtaac atttccctcc gccaatcttg atccgtggct ttgaatggcc
     3601 aacgttgacg aacataccgc tactacacat ggggccaaag gttcctgttg taacaacatc
     3661 cacatattca gatgcttttt ttgttccttt gtccttggtt atacctatca tctcttcggc
     3721 ggtgacaacc acaaccttgc ctttcttgat cttttcatta atttcatcaa tggatttcaa
     3781 aaatgcctcc ttactcttaa tatccttttt atctgagccc tttcaatctt tcatgtaggg
     3841 tagaaacagt tcagtagaaa gatacctgtc tccaaggctg ggaacaatta caacgatcat
     3901 tttgtttttg tattcaggct ccctggctac ctcaagggct gcccacattg cagcaccaga
     3961 ggatattccg cagaaaatac cttcttcgtg aaccattctc cgggctgtcc gaaaagcagc
     4021 ctcattactt accttaacaa ttctgtcaat gacctttgta ttcagcacat ccggcacaaa
     4081 accagcaccg ataccctgaa tcttgtgggc cccgggctga cctcctgaga gtaccggcga
     4141 agcttcaggt tcaactgcta ttgcttgaaa ggttggtttt ctcgctttta gaacctcact
     4201 aactcctgtg atagtcccgc cagtgccaac accagctaca atggcatctg cagctccatc
     4261 agtgtcccgc catatctcct cagcagtagt ttttcgatgg atttccggat tggcagcgtt
     4321 tttaaattgc tggagcatga tagcctctgg gttatttttg ataatgtctt cggctttgtc
     4381 aatagcgcct ttcatgcctt gagcccctgg cgtaagaaca agctctgctc caaaagccct
     4441 gagcatggct tttcgctctc cgctcatagt ctcaggcatt gtcaggataa gcttatatcc
     4501 ttttatagca caaacaaagg caagtgctat tcctgtatta ccacttgtgg gctcaacaat
     4561 gattgagttc tcatttatga tgccactttt ttctgcagcc tcgatcatac tcacgccaat
     4621 cctatccttc acgctgccac atggattaaa gtactcaagt ttagccacga ttttggcatc
     4681 aagacccctc gcgagcttat tgattttaac cagaggggtg tttccgacaa gttgtgtaac
     4741 attttctgct attttcatcg ccatctccag caagtattat acaatagttc agccccaaat
     4801 ataagggttt tcaaaaaaca tggggttatt tgacgagaat aggttggcct gtaaaaccag
     4861 aataatctgt acacccgaga ttgaagatct tgttaatatc tgcgattcct tcttttacca
     4921 cgccatcagg cacaaactgg tttagaagac tttcacgcca aatttaattt cagcttctga
     4981 cctcctacca taaatgggct tgagatcgtg tggtctgcct tgcaaggccc ccttaacacc
     5041 aaccctaata agagcttctg cggagattct tgaaaatccc tcttttataa gataaacacc
     5101 atcagcagat gagagagaat caacacacat ctttaccccc gatccgaaac agaggcatgg
     5161 tgtttttagc atacgggcta cttctgcagg tttcagtgcc tgataatctg aaatctgctc
     5221 gatattttca tgagagacac ggtatagcgc ggcaaagacc tcacccttct ttgcatcgag
     5281 aaggggacag agaaaataac cgcggagaaa tgagaaagaa gaaacgagta catcaagggt
     5341 tggcacacca ataaggggaa tactctttcc agatgcaagt cctttacaga aagcaagtcc
     5401 cacgcgaatc cctgtaaatg atcctggacc aagggtggta ataatgagcg acaggtcatc
     5461 aacaccataa ccatggctta taaggatatt gagaacctca cgagggatga tctgagatgg
     5521 aggctgatgc ggctttatgt gtctctcttc caggagcaca tgctcacggg caagggcaat
     5581 atttaagaag tccagcgagt tgtcaagagc aaggacaagc ctatttagca ttgaaaaatt
     5641 tgctcagtcc gaaaaatctg tcaaagtcat tgtagaaagc aaagaccata attgcaatta
     5701 agagcgccag gcctaccttc tgcgcatttt cgatccattt ttgtgaaatt tttcgacgaa
     5761 tcacaacctc aataagacta aaaataatat ggcctccgtc aaggatcggg atcggcagga
     5821 ggtttattat tgcaagactt aagcttatgt aggcaacaaa gaatgcaaag gagcctgtac
     5881 cctgttttgc agttatccct gccgcctgga atattgataa cggtccacca acattccttc
     5941 ttgacatttt tccggtaata atcttccaca gtgcaattac tgtatcttcc ccaacgccgt
     6001 atgtataagc aggggttagg aggattgcct caaagatgtt atttgcctga acaccggagg
     6061 ccttttctgc ctcaatgcct attacttttc tccgcacaac agcgcctgca gcgtttgttg
     6121 tctcaatctc tttgggagta acagaaaatt cgagtagctg tccctgtctt ttcacctcta
     6181 tacgcaaagg cttgtcaaga gtttttgcca taaactgctg aacctctgca aaatacgcga
     6241 ccttttgtcc attgacggca gtgatggtat caccagggag gataccgacc tcagcagccg
     6301 gataatcttt gatcactccg gaaacttttg cgggtggtcc tgatggtgac gcaggaagca
     6361 ttgcaccgct tgataacatg acaaagaaaa tgagtcctgc gcacagaatg ttaaagaagg
     6421 gcccaaagaa aacgatgagg attttgacga ttgttggttt gttcgaatag gacctgtgag
     6481 cttcttcagc gcgtatttca tcttcagtcc cttcccccag caattttaca tagcccccta
     6541 gaggaaatac tgaaagggca tattctgtct cgccccaggt gcgcttccag agtttctttc
     6601 cgaaaccgag tgaaaaggtg atgactttta catctgtcag cttggccatg agaaagtggc
     6661 caagttcatg tacgaaaatc agaaaaccaa gaacaattat accgggtatt atattaaagg
     6721 caatactatt catattcttt ttgtattttc tcctctgtaa cctgttctgc ccactgatgg
     6781 atttccagaa ttgcctgtat gtcttccact accgctgaac catggtgttg ctcaagtgtt
     6841 ttttcgatca aaacagggat ttccgtaaac cttatttttc cttcaataaa tgcacgggcc
     6901 gccacctcgt ttgctgcatt cataacgatt accgagctat ctccatcagc aagtgcttca
     6961 agcgctattt tcagtgacgg aaaccggtct gtatctggtg gatagaaagt cagtttgaac
     7021 atttcgttgg tattgatggc cgcaaaaggc aggggccgtc tcttttcttc atttaacgca
     7081 tatgaaatag ggatcctcat atcaggatat gcaaggtacg caatgaacga gttatctata
     7141 tattctacca tgccatggat aatactttca ggatgtatta gaacatcgat atgggatgga
     7201 tcaatatcaa agagccaacg agcctcaatt acctcgaggc ctttgttcat cagggtggct
     7261 gagtcaaggg tgattttgtt acccattttc cacgtgggat ggttgagggc atctcgaggc
     7321 tgcacggttt ttagtttttc cttcttgtag tttctgaagg gtccacctga tgcagtaatg
     7381 atgagtcttt ttaaatcatc ctgtcccgag gttttcatga tttgatacaa ggcagagtgt
     7441 tcactgtcta caggtatgat ggcaccattt ccctggccca gcatcttcct tacaagtctt
     7501 cctgccataa ccatgctttc tttgttggca agagcaagta gtttgtgatt cttcagggcc
     7561 tcaatggtgg gttcaagacc aatacttcct ggcatggcgt ttatgacaat atgagcatcc
     7621 attccaacta aatctttaat gccacccatc ccttgaagca aacgtttttt tggaaatttg
     7681 acgtgcgata tgagctgatt gtcataaaca ctgacatagg caggtttgaa tctctgtatc
     7741 tgcgtgttca gcaggcttat gttgtcttta caggcaagcc ctactacttc aaaggagtgg
     7801 ctttgttcgt gtataacatc gagggtggcc tgtcctatag aaccagtgga accgagaaca
     7861 aggattttct ttttcatacc ctgatacctg taaggaactt gataccggta aagtagtgat
     7921 agagaaaagg cgctgtgaaa attaaactgt ctaagcggtc aagcatgcct ccatggcccg
     7981 gtaaaagaga ggaagaatct ttcacatcgc ataccctctt tcctgcagat tcgaatatat
     8041 ctcctatttg tcccagaaca ccgatgaaag cgccgattat tgttgactca acaatcccgg
     8101 tccccatgaa ttcactcgat aacgccataa tgatcattgg gcctattatc gctcccaata
     8161 accccacata agtcttcttc gggctgatac gtggaacaag aagttttttt ccaaatgtct
     8221 tacctaaaaa atatgcagca atatcacttg cccagagggc aaggattagc aaaaggggaa
     8281 aaatagaatt cacatccttc aggaggtaaa gatagaagag aggaagcaca ataaaaatct
     8341 cactgaggag taaagtagag actccccgca ttatatttag gttaacatta tcttttttga
     8401 tgttctcccc ttgtaggaat tgtgtgatga gaaaaatgac aggggagaag agcagccaca
     8461 tgagaaaaat atggatcgac tgggtataaa gcggcacaaa gccgagaata gcaagaaaaa
     8521 gaacaagata acgttctcca atacctgaca tggttatcag ttcataaatt gagagacagg
     8581 ctacaagcgc caggagaaca aaaaaccatc ttgcagggag caaataaaag agcgcaacaa
     8641 tgatcggtgc aagacagagg gtagtcaata ccctttgttt agtatcattc aatcaatatt
     8701 cctcaacccg gccaaatcgc cgttctcgtt ttgtgtattc tttgagagct gaaaggtagt
     8761 gtttttttcg gaagtttggc catagcactt ttgtaacata gatctccgtg taagccgtct
     8821 gccagagtaa aaaattgctg agcctcattt ctccgctcgt acgtataagg aaatcaggat
     8881 cgggtatcgt accggtataa aggtattttt tgaagacatt ctcattgatt cttttcaccg
     8941 atcctttttt gacatcttcg aagagcgaca caatagcatg aaggatttcc tgcctgccac
     9001 tgtaactcag ggcaaggttg agggtcaaat ccttattttt tgctgttttt ttcataaccg
     9061 aatacatttt ttcctggagc tttttgggaa aatctttcat ttctccaatc attttgaatc
     9121 gtatgccctt ctcaagcaaa aggggaagtt ctttgtcgag gtaaaccccg agaagtccca
     9181 tgagcttttg tacctcttcc ctgggtcgca cccagttttc tttggaaaag gcatataacg
     9241 tgaggtacgg tattcctaat tccctggttg cggttactat gtcgcgaaca gattcaatgc
     9301 ctgttacatg tccttcaatt cgtgggagat ttttggtctt tgcccacctc ccgttaccat
     9361 ccataataat ggctacgtga cggggaagtt tctcattgtc aaggcaatgc ataatgtaag
     9421 attagatttc gaggatctcc ttttccttct ctgcatatgt cttatctgca atttctataa
     9481 atttatcagt aagtttctgg acatcttcct gctgtttaaa ggcatcatct tttgacagaa
     9541 gtttatcttt ttccatcttc ttgagcttgt catttatttc tcttcttgca tttcgtatgg
     9601 ataccttagc gttttcaagc atttttccgc caagttttgt aagatccttt ctccgctctt
     9661 cagtcagctt tggaaatgaa aggcggataa tcttgccatc cgaagccggg ttgaccccca
     9721 gatcagattt ctgaattgct ttttctatct ctccaatggc agagttgtcc cacggttgga
     9781 tagttatagt tcgactgtca gggacggcta tagtggccac ctggttcaat ggggtgggct
     9841 gattgtaata aacaattttg atgtcctcaa gaagtgaaat cgatgccaca ccagtcctca
     9901 gttttgaaag atcttttttt aatgctgtca aagatttgtt tagcttatct tccagttcat
     9961 caatgatttc atttttcatt cttatcacct caccatagtt ccaacacgtt ttccctggat
    10021 tgcttttttc atgttatctt ttccccgcaa attaaatacg attattggca gactgttttc
    10081 catgcaaagg gtaactgctg tcagatccat taccccaagt tttttgttaa ttacgtccat
    10141 gtacgtaatc ttctgaaata aagaggcatt tttgtgggtt accgggtcac agtcataaac
    10201 accatcgacc tttgtagctt ttaaaagagc ctcggcccca atttcaactg ccctcagtgc
    10261 agcagcagta tcagtggtaa aatacggatt acctgtgccg catgcgaaaa ttaccacctt
    10321 tccttcgaca aggtttttga tagcttccct ccggtcgtac ggtgcagcaa ccttttctat
    10381 attgatcgcc gactggatgt tcaccggtac gccgattttc tcaagcgttt cctggagggt
    10441 taatgcattc agtagggtag caatcatgcc catataatca gcagttgccc tctccatgcc
    10501 ctgtttttca gctttcattc ctctgaagat attgccgcca ccaacaacga tacctatttg
    10561 cgtacccaaa tctttgacat tcttgatctg aaggcataca tcttttacag tggcagggtc
    10621 aatgccgtaa ccttccccgc ccatgagtgc ttcaccgctc agttttaaga ggattttctt
    10681 gaatgccaca ctactcttcg atggtttctc caagctgaaa cctcacaaaa cgattcacaa
    10741 taatcttctc gcccatcttt acgatgagtt cttcaagaag ttcctttact gttatgtcct
    10801 ggttttttat gaagggctgc tcaatgagac atacttcctc gtagaatttc tccattttcc
    10861 cgtcagctat cttgtcaata attttttctg gttttcctga ctccatagcc tgctttcggt
    10921 aaatattttt ttccgcctct tttgcatctt cagggacatc ctccctttta acataaagag
    10981 ggttcgatgc cgtgatttgc atggcaatgt ctttcgcaaa ttcctggaac tcttttgtgt
    11041 tggccacaaa gtctgtctcg caattgatct ccaccatggt gcctactttt gcaccggtat
    11101 ggatatagga ggcaatcaca ccttctgatg ctgtcctccc catccttttc tggagtttcg
    11161 caagtccctt ttcacgaaga tactcgattg ccttttccat gtctccggca gcgttcttca
    11221 gggcttcttt acaatccatc aggccaacac ccgttttctc tctcagtttt ttgacaagtt
    11281 cagcacttat ttccatgctc catctcctcc ttattcactg ccctcatagt attcttcgta
    11341 gcgttcttct agaacacttt cgtcaacaaa aggcttaggt tcttctccgg gttcttcctt
    11401 accttgaaat tcctctacgt aaagcgcttt tccttcaagg atagcgtcag ccaccttcat
    11461 ggtaatcagt tttattgctc ttatagcatc atcattgcca gggataacaa aatcgatatc
    11521 gtcagggtca cagtttgtat ccactatgcc gatagtagga atacccagtt tcttcgcttc
    11581 gctaacagct atgtattctt tcttgggatc tactacataa acagcgcccg gaaggcgatc
    11641 catccccttt atgcctccaa tgtttcgttc aagtttcgct atttccttat cgataccgag
    11701 ggcctctttc ttgggcaaag ctttgaagat gtcactttcc ttcaattcct catattttct
    11761 cagtctgtca atactttttt caatggtttg gaagtttgtc atggttcctc caagccatcg
    11821 tgaattcacg taaaatgagc cggctcgctt tgcttcttcc tggattgcct cctgggcttg
    11881 cttcttggtc ccaacaaaaa gaatatactc gttgcgggaa gctacatctt taacaaaatt
    11941 atacgcctcc ttgaacatct tgagtgtttt ctggagatca atgatgtaga tgccatttcg
    12001 tgcaccaaaa atataaggct tcatttttgg gttccatctt tttgtctggt gcccgaaatg
    12061 aacaccggcc tctaaaagtt gtttgatggt aagattagac attgattcct cctggttatt
    12121 cgtccgcttt tcgcatttta acaacagaca gagaattctg caccacgttg tcaattgaaa
    12181 aggcgtgtgt atttaactgc ctttattaat tatcatatga tttcaaatat ttcaacaaaa
    12241 aacgagtatc tcaatgcgta acacatcctc ctgattccta aacaacaacc tctgttgctg
    12301 ttgaacagca taggttttta tggtatggta gtcgaaagga gatatacatg gcttcaaaca
    12361 caaaaaagac tgaatctatc aggaagagaa agagagcggc tcagggcaag aaacgaaaga
    12421 aagtgatgtc caagatgtca acgccggcct tcccgatcca cccggacaaa accgaataac
    12481 attcaaagca ctcatgcggt acgaaatagg acccatcagg ccaccaaatg aggcatatag
    12541 tctccttgtg aggtttacgc ggaactgtcc ctggggcaag tgcgctttct gcagatctta
    12601 caagcagcgg agatttgaaa agcgcagcgt agtagaaatc aaagaggata ttgatatcat
    12661 tcgtaaaatc aaggatgaaa ttgttgcgct ctcctggcaa aaaggttttg gtggagagat
    12721 caacgacagg ctgatcgacc ttattttttc aacaccggcc tataacgaat attttaaggg
    12781 tattgcaatc tggctttatt tcggggggaa gaatgttttt atccaggacg caaactctct
    12841 ggctatgaaa cctgctgatt ttatcgaagc cctgacatac ttgaaacaac agtttccctc
    12901 catcgacagg gtcacttctt atgcccgtgc ccgcacaatt gctaaaaggc tgactgtgga
    12961 tgaccttaca aggatgagag aggctggatt aacaaggctc catataggcc tcgagtcggg
    13021 aagcaactcc ctcctgaaat acataaacaa gggagtgaca aaagaagagc acatagagtg
    13081 tggcagaaaa gtgaaagaat caggcattga actttctgag tatgtggtcc tcggcctcgg
    13141 gggtaaaaag tggtggaagg aacatgctga agaaactgca gatgcattga acaggattga
    13201 tccagacttc atccgcttca gaacactgaa ggttttgcgg gatatgccgc tttatgagaa
    13261 ggtagaaagc ggagattttg aaatcctaca cgaagaagag atactgattg aggagaagca
    13321 tctcatagag aggctcgaag ggatcaacag caccataaaa agcgatcaca tcctgaatct
    13381 ccttgaggat gtggatggca aactcccgga agataaagaa cgtattctgt ctgttattga
    13441 cgattatttt tcgctgaatg aagaacagcg gcttgtttat cggttcggga gaagggcagg
    13501 gatgtaccga agtataaatg atctgaatga tgaactcacc tatttacgca tcaagaagac
    13561 aatcaaggag atagaagaaa aagagcccgg cagcgttgaa aagacactgt cactgctcct
    13621 tgagaactat atctaaacct aacaaaaaag cattcggaac aaaataattc cagaattcac
    13681 aattgggata agcatcatgt atcgctcgta cgaaaaaagg gtattcctat ttcccggaac
    13741 atcaacgaca cgaacccccg gcaagaagct gttatagtac tgaagattgc ttgagatgag
    13801 ccaaaaaata gagaccttca atgttatgga atgagccttt cgggaagtcc gcgggagaac
    13861 cagaccccat gagctttttt aacaatacca gatttgattg ttttcaacag cagtgagttt
    13921 gaaatgtcat aatgatgcat gctatcaaag aatggattag agtcatgtct cgtactcaaa
    13981 tctaaaaagg ctaagcccgt ttaaaacaac aatcattgtc attccgacat cggcaaagat
    14041 ggcccaggca atactgctat atcccagaat cgccagtatc aaaaagatag ctttcacacc
    14101 aactgaggac aggatgttaa gccgaatttt tgccgagcat ttacggccta aagagataag
    14161 ataagggata agggcaatgt tgtcgttcat cagggcaatg tctgcatttt ctactgccag
    14221 atcagagccg accgctccga tagcaatacc gactgacgac ctggctaatg ctggggcatc
    14281 attaaccccg tctccaacca taattacgtg accaaattgt tcggtcattt ttctgatttc
    14341 ctcatctttc tgctcaggca acagctctgc ccgaacatct tttattccta attgattggc
    14401 cacgaacacg gctggctttt tattgtctcc tgtgagcatc accggagtca ggccaagagt
    14461 ggaaatgttt tcaatcattt tagcgctttc tgtgcggatt ttgtcagtta tgccaattat
    14521 acctttgacc attttttgtt cgctaataac aattgaggtt ttaccttccg attccagctt
    14581 gctgactctg tctttaacat attgctcaac cgatccgtca ctccggtcgc tgacaaattg
    14641 aagattgccg aggtagtgcg aaggattatc gcagattaaa cactctcctt ccacgccctt
    14701 gccagctaca gcccgaaaat tacagaattt gtgcggttct aaattggcat cttctgcttt
    14761 ggcgataatg ctcttggcaa ttggatgctc agaaaaaagt tcaaggccgg cggcgcaggc
    14821 cagtacttcg tcggtagaga attcattgta agcaaagaca tcggagactt gcggctcgcc
    14881 tttggtcaga gtgcgggttt tgtcaaaagc gatggccttg gctttggcca gttcttccaa
    14941 gaatttccct cccttaatca gaacacctct gcgtgttgca ttgccgatag cagaaaaaac
    15001 tgttaccgga gtagaaatca ccagggcgca cggacaggaa atgataagca cggttatagc
    15061 ctgtacgagc caaaaattaa actccttgtc aaaggcgaat accggaatga ttactatcag
    15121 cgtggctgtt agcacaactg ccggagtata gaacttagca aaggattcaa tgaatttctg
    15181 agagtgggat tttttttctg ctgcttcata ggtcagttcg ataatcttgg ccagagttgt
    15241 gtctttggaa cttttggtaa cttttatttc caaatatccc tgactgttgt gcgaccctgc
    15301 atagacactg tcaccggtaa atttctgttt tggcaaaggc tcaccggtga tcattgcctc
    15361 atccagaaag gagttgccgg caacgattac accgtcaagc gggatgatgt ctcctggctt
    15421 gattatgata atggtgttta tatcaatgtc atcgataaca gttttttcac tttggccttt
    15481 gatcaacgcg gttttagggg tcctgttgac caggtcttcc agcgctttct tgctttgtgt
    15541 tacgccgtaa tcttccatgg cctcactcag ggtgaagagg acaacaatga ttactgcctc
    15601 ctcaaactgg tataaataaa tcgccccgcc aatagccacg gtcatcagca ggttaatatc
    15661 ggagaatcgg agtgtaaaca tgctttttat gccatcaacg ataactttac ggccaatgac
    15721 cagaatcatc aacagagaaa gcggggcttt cacccaaatg ggaaaatgga tggaaaagaa
    15781 agaaagaatc tcaaaaggga ggattaagag gaaggctccg atcagccatc gtattttttt
    15841 attatcaagc aggttctctt ttgggggctc tgtgctgctg gttttattgt tgttacaaca
    15901 gttcatgatt ttagaatgag tcgtttgatg tattcagcaa gtgtaattcc aaacccatca
    15961 gttatcttaa tgatcatctg gattgagggc ttgattatca ggatgttttc aattgtgtat
    16021 cgttctataa acggaagatc agatttgctc ttcaatttat cctctgtcaa tgtctcttta
    16081 tattattacc tgcttcgtcc catacccaac tattttatca taatttccct ttcctcatca
    16141 cacagccagg cacatcaggt atggttggca gcattgtctc ccagccaggg gttgacgggg
    16201 aaggacaaag agccg
//
LOCUS       DAOOMC010000064        16020 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdus sp. isolate AMR_MDS_2685
            NODE_3853_length_16020_cov_6.953085, whole genome shotgun sequence.
ACCESSION   DAOOMC010000064 DAOOMC010000000
VERSION     DAOOMC010000064.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864760
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdus sp. (wastewater metagenome)
  ORGANISM  Syntrophorhabdus sp.
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae; Syntrophorhabdus.
REFERENCE   1  (bases 1 to 16020)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16020)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,729
            Genes (coding)                    :: 2,708
            CDSs (with protein)               :: 2,708
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 7 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16020
                     /organism="Syntrophorhabdus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3853_length_16020_cov_6.953085"
                     /isolate="AMR_MDS_2685"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2699756"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            790..1008
                     /locus_tag="PK125_07860"
     CDS             790..1008
                     /locus_tag="PK125_07860"
                     /inference="COORDINATES: protein motif:HMM:NF014304.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PC4/YdbC family ssDNA-binding protein"
                     /protein_id="HPB38060.1"
                     /translation="MLIGKIDNKKHEKLLVTVRELKGTKMIDFRMHHLNVDGRLIATQ
                     AGVTLTVDQVGRAIELLEEAKKRLTDQN"
     gene            1024..1149
                     /locus_tag="PK125_07865"
     CDS             1024..1149
                     /locus_tag="PK125_07865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB38061.1"
                     /translation="MDYNANNLEWFPSEGKTDLTVFKDNTFKGLYGVELWAFSVD"
     gene            complement(1445..2047)
                     /locus_tag="PK125_07870"
     CDS             complement(1445..2047)
                     /locus_tag="PK125_07870"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HPB38062.1"
                     /translation="MVAKIAFVKTFNKGYMVFEEGEKRDTLYIVLKGRVKISLYDEDG
                     REYILDIISKDGFFGELSLFEELSGFANVMTLEHCELLVIRRKDFMGILRDNNDFALS
                     MIKELSKRLRAANEKLKRFAFLGVEGRILEYLMDIGQKSGIKVKDRIIIESGPTQVEI
                     ASACGCSRETVSRMIKSLVEKGRLSVLKKQYTIRPVYEPM"
     gene            complement(2136..4763)
                     /gene="alaS"
                     /locus_tag="PK125_07875"
     CDS             complement(2136..4763)
                     /gene="alaS"
                     /locus_tag="PK125_07875"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HPB38063.1"
                     /translation="MKSNEIRKKFLEFFNENGHTIVSSSSLIPDKDPSLLFVNAGMVQ
                     FKNLLLGIEKREYVRATTCQKCVRAGGKHNDLENVGKTLRHHTFFEMLGNFSFGDYFK
                     EDAIAYAWKLLGDIYQLDKKKMWITVYKDDDEAAKIWKSIGIREDRIVRLGEKDNFWS
                     MGEEGPCGPCSEILYDLGEHVGCKRPSCAVGCDCDRFLEIWNLVFMEFDRSGDGKMTK
                     LPRPSIDTGMGLERIASIMQGKLGNYETDLFRPIITRLEDVSHNVYGENEKTDIAMRV
                     ISDHIRGATFVINDGVLPSKDGRGYVLRRILRRALRYGKKIGIEREFLSDLSSTVVDI
                     MGDVYPDIKNNHPYIVRVIKGEEERFIETLSTGMKVYDEFVDEISSKGESVMSGEMIY
                     KLYDTFGFPLDITTEMAQEDGLSMDMDGFARSLEEQKERSRTSSRIKGEGLDQVHVSV
                     LKQGISSEFLGYDMVRIPEARIITLIKNDTMVDEIRAGETGEAFFDQTPFYAESGGQV
                     EDEGTVDWTGGTARVTAAKKIKEDLFSHTIVVEKGILKKNQVVDLSINTERRKSIARN
                     HTATHLLQYALRQILGDHVKQSGSLVDKDRLRFDFTHFQAMDESEVARVEDIVNEKIM
                     EGIDVCTQKKSREEAMREGATALFEEKYGDEVRVVSISDFSAELCGGTHVRNTGEIGS
                     FYIVSEGSLASGVRRIEAVTGKSALDYKRKMDATIRGIARVANTEPERVRERIENLVA
                     ELNAQSREMEKLKDELTGYKVEDAIKGASEKDGVKVVSLFVPNARAEDLRKVTDIIRD
                     RVKSCVAVVGTRDNVKGMVIVAVSKDLLGKYNAGKMMKKMMEHYGGKGGGGPQIAQGG
                     VPGDKIKAALSSVIELMDN"
     gene            complement(4760..5776)
                     /gene="recA"
                     /locus_tag="PK125_07880"
     CDS             complement(4760..5776)
                     /gene="recA"
                     /locus_tag="PK125_07880"
                     /inference="COORDINATES: protein motif:HMM:TIGR02012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HPB38064.1"
                     /translation="MAAKEETGNKAKAIDMAVTQIERLFGKGAIMKLGEKALEEVPVV
                     STGSMALDMALGIGGLPRGRVVEIYGPEASGKTTLALEVVSEIQKGGGTAAFVDAEHA
                     LDISYAKRIGVNTDDLLISQPDTGEQALEITEILVRSGAVDVIVVDSVAALVPRAEIE
                     GEMGDAHVGLQARLMSQALRKLTATISKSLTTVIFINQIRQKIGIMFGNPETTTGGNA
                     LKFYSSVRLDIRRIGAIKDGQDVVGSRTRVKVVKNKLAPPFKEVEFDIIFGEGISKEG
                     EMVDLGVEAGVIEKAGAWYAYGETRIGQGKENTKEYLKKHQEVAKEIENKILEHYEMK
                     KAEQ"
     gene            complement(5927..6508)
                     /gene="thpR"
                     /locus_tag="PK125_07885"
     CDS             complement(5927..6508)
                     /gene="thpR"
                     /locus_tag="PK125_07885"
                     /inference="COORDINATES: protein motif:HMM:TIGR02258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008664 - 2'-5'-RNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HPB38065.1"
                     /translation="MRVFLALEISPSVKEYLQGIIKIMASRITGVRWVKSEGQHITLK
                     FFGELDEAMVENIRTRLLSIEDKFEPFETTIKGIDAFPNKRRARVIVVTLEKGVDIAK
                     AIFNDIEVALLPLGFEGEKRDYTPHITLGRRKEQSPILERDIPDLNGMGFIVDRLVMF
                     RSTLTPQGAIYSPLWEIGFKSRKSETGGNDEKT"
     gene            complement(6505..6981)
                     /locus_tag="PK125_07890"
     CDS             complement(6505..6981)
                     /locus_tag="PK125_07890"
                     /inference="COORDINATES: protein motif:HMM:TIGR00199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CinA family protein"
                     /protein_id="HPB38066.1"
                     /translation="MIEAKIGRILRERRMKIAVAESCTGGHIANRITNIQGASDYFDI
                     GFITYSNDSKVLYLFVPEGVLTKNGAVSAEVAKAMAEGLRETTKAHIALSVTGVAGPG
                     GGTPEKPVGTVFAGLASKDSTVVRKFLFSGDRISIKEQTSEEALKLVLDYLEGRLS"
     gene            complement(6978..7439)
                     /locus_tag="PK125_07895"
     CDS             complement(6978..7439)
                     /locus_tag="PK125_07895"
                     /inference="COORDINATES: protein motif:HMM:NF016489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HPB38067.1"
                     /translation="MTKRSTATKNNVFHTFVTLGPIGYLPYAPGTYGSALACLLLYFL
                     PSVFAHPLFVFIFIVVTLIVLNNLAFEGKDPRYIIIDEVAGMFVTMAGHNITLFSLLA
                     GFVLFRFFDIKKPYPIKRVETLRQGYGIVGDDVVAGVFANIIIIIGERILT"
     gene            complement(7408..7782)
                     /gene="hisI"
                     /locus_tag="PK125_07900"
     CDS             complement(7408..7782)
                     /gene="hisI"
                     /locus_tag="PK125_07900"
                     /EC_number="3.5.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017406167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-AMP cyclohydrolase"
                     /protein_id="HPB38068.1"
                     /translation="MNQIKWDEKGLVPVVVQDINTKDVLMVAYMNQDAYELTKKTGKA
                     HYYSRSRKKLWLKGETSGHFQEVKSTYIDCDNDTLLIMVDQKVAACHTGFWSCFYRVW
                     KDGWNIVGTKVFDDKEVYGDKK"
     gene            complement(7943..8650)
                     /locus_tag="PK125_07905"
     CDS             complement(7943..8650)
                     /locus_tag="PK125_07905"
                     /inference="COORDINATES: protein motif:HMM:NF013168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]
                     imidazole-4-carboxamide isomerase"
                     /protein_id="HPB38069.1"
                     /translation="MKTLFAMDLIDGECVRLVKGDFSKVTVYSKNPVDKIEELVQRGA
                     RDFHIIDLDGARTGNRVNTEIILKIREKVPGYMEVGGGIRREEDIRDYTSAGINGIIV
                     GTQALEDALFFANLATYNNIVLGLDVLDGKPMVRGWKSAINRDVQEILNEAKQVGIMA
                     ILCTNVAKDGMLAGPDYDGMKKMMQLTDIPLIASGGVTHIDNVKKLRDLGVWASILGK
                     AVYEGLIKVEEAIQYAD"
     gene            complement(8647..9207)
                     /locus_tag="PK125_07910"
     CDS             complement(8647..9207)
                     /locus_tag="PK125_07910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB38070.1"
                     /translation="MIPIKMFREKAPLIFATVFLLLSFAGTNNVHAIGDLGIVVEKMS
                     GSPGEIKKCSVCGRYMKVGNIPKGAEATINNILRESFSERIGGYGKDKNNGNYVHLLI
                     YRYEERIGGNLGVDKPAGAGFHMHLIENNVLKRVFTFDEDQEALSENLFNLGKFLRRG
                     AKWLTVEELCRDAISRGLDIILEDYR"
     gene            complement(9257..10483)
                     /locus_tag="PK125_07915"
     CDS             complement(9257..10483)
                     /locus_tag="PK125_07915"
                     /inference="COORDINATES: protein motif:HMM:NF013715.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HPB38071.1"
                     /translation="MKKFIILLSLLGPAIITSNINNDAGGIAIYSIAGARYGYNLLWT
                     LIPTVFLLIVYQEMSARMGMVTGKGLADLIRENSGIRVAFWVMLSLFVTNVGNTMAEF
                     SGWAASMELFGASKYISVPIGAFFVWFLVTRWNYSIFEKIVLSVCLIYSTYIISAFLA
                     KPDWGEVMQKTVTPSIEWSSGYLIMIISIIGTSITPWQQFYLQAGVVEKGLNEQDRWA
                     SKVDVIGGGIMMGVVAFFIIVACGATLFPAGIHIHTAEDAALSLKPLAGKYAYILFAI
                     GLANASLFSACILPLATTYYICEAMGWETGTGKRFKEAPQFNTIFTIIVILGASIILI
                     PNIPLIKVMWFSQIINCLLLPLILIFMLMLVNNEELMGEYRNSHWMNMVTYVSTGLLI
                     GLNLILLYNTCIDAMR"
     gene            11156..12352
                     /locus_tag="PK125_07920"
     CDS             11156..12352
                     /locus_tag="PK125_07920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB38072.1"
                     /translation="MLVFCILSFFVSFPYALAINVASVEKFLDNAAFDAWDPNSNDLL
                     FLRKDEKGVLQIFKVRENSINPESDKVCISCNAQRAVGFKLSTVPLIHKGASDWHPSG
                     EWFITQGEIPDNISWKQEKRIPGARLNAEPETGWWNNLFLVSKDGKIWIELTKFTNHD
                     INSGILSPTFSKDGKMIAWAERIGGSRPFDQYPNGRWLLKTAKIHVNGEDTRIYDIKV
                     HPPQDGATFKPQGWSSDGKLLLATDIGYGELPYPGYRADIYEVRFDPDGTIKNMKNLT
                     GSKDFLEHYASYSPDEKLIAFTTNNFDPQYEKRMNDAWKKDGPRFSHFIVRNLFTDLF
                     LMDRDGKIVKRITNFVDQEWKGTHPIVAKNAWSKDGKTIILGLVLRSNITGKKEGEMI
                     YRIRLE"
     gene            complement(12779..13063)
                     /locus_tag="PK125_07925"
     CDS             complement(12779..13063)
                     /locus_tag="PK125_07925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HPB38073.1"
                     /translation="MPGKTVQTETAEAFGRTFIKGNIPEINSVRCLGCRNCVNLCKKL
                     GPNVLDIVDGVAKPVRPQNCIGDGACMLSCPTKAIFLMSKYDPAAPPVAV"
     gene            complement(13005..13223)
                     /locus_tag="PK125_07930"
     CDS             complement(13005..13223)
                     /locus_tag="PK125_07930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB38074.1"
                     /translation="MGKQFMGGIASDVVKRTDHSHHVAQVVDAEPAVVGITGLADYTK
                     KYEIKGGLYHARKNSADRNRRGLWAYIY"
     gene            complement(13288..13734)
                     /locus_tag="PK125_07935"
     CDS             complement(13288..13734)
                     /locus_tag="PK125_07935"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HPB38075.1"
                     /translation="MVKPKKILVPTDFSEHSNRAFERALDVAKQNDSEVVLLHVIDQD
                     IQACTVDYCLTEDEVTKMEKRVMENAKNSLHQIQTKFPLSGEVKTSAEVVQGIPYEEI
                     LKFQEQNGVDLIVIASRGRSAIAKYFLGSVSSNVLKGAKCETLLVK"
     gene            complement(13787..14233)
                     /locus_tag="PK125_07940"
     CDS             complement(13787..14233)
                     /locus_tag="PK125_07940"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HPB38076.1"
                     /translation="MFKPKKILVPTDFSDHADRALEKAIDIAKEEGSEIILFHVIHED
                     FQTCVVDYCFTADEIDAVKKRVFQAASENMQKQLDKFPSSKEVKVSIEARNGIPYEEI
                     IKLQEEKGADLIVISPHGRSAIKAFFMGNVASRVLKHAKGEVLLVK"
     assembly_gap    14374..14473
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<14474..15316)
                     /locus_tag="PK125_07945"
     CDS             complement(<14474..15316)
                     /locus_tag="PK125_07945"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HPB38077.1"
                     /translation="MSKGYWSEISTGHCVVSVNANVSIDWALASLWNRAGIIQTKKQD
                     ERNGFSSDPKTSRAVEQVVLPAVGPFKSVMQHGAVLEEEIEKKALEQFYLGRIQDSHA
                     RGVNLIYTLMILNGKMPMKMEDFDLNDILRGIVPFISPIMRDDITIQVDIAEDSLPMK
                     GDMRFIKQAVGELILNGRDALPFGGTIALVTRKVKVRMNSPDSTQHKIVECALLSVVD
                     TGKGIDPQLKSHIFEPFFTTKPTGTGHGLGLTLVNQIVQAHKGCFKIISRKEEGTSAR
                     MFLPL"
     gene            complement(15542..>16020)
                     /locus_tag="PK125_07950"
     CDS             complement(15542..>16020)
                     /locus_tag="PK125_07950"
                     /inference="COORDINATES: protein motif:HMM:NF014950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HPB38078.1"
                     /translation="EDIKELTIHYLRKFCEGYGVAMKGVTPEFFDALTGYEWLGNVRE
                     FANAMDTAVCSALHEPTLYPKHLPTYLRVKLAHAALKETKKPVEPVLPGGGKIEEFTT
                     LSSFRGDIIDEAERQYLVELIARTKNNVKEICRISGLSRSRLYALLKKHRLIKVKP"
BASE COUNT         4315 a         3853 c         3106 g         4646 t
ORIGIN      
        1 gcaggaacga agcggctcaa ggtccttaag atcgtgtatc agccggagag ggatggagag
       61 agaggtggta aatttttaga ataatcaggc aatgtttcca caccctataa tattccagga
      121 tttaagctaa atccggagaa aactacgatg tttgtcatta cgcaaaggac taaacatttt
      181 tgaatgtcag gcaatctctg gacaagattt ataaatcgat aaggtgtata ggtacaatat
      241 tctcttatcg ctttcgttag caggtgccac ggagacacga tcactcgtct tttggcgaga
      301 accatgtcag gaggtaaaaa tgggagacag aggtggtaaa aaaaataaag ataagcggca
      361 aaaacagaaa gctcgcaaac tcgcagataa tttgaaagaa aagaaggaaa aggaatccaa
      421 aggcgttaca aaataagtct gacaggatta gcagacagca aatgccggga aattgagtcc
      481 gtccttggca ggagggcagt tgatcacggg tgtggcaaag ccaggggcca caacagggaa
      541 aagactggtg tttgaagaac aaaaccatct gattaacatg gcctgagcgg cagtcaacct
      601 taaaattggg ggcttctcgg cgcttttgtg tgtcctgtca agggtataca tgcactcgat
      661 gggcccaaag aaaccaactt cagggaacta aaaaggcatt gcagatcagg aaacgtgggg
      721 acagggctat gcaggtacgg atctttcttc tgcttgattg gctaatgtgc ccaaccaaat
      781 ggagatacta tgctaattgg aaaaatcgac aacaagaaac atgaaaaatt acttgtaacg
      841 gtgcgggagc taaaaggtac aaaaatgata gacttcagaa tgcatcatct gaacgtggat
      901 gggcgcttga ttgctaccca ggctggggta acattgacag tggatcaggt tgggcgggca
      961 attgagttgc ttgaggaggc aaagaaaagg ctgaccgatc aaaactgagg gggcttaatt
     1021 cccttggatt acaatgcgaa caaccttgaa tggtttccat ctgaagggaa aacagacttg
     1081 actgtcttta aggataacac cttcaagggt ttgtatggcg ttgagctttg ggcattctct
     1141 gttgattgag ttttgtatct ttgttcagga atccggcaaa acacccggaa aggcttaaac
     1201 aacagcatta caaaaaatat agatagtttt tgtggctatg acaaattttg tcataacata
     1261 tctggatcca tagcattacg tcgtttacat atggtcttcg gggagaatga gctggcaata
     1321 attatacttc catcttggca aacaagcctg gagaaataac gacttcattt ttgacgttag
     1381 ggattggtgt atggatgacg gcaatcaagt gttaattcgg aatagagatt tgtagcagtg
     1441 ttcttcacat aggttcgtaa accgggcgaa tggtgtactg ttttttaagt acactaagac
     1501 gacccttttc tacgaggctc ttaatcattc tcgaaactgt ttctcgtgag cagccgcagg
     1561 cgctcgctat ctcaacctga gtaggtccgc tctcaataat aatacggtct tttactttta
     1621 ttcccgactt ttgacctata tccatcagat attccaggat acgaccctcg actccaagga
     1681 aagcaaatct tttcagcttt tcgtttgctg cacgaagcct tttcgaaagc tctttaatca
     1741 tgctcagggc aaaatcattg ttgtctctga gaatccccat gaagtccttt cttcgaatca
     1801 ccagcagttc acaatgttcc aaggtcatga catttgcaaa gcccgacagt tcttcaaaaa
     1861 gggaaagctc accaaagaac ccgtccttac tgatgatatc tagaatatac tccctgccat
     1921 cctcatcgta tagagagatt ttaactcttc ctttgaggac aatataaagg gtgtctcgct
     1981 tttctccctc ttcaaaaacc atgtaacctt tattgaaggt cttcacaaat gcaattttag
     2041 caaccatctc aagctcctgg tcgcttaaag tggaaaacaa actgacacta cgaatatttt
     2101 ctgcgtacag tttcataact gttcacccaa atgtgttagt tatccatcag ttcaatcaca
     2161 gagcttaatg cagctttgat tttatccccc ggtacaccgc cctgtgcaat ctgtggacca
     2221 cctccgcctt tgccgccata gtgctccatc atcttcttca tcattttccc tgcattgtat
     2281 ttaccgagta gatccttgct cactgcaaca atgaccatcc ctttaacatt atctcttgtc
     2341 ccaaccacag caacacaact tttaaccctg tctcttatta tatcggtaac ttttctcaaa
     2401 tcttcagctc tggcattggg aacgaacaat gaaacaactt tcacaccatc cttttccgat
     2461 gcccctttaa tagcatcttc caccttgtaa ccggttagct catcttttaa cttctccatc
     2521 tcccgtgact gggcattgag ttctgccaca aggttttcaa tccgctccct aacacgttcc
     2581 ggttctgtgt tggcaacccg ggcaatcccc ctgatggtcg catccatctt tcttttgtaa
     2641 tctaaagcac ttttccctgt cacagcctct atcctgcgta caccggaggc taaggaacct
     2701 tcgctgacaa tataaaaact gcctatttca cctgtgtttc ttacatgggt ccctccgcag
     2761 agttcagcgc tgaagtcgct gatgctcact acccgtactt catcaccgta cttttcttca
     2821 aagagggctg tggcaccttc acgcatagcc tcttccctcg attttttctg cgtacacaca
     2881 tcaatacctt ccatgatctt ctcattgacg atatcttcga cccttgcaac ctctgactca
     2941 tccattgcct gaaaatgggt aaaatcgaac ctaagcctgt ctttatcaac gagggaacca
     3001 gactgcttta catggtcgcc aagtatttgc cgaagggcat actggagaag atgagtggcc
     3061 gtatgatttc tcgcaatgct ctttcttctt tcagtattga tggacaagtc cactacctgg
     3121 ttttttttca ggatgccctt ctcgacaact atggtgtgtg aaaagaggtc ttctttaatc
     3181 ttcttggctg cggttactcg tgccgtgccg ccagtccaat ctaccgtacc ttcatcttcc
     3241 acttgaccac cactctctgc ataaaaaggt gtttggtcaa aaaacgcctc cccagtttct
     3301 cctgccctga tctcatcgac catcgtatca ttcttgatga gggtgatgat tctggcttca
     3361 ggaatgcgaa ccatgtcata tccgagaaat tcactggaaa taccttgctt caagactgac
     3421 acatggactt gatcaaggcc ctcgcccttt attcttgaag aagttcgtga tcgttccttt
     3481 tgctcctcca gggaacgagc aaaaccatcc atatccatgg aaagcccgtc ttcctgggcc
     3541 atctctgtag taatatcaag gggaaatccg aaggtatcgt acagcttgta aatcatctcc
     3601 cctgacatta cggattcgcc ttttgatgat atctcatcta caaactcatc atagactttc
     3661 atgcccgtgc tgagggtctc aataaatctt tcctcttctc cctttatgac acggacaatg
     3721 taagggtgat tatttttgat gtcaggataa acatctccca tgatatctac cactgttgac
     3781 gaaaggtcag aaagaaactc ccgttcaata cctattttct tgccatatcg aagtgccctc
     3841 cgcagaatcc tccgcaatac atagccccgt ccgtcctttg aaggaaggac accgtcattg
     3901 ataacaaagg tagcgccccg tatgtgatct gaaatgacgc gcatggcaat atctgtcttt
     3961 tcattttcac cgtacacatt gtgggaaaca tcttcgagtc gcgtgataat gggccggaat
     4021 aggtctgtct cataattacc cagttttcct tgcataatag aggctatgcg ctccaaaccc
     4081 atacctgtgt cgattgatgg acgggggagt ttcgtcattt tgccatcccc agaccgatca
     4141 aactccataa agacaaggtt ccatatctca aggaaccgat cacagtcaca ccctactgca
     4201 caggatggtc ttttgcatcc cacgtgctct cccaggtcat agagaatctc cgagcatggc
     4261 ccgcatggtc cttcttcacc cattgaccag aaattgtcct tttctccgag ccttacgatc
     4321 ctgtcttccc tgataccgat gcttttccat atttttgctg cctcatcatc atctttgtaa
     4381 accgttatcc acatcttttt tttgtcaagt tgatagatat cccccaaaag cttccatgca
     4441 taagctattg catcttcttt gaaataatct ccaaaggaga agttaccaag catctcgaaa
     4501 aaagtgtggt gcctcagggt cttacccacg ttttcaagat cattatgttt gccaccggca
     4561 cgaacgcatt tctgacaggt tgtggctcta acatattcac gcttttcaat gccgagcaag
     4621 aggttcttga actgcaccat accggcattc acaaagagta agctggggtc cttatcagga
     4681 ataagagatg agcttgacac aatagtgtgg ccattttcgt taaaaaactc taagaatttc
     4741 tttctgattt cgtttgattt cactgttcag cctttttcat ttcgtaatgt tcgagtattt
     4801 tattttcaat ttctttcgca acttcctggt gcttcttaag atattccttg gtattttcct
     4861 tcccctggcc tatgcgcgtt tcaccgtagg cataccaggc gcctgctttc tctatgacgc
     4921 ctgcttctac acccagatct accatttctc cttcctttga aatcccttcc ccgaagataa
     4981 tatcgaattc aacctcttta aagggtgggg caagcttgtt ctttaccact ttcacccgcg
     5041 tcctgcttcc cactacatct tgaccgtctt tgatagcccc aattcgtctg atatcgagcc
     5101 ttaccgatga ataaaatttg agggcatttc ctcctgttgt tgtttcaggg ttgccaaaca
     5161 taatgcctat tttttgccgt atttgattta tgaatattac tgttgtaagt gatttgctta
     5221 tggtggctgt cagctttcgt aatgcctgtg acataagcct tgcctgcagt cccacatggg
     5281 catcacccat ttccccttct atctcagcgc gaggaacaag ggcagctaca gaatcgacga
     5341 ctattacatc tactgctccg ctccttacaa ggatttctgt gatttccagc gcctgctcgc
     5401 ctgtatcagg ctgagagata aggaggtcat ctgtgttcac cccaattctt tttgcatagc
     5461 ttatgtcaag ggcatgttct gcatcaacga aggccgccgt cccaccccct ttttgaatct
     5521 cgcttacaac ttccagggca agggttgtct ttcctgatgc ttccggacca tagatctcca
     5581 ctactctgcc ccggggaagc cctccaatgc caagggccat atccagtgcc atcgaaccag
     5641 tggaaactac cgggacctct tccagggctt tctcaccgag tttcataatt gcaccctttc
     5701 caaatagcct ttctatctgg gttaccgcca tgtctatcgc ctttgcttta ttgccggttt
     5761 cttctttcgc agccatacga cctccagttt ttaaattcag ctcacaactt taagctccaa
     5821 gctgatagct ctcagttaaa tccgtgacgc gtaacgcgta atcggtgatc tgtgatctct
     5881 gtgtttttaa tccaattccg atcacgcttc actgcatcac tgttttttaa gttttttcgt
     5941 cattcccgcc cgtctcgctt tttctgcttt taaaacctat ttcccacagt ggagaataga
     6001 tcgccccctg cggggtcagg gtacttctga acatgacaag ccggtcaaca ataaagccca
     6061 taccattcaa gtctggaatg tcgcgttcaa gaataggact ctgttccttc cttctcccaa
     6121 gggttatatg gggggtgtaa tctctttttt caccctcgaa cccaaggggc aaaagtgcaa
     6181 cttctatatc attaaatatc gcttttgcga tgtcaacccc cttttcaagg gtaaccacaa
     6241 ttactcgggc ccttctttta tttgggaaag catcaatgcc tttaatggtt gtctcaaaag
     6301 gctcaaactt atcctcaata cttaatagtc tcgttcttat gttttctacc atggcttcat
     6361 caagttcacc gaaaaatttt agggtaatat gctgtccttc gctctttacc cacctgacac
     6421 ccgtaatgcg tgatgccata atctttatga tcccttgaag gtattcctta acagagggag
     6481 aaatttccaa ggcaagaaaa accctcaact caacctaccc tccaaataat cgagcacaag
     6541 cttcagggcc tcttcagagg tttgctcctt gatggagatt ctgtcaccac tgaaaagaaa
     6601 tttgcgcaca acggtgctat ctttggatgc aagaccagca aatactgtac ctaccggttt
     6661 ttcaggggtt ccaccaccag gccctgcaac tccggtcacg gaaagagcaa tgtgtgcttt
     6721 tgtggtctct ctgagacctt ctgccatggc ctttgcaact tcagcactca cagccccatt
     6781 ttttgttaat accccttcag gaacaaagag atatagtact ttcgagtcgt tgctataagt
     6841 aataaatcca atatcgaagt agtctgatgc accctgaata ttggtaattc tgtttgctat
     6901 atgaccaccc gtacaagatt ctgcaaccgc tatcttcatg cgtctttccc tgaggattct
     6961 ccctattttt gcttctatca tgtcagtatc ctctctccaa tgattataat aatatttgca
     7021 aaaactccag ctacaacatc atcccctaca ataccgtatc cttgcctgag tgtctctacc
     7081 cgtttgatgg ggtaaggttt tttaatatca aaaaaccgaa agagaacaaa tcctgctaac
     7141 aggctgaaaa gtgtaatatt gtggccagcc atagtgacaa acataccagc cacttcatcg
     7201 atgatgatat atcggggatc tttcccctca aaagcaaggt tattcaatac aatcagggtg
     7261 acaactataa aaatgaagac gaagagagga tgagcaaaaa ctgagggcaa aaaatagagg
     7321 agaaggcacg caagggctga tccatatgtt ccgggcgcat aaggtaaata accaattggg
     7381 ccaagcgtca caaaggtgtg aaagacatta ttttttgtcg ccgtagacct ctttgtcatc
     7441 aaaaaccttt gtgccaacta tattccaacc atctttccag accctgtaga agcaactcca
     7501 aaatcctgtg tggcaggcgg caaccttctg atctaccata ataagcaggg tatcgttatc
     7561 gcaatcaata taggttgatt ttacttcctg gaaatgcccc gaagtctcac ctttcagcca
     7621 aagtttcttt ctcgacctgg agtaataatg ggctttgcct gtttttttag tcaactcata
     7681 tgcgtcttga ttcatgtatg caaccatgag cacatctttc gtatttatat cctgaacaac
     7741 aacaggaaca agcccctttt catcccactt tatttgattc attgtatctt ccatccctta
     7801 ctttgcgttt tttatactcg acatactgaa atgatgttca tattgtatct ccacttctgt
     7861 gtcatgagat acaattatat cgagaaaata gtgttttttg ttcatttgca cttcatgttc
     7921 ctttgcatta agaagtgatc ctttaatcag catattgtat ggcttcttcc acctttatca
     7981 ggccttcata gaccgcctta ccaagtattg aagcccatac tccaagatct ctgagtttct
     8041 tcacattgtc aatatgagta acgccaccac ttgcaataag agggatgtct gtcaactgca
     8101 tcatcttttt catgccatcg tagtccggtc ctgcaagcat accatcctta gcaacatttg
     8161 tgcagagaat tgccataatg ccaacctgct tcgcctcatt cagtatttcc tgaacatccc
     8221 tatttatggc gctcttccat cccctgacca taggttttcc atcgagcaca tccagaccaa
     8281 gaacaatatt gttgtatgtg gcaagattcg caaaaaagag ggcatcttca agggcctgcg
     8341 ttccaactat aatgccattg atgcctgcag aggtatagtc tctgatgtcc tcttcacgcc
     8401 ttatgccacc ccctacctcc atatagcccg gcactttctc gcgaatcttc aggataatct
     8461 ctgtgttaac acgattgcct gttcgtgcgc catcaagatc tatgatgtgg aaatccctgg
     8521 caccccgttg cacaagttct tcgatcttgt caactggatt tttactgtag acagtgactt
     8581 tcgaaaaatc gcctttgact aaacggacgc attcgccatc tatgaggtcc atggcgaaca
     8641 gagtcttcat ctatagtcct caagaattat atccagcccc cggctgatgg catcccggca
     8701 aagttcttca acggtgagcc attttgcgcc tcttctgaga aactttccta aattaaatag
     8761 attttctgac aaagcttctt gatcctcatc aaaggtaaac actcttttca gcacgttgtt
     8821 ttctataaga tgcatatgga agccagcacc agcaggtttg tcaacaccaa gattgccacc
     8881 tatcctctct tcatatctat agatcaggag atggacatag ttaccgttgt ttttatcctt
     8941 cccataccct ccgattcgtt ctgaaaaact ttctcgcaaa atgttattga tcgttgcctc
     9001 tgcaccctta gggatattgc caaccttcat atatctacca caaacactac attttttgat
     9061 ctcgccggga gaaccgctca tcttctccac aacaataccc aggtctccaa tagcatggac
     9121 attattggtt ccagcaaatg acaagagaag aaaaactgtc gcgaatatca gtggtgcctt
     9181 ttcacggaac atttttattg gtatcatagg tatcattcta cctaaaaaag tatggaaaga
     9241 aaaggataaa aagccttcac ctcattgcat caatgcaggt attgtaaagc agaatgagat
     9301 taagtccaat caaaagaccg gtactgacat aagtcaccat gttcatccag tgagaattcc
     9361 ggtactcacc cataagttct tcattgttca ccagcatgag catgaaaatc aggatcaagg
     9421 gcagaagcaa gcaatttatg atttgactaa accacatgac ctttatgaga ggtatatttg
     9481 gaataagaat gattgaagct cctaagataa caatgatcgt aaaaatagta ttaaactggg
     9541 gtgcttcttt gaaacgtttt cctgtgcctg tttcccagcc catggcttcg catatgtaat
     9601 atgtggtggc aaggggcaga atgcatgcag aaaaaagcga ggcatttgca agccctatgg
     9661 caaatagaat gtaggcatac ttgccagcaa gtggttttaa tgacagggct gcatcttcag
     9721 ctgtgtgtat atgtatgcct gctggaaaaa gagtggctcc gcatgcgaca attataaaaa
     9781 aggccactac acccatcatg atgcctcctc ctatgacatc aactttggaa gcccatctat
     9841 cctgttcatt cagacctttc tctacaacac cggcctgcag ataaaactgc tgccatggtg
     9901 tgatggaagt acctatgata cttattatca tgatcagata gccggaactc cattcaatgc
     9961 tcggagtgac tgtcttctgc atcacttccc cccagtcagg tttcgcgaga aatgctgaaa
    10021 ttatgtatgt actgtatata agacaaacac tgagaacgat tttctcaaag attgagtagt
    10081 tccatcgcgt cacaagaaac cagacgaaaa aagctccgat cggcactgat atgtatttgc
    10141 tggcaccgaa gagctccatg ctcgcagccc atcctgagaa ttcagccatt gtgttgccaa
    10201 cgttcgtaac aaagagggaa agcatgaccc agaaggccac cctgatacca ctattctccc
    10261 gtatgagatc tgccagacct ttgccagtta ccattcccat tcttgcagac atctcctgat
    10321 agacgatcag gagaaataca gtgggaatca gggtccagag aagattgtaa ccataccgtg
    10381 ctcctgctat tgaataaatc gcaatccctc ctgcatcatt gttgatattt gacgtgataa
    10441 ttgcaggacc cagaagagaa agaagaatta tgaacttttt cattcgtgcc atactacctt
    10501 aattaacacg atattttttt tcgttcaatt tatttcaata aaatggttcc atttttttaa
    10561 accatcgtaa ctacctcggc ccaacaaagt tataactcat attggatttc ccaacacagc
    10621 aatttagctc aacttataaa tcagccctga aagaggtttt tatgaaaatt atgaaaactt
    10681 ttcttgatta aatatctgat atgtatacaa taaatattta aagcagttaa aagctaacag
    10741 aagtaatgca atggcggcaa aatcaagctg gcggcaaaac catcagggaa aattgccact
    10801 aaaccagcgg agaagaacca gtcgcgtaag agggaagcaa gtatacgtgt agcttatgca
    10861 gaatagtcgt atcagttggc actgcttata gatgtattga accatgctat ctcattttct
    10921 gttcaaaacc aatgaaattg agaaatgcat cacattctgg gtcagaatcg aggacaagac
    10981 cggttgccct cttctgatga atttaagtga acaaatgaat tgatgtgtgg tattttaata
    11041 actagctgca gcacctgtaa tcagcactat tgaaatacag cctgttttgt caaagaaaat
    11101 gatgaaaagt aactgcggac attacatttt ttgtttcatg aagccatggg tgaaaatgct
    11161 ggttttctgc attctttcct tttttgtcag ttttccctat gccctcgcca taaacgtagc
    11221 gtctgttgaa aaatttttag ataacgctgc ctttgacgca tgggacccca attccaacga
    11281 ccttctcttt ttaagaaaag atgaaaaggg cgttttgcag attttcaaag ttcgagaaaa
    11341 ttctataaat cctgaatcag acaaggtatg tatcagttgt aatgctcaac gggcggttgg
    11401 tttcaagcta tccacagttc ctttaattca taagggcgcc tcagactggc acccttccgg
    11461 cgagtggttc attacccagg gagaaattcc ggacaacatt agctggaaac aggaaaaacg
    11521 gatacctggc gcaagattga atgcagagcc ggaaacagga tggtggaata atctttttct
    11581 cgtcagtaaa gacgggaaga tatggatcga acttaccaaa ttcacaaatc atgatatcaa
    11641 cagcggcatc ctttctccaa cgttttcaaa agatggaaaa atgatcgcct gggctgagcg
    11701 cattggaggc tccagaccgt ttgatcaata tccaaatgga cgctggctcc ttaagaccgc
    11761 aaagatccac gtgaatgggg aagatacgag aatctacgat atcaaagtac accctccaca
    11821 ggatggtgct acattcaaac ctcaggggtg gtcgtcagat ggaaaactac ttttagcaac
    11881 agatatagga tacggtgaac tgccctatcc tggttacaga gctgatattt atgaagtcag
    11941 attcgatcca gatggtacca taaaaaacat gaaaaacctt accgggtcaa aagacttcct
    12001 tgagcactat gcctcctatt cgccggatga aaaattgatt gctttcacaa caaacaactt
    12061 cgacccacaa tatgagaaac gcatgaacga tgcatggaaa aaggacggac cgcgcttcag
    12121 tcactttatc gtgcgaaacc ttttcaccga tctctttctc atggaccggg atggcaaaat
    12181 tgtcaagcgc atcaccaact ttgttgacca ggagtggaaa ggaacacacc caatcgtagc
    12241 aaagaacgca tggagtaaag acgggaagac aatcatcctc ggtctggtcc ttcgttctaa
    12301 tataacaggg aaaaaggaag gtgaaatgat ttacaggatt cgcctggaat gagcctctca
    12361 ccagaacatc catcacctac taccgattgt ctgtggtctt taacctccaa tggactgaga
    12421 acctgtggtt agtcctgctc catcaggttg accttcttca ttcccaattg cgcccctcca
    12481 taagcactct catcatctgt ggtgttcatc cgccagcttc tttctcaccg gatatgcgaa
    12541 agggatttcc tcctctttgc tgtgatgacg tcggtctgca tgaccatgca tggagtcctt
    12601 aaatgcattt gtaagccctt catcaaccaa tcatgttttt acgatgatgg gaacttccac
    12661 atcaataatg gatatcattt tttcaataac actgtattcc actattcata gtgctgcgga
    12721 tatcatacgg caacatctca cgtatgcact cccgtttatg ctttatgata ccagccactt
    12781 aaactgcaac cggcggtgct gccgggtcat atttgctcat aagaaaaatg gctttagtgg
    12841 gacatgacag catacaggcc ccatcaccaa tacagttttg cggcctcacc ggttttgcaa
    12901 caccatccac aatgtcgaga acgtttggac caagcttttt gcaaagatta acgcaattcc
    12961 tgcacccaag acacctcacg ctgttaatct ccgggatatt tcctttaata aatgtacgcc
    13021 caaaggcctc ggcggtttct gtctgcactg tttttcctgg catggtataa tcctcctttt
    13081 atttcatatt tcttagtgta atctgccagc ccggttatgc ccacaacagc aggttcagca
    13141 tccacaacct gagcgacgtg atgggagtga tcagttctct tcacaacgtc tgaggcaatg
    13201 cctcccatga actgcttacc cataccagac cctccataag acacaattgt ccatttatct
    13261 tcaaggtgga aaggaaatcc tcaacactca tttcacaagt agtgtctcac acttcgcgcc
    13321 ttttagaaca ttcgagctta cactcccgag gaagtatttt gcaatggctg atcttccgcg
    13381 ggacgcaatg acgatgagat caactccatt ctgctcctga aattttagga tttcctcgta
    13441 gggaatcccc tgcaccactt cagctgaagt ctttacctcc ccagacagag gaaacttggt
    13501 ctgtatctga tggaggctat tttttgcgtt ttccattacg cgtttctcca tttttgtaac
    13561 ttcatcttca gtcaagcaat aatcgaccgt gcacgcctgg atgtcctggt cgataacatg
    13621 gaggagaaca acttcagagt cgttctgttt tgcaacatcg agtgcccgtt caaatgctct
    13681 gtttgagtgc tctgaaaaat cagtaggtac taaaattttt tttggcttca ccatgaaata
    13741 cctccttgtt ctacccggta atcttcttct gaaatatccc tgtttttcac ttaaccaata
    13801 gcacctcacc ctttgcgtgt ttcagtaccc tgctcgccac atttcccatg aagaaagcct
    13861 taattgccga acgtccgtgc ggagatatga caataagatc agcacctttc tcttcctgta
    13921 gctttataat ctcttcgtag ggaattccat ttcttgcttc gatggaaacc tttacctctt
    13981 tggatgatgg gaatttgtcc agttgttttt gcatgttttc ggatgctgcc tgaaacaccc
    14041 tttttttgac agcgtcaatt tcatccgctg taaagcaata gtcaacaaca caggtctgaa
    14101 aatcttcgtg tattacatga aaaagaataa tctcggagcc ctcttctttg gcaatatcga
    14161 tcgccttctc aagtgcccta tctgcatgat ctgagaaatc tgttggtaca agtatttttt
    14221 taggcttaaa cattgtacac ctccttgatg tagttggact gcattaccgc tgtttttttt
    14281 aaccttgcat gacccctgat gtcaatagtg gactccaaga gaggcagaaa catacgtgca
    14341 gacgtccctt cctcttttct gcttattatt ttannnnnnn nnnnnnnnnn nnnnnnnnnn
    14401 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    14461 nnnnnnnnnn nnngagaggc agaaacatac gtgcagacgt cccttcctct tttctgctta
    14521 ttattttaaa gcaacctttg tgggcctgca caatttgatt gacaagtgta aggccaagcc
    14581 cgtgaccagt ccctgttggt tttgttgtaa agaaaggttc aaagatatgc gatttcagtt
    14641 gggggtctat ccccttgcca gtgtcaacaa cactgagtaa tgcacattcg acaattttat
    14701 gttgagttga atcaggactg ttcattctca cctttacctt ccgcgtgacc agggcaatgg
    14761 ttccaccgaa aggaagggcg tcccttccat tcaagatcaa ctctccaaca gcctgtttta
    14821 taaatctcat atcccccttc attggcaaag aatcttcagc tatatcaact tggattgtaa
    14881 tatcatctcg cataattgga gagataaaag gaacgatacc cctgagaatg tcattgagat
    14941 caaagtcttc cattttcatg ggcatctttc cattgagaat catgagcgtg taaataagat
    15001 ttacaccacg ggcatgcgaa tcctggatgc gaccgaggta aaactgttcc agcgcctttt
    15061 tttctatttc ttcttcaaga actgccccgt gctgcattac tgatttgaaa ggcccgacag
    15121 ctggcaaaac cacctgttca acagctcttg atgtctttgg atcgctgctg aaaccatttc
    15181 tttcatcctg ctttttagtc tggataattc ctgctctatt ccacaggctg gctaacgccc
    15241 agtcaataga aacatttgca ttgacagaga ccacacaatg ccccgtgctg atttcactcc
    15301 aatagccttt ggacatgatt gcctccccaa acttaagatt gaccttggtc tttctttttc
    15361 aagaatcgcg ccactcatgt gaggtagttg aattattttt tattcccctg attacagtga
    15421 gttagcattt gtggaattga gatttgaaac ataataacag gagagttgtc ctgaaaaacg
    15481 agactaagtc ctgcaggaca agacatgaac gccaccgggc cctttttcag ctaatatcta
    15541 tttaaggttt caccttaatg agtctatgct ttttgagtag agcataaaga cgtgaacgtg
    15601 acaatcctga aatccgacag atctccttga cattgttctt cgttcgggca atcagttcca
    15661 ccagatattg ccgttcagcc tcatcgatga tatcccccct gaatgaactc aacgtggtaa
    15721 attcctcgat cttccctcca cccggaagaa caggctcgac tggttttttc gtttctttca
    15781 gggcagcatg ggccagcttc acacgcaggt aagtcggtag atgtttcgga taaagggtcg
    15841 gctcatggag agcactgcaa actgcagtat ccatggcatt ggcaaattct ctgacatttc
    15901 ctaaccattc atacccagta agcgcgtcaa agaattctgg tgtaacgcct ttcatcgcaa
    15961 ccccgtatcc ttcgcagaac tttctaagat agtgtatcgt cagctccttg atatcttcct
//
LOCUS       DAOOMC010000065        15880 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdus sp. isolate AMR_MDS_2685
            NODE_3899_length_15880_cov_6.574408, whole genome shotgun sequence.
ACCESSION   DAOOMC010000065 DAOOMC010000000
VERSION     DAOOMC010000065.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864760
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdus sp. (wastewater metagenome)
  ORGANISM  Syntrophorhabdus sp.
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae; Syntrophorhabdus.
REFERENCE   1  (bases 1 to 15880)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15880)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,729
            Genes (coding)                    :: 2,708
            CDSs (with protein)               :: 2,708
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 7 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15880
                     /organism="Syntrophorhabdus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3899_length_15880_cov_6.574408"
                     /isolate="AMR_MDS_2685"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2699756"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..205
                     /locus_tag="PK125_07955"
     CDS             <1..205
                     /locus_tag="PK125_07955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB38079.1"
                     /translation="AKHTDPCIINKFQLAPTNWWRRVIIPSVYSTRLKMCHLASPLHS
                     LACLIWRKAIHFPASLVISIPTI"
     gene            339..1097
                     /locus_tag="PK125_07960"
     CDS             339..1097
                     /locus_tag="PK125_07960"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HPB38080.1"
                     /translation="MMQELVIKSYGDLGFHKTIGELIKEHSENKEDIREVAKRLVDLT
                     RVHDILDLGCGYGWFEEALPSGFRSIVGIDCLDANEQGFLSVARKIATHSAFKRVLLP
                     APIDMEADTFDLIISAYSLYFFPDVLPEVHRLLRPTGVFLIITHSASMMEEGEEFFDF
                     SNVRQVINNFSAENGEHILRQHFELVTATDYINSLVFVKEEKGDLAKYIDFKREFISK
                     DVSPVVVKAKMIHELETRGMMKFNKNDRIFVARK"
     gene            1094..2098
                     /locus_tag="PK125_07965"
     CDS             1094..2098
                     /locus_tag="PK125_07965"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HPB38081.1"
                     /translation="MKTKAFCNFCGKELVTDMLEGRERLVCDSCKEIHYENPLPVAAA
                     VLVNREREILLVKRAREPSKDMWCFPIGFAEMGESIEDAALRELHEETGVEGRIIQLV
                     DVCSETNKFYGELLIVTFEVEKMGGKEQAGDDALDCRYFPIKNLPRLAFPSQQRVVEK
                     LIDLKKDLWSMHDSMESFVEGTFGNKIVYPGKLLSDELVNAVENNSTKIIELWLNDIA
                     TNPSTKAYHTFDRKELYNRAMFIIGQFEVWLKGQKGETELRAFYNELGFQRKKDGIPV
                     EDLVSSLSLLKKHIWMFTYSFGVWEKAVDIYRMFELGERLVYFFDKASYYTVMGYNQK
                     "
     gene            2162..3502
                     /locus_tag="PK125_07970"
     CDS             2162..3502
                     /locus_tag="PK125_07970"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPB38082.1"
                     /translation="MIVTTIGSFLMPFALSAVNVALPSIGRELNMHAVSLSWVALAFT
                     LSAGMFLVPCGKLADIWGQTRMFIAGNWVFTVASFLLTIISTPLMLIAFRAMQGLGAA
                     MLYGTGLAILVAAYPLEERGRVLGINVASVYLGLSIGPFLGGVLTQNIGWRSVFFLNV
                     PFGLLNTFMAIRWIPRRPGERKSGGFDIIGSCIYGAMLFLLLYGFSVIPSIAAVAFIF
                     GGMLGIVLFVWREKRIKYPVLDVGLFMKNRSFTLSNIAALINYAATFAVAFLLSFYLQ
                     HIKGFAPQKAGLVLVCQPVVQAVLSPLAGRLSDRIEPRVLASIGMAIAALCLFILTSF
                     TPGTSLVFIISTLGLFGVGFALFSSPNVNAIMSSVEDRLHGVASSMVATVRTLGQMFS
                     MGIAMLTFALFMGQVEISPALYPLLLKAIKTACTIFGFLCVVGIFASMARGNLR"
     gene            3555..4388
                     /gene="nudC"
                     /locus_tag="PK125_07975"
     CDS             3555..4388
                     /gene="nudC"
                     /locus_tag="PK125_07975"
                     /EC_number="3.6.1.22"
                     /inference="COORDINATES: protein motif:HMM:NF001299.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000210 - NAD+ diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) diphosphatase"
                     /protein_id="HPB38083.1"
                     /translation="MEGLFESSVKGPVEKTHPAFWFVFKEKRMLCVKQEAENNPLFVV
                     DIRDIGIHPDRELYLGLLDGFHCYVAEVSEPTRAPEGMAFHGLWSIHGSMDENLFKVA
                     FRAIHTLEWDRTDQYCSFCGSRNRQKVQERAKECPQCGRVSFPRISPAIIVLVEHDGK
                     ALLARSPRFKEGLFSVLAGFVEPGETLEDAVSREVKEEAGIDVKNIRYFGSQPWPFPD
                     SLMIGFTAEYAGGDIKIDNDEILDARWFSAEDMPEIPGRISISRALIDWFLDRQKGKK
                     T"
     gene            4667..4987
                     /locus_tag="PK125_07980"
     CDS             4667..4987
                     /locus_tag="PK125_07980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB38084.1"
                     /translation="MEKKLIKEHRKQTEPQQTIEVPANLLKELYQITAFCSGDCPARE
                     GERPRPPDGTKKGDFEAEKALKSTQWKKEQEEHEAAKKERNAQVKKERTREAVRGEVK
                     TPAI"
     gene            5225..6496
                     /locus_tag="PK125_07985"
     CDS             5225..6496
                     /locus_tag="PK125_07985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease Q family protein"
                     /protein_id="HPB38085.1"
                     /translation="MRFIADLHIHSKYSRATSRDMSPEVIWKWAQIKGIGVVGTGDFT
                     HPGWLKELSEKLEPAGEGLFKLKKEHELNDVPDSCRAEVSFILSSEISSIYRKNGRTR
                     KVHSLILVPGFSDAARINLSLSRIGNLNADGRPILGLDALDLLKIVKEASPSAIFIPA
                     HAWTPHFSVFGAASGFNSLEECFENYAPHIKAIETGLSSDPPMNWRVSALDTINLISN
                     SDAHSPAKMGREANIFDTDMSYNSIASAIETRQGFLGTIEFFPEEGKYHYDGHRACGV
                     SLSPEETIQNNYLCPKCGRRVTVGVLHRVEKLADRPSGYKPHNATPYFPLIPLTELIA
                     CTLGLGVTSKAVEMEYHRLIVTFGNEFSILMDAPITRNTQRNWGLIGEAIRRMRSGNI
                     NVSSGYDGQYGKINIFNVGEREEIKRQATLF"
     gene            6534..7472
                     /locus_tag="PK125_07990"
     CDS             6534..7472
                     /locus_tag="PK125_07990"
                     /inference="COORDINATES: protein motif:HMM:NF012335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HPB38086.1"
                     /translation="MRFSRNHPVPEPGDGEALVKVCLAGICNTDLEIIKGYMRFQGVI
                     GHEFVGVVEKTWENQRLFIGKRVVGDINCGCGACLYCKAGLQRHCPSRTTLGIAGKNG
                     AMAEYITLPVHNLFLVPENVKNEEAVFVEPLAAAYEILEQIHLRPTDRVLVLGDGKLG
                     LLVALVLDLCGPEVALAGKHEDKLAIARGQGVCTVALESLTGDKAFDIVVDATGSPKG
                     LEAALRFVKPRGTIVLKTTAAEGITMNLAPVVVNEVKIIGSRCGPFGPALMCLAQKRI
                     KVGPLISGVYGFSHAGEAFTKAQDKRSLKVLIDFSK"
     gene            complement(7566..10241)
                     /locus_tag="PK125_07995"
     CDS             complement(7566..10241)
                     /locus_tag="PK125_07995"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HPB38087.1"
                     /translation="MTAADYSEDERYKELADLLPQSVFEIDDEGKLTYVNKCALEKFG
                     YTYDEFKNGLSSLQMVVHEQRQLAMENIKMSILGKSPGFEYTMQRKDGSTFPAIVNSS
                     PIIRNGLRVGLRGIIVDITERKKMENALLQSEQLLRSLVDNMLDATLILDWNGNVLFA
                     NNAAARLVDIEVPVNVTEFNIRDFLHPEHEQTAFEHLDRVNNDKGGFLWQYKIITKKG
                     REKWVESLGTKIQFNGLSANIVNLRDVTERKRAEQALKETVDKYRNIFENAVEGIFQS
                     TPEGRFIDVNPALAHMMGFQTPDEMIRNFTDIAHQHYVNPEGRSEYRALLDKDGVVKG
                     FEAEVYRKDKKSIWISINARAVLNKSGDVLYYEGTVENITERKQYEDSLKSLEELESS
                     ILSAIPHAVIGLQARTIIFANESVEAVFGWTPKELIGRNTRILYRSDEEYEEIGKRFY
                     PVLEKQRFHREMFDCRRKDGKDILCMVSSARIGNDLTDKKIVVIYEDMTERKRTEMEK
                     SHLEAQLRQAQKMEAIGQLAGGVAHDFNNILTAVIGYSNLLKAKMAQDDPLRTYAEHI
                     LASAGKAVNLTQSLLAFGRKQVIELKPCKINTLIKGVEKLLARLLTEDIELRTFISES
                     DPTILADPTQIDQILINLATNARDAMPNGGSMKIEVKLDRIDHAFIESYGYGEIGKYA
                     LISVSDTGLGMDSTTKEKIFDPFFTTKEVGKGTGLGLSIVYGIVKQHNGYINVKSTPG
                     QGTTFHIYLPVVKRPEEEIKPEVIPVRGGSETILIGEDNATVRQLTKEVLEQFGYTVI
                     EARDGEDAIRQFEQSVQTVDLVILDVVMPKKNGREVYEVIKTIKPEVKVFFMSGYTAD
                     ILTDKGMGEEKLDYTSKPIAPQELLLKVRTVLDGR"
     gene            10390..11163
                     /locus_tag="PK125_08000"
     CDS             10390..11163
                     /locus_tag="PK125_08000"
                     /inference="COORDINATES: protein motif:HMM:NF038402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helical backbone metal receptor"
                     /protein_id="HPB38088.1"
                     /translation="MWLILVLFLIPFSVHGYDRIISLSPQITESLYLIEAAKELVGVT
                     EFCKKPAEATKKQKVGTPLRPDVEKIVSMRPDIVFGTREGNSPLVMARLERLNIKIHY
                     FNRPKTFADLMDNFLTLSKCVGKEEKGKQIVNNAREALSKLKNEKGYRVLWQVGADPL
                     VVASNNSFANDIIVLAGGSNIIESELPYPRINTEEVILKNPNVIVLMDMGYNVQLEME
                     RWKRYFNKVKFVILDAYSVGSPTPVSFVNAVKTLAELFP"
     gene            11160..12137
                     /locus_tag="PK125_08005"
     CDS             11160..12137
                     /locus_tag="PK125_08005"
                     /inference="COORDINATES: protein motif:HMM:NF013219.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HPB38089.1"
                     /translation="MRRKQKSVVVLTILVCLLLVSILLSLAYGKGIGVMFHSFFNNDG
                     FGLILRKIRIPRTIIAFLVGGSLGVCGVTLQSILRNPLAEPYTLGIAGGASFGIALST
                     IIGFQRIFGAFANPLFGFGGAMLSVAIVFALSKKRFFDPNSMVLFGVVVSLVFSSFVF
                     FLFSLLDPDKMQTTLMWLMGDLSSLDIAFIPMYIPAFLIPTSILFFFGKELDILSLGR
                     EKAQYLGVNPPRFYKGLFLLSSLLAGLCVSASGIIGFVGLIVPHILRNIIGAGHTVLL
                     IASYIGGAFFLILSDVFSRYLLYPVELPVGVVTGILGGFILLFLLIRRK"
     gene            12137..12934
                     /locus_tag="PK125_08010"
     CDS             12137..12934
                     /locus_tag="PK125_08010"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPB38090.1"
                     /translation="MGDALFSVHGVVFNYDRHTAIDDITFTVNPGEFMGIIGPNGAGK
                     STVIRLLAGFLKPNKGFVAFSGKNLDKYGKRELAQNIATLPQATETPFSFTVEEFILM
                     GRYPYAQKNWLYEKKEEEFVEYIIDTMEISHLRGRTIDTLSEGERQKVFLSQCLAQDP
                     KALLLDEPVSHLDIRHQMQTFDVLEKLHSEGLTIIMVLHDLNLAAEFCSRLMLISKGR
                     INADGDPKTVLTFQNIEEAYDTVVIVKENPLSGKPFVVPVSRKYLGK"
     gene            complement(13003..14100)
                     /gene="dnaJ"
                     /locus_tag="PK125_08015"
     CDS             complement(13003..14100)
                     /gene="dnaJ"
                     /locus_tag="PK125_08015"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF008035.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HPB38091.1"
                     /translation="MRKDYYQILNVHREASDEEIKKAYRKLALKYHPDRNPHNKEAEE
                     RFKEISEAYEVLSDQEKRFRYERFGSADDSGSIFDFGFRGNFDDIFNDLFSDFFGGQR
                     QREKKGEDLRYNLQIEFEEAVFGVEKEIEVPKEERCQSCNGSRVEPGHKPAVCKACSG
                     RGQVRQSHGFFTINRTCEYCNGEGFIIKDPCKTCKGRGSIKTKKKLKIKVPPGVDNGA
                     RLKLRGEGAQGFAATVPGDLYVVLHVKEHPIFEREGDDIAVQVDVNFPLLCLGGDITV
                     PTVEGETVLNIPPGTQPGKIFRIKGLGVTKSNGYGRGDELVYLNVGIPTELTERQRHL
                     LQELAQEFNGIVNPSRRGIKEKFKEFFDWKE"
     gene            complement(14118..14690)
                     /gene="grpE"
                     /locus_tag="PK125_08020"
     CDS             complement(14118..14690)
                     /gene="grpE"
                     /locus_tag="PK125_08020"
                     /inference="COORDINATES: protein motif:HMM:NF010738.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - chaperone binding [Evidence
                     IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HPB38092.1"
                     /translation="MEEKEEKNSEKKTKEKGEPHEDHKKKKKKDDVAEELQKILEEKE
                     ESFKELNSKYLYLQADFENFKKIKAREKQDLLKFGNEVLIKELLPVIDNLERAIDHAE
                     KYQDDQGIVEGVKITLNEFLRVLERSGVERIDAMGKQFDPNFHEAFFQEETENMEPNI
                     VISELQKGYTLNGRLLRPSRVSVSKKPEAQ"
     gene            complement(14702..15733)
                     /gene="hrcA"
                     /locus_tag="PK125_08025"
     CDS             complement(14702..15733)
                     /gene="hrcA"
                     /locus_tag="PK125_08025"
                     /inference="COORDINATES: protein motif:HMM:TIGR00331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HPB38093.1"
                     /translation="MDVLNEREKMVLELIMERYINSAEPISSGTIAKTIKDRLSAATI
                     RNVMADLEEQGFLYKPHVVAGRIPTYKAYRYYVNSLSLLKLPGKKELIALETMIKPHY
                     SYIEEIMEDASKILASLSKYTSIVVEPKVDTMLFKEVEFVKLSSRTVLAVFVTSSGMV
                     HTRLVNTDEEFDVAMLHSMKAYMNEKFGGMPFYALKQGILEDIKKDRENYSRLLAKIK
                     QTLETIIDEEDVREIYLEGASKIIGVPGFADVERLKGLFQAFEKKEKLLELIDSCLKE
                     EGIHVIIGGESDIKEMSDMSIITSTYRVGEQSFGVLGVIGPIRMNYSKIIPLVTYAAN
                     TVTNILTTV"
BASE COUNT         4181 a         3586 c         3486 g         4627 t
ORIGIN      
        1 ggccaaacac accgacccgt gtatcatcaa taaattccag ttggcgccaa ctaactggtg
       61 gcggcgtgta atcatcccct cagtctactc aacccggttg aaaatgtgcc accttgcatc
      121 tcccttgcat tctttagcct gtctcatctg gcgaaaagcc attcactttc ctgcgagcct
      181 tgtcattagt ataccaacaa tttaacaggg tatccagcta cattgattgc aaggccacct
      241 ccttgtggta tttattttca atacccgtaa cctgttactg atggtatgat ctttaatttg
      301 aggtttacaa aaccccggcc ttacgatata aatgaagtat gatgcaagaa ctggtaataa
      361 agagctatgg ggacctcggg tttcacaaaa ccatagggga actcataaaa gagcattctg
      421 aaaacaagga ggatatcaga gaggtagcca agagacttgt tgatctcaca agggttcatg
      481 atattcttga ccttggatgc ggctacggct ggtttgaaga agctctgccc agtggattca
      541 ggtctatcgt aggcatagac tgtctcgacg caaatgagca aggatttttg tcagttgcaa
      601 ggaaaattgc tacgcattca gccttcaagc gagttcttct tcctgcaccg attgacatgg
      661 aagcagacac atttgatcta atcatttcgg cttactccct ttattttttt ccagatgtgt
      721 tacctgaggt tcacaggctg ctgcgaccga caggtgtctt tttaatcatc actcacagcg
      781 cctccatgat ggaagagggg gaggaattct tcgatttttc aaacgtccgg caggtaatca
      841 acaacttttc agctgaaaac ggagaacata ttctcagaca acactttgag cttgttacag
      901 caacagatta tattaattcc ctcgtttttg taaaggaaga gaagggtgat cttgcaaaat
      961 atattgattt taagagagag tttatatcca aagacgtgag ccctgttgtg gtgaaagcaa
     1021 agatgatcca cgagcttgaa acgaggggga tgatgaagtt caataaaaat gatagaatat
     1081 ttgtagcacg aaagtgaaaa ccaaagcatt ctgtaatttt tgtggtaagg aactcgtcac
     1141 cgacatgctt gaaggcagag aacgtctggt ctgtgacagt tgtaaggaaa tccactacga
     1201 aaatcccctg cctgtagcgg cagcagttct cgtgaatcgc gaacgagaaa ttcttctcgt
     1261 aaaaagagcc agagagccct ctaaagatat gtggtgtttt cctattggct ttgcggaaat
     1321 gggagaaagc atagaggatg ctgccttacg agaactgcac gaggagactg gagttgaagg
     1381 aagaatcatt caactggtgg atgtctgttc tgaaaccaat aagttttacg gcgagctgtt
     1441 aatcgtcaca tttgaggttg aaaagatggg tggcaaggag caggcaggag atgacgccct
     1501 tgactgtcgt tattttccca tcaaaaatct tcctagattg gctttccctt cgcagcagag
     1561 ggtagtagaa aaattgatcg acctcaagaa agacctgtgg agtatgcatg attccatgga
     1621 gtcatttgtg gagggtacat ttgggaacaa gattgtgtac cccggaaagc ttctatcgga
     1681 tgaactggtc aatgcagtag aaaacaactc gacaaagatc atcgaactct ggctgaatga
     1741 tattgccaca aacccatcga caaaagctta ccacacgttc gacaggaaag agctttataa
     1801 ccgagcaatg tttattatag gtcaatttga agtatggctg aaaggacaga aaggggagac
     1861 ggagttgaga gctttttaca atgaactggg cttccagagg aaaaaggatg gtataccagt
     1921 tgaggatctt gtgagctccc tgagtcttct gaagaagcac atctggatgt tcacatactc
     1981 cttcggtgtg tgggagaagg ccgtggatat ctaccgcatg tttgagctcg gagaacgact
     2041 cgtttatttc ttcgataaag cctcatacta cacggttatg gggtataatc agaagtaaga
     2101 aacacgacaa gacataaaac aaatgaaagg acctaaagcg gccaaggggg gaagaccagt
     2161 attgattgtt acgacaatcg gatccttcct catgcccttt gctctctcgg cggtgaatgt
     2221 agcccttccc tcgatcggga gagaactgaa catgcacgca gtgtcgctga gctgggtagc
     2281 ccttgccttc acattgtccg ccggcatgtt tcttgttcct tgtggaaaat tagctgatat
     2341 atggggccag acaaggatgt tcattgcagg gaactgggtc tttaccgttg catcgttctt
     2401 gctaaccatt atctcgacac ctttaatgct catcgctttc cgtgccatgc aaggccttgg
     2461 agcagcaatg ctttacggaa caggccttgc cattcttgtt gctgcctatc ctctcgagga
     2521 gcgggggcgc gtactgggca tcaacgttgc atctgtctat ctaggccttt ccatcgggcc
     2581 ttttctggga ggagtgttaa cacagaacat tggctggcga agtgttttct ttctgaacgt
     2641 tccctttggc ctcctgaaca cctttatggc cataaggtgg attccaagac gcccaggcga
     2701 aagaaagagc ggagggtttg atattattgg atcatgtatc tatggtgcaa tgcttttttt
     2761 gcttttgtac ggattctctg ttattccaag tatcgcagcc gtggccttta tttttggcgg
     2821 gatgcttggg atcgtccttt ttgtgtggcg ggagaaaaga ataaaatatc cggttcttga
     2881 tgttggcctc tttatgaaaa acaggtcctt taccctttcc aatatagcag cgctcatcaa
     2941 ttacgccgca acctttgcag ttgcatttct actgagtttc tatcttcagc acattaaggg
     3001 atttgcacct cagaaggccg gactggtact tgtttgtcag ccggttgttc aggcagtcct
     3061 ttcgccgctg gcaggacgcc tctcagatag aattgagcca agggttctgg cttctatagg
     3121 tatggccatt gctgccctct gtctcttcat tctcacatcg ttcacgccgg gtacaagcct
     3181 tgtttttatt atctcaactc ttggcttatt tggtgtaggt tttgccctgt tttcttcacc
     3241 taatgtgaat gcaatcatga gttcggtgga ggacaggctt catggcgttg cctcctccat
     3301 ggtagccacg gtgcgcaccc ttggccagat gttcagcatg ggtattgcaa tgctcacctt
     3361 tgcattattc atgggacagg tggaaatctc gccggcgttg tatccacttc tcctgaaggc
     3421 catcaagact gcgtgcacaa tttttgggtt tctttgtgtg gtaggaattt ttgcctctat
     3481 ggcaaggggc aacctcagat aaatcaaaca catggcaatt gactttgtat aacacaacag
     3541 gtaggtttta aaagatggaa ggtctgttcg agtcatctgt aaagggcccg gttgaaaaga
     3601 cacaccctgc attctggttt gtcttcaagg aaaaaaggat gctctgtgtg aagcaggaag
     3661 cagaaaacaa cccccttttt gttgtagaca taagggatat cggcattcat cctgaccggg
     3721 aactctatct gggactgtta gatggatttc attgctatgt tgcggaagta agtgagccaa
     3781 cccgagcccc ggaaggcatg gcatttcacg ggttatggtc tattcacggc agcatggatg
     3841 agaatctatt taaggtagct tttagagcaa tccacacatt ggaatgggac aggacagatc
     3901 agtattgtag tttttgtgga tcacgcaaca gacagaaagt ccaagaacgg gcaaaggaat
     3961 gcccacaatg cggtcgcgta agttttcccc gtatctcccc cgcaattatt gtgctcgttg
     4021 agcatgatgg caaggcacta ctcgcccgct cacctcgatt caaagaaggt cttttcagcg
     4081 ttctggctgg ttttgttgaa cccggggaga ctcttgaaga tgccgtgagc cgggaagtaa
     4141 aggaggaggc gggcatcgat gtaaaaaaca ttcgatattt cggtagccag ccctggcctt
     4201 tccctgattc attaatgatc ggtttcactg ctgaatatgc tggtggcgac atcaagatcg
     4261 ataacgatga gattctggat gcccggtggt tcagcgcaga agacatgcct gagatccccg
     4321 gcagaattag tatctcccgc gctctcatcg actggtttct ggacagacaa aaagggaaaa
     4381 aaacatgaaa aaagtatcaa atggtgtaga attgcaggta gagttgccat aaaaggggta
     4441 caagatgtcc aaaatcatct cagaaaaaag tacgaaaaca gcaatactga atccgtatca
     4501 tgaattattt ataaaagtac gggaacagta atcccttgat gccaaagcag ttaagaaaga
     4561 ggcaggcgaa caacaggtcg tcaaggcggc cctaaacaac tccggggaac agattgtaaa
     4621 acagcacact gaccttaagc tggaaactgt caacagtaag gacagcttgg aaaagaagct
     4681 tataaaagag cacagaaaac agactgaacc ccagcagact atagaggttc cggcgaacct
     4741 gctcaaagag ctctaccaga taactgcttt ctgctctggc gattgcccag cacgagaagg
     4801 agaacgccct agacctccag atggaacaaa aaaaggggat tttgaggcag aaaaggcatt
     4861 aaaaagtacc cagtggaaga aagaacaaga ggagcatgaa gcagctaaga aagaacggaa
     4921 tgcccaagtg aaaaaagaac ggacccggga ggcggtgagg ggagaagtga agaccccagc
     4981 gatataaaac acaatctttg tgataattgc ttttagcggt tgtttgtttc agccgaaaac
     5041 ccattgctga gagtttcctt ttgagactga ggtacaatcg tacctgatat ggccgcatct
     5101 tttcgccttt ctttcatgtt cgaagaactt ttcctgagct tttcaggcca acacagttac
     5161 caccccaatt atttattgca agttgcctct tgacttaaac gcatccatgc cgtcataatc
     5221 tatcatgcga ttcatagccg atcttcatat tcactccaag tactcccgcg caacaagcag
     5281 ggacatgtct ccggaggtca tttggaagtg ggcgcagata aaagggatcg gagttgtagg
     5341 tacaggagac tttacccacc ccgggtggct gaaagagctt tccgaaaaac tggagcctgc
     5401 aggcgaaggg cttttcaagc ttaagaagga acatgagctg aacgatgtgc ccgactcctg
     5461 cagggcagag gtttcattta ttctttcctc tgagatcagc tctatctacc ggaaaaacgg
     5521 cagaacgagg aaggtccatt ctctcatcct tgtcccaggc ttttcagatg cagcccgaat
     5581 caatctctct ctttcgagaa taggaaattt aaacgctgat ggaagaccca ttcttggcct
     5641 tgatgcactg gatcttctta aaattgtaaa ggaggcttct ccatcagcca tctttatccc
     5701 ggctcacgca tggacaccac acttctctgt atttggcgca gcgtcaggat ttaattctct
     5761 cgaagagtgc tttgaaaact atgctcccca tatcaaagcc attgagacag ggctctcatc
     5821 agacccgcct atgaactggc gtgtctctgc acttgatact attaacctca tttcgaattc
     5881 agatgcccat tcacctgcaa aaatgggtcg tgaagcgaac atttttgata cagatatgtc
     5941 atacaattct attgcgagcg caattgaaac aaggcagggc ttcctgggga ccatagagtt
     6001 ttttcctgag gaagggaaat accattatga cggtcacaga gcctgcggtg tcagcctctc
     6061 accggaagag acaatacaaa acaattatct gtgtccaaaa tgcggacggc gagtgacggt
     6121 tggtgttctt cacagggttg agaaattggc agacagacca tcaggatata aaccccataa
     6181 tgcaaccccc tattttcccc tcattcccct cacagaacta attgcttgca cgctgggact
     6241 tggggtgaca agcaaagctg tagaaatgga ataccatcgg ctcatcgtga cctttggtaa
     6301 tgaattttct attctcatgg acgctcccat caccagaaat acgcagagga actggggact
     6361 tattggagag gctatacgta gaatgagatc cggaaatatc aacgtatcct ccggctatga
     6421 tggacagtac ggtaagataa atatcttcaa tgtaggcgag agagaagaga taaaacgtca
     6481 agcgactctt ttctgaccta aacacaatga aggcaattgt ttttgatggg caattgaggt
     6541 ttagcagaaa tcatcctgta cctgagccgg gcgatggcga ggctctcgtt aaagtttgtc
     6601 tggccggcat atgcaatact gatcttgaga ttattaaggg ttacatgagg tttcagggcg
     6661 ttataggcca cgagtttgtt ggtgttgtag aaaagacatg ggaaaaccag cgcctattta
     6721 ttggtaagcg agtggttggt gatatcaatt gtggatgcgg agcatgccta tactgcaagg
     6781 ctgggctgca gcgacactgc ccttcaagga ccactttggg tatagccggt aaaaatggtg
     6841 ccatggctga gtatatcacc cttcctgtgc ataacctgtt ccttgtgccg gaaaacgtaa
     6901 aaaacgaaga agccgttttt gttgaacccc tcgctgcggc atatgaaatc cttgaacaga
     6961 tacatcttag accaacagat agagtgcttg tattgggaga cggaaagctc ggcttgctcg
     7021 ttgctttagt tctggatctt tgtgggcccg aggtggctct ggccggtaaa cacgaggata
     7081 aacttgctat agcaagaggt cagggtgtat gcacagttgc attggaatct cttacagggg
     7141 ataaagcatt cgatatcgtt gtagatgcaa caggctcacc caaaggatta gaagctgcac
     7201 tgcggtttgt taaaccacga ggaacaattg ttctaaagac cacggcggca gaagggataa
     7261 ccatgaacct tgcccctgtc gtcgtaaacg aagtaaaaat aattggttcg cgttgtggtc
     7321 cttttggacc tgccctcatg tgtcttgcac aaaagcgcat caaagttggg ccgctcattt
     7381 ctggtgtcta cggtttttcc catgcaggag aggcctttac caaagcgcag gacaagcgct
     7441 ccctcaaggt actcattgat ttttcaaaat aaaacagggt aacaggctgc aggcaaaggg
     7501 ttatgggagg gttaggccta tgtgcctaat gatgactcga ttgcaaaccg gtattggcca
     7561 aacatttatc tgccatccag cactgtcctt acctttagaa ggagctcttg cggcgctatt
     7621 ggttttgacg tataatcaag cttttcttcg cccatacctt tgtctgtcag aatatccgcg
     7681 gtatacccac tcatgaaaaa gacctttact tcaggcttaa ttgtttttat tacctcgtaa
     7741 acctctcttc cattcttttt gggcattaca acgtcaagaa taacgagatc gacggtctgg
     7801 actgattgtt caaattgcct aattgcatcc tcaccatctc gtgcctcaat tactgtgtat
     7861 ccgaattgtt caaggacttc cttagtaagt tgccggactg tcgcattatc ttcaccgatg
     7921 agaattgttt ctgaacctcc ccgaaccggg ataacctctg gtttgatctc ttcctctggc
     7981 ctcttgacca ctggcagata tatatggaag gttgttccct ggccgggagt gcttttcaca
     8041 ttgatgtagc cattgtgttg cttcacaatc ccataaacaa tggagagacc caacccagtg
     8101 cctttcccca cttcctttgt ggtgaaaaaa gggtcaaaaa tcttttcctt agttgtacta
     8161 tccattccta aaccagtatc agacactgaa ataagggcat acttgcctat ctccccataa
     8221 ccatatgatt caataaaggc atgatcaatc ctgtcaagtt taacttctat tttcatgctt
     8281 ccaccattcg gcattgcatc tcgagcgttc gtcgcaaggt taataagtat ctgatcaatc
     8341 tgtgttgggt ctgccaatat agtcggatcg ctctcactga taaaggtccg gagttcaatg
     8401 tcttctgtaa ggagcctggc gaggagtttc tccacgccct tgatcaatgt gttgattttg
     8461 cacggcttta attcgataac ctgtttacgg ccgaaagcaa gaagactttg tgtaagattc
     8521 acagcctttc cggcagaagc gagaatatgc tctgcgtacg ttcttaaagg atcatcctgt
     8581 gccattttgg ccttgaggag gttgctataa cctatgacag cggttagtat gttgttgaaa
     8641 tcatgtgcca ctcctcctgc aagctggcca atagcttcca ttttctgggc ttggcgaagc
     8701 tgagcctcta aatggctttt ttccatctca gtgcgtttgc gttcagtcat atcctcatag
     8761 atgacaacga tcttcttgtc agtgagatca tttcctatcc tggcgctgct taccatgcac
     8821 agaatgtctt tcccatcttt ccttcgacaa tcaaacattt ctctgtgaaa tctttgcttc
     8881 tctaaaacag gatagaaccg tttcccaatc tcttcgtatt cctcatcgct acgataaagg
     8941 atgcgcgtat ttctgcctat taattctttc ggcgtccagc caaaaaccgc ctccacagat
     9001 tcgttggcaa aaatgattgt tcgtgcctga agcccgatca ccgcatgagg gatagcactc
     9061 agaatagacg attccaactc ttccagagat ttcaaggaat cctcatactg tttgcgctcc
     9121 gtaatgtttt ctaccgttcc ttcataatag agcacatccc cgcttttgtt gagaaccgca
     9181 cgggcattaa tggaaatcca tatacttttt ttgtctttcc tgtaaacttc tgcctcaaat
     9241 ccttttacca cgccatcctt gtcgagaaga gcacgatatt ccgaacgccc ctcagggttc
     9301 acataatgct ggtgcgcaat atcagtgaag ttacgtatca tctcgtcggg cgtctggaaa
     9361 cccatcatat gggctaaagc tggattaaca tcaatgaacc gcccctcagg ggtactctga
     9421 aatatcccct ctacagcgtt ctcaaaaatg tttctgtatt tatctactgt ttctttgagt
     9481 gcctgttctg cccgcttgcg ctctgttaca tccctgaggt ttacaatatt tgcagataac
     9541 ccgttgaact gtatcttcgt tccgaggctc tcaacccact tttccctgcc ttttttagta
     9601 atgatcttgt attgccagag aaaaccaccc ttatcattgt ttacgcgatc aagatgctcg
     9661 aatgctgttt gctcatgctc tggatggaga aagtctcgta tgttaaactc tgtaacattc
     9721 acggggacct caatgtctac cagtctcgcg gcagcattat ttgcaaagag tacgttaccg
     9781 ttccagtcca gtatcaaagt tgcatcaagc atgttgtcga ctagtgagcg taaaagctgc
     9841 tcgctttgca gcaatgcgtt ctccattttt ttacgctcgg tgatgtctac aataattccc
     9901 cggagtccta cacgcaaccc gttacggatt atgggcgaag agttaacgat tgcagggaag
     9961 gtgctaccgt cctttcgttg cattgtgtac tcgaaaccag ggctctttcc caaaattgac
    10021 atctttatgt tttccatggc aagctggcgt tgctcatgga caaccatctg caatgatgac
    10081 aatccgtttt taaactcgtc ataagtgtag ccaaattttt ccaaggcaca tttattaacg
    10141 tatgtaagtt tgccttcatc atcaatttca aaaacacttt gcggtaacag atcggcaagt
    10201 tccttataac gttcgtcttc tgaataatca gcagcagtca tatattatca atattctcta
    10261 caattaaagt tataaaatct actgtttttt aagcaaacca tgtgccagaa catatagttg
    10321 tgtatttcca gtttttgatt gtacaagata ttttttgaca aacaaaaagc gaaaggatac
    10381 attctcttgg tgtggcttat acttgtactg tttttgatcc cattcagtgt tcatggctat
    10441 gaccgtatca tatctctttc accacagata accgagtcac tttacctcat agaagctgca
    10501 aaagaacttg ttggtgtgac agagttctgt aagaagcctg cagaagcaac aaaaaaacag
    10561 aaggtgggaa ctccactcag gccagatgtg gagaagatcg tatctatgcg cccggatatt
    10621 gtctttggaa cacgagaagg gaactcaccc cttgtcatgg cacggcttga aaggttaaat
    10681 attaaaatac attattttaa tcggccaaaa acatttgcag atttgatgga caattttttg
    10741 acgctttcaa aatgtgtggg taaggaagaa aaaggaaaac aaatcgtaaa taatgcacgg
    10801 gaagcccttt caaaactaaa aaatgaaaaa gggtatagag tgttgtggca ggtgggggcg
    10861 gatcctttag tagttgcttc gaataatagc tttgccaatg atattatagt attggctggt
    10921 gggtccaata tcattgaaag cgagcttcca tacccgagga tcaatactga ggaggtgatt
    10981 ctgaagaatc caaacgtcat tgttctcatg gacatgggtt ataatgttca attggaaatg
    11041 gagcgatgga aaagatactt taacaaagta aaatttgtga tcctggatgc ctattccgtt
    11101 gggagtccaa caccagtaag ttttgttaat gccgttaaaa cacttgccga actgtttcca
    11161 tgagaagaaa acaaaaaagc gttgtagttt taacaatcct tgtctgtctt ttgcttgtct
    11221 caatattgtt gtccttagca tatggcaagg gaattggtgt gatgtttcac agctttttca
    11281 ataacgatgg ttttggactt attctcagaa aaataaggat accgcgcacg atcattgcat
    11341 ttcttgtggg aggctctctt ggcgtttgcg gtgtaacctt acaatcaata ctgcgcaacc
    11401 cccttgcaga gccatacacg cttggcatag caggaggcgc atctttcggg atagccctgt
    11461 cgacaattat tggttttcaa cgaatatttg gtgcatttgc taaccccctt tttggttttg
    11521 gtggagccat gctgtcggtt gcaatagttt ttgctctctc aaaaaaacgt ttttttgacc
    11581 ccaacagcat ggtgctgttc ggtgttgttg tgagtcttgt tttttcatca tttgttttct
    11641 ttcttttctc ccttctcgac cctgataaga tgcaaacaac ccttatgtgg cttatgggag
    11701 atctttcaag tctcgacata gcatttatcc ccatgtatat ccctgcgttt cttatcccga
    11761 cctcaattct tttctttttt ggcaaggagc ttgatatcct ttcccttgga agggagaagg
    11821 cacaatacct tggtgtaaat cctcccagat tttacaaagg acttttcctt ctgtcgtccc
    11881 tcctcgccgg cctgtgtgtt tcagcttcag ggattattgg ctttgtcggt ctcattgtgc
    11941 cccatatctt aagaaacatc ataggtgccg ggcatactgt cctcctcata gcatcttaca
    12001 ttggcggggc tttctttctc atcctttctg atgtgttttc ccgctatctc ctatatcccg
    12061 tagaacttcc cgttggcgtt gtaaccggaa ttcttggagg atttattctc ctttttctcc
    12121 ttatacggag gaagtgatgg gagatgctct tttctcagtt catggcgtag tttttaatta
    12181 cgatcgtcac acagctattg atgacattac cttcacggta aatcctggcg aattcatggg
    12241 cattattggg ccaaacggcg caggcaaaag cactgttatc cgtcttcttg caggattttt
    12301 gaagccgaat aaagggtttg tagcgttttc cggcaaaaac ctcgataaat atgggaaaag
    12361 agagttggct cagaatattg cgactttacc acaagcaaca gaaacgccct tttcatttac
    12421 cgtagaagag ttcatcctca tggggcgata cccctatgcc caaaaaaact ggctttatga
    12481 aaaaaaagaa gaggagtttg tagaatacat catagatact atggaaatct cacatctgcg
    12541 tggtcgaaca atcgacaccc tctcagaagg tgaaaggcaa aaagtgttcc tatcccagtg
    12601 tcttgctcag gatcccaagg ccctgctcct tgacgaaccg gtgagccacc ttgatatacg
    12661 ccaccagatg cagacttttg atgttctaga aaaactacac agtgaaggtt tgacgattat
    12721 tatggttctt cacgacctca atctggccgc tgaattctgt tcgagactca tgcttatatc
    12781 aaaaggaagg ataaatgcag atggagaccc caagacagtg ctgacttttc aaaatataga
    12841 agaagcatat gacactgtag tgattgtcaa ggagaaccct ctctcaggaa aaccatttgt
    12901 tgtccctgta tccaggaaat atctggggaa ataacgctgt ggagtaaatc ctcacgtgcg
    12961 aaatatgccc ccgaaaacac ggggtaagat ttcttcgcgt ccctattctt tccagtcaaa
    13021 gaattctttg aatttttctt ttatgccgcg cctggatgga ttaacaatac cgttaaattc
    13081 ctgtgcaagc tcctgaagta agtgccgttg tcgctccgta agctcagttg gaatgccaac
    13141 attgagataa acaagctcat cgcctctccc gtatccgttg gactttgtta caccgaggcc
    13201 cttaatgcga aaaatcttcc caggctgtgt gcctggtggg atgttcaaca cggtctcacc
    13261 ctcaacggtg ggaacggtaa tatcaccccc aaggcagaga agagggaaat tgacatcaac
    13321 ttgaacggct atgtcatcac cttcacgttc aaagataggg tgttccttaa cgtgaagcac
    13381 cacataaaga tcaccaggca cggttgctgc aaaaccctga gctccttctc ctctcagctt
    13441 cagacgggcg ccattatcaa ctcccggcgg aacttttatt tttagctttt tctttgtttt
    13501 gattgagccc ctgcctttgc aagtcttaca gggatcttta atgataaagc cttccccatt
    13561 acagtattcg catgtccggt tgattgtgaa aaagccatgg ctttgtcgta cctgccctct
    13621 acccgaacat gccttacaaa cagcaggttt gtggccgggc tcgaccctcg acccgttaca
    13681 ggattggcaa cgctcttcct tcggtacttc tatctctttc tcaactccaa agacagcctc
    13741 ttcaaactct atttggaggt tgtatcggag atcctcgcct tttttttcac gttgtcgctg
    13801 gcccccaaaa aaatcactga agagatcatt aaaaatatca tcgaaattgc ctctgaaccc
    13861 gaagtcgaag atgctccctg aatcgtcagc acttccaaag cgttcatacc ggaagcgttt
    13921 ttcctggtca cttaaaacct cataagcctc gctgatttct ttgaaccttt cctctgcctc
    13981 tttattgtgg ggatttcggt cgggatgata ttttaatgcg agctttctat atgccttctt
    14041 tatctcttca tcgcttgctt cacgatgaac gttgagtatc tgataatagt ctttccgcac
    14101 ggttgtacct taacgaatca ctgggcctca ggtttttttg acacagagac tctcgagggc
    14161 cgtagtagtc ttccattgag ggtatagcct ttctgtaatt ctgatatgac aatgttgggc
    14221 tccatattct ctgtttcttc ctgaaaaaag gcctcatgaa aattaggatc gaattgcttt
    14281 cccattgcat caatcctctc aacgcccgac ctttccaaaa ctctgagaaa ctcgttgagc
    14341 gtaattttta cgccctcaac tattccctga tcatcttggt acttttcagc atggtctatg
    14401 gccctttcaa ggttatcgat aacaggcagc aattccttaa tgagaacttc attgccgaac
    14461 ttgaggaggt cctgcttttc cctggctttt atttttttaa aattttcaaa atccgcctgc
    14521 agatacagat atttgctatt cagctcttta aaggattctt ctttttcttc aagaatcttc
    14581 tgaagctctt cagctacatc gtctttcttc tttttcttct tatgatcttc gtggggctct
    14641 cccttttctt ttgtcttttt ctctgaattt ttttcttctt tttcttccat ccttaaccct
    14701 ctcaaacggt tgtaagaata ttcgtaactg tgttggcggc atatgttacc aggggaataa
    14761 tctttgaata attcattctt atgggtccta ttacgccaag gactccaaaa ctttgttcac
    14821 caactctgta tgtagaggta atgatactca tatcgctcat ctctttaatg tcgctttcgc
    14881 cgcctatgat cacatggata ccttcttcct ttaaacaact atcaatcagc tccagaagct
    14941 tttccttttt ttcaaaagcc tgaaaaagcc ctttcagacg ctcaacatca gcgaacccgg
    15001 gcaccccaat aatctttgag gcgccttcca gatagatttc cctgacatct tcctcgtcga
    15061 taatcgtttc cagtgtttgt tttatctttg ccaggagcct gctgtagttt tccctgtcct
    15121 tctttatatc ttccagaatt ccttgcttga gggcgtaaaa tggcatgcca ccgaattttt
    15181 cgttcatgta ggccttcatg ctatgcaaca tggccacatc aaattcctcg tctgtgttaa
    15241 ccagtcttgt gtggaccata ccggaagagg tcacaaatac agccaggaca gtcctgcttg
    15301 aaagctttac aaactcaact tctttgaaaa gcatcgtgtc tacttttggc tcaacaacaa
    15361 tacttgtata ttttgatagg gaagccagaa ttttcgatgc gtcttccatg atttcctcaa
    15421 tataagaata atgaggttta atcatagttt ctaaggctat aagctctttt tttccgggaa
    15481 gttttagtaa agacaggcta ttcacataat aacgatatgc tttgtatgta ggtatccgtc
    15541 cggctacaac atggggtttg tataggaatc cttgttcttc aaggtctgcc ataacatttc
    15601 gtatagtagc tgcactaagc ctgtccttta tggtttttgc tattgttccc gagctgatag
    15661 gctcagcaga attgatatac ctttccatta tcaattcaag aaccattttc tctctttcat
    15721 ttaagacgtc cactgcatgc cccagattac accattaact taaggatacc aaattttttt
    15781 gtcaagcaac acagccattg ctgcgtgttg ccaatacagg aatattttcg tattattata
    15841 cacttattct gctttagctt tcaatcatct ttcgtaaccc
//
LOCUS       DAOOMC010000066        15702 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdus sp. isolate AMR_MDS_2685
            NODE_3961_length_15702_cov_6.409024, whole genome shotgun sequence.
ACCESSION   DAOOMC010000066 DAOOMC010000000
VERSION     DAOOMC010000066.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864760
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdus sp. (wastewater metagenome)
  ORGANISM  Syntrophorhabdus sp.
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae; Syntrophorhabdus.
REFERENCE   1  (bases 1 to 15702)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15702)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,729
            Genes (coding)                    :: 2,708
            CDSs (with protein)               :: 2,708
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 7 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15702
                     /organism="Syntrophorhabdus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3961_length_15702_cov_6.409024"
                     /isolate="AMR_MDS_2685"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2699756"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(295..990)
                     /locus_tag="PK125_08030"
     CDS             complement(295..990)
                     /locus_tag="PK125_08030"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HPB38094.1"
                     /translation="MADGTRTYKIIRSKNRKKTMTLQITSEGNIVIRTPLRTPDSEIE
                     HFFQSRKIWIAKKIKAREVDREIQDSPRNFTEGEKFYYLGDSYPLEITEPKGVRPPLI
                     LTHGKFLLTREKAPHAKELFVKWYRNRAREIIGDRVRFWSTRFNLVPTGITITSAWQR
                     YGSCSAQNSLSFSWRLIMAPYEVIDYIIIHELAHIKEKNHTVRFWQYLESLMPEYKKQ
                     RHWLRENSHMFRL"
     gene            complement(990..2369)
                     /locus_tag="PK125_08035"
     CDS             complement(990..2369)
                     /locus_tag="PK125_08035"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HPB38095.1"
                     /translation="MKIRFLGAARTVTGSCFHLIDNGLQVLVDCGMYQGKQGDENNRK
                     PFAFDPTKIDYLLLTHAHIDHSGLIPKLVKDGFKGKIITTTATSDLLEIMLYDSAHIQ
                     EKDAEWLTKKSLRSGKDEVFKPLYTAEDVAACVPFLERKQYRIVEDLGGIRYRFVDAG
                     HILGSASLEIWFQSAAGEKKIVFSGDVGKNENPIIQNPEHIDEADYVVVESTYGNRSH
                     RGMEESIDEMVDAIKTTFKKGGNVLFPAFAVGRTQDIIYILNKLVKEKRLDGLDVYID
                     SPLAEKATSIYMAHPEFFDAEASSHFNITSSAGMKIHFTRTVEESQKLNKIKSGAIII
                     AGSGMCDGGRIRHHFKHNLWRSECSIVFTGFQVRGSLGRLIIDGMKSVRVLGEEIAVR
                     AKVYTIGGFSAHADQKELLEWLGSFKNRPEIFIVHGEENVALQFEKIVNERLNLKTRV
                     PMRGDEIAL"
     gene            2897..3892
                     /locus_tag="PK125_08040"
     CDS             2897..3892
                     /locus_tag="PK125_08040"
                     /inference="COORDINATES: protein motif:HMM:NF033779.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tim44 domain-containing protein"
                     /protein_id="HPB38096.1"
                     /translation="MIQTANLPWKMVVLFFALFFTFFLGMESLAHARAGGGRSMGSRG
                     FTSGSGSRTYQGTTTRPPQPTQQPGMTQPPPMQPSSPGMGRSFLSGIGGGLLGGMIGS
                     MLFGGRASAAGQGRGGFGLGDLIIIFIIIAVVYLVIKRFKVQKSMEMSSTGTAYAPSS
                     YPYSEPATDTYYEPSSRDGAIAQGLSHISEMDPSFNEARFKETAEDMFFKIQGAWTRR
                     DLTSIRNLLTPQMFNTFQDDVNRYIADKQVNRLENIAVRQVNIVDAVQDQGEEYITVK
                     FLASLLDYVVDETNGHTISGNTSDPVKFLEYWTFTRKVGQRNWALAGITQEGDYP"
     gene            3929..4477
                     /gene="idi"
                     /locus_tag="PK125_08045"
     CDS             3929..4477
                     /gene="idi"
                     /locus_tag="PK125_08045"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: protein motif:HMM:NF002995.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004452 - isopentenyl-diphosphate
                     delta-isomerase activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HPB38097.1"
                     /translation="MTDEIILVDHLDNEIGFTEKDPCHRIPVRLHRAFSIFIVNDRGH
                     MLIQKRSSSKKTWPGFWSNACCSHPRKGESLLQATSRRLDEELGFTCPLQHITSFRYE
                     AAYDKELGEHEIDHVFVGHFGGLIQPNKDEIEDLKFIPLEKLTYDIDKNPEKYTPWFK
                     KALPGVLEFLKNHSQKGAGSGS"
     gene            4508..5089
                     /locus_tag="PK125_08050"
     CDS             4508..5089
                     /locus_tag="PK125_08050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB38098.1"
                     /translation="MKLKIYITALVIVVFLALGFFYVRSTPQYSLYMLKRAIEERNPN
                     EALKYINIDSIVESIARSLLNKNEKDPGQDTNKRTALKGVLIGALPEIKESIRSSLHK
                     AILSRGKSIAKSNPDNATGITDQRAHGFSIGEIAIGNFDVRRIEKISLWDLTVKNNGG
                     TAIVSVKNNPGIKAKMARIDTGHWQITEVLLLP"
     gene            5220..6734
                     /locus_tag="PK125_08055"
     CDS             5220..6734
                     /locus_tag="PK125_08055"
                     /inference="COORDINATES: protein motif:HMM:NF012784.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HPB38099.1"
                     /translation="MIEEKLENQLTDSAPEAEINTQEESFAELFEKDSKIPGRLEPGQ
                     KVTSTIVSISGDFVYVSLGGKSEGVIDIAELRDEEGQTAVNVGDSIDSYFVAVQGGLK
                     RLTTLRHGLSTLSFKEIRDAYEAGLPVSGKVVKELKGGFEVNVGKIRCFCPFSQIDMK
                     GFRDTVTYIGETFSFRILEFQENDRNVILSRRALLEEEQEAKVDALKKQLAVGMDIPA
                     KVRSIQSFGVFVDLGGIDGLIPISEIGWGRVDNIQDILQPGSEVTVRVMALDWDRERL
                     TLSLKALQPDPFLDATAKYPVDSLVRGTIVRLAPFGVFVNLEPGIDGLVHISKLGTGR
                     RIKHPKEVVAVGQVVEAYVQEVDVGNRRISLSMEQKVEPGAIMLPEAGELLSGTVEKV
                     LTSGVLVKLPGGAIGFIPNSEMGTPRGTNHGKMFPAGTPLQVMVVEVNQDRGRITLSR
                     SGVEERLEKEELTSYMKQAQQDEKESRSLGSFGELLKAAMDKKASKKGELTSQK"
     gene            complement(6740..7078)
                     /locus_tag="PK125_08060"
     CDS             complement(6740..7078)
                     /locus_tag="PK125_08060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB38100.1"
                     /translation="MARNCPHCDSEMFEEYDHINRVRVLVCSRPTCLFRSYPDYPRRK
                     SNEEVCYKCQSIFKVRQNDTGILCPECKAVIEQNKKGQGSAKEQKPFKAAHGETFLRE
                     SPKAKLRRAV"
     gene            complement(7365..8045)
                     /locus_tag="PK125_08065"
     CDS             complement(7365..8045)
                     /locus_tag="PK125_08065"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPB38101.1"
                     /translation="MSEPILRVSGLKKTFSKNGVEINVIKGISVDINEGDFITIMGPS
                     GAGKSTFLHILGTLDKPSEGDIFFRDKNIKEFSEDEESRFRNEKVGFVFQFYHLLQDF
                     NVIENIIMPLLIRRLSRNDAVAKAESFLEIMGLQHRREHKPGELSGGEQQRVAIARAL
                     VNEPEIILADEPTGNLDRKTGKEVLDYLLSINDRLSSTLVLVTHDPEIGSIGERKMKM
                     VDGELFSV"
     gene            complement(8038..9243)
                     /locus_tag="PK125_08070"
     CDS             complement(8038..9243)
                     /locus_tag="PK125_08070"
                     /inference="COORDINATES: protein motif:HMM:TIGR02212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit"
                     /protein_id="HPB38102.1"
                     /translation="MNYETTVGLRYLRSKRKEAFISFTTWISIAGIAIGVMALILVIA
                     VMTGFQNEIRERILGINPHVLILGLAGDIKDPEKVVSAVKDIKGVTHAFPFISFQAMV
                     QTGKQLSGALVKGLNPGDVQFMSRLLKQGSIGALNQRGNILIGKELSKHLGVLYGDFL
                     TIMVPFGGVSPMGAVPETVRVKVGGVFETGVYDIDNSLIIMSIQDIEAIMGQGVTGIE
                     IKIDDVYQADSIRKEIMTRLGSATNFFARTWIEMNRNLFSALKLEKIAMFIILALIIF
                     VASFNIISSLIMTVMEKKKDIAILKAIGAKKASLMKIFMVEGITIGIFGALIGSFSGY
                     ILCEIQRRFSIVKLDSDIYYINTLPIKISILDTFLVASITTIICILATLYPSYKASKI
                     DPVETLRYE"
     gene            complement(9319..10782)
                     /gene="lysS"
                     /locus_tag="PK125_08075"
     CDS             complement(9319..10782)
                     /gene="lysS"
                     /locus_tag="PK125_08075"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HPB38103.1"
                     /translation="MEPINELVAVRKDKEKYFRSIGIDTYPQGRGSYVDTASIAQRFG
                     AMSHDDLEKEGATLSVAGRIVAFRDFGKSAFLHIQDKKGRIQVYVRKDMLKGLPYEAF
                     KKFDIGDIISAEGKIFKTKTEELTILAERISLLTKSLRPLPEKWHGLKDIEERYRKRY
                     LDLIVNEKSRKVFEERAIIIEYIRNFFASRDFMEVETPMMQVLPGGAVAKPFITHHNA
                     LGMDLYLRIAPELYLKRLIVGGLERVFEINRNFRNEGISVRHNPEFTMLEFYQAYATY
                     EDLMTFTEDLVSSLAVKLSGRTKIVFNGQEIDFTAPWKRMTMEEAMRDLGGFDLQKSH
                     DLGTLTSYAKSLEIEGVEKDAKGKIITKIFETLCEEKLIQPTFITHYPVEVSPLAKRS
                     KDQPDVTERFELYIAGMEIANGFNELNDPEDQRQRFMEQVKDREEGAVLDEDYLAALE
                     YGLPPTAGEGIGIDRLVMLLTDSPSIRDVILFPLLRP"
     gene            complement(11102..12370)
                     /gene="rimO"
                     /locus_tag="PK125_08080"
     CDS             complement(11102..12370)
                     /gene="rimO"
                     /locus_tag="PK125_08080"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HPB38104.1"
                     /translation="MKFKIISLGCPKNLVESEYISFRLEKDGHILSDDCNTVIINTCA
                     FIRDAAQESIETILMAAKERNKKERKIVVTGCLVERYREELVNLLPEVDLFVGRGMYH
                     EIDKVITEAGYFFQDTPFYESYPRKVLTGKPSAYLKIQEGCNNRCAYCTIPSIRGDLR
                     SRTIADIGAEFTYLLENGYREISIVGQDITSYGMDGKFDLKTLLRHMLKVEGDFFLRL
                     LYMHPKGVDDELIDLIGESEKIIPYLDIPIQHSEDRILSLMGRGYTRKQLDCLFQKIK
                     DRIPNATLRTTVLVGFPGETENEFAGLCDFVKKCEFDMLGAFTYSREEGTRAFRFKGH
                     LRKNAKQKRYNTIMEMQREISGRRLGRLVGTKNFVIVGGYEKPYMTGRIITQAPDIDG
                     MAFIKGKCAPGEIRQGKIVKTLDYDVIVEV"
     gene            complement(12377..13111)
                     /locus_tag="PK125_08085"
     CDS             complement(12377..13111)
                     /locus_tag="PK125_08085"
                     /inference="COORDINATES: protein motif:HMM:TIGR02757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02757 family protein"
                     /protein_id="HPB38105.1"
                     /translation="MVRNDPVEFLHRFKSSKDKELAGFLSSQFAYGKVGVLRQFLEKL
                     FSLMGESPWKFVMEGDFSSLKGLYYRFQKADDIVYLFIILRRILEYFDSMGAMLQNFY
                     TGDTRKMIWQIRDELIGSDERLTFFFPKPSESNPMKRWSLYLRWMVRKDEIDVGLWDF
                     IPKNRLVIPLDTHIFKIGKCFGWTRLKSPSWKAACEITDVLKSFSPDDPLRYDFFLCQ
                     SVGINGKCTGVKTTDCIEKCLLLQNS"
     gene            complement(13205..13756)
                     /locus_tag="PK125_08090"
     CDS             complement(13205..13756)
                     /locus_tag="PK125_08090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB38106.1"
                     /translation="MAEIKSAIELAMERTKNLVVDPKEREAMAIKEIEDKVKAVLRRF
                     AESMIDHEGAAKELGKIQGDMSSKRTIIIKNLIDEFDVHKDNERLFAIFHAAGIDLPQ
                     SIRSDLETINRKFREEIESRRTAIQKRIRDALAELNITGSAIEPNLDAWDEWDEEVRR
                     ASEAFGATMKELKNKARAATESR"
     gene            13922..15112
                     /locus_tag="PK125_08095"
     CDS             13922..15112
                     /locus_tag="PK125_08095"
                     /inference="COORDINATES: protein motif:HMM:NF015684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HPB38107.1"
                     /translation="MVNFLVQITKAYKRFYLYLLKELTYILLLSLAILTFILVMSRLG
                     KLTDLVINKGVGLMDIVLLIVYSSPPYLTFTLPMAFLLSTIVTLGRLSTENEILALKA
                     SGVNLRCLFVPIASVGIIISAVAIFNTNFLLPTSANLFRETLLNVLKKGIAVDDKEGI
                     FNDSIPGVVIYIDRVDTQTNRLTGILVSDDRDKDEKQTISASTGFIHLDPATLDLSFI
                     LYNGSLHKWEKKSDTYRTVSFKDYNFMINLANMLPPSAGIRKRPFEMDRSELKNAMIR
                     ATKDRKYDLQIEIYKKITIPLSALAFVLLTVPLGIRRKVEGRFSGVVYSLALFVFYYM
                     LMALTENLGRALYVPPIFIAAIPNTTIVVLGLYLLRNLNVEEPAKTSHLIRSFWMRYH
                     EKAK"
     gene            15096..>15702
                     /locus_tag="PK125_08100"
     CDS             15096..>15702
                     /locus_tag="PK125_08100"
                     /inference="COORDINATES: protein motif:HMM:NF015684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HPB38108.1"
                     /translation="MKRLNRYLILNVLKLLLLTQFAGLVMFVAIEFLEHMDIFTDSLN
                     SFLLSLVYILLRLPHYFNLLLPLASLISMLILLILMVRNNEIIAARTSGISTISLMKP
                     FIACACGLIIFTFIIAEWIMPYAANASEYLYRVKIKKEDSYIHFKNDRIWFKRNNVLY
                     NIDYYDMKKDVIRGLTVIELADDFTIQKRIDAKEGIWKDSQW"
BASE COUNT         4309 a         3591 c         3260 g         4542 t
ORIGIN      
        1 taagtttttc ttgagggtaa atacaggttt tgggttaatg aagccacagg caaaacattt
       61 gtgcctaaat gaccttggaa caacaacctg tgatcaggga gtttctgacg gtatatttat
      121 gtgtcctaaa gtggctaaga cattcggcca gtagctgctc aaacaggtgg agtaaatgag
      181 acaacaggta ccaccgggaa ggtggaagca gtatgcatca cggaaataag cacgtgacta
      241 atatttcaaa tcagcctaaa aactatcagc ttacagcttc aggctatgag tttctcacag
      301 acgaaacatg tggctgtttt ctctcagcca gtgtctctgc tttttatatt ccggcatcaa
      361 gctttccaga tactgccaga accgtactgt atggttcttc tcctttatat gggcaagctc
      421 gtgaataatg atataatcta taacctcgta aggggccata atgagccgcc acgaaaagga
      481 aaggctgttc tgtgccgaac atgaaccata tcgttgccag gcgctggtta tggtgattcc
      541 cgtcggcaca aggttaaagc gagtgctcca gaaccggacc cgatctccaa taatctctct
      601 cgccctattc ctgtaccact taacgaagag ttcttttgca tgtggtgcct tttccctggt
      661 aagcaaaaat ttaccatggg taagtattaa gggcggccgc acgcctttcg gttcggttat
      721 ctcaagagga taggaatctc caagataata aaatttctcg ccctctgtga aattacgtgg
      781 gctgtcctgt atttctctat ccacctctct tgctttaatt ttttttgcta tccagatctt
      841 gcgggattga aagaaatgtt ctatttcgct atccggagtt cgaagagggg ttcttatgac
      901 aatgttgcct tcgctggtaa tctgaagagt catagtcttt ttccgattct tacttcggat
      961 tatcttatag gtcctggtgc cgtctgccat tataaggcga tttcatctcc tctcattggg
     1021 actctggttt taaggttaag cctttcattg actatcttct caaactgaag ggcaacgttt
     1081 tcttcaccat gaacaataaa tatctccggt cgatttttaa agcttcccaa ccactcaagc
     1141 aactcctttt ggtctgcatg ggcagagaaa ccaccaatag tataaacttt cgcacgaacc
     1201 gcaatctcct caccaaggac acgtacgctt ttcatcccgt caataataag ccttccaagg
     1261 gaacctctca cttgaaaccc tgtaaagact atggagcact ccgacctcca caggttgtgt
     1321 ttaaaatggt gtcttatacg tccaccatca cacatcccac taccggcaat gataattgct
     1381 ccggatttta tcttattgag tttctgcgat tcttccacag ttctggtaaa gtgtatcttc
     1441 atgcccgctg aacttgtgat attgaagtgg ctggaagcct cggcatcaaa gaattcaggg
     1501 tgtgccatgt agatacttgt tgctttttca gcaagggggc tgtcaatata cacgtcgagg
     1561 ccatcgagtc gtttttcctt aacaagtttg ttgagaatat agataatatc ctgggtgcgc
     1621 cccacagcaa aggcaggaaa caatacgttt ccaccctttt tgaatgtggt ttttatggca
     1681 tccaccattt catcgatact ttcttccatt cctctatgag aacgattacc atatgtggat
     1741 tccaccacaa cgtaatctgc ttcgtcaatg tgctctgggt tttgaatgat gggattctca
     1801 tttttaccca catcaccgga aaaaacgatc ttcttttctc ctgcggcaga ttgaaaccag
     1861 atctcgagac tcgcggagcc aagtatgtgt cctgcatcaa caaacctgta cctgattcca
     1921 cccaggtcct caactatccg gtattgtttt ctctcaagaa aagggacaca ggctgcaacg
     1981 tcttctgcag tgtagagcgg tttaaaaacc tcgtcttttc cagatctgag agattttttg
     2041 gtaagccatt cagcatcctt ctcctgaata tgtgccgaat catagagcat tatttccaag
     2101 agatcagacg ttgctgtggt ggtaattatt ttacccttga atccatcttt cacaagtttc
     2161 ggaatcagcc ccgagtggtc tatatgggca tgggttagta aaaggtaatc gatctttgtc
     2221 gggtcaaaag caaaaggttt cctgttgttc tcatcacctt gctttccctg gtacatcccg
     2281 caatccacca gcacttgcag cccattatca ataagatgaa agcatgatcc tgtaaccgtc
     2341 cttgcagccc ccaaaaagcg aattttcacg tgagcacctc atgttgagcg tttttctgat
     2401 tatactcgta tgatacccga tttacaagta gcctttatag atcgggtata agttacaaac
     2461 ctattttccc tttggctgtc ttaccccctg tttgcttata tgggccatgg ctttccctgg
     2521 aacagttatt tttgttgcca tcactggaat tatggtggat ggaatctcct atcttctgca
     2581 gaatcatgtt tgtctctatg acatcccaat gaagggttct atatcaccac atacatgaac
     2641 cactctaaat cgtggacaat cccacccatg acgacaaagt aaaaaaaggt atccacaaat
     2701 tgcaaataca accctcctat taattctgct gaccaaatct tcacgatctt aggcaccata
     2761 agacaacttt agacaatacc aggcaattat tgaatttttg gcagaaatcc agcgcaaccc
     2821 atgatatgca agaccttttt gacaggattt gatagagtat ggtatcctac tgactatcaa
     2881 aatttgagag ggcacaatga ttcagacagc taatctccca tggaaaatgg tggtcctttt
     2941 cttcgccctt ttcttcacgt tctttttagg aatggaatct cttgcccacg caagagcagg
     3001 tggcgggaga agcatgggaa gtcgcggttt tacgtctggt tccggttcga gaacctatca
     3061 gggaacaaca acgcgacctc ctcagccaac ccaacagccg ggaatgacac aaccaccccc
     3121 catgcaacca tcatctcctg gcatgggcag gagttttctc tcaggaatag gtggtggact
     3181 gcttggcggg atgatcggga gcatgctctt tggaggcagg gcaagcgcag caggccaggg
     3241 cagaggaggc tttgggcttg gtgacctcat cattattttc attattattg ctgttgttta
     3301 cttggtcatt aagcgattca aggtacaaaa atcgatggag atgagttcaa caggcacagc
     3361 ctatgcccct tcttcttatc cttatagtga gccagcaacg gacacctact atgaaccctc
     3421 atcacgtgac ggggcaattg ctcaggggtt gagccacatt agcgaaatgg acccatcttt
     3481 taacgaagcc aggttcaaag agactgcaga agatatgttt ttcaaaatcc agggtgcgtg
     3541 gacaagaaga gatttgacca gcataaggaa cctgctaacg ccccagatgt tcaatacatt
     3601 tcaggacgat gtcaaccggt acattgcaga taagcaggtc aaccgcttgg aaaacatagc
     3661 cgtacgacag gttaatattg tagatgcagt gcaagatcag ggagaagaat atattactgt
     3721 aaagtttctt gcgagccttc tggactatgt tgttgatgag acaaatggcc atactatttc
     3781 gggaaacaca tcagaccctg ttaaatttct tgaatactgg acttttacac gaaaggtggg
     3841 ccaaagaaac tgggctcttg caggcattac ccaggaagga gattacccgt aagggaacca
     3901 acaatgcaac aaggtacggt ggtttaatat gacagatgaa atcatcctcg ttgatcacct
     3961 tgataatgaa attgggttca ctgaaaaaga tccgtgccac cgcatacctg taagactcca
     4021 tcgcgcattc tcaatattta ttgtaaacga cagaggacac atgcttattc agaaacggtc
     4081 ttcctcaaaa aagacatggc cgggattctg gtcaaacgca tgttgttccc acccaaggaa
     4141 aggtgaaagt ctgctgcagg caacttcaag gaggttagat gaagaactgg gtttcacatg
     4201 ccctttgcag catatcacgt cctttcgtta tgaggcagcc tatgataaag aacttggaga
     4261 gcatgaaata gatcatgttt ttgtgggtca ctttgggggt ctgatacaac cgaacaagga
     4321 tgaaattgaa gacttgaaat ttattcctct tgaaaagctc acatatgaca ttgataaaaa
     4381 tcccgaaaaa tacaccccgt ggtttaaaaa agcccttccc ggggtattag aatttctgaa
     4441 aaatcattct cagaaaggtg ccggatctgg gtcttgaacc tgccccaagc tctgcgaagg
     4501 gaatccagtg aaactgaaga tctatataac agccttggtt atcgttgtct ttcttgcttt
     4561 aggatttttc tacgtcagaa gtactcctca gtattcactc tatatgctga agagggctat
     4621 tgaagagcgc aatcccaatg aggctctgaa atacataaat attgacagta ttgtagaaag
     4681 cattgcaagg agcctgctta ataaaaacga gaaagaccct ggacaggata caaacaaaag
     4741 aacggcttta aaaggtgtgt taattggcgc tttgccggaa atcaaagaat cgattcggtc
     4801 ttcgcttcac aaagcaattt tatcacgagg aaaaagtatt gcaaaaagta acccagacaa
     4861 tgcaaccgga atcacagacc agcgtgctca tggtttttcc ataggagaga tcgcaattgg
     4921 taatttcgat gtaaggagaa tagaaaaaat ctctctgtgg gatcttactg taaaaaacaa
     4981 cggagggaca gctattgttt cagtaaaaaa taaccctggc ataaaggcaa aaatggcaag
     5041 aattgacacc ggacactggc agatcacaga ggtgctcctc ttgccataat caaatacaat
     5101 atggacatgg ctgacaagaa aaggtaaagt atatagaata agtcttaaat gttaatcgca
     5161 taaaatacaa taaaaacgta gaagatctgt ttttcaggtc tattgagagg tttaaagaaa
     5221 tgatagagga aaaacttgag aaccaactaa cggattctgc ccctgaagca gaaataaata
     5281 cccaggaaga aagttttgct gagctctttg agaaagacag caaaatcccc ggcaggcttg
     5341 agcctggaca gaaggtcacc tcaacaatag taagtatttc aggtgacttt gtgtacgtaa
     5401 gccttggtgg taaaagtgag ggtgttatcg acatcgccga gttaagggat gaagaaggtc
     5461 agactgctgt taatgttgga gacagtattg attcgtattt tgttgcagtc cagggaggtc
     5521 tcaagaggtt gacaacattg aggcatggtt tatcaacact cagttttaaa gaaatccgcg
     5581 atgcctatga agccggattg cctgtaagcg gaaaagtagt taaagaactc aagggtgggt
     5641 tcgaagttaa tgtagggaaa atacggtgtt tctgcccgtt ttcacagatc gacatgaagg
     5701 gctttcgcga tacagttacc tacataggcg agacattttc ctttagaatc ctggaatttc
     5761 aggaaaatga ccgtaatgtg atcctctcac ggcgcgccct cctcgaagaa gaacaggagg
     5821 caaaagttga tgctttaaaa aaacagttag ctgtgggtat ggatattcct gcaaaagtcc
     5881 gctcaattca atcttttggt gtttttgttg atctcggcgg cattgatggc cttattccca
     5941 taagcgaaat aggttggggc agggttgata atattcagga tattcttcag ccgggctctg
     6001 aggtcactgt aagggtgatg gcactagact gggacaggga gcgcctcacc ctgagcctca
     6061 aggccctgca acccgatcct ttcttggatg caacagcgaa atatcccgtc gatagccttg
     6121 ttaggggaac gattgtgcgc cttgctcctt tcggcgtttt tgtcaatctg gagcctggaa
     6181 tagacggact ggttcacatt tcaaaacttg ggacagggag acggattaag cacccaaaag
     6241 aggtcgttgc agtgggtcag gtcgttgagg catatgttca ggaggttgac gtcggcaata
     6301 gaaggatttc cctttccatg gaacagaaag tagaacctgg ggctatcatg cttccagagg
     6361 caggggaact attgagcggg acagtagaaa aggttcttac ttcaggagtg cttgtgaagc
     6421 tgccaggagg tgccataggt tttataccaa attcagaaat gggcacaccc agaggcacaa
     6481 atcatggaaa aatgtttcct gcaggtactc ctctgcaggt gatggtagtt gaggttaatc
     6541 aggatcgggg caggataaca ttgagcagaa gcggcgttga agagagactg gaaaaggagg
     6601 agcttaccag ttacatgaaa caggcccagc aggatgaaaa ggaaagcagg agtctcggca
     6661 gctttggtga gctgctcaag gctgctatgg ataaaaaagc aagtaaaaag ggcgagctga
     6721 ccagccagaa atagatcgat taaacggcac ggcgcagctt ggctttaggg ctttctctca
     6781 aaaaagtttc cccatgggca gccttgaatg gtttctgctc cttggcacta ccctggcctt
     6841 tcttgttttg ctcgataaca gccttacatt ccggacatag aatacctgta tcattctgac
     6901 gtaccttgaa aatactctgg catttatagc agacttcttc attgcttttt ctacgtggat
     6961 aatcaggata gctccggaaa aggcatgtag gtcgcgaaca tacaagcacc ctcaccctgt
     7021 tgatgtggtc atattcttca aacatctcag aatcacaatg cgggcagttt cgtgccattt
     7081 tatcacccct ttgaggcgtt ctgttttcaa gatttatcgg tatatttgct atttttttaa
     7141 taccttttaa tagaattgcg aaacacagga atatcaaaac aacaggaata cttggccttt
     7201 tgggaaagcg aggcagaccc agcatacagg caagaacaca ggatcaatgg gttaggatta
     7261 tcaacctcat tcccgggcat gagatggagg ttctgccccg ttgtcgcggt atggaatgag
     7321 tagccaagct gccgaagcct cggtgcaggc aaaggcaaga gccactaaac tgaaaagagt
     7381 tccccatcga ccattttcat ttttctttca ccaatggacc cgatctctgg gtcatgggtt
     7441 accagcacga gggttgatga caatcgatca ttgattgaaa ggaggtagtc aagtacctcc
     7501 ttgcctgtct tacggtcaag attaccggta ggctcatctg ccagaataat ttcaggctcg
     7561 ttgacaagag cccgcgcaat ggcgacccgt tgttgttctc cacccgataa ctcacccggt
     7621 ttgtgttccc tcctgtgctg caatcccatt atttccagaa aactttcagc tttcgcaaca
     7681 gcatcattgc gacttaagcg cctgataagg agaggcatta tgatattttc tataacattg
     7741 aaatcctgga gaagatgata aaactggaat acaaatccaa ccttttcatt cctgaatctg
     7801 ctctcctcat cttctgaaaa ctccttgata tttttgtctc gaaaaaaaat atctccctca
     7861 gacggcttgt caagggtccc aaggatatgg aggaaagtgc tctttcctgc cccagaaggg
     7921 cccatgatgg ttataaagtc gccttcattg atatcgacac tgattccctt gatcacattt
     7981 atctccacgc cgtttttgct gaaggttttt tttagtccgg acacacgcag aattggctca
     8041 ctcataccgc aacgtctcca cagggtcaat ttttgaagcc ttataagaag gatagagcgt
     8101 ggcgagaata caaatgatcg tcgtaatcga tgccaccagg aaagtatcaa gaatgcttat
     8161 tttgattggg agggtgttaa tgtaataaat atctgagtca agtttgacaa tagagaacct
     8221 tctttgaatc tcgcacagga tgtacccact gaaagaccct atgagggcgc cgaagattcc
     8281 tatggttata ccttccacca taaagatttt catgaggctt gccttttttg caccaatagc
     8341 tttcaggata gcaatatcct tttttttctc catgacagtc atgatgagag agctgataat
     8401 gttgaaactc gccacgaaaa taatcagggc aaggataata aacatggcaa tcttctcaag
     8461 cttaagggct gaaaaaaggt tcctgttcat ctcgatccat gtcctggcaa aaaaattggt
     8521 tgcggagcca agacgggtca tgatctcttt tcggatgcta tctgcctggt aaacatcatc
     8581 aattttgatc tcgatacctg taacaccctg tcccatgatt gcttcaatat cctggatcga
     8641 cattatgatg agtgagttat caatatcata gacacctgtc tcaaatacac cgccaacttt
     8701 aacgcgcacg gtttcgggaa ccgctcccat aggagaaaca ccaccgaaag ggaccatgat
     8761 tgtcagaaag tctccataaa gcacgccaag atgttttgaa agctctttgc cgatgagtat
     8821 atttccacgc tgattaaggg ccccaatgct gccctgcttt agcagacggg acatgaactg
     8881 aacatctcct ggattgagcc cttttaccag tgctcccgat aattgctttc ctgtctggac
     8941 catagcctga aaggaaataa agggaaaagc atgggttacc ccttttatat ccttcaccgc
     9001 ggatacaacc ttttcaggat cctttatgtc cccagccaga ccgagtataa gcacatgtgg
     9061 attaatacca aggatacgtt ctctgatctc attctgaaat cctgtcatca cggcaatgac
     9121 caggatgagg gccattactc caatggcaat accagcgatc gatatccaag ttgtaaagga
     9181 aatgaatgct tctttccttt ttgaacgtaa ataacgtaat ccaactgtgg tttcatagtt
     9241 catctgccta acccccgaga cgcgaggccc aagacgcgtg aagggttttt attgcttcat
     9301 tatggttcac tgcttctatc atggcctcaa caatgggaaa agtattacgt ctcgaattga
     9361 gggactgtca gtcaaaagca ttaccagcct gtcgatccct ataccttccc cagccgtggg
     9421 agggagtcca tactccaggg ctgccaaata atcttcatcc agaacagccc cctcttccct
     9481 atcctttacc tgttccataa atcgttgcct ctgatcttca gggtcgttga gctcgttgaa
     9541 gccattggca atctccatcc cggcaatgta aagctcgaaa cgctcagtaa catcgggttg
     9601 gtctttgctc cgttttgcaa ggggagacac ttccactgga tagtgtgtaa taaaagttgg
     9661 ctgaataagc ttctcctcac agagagtctc aaaaattttt gtaataatct tgcccttcgc
     9721 atctttctct acaccttcta tctcaaggct ctttgcatat gatgtcaggg tgcccagatc
     9781 atgggatttc tgcagatcaa atcctcccag gtcccgcatg gcttcttcca tggtcatacg
     9841 tttccatgga gcagtgaaat caatctcctg gccgttgaag acaatctttg ttctcccaga
     9901 gagcttcaca gcaagggaag aaacaaggtc ttcggtaaat gtcataaggt cttcatatgt
     9961 tgcgtaagcc tgatagaatt caagcattgt gaattcaggg ttgtgtctta ctgatattcc
    10021 ctcatttctg aagttccggt ttatctcaaa aaccctttca agaccgccaa caataagtct
    10081 tttcagataa agctcaggtg ctattctcaa atacaggtcc atacccaggg cattgtgatg
    10141 tgtaataaaa ggcttggcaa ctgcaccgcc aggcagtacc tgcatcatgg gtgtttcaac
    10201 ctccataaaa tcccgggatg caaaaaaatt tctaatatat tcgataatta tagctctctc
    10261 ctcaaacacc ttccgtgact tttcattcac gatgagatca agatatcttt ttctgtatcg
    10321 ctcttctata tctttaagac catgccattt ctcaggcagg ggtctcaggc tttttgtgag
    10381 gagactaatc cgttccgcaa gaatcgtcag ttcctcggtc tttgttttga atatttttcc
    10441 ctctgcagag atgatgtccc caatatcaaa cttcttgaaa gcctcgtaag gaaggccctt
    10501 gagcatatct tttcgtacat atacctgaat cctgcccttc ttatcctgaa tatggagaaa
    10561 agcgctcttt ccaaaatccc tgaaggctac aatccgtcca gcaacagaga gggtggctcc
    10621 ctctttttcc agatcatcgt ggctcatagc gccaaaacgc tgggcaatgc ttgctgtatc
    10681 aacataggat cccctgccct gggggtatgt gtctatgcca atactccgaa agtatttttc
    10741 tttatctttt ctgactgcta ccaactcatt gatcggttcc atataatcat cctttcaatt
    10801 tacaggcact catacttgcc tacgcaaagg ctttggaaga caggtcgctc tctacccaac
    10861 catttaaacc gttacagcaa acccccttca tgcccacaaa agggctggct ctccaggctt
    10921 atggtatatg tatctttgtt tttctgcacc atgctctcag cctttagctg ctctttccac
    10981 tatggcttgc ctttcaatat ttcaggtttt tcctgttttc ccttatgttt cacctttcag
    11041 ttcttactcc ttttcgtgcc tttcttgctc agctgaaata tcggggctat accaaaggaa
    11101 tctacacctc aactataaca tcataatcga gggttttcac tattttccct tgtcgaattt
    11161 ctccgggagc acattttcct ttgataaatg ccatgccgtc aatatccggt gcctgtgtta
    11221 taatccttcc tgtcatgtaa ggtttttcat agcctccaac gatcacaaag ttctttgtgc
    11281 ctacaagacg tccgagtctt cttcctgaaa tttcccgctg catttccata atcgtgttgt
    11341 accgcttttg ttttgcattt tttctaagat ggcccttaaa cctgaatgcc cttgttccct
    11401 cttcccgtga ataggtaaag gcaccaagca tatcaaattc gcattttttt acaaaatcac
    11461 agaggccagc aaactcgttc tctgtttctc ctggaaaacc tactagcacg gttgttctca
    11521 gagtggcgtt tggtattcta tcttttatct tttgaaaaag acaatcgagt tgttttctcg
    11581 tatagcccct gcccatcagg ctgagaatcc ggtcctcaga atgctggatt gggatatcaa
    11641 ggtacggtat gatcttctca ctctccccta tcaggtcaat gagctcatcg tccacgccct
    11701 tcggatgcat gtaaagaagt cttagaaaaa aatctccctc aacctttaac atatgacgaa
    11761 gtaaagtctt gagatcgaac ttcccatcca tgccatagga tgtgatgtct tgtccgacaa
    11821 tgctaatttc tctatagcca ttctccagaa gatatgtgaa ttctgcccct atgtctgcga
    11881 tcgttcggct tcggagatca ccccttatac tcgggatcgt acagtaggcg catctgttgt
    11941 tgcaaccctc ctgtatcttc aaatatgcag agggcttgcc ggtaagcacc ttcctggggt
    12001 aactctcata aaaaggtgta tcttgaaaaa aatagcctgc ttcagtgata actttgtcta
    12061 tctcatgata catacccctc ccaacgaata gatctacttc aggcagcaag tttacaagct
    12121 cttcccggta tcgctccaca aggcaaccag ttacaactat ctttctttct ttcttatttc
    12181 gttcttttgc cgccatcagg atagtttcaa tagactcttg agccgcatct cggatgaagg
    12241 cacaggtatt aataatgacc gtattgcaat catcggagag aatatgcccg tccttttcca
    12301 gcctgaaaga aatatactcc gattcaacaa gatttttcgg gcagcccaaa ctgatgattt
    12361 tgaatttcat cgcttttcaa ctattttgca gaagcaagca tttttcaata cagtcagtcg
    12421 ttttcactcc agtacactta ccattaatcc ctacactctg acagagaaag aaatcatatc
    12481 gaagggggtc atcgggcgag aaagacttca atacatcggt aatttcacac gcagctttcc
    12541 aggaagggct tttcagccgc gtccatccaa aacatttgcc aatcttgaag atgtgcgtgt
    12601 caagggggat aaccagccta ttctttggaa taaagtccca taatcctaca tcgatctcat
    12661 cttttctcac catccaccgg agatacaggc tccacctctt catgggattc gattcagaag
    12721 gttttgggaa aaagaaggtc aatctctcat cgctccctat aagctcatca cgtatctgcc
    12781 atatcatttt tcttgtatcg cccgtataaa aattttgaag cattgccccc atactgtcaa
    12841 aatactcaag gatccgtctc agaataataa acagatatac aatgtcatca gccttttgaa
    12901 acctgtaata gaggcccttt aaggaggaga aatctccttc catcacaaat ttccacggtg
    12961 actcgcccat gagagaaaac agtttttcta aaaactgtct tagaacaccc accttgccgt
    13021 atgcaaattg ggaactgaga aaacctgcca actccttgtc ttttgaagat ttgaaacgat
    13081 gaagaaactc aaccggatca tttctgacca aaggatggag attcgatttt tccctactgt
    13141 atatttcgct gagattgatg tgttttcctt ttcctgagtg ttatctggga aactgccttt
    13201 gtatttatct gctttcggtt gctgcccttg ctttattttt taattctttc attgtagccc
    13261 caaaggcttc gctggctctc ctgacctctt cgtcccattc atcccaggca tcgagattgg
    13321 gctcaatagc acttcctgta atatttaatt cggcaagggc atccctgatc cttttctgga
    13381 ttgcagttct cctggattct atctcttcac gaaatttgcg atttattgtt tcaagatcac
    13441 tacgaatgga ctgtggcagg tctattcctg cagcatgaaa aatggcaaat aatcgttcgt
    13501 tgtctttatg aacatcaaac tcatctatca gattttttat gattatagta cgttttgatg
    13561 acatgtcgcc ttgaattttg cccagctcct ttgcagcacc ttcgtggtct atcattgact
    13621 cagcaaacct tctcaggact gcctttactt tatcttctat ttccttgatt gccatggcct
    13681 ccctttcttt gggatctacg accaggttct ttgttctttc cattgccagt tctattgcgc
    13741 tctttatctc tgccattctg ccgtgttata tcctccctgt gagcataagt ttcactgata
    13801 aaactctgtg taaggttgtc aatctgatca tactttattt tgcctattca gtaccggata
    13861 ttatgaaaag attataaagg cttcggagat tcaggtcaag tattgatgca agcactggaa
    13921 aatggtaaat tttctggtgc aaatcacaaa agcatataaa aggttttatc tttatcttct
    13981 aaaggaatta acctacatct tactcctttc ccttgccatt ctcaccttca ttcttgtcat
    14041 gagcaggctt ggaaaactca cagacctcgt catcaacaaa ggtgttgggt tgatggacat
    14101 tgtacttctt atcgtatatt cctcaccacc ttatcttaca tttacccttc ccatggcctt
    14161 tcttctatct accatagtga ccctggggag gctttccact gaaaacgaaa tattggcgct
    14221 caaggcaagt ggggtcaacc tgaggtgctt gtttgttcca atcgcatccg taggtatcat
    14281 catcagtgct gtggctattt ttaacacaaa ctttctgtta ccaacaagcg ccaatctttt
    14341 tcgtgaaaca ctgttgaatg ttctcaaaaa aggaatagct gttgatgaca aagaaggaat
    14401 atttaatgac agtataccag gagtggtgat ttacatagac agagtggata cacaaaccaa
    14461 tagactgacc ggtatattgg tttcagacga ccgcgacaag gatgagaaac agacaatttc
    14521 tgcctctacc ggttttatcc atcttgatcc agcaaccttg gacctctcat tcattctata
    14581 caacgggagt ttgcataaat gggaaaaaaa gagcgatact tacaggacag tatcattcaa
    14641 ggattacaat ttcatgatta atcttgcaaa catgcttcct ccaagcgccg gtatcaggaa
    14701 aagacccttt gaaatggaca ggtcagagtt gaaaaatgcc atgataaggg cgaccaaaga
    14761 tcgaaaatac gatttacaga tagagatcta taaaaagata accatacctt tatctgcgtt
    14821 ggcttttgtt ctcctcacag taccccttgg tatcaggagg aaggttgagg ggagattttc
    14881 cggggttgtc tatagtcttg ccctctttgt cttttattac atgcttatgg ctctcactga
    14941 aaatctggga agggcattat atgtgcctcc catatttatt gcagccatac caaacacaac
    15001 catagtcgtg ctcggccttt acctcctcag gaacctgaac gttgaagaac cggcaaaaac
    15061 atcacacctc atcagatcct tctggatgcg ataccatgaa aaggctaaat agatatctca
    15121 ttcttaacgt attaaaactc ctgctcctta cccagtttgc cggtcttgtt atgtttgttg
    15181 ccattgagtt cctggaacat atggatatct ttacggactc ccttaacagt ttcctgctca
    15241 gtcttgttta catcctatta aggcttcccc actattttaa cctgctgctg ccattggcat
    15301 cccttatctc catgctcatc cttctgattc tgatggtgcg aaataatgag atcatcgctg
    15361 caagaacatc gggtataagc actatctccc tgatgaaacc cttcattgct tgcgcttgcg
    15421 ggctcattat ctttaccttt attattgccg agtggatcat gccttatgct gcaaatgctt
    15481 ccgaatacct gtatagagta aaaatcaaaa aagaagactc ttacattcat tttaaaaatg
    15541 acaggatatg gttcaagaga aacaatgtgc tttacaacat cgattattat gatatgaaga
    15601 aagatgttat tagaggactc acagttatag aacttgctga cgatttcact attcaaaaaa
    15661 ggattgatgc caaagagggg atatggaagg attcccagtg gg
//
LOCUS       DAOOMC010000067        15520 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdus sp. isolate AMR_MDS_2685
            NODE_4039_length_15520_cov_5.817717, whole genome shotgun sequence.
ACCESSION   DAOOMC010000067 DAOOMC010000000
VERSION     DAOOMC010000067.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864760
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdus sp. (wastewater metagenome)
  ORGANISM  Syntrophorhabdus sp.
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae; Syntrophorhabdus.
REFERENCE   1  (bases 1 to 15520)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15520)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,729
            Genes (coding)                    :: 2,708
            CDSs (with protein)               :: 2,708
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 7 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15520
                     /organism="Syntrophorhabdus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4039_length_15520_cov_5.817717"
                     /isolate="AMR_MDS_2685"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2699756"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            290..913
                     /locus_tag="PK125_08105"
     CDS             290..913
                     /locus_tag="PK125_08105"
                     /inference="COORDINATES: protein motif:HMM:NF012780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HPB38109.1"
                     /translation="MTKNVITITSDTYVLDAERIMDQNRIGRLPVVDDGKLVGIVTRN
                     DVLKASPTSTTPTNQRHLFYLMSKLTVKEIMKTKILKISPDTPIEKAIAVAQKNKVGS
                     LPVVEEDKVVGILTTNDVFYKILNPLFGIGQKGSRIVIHGAGKVDMMQEILEFIRKTG
                     LEVKTYWIPPSDDRQDLFLHINIEDATQIIEELRGRGYSVEKREFSV"
     gene            complement(971..1837)
                     /locus_tag="PK125_08110"
     CDS             complement(971..1837)
                     /locus_tag="PK125_08110"
                     /inference="COORDINATES: protein motif:HMM:NF015325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HPB38110.1"
                     /translation="MEKNTLDSDTKGEARKGKLILGLIGSPRKLGNCEVFVKEIALSV
                     AEDCKLKLIRMPSLTIEQCLACYRCIMDQPCPQKDDMESLLQQIVHSDAIIIASPVYF
                     LGSHSIIKRIIDRGFLFYEVLEKTYGKPCILINFYGMKNRIGVAPHTMMSFAAFLGLN
                     IKANVNVKAALPGEVLVSPVQRNLAKKLARALFKEGNTSKNHGCPFCGNDIVRMEKRK
                     FTCTLCHGAFRVDDRGERIKIRDGGILGPPEHMLLHKSWLRGMKEEFMKRRKEILRLT
                     QQYKDIGEWIRL"
     gene            1968..3803
                     /gene="ftsH"
                     /locus_tag="PK125_08115"
     CDS             1968..3803
                     /gene="ftsH"
                     /locus_tag="PK125_08115"
                     /inference="COORDINATES: protein motif:HMM:TIGR01241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HPB38111.1"
                     /translation="MPKNPTLNPMNKNIFVWLFIILFGLFIFNIYYKPKKNYESVIFS
                     DFVEAVQAEKVTSVTVQGRNIIGVFKDGKEFKSYAPDDPDLMKLLRTHNVKINAKPDE
                     EGGLWQNLVISWFPMLLLIGVWIFFMRQMQAGGGKAMAFGKSKARLITGKENKVTFQD
                     VAGIDEAREELEEIIDFLKDPKKFTKLGGRIPKGVLLVGPPGTGKTLLAKAIAGEADV
                     PFFSISGSDFVEMFVGVGASRVRDLFNQGKKQAPCIIFIDEIDAVGRHRGAGLGGGHD
                     EREQTLNQLLVEMDGFESNEGVIVMSATNRPDVLDPALLRPGRFDRQIVVSIPDVKGR
                     EAILKVHTRKNILSNNVDLSIIARGTPGFSGADLENLVNEAALLAARKNKKEIEMEDF
                     EHAKDKVLMGVERRSMIIPYQERRNAAYHEAGHALVAKMIPGSDPIHKVTIIPRGRAL
                     GITQQLPIDERHTYSKDYLLDNITILLGGRVAEELVLNHQTTGAGNDIERATELARKM
                     ICEWGMSDRLGPLNYGKREEHIFLGKEIAQHKNYSERTAQEIDEELQKTVNGCFDRAR
                     QLVLANMDALHAIANKLLEKEVLDGQEIDGIIKENTNGKGIAVEG"
     gene            3823..4596
                     /gene="folP"
                     /locus_tag="PK125_08120"
     CDS             3823..4596
                     /gene="folP"
                     /locus_tag="PK125_08120"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR01496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HPB38112.1"
                     /translation="MNVTPDSFFDGGKYSTRVKAVDHALRMAEDGADIIDVGGESSRP
                     FSKPITIDEELRRVIPVVEGIRERLNIPISVDTCKARVAQESFFAGADMVNDISGLRH
                     DPEMADTVNAAKAKVIIMHMKGTPQTMQIDPSYDDIIFEICAFFEERIDFAVTGKGIE
                     RSNIVLDPGIGFGKRVEDNLRIIKHINIFKKFNLPVLVGTSMKGFIGKITESTQRGRL
                     EGTLASIALSVWNGADIVRVHDVKKAKKTVVFTDAVMRV"
     gene            4647..5387
                     /gene="cdaA"
                     /locus_tag="PK125_08125"
     CDS             4647..5387
                     /gene="cdaA"
                     /locus_tag="PK125_08125"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: protein motif:HMM:TIGR00159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase CdaA"
                     /protein_id="HPB38113.1"
                     /translation="MLSGLRWQDVLDILAVSFIIYRTFLLIKGTRAIQLIVGFIIVFF
                     VFYVSKKLELFTLGWIFSNFVGAIVFVIVVIFQNEIRRVLLAIGRSPFFKRITYVEET
                     LFYDELLNACSVMSSRRIGSLIVIEREVGLEEFMEIGIRIDAAVNTELIVSMFQTTSP
                     LHDGAMIIQEGRIRAAGCILPLTLKDTVDISLGTRHRAAIGITEVTDALAVVVSEEKG
                     RIAYAYKGEMFTNISEEALKKVLKELLR"
     gene            5440..6081
                     /locus_tag="PK125_08130"
     CDS             5440..6081
                     /locus_tag="PK125_08130"
                     /inference="COORDINATES: protein motif:HMM:NF019561.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CdaR family protein"
                     /protein_id="HPB38114.1"
                     /translation="MDFVKRYILNDWKLKGLSLVLAIMLWFAVSYVGESKLSVSVPVL
                     PANLEKDFMIKNMDVEGVLVMISGPVSVLKNLSPKDIKVPLDMGELKNGRHIINIEKA
                     DIVVPKGVQIEAVKPDYVVIEVERTMEKRLKTIVKLDDKWKGLYNVKSWYPQFVTVEG
                     SKASLEKAETIQTVPIDGDFTAAEEELDIALDTQGLFVRKLRPETIRVILKRH"
     gene            6097..6813
                     /locus_tag="PK125_08135"
     CDS             6097..6813
                     /locus_tag="PK125_08135"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006762396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HPB38115.1"
                     /translation="MGSLMVNIDHVATLREARGINYPEPVYAAGIAEMAGASGIIVHL
                     REDRRHIKDRDVRLLREVVRTKLNLEMAATPEMVQIATNIKPDMVTLVPEKRQELTTE
                     GGLDVVGQSGKLRKTVESLRSAGIFVSLFIDPSEEQLVASQKIHADMIEIHTGTYSDA
                     VNEKIKEEELKKIARSAMRAKELGLGVNAGHGLHYHNVKEVAAIREIDELSIGHSIIA
                     RAVFVGLDMAVRSMIELINQ"
     gene            6901..7221
                     /gene="acpS"
                     /locus_tag="PK125_08140"
     CDS             6901..7221
                     /gene="acpS"
                     /locus_tag="PK125_08140"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HPB38116.1"
                     /translation="MVGIDIVDVSRMEGIVKRFGDRFLHRVFTGQELEYAGKKVRIQE
                     TLSGRFAAKEAFMKAIGESAGWKEIEVLQQGGKPYIVFRGKVYREVSISHERRFAVAV
                     VVIR"
     gene            7326..8900
                     /locus_tag="PK125_08145"
     CDS             7326..8900
                     /locus_tag="PK125_08145"
                     /inference="COORDINATES: protein motif:HMM:TIGR00196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HPB38117.1"
                     /translation="MVVLTPERMAEYDRYAINTLGIPSAVLMENAGRGTYRLAKERYF
                     SGKEKVTIFCGRGNNGGDGFVIGRYALRDGFQTRVFLLCKASDLKGDAALNMGIYKSI
                     GGKISECTDKTKGITDGIRDADIIIDAIFGTGLSKPVSGIEKAVIEDINISGKPVIAV
                     DIPSGIDGKTGQPLGTAIRATHTFTYGYPKIGQILQPGAYHTGKLTVIDISMPSLIEG
                     KLGFDGRVIEGAMVRGFLKKRLPWDHKGTFGHVAVIAGSPGKTGAACMTSQAALKIGA
                     GLVTLLIPESLNSIVASKLSEVMTYPVRDRGTGYFHLSAYKEISDFVSDKDVIVMGPG
                     LSQESDTTMLVRELYQNINKPFVIDADGINAFQGQTDIIKGSGRKAVFTPHPGELSRL
                     MGVTPKDINNDRVGVAQRFVRHTGANLILKGARTVIVSDKEELFINPTGNPALAKGGS
                     GDILTGLVGGLLSQGHAPIEASIIGTYLHGYIADTWVQNNTDMDLLAGDLVSGIGKAL
                     RDIRDGKDRVYIEKSL"
     gene            8863..9282
                     /gene="tsaE"
                     /locus_tag="PK125_08150"
     CDS             8863..9282
                     /gene="tsaE"
                     /locus_tag="PK125_08150"
                     /inference="COORDINATES: protein motif:HMM:TIGR00150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HPB38118.1"
                     /translation="MARIEFISRSPSETWDIGEQVGSAARRGDLYAIYGELGAGKTQF
                     VKGVAKGLGVEDWLYVVSPTFTIMNIYEGKDTKLCHVDLYRIEENDFAPLGIEEFLEE
                     GVVAVEWAERACWWDGIIRVDIEIIGEEERRIRITKT"
     gene            9357..10595
                     /locus_tag="PK125_08155"
     CDS             9357..10595
                     /locus_tag="PK125_08155"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: protein motif:HMM:NF005155.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HPB38119.1"
                     /translation="MLVVQKYGGTSVADVDRIRNVAKRVISYREKGDKVVVVLSAMAG
                     ETDKLLNLAYSIDKFPDEREVDVLISTGEQVSSALLAIMLKNMLCDAVSMQGHQVRIV
                     TDSSYTKARIATIEKDRLAAELDKGRVVVVSGFQGIDEKGDITTLGRGGSDTTAVALA
                     AALQADICEIYTDVDGVYTTDPSVCHKARRLDKISYDEMLEMASLGAKVLQIRSVELA
                     KKYNVPILVKTSFGDGPGTLVCKEGATMSMEKVVIAGVTYNKNEAKIVVRKVPDKPGM
                     AAKIFTALSDAKIVVDVIVQNVSKKDKTDITFTVSRTDAKKAYKIMEEMAKKIGAAGV
                     SIDEDIAKVSIVGSGMISHAGIASKMFAVLADEGINIKAITTSEIKLSCVVESKYGQL
                     AVQSLHKAFGLDKEEIKEEM"
     gene            10592..12163
                     /gene="cimA"
                     /locus_tag="PK125_08160"
     CDS             10592..12163
                     /gene="cimA"
                     /locus_tag="PK125_08160"
                     /inference="COORDINATES: protein motif:HMM:TIGR00977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043714 - (R)-citramalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citramalate synthase"
                     /protein_id="HPB38120.1"
                     /translation="MTVEFYDTTLRDGAQSEDIAFSLTDKLRITEKLDEFGMHFIEGG
                     WPGSNPKDLSYFKDVKRLNLKNSKIVAFSSTMKPHSKPEEDEIIKAIFDADTEFVAIV
                     GKSWDLHVIDALKVSLDMNLRMIDRTITFLKKHGKKVFFDAEHFFDGYKKNNAYAMKV
                     LEVAVNSGADVVVLCDTNGGSMPFEVSDIVAKVTNMTEAKLGIHTHNDTENAVANSLM
                     AVKAGCRHVQGTINGYGERCGNANLCSIIPNVVLKMGHRGIKPERLIHLRELSLFVDE
                     MANLLPNKHQPFVGDSAFAHKGGIHVSAIRRNPETYEHIKPELVGNAQRVLISDLSGE
                     SSILYKAKEFNVDIEKDKKLAREVMKNVKDLEMYGYQFEGAEGSLELLIKKALGIHKK
                     YFDLIGFRVMVEKKEKNHPVSEATILVRVADTVEHTAALGAGPVNALDNALRKALHKF
                     YPKLTEMNLVDYKVRVLSTRDGTKAATRVLIQSSDGKQTWGTVGVSENIIEASWQALV
                     DGIDYKLLIEEEKDR"
     gene            12160..12624
                     /locus_tag="PK125_08165"
     CDS             12160..12624
                     /locus_tag="PK125_08165"
                     /inference="COORDINATES: protein motif:HMM:NF020091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-isopropylmalate synthase regulatory
                     domain-containing protein"
                     /protein_id="HPB38121.1"
                     /translation="MKKLLNIRDATKELNILRSLRNYKRPFEIVGAYRLIDDGSRPEA
                     TVIIKRNDHETHEASTGVGPVDALANVLKKSLASIFPVIQQIKLVDYSSRIHEAKSGT
                     AAQVEVSIIFTDGQEIWSVAAISANINMGSFLALLDGFEYAILARSEKKQSL"
     gene            12803..14509
                     /locus_tag="PK125_08170"
     CDS             12803..14509
                     /locus_tag="PK125_08170"
                     /inference="COORDINATES: protein motif:HMM:TIGR03710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HPB38122.1"
                     /translation="MKIGGEAGQGIQTIGDTLSRVFSRCGYHVFTHQDFMSRVRGGHN
                     FYQMRVSDKEISASRGPVDILVALDSDSISSHSKELSAIGVVVYDSAALKEKHDGSQF
                     FDVPLLDLALKNGGDKIMVNTVATGTVLGMLGLGTDIFLDILREILGKKGEAVLSANA
                     NAARAGHEFAGRECLRCEFSTPAIGTKKMLIGINEAVGLGALASGCKFYSAYPMTPST
                     GIMLYIASKAKEFEVIVEQAEDEIAAINMALGASFAGIRAMTGTSGGGFALMVEGLSL
                     AAMTETPIVIALAQRPGPATGLPTRTEQGDLLFALHAGHGEFPRVIFAPGTPEQAFLL
                     TNKAFDLAQKYQIPVFILIDQYLSDSQWTYDGFNLNKLTLTDWRATESELEGVKDYKR
                     HFLTDHGISPFARPGTSRHLVVTDSDEHDEDGHLIEDAATRVQMVDKRLFRKMPLIRS
                     EITPPVFYGNSRPDIILTGWGSTYGIMKEAVNILSETTKVAMLHFSEIFPFPSSQELD
                     YLEILGKANMTIIVEQNASSQFARLMKTETGFEFTGKINRYDGRPFLVEELLGEISAY
                     IG"
     gene            14493..15356
                     /locus_tag="PK125_08175"
     CDS             14493..15356
                     /locus_tag="PK125_08175"
                     /inference="COORDINATES: protein motif:HMM:TIGR02177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:ferredoxin oxidoreductase subunit
                     beta"
                     /protein_id="HPB38123.1"
                     /translation="MPTLDDYKGQEPAWCPGCGNFPILRAFKEAFVEMDIEPHQFLIV
                     SGIGQSGKFPHYLKCNTFNGLHGRTLPVATGARLANHEMLVVAVAGDGDCYGEGGNHL
                     MHAIRRNIGVKLFVHDNQIYGLTKGQASPTSMEGMVTKNQPFGVLSEQLNPMALAVAL
                     DCSFVARGYAGDHEHLKGLIKTAVNHKGFSLVDIFQPCVTFNRVNTYAWYSQRVYHIE
                     DDYNPEDRVAAFQKALEWGDRIPIGVIYRNNRPTFEERVPVINELTLVKQPVSIEGLK
                     SKLRKTIKEFF"
BASE COUNT         4526 a         3076 c         3916 g         4002 t
ORIGIN      
        1 atcaaagact gatatccccg gatgtgaggc acatatacac cctctctatt gaagaatgga
       61 tagggtgcag agggaattat aataatgggg aagtagagtt ttgtaatatt ataatcagtt
      121 tcaaatagca acataagtat ggaaaagatc tgtcttgtaa tagccaaaat ggtacgaggc
      181 tgaaagattg tatgcttaag aataaacctc ttgacaggta gactccaaaa ggtcacaata
      241 cttctgtagt attgctagaa aagaatagtc tatgctggta cgagatatta tgaccaaaaa
      301 tgtcatcacc atcacgagtg acacctatgt cctcgatgca gagaggatta tggatcagaa
      361 caggattggc cgtttgcctg ttgttgacga tgggaaattg gttggtattg taacaaggaa
      421 tgatgtgcta aaagcaagcc ctacatctac aacaccgact aatcagcgcc acctctttta
      481 cctcatgtcc aagttgacgg tgaaagagat tatgaaaacg aagattctga aaatatcccc
      541 tgatactccc atagaaaagg cgatcgctgt tgctcaaaag aacaaagtcg gcagtcttcc
      601 agtagtggag gaagataagg ttgtcggtat cttaacaacg aacgatgtgt tttataaaat
      661 actgaaccct ctctttggaa taggccaaaa aggtagtcgt atagtcattc atggcgccgg
      721 aaaggtagac atgatgcagg aaatactcga attcataagg aagactggtc ttgaagtgaa
      781 gacctattgg ataccgccca gtgatgacag gcaggacctg ttccttcaca taaatattga
      841 ggatgctacc cagatcattg aggaactccg gggcagaggg tactctgttg agaaacgtga
      901 gttttcggtg taaggtcatc ttcattaaga ctccggagat tgatttttac gtccaagggg
      961 tgcatttgtt ttatagtcta atccattcac caatgtcctt atactgttga gtgagtcgca
     1021 gaatctcctt gcgccttttc ataaactcct ccttcattcc ccttaaccat gatttgtgaa
     1081 gaagcatatg ttcgggtgga ccgaggatgc ctccgtccct tatcttgatt ctttctcccc
     1141 tatcatcaac tctaaaggcc ccatggcaga gtgtgcaggt gaattttctt ttttccatgc
     1201 gcacaatgtc atttccgcag aaagggcagc catggttctt ggatgtgtta ccctctttaa
     1261 aaagagcccg tgccagcttc ttcgccagat ttctttgcac ggggctcacc agaacctcac
     1321 caggcaacgc agccttcaca ttcacatttg ccttgatatt gaggccaaga aaagcggcaa
     1381 aactcatcat agtgtgaggg gccactccaa ttctgttttt cattccataa aaattaatga
     1441 gaatacatgg cttgccgtat gttttctcaa gaacctcgta gaacaggaat ccacgatcga
     1501 tgattctctt tattatgcta tggctcccga ggaaataaac cggcgatgcg atgataatgg
     1561 catcggaatg aacgatctgc tgaagcaggg attccatgtc atctttttgg gggcatggct
     1621 gatccatgat gcacctgtag catgcaaggc actgttcaat tgtcagagaa ggcatcctga
     1681 tcagtttcag tttgcagtct tcagctaccg aaagagcaat ttccttgacg aacacctcac
     1741 agtttccaag tttgcgggga gaacctatca ggccaagaat cagtttcccc tttcgtgctt
     1801 cgcctttcgt gtcagaatcc aatgtattct tttccattgt gcaccttcaa taatgggttt
     1861 atactaccat tataccagat tcaggttaat tttatagttg ctacatttaa tagaacaaat
     1921 cgtttaaata ggttgaaaat tcaagggttt tatggtaaga accatagatg ccaaaaaatc
     1981 ccacactgaa tccaatgaat aaaaatatat tcgtctggct ttttattatt ctgttcggtc
     2041 tttttatctt caatatatac tacaagccaa aaaaaaacta tgaaagcgtg attttcagtg
     2101 attttgtcga ggctgtacag gcggaaaagg tcacgtcggt aacagttcag ggtagaaaca
     2161 ttatcggtgt ttttaaggat ggcaaagagt tcaagagcta tgccccagac gaccctgact
     2221 taatgaagct tctgcggact cacaacgtaa agattaatgc gaaacctgat gaagaaggag
     2281 gtttatggca gaacctggtt atttcatggt tccccatgct tctgctaatt ggtgtttgga
     2341 tcttttttat gcgtcagatg caggctggtg gtggaaaggc gatggcattt gggaagagca
     2401 aagcgcgcct gattaccggc aaggagaata aggtcacttt ccaggatgtg gctggcattg
     2461 atgaagcgag ggaagaactg gaagagatca ttgatttcct taaagatccc aagaaattca
     2521 caaaactcgg agggcgtata cccaagggcg ttttgcttgt aggtccgcca ggaactggaa
     2581 agacacttct tgcaaaggct attgcaggtg aagctgatgt gccttttttt agcataagtg
     2641 gatcagactt tgtggagatg ttcgttggcg tgggcgcctc cagggttcga gacttgttca
     2701 accagggaaa aaagcaggcg ccttgcataa tattcataga cgagatagat gcagttggca
     2761 ggcacagagg cgctggtctt ggcggaggtc acgacgagcg cgagcagact ctgaatcagc
     2821 ttttggtgga aatggatggc ttcgaatcaa acgagggcgt tattgtcatg tcagccacaa
     2881 acaggcctga tgtccttgat ccggcccttt tgcgtcctgg gagatttgac cgtcagattg
     2941 tcgtatctat acctgatgtc aaaggacggg aagccattct caaggttcat acgcgaaaaa
     3001 atattctttc caataacgtt gacctttcaa ttatcgccag agggacaccg ggtttttctg
     3061 gtgccgacct tgaaaacctt gtaaatgagg cagctttgct cgcagcccgt aagaacaaaa
     3121 aagagattga gatggaagac tttgaacacg ccaaagacaa ggtgctcatg ggtgtagaga
     3181 gaaggagcat gatcattcct taccaggaga gaagaaatgc ggcatatcac gaggcaggac
     3241 atgccctggt ggcaaagatg atcccagggt ctgacccgat tcacaaagta acaatcatac
     3301 ccagaggcag ggctctgggt ataacccaac aactgcctat agacgaacga catacatatt
     3361 ccaaggacta cttgctcgac aatattacaa ttcttctcgg aggaagggtt gcagaggagc
     3421 tggttctcaa tcatcagact accggtgcgg gcaatgatat cgagagggca acagaattgg
     3481 cgagaaagat gatatgcgaa tggggtatga gcgatagact tggaccttta aactacggaa
     3541 agagagagga acacatcttt cttggcaagg agattgccca gcacaagaat tacagcgagc
     3601 gcactgctca ggagattgac gaggaattac agaaaactgt gaatggctgc tttgacagag
     3661 caaggcaact tgttcttgcc aacatggatg ccctccatgc catagcaaat aaattgctgg
     3721 agaaggaagt cctcgatgga caggagatag atggcatcat caaggagaat acaaacggga
     3781 agggaatcgc agtggaggga tagaccactc ataatggggg ttttgaacgt cacacccgac
     3841 tctttttttg atggggggaa atactcaaca cgggtaaaag cggtagatca cgcattacgg
     3901 atggccgaag atggagccga cataatcgat gtaggtggag aatcttcaag gcctttctcc
     3961 aaacctatta caatcgatga ggagctcaga cgggttattc ctgttgtgga aggtatcagg
     4021 gagagattga atatccccat ttctgttgat acctgcaagg ccagggttgc acaggaatca
     4081 ttttttgcag gagcagacat ggtaaacgac ataagtgggt tgaggcacga tcctgaaatg
     4141 gctgacacag tcaatgctgc aaaagctaag gtaatcatca tgcacatgaa agggacgccc
     4201 caaaccatgc agatcgatcc atcctatgat gatatcattt ttgaaatctg cgcatttttt
     4261 gaggaacgaa tagactttgc agtcaccggt aaaggtatag aacgaagcaa catagttctg
     4321 gaccctggca tcggtttcgg aaaaagggtg gaagacaacc tgaggatcat aaagcatata
     4381 aatatcttca aaaaatttaa tctgcccgtc cttgttggta cgtcaatgaa gggttttatt
     4441 ggaaaaatca ccgagtcaac ccaaagagga aggcttgagg gcacccttgc cagcatcgca
     4501 ctatcagtct ggaacggggc ggatatcgtt agggtgcatg atgtaaaaaa agcaaaaaaa
     4561 acagttgtat tcactgatgc tgtaatgagg gtgtgaaata aaaacccttt gctgcgcaat
     4621 aagcttcgat agaaggaaag attttcatgc tcagtgggct gcgttggcag gatgtccttg
     4681 atattttagc tgtctcattt attatctatc gtacctttct cctaattaaa ggcacaaggg
     4741 ctatccagct aattgttggc tttataatag tcttcttcgt tttttacgtt tcaaaaaaac
     4801 ttgaactttt tacgcttggg tggatattta gcaattttgt gggagcaata gtcttcgtga
     4861 ttgtagtcat attccagaat gagataagga gagtgctcct ggcaatagga agaagcccat
     4921 tttttaagag aattacctat gtggaggaaa cactatttta cgatgaattg ttgaatgcgt
     4981 gttctgtcat gagtagccgc cgcatcggtt ctttgattgt catcgaaagg gaagtaggcc
     5041 ttgaggaatt tatggagatt ggcattcgca ttgatgctgc agtaaataca gaattgatag
     5101 tcagcatgtt tcagaccaca tccccactcc acgatggggc tatgatcata caagagggaa
     5161 ggatccgtgc agcgggttgt attttgcccc ttactttaaa agataccgtt gatattagcc
     5221 ttggcacccg acacagggct gcaatcggga taacagaggt cacagacgcc cttgctgttg
     5281 ttgtttcaga ggaaaaaggg agaatagcct atgcttataa aggcgaaatg ttcaccaata
     5341 taagcgaaga ggctctgaag aaagtactga aagagctgtt gaggtgatgc gctaggccac
     5401 atcccctgca atctgctctg caaggtttta tgaaaatata tggattttgt aaaaagatat
     5461 attcttaatg actggaagct caaggggctt tcccttgtac ttgcaataat gctctggttc
     5521 gcagtgtcct atgtgggaga atcgaagctg agcgtttctg tgcctgtctt gcctgcaaac
     5581 ctggaaaagg atttcatgat aaaaaatatg gacgttgaag gtgtgcttgt gatgattagc
     5641 ggtcctgtat ctgtcctcaa aaacctcagt cccaaagaca taaaggttcc ccttgatatg
     5701 ggcgagctca agaatggccg ccatataatc aatatagaaa aggctgacat cgttgtgccc
     5761 aagggtgtcc agattgaggc tgtcaaaccg gattatgtgg tgatcgaagt agaaaggact
     5821 atggagaaac gattgaaaac tattgtaaaa ttagatgata aatggaaagg tctgtacaat
     5881 gtgaagtctt ggtaccctca gttcgtcact gtcgaagggt ccaaggcatc cttggagaaa
     5941 gcagaaacta tccagacggt tccaatcgat ggtgatttca cggctgctga ggaggagctg
     6001 gatatcgccc ttgacactca gggattattt gtccgcaaac taaggccgga gacgattcgg
     6061 gtgatcttga agagacattg acatggagga aataaaatgg gttcgttaat ggtgaacatt
     6121 gatcacgtgg caacattaag ggaagcacga ggcattaatt atcctgagcc tgtctatgca
     6181 gcaggcattg cagagatggc aggagcctca ggaatcatag ttcatctgag agaggatagg
     6241 cgtcatatta aggatcgcga tgttcgactt ttgagagagg ttgtcaggac aaagttaaac
     6301 ctggaaatgg cagcaacacc tgagatggta cagatagcta ccaatatcaa gccagatatg
     6361 gtaacccttg tacctgagaa gcgccaggag cttaccacag agggtgggct cgatgttgtc
     6421 gggcagtccg gtaagcttag aaaaactgtt gaaagcctac gatctgcagg catctttgtc
     6481 agccttttta ttgatccaag cgaggaacag cttgtggctt cacaaaaaat ccacgctgat
     6541 atgatcgaaa tccacacggg aacgtatagc gatgctgtca acgaaaagat aaaagaagaa
     6601 gaactaaaaa aaattgccag atctgccatg agagctaaag agcttggctt gggagtgaat
     6661 gcagggcatg gccttcacta ccataacgtt aaagaagtag ctgctatcag ggaaattgat
     6721 gagttgagta ttggacattc aattattgcc cgggcagtat ttgttggatt ggacatggct
     6781 gtaaggagta tgattgagct gataaatcaa tagtgtttgg ctcaaggtca aggtgtactg
     6841 agggatgagg aaaacttaca cctgagagct aaaaagtgaa cagcgtcagg ggaactgacc
     6901 atggttggca tagatattgt agatgtgtcg agaatggaag gaattgtgaa gcgatttggc
     6961 gaccgcttct tgcacagggt ttttacaggt caggagcttg aatatgcggg caaaaaggta
     7021 cggatacagg agactttgtc cggacgtttt gcagcaaaag aggcattcat gaaagccatc
     7081 ggagaatcag caggctggaa ggagatcgag gtacttcaac agggcgggaa accctatata
     7141 gtattcaggg gtaaggttta tcgtgaagta agcatttccc acgaacgaag attcgccgtg
     7201 gcagtagtgg tgatacgttg acattgatga gtcggaatcc tggttatctg tcacaggtac
     7261 ttgtgctccc tctcaactca aacacgaaat gataggctcc aaacggttta atttcggagg
     7321 tacgcatggt tgttttgacg cctgaacgca tggccgaata tgataggtat gctataaata
     7381 cattggggat accctctgct gtgctcatgg aaaatgcagg aagaggcacc tacaggttgg
     7441 caaaagaacg ttatttttcc ggaaaagaaa aggtgacaat cttctgtgga aggggaaata
     7501 atggcggtga cggttttgtg atcggccgtt atgccctgag ggatggcttt caaacgaggg
     7561 tttttctcct ttgcaaagct tcagacctca aaggtgatgc agcccttaac atgggaattt
     7621 acaagtcaat cggcggtaag atctccgagt gtactgataa gacaaaaggg atcacagatg
     7681 gcatcagaga tgcagacata attattgatg ctatctttgg tacaggcctt tcaaaaccag
     7741 tgagcgggat tgaaaaggca gtgattgaag atattaacat atcaggaaaa ccagtaattg
     7801 cagttgacat tccctcaggt atcgacggaa aaacaggcca accacttggt actgctattc
     7861 gtgcaaccca tacctttact tacggctatc caaaaatcgg acagatactc cagccagggg
     7921 catatcatac gggaaaatta acggtcatag atatttccat gccttctttg attgagggaa
     7981 aattaggttt tgatggtcgt gttatcgagg gggctatggt tcgaggattt ctcaaaaaac
     8041 gtttgccttg ggaccacaag ggcacatttg gtcatgtggc agtgattgca ggttcaccgg
     8101 gcaagacagg cgctgcatgc atgacatcac aagcagccct aaaaatcggt gctggtcttg
     8161 taacgcttct tatacctgaa agccttaaca gcattgttgc ctcgaagctt tcagaggtaa
     8221 tgacctatcc ggtcagagac agaggaactg gttattttca cctgtcagct tataaagaaa
     8281 tctctgactt tgtcagtgac aaggatgtca ttgtcatggg gccggggctt tctcaggagt
     8341 ctgatactac gatgctcgtg agggagcttt atcaaaacat aaataaacca tttgtcatag
     8401 acgccgatgg aattaatgca tttcaaggcc aaacagatat tatcaaggga tcaggaagga
     8461 aagcagtgtt tacacctcat cctggtgagc tttcccgtct gatgggagtg acaccgaaag
     8521 atataaacaa tgatcgtgta ggggtagcac agcgatttgt tcgtcataca ggggcaaacc
     8581 ttatcttgaa gggtgcgcgg acggttatcg tgagcgataa agaggagctc tttattaacc
     8641 caaccggcaa tcctgccctt gccaaaggcg gcagcggtga cattctgaca ggtcttgtag
     8701 gtggcctcct ctctcaaggg catgccccga ttgaggcgtc cattataggg acctatcttc
     8761 acggatatat tgcagacacg tgggttcaaa acaacaccga tatggatctc ctcgctggag
     8821 accttgtaag tggtataggc aaagctttac gagatatcag agatggcaag gatagagttt
     8881 atatcgagaa gtccctctga gacctgggac attggagagc aggtcggcag tgcagcaagg
     8941 cgtggtgatt tatatgcaat ttacggagaa cttggggcag gaaaaaccca gtttgtcaaa
     9001 ggtgtggcaa aaggcttagg ggtagaagac tggttatatg ttgtaagtcc cacttttacc
     9061 attatgaata tttacgaggg aaaagataca aagctctgtc atgttgatct ctaccgaatt
     9121 gaggaaaatg attttgcgcc cctcggcata gaggagtttc ttgaagaagg tgtagtagct
     9181 gttgagtggg cggaaagagc gtgctggtgg gatggtataa tacgggtaga tatcgagatt
     9241 attggagaag aagaacgcag gattaggata acaaaaacat aggttgttca cggtttgcaa
     9301 tgggcggtta acgatgagcc gtgaacgtct tcaatattac aatggaggtc aaaaacatgc
     9361 ttgttgttca gaaatacggc ggcacttcag tagcagatgt tgacaggatc agaaatgtag
     9421 ccaaacgggt aatatcttat agggaaaaag gcgacaaggt tgttgttgtg ctgtctgcta
     9481 tggccggaga gaccgacaaa ttactcaatc tggcatacag catagacaag tttcccgacg
     9541 aaagggaggt agatgtactt atatcaacag gtgaacaagt tagttcagcc ctgttggcaa
     9601 tcatgttgaa aaacatgctc tgtgatgctg tttcaatgca aggccatcag gttcgtattg
     9661 taactgattc gagctacacg aaagcacgga ttgcaactat agaaaaagat cgccttgccg
     9721 cggagcttga taaaggaagg gtggtcgttg tctcagggtt tcagggaatt gatgagaaag
     9781 gcgatatcac aacccttggg agaggtggat ctgacacaac tgcagtggcc cttgcagcag
     9841 cccttcaggc tgatatatgt gaaatatata ccgatgttga tggcgtttac acaactgacc
     9901 cgagcgtatg ccacaaggca aggcgccttg acaaaatctc ttacgatgaa atgctcgaga
     9961 tggcaagcct tggggcaaag gttctgcaaa tacggtcagt tgagcttgcg aagaaatata
    10021 acgttccgat cctagttaag acttcttttg gagatggacc aggaacatta gtatgtaagg
    10081 agggtgcgac tatgtcaatg gaaaaagttg taattgcggg tgttacttat aataaaaatg
    10141 aagcgaagat tgttgttaga aaggtgcccg ataaaccagg tatggcagcg aagatattca
    10201 cagctctttc agatgcaaaa attgttgttg acgtaattgt gcagaatgtg agcaaaaagg
    10261 acaaaaccga tattaccttt accgtttcaa gaacggatgc caaaaaagca tataaaatta
    10321 tggaggaaat ggcaaagaag attggggcag ctggagtatc catcgacgaa gatatagcca
    10381 aggtatcgat agtgggttct ggtatgattt ctcatgcagg aattgcatct aaaatgtttg
    10441 cagttcttgc agatgaaggg attaatataa aggctattac cacatcggaa ataaagctct
    10501 cgtgcgttgt tgagagtaaa tacggtcaac ttgcagtcca gtcccttcat aaagcatttg
    10561 gtcttgacaa ggaagaaatt aaggaggaga tgtgacggtc gagttctacg acaccacact
    10621 gagggatggg gcacaatctg aagacattgc cttttctctt acagacaaac tgagaattac
    10681 tgagaagttg gatgagttcg ggatgcattt cattgaaggg ggctggcctg gttcaaaccc
    10741 aaaggatctt tcgtatttta aagatgtgaa aaggctgaac ctaaaaaact ctaagatcgt
    10801 tgccttcagc agtactatga aacctcactc aaagcctgaa gaggacgaga ttataaaagc
    10861 tatatttgac gctgatacag agtttgtggc cattgtcggc aagagttggg accttcatgt
    10921 aatcgatgct ttgaaggtaa gcctggacat gaacctcagg atgattgaca ggacaataac
    10981 ctttttaaag aaacatggaa agaaggtctt ttttgatgca gaacactttt tcgacggtta
    11041 caagaaaaac aacgcttatg cgatgaaggt tctggaggtg gctgttaact ccggcgctga
    11101 cgttgttgtg ctctgcgata caaatggcgg cagtatgccc ttcgaagtaa gtgatattgt
    11161 tgccaaggtt accaatatga ccgaggcaaa gctgggtatt catacccaca atgacacaga
    11221 aaacgctgtc gcaaattcgt tgatggctgt caaagctggg tgcaggcatg tacaggggac
    11281 aattaatgga tacggcgagc ggtgcggtaa tgcaaacctg tgttcaatca taccaaatgt
    11341 ggtactaaaa atggggcaca ggggaataaa accggaacgg cttatccatc tcagggaatt
    11401 gagccttttt gtagatgaga tggcaaatct ccttcccaat aaacaccagc catttgttgg
    11461 cgacagcgca tttgctcaca agggtgggat ccatgttagt gcaataagaa gaaacccaga
    11521 gacttatgag cacattaaac ccgagcttgt tggaaacgcg caaagggtgc tcatttcaga
    11581 tttatctggt gagagcagta ttttatacaa ggcaaaggag tttaacgttg acattgagaa
    11641 agataaaaaa cttgcccgtg aagtgatgaa aaatgtcaaa gacctcgaaa tgtatggcta
    11701 ccagtttgaa ggtgccgaag gctctctgga gctccttatt aagaaagccc ttggcatcca
    11761 taagaagtat ttcgatctta tcggattcag agtaatggtt gaaaaaaagg agaagaatca
    11821 ccctgtttca gaggcaacaa tccttgtcag agttgcggac acggtagaac atacggccgc
    11881 ccttggtgca ggcccggtta acgcccttga taatgctctc cgaaaggctc tccacaagtt
    11941 ctatcctaaa cttacagaga tgaatttagt ggattacaag gttagggtgt tgagcaccag
    12001 agacggaaca aaggcagcaa cgagggtact aattcagagc agtgacggaa agcaaacatg
    12061 gggaacggtt ggagtgtctg aaaatatcat tgaagcaagc tggcaggccc ttgttgatgg
    12121 aatagactac aaactgctca tcgaagagga gaaagaccgt tgaagaaact attgaatatc
    12181 agggatgcca caaaagaatt gaacattctt cgatccttac gaaattataa gcgacccttt
    12241 gaaatcgtcg gtgcgtatag attgattgat gatggttcaa gaccagaagc aactgttata
    12301 ataaaaagaa atgaccacga gacccatgaa gcatctacag gcgtaggtcc tgttgatgcg
    12361 ctggcaaacg tactcaagaa atctcttgca tctatctttc ctgtcatcca gcagattaaa
    12421 ctcgtagatt attcctcgag gatccacgag gcaaaatctg ggacagcagc acaggttgag
    12481 gtatcaataa tcttcacgga tgggcaggag atatggagcg ttgccgctat ttcggcaaat
    12541 attaatatgg gatcctttct tgccctactc gatgggtttg agtatgctat tctggcaagg
    12601 agcgaaaaga aacaaagctt atagctttaa gctgatggtg caggagaagt aaaaagttaa
    12661 tggtgacctt ccccatccca ttgactcata atttagcctt ccttttgatt tatcatttac
    12721 ccatcaatca gcaaaatgta gcttgataac aacattcatt atgctttata ttacggcatg
    12781 aggaggaatt ctggattata caataaaaat tggcggtgag gcaggccagg gcatccagac
    12841 aatcggcgac actttgtcca gggtattttc acgctgcggt tatcacgttt tcactcacca
    12901 ggatttcatg tcgcgtgtcc ggggtggtca caacttctat cagatgcgtg tctcggataa
    12961 agagatttct gcctcaagag gaccggtgga tattcttgtt gcactcgata gtgacagtat
    13021 ttcttcccat agtaaagagc tttctgccat aggtgttgtt gtttatgatt cagcagcgct
    13081 gaaagaaaaa catgatggat cacagttttt tgacgtccct ctccttgatc ttgcgttgaa
    13141 aaatggtggc gacaagatca tggtcaacac agtggctaca gggactgtac tgggaatgct
    13201 tggccttgga actgacattt ttctggatat tctccgggaa attctcggca aaaaagggga
    13261 agctgtgctt tctgcaaatg caaatgctgc ccgggcagga catgaatttg cagggcgtga
    13321 atgtttgcgg tgtgagtttt ctacgcctgc aattggcaca aaaaaaatgc ttattggcat
    13381 taatgaagca gtgggacttg gagctcttgc atctggttgc aagttttatt ctgcatatcc
    13441 catgacacca tcgacaggca tcatgctcta cattgcaagc aaagccaagg aatttgaagt
    13501 aattgtcgag caggctgaag atgagattgc agccatcaat atggcgctcg gagcttcttt
    13561 cgcaggtatt cgtgctatga ccggaacgtc agggggtggt tttgccctca tggtagaagg
    13621 cctttctctt gctgccatga cagaaacgcc cattgttatt gcccttgccc agagacccgg
    13681 acccgcaaca ggacttccga ctcgaaccga gcagggagat ctcctctttg ctctccatgc
    13741 cggccacgga gaatttccga gggtgatctt tgcgccagga acacctgaac aagcattcct
    13801 cctcacaaac aaagccttcg accttgcaca aaaataccag ataccggttt ttatcctcat
    13861 tgatcaatac ctttcagact ctcagtggac atatgatggg tttaacctca ataagcttac
    13921 cttgacagac tggcgggcaa ctgagtctga actggaaggg gttaaagatt acaagcgtca
    13981 ttttctaaca gatcatggca tatctccctt tgccaggcca ggcacttcga gacatctcgt
    14041 tgtgacggac agcgatgaac acgacgaaga tggacatctt attgaagatg ctgccacaag
    14101 ggttcagatg gttgataaga ggctttttag aaaaatgccc cttattcgat ccgaaattac
    14161 accccctgtt ttttatggca acagcaggcc tgacattatc ctcaccgggt ggggttcaac
    14221 atacgggatc atgaaagagg cagtcaatat cctttcagag acaacaaaag tcgcgatgct
    14281 ccatttcagc gagattttcc cttttccttc cagccaggaa ctcgattatc tggaaatact
    14341 gggaaaagca aacatgacaa ttattgttga acagaatgcc tcaagccagt ttgcacggct
    14401 tatgaaaacg gagacaggtt ttgagtttac cggaaagatc aatcgctacg atggcaggcc
    14461 cttcctcgtg gaagagcttt taggagagat aagtgcctac attggatgat tacaaaggac
    14521 aggagccagc ctggtgccct ggttgtggaa attttcccat cctcagggct ttcaaggagg
    14581 cgtttgtgga aatggacatt gaaccgcacc aatttctcat tgtctctggt ataggccagt
    14641 ccggtaagtt tccccattac ctgaagtgta acacctttaa cggccttcac gggcgtaccc
    14701 tcccggttgc aacaggggca aggcttgcaa accacgagat gcttgttgtt gcggtagcag
    14761 gagatggcga ctgttacggt gagggtggta accacctgat gcatgccatc aggcgcaaca
    14821 tcggtgttaa gctttttgtt cacgacaacc agatctatgg tcttacgaag ggccaggctt
    14881 cgcctaccag catggagggg atggttacta agaaccagcc ttttggtgta ttgtcagagc
    14941 agttaaaccc aatggccctg gctgttgccc tcgattgcag ctttgtggca cggggatatg
    15001 ccggtgacca cgagcattta aaggggttga ttaagacggc ggtaaatcac aaaggcttct
    15061 ccctcgttga catcttccag ccctgtgtta cctttaaccg ggtgaatacg tatgcatggt
    15121 atagccagcg cgtctaccac atagaggatg attacaatcc tgaagatcgt gtggcggcct
    15181 ttcagaaggc ccttgagtgg ggggacagga tccccatcgg cgttatttat cgcaacaatc
    15241 gacctacctt cgaagagagg gtgccagtaa taaacgagct gacacttgtc aaacaaccgg
    15301 tatcaataga gggcttaaaa tcaaaactcc gcaagacgat caaagagttt ttctaaaaaa
    15361 acagaatata aaacttgttt accatgagga cgaatacatg ctcgggcatt cgctgcaaac
    15421 atgcctgttc tgctcctaat cttatgaatt ttggaaaaca caaggtttga cctgcgcaac
    15481 tggggcctgg atttggagcg aggtgcaatg tttttattat
//
LOCUS       DAOOMC010000068        15415 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdus sp. isolate AMR_MDS_2685
            NODE_4081_length_15415_cov_5.671810, whole genome shotgun sequence.
ACCESSION   DAOOMC010000068 DAOOMC010000000
VERSION     DAOOMC010000068.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864760
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdus sp. (wastewater metagenome)
  ORGANISM  Syntrophorhabdus sp.
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae; Syntrophorhabdus.
REFERENCE   1  (bases 1 to 15415)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15415)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,729
            Genes (coding)                    :: 2,708
            CDSs (with protein)               :: 2,708
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 7 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15415
                     /organism="Syntrophorhabdus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4081_length_15415_cov_5.671810"
                     /isolate="AMR_MDS_2685"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2699756"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            609..1067
                     /locus_tag="PK125_08180"
     CDS             609..1067
                     /locus_tag="PK125_08180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB38124.1"
                     /translation="MKKILFVVVLILVLFLTLSSLSTAADWVYYSSDNLDNASFYDRE
                     SISFPSKGVVRVWIKQLYSEKGKMNEITERTKSNLTIDGLNELSYALCLHEIKCDAKE
                     DAIVTCTSYSNSGNTLNSFNISKDRLKWEPIYPDSISEALWKAVCKREGK"
     gene            complement(1191..1625)
                     /locus_tag="PK125_08185"
     CDS             complement(1191..1625)
                     /locus_tag="PK125_08185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB38125.1"
                     /translation="MTHEDTGNYAAKRRGAALNETIAARIKEKISESRISCAEAHSIA
                     AELNVPPEDVGTAIDLLEVRIIKCQLGLFGYGKEKNIPALPDKINPDIEQTIKSSLVD
                     NRLPCSTAWGIAKKFKVSKPVISAVCEAMKIKIAPCQLGAFK"
     gene            complement(1719..2444)
                     /locus_tag="PK125_08190"
     CDS             complement(1719..2444)
                     /locus_tag="PK125_08190"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HPB38126.1"
                     /translation="MTKVNEGVYFIQGQDEFIPDSHVYVIGDPASNDLSLIDVGLTGK
                     GNYKVQSIKGLGIELEAVKRVIMTHTHLDHIGCLSEIRKAIPSVELWVHKLEAELLEK
                     GDDRGVYGMNEFKSMCQMQYGLKSDAFKLKVDKKLEGGESLHIGGMKWEVIHIPGHSM
                     GGIALYHSSLKILIPGDVVYADYAIGRFDLYGANADALKKSLYHLADLEIDMLLPGHN
                     QIVKSLPSDYIRKTAKMWEPYLV"
     gene            2667..2879
                     /locus_tag="PK125_08195"
     CDS             2667..2879
                     /locus_tag="PK125_08195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB38127.1"
                     /translation="MTVSTNQVSAVIRTYLKNTKDKIEKVSNPALNPEGVHDEVTVSD
                     DAKKILFERIGKRVLEKLKNELARNY"
     gene            complement(2993..3172)
                     /locus_tag="PK125_08200"
     CDS             complement(2993..3172)
                     /locus_tag="PK125_08200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB38128.1"
                     /translation="MVKPIKKVIGGESTYNDDDELVKKQRKLLVESLVKDFYPSKFSA
                     RHIIEKRDMERTKRS"
     gene            complement(3352..4350)
                     /locus_tag="PK125_08205"
     CDS             complement(3352..4350)
                     /locus_tag="PK125_08205"
                     /inference="COORDINATES: protein motif:HMM:NF024876.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase MA family metallohydrolase"
                     /protein_id="HPB38129.1"
                     /translation="MRSVSGQGMGRHGIQRYGINLGMIRKVCFRFLIATLVVLFSFTH
                     IYSDTGAVISDNTVIIQFDGPLKNAAREVIELYPRVKKELEGILGWEIDFKPSILLIK
                     ENSKFQEVVENPHIIAFADPHRNLIAIDYSRMNVSPFTLEVTVKHELCHLLLGKHIPD
                     DRLPRWLNEGFCQWVTGGIGEFMVDDRQPNLRKASLSGQLIPLWLLSGRFPSQRDQMA
                     LAYEESRSVVEYMIAEHGQSGVVNIMNNMKHGDTVDEAIKKVLFVSIGELERQWILYL
                     RERVTWAGYLAANIYLIILFAAALLTICGFLRIVMRRRLRAAQVEEDNEDDEVTGG"
     gene            4541..4993
                     /locus_tag="PK125_08210"
     CDS             4541..4993
                     /locus_tag="PK125_08210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB38130.1"
                     /translation="MKIEFTQEQYEALLKIVYLGNWMASSTKETPEKEYENIEQYILS
                     FAKDFNLEKFVGFDQKQKSYYPSEEFENSTDVVQLIDAYDEYQVWEALVLSLSHRDIV
                     KKYGEKKVEEMSDEELLEKEYPLIQKYEDEFREHGFDNLYVRSKKSSG"
     gene            5031..6827
                     /gene="lepA"
                     /locus_tag="PK125_08215"
     CDS             5031..6827
                     /gene="lepA"
                     /locus_tag="PK125_08215"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016291381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HPB38131.1"
                     /translation="MKQIRNFSIIAHIDHGKSTLADRLIQFAHLVDDRQFRDQILDNM
                     DIERERGITIKSQSVNLPYTSRSGEEFELNLIDTPGHVDFSYEVSRALASCEGVLLLV
                     DATQGVEAQTLANLYAAMEHNLVIIPVINKIDLPSANIDGVKEEIEHELGLDPESTLL
                     CSAKEGRGIQEILEAIVARVPPPKGDIDKPLAALIFDAQYDSFRGTIVSCRIFDGTLK
                     PGSVIRPMSTGATYKVEEVGIFRLKPEPVKELSAGMVGYIIAGIKTVGDTRIGDTITL
                     DANPVSEPLPGFREVKPVVFSSIYPIASDDYQSLAESLEKYKLNDAALIYQKDSSAAL
                     GQGFRCGFLGLLHLEIVQERLEREFDQSIILTVPSVQYRFGLADGTTVTVDNPAHYPD
                     PTYIRTTEEPYIRANILVPERYVGAVMKLCLDRRGVNQRMNYPTPGRVEITFDMPLAE
                     VVFDFYDKLKSITQGYGSFDYELIDYRESDLVKLDILVNGEKVDALSIIVHKERARDR
                     AVIVCDRLREEIPRQQFKIAIQGAIGGKIIARSTITPFRKDVTAKCYGGDISRKRKLL
                     EKQKKGKKRMSVVGNVEIPQSAFLAALKTDNE"
     gene            6904..8064
                     /gene="hemW"
                     /locus_tag="PK125_08220"
     CDS             6904..8064
                     /gene="hemW"
                     /locus_tag="PK125_08220"
                     /inference="COORDINATES: protein motif:HMM:TIGR00539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HPB38132.1"
                     /translation="MNIKDRAKRGNRMNPQSDNMPGLYIHIPFCKTKCPYCDFYSVTN
                     VDLAEEWLTAILKEMSHYSNIFGTFDTLYLGGGTPTVLSNTVLVSLFRELRRHFNFHD
                     DTEITVEANPNDITLEKLALFKLLGVNRLSIGAQSFNDEELLLLKRRHSVKGIEEALY
                     CVNQAGFLNFGIDLIYGIPGQPMASWITSLQKALGFKPTHLSCYQLTIEQTTPFGKML
                     QEGNLQGIGEKQGKDFFLTTSRFLENNGFMHYEISNFSRKKRFRSRHNLKYWKHVPYL
                     GLGPGAHSYLNGKRWWNVKSVKDYCYQLSQGLKPIQASESLSPEQLRLEKLFLGLRTN
                     EGIKIDVAFPDYQTSASLENMVKNRLITVRGDRLIPTKKGFLMADRLPLLFP"
     gene            8228..9283
                     /locus_tag="PK125_08225"
     CDS             8228..9283
                     /locus_tag="PK125_08225"
                     /inference="COORDINATES: protein motif:HMM:NF017847.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF933 domain-containing protein"
                     /protein_id="HPB38133.1"
                     /translation="MYISIIGKAGSGKTTLFQALSGVTKSLNGGSRDIVAIDVPDERL
                     EYLSSVFSPRKTVYARIDVSDTIAIDKGDLKNETLNQKSVQQLRSSDAFLLILKNFDN
                     GEEINPVDDFLAIYNEFILADMVQIENRIERIERQYGKKDAPPYQQERGLLEKCLDHL
                     NNGAPLSLLALPEEEEKRIRGFQLLSRRPMMVVVNCTEQTLSSAEFVAEEFRNTLPPH
                     IPVLAVCAKLEAELALMSPEERTIFMREYGIREAIRSRIIHIALDTLGLISFITVGDD
                     ECRAWPIKKGMNAQDAAGTIHTDLANKFIRAEAVSYKAFVEHGGFAGCKKSGLWRLEG
                     KTYVVQDGDILSIRAGN"
     gene            complement(9373..11355)
                     /locus_tag="PK125_08230"
     CDS             complement(9373..11355)
                     /locus_tag="PK125_08230"
                     /inference="COORDINATES: protein motif:HMM:TIGR02123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter fused permease subunit"
                     /protein_id="HPB38134.1"
                     /translation="MEEQEKSVLNQSQESLTEEQKKKLQELIEEEEGVTRKVGGFWNA
                     IISILAVAMSLFAIYSAIQPVTTQILRGVHVAFLLAISFLYYPIAKKYKGKINVFDIF
                     LCLLGMACIIYMLVDFEDFIYRAVTPELWDMIFGVLFILLVLETTRRTSGLIMPVTCI
                     IFLIYAYVGPWLPAPWTHRGYDLERIVGHMYMTLEGIFGVPIDVSSSIIIMFCIFGAF
                     LTVSGAGKFYVDFAFTAMGKKPTASGRAVIATSYLLAMASGSGVANTVAIGTVAYPML
                     QKSGYDKENAGGFLAAGGMGAITTPPVMGAAAFLIAEFLRISYVDVILLAIIPAALYY
                     WGLLLMVEFDAKKFGLKAVQIEKKYTLWQLTKLYGYHFLPLIAIVAFLVKGFTPQVSV
                     FAAILTCFLSSFIRKESRFTVPKLIEALKQGSLTVLNVAAICASAGIIVGTVSLTGLG
                     LKLSSIIIGYAGGSLIITTLFTGILLWIIGLAVPITATYIIAAVIAAPALTQLGVPEY
                     AAHMFIFYYALLSEVSPPTALSPFAAAAITGGDPFKTTMMSWKYAITAFLMPFIFTVL
                     PAGRAILLHWEGIGLASGLWTIAAAFIGIGMLASGCSGWLLKKSSVLERIVIIIGALA
                     FVYPSRVGDLLGFACLILVIVMQRLRKGGGTPVVAT"
     gene            complement(11492..12502)
                     /locus_tag="PK125_08235"
     CDS             complement(11492..12502)
                     /locus_tag="PK125_08235"
                     /inference="COORDINATES: protein motif:HMM:TIGR02122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TAXI family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HPB38135.1"
                     /translation="MDFESLIKDREVKRRDFLKLTGVLGGTILLGNVGKVFAQTKKRI
                     SVATGGMGGVYFVIGGGIASILTKYAGVEAAAEVTAASVDNCKLLAAKKSDMGLVIAD
                     TGYDSIRGTGIFKGKALPLKTLCTIYPHYNQVVTLEGKGIKKVADLKGKRVSTGAPGS
                     GTEVVAFRILEAAGINHEKDIKKDRLSAAESAGALKDGKIDAYFWSGGVPTASVLDLA
                     ASPGVKISILAHDDLVEKMNAKYGPIYYKTVIPKKSYTGLENDVPVSGVTNLLMCHQD
                     MEEQLAYDVVKTIFEHISDLAAIHAEGKNINIKDNALNKFVPYHKGAAKYYKEKGITV
                     LT"
     gene            13056..14033
                     /locus_tag="PK125_08240"
     CDS             13056..14033
                     /locus_tag="PK125_08240"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF013323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HPB38136.1"
                     /translation="MGMYPEIDDKLLSLEEAVGRFIKNGSQIAIGGFTVTRNPMALAY
                     EIVRQGIKDIHLVCHSHGQALDILIGAGCVSRLEIAYGGNGRYAPTCIRFKKAIQRGE
                     IQFEDYSNYQMSLRFFAGSLGIPFIPTKSGLGSDLIDIEGFHPEIRKEKKVASKKYVV
                     TQNPFNGEDDKVLLLPALNPDVAIMHAQYVGEDGTVRIKGLTFADMEQAKSADVVIVT
                     CEEIVPRSFIRLDPDQNSLPPFFVDAIVKVPYGAHPTAVYTYYDYDPKHLNLYKKVAE
                     DDKAFKVYLDEWVYGVRSHQEYLEKVGNTALFLIKANPILGYAVGLDRK"
     gene            14046..14804
                     /locus_tag="PK125_08245"
     CDS             14046..14804
                     /locus_tag="PK125_08245"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF013323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HPB38137.1"
                     /translation="MAAYTDNEMMALAAGRFIRNGDIVFAGTGVSMLAATAAKRIYAP
                     KAVVFFETGGIDPSLEEIPMAVADPRIMYGTCLNSGLIEAFSIVGHRKLHTIAFLGAA
                     QVDKYGNLNTTVIGDYHKPKTRFSGSGGACDVASLASGVITFMQHQKRRFVEKLDYLT
                     SVGWYKGGDSRRRLGLPRGGALAVVTNLGVMKFHETTREMYLAEYYPGITVDKIVENT
                     GFLVDTSRAVEAAPPTPEELRILREEVDPQKLIL"
     gene            14840..15406
                     /locus_tag="PK125_08250"
     CDS             14840..15406
                     /locus_tag="PK125_08250"
                     /inference="COORDINATES: protein motif:HMM:NF039354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6125 family protein"
                     /protein_id="HPB38138.1"
                     /translation="MDIPDNPVHEELEKLPKETLIELIKMYSRNWHTCDGLWFSGVEE
                     QYGTEKALEIDINMWSISSRLEAKRIKEVLAIPDNAGLDEVLRAINFMSWAAKCSYRI
                     DKNGDSALLTVISCPPQEARIKAGKGVFACRPTFELGFGNVARVIDPSVKVTCKYCPP
                     GPYPADSWCQWEFNRNHAIPLALCDITR"
BASE COUNT         4407 a         3488 c         3321 g         4199 t
ORIGIN      
        1 tgagatgtat tcctttatag aattaattgt attctattag ggaatgatag tattcgttag
       61 tgcataaaca tattcctaaa tggaatgtct gttataactt atatcaatta tttcttgacc
      121 ttgtacaaaa atatctgaat atagattctt tacacaaact cactcatatc gtttcacatt
      181 atgaaactat gtactcacca ccattatcat caaaattact cttcttctat agggaagctg
      241 tatggttttc acgtttatta taagtatgtt atgaatcaga aacagcagca tcatcatcat
      301 cagcatccta ctgtgcaggt gtatggaata tcatattctt tttataagat aacgtgctct
      361 tgtgtgagat ggtgcactct tgtgtgagat agtatatccc attgtaaatg cagtatcatt
      421 tatcatagaa gcgcatccct cccaatatat tgacatcagc atcgagacat ttcaccgtga
      481 acactgggaa agaaaagtct atggggggag tttcggtttt catcgataac ggatagaagc
      541 tcggattttt gtagcaaaat tcaagttgtg ctatacttat aagtagatag cgaagggggg
      601 agggtcagat gaaaaagata ttgtttgttg ttgttttaat attggtgtta tttcttaccc
      661 tgtcaagctt atcaactgct gcggattggg tgtattatag ttcagataat cttgacaacg
      721 ccagtttcta tgaccgtgag tcgattagtt ttccatccaa gggtgttgtt cgtgtctgga
      781 taaagcaact ttattcagag aaagggaaaa tgaatgagat tacagagcgg actaaatcga
      841 atttaacaat tgatggtctc aatgagttat cgtatgcttt gtgcctacac gaaatcaaat
      901 gtgatgcaaa agaggatgct attgtgacat gtacatctta ttccaactct ggtaataccc
      961 ttaatagttt caatatctca aaagaccgtc ttaagtggga acctatttat cctgattcca
     1021 tttcagaagc tctgtggaaa gctgtgtgca aacgggaggg taaataagga agtatagcat
     1081 tcaattaatc tcttcctttt tactctcttt ccccaataat atcaatctct gctatttatt
     1141 cgagccccgt cagccacccc actgtgcaat tcaaatcaat cccgggaaag ttatttgaat
     1201 gctccgagct ggcacggggc aattttgatc ttcattgctt cacatactgc cgagatcact
     1261 ggttttgata ccttaaactt ctttgcaatt ccccatgcag tggagcatgg aagccgatta
     1321 tcaactagag atgacttgat ggtttgctca atgtcaggat ttatcttgtc cggcaatgca
     1381 ggaatgttct tctctttccc ataaccaaaa agcccgagct ggcacttaat aatccgaact
     1441 tcaagaagat cgattgccgt tccaacatct tcgggcggca cattcagctc tgctgctatg
     1501 ctgtgtgctt cagcacagga tatcctgctt tcagatattt tttcttttat tcgtgctgct
     1561 attgtttcgt tcaatgcggc accccgtctc ttcgctgcat aattccctgt atcttcgtgt
     1621 gtcatcgatt gttctcctca atattattat aggtttggtt atgtaatccc taccttttca
     1681 cttctttttt tttgactctt cactgaccag cctgccaatc aaaccaagta tggctcccac
     1741 atcttcgcag tcttcctgat gtaatcagaa gggagacttt taacaatctg gttgtgacca
     1801 ggcaatagca tatctatttc aagatcagcc agatggtaga gtgacttctt cagcgcatct
     1861 gcattggctc cataaagatc aaatcgccct attgcataat cagcataaac aacatcccca
     1921 ggaatgagaa tctttaggga tgaatgatac agagcaatgc cacccataga gtgtcctggt
     1981 atatggatta cttcccattt cattcctcca atatgaaggc tttctccacc ttccagtttt
     2041 ttgtcaacct tgagcttaaa ggcatcagac ttcagtccat actgcatctg acacatgctc
     2101 ttaaactcat tcatgccgta tacaccccgg tcgtctccct tttcaagcaa ctctgcttcc
     2161 agtttgtgga cccagagttc aaccgatggt attgcttttc gtatttcgga caagcaccct
     2221 atatgatcaa ggtgggtatg ggtcatgata acgcgcttca ccgcttccag ttctattccc
     2281 aaccccttta tggactgtac cttataattg ccttttccgg taagtcccac gtcaatcaat
     2341 gagagatcat tggatgccgg gtcgccaata acatatacgt gggaatccgg aataaactca
     2401 tcttgccctt gaataaaata gactccttca ttcacttttg tcatgcgtta cccctttcaa
     2461 tgtaaatcgt atatcatgct tcgaatattc tatcatcttc tgttttcatt tttgttccag
     2521 gttgtattgt acgttgaggc gttatggcag gtcaaggcaa ttattatgca taccggaata
     2581 aagcccgaca ctgcggaaca gcctggaaaa aatccttaag gaaatgatgt cgggattcga
     2641 taatggacta aagggcaatc tattagatga cagtatcaac aaaccaggta agtgcagtta
     2701 taaggactta tctgaaaaat acaaaagata agattgagaa agtctccaac cccgccttaa
     2761 accctgaggg cgttcatgac gaggtaactg tatctgatga tgccaagaag attctatttg
     2821 aacgaattgg caaacgtgtt cttgagaagc tgaaaaacga actggctcga aactattagg
     2881 cttcttcaga tacctttctt aatcatctta cacaaactaa tcatgttcaa ccaacagctc
     2941 gtggtctgcc atattgtggc atttattgcc aatattttga ctttatcctg tattaagatc
     3001 tttttgttct ttccatatct cttttctcaa ttatgtgacg cgcactgaat ttggaaggat
     3061 agaaatcttt caccaatgat tccacgagca gttttcgctg tttctttacc agctcatcgt
     3121 catcattata agtgctctct ccacctatta ccttcttaat cggcttcacc ataattcccc
     3181 acctcctttt ttgagtatta tacattagta agcaaaaagt atgcctcaat atggtggatt
     3241 gtgtatcatc gaaagattgt ataatgtatt ccccatgctg tatttcatgg taaaaccgtt
     3301 attgttaaca gagaattcgg ataaacatta ccttttattg tgtcaccgcg ctcagccacc
     3361 agtcacttca tcatcttcat tatcttcttc cacttgtgca gcccgaaggc gtcttctcat
     3421 cactatccgg agaaacccac agatagttag caatgccgcg gcaaaaagaa taatgaggta
     3481 aatgttggct gccaaatagc cagcccacgt gactctttcc cggagatata gtatccactg
     3541 cctttcaagc tcaccgatgg agacaaataa aacctttttg attgcttcat caacagtgtc
     3601 gccgtgtttc atgttattca tgatgtttac aactccactc tgaccgtgtt ccgcaatcat
     3661 atactccacc acgctcctgc tttcttcgta ggcaagggcc atttggtccc tttgggaggg
     3721 aaatcttccg ctcagcaacc acaaagggat tagttgcccg gatagagaag cctttctcag
     3781 gtttggttgc ctgtcatcaa ccatgaactc cccaatcccc ccggtaaccc actgacagaa
     3841 tccttcattc aaccaccggg gtaatctgtc atctggtata tgttttccaa gaagaaggtg
     3901 gcacaactca tgttttactg tcacctcgag ggtaaagggg ctgacattca tccgagagta
     3961 gtcgattgca ataaggttcc tgtggggatc tgcaaaggca attatatggg gattttccac
     4021 cacctcctga aactttgaat tctcttttat caaaagaatg gatggtttaa aatcgatctc
     4081 ccatccaagt atgccttcta gctctttttt aacacggggg taaagttcga taacctctct
     4141 ggcagcattt ttcagtggcc catcaaattg aataataaca gtattgtccg agataacagc
     4201 ccctgtatcg gaataaatat gcgtgaagct gaacagaaca accagggttg caatcaaaaa
     4261 cctaaagcat actttacgta tcattcctag atttatacca tatctctgga tgccatgcct
     4321 ccccataccc tgccctgata ctgacctcac cttgcccttc cggtcccgcc agggcaggag
     4381 atggtagttc actttgaaga aggaccaaaa aacagttgaa gtgaacgaaa gtcactggta
     4441 gactaattga ctttcaaaga agacataatt taatattcca gagtaaaaga gaagcaaaaa
     4501 aatgctccct gtgtaagaaa aaaattttta ggaggaaatt atgaagattg agttcaccca
     4561 agaacagtac gaagctttac tcaaaattgt gtatctcggg aactggatgg caagctcaac
     4621 aaaggaaact ccggaaaagg aatatgaaaa cattgagcag tacatactct cctttgcaaa
     4681 agatttcaac ttggaaaaat ttgtaggctt tgaccagaaa cagaagtcat attacccatc
     4741 agaagagttc gaaaattcaa cggatgtggt tcagcttata gatgcttacg atgagtatca
     4801 ggtgtgggag gcccttgtgc tgtcactctc tcatcgtgac attgtaaaga agtacgggga
     4861 aaagaaagtg gaagaaatgt ccgatgaaga gctcctcgaa aaagagtatc ctttgatcca
     4921 aaaatatgaa gatgaattcc gggaacatgg ttttgataac ctttacgtga gatcgaaaaa
     4981 atcgtcagga taatgatttt tgttctcact aaaaagaagt tgatcatttt atgaaacaaa
     5041 tccgtaattt cagtatcatt gctcatattg accacggcaa atcgaccctc gcggaccgtc
     5101 tgattcagtt tgcccatctt gttgatgatc gtcaattcag ggatcagatt cttgacaaca
     5161 tggatattga acgtgaacgg ggtatcacta taaaaagcca atcggtgaac cttccctata
     5221 caagcagatc cggcgaggag ttcgagctaa acctcatcga cacaccaggg catgttgatt
     5281 tttcctacga ggtctcccgc gctcttgctt cctgcgaagg ggtgttgctt cttgttgatg
     5341 ccacacaggg tgtggaagcc caaacccttg ccaatcttta cgcagccatg gagcataatc
     5401 ttgttataat ccctgtaatc aacaagatag acctgccctc agccaacatc gatggggtca
     5461 aagaagaaat agaacatgag ctcggtcttg atcctgagtc tacactgtta tgctcggcaa
     5521 aggaaggaag gggtattcaa gaaattctgg aggccattgt tgcccgtgtg ccacccccga
     5581 aaggtgatat tgataagcct ctggctgccc tcatatttga cgcccagtat gattccttcc
     5641 gcggaaccat agtaagctgc aggatcttcg atggcacttt aaaaccaggg agtgtgatcc
     5701 gccccatgtc aacaggggca acctacaaag tcgaggaggt cgggattttc aggcttaaac
     5761 ccgaaccggt caaggagctc tctgctggca tggtagggta catcattgcc gggattaaaa
     5821 cagtaggtga cacaagaatc ggtgacacca taacccttga tgcaaaccct gtatccgagc
     5881 cacttcccgg gttcagagag gtaaaacccg tagttttttc ctccatctat cctatcgcat
     5941 ccgacgacta ccaatccctt gctgagtcac tggagaaata taagctcaac gatgctgcgc
     6001 tcatttacca gaaagactca tctgccgcat tgggtcaagg tttccgttgc ggctttctgg
     6061 gccttctaca cctggaaata gttcaggaac gcctggaaag ggagtttgat caatccatta
     6121 ttctcacggt acccagcgtg caataccggt ttgggcttgc tgatggcaca acagtcactg
     6181 ttgacaaccc tgcccattac ccggatccaa cctacatcag aacaaccgag gagccttaca
     6241 ttcgggcaaa cattctggtg cctgaacgtt atgtaggtgc ggtgatgaag ctctgcctgg
     6301 accggcgagg ggtaaaccag cgcatgaact acccaacccc tgggagggta gagattacct
     6361 ttgacatgcc ccttgcagag gttgtgtttg acttctacga caaactgaaa agtattactc
     6421 aaggatatgg ctcctttgac tacgagctca tcgattaccg ggaaagtgac ctggtaaagc
     6481 tcgatatact cgtgaatgga gaaaaagtgg acgctctttc catcattgta cacaaggagc
     6541 gtgcccgcga cagggcggtg atagtctgcg acagactcag agaagaaata cctcgtcaac
     6601 agtttaaaat agcaatacag ggagctattg gcggaaagat aatagccagg tccaccataa
     6661 cacctttcag gaaagacgtc acagcaaagt gctatggcgg tgacatttcc agaaaacgga
     6721 aactcctcga aaaacagaaa aaaggaaaga agaggatgag tgttgtcggt aatgtcgaaa
     6781 taccccagag tgcctttctt gcagcactga agacggacaa cgaataaaac ccacgatgag
     6841 taatcggtaa tgcgtaatgg gttttaagcc gattactgtt tacgcgtcac acgtcatgaa
     6901 aatatgaata taaaagatag agctaaacga ggcaacagga tgaacccgca atcggataac
     6961 atgcccggcc tttacatcca cattccattc tgtaagacaa aatgcccgta ctgtgacttt
     7021 tattcagtga cgaatgtcga cctggcagaa gaatggctta cggcaatcct gaaggagatg
     7081 agccattata gcaacatatt tggtaccttt gatactttat atttaggtgg cggaacaccg
     7141 acggtgctga gtaacactgt gttggtatca cttttccggg aactccgcag gcatttcaat
     7201 ttccatgatg atactgaaat aaccgtagaa gctaatccga acgacattac actggaaaaa
     7261 ctcgcattgt tcaaactgtt gggcgtaaat cgtttaagta ttggagccca atcgttcaat
     7321 gatgaagaat tactattatt aaaaaggagg cattcggtca agggtataga agaagccctt
     7381 tattgcgtca accaggcagg gtttttaaac tttggcattg atctcatata tggcatacca
     7441 ggtcagccaa tggcaagttg gattacaagt cttcagaaag cacttggatt taagcccacc
     7501 catctgtcct gttatcagct gactattgaa cagacaaccc cgttcggcaa aatgttacag
     7561 gagggaaatc tgcaaggtat tggtgaaaaa caggggaagg atttctttct tacaacatca
     7621 agatttcttg aaaacaatgg ttttatgcac tacgaaatct caaatttctc cagaaagaaa
     7681 cgattccggt cacgccataa cctcaaatac tggaagcatg taccttacct cggccttggt
     7741 ccgggcgctc actcctacct gaacggaaag cggtggtgga acgttaaatc ggtaaaagac
     7801 tactgttatc aactcagcca gggtttgaag cccattcaag cttcagagtc cctttcccct
     7861 gagcagctac gccttgaaaa actttttctt ggcttgcgga ccaatgaggg cataaaaata
     7921 gatgttgcat ttcctgatta tcaaaccagt gcctctttgg aaaacatggt gaaaaaccgc
     7981 ttaataactg tccgtggtga caggctcata cccacaaaaa aaggtttcct catggccgac
     8041 cgtcttcccc ttctcttccc ataacttttc atttttttgc cacctctgac tccctattgc
     8101 catagtctgc cacctgaatt ttcactgcct ttctatcttt ctgccaaacc ctcgttgaac
     8161 ttgcatttac aatgaaattg tcgtacaata cccgatcgtt tcaaatcctc gtgattacgg
     8221 agcaaagatg tacatttcaa taatcggcaa ggccggttcg ggtaaaacta cactatttca
     8281 ggcattaagc ggtgttacaa aatcgttaaa tggcggatct cgcgatatag ttgcaattga
     8341 cgttcctgat gagagactcg agtatttgtc aagcgttttc agcccaagaa aaactgtata
     8401 tgctcgcatc gatgtatctg acactattgc cattgataaa ggtgatctga aaaacgagac
     8461 tctcaatcaa aaatccgtac aacagttaag atcaagtgat gcttttttgc tcattcttaa
     8521 aaattttgat aatggggaag aaatcaatcc ggtggatgac ttcctggcta tttataatga
     8581 attcattctt gctgatatgg tacagattga aaatcgtata gagcggatag aaaggcaata
     8641 cgggaaaaaa gatgctccac cataccagca agaacgggga ttgctggaaa aatgccttga
     8701 tcatctgaac aatggagccc ctctgtctct tctggccctc cctgaagaag aggaaaaaag
     8761 gataaggggt tttcagcttc tttcccgaag accaatgatg gttgttgtga attgcactga
     8821 gcaaacatta tcgtcagcgg aatttgttgc agaagaattt cgaaatacat tgccgcccca
     8881 tattcctgtc ctggccgtat gtgctaaact ggaggcagaa ttggctctta tgtctcctga
     8941 ggaacgcact atcttcatga gagaatacgg gatcagggag gccattcgca gccgcatcat
     9001 tcatattgcc cttgacactc tgggcctaat atcgtttatc accgttggag atgacgaatg
     9061 ccgtgcgtgg cccataaaaa aaggcatgaa cgcccaggat gctgcaggta cgattcacac
     9121 tgatctcgcc aataaattca ttcgcgctga agcagtgtcg tataaagcct ttgtagaaca
     9181 tggcgggttt gccggctgca agaaatcagg cctctggcga ctcgagggaa aaacatatgt
     9241 ggttcaggat ggggacatcc tctccataag ggctggaaac tgattcattt catcctgtat
     9301 tcaggataat tccaatgttg aacatgcaaa ggcaagatga aaagttaggc cttttacggt
     9361 tcgactcaat tgttatgtag caacaacagg tgtccccccg cctttcctca gcctctgcat
     9421 aacaataact aagataagac aggcaaaacc cagaagatca ccaaccctcg aaggataaac
     9481 aaaggcaagg gccccgatga ttatcacaat ccgttcaagg acacttgatt tcttcaacaa
     9541 ccagcctgaa caccctgatg caagcatacc aatcccgatg aaggctgctg caatcgtcca
     9601 caacccagag gcaaggccaa ttccctccca gtgcaacaga atagctcttc ctgcaggtaa
     9661 gacggtaaat atgaacggca tgagaaaggc tgtgattgca tacttccatg acatcatggt
     9721 cgttttgaag ggatcgccac ctgttattgc tgcagcagca aaaggagaga gggctgtggg
     9781 cggagaaacc tccgatagta aagcatagta gaagatgaac atgtgagcag catattcggg
     9841 caccccgagt tgagttaacg caggtgctgc tatgacagcc gcaatgatat aggtagctgt
     9901 aataggcaca gccaatccga taatccataa tagtattcca gtaaaaaggg tggttataat
     9961 gaggctcccc cctgcatagc caattataat tgacgacagt ttcagcccca gacctgtgag
    10021 actaacagtg ccgacaatta tacctgcaga ggcacagatg gctgccacat tgagcaccgt
    10081 taatgatcct tgtttcagcg cctcaataag ctttggtaca gtgaacctag attctttccg
    10141 gataaaggat gataagaagc aggtcaggat tgctgcaaaa actgaaacct ggggagtaaa
    10201 tcctttcacc agaaaggcta cgatggcgat aaggggtaga aaatgatatc catacagttt
    10261 tgtcaattgc cacagggtat attttttctc aatttgaaca gccttcaagc caaacttttt
    10321 cgcatcaaat tccaccatga gaagcagtcc ccagtaataa agagctgccg gtataatagc
    10381 taacagtatg acatcgacgt agctgattcg taaaaattct gctatgagga atgctgctgc
    10441 acccatgacc ggtggcgtgg taatagcccc cattccacct gcagccaaaa aacctcctgc
    10501 attctctttg tcatatcctg atttctgcag catcgggtat gctaccgttc cgatggcaac
    10561 agtgtttgct accccagacc cggaagccat ggccagaaga taggaggtag ctattactgc
    10621 acgacccgaa gctgttggtt tcttccccat ggcagtaaaa gcaaaatcta cataaaactt
    10681 gccagccccg gacacagtga ggaaggctcc gaatatacag aacatgataa tgatagagga
    10741 tgatacatct ataggcacgc caaagattcc ttccaacgtc atatacatgt gacccactat
    10801 acgctcaagg tcataacccc tgtgtgtcca gggtgcaggg agccacggac caacataggc
    10861 atatataaga aagatgatac atgttacggg cataatgagg ccagacgttc tgcgagtagt
    10921 ttcaaggacg agaaggatga agagaacacc gaaaatcatg tcccacaatt caggtgtgac
    10981 tgcccgatag ataaaatctt cgaaatctac gagcatataa ataatacagg ccatcccaag
    11041 caggcagaga aaaatatcga aaacgtttat ctttcccttg tattttttag ctatagggta
    11101 atagagaaaa gagattgcca gcagaaaagc aacatggaca cctcgcaaga tctgagtagt
    11161 cacgggctgt attgccgaat aaattgcaaa caaggacatt gcaacagcaa ggatgctgat
    11221 aatggcattc caaaagccac cgacctttct ggtcacgccc tcttcttctt cgatcaattc
    11281 ttgtaatttc ttcttctgtt cttctgtcaa gctttcctgg gactgattaa ggacggattt
    11341 ttcttgctct tccattgaat acccccaaga gataagaaat atgcatttga taacctgctg
    11401 ggctggcttc tgtcgcgcta gggcctaaat atctaagcag ccagcctaac gaaggcagag
    11461 gggggaaccc ccactgcctt agaattttca tttacgttaa aactgttata cctttttctt
    11521 tgtagtattt tgctgcacct ttgtgatacg gcacaaactt attcaaggca ttatccttga
    11581 tgttgatatt tttcccttca gcatgaattg cagccagatc gctgatgtgc tcaaaaatag
    11641 tctttacaac atcatatgcg agttgttcct ccatatcctg gtggcacata aggaggtttg
    11701 tgacaccgga tactggaaca tcgttttcaa gacctgtata tgatttcttt ggaatcactg
    11761 tcttatagta aatgggtccg tatttggcat tcattttctc caccagatca tcgtgggcca
    11821 gaatagaaat tttaacaccc ggagaggctg ccaaatctag gactgatgct gttggaacac
    11881 cccctgacca gaaataggca tctattttac catcttttag agcccctgct gattcagcgg
    11941 cgctaagcct gtccttcttg atgtcctttt catggttaat gcctgctgct tcaaggattc
    12001 taaaagcaac aacctctgta ccgctgcccg gggcccctgt ggaaaccctt tttcctttga
    12061 ggtcagcaac cttcttgatc cctttgccct ctaaggttac aacctgattg tagtgtggat
    12121 atatagtgca caacgtcttc aatggaaggg ctttcccctt gaagattcca gtgcccctta
    12181 tggaatcata gcctgtgtct gctataacaa gacccatatc tgactttttg gcagccagaa
    12241 gtttacagtt gtccaccgag gctgctgtaa cctctgcagc tgcctcaact cctgcgtatt
    12301 tggtcagaat gctcgctatg ccccctccaa tgacaaagta gactcccccc atgcctcccg
    12361 ttgctacaga tatccttttt tttgtctggg caaacacctt gcccacgttg ccaagcagta
    12421 ttgttccgcc aagtacaccg gtcaatttga gaaaatctct tctttttact tctctgtctt
    12481 tgattaagct ttcaaaatcc ataataatac ctcccttcat cgttatatcc ttgcgccata
    12541 cctttgcaat ggcgcaataa cagatacgga tattcattct aaatttttgg tggcacgtat
    12601 atttgaattc tatggtatta cacacaacaa tgtcaacttt tttattattt tatataatgg
    12661 ccaattaccc taatttccgg taatagagcg tgtacagaga cgaaatagag cgacaatgag
    12721 ataattcttt tgtatacaac atcatgcaat cgactcgcac ataccgtcag ttcagcagaa
    12781 atatcttgac aatacagtgg gggagcggtg aaatatgtct gaaaaccgta aacgataacg
    12841 tttgaacaac aaatcaacag aacaggaggg taacggcaaa cctgcccggc ttaccagcct
    12901 attctgggca aggagagaat actccacatt actgttcaaa cggctaaaac tgtttaaacc
    12961 gttcaaacag tttgacagag gaggatggcc tgccttccga agcctcggca taggcagacg
    13021 caggccccgg gaatagactg aaaggagaac agaccatggg aatgtacccg gagatagatg
    13081 ataagctgtt gagccttgaa gaagctgtag ggcgttttat caaaaatggt tcccagatcg
    13141 ccatcggtgg ttttacagtc accagaaatc caatggctct tgcctatgaa attgttcgac
    13201 agggcatcaa ggatattcac ctcgtatgcc actctcatgg ccaggccctc gatatcctga
    13261 ttggggcagg atgcgtaagc agacttgaga tagcctatgg tgggaacggc aggtacgctc
    13321 caacatgcat acggtttaag aaggcaattc agcgtggcga aattcagttc gaggattatt
    13381 cgaactacca gatgagcctc cgtttttttg ccggttcgct cggaatccca tttatcccga
    13441 caaaatcagg cctgggaagc gatctgatcg atatagaggg ttttcaccct gagatcagga
    13501 aagagaaaaa ggtggcttcg aaaaagtatg tggtcacgca gaatccattc aacggggagg
    13561 atgacaaagt gttgcttttg ccggcactta accctgacgt agccatcatg catgcccagt
    13621 atgtgggtga agacggtacg gttcggataa aaggcctcac atttgccgat atggagcagg
    13681 caaaatcagc tgacgtagtc attgttacct gcgaagagat tgtacccaga tcattcattc
    13741 gccttgatcc tgaccagaac tcacttccgc catttttcgt ggacgccatc gtgaaagtac
    13801 catacggcgc ccatcctacg gctgtataca cttactatga ttatgatcca aaacatctca
    13861 acctgtacaa aaaagtggct gaagatgaca aggcctttaa ggtctacctt gatgaatggg
    13921 tatatggcgt aaggtcacat caagagtatt tagaaaaagt agggaacact gcactttttc
    13981 tgataaaggc aaatcccatc ctcggatatg ctgtagggct tgacagaaaa tagggaggtt
    14041 ttacgatggc cgcatacacg gacaatgaaa tgatggccct cgctgcaggg cgtttcatca
    14101 ggaacggtga tatcgtattt gcagggacag gtgtgtcaat gcttgcagca acagcagcaa
    14161 aacggatcta tgcaccaaaa gcagtggtgt tttttgaaac aggcgggatt gatccatcat
    14221 tagaggaaat tcccatggct gttgcagatc ccaggatcat gtatggaacg tgcttaaatt
    14281 ctggcctcat agaagccttc tccattgttg gacaccgcaa gctgcacact attgcatttc
    14341 tgggcgcagc acaggttgat aaatatggaa acctcaacac aactgtaatt ggcgactatc
    14401 acaaaccaaa gacacgtttt tccggaagtg gtggtgcctg tgatgtggct tcattggcat
    14461 caggtgtaat taccttcatg caacaccaga agcggcggtt tgtagagaaa cttgactacc
    14521 ttacgagcgt tggttggtac aaaggagggg attcgaggcg aagacttggc cttccccggg
    14581 gcggggctct ggctgtggtc acaaacctcg gcgtaatgaa atttcacgaa acaacaagag
    14641 agatgtatct tgccgagtat tacccgggta tcactgtaga caagatcgtg gagaacaccg
    14701 gatttctcgt tgatacatcc cgtgcagttg aggcagctcc gcctactcct gaggaattga
    14761 ggatcctgcg ggaagaagtt gatccgcaga agctgatact ttaacaggca tttttttata
    14821 atatggaggt ttgaaagaca tggatatccc tgacaaccct gttcatgagg aactggaaaa
    14881 gctccccaaa gagacgctca ttgaactcat aaaaatgtat tcacgaaact ggcacacctg
    14941 cgacggtctc tggttctcag gggttgaaga gcagtacggt actgagaagg cgctcgaaat
    15001 tgacattaat atgtggagca tctcctcgcg gctcgaagca aaaaggatta aagaggttct
    15061 tgctatccct gataatgcag gccttgacga ggtattgaga gctatcaact ttatgagctg
    15121 ggctgccaaa tgttcttacc ggatagataa gaatggggat tcagcccttc ttaccgtgat
    15181 ttcttgtcca cctcaggaag cacgaatcaa agccggcaaa ggtgtattcg cctgcagacc
    15241 tactttcgaa ctcggttttg gtaacgttgc cagggtgatc gatccgagcg taaaggtgac
    15301 atgcaaatat tgccctcccg gcccttaccc agctgatagc tggtgtcagt gggaatttaa
    15361 ccgaaatcat gcaataccac ttgctttatg tgacataacc cgataacatt tcgcc
//
LOCUS       DAOOMC010000069        15214 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdus sp. isolate AMR_MDS_2685
            NODE_4164_length_15214_cov_6.238538, whole genome shotgun sequence.
ACCESSION   DAOOMC010000069 DAOOMC010000000
VERSION     DAOOMC010000069.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864760
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdus sp. (wastewater metagenome)
  ORGANISM  Syntrophorhabdus sp.
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae; Syntrophorhabdus.
REFERENCE   1  (bases 1 to 15214)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15214)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,729
            Genes (coding)                    :: 2,708
            CDSs (with protein)               :: 2,708
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 7 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15214
                     /organism="Syntrophorhabdus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4164_length_15214_cov_6.238538"
                     /isolate="AMR_MDS_2685"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2699756"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(55..621)
                     /locus_tag="PK125_08255"
     CDS             complement(55..621)
                     /locus_tag="PK125_08255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB38139.1"
                     /translation="MDNIEKKTRIIKEIKVDTKKCAGCRACEMACSAFHAVPRFSSVN
                     TARSRIMVVMDEVNDVFVPIRAGDYAQAECNGRNKYVIDGKEYAECTFCPASCPSRDA
                     FKEPDSGLPLKCDMCESDPPLEKPMCVEACTFGALTYVEREEVIPLVSVEESQEKRGE
                     MKIGLEMLIKKYGFRNVADTVARMGRKN"
     gene            complement(639..2597)
                     /locus_tag="PK125_08260"
     CDS             complement(639..2597)
                     /locus_tag="PK125_08260"
                     /inference="COORDINATES: protein motif:HMM:NF013481.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016625 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, iron-sulfur
                     protein as acceptor [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase N-terminal
                     domain-containing protein"
                     /protein_id="HPB38140.1"
                     /translation="MRYAETGFNLEIDLSKGSIDKVETDPKETALHLGGNGINAKLLF
                     DRVPPGTDPFSPENLLIFGNGLLNGTPVPGANRVSVDTIAPVNGLMGHSLMGGFFGAE
                     MKMAGYDRIVIRGRAPDLVYLAIHKNRVEIRDATHLRGKGIYDTTCLIKEELKDQRAW
                     VAAIGPAGENRVITASIDCGNSSASRTAGPVMGYKNLKAIAIRGTKDVNIAHPAELWE
                     ICSRLRKELDENPNVGDWMAVDEDDSFHHNNFSWGNARIRRKNFWSASLEERWRNLKY
                     DHMSRQTGCWNCPKACHNVIQWPGRRRFSYKCYGKDTYHMASFQELDFTYEILGIAQD
                     LGLDSYSTPQVIAFALELLEAGILKQSDFPGMPADVRGRFYYLIDKIAHREGIGDILA
                     HGTSGAAAIIGNGAEAFDHNTIKKFEQLPIKLGKLNPAYFLMIATGEDMAITQIEGSF
                     PQDPITDPELREEFIRKWVAVPDKKFIEWFRQWEKRDELPDDAMCAITDWNECMHYLD
                     DSLGFCGFVSSFRGQFGGTTGYHVWNMPEVITHATGMEFDKDRLWECFMRNRNLIRAL
                     NARLGLRRYMERPPEDHWAVRNEEYEQQLLTKYYDFKGWTFDGIPTKETLEKLGLGYV
                     AEDLIKRGVLTGEEVTALNDPRANKDKE"
     gene            complement(2664..2870)
                     /locus_tag="PK125_08265"
     CDS             complement(2664..2870)
                     /locus_tag="PK125_08265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB38141.1"
                     /translation="MFFRVYCNIKEIEAPDLETAEDILRNNLKEVIWLIPVTETSVAP
                     PAREEKKIEVKDKIGGHGSMTMRH"
     gene            complement(2939..3733)
                     /locus_tag="PK125_08270"
     CDS             complement(2939..3733)
                     /locus_tag="PK125_08270"
                     /inference="COORDINATES: protein motif:HMM:NF013757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HPB38142.1"
                     /translation="MRSVYGTKMVNRMSPAFKRVPAIDKCFAILDLLARSKEPLGVSD
                     IASRLGLNKSTVFNIGHTLDDLKVIENQGDGKYIFGTRFYVLSNMAGGRSPFMKTVHP
                     YLEIINEKTKLSAFLGVRSDRRAILIDKVDSAYGVKVSSEIGTQMPVLAGAGIKAMLS
                     QLSDEQIDEILGRTELKRFTPYSIVDKRAYKNEILQVRKDGIAYDREEYIEGMVAFGI
                     PIRAYGKNVQAAIWAVGLSSQITKDSLSGITELLGGIREEINCRIR"
     gene            4036..4725
                     /gene="phoU"
                     /locus_tag="PK125_08275"
     CDS             4036..4725
                     /gene="phoU"
                     /locus_tag="PK125_08275"
                     /inference="COORDINATES: protein motif:HMM:TIGR02135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HPB38143.1"
                     /translation="MEVMEGHIYRAFDLELKELKDKLLLEAGLVERAIQDAIKALLER
                     TSDVAKRVIEDDDSVNAIEVEIDEFCLKLLALRQPAARDLRFITTAIKINYDLERIGD
                     MAVNICERVLELNQEPQLKPYIDLPTMASIVQIMVKESLDAFLKEDVQLALKVALDDE
                     KVDSLLDQIFRELLTYMMQDARTISRATRLLFISKNLERIADHAVNIAELVIFMVEGK
                     IIRHLKPIPEE"
     gene            4732..5547
                     /locus_tag="PK125_08280"
     CDS             4732..5547
                     /locus_tag="PK125_08280"
                     /inference="COORDINATES: protein motif:HMM:TIGR02136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein"
                     /protein_id="HPB38144.1"
                     /translation="MPKLMGTVLLLILTLPLAFGACSSKDVQAKKRTVTVAGSTSVMP
                     FTEKLAEHFMIDHRDFIVEVQGGGSTAGIQACLNGTVNIGMSSRQLKGEENILNQIII
                     CYDGISLVLNPANPITNLSLKDVRDIFSGKIKNWKELGWIDRSIDAVTREEGSGTRGS
                     FEELVMTDEEIDDGIMVQDSNGSVKEVVATDPYAIGYISLGLVDDKVKAVIIDGVEPS
                     IDTIKLGQYKIVRPFLYVTREGLDDSAREFIDFVLSAAGQNVLKMEGLISVHD"
     gene            5540..6403
                     /gene="pstC"
                     /locus_tag="PK125_08285"
     CDS             5540..6403
                     /gene="pstC"
                     /locus_tag="PK125_08285"
                     /inference="COORDINATES: protein motif:HMM:TIGR02138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HPB38145.1"
                     /translation="MIKKALTKEHFVKYILTAFALSSLLFLFLIFIFILSEGIPLFHK
                     VGLKNIIFGFKWAPTKGSFGIFPMIVSSFLVTFGALMVGAPLGLSCAIYLSEYSGKKL
                     KLFLKPALELLAGIPSVVYGFLGVIYIVPLVRNFLGGPGFSLLATSIILGVMIFPTVI
                     SISFDALMSVPKTYREGSFAMGATKWQTIYKVIIPSARSGILASFILGMGRAIGETMA
                     VIMVAGNALRIPTSILDPLRTLTSNIALELAYATGDHRLGLFSTGVVLLVIIMILNYI
                     ATFAIRRRVLK"
     gene            6400..7236
                     /gene="pstA"
                     /locus_tag="PK125_08290"
     CDS             6400..7236
                     /gene="pstA"
                     /locus_tag="PK125_08290"
                     /inference="COORDINATES: protein motif:HMM:TIGR00974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HPB38146.1"
                     /translation="MRISPNFVDKIVKIGLNFQAFFTVGILIAIVAVIFFKGSPYLNL
                     EFIFSSPEDMGRHGGIFSTIIGTIFLVLLSIFIATPLGVGTAIFLTEYTKESWFTKII
                     RFGVESLAGIPSILYGLFGFIFFVVKLGMGWSLMAGVLTITIMILPTIIRTSEEAVKT
                     VPRNLRIVSYSLSATKWETVTKVVLPSAGPGILTGIMLSVGRAVGETAAVIFTMGSSL
                     RLPTSLMDSGRTMAVHFYILAREGISMEKAYATALVLVLSILFINIIAYYIMNKVTSK
                     YS"
     gene            7241..7996
                     /locus_tag="PK125_08295"
     CDS             7241..7996
                     /locus_tag="PK125_08295"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HPB38147.1"
                     /translation="MDIKIRTEELRLSFGKREILKGVTVDVYKNKITGFMGPAGSGKS
                     SLISIMNRMIDFEENATISGRVFIDGEDILDGEIDEVHLRRRVGMVFAVPIPLPRSIF
                     DNIAYGPRLKNKNGSRELRNLVQESLKKAFLWDEVKDRLNSSALKLSGGQQQRLCLAR
                     TLALEPEIILLDEPCSGLDPISTAKIEEALNELKKEYTIVLVSNNTKQIARITDYAAF
                     FYMGTLIEYGTTEKVFTNPSERKTEDYIQGKFG"
     gene            7996..8748
                     /gene="pstB"
                     /locus_tag="PK125_08300"
     CDS             7996..8748
                     /gene="pstB"
                     /locus_tag="PK125_08300"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004810563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HPB38148.1"
                     /translation="MNDHVLRTDKLNLFYGNFHALIDIDFHGFEKSITALIGPSGCGK
                     STLLKCFNRMNDLIDDVKTTGGIFVNERRIDEIDIIKLRKKVGMVFQRPNPFPFSVYE
                     NMVYGLKIHGLSNKKRNEETMERCLKAVGLWDDLGDRLQTSALSLSEEMKQRLCIARL
                     LTVEPEIILLDEPCSALDPIATMRVEELMMDLKKDYTILIVTHNMQQAARVSDYTGFM
                     LLGELVEFGETSQIFTSPKDARTEGYITGRFG"
     gene            8882..10099
                     /locus_tag="PK125_08305"
     CDS             8882..10099
                     /locus_tag="PK125_08305"
                     /inference="COORDINATES: protein motif:HMM:NF015418.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HPB38149.1"
                     /translation="MEFLKAVTAKEALEMINSFPISLHTEVIPLDESSGRIIAEDIIA
                     HENIPPFSRSLVDGFAVKVKDIQGAKETNPALLIYKGESRIGEETKETLEDGCCIYIS
                     TGAMIPEGADGVVMHEYSRPMGDAVEITRTIHKGENIIFPGEDIRTGSIVLKQGKRLT
                     PFDLGVLAALGIADVWSYKKPKIGLISSGDEIIPVDQKPAPGQIRDINHYTILNMLRK
                     TCSDVIFIGITGDNPEDIQGHLHAARDCHMILVSGGSSKGEKDYMTESIKQLGGKILF
                     HGVNIKPGKPTIFASLWEKPIFGLPGHPVSCIMALTRFVLPLVKRLQGDNNPEKKQTK
                     GVLTTNIPSSYGIEEYVRVEIDYDNKGCTVTPLFAKSSVISSLSRASGYIIVPEAKEG
                     FERGENVVVYYFE"
     gene            10373..12283
                     /locus_tag="PK125_08310"
     CDS             10373..12283
                     /locus_tag="PK125_08310"
                     /inference="COORDINATES: protein motif:HMM:NF011068.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032324 - molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin biosynthesis protein"
                     /protein_id="HPB38150.1"
                     /translation="MKRYLDTIKSTEGIRRILEQVKPIEDEEYLPSHASRGRITSRPV
                     IARASNPPFLCSAMDGYAVSFGNTLEADLGNPVSLVVGYDAFPINTGDPLPGNTDAVI
                     MVEEVEQTDEKIIIRKPSYLWQNVRMIGEDIIEGDMLFPANHKIRTLDTGMLLSAGIT
                     HIYVRRKPSVLIIPTGKELIDIYSDQYKDTKISGLVDFNSLLLVNMAEEMGFKAEKSN
                     IIINKEELSSTVNQASQLFDVIIINAGSSAGTEDFTELVIRELGTLLFHGVSMMPGKP
                     TLFGIVNGKPVFGLPGYPVSATTSFKIFFEPLVEKLMSTSITKELISCITPYKIPSRI
                     GIEEIIRVNIIGKDDQYFAFPLPRGASIFSSLANADGLVRVPENIEGFGEGEVIDCEL
                     LRSKEELNKRTHIIGSHDLSLGILRDMLKNRFPDQDLISTHVGSMSGIMAFRKGIADL
                     CTTHVLDEYDKVYNINVVNKYVPHRNWLLINIAKRTQGLIVQKGNPKSLSGIKDIAQE
                     GITFVNRQVGSGTRILLDAMLKENNIPDGSIKGYEREESSHNAIAILVREGVADTGIA
                     IHAVARAFSLGFIPLAQEDYDLLVTKEFSETERFESLLTLIRSDEFKNRLHEVGGYIT
                     EDTGKIKYVHKV"
     gene            12373..13347
                     /gene="moaA"
                     /locus_tag="PK125_08315"
     CDS             12373..13347
                     /gene="moaA"
                     /locus_tag="PK125_08315"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: protein motif:HMM:TIGR02666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HPB38151.1"
                     /translation="MLMDKYNRVINYIRISVTDKCNLRCKYCVNENFPHIPHPEILRY
                     EEIIRFVRLCAELGVTKVRLTGGEPLIKQDIEFLLKEINQIEGINDMSLTTNGVFLGE
                     KIHRLKEAGLKRVNISLDSLKPERFAYITGVDAYDRVLESISLAKDLGLNPVKINTVM
                     IKGFNDDEILDFARFAKVWDVQVRFIEFMPFGESTLWNSSRIIPSGEVEELIKKAYQI
                     KPSRNSHKGPAKMFEFEGSLGQVGFISPMSTHICSECNRIRLTSTGMIKPCLFSDTEY
                     DVKRLLRENAPDDVLKRFISDCVVAKPERKYEIGLIKKCQKSLISIGG"
     gene            13402..13887
                     /gene="moaC"
                     /locus_tag="PK125_08320"
     CDS             13402..13887
                     /gene="moaC"
                     /locus_tag="PK125_08320"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001612668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HPB38152.1"
                     /translation="MKLTHIDDEGNARMVDITEKKETQREARAYGQIKMSAGTYSVIK
                     TGKGPKGDIFTVAKIAGIMATKKTHEIIPLCHPLSLTHVDVDYSFDDRKHVVGITSTV
                     RTKGQTGVEMEAINCAMIVALTIYDMCKAVDKGIELGPFYLLEKSGGKSGKFVRKFRN
                     A"
     gene            13916..14353
                     /locus_tag="PK125_08325"
     CDS             13916..14353
                     /locus_tag="PK125_08325"
                     /inference="COORDINATES: protein motif:HMM:NF015438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HPB38153.1"
                     /translation="MTGRIISVNVSKNKGEKKHNIEKCMLIKDMGLKEDAHAGFMHRQ
                     VSLLAQESIEKIRKMGLHVVPGDFAENLTTKGIDLPSLPIGTRLLVGNDILLRVTQIG
                     KECHERCAIFQQVGDCVMPREGIFAEVIAEGEVQVGDTITVEL"
     gene            14350..14853
                     /locus_tag="PK125_08330"
     CDS             14350..14853
                     /locus_tag="PK125_08330"
                     /inference="COORDINATES: protein motif:HMM:NF013183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MogA/MoaB family molybdenum cofactor
                     biosynthesis protein"
                     /protein_id="HPB38154.1"
                     /translation="MSYTAAIVTVSDKGSVGEREDKSGPALRAALEGLYDVRETIVVP
                     DEVDIIAQTIRHLIDIKSIDLILTTGGTGVTKRDVTPEATKLVLERELPGFAEIMRME
                     SYKITPTAMVSRAISGTRKGSLVINLPGSPKAAVECLGFVLKAIPHTLDKLQGDTTDC
                     AALRENQ"
BASE COUNT         4307 a         3360 c         3371 g         4176 t
ORIGIN      
        1 cctcatcgat tacttctttg aagggtgcta gggtttccac gttttatcct cgtattaatt
       61 cttccttccc atccgggcaa cggtatcagc tacattcctg aatccgtact tcttgatcaa
      121 catttccagt ccgattttca tctcaccacg tttctcctga ctttcttcaa cagagaccag
      181 aggtatcact tcttccctct ctacataggt aagcgcaccg aaggtacagg cctcaacaca
      241 catcggcttt tccagtggtg ggtcactttc acacatgtca cacttgagcg gcaaccctga
      301 atcaggctct ttgaaggcat ccctcgatgg gcatgatgcc ggacagaaag tacattcagc
      361 gtattccttt ccgtcaatca catatttatt tctcccgttg cattctgctt gtgcatagtc
      421 tccggctcgt atgggtacaa acacatcatt tacttcatcc ataacaacca ttatccggga
      481 acgtgctgta ttgactgagc tgaaccttgg aactgcgtgg aaggcagagc aagccatctc
      541 acacgccctg cagcccgcgc atttttttgt atcaaccttt atctctttga ttattcttgt
      601 ttttttttca atgttgtcca cgatgacgct cccttctctc attccttgtc tttgtttgcc
      661 ctcggatcat taagggcagt gacttcttcg ccagtaagaa cacctctctt aatgaggtct
      721 tccgctacgt aacccaggcc caatttctca agggtctctt tggtgggaat cccatcgaag
      781 gtccaaccct taaaatcata gtacttcgtg agaagctgct gttcgtactc ttcattcctt
      841 acagcccaat ggtcttcagg tggtctttcc atgtatcgtc tcaggcccag tctcgcattg
      901 agcgctctga tcaggtttcg gtttctcatg aagcattccc ataacctgtc tttgtcgaac
      961 tccatccctg tcgcatgggt gataacctca ggcatgttcc agacgtgata ccctgtggta
     1021 ccgccaaact gaccgcggaa tgaggataca aatccgcaaa atcccaggga atcatccaga
     1081 taatgcatac attcattcca atcagtaata gcgcacatgg catcatccgg caattcatca
     1141 cgtttttccc attgtctgaa ccactctata aattttttat ctggaaccgc cacccacttt
     1201 ctaataaact cttccctcaa ctccgggtcc gtaattgggt cctgtgggaa cgagccttct
     1261 atctgagtta tagccatatc ctcaccagtg gctatcataa ggaaatatgc cgggtttaac
     1321 ttaccgagct taataggcaa ctgctcgaat tttttgattg tattgtggtc aaatgcctcg
     1381 gctccattac cgattatagc tgctgcccct gatgtcccat gggccagaat atctccaata
     1441 ccttccctgt gggctatttt gtcaatgaga tagtagaacc ttcctctcac gtctgctggc
     1501 attccaggaa agtcactttg tttaagaatt cctgcttcca aaagttcaag ggcaaaggcg
     1561 attacctgag gtgtagagta cgagtcaagc cctaagtcct gagcaatacc aagtatctca
     1621 taggtaaaat cgagctcttg gaaggaggcc atgtgatatg tgtcctttcc gtagcactta
     1681 taggagaacc tcctgcggcc cggccactga ataacgttgt ggcatgcctt cgggcagttc
     1741 cagcaacctg tctggcggct catatggtca tatttgaggt tccgccacct ctcttcaaga
     1801 ctggcgctcc agaaattctt acggcgtata cgggcattac cccaggaaaa attgttatga
     1861 tggaaactat cgtcttcatc aacagccatc cagtcaccaa cgttggggtt ttcatcaagt
     1921 tcttttctca gcctcgaaca aatctcccag agttctgcag gatgggcaat gttaacatct
     1981 ttagttccac ggatggcaat cgccttcagg ttcttgtatc ccataaccgg ccctgccgtt
     2041 cgtgaagcac tcgaattgcc acaatcaatc gaagctgtaa tgaccctgtt ttcgccagcc
     2101 ggaccaatgg cagccaccca ggccctttgg tctttcaatt cttccttaat caggcaggtg
     2161 gtatcatata ttccttttcc ccgaaggtgt gtcgcatccc ggatttcgac cctgtttttg
     2221 tgaatggcca gatagaccag atctggtgcc ctgccccgaa tgactattcg gtcatatcct
     2281 gccattttca tttctgcccc aaaaaatccg cccataagtg aatgacccat cagtccattc
     2341 acaggtgcaa tcgtatcaac tgaaacacga tttgcgcccg gaactggtgt gccgtttaaa
     2401 aggccattgc caaatatgag caggttttca ggagaaaatg gatcagttcc cggaggaact
     2461 ctatcgaata gaagctttgc gtttatgcca ttaccaccca aatgcaatgc cgtttccttc
     2521 ggatcggtct ctaccttgtc aatgcttcct tttgataaat caatttctag gttaaaccct
     2581 gtttccgcgt acctcatttt tacctctctc agtctcctcg caatcctgtg tgcaggccgc
     2641 tgaggttgtc aatatttcag gttctaatgg cgcatggtca tcgatccatg tccaccgatc
     2701 ttatctttca cttcaatttt tttctcttct ctggctggag gtgctacaga agtctctgta
     2761 accggaatca gccagataac ctctttcaaa ttgttcctga gaatatcctc tgcagtttcc
     2821 aggtccggag cttcaatctc ctttatatta caataaaccc taaaaaacat gtcctatcct
     2881 cctgcgatca ctgaatgaat ataaaaaaat tatcttgtct accactatgc tcttttcttt
     2941 atcgtattcg acaattaatc tcttcacgaa tgcctccaag tagctcagtt atcccggaaa
     3001 gggagtcctt tgttatctgg ctcgatagac ccactgccca tatggcagcc tgaacatttt
     3061 ttccatatgc cctgataggt atcccaaagg caaccatccc ttcaatatac tcttccctgt
     3121 catacgcaat accgtctttg cgaacctgga gaatctcatt tttgtaagct ctcttatcta
     3181 caatagaata cggggtaaat cttttgagct ccgttcggcc aaggatttcg tcgatttgct
     3241 catcggataa ctgggataac attgccttta ttccagcacc tgccaaaacc ggcatctgcg
     3301 tacctatttc agatgagact tttacaccgt acgcagaatc aactttgtca atcaatattg
     3361 cccttcggtc cgaacgaacc cctaaaaagg cagacagttt tgttttctca tttattatct
     3421 caagataggg atggactgtc ttcatgaatg gtgatctccc gcctgccatg tttgacaaca
     3481 cataaaaacg tgtgccgaag atatattttc cgtcaccttg attctcaatt accttgaggt
     3541 cgtcaagtgt gtgccctatg ttgaacaccg tacttttatt caggccaagc ctcgatgcaa
     3601 tatcactcac tcccagcggt tcttttgatc gggccagcaa atccaaaatt gcgaaacatt
     3661 tgtcaatagc tggaactctt ttaaaagctg ggctcattct gttaaccatt ttagtgccat
     3721 acacgcttct catattggca tatcgttttc ctaagaaaac atatgttttt tatatcaaac
     3781 atagcggtat ttgtcaacaa aaaaatgcag taaaatcttt taaaatcaat atctttgagc
     3841 tttcgttatt ttaagttagt aatggtaatt tttgttcagt atcataacag ggatcacgtg
     3901 gtccactttc agacacactt accagcttta tcctaaacaa ccatctgtct tctgatacaa
     3961 ccgaggatat tttcttgtat tagggcaggg aaattactat aatctgtctg gtaaaattat
     4021 gattaggggg ccacaatgga agtaatggag gggcacattt acagagcatt tgatttggag
     4081 ttgaaagagc tgaaggataa gctccttctc gaggctgggc tcgttgagcg agccatccag
     4141 gacgctatta aagccctcct cgaaagaacc tctgatgtag caaaaagggt tatcgaagac
     4201 gatgactcag tcaatgccat agaagtagag attgacgaat tctgtcttaa actgcttgct
     4261 ctgcggcagc cagcagcaag agatctaagg tttattacga ctgccatcaa aataaattat
     4321 gaccttgaac gcattggtga tatggctgtt aatatctgcg aaagggtgtt ggaactgaat
     4381 caggaaccac agctcaaacc ctacatagac ttgcccacca tggcttccat tgttcagata
     4441 atggtgaagg aaagccttga tgcttttttg aaagaagacg tgcagttggc actgaaggta
     4501 gcccttgatg atgaaaaagt cgatagcctt ctcgaccaga ttttcagaga gttgctcact
     4561 tacatgatgc aggatgcgcg gacaatcagc agggcaaccc gcctgctttt tatttcaaaa
     4621 aatttggaaa gaatagctga ccacgctgtc aatattgcag aactagtgat atttatggtt
     4681 gagggaaaaa taatcagaca ccttaaacct atacccgagg aatagaattg tatgcccaaa
     4741 ctcatgggga ctgtattgct cttgattttg acattacccc tggcgttcgg cgcttgttct
     4801 tcaaaagatg tgcaagccaa aaagcgcacg gttacggttg caggatcaac gtcagtcatg
     4861 ccattcacag aaaaactggc agaacatttc atgatagatc accgggattt catcgtggaa
     4921 gttcagggcg ggggatccac agcagggatt caggcatgtt tgaatggaac agtaaatatt
     4981 ggtatgtctt cacgacagct gaagggtgag gaaaacatac tcaatcagat catcatttgc
     5041 tatgacggaa tttccctggt actcaaccca gcgaatccca ttaccaatct tagcctgaaa
     5101 gatgtaagag atatcttcag cggaaagatt aagaattgga aagaactggg ttggattgac
     5161 agaagcattg atgcggtaac aagagaagaa ggatcaggaa cacggggatc ttttgaagaa
     5221 cttgttatga cggacgaaga gatcgatgat ggaatcatgg ttcaggattc gaatggttca
     5281 gtcaaagaag tggtagcaac tgatccatat gccattggtt atatatctct gggcttggtt
     5341 gatgataagg taaaagccgt aattatagat ggtgttgagc caagcattga cacaataaag
     5401 ctcgggcagt acaagatagt aaggcctttc ctgtatgtga cccgtgaagg gttagacgac
     5461 agcgcaagag agtttattga ttttgtgctc tcagcagcag ggcagaatgt acttaagatg
     5521 gagggtctca taagcgtaca tgattaaaaa ggccctgaca aaagagcact tcgttaaata
     5581 tatcctcact gcctttgccc tatcatctct cctgtttctt tttctcatct ttatctttat
     5641 cctttctgaa ggcatccctc tttttcataa ggtggggcta aaaaatatta tttttggctt
     5701 caaatgggca cccacaaaag gctcttttgg aatttttccg atgattgtct cttcctttct
     5761 tgtaacattt ggcgcgctca tggtaggcgc ccccttagga ctttcctgtg ctatttatct
     5821 ttctgaatat tctggaaaga agttaaagct cttcctcaaa ccggctctgg aactcctggc
     5881 gggcatacct tcagtagttt atggcttcct cggggtcatc tacattgtcc ctctggtgag
     5941 aaacttttta ggtggaccag gtttctcgct tcttgctaca tccataattc tcggggttat
     6001 gatatttcct acggttatta gcatatcctt tgatgctctg atgagcgtgc ccaaaacata
     6061 cagggaagga tcttttgcca tgggggctac gaaatggcag acaatctaca aggttataat
     6121 cccttctgcc cggtcaggta ttttggccag cttcattctt ggtatgggca gagcaatagg
     6181 agaaaccatg gccgtaatta tggttgcagg aaatgctttg agaataccca caagcatcct
     6241 tgatccattg cggacactga cgagcaacat agcccttgag cttgcctatg caacaggcga
     6301 ccaccgttta ggcctttttt caacaggggt tgttctcctt gtcatcatca tgatattaaa
     6361 ttacattgct acctttgcaa taaggagaag ggtgctgaag tgaggatcag tcccaacttt
     6421 gtcgacaaaa tcgtaaaaat aggtttgaac ttccaggctt tctttacggt gggtatactg
     6481 atagctattg ttgcagtaat cttctttaaa ggatcgccct atttgaacct ggagtttatc
     6541 ttttcatcac ccgaagatat ggggcggcat ggcggcattt tctcaacgat tataggtacc
     6601 atctttctcg ttctcttgtc catctttata gccacaccgc tcggagtggg aacagcaatt
     6661 ttccttactg aatatacaaa agaatcttgg ttcaccaaaa taatccgttt tggcgtagaa
     6721 tcactggcag ggataccatc catactctac ggcctttttg gctttatttt ttttgttgtc
     6781 aagcttggca tgggctggtc actcatggcc ggcgtgctca ccataaccat catgatactt
     6841 ccaacaataa tacggaccag tgaagaggct gtcaaaacag taccaaggaa tctcaggatc
     6901 gtgagttact cattgagcgc aaccaagtgg gaaacagtaa caaaggttgt gctcccttct
     6961 gcagggccag gaattctgac aggtatcatg ctcagtgtag gcagggccgt aggtgagact
     7021 gccgctgtta ttttcactat gggaagctca ctgaggcttc ccacctcgct catggattct
     7081 ggaagaacca tggctgttca tttttatata ctcgcccgag aaggcatatc aatggagaag
     7141 gcttatgcta ctgccctggt acttgtcctt agcatactct tcatcaatat aatcgcttac
     7201 tacattatga acaaggttac ctcaaaatac tcgtaaagat atggatatta aaatcagaac
     7261 cgaagaatta aggttgtcat ttggaaaaag ggaaatcctc aaaggggtta ctgttgatgt
     7321 ttacaaaaac aaaattacag gttttatggg gcctgcagga agcggcaaat caagccttat
     7381 ttctattatg aataggatga ttgacttcga ggagaatgct acaatctcag gcagagtctt
     7441 tatcgatggc gaggacattc tggatggtga aattgatgaa gtacatctga gaagaagggt
     7501 tggaatggtc tttgcagtgc caatccccct accccgttcc attttcgaca acatagccta
     7561 tggcccaaga ttgaaaaaca agaacggcag tcgtgagctt aggaaccttg ttcaggaaag
     7621 ccttaaaaaa gcattcctct gggatgaagt gaaagacagg ttaaactcgt cagcactgaa
     7681 gctgtctgga ggacaacagc aaaggctctg ccttgccaga acactcgctt tggagccaga
     7741 gattatcctg ctcgatgaac cttgctcggg ccttgatccc atttccactg caaagatcga
     7801 agaagccttg aacgagctta aaaaagaata tacgatagtg cttgtctcaa acaacaccaa
     7861 gcagattgcc cgcataacag attatgctgc ttttttttac atgggaaccc ttatcgaata
     7921 cggtacgact gaaaaggtat ttacaaaccc ctcggagagg aagactgaag attatattca
     7981 aggcaaattc ggataatgaa tgaccacgtt ctcaggacag acaagctcaa tctcttctat
     8041 ggaaacttcc atgcattaat tgatatagat tttcacggct tcgaaaaatc tatcaccgcc
     8101 cttataggac catccggttg cggaaaatca accctgctta aatgttttaa ccgtatgaat
     8161 gaccttatag atgacgtaaa aacaactggc ggcattttcg tgaatgaaag aaggattgac
     8221 gaaatcgata taataaaatt gagaaaaaaa gtggggatgg ttttccagag gcccaaccct
     8281 tttccgtttt ctgtgtatga aaatatggtt tatggactga agattcatgg attaagcaat
     8341 aagaagcgca acgaagagac catggagcga tgcttaaaag cagtcgggtt atgggatgat
     8401 ctaggtgata ggttgcagac atccgcattg agcctttcgg aggaaatgaa acagaggctc
     8461 tgcatagcac gcctcctcac tgtggagccg gagatcatcc tccttgatga accatgttct
     8521 gcccttgatc cgatagcaac catgcgggtt gaagaactta tgatggatct caagaaggac
     8581 tacacgattc tcattgtaac ccacaacatg cagcaggcag cacgggtctc tgattatacc
     8641 ggctttatgc ttctcggaga acttgtcgag tttggggaaa catcccagat ttttacatca
     8701 ccaaaggatg cgagaacaga gggatatata acaggaaggt ttggataaga ctggcgagat
     8761 gggaagtaaa acagggtaag cataggacat aattttgaat tttggattct cggttgtaaa
     8821 ttgacaaacc ttttgcaatt caaaattcaa cattcataat tgagatcagg gtggctgctc
     8881 tatggaattt ctcaaggcgg tgacagctaa agaagcgtta gagatgatca actcttttcc
     8941 catctctctc cacacagagg tcattccttt ggatgaatca tcagggagga ttattgctga
     9001 ggacatcatt gcccatgaga atattccgcc tttttcccgt tctcttgtgg acgggtttgc
     9061 tgtcaaagta aaggatatcc agggcgcaaa ggagacaaac cccgcgctgc tgatctataa
     9121 gggcgaatca agaatcggcg aggagacaaa ggaaaccctt gaggatggtt gttgtatcta
     9181 tatcagtacc ggggcaatga tacccgaggg tgcagacggc gtggttatgc atgaatattc
     9241 gagaccaatg ggcgatgccg ttgaaatcac caggactatc cacaagggtg agaatatcat
     9301 cttccccggc gaggatatca gaacaggcag catcgttcta aaacagggaa agaggcttac
     9361 tccgtttgat ctcggtgtac tggctgctct gggcatagcc gatgtctggt cgtacaaaaa
     9421 accgaagatc gggcttatat catcaggcga tgagattatc cctgttgatc aaaagccagc
     9481 gcctggccaa atacgtgata tcaaccacta cactatcttg aacatgttaa gaaaaacgtg
     9541 ttcggatgtc atctttattg gaatcaccgg cgacaacccg gaagatatcc aagggcattt
     9601 gcatgctgcc cgcgattgtc atatgatcct cgtgtcaggt ggtagctcca agggggaaaa
     9661 ggattatatg acagaatcca ttaaacaact cggcggaaaa atactctttc acggtgttaa
     9721 tatcaagcct ggcaaaccga ccatatttgc atccctatgg gaaaaaccca tcttcggact
     9781 tcccggtcat ccggtttcgt gcatcatggc cttgacaaga ttcgttcttc ctctggtgaa
     9841 aagactccag ggagacaata atcctgaaaa aaaacagact aaaggggtgc tcaccactaa
     9901 cattccttct tcctatggta ttgaagagta tgttagagtg gaaatagatt atgataataa
     9961 aggttgcaca gttacaccgc tttttgcaaa atcttcagtt atttcttctc tgtcccgggc
    10021 atcaggttat ataatcgtcc ctgaagcaaa agaagggttt gaaaggggag aaaatgttgt
    10081 ggtctattat tttgagtaaa acagggactg aaaagttggg aatataaggc aagaacatgt
    10141 ttttttacaa aataaaatgt tcacctgtga gttttttgtt attctttgta atgtttaata
    10201 aaatttataa ctaaacactt ccctgccttt atcaatctgc tgtgaaaatc gatttttttg
    10261 ggcaaaatgt ttataatcat tgtgaatgga agaacaaaga atgaacagtc tggcccgagt
    10321 ttcaatcctg gcacaatcaa tctgatgctt atgacttcca ttggagacta ccttgaaacg
    10381 atatcttgat acaataaaaa gcacggaagg aatcagaaga attcttgaac aggtcaaacc
    10441 catcgaagat gaggaatatc ttccttcaca cgcaagcagg ggtcggataa cgagcagacc
    10501 tgttatagcc cgtgcttcaa atcctccctt tctctgttct gctatggatg gatatgctgt
    10561 ctcattcgga aacaccctgg aagcagacct gggcaatccc gtatctcttg tcgtcggata
    10621 cgatgcattt cccataaaca ctggagaccc attaccaggc aataccgatg ctgtcatcat
    10681 ggtggaagaa gtagaacaaa cagacgaaaa aatcatcatc cgcaagccat cgtatctctg
    10741 gcagaacgta aggatgatcg gtgaagatat cattgaaggc gacatgctct ttccagcaaa
    10801 tcacaaaata cgtaccctgg atacaggcat gcttctctct gcaggtataa cccacatcta
    10861 cgtgagaaga aaaccttctg tactcattat ccccaccggg aaagagctta ttgatatcta
    10921 cagtgaccag tacaaagata ctaagatctc gggactggtt gattttaatt ctttacttct
    10981 cgttaacatg gccgaagaga tgggatttaa ggctgaaaaa tcaaatataa tcattaataa
    11041 agaggagtta tcttcaacag ttaatcaagc ctctcaatta tttgatgtaa ttataataaa
    11101 tgccgggtca agtgccggga cagaggattt cactgagctg gtaattcgtg aacttggcac
    11161 ccttctcttc cacggtgtat ccatgatgcc gggtaagcca acactttttg ggatagtgaa
    11221 cggaaagcct gtattcggac tcccgggtta tccggtttcg gctacaacaa gctttaagat
    11281 tttttttgaa ccgctcgttg aaaagttgat gtctacctct attacaaaag agcttatttc
    11341 gtgcatcacg ccatacaaga taccatctcg aattggcata gaagagatta tacgggtaaa
    11401 catcattggt aaggatgacc aatactttgc cttccccctg ccacggggtg cgagcatttt
    11461 ctcatctctg gctaatgcag atggccttgt tcgagttcca gagaacattg agggatttgg
    11521 tgagggcgaa gtcattgact gcgaactctt aaggagcaag gaggaactga ataaaaggac
    11581 tcatatcata ggaagccatg acttgtccct tggaatcctg agagacatgc tgaagaacag
    11641 gttcccggac caggatctca tttcaaccca tgtaggaagt atgagcggca ttatggcatt
    11701 cagaaagggc attgcagatc tctgtacaac ccatgttctt gatgaatatg acaaagtgta
    11761 taatattaat gtagttaaca aatatgttcc tcacaggaac tggcttctca tcaacattgc
    11821 caagaggact cagggcctta tagtccagaa aggaaatcca aaatcgcttt cagggataaa
    11881 ggacatagca caagagggta taacatttgt gaacaggcag gttggatcag gaacgagaat
    11941 tctcctcgat gcaatgttaa aggaaaacaa catcccagac ggttcaataa aaggttacga
    12001 gagagaggaa tcttcccata atgctattgc aatcctggtc agagaaggag ttgctgatac
    12061 aggtatcgct atccatgcag tagcgagagc gttctctctt ggtttcattc cgctggccca
    12121 ggaggattat gatctgcttg taacaaagga gttttcagaa acagaaaggt ttgaatctct
    12181 ccttaccctc ataaggtcag atgaatttaa gaatagatta cacgaagtcg gtggttatat
    12241 tactgaggat accggaaaga ttaaatatgt acataaagtg taggaaatac aggtcactgt
    12301 tatcgatcca ccgtggagcg attcagtttc tgtattctgt agactgtgta ctgaaaacaa
    12361 ggaacaacaa ttatgttaat ggataaatat aacagggtta tcaactatat acggatatct
    12421 gtcacggaca agtgcaattt aaggtgcaag tactgtgtca acgagaactt ccctcatatc
    12481 ccccaccctg agattctgcg gtatgaggaa attatacggt tcgtgaggct atgtgcagaa
    12541 cttggcgtaa caaaagtaag gctgacaggc ggtgagcccc tcattaaaca ggatattgaa
    12601 ttccttctca aggaaatcaa ccaaatagaa gggataaacg atatgagtct cacaacaaat
    12661 ggggtcttcc tgggtgaaaa gattcaccga cttaaagaag ctggccttaa gagggtcaac
    12721 ataagccttg acagcctcaa accggagcgt tttgcctata ttaccggtgt cgatgcctat
    12781 gacagggttc ttgaaagcat ctcactggcc aaagacctcg gtttaaaccc tgttaagata
    12841 aatacagtca tgataaaagg attcaacgac gatgaaatcc tggattttgc tcgttttgcc
    12901 aaggtttggg atgtacaggt gagattcatt gaattcatgc ccttcggtga gtcaacctta
    12961 tggaatagtt caaggatcat accttccggc gaagtggagg aattgattaa aaaagcgtat
    13021 caaatcaagc catcaagaaa ctctcacaaa gggccggcta agatgttcga gtttgaagga
    13081 agcttgggcc aggtaggctt catcagccct atgtccaccc acatatgcag tgaatgcaac
    13141 agaattcggc ttacttcgac cggcatgata aagccatgcc ttttctctga cacagaatac
    13201 gatgtcaaaa gacttctgag agaaaatgca cctgatgacg ttctaaaaag gtttataagc
    13261 gactgcgttg ttgcaaagcc tgaaagaaag tatgagatag gactcataaa gaagtgtcaa
    13321 aaaagcttga taagtattgg cggttaacaa acacccgtga tgcgtcacgc gttccgggta
    13381 acgtgctttc aaggagagca gatgaagctg acccatattg atgatgaagg taatgccaga
    13441 atggttgata ttacagaaaa aaaagaaacc cagcgggagg cgcgggcgta cggacagata
    13501 aagatgtcag ccggcaccta ttctgtgatc aaaaccggca aaggccctaa aggagatatc
    13561 ttcaccgtag ccaagatagc gggcatcatg gccacaaaaa aaacccatga aattattccg
    13621 ctttgccacc ctttatctct aactcatgta gatgtagatt actcctttga tgacaggaaa
    13681 catgtcgtag gaatcacctc aactgttcgg acaaaaggac agaccggtgt tgagatggag
    13741 gcaatcaatt gcgccatgat tgtggcactc actatttatg atatgtgcaa ggctgttgat
    13801 aaaggcattg agcttgggcc gttttacctt ctggagaaaa gcggtggaaa gagtggaaaa
    13861 tttgtaagaa aatttcgtaa cgcatgacgc gttacggatt taggagcgag gtcatatgac
    13921 aggcaggatt atttcagtaa atgtgagtaa aaataagggc gagaagaaac ataacataga
    13981 aaaatgcatg ctcataaaag atatggggct aaaagaggac gcccatgcag gttttatgca
    14041 tcgccaggta agcctccttg cccaggagag catcgaaaaa atcagaaaaa tggggcttca
    14101 cgtcgttccc ggagattttg cagaaaacct cacaactaaa ggtattgatc tcccctctct
    14161 acccattggc actagattat tagtgggaaa tgacatcctt ctccgcgtca cccagatagg
    14221 caaggaatgt catgaacgct gtgccatttt ccagcaggtc ggggattgtg ttatgccaag
    14281 ggaaggcata tttgcagagg ttatcgcgga aggtgaagtg caagtgggag atacaataac
    14341 ggtggaatta tgagctatac agcagccata gttacagtca gcgacaaagg ttctgtgggt
    14401 gagcgggaag ataaaagcgg tcctgccctc agggcagccc ttgaggggtt atacgatgtc
    14461 agagagacaa tcgtcgttcc tgatgaagtc gacattattg cccaaaccat tcgacatctg
    14521 attgatatta aatccatcga ccttattcta acaacaggtg gaacaggcgt aactaagcga
    14581 gacgttacac cagaggcaac aaagcttgta ctggagaggg aattgcctgg atttgccgaa
    14641 ataatgagga tggaaagcta caaaataact cccacagcca tggtatccag ggccatcagt
    14701 ggaacacgta aggggagcct cgttatcaac ctgcctggca gcccgaaggc ggctgttgaa
    14761 tgccttggct ttgtcctcaa agccatcccc cacacccttg acaagctaca gggggataca
    14821 accgactgcg ctgctttgcg agaaaatcag taattcattt cgaaactttc ccatatgtga
    14881 aagaccgggt ttcccacaga tcatattttt gtacttttcc ggtttgccgg ttgtgccgga
    14941 agccagcggt ataggcctat tcctttgcac cccttctatc ctcctgggca aacaccaggg
    15001 aactgaggct aaggattaag actgccatta tctaacagat ggatgtttta tattttttca
    15061 tgatctgtgc ctcctgtagt tatttgctgg cgccgggggt gagcaatgtc ggcttgcatg
    15121 aattgtctgg ctgctgcaag ttacttctgt gggttatcac gacggaaaag ctcccactcc
    15181 tcaacaaccg taccgtcagg cagatggcac atac
//
LOCUS       DAOOMC010000070        15098 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdus sp. isolate AMR_MDS_2685
            NODE_4200_length_15098_cov_6.462807, whole genome shotgun sequence.
ACCESSION   DAOOMC010000070 DAOOMC010000000
VERSION     DAOOMC010000070.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864760
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdus sp. (wastewater metagenome)
  ORGANISM  Syntrophorhabdus sp.
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae; Syntrophorhabdus.
REFERENCE   1  (bases 1 to 15098)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15098)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,729
            Genes (coding)                    :: 2,708
            CDSs (with protein)               :: 2,708
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 7 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15098
                     /organism="Syntrophorhabdus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4200_length_15098_cov_6.462807"
                     /isolate="AMR_MDS_2685"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2699756"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..919)
                     /locus_tag="PK125_08335"
     CDS             complement(<1..919)
                     /locus_tag="PK125_08335"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: protein motif:HMM:NF011456.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HPB38155.1"
                     /translation="MKKFNVAVVGATGAVGNEMVATLEQRKFPIKKLTLLASARSLGK
                     TLTFKGEEIPVDELKEDSFKGIDIGLFSPGGAVSTKFAPIAVASGCVVIDNTSAFRME
                     PDVPLVVPEVNEHAISQYKKRGIIANPNCSTIQMVVVLKPLHDAAKIKRVVVSSYQAV
                     SGTGKKAIYELEQQVLAIYNNKKVENKVYPWQIAFNVLPHIDSFLENGYTKEEMKMVN
                     ETKKIMEDDNIKVTATTVRVPVFYGHSEAVNIEFEKDMSPEKARKLLRKAPGVKVVDD
                     PAKNKYPLAIHAAGKDDTFVGRIRKDESVP"
     gene            complement(906..1406)
                     /locus_tag="PK125_08340"
     CDS             complement(906..1406)
                     /locus_tag="PK125_08340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HPB38156.1"
                     /translation="MRLKGRVWKFGDNIDTDVIIPARYLSSTDPTILGDHCMEPLDPS
                     FSKKVKKGDIIIGGFNFGCGSSREHAPIAISALGIPLIIAKSFARIFYRNAFNKGLAL
                     LEADIFDEIDQGEEIVVDLSTGIIKWGLNEVKAKSIPPFMVELVNEGGLINYLRKKLE
                     EENEKI"
     gene            complement(1403..2665)
                     /gene="leuC"
                     /locus_tag="PK125_08345"
     CDS             complement(1403..2665)
                     /gene="leuC"
                     /locus_tag="PK125_08345"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HPB38157.1"
                     /translation="MGMTLTEKILAAHAGKEYVEPGEIIEAKLDLCLANDITAPLSLE
                     EFDRVGAKKVFDPEKVVFVLDHFTPNKDIPSAENCKKIREFSKKFSINHVYDGGACGI
                     EHALLPEEGLVVPGDVVIGADSHTCTYGALGAFSTGVGSTDLTYGMVTGKNWFKVPES
                     IKFHCTGKWQKWVTGKDLILNIIGMIGVDGALYASMEYDGDAISALSMDSRFAIANMA
                     IEAGAKNGIFRADEKTLAYVNGRGKRPFTVFESDSDAKYREIYEINVGELDLKVACPS
                     LPSNVKDAKELYNIRIDQVVIGSCTNGRIEDLAMAATVLKNKKVAQDVRCIVIPATPK
                     IYLEAMRRGYLEIFMEAGCIISPPTCGPCLGGHMGILAKGEKAIATTNRNFVGRMGHP
                     ESEVYLSGPAVAAASAVKGLIIHPSEVV"
     gene            complement(2668..4197)
                     /locus_tag="PK125_08350"
     CDS             complement(2668..4197)
                     /locus_tag="PK125_08350"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: protein motif:HMM:NF002087.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HPB38158.1"
                     /translation="MADRVFLFDTTLRDGEQSPGNTMNTPEKLRVAKQLEALGVDIIE
                     AGFPIASEGDFEAVRLIAESIKNSEVAGLARANDEDIDRAWEAIKVAKKPRIHTFIST
                     SDIHLRHQFKKNKEEILNLAVNAVKRAKRYTDNVEFSAMDATRSDRDYLCKVFEAVID
                     AGANTLNIPDTVGYAVPDEFGGLIRYIKTNVPNISEAIISVHCHNDLGLAVANSIAAI
                     QNGARQIECTVNGIGERAGNASLEEIVMILRTRKDLFGLDTRVKTEKIYRTSRLITSI
                     TGVPVQPNKAIVGANAFAHESGIHQDGLLKSKITYEIMTPKSVGIPKSSLVLGKHSGR
                     HAFRERIIEMGYSLEDKEINLAFKRFKALSDIKKDIYNEDIEMIIMDEIYKIPEKYEL
                     VYLNVTCGKATIPTATVKIDIDGTTYQDVGVGVGPVDATYKIIKKLVKTNSKLLRFSI
                     NSITQDMDAQGEVFVKLEEKGHAVIGKGADTDIIVASAKAYINGLNKLEYLKGKKTGD
                     I"
     gene            complement(4292..5392)
                     /gene="lpxK"
                     /locus_tag="PK125_08355"
     CDS             complement(4292..5392)
                     /gene="lpxK"
                     /locus_tag="PK125_08355"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: protein motif:HMM:NF014645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HPB38159.1"
                     /translation="MKDIVIKFWKGEARYWKWFLLLPFICLSKLYGLCLHLRNALYDS
                     GLLKIDEVPIPVLAIGNITIGGTGKTPLVERLGYRMKEIGFNPGIITRGYKRKKKGIF
                     CVDKDNDKAKDVGDEALMLAKKTKIPVFVGKRRSLAIVEAMRKNNINLAILDDGYQVK
                     NILKNVDIVVLKSDQYNKSTNLFPLGPCREPIIRLKDADAILVNDGSIEPEIADVIYG
                     IPTFRMTYRPMHLYNVKHNLSTHYNILNGRKVLAFSGLGDNRSFFELVRALGADVVHE
                     VAYEDHYTYKNKDIEELSSYGDISLIVTTEKDAVKMIGMPVPDNLFYLSIEVNIEKEQ
                     ELIDVILKKIQAAAVPMPAMGNGIRAQKYWIH"
     gene            complement(5463..5795)
                     /locus_tag="PK125_08360"
     CDS             complement(5463..5795)
                     /locus_tag="PK125_08360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB38160.1"
                     /translation="MQKQSKESITPIERHETIRQYIVALLQDYTISAKELSNFLKIPE
                     KDVHDHLVHIKKTMNKGSYHLVVYPAECSKCSFVFRKRDKLAKPGKCPICHCNQIIPP
                     LFSIIKYT"
     gene            complement(5832..6710)
                     /locus_tag="PK125_08365"
     CDS             complement(5832..6710)
                     /locus_tag="PK125_08365"
                     /inference="COORDINATES: protein motif:HMM:NF020252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HPB38161.1"
                     /translation="MMEDFESKIKQTIEYLPPIPSVMAELIQALNDDIDLNDLGKIIS
                     KDPSMSMNVLKVANSAFYGLRNKVATIEHAVRMLGTREITSLCIACSACSILRPSSRQ
                     PTIDLIMFWRHSVATGVFSKLLSSELNIGLLNNIYLAGLIHDVGKIVLDKFAHDMYTD
                     VLRLARGQNISVLEAEKRVIGASHDMVGAWLMEKWQLPIIFSQVAMYHHQTYKAPDEC
                     LIAVSIVSFANHISKLKSFCFGGEIDGTTLRETDAFKILERINPGVRGLDFLDFIESL
                     NGAREEIVNIERVINV"
     gene            complement(6856..7740)
                     /locus_tag="PK125_08370"
     CDS             complement(6856..7740)
                     /locus_tag="PK125_08370"
                     /inference="COORDINATES: protein motif:HMM:NF020252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HPB38162.1"
                     /translation="MIIKDFKSKVLKMISYLPPVPTVMVELVQALNNEDIELNVLGKI
                     IAKDPSMSVNVLKVANSAFYKLPVKVTTIDHAVRMLGTREIASLCIACTAGKTLKAPA
                     NKATIDLSMFWQHSVATGVISKVLCNELGILTQNNIYLAGLIHDVGKIILDRFMHDIY
                     KEIVKITYNENISMIEAEQRVIGESHAKVGGWLMKAWNLPSIFVDIANYHHSVMETRE
                     ESRLEVAIVSLSNQLARLKSFGFGGDMSGVVLADIDAWKIIDEAYPQIKNLDVVKFIW
                     DLDEAYNEIVAMERIINN"
     gene            7919..9214
                     /locus_tag="PK125_08375"
     CDS             7919..9214
                     /locus_tag="PK125_08375"
                     /inference="COORDINATES: protein motif:HMM:NF019285.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside transporter C-terminal
                     domain-containing protein"
                     /protein_id="HPB38163.1"
                     /translation="MSKYNLISCFGILFFICFLALLSRQKRSISVRILFWGIGLQILF
                     AFFLFCIPVGTKFFLFLNDFAMGVIDSSFQGSQFLFGRLAIAPGKEGSIGFILAFQAL
                     PSIIFFAALMELLYYIGVMEKIIALFARIFIKVMGISGAEALCASAQIFLGIESNLTV
                     RPYLSKMTQSELLVVLTTGMATIASSVLGLYVIILSDSFPKVAAHLISASILLAPAAI
                     LASKLIIPETYKPLTLGTTVKIESQRAGNVIESIINGSMAGLKMVVGIGTLLIAFIGL
                     ISLIDVGLLFTGKLFSTVLGIRVDVTLHTILQYVFYPFTVAMGVPLVDAQTIARIIGE
                     RIIETEVKSYQDLALLIKEGKIVYERSIVIATYALCGFAHIPSLAIFVGGTASLVPER
                     TKDISILGVWALIASNIACFITGAVAGIFYTSGGSHFLK"
     gene            complement(9227..9694)
                     /locus_tag="PK125_08380"
     CDS             complement(9227..9694)
                     /locus_tag="PK125_08380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB38164.1"
                     /translation="MANFDEEMNKIDDSEDLLKLLESQEKKRPKKSSSVVPFMKSLPL
                     GTIVLVIVVVILGAMVLSLGSNMAVLKGELTDLKNVKGQISELEGKIEKSIREKDGLK
                     SEIAQLKNEINSSKVQRREAEAKAQQQKQKTEKKKAAPEKPKLSKPALPPPKR"
     gene            9850..10362
                     /locus_tag="PK125_08385"
     CDS             9850..10362
                     /locus_tag="PK125_08385"
                     /inference="COORDINATES: protein motif:HMM:NF025637.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HPB38165.1"
                     /translation="MDRTLLLNTTFEPLSVLSWKKAVTLIFLGKVEVLKEYEREIKGV
                     SRSIRQPAVIRLVRLVRNNHVSVKFSRRNIFLRDDFTCQYCGKRHDPKGLTCDHIIPK
                     SRGGATEWTNIVTSCIRCNHKKGDKLPEEVDMLPRKRPSRPSGFYMLTLHLGVKSLPD
                     YWRDYLFLRD"
     gene            10364..10852
                     /locus_tag="PK125_08390"
     CDS             10364..10852
                     /locus_tag="PK125_08390"
                     /inference="COORDINATES: protein motif:HMM:NF013401.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT domain-containing protein"
                     /protein_id="HPB38166.1"
                     /translation="MDRIWAPWRMEYITASSTSDNRCFLCVDSSRDDQALVVGRNNTA
                     FIIMNRFPYTNGHVMVVPIRHVGSIDSLTDEETLDMMRLTKIMVAIFKKEFSVDGLNI
                     GMNLGRAAGAGLEEHCHIHIVPRWFGDTNFMPIFGETRVISEHLHASYERLKRRFLEQ
                     SF"
     gene            10836..11585
                     /locus_tag="PK125_08395"
     CDS             10836..11585
                     /locus_tag="PK125_08395"
                     /inference="COORDINATES: protein motif:HMM:NF013232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB38167.1"
                     /translation="MSSLFDTSPPVIKPADIVRTFTGKSQEDLQLPRRAIITFNAGDV
                     KALLTGGCQKPVKAWENFRTLFRIAQSNTIVTRSFFGGPNIAALVEELSAFGVREFLL
                     WGYCGGIADDISIGDLLIAEGALRQDGLSYHYLSDNDDFVYSSWFPKWRKAAIASQII
                     PSLIWSCDALYRETRKKINLHKKMGMHAVEMEVASFYAVCKAKRLKGIAFLVVSDLLR
                     KDKWKPGFSKESFKKGARRLKEFLLEQGILR"
     gene            complement(11621..13765)
                     /locus_tag="PK125_08400"
     CDS             complement(11621..13765)
                     /locus_tag="PK125_08400"
                     /inference="COORDINATES: protein motif:HMM:NF015052.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 57 protein"
                     /protein_id="HPB38168.1"
                     /translation="MSRIHLAFLWHMHQPFYKNLFTGEYLLPWVLLHGTKDYYDMAYI
                     LKEFPGLKQNFNLVPSLLRQLMDYEDLQAKDVYLEIFRTPPRDLTESQKTYLLMNFFS
                     ANWDNMIKPFPHYFELLKKRGFYYPKEGIKEIKDYFTDEELRDIQIFFFLAWIDPMFF
                     EQYDGLKYLKSKGRSFSEDDKKIIQDVQKNILKSIIPLYRELYENSSIELSTSAYYHP
                     IIPLLIDSAAAREALPDAVLPQKLFRQREDASYQIKKAIDFFSELFGHRPRGMWPPEG
                     CVSNDSFALYAEHGVEWSVTDEEILFQSLGVNNTRDGYGNLTNPDLLYRPYIWEREGR
                     LLKVLYRDKQLSDLISFHYSRMASKDAANDCLRRLKEIRNSVKGRIRMPIITIALDGE
                     NAWENYANDGWDFLRYLYDDILKEESIVPTTISEYFDKTEDYGRISGTFAGSWIGHNF
                     SIWIGQVEDNAGWDLLSQTREFLQNEDPEMENEQAWESIYIAEGSDWFWWYGDDHSSE
                     NDEVFDFLFRENLANVYRFSGKEPPEVLAIPILLEDQEIKPTREPIDFIYPKIDGKVT
                     TYFDWIGSGFIEGKGHGVAMHDTALLIKGCYYGFNENSFFLRADIDRNFVENMGDVSF
                     EINLITESSTKIIYRVKDAIKEAPIQVSLAYADILELECPRKALDMKADEKINIWLSL
                     KVRDMMVDRLPKRGYVSVKVPSESFEMEMWYV"
     gene            complement(13765..14232)
                     /locus_tag="PK125_08405"
     CDS             complement(13765..14232)
                     /locus_tag="PK125_08405"
                     /inference="COORDINATES: protein motif:HMM:TIGR00040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HPB38169.1"
                     /translation="MKRIGVISDTHLSKPTDDFKYTLDKCFAEVEIVIHAGDMTGLLV
                     HDYLVKWDLKAVAGNMDDSDLCAILPEKRVEEIEGWKIGITHGRGSPFWLEQFVLDKF
                     ENVDIVIFGHSHIPLSTKKRNIHLFNPGSYKSSRTVGILELGEKISFNLIELR"
     gene            complement(14243..14950)
                     /locus_tag="PK125_08410"
     CDS             complement(14243..14950)
                     /locus_tag="PK125_08410"
                     /inference="COORDINATES: protein motif:HMM:NF024498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbC family protein"
                     /protein_id="HPB38170.1"
                     /translation="MLKRLIALLVVSLSLMFLPSLAMPQNVEESFKKLFPNIPVESIH
                     PTEIKGIYEIIVQNQIGYFAPEPGYLILGEIIDKDGTNITTKRRNENIVSKVRDLPLD
                     KAVKIGNGNSTIYEFTDPDCPYCRRASSFLSEKTDVTRYIFFLPLPMHKDAENKVKYI
                     FCSNDTAKAYEEAMHGKLDNKKYEICKKPEVDVLLKTHKDVSRKMGINSTPFFIVNGK
                     PISGANAPEIDKALQQK"
BASE COUNT         4124 a         3387 c         2859 g         4728 t
ORIGIN      
        1 atggcactga ttcatccttc ctgattctcc ctacaaaggt atcatccttt cctgctgcat
       61 gaatggcaag aggatattta ttctttgctg gatcatctac aacttttaca ccgggcgctt
      121 ttctgagtag ttttcttgcc ttttccgggc tcatatcctt ttcaaattcg atgttgacag
      181 cctctgagtg accataaaac acagggaccc ttactgtcgt tgctgtcacc tttatattgt
      241 catcttccat aatctttttt gtctcattta ccatcttcat ttcttccttt gtgtaaccgt
      301 tttcaaggaa agagtcgatg tggggtagaa cattgaacgc tatctgccac gggtaaactt
      361 tgttttcaac cttcttattg ttgtaaatag caagcacctg ttgctccaat tcatatattg
      421 ctttcttacc tgtaccggat actgcctgat agcttgaaac aacaaccctc ttgatcttcg
      481 ctgcatcgtg aagaggtttc aacaccacta ccatctggat ggtcgaacag ttgggattcg
      541 caataatacc tctcttttta tactgtgaaa tggcgtgttc gttaacctct gggacaacaa
      601 gggggacatc gggttccatt ctgaaagcgc ttgtgttatc aataacaacg cagccgcttg
      661 ctactgcaat aggtgcaaac ttcgtactca ctgcacctcc tggcgaaaaa agtcctatat
      721 caatcccttt gaatgaatcc tctttaagtt catctactgg gatttcttct cctttaaagg
      781 ttaatgtctt tccgaggcta cgtgctgatg caagaagtgt tagttttttt atgggaaatt
      841 tcctttgttc cagtgttgca accatttcgt ttcctacagc accagtggcg ccaacaacag
      901 ctacattaaa ttttttcatt ctcctcctcc agttttttgc gcaagtagtt tatcagcccg
      961 ccctcattca ccaactcaac cataaaagga gggattgact ttgccttgac ttcattcaaa
     1021 ccccatttaa tgatgccggt agaaaggtcc accacgattt cttcaccctg atctatctca
     1081 tcaaaaatgt cagcctcgag aagtgcgagt cctttattaa aggcatttct gtaaaaaatc
     1141 cttgcaaagc tctttgctat tataaggggt atacccagag cagatattgc gataggagca
     1201 tgctctctag aagacccaca accaaaattg aacccaccta tgataatgtc gccttttttg
     1261 accttcttag aaaaagatgg atcaagaggt tccatacaat ggtcacccag tattgttgga
     1321 tctgtagatg acaggtatcg tgcgggaata ataacatctg tatcaatgtt gtcgccaaac
     1381 ttccaaactc tgcctttcag tctcatacta cctccgaagg atggataata agacccttga
     1441 cggcactcgc cgcagcaact gcaggtcctg aaagataaac ttcgctctcg ggatggccca
     1501 tcctgccgac aaaatttctg tttgttgttg caatcgcctt ttctcccttc gccagaatcc
     1561 ccatatgacc accgagacat ggaccgcacg tagggggtga aatgatacat cctgcttcca
     1621 tgaatatctc caaataacct ctgcgcatag cttcaagata aatcttcgga gttgcaggaa
     1681 tgacaataca tcggacatct tgggcaacct ttttattctt caacacagtc gctgccatgg
     1741 caagatcttc aatcctccca tttgtacatg aaccgattac aacctgatct atacgaatat
     1801 tgtaaagttc tttcgcatct ttcacatttg aggggaggga tgggcatgct accttaaggt
     1861 ccaactctcc cacattgatc tcataaattt cccgatattt ggcatcgcta tcgctctcga
     1921 aaacagtgaa aggtctcttg ccgcgaccgt taacatacgc cagagtcttt tcatccgccc
     1981 taaaaatacc attctttgct cccgcctcaa tggccatgtt agcaatggca aaccgtgagt
     2041 ccatagagag ggcggaaata gcatcaccat catactccat tgaggcatac agagctccat
     2101 caacaccaat cattccgata atattcaaaa taagatcttt tcctgtgacc catttctgcc
     2161 atttgccggt gcaatgaaat tttatgcttt ccggcacttt gaaccagttt tttccagtta
     2221 ccatgccgta agtaaggtct gtgctcccga ctccggttga aaaggcaccc aaggctccat
     2281 aggtgcatgt gtgactgtca gcgcctatga caacatcgcc tgggaccaca agcccctctt
     2341 caggcaataa ggcatgttct ataccgcaag caccgccatc ataaacgtgg ttgatgctga
     2401 acttttttga aaactcccta atttttttac aattctccgc agagggaata tctttatttg
     2461 gagtaaaatg atcaaggacg aaaacaacct tctctggatc gaaaactttt tttgcgccca
     2521 ctctgtcgaa ttcttccagt gaaagcgggg cagtgatgtc attagcaagg cagagatcaa
     2581 gcttcgcttc aatgatttca ccaggttcca catattcctt tcctgcatgg gcagcaagta
     2641 ttttttcagt taatgtcatt cccataacta tatgtctcca gttttcttac ctttaagata
     2701 ttcaagcttg ttcaatccgt ttatgtatgc tttggcactg gccacaatga tgtcagtatc
     2761 agctccttta cctatcactg catgtccttt ctcttcaagt tttacaaaga cttctccctg
     2821 ggcatccatg tcttgcgtta ttgagttaat ggaaaatctg agaagcttgc tgttcgtctt
     2881 aaccaatttt ttaataatct tatacgttgc atcaacaggt ccaaccccta caccaacatc
     2941 ctgatacgtt gtaccatcga tatctatctt gactgttgct gttgggattg ttgccttccc
     3001 acaggtcacg ttgagataaa caagttcgta tttctcggga atcttgtaaa tctcgtccat
     3061 gatgatcatt tcaatgtctt cgttataaat atctttttta atgtcagaca gggctttgaa
     3121 tctcttgaat gcaaggttga tctctttatc ctccaaggaa tagcccattt ctatgattct
     3181 ttcacgaaac gcatgcctgc cggagtgctt tcctagaacc agagaacttt ttggaatacc
     3241 tactgatttt ggagtcataa tttcgtaagt aatttttgac tttaaaagtc catcttggtg
     3301 gatgcctgat tcatgtgcaa aagcattagc acctacaatg gccttgtttg gttgcaccgg
     3361 tacccctgta atagatgtaa tcaatctgct tgtgcgataa atcttctctg ttttaacccg
     3421 tgtatcaagg ccaaataaat cctttcttgt tcggaggatc attactatct cttcaaggga
     3481 tgcgtttcct gccctttctc cgataccatt tacagtacat tctatctggc gagctccatt
     3541 ctggattgcg gcaatcgaat tggcaactgc taaccccaaa tcgttatggc agtgaacgct
     3601 gataattgcc tctgatatgt ttggcacatt tgttttaata taacggataa ggcctccgaa
     3661 ttcatcagga acagcgtagc ctaccgtgtc cggaatattt aaggtattag ccccggcatc
     3721 aataactgct tcaaacacct tacagagata gtcccgatcg cttcgtgttg catccatggc
     3781 ggagaactct acattatcag tgtacctctt ggccctcttt actgcattaa ctgcaaggtt
     3841 gagaatttct tctttatttt ttttaaattg atgtcttaga tgaatgtcag aggttgagat
     3901 gaacgtatgg atccttggtt tctttgcaac cttgatcgcc tcccatgcac ggtcaatatc
     3961 ttcatcgttt gcccttgcta acccagcgac ttcactgttt tttatagatt ccgctattag
     4021 ccttacagcc tcaaaatccc cttcagacgc aataggaaat ccggcctcaa taatgtcaac
     4081 accaagagct tccagctgtt ttgcaaccct taacttctca ggcgtattca ttgtgttgcc
     4141 aggcgattgc tcgccatctc gcaatgttgt gtcgaatagg aaaactcgat ccgccatact
     4201 ttcctccaag taacttctaa acgttcattc ctttcctttg atcaccatga aaaataacgg
     4261 aatactaaaa aaccatattc attaatcaat gtcaatgaat ccagtatttt tgagccctga
     4321 tgccatttcc catagctggc ataggtactg ctgctgcttg aatttttttc aggataacat
     4381 caataagttc ctgttctttt tcaatgttta cctcaatgga aagataaaaa agattgtctg
     4441 gtacaggcat tccaatcatt ttcaccgcat ctttttcagt tgtaacaatc aaacttatat
     4501 caccataaga cgacagttct tcaatatcct tgtttttgta cgtgtaatga tcttcatagg
     4561 cgacctcgtg caccacatcg gcccccaatg ctctcacaag ttcaaagaat gacctgttat
     4621 caccaagccc cgaaaatgca agaactttcc tcccattgag tatgttatag tgggtactga
     4681 ggttatgttt tacgttgtag agatgcattg gtctgtatgt catcctgaac gttggaatcc
     4741 catatattac atctgcaatc tcaggctcta tgctgccatc attaacaaga atggcatcag
     4801 catcctttag cctgataatg ggttctcgac aaggaccaag aggaaaaaga ttggtggatt
     4861 tattatactg atcactcttc aggactacaa tgtcaacatt cttcaaaata ttttttacct
     4921 gatacccgtc atcgagaatc gccaaattaa tgttattctt tctcattgct tcaacaatag
     4981 ccagtgatct ccttttaccg acaaagactg ggatctttgt ttttttggca agcattagtg
     5041 cctcatctcc cacatccttt gccttatcat tatctttgtc aacacagaag atgccctttt
     5101 tttttctttt atatccacgc gttattattc caggattgaa cccaatttct ttcattctgt
     5161 aaccgagtct ttctaccaag ggggtctttc ccgtgccacc aattgtgatg ttcccaatgg
     5221 ctaaaacagg gattgggact tcatctattt ttaataaacc cgagtcgtac aatgcatttc
     5281 ttaaatgaag acaaagacca tatagttttg aaagacaaat gaatggcaac aataagaacc
     5341 atttccagta tcttgcttca cctttccaaa attttattac aatatctttc atttatagga
     5401 taatttatac cagataatgg cataaaattc aaggggatag ctacatcact gggcaatgag
     5461 gatcatgtat acttgataat agaaaataag ggaggaatga tttggttgca gtggcagatc
     5521 gggcacttgc caggctttgc cagtttatct cttttcctga aaacaaaact gcatttagaa
     5581 cactcagcag ggtaaacaac aaggtgataa ctgcccttat tcatggtctt ttttatgtgc
     5641 acaagatgat catgaacatc tttttcagga atttttaaaa aatttgaaag ctctttcgct
     5701 gaaatagtat agtcctgtaa aagggctaca atgtactgac gaatcgtctc atgtctttca
     5761 ataggggtaa tggactcttt tgactgtttt tgcatgaact attatacagc atcttgaaaa
     5821 acaggggcaa atcagacatt aataactctc tctatattaa cgatttcctc acgagcacca
     5881 ttcagactct cgatgaaatc aagaaaatcg agtccacgaa ctccaggatt tatccgttca
     5941 agtattttga aagcgtctgt ttctctaagt gttgtcccat ctatttctcc tccaaaacaa
     6001 aagcttttaa gttttgaaat atgattagcg aatgagacaa ttgacactgc aatcagacat
     6061 tcgtcaggag ccttatatgt ctggtgatgg tacatggcga cctggctaaa aataatgggg
     6121 agttgccatt tctccataag ccaggctccc accatgtcgt gagaagcgcc gatcacacgt
     6181 ttttccgcct ccaggaccga aatattctgg ccacgggcca gacgcaagac atccgtatac
     6241 atatcatgag caaacttatc aagcacaatc ttgcctacat catggataag acccgcaaga
     6301 tagatattat tcagcagtcc tatgtttagc tcgctggaaa gaagttttga aaataccccc
     6361 gttgctacag aatgcctcca gaacattatc aaatcaatcg taggttgtct tgatgacggt
     6421 ctcaatatac tgcatgcgct gcaggcaata cacagggagg taatctctct tgtcccaagc
     6481 attctcacag cgtgttctat cgttgcaacc ttatttctaa gaccataaaa ggctgagttc
     6541 gcaaccttta agacgttcat gctcattgat gggtctttgg agataatttt cccaagatca
     6601 ttgaggtcga tgtcgtcatt caaagcctgt atgagttctg ccatgaccga cgggattgga
     6661 ggcaggtatt caatagtctg cttaatcttg ctctcgaaat cttccatcat acttttgtct
     6721 cagcagcaca tttgcttcat atgtattttg tacttattat cggaaaatct cttttctact
     6781 ttaaaaatga tttttgagat tatttaccgg tgttttgaca ttgattgaaa tgagttacat
     6841 cacagctcaa actattcaat tattgatgat cctctccatt gccacaattt cattatatgc
     6901 ctcgtcaaga tcccaaatga acttgactac atctagattc tttatttgtg gataggcttc
     6961 atcaataatc ttccacgcat cgatatctgc aagtacaacg ccactcatat cgccgccaaa
     7021 cccgaagctt ttgagtcttg caagttggtt cgaaagagag actatagcca cttccagcct
     7081 actttcctcc ctggtttcca ttactgagtg gtggtagttg gctatatcca cgaaaattga
     7141 aggcagattc catgccttca tcagccaccc accaaccttt gcatgtgatt cgcctattac
     7201 ccgctgttct gcctcaatca ttgatatatt ttcattgtaa gtaatcttca caatctcttt
     7261 gtagatatca tgcataaacc tatccagaat gatcttacca acatcatgga taagacctgc
     7321 aagatatatg ttattttgtg tcagaatgcc aagttcgttg caaagcactt tggaaatcac
     7381 accggtagcc acagaatgct gccaaaacat actgagatca atggtagctt tattagccgg
     7441 tgcctttaaa gtttttcctg cagtacacgc tatacaaagc gaggcaattt ctcgcgtacc
     7501 taacatacgc actgcatgat ctatagtggt aaccttcaca ggcaatttat aaaacgctga
     7561 gttggctact tttaaaacat tgacactcat cgaagggtct tttgcgataa tttttccaag
     7621 gacattcaat tcaatatcct cattattaag cgcctgaaca agctccacca taacggtcgg
     7681 taccggtggc aaataactga tcatttttaa cacttttgac ttaaaatcct taattatcac
     7741 aaaacacctg catctattca caagatttca gacattaata tcattatcgg atattcaacg
     7801 atttgtttta agatataatt cacctgactt tctatcagta cctgcgattt ccattgcgaa
     7861 aacatagaat cgccgtgtac aataacttta cgagaaattt attgttgata caatttccat
     7921 gagtaaatac aatctcattt catgtttcgg cattcttttt tttatatgtt tccttgccct
     7981 tctttcgcga caaaaacgat ctattagtgt aaggatttta ttctggggga taggtcttca
     8041 aatccttttt gccttttttc tcttctgcat acccgttgga accaagtttt ttctcttttt
     8101 gaacgatttt gccatgggcg taattgatag ttcttttcag ggttcacaat tcctctttgg
     8161 aagacttgct attgctccag gcaaggaagg ctctattgga ttcatccttg cttttcaagc
     8221 tcttccctcg attatttttt tcgctgccct tatggaatta ctctattata ttggcgttat
     8281 ggagaagata atcgcccttt ttgccagaat ttttataaaa gttatgggca taagcggagc
     8341 cgaggcactt tgcgcctcgg ctcagatttt tcttggcatt gaatcaaacc tgactgtacg
     8401 accatatctg tcgaaaatga cacagtcaga acttctcgtt gtcttaacga ctggcatggc
     8461 taccattgca tcgagtgttt tgggactcta tgtgattata ctgtcagatt catttcccaa
     8521 agtggcagcc cacctcatat cagcgtctat cctcctggca ccagcagcca tcctcgcgag
     8581 caaactgata atccctgaga catacaagcc ccttactctg ggaaccactg taaaaattga
     8641 aagccagagg gcaggtaatg taatcgagtc aatcatcaat ggttccatgg caggtctcaa
     8701 aatggtggtg ggaattggca ctctgcttat tgcttttata ggcctgattt ctctaattga
     8761 tgtgggtctc ctctttactg gaaaactttt cagcacagtc ctcggtataa gggttgatgt
     8821 aacccttcac acaatcctcc agtatgtctt ttatccattt actgtagcaa tgggtgtgcc
     8881 acttgttgat gcccagacca tagccagaat cattggtgaa aggataatag aaacagaggt
     8941 caaatcctat caggatcttg ctttattgat caaggaaggt aagatcgtat atgaaagaag
     9001 tattgtaatt gcaacgtatg ctctgtgcgg attcgcacat ataccttccc ttgccatctt
     9061 tgtgggaggt acagcaagct tagttccgga aagaacaaag gacatatcga tacttggggt
     9121 gtgggcgctt atcgcttcca atattgcctg ctttattacc ggggctgtag caggcatctt
     9181 ctacaccagt ggtggatctc attttctgaa gtgatgttgc gctatttcat cttttaggcg
     9241 gtggaagtgc tggtttagat agttttggtt tttctggtgc ggctttcttc ttttctgttt
     9301 tctgtttctg ctgttgtgcc tttgcctcag cttctcttct ttgaacctta ctactgttta
     9361 tttcgttttt aagttgcgct atttcacttt ttagcccatc cttctcccgt atggacttct
     9421 caattttacc ttcaagctca gaaatctgcc ctttaacatt ttttaagtct gtaagttcac
     9481 ccttaaggac ggccatattt gaacccagtg atagtaccat tgcaccaagg attacaacta
     9541 caataacgag aacaatggtc cccaagggta aacttttcat aaaaggtact acactgctac
     9601 ttttttttgg tcttttcttt tcctgacttt ctaaaagttt taaaagatct tcagaatcgt
     9661 ctatcttgtt catttcttca tcaaaattgg ccatctctaa tctcctcaaa ccgtctatga
     9721 tgttttaatt accatattat ccaggtaaat tacaaaacta aaagagttct gtgggagttt
     9781 aaattatatg tatctgcttc aagaacattg acaaacgagt gaaaaaaagg gtaattttca
     9841 ataggttaca tggatagaac actccttctc aacacaacat ttgaacccct tagcgtgctt
     9901 tcgtggaaaa aggcagttac cttgattttt cttggaaaag tggaagttct gaaggaatat
     9961 gaaagggaaa ttaagggtgt ctcgcgcagc atcagacaac ctgcagtaat acgacttgta
    10021 cgccttgtgc gcaacaacca tgtaagcgtt aaattttcca gaaggaatat ctttttacga
    10081 gacgatttta cctgccagta ttgtggaaaa aggcacgatc ccaaaggcct tacctgcgat
    10141 catataattc ccaagtcaag gggcggggca acggagtgga caaacattgt tacctcctgc
    10201 attcgctgca accacaagaa gggtgacaag cttcctgagg aggtcgatat gcttccccgc
    10261 aagaggcctt cgcggccaag cggtttctat atgcttacgt tgcatcttgg tgttaagagc
    10321 cttccagatt attggaggga ctatctattt ttgagggatt aaaatggatc gaatttgggc
    10381 gccgtggaga atggaataca taactgcttc atcaacatca gataacaggt gttttctttg
    10441 cgtggattca tcgcgtgatg accaggccct cgtggttgga agaaataata ctgccttcat
    10501 tatcatgaac agattcccct atactaacgg tcatgtcatg gtcgttccga tcagacatgt
    10561 tggttccatt gatagcctga cagatgagga aacccttgac atgatgaggc tgacaaaaat
    10621 catggtagct atttttaaaa aagaatttag cgttgatggg ctgaatattg gtatgaacct
    10681 tggtagggcc gccggtgccg gccttgaaga gcattgtcac attcatattg tgccgcggtg
    10741 gtttggagat acaaacttca tgcccatctt tggagaaaca cgtgtaatat ccgaacacct
    10801 tcatgcaagc tacgaacgtt taaagagaag atttcttgag cagtcttttt gatacctcgc
    10861 caccagtaat aaaacccgct gacattgtaa ggacttttac cggtaaaagc caggaagatc
    10921 ttcaactgcc gcgaagagca atcataacat tcaatgctgg tgatgtaaaa gcactactca
    10981 caggtggttg tcagaagcct gttaaagcat gggaaaactt tcgaactctt ttcaggattg
    11041 cccaaagcaa taccattgta acacgaagct tttttggtgg ccccaacata gcagcccttg
    11101 ttgaagaact atccgccttc ggagtgcgcg agtttcttct ttggggatat tgcggcggaa
    11161 tcgctgatga tatatctata ggagatctgc tcattgcaga gggagccttg cgacaggatg
    11221 gtctgtcata tcattacctc agtgataacg atgactttgt ctattcgagt tggttcccaa
    11281 agtggcgcaa agcagcaatt gcatcacaga tcattccttc cctgatttgg agctgtgatg
    11341 ccctgtacag ggaaacaaga aaaaaaatca acctgcataa gaagatgggg atgcatgctg
    11401 tagaaatgga ggtggcctcg ttttacgcgg tttgtaaggc aaaaagactc aaagggattg
    11461 ctttcctcgt tgtctccgac ctcttaagga aagacaaatg gaaacctggt ttttctaaag
    11521 aatcattcaa aaaaggtgca aggaggctta aggaatttct cctggagcag ggaatactcc
    11581 gttaaataaa tcagtgattt gaaccacccc gcaaaagggg ctagacatac cacatttcca
    11641 tttcgaaact ctcagagggc accttcacag aaacgtatcc cctctttgga agcctgtcga
    11701 ccatcatatc cctaaccttt aatgacagcc atatgttaat cttttcatct gctttcatgt
    11761 caagggcctt tcggggacat tccagttcga ggatatcagc gtaggccagc gaaacttgaa
    11821 ttggcgcttc ctttatggca tctttgacac ggtatattat tttggttgaa gactctgtta
    11881 tgagatttat ttcaaaagac acatcaccca tgttttccac aaagttacga tcaatatcag
    11941 cacgaaggaa aaatgagttt tcattaaaac catagtagca gcctttaatg agtaaagcag
    12001 tatcatgcat tgcaacacca tggccttttc cttctataaa tcctgaccct atccaatcaa
    12061 agtatgtggt aacttttccg tcaatttttg ggtaaatgaa gtcaataggc tctcgcgttg
    12121 gcttaatctc ctgatcttca agaaggatgg gaatggccag cacctctggg ggctccttac
    12181 cggagaatcg atacacattg gcaagatttt ccctgaacag aaaatcaaag acctcatcat
    12241 tttccgatga atgatcatca ccataccacc agaaccaatc actcccctca gctatgtaga
    12301 tactttccca cgcttgttca ttttccatct cagggtcttc gttctgaaga aactctctgg
    12361 tctgtgagag cagatcccat ccagcgttat cctcgacttg cccaatccag atagaaaaat
    12421 tgtggccaat ccaagaaccg gcaaaagtgc ccgaaatcct tccataatct tccgttttat
    12481 caaaatattc actaatggtg gtgggtacta tactttcttc cttgagaatg tcatcataga
    12541 ggtatctaag gaagtcccat ccgtcatttg catagttttc ccacgcattc tctccatcca
    12601 gggcgatagt aatgattggc attctgatgc ggcccttcac tgaattcctg atttctttaa
    12661 gccttctcag gcagtcattg gcagcatctt tcgatgccat ccgtgaatag tgaaatgata
    12721 tcaggtcgga aagctgcttg tccctgtata gaaccttgag caacctccct tccctttccc
    12781 aaatataggg ccgatacaga aggtcaggat tcgtaaggtt tccgtagcca tcccgcgtgt
    12841 tgtttacccc aaggctctgg aaaagaattt cttcgtctgt aactgaccat tctacaccgt
    12901 gctcagcata tagggcaaat gaatcattac ttacacaacc ctcaggtggc cacataccgc
    12961 gcggtctatg cccaaagagt tcagagaaaa agtcaatagc tttctttatc tggtatgatg
    13021 catcttctct ctgtctaaat aacttttgag gaagaactgc atcaggtaag gcttccctgg
    13081 cagcagcact gtctatgagg agcgggataa taggatggta ataggctgac gtggaaagct
    13141 caatggagct gttttcgtaa agttccctgt ataacgggat gatacttttc aggatatttt
    13201 tctgaacatc ctgaataatt tttttatcgt cttcgctgaa tgatctacct tttgacttta
    13261 agtatttcag accatcatat tgttcaaaaa acatgggatc gatccatgcc aggaaaaaaa
    13321 agatctgaat atcacggagc tcctcatcag taaaataatc tttgatctct tttatacctt
    13381 ccttaggata gtaaaaaccc ctcttcttta ataactcaaa ataatgggga aagggtttta
    13441 tcatgttgtc ccagttggca ctgaaaaaat tcatcaaaag ataggttttt tgagattctg
    13501 tgagatccct gggcggtgtc ctgaatatct caagatacac gtcctttgcc tgtaaatctt
    13561 cgtaatccat taattgcctg agtagagatg ggacaaggtt aaaattctgc ttaagaccag
    13621 ggaattcttt taaaatataa gccatatcat aataatcctt ggttccatgg agaagtaccc
    13681 aaggaagcaa gtattcgcct gtaaatagat ttttgtaaaa aggttggtgc atgtgccaca
    13741 aaaatgcaag atgaattcta ctcattatct tagctctata agattaaatg aaatcttttc
    13801 tccaagttca agtatgccca cagttcggct gctcttatat gaaccgggat taaacaggtg
    13861 gatatttctc tttttggttg acaagggaat atgggaatga ccaaagatta cgatgtcaac
    13921 gttctcgaac ttgtccagta cgaattgttc caaccagaag ggagaccccc ttccgtgggt
    13981 aattcctatt ttccatccct ctatttcttc aactctcttt tccggaagga tagcacacaa
    14041 atcggagtcg tccatgttgc cagcgacagc tttcaaatcc cactttacca gatagtcgtg
    14101 gacaagtaga cctgtcatgt ctccagcatg aatcacgatc tcaacttcag cgaaacactt
    14161 gtccagggta tacttaaaat catcagtggg tttactgaga tgggtatcag aaatcacgcc
    14221 tattctcttc atataggtgc ttctatttct gctgcaatgc tttatcaatc tccggtgcat
    14281 tggcgccgga gataggcttt ccgttaacaa tgaagaaggg cgtactattt atacccattt
    14341 ttcttgatac atccttatgt gttttcagaa gtacatcgac ttctggtttt ttacagatct
    14401 cgtatttctt attatcgagt ttgccatgca ttgcttcctc atacgccttt gcagtgtcat
    14461 tggagcaaaa gatgtatttt actttgttct cggcatcttt gtgcattggt aaaggcagaa
    14521 agaagatata cctggttaca tctgttttct cgcttaaaaa agaagaagct ctccggcaat
    14581 atgggcaatc aggatccgtg aattcatata tagtactgtt tccattccca atcttcactg
    14641 ctttatcaag cggcagatct ctgaccttgg aaacaatgtt ttcgtttctt ctttttgttg
    14701 taatattagt accatcctta tctataatct cacccaaaat caggtatcct ggctcaggag
    14761 caaaataacc tatctgattt tgtacaataa tttcataaat tccttttatt tcggtgggat
    14821 gaattgattc aacaggtata ttggggaata acttcttaaa gctttcttct acattctgtg
    14881 gcatagccag tgacggtaag aacatgagag aaaggctcac aacaagtagg gctattaacc
    14941 tcttcagcat cctgtacgtc ctcctcaata gcgctttaga atttctatac attagactat
    15001 ttgtacttca tattcaacta aatatacatg ttcaacgatg tgactttgcc attctataaa
    15061 gacttcacgt aggctcaata ctattttcta aaaagaga
//
LOCUS       DAOOMC010000071        15018 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdus sp. isolate AMR_MDS_2685
            NODE_4228_length_15018_cov_6.505313, whole genome shotgun sequence.
ACCESSION   DAOOMC010000071 DAOOMC010000000
VERSION     DAOOMC010000071.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864760
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdus sp. (wastewater metagenome)
  ORGANISM  Syntrophorhabdus sp.
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae; Syntrophorhabdus.
REFERENCE   1  (bases 1 to 15018)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15018)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,729
            Genes (coding)                    :: 2,708
            CDSs (with protein)               :: 2,708
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 7 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15018
                     /organism="Syntrophorhabdus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4228_length_15018_cov_6.505313"
                     /isolate="AMR_MDS_2685"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2699756"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1882
                     /locus_tag="PK125_08415"
     CDS             <1..1882
                     /locus_tag="PK125_08415"
                     /inference="COORDINATES: protein motif:HMM:NF023418.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3488 and transglutaminase-like
                     domain-containing protein"
                     /protein_id="HPB38171.1"
                     /translation="SYLVGAMVFLSIVKDIGTLYSIVFCLLFAISLFFDYRRSFRIPR
                     WLLTTLSLAIVILALYRLNMEELITQMMETLLTLLAIKLLEEKKVRDYLQIYAITLLL
                     LSGSGLLSLNIAFFVFYLVIIFTLTIAAILLAYYTQDRDLVLPQGTIVKIVTKSLIIP
                     ILAIPLTFVMFVILPRTAYPLLNFLNRPDKARTGFTDQVRLGAVSDIQEDSTIVFRAN
                     MEKIDEKHLYWRGIVLEYFDGSSWIRGKKRSAPRSFPGSLGGKSVKQTIYLEPYGNGY
                     LFSLDRPLFVAGQNIKKSQDFTYSSTGYIDTRLKYEVISVLGDNIPEDAIDQTRYLQL
                     PKNLSPRIVKLAKDFSTDSHDKTIQRIIGYLQSNTFTYSLRALPSSKNPLEDFLFETK
                     SGNCEFFASALAVMLRLNNIPARLVGGYRGGYFNDVGQYYLVPQKYAHAWVEAYVKPK
                     GWVRLDPTPAVPGSAGFLSGAGGLQNFTILMDTLNYYWYVLVINYNLEKQFSIALKLR
                     SELRNLNFSPGFQRDKALKYAVIFIFFVGIAIPMYVLIKNGSKPPEEKILASFLKKMK
                     KRGYSKRASQGLEEFVQTIEDERIRESALDFTQRFHKIYFRDRIFTRNDLQDLRKIVS
                     LI"
     gene            1984..2961
                     /locus_tag="PK125_08420"
     CDS             1984..2961
                     /locus_tag="PK125_08420"
                     /inference="COORDINATES: protein motif:HMM:TIGR02122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TAXI family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HPB38172.1"
                     /translation="MRKFVFFLLVLCLPVFISTTGTAQTKKDWPTKGATIGAAPIGGV
                     YYVWAGGPAKVLGEKLGIPASVESTGGPVHNVQLVNAKELHFGMVTAAPAYEGWNGTG
                     WAKGKKYQNIRAIFPMYTTYFQMYVMKKSGIKSIKDFNGKSIGTGPVGGTPATYWPMI
                     IAEAGVKPKRIVNASSSDLDNQMKDGLLDASGQAVGLPWGLISATETTHEIVVLGVED
                     DIATSFIKKHPDFAKGIIPKGTYKSLQSDLPTLTVWNFMVTYKDMPDDFIYAFTKEIF
                     KNKPLLVSVHKSAQEVEPKNILYCPIPLHPGAIKYYEEVGIKIPKEMRP"
     gene            2999..4915
                     /locus_tag="PK125_08425"
     CDS             2999..4915
                     /locus_tag="PK125_08425"
                     /inference="COORDINATES: protein motif:HMM:TIGR02123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter permease"
                     /protein_id="HPB38173.1"
                     /translation="MEEKNIDLGKIKDADLIRTREFGKIGSWIIYGVGAICSLFHLYC
                     LLLRPIDPWYFRGMHIVFLGFLCFLLYPGWDRARKRVHPIDYVLIFMIIFPFVYMVVD
                     FDEWIYRVGVVPTTWDTIISLMFIVAILEMTRRTTGLALMIIATVFILYGYFGKFMPG
                     LFYHKGYSWPRIFTYLFSLDGILGLPIFVSAAYVYIFVIFGAFLKSSGVGNYFIQFSN
                     AIIGWARGGPAKVAIISSSLFGTVSGSSAANVVGTGSFTIPMMKSMGYKSHFAGAVEA
                     VASTGGQIMPPVMGAGAFLMAEILGIPYVKVLVSAILPAAMYYLAVYFMVDFEAAKTG
                     LVGLPREQLPSKKETFKRIYMFTPLGVLVYYIAIAMASIIKAGTLAVAACVVVSWTSK
                     ETRMGLKKIVDSLANGAKGAIEMAGTCAAAGIIIGIISQTGIGLKFATILLEFSRGIL
                     PLALFLAMVVAIILGMGMPTTAAYAICAAVITTGLTNLGAIPIAAHLFVFYFACISAI
                     TPPVALAAYAGAAIANAHPNKVGFTAMKLGVAAFIAPYMFIFSPALLFIGSLFDVIEV
                     SITALLGTFALACGMQGWLIGRAIPFLLRIPLIAGALLLIKPGIYTDIAGIGLLIATY
                     FVSRIWKKPLVSAG"
     gene            5262..6638
                     /locus_tag="PK125_08430"
     CDS             5262..6638
                     /locus_tag="PK125_08430"
                     /inference="COORDINATES: protein motif:HMM:NF014443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015379 - potassium:chloride symporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HPB38174.1"
                     /translation="MPKRVITYFRTFLLRHLTPPRTFVLSFAGVIFIGGFLLWLPFSA
                     TKDHLTLVDALFSSASAVCVTGLVVIDIGKDLSLAGQIITMMLFQCGGLGIITFSVFF
                     FHLMGRSVSFKGREIVQSTFLHTPRKDFQIILKSVLLSTLIIESFGTVLLFFRFSQDF
                     SPGQAFYHSIYNAISAFNNCGYSLFSDNLIQYQSDVIVNITIMGLIVLGGIGFIVQHE
                     VLSRMRNTRNRLSLHTKIVLATTGILVVGGAVLFYLFEKNNIIRDASWQSKVLISLFQ
                     SITPRTAGFNTVDIGQLTNDTILLLMTLMFIGASPGSTGGGIKTTSTALLFFVLWNRL
                     KGQEEVSVFNRTIPRETITRAISIILASAFSIALITSIVLLAGPKTNPSESRYLFVEY
                     LFDTISAFGTVGLTMGITPKLSDLQKILIMIMMFVGRVGPLTLAFSLSIQASKKSIVY
                     AEENIMVG"
     gene            6649..7293
                     /locus_tag="PK125_08435"
     CDS             6649..7293
                     /locus_tag="PK125_08435"
                     /inference="COORDINATES: protein motif:HMM:NF014325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HPB38175.1"
                     /translation="MPRVVVIGLGIFGYNVVKNLFEGGMDVIAIDKNKDAVQKVRDYS
                     SRAIVADGIDKSVMDSIGIGADDVVILSFGEDLAASTLATLHLKQLGVRNIIVKAPNE
                     EHKLVLEKVGATEVIIPEKEMANKLARGFISPNLIDYIPLSDDYMICETAPPKQFIGK
                     SLRDVHMRSKHHVDVIAIRDTLTDNVHMVQPDYLMKDSDILVVIGKSDDIDKLK"
     gene            complement(7368..8687)
                     /locus_tag="PK125_08440"
     CDS             complement(7368..8687)
                     /locus_tag="PK125_08440"
                     /inference="COORDINATES: protein motif:HMM:NF028290.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HPB38176.1"
                     /translation="MFARYTFLFIIMAFAPSPAFAGTHEVSFPLWTTGFFALLILSIA
                     FMPLINGEWWSKHYGYVSLGLALPTIWIVGSHDFALILETLSEYLSFIILLGSLFVIA
                     GGIVIRTSARGGPVLNSLILGVGSIFASIIGTTGSSMVLIRPLIRANKWRRHVVHIFI
                     FFIFLVSNIGGLLTPLGDPPLFLGFLKGVPFSWTLRLISFWLFAVPILIGIFYILDRH
                     FLRKDLREMDSDIPNRIAARKLEIRGKINFFFLAAVLGAFFLPPVAREIVMIGAAISS
                     IYLTPVVLREENAFTYHPIIEVAVLFAGIFITMIPAIKILEMNGGKLGVDTAWKFFWM
                     SGLLSSFLDNAPTYLVFMSTAKSIALMQHITTGLVAGVPEAFLKAISVGSVFMGANTY
                     VGNGPNFMVKAICEENDISMPSFFGYIGWSSLFLIPIFILITLIFFL"
     gene            complement(8701..9537)
                     /locus_tag="PK125_08445"
     CDS             complement(8701..9537)
                     /locus_tag="PK125_08445"
                     /inference="COORDINATES: protein motif:HMM:NF024587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidylate kinase-like family protein"
                     /protein_id="HPB38177.1"
                     /translation="MRVITISRSYGSAGSLLGRKLAERLGYQYVDERSISRMKTNKEA
                     ASVLMMNLEDERSPGFMDRVAELMHNRSYFRTALGVAIYDLILRKDVVVVGGGGHLIL
                     RGYPLKVSIQIYRNLSDRVHDISIEKNISQDQALDLVESKDREKRRFISYYFDEDLFD
                     PISFDITLNASHVSLDDALDLIEGYCKRYFDRVNTEEAELFAEARLLEKRAQLAVFHM
                     NLMRNSKIVFKVSEPRILAVKGVVGGAGEKERLLRGLRSLKGVDKVIDQLKTGVLSRM
                     LY"
     gene            10047..12029
                     /locus_tag="PK125_08450"
     CDS             10047..12029
                     /locus_tag="PK125_08450"
                     /inference="COORDINATES: protein motif:HMM:NF024910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HPB38178.1"
                     /translation="MNHHINEQKPNIFHKIRRSLIGAPKDVRQPSIFHKLSLIPILAW
                     IGLGADGLSSSSYGPDEAFRTLGEHTYLAIFLAIGTALTVLIISYAYSRIIEHFPHGG
                     GGYIVSTHTISKTAGVISGSALLVDYMLTIAVSLVSCGDAIFSFLPLYLQKYKLMFVA
                     FLIILLVIFNLRGVKESVSMLAPIFIIFVVTHALLIGYGILTHTGQIKPLVHEVSSNL
                     KNGVATLGFLGVLGIFLRAFSMGAGTYTGIEAVSNGMQIMREPRVHTGKRTMVYLASS
                     LAITAGGLLVCYLLFRISPADGKTLNAILAGEVFSNWPMGGTIALITILSEGALLVVG
                     AQTGFIDGPRVMSNMAIDSWLPRRFASLSERLTMQNGVILMGVAAIILLLYTGGSITA
                     LVIMYSINVFVTFSLSQYGMIRFFQKNRERDTKWKQHIIIHIIGLILCLTILCVTIYE
                     KFGEGGWVTLVITSAVIGLCYLIRGHYTKVRNSVKHLEETLSDIPAANPFNTDPVDPK
                     EMTAILLVSRYNGFGLHTLLSIVKNFPGVYKNFVFVSVAEVDSGTFKGIAGLDALQAS
                     VKNDLERYVKITRAHGFPADYRTDVATDVVDHVTELVEKTVKEFPRSTVFTGRLVFKH
                     ENPFQKILHNETAYSIQRRLQWDGIPTVILPIRVDV"
     gene            complement(12068..14881)
                     /locus_tag="PK125_08455"
     CDS             complement(12068..14881)
                     /locus_tag="PK125_08455"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HPB38179.1"
                     /translation="MDTRACHFIPIEHVSSQLETRIEKGLTQVEAEKRLRNYGPNELG
                     EKARPGFFKMLLDQFNNFLVIILIIAATVSLLLGEVIDAVAIIVIVVLNAVVGVVQES
                     KAEAALAALKKMAAPNAQVLRDGHLISIPSRELVPGDIVLLEAGNYVPADMRLVESVN
                     LKIEEASLTGESVPVDKDATVVLDKEIPLGDRHNTAFMSTVVTYGRGKGLVTGTGMNT
                     QIGVIAEMLQSYEEEATPLQEKLDHLGKLLGFACLSICGIIFIYGLLRDTHMGTIFKD
                     GLLAYLSHEKGDIIKLFMIAVSLAIAAVPEGLPAVVTICLALGMQKMIRRNALIRKLP
                     AVETLGCATVICSDKTGTLTQNEMTVTDGWIKGKHFKVSGEGYTPAGRFSLNGQVVNP
                     LDDPGLSLLLHGGVLCNDAMLEETKEESSGQSWRMVGDPTEGAMVVAGAKAGLWRKDL
                     EEMFPRVMEIPFDSDRKRMTTIHEIKKSSVQGRKLSFDVPPVMVFVKGAPDVVLKISD
                     SIIENGKPVSLTDEILKEVLAENQDLGQQALRVLGVAYKPMDDLPDRCDSESVEKGLT
                     FVGLMGMIDPARPEVKAAVKIAQGAGLKSVMVTGDYKDTAEAIAREIGLRTPDGLVLS
                     GSELDKMSEGELVRVIERVDVCCRVSPQHKTKIVSAFKAKDHVVAMTGDGVNDAPALK
                     LANIGIAMGITGTDVTKETADMVLTDDNYASIVSAIEEGRIIYSNIRKFVYFLLVCNI
                     GEILIIFFSMLLGMPIPLLPVQLLWLNLVTDGAPALALGLEKGEPDIMKRPPRPTKEP
                     VINREMLTGLIVVPIVDTIAVLSVFAIGLAQYPNHVENAQTMAFMTLCVSELFRAYTA
                     RSEHYSVFKIGVFSNRSMQWAVASSLFLVLITVYVPFLRPFFDTAPLSLDDWLTMIPF
                     TLAAPVAAEIVKIFLRRGKAAHR"
BASE COUNT         4126 a         3569 c         3156 g         4167 t
ORIGIN      
        1 aagctacctc gtaggtgcca tggtctttct ttctattgta aaggacattg gcactcttta
       61 ttcaattgta ttctgccttc tttttgctat ctctctcttt ttcgattaca ggaggagttt
      121 tcgtatcccg cgatggttgt taacaaccct gtcccttgca atcgttattc ttgcgcttta
      181 caggttgaac atggaggaac ttatcacgca gatgatggaa acccttctta cactccttgc
      241 catcaaactg ctcgaagaga aaaaagtgag agactatttg cagatttatg ccataaccct
      301 tcttctcctt tcaggctctg gccttctctc tctcaacata gcattctttg tcttttatct
      361 cgttattatt tttactctta ccatagccgc cattcttctc gcctattaca ctcaggacag
      421 ggacctggtc ctgccacagg gcacgattgt caagatagtt acaaaatccc tgattattcc
      481 catcctcgcc atacccctga cctttgtaat gtttgtcata ctcccgagaa cagcataccc
      541 gttgcttaat ttcctcaaca gaccggataa ggcacgtacc ggcttcacag accaggtaag
      601 actgggtgct gtatcagata ttcaggagga ttcaaccatt gttttcaggg caaacatgga
      661 aaaaatcgat gagaaacatc tttattggcg aggcatcgtt ctagaatatt ttgacggttc
      721 gagctggata aggggcaaaa aacgatcagc gccacgatca tttcccggca gcctcggcgg
      781 taagagcgta aaacagacca tttaccttga gccatacggg aatggctatc tattctccct
      841 tgacagacct ttatttgttg ctggacagaa cattaaaaag tcacaggatt tcacgtattc
      901 ttccaccgga tatatagata ccagactgaa atacgaggtt atatcggttc tgggcgacaa
      961 cataccggaa gatgctatcg accaaaccag gtatctccag ttaccaaaaa atctttcacc
     1021 aagaatagta aaattggcaa aagatttttc aacggatagc cacgacaaaa cgatacagag
     1081 aatcatcggt tatctccaga gcaatacctt cacctattcc ctgcgtgcgc tgccttcatc
     1141 aaaaaaccct ctggaagact tcctcttcga gacaaaatcc ggaaactgtg aattcttcgc
     1201 ttcagcattg gcggtcatgt tgcggcttaa caacatccct gcaagactgg tgggtggcta
     1261 ccgcggaggt tacttcaatg atgtggggca atattattta gtaccgcaga aatatgccca
     1321 tgcctgggtc gaagcctatg ttaaaccaaa aggatgggtt cggcttgatc cgacgcctgc
     1381 agttcccggc agtgccggtt ttctctcagg ggccggaggt cttcagaact ttaccatcct
     1441 catggacacg ttgaactatt actggtacgt ccttgtcatc aactacaacc tggaaaaaca
     1501 attttccatt gcactgaaac tgcgctctga gctgaggaac ttaaatttta gcccagggtt
     1561 tcagcgtgat aaggctttaa aatatgccgt aatcttcatc ttttttgtgg gaatagcaat
     1621 accaatgtac gttttgataa aaaatggatc aaaacccccg gaggagaaaa tactggcttc
     1681 attcctgaaa aagatgaaaa aacgcggcta tagcaagaga gcttctcagg gccttgagga
     1741 gttcgtgcaa acgatagaag atgaaaggat tagagagtct gcgttggact ttacacagcg
     1801 tttccacaaa atatatttcc gggacaggat tttcacgaga aatgacctcc aagacctcag
     1861 gaaaatagtc tcccttatct gattaaactc caaatagatt tttgtaagta tttaaaattt
     1921 ttatattgac attctttttc gtaaaagtgt aatagaaaag aaaataatag ggggaggtgc
     1981 tttatgagaa agtttgtgtt ctttttactg gttttgtgtc tacctgtatt cataagcacg
     2041 accgggactg cacagacaaa aaaagactgg cccaccaaag gtgctaccat aggagcagcc
     2101 cctattggag gcgtctacta tgtttgggca ggaggcccgg caaaagtact tggtgaaaag
     2161 ctcggaattc cagcgtctgt tgaatcaacc ggtggcccag ttcacaatgt acaactcgtc
     2221 aatgccaaag aattgcattt tggtatggtt actgctgcac cggcctatga gggatggaat
     2281 ggaacgggat gggcaaaagg gaaaaaatat cagaacataa gggctatttt ccccatgtat
     2341 accacctact tccagatgta cgtcatgaaa aaaagcggta tcaaaagcat caaagatttt
     2401 aacggaaaaa gcattggtac cggtccggtc ggtggcacac cagctactta ttggccaatg
     2461 attattgctg aggcaggcgt caagccaaaa cggatagtaa atgcttcatc atcagacctt
     2521 gacaatcaga tgaaggacgg ccttctcgat gcgagtggac aagctgtcgg tttgccatgg
     2581 ggcctcatat ctgccacaga aacaactcat gaaatcgtag ttctcggcgt tgaagacgat
     2641 atagctacca gctttataaa gaaacatccc gattttgcaa aaggcattat accaaaggga
     2701 acctacaaaa gcctgcaaag cgatcttccc acgctcaccg tatggaattt catggtcaca
     2761 tataaggaca tgcctgatga cttcatctat gcgtttacaa aagaaatttt caaaaacaaa
     2821 cccctccttg tgagtgtcca caagtcagcc caggaggtgg agccaaaaaa cattctttac
     2881 tgtcccatac ctttacatcc cggtgctatc aagtattatg aggaggtagg cataaagata
     2941 cccaaagaaa tgagacctta aaaactaaaa acatttaata gatacaatgg agggggggat
     3001 ggaagagaag aacattgatc ttggcaagat caaagatgca gatctcatac gtaccaggga
     3061 attcggcaaa atcggatcct ggataatcta cggggttggt gcaatctgtt ccctcttcca
     3121 tctctattgc cttcttctga gacccatcga cccctggtat ttcaggggaa tgcatatcgt
     3181 attcttggga ttcctctgtt ttcttctcta tcctggatgg gacagggcca gaaagagggt
     3241 tcaccctatc gattatgtcc tcatctttat gatcattttt ccttttgttt atatggtggt
     3301 cgactttgat gagtggattt atagggtggg agttgtccca accacatggg acacaataat
     3361 ctcgctcatg tttatcgtgg ccattctgga gatgacccgg agaacaaccg gtctggcgct
     3421 gatgatcatc gccaccgtat ttatccttta cggatatttc gggaaattta tgccgggtct
     3481 tttctatcac aaaggttaca gttggccaag gatcttcacc tatctcttca gccttgatgg
     3541 gattctcggc ctccctatct tcgtgtctgc tgcttatgtc tatatctttg taatctttgg
     3601 tgccttcctc aaaagctcag gagttggaaa ttatttcata caattttcta atgctataat
     3661 cggttgggcc cgtggtggcc ctgcaaaagt agctattatc tcgagctctc tcttcggcac
     3721 tgtttcaggt agttctgcag caaacgttgt aggcacagga agcttcacca tccccatgat
     3781 gaaaagtatg ggctacaaat ctcacttcgc aggcgctgta gaggctgtag catccaccgg
     3841 tggccagatt atgccccctg ttatgggagc aggcgccttt ctcatggcgg aaattttggg
     3901 aataccttat gtgaaggtac tggtatctgc catacttcct gcagctatgt attatctcgc
     3961 cgtatatttc atggtagact tcgaggccgc aaaaacaggc cttgtagggc ttccaagaga
     4021 acaacttccc agtaaaaaag agactttcaa gcgtatatac atgttcactc ctctgggcgt
     4081 actcgtctat tatattgcta ttgccatggc atcaatcata aaagcgggca cattggccgt
     4141 tgctgcatgt gttgtggtaa gttggacgag taaagaaacc cgcatggggc tgaagaaaat
     4201 agtcgactct cttgcaaatg gagcaaaggg agccattgag atggcgggaa cctgcgcagc
     4261 agccggtatt ataataggta ttatctctca gactggcata gggttaaagt ttgccacaat
     4321 cctcctggaa ttttctcggg gtatccttcc ccttgcgctg ttccttgcca tggtagttgc
     4381 tattattctc ggtatgggca tgccaactac agccgcatac gctatctgtg cagccgtaat
     4441 aacaacaggc ttgacgaatc ttggggccat accgattgcc gcgcacctgt ttgtcttcta
     4501 ttttgcctgt atttcagcta ttactccgcc agtagcactt gctgcctatg ctggggcagc
     4561 cattgcgaat gcccatccaa ataaggtcgg cttcactgct atgaaactcg gcgttgcagc
     4621 ttttatcgca ccgtatatgt tcattttcag ccctgcctta ctcttcattg gcagcttgtt
     4681 cgatgtaatc gaggtttcca ttacagcctt gctcggcact tttgcattag catgtggtat
     4741 gcaaggatgg cttatcggca gggcaatccc ctttcttctc aggattcccc ttattgccgg
     4801 tgctcttttg ctcattaagc cggggattta tactgacata gccggcatcg gacttcttat
     4861 agcgacttat tttgtatcga ggatatggaa aaaaccccta gtttctgcag ggtaaccaat
     4921 atttatgcaa acccggattt aaacacgccc cttgctgaaa aaattaacat acacggccct
     4981 aattacaaaa aattgtctca ctatactctt ccttttttag aatattttta aggatggcat
     5041 ctaaacagcc ctgtctaatg aaaacctcag ttgaggtaaa caattcgcac ccgcctatga
     5101 atatttttaa gaattcttta tcaatagaaa attttacaag tatttcgcgg actccataaa
     5161 ccgctgagcc ctaaaatccc tcatgatcta ttattttatt gactacagaa aatgattgga
     5221 atataaatca gtcgtcggag gaaaaagacg tcgaataaaa tatgcccaaa cgagtgatta
     5281 cttactttcg caccttcctt cttcgtcatc ttaccccgcc aagaacattc gttctaagtt
     5341 ttgctggcgt catcttcatt ggtggttttc ttctctggct acctttttca gctacaaaag
     5401 atcatcttac ccttgtggat gccctcttct cctctgcctc agctgtttgt gttacaggtc
     5461 ttgtggttat tgatattgga aaagacctgt cacttgctgg tcaaattatc actatgatgc
     5521 tgtttcaatg cggtggcctt ggtatcatta ctttctctgt ctttttcttc caccttatgg
     5581 gccgcagcgt atcattcaaa ggcagggaga ttgttcagtc cacctttctc cacacaccaa
     5641 gaaaagattt tcagattatt ctcaaatccg ttcttctttc cacccttatt atagaatcat
     5701 ttggcacagt gcttctgttt tttaggtttt ctcaggactt ttcccctgga caggccttct
     5761 atcacagcat ctacaacgca atctcagcgt tcaacaactg tggctattcc ctgttctctg
     5821 ataatctcat tcaataccag agcgatgtta tagtaaacat cactatcatg gggttaattg
     5881 tccttggcgg aattggattc attgttcaac atgaagtgct gtccagaatg aggaacacca
     5941 ggaacagact ttccctgcac acaaaaatag tgcttgccac aacaggcatc ctcgttgtag
     6001 ggggagctgt cttattctat ctgttcgaga aaaacaatat cattcgcgat gcgtcctggc
     6061 agtcaaaggt gcttatttct ttgttccagt caatcacacc gagaacagcg ggattcaaca
     6121 ccgttgatat aggacagctt acaaatgata ccatcctttt gttgatgacc ctcatgttta
     6181 tcggggcatc accaggttct accggcggcg gtattaaaac aaccagtaca gcacttctct
     6241 tctttgtcct ttggaataga ttgaagggtc aggaggaggt aagtgtattt aacagaacga
     6301 ttccccggga gaccataacc cgtgctattt ccattatcct cgcctcagcc ttttcaattg
     6361 ctcttatcac atcaattgta cttcttgcag gaccaaagac aaacccttca gaaagtcgct
     6421 atctttttgt ggaatatctt tttgacacaa tctcagcgtt tggcactgtg ggtttaacca
     6481 tggggataac cccaaaatta agtgatttgc aaaaaattct gattatgatc atgatgtttg
     6541 tcggaagggt gggtccactg acacttgctt tttctttgag tatccaggct tcaaagaaga
     6601 gtatcgttta cgctgaagaa aacattatgg tcggataggg aggctcatat gccgcgagta
     6661 gttgtaattg ggctgggtat ttttggctac aacgttgtaa aaaacctgtt tgaaggaggt
     6721 atggatgtca tcgccatcga taaaaacaag gatgctgtcc aaaaggtgag ggattattcc
     6781 agcagggcaa tagttgcaga tggaatagat aagtctgtta tggactccat tggcatcggt
     6841 gcggatgatg tcgttattct ctccttcggt gaggatttag cggcaagtac cctggcaacg
     6901 ttacacttaa aacagcttgg tgtacggaat atcattgtca aggctcctaa cgaggagcac
     6961 aaacttgtgc tggaaaaagt tggcgccact gaagttatca tccctgaaaa agagatggca
     7021 aataagttgg caagaggctt catctccccc aacttaatcg actacatccc gctttctgat
     7081 gactacatga tctgtgaaac agctccacca aaacaattca ttggcaaatc actcagggat
     7141 gtccatatga ggagtaagca ccatgttgat gttattgcca ttcgggatac actcacagac
     7201 aatgttcata tggtacagcc agattatcta atgaaagata gcgatatcct cgtggtaata
     7261 gggaagagcg atgacattga taagctgaaa tagccgcctt atcaaaggcc ttctctcatc
     7321 gctttgttga gattgcagtc cttcgataca gccgctgccc cacaactcta tagaaaaaat
     7381 atgagagtaa tgagaataaa tataggaata aggaataagg aagaccaccc aatatagcca
     7441 aaaaaagacg gcattgatat atcgttctct tcacatatcg ccttgaccat aaagtttggg
     7501 ccattcccca cgtaggtgtt cgctcccata aaaacagaac ctacggaaat ggctttcagg
     7561 aatgcctcgg gcacaccggc aacaagaccg gtggttatgt gctgcataag ggctatactt
     7621 tttgcagtac tcatgaatac cagataggta ggggcattat caaggaagct tgagagcagt
     7681 cccgacatcc agaagaactt ccacgcagta tccactccaa gctttccacc attcatctcc
     7741 aggatcttaa tggcaggaat catggtaata aagatgccag caaatagaac tgccacctca
     7801 atgatagggt gatatgtgaa ggcattctct tcccgtaaga ccacgggggt caagtatatg
     7861 gaagaaattg ccgctccgat cataacgatc tcccttgcca caggaggtaa aaagaaagcc
     7921 cccagcactg ctgccaggaa gaaaaagtta atctttccac gaatttcgag ttttcgtgca
     7981 gctattctat ttggaatgtc cgagtccatc tcccgcaggt ctttcctgag aaaatggcgg
     8041 tcaagaatgt agaaaattcc aatcaggata ggtactgcaa acaaccagaa cgagataagc
     8101 ctcagagtcc acgagaacgg aactcccttt aagaaaccta aaaataaagg ggggtcaccg
     8161 agcggggtaa gaagcccgcc gatattagat acgagaaata taaaaaaaat gaaaatgtga
     8221 acaacgtgcc tgcgccattt gttcgccctg atgagtggtc gtatcaatac catgctggac
     8281 cctgtggtac ctataatgct tgcaaaaata cttcccacgc caagaattag ggaatttaaa
     8341 acaggcccgc ctcttgcaga tgtacgtatc acgatacccc cggcaatgac aaaaagggat
     8401 cccagtaaga taataaaaga cagatactca ctcagtgttt cgagaataag ggcgaaatca
     8461 tgggagccaa caatccagat ggtaggaagg gcaagcccga gcgaaacata cccataatgc
     8521 ttgctccacc attcaccatt gataaggggc ataaacgcaa tcgataaaat tagtaaggca
     8581 aagaaacctg tagtccaaag gggaaaggat acttcatgag tcccggcaaa agcaggagaa
     8641 ggcgcaaagg ccattataat gaaaagaaat gtgtaccttg caaacattat actgcctcat
     8701 ctaataaagc atccgggaaa gtacaccggt ttttaattga tcaatcacct tgtctactcc
     8761 tttcagactc cgaagtcccc tcaacaaccg ctctttctct cctgctccac ccacgacacc
     8821 cttgaccgcg agaatgcgcg gttctgaaac cttgaagaca atcttgctat ttcgcatcag
     8881 attcatgtga aatacagcca gttgcgctct tttttcaagc agcctcgctt cggcaaatag
     8941 ctctgcctct tcggtgttaa ccctgtcaaa ataacgcttg caatatcctt ctatcaagtc
     9001 cagggcatca tcaagactaa catggcttgc attcagggta atatcaaagg atatgggatc
     9061 aaaaaggtcc tcatcaaaat aataactgat aaacctcctt ttttccctgt ccttgctttc
     9121 caccagatca agggcttggt cctggctgat atttttctcg atagagatat cgtgtaccct
     9181 gtcagaaagg tttcgataga tctgaatgct gactttcaga ggatatcccc gtaaaatcag
     9241 gtggcctcct cccccgacaa caacaacgtc ttttcttaga attaaatcat agatggcaac
     9301 accaagggca gttctgaaat aacttctgtt atgcatcagc tctgcaactc tatccatgaa
     9361 accaggcgac ctttcatctt cgagattcat cataagaaca gaagcagctt ctttattcgt
     9421 cttcatacgg gaaatggacc gttcgtcaac atactggtac ccgagcctct cggccagctt
     9481 tcttcccagc aggctccctg ccgaaccata ggaccttgaa attgtaataa cccgcattaa
     9541 ccgctccttt tgctaaaaac aattatggtc tttcatttta atgaaccggg ggtcactctc
     9601 gtaaaaccga ctttatttgt tgcgtgcaca caacgacatg agctgccggt tgcttgctta
     9661 ttttactctt ttccggcacg tctgtccaaa ataactttcg gaggacaact cgaaagagtt
     9721 taaatttgaa tcaaaccaga accgagcagg ctcttgttgt gtctatgaag cagtttagca
     9781 gcatattcgg tcagattcta cagatatttt taaaagtcga atttttcact gttgagaatt
     9841 tgcaacgatc atgcagacta tcccggaagc tgaagtaact tttccctgtt tcaagattgt
     9901 tgtatcgcgc tgattctaca cgcgttgttg catttcacca gtcatggagt taatataacc
     9961 tcaatgtcag agcgcctcag actgcacacc cgactaagta acttcaaaaa aaccaccttc
    10021 gaaaaaccga atcctcggga aacaatatga accaccacat aaacgagcaa aagccaaaca
    10081 tcttccataa gattcgaaga agcctcattg gtgctcccaa agacgttaga cagccatcaa
    10141 tctttcataa attatcgctt atacctatcc ttgcatggat cggtctcgga gctgacggtc
    10201 tctcttcctc atcctacggc ccagacgaag ctttccgcac gctgggggaa catacctacc
    10261 ttgccatctt tttagcaatt ggaacagctc taacggtcct tattatttca tatgcctact
    10321 cccggattat tgaacacttc ccccacggag gtggcggata tattgtttca acgcatacta
    10381 tcagcaaaac agctggtgta atctcaggtt ctgcccttct tgttgactac atgttaacca
    10441 ttgccgtctc tcttgtttcg tgtggggacg ccatatttag tttcctgccc ctctatttac
    10501 aaaaatacaa actcatgttt gttgcgtttt taattatcct tctcgtcatc tttaatctca
    10561 ggggtgttaa ggaatcagtc agtatgctgg caccaatctt tattattttt gttgtcaccc
    10621 atgccctttt gattggctac ggcatactca cccatactgg tcagatcaaa ccccttgttc
    10681 atgaggtctc ttcgaatctc aagaatggtg tagcaaccct tggtttcctc ggggttcttg
    10741 gtatctttct tcgtgccttc tcaatgggcg ccggtacata tactggtatt gaagctgtat
    10801 cgaacggaat gcaaatcatg cgtgaaccaa gagtacacac aggaaaacga accatggtct
    10861 accttgcgtc ttccttggct ataactgcag gcgggcttct tgtatgctat cttcttttta
    10921 gaatcagtcc tgccgatggg aaaacactca atgccatact ggctggagag gtcttctcta
    10981 attggcctat gggtgggaca atagccctta ttaccattct ctcagaggga gcgcttcttg
    11041 ttgtcggtgc gcagacaggc ttcatcgacg gaccaagggt gatgtctaac atggccatag
    11101 actcatggct cccccgtcga ttcgcttcac tttctgagcg ccttaccatg caaaacggcg
    11161 tcattctcat gggagttgct gccattatcc ttctcctata tactggagga tccattacag
    11221 ccctggtgat aatgtattcc atcaatgttt ttgtgacatt ctccctttca caatatggca
    11281 tgattcgttt ctttcagaaa aacagggaaa gagatacgaa atggaaacag catattatca
    11341 tccacattat cggccttatc ctttgcctca caatcctctg tgtgactatc tatgaaaaat
    11401 tcggcgaagg aggatgggta actttggtca ttacctctgc tgtcataggt ttgtgctacc
    11461 tcatccgcgg acattacaca aaggtcagaa acagcgtgaa gcacctggag gagacccttt
    11521 cggatattcc agctgccaat ccgttcaata ccgaccctgt cgatccaaaa gagatgacag
    11581 caatactcct tgtgagccgt tataatggtt tcgggcttca tacactgctt tctatcgtga
    11641 aaaattttcc tggtgtctat aaaaactttg tttttgtttc tgttgcagaa gtcgattcag
    11701 ggacattcaa gggcattgca gggctagacg cattacaggc gtcagtaaaa aatgatcttg
    11761 aaagatacgt gaagatcacc agggcccatg ggtttcctgc cgattaccgc actgatgtag
    11821 ccacagatgt cgttgatcat gtaactgaac ttgttgaaaa aactgtaaaa gaattccccc
    11881 gttcaacagt tttcacagga agacttgtct tcaaacatga aaaccctttt caaaaaattc
    11941 tccacaacga aacagcctat tcgatacaga ggcgccttca gtgggacggc atccctacag
    12001 tcattctccc catcagggta gatgtataaa atcttatata tggcagagtt gctttaagct
    12061 ctcgccttca cctgtgtgct gccttgcctc tcctcaaaaa gatcttcaca atttcagcag
    12121 ccacaggagc tgcaagggta aatgggatca ttgttaacca atcatcaagg ctcaatgggg
    12181 ctgtatcaaa aaaaggtctg agaaagggca cataaaccgt tatgaggaca aggaagagtg
    12241 agctggcaac agcccattgc attgaccggt tagagaaaac tccaatcttg aatacgctgt
    12301 aatgctcgga ccgggcagta tacgctctga acaattctga cacacagaga gtcataaagg
    12361 ccatggtctg ggcattctca acgtgattgg ggtattgagc aagaccaatg gcaaaaacgc
    12421 ttaatacagc aatggtatct acgataggca ctacaatgag tcctgttagc atctccctgt
    12481 tgataaccgg ttcttttgtt ggccgtggtg gccgcttcat gatatcaggc tcgccttttt
    12541 caagaccgag ggcaagagca ggcgctccgt cagtgacaag gttcagccac aagagctgga
    12601 cgggcagcaa ggggatcggc atgccaagca gcatggaaaa gaagattatc aggatctcac
    12661 ctatgttgca gacaaggaga aaataaacaa atttccggat atttgagtaa atgatccttc
    12721 cctcttctat ggcagaaacg atacttgcat agttgtcatc agtaaggacc atgtcagccg
    12781 tttcttttgt tacatccgtc ccggtaatac ccatggcaat accgatattg gccagtttca
    12841 atgcaggagc atcatttaca ccatcacctg tcatggcaac tacatgatct tttgccttaa
    12901 atgcactgac aatttttgtc ttgtgttgtg gtgagactcg gcagcataca tcaacccttt
    12961 cgatcaccct gacaagttct ccttcgctca ttttatcaag ttcactacct gagagaacaa
    13021 gtccatcagg agtacgcagg ccaatctctc tggcaatagc ttcagcagtg tctttatagt
    13081 cgccggtaac cataacactc ttgaggcctg cgccctgggc aatcttgacc gcagctttta
    13141 cctctggacg agcaggatcg atcatcccca taagcccgac aaaggttagt cccttctcta
    13201 cactttccga atcacaccta tcggggagat catccatggg tttgtatgca acaccgagaa
    13261 ccctgagcgc ctgttggccg agatcttgat tctctgctaa aacctctttg agtatttcat
    13321 cggtaagact caccggcttg ccattctcaa taatcgaatc actgattttc agcaccacgt
    13381 caggagcccc tttgacaaaa accatgacag gagggacgtc gaatgacagc ttccttccct
    13441 gaacgctgct cttcttaatc tcatggattg tagtcattct cttccgatca gaatcgaaag
    13501 gtatttccat gactctcgga aacatttctt ccaaatcttt acgccataaa cccgctttgg
    13561 cacctgcaac aaccatcgct ccctctgtgg gatcacctac catacgccat gactggccac
    13621 tactttcctc tttcgtttcc tcaagcattg catcattaca gagaacaccc ccatgcaaaa
    13681 gaagtgataa accagggtca tcaagggggt ttacaacctg gccgttcaac gaaaacctgc
    13741 ctgccggggt atatccctca ccagacactt tgaaatgttt cccctttatc cagccatctg
    13801 ttactgtcat ttcgttctgg gtaagggttc ctgttttatc cgagcaaatc acagtggcac
    13861 acccgagggt ttccactgca ggcagttttc ggatcaaggc atttcgccgt atcatcttct
    13921 gcattccaag ggcaagacag atggtaacaa ctgcaggcaa gccctcagga acagcagcaa
    13981 tggcaaggct caccgcaatc ataaagagct tgataatatc acctttttcg tgactcaaat
    14041 aggcaaggag accatctttg aagattgttc ccatgtgggt atcacgaaga agcccatata
    14101 taaaaattat tccgcaaatg gaaagacagg cgaaaccaag gagttttcca agatggtcaa
    14161 gtttctcctg taaaggtgtg gcttcttcct cataggactg cagcatctca gcaataacac
    14221 ctatctgtgt gttcatccca gttccagtaa cgaggccttt tccccttccg tacgttacca
    14281 cagtactcat gaaagcagta ttgtgacgat caccgagagg gatttcttta tcaagaacca
    14341 cggtggcatc cttatctaca ggcactgatt ctcctgttag agatgcctct tctattttga
    14401 gatttacgct ttccacaagg cgcatatccg caggcacata attgccggcc tcaaggagca
    14461 caatatcgcc tggaaccagt tctcttgaag ggatcgaaat caggtgccca tcccgcaaca
    14521 cctgggcatt gggggcagcc atcttcttga gagctgcaag agcggcttca gccttggatt
    14581 cctggacaac cccaaccact gcattgagca caacaattac tatgattgct accgcatcga
    14641 taacctcccc aaggagtaag gacacggttg cggctatgat caggattatg acaagaaaat
    14701 tattaaactg atcgaggagc attttgaaga acccgggacg ggctttttcg cccagctcat
    14761 ttggtccata atttctcagc cgtttttcag cctccacctg cgttagacct ttttcaatac
    14821 gcgtctcaag ctggctggat acatgctcga tcggtataaa atggcatgca cgagtatcca
    14881 ttaacccctc catcttttgc tacagggatt cattcaaaga atgtttgtgc tttttgtcgg
    14941 ataatgcagt tttttcgcat cgatcaaata ataccacggt aattgcgcta ttcatagaga
    15001 taattattac aatacaga
//
LOCUS       DAOOMC010000072        14953 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdus sp. isolate AMR_MDS_2685
            NODE_4255_length_14953_cov_4.758290, whole genome shotgun sequence.
ACCESSION   DAOOMC010000072 DAOOMC010000000
VERSION     DAOOMC010000072.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864760
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdus sp. (wastewater metagenome)
  ORGANISM  Syntrophorhabdus sp.
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae; Syntrophorhabdus.
REFERENCE   1  (bases 1 to 14953)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14953)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,729
            Genes (coding)                    :: 2,708
            CDSs (with protein)               :: 2,708
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 7 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14953
                     /organism="Syntrophorhabdus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4255_length_14953_cov_4.758290"
                     /isolate="AMR_MDS_2685"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2699756"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            173..4330
                     /locus_tag="PK125_08460"
     CDS             173..4330
                     /locus_tag="PK125_08460"
                     /inference="COORDINATES: protein motif:HMM:TIGR00241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008047 - enzyme activator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydratase activase"
                     /protein_id="HPB38180.1"
                     /translation="MNSIGIDIGSVSISLVVMGETGDILKSLYVRHKGRPIEVTESAL
                     ENLLKEFEIDSVAVTGTGAKEISLLIGASFINEIVAISKGFTHLYPHVRSIIDIGGED
                     SKLIILEEDRTNKKLRIKDFSMNALCAAGTGSFLDQQASRLCFTIEEFSEIALKSVNP
                     PRIAGRCTVFAKSDMIHLQQIATPDYEIVAGLCYALARNFKGNIAKGRDVSQPVAFVG
                     GVAANAGMRRAIKDVFSLTDDKFFIPEHFTAMGALGALYAIMDEPGLKKEFLGLGKLS
                     ERRARKTTEEAHEPLKISKENLQISYDLKHITEKTEVFLGVDVGSISTNLVVMDKEQN
                     LIAKRYLMTEGRPLEAVQRGLAEIGDEIGDMVEVVGAGTTGSGRYLTADFIGADIVRN
                     EITAQAEAAINLDREVDTIFEIGGQDSKYISVDNGVIVDFEMNKACAAGTGSFLEEQA
                     ERIGISIKEEFGDLALSSKRPVKMGERCTVFIESDIVHHQQKGANTDDIVSGLSYSIV
                     SNYLNRVVGDKRIGEKIFFQGGTAFNHGVVAAFEQTLKKPIKVPSHHDVTGALGVAIL
                     AMKEKTWEKSSFKGFDLSKRAYQVDTFECKGCENLCEIRKVTVENESPLFYGSRCEKY
                     DVVRRVEKSNIIDLFKLRDELLTDTYHQSCDGGEPIGVPKILNMWEFQPFWKAFLTEL
                     GFSPVFSDPTNKKTIRDGVENIIIETCFPIKLTHGHILNILNKGFKQVFIPSIINMRK
                     PSVDIPNTFACPYAQSIAYTSKASIDFEAFGARVLSPVVYFGGGSKITLQNLVSFGKQ
                     IKRSKKDIQRAFKAAMEAQELFYKKCSQIGQETLDALDENEKAMIIIGRPYNSADQGA
                     NLNIHRKLMDLGVKSLPLDMLPGIDRVETDESLQKMYWGYGQKILRAAKLIRDRKNLF
                     GIYVTNFGCGPDSFITHFFKRTIDGKPFLQLEIDEHSADAGIVTRLEAFLDSIKNSKI
                     RPVRQQSVVPEFSIGQRPKKIYIPYMADHAHVFASAFKACGQEAVVMDESDDTSVYWG
                     RKFTTGKECYPCILTTGDMVKVVKRKDFDPTNSAFFMPSGNGPCRFGQYNRFHRMVLD
                     ELGFEKVPIYAPNQDHRLYQECNILGGRFSRLGWRAIVATDLLYKMLHQIRPYEKTKG
                     MTEKVYRQALKDVSEALENGQNQVVKVLEESVNKMLAIEQLATKKPIVGIVGEIYVRS
                     NAFSNSNVIRTVEEFGGQVWLAPICEWISYVNFISNHSKNAHKPTIRDKMSMALTNYI
                     QHKDEEMLEHIFLQHLDYGKEPTVESIVEKASPYIHVSLEGEAILSVGKSVDFIEKGV
                     SGIINVMPFTCMPGTISSAIMRLLQKKYDVPIINIAYDGQGLTNITTRLEAFMHQVRE
                     HFSARHE"
     gene            4323..5630
                     /locus_tag="PK125_08465"
     CDS             4323..5630
                     /locus_tag="PK125_08465"
                     /inference="COORDINATES: protein motif:HMM:NF013741.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HPB38181.1"
                     /translation="MNNFIPEIILIVFLLILNGIFSAGEMAIVSSRKSKIKEMMKSGE
                     NKRANTLFNMKENPERFLSAVQIGITLFGTLASAIGGVLAVQYIEPLFKRIPLVGPFA
                     DTIALAIIVLVLTYIFLVLGELVPKYIGMNYKEKVALVIAPLFEFTSKLLFIFVNFLT
                     FSTMFVVKGLNLKKREEHIGEGEIKILLEEGRRKGIFDKTEEELIHGVFDFADRSVKE
                     IMVPKPNMYSINIDDGKDIVLEYIVTNEFSRYPVYKDYPDNIIGIVYQKDITRQMWQK
                     DEPFNLKNLLRKPFFVPDTMEVSRLLKEMQKRRTHMAIVVDEYGATVGIVTLEDIMEE
                     IFGEIMDETDEDDLIERRKDGAFIIDGSYSIRDLNNKLQLDLQESPDYETLGGFILTK
                     LQGIARGGEIVYHGPYRFTIGDVEGLRISKVKLERIKHSPKPR"
     gene            complement(5662..6621)
                     /locus_tag="PK125_08470"
     CDS             complement(5662..6621)
                     /locus_tag="PK125_08470"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF004344.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit alpha"
                     /protein_id="HPB38182.1"
                     /translation="MRYYLDFEKKLEPIERRIDEIERFYNVNDPRYAKEVSFLTKKIS
                     KLKKDIYSDLTNWQRSQISRHLNRPHTLDYITNLFSDFFEIHGDRKYKDDPAIVCGFA
                     RFDGIKLAVIGHQKGQDVREMAKRNLGMAHPEGYRKAMRIMELANRWGKPIVTFIDTP
                     GAFPGVGAEERGQAEAIASSICYMFSLSVPIVTIIIGEGGSGGALAIGVGDRVLMLEN
                     ATYSVISPEGCASILWRDGSKKSLAAESLKPTAYELFKLKVIDDVIKEPFGGAHRNWD
                     ETFLNVKGILEKHLMQLMEVPIEELQQKRYEKFRRMGIFEETK"
     gene            complement(6605..10057)
                     /locus_tag="PK125_08475"
     CDS             complement(6605..10057)
                     /locus_tag="PK125_08475"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF005298.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HPB38183.1"
                     /translation="MKEFVHLHLHTQFSLLDGAIRFDRLFEQANAFGMNACSITDHGN
                     MYGVIDFYFTARSFGIKPIIGCEAYLAPKSRFDQTRIRGEDNAYHIVLLAMNNDGYKN
                     LLKIISSAHLEGFYYVPRIDKDLLREHNAGLICLTACLQGEIPRQILKGDDNSVKKTI
                     DEYISIFDNRLFFELQDNGIPEQRIVNDTLIDLSRYYGIPIVATNDCHYLKKEEAKAH
                     ELLLCIQTGKTMLDKDRLSFTGDEFYFKSGDEIAQAFSNYPEALSNTLKIAEMCNVDI
                     DVGTYHFPEFNPGEGMEPNQYFETTCKKGFENRLQHIRPTYTEFTEELEQTYKKRLSY
                     ELDVIKNTGFASYFLIVADFIQYAKSSGIPVGPGRGSAAGSLVAYCLGITDIDPIKYD
                     LIFERFLNPERVNMPDIDVDFCKRGRDQVIEYVTEKYGTDNVAQIITFGTMQSKAAVR
                     DVGRALGMPYAEVDKIAKLITSGENGISKAINEEPQIKEMYQDDERVKELLDNAMVLE
                     GLARHASTHAAGIVISNKLLTEHLPLYKGKKGETVTQYHMKVIEKIGLIKIDFLGLET
                     LTIINDTISLLKDQGIELDLDKIPLDDKATYDLLSSGNTSGVFQLESRGMRELLVALK
                     PSKFEDIMPLIALYRPGPLKSGMVKEFTERKDNPSLVKYETPLLKDILEDTYGVIVYQ
                     EQIMKIATVLANFSIKDADALRKAMSKKIPEQLESYKEQFIKGATANGVTLSAAKNIY
                     EIILRFGEYGFNKSHSTAYGLISYQTAFLKTHYFVPFFAAILTSEVNDTDKMIQYITE
                     CRESGVEILPPDINKSQKSFTVVDNKIRYGLSGIKNVGDAAIDHLLSIREDLGEFSSF
                     VQFCGAVDTRKINKKVLESLAKAGCFDGMGLKRSQILNIIQDKMDKLQKKDHKNGYQQ
                     MDMFQNTISPVDTFEIPDLDELSHTEILNGEKEALGFYFSQHPLQPYENIIKQITNFN
                     SMALKDLDIAEDIGIVAIVSGCKEVITKRGDKMAYLTLEDTKGMLEAIVFPDPYSKNI
                     DIIKSDKPLFFYGSVEKNEEGPVKLKVKNICLLDDIIDEMRRIVKIKIQCDVFKKEDL
                     KRLRDVLVGLNGKASVLLELRMNGEKQVIELSEIRIDHTKVDVLFKHFSKGLEVEVLD
                     EILS"
     gene            complement(10300..11766)
                     /gene="guaB"
                     /locus_tag="PK125_08480"
     CDS             complement(10300..11766)
                     /gene="guaB"
                     /locus_tag="PK125_08480"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016291787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HPB38184.1"
                     /translation="MVDHNIKEGLTFDDVLLIPSFSDIMPQDADITTHLTRTIKLNIP
                     ILSAAMDTVTDAKAAICIAQEGGMGIIHRNMGVEEQAQEVEKVKKSESGMIVDPITIR
                     PEQKIRDAIELMARYRISGIPVTKGKKLVGIITNRDLRFETNLEETVENVMTKENLVT
                     VKENITLEESKAILHKHKIEKLLVVDGRFNLRGLITIKDIEKMRKYPNACKDQMGRLR
                     VGAAVGVAKDREMRIDALLRAGCDVIVIDTAHGHSKNVIDAIKDTRRNFRDIQLIAGN
                     IATPDACEALIKAGTDGVKIGVGPGSICTTRIVAGIGVPQISAIFDVEKVAKKYDIPI
                     IADGGIKFSGDITKALAAGANSVMIGNLFAGTDETPGEMVLYQGRTYKVYRGMGSLEV
                     MKEGKSRDRYCIAEDEIESKIVPEGIEGRVPYRGSLSIMVQQLVGGLRAGMGYVGCRT
                     IKELHEKRQFLRITSAGLKESHVHDVIITKEAPNYRIE"
     gene            11833..12915
                     /locus_tag="PK125_08485"
     CDS             11833..12915
                     /locus_tag="PK125_08485"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPB38185.1"
                     /translation="MVVWYKKLAIWNWFSIMQEVKIIKNIFPCEFVEMLGSENNPLLA
                     MVDVTVGFCTESSNTISVLENLNLVINKGEVFGLVGESGSGKTMTALSIMKLIPPPGR
                     ILSGKILFQGNDLLLLSEKQMESVRGQRIGMIFQEPMTSLNPVFRVGDQVAEILSVHT
                     EMKKKELAKKTIELLKDVGFDDPAQKYVQYPHQLSGGQRQRILIAMSIACNPALVIAD
                     EPTTALDVATEGQILCLLQDLVCKYQISMLFITHNLQIIKNIGSRIGIMYAGRIVEEN
                     RVDDFFREPLHPYSKGLLESIVGVRKASERLETIPGFVPKLTDLPAGCKFHPRCRNVM
                     AKCKKEEPPFINVGKSKWVRCYLYQN"
     gene            12885..13835
                     /locus_tag="PK125_08490"
     CDS             12885..13835
                     /locus_tag="PK125_08490"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HPB38186.1"
                     /translation="MGPMLSVSELEKTYEVRKTAFSNKKTTIRAVDRITFDLHKGKTL
                     GIVGESGSGKSTLARCLMLLEKPDSGVIKFFDQDLLKLKEESLKSLRKDIQIIFQDPY
                     SSLNPRKKIVNTLSEPLLFHQIANRQNVNEKAVEILHNVGLDEDSLYKYPHEMSGGQR
                     QRVAIGRALASEPSLLIADEPVSSLDVSIQAQIINLFVDIKNEFKISMIFISHDLNIV
                     RFISDDILVLYKGSIVEMGTRDEIFLHPIHPYTQMLIKASQGEFCAVQEKTDGAITDN
                     GCKYYRRCELADHFCGAKAPNLQGTEEHSVACFKATRHLI"
     gene            13966..14067
                     /locus_tag="PK125_08495"
                     /pseudo
     CDS             13966..14067
                     /locus_tag="PK125_08495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003112842.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
     gene            14092..14418
                     /locus_tag="PK125_08500"
     CDS             14092..14418
                     /locus_tag="PK125_08500"
                     /inference="COORDINATES: protein motif:HMM:NF012342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilin"
                     /protein_id="HPB38187.1"
                     /translation="MQEIIHSMGAVKTAVMAYYTESSTAPVAADADTIRTLLGVDVPI
                     KYLTSMVVTRGSDQGPSTITATLNNVIGGDTVNTTAILNSSADYKTWTWDQTNSTLPS
                     AYLPKN"
     gene            14608..>14953
                     /locus_tag="PK125_08505"
     CDS             14608..>14953
                     /locus_tag="PK125_08505"
                     /inference="COORDINATES: protein motif:HMM:NF018460.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HPB38188.1"
                     /translation="MVVLFHIFFFLLGCAIGSFLNVCIYRIPREKSIVKPPSACPGCE
                     KPIRFYDNIPILSYILLKGKCRDCGTRISMRYPLVELLTGIFFLLLYRKFDITFELLV
                     FMIFLSLLIAISV"
BASE COUNT         4290 a         3067 c         3159 g         4437 t
ORIGIN      
        1 accaaggagg gaacatgccc cgtcttcggg atgacacaaa atacttgacg ttaccccttc
       61 ctgttatggc ttgtattcaa ggaattagct tgataagcag ggcattttca ttataattaa
      121 caatagggtt taatcaaaaa aaaggacatc acttctttgt ggaggtaatg ttgtgaacag
      181 catcgggatt gatattggct ctgttagcat tagcctcgtt gttatgggtg aaacaggaga
      241 tatcctgaag agcctttatg ttcgccacaa aggcaggcct atagaagtta cagaatcagc
      301 cctcgaaaat ctattgaaag aatttgaaat cgattcagtt gcagtgacag ggacaggagc
      361 gaaagaaatc tctcttctta taggtgcatc tttcataaac gaaattgttg caatatccaa
      421 aggatttact catctgtatc ctcatgtgcg aagcataatt gacattggtg gagaggattc
      481 caagctcatc atattagaag aagaccgtac aaacaagaag cttcgtataa aggatttctc
      541 catgaatgct ctgtgtgctg ccgggacagg ttcttttctt gaccagcagg catcgagact
      601 atgtttcacc attgaggaat tcagtgaaat agctttaaaa tctgttaatc ccccgagaat
      661 agccggccgg tgcacagtat ttgcaaaatc agacatgatt cacctgcagc agatagcaac
      721 accagattat gagattgtgg ctggtctttg ttatgccctg gcaagaaatt ttaaaggaaa
      781 tattgccaaa ggtagggatg tttcacaacc ggtggctttt gttggcgggg ttgctgcgaa
      841 tgcaggtatg aggcgcgcaa tcaaagatgt tttttcgcta actgatgata aattcttcat
      901 tcctgagcat tttactgcca tgggcgccct gggtgcttta tatgctatca tggacgagcc
      961 aggattaaag aaggaatttc ttggcctggg gaaactgagt gaacgtcgag ccagaaagac
     1021 aacagaagaa gctcacgagc ctctaaagat ttccaaagaa aatctccaaa tatcatatga
     1081 cctgaaacat ataaccgaaa aaaccgaggt ttttcttggt gttgatgtgg gttcaattag
     1141 cacgaatctt gtagtcatgg ataaagagca aaacttaatt gcaaaaagat accttatgac
     1201 cgaaggcaga ccccttgaag ctgtccaacg aggtcttgct gaaatcggag acgaaattgg
     1261 agacatggtt gaggttgttg gagccggaac aaccggttca ggccgctacc ttactgctga
     1321 ttttataggt gctgatattg tgcgaaatga aatcacggcc caggctgaag cagcaattaa
     1381 ccttgacaga gaggtcgata ccatttttga aataggcggc caggattcca agtacatcag
     1441 tgtagataat ggggtcatag tggattttga gatgaacaaa gcatgtgctg cagggacagg
     1501 ctcattcctc gaagagcagg cggaaaggat tggaatctca attaaggagg agtttggcga
     1561 ccttgcactt agttcaaaaa ggcctgtgaa aatgggggaa cgctgtacag tttttattga
     1621 gtcggatatt gtccatcacc agcagaaggg tgcaaacact gatgatatag taagcggctt
     1681 gtcgtattca atcgtttcta attacctcaa cagggttgtt ggtgataaaa ggataggaga
     1741 aaagatattt tttcagggtg gaacggcatt taatcatggt gttgtggcgg cctttgaaca
     1801 aacactgaag aaacctatca aggtgccgtc tcatcatgac gtaaccggtg cattgggagt
     1861 tgcaattctt gcaatgaagg agaaaacatg ggagaagagc tccttcaaag gattcgatct
     1921 gagcaaacgt gcctaccagg tagatacatt tgagtgcaag ggctgtgaaa atctctgcga
     1981 aatcagaaaa gtgactgttg aaaatgaaag cccgttattt tatggaagca ggtgcgaaaa
     2041 atacgatgtc gtgagacggg tcgaaaagag taacatcata gatctcttta aattacgtga
     2101 tgaattactg actgatacct accatcaatc atgcgatggc ggtgaaccga tcggtgtgcc
     2161 caaaattctt aacatgtggg agtttcaacc attttggaag gctttcctta cagagcttgg
     2221 attttcgccg gttttctctg atccaacaaa caaaaaaacg attagagatg gtgttgaaaa
     2281 cataattatt gaaacgtgtt ttccaataaa actgactcac ggccacattt tgaacatcct
     2341 caacaaaggt tttaaacagg tttttattcc aagcatcatt aatatgagaa aaccatcagt
     2401 ggatataccc aatacatttg cttgtcccta tgcccagtca attgcttata catcaaaagc
     2461 atctattgat ttcgaggcat ttggtgccag ggttctttct ccagtagtat atttcggggg
     2521 aggaagtaag atcacattac aaaacctggt ctccttcgga aaacaaataa agaggtccaa
     2581 aaaggatatt cagagggcat ttaaggcagc catggaagcg caggagcttt tttacaaaaa
     2641 gtgttcgcag ataggacagg aaacattgga tgcccttgat gagaatgaga aggcgatgat
     2701 tataatagga cgaccttaca acagtgcaga ccagggggct aacctgaata tccacaggaa
     2761 actgatggat cttggtgtaa aatcactgcc cctcgatatg cttccaggta ttgacagggt
     2821 cgaaacagac gagagcctcc aaaagatgta ttggggttat ggtcaaaaga ttctacgtgc
     2881 agcgaaatta atcagagaca ggaaaaattt gtttggcatc tatgtcacaa attttggctg
     2941 tggaccggat tcgttcataa cgcatttctt caaaagaacc atcgacggaa aaccattcct
     3001 tcaactcgag attgatgagc acagcgctga cgcaggaata gtcacaaggt tagaggcttt
     3061 tctcgatagc ataaaaaact cgaagataag accagtacgt caacagagcg tggtgccaga
     3121 attcagcata ggacaacggc ccaaaaagat atatatccct tacatggcag atcatgccca
     3181 tgttttcgct agtgcgttta aagcatgtgg gcaagaagcc gtagtcatgg atgagtcaga
     3241 tgatacttcg gtttactggg gacggaaatt tacaacagga aaagagtgtt acccctgtat
     3301 cttgacaacg ggcgatatgg taaaagtagt taaaagaaag gattttgatc cgacgaatag
     3361 tgctttcttt atgccatcag ggaatggtcc ctgtcgattc ggccaatata acaggtttca
     3421 tcgaatggtc ctcgacgaac ttggttttga gaaggtacct atttatgctc caaatcagga
     3481 ccatcgccta tatcaggagt gcaacattct tgggggacgt ttttcgaggc tgggttggag
     3541 agctattgtt gccacagatc tgctttacaa aatgcttcat cagattcgcc cttatgagaa
     3601 aacaaagggt atgacagaaa aggtgtacag gcaggcactt aaagatgttt cagaggcatt
     3661 ggaaaatggt caaaatcagg ttgttaaggt cctcgaggaa tctgtcaata agatgttagc
     3721 catagaacag cttgctacta aaaaacctat tgttggtatc gtaggcgaaa tatatgtccg
     3781 atcaaatgca ttcagcaaca gcaatgtaat acgaacagtg gaagagtttg gcggacaggt
     3841 atggctggcc cctatctgtg aatggatatc atatgtaaat tttatcagta accacagtaa
     3901 aaacgctcac aaacccacaa tcagagataa aatgagcatg gcccttacaa attatatcca
     3961 gcataaagat gaagaaatgc tggaacatat ttttctacaa caccttgatt acggtaaaga
     4021 accaacggtt gaaagtatcg tggagaaggc aagcccctat atccatgtta gccttgaagg
     4081 agaagcgatt ctcagtgtgg gcaagagcgt tgattttatc gaaaaaggcg tttcaggcat
     4141 tataaatgtc atgccattta cctgtatgcc aggaacaatc tcgagcgcaa ttatgaggct
     4201 tttgcaaaaa aaatacgatg ttcccataat aaatattgca tatgatgggc aaggcctgac
     4261 caatatcact acgaggcttg aggcatttat gcatcaggtg agagagcatt tttcagcacg
     4321 ccatgaataa ttttatcccg gaaataatcc tgatcgtttt cctattgatt ttaaatggga
     4381 tattttcagc cggtgagatg gccattgtct cttcacgaaa gagcaaaatc aaggaaatga
     4441 tgaaatcagg ggagaacaaa agggcaaaca ccctctttaa catgaaggaa aaccctgaaa
     4501 gatttttgtc tgccgtgcaa ataggtatta ccttatttgg aactctggca tctgcaatcg
     4561 gcggtgtgct tgctgtccag tatattgaac cattgtttaa acgtattcca cttgtcggac
     4621 ccttcgcaga cacaatcgcc cttgcgatta ttgttctagt tttgacgtat atctttttag
     4681 ttcttggcga actggtaccc aagtatattg gaatgaacta taaagaaaaa gttgccctag
     4741 tgattgcccc cctttttgaa tttacgtcaa aattgttatt tatatttgta aacttcctta
     4801 cgttttcaac aatgtttgtt gtgaaggggc ttaacttgaa gaaacgtgag gagcatattg
     4861 gcgagggaga aataaaaatt ctccttgaag agggcagaag aaagggcatc ttcgataaaa
     4921 ctgaagaaga actgatacac ggtgtttttg attttgctga tcggtcagta aaagagataa
     4981 tggtaccgaa accaaatatg tattctatta atatcgatga tggcaaagat attgttttgg
     5041 agtacatcgt taccaatgag ttttccagat atcctgtata taaggattat ccggacaata
     5101 ttattggcat agtctaccaa aaagatatta cccgtcaaat gtggcagaaa gacgaacctt
     5161 ttaatttaaa aaatctttta agaaaacctt tttttgttcc tgacacaatg gaagtcagtc
     5221 gtcttctgaa ggaaatgcag aaaagacgca cccatatggc aattgtagta gacgaatatg
     5281 gagcaaccgt aggaatagtt acactcgaag acataatgga agagattttt ggtgaaatta
     5341 tggatgaaac agatgaagac gacttgattg aacgtcgaaa ggatggtgca ttcatcatcg
     5401 atggctctta ttctatacga gatctgaaca ataaacttca gcttgacctc caggagtccc
     5461 ctgattatga aacgctggga ggtttcattc taacaaaact gcagggtata gctcgaggtg
     5521 gagagattgt atatcatggt ccctatcgtt ttactattgg ggatgttgag ggtttgagaa
     5581 tcagtaaagt aaagttggaa agaataaagc acagcccaaa accacgataa tggttcacgt
     5641 tactgtgcgc cactatctgg cttattttgt ttcctcaaaa attcccattc ttctaaactt
     5701 ttcatatctt ttctgctgaa gttcttctat gggaacctcc ataagttgca tcaggtgttt
     5761 ttccaatata cctttcacat tcaaaaacgt ttcatcccag tttctgtgag ccccgccaaa
     5821 aggttccttt attacatcat cgataacttt gagtttaaaa agctcatatg cggtgggttt
     5881 aagggattca gcagccagag atttctttga gccatctctc cacaatatag acgcacatcc
     5941 ttcaggagag atcacggaat acgttgcgtt ttcaagcatt aacacacgat ctcccacacc
     6001 aatggcaagt gctcctccac tgccgccttc accaataata atcgtgacaa ttggcactga
     6061 cagagaaaac atatagcaaa tactgcttgc tattgcttcg gcctgtccac gctcttcagc
     6121 gccaactcct gggaaagcac caggggtatc aataaaggtt acgattggct tcccccatct
     6181 gttggccagt tccattatgc gcatagcctt tctgtaccct tcaggatgag ccataccaag
     6241 attcctcttt gccatttctc tgacatcttg tcctttctga tgtcctataa cagccagttt
     6301 aattccatca aaccttgcga agccacaaac tatggctgga tcgtctttat attttcgatc
     6361 gccgtgaatt tcaaaaaaat ccgaaaaaag gttcgtaatg tagtccagcg tatggggcct
     6421 gttcaagtgc ctcgagattt gggatctttg ccaatttgtt agatcgctat aaatatcttt
     6481 cttgagcttt gatatttttt ttgtcaggaa ggaaacttct tttgcataac ggggatcatt
     6541 cacattgtag aacctttcta tctcgtctat ccgacgttcg atcggttcga gtttcttctc
     6601 aaaatcaaga taatatctca tctaatacct caacctctaa gccttttgaa aaatgtttaa
     6661 ataacacatc gaccttggtg tggtcaattc ttatttcact gagttcaata acctgctttt
     6721 caccattcat cctcaattct aaaagaacgc tggccttgcc attgagcccc acaagcacat
     6781 ctctgagtct tttgagatct tcctttttga acacatcaca ttgtatcttt atcttcacta
     6841 ttcttctcat ttcatcgatt atgtcgtcaa gaagacagat attttttacc ttgagtttta
     6901 ccggaccctc ttcgtttttt tctactgatc cgtaaaaaaa gagcggttta tcgcttttaa
     6961 tgatatcaat attttttgaa tacggatcag gaaagacaat ggcctctaac atccccttcg
     7021 tgtcttcaag ggttaagtag gccattttgt cgcctctttt cgtaatgacc tctttacacc
     7081 cgctcactat tgccacaata ccaatatctt ctgcaatatc caaatctttt aatgccatgc
     7141 tgttaaagtt tgtaatttgt ttgattatat tctcataggg ttgtaatgga tgctggctga
     7201 aataaaaacc cagtgcttcc ttctccccgt taagtatttc ggtatgagaa agttcatcca
     7261 ggtccggaat ctcaaatgta tcaactggtg atatggtatt ttgaaacata tccatctgct
     7321 gatagccgtt tttgtgatcc tttttctgga gcttatccat tttatcctgg atgatattga
     7381 gtatttggga gcgcttaagc cccattccat caaaacagcc tgcttttgca agactttcga
     7441 ggactttttt gttaattttc cgtgtgtcga cagcaccgca gaattgaaca aaggaagaga
     7501 actcaccgag gtcctctctt atgctcaata aatgatctat cgcagcatct ccaacatttt
     7561 ttattccaga caagccatat ctgattttgt tgtccacaac agtaaaggat ttttggctct
     7621 tgttgatatc cggcggtaga atttcaactc cgctttcacg acactctgtt atatactgga
     7681 tcatcttatc agtgtcgttc acttcgcttg taagtatggc tgcaaaaaac ggcacaaagt
     7741 agtgtgtttt aaggaatgca gtctggtaag agattagccc gtacgctgtg ctgtgagact
     7801 tattgaatcc atattcccca aaacgaagta tgatttcgta gatatttttt gcagcactga
     7861 gagtgacacc gtttgctgtt gcacctttaa taaattgttc tttatagctt tcgagttgtt
     7921 cagggatctt cttactcata gcttttctta acgcatcagc gtcttttata gaaaagtttg
     7981 cgagaactgt ggcgattttc attatctgtt cctgatacac gatcacgcca taggtgtctt
     8041 caagaatgtc tttaagcaga ggtgtctcat acttaacgag ggaagggttg tctttcctct
     8101 ctgtaaattc tttgaccatg ccgcttttta gtggaccagg cctgtaaagg gcaatcaggg
     8161 gcatgatgtc ttcaaatttt gacggcttga gagccacaag aagctctctc atgcctctac
     8221 tttcaagctg gaacacgcct gacgtgtttc ccgacgaaag aagatcataa gttgctttat
     8281 catcaagggg aatcttgtcc agatcaagtt caatgccctg atcctttaga agagaaatgg
     8341 tgtcattaat gatagtgagc gtttcaagac ccagaaaatc aatcttgatc agcccaatct
     8401 tctcaataac cttcatatgg tattgagtaa ctgtttcacc ctttttccct ttatataacg
     8461 gaagatgttc tgtgagaagt ttatttgaga ttacaattcc tgcagcatgg gtcgaagcat
     8521 gccgtgcaag cccctccagg accattgcgt tgtcgagaag ttccttgact cgttcgtcat
     8581 cctggtacat ttccttgatt tgaggctctt cattgattgc ctttgagatg ccgttctccc
     8641 cggaagtaat cagtttggct atcttgtcta cttctgcata gggcattccc agggcgcggc
     8701 ctacatccct gactgcagcc ttggactgca tggtcccaaa tgttatgatt tgtgcaacat
     8761 tgtctgttcc gtatttttct gtaacatatt cgatgacctg gtctcttcct cttttacaga
     8821 aatccacatc aatatccggc atgtttaccc tttcgggatt cagaaaacgc tcaaaaatca
     8881 gatcatattt tatgggatca atatctgtga ttcccaaaca gtaagccacg agactgcccg
     8941 cagctgaacc tctaccgggg ccgaccggta tgccggatga ttttgcatac tggataaagt
     9001 ctgccacaat gaggaagtaa ctcgcaaaac ctgtattttt gattacatcc agttcgtagg
     9061 agagtctttt cttgtatgtt tgttcaagtt cttctgtaaa ttcggtgtag gtaggacgga
     9121 tgtgttgaag tctgttttca aaaccttttt tacacgttgt ctcgaaatac tgattcggtt
     9181 ccatgccttc gcccgggtta aactcgggaa agtggtaggt gcccacatcg atgtctacat
     9241 tgcacatctc tgcaatcttc agtgtatttg acagggcctc ggggtaattt gaaaaagctt
     9301 gcgcgatttc atcccctgat ttgaagtaga attcatcgcc cgtgaaactg agacgatcct
     9361 tgtccagcat tgtcttgcct gtctgaatgc acagtaataa ctcgtgagcc tttgcctctt
     9421 cttttttcag ataatggcag tcattggtag cgacaatggg aataccataa tacctggata
     9481 gatctatgag tgtgtcattg acaatccttt gttcaggtat gccattatcc tgaagctcaa
     9541 agaaaagcct gttgtcaaaa attgagatgt actcatcgat agttttctta actgaattat
     9601 catcaccctt caggatttgc cttggtatct caccttgaag gcatgctgtt aaacatatga
     9661 ggcctgcatt atgttcccgt aacaaatcct tgtcgattcg cggaacatag tagaatcctt
     9721 ccaggtgcgc agagctgatg atttttaaaa ggttcttata accatcattg ttcattgcaa
     9781 ggagaactat gtgatacgcg ttatcctcac ccctaatcct tgtctggtca aaccttgatt
     9841 taggcgcaag gtatgcttca cagcctatta ttggttttat accaaaacta cgggcggtga
     9901 agtaaaagtc tatcacccca tacatattcc catgatcggt gatactgcaa gcgttcatgc
     9961 cgaaagcatt tgcctgttca aaaagacgat cgaacctgat cgctccgtca agcaggctaa
    10021 actgtgtatg gagatgtaag tgaacgaatt ccttcattta ttccctgtgg gcaggcatcc
    10081 cgcctgtctg tcaaaatgtc ttctgaagcc caagcagatt ccaagaacag gaattcctaa
    10141 agcagagttg aacagttccc caactagagc atcagattca tgagcagtat ctggtatcct
    10201 tgaaagaatg ctatttgctg gttttaatga gattatttcc tgtaagacga atttgtgagg
    10261 aatgacatcg cagttcacag caagctctcg cacactggtc tactcaattc tgtagttagg
    10321 tgcttctttt gtaataatta catcgtggac atggctttct tttagacctg cagaagttat
    10381 cctgagaaac tgcctttttt catgcaactc tttaattgtt ctacatccaa cgtatcccat
    10441 cccggcccgg agccccccca caagctgttg caccattatc gataaagagc ctctgtaagg
    10501 aacacggccc tcaatgccct caggaacgat tttagactca atttcgtctt cagctatgca
    10561 gtaacgatca cgcgacttcc cttccttcat aacctccagg gaccccattc cccggtagac
    10621 tttgtaggtt cttccctgat agagcaccat ttcgccaggg gtttcatctg ttccagcaaa
    10681 taaattgcca atcatcactg aattggcacc cgccgcgaga gctttggtga tgtcaccgga
    10741 aaatttaatg cctccatcag caatgattgg gatatcgtac ttttttgcca ctttttcaac
    10801 atcaaaaatg gcagatatct gtggaacccc gatgcctgca actattcttg ttgtgcatat
    10861 cgatcccggt cctacaccga ttttcacacc gtcagttcca gcttttatta aggcctcgca
    10921 cgcatccggt gtggctatgt ttcctgcaat aagctgtata tccctaaaat ttcttcttgt
    10981 atcttttatg gcatcaataa catttttaga atggccatga gctgtatcga ttacaataac
    11041 atcacacccc gccctgagga gtgcatcaat cctcatctcc ctgtctttag ccacacccac
    11101 tgcagctccc acgcgaagcc tgcccatctg gtctttacac gcattgggat actttcgcat
    11161 cttctctata tctttaatgg taataaggcc tctcagatta aaacgcccat cgactaccaa
    11221 aagcttctca attttatgct tatgaagaat ggctttcgat tcttctaagg ttatattctc
    11281 cttgactgtt acgagatttt cctttgtcat aacgttttca acggtttctt caaggtttgt
    11341 ctcaaatcgc aaatcccggt ttgttattat tccaacaagt ttttttcctt ttgtaactgg
    11401 aatccctgat attctgtacc gtgccataag ttctatggca tctcttattt tctgttcagg
    11461 gcgaatcgtt atgggatcaa caatcatacc actttcagac ttcttaactt tttcaacctc
    11521 ctgagcctgc tcttccacac ccatattcct gtgaataatg cccatcccac cttcttgggc
    11581 aatgcagatt gctgcctttg catcggttac agtatccata gcagcactga ggattggaat
    11641 gttcagttta attgtccttg ttaaatgggt ggtaatatca gcatcttggg gcattatgtc
    11701 gctaaaagat ggtatgagga gcacatcatc gaaggtaagg ccttctttta tgttatggtc
    11761 gaccatggct tactccttta caaagatcat gaaaatagtc agtttatttg ccgttcactg
    11821 aaggaagttt taatggttgt atggtataaa aagcttgcca tttggaattg gtttagtata
    11881 atgcaagagg ttaaaataat caaaaatatt ttcccttgcg aatttgttga gatgttaggt
    11941 tcagaaaaca atccattgct tgctatggtt gatgtaaccg ttggtttttg tacggaatca
    12001 tccaacacta tcagtgtatt agaaaattta aaccttgtca taaataaagg agaggtgttc
    12061 gggttagttg gcgagagtgg ttctgggaag acgatgacag cgctctcaat aatgaaactg
    12121 attccccctc cgggaagaat tctgagtgga aaaatactgt ttcaaggcaa tgatttgctg
    12181 ctgttgtctg aaaaacagat ggaatcagtc agaggtcaaa gaattggtat gatttttcaa
    12241 gaaccaatga catcactgaa cccggttttc agggtagggg atcaggttgc ggaaatcctc
    12301 tctgtccata ctgaaatgaa aaaaaaggaa ctggcaaaaa aaaccattga gctcttgaaa
    12361 gatgtgggtt ttgatgatcc ggcacagaaa tatgtccagt atccccatca attgagcggc
    12421 ggtcaaagac agagaattct aattgccatg tccattgcct gcaacccggc cctcgttata
    12481 gccgatgaac caaccacggc ccttgacgtt gccacagagg gacagattct gtgtttactc
    12541 caggaccttg tctgtaagta ccaaatatcg atgctgttca ttacccacaa ccttcaaatt
    12601 ataaaaaata taggttcaag aattggaatc atgtatgccg gcagaattgt agaagagaat
    12661 agagtcgatg atttctttcg tgagcctttg cacccatata gtaagggact actcgaatca
    12721 attgttgggg taagaaaggc ttctgaaaga ttggaaacga tccccggctt tgtcccaaaa
    12781 ctgacggatt tacctgctgg ttgtaaattc catccccggt gcaggaatgt tatggcaaaa
    12841 tgtaaaaaag aagagccacc ttttattaat gttgggaaaa gcaaatgggt ccgatgctat
    12901 ctgtatcaga attagaaaaa acgtacgaag taagaaaaac agctttttct aacaaaaaaa
    12961 cgaccataag agcggtcgac aggataacct ttgatctgca taaaggaaaa acgcttggca
    13021 ttgttgggga aagtgggtcc ggaaagagca ctctcgccag gtgcctcatg cttcttgaaa
    13081 aaccggatag cggggtcatt aaatttttcg atcaggacct tttaaaatta aaggaagaaa
    13141 gcctgaaaag cttacgcaag gacattcaga tcatttttca ggacccttat tcttccctca
    13201 atccaaggaa aaagatcgta aatacgctaa gtgaaccttt attattccat caaattgcaa
    13261 acagacaaaa cgtcaatgaa aaggctgttg agatcctcca caatgtcggg ctggatgaag
    13321 attctcttta taaatacccc catgaaatga gtggtggcca aaggcagagg gtggcaattg
    13381 ggagggcgct tgcatctgag ccatcccttt taattgctga cgaacctgtc tcatctcttg
    13441 atgtctctat tcaggcccaa attataaatc tttttgttga catcaagaat gaattcaaga
    13501 tctcaatgat ttttatttcc catgacctca acattgtccg gttcatatcc gatgatatcc
    13561 tggttttgta caagggaagc attgttgaaa tggggaccag ggatgagatt tttctccatc
    13621 ccattcaccc atacacgcaa atgctcatta aggcatcgca gggtgagttt tgtgctgttc
    13681 aggaaaaaac agatggagct atcactgata atggatgcaa gtattaccgg cgatgcgaat
    13741 tggctgatca tttttgtgga gcaaaggctc caaatttaca aggtacagag gaacactctg
    13801 ttgcgtgctt caaggcaacc aggcatttaa tttgatcatg gttaacctta tctagttctg
    13861 ttcttgcgat ggtattatta gtgtgacaat tgtcattgat cctctaaagt tatcctatta
    13921 agatacgata ctgttatata aatatatcct aaacggaggt gttacatgtt aaaggttcaa
    13981 agaaattcta aaggttttac gttgattgag ttactcatcg ttattgcgat catcggtatt
    14041 ctggctgcga tcgcaatccc tgcgtacact ggctacacaa gaaaagccag ggtgcaggaa
    14101 ataatccact ccatgggtgc agttaagact gctgtaatgg cttattacac ggaatcatca
    14161 actgcaccgg tagctgctga cgcggatact ataagaacgc ttctcggggt tgatgtccct
    14221 atcaagtatt taacttccat ggttgttaca cgcggttctg atcaaggtcc ttcgaccatc
    14281 acagcaacat taaacaatgt tattggcggt gacacagtga acacaactgc tattcttaat
    14341 tcttctgccg attacaagac atggacatgg gatcaaacaa atagtacgtt gccatcagca
    14401 tatctgccca agaattaaag gatgatttgg gcaaaaaggg ggcatcgatt gccccctttt
    14461 ttaagctttt gtttaatgta atttggtcag tgagggattt cagatggtac aaagcacttt
    14521 ttggagatca aaattggtgg gaatgcttat ccattataat aggttagtac ttttcgatga
    14581 tagcaaaaaa agaagagacg gatagagatg gttgtacttt ttcacatatt ttttttcctc
    14641 ctgggatgcg caattggcag ctttctcaat gtctgcatct atagaatccc cagagagaaa
    14701 tcgatagtaa aaccaccctc tgcctgccct ggttgtgaaa aaccgataag attttacgat
    14761 aatattccca ttctcagcta tattctgcta aaaggtaaat gcagagattg cggaactaga
    14821 atttcgatgc gatatcctct tgttgaacta ttaacaggca ttttttttct tttgctctat
    14881 cgaaaatttg acattacctt tgaactactt gtgttcatga ttttcttatc cctgctgatt
    14941 gcaatctcgg tca
//
LOCUS       DAOOMC010000073        14789 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdus sp. isolate AMR_MDS_2685
            NODE_4320_length_14789_cov_6.778064, whole genome shotgun sequence.
ACCESSION   DAOOMC010000073 DAOOMC010000000
VERSION     DAOOMC010000073.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864760
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdus sp. (wastewater metagenome)
  ORGANISM  Syntrophorhabdus sp.
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae; Syntrophorhabdus.
REFERENCE   1  (bases 1 to 14789)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14789)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,729
            Genes (coding)                    :: 2,708
            CDSs (with protein)               :: 2,708
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 7 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14789
                     /organism="Syntrophorhabdus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4320_length_14789_cov_6.778064"
                     /isolate="AMR_MDS_2685"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2699756"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            252..386
                     /gene="rpmH"
                     /locus_tag="PK125_08510"
     CDS             252..386
                     /gene="rpmH"
                     /locus_tag="PK125_08510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000831329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HPB38189.1"
                     /translation="MKRTFQPHNKSRKRTHGFLVRMRTASGRNIIRRRRAKGRKQLTV
                     "
     gene            383..769
                     /locus_tag="PK125_08515"
     CDS             383..769
                     /locus_tag="PK125_08515"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: protein motif:HMM:NF013022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030677 - ribonuclease P complex
                     [Evidence IEA];
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_function: GO:0042781 - 3'-tRNA processing
                     endoribonuclease activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HPB38190.1"
                     /translation="MKAAKPVDINYNTDFNPMANTFFKDERLAKGDFRGIKWKKFAET
                     PHFLLLVYGNDKGQKKFGVTIRKKTGGAVVRNRMRRLVKEFYRLNKDLFPDRHNNLLK
                     IKRVPERLTWTETDKELHALLQVHTL"
     gene            715..975
                     /gene="yidD"
                     /locus_tag="PK125_08520"
     CDS             715..975
                     /gene="yidD"
                     /locus_tag="PK125_08520"
                     /inference="COORDINATES: protein motif:HMM:NF013932.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HPB38191.1"
                     /translation="MDGDRQRTARVIAGTYPLKKIAICLLDIYKTCISPFIPTQCRFY
                     PTCSSYMQEAIEKKGIFRGLACGIMRILRCHPFSRGGYDPVK"
     gene            988..2580
                     /gene="yidC"
                     /locus_tag="PK125_08525"
     CDS             988..2580
                     /gene="yidC"
                     /locus_tag="PK125_08525"
                     /inference="COORDINATES: protein motif:HMM:TIGR03593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0051205 - protein insertion into membrane
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HPB38192.1"
                     /translation="MEKRTMIALLLTIVLLFFFQMYFAPRQPVQEAPQAKPEEKAQTK
                     TDAPSQPKVTGSVPLTKTEAPKPEETKATQRFLVETPLFKVTFTDLGGGIESVKLNQY
                     KEAVKGVGGKELVQDIKPYSYTPKVSKTTNGSTVNDRTIFKPDKKRMSIQDKPETIIF
                     SGTLTDGKRIRKIYTFYPDKYTIDMKIEVEATDADKAMVDFTVISGTNPNSYVFKGPF
                     IYNGKKFEQIDKIDKDIAVDKTYTYAGLDEGFFAFIWLPSEDSHFPLTIHKAENNVPV
                     LRMSMDKGMATGRLFFGPKQTDVLKSLDVQAQKIIDFGWFDVIAKPLIWALNMMNRVT
                     RNYGINIIILTIFIKIIFYPLTVKSSTSMKKMQKLQPMIVKLKEKYKDDKTKLNQEMM
                     AIYKREGVNPMGGCLPMIIQIPVFFALYKALSGAIELRHAHFLWWMNDLSAPEDLFTL
                     SVAGYALPIRILPLIMGITQVLQQKMTPTTADPMQEKIMLLMPIVFTFLFWGFPSGLV
                     LYWLVNNVISIAQQYYINKKAT"
     gene            2594..3340
                     /gene="jag"
                     /locus_tag="PK125_08530"
     CDS             2594..3340
                     /gene="jag"
                     /locus_tag="PK125_08530"
                     /inference="COORDINATES: protein motif:HMM:NF041568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_process: GO:0051510 - regulation of unidimensional cell
                     growth [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding cell elongation regulator Jag/EloR"
                     /protein_id="HPB38193.1"
                     /translation="MNSIEIEGKTYEEAIKKASEALNVEEKDLDVDVKEVDTKGILGL
                     LGSKKIRITARIKGTASPVAEDKPVSENTIVPEDAPVSEDTPEQYGTKFLTDVATITG
                     LHFNIKVSKISEKLDRLLFMIQCDNGDVFVGKDGEILEALQYLLRLAIAKHYKQSAKI
                     LVDINGFREKRKKALTIMAKRLADRVRRTGRRQTTDPLNPYERRIIHTLFKHNKNIAT
                     KSEGDGHIKKVIISPSSNRPARAGYRHGNR"
     gene            3327..4694
                     /gene="mnmE"
                     /locus_tag="PK125_08535"
     CDS             3327..4694
                     /gene="mnmE"
                     /locus_tag="PK125_08535"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HPB38194.1"
                     /translation="METVDTICAISTPPGEGGIGIIRISGPKAHELLFKIFRPAKTKK
                     NYLPRTLYLGKITNSDSNEKIDEVYGVLLKAPKTYTREDMAEIHSHGGFASQQAILSL
                     LIRCGARLAEPGEFTKRAFLNGRIDLLQAEAVLDIIQSETNEELRCAVEHLEGKLSSK
                     INTIKNNVKGVLVEIEALIDFPDEEIDVDMEQCLTILRRTHKNVEKLIKSYYEGRAIQ
                     HGLEALIIGKANVGKSSLLNALLLKEKAIVTPLPGTTRDLIEDILHIKGIKVRIVDTA
                     GLRKPKDIVEQEGIDRVKNKVPMTDLIIWVLDGSDAYTSEDEDIYTAIAGTNILVAIN
                     KIDLPQKLDNTKILSRGLEFYNISALRGSGLEELKNGIYRKLMGKKGKRAGLLITNVR
                     HREVLQKVYEAIGRAINCQENQEPVEFAAFELREALFNLGEITGETCPEDILHDIFSR
                     FCIGK"
     gene            4980..6395
                     /gene="atpD"
                     /locus_tag="PK125_08540"
     CDS             4980..6395
                     /gene="atpD"
                     /locus_tag="PK125_08540"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020045332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HPB38195.1"
                     /translation="MNEHERTIKSGINDPQVGTVTSVRGSVVDASFPGKLPPLHNLLR
                     TGDDGRIVIEVITHLNSEMVRGIALVPTQGLAYGSSIIDTGNALRVPVGKRLLGRVFN
                     VFGETIDKMDPVSGGEWRSIHRDSIPLSQQSTVSEIFETGIKAVDVLAPLERGGKAGL
                     FGGAGVGKTVLITEMIHNMFGRHEGVSLFCGIGERCREGEELYRELRDAGVLDHTVMV
                     FGQMNEPPGARFRVGHAAMTMAEYFRDDMQQDVLLLIDNIFRFIQAGMEVSGLMGYLP
                     SRLGYQPTLGTEIAKLEERICNTATGAITSVQAVYVPADDFTDPSAVHTFSHLSASIV
                     LSRKRASEGLLPAIDPLQSGSRMLVPHLVGKKHYHVAQEIRKTLATYEDLKDIIAMLG
                     IEELSKEDRKTVYRARRLERFLTQPFFVTEQFTGLKGKTVSREDAIEGCERILNDEFS
                     EYPERAVYMIGTIHEAKIHES"
     gene            6385..6780
                     /locus_tag="PK125_08545"
     CDS             6385..6780
                     /locus_tag="PK125_08545"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF004871.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HPB38196.1"
                     /translation="MNLKILLPAGILLNEQVNKVIAEAVNGSFCLLPRHIDFLAALAP
                     GILSFVSAEGKEEFLAVDEGVLVKAGPEVLVSSTRAVRGGELGQLKDTVERVFRHIDD
                     QEKSARSTMAKLEADLVRGFMELGERGYK"
     gene            6767..7087
                     /locus_tag="PK125_08550"
     CDS             6767..7087
                     /locus_tag="PK125_08550"
                     /inference="COORDINATES: protein motif:HMM:TIGR02230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AtpZ/AtpI family protein"
                     /protein_id="HPB38197.1"
                     /translation="MATSSILRDTERMSEDYLSKKIGKKEARKLKARSERRYSALVKG
                     LRMTGLVGWTVATPTLIGVAIGKWLDRAWPANFSWTLTLLMAGLILGCFHAWYWLKRE
                     SKND"
     gene            7080..7382
                     /locus_tag="PK125_08555"
     CDS             7080..7382
                     /locus_tag="PK125_08555"
                     /inference="COORDINATES: protein motif:HMM:TIGR03165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase subunit I"
                     /protein_id="HPB38198.1"
                     /translation="MTESIAFNVSIAFAAGLLLGLFYFGSLWMTVQGLVKVKKPALLS
                     FVSFFVRTGLTLTGFFFVMSGHWERALACMFGFLVMRKILTHRYGLDHQTLQRRKG"
     gene            7391..8137
                     /locus_tag="PK125_08560"
     CDS             7391..8137
                     /locus_tag="PK125_08560"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF004481.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HPB38199.1"
                     /translation="MEILHLDQISPDAVVLWKWKFFTLNATILYTWIIMAILTVGSWL
                     ATRNLSTEPETSRWQSLLEVVVVGMRNQIGEICQQDAGRYLPFIGTLFLFIAISNLLG
                     FVPGYAPPTSSLSTTAALAVCVFVAVPLYGMWDRGPIGYLKHFAEPSLFIMPFHILGE
                     VSRTVALAVRLFGNIMSGSKVVGILLAITPLLFPIVMQVLELLIGLIQAYIFAILSTV
                     YIASALKAHEEKESFAHSSGSLSGSTTVSG"
     gene            8192..8461
                     /locus_tag="PK125_08565"
     CDS             8192..8461
                     /locus_tag="PK125_08565"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF009998.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HPB38200.1"
                     /translation="MDVKTLVGMISIIIAGMTMFVGSISPALGMARAVAQALSSIAQQ
                     PDEANTITRTLFIGLAMIESLAIYCFVISMILIFANPFWRYVTSQ"
     gene            8472..9248
                     /locus_tag="PK125_08570"
     CDS             8472..9248
                     /locus_tag="PK125_08570"
                     /inference="COORDINATES: protein motif:HMM:TIGR03321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB38201.1"
                     /translation="MLIDWFTVVAQIINFLILVFLLKHFLYGRIINAMDQREARITSR
                     LEEAETQRKEAENEAAMFKLKNQYIESQREEMISSAKEESESFKKDLMQKAREEIDSI
                     QARWHETVEKQKIAFLKDLGQRATKEVFAVTRQVLKDLADKDLEQQIVDVFIRRVEKL
                     SQEERRLISESVNGSGIILITSAFQIPDEGQVKILNTIKEYINNECQIDYQIASDVIS
                     GIELRTVGHKIAWSFDNYLASLETNVLEAMELGSRSEGAE"
     gene            9245..10774
                     /locus_tag="PK125_08575"
     CDS             9245..10774
                     /locus_tag="PK125_08575"
                     /inference="COORDINATES: protein motif:HMM:TIGR03324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alternate F1F0 ATPase, F1 subunit alpha"
                     /protein_id="HPB38202.1"
                     /translation="MSADELHTVLDSTFSYIEHELESFKPDVKADEVGTVKSVGQGVA
                     QVEGLPRAKSEELLRFTGNLLGTVFNVDPSGMGVVLLDQSADLKAGSEVYPTGRVLDV
                     PVTEALLGRVIDAMGRPLDNQGPIQSTERLPIEREAPAIMDRAPVVVPLQTGLKVVDA
                     LIPIGRGQRELILGDRQTGKTAIVVDTIINQKNEDVICIYCAIGKQSAAVARFIADLK
                     KYGAMEYSMVVLATGEDTPGLQFVAPYAATTMGEYFMRKGHDVLVIYDDLTAHARAYR
                     ELSLLLRRPPGREAFPGDIFYIHSRLLERSTHLKEELGGGSLTALPIVETEAQNMSAY
                     IPTNLISITDGQIYLSPDLFQKGILPAVDVGKSVSRVGGKTQLAAYRAVAGDLRLSYS
                     QFEELEAFARFGTRLDEATLKTLERGHRVREVLKQSQYEPLSVAEQIACFLAVTNGVF
                     DNIEIDAVDEAQTTIRKNLVQQLPDVCHRIEKGEELTAEDKESILKMAKDTVTFHYGI
                     V"
     gene            10776..11660
                     /locus_tag="PK125_08580"
     CDS             10776..11660
                     /locus_tag="PK125_08580"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF012456.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HPB38203.1"
                     /translation="MPSIETLKRRIRTAGDLRSLVRTMKALAAVSIRQYERAVESLAD
                     YNRTVEMGFQILMREKETSLLRERGTKKCLGAVVFGSDLGMCGQFNEQIAAYALEEMK
                     KLGCEPSDRSCVVVGARVTGYLEDGNQKVDKELSVPGSMSGITLTVQEINEILSDWWM
                     SKKINQLYLFYNNHISGSQYRPHSVRLLPLDLEWLQDIKMRKWPTKVLPMYTLEWQKL
                     FPLIVRQYVFVSLFQATAESLASENASRLAAMQGAEKNIDDVLSGLKLQFHQERQMAI
                     TDELLDIVAGFEALAEPE"
     gene            11776..13527
                     /locus_tag="PK125_08585"
     CDS             11776..13527
                     /locus_tag="PK125_08585"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HPB38204.1"
                     /translation="MKKTTKDNNQIQAKNSEHIQEILSQNLNIYRLIAENMYDAIYTL
                     DTNVNFTFVNDVALKRSGYTRKWFIGRSFLNFVIPENKNMVQKSFKSVMMGKVVPAYE
                     LSYINSKGKSLWIEINATPLVDNGNIIGVLVVSRDITERKRNEEELKLYRNNLESLIE
                     GRTAELRIANEQLQIEIAQHKKTEEALRSSEAYYRTIFHNTGTAIIIMEDDTTISLSN
                     AECEKFVGYRASDLEGKRRAVEFVARQDLERILSYNELRRQDPEKAPRSYELKIVDRH
                     GNVRDVLITIASIPHTNKTIASFLDITEQKKMEAALKASEEKYRNIFENAVEGIFQIG
                     INGEILSANPSFSHLFGYKSPQEIMRAVKDIRYEIYADESQRNELRRLLDKQGFVRNF
                     EVICRRKDGQRIWISVNIRAVKDNNSKFLFYEGTLVDITERKMIQDDLEKKSRSLEET
                     NAALRVLLKQREEDKTDLEEKVLHNIKELVLPYIDKLRAGQQNRDTVIIDIVESNLNE
                     ILSPFIKSMASKYANFTPKEIQIADLMKKGKSTKEISTILNLSPRTIDIHRYNIRRKL
                     NINKKKVNLQSYLLTLT"
     gene            13745..14578
                     /locus_tag="PK125_08590"
     CDS             13745..14578
                     /locus_tag="PK125_08590"
                     /inference="COORDINATES: protein motif:HMM:NF028121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter TatT component family protein"
                     /protein_id="HPB38205.1"
                     /translation="MNHLGDALAKSGSTFSSDNDPDMIKAAAPFSLKLMESVLAETPE
                     HTGLLLALSSGFTQYAFAFVVQEADETEENNLIRAMETRARALRLYLRARDYGLRALD
                     IRHPGFAEALHGEPEKVVQDLKKGDVPYAYWTAVSWAGAISMAKNNADLIADLPKVEA
                     LIDKALALDESFDNGAIHSFLIAYDMNRASGTGNPEENARKHFSRAVELSDGKQAGPY
                     VTMAESVSLPKQDKKEFILFLERALAINADAKPEWRLANVIMQKKARWLLQRTDKLFV
                     N"
BASE COUNT         4404 a         3249 c         3416 g         3720 t
ORIGIN      
        1 tgactctgtc ttcctcgggt ggaagggaag agattgcaag aatgagccga ccaacagatg
       61 caccttcata atggagaaaa acgtaaaagt aaaagccatc ttcgaaggtg cttcacaata
      121 acatgaaata agggtaaata tctcttttct atcgcctatg acccgtgtcc attggccatc
      181 tctactatta ttgactttcc ctttcttttt atgtttaagt aataacttaa acttgattgg
      241 ggtgaccgag aatgaagaga acatttcagc cgcacaataa gagtagaaaa agaacccacg
      301 gctttctcgt aaggatgagg actgcatcag gaagaaatat tatcagacga agacgtgcga
      361 agggaagaaa acagctcacc gtgtgaaagc ggcaaaacca gtagatatca actataacac
      421 tgattttaat cccatggcaa acacattttt taaagatgag aggttagcta agggtgattt
      481 tcgaggcatt aagtggaaaa aatttgctga aacaccccac tttttactac tggtttacgg
      541 aaatgataaa ggtcaaaaaa aatttggagt tacgatacga aaaaaaactg ggggggcagt
      601 agtcaggaac cgtatgagaa gactggtgaa agaattttac aggctcaaca aagacctgtt
      661 cccggatcgg cataataatc ttctgaaaat caagcgggtt cctgaaagac taacatggac
      721 ggagacagac aaagaactgc acgcgttatt gcaggtacat accctttaaa aaaaatagca
      781 atctgcctgc tggatatata caaaacttgt atatctccct ttatacccac ccaatgcagg
      841 ttttatccca cctgttccag ctatatgcaa gaagcaattg agaaaaaagg gattttcagg
      901 ggtctggcgt gtggcatcat gcgaattctc cgatgtcacc ccttcagtcg tggcggttac
      961 gatccggtca aatagcggag gaatgtaatg gaaaaacgaa ccatgatagc gcttctatta
     1021 accattgtcc tgcttttttt cttccagatg tattttgcac ccaggcaacc tgtgcaagaa
     1081 gcgcctcagg ccaaacctga agagaaggca caaacaaaaa ccgatgcacc ttcacaacct
     1141 aaagtcactg gcagcgtacc cctgaccaaa actgaagcac caaaacctga agaaaccaag
     1201 gcaacccaac ggtttctcgt ggaaactccg ttgtttaaag tcactttcac cgatcttggc
     1261 ggtggtattg aaagtgttaa gctaaatcaa tacaaagaag cggtcaaagg agttggaggc
     1321 aaagaactcg ttcaagacat caaaccgtac agctataccc cgaaagtctc taaaaccaca
     1381 aacggttcaa cagtaaacga ccgcactatt tttaaaccag acaaaaaaag gatgagtata
     1441 caggataaac cagaaaccat cattttttcg ggaaccttga ctgatgggaa aaggatacga
     1501 aagatttata cgttctatcc ggataaatac actatagaca tgaagattga ggtagaggca
     1561 acagatgctg acaaggcgat ggtggatttc accgttatat cgggcacaaa tccaaattca
     1621 tacgttttta aaggtccttt catatataac gggaaaaaat tcgagcaaat cgacaagatt
     1681 gataaggata tcgcagtcga caagacatac acgtacgcag gacttgatga aggatttttc
     1741 gcttttatct ggttaccctc cgaagattca cattttcctc ttactattca taaggcagaa
     1801 aacaacgttc ctgtccttcg gatgtccatg gacaagggca tggcaacagg gaggctcttt
     1861 tttgggccaa agcagaccga tgttctaaag tcattggatg tacaggcgca gaaaatcatc
     1921 gattttgggt ggttcgacgt tattgccaaa cctctgatct gggccttgaa catgatgaac
     1981 agggtcaccc gtaactatgg aataaatatt attatactca ctatttttat aaaaataatc
     2041 ttctatcctc tcacggtaaa aagctccacc tccatgaaaa aaatgcagaa gcttcagccg
     2101 atgattgtga aattgaaaga aaaatataaa gatgacaaga caaagctaaa ccaggaaatg
     2161 atggcaattt ataaacgtga aggggttaac cccatgggcg gttgtctacc catgatcatc
     2221 cagatacccg tgttttttgc gctctacaaa gccctttccg gggccatcga actccgtcat
     2281 gcccattttt tatggtggat gaacgatctt tcagcaccag aagacctgtt tacacttagt
     2341 gttgccggat acgccctgcc cataagaatc cttcccctga tcatgggaat tacccaggtg
     2401 cttcagcaaa agatgacgcc taccacagca gatccaatgc aggaaaagat tatgcttctc
     2461 atgcccattg tgtttacgtt tttattctgg ggttttccat caggccttgt attgtactgg
     2521 ctcgttaaca atgtaatatc catagcccag caatactata ttaacaagaa agcaacctga
     2581 ggaggtaaca tagatgaatt ctatagaaat tgaaggaaaa acatatgaag aagcaatcaa
     2641 aaaagcttca gaagctttga atgttgaaga aaaggacctc gatgtcgatg ttaaggaagt
     2701 tgatacaaaa ggcatacttg gccttcttgg aagtaaaaag ataagaataa cagcgaggat
     2761 caaaggcaca gcctcccctg ttgcagagga taaaccagtt tcagagaata caatagttcc
     2821 agaggatgca ccagtttcag aggatacacc tgaacagtat ggaacaaaat tcctcacaga
     2881 tgtggcaaca attacaggat tacattttaa catcaaggtt agcaaaatca gcgaaaagct
     2941 cgaccggctt cttttcatga tacaatgtga caacggggat gtttttgtcg gcaaagatgg
     3001 tgagatcctc gaagcgcttc aatacctttt gagacttgca attgcaaaac attacaaaca
     3061 atctgcaaaa atactcgttg acataaacgg tttcagagaa aagaggaaaa aggcactcac
     3121 tatcatggca aagagacttg cagaccgcgt gcggaggacc ggaaggcgcc agacaacaga
     3181 tccattgaac ccatatgaga ggagaattat ccatacactc ttcaaacaca ataagaatat
     3241 cgcgacaaag agcgagggag atggccacat aaagaaggtt atcatctctc cctcaagcaa
     3301 cagacctgcc cgtgccggct acaggcatgg aaaccgttga cactatttgc gccatttcga
     3361 ccccgcctgg tgagggcgga atcggcatca tcaggataag cggcccaaaa gcacacgaac
     3421 tgttgttcaa gatatttcgt cctgctaaaa caaaaaaaaa ctatctgcct cgtaccctct
     3481 atcttggtaa gatcacgaac agtgatagta atgagaagat tgatgaggtt tatggcgtcc
     3541 ttctgaaagc accaaaaacc tatacacggg aagacatggc agaaattcac tctcacggag
     3601 gatttgcatc ccaacaggcc atactctctc tcctaatccg gtgtggtgcg cgtcttgcgg
     3661 aaccaggcga gtttacaaaa cgggcatttc tcaatggtag gattgacctc ctccaggcag
     3721 aagccgtgct cgatatcatt cagagcgaaa cgaatgaaga gttgcgatgt gctgtggaac
     3781 atcttgaagg caagctttct tccaagataa atacaattaa gaacaatgtc aaaggagtgc
     3841 ttgtggaaat agaggctctt attgactttc ctgatgaaga gatcgatgtt gatatggaac
     3901 aatgcctgac tattctccgc agaacccata aaaacgtaga aaaactcatc aaatcctatt
     3961 atgaaggaag agcgatccag catggcctgg aagcccttat tatcggaaaa gcaaatgtag
     4021 gaaaatccag tctattaaac gccttgctgc ttaaagaaaa ggcaatagtc actcctctgc
     4081 cgggcaccac ccgcgacctt atcgaagaca tactccatat aaaaggaatc aaggtaagaa
     4141 ttgtagacac tgccggcctc cgaaaaccaa aagatattgt tgagcaggag gggattgatc
     4201 gagtaaaaaa taaggttcct atgacagatc taataatctg ggttctcgat ggctctgacg
     4261 cttacacatc agaagacgaa gacatctata cagctatagc gggaacaaac atactggtcg
     4321 cgattaataa gattgatctt ccccaaaaac tcgataatac aaaaatattg tcgcggggtc
     4381 tggagtttta taacatatct gccctccgtg gttctggtct tgaagaactg aaaaacggca
     4441 tttacagaaa actcatgggt aagaagggta aacgggctgg cttactgatc acaaatgtcc
     4501 gccaccgaga agtacttcag aaggtttatg aggcaattgg aagggccata aattgtcagg
     4561 agaaccagga gcctgtagag tttgcagcct ttgaactcag ggaggctctc tttaatcttg
     4621 gagaaatcac cggagaaacc tgccccgaag atattctcca tgatatcttc tcccgattct
     4681 gtataggtaa ataatagctg gaaaaaaata aaatgctgct gatagccaat tctggcttta
     4741 atggatacct gcagcctctc caacgcacag agcctctcta ccagaagttt gcatcatttg
     4801 tgaaaagatt tttctttatt atccttgaca agaatttgaa tcaatgctaa ttaaatcgat
     4861 aaatggttag aaattcgttt taaaatattc tgtgagtttt cagttaggac gttggaatgt
     4921 gaccagttgg tagttcactt catgcgcact ttcttgtagg agcttgagga ggcccgtaca
     4981 tgaacgaaca tgaaagaacc attaaatctg gcattaacga tccgcaagta ggaactgtga
     5041 catctgttcg gggaagtgtt gtagacgcat cctttccggg caaacttcca cccctccata
     5101 atctgctccg caccggcgac gacggtcgaa ttgtcattga ggtcatcacc cacctcaatt
     5161 cagaaatggt acgtggcatt gcactggtac ccacacaggg cctcgcttac ggttcatcaa
     5221 tcatcgatac tggcaatgca ttgagggtac ctgttggtaa acggcttctg ggcagagtct
     5281 tcaatgtttt tggcgaaacc attgacaaaa tggaccctgt ttcaggcggt gagtggcggt
     5341 cgattcaccg cgattcaata cccttaagcc agcaatcaac cgtttcagaa atattcgaaa
     5401 caggcataaa agcagtggac gtgctcgccc ctctcgaacg gggcggtaaa gccggcctat
     5461 tcggtggtgc aggcgtgggc aaaactgtgc tcatcacaga aatgatccat aacatgtttg
     5521 gccgccatga aggggtaagc ctcttttgcg gaattggcga acgttgtcga gaaggcgaag
     5581 agttataccg tgaattgcgt gacgcaggtg ttctggacca taccgtcatg gtattcggtc
     5641 agatgaatga gcctcccggg gccagattcc gcgttggtca tgcagccatg acaatggcag
     5701 agtatttccg ggatgatatg cagcaggacg ttcttctgct cattgacaat atatttcgtt
     5761 ttatccaggc cggaatggag gtatcggggc ttatggggta tttgccctca cgtttgggct
     5821 atcagcccac cctagggacg gagattgcaa aactcgagga aaggatttgc aatactgcca
     5881 ccggagctat aacatcggtg caggccgttt acgtccctgc tgatgatttc accgatccat
     5941 cagcagttca tacgttttct cacctctctg catcaatagt gctttcacgc aaaagggcaa
     6001 gcgagggcct tctcccggca atcgacccac ttcagtcagg ttcacgaatg ctcgtacccc
     6061 atcttgtagg aaaaaaacat tatcatgttg cccaggaaat acggaaaact cttgctactt
     6121 atgaagacct caaggatata atcgccatgc tcggtattga ggagctttca aaagaagacc
     6181 gcaagactgt ctatcgtgca cggcgccttg agcgctttct cactcaacca ttttttgtga
     6241 cagaacagtt taccggatta aaaggaaaga cggtcagccg tgaagatgcc atagagggct
     6301 gtgagcgaat actcaacgat gagttttcgg agtatcccga acgtgctgtt tatatgatcg
     6361 ggactataca tgaggccaaa attcatgaat cttaaaatcc ttctgccggc tggaatttta
     6421 ctaaatgagc aggtaaacaa ggttattgcc gaggcagtga atggttcatt ttgccttctc
     6481 ccgcgccaca ttgatttcct cgcagccctt gctcctggta tcctgtcctt tgtctctgcc
     6541 gaaggcaaag aggaattcct ggctgtggat gaaggcgtgc ttgtcaaggc aggaccggaa
     6601 gtgctcgttt cttcaacacg ggccgtacga gggggagagt tgggccaact caaagataca
     6661 gtcgaaaggg tgtttcgaca tattgatgat caggaaaaat cagcccgttc aaccatggca
     6721 aagctggagg ctgatttggt gcggggattc atggaactgg gagagcgtgg ctacaagtag
     6781 catactgaga gatacagaac ggatgagcga agattatctt tccaaaaaaa taggaaaaaa
     6841 agaagcccgc aagctgaaag cgaggagcga gcggcgttac agcgctcttg tgaagggcct
     6901 tcgcatgaca ggtcttgtgg gatggacagt ggccacacca acccttattg gggtggccat
     6961 aggtaaatgg cttgacaggg catggccggc taatttctcc tggaccctca cccttctcat
     7021 ggccggtctt atccttggct gttttcatgc gtggtactgg ctcaaacggg agagcaagaa
     7081 tgactgaatc gatcgctttt aatgtctcaa ttgcatttgc agcaggtctt ttgctgggtc
     7141 tgttttactt tggttccctc tggatgaccg tgcagggcct tgtcaaggta aagaaaccgg
     7201 ctctgctctc ttttgtaagc ttttttgtca gaacagggtt aacactcacc ggatttttct
     7261 tcgttatgtc cggacactgg gaacgagcac tggcctgcat gtttggtttt ctcgtaatgc
     7321 ggaagatact gacacaccgt tacggcttgg accatcaaac cctccagagg aggaagggat
     7381 agcttaaggt atggagatac tacacctcga ccagatcagt cctgatgcag ttgtcctttg
     7441 gaagtggaaa ttctttaccc ttaacgcaac aatactgtac acctggataa tcatggcaat
     7501 tttaacggtc gggtcatggc ttgccacccg taatctctcc acagaacctg aaacatcgcg
     7561 ctggcagagc cttcttgaag ttgttgtggt tgggatgcgc aatcagattg gagaaatctg
     7621 tcagcaggac gctggtcgct atctgccgtt tattggaacg ctcttcctat ttatagctat
     7681 ctcaaatctt ctcggtttcg tgccaggcta tgctccccct accagctcgc tttcaacaac
     7741 agcggctctg gcggtttgtg tgttcgttgc ggttcctttg tacggcatgt gggacagggg
     7801 ccctatcggc tacctgaagc attttgcaga gccctcccta ttcatcatgc cttttcatat
     7861 attaggcgaa gtttcccgta ccgttgccct cgccgtccgt cttttcggca acatcatgag
     7921 cggcagcaag gttgtaggaa tactccttgc aattacacca ctgcttttcc ctattgtaat
     7981 gcaggtgctc gaattgttga taggtcttat ccaggcatac attttcgcca ttctttccac
     8041 agtgtatatt gcatcagccc ttaaagccca tgaggagaaa gaaagctttg cacacagttc
     8101 cggtagtttg agcggttcaa ccactgtaag cggatgaacg aacaaaaata taggagacgg
     8161 tttaaccaaa ttataataag gaggagatga tatggatgtt aagaccctgg tagggatgat
     8221 ctcgataatt attgctggaa tgacgatgtt tgtgggttcc ataagtcctg ccctgggcat
     8281 ggcccgtgca gtggcacagg cgttgagctc catagcccag cagccggacg aggcgaacac
     8341 tattactaga accctcttca taggtctggc aatgatcgag tccttggcta tttactgttt
     8401 tgtcatttcc atgattctga tttttgccaa tccgttctgg cgttatgtga catctcaata
     8461 aggaactcgc catgctgatt gactggttta cagtcgttgc tcaaattatc aatttcctta
     8521 ttctcgtatt cctcctaaag cattttctct atggcaggat catcaacgct atggaccaga
     8581 gggaggctcg tataacatcc cgtcttgaag aggcggaaac tcagagaaaa gaagccgaga
     8641 atgaggcagc gatgttcaaa ctcaagaacc agtacatcga gtctcaacgg gaagagatga
     8701 tctcgagtgc aaaggaagaa tctgagtcct ttaaaaaaga ccttatgcaa aaagcacggg
     8761 aagagattga cagtatccag gcacggtggc atgagactgt agaaaaacaa aaaattgctt
     8821 tcctcaagga tcttgggcaa cgagcaacaa aagaggtctt tgccgtaacc cgtcaagtgc
     8881 ttaaagacct tgcagataag gacctcgaac agcagatcgt tgatgtcttc atacggcgcg
     8941 ttgaaaaact cagccaggag gaacggcgtc tcatttcaga atcagttaat ggttcgggaa
     9001 taattcttat taccagtgcc tttcaaatac ctgatgaggg gcaggtaaaa attctcaata
     9061 caatcaaaga atatattaat aatgaatgtc agatagacta tcagattgca tctgatgtca
     9121 tttccggcat cgaactccgt accgtgggtc ataagatcgc ctggagtttt gataattatc
     9181 tggcgtcttt agaaacgaat gttttagaag ccatggaatt ggggtcgaga agtgaggggg
     9241 cagaatgagc gctgatgaat tacatacagt tcttgatagc acgtttagtt atatcgaaca
     9301 tgagttggag agcttcaaac ctgatgttaa ggctgacgag gttggtacag ttaaatccgt
     9361 aggccagggt gtggcacagg ttgaaggtct cccccgtgca aaatcagagg aacttctccg
     9421 ttttacagga aatcttctgg gcaccgtctt taacgttgac ccttcaggta tgggcgtggt
     9481 cctcctcgat cagagcgcag acctgaaagc aggatctgag gtatatccca caggaagggt
     9541 gctcgatgtt ccggttactg aagcactcct gggcagggta atagatgcaa tggggcgccc
     9601 cctggacaat caaggcccaa ttcagagcac tgaacgcctt cccattgagc gggaagcacc
     9661 ggcaataatg gaccgggccc ctgtggttgt gcctcttcag acaggactga aggttgttga
     9721 tgctctcatc cctataggcc gtggccagcg agaactgatt cttggagatc gccagacagg
     9781 aaaaacagcg atcgtggtcg acaccattat taatcagaag aatgaagacg tcatctgcat
     9841 ttactgtgct atcggaaaac agagtgctgc tgtggcgcgg ttcatagctg acctaaagaa
     9901 gtacggggcc atggaatatt ccatggttgt gcttgccaca ggggaagata cgccaggtct
     9961 gcaatttgtt gctccctatg cggcaactac catgggtgag tattttatgc gaaagggtca
    10021 tgatgttctg gtcatatacg atgatcttac ggcccatgct cgagcttacc gcgagttatc
    10081 gcttcttcta cgtcgtcccc ctggcagaga agccttccct ggagacatat tctacatcca
    10141 ttcccggtta ctcgagcgat cgacacacct caaggaagag cttggaggcg gttccctcac
    10201 tgccttgcct attgttgaaa cagaggcgca gaacatgtca gcatatatac ccacgaacct
    10261 tatctcaata acagacgggc agatctatct ctcaccagac ctgttccaga agggaatcct
    10321 tcctgcagtt gatgttggaa aatccgtgtc aagggtcggc ggcaaaactc agctggcagc
    10381 ttaccgggca gtggcaggag atcttcgcct ctcttactct cagtttgaag agctcgaagc
    10441 tttcgcacgg ttcgggacac gccttgacga agcaacgctc aaaacccttg aaagaggcca
    10501 tcgtgtgcgt gaagttctca agcagtcaca atacgagccc ctctccgtcg ctgaacagat
    10561 cgcctgtttc ctggcagtga cgaacggcgt tttcgataat attgagattg acgcggtaga
    10621 tgaggcgcag accacaatcc gtaagaacct cgttcagcag ttacctgatg tttgtcaccg
    10681 catagagaag ggtgaggagt tgaccgctga agacaaagaa tctattctga agatggcaaa
    10741 agacaccgta accttccatt acggtatcgt ttagtatgcc ttctattgaa acactcaaac
    10801 ggagaattcg cactgccgga gatttacggt ctcttgtaag aactatgaaa gccctcgctg
    10861 ctgttagcat tcgtcagtac gaaagggctg tcgaatcact tgccgactat aacaggactg
    10921 ttgaaatggg ttttcagata ctcatgaggg aaaaggagac ttcccttctt agggagcggg
    10981 ggactaaaaa atgtctcgga gcagttgttt ttggatctga ccttgggatg tgtggccagt
    11041 tcaatgaaca gattgccgcc tatgccctcg aagaaatgaa aaaactcggg tgtgaacctt
    11101 cagacagatc atgcgtcgtg gtgggtgcgc gagtaacagg ctaccttgaa gatggtaatc
    11161 aaaaagtaga taaagaactc tcggtgcccg gttccatgtc cggtattaca ctgaccgttc
    11221 aggaaatcaa cgaaatcctg tcggactggt ggatgagcaa aaaaatcaat cagctctatc
    11281 ttttctataa caaccatata tcagggtcac agtatcgtcc tcacagtgtc agactcttgc
    11341 cccttgatct tgaatggctt caggatataa agatgcgaaa gtggccaaca aaagtcctgc
    11401 ccatgtacac attggaatgg caaaaacttt ttcctcttat cgtacgtcaa tatgtttttg
    11461 tctctttatt tcaggcaact gcagaatcac tcgcgagcga gaatgcaagt cgtcttgctg
    11521 ccatgcaagg agccgagaag aacattgatg acgttctttc agggcttaaa cttcagttcc
    11581 accaggaacg acagatggct ataaccgatg aacttcttga cattgtggcc ggcttcgaag
    11641 cattagcaga gccagaatag tacgaaaagc agcttatagc aatcatagtt gatgcttcct
    11701 cagcagcaca ctatttttct tgcaaaacat caaagattga aggacaatgt ttgatgctga
    11761 actaaatgaa acttcatgaa aaagaccacc aaagataaca atcaaattca ggcaaaaaac
    11821 tctgaacata ttcaggaaat cctatcacag aacctcaaca tatatcgtct cattgcagag
    11881 aacatgtatg atgccattta cacactggat accaatgtta atttcacctt cgtcaatgat
    11941 gttgccctga agagatctgg ttatacaagg aaatggttca tcgggagaag cttccttaat
    12001 tttgtcattc cggaaaacaa aaacatggtt caaaaaagtt tcaaatctgt aatgatgggt
    12061 aaggttgtgc cagcatacga gctgagttac ataaattcta aaggtaaatc tttatggata
    12121 gaaataaatg caacccctct tgttgataat gggaatatca ttggagtact ggttgtctcc
    12181 cgggatataa ctgagagaaa aaggaatgag gaagaactga agctttatcg taacaatctt
    12241 gagtccctca tcgaaggccg cactgctgag ctaagaattg caaacgaaca attacagatt
    12301 gaaatcgctc agcacaaaaa gaccgaagaa gccctgcgca gttcagaagc ctattaccgc
    12361 accatcttcc ataacacagg gactgccatc ataatcatgg aagatgacac aaccatatct
    12421 ttgtccaatg cggaatgtga aaaatttgtc ggatatcgag cctctgacct ggagggcaaa
    12481 agaagagctg ttgagttcgt ggccagacag gatctggaga ggatactgag ttataatgaa
    12541 ctaagaaggc aggatcccga aaaagctccc agaagctatg aactgaagat tgttgatcga
    12601 catggcaacg tcagagatgt cctcatcacg atcgcatcaa ttcctcatac aaacaagaca
    12661 atcgcatcat ttcttgatat cactgaacaa aagaaaatgg aagcagcact caaggcatca
    12721 gaggaaaaat atcggaacat tttcgaaaac gctgtggaag gtatttttca gataggaatc
    12781 aatggagaaa ttctaagtgc aaatccttct ttttcacacc tattcggata taaatcacct
    12841 caagaaatca tgagggcagt caaggatatc cgttacgaaa tctatgcaga tgaaagtcag
    12901 agaaacgaat tacggcgcct tctcgacaag cagggattcg ttcgcaactt tgaggtcata
    12961 tgccgaagga aagatggtca aagaatatgg atcagcgtca atatcagagc tgtaaaggac
    13021 aacaatagca aattcctttt ctacgaaggg acactcgtag acatcactga acggaagatg
    13081 atccaggatg atcttgaaaa gaagtcgaga agccttgagg agacaaatgc tgcgctcagg
    13141 gttctgctca aacagcgaga ggaggacaag actgaccttg aggaaaaggt cttgcataat
    13201 attaaggaac tggttcttcc ctacatcgat aagcttcgag cgggacaaca aaatagagat
    13261 acggttatca tcgacattgt ggaaagcaac ttgaacgaga ttctttcccc cttcatcaag
    13321 agtatggcct ccaaatatgc caattttacc cccaaggaaa tccagatagc agatctcatg
    13381 aaaaaaggga aatcaactaa ggaaatatca acgatcctta acctttcacc caggacaata
    13441 gacattcatc gttacaatat cagaagaaaa ctcaatatca ataaaaagaa agtcaatctt
    13501 cagtcttatc ttttaactct tacatagtta atacttattt tatctagata ttaattacgt
    13561 taatcattga tattctcttt ttcatttttt cagccataat cgtagcatga caacattgac
    13621 acaatatgaa agcagtccgg taaggcaatt aaacatcctg cgttgctggc gcattcgagt
    13681 tcttttgctt gtcctcataa tcttcgcaac tctctgtaac ggctgttcag ttaagaaaat
    13741 agccatgaac catctcggtg acgccttagc aaaaagtgga tctaccttct cttctgataa
    13801 tgatcctgac atgatcaagg cagctgcacc ttttagtctc aagctcatgg aaagtgttct
    13861 tgcagaaaca cccgaacata cagggctcct tcttgctctc tccagcggat ttacccagta
    13921 tgcattcgcc tttgttgtcc aggaggctga cgaaactgaa gagaataatc taatcagggc
    13981 tatggagaca cgtgcacggg cattacgcct ttatctgcgg gcaagagatt atggactgag
    14041 agcacttgat atccgccatc ctggttttgc agaagcactc cacggtgaac cggaaaaagt
    14101 tgttcaagat ttaaaaaagg gtgatgtacc ctatgcatac tggacagcag tttcctgggc
    14161 gggggccatt tcaatggcaa aaaacaacgc ggatctcatt gccgatcttc ccaaagtaga
    14221 ggctcttatt gataaggccc ttgcgctcga tgaatccttc gataacggag ctattcattc
    14281 atttcttatc gcctatgaca tgaatcgtgc atctggcact ggtaatccgg aggaaaacgc
    14341 acgaaagcat ttttcgagag cagttgaatt gagcgacggc aagcaggcag gcccatatgt
    14401 aaccatggcg gaatcagtgt cccttccaaa gcaagataag aaagagttca tactttttct
    14461 cgaacgagcg cttgcaataa atgctgacgc aaaaccagag tggagactgg caaatgttat
    14521 tatgcaaaaa aaagcccgct ggctgcttca gaggactgac aagctttttg tcaactgaag
    14581 tgggggattt caattgtgag gaggaaaaaa catgaaacat ataataagag tatttctcct
    14641 ggtattaatc ctttctttgg catatttcct ggtacagccg tctgaggtct catgccagga
    14701 caaaccatta gaaatacgcc ttgtcaccct tgcgcccatg ggaacaagcc cccacgtagc
    14761 actgctcaag atgggagaaa agtggcaga
//
LOCUS       DAOOMC010000074        14632 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdus sp. isolate AMR_MDS_2685
            NODE_4400_length_14632_cov_6.646292, whole genome shotgun sequence.
ACCESSION   DAOOMC010000074 DAOOMC010000000
VERSION     DAOOMC010000074.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864760
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdus sp. (wastewater metagenome)
  ORGANISM  Syntrophorhabdus sp.
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae; Syntrophorhabdus.
REFERENCE   1  (bases 1 to 14632)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14632)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,729
            Genes (coding)                    :: 2,708
            CDSs (with protein)               :: 2,708
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 7 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14632
                     /organism="Syntrophorhabdus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4400_length_14632_cov_6.646292"
                     /isolate="AMR_MDS_2685"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2699756"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..790)
                     /locus_tag="PK125_08595"
     CDS             complement(<1..790)
                     /locus_tag="PK125_08595"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HPB38206.1"
                     /translation="MNKEGKIRVVIFYSVMAHYVTGVLKALKKCGKGLDIDVVFWDKR
                     SPNSNLYKVEELESVRCHARSLLDNKALLDLLHTRNPHITYISGWMDKGYLQAIKRYR
                     SEGGRTQVVCGIDDQWKGTLRQRLGQIYFRFFYRKIFDFMWVSGKPQYHYAQRFGYDH
                     ERIISNMLSADTSVFDQKHFFSRRFIFVGRFVPVKGLDLLLDAYLSLPAETRSEWPLI
                     LIGDGGIKHDIEKRASERITVKPFMQPDALREELMHGGVACIPSH"
     gene            complement(886..2019)
                     /locus_tag="PK125_08600"
     CDS             complement(886..2019)
                     /locus_tag="PK125_08600"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HPB38207.1"
                     /translation="MKVVHLIHSIAEQASGPSYSIRRLCESLIENKQEVILATLDWDV
                     MTETPGFLKEFPLTLGRRRPGLSSSMRLWLTKQAEMKKVDLFHSHSLWMMPNIYPGNI
                     ARKNNIPYIISPRGTFAEYAMASGSKIKHVFWPLIQRPAVNAANCFHATAMSEFEDIR
                     RLRFSQPIAIIPNGIDMPQHKETTLGNTRTLLFLGRIHLEKGVDNLLRAWQVVSRGFP
                     EWQLKIVGPDSGGYLSKMKALKEKLRLKRIEFVGALYGEKKIEAYRNAELFVLPSPSE
                     NFGISVAEALSVGIPVIVTKGTPWRNLESLGAGWWIDIGVDPLVACLEVALSKPRDIL
                     KAMGIRGREWIRIEFSWTEVTRKMIETYQWILRGGSKPDWIFE"
     gene            complement(2188..3432)
                     /locus_tag="PK125_08605"
     CDS             complement(2188..3432)
                     /locus_tag="PK125_08605"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HPB38208.1"
                     /translation="MGTAQAIQALSLLVLIKILNPAIYPNEGPISLLGWVVIALAGAR
                     IFIDNLRVTWKIQLVLLWLLLFSAVILLESIFFSYYRVVSTFKIASFAYLALAVLIGF
                     KATANKSVDWTSWFLGIWIAVTVLSAPTFFFPRIGFFLDGMGFQGLLNHPQTFAIFLV
                     PMVAWLMGRLLFLPLKGHYWLYSILTLAWASLFLTRGRTALVAVIMGFTVVILIGLIK
                     RNEWRKLLRRSIFSPIFLVLIISYLAIALFRPPTVLEAVRSFIFKDLPDMGISESFKK
                     SRGFLIVESMDNFIQHKWFGIGFGVSDSIFIPFDPVVEPLTGLPLAAPTEKPMLPVAL
                     LEETGIIGTAFFTLFLFTLMKLLISNGNLILSLVFLTCLFLNIGEMIFFSVGGLGLYI
                     WLIMGWATYSRLERHKAIKPTT"
     gene            complement(3586..4848)
                     /locus_tag="PK125_08610"
     CDS             complement(3586..4848)
                     /locus_tag="PK125_08610"
                     /inference="COORDINATES: protein motif:HMM:NF014051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HPB38209.1"
                     /translation="MKTLLTNAGWSAAAHLLERGSLVLVSIILSRMLATEGFAAYGYF
                     QMTVSMLAAYAAMGLGVTASKFFAESDQQSGDAMPPLGTLWALSVLSGLLMALVVLLV
                     PSSWFESGLNIPSWLLAVGVFVLSLGVIPGGGILGLEKYVVATWASAASSVIIVVGAL
                     VAGRLNSPQSAMSVLIFASLVQTAINSWLVTKVIGLRPLLTGLWIGGFQIRRIFRIAA
                     PMATISLMTSSATWFVGRILLSGPSGHWQFSLYVIGLQWLAIVLYLPGMITRVIFPQL
                     VRVRLIESLNASLQTSVILHQGMMLASITAFVICCIAVVGSPWLMCLYGSHYTSYHWL
                     IMWFMFAALPQAPTGVLGNGIIADNGQWAWLFIIIVWFVLFIVFTWSQKNSGAIGAAL
                     SYGLASLIQAVFAYLVAHRRGLIRVGNR"
     gene            complement(4845..5213)
                     /locus_tag="PK125_08615"
     CDS             complement(4845..5213)
                     /locus_tag="PK125_08615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB38210.1"
                     /translation="MLLPLARYYNNDNFALDSKIFAMKITRIYEKMLKTLFAQFPAVV
                     VTFIFRRCPIWFWRTARGDEVDLIIEKDGRFTTTECKYAKAISSSGLKGLNAFTETYG
                     HENNRNCYVASRTATEHPWS"
     gene            complement(5334..5582)
                     /locus_tag="PK125_08620"
     CDS             complement(5334..5582)
                     /locus_tag="PK125_08620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB38211.1"
                     /translation="MNGTTEKIAASICNKFRKKNNQERMRMGFAMFDTAKTIMLASRP
                     NLSVTEKRKMLFLRLYGNELDSQIIKKVLAHLESLLVQ"
     gene            complement(5712..6122)
                     /locus_tag="PK125_08625"
     CDS             complement(5712..6122)
                     /locus_tag="PK125_08625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB38212.1"
                     /translation="MEQLEVLRKVVEKLCDLDIEYMLSGSVAMNFYGQPRMTRHIDII
                     ISIKETQIRRFIDAFKEEFYIDSEMVLNEVSGKGMFNVILNDYTVKVDFILRKDTKYD
                     INAFERRRLVNVGDFQICLISPEDLVPNKLLWAR"
     gene            complement(6122..7225)
                     /locus_tag="PK125_08630"
     CDS             complement(6122..7225)
                     /locus_tag="PK125_08630"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase"
                     /protein_id="HPB38213.1"
                     /translation="MKGRILVTGAAGFIGFHLSKRLLEEGRSVVGIDNLNDYYDVNLK
                     KARLAILKKYDNFTFHKINIANRRAMESLFNGLLKTQATHERVPRLKIKEPPITCHPS
                     RVTGTFDTVVHLAAQAGVRYSITNPYAYIDSNIVGFMNILEGCRHHKIKHLVYASSSS
                     VYGANTKMPFSIHHNIDHPVSLYAATKKANELMAHTYSSLYNIPTTGLRFFTVYGPWG
                     RPDMALFLFTRTILEGKTIDVFNRGNMQRDFTYIDDIIEGVMRVMKKIPRPNKSWSGD
                     NPDPGTSYAPYRVYNIGNNNPVELMTFIKTIEKTLGKKAKKNLLPLQAGDVPATYADV
                     DDLIHDVGFKPATPINEGIKKFVDWYLMYYQES"
     gene            complement(7257..8642)
                     /locus_tag="PK125_08635"
     CDS             complement(7257..8642)
                     /locus_tag="PK125_08635"
                     /inference="COORDINATES: protein motif:HMM:TIGR03026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HPB38214.1"
                     /translation="MDLVRKIENRSARVGVIGLGYVGLPLIIEFVKQGFSVTGFDIDQ
                     DKVDFLKAGKTYIKHIDIAPLTSKGKRAGGRGLKTNSESSAVAPYSKSERLVTSGLQP
                     VFNATTDFSMLKNMDCILICVPTPLNKNREPDMTYVFGTTKTIAQSLKKGQLIILEST
                     TYPGTTDEDMRAILEKTGFKAGKDFYLAFSPEREDPANKDFSLRTVPKVVGGYTRNCL
                     MAAKALYDTIVIKTVPVSSTKVAEATKLLENIYRSVNIALVNELKILFDRMGINVWEV
                     IEAAKTKPFGFQPFYPGPGLGGHCIPLDPFYLSWKAHEYEFSTKFIELAGEINTSMPD
                     YVVSKVMKALNDVGKPLKGAKILILGLAYKANVDDDRESPSYRLIEKLEKLGAHVSYN
                     DPYIPVIRKSREYARYAGRKSAKINGNYDCILIATAHDEYREIDFKSFGIPIVDTRAI
                     VKGKSKFFYSA"
     gene            8948..9163
                     /locus_tag="PK125_08640"
     CDS             8948..9163
                     /locus_tag="PK125_08640"
                     /inference="COORDINATES: protein motif:HMM:NF018961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dodecin family protein"
                     /protein_id="HPB38215.1"
                     /translation="MRSDGRVARITEVVAGSPKNFDDAVLVGFKRAARTLRGITGLRV
                     KDMRCRVEEGKIIEYRVTLEVIFILES"
     gene            complement(9147..10184)
                     /locus_tag="PK125_08645"
     CDS             complement(9147..10184)
                     /locus_tag="PK125_08645"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HPB38216.1"
                     /translation="MNVTECRTSNAGQNLSIVIITRNTKELLRNLLKSIEADKLLRPF
                     LKEVIIVDNYSTDGSNTMVDSEFPWVFLVKNEYNLGFAAAANVGIARAEGSYIFLLNS
                     DTLLIEGEVAKMVQLMEENRDVGICGPQLVHEDMRHQRSYANIPSLLFEIVPRSLMEI
                     LFPARHSTKSLQEDSASHIPSPLAHRPAPAQQCFDVPSLIGAAIILQKKLLTDLGGLD
                     ERFFFFLEETDLCIRAREIGARVVFLPHVRVIHLQGRTVRKQWIKGRIQYNISLYKFI
                     RKHHTIFYYRMFQGVRAMKSFVVPLVLTVLFPVLLHRRTRRTYTYYMKLLLWHLRGCP
                     DNAGLFISSRG"
     gene            complement(10310..12106)
                     /locus_tag="PK125_08650"
     CDS             complement(10310..12106)
                     /locus_tag="PK125_08650"
                     /inference="COORDINATES: protein motif:HMM:NF013974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HPB38217.1"
                     /translation="MREVMIGNFAIARGLVEAGLELAAAYPGTPSSEILPGIIEFKNR
                     EKLAIHAEWSTNERCALEVAFGGALAGRKAACMMKQVGLNVAFPPLLHGYAQPLKGGL
                     VIVSCDDPGPQSSQTEQDTRLLAAHFGIPVFDPASPGEAGDIAYYAMKYSCEHKVPVI
                     VRSTHRVSHARESIELFVPGSRKVKLDEGLKLKGKGRIGIVTSGMTCSITMDILDELN
                     LGDKIGLYKVSRVFPLGTEVIGFVNQQEKTLVLEETDVALEALLDHGFKVLGRRSGHV
                     PSAGELTYDNVRDIICRFANDHGISVPEFNADTSIEDALKEIQIAPRPPKLCAGCSHR
                     ASFYAMKSAFPDAVFPGDIGCYTLGISMGAVDTCLDMGGGVTFASGFYDTFAQDGTLK
                     PILASVGDSTFFHACLTPLYDSVLKKRRFVLVIMDNSTTAMTGLQPTPQSGITADGTS
                     TYSIKIENIIKGFGIEFLRIVDPYDIPLLLRTIKEAYEYLETESAGPAVIIARRECLL
                     FSKIREEDTRDMARLVAECTGCKICIKTFDCPGSYFDEEKKKICIDEGLCIRCGMCYY
                     ACPTSDEGKSLKELKKVRNEKKRREGREDSKK"
     gene            complement(12131..12712)
                     /locus_tag="PK125_08655"
     CDS             complement(12131..12712)
                     /locus_tag="PK125_08655"
                     /inference="COORDINATES: protein motif:HMM:NF013707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate oxidoreductase subunit beta"
                     /protein_id="HPB38218.1"
                     /translation="MNLQIVCAGIGGRGVLLASTIIIETAIKAGLKAMASDEYGMSQR
                     GGSVVSLVKVGNFASPLVGRESANILLSFEESEFYRNLPFLKTGGVTLVNTSRQLLSA
                     NIERLLKKRRVQYFLLDADAIAMQKGMIQSSNMAMLGFFSAFDITPFSCDNIKETIRQ
                     KVKARLVEKNLEVFQAGYESAQSFQLSADSKNQ"
     gene            complement(12905..14137)
                     /locus_tag="PK125_08660"
     CDS             complement(12905..14137)
                     /locus_tag="PK125_08660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB38219.1"
                     /translation="MYDQVCRVLAENGTRLLYTANRAAGGVVKMLESEYASLRGETSL
                     NEKIAFELALTGAYSSKRTACLFSTEGLYEALDPLMSSAYTGVIGGFVIVCVKETEEE
                     ITPLGLFSKLPLVVTESQDEFKKAIGFGYEISEKYEIPVIVQVAPDVAAQTEGKSSSS
                     IQNSEFKINNSVFNKNPGRWAATPKFRYELHRALNEKIEKIRYEFETYEGNRKRIKSK
                     TGVITNGHTNLDFFDEDSSLLFISTVHPLPVKLVNDFIAGMEEVFIAEGPYSAIELQI
                     QDRSKIVSAKVGDIPERKKPQETMYGFDVVRDTLGPASSINMAHGIKKTEPEKKILAI
                     TFEDFFLHSGMPAFVNTLYNGSAYIILILGDKKEEEIKKILAGFGFNNVFRIDNFSEI
                     ERFKDTEDMTVLFCRGII"
     gene            complement(14218..>14632)
                     /locus_tag="PK125_08665"
     CDS             complement(14218..>14632)
                     /locus_tag="PK125_08665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB38220.1"
                     /translation="TTPLLPMNSIGHQFVPAPIHAGGLRYHGMAPLVSRLLQDGLIEA
                     KAYAQLETFQAGIHFARTEGFIPAPETDHAIACVIDEAVKAKEEGKEKVILMNWSGHG
                     VIDLASYDAYLSGKLHDYIMPDEDIEQLVCELKRC"
BASE COUNT         4113 a         3421 c         2932 g         4166 t
ORIGIN      
        1 catgactcgg tatgcacgca acaccaccat gcatcaattc ttcccttaat gcatctggct
       61 gcataaacgg ttttaccgta atgcgttcgg acgccctctt ctcaatatca tgcttgatcc
      121 cgccgtcacc aatcaaaatt aatggccatt ctgatctggt ttctgccggc aaagatagat
      181 atgcgtctaa taacagatcg agtcctttaa cgggaacgaa acgaccgaca aaaatgaaac
      241 gtctcgaaaa aaaatgcttt tggtcaaaaa cggaagtgtc tgccgatagc atgtttgata
      301 ttattctttc atggtcataa ccgaatcgct gggcatagtg atactggggt tttccggaaa
      361 cccacataaa gtcaaagatt tttcgataaa agaaacggaa gtagatttgt ccaaggcgct
      421 gtcgcagagt gcctttccat tggtcatcga taccacagac aacttgagtc ctgccgcctt
      481 cggaacgata ccgtttaata gcctgcaaat atcctttgtc catccaacct gatatgtaag
      541 tgatatgtgg atttcttgta tgcaataggt caagtaaagc tttattatcc aacaatgatc
      601 gtgcatggca cctcacgctc tccaattctt caactttgta aaggttacta ttgggacttc
      661 tcttatccca aaaaacaaca tcaatatcta aacctttccc acactttttt aatgccttca
      721 gaacacccgt cacataatgc gccataacag aataaaaaat tacaactctt atctttccct
      781 ctttattcac tatctacgac ctaacaaata ttattacgtt taggaatctt gctcggttat
      841 gtcacaattg tggagtttcg ctgatttcac atggcaaacc cattattact caaaaatcca
      901 gtcgggtttg cttccacccc gtaatatcca ttggtatgtt tcaatcatct ttctcgtgac
      961 ctcagtccat gaaaattcta tccgaatcca ttcacgaccg cgaatgccca tagcttttaa
     1021 tatatcgcgc ggtttcgaca gggcaacctc caagcaagct actagcggat caacaccgat
     1081 atcaatccac caccctgccc caagagattc aaggttgcgc catggggtac ctttggttac
     1141 aatcactgga atgcctacag agagggcctc agcaaccgat atcccgaaat tttctgacgg
     1201 acttggcaga acaaataatt ctgcatttcg ataagcctca attttttttt caccgtagag
     1261 tgcaccaaca aattcaattc tttttagacg taatttttcc tttaatgcct tcatcttaga
     1321 taagtaaccg ccactgtcgg gtcctacaat cttcagttgc cactctggaa atcctcgtga
     1381 aacaacctgc catgcacgaa gtaaattatc aactcctttt tccaaatgaa tcctaccaag
     1441 aaacagaagt gttcgtgtat ttccaagtgt agtttcttta tgctgtggca tatcaattcc
     1501 gttaggtata atggctatcg gctgtgagaa cctaagccga cgaatatcct caaattctga
     1561 catcgctgta gcatgaaagc agttcgcagc atttacggct ggacgttgaa tgaggggcca
     1621 gaacacatgt tttattttgg aaccactagc catggcatat tcagcaaatg taccgcgtgg
     1681 cgagattata taaggaatat tattttttct agctatattc ccaggataaa tatttggcat
     1741 catccacagg ctatgactat gaaataggtc aacttttttc atctcagcct gttttgttaa
     1801 ccatagccgc attgatgagg atagcccagg ccgtctacga ccaagtgtaa ggggaaactc
     1861 tttcaaaaat cctggtgtct ccgtcattac atcccaatct aacgtcgcta atattacctc
     1921 ttgtttgttc tcaattagcg attcacagag gcggcgaatg gaataagaag gtccactggc
     1981 ctgttctgct atggaatgga taagatgtac gactttcata tacagcttct cgactgattg
     2041 tgcatatgaa tcagcttatt ttaccttatc tttggtgctg acttaattca gtgttgcatt
     2101 acctcttcat tactgtggaa gatagcatta acgtttttct taattatcag gttgcaacgg
     2161 tttttacata tatttcaatc atattcatta ggttgtcggc tttatggctt tatgcctttc
     2221 aaggcgagag taagttgccc accccataat tagccagatg tatagcccta aaccaccaac
     2281 tgaaaaaaat atcatttcgc cgatattgag aaataggcat gtaagaaaaa ccaagcttag
     2341 aatcaaatta ccatttgaaa ttaaaagctt catcaaagta aatagaaaca gagtaaaaaa
     2401 tgctgtacca ataattccag tttcctcgag aagagcaact ggcaacatcg gcttttccgt
     2461 tggtgccgca agtggcaaac ctgttaatgg ctcaacaact ggatcaaagg gaataaaaat
     2521 gctgtcactc acgccaaatc caataccaaa ccacttatgc tgtatgaaat tatccatcga
     2581 ttcaacaatt agaaaacccc ttgacttctt aaatgactcg gagatgccca tatctggcaa
     2641 atccttaaag atgaaagacc taactgcttc cagtacagta gggggccgaa aaagagcaat
     2701 tgcaaggtat gatataatta aaacaagaaa aataggacta aatatggatc ttcgcagcag
     2761 ttttctccat tcgttacgct taataagtcc aatcaaaatg acaaccgtaa aacccatgat
     2821 aactgcgaca agtgcggtcc tccctctagt cagaaacaat gaagcccaag caagagtcaa
     2881 aatggaatat aaccaataat gtcctttcaa aggcaaaaat aataatcttc ccataagcca
     2941 agccaccatt ggtaccaaaa atattgcaaa tgtttgcgga tgattgagaa gcccttggaa
     3001 acccattcca tctaaaaaga aaccaatcct tggaaaaaaa aatgttggag cagaaagtac
     3061 cgttacagca atccaaatac ctagaaacca ggatgtccaa tcaactgact tattggctgt
     3121 cgccttgaat ccaattaaaa ctgcgagtgc taggtatgcg aaggatgcaa tcttaaatgt
     3181 tgacaccact ctgtaataac taaaaaatat agattctaaa agaatcacag cggagaatag
     3241 taacagccat agcagtacca attgaatctt ccaggtcacc cgaagattat cgataaagat
     3301 tcttgctcca gctagggcga tgacgaccca tcccagcaag cttatagggc cttcattagg
     3361 atagatcgca ggattgagaa ttttgatcag aaccaacaga gagagtgctt gaatagcttg
     3421 cgctgttccc ataagcgacc aaatgcaaag aagaatcagg acgaaacaag atgcatacgg
     3481 atgaagagct agcactgtaa gtaaaaaaac tatgagtata gccactctta gagtttggct
     3541 aagtgtgaat agctcagatt tgtctatcac tttggccaat attgtttacc tattcccaac
     3601 tctgatcaaa ccccgtcgat gagccactaa gtaggcaaac acagcttgaa ttaatgacgc
     3661 cagtccataa cttagcgctg cacctattgc accagaattt ttttgagacc aagtgaatac
     3721 aataaagaga acaaaccaga ctatgattat aaataaccat gcccattgtc cattatcagc
     3781 aataattccg ttacctaata caccagttgg agcctgtggt agagctgcaa acatgaacca
     3841 cataattaac cagtgatatg atgtataatg agatccatat aagcacatta gccaaggact
     3901 gccaacaaca gcaatacagc aaattacaaa tgctgtaatg ctggctagca tcattccttg
     3961 atgaagaatg acagatgttt gtaagctcgc atttaggctt tcaattagcc ttaccctaac
     4021 aagctgagga aaaatcactc gagtaatcat gcctggcaaa taaagaacaa tcgccaacca
     4081 ttgcaagcct attacgtaaa gtgagaattg ccagtggcct gacggtccac tcaataaaat
     4141 ccttccaaca aaccatgttg ccgaactagt cattaagcta atagttgcca ttggcgcagc
     4201 gatacggaat atccttctta tttgaaatcc tccaatccat aaaccggtaa gtaacggacg
     4261 tagacctatt acttttgtaa caagccatga gttgatcgct gtctgaacga gggatgcaaa
     4321 gatcaaaact gacattgcag attgtggtga attaagtctt cctgctacta acgcaccaac
     4381 cacaataatt acagaagaag cggctgatgc ccaagttgcc acaacatact tctccaagcc
     4441 aagaataccc cctcctggta tcaccccaag agataaaaca aacactccaa ccgccaacaa
     4501 ccaacttggt atattaagac cagattcgaa ccatgaggaa gggacaagca gaacaactag
     4561 ggccatcaaa agacccgaca aaaccgaaag cgcccagagc gtacccaaag ggggcatggc
     4621 gtcaccagat tgctgatcgc tctcagcaaa gaactttgac gctgttacac ccaaacccat
     4681 tgcagcgtaa gccgcaagca ttgacaccgt catctgaaaa taaccatatg ctgcaaaacc
     4741 ttccgttgcc agcatacggg ataaaattat tgataccaga acaaggctgc cacgctctaa
     4801 taaatgggct gctgcgctcc atcctgcatt tgttagcaat gtcttcatga ccagggatgt
     4861 tcagtagccg ttcgggatgc aacatagcag ttacggttat tttcatgacc gtatgtctcg
     4921 gtaaaagcat ttaacccttt cagaccagag cttgaaatgg ccttggcata tttacattct
     4981 gttgttgtga agcgaccgtc tttttcgata atcagatcga cttcgtcacc ccttgctgtt
     5041 ctccagaacc atatggggca acgtctgaat atgaaggtta ccaccaccgc aggaaactga
     5101 gcaaataagg tctttagcat tttttcgtat attcttgtaa tcttcattgc aaatattttg
     5161 ctatctaatg caaaattgtc attgttataa tatcgagcga gtggaagcaa catcttgaca
     5221 ccttcactta atctcgtcag gcagttaacc ttattagtga tattcttcca atgttttcaa
     5281 acaagtaagc ctgccggcca cgcagctatc tggcaagatt accggttggt acactattga
     5341 accagcaagc tctccaggtg ggcgagtacc tttttgataa tttgactgtc aagctcattt
     5401 ccatagagcc taagaaagag catctttctt ttctcggtaa ctgaaagatt gggccgcgat
     5461 gccagcatta ttgtttttgc agtatcaaac atggcaaagc ccatgcgcat tctctcctga
     5521 ttgtttttct ttctaaattt attacaaatg cttgctgcaa tcttttctgt tgtaccattc
     5581 atgaccggca ttcttccaaa agagaatcaa ctgtgagtcg ttttgaccaa tctctcaaat
     5641 aattgaaatc tatatcatct gttatcatca ggatgttttt tacatctctc ttctgaatct
     5701 ctgaattacc ctcatcttgc ccacaggagc ttgttaggaa caagatcttc cggtgatatc
     5761 aggcagatct gaaaatcacc cacgttaaca agcctgcgtc tttcaaaagc attaatatcg
     5821 tattttgtgt cttttcttaa aataaaatca acctttactg tataatcatt gagtataaca
     5881 ttaaacatcc ctttcccaga gacctcattt aaaaccattt cggaatcaat atagaattct
     5941 tctttgaaag catcgataaa tctccttatt tgtgtctctt tgatagaaat tattatatca
     6001 atatgacggg tcatcctcgg ttgaccgtaa aaattcatcg ccacagaacc agacaacata
     6061 tactcgatat ccaggtcgca gagcttttct accacttttc ttaatacttc aagttgttcc
     6121 attaggattc ttggtaatac atcaggtacc aatcaacaaa tttctttatg ccctcgttaa
     6181 ttggcgtggc cggtttaaaa cccacatcat gtatgagatc atctacatct gcgtacgtgg
     6241 caggtacatc cccagcttga aggggtaaaa gatttttctt cgctttcttg ccgagagttt
     6301 tctctatggt tttaataaat gtcatgagct ctacaggatt gttgttgccg atattgtata
     6361 ccctgtaagg ggcgtagctt gttccaggat ccgggttatc accactccac gatttgttcg
     6421 gccttgggat cttcttcatc accctcatga caccctcgat tatgtcatca atataagtaa
     6481 agtcacgctg catattgcca cggttgaaca catcaatggt cttcccttca aggattgttc
     6541 gcgtgaagag aaaaagagcc atgtctggcc tgccccaagg gccgtaaaca gtgaaaaacc
     6601 tcagccctgt tgttgggata ttgtaaaggc tcgaataggt gtgggccatg agttcgttcg
     6661 ctttttttgt tgctgcatag agcgaaaccg gatgatcgat gttgtgatga atggaaaagg
     6721 gcatctttgt atttgcgcca tagacagagc tcgacgatgc atacacgaga tgtttgatct
     6781 tgtgatgcct gcagccttcg aggatattca taaaaccgac aatattgcta tcgatgtatg
     6841 cataggggtt ggtaatcgag tatcggacac ctgcctgggc agcaaggtga acaacagtgt
     6901 cgaaagtacc cgtgacacgt gatgggtggc acgtgatggg tggctcttta attttgagtc
     6961 ggggtacccg ttcgtgggtc gcctgagttt tgagtaaacc gttgaacaaa gactccatag
     7021 cacggcggtt agcgatatta atcttgtgaa aggtaaagtt gtcatatttt ttcaaaatgg
     7081 cgaggcgagc cttcttaagg tttacatcat agtagtcatt caggttgtcg ataccaacaa
     7141 cgcttctgcc ttcttcgagc agccgcttag agagatgaaa cccaatgaaa ccagccgccc
     7201 ccgttacaag tatccttcct ttcatgacat tctctccagt aatttattca gacctttcac
     7261 gccgaataga aaaatttgct cttccccttc actatagccc ttgtatcaac tatggggatt
     7321 ccaaaagatt tgaaatcgat ctctctatac tcgtcatggg ccgtcgcgat gaggatacaa
     7381 tcgtaattgc catttatttt cgcagatttc cttcctgcat accgggcata ctcccttgat
     7441 tttcgtataa ccggaatata tgggtcgttg taactcacat gggcgccgag tttctcaagt
     7501 ttttctataa gccggtaaga tggggattcc cggtcatcat ctacattcgc cttataggca
     7561 agtccaagaa tcaatatctt tgcccccttc aaaggtttac caacgtcatt taaggctttc
     7621 atcacctttg agaccacgta atccggcatg ctcgtgttta tctccccggc aagttcaatg
     7681 aatttcgtcg aaaattcata ttcatgggcc ttccacgaaa ggtagaacgg atccaatggg
     7741 atgcagtggc cgccaaggcc cggacctgga tagaatggct ggaaaccgaa tggttttgtc
     7801 tttgccgcct cgatcacttc ccatacattg atgcccatac ggtcgaaaag tatcttgagt
     7861 tcattcacaa gggcaatgtt tacggaacgg tagatgtttt cgagcagttt tgtggcttct
     7921 gccacctttg ttgatgacac cggaactgtt ttgatcacga ttgtgtcgta gagagctttt
     7981 gcagccatca agcaattacg ggtataacca cctaccacct tgggcacagt tctcagactg
     8041 aagtctttat ttgcagggtc ttctcgctca ggtgaaaatg ccagatagaa gtcttttcct
     8101 gctttaaacc cggtcttttc aaggatagcc ctcatatcct cgtccgttgt tcccggatag
     8161 gtagtcgatt ccaaaataat caactggcct ttttttagag actgtgcaat ggttttcgtg
     8221 gtgccgaaaa cataggtcat gtcaggctct cggttcttat tcagaggtgt tggaacacag
     8281 ataaggatgc aatccatgtt tttcaacata gagaaatcag tagttgcgtt aaagaccggc
     8341 tgcaaaccgc tggtcacgag ccgctcagat tttgaataag gagcgacagc gctgctttca
     8401 gaattagtct ttagccctct gcctcctgct ctcttccctt tgcttgtaag cggcgctata
     8461 tcgatatgtt tgatataggt ttttcctgcc ttcagaaaat cgactttatc ctggtcaata
     8521 tcaaaaccgg taaccgaaaa accctgtttt acaaactcaa taatcagagg cagccctaca
     8581 tatccaagac caatgacacc taccctggcg cttctgtttt caatctttct tacaagatcc
     8641 atcaatcctc ctcaatcata ttccgaattg gagaataaat atgcaaaaat aaggccaggc
     8701 taccgcctct ggccaagatc agggacctaa atatggttat ttttttatac aaatatataa
     8761 aactgctctg ctgcttacca gattttgagc tgctttcctg tccttaccgt agcctttatg
     8821 gtgtacgttt atatcactta agcattggtt tttcaaggaa aacgctgggg ttcaaaccat
     8881 tcgaaaaatg ttgacacaac accttcttcc tgttagcctt ttgaaaaatt caaggtaagg
     8941 aggctttatg aggtcagacg gcagggttgc aagaataaca gaagtggttg caggttcacc
     9001 aaaaaacttt gacgatgcgg tgcttgttgg cttcaaacgg gctgcccgaa ccttgcgggg
     9061 tattacgggt ttgagagtta aggatatgcg atgcagggtt gaagagggaa agattatcga
     9121 ataccgggta acccttgaag taatttttat cctcgagagc tgataaacag tccggcatta
     9181 tccggacatc cccttaaatg ccagaggagg agcttcatat agtatgtata ggtccttctt
     9241 gttctcctgt gcagtagaac tgggaacagt acagtaagga caagagggac cacaaagctt
     9301 ttcatcgctc tgactccctg aaacatacga tagtagaaaa ttgtatgatg ttttcgaatg
     9361 aatttataca gggaaatatt gtactggatt ctgcccttta tccactgttt gcgtacagtc
     9421 cttccctgaa ggtgaattac cctgacatga gggagaaaaa caacacgcgc cccaatttcc
     9481 cgggccctga tacacaggtc agtctcttcg agaaagaaaa aaaatcgttc atcaagccca
     9541 ccaaggtcag tgaggagttt tttctgtaga ataattgccg cgccgataag ggagggaaca
     9601 tcgaagcatt gctgcgcggg cgcaggacgg tgggcgagag gcgatgggat atgtgatgcc
     9661 gaatcttcct gtaaagactt tgtggaatgc ctggccggaa aaagaatctc cataagactg
     9721 cgaggtacaa tctcgaaaag aagtgagggg atatttgcat aagaacgctg atgcctcatg
     9781 tcctcatgga caagctgagg gccacaaatt cctacatcac gattttcttc catcaactgc
     9841 accatctttg caacttcccc ttctatgagc aaggtatcag agttcaaaag gaagatataa
     9901 ctgccctcag cccgggcaat ccccacattc gctgctgcgg caaacccaag gttatattcg
     9961 ttcttaacca gaaaaaccca tggaaattca ctgtctacca tggtatttga accgtcagtt
    10021 gaataattgt caacgatgat gacttctttt agaaaaggcc tgaggagttt gtctgcttca
    10081 atcgatttta ggaggtttcg aagaagctct tttgtgttcc tggtgatgat gacgattgat
    10141 aagttctgtc cggcgttcga ggttcggcat tcggtgacat tcatactaat agcggtgggg
    10201 ttattgcaag gcacatggta cagtgtcaat ccatgttgct gaaattgttt ctttcatatt
    10261 ctacaggtca tgaaccactt ttccgtcagt gggacaggtt ttttcctttt cactttttac
    10321 tatcttcccg cccttcccgc cttttcttct catttcttac ttttttcaac tctttcaggc
    10381 tttttccttc atcgcttgtt ggacatgcgt agtagcacat accgcacctg atacaaagcc
    10441 cctcgtcaat acatattttc tttttttctt catcaaaata cgaaccaggg cagtcgaacg
    10501 tcttgatgca gattttacat cctgtacatt ccgcaacgag acgggccatg tcacgggtgt
    10561 cttcctccct gatttttgaa aagagaaggc attctctgcg ggctatgata acagcaggac
    10621 ctgcggattc tgtttccagg tattcgtatg cctcttttat tgtcctgagc aagaggggga
    10681 tatcgtacgg gtcgactatc cgcaggaatt ctatgccaaa gcctttgatg atgttttcta
    10741 ttttaatgga atacgttgac gtgccatctg cagtgatacc agattgtggt gtcggctgca
    10801 ggcctgtcat ggcagtcgtt gaattgtcca taataacaag aacaaatcgt ctcttcttca
    10861 aaacactatc atacaatggc gtaagacagg catgaaagaa ggttgagtct ccaactgaag
    10921 caaggatcgg ttttagtgta ccatcctggg caaaggtgtc atagaaacca gaagcgaagg
    10981 tcactccacc tcccatatcc aggcacgtgt caacagcacc catggagata ccaagggtat
    11041 agcagccgat atcgccggga aagacagcat caggaaatgc cgatttcata gcatagaacg
    11101 aggccctgtg agaacaacca gcacaaagct tgggaggtct tggcgctatc tgaatctctt
    11161 tcagtgcatc ttcaatagat gtgtctgcat tgaattcagg aacacttatc ccatgatcgt
    11221 ttgcaaagcg gcaaataata tctctcacat tatcataggt cagctctcct gcactgggaa
    11281 catgaccgct ccttctcccc agaaccttga atccatggtc cagaagcgcc tccagcgcta
    11341 catctgtctc ttcgaggact aaagtttttt cttgctgatt aacaaacccg attacttcgg
    11401 tgccaagggg aaaaaccctt gacaccttat ataagcctat tttatcccca agattcagct
    11461 catcgagaat atccattgtt attgagcatg tcattccgga cgttacaatg ccaatcctgc
    11521 cttttccctt gagcttaagg ccttcatcca gtttaacctt tcttgatcca ggaacaaaaa
    11581 gctctatcga ttcccgtgca tgactaactc tgtgggtgga tctgacaatg acgggaactt
    11641 tatgctcaca ggaatacttc atggcataat aggcgatgtc gccggcctct ccaggagaag
    11701 ctggatcaaa aacagggata ccaaaatgag cagcgaggag ccgcgtgtcc tgctctgtct
    11761 gcgaagattg aggccctggg tcgtcacagg agacaattac cagaccacct tttaaaggtt
    11821 gcgcataacc gtggagaaga ggaggaaacg caacattcaa gccgacctgt ttcatcatac
    11881 atgctgcctt tcttccagcc aatgctccgc caaaagccac ttcaagcgca caccgttcat
    11941 tggttgacca ttcggcatgg atggctaact tctccctgtt tttgaattca atgatcccgg
    12001 gtagaatttc agagcttggt gttccgggat aggctgcagc gagctcaaga cctgcttcca
    12061 caaggcccct tgcgatagca aagttgccga tcatcacttc tctcatattt cctgcctcat
    12121 tcttcatgaa ttattggttt ttgctatcgg cactgagctg aaagctttga gctgactcat
    12181 acccggcctg aaatacctct aaattcttct ccacgagcct cgccttcacc ttctgcctga
    12241 tggtctcttt tatgttgtca caggaaaaag gggttatgtc aaaggcagaa aagaaaccga
    12301 gcattgccat attcgatgac tgtatcatgc ctttctgcat tgctattgca tcagcatcca
    12361 gaaggaaata ctgtaccctg cgttttttca gtaaacgttc aatgttggcg ctcaacaatt
    12421 gtctggatgt gttaacaagt gttactcccc ctgtctttaa aaagggcagg tttcgataga
    12481 attcactctc ctcaaatgaa aggaggatat tggcactctc tcttcccacg agaggacttg
    12541 caaaattacc gacttttaca agagacacga cagagccgcc tctctggctc ataccatatt
    12601 cgtctgaggc cattgctttt aaacctgctt ttatggcagt ctcgataata atcgttgagg
    12661 caagcaaaac cccccttccc ccaattccag cacagacgat ctgtaagttc atagcaaata
    12721 atccctttcg aacatcgtat ttcctatgtt gtatgtcgca aaaattattg tttcccattc
    12781 agcttgtaag cctgccagct tgacagcccc gggcttgcta accaggcctt tttcctttcc
    12841 tctcacgtat cacttcttac tttctcgccc gtctttcccc atctcctatg gcctattgcc
    12901 tgttttaaat tatcccccgg caaaacagca ctgtcatatc ttcggtatct ttgaatcgtt
    12961 caatttccga aaaattgtct atgcgaaata cattgttaaa gccaaaaccg gcaagaatct
    13021 tctttatctc ttcctctttt ttgtccccaa gaattaggat gatatatgca gacccattgt
    13081 aaagggtatt cacaaatgcg ggcataccgg aatgcaaaaa gaaatcctca aaagtaatgg
    13141 caagaatctt tttttctggt tcggtctttt tgataccgtg agccatgttt attgatgacg
    13201 cgggaccgag tgtgtccctt actacatcaa aaccatacat ggtctcctga ggtttttttc
    13261 tttccggtat gtcccccacc ttcgcagata caattttcga tctgtcctgg atctggagtt
    13321 ctatggccga atatggccct tctgctatga agacttcctc cattccagca ataaagtcat
    13381 tcaccagttt tacaggtaag gggtgaaccg tggatataaa aagcaggctt gaatcctcgt
    13441 caaagaagtc caggttcgtg tgtccattgg tgataacacc ggttttgctt ttaatgcgtt
    13501 tcctattgcc ctcataggtc tcaaactcgt agcggatctt ctcaatcttt tcatttaacg
    13561 ccctgtgaag ttcataacgg aacttcggtg ttgcagccca tcggcccgga ttcttgttga
    13621 ataccgagtt attaattttg aattctgaat tttgaatgga agatgagctc tttccctcgg
    13681 tttgcgccgc aacatcagga gctacctgta ctatcacagg aatctcgtat ttctcggaaa
    13741 tctcataccc aaaacctatt gctttcttga attcatcctg gctttcagta acaacaagag
    13801 gcagttttga gaaaagacca aggggcgtaa tctcctcctc ggtctctttc acacatacaa
    13861 tgacaaagcc tccgataaca cctgtataag cagagctcat caagggatcc agggcttcgt
    13921 aaagaccttc tgtggaaaaa aggcatgctg ttctctttga agaatatgct ccggtaaggg
    13981 caagctcaaa ggcaatcttc tcgttaagac ttgtctcacc gcgaagggat gcatactcgc
    14041 tctcaagcat cttcaccaca ccacctgctg ccctgtttgc cgtgtaaagg aggcgtgtac
    14101 cgttttctgc taaaaccctg caaacctgat cgtacatggc ttacctcgat ttcaagtttc
    14161 gagttacaag ctacagattc aaagcacttt cattttaaat catatatata cagatattta
    14221 acatctttta agttcacaaa caagttgttc tatatcctca tcaggcatta tataatcatg
    14281 caactttcct gacagatacg catcatatga tgcgagatcg atgacaccgt ggccgctcca
    14341 gttcataagg atcacctttt cttttccttc ttccttggct ttcactgcct cgtcgatgac
    14401 acaggcaatt gcatgatctg tctctggtgc agggataaat ccttcggtgc gggcaaagtg
    14461 aatacctgcc tgaaaagtct caagctgagc gtaggccttc gcttctataa ggccatcctg
    14521 gagaagtctg ctcaccaggg gtgccatgcc atggtaccgt aagcctcccg cgtgaatagg
    14581 agccggcaca aattggtgtc cgatagaatt catgggcaac agtggtgttg tt
//
LOCUS       DAOOMC010000075        14450 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdus sp. isolate AMR_MDS_2685
            NODE_4499_length_14450_cov_6.719347, whole genome shotgun sequence.
ACCESSION   DAOOMC010000075 DAOOMC010000000
VERSION     DAOOMC010000075.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864760
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdus sp. (wastewater metagenome)
  ORGANISM  Syntrophorhabdus sp.
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae; Syntrophorhabdus.
REFERENCE   1  (bases 1 to 14450)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14450)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,729
            Genes (coding)                    :: 2,708
            CDSs (with protein)               :: 2,708
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 7 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14450
                     /organism="Syntrophorhabdus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4499_length_14450_cov_6.719347"
                     /isolate="AMR_MDS_2685"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2699756"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..602)
                     /locus_tag="PK125_08670"
     CDS             complement(<1..602)
                     /locus_tag="PK125_08670"
                     /inference="COORDINATES: protein motif:HMM:NF013714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HPB38221.1"
                     /translation="MDQTIIDEIIKIVGKENALTSHEERKCYSFDARTDGTIPDLVVF
                     PTSAQEVSQILILANKHRFPVIPRGQGSGLTGGSIPINGGVVLTFTRMNKILEIDAKN
                     LVAVVEPGVITFVFQEEVAKYGLFYPPDPASYKYSSIGGNVAECSGGPNSLKYGVTRD
                     YVLGLEVVKGTGEIINTGVKTMRGVVGYDLTRLFVGSEGTL"
     gene            complement(712..1779)
                     /locus_tag="PK125_08675"
     CDS             complement(712..1779)
                     /locus_tag="PK125_08675"
                     /inference="COORDINATES: protein motif:HMM:NF015948.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HPB38222.1"
                     /translation="MYKVLIHHATYETVSEAIKKAFEVFPFDLCGKKVLIKPNVLRAS
                     QPEEGITTHPMILRAVVDTVEMMKPISIIVGDNSGVFSYGANEDAFIKTGLMEAAKGY
                     YQNIGTDSIKVDFNPDFLSTISISRVALESDFIISIPKFKTHGLTVLSGGIKNSYGFL
                     PGAQKARLHKAAGNPERFNNLIVEVFRIRVPDFFIVDGIVAMEGNGPASSDLRETNLV
                     LASDNAVALDGVMATMMGCDPGRLRFLQRARELNLGDYDLNHLEIVGDITIIPDFKLP
                     PLGGEAVHSNRAMENLLNSRVLLKPRVDEGSCTGCGTCVGQCPVSALKMSDRFPQVDT
                     DQCITCFCCQEICPSKSIILQ"
     gene            complement(1891..3561)
                     /gene="ilvD"
                     /locus_tag="PK125_08680"
     CDS             complement(1891..3561)
                     /gene="ilvD"
                     /locus_tag="PK125_08680"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF002068.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HPB38223.1"
                     /translation="MRSDKMKKGIEKAPHRSLFSAMGYLPDELERPIIGVANSANELI
                     PGHIHLHRVSQAVKDGIRLAGGTPMEFSTIGICDGIAMNHEGMKYSLGSRELVCDSVE
                     VMAKAYPFDGLVLIPNCDKIIPGMMMAALRLNIPCILISGGPMLAGRFKGRPVDLISV
                     FEGVGKVAGGLMSEEELGLLEQCACPGQGSCSGMFTANSMNCLSEALGIGLPGNGTIP
                     AVHAGRIRLAKTAGQKVLELVEKNLKPRDIMTLDAFKNAIAVDMAFGGSSNTALHLPA
                     IAHEGGISLPLSLFDEISEKVPHICNMSPAGPHHLEDLFEAGGVYGIMKELSKKNLIN
                     KKCLNVTGEKLGDLLKTAAVTNPEVIHPLDAPYHEKGGLAVLSGSLAPQGAIVKRIGV
                     QEKAWHFVGKARVFNSEEEAGAAIMAGKIKEGDAVIIRYEGPKGGPGMREMLTPTSIL
                     AGRGLDTQCALITDGRFSGGTRGLCIGHVSPEAAEGGPVAFVKNGDRIEIDLVNKRID
                     IHVNKAEMEKRKRAWKKPQPKIKEGYMARYAKMVTSAATGAVFKDPDN"
     gene            3797..5566
                     /locus_tag="PK125_08685"
     CDS             3797..5566
                     /locus_tag="PK125_08685"
                     /inference="COORDINATES: protein motif:HMM:TIGR03904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HPB38224.1"
                     /translation="MSGFLPTTKEEVAKKGWDSLDVIIITGDAYVDHPSYGAALIGRV
                     LENAGYRVGIIAQPDWRSKKDFIRLGKPRLFFGITAGNMDSMVANYTANKRLRKKDAY
                     SPGGRPGLRPDRATIVYANRVREAFENIHIVIGGIEASLRRLAHYDWWDNTVRRSILL
                     DARADILVYGMGENQVKEIAHRVDKGIDLTGIPGTAIVRKHLNPFVNMEIIHPYEVVK
                     SWTDRFNEAYKAIYYNQDPVNGRIVAQAHGNRFVIQFPPARPLETQELDAIYELPFEK
                     NPHPSYQKKGIIPGFETTKFSIVSHRGCCGECYFCSLFMHQGRIVQSRSGSSILNEAR
                     SLCQRHDFKGTISDVGGPTVNLFMADCTLWHKWGACKNRHCLIPEKCRNLHLGYEEGV
                     QLYKEILKIPKVRHVFLESGFRHDLLANNKASRYLEHLCRHHVSGRLKVAPEHNIPHV
                     LRLMNKPDFNIYEAFVRKFREMKKKTGKNQYLVNYFISAHPGCTLQDTLELAIYLAKR
                     RVHPEQVQDFIPLPMTVSGCMFYTEKHPFTGEDVHVTKTLHERKIHRALIQYKNPSSK
                     KYIHEALKKLGKEDLSRYFLQKS"
     gene            5617..8214
                     /locus_tag="PK125_08690"
     CDS             5617..8214
                     /locus_tag="PK125_08690"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HPB38225.1"
                     /translation="MKSTENINQLITELDTLRKRVYELEQYEAERTICENTSKKTEEA
                     FLQLFNSTEEIAFLQELDGTIVIANENSKHFYKILEGNIVGKSIFDLVPEDRVENAKE
                     KVKAVVDTKKTVRFEGTLGQSVFENSLYPVFDQEGNVRRIAVYVRDITIRKRLESAVH
                     QAEEKYRTIYENAMEGIFQTDLEGRFISANPALASIHGYDSPEELIKTVTDIPHQLYV
                     NPTDFTNLSKLLHIQGEAQGVETQMYRKDHSRHWIVINVRTVKDEQGKVQYYEGTMLD
                     VTNRKLAEEQLKESEERYRIAIEHSNDGVAIVRGDIHEFVNRRFLEMFGYDSLDDLVG
                     KKVFVIVHPDDREKVVEINRRRQTGQPVPSRYEFKGITRDGRVIYVDVSAATISYREN
                     LVYLTYLRDITERKKAEEALRNERNRFQALSDSAPFGIMVIDEQGNFTYLNPKFTELF
                     GYDLNDAPSGRIWYKKAFPDIKERVAAMSGWVRNVKNTTPGQRIAKIFTVTCKDGTKK
                     TVNFIPVRLATGEYLVSLDDITERIQAQNALLQSHKELENLNRAKTKAVNHISHELKT
                     PLAVIQGNIRILKRKIQSLSGDGSLEGIIAVLERNLERLFGISKETDEIFRVTQEVEA
                     NVLLDDLERLWERIVDLSDLPEDMQEHLNALKEWVSQYRAGGILSAQSIDLYPFIMQT
                     LEKAKHSAQARKIDYTLEGENDLYISMDPVILREVAEGLIKNAIENTPDGGKIDISVE
                     QKGDRIWLHVKDYGIGIREENQQYLFDGLFHAHETDLYSSRKPYDFGAGGKGLELLRM
                     KVYGQRLGFDITMKSKRCIYIPTDQDVCPGDVTQCTHITSPEECYASGGTTFSVSFPA
                     EKKHSQTEV"
     gene            8278..9645
                     /gene="rlmD"
                     /locus_tag="PK125_08695"
     CDS             8278..9645
                     /gene="rlmD"
                     /locus_tag="PK125_08695"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: protein motif:HMM:TIGR00479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HPB38226.1"
                     /translation="MTPSEFSVEIIDIALPNNYGVAKKDGRVIFVPGAVIDDKVTVRL
                     NRESKRFAYGEISHVDVPSSFRVTPECPHFGSCGGCIMQNLRYDKQLLIKQKYLLDNL
                     NRVGGLDPQSLEILPVTPSPDLYFYRNKLELAFGNKNDEVILGLRERLSPFKPFSANV
                     IPLNKCPVFSTVAEKVIPILTQFAHAEGFTAFNPLTRKGILKHLILRESKSTGEIMVI
                     LETKTEALQGIKNVIHEIGQRVPGVVSFYHATSRRTDDIIHFDRINRLFGARSIVEHV
                     SHLNLNIYPQSFFQPNSKSAALLYGEIANQLNLKGSEVILGLYSGTGSIEIFLSDKAL
                     QVIGIDSEQANISTANKNCKANQIENCKFYQSRVEDILKQCKLPKADILIIDPPRTGL
                     SGQALHVVKHVAIPKIAYISCSPPTLARDLRELCGYGYSIHRIIPFDFLPHTGHLEIL
                     TILVH"
     gene            9777..11786
                     /gene="ligA"
                     /locus_tag="PK125_08700"
     CDS             9777..11786
                     /gene="ligA"
                     /locus_tag="PK125_08700"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF005932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HPB38227.1"
                     /translation="MEKKEIKERIEKLKGQLEYHNYRYYVLDDPVVSDTEYDRLMTEL
                     VALEEDHPEFLSPNSPTQRVGAKPLAEFETVTHTIPMLSLQNAMELDEVAEFDRRIKK
                     LLRINDVDYVMEVKIDGLAVELVYLNGEFTTGSTRGDGFTGEDITQNLRTIKSIPMRL
                     LRDHDIPIPDRLEVRGEVYMGKNEFKELNKERELQGDPLFANPRNAGAGSVRQLDPRI
                     TAGRKLNIFCYAPGEILGVTLTTHYHFLDYLKKWGFRVNPLTKLCRNLDELISHYNYI
                     QNMRDRIPYEIDGTVIKVNRFDYQVLLGNVSRSPRWAIAFKFQAHEETTIIEDIIVGV
                     GRTGALTPVAVLKPVMVSGVEVKRATLHNEDEIVRKDILIGDTVIVSRAGDVIPEVVK
                     VIKEKRTGQERPFTMPEECPVCGEKVVRQPGEAIRRCVNINCPAQIKGSIEHFASKRA
                     MDIDGLGEKLVEQLVDKKAINDVSDLYYLKKDDLLQLERMADKSAQNLLDAISTSKKP
                     SFQRFIYALGIRNVGEHISGLLADKFNSIEDLYTLDEDTLMAIPEIGPEVANSITSFF
                     QDDKNRKTISRMLDAGVVIEYKKEGRKPLLGLTFVFTGALKNMGREEARKKVESLGGK
                     TVSSVSKKIDYVVLGEEAGSKVDKARSLDLKIISEEEFLSLIQKS"
     gene            11917..12609
                     /locus_tag="PK125_08705"
     CDS             11917..12609
                     /locus_tag="PK125_08705"
                     /inference="COORDINATES: protein motif:HMM:NF013232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MTAP family purine nucleoside phosphorylase"
                     /protein_id="HPB38228.1"
                     /translation="MIGFIGGTSLTNSTIFKGWHQNSIETPYGTVILKKRQSSVFLQR
                     HGSPYLPPHRINHHANIWAMKHIGVEEIIAINSVGSLKHAIKPGTFLIPDDFISIWYI
                     PTFFNDKMQFTVPRMDTESADRLYRQCEPLKIPVKKGGIYIQTQGPRLETKAEIGMLK
                     RFGDIVGMTMASEVTLCLECGIPYASICSVDNYCNGITKIPLTMDEIQKNVRNNLQNI
                     ERLIDSIHQGRL"
     gene            12609..13865
                     /locus_tag="PK125_08710"
     CDS             12609..13865
                     /locus_tag="PK125_08710"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HPB38229.1"
                     /translation="MTILIKSVMVNGKIQDIFLEDGVVCEISDECIKIADRVIYGQNK
                     AALPSFINGHTHAAMALFRGYADDMPLKSWLEEKIWPLEKKLTEEDVYWGTKLACLEM
                     IKNGITVFNDMYWQWKATAMAVKEMGIRGFLSAVFIDMFDQNKSAEEIDLNVRLFEVS
                     KQFQPAVTFTFGPHALYTVSRESLEWIRDFSHAENILIHMHLSETEDEIHFVKEKYGL
                     SPTEFLHSIGILSDRFIGCHGCFLDKNDCSILKRNGAKLVHVPVSNLKLAVGRIFPYA
                     LAKEYGIPFCFGTDGCASNNDLDLIETVKFAALLAKFSTHNPTMMPAKEAFNHATKTA
                     AAIFGMGEWEIKVGASPDIILVNLNCPEMTPDFDLYSNLVYASKGSIVDTVICMGKVI
                     MENRTVPGEEEILRTTSKIAKSLANR"
     gene            13862..14350
                     /locus_tag="PK125_08715"
     CDS             13862..14350
                     /locus_tag="PK125_08715"
                     /inference="COORDINATES: protein motif:HMM:NF014037.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mut7-C RNAse domain-containing protein"
                     /protein_id="HPB38230.1"
                     /translation="MKFICDAMLGKLAKYLRILGFDAEYTKDSKILEHYKGKTDAPYF
                     LTRTIKKGGYERTIVILSDKAREQLREIRELIHPFIDSAKVMNRCIECNIDLMGVQKK
                     HIEQKVPEFVYHQYEQFKECPRCRKVYWEGTHTAGMTELIREIMEHADMKAEKTVQAE
                     KM"
BASE COUNT         4345 a         3208 c         3088 g         3809 t
ORIGIN      
        1 agagtgccct cactaccaac aaaaagacgt gtgaggtcat acccgacaac acccctcatc
       61 gtcttgacac ctgtgttgat gatctcacct gtccccttca caacctcaag accgaggaca
      121 taatctctcg ttacgccata ttttaatgaa tttggacccc ctgaacattc agcaacattt
      181 cctccaatac ttgaatattt gtaggatgca ggatcaggag gataaaaaag gccatatttt
      241 gccacttctt cctgaaaaac aaaggtgata actcccggtt caactacagc gacaaggttt
      301 ttcgcgtcta tttccagaat tttgttcatt cttgtgaagg ttaaaacaac cccaccattg
      361 attggtattg aaccaccggt aagaccggat ccctggcccc ggggaatcac aggaaaccga
      421 tgcttgtttg caaggataag tatctgcgaa acctcctgtg cagaggttgg aaagacaaca
      481 agatctggga ttgtcccatc tgttcttgcg tcaaaggaat agcacttcct ctcctcatgt
      541 gatgtaaggg cattttcttt tccaacaatt tttatgattt cgtcaattat tgtttggtcc
      601 acaacaaccc tctttcaaat ctgtcaacaa tcgttctggt acagcatcaa gaaaacctct
      661 aaggatacta cattttcaca cttatctcaa aatgcttccc aatcttctgt gtcactggag
      721 tataatagac tttgaaggac atatttcctg acaacagaaa caggtaatac attggtcagt
      781 gtcaacttgt gggaatctgt cactcatttt cagggcagag acggggcact ggcccacaca
      841 ggtaccacaa cccgtgcacg atccctcatc aacacggggt tttagtagta cccgactgtt
      901 taaaaggttc tccattgccc tattgctatg gactgcttca cctccaagcg gcgggagttt
      961 aaagtcagga ataatcgtta tgtcccctac aatttctaaa tgattgagat cgtagtcacc
     1021 caaattgagc tcccttgccc gctggagaaa ccttagcctg cccggatcac agcccatcat
     1081 tgtcgccata acaccatcaa gggcaacagc attatctgat gcaaggacaa ggtttgtctc
     1141 tctcagatca gatgaagccg gcccattccc ttccatagcc acgataccat caacaataaa
     1201 gaaatcaggt actcgaattc ggaacacttc gactatcaag ttgttgaatc gttcaggatt
     1261 ccctgcagct ttgtgcaatc gagctttttg agctcccggt agaaaaccat agctattttt
     1321 tattcctcct gacaaaacag ttaacccgtg ggtcttaaac tttgggatgc tgatgataaa
     1381 atctgattcg agagcaaccc ttgaaatact gatggtggat aaaaagtccg gattaaagtc
     1441 aactttgatg gaatcggtgc caatattctg gtagtaacct tttgctgcct ccataagccc
     1501 tgtttttata aaggcatctt cattggcgcc atagctgaaa accccggagt tatctcccac
     1561 aatgattgat attggcttca tcatttccac ggtgtccact actgcccgca atatcatggg
     1621 atgggtagtg ataccctctt ccggctgtga tgccctgaga acattcggtt taatcagaac
     1681 cttcttccca cacagatcaa atggaaaaac ctcaaatgct tttttaatgg cttcagatac
     1741 agtttcgtat gtagcatgat ggatcaacac cttgtacata tacccctcag tgcaacaagg
     1801 cgtctaattg ttcttcgcaa atggtttcca tatggttgcc cttttacagg gtccgatggc
     1861 tcatggggtc atgcgccata ttaaggcgaa tcagttgtct ggatctttaa acaccgctcc
     1921 cgtagctgca cttgttacca tttttgcata tcgtgccatg taaccttcct tgattttcgg
     1981 ctgcggtttc ttccatgctc tctttcgctt ttccatctcc gccttattta catgaatgtc
     2041 tatcctctta tttacaaggt cgatctcgat tctatcgcca ttctttacga atgccacagg
     2101 gccaccttct gccgcctcag gcgatacatg cccgatacat aatccccttg tcccgccaga
     2161 gaatcttccg tcagttatca gcgcgcattg cgtatcaaga ccacggccag caagtatact
     2221 tgtgggggta agcatttccc gcatgcccgg accacccttg ggcccttcat atcggataat
     2281 gacagcatca ccctctttta ttttgccagc cataattgcc gcgcctgctt cttcctcact
     2341 gttgaacacc cttgccttac ccacaaaatg ccatgccttt tcctgaactc ctattcgctt
     2401 tacaattgca ccctgtgggg caagactccc agatagaact gcaaggccgc ctttttcatg
     2461 atacggtgca tcaagaggat gaataacctc cgggtttgtc actgctgctg tttttagaag
     2521 atcaccaagt ttttcccctg tcacattgag gcatttctta ttaatcagat ttttcttgga
     2581 cagttctttc atgatcccgt ataccccacc agcctcaaac agatcctcaa gatggtgtgg
     2641 gccggctgga ctcatattac aaatatgagg aaccttttct gatatttcat caaaaagact
     2701 gagtggcaaa ctgataccgc cttcgtgagc tattgctggc agatgcaggg ctgtattgga
     2761 tgatccacca aaagccatat caacggcgat ggcattcttg aatgcgtcaa gggtcataat
     2821 atcccgtggt ttcagatttt tctctaccaa ctccaaaacc ttctgccctg cagttttggc
     2881 gagccttatc cgtcctgcgt gcacagctgg aatagtgcca tttccaggaa ggcctatgcc
     2941 aagcgcttct gaaagacaat tcatggaatt tgccgtaaac ataccactgc aagaaccctg
     3001 tcctggacag gcacattgtt caagaagacc caactcctct tcagacatga gtccaccggc
     3061 aaccttcccc actccttcaa aaactgaaat gagatcaact ggtcttcctt taaatctacc
     3121 agcgagcatt ggtcccccgc ttatcaagat acatgggatg ttaagcctca aagcagccat
     3181 catcataccg gggataatct tgtcgcagtt tggaattagt accaggccat caaaagggta
     3241 agcttttgcc atgacttcca cggaatcgca tacaagctcc cgggatccaa gtgaatattt
     3301 cataccttca tgattcatgg caataccgtc gcaaatgcca atagtagaaa attccatggg
     3361 cgttccccct gccagcctaa taccatcttt aacggcctga gaaaccctgt gaaggtgaat
     3421 atggccagga atcagctcat ttgctgagtt agctacgcca ataattggcc tttcaagttc
     3481 atctggcagg taacccatgg cggaaaaaag gctcctatgg ggtgctttct caatcccttt
     3541 cttcatttta tctgatctca tacaggtctc ctttgcataa caacaggcat gtttcaaaat
     3601 tcatctgaca ctacaagatt attatacaca gcttcgcaat gcaatagtat aaataccggc
     3661 aagccatagg caataagcca aattattttc ccactcactt atcgcctttg acttttcatt
     3721 tctcgcttca tacttcgtct tcccgcccac ctctcacctc gcctttccca tttatatttg
     3781 ttagaataac gacatcatgt caggatttct accaacaaca aaagaagagg tggcaaagaa
     3841 ggggtgggac agccttgatg ttatcattat caccggcgat gcctatgtgg atcatccgtc
     3901 ttacggagca gcactcatag gaagggttct tgaaaatgca ggctatcgtg ttggcattat
     3961 tgcacagcct gactggcggt cgaaaaagga cttcatacgc cttgggaagc ctcgactatt
     4021 ttttggaata acagctggaa atatggattc catggttgcc aactacacgg caaacaagag
     4081 gcttcgtaaa aaagatgcat attcaccggg tggtaggcca ggtctaagac ctgacagggc
     4141 aacaatagtg tatgcgaacc gcgttcgaga agccttcgaa aatatccata ttgttattgg
     4201 tggcattgaa gccagcctca ggaggctggc acattacgac tggtgggaca atacagtgag
     4261 acggtcaatt ctccttgatg ccagagcaga tattctcgtt tatggtatgg gcgagaatca
     4321 ggtcaaggag attgcccata gggtagataa gggcattgat cttaccggta tcccaggcac
     4381 ggcaatcgtt cgaaagcatt taaacccgtt tgtcaatatg gaaatcattc atccttatga
     4441 agtcgtcaaa agttggacag atagatttaa tgaagcctat aaagcaatct actataatca
     4501 ggatccagtc aacggaagga ttgtggctca agcccatggt aacaggtttg ttattcaatt
     4561 tccaccggcc aggccactgg aaacccaaga attagacgct atttatgagc tacccttcga
     4621 aaaaaatcct cacccctcat atcaaaaaaa aggtattatt ccaggcttcg agaccacaaa
     4681 attctccata gtatctcacc ggggttgctg cggtgaatgc tatttctgct ctctattcat
     4741 gcaccagggg agaattgttc aatcacgcag cggatcatcg attctcaatg aggcacgctc
     4801 actttgtcaa aggcatgact tcaaggggac catatcagat gtaggcggtc ccactgtaaa
     4861 cctcttcatg gcagattgca ctttatggca caaatggggg gcctgcaaaa acagacattg
     4921 ccttattcca gagaaatgtc gtaaccttca tctgggttat gaggaaggtg tacaactcta
     4981 taaagagatt ctcaaaatcc cgaaggtaag gcatgttttt ctggaaagtg gttttcgaca
     5041 tgatttgttg gctaacaata aggcatcaag ataccttgag catctgtgca ggcatcacgt
     5101 aagtggtcgg ctgaaggttg caccggaaca caacatccct cacgtactca gattgatgaa
     5161 taaacctgat ttcaatattt acgaggcatt tgtgaggaaa ttccgggaaa tgaagaaaaa
     5221 aactggtaaa aaccagtacc ttgtgaacta ttttatcagc gcacatccgg ggtgtacgct
     5281 gcaagataca ctggagcttg ccatatacct ggccaagaga cgggtgcacc cagagcaggt
     5341 tcaagatttc attccactcc caatgacggt ttcaggatgt atgttctaca cagaaaaaca
     5401 tccttttacc ggtgaggatg tacatgtaac taaaacgctc cacgaacgta aaatacaccg
     5461 ggcactcatt caatacaaga atccatcaag taaaaaatat atccatgagg cattaaaaaa
     5521 acttggaaaa gaagatttgt cacgatattt cttacaaaaa tcttagcgta gttaaaaaaa
     5581 tgttttataa tggtatgtta aatttacaaa tttcatatga agagtactga aaacataaat
     5641 caacttatta ctgagctcga tacattgcgc aagcgggtat acgaactgga acaatatgag
     5701 gctgaacgta caatatgtga aaacacctca aaaaaaactg aagaagcctt tcttcaacta
     5761 ttcaactcca cagaagagat tgcttttctc caggagttgg acggtacgat tgtcattgca
     5821 aacgaaaaca gcaagcactt ttataagata ttagagggga atatagtagg gaaatcaatc
     5881 tttgaccttg tgcctgaaga tagggttgaa aatgctaaag aaaaagtgaa ggctgtcgta
     5941 gatacaaaga aaactgttcg ttttgagggc acccttggcc aaagtgtctt cgagaacagc
     6001 ctctatccag tctttgatca ggaagggaat gtacggcgca ttgcagtcta cgtgagagac
     6061 attacaataa gaaaacgtct cgaatcggct gttcaccagg cggaagagaa atacagaacc
     6121 atctacgaga acgctatgga ggggattttt cagactgacc tcgagggccg cttcatcagt
     6181 gcaaacccgg cattggccag catccacggg tatgactctc ccgaggaact cataaagacg
     6241 gttacagata ttccacacca gctctatgta aacccaactg actttaccaa tctatcgaaa
     6301 ctgctacata tccagggaga agctcaaggc gttgagacgc agatgtatcg gaaggatcac
     6361 agccgccatt ggatagttat caatgtccgt acagtaaagg acgaacaggg caaggtacag
     6421 tattatgaag gaacgatgct cgatgttaca aacagaaagc ttgcagagga acaactcaaa
     6481 gaaagcgagg aacggtaccg gatagctata gagcactcta atgatggtgt tgccattgta
     6541 cgaggcgaca ttcatgagtt tgtaaatcgg cggtttttag aaatgtttgg ctatgactcg
     6601 ctcgacgacc ttgtgggcaa aaaggttttt gtaattgttc atcccgacga tcgagaaaag
     6661 gttgttgaga taaaccgtcg aagacagact ggacagcccg tcccttcacg atatgaattc
     6721 aaaggcatta ccagagacgg cagggttatt tatgttgatg tttctgcggc cacaatcagc
     6781 taccgagaga atctcgtcta cctgacctac ctcagagata ttaccgaacg taagaaagca
     6841 gaggaagccc tgcgcaatga acgcaacaga ttccaggcgc tctcagatag tgcgcctttc
     6901 gggatcatgg ttattgatga acaaggcaac tttacttatt tgaaccccaa atttaccgag
     6961 ctcttcgggt atgacctgaa tgatgctccc agtggcagaa tttggtataa aaaagcattc
     7021 cctgacatta aagaacgggt agctgcaatg tcgggatggg taaggaatgt gaaaaacact
     7081 acacccggac agaggatagc aaagattttt acggttacct gtaaagatgg aacaaaaaaa
     7141 acagtcaact ttatccctgt acgattagca acaggggaat acctcgttag tcttgatgat
     7201 attactgagc gcattcaggc tcagaacgcg ttactgcaat cacacaaaga gcttgaaaac
     7261 ctcaatagag ccaagacgaa agcagtaaac cacatatctc atgaactcaa aactcccctt
     7321 gctgtaattc agggaaatat ccgcatcctc aagcgtaaga ttcagtcctt atctggggac
     7381 ggaagtttgg aaggaatcat tgcggttctt gaacgaaacc tggaacggct cttcggcatc
     7441 tccaaagaaa ctgatgagat attcagggta acccaggaag ttgaagcaaa tgtccttctt
     7501 gacgaccttg agcgtttatg ggaaagaatc gtagatcttt ctgaccttcc agaggatatg
     7561 caggagcatc tcaatgcatt aaaggaatgg gtaagtcagt acagggccgg cgggattctt
     7621 tcggcacagt cgatcgatct ttaccctttt attatgcaaa cccttgagaa agcaaaacat
     7681 tctgcacaag caagaaaaat tgattacacc ctcgagggtg aaaatgatct ctacatttcg
     7741 atggaccctg ttattttgcg ggaggttgca gaaggactca tcaagaatgc cattgaaaat
     7801 acccctgatg gaggaaaaat tgatatctcc gttgaacaga agggcgaccg aatatggtta
     7861 catgtaaaag actatggcat tggcatacgg gaagagaacc agcaatatct gttcgacgga
     7921 ctttttcatg cacacgaaac tgacctgtac agttcacgaa aaccttatga ttttggcgct
     7981 ggagggaagg gtctcgagct tctgcggatg aaagtctatg gacagcgcct cggttttgac
     8041 attaccatga aaagcaagcg atgcatctac atcccaacag accaggatgt gtgccctggg
     8101 gacgtaaccc aatgcaccca tataaccagc cctgaagaat gttatgcctc tggaggcaca
     8161 acattctctg tgtcattccc agcagagaaa aagcattctc aaacagaggt ctgatttttt
     8221 ccatgttctc tctcccaggc tgacagcaca catgcacaac agaatcagga acatttcatg
     8281 acaccatcag agttctcagt tgaaatcatt gacattgctt tgcccaacaa ctacggcgtt
     8341 gcaaaaaagg atggccgtgt tattttcgtt ccaggagcag tgattgatga caaagttacc
     8401 gtaagattaa accgtgaaag taaaaggttt gcttatggag agatttctca tgtggatgtt
     8461 ccatcatcat tcagggttac tccagagtgt cctcattttg gttcatgcgg tggatgcatc
     8521 atgcaaaact taagatatga caagcaactc ctcatcaaac aaaaatactt gttggataat
     8581 ttgaaccgtg tcggtggatt agatccacaa agcctggaga ttttaccagt tacaccatct
     8641 cccgatcttt atttttatcg aaacaagctt gagcttgctt ttggcaataa aaacgatgaa
     8701 gttatattag ggcttcggga aaggctatct cctttcaaac cattctctgc caatgttata
     8761 cccctcaata aatgtccggt attcagtact gtggcagaaa aggttattcc cattttgact
     8821 caatttgctc atgctgaagg atttacggct tttaatcctc tcaccaggaa aggaatttta
     8881 aaacacctta ttttaaggga gtcaaaatca acaggggaaa ttatggtcat tctcgaaaca
     8941 aaaacagaag cgcttcaagg cattaaaaat gttatccatg aaataggtca gcgggtgcct
     9001 ggagtggtca gtttttacca tgcaaccagc aggagaaccg atgatattat tcattttgac
     9061 aggatcaata ggctattcgg ggctcgttca atagttgaac acgtttccca tcttaatttg
     9121 aatatctacc ctcaatcgtt tttccaacca aattcaaaaa gcgctgctct cttgtacggt
     9181 gagatagcca atcaactcaa cctgaaaggt agtgaagtta tcctcggcct ttattcgggt
     9241 accggttcta tagaaatctt tctctctgat aaggccctgc aggtaattgg gatcgattct
     9301 gagcaggcga atatctcaac tgcaaataaa aactgcaagg ccaatcagat tgagaactgt
     9361 aaattttacc aatcacgggt tgaggatata ctgaagcaat gcaaactgcc gaaagctgac
     9421 atactgatca ttgatcctcc aaggaccggt ctgagcggac aagcgctcca tgttgtaaaa
     9481 cacgtcgcca ttccaaaaat tgcctatata tcatgcagcc caccaaccct agcccgtgat
     9541 ctgcgagaat tgtgtggcta tggatactca attcatcgaa ttattccctt tgacttcctc
     9601 ccccacacag ggcacctgga aattctcaca atccttgtgc attagccaaa gacagggatg
     9661 ttctctttat ctcgatttcg acattaagat aaacgtagca ctggatttct gttaactttc
     9721 tctttctttg aatttctgat aaaattggtc tggacaatct ttttaaagga taaaacttgg
     9781 aaaaaaaaga aattaaagag cgaatagaaa aactaaaggg tcaattggaa tatcataatt
     9841 accgatacta tgtccttgat gatccggtag tttcagacac tgagtatgac aggttaatga
     9901 cagaacttgt tgccctcgaa gaggaccacc cagaattttt aagtcccaat tctcccacgc
     9961 aaagagttgg cgcaaagcca ttggcagaat ttgaaaccgt cacccacaca attcccatgt
    10021 taagccttca gaatgctatg gagcttgatg aggtagcaga gttcgacagg cgcattaaaa
    10081 aacttcttag aatcaatgat gttgattacg ttatggaggt caagattgac ggcttggcag
    10141 tcgaacttgt ttaccttaac ggtgaattta ctacgggttc cacccgaggc gatggcttca
    10201 caggtgagga tattacccag aaccttcgga ctattaagtc cattcctatg aggcttttgc
    10261 gtgaccatga catcccgatt cctgacaggc tagaagtgcg tggtgaagta tatatgggaa
    10321 aaaatgaatt taaggaactg aataaggagc gtgagcttca aggggatccg ctttttgcca
    10381 atccgaggaa tgctggcgcc ggctcagtcc gccagctcga ccctagaata actgccggaa
    10441 gaaaactgaa tatcttctgc tatgcaccag gcgagattct cggggtcact ctgacaacac
    10501 attaccactt cctggattac cttaaaaaat ggggtttcag ggttaaccct ctgacaaaac
    10561 tctgccgcaa ccttgatgaa ttaatatcac attacaatta tattcagaac atgcgagaca
    10621 gaatccctta cgagatcgat ggaaccgtta ttaaagtaaa tcgctttgac tatcaggttc
    10681 ttctcggtaa cgtctcccgt tctcccaggt gggcaatcgc ttttaaattt caagctcatg
    10741 aagaaaccac gatcatagaa gatatcattg taggcgtggg acgaacaggt gccctcacac
    10801 ctgtggcagt gctgaaaccg gtgatggtaa gcggggttga agtaaaaaga gctaccctgc
    10861 acaatgaaga tgaaattgta agaaaggata tcctgattgg agatacagta atagtctcac
    10921 gagctggcga tgttattcct gaagtagtca aagtcataaa agaaaaaaga acaggccaag
    10981 aaaggccatt cacaatgcct gaagaatgcc ctgtttgcgg agagaaggtg gtaaggcagc
    11041 cgggagaagc aatccgtaga tgtgtgaata ttaactgccc tgcccaaatc aagggaagca
    11101 tcgagcattt tgcatcaaaa cgggcaatgg acattgatgg ccttggagaa aaactagttg
    11161 aacagctcgt ggacaaaaaa gcgataaacg acgtttctga cctctactat ctcaagaaag
    11221 atgatttgtt gcagcttgaa cgaatggctg acaaatccgc tcaaaatttg cttgatgcga
    11281 tcagtacatc aaaaaaacct tcttttcaga gatttatcta tgccctcggc atacgcaatg
    11341 tgggggagca catttctggg cttcttgctg acaaatttaa tagtattgag gatttgtata
    11401 ctcttgatga agatacactg atggctattc cagagattgg ccccgaggtc gcaaatagca
    11461 ttacatcatt cttccaagac gacaaaaaca gaaagacaat ttcaaggatg ctcgatgctg
    11521 gggttgtcat tgaatataaa aaagagggca gaaaacccct ccttggtctg acatttgttt
    11581 ttactggagc cctcaaaaat atgggtcgcg aagaagcacg aaaaaaggtt gaaagcctcg
    11641 gtggaaaaac tgtatcctca gtgagtaaaa aaattgatta tgtagtactc ggtgaagaag
    11701 ccgggtccaa ggttgacaaa gcccgcagtc tcgatctaaa gataattagc gaggaagagt
    11761 tcttaagctt aatacaaaaa tcttgactct tgaaaggcac gaatagtaaa tagattgtta
    11821 ggaaaccttg atgtcattca atatccatat tgaagaatag tctatgattg tatcacattc
    11881 aatactcatc acccattacg cgtcacggat tttattttga tcggtttcat cggcggaact
    11941 tcactcacta actcaactat cttcaagggc tggcatcaaa atagtattga gacaccctat
    12001 ggaactgtca ttctaaaaaa acgacaaagc tctgtctttc ttcaaagaca tggcagccct
    12061 taccttccgc ctcaccggat caatcatcac gcaaacattt gggccatgaa acacatcggc
    12121 gttgaagaga ttatcgctat taactcagtg ggaagcctga aacatgccat caaaccagga
    12181 acattcttaa tccctgatga ttttatttct atctggtaca ttccaacctt tttcaatgat
    12241 aaaatgcagt ttacagttcc ccgcatggac actgagtcag ccgatcgcct ttacaggcaa
    12301 tgtgagccat taaaaatacc tgtaaaaaaa ggtgggattt atatccagac ccagggaccc
    12361 cggcttgaga cgaaagcaga aatcggtatg cttaaaagat ttggcgacat tgtgggaatg
    12421 actatggcat cagaggttac tttgtgtctt gagtgcggca ttccatatgc gagcatatgt
    12481 tcagtagaca attactgcaa cggaatcaca aaaataccac tcaccatgga tgaaattcag
    12541 aagaatgtac ggaataatct gcagaacatt gaaaggttga tcgactcgat tcaccagggg
    12601 agattataat gactattctg attaaaagcg tgatggtgaa tggaaaaatt caagatattt
    12661 tcctggaaga cggagtggtt tgcgaaatct cggatgagtg cataaaaata gcagaccgtg
    12721 ttatatatgg acaaaacaaa gctgccctcc cctcttttat caacgggcac actcatgctg
    12781 caatggctct tttcagaggc tacgccgatg acatgcctct gaaaagctgg cttgaggaaa
    12841 agatatggcc ccttgaaaaa aaactgaccg aagaagatgt ctattggggt acaaagctcg
    12901 catgcctcga aatgataaaa aacggaataa ccgtattcaa tgacatgtac tggcaatgga
    12961 aagcaacggc aatggctgtc aaagagatgg gaatcagggg gtttctcagt gcggtcttca
    13021 ttgacatgtt tgaccaaaac aaaagtgctg aagagattga tcttaatgta agactctttg
    13081 aagtgtcgaa acaattccaa ccagcggtta catttacctt tggcccacat gcgctctaca
    13141 cagtgtccag ggaaagcctt gaatggatac gtgacttctc ccatgcggaa aacatcctca
    13201 tccatatgca cctttcagaa acagaggatg aaattcattt tgtaaaagaa aaatatggct
    13261 tatcacctac cgaatttctc cattcaatag ggattctatc tgaccgtttc attggttgtc
    13321 atggctgctt tcttgataaa aatgactgca gcattctcaa aagaaacggg gcaaagttgg
    13381 tccatgtgcc tgtttcaaac ctcaaactgg cagtaggacg aatttttccc tacgctctcg
    13441 cgaaagaata cgggatacca ttctgttttg ggactgacgg atgtgcgtcc aataatgatc
    13501 ttgacctgat tgaaaccgtg aagtttgctg ccctacttgc caagttttcc acccataatc
    13561 caaccatgat gccggcaaag gaagctttta accatgctac taaaactgca gccgctattt
    13621 ttggcatggg tgagtgggaa ataaaagttg gagcgagccc tgatatcatt ctcgtcaatc
    13681 ttaactgtcc agagatgaca ccagactttg acctttattc caatctggtt tatgcatcga
    13741 agggctcaat tgttgatacg gtaatatgta tgggtaaggt cattatggaa aatcgcacgg
    13801 tacccggaga ggaagaaatt ctcagaacca cctcaaaaat cgcaaaatca ttggccaaca
    13861 gatgaaattc atttgcgatg ccatgctcgg aaaactcgcc aagtatttaa gaatccttgg
    13921 ttttgatgct gagtacacga aagattcgaa aattcttgaa cattacaaag gaaagactga
    13981 tgctccgtat tttttgacac gcacgatcaa aaaaggtggt tatgaacgga caattgttat
    14041 cctgtctgat aaggcaagag agcaattgcg cgaaatcaga gaacttatcc atccgtttat
    14101 tgattccgcc aaagtcatga accgttgtat tgaatgcaat attgacctta tgggtgtgca
    14161 gaaaaaacat attgaacaga aggtaccgga atttgtttat caccagtatg aacaattcaa
    14221 agagtgtcct cgatgtagaa aagtgtattg ggaggggaca cacacagcag ggatgacaga
    14281 gctaatccga gaaattatgg aacatgctga tatgaaagct gaaaaaacag tccaggcaga
    14341 aaagatgtaa aaagtaaaaa aggtttgagt caaaatggag aaacctgaaa gagttcacac
    14401 ctatggaaga agatgcaaaa tcagaggttt catgcttata acttgtgctc
//
LOCUS       DAOOMC010000076        14429 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdus sp. isolate AMR_MDS_2685
            NODE_4508_length_14429_cov_6.148463, whole genome shotgun sequence.
ACCESSION   DAOOMC010000076 DAOOMC010000000
VERSION     DAOOMC010000076.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864760
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdus sp. (wastewater metagenome)
  ORGANISM  Syntrophorhabdus sp.
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae; Syntrophorhabdus.
REFERENCE   1  (bases 1 to 14429)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14429)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,729
            Genes (coding)                    :: 2,708
            CDSs (with protein)               :: 2,708
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 7 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14429
                     /organism="Syntrophorhabdus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4508_length_14429_cov_6.148463"
                     /isolate="AMR_MDS_2685"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2699756"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..357
                     /locus_tag="PK125_08720"
     CDS             <1..357
                     /locus_tag="PK125_08720"
                     /inference="COORDINATES: protein motif:HMM:NF014874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanophycin synthetase"
                     /protein_id="HPB38231.1"
                     /translation="TRLKKGRLILVFGCGGDRNRAKRPVMGEIASHLTDLTIITSDNP
                     RSEEPISIIEDIRKGFSGNSFKIIENRHDAIYEAVQIAKEGDILLVAGKGHEDFQIVG
                     SKVFHFSDRETIEESL"
     gene            357..1715
                     /gene="murF"
                     /locus_tag="PK125_08725"
     CDS             357..1715
                     /gene="murF"
                     /locus_tag="PK125_08725"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR01143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HPB38232.1"
                     /translation="MWRVEDIIQAVHGEAYRIERGIFNDISTDSRTIQEGELFVPING
                     KTFDGHEFIVDAYLRSHGGTLCEKGREDVIKAAKGTIILVDNTTKAFLDLARHMRKSL
                     KGTFIAITGSNGKTTTKEILVAILGRSRQVHFNEKNFNNLIGVSKSILAVKGSPEMFI
                     FELGTNSRGEIRQLTEVTEPDVSVITNINPSHLEGLADLDGVFDEKSDIFRHTKAGGH
                     ILINADDPMLFPRYQDSDHVYHTFGIDGVADFHLRIREDLGWKGYDLTLEFFKDTFNV
                     KTRLLGRHNLYNILVASSIAYLVGISPSQIKSVIEAFNPYSMRFNPIMGKKAYTVVDD
                     SYNANPSSVKWALNTLASLPCTGRRIAVLGDMKELGEKTAFYHEEIGRFLKESNINSV
                     LLIGEEVKGILKELNNGRGRFFHDKASLIDYAKQYLTGGDVVLIKGSRAAKMEEIVEA
                     LI"
     gene            1716..2795
                     /gene="mraY"
                     /locus_tag="PK125_08730"
     CDS             1716..2795
                     /gene="mraY"
                     /locus_tag="PK125_08730"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR00445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HPB38233.1"
                     /translation="MLYHLLYPLNTTFSVFNVFRYITFRTALAILTALVISFFLTPNL
                     IKKFNDWKLKSEKREDVPERHEAKKGTPTMGGFIILVSTIIPTLLWADLKNSYIWVVT
                     FALLSFGAVGFVDDFTKLRRKQNGKGISGVSKLILQLLFGITIAIFLYQRDDFITQLT
                     IPFFKNATPDLGIFYILLCIFIIVGTSNSVNLTDGLDGLAIGPVLTVCSTFLLFAYLV
                     GNIKFAQYLQIFYVKGAGELTILCGAMLGAGIGFLWYNAYPAELFMGDTGSLSLGASL
                     ATIAVIIKQEFLLVIAGGIFVMETFSVIIQVLSFKWRGKRVFKMAPIHHHFELKGWNE
                     EKIVVRFWIISIMLGLFALTTLKLR"
     gene            2886..4211
                     /gene="murD"
                     /locus_tag="PK125_08735"
     CDS             2886..4211
                     /gene="murD"
                     /locus_tag="PK125_08735"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HPB38234.1"
                     /translation="MNLPDDILIVGLGMTGVATARFLSQMGKKITIVDEKPEQDLFVS
                     LKELEDIRFIGHFGSHRREDFLDHSLIIISPGVDSELSLLKEAREKGIKVIGEMELAS
                     RFIVEPIIAITGTNGKTTVTTIIGEMFKKAFGNIFVGGNIGDPLINYVLKGAKARALI
                     AEVSSFQLETIETFRPQTSILLNITEDHLDRYRSFSDYKDAKYRIFENQQQEDYAILN
                     KNLPDTDGIQAKRLFFSSKDEIKEGSFYQDGLMIVRLQGQEYRYERDASPLIGVHNTE
                     NLLVALLVAHIHGIEKDCIEEALKEFKGLPHRIEAVRRIDNILFFNDSKATNVDSTKR
                     ALESVEGNVVLIAGGKDKGGSYRALESQMKKVRALILIGDAKERIKAELGKYSETHLE
                     SDLSSAVKRALQIAKDGDVVLFSPMCSSFDMFKSYKDRGNQFRAMVESL"
     gene            4208..4993
                     /locus_tag="PK125_08740"
     CDS             4208..4993
                     /locus_tag="PK125_08740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB38235.1"
                     /translation="MKKAIIYILASMLLYSFFYEIDTMKMIFILLGLISVFAISHVPI
                     RVVMGAKYPVILLSFAGTAGFLVYPQLAVSYPVEPLIIFFALYSIAFYLVTLEEKGQD
                     VYKEIGGLSVLFISASFNLAMTGKPVLILPLALTIISFLFVLGRHKTILLMAGYTLAI
                     IIVLVYKHIPVLSTGVPVREINRYILFATSFVFLAISFIGFAKKGGQVKVLTFFGFLY
                     VCIDLLLSTLLKMSEGLLYQPVISLFMISPLIGLMVKSEGKRI"
     gene            4990..6051
                     /gene="murG"
                     /locus_tag="PK125_08745"
     CDS             4990..6051
                     /gene="murG"
                     /locus_tag="PK125_08745"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: protein motif:HMM:TIGR01133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HPB38236.1"
                     /translation="MKLLISAGGTGGHIFPGIAVAESFTGMHESNDVVFVGTQQGLEG
                     GIIPRYGFRLLFIEAHQFQGKNMFSKISTLVRLVQGIRAAKVIINLEKPDAILGMGGF
                     TCVPTILAGVMLGLPTFLHEQNVEPGLANKFLSRFVRSTFISFKATEKYLGTKNIFHT
                     GNPLRKTLKVAKVPEEKHTFNVFIFGGSRGARSINDSILTLLPFMESYKNTVMYHQTG
                     TEDYEHIKKVYEGSKVEYEVFPFTDNMEKYYNLSDVVISRSGATTIFELAYFKKAAIL
                     IPYPFSAGQHQKTNAAHVASLGGAYVVDNDELNGKKLHNLLSSLMENPGRLKEMGNNI
                     GRLYIEDAAERILKEIVGF"
     gene            6052..7428
                     /gene="murC"
                     /locus_tag="PK125_08750"
     CDS             6052..7428
                     /gene="murC"
                     /locus_tag="PK125_08750"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR01082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HPB38237.1"
                     /translation="MVFHKIDKVHFVGIGGIGMSGIAEVLLNLGFKVTGSDLRTTETT
                     ERLQALGAKIFTGHKEENIGGADVVVVSSAVRPDNPEVQKAKELFVPVIQRAEMLAEL
                     MRIKYSIAVAGAHGKTTTTSLVSTILAHAGLDPTCVIGGRLNSLGSNAKLGGSKFLVA
                     EADESDGTFLLLFPTIAVTTNIDLEHLDFYQDINEIKAAFLSFLNKVPFYGLDIICID
                     NANVQSLIPQLKRRYMTYGLSKQADLRAENISYDGFITSFKVLYKDKEMGDIVLALPG
                     IHNVVNALAACGVAIELDIPFRKVREALEGFGGIHRRLEMKWNGAIKLIDDYGHHPTE
                     IKATLAAVRQMWKGRIVVVFQPHRYTRTKALMEEFATSFNEADVLIVTEIYAASEEKI
                     EGVDGQGLSETIRASGHKNVMFAASKEDAAEVVLGMAREGDVVITLGAGDICRIEERL
                     KAAWSGQG"
     gene            7455..8306
                     /gene="murB"
                     /locus_tag="PK125_08755"
     CDS             7455..8306
                     /gene="murB"
                     /locus_tag="PK125_08755"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: protein motif:HMM:TIGR00179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HPB38238.1"
                     /translation="MKRYTSMRVGGPVSYLIYPCDEEDLVTILQVAWEEGVKYRFLGN
                     GTNIIVHDRGLREVVIRTTRMQRVAYKKKNNGAIAQVSGGASLKGFIVNSARHGLSGL
                     ERLYWIPGTIGGGIKMNAGSFGSRISDLLESVRIVDKKGKIVSYLKSEMRFGYRESRI
                     GPSECVLAAWFCLQNRDKNEIWKDMEYVYGERKKRHPMDFPSAGSVFKSVEGKPAWKF
                     IEKAGLKGTSVGGAMVSDKHANFIVNAGGATAQDVKLLIDKIKQEVFEQSGITLEEEV
                     EFWGFNG"
     gene            8299..9207
                     /locus_tag="PK125_08760"
     CDS             8299..9207
                     /locus_tag="PK125_08760"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: protein motif:HMM:NF002378.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HPB38239.1"
                     /translation="MDREVLKAQRVGVLMGGRSSERDISFKSGKAVIQGLKRRGYNVT
                     AIDVDPDLAIKLKRKHIGAAFIALHGRWGEDGSVQGLLEIMGIPYTGSGVLGSAMAMD
                     KVVMKMMFESMGIPSPAYTQAEDGGTVHFPLPFVVKPANEGSTIGISIVKKQKEVMAA
                     IKKARMFDKKVMVEKYIEGDEITVGIVNGEALPVVQVKPSSGFYDFEAKYTKGMTEYI
                     VPAKIDITIAKKAGDIAMEVYKVFELAGCVRIDMLVDDDLPKVIDINTSPGMTETSLV
                     PKAWESLGRTFDELVEAILMEASLKI"
     gene            9204..9911
                     /locus_tag="PK125_08765"
     CDS             9204..9911
                     /locus_tag="PK125_08765"
                     /inference="COORDINATES: protein motif:HMM:NF015739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HPB38240.1"
                     /translation="MKKSLYTLFLIPLCVISMLTVIYVFAKDEPLFFLRNIKVNGIQH
                     LKDHDIMGRAAPFLRDSLFKIDISEMKDAILTHPFVREVRIKRVYPFSLVIDVDEKKA
                     SALWADSEGQLHVLDDNGEPFRGFSREDSKGLIVINAKDKNGAKAVYRQINEWFREGI
                     IKKDDLSDVAYSEGNITIYGCGCDDGVEIILGKEDYTSRLKKAVAVLQDAKQRGFLIK
                     CIDARFEKGAIIKERQG"
     gene            9913..11157
                     /gene="ftsA"
                     /locus_tag="PK125_08770"
     CDS             9913..11157
                     /gene="ftsA"
                     /locus_tag="PK125_08770"
                     /inference="COORDINATES: protein motif:HMM:NF007009.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HPB38241.1"
                     /translation="MVTEDGLLVGLDIGTTKICVVVAKITEGKVNIVGIGSHPSTGLR
                     KGVVVNMDSTVNSIKKAVEEAELMAGIKIDSCLVGIAGAHIKSFNSNGVVAIKDKEVR
                     PDDVARAIDAAKAVAIPADRELIHVIPQEYIIDDQDGIRDPIGITGVRLEVKTHIVTG
                     SVSSAQNIVKCCKLAGLNVDDIILGQLASSEATLTPEEKEIGVALVDIGGGTSDIAVF
                     INGAIKFTSVLPYGGNNITNDIAIGLRTPLEEAEKIKKKYGCAFANMIGANETIEVPS
                     VGGRKPRTLMRKTLADIIEPRVEEISSLIYQEIKKSGAERLLASGVVITGGCANLEGI
                     AELAENIFNLPARRGYPIGLGGLVDVVNNPIYATGVGLLVYGFKNSRVRKHGFKKGTR
                     FKDFLNSRKLLYRMRDWFKEIF"
     gene            11169..12326
                     /gene="ftsZ"
                     /locus_tag="PK125_08775"
     CDS             11169..12326
                     /gene="ftsZ"
                     /locus_tag="PK125_08775"
                     /inference="COORDINATES: protein motif:HMM:TIGR00065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HPB38242.1"
                     /translation="MSNVFFMDETNGFSAKLKVVGVGGGGCNALNNMVEAGVQGVEFV
                     AINTDVKSLSMCKAPVKIQIGSKLTRGLGAGADPEVGKKAALEDVEKIRDHLAGTDMV
                     FVTCGLGGGTGTGASPVIAEISKELGALTVAIATKPFAFEARARMEQAERGVAQLRNS
                     VDSLITIPNQRLLSIGGKHMTVMEAFFKADEVLLNAVRSISDLIVGSGYVVVDFADVK
                     TIMAERGMAIMGVGMASGENRARDAAQKAISSPLLEDISIHGARGVLINVTGNTDITL
                     AEVNEASTLIQEQAHENAKVIWGLVFDDSMDDSVRITVIATGFEEQIISESDNVESFP
                     RSKLFVEDENVPPFQRKKIAIDYKEIKARSDNIDIDDDRLDIPTFLRKQAD"
     gene            12638..14122
                     /locus_tag="PK125_08780"
     CDS             12638..14122
                     /locus_tag="PK125_08780"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HPB38243.1"
                     /translation="MSNLALHILYTTLNSHSDIVCERCFFDEKEQLRSFESGKPLTSF
                     DLVFITLSFELDFVNIPKILNCGRLAVLASERKEGDPIVVAGGICVMANPEPVSKFID
                     LFILGDIEATVPLFIKKYLDTTGKNRHDVIQALSGFKWVYNPVALDVTYASDGTIGDF
                     KPHHHFITIENYRGKNLGSSAIITRETEFANMFLVEGTRGCPSQCPFCLLGNMYPFNY
                     DRVERFHPDIEDIGIIGGGVSYHPRLVDIIKAMKKEGKHIHLPSLRVDETTTQLVELI
                     QDEIKTLTFGIEAGSERLRKFIGKPLTDDEIYEKLETILVIKPFNLKLYFMIGLYGED
                     KKDLEDIISMVKHIHHIMVKTGAPKGIMGSITVHVSPFVPKAFTPFQWLPMEEMDALK
                     GKIAWIKKGLTRVGNTYFTHESVKYSVVQGILARGDRRVGDVILRLARGDSFNKVMRE
                     SPLNLNFYVHRERNKDELFPWDFIKGRQTKSALWKRLGSLKCSL"
     gene            complement(14219..>14429)
                     /locus_tag="PK125_08785"
     CDS             complement(14219..>14429)
                     /locus_tag="PK125_08785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB38244.1"
                     /translation="SCHIQETQNNSVRFLRLHLHNYGVFEGPMTLCLTVSGFREKLLR
                     REEARSFTGAKVPKLNTSKWSRWSE"
BASE COUNT         4183 a         2814 c         3573 g         3859 t
ORIGIN      
        1 acccgtctaa agaagggaag gttaatctta gtgtttgggt gcggtgggga caggaacagg
       61 gcaaagaggc ccgttatggg tgaaattgca tcccatttga cagatctgac cataataaca
      121 tcagataatc cacggagcga ggagcccatt tcgattatcg aagacatcag aaaaggtttc
      181 tcaggtaatt cgtttaaaat catagagaac agacatgatg ctatctacga ggcggttcag
      241 attgcaaagg aaggtgacat cctgcttgtc gccggtaaag ggcatgagga tttccagata
      301 gtaggcagta aagtgtttca tttcagtgac cgggaaacaa tagaggagtc tttgtaatgt
      361 ggcgcgttga agatattata caggcggttc acggagaagc atacaggatc gaacgaggta
      421 tatttaatga catctccaca gattcacgga ctatccagga aggagaactt tttgtaccaa
      481 ttaacggtaa gacatttgat gggcatgagt ttatagttga tgcctacctc agatcacacg
      541 gtggaacact gtgcgaaaaa gggcgtgaag atgtaatcaa ggcagcaaaa ggaaccataa
      601 tcctggtcga taacaccacc aaggcattcc tggatctcgc tcgccacatg aggaagtccc
      661 tgaaaggaac ctttattgcc attaccggga gcaatggcaa aacaaccacg aaggaaatac
      721 ttgttgccat ccttggcagg agcagacagg ttcattttaa tgaaaagaac ttcaacaacc
      781 tcataggggt ttcaaaaagt atccttgctg tgaaaggcag ccctgaaatg tttatctttg
      841 agcttggaac aaacagcagg ggggagattc gccaacttac agaagtgacc gaacctgatg
      901 tatcagtcat tacgaacatc aacccctctc acctagaggg cctggccgat cttgacggtg
      961 tttttgatga gaaatctgat atcttccgac acacgaaggc tggcggtcac attctcatta
     1021 atgctgacga cccgatgttg tttccacgct accaggattc tgatcacgta tatcacacct
     1081 ttggtattga cggggttgct gatttccacc tccgtatacg tgaggacctt ggttggaaag
     1141 gctatgatct caccctcgag tttttcaaag acacatttaa tgtgaagaca aggcttttgg
     1201 gaagacataa tctgtataac atactggttg cttcgtcaat tgcctacctt gtgggaataa
     1261 gcccgtcaca aataaaatca gttattgaag cattcaatcc atattctatg cgattcaacc
     1321 caatcatggg aaaaaaagca tacactgttg ttgatgattc atacaatgca aatccttcct
     1381 ctgtaaagtg ggcgctcaat acccttgcat cccttccttg cacaggcaga agaatagctg
     1441 ttcttggaga tatgaaagaa cttggagaaa agacagcatt ctatcatgaa gaaatagggc
     1501 gatttctcaa ggaaagcaat ataaacagcg ttctgttgat cggcgaagaa gtgaagggaa
     1561 ttctcaagga actgaataac ggacggggaa ggttttttca tgacaaagca tcactcattg
     1621 attacgcaaa acaatactta acaggggggg atgtggttct tatcaaaggt tcacgggcag
     1681 caaagatgga agaaatagtg gaggctctga tctaggtgct ctaccatctt ctctaccctc
     1741 ttaatactac attttcagtt tttaacgtct tcagatatat aacctttaga acagctctgg
     1801 ctatcttgac tgcccttgtc atcagttttt tcctgacccc caatctaatc aagaaattca
     1861 acgattggaa attaaagagt gaaaaaagag aggatgtacc agaacgacac gaggcaaaga
     1921 aaggtactcc caccatgggg ggctttatca tcctcgtgtc aaccataata ccaacccttc
     1981 tctgggcaga tcttaaaaac agctatatct gggttgtgac ctttgcactc ctttccttcg
     2041 gtgcagtcgg ttttgtggat gacttcacaa agctcaggag aaagcagaat ggtaaaggaa
     2101 tttccggtgt gtctaagctt atactccagc tactgttcgg tatcacgata gccatattcc
     2161 tgtatcagcg agatgatttt atcacgcagc ttaccatccc gtttttcaag aatgctactc
     2221 ccgatcttgg cattttctat attctgctct gtatcttcat tattgtcgga acctcgaaca
     2281 gtgttaatct tactgatggt cttgatggtc ttgcaattgg acctgtgctg accgtgtgct
     2341 caacgttctt gctcttcgcc tatctcgtgg gaaatattaa gtttgcccaa tatctgcaaa
     2401 tcttctatgt taaaggagca ggagagctca cgatcctgtg tggggctatg ctcggggccg
     2461 gcattggttt tctctggtac aatgcctatc cggcggaact ttttatgggc gatacgggtt
     2521 cgctctccct tggagcctcc cttgctacca tagcggtcat tataaagcag gaattccttt
     2581 tggtcatagc cggcggtatt tttgtcatgg agaccttctc cgtgattata caggtcctat
     2641 cgttcaaatg gcgcggtaag agagttttca aaatggcgcc cattcatcat cactttgaac
     2701 tcaaaggttg gaatgaggag aagatagtag tgcgtttctg gattatctcc attatgttag
     2761 ggctttttgc ccttacaacg ttaaagctga ggtaggagtg gaacaggttc aaggttatag
     2821 gttcaagatt tcaaaaaagg tcatgaagca ggaaacgttt ttgacgaaaa cggagttgcg
     2881 ctggtatgaa tttgcctgat gatattctca ttgttggcct tgggatgaca ggtgtggcaa
     2941 cagcgcggtt cctgtcgcag atggggaaga agattaccat cgttgatgaa aaacctgaac
     3001 aggacctttt cgtctccttg aaagaattag aagacatcag atttataggt catttcggct
     3061 ctcacaggag agaagatttt cttgatcact ctttaatcat tatcagccct ggtgtggaca
     3121 gtgaactttc attgttgaaa gaagcaagag aaaaaggaat aaaagtgatc ggagagatgg
     3181 agcttgcatc taggtttatc gttgagccta tcatcgctat aacgggaacc aatggaaaaa
     3241 caacggttac tacaatcatc ggtgaaatgt tcaagaaagc gttcggtaac atatttgttg
     3301 gtggcaacat cggagaccct cttatcaatt atgttctgaa gggtgccaaa gcaagagccc
     3361 tcattgctga ggtgagcagt ttccagttag agactataga gacattccga ccacaaactt
     3421 ctatcctgct taatatcaca gaggatcatc ttgatcgtta tcgaagtttt tccgactaca
     3481 aagatgcaaa ataccggatt tttgaaaacc agcagcaaga agactatgca atcctcaata
     3541 agaaccttcc tgacactgat ggcattcagg caaaaaggct tttcttttct tccaaagatg
     3601 agattaagga agggtctttt tatcaggatg gattgatgat agtcaggttg caaggacagg
     3661 aatatcgata cgagagagac gcatctccgc ttataggtgt tcataacacg gaaaatcttc
     3721 ttgtagcgct ccttgttgcc catattcacg ggatcgaaaa ggattgtatt gaagaggcgt
     3781 tgaaggaatt taaagggctc cctcaccgta tcgaagcagt tcgcaggata gacaatattt
     3841 tgtttttcaa tgattcaaaa gctaccaatg tggactcaac caaacgagcc cttgaaagcg
     3901 tagaaggaaa tgttgttctc atagcagggg ggaaggataa aggcgggagt tacagggcct
     3961 tggaaagcca gatgaagaaa gtacgagccc tcatcctaat cggggacgca aaagaaagga
     4021 ttaaggctga acttggcaag tactcagaaa cgcatctgga gagtgatctt tccagtgctg
     4081 taaaacgtgc acttcagatt gcaaaagatg gtgatgtggt gcttttttcc ccaatgtgca
     4141 gcagttttga catgttcaag agttataaag accggggcaa tcaatttaga gccatggtgg
     4201 agtcactgtg aaaaaggcca ttatatatat tctcgcatcc atgcttctgt atagtttttt
     4261 ctatgaaata gacacaatga aaatgatttt catacttctt gggctcatct cagtttttgc
     4321 catttctcat gttccaataa gggttgtcat gggagcgaaa tatcctgtca ttttactttc
     4381 ctttgcaggg actgcaggtt tcctggtcta tccacaactg gcagtctcct acccggtgga
     4441 acccctcatt attttcttcg ccctgtacag tatcgccttt tatctggtca ctttggaaga
     4501 aaaggggcaa gacgtgtata aggagatagg agggttatcc gtacttttta tttctgcttc
     4561 cttcaatctt gcgatgaccg gaaaaccggt cttgatatta cctctcgcac ttaccattat
     4621 atcctttctt ttcgttctgg gaaggcacaa aaccatcctt ctcatggcag gctacactct
     4681 ggccatcatt attgttcttg tgtacaaaca tattccagtt ctttctaccg gtgttcccgt
     4741 gagagaaatc aaccgttata ttttgttcgc aacatccttt gtttttcttg caatcagctt
     4801 tattggattt gcaaagaaag gaggacaggt caaggtactt acattttttg gattcctata
     4861 cgtatgcata gacctcctcc tgtcaacctt actcaagatg tcagaaggat tactatacca
     4921 gcctgttatt tcgcttttta tgataagccc tcttatcggc ttaatggtta aatcagaggg
     4981 gaaacggata tgaagcttct tatctctgcg ggagggacag gaggccatat ttttccaggg
     5041 attgctgtag cagagtcctt tacagggatg catgaaagca atgatgttgt ctttgtcgga
     5101 acacagcagg gactagaggg tggcatcata ccgagatacg ggttcagact cctttttatc
     5161 gaagcgcatc agtttcaggg taagaatatg ttctcaaaaa tctcaaccct tgtgcgcctt
     5221 gtccagggta tacgggcagc caaggttatc ataaacctgg agaaacccga tgcaattctc
     5281 ggtatggggg gcttcacctg tgttcccact attcttgcag gtgtaatgct cggattgcca
     5341 acctttcttc atgagcagaa tgtggagcca ggtcttgcaa acaaattcct gtccaggttt
     5401 gtgcgatcta cctttatcag cttcaaggca acagagaaat atcttggaac aaaaaatatt
     5461 ttccacactg gcaacccgct cagaaagaca ctcaaggtag cgaaagtgcc tgaagaaaaa
     5521 cacactttca atgtatttat attcggtgga agtagaggag cgagaagcat taatgattca
     5581 atccttaccc tccttccgtt tatggagagc tataaaaaca ctgtaatgta ccatcagacc
     5641 ggcaccgaag attatgaaca tatcaagaaa gtatatgaag gttcaaaagt ggaatatgaa
     5701 gtttttcctt ttacagacaa catggagaaa tactacaatc tttcagatgt agttatatcg
     5761 aggtctggcg ctaccacaat ctttgagttg gcttacttta aaaaggctgc tattcttatt
     5821 ccttatcctt tttctgcagg tcaacaccag aagacaaatg cagcacacgt ggcaagtctg
     5881 gggggtgcgt acgttgttga taacgatgaa ttaaacggta aaaaactcca taatttgctt
     5941 tccagcctca tggagaaccc tggccgtttg aaggaaatgg gtaataacat tggcaggctt
     6001 tacatcgaag acgctgcaga gcgtatctta aaagaaatag tggggtttta gttggtattc
     6061 cataagatag acaaggtcca ttttgtaggc attggcggga tagggatgag cggtatcgct
     6121 gaggttcttt taaaccttgg tttcaaggta acaggttctg atttgcgcac aactgaaaca
     6181 actgaaaggc tgcaagccct gggtgcgaaa atttttacag ggcataagga agagaatatc
     6241 ggcggtgctg acgttgtagt ggtatcgtca gcagtgaggc ctgataatcc ggaagtccag
     6301 aaagcaaaag aactttttgt tcccgtcatt cagagggctg agatgctcgc agaactgatg
     6361 aggataaaat acagcattgc cgttgcaggg gcacacggga aaacaacaac cacatccttg
     6421 gtatcaacta ttctggctca tgcaggactc gaccctacat gtgttatcgg gggaagatta
     6481 aacagcctgg ggagtaatgc caaactggga ggcagcaagt ttctcgttgc agaagccgat
     6541 gagagtgacg gcacatttct tcttcttttc ccaaccatcg cggtgactac caacattgac
     6601 ctcgaacacc tcgattttta tcaagacata aacgagataa aagcagcatt tctctccttt
     6661 ctcaacaagg tacctttcta tgggctcgat attatctgta tcgataatgc caacgttcaa
     6721 agtcttattc cccaactcaa gagaagatat atgacttacg gtctttcgaa gcaagcagat
     6781 ttgcgggcag agaacatttc ctatgacgga ttcattactt catttaaagt tttatacaaa
     6841 gataaagaga tgggggacat tgtccttgct ctccctggta tccataatgt ggtaaatgcc
     6901 ctcgctgcat gtggggttgc catagagctt gatataccgt ttcggaaggt cagagaagca
     6961 ctggaaggtt ttggtggaat tcataggcgt cttgagatga agtggaatgg tgcaataaaa
     7021 ttgattgacg actatggtca tcatccaaca gagatcaaag cgactcttgc tgctgttcgg
     7081 cagatgtgga agggacggat tgttgtggtt tttcagcctc accgatatac acggacaaaa
     7141 gcactcatgg aagaatttgc aacttctttt aacgaggccg atgttcttat tgtgactgaa
     7201 atatatgcag cttcagaaga gaagattgaa ggggttgacg ggcaggggct ttcagaaacc
     7261 atcagggcaa gcggccacaa aaatgtgatg tttgctgcta gcaaggaaga tgccgcagag
     7321 gtagtgcttg gcatggcccg ggagggagat gtcgtgatta ccctaggcgc cggtgacatt
     7381 tgcaggatag aggaaaggtt aaaggcagcg tggagtgggc agggataaga ggatcgattc
     7441 tgaaggacgt ttcaatgaaa aggtacacat ccatgagggt tggtgggcct gtcagttatc
     7501 tgatctaccc ctgcgacgaa gaggatttgg tgacaatttt acaggtggca tgggaggagg
     7561 gtgtcaagta tcgattcctt gggaatggaa caaatatcat tgtccatgac agggggcttc
     7621 gagaggtagt gataaggaca accagaatgc aacgtgtggc atacaaaaaa aagaacaacg
     7681 gtgctattgc gcaagtgtca gggggagcat cgctcaaggg ttttattgtc aatagtgcac
     7741 gtcatggttt gtctggcctt gaaaggcttt attggatacc aggtaccata gggggcggta
     7801 tcaaaatgaa tgcaggaagt ttcggttcga gaatatcgga tttgttggag agtgttcgta
     7861 ttgttgataa aaagggaaag attgtctctt acctgaagag tgaaatgagg tttgggtatc
     7921 gagaatcacg gataggtcca tccgaatgtg tccttgcagc ctggttttgt ttgcagaaca
     7981 gagataaaaa tgagatctgg aaagatatgg aatatgttta cggtgagcga aaaaagaggc
     8041 atcctatgga ttttccctct gcaggttctg tgttcaaaag cgttgaaggt aaacctgcct
     8101 ggaaatttat tgaaaaagct ggcctgaaag gaacctctgt tggtggagcc atggtttcag
     8161 ataaacatgc aaattttatt gttaatgcag gaggggcaac agctcaggac gtgaagttac
     8221 ttatagataa gataaaacaa gaggtttttg aacagtcagg cataaccctt gaagaggaag
     8281 tggaattctg ggggttcaat ggatagggag gttctaaaag cacagagagt aggggtgctc
     8341 atgggaggaa gatcttcaga acgggatatt tctttcaaga gcggcaaagc agttattcag
     8401 ggactcaaga ggcgtggtta caacgtgacc gccattgatg ttgatcctga tcttgccatc
     8461 aaattgaagc gcaaacatat cggcgctgca tttatcgcac tccatggcag gtggggagag
     8521 gatgggtcag ttcagggttt gcttgaaatc atggggatac cttacaccgg ttcaggagtc
     8581 cttggatctg ccatggccat ggataaagtt gttatgaaaa tgatgtttga aagtatgggc
     8641 ataccatccc cggcttacac acaggcagaa gatggaggta cggttcattt tccactacct
     8701 tttgtagtga aaccggctaa tgaaggttct actattggta tatcaatagt aaaaaaacag
     8761 aaggaagtga tggcagcgat aaaaaaggcc cgtatgtttg ataaaaaagt gatggtagaa
     8821 aaatacattg aaggcgacga gataaccgtt ggtatcgtta acggagaagc ccttcctgta
     8881 gttcaggtca aacccagttc aggattctat gatttcgaag caaagtatac aaaaggaatg
     8941 acagaataca tagtgcctgc aaagatagat ataaccattg cgaaaaaagc tggagatatt
     9001 gccatggagg tatataaggt gttcgaactt gccgggtgcg tccgcatcga tatgctggta
     9061 gatgatgacc ttcccaaggt tattgatatc aatacctctc cgggaatgac agaaacctcc
     9121 cttgttccca aggcatggga aagcctcggg aggacctttg atgagcttgt tgaagcaatc
     9181 ctcatggagg catcactgaa gatatgaaaa agagccttta cacattattt cttatccccc
     9241 tgtgtgttat atctatgctt actgttattt atgtttttgc caaagacgaa ccgctctttt
     9301 ttctgagaaa tatcaaggta aatgggattc agcacttgaa agatcatgac ataatgggtc
     9361 gtgcggcgcc atttctcaga gatagcctct ttaagataga catctcagag atgaaggatg
     9421 caattcttac tcaccctttc gtgagggaag tgaggataaa gagggtctat cctttttcac
     9481 ttgtcattga tgtggatgag aaaaaggcat cagccttgtg ggcagacagt gaagggcagc
     9541 ttcatgttct tgacgacaat ggtgagccct ttagaggttt ttccagggaa gacagcaagg
     9601 gtcttattgt catcaatgcc aaagacaaga atggtgcaaa ggctgtgtac aggcaaatca
     9661 atgaatggtt ccgagagggc attataaaaa aggatgacct ctccgatgtt gcatacagcg
     9721 aagggaacat cacgatctat ggctgtggtt gtgatgatgg ggtagaaata attctgggaa
     9781 aagaagatta cacgagcaga cttaagaaag cagtggctgt tttacaggat gcaaagcaga
     9841 gaggtttttt gataaagtgt atagacgcca ggttcgaaaa aggtgctatc attaaggaaa
     9901 ggcagggtta gcatggtcac agaggatgga ctccttgtcg ggctggatat aggcactacg
     9961 aagatatgcg tagtggttgc caagattact gaaggcaagg tgaacattgt tggcattggc
    10021 tctcacccct ctaccggttt gagaaaaggc gttgtggtta atatggacag caccgtcaac
    10081 tccattaaaa aagctgttga ggaagcagaa ctcatggcag gtatcaagat tgattcctgc
    10141 cttgtgggga tagctggtgc ccatataaaa agctttaaca gtaatggcgt tgtagctatc
    10201 aaggataagg aagtaaggcc tgatgatgtt gcacgggcca tcgatgcagc aaaggcggtt
    10261 gccatacctg ccgacaggga actgatccat gtcatccctc aggaatatat cattgacgat
    10321 caggatggta ttagagatcc cataggcatt accggggtca gacttgaggt gaagacccat
    10381 atcgtgacag ggagcgtttc ttctgcgcaa aatattgtta aatgctgcaa gcttgcaggg
    10441 ttgaacgttg atgacatcat tctcggacag cttgcctcat cagaagcgac attgacgcct
    10501 gaagagaaag agatcggggt tgctcttgtt gacatcggtg gagggacgag cgatatcgct
    10561 gtcttcatca acggtgcaat aaagtttact tcagttcttc cgtacggtgg caacaatata
    10621 accaatgaca ttgccattgg tctcaggacc cctcttgagg aggcagaaaa gattaaaaag
    10681 aaatatggat gtgcctttgc aaatatgata ggggccaatg aaacgataga agttccaagc
    10741 gtaggtggta gaaagcccag aacactcatg agaaaaaccc ttgccgatat catcgagcca
    10801 agggttgagg agatatcctc tcttatctac caggagatca agaagtccgg cgctgagcgg
    10861 cttcttgctt ctggggttgt cattactgga ggatgtgcaa accttgaagg aattgctgag
    10921 ctggcagaaa acattttcaa cctccctgca aggaggggtt atcccatagg cctcgggggc
    10981 cttgttgatg ttgtaaataa tccaatatat gccaccggtg ttgggcttct tgtttatgga
    11041 tttaaaaact caagagttcg taagcatgga tttaagaagg gtacacgttt taaagatttt
    11101 ttgaatagca gaaaattact ctatagaatg agagactggt ttaaagaaat tttctaagga
    11161 ggtgtggggt gagcaacgta ttcttcatgg acgaaacgaa cggattttcg gcaaaattaa
    11221 aggttgtagg tgttggcgga ggtggatgca acgcgttgaa taacatggtt gaagccggtg
    11281 tacagggtgt tgagtttgtt gctatcaata cggacgtgaa atcgcttagt atgtgcaagg
    11341 cacctgttaa gatacagatt ggttcaaaac tcacccgggg tctcggggct ggagcagatc
    11401 ctgaggtagg gaaaaaggct gcactcgagg atgttgaaaa aataagagat catctggctg
    11461 gaacagatat ggtttttgtg acgtgcggat tgggaggcgg aacaggaaca ggagcgtcac
    11521 cggtaattgc agagatttca aaggaattgg gtgctctaac cgttgccata gcaacaaaac
    11581 cctttgcatt tgaagcgagg gctcgaatgg agcaagcaga acgtggcgtt gcacagttga
    11641 gaaattctgt agactccctc ataactatcc cgaatcagcg tcttctttcc atcggcggaa
    11701 aacatatgac agtcatggag gcatttttta aagcggacga agtgcttctc aatgcagtgc
    11761 gcagtatttc tgatctgatt gtgggctcag gctatgtggt tgtggatttt gcagatgtga
    11821 agactattat ggctgaacgg ggtatggcta taatgggcgt tggcatggct tctggagaaa
    11881 acagggcaag agatgctgca caaaaggcaa tatcaagccc ccttttagag gatatctcga
    11941 tccatggggc acggggtgtt ctgatcaatg tgaccggaaa tactgacatt actctggccg
    12001 aggtaaacga agcatccact ttgatccagg agcaagccca tgaaaatgca aaggtaatct
    12061 gggggcttgt gtttgacgac agcatggacg actcggtaag aataacagtt attgcaacag
    12121 gcttcgagga gcagatcata tctgaatcag acaatgttga atcttttcca cggtcgaagc
    12181 tttttgtgga ggatgagaat gtgccccctt tccagagaaa gaagatcgct atcgactaca
    12241 aggaaataaa agcaagaagc gacaacatcg acatagatga tgataggctc gatataccga
    12301 cattcctcag gaagcaagca gactgactca gttaaaaatg aaaagttaga agcagaagaa
    12361 agcagagccg gggattcaga gttaagagca agataataaa agtttttgaa taatgcattg
    12421 ctctttataa caccacatgt atttagctat cacttgattt ttatactctc tgcctcctcg
    12481 tactctctgt caaagctcta tgatacagcg aaagcctcgt aaggaaaacc gcctaatacg
    12541 aaggcaacgt tttgccaacg aaaagggcgt tatctctaag cggtggcacg gtaaaatccc
    12601 tgtgtgctta gttttcccca acacatacta catagggatg tcgaatcttg ccctccacat
    12661 cttgtacaca actctgaaca gccattcaga tattgtgtgc gagcgctgtt tttttgatga
    12721 aaaagaacag ttgcgttctt tcgagagtgg taaacctctc acatcgtttg acctcgtttt
    12781 tataactctt tcttttgagc tcgattttgt taatattcca aaaatcttga attgcggtag
    12841 attagctgtg ttggcgtcag agaggaagga aggtgaccct attgtggttg caggcggcat
    12901 ttgtgtgatg gcaaaccctg aaccagtaag caagtttatt gatcttttca tcctcggaga
    12961 tatagaggcg acagtgcctt tattcataaa aaaatatttg gacacaactg gtaagaatag
    13021 acatgatgta atacaggcat tgagcggttt taagtgggtc tacaatccag tcgctttgga
    13081 tgtgacatac gcttccgatg gaactattgg ggattttaag ccacatcacc atttcataac
    13141 tatcgaaaat tacaggggta aaaaccttgg ctcgtctgcg atcatcacaa gagagacaga
    13201 gtttgcaaac atgttccttg ttgaaggtac acgggggtgt ccgtcacagt gcccattctg
    13261 tcttctcggt aatatgtatc catttaacta tgatagggta gagaggtttc atccagatat
    13321 cgaagatata ggaataatcg gcggcggtgt ctcctatcat ccccgcctgg tggatattat
    13381 taaagccatg aaaaaagaag gaaaacatat ccaccttcca tccctcagag tggatgagac
    13441 cacaacacaa ctggtcgagt tgatccaaga cgaaataaaa accttaacct tcggcattga
    13501 ggcgggaagc gaacgtttgc gaaagtttat cggaaaacca ctgactgatg atgaaatcta
    13561 tgagaaactt gaaacaatcc ttgtcattaa accatttaac ctaaagcttt attttatgat
    13621 cggactttac ggtgaggata agaaggatct tgaggacatt atcagcatgg tgaagcatat
    13681 ccatcatatt atggttaaaa ctggcgcgcc caaaggtatt atgggatcaa tcacagtaca
    13741 tgtgagcccc tttgtcccca aggcatttac tccattccag tggctgccca tggaagagat
    13801 ggatgcgctg aaaggcaaga ttgcatggat caaaaagggg ttaacacgag tgggcaacac
    13861 ctactttacc cacgagtcag tgaaatacag cgttgtccaa ggtatccttg ccagaggaga
    13921 caggagggtg ggagatgtga tccttcgcct tgccagaggg gatagcttca acaaagtcat
    13981 gagggaaagc ccgctgaacc ttaacttcta tgttcataga gagagaaaca aagacgagct
    14041 ctttccgtgg gatttcataa agggcaggca gacgaaaagc gctctgtgga agcggctggg
    14101 atcactcaag tgcagcttgt agctcgtagc cgatagcaaa agaaaggact aaagtagaac
    14161 gatatattct ggattttcaa tagggatgag catggcatcg gatttgccac aaacacgctc
    14221 attcgctcca gcggctccac ttgctcgtgt tcagctttgg aacttttgcg cccgtaaaag
    14281 accgggcttc ctctcttctc aacagttttt cacgaaaacc cgatactgtc aaacataaag
    14341 tcatggggcc ctcgaataca ccataattgt gaaggtgcag cctcaagaat cgaacagagt
    14401 tgttttgagt ctcttggata tgacagctt
//
LOCUS       DAOOMC010000077        13972 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdus sp. isolate AMR_MDS_2685
            NODE_4742_length_13972_cov_6.117195, whole genome shotgun sequence.
ACCESSION   DAOOMC010000077 DAOOMC010000000
VERSION     DAOOMC010000077.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864760
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdus sp. (wastewater metagenome)
  ORGANISM  Syntrophorhabdus sp.
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae; Syntrophorhabdus.
REFERENCE   1  (bases 1 to 13972)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13972)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,729
            Genes (coding)                    :: 2,708
            CDSs (with protein)               :: 2,708
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 7 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13972
                     /organism="Syntrophorhabdus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4742_length_13972_cov_6.117195"
                     /isolate="AMR_MDS_2685"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2699756"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(7..80)
                     /locus_tag="PK125_08790"
     tRNA            complement(7..80)
                     /locus_tag="PK125_08790"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(46..48),aa:Gln,seq:ctg)
     gene            complement(326..670)
                     /gene="rplQ"
                     /locus_tag="PK125_08795"
                     /pseudo
     CDS             complement(326..670)
                     /gene="rplQ"
                     /locus_tag="PK125_08795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017042856.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
     gene            complement(680..1699)
                     /locus_tag="PK125_08800"
     CDS             complement(680..1699)
                     /locus_tag="PK125_08800"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: protein motif:HMM:NF003513.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HPB38245.1"
                     /translation="MFEKNWQELVKPTKIEVEKKEQAYIKFSTEPFERGYGITIGNSL
                     RRVLLSSIMGGAIVSVWIDGVDHEFSTIRGVKEDVTELILNLKKIVIRISDDKVKLLK
                     IDVKGPREVTAADITHDGGVEIINPDHHIATLSEEARLYMEMKAKVGRGYTSAEANYD
                     ENEPVGTIPIDAIFSPIKRVSHSVTTARVAERTDYDKLSMEIWTDGSVNPLDALSFAA
                     KIIKEQMKIFINFEDIEEISGEEEGMADKPDFNENLYRSVDELELSVRSSNCLKNADI
                     KYIGELVQKSEQEILMTKNFGRKSLNEIKEILSCMGLRLGIKLDNFPSREELDRSALK
                     RKDNI"
     gene            complement(1729..2349)
                     /gene="rpsD"
                     /locus_tag="PK125_08805"
     CDS             complement(1729..2349)
                     /gene="rpsD"
                     /locus_tag="PK125_08805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004410410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HPB38246.1"
                     /translation="MSRYVGPSCRLCRREATKLFLKGERCFTEKCAIEKRNYPPGVHV
                     DVRGKFLEYGLRLREKQKVRKIYGLSEKQFKRFFTMAERKAGVTGANFLVLLERRLDN
                     MVFRLGFATSRKEARQIVSHKHILVNGKKVNTPSFLVKEGDVISVKHKDLPNVQNALE
                     SVVRRGVPSWIELDKEEMKGTIKLLPTREDITMPIREQLIVEYYSR"
     gene            complement(2361..2750)
                     /gene="rpsK"
                     /locus_tag="PK125_08810"
     CDS             complement(2361..2750)
                     /gene="rpsK"
                     /locus_tag="PK125_08810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HPB38247.1"
                     /translation="MAKAKKTGKKKIRKSIPVGEAYIQSSFNNTIITITDPQGNVVAW
                     SSGGVVGFKGSRKSTPFAAQLAAENAAKKAMENGMKTVDVFIKGPGAGREAALRALQS
                     AGLKIHLIRDITPIPHNGCRPPKRRRV"
     gene            complement(2763..3128)
                     /gene="rpsM"
                     /locus_tag="PK125_08815"
     CDS             complement(2763..3128)
                     /gene="rpsM"
                     /locus_tag="PK125_08815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019429278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HPB38248.1"
                     /translation="MARIAGVDIPRDKRIEVALTYIYGIGKNFSKQIMTEAGIDPNKR
                     TNTLTDEEIAKIRDIIERHYKVEGDLRKEFSINIKRLMDVGCYRGLRHRRGLPVRGQR
                     TRTNSRTRKGPRKTSMKRK"
     gene            complement(3141..3254)
                     /gene="rpmJ"
                     /locus_tag="PK125_08820"
     CDS             complement(3141..3254)
                     /gene="rpmJ"
                     /locus_tag="PK125_08820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HPB38249.1"
                     /translation="MKVKPSVKKRCDKCKIVKRKGLLRVICENPKHKQRQG"
     gene            complement(3265..3483)
                     /gene="infA"
                     /locus_tag="PK125_08825"
     CDS             complement(3265..3483)
                     /gene="infA"
                     /locus_tag="PK125_08825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003130554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HPB38250.1"
                     /translation="MPKGEGIEIEGTVIEPLPNAMFRVELPNGHKVLAHVSGKMRMHY
                     IKILKGDKVVVELSPYDLSRGRIIYRVK"
     gene            complement(3491..4240)
                     /gene="map"
                     /locus_tag="PK125_08830"
     CDS             complement(3491..4240)
                     /gene="map"
                     /locus_tag="PK125_08830"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: protein motif:HMM:TIGR00500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HPB38251.1"
                     /translation="MIILKTVEEIQKIRTASKYAMEILLHLGRLVKRGIKTIDLEIEC
                     EDAIKKVGNIKPAFKGYNGFPYCLCVSVNDEVVHGMPGNRVLKDGDIVSLDFGTLYDG
                     FYGDAALTYKVGEIGKNAEKLLAVTEESLYEGIQEAKEGNRLHDISHAIQSCVEKAGF
                     SVVREFVGHGIGRSLHEEPQIPNYGERGTGIRLKKGMVFAIEPMVNMGKSDVMIKDNG
                     WTAVTKDGSLSAHFEHTIAITENGADILSRL"
     gene            complement(4289..5596)
                     /gene="secY"
                     /locus_tag="PK125_08835"
     CDS             complement(4289..5596)
                     /gene="secY"
                     /locus_tag="PK125_08835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004868351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HPB38252.1"
                     /translation="MGGFQNIGKIPELKRRIFATLALLAVYRVGVHIPTPGIDGRVLA
                     GIFEQARGTLLGFFDMFAGGGLERLSVFALGIMPYISASIILQLLTVVVPTLEKLSKE
                     GEAGRKKITQYTRYGTVIISIIQGFGIAVGLEQMRGAGGELVVYNPGWSFRLMTMITL
                     TGGTSFIMWLGEQITEKGIGNGISLIIFAGIVARMPNAIAGTMSLVNSGEMNWFVVLL
                     LIAMMFVVIAFIIFMESSQRRIPVQYAKRVVGRRMYGGQSTHLPLKINTAGVIPPIFA
                     SSILMFPATIASFINHPYMKKFSELLTPGSFLHEVLYIAFIIFFCFFYTAIVFNPDNV
                     SDNMKKYGGYIPGIRPGKKTSEYIEKILSRVTLIGAIYISFVCVLPTLLVKRFNVPFY
                     FGGTALLIVVGVALDTIQQIESHLILRHYEGLTGKSKRLKGRR"
     gene            complement(5596..6039)
                     /gene="rplO"
                     /locus_tag="PK125_08840"
     CDS             complement(5596..6039)
                     /gene="rplO"
                     /locus_tag="PK125_08840"
                     /inference="COORDINATES: protein motif:HMM:TIGR01071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HPB38253.1"
                     /translation="MNLSDLKPSEGSRKKRKRVGRGTGSGRGTTAGYGNKGQRSTSGG
                     TKPEWFEGGQMPLTRRIPKRGFKNPFRVEYALVKVGDLAVFKGKDKVSIQDFIESGFI
                     KDMKNRVKLLSDGEIDFSLKVAVHKASKKAIEKVKAQGGDVEVMI"
     gene            complement(6040..6225)
                     /gene="rpmD"
                     /locus_tag="PK125_08845"
     CDS             complement(6040..6225)
                     /gene="rpmD"
                     /locus_tag="PK125_08845"
                     /inference="COORDINATES: protein motif:HMM:TIGR01308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HPB38254.1"
                     /translation="MSHLKIKWVKSAIGRTQDQRDTIRSLGFKKLQQERIVKNTPKIR
                     GMVKKVIHLLEIVEDVK"
     gene            complement(6227..6715)
                     /gene="rpsE"
                     /locus_tag="PK125_08850"
     CDS             complement(6227..6715)
                     /gene="rpsE"
                     /locus_tag="PK125_08850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003194373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HPB38255.1"
                     /translation="MEVDGLELQDRLVYINRVAKVVKGGRRFSFSAIVVVGDGNGRVG
                     YGLGKANEVPDAIRKAVERAKKDLIQVPVAKGTIPHEIMARYGTSQVFMKPASEGSGV
                     IAGRAVRAVVEVAGITNLLTKCYGSRNYHNVVKATILGLSLLKSPDTVSRQRGKIKGE
                     EG"
     gene            complement(6747..7112)
                     /gene="rplR"
                     /locus_tag="PK125_08855"
     CDS             complement(6747..7112)
                     /gene="rplR"
                     /locus_tag="PK125_08855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016293491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HPB38256.1"
                     /translation="MDRRDKVEARDKRKKRIRKKLSGTKDKPRLCVYKSLKEIYAQII
                     NDIDGNVITGVSTLNKEIKADIKYGGNVKAAKRVGEQIGKIATGLGIAHVVFDRNGFK
                     YHGRIKALADGAREAGLKF"
     gene            complement(7124..7660)
                     /gene="rplF"
                     /locus_tag="PK125_08860"
     CDS             complement(7124..7660)
                     /gene="rplF"
                     /locus_tag="PK125_08860"
                     /inference="COORDINATES: protein motif:HMM:TIGR03654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HPB38257.1"
                     /translation="MSRIGKKPIEMPKGVKLEIKDGEVVVTGPKGTLKRSLLEGLTVQ
                     SDGNVINVQRSSEEKKIMGYHGLMRSLIANMVEGVHNGFERKLEIVGIGYRAETQGNN
                     VVFYLGYSHPITFPLPQGISAQVDKQTSLTIKGIDKELLGEIAAKMRALRKPDAYKNK
                     GVKYAEEVLKKKAGKSGK"
     gene            complement(7669..8061)
                     /gene="rpsH"
                     /locus_tag="PK125_08865"
     CDS             complement(7669..8061)
                     /gene="rpsH"
                     /locus_tag="PK125_08865"
                     /inference="COORDINATES: protein motif:HMM:NF001109.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HPB38258.1"
                     /translation="MIDPIADMLTRIRNAIMARHESVDIPYSNMKFGISKILKDEGYI
                     RNYKTFVDETRKKFLKVYISYDENSKSVITGLQRVSKPGRRVYVGAGEVKKQNRLSTV
                     ILTTSKGLMTDRNAYKNKVGGEPLLVVW"
     gene            complement(8073..8258)
                     /locus_tag="PK125_08870"
     CDS             complement(8073..8258)
                     /locus_tag="PK125_08870"
                     /inference="COORDINATES: protein motif:HMM:NF005974.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HPB38259.1"
                     /translation="MARKASMEKWSKTPKYRVRARNRCAICGRPRGYLGKFQMCRICF
                     RLNALKGSIPGVIKSSW"
     gene            complement(8269..8811)
                     /gene="rplE"
                     /locus_tag="PK125_08875"
     CDS             complement(8269..8811)
                     /gene="rplE"
                     /locus_tag="PK125_08875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003638072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HPB38260.1"
                     /translation="MKKGYGEFYEVEVKSALMRKFKYKNIMQVPKLEKIVVNVGLGEA
                     IQNIKALDAAANDIATITGQKPVITKAKKSIASFKLREGMSIGCMVTLRRERMYEFFH
                     KLVYIVLPRVRDFKGVSPKSFDGRGNYTLGLREQIIFPEIDYDKVDRVRGMNITIGTS
                     AKTDEEGFELLKLMGMPFKS"
     gene            complement(8822..9148)
                     /gene="rplX"
                     /locus_tag="PK125_08880"
     CDS             complement(8822..9148)
                     /gene="rplX"
                     /locus_tag="PK125_08880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016293496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HPB38261.1"
                     /translation="MEEKQYHVKKNDTVMVTTGKDKGKTGKVLRIVSKKDRLVVEKVN
                     MIKRHVKPSQKSKGGIMERESPIHISNVMIYCDKCSKPVKIGRKALEDGKKVRFCRRC
                     KEVIDK"
     gene            complement(9253..9621)
                     /gene="rplN"
                     /locus_tag="PK125_08885"
     CDS             complement(9253..9621)
                     /gene="rplN"
                     /locus_tag="PK125_08885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007073026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HPB38262.1"
                     /translation="MIQGRTKLEVADNSGAKKLGCIRVLGGSRKRYGTVGDIVVCSVK
                     EVIPNAKVKKGEVVKAVIVRTTKEIRRMDGSYVKFDDNSAVIINQYNEPIGTRIFGPV
                     ARELRAKKFMKIVSLAPEVV"
     gene            complement(9602..9913)
                     /gene="rpsQ"
                     /locus_tag="PK125_08890"
     CDS             complement(9602..9913)
                     /gene="rpsQ"
                     /locus_tag="PK125_08890"
                     /inference="COORDINATES: protein motif:HMM:TIGR03635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HPB38263.1"
                     /translation="MEAKVETNRRKMTGVVVKDKMDKTVVIEVEKFLKHPKYHKYLKR
                     KKRYKAHDDKSQCNVGDRVIIVESRPLSKEKRWVVKGIIEKEKLMSIREEVTGNDTGQ
                     N"
     gene            complement(9961..10167)
                     /gene="rpmC"
                     /locus_tag="PK125_08895"
     CDS             complement(9961..10167)
                     /gene="rpmC"
                     /locus_tag="PK125_08895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002509269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HPB38264.1"
                     /translation="MKAREFRDLTKEELLKKKKDFKEEIFNLRFQHSTGQLENTARMK
                     LLKRDVARIETALREKETQAGKTS"
     gene            complement(10164..10532)
                     /gene="rplP"
                     /locus_tag="PK125_08900"
     CDS             complement(10164..10532)
                     /gene="rplP"
                     /locus_tag="PK125_08900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018133712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HPB38265.1"
                     /translation="MRGAAFRGSTVSFGDYGLQALECGWVTSRQIEAARIALTRYVKR
                     TGKVWIRIFPDKPITKKPAETRMGKGKGPSEGWVAVIRPGKILYEIKGVPEDKAREAL
                     RIASFKLPIKSRFVARSEEQ"
     gene            complement(10533..11224)
                     /gene="rpsC"
                     /locus_tag="PK125_08905"
                     /pseudo
     CDS             complement(10533..11224)
                     /gene="rpsC"
                     /locus_tag="PK125_08905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529946.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
     gene            complement(11237..11569)
                     /gene="rplV"
                     /locus_tag="PK125_08910"
     CDS             complement(11237..11569)
                     /gene="rplV"
                     /locus_tag="PK125_08910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001148024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HPB38266.1"
                     /translation="MEITAKTRMIRISPRKVRIVGDLIKKRNINDAMGILTYMPQKAS
                     FILKKLLDSAIANARQKKYVDVDNLYVKNVIVDGGPVMKRFLPRAMGRATKIRKRTSH
                     ITMILDES"
     gene            complement(11574..11864)
                     /gene="rpsS"
                     /locus_tag="PK125_08915"
     CDS             complement(11574..11864)
                     /gene="rpsS"
                     /locus_tag="PK125_08915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HPB38267.1"
                     /translation="MPRSLRKGPFVEEKLLKKAEEAKGSKGAKVIKMWSRNSTIIPDF
                     VGLTFAVHNGKKFIPVFVTEEMVGHKFGEFSPTRTFHSHSGDRKAKVVKRKE"
     gene            complement(11874..12701)
                     /gene="rplB"
                     /locus_tag="PK125_08920"
     CDS             complement(11874..12701)
                     /gene="rplB"
                     /locus_tag="PK125_08920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018003275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HPB38268.1"
                     /translation="MGVKEFKPTSPGRRFMSGLTFEEITKKKPEKALLSTIKKTGGRN
                     SSGKITTRHIGGGHKRRYRIVDFKRNKFEIPGKVFGIEYDPNRSANLALIHYADGEKR
                     YIIAPHGLKVGEMIVSSKKPDTEVKEGNTMPLRYVPLGTFIHNVEMKPAKGGQLARSA
                     GSYAQLVAKEGDHGQVRLPSGEVRVVHLTCMATIGQVGNIDHENITVGKAGRPRWLGI
                     RPTVRGMAMNPVDHPLGGGEGRTKGGKHPRSPWGQLAKGKKTRRNKRTDRFIVKKRG"
     gene            complement(12701..12988)
                     /locus_tag="PK125_08925"
     CDS             complement(12701..12988)
                     /locus_tag="PK125_08925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008343846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HPB38269.1"
                     /translation="MNEYDIVVRPIITEKSSLLKDAGNQYVFEVQRDANKIEIRKAVE
                     KLFKVKVVSVHVANNEGKKKRVGRFTGKKPDWKKAIVKLNPKDKITIFEGA"
     gene            complement(12985..13608)
                     /gene="rplD"
                     /locus_tag="PK125_08930"
     CDS             complement(12985..13608)
                     /gene="rplD"
                     /locus_tag="PK125_08930"
                     /inference="COORDINATES: protein motif:HMM:TIGR03953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HPB38270.1"
                     /translation="MALTDVFDIKGDKVGEVELRDEIFTCDVKPYLMHEVVTMQLANR
                     RRGTAATKTRKEVSGGGRKPFRQKGTGRARQGSSRSPLQRGGGTIFGPHPRDYSYSVP
                     KKVRRSALRSALSVRYTGSAMKVLDKLDLEAISTKNFNGIVKTFSLTKPLFIIDKKNE
                     VVEKSARNIPFVKVLRVEGLNVYDIIRHEQLVLTLDALKRIEEVLVS"
     gene            complement(13620..13925)
                     /gene="rpsJ"
                     /locus_tag="PK125_08935"
     CDS             complement(13620..13925)
                     /gene="rpsJ"
                     /locus_tag="PK125_08935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003672692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HPB38271.1"
                     /translation="MRQRIRICLKAFDHRLLDLSVKEIVDAARKTGAQVVGPVPLPTR
                     IQKFCVLRSPHVDKKSREQFEVRTHKRLIDIVEPTQQTVDALMKLELAAGVDVEIKS"
BASE COUNT         3173 a         3737 c         2492 g         4570 t
ORIGIN      
        1 tatatttggc tgggggatag ggatttgaac cccaattcac ggagtcagag tccgttgtcc
       61 tgccgttgaa cgatccccca actttcacct gtcatgtttc tgttgtctct gttacttctt
      121 cctctgcttt ttttgttttc tttgccggtt ttttctccgc tgtcgcagtc gcctttttct
      181 cggctttttt ttctattttc tctttcttct cttttttctc tgttttttct tctgcctttt
      241 tccctgcctt tcctgtcgac ttcttttcgg ctttttttgc gggcttctta ccttccttct
      301 ctttcttctc ttccttcttc tgcgtcagtt cgataatggc catgggcgca ttatcacctt
      361 ttctgttgcc aatcttcaca acccttgtat aaccaccgtt aatagctgtg taacggtcgc
      421 cgatttcagt aaaaagcttc ttcacaatgg ctttgtctct caggacagaa aatgcaagcc
      481 ttaaagagtg gagatctttc cttttcgcga gggttatgag ccgctccgcc acctttctta
      541 attccattgc tttcgtagtt gtggtcttaa tgctctcgtg ataaaacagt gagtttgcaa
      601 gattcataaa gagcgcttct ctgtggctct tggttctgtt cagttttttt actctcttct
      661 gatgcctcat ggaaaacctc tatatattgt cttttctttt tagtgctgat ctgtccaact
      721 cctcccgtga aggaaaattg tccagtttta taccgagcct gagacccatg catgaaagta
      781 tctctttaat ctcattgagg gattttcttc caaagttctt tgtcataagt atctcttgtt
      841 ctgacttctg cacaagttcg ccaatatact tgatgtctgc gttcttcaag cagtttgaac
      901 ttctcacaga aagttcgagc tcatcaacgc tcctgtacag gttctcgtta aagtcaggct
      961 tatccgccat cccttcctct tcaccactaa tctcttcaat gtcttcaaaa ttaatgaaga
     1021 ttttcatctg ttccttgata attttcgctg caaacgaaag tgcatccagg ggattgacgc
     1081 ttccgtcggt ccagatttcc atactgagct tgtcataatc tgtccgctct gctaccctgg
     1141 cggtggtcac gctatggctc accctcttaa tgggagaaaa gattgcatca atgggaatag
     1201 tcccaacagg ctcattctca tcgtaattgg cttcggctga tgtatatccc ctgccgactt
     1261 ttgccttcat ctccatgtac agacgcgctt cttcgctcaa tgtagcaata tggtgatcag
     1321 ggttaatgat ttccacgccg ccgtcgtggg taatgtcagc tgcagttacc tcacgcgggc
     1381 ccttgacatc gatcttgaga agcttgacct tatcatcgct tatcctgata actatctttt
     1441 tgaggttaag gattaattcg gtaacatcct ctttgacacc ccgtatcgta gaaaactcat
     1501 ggtcaacgcc atcaatccag actgagacga tagcgccacc cataatagaa gacaaaagaa
     1561 cccttctcaa ggagttccca atggtaatac cataccctcg ttcaaagggt tctgtggaaa
     1621 atttaatata ggcctgttct tttttctcta cttcaatttt cgtcggcttt acaagttcct
     1681 gccaattttt ttcaaacata cgcatgtacc ctccttaagg gtaaaaggct atcgagaata
     1741 atactctact atgagctgct ctctgatagg catagtaata tcttctcttg tgggaaggag
     1801 ttttatagtg cctttcattt cttccttgtc gagctcaatc caggaaggaa cccctctccg
     1861 caccactgac tcaagggcat tttgcacatt gggaagatcc ttatgcttga ctgatataac
     1921 atcaccttct tttacaagga aagaaggtgt atttaccttt ttcccgttta ccaggatatg
     1981 cttgtggctc acgatctgcc tggcctcttt tcgtgaggtg gcaaagccga gcctgaagac
     2041 catgttatca agccttctct ccagaagcac gagaaaatta gcacctgtaa cccctgcttt
     2101 tcgctcagcc atggtaaaaa atcttttaaa ctgtttttcg cttaggccat agatctttct
     2161 caccttctgc ttttctctaa ggcgcaatcc atattctaaa aacttgcccc tcacatccac
     2221 gtgaacacct ggcggataat tccttttctc aatagcgcac ttctcggtga agcatctttc
     2281 gcctttaaga aaaagttttg ttgcctctct cctacacaat ctacatgatg gacctacata
     2341 ccttgacaat gctgccctcc ttacactctt cttcttttcg gcggcctaca cccgttatga
     2401 ggaataggcg taatatctcg tatcaagtgg atctttaacc cggcgctttg taaagccctc
     2461 agtgccgctt ctctgcccgc accaggccct ttaatgaata catcaaccgt cttcatcccg
     2521 ttttccatcg ccttttttgc ggcattctct gcggcaagct gggctgcaaa gggagtactc
     2581 tttctggagc ctttaaaccc cacaacgcct ccgcttgacc aggcaacaac attcccctgg
     2641 ggatcggtaa tcgtaatgat cgtattgttg aagcttgact gtatataagc ctccccgacg
     2701 ggaatactct ttcttatttt ctttttgcca gtttttttgg cttttgccat tgacccacct
     2761 ctttattttc tcttcattga agtctttctc ggacctttcc tcgtccttga gtttgttcgt
     2821 gttctctgtc cccgcacagg caatcccctc ctgtgccgta ggcccctgta acagccgaca
     2881 tccatgagcc gtttgatgtt gatactgaat tctttccgta aatcgccctc aaccttatag
     2941 tgtctctcaa taatatctct tatctttgca atctcttcat cagtcagggt gtttgttctc
     3001 ttattcggat cgataccggc ctcagtcata atctgctttg agaaattctt tcctataccg
     3061 tagatgtacg ttagagcaac ctcaattctc ttgtctctgg ggatatctac acctgctata
     3121 cgtgccacgc aactacctcc ttacccttgt ctctgtttat gttttgggtt ttcacagata
     3181 actctcagca gaccctttct tttcacaatt ttacatttgt cacatctttt tttaacggaa
     3241 ggcttgacct tcattgtgat cctcctattt aaccctgtaa ataattcttc cacggctcag
     3301 gtcatagggc gaaagctcta ccactacctt atctcccttc aggattttga tgtagtgcat
     3361 acgcatcttt cctgagacat gagcaagcac cttgtgaccg ttcggcaact ctaccctgaa
     3421 cattgcattt ggtaaaggtt caataacagt cccttcaatt tcaatccctt cacctttcgg
     3481 catatcccct ctataacctg cttaatatat cagcgccatt ctcggtaatg gctatggtat
     3541 gttcaaaatg tgcggacagg ctgccgtctt tcgtaaccgc tgtccatccg ttgtctttta
     3601 tcattacgtc gcttttcccc atattaacca ttggctcaat ggcaaacacc atgcctttct
     3661 tcaacctgat acctgtgcct ctttccccgt aattgggaat ctggggttcc tcatggaggc
     3721 ttcttccgat cccgtgcccc acaaattctc tcaccactga aaaaccagcc ttttccacac
     3781 aggactgaat ggcatgggaa atatcgtgca gtctgtttcc ttcttttgcc tcctgtatac
     3841 cttcatacaa tgattcctct gttactgcga ggagtttttc agcatttttc ccaatttcgc
     3901 ctactttgta ggttaatgca gcgtctccgt aaaagccgtc atacaatgta ccaaagtcta
     3961 ggctgacaat gtcaccgtct ttgagaaccc tgttaccggg catgccgtgt actacctcgt
     4021 catttaccga tacacacagg caatatggga agccattata acctttaaaa gcaggcttta
     4081 tgttcccaac cttttttata gcgtcctcac attcaatctc gaggtctatc gtctttattc
     4141 ctctttttac cagtcttccc aggtgaagca aaatctccat cgcatactta cttgcggttc
     4201 ttattttctg tatctcttct accgtcttca gtatgatcat tcaaaacaca tacaaactgc
     4261 tatcgcttaa agcttacagc tgcctttatt atcgtctccc cttcaatcgt ttactctttc
     4321 ccgtcaaccc ttcgtaatgc cttaaaatga gatgagactc tatctgctga attgtgtcaa
     4381 gggcgacacc aacaacgata agcagcgctg ttcccccaaa ataaaaggga acattgaatc
     4441 tctttacaag cagcgttgga agaacgcaga caaaagagat ataaatcgct ccgataagag
     4501 taacacggga caggatcttt tcaatatatt ctgaagtctt ctttcctggc ctgatgccgg
     4561 ggatgtaacc tccatatttt ttcatattat ctgatacatt gtccgggtta aacacaatag
     4621 ccgtgtaaaa gaaacagaaa aatatgatga atgctatata taacacttca tgaaggaagc
     4681 tgcccggtgt aagaagttca gaaaacttct tcatatacgg gtgattgata aaactcgcta
     4741 tagttgcagg aaacatgaga atagatgagg caaaaatggg agggattacg cctgctgtgt
     4801 taatcttcaa aggaagatgg gtagactgcc ccccgtacat cctcctgcca acaacccgtt
     4861 ttgcatactg tactggtatc cttctctgcg atgactccat aaaaatgatg aaggctatta
     4921 cgacaaacat cattgctatt aagagcaaca ccacaaacca gttcatttcg ccgctattca
     4981 caaggctcat agtaccggca atagcgtttg gcattcttgc cactataccg gcaaaaatga
     5041 taagggatat accgttcccg atgccttttt ctgttatctg ctcgccgagc cacataataa
     5101 aggaagtgcc acctgtgagg gttatcatgg tcataagccg gaaactccat cccggattat
     5161 agacaacaag ctcaccccct gcaccgcgca tctgctccag tccaactgcg atcccaaaac
     5221 cctggataat gctgataatg acggtaccgt aacgagtgta ctgggtaatc ttcttccttc
     5281 cagcctctcc ttcttttgag agtttttcaa gtgtgggaac tacaaccgta agaagctgga
     5341 gtatgataga agcgctgata tatggcatga tgccgagagc aaaaacagag agcctttcca
     5401 atccgccacc ggcaaacata tcaaaaaagc caaggagggt acctcgcgcc tgctcaaaaa
     5461 taccggctaa aacccttccg tctattccag gtgttggaat atggacacca accctgtaaa
     5521 ctgcaagcag agcaagagtt gcaaagatcc ggcgcttgag ctcaggaatc tttccaatat
     5581 tctggaaacc tcccattaaa tcattacctc cacgtcccca ccctgtgcct tgactttttc
     5641 aattgctttt tttgaggctt tatgaaccgc aacctttaat gaaaagtcga tctctccgtc
     5701 agatagaagc ttaaccctgt tcttcatatc tttgataaac cctgattcta tgaaatcctg
     5761 gatgcttact ttgtcttttc ccttgaaaac tgcaaggtca ccaaccttaa cgagggcata
     5821 ttccactcta aaagggtttt taaatcccct tttgggaatt ctccttgtga gaggcatctg
     5881 cccgccttca aaccattccg gttttgtgcc gccgcttgta gagcgctgtc ccttattgcc
     5941 atagcctgcc gtagttccgc gacctgagcc ggttcctctg cccactcttt ttctcttctt
     6001 ccgtgagcct tcacttggtt ttaaatcaga gagattcatt tattttacat cctccactat
     6061 ttctaagagg tgtattacct tcttcaccat acctcttatt ttcggcgtat ttttaacaat
     6121 ccgttcctgt tgaagtttct taaaaccgag gctccttatg gtatccctct ggtcctgggt
     6181 gcgtcctatc gcacttttta cccatttgat cttgaggtga ctcaccttat ccctcctcgc
     6241 cctttatctt gccacgctgc ctggatactg tatcgggcga tttcaataat gaaagaccta
     6301 gaatggtggc tttaacgaca ttgtggtagt ttcgtgatcc ataacatttt gttaacaggt
     6361 ttgtaatgcc ggcaacttca acaacagccc gtacggccct tcctgcaata acaccgctgc
     6421 cttcacttgc tggcttcata aatacctgac tcgttccgta ccttgccata atctcatggg
     6481 ggattgtacc ttttgcaaca ggcacctgaa taaggtcctt tttagcccgt tcaacagcct
     6541 tgcgtattgc gtcaggcacc tcatttgcct tacccaggcc atagccaacg cgaccgtttc
     6601 catccccgac aacaacaata gcgctgaagc tgaaccttct tccacctttt actaccttgg
     6661 cgacccggtt tatataaacc agcctgtctt gcagctcaag cccgtctacc tctatgcgtc
     6721 tcttctcaac caatcacggc ctcctcttaa aattttagtc cagcttctct cgcaccatca
     6781 gcaagggcct ttatccgtcc gtggtacttg aacccgtttc tgtcaaatac aacgtgagca
     6841 atccccaaac cagtggcaat ctttccgatc tgttccccga ctctttttgc ggccttaaca
     6901 tttccaccat acttaatatc agcctttatt tctttattca gggttgacac cccggttatc
     6961 acattgccat cgatgtcatt gataatctgg gcatagatct cttttaaact cttgtataca
     7021 cacagtctcg gcttatcctt ggttccagac aacttctttc tgattctttt ttttcttttg
     7081 tctcttgcct cgaccttatc tcgtctatcc atattttcgc tccttacttc ccacttttgc
     7141 cagccttctt ctttaagacc tcttcagcat acttaacgcc tttgttctta tatgcatcgg
     7201 gctttcgcaa ggctctcatc tttgctgcta tttctccgag tagctccttg tctatcccct
     7261 tgatggtcag cgatgtctgt ttgtccacct gagctgaaat accttgcggc agaggaaaag
     7321 taatggggtg ggaatatcca agataaaaga ctacattatt cccttgcgtt tcagccctgt
     7381 aaccaatacc gactatttca agcttccgct cgaaaccatt gtgcacacct tctaccatat
     7441 ttgcaataag ggaacgcata aggccgtgat agcccattat cttcttttct tcgcttgacc
     7501 tttgaacatt gatcacattc ccgtcagact gaacagtcag tccttcaagc aatgaccttt
     7561 tcaacgtacc tttaggtcca gtcacgacaa cttcgccatc ttttatttca agtttcacgc
     7621 cttttggcat ttcaataggc ttctttccta tcctagacat ctgttacctc accaaaccac
     7681 gaggaggggc tcccctccta ccttgttttt atatgcgttc ctgtcagtca taaggccttt
     7741 cgacgttgta agaatgaccg tgctcagcct gttttgtttt tttacctcac cggctcctac
     7801 atagaccctt cttcctggtt tgctcactct ctgcaaaccg gtgattacgc tcttgctgtt
     7861 ttcatcataa ctgatataga ccttcagaaa tttcttgcgc gtttcgtcaa caaaggtttt
     7921 atagtttctg atatatccct catctttcag aatctttgat atcccgaatt tcatatttga
     7981 atacggtatg tcaactgact catggcgggc catgatcgca tttcttattc ttgtaagcat
     8041 atctgcaata ggatctatca tcctgctccc ccttaccagc tcgatttaat aaccccgggg
     8101 atagaaccct tcagggcatt taatctgaaa catattctgc acatttgaaa tttccccaag
     8161 taacctcttg gccttccaca aatggcgcac cgattcctgg ccctcacgcg gtatttcggt
     8221 gttttactcc acttttccat actagctttt cttgccatga gtcctccgtc aactcttaaa
     8281 gggcatgccc ataagtttca gcagttcaaa accctcttca tccgtttttg cagatgtccc
     8341 tatggttatg ttcatgcctc ttaccctgtc aactttatca taatctatct cagggaaaat
     8401 aatctgttcc ctcaagccca gtgtataatt gcctctcccg tcaaaagatt taggagaaac
     8461 tcccttgaag tctctcaccc tcgggaggac tatgtagaca agcttatgga agaattcata
     8521 cattctttcc ctgcgaaggg taaccataca accgatcgac atgccttccc tgagtttgaa
     8581 ggaagcgatc gatttttttg cctttgtaat aactggcttc tgccctgtta tggtggcaat
     8641 atcatttgct gctgcatcga gcgcctttat attctgtatt gcttcaccaa gacctacgtt
     8701 gacaacgatc ttttcaagtt tgggcacttg catgatattc ttgtacttaa attttctcat
     8761 gagcgccgat ttaacttcca cctcgtagaa ctcgccgtaa cctttcttca actgaagcct
     8821 cctacttatc tataacctct ttacatcttc tgcagaatcg tactttcttg ccgtcttcga
     8881 gagcctttct acctattttc acaggctttg agcatttgtc acagtagatc attacgttcg
     8941 atatatgaat ggggctttcc cgttccatga tgccaccctt agatttctgg ctcggtttta
     9001 cgtgtctttt gatcatattt accttttcca caaccaggcg gtccttcttg ctgacaatcc
     9061 tcaaaacctt gccggttttg cccttatctt taccggtagt caccatgacc gtatcattct
     9121 tctttacgtg gtactgtttc tcttccatac ttacctcatc gtaagcaaac agctatcagc
     9181 tatcagctat cagctatatt gaagtatatt ttgctgaacg ccaatcgcca accgctataa
     9241 gccgcattcc tttcacacaa cctccggggc aagagacacg attttcatga atttctttgc
     9301 ccggagttcc cttgccacag gaccaaaaat ccttgtccca ataggttcgt tatactggtt
     9361 tataataacg gctgaattat catcaaactt cacatacgaa ccgtccatac gccgtatttc
     9421 ctttgttgtc ctgacgataa cagctttcac aacctcaccc tttttgacct tggcattggg
     9481 tatgacttct ttaacagaac aaacgacaat gtcacccaca gtgccgtatc gttttcgtga
     9541 tccaccaaga acccttatgc atccaagttt ttttgcgcca gaattgtcgg ctacctcgag
     9601 tttagttctg ccctgtatca ttgcctgtca cctcttcccg gattgacata agtttttctt
     9661 tttcaataat tcctttcaca acccatctct tctctttgct caagggtctc gactccacga
     9721 tgatcaccct gtcgccaaca ttacattgac tcttgtcatc atgagccttg taccgttttt
     9781 ttcttttcag gtatttgtga tatttggggt gtttgaggaa cttttccacc tcaataacta
     9841 cagtcttatc catcttgtct ttaacaacca cccctgtcat ctttcttctg tttgtttcaa
     9901 ccttcgcttc catggctacc tcattttaag cattcagcgt tcagcctggg ctgataacgt
     9961 ttagctggtt ttgcctgcct gtgtttcttt ttcccgtaaa gccgtctcta ttcgtgcaac
    10021 atctcgcttt aaaagtttca tccgggcagt attttccaac tgcccagttg aatgttggaa
    10081 tcgtagattg aaaatctctt ctttgaagtc cttctttttt ttcagaagtt cttctttcgt
    10141 caggtctcta aattcccgtg ctttcattgt tcctcacttc ttgcaacaaa tctcgacttt
    10201 attggcaatt taaaagaagc aattcgcaaa gcttcccttg ctttgtcctc cggcacccct
    10261 ttaatctcat acagaatctt tcctggccga attaccgcta cccaaccttc cgaaggccct
    10321 ttaccttttc ccatccttgt ctctgctggc tttttagtaa tgggcttgtc aggaaatatt
    10381 ctgatccaga cttttccggt tctctttaca taccgcgtaa gcgctatacg agcggcctct
    10441 atttgtctcg aggtaaccca accacactcc agtgcctgga gaccataatc accgaagctt
    10501 accgtgctgc ccctgaacgc agcccctctc attcttcctt tttgctgttt tctatatcgt
    10561 accctttttg gtgcaagcat tacgttgcct cctgataaat ctcaccctta aacacccaga
    10621 ctttgatacc aataacacca tattttgtac tggccgtggt aaacccatag tctatgtctg
    10681 cacgaatggt ttgcaggggt acacgtccct cacgatacca ctcggtacgt gccatctcgg
    10741 caccggcaag ccttcctgaa cacatagctt taattccttt agctccaaac tttaaggcct
    10801 gggatacgtt tcttttcatt gcccttctga aagagacacg cctttctaac tgaagagcta
    10861 tgttttctgc aacaagctgg gcatcaactt caggtctttt tatctcagtt atattaagga
    10921 tcacttcctt atccgtgatt ctctgaagtt cttttttcag attttcgacc tctgcacctt
    10981 tccttccaat tacaaggcca ggtcttgatg tatagatgtt gatcttcgct cttttgtctt
    11041 tatttgcagc ccgttcgatc tcgatcttcg atattcctgc ctggcgaagc ctttctttca
    11101 gaaattgttt tatggtgaga tcttcatgta aaaaccttgc atagtttttt gctgcaaacc
    11161 attttgaatc ccatgtcttt atgatgccaa gcctgaaacc gaaaggatta gtcttttgac
    11221 ccatttagcc tccagattac gattcatcta aaatcattgt gatgtgggaa gtccgctttc
    11281 ttatttttgt agcccttccc atggcccgag gaagaaacct cttcatcaca gggcctccat
    11341 ctacaatcac attttttaca tacaggttgt ccacatccac atattttttc tgacgcgcat
    11401 tcgcaatagc actatcgagg agtttcttca aaataaagga tgccttttga ggcatgtagg
    11461 taaggatacc cattgcatca ttaatatttc tcttctttat cagatcaccg acgatcctta
    11521 cctttcttgg agaaatccgt atcattcttg ttttcgccgt gatttccata aacctattcc
    11581 tttctcttta ccacttttgc ctttctgtct cccgaatggc tgtggaacgt tctggttggc
    11641 gagaattctc caaacttgtg gcctaccatt tcctcagtca caaagacagg tataaatttt
    11701 ttaccgttat gcacggcaaa cgtaagccca acaaaatcgg ggattatggt tgagtttctc
    11761 gaccacatct tgataacttt agcacccttg gatccttttg cttcctcagc ttttttcaag
    11821 agcttctctt caacaaacgg ccctttcctt aatgaccttg gcacgtagac ctcctaccct
    11881 ctctttttaa caataaatcg gtctgttctc ttgtttcttc tcgttttctt gcccttagca
    11941 agctggcccc acggtgaacg cgggtgctta ccaccttttg tcctgccttc cccaccgccc
    12001 agggggtggt cgactggatt catggccatt ccccttacag ttggccttat tcccaaccat
    12061 cggggtctcc cggccttgcc aacagttata ttctcatgat ctatgttgcc cacctggcca
    12121 atggtagcca tacaggttaa atggacaact ctaacctcgc ccgagggcag tctgacctgg
    12181 ccatgatcac cttcttttgc cacgagctgc gcatagcttc ctgcgcttcg tgcaagctga
    12241 ccgcccttcg caggtttcat ctccacgtta tgaatgaaag ttcccagagg tacatacctg
    12301 aggggcatgg tattgccctc ctttacctct gtatcgggtt tcttgctgga aacaatcatt
    12361 tctcccactt ttaacccatg gggtgcaatg atgtatcgtt tctctccatc tgcataatgg
    12421 ataagggcaa ggtttgcaga cctgttggga tcatattcga taccgaatac ctttcccggt
    12481 atctcaaact tgttcctttt gaagtccaca attctatacc gtctcttgtg tccgcctcct
    12541 atgtggcgtg ttgtgatttt ccccgacgaa tttcttccac cagttttttt gatcgttgat
    12601 aggagcgctt tttcaggttt tttctttgta atctcctcaa acgtgaggcc actcatgaac
    12661 ctcctgcccg gtgaggttgg tttaaactct ttaacaccca ttatgcaccc tcaaaaattg
    12721 ttatcttgtc tttgggattt agctttacaa ttgccttctt ccagtccggc tttttaccgg
    12781 taaatcgtcc gactcttttt ttcttcccct cattattcgc gacatggaca gaaacaacct
    12841 ttaccttaaa aagcttctca acggcctttc tgatctcaat cttgtttgca tccctttgga
    12901 cttcaaaaac atattggttt ccagcatctt tgagaaggga gcttttctcg gttatgatag
    12961 gtcttacaac gatatcgtac tcgttcatga tacaagcacc tcttctattc tcttcagggc
    13021 atccagcgtc agaactagct gttcatgtct gatgatgtca tatacattga gtccctcaac
    13081 ccgcaggacc ttcacgaagg gtatatttct tgccgatttc tcgaccactt cgttcttttt
    13141 atcgataatg aaaagtggct tcgtgaggct gaatgtcttc acaataccat tgaagttttt
    13201 tgtactgatc gcttcaaggt ccaatttatc gaggactttc atggcagaac ccgtataacg
    13261 cacggataaa gcagatctga gagcacttct gcgaaccttc ttcggaactg aataactata
    13321 gtcccttgga tggggtccaa aaattgttcc gccacctcgt tgcaagggag agcggcttga
    13381 accctggcgg gccctgccag tccctttttg gcgaaaaggt ttccttccgc caccactcac
    13441 ctcttttcgt gtcttcgtgg cagcggtacc ccttcttctg ttggcaagct gcatggtaac
    13501 aacctcatgc ataagatagg gttttacgtc gcaggtaaat atttcatccc taagctcgac
    13561 ttcacccacc ttatcgcctt ttatgtcaaa tacatctgta agtgccatgt ttaaactcct
    13621 caggatttta tctccacgtc gactccagca gctaactcaa gcttcatgag ggcatctact
    13681 gtctgctgtg ttggctcaac gatatcgatt aacctcttat gggttcgcac ctcaaactgt
    13741 tcccgtgatt ttttatcaac atgaggggac ctcagtacgc agaatttctg gatcctcgtt
    13801 ggcagcggca cagggcccac aacctgggca ccagtctttc tggctgcatc tacaatctct
    13861 tttaccgaca ggtccagcaa tctgtggtca aaggctttca gacatattct gattcgttgc
    13921 ctcatcttcc atcccctcta ttctatgatc tcggtgcata ccccggcacc ta
//
LOCUS       DAOOMC010000078        13912 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdus sp. isolate AMR_MDS_2685
            NODE_4780_length_13912_cov_6.114671, whole genome shotgun sequence.
ACCESSION   DAOOMC010000078 DAOOMC010000000
VERSION     DAOOMC010000078.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864760
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdus sp. (wastewater metagenome)
  ORGANISM  Syntrophorhabdus sp.
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae; Syntrophorhabdus.
REFERENCE   1  (bases 1 to 13912)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13912)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,729
            Genes (coding)                    :: 2,708
            CDSs (with protein)               :: 2,708
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 7 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13912
                     /organism="Syntrophorhabdus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4780_length_13912_cov_6.114671"
                     /isolate="AMR_MDS_2685"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2699756"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2526)
                     /locus_tag="PK125_08940"
     CDS             complement(<1..2526)
                     /locus_tag="PK125_08940"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HPB38272.1"
                     /translation="MSETKRRHRQLAETEVLQGGHTECQETATPCELDYRIIFERSND
                     AMYVVRDEGIIVDCNLKMLEMFGYTREQVIGKTVYDFSPPRQPDGRDSKEKGIEIMRA
                     SLDGNPQTFEWTHCGYGGTLFDAEVTFAKAGTDHDTAYYAIVSDVSPRRQVENAIRES
                     EEKFRLLFEKSTDPALLLDNDRFIDCNEAALKILCCPSKDRLIGLCPADLSPERQPDG
                     ALSSDKAKEVVAVASREGFNRFEWVHRAFDGTDIWVDVSLTVIPFQGRQIQYNVWRDI
                     SKRKLAEAALQVSQQKYQCIYENVAEGMFRTDAQGRLVSANAALAKMYGFARADDLIN
                     AAWNLNQVYVRPKNRKLLNRLLAEKGVARNLETEFYARDGSTRWGRMNVRVEKDEQGN
                     ILYYEGTLEDITEKKWAEDKYRDIFLNATEGIFQLTPEGRCFNANPALARIHGFDSPK
                     DLIGYAAEIPRQLHVDEETWRTYLERMNEKGYVHDQLWKMHRKDGSIAWLSINARVVR
                     NEIGHVLYHEGTVQDITEKKLAVDQIFIQRNLALRLAQMNDVSDALALTLETALKASG
                     MECGGIWLKKQTGEDLELISSIGFSHKLEKKVRLLPKGSRAWERTMSGKRIVTVPSRE
                     SMPNTYEEGYKYAVLVPILHDGHTIGSFNLYSRTEATISQQALMGLEFIAGQLGSIIG
                     RIRAQQEREHEIVTRREAEQALRAERKNLEEANIALKVLLRQREKDKEEMEQRLLLNV
                     SELVLPYIEKLKKSRLDSLHRTDLEFVETNLREILSPFLHNVRNFKLTPRQLEIVALI
                     KQGRTTKEIAETLGATKDAIDKQRFIIRKKLGANKGKINLRSLL"
     gene            complement(3303..3764)
                     /locus_tag="PK125_08945"
     CDS             complement(3303..3764)
                     /locus_tag="PK125_08945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB38273.1"
                     /translation="MKKNGVIFVTMMLVLAFVFVSHSFAQRGMRWKGSGGWGAEARYS
                     RMYDPKTVETLAGEVVSVDRITPSRGMSYGVHAMVKTEKETISVHLGPGWYIENQDVK
                     IEPKDKVEIKGSRITFQGKPALIAAEVKKGDKTLELRNESGFPVWSGWRRR"
     gene            complement(3881..4033)
                     /locus_tag="PK125_08950"
     CDS             complement(3881..4033)
                     /locus_tag="PK125_08950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB38274.1"
                     /translation="MSLQVMLMIQHMAGCFQGIKSANSPVHEKINGLMSLWFVSRIWT
                     EIAQSR"
     gene            4280..4555
                     /locus_tag="PK125_08955"
     CDS             4280..4555
                     /locus_tag="PK125_08955"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPB38275.1"
                     /translation="MRQEQALQVHPDKVQDHYEVAIAYGKQGKHREAIKAFKEAILKK
                     PDHAEAHYNLAITYLILKDRSSALREYNVLKNLDYEKAIQLFDLIHE"
     gene            complement(5131..6153)
                     /locus_tag="PK125_08960"
     CDS             complement(5131..6153)
                     /locus_tag="PK125_08960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB38276.1"
                     /translation="MSHKEPTIDEWKELFEVSRMLKELAPWKWMEDSESFGVENPETG
                     EVGYGCIMGAMGEVFGLIVYLGSEGLAVFEKIQSGNMTMEDEDIFASQKCLAVTFDDR
                     EMLDNKDLATIKSLGLKFRGRQAWPCFRSHLPGFLPWHLTGEEARFLALAIRWAMNVA
                     EKLRENPLALNPQKRGSYLVGYIVTDDGKPTWHEEWRKPVPFTAKPVSLSIDELRLAK
                     IKKNSQKSEHTWEVEFFYAPFVIHEGERPFYPYIALYSVHNNRYVVNFCLSSLTDYET
                     RFLENFLELLEKIKMFPKTMMIRKQAVYDFFKPIADVLGISLKKVKHLPAFEDARVSL
                     INNMTQ"
     gene            7199..9730
                     /gene="mgtA"
                     /locus_tag="PK125_08965"
     CDS             7199..9730
                     /gene="mgtA"
                     /locus_tag="PK125_08965"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: protein motif:HMM:TIGR01524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015444 - P-type magnesium transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HPB38277.1"
                     /translation="MDLPIKNLDAFWSLAPDTLLSRLQSSYNGLIEVEAKQRLHQFGY
                     NTLKTRKKGGLLGLFGAQFKSPIVIILLFATCLSLIVHDITDALIILAIVLISGLLGF
                     WQERGATDAVKKLLSIVQIRTTILRDGTIREIPVEEAVPGDVVILNAGDIIPGDCIVL
                     ESKDLFVDEATLTGETYPVEKEAGIIDKAVPMAKRANTLFMGAHVVSGSGKALIIHTG
                     KATEFGKISDQLRFRPPETEFEHGVRRFGYLLLEVTLILIIAIFAINVYFHRPVLEAF
                     LFSLALAVGLTPQLLPAIISVNLAHGAKRMADQKVIVKRLASIENFGSMNILCSDKTG
                     TLTEGQVQLHAVLDMNGKDSEKGLFYAYINATHETGFKNPLDEAIRAYRRFDLSQLKK
                     IDEVPYDFVRKRLTILVKKDNSNLMITKGALSNVLSVCSTAEMPEGELVDIEIVREET
                     NQRFKELSSRGFRTLGLAYKEVGPQSSLSKNDETGMIFLGLLVFFDPLKDGIAQVVNT
                     LKQSGVTLKVITGDSQFVADSVAERMNFTNRRILTGSSIRDMSNEALLQAVSAVDIFA
                     EIEPNQKERIILALRKSGNVVGYMGDGINDASALHAADVGISVNSAVDVAKEVADIVL
                     LEKDLNVLTHGVIEGRKTFANTLKYVFMATSANFGNMFSMAGASLFLSFLPLLPKQIL
                     LTNLLTDFPEMTIATDSVDKEMLAQPRRWNIRFIRNFMLVFGLLSSVFDYLTFGVLLL
                     ILHASAPEFRTGWFMESVISASLIVLVIRTRGPFLKSRPSKALLAATLLVFLVTIILP
                     YTPIGMLLGFVPISLPYVLTLLLIVVLYVISAEVAKRIFYKWARL"
     gene            9760..10368
                     /locus_tag="PK125_08970"
     CDS             9760..10368
                     /locus_tag="PK125_08970"
                     /inference="COORDINATES: protein motif:HMM:NF020893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HPB38278.1"
                     /translation="MIDPLPVIQHFTYVGIFCLLILGGLGFPFPEDGVLIMSGLLISH
                     GVIKLIPALVVIYPSLLIADFILYSFGRKYGKTVFEHKRFSKIVSAGTLSKFEEKFTR
                     WGSLVVFFGRHFVGFRAPIFLISGAVGIPWIKFLVADGLSAIITISAMLGLGYVGGNV
                     LQLFKKDITRIDHILFLVLLLLAACWFVFRYFRGRRKGGIHT"
     gene            complement(10467..11324)
                     /locus_tag="PK125_08975"
                     /pseudo
     CDS             complement(10467..11324)
                     /locus_tag="PK125_08975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611503.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase core domain-containing protein"
     gene            11468..11779
                     /locus_tag="PK125_08980"
     CDS             11468..11779
                     /locus_tag="PK125_08980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB38279.1"
                     /translation="MKAAKAKRFVHEATIDYGDQVTFTITPEPGNNIKKVVMDSRFIG
                     ILDRLTIADIKRNNKVKITFEQAPLSNATCFETTKGHAVFRDKEEHEKDSKHYERSGG
                     K"
     gene            complement(12114..13529)
                     /locus_tag="PK125_08985"
     CDS             complement(12114..13529)
                     /locus_tag="PK125_08985"
                     /inference="COORDINATES: protein motif:HMM:NF013955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IPT/TIG domain-containing protein"
                     /protein_id="HPB38280.1"
                     /translation="MKSFLMLTMIFFGLFVAALTQAQPVIVDHTCTAINQIPQQWIET
                     AKNNLRVSYGHTSHGSQLVTGIDAISAFKGAPFTFSYSSGYSAGIFLNDYVPSGDLGN
                     PDRTSWAQRTRNFLNQNGNDRNVVMWSWCGQVDGTEAEINTYLTLMNQLEQDFPAVKF
                     VYMTGHLNGSGATGRVNQRNEQIRTYARNNNKILFDFADIESYDPDGLTNYMALFADD
                     NCDYQGGHNWAAEWINANPSSELAQISGQCGSCAHSQKLNCVLKGGAFWWLMARLAGW
                     DGGIEPPEVLAQGTVGTHISIAGNGFGGKEGRVSIGNNLTGKKGNKKTKIISWNDSMI
                     ACEVTSAMSPGQYSIVVQLKEPKKAAPIVFNGAFIMMAPQIVSVDPDSGLPGEKIEVS
                     GNFFGSKKPKIYLEHPVTGKKQNCKVTDGFMNAETGASNVEFLVPRPSKRFPAGSYLL
                     KINNQVGTVTASTNFTVEPLP"
BASE COUNT         3583 a         3233 c         3025 g         4071 t
ORIGIN      
        1 gagcagggac ctcagattaa tcttgccctt attcgctccc agtttttttc gtatgatgaa
       61 tctttgttta tcaatcgcgt ctttcgtagc accgagtgtc tctgctattt cctttgtagt
      121 tctgccctgt ttgataagag cgacaatctc aagttgccga ggggtcagtt taaaattacg
      181 aacgttgtgc aggaagggag agagaatttc cctgagattg gtttccacaa attccaggtc
      241 cgtgcgatgc agactgtcaa gtctgctttt tttcagtttt tcaatgtagg ggaggacaag
      301 ttcgcttaca ttcagaagca gcctctgctc catctcctcc ttgtcctttt cccgttgcct
      361 taataacacc ttaagagcga tgtttgcctc ttccaggttc tttctctctg cccgcaaagc
      421 ctgttcagcc tcccgccgtg taactatttc atgctcccgc tcctgttgag cccgtatacg
      481 gccgatgatg ctgccgagtt gacctgcaat gaactcaagg cccataaggg cctgttggga
      541 tattgttgct tcagtcctgg aatagaggtt gaaggagccg attgtgtgtc cgtcatggag
      601 tataggcacc aggacagcat atttgtagcc ctcttcatat gtattcggca tggattcacg
      661 acttgggacg gtaacgattc gttttccgct catcgttctt tcccaggcac gtgatccttt
      721 tggcagaagg cgtacctttt tttccagctt gtgtgaaaag cctatggaag atatgagctc
      781 cagatcctct cctgtctgtt ttttcaacca gataccacca cattccatgc cagatgcctt
      841 gagagcggtt tccagggtta gggccaatgc gtctgataca tcgttcatct gtgccagtcg
      901 cagtgcaaga ttccgctgta tgaagatctg atccacagcg agttttttct cggttatatc
      961 ctgaacagtg ccttcgtggt aaagtacgtg gcctatttca ttgcgcacga ccctggcatt
     1021 gattgaaagc caggcgatgc ttccatcttt tctatgcatc ttccagagct gatcatgcac
     1081 atagcccttt tcgttcatac gttcgaggta tgtgcgccag gtttcctcat ccacgtggag
     1141 ttgcctggga atctcagctg cataccctat gaggtctttc ggagaatcaa agccgtgaat
     1201 cctggcaaga gcaggatttg cattgaaaca acggccttca ggggttagtt gaaagatacc
     1261 ctcagtagca ttgagaaaga tgtcacgata cttgtcctct gcccattttt tctctgttat
     1321 gtcctctaag gttccttcat aataaaggat gttcccctgc tcgtcctttt cgacccgcac
     1381 attcatacgg ccccatcgtg tgctcccatc ccgtgcataa aactctgttt cgaggtttct
     1441 ggccacccct ttttcagcaa ggagtctgtt aagaagtttc ctgttcttcg gcctgacata
     1501 gacctggttg aggttccagg cagcattgat gaggtcgtcc gccctggcaa agccgtacat
     1561 ttttgccagg gcagcattgg cgctcacaag acggccctgc gcatctgttc tgaacatgcc
     1621 ctctgcaacg ttttcgtaaa tacactggta tttttgctga ctcacctgca gcgcagcctc
     1681 tgcaagtttc cttttactga tgtccctcca tacattgtat tggatttgtc ttccctggaa
     1741 gggaataacc gtaagagaaa catcaaccca tatgtcggtt ccgtcaaagg cacggtgtac
     1801 ccactcgaaa cgattgaatc cttccctgct cgcaacggct accacttcct ttgccttatc
     1861 cgatgaaagt gcgccgtctg gttgtctttc aggggaaaga tcggccggac aaagaccgat
     1921 caaccgatct ttactggggc agcatagaat ttttaaggca gcctcattac agtctataaa
     1981 acgatcgtta tccagcagaa gcgctggatc agtcgatttt tcaaaaagga gtctaaattt
     2041 ttcttcactc tcgcgaatgg cattttcaac ctgtctgcga ggcgatacat cagagacgat
     2101 agcgtagtaa gcagtatcat ggtccgtccc cgctttcgca aaggtaacct ccgcatcgaa
     2161 aagggttccg ccatatccac agtgggtcca ttcaaaggtt tggggattgc catccaggga
     2221 agccctcatt atttcaatac ctttttcttt tgaatctcgc ccgtcaggtt ggcgaggggg
     2281 tgaaaaatcg tagacagtct taccgatgac ctgttctctg gtgtatccga acatctctag
     2341 catcttcaga ttacaatcaa caataatacc ttcatccctg accacataca ttgcatcgtt
     2401 tgagcgctcg aaaatgatcc tgtaatccag ttcgcagggg gtcgctgtct cttggcattc
     2461 cgtgtgtccg ccttgcaaaa cttctgtttc ggcgagctgc ctgtgtctcc tctttgtttc
     2521 gctcatgggt tgttcctctg tttccttctg gccttccgtt gcatgacatt ttgttgcgag
     2581 gaagtcagct ctatattgca cctaaatgat tcttcacaaa atgcggaaaa agcagataat
     2641 gtgaccctga ctgttttgaa aggagtgaag catgttttct ggaatatcat tttgtagatc
     2701 gggtaataac ggagggtggt ctgtgtcact ttctaccaaa gcaatcgtat tctataaaca
     2761 aagcttaaca tgattctatt cgaaatgcca acgagtcaat gtttatcttc caccgctggt
     2821 tcggcaggtg ctctgtgcca ggtgattcaa aatctacaca gtaaagtatt gaaggggtac
     2881 gcgaaaaaag ggctgaggat aaactgactc ctgatttcat gttatggaca cgtacagttt
     2941 ttcatagacc agctttttca taaaattgtt tcctataggc tacaggagtt agaaatccta
     3001 aatattctcc aatctccctg attcatcgtt cccgagcgat gccattcctg cactctctaa
     3061 tgagtcttaa tgtaatacac gtgcaaaact gttgacccgg gtaagatcaa caccactcaa
     3121 cagagaaagg gcacttgttt attgtgaaaa atattcacaa atgaagttat ctgtgttata
     3181 cttaatacag ttccccatcc tccggggcat catcaaccat gtatcccccc aatgacgtca
     3241 gtcagcatgg ttaccccggg ggccattttt tgtaatagaa ttttctactt aatcaataag
     3301 ggttaacgtc ttctccagcc actccaaacc ggaaagccac tctcgttccg gagctccagg
     3361 gttttgtccc cttttttaac ttctgcagct ataagagctg gttttccttg aaaggtaatt
     3421 cgagaacctt tgatctcaac tttatccttc ggttcaatct tgacgtcctg gttttcaatg
     3481 taccaccctg gacccagatg gacagatatt gtctcttttt cagtttttac catggcgtgc
     3541 acaccatagg acatacccct ggatggagtg atcctatcca cactgactac ttctccggca
     3601 agcgtttcca cagtcttcgg gtcatacatt ctgctgtacc gggcttctgc accccagcca
     3661 ccgcttccct tccatctcat ccctcgctgc gcaaaagagt gggaaacaaa cacaaaggcc
     3721 agaactaaca tcatagtaac gaaaattact ccattctttt tcatcttacc ctcctttttt
     3781 tattaatcca tgcttctttt ttgaaaccgg atattaatat gactttagtc atgttaatat
     3841 ttttgtcaac acctggcttt aactcgcagc aagttgtcat ctatctgctc tgtgcaatct
     3901 ccgtccaaat cctggataca aaccagagag acatcaatcc attgatcttc tcatggacag
     3961 gagaattggc tgactttatt ccctgaaagc agcctgccat gtgttggatc atgagcatta
     4021 cctgtaggct catctgtttt tgtactccac acctatcagt atttcatgga cttcgaacga
     4081 cctggaagac gtgcacgtat agcaagtatg gcaatggaac ccagtttatc cgaaatattt
     4141 ggacctgtgt caaaagagaa agaacctcaa ccgaaacaaa taactcgatc aggtaaagat
     4201 ttacattgct ggttaaaatt gatgttgaca aggaaagcca tggccattat aatttgaatc
     4261 ttccaacagg aaatctataa tgcgtcaaga acaagcctta caggtacacc ctgataaagt
     4321 ccaggaccac tatgaggtcg ctatcgccta tgggaagcag ggcaagcacc gggaggctat
     4381 caaagcattc aaggaagcca tactgaaaaa accggaccat gccgaggccc attataattt
     4441 ggcgattacc tatctcatat taaaggatag gagttctgcg ttacgtgaat ataatgtgtt
     4501 aaaaaacctt gattacgaaa aagccattca gttgttcgac ctcattcacg agtagttcat
     4561 catattcatt ctgataccaa aaatctatca atcttatatg gatcagattt tgcggtataa
     4621 ttcccatcag tttggtgcgc cctctgttga actactcttt cagtctgatt attccactat
     4681 agttttgaac aaaaactgtg gaaaacctgt gtataacatg cggatgtgaa gcatgtgcat
     4741 gtggatttta ttgagttatc ctcaactttt atataatttc atgataaagt taaatattta
     4801 attatatcac taagatagag ctttatgtga ttattcttcg atgtttgtac tgtattaatg
     4861 ctgcattttt ttgagtcgaa ctatcaacca tttgcgatgc gtgaagggat gggtgatgca
     4921 aacaccttat tttaggcagt ttccaaaggg caacagtgag ttgtggttaa cccttatttt
     4981 gcatcatctt attatttgtt tacgggaaag aacgctaccc ttaattatgt atatgtgcaa
     5041 atgattatga agatgtgtta tggcaaataa tagaataaga ctcctaagcc atcagaaggc
     5101 cagtccccga cacaatggct gcaccggctt ctactgtgtc atattgttga ttaaagagac
     5161 tctcgcatct tcgaacgccg gaagatgttt tacttttttt aaagatatcc ccaggacatc
     5221 agcaatgggt ttaaagaaat cgtagacagc ctgtttgcga atcatcatcg tctttggaaa
     5281 catctttatt ttttcgagaa gctccagaaa gttttctaag aaacgtgttt catagtcagt
     5341 gagtgatgat aggcaaaaat tgaccacgta tcggttgtta tgaaccgaat aaagcgcaat
     5401 gtatggatag aaaggccgtt ctccctcgtg gataacaaac ggggcataga aaaattcaac
     5461 ctcccaggtg tgttccgatt tttgtgagtt tttctttatt ttagccaggc ggagttcatc
     5521 gatagataat gagacgggtt ttgccgtaaa aggcacaggt ttgcgccact cttcgtgcca
     5581 ggttggtttg ccatcatcgg ttacaatata gcctaccagg taagaacctc ttttctgtgg
     5641 gttcaaggca agggggtttt ccctgagttt ttctgcaaca ttcattgccc accgaattgc
     5701 cagtgccagg aaacgggcct cctcaccagt cagatgccat ggcagaaaac ccggtaggtg
     5761 actacgaaag cagggccatg cctgccttcc tctgaacttg agaccaagcg acttaatagt
     5821 ggcaaggtct ttgttatcaa gcatctccct atcatcaaac gtgacagcaa gacatttctg
     5881 gcttgcaaag atatcttcgt cttccatggt catgtttcca gattgaattt tttcaaacac
     5941 agcaaggccc tcgcttccaa gataaacgat aaggccaaaa acttcgccca tggcacccat
     6001 aatgcaccca taacccactt cgcctgtctc agggttttcc acgccaaaag attcagagtc
     6061 ttccatccat ttccaggggg caagctcctt caacattcgg gatacctcaa aaagttcttt
     6121 ccactcatct atcgtaggct ctttgtggct catggtagct ccttttattg cagtgatagg
     6181 ataatatgta tcataaatcc tcaagaaaat gcaccattta tgtcaaagaa cataaaagta
     6241 aatagaaagc ttcttcaatg ccattcagca gaacctcatt tggagttcaa cgatttatcc
     6301 atccccaacg ttggcttact tgtgttaggg tgaacctgat ctttgattga tttgcatagc
     6361 agtgatatga aattgctatt attttgtcag cagcaccggg cacgctgccg aacccactga
     6421 taaagggcgg ttcctcacgg acaacatgaa ggacattcac ctttagccct tattgttata
     6481 caatgtcgag tgcctgcctt aatgtacgat ctgagtattc tgaaaaatct gtgagaacaa
     6541 gaatccaggc tggccaaagc attttgttca ctgtccttga aaaaacttta tcttctttaa
     6601 gtttcgtaat tttttgtgaa tataacggaa aatgttaacc gccagagcaa gacgcgccta
     6661 cccattatac cttatcgaag gtatgggatg cgttcttctc tacagtatca ctaccataat
     6721 cggctgtggc taccttgtgc ccggaattgt cgaagaaacc ccaacacagc ctcccgaatc
     6781 acgtcattgt catcaacaac gagaatagat tttgtggtcc tgtggtctct tccttgcctg
     6841 aaaacatgtg aaccgccctt accaaagacc gattagttga aatgtgggtt ttgtcagttc
     6901 agaaaacgat aaagagagaa tgccaaaaac ttaccttatg ggtttgggta atctatatag
     6961 tgcccttaat ttttttgtat agcagttact tttgaaatgc catacggttg tatatgttaa
     7021 acacatttgt ggccataccg ctcgatagac tgcaaatact accagttgca atggaaagaa
     7081 ttatgacctg gaatagggct cttttaatag aaatagaatg gtatatcttg tcagttatgc
     7141 caggagctcg catattatta ttgtcttaac aacggagtga aattataatg tttcagcgat
     7201 ggatttaccg attaaaaatt tagacgcatt ctggtcactt gcccctgata ctcttctttc
     7261 ccgacttcag tcttcctaca atggcctaat tgaggttgaa gcaaagcagc gtctccatca
     7321 atttggatat aataccctta aaaccagaaa aaagggtggt ttgctcggcc tttttggggc
     7381 gcagttcaaa agtcctattg taatcatttt actttttgct acctgcctct ccctgatcgt
     7441 ccatgacatt actgatgcat tgataatatt ggcaattgtt ctcataagtg gattgcttgg
     7501 tttttggcaa gagcggggag ctaccgatgc agtgaaaaaa ctactttcca ttgtccagat
     7561 caggacgacg atactccgtg atggtactat cagggaaata cccgtagaag aagcggttcc
     7621 cggggatgtg gtcatactca atgcaggaga tatcattcct ggcgactgta tagtgctgga
     7681 atcaaaagat ctctttgttg atgaggctac attgaccggc gaaacctatc ctgttgagaa
     7741 agaggcggga ataatcgata aggccgttcc catggccaaa cgcgcaaaca cgctcttcat
     7801 gggggcacat gttgtaagcg gaagtggaaa ggcacttatc atacatacag gaaaggcaac
     7861 tgagttcggc aagatttcag accaactcag attcagacca ccggagaccg agtttgagca
     7921 cggtgtcaga cgttttgggt acctcctttt agaggttact ctcatactca tcatcgccat
     7981 ctttgctatc aatgtatact ttcaccgacc tgtccttgaa gcgtttctct tttccttggc
     8041 ccttgcagtt gggcttactc cgcagctctt gcctgccatt atcagtgtca accttgccca
     8101 cggggcaaaa cggatggcag accagaaggt tatagtaaaa cgactggcat cgattgagaa
     8161 cttcgggagt atgaatatct tgtgttctga caagaccggt accctgactg aaggtcaggt
     8221 gcaactgcat gctgtcctgg acatgaacgg caaggacagt gaaaaaggtc ttttctatgc
     8281 ctatatcaat gccactcacg agacagggtt taaaaaccct cttgatgagg caatccgtgc
     8341 gtacaggcgg ttcgatctgt cccaactgaa aaagattgat gaagttccct atgactttgt
     8401 ccgtaagcgt ctcacaattc tcgtgaagaa ggataattcc aacctcatga tcacaaaagg
     8461 tgccctctca aacgtgctct ctgtttgctc cacagcagaa atgccagagg gagagcttgt
     8521 tgatatagag attgtccgag aagaaactaa tcaacgcttt aaggagctga gcagcagggg
     8581 tttcaggact cttggccttg catacaaaga ggtcggaccc caatcttccc tgagcaaaaa
     8641 tgatgagaca ggcatgatct tccttggcct tctggtcttc tttgaccctc taaaggatgg
     8701 catagcgcag gtagtgaaca cgctcaagca gtcgggtgtg acgctgaaag tcattaccgg
     8761 agacagccag tttgttgctg atagcgtggc tgaacgaatg aacttcacaa atcgacgtat
     8821 tctcaccggt tccagtatca gggacatgag caatgaagca ctgctccagg cggtcagtgc
     8881 cgtggatatc tttgcggaga tcgagccgaa ccagaaagag cgaatcatcc ttgccctcag
     8941 gaaatcaggg aatgtggttg gctacatggg ggatggcatt aatgatgcgt ctgctctgca
     9001 cgctgcagat gtaggtattt ccgtaaacag cgctgtagat gttgccaagg aagtggctga
     9061 catcgtgctt cttgaaaagg atctcaatgt tcttacacat ggtgtaatcg agggcaggaa
     9121 aaccttcgca aacacgctca agtatgtttt tatggctacg agtgctaatt tcgggaatat
     9181 gttcagcatg gcaggtgctt cactttttct ctctttcctt cctctccttc cgaagcagat
     9241 tcttctcacg aacctgctga ccgattttcc ggagatgacc attgccacgg atagcgtaga
     9301 taaagagatg cttgctcaac caagaaggtg gaacatcaga tttattcgaa atttcatgct
     9361 tgtttttggt ttactcagct ctgtctttga ctatctgacc tttggtgttc ttcttctcat
     9421 cctccatgca tcagcaccgg aattcaggac tggctggttt atggaatctg tcatctctgc
     9481 atctttgatt gttctggtta ttcgtaccag ggggcctttt ctgaagagca ggccgagcaa
     9541 ggctttactg gcagccactc tccttgtatt cctggtaaca attattcttc cttacacccc
     9601 aataggaatg ctcttagggt ttgtaccgat atcgctcccc tacgttttaa cactcctgtt
     9661 gatcgttgtt ctctatgtta tttccgctga agtagcgaaa cgaatcttct acaagtgggc
     9721 aaggctttga aagcgaaaca gtcaattgcg ttgtaaccca tgattgatcc tttgccagtc
     9781 atccaacact ttacgtacgt tggcattttc tgtctcttaa tccttggcgg ccttggattt
     9841 ccctttcctg aagatggggt gctcatcatg agtgggttac tcatctccca tggagtcatc
     9901 aaacttattc ctgcgctggt cgttatctac ccatccctgc tcatcgctga tttcatcctc
     9961 tattctttcg gcagaaagta cggcaaaacg gtttttgaac acaaaaggtt ttccaaaatc
    10021 gtttccgctg gtaccctctc gaagtttgaa gagaaattta cgcgatgggg ttctctcgtt
    10081 gttttctttg ggaggcactt tgttggattc agggcaccca tatttcttat ttctggggct
    10141 gttggtatac cgtggataaa atttttggtt gctgacggtc tctctgcgat cattacaatc
    10201 tcagccatgc ttggactcgg atatgttggg ggtaatgttc tccagctatt taaaaaagac
    10261 attaccagaa tagatcatat cctttttctc gtattattac tgttagcggc ttgctggttt
    10321 gtttttagat acttcagagg caggagaaag ggtggtattc atacataacc gttatggctt
    10381 cgtatagggt cttaccctat tcattaaccc atgacaattg aaatgttgaa gaacatctgg
    10441 tatgcacccc acaatgagac aactagttaa gtcctgcctg ctgcaagaaa cttctcttcg
    10501 aacacacttg ggggaagata ccccaaggat gagtggtgcc gatcctcatt gtacatctcg
    10561 ttgataaagc agtcaatttt ctgccgtgct tcgtgtatat tctggtactc acagaggtag
    10621 acctcttcgt atttcaatgt ttttataaag ctctcagctt tgacattgtc ataggggttg
    10681 ccttttcgtg cctgctgatc caaatggtgg ataagtccag gttgaatgta cctggccaca
    10741 agggacttct tgagtgcaga aagtggtaaa gaggtgtcca gatgttcacg taattcccag
    10801 ccgatacact tctgagagaa agcatcgagg atgtctgcca ggtagatgaa ctcatcacgg
    10861 agcctgatat aggtaatatc tgctacccag agctggttga tggaggatat ggtcatgttc
    10921 gggatgaggt tgggataggc ctgggtacta gggaatgttg ttttgaccca tcctctcttt
    10981 ctcaggcaca gaagattatc ctctctcatg ggtcatctga ggaaacatca tgatcctctc
    11041 ttgtggttgc ttccctgcat gacttttttt aaaaaatcat tctccatggt gagctgaccg
    11101 atcttccgtt ccaactgtgc gatacgggca tcctctttgt atatgtgtcc gtatcccaga
    11161 aaggcattgt cttcgtattt ccgatattca cggtaccacc gagtgaccac accatgatcg
    11221 atctcatgtt cccgtactat ctgagccagc gttttagagc cttccagttc tttcacgatc
    11281 tggagcttga actcccgggg aaagtttctc cgtgttctga ccatatgccc atacctcctt
    11341 catatagtat ggacttaact tactgtctca acaaggggcg cagttcaaga cagtaacaat
    11401 aggcaatggc gctgtgtctg caaaaggacc cctctcggtg gcaaatggga ttaatggtga
    11461 aacaccgatg aaggccgcga aagccaaacg atttgtacac gaagccacca tcgattatgg
    11521 tgaccaggtt acgttcacga tcacacctga gcctgggaat aacatcaaaa aagttgttat
    11581 ggacagccgg ttcattggaa ttttggatcg attgaccatt gccgatatca agcgtaacaa
    11641 taaggtgaaa ataacatttg agcaagcccc tctatcaaat gccacatgtt ttgagacaac
    11701 gaaggggcac gctgttttcc gggataagga ggaacatgaa aaagattcca aacattacga
    11761 acgatctggc ggaaaatgat gacgatgaaa gactcattgc cgaaccaaca cctgcaggcc
    11821 aatacgcctc tgtaaaaaat tagagcgtga cggctcgtgc acaattgaga caatctctct
    11881 caggttacta tactgattaa atatcttcac aacctggtag gttaacacca ggttgcattc
    11941 agtaatcaag catgacatat tgttgttgaa ggcaaagcca ggcaacctta atgccttaaa
    12001 ttgccgcatc ctgttcttac agggttcaca acgagttgat tggcaagtag gatgaaacaa
    12061 atgatattaa ttgacaatca ttgattacac agtggtattt ttgtcattgt tagctacggc
    12121 aacggttcta ccgtgaagtt tgtactcgct gtaaccgttc ccacctgatt attgatcttc
    12181 agcagatatg aaccggcagg gaaacgtttt gagggccttg gaacaagaaa ctctacattg
    12241 cttgcgccgg tttctgcatt catgaaacca tcggtgactt tgcagttctg cttttttccg
    12301 gtgacggggt gctcaaggta aattttcggc ttctttgacc caaaaaaatt acctgagacc
    12361 tcgatctttt ctcccggcaa gcctgaatcg ggatcaactg atacaatctg cggcgccatc
    12421 ataatgaatg cgccattgaa aacgatcggt gcagctttct tcggttcctt cagttgtacc
    12481 actatgctgt attgcccggg tgacattgca ctggtaacct cacacgcgat catggaatcg
    12541 ttccagctga tgattttggt ctttttattt cctttcttcc ctgtcaggtt attccctatg
    12601 gataccctgc cttccttccc accgaagcca ttaccggcta ttgagatatg ggtgcccacg
    12661 gtaccctgag caaggacttc cggcggctct atgcccccat cccatccggc aagcctggcc
    12721 atgagccacc agaatgcccc gcccttcaga acacagttta gcttctggga gtgggcacag
    12781 ctgccgcact ggccagagat ctgggccaac tccgaagaag ggtttgcatt gatccattcc
    12841 gcagcccagt tatgaccgcc ctggtaatcg cagttgtcat cggcaaaaag cgccatgtaa
    12901 ttcgtcaacc cgtcagggtc gtagctttca atgtccgcga agtcgaacag aattttattg
    12961 ttgtttcttg cgtacgtgcg gatctgctca ttccgttgat ttaccctacc agttgcgccg
    13021 gaaccgttca gatgccctgt catgtataca aacttcacag caggaaaatc ctgttccagc
    13081 tggttcataa gagtcaggta cgtgttgatc tctgcctctg ttccgtccac ctggccgcac
    13141 catgaccaca tgaccacatt ccggtcattg ccattctggt tgagaaagtt tcttgtcctc
    13201 tgagcccagg atgtacgatc tgggtttccc aaatctccgg aaggcacata atcgttcaag
    13261 aagattcccg cagagtagcc gcttgaataa gaaaatgtga acggagcacc tttgaaggca
    13321 cttatagcgt ctattcctgt caccagctga ctgccatggg atgtatgacc atagctgaca
    13381 cgaaggttgt tttttgctgt ctcaatccat tgctggggta tctggttaat agccgtgcag
    13441 gtgtgatcaa cgattactgg ctgggcctgc gtcagagccg caacaaacaa tccaaaaaaa
    13501 atcattgtca gcatgagaaa cgatttcatt gatgtcctcc tcttactata ggcattataa
    13561 attttctggt ttcgtatttt cattctacca taagtgtgct atttgccaat gccaaacatc
    13621 atcattacat ctttattagg aagcctcgtt ataagaaaac aggtgttcgg taatatctaa
    13681 ttatgagtca atgatccctt tttcataagc tccagggtaa gatttaatgt gaggcaattc
    13741 gcgacccctc atgaatcagg gttttattgc ttaacgataa aaaagtggga caaaataaca
    13801 tttaattata actcacagga aatatgcagt tggacaactt tgataaaaat gagtgaaatc
    13861 attaatgcct gggacgggaa tcgaacccgt acagagcaga gccccgaggg at
//
LOCUS       DAOOMC010000079        13773 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdus sp. isolate AMR_MDS_2685
            NODE_4849_length_13773_cov_6.473320, whole genome shotgun sequence.
ACCESSION   DAOOMC010000079 DAOOMC010000000
VERSION     DAOOMC010000079.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864760
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdus sp. (wastewater metagenome)
  ORGANISM  Syntrophorhabdus sp.
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae; Syntrophorhabdus.
REFERENCE   1  (bases 1 to 13773)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13773)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,729
            Genes (coding)                    :: 2,708
            CDSs (with protein)               :: 2,708
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 7 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13773
                     /organism="Syntrophorhabdus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4849_length_13773_cov_6.473320"
                     /isolate="AMR_MDS_2685"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2699756"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(84..1721)
                     /locus_tag="PK125_08990"
     CDS             complement(84..1721)
                     /locus_tag="PK125_08990"
                     /inference="COORDINATES: protein motif:HMM:NF014398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0042279 - nitrite reductase (cytochrome,
                     ammonia-forming) activity [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-forming cytochrome c nitrite reductase
                     subunit c552"
                     /protein_id="HPB38281.1"
                     /translation="MRTKTLLYTTIFVAVAFFVISNELYAQKRKSAPTGVDVSECYSC
                     HDTIKDLRKMGKHVKVECTNCHSNLEKHLINPGSETRPNTNTSWEACGQCHKEQYKSS
                     FEVAYHRPARDDKSQLTNRSPNPFWDKLMMGHGFTKEHALTRSHPWSLIDHLLVDRAY
                     GGRFQPKKGWEYTSEKPHSKIWDILYDKFPDVKEHKAFLPQTAAGANPTCFQCKTQDQ
                     ILDWAYMGDPGNGAKWSRMSKPNELAKSVKHSLNCYTCHDPHAARPRIVRDALIEALN
                     KPAGDTLWHTDPKRTIFKVIDMGMRGYPRKIAILEKYDTKLQCGQCHMEYNCNPGTDT
                     KTGQPVGFADRRTNHAPFKDSLGLYDHYVNQIHFLDWRHPLTGGLLWKAQHPDSEAYY
                     NSKHAKANVSCNDCHSPKVRDKKTRKTYTSHFVVTPRFQLKETCLKCHSGWTEEQARY
                     TIDSVKAYTKGRMRKAEFWISNLIDKIVEAKVANVDTVILKEAQNQHLKAHILWEFWT
                     AENSDGFHNPGLARESLTGAIDEAQKGIAILNKAMGK"
     gene            2002..2436
                     /locus_tag="PK125_08995"
                     /pseudo
     CDS             2002..2436
                     /locus_tag="PK125_08995"
                     /inference="COORDINATES: protein motif:HMM:NF013756.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
     gene            2539..2694
                     /locus_tag="PK125_09000"
                     /pseudo
     CDS             2539..2694
                     /locus_tag="PK125_09000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009325829.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="rubredoxin"
     gene            2781..3668
                     /locus_tag="PK125_09005"
     CDS             2781..3668
                     /locus_tag="PK125_09005"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: protein motif:HMM:NF010568.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HPB38282.1"
                     /translation="MEKALIKTNFVDIGTPKRGKVRDIYDLGEKLLIVASDRLSAFDV
                     VLPTGIPDKGKVLTKLSVFWFKQVEDIVKNHILEVDVDRYPEILKKHSDSLRDRSMLV
                     KKARIIPVECVVRGYLSGSGWSDYKKTGTICGIELPEGLVESSKLEHPIFTPTTKAEE
                     GHDMNISFEDLTAMIGERLAATLRDLSIKIYEKGCEIAERKGILIADTKFEFGIVDNE
                     IVIVDEILTPDSSRFWSKKLYKAGGAQDSYDKQIVRDYLLTLDWNKTYPGPELPESIA
                     AKARARYIEILEILTSQGL"
     gene            complement(3710..5017)
                     /locus_tag="PK125_09010"
     CDS             complement(3710..5017)
                     /locus_tag="PK125_09010"
                     /inference="COORDINATES: protein motif:HMM:NF018513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HPB38283.1"
                     /translation="MEGPVVGVYGIIVMFAILFLTKIPAAFVMALVGFLGVAYVTSFQ
                     AALGMIGADMWNIFSSYGLTVIPMFILVGEFAHYGGYNNSLYSATYKWFGHYKGGLSI
                     TTIMACAIFSAISGSNTATAATMSTVAIPEMKKYNYHPQLSAGSVAAGATLGVLIPPS
                     IVLVVYGLYTGQSIGKLFFGNVIPSAILTVLIASTVFYICWRHPDWGPKGPRSGWIER
                     IKALPDLVDIFILFGIIMYALFTGVVTATEAAAASCAIALVLCLARRKLSWSGFLKSV
                     SDTLWISCLVFMIVAGAVIFGRFLAVTRLPFEAAAMIGSLALPSWVLFWIIIACYIIG
                     GCVMDALAFLLVSLPIFYPIVMQMGYDPIWFGQVICIVTTMGSIMPPIGICCYVVAGM
                     AKDIPLGTVFRGSFYYIPSYILSIIVLMLFPYATVMILSDLVR"
     gene            complement(5020..5505)
                     /locus_tag="PK125_09015"
     CDS             complement(5020..5505)
                     /locus_tag="PK125_09015"
                     /inference="COORDINATES: protein motif:HMM:NF016200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HPB38284.1"
                     /translation="MKYFEQAIIFLNKIFLIVGGIAVLALMSVATANVVLRIFHIPFR
                     GAYELVSFIGAIVIAFALGFTQQRKGHIVVDILTERYPKGLSKAFDIVNHLVTMIFFT
                     IISWQIYRVGMQIWNSGELSETLKIIYHPFIFAVSLGFITLSLTALVQLITDIQGGGE
                     K"
     gene            complement(5610..6647)
                     /locus_tag="PK125_09020"
     CDS             complement(5610..6647)
                     /locus_tag="PK125_09020"
                     /inference="COORDINATES: protein motif:HMM:NF015445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HPB38285.1"
                     /translation="MGIKRALVLSVVVTLFFGFGLILPQTSHSQATITLKYANFPPAT
                     TFPCIQMERWAKEVEKRTAGKVKIQTFPGGTLLPAKNIFDGVIAGTADIGNFAMSYQP
                     GRFPISEAIDLPVGFTTAKAASMALYDLIEKYNPKEFEKVKMITLFTCPPADFMTKTA
                     VRSLKDLKGMELRTSGTGTEVLKRIGATPVGMPQSEAPEAIQKGIVKGNVSSMEILKD
                     FNFAAYLPFATEANLFVVSFAVVMNKDKWNSLPADVKKVIDDMRREQAEWTGKYVDDH
                     VQESLQWSKEKYNHQLIKLSKTDMDDIAKLTRPMIDEYIKKVNGLGLPGDQIVKDVYS
                     LRAKYEKQYTK"
     gene            complement(6779..7726)
                     /gene="pdxA"
                     /locus_tag="PK125_09025"
     CDS             complement(6779..7726)
                     /gene="pdxA"
                     /locus_tag="PK125_09025"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: protein motif:HMM:TIGR00557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HPB38286.1"
                     /translation="MTMGDPAGIGGEIILKALHQLSRKSIPLIIGDYSVINDHLRTIK
                     TMSKRPHFKGFMEGGKGDVEFIDIGCIRGLRFGAIDGLYGQASYAYLIEALKLVFLGD
                     VSAIVTCPISKASFHKGGISFPGHTEILAHFGGVTDYIMMMANKIMRVSLVTIHIPLK
                     HVPEEITIERVFKCIALTHHSLRTYWGIGNPSIKVCGLNPHAGEHGIMGNEENAISEA
                     INRAKSIGMEVGGPYPADTLFHSPDCHAYIAMYHDQGLIPVKTVDFRGTVNITLGLPF
                     IRTSPGHGTGFDIAGKGIADPSGLVEAYRTAEEMCIKTS"
     gene            complement(7738..8670)
                     /locus_tag="PK125_09030"
     CDS             complement(7738..8670)
                     /locus_tag="PK125_09030"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF025010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HPB38287.1"
                     /translation="MKIITCIVIVIISFIFPVLCAGEVMDRIIAIVNDDIITLKEAEK
                     HVQVEKEGRFVSINEYMTNLRLKEKIDLLIDDVLIRQEAKRMKIDVEEKEVEGIIENI
                     KKQYLIDDTELMQKLKDDNISLDDFKIGLKNNVLRSRLLNRVISPEVNVTEQDLRQYY
                     DKHKEEFIDEEYRLQQIFVSGQREDAQKRIIEAYKLLQEGKPFEVVVKDFSDDKKSSA
                     TDGDIGFVKKIDLIPQLRDVAGMLSPGVYSSIITTPYGFHILKLVEKKRGETMTFEMA
                     KEAINEKIIQEESEKRYNDYIVKLRKGSYIEVKI"
     gene            complement(8674..9657)
                     /locus_tag="PK125_09035"
     CDS             complement(8674..9657)
                     /locus_tag="PK125_09035"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF024545.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HPB38288.1"
                     /translation="MKKYLVCLLMALLVVSCAKKDNGKILATIDDDKITLQEFNKELD
                     KIPMNMKMLVASESGKKNYLDRLVVKKLLLKEASKAKIESEKEFQDRVNDIREQLLIE
                     ALLKKKISADTQMSEDDLKKYYEEHKEDFKKDREINTRHILLKTEEEAKQVQAKLQKG
                     EDFVELAKKYSIDPNVRQSGGEIGFQPKGSLIPEFENAAYKLNKVGQVSGIVKTQFGY
                     HIIRLEGVKPPSYVPLDEVKEFIKQKNSQEKQKEVLEKYIEELKKNAKITINEALLKD
                     EKSEQPVKAESPDKASSPQKVGTPQKQEAPSKQPEAEPSKSSVKEEPQPKK"
     gene            complement(9778..12942)
                     /gene="mfd"
                     /locus_tag="PK125_09040"
     CDS             complement(9778..12942)
                     /gene="mfd"
                     /locus_tag="PK125_09040"
                     /inference="COORDINATES: protein motif:HMM:TIGR00580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HPB38289.1"
                     /translation="MRELKKTGTLYITGQIGSFLSFLLSQAGRKALIFYETDDEALIL
                     REEIEFFSRKSAYIFPIYSDRVFEKEDEVKRTAFLHHLAMDTDFIGLYPCSAVGHNII
                     SREMILDNAKAIHFGDTLFQEDLVRYLDDQGYEVAPLVREEGQYAKRGSIIDVFSSSS
                     DRPVRIEFFGDEIYSIRFFDPLSQRSVKEIESYNLTSIKSLINSNTTIIDYIGEGMIL
                     VHKGFKHMEVALKETVNVPQFMEALQRKFFSNLSIDISGVQEDGGDMIEALSNDDLRH
                     LFELRKAEIFNTLSEKMRTEWWDSPYLYLFASSPRQAERLHEIFQSYQITLPILKEIN
                     CPQKQKEWGIVVGPLRRGFRTHDIIFLTEEDIIGPKKRVMKKKWDGFDDFLNSFKDLA
                     IGEWVVHIEHGIGIYQGITELKIEDCRKDFLVIEYQDRDKLYVPIDDLHLVQKFIGGE
                     KYKPKIDKLGTQYWINTKRRVKKNVEDIAKELIELYAEREIAEGHQYPPEDELFKEME
                     SRFEYEETEGQIQAIEDVLRDLKSSKPMDRVICGDVGFGKTEVALRASFKVVMDNKQV
                     AILVPTTILAQQHYKNLHERFKDYPVNIDMLSRFRSKEEQKHIIEDVRRGKIDIVVGT
                     HRILQKDLDFHDLGLLIVDEEHRFGVKHKEKLKNIKKNVDVLSLSATPIPRTLHMALS
                     GIKDLSIINTPPLDRLAIKTYVVKFNDELIKKGILNELQRGGQVFFVHNYIHNIGVVQ
                     DHLMKLLDGARIAVAHGRMEGKQLEKIMLDFIDKKYDILLSTNIVESGLDISNVNTIF
                     INNAHRMGLADLYQLRGRVGRSTKQAYAYLLVPKEEVLTKDSTLRLKIIEELTELGSG
                     FHIANYDLEIRGAGNLLGKEQSGNVNLIGFELYCNMLEDAVKQMKNEAVPVEGEIVTE
                     MNLPIDAYIPDLYIEDSTQKLLTYKRLSKVREESDLKDIEAELKDRFGDIPRPLSNLL
                     KIISLKCFLSRVKIKKIEYADKHIILHVTDKTPVDMKRLLLLVKQKGKIKLLPDNRII
                     IYADTKAAELLEFTKNVLMELVTV"
     gene            complement(13086..13670)
                     /locus_tag="PK125_09045"
     CDS             complement(13086..13670)
                     /locus_tag="PK125_09045"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HPB38290.1"
                     /translation="MALSTIFIMMLLCFAFVSQKPTRILYKEHLKKEEIVNRVTSLVR
                     VENESLNDNELRTIAKIVYDESLKKKLDYRLVLAIMKVESNFRYSAISRKGARGLLQV
                     KPSLAKYIAHDVGVHWKDDTTLDEPEKNIKIGVHVFSKLVEDFDSINMALHAYHVGPT
                     KLKEIVAEKKIPQKRYLNLVLNEYDRNKSILPPP"
BASE COUNT         3674 a         2976 c         2874 g         4249 t
ORIGIN      
        1 tttgacctgc gcaactgggg cctggatttg gagcgaggtg caatgttttt attattcttt
       61 tgcttacagc tgggttatca tctctatttc cccattgcct tattgaggat agcaattcct
      121 ttctgggctt cgtcgatggc acctgttaag gactcacggg cgagacccgg gttatggaaa
      181 ccgtcagaat tttccgctgt ccagaactcc cagagaatgt gggctttcag atgctggttt
      241 tgagcttcct taagaattac agtatcaacg tttgcaacct ttgcctcaac gatcttgtcg
      301 ataaggttcg aaatccagaa ttcagctttc cgcatccggc cttttgtgta agcctttacc
      361 gagtctatag tgtatcgtgc ctgttcctcg gtccaaccag aatggcattt taagcaggtc
      421 tctttcagct gaaaccttgg agtcacaaca aagtgtgatg tgtaggtctt tcttgtcttt
      481 ttatctctaa ccttcggaga gtgacagtca ttacagctaa catttgcctt cgcatgtttt
      541 gaattgtagt aggcttcaga atcgggatgc tgggctttcc agagaagccc tcctgtaaga
      601 ggatgtctcc agtcaagaaa atgaatctgg ttcacgtaat gatcataaag tccaagagaa
      661 tctttgaatg gagcatgatt tgttcgtctg tcagcaaaac caacaggctg gccggtcttt
      721 gtatctgttc ctggattaca attatactcc atgtgacact gaccacattg aagctttgta
      781 tcatatttct ccaggatagc aatttttcgt ggataaccac gcatccccat atctatgacc
      841 ttaaaaatag tcctttttgg gtcggtgtgc cataatgtat caccagcagg cttgttcagg
      901 gcctcgatta gcgcatctct tacaattctt ggccttgctg catgggggtc gtggcaggta
      961 taacagttca ggctgtgctt aacagatttt gcaagttcat tgggcttaga catccgtgac
     1021 cattttgccc cattgccagg gtcgcccata taggcccagt caagaatttg gtcctgggtt
     1081 ttacattgaa aacaggtagg atttgcccct gctgcggtct gaggaaggaa ggctttgtgc
     1141 tcttttacat caggaaactt gtcataaagg atatcccaga tttttgagtg tggcttttca
     1201 gatgtgtatt cccacccttt tttcggttgg aatctgcctc catacgccct gtctaccagg
     1261 agatgatcga taagactcca cggatggctt cttgtaaggg catgttcttt ggtgaagccg
     1321 tgccccatca ttaatttgtc ccagaaggga ttgggagacc tgtttgtgag ctgggattta
     1381 tcgtccctcg ccggtcggtg gtaagctact tcgaaagatg acttgtactg ctccttgtgg
     1441 cactgtccgc atgcctccca agaggtattt gtgttgggcc ttgtttctga gcctggattg
     1501 ataaggtgtt tttcaaggtt gctatggcag tttgtacact ctactttcac atgtttcccc
     1561 attttgcgta aatctttgat cgtatcatgg caactgtagc attcgctgac atcaacgcca
     1621 gtaggtgcgc ttttcctttt ttgcgcatat aattcgttac ttataacaaa aaatgctacc
     1681 gcaacaaaga ttgtagtgta cagcaaggtt tttgtgcgca tggagtcccc ctttgtttca
     1741 atctgctgta aaaaaagttt acggttattg gtattggtca tgcaagagaa attttcaata
     1801 aagtgttccg agcaattatt ttggtagaaa gatttgggag aaaaagttac aattacagtt
     1861 tctctgacta cggactcaat atatggattt aattaaggtg tttgtcagga tatagtatga
     1921 atagacctat cttccgaaac tcaagaacgg atgaatgtag gccggtgggc tctattcttt
     1981 aaaaattaag gaggatattt tatgaacatc aaagctgtcc agaaaatctg ttatgggatg
     2041 tatgtcatta gttctaaaaa agatgataaa ataaacggcc agattgccaa tacacttttt
     2101 cagataacct ctgacccgcc aacagtagct gtgagtatca acaaacaaaa cctaacccat
     2161 gaatatatca ccacaagcaa aacgtttaca gcatcaatta tttcaaaaga ggctcccatg
     2221 aattttattg ggaactgggg ttttaaatca ggcagggata tagataaatt caaagatgcc
     2281 aatttcaggg ctgggaaggt tacaggtgtt cctatagtcc ttgactatgc cactggttat
     2341 cttgaatgtg aggtactaag cagtaccgat gtgggaactc acacgatttt tatcggaaag
     2401 gtagtcgatt gtgaggtttt aagtgatgca gaaccaatga cctattcata ttatcgtcag
     2461 ataaagggtg gaaaatcgcc aaagactgcc ccgacatacg ttaaagagga gagtaccgcg
     2521 gttggagcaa cgggtgacaa atacaaatgt accgtctgtg gttatattta cgaccctcag
     2581 aacggtgacc ctgattccgg gatcaaggca gggacaaaat ttgaagatct gcctgatacc
     2641 tgggtatgcc cggtatgtgg tgcaccaaaa agcgaatttg agaaggaagg atagtatttg
     2701 ccatatacgc tgcgttgggt gctggataaa gttgaaaaga accacttaat gcgaaaagac
     2761 cgaaaaaaag gaaggctggc atggaaaaag cactcataaa aactaatttt gttgacattg
     2821 gcacacccaa aagaggaaaa gtgcgagata tttatgatct cggtgaaaaa ctccttatcg
     2881 ttgcaagtga cagattatcc gcttttgatg ttgtccttcc cactggaatt cctgacaaag
     2941 gaaaagtgct gaccaaactc tccgtattct ggtttaagca agtagaggat atagttaaaa
     3001 accacatact cgaagtagat gtggacagat atcccgagat tcttaagaag cacagcgatt
     3061 ccctgagaga ccgcagtatg ctggtaaaaa aagcacgtat tataccagtt gaatgcgttg
     3121 ttcggggata cctttccggc tcaggctgga gtgactacaa aaagacaggt acaatctgtg
     3181 gaatagaatt gcctgaaggt ctggttgagt caagcaaatt ggaacaccct atttttacgc
     3241 caacgacaaa agcagaagaa gggcatgaca tgaacatctc tttcgaagac ttaacagcaa
     3301 tgattgggga aagacttgca gcaaccctcc gggaccttag catcaaaata tatgaaaagg
     3361 ggtgtgaaat tgcggaaagg aaaggcattt taattgccga cacgaaattt gagtttggta
     3421 tagtcgataa tgaaattgtt attgtggacg aaattttaac ccctgattct tcaagattct
     3481 ggtcaaagaa gctttacaag gcgggtggtg cccaggacag ctatgacaaa cagatcgttc
     3541 gggattatct tctgacgctt gactggaata aaacatatcc tggtcctgaa ctgcctgaat
     3601 caattgcagc aaaggcgcgg gcgcgataca tcgaaatact ggagattttg accagccagg
     3661 gactgtagaa tattagctaa ttatgcgtca catgtcacgg attactttgc taacgtacaa
     3721 gatctgacag gatcataacc gtggcatagg ggaaaagcat gagcacaatg atagacagaa
     3781 tatatgacgg tatgtagtaa aagcttcccc taaacacggt acccagaggt atgtcctttg
     3841 ccatacctgc aacaacatag cagcagatac cgattggtgg cattattgag cccatggttg
     3901 ttacaataca gatgacctgg ccaaaccata tggggtcata gcccatctgc atcacaatcg
     3961 ggtaaaatat gggcagagat acgagaagga aagccaaagc atccataaca catccaccaa
     4021 taatgtaaca ggcaataatg atccagaaaa gaacccaact cgggagggca agggaaccta
     4081 tcattgccgc tgcttcaaag ggaagtcttg tcacggcaag aaaccgtcca aatatgactg
     4141 cacctgcaac aatcatgaaa acgagacagg aaatccacag agtgtctgaa acagatttaa
     4201 gaaacccaga ccatgagagt tttctcctgg cgaggcagag aacgagggcg atggcacagc
     4261 tggctgctgc agcctcagtg gctgttacaa caccggtaaa gagggcatac attataatac
     4321 cgaaaaggat aaaaatatct acaaggtctg ggagcgcttt gatccgttca atccacccac
     4381 tcctcggtcc tttgggaccc cagtcgggat ggcgccagca gatatagaat actgtcgatg
     4441 caataagaac ggtaaggatt gcacttggta taacatttcc aaaaaaaagt ttgcctatag
     4501 attgccctgt ataaagccca tagacaacga gtacgatact tggagggata agcactccaa
     4561 gggtagctcc ggcagcaact gatccagcgc tcagttgagg gtggtaatta tattttttca
     4621 tctcagggat ggcaacagtg ctcatagttg cagccgttgc agtgttggaa ccacttatag
     4681 ctgaaaaaat agcgcatgcc atgatcgtcg ttattgatag cccacctttg taatgcccga
     4741 accacttata cgtagcacta tacaggctgt tgttataacc tccgtaatgg gcgaactcac
     4801 ctacaaggat aaacataggg atcactgtta atccataact agaaaagatg ttccacatat
     4861 ctgcgccgat cattccgagg gcggcttgaa acgatgtaac gtaagcaacc ccgagaaaac
     4921 cgaccagagc catgacaaat gctgctggga tcttcgtaag aaagagtatt gcgaacatga
     4981 cgatgatgcc atatacacca acaaccggac cttccatctc tatttctccc ctcccccttg
     5041 aatatcggtt ataagctgaa caagagctgt tagtgataat gttatgaacc ctaaagatac
     5101 ggcaaaaata aatggatggt atataatctt caaggtttcg gaaagctctc cggagttcca
     5161 aatctgcata cctaccctgt atatttgcca ggaaattatg gtaaaaaaga tcatggtgac
     5221 aaggtggttt acgatatcga atgccttgct taatcctttt ggatatcttt ctgtaaggat
     5281 atctacaaca atatggcctt ttcgttgctg ggtgaatcca agagcaaatg cgataacaat
     5341 tgccccgatg aatgatacaa gctcataggc gccgcgaaat gggatgtgaa agattcttag
     5401 tactacattt gctgttgcaa ccgacataag cgcgagcact gcaataccgc ccactatgag
     5461 aaatatcttg tttaaaaata taatggcttg ctcgaagtac ttcatgaaaa gcctccgtga
     5521 agctgatatg ttggtaaatg attggtcttt catgcaaaga ggtatctatg gctatgcata
     5581 aagacacctc tttgcatcat gtcctatact tactttgtat attgtttttc gtatttcgct
     5641 cttaaagagt atacatcctt gacgatctgg tcgccaggaa gacccaggcc gtttaccttc
     5701 ttgatgtatt catcgatcat cggcctggtg agctttgcga tatcgtccat atctgtttta
     5761 gacaatttta tgagctgatg gttgtatttt tcctttgacc actgaagaga ttcttgtacg
     5821 tgatcatcca cgtatttgcc tgtccattct gcctgttcac gcctcatgtc gtcgatcacc
     5881 ttcttcacgt ctgcaggcag ggaattccac ttatccttat tcatgacaac tgcaaaggaa
     5941 accacaaaga gattcgcctc tgttgcaaaa ggcaggtatg ctgcaaagtt aaagtctttg
     6001 aggatttcca tggaagacac gtttcctttc acaatgcctt tctgtattgc ttctggtgcc
     6061 tctgattgag gcattcctac aggcgttgcg ccgatacgct ttagtacttc agtacctgtc
     6121 ccggatgtac gaagctccat tcctttcagg tcttttaagg accttaccgc tgttttcgtc
     6181 ataaagtcgg ccggcgggca ggtaaagagc gtaatcattt tcactttttc aaattctttc
     6241 ggattgtatt tttctatcag gtcgtacagg gccatacttg ctgctttcgc ggtggtaaat
     6301 cctacaggaa gatcgatggc ttctgaaata ggaaacctac caggctggta gctcatggca
     6361 aagttgccaa tatccgcagt acctgctatt accccatcga agatattttt tgcaggaaga
     6421 agagtgcctc cgggaaaggt ctgaatcttt actttcccgg ctgtcctttt ttccacctct
     6481 ttagcccagc gctccatctg aatgcacggg aacgtagtgg caggtggaaa attcgcatat
     6541 tttagcgtga ttgttgcttg gctatgtgag gtttgcggca ggataagacc aaagccaaaa
     6601 aaaagtgtaa ctacaacaga tagtaccaaa gctctcttaa tacccataac ataccccctt
     6661 aatgtttttt aatcttgaac ccccctctct tttgccgatg ccttggtgac gcttaaccca
     6721 ttgtatacag tatccaaaaa taggtatgat acacttgtac tttatgaacg cctttttgtc
     6781 aagaagtttt tatgcacatt tcttcagcag tacggtaggc ttcaacgagc cctgacggat
     6841 cggcaatccc ttttcctgca atatcaaatc ctgtgccgtg gcctggagat gtcctgataa
     6901 aagggagtcc aagggtgata ttaacagttc ctctgaagtc tactgttttc acgggtatga
     6961 ggccctggtc atgatacatg gcaatatatg catggcagtc cggggagtga aacaatgtat
     7021 cagctgggta tggccctccg acttccatac ctatcgattt agctctgttg attgcttctg
     7081 atatggcatt ttcctcgttt cccattatac cgtgttcacc tgcatggggg tttaagccac
     7141 acactttgat cgatggatta ccaatacccc aataggttct gagagagtga tgggtaaggg
     7201 caatacactt aaaaacacgc tctatagtaa tttcttccgg gacatgctta agtggaatgt
     7261 ggatcgttac gagagagact ctcatgattt tattagccat catcataata tagtctgtca
     7321 ctccgccaaa atgtgccaga atttctgtat gccctggaaa acttatccct cccttgtgaa
     7381 aagaggcttt gctaatgggg catgttacaa tggcagaaac atctccaaga aaaacaagtt
     7441 taagagcctc aatgaggtaa gcataggaag cctgtccgta taacccatca atagctccaa
     7501 agcgaaggcc cctgatacaa ccaatgtcaa taaattctac atcccctttt cctccttcca
     7561 tgaacccttt gaagtggggt cttttgctca tggtcttgat ggttcggaga tggtcattaa
     7621 taacagagta atcgccaata atgagaggga tgctttttcg ggacagttgg tgaagcgctt
     7681 tcagaataat ttccccaccg atgccggcag ggtcacccat ggtaattgcg atcctgttca
     7741 gatcttgacc tcgatatatg atccttttct cagtttaacg atatagtcat tgtaacgctt
     7801 ttcggattct tcctgaataa tcttttcatt gatggcctct ttcgccattt caaaggtcat
     7861 cgtttcaccc cttttctttt ccacaagctt caaaatgtga aaaccatagg gtgttgtgat
     7921 tatcgaactg tatacaccag gcgaaagcat accagcaaca tcgcgtaatt ggggaatcaa
     7981 gtcaattttt ttaacaaaac ctatatcccc atccgttgca gaacttttct tatcatctga
     8041 gaaatccttg acaacaacct cgaaaggttt tccttcctgg agaagtttgt atgcttctat
     8101 gatacgtttc tgagcatctt ctcgctgtcc agatacaaag atttgctgta atctgtattc
     8161 ttcatctata aactcttctt tatgcttgtc gtagtattgt ctgaggtcct gttcagttac
     8221 atttacctcc ggagaaataa cgcgattcag cagcctgctt ctgagcacat tgttcttaag
     8281 acctatttta aagtcatcga gactgatgtt atcatccttt aatttttgca ttaattctgt
     8341 gtcgtctata aggtattgtt tttttatatt ttcaataatc ccctcaactt ccttttcctc
     8401 aacgtcgatc ttcattcttt tcgcctcttg ccgtatgagg acatcatcga tgagtagatc
     8461 gatcttctct ttaagtctta aattcgtcat gtattcatta attgagacaa acctgccttc
     8521 tttttcaacc tggacatgtt tttcagcttc cttaagggtg atgatgtcat cattaacgat
     8581 tgcaatgatt ctgtccatta cctcccctgc acagagaaca ggaaaaataa atgaaatgat
     8641 aacaatgaca atacaggtta tgattttcat atcttatttt ttgggctgtg gttcttcctt
     8701 tacgcttgat ttcgacggtt cagcttctgg ctgttttgaa ggcgcctcct gtttctgtgg
     8761 tgttcctact ttttgaggtg acgaggcttt gtctggagat tcagccttaa caggctgctc
     8821 agacttctca tctttcaaga gcgcttcatt gattgttatc tttgcgtttt tcttgagttc
     8881 ctcgatatac ttctcgagca cttctttttg tttttcctgt gagttcttct gttttataaa
     8941 ctcctttacc tcgtccagcg gcacgtagct aggtggttta acgccttcta accttataat
     9001 atgataaccg aattgtgtct ttacgatacc actgacctgg cccaccttgt ttaatttata
     9061 ggcagcattt tcaaattcag ggattagcga accttttggc tgaaaaccaa tctctcctcc
     9121 tgactgtctc acattaggat cgatagaata tttttttgcg agttcaacaa agtcctctcc
     9181 tttttgcagt ttcgcctgaa cctgctttgc ttcttcttca gtctttagga ggatatgtcg
     9241 tgtattgatc tcacggtctt tcttgaaatc ttccttgtgc tcttcataat actttttgag
     9301 gtcatcctca gacatctgtg tatccgcaga aatcttcttc ttcaggaggg cctctataag
     9361 cagttgttcc ctgatatcgt taacacggtc ctgaaactct ttttcacttt ctatttttgc
     9421 cttcgatgct tccttgagaa gaagtttttt gacaaccagc cgatcaagat agttcttttt
     9481 cccgctctct gaagctacaa gcattttcat gttcatgggt attttgtcga gttctttatt
     9541 aaactcctgc aaggttatct tatcatcgtc tatggtggcc agtatcttgc cattatcttt
     9601 cttcgcacat gaaactacaa gcaatgccat aagcaggcat actaaatact ttttcattca
     9661 atcacctcat tgttttttga tatgtattca gatatgagtt gtacgtgaca aaatgagaaa
     9721 cattgataag aaaaaatact aacttctcat aactgaaagt ttatctcagg agttatatca
     9781 cacggtcaca agttccatca atacattttt tgtgaattca agaagttcgg ctgcctttgt
     9841 atcggcgtat ataatgattc tgttatcagg gagaagctta atttttcctt tttgctttac
     9901 cagtagcaga agccgtttca tatcaacagg tgttttgtcg gttacatgga ggataatgtg
     9961 cttgtcagcg tattcaatct ttttaatctt taccctggat agaaagcatt tcagtgagat
    10021 gattttgagg aggttcgaaa gtggccgtgg aatgtctcca aaacgatcct tcagttcggc
    10081 ttctatatcc ttaagatccg attcttcccg cacctttgaa agcctcttgt aagtgagaag
    10141 tttttgagtg gaatcttcaa tgtataaatc agggatatag gcatctatgg gcaaattcat
    10201 ctctgtcaca atctcgccct ctactggaac tgcttcattc ttcatctgtt tgactgcatc
    10261 ctccagcatg ttgcagtaaa gttcgaatcc gatcaagttt acatttcctg actgttcttt
    10321 ccccagaagg tttcctgcac cccgtatttc taagtcgtaa tttgctatgt ggaagcctga
    10381 cccaagttct gtgagctctt caatgatctt caggcgtaat gttgaatctt ttgtgagaac
    10441 ctcttctttt gggacaagga ggtaggcata tgcctgcttt gtgcttcgtc caaccctccc
    10501 acggagttgg tagagatcgg ctaaccccat gcgatgggcg ttgttgataa agatcgtgtt
    10561 gacgtttgat atatccagcc ctgattcaac aatattggtg gagaggagga tgtcatattt
    10621 tttgtcaatg aaatcaagca ttattttctc cagttgtttg ccttccattc ttccatgggc
    10681 tacggctatt cgcgcaccat ctagtagttt cattaaatgg tcttgtacga caccaatatt
    10741 atgaatgtaa ttatgaacaa aaaacacctg tccgcccctt tggagttcat taaggattcc
    10801 ctttttgata agttcatcgt tgaattttac gacataggtt tttattgcca gcctgtcaag
    10861 gggtggagtg ttaataatac tcaggtcctt aattccggat aaagccatgt gaagtgttcg
    10921 cggaatcggt gttgcgctca ggctgaggac atcgacattt ttctttatat tcttgagttt
    10981 ttccttgtgt ttcacaccga aacgatgctc ttcatcgaca ataagaaggc ctagatcgtg
    11041 gaaatccagg tctttttgga gaatcctgtg ggtccctaca acaatatcaa tcttaccgcg
    11101 ccttacatcc tctatgatgt gtttttgttc ctctttagat ctgaaacgac tcaacatatc
    11161 tatgtttacc ggatagtctt tgaaacgctc gtggaggttt ttgtaatgct gttgggccaa
    11221 gatggttgtg ggaacaagga tggcaacctg cttgttatcc atcacaacct taaaagatgc
    11281 acgtaacgct acttcagtct taccgaagcc tacatcccca caaataaccc tatccatagg
    11341 ctttgagctc ttcaaatccc ttaacacatc ttcaatcgct tggatctgcc cctctgtttc
    11401 ttcatattca aaacgtgatt ccatctcttt aaaaagctca tcttcaggag ggtactggtg
    11461 accctctgct atttcacgtt cagcgtaaag ctcaatcaat tccttcgcaa tatcttcaac
    11521 atttttcttt acccttcttt tcgtatttat ccaatactgt gttccgagtt tatcaatttt
    11581 gggcttgtat ttttctccgc cgataaactt ctgaacaaga tggagatcat caataggcac
    11641 atatagcttg tccctgtcct gatactcaat gacgaggaaa tctttcctac aatcttcaat
    11701 cttcagctct gtgattccct ggtatatgcc gatcccatgt tcgatatgga caacccactc
    11761 tcctatagcg agatctttga aagaattaag gaaatcatca aatccatccc acttcttttt
    11821 catgaccctc ttcttgggtc caatgatatc ctcttcagtg aggaaaataa tatcatgtgt
    11881 cctgaagcct cttctgagag gtcccacaac gattccccat tctttctgtt tctgtggaca
    11941 gttaatctcc ttgagaatag gcagagtgat ttgataactc tgaaaaattt catggagccg
    12001 ttctgcctgg cgcggactac tggcgaagag gtacagatag gggctatccc accattctgt
    12061 tctcattttt tcggataatg tattgaatat ttcagccttc ctcagttcaa agaggtgtcg
    12121 aagatcatca tttgacaacg cttctatcat atcgcctccg tcttcttgta cacctgagat
    12181 atcaatactg aggtttgaga aaaattttct ttgcaatgcc tccataaatt gaggcacgtt
    12241 gactgtttcc ttgagcgcca cctccatatg cttaaagcct ttgtggacaa ggatcatgcc
    12301 ctcgcctatg taatctatga ttgttgtatt gctgttaatt aaagatttta tagaggtaag
    12361 gttataagac tctatttctt tgacagatcg ttgagaaagg gggtcgaaaa agcggatgga
    12421 atatatttca tcgccgaaaa actctattct tactggtcga tctgaagatg atgagaaaac
    12481 atctatgata cttccccttt tggcatactg accttcctcc ctcacaaggg gtgccacttc
    12541 atatccctgg tcatcaagat agcgaactag gtcttcttga aacagtgtat ctccaaaatg
    12601 aatggctttg gcattatcca gaatcatttc ccggcttata atgttatgcc ctactgcgct
    12661 acacgggtaa aggccgataa aatctgtatc cattgcaaga tgatgaagga atgctgtacg
    12721 cttgacctcg tcctctttct cgaaaacccg atctgaatat atgggaaaaa tgtatgcgct
    12781 ttttcgggaa aagaattcta tttcttcacg caaaataagt gcttcatcat cagtctcata
    12841 aaagataagt gcttttctcc cggcttgaga gaggaggaat gatagaaacg atcctatttg
    12901 tccggtgatg tatagcgtgc ctgttttttt gagctcgcgc atggataggg tattctacta
    12961 tataccgggt tagatggtaa aatctgcatt catagtttaa aacggggctt tcagagggcc
    13021 tcgacaggac aatctgcgct tttttttcct tctcagctta gtgataacag tttccagtct
    13081 tgttgctaag gtggcggaag gatcgactta ttcctgtcat attcgttgag gacaaggttc
    13141 aggtagcgtt tttgcggtat tttcttctcc gcaacaattt cctttagctt tgtcgggcca
    13201 acatggtagg catggagtgc catgttgatg ctgtcgaaat cttctacaag tttagagaaa
    13261 acgtgtaccc cgattttaat atttttttcc ggctcatcga gcgttgtgtc atccttccaa
    13321 tggacaccca catcgtgcgc tatatacttg gcaagagatg gtttcacctg caggaggccc
    13381 cttgcccctt tcctcgaaat tgcactatac cgaaaattgc tctcaacttt catgattgca
    13441 agaaccagcc ggtaatcaag ttttttcttg agtgattcgt cgtacactat ttttgcaata
    13501 gtcctcaact cattatcatt caggctttca ttttcaaccc ttaccagact ggttacccgg
    13561 tttactattt cttccttttt gaggtgttct ttatacaaga tacgggtcgg tttttgtgag
    13621 acaaaagcaa agcacaacag catcatgatg aagattgttg acaatgccaa ttttttctga
    13681 agtgacataa gtcaagaata tacaacaaac aaaattgaga tgtcaacctt gcctccttga
    13741 gatggtaacc ttgatgtctt ctccttcctc tac
//
LOCUS       DAOOMC010000080        13169 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdus sp. isolate AMR_MDS_2685
            NODE_5202_length_13169_cov_5.950740, whole genome shotgun sequence.
ACCESSION   DAOOMC010000080 DAOOMC010000000
VERSION     DAOOMC010000080.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864760
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdus sp. (wastewater metagenome)
  ORGANISM  Syntrophorhabdus sp.
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae; Syntrophorhabdus.
REFERENCE   1  (bases 1 to 13169)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13169)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,729
            Genes (coding)                    :: 2,708
            CDSs (with protein)               :: 2,708
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 7 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13169
                     /organism="Syntrophorhabdus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5202_length_13169_cov_5.950740"
                     /isolate="AMR_MDS_2685"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2699756"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(498..1100)
                     /locus_tag="PK125_09050"
     CDS             complement(498..1100)
                     /locus_tag="PK125_09050"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HPB38291.1"
                     /translation="MAVKHGLISFDIPYDGDVLKNLCLYVTELTKWNRKINLVGLKSE
                     DVIVVDLLYDAFYLYAYVKKAVSVLDMGSGAGILAIPLKILNKEMKVFSIDRTLKKIQ
                     FQRHIQRTLKFNTFFPIHGRIESIEALNVQCLIAKAFGSTLSILTKGGRHVIKGGHAF
                     LLRGMKREVECVEGFVLQNLIPYTLPDSVKARNLFVYQRY"
     gene            1158..2477
                     /gene="lptA"
                     /locus_tag="PK125_09055"
     CDS             1158..2477
                     /gene="lptA"
                     /locus_tag="PK125_09055"
                     /inference="COORDINATES: protein motif:HMM:TIGR03302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0001530 - lipopolysaccharide binding
                     [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide transport periplasmic protein
                     LptA"
                     /protein_id="HPB38292.1"
                     /translation="MSRLIAIIASCLVLFSCTSKPVKKSENPGSLYVEGVNLMKAKKY
                     DKAINKFSDVRDNFPFDPMALISTVKLGDAYFERKTYDLALMTYEDFFKAHPEDENIP
                     YVLLRIGECYEKLSLSLDRDQAYTLKAIERYTYLMNRYPSSSYAANAEESIKRLNQKL
                     ADRELYIGEFYYRTNQYNAAITRLEYLLKKYPHTVGFDKALYFIAMSYKALGDPVKSN
                     EYIEKLQKEYPHSKLNKSSNSRGVRQTLKSSKKEFPPIDQSSTKRREIDLRPQIIKAS
                     TKGETVRVTPDKQQAGQTDKDKSGLGFIDRTKPIDIVSDTMEGFDKEKMVVFKGSVVA
                     RQDDLYIFSDVVETFLNEESNEISKAYAKGSVKIVKQDRTATAQEAFFDNIKREIILK
                     GDVVVFQGQDKITGNVITYYINEDKVVVEGDPEKRARAILTPKPKNQ"
     gene            complement(2704..3126)
                     /locus_tag="PK125_09060"
     CDS             complement(2704..3126)
                     /locus_tag="PK125_09060"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HPB38293.1"
                     /translation="MEESKKAIYFKKVQEEPLARLLNIRLKDVDDGYALCEMEFTESL
                     ENIYGAAHGGAIFSLIDEAFEISSNSHDNVAVALNMNVTYMRAPKKNSTLTAESREIN
                     RTRRTASYYITVQEGPNLIAVCQALVYVKSEEIPFLKQ"
     gene            complement(3128..3454)
                     /gene="trxA"
                     /locus_tag="PK125_09065"
     CDS             complement(3128..3454)
                     /gene="trxA"
                     /locus_tag="PK125_09065"
                     /inference="COORDINATES: protein motif:HMM:NF012314.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HPB38294.1"
                     /translation="MSSAAAVTDSDFTKEVLESTIPVVVDFWATWCGPCQVMGPVVDA
                     IAGEYAGKVKILKLNVDENQATPAKYGVRGIPTLILFNKGEVVDRLVGAQPKSNVENL
                     LKKVIA"
     gene            complement(3832..6027)
                     /locus_tag="PK125_09070"
     CDS             complement(3832..6027)
                     /locus_tag="PK125_09070"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HPB38295.1"
                     /translation="MPEKIELPITGMSCAACAARIERELNKLEDIGEARVNFPLKKAV
                     IVPKKELELKQIISLIRDIGYDVDIEADVTIRARKEEAELKRDFVWSACFSAIVMVFS
                     MWMVLPNMQYQNFILLILTLPVQFYFGLRFHKATLLNLKHLTADMNTLISVGTSAAFF
                     YSVFVTFFPHTVMKVGLEPMTYFDSSATIITLILLGRFFESKAKTRTYTAIKMLYELS
                     PKECVLLQEGKETKVPTDTIEVGNLILIRPGEKVPIDGEVIDGNTYVDESMITGESLP
                     VHKNVGDEVIGGTINGKGSVTVRVTRIGKDTVLAKVIKLVEEAQFTKAPVQRLADKVA
                     GVFVPIVILISIAAFIVWYFFGPDPKLTNGLLSFVSVLIIACPCALGLATPTAIMVST
                     GVGAKRGILIKSAEALELTNRARYVLFDKTGTLTEGVIVLAESIPLGRHSEEELLFVS
                     YNLERQSEHPFSEALRKKAEELKIEISNVDEFQAIVGKGIKGAINGITYYVGNLTLYE
                     DVGRVLDVSVKELYRKEELSGCSPVLVWSKESIIGLLCFSDRVREESKDVVSDLKSMD
                     IHVAMITGDSENGAKTISEKVGIDQFFYRVLPDQKAAFVEDFKKKGVTIMVGDGINDA
                     PSLAASDIGVAMGKGTDIAIESADVVLMKGQLSKLVSLIKLSKKTLWVIKENLFWAFI
                     YNILGIPIAFGALYPFFGIRLDPMFGALAMMLSSISVVSNSLRLKFFKE"
     gene            complement(6312..6794)
                     /locus_tag="PK125_09075"
     CDS             complement(6312..6794)
                     /locus_tag="PK125_09075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB38296.1"
                     /translation="MMWSWKLIYEDETFNLFCDIDNVAGSEEFDNGIFPSADCYRPLP
                     EKIVLWVSIGIKDKSVLKDYVERRKQSGLSFEGYNDFSHTLGVVEFDAENRLYRVIPA
                     VDLDTRDQQLGTSSLLDGKKASLLKGIKSDWSKIESPRTSKAIKSLYHFFYTPASLSA
                     "
     gene            7693..8268
                     /locus_tag="PK125_09080"
     CDS             7693..8268
                     /locus_tag="PK125_09080"
                     /inference="COORDINATES: protein motif:HMM:NF014796.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-Mn family superoxide dismutase"
                     /protein_id="HPB38297.1"
                     /translation="MYTAKDYSKLIGMEGLSDTLLKNHFTLYQGYVTNTNKIIELLAQ
                     MLKENKENTPEWAELKRRMGWEFNGMRLHEYYFENMGGKASLNKDGKLSKKMAEDFGS
                     YETWEKAFRAVGSMRGIGWAVLYQDNTNGRLINFWINEHDAGHPTGCSPLLVLDVFEH
                     AFMIDYGLGRTNYINAFFKNIDWAVVEGRLK"
     gene            8377..9786
                     /locus_tag="PK125_09085"
     CDS             8377..9786
                     /locus_tag="PK125_09085"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HPB38298.1"
                     /translation="MNKKKQSVFFSTVYWVFLVAALSSHPSVSFAEESSGNKTFEAIL
                     SNGLKVILLENHKAPVVTVQVWYRVGSRNETWGKTGLSHLLEHMMFRGSKRFSAEQFV
                     RMIEENGGKSNAFTSHDYTAYFVNICAERVAIPLELEADRMQNATFDEKKFATEKKVV
                     IEERRLRTENNPQAYLYEQMAATAYQVHPYHWPVIGWMEDLEGLTVSDALDYYHRYYV
                     PANAFLIVVGDFSRQDLLPKIEKAFGCLPSTQAPQQVVSREPIQKGERRIVIKREGLV
                     PALMIGYHVPNLHNTDGYVLEVIEGILSSGKSSRFYNRLVREKQIALRAYADFSLTSF
                     DPGLFFISAEVLVEKDLTTLEKAIDEEIIRLQTEPVAMEELNKVKNQLEAGFIHSQDS
                     VFNQAMVLARNEIAATWRCIDDYIPAIHRVTPNDILRVARQYMIPENRTVGYFIPLPP
                     QNIESNNSKNLIVEEEYTR"
     gene            9783..11129
                     /locus_tag="PK125_09090"
     CDS             9783..11129
                     /locus_tag="PK125_09090"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HPB38299.1"
                     /translation="MNNVKPRRLSPYWWPLNFIFFLILFPSHSFSLPPTERMSLQNGL
                     HLIVSEAHSLPFVTFQLIIDGGSRFDPPNKEGLSYLTAKGLLHGTTKHTSKQMNELLD
                     FMGANLHTDSSKDYITVGFKVLKKDLFQGFHLFMDVITLPVFPKDEFHSEIQRTLGII
                     KSVEEDPKELSEREFFKTLFAGTPYGHPVIGTEESILKLTRNDLAAFHSSYVRPNRAI
                     LVITGDISLNEIKMDLMSRLEAWPAGNVLPTSHKQPSAQNTKILTLERAISQANITLG
                     NLAPQRNNPDFFPMIVTNYILGGGFSSRLLTEIRAKRGLAYSIRSTFHFKKRVGSFLV
                     SLQTKNDSAQEAISIVLEEIKKLQKEPVTDIELEGARKYLTGSFPLQLDTQAKLAGFL
                     AQVEFYGLGEDYAHNYVSCIMAVTKEDVQRVAKKYLNPYNYTLVIVGNLKEGRFRK"
     gene            11274..12647
                     /locus_tag="PK125_09095"
     CDS             11274..12647
                     /locus_tag="PK125_09095"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: protein motif:HMM:TIGR02037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Do family serine endopeptidase"
                     /protein_id="HPB38300.1"
                     /translation="MFITVPGFSPGIERVKSPIELSTAIIQVAKKNIPAVVHIGVTGQ
                     QEVATPSLPFNDDPFFRRFFGIPQGPRKFKRETRGLGTGMIIDSQGHVLTNYHVVGNA
                     DKIDVLLSDGSSFHAKLVGSDPKTDLAVIKISAKEPLPFVTFGDSDKVEVGEWVVAIG
                     HPRGLDQTVTQGIISAKHRRGITDPSSYQDFLQTDAAINPGNSGGPLLNLRGDVVGVN
                     TIIVSGSGGFEGIGLSIPSNISRHVARLLIAHGKVERGWIGISAQDLTPESAKNLATE
                     TRKGILIGDVVKGGPADKAGIKKNDVVIAFQGKEVSNAATLRNEISLSPIGKDIRMVI
                     LRGGRKQEATVRIGDSKDAVKALSTSVRERLGVDVRSLTQKEADKFRLNINEGVLVTF
                     VDQKGPLGRVGFEVGDIILEINGQSVGGIEGFSQMIASLRPNQRITLLAFDHSSGNSG
                     YVNVTSR"
     gene            12855..>13169
                     /locus_tag="PK125_09100"
     CDS             12855..>13169
                     /locus_tag="PK125_09100"
                     /inference="COORDINATES: protein motif:HMM:NF013916.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB38301.1"
                     /translation="MKIHANDVIVPLDVPKAFQETYTRNYLEITRESGHLMLFAGDQK
                     VEHLNKDFFGEGIPADDNDPEHLFRIASEAKIGVFATQLGIIARYGMSYPAVPYLVKV
                     NSK"
BASE COUNT         3905 a         2865 c         2676 g         3723 t
ORIGIN      
        1 gattttcctt tcctgctgaa caggtggaac taaagtacaa ggaactctgg taggtggaac
       61 agatcatccg gaacatgaag tccatccgga taccaggtct atcctctatc aaatgaaacc
      121 ataaaaggtc ataccttcta cagttttttt gtcctcacag cctgtcatga ttttgaatgg
      181 tcagaaccga atgcagggga tggggtatgg gttccaggca gtaggtgttg ctgtccggat
      241 ataccatcta ggatctatta tgacctatat catgctcaag agcagactgt ggtggtaaga
      301 tcgtattgta cattcctatg tctgaaatgt cagaagtatt tttgggaccg ttaaagaaca
      361 gtcttatttg ttcgtgtagc cctatctgaa tccactttca tatcctatgt ttctaattgc
      421 acctgtagcg ttcgctcaac cactatcttg cggtagtatg ttcagaccac aggcattttg
      481 aaaatacctt atatccgtca atatctttgg taaacaaaaa gatttcgagc ttttacacta
      541 tcaggtagcg tgtacggaat gagattttgg agaacaaaac cctctacaca ttcaacctcc
      601 cttttcatgc ccctcagtag aaaagcatga ccacccttta ttacgtgccg gcctcctttt
      661 gtcagaattg acagtgtgct gccaaacgcc ttagcgataa gacactggac atttaacgcc
      721 tcgatcgatt caatacgacc atgaataggg aaaaatgtgt taaactttaa ggtccgctgg
      781 atatgccttt gaaattgaat ctttttaagg gtcctgtcga ttgaaaacac tttcatttcc
      841 ttattcagga ttttgagagg aatggcaagg atgcctgcac ctgaccccat gtcgagaact
      901 gatactgctt tttttacata ggcatagagg tagaatgcat cataaagtag gtcaacaaca
      961 ataacatctt cactttttag acccacgaga ttaatctttc tattccattt tgtaagttcg
     1021 gttacataga ggcagagatt tttgagaacg tccccatcgt aaggtatatc aaatgatatc
     1081 aaaccatgtt tcacagccat ttctaagccc atagataaaa catagcataa tttttttctt
     1141 tctgttataa ttcagcaatg tcgcgtctca ttgcaatcat agcttcatgc ctggtccttt
     1201 tttcctgtac atcgaaacca gttaaaaaat cagaaaaccc cggtagtctc tatgtcgaag
     1261 gggtcaatct gatgaaggca aaaaaatacg acaaggctat caacaaattc tctgatgtca
     1321 gggataactt tccctttgat cccatggcgc ttatctcgac tgttaagctc ggtgatgcat
     1381 attttgaaag gaaaacctat gatctggcct tgatgactta cgaagatttc ttcaaagccc
     1441 atccagagga tgaaaatatt ccctatgtac tcttacgaat cggtgagtgt tatgaaaaac
     1501 tctccctttc tttagataga gaccaggcct ataccctgaa ggcaatcgaa cgatacactt
     1561 atctcatgaa ccgttatcca tcaagcagtt atgcggccaa cgcagaagaa agtataaaaa
     1621 gactcaatca gaaacttgcc gaccgcgagc tctatatagg cgaattctat taccgaacaa
     1681 atcagtataa tgcagccata acccgcctcg aatacctctt aaaaaaatac ccgcacactg
     1741 ttggtttcga caaagccctc tatttcatag ccatgtcgta taaagcgctc ggagatcctg
     1801 ttaaaagcaa tgaatatatc gagaaactcc agaaggaata cccgcatagc aagctgaaca
     1861 aatcatcaaa cagcagaggg gtgcgacaaa ccctcaagtc ttcaaaaaag gaattccctc
     1921 caatagacca gagttcgaca aaaagaagag aaattgactt acgcccccag attattaagg
     1981 cttccactaa aggagaaact gtaagggtta cacctgacaa acaacaggcc ggacagactg
     2041 ataaggacaa gagtgggctc ggtttcattg ataggaccaa gcctatcgat attgtctctg
     2101 ataccatgga aggttttgat aaggagaaga tggtcgtttt taaaggaagc gttgttgcca
     2161 gacaggatga tctctatata ttttcagatg tggttgaaac atttttgaat gaagagtcaa
     2221 acgagatatc aaaagcctat gcaaagggca gtgtaaagat tgtcaaacag gataggacag
     2281 caaccgctca agaagctttt tttgacaata taaagaggga aatcattctc aaaggagatg
     2341 ttgtagtctt ccagggtcag gataaaatca ctggaaatgt gatcacttat tatattaatg
     2401 aagacaaggt tgtagttgag ggagatcctg aaaagagggc tcgagccatc ctaacaccaa
     2461 aacccaaaaa ccagtgaaat gttggacgtg aaaggttgct accccaccag tcatctatca
     2521 gcatttttac tgttgctatg attgttccgc aacgactaaa cgaaatatta catggtactt
     2581 ggttttgggt tctcgcctac cattttctgt gcatagaaac gactcaatac gtaaagaatt
     2641 aagggaaaac acattcccat atttcttacg tttggaaatc ccaaaccttt aaccatttac
     2701 ttcttactgc tttagaaatg gaatttcctc actttttaca tataccaggg cctggcacac
     2761 cgctattaga ttcgggcctt cttgaacggt aatatagtat gatgcagtcc tccgtgttct
     2821 gttaatctcg cgcgattcag cagtaagggt gctgttcttt ttcggtgctc tcatgtacgt
     2881 aacgttcata ttcaaggcca ctgcaacgtt gtcatggctg ttagaggata tctcaaaagc
     2941 ctcgtcaatc agagaaaata ttgcgccgcc gtgggcagct ccataaatat tttcaagcga
     3001 ctcggtaaat tccatctcac agagtgcata tccatcgtca acatccttca gacgaatatt
     3061 caaaagcctg gcaaggggtt cttcctgaac ctttttaaag taaatagcct tcttgctttc
     3121 ttccatttca ggctatgact ttcttgagta gattctctac atttgacttg ggttgggcac
     3181 ctacaagtct gtccaccact tcccctttgt tgaagagaat gagggttggt atacctctaa
     3241 caccatactt tgcaggagta gcctggtttt catctacatt cagtttgaga atctttacct
     3301 tgcctgcata ctcaccggcg atggcatcaa ccacaggtcc cataacctga catggcccac
     3361 accaggtagc ccagaaatca actaccaccg ggatagttga ctccaagact tctttcgtaa
     3421 aatcgctgtc tgttactgct gctgcactac tcatagtaac ctccttcata attataaaac
     3481 tttatgttat cagcttacag cgaataaact acaaattcaa ccattcgaat tgatattcca
     3541 gtctcaaaac tctaatttca agaatatcag ggctttttga tatgcattcc cataacaaca
     3601 ccgtagtccc acggttgtgt actgtagctt ttccatgtca acactctggc attccgatgg
     3661 ctttccctgt tcgagaacaa aatgaaactc aaatccacac ttttatatat gtaatatttt
     3721 tctgttacaa ttacatcaca ttaatggggc acttgttatt taatagcgtt ttttcaccac
     3781 actatcaacg tgagatttaa caacgaagtg ttgtctgttg ctccctgtcg tctattcctt
     3841 aaaaaatttc aaccggaggg aattactcac aacagagata gaactcagca tcatagccag
     3901 cgctccaaac atgggatcaa gccttatacc gaaaaagggg tagagtgctc caaaagcaat
     3961 gggaatgccc agaatgttgt agatgaatgc ccagaatagg ttttctttaa taacccaaag
     4021 ggtctttttg gacagcttga taaggctgac aagttttgag agctgtccct tcataagtac
     4081 cacgtctgcc gactcaatag caatatctgt tccttttccc atagcaacac cgatatctga
     4141 tgctgcaagg gagggggcat cattgatgcc gtcacctacc atgatagtaa cgcctttttt
     4201 cttgaagtct tcaacaaagg cagctttttg gtcagggagg acccggtaaa aaaactgatc
     4261 aatgccaacc ttttcgctga tagtttttgc tccgttctca ctatcaccgg taatcatcgc
     4321 cacatgaatg tccattgatt tcagatcgct aactacatct ttagactcct ctcttacgcg
     4381 gtcactgaag caaagtaagc cgataatact ttctttgctc cacacaagga caggtgagca
     4441 tcctgacaac tcttctttgc ggtagagctc tttcactgac acatccagaa ctctgcccac
     4501 atcctcatat agagttagat tcccaacata atacgtaatt ccattgatgg ccccctttat
     4561 acctttgcca actatggcct gaaactcatc aacattacta atttcaattt tcagctcttc
     4621 tgcttttttt cgtaatgcct ctgagaaagg atgttcagac tgcctttcaa ggttatatga
     4681 aacaaatagg agctcctctt ctgaatgcct accaagtggt atgctttccg caagaacgat
     4741 gaccccctcg gtgagggtcc cagtcttgtc aaagagaacg tatctagccc tgttcgtaag
     4801 ttcaagggct tcagcactct ttattagtat tcctctcttt gctcctactc ctgttgaaac
     4861 cataatagcc gttggggtag ccaggcccag ggcacatggg caggcaatga tcaatacgct
     4921 aacaaaggaa agaagcccgt ttgtcaactt ggggtcaggt ccaaaaaagt accagacaat
     4981 gaaggcagca atactgataa gaataactat gggaacaaaa actcctgcta ccttgtcggc
     5041 aagtctctga accggcgctt ttgtaaactg cgcttcttca acaagtttga ttacctttgc
     5101 aagaacagtg tcctttccta ttcttgtaac cctcaccgta actgaaccct taccatttat
     5161 ggttccacca attacctcat cacccacatt tttatgaaca ggcaggcttt caccggttat
     5221 catggactca tccacatagg tatttccgtc tatcacttcc ccatctatgg gcaccttttc
     5281 tccaggcctt atcaggataa ggttcccaac ctctatagta tctgttggaa cttttgtttc
     5341 ctttccttcc tgaagaagaa cacactcttt gggagaaagt tcgtaaagca ttttaattgc
     5401 cgtatatgtc cttgtttttg ctttagattc aaaaaatctt cccaatagga tcagagtaat
     5461 aatagtggca cttgagtcaa aataggtcat tggttccagt cccaccttca taacagtgtg
     5521 aggaaaaaag gtaacaaaaa cgctataaaa gaacgctgct gaggttccca cagagatgag
     5581 ggtgttcatg tcggcagtca gatgcttcag attgaggagc gttgctttat gaaatcgtag
     5641 cccaaagtaa aactgtacgg gcaatgtgag tatgagcaga ataaagttct gatattgcat
     5701 attggggagc accatccaca tagaaaaaac catgacaatg gcgctaaaac aggctgacca
     5761 tacaaaatct cgcttaagct ccgcttcctc ttttcttgct cgtattgtaa cgtccgcttc
     5821 aatgtctaca tcataaccga tgtcacggat aagagaaatg atctgcttta gttcaagctc
     5881 ctttttcggg acaataactg ctttttttaa cggaaagtta acccgtgctt ctccaatatc
     5941 ttccagttta ttaagttctc tttctattct tgccgcacac gcagcgcaac tcatacctgt
     6001 aataggtaat tcaatttttt caggcatttg accctctcgt tgtgtagtaa ttcaactttc
     6061 aaaatataaa gcaggatggg ctaaatgaca acgtaatgcc aatactcgcg gacatccctg
     6121 tgaagcagaa acattgatgt tgcctgtttc aaaaaagcaa cattcacccc ccctcaggaa
     6181 ccctatccct tccttgacgt acgcttcttc cgggaagctg tctatgatcg tcgaggcgac
     6241 aacgaggggc tattgagggt gtgccggcag tgtaggtgac ataagcccta gccaggaatt
     6301 attttataaa atcaggcact caaagaggca ggggtataaa agaaatggta aagtgatttg
     6361 attgccttac tggtccttgg agattcaatt tttgaccaat cgctcttgat gcctttgaga
     6421 agtgaagcct tctttccatc aagaagtgat gaggttccca attgctggtc tctcgtatca
     6481 aggtccacag ctggaataac tcgatagagc ctgttttctg catcaaactc cacaacaccg
     6541 agtgtatgcg aaaaatcatt atagccttcg aatgaaagcc ctgactgttt tctgcgctct
     6601 acatagtcct tgaggactga cttgtcttta ataccaatag atacccacag gacaatcttt
     6661 tccgggagag gcctataaca atcagcagat ggaaatatgc cattatcaaa ttcctcagaa
     6721 cccgcaacat tgtctatatc gcaaaagaga ttgaaggttt catcctcata gataagtttc
     6781 cagctccaca tcatggtaat cctccatcac gttaaaattt gaaaacacta taattagact
     6841 aaaagcatca acccgctcta tgcaggaaca tacattgaat tttgacctgc actcattata
     6901 acccatgtac ctggttttac caaagacatt ccattcgtac catgggtgat cgaggacctt
     6961 gccaacactc catactatcc gggcaaaacc gtttcaatct tcagtcatgg agctcatatc
     7021 tatcattaaa taaaatagca tcccacggct tcctctggag attctataca ttctaatatc
     7081 ttctttaccc gccacatcca caagtgtcca tgtatcttgt ctgacttgac catctctatt
     7141 tacactacat tccaacaact attgcgagta tttgcatctt ctaatcctac ggggaatgga
     7201 atcatgtcat actttctttt ctatgccagt cgagtattct tttcgcccat gctggaaatc
     7261 cttaagaaat tttgactttc ttctaaatca gcatgattat attctaaact ccataaatat
     7321 agatgcttcg gtagcttcgg atattcctcg agggaatctg aaattagctt cccctttctt
     7381 agtctcattg tgtagccata tcatccggtt ttgagggaac aattttagat ataaagtcac
     7441 tgtgaattac actgtaatat gattggtttc tgatctagct atccaaatct gtgttctttt
     7501 ttgccattcc gtctgctctc tcattataac aatatttact gatgagacat aaaactgttt
     7561 cctgcttgaa agcaccttaa ccctccttga caaagtaatg ttgttaccac actatattgg
     7621 tgtgaatttt aaactggaac gagtgtattg ctgaatgtct aacacataca attttactgg
     7681 gaggtatgaa ttatgtatac agcaaaggac tactcaaagc ttatcggcat ggagggattg
     7741 agcgataccc ttctcaaaaa tcactttact ctctatcagg gatatgtgac aaacacaaat
     7801 aaaatcattg aattacttgc ccaaatgctg aaggaaaata aagaaaacac ccctgaatgg
     7861 gcagaattga agagaaggat gggctgggag tttaatggca tgagacttca cgagtattat
     7921 tttgagaata tgggcgggaa agcctctctc aacaaagatg ggaaactgtc caaaaaaatg
     7981 gctgaagatt ttggaagcta tgaaacatgg gaaaaggcat tccgagcagt cggatcaatg
     8041 aggggaattg gatgggcggt actttaccag gacaatacta acggaagatt gattaatttc
     8101 tggattaatg aacacgacgc aggacaccct acggggtgca gtcccttact ggtcctcgat
     8161 gtttttgagc acgctttcat gattgactat ggcctcgggc ggacaaacta tatcaatgcc
     8221 ttcttcaaga acatcgactg ggcagtggta gaaggcaggc tgaaataggt ttctcataat
     8281 catgagcaaa aattgttaat tcaatctatt ttggggttga aaacctcttg ttttgattga
     8341 caattggcac aacagaggct gtgtttgggt agcgtgatga ataaaaagaa acaatcagtt
     8401 tttttctcga cagtatactg ggtatttcta gttgctgctc tttcatctca cccttctgta
     8461 tctttcgcag aggaaagttc gggaaataaa actttcgaag caattctgag caatggactg
     8521 aaggttatcc ttcttgaaaa tcacaaggca ccagtagtaa ctgtgcaagt ctggtaccgc
     8581 gtcggttcac gtaatgaaac ctgggggaaa acaggcctat cgcatcttct tgaacacatg
     8641 atgttcagag gcagcaaacg gttcagtgct gagcaatttg tccgcatgat tgaagaaaac
     8701 ggcggaaaaa gcaatgcatt tacctctcat gattacactg catattttgt taacatctgt
     8761 gcagaacgcg ttgcaattcc cttggagctt gaagcagaca gaatgcaaaa tgccacattt
     8821 gatgaaaaaa agttcgcaac agaaaaaaag gttgtcattg aggagcgcag actgcgcacg
     8881 gagaataacc ctcaggcata cttatatgaa cagatggcgg caacagcata ccaggttcat
     8941 ccataccact ggccggtcat aggctggatg gaggaccttg aggggcttac cgtttcagat
     9001 gcattggact actatcatcg ttattacgta cctgcaaatg cctttcttat agtggtcggt
     9061 gatttttccc gacaggattt acttcctaag atcgaaaaag catttggctg tcttccttca
     9121 actcaagctc cacaacaggt tgtaagtcgt gaaccaattc aaaaaggtga aaggcggata
     9181 gtcataaaac gagaaggcct tgtacctgcg ctgatgattg gctatcatgt cccaaatctg
     9241 cataataccg atggttatgt tctcgaggtc atcgagggaa tactctcatc aggaaaaagc
     9301 tcacgcttct ataatcgtct tgtaagagaa aaacaaatcg cgctcagggc ttatgcagat
     9361 ttttctctta cttctttcga cccaggcctc tttttcatct ctgcggaagt gttagttgaa
     9421 aaggacttga caacattgga aaaagctata gatgaagaga ttataaggct tcaaaccgaa
     9481 cccgttgcaa tggaagagct gaataaagtc aagaatcaat tggaagcggg ctttatccat
     9541 agtcaggatt cagtttttaa tcaggcaatg gtcctcgcta ggaatgaaat agctgcaacc
     9601 tggagatgca ttgatgatta tattcccgct attcatcgtg taacacccaa tgatattcta
     9661 agggttgccc gtcaatacat gattccagaa aacagaactg tgggctattt cataccactg
     9721 cctccacaaa acatagaatc gaacaactct aaaaatctta tcgtggagga ggaatacacc
     9781 aggtgaacaa cgttaaaccg cgaagattga gtccatactg gtggccgtta aactttattt
     9841 tcttcctgat attgtttccc agtcattctt tttctcttcc tcctacagag cggatgagtc
     9901 ttcagaacgg ccttcatctc attgtgagcg aggctcactc ccttcccttt gtgacatttc
     9961 aattaataat tgatggcggc tcacgcttcg acccgccaaa caaagaaggg ctttcctacc
    10021 taactgcaaa agggcttctt cacggcacca caaaacatac atccaaacaa atgaacgagc
    10081 ttttggattt tatgggcgcc aatctccata cggacagcag taaagattac atcactgtgg
    10141 gttttaaggt gttgaaaaag gatctttttc aaggattcca tctttttatg gacgttatta
    10201 cactaccggt ctttcctaag gacgaattcc atagcgagat acagagaacg cttggtatca
    10261 ttaaatctgt tgaagaagat cccaaagaac tttcggaacg ggaatttttc aaaactctat
    10321 tcgccggtac cccctatggt catccagtta taggaacaga ggagtcaatt ttaaaattaa
    10381 cacgcaatga tcttgcagca tttcacagct catatgttcg tcccaacagg gccattttgg
    10441 taataacggg tgatatcagt ctaaatgaga tcaaaatgga ccttatgagc aggcttgaag
    10501 catggcctgc aggaaatgtt ctcccgactt cacataaaca accgtctgca caaaacacta
    10561 agatactcac cttagagagg gcaataagcc aggctaatat taccttgggt aaccttgcac
    10621 cacaaagaaa taaccctgac ttctttccca tgatagtgac gaactatatt cttggcggcg
    10681 gcttttcgtc aagactttta actgaaatca gggcaaaacg gggtttagca tattctattc
    10741 gcagcacctt tcatttcaag aagcgtgtcg ggtcgttttt ggtcagcctc cagacaaaga
    10801 atgattcagc ccaagaagcc atatccatcg ttctcgaaga aataaagaag ttacagaaag
    10861 agccggtgac cgatattgaa ctggagggtg cccgcaaata cctgacagga agcttccctt
    10921 tgcagctaga cacccaagcc aaacttgcag gttttctcgc tcaggtggag ttttatggcc
    10981 taggagaaga ttacgcccat aattatgtgt cctgtatcat ggctgttaca aaggaagatg
    11041 ttcaacgggt tgcaaagaaa tatcttaacc cgtacaatta taccctcgta attgttggaa
    11101 atctcaagga gggaagattc agaaagtaaa tcttaacaat cctgacacaa ttcggtttag
    11161 ggtataacac tatttttaac aacatggagg atgtggaagt gttacatgca cagtcttttt
    11221 tttacagaaa acaaaataaa ataattattg ttttttcatt tgcatggttt ttaatgttca
    11281 ttaccgtgcc tggtttttca ccgggtattg agcgagtaaa atccccaata gaattaagca
    11341 cggccatcat tcaagtagca aagaaaaata tacctgcagt tgttcacatt ggggtaacgg
    11401 gacaacaaga agtggcaacc ccttcacttc catttaatga tgacccattc tttcgtcgtt
    11461 tttttggcat accacaaggg ccacgaaaat ttaaaagaga aacaaggggc cttggcacag
    11521 gcatgatcat tgacagtcag gggcatgttc taaccaacta tcacgttgta ggtaatgcag
    11581 acaagattga tgtactcctt tcagacggga gttcatttca cgccaagtta gttgggtctg
    11641 atcctaaaac agaccttgca gtcattaaaa tatcggcaaa ggaaccgctt ccctttgtga
    11701 cttttggtga ttcagacaag gtggaggttg gtgaatgggt tgtagccata ggtcatccca
    11761 ggggattgga tcagacagtc acccaaggca tcattagcgc caagcatagg cgaggaatca
    11821 cagacccttc cagctaccag gattttttac agactgatgc tgccatcaac cctggtaaca
    11881 gcggtggccc acttctcaac cttcggggcg acgttgttgg agtaaatacc atcattgttt
    11941 caggctcagg tgggtttgaa gggattggtc tgtcaatccc aagcaatatt tcccgacatg
    12001 tggcaagact ccttattgcc catggaaaag ttgaacgggg atggattggc ataagcgctc
    12061 aggacttgac ccctgaaagt gcaaagaatc ttgccacaga aaccagaaag ggcatactca
    12121 ttggagatgt tgtaaaaggt ggtccggcgg acaaggcagg tatcaaaaag aatgatgttg
    12181 taatagcatt tcaaggtaaa gaggtctcca atgctgcaac actgcgaaat gaaatctcat
    12241 tgagtccaat cggcaaggac attcgaatgg taattctccg tggaggaagg aagcaagaag
    12301 ccactgtccg aataggagat tcaaaagatg cggttaaagc actcagcacg tctgtccgtg
    12361 aacgactcgg ggtcgatgtg agatcattaa ctcagaaaga agctgacaag ttccggttga
    12421 acattaatga aggggtatta gtaacattcg tggaccaaaa gggccctctc ggtagggttg
    12481 gatttgaggt gggagacatt atccttgaaa taaacggcca atcggtaggt ggaatagaag
    12541 gattttctca gatgattgca tcacttcgtc ccaaccaacg tataacatta cttgcttttg
    12601 atcacagttc aggtaattct ggttatgtga atgtgacatc acggtaaagt atgtatggtg
    12661 aaaatcaccc cgaataatgg tggctatctt ctatactagc ggtcagatta ttttctggct
    12721 taactgaaaa acccctcaaa gatgttccta tgattgcagc aaacaatata taaagattca
    12781 tcatacattg ctgaagaatg gcttcatcat atgtaacatg ctatacttat cagagataat
    12841 aaaggaggtt tttgatgaaa atacatgcaa atgatgtaat tgtgcctttg gatgtgccaa
    12901 aagcgtttca ggaaacttat acacgcaatt atcttgaaat taccagagag agcgggcacc
    12961 ttatgctctt tgcgggggat cagaaggttg agcatctgaa taaagacttt tttggtgagg
    13021 gtatccctgc ggacgataat gatcctgaac atctttttcg gatcgcaagc gaagcaaaaa
    13081 tcggtgtatt tgcaactcaa ctaggtatta tcgcccgata tgggatgagt taccctgctg
    13141 taccctatct tgttaaagtc aacagcaaa
//
LOCUS       DAOOMC010000081        13153 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdus sp. isolate AMR_MDS_2685
            NODE_5214_length_13153_cov_6.003588, whole genome shotgun sequence.
ACCESSION   DAOOMC010000081 DAOOMC010000000
VERSION     DAOOMC010000081.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864760
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdus sp. (wastewater metagenome)
  ORGANISM  Syntrophorhabdus sp.
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae; Syntrophorhabdus.
REFERENCE   1  (bases 1 to 13153)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13153)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,729
            Genes (coding)                    :: 2,708
            CDSs (with protein)               :: 2,708
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 7 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13153
                     /organism="Syntrophorhabdus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5214_length_13153_cov_6.003588"
                     /isolate="AMR_MDS_2685"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2699756"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1799)
                     /gene="secA"
                     /locus_tag="PK125_09105"
     CDS             complement(<1..1799)
                     /gene="secA"
                     /locus_tag="PK125_09105"
                     /inference="COORDINATES: protein motif:HMM:NF009538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HPB38302.1"
                     /translation="MITNILKKIIGTKNERELKRIQPLVEKVNSLEDEYRSLSDEQLK
                     AKTPFFRERLEQGEELDMLLPEAFAAAREAAQRTVGMRHFDVQLIGGVVLHEGKIAEM
                     ATGEGKTLVATLPVYLNALTGLGVHVVTVNDYLARRDAEWMGPIYKFLGLSVGVIVHG
                     LDDDERRNAYVSDVTYGTNNEFGFDYLRDNMKYSMDECVQREFNYAIVDEVDSILIDE
                     ARTPLIISGPAEESTDKYYKINRLIYQIKKDKDFMVDEKAKSAYLTEEGVARIEKIIN
                     VDNLYDPKYVDLLHHINQALKAHNLFQRDVDYIVKDGQVIIVDEFTGRLMPGRRFSDG
                     LHQALEAKENVKIERENQTLATVTFQNYFRMYKKLAGMTGTADTEAVEFKKIYNLDVT
                     VIPTNRPLIRTNYADVVYRTEKEKFKAAIAEIEDLYKKGKPVLVGTLSIDKSERVSEI
                     LKRKGIPHHILNAKNHEREAEVIAQAGRSKAVTISTNMAGRGTDILLGGNPKFLALSM
                     AKGKTDTEEYENILEEAKKICEKDKEEVIRLGGLHILGTERHESRRIDNQLRGRAGRQ
                     GDPGSSRFYVSLEDEIMRLFGSDRVSPMLAKLG"
     gene            complement(1951..2421)
                     /locus_tag="PK125_09110"
     CDS             complement(1951..2421)
                     /locus_tag="PK125_09110"
                     /inference="COORDINATES: protein motif:HMM:NF012905.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HPB38303.1"
                     /translation="MISVSIPGWGDLDIEYMVIDYNGTVAVDGKPKDGVKELLEKISR
                     YIKLFIITSDTYGNIDNEGTTVGFRIIKVGKEGSGREKARIIKELGPEKVVAIGNGNN
                     DALMLKEAALGIGVMGEEGCANILTKEADLIVKNVVDALNIILHPERLVATLRD"
     gene            complement(2648..2803)
                     /locus_tag="PK125_09115"
     CDS             complement(2648..2803)
                     /locus_tag="PK125_09115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB38304.1"
                     /translation="MKRKKQKKVTKTEQEVKRVENALCDGQTSITDYASRSLAITHNS
                     PMPDLQT"
     gene            2943..3596
                     /locus_tag="PK125_09120"
     CDS             2943..3596
                     /locus_tag="PK125_09120"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HPB38305.1"
                     /translation="MVKPKWEVECVIYDCDGVLFDSLDANSRLYNYIATSTERGPLTP
                     DELWYCHTHTVYESITHLFKHDGKLEQKARDLLKTVNLGDFVVYLKMEPNLIRTLTEL
                     KERNIGRAISTNRTTSMKYIMERFKLDPYFEMVVTALDVKHPKPHPESVEKILTTLKY
                     DREKTLYIGDSGVDRETALSSGVKFIAYKNKEIAEHGFIEDHLTLLDFLSGGEHSQE"
     gene            complement(3535..4302)
                     /gene="pssA"
                     /locus_tag="PK125_09125"
     CDS             complement(3535..4302)
                     /gene="pssA"
                     /locus_tag="PK125_09125"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003882 - CDP-diacylglycerol-serine
                     O-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HPB38306.1"
                     /translation="MKRRKHKGIYILPNLLTSISLFAGFYAIIASINLKFSHAAYAVF
                     VSGIFDMLDGRVARLTGSTSRFGVEFDSLSDAIAFGVAPGILVYMWALKGYGRFGWLA
                     AFLFVACGALRLARFNVQADNAQKRSFLGLPIPAAATTIAGCVLFYSWLGYSDGIKTV
                     FMPILVYLLALLMVSDIRYYSFKDMSFFRGRPFRSTVAAILFLVIIFIEPEVTLFLVT
                     SLYVLSGPIYTLLAKKKIILENAPRPKENQAGSDGLL"
     gene            complement(4299..4943)
                     /locus_tag="PK125_09130"
     CDS             complement(4299..4943)
                     /locus_tag="PK125_09130"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: protein motif:HMM:NF003678.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase family protein"
                     /protein_id="HPB38307.1"
                     /translation="MKEALIAKEGLKLIIPSFVLSIIFYIFFYYSLAVLSILFFLFCL
                     FFFRNPRRSSREPMDRLISPADGKVTEIKDIVENEFLGYEAIRVSIFMSLASVHVNRS
                     PMAGRIMQVVHRPGKFALAWKADVDQENERNYILLSGNNEKILVVQIAGFLARRITSY
                     VHAGDTVARCEPLGIIAFGSRVDIYFPKSYECMVKLHEMVTAGKTPLARKRGTS"
     gene            complement(4940..5938)
                     /gene="ilvC"
                     /locus_tag="PK125_09135"
     CDS             complement(4940..5938)
                     /gene="ilvC"
                     /locus_tag="PK125_09135"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: protein motif:HMM:NF009940.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HPB38308.1"
                     /translation="MAKMYYDKDADLGVLKGTKVAIIGYGSQGHAQAQNLRDSGVDVI
                     VAELTGTPNYKIAVDHGFKPVSASEAAKKADMIQILAQDNLQAQLYKTEIKDNLKKGK
                     TLVFSHGFNIHYGQILPPDDVDVIMVAPKGPGHLVRREFERGAGVPSLIAVFQDYSKK
                     AKKKALAYAKGIGATRAGVLETTFAEETETDLFGEQAVLCGGASELVRAGFDTLVEAG
                     YQPEIAYFECLHELKLIVDLLYEGGISYMRYSISDTAEYGDMTRGRRVVSEETREEMR
                     AILQEIQSGEFAREWILENMAGRPVYNAIKRIDNEHLIEKVGKQLRSMMGWIGRKG"
     gene            complement(5931..6458)
                     /gene="ilvN"
                     /locus_tag="PK125_09140"
     CDS             complement(5931..6458)
                     /gene="ilvN"
                     /locus_tag="PK125_09140"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HPB38309.1"
                     /translation="MRHIISVLVENEFGVLSRVAGLFSGRGFNIESLCVAETLDPTIS
                     TMTIVTSGNEAIIEQVLKQLNKLINVIKVVDFKELDYVSREMVLVKVNAEERTRAEIL
                     RMTEIFRGKIIDVSPKTYTLLITGDDEKLRAFLTLIKPLGIKELVRTGPIAIARGEKV
                     IKLKDKPLEGGEEDG"
     gene            complement(6469..8163)
                     /gene="ilvB"
                     /locus_tag="PK125_09145"
     CDS             complement(6469..8163)
                     /gene="ilvB"
                     /locus_tag="PK125_09145"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005948 - acetolactate synthase complex
                     [Evidence IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic-type acetolactate synthase large
                     subunit"
                     /protein_id="HPB38310.1"
                     /translation="MKKTGAQIFVEALKIEGVENIFCYPGGATLNITDALTDHSEIHQ
                     IVVRHEQGAVHASDGYARASGKVGVSLVTSGPGATNTVTGIATAYMDSIPIVIFTCQV
                     NTMLIGNDAFQEADIVGITRPCTKHSYLVKDVKDLARIIKEAFYIARSGRPGPVLVDI
                     PKDVSSGICDFKYPEKVNIRSYQPTYNGHLGQIKKAIKQIAASKKPVLYTGGGIITSG
                     ASAELVKFAEQLSIPVVNTLMGLGGVPGGHKLFLGMLGMHGTYAANMAITSSDLIIAI
                     GARFDDRATGKVDEFAPHAKIIHVDIDPTSISKNIKVDIPIVGDSRSVLKKMVEIVEA
                     EKEEFKAYKASIGPWLNETEQWKKNFPLTYVRNGTLKPQYVIEKVFELTDGKAIIATE
                     VGQNQMWTAQFFKFLKPRTLLTSGGLGTMGFGFPAAIGAQVAFPKSLVVDIAGDGSIQ
                     MNIQELGTAVQFNLPVKVVILNNGYLGMVRQWQELFYEKRYTWTCLECAPDFVKLADA
                     YGAAGYRIESEDEVESVLREAFHNGRPTLIDVKVNPEESVYPMVPAGASLKEMLLV"
     gene            complement(8160..8369)
                     /locus_tag="PK125_09150"
     CDS             complement(8160..8369)
                     /locus_tag="PK125_09150"
                     /inference="COORDINATES: protein motif:HMM:NF016231.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF465 domain-containing protein"
                     /protein_id="HPB38311.1"
                     /translation="MEQSESKEDIYNRAKTQHATLDRRLQMLMKKPYLTDDEEMEVRI
                     LKKKKLYFKDIMERVGEEPKKGEKD"
     gene            8589..10307
                     /locus_tag="PK125_09155"
     CDS             8589..10307
                     /locus_tag="PK125_09155"
                     /inference="COORDINATES: protein motif:HMM:NF014425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HPB38312.1"
                     /translation="MYWSGHKGLKNLIYLVLIVVPLFSFGLTNHGLWSADEPRVAEIG
                     REMALTGNWAVPMLNQRPFLEEPPLYYATLALTFKALGVSDRVARIPSAIFAFATVLV
                     LFFTVNFLFGPQIALFSGIILATTGEYFRVAHTVIVDSALTFFIACAMSLFIVGYLSD
                     SNRKKLLCYILLYISCTFAFYTKGFIGIVIPGLGILSFLVADKNIKEIIKMRLWLGIL
                     IFLVLTLPWFITLWQQGGKEHLDVFLLHNHLQRFLPGKFSGTISGEASGHHHPFYYYL
                     IEFPVGFLPWSLLLVPVFIHAFSRSDKSETPPEKGRLLAKCWFFAGIVFLSAASTKRT
                     LYLMPIFAPMALLTSLYVESMLYRLADKIEKTFLWAFTAIFAVIGISAVPVYSSIQGI
                     YPWIRGTNLFPSTVLLSLLIVPSSVSAMWFLWSKNFGKYWVTTIIPIVVGMVFTAVAV
                     APVVDIHKSFVPFCHELMAIVPSSEPLYAYSADETLRGAVPFYTGRYLTEICGPGNLE
                     KTIETKGQVFIITRDKKRKAETELLSTGKLSIIFQQKMGGERTLTLLGKNSHTVQSDI
                     HLAKDN"
     gene            10450..12144
                     /locus_tag="PK125_09160"
     CDS             10450..12144
                     /locus_tag="PK125_09160"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HPB38313.1"
                     /translation="MIALPGDLRKRKAIYLVVIIAASYLFFFYNLNSYSLKEPDEGRY
                     AEIPREMVEQGNYLVPHLNYVRYFEKPPLLYWITAASYKVFGINEWSFRFPNALAAFF
                     CSILLYLFSCKRFSPKTGFISSLILISCFGFFTMARIVTIDMLFTCLLFGALLSFHEY
                     YLTHKGLFFYLFHICLSLATLAKGPAALILLGVTILIYLRTEKRLSFLKDLLSYRAIL
                     IFAIITMPWFVIMSIKEKEFFHFFFLDQNVLRFLTTRHNRSGPLYYFIPVLALGMLPW
                     SVFLPRVIIRLWWVRELRLFFIWSAVVFAFFSISGSKLPPYILPVFPALAQILGYFFA
                     TRQQDSVPANCEVIAYFVFFATVMMAGFLGASGILGKYLQDELYIQDILPDIWGLELG
                     IALVSAVMICFMCIRSMRRFGPFFYALTTFSLVIVIALMVNTRVIDKHTTTKGLAKTI
                     NNFQSTPAYIVNYGSFEETLPFYTRQRIHIACYKGELAMGSKYPDARPFFLDQAAFRN
                     LLDSGQPVFVVIKKKYLSSAAKTTNQELKLLDCQNERCLAANQNVQVFYPELAFYR"
     gene            12660..13022
                     /locus_tag="PK125_09165"
     CDS             12660..13022
                     /locus_tag="PK125_09165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB38314.1"
                     /translation="MFYQKMNEKGAGGKYENLNYYYDKESIEKPSKGIARVWFKTTVG
                     DDDSENLAEKDGSDESEQYRGHIEINCKTKSYRMIEETSSDSSESEGKGLQPSAGKAP
                     RRLYLDSALGALWSNLCE"
BASE COUNT         3412 a         3233 c         2696 g         3812 t
ORIGIN      
        1 attccaagct ttgcgagcat gggagaaacc ctgtcggagc caaagagcct cataatctca
       61 tcttcgagag acacataaaa tctcgatgat ccgggatctc cctgccggcc tgcccttcct
      121 cttaactggt tatcgatccg gcgcgattcg tgcctttctg tgccgaggat atggagacca
      181 cccagcctga taacctcttc cttgtctttt tcgcagattt tctttgcttc ctcaaggata
      241 ttttcgtatt cttcagtgtc tgtctttcct ttggccatac tcagggcaag aaattttgga
      301 ttgcctccca gaagaatgtc agttcccctc cctgccatgt ttgtggagat tgtaaccgcc
      361 tttgatcgcc ctgcctgggc tatgacctcg gcttcacgtt cgtggttctt cgcattgagg
      421 atgtggtgag ggatgccttt tcgcttcagt atctccgaca ccctttcaga tttatcaatt
      481 gagagggttc ctacaaggac cggctttcct tttttataaa gatcttctat ctccgcaata
      541 gcagctttga atttttcctt ttctgttctg tataccacat cagcataatt ggtccggata
      601 agaggcctgt ttgtaggtat aactgttacg tcaaggttat agatcttctt gaattccaca
      661 gcctcggtat cagccgtccc tgtcatgccg gcaagcttct tatacatcct gaagtagttc
      721 tggaaggtta ccgtggcaag ggtctggttc tcccgctcga tctttacatt ctctttggct
      781 tccagagcct ggtggaggcc atcgctgaac cgtctgcccg gcataagccg cccggtaaac
      841 tcatcaacga ttatgacctg cccatccttc acgatataat ctacatctct ctgaaaaagg
      901 ttgtgggctt ttaaagcctg gttaatatgg tggaggaggt caacgtattt cggatcataa
      961 agattgtcta cgttaatgat tttctcgatc cgcgcaacac cttcctcggt gaggtaggcg
     1021 ctctttgctt tctcatccac catgaagtct ttgtcttttt tgatttgata gataaggcgg
     1081 ttaattttat agtatttgtc agttgattcc tcagcaggac ctgatatgat taaaggcgtt
     1141 cttgcctcat caatgagaat actgtcaacc tcgtcgacaa tggcatagtt gaattccctc
     1201 tgaacacatt catccatgga gtacttcatg ttgtccctga ggtaatcaaa tccaaactcg
     1261 ttgttcgtcc cgtaggttac atcacttaca taagcattcc tccgctcgtc atcatccaga
     1321 ccgtggacta tcaccccgac tgagaggccg aggaatttgt aaatagggcc catccattct
     1381 gcgtcccttc tggccagata gtcgttgacc gtgacaacgt gaacaccgag accggtaagt
     1441 gcgttgagat aaaccggtaa tgttgcgacg agcgtcttcc cttctccagt ggccatctct
     1501 gcaatcttgc cttcatggag caccacgccg ccaatgagct gaacatcaaa gtggcgcatg
     1561 cccacggttc gttgtgctgc ctcccgtgcc gcagcaaatg cctcgggcag aagcatgtca
     1621 agttcttcac cctgctcgag tctttcccga aagaatggag tttttgcctt gagttgctca
     1681 tctgagagag atctgtattc gtcttcgaga gaatttacct tttcaacaag aggctggata
     1741 cgtttcagct cccgctcgtt ttttgttcca ataatcttct ttaatatgtt tgttatcatg
     1801 gctgaatatt atacctgcgg atgtttcaga ctgtcaatgc aaggaatctg cttatatggc
     1861 cagccccctt gaatgggctg gcacagtgtt ttttcgttct tgagtcacct gacacaaccc
     1921 atcgcgcatc acgggtcacg gatttttatg ttagtccctc agggtagcta caagcctctc
     1981 aggatggaga atgatgttca gggcatccac aacattcttg acgatgaggt ccgcctcttt
     2041 ggtaaggatg ttcgcacagc cctcttcacc cataacccca atacccaggg ctgcttcttt
     2101 taacataagt gcgtcattgt tcccatttcc tatggcaacc accttttcag ggccaagctc
     2161 cttgataatt cgggcctttt ccctgccgct gccttctttg cctactttaa taattctgaa
     2221 tcctactgtg gttccttcgt tatcaatatt tccataggta tcagatgtaa tgataaatag
     2281 ttttatgtaa cgagaaatct tttccaacaa ttccttaacg ccgtcttttg gtttaccgtc
     2341 aacagccact gtaccattat agtcaataac catatactct atatcaagat ccccccaacc
     2401 aggaatggac acacttatca tacatacctc ctcgaatttt atcagtccgt attctaccac
     2461 atttcacacc aatttaaata taaattttag gagttacaca cttctggggt ccatgtctcc
     2521 ccctccatcc agaccatttg atctgtttta acggttcaaa ccctttgaac aacaacagtg
     2581 attcttcccg tctcactcaa aaatgggctg tttaatactg gagttgtcct tacaattctt
     2641 agctccttta agtttgcaag tcaggcatgg ggctattatg ggttatggcc agagaccgtg
     2701 acgcgtaatc agtaatcgat gtttgaccat cgcacagtgc gttttcaact ctctttactt
     2761 cctgctctgt ctttgtcacc tttttttgct tcttcctttt cacgctaacg gcttcttttc
     2821 tctttgaatt ttgttcaata cagggtagaa tcatggggaa ggaaccgagt ttcaggttgt
     2881 gagtttcgag ttcagagaca aacctaaaat ctcagaaatc aacatcagga gaatgttcag
     2941 gtatggtaaa accaaaatgg gaagttgagt gcgttatcta tgactgtgac ggggtgcttt
     3001 ttgattccct tgatgctaat tcgcgcctct ataattacat tgccacatcg acagaaagag
     3061 gaccgctcac tcctgatgaa ttatggtact gtcatacaca tacagtgtat gaatcgatta
     3121 cccatctctt taagcatgat ggcaagctcg aacaaaaggc acgtgatctg ctcaagacgg
     3181 tcaatcttgg tgattttgtt gtctatctca aaatggagcc aaacctaatc aggacattaa
     3241 ccgaattgaa agagcgaaat ataggaaggg caatcagtac caaccgcacc acatctatga
     3301 aatatatcat ggagcggttt aagctcgatc catactttga aatggttgtt acagctctcg
     3361 atgtaaaaca tccaaagccc catccggaat cagtagaaaa aatactcacc actctgaaat
     3421 acgacagaga gaagacactt tacattggag actctggagt ggacagagag accgcgcttt
     3481 cctcaggtgt gaagttcatt gcctacaaaa ataaagaaat tgcggaacat ggttttatag
     3541 aagaccatct gaccctgctt gattttcttt cgggcgggga gcattctcaa gaataatctt
     3601 ctttttcgca aggagcgtat agatcggtcc tgacagaaca tagagtgagg ttaccagaaa
     3661 aagcgttacc tctggctcaa tgaaaattat tacaagaaaa aggatagctg caacagtcga
     3721 tctgaaaggc cttcccctga aaaacgacat gtccttgaag ctgtaataac ggatatcact
     3781 gaccataagc agagcaagaa ggtagacgag tatgggcata aagaccgtct tgattccgtc
     3841 agagtagcca agccaggaat aaaaaaggac acaacctgct attgttgtag ctgcagccgg
     3901 gatagggagg ccaaggaaac ttctcttttg agcattatca gcctggacat tgaaacgggc
     3961 aagccgtaaa gctccacacg ctacaaacag gaaggctgcg agccaaccaa aacgtccata
     4021 gccttttaat gcccacatgt atacgagaat accaggggca acgccaaaag caatggcgtc
     4081 tgaaagagaa tcgaattcta cgccaaagcg gctcgtagat cctgtcagcc ttgctactct
     4141 cccatcaagc atatcgaata ttcccgaaac aaatactgca taggcggcat gggaaaattt
     4201 caggttgatg cttgctatta tggcatagaa acctgcaaaa aggcttattg aagtcaacag
     4261 gttaggaagt atataaatgc ctttatgttt tcttcgtttc atgatgtccc cctttttctg
     4321 gccaagggtg ttttccctgc tgttaccatc tcatgtaatt ttaccataca ttcgtatgac
     4381 ttcggaaaat aaatatccac ccgggaacca aatgcaataa tacccaatgg ttcgcatctg
     4441 gccaccgtat caccagcatg cacatatgaa gtgatcctcc gtgcaagaaa acctgcaatt
     4501 tgaaccacca gtatcttttc attatttcct gaaaggagaa tataattcct ttcattctcc
     4561 tggtccacat cagctttcca ggcaagggca aatttacctg gccggtgtac aacttgcata
     4621 atacgtcccg ccatgggaga tctgtttaca tgaacactgg caagagacat aaaaatactt
     4681 acccgtatcg cttcatagcc gagaaattca ttttccacga tgtcctttat ttctgtcacc
     4741 ttcccatcag ccggagaaat aaggcgatcc atgggctcgc gggatgacct tctcggattg
     4801 cgaaagaaaa aaaggcagaa taagaagaat aaaatactca gaacagccag ggagtaatag
     4861 aaaaatatgt agaagatgat cgaaagaaca aaagaaggga tgatgagttt cagcccttct
     4921 tttgcaataa gggcttcttt cacccctttc tcccaatcca ccccatcatt gacctgagtt
     4981 gtttccctac tttttcaatc agatgttcgt tatcaattct tttaattgca ttatatacgg
     5041 gccgtcctgc catgttctca agtatccact cccgtgcgaa ctcaccgctc tggatctcct
     5101 ggaggattgc cctcatctct tcccttgttt cctctgacac aacccttctt cccctggtca
     5161 tgtcaccgta ctccgccgtg tcgctgatgg agtatcgcat ataggagatt cctccctcat
     5221 acaggagatc gacaatgagc tttagctcgt gaagacactc aaaataggca atctccggct
     5281 gatatccagc ctctacgagc gtgtcaaagc cagccctcac aagttcagat gccccaccgc
     5341 acagaacagc ctgctcacca aaaagatctg tttcagtctc ttctgcaaag gttgtttcta
     5401 acaccccagc gcgggtggca ccaatgcctt tcgcataggc aagggctttc ttctttgcct
     5461 ttttagaata atcctgaaat acagcaatga gggaaggaac acctgctccg cgttcgaatt
     5521 cacggcgcac aaggtggccc ggtccttttg gagcaaccat aattacatcc acatcatcag
     5581 gaggtagaat ctggccgtaa tgaatattaa aaccgtgcga aaaaacaagg gtcttcccct
     5641 tcttaaggtt gtctttgatt tctgttttat agagctgggc ctggagatta tcctgggcca
     5701 gaatctgtat catgtcggcc ttttttgccg cctctgaagc ggataccggc ttaaacccgt
     5761 ggtcaacagc tattttatag ttgggtgtcc ccgtcagttc tgcaacaata acatcgaccc
     5821 cgctgtcccg aagattctgg gcctgcgcat gtccctgact tccgtaaccg attatagcaa
     5881 cttttgtccc tttaaggacc cccaggtctg catctttatc atagtacatc ttagccatct
     5941 tcctctcctc cttctaatgg tttatctttc agctttatga ctttttcgcc ccgtgctatg
     6001 gctatcggtc cggttctcac cagttccttg atgccaagag gcttgatgag ggtgaggaaa
     6061 gccctgagtt tttcatcatc cccagtaatg agcaaggtat aggtttttgg tgacacgtcg
     6121 ataatcttgc cgcggaatat ttctgtcatc cgaagaatct cagcgcgtgt gcgctcttct
     6181 gcgtttactt ttacgagcac catttctctc gaaacgtaat ccagttcctt gaaatcgacc
     6241 accttgatta cgtttatcag cttgttcagt tgcttcaaaa cctgctcaat gattgcttcg
     6301 ttgccgctgg tcacaatagt catggtcgat atggtggggt caagagtttc tgcaacacaa
     6361 aggctctcaa tattgaatcc tctgccactg aacaaacctg caacccgcga cagcacacca
     6421 aattcatttt caacaagaac agaaataata tgtctcaagt ctcgcctcct aaacgagaag
     6481 catctctttt aaagatgccc cggcaggaac catggggtaa acgctctcct cagggttaac
     6541 cttaacatca atcagagtcg gcctaccgtt atggaatgcc tcccgcaaaa ctgattcgac
     6601 ctcatcctca ctctctattc tgtatcctgc tgctccgtaa gcatccgcga gcttgacaaa
     6661 gtcaggggca cactcaaggc acgtccaggt ataccttttc tcatagaaga gctcctgcca
     6721 ttgccgcacc atgcccagat acccgttgtt cagaataact accttaacag gaaggttgaa
     6781 ctgaacagca gttccaagct cctgtatgtt catctgtatg cttccgtcac cggcaatatc
     6841 aacaacaagg ctcttgggga aagccacttg agctccaatt gcagcaggga atccaaaccc
     6901 catagtaccc agtccacctg atgtgagcaa tgttctgggt ttaagaaatt tgaagaactg
     6961 ggcagtccac atctggttct gcccgacctc ggtggcgata atggctttgc catcggtcaa
     7021 ttcaaagact ttctcaataa catactgggg ttttaatgtt ccgttcctga cgtatgtaag
     7081 cgggaaattt ttcttccact gctcagtctc attgagccag ggcccaatag atgccttata
     7141 cgccttgaat tcttccttct ctgcctcaac aatctcaacc atttttttga ggacgctcct
     7201 tgagtcgccc acgataggga tatcaacctt tatgttcttg ctgatggaag taggatcgat
     7261 atccacatga atgatctttg cgtggggagc aaactcatca accttgccgg tagccctgtc
     7321 gtcaaaccgg gccccaattg cgatgataag gtcagaactg gttatggcca tatttgcagc
     7381 gtatgttccg tgcataccca gcataccaag gaagagtttg tgacctcccg gaacccctcc
     7441 caaccccatg agtgtattaa ccacaggaat agacagttgt tccgcaaact taacgagttc
     7501 tgcagatgca ccggaagtaa tgattcctcc tcctgtatag agaacaggtt tttttgaagc
     7561 agcaatctgc tttattgctt tcttgatctg gcccaggtgc ccgttatatg ttggctggta
     7621 gcttcttata ttaaccttct cagggtattt gaaatcgcag attcctgaac taacatcctt
     7681 tggtatatcc accagaacag gccctggtct gcctgaacgg gcaatataaa atgcttcttt
     7741 gatgatccgc gcaagatcct ttacatcctt aacgaggtaa ctgtgcttgg tgcaaggcct
     7801 cgttatcccc acaatatctg cctcctggaa ggcatcattg ccaataagca tggtgtttac
     7861 ctgacacgta aagattacga tcgggattga atccatgtag gctgtggcaa ttcctgttac
     7921 tgtatttgtt gcacccgggc ctgacgtgac aagggagaca cccactttac ctgatgccct
     7981 ggcataacca tcagaggcgt ggaccgcccc ctgttcatga cgcaccacaa tttgatggat
     8041 ttcgctgtgg tcggtaagag catctgtaat attgagcgtg gcaccacccg gataacagaa
     8101 tatgttctca acaccctcaa ttttgagagc ctctacaaat atctgagcac ctgttttctt
     8161 caatccttct cccctttttt tggttcctcg ccaaccctct ccataatgtc cttaaagtaa
     8221 agcttttttt tctttaaaat tcttacttcc atctcttcat catctgtaag gtaaggcttt
     8281 ttcattaaca tctgcagcct tcggtcaagg gtggcgtgtt gagttttagc ccggttatag
     8341 atatcttctt tactttcgct ttgttccatg ggcaatggta gattaatcta taaaaattag
     8401 tgaaaaaagt caactattac caaaaaatgg tactacccga aaatcacaga acaaattatg
     8461 gtgcctcacc gtaaaaaaca aatcttatac aacattaaaa attagtaagg tttcggcaat
     8521 cttccggtta gcttgaactg tatatcctgt gacgtgagca aatcagtaaa gaggaggtct
     8581 aaccggaaat gtattggtct gggcacaaag gactgaaaaa cctcatatat ctcgtgttaa
     8641 ttgttgtgcc actcttctct tttggcctca ccaaccatgg gctctggtct gcagacgaac
     8701 caagggttgc cgagattgga agggagatgg cactcacagg aaactgggca gtgcctatgc
     8761 taaaccagag gccattcctt gaagagcctc ctctttatta cgcaactctg gctctgacct
     8821 tcaaagcact gggcgtgtct gacagagtcg caagaatacc ctcagcaatc tttgcgtttg
     8881 ccactgttct cgtcctgttc tttacggtca atttcctgtt tggaccgcag attgcactgt
     8941 tctccggaat catcctcgca accacagggg agtacttcag ggtagcccat acagtaatag
     9001 tcgatagcgc gctcactttt tttattgcct gtgccatgag tcttttcata gtgggatacc
     9061 tgtctgacag taacagaaaa aagttgctgt gctacatcct tctttatatc tcgtgcacct
     9121 tcgccttcta tacaaaagga ttcataggta ttgttattcc cgggcttggc attctttcat
     9181 ttcttgttgc cgacaaaaat ataaaagaaa tcatcaaaat gcggctttgg ctgggcattc
     9241 ttatctttct tgtcctgaca ctcccctggt tcatcaccct gtggcaacaa ggcggaaagg
     9301 aacatctgga cgtcttcctt ttgcacaatc acctccagag attcttgccg gggaagttct
     9361 ccggaaccat ttcaggagaa gcatcaggcc accatcaccc tttttactat tacctcattg
     9421 agttcccggt aggctttctt ccatggagtc tattactcgt tccagttttc atccatgcct
     9481 tttcgagatc agacaaatca gaaacaccac ccgagaaagg tcgtctcctt gcaaaatgct
     9541 ggttctttgc aggaatcgta ttcctcagcg ctgcttctac aaaacgaaca ctttacctga
     9601 tgcctatttt tgcaccaatg gcactgctca cttcattgta cgtggaatcg atgctttatc
     9661 gattggcaga caaaatcgaa aagacctttc tgtgggcctt tactgcgatc tttgccgtaa
     9721 tcggtatcag cgctgtacct gtctatagct ccatacaagg aatctatcca tggattcgtg
     9781 gtacaaacct gtttccttcg actgtgttgc tttcgttgct cattgtccca tcctctgttt
     9841 cagcaatgtg gtttctctgg agcaagaatt ttggaaaata ctgggttaca actatcatac
     9901 caattgttgt tggaatggtt ttcactgccg tggctgttgc acctgttgtc gatatccaca
     9961 agagctttgt ccccttctgt catgaactga tggctattgt accatccagc gaaccactct
    10021 atgcctatag cgcagatgag accctgcggg gcgctgtgcc attctacacc gggcgctacc
    10081 tcaccgagat ttgtggcccc ggaaatttgg aaaagactat cgagacaaaa ggacaggtct
    10141 ttatcattac cagggacaag aaaaggaagg ctgagaccga gctgctttcc acaggaaaat
    10201 tatcaatcat atttcagcaa aaaatgggtg gggagcgaac gctcacactc cttggaaaaa
    10261 acagccatac ggttcaaagc gatattcacc tcgctaagga caattgatag gatcggaatt
    10321 ggttttaaat tactactgca ccaggttttt tatcaggatc tactacatgg attttttcac
    10381 ctgcaccatc ttggtaacca gaaggtttat cgtgatggat ctcgaactat acggtctttg
    10441 cgaaagaata tgattgcctt gcctggagat cttcgcaagc gaaaagccat ataccttgtt
    10501 gtgatcattg ccgcttcata ccttttcttt ttctacaacc tcaatagcta ttctctgaag
    10561 gagcctgatg agggtcgata tgctgaaatc ccccgcgaga tggtagaaca ggggaattat
    10621 ctggtcccac acctcaacta cgtgcgctac tttgagaagc cacccctgct gtactggatc
    10681 acagcagcat cttataaagt gtttgggatc aatgagtggt ccttcagatt ccccaatgca
    10741 ctggctgcct ttttttgcag cattcttctt tacctcttct cttgtaaaag attttccccc
    10801 aagaccgggt tcattagttc tctaatcctc atttcctgtt tcggcttttt taccatggca
    10861 agaattgtca ccattgacat gctctttacc tgtttgctat tcggtgcact cctttccttt
    10921 catgaatatt atctgactca caagggcctt ttcttttatc tattccacat ctgccttagc
    10981 ctggctaccc ttgcaaaagg gccggcagct ttgatccttc tgggggttac catcctcata
    11041 tatctccgca cagaaaagcg cctctctttt ctgaaggacc tgctgagcta cagagccatt
    11101 cttatctttg cgattattac catgccctgg ttcgtcataa tgtcaatcaa ggaaaaagaa
    11161 tttttccatt tcttttttct tgatcagaac gtcttaaggt tccttaccac aaggcataac
    11221 cggagcggcc ccctgtatta cttcatacct gtcctcgcat taggcatgct gccatggtct
    11281 gtctttctcc ccagggtaat tatcaggctg tggtgggtca gagaactgcg tctctttttt
    11341 atatggtctg ccgttgtgtt tgcctttttc agcatctcag gctccaaact tcccccttac
    11401 atattacccg tcttccctgc ccttgcccaa atactgggtt atttctttgc cacccggcag
    11461 caagattcgg ttccagcaaa ctgtgaagtg attgcctatt tcgttttctt cgccactgtc
    11521 atgatggcag gattccttgg ggcttctgga atactgggaa aatacctgca agacgaactg
    11581 tatatacaag acattctgcc tgacatatgg gggcttgaac tcgggatagc cctggtttcc
    11641 gctgttatga tttgcttcat gtgcatcaga tctatgcgtc gtttcgggcc attcttttat
    11701 gcccttacca cattttcact ggtcattgtc atagcgctca tggtcaatac cagggtgatt
    11761 gataaacata cgactacaaa gggcctcgca aaaacaataa acaacttcca atccacacca
    11821 gcatacattg taaattatgg ttcattcgaa gagaccctcc cgttctatac caggcaaagg
    11881 attcatattg cctgttacaa aggcgaatta gccatggggt caaagtatcc tgatgcccgt
    11941 ccgttcttcc tcgaccaggc agcattcagg aaccttcttg attcaggtca gcctgttttc
    12001 gttgtcataa agaagaaata tctctccagt gctgcaaaaa caaccaatca agaactaaaa
    12061 ctgctggatt gccagaacga aagatgcctt gccgcaaacc aaaacgtaca ggtgttttat
    12121 ccggagctag ccttttatcg atgatccagg ggttggatct acaggtatat tcctgtttca
    12181 aataccacat aaaggtgact atgtaacatg atcgcaaagc cactccttct catgcatgcc
    12241 aaaaaaactg tgctattcca gcaatgtccc cacccaaatc tcttttctat cattcagaat
    12301 caaaagaact gaaaataaac aagagatcac tatgatggga aaacctatcc ttatcggtat
    12361 tgagctcccc cctctgcttg tttgaaccct tttataaaaa agaaggtact ggttcgggtg
    12421 cggctggttc atacactaaa caggttatca gtaaaaaaac agtaaagata atataccgtg
    12481 gacgttgata caaaaaagga ttattatgta gctattgttc agcgagagca agcctgtgtt
    12541 aaaatggact cacggaggta tacagtatgt tttattgcag attaatccct gtcatttgtt
    12601 tttttattgc tgttgctgtt tttagttttt cctcgccttt gcaaggggct gaatggaaga
    12661 tgttctatca aaaaatgaac gaaaagggcg caggaggaaa atatgaaaac ctcaactact
    12721 attacgacaa agaaagcatt gaaaaaccct ccaaaggcat tgcccgggta tggttcaaaa
    12781 caactgtagg ggatgatgac tcagagaatt tggctgaaaa agatggatca gacgaatcag
    12841 aacaatacag aggccatatt gaaatcaact gtaaaacaaa atcatataga atgatagagg
    12901 agacaagttc agattcttca gaatcagagg gaaaaggatt acaaccatca gccggtaagg
    12961 cccctcgccg actctatctc gattcagctt tgggtgcctt gtggtctaat ctttgcgaat
    13021 aatgaaaaca gggataattc ggtgaggaag gaaagagaca cacaaactca cccatcaaaa
    13081 tcgccagtat tcacaaattg gtatatagat attgtccaca attcttattt catgttcgag
    13141 gtatggttac cgt
//
LOCUS       DAOOMC010000082        12239 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdus sp. isolate AMR_MDS_2685
            NODE_5743_length_12239_cov_5.968894, whole genome shotgun sequence.
ACCESSION   DAOOMC010000082 DAOOMC010000000
VERSION     DAOOMC010000082.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864760
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdus sp. (wastewater metagenome)
  ORGANISM  Syntrophorhabdus sp.
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae; Syntrophorhabdus.
REFERENCE   1  (bases 1 to 12239)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12239)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,729
            Genes (coding)                    :: 2,708
            CDSs (with protein)               :: 2,708
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 7 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12239
                     /organism="Syntrophorhabdus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5743_length_12239_cov_5.968894"
                     /isolate="AMR_MDS_2685"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2699756"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..648)
                     /locus_tag="PK125_09170"
     CDS             complement(<1..648)
                     /locus_tag="PK125_09170"
                     /inference="COORDINATES: protein motif:HMM:NF015050.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB38315.1"
                     /translation="MRYVRKAPLKRQEIWKKFGVIPRGIDREVVEIMHRTHMGVDQDY
                     HHILLQTTRCALADGWGGSMISTDLQDIMFGTPVPVLGSINLGVLKDDEVNVIVHGHE
                     PLLPELLVVASKDPEIKALTEKVNAKGVNLAGMCCSANEVLMRHGIPCAGNFLQQELA
                     LVTGAVELMTVDVQCEMQGLQSVAECFHTRLITTSDRAKIEGATHVEFRPEHGLDT"
     gene            complement(645..1133)
                     /locus_tag="PK125_09175"
     CDS             complement(645..1133)
                     /locus_tag="PK125_09175"
                     /inference="COORDINATES: protein motif:HMM:NF015050.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB38316.1"
                     /translation="MTDETLKSIDKATTELLEKAKKDEIGTVFSRAEQIKPCPVGLEE
                     SCCKICAMGPCRLPRTKKALQKERVGVCGATIDTIVARNFARRVAAGVASHSDHAREV
                     AETFIKTARGESQGFSIKDEIKLMEVALDFGVEIENKEVKEIALDLGGRVLENLGDRM
                     VS"
     gene            complement(1134..2084)
                     /locus_tag="PK125_09180"
     CDS             complement(1134..2084)
                     /locus_tag="PK125_09180"
                     /inference="COORDINATES: protein motif:HMM:NF003377.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA decarbonylase/synthase complex
                     subunit delta"
                     /protein_id="HPB38317.1"
                     /translation="MFEISKVKYTGKIKEVTLGSGEKAVIIGGETCYPFHLFEGEMPN
                     APKIAFEVWDFEPDDWQDWAIEPFRDVIGNPAAWARKCVEVFHAEIIALNLKGADPNG
                     KDRVVEEVVSVVKNVIEAVDVPVIVWGTSNDEKDAVLLRRVAEVCEGKNVAIGPLSEK
                     NYKHIGATAIAYNHPVLASSPIDVNLAKQLNILLGNLGVKDEKILIDPTTGGLGYGLE
                     YSYSVIERDKMAGLTQEDIKLQYPIVCNLAQEIWKTKEAKLTQEDDPKLGDEKKRTIL
                     LESVSAMSMLIAGADVVIMRHPESIALVQEMIKELTSRGI"
     gene            complement(2163..2378)
                     /locus_tag="PK125_09185"
     CDS             complement(2163..2378)
                     /locus_tag="PK125_09185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB38318.1"
                     /translation="MGDYVYEEKKNYEGKNVKVLGSTFEEGKPDATGNWLAKLTTHEE
                     RLGYLRTALRYWYSNEWYGSEKRKQEA"
     gene            complement(2524..3288)
                     /locus_tag="PK125_09190"
     CDS             complement(2524..3288)
                     /locus_tag="PK125_09190"
                     /inference="COORDINATES: protein motif:HMM:NF013793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HPB38319.1"
                     /translation="MPIIIGFAGKGGVGKTTIAGILIRYLIEELKVAPVLAVDADPNS
                     NLNEMLGVNVHTTIGEARELLKKDVPTGMTKDVWFEYKVHEAIIEGKGFDLLVMGRPE
                     GPGCYCAANALAKRSIESLKTNYPFVVADNEAGMEHLSRLVTQDLDHLYVISDPSPRG
                     LLTGKRILELVKELRLNIKNWHIIVNMARESEEEKVRDLAVNRGFEVTGFVREDKILE
                     KADTEGVSIFSISRESSAISDSYSIFAKTINAVHVK"
     gene            complement(3310..5253)
                     /locus_tag="PK125_09195"
     CDS             complement(3310..5253)
                     /locus_tag="PK125_09195"
                     /inference="COORDINATES: protein motif:HMM:NF025925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASKHA domain-containing protein"
                     /protein_id="HPB38320.1"
                     /translation="MNKYRITFLPSERIFDAHEGDTILDAAMKAGVYINASCGGNSSC
                     GKCRIKVIQGSVISTRHPKISKADYDKGYRLACRSRPESNLVVEVPIESQIDGSILKD
                     NGRKPLILLVSDLGMLIKQRKADPPFFKLYIELPEPTAEDNISDFERLVRELGRILKK
                     HIFSADLNVLKSMARTFRESIWKITVTLTLCNNGFRIVNVETGKKMLENYAIVLDIGT
                     TTVSGQIIDLTNYGHDICTLAEASDYNGQIRFGDDVISRIMYCRKTGGLANMQKALGD
                     TVNGIISQLVKKSGVRKASISHLIFAGNTTMTHLFFGLDPENIMLAPYTPVTNVYPQA
                     IAKDFGLDLEGHVYVIASPCVSSYVGGDVVAGVVGCGMSRSEKVTLYIDVGTNGEIVL
                     GNKDWLICASCSAGPAFEGGGIQFGMRAETGAIEQLKINPVNYEPMIITIGKAKPKGI
                     CGSGLIDGISELLLTGILDQNGKFKRDLDDSRVREGESGFEYVVASKENTQICKDIVL
                     TEVDLANVIRAKAALYAGCKVLVESIGLTFHDIERVIIAGGFGHYIDLEKAQIIGLLP
                     ELPLERFIFVGNGSLLGTRLFSFSRRFIDEAQSISKKMTNVELSTSQKFMDEFVAASF
                     LPHTDDKAFPGVLKKLHRYKLLP"
     gene            complement(5841..6731)
                     /locus_tag="PK125_09200"
     CDS             complement(5841..6731)
                     /locus_tag="PK125_09200"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021616.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HPB38321.1"
                     /translation="MVSIVITTYNRRAFLKEAVLSVINQDYKNKEVIVVDDGSFDGSS
                     EEVRHLPLRYVWKQNRGISSARNMGIQVSKGEYIAFLDVDDLWKKGKLTQQMSIMADN
                     DADISYTDEIWIRNGEHLNQKNRHKKYSGFIFERCLPLCIISPSSVVIRRKLFDEVGL
                     FDESLPVCEDYDMWLRITCRYPVLFIDKQFIIKRGGHKDQLSRAYRAMDRFRIQSLVK
                     IIQSSTLNPEMRKVALNELKRKCIIYAMGAKKRGRFQESNAYMQLYETLANQPLVSGL
                     KDRVIQGLAGSKRPIGLEKV"
     gene            complement(6700..8769)
                     /locus_tag="PK125_09205"
     CDS             complement(6700..8769)
                     /locus_tag="PK125_09205"
                     /inference="COORDINATES: protein motif:HMM:NF020660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1926 domain-containing protein"
                     /protein_id="HPB38322.1"
                     /translation="MNLIICIHNHQPVGNMDYILEDAYKAAYLPFFEVLKEYKDIKIN
                     LHFSGYLFTWLLEKKPDYVALLKLLKEQGRIEVVSGGMYEPVLSLIPEDDGCNQIRMH
                     ADLMEETFGERPKGAWLAERVYEPHIPQTLSKAGITYTLVDDNHFKAIGMTEDDLFGY
                     YMSEFEGHKLAIFPGLEFLRYTIPFKPLDVLDTYLKDADGKGHKLAVFGDDGEKFGLW
                     PGTKKSVYEEGWLKMFFEYLTGNQKWLRTVTFSEYLATPPKGPIYLDCQSYKEMGEWS
                     LPPIFAKEYSAAISDKKPSKKTIIKGGYFKYFLIKYPESNDMHKKMLSLTKKANENPE
                     AKKHVYMAQCNDSYWHGVFGGLYLPHLRASVYEHLIEAEKILDPKEPFVTGIIDDVNM
                     DGIDEAVLTNNFLKAYFYLKEGGTLYELDYKPASSNIMANLYRRYEGYHDKVKMTSST
                     DVADGTKTIHDMFMAKEEGLERFLHYDWYRRASLVDHVMGKDVTWESFYRCQYFEPGD
                     FVKEPYEASCKNENKSTILSLVREGHYWKGGIGIPLVIKKTIRMTANESAVYIDYQID
                     GDVKENFLFGVEFNFSFLGSGGDRYMDLGSGRIPLTSKGIYKPSRMLQFYDPYQKVAP
                     TIECDHPISVWTFPVEVVSLSETGFERNYQSTMCMPVWDIDLTAGSRAIHLKLTVNGI
                     NRNNDLQ"
     gene            complement(8787..8864)
                     /locus_tag="PK125_09210"
     tRNA            complement(8787..8864)
                     /locus_tag="PK125_09210"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8827..8829),aa:Asp,seq:gtc)
     gene            complement(8872..8946)
                     /locus_tag="PK125_09215"
     tRNA            complement(8872..8946)
                     /locus_tag="PK125_09215"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8912..8914),aa:Val,seq:tac)
     gene            complement(8980..10239)
                     /gene="clpX"
                     /locus_tag="PK125_09220"
     CDS             complement(8980..10239)
                     /gene="clpX"
                     /locus_tag="PK125_09220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HPB38323.1"
                     /translation="MTMIKKSNGRTMKLHCSFCGKSQDEVKKLIAGPMVYICDECIEL
                     CNEIIQDEAKKERFDYIKTSIPKPHEIRRLLDEYVIGQDFAKKVLSVAVYNHYKRIES
                     KATFDDVEIQKSNILLIGPTGSGKTLLAQTLAKILHVPFTIADATTLTEAGYVGEDVE
                     NIILYLLQSADYNVERAERGIVYIDEIDKISKKIDSPSITRDVSGEGVQQALLKIIEG
                     TVANVPPKGGRKHPQQDYIKVDTSNILFICGGAFHGAESIVANRIGKKSIGFGVDVEG
                     GRDKQYSELIKDIQPEDLLKYGLIPEFIGRLPIIATLGELTEDNLVEILKRPRNAIVK
                     QYKRLFDLENVKLTFTEGALRGIAREAMKKKTGARGLRSIMERVMLDVMYEIPALTNV
                     KECVLSEEVIVNHDRPILIYEEEAEIA"
     gene            complement(10236..10820)
                     /gene="clpP"
                     /locus_tag="PK125_09225"
     CDS             complement(10236..10820)
                     /gene="clpP"
                     /locus_tag="PK125_09225"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010919829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HPB38324.1"
                     /translation="MNLVPIVIERDGRGERAYDIYSRLLKERIVFLGTAIDDNVANII
                     IAQLLFLESEDPTQEIYVYINSPGGYITSGLAIYDTMQYVKAPIVTMCIGQAASMGAV
                     LLAGGTQGKRYALPHSRILIHQPLGGAQGQATDIDIQAREILRIREEINKIFTKHTGQ
                     PIEKISADTERDFYMTAEQALNYGIIDKIVEKRQ"
     gene            complement(10817..12094)
                     /gene="tig"
                     /locus_tag="PK125_09230"
     CDS             complement(10817..12094)
                     /gene="tig"
                     /locus_tag="PK125_09230"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HPB38325.1"
                     /translation="MNVDVESLDKVRKKVQVLLPEEQLLELRESIYEELRKKAKIKGF
                     RPGKVPKSIITTYYKDYIEEELQTRIVQSTMGEALTTANVEPVSEPIVNFIEEEGRHG
                     YELECEVIPDIELPHYKGVEVEVEAINVTDEDVEKRIDGLQHMHAEMIAREGDAVTQK
                     GDFIIIKYQGYMNGKPAKGVGAEFYPVELGSTTLMPEFEEGLIGLRVGEEKEIEITFP
                     EDYPDKEIATKTMQFQVLVKEVKEKRLPEINDEFAKDLNFENIEAMKIGLREELKKEK
                     ETAQKREISQKIMEILLESIEIPVPKRLLDRRIQGMIEDAKSRFKTDNIPKEEEVNLD
                     TNLRRDFEPRAKERIKGEIIIKKIADVESITVNDDEIHEKMKKMAEETRRSYEEIEKF
                     YREYNMVESLRANILEEKTLAFLRENAVVKEKQ"
BASE COUNT         3234 a         2937 c         2165 g         3903 t
ORIGIN      
        1 agtgtcgagg ccatgctcgg ggcgaaactc gacatgggtt gcgccctcta tcttcgccct
       61 gtctgaggtg gtaatgagcc tggtgtgaaa acattcagct acactctgca acccttgcat
      121 ctcacattgc acatctacag tcatcaactc tactgcgccg gtaacaagag caagttcctg
      181 ttggagaaaa ttaccagcac agggaatgcc atgcctcatg agtacttcgt tcgccgaaca
      241 acacataccg gcaagattca ctccctttgc atttactttc tcagtaagcg ctttgatttc
      301 tggatcttta cttgcaacaa caagtaattc aggaagcaaa ggttcatggc catggacaat
      361 aacattcacc tcatcgtcct ttaatacccc aaggtttatg cttccaagga ctggtacagg
      421 ggtaccgaac attatatcct gcagatccgt cgaaatcata gaaccacccc atccgtctgc
      481 aagggcacat cgtgttgtct ggaggagaat atgatggtaa tcctgatcta cccccatatg
      541 agtgcgatgc atgatctcta caacttctct gtcaataccc cgtggaataa caccgaattt
      601 cttccatatc tcctgcctct tgagtggtgc ttttctaaca tacctcagct caccattctg
      661 tctcccaaat tctccagaac cctaccgccc aaatctaaag caatctcttt tacctcttta
      721 ttttctattt caactccaaa atctagggcc acctccataa gttttatttc atctttgatt
      781 gaaaatcctt gtgattcacc tctcgcagtt tttataaatg tttcggcaac ctccctggca
      841 tgatcagagt gagacgcaac gcctgcagca accctcctcg caaaatttct agccacaatt
      901 gtatcaatcg ttgctccgca gactcctact ctttccttct gcaaggcttt ctttgtcctg
      961 ggtagcctgc aaggccccat tgcacaaatc ttacagcaac tctcttccaa ccccacagga
     1021 cacggtttta tctgttcagc gcgagagaaa actgtaccta tctcgtcctt ttttgccttt
     1081 tcaaggagtt cagtcgttgc cttgtcaatg ctttttagtg tttcatctgt catctatatc
     1141 cctcttgatg tcagttcctt gatcatctct tgtacaagtg caatgctttc aggatgtcgc
     1201 atgataacaa catcggctcc agcaattaac atgctcatgg cactaaccga ttccagaaga
     1261 atggttcttt ttttctcatc accaagtttt gggtcatcct cctgtgtcaa tttcgcctct
     1321 tttgttttcc atatttcttg tgccagatta catacaatgg ggtactgaag ttttatgtct
     1381 tcctgtgtca accctgccat cttatctctt tcaatcacag aatacgagta ttccagccca
     1441 tagccaaggc caccggttgt tgggtcgatc aatatctttt catctttgac tccaaggtta
     1501 cctagaagaa tgttcaactg ttttgcaaga tttacatcaa tgggggatga agctagcaca
     1561 gggtgattat acgcaattgc cgttgcccca atgtgcttat agtttttttc cgacaaaggg
     1621 ccgatggcca catttttacc ctcacacacc tcagctactc ttcttaggag aacagcgtct
     1681 ttctcgtcat ttgacgtacc ccatacaata acaggcacat cgactgcttc aataacattt
     1741 ttgactacgc taacgacctc ctcaacaacc ctatcctttc cattcgggtc agcacctttc
     1801 aaatttaagg caataatctc tgcatggaag acttctacac attttcttgc ccatgctgca
     1861 gggttgccta taacatctct aaagggctct attgcccaat cttgccaatc atcgggttcg
     1921 aaatcccaga cttcgaaggc aatttttggt gcattaggca tctctccttc aaaaagatga
     1981 aaagggtaac atgtttctcc tccaatgatt acagcttttt cacccgatcc caaggtaact
     2041 tctttaatct tgcctgtata ttttaccttt gaaatctcaa acactttttc tcctcccttt
     2101 actaatcaca gagtaactaa tccatcctct gcttactgct caccattcac tacttatcct
     2161 ctttatgctt cctgttttcg tttctcactg ccgtaccatt cgttactgta ccagtaacgc
     2221 agggcggttc tcagataacc tagcctctct tcatgtgtcg tcagtttggc tagccagttg
     2281 cccgtagcgt caggcttacc ttcttcgaaa gttgatccca gcacctttac attttttcct
     2341 tcatagttct ttttttcttc ataaacataa tcacccatac aagtctccaa atcaaagaaa
     2401 cttcataact cttttaatat accacctgat gatggaacag ataaagtcct tttgggcttt
     2461 tgggataaat agccaagtgt tcacctcaaa cgattgcgtt ggcagtattc aggaactatg
     2521 gtttcattta acgtgtacag cgttaatagt tttggcaaaa attgaataag aatcagaaat
     2581 tgctgacgat tccctagata tcgagaaaat acttacccct tcagtatcag ctttttcaag
     2641 aatcttgtcc tcccttacaa acccagttac ctcaaaccct ctgttgactg ctaaatcacg
     2701 aactttttct tcttcagatt ctctggccat gtttacgatg atgtgccaat ttttaatgtt
     2761 aagcctcaac tccttgacta attcaaggat ccttttcccg gtgagcaaac cccttgggga
     2821 tgggtcagaa atcacataaa ggtgatcgag atcctgagtg acaagcctgc tcaagtgttc
     2881 cattcctgcc tcgttatcag ccactacaaa aggataattc gttttgaggg attcaatgga
     2941 tcgcttcgca agtgcgtttg cagcacaata acaaccaggt ccttcaggtc tacccatgac
     3001 aagcaggtca aagccctttc cctcaatgat ggcttcgtga actttatact caaaccaaac
     3061 atcctttgtc attcccgtgg gtacatcttt tttcagcaat tctcgtgctt caccaatagt
     3121 agtgtgaaca ttaacgccga gcatctcgtt caggtttgaa ttcgggtcag cgtccactgc
     3181 gaggacaggt gctactttta actcttctat gagataacga attagtattc ctgctattgt
     3241 tgtttttcca acaccacctt tgcctgcaaa accaataatt atgggcattg aaaactctcc
     3301 cttcatcttt catggtaaca gcttgtatct atgaagtttt ttcaatactc caggaaacgc
     3361 tttatcatcg gtatgaggca aaaaggatgc tgccacaaat tcatccatga atttttgact
     3421 ggttgagagt tctacattcg tcatcttttt tgatatgctt tgtgcctcgt ctataaatct
     3481 cctggagaac gaaaaaagcc ttgttccgag aagtgatcca ttgcctacaa aaataaaacg
     3541 ttcaagaggc agttccggca aaagcccgat aatttgagcc ttttcaaggt caatataatg
     3601 gccaaaacca cctgcaataa taactctctc aatatcatga aaggttaggc cgatactttc
     3661 gacaagcacc ttgcaacccg catagagtgc agcttttgca cgaattacat tggcaagatc
     3721 aacttcggta agtacaatat ctttacaaat ttgcgtgttt tcttttgatg ccacaacata
     3781 ctcaaatccg ctttcgcctt ctcgtacacg tgaatcatcc agatctcttt tgaacttacc
     3841 gttctgatca agaatccctg ttaataaaag ttctgaaata ccgtctataa ggccagaacc
     3901 acatatacct tttggcttgg ccttgccaat cgttatgatc atgggttcgt aatttaccgg
     3961 attgatcttc agctgttcta tggcacctgt ttcggcccgc ataccaaact gaattccacc
     4021 accctcaaac gcaggacctg ccgaacatga agcacagatg agccagtctt tgtttccaag
     4081 aacaatctcg ccatttgtcc ctacatctat gtaaagcgta accttttcgc ttcgggacat
     4141 gccacaccct acaacaccag ccacaacatc tccaccaacg tatgaagata cgcatggtga
     4201 agctataaca tatacatgac cttccaaatc cagtccaaaa tccttagcga ttgcttgagg
     4261 ataaacattt gttacaggtg tatagggtgc aagcattatg ttttcagggt caagaccaaa
     4321 aaaaagatgc gtcattgtcg tatttcctgc aaatatgagg tgagaaattg aggccttccg
     4381 aacgccactc tttttaacaa gttggctgat aattccattt acagtatcgc caagtgcttt
     4441 ctgcatattg gcaagaccgc ctgttttccg gcaatacata attcttgata taacatcgtc
     4501 tccaaatctt atctgcccat tgtaatcaga tgcctcagca agggtacaaa tatcgtggcc
     4561 gtaatttgtc aggtcaatga tttgtccgga aaccgtggta gttcctatgt caagtacaat
     4621 tgcgtaattc tcgagcatct tttttccggt ctcaacattt acaatccgaa atccattatt
     4681 acacagagtc agtgtgacag tgattttcca tatggattca cggaatgttc gggccatact
     4741 ttttaaaaca ttaaggtcag cagaaaatat atgtttcttc aatatcctcc caagttccct
     4801 gaccaatctc tcaaaatctg aaatattgtc ttctgcagta ggttcaggaa gttctatata
     4861 taatttgaaa aaaggtggat ccgctttccg ttgttttata agcattccta ggtctgaaac
     4921 cagcagaata agtggttttc tgccattgtc tttcagtatg cttccatcaa tttgtgactc
     4981 tatgggtact tctacgacta gattgctttc cggccttgat ctgcaggcaa gacgataacc
     5041 tttatcatag tcagcctttg aaatctttgg atggcgcgtc gaaatgacac tcccttgaat
     5101 tactttaatc ctgcatttac cgcatgagct attccctccg cacgatgcat ttatgtaaac
     5161 accagccttc attgctgcat caagaatagt atcaccttca tgtgcatcaa atatgcgttc
     5221 ggacggtaaa aatgttatac gatacttatt catcaagtga gatattccaa tatttcgtag
     5281 gccattgtca agtatagtga agggtcacta ctctaaagta tactgatagt tcttcgatat
     5341 tattataaat ggcaacaggc taagattaaa aaattagtaa agtgtggatt gcataatcaa
     5401 tgaggttatt tttagcaaaa aagcagagga agtggggttc aagacgcttg acgcgttgag
     5461 gggactacgt gagttttagc ccctgaagtc gtgacaagaa gcattgacat cattatgaaa
     5521 gagtaataga cttgttacgt atcatcatcc aaatcacagg aggattttat gaaacgtttt
     5581 tttctgttca gtgttatagc aatttttacg attacttttt ttgtagtacc ggtcttttcg
     5641 caggatgttt acaaaacatg gcaaccacca aatgctgaac cggaaggcga aaaagtttta
     5701 tgggatgctc ttgttatgcg acctgcagga ctgattacct gtgtattagg aatcggtggt
     5761 tatattgtgt ctctgccgtt ttcactgact tcacaaaccc agaatcaagc ctttcatagc
     5821 ttgatagtgg aacctataaa ctatactttc tcaaggccaa taggcctttt ggaaccagct
     5881 aaaccctgaa tgacccgatc ctttaagcca gatactaggg gttgattcgc aagggtttcg
     5941 tagagttgca tataagcatt tgactcttgg aatctacccc ttttttttgc ccccatcgcg
     6001 taaattatgc attttctttt taattcgttg agcgctacct tgcgcatttc tgggtttaaa
     6061 gtactgcttt gtattatttt aacaagactc tggattcgaa atctgtccat agcccgataa
     6121 gctcttgata gttgatcttt gtggccacct ctttttatta taaattgctt gtctataaat
     6181 aagactgggt acctgcatgt tattctgagc cacatatcgt aatcttcaca tacgggcaga
     6241 ctttcatcaa acaacccaac ctcatcgaaa agtttccttc ttattactac tgaggatggg
     6301 cttatgatac agagaggaag gcaccgctca aaaataaagc cagagtactt tttgtgtctg
     6361 tttttttggt ttaagtgctc accatttctg atccagattt catctgtata cgagatgtca
     6421 gcgtcattgt ctgccattat actcatttgc tgggtgagct tacctttttt ccaaagatca
     6481 tctacatcaa gaaaagcaat gtactccccc ttagagacct ggatgcccat atttcgtgcg
     6541 ctgcttattc ctctgttttg cttccaaacg taacggaggg gaagatgcct tacctcttcc
     6601 gaggatccat caaaagatcc atcatcaacc actattactt ctttgttttt ataatcctga
     6661 ttaattacgg agagtactgc ttctttgaga aatgcccttc tattgtaagt cgttattacg
     6721 attgatacca tttactgtaa gcttcaaatg aatagctctt gaaccggcag ttaaatcaat
     6781 atcccacact ggcatgcaca tggtactctg gtagttccgc tcaaaccctg tttcagagag
     6841 agaaacaacc tctaccggga aagtccagac tgagataggg tggtcacact cgatggtcgg
     6901 tgccactttt tggtatgggt cataaaattg taacattcgt gacggcttat atatgccttt
     6961 tgaagtcaag ggtattctgc ctgaccccag gtccatatat ctatcaccgc cacttccaag
     7021 gaaagagaaa ttaaattcca caccaaaaag aaaattctct ttcacatcac catcaatttg
     7081 ataatcaatg tacactgctg attcattggc ggtcatgcga attgtttttt taatgacaag
     7141 aggaattcct attcctcctt tccagtaatg accttctctc acaagagaaa gaattgtgga
     7201 cttgttttca ttcttacagg acgcctcgta tggttcctta acaaaatctc cgggttcaaa
     7261 atactgacat ctgtaaaagg actcccaggt aacatccttg cccataacat gatcaacaag
     7321 tgaagcacgc cggtaccagt cgtagtgaag gaaacgctcc aggccctctt cctttgccat
     7381 aaacatatca tgaattgttt tggtgccatc tgccacgtca gttgaagaag tcattttgac
     7441 cttatcatgg tatccttcat atcgcctgta aaggttagcc attatatttg aagaggccgg
     7501 tttataatca agttcataca atgtgccacc ttcttttagg taaaagtaag ccttcaaaaa
     7561 attattagta agaacggctt catctatacc gtccatgtta acatcgtcaa ttatcccagt
     7621 aacaaagggt tcttttgggt ccaagatctt ttcggcttca ataagatgtt cgtagacgga
     7681 cgcccgtaga tggggcagat agagcccgcc aaacactcca tgccaataac tatcgttgca
     7741 ctgagccatg tacacatgct tctttgcttc agggttttca ttcgctttct ttgtcaatga
     7801 aagcattttt ttgtgcatgt cattactttc agggtatttt atcagaaaat atttgaagta
     7861 cccaccttta ataattgtct tcttactggg ttttttatca gaaattgcag cactatattc
     7921 tttcgcaaaa attgggggca aactccattc tcccatttct ttataggact gacaatcaag
     7981 gtagataggg cctttcgggg gggtcgcaag gtactcagag aacgtaaccg ttctaagcca
     8041 cttctgattc ccagttaaat attcaaagaa cattttcaac catccctctt catagactga
     8101 cttcttcgtc cccggccaaa ggccgaactt ttcaccatca tcaccgaaaa cagcaagctt
     8161 gtggcctttc ccatctgcgt cttttagata agtgtccaaa acatccaatg gtttaaaggg
     8221 tattgtatag cgcagaaatt caaggccagg gaaaatagcc aatttatgtc cttcgaattc
     8281 gctcatataa tatccgaaca ggtcatcttc ggtcataccg attgctttaa aatgattgtc
     8341 atccaccaag gtatatgtga tccctgcctt gcttaaagtt tgcggtatat gtggttcgta
     8401 aacccgttca gcaagccatg cgccctttgg cctctcaccg aaggtttctt ccataaggtc
     8461 tgcatgcatc ctgatctgat tacacccatc atcttccgga ataagtgaaa gcaccggttc
     8521 atacatacca cctgaaacaa cctcgatccg tccttgttcc ttcagtaact ttaggagagc
     8581 aacgtaatca ggtttctttt ccagaagcca tgtaaaaaga taaccagaga aatggagatt
     8641 gatcttgatg tctttatatt ctttgagtac ttcaaaaaaa ggcaaatatg cagctttata
     8701 agcatcttca aggatataat ccatgtttcc cacgggttga tgattatgaa tacagataat
     8761 gaggttcatg tagaccttta aagaaatggc ggggtcgacg agcttcgaac tcgcggcctc
     8821 cggcgtgaca ggccggcgtt ataaccaggc ttaactacga ccccgcatga atggtaggcg
     8881 ggagaggttt tgaacctccg acccccggct tgtaaggccg atgctctccc actgagctac
     8941 ccgcccatac tatatcagct taccactcct gtgtcaacct tatgcaattt cggcctcttc
     9001 ttcatagatc aaaattggcc tgtcgtgatt cacaatgact tcttcactca atacacactc
     9061 tttgacattt gtaagggccg gaatttcgta cataacatcg agcataaccc tttccatgat
     9121 tgatctcagc cctcttgcac cagttttctt cttcatcgcc tcccgtgcaa tacctcttag
     9181 ggctccttcc gtaaacgtta atttaacgtt ttccaggtca aaaagtcgct tgtactgttt
     9241 tacaatggca ttgcgtggtc ttttgaggat ttcaacaaga ttgtcctcag tcaattctcc
     9301 gagcgtggca ataataggta atcttcctat gaactcagga attaagccat atttgagaag
     9361 atcctcaggc tgaatatctt ttatcaattc tgaatattgt ttgtctcttc caccctctac
     9421 atcaacacca aaaccgatgc ttttctttcc aatcctgttc gcaacgatag actctgctcc
     9481 atgaaatgcc cctccgcaaa tgaaaaggat gtttgacgta tctaccttta tataatcctg
     9541 ttgtggatgc tttcttccgc cctttggagg tacattagcc acagtgccct cgattatctt
     9601 caatagggcc tgctggactc cctcaccgga aacatcacgt gtaatggaag ggctatctat
     9661 tttttttgaa attttatcta tttcatcaat ataaacgatg ccacgttctg cccgctcaac
     9721 attataatca gcagactgga gaagatagag gattatgttc tccacatcct cgcccacata
     9781 ccctgcctca gtcaaagttg tagcatccgc tatagtaaag gggacatgca ggattttcgc
     9841 caatgtctga gcaagcagtg tcttcccaga tcctgtggga ccaataagga gaatattgct
     9901 tttttgaatt tccacatcat cgaatgtggc ttttgattca attctcttat aatgattata
     9961 aactgccact gaaaggactt tttttgcaaa gtcttgacct atgacatact catcaagaag
    10021 tcttcttatt tcatgaggct ttggaatcga tgttttaata taatcaaatc tttccttttt
    10081 tgcctcatcc tgaataatct cattgcaaag ttcaatacat tcatcacaga tgtataccat
    10141 cggtccagca ataagttttt tgacctcatc ttggcttttt ccacaaaaag aacaatggag
    10201 tttcattgtc cttccgttac tcttcttaat cattgtcatt gtcttttctc cacgattttg
    10261 tcaatgatgc cataatttaa ggcctgttca gctgtcatat aaaaatctct ttctgtatcg
    10321 gcagagatct tttcaattgg ctgacctgtg tgtttcgtaa agatcttatt aatctcttct
    10381 cttattcgta gaatttcccg tgcctgaata tcaatgtcag ttgcctgacc ctgagcccca
    10441 ccaaggggtt gatggatcaa gattcttgag tgtggaagag cataacgctt gccctgagtc
    10501 cctccagcca gcaggactgc acccatgctt gctgcctgtc cgatgcacat tgttactatg
    10561 ggcgccttaa catattgcat ggtgtcgtaa atggcaagcc cgcttgttat ataacctcct
    10621 ggggagttta tataaacata gatctcttgc gtagggtctt cagattctag gaacagaagt
    10681 tgggcaataa taatattcgc cacattgtca tctatagcag ttcccaaaaa aactatcctc
    10741 tctttcagaa gacgtgaata tatatcatat gccctctcac ctcttccatc tctttctata
    10801 actatgggaa ccaggttcat tgtttctcct ttactactgc gttctcccgg agaaaagcaa
    10861 gtgttttttc ctccaaaata tttgcacgga ggctctctac catattgtat tctctataga
    10921 atttctctat ctcttcataa cttcttctgg tttcctccgc cattttcttc atcttctcat
    10981 ggatttcatc atcattaaca gtaatcgatt caacatcagc aatctttttg ataataattt
    11041 cccctttgat ccgttctttg gccctcggct caaaatccct tctcaagttc gtatcaagat
    11101 ttacctcttc ttccttaggt atattatcag ttttaaatcg actttttgca tcttcaatca
    11161 tcccttgtat ccttctatcg agaagtctct taggtacagg gatttctatg ctttcaagaa
    11221 gaatctccat aattttttgc gaaatttctc ttttttgagc tgtttccttc tcttttttca
    11281 gctcttcccg aaggccaatc ttcattgcct caatattttc aaaattgagg tctttagcaa
    11341 actcatcgtt tatctccggt aatctctttt ctttaacttc cttgacaaga acttgaaact
    11401 gcattgtttt cgtagctatt tctttgtctg ggtaatcttc aggaaaggtt atttcaattt
    11461 ctttctcttc ccccaccctc aagccaatca ggccttcctc aaactctggc ataagcgttg
    11521 ttgatcccaa ttcaacagga tagaactcag caccaacacc ttttgcaggc ttgccattca
    11581 tgtatccctg gtactttatt atgataaagt cacccttttg ggtaacagca tcgccctctc
    11641 tggcaatcat ctctgcatgc atgtgctgca gaccatctat tcttttttcg acatcctcat
    11701 cggtaacatt aattgcttcg acttctacct ccaccccttt atagtgaggc aactcgatgt
    11761 cgggaatcac ctcgcattct agctcataac cgtgccggcc ttcctcctcg ataaaattga
    11821 caataggctc actcacaggc tcaacatttg ccgtggttag agcttctccc atggtagact
    11881 gcactattct ggtctgtaat tcttcctcaa tatagtcctt ataatatgta gttataatcg
    11941 attttggaac ttttcctggc ctgaagcctt ttattttggc cttttttctc agttcttcgt
    12001 aaattgattc acgaagttcc aatagctgtt cttctggaag aagcacctgt actttcttcc
    12061 taaccttatc aagagactct acgtcaacat tcattcatca ccccggaaaa ctcaatctgg
    12121 tacagaccat actggacttc agacaactat tatacaccta acttcttaaa ttatcaagct
    12181 atgggatttg ttgccttttg cacgtgcaca gcgtatgata tcctacaatg atgatgtgt
//
LOCUS       DAOOMC010000083        12106 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdus sp. isolate AMR_MDS_2685
            NODE_5839_length_12106_cov_5.850801, whole genome shotgun sequence.
ACCESSION   DAOOMC010000083 DAOOMC010000000
VERSION     DAOOMC010000083.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864760
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdus sp. (wastewater metagenome)
  ORGANISM  Syntrophorhabdus sp.
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae; Syntrophorhabdus.
REFERENCE   1  (bases 1 to 12106)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12106)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,729
            Genes (coding)                    :: 2,708
            CDSs (with protein)               :: 2,708
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 7 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12106
                     /organism="Syntrophorhabdus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5839_length_12106_cov_5.850801"
                     /isolate="AMR_MDS_2685"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2699756"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(107..1147)
                     /locus_tag="PK125_09235"
     CDS             complement(107..1147)
                     /locus_tag="PK125_09235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB38326.1"
                     /translation="MMSSRIRFQVFQSESTFLHPRTGFSPVTERFEVRIDPLTGRTGH
                     FSHFGAIKPQKLSLDAYATPEIKGFCPFCLEHRDRVTPRFKDDILSEGRLIRNEACLI
                     PNLFPYDVHSGVLIMTDHHVVHLKDLNEQRLFDAFWLGIHFLQRIKAISPEQPYHLMT
                     WNYMPPSGGGLVHPHQQYFATSNPGNQFSDELKASNAFYNSYGTNYWREFIEVERLQD
                     KRFIGQIGSSYWLSSFVSMGLLGEIMCVFPEVFDVSQFAETHMEDLVAGLLKIFKYYI
                     STDIYSFNTSLFFGPEGQQSFACHFRIAARTFLNTRDYAPDLNFFQVLLSEPVSVVRP
                     EELAADLKEFFR"
     gene            1342..1416
                     /locus_tag="PK125_09240"
     tRNA            1342..1416
                     /locus_tag="PK125_09240"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1374..1376,aa:Gly,seq:gcc)
     gene            1525..1599
                     /locus_tag="PK125_09245"
     tRNA            1525..1599
                     /locus_tag="PK125_09245"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1557..1559,aa:Cys,seq:gca)
     gene            1766..2893
                     /locus_tag="PK125_09250"
     CDS             1766..2893
                     /locus_tag="PK125_09250"
                     /inference="COORDINATES: protein motif:HMM:NF024813.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HPB38327.1"
                     /translation="MGNTNVIFLEVIEWFDESGKEIVHRIPETGSGEIKWGAQLIVRE
                     SQAAVFFYNGKAYDALGSGRHTLTTGNIPILNKIMSVPWGMTSPLRAEVYFVNMKVFI
                     SLKWGTRDPVAFKDSELGLIRLRSFGVFNVQVIQPVLFVNTLVGTQGVYTTTEIESYL
                     NQVIVSRFNDYIGGNLDTVLNLPGRYDSIADGLTKTLQEDFSRFGLCLSELYINAVTP
                     PADVQKAIDDKSRMGLFEDMNRLLQMKSAMAMEKAAESQGGAGAGVGMGLGFMMPSMF
                     ADAMKASQMPVVTGPQVLCPDCKNPVGQDAKFCPSCGHQILVISQCPQCGKNLPPTAR
                     FCPKCGQETGKKPEAKKCPHCGTENLPSSVYCDQCGEKLER"
     gene            2893..4137
                     /locus_tag="PK125_09255"
     CDS             2893..4137
                     /locus_tag="PK125_09255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB38328.1"
                     /translation="MGSSWIVEHRCTQCGAPITLEEADRYFACPFCRVRICLTTDDHF
                     RYYLQPMDNPPEEIFYVPFWRVRGIWFICDDELEIKSSIVDATYCGFHGGPYPESLGL
                     RPQALKLRFAGPESGSIFPEPPQEFNSIFERVEGGLPHYHISNSPVRVFHKSFLGEKV
                     SIVYAPFFLKNRALHDGILGKPLKALRDGMSNVPPQGSIGRPLTVIPAMCPECGSDLE
                     GGRDSIVFFCRNCNKAWYLTKNNLERKGFLVLNGNQEATSYLPFWRIKAQVDGLKVQT
                     MADLARLANLPVVVREEWESTGLFFWVPGFKVHASLFLRLARLLTLHQPGDFVDHEEP
                     LDAPQPVSLPVDDASDSITVIIASLIAMKKRIWPSLESVTISQLHADLVYIPFQEQEG
                     DYFNPSLKISVPKNSLKFGQNL"
     gene            4240..5454
                     /locus_tag="PK125_09260"
     CDS             4240..5454
                     /locus_tag="PK125_09260"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HPB38329.1"
                     /translation="MTGRDYSGLRIDKTRIMPQQRRNRRVYFGVIFALVIVLLMVLGL
                     TGFFSPSIKVDTSTTSLVYPSQALTQLNASGYVVPQRKAAVAAKVTGRIIALYVEEGS
                     RVKKDQIIARLEADDVAAQRDQARANLEVSKQNLEQARAELADAKASFQREEALLAQD
                     FTTKAAFDAAEARCKKAIAAASAAEAMVRANNAALKAANVVLDYALIKAPFDAIVLTK
                     NADIGDIVTPIGAAANAKAAVVTIADLSSLQVEADVAESNLEAIKMGQPTEVQLDAIP
                     DTRFKGAIHMIVPTADRTKATVMVKVKFLNMDSRILPEMSAKVAFLSREASNDEQQPR
                     LAIPQKAIRVRNGNTMVFVVRGNRVDEMKVSAGHQIGDMIEINSGLTKGEKVVINPPA
                     KLRNGSRIKLIQ"
     gene            5482..6186
                     /locus_tag="PK125_09265"
     CDS             5482..6186
                     /locus_tag="PK125_09265"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPB38330.1"
                     /translation="MVRKNPIVQIKHVSKSYRRGSQIVPVLTDITVDINEGEFLALMG
                     PSGSGKSTLLNLIAGIDRVDSGSILVDGVEITGLVETELANWRANHVGFVFQFYNLIP
                     VLTAFENVELPLLLTRLTRKERKKHVEIALAVVNLSHRLAHYPAQLSGGEQQRVAIAR
                     AVVTDPTILVADEPTGDLDRKSAEEIMDLMDGLNRELGKTIIMVTHDPRAAQKAYVIR
                     HLDKGVLNDVHHQNSV"
     gene            6161..7318
                     /locus_tag="PK125_09270"
     CDS             6161..7318
                     /locus_tag="PK125_09270"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPB38331.1"
                     /translation="MYIIKILFRNALRHKLRTTLTVVGVIIAVLAFGLLRTVITTWYM
                     SVEASAANRLWTRNSVSLILPLPISYKDKIRQIEGVRSVSHITWFGGIYIDEKNFFAQ
                     FAVEPQTYMEAFPEYYIPPEQKAAMLRDRKGAVAGRKLAQRYGWKLGNVITIKGTIYP
                     GTWEFTLRGIYEGTRKDADETVFFFHWDYLNETLKKTAKGMEDQVGSFIVITKNLEDV
                     TDVVQRIDNSFKNSLAETLTETEKAFVLGFISMTDAIITAIEFVSFVVIAIILAVVAN
                     TMAMTARERIGEYAILKTLGFGWFKIAGLIFGESMVISMMGCALGIGLTFPIADVFGA
                     AMGQYFPAFNVTLETIYMDIATSIIVGLLAAIIPCWHAIKTPVAGGLRRIG"
     gene            7376..8485
                     /locus_tag="PK125_09275"
     CDS             7376..8485
                     /locus_tag="PK125_09275"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPB38332.1"
                     /translation="MITTSGMALVVFVFATILMLVQGLQQTLVQTGFEDNLIVIRRAS
                     GSEVQSGIDRYQASIIETDSAVASGSDGERFLAKEMVVLITLPKKGHAGSSNVTLRGV
                     TKKSLELRPQVKLVKGRMPNAGSTEIITGQSLVRRFDGVNIGESIRFGKRDWTVVGAF
                     DAGSTGFNSEIWGDSDQLMQAFRRPVYSSVLFRLRDHDDFDAVKVRLESDPRLTVDIK
                     REARYYEEQSEVMAKFLRILGISITVIFSLGAIIGAMITMYTSVANRIGEIGTLQALG
                     FQRTSILGAFLVEALLLGLAGGITGLVFASLMRFVKISTVNFQTFSELEFGFALSFEI
                     VCMVLAFALIMGFAGGVLPAIRASRMNIVDALRES"
     gene            8654..9430
                     /locus_tag="PK125_09280"
     CDS             8654..9430
                     /locus_tag="PK125_09280"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF013509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HPB38333.1"
                     /translation="MKLFSIVVLGLLVLASQVYGVDEALKVDKDKLSYTFGANFGKSL
                     KQQEIEINTDMFIKGIKDGLTGDKMLLTDQEMKDTMMAFQKEMAAKQAEKRKALAEKN
                     KKEGEAFLSANKANEGVKTLPSGLQYKVITEGTGKTPKATDSVVTNYRGTLIDGTEFD
                     SSYQRKNPATFRVSGVIKGWTEALQLMKEGSKWQLFVPPELAYGERGAGPKIGPNAVL
                     VFEIELVSVKEESASPPPKPGAKPGAAKPGIPAPKPAPTK"
     gene            complement(9535..10821)
                     /gene="larA"
                     /locus_tag="PK125_09285"
     CDS             complement(9535..10821)
                     /gene="larA"
                     /locus_tag="PK125_09285"
                     /inference="COORDINATES: protein motif:HMM:NF033504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050043 - lactate racemase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-dependent lactate racemase"
                     /protein_id="HPB38334.1"
                     /translation="MEEHYVLCEGEKLCFSLPPQWKVLTAEDKPFIAGVKDSLVEVNR
                     ALDNPIGSPKLEEIAKPGMEVALLFDDLQRPTPVNLVMPEMLNRLNRAGIPDNRVWAV
                     CAIGTHPVYSREELERKVGPEVSSRLKERLVSHDPYSPENVVIGRTHRGTLVEINPYV
                     AFADLVIAVGECMPHPCAGFGGGFKIVLPGVASYRSVAEHHFTWMRHRKSRVNILDGN
                     PWYEEIVDAGQIARLSFKLDCIINEKREIIRTFAGEPFAEHRAASEYASSLYYVPLPK
                     IADVTIIAAYPLEIGVQATKALTMAGFCTRAGGTIIWVAPQKESGPLMPLVRHMSSSE
                     TASDFHRRLIRGDIPEDLTSYGISFIMQIVFFKELAEKFNVIHVTEGLPSEVVRMMKF
                     THTTSLPEAIDIVARKEPQADVAIFPSGGNIIPGIV"
     gene            complement(10842..11840)
                     /gene="dctP"
                     /locus_tag="PK125_09290"
     CDS             complement(10842..11840)
                     /gene="dctP"
                     /locus_tag="PK125_09290"
                     /inference="COORDINATES: protein motif:HMM:NF015445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein DctP"
                     /protein_id="HPB38335.1"
                     /translation="MKKKLFLVFIAVCFALTVCMMCGQALAAEVVTLKAVTAWPKTSS
                     ENKAFFIFTELVEQMVAKKYPGELKINYIGGPEAVKNTDQAQAAQRGMVDMVFTTNAY
                     YLSVLPEVDAYKLSDFTPAEERTKGAYKYMNELHEKKGLYLLGRLGLSEKFYLYLKKP
                     IQSADLKGLNVRVSPMYLQIIKGLGGNPVVIPPTEVYVALERNVVDGFCWPAVGIRDW
                     GWHKQVKYIVEPGFYQVPNPLIMSLNTWKKLPKKLQDLLTEAASEAEKKAVAYFDQLA
                     KDERPILIKEGLQVINLPAAEQQKFLKVGSEEGWKDIIQKNPQTGPELKKLLTKGS"
     gene            complement(11916..>12106)
                     /locus_tag="PK125_09295"
     CDS             complement(11916..>12106)
                     /locus_tag="PK125_09295"
                     /inference="COORDINATES: protein motif:HMM:NF018513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HPB38336.1"
                     /translation="GLITPPFGVTLFVMKGVAPPDVTMADIWRAIIPYVIIDILCIFI
                     VLMMPSIATVVPNLMGMK"
BASE COUNT         3357 a         2715 c         2912 g         3122 t
ORIGIN      
        1 accctgcaga ttcaggtttc atccaaacag cgaaatattg acgagtcaat tgtcactcca
       61 cgccggttct ataggggcct ttattttggc ttggagtgca cagaacctat cgaaagaatt
      121 ccttcaggtc agcagcgagc tcttcaggcc tcacaacact cacgggttcg ctgagaagga
      181 cctgaaaaaa attcagatca ggtgcataat cccttgtgtt aagaaatgta cgagcggcaa
      241 ttcggaagtg acatgcaaat gactgctgac cttcaggacc aaaaaagagc gatgtattaa
      301 agctgtaaat gtcagtcgat atgtagtatt tgaagatttt aagaagaccg gccacgaggt
      361 cttccatatg tgtctcggca aactgactga catcaaagac ctcaggaaag acacacataa
      421 tctctccaag caggcccatg gagacaaagg aagaaagcca gtaagaggag ccaatctgac
      481 cgataaacct tttgtcctgc aaccgttcga cctctatgaa ttcccgccag tagttcgtgc
      541 cataggagtt gtaaaatgca tttgaagctt taagctcatc tgaaaattgg tttcctgggt
      601 ttgatgtggc aaaatactgt tgatgcggat ggacaagacc accaccagag ggaggcatat
      661 aattccatgt cataaggtga tagggttgtt cagggcttat agctttgatc cgttggagaa
      721 agtgaatccc taaccaaaaa gcgtcaaaca gcctttgttc atttaagtcc ttcagatgga
      781 ccacatgatg gtctgtcata ataagaacac cactgtgaac gtcatatgga aaaagattgg
      841 ggataagaca ggcctcgttg cgtatgagtc tcccctcaga cagtatgtcg tctttaaacc
      901 ttggggtaac tctgtctctg tgttcgagac aaaacgggca gaatcctttg atttcagggg
      961 tggcatatgc atcgaggctc agtttctggg gtttgattgc accaaagtgg gaaaaatgac
     1021 cagtcctgcc tgttaagggg tcaattcgca cttcaaaacg ttctgtcaca gggctaaagc
     1081 ctgttctggg atgaagaaaa gtgctttctg attggaagac ctggaaacgg atacgcgaag
     1141 acatcatgta atcctcctgc gtggtaattt catatagaaa cgaggacact ataccagatt
     1201 ttggccactt aattcattac gaatatcctc ggcttataac atgaagtggc gggcaaaaac
     1261 atatgggcca gattgccttg ggtagtcttg attagaaaga tttgacatgg ggtatccgtt
     1321 tatattataa gtaattacag ggcgggaata actcagcggt agagtgcaac cttgccaagg
     1381 ttgaagtcgc gggttcaaat cccgtttccc gctccacgat aaatcagctg attgcgttta
     1441 gctcaaagtt tttagtcaaa acgcgaaaat tgatttactg atttgctatc aactatgagc
     1501 tttcagctat aagctacact gcccggcggc atagccaagc ggtaaggcga cggtctgcaa
     1561 aaccgttatt ctccggttca aatccggatg ccgcctccaa cagacctgta caccataaag
     1621 aacaattcag gcaaaacaca acatcacttc agttgttaag tttccctaag gctatccgaa
     1681 aaatattgaa gattccccgt aggatgtgac acaagcatag aaataggata ggcaggcttg
     1741 caggcggggc aatatggagg caactatggg aaatactaac gttatttttc ttgaagtcat
     1801 tgaatggttc gatgagagcg ggaaggagat agtccataga ataccagaaa caggctcagg
     1861 ggagatcaag tggggtgcac aactcattgt gagagagagc caggcagcag tcttttttta
     1921 caacggaaag gcgtatgatg ccttgggatc tggtagacac acactcacga cgggtaacat
     1981 tccaattctc aacaaaatta tgagtgttcc ctgggggatg acaagcccac ttcgtgcaga
     2041 agtctacttt gtaaatatga aggtttttat cagtctcaag tggggtaccc gtgacccggt
     2101 ggctttcaaa gattctgagc ttggccttat ccgtctcaga tcattcggag ttttcaatgt
     2161 acaggtcatt caacctgtgc tctttgttaa cacccttgtc ggaacccagg gggtatatac
     2221 aacaactgaa atagaatcat acctgaacca ggttattgtc tctcgtttca atgattatat
     2281 cggggggaac cttgatacgg ttctaaattt gccaggaaga tatgactcaa tcgcagacgg
     2341 attgacgaag acgctccagg aagacttctc ccgattcggt ctctgcctgt ccgaacttta
     2401 tattaatgca gtaactcctc ctgcggatgt ccagaaggca atcgatgaca agagtcgcat
     2461 gggactcttt gaagacatga accgcctcct gcagatgaag agcgcaatgg ccatggaaaa
     2521 ggcagcagag tcccagggcg gggccggggc tggagtgggc atgggacttg gtttcatgat
     2581 gccttccatg tttgctgatg ctatgaaggc atcacagatg ccagtggtaa cgggcccaca
     2641 ggttttgtgc cctgactgca aaaaccctgt tggacaggac gcaaaatttt gtccctcctg
     2701 cgggcaccag atactggtaa tcagccagtg cccccagtgc gggaaaaatc ttccccccac
     2761 ggcacgtttt tgcccgaagt gtggtcagga aacggggaaa aagcccgagg cgaagaaatg
     2821 tcctcactgc ggaacagaaa acctcccatc ttcagtgtac tgcgaccaat gtggtgaaaa
     2881 gctcgaacga taatgggaag ctcatggatc gtcgaacatc gctgtaccca gtgcggcgcc
     2941 ccaataacac tagaagaggc agaccgctat tttgcctgcc ccttttgcag ggtaaggatc
     3001 tgcctcacca cagatgatca tttccgttac tacctccagc caatggacaa cccaccggaa
     3061 gagatattct atgtgccatt ctggagggta cgagggatat ggtttatctg tgatgatgag
     3121 ctggagataa agagttctat tgttgacgca acctattgcg gatttcatgg agggccatac
     3181 ccggagtccc ttggcttaag accacaggcc cttaagctca ggtttgccgg tcccgaatca
     3241 gggtcgatct ttcctgaacc gccccaggaa ttcaactcca tttttgaaag agttgagggt
     3301 gggttgcccc attaccacat cagtaattca ccggtgcgcg tatttcacaa gtcttttctc
     3361 ggagagaagg tgagcattgt ctatgcccct ttttttctga aaaacagggc tttgcatgat
     3421 ggtattctcg ggaagccact gaaggcactc agggatggga tgagcaatgt gcctccccag
     3481 ggaagcatcg ggcgcccgct gacagtaatc ccggcaatgt gcccggaatg tgggtcggac
     3541 cttgagggag gaagggatag catcgtcttt ttctgcagga actgcaacaa ggcatggtac
     3601 cttacgaaaa ataacctgga acggaaaggc tttctagtgc tcaatggaaa ccaggaggca
     3661 acgtcttacc tccctttctg gagaatcaag gcacaggttg atggtcttaa ggttcagacc
     3721 atggctgacc ttgcaaggct ggcaaacctg cctgttgttg ttcgagagga atgggaatca
     3781 accggcctct ttttctgggt tcccgggttc aaggtccacg catccctttt tctcaggctt
     3841 gcacgccttt tgaccctcca ccaaccagga gactttgttg accatgaaga gcctcttgat
     3901 gctcctcaac cggtttccct gcctgtggat gatgcaagtg atagcatcac ggtgattata
     3961 gcaagcctta tcgccatgaa gaaacgtatc tggccgtccc ttgagtccgt aacaatatca
     4021 caactacatg ctgaccttgt ctacattcct ttccaggaac aagaaggtga ttacttcaat
     4081 ccgtcattaa aaatcagcgt gccaaaaaat tctttaaaat tcggtcagaa tctgtagtaa
     4141 ggcaaaagac atataagctc tcaccagcct taattctatt gacttatggt agactgatat
     4201 ttaacattat ggaaaaaatt cacaaaggaa tacaactcat tgacaggacg agattattct
     4261 ggtctcagaa tcgataaaac aagaatcatg ccccaacaga gaaggaatag acgagtttat
     4321 tttggagtaa tttttgcgct ggtgattgta ctcctcatgg tactgggcct gaccggtttt
     4381 ttctcaccat ctatcaaggt agacacgagc acaacctcac ttgtctatcc ctcgcaagcg
     4441 cttacccagc ttaacgcaag cggttatgtt gtcccgcaac gaaaagccgc tgttgctgcc
     4501 aaagtgactg gaagaatcat agctctttat gtagaggagg gaagtcgcgt caaaaaggac
     4561 caaatcattg ctcgcctgga agcagatgat gttgctgctc aaagggacca agcccgagca
     4621 aacctagagg tgtcgaagca gaatcttgag caggcaaggg ctgaactggc tgatgcaaaa
     4681 gcttcttttc aaagagagga agctctcctt gctcaggatt tcacgacgaa agcggcattc
     4741 gatgcagcag aggcgcgctg taaaaaggcg attgctgctg cttccgctgc agaagcgatg
     4801 gtcagagcga acaatgctgc tctaaaggca gccaatgttg tattggacta tgcgctcatc
     4861 aaggcacctt ttgatgccat agtgcttaca aaaaatgcag atatcggtga cattgtgaca
     4921 cccatcggtg cagcggcgaa tgcaaaggct gcagtggtaa ctattgctga cttgagttcc
     4981 cttcaggtag aggcagatgt tgctgaatcg aatctcgagg cgattaagat gggacaacca
     5041 actgaggtcc agcttgatgc aataccagac acccgtttca agggcgcgat tcatatgatc
     5101 gtgcccacag cggacaggac aaaagcaacg gtaatggtga aggtgaaatt cctcaacatg
     5161 gattcaagga ttcttccgga gatgagcgcg aaagtagcct ttctttctcg agaagccagt
     5221 aacgatgaac agcaacccag acttgctatt cctcagaaag caataagagt aagaaatgga
     5281 aacacgatgg tttttgtcgt gagaggaaac agggttgatg aaatgaaggt ttcagctggg
     5341 catcagattg gggatatgat agaaatcaat agcggtctta cgaagggaga aaaggtggtc
     5401 attaacccgc ctgccaaact cagaaatgga tcaagaatta agctcataca gtaataatat
     5461 ctgcgcattt cgagtcgaaa tatggtgcga aaaaacccaa ttgtccagat taagcacgta
     5521 agtaaatcat atcgtcgtgg cagccagatt gtgcccgtgc tcaccgacat cacagttgac
     5581 attaacgagg gagagttcct tgccctcatg ggaccatcag gctcaggcaa gtccacgctt
     5641 cttaatctta tagcaggaat cgacagggtt gacagcggaa gcattcttgt cgatggtgtt
     5701 gagatcacag gcctggttga aacagagctt gcaaactggc gggccaatca cgtgggtttt
     5761 gttttccagt tctataacct gatcccagtc ctgaccgcat tcgaaaacgt agaacttccc
     5821 cttctcttaa caaggcttac aagaaaagaa aggaagaagc atgttgaaat tgcccttgcg
     5881 gtagtaaatc tttcccacag gcttgcccac taccccgcac agctttctgg aggcgagcaa
     5941 caacgggttg ccatagcgcg agctgttgtt acggacccaa caatactggt ggcagatgaa
     6001 cctacaggcg atcttgatag aaaatcagct gaagagatta tggatcttat ggatggtctg
     6061 aaccgggaac ttggtaagac aattatcatg gtaactcatg acccaagggc cgctcaaaag
     6121 gcctatgtta ttcgtcatct cgacaaaggt gttctcaacg atgtacatca tcaaaattct
     6181 gtttagaaac gctctacgtc acaaacttcg caccactctg acggttgttg gagttatcat
     6241 tgcagtcctg gcgttcggcc tccttcgtac agttattacc acatggtaca tgagcgttga
     6301 agcctcggca gcaaacaggc tttggacacg aaactctgtg tctctcatcc ttccattacc
     6361 catatcatat aaagataaga tcagacagat tgaaggtgta cgatccgttt ctcatatcac
     6421 atggttcggt ggaatctata ttgatgaaaa gaattttttt gcccagtttg ctgtagaacc
     6481 tcaaacatat atggaggcat ttcctgagta ctacatccct cctgaacaaa aagctgccat
     6541 gctaagagat cgaaaaggcg ctgttgcagg gaggaagctt gcccaaagat atggatggaa
     6601 gttagggaac gtaattacca tcaaaggaac gatctaccca gggacttggg agtttacact
     6661 gcgcggtatt tatgagggaa caagaaagga tgcagatgag acagtgtttt tctttcattg
     6721 ggattatctg aacgaaactc tgaagaaaac ggctaagggc atggaagatc aagtaggctc
     6781 tttcatcgta attacaaaaa acctcgagga tgttacagat gtagtccaac gcatagataa
     6841 ttcgttcaaa aactctcttg cagaaactct cactgaaacg gagaaggcgt ttgttctcgg
     6901 cttcatctcc atgactgatg ctatcataac cgctattgaa tttgtttcct ttgtcgttat
     6961 tgccattatc cttgcagtgg tagcaaacac aatggctatg acagcgcggg aacgcatagg
     7021 cgagtatgca attctaaaaa cattaggttt cggctggttt aagatagcag gtctcatttt
     7081 cggagaatct atggttatct caatgatggg ctgtgctctt ggaattggac tcacctttcc
     7141 cattgcagat gtctttgggg cggcaatggg gcagtatttt ccagccttta atgttaccct
     7201 tgaaactatc tatatggata ttgctacatc aataattgtt ggtcttcttg cagccatcat
     7261 cccgtgttgg catgctataa agactcctgt tgcaggtggt ttaaggagaa taggttaagt
     7321 gaaaatcccc cttttgtata gcattagaaa cctgtggaca aggaggctca ccacggtgat
     7381 aactacatca ggaatggcac tggtagtctt tgtttttgcg accattctga tgttggtgca
     7441 agggctccag caaacccttg tgcaaacagg gtttgaagac aacctgattg tcattcgcag
     7501 ggcttccggc tcagaggttc aaagtgggat agatcgttac caagcatcca ttattgaaac
     7561 agactctgct gtagccagtg ggtctgacgg cgagaggttt ttagcaaagg agatggtggt
     7621 tcttataacg cttcctaaaa aaggacacgc tggatcttca aacgttactc ttcgcggagt
     7681 tacaaaaaaa tccttggagc tgaggccaca ggtaaagctt gtaaaaggcc gcatgcccaa
     7741 tgcagggtct accgaaataa taacaggcca gagtttggta aggcgttttg atggggtcaa
     7801 tataggtgaa tcaataaggt ttggtaagcg agattggact gtggtaggtg cttttgatgc
     7861 agggagtacc ggctttaact cggaaatctg gggcgattca gaccagctta tgcaggcctt
     7921 caggagaccg gtatattcat cagttctttt cagactgaga gatcacgatg attttgatgc
     7981 agttaaagtt aggctggaat cagaccccag gctcacggtg gacataaaaa gggaggcccg
     8041 ctattatgag gaacaatctg aggtgatggc caaatttctt cgtattcttg gtatctctat
     8101 caccgttatt ttttcccttg gagctatcat tggagccatg attactatgt atacctccgt
     8161 agcaaacagg attggcgaga taggaacctt acaagcatta ggttttcaga ggaccagcat
     8221 ccttggcgca tttcttgtag aggcactgct tctaggtctt gccggaggca ttacaggcct
     8281 tgtttttgca tccctcatga ggtttgttaa gatctcaacg gttaattttc aaaccttttc
     8341 agaacttgag ttcggctttg cacttagttt tgagattgtt tgtatggtgt tggcttttgc
     8401 tttgattatg ggttttgcgg ggggtgttct gccggcaatc agagcatcgc gcatgaatat
     8461 agtggatgcc ttgagagaat cctgataaga caactcaatg ctctccggtc atagctgaca
     8521 gtgacggcat agagaatgat aagtcttgtc agcagttttt ctgagcgtac agcttcctgc
     8581 aatgagctac aaagattgac atgttcccga aaaacatgat aaatgacaaa cattatcata
     8641 acaaggaggg tgtatgaaat tattttctat tgtagtgcta ggtttgttgg ttcttgcgag
     8701 ccaggtttat ggagttgatg aggccctcaa ggtagataaa gacaagctca gctatacatt
     8761 cggggctaac tttggcaaaa gcctgaaaca acaagaaatt gaaataaaca cagatatgtt
     8821 tataaaagga ataaaagacg gcttaacagg cgacaaaatg ctccttactg accaggaaat
     8881 gaaagacaca atgatggctt ttcagaagga aatggctgca aagcaggcag agaaacgaaa
     8941 agcacttgcc gagaaaaaca agaaagaagg ggaagccttc ctttcagcga ataaggccaa
     9001 cgaaggcgta aaaaccctcc ccagcggtct gcaatataaa gtgattaccg agggtacagg
     9061 aaaaaccccc aaagctactg actctgttgt aacaaactat agaggaacac tgatcgatgg
     9121 aacagagttt gatagctctt atcagcgcaa aaaccccgcc acgttcagag tgagtggagt
     9181 tataaaggga tggacagaag cgctgcaact gatgaaagaa ggaagcaagt ggcagctttt
     9241 tgtcccgcca gaactggcct atggtgagcg cggagcagga ccaaagattg ggccgaatgc
     9301 ggttcttgtc tttgagatcg agttagtttc agttaaagaa gagagtgctt cgccgccacc
     9361 taaaccaggg gctaaaccgg gtgctgccaa gccaggcatt cccgcaccaa aaccagcacc
     9421 aacgaaataa gaaaaatggc tataggcaag gggtaatggg caatgggaaa aaacaagaga
     9481 ccatactcgc tctttcaacc cattgcctag agcgcctctc gcctatggcc tgatttatac
     9541 aatccccggt ataatatttc ctcctgaagg aaaaattgcc acatctgcct gtggttcctt
     9601 cctggctact atatctatgg cctccggtag gcttgttgtg tgagtgaatt tcatcatacg
     9661 caccacttcg cttggcaggc cttctgttac gtgaatgacg ttaaacttct cagcaagttc
     9721 cttaaagaag acaatctgca tgataaatga gatgccataa gacgttagat cttcaggaat
     9781 atcacctctg atgagtcgcc gatgaaaatc acttgctgtt tccgaacttg acatgtgcct
     9841 taccaatggc atgagaggtc cagactcctt ttgcggtgca acccagatga ttgttcctcc
     9901 tgcccgggta cagaagccag ccatggtgag cgcttttgtc gcctgaacgc caatctccag
     9961 gggataggct gctataatgg tcacatcagc gatcttggga aggggcacat agtaaagaga
    10021 tgaggcgtat tcagatgctg ccctatgctc ggcaaagggc tcaccagcaa aggtcctgat
    10081 gatctccctt ttttcattga tgatgcagtc gagtttgaag gagaggcgag ctatctgtcc
    10141 tgcgtctaca atctcttcat accaggggtt tccatcgaga atgttcaccc tgcttttcct
    10201 gtggcgcatc caggtgaagt ggtgttcggc aacagaacgg taggaagcaa ctcccggtaa
    10261 tacaatcttg aatccacctc cgaaacctgc gcatggatgg ggcatacatt cccccacagc
    10321 aataacgaga tcggcgaaag ctacatatgg attaatctcc acgagagttc ctctgtgagt
    10381 cctcccgatt accacgttct caggagagta agggtcgtgg gaaacaagcc gttctttcaa
    10441 gcgtgatgac acttccggtc ccacctttct ctcaagctct tcccgagagt atacggggtg
    10501 agtaccgatt gcacagacag cccatacccg gttatctggg attcctgccc tgttcagcct
    10561 gttgagcatc tcaggcatga caaggttgac aggagtcggt ctctggagat catcaaaaag
    10621 gagagccact tccatacctg gttttgctat ttcttcaagt ttcggagatc caatggggtt
    10681 gtcgagggcc cgattgactt ctaccagaga atctttaacc ccggcaatga atggtttatc
    10741 ctccgcagtg aggactttcc attgtggagg aagggagaag caaagctttt ccccctcaca
    10801 caaaacataa tgttcttcca taactgcctt cccgggagac actaactccc ttttgttaga
    10861 agttttttca gctcagggcc ggtctgtgga ttcttctgaa taatgtcctt ccatccttct
    10921 tcagagccaa ccttgaggaa tttctgctgt tctgcggcag gaaggttaat tacctggagc
    10981 ccttccttga taaggatagg cctctcatcc tttgccagtt gatcaaagta ggcaactgcc
    11041 ttcttttcag cctctgatgc tgcttcggtg agaagatcct ggagtttctt tggtaacttt
    11101 ttccatgtat ttaaactcat gatcagggga tttggaacct gataaaagcc aggttcgaca
    11161 atgtatttaa cctgtttgtg ccacccccag tcccgaatgc cgactgcggg ccagcagaat
    11221 ccatcaacaa catttctttc gagggcaaca tacacctcgg ttggtggaat gacgacaggg
    11281 tttcccccaa gcccttttat gatctgtaaa tacattgggg agaccctaac attgaggccc
    11341 ttgaggtctg cactttggat cggtttcttg agatagagat aaaacttctc gctgagcccc
    11401 aacctgccta gaagatagag ccctttcttc tcgtgaagtt cattcatgta cttgtaagcg
    11461 ccctttgtcc tttcctcagc aggggtaaag tcagaaagtt tgtatgcatc gacctcaggg
    11521 agaacagata aataataagc gtttgttgtg aagaccatgt ctaccatgcc acgctgcgca
    11581 gcctgtgcct gatcggtatt ttttactgcc tcaggaccac caatatagtt gatctttaac
    11641 tcgccgggat atttcttagc taccatctgt tcaaccagtt ccgtaaaaat gaagaatgcc
    11701 ttgttctcac tcgatgtttt tggccaggca gtcacggctt tcagggtaac tacctcggca
    11761 gccaatgcct gcccacacat catgcaaacc gtcaaagcaa aacaaaccgc aataaacact
    11821 aaaaacaatt tctttttcat tttttgcctc ctcattattg atttgttaag cttttgttcc
    11881 catcttctta acagcttaat ttcacaattt ctgccctact tcatccccat caaattcgga
    11941 acaactgttg cgatggaagg catcatcagc acaataaaaa tacagagaat atcaatgatc
    12001 acataaggaa taattgctct ccagatatcc gccattgtca catcaggagg ggcaacccct
    12061 ttcataacaa aaagggtaac accaaaaggt ggtgttatca agcctg
//
LOCUS       DAOOMC010000084        12105 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdus sp. isolate AMR_MDS_2685
            NODE_5840_length_12105_cov_6.396100, whole genome shotgun sequence.
ACCESSION   DAOOMC010000084 DAOOMC010000000
VERSION     DAOOMC010000084.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864760
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdus sp. (wastewater metagenome)
  ORGANISM  Syntrophorhabdus sp.
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae; Syntrophorhabdus.
REFERENCE   1  (bases 1 to 12105)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12105)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,729
            Genes (coding)                    :: 2,708
            CDSs (with protein)               :: 2,708
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 7 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12105
                     /organism="Syntrophorhabdus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5840_length_12105_cov_6.396100"
                     /isolate="AMR_MDS_2685"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2699756"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..232
                     /locus_tag="PK125_09300"
     CDS             <1..232
                     /locus_tag="PK125_09300"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HPB38337.1"
                     /translation="WATTTSPVKFHEATRHSYATRMAKILPVQMVQILLGHSDIRSSM
                     IYYHGDIETVKNAIPASYYSHTKIEGGRSEKP"
     gene            complement(250..339)
                     /locus_tag="PK125_09305"
     tRNA            complement(250..339)
                     /locus_tag="PK125_09305"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(303..305),aa:Ser,seq:tga)
     gene            complement(452..1438)
                     /locus_tag="PK125_09310"
     CDS             complement(452..1438)
                     /locus_tag="PK125_09310"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: protein motif:HMM:NF006829.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HPB38338.1"
                     /translation="MKRVAIVGTGSYLPETIMTNFDIEKFLDTSDEWIYTRTGIKTRR
                     IADKDWAVSDLSKIACERAMDMAGLKADDIDLIILATITPDTHCPAGANWLEAKLGCT
                     KAVSFDITAACSGFIFALHVADKMIKSGANNTVLVIGAEIMTRVVNWKERESCVLWGD
                     GAGAAVVTASDSGAQVLSTHVHTDGKNGDTLLMPGGGSKTTPISYESVDRGLHFLKMI
                     EANRSFKVAVNRFAEAVEEAVEINGKKVSDVDVIIPHQANLRIIQGMAKRLKVDIGKV
                     YMTIEKYGNISSATVPIALDEAVRDGTIKKDSLVCLTAFGGGLTWGSSLIKW"
     gene            1540..1692
                     /locus_tag="PK125_09315"
     CDS             1540..1692
                     /locus_tag="PK125_09315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB38339.1"
                     /translation="MGRRGGGRWEVKGRRWEVDSGGKEDTTCMPKPGTGRQKPEKIVD
                     IRMIYF"
     gene            1713..2612
                     /locus_tag="PK125_09320"
     CDS             1713..2612
                     /locus_tag="PK125_09320"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HPB38340.1"
                     /translation="MTKDYVDIQGFFIILLITMLWGLNYPAVKISNTGLSPVFTTFLR
                     SVIATCCGVIYCIVVRQPLFQRGILLFHGAMTGILFGIEFIFLYYGLLYTNASRAVIL
                     LYLSPFVVTIGAHFFLKERLTILKGVGLILAFLGIFLVFKGKPSEYNKLMLIGDLCEI
                     MAAVFWGATTIYIKKYLAQKVHPINTFLYQLAFSIPIMLVGALLLEHTWIREITTPVA
                     LSLFYQSVIVAFASYLAWFKLIHIYPVAQLSVFTFLTPIFGVFASAIIMKEQLTIGLL
                     AGLTFVCIGIYCTNYQKRQRAQS"
     gene            2599..3576
                     /locus_tag="PK125_09325"
     CDS             2599..3576
                     /locus_tag="PK125_09325"
                     /inference="COORDINATES: protein motif:HMM:TIGR00374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HPB38341.1"
                     /translation="MRKAKVITIAGFIVSIVLLYFSLKDIRFHDITTTLQKADPWLIP
                     LPIIFILCAVLLCAFRWSRLIGNSVHFLDAFAALMIGLFVNNVLPARIGEVARAYVIS
                     KKRKLSFTFSFSTVLLDRFFDLTGLLLLAFVFFPEKSLPPAVSRSIYILIALLIVCVG
                     CIILLSRERFANKLAGMSQRIRRPLFTKLTERVFKIQENLKRINSPFNLLLTVTISFC
                     TWFSMTIALYLVILMLGIQVDFCHVPFVCALLNLGLTIPSSPGYVGVYQFLLVYLLTI
                     FGVPKHEGFTISLIYHAMWFVPYNIIGFIYLLKEHLKIRELRKFEGSKD"
     gene            complement(3585..4670)
                     /locus_tag="PK125_09330"
     CDS             complement(3585..4670)
                     /locus_tag="PK125_09330"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPB38342.1"
                     /translation="MSLLVDLLSKTKTKDLRKEIPPDLRKTVINGANKRKTRRKVIFL
                     STLVLVVFLAGFGTIYVMDIFKSSLSVSIAAKTDARLPPQSPVASDNKPRNPFDEVKE
                     PVQHVTAVPKILLPHTGSQEKLTPAMDSFPKVAEKHTREGQPVIQAMAKTQAPVGLKL
                     SSVETQSAEKGEGKTNKDVFLLAARTHEGKGELRQAIDCYMKALELDPANYVAMNNIA
                     GIYIRLKLFDESLSYANKALGIKMNYIPSLINAGIAHISLGNLLDGESLLVRATALDP
                     LNKVVLFNLAVLYEKQGNYGKAFENYYKLSQMRDADGCLGAARILERQGHYSETARFY
                     RDILSIETASPSARELANQRLTQLSKQ"
     gene            complement(4675..5169)
                     /locus_tag="PK125_09335"
     CDS             complement(4675..5169)
                     /locus_tag="PK125_09335"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HPB38343.1"
                     /translation="MGKRALAILFIFHPLFVFAFCFDEAAREYGLNASLLRSIATVES
                     NCNPSALSYNRNGSTDLGIMQINSSWIRPMGLNESELLKNPCYNVKTAAKILKKCVEK
                     HGYDWEAIGCYNALGIKARVKYSWRVFTELSRPENHMSGTSSSVSGPSSLHFAVREIS
                     ETDH"
     gene            5343..5951
                     /locus_tag="PK125_09340"
     CDS             5343..5951
                     /locus_tag="PK125_09340"
                     /inference="COORDINATES: protein motif:HMM:NF019575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HPB38344.1"
                     /translation="MIITKMTRNQKGFTLVELAIVLVIIGIILGAVLKGQALINNAKI
                     KRVYNQQREVLAAVYSYFDRYSFFPGDDPNSATRWAGVTAGNGNGLIAAGAGSTAPNY
                     ACTATGTEQCDLWNELRLSEFISGSGFLNPTNAYGGRIALTYYSVQGLLAHWIAFQNI
                     PYDACQSIDLQFDDGVYNTGGIRGTGNYGTATSGVFDLYFKF"
     gene            complement(6028..7254)
                     /locus_tag="PK125_09345"
     CDS             complement(6028..7254)
                     /locus_tag="PK125_09345"
                     /inference="COORDINATES: protein motif:HMM:NF017437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HPB38345.1"
                     /translation="MDYLEFFGLKEDPFTITPDLTYFYPSKEHNEILTALNYAVTQKE
                     GFFLATGEPGTGKTTILKVFIDEWKHKAEIALVLTPRLSPEEFLHVVLEDLKVSIKDK
                     NKNEMLKAFRDFLLSSISRGKAVIIVVDEAQNLSDETLEELRLLSNLETEREKLLQIV
                     LIGQTELRERLASDNMKQLSQRVMVKASLRPLTREETSDYINYRSMKAGKGLPVFEED
                     AKNLIFKYSKGVPRLINLVSSRALMAAYVDGSTDIKKHHVRYAIDHLTEREKRSYGWM
                     SYSKYAAIVVLTILIGVAGGLGFRRIAVEYKDQKIEKPVKLSKATEETKPVPEIQPEA
                     AKPKVLLAVVAVHSAILRQEPSIDSAPVTYVAKGTILKVVEEKAGENEIVWHKVKISD
                     GRECWISGKVVQLKEQ"
     gene            complement(7255..8472)
                     /locus_tag="PK125_09350"
     CDS             complement(7255..8472)
                     /locus_tag="PK125_09350"
                     /inference="COORDINATES: protein motif:HMM:NF012694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HPB38346.1"
                     /translation="MNYYFYKAVNIDGSLIDGIVEAEDQAAAYEDLTARNLRVLVVKK
                     ASKIAARLRQHFISRRVKRGDMVECIRNLSVMLTAGIPLLNALEDSIEATENRSLKSA
                     LTDAKKEIELGLNFSDALSRQRGIFPDILIRLVKVGESTGRLDKSLTDVADHLQRIED
                     LSQAIKRSLFYPVFAIVTTTGAMIFWFVYVLPKIMNVIQEMGVELPLITRALMVVSNA
                     ISTYWYVIIGIVILCVIAIQIMRTREKTRYYYDYMLLKLPIVKLIVYNKLLALFAEQM
                     RILIVAGLTIDRTLDIVAGVIDNLVFRRALVAVRENVLLGSRISDTLKDHNIFPPMVT
                     RMVSAGEKSGNLDTQFSFLSGYYIKRLDDISAKLGKMIEPILLSIVGLVFILILMAVL
                     LPVYDLVSKVGKM"
     gene            complement(8469..10115)
                     /locus_tag="PK125_09355"
     CDS             complement(8469..10115)
                     /locus_tag="PK125_09355"
                     /inference="COORDINATES: protein motif:HMM:NF012652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HPB38347.1"
                     /translation="MATFRLGDILKTKGLINDKQINISILHQKVTGDLLGDIFVRLGF
                     ITSKELGQTLAEQFGMEFIDLSEYPVSDDALRLIPKEIAEKEEFIPIELDEGLLSIGV
                     TNPSNITAVDRVVNITKNYPRVFLVDPDSFRDSLETSYFFLENPIQERMEEIVKEVVR
                     TGMATGQQIASMTELIIMEGIREKATDIHINPFLDVSTVFYRIDGVLQHRFSLPKVAH
                     GGIVSRTKIMSQIDIAETRLPQDGSFSFTFLDKSYDIRASTVPTIYGENVVMRILAGT
                     GYLLSIDKLGFEAKGTEKIRLLFQKPYGIVLIAGPTGSGKTTTLYSALREVNLLERNV
                     ITVEDPVEYKLSFVRQTQVNEKVGYDFALAGRNFMRQDPDVMLLGEVRDEETARIAIR
                     ASITGHLVLSTLHTNDAVTSIPRLLDLKVDRFLLSTSLLAIIAQRLLRKICRFCKVEY
                     SLTEEERAQFQEYGVSAEKGFRGQGCAKCGNTGYSGRTAIGEILIVDDEMRELIFSEA
                     PITAMRAAALNKGMILLREDGFRKASEGVTTLEEVIRVTG"
     gene            complement(10145..11785)
                     /locus_tag="PK125_09360"
     CDS             complement(10145..11785)
                     /locus_tag="PK125_09360"
                     /inference="COORDINATES: protein motif:HMM:NF012485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB38348.1"
                     /translation="MVKDTVRLKRFLCHSVVIFFFLLMTACSSYTVKKAIEIPSETKQ
                     VKAEPSRTVEMRAPEFIPVTEEISPLKTRIVDVVARNTPLQDVLHVIAEATNLNVVME
                     KGVVPETPVTISLRNVSAEDALHTLLSSAGYFYMIKDTMLFVKAYDTKVFEIGIPPVV
                     QSYNVEVGGNIFGGVTGNIAPVSTGGGASAATTGTASTEIKGTISQTLKSDEATFKFW
                     DSVEQSIANLLGQGTAGSSGPPGKGPRTADEHFTINRLTGTIIVTATKNNLEKVAQFL
                     NRIRKVMTRQVLIEAKVIEVTLSQGYQFGIDWNLTFSLGGKWSGSVGLGSTGFSSVVS
                     STSPVFSIGTGDDGITRTLSHGTIDSINIKGLVNALEQQGDVRLLSNPRINMMNGQTA
                     MLTVGRNTAYISQIESTTLQGTNPVVTFTVGTSSILSGITIGIVPFVDDVGEISLTIT
                     PIVSELITLDTKNIGSMANQLQISLPTVDLRELSTTAKVRDGQVVVIGGLTRRSERVK
                     ENQTPFLGKLPLLGGLFRSSDRGEVNTELVIILQPVLL"
     gene            complement(11767..>12105)
                     /locus_tag="PK125_09365"
     CDS             complement(11767..>12105)
                     /locus_tag="PK125_09365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB38349.1"
                     /translation="HLTSVERGILLFRQEKIMMKERHFTAVPALKNPMDQHLSEKKSY
                     PSVKLSDIAPREQQHVSLVLIRGEKRIAIIDNLVVREGDGISQGRVARIERNGVLIKN
                     REGEQWLKIR"
BASE COUNT         3188 a         2892 c         2476 g         3549 t
ORIGIN      
        1 atgggccacg acaacgagtc cggttaaatt tcacgaagca acacgacata gttatgcaac
       61 caggatggca aagatattgc ctgttcagat ggtgcagatc ctcttaggac attcggatat
      121 cagaagcagc atgatttatt accatggaga tattgaaacg gttaagaatg ctattccagc
      181 ctcatactat tctcatacta aaattgaggg gggtagaagt gaaaaaccct aataattcca
      241 agagaaatat ggcggaggga gtaggattcg aacccacggg gcgcgtaaac gcccaacggt
      301 tttcaagacc gccgctttcg gccactcagc catccctccg cttttttagc tctcagcgat
      361 caactttcgg ccgcacgtaa aaaacaatgt taataattat atactatttt cctcatagct
      421 caaagctata agcgaagaac tacaaactgc ttcaccattt aatgaggctg ctaccccatg
      481 tcaaaccacc accaaatgct gtcaggcaca caaggctgtc cttctttata gttccgtcgc
      541 gaacagcttc gtcaagggca atggggactg ttgcagatga tatgttgcca tatttttcaa
      601 tagtcatgta aactttgcca atatctactt taagcctttt tgccatgccc tgaataattc
      661 tcaggtttgc ctgatgaggt atgatcacat caacatcact caccttcttg ccgtttatct
      721 caactgcttc ttcaactgcc tcagcaaacc tgttgaccgc tactttgaaa gacctgtttg
      781 cctcgatcat cttcagaaaa tggagtcccc tgtcaacgct ctcataggaa atgggggttg
      841 ttttcgatcc tcctccaggc ataaggaggg tgtcgccatt ctttccatca gtatgaacat
      901 gggttgacaa tacctgtgcc cctgagtcac tcgctgtgac tacagctgca cctgctccat
      961 cgccccaaag tacacagctc tccctctcct tccagttcac tacccttgtc ataatctctg
     1021 ctccaataac aaggaccgta ttattcgcac ctgatttaat cattttatct gcaacatgga
     1081 gggcgaagat aaaaccagag catgcagcgg ttatatcgaa agaaacagct ttcgtacaac
     1141 caagcttcgc ctccagccag ttggctcctg ctggacaatg tgtgtcaggc gtaatggtgg
     1201 ctaaaataat tagatcaatg tcatcggcct tgaggccagc catatccatc gctctttcac
     1261 aagcaatctt tgataaatcg gaaactgccc aatctttgtc agcaattctc ctggtcttaa
     1321 tccctgttct tgtgtatatc cattcatctg atgtatcaag gaatttctca atatcaaagt
     1381 ttgtcatgat cgtctcgggg agatacgaac cagttccaac aattgcaacc cttttcatta
     1441 tgtgcccctt ttcaacaaaa cgtatttcag atgcctttta atagaacaga aaaaagcaga
     1501 gtatgtcaag ggaaacatgg atagaacagg gcgagtgaga tgggaaggag aggaggtggg
     1561 aggtgggagg tgaaaggtag gaggtgggag gtggatagcg gcggaaagga ggatacgacc
     1621 tgcatgccaa agcccggtac aggtagacaa aaacccgaga aaattgttga catcaggatg
     1681 atatatttct aaaacttgaa gagagatata tcatgaccaa agattatgtt gatatacaag
     1741 gctttttcat tattctcctc attacgatgt tgtggggttt aaactatcct gcagtaaaga
     1801 tttcaaatac gggcctatcg ccagttttca ccacattcct tcgctctgtg attgccacgt
     1861 gttgcggggt catttactgc attgtggtca ggcaaccatt attccagagg gggatccttc
     1921 tcttccatgg tgctatgaca gggattctat ttggcataga attcattttc ctgtactatg
     1981 gcctcctata cacaaacgca tctcgagcag ttatcctttt gtacctttca ccatttgtgg
     2041 tgacaatcgg cgcccatttt ttcttaaaag aacgtttaac tatcctgaaa ggagtggggc
     2101 tcatccttgc atttctcggc atcttccttg ttttcaaggg caagccaagc gaatacaaca
     2161 aacttatgct catcggcgat ttgtgcgaaa taatggctgc cgttttctgg ggtgccacaa
     2221 ccatctatat taaaaagtac cttgctcaaa aagttcaccc catcaatacc ttcctgtacc
     2281 aacttgcttt ttccattccc atcatgcttg ttggcgccct tcttcttgag catacatgga
     2341 taagggaaat cacaacgcct gttgccttat ccctcttcta ccagtctgtc attgtggcct
     2401 ttgcttcata tctcgcatgg ttcaagctca tccacattta ccctgtggca caactctctg
     2461 ttttcacctt tctaacccct attttcgggg tattcgcaag tgcaattatc atgaaagagc
     2521 aactgaccat aggtctcctc gcaggcctta cttttgtatg catcggcatc tactgcacaa
     2581 attatcagaa gaggcaacgt gcgcaaagct aaagtcataa ccattgcagg ctttattgtc
     2641 agcattgttc ttctctattt ttcactgaaa gacatcaggt ttcatgatat cacgacgact
     2701 cttcagaaag cagatccatg gctcatccca ctccctatca tcttcatatt atgcgccgtg
     2761 cttctttgcg ctttcagatg gtcacgactg ataggaaata gcgttcattt tcttgacgct
     2821 tttgcagccc tcatgattgg cctcttcgtg aataatgtac tgccggcaag gattggagaa
     2881 gttgcaaggg catacgtgat ttcgaagaag cgtaaacttt cattcacttt ctccttctcc
     2941 actgttcttc tggacagatt ttttgatctc accgggctct tgcttcttgc gtttgtcttt
     3001 tttcccgaaa aaagccttcc acctgccgta tcacgatcaa tctacattct aatcgcctta
     3061 ctcattgtct gcgtgggctg cataattctg ttgagcaggg agaggtttgc aaataagttg
     3121 gccggcatgt ctcaacgtat cagacggcca ctgttcacta agctgaccga gcgcgttttc
     3181 aagattcagg aaaacttgaa gaggattaat tcccctttta atcttctctt aacagtaacc
     3241 atatcttttt gtacctggtt ctccatgacc attgcccttt atctcgtcat cctcatgctg
     3301 gggatacagg ttgatttttg ccatgttcct tttgtctgcg ccctgttgaa cctcggtctt
     3361 acaatcccat cttcgccagg ctatgtgggg gtttaccagt ttctcctggt ctatctgctt
     3421 accatcttcg gtgttccaaa acacgagggt tttacgatat cacttatcta ccacgctatg
     3481 tggtttgtgc catacaacat aatcggcttc atctatcttc taaaagaaca tctcaaaatt
     3541 cgtgaattaa ggaaatttga agggagtaaa gactaaacga ttctttattg ttttgatagt
     3601 tgtgttaacc tttgatttgc caactctctt gctgaagggc tggctgtctc aatagatagt
     3661 atgtcccgat aaaatctggc tgtttctgaa tagtgtccct gcctttctaa gattctcgct
     3721 gcaccgagac aaccgtcagc atcccgcatt tgtgataatt tataataatt ttcaaatgcc
     3781 ttaccgtaat tcccctgttt ctcgtaaagc acggcaagat tgaaaagcac aactttattc
     3841 aaaggatcta acgcggtcgc cctgacaaga agactttcgc cgtccaaaag attgcccagg
     3901 gatatgtgcg caattccagc attgataagg gaggggatgt agttcatttt tatgccaaga
     3961 gccttgtttg cataggatag gctttcatca aacaacttca aacgtatata gattcctgca
     4021 atgttattca tggctacgta atttgctgga tccagctcaa gggctttcat gtagcaatca
     4081 atggcctgtc tcaattctcc ttttccttca tgagttcttg cagcgagaag gaaaacatct
     4141 ttattagttt tcccctcacc tttttccgcg ctctgtgttt caactgaaga cagttttaac
     4201 ccgacaggag cctgggtttt tgccatagcc tgaatgactg gttgcccctc cctcgtatgt
     4261 ttttctgcca ctttgggaaa gctatccatg gctggtgtca gtttttcttg ggaccccgtg
     4321 tgaggcagga ggatttttgg cacagcagtg acatgctgaa caggctcttt cacctcatca
     4381 aaagggttgc gtggtttgtt atcagaagcc actggagact gcggaggcag cctggcatct
     4441 gttttcgcag caatgcttac agacagggaa gacttgaaaa tgtccataac atagattgtt
     4501 ccaaatccgg ccagaaaaac aacgaggacc agtgtggaga gaaagataac ctttcgcctg
     4561 gttttcctct tattagcgcc atttatcacg gttttccgca aatctggtgg aatttccttt
     4621 ctcaagtctt ttgttttggt ttttgaaagt aaatctacaa gaaggctcat ctttctaatg
     4681 atctgtttcg ctgatttctc gtactgcaaa atgaagtgag gaagggccag acacagacga
     4741 ggatgtgccg ctcatatggt tttctggccg gctgagttcg gtgaataccc tccacgagta
     4801 ttttaccctt gccttaatac caagggcatt gtagcaacca atcgcctccc agtcatatcc
     4861 atgtttctcc acacattttt ttagtatctt tgcagcagtt ttgacattgt agcacgggtt
     4921 ttttaataac tcgctctcgt tgagccccat gggccttatc catgatgagt tgatctgcat
     4981 aatgccaaga tcagtcgagc catttcgatt ataacttaga gctgacgggt tgcagtttga
     5041 ctcaacagtt gcaatactcc ttaacaggct cgcattcaag ccgtactccc tggcagcttc
     5101 atcaaaacaa aaagcaaaca caaataaagg atggaatata aaaaggatag ctaatgccct
     5161 ttttcccaaa gtacttcatt gtagtagccc attctggaaa aatcaactgc tttttgaacc
     5221 attctccatg attattaaac tttcaataca aaaaaccgat aaacacaatt aacagattca
     5281 ttcaggcttg ttggaatttt taacgtctgg aatgaacggg ctaaaactgg aggtcctaaa
     5341 cgatgattat aacaaaaatg accaggaatc aaaaaggttt tacccttgtc gagttggcaa
     5401 ttgtactcgt tattattgga attattcttg gagcggtatt aaaggggcag gccctgatca
     5461 ataatgcaaa gattaaaagg gtctacaacc agcaaagaga ggtcctcgcc gctgtctatt
     5521 cttactttga cagatattcc ttcttccccg gtgatgatcc caactctgca acccgttggg
     5581 caggcgtgac cgccggcaat ggcaacggtc ttatagccgc aggtgcaggt tctactgcac
     5641 ctaactatgc atgtaccgca acaggaactg agcaatgcga tttgtggaat gaactccggc
     5701 tgtctgaatt catatctgga agcggattcc tgaatcctac aaatgcctat ggcggacgga
     5761 ttgcgttgac ctattattca gttcaggggc ttctcgcaca ctggattgcc tttcaaaaca
     5821 ttccttacga tgcatgtcaa tctattgatc tacagtttga tgacggagtc tacaacacag
     5881 gaggaatcag agggacagga aactatggca ctgcaacgag cggtgtcttt gatctttatt
     5941 ttaagttcta accccaaccc ccttacaatg gcaggaagga atggcacccc gccatattcc
     6001 ttcctgcccg ttctgcttat tctgccttta ttgttctttc agttgtacaa cctttcctga
     6061 aatccagcac tccctgccat cagaaatttt gactttgtgc cacacaatct cattttcccc
     6121 agctttttcc tctacaacct tgagtattgt tccctttgca acataagtta cgggagctga
     6181 atcgatcgat ggttcttgtc tcagaattgc tgaatgcaca gccacaacag caagtagcac
     6241 ttttggtttt gccgcttccg gttgtatctc tggtacaggt ttagtttctt ctgtggcctt
     6301 agaaagcttg acaggctttt caattttttg atccttatat tctaccgcaa tacggcgaaa
     6361 tccaaggcca ccggcaacac caataagtat ggtcaacaca acaatggcag cgtatttgct
     6421 gtaactcatc cagccatacg atctcttttc ccgctcagta agatgatcaa tagcgtatcg
     6481 tacgtggtgc ttctttatgt ctgtacttcc atccacatag gcagccatga gtgcacggga
     6541 tgaaaccaag ttaatcaacc gcggtacccc ttttgagtat ttaaatatga gattttttgc
     6601 gtcttcctca aaaacaggaa ggcctttccc agccttcatt gatcgatagt ttatataatc
     6661 ggaagtctct tcacgggtga ggggccttaa tgaagcctta accatcaccc gctgacttaa
     6721 ctgtttcata ttgtctgatg ccaatcgctc cctcaattct gtctgtccaa taagcactat
     6781 ctgaagcaat ttctcacgtt cagtctccag atttgaaaga aggcgcaact cttcgagggt
     6841 ctcatcagat agattctgtg cctcatcaac cacaattatc actgccttac cacgagagat
     6901 agaagaaaga agaaaatccc tgaaggcttt gagcatctca ttcttgtttt tgtctttgat
     6961 cgatactttc aagtcctcaa gaacaacatg gagaaattct tctggtgaaa gcctgggggt
     7021 aagcacaaga gctatttctg ccttgtgttt ccattcatca ataaatacct tcaggatcgt
     7081 tgtctttcct gtaccaggtt caccggtagc gagaaaaaaa ccctctttct gtgtgacagc
     7141 ataattcagc gcagtcaaaa tctcgttgtg ttcttttgac ggatagaaat aggtaaggtc
     7201 aggggtaatt gtaaaggggt cttctttcaa accaaaaaat tcaaggtaat ccatttacat
     7261 cttccctacc tttgatacaa ggtcatatac aggcaatagt actgccatga gaataagtat
     7321 gaaaacaagc ccaacaatgg aaaggagaat aggttcaatc attttgccaa gttttgcaga
     7381 aatatcgtca agccgtttaa tataataccc tgaaaggaaa gaaaactggg tgtcaaggtt
     7441 tccgctcttt tcaccggcac tgaccattct cgtcaccatg ggagggaata tattatggtc
     7501 cttgagagta tcagatatcc tgctgccaag gagtacattt tccctcactg caaccagggc
     7561 acgccgaaag acaagattat cgataacccc agcgacgata tcgagcgtcc tgtcgatggt
     7621 caaacctgct acaataagaa tcctcatttg ttcggcgaac agtgccaaga gtttattata
     7681 tacaatgagt ttcacaatcg gcagcttgag aagcatgtaa tcgtaataat agcgtgtttt
     7741 ctcccgggta cgcatgatct gaattgcgat tacacaaaga atcactatgc cgatgatcac
     7801 ataccagtag gttgaaatcg cattgctaac aaccatgagt gccctggtga tcaaaggcag
     7861 ttcaactccc atttcctgga ttacgttcat gatcttcggc agaacataga caaaccagaa
     7921 gatcattgcc cctgtagtgg ttacaattgc aaaaacagga tagaaaaggg agcgctttat
     7981 agcctggctc agatcttcaa ttctttgaag atgatctgct acatctgtta ggcttttgtc
     8041 taaccttccg gtagattcac caactttaac aagcctgatc agaatgtccg ggaagattcc
     8101 ccgttgtcgg gacagagcat cagagaaatt caatccgagt tctatttcct tcttagcatc
     8161 agtgagtgca gatttcagac ttctgttctc tgttgcctca atgctgtctt cgagggcgtt
     8221 taaaagaggt atacccgctg tcagcattac cgacaggttt cgaatacact cgaccatgtc
     8281 tccgcgttta acgcgtcgtg agataaagtg ttgtctgagc ctagcagcta tcttactggc
     8341 tttttttaca acaagaaccc tgaggttcct ggcggtcaaa tcttcgtacg ctgctgcctg
     8401 atcttcagcc tcaactatgc cgtcgatcaa tgacccatca atatttactg ctttgtagaa
     8461 atagtagttc atccggtaac cctgatgacc tcttcaagag tcgttacacc ttccgacgcc
     8521 tttctaaaac catcttctct gagaagaatc atgcccttgt tcagtgcagc agctctcatg
     8581 gctgtgatag gcgcctcgga aaaaatcagc tctctcattt catcatcgac aatcaatatc
     8641 tccccgatag ctgtccttcc tgaatatcct gtattgccac actttgcgca tccctgccct
     8701 ctgaacccct tctcagcaga cacaccgtat tcctggaact gggctcgttc ctcttcagtc
     8761 agagagtatt caactttaca gaatctgcaa atcttcctca acagtcgctg ggcaattatg
     8821 gcaagaagtg atgtggagag aagaaagcgg tcaactttaa gatccagtaa tcttgggatt
     8881 gaggtaactg cgtcgtttgt atgaagcgtg gagagcacaa gatgaccggt tatagatgct
     8941 ctgatcgcta tccgggcagt ctcttcatct ctcacctcac caagaagcat gacatcaggg
     9001 tcctgacgca taaaatttct tccagcaaga gcaaaatcat atcccacttt ttcgtttacc
     9061 tgggtctgcc tcacaaagct aagcttatat tccactggat cctcaacggt aattacgttc
     9121 ctttcaagaa ggttcacctc ccgtaaagct gagtaaaggg ttgtggtttt tccacttcct
     9181 gtcggccctg caatcagtac tattccgtaa ggcttttgaa aaagcaggcg aatcttttcc
     9241 gttcccttag cttcgaaacc aagtttgtcg atactcagga ggtaacctgt tccggcgagg
     9301 attctcatga ccacattctc accgtatatc gttggtacag tcgatgccct gatatcgtac
     9361 gatttatcaa gaaaggtaaa actgaacgag ccatcttgtg gaagccttgt ttcggcaatg
     9421 tctatttggg acattatttt tgttcgcgat acaatgcccc cgtgagcaac cttaggtaaa
     9481 gaaaaacgat gctgaaggac accatctatg cggtaaaaga ccgtggaaac atcgagaaaa
     9541 gggttgatgt ggatatcagt agccttttcc cgtatacctt ccataataat taattctgtc
     9601 atactggcta tttgttggcc agtggccatt cctgtcctta caacctcttt tactatttcc
     9661 tccatccttt cctgaatagg gttttcgagg aagaagtagc ttgtttcgag agaatcacgg
     9721 aaactgtctg gatccacaag gaacaccctg ggataattct ttgtgatatt aaccaccctg
     9781 tcaacagcag ttatattact tggatttgta acgccgatgg ataggagacc ttcatccaac
     9841 tcaatgggaa tgaactcttc cttttctgca atctccttgg gaatcaaccg cagcgcatcg
     9901 tctgataccg gatattcact caggtcaatg aactccattc caaactgttc tgccagtgtc
     9961 tgccctagct ctttagatgt tataaatccc aaccgtacga aaatatcgcc caggagatca
    10021 ccagttacct tctgatgaag aatcgaaata ttgatttgct tgtcgttgat cagacccttt
    10081 gttttaagta tgtctccaag tctgaatgta gccatagggt tacctcaatc tcagatcgct
    10141 cctcttaaag caatacaggc tgaaggatta ttacaagttc tgtgttaacc tctcccctgt
    10201 ccgagcttct gaaaagacca cccagaagtg gtaattttcc gagaaacggg gtctggttct
    10261 ctttcacccg ttcacttctc cgtgtaaggc ctccaataac aaccacctgt ccatccctga
    10321 cctttgcagt tgtgctcaac tcccgcaaat caacagtggg aagggatatc tgaagttgat
    10381 tggccatgct gccaatattc tttgtgtcaa gggtaataag ttctgatacg attggtgtta
    10441 tggtgaggga aatttctccc acatcatcga caaagggtac gatgcctatg gtaatacctg
    10501 aaagaatgct cgaggtgcca acagtaaagg taacaacagg gttcgttccc tggagggtgg
    10561 tgctttcaat ttgagaaata tatgcagtat tccttcctac tgttaacatc gctgtttgac
    10621 cattcatcat gttgatacgg gggttcgaaa gaagcctgac atcaccctgc tgttccaggg
    10681 catttaccag acctttaatg ttaattgaat ctatggtacc gtgtgaaagc gttctcgtta
    10741 tgccatcatc cccggtacca atactgaata ctggcgaggt tgaggacaca actgatgaga
    10801 agccggtgct tccaaggccg actgagccgc tccattttcc accaagacta aaagtaagat
    10861 tccagtcaat accaaactgg tacccctgtg acagggttac ttctatcacc tttgcctcaa
    10921 tgagaacctg cctggtcatc acctttctga ttctattaag gaattgtgca actttttcaa
    10981 gattattctt tgttgctgtt acaattattg tgcccgtaag cctgtttatt gtgaagtgct
    11041 catctgctgt tctgggacct ttcccaggcg gaccagatga gcctgcagta ccctgaccca
    11101 gaaggtttgc aattgattgt tccactgaat cccagaattt gaatgttgct tcatcgcttt
    11161 tcagagtctg tgagatagta cccttgatct ctgtggaagc tgttccagtt gtggctgctg
    11221 aggcgccacc gccagtagat acaggagcaa tattacccgt tacccctccg aagatattcc
    11281 caccgacctc aacattgtag ctctgtacca cgggagggat tccaatctca aaaacctttg
    11341 tgtcataagc cttaacaaac agcatagtat ctttgatcat atagaaataa ccggcagagg
    11401 aaaggagggt gtgcaaagca tcttcagcag aaacatttct caaagatata gttaccggtg
    11461 tttccgggac cacccctttc tccataacga cattcagatt cgttgcctct gctatcacat
    11521 ggagcacatc ttgcaaaggg gtgtttcggg caaccacatc cacaatcctc gtttttaaag
    11581 gagagatttc ctcggtcaca ggaatgaatt caggggcacg catctcaacc gtcctagaag
    11641 gttccgcctt cacctgcttt gtttcagacg gtatttcaat cgcctttttg actgtgtaag
    11701 aggaacaggc agtcatcagg aggaagaaaa aaatgaccac ggagtgacac aaaaaccgct
    11761 tcaaccttac cgtatcttta accactgctc tccctccctg ttttttatta acactccatt
    11821 tctttcaata cgggcaactc tgccctggct gattccatca ccctctctca caacaagatt
    11881 atcaatgatg gctattctct tctcgccacg aatcaaaaca agggaaacgt gttgctgttc
    11941 acgtggggca atatctgaaa gttttactga cggatagctc tttttctctg acaaatgttg
    12001 gtccatgggg tttttaaggg ctggcaccgc ggtgaaatgg cgttccttca tcataatttt
    12061 ctcctgcctg aataacaaaa tacccctttc aacagacgta agatg
//
LOCUS       DAOOMC010000085        11977 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdus sp. isolate AMR_MDS_2685
            NODE_5923_length_11977_cov_6.491025, whole genome shotgun sequence.
ACCESSION   DAOOMC010000085 DAOOMC010000000
VERSION     DAOOMC010000085.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864760
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdus sp. (wastewater metagenome)
  ORGANISM  Syntrophorhabdus sp.
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae; Syntrophorhabdus.
REFERENCE   1  (bases 1 to 11977)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11977)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,729
            Genes (coding)                    :: 2,708
            CDSs (with protein)               :: 2,708
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 7 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11977
                     /organism="Syntrophorhabdus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5923_length_11977_cov_6.491025"
                     /isolate="AMR_MDS_2685"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2699756"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            163..426
                     /locus_tag="PK125_09370"
     CDS             163..426
                     /locus_tag="PK125_09370"
                     /inference="COORDINATES: protein motif:HMM:NF014643.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system
                     prevent-host-death family antitoxin"
                     /protein_id="HPB38350.1"
                     /translation="MKIVGIRELKEKLSAYIDEVQTGKIILVTDRGKEVALLSPLSSE
                     FRLLRVLGKSGKARWAGGKPQGLNKEVIIEGKPLSSTILEERE"
     gene            423..890
                     /locus_tag="PK125_09375"
     CDS             423..890
                     /locus_tag="PK125_09375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HPB38351.1"
                     /translation="MILYLGASSLVKLYVQEPDSGLVRAWIRSAEYVATCRIAYTEVI
                     SALSVRYRKGDLSKNDYDLIAGTFIKDWESYIKVDFDDLEAGNLAMKYDLPRLSAMHL
                     SAALFLASEYGKCKSASFSEIKSRPETAVFFSSADPSLSQAAQNEGLTVLSLR"
     gene            913..2121
                     /locus_tag="PK125_09380"
     CDS             913..2121
                     /locus_tag="PK125_09380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB38352.1"
                     /translation="MSDFYQTGVVATFHRLNAENQTTIETKLEQYAEERPIALVLPSL
                     FSELKGQALQNIVGELKKVNYIKEVVVTLGPASEKEFIEAKSFFSVLPQKTRVIWNNG
                     KRMRKIYKSIESEGLPTGLEGKGRSAWMAYGYVLSQHQFNAIALHDCDIVTYTKDMLT
                     RLCYPVTNPGMDYDFCKGFYARVTDKMHGRATRLLVTPLIRALQKILGHHPLLAFFDS
                     FRYILAGEFSMDVNLARINRIPGDWGLEVGTLAEVYRNTSISRVCQIDIADNYEHKHQ
                     ALSPEDASKGLHKMSVDICKSVFRTLASEGVIFSDSFFKTLMTTYARTAQDMLKRYED
                     DASVNGLLFDRHEESLAVEIFTQGLKRSSEVIVKDPLGVPLIAGWDRVFAAIPDILDT
                     IREAVEADNR"
     gene            2162..2824
                     /locus_tag="PK125_09385"
     CDS             2162..2824
                     /locus_tag="PK125_09385"
                     /inference="COORDINATES: protein motif:HMM:NF039842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5752 family protein"
                     /protein_id="HPB38353.1"
                     /translation="MDEIKPFEFMECVTILKATGKKARNIHDLRALISAISNESLFHH
                     TYQYFLKGHILEYTNDFAHWVGESLEERALSERLSNIDPFAFKDIPSLRQEFLDEIDD
                     YIRNFPKPRNAMSGDEFYFNETVTLIFPVGVQAHNLAEFLLAIRYVDQACIYYHFYEA
                     RIRHGGDDFSAWIETVLGNPGLARSIRAIDPFMHNIEGIRTHIVDAVDNDLRCAMEVI
                     KP"
     gene            2821..4041
                     /locus_tag="PK125_09390"
     CDS             2821..4041
                     /locus_tag="PK125_09390"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HPB38354.1"
                     /translation="MISRYAGVSPKGDLILLKRLGDKMGGKSFLHVNSTKAGGGVAEI
                     LHRMMPILQGLGIRARWEVIEGDEQFFDITKKIHNALQGNREIITKKMWQHHFDVNEK
                     NMATIQLDEVDAALIHDPQPAPLIEFKKSGKWIWRCHIDVSNPMKEVRDHLFQYCKKY
                     DAAVFSVARFAKAMGIDEFIIPPSIDPLSDKNRDLTEEEIRETAEKLSLPLDRPIILQ
                     VSRFDRFKDPTGVIEAYRMVKRYNDCVLVLAGSPATDDPEGAAVLALVKEFASSDPDI
                     HILLLPPFSDKDINALQRMATVVLQKSLKEGFGLTVTEAMWKGKPVIGGAVGGIPLQV
                     IHGVTGFLVHSVEGAAFRIRQFLNNPEMAKRMGERGKEHIRQHFLITRQIRDYLSIWY
                     TLERGEDGTSGLSI"
     gene            4038..6290
                     /locus_tag="PK125_09395"
     CDS             4038..6290
                     /locus_tag="PK125_09395"
                     /inference="COORDINATES: protein motif:HMM:NF013173.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-6-phosphate synthase"
                     /protein_id="HPB38355.1"
                     /translation="MKITIRLIVSLVLVVALAVFAFSLYQVNYEKSRLARDLERRSII
                     LAESLQESIAPLIQSNAVGKLNRIVERFGNRERLKGIAVFDKQGNALTLTAHLRSQIT
                     QPFPQAVNSVAENRSIGSFMTIGEQKMYIYVIPIYSENAEIAGALTTFNDTSYINTRL
                     REIWKDNVLRFFTLSLLVVLSTILVVRWSITGPIARLAEWMREFRTSKEERENPVVPI
                     RGDILTPLISEVTQLAKSLSMARAKAEEEVRSRSKSDSVWTADSLKEFMKVELEDQKL
                     FVVSNREPYMHVKEGRATKCIVPAGGLVTALDPVMRICDGIWLAHGSGDADREFVDAY
                     NKVRVPPEEPAYTLKRVWLTKEQEDGYYYGFSNEGIWPLCHITHTRPIFRLEDWICYQ
                     KVNEFFAESLMEEITMEESPLVLIQDYHLALLPLLIKQRRPDAKVALFWHIPWPNPES
                     FGICPWRQEILIGMLGADLLSFHIQFFCNNFLDTVDRFLESQIDWEQFSVKRSGHTTL
                     VKPFPISVSYDQKKETEATSNDNTSIREYIMKEIGIQVKYLGVGVDRIDYTKGIPERF
                     RAIERFLEKYPEFVEEFTFIELGAPSRDHIRKYRELMSEVEETVEKINWRYQTKTWKP
                     IVYLKAHHSHEEIERYYRASNICMVTSLHDGMNLVAKEFIISRHDNDGVLILSQFAGA
                     SRELKDSIIVNPYDIEEMADSIKFALTLEPSERAERMQRMRNMVKEHNIYRWAGKLIA
                     ELSHVRLAGE"
     gene            6318..7118
                     /gene="otsB"
                     /locus_tag="PK125_09400"
     CDS             6318..7118
                     /gene="otsB"
                     /locus_tag="PK125_09400"
                     /EC_number="3.1.3.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004805 - trehalose-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-phosphatase"
                     /protein_id="HPB38356.1"
                     /translation="MYPKYFFENGDPRLKDNLSELFLFLDFDGTLVPIKDDPAQCFLK
                     SDTMSQMEAISQSGKATVAVLSGRTVEEIKKRVPVQGIYYGGNHGLEISGPDLSYLHP
                     DALFGKQAMEQIFKKIKKKTLNIGGALVERKKFGFSLHYRMANKEDRVLIKKIFYETI
                     SSNQYRYTFSILPGKKVLELVPKVKWDKGRAALFLLKRQKKDYLPIYVGDDKTDETGF
                     NALRTQGVTVRVGKSKKTKAEYYLKGQWQMIKFLQHIHSLVKLGNRYA"
     gene            7111..8385
                     /locus_tag="PK125_09405"
     CDS             7111..8385
                     /locus_tag="PK125_09405"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HPB38357.1"
                     /translation="MHSVIWPDSLGDYIKSIKSADILVGIPSYNNAKTIGHVVRAVQG
                     GFAKYFPEKKTVLVNSDGGSTDGTMEVVQDATIDSLQSILLSHKVNPFVKIATPYHGI
                     PGKGSAFRRIFEIADYLQIKACAVVDSDLRSITPEWIELLIKPVLEGEFDYVAPLYQR
                     HKYDGTITNSIVYPLTRALYGKRVRQPIGGDFGFSGRLAKYFLSEDVWETDVARYGID
                     IWMTTTAIANNFKICQSYLGAKIHDPKDPGADLSAMLYQVVGSTFDLMDTYSDVWKNV
                     SKSEPVPTFGFRYDVGLEPVTVNLERMIDRFCLGVQELGFVWKDILPRSLFNFFKDIS
                     TASCPQDFIIPDDVWVDTIFSFALATHRKVINREHLLRSLTPLYIGRVASFVVDTINS
                     NAVEVEEKIEVLCTAFERKKTVLFSEWDRKGV"
     gene            8388..9083
                     /locus_tag="PK125_09410"
     CDS             8388..9083
                     /locus_tag="PK125_09410"
                     /inference="COORDINATES: protein motif:HMM:NF017373.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB38358.1"
                     /translation="MNLIERIMVEPFEKFYEKLLVFLPNLFTSLILLVFGFLLGFFMK
                     WIFMKIFRAINLDGLSQKMGMKDALGRSGFREPLSLILSRILKWFTIITFIIVAMQNL
                     SIPTVEHLIDRMFLYVPNIFTAALILVLGYILGNFFGRAAIIASVNADMKSAGLIGKS
                     VKFIVILLSCTMALEQLGIGKDTIVIAFAIVFGGIIFAFSLAFGLGGKDIAKGYMDRK
                     LKKESNEDEMDHL"
     gene            9146..9328
                     /locus_tag="PK125_09415"
     CDS             9146..9328
                     /locus_tag="PK125_09415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB38359.1"
                     /translation="MLGFSDTWVFIGYALTVCCTLFCLAYGIINWRKGITEKDGDYRE
                     EIRWEKEEIELIEKLP"
     gene            9331..10911
                     /locus_tag="PK125_09420"
     CDS             9331..10911
                     /locus_tag="PK125_09420"
                     /inference="COORDINATES: protein motif:HMM:NF012686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HPB38360.1"
                     /translation="MFYLITFTFVYLAITVYFSYLGYKKTTTVSDYLLAGRQVHPVVM
                     ALSYGSTYISTSAVIGFGGISALYGFSLTWLTFLNILVGVWIAFVVFGKRTRKMGKAL
                     DAHTFPELLGRRYESRFIQGFSGFVILVFMPLYTAAVLIGISRFIEVYMKIPFSTAFL
                     AFLLITACYVIWGGLKGVLYTSAFQGVIMVVVMIGIGVSTYSSVGGIVPGHAALSKLT
                     YLLPENLKAMGHSGFTSMPTGGSSLWWYVITTMIMGVGIGVIGQPQLSVRFMTLKSDR
                     ELNRSIPFTALFIIFTTGIAFAVGALTNVFFYGRFGQLSIQAVSGNVDKIIPLFIDIF
                     YPQWFVALFLVTLMAAAMSANSSQFHAIGTSLSRDIFEQALLRGRSVAETTIVTRVGI
                     VFSIFATLIIGLLLPESIVAIATAFFFSLCGATFIPAYLFALYWKRATKTGAKASILC
                     GFFLSLLWMLLVHEQESKAIGLARYLFSSDSLLGHPWNMIDPQVIALPVSFVVFCAVS
                     YFSQPVKTETIESAFRHI"
     gene            11082..11357
                     /gene="rpsT"
                     /locus_tag="PK125_09425"
     CDS             11082..11357
                     /gene="rpsT"
                     /locus_tag="PK125_09425"
                     /inference="COORDINATES: protein motif:HMM:TIGR00029.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HPB38361.1"
                     /translation="MRKNKSAIKRARQSEDRRLRNSHIKSTMKTYIKRVVTALENKEK
                     ESLDAVLKNTVSYINKAASQGVVHKNNAARKVSRLSKKVHSFLHGKA"
     gene            complement(11359..11865)
                     /gene="lptE"
                     /locus_tag="PK125_09430"
     CDS             complement(11359..11865)
                     /gene="lptE"
                     /locus_tag="PK125_09430"
                     /inference="COORDINATES: protein motif:HMM:NF016290.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HPB38362.1"
                     /translation="MKSIIYLLITIIMATLLPGCGYELVRDKGIFGGEITTLSVPTFK
                     NTTYEPHVSKYVSQAFTRELMGTGVFRLDRTNTDGYIEGTIRDIRTIPNTLSKTGIVT
                     EKKMYMDVELILYRKNGTFIRKWMFSDNEVYRADVPNYEEYNKRTAYERLSERMARRF
                     SAAILVNY"
BASE COUNT         3408 a         2444 c         2796 g         3329 t
ORIGIN      
        1 acgttcacta ccttaaatat ataccacatt tattggtaat gaagtctcat attaatattt
       61 gaaaggttat tttaatatat aatttgtgac tacattatta cttgacatgt aactacttaa
      121 ttattattat ttactattgt tcaataaaac ggagatatga aaatgaaaat agttggtatc
      181 agggagctca aagaaaaact gagtgcttat atcgatgagg tccagacagg caagatcatc
      241 cttgtaaccg atagggggaa agaggttgca cttttgagtc ctctatcaag tgaattccgg
      301 ttgttaagag tccttggaaa atccgggaag gcgaggtggg ctggaggaaa acctcagggg
      361 cttaacaaag aggttatcat tgaaggtaag cctctttctt caaccatact ggaggagcgg
      421 gaatgattct ttatctgggg gccagtagcc tggtaaagtt gtatgtacag gagccagatt
      481 caggcttggt gagagcctgg ataaggtctg cagaatatgt ggcgacatgc cgtattgcat
      541 atacagaagt aataagtgcg ctgagcgtac gatacaggaa gggggacctt tcgaagaacg
      601 actatgatct cattgcgggt acattcataa aagactggga gagttacatt aaggttgatt
      661 tcgatgacct tgaagcaggg aacctggcca tgaaatatga cctgcctcgc ttgagcgcta
      721 tgcatttgtc ggctgcccta tttctcgcaa gtgaatacgg gaagtgtaaa agtgcatctt
      781 tttcagaaat aaagagtcga cccgaaactg ctgtcttctt ttcttctgct gatccttcgc
      841 tcagccaggc tgcccagaac gaagggctaa cagtgttgtc cctcagataa ctaaaagatt
      901 aaggaggtta cagtgtccga tttttatcag accggagtgg tagcaacctt tcaccggctt
      961 aatgcggaaa atcagacaac aattgaaacc aagctcgaac aatacgccga ggaacggccc
     1021 attgctcttg tgctgccttc tttgttcagc gagctaaaag gtcaggccct ccagaatatt
     1081 gttggggagc tgaaaaaagt aaactacata aaggaggttg tagttactct tgggcctgca
     1141 tctgaaaagg aattcattga agcaaaatcg tttttttccg ttcttcctca aaaaacaagg
     1201 gttatatgga acaatggtaa acggatgaga aaaatttaca agtctattga atcagaggga
     1261 ttgcccaccg gtctggaggg aaaaggaaga tcagcatgga tggcctacgg ctatgtcttg
     1321 tctcagcatc agttcaacgc catagccctt catgactgtg acatagtcac atacacgaaa
     1381 gatatgctta ctcgtctttg ttaccctgtc accaatccag gtatggatta tgatttctgt
     1441 aaaggatttt acgcgagagt aactgacaag atgcatggaa gggccacaag gctccttgtt
     1501 acaccgctta taagggctct tcaaaagatt ctcggccacc atccgttgct ggctttcttt
     1561 gacagtttcc gttatatcct ggccggtgag ttttccatgg atgtgaacct tgcaagaatc
     1621 aacaggattc ccggggactg gggcctggaa gtgggaacac tcgcagaggt ctacagaaac
     1681 acttccataa gcagggtctg tcagattgat attgccgaca attacgaaca taaacatcag
     1741 gcactttctc ctgaagatgc ttcaaaaggc ctgcacaaaa tgtctgtcga tatctgtaaa
     1801 tctgttttta gaacccttgc ctcagagggt gttatctttt ccgacagctt cttcaagacc
     1861 ttgatgacaa catacgcaag gactgcccag gatatgttga aaagatatga agacgacgct
     1921 tctgttaacg gactcctgtt tgaccgccac gaggagagcc tggcagtgga aatttttacg
     1981 cagggattaa aaaggtcctc agaagttatt gtgaaggatc cccttggagt tcctcttata
     2041 gcaggctggg atagggtgtt tgcagccata ccagacatac tcgatacaat cagggaggct
     2101 gttgaagcgg acaacaggta aaaatagggg cttgttctaa atacctgtga gtggaggaac
     2161 aatggacgaa ataaagcctt ttgaatttat ggaatgtgta accatcttga aggccacagg
     2221 aaagaaggct cgaaacatcc atgacctgag ggcattaata tcagccatta gcaatgagag
     2281 cttgttccat cacacctatc agtatttcct gaaagggcat attcttgaat atacaaacga
     2341 ctttgcccat tgggtgggtg aaagccttga ggaaagggct ctttctgaac gcctctccaa
     2401 tatcgaccct tttgctttta aggatatacc gagcctcagg caagaatttc ttgatgagat
     2461 agatgattac atcagaaact tccccaagcc aagaaatgct atgtcaggag atgagtttta
     2521 cttcaacgaa actgtcaccc taatctttcc tgtcggagta caggcacata accttgccga
     2581 gttcctgctt gcaatcagat acgttgacca ggcctgcata tattatcact tctatgaggc
     2641 acgaattcgc cacggtggtg acgatttttc agcatggatc gaaacagtgt tgggtaatcc
     2701 tgggttagcc aggtctatcc gggccatcga tccctttatg cacaacattg aagggatacg
     2761 aacccatatt gtagatgccg ttgacaatga cttgaggtgt gccatggagg tgataaagcc
     2821 atgataagta gatatgccgg ggtatcacct aagggagacc ttattctatt aaaaagactt
     2881 ggtgacaaga tgggtgggaa gtcctttctt catgtcaatt caacaaaggc agggggagga
     2941 gtagcagaga tactacaccg tatgatgcct atcctccagg gactcggcat tagagcgcga
     3001 tgggaagtta tcgaaggtga tgagcaattc ttcgatatta ccaaaaagat tcataatgcc
     3061 ctccagggca acagagagat tatcaccaag aagatgtggc aacaccattt tgacgtgaat
     3121 gaaaaaaata tggcaacaat acagcttgat gaagttgatg ctgccttaat acacgatccc
     3181 cagcctgccc ccctgatcga attcaagaag tcaggcaaat ggatttggcg ctgccacatt
     3241 gatgtatcaa acccaatgaa agaagtccgt gatcatttgt ttcaatactg taaaaaatat
     3301 gatgccgcag tcttttcagt tgccagattt gcaaaagcaa tgggcattga tgaattcatt
     3361 atacctccat ctatcgaccc cttgagtgac aagaacaggg atctcaccga agaggagatc
     3421 agggaaactg ccgaaaaatt aagtttaccc cttgacagac ccataatcct ccaggtttcc
     3481 cgttttgaca gatttaagga tcctactggg gttatcgagg cataccgcat ggtaaaacga
     3541 tacaatgact gtgttctggt tctcgcaggc agtcctgcaa cggacgaccc cgagggggca
     3601 gcagtgctgg cgctggtgaa ggaatttgct tcttctgatc ccgatatcca cattttgctg
     3661 ctgcccccgt tcagcgacaa ggatataaac gctctgcaga ggatggccac cgttgtgctc
     3721 cagaaatcgc tcaaagaagg atttggcctt actgtcacag aggctatgtg gaaaggcaag
     3781 cctgtgatcg gaggcgctgt aggaggaata ccattacagg taatacatgg ggtaaccggt
     3841 tttcttgttc actctgtgga aggtgccgca ttcaggataa gacagtttct taataaccct
     3901 gagatggcaa agagaatggg cgagagagga aaggaacata tacgacagca ttttcttata
     3961 acgaggcaga taagggacta cctgtctatc tggtatacat tggaaagggg agaggatggt
     4021 acttcaggat tgagtatatg aagataacta ttcgtctcat cgtttccctt gttctggttg
     4081 tagcactcgc agtttttgct ttttccctgt accaggttaa ctatgagaag agccgtctgg
     4141 caagggatct tgaacggcga tccataattc ttgctgaaag tttgcaggag tctattgcac
     4201 cgcttataca gtcaaatgct gttggaaagt taaaccgtat tgtggaaaga tttggaaatc
     4261 gagaacgact gaaagggatc gcggtatttg acaaacaggg aaacgccctc accttaactg
     4321 cccatctgag gtcccaaatt actcagcctt tcccacaggc agtaaactca gtggctgaaa
     4381 accgttccat aggaagcttt atgactatcg gggaacagaa gatgtatatc tatgtaattc
     4441 caatttacag tgaaaacgct gaaattgcag gggcgctgac cacgttcaac gatacttctt
     4501 acatcaacac acgtctccgt gaaatatgga aagacaatgt cctccgtttt ttcacccttt
     4561 ccctgctggt tgtgctcagt actatactgg ttgtcagatg gagcataaca ggaccaatag
     4621 cacgcctggc cgagtggatg agagagttca gaacttcaaa agaagaaaga gaaaaccctg
     4681 tagttccgat acggggtgat atattaaccc ctcttatatc cgaggtaacc cagctggcga
     4741 agagtctctc catggcccgt gcaaaggcgg aagaagaggt aagatcacgt tcaaagtcag
     4801 attctgtttg gaccgcagac agtctgaagg aatttatgaa ggtggaactg gaggatcaaa
     4861 agctgtttgt ggtctccaac agagaaccat atatgcatgt aaaagagggg cgggccacaa
     4921 aatgtattgt gccggctggt ggccttgtga ccgcccttga tccggttatg aggatatgcg
     4981 acggcatttg gttggctcat ggaagtggtg atgctgatcg agagtttgtc gatgcttaca
     5041 ataaagtgcg tgttcctccg gaagaaccag cttacaccct caaacgcgta tggcttacca
     5101 aggagcaaga ggatggctac tattatgggt tttcgaatga aggcatctgg ccattatgtc
     5161 atatcaccca tacacgtcct atttttcgct tagaagattg gatttgttat cagaaagtca
     5221 atgagttttt tgcagaatca cttatggagg agataaccat ggaagaatct cctcttgtgt
     5281 tgatccagga ctatcatctt gcccttctcc ctcttctgat aaagcagagg aggcctgatg
     5341 caaaggtcgc acttttctgg cacatcccct ggccaaaccc agaatccttt ggaatctgcc
     5401 cctggcgtca ggagattctt atcggcatgc tcggggcaga cctgttaagc tttcacatac
     5461 aatttttctg caataatttc ctcgacactg tggacaggtt tctcgaatct cagatagact
     5521 gggagcaatt ctctgtcaag cgaagcggac acacaacgct ggtaaaacca ttcccaataa
     5581 gtgtcagtta tgatcaaaaa aaagaaactg aagccacaag caatgacaat acatctatac
     5641 gtgagtacat catgaaggaa attggtattc aggtaaaata cctgggtgtt ggtgtggatc
     5701 gcatcgatta cacgaaaggt attccggagc gtttcagggc aattgagcga ttcttagaaa
     5761 aataccctga atttgttgag gaatttacat ttattgagct tggtgcgccc agccgtgacc
     5821 atattcgaaa atacagggaa ttgatgtccg aagtggaaga aacagtagaa aaaataaact
     5881 ggagatatca gaccaaaaca tggaagccca ttgtatatct gaaagcccac cacagccatg
     5941 aggagattga acgttactat cgggcgtcaa atatttgcat ggtaacctct ttacatgacg
     6001 gcatgaacct cgtggcaaag gagtttatca tttcacgtca cgataatgat ggtgtattaa
     6061 tactgagcca gtttgcaggg gcatccaggg aactcaaaga ttcaataatt gtaaacccat
     6121 atgatataga ggaaatggca gattcaataa agtttgctct cacccttgaa ccttctgaga
     6181 gggctgagcg tatgcaaaga atgcgtaaca tggtaaaaga acacaatatc taccgttggg
     6241 cagggaaact gattgctgaa ctgtcacatg tgcgcctggc cggagaataa tggcatacat
     6301 cctgaaaggg agaagcaatg tatcccaaat atttttttga aaacggcgac ccaaggttaa
     6361 aggataattt gtccgaatta tttctctttc ttgattttga tggcaccctg gtacccataa
     6421 aagatgatcc tgcacaatgt tttttaaaat ccgatacgat gagccagatg gaggcaatct
     6481 ctcagtctgg aaaggcaact gtggccgtat taagtggtag gactgttgag gaaatcaaga
     6541 aaagagtacc ggtacaaggt atttattacg gaggaaacca tggcctggaa atttccggac
     6601 cagacctttc atatcttcat cctgatgccc ttttcgggaa acaggctatg gagcagattt
     6661 ttaaaaagat caaaaaaaag acgctgaaca taggaggagc actggttgaa agaaagaagt
     6721 ttggcttctc cctccactac aggatggcaa acaaggaaga cagggtattg ataaaaaaga
     6781 ttttttatga gaccatctcc agtaaccagt atcgatatac cttttctata ttgcccggga
     6841 aaaaagtgct cgaattagtt cccaaggtta aatgggacaa aggcagggca gccttgtttc
     6901 tcttaaagag gcagaaaaaa gactatcttc ccatttatgt tggagatgat aagaccgacg
     6961 aaacaggttt taatgcatta cggacgcagg gggtaactgt cagggtgggg aaatctaaga
     7021 aaactaaagc agaatattat ctcaagggtc aatggcaaat gataaaattt ttacaacaca
     7081 tacattcctt ggtgaagtta ggaaacaggt atgcatagcg ttatttggcc ggacagtctt
     7141 ggggattaca taaaatctat aaaaagcgcc gacattctgg ttggaattcc cagttacaac
     7201 aacgccaaaa caatcggaca tgttgtaagg gctgtacagg gaggattcgc caaatacttc
     7261 cctgaaaaga agactgtact tgtcaattca gatggtggat caacagacgg aaccatggag
     7321 gttgtgcagg atgcaacgat tgatagcctt cagtccattc ttttatctca caaggtaaat
     7381 ccttttgtaa agattgcaac cccctatcat ggaattccgg ggaagggaag tgctttccgt
     7441 agaatctttg aaatagcaga ttatttgcag ataaaagcgt gtgccgtggt tgactctgat
     7501 ttgcgaagca taacccccga gtggatagaa ctgctcatca agcctgtcct ggagggtgaa
     7561 tttgattacg tggcgccgct ctaccagaga cataagtacg acgggactat aacgaatagc
     7621 attgtttacc cattaacgag ggccctttat ggaaaacggg tacgacagcc catagggggg
     7681 gattttgggt tctcaggtcg tctggccaaa tacttcctat cagaagatgt atgggagacg
     7741 gatgtcgcca gatacggaat agacatatgg atgacgacca cagccatcgc aaataatttt
     7801 aagatctgcc aatcatatct gggagcaaaa atccatgacc caaaagatcc aggagctgac
     7861 ctgagtgcaa tgctttatca ggtagttgga tcaaccttcg accttatgga tacgtattca
     7921 gatgtttgga aaaatgtcag caagtctgaa ccagtgccaa cctttggttt tcgttatgat
     7981 gttgggctag aacctgtaac cgtgaatctg gaacggatga ttgacaggtt ttgccttggc
     8041 gttcaggaac tcggctttgt ttggaaggat attcttcctc gaagtctttt caatttcttc
     8101 aaggatataa gtacagcttc ctgtccgcag gattttataa tacccgatga cgtctgggtc
     8161 gatacgatat tcagctttgc cctggcaacc cacagaaagg ttattaacag agagcacctg
     8221 ttacgctctc ttacgccgtt atacattggc agagttgcat cttttgtcgt ggacactata
     8281 aatagcaatg cggtcgaggt tgaggaaaag atagaagtgc tgtgcacggc ttttgaaaga
     8341 aagaaaacag ttctttttag tgaatgggac cgaaaggggg tataggaatg aatcttattg
     8401 aaaggattat ggttgagccc tttgaaaaat tttacgagaa gcttcttgtc tttctcccga
     8461 atcttttcac ctccctcata ctgcttgtct ttggttttct tctgggtttt tttatgaaat
     8521 ggatttttat gaaaattttc cgggccataa accttgatgg gttatcgcaa aaaatgggca
     8581 tgaaagatgc ccttggaaga agtggcttca gagaacccct gtcgcttatc ctttccagaa
     8641 tcctgaaatg gttcacaata attaccttca ttattgtggc catgcagaat ttgagtatac
     8701 cgacggtaga acatctgatt gacaggatgt ttctctatgt acccaatatc ttcactgcag
     8761 ccctcattct cgtcctgggg tatattctgg gcaatttttt tggaagggct gcaatcatag
     8821 cttctgtcaa tgctgatatg aaaagcgcag gtttgatcgg aaaatcagta aaatttattg
     8881 tgatccttct ttcgtgtacc atggcgttag aacagcttgg aatcggcaaa gatacaatcg
     8941 tcatcgcttt cgcgattgtc tttggaggaa ttatatttgc attttctctt gcctttggtt
     9001 taggaggaaa ggacattgca aagggttaca tggatagaaa attgaaaaaa gagagtaatg
     9061 aagacgaaat ggatcatctt tgaacgctgt tgttggggta ttaaaacctt gacattttta
     9121 ggacctgcat gtcattaata ctatcatgct tggattcagc gatacctggg tttttatcgg
     9181 atacgccctc acagtctgtt gcaccctttt ctgccttgcc tatggtatta taaactggcg
     9241 caaaggtatt accgagaagg acggcgatta cagggaagaa atacgctggg aaaaagaaga
     9301 aattgaatta atcgaaaaac ttccttaata atgttttatc tgatcacctt tacctttgtg
     9361 tatcttgcta ttacagtcta ttttagctat ctaggctaca agaagaccac aaccgtttct
     9421 gattatctcc tcgcaggcag gcaagtacat cctgttgtca tggctttaag ttatgggtca
     9481 acctacatta gtacttctgc tgttattggt tttggaggca tcagcgcatt atatggcttt
     9541 agcctcacct ggcttacctt cctcaatatt cttgtaggtg tttggatcgc ctttgttgtt
     9601 tttgggaaaa ggacgagaaa aatgggcaag gccctcgacg cccatacctt ccctgaacta
     9661 ctgggaagaa ggtacgaatc acggttcata caggggtttt ccggttttgt tattcttgta
     9721 ttcatgcctc tgtacacagc agcagtctta atcggaattt cccggttcat tgaagtctac
     9781 atgaaaattc ccttctctac tgcttttctt gcctttcttc tcattactgc ctgttatgtt
     9841 atatggggtg ggctgaaagg ggtgctgtac acatcagcct ttcagggcgt aatcatggtg
     9901 gtggtgatga tagggatagg tgtgtcaacc tatagctcgg tagggggcat agttcctggt
     9961 catgctgcgc tttcaaagct aacatatctt ttgccggaaa accttaaagc aatggggcat
    10021 tcaggcttta cctcgatgcc caccggtggt tcgtcccttt ggtggtacgt aattaccaca
    10081 atgataatgg gtgtgggtat cggtgtcatt ggccaacccc agctcagtgt ccggtttatg
    10141 actttgaaat cagacagaga acttaacaga tccattccct ttaccgccct ttttatcatc
    10201 tttaccacag gcatcgcatt tgctgttggg gccctcacca atgtcttttt ttatggaaga
    10261 tttggacaat tgtcaattca ggcagtatcc ggaaatgttg ataaaatcat accgcttttt
    10321 atcgacattt tttatcccca gtggtttgtg gcactttttc tcgtaaccct tatggctgct
    10381 gccatgagtg ccaacagcag ccagtttcat gcaattggca catccctgag cagagacatc
    10441 ttcgagcagg ctttgttgag aggcagatca gtggcggaaa ccaccattgt cactcgggtc
    10501 ggtattgttt ttagcatttt cgcaacgttg ataattgggc ttctccttcc ggaaagcatt
    10561 gttgccattg ctactgcatt tttcttcagc ctgtgtggag caacttttat accggcatat
    10621 ctttttgcgc tttactggaa aagggctacc aagacaggcg ctaaggcaag tatcctctgc
    10681 ggcttctttc tttcgcttct ctggatgctt ctcgtccatg agcaggaatc aaaagctatc
    10741 ggattagccc ggtatctctt tagctctgat agccttttgg gccatccctg gaacatgatt
    10801 gatccacagg ttattgcatt accagtctct tttgttgttt tttgtgcagt ctcttatttt
    10861 agccagcctg taaagactga gactatcgag agcgcatttc gacatattta gaaagtatgg
    10921 gaagcacagc gctaagagca attggtgcat agacctatcg gctcagagct atgagtttcc
    10981 tttcccatat ccttttgcct atgacagggt ttcttttgat tcttattgac ttcaattgca
    11041 gattaatggt aaataaacca aaatttacag gaggatagat tttgaggaag aacaaatcgg
    11101 caataaaaag agcaaggcag tcagaggaca gaagattaag aaattctcat attaaatcga
    11161 ccatgaagac ctatattaaa agggtggtaa ctgccttgga aaacaaagag aaggaatctc
    11221 tggatgcagt tttgaaaaat actgtttcat atattaacaa ggcagcatcc cagggtgtgg
    11281 ttcataagaa taatgcagca agaaaagtct caagactctc aaaaaaggtg cacagctttc
    11341 tgcacgggaa agcttgatct aataattcac aaggatggcc gcggaaaacc tgcgggccat
    11401 ccgctccgat agcctctcat aagcagttct cttgttgtac tcctcataat tgggaacatc
    11461 tgccctgtat acctcattgt cagaaaacat ccactttctg atgaaggtcc cgtttttcct
    11521 gtacagaatg agttccacgt ccatgtacat cttcttttct gtgacaatgc ctgttttgct
    11581 caaggtattt ggtatggttc ggatatccct tatagtgcct tcaatataac cgtccgtgtt
    11641 tgtcctatca agcctgaaga ctcctgtgcc catgagttct ctcgtaaatg cctgggaaac
    11701 atactttgaa acatgtggtt cataggtggt atttttaaag gtgggcacag agagggtggt
    11761 aatctctcca ccaaaaatcc ccttatcccg gaccaattca tatccgcacc ctgggagcag
    11821 cgtagccata atgatggtta ttagcaaata aattattgat ttcataatat gtaatctctt
    11881 ttgtttacgt gcatgccctg tttttgtgca taagcagata gttcatggtt cacaacagaa
    11941 cgattaaaaa acaaaggcaa actgggtatt acttaac
//
LOCUS       DAOOMC010000086        11961 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdus sp. isolate AMR_MDS_2685
            NODE_5933_length_11961_cov_5.938854, whole genome shotgun sequence.
ACCESSION   DAOOMC010000086 DAOOMC010000000
VERSION     DAOOMC010000086.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864760
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdus sp. (wastewater metagenome)
  ORGANISM  Syntrophorhabdus sp.
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae; Syntrophorhabdus.
REFERENCE   1  (bases 1 to 11961)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11961)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,729
            Genes (coding)                    :: 2,708
            CDSs (with protein)               :: 2,708
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 7 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11961
                     /organism="Syntrophorhabdus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5933_length_11961_cov_5.938854"
                     /isolate="AMR_MDS_2685"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2699756"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..307
                     /locus_tag="PK125_09435"
     CDS             <1..307
                     /locus_tag="PK125_09435"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HPB38363.1"
                     /translation="LRKSDTIARMGGDEFLVIVPETERPEDVATVAQKILGVFQIPFD
                     CNSFKQSSSTSIGVAMYPKDGDNSEVLIRCADIAMYSAKAKGGNSFCIYTPEIESKQ"
     gene            510..1232
                     /locus_tag="PK125_09440"
     CDS             510..1232
                     /locus_tag="PK125_09440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0280 family protein"
                     /protein_id="HPB38364.1"
                     /translation="MYEERFYRNIARPADLLCYGVTYKETDLWCCTKTDLRKVIEDRV
                     FYYRHQLEEYIRTNPIFNESLLPINHDQFAPVIVKEMISASARVGVGPMATVAGAIAE
                     FLGRDIAHLTDEYIIENGGDISIKTLRERMILVYAKKSPFSQNIAIRIKERDKPYGVC
                     TSSATVGHSLSLGKADAVCVIADSALFADGLATYIGNSVKSKDDIGGAIEAGKTFSNV
                     MGLLIIVGDSLGVWGDLEITRV"
     gene            1280..1687
                     /locus_tag="PK125_09445"
     CDS             1280..1687
                     /locus_tag="PK125_09445"
                     /inference="COORDINATES: protein motif:HMM:NF020940.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NIL domain-containing protein"
                     /protein_id="HPB38365.1"
                     /translation="MKKRIILRFKKNTIDKPIVYKLVKDYDLVFNILRASILPRAESM
                     MVMEIEGTEDNFNRGIAYLRGLNIETAPMEQDINRDEEKCVHCGMCTSVCAPDALYLD
                     RETMKVHFDYKKCVACELCVRVCPVKAMNVFFD"
     gene            1695..2858
                     /gene="nifS"
                     /locus_tag="PK125_09450"
     CDS             1695..2858
                     /gene="nifS"
                     /locus_tag="PK125_09450"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR03402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase NifS"
                     /protein_id="HPB38366.1"
                     /translation="MKKIYLDHNATTPVLPEVVEFVKPFFSDFFGNPSSIHWAGRDVR
                     VHYDNAREHVASMVNAKPEEIVFTGCGSESDNHAIKGVAFSRKSRGNHIITTAIEHPA
                     VLNTCRYLETVGYRVTYLPVDQDGQIEPDDVRKAIEKETILISVMYANNETGNLLPIR
                     EIGAIAREHDVIFHSDMVQALGKIAIDVDSLNVDLASFAGHKVYAPKGVGVLYIRQGL
                     DIENLIHGGHQEGGRRAGTENVIGAIAFGKANEIISHHMDEENKRIDGLRQKLLEGIL
                     ERIDHVKFNGHRTHRLPNTLNLSFEFVEAESLLIALDLKGIAVSSGSACSSGSGEPSH
                     VLRAMDIPVETCLGTIRLSLGRSNTEEDLDYTLSVLPEIVARLRAMSPLYRKK"
     gene            2959..4230
                     /gene="mtaB"
                     /locus_tag="PK125_09455"
     CDS             2959..4230
                     /gene="mtaB"
                     /locus_tag="PK125_09455"
                     /inference="COORDINATES: protein motif:HMM:TIGR00089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N(6)-L-threonylcarbamoyladenosine(37)-C(2))-
                     methylthiotransferase MtaB"
                     /protein_id="HPB38367.1"
                     /translation="MKFFIHTTGCKANQWDSYIITNSLKGTGHSPGTISNADFIVINA
                     CTLTEGAERDLRRFINRSRGINSGARIILAGCHAQVYPEQAYGADLVLGQEEKFRISE
                     FLCLNGLFVSKTRSIPLESIIADGLPWQRTRVFLKIQDGCDRFCSYCVVPLARGKPRS
                     RNSVEIFDAMKTFKERGVKEVVLTGIEISEYRDPLTGKDLKGLLEDLEQKETPPRIRI
                     SSIDPLYLDDECIQIIRSSKKIMRSLHIPLQSGSDAILKRMGRQYTTEYIRNLVRKLN
                     TMIHGVGIGMDVIAGFPTEEESHFLETSHFLSKLDIYYLHVFPFSPRKGTKAATMENA
                     VNGTVKKRRVSLLRKLDSEMRIKFYKRFVGQKAWILAEGKMHQNLYMRGYTENYIPVC
                     IPYEKKLENNIVNVTIKGVQDRLVIGEPNVV"
     gene            4339..4638
                     /locus_tag="PK125_09460"
     CDS             4339..4638
                     /locus_tag="PK125_09460"
                     /inference="COORDINATES: protein motif:HMM:NF014389.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HPB38368.1"
                     /translation="MFVFGNFVTGIAYILDMLLTVYFWIILIRAVLSWIQPNPYNPAV
                     RIIYNLVDPVTYRISRKIPTRIGAIDFAPFILMIIIVFLQRFLIRSLFDLGTRMN"
     gene            4740..4991
                     /locus_tag="PK125_09465"
     CDS             4740..4991
                     /locus_tag="PK125_09465"
                     /inference="COORDINATES: protein motif:HMM:TIGR00251.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF167 domain-containing protein"
                     /protein_id="HPB38369.1"
                     /translation="MIIEVRVITNAKKKELKFEGDSLKVKITALPKEGRANTALIEYL
                     ADFFGVKRSEVKIIKGEKEKRKLISLPLEEEQIAKAMGR"
     gene            5104..5622
                     /locus_tag="PK125_09470"
     CDS             5104..5622
                     /locus_tag="PK125_09470"
                     /inference="COORDINATES: protein motif:HMM:NF014657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceD family protein"
                     /protein_id="HPB38370.1"
                     /translation="MIVKLSDIEDRLIVKGEIASPVFENLEEEQFTIESPVRFELVIT
                     KLDDNVRISGPVRSTVIMSCSRCLEEFSCPLETYLDIEVEPKRLLSHEAEVELRPDDL
                     DIYYYDGDEINLDPLIYDEVLLNLPIRPLCREDCAGLCHICGKNKNVESCACSQYAST
                     VLGEKLKNFLNT"
     gene            5633..5815
                     /gene="rpmF"
                     /locus_tag="PK125_09475"
     CDS             5633..5815
                     /gene="rpmF"
                     /locus_tag="PK125_09475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000290472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HPB38371.1"
                     /translation="MAVPKRKTSKSSRDKRRSHDRVIAPNVTLCSNCKEPKLSHIVCP
                     KCGYYKGKQYLKIGEA"
     gene            5821..6816
                     /gene="plsX"
                     /locus_tag="PK125_09480"
     CDS             5821..6816
                     /gene="plsX"
                     /locus_tag="PK125_09480"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: protein motif:HMM:TIGR00182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HPB38372.1"
                     /translation="MVRIAVDGMGGDFAPQAIVKGAEQALKEKVAEIILVGDKARLVP
                     FLENPDEIEIVHTDEYVEMTASPSAALRKKRGSSVNKSFELVRTGKAQGVVTAGNSGA
                     AMAFAIFTLGRIGLVERPAIATLHPNVSKGTITVVLDGGGNVDCRPVHLVQFAIMGSA
                     FAKSAFGIEMPRVGLLSNAEEETKGNDLTREAHAMIKELGLNYLGYVEGNHVHNGRVD
                     VVVCDGFVGNIALKITEGVAESLMAFLRERIEKSLKNKMGYLLMKEIFDELAKKTDYS
                     EYGGAPLLGVDGVCIICHGRSNERAIKSAISMAAGFVRKNLNESIKETMIDHFRG"
     gene            6830..7990
                     /locus_tag="PK125_09485"
     CDS             6830..7990
                     /locus_tag="PK125_09485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016293395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HPB38373.1"
                     /translation="MDYFLTDDQKHIQSLARRIAEEKVVPIRAELDEKEEFPWSVMKV
                     CADTGLFGVSIPPEFGGMGGGCFENCIVVEELSKACLGVSVSYAASLLGAYPILIGGS
                     DEQKKKYLKEIASGKKLAAFALTEANAGSDAQGIRTEAKKVGDEYILNGTKQWITNGG
                     EAEIYTVVAMTDRSRGGRGATAFIVENGTEGFSFGKKEKKLGIRASATRELVFQDCKV
                     PKENVIGREGMGFIFAMRTFDKTRPGIGAQAVGVAAGALEAAIHYAREREQFDKKIAS
                     FQAIQHMIADMATQVEAARALVYAVARYIDSNPRDSSKVSAMSKVFPSDVAMKVVVDA
                     VQIFGGYGYMKEYPVEKMMRDAKILQIYEGTNQIQRNVIGLELIKEAASKKK"
     gene            8013..8948
                     /gene="fabD"
                     /locus_tag="PK125_09490"
     CDS             8013..8948
                     /gene="fabD"
                     /locus_tag="PK125_09490"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: protein motif:HMM:TIGR00128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HPB38374.1"
                     /translation="MKRIGVVFPGQGSQYVGMGKKLYDRFEEVKDFFAGADKALGFPI
                     SELIFNGPEEELRQTFNTQPALVLVSYAVWRIFQKETKTTPHLVSGHSLGEYTALLAS
                     GYFTFEDALKITRKRGLLMEESCPKGKGGMVALIGANMEKIKPVLNEISHDEYVAVPA
                     NMNSSEQVVLSGDMGALKEAVDRLKGVGYKKAVFLNVSGPFHSPLMKEAAEKLKTELQ
                     GLNKDDLTIPVVSNVDATPEKSKDLIIDKLYRQMFSPVLWETCVKRMAREGVECFIEV
                     GPQKVLSNLIKRIVPDVPCYNVEEYEDIEAIKDVL"
     gene            9019..9744
                     /gene="fabG"
                     /locus_tag="PK125_09495"
     CDS             9019..9744
                     /gene="fabG"
                     /locus_tag="PK125_09495"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010966837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HPB38375.1"
                     /translation="MKDTVTIVTGGSQGIGRSIAEFLAAKGGDVVIFDIADSSDIVQY
                     IRQKGQRSEFYQIDVSNFNMVGEAVDKVIKDMGRVSNLVNNAGITADKLLLRMKEEDW
                     DRVLQINLKSAFNCTKAVVRYMLKTGGSIVNISSIAGVMGNAGQANYAASKAGLIGFT
                     KSVAREYGERGIRVNAVAPGFIKTRMTESLDEKSRETMFKAIPLGRLGEPDDIAHVVY
                     FLLSEYGSYITGEVINVNGGLYM"
     gene            9847..10083
                     /gene="acpP"
                     /locus_tag="PK125_09500"
     CDS             9847..10083
                     /gene="acpP"
                     /locus_tag="PK125_09500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005779510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HPB38376.1"
                     /translation="MTVAEKVKEMIIKQLDSKESEVVPEARFIDDLGADSLDIVELIM
                     ALEDEYGLEIPDEDAEKIETVGDAIRYIEEHIKK"
     gene            10099..11340
                     /gene="fabF"
                     /locus_tag="PK125_09505"
     CDS             10099..11340
                     /gene="fabF"
                     /locus_tag="PK125_09505"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: protein motif:HMM:NF004970.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HPB38377.1"
                     /translation="MKRRVVVTGIGLVTPCGIGTDNVWNNILSGKSGIGPITRFNTDR
                     FDTKFAGEVKDFNPEDYVQPKEVKKMDLFIHYALAAAHIAVKDAGLDMGKEDPERVGV
                     VVGTGLGGLPTIEKYHSVLLERGPGRITPFFIPMLIANEAPGHIAIQHGIKGPNLCIV
                     TACATGAHSIGDACRIIQYGDADVMVAGGSEANLTPLTVGGFNAMKALSTRNDDPMKA
                     SRPFDRDRDGFVVAEGSGIIILEELEHAKKRGARIYAEMAGYGYNGDAYHITAPCPDG
                     DGFIRCMRMALRDAHISPDHVDYINAHGTSTKLNDYIETLAIKEVFKEKAYRIPVSST
                     KSMTGHLLGAAGAVEAVFSILSIRDQVCPPTINYENPDPECDLDYVPNTARKCTINTV
                     VSNGFGFGGTNSTLVFRRFIE"
     gene            11337..11786
                     /gene="rpiB"
                     /locus_tag="PK125_09510"
     CDS             11337..11786
                     /gene="rpiB"
                     /locus_tag="PK125_09510"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HPB38378.1"
                     /translation="MKVVVGSDHAGYELKEQVKTILTEKGHTVIDVGTGSPASVDYPD
                     FGIEAATLVGRGEADRGVLVCGTGIGMSIVANKVKGVRAALIDDLYSAIQSRKHLDAN
                     ILVLGGRVTGRDLAAEITRVWLDTPFEGGRHSKRLEKIEKYEKDNLK"
BASE COUNT         3574 a         2318 c         3082 g         2987 t
ORIGIN      
        1 cctgaggaaa agcgacacga ttgcaaggat gggaggagac gagtttcttg ttatagttcc
       61 tgaaacggaa aggccggagg atgtggccac agttgctcaa aagattctgg gggtatttca
      121 aatacccttc gactgtaata gcttcaaaca atcgagttct acgagtatag gggttgcaat
      181 gtatcctaaa gatggagaca acagcgaagt attaatacga tgtgctgaca tagccatgta
      241 tagtgcgaaa gcaaaagggg gcaacagctt ctgtatctat accccggaaa ttgaaagcaa
      301 acagtaaatc ggttgcagtc ctttattctt ttgtagtccc tgaacagaca cggaaaaaca
      361 ggtcagtctg ataatcataa ggtatcatga acatgaagga aatgggtttg acaccattgt
      421 aactgactgg tatatttagt tggttttatg ttttttactc caccactgat catgggctcc
      481 gcattgcgat cggccacagc gagtgaattg tgtacgaaga aagattttac agaaacatag
      541 caagacctgc agatttactc tgctatgggg tcacctacaa ggagacagat ctctggtgct
      601 gtacaaaaac tgatttgaga aaggtcatag aggatcgtgt tttttattac cggcatcaac
      661 ttgaagaata tatcaggaca aatcctattt tcaacgagag ccttttgccc atcaaccatg
      721 accaatttgc tcccgtcatc gtcaaagaga tgatttctgc atcagcacgg gttggggtgg
      781 gacccatggc tactgtagcc ggggcgattg ctgaatttct gggaagggat atagcgcacc
      841 tcacagacga gtatatcatt gaaaatggcg gtgatatcag tatcaaaaca ttacgggaga
      901 gaatgattct tgtatacgca aaaaaatctc cttttagcca gaatattgcg atcagaatta
      961 aggagaggga caagccctat ggagtatgta catcctctgc aactgtaggc cactccctga
     1021 gccttggaaa agctgatgca gtatgtgtta tcgctgactc agcacttttt gcggatggac
     1081 ttgcaactta tattggtaat agtgtaaaaa gtaaagatga tataggtggt gcaatagagg
     1141 caggaaaaac attctccaat gttatgggtc tattgataat tgtaggcgat agtttaggcg
     1201 tctggggaga cctggagatc acaagagtct gaccatcggt ttgcaagcct taagccctaa
     1261 aacaaagcac gaggtctata tgaagaaaag aatcattctg aggtttaaaa agaacactat
     1321 cgataaaccc atagtctata aacttgtgaa agattatgac ctggttttca atatcctgag
     1381 ggcgagtatt ttgccgagag ccgaatcaat gatggtcatg gagattgagg gtacagagga
     1441 caacttcaac agggggattg catacctgag gggcctgaat attgaaacag cccctatgga
     1501 gcaggatata aacagggatg aggagaagtg tgtccattgc gggatgtgta cgagtgtctg
     1561 cgctccggac gctctttatt tggacagaga gacaatgaag gttcattttg actacaaaaa
     1621 gtgtgttgcc tgcgagcttt gcgttagagt ctgtcctgtt aaggcaatga acgttttctt
     1681 tgattaacaa tcctgtgaaa aaaatatacc ttgaccataa tgcaacaacg cctgttctcc
     1741 ctgaagtggt tgagttcgtt aaaccattct tttcagattt tttcgggaat ccttcaagca
     1801 ttcactgggc cggcagagat gttagggttc actacgataa tgcccgcgaa catgttgcaa
     1861 gtatggtcaa tgcgaaacca gaggagatag tctttactgg ctgcgggtct gaaagtgaca
     1921 accatgccat taagggagtg gcattctcca gaaagagcag gggaaaccac attattacaa
     1981 cagccattga gcatcctgct gtgctgaaca cgtgcagata cctcgaaaca gtggggtata
     2041 gagtaaccta ccttccggtt gaccaggacg gtcagataga acccgatgat gtgaggaagg
     2101 ccatagaaaa ggagacaatc ctgatctctg ttatgtatgc gaataacgaa acaggaaatt
     2161 tgcttcccat ccgtgagata ggcgccattg cgcgagagca tgatgttatt tttcattctg
     2221 atatggtgca ggccctgggg aagattgcta tcgatgtaga ttcattgaat gttgaccttg
     2281 caagcttcgc aggtcataag gtttatgctc caaagggggt tggggttctc tacatcaggc
     2341 aggggctgga catagaaaac ctcattcacg gcggacatca ggaaggaggg aggagggctg
     2401 gcacagaaaa tgtcattggt gctatagcat ttggaaaagc taacgaaatt atctctcatc
     2461 atatggacga ggaaaataag agaatagacg gattaagaca gaaactcctt gaaggcattc
     2521 tggaaagaat agatcatgtg aagttcaacg gacaccgaac acacaggctg cccaacaccc
     2581 tcaatctgag tttcgaattt gtggaggcag agtctcttct tatcgcactg gacctgaaag
     2641 gtattgcagt atcctcggga tcagcatgct catcaggctc aggcgagccc tctcacgtat
     2701 tacgggcaat ggatatacct gttgagactt gtctgggcac tatccgactg agcctgggca
     2761 gaagcaacac tgaagaagat ctggattata ctttgtctgt tctgcctgag atcgttgcgc
     2821 gtttgagggc aatgtcaccg ctgtatcgga agaaatgacc agttctcagc tcgaagctga
     2881 tagctgacag gcaaagccat acagcctttc agttatttcc tgtgagctgt atacaagcta
     2941 caacctatga gctccaaaat gaaattcttc atccacacaa ccggttgcaa agcaaatcag
     3001 tgggattctt acatcataac aaacagcctg aaaggcacgg gtcattctcc cggcaccatt
     3061 tcaaatgcag attttattgt tataaatgcc tgtacactca ctgaaggggc tgagagggat
     3121 ttgcggcgtt tcatcaatcg gtcgcggggt atcaactcag gagcacgaat aatccttgca
     3181 gggtgccatg cacaggtcta tcctgagcag gcctacggcg cagaccttgt tctcggccag
     3241 gaggaaaagt ttcgaatcag cgagttcttg tgtttaaatg gtttgtttgt cagtaagaca
     3301 cgctcgattc ctttggaaag cataattgct gatggtttgc cctggcaaag gacaagggtt
     3361 tttctgaaga tacaggatgg ctgcgaccgg ttttgcagtt attgtgttgt tcctctcgca
     3421 cggggcaaac cgaggagcag gaattcagtt gaaatttttg atgccatgaa aaccttcaag
     3481 gaaagagggg ttaaggaggt agtgctcacc ggcatagaaa tctcagagta cagagaccct
     3541 ttaaccggaa aggacctcaa gggtttgctg gaggaccttg aacagaagga gacaccaccc
     3601 aggatccgga taagctccat cgatcctctc taccttgatg acgaatgtat tcaaattatt
     3661 cgctcttcga agaaaatcat gagaagcctt cacatacccc tccagagcgg gtccgatgct
     3721 attctaaaaa ggatgggcag acagtacacc acagaataca taagaaatct tgtaagaaaa
     3781 ctgaatacca tgattcacgg cgtggggatt ggcatggacg ttatcgcagg gtttcccaca
     3841 gaagaagaga gccacttcct ggaaaccagt cattttctca gtaaactcga tatctactat
     3901 ctccatgttt tccctttctc acccaggaag ggaacaaagg cagcaacaat ggaaaatgcg
     3961 gtgaacggta cagtgaaaaa gcgacgggtc agcctgctgc gtaaacttga cagtgaaatg
     4021 cggataaagt tctataagag gtttgtggga caaaaggctt ggatactcgc tgagggaaag
     4081 atgcaccaaa acctttacat gagaggctat acggaaaact acatccctgt ctgcattccc
     4141 tacgaaaaaa aacttgaaaa taatattgtt aacgttacaa taaagggggt acaggataga
     4201 ctcgtcattg gagaaccaaa tgtggtctga tggacttctt tattataagt aagtcaggaa
     4261 gtatttcgta tatcgtatgt tgtatcctgc agaggaactt tacgatatgc aaccaattat
     4321 tcacaatcag ggggtaacat gtttgttttc ggaaatttcg ttacagggat agcatatatt
     4381 ttggacatgt tacttactgt ctacttctgg ataatcctga tacgggccgt cctgtcctgg
     4441 atccaaccca acccttataa cccggcagta cggattatct ataatcttgt tgaccctgtt
     4501 acctacagaa tatcgagaaa aatccctaca aggattggtg cgattgactt tgctcctttt
     4561 atactcatga tcatcatcgt ttttctccaa agatttctga taagaagtct gtttgacctt
     4621 gggacgagaa tgaattagag ctcacctccc agcgaagcga ggtaaatctg caaaggcaat
     4681 gatggttacc gtcaagcaaa cacgaacatc tcttcccaca ggtccatgga cacatccata
     4741 tgatcattga ggttcgtgtc attaccaatg caaaaaagaa ggagctgaaa tttgaaggag
     4801 attctttaaa agtaaaaatt accgctttgc caaaagaagg cagggcaaac acggctttga
     4861 tagagtatct cgctgatttt tttggcgtga agaggtcaga ggttaaaata attaaaggtg
     4921 agaaggaaaa aagaaagctc atctccctcc cccttgaaga agaacaaatc gcaaaggcta
     4981 tgggcaggta aatggccggc taatagcctg attatagaaa actgcaagag ttacaaacag
     5041 ctaatttact tgacattcaa cggctaaacc aattatatta tcgggtttta gaggtaggca
     5101 gcattgattg tcaaactttc tgacatagag gacagactca tcgtaaaagg tgagattgca
     5161 agcccggtat ttgaaaacct tgaggaggaa cagtttacga tcgagtcgcc tgtccgcttt
     5221 gagttggtga taacaaaact tgatgataac gtgagaatct ctggcccggt cagaagcact
     5281 gtgataatgt cctgctcccg ttgcctggaa gagttttcat gtcctttaga aacatatctt
     5341 gatatagagg tggaaccaaa aagacttttg tctcatgagg ctgaagtcga gttgaggccg
     5401 gatgatttgg atatttacta ttatgatggt gacgagatta accttgatcc tttgatttac
     5461 gatgaagtgt tgctcaatct cccgatcaga cctttgtgca gggaagactg cgcaggactt
     5521 tgccatatat gcggaaaaaa caaaaatgtc gaatcctgtg catgcagtca atatgcaagt
     5581 acagttttag gggagaaatt aaagaatttt ttgaatactt aaggagaaca aaatggcagt
     5641 tccaaaacgc aaaacctcaa aatcatcgag agacaaaagg cggtctcatg acagggtcat
     5701 agccccaaat gtaacgctat gttctaactg taaagagcct aagctttctc atattgtgtg
     5761 tccgaaatgc ggatactata agggtaaaca gtaccttaaa atcggggaag cctgatctaa
     5821 atggtcagaa tagcagttga tggtatgggg ggcgattttg cgcctcaggc tatcgtgaag
     5881 ggtgcagaac aggctttaaa ggagaaagtt gctgaaatta tccttgttgg cgacaaggcg
     5941 aggcttgttc catttttaga gaaccctgat gaaatagaga ttgttcatac tgacgaatat
     6001 gtggagatga cagcatctcc ttccgctgct ctgcgaaaga aaagagggtc atcagtaaac
     6061 aagtcttttg aactggtgag aactggcaag gctcagggtg ttgtaacagc aggcaactca
     6121 ggggctgcta tggcctttgc catattcacc cttggaagaa ttggtctggt ggaaaggccg
     6181 gcaattgcca ccctgcatcc gaatgtttca aagggcacaa taactgtagt ccttgatgga
     6241 gggggaaatg ttgactgcag acctgttcac ctggtccaat tcgcaataat gggaagtgcc
     6301 tttgcaaaaa gcgcctttgg catcgaaatg ccccgggtcg gacttctgag caatgcagag
     6361 gaagagacca aagggaatga tctgacaaga gaagcgcatg caatgattaa ggagctcggc
     6421 cttaattatc ttggttacgt tgaaggaaat catgttcaca acggtcgtgt ggatgttgtg
     6481 gtatgtgacg gttttgttgg caacatagcg ttaaagataa cagaaggcgt ggcagagtca
     6541 ttgatggcct tcctgaggga gcgcatagaa aaaagcctga aaaacaaaat gggttacctt
     6601 ctcatgaagg aaatttttga tgagcttgca aaaaaaacag attattcgga atatggagga
     6661 gctcccctcc tcggtgtaga cggtgtctgt attatctgcc acggcagatc caatgagcgg
     6721 gctataaaaa gtgcaatttc catggccgca ggctttgtaa gaaaaaactt aaacgagtcg
     6781 ataaaggaga cgatgatcga ccatttcagg ggttaaagga gagttgtcca tggattattt
     6841 ccttacagat gatcagaagc atatccaatc gttagcacga agaattgccg aagaaaaggt
     6901 tgtgcctata cgggctgagc tggacgaaaa agaagagttt ccctggtctg tcatgaaagt
     6961 ttgtgcagat acggggttat ttggggtgag tataccccct gaatttgggg gcatgggcgg
     7021 aggatgtttt gaaaactgta ttgtggtgga ggaactgagc aaggcctgcc ttggggtttc
     7081 ggtcagctat gcagcgagtc ttcttggggc atatccaatc ctgatcggtg gctctgatga
     7141 gcaaaaaaag aaatatctca aagagatagc aagtggaaaa aaactggcag cttttgccct
     7201 aacagaggca aacgctggaa gtgatgctca gggcataaga accgaggcaa aaaaggtggg
     7261 cgatgaatat atcctgaacg gcacaaagca gtggatcacc aacggaggcg aagctgagat
     7321 ttatactgta gtagccatga ccgacagaag caggggtggt aggggagcaa cagcatttat
     7381 cgtggagaat ggcacagaag gcttttcttt tgggaaaaaa gagaagaagc ttggcatcag
     7441 agcttctgcc acaagagagc ttgtatttca ggattgcaaa gttccgaagg aaaatgttat
     7501 aggtagagaa gggatggggt tcatctttgc catgagaact ttcgacaaga cacggcctgg
     7561 cattggcgcc caggcagtgg gagtagctgc tggagcactt gaggctgcca tacattatgc
     7621 cagggagaga gaacagtttg ataaaaagat tgcttctttt caggctatcc agcatatgat
     7681 cgcagatatg gcaacccagg ttgaagcagc acgtgccctt gtctatgcag ttgcgagata
     7741 tattgacagc aacccgagag actcatcaaa ggtctccgct atgagcaaag ttttcccgag
     7801 tgatgttgca atgaaggtag ttgtggacgc agttcagatt tttggtggtt acgggtatat
     7861 gaaagaatac ccggtggaaa agatgatgcg agacgcaaaa atactccaga tttatgaagg
     7921 gacaaaccag atccaaagaa acgtcattgg cctcgaattg atcaaagaag cagcctcaaa
     7981 gaaaaaataa agggagataa gtcgacaatt ctatgaagag aataggtgtt gtctttccag
     8041 gtcagggatc gcagtacgtt ggcatgggga aaaaactgta cgatcgcttc gaagaggtga
     8101 aggatttctt tgctggagct gacaaagccc ttggttttcc tatctcggag cttattttta
     8161 acggtcctga ggaggagctg aggcagacct ttaataccca gcctgccctt gtccttgtga
     8221 gttatgctgt ctggcggatc tttcagaagg agacgaaaac aacccctcac ctcgtttccg
     8281 ggcacagcct tggggagtat acagcccttc ttgcaagcgg atatttcact tttgaagatg
     8341 cactcaagat taccaggaag agaggattgc tcatggagga atcctgccca aaaggtaagg
     8401 gtggtatggt tgcccttata ggcgccaaca tggaaaaaat caaaccagta ctcaacgaaa
     8461 tatcacatga tgagtatgtt gctgttcctg caaacatgaa ctcttctgaa caggtagtct
     8521 tgtccggaga tatgggcgcc ctcaaagagg ctgtggacag attaaaaggg gtaggatata
     8581 agaaggcggt tttcctgaat gtttccggtc ctttccatag tcctctcatg aaggaagcag
     8641 cagaaaaatt gaaaacagaa cttcagggcc ttaacaaaga cgatttgact atacctgttg
     8701 tatcaaacgt tgatgcaacc cctgagaaaa gtaaggatct tattatagat aagctctatc
     8761 gccagatgtt ctcacctgtt ctctgggaga catgcgtgaa gaggatggcc agggaaggcg
     8821 ttgagtgttt catagaagta gggccccaga aagtcctcag caaccttatc aagagaatag
     8881 tgccggatgt cccctgctac aacgttgaag aatatgaaga catagaagcc attaaggatg
     8941 ttttatgaaa ctgtatgaaa tattagaggg ttccctattg cctaaaacct atagcccctc
     9001 gccaaaagag gtttattcat gaaagatact gtaactattg tcaccggtgg ttcacagggc
     9061 attggaagaa gcatagcaga atttcttgct gctaaagggg gcgatgtagt aatttttgat
     9121 attgctgaca gcagcgacat tgtacaatat atcagacaaa aaggtcagag gtcggaattt
     9181 taccagatcg atgtatccaa cttcaacatg gtcggtgaag ctgttgataa ggtaatcaag
     9241 gacatgggcc gtgtcagcaa cctggtaaat aatgccggta ttactgcgga taaacttctt
     9301 ctgagaatga aggaagagga ttgggataga gtactacaaa taaatctgaa aagcgctttt
     9361 aattgcacca aggctgttgt tcgttacatg ctgaagactg gcggatcgat tgtcaacatc
     9421 agttctattg caggggtaat gggcaatgct ggtcaggcaa attatgcggc aagcaaagcc
     9481 ggtctcatag gttttacaaa gagcgttgcc cgagaatacg gggagcgggg cattagggtt
     9541 aatgctgtgg cccccgggtt tataaagacc aggatgacag aatctcttga tgaaaaatca
     9601 agagagacga tgttcaaggc tattcccttg ggaagattag gggagcctga tgatattgct
     9661 catgttgtgt atttcctgct ttctgagtac ggaagttaca ttacagggga ggtgataaac
     9721 gtcaacggtg gtttgtacat gtaaagttca ctattaggtg atcggtgtta ttgcagcgaa
     9781 gaacatgacg aaatgtctcc actgcttcct gaaaacctgt gtttttaaaa aataggaggt
     9841 gattaaatga cagttgcaga aaaagtaaag gaaatgatca taaagcagct tgactcaaaa
     9901 gagtcagagg ttgtgccgga ggcgaggttt atcgatgacc tcggagcgga ttcccttgac
     9961 atcgttgagc ttattatggc tttggaagat gagtatggcc tggaaattcc tgacgaagac
    10021 gcggaaaaga tagagacggt cggagatgca atacggtata ttgaagagca cataaaaaaa
    10081 taaaaggagt taacagtctt gaaacgtagg gtggtagtga cagggatagg actcgttacg
    10141 ccctgtggta taggaaccga caatgtttgg aataacatcc taagcggaaa gtctggtatc
    10201 ggtcctatca cacggtttaa tacggaccgt tttgatacaa aatttgccgg agaggtgaag
    10261 gattttaatc cggaggacta cgttcagccc aaagaagtta aaaagatgga tttatttatc
    10321 cattatgccc ttgccgccgc acacatcgca gtaaaagatg cgggacttga tatgggaaag
    10381 gaagatcctg agcgtgtcgg ggttgtggtg ggcacaggtc ttggagggct tccgactata
    10441 gagaaatatc acagcgtgct cctggagagg ggcccgggca ggataacacc cttttttatc
    10501 cccatgctga tagcaaacga ggcgcctggc catatagcaa tacaacacgg aataaagggc
    10561 cccaaccttt gtatcgtcac agcctgtgca acaggagcac actctattgg cgacgcctgc
    10621 aggattattc aatacggcga cgcagatgta atggtggctg gaggaagtga agcaaacctg
    10681 acccccctta cggtaggggg gtttaatgca atgaaagctt tgtctaccag gaacgatgat
    10741 cctatgaagg catcgaggcc atttgatcga gacagagatg gctttgtagt tgccgaaggt
    10801 tcagggataa tcatcctgga agagcttgaa catgcgaaaa aacgaggcgc tcgaatttat
    10861 gcggagatgg caggctacgg atataacggg gatgcctacc atatcacagc gccatgcccg
    10921 gatggtgatg gcttcatccg gtgcatgaga atggccctca gggacgcaca tatttcacct
    10981 gaccatgtag actatataaa tgcccacgga acatcaacaa aattgaatga ttatattgaa
    11041 acccttgcga tcaaggaagt atttaaggaa aaagcataca ggatacctgt gagctcaaca
    11101 aaatccatga caggccatct cctgggggct gcaggtgctg tggaggctgt cttttcgatc
    11161 ctcagcatca gggatcaggt atgcccgccc acgatcaact atgaaaaccc tgatccagaa
    11221 tgtgaccttg attacgtgcc aaatacagca agaaagtgta ccattaacac ggtagtatcg
    11281 aatggctttg gttttggagg aacgaactca accctggtgt tccggagatt catagaatga
    11341 aggttgtagt aggatctgat catgccggtt atgaactgaa agaacaggta aaaacaatcc
    11401 ttacagaaaa ggggcatact gttatcgatg ttggtaccgg atcacctgcg tcagtggatt
    11461 accctgattt cggaatcgaa gcggccacac ttgttggccg tggtgaggct gaccgcggag
    11521 tgctcgtctg tgggactgga attggcatga gcatagttgc aaacaaagtg aagggtgttc
    11581 gggcagccct tattgatgat ctctacagtg ctattcagag cagaaagcac ctcgacgcaa
    11641 acatccttgt gctcgggggg agagtaaccg gcagagatct tgctgctgag attacacggg
    11701 tatggctcga tacacctttt gagggcggca gacacagtaa aaggcttgag aaaatagaga
    11761 agtacgaaaa ggacaattta aagtaggtat gaaaatggac ggcaaggaca gacctgtttt
    11821 cgatcttttg aaacgcgagc tggaaaggga agagtacagc cttattctga ttgcctctga
    11881 gaattatgtg gatgaagaaa tccttgaagt ccaggggtgc gttttaacaa acaaatatgc
    11941 agaaggatat ccttcaaaac g
//
LOCUS       DAOOMC010000087        11731 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdus sp. isolate AMR_MDS_2685
            NODE_6102_length_11731_cov_5.699897, whole genome shotgun sequence.
ACCESSION   DAOOMC010000087 DAOOMC010000000
VERSION     DAOOMC010000087.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864760
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdus sp. (wastewater metagenome)
  ORGANISM  Syntrophorhabdus sp.
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae; Syntrophorhabdus.
REFERENCE   1  (bases 1 to 11731)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11731)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,729
            Genes (coding)                    :: 2,708
            CDSs (with protein)               :: 2,708
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 7 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11731
                     /organism="Syntrophorhabdus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6102_length_11731_cov_5.699897"
                     /isolate="AMR_MDS_2685"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2699756"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(201..1505)
                     /locus_tag="PK125_09515"
     CDS             complement(201..1505)
                     /locus_tag="PK125_09515"
                     /inference="COORDINATES: protein motif:HMM:NF037981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="solute carrier family 23 protein"
                     /protein_id="HPB38379.1"
                     /translation="MKPIDLKHQLDEWPSFGELLLLGLQWLAISIPGIIIVGKVVGII
                     HDSAVVGQITYLQRMCFMVSIALFAQILWGHRLPLILGPSTILLIGIISGRNYNADVI
                     YTSIMIGGAALTALSLSGLFGYIQRLFTPRVVAVVLILIAFTLLPMILNLVTKPHRAV
                     LAEANLIFSIILTGCMFLSYRLVGPLWRSTMILWAMIAGSTVYYLIFPFAINLEAIID
                     QRFAGFFLTGPITRWSLESGVLVSFLFCFFALSINDLGSIQSMQGILNPTGMSSRINR
                     GITFTGVANIIAGFLGVIGPVNFSLSPGVIRATKSASRFALIPASILLCLISFSPLVI
                     NLLDNIPPAVIGSSLIYILSFQVSAGKDIAFHSKKDFSVQNILTIGIPTLLGTIIAFL
                     PPSSFRLLPIFLKPIIGNGFVVGVLTVLILEHLILKKNRTRM"
     gene            complement(1514..3061)
                     /locus_tag="PK125_09520"
     CDS             complement(1514..3061)
                     /locus_tag="PK125_09520"
                     /inference="COORDINATES: protein motif:HMM:NF005760.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
                     /protein_id="HPB38380.1"
                     /translation="MNGAEVILQTAAAAGIEVCFANAGTTELPLVAAFDTVSGIHPVM
                     ALFEGVCSGAADGYGRVKERPGLTLLHLGPGFANGIANLHNARRARTPLINIIGQHTT
                     WHIDADAPLTMDVESLARAVSDWTKTTTSANELSSAVTEAYLESLPGKIATLIVPNDH
                     LWAEAGNTICAPAPLKFDSPDMTLIKAAADLLRTERRTAVMLGGKALRGKGLSTAGRI
                     QSNTGCDLITETFPGYVERGKGLPDVMRIPYFPEPAQALLSQYDTIILADAKEPVTFF
                     GYFGVDSHLLTKDQKKLSICSGTQNIVEVLECLEDELNTASASNRGWNLPQGIKPLLP
                     RGTLTAEKACQVLAALQPEGAIIVDEGITSGIPYYPLTAFTPPHVVLTIVGGSIGYGM
                     PCAVGAAVASPDRPVINFQADGSAMYTLQALWTEAREGLNVKTLICSNRGYNIVRVEL
                     QRAGIADPGPKALSLADIDHPKLDWVSISSGLGVPAVSVSTAEDLAKELTKTLSDPGP
                     FLIEMIL"
     gene            complement(3923..7156)
                     /locus_tag="PK125_09525"
     CDS             complement(3923..7156)
                     /locus_tag="PK125_09525"
                     /inference="COORDINATES: protein motif:HMM:NF016292.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate binding protein"
                     /protein_id="HPB38381.1"
                     /translation="MRRISKVFVKQGYFQYEDKVKRREKSVRTGLYLSLSVACFLVFF
                     AVSNAVPIPQPPDQNRHVLIINSYHKGYPWTDNVLAGIESVLKDGKSNITLHIEFMDT
                     KRVVDKNYFYHLFLYYRSKFEKVRFDLIIVSNKDALNFAMRYRDLLFPKIPVVFCGVD
                     AQNEMMRQKAPLVTGVLEDFDIKATLDIALKLHPNTREIIAINDGTAIGAVKKQIFKD
                     ALAATGKNLIATIIDDPVLEDFEKLLKDKDKDFVVILMGSFRESSGAAVPIEVSTPIL
                     TRYNIPLYALVEYYLGYGIIGGKLISGFHQGMLAAKMGLKILQGEPVDRIPVEHKSQN
                     IFMFDYNQLMKFGINQDTLPEGSIIINEPKPGPSEDKKIKWEVIAVPFSLAIIATFLV
                     LYLLSRKRREELLGKLSRDHEKKIEARTEELMKSNTDLKLKITEMKQAEAGLVEERLK
                     HLTLFQRAPSPFFVVNRDYRYSDFNEKMVELFECTLEEFRKMDASNLIPKKLLARLFE
                     SQGPVNNMESFETQLEVRGKTKRFLFSIVPVVLSGERFVYGVGQDMTQLIQSQDISKQ
                     QEKLLENLFEVLSDTAIISDRKGTIKYVSEGFLKVFGFSRDDFQSIDAWLQKVCSDES
                     LRDVAMNAWLRAVKTRNIGEEILQPIEGKLATKDGASLERELWFIPMDDMVVSLIRDI
                     TRKKVYGERAPVSRNQESVETLAAGIAHDFNNLLLVILGNISLAKTSLTREDKAFEHL
                     IDAEKASMMTKDLIQQLITFSKGGELSKRAMVITSLVMEITRSTLSNSNIRGKYTMSD
                     DLFPVEVDEGQIRQVVHIILRNAMEAMPQGGTVAISFENVKVSREDYLPLKQGDYVKI
                     SFQDQGSGIKKEHLERIFDPYFTTKDAGSQKGVGLGLAIAYSIVKKHGGHIAVESSLV
                     RGTTFHIYLPAFGKDVIHDNDLTETAEAVMKKKGKILVMDDAKSVRDVTGAMLSYMGY
                     DVEFAREGREAVAIYRAAKESGEPFDAVILDLTVSGGMGGKETIELLIALDPHIKAII
                     SSGFSGDPIMSEYQEYGFKTAILKPYKMEELEEVLERLIHDSDLKNL"
     gene            7379..7729
                     /locus_tag="PK125_09530"
     CDS             7379..7729
                     /locus_tag="PK125_09530"
                     /inference="COORDINATES: protein motif:HMM:NF018088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HPB38382.1"
                     /translation="MKITNLDSTIMTEVTMEGASKVKKQLPISSQDGSPTFSFRVFTV
                     EPGGHTPFHTHAFEHLNYIIDGEGAVVTQMGEEKPVKKGDFVLVLPGEKHQYKNKSSS
                     RPFVMICAVPKEYE"
     gene            complement(7769..9025)
                     /locus_tag="PK125_09535"
     CDS             complement(7769..9025)
                     /locus_tag="PK125_09535"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPB38383.1"
                     /translation="MTKTARYRWVIFWVLSCQYLIVYFHRVCPAVVAPELIKAFTISG
                     TSLGILASGYFYSYAITQIPVGFLSDSWGTRKTVTLFSSVAALGSILFAFAPNFGFAV
                     FARVLVGLGVSATFVSSLKTLAEWFSGREYARISGLLMAMGGIGWLTAATPLALLTEA
                     LGWRGAFVIIGIVTIFLALLTLFIVVDKPDSGSTTKVNRELVKSEHKLLINLSFVIRQ
                     KHFWVIAVWLFFTQGTLFSFFALWAGPYLIDTYNLSQSSAGNILSMIAVGMVFGSPFL
                     GYLSDRILASRKKVLIGASCVNVLVWCIMTAFYKEFSIPALFGVFFLMGITTSSIVVI
                     VFTAVKELFPLEMAGTAIGTANLFSFFGGVVFQPLVGYVLDMAGKIQGVYPPHAYQLA
                     FWVFLGMNTVSLVSIIFSKETMGKGE"
     gene            complement(9053..10075)
                     /locus_tag="PK125_09540"
     CDS             complement(9053..10075)
                     /locus_tag="PK125_09540"
                     /inference="COORDINATES: protein motif:HMM:NF027594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4922 domain-containing protein"
                     /protein_id="HPB38384.1"
                     /translation="MTPRILDPSNPSSKEQKTFAIFDGCESFSGLSKHCFMLLSEQRE
                     SWQELKDAYEALKTVKTRKIPLKDFSILLYFNPGRLISATAAVGKKDIQERPCFLCQH
                     NLPPEQKWILYRKEYRILCNPRPVLQSHFTISHVEHRPQAIPDCFDTFLNLMADLGPG
                     WITLYNGPRCGASAPDHLHFQALPTGHMPVEQELNKDKKFLQVARIAGISVYIANDLG
                     REMVVLKGDDPTSLREAFSKVFDALKEAIQTDDEPMVNIAGFYDKGSWLVVLFPRQKH
                     RPDVFYKEGDGRIIVSPGVVEMAGVLVTPMETDFERLDAATVESIYREVSLDNSILVN
                     AVENMR"
     gene            complement(10072..11550)
                     /locus_tag="PK125_09545"
     CDS             complement(10072..11550)
                     /locus_tag="PK125_09545"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HPB38385.1"
                     /translation="MKAVTVVAQYRTDPFFEKAVHLIEATGLVDRVLAVRRESASQKL
                     SAFCTPVDGEIHDRTTLMRIINETGTNHLLFLTGDGYVFFEPGSIEKMIAAAESTKAG
                     IVFSDFYDLNERGKTVHPLSGYQSGSVRDDFDFGGALFFSTEAIQKTLQKYGLIPDTR
                     YAGLYDLRLKVSIECPVHHIPEPLYSMISGKTHSGDGQLFAYVDPRNADVQKEMEIVF
                     TDYLKKIGAYLSPGSLRRAEQTQYRFPFTATVVIPVKNRVSTINGAVESALSQETDFP
                     FNVIVVDNHSMDGTTEILSGLAKRYPALKHIIPISTDLGIGGCWNEAINSKMCGRYAV
                     QLDSDDLYSSTKTLQRMVDALRDGKYAMVIGSYTIVDSHLKKITPGLVDHREWTDENG
                     HNNALRVNGLGAPRGFDTNIIRGIGFPNVSYGEDYAAALRVCREYRVGRIYDSLYLCR
                     RWEGNTDAVLTIEEANRNNAFKDSIRTEEIRARQQISKGPNP"
BASE COUNT         3314 a         2720 c         2491 g         3206 t
ORIGIN      
        1 gctttgttga atgcgttgcc agcagaaaaa cacaacagta aggcaaaaaa agcaaggatg
       61 acgtggcgga ataactttgc tattgccatc ggaaacctcc caaaaataat aagttctgcc
      121 ctttattatc ggcaaagatt gggattgttt ccgtgattct gctcacagcc gaaattgagc
      181 tgtgacctgc acttcacttc ctacatcctg gttctgttct ttttcaagat gaggtgttcc
      241 agaataagga ctgtcaagac acccacaaca aacccatttc caataatcgg cttcaggaaa
      301 atgggtagta aacgaaaaga agatgggggt agaaaagcaa tgatcgtacc cagaagagtt
      361 ggaattccta tggtcaagat attttgtact gaaaaatctt tctttgagtg aaaagcgata
      421 tctttaccag cggaaacctg aaaagacaga atatagatca ggctgcttcc aataacagca
      481 ggtggaatat tgtccaggag gttgattacg agaggcgaaa aagagatgag acagagcagg
      541 attgatgcag ggatcaaagc aaaacgggat gcgctttttg tggcccttat cacacccgga
      601 ctcagtgaaa aattaacagg acctataacc cccagaaatc ctgcaatgat atttgccacc
      661 ccggtaaatg taatacctct gtttattcga cttgacatac ctgtcgggtt cagtattcct
      721 tgcattgact ggatggagcc cagatcatta attgaaagag caaaaaaaca gaacagaaaa
      781 gaaacaagaa cgccagactc caaagaccat ctggtgatag gaccagtcag aaaaaatcct
      841 gcaaatctct gatcaattat tgcctctaag ttgatggcga aggggaagat aaggtaatac
      901 actgtgcttc cggcaatcat tgcccacaga atcattgtag atctccagag aggcccaaca
      961 agcctgtaag agagaaacat gcatccagtt aatatgatgg aaaaaataag attggcctcg
     1021 gccaggacag ccctgtgtgg ctttgttacc agatttaaga tcatgggtaa aagcgtaaag
     1081 gcaatcagaa tgagaactac agcaacaaca cggggggtaa ataatctctg aatataacca
     1141 aaaaggccgg atagactcaa tgcagttagc gctgctccac ctatcattat agacgtgtag
     1201 atgacgtcag cattgtagtt tctacctgaa ataataccta ttaatagaat ggtggaggga
     1261 ccaaggataa gcggaagcct gtggccccac aggatctggg caaagagggc aatagaaacc
     1321 atgaagcaca ttctctgaag ataggtaatc tggcccacaa ctgcactgtc atgaataatc
     1381 cctactactt ttcccacgat tattattccc gggattgata ttgcaagcca ttgaagaccc
     1441 agaagcagaa gttctccaaa tgatggccac tcatcgagct gatgtttcaa gtcaatgggt
     1501 ttcatacttt tttctacagt atcatttcta tcagaaatgg accagggtca gagagtgttt
     1561 ttgtcaattc ttttgcaagg tcttccgctg tactcactga tacagcagga acccccaaac
     1621 cggacgatat gcttacccaa tcaagttttg ggtggtctat atcggcaagg gataacgctt
     1681 ttgggcccgg atcagcaatc ccagccctct gcagctcaac cctgacaatg ttgtatcctc
     1741 gattggagca gatgagggtt tttacgttta acccctcccg agcctctgtc cacagggctt
     1801 gtaaggtata catagcactg ccatcagcct gaaaatttat aacagggcgg tcaggagatg
     1861 ccacggctgc ccccactgcg cagggcatgc catagccaat ggatccgcct acaatggtaa
     1921 gtactacatg agggggagtg aatgcagtta aaggataata gggtatgcca gaggtgatac
     1981 cctcatctac aatgattgcg ccttcaggtt gcagggcggc aagaacctgg catgcttttt
     2041 ccgctgtcaa tgttcccctg ggaagcaagg gcttaatgcc ctgtggcaaa ttccagcccc
     2101 ggttgctggc cgatgctgta ttcagttcat cttccaggca ttcaaggact tcaacaatgt
     2161 tttgtgtgcc ggagcaaata gagagttttt tttgatcttt tgtgagcaaa tggctgtcta
     2221 ctccgaaata cccgaagaag gtgacaggtt ccttagcatc agcgaggata attgtatcgt
     2281 actgagacaa gagcgcttgt gctggttcag gaaagtatgg aattctcatt acatcaggaa
     2341 gccctttgcc tctttcgaca tatccgggaa atgtttctgt gatgagatca catccagtgt
     2401 ttgactggat acgccctgct gttgaaaggc cctttccacg aagagctttg ccgcccagca
     2461 taaccgctgt tcgtctttct gttcgcaaca agtccgctgc agccttaatg agagtcatgt
     2521 caggggaatc aaatttgagt ggtgcaggag cacagatagt gtttcctgcc tcggcccaga
     2581 gatgatcgtt cggcacaata agggttgcaa tctttcctgg aagggattct aagtatgctt
     2641 ctgtaacagc actggagagt tcgttggcag aagtagtggt tttagtccag tcagagacag
     2701 cccttgcaag ggactcgaca tccatggtga gaggggcatc tgcatcaata tgccatgttg
     2761 tatgctggcc gataatattg atcaatggtg tacgtgccct ccgtgcattg tgaagattgg
     2821 caataccatt tgcaaagcca ggacctaaat gaaggagggt cagaccaggc ctctctttta
     2881 ctcgtccata tccatctgcc gcgccactac aaacgccctc gaaaagggcc ataacaggat
     2941 gtataccaga tacagtgtca aaggcagcaa caagtggaag ttcggttgta cctgcattgg
     3001 caaaacacac ttcaataccc gcagctgctg cagtctgtaa tataacttca gcgccattca
     3061 ttccttcttc tccttgacga gtaacaactg tcaatcctat cagaagtctt ttttatgtgt
     3121 caacatgaaa agcgcgaaga tatcctcctc tttgtgtctc tgatatgctt gagtctttaa
     3181 aggggggaga taaatttcga aggttgtact attataccat cactgaacac cctcaggaaa
     3241 ctggtatgct ggtggacaaa cccataaacc gcatgctgtc caatgccggt atcctaccgc
     3301 cattcaatgc agcgaaaaag gattagaata ttcttttgaa ctttcgtcca taccaatacc
     3361 agtatcagtg atagaaacgc gaacaaactg ccacgacctt ccaaaaccat ttttcgaaaa
     3421 atgtttcacc tgagttaacc agttctgtcg agatggtcag gatttcacca tcaggcatgg
     3481 catccgtaca ttgatggcaa gattcatgca agcctgtcct atctgtacca tatctaccag
     3541 aatagattgc ccacccttgc ccgttgcggt taccgaatca ctatcttccc cagtagtcct
     3601 tgaaagaaac ttgggtagaa aaagcgtttt cctctcatct atctctatgc ggaggtatca
     3661 ggttatgctg ccaaaagatt actcacagtg aggtttgtta tgagtttcat aagggtaaat
     3721 gcagattttg aggcaaaaca accattatca tactgttagg tgttttctgg ctttatgttt
     3781 caactatagt ctatttgcgg ggataacaga atattcctgg gtacatttca tttctttcac
     3841 gttgtttatt gttgtagacc ctatgaatta caccgtctaa caaaccacta ttgacacgaa
     3901 aacctctgat taaacatttg ggtcataaat tttttaaatc agaatcatga ataagccttt
     3961 caagaacttc ctcaagctct tccatcttat agggcttcag tatggctgtt ttaaatccgt
     4021 actcttggta ctcagacatg atggggtcgc ctgaaaatcc actggatatt atagctttta
     4081 tatgtggatc gagtgcaatg agcagttcta tagtctcctt gcctcccatc ccacccgaca
     4141 ctgtcaaatc aagaataaca gcatcgaagg gctcaccaga ctcttttgct gcccggtaga
     4201 tggctacagc ttcccttccc tcccgtgcaa attctacatc gtaccccata tagctcagca
     4261 tcgcaccggt aacatcccgt acggattttg catcatccat gacaaggatt ttccccttct
     4321 ttttcatcac tgcttcggca gtctctgtaa ggtcattgtc atggattacg tctttgccga
     4381 atgccggtag gtagatatga aaagtcgttc ccctgacaag gcttgattca actgcaatgt
     4441 ggcctccgtg ttttttgacg atggaatacg caattgccag tccaaggccg accccttttt
     4501 gactccctgc atcttttgtc gtgaaatatg gatcaaaaat ccgctcgagg tgttcttttt
     4561 ttatgccaga tccctgatcc tgaaatgaaa tctttacgta atccccttgc ttcaggggga
     4621 gatagtcttc acgactgacc ttcacattct caaaggatat tgcgacagta cctccttgag
     4681 gcatagcttc catggcattt cttaaaataa tatgcactac ctgccttatc tgcccctcgt
     4741 caacttccac agggaataga tcatcagaca tagtgtattt ccctctaata ttgctgttac
     4801 tcagggttga gcgtgttatc tccataacaa gggacgtaat taccatggcc ctctttgaaa
     4861 gttcccctcc ctttgaaaac gtgatgagct gctgaatcag gtcttttgtc atcatggagg
     4921 ccttttcagc atcaattaag tgttcaaacg ccttatcctc gcgggtcaaa cttgtcttgg
     4981 caagagatat attgcccagt ataacaagga gaaggttatt aaaatcgtgc gctatgccgg
     5041 ctgccagggt ttctactgac tcctgatttc tggacacagg ggcacgctcc ccgtaaacct
     5101 ttttcctggt aatgtcacgg atgagagata ccaccatatc atccattggg ataaaccaca
     5161 attcccgctc cagagatgca ccatcctttg ttgcaagttt gccctcaatt ggctgcaaga
     5221 tttcctctcc gatatttctt gtttttaccg cacgcaacca ggcattcatc gcaacatctc
     5281 tgagggattc gtcagaacaa actttttgca accaggcatc tattgactgg aaatcgtccc
     5341 gggaaaatcc aaagaccttt aaaaaacctt cgctcacata tttaatggtt ccttttcgat
     5401 cagatattat tgcagtatcg gatagcacct cgaaaagatt ttccaaaagc ttttcttgtt
     5461 gttttgagat atcctggctc tgaatgagtt gggtcatgtc ctgccctacc ccatacacaa
     5521 acctctcacc cgaaagcacc accggaacaa tgctgaacaa aaacctcttt gttttaccac
     5581 gtacttcgag ttgcgtttca aagctttcca tattgttcac cggtccctgg ctttcaaata
     5641 accgggcaag caattttttg ggaattaggt tgctggcatc cattttacga aactcttcca
     5701 gcgtacattc aaaaagctca accatttttt cattaaaatc actgtagcga tagtctctat
     5761 tgaccacaaa aaaagggctt ggtgcgcgtt gaaagagcgt cagatgtttc aggcgctcct
     5821 caaccagacc ggcttccgct tgcttcattt cagtaatttt tagcttgagg tcagtgttgg
     5881 atttcatcaa ctcttcagtg cgtgcttcaa tctttttttc atggtcacgc gagagtttgc
     5941 cgagcaattc ttcccgtcgt tttcgtgaca ggagatatag caccagaaag gttgctatga
     6001 tagcaaggct gaaaggaacg gcaataactt cccacttaat ctttttgtct tcacttggcc
     6061 ccggcttcgg ttcatttata atgatactgc cctccggcaa tgtatcctga ttaatgccaa
     6121 acttcattag ctgattataa tcaaacataa atatgttctg acttttatgc tctaccggta
     6181 tcctatcaac aggttcgccc tgcaaaatct taagccccat ctttgcagca agcatcccct
     6241 ggtgaaaacc gcttattagt ttgccgccaa tgatcccata tccgagatag tattccacca
     6301 atgcgtacag ggggatattg taccttgtaa ggatcggggt gctcacctcg ataggtaccg
     6361 ccgccccact actttcccta aatgatccca taaggataac tacaaagtct ttatccttat
     6421 ctttgagcag tttttcgaaa tcttctaaaa caggatcatc aataatagtt gcaatcagat
     6481 tttttcctgt tgctgcaagg gcatctttga aaatctgttt tttaacggca ccaatcgctg
     6541 taccatcatt aattgcaata atttctcttg tatttggatg cagttttagc gcaatatcca
     6601 gggttgcctt tatgtcaaaa tcttccagca ctcctgttac aagaggcgct ttttgccgca
     6661 tcatctcgtt ttgtgcgtcc acaccgcaga aaacaacagg tatcttggga aatagtaagt
     6721 ccctgtatct catagcaaag tttaaagcgt ctttattaga aactatgata agatcaaacc
     6781 gtactttttc aaatttggac cgatagtaca aaaagaggtg gtagaaatag tttttatcca
     6841 ccactctttt agtatccata aactctatgt gcagagtaat attgctttta ccatctttca
     6901 gaaccgattc tatacctgca agaacgttgt ccgtccaggg atacccttta tggtaagaat
     6961 taataatgag tacatgtctg ttttgatcgg gcggctgtgg aatcggaaca gcgttgctaa
     7021 ctgcgaaaaa gacaaggaaa catgctacac ttaatgaaag gtacagccct gttctcactg
     7081 atttctctct ccgcttaacc ttgtcttcat attgaaaata tccttgtttt acaaagactt
     7141 tactgattct tcgcataata ggaaacatgt cttaaattac atcacggcta tataatataa
     7201 caattcccat gacctgctgc aaaaacgaca tgtttgataa acagaaatta tgtggtagaa
     7261 atcttacgaa ttctgttaag ctatctctcg tctctttgct caaccctgat atgagaacag
     7321 tccctttgaa gttgaacatg ttcaagggga gtaataagca ttgtttgggg aggatgttat
     7381 gaagatcacc aatcttgaca gtacaataat gacagaggta accatggaag gagcaagtaa
     7441 ggtaaagaag cagctcccca tatcaagcca ggacggttcg ccaacatttt ctttcagggt
     7501 atttactgtt gagcctggcg gccacacacc tttccatacc catgcctttg aacatctaaa
     7561 ctatatcatc gatggcgaag gcgccgttgt aacacaaatg ggagaagaaa aaccggtgaa
     7621 gaaaggtgac tttgtgctcg ttctccccgg ggagaaacac cagtataaga acaaatcctc
     7681 ctcccggcct tttgtgatga tttgtgcagt accgaaagaa tatgaatagc ggggaagcaa
     7741 gatatgcaaa acccaaggta aatcgaattt actctccttt tcccatagtc tctttcgaaa
     7801 aaatgatact gaccagtgat actgtgttca ttcctaaaaa tacccaaaat gccaattgat
     7861 atgcatgggg tgggtagacc ccctgtatct ttccggccat atcaagtaca taaccaacga
     7921 gtggctgaaa taccactcct ccaaaaaaac taaagaggtt tgcagttcct attgctgttc
     7981 ctgccatctc cagtggaaaa agttctttca cagcagtaaa aactataaca actattgagc
     8041 ttgttgttat acccatgaga aaaaagacac cgaataaagc aggtatggaa aattctttat
     8101 agaatgcagt catgatgcac cagacaagaa cattcacaca tgaagctcca atgagcacct
     8161 tttttcgact tgcaaggatt ctgtcagaga ggtatccgag aaaaggactg ccgaatacca
     8221 taccgactgc aatcatagag aggatattcc cggcagaaga ttgagagaga ttgtaggtat
     8281 caataaggta tgggcctgcc caaagcgcga aaaaactgaa aagagtccct tgtgtgaaaa
     8341 aaagccagac tgcaataacc cagaaatgtt tctgcctgat gacaaatgat aagtttataa
     8401 gcaatttatg ttctgatttg acaagttctc gatttacctt cgttgtactg cctgaatccg
     8461 gtttgtcgac aactataaat aatgtaagga gcgccaagaa gatggttact atacctatta
     8521 taacaaaggc acctctccag cctaatgcct ctgtaagtag agcaagaggc gttgcagcgg
     8581 tgagccaccc aattcctccc atcgccataa gaagaccaga tattcgggca tactcacgac
     8641 cgctgaacca ttctgcaagt gttttcagtg acgatacaaa tgttgcagat acccccaagc
     8701 caacaaggac ccgtgcaaat acagcaaagc caaaattagg ggcaaaagca aacaggattg
     8761 accccagagc tgcaacagaa ctgaataggg taactgtctt tcttgttccc caggagtcag
     8821 agagaaaacc aactggtatc tgtgtaatgg cataggagta aaaatatccc gacgcaagga
     8881 tgccgagaga tgtgcccgat atggtgaaag cctttatgag ctcaggcgca acaacagccg
     8941 ggcataccct gtgaaaataa acaatgagat attggcacga cagtacccag aatatcaccc
     9001 agcggtaacg agctgttttt gtcaaaaaac accagccaaa gcttgatacc ccctacctca
     9061 tgttttcaac ggcattaaca agaatactgt tatccaatga tacttcccgg taaatcgatt
     9121 ctactgttgc tgcatccagt cgttcaaaat ccgtttccat tggggtaaca aggacaccgg
     9181 ccatctcaac cactcccgga ctgacaataa tccgcccgtc cccttcttta taaaaaacat
     9241 ctggacggtg cttttgtcgg gggaataata ccacaagcca cgatccttta tcgtagaaac
     9301 cggctatatt caccataggt tcatcatcag tctgaatggc ctccttgaga gcatcgaata
     9361 ccttcgaaaa ggcttctctc aatgatgtag ggtcatcacc cttgagcact accatctcac
     9421 gcccgagatc atttgcaatg taaacagaaa taccagcaat ccgtgcaacc tgtaaaaact
     9481 ttttatcttt attcagctcc tgttcaaccg gcatatgtcc tgtgggtaga gcctgaaaat
     9541 gaaggtgatc cggcgcagag gcaccacacc tgggtccatt ataaagagtt atccagccag
     9601 ggccaaggtc agccataagg ttgagaaagg tatcaaagca atctggaata gcctgggggc
     9661 ggtgctcaac atgagagata gtaaagtgag actgcaagac aggccttgga ttacaaagaa
     9721 tcctgtattc tttcctatag agtatccact tttgctctgg tggcaggttg tgttggcata
     9781 agaagcaggg gcgttcttga atatccttct tgcctactgc agcagttgca cttatcagtc
     9841 tgccgggatt gaaatagagg agaatggaaa agtcttttaa agggattttc ctcgtcttca
     9901 cagtctttaa ggcttcgtaa gcatccttga gttcctgcca tgattccctc tgttctgaca
     9961 agagcataaa acagtgtttg gaaagacctg agaagctttc gcagccatca aatatggcaa
    10021 aggttttctg ctctttggaa gaggggttcg aggggtcaag gattcgaggg gtcaagggtt
    10081 aggtccttta cttatctgct gacgagcacg tatctcttca gtgcggatag aatccttaaa
    10141 agcattattc cggtttgctt cttcgatggt caacaccgca tcagtatttc cttcccatcg
    10201 cctgcacagg tagaggctgt cgtaaatcct gccaaccctg tactcgcgac agaccctgag
    10261 ggctgccgcg taatcttctc cgtagcttac attgggaaac cctattcctc tgataatgtt
    10321 tgtatcaaac ccccttggtg ctccaaggcc gtttacgcgc agcgcgttgt tgtggccgtt
    10381 ttcatctgtc cattcacggt ggtcaaccaa gcctggcgta atcttcttta aatgggagtc
    10441 aacaatagta taggagccga tcaccatagc atacttccca tcacgaagcg catcaaccat
    10501 tctctggagg gtttttgtgc tgctatagag atcatctgag tcaagctgta ctgcatagcg
    10561 accgcacatt ttagagttga tagcctcatt ccagcaaccg ccgatgccca ggtcagtact
    10621 tatggggatg atgtgtttta gcgcgggata tcttttggca agacctgaga gaatctctgt
    10681 cgttccatcc atcgaatggt tatcaacaac gattacattg aaagggaaat ctgtttcttg
    10741 agagagcgca ctctctaccg ctccgttaat cgtgctgaca cggttcttga caggaataac
    10801 tacagtagcg gtgaacggga atcggtactg tgtctgttca gcccgcctca atgatccggg
    10861 agacaaatag gctcctattt ttttcaggta gtcagtaaat acaatttcca tctctttctg
    10921 aacatctgca ttccgggggt caacataggc aaagagttgt ccatcaccgg aatgtgtctt
    10981 tccagaaatc atcgagtaga ggggttcggg aatatgatgt acaggacact caatggaaac
    11041 cttcagtcgc aggtcataaa gaccggcata gcgtgtatcc ggtatgaggc catatttttg
    11101 aagcgtcttt tgaatcgcct ctgttgaaaa aaacaatgca ccaccaaagt caaaatcgtc
    11161 tcggacgctt cccgactgat aaccgctcaa tgggtgtact gttttccctc gctcattgag
    11221 gtcgtaaaaa tcagaaaaga caatccctgc ctttgtcgat tcagcggcag ctatcatctt
    11281 ttcaatgctg ccaggttcaa agaacacata cccgtcacct gttagaaaaa ggagatggtt
    11341 cgtgcctgtt tcgttgataa tacgcataag ggttgtcctg tcatgaatct caccatccac
    11401 aggcgtgcaa aaggcagaga gcttctggct ggctgactcc cggcgtacag ccaggaccct
    11461 gtctactaaa ccggtcgcct cgattaaatg cacagccttt tcaaaaaatg ggtcggtcct
    11521 atattgtgca acaacggtga cagctttcat ctgacaatat cctcattata tcaaaacaaa
    11581 acccggtaca tcagcatacc atggtttcag gtttaatcac tcgattttgt ctgaaaaaat
    11641 tatatcaagg ggggctttca gtgcgttggc catatttctc aacacacggg gataaatcat
    11701 ggtgatctct acattcttaa gcaccagaag g
//
LOCUS       DAOOMC010000088        11390 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdus sp. isolate AMR_MDS_2685
            NODE_6370_length_11390_cov_6.920953, whole genome shotgun sequence.
ACCESSION   DAOOMC010000088 DAOOMC010000000
VERSION     DAOOMC010000088.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864760
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdus sp. (wastewater metagenome)
  ORGANISM  Syntrophorhabdus sp.
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae; Syntrophorhabdus.
REFERENCE   1  (bases 1 to 11390)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11390)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,729
            Genes (coding)                    :: 2,708
            CDSs (with protein)               :: 2,708
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 7 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11390
                     /organism="Syntrophorhabdus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6370_length_11390_cov_6.920953"
                     /isolate="AMR_MDS_2685"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2699756"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..764
                     /locus_tag="PK125_09550"
     CDS             <1..764
                     /locus_tag="PK125_09550"
                     /inference="COORDINATES: protein motif:HMM:NF014101.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF134 domain-containing protein"
                     /protein_id="HPB38386.1"
                     /translation="MPRPTCPRRVGFTPSTTYFKPAGTPIDILEEVLLGHDELEAIRL
                     KNLVGLSQEQASLQMGISQSTFHRLLVSAHQKLSDAVVNGKALRIEGGNVTTPDVSVG
                     VCRWKNEWSCKIKTSVSICSEDNNRQVEEKRGMKIAITSTDGTLQGKVDLRFGRAQKI
                     ILYDMETKKHTVVNNVQNLNAAQGAGIQTAQNVVSLGARAIISGHFGPKAFQAIQMAG
                     LDVYSATNISVEEAIRQFEIGKLIKLANPDVEPHW"
     gene            847..1578
                     /locus_tag="PK125_09555"
     CDS             847..1578
                     /locus_tag="PK125_09555"
                     /inference="COORDINATES: protein motif:HMM:NF015949.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF364 domain-containing protein"
                     /protein_id="HPB38387.1"
                     /translation="MKILTDILSSINDDTLVDDVVRGLYWTAVTSSSCGLSSTMFRDC
                     AHDHSDDVEPVVLIGQPAADIARFSLSPDIGMASLGLAAINSLIPIDLSQCVEINAGK
                     IIMDKGRGKNISIIGHFPFVDDLKDVAKNLWVIEKWQRPGDYPAEDASKYLPDSDVIA
                     ISSTTLINHTLEDLLPLCPTSSFTMLLGPTTPMTPVVFDHGIDMISGSLVIDKQLALT
                     CIKQGANFRQLKRSGAIRLLSLQKS"
     gene            1603..2010
                     /locus_tag="PK125_09560"
     CDS             1603..2010
                     /locus_tag="PK125_09560"
                     /inference="COORDINATES: protein motif:HMM:NF013738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly scaffold protein"
                     /protein_id="HPB38388.1"
                     /translation="MSFYSEKVVSHFKEPKNVGFMEDADGIGEIGDPSCGDFMRVFIR
                     VRDNTIADVKYQIRGCPASIAVASVMTELAIGKDLDDALMLTDDDVVKALDGLPEEKL
                     HCSVLGITGLRKAIQDHFMRYIGDVKPQEDQNG"
     gene            2476..3003
                     /locus_tag="PK125_09565"
     CDS             2476..3003
                     /locus_tag="PK125_09565"
                     /inference="COORDINATES: protein motif:HMM:NF012360.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HPB38389.1"
                     /translation="MLYRFDGKEPVIGSGTYVSETATVIGNVTIGNDCYIGHGAILRG
                     DYGRIEIGDGTAVEEGVIIHAPPEDICSVGRGVIIGHGAIIHAKSIGDLAAIGMGAIL
                     SIRSEVAGRSIVAEGAIVKQNQKIPESTMVAGNPARQIRKALQKEQEHWSWARELYVD
                     LAHKYCDLGMEKIAC"
     gene            complement(3087..3629)
                     /locus_tag="PK125_09570"
     CDS             complement(3087..3629)
                     /locus_tag="PK125_09570"
                     /inference="COORDINATES: protein motif:HMM:NF025890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HPB38390.1"
                     /translation="MIQTKCRKSREMAKAKFFIALFVPLFFLAILVSTGLSAPKKVST
                     AKPGDCAACHESKRVLPPDHPDTKQMGLSACSPCHQKMGESSLRTKMPVSHAHNLAGV
                     TCEKCHGKAQKRQAVEMAKCITCHNPAKLVEKTAKIKPENPHTSPHYGDSLDCNLCHH
                     QHEKSENYCNQCHQFNFNVP"
     gene            complement(3755..5344)
                     /locus_tag="PK125_09575"
     CDS             complement(3755..5344)
                     /locus_tag="PK125_09575"
                     /inference="COORDINATES: protein motif:HMM:TIGR01813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavocytochrome c"
                     /protein_id="HPB38391.1"
                     /translation="MKKNNGKEKKAKLEQKTEHGSVSRRGFLKTGLGLGALATASGLA
                     LNLTKKGAHAAQFKPMPKKWDETYDVVVIGSGFAGLSAAIEAKNAGAKVTVIEKMPVH
                     GGNSIINGGDFCAPDTKLQKAAGVKDSPVLMYADMMKAGQYLNHPELARIVANQSRDA
                     LEWSESYVGAQFTRLNYHGGHSVKRANQTINASGSELVNKMFAKIKELGVNVQTRTKL
                     VSFITNKDGRIVGIEVLKGYRFPDEKSGKVAYIKAARAVVLTSGGFSRDLALRQVEDP
                     RLTDKFESTNQPGATGEALLAACMAGAMNVQMDWIQLGPWTSPDEKGFGHVPLFCERL
                     VGYGPMIDPKTGKRFFKETGNRKERADAIILLGHPVIILGDSYAVPKQVFPNALQKGI
                     EAGAIKKFNTLEDLAKNYDIPVDAFRQEMARWNSFVEKKKDDDFDCMIFPDAKPTVTG
                     PFYAARLWPKVHHTMGGLVIDKNAQVIGFDFKPVKGLYAAGEVTGGVHGAVRLGGVAM
                     ADCIVFGRIAGQNAAKEKAWG"
     gene            complement(5374..5655)
                     /locus_tag="PK125_09580"
     CDS             complement(5374..5655)
                     /locus_tag="PK125_09580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB38392.1"
                     /translation="MRWMEMIRLRTLQGREKKLEKLLADSAHAAIKEPGLEEARVYAS
                     ASFYSDLALNLLWDTDMIHHQGSRVGLSISQTLASYGLVEHSIWSEKVV"
     gene            5858..7399
                     /locus_tag="PK125_09585"
     CDS             5858..7399
                     /locus_tag="PK125_09585"
                     /inference="COORDINATES: protein motif:HMM:NF012385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HPB38393.1"
                     /translation="MIDEKTFFSETTLRICGSLDIEKALFNCFLYVRDVIPVDELNLA
                     VYDSALGALEIVATADRNGGLARSDKIMMPPHLRVQLESAIKYPRVRMMDDIFQDEIV
                     RLVAQKYGWPDSSIIIGRLIVEGKFLGSLVTRAIGKGRYTEDHARLWSLVNEPVAIAL
                     TNSRRYLQLMVLNELFADDSKYFQEELRKPFGEEIVGSQFGLREVMNHVTRVASLPSP
                     VLLVGETGTGKEVIANAIHNLSSRNNGPLIKVNCGAIPESLIDSELFGHEKGAFTGAF
                     MQKRGRFERAHGGTIFLDEVGELPPQAQVRLLRVLQEKEIERVGGAHPVKVDIRIISA
                     THKNLEQQVVRGLFRDDLYYRLSVFPIRIPPLRERKADIPALVEFFIRKKAKEIGLQS
                     IPQLTPGAMDQLMAYYWPGNVREVGNVVERALILSGGSILRFDDILGPRHKLFGSEDQ
                     LTEEPLQLNEVEANHILKVLKMTGGRVEGKKGAAQLLGMHPGTLRHRMKKLKVPFGRA
                     SKISR"
     gene            7532..7732
                     /locus_tag="PK125_09590"
     CDS             7532..7732
                     /locus_tag="PK125_09590"
                     /inference="COORDINATES: protein motif:HMM:NF011430.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HPB38394.1"
                     /translation="MFGLGMPELIVIMVIILLIFGAGKLPEVGGAIGKAIKSFKKSVK
                     EPDEIDITPKDDKKEEKPPEKT"
     gene            7827..8825
                     /locus_tag="PK125_09595"
     CDS             7827..8825
                     /locus_tag="PK125_09595"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: protein motif:HMM:TIGR00057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HPB38395.1"
                     /translation="MNILNGFESGAIEQAVAVLTAGGVVAFPTETVYGLGADAFNPYA
                     VTKIFEIKRRPRFDPLIVHIYSKDWMFDIAEHVPVLATHLADRFWPGPLTIILRKKSI
                     IPGIVTAGLSTVGIRMPSHPVALELIKTLGRPVAAPSANPFGYISPTRAKHVIKSFKD
                     KVRIVLDGGDSQFGIESTIVSIAADDDKVIVHRHGAIPVEEIQAMGITIEEKKGNDTC
                     EAPGELPYHYAPVKPLIIIDTFEESLLESSSFLGFIEKKEKPTVRYIKYLSQDGDLRE
                     AAANFFSHLIDLDREDISVIYAEKIPDKGLGKAMMDRLKKAAQKCVLQGVEKYDGS"
     gene            8949..9440
                     /locus_tag="PK125_09600"
     CDS             8949..9440
                     /locus_tag="PK125_09600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB38396.1"
                     /translation="MLNIYPDNFFTGFPQKMWKTIGSPIVMKVCLIILSVALLGSCSY
                     LKGGKKQDDRAVANPSAAETSKQTDQKPPGKQQTTAPEKKAPLLSKEPPKAKLVPDSG
                     LIDMTEAEVKKKFGEPDIVSKMPDNRILWTYQPTWKLLPDNKGTVYIEFENGKVVKIN
                     RAR"
     gene            9825..10328
                     /locus_tag="PK125_09605"
     CDS             9825..10328
                     /locus_tag="PK125_09605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB38397.1"
                     /translation="MFYGEKALRSGYYDEAKQNFEEAYEKNKTPEALMYLAMVDYKTN
                     NLDSAESLVREAEWMGSVNYHYLRVLGYKALILLKKNSDEGLEALDQYVGFYASCDPL
                     MSIQEVRRMAQTSNIDMPLLEKLIEEQVSWFENDVELYWSSGVGYYDARSFFGGSFRF
                     HGGGIFH"
     gene            10340..11272
                     /gene="ftsY"
                     /locus_tag="PK125_09610"
     CDS             10340..11272
                     /gene="ftsY"
                     /locus_tag="PK125_09610"
                     /inference="COORDINATES: protein motif:HMM:TIGR00064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HPB38398.1"
                     /translation="MGIFDKIRNGLAKTRDQLFTRIEWVVKGKPIDEDAFDEIEEALI
                     LADVGFETTQVFMTALKDRWKRGQIRISEDIGQAMREEIETFLTPVEASIPLNSTKPF
                     VILTLGVNGVGKTTTIAKLANIYLNSGKKVILGACDTFRAAAVEQLEVWAKRMGIDVI
                     KHAEGSDPAAVAFDATKAAKARGTDLLILDTAGRLHTKANLMEEMKKIKRVVGKELDG
                     APHEVLLTIDATNGQNAIAQARTFNESLQITGLVITKLDGTAKGGFVLPIAHLLKIPI
                     RYIGVGEGIDDLIPFTAKDFATALISPESPSSSL"
BASE COUNT         3317 a         2388 c         2639 g         3046 t
ORIGIN      
        1 ggatgccaag accaacatgc cccagaagag ttggcttcac cccaagcaca acatatttta
       61 aaccagcagg aacacccata gacatactcg aagaggtgct tttaggccat gacgagcttg
      121 aagcaattcg gctgaaaaat ctggttggtc tctctcagga acaagcatca ttacagatgg
      181 gtatatctca gtcaacattc catcggcttc tcgtctctgc tcatcaaaaa ctgagtgatg
      241 cagtggtgaa tggcaaagct ttacggattg aaggcggcaa tgtaacaacc cctgatgtgt
      301 cagtgggagt atgcagatgg aagaatgaat ggagctgcaa aataaaaacc tccgtatcca
      361 tatgttcaga ggacaacaat cggcaagttg aagaaaaaag aggtatgaaa atagcaatca
      421 cgagcactga cggtacactc cagggaaagg ttgacctgcg ttttggaagg gcacaaaaaa
      481 ttattcttta tgacatggag acaaagaagc atactgtggt aaataatgtt cagaacctga
      541 acgcggccca aggggccggt attcagacag cccagaatgt ggttagttta ggggcgagag
      601 caatcatcag tggtcacttt gggccaaaag cattccaggc aatccagatg gcaggactcg
      661 atgtatattc tgcaaccaat atatcggttg aagaggcaat tcgacaattt gagataggta
      721 aacttataaa acttgcaaac cccgacgtgg aaccgcactg gtaatgagaa tagatataac
      781 actcgcaata cttgtgaaac attataaagt aacacagggc ggacagggtg aatacgggag
      841 attaccatga aaatactgac tgacatctta tcttcaatca atgatgatac acttgttgat
      901 gatgtagttc gcggtcttta ctggacagct gttacgagca gttcatgcgg gctttcttcc
      961 actatgttcc gtgactgtgc ccacgatcac tctgatgatg ttgagccggt tgtcttgatc
     1021 ggacagcccg cagccgatat tgcaagattc tctctttccc cggacatcgg aatggcttcg
     1081 ttgggtttgg cagcaataaa ttcacttata ccaatcgatt tgtcacaatg tgtcgagata
     1141 aatgcaggca aaatcattat ggataaaggc aggggcaaga acatctccat tattggacac
     1201 ttcccttttg tagatgatct aaaagatgtt gcgaaaaacc tttgggttat tgaaaaatgg
     1261 caaagacctg gcgattaccc tgcagaagat gcgtccaagt atcttccgga ctctgatgtg
     1321 atagccatct caagcaccac gcttatcaac cacacgctgg aagatctatt gcccctgtgc
     1381 cccacatcca gctttacaat gctgctggga cctacaacgc ctatgactcc tgtcgtattc
     1441 gatcacggca ttgacatgat atcaggaagc ttggtcatcg ataagcagct tgccctcacg
     1501 tgcattaagc aaggggcaaa cttcaggcaa cttaaaaggt ctggtgcaat aaggctcctc
     1561 tcattacaaa aatcttagat ttatcaggag gttgtaaaaa ccatgtcttt ttacagcgaa
     1621 aaagtagtat cccacttcaa agaaccgaaa aatgtcggtt tcatggaaga tgcagatggg
     1681 ataggagaaa ttggtgaccc ttcatgtggt gattttatga gagtttttat tagggtacgt
     1741 gataacacga ttgctgatgt gaaataccag atcagaggct gtcccgcttc cattgctgtg
     1801 gcaagtgtca tgaccgaact tgctatagga aaggatttgg atgatgctct catgctcaca
     1861 gatgacgatg tggtcaaggc cctagatggc cttcccgaag aaaaactcca ctgttctgta
     1921 cttggtatca cgggtttgcg aaaggcaatc caggaccact ttatgcgcta tataggagat
     1981 gtaaagccac aagaggatca gaacggatga aagcgccctt gattatatga agcttacccc
     2041 agttaaacaa ggtcatggac gagaagaaag gcatacattc taaatttttg ggaaatgcct
     2101 tttatatatt gggtattttt tcacaaacaa gcacgggcct accatgaaga aagcacacaa
     2161 ctaccagaac atggatattg ataaggatac ccggattgtc cacaatgaca gaatgttccg
     2221 aatgggtagg gatcgttttg tgtgccacac ttaatgattg gttatctcat ggcccatgac
     2281 cgtttggtga gagtgataga acagcgacca gtttgatcgc acaaatcttc agaaacataa
     2341 tgaaacggaa cacccattcg aatagacaat gtgaaacgat aaaactggta acccaagaat
     2401 tactaaaggg caagaaagaa gattgatcga atcacgaaga tacaaactga aaagatcaag
     2461 tggaggaaat aagaaatgct ttacagattt gatggaaaag aacctgttat cggatctggt
     2521 acgtatgtga gcgaaacagc cacggtcatc ggtaacgtaa caatcggaaa tgattgctac
     2581 atcggtcatg gtgcaattct acggggagac tacggaagga tcgagatagg ggatggaaca
     2641 gcagtggagg aaggagtgat aattcacgcc cctcctgaag atatctgttc tgttggccgt
     2701 ggggtcatca ttggccatgg agcgatcata cacgccaaat ctataggtga tttggctgca
     2761 ataggcatgg gggcaatatt aagcatccgg tccgaggttg cagggagatc cattgtagca
     2821 gaaggagcca tagtaaaaca aaaccagaag ataccagagt caaccatggt tgctggcaac
     2881 cccgcgagac aaatccggaa agcattgcaa aaggaacaag aacactggag ctgggcaaga
     2941 gagctctatg tagacctggc tcataagtat tgtgaccttg gcatggagaa aatcgcttgt
     3001 taggggcatg atacatttca agatttgcta gcttttccca gaacaactgc ttgtaggaat
     3061 aggccggagg atttcactga acatgctcat ggtacattaa aattgaattg atggcactgg
     3121 ttgcagtagt tttctgattt ctcatgctgg tggtgacaaa gattacaatc gaggctgtct
     3181 ccataatgtg gggacgtgtg ggggttctca ggttttatct ttgctgtttt ttcgacaagc
     3241 tttgcaggat tgtggcaggt tatgcacttt gccatttcaa cagcctgtcg cttctgtgcc
     3301 ttcccatgac atttttcaca tgtcacacct gcaagattgt gggcgtgact aaccggcatc
     3361 tttgtccgta aactgctctc acccatcttt tgatgacagg gcgaacaggc cgaaagaccc
     3421 atttgtttgg tgtcagggtg gtcaggggga agtacccttt tgctttcatg acaggctgcg
     3481 cagtcacctg gcttggctgt tgaaactttt ttcggtgccg atagcccagt agaaacgaga
     3541 atagcaagga aaaacagggg aacaaaaaga gcaatgaaaa atttagcttt tgccatttct
     3601 cttgatttcc gacactttgt ttgtatcatt gcatacctcc ccactcattt caatctatag
     3661 gcgttgagaa acaatattca gtatttaaca gggaagagtg tggttttcac cacaactcct
     3721 ccctggactt aaatactcgg tgttgctggt ccaattaacc ccaagccttt tcctttgccg
     3781 cattctgacc ggctatccgt ccaaaaacta tacagtcagc catggctaca ccaccgaggc
     3841 ggaccgctcc atgtaccccg cctgttacct ctcctgcagc atagaggcct ttgactggtt
     3901 taaaatcaaa gccaattacc tgggcgtttt tatcaataac caggcctccc atggtgtgat
     3961 gaactttggg ccataacctg gcagcataaa aaggccctgt tactgtgggc ttggcatcag
     4021 gaaagatcat gcagtcaaaa tcatcatcct ttttcttttc aacaaaagag ttccagcgtg
     4081 ccatttcttg ccgaaaagca tcaacgggta tatcatagtt tttagccagg tcttcaaggg
     4141 tattgaattt tttaatagcc ccagcctcaa tacctttttg aagtgcattt ggaaatacct
     4201 gtttgggtac agcataggaa tcgccgagaa taatgaccgg atgtccaagg aggataatag
     4261 catctgcgcg ctccttgcga ttcccagttt ctttgaagaa tcgtttgcct gtcttgggat
     4321 ctatcatggg cccgtaaccg acaagcctct cacaaaacag gggaacatga ccgaagccct
     4381 tttcgtcagg acttgtccag ggtccaagct ggatccagtc catctgaaca ttcatggcac
     4441 cagccataca agctgcaagc aatgcctcac ccgttgcgcc tggttggttg gttgattcga
     4501 atttatcagt gaggcgggga tcttctacct ggcgcaatgc aaggtctcga gaaaaaccac
     4561 cagaagtcag gacaactgcc cttgcagcct taatgtaagc caccttgccg gatttctcgt
     4621 ctggaaaacg atatcctttc agcacctcta ttccgacgat tctaccgtct ttattggtaa
     4681 taaagcttac aagttttgtt ctggtttgaa cattcacgcc aagttccttg atttttgcga
     4741 acattttgtt gacaagttca gacccggatg cattaatggt ctggttggct ctttttactg
     4801 aatggccgcc atgatagttg agccgggtaa actgggcgcc cacatagctt tcagaccatt
     4861 caagggcatc tcttgattga ttggcaacaa tccttgccag ttctgggtgg ttcaggtatt
     4921 gccccgcttt catcatatct gcatacataa gaacaggaga gtctttcacc cccgctgcct
     4981 tctgtagctt tgtgtcagga gcacaaaaat caccaccatt gataatagag ttgccaccgt
     5041 gaaccggcat cttctcgata acagtaactt ttgcacctgc gttttttgcc tcaatagcag
     5101 cagacagacc ggcaaaccca ctgccaatta caactacgtc atatgtttcg tcccattttt
     5161 tgggcatcgg tttaaattgt gctgcgtgtg cccctttctt tgtaaggttg agggctagac
     5221 cgctcgctgt tgcaagagcg ccaaggccaa gccctgtctt gagaaaacca cgtctgctga
     5281 cactgccgtg ttctgttttt tgttcaagct ttgccttttt ttcttttcca ttattctttt
     5341 tcatttgtat cctccttcct gaattacagg tttttaaact accttttcag accaaatcga
     5401 atgctctacc agcccgtaag atgctaaagt ttgagatatg cttaatccca ccctgcttcc
     5461 ttgatggtga atcatatcgg tatcccaaag aagattaagg gcaagatcgc tgtaaaatga
     5521 tgcgctggca tatacccttg cctcttctaa accaggttcc ttgatggcgg catgtgccga
     5581 gtcagctagc agtttttcca gttttttttc ccgtccctgt agtgttctta accgtatcat
     5641 ttccatccag cgcatgtctg cttatccttc tctttttata aactaaccat aaaaaagcac
     5701 aaaccgtacc acgacaaata gtcatataat accggttagt tatgaatgca aagcaaaaaa
     5761 actaccatat ttagcagata ttaactacca tatttagcag aattatatta aactaccata
     5821 tttagtagtt taatatgcat taagaaattt atagcttatg atagatgaaa aaacgttttt
     5881 cagcgaaaca accttaagaa tttgtggaag ccttgacatt gaaaaagctc tctttaactg
     5941 cttcctctat gtgagagatg taattcctgt agatgaactt aatcttgctg tttatgattc
     6001 tgctttaggg gcgctggaaa ttgttgcaac agcagacagg aatggcggac ttgcgcgctc
     6061 tgacaagata atgatgccgc cgcaccttcg tgtgcaactt gagtcagcaa ttaaatatcc
     6121 ccgggtaaga atgatggatg atatatttca agatgaaatt gtaagactcg ttgcacaaaa
     6181 atacggttgg cctgactcgt ccataatcat cgggcggctc attgtagaag gaaaatttct
     6241 tggttccctt gtcactcgcg caatagggaa gggcagatat actgaagatc atgcccggct
     6301 ctggtctctt gtgaatgaac cagttgctat tgccctcaca aatagtcggc gatacctcca
     6361 actcatggta ctgaacgaac tttttgcaga tgacagcaag tattttcaag aagaactccg
     6421 aaaacccttt ggggaggaga ttgtaggaag ccagtttgga ctcagagaag ttatgaacca
     6481 tgtaacgagg gttgcatctc ttcccagtcc ggttcttctc gttggtgaga ccggtactgg
     6541 caaagaggtt attgcaaatg caattcataa tctatcatct cggaacaacg gacctctcat
     6601 taaagtaaac tgtggagcca tacctgagtc tcttatagat agcgagcttt ttggtcatga
     6661 aaaaggcgcc tttaccggtg cgtttatgca gaaaagaggt cgctttgaaa gggctcatgg
     6721 cgggacaatc ttccttgatg aggtcggcga gcttcctcct caagcacagg tgagactttt
     6781 aagagtcctt caggaaaagg aaattgaacg ggtcggaggt gctcatcctg taaaagttga
     6841 tatccgcatc atatctgcaa cccataagaa ccttgagcaa caagtagtac gtggtttatt
     6901 ccgggatgat ctgtattacc ggcttagtgt gtttcccatt cgcattccac cccttagaga
     6961 gagaaaggcc gatattccag cattagttga atttttcata agaaaaaagg ctaaagaaat
     7021 agggcttcag tctatccccc aattgacccc gggcgccatg gatcagctca tggcgtatta
     7081 ctggcccggt aatgtccggg aagttggaaa cgtcgttgaa agggcactta ttctgtcggg
     7141 aggcagtatt cttcggttcg atgatattct tggcccgagg cataagttgt ttgggtcaga
     7201 agaccaatta acagaagaac cccttcaatt aaatgaagta gaagctaatc atattttgaa
     7261 agtcctcaaa atgacagggg gcagggttga aggaaagaaa ggtgctgctc aactgctggg
     7321 gatgcatcca ggcacattgc gacacagaat gaaaaaactc aaggtgccct ttggcagggc
     7381 ctcgaaaatt tcccgataat catagcttca atttgttttg ttgggctaaa aaatgtatag
     7441 cgacctgtct gtctggcgag tgacaaaaca agacaacggg tctgttgact aaaatattga
     7501 aaacgtgata tttttagtaa ggaggtgcaa catgttcggt cttggaatgc ctgagcttat
     7561 tgtaataatg gtaatcatcc tcctcatttt tggcgcagga aagctgcccg aagttggggg
     7621 cgcaatagga aaagcaatta aaagttttaa gaaatctgta aaagaaccgg acgagataga
     7681 catcacgccg aaggatgata aaaaagagga aaaaccacct gagaaaacgt aatgttcttg
     7741 agcgtttggt tttcttactt tacccatcat agcataatat aattccccac catggttaag
     7801 ctaaataaac agacacattt gtttcgatga atattctgaa tggttttgaa tctggagcaa
     7861 ttgaacaggc agttgctgtt cttacagcgg gaggggttgt tgcttttcca actgagacag
     7921 tttacggcct tggtgcggat gcgtttaacc cttatgcagt gaccaagata tttgaaataa
     7981 aaagacggcc aaggtttgac ccgttaattg ttcatattta tagcaaagac tggatgtttg
     8041 acatagcaga gcatgtcccg gtactcgcaa cacatttggc agacagattc tggccaggac
     8101 ccctgactat tattctcaga aaaaaaagca ttattcctgg aatcgtaaca gcgggccttt
     8161 cgacagtagg tatcaggatg ccttcgcatc cagttgcgct tgaactcata aagaccctgg
     8221 ggcgacctgt agctgcgcca agcgccaatc catttggata catcagtcct acaagggcta
     8281 agcatgtgat aaaatccttt aaagacaaag tacgaattgt gttggacgga ggagacagcc
     8341 agtttggaat agaatccacc atagtctcta ttgctgctga tgatgataag gttatagtgc
     8401 acaggcatgg tgccattcca gttgaggaga tccaggcaat gggcataacc atagaggaaa
     8461 agaaaggcaa tgatacatgt gaagcgcctg gagaattacc ctatcactat gcgcctgtaa
     8521 aacctctaat aataatagat acttttgaag agagtttact cgaaagctct tcttttctcg
     8581 gtttcattga gaaaaaggaa aaaccgacgg tacgttatat aaaatacctt tcgcaggacg
     8641 gggatttgcg agaagctgcc gcaaattttt tttcccatct tatcgattta gacagggaag
     8701 atatttctgt gatctatgca gaaaagattc ctgataaagg tttgggaaag gccatgatgg
     8761 acagactcaa gaaggctgct caaaagtgtg ttctacaagg cgttgaaaaa tatgatggtt
     8821 catgatagcc tacacgatat gctgcaacct attcggcggt accgtgcttc tgagattgtt
     8881 cttgataata ttaggctttt tcttccccca tattttttga aattggacta aaggagttta
     8941 atgtaactat gttaaacatc tatcctgaca attttttcac aggtttccca caaaaaatgt
     9001 ggaaaactat aggaagcccc atagttatga aggtttgtct gatcatactc agcgttgctt
     9061 tattgggttc atgctcatac ctcaagggcg gtaagaaaca ggacgaccga gcagttgcga
     9121 atccttcagc agctgaaacc tccaaacaga ctgaccaaaa accgccagga aaacaacaga
     9181 ccaccgcccc ggaaaagaaa gctcctctgc tatcaaaaga accacctaag gcaaaattgg
     9241 tccctgacag cggcctgatc gacatgacag aggctgaggt gaaaaagaag tttggagagc
     9301 ccgatattgt tagcaagatg ccggacaacc gtattctctg gacataccag ccgacatgga
     9361 aattactccc tgataataaa ggcactgtgt atatagagtt tgaaaatggc aaagttgtta
     9421 aaataaatag agcaaggtga cctatgatct gcgagaagtg tggttcagag gtagaaacag
     9481 tgaaatgtgt tcattgtggg caagaagtaa tcaggctcgg gccacattgt tatcactgtg
     9541 gtaaagagtt acatgttcat gcagaaggag aaactgacaa cactgacttt gacaacagga
     9601 tactttgtag cgatggtgct tgtatcgggg tcattaacga acacgggata tgcaaggtat
     9661 gtggaaagcc ctatacaccc gaagtatgaa aacctgtgat atgtcatgag agtttaaaat
     9721 tgttaaatta catcagtaag tccattgttt tcgttttttg ttacattatg ctgttcttgt
     9781 cgcttagcgc ctgttccact atacatcatg cgtacagcac aaatatgttc tacggtgaga
     9841 aagcactcag aagcggttat tatgatgagg caaagcagaa ctttgaggag gcctatgaga
     9901 aaaacaagac accggaggcg ctcatgtatt tggcaatggt ggactacaag acgaacaacc
     9961 tggatagtgc ggaaagcctg gttcgagagg cagagtggat gggaagtgtg aattatcatt
    10021 atcttcgagt tcttggctat aaggctctta tcctattaaa aaagaacagt gacgaaggac
    10081 tggaggcact cgatcaatac gttggctttt atgcgtcctg tgaccctctc atgtcgatcc
    10141 aggaggtccg gagaatggca caaaccagta atattgatat gccactcctt gaaaaactca
    10201 tcgaagaaca ggtttcttgg tttgaaaatg atgttgaact atattggagt tctggtgttg
    10261 gctattatga tgccagaagt ttttttggtg gatcttttcg atttcatggg ggcggtattt
    10321 ttcattaaac tgtaaaataa tgggaatttt tgacaaaata agaaacgggc tagccaagac
    10381 aagagatcaa ctttttacaa gaatagagtg ggttgtaaag ggcaagccca tcgatgagga
    10441 tgccttcgat gaaattgaag aggcactgat ccttgccgat gtagggtttg agactacaca
    10501 ggtttttatg acagctttga aggataggtg gaaaagaggc cagatcagga tatcggaaga
    10561 tatcggtcag gcaatgagag aagagataga gactttcctc acgcctgtag aagcatcaat
    10621 tcccctgaac agtacaaagc cttttgtaat tttaaccctt ggtgtcaatg gggttggaaa
    10681 aacaaccaca attgcaaaac tggcaaacat atacctcaac tccggaaaaa aagttattct
    10741 gggtgcatgc gatacattca gggctgctgc tgtggagcaa ctcgaggttt gggcaaaaag
    10801 aatgggaatt gatgtcataa agcacgccga gggatctgac ccggcagcgg ttgcctttga
    10861 tgccacaaaa gctgccaaag cccgtggaac agaccttttg attcttgata ctgcagggag
    10921 attgcataca aaggccaatc tcatggaaga aatgaaaaag attaaaagag ttgttgggaa
    10981 agaacttgat ggagcccctc acgaggttct tttgacaatc gacgcaacga atggtcagaa
    11041 tgccatagca caggcaagaa cctttaatga atccttacaa ataactggac tcgttattac
    11101 gaaattggac ggaacagcta aaggaggctt tgtcctgccc atagcccact tacttaaaat
    11161 accaatacgg tacattggcg tcggggaagg catagatgac cttatccctt tcactgcaaa
    11221 agactttgcc acagccctca tttctcctga atcaccgtca tcctctttat aagcatatct
    11281 ttatccgagt cttggccacc cggcatctgc aatgtgccaa ggatgctgtc tgctaatagc
    11341 caccccttga ttctggaacc tttttccagt ttgactttgc accggttcat
//
LOCUS       DAOOMC010000089        11353 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdus sp. isolate AMR_MDS_2685
            NODE_6410_length_11353_cov_6.637812, whole genome shotgun sequence.
ACCESSION   DAOOMC010000089 DAOOMC010000000
VERSION     DAOOMC010000089.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864760
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdus sp. (wastewater metagenome)
  ORGANISM  Syntrophorhabdus sp.
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae; Syntrophorhabdus.
REFERENCE   1  (bases 1 to 11353)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11353)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,729
            Genes (coding)                    :: 2,708
            CDSs (with protein)               :: 2,708
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 7 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11353
                     /organism="Syntrophorhabdus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6410_length_11353_cov_6.637812"
                     /isolate="AMR_MDS_2685"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2699756"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1682
                     /gene="bamA"
                     /locus_tag="PK125_09615"
     CDS             <1..1682
                     /gene="bamA"
                     /locus_tag="PK125_09615"
                     /inference="COORDINATES: protein motif:HMM:TIGR03303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HPB38399.1"
                     /translation="SKLKSTMMVREKGFFSWFTGSGILEEDALDDDRKRLESFYSDNG
                     YIKAKIGVPDISISKDGRTISVVIPIEEGAIYRIGKIDFAGDLIFPVDVLNKDLKSKP
                     GNIFRSSQYREDVIMLTDLYQDRGYAFTDISPVTLVDDSTQKIDLTFNVAKGKEIYIN
                     RINIFGNTRTRDKVVRRELRLAEGDRYSSTGLKRSKERLTNTQYFKEIDLKLVKTEDP
                     EKINLDVAVQEKPTGTFNVSIGYSTSEKAIIGGSISQQNLLGTGRKLYFNAGIGDITN
                     SFNASIIDPYIFDLNLSAGLGVSHYTREMDSYDYRIAGGNLSFVRPMTEFTKLNTRYK
                     YERTKIYNIDIDASHIIKDQAGTSSTSSVTAGLNRNTIDNIMNPSKGSNSEISFELAG
                     GPFGAENDFYKSIAQYGKYIPAGFWDSTFFLKGTAGMIRGYNGKKIPIYEKFYVGGLH
                     TVRGFKYGEAGPMDIYGDAVGGKNQLFFNLEWIFPIYVPVGLKGVIFFDAGHGFNKNK
                     GFLLNDARTSAGTGIRWFSPFGPIRIEVGFNLNPKKDEKRSVFDFAIGSQF"
     gene            1694..2218
                     /locus_tag="PK125_09620"
     CDS             1694..2218
                     /locus_tag="PK125_09620"
                     /inference="COORDINATES: protein motif:HMM:NF015871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HPB38400.1"
                     /translation="MKNVLLFTGIIALLVIPWTAYGQVSIAYVDLQKIMVESNKGKEA
                     KQTLTQEADRLKKTLDSKQDELQKMKDAIEKQGTTITPEARTEKEKQYQNKLKDYQRL
                     ANDYQTELQQKDMELTQNMLKELEGVIKSLGEKEKYTIIVEKSQVLYASPAIDVTSKV
                     INLFNEATKKKPTK"
     gene            2218..3252
                     /gene="lpxD"
                     /locus_tag="PK125_09625"
     CDS             2218..3252
                     /gene="lpxD"
                     /locus_tag="PK125_09625"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: protein motif:HMM:NF002060.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HPB38401.1"
                     /translation="MIRLKKLAEEVNGHLVGEGNTPITGVAGIKEAKKGEISFLTNVS
                     YQKYLKDTKASAVIVGENTNVQDIGNLNAIIVKNPPLAYAIVADLFNTRTHKQTVGRS
                     RLAFIAKGAKISKTASIAPFVHIEDGVVIKKNVTIFPFVYIGRNSTVGDGTQIFPNVT
                     IYEGVKIGRNVIVHSGTVIGSDGFAYTWDGSKHVKIPQLGSVEIEDEVEIGSNVTIDR
                     ASLEKTIIKKGVKIDNLVQIAHNVTIGENSIIVAQVGIAGSATIGKNVVLAGQAGVRD
                     HVIVGDNVQAGGQTGITKDVPANSLIMGTPHLPFKEWAKLQGYFKMLPQLFAKMKQVE
                     KKLNLEGEDD"
     gene            3245..3673
                     /gene="fabZ"
                     /locus_tag="PK125_09630"
     CDS             3245..3673
                     /gene="fabZ"
                     /locus_tag="PK125_09630"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: protein motif:HMM:NF000582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HPB38402.1"
                     /translation="MIDINEIMTLMPHTYPFLLVDRVVEFEPAKHAVGIKNVTFNEPF
                     FTGHFPGMPIMPGVLIIEAMAQTGGVLAFKSFPGMKGSVLFIGIDNARFRKPVIPGDQ
                     LKLVVEVVKHKREIWVFEGKAYVNDEMVAEARIMAMLKQD"
     gene            3682..4449
                     /gene="lpxA"
                     /locus_tag="PK125_09635"
     CDS             3682..4449
                     /gene="lpxA"
                     /locus_tag="PK125_09635"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: protein motif:HMM:NF003657.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HPB38403.1"
                     /translation="MIHPTAIVDRKAEIDSSVEIGPYCIIEGNVIIKKESKLLSHIVV
                     QGRTEIGENCTISPFAALGGPPQDISYKKEDTGLCVGNNNIIKEYVTMNRGTIHGKGI
                     TRVGDNNFIMAYSHIAHDCIVGNNIIMANCATLAGHVEVSDSVIFAGLCAVHQFCKIG
                     KHAFISGLTGVPKDVPPFMIAAGSRAKLYGLNVVGLERHGFSKEEISKLKKAYRILFR
                     SALPLTESLKIIKEELSGDHIRELVEFIGSSKRGICR"
     gene            4440..5363
                     /locus_tag="PK125_09640"
     CDS             4440..5363
                     /locus_tag="PK125_09640"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HPB38404.1"
                     /translation="MPIKMALVGCGHMGKIHLQKLSSFDEIQIVGIVDVDTKRANDLA
                     QPINVPAFNNYKKLLGNVDGVIIATPTETHYQIAKDFLKSRTHILLEKPITSRQDQAQ
                     ELIDLAQARQLILQVGFLERFNPAFSKILPIIKKPLLIESRRSSEFTGRSTDIDVVLD
                     LMIHDIDLILSIVREDVCDIRAQGVSFITDKLDMASARIEFSNGCIADLNASRISSKK
                     ERTLTVFEGNQIFFIDLLNRRVVYSAKEQNKDIDTEEYTVDPIDAVKYEISLFIQSIL
                     TGTAPIVRGEDGLRALTLADQVKQRIAENRL"
     gene            5320..6528
                     /gene="lpxB"
                     /locus_tag="PK125_09645"
     CDS             5320..6528
                     /gene="lpxB"
                     /locus_tag="PK125_09645"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: protein motif:HMM:TIGR00215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HPB38405.1"
                     /translation="MLIRSSSALQKIDCNNNAPIKAVIITGELSGEIHASHLVDALNS
                     SMPFEFSGMGSTRLQEVGVRIIYDYSNISVTGISEIFGKLKYIREAYNIMKRHLIKEK
                     PALLIIVDFPGFNLKIAQFAKKHGVPVIYFIPPQVWAWRRSRIRKIRDRVNRVLCILP
                     FEKKLYEEYDVDVAYVGHPFFKTVRPVFLKSEFYQKLGVEPQGPVITILPGSRVNEVH
                     KHMPILLAIIEKLKEQVHQPTVLLPLAENIDPGLVQKYVTAPNSIKIIKGLSHDAIAH
                     SDLALAASGSVTLEAAILGTPTVVIYRISVLSYLLAKILVDVKYISLPNIIANKEIFP
                     EFIQQLDPEKIAEKALYMLKNGREGIKDDIKTIRDQFSNFDSYNRARDEIVLFLRHIY
                     GSLPKTSSVC"
     gene            6494..8257
                     /gene="msbA"
                     /locus_tag="PK125_09650"
     CDS             6494..8257
                     /gene="msbA"
                     /locus_tag="PK125_09650"
                     /inference="COORDINATES: protein motif:HMM:TIGR02203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005319 - lipid transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006869 - lipid transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A export permease/ATP-binding protein
                     MsbA"
                     /protein_id="HPB38406.1"
                     /translation="MVAYLRLLQFVKPYRFKLALAMIFMSLVAATNGLTAYIVKPVLD
                     KIFFEKNAEMLYIIPFAVILLYLCKGIFEYLQAYLMGFVGQKVVNDIRNLVFDSLQRQ
                     PLSFFDKVPTGANISRVINDVNQVQTAITDSFTAVMKDAFSIFALIFVVFYRDWKLAT
                     VALVILPFSAYPIVSFGKKLRKISVNTQRTVARLTSFLHETITGQRIVKAFSMEEYEN
                     QRFKVENDNLFKINLKRYKIRALSHPVMEVLGGIAIGVVIWYGGRQIIAGHSTPGNFF
                     SFTAALLMLYEPIKRLNKENHSIQQGLGASQRVFELIDREPEIIEKKDAVHLHRVQGI
                     IEFKDVFFKYEDKMILKNVNLKINRNEVIAIVGQSGVGKTTLVNLIPRFYDIASGLIK
                     IDDVDIRDVTLNSLRDNIALVTQDVILFNDTIKNNIAYGVEVDMAKIEEAARTAYAHE
                     FITKLSKSYDTVVGEKGARLSGGQKQRIAIARALHKNAPILILDEATSSLDTVSELEV
                     QKALENLMKGRTTIIIAHRLSTVMNADRIIVIDGGTIVQEGAHAQLIQADGPYKKLYD
                     LQFRDNENKKIIKLGKRAKNE"
     gene            8250..8783
                     /locus_tag="PK125_09655"
     CDS             8250..8783
                     /locus_tag="PK125_09655"
                     /inference="COORDINATES: protein motif:HMM:NF015961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HPB38407.1"
                     /translation="MNKQIKTFFMVKILPPLIFLFLCMLRTTLKIKQVNNGRVIEGWK
                     KGENFIFCFWHGRLLMMPFANVRGKGKVLISRHRDGELIARVMAFFRLGSIRGSFRKG
                     TVSSIREIINSLREGYDVAITPDGPKGPRHTVKDGIVELSRLTGKALVPITYSASKKK
                     LFTLGTGLSFLIHFQQC"
     gene            8902..10182
                     /locus_tag="PK125_09660"
     CDS             8902..10182
                     /locus_tag="PK125_09660"
                     /EC_number="2.4.99.12"
                     /EC_number="2.4.99.13"
                     /inference="COORDINATES: protein motif:HMM:NF016311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-D-manno-octulosonic acid transferase"
                     /protein_id="HPB38408.1"
                     /translation="MWKIIYNLLAHCALPVFVIYGLTQKKIRRNLFERLLATTKKSYV
                     EQAMWIHAASVGEAAIAENLVAYLRQYTDLQRFIITTNTYYTKDMLRARLGHDIPVFA
                     LPLDLSYLINRFIATSDFKALVIIETEIWPNLIWQVRKRHIPVIIVNGRISDRTIATY
                     RRFSSFLAHVLNHVEHVAAQSSIHKDRFVSIGMNPQKITITGNIKYYRMLKKNNTITP
                     KENMITFGSIKEKELEIILPVIISLKNDLQDILIYIAPREIHLSAIVEDRLSKSFNVA
                     RLSAIKKRVKEEVDIVVVDTMGDLMGIYEKSKLAFVGGSLAPYGGQNILEPLFFATPV
                     IFGPHMENFKDIADMVLSHKAGIMVQSGDDLYTGIKSILSDDSIQKEMGNAGKTIIQQ
                     QQNVMKRTVDVILKAIDMSDGRRENRGGLDQREK"
     gene            10280..11035
                     /gene="mazG"
                     /locus_tag="PK125_09665"
     CDS             10280..11035
                     /gene="mazG"
                     /locus_tag="PK125_09665"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF007113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HPB38409.1"
                     /translation="MEEFSKLVELMATLRSDKGCLWDKKQTIDSFKTFVLEEVYELID
                     SIEKANYSNIKEELGDLLFHIVFISQICKEGGFFDITDVVKDVYNKMYNRHPHVFGDM
                     PRDTPIEVKWEELKKNEKGDYSPLQNIPHILPALLRAYIISKRAAKVGFDWPGLEDVH
                     EKMHEEIVELQKAEQSGNIESIKEEIGDLLFTIVNISRFHNIDPEDALRSTSNKFVNR
                     FSYIEKNTDLSSATLEIMDRLWNEVKGMEKKGH"
     gene            complement(11045..11248)
                     /locus_tag="PK125_09670"
     CDS             complement(11045..11248)
                     /locus_tag="PK125_09670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013859272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HPB38410.1"
                     /translation="MPVGKVKWFNDSKGYGFIEQESGEDVFVHFSAIKQNGFKSLKTG
                     EKVEFEIVKGPKGPQASNVVKSE"
BASE COUNT         3619 a         2261 c         2453 g         3020 t
ORIGIN      
        1 atagtaagct caaaagtaca atgatggtcc gtgaaaaagg atttttttct tggtttaccg
       61 ggtcgggcat acttgaagag gatgcccttg acgatgacag gaagagactt gaatcatttt
      121 attctgacaa tggatatata aaggccaaga taggagtgcc cgacatttca atttcaaagg
      181 atggcaggac catcagtgtt gttatcccaa ttgaagaagg tgctatctat aggattggaa
      241 aaattgattt tgctggtgat cttatatttc ctgttgacgt tctgaataaa gacctcaaga
      301 gcaaaccagg caacattttc agatcttcac agtatcgtga ggatgtgatc atgcttacag
      361 acctctacca ggaccgcggg tatgctttca ctgatatctc ccctgttact ctggttgatg
      421 actccactca aaagatagac cttacgttca atgtagcaaa aggaaaagaa atttacatca
      481 acaggataaa tatatttggc aacacaagga cgcgggacaa ggtggttcga cgggagttga
      541 gactggcaga gggtgaccgg tactctagta cgggattaaa gagaagtaaa gagagactca
      601 caaacaccca gtactttaag gaaattgatc ttaaacttgt caaaacagaa gaccccgaaa
      661 agataaacct tgatgtggct gttcaggaaa aaccaactgg cacatttaat gtctctattg
      721 ggtacagcac atcagagaag gcaattattg gcggatctat ttcacagcaa aatttgctcg
      781 gaacaggaag aaagctctat ttcaatgcag gcataggaga cataacgaat agttttaacg
      841 ccagcattat tgacccctat atcttcgatt tgaacctttc tgccggactt ggcgtttctc
      901 attatactcg ggaaatggat agctatgact atcgtatagc cggtggaaat cttagttttg
      961 taagacccat gactgaattc actaaattga acacaaggta caaatacgag agaacgaaaa
     1021 tatataatat tgacattgat gcaagtcata tcatcaagga tcaagcaggg acatcgagta
     1081 caagcagcgt tactgccggg ttgaacagaa acaccataga taatattatg aatccctcca
     1141 agggttcaaa ttcagagatt tcatttgaac tggcaggcgg accttttggt gcagagaatg
     1201 acttctacaa aagtattgca caatatggga aatatattcc agccggattc tgggactcta
     1261 cttttttcct gaaggggact gccggtatga tacgcgggta caatggcaaa aaaattccta
     1321 tatatgaaaa attttatgta gggggtttgc atactgtcag aggcttcaag tatggtgaag
     1381 ctggtccaat ggacatatat ggggacgctg tgggaggaaa gaatcagttg ttcttcaacc
     1441 ttgagtggat atttcccatc tatgtacctg tggggctcaa aggtgtcatc ttttttgacg
     1501 ctgggcacgg tttcaataaa aacaaaggct ttcttttgaa tgacgcacgg acctcagcag
     1561 gaacaggcat ccgttggttt tctcctttcg gaccaatacg gatagaagtg ggattcaacc
     1621 tgaatcctaa gaaggacgaa aaaagaagtg tatttgattt tgcaattggt tcacaatttt
     1681 aataggaggc agcatgaaaa acgttttatt attcacagga ataatcgctc tattagtaat
     1741 tccatggacc gcgtacggtc aggtcagtat tgcctatgtt gatcttcaga aaataatggt
     1801 cgagtctaat aagggtaaag aggcaaaaca gactttaacc caggaggcag acagactgaa
     1861 aaaaacgctt gatagcaagc aggatgagct gcaaaaaatg aaagatgcta tagagaaaca
     1921 aggcaccaca atcacgccgg aagctaggac tgaaaaagaa aaacaatatc agaacaaact
     1981 gaaggattat caaaggctgg caaatgatta tcagaccgaa ctgcaacaaa aggacatgga
     2041 attgactcag aatatgttga aggaacttga gggggtcata aagagtctcg gcgaaaaaga
     2101 aaaatacacc attatagttg aaaaaagtca ggtgctgtat gcttcaccgg ctatcgatgt
     2161 gacttcaaaa gtaataaacc tttttaatga ggcgacaaag aaaaaaccaa ccaagtgatg
     2221 ataaggttaa aaaaattagc tgaggaagtt aatggtcact tagttggaga aggaaataca
     2281 ccgataaccg gtgttgcagg aatcaaggaa gcaaagaagg gagagatatc ttttctcacc
     2341 aatgtaagtt atcagaaata cctgaaagat acgaaagcct cggccgtaat agtcggagaa
     2401 aataccaatg ttcaggatat tggcaatctg aacgccatta tcgttaaaaa cccgccactc
     2461 gcgtatgcta tagttgccga cctgtttaac accagaactc ataaacaaac tgttggacga
     2521 agtagactag cctttatagc aaaaggggct aaaatttcaa aaacagcatc tattgcacct
     2581 tttgtacata tagaggatgg cgtagtcatt aaaaaaaatg taacaatctt cccatttgtt
     2641 tatattggca gaaactcaac agttggggac ggaacacaaa tctttcccaa tgttaccatc
     2701 tacgagggtg taaaaatagg taggaatgtg attgtccata gtggtacagt cattggtagc
     2761 gatggattcg cctatacatg ggatggaagc aaacatgtaa agattccgca acttggctcc
     2821 gttgaaattg aagatgaagt tgagatagga tccaatgtta caattgacag ggcatcgctg
     2881 gaaaaaacaa taataaaaaa gggtgtgaaa atcgacaacc ttgtgcaaat agctcataac
     2941 gttactattg gtgaaaactc aattattgtt gcccaggtag gcatagcagg cagcgccacc
     3001 attggtaaaa atgttgtcct tgcaggacag gcaggagtca gagaccatgt gatcgtggga
     3061 gacaatgtgc aggcaggcgg acagacagga ataaccaaag atgttccagc aaattctctc
     3121 attatgggaa cacctcacct accctttaaa gaatgggcta aactgcaagg ctatttcaag
     3181 atgttgcccc agctttttgc aaaaatgaaa caagtagaaa aaaagcttaa cctggagggg
     3241 gaggatgatt gatattaatg aaataatgac tctcatgccg cacacatacc cttttcttct
     3301 cgttgataga gttgtggaat ttgaacctgc taaacatgcg gtgggcatca aaaatgttac
     3361 ttttaatgag ccattcttta caggacattt tccaggcatg cctattatgc ccggtgtttt
     3421 gattatagaa gcaatggctc aaaccggggg agtcttggca ttcaaatctt ttcctggtat
     3481 gaaagggtct gttctgttta ttggcattga caacgcgaga ttcagaaaac cggttatccc
     3541 aggtgaccag ttgaaactgg tggttgaagt agtaaaacac aaaagggaga tatgggtttt
     3601 tgagggaaaa gcatacgtca atgatgagat ggttgcggaa gccagaatca tggcaatgtt
     3661 aaaacaggat taggagggtc tttgatacac ccaaccgcta ttgtggacag gaaggctgaa
     3721 atcgattcat cggttgaaat aggaccttac tgcatcattg aaggaaatgt aattataaaa
     3781 aaagaatcca agctgctttc tcacatagta gttcagggga gaacagagat tggggaaaac
     3841 tgtacgatta gcccctttgc agcccttgga gggcctccac aggatatttc ttataaaaaa
     3901 gaggatacag gcctctgcgt gggcaacaac aacatcataa aagaatacgt gactatgaac
     3961 agggggacca tacacggcaa gggcatcaca agggtaggag acaataactt tatcatggct
     4021 tattcccata tcgctcatga ctgtattgtg ggcaacaata ttataatggc aaattgcgcc
     4081 acccttgcag gccatgtcga ggtttcggat tctgttattt ttgcgggtct ttgtgcagtc
     4141 caccagttct gcaagatagg taaacatgcc ttcatcagcg gccttacagg tgtacccaag
     4201 gatgttcctc ccttcatgat cgctgcagga agcagggcta aactgtatgg tctgaacgtt
     4261 gtaggattgg aaagacacgg attttcaaaa gaagaaattt caaagctgaa aaaggcctat
     4321 agaatcctgt ttaggtcggc cctgcctctg acggaatcgt taaagatcat caaagaagaa
     4381 ctgtctggtg atcatattag agaacttgtt gagttcattg ggtcatccaa gaggggtata
     4441 tgccgataaa aatggccctt gtaggttgtg gccacatggg taaaatacac cttcaaaagc
     4501 tatcatcttt tgatgaaatc cagattgtcg gtattgtaga tgttgacaca aagagggcca
     4561 atgacctggc tcaaccaatc aatgtacctg cttttaacaa ttataaaaaa cttctgggaa
     4621 atgtggatgg tgtcatcatt gcaaccccca ctgaaactca ttaccagatt gccaaggact
     4681 tcctgaagtc caggactcac atattattag aaaaaccaat aacttcacgt caagatcaag
     4741 cccaggaact tattgatctc gctcaggcca gacaactcat tttacaggta gggttcctgg
     4801 agcggtttaa tcctgcattc tcaaaaattc tccccatcat taaaaagccc cttcttatag
     4861 aatcacgcag gtcaagtgag tttacaggga gatcaacaga tattgacgtt gttctcgacc
     4921 ttatgatcca tgacatagac ttgattctgt ccatagtcag ggaagatgtg tgcgatatac
     4981 gtgcccaagg agtttcgttc attacagata aactggatat ggcaagcgcc agaatagaat
     5041 tcagcaacgg atgcatcgct gacctcaatg ccagccgcat ctcatcaaaa aaagagagaa
     5101 cgcttacagt ctttgagggg aatcagattt tttttattga tcttctaaac cgacgggttg
     5161 tctattctgc aaaagaacag aataaagaca tagatacaga ggaatacacg gttgatccaa
     5221 tcgatgcagt aaaatatgaa atatcacttt ttattcaatc catcctgaca ggcaccgctc
     5281 ctatcgtaag aggcgaagat ggtttaagag ctcttaccct tgctgatcag gtcaagcagc
     5341 gcattgcaga aaatagactg taacaataat gctccaatta aggcagttat cataactggg
     5401 gagctttccg gagaaattca cgcatctcat cttgtggatg ctctgaacag ctcaatgccc
     5461 ttcgagttca gcggtatggg cagtacaaga cttcaggaag tcggagtgcg tatcatctat
     5521 gattacagca acatttctgt tacaggcatc agcgaaatct tcggaaaact gaaatatatc
     5581 cgggaagcgt ataacattat gaagcgccac ttgatcaagg aaaaaccagc tctcctgatt
     5641 atcgtagact ttccaggttt taacctgaaa attgctcagt ttgcaaaaaa acatggagtg
     5701 cctgttatct attttatccc cccacaagta tgggcatgga gaagatcgag gattcgaaaa
     5761 atccgagacc gtgttaacag agtactttgc atcctgccct ttgaaaaaaa actgtacgaa
     5821 gaatacgatg tggatgttgc ttatgtaggg catccatttt tcaaaactgt cagaccggtt
     5881 tttttaaaat cagaatttta ccaaaaatta ggcgtggaac cgcagggtcc tgtgattacc
     5941 atattgcctg gaagcagagt aaacgaggtg cataagcata tgcccatact gcttgccatt
     6001 attgagaagc tgaaggaaca ggttcaccag ccaaccgtcc tacttcctct cgctgaaaac
     6061 attgaccccg gtttagttca aaaatatgtg acagcgccga acagcatcaa aattattaaa
     6121 ggattgtctc atgatgccat tgctcactct gatctcgctt tagcagcctc cggcagtgtc
     6181 acccttgagg ctgcaatcct tggtacccct actgttgtaa tctacaggat ttccgtctta
     6241 tcctacctcc tcgccaaaat actcgtggat gtaaaatata tcagcctgcc caatatcatt
     6301 gccaacaaag aaatttttcc agaattcatt cagcaactag accctgaaaa gattgcagaa
     6361 aaagcgctat atatgttaaa aaatgggaga gagggtataa aggatgacat caaaacgata
     6421 cgtgatcaat tcagtaactt tgactcgtac aaccgggcca gagatgagat tgtcctgttt
     6481 ttaaggcata tttatggtag cctacctaag acttcttcag tttgttaaac cataccggtt
     6541 taagctcgcc ttagccatga ttttcatgtc tctagtcgct gccaccaatg ggctaacagc
     6601 ctatattgtg aaacctgtcc ttgacaaaat cttttttgaa aaaaacgccg aaatgcttta
     6661 tatcataccc ttcgcggtca tcctccttta cctatgcaaa ggaattttcg aatatctcca
     6721 ggcatacctc atgggttttg ttggtcaaaa agttgttaat gacattagaa atcttgtttt
     6781 tgattcactc cagcgtcagc ccctttcttt cttcgacaag gtgccaactg gcgcgaacat
     6841 atccagggtt atcaacgatg tgaaccaagt ccagaccgcc ataacagatt ctttcacagc
     6901 tgtcatgaaa gatgcctttt ccatttttgc ccttattttt gtggtcttct acagagattg
     6961 gaagcttgca actgttgctc tagttatatt gccattctcg gcatacccaa tcgtttcttt
     7021 tggaaaaaaa ttaagaaaaa taagtgtgaa tacccagaga actgttgctc ggttaacgag
     7081 ctttcttcac gaaacaatta cagggcagcg aatcgttaag gctttttcca tggaggagta
     7141 tgagaaccag cggttcaaag ttgaaaatga caaccttttt aagatcaatc tcaagcggta
     7201 caagattaga gccctgtcac atccggttat ggaagttctt ggcggtattg cgattggtgt
     7261 tgtcatctgg tacggtggcc gacaaattat tgccggtcat tctacaccgg gaaatttctt
     7321 ttccttcact gcagcgcttc ttatgctcta cgagcctatc aagagattaa ataaggagaa
     7381 tcacagcatt cagcagggac tgggtgcaag ccagcgagta tttgagctta tcgacagaga
     7441 gcctgaaatt attgaaaaga aggatgctgt tcaccttcac cgggttcagg gtatcataga
     7501 attcaaggat gttttcttca aatatgaaga caagatgatt ttaaaaaatg ttaacctaaa
     7561 aattaacagg aatgaggtca tcgctatagt cgggcagagc ggggtgggca aaacaactct
     7621 tgtcaacctg attccccgat tttacgacat tgccagcggg ttaattaaaa ttgacgacgt
     7681 tgacatccgt gatgtaacac tcaactctct tagagataac atagccctgg ttactcagga
     7741 cgtcattctt tttaatgata caatcaaaaa caatatcgcc tacggggttg aggttgatat
     7801 ggctaaaatc gaagaggctg cacggactgc atatgctcac gaattcatca caaaactctc
     7861 aaagagctat gataccgttg ttggtgaaaa aggtgccagg ctatcaggag gacaaaagca
     7921 gaggattgcc atagcaaggg ctcttcacaa aaatgctcct atcttgatcc tggatgaggc
     7981 aacaagttcc cttgatacag tatccgaatt ggaagtgcaa aaagccctcg aaaatttgat
     8041 gaaaggaaga accaccataa ttatcgctca tcgcctgtca acggtgatga acgctgacag
     8101 gatcattgtt attgatggtg gaaccattgt ccaggaaggt gcacatgccc agcttattca
     8161 agctgacggt ccctacaaga aactttatga tctccagttt cgtgacaatg aaaataagaa
     8221 aatcataaaa ctcggaaaac gagccaagaa tgaataaaca aattaaaact ttctttatgg
     8281 ttaaaattct gccaccgctc atatttcttt ttctctgtat gttgcgaacc accctaaaga
     8341 ttaaacaggt aaacaatggc cgcgtgattg agggatggaa aaaaggagag aacttcatct
     8401 tctgtttctg gcatggaaga ttactaatga tgccttttgc aaatgtgaga ggaaaaggga
     8461 aggttctcat cagcaggcat cgcgacgggg agttgattgc tagagttatg gcatttttca
     8521 ggcttggctc catccgcggc tcttttcgca aggggactgt atcatccata agggaaataa
     8581 ttaacagctt aagagaaggt tatgatgttg ccataacacc tgacggaccc aaaggaccgc
     8641 ggcatacggt aaaagacggt attgttgaac tatcacggtt gacaggaaag gctctcgttc
     8701 ccataaccta tagcgcaagt aaaaaaaaac ttttcactct tgggacaggt ttatccttcc
     8761 ttatccattt tcaacagtgc tgatactctg gggcaacccg attcatattg attcaaaagc
     8821 cacgcgagaa accttagaac ttaaaaaaca tgagcttgaa gttacactca ataacctcac
     8881 cgaacaggct gacttactgg catgtggaaa ataatctaca atcttttagc ccactgtgcc
     8941 ttgccggttt tcgtcatcta cggtttaacc cagaaaaaaa tccgaagaaa tctttttgaa
     9001 agacttcttg caacaacaaa aaaatcatat gtcgagcagg caatgtggat acacgccgca
     9061 tcggtgggag aagcagccat tgcagaaaac ctggtagctt atctgagaca atacactgat
     9121 cttcaacgct ttataatcac aaccaacacg tattatacaa aagatatgct tagagcaagg
     9181 cttggtcacg atatcccagt ctttgcattg cctcttgatc tgagttacct tatcaaccga
     9241 tttatcgcaa cctctgattt caaggcctta gttattattg aaaccgagat atggcccaac
     9301 cttatttggc aggtcagaaa acgacatatc cctgtaatta ttgtaaacgg aaggatttct
     9361 gatcgaacca tagctacata tcgccggttt tcttcatttc tggcgcacgt tctcaatcat
     9421 gttgaacatg ttgctgccca gtcgtcaatc cacaaagatc gcttcgtatc catcgggatg
     9481 aatcctcaaa aaataacaat cactggaaac attaagtact acaggatgct aaaaaaaaat
     9541 aacaccataa ctccgaaaga gaatatgatc acctttggca gtattaaaga gaaagaactc
     9601 gaaataatcc ttcctgtcat tatctctttg aagaacgatt tgcaggacat tcttatctat
     9661 attgctccac gggagataca cctctcagcc atagtcgagg ataggctctc gaaatcattt
     9721 aatgttgcaa gactctcagc aatcaaaaaa agggtgaaag aagaagttga tattgtggtg
     9781 gttgatacca tgggtgatct tatgggcatc tatgaaaaaa gcaagcttgc cttcgtaggc
     9841 ggaagtctgg ccccttacgg aggccaaaat atacttgagc cattattctt tgccacgcca
     9901 gttattttcg gaccccacat ggaaaatttc aaagatattg cagatatggt cctgagccat
     9961 aaagcaggca taatggtcca gagcggcgac gatctctaca cagggataaa aagtattcta
    10021 tcagatgatt ctatacaaaa agagatggga aatgcaggca aaacgatcat tcaacaacaa
    10081 caaaatgtga tgaagagaac agtggatgta atattaaaag cgatagatat gtctgatggc
    10141 aggcgggaaa atcggggcgg gctggaccaa cgggaaaaat aagtcaagaa ctcatagact
    10201 atagccggca actcatgggc aggcaagggt tcaaagctgc tgctctcagc ttgcagctat
    10261 tagctaaatc ggaggtttta tggaggagtt ttcaaaactt gttgaactca tggccacact
    10321 caggagtgac aaaggctgtt tgtgggataa aaaacaaacc attgactcat tcaagacatt
    10381 tgttcttgaa gaggtttacg aactgatcga ttctattgaa aaagccaatt attcgaacat
    10441 aaaggaagaa cttggggatt tgctcttcca catagttttt atctcccaaa tctgcaagga
    10501 aggaggattt tttgatatca ctgatgtagt aaaagatgtg tataacaaaa tgtataaccg
    10561 ccatccccat gtgttcggag acatgccccg tgacacaccc attgaagtaa aatgggaaga
    10621 gttaaagaag aatgagaaag gcgattattc ccctctccag aatatacccc acattcttcc
    10681 tgcgcttctc agagcctata tcatctccaa aagagctgca aaagttggtt ttgattggcc
    10741 cggactggaa gatgttcatg aaaagatgca cgaagagatt gttgagctcc aaaaggctga
    10801 acagtcagga aacatcgagt caatcaagga agagattggg gaccttttat tcaccatcgt
    10861 taatatctct cgctttcata atattgatcc tgaagatgcg cttcggtcaa catcaaacaa
    10921 gtttgtgaat aggttcagtt atattgaaaa aaatacagac ctttccagcg ctaccttaga
    10981 gataatggac agattgtgga atgaagtaaa aggtatggaa aaaaaggggc attagcccct
    11041 ttttttattc gctttttact acattggatg cttgcgggcc tttcggacct ttgacaattt
    11101 cgaattctac tttctccccc gtttttaacg acttaaaacc attctgtttt attgcagaaa
    11161 aatgtacaaa aacgtcttct ccactctctt gctcaataaa accgtacccc ttagaatcgt
    11221 taaaccattt taccttacct actggcatac tgctaaactc ctttcagtga aataattaag
    11281 cattcctgct tgttgagaat catattgtta tttatcggca gctgtcaagg aaaatcttaa
    11341 aaaattttaa taa
//
LOCUS       DAOOMC010000090        11277 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdus sp. isolate AMR_MDS_2685
            NODE_6478_length_11277_cov_6.631616, whole genome shotgun sequence.
ACCESSION   DAOOMC010000090 DAOOMC010000000
VERSION     DAOOMC010000090.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864760
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdus sp. (wastewater metagenome)
  ORGANISM  Syntrophorhabdus sp.
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae; Syntrophorhabdus.
REFERENCE   1  (bases 1 to 11277)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11277)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,729
            Genes (coding)                    :: 2,708
            CDSs (with protein)               :: 2,708
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 7 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11277
                     /organism="Syntrophorhabdus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6478_length_11277_cov_6.631616"
                     /isolate="AMR_MDS_2685"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2699756"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(599..1918)
                     /locus_tag="PK125_09675"
     CDS             complement(599..1918)
                     /locus_tag="PK125_09675"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF013015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HPB38411.1"
                     /translation="MLTSQPLSLYSFPRAIVHIDGDAFFASCEQARNPSYKGKPLITG
                     KERGIASSLSYEAKAMGVKRGMRLSEIKKLCPQIIILPSDYETYCLLSKRLFEIVRRY
                     TNEVEEYSIDECFADLTGLRRPLRMNYTMMAERIQKDLAIELGFTFSVGLGPNKIIAK
                     IGSKWKKPAGLTVIPAREIHLYLEKLPVEKVWGIGPQTTAYLNKNKVFTALDYARKNE
                     EWIKKRLTRPFLEIWKELNGHFVLPLETKEKEDYASIQKVKTFTPPSRDPVYVFSQLS
                     KNVENACMKARRYKLEAMRLYFFLKTQDFNYRGAEIKLSRSSAFPNEIIRIIRPLFHE
                     VFDPALEYRATGVCLFKLKPQSSLQLDLFGEVLAVEKFRKIFESVDVIRNKYGKHTVC
                     LGSSLLANKSSQHLNERGDLSERSKFLLPGETKRKRLGIPMFLGEVI"
     gene            complement(1921..2484)
                     /gene="lexA"
                     /locus_tag="PK125_09680"
     CDS             complement(1921..2484)
                     /gene="lexA"
                     /locus_tag="PK125_09680"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: protein motif:HMM:TIGR00498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HPB38412.1"
                     /translation="MEKHSRAIVDFYRNTKRMPSYQEIMKLTGFKSKNAVFKLINKLV
                     SAGLIEKDSTGKIAPKRIFGEIKILGVVEAGFPSPAEEELLDTMSLDEYLISNKEATF
                     ILQVSGNSMIDAGIMPKDLVLVERTNEAKPGDIVIAEVDGKWTMKYLRQKGNQMYLEP
                     ANKKYTPIYPKEELKISAVVKAVIRKY"
     gene            2513..3232
                     /locus_tag="PK125_09685"
     CDS             2513..3232
                     /locus_tag="PK125_09685"
                     /inference="COORDINATES: protein motif:HMM:NF014626.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0018142 - protein-DNA covalent
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HPB38413.1"
                     /translation="MSRIFSGCRNIQKAVYSLDMCGRFSNFSPLDVLRNRFRVDPPDE
                     PVYPRYNAAPGQKLIVIPMDDPHKMHFYKWGLIPSWARDPKIGNSLINARSETLKEKP
                     AFRGSLNRRRCLVLADGFYEWKTTSGRKVPYRVELRGREPFAMAGLCSTWKDPDGKSI
                     DTFAIVTTNANEIVGTIHDRMPVIFTPEQESKWIDLSFDFQEVNQYLLPYSENDMEIY
                     PISTLVNNPKNDIPEILEPIR"
     gene            3463..4605
                     /locus_tag="PK125_09690"
     CDS             3463..4605
                     /locus_tag="PK125_09690"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HPB38414.1"
                     /translation="MDLYISSDLTDIQKAVSEFCEKEFSQEYLQRYEDEHNYPRELYE
                     KIGTLGFIGVGFSEDIGGAGYGALGHVVAVTEMCRKLPGMGMAMSLGYIPSFVVDISG
                     TKEQREKYIRPLIEGRYVAAFSVTEPDAGSDISVLKTRMEEKNDCFVVNGSKIFTTNA
                     GYADFYTLLAMDGNSFTALLFDKTVIDDNGPRRFEINDLGRKMGINTTMSGELVLSDF
                     HVPKECVLGERGKGKKTALAAFELSRVVIAGQAFGGMLYGYERAVDYAKKRKQFGNSI
                     IKFQSIEHDLVDMYVEIEKCRNLLYKTAWLYDRKDPESNKYASMVKLTIPENAISVLN
                     RCIDVFGGYGYMRDQGLEGALRDIRITSIYEGTNKIQKNVIARMLY"
     gene            complement(4657..5301)
                     /locus_tag="PK125_09695"
     CDS             complement(4657..5301)
                     /locus_tag="PK125_09695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB38415.1"
                     /translation="MTVQYFKVFLILIVAAGIWACSVPLVVTVDIPDSRIPLNTGVRD
                     ASFLTNMEKEVIVELNAIRTNPRQYAEFLKSLRGSPQWSEGLEETILFVEKKEPLPAF
                     RTSKGLSLAARNLVSDRGPEGLTGHRDKDGKSTLERMNCCGQPGGKFGEYLGYGYLEG
                     AALVAKMAIDEGAAGKEGEKYIFERDFLVVGVACGPHKSYRTMCVVDFAGSYRE"
     gene            complement(5318..5665)
                     /locus_tag="PK125_09700"
     CDS             complement(5318..5665)
                     /locus_tag="PK125_09700"
                     /inference="COORDINATES: protein motif:HMM:NF015893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HPB38416.1"
                     /translation="MGINIQIFGTLKCQETKKAQRYFKERGIPFQFVNLAEKGLSQGE
                     LNSVKAVFGIDALIDREGKEYKRLNLEYLTHNVEEELMSKPLLFKTPIVRNRGKATVG
                     YAPHVWKEWVDYP"
     gene            5926..6105
                     /locus_tag="PK125_09705"
     CDS             5926..6105
                     /locus_tag="PK125_09705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB38417.1"
                     /translation="MIYSIFKKLQKRKSGDIRGMSGYCSANPKKDGGKNSPESTDIRF
                     LNDLGYTMRETGVMV"
     gene            6102..6374
                     /locus_tag="PK125_09710"
     CDS             6102..6374
                     /locus_tag="PK125_09710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB38418.1"
                     /translation="MTGRKPCQEFHLTPRGWISGTRMECGVIQGEIIDRPPEAIETWL
                     EEMVLSYLHCADVYSWRLIWFDPTMTEGDRKKIRSRFEKPSDEFPG"
     gene            6486..7592
                     /locus_tag="PK125_09715"
     CDS             6486..7592
                     /locus_tag="PK125_09715"
                     /inference="COORDINATES: protein motif:HMM:NF012927.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavin oxidoreductase"
                     /protein_id="HPB38419.1"
                     /translation="MSKLFETTTIKNLILPNRFVRSATWEGLAADDGAVTQRLIDVAV
                     RLALGGVGLIISGHAYISREGQAGLWQLGVYSDELIPGLTEMTKAVHQRGGKIAMQLA
                     HAGSKAASHLSGLDPIGPSTAEIGGSHIGREMNKKDIEKVIQAFAAAALRAKRAGFDA
                     IQIHGAHGYLVSQFLSPYFNRRTDEYGGSIQNRTRFVVELLEAVRKAVGSEFPVFIKL
                     NSEDFLPNGLTVDEMISASEILEKAGMDSIEMSGGTFLSGKNYPSRQGKPGQGEHEAY
                     YEAAARRCKKTLKIPLMLVGGLRTFETAERLVLENVADYIAMCRPFIREPELVNRWKS
                     GDRKPALCISDSGCFRPGFEGKGVHCVVEGRLTS"
     gene            complement(7582..7992)
                     /locus_tag="PK125_09720"
     CDS             complement(7582..7992)
                     /locus_tag="PK125_09720"
                     /inference="COORDINATES: protein motif:HMM:NF024773.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HPB38420.1"
                     /translation="MELSDEKKKEILLSAKTIAMVGLSPGNNKPSNRVARYMMEHGYR
                     VIPVNPRYENILGEKSYKSLTDILEKVDVVDIFMQAEHTVPIVKEAIKLKPKVIWLQL
                     DIINDEARAIAEANGIDFVQNLCIKMEHTRLFKT"
     gene            8098..8484
                     /locus_tag="PK125_09725"
     CDS             8098..8484
                     /locus_tag="PK125_09725"
                     /inference="COORDINATES: protein motif:HMM:NF039818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB38421.1"
                     /translation="MMHVRFNRNVMLMCGLLIALGIILSPVLASAQATGKGVKFETYR
                     TAIKKDYGIDIKDFKDQLKGGKADGKDITKYDLKQLLMGIKTEQEHTTNKMIALEITM
                     DHLEKIPDYYDRLAEMEEEALDDLED"
     gene            complement(8528..9115)
                     /locus_tag="PK125_09730"
     CDS             complement(8528..9115)
                     /locus_tag="PK125_09730"
                     /inference="COORDINATES: protein motif:HMM:NF024853.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HPB38422.1"
                     /translation="MKRSPDTICAAIAVTALLLAICIPLRPSFLYAQGVLLPSYGQGK
                     TIVRVYTDYFCGPCRAGEPKVEALLLQLVKTNKIKLMFIDTPAHKTTSLYAQYFLYIL
                     NLKKDFEHALSARRVLFEAASQKITAKEKLEEVLTQKGIGFKPFDPKQTFNAMSQYIK
                     DDGVRATPTIIIDNGTEKQPFVGIDNIVNALELLK"
     gene            9328..9660
                     /locus_tag="PK125_09735"
     CDS             9328..9660
                     /locus_tag="PK125_09735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB38423.1"
                     /translation="MEDQFGRDPQVRYLRRVFAGMEQKQKELLERIGLIPTDYRLKRI
                     REAALKHFEKAWMLAGRRGMVETEDEIAGLYTHCLARVLSGNRISVPTELLPSNEKIN
                     EIIQEVFK"
     gene            9657..9992
                     /locus_tag="PK125_09740"
     CDS             9657..9992
                     /locus_tag="PK125_09740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB38424.1"
                     /translation="MIRLRLFGRCRIYHDPVSPVLRAPAQIGWDAWFRSIDLVTPQPL
                     KGEELLRRTRGWWTVEPTEVAEVVKKNGRLVVGEGGELMVEFEDEQSVQALSTALKEK
                     FGDQVQLAP"
     gene            complement(10075..>10427)
                     /locus_tag="PK125_09745"
     CDS             complement(10075..>10427)
                     /locus_tag="PK125_09745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB38425.1"
                     /translation="GIECLVKIIENKENKFNGEIFNIGNPKNEATVKELAHKLKDIFV
                     NHPSTGHYKKHSEIKEVFSKDFYGDGYQDINFRVPSIRKARALIGWEPKVDLDTALRK
                     TLEYYLDANMKAGC"
     assembly_gap    10428..10437
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(10438..11117)
                     /locus_tag="PK125_09750"
                     /pseudo
     CDS             complement(10438..11117)
                     /locus_tag="PK125_09750"
                     /inference="COORDINATES: protein motif:HMM:NF013530.2"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-4-keto-pentose/UDP-xylose
                     synthase"
BASE COUNT         3037 a         2420 c         2660 g         3150 t
ORIGIN      
        1 tcctccttga ttattgcaga gatgtgctgt gcatacccat catatacatc ctctgtggcc
       61 ctggatagca tagcttcttc caggagtcag ctttatgatg aaaacagcgg gtatgtttta
      121 ttaaaatagt ataacgcctt ttttaccttt ggcaacagaa tttaactgtg gttggggatg
      181 tgcttgaaat gattgggtag atatcatgcc tgtcctccct ttttcagtaa aatctttcag
      241 ggattgcctt tcacatgaga aatctcttaa ccgggcagaa caggcataag gctatcatct
      301 gccggcaaga accatacttt ttggaacttg tctgttacat ccatctcaac cctgtaagag
      361 ttgcagattg tctcaaccat ttccaagctt aaccgctatg ctgacaccag cattgtaccc
      421 ttatggagga aaaggaaggg ttctggcaaa aaacaggagt tgaaacgatt cagttttcta
      481 aaaaatacaa ccagattgta aggaaaaatg agaaaaaatg cgggagttga aatagtagtg
      541 ggtgtacgta atattgtgat ggtttatttc gaatccttac ttttgaagaa ggctgccttt
      601 atatcacttc ccccaaaaac atcggaatgc ccaatctctt cctttttgtc tctccaggaa
      661 gaagaaactt gctccgttcc gataagtctc ctctttcatt cagatgttgt gatgatttat
      721 tggccaataa gctggaacca agacagaccg tgtgcttgcc gtatttattc ctaatcacat
      781 ctacgctttc aaatatcttt ctgaactttt ctactgccaa tacttcaccg aatagatcca
      841 actgaagact gctttgaggt ttgagtttga acaggcagac ccctgtggcc cgatattcaa
      901 gagcaggatc aaatacttcg tggaataatg gccgaatgat cctgattatt tcattaggaa
      961 aggcactgga tctactcagc ttaatctcag caccacgata attgaaatct tgggtcttaa
     1021 ggaaaaaata aagcctcatg gcttccagtt tataacgacg cgctttcatg caggcatttt
     1081 caacattttt ggaaagctga gagaatacat acaccggatc cctcgaagga ggagtaaagg
     1141 ttttaacctt ttggatagat gcataatctt ctttttcttt ggtttccagg ggaaggacaa
     1201 aatgaccgtt cagttccttc cagatttcca gaaagggcct ggtgagtctt tttttaatcc
     1261 attcttcatt ctttctggca tagtcaaggg cagtgaacac cttattctta ttcaaatagg
     1321 ctgtggtctg tggaccaata ccccatactt tttctaccgg aagcttttcc agatacagat
     1381 ggatttctct cgccgggatc actgtgaggc cagccggttt cttccatttc gagccgatct
     1441 tggcaatgat cttattgggc cccagcccta cggaaaaggt gaaacctaat tctatagcca
     1501 gatccttctg gatgcgctct gccatcatgg tatagttcat ccttaatggt cgcctgagac
     1561 cggttaagtc agcgaagcat tcgtcaatgc tatattcttc cacttcattg gtgtagcgtc
     1621 ggacaatctc aaacagcctt ttagaaagga ggcagtaggt ctcgtaatca gaaggaagga
     1681 tgattatctg ggggcagagc ttctttattt ccgagagcct catgccacgc ttcaccccca
     1741 tggccttggc ttcataactt aacgatgaag ctatgccccg ttcttttcca gtgatcaaag
     1801 gtttcccttt ataactcgga ttccttgcct gttcgcagga agcaaagaaa gcatcaccgt
     1861 cgatgtgcac gatagcacgg ggaaaagaat acagagaaag tggttgagag gttaacatgg
     1921 ttaatatttc cggatcactg cttttaccac agcggagatc ttcagctctt ctttgggata
     1981 gattggagta tattttttgt tggcaggctc caggtacatc tgatttccct tctgccgtaa
     2041 gtatttcatc gtccatttac catccacttc agcgatgaca atgtctccgg gttttgcctc
     2101 gttggtcctt tccaccaaca ccagatcttt gggcatgatg ccagcgtcaa tcatggaatt
     2161 gccgctcacc tgtaaaataa atgtggcctc tttattgctg atgagatatt catccaggct
     2221 catggtatcc agaagttctt cttctgccgg tgaagggaag ccagcttcca cgacccctag
     2281 aattttgatt tcgccaaaga tcctttttgg tgctatcttt cctgtgctgt ccttttcaat
     2341 gagccctgca gaaaccagtt tattgataag cttgaatacg gcatttttgg atttaaatcc
     2401 ggtgagcttc atgatctctt gataactagg catgcgcttg gtattcctgt agaagtcaac
     2461 gattgcccga gagtgttttt ccatgccctc agtatagtga acgaaagttc acttgtcaag
     2521 aattttttca ggttgtagaa atatccaaaa agccgtatat tcacttgata tgtgcggacg
     2581 attttccaac ttctcacccc tcgatgtgct ccggaacagg tttagagttg atcctcctga
     2641 tgagccggtc tatccacggt ataatgcagc gcctgggcaa aaacttatag ttattcccat
     2701 ggatgacccc cataagatgc atttttataa atgggggctt attccatcct gggccagaga
     2761 cccaaagata ggcaatagct taattaatgc ccgctcagaa accttgaagg agaaaccagc
     2821 tttccgtgga tcactgaaca ggaggcgatg cttggtattg gcagatggct tttatgaatg
     2881 gaagactact tcgggaagga aggttccata tcgagtagag ttgagaggca gggagccttt
     2941 tgccatggcc gggctctgca gcacatggaa agatccagat ggaaaaagta ttgatacctt
     3001 tgcaatagta accaccaatg ctaatgaaat agttggaact atccatgacc ggatgccggt
     3061 catctttaca ccagaacagg aatcgaaatg gattgatctg tcgtttgatt ttcaggaggt
     3121 aaatcaatac cttctacctt attctgaaaa tgatatggaa atctacccca tctcgacgtt
     3181 ggtcaataac ccgaagaatg atattcctga gatacttgaa cccattcgct gacccataga
     3241 agtcgctcct ggaaaatatc tgtagcatct gaccgaatat gttgctaaac tggttcatgg
     3301 cgctcttcct caaggtggtg tcgagttgat tgtgtctttt tggagctgta cctgaaatcg
     3361 tatattgagc agatgggaga aaagatatcc tgacattgcc aatatgagct atccaccctg
     3421 agtggcctga ttttataata ctttcaagcg agttttttaa ccatggatct gtacattagc
     3481 agtgacctga cagacataca gaaggcagta agcgaattct gcgaaaaaga gtttagccag
     3541 gaatacctcc agcggtacga ggacgagcac aactatccaa gagaactcta tgagaaaatc
     3601 ggcacactgg ggtttatcgg ggttggtttt tctgaagaca taggtggggc aggatatggg
     3661 gcgcttggac acgtagtggc cgtcacagag atgtgcagga aattgccggg catggggatg
     3721 gccatgagcc ttggatatat ccccagcttt gtcgttgata tctcaggaac gaaggagcag
     3781 agagaaaagt atatacgccc gcttatagag ggcaggtatg tggctgcttt tagcgttaca
     3841 gaacctgatg ctggcagcga tatcagcgtt ttgaaaacca gaatggagga aaaaaatgac
     3901 tgttttgttg tgaacggttc gaagatattc acaaccaatg caggatatgc tgatttctat
     3961 acactccttg ccatggatgg aaatagcttt acagctttgc ttttcgataa aacagtgatc
     4021 gatgacaatg gccccaggcg ttttgagata aatgatcttg gcaggaagat gggaattaac
     4081 acaaccatgt caggggaact tgtcttgagc gattttcacg taccgaagga gtgcgtcctc
     4141 ggcgagcggg gtaaaggtaa aaaaaccgca cttgctgcat ttgaactcag cagggtagtg
     4201 attgcagggc aggcatttgg cggtatgctt tacggctatg aaagagcggt agattacgca
     4261 aagaagagaa agcagtttgg aaactccata ataaaattcc agtcaataga gcatgatctg
     4321 gtagatatgt atgtagaaat agaaaaatgc cgaaatcttc tatacaagac agcctggctc
     4381 tacgacagga aagatcctga gtcgaataaa tatgcctcca tggtaaaact gaccataccg
     4441 gaaaacgcga tttcggtttt gaacagatgc atagatgttt ttggtggcta tggatatatg
     4501 cgtgaccagg ggcttgaagg agcactcagg gatattcgta taacctcgat atatgagggc
     4561 accaacaaga tacagaagaa tgtcatagca agaatgcttt actgattcag tgcctgcttt
     4621 cgaggaaagc ctgaagaatt gtcccgttat ttccgattac tccctgtatg aaccggcgaa
     4681 atcgacaacg cacatagtcc tgtaagattt atgggggccg catgcaacgc ccacgacgag
     4741 aaagtctctt tcaaagatat atttctcacc ttctttgcca gcagcaccct cgtcaatagc
     4801 catctttgcc acgagggcag cgccttccaa atacccgtat cccagatatt caccaaactt
     4861 gcctcctggc tggccgcagc agttcattcg ctcgagtgtg gattttccat ccttatccct
     4921 atgaccggtg aggccctccg gtccacgatc acttactaaa ttacgggctg ccaggctcaa
     4981 tccttttgat gttctgaagg ctggtaaggg ttcctttttt tcaacgaaaa ggattgtctc
     5041 ctccaatcct tcactccact gaggactccc tctcaagctt ttcagaaact cggcgtattg
     5101 ccgggggttt gtcctgatag cattcagttc tacaatgact tctttttcca tgttggtcag
     5161 gaaggatgca tctctcaccc ctgtattgag cgggatccgt gagtcaggta tgtctacggt
     5221 caccacaaga ggaacagagc atgcccatat acctgcagcc acgatcagaa taaggaacac
     5281 cttaaaatac tgaacagtca ttttgagtca cctctcctta tgggtaatcc acccactcct
     5341 tccaaacgtg tggggcatac ccgaccgttg ccttgcccct gtttcttaca atgggggttt
     5401 taaacaaaag cggtttgctc attaactctt cctcgacgtt gtgagtgaga tattcgagat
     5461 ttagcctttt atactccttg ccttcgcgat caatgagagc atctatgccg aaaactgctt
     5521 ttacagaatt cagttctccc tgactcagcc ctttttctgc tagatttacg aactgaaagg
     5581 gtatgcctct ctccttgaaa tacctctgcg ccttttttgt ttcctggcat ttaagggtgc
     5641 caaaaatctg gatgtttatg cccaccgggg ttcacgaccc ctttaacagt ggcttcagct
     5701 atggcccggt gctgaatggg cagaaccgcc tggtgttact cctttggagt ttccagaaca
     5761 aaatgcagaa aagatttttt cagggttgtc tgcactgcat tttggacagc acagctcttc
     5821 attgctttct ccaatctttt gtaatacctc aaatctttcc ttgcagcttc tgcattcgta
     5881 ctcatacatt ggcatggttt tgtcctccat tggtgaagtc agatcatgat atatagtata
     5941 ttcaagaagc tgcaaaaaag aaagagcgga gatattcgag ggatgagtgg atactgttct
     6001 gcaaatccaa aaaaagacgg aggaaaaaac tctccagaaa gtaccgacat aaggtttctg
     6061 aatgacctgg ggtatactat gagagaaaca ggagtaatgg tttgactgga agaaagccct
     6121 gccaggagtt ccatctcacc ccgaggggct ggatttccgg tacacgcatg gaatgtggag
     6181 ttatccaggg tgaaataata gatcgtcccc ctgaggcgat cgaaacctgg ctggaggaaa
     6241 tggttctgtc ctatcttcac tgtgctgatg tgtattcgtg gcgcctaatc tggtttgatc
     6301 ccacaatgac agagggagac cgaaagaaga taagatcacg gtttgagaag ccttcagatg
     6361 agtttcctgg ctgaggttac ggggctatta attgttgctt caaataaatc gggatcattg
     6421 atgtgcaaac cagccgtata catggtacaa tgggcaaaaa tcagaccgtg atgaggagac
     6481 agaacatgtc gaagcttttc gaaacaacaa ccataaaaaa cctgattctg ccaaaccggt
     6541 ttgttcggtc agctacctgg gaaggcttgg ctgctgatga tggggctgtt actcaaagat
     6601 tgattgatgt tgctgtaagg cttgctctgg gtggggttgg gctgattatt tccggccacg
     6661 catatataag cagagaaggc caggcagggt tatggcagct cggtgtctat tctgatgagc
     6721 ttatcccggg cctgacagaa atgacaaagg cggttcacca gaggggagga aaaattgcca
     6781 tgcaactggc ccatgcaggc tccaaggctg catcacatct aagcgggctc gatcctattg
     6841 ggccatcaac tgcagaaata ggaggcagcc acattggaag ggagatgaat aaaaaagata
     6901 ttgagaaggt tatccaggca tttgctgctg ctgcgcttcg ggcaaaaagg gcaggttttg
     6961 atgcaatcca gattcacggg gcacacggat accttgtgag ccagttttta tcgccatact
     7021 tcaacagaag aacagatgag tatggaggaa gtatccagaa caggacgcga ttcgtagtgg
     7081 agcttcttga agcagtgcgt aaagcagtgg ggagtgaatt ccctgttttt ataaagctca
     7141 actcggaaga cttcttaccc aatgggctca cagtggatga aatgatcagt gcctctgaga
     7201 ttcttgagaa ggcgggcatg gacagcatcg agatgagcgg tggcaccttt ctctccggga
     7261 aaaactatcc ttcacgtcag ggcaagcccg gccaaggcga acatgaagca tattatgagg
     7321 ctgcagcaag acgatgtaag aagacgctta aaattccgct tatgctcgtg ggtggtttga
     7381 ggacttttga aacagccgag aggttggttt tggagaatgt ggcagactac atagccatgt
     7441 gtaggccttt cataagggaa ccagagctcg tgaaccgctg gaagtccggt gacaggaaac
     7501 cggctttgtg tatttcagac agcggatgct ttaggcctgg ttttgaaggg aaaggtgtgc
     7561 actgtgttgt tgaaggtcgt cttacgtctt aaacaatcgt gtgtgttcca tcttgatgca
     7621 aagattctgg acaaaatcta tgccgttcgc ctctgcaatt gcccttgctt catcgttgat
     7681 tatgtcaagc tgaagccata ttactttggg tttcagtttt attgcctcct taacaatggg
     7741 aacggtatgt tctgcctgca taaaaatgtc aacaacatcg actttttcca atatgtctgt
     7801 taatgatttg tacgattttt caccgagtat gttttcgtat cgcgggttaa cagggattac
     7861 cctgtagcca tgctccatca tgtaccgtgc aacacggttg ctcggcttgt tgtttcctgg
     7921 tgaaagcccc accattgcaa tggtttttgc tgataagaga atctcttttt ttttctcgtc
     7981 cgaaagctcc atagcgacct ccaaagatgc acgacctttt tctatcttga aaaacagatg
     8041 tcattgatta tactttaata cctatgaaaa aacaaaattc aacaggaggt cgaatttatg
     8101 atgcatgtca ggtttaacag gaatgtgatg ttgatgtgtg gcttattgat tgcacttggt
     8161 attattttga gccctgtgct ggcaagcgct caagcaacgg gtaaaggggt caagtttgaa
     8221 acatatcgga cagccataaa gaaggactat ggcatagaca taaaggattt caaggaccag
     8281 ctaaaaggtg gaaaggctga tggtaaagat ataaccaagt atgatctgaa acagttactt
     8341 atgggtatta aaaccgaaca ggaacatact acgaataaaa tgattgcact ggaaataact
     8401 atggatcacc ttgaaaaaat tccagactac tatgaccgat tggccgagat ggaagaagaa
     8461 gctctggatg acctcgaaga ctaaagggca gattcagaaa aaatagggaa gagaaaagat
     8521 cagaccgtta tttcagtagc tcgagagcat tgacaatatt gtcaatgccg acaaacggct
     8581 gtttttctgt tccattatca atgatgatgg tgggggttgc tcttacccca tcgtctttga
     8641 tgtattggct cattgcattg aatgtctgtt tagggtcaaa aggcttaaag cctatccctt
     8701 tttgggtgag aacttcttcg agtttttcct tggcggtgat tttttggctt gctgcttcaa
     8761 agaggaccct tcttgcagat aaggcgtgct cgaagtcttt tttgagattg agtatgtaga
     8821 gaaaatactg tgcatacaga gaagttgtct tatgagcagg cgtatcaata aacatgagct
     8881 ttatcttgtt tgtttttacc agttgcagaa gcagggcctc aacttttggc tcgccggcac
     8941 ggcacggtcc gcaaaaataa tctgtataaa cacggacgat tgtcttaccc tggccatagg
     9001 aaggaaggag cactccctgt gcgtaaagaa aactgggcct cagcggaata cagattgcaa
     9061 ggagcagggc agtaactgct atagcagcgc agatggtatc cggtgatctc ttcacggatt
     9121 ttaatatacc caaaatatgg tctggtgtaa agtgggtatt gtcacatcag gaagatcttc
     9181 ccatggtttg caatattgac agggggaagt ttggctgata ccataaaaga taatctcgga
     9241 tttttgacgc ttgattatga atttgaaaat tgaactccta aatactgata actgatagct
     9301 caaagcttat ttctcagggg gtaatgaatg gaagatcaat ttggcaggga cccgcaggtc
     9361 agatacttaa ggcgggtctt tgccggaatg gaacagaagc aaaaggaact tttggagcgg
     9421 ataggtctta ttcctacaga ttaccggctg aaacgaattc gtgaggcagc actgaaacac
     9481 ttcgagaagg cctggatgct tgcaggcagg aggggaatgg tagaaaccga ggacgagata
     9541 gcaggcctgt acacacattg tcttgcaaga gttctttcag gaaaccgtat cagtgtccca
     9601 acagaattac ttccctcaaa tgaaaagatt aatgaaatta tccaggaggt gttcaaatga
     9661 ttcgtttgcg cctcttcgga cgatgccgaa tatatcatga ccctgtatca ccggttctgc
     9721 gtgccccagc gcagattgga tgggatgcct ggtttcgttc aatcgatctt gttacccccc
     9781 aacccctcaa gggagaagag ttgctgagga gaacacgggg gtggtggaca gttgaaccga
     9841 cggaggttgc cgaggtcgta aaaaagaacg gccgccttgt tgttggtgaa ggcggggagt
     9901 taatggtgga atttgaggat gaacaatcag tccaggcgtt atcaacggcc ctgaaagaaa
     9961 agtttggtga tcaggttcag ctcgcaccgt aatgagcgct gagcgctcag catgattctg
    10021 tttttctcgg tttttggcta tgagctgaca ggtatcagct aaaaaagcta tttctcagca
    10081 tcctgccttc atgtttgcat caagataata ctcaagggtt tttctcagag cagtatccag
    10141 gtcaactttt ggctcccatc caatgagcgc ccgcgctttt ctgattgatg gtacccgaaa
    10201 atttatgtcc tgataaccat ctccgtagaa atcctttgaa aatacttctt taatctctga
    10261 atgtttctta taatggcccg tggagggatg gtttacaaaa atatctttca acttgtgcgc
    10321 aagctctttc acagtagctt cgttcttcgg gttgccgata ttgaatatct caccgttgaa
    10381 cttgttctct ttattttcaa tgatcttaac gaggcattca ataccgtnnn nnnnnnnata
    10441 taggtgaagc atcttttttg tagtcccccg tcaacaagac agattggttc actcttaatg
    10501 aggctaccga taaactgggt tacaacacgg gaactgcctt ctttttccgg atcggatgtt
    10561 aattcatcca gttttgggcc gatccagtta aaaggcctga aacacgtgaa cctgagccct
    10621 cgctcaaagc catatgccca gatgacacgg tcaaggagct gtttggaagc actgtaaatc
    10681 cagcgctgct tccttatggg gcccaggacc aggtggctct catcttcatc gaactccgtg
    10741 tctgtgcaca tgccatagac ttcggatgtg gagggaaaga gaagtctttt gttgtatttt
    10801 acacagagcc ggacaatggc gaggttttcc tcaaaatcta actcgacgac cttcagaggc
    10861 tctcttacct atgtagccgg tgttgcaatg gcaacaagag gcattacaac atcgcacttc
    10921 ttaacatggt actcaatcca ttccttgtta atgccaatat cgccctccac gaagtgaaag
    10981 tgattgctcc cgagacatgc atcaagcttg tcatttctga tatccattcc gtacacttcc
    11041 cagtctgtat cgctcagaat cttggccaca aggctgttgc caatgaatcc atttacgccg
    11101 agtaccagta gcttcacgtg tttcctccaa ggattttatt ttctacatca tatttttccg
    11161 caaaggcctc gccatccatt tcgtccccgc catccagttg caccctgtgg agtgtaagcg
    11221 tccctttgcc agtgctgaca gcaagaggtt tggttgaaat gatggaacca ggtaagc
//
LOCUS       DAOOMC010000091        11259 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdus sp. isolate AMR_MDS_2685
            NODE_6495_length_11259_cov_5.542842, whole genome shotgun sequence.
ACCESSION   DAOOMC010000091 DAOOMC010000000
VERSION     DAOOMC010000091.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864760
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdus sp. (wastewater metagenome)
  ORGANISM  Syntrophorhabdus sp.
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae; Syntrophorhabdus.
REFERENCE   1  (bases 1 to 11259)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11259)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,729
            Genes (coding)                    :: 2,708
            CDSs (with protein)               :: 2,708
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 7 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11259
                     /organism="Syntrophorhabdus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6495_length_11259_cov_5.542842"
                     /isolate="AMR_MDS_2685"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2699756"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(499..1512)
                     /gene="gap"
                     /locus_tag="PK125_09755"
     CDS             complement(499..1512)
                     /gene="gap"
                     /locus_tag="PK125_09755"
                     /inference="COORDINATES: protein motif:HMM:TIGR01534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HPB38426.1"
                     /translation="MPVRVAINGFGQIGRSVFRIGRVHPELDFVAVNDRVDSHTLAHL
                     LKHDSIQGRFTDEVKSTGTSIIVGSREIPVFSRNAPEKLPWKDLCVDVVLESTGMFTS
                     RDDAYKHIVAGAQKVVISAPGNEPDVTIIPGVNENQYNMHHHHVVSMGSCTANCLAPI
                     TKALHDAFGIVYGYMTTVHSYTNNQVLLDSPHKDLRRARTMAQSIFPTTTAAIEVIHK
                     TIPELTGRLEGISVRVPTPTVALVDFVATLSKRTSKEEVNECLKTAADGPLKNIMDCI
                     HDPFVSADFQGSPYSSIVDLPCTQVIGGSMVKIIAWYDNESGFSHRLCELFSLLLNEH
                     AIA"
     gene            complement(1643..3223)
                     /locus_tag="PK125_09760"
     CDS             complement(1643..3223)
                     /locus_tag="PK125_09760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate-forming enzyme family protein"
                     /protein_id="HPB38427.1"
                     /translation="MSDIQIHIGEILARNARMYPDDIALVERIAATKTRIEITWKDFD
                     ERANKFSNILMEKGIKKGDKVAHWMQNSIDWLIAYFGIVRTGAWVVPLNFRFIAEEMK
                     YCVDVAEPNVFVFDEEFTDRIGAVKEQLATVRSYICVGKNIPAFAESFHALMEKADVT
                     PLNTPLTFTDPCGLYFTSGTTGQPKPILLTHNNMANACIVENVHHRQTKEDVFILIPP
                     LYHTGAKMHWFGHFIVGSKAVILRGFSPEWVFEAISEEGGTVLWCLVPWVQDILIKLD
                     KGEMKLSDYKIDQWRLLHMGAQPIPPALVKHWRKYFPTMQYDTSFGLSESTGPGCVDL
                     RIGNEHKIGAIGLPGFNWEYKVVDDDGNIVPRGTPGELCVRGNGVMREYYKNPEATAK
                     ALKDGWLFTGDMVRVDEDGFIWIVDRKKDVIISGGENIFPAEVEDFLHTHPAVKDVAA
                     IGMPDERLGEIVAVVVDIIPGKTLTEEEILKHCEGLMKYKRPRKIFFGEIPRNPTGKI
                     EKVKLRKKYTGRGEAFKL"
     gene            complement(3257..4099)
                     /locus_tag="PK125_09765"
     CDS             complement(3257..4099)
                     /locus_tag="PK125_09765"
                     /inference="COORDINATES: protein motif:HMM:NF013757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HPB38428.1"
                     /translation="MEKKEEKSKSSYTGVVPAVDQASRILICLARNPSFKMNLTDICR
                     SVAIHKSKGYSILKTLQRYGFVHKDALGKTYSLGFGLISLSRKVLERLDYESIAGSHL
                     KKLADQTHSTALLGIIDDMNVFVVAKQEADQAIGVTTRIGHRFSISHGAHGKAIVSFL
                     PKHDQERILQQGRLFFHGTAEKLDRKRLKDEIRQCSQKGYAVDIGELNPGINVIASPL
                     FDSQGSLFGSLFIMGTFSESRIPEYSSIVVNVAKQFSATLGADISQTYGAKNSGFKDR
                     GSKC"
     gene            complement(4204..6021)
                     /gene="iorA"
                     /locus_tag="PK125_09770"
     CDS             complement(4204..6021)
                     /gene="iorA"
                     /locus_tag="PK125_09770"
                     /EC_number="1.2.7.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR03336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043805 - indolepyruvate ferredoxin
                     oxidoreductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate ferredoxin oxidoreductase subunit
                     alpha"
                     /protein_id="HPB38429.1"
                     /translation="MKKILQGNEAIARGAWEAGVSVACAYPGTPSSEILTEIAKYREI
                     YTEWSPNEKVAVEVAHGASLAGGRAIACMKHVGLNVAADPLMTISYTGVKGGLVIVVA
                     DDPGQHSSQNEQDSRNWTRFGKVPMLEPGDSQECKDFTKIAFDISERFDTPVLLRGET
                     RVSHCDSPVELSDRKESTIPKGLHQKDTPKFVMVPMYARIKRAAIEERTASLAEYADA
                     EFPFNIAEINDTSVGFISSGAAYLYTKEVFPQYSFLKLGMVWPLPKKMIADFVKKVKK
                     IIIVEELDSFLETEIKSMNIKVWHGKDLIPAIGELTPTIVEQSLAKVVTGKKYVAPKV
                     RVKPDDLPRRPPNLCAGCSHRPLFYALKKLGLYVFGDIGCYALAVAPPLSAMHASTCM
                     GAGVGGAYGAGKVLGKEGLGKICAVLGDSTFLHSGITPLMDAVYNKGYSTTIILDNRI
                     TAMTGQQETAGTGFTVKGEPAPMVDYEELARAVGVKHIRKVDPYNIQETMEVVKEEVA
                     RDDASVIITINSPCMLLRRAKPLEKFKNPVYAISMDNCRGCKMCLEINCPAISWREAV
                     GQTKDGHKRKGTVYINPDQCVGCEVCIQICKYQAIMPDK"
     gene            6413..6976
                     /locus_tag="PK125_09775"
     CDS             6413..6976
                     /locus_tag="PK125_09775"
                     /inference="COORDINATES: protein motif:HMM:NF036525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HPB38430.1"
                     /translation="MNVIASAAARLYSTKGYRETSMEDVAVSAKLSKGGMYHYFSSKE
                     DILYFILSNFMDSLLEGFEQDLENIRDPEDKIRYIIRHHVEAYVAHMYSAKVLLNEAY
                     NLSALRISRIKSKEKQYFSTIAEALSLYVGHRLNKDILTVVTFNLLGMCNWIYSWYNP
                     KGAINSEQLSQVIFKNFTRGLFSLQVL"
     gene            7009..8628
                     /locus_tag="PK125_09780"
     CDS             7009..8628
                     /locus_tag="PK125_09780"
                     /inference="COORDINATES: protein motif:HMM:TIGR00641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HPB38431.1"
                     /translation="MKRQTDYGYEIKPVYTPKDVSDISYEKDIGDPGSYPFVRGYYPN
                     GYRDRIWTRRMTAGLGSSRRTNKVLKKYREMGQTQGMLVIHDRPSAMPIDADHPMGRR
                     EAGVLGWHGVSLLEFEELMDGIPLIGQSITLLGSSAMSCMRLAYIVALAEKRGIDLKE
                     VHGSVTEYPFCHTFGQTDHMPLDLNIKLWLDSSEFIIRNKIRMRCGILSQHFQESGGN
                     NAQALAVEIGQLKELLGLLVNERGLDFDDVGLLPYELVSIGSRFFEEIAKVRALRRMY
                     ARMARETFNAKTEKACQLLISVHTSGRTMTYQQPYNNIARCAIQTLAGAIAGCTAIDN
                     ACLDNAHAEPSAFAARMSLNTQHIISDETGVTDVTDPLAGSYFVERLTNEVEDAAYKI
                     LQQIEKEGGMIAAVKKGYIHNMMYESARKRQKEIETGERLIVGVNELIIPPEDDDDIP
                     IQSLEASDSEEIAKRIEKWKETRDMSILKDKIEQLYKDAKKDRNHNLMPAIIEAVKAY
                     ATAGEIMGVIRMAHGFHYDPLEMIDCPFKLS"
     gene            8673..9071
                     /locus_tag="PK125_09785"
     CDS             8673..9071
                     /locus_tag="PK125_09785"
                     /inference="COORDINATES: protein motif:HMM:TIGR00640.1"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HPB38432.1"
                     /translation="MGDRRRIKVLISVIGLDGHFTGAEIVSRYLMDAGMEVVYLGIYQ
                     TPEMIVSAAIQESVDVIGISSHASNYDQIGMLLDLMKQNGLNDIPVICGGTIPRQHAE
                     TLLKRGVSKIFPPQSSSESIVNYITSIVKR"
     gene            9075..10091
                     /gene="meaB"
                     /locus_tag="PK125_09790"
     CDS             9075..10091
                     /gene="meaB"
                     /locus_tag="PK125_09790"
                     /inference="COORDINATES: protein motif:HMM:TIGR00750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0015819 - lysine transport [Evidence IEA];
                     GO_process: GO:0015822 - ornithine transport [Evidence
                     IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HPB38433.1"
                     /translation="MVNNTVKKIVNGDVMTAAKLIRDIDDQVPETREILKALHGYTGK
                     AYIIGVTGAPGVGKSTIVDQMVRDLRLNGKTVGVLAVDPTSPFSGGAILGDRIRMQRH
                     SLDKEVFIRSLATRGQFGGLTQSTLSAIDVLDAMGKEYIIVETVGVGQGEVDIARCVH
                     TTVIVVVPGLGDHVQAIKAGIFEVGDIFVVNKADRPEAERAVTYLRSMIDLAKKKFKN
                     EGWEPSIIMAEALNNKGIIELLNEIEKHRRSKKVGDILVAGKRDSARIQLTEMIKSRL
                     IEETLNRITGSPEFEKYLDCIMERTKDPYTACDELLSAMVFGLSLDPCSAPNRTSNHP
                     DEKD"
     gene            10176..11228
                     /locus_tag="PK125_09795"
     CDS             10176..11228
                     /locus_tag="PK125_09795"
                     /inference="COORDINATES: protein motif:HMM:NF024771.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HPB38434.1"
                     /translation="MSTIRDISFRSVVRPLRVVFATAQGKKRLMQSIIVRVVLTDGST
                     GLGECPTSISLNRESTDVVCSMLHEMKQQFQGIPIQHYEPVIRRFRTEYSEYPMTISG
                     IEVALFRAGLAYKKLTEHAYWGGKSRVVQSDITIPFVTDRSFLEHWIRYASQREFKVY
                     KLKVSGNIEHDKKVLSEVHYMLRKAVEEFTLCLDGNEGYSKEAFCRFVDYIQANTIKI
                     EFFEQPLARNDYQGLKEIKELSPFPIILDETVITSSDAQRVAEEGIAHGVNIKIAKSG
                     IGESAAILAIAKKHKLKMMIGCMTESMIGLSAAVYLAAGTDVFDYIDLDSVFLLYHKN
                     RFGNLSLHGPCFEIQP"
BASE COUNT         3120 a         2481 c         2555 g         3103 t
ORIGIN      
        1 agtgtctcat attggtatag aaggcacccg tattatacaa atgcctccta caatttccac
       61 agatgagatg ggtgtgaagt tggtcattat tcaaaaaaaa caggctcagt aatctcagtg
      121 gagaccttcg gttgccttta gcatcagacg ctttgttgaa gcaaaaggcc aagcttcttt
      181 gcttcccgat ggtttgttta aagaataccg ttcattatgg taccataata acataataat
      241 tttttccaaa catcgaatag gagagccgga tttcttatgt acggaacctc acgagacact
      301 atagcgatct ttttcctttg aattccaata atatattttt tccgtatatg gcctgaacag
      361 agccatgccg aggttttgac aataatcttg aaactcgttt cttcattcaa gaaacccgtt
      421 ttgcctttta catggggagt actgtactgc ctgtttcttg taacttgaca tgatactcca
      481 tagtctaaaa cattgacttt acgctatggc atgttcattc agaaggagcg aaaaaagctc
      541 acacaggcga tgggaaaagc ctgactcgtt gtcataccag gcaatgatct ttaccatact
      601 tccgcctata acctgagtac aggggagatc aactatggat gagtatgggc tcccttgaaa
      661 atcagcagaa acaaagggat catggataca atccattatg ttttttaatg gtccatctgc
      721 ggctgtcttt aagcattcgt tcacttcttc tttgcttgtc cttttagata gggttgccac
      781 aaaatcgaca agggccactg taggtgtggg tacacgtaca gaaatgccct caagccttcc
      841 ggtcaattca ggaatggtct tgtgaataac ttctatggct gctgttgtag taggaaaaat
      901 tgactgggcc attgttctgg cccttcgcag gtctttatga gggctatcaa gaaggacctg
      961 attatttgtg tagctgtgaa cagttgtcat atatccatag acgataccaa atgcatcatg
     1021 gagtgcctta gttatggggg caaggcaatt tgcagtgcaa gaacccatag aaacaacgtg
     1081 atgatgatgc atattatatt ggttttcatt aacgccgggt atgatggtta catcaggttc
     1141 gttcccaggg gcagatatga caaccttttg agcgccggca acgatgtgct tgtatgcatc
     1201 atccctgctg gtgaacatgc cagtgctttc gagaacaaca tcaacacaaa gatctttcca
     1261 gggaagcttt tcaggggcgt tccttgagaa taccggaatt tcccggctac ccactatgat
     1321 agaggtgcca gtgcttttaa cttcgtcggt aaaccttcct tggattgagt catgcttcaa
     1381 caaatgggcc agcgtatggc tgtccacgcg gtcattaaca gcaacaaaat caagttctgg
     1441 atgaaccctg cctatcctga aaacagacct tccaatttgt ccaaatccat ttattgcgac
     1501 tctaacaggc ataatatgag aacctagttg tatgtcctcg cattgctaca cgaaggttga
     1561 tgttttgatt aagaacaggc aatcaaaaca ataaataaaa acaggttctg cttattgtgt
     1621 caatacccta tggtagagcc tgttatagtt tgaaggcctc ccccctgccg gtatatttct
     1681 tgcgcagttt caccttttct atcttaccgg tgggatttct tggtatctct ccgaaaaata
     1741 tctttcttgg ccttttatac ttcataaggc cttcacaatg tttcaatatt tcttcctcgg
     1801 tcaaggtttt gccagggatt atatctacca ccactgccac aatctcgccg aggcgctcgt
     1861 cgggcatccc aatagcagct acatctttca ctgccggatg agtgtggagg aaatcttcca
     1921 cctctgcagg aaagatattt tcaccgcctg aaataatgac atccttcttg cggtctacga
     1981 tccagataaa tccatcttca tccacccgaa ccatgtcacc ggtgaagagc cagccatctt
     2041 tcaatgcttt tgcagtggct tccggattct tataatactc acgcatgacc ccattgcctc
     2101 gcacgcagag ttcccctggt gtccctctgg gaacaatgtt gccgtcatca tcaacaacct
     2161 tatactccca attgaatcct ggaagcccaa tagctcctat tttatgctca tttccgatgc
     2221 ggagatcaac gcaacctgga cctgtagatt cacttaaacc aaagcttgta tcatactgca
     2281 ttgtggggaa atattttctc cagtgtttca caagggcagg aggtatgggc tgggcgccca
     2341 tatgcaggag ccgccactga tcaatcttgt aatcggacaa tttcatctct cccttatcca
     2401 gtttgatcag tatatcctgc acccatggta caagacacca cagaacagta ccgccttcct
     2461 cacttatagc ctcaaacacc cactcaggag aaaagcccct cagaataaca gccttgcttc
     2521 ctacgatgaa atggccgaac caatgcattt ttgccccagt gtggtatagt ggggggatca
     2581 ggataaatac atcttccttt gtttgccggt gatgcacatt ttcaacgata caggcattgg
     2641 ccatattgtt gtgagtgagc agtatcggtt tcggctgccc tgtggtgcct gaggtaaagt
     2701 atagcccgca ggggtcagtg aaggtgagag gcgtgttgag gggggttacg tcggctttct
     2761 ccatgagggc atgaaaagac tctgcgaagg caggaatgtt cttgcccaca caaatatagc
     2821 ttctaacagt tgccagttgc tcctttacag ccccaatcct gtcagtgaat tcctcatcaa
     2881 agacaaatac gtttggctcg gcaacgtcaa cacaatactt catctcctca gctatgaacc
     2941 tgaaattcag cggaacaacc catgctccgg tcctgacaat gccaaaataa gcgataagcc
     3001 aatcaatcga attttgcatc cagtgagcga ccttgtcgcc cttctttatc cctttttcca
     3061 tcagaatgtt ggaaaacttg tttgccctct catcaaaatc tttccacgtg atttctattc
     3121 tcgtttttgt tgcagcaatc cgctctacaa gggcaatatc atcgggatac atgcgcgcgt
     3181 ttcttgccag tatttcacct atgtggatct gtatatcact catcgttccc cctccttagg
     3241 tttaacttaa aaatattcag cattttgaac ctctatcctt gaaccctgaa ttttttgcac
     3301 catatgtttg tgatatgtct gcgccgagcg tggcagaaaa ttgcttggca acgttgacaa
     3361 cgattgagct atactccggg atacgagact ctgaaaatgt tcccataata aacagtgagc
     3421 caaaaagact tccctgggaa tcgaacaggg gagaagctat tacattaata ccaggattca
     3481 attcccctat atccacagcg tatccctttt gactgcattg tctgatctcg tcttttaatc
     3541 tcttgcgatc aagcttttca gcagtgccgt ggaagaaaag cctgccctgt tgaagaatcc
     3601 tttcctgatc atgcttaggc aaaaaggaga ctattgcctt tccatgggct ccgtgactta
     3661 tactgaaacg gtgacctatt cgtgtagtaa cgccaatggc ttgatctgct tcctgttttg
     3721 ccacaacaaa aacattcata tcatcaatga tgccgaggag ggctgtgctg tgagtctgat
     3781 cagcgagttt tttcaggtgt gagcctgcta ttgactcgta atctaatctt tccagtacct
     3841 ttctcgacag ggatataagg ccaaaaccga gggaataggt ctttccgaga gcatccttgt
     3901 gcacaaagcc atatctttga agggtcttca atatggaata acccttgctt ttgtgtatgg
     3961 caacgcttcg acatatatct gtcaggttca tcttaaacga tgggttcctg gcaaggcaga
     4021 tcaggatgcg ggatgcctgg tcaacagcag gaaccactcc tgtgtaactg gattttgatt
     4081 tttcctcctt tttttccaaa cctacccctt ctgtttccgt gcctcgttcc agtcccctct
     4141 ggaagatatt gttccttcag tctcagtttt tctctgaaca ccgaaggtta tgtgattcac
     4201 tttttactta tcaggcataa tcgcctggta cttgcagatc tgtatacaga cttcacagcc
     4261 tacgcattga tcgggattaa tatagaccgt cccttttctt ttatgcccgt ctttggtttg
     4321 accgacagct tctcgccagc ttatggcagg gcagttgatc tcaaggcaca tcttgcaacc
     4381 acggcagttg tccatggaga tagcataaac agggttcttg aacttttcca acggcttggc
     4441 acggcgtaac agcatacagg gggagtttat cgttataata accgacgcat cgtcccgcgc
     4501 cacctcttcc tttactacct ccatagtctc ctgaatgttg tatgggtcta cttttctgat
     4561 gtgcttaaca cccacagctc tggcgagttc ttcatagtcc accattggtg caggctcgcc
     4621 tttgacggta aatcctgttc ctgcagtctc ttgctgccct gtcatggcag tgatcctgtt
     4681 atcgaggatt atggttgtcg aataaccttt gttgtaaaca gcatccataa ggggagtgat
     4741 acctgaatgg agaaaagtag aatctccaag gactgcgcag attttaccga gcccttcttt
     4801 tccgaggacc tttcctgccc cataagcgcc tccaaccccg gcacccatgc atgtagaggc
     4861 gtgcattgca gatagaggag gtgcaacggc aagggcataa catccgatat ccccgaacac
     4921 ataaagtccg agtttcttga gggcatagaa aagaggcctg tgagagcaac cggcgcaaag
     4981 attgggcggt cttctcggga ggtcgtcagg ttttaccctt actttcggtg cgacatactt
     5041 tttcccggtc actacctttg caagggattg ttctacaatg gtaggggtca gctcgcctat
     5101 agcaggaatc aggtccttac catgccacac ctttatattc atagatttga tttctgtctc
     5161 aagaaatgaa tcaagttctt ctacaataat aatctttttt acctttttta caaaatcagc
     5221 aatcattttc tttggaagag gccagaccat tccgagtttc aaaaaggaat attgaggaaa
     5281 aacctctttc gtatagagat atgcagctcc tgaagaaata aagcctacag atgtatcgtt
     5341 aatctccgct atattgaatg gaaactcagc atcagcatat tctgcaagac ttgctgttct
     5401 ctcttctatg gcagcccttt taattcgagc atacatgggc accataacaa attttggggt
     5461 atctttctgg tggagacctt tggggatagt tgactccttt ctgtcgctta actctacggg
     5521 agaatcacaa tgggataccc tcgtctcgcc acgcaacagt actggtgtgt caaaccgctc
     5581 actgatatca aacgcaattt ttgtgaaatc cttgcactcc tgtgagtcac caggctcaag
     5641 cataggtacc ttgccaaaac gggtccagtt cctgctgtcc tgctcatttt gagagctgtg
     5701 ctggcctgga tcatcggcca cgactataac aagaccccct tttacgcctg tgtatgagat
     5761 ggtcataagc ggatctgcgg ccacattaag gcccacgtgt ttcatacagg caatagccct
     5821 tccgccggca agagaggctc catgggcaac ctcaaccgcc accttctcat tgggtgacca
     5881 ctctgtatag atctcacggt attttgctat ttcagtaaga atttcactac ttggtgttcc
     5941 aggataggca catgcaacgc ttacacctgc ctcccaggca cccctggcga tggcctcatt
     6001 tccctgtaga atttttttca ttcttcctcc tcctgtcccc tctccaattc gaacggcggt
     6061 tgtgtactgt caaatgcacc tgttatattc tgtatgcaga accaagtttt gtattgcgaa
     6121 aaatattatg aaattgcagt attgttgtca atgaaattca acaaagtttc caccgtgatt
     6181 ttttatctct gtcaaggaac ctagaagggg cctcgccagg atgtggaatt tgatatcagg
     6241 gttccctgcg ctgattaata gattcaccca tgctattttt tcttgacaca ggaactgcat
     6301 ttccggtact ttaatccaac cagtcggttt aatccgacca gatggattat taaaactatt
     6361 tgtaatgaaa gagaaatatt cggttaaaaa acatctcaaa acagacaata aaatgaatgt
     6421 tattgcatct gcagctgcca gactttacag cacgaaaggg tacagggaaa caagcatgga
     6481 ggatgttgca gtaagtgcaa aattgagtaa aggtggaatg tatcactatt tcagctccaa
     6541 ggaagacata ctttacttta ttttgtccaa ttttatggat tccctgttgg aaggttttga
     6601 acaggatctt gagaatatta gggatccgga agacaaaatc aggtatatca tccgacatca
     6661 cgtagaggct tatgttgctc atatgtactc tgctaaagtg cttctcaatg aagcatataa
     6721 cctttcggca ttgaggatat ccaggattaa atctaaggaa aaacaatact tctcaaccat
     6781 agccgaggca ctttcgctgt atgttggaca caggctaaac aaagacatac ttactgtagt
     6841 cacattcaat ctgctgggaa tgtgcaattg gatttattcc tggtataatc cgaagggtgc
     6901 catcaattct gagcaactgt cgcaggtgat ttttaagaac tttacccggg gcttgtttag
     6961 tcttcaggtt ctttagggtg tttgttcaat acggaggcat gaaaaaggat gaaaaggcag
     7021 acagactacg ggtatgagat taaacctgta tatacgccaa aagatgtttc agacataagt
     7081 tatgagaagg atataggaga tccgggatct tatccttttg ttcgcggata ttatccaaac
     7141 ggataccgtg accggatctg gactaggcga atgacggcag gtctggggtc ttcaagaaga
     7201 accaacaagg tcttgaaaaa gtacagggaa atgggccaaa cgcaagggat gttagttatt
     7261 catgataggc cttctgctat gcctatagac gctgaccatc ccatgggcag aagagaggcg
     7321 ggtgttttgg ggtggcacgg ggtgtcattg ctcgagtttg aagaactcat ggatggtata
     7381 cctttgatcg gtcagtccat caccctcctt ggctcttcag ccatgtcctg tatgaggttg
     7441 gcctatattg ttgcccttgc cgaaaagcgt gggattgact taaaagaggt gcatgggagc
     7501 gtaactgagt atccgttctg tcacaccttc gggcagactg accatatgcc tttggatctg
     7561 aatatcaagc tgtggctcga ttcttcggaa ttcattataa gaaacaagat acggatgcgc
     7621 tgtggcattc tgagccagca tttccaggag tctggcggca ataatgcaca ggcgcttgct
     7681 gtggagatcg gacagctcaa ggaactgctc ggattgttgg tcaatgaacg aggcctggat
     7741 tttgacgatg tgggccttct tccatatgag ctcgtaagca ttggttcccg gttttttgag
     7801 gaaatagcca aagtccgggc tctgcgaagg atgtatgcaa ggatggcaag agagacgttc
     7861 aatgcaaaaa ctgagaaagc ctgccagctg cttatatcgg tccatacatc agggcggact
     7921 atgacctatc agcagccgta caacaatatt gcacgatgtg ctattcaaac acttgctggg
     7981 gccatcgcag gctgtactgc aatcgataat gcatgtctgg acaatgccca cgctgagcca
     8041 tcagcttttg cagccagaat gtctttgaat acccagcata tcatctcaga tgaaacaggg
     8101 gtgacagatg ttactgatcc tctcgctggc tcatactttg tagagcgtct caccaatgag
     8161 gtagaagatg cggcatacaa aattctacag cagatagaga aggaaggagg gatgattgcg
     8221 gcagtgaaga aaggatatat ccataatatg atgtatgaat cggccaggaa aagacaaaaa
     8281 gaaattgaaa caggagagcg tttaattgtg ggagtgaatg agctgataat tcctcctgaa
     8341 gatgatgatg acatcccaat tcagtcactt gaggcatctg actctgaaga aattgcaaag
     8401 cgaatagaaa aatggaaaga aacgagagat atgtccatct tgaaggataa aatcgaacag
     8461 ttatataaag atgcaaaaaa ggatcgaaat cacaatctta tgccagctat tatagaagcg
     8521 gtaaaagcat acgccactgc tggtgaaatt atgggagtca tacgcatggc ccacgggttt
     8581 cattatgatc cgctggagat gattgactgt cctttcaaac tatcatagca gggagtaacg
     8641 gtagcatgtg ccaaaacagg aggaatgccc atatgggtga cagacgaagg attaaggttc
     8701 tcatttccgt tatcgggctc gacggacact tcacaggtgc agaaatcgtg tctaggtatc
     8761 tcatggatgc tggcatggaa gtggtctatc tcggaatcta tcaaacgcca gaaatgatag
     8821 tcagtgcagc tattcaggaa agcgtggatg taatcggtat tagcagccat gcatccaact
     8881 atgatcagat aggtatgctg ttggacttga tgaaacaaaa cggtttaaat gatatccccg
     8941 ttatatgtgg cgggaccatt ccgagacaac atgccgaaac tctattaaaa cgcggtgtgt
     9001 caaaaatatt tccgccccag agttcttcag agtcaatcgt caattatatc acgtcaattg
     9061 taaagagata aaacatggtc aacaatactg ttaagaagat tgtgaatggt gatgtgatga
     9121 ctgctgcgaa gctaatccgt gatatcgatg atcaggtccc tgaaacgaga gaaatcctca
     9181 aagcccttca tgggtacaca ggaaaagcct atataattgg cgttacaggc gcccctggag
     9241 tcgggaaaag cacaattgta gatcaaatgg tccgtgatct caggctgaat gggaaaacag
     9301 tgggtgtact tgctgttgac cctaccagtc cattcagcgg aggagcaata ttaggtgatc
     9361 gaattcgtat gcagagacac agcctggata aagaggtatt tatccgttcc ttggcgacca
     9421 ggggccaatt tggtggactc actcaatcta cgctgtctgc tatcgatgta ctcgatgcaa
     9481 tgggtaaaga gtatatcatc gttgagacag ttggggtagg gcagggtgaa gttgatattg
     9541 ccaggtgcgt ccatacaacg gttatagttg tggtgcctgg cttgggagat catgttcagg
     9601 cgatcaaggc tggaatattt gaggtaggag acatctttgt tgtcaacaaa gcagatcggc
     9661 ctgaggcgga aagggcggtc acttacttga ggtccatgat cgatcttgca aagaagaaat
     9721 tcaaaaatga aggttgggag ccctccatta taatggcgga agctttaaat aacaaaggaa
     9781 taatcgagct cctcaatgag attgaaaaac acagaaggag taagaaagtc ggtgatattt
     9841 tagttgctgg aaaaagagat tcagccagga ttcaactcac agagatgatc aaaagcagac
     9901 tcatagaaga aacattgaac cgcataacag gcagccctga atttgaaaag taccttgact
     9961 gcataatgga aagaaccaaa gatccataca cggcatgcga tgaactgttg tcagctatgg
    10021 tgtttggact ttcccttgat ccatgttctg cgccaaacag gacatcaaac caccctgatg
    10081 aaaaggactg atcacttggc acaggctgaa gagaaaaaaa cacctttttg tataagtgct
    10141 aagctcttaa agaaattgga tactgacaaa acagaatgag cacgatcaga gacatctctt
    10201 tccgcagtgt ggtgaggcca ctcagagttg tgtttgcaac tgcacagggc aagaagaggt
    10261 tgatgcaaag cataattgtt cgggttgtcc ttaccgatgg atccacaggt ttaggggagt
    10321 gtcctacgag tatctcgctg aacagggaga gtacagacgt ggtctgcagc atgttgcacg
    10381 aaatgaagca acagttccaa ggtattccta ttcagcacta tgagcccgtc atacggagat
    10441 tccgtacgga atactcagaa tacccgatga ccatctccgg tatcgaagtt gcccttttca
    10501 gagcaggctt agcttacaaa aaactgacag aacatgcata ctggggcggc aaaagcagag
    10561 tcgttcagtc tgatattacc ataccttttg taaccgacag gtcttttctc gaacattgga
    10621 tcaggtatgc atctcaaaga gagtttaagg tttataaact caaggtgagt ggaaacattg
    10681 agcatgacaa aaaggtcctg tccgaggttc attatatgtt gaggaaagca gtggaagaat
    10741 ttacattatg cctcgatgga aacgaggggt atagcaaaga ggcattttgc aggtttgtag
    10801 actacattca agcgaatacc atcaaaattg aattcttcga acagcccctg gcaagaaacg
    10861 actatcaggg attaaaagaa ataaaggaac tatcaccctt cccgatcatc cttgatgaaa
    10921 cagtcattac atcatcagat gcacaacgtg ttgcagagga gggtattgcc catggagtaa
    10981 atataaaaat agcaaagagc ggtatcgggg aatctgcagc catcctggcc attgcaaaaa
    11041 aacataaatt gaagatgatg attggttgca tgacagaaag catgataggc ctgtcagccg
    11101 cggtttatct cgcagcagga accgatgttt ttgactacat tgatctcgat agtgtttttc
    11161 ttctttatca caagaacagg tttgggaatt tgtcgctcca cgggccttgt ttcgaaatac
    11221 agccgtaaaa gaaaaacctg ctttactgtt aagattgtc
//
LOCUS       DAOOMC010000092        11017 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdus sp. isolate AMR_MDS_2685
            NODE_6688_length_11017_cov_5.701970, whole genome shotgun sequence.
ACCESSION   DAOOMC010000092 DAOOMC010000000
VERSION     DAOOMC010000092.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864760
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdus sp. (wastewater metagenome)
  ORGANISM  Syntrophorhabdus sp.
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae; Syntrophorhabdus.
REFERENCE   1  (bases 1 to 11017)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11017)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,729
            Genes (coding)                    :: 2,708
            CDSs (with protein)               :: 2,708
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 7 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11017
                     /organism="Syntrophorhabdus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6688_length_11017_cov_5.701970"
                     /isolate="AMR_MDS_2685"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2699756"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..718
                     /locus_tag="PK125_09800"
     CDS             <1..718
                     /locus_tag="PK125_09800"
                     /inference="COORDINATES: protein motif:HMM:NF014195.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009427 - bacterial-type flagellum basal
                     body, distal rod, L ring [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="flagellar basal body L-ring protein FlgH"
                     /protein_id="HPB38435.1"
                     /translation="ILILISIFFVVTTGCNTVTQLEQSRIKGEPFILDNTFKYSKQDS
                     SKIRHGTNQGTIWRGENTASNYFIDQRAKGIGDIITIRITEVSAASEKATTGTGRTSD
                     INAGIGNFFGLDSKFSTISSHIDKNNLIKANTENTFSGTGETIRTGSLSATISAKVVD
                     LLPNGNLAIEGKREIYINNEKKEILLQGVVRPRDIAYDNSVYSTQVADAKVIYTGIGV
                     IGEKQRPGWMVRLFDIVWPF"
     gene            803..1924
                     /locus_tag="PK125_09805"
     CDS             803..1924
                     /locus_tag="PK125_09805"
                     /inference="COORDINATES: protein motif:HMM:NF014206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HPB38436.1"
                     /translation="MPSINCLIQKKLSLIIFVVLFFVNMTQNLYGARIKDLSYINGVR
                     TNQLVGYGLVVGLNGTGDKSNTIFTNQSLANMLEKMGIRIDPTKTKVNNIAAVTVTAD
                     LPPFGKVGNKIDATVSSIGDAKSLGGGILLLTPLKGADGEVYAIAQGPIVVGGFLATG
                     TGATVQKNHPTVGRIANGVTIEREISYADMKTDSITVSLKTPNYTTARRVVDRINQSF
                     AGAAYAKDSGTIAVKTPDELKKDVVRFVSIIENMDVKPDNTAKIVVDEKTGTVVMGDN
                     VLISTVAVSHGNITVQVKEDTNVSQPMPLARGRTVATPDTQIKVEEDKGRWVIIPEGI
                     TIKELVSAMNATGVPTRDIITILQTIKAAGALYAELEVI"
     gene            1943..2266
                     /locus_tag="PK125_09810"
     CDS             1943..2266
                     /locus_tag="PK125_09810"
                     /inference="COORDINATES: protein motif:HMM:NF021638.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod-binding protein"
                     /protein_id="HPB38437.1"
                     /translation="MSDILKLTKPMIGPDLVKHGQDITNVREISKNDQEKFEKIAEDF
                     EALFLFNMLKELDKTTKLSEKGYMEETYMSVIYDKVSQFLAKNKGIGIKEMLMRYSER
                     GDIKI"
     gene            2311..2616
                     /gene="flgM"
                     /locus_tag="PK125_09815"
     CDS             2311..2616
                     /gene="flgM"
                     /locus_tag="PK125_09815"
                     /inference="COORDINATES: protein motif:HMM:TIGR03824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis anti-sigma factor FlgM"
                     /protein_id="HPB38438.1"
                     /translation="MKINNEVKNSLLESLTKSKNVRASQEMDVQKGQGGGVADKVELS
                     GKRDEINRIKERIKAEPVVRQEKVDSIRDAIETKTYNIKGQLVAKSILKNHLLDQIL"
     gene            2619..3047
                     /locus_tag="PK125_09820"
     CDS             2619..3047
                     /locus_tag="PK125_09820"
                     /inference="COORDINATES: protein motif:HMM:NF016985.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlgN"
                     /protein_id="HPB38439.1"
                     /translation="MGEEILIEVTKKELYTLRQFLKILREERDAIISFSLEGIIRENN
                     RKEEVLRRLEYLEKEKEAFLENVPDRQALLEREPFKPLHREIHQVMEEVKVALEKNMK
                     LLSFSMDHVKSSIDNIVGFLNKTTYGGKREKISLLLSKEI"
     gene            3048..4451
                     /gene="flgK"
                     /locus_tag="PK125_09825"
     CDS             3048..4451
                     /gene="flgK"
                     /locus_tag="PK125_09825"
                     /inference="COORDINATES: protein motif:HMM:TIGR02492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
                     /protein_id="HPB38440.1"
                     /translation="MSIASILNIAKSAMFAQQASIQISANNIANVNTKGYCRLDPDLR
                     EAPLIPTDYGLLGNGVEIAGLVRYFDKFLEATIAQRNTDLQEQKTAVAYFERIESILS
                     EDSSNLATNVMEFFNGWHELSADPANLTRRSNLAAKGANLARTIRNLYAELSGLQREL
                     DQQVQREVDEINRLTASIADLNGKIIALGSSSQSEASDYLNQRTELLKELSGKIKITS
                     FEDENGGLTILTENGSALVDRTRSWKLQTQFNSDTGFRMIAWNDGTGNLSDITNKISS
                     GRVKAILDMRDKYLGNGFIADLNNLAGTIITEVNNLHKEGYGLNGTDKVPFFQETTTD
                     FARDMDLSMQIKEDVRYIAAALSSDDYSGNDNALRIASLIDTDVVIDGQNTRFSTFIS
                     TIMNNIGGLTKAAQQLSEHEESSMKIMEQQRESRSGVSIDEELAKLMKYQYAYQAAAR
                     LISVADEMFQTMLGVGR"
     gene            4448..5938
                     /gene="flgL"
                     /locus_tag="PK125_09830"
     CDS             4448..5938
                     /gene="flgL"
                     /locus_tag="PK125_09830"
                     /inference="COORDINATES: protein motif:HMM:TIGR02550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgL"
                     /protein_id="HPB38441.1"
                     /translation="MRIADRFKYDLFMYQFNNVKTSLDRIQEQMAKQKRVLRPSDDPV
                     AYAISVDLRAESVIYDQLERNILRVTTFGKVYDTVFGTMKDLLAEAKSVAINHAAGSM
                     DDALRKNAVKQVESIIEQLVALGNTVVGDTYVFGGIKSNSAPFRLNPDYSVDFIVPEG
                     AEQGNDIFIDRGNKAQYTISGRDAFYNRSKTIYESPNNKYRGEMILNTTDLAYVVDGT
                     NNTIYRNGSAITLTQGTYTGATLASEIQTRLNELETGHAVTYDASTRRFTFANNTTNN
                     ITLDWSNPGSTAGQMLGFSAVDATVLANGGWDKSDVDTGTTSFQVEILDNGYYQYSVN
                     GGAMSGPVAINGGTFIDGSTEANAINRGIRITFITTEGLTEGDSFEIKDYSIFEMLKN
                     LRDTLSDPLSNNANWSRTNIAKIDEGLNIIRRNTAYVGTNLQTMDRLTEANNARQNRT
                     AKIISDTMDADLAQLATEYSNLSTIYQSLMYSFTKIQELGLLNFLK"
     gene            6172..7167
                     /gene="pseB"
                     /locus_tag="PK125_09835"
     CDS             6172..7167
                     /gene="pseB"
                     /locus_tag="PK125_09835"
                     /EC_number="4.2.1.115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016292049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 4,6-dehydratase
                     (inverting)"
                     /protein_id="HPB38442.1"
                     /translation="MFDDKVILITGGTGSFGQKFTGYLFEHHKPRKVIIFSRDEFKQH
                     QMAKIFPDSEYAIRFFLGDIRDKERLYRSFEGVDYIIHAAALKQVPAAEYNPFEAVKT
                     NIIGAQNIIDAAIDRGVKKVVALSTDKAVSPINLYGATKLAMEKLFIAANSYVGLKET
                     SFALVRYGNVIGSRGSVIPLFNGMKNNGVKKFPITDERMTRFWITLDQSVSLVAYAVA
                     ESVGGEVYVPKIPSMRILDLINAISDDAQYEVVGIRPGEKIHESLVSEDDGRNTVDLK
                     DKYVILPPFANGGNKYHRYTKFPRMTDGFSYRSDNNVKWLTVKEIEQFIEECECE"
     gene            7160..8341
                     /gene="pseC"
                     /locus_tag="PK125_09840"
     CDS             7160..8341
                     /gene="pseC"
                     /locus_tag="PK125_09840"
                     /EC_number="2.6.1.92"
                     /inference="COORDINATES: protein motif:HMM:TIGR03588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-4-amino-4,
                     6-dideoxy-N-acetyl-beta-L-altrosamine transaminase"
                     /protein_id="HPB38443.1"
                     /translation="MNKVVPYSRQYIDQDDIEAVISVLESDYLTQGPAIGKFESAIRE
                     YTGAQYCAVFSSGTAALHASYFAAGLDKGDEIITSPITFVATSNAALYLGARPIFSDV
                     ESDTANLNVGSLEEKIGHKVKVIVPVHFAGHPVDMVAVRALADKYKLTVIEDACHALG
                     AYTKTGSNGWAKVGSCQCSDMTILSFHPVKHITTGEGGAVLTNNKLLFDRLLLFRSHG
                     ITKNRHDFVSTNNGDHGEWYYEMQCLGYNYRMTDIQAALGNSQLTKVEQFVRTRREIA
                     AHYNSVFMDNPFFHIPVERDYGKSSYHLYTIRLKDHLVNKRTAIFSKMRGEGLGVQVL
                     YIPVYLQPYYETLGYKNGCCPVAEDFYRRAISIPIYHGMTMSDCHRVVDTIFKIMDEV
                     N"
     gene            8344..9183
                     /locus_tag="PK125_09845"
     CDS             8344..9183
                     /locus_tag="PK125_09845"
                     /inference="COORDINATES: protein motif:HMM:NF014409.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family protein"
                     /protein_id="HPB38444.1"
                     /translation="MVGAIIQARTSSTRLPGKVLLELPYGSGITVLQQVIRRVKQSKM
                     IETIVVATTEDRADDAIADIASQEGVLSFKGSKENVLERYYLAAKTFGLTTIVRITSD
                     CPCIDASVVDETINRYIHEQADYCSISAAKTFPHGLDNEVFSFSALDMAYNNARKDYE
                     KEHVTPYIYRSNPEAFKILIIHAPQELHDPEIRITLDTEEDYAVLCCIYDNLYYKNTF
                     FNAKDIIQLFIEKPWMRIINKKIIQKKIFDTLEQEIDEALRIIELNDLKLARNAFEKC
                     LKE"
     gene            9201..10211
                     /locus_tag="PK125_09850"
     CDS             9201..10211
                     /locus_tag="PK125_09850"
                     /inference="COORDINATES: protein motif:HMM:NF015087.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016051 - carbohydrate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase family protein"
                     /protein_id="HPB38445.1"
                     /translation="MRIGDKTIGKGRTYIIADVGSNFNGSLELAKDYIRVGKEIGIDC
                     VKFQSYTAESLLSPFKPDGSKWEAYDVVKKYELPVSWHSVLKECADMCGVEFMSTPFS
                     LEIVDKLNEMGVRAFKVASGDLTFIPLLKKLGSCGKPIILSTGMAYTGEIDTAIKRLR
                     QSGTEDVALLHCVSNYPPKYEKVNLKAVETMRQTFNVPIGLSDHTLDDVTALGAVALG
                     ACIIEKHITFDKNLGTPDAPFAMTIDEFKAMINRIRNLEKALGDGIKEPADDEIAERQ
                     WARRGVYASRSIKVGEKLTLDNAKFLRPIRGISALEWMTYQESTIKRNIAGGEPVTRD
                     DM"
     gene            10281..>11017
                     /locus_tag="PK125_09855"
     CDS             10281..>11017
                     /locus_tag="PK125_09855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB38446.1"
                     /translation="MNMKGTILFRCDGSHEIGFGHIVRCIALADELRDKHGSGVVFAV
                     LYDNKAMEMILNHGYKVETANPREDQFDYGDWFNCIVKKNHPCALILDIRDDLPPNIV
                     SNLRKKGILVVTIDDPTDRRLLADLAFYPPVPQVEKMDWHGFTGQRFIGWEWVILRRE
                     FSHNDENLYKRIKATKKTKIHNILVTMGGSDPKGMTLKTMEALKVIDGQFEVTIILGP
                     GFAHRDSLNEILGNFPHIHRIFENVLN"
BASE COUNT         3571 a         2046 c         2556 g         2844 t
ORIGIN      
        1 aattttaata ttaatttcga tattttttgt tgtgacaacc ggttgcaata ccgtaaccca
       61 gttggaacaa tcacgcataa aaggcgagcc ttttatccta gacaatacct ttaaatattc
      121 caagcaggat tcaagcaaaa tccggcatgg aacaaatcag ggaacaattt ggaggggcga
      181 aaatactgca agcaattatt ttatagacca aagagcaaaa ggaattggtg atattattac
      241 aataagaatt actgaagtat ctgcggcgtc agagaaagca acaactggta cgggaagaac
      301 atctgacatt aacgcaggga taggtaattt tttcgggtta gactctaaat tttcaactat
      361 cagtagtcac atcgataaaa acaatcttat taaagcaaat acggagaaca ccttttccgg
      421 caccggagaa acaatcagga ctggctcgtt gagcgctaca atttctgcta aagtagtcga
      481 ccttctgcct aatgggaatc tcgcaattga aggaaagaga gaaatataca ttaacaacga
      541 aaagaaggag atactcttac agggcgttgt gaggccgaga gacattgctt atgataattc
      601 agtatattca actcaggtcg cggatgcgaa ggtaatatat actggtattg gtgttatcgg
      661 agaaaaacag agaccagggt ggatggtgag attgtttgat atagtgtggc cattttgaga
      721 ttctggatat ggttagcaaa tgaaggaaga aacaagagat aaaagtgttt acaataaatg
      781 ccatagacaa ggattgaatg acatgccttc tataaattgc ctgatccaaa aaaaactttc
      841 cctcattatc tttgttgttt tgttttttgt aaatatgacc caaaacctct acggagcgcg
      901 tattaaggat ctgtcatata tcaacggagt cagaaccaac caactggtcg gttatggatt
      961 ggtagtcggt cttaatggga caggcgacaa atccaacacc attttcacca accagtccct
     1021 tgcaaatatg cttgaaaaaa tgggtatcag aattgatccg acaaaaacga aagtcaataa
     1081 tattgcagcg gtaacggtaa ctgcagacct gcccccattc ggaaaggtgg ggaacaaaat
     1141 cgatgcaact gtatcgtcca tcggggacgc aaagagtctt ggcggaggca ttctccttct
     1201 tactcctctc aagggggctg atggggaggt gtacgctata gcccagggac ctattgttgt
     1261 gggaggtttt ctcgcgacag gaaccggggc aactgtgcag aaaaaccatc caactgtggg
     1321 acgaattgcc aatggagtta ccattgagcg agagatcagt tatgctgaca tgaaaacaga
     1381 ctctatcacc gtgtccttga agacgcccaa ttatacaacg gcaagaaggg tggtagatcg
     1441 catcaatcag tcctttgccg gggccgcgta tgcaaaggac agcggaacca ttgctgtaaa
     1501 aacaccggat gagctgaaaa aagatgtggt tagatttgta tccattatcg aaaacatgga
     1561 tgttaagcct gataacaccg caaaaatagt ggttgacgag aaaacaggca ctgtggttat
     1621 gggagacaac gttcttattt ctactgttgc tgtctctcat ggcaatatca cggttcaagt
     1681 taaagaagat acaaatgtaa gccagccaat gccattggca agaggacgga cagtggcaac
     1741 tccggacact cagattaagg ttgaagaaga taaaggccga tgggtcatta ttccagaggg
     1801 gataactata aaggaattag tgagcgccat gaacgcgaca ggtgtaccga caagggatat
     1861 aataacaata ctgcagacca taaaggcagc tggagctttg tatgccgaac tagaggtaat
     1921 ttagttgacc tggtgatgat ccatgagtga catattgaaa ttgacaaaac caatgattgg
     1981 ccctgatttg gtaaaacatg ggcaggatat tacaaatgtt cgtgaaatct caaagaacga
     2041 tcaggaaaaa tttgaaaaaa tagctgaaga ttttgaagct ctttttcttt tcaatatgtt
     2101 aaaggaactt gataaaacga cgaagttgtc ggagaaaggg tacatggaag aaacctatat
     2161 gtccgtcatt tacgacaaag taagccaatt tcttgcaaaa aataaaggta taggaatcaa
     2221 agagatgctc atgcgatatt ctgaacgtgg tgacattaag atttaagtaa aataggcgat
     2281 aagaggggta agtatcagag gaggaatgcc atgaaaatta acaatgaagt aaaaaatagc
     2341 ttgcttgaaa gcctcacaaa gtcaaaaaac gtcagggcat ctcaggagat ggatgttcag
     2401 aaggggcagg gtgggggtgt agcagataaa gtagaactat ctggaaaaag agatgaaata
     2461 aacaggatca aagaaagaat taaagctgaa ccagtagtaa ggcaggaaaa agtggatagc
     2521 atccgtgatg ctatagagac aaaaacatat aatataaaag gtcagctcgt ggcaaaaagc
     2581 attctgaaaa accacttgct tgatcaaatc ttataattat gggcgaagag atactgattg
     2641 aggttacgaa aaaagaattg tataccttgc ggcaattcct caaaattctc agggaagagc
     2701 gggatgccat tatctccttt tcacttgaag ggattatccg agagaacaac cgtaaagaag
     2761 aggtgcttcg ccgcctcgaa tacctggaga aagagaaaga ggctttctta gaaaatgttc
     2821 ctgacaggca ggcgctattg gagcgtgagc ctttcaaacc tcttcatcga gagattcatc
     2881 aggttatgga ggaggttaag gtggcattag agaagaatat gaaactcctc tcgttttcaa
     2941 tggatcacgt gaaatcctcg attgacaaca tcgtcgggtt tttaaacaaa actacatatg
     3001 gtggaaaaag agaaaagata tctcttttat tgtcaaagga gatatagatg agcatagcat
     3061 caatattgaa cattgccaag agtgcaatgt ttgcccagca agcatccatt cagatttcag
     3121 ctaataatat tgcaaacgtg aacacaaaag gctattgcag gcttgaccct gatttgcggg
     3181 aagcccccct catacctact gattatggtt tgctcggaaa cggtgttgag attgccggcc
     3241 tggttcgata ttttgacaaa tttcttgaag ctaccattgc acagaggaac actgacctcc
     3301 aggaacagaa gacagctgtt gcgtattttg agagaatcga aagcattctc agcgaagata
     3361 gctcaaatct ggcgactaac gtgatggagt ttttcaacgg ctggcatgaa ctctctgctg
     3421 accctgcaaa ccttaccaga cgaagcaacc tggcagcaaa gggtgcaaac ctggcacgaa
     3481 ccatacgaaa cctttatgct gagttgagtg gactccagag agaactggac caacaagtcc
     3541 agcgggaagt ggatgagatc aacaggctta cagcatctat tgctgatctc aatggaaaaa
     3601 ttatcgctct gggttcttcg tctcaaagtg aggctagcga ttatctcaat caacgcacag
     3661 aactgttgaa agagctttca gggaaaataa agatcacatc ttttgaagac gagaatggtg
     3721 gactgactat cctgactgaa aatggtagtg ctcttgttga tcggacacga tcatggaaac
     3781 tacaaacaca gtttaattct gacacagggt tcagaatgat tgcatggaat gatgggacag
     3841 gcaacctgtc agatattacg aataaaatca gttcgggcag ggtaaaagcc attctcgaca
     3901 tgagagataa atatctgggt aatggattta ttgcagacct gaataatctt gccggcacca
     3961 tcatcacaga ggtcaacaat ctccataaag agggttatgg cttgaatggt accgataagg
     4021 ttccattctt tcaggaaacg accactgatt ttgcaaggga catggatttg agtatgcaaa
     4081 ttaaggaaga tgtgcgttac atcgctgctg cattatcttc ggatgactat tcaggtaacg
     4141 acaacgctct gaggattgct tccctcattg atacagatgt tgtgatcgat ggccagaata
     4201 caagattctc aacatttatc tccactatca tgaataacat tgggggcctt acaaaggctg
     4261 cccaacaact gtcagaacat gaggagagtt ccatgaagat catggagcag cagagagaaa
     4321 gcagatcagg agtgtcgatt gatgaagaac tggcgaaact aatgaaatac caatatgcct
     4381 accaggcagc agctcggttg atttcagtcg cagacgagat gtttcagacc atgctagggg
     4441 ttggtcgatg agaattgcag acaggtttaa atatgatctt ttcatgtacc agttcaacaa
     4501 cgtaaagacc agcctcgacc gcattcagga acagatggca aaacagaaaa gggttttgag
     4561 gccttctgac gatcctgtag cgtatgctat ttctgttgat ttgcgtgcag aaagtgtcat
     4621 ctacgaccag ttggaaagga atatcttacg ggtgacaaca tttggaaaag tatacgatac
     4681 tgtatttggt actatgaagg atctccttgc tgaggcaaaa agcgtcgcaa tcaatcatgc
     4741 cgcaggttct atggatgatg cccttcgaaa aaatgctgtt aaacaggtag aaagcatcat
     4801 agagcaactc gttgcccttg gaaatacggt agttggagat acatacgttt tcggaggaat
     4861 aaagtcgaat agcgctccct tcaggttaaa tccggattat tcagtagatt ttattgttcc
     4921 tgaaggcgct gaacagggca atgacatatt catagaccgc ggcaacaagg cacaatacac
     4981 catatccggg cgggatgctt tttataatcg gtcaaaaacg atttatgaaa gtccgaataa
     5041 taagtaccgc ggcgaaatga tcctgaatac cacagaccta gcgtatgtgg tagatgggac
     5101 caacaacacc atttatagaa atggttcagc cattacattg acgcagggta catatacagg
     5161 ggcaacgctc gcatcagaaa tacaaacaag gcttaatgag ttagaaactg gtcacgcagt
     5221 aacgtacgat gcatcaacac ggaggttcac cttcgctaac aacactacca ataacattac
     5281 cttggactgg tcaaacccag gttccaccgc gggccagatg cttggtttta gcgcagttga
     5341 tgcaactgtg ttagcaaatg gcggctggga taagagtgat gtggacaccg gcacaacgtc
     5401 ttttcaggtt gaaatattgg acaacggata ttaccaatat tctgtgaatg gtggcgcgat
     5461 gtccggacct gtggctataa atgggggcac ctttattgac ggatctacag aggctaatgc
     5521 cataaatcgt ggtatcagaa taacttttat tacaacagaa gggttgacag aaggtgattc
     5581 ctttgagatc aaggactatt caattttcga aatgttgaaa aacctgaggg atactctatc
     5641 tgatccactc agcaacaatg caaattggag caggaccaac attgcaaaga tcgatgaggg
     5701 gctcaacatt ataagaagaa atactgcata tgtgggcact aacctgcaga ctatggatag
     5761 attgactgaa gcaaacaatg cccggcagaa tcgtactgcg aagattattt cagacaccat
     5821 ggatgcagat ttagcacagc ttgccactga atacagtaat ctatcaacca tataccaatc
     5881 cctcatgtat tcattcacaa agatacagga actgggattg ttgaatttcc tcaaataatt
     5941 ttttacacat ttattacttc caacataaat gcatcttctt aacctaccgg ccatttgagg
     6001 caaattgtac tggtatactt attgcattca atcacgaaga gttcttaaat ggtctataaa
     6061 tggctattct atagggtact ggaggagaca gaatggcttc cattgtcaag tcacggaata
     6121 aaggagcgta cgaatatttg aaagatattt gaccccaaaa ggagatggtt catgtttgat
     6181 gataaggtaa tccttattac tggaggcaca ggatcatttg gtcagaagtt tacaggctat
     6241 ttgtttgaac atcacaaacc cagaaaagtc attatcttca gcagggatga gttcaaacag
     6301 caccaaatgg caaagatctt tcctgattca gagtatgcaa tacgattttt tctcggagat
     6361 atcagagaca aggagcgttt gtacagatca ttcgagggtg tagactatat tattcacgca
     6421 gctgccctca aacaggttcc agcagcagag tataacccct tcgaagctgt gaaaacaaac
     6481 attattggtg cgcaaaatat aattgatgcc gcaatagaca gaggagtaaa aaaggttgtt
     6541 gccctgtcga ccgacaaagc tgtgagtccc ataaatctct atggtgcaac aaagcttgcc
     6601 atggaaaagc tatttattgc tgctaacagc tatgttggtc tgaaggaaac ctcatttgct
     6661 cttgttcgtt acggaaatgt tattggaagt cgtgggagtg ttatccccct gtttaacggg
     6721 atgaaaaata atggtgtgaa aaagttccca attaccgatg aacgtatgac cagattctgg
     6781 atcaccttgg accagagcgt ttcactcgtt gcgtatgctg tggctgagag cgttggaggt
     6841 gaagtctatg tgcctaagat tcccagcatg agaatacttg atctcatcaa tgcaatatca
     6901 gatgatgccc agtatgaggt tgtgggcata cgccctggag agaaaattca tgagagcctt
     6961 gtttctgaag acgatgggag gaacacagtt gatctaaagg ataagtatgt catcctaccg
     7021 ccatttgcta atggtggaaa caaatatcac aggtacacca aatttccgag aatgacagac
     7081 ggtttttcct atcggagtga caataatgtc aaatggctga cagtcaagga aattgagcaa
     7141 tttattgagg aatgtgaatg tgaataaagt tgttccttac agcagacaat acattgatca
     7201 ggacgacatt gaagcagtga tatcagtctt agaatctgat tatttgactc agggaccagc
     7261 aatcggaaaa tttgagtctg ctattcgaga atacacagga gcgcaatact gtgcagtttt
     7321 ctcctctgga accgcagcgc ttcacgccag ttattttgct gctggtttgg acaaaggcga
     7381 tgaaataatt acgtcaccaa ttacctttgt ggcaacttca aatgctgcgc tttacttggg
     7441 tgcaaggccc atattttctg atgttgagtc agacactgcc aatctcaatg ttggaagcct
     7501 ggaggagaag attggccata aggtgaaggt tatagttccg gtgcatttcg caggacatcc
     7561 agtagacatg gtggctgtcc gcgcattggc tgataaatat aagcttacgg tgatcgagga
     7621 cgcatgccac gccctggggg cttacacaaa aacagggagt aatggttggg ccaaagttgg
     7681 aagctgtcaa tgctcggata tgacgatcct tagctttcat cctgtcaaac acatcactac
     7741 gggagagggt ggagctgtat taaccaataa taaactgttg tttgataggc ttcttttatt
     7801 tcgcagtcat gggataacca agaacagaca tgatttcgta tcaacgaaca atggagatca
     7861 cggtgaatgg tactatgaaa tgcagtgcct tgggtacaac tatcgtatga ctgacataca
     7921 ggcagccctg ggtaactcac aattgacaaa ggtcgaacaa tttgtaagga cgaggagaga
     7981 gattgccgcg cattacaaca gtgtttttat ggataaccca tttttccata taccagttga
     8041 aagggactat ggaaaatcct cttatcatct gtacacgatt cgtttaaaag atcatcttgt
     8101 aaacaagcga accgcaatct tctccaagat gcgtggagaa ggtcttggtg tacaggttct
     8161 ttatatccct gtttatctcc aaccttatta tgaaaccctg ggatataaaa atggttgctg
     8221 tccggtagct gaagatttct acagaagggc aataagtatc ccaatctatc atggcatgac
     8281 catgagcgac tgtcacagag tggtagatac gatattcaaa ataatggatg aggtgaacta
     8341 acgatggttg gcgctataat acaggcacgt acttcatcta caaggctacc tggtaaggtg
     8401 ttgcttgagc tcccatacgg gagtggtatc acggtactac aacaggtcat tagaagggtc
     8461 aaacaatcca aaatgattga aactattgtc gtagccacaa cagaggatag agctgatgac
     8521 gctatagctg atatcgcctc ccaggaagga gttctctcct ttaagggaag caaggaaaat
     8581 gtactcgaac gttattatct ggcagcgaaa acatttgggc taacgacaat agtgcgaatc
     8641 accagtgact gtccgtgtat agacgcatca gtggtggatg aaaccattaa tcgttatatc
     8701 catgaacaag ctgactattg cagtatatct gctgcaaaga cttttcccca tggacttgat
     8761 aatgaagtat tttctttctc tgcccttgac atggcttaca acaacgcaag gaaagattat
     8821 gagaaagagc atgttactcc atatatatac cgatcaaatc ccgaagcatt caaaatattg
     8881 attatacatg cacctcaaga acttcacgac ccggaaatca gaatcacact ggatacagag
     8941 gaagactatg ctgttctgtg ttgtatttat gacaatcttt attataagaa tacatttttc
     9001 aatgctaaag acataataca attattcata gaaaaaccct ggatgagaat tataaataaa
     9061 aaaataatac aaaaaaagat tttcgacaca ttagagcaag agattgatga agcattaaga
     9121 attattgaac tgaatgatct caagttggca agaaatgctt tcgaaaaatg tttgaaagaa
     9181 taaacattta tgaggaaaag atgaggatcg gtgataaaac cattggtaaa ggaaggactt
     9241 acataatcgc agatgttgga agtaatttta atggcagttt agagcttgcc aaagattata
     9301 taagagtagg aaaagagatt ggcattgact gtgtcaagtt tcaatcatac acagcagaat
     9361 cattgttaag ccctttcaag ccagatgggt caaaatggga ggcctatgat gtggttaaga
     9421 aatatgaact tcccgtttca tggcattctg tgctcaagga atgcgctgat atgtgtgggg
     9481 tagaatttat gagtacccca ttttcacttg aaatagttga taaattgaac gagatggggg
     9541 tcagggcttt taaagttgcc tcaggcgatc tcacttttat ccctcttctt aagaagctgg
     9601 gtagttgtgg aaagccaatt atcttgtcaa caggtatggc atataccggt gagattgata
     9661 ctgcgattaa gaggcttcga cagagtggaa ctgaagacgt agcgctgttg cattgtgttt
     9721 ccaattatcc acccaaatat gaaaaggtta acctcaaggc tgttgagact atgagacaaa
     9781 cattcaatgt acctattggc ttatcggatc ataccttgga tgacgtgaca gctttgggcg
     9841 cagttgcttt aggagcatgc attattgaaa agcatataac atttgataaa aatctcggta
     9901 ctccagatgc cccttttgct atgaccattg atgaatttaa agccatgatt aacaggataa
     9961 gaaatttaga gaaagccttg ggtgatggga taaaagaacc tgcagatgat gaaatcgcag
    10021 aacgacaatg ggcacgtagg ggcgtatacg caagtcggtc gattaaagtg ggagaaaaac
    10081 tgacactcga caatgctaag ttcctgaggc caattcgagg aataagcgca ttggaatgga
    10141 tgacatatca ggaaagcaca atcaaaagaa atatagcagg gggcgaacct gtaacgagag
    10201 atgacatgta aattaagatc atatatcacc gaaaaaaggc aagcctctgt atcgatcaaa
    10261 gaaatttact ggaagatata atgaatatga aaggcactat tctttttcgt tgcgatggtt
    10321 ctcatgaaat tggttttggc catattgtgc gatgtatcgc cctggcagat gaactgagag
    10381 ataagcacgg cagcggagta gtgttcgcag tactttacga taacaaagca atggaaatga
    10441 tattgaatca tggatacaag gttgagactg caaatccccg cgaagatcaa tttgactatg
    10501 gagattggtt caattgtatc gtaaaaaaaa atcatccctg tgccctcatc ctcgatatac
    10561 gtgatgactt gccacctaat atagtatcca atcttcgaaa aaaagggatt ttggttgtaa
    10621 ctattgatga tccaacggat cgacgtcttc tcgctgatct tgccttttac cctcctgttc
    10681 cacaagtcga aaaaatggat tggcatgggt tcacaggaca acggtttatt ggctgggagt
    10741 gggtaattct ccgacgagaa ttttcacaca atgatgaaaa cttgtacaaa agaatcaagg
    10801 cgacaaagaa aacgaaaata cacaacattc tggtaactat gggagggagt gatcccaagg
    10861 gcatgactct gaaaaccatg gaagctttaa aggttataga tggtcagttt gaggtcacga
    10921 tcatacttgg tccaggattt gctcacagag acagtcttaa cgaaatactc ggcaactttc
    10981 ctcatataca caggatcttt gaaaatgtgc tgaatat
//
LOCUS       DAOOMC010000093        10923 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdus sp. isolate AMR_MDS_2685
            NODE_6787_length_10923_cov_6.649153, whole genome shotgun sequence.
ACCESSION   DAOOMC010000093 DAOOMC010000000
VERSION     DAOOMC010000093.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864760
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdus sp. (wastewater metagenome)
  ORGANISM  Syntrophorhabdus sp.
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae; Syntrophorhabdus.
REFERENCE   1  (bases 1 to 10923)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10923)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,729
            Genes (coding)                    :: 2,708
            CDSs (with protein)               :: 2,708
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 7 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10923
                     /organism="Syntrophorhabdus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6787_length_10923_cov_6.649153"
                     /isolate="AMR_MDS_2685"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2699756"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(257..3016)
                     /gene="ppdK"
                     /locus_tag="PK125_09860"
     CDS             complement(257..3016)
                     /gene="ppdK"
                     /locus_tag="PK125_09860"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016291309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050242 - pyruvate, phosphate dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HPB38447.1"
                     /translation="MKKYVYFFGGKKAEGGEKLRNLLGGKGAGLAEMINLGIPVPPGF
                     TITTEACVYFYQHKMAFPDGLKEEVQKNLKKVEKEMGAIFGDPKNPLLFSVRSGARVS
                     MPGMMDTVLNLGLNEKTVKGLGERTNNEWFAYDCYRRFVQMYGDVVLGLKPQTKEEHD
                     PFEDLIEKKKKDRKITLDTEMSVADLKDLVNSFKGLIKTRLGIVFPEDPLEQLWGAIG
                     AVFKSWNTERAIAYRELNNIPDTWGTAVNVQCMVFGNMGANCGTGVAFTRNPATGENQ
                     FYGEYLLNAQGEDVVAGIRTPLPINKKQKTDKSVKSLEEVMPGIYKELLKIIQILEGH
                     FKDMQDIEFTIQDGKLWMLQTRTGKRTAFSAFKIAVDMVKEKLIDKEEALMRVEPEQL
                     NQLLRPIFDPKEKEKTLKAGNLMTKGLNAGPGAATGKVVYNAEDAESWAAKGEKVILV
                     RIETSPEDIRGMNAAQGILTSRGGMTSHAALVARQMGKVCVAGCGDLDIDYKKKTISV
                     NGKTIREGEYISIDGTTGEVFSGEIKTIPSEILRVLVDKTLRPSQSQVYQDFALLMKW
                     ADETRKLGIRTNADQPDQAAIAIAFGAEGIGLCRTEHMFFESERIDVVREMIISDDRE
                     GRVKALKKLLPIQKKDFVAIFKVMKDLPTTIRTLDPPLHEFLPHTEKEIKDLAQKMGI
                     SAEKVSTKVESLHEANPMLGHRGCRLGIVFPEITEMQAQAIFEAACDVKKKGFNVKPE
                     IMIPLVGNVAELVLQKEVVDRVAQEVMKKTKVKIDYKVGTMIEIPRAAITADEIGTVA
                     EFFSFGTNDLTQTALGMSRDDAGKFLGYYVENGIYAFDPFQRIDEDGVGKLMKIGVDL
                     GRKARKNLKIGICGEHGGEPNSVEFCHKTGLNYVSCSPYRVTVAKLAAARAAIKEKKG
                     ERALD"
     gene            complement(3027..5099)
                     /gene="glyS"
                     /locus_tag="PK125_09865"
     CDS             complement(3027..5099)
                     /gene="glyS"
                     /locus_tag="PK125_09865"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: protein motif:HMM:TIGR00211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009345 - glycine-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HPB38448.1"
                     /translation="MKTLLLEIGVEEIPARFIEPAKEGLVRNLQESLNVARLPFNDIK
                     VSATPRRLTAFVHGLAEKQGETVIIKYGPPFNRAFDETGNPTKAAIGFAKSQGVEINE
                     LSKGQKDGVDFIIVERREKGESASTVLPGLLKDVIGKIPFQKKMRWGDQSFEFARPIQ
                     WLLILFENEQINVQVADVKSGRVTFCHRFLSQGPHTIKNPAEYVDTLRSNWVVIDEIE
                     RMSIIDKGIEKIENDVGGKALREEELIKEILYITEFPYPLCGSFEKSFLKIPKEVLVN
                     VMKSHQRYIPIQDNTQSLMPYFIFFANTKPIEDTIVIKGNEKVLKARLADAEFFFEED
                     KKTNLTELYERLSSIVFHVKLGTVKEKSDRLGSICRYLCSILNYSDSVLLEKAVKIVK
                     ADLLTHMVGEFPELQGTMGRIYAQVQGEHPDVAASIEEHYLPSSVDGALPQTQLGSII
                     AIADKIDSVVSFFSVGITPTGNLDPYALRRQTLGIIKIIIDRKLHIPLHGLFSAAYES
                     GKNIGKRSSFEDTKASLTDFVTARFKFSMIDEGHNQEFVESLLPVVGEDIYDGYLRLV
                     ALESQKSVEDFQKLLVGFKRAYNITKQISDDHTVDPVLFKEKEEDALFNAYESKKELF
                     FSAMRLRNYDDAISILISFKESIDRYFDKVFVMDKDENIKINRLALLKKIKDMFLAYA
                     DFSKIRIE"
     gene            complement(5174..6049)
                     /locus_tag="PK125_09870"
     CDS             complement(5174..6049)
                     /locus_tag="PK125_09870"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014896110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HPB38449.1"
                     /translation="MYFQDLILSLQKFWAEKGCIIQQPYDMEVGAGTFHPATFLRSLG
                     PEPWNTAYVQPSRRPADGRYGENPNRLQHYYQFQVIMKPSPKDIQDIYIESLRSFGID
                     PAYHDIRFVEDDWESPTLGAWGLGWEVWLDGMEITQFTYFQQCGGIDLSPITVEITYG
                     AERIAMYLQDVDNVYHVKWNENILYGDVFLEPEREFSIYNFEESDSEMLRELFNIFER
                     EGKRLIDGRGLVFPSYDYCLKCSHIFNLLDARGAISVTERANYIARVRNLAKICAELY
                     VKKRDELGFPLLKNN"
     gene            6159..7343
                     /locus_tag="PK125_09875"
     CDS             6159..7343
                     /locus_tag="PK125_09875"
                     /inference="COORDINATES: protein motif:HMM:NF024423.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HPB38450.1"
                     /translation="MVVKDFIIKVLDAASMQRWNDKIRPVDLRELDKQAHKMIIAYFL
                     AKFEESRDEFSWLEIIDGGLFEFLQRLVITDLKPQIFDRIKQDRKKYKELNEWVFKRL
                     EPIISPLGVEFCRKFRSYFATTDNTLNKRILSAAHFYATQWEFNIIERANPQGYEIRE
                     IKTHLQEKQERYYDLIGIQQLALYEKYRNFIDLCGQLRFQHRWGHITMVPHTSVLGHM
                     LTVAFLSYLFTVEIQGCTKRIVNNYLTGLFHDLPEALTRDIISPVKRSIEGLNELIIL
                     YEKEQMEKEVYGSLPPEWHNEMKMFTEDEFLNIVSVNAVTSRIDSDTISQKFNTDAFN
                     PRDGEIVKAADDLAALVEAYISIGNGIKSKDLFDSQKSIKERYRNRNIGGIDFGLIME
                     WF"
     gene            7410..8162
                     /locus_tag="PK125_09880"
     CDS             7410..8162
                     /locus_tag="PK125_09880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB38451.1"
                     /translation="MSKTSIKKTRTEKDKPGPLRDILDTVVVLSASEIPEKAIETVLT
                     GLSGRLGKRARCALLEGKDLNLRFWAGEHTCPIGGVKIRENSIVWDAVKKGIPINLTD
                     GHQSDHFEHTLGDPINVKSIIPLSYDDPLTKQQMKLGALIVDSGKEGVPISDEDFEYL
                     QVIGQLISAIVGRKALIEQLMQSCRRQEAILMEAAHNFRNDILIIGGFSRRITKLAKN
                     TEIAKIALDLQEEVRDLEKHFAEFERNINLES"
     gene            8629..9591
                     /locus_tag="PK125_09885"
     CDS             8629..9591
                     /locus_tag="PK125_09885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB38452.1"
                     /translation="MIENAYVCFFDVLGFTSSFISGTLSKRYDGLIEMMNSRRDPDVT
                     IFLMSDSIFCVSADFQKLKDTAKEFYTWGILNDFWLRGAITQGNVTKYREHIIIEQNR
                     FIIPFLGDGFLKAYTLQTTLNISGVVLDKIFFESDESNPGFIRGIDYIEYEEYLPKTG
                     YEGIEKLLLPKSDSLRQVVDSMYFAEMLESHAEDVDKYINTFCFYIQHLLERASTENL
                     IAFVEKMLKEFAFQTGRILIPTKIVTIFIAMIQGMFNRYRSSVGFYQCDPGQLGFLVN
                     QIVSVLKQQGYLSIFVDTMLEFDKSRHTSLYKEINNLSRLNVNR"
     gene            9703..10407
                     /locus_tag="PK125_09890"
     CDS             9703..10407
                     /locus_tag="PK125_09890"
                     /EC_number="2.3.1.286"
                     /inference="COORDINATES: protein motif:HMM:NF014228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sir2 family NAD-dependent protein deacetylase"
                     /protein_id="HPB38453.1"
                     /translation="MNKSVEERIESFAKMMFKSRHLVMFTGAGISTESGLPDFRGPDG
                     VWTRQDKGLPPPTTSKPWHLFEPNTGHYAIVNLQKAGKLKFLISQNVDNLHLKSGIKP
                     ELIAELHGNITKLKCPHCDMVYDTFWGFTECKCGSSLVPSTVGFGQSLPEHDLMLAFK
                     HSRKSDLFVVIGSSLVVTPAADMPKEALQHGAKLVIINEGDTPFDRYAHIRFRERIGD
                     ILPAAVNRLMVLMGLG"
BASE COUNT         3057 a         2439 c         2202 g         3225 t
ORIGIN      
        1 ttaagtgtaa aaaaagaccc ggttttaacc gggtctcaaa ggctcgcatt cttttgtttt
       61 aaacatctct cgtgtcttac tttttttatg ttttgccacg ccttatcggg aataatcgac
      121 tcaagagcaa tgtcttattc gatcatgcca gccatgacaa accgagccac tgttatgtct
      181 cgacgcgaac cctcttaata ttgttaatag cgtaagatca gggttctgtc aaggatagaa
      241 gaaacgggaa acatgattaa tccagtgccc tttcgccttt cttctctttg atggctgccc
      301 tggccgctgc aagctttgca acagtcactc tgtatggtga gcagctcaca taattcagac
      361 ctgtcttgtg gcaaaattct actgaattag gctcaccacc atgctcaccg cagataccga
      421 ttttcagatt tttacgtgcc tttctaccaa gatcaacgcc aattttcatt agtttgccta
      481 ccccgtcctc atcaattctc tggaagggat caaaagcata aatgccgttt tctacataat
      541 atccgagaaa tttgcctgca tcgtccctgc tcatccccag tgctgtctga gtcagatcat
      601 tcgttccaaa cgagaagaat tctgctactg ttccaatttc atccgctgtt atggctgccc
      661 tgggtatctc tatcattgtt ccgaccttgt aatcaatctt cacttttgtc tttttcataa
      721 cctcctgagc taccctgtct acaacttctt tctgcaacac aagttctgct acattgccaa
      781 ccagagggat cataatttca ggtttcacgt tgaaaccttt tttctttaca tcgcatgccg
      841 cttcaaatat tgcttgagct tgcatctcgg taatctcggg aaagacaatt ccaagcctgc
      901 atccacgatg accgagcatt gggtttgctt catggaggct ctccaccttt gttgacactt
      961 tctctgcgga tatgcccatc ttctgcgcca aatcttttat ctctttctct gtatggggta
     1021 gaaattcatg gagaggtgga tcgagcgttc tgatggttgt aggcaggtct ttcattacct
     1081 taaagattgc tacaaaatct tttttctgaa tgggaaggag ttttttcagt gcctttaccc
     1141 taccttccct gtcgtctgaa atgatcatct cccgaactac atcaattctt tcactctcaa
     1201 agaacatatg ctcggttcgg cacaggccaa ttccttctgc tccgaaggcg atagctatgg
     1261 ccgcctggtc aggctgatcg gcattggttc gaatgccgag ttttctcgtt tcatccgccc
     1321 acttcatgag aagcgcaaaa tcctgataaa cctgagattg tgatggcctg agtgttttat
     1381 ctacaagtac ccgtaagatt tcagatggaa tggttttgat ctcaccgctg aagacttcgc
     1441 ctgttgtacc atcaatggat atgtattcac cctccctgat ggttttgcca tttactgata
     1501 tggttttctt cttatagtca atgtccaggt caccgcatcc tgccacgcat actttgccca
     1561 tctggcgcgc cacaagggca gcatgacttg tcatgccacc gcgagatgtg aggataccct
     1621 gagctgcatt catgccacgt atgtcttccg gagatgtctc gatcctcacc aggataacct
     1681 tttcaccctt tgccgcccag ctttcagcgt cttcggcgtt ataaacaact tttcccgtag
     1741 cagcgccagg gcctgcatta agtccttttg tcataaggtt accggccttc agagtcttct
     1801 ctttttcctt agggtcaaaa attggacgca ataactggtt cagctgttct ggctcaacgc
     1861 gcatgagcgc ctcttccttg tcgatcagtt tttctttcac catatccaca gcaatcttaa
     1921 acgcagaaaa tgcggttctt ttccctgttc gtgtctgaag catccagagt ttaccgtcct
     1981 gaatggtaaa ctcaatatcc tgcatgtctt taaagtggcc ttccaggatt tgaataatct
     2041 tcaacaattc tttgtagata cccggcatta cctcttcaag ggattttacc gacttatctg
     2101 ttttttgctt cttattgatt ggcagaggtg ttctgatgcc agcaacaacg tcttcgccct
     2161 gagcattaag caaatattca ccatagaatt gattctctcc ggttgctggg tttctggtga
     2221 aggctacacc tgtcccgcag tttgccccca tattgccgaa gaccatacac tggacattca
     2281 ccgcagttcc ccatgtatca ggaatgttgt tcaattcacg gtaggcaatg gccctttcag
     2341 tattccacga tttaaaaact gcaccgatag ccccccacag ctgttccaga ggatcttcgg
     2401 gaaaaacaat gccaagacgg gttttgatca ggcctttaaa actgttaaca aggtctttaa
     2461 ggtctgcgac actcatttct gtgtcgagcg tgatcttgcg gtccttcttt ttcttttcaa
     2521 ttaagtcctc gaatggatcg tgctcttcct tggtttgtgg ctttaacccg agaacaacat
     2581 cgccgtacat ctgtacgaac cttctgtaac aatcataggc aaaccattcg ttgtttgttc
     2641 gttctcccaa acctttgact gttttttcat tgaggccgag attgagaacg gtatccatca
     2701 tgccgggcat actaacccgt gctccggatc tcactgagaa aagcaatgga tttttgggat
     2761 caccaaagat tgcgcccatc tctttttcaa ccttttttag gtttttttga acctcttcct
     2821 tgagcccgtc agggaaagcc atcttatgtt gataaaaata gacacatgcc tcggtagtaa
     2881 ttgtaaaacc cggaggaaca ggtatcccga gattaatcat ctcggctaag ccggcacctt
     2941 taccaccaag aagatttctt agtttttctc cgccttctgc tttcttgcca ccaaagaaat
     3001 atacgtactt tttcattccc tcctccctac tcaatgcgta tttttgaaaa atcggcatat
     3061 gcaagaaaca tatccttgat ctttttaagc agtgccagcc ggttgatctt gatattttca
     3121 tccttgtcca tgacaaaaac cttgtcaaaa tatctgtcga tactctcctt gaaacttatc
     3181 aggattgaaa tggcatcgtc atagtttctc aacctcatgg ctgaaaaaaa cagttctttc
     3241 ttcgattcat aggcattaaa aagtgcatct tcttctttct ccttgaagag caccgggtca
     3301 actgtgtgat cgtcagatat ctgtttcgtg atattatagg ccctcttaaa cccaaccagg
     3361 agtttttgaa aatcctccac tgacttctga gattccaggg ctacaagcct cagataacca
     3421 tcatagatgt cttctcccac aacaggaagg agactctcta caaattcctg gttatgacct
     3481 tcatcgatca tggaaaattt aaaccgcgct gtcacaaaat cagtaagtga agcctttgta
     3541 tcttcgaagc ttgaccgttt gccaatgttt tttccactct cgtaagcagc actgaaaagc
     3601 ccatgcagag gtatatgcaa ttttctgtca attataatct tgataattcc gagcgtctgt
     3661 cttctcaaag catatgggtc taggtttcct gttggtgtta tccccacaga gaaaaaggag
     3721 acaacactgt cgatcttatc cgcaatagcg ataattgatc cgagttgtgt ctgcggcaga
     3781 gctccgtcta cgctgcttgg cagataatgt tcttcaatcg aagccgcaac atctgggtgt
     3841 tccccctgga cctgggcata tattctgccc attgttccct ggagttcagg aaattctccc
     3901 accatgtgtg taagcagatc tgctttgaca atcttgactg ccttctccaa caatacactg
     3961 tcactgtaat tgagaataga acataggtac cgacatattg aacccaatct gtcacttttt
     4021 tcctttaccg tcccaagttt aacatgaaat acaatcgaag acaggcgctc atataactcg
     4081 gtaaggtttg tttttttgtc ctcctcaaag aaaaactcag catcggcaag ccttgcctta
     4141 agaacctttt cgttgccttt gataacgatc gtgtcttcaa tcggtttggt attggcaaaa
     4201 aatatgaaat aaggcatgag tgattgcgta ttatcctgaa tagggatata tcgctggtga
     4261 ctcttcatta cattcaccag tacctctttc ggtattttca gaaaagattt ttcaaaactc
     4321 ccgcacaggg gatagggaaa ctcggtgata taaaggattt cctttatgag ttcttcttct
     4381 ctgagtgcct tgcccccgac atcattctca attttttcta tgcccttatc gataatggac
     4441 atacgttcaa tctcgtcgat aacaacccaa ttcgaacgca atgtatcgac gtattctgcc
     4501 ggattcttta ttgtgtgcgg tccctgtgaa agaaatctat ggcagaaggt aactcttcca
     4561 cttttgacat ctgcaacctg aacattgatc tgctcgtttt caaataaaat aaggagccat
     4621 tgaattggac gggcgaactc aaaagattgg tcaccccagc gcattttctt ctggaaggga
     4681 attttaccaa taacatcctt caacaatccc gggagcacag tagatgcaga ttcacccttt
     4741 tctcgcctct ctacaataat aaaatcgact ccatcttttt ggcctttact gagttcatta
     4801 atctctactc cttgtgattt agcaaagcca atagcagcct ttgtcggatt accggtttca
     4861 tcaaacgctc tattaaaggg aggaccatat ttgattataa ctgtttcacc ctgtttttct
     4921 gctagtccgt gcacaaatgc cgtcaacctt ctgggtgtag cagatacttt tatgtcgttg
     4981 aaaggcaacc ttgcaacgtt cagactttct tggagatttc tcacaaggcc ttcttttgct
     5041 ggttcaataa aacgtgcggg gatctcttcc acgccaattt caagcagtaa tgttttcacc
     5101 ttaaatttat cctgttcttt gagataaagt tggaaaccct gtctctcaac ctggaatctg
     5161 caacctgaaa ctattaatta ttttttaaga gaggaaaacc cagttcgtct ctttttttca
     5221 cgtataattc tgcacatatc tttgcaaggt tccgtacccg cgcaatgtaa tttgccctct
     5281 ctgtcacgct gatggccccc ctagcgtcaa ggaggttaaa aatatgcgag cacttcaggc
     5341 aataatcgta cgaagggaat acaagccctc ttccatcaat aagccttttg ccttcccgtt
     5401 caaaaatatt gaagagttcg cgtaacattt cagagtcgga ctcctcgaag ttatatatgg
     5461 aaaactcccg ttcaggttca agaaacacat cgccgtacag aatgttttca ttccacttca
     5521 catgatacac attgtctaca tcctgaaggt acatggcaat tctctcagca ccatatgtta
     5581 tctcaacagt aataggagaa agatcaatgc ccccacactg ctgaaaatag gtaaactgcg
     5641 taatctccat accatcaagc cagacttccc agcccaatcc ccacgcacca agggtgggtg
     5701 attcccagtc atcctcaaca aatcggatat catgatacgc agggtctata ccaaagcttc
     5761 tgagactttc aatgtaaata tcttgaatat ctttggggga cggtttcatt ataacctgaa
     5821 actggtagta atgctgaaga cgatttggat tctcgccata tctgccatca gccggtcttc
     5881 tagacggttg cacatatgct gtattccacg gttctggccc gagggagcga aggaaagttg
     5941 caggatggaa ggtgccggca cctacttcca tatcataggg ctgttggatg atgcatcctt
     6001 tctcagccca aaacttttga agtgacaaaa tgaggtcttg aaaatacata acctttcgcg
     6061 caaaatgtat atcataaaag attagttttt tgccattatt ttgtgaatat attgccaaat
     6121 ttacttggtg ttgatattct ttttctatct ggatggatat ggtcgtcaaa gatttcataa
     6181 taaaagtact cgatgcagca agcatgcaaa gatggaatga caagattcgc ccggttgacc
     6241 tgagagaact tgataaacag gctcacaaga tgatcatagc ttactttctc gccaaattcg
     6301 aggaatcaag ggatgagttt agctggctgg aaataattga tggagggctc ttcgagttcc
     6361 ttcaacggct tgttattacg gacctaaaac ctcaaatctt tgatcgcata aaacaggatc
     6421 gaaaaaaata taaagaatta aatgaatggg ttttcaaacg tcttgaacct ataatctcac
     6481 ctttaggagt agagttctgc cgcaagttca ggtcatattt tgcgactact gataatactt
     6541 taaacaagcg aatattgagt gcagcccatt tctatgcaac acagtgggag ttcaacatca
     6601 tcgaacgggc aaatccacaa ggctatgaga tacgggagat caaaacacac cttcaggaga
     6661 agcaggaacg ttactatgac ctgataggca ttcagcagct tgccctttac gagaaatatc
     6721 gcaatttcat cgatctctgc ggtcagttga gattccagca ccgatggggc cacatcacca
     6781 tggttccaca cacttcagtc cttggacaca tgctcactgt tgcatttctc tcctatctct
     6841 ttactgtcga gatacaaggg tgcacaaaaa ggatcgttaa caattatctc acaggcttat
     6901 ttcacgatct gcccgaggca ctcaccagag atatcatttc tcctgttaaa agatccatag
     6961 aagggctgaa tgagctcata atactttatg agaaagaaca gatggaaaaa gaagtttacg
     7021 gttcactacc acctgagtgg cacaacgaga tgaagatgtt tacggaggat gaattcctga
     7081 acatcgtgag tgtaaacgct gtaacatccc gcattgattc agatacgatc agccagaaat
     7141 ttaacactga tgcgtttaat ccccgtgatg gtgaaatagt taaagctgct gatgaccttg
     7201 ctgcccttgt tgaggcttat atttccatcg gtaacggtat caaatcaaaa gacttatttg
     7261 attctcaaaa atctattaag gaaaggtaca gaaatagaaa tatcggaggt attgattttg
     7321 gcctgatcat ggaatggttt taattctctt gccttgatca tagcattgaa gaaacgttat
     7381 catagtatag tattgagagg agctatccaa tgtccaagac ttcaatcaaa aaaacaagaa
     7441 cagagaagga taaaccagga cccttgcgcg acattctcga tactgtcgtt gttcttagtg
     7501 cgagtgaaat ccctgaaaaa gccatagaga ccgtccttac aggtctcagt ggaagattag
     7561 gtaaaagggc tcgatgtgct ctccttgaag ggaaggattt gaatcttcgc ttctgggcag
     7621 gagaacatac ctgtcccata ggcggtgtta agataaggga aaatagtatc gtatgggatg
     7681 cggtaaaaaa aggaattccc atcaacctta ccgatggaca tcaatcagac cattttgaac
     7741 atacattagg tgatcctata aatgttaaat ctatcatccc tttaagctat gatgatcccc
     7801 tgactaaaca gcaaatgaaa ttaggtgcgc taattgttga ttcaggaaaa gaaggcgttc
     7861 caatttctga cgaagatttc gagtatctcc aggtgattgg tcagttaatc agcgcaattg
     7921 tcggaagaaa agcacttatc gaacagctta tgcagtcatg ccgtcggcaa gaagctatcc
     7981 ttatggaggc tgcccataat tttagaaatg acattttaat cataggagga ttttcacgac
     8041 gaataaccaa attggcaaag aatactgaaa ttgctaaaat tgccctggat ttacaggaag
     8101 aggtaagaga ccttgaaaaa catttcgctg agtttgaaag gaatatcaac ctggaatcct
     8161 gatacgctaa catgtcgaca aaaatttatg aatcggttac aattacaaag aatacattgt
     8221 tcgctgcaat ctaatttcag tgacgaaggt tggtcaaata gtaacggtat agggattatt
     8281 ccttttttgc ttttattgac atttattctc attatgcctc gggcacaagg agcaggatag
     8341 ctttcagcag gcgcagtatt aggaggtaat aaattctttt atattaatgg gacgctaaca
     8401 gaatttcaaa acagtataat aagatttttg ggaaaagatt gaatattctg gcaagcaaag
     8461 gaaaaaatat caaatggaga attggtaatc gatatgattt tacccataac ctctaaatgg
     8521 cagccttctg aaggttggtc gcccataatt cctgattcga tgatggaaag tttctataag
     8581 gcgctttgtt ctcaacagga aaaaaagtaa ctaaaaggtt cctctaatat gattgaaaat
     8641 gcttacgtct gttttttcga tgttttaggg tttacttcca gcttcatctc aggaacgctc
     8701 tcgaaaagat acgacgggtt gattgagatg atgaactcac gcagagatcc agatgtaacg
     8761 atcttcctca tgtctgacag catcttttgt gtatctgctg atttccagaa actaaaggat
     8821 acggccaagg agttctatac atggggcata ctcaatgact tttggctcag aggggcaata
     8881 actcagggaa atgtgacaaa atacagggaa catattatca tcgaacagaa caggtttatc
     8941 attccttttc tcggcgatgg atttctgaag gcctataccc tccagaccac actcaacatt
     9001 tctggtgtgg ttttggataa aatattcttc gaatcagatg aatcgaatcc gggctttata
     9061 agaggaatag actatattga atatgaggag tatctgccaa aaaccgggta tgagggtata
     9121 gagaagcttc ttcttcctaa atctgattct ctacgtcagg tggtggatag catgtatttt
     9181 gctgagatgc tcgaatctca cgctgaagat gtggacaagt atataaatac tttctgtttc
     9241 tacatacagc atcttttgga gcgggcaagc actgagaatc ttattgcttt tgtagaaaag
     9301 atgttgaaag agttcgcgtt ccagactggc aggattctaa tccctaccaa gattgtaact
     9361 atattcatag caatgattca gggaatgttt aaccgatatc ggtcatctgt cgggttttat
     9421 caatgtgacc cgggacagct tgggtttctc gtaaatcaaa ttgtgtctgt tcttaagcag
     9481 caaggttatc tttccatttt tgtcgacacc atgcttgagt tcgacaaaag cagacatact
     9541 tctttgtaca aggaaataaa caatctcagt cgcctaaatg taaatagata ggtagaccag
     9601 catacttctg ctatccaatt taccgcctaa cagtcctacc agattgtggt cacgtcttct
     9661 tgctattcac tcttttcaga catatagtct taggagtatg agatgaataa gagtgttgag
     9721 gaaagaatcg aatcatttgc aaaaatgatg ttcaaatcgc gccatcttgt tatgtttaca
     9781 ggtgcgggta ttagcacgga atcaggactc cctgacttca gggggcctga tggagtatgg
     9841 actcgtcagg ataaagggct gccgccgcct acgacaagca agccatggca cttgtttgag
     9901 ccaaacacag gccattatgc tattgttaat ctacagaaag caggaaaact caaattctta
     9961 atctcccaaa atgtggacaa tcttcacctg aagtcaggaa taaagcccga gctcattgca
    10021 gaactccatg gtaatattac aaaactaaaa tgtccgcatt gtgatatggt atacgacacc
    10081 ttctgggggt ttactgaatg caaatgtggc agttcactcg tgccaagtac tgtgggcttt
    10141 ggccaatccc ttccggaaca tgatcttatg ctcgccttta aacattcacg aaaaagcgat
    10201 ctttttgtgg taattggctc aagtctcgtg gtaacaccag cggcagacat gccaaaggaa
    10261 gcgctccagc atggagcgaa actcgttata atcaacgaag gagatacgcc atttgaccgc
    10321 tacgcccata ttcgatttcg tgaaagaatt ggggatatct tgcccgctgc tgtaaaccgg
    10381 ttgatggttc tcatgggctt gggataatta aaaaaaatcc ggtaaagagg gcttgtgcca
    10441 ggttcgttca aactggtagg attgactaaa catatctatg ccagagacac aaaagagaac
    10501 gctatatcag gccatctcga ttttattcag gtatttccac caatgttcac acacgacctt
    10561 agagcacgga tggaaagtga tccccagtta caagcatgca taaacaattt tacaccaccc
    10621 cgctatcaaa ctacagctat cctaagatag atataccccc gtttcaaatg caccaatgaa
    10681 ggggtgcaat gagacccgca ggattgatga atcatttgga acagcatgag ctcaaggtat
    10741 gagaatacag gcatgaaagg atcaggaaca gttccagagg tggcaagcca tcgttcttac
    10801 ccgtaaaaga tagaggacag aggcttaaca gagaagacac acgactggtg aggatcatat
    10861 gctgaattac tcttttcttt attcttctct ggcaataatg taccgaaata aagatataaa
    10921 ggg
//
LOCUS       DAOOMC010000094        10828 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdus sp. isolate AMR_MDS_2685
            NODE_6878_length_10828_cov_5.509422, whole genome shotgun sequence.
ACCESSION   DAOOMC010000094 DAOOMC010000000
VERSION     DAOOMC010000094.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864760
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdus sp. (wastewater metagenome)
  ORGANISM  Syntrophorhabdus sp.
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae; Syntrophorhabdus.
REFERENCE   1  (bases 1 to 10828)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10828)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,729
            Genes (coding)                    :: 2,708
            CDSs (with protein)               :: 2,708
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 7 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10828
                     /organism="Syntrophorhabdus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6878_length_10828_cov_5.509422"
                     /isolate="AMR_MDS_2685"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2699756"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..489
                     /locus_tag="PK125_09895"
     CDS             <1..489
                     /locus_tag="PK125_09895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB38454.1"
                     /translation="IPIIPERKGNKAGAFGIGGNAFVGQNLGWYGGIGGYTVDPGIPG
                     LVEPRISYPHQWGGWFQLDYFFTNAFSFHGWYSHAKNNFSRHAAFFGWLTPPMSIRQN
                     QQYIANLSYDVNPAMRFGLEYAHIRTKYANYQWSTAAVPEIVAGKKGTLNTIRIGAWY
                     FF"
     gene            614..701
                     /locus_tag="PK125_09900"
     tRNA            614..701
                     /locus_tag="PK125_09900"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:648..650,aa:Leu,seq:caa)
     gene            complement(1127..1666)
                     /locus_tag="PK125_09905"
     CDS             complement(1127..1666)
                     /locus_tag="PK125_09905"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HPB38455.1"
                     /translation="MNIITIDATLLAKRNPLLKGVNPALCDTLCRFGSKDMRHTNNKI
                     QTPLLTVDIIIRFQDGIVLIERKNPPPGWALPGGFVDVGESLEKAAIREAKEETSLNV
                     TLVEQFHAYSKPGRDPRFHTVTMVFIADGTGTLKGRDDARKAQVFTEATLPTPVAFDH
                     GNILYDYFSYIKTGLRPQT"
     gene            1669..2406
                     /locus_tag="PK125_09910"
     CDS             1669..2406
                     /locus_tag="PK125_09910"
                     /inference="COORDINATES: protein motif:HMM:NF016777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HPB38456.1"
                     /translation="MEFDLIIDSHSHWGPSLSMGTEVTTAELQRQLKASGISHVVLIP
                     FPSTAIANNEINIKLLEETKQIQSFIPYHYIRENYDTDGFNPIPEAYFGGKWHWMRGW
                     QDTASNYEVLDDPVLPGLIEKLRKINKPIVFEEDFEFTVKFVNMAEDVTLIIPHLGLL
                     GGNPYDFLRAFKDNESIYFDTALASRDQIKKFVETIGPERVIFGSDVPFGSMASELDK
                     VLSLHIPREDKELLLYKNIINLAKLDI"
     gene            3454..4146
                     /locus_tag="PK125_09915"
     CDS             3454..4146
                     /locus_tag="PK125_09915"
                     /inference="COORDINATES: protein motif:HMM:NF019349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HPB38457.1"
                     /translation="MDLTPVKKAKLIDIVTNKIRDEILSGNYVAGDKLPPEREFVDQL
                     QVSRIVVREALRRLEATGLLTVKRGSGMFVASHNSTALHDAFSLILKIQKVDALEVIR
                     AGLAIEPAMTELAAKNRTSEHITCLKANIKRSEELIKGGRTSNNNNREFHRIVAAATQ
                     NKVLRLAMEAILHVLNVVHAPHGVPTCGDDALIWHRKIMEAIEDSKPQEAAKLMHDHI
                     AEVQKTVEEVIA"
     gene            4695..5006
                     /locus_tag="PK125_09920"
     CDS             4695..5006
                     /locus_tag="PK125_09920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB38458.1"
                     /translation="MAKEIILYNLKTTVTDEEYAEYCKTKKGPFLNSLPSCKKFTLVK
                     IVGSKKGEIPYKYVGIVDATSGEEWMKDASSPAFQDFLKEWMTKVEDFHILMGEEVFG
                     D"
     gene            5038..6246
                     /locus_tag="PK125_09925"
     CDS             5038..6246
                     /locus_tag="PK125_09925"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HPB38459.1"
                     /translation="MYLKDLLTKEQHDIRQTIKKFVDKEIMPIREKLEEDYSLVESVH
                     KKLVDMGIQRAGYPEEYGGGGSHSATTLGIVCEELARGDAGISLSTGINGGIILQPAM
                     MAKNKAILDKFVPAFCENKVNYACLSMTDSAGGADSENPLLKGKYIATKAELDGNEWV
                     INGNKSWPTHAGLASVYLTVCTTDPKLADDGIAIIYVPGDAKGLSFGKPETKMGYRTS
                     INGSVFYDNVRVPKEFRLAGPGHDAHFYYAVMGGAQWHSSTMSLGIAQAAFDIALAYT
                     GERMSGSRPVREWSLAAGVLADMAIRLETTRALVYNYAWMMDHPEQYGPPFTPEMVSR
                     AGIVRIYAADAAVWVANKAMELLGSNGLSPEYHLEKYLRDSKITQLWLGGQQVSRYRV
                     VRGYYDYTVG"
     gene            6563..7654
                     /locus_tag="PK125_09930"
     CDS             6563..7654
                     /locus_tag="PK125_09930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB38460.1"
                     /translation="MEKQGGLKQPALGIAGTVVCVFIAFGIITWFKPETFIPWAGELA
                     MCLIPTMIIMSLVWQNNYPAPAPSLVQPLKGVYLLFFNMLIGAIIAGYSIKTVGAFVT
                     PPTPPLIFFTIMTVIMTCWFVIVWGCWPAAAIKGNHPAFVGFGTLIVSYAVAYILWKT
                     CFNFDFMKGAPFYDAVAPPSGAFNAFYSLGFFLTALAVMLALVELDFWPLSSISPKAP
                     AFGKQPVWGIVVSVVVILISLLIQWIFVNTMGMDIFAYTLRVPICIIFGEFIMLLLFQ
                     TAPVQAMKQPGKGFVLIILNIILAVVMYYIYYWFQTLVAGILPAGPPGYVQELWIATA
                     LLGFTFPVITAYTGFFNFWPLTESKASKE"
     gene            complement(8168..9433)
                     /locus_tag="PK125_09935"
     CDS             complement(8168..9433)
                     /locus_tag="PK125_09935"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HPB38461.1"
                     /translation="MNTTTIFPFRPPRNCSLFIKFVQYILEPIMLPLIPKISRIEINP
                     INLSLLKSFKGKRVILISNHAEMTEPYVIFALSRMVGEEFNYLTAREVFEDYYPAGRL
                     LQALGCYSVIRGAPDRNSLRATSEILVKGKNWLVIFPEGVSTGASDVLMPFQPGIGQF
                     AFRAYDELTKKGEKPDIFFVPIAIKTFYIHNMDLPIDRALRHLETKLLPGTDQGKKDY
                     QERLVALGEAMLVSKEHEYGVSPQPGTSLSQRISTMKEVIIAATAKEIGVEDHPEHIL
                     PDRLRDLINTLDQYIHRQNQAKDTEESAKALRERAKMLRHSLDTATNFMALDHQYLEL
                     PMTTERFLDIVGLLERETFGKRRFWGQRKAVIKVGSPVNLNDFASQYRASKKDAFLAI
                     SRALEAKVRNMLQELSQRSELLQQLKKDG"
     gene            complement(9445..10719)
                     /locus_tag="PK125_09940"
     CDS             complement(9445..10719)
                     /locus_tag="PK125_09940"
                     /inference="COORDINATES: protein motif:HMM:NF023128.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPB38462.1"
                     /translation="MKHAGATKQGYETKTVISWAFYDWANSAYATTVMAGFFPVFFKQ
                     YWSAGTDVHVSTFNLGTANSLASVIVALLAPVLGSIADQWGNKKRFLLFFAVMGMVMT
                     GSLGFVAKGEWLVAIFLYAFANIGFTGGNVFYDSLMVCIVEKKKMDFVSALGYSLGYL
                     GGGILFAINVVMALKPHLFGFPSAVEAIRISFFSVAVWWALFSVPIFLFVREPKVRTA
                     QSVTDAARSGMKQLFSTFREIRKLKHIFLFLVGYWCYIDGVDTTVIMAVDYGLSLGFD
                     QTVLIKALLITQFIGFPAAILFGKIGEKLGTRIGIFIGITVYVAVAIWGYYMNDPAEF
                     YVLAIAVGLVQGGVQSLSRSLYGKMIPSENAGEFFGFYNMLGKFAAVLGPFLMGITSL
                     LTGNARCSIFAIIFLFVTGAFMLAFVKENSPR"
BASE COUNT         3134 a         2251 c         2478 g         2965 t
ORIGIN      
        1 atccccatta tccccgagag aaagggtaac aaggcaggcg cttttggtat cggcggtaac
       61 gcctttgtgg gccagaacct tggatggtac ggtggtatcg gaggatacac ggtggatcct
      121 ggtatacccg gtttggttga accgaggatt tcttatcccc atcagtgggg cggatggttc
      181 cagcttgact atttctttac caacgctttt tctttccatg gctggtactc ccatgccaag
      241 aacaacttca gcagacatgc cgcgtttttc ggctggctta cacctcctat gtccattagg
      301 cagaaccagc agtatatcgc caacctttca tacgatgtga accctgccat gagatttggt
      361 cttgaatatg ctcacatccg tacgaaatac gcaaactacc agtggagcac agcagcagtc
      421 ccagagatag tagcgggcaa gaagggaacg ttgaacacaa tcagaatcgg tgcctggtac
      481 ttcttctaag aaaagacaga cttcagtatc agagggaggc tccggcctcc cttttttatt
      541 acgtgcaagc attcctgttt gttgcttttc ttgacagaaa gattaacgtt ctgctatgct
      601 ttcatgatcg atggccggag tggtggaagt ggaagacgcg ccggactcaa aatccggtgg
      661 tactcggtac tgtgcgggtt caagccccgc ctccggcacc aaaaacgccg taaccccgga
      721 aaagggtaat cagttatcct taagaggcga cagcacaaaa ttcttcttaa atatagattg
      781 agcgcggtat gtcttctttt accagtcaga cgctctagta tcctgtaaag gttaaaatca
      841 aattatttcc ataaggatta cccgggcctg gaaagagtta tcctgtaagg catggtgtat
      901 ttgattctcc gagtttttat cgctttgtca attctctttc caccctcatt ctgagccata
      961 tgcatctttt atcggggcct gttgtgcgtc tgcgccggag aatcggtaga cagggtatca
     1021 cttgcagtcg tgaccttata gaatatgtgt tgatgaacat acagagattt tccagctaat
     1081 tcgaggttaa acagattaat ccctttcacc cggtcctttg agatgattat gtctgtggtc
     1141 gcaaccctgt cttaatatag ctgaagtaat cataaaggat attgccatgg tcaaaggcga
     1201 ctggagttgg aagggttgct tcagtaaaaa cctgtgcttt gcgtgcatcg tcgcgtcctt
     1261 tgagtgttcc ggttccgtca gctatgaata ccatggtcac agtgtgaaaa cgtggatcac
     1321 ggcctggttt tgaataggcg tgaaattgtt ccaccagtgt gacattgaga gatgtctcct
     1381 cttttgcttc tctaattgca gctttttcca gagattcgcc aacatctaca aacccgcccg
     1441 gcagagccca tcctggagga gggttttttc gttcaatgag cacaatgccg tcctggaagc
     1501 ggatgatgat gtcaaccgtt agcaatgggg tctgtatttt gttgttggta tgtcgcatat
     1561 ctttgctgcc aaacctgcaa agtgtatcac atagggcagg gttgacacct ttcaaaagag
     1621 gatttctttt tgctaaaagg gtagcatcta tggtaataat gttcaaccat ggaatttgat
     1681 ctcatcatag acagccattc tcactggggg ccgtctttat caatgggcac tgaagtaaca
     1741 actgctgaat tgcagagaca actcaaggca tcaggcattt cccatgtagt gcttatccct
     1801 ttcccttcta ccgctattgc gaataacgag atcaatataa agctccttga agagacgaag
     1861 caaatccaaa gctttatccc ttaccactat atacgggaaa attatgatac agacggattc
     1921 aatcctatcc cagaggcata ctttggtggt aaatggcact ggatgagagg atggcaggat
     1981 actgcctcaa attatgaggt actcgatgat cctgtgcttc ccggactgat cgaaaagtta
     2041 cggaagatta ataaacccat tgtatttgaa gaagactttg agtttactgt aaagtttgtc
     2101 aatatggcgg aagacgttac cctaataatt cctcaccttg gcctccttgg tggtaatcct
     2161 tatgattttc tgcgtgcgtt caaagataat gaatccatat attttgacac tgccctggca
     2221 tcgagggatc agatcaaaaa atttgtagaa accattggcc ctgagcgggt catattcggt
     2281 tctgacgtac ccttcggttc tatggcgagt gaactggaca aagtcctttc cctgcatatt
     2341 ccacgagagg acaaggaact gctactctac aagaacatca taaacctggc aaagctggat
     2401 atttaaaaag tagcatatcg tatgttgaaa gcaccctctg attcgtgaag cagcaggtct
     2461 gtcatagtca gaggatttca cagtgtatca atttcaaatt atcctttttc ttttgtgtaa
     2521 cctctgattt ctaacacggg gcactaccct ggaggattgc cggaagggaa ttaaattctg
     2581 accgtgtaaa acaaacaata aacattgttt tgtggtatct cttttttatt aaagtctggt
     2641 atcggcatat atatattcac caaaaaggga aaattgtgga tgtattggaa gagatagaag
     2701 aaacacggag tgtcgaagaa gttgtgggtc acaatcggtt agatttgaag ataaataaaa
     2761 cctagtgccc taacatgatt agtgacatcc tagtgtattg cacgggagga tatctgttgg
     2821 tgagaagcgg actgaacaac tcccagaagt ccatacagta aatatccttg gattagcagc
     2881 catagtttta ttcacggaag gaaccctttt tcagaaacaa taaagcttgc agaagaactg
     2941 aatatcctaa tattgacaaa caaaatatat cctctttcaa accgttataa gtttacactc
     3001 ttaaggattg ctggcagcat agaaaaggtt ggagagaata agccctagga acaggaattt
     3061 gagtcactta ccctgtgatg cgtggtgggg aatgtaaaat gcatgtcaca gcatttcatg
     3121 gtttgtagca caaaaataag ttcagcttcc ttcgtaccta aaatttcata tacaaagatc
     3181 ttatgtgaaa tcaaacactg tactgtttta ttgggtgtta cgagattcac aggactaatt
     3241 tgcaaagaaa aacttagaaa caatataaat aatctgtctc cttatgtaca atcaaggaca
     3301 gagtgccatt cgaaattaaa aacggtttag gtagtatggc acacaatgtt gtgaatctga
     3361 aagatataat atataataat tgatacaacg acgacgctaa aaacaataga ttttagaaca
     3421 aagcgcacaa acctataaga ggaatttgcc tacatggatt taacaccggt taagaaagcc
     3481 aagctcatcg atattgtaac gaataagatc agggatgaaa ttcttagtgg aaattatgtg
     3541 gctggcgata aactccctcc cgaaagggag tttgtggatc agcttcaggt cagtagaata
     3601 gttgttcgag aagcattacg aaggctcgag gctacaggcc tattaacggt gaaacgtggt
     3661 tcaggtatgt ttgttgctag tcataattcc actgcactac atgatgcctt ttccctgatt
     3721 ctgaaaattc agaaagtaga tgcattggag gtcattagag caggtcttgc catagagcct
     3781 gccatgacag aattagcagc gaagaacaga acatctgaac atattacttg cttgaaggca
     3841 aacatcaaga ggtctgaaga gctaatcaag ggaggccgga catcaaataa taataataga
     3901 gaatttcata gaatagttgc agcagcgacc caaaataaag tacttaggct tgccatggaa
     3961 gcaatactac atgtcttgaa cgttgtgcat gcccctcacg gtgtcccaac ctgtggagat
     4021 gatgcgctta tctggcatag aaaaatcatg gaggcaatcg aggatagtaa acctcaagaa
     4081 gctgctaaac tgatgcacga ccatattgcc gaagttcaaa aaaccgttga ggaagtgatt
     4141 gcatagggtt ttaatacagt ctatagaagt tcacaattgc aatccctttg caaatcaatc
     4201 cattgttttt ttcgattaaa gccttccaca gggttaacag tgaaggtaga ttcgttaagt
     4261 ggcaaaagtt ttcccctatt gcaatgtttg agaatctcac atacaaagct gtaaagctaa
     4321 atattgtatt cagctagatt caagtttgcc tgcaatgaaa aaaatcttga caatgttcca
     4381 ccaaaaagct aatttaacag ttatcagata tatgatatct aatatcattt atcttgatta
     4441 aataaagcta aaataggtcg tatagatgtt cagtgggtaa tgaggccttc tctgtccgat
     4501 ggaggacaag aggcctcgtt tatttcggtt ggggaaatcg agcttcgaat gaaatttatc
     4561 tggtgtgttt gggagttccg tgagggccga aaggaacaat gccaatcaaa gaaagggggt
     4621 gaatcataac atactatgtc atattgcacc aaaggtaaca agaagacaaa acgaatgatg
     4681 aaagggagga agtcatggct aaagaaatta ttttatacaa cttgaaaaca acggttacag
     4741 atgaggaata tgcggaatat tgtaaaacaa aaaaaggtcc gttcttaaat agtctgccat
     4801 catgcaaaaa atttacactc gtaaaaatag tagggtcaaa aaagggcgag ataccctaca
     4861 agtatgtggg cattgttgat gccacaagtg gtgaagaatg gatgaaggat gcatcatccc
     4921 ctgcatttca ggattttctc aaagagtgga tgacaaaagt ggaagacttt cacattttga
     4981 tgggtgaaga agtctttgga gattgatgta ctccagaata taacataggg ggttaatatg
     5041 tacttaaagg atttgttgac aaaggaacaa cacgacattc gtcaaacaat caagaagttt
     5101 gtggacaagg agatcatgcc aattagagaa aaacttgagg aggattatag tcttgttgag
     5161 tctgtacaca agaaactggt tgacatgggg attcaaaggg cagggtatcc ggaagagtat
     5221 ggcggcggag gcagtcattc agcaaccact ctcggcattg tatgcgagga attagcgagg
     5281 ggcgatgcag ggataagcct aagcacaggt attaacgggg gaataatcct ccaaccagct
     5341 atgatggcaa agaacaaggc aatccttgac aagttcgtac ctgccttctg tgagaacaag
     5401 gtaaactacg catgtttgtc catgactgat tctgcaggtg gcgctgattc ggaaaacccg
     5461 cttttgaaag gaaaatatat tgcaactaag gctgaacttg atggcaacga atgggttatc
     5521 aacggaaaca agtcatggcc cacccatgcg ggtctcgcaa gtgtttatct gactgtatgc
     5581 accactgatc ctaagttagc cgatgacggt atcgcaatca tctatgttcc cggagacgct
     5641 aaaggtcttt cctttggcaa accagaaaca aagatgggtt atagaacatc aatcaacgga
     5701 agtgttttct atgataacgt gagggttccg aaggaattcc ggctagccgg acctggacat
     5761 gacgctcatt tctattatgc cgtcatgggg ggagcccagt ggcactcttc gacaatgtct
     5821 ctcggcattg ctcaagcagc tttcgacatc gccctcgcat atacagggga gcggatgagt
     5881 ggctcaaggc ctgttaggga atggagtttg gcagcaggag ttctcgcaga catggcaatc
     5941 cggcttgaga caaccagggc actcgtttac aactacgcat ggatgatgga ccatcctgaa
     6001 cagtatggtc ccccctttac tcctgagatg gtatcaaggg ctggcatagt gagaatatat
     6061 gcagctgatg cagctgtgtg ggttgcaaat aaggccatgg aactactcgg gtctaacggt
     6121 ttatcaccag aatatcatct cgaaaaatac cttcgcgact caaaaataac ccagttgtgg
     6181 cttggagggc agcaagtctc gcgttaccgg gtggttcgtg ggtattacga ctataccgtg
     6241 gggtagcata taagtaaagg gctaagcata tgagacttgg cgcagagatt tttctgaaac
     6301 tggtcgatta cagaaatgca tcacggcgaa tgaggaacct cctttttcta caagaacaag
     6361 aggaggttcc tcctctttgt gtgacaaaga ataacagcta tcgaaaaacc atagacaacc
     6421 aactatgcgt gaaataaatg atagagcagg cagattgacg ctaggaatac gtaattaata
     6481 gcataaggac tgtgtataga ctgaacaaca tttcataaaa agtatacagc cacaaaaaat
     6541 aaatttaagg gaggggtatt ggatggagaa gcaaggaggg ttgaaacaac ctgcattagg
     6601 aatcgcagga actgtcgtat gtgttttcat tgctttcgga atcataactt ggtttaaacc
     6661 agaaacgttt atcccgtggg caggcgagtt ggccatgtgt ctcataccaa ccatgattat
     6721 catgagtctt gtttggcaaa acaactatcc tgcaccagca ccaagtttag tgcaacccct
     6781 gaaaggagta taccttcttt tcttcaacat gctcatcggt gctattattg ccggatattc
     6841 tattaaaact gttggggctt ttgtcacacc ccccactccg ccactaatat tctttacaat
     6901 tatgaccgta attatgacgt gctggtttgt tattgtgtgg ggatgctggc ctgctgctgc
     6961 tatcaaaggc aaccaccctg cctttgtagg ttttggaacg ctcattgtct catatgcggt
     7021 agcctacatt ctctggaaaa catgtttcaa ttttgatttc atgaagggtg ctccttttta
     7081 tgatgctgtg gcacctccaa gcggagcttt caacgccttc tacagcttag gcttcttcct
     7141 tacagcccta gctgtcatgc tggcgttggt agaacttgac ttctggccct taagctcaat
     7201 ctcgccgaag gcgcccgcat tcggtaagca acctgtctgg ggtattgttg tcagcgtagt
     7261 cgtaatactc atatctctgc taatacagtg gatttttgta aatactatgg ggatggatat
     7321 ttttgcatac acactgaggg ttcctatctg tattatcttt ggtgagttta ttatgcttct
     7381 tctctttcag accgcaccgg tacaggccat gaaacagccg ggtaaagggt tcgtcttgat
     7441 tattctcaac attatccttg cagtggtaat gtactacata tactattggt ttcaaactct
     7501 cgttgccggt atactgcccg ctggacctcc aggctatgtc caggagctct ggattgcaac
     7561 ggccctgctc ggttttacct ttcccgttat cactgcctac accgggtttt ttaacttctg
     7621 gcctttgacc gaatcaaagg cctcaaagga gtgacaaaaa atcgtatagt tttggttcga
     7681 atgcagctat aaagaaaagg cagtgctgaa acacttattt ataataaggg ctggatataa
     7741 aatccagccc ttattccatg gtatgtcacg aataatgtgg ggtaccttca tggaatactt
     7801 gtgtttaaat tctcccataa gccttggtga tggtgagagc tgttacccca aagtatctct
     7861 gtggaaaaga agcaacatgg atgccaagtt ctagaaatgt cctatccttt ttcatacttc
     7921 ataggataac atgagtcttg caaaatcact ctgcggcgaa gctgtaggtg tgtttgatta
     7981 caatcttagt tacttaacca aataaatcat gagacatggt gtttggtttt gctgagcaaa
     8041 ctgtcacaag acaaggaagc ttggcttttt gagcaagcaa aagttctgaa gttaagcgcg
     8101 agcgaaggtc accgcttgtg cgaatgagcg tgtttgaggg gcatacccaa cacggatgtt
     8161 catcctccta cccatccttt ttcaattgct gcaaaagttc tgaacgctgt gatagttcct
     8221 gtaacatatt tcgaaccttt gcttcaaggg cacgcgagat tgccaggaaa gcgtctttct
     8281 tgcttgccct gtattgggat gcaaaatcat tcaggttcac cggtgaacca accttgataa
     8341 ctgcctttct ctgcccccag aacctccttt ttccgaatgt ctcccgttca aggagtccca
     8401 caatatcaag aaaccgttcg gttgtcatcg ggagttcaag gtactggtgg tcgagggcca
     8461 taaagttggt tgctgtatcg aggctgtgac ggagcatctt tgccctctct cgcagggcct
     8521 ttgccgactc ttcggtgtct tttgcctggt tttgcctatg gatatactga tcgagagtgt
     8581 taatgagatc tcgaaggcga tcgggcagga tatgctcggg gtgatcttca acgccaatct
     8641 cttttgccgt tgcggcaatg atcacttcct tcatggtgct gatccgttga ctcagtgatg
     8701 tccctggttg aggcgatacg ccgtattcgt gttccttgga tacaagcatg gcttctccca
     8761 gagcgacgag acgctcctgg taatcttttt ttccctgatc tgttccaggg agcagttttg
     8821 tctcgagatg cctgagtgca cgatcgatgg ggagatccat gttgtgtata tagaatgttt
     8881 tgatggctat gggaacaaaa aaaatatcgg gtttctcccc ttttttggtc aactcatcat
     8941 aagctcgaaa agcaaattga cctatgcctg gctgaaaggg cattaacaca tcgcttgctc
     9001 cagttgagac tccttcagga aaaatgacca gccagttctt tccttttacg agaatttctg
     9061 atgttgcacg caaggaattc ctgtctggcg ctccgcggat taccgagtag caccccaggg
     9121 cctggaggag ccttccagca ggataataat cttcaaaaac ctcccgagcc gtaaggtagt
     9181 taaattcttc acccaccatc cgggaaaggg caaatattac atacggctct gtcatttctg
     9241 catggttaga aatcaaaata acccgtttcc ctttgaaact ttttaagaga gataggttga
     9301 ttggattgat ctctattctt gatatctttg gaatgagagg gagcataatg ggctcaagaa
     9361 tgtattgaac gaattttata aaaaggctgc aatttcttgg agggcgaaaa ggaaatatcg
     9421 ttgttgtatt catcctgtat ctcttcaacg tggactattt tcttttacaa aggcgagcat
     9481 aaatgccccg gtcacgaaaa gaaaaatgat tgcaaatatt gaacaacggg cattgccggt
     9541 gagcaaactt gtaataccca tgaggaaagg cccgaggact gccgcaaact ttccgagcat
     9601 gttgtagaaa ccgaagaact ctcctgcatt ttccgagggt atcatctttc catacaatga
     9661 tcggctcaaa gactgcactc ctccctgaac aaggccgaca gcaattgcaa ggacataaaa
     9721 ctcggcaggg tcattcatat aataacccca gatagccaca gcgacataga cagtaattcc
     9781 aataaaaata ccgattctgg tgccaagttt ttctccaatt ttaccaaaaa gaattgccgc
     9841 aggaaaacca ataaactggg tgattagaag ggccttaatg agaactgttt gatcgaaccc
     9901 caatgagagt ccgtagtcta ccgccatgat cactgttgta tcgacaccgt cgatataaca
     9961 ccagtaacca acgaggaaaa gaaaaatgtg tttaagtttt ctgatttctc ggaatgtgga
    10021 aaagagttgc ttcattccgg aacgcgccgc atctgtgaca gattgtgcag tccgtacctt
    10081 cggctccctg acaaaaagaa agatagggac ggagaataga gcccaccaga ctgctacgct
    10141 gaaaaatgat atgcggattg cctcaacagc agatgggaag ccgaatagat gtggtttcag
    10201 agccatgacc acattgatgg caaaaagaat cccgccaccg agatacccca gggagtatcc
    10261 aagggcagaa acaaaatcca tcttcttttt ttcaacaatg cataccatga gggaatcata
    10321 gaaaacattt ccaccggtaa agcctatatt ggcaaaggcg taaaggaata ttgccacgag
    10381 ccattcaccc tttgcaacaa atccaagaga acctgtcatc accataccca taaccgcaaa
    10441 aaaaagaaga aatcttttct tatttcccca ttgatctgca atggagccaa gaactggtgc
    10501 aagaagagct acaattacac ttgcaaggga gttagctgtt ccaaggttga atgtgctgac
    10561 atgaacatca gtgccagcac tccagtattg cttgaaaaaa actggaaaaa agcctgccat
    10621 gactgttgtt gcataagctg aatttgccca gtcataaaaa gcccacgaga tgactgtttt
    10681 tgtctcatag ccctgctttg tggcacctgc gtgcttcatc ttggtattag ggcggcagtc
    10741 cttgagaaca aacgatttta cgtttttgcc ttaaacccaa atcatgcatt agaacatctg
    10801 taaggtggtc tcgctgaccg tgtaatat
//
LOCUS       DAOOMC010000095        10786 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdus sp. isolate AMR_MDS_2685
            NODE_6922_length_10786_cov_6.229056, whole genome shotgun sequence.
ACCESSION   DAOOMC010000095 DAOOMC010000000
VERSION     DAOOMC010000095.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864760
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdus sp. (wastewater metagenome)
  ORGANISM  Syntrophorhabdus sp.
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae; Syntrophorhabdus.
REFERENCE   1  (bases 1 to 10786)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10786)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,729
            Genes (coding)                    :: 2,708
            CDSs (with protein)               :: 2,708
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 7 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10786
                     /organism="Syntrophorhabdus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6922_length_10786_cov_6.229056"
                     /isolate="AMR_MDS_2685"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2699756"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            608..3037
                     /locus_tag="PK125_09945"
     CDS             608..3037
                     /locus_tag="PK125_09945"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR02093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan phosphorylase"
                     /protein_id="HPB38463.1"
                     /translation="MQKDVETLRKSLMKNLTYNLAKDMYSATPRDKFQAIALSVREYI
                     VERWIRTQQRYYDVDAKRIYYLSLEFLLGRLLQNYALNIGLSDEYAKSVSALGVSYEE
                     ASELEWDAGLGNGGLGRLAACFLDSLATLQYPGYGYGIRYEYGIFQQRIKDGYQVEAP
                     DNWLRYGNPWEFPRPELLYPVRFAGKVDNAPGRIKGSTDWIGGEEVMAMAYDYPVPGY
                     KNETVNTLRLWAAKSIREFNLEFFHSGDYMKAVEERFSSENISKVLYPSDQYIAGKEL
                     RLKQQYFFVSATLKDIIRRYKKFHRRYDEFPDKVAIQLNDTHPSVGIAELMRILVDEE
                     GILWDDAWEITTKTFGYTNHTVLPEALEVWSEGLFSYLLPRHLQIIHEIDRRFLVQVA
                     AKFPNDGGRKDRMAIITGDKERNVNMARLALIGSHVVNGVSELHTNILKERVFKDFYE
                     MMPDKFKNVTNGITPRRWLQQANPLLAELITDAIGDRWTYDLNELKKIEPFAEDDEFC
                     RQFRRIKEANKKSLSDYLYNAYWLSLAPDYFLDCQTKRFHEYKRQLLNVFHIITLYNR
                     IRGGRADKNLVPRTILFAGKAAPGYFICKLIIKLIHSVSSFVDADPLTSDKLNIVFVP
                     NYDVSLAQRIMPAAELSEQISTAGYEASGTGNMKFTLNGALTIGTLDGANVEIREEVG
                     EDNFFLFGLTAEELVKARSSYNPRKYYEENKELKEVIDQIHGGYFSPAAPQLFHPITD
                     SLLRHDPYFVLADYGSYIQCQERVNETYRDQNSWIRMAILNVARSGKFSSDRAIHQYA
                     EKIWGTIPVSP"
     gene            complement(3063..3656)
                     /locus_tag="PK125_09950"
     CDS             complement(3063..3656)
                     /locus_tag="PK125_09950"
                     /inference="COORDINATES: protein motif:HMM:NF013856.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RlmE family RNA methyltransferase"
                     /protein_id="HPB38464.1"
                     /translation="MSKFIPKDKYFHRAKRNGYRARSAYKLKEIQEKLKLIRKGDKIL
                     DLGCAPGSFLQVLSEIVGAEGVVIGIDILPIVRLPQKNVVAIQGDIREIDIEELHILY
                     APGGFDVITCDISPNLSGIREVDNRNVGELFVAVENVVEKTLKVGGHLILKAFFSDDF
                     KIIKSKLANLFRTVSTFKPVASRSASSEIYLVAVEKT"
     gene            complement(3631..4734)
                     /locus_tag="PK125_09955"
     CDS             complement(3631..4734)
                     /locus_tag="PK125_09955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB38465.1"
                     /translation="MDTSTKMDNIISNINYIEQLEGNVDAFSQLSNDIKLELLDKLKF
                     ERTEIVGQFLNRIYTKEHDKQIQKIIKKLLFRLKTSGIKVEELRVEGESALKKYEEKR
                     VHRGLMSNYDGDGTRLAVVAFEAKRNTYVLVHSLLHFSRGLLELGNITVDREGLGQIF
                     TEYLKGSLKPFVIVEVAPRYAYYLIEEASRLSGQYADEIKQMKSFSYRLGGRVQKPSD
                     VYVLPIPNDIESSSLDHILSNSLFEPFFVIWDTLEDDKKQFNDIGASSSIVLPPYLME
                     EKKQALIKNLIENGKLSPNLPFMKRLMEDYAYIFYTLGDFKSFKGLVDILQLSDGPYK
                     MLSFFVKKALREEEKAQEHGLIINPYEQVHPQR"
     gene            complement(4761..4967)
                     /locus_tag="PK125_09960"
     CDS             complement(4761..4967)
                     /locus_tag="PK125_09960"
                     /inference="COORDINATES: protein motif:HMM:NF012621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HPB38466.1"
                     /translation="MAEANITIEGMSCQHCVMAVKKALGGVPGILESNVQIGSAVVKY
                     DESKIRKEDIETKIEDAGYKVTNK"
     gene            complement(5075..5947)
                     /locus_tag="PK125_09965"
     CDS             complement(5075..5947)
                     /locus_tag="PK125_09965"
                     /inference="COORDINATES: protein motif:HMM:NF015382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carcinine hydrolase/isopenicillin-N
                     N-acyltransferase family protein"
                     /protein_id="HPB38467.1"
                     /translation="MSFAYVKNIFLVVICLFILFREGTCAFACSLLAATGDRLKDGGT
                     LVAMTWDVPRNIKGELRLVIPRKGSPYLGLFPLQAKNEGNIVAGLNEPGLVLITASVD
                     SKPSKKGFLRRCSLVETILRSFGTVDAVIANTNLISNAPPLFFIIADKSKIALIQIGS
                     RGRYTVDCIENGLLYQTNHYTHQNLLRENEIYVENSILRYNRLQNLLANQSTHFKVED
                     FLSLADDRGNGPDNSIWRWGSSEKKDRTLARWVVYLSKNSVPELYFRLLNPGNNELNY
                     EIKTDNAFWTEGIE"
     gene            6162..7901
                     /gene="thrS"
                     /locus_tag="PK125_09970"
     CDS             6162..7901
                     /gene="thrS"
                     /locus_tag="PK125_09970"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HPB38468.1"
                     /translation="MAEEQKEHDNVHLDMLRHSTSHVMAQAVRDLFPDAKVAIGPSIE
                     NGFYYDFDYEPGFTEEDLSKIENRMKELVKMDLPIVRKMVGKEEAIALFRSMGENFKV
                     ELIEDIGDTEVSLYTQGNFTDLCRGPHLPSTGKLGAFKLLSLAGAYWKGDEKNKMLTR
                     IYGTAFPDEASLNNYLNFLEEVKKRDHRRLGKELDLFSLSDDVGAGLVIYHPNGALLR
                     YILEDFERKEHLKRGYQFVVGPHILKLDMWKRSGHFDNYRDNMYFTKIDDVDYGIKPM
                     NCLSHIMVYRSKIRSYRDLPLRYFELGTVCRHEKSGVLHGLLRVREFTQDDAHIFLRQ
                     DQLHEEILNIIQFVRDVMAIFNFEYEMEISTRPEKYIGTLEDWNKAEQVLKSVLVSQD
                     IPFDINEGDGAFYGPKIDVKLKDAIGRKWQCATIQCDFALPERFDLTYVDSDSKRKRP
                     VMLHRVILGAMERFIGILIEHYGGRFPVWLAPTQVIIMSITDEQASYVSALHANMIHE
                     GIRAEVDTRNEKLSLKIREGVVKKVPYLLIVGKKEMANDTVSVRVRDGGELKDVAIKD
                     FIERVKDENLLRR"
     gene            7898..8422
                     /gene="infC"
                     /locus_tag="PK125_09975"
     CDS             7898..8422
                     /gene="infC"
                     /locus_tag="PK125_09975"
                     /inference="COORDINATES: protein motif:HMM:TIGR00168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HPB38469.1"
                     /translation="MSLIGKDIKDVNINERIRVREVRLIDESGRQLGIVPTLDALRMA
                     REQELDLVEVSPKTVPPVCKIMDYGKYKYQLAKKAAEAKKKQTVIQIKEMKLGLKIDE
                     HDLGFKIKHIRGFLDDGAKVKVIVMFKGREMLHVDMGKKLAQKIIESVKDVGTLEQEQ
                     KFDGRNIVMIFAPL"
     gene            8434..8631
                     /gene="rpmI"
                     /locus_tag="PK125_09980"
     CDS             8434..8631
                     /gene="rpmI"
                     /locus_tag="PK125_09980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001830767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HPB38470.1"
                     /translation="MPKLKTHRGFAKRIKVTGKGKLKRAKAFHSHLLTGKTPKMKRRL
                     SRPDTVHPSDAKRVKTLIPYL"
     gene            8652..9029
                     /gene="rplT"
                     /locus_tag="PK125_09985"
     CDS             8652..9029
                     /gene="rplT"
                     /locus_tag="PK125_09985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004192938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HPB38471.1"
                     /translation="MPRAKRGFKARRRRKKILSLARGMYDRRRSAYSIAKRSVYKAMK
                     YSFIGRKQKKRDFRSLWITRINAACRSQGISYSRFMNGLTVANITLNRKSLADMAIND
                     PAGFENIVVKVKQVMSARPSVNA"
     gene            9125..10135
                     /gene="pheS"
                     /locus_tag="PK125_09990"
     CDS             9125..10135
                     /gene="pheS"
                     /locus_tag="PK125_09990"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HPB38472.1"
                     /translation="MNIDELRNRIESEITSLKTFEDVKKVKASLLGRKGLFSEYLDGL
                     KLLDRDEKKIFGKEINLLKMHAESRLKELESFYLEEEKRKKEALDKIDITMPGKIPLL
                     GKKHPITQTLEEIIRIFTSLGFSVAEGPDIEQEYYNFEALNIPKDHPARDMQDTFYIK
                     SDIVLRTHTSPVQIRTMESQLPPVKIIAPGSVYRCDNDISHTPMFHQVEGLMVDKRVR
                     FSDLKGILSIFIREIFGENTPLRFRPSYFPFTEPSAEVDIGCVICEGDGCRVCKETGW
                     LEILGSGMVHPQVLRIVGYDPEEVSGFAFGMGVERIAMLKFGIDDIRQFYYNDIRFLS
                     QF"
     gene            10149..>10786
                     /locus_tag="PK125_09995"
     CDS             10149..>10786
                     /locus_tag="PK125_09995"
                     /inference="COORDINATES: protein motif:HMM:NF013734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB38473.1"
                     /translation="MRIPYEWLNEFVVLDINPYELASKLTMRGFEVEAIEEYKPSLDG
                     VCVGEVVKIDLHPNAKNLTVCKVRTVNEALPIVCGAKNVKEGDKVPVAMIGSTLANGL
                     LIEKKNIKGVESLGMLCSEKELGLSDDHSGIFILPGEMKAGTKLDELTGLQDFVFDIN
                     VPPNRGDCLSVYGIAREVGSILNQRAKLPMFKLNGLSNKEISNLISLSVKDT"
BASE COUNT         3325 a         2013 c         2369 g         3079 t
ORIGIN      
        1 ttccaaaatg ctttagtaag cagataaaag aattctgtgg taatcatcaa ttcaacattg
       61 acctgaattc tatcactttt tctgtggtca atattttgct tctgtcttgt ttctcatttt
      121 ttatctcgca agtgtttgta ttgaaagcat tatatgagct ttatttctgt tcctgcgctt
      181 gtttgaagtt gcttttaacg tgtcaaaaaa agagcaaatc tcaaaacaat ctggtaaaat
      241 cgaattatca ttgttaagga ttcttgactt tccaacagtg gaacataata ttgcgtaaaa
      301 cataaaaagg gatcaatatc tgcaaaaaag ggaaccagta tactcctgat gaaaatgaat
      361 gtccttggat atatctcaat gcgtttggag taaaaatgcc cagatacaaa aaagatggat
      421 aattaacagc gaggaaagaa tagaaattaa tgaaatgctg acgacagtaa aagaaagggt
      481 tcatgccaag gaaattacgc ttcatcggat ggtatcttat ctggatggag atatacattc
      541 ttttttttgc aacattcttt ttaaagtgat attattcgga taccgaaaac aaccatagga
      601 tttttttatg caaaaagatg ttgaaacgtt gcgcaagtca ttaatgaaaa accttacata
      661 taatcttgcc aaggatatgt actcagctac accaagagat aagttccagg ctattgcact
      721 atcagtgcgg gagtatattg tagaaagatg gattagaact cagcagcgtt attatgacgt
      781 agatgcaaaa agaatctatt atttgtcact tgaatttctt ctggggaggt tattgcagaa
      841 ttatgccctc aatataggac tctctgatga gtatgcgaaa tcagtcagtg cccttggagt
      901 atcctacgaa gaagcatcag agcttgagtg ggacgctggt ctcggcaatg gtggtctcgg
      961 aaggcttgca gcatgtttcc ttgattcttt agccactctt cagtatcctg gttatggtta
     1021 tggaatacgt tatgagtatg gtatttttca gcagagaata aaagatggat atcaggtaga
     1081 agccccagat aactggctgc gctacggtaa tccttgggag tttccaagac cagaacttct
     1141 ctacccagta aggtttgcag gaaaagtgga caatgccccc ggtaggataa agggtagtac
     1201 ggattggatt ggtggtgagg aagtcatggc tatggcctat gattatcctg tccccgggta
     1261 taaaaatgaa actgtgaata cactccgctt atgggctgcg aagtcaatta gagaatttaa
     1321 tctggagttt ttccatagtg gcgactacat gaaggctgtt gaggaacgtt tcagcagtga
     1381 gaatatatcg aaagtactct atccgagcga tcagtatata gcaggaaaag agcttagact
     1441 gaagcaacaa tatttttttg tgagcgcaac cttaaaggac atcatacgga gatacaaaaa
     1501 attccataga cgatatgatg aatttcctga taaggttgcc atacaactaa atgatacaca
     1561 cccatctgta ggtattgccg aacttatgag aatacttgtg gatgaggaag ggattttgtg
     1621 ggatgatgca tgggaaatta ccacgaaaac cttcggatat acaaatcata ccgtgttgcc
     1681 tgaggctctt gaagtatggt ccgaaggact tttctcgtat ttgctccccc gccatctcca
     1741 gattatccac gagattgaca ggcggtttct tgtccaggtt gctgcaaaat ttcctaacga
     1801 cggcgggcga aaggatagaa tggcgatcat aacaggggac aaagaacgga acgtgaatat
     1861 ggcgcggctt gcccttatag gaagtcatgt tgtcaatggt gtgtctgaac tccacacgaa
     1921 cattttgaaa gaaagggttt ttaaagattt ttatgaaatg atgcccgaca agttcaaaaa
     1981 cgttacaaac ggcatcacac caagacgttg gcttcaacag gccaacccgt tgctcgctga
     2041 actcattacc gatgccatag gagacagatg gacatatgat ctaaatgaac taaaaaaaat
     2101 tgaacccttt gccgaggatg atgaattttg tcgacagttt agaaggatta aagaggcaaa
     2161 caaaaagtcc ttatccgatt atctttacaa cgcttactgg ctttcgttgg caccggacta
     2221 ttttcttgat tgccagacca aacgattcca cgagtacaaa cgtcagctcc tcaacgtttt
     2281 tcacattata actctttata ataggattag gggaggcagg gcggacaaga accttgttcc
     2341 gagaacaatc ctcttcgctg gcaaggctgc gcccgggtat tttatttgta aactcataat
     2401 taagcttatt catagtgtta gcagttttgt tgacgctgat cccttaacga gtgataaatt
     2461 aaacatagtt tttgtaccaa attatgatgt gagtctggca caacgaataa tgccggcggc
     2521 agaattatca gaacagattt ctactgctgg atatgaggcg tcagggactg gaaacatgaa
     2581 atttactcta aacggcgcct tgactatcgg gacactggat ggtgctaatg tcgaaattag
     2641 agaagaagta ggggaggata atttttttct tttcgggtta accgctgaag aattggtaaa
     2701 ggctcgttca agctataacc cacgtaaata ttatgaggaa aacaaagaac tgaaagaggt
     2761 aatcgatcag atccacggag gatatttttc acctgcagca ccacaacttt ttcatcccat
     2821 aactgattca ctgctccgac atgacccata tttcgtttta gcggattatg gttcatacat
     2881 ccaatgtcag gagagagtca atgagacata cagagatcaa aatagttgga taaggatggc
     2941 gattcttaat gtggcccggt caggtaaatt ttctagcgac cgtgcaatcc atcagtatgc
     3001 agaaaaaatt tggggaacta tcccggttag tccttagatc agacacaata ggtcagcagt
     3061 attcaagttt tttcaacagc caccagatag atttcggagc tggcgcttcg cgaagcaaca
     3121 ggtttgaatg tcgagactgt tctgaacaaa tttgcgagct tcgattttat aattttaaaa
     3181 tcatcagaaa aaaaagcttt tagaataaga tgacccccaa ccttgagagt cttttcaacc
     3241 acattttcaa cggccacaaa gagttcgcct acatttctgt tatccacctc cctgataccg
     3301 gaaaggttag gggagatgtc acaggtaatt acatcgaaac ctcctggtgc ataaaggatg
     3361 tgcaattcct caatatctat ctctcggata tcgccctgaa tggcgacaac attcttttgt
     3421 ggcagccgga caatggggag gatgtcaatg ccgatgacaa cgccttcagc accaacaatt
     3481 tctgaaagaa cctggagaaa actccctggc gcgcagccta ggtctaaaat tttgtcacct
     3541 tttctaataa gtttaagttt ttcttggatt tctttaagct tataagcgct gcgagccctg
     3601 tatccattac gtttagctct atggaaatac ttatctttgg ggatgaactt gctcataagg
     3661 atttatgatg agaccatgct cttgagcctt ttcttcttcc ctcagtgcct ttttcacaaa
     3721 aaatgatagc attttgtagg gtccatccga tagctgcaat atatcaacca accctttgaa
     3781 agatttaaaa tcacctagtg tgtaaaaaat gtacgcgtaa tcttccatga gtcttttcat
     3841 aaaaggcaag ttggggctga gttttccatt ttcaatgagg tttttaatta atgcttgttt
     3901 tttttcttcc atcaaataag gaggcaaaac aattgatgaa gaagctccta tgtcattaaa
     3961 ttgtttttta tcatcttcca atgtatccca tatgacgaaa aaaggttcaa agagactgtt
     4021 tgaaaggatg tgatccagag aagaactctc tatatcattt ggtattggga gaacataaac
     4081 atcagaaggt ttttgaactc gaccaccgag tcgataggaa aaagatttca tctgcttaat
     4141 ctcatctgcg tattgaccgg acaaacgtga tgcctcttca atgaggtaat atgcatatcg
     4201 aggagcaacc tcaacgatca caaaaggttt taatgagccc ttcagatatt cagtgaaaat
     4261 ctgcccaagt ccttctctat ctacggtaat attgcccagt tccagaagac ctctggaaaa
     4321 atggaggagg ctatggacca gaacataggt atttcttttt gcctcaaaag caaccactgc
     4381 cagacgtgtt ccatcgccat cataattaga catcaatcct cgatgcacac gtttttcctc
     4441 atatttttta agggcggatt ctccctcaac tctgagttct tccactttga tgccacttgt
     4501 tttaagtcga aatagaagtt tcttgataat tttctgaatc tgtttgtcat gctctttagt
     4561 atagattcgg tttaagaact gaccaacaat ttctgttcgt tcaaatttta atttatcaag
     4621 taattctaat ttgatatcat ttgaaagttg agaaaaagca tcaacatttc cctctaattg
     4681 ttcaatataa tttatatttg aaataatatt atccatttta gtgctggtat ccatttccgt
     4741 attaacatca aaaggataga ttacttgttg gtgactttgt atcctgcatc ctcgattttt
     4801 gtctcgatat cctctttcct gatcttgctt tcatcatatt ttactactgc gcttccaatc
     4861 tggacattgc tttccagaat gccgggcaca cctccgagag cctttttaac tgccatcacg
     4921 cagtgctggc aactcatccc ttctatagtt atattagcct ctgccatacc aacctcctta
     4981 atcggtaatg tgtttcttaa ttatagcatg aataaatgtc gatctgtgat aaagtgcttc
     5041 aaatggtgta atgccgaaaa tataacattg tggctcattc tattccttca gtccaaaacg
     5101 cattgtctgt ttttatctca tagtttaatt cattgtttcc tggattcaat agcctgaaat
     5161 atagttctgg aacagagttt ttagaaagat acaccaccca tcttgccaat gtacggtctt
     5221 ttttttctga acttccccac cgccagatac tgttatcagg tccattaccc ctgtcatcag
     5281 caagactgag gaaatcttct actttaaaat gtgttgattg gtttgcaaga agattctgta
     5341 acctgttata gcgcagaata ctgttttcaa catagatctc attttctctg agaaggtttt
     5401 gatgtgtgta atgatttgtt tgatataata atccattttc aatacaatca actgtatacc
     5461 ttccgcgaga accaatctgg ataagcgcga tttttgactt atctgcaatg ataaaaaaca
     5521 aagggggagc gttagaaata agattggtat tagctatcac agcatcaacc gtaccgaagg
     5581 atctcaaaat agtctcgaca agactacacc ttctaaggaa tccttttttt gaaggcttgg
     5641 agtccacact ggcagtgatt aaaacaaggc caggttcatt aagtcctgca acaatgttgc
     5701 cttcattttt tgcctgtaaa gggaagagac caaggtaagg cgaccctttc cgagggatta
     5761 caagcctcaa ttcacctttt atattccggg gaacatccca tgtcatggca acgagcgtcc
     5821 caccatcctt taatctgtcc ccggttgctg ccaaaagaga acatgcaaaa gcgcatgtcc
     5881 cttccctgaa aaggatgaat aaacaaataa caacaaggaa aatgtttttt acatacgcaa
     5941 atgacatatc agccttgttc cgatattata tacttcaaaa atctattgta aaaggagaat
     6001 tttgaaccta actttttcat ggtagagtat ttcttcgaac atttgtaaga tagcgagccc
     6061 aaagatgatt tcttcaagat attgccaagt taatgattga caaggaaaag ctaaaaatgt
     6121 taagaatatc tcctaattta cttaatgatt ttggagtctt aatggctgaa gagcaaaaag
     6181 agcacgataa tgtacattta gatatgcttc gccacagcac gtcccatgta atggcacagg
     6241 cagtgcggga tctttttcct gatgcaaaag ttgcgattgg gccgtctatt gagaacggct
     6301 tttactatga ttttgactat gaacccggct tcacggaaga agatctttcc aagatcgaaa
     6361 accggatgaa agaattggtt aagatggacc tgcctatagt taggaagatg gtgggaaagg
     6421 aagaggctat tgccctcttt cgctccatgg gtgaaaattt taaagtcgaa cttattgagg
     6481 acattggaga tactgaggtc agcctatata cccagggcaa cttcactgat ctttgcaggg
     6541 gtccccattt gccatctacc ggaaagttag gtgcatttaa acttctgagc cttgcaggcg
     6601 cttactggaa aggcgatgaa aagaataaga tgcttacaag aatctatggt acagcctttc
     6661 cggatgaggc atccctcaac aattacctca actttctcga agaagtcaag aaacgagatc
     6721 atagaaggct tgggaaggag ctggacctct tcagtttgtc tgatgatgta ggcgctggac
     6781 ttgttatcta tcatccaaat ggggctcttc tcagatatat ccttgaggat tttgaacgga
     6841 aagaacattt aaaacggggc tatcagtttg tggttggccc ccatatcctc aagctggata
     6901 tgtggaagag gtcaggccac ttcgataatt atagggataa tatgtatttt acgaagattg
     6961 atgacgttga ttacggcatt aagcccatga actgtctctc acatattatg gtctaccgat
     7021 ctaaaataag gagttaccgc gaccttccgc ttcgatattt tgagcttggc actgtctgcc
     7081 gccatgaaaa atcaggagtc ctccacggtc ttttgagggt aagagagttt acgcaagatg
     7141 atgcccatat ctttttgaga caggaccaac tacacgaaga gatattgaat atcatccagt
     7201 ttgtccgtga cgtcatggca atctttaact ttgaatatga aatggagata agtactagac
     7261 ctgaaaaata tattgggacc cttgaagatt ggaataaagc agagcaggtg cttaaaagtg
     7321 ttctcgtaag ccaggatatt ccgtttgaca tcaatgaagg ggatggtgct ttctacggac
     7381 ccaaaataga cgtaaaattg aaggatgcca tagggagaaa atggcaatgt gctaccatac
     7441 aatgtgattt tgcactgcct gagagatttg atcttacgta tgttgacagc gatagtaaaa
     7501 gaaaaaggcc tgtcatgctc catagggtaa tactgggtgc catggaacgt ttcattggaa
     7561 ttcttattga gcactatggt ggacgttttc ctgtctggtt agcaccaacc caggtaatta
     7621 tcatgagtat aaccgatgaa caggcatctt atgtgtcggc gttacatgca aatatgattc
     7681 atgagggtat acgggccgag gttgacacaa gaaatgagaa actcagtctc aaaataaggg
     7741 aaggagttgt aaaaaaagtt ccctatcttt tgattgttgg caagaaggag atggcaaacg
     7801 atacagtttc cgtcagggtc agggatggtg gggaactgaa agatgtagct attaaggatt
     7861 ttattgaacg tgtaaaggac gaaaatcttc tcaggaggtg agcctcatag gaaaagacat
     7921 caaagatgta aatattaatg aaagaataag ggtcagggag gttcgactga tcgatgaaag
     7981 cgggagacag ttgggtattg taccaacttt agatgccctc aggatggcaa gggaacaaga
     8041 gcttgatctt gtggaagtct ccccaaaaac tgtaccgcct gtttgtaaga ttatggatta
     8101 tggcaaatac aaataccagc ttgccaaaaa agcagctgag gcgaaaaaga agcagacagt
     8161 gatacagatc aaggaaatga agcttgggct taagattgat gagcatgatc ttggattcaa
     8221 aatcaagcac attcgagggt tcttggatga tggcgcgaag gtgaaagtga tagtgatgtt
     8281 taagggtagg gaaatgctcc atgtggatat ggggaaaaag cttgcccaaa aaattattga
     8341 gtcagtgaaa gatgtaggaa cacttgaaca agaacagaaa ttcgatggta gaaatattgt
     8401 tatgattttt gctccacttt aaaggaggcg tatatgccga agttgaaaac acacaggggt
     8461 tttgctaaga ggatcaaagt gaccggtaag ggtaagctca agagggcaaa agcctttcac
     8521 agtcaccttc ttactggcaa gactccaaag atgaagagac ggctttccag gcctgataca
     8581 gtacatccgt ccgatgcaaa aagagtaaaa acattaatac cttacttata actgaatagg
     8641 agtgaataga aatgccaagg gcaaaaagag gatttaaagc aaggagaaga cgaaaaaaga
     8701 tattgagcct tgccagggga atgtatgatc gtagacgcag cgcatatagc atagcgaagc
     8761 gatcagtata caaagcaatg aagtattcat tcatcggaag aaagcagaaa aaaagagact
     8821 tccgttcatt atggataaca aggattaatg cggcctgtag gtcacaaggc atatcataca
     8881 gcaggtttat gaatggactt acggtagcca acataacttt gaacagaaaa tcactggctg
     8941 atatggcaat caacgatcct gcaggcttcg aaaatattgt tgttaaagtt aagcaagtaa
     9001 tgtccgcccg accctcagta aatgcataat tgagaccgaa gttacagggg gttcatataa
     9061 cccccgggcg aatcaacagc tgtaacatgc atatatccaa gccataatgc aacctgggtg
     9121 aagcgtgaat attgacgaac tgcgaaacag aattgagagt gagataacct cattaaaaac
     9181 ctttgaagac gtgaaaaagg ttaaggcatc ccttcttggc cgaaaaggtt tgttttctga
     9241 gtatctcgat gggttgaagc ttcttgacag ggatgagaaa aagatttttg gcaaggaaat
     9301 taatttgttg aaaatgcatg ctgaaagccg actcaaggaa ctggagtctt tttatcttga
     9361 agaagaaaaa agaaagaagg aggcccttga caaaatagat attaccatgc cgggaaagat
     9421 tcctttgctc gggaaaaagc atcctataac ccagacgttg gaagaaatca taaggatttt
     9481 tacgtctctg ggattttccg ttgctgaggg gcctgacatt gaacaagaat actacaactt
     9541 tgaggctctc aatattccca aagatcatcc tgccagggat atgcaggata cattttatat
     9601 caaatcagat atagtgttga gaacccacac atcgccagtt caaataagga ccatggaatc
     9661 ccaacttcca ccagttaaaa tcatagcccc tggttctgtg tatcgatgtg ataatgatat
     9721 ctcacacacc cctatgttcc atcaggttga gggtctaatg gttgataaac gagtccgttt
     9781 ttccgatctg aaaggcattt taagcatatt tatccgcgaa atatttggag agaacacccc
     9841 acttcgattc aggccaagct actttccttt tactgaacca tctgcagaag tggacatcgg
     9901 gtgtgttatt tgtgagggcg atggatgcag ggtctgtaag gaaacagggt ggctcgaaat
     9961 attgggttct gggatggtac acccccaggt gttgagaata gtcgggtatg acccagaaga
    10021 agtttccgga tttgcttttg gaatgggtgt tgaaaggata gctatgttaa aattcggtat
    10081 tgatgacatc aggcagtttt attacaatga catcaggttt ctttctcaat tttgaggtgg
    10141 tattttaggt gaggattcct tacgaatggc ttaatgaatt tgttgtccta gatataaacc
    10201 cgtatgaact tgcatcaaaa ctgaccatgc gcgggtttga ggtcgaggcg atagaggaat
    10261 ataagccctc ccttgatggg gtgtgtgttg gtgaagttgt caaaatagac cttcatccca
    10321 atgcaaaaaa cttaacagtt tgtaaggtta gaactgtaaa tgaggcgctg ccaattgtat
    10381 gtggggcaaa gaacgtaaag gaaggcgata aagtccctgt tgctatgatt gggtcaacct
    10441 tagccaatgg tttattgatt gaaaaaaaga acattaaagg tgtggaatcc ttgggcatgc
    10501 tctgttccga aaaggaactg gggctttctg atgaccatag tggtatcttt attctgccgg
    10561 gagaaatgaa agctggtacc aaactcgatg agctgaccgg tcttcaggat tttgtgtttg
    10621 atatcaatgt ccccccgaat aggggcgatt gcctttcagt gtatggtatt gcacgggagg
    10681 taggcagtat tttgaatcag agggccaaac tgcccatgtt taaattaaac ggcttatcaa
    10741 ataaagagat aagcaacctg atatcgcttt ctgtcaaaga cacaga
//
LOCUS       DAOOMC010000096        10772 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdus sp. isolate AMR_MDS_2685
            NODE_6936_length_10772_cov_5.342260, whole genome shotgun sequence.
ACCESSION   DAOOMC010000096 DAOOMC010000000
VERSION     DAOOMC010000096.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864760
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdus sp. (wastewater metagenome)
  ORGANISM  Syntrophorhabdus sp.
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae; Syntrophorhabdus.
REFERENCE   1  (bases 1 to 10772)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10772)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,729
            Genes (coding)                    :: 2,708
            CDSs (with protein)               :: 2,708
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 7 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10772
                     /organism="Syntrophorhabdus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6936_length_10772_cov_5.342260"
                     /isolate="AMR_MDS_2685"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2699756"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..868
                     /gene="dctP"
                     /locus_tag="PK125_10000"
     CDS             <1..868
                     /gene="dctP"
                     /locus_tag="PK125_10000"
                     /inference="COORDINATES: protein motif:HMM:NF015445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein DctP"
                     /protein_id="HPB38474.1"
                     /translation="KLSGGKIKLSVIGSYRAGGEAAMVDKMGVGGIDGALMTIVGLSK
                     INKDAAGLSNIPMIYKSLDEVDFVEEKFGAELSKTMEQKGFIVLFWTDIGWIRHFSVH
                     PIMYPHDLKKMKMFTWAGSPEQVDIMKEWGTNPVSLEPSDILSSLSTGMINVVSATPF
                     SANAGQYARVTKYMLEINWGPLVGGAVIRKQVWEKIPAGLREEFLKVAAETGREIKEA
                     GRKESDDAVEVMRKKQGLHVVVPSPQINEEWRQAVRAVYPKIRSSMVTAESFDRVESL
                     LRGYHGKSTANK"
     gene            843..2774
                     /locus_tag="PK125_10005"
     CDS             843..2774
                     /locus_tag="PK125_10005"
                     /inference="COORDINATES: protein motif:HMM:NF018513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0015556 - C4-dicarboxylate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HPB38475.1"
                     /translation="MEKVLQTNSLDSLGFRRRPGTCLLARLCRGKERRSLSSTAANIF
                     EGLENGLIVMALGLMMALPLAESLLRRVFGVGITGSAVFVQHLVLYVGMLGSVIAARK
                     GRLLSISFVTTYLQGKWKTAASVYANLFAVVITCYLVIASIQFILAKKIVGDTLIHGV
                     PLWPILLILPFGFALVACRTFLQVCSSWTIRCVAFLIITAMLSIGTVVSAKSLVLPGI
                     VGLLLASVLGAPIFTVLGGAAMILFWGNGQPIAMVPINHYRLTVDATLPVIPLFTLVG
                     YFLAESGAAQRLLAIFQTIFSKMRGGAVIITTVVCALFTAFTGASGVTILALGGLLMP
                     ILISSRYSQRNTLGLLTSAGSLGLLFPPSLPPILYAVVATTIGSEITIGSIFLAGLIP
                     GVLLLVLTWWWGIRSDPSTYIQEALSGTKALKAVLQAKWELLLPVVALVSLFSGIATP
                     VEAAAFTALYALIIEVCVYRDLKFFRDVPRVMVECGLLVGGVLIILGVAMGFTDFLFT
                     VSVPERLVEWAKNSIHSKWLFLIALNIFLLVVGCLMDIYSAIIVVAPLIIPLGREFGI
                     DPLHLAIIFLANMELGFLTPPVGMNLFLSAYRFDVPMSEVIRSILPILLVLLGGVLLI
                     SYVPFLTTALPHLFQAVGR"
     gene            2815..6996
                     /locus_tag="PK125_10010"
     CDS             2815..6996
                     /locus_tag="PK125_10010"
                     /inference="COORDINATES: protein motif:HMM:NF013986.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008047 - enzyme activator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydratase activase"
                     /protein_id="HPB38476.1"
                     /translation="MYRIGLDIGSVSVNVIVMNEENTIVSSRYIRHMGRPFQAGKKAL
                     EKISENFEVDFVSTTGTGARDFAALIGASFVNEIVALAKAFNHLYPNVRSVIDIGGED
                     SKLILFEQDGQNERIRVKDFSMNALCAAGTGSFLDQQASRLLFTIEEFSEVALKSTNP
                     PRIAGRCTVFAKSDMIHLQQIATPDYEIVAGLCYALARNFKGNIAKGRDIAAPVAFVG
                     GVAANAAMRRALMDVFSLNDTQFFVPQHFTSMGAAGALFAVLDEPSLRNRYLGLGSLN
                     DCLMNKKVRAAHEPLVISNENLRVSYDMKPVDGKTEVYLGVDVGSISTNLVVIDKEKN
                     VLAKRYLMTEGRPLEAVKKGLAEIADEVGRQVEILGAGTTGSGRYLTADFIGGDIVRN
                     EITAQAEAAIHIDPEVDTVFEIGGQDSKYISIDNGVIVDFEMNKACAAGTGSFLEEQA
                     ERLGISIKEEFGNLALSAKKPVKMGERCTVFIESDLVHHQQMGEGTDDIVSGLSYSIV
                     ANYLNKVVGDRKVGDKIFFQGGTAFNKGVIAAFEKTVGKPITVPSHHDVTGAIGVALL
                     AMKEKSWDHSNFKGFDLSKRPYSIETFECKGCENLCEIRKVTVENESPLFYGSRCEKY
                     DVVRRSEKRNMPDLFKVRDELLHSTYDRTIDGEPIGVPKILNMWEFMPFWKAFLTELG
                     FSPVFSDPTNKKTIREGVESIIVETCFPIKLTHGHILNIINKGIKRIFIPSVINLRGT
                     SKNAPNSFACPYAQSIPYSIRASIDFDAHGVQMDTPIVYFGKNKSEILDCLVSYGKEI
                     NRSKIEVKKALTTAFEVQETFYRKIAQAGTDALSALNDNDKAMVIIGRPYNSADPGAN
                     LNIHRKLMALGVQPLPMDMMPIVENAVIDGDLENMYWGYGQKILRTARSIKDLPNVYG
                     IYLTSFGCGPDSFITHFFKRIIRGKPFLQLEIDEHSADAGIVTRLEAFLDSIKNAKQN
                     STISKSRLPHVATKSHRRIIYIPNMSDHTIAFKSAFRACGQEAEVMDEPDDQSVYWGR
                     KYTSGKECYPCILTTGDMVKKVKQSDFDPERSAFLMPSGNGPCRFGQYHRFHRMILDE
                     MGFENVPVYAPNQDDKLYKDFNIVGNRFSKLGWQAIVATDMLIKMLHEIRPMEIIRGD
                     AERVYYKSIESIAYSIEKGNNGIVNALHDSINRFLQIKRNEIPRPIVGVVGEVYVRCN
                     RFSNNDVIKKVEELGGQAWFAPLTEWISYINFSNKRRKSVRKRSITHVLRTSLTEYIQ
                     GKEERRLEEPFMGLIQYGEEPSVNSVIDKASLYIDPSFEGEAILSVGKAIDFIDKGVS
                     GIINVMPFTCMPGTITSAVLRLVQKKYNIPIINVAYDGQGLTNIDTRLEAFMYQVREH
                     FADSDKMSGSMSLQL"
     gene            complement(7002..9224)
                     /locus_tag="PK125_10015"
     CDS             complement(7002..9224)
                     /locus_tag="PK125_10015"
                     /inference="COORDINATES: protein motif:HMM:TIGR02074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HPB38477.1"
                     /translation="MKRKILIYMVSMVAFSVILLATGFYIYIMKDIPSVKVLKRLENK
                     PVSSIYGANGELIYLIVPDNRIFIDYNKIPQHVKDAFLAAEDAEFFKHGAIDFQSVVR
                     ALWKNITSGRVVQGGSTITQQVIKSLLLGPERSILRKTREAILSYRLEGSLTKKEILN
                     LYLNNVYMGHGVYGVEAASQIYFGKHVWNVTRAEAALLAGIVQAPSRYTPKKYPGAAR
                     VRQEYVIGQMAEKKFIDERRRNEILKEKVNIRENNGVFTDSYFKDYVLKSVEEKIGKG
                     VLATRKLSIYTTADAGLQRLAEDAVKRGLNLYEQRKGAYSVVSHIDRKKWNNYKKARE
                     RDYKQVGLKAGKQYNLLVSDKLDNGYAVFAGNEKGILKMDAFPYKPGDIIKATYDGID
                     GEKIHVFHPIKTVKVEGALISLDVQTGYILAMVGGRDFEQSPYNRAIAAKIQSGSAFK
                     PFIYLAALKKGYSIDTMLVDEPKEYSMGMGKAWTPRNYDGKYVGQISMRDAVAYSKNA
                     ATVRLLEEVGVNAVKEVMTDLGIDADLPHELAIALGTSNLTLLDLVKGYAVFANGGLR
                     VKPIGVLKVVDSTGNTLMENHEEKVRVLSEDIAYNMNVLLKGPTEYGTAKGAARLGYP
                     VAGKTGTTSNYYDALFIGYSPHVITGVWVGFDTRTSLGEKESGARVCLPIWMSFMGTA
                     LRRYPPDDFAAPSVFSPILEWFRGFRSDGGSPPRPDQRPETVPNRRQEPAPEPWPRIH
                     "
     gene            complement(9390..9785)
                     /locus_tag="PK125_10020"
     CDS             complement(9390..9785)
                     /locus_tag="PK125_10020"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF025892.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HPB38478.1"
                     /translation="MKLDLPLQLAEQSPYYKHLGITIVEAREGFAKLRLNFMECLTHP
                     FGYLHGGAVASLADSAGINAVLTMLKDEQKALTLEMKINYLLPVKDTAIFAEARIVHK
                     GKKFALADVDIKNSDGQLVAKAIVTCAIM"
     gene            complement(9853..10725)
                     /locus_tag="PK125_10025"
     CDS             complement(9853..10725)
                     /locus_tag="PK125_10025"
                     /inference="COORDINATES: protein motif:HMM:TIGR02249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integron integrase"
                     /protein_id="HPB38479.1"
                     /translation="MRRFIAFHDYTDLKKLDATKAVREYLEYLAVERAVAASTQNQAL
                     NALVFLYTNVLNKPFGDLETFVRAKRPQRLPEVMTREEVQVLFDHMEGVTLLMAGLMY
                     GGGLRLMECVRLRVMDIDFGRRQIMVRDGKGQKDRMTMLPERFSVVLKEHLARVRTVY
                     DDDRTRGVPGVYMWPVLARKYPKGALEWKWQYVFPAKSLSVDPQSAIVRRHHINENLV
                     QKAVKEAAGRAGINKRVSCHTLRHSFATHLLEARYDIRTVQELLGHADVSTTMIYTRV
                     LNRPKLSVVSPVDF"
BASE COUNT         2826 a         2608 c         2486 g         2852 t
ORIGIN      
        1 gaaactctcg gggggtaaga tcaaactctc ggtcattggc agctaccgtg ccggaggaga
       61 agcagctatg gttgacaaga tgggcgttgg tggcatagat ggagcgctca tgaccattgt
      121 aggactctca aaaataaaca aagatgcggc aggattgagt aacatcccga tgatctacaa
      181 gtctttggac gaggtagact ttgttgagga gaaattcggg gcagaactct caaaaacaat
      241 ggaacaaaag ggatttatcg tacttttctg gactgacata gggtggatca ggcatttctc
      301 agtccacccc attatgtatc cccatgactt aaagaagatg aagatgttta cttgggcagg
      361 aagtcccgaa caggtagaca taatgaaaga gtggggaaca aatccggtct ctcttgaacc
      421 atccgatatc ctctcgtctc tctcaacagg catgatcaac gtggtatcgg caaccccttt
      481 ttcggcgaat gccggccagt acgcaagggt tacaaaatac atgctggaga tcaactgggg
      541 cccgttagtc ggtggggcag ttatcaggaa acaagtatgg gaaaaaattc cggcaggcct
      601 gagggaggaa tttctcaagg tagctgctga aaccgggagg gaaattaagg aggcaggaag
      661 aaaagagagc gatgatgccg tcgaggtaat gagaaagaaa caaggtctgc atgtagtggt
      721 tccatcgcca caaattaatg aagaatggag acaagccgtg agggctgtct acccaaaaat
      781 ccgtagctca atggtaactg cggagagttt cgacagggta gagagtctct taagggggta
      841 ccatggaaaa agtactgcaa acaaatagtt tagattcctt aggcttcagg cggaggcctg
      901 gtacctgctt gctggcgcgg ctttgcaggg gaaaggaaag aaggtcgcta tcatcaaccg
      961 cagcaaacat cttcgagggt ctcgaaaatg gattgattgt tatggcttta gggctgatga
     1021 tggccctacc ccttgccgag agccttcttc ggagagtttt tggagttggg atcacgggct
     1081 cagcagtttt cgtgcaacac ctcgtgttgt atgtcggcat gctgggaagc gtcattgcag
     1141 cccgtaaggg gcgccttctc tcgatctcct ttgttacaac atatcttcag gggaaatgga
     1201 aaacagctgc ttcagtctat gcaaacttat tcgccgttgt tataacctgt tacctagtta
     1261 ttgccagcat acaattcatt cttgcgaaga aaatagttgg tgacaccctg atccacggtg
     1321 ttcccctctg gcccatcctg ttaattctgc ccttcggttt tgcactcgtt gcgtgccgta
     1381 cattccttca ggtttgttca tcctggacga tcagatgtgt tgcatttctt attatcactg
     1441 ctatgcttag cataggtact gtggtgtcgg ccaagagtct ggttctaccg ggcattgtcg
     1501 gacttctcct tgcttcagtc cttggagcac caatcttcac ggtactcggt ggtgcagcca
     1561 tgatcctctt ctggggaaac gggcaaccaa tcgccatggt acccatcaac cattaccgtc
     1621 tcactgtcga cgcgactctg ccggttatcc cccttttcac cctcgttggt tacttccttg
     1681 cagaaagtgg tgcagcacag aggcttcttg caatcttcca aacgatcttt tcaaagatgc
     1741 ggggcggggc ggttattatt accactgttg tttgcgcact attcaccgct tttacaggcg
     1801 cttcaggggt taccattctt gccctgggag gtctcctcat gcctattctc atttcatcac
     1861 gatattcgca gcgaaatacc ctcggcctcc tgaccagcgc cggttccctt ggccttcttt
     1921 ttccaccaag tcttcctcca atcctctatg ctgtagtggc aacaacaatc ggcagcgaga
     1981 taacaatcgg cagtattttc ctggcaggtc ttataccagg ggtcctgctt cttgtgctca
     2041 cctggtggtg gggcatacga tctgaccctt caacctatat tcaggaagca ttatcgggaa
     2101 ctaaagcttt aaaagcagta ttacaggcca aatgggagct tcttcttccc gttgtggccc
     2161 tcgtatctct tttttccgga attgccactc ctgtagaggc tgccgctttc acagctctct
     2221 atgcactcat tattgaggtt tgcgtatatc gtgacctgaa gtttttccgt gatgttccaa
     2281 gggtcatggt ggagtgtggt cttcttgttg gcggtgttct tattattctt ggtgttgcca
     2341 tgggattcac cgacttcctt ttcaccgtca gcgttccgga aaggcttgtg gaatgggcaa
     2401 aaaattccat tcattccaag tggctttttc tcatcgcctt gaacattttt ctccttgttg
     2461 ttggctgcct tatggacatc tattcggcca ttattgtggt tgcaccattg ataatccctt
     2521 tgggtagaga gtttggcatt gaccccttgc atctggcaat tatattcctg gccaatatgg
     2581 aactaggctt tcttacgccg ccggtaggaa tgaatctttt cctgtctgcg taccggttcg
     2641 atgttccaat gtctgaggta atccgatcaa tcctgccaat acttttggtt ctccttggag
     2701 gagtgttact catctcttac gtgccatttt tgacaactgc cctacctcat ctctttcaag
     2761 ctgttggaag gtgaaagtga aagactacaa gttaactact atggaggtgt cccaatgtac
     2821 agaataggac tcgatatcgg ctcggtaagc gtaaatgtca tagtcatgaa tgaagagaac
     2881 acgattgtaa gcagtcgtta cattcgccac atggggaggc cttttcaggc gggcaaaaaa
     2941 gccctggaaa agatatctga aaatttcgag gtcgattttg tatctaccac cggaaccggg
     3001 gccagggatt ttgccgcgct tatcggtgca tcctttgtca acgagattgt tgcccttgcc
     3061 aaggcattta atcacctcta tcccaacgtt cgtagtgtga tagacatcgg gggagaagat
     3121 tcaaagctta tccttttcga gcaggatgga caaaatgaaa ggattcgcgt caaggatttt
     3181 tcaatgaatg ctctctgtgc agcgggtaca ggatcgtttc tagatcagca ggcatcccgc
     3241 cttctattta ccatcgagga gttttcggag gttgccctaa agtctacaaa ccccccgagg
     3301 attgccggga gatgtacggt ttttgcgaaa tccgacatga tccatctcca gcagatagca
     3361 acgccagatt atgaaatagt ggccgggctt tgctacgccc tggcaagaaa cttcaaggga
     3421 aacatcgcca aaggaagaga catcgctgcg ccggttgcct ttgtgggtgg cgtagcggct
     3481 aatgctgcaa tgcgccgtgc cttgatggac gtcttttctc tcaatgatac gcaatttttt
     3541 gtaccccagc actttacctc catgggtgcc gcaggtgccc tttttgccgt tcttgatgaa
     3601 ccgtcattac gtaaccgcta ccttggtctc gggagcctca atgactgtct catgaataaa
     3661 aaagtccggg ctgcccacga gccgctggtc atctctaatg aaaacctgcg cgtctcctac
     3721 gacatgaaac ctgtagacgg aaaaacagag gtatatctag gtgttgacgt gggatccatc
     3781 agcacaaatc tcgttgtaat cgacaaggag aagaatgttc ttgcaaaacg ctaccttatg
     3841 acggaaggac gccccctcga ggctgtaaag aaagggctcg ccgagattgc agacgaggta
     3901 ggcagacagg ttgaaatcct cggcgcaggt acaaccggat caggccgcta tctgactgcc
     3961 gacttcatcg gcggcgacat agtgaggaac gagatcactg cccaggctga ggcagccatc
     4021 catattgatc ctgaagtgga tactgtgttc gagatcggtg gacaggactc caaatacata
     4081 agcattgaca atggggttat agtcgatttt gagatgaaca aggcatgcgc agcaggcaca
     4141 ggatcattcc ttgaagaaca ggcagaacgt cttggaatct caattaaaga ggaatttgga
     4201 aacctcgccc tatcggccaa aaagccggtg aaaatgggag aaaggtgcac ggtctttatc
     4261 gaatcggacc tcgttcacca ccagcagatg ggtgaaggca cagacgatat agttagcggt
     4321 ctttcgtatt ccattgttgc aaattattta aacaaggtag taggtgacag aaaagtagga
     4381 gataagattt tctttcaggg tggcactgct ttcaataaag gagttatcgc tgccttcgag
     4441 aagacagtcg gcaaaccgat aacagtgcct tcccaccatg acgttacagg cgctatcggc
     4501 gtggccctgc tcgccatgaa agaaaagagc tgggaccaca gtaactttaa gggttttgat
     4561 cttagcaagc ggccctattc tatcgaaaca ttcgaatgta aagggtgcga gaacctctgc
     4621 gaaatcagaa aagtgaccgt cgaaaacgaa agccctcttt tctatggaag caggtgcgag
     4681 aagtatgacg tggtaagacg cagtgagaaa cgaaacatgc ccgacctttt taaagtgcgg
     4741 gacgaactcc tccactccac atatgaccga acgattgacg gcgaacctat cggtgtccca
     4801 aagatcctga atatgtggga attcatgcca ttctggaagg cctttcttac agaactcgga
     4861 ttttccccgg ttttctctga ccctaccaac aagaagacga tacgcgaagg tgtcgagagc
     4921 attatcgtgg aaacatgttt tcccatcaaa ctcacccatg gccacatact caatatcatc
     4981 aataagggca taaaacggat atttatcccc agcgtaataa acctgagagg gacttcaaaa
     5041 aatgctccta attccttcgc ctgtccctat gcgcagtcca tcccttacag tataagggca
     5101 tctatcgatt ttgacgccca cggtgttcag atggatacgc caattgtcta ttttggcaaa
     5161 aacaaatcag aaatactcga ctgtcttgtt tcctacggga aagagataaa tcgttccaag
     5221 atagaggtga aaaaggcttt aaccactgcc ttcgaggttc aggaaacatt ctacagaaaa
     5281 atagctcaag ctggaacaga tgcactttcg gctttaaatg acaatgacaa ggcaatggtg
     5341 ataatcggca ggccatacaa cagcgcagat cccggggcaa atctcaacat ccatagaaag
     5401 cttatggctc tgggtgttca gccgctacct atggacatga tgcctatcgt tgaaaatgcc
     5461 gtcattgatg gagatctgga aaacatgtat tggggttacg gccagaagat actgcgcacg
     5521 gcaagaagca taaaggattt gccaaatgta tatggcatat atttaaccag cttcggctgc
     5581 gggccggatt cttttataac tcacttcttt aagaggatca tcaggggcaa accatttctc
     5641 cagttggaga ttgatgaaca cagcgcagat gccggaatcg ttaccaggct cgaggccttc
     5701 cttgacagca taaaaaatgc aaagcaaaac agcacaatct caaaatccag actaccgcac
     5761 gtcgccacaa aatcgcatcg acgaataatt tatatcccga atatgtcaga tcataccatt
     5821 gccttcaaga gcgcctttcg agcctgtggt caagaggctg aagtcatgga tgagcctgac
     5881 gatcagtcag tttactgggg aaggaaatat acgtccggaa aagaatgtta cccatgtatc
     5941 ctgactacag gggacatggt caaaaaggtc aagcagtccg acttcgatcc tgaacgaagc
     6001 gccttcctca tgccatcagg aaatggtccg tgccgtttcg gccaatacca cagatttcac
     6061 cgtatgattc tcgatgagat gggattcgaa aatgtacccg tctatgcacc caaccaggac
     6121 gacaagctat acaaggattt caatattgta ggaaaccgtt tttcaaagct gggctggcag
     6181 gccattgtag caactgacat gcttatcaag atgctccatg agatacggcc catggagata
     6241 ataagaggag atgctgaacg ggtgtactac aaatcaatcg agtcaatcgc ttactcaatt
     6301 gaaaagggca ataacgggat tgtcaatgca cttcatgatt cgatcaatcg tttcctccaa
     6361 atcaaaagga atgaaatccc aaggccgatc gtaggggtcg tgggcgaagt ttacgtcaga
     6421 tgtaacaggt tcagtaataa cgatgttatt aaaaaagtgg aagaactcgg gggacaagcc
     6481 tggtttgccc cgttaacaga atggatctcc tatatcaact tcagcaacaa acgacgtaaa
     6541 agtgtaagga agagaagcat aactcatgta ttaaggacat ctcttaccga atacattcag
     6601 ggcaaggaag agcgaagact ggaagaacct tttatgggac tcatccagta tggagaagag
     6661 ccttcggtga acagcgtaat tgacaaagca agtctctata tcgatccaag cttcgaaggt
     6721 gaggctattc tgagtgtggg caaggccata gattttattg acaagggtgt ttcaggtatt
     6781 atcaatgtca tgccttttac ctgcatgcca ggaaccataa cgagtgccgt cttgaggctc
     6841 gttcaaaaga aatataacat tcccatcatc aatgtcgctt acgatggtca aggtctcaca
     6901 aacattgata cacggctgga ggcgttcatg taccaggtac gcgagcattt tgcggattct
     6961 gataagatgt ctggcagtat gagcttacag ctatgagctg attagtgaat cctcggccat
     7021 ggctcaggcg caggctcctg ccttctgttt ggtactgtct ccggcctctg gtcaggccgc
     7081 gggggagaac ctccatcaga cctgaacccc ctgaaccact cgaggatagg agaaaaaacc
     7141 gaaggcgccg caaagtcatc aggcgggtat cgcctgagcg cagtacccat aaagctcatc
     7201 cagattggta aacataccct tgccccactc tctttctctc ccagggatgt tctcgtatca
     7261 aaaccaaccc ataccccggt gattacatgg ggcgaatacc cgatgaaaag tgcatcgtaa
     7321 taattgctcg ttgtgcctgt tttgcctgcc accggatagc caagccgggc agcgcctttt
     7381 gctgtgccat attctgttgg tcctttcaga aggacattca tattgtatgc aatatcctct
     7441 gataaaaccc gcactttttc ttcatggttt tccatcaatg tatttcctgt ggaatcaaca
     7501 accttcaaaa caccaatagg ctttacacgc aaaccaccat tggcaaacac agcatatccc
     7561 ttaaccaggt caagaagggt caggtttgat gtcccaagcg ctatagcaag ctcgtgagga
     7621 agatctgcat ctatgcccag atcagtcatc acctctttga cagcattcac acctacttct
     7681 tccaacagac gcacggtggc ggcatttttc gaataggcaa ctgcgtcccg cattgaaatc
     7741 tgacccacat acttgccatc ataattcctt ggtgtccatg cctttcccat acccatagaa
     7801 tattcctttg gttcatccac aagcatggta tcgatggaat agcctttttt aagggctgcc
     7861 aggtaaataa atggtttgaa tgcactgccc gactgaattt tagcagctat cgcccggttg
     7921 tatgggctct gttcaaaatc ccttccaccg accatggcaa ggatgtatcc tgtctgaaca
     7981 tccagactaa tgagcgcccc ttcaactttc actgttttga tagggtgaaa cacatggatc
     8041 ttttctccgt caatcccatc atatgtggcc tttatgatat cgcctggttt gtatggaaag
     8101 gcatccattt tcaaaatgcc tttttcattg cccgcaaaaa ctgcataacc attgtcgagc
     8161 ttatccgata caagaagatt gtattgttta ccggccttca aacctacctg tttgtaatcc
     8221 cgttctctgg cctttttata attattccac ttctttctat cgatatggct caccacactg
     8281 taagcacctt ttctttgctc atacaggttt aaacctcttt taacagcgtc ttctgcaagc
     8341 ctttgcaacc ctgcatctgc tgtggtataa attgataatt ttcgtgtagc aagaacaccc
     8401 ttgccaatct tctcctcaac agatttgagt acgtaatcct taaaataact atctgtaaat
     8461 accccgttgt tttcgcggat attcaccttc tccttgagta tctcgttcct gcgtcgctca
     8521 tcgatgaact tcttttcagc catctgccca ataacatatt cctgacggac gcgagctgca
     8581 ccgggatatt tttttggcgt gtaccgcgaa ggcgcctgaa ctatccctgc aagcagagca
     8641 gcctccgccc ttgtcacgtt ccacacatgt tttccaaaat atatctgtga tgctgcctca
     8701 accccataga caccgtgtcc catgtataca ttattcagat agaggtttaa aatctctttc
     8761 ttcgtcaaag acccttccag ccggtacgaa agaatcgcct ccctggtctt cctcaggata
     8821 ctcctttcag ggccgagcaa aagcgatttg atcacctgct gggtgatggt gctcccgccc
     8881 tggacaaccc ttcctgatgt aatatttttc cagagcgccc tcaccacact ttgaaaatca
     8941 attgcgccgt gtttaaaaaa ctctgcatct tccgcagcga gaaatgcatc ctttacatgc
     9001 tgaggtatct tgttataatc gatgaatatt ctgttatcag gaacaatcag gtatattaat
     9061 tctccattcg caccataaat actgctcaca ggcttatttt caaggcgctt gagtaccttc
     9121 accgagggga tatctttcat aatatatatg tagaaacccg tggcaagaag gataactgag
     9181 aacgccacca tggacaccat gtatattaat atcttccgtt tcaaaggctt atttctccgt
     9241 ttatgtgcca ttaatctggt gtatccattt taatataata attttgcctc ttcaggaatg
     9301 ttttctcctg acgtgtcatc acataattta attctattgt gttccgatga ttagctggct
     9361 gctgacagct aacggctggc ctgccggttt tacataatcg cgcatgtcac aattgccttg
     9421 gcaactaact ggccgtcgct gtttttgata tccacgtcag ccaacgcaaa tttcttgcct
     9481 ttgtgaacta ttcttgcctc tgcaaatatg gcggtatctt taacaggcaa aagatagtta
     9541 atcttcatct caagggtgag ggctttctgc tcatctttca acattgttaa tactgcattt
     9601 ataccggcgg aatctgccag gctcgccaca gctccgccgt gaagataacc gaaggggtga
     9661 gtcaggcact ccataaagtt tagccgaagt ttcgcaaaac cttcccttgc ttccacaatg
     9721 gtgatgccaa gatgtttata atagggagac tgctcagcaa gctgtaatgg aagatccaat
     9781 ttcatagcaa tttcactaaa atctatcgca aaaagaaaat ttaatcaaat ggcagcggaa
     9841 ggcccctaaa catcaaaagt caacaggact cacgaccgaa agttttggcc tgttcaagac
     9901 gcgggtatag atcatagtgg tggagacatc tgcgtgtccc agcagttcct ggaccgtgcg
     9961 gatgtcgtag cgggcttcca aaagatgagt ggcaaaggaa tgacggaggg tatggcaact
    10021 gactctcttg ttgattcctg ccctacccgc cgcctcctta acagcttttt gcacaaggtt
    10081 ttcattgatg tgatggcggc gtactatggc gctctgcggg tcaacagaga ggctttttgc
    10141 gggaaataca tactgccact tccattccag cgcacccttt ggatatttac gggccagaac
    10201 aggccacatg tagacaccgg gaacgcccct ggttcggtca tcatcataca cagtcctcac
    10261 cctggcaagg tgttctttca aaacgaccga aaagcgttca ggcagcattg tcattctatc
    10321 cttctggccc ttcccgtccc gtaccatgat ctggcggcgt ccaaaatcaa tatccatgac
    10381 tcgaagcctc acgcattcca taaggcgcaa cccacccccg tacatcaggc cggccataag
    10441 cagcgtaaca ccttccatat gatcaaacag aacctggact tcctctcttg tcatcacctc
    10501 aggcagcctt tgaggacgtt tggccctgac aaaggtctcc aggtctccaa agggtttgtt
    10561 gagaacatta gtatacagaa ataccagggc gttcagggcc tggttctgag tgctggctgc
    10621 caccgccctt tcaacggcaa gatattcaag atattctctc actgctttcg tcgcgtcaag
    10681 tttctttaaa tcagtatagt catgaaacgc aatgaagcgt cgcacccaat caacgtagga
    10741 ggattcagtg cggatcgagt agtgacggct cc
//
LOCUS       DAOOMC010000097        10699 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdus sp. isolate AMR_MDS_2685
            NODE_7004_length_10699_cov_5.845077, whole genome shotgun sequence.
ACCESSION   DAOOMC010000097 DAOOMC010000000
VERSION     DAOOMC010000097.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864760
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdus sp. (wastewater metagenome)
  ORGANISM  Syntrophorhabdus sp.
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae; Syntrophorhabdus.
REFERENCE   1  (bases 1 to 10699)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10699)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,729
            Genes (coding)                    :: 2,708
            CDSs (with protein)               :: 2,708
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 7 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10699
                     /organism="Syntrophorhabdus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7004_length_10699_cov_5.845077"
                     /isolate="AMR_MDS_2685"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2699756"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2171)
                     /locus_tag="PK125_10030"
     CDS             complement(<1..2171)
                     /locus_tag="PK125_10030"
                     /inference="COORDINATES: protein motif:HMM:NF037959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HPB38480.1"
                     /translation="MGAHTNFIFAVLITGFSGIVAQTILLREMLIIFSGNEFSIGIII
                     GSWVVWEAIGAISGGKISDRIKEHGTLLVWLMILFAVSFFGSIYLARVVKILMGVSPD
                     VGLGIVETLYYSLIILLPTGFFHGMLFTVACSMYSRLTKGASSSIGKVYFYEMLGTIV
                     GGVLVSYIFIPVFNSFKIALGVALLNALICLIMLLFSGMARKTLALVSGFAVLVISIV
                     FLAGNGAERVHLDSIAKQWQGKKVISYENTLYQNIVVTRNQDQLTFYTDGIPVMTTPV
                     PDITFVEEFSHISLLAHQNPGSVLVLGGGAGGVVHEILKHPSVTTVDYVEIDPQFLIT
                     VKKFSTPLAHQDLTDKRVAQHYMDGRVFIKKTTRKYDAILLGIAPPLTLQANRFFTQE
                     FFKNIRSILNERGILSFYFTGSTSYYNHELRYMNACMYGTVKSVFPNIFVVPGDMNLF
                     IASVDSDVAGISSPLLSRRMTERGIKANLVSETHLDYLFQENRRQWYISLVDFQATPL
                     NKDFSPKGLYYNILFNQLLFTPYLKPFFDRIVMLNAYTVVAIFGGLFLVFLGLQKKYQ
                     TISLPFAITTTGFASMVLQLVLLFGFQIIYGYVFYEIGILITAFMAGLAIGGIMTANW
                     PSHGRRKLGIFMKIESGIILLSVVLIFFFRYLEAGIQIEPILLRIVFLFLLLVSGLLT
                     GMEFPLANRLYIQQTDYFSFRRNEIGKTVGLLYCVDLLGGWV"
     gene            2327..2420
                     /locus_tag="PK125_10035"
     tRNA            2327..2420
                     /locus_tag="PK125_10035"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2361..2363,aa:Ser,seq:gga)
     gene            2437..2534
                     /gene="ffs"
                     /locus_tag="PK125_10040"
     ncRNA           2437..2534
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="PK125_10040"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            2691..4181
                     /gene="dnaX"
                     /locus_tag="PK125_10045"
     CDS             2691..4181
                     /gene="dnaX"
                     /locus_tag="PK125_10045"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004046.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HPB38481.1"
                     /translation="MSYTVIARRWRPKKFDDVIGQPHVVKTMKNSVKYGRLSHAYLFG
                     GPRGVGKTSLARILAKAINCLNITADEPCGTCENCVAIDQGSFPDIIEIDAASTRGID
                     DIRELRDTVRYMPLMGKYKVYILDESHQLTGPAKDAFLKTLEEPPNHSIFILATTEAQ
                     KISPTILSRCQRFDFRRISEVEIVKQLKRICDEEGITYDEEVFPYIAVEADGSLRDAE
                     SILDQVISFSGEHLAYKDVLSIIGIIENEILFTLIRSILEENLKMGLEIIEKSLNEGN
                     DVHQIYRGLVSMLRNMMILKICEERPSFLHLADEEYQRLSGIIEQIEYYEIQNMLNYL
                     LTAEDLLKGLFPKIALEILYINLYNISKLRDIEKIIDTTEKPEFLTEKREFQNEETLP
                     APYKEPEAGKFGQDAHGFVEYLKKKMPSVGNMMDSHEVKVEGHMFVVLADKQSQTLFT
                     HRGEEVKKHLTEFFGRPMNYVVRDAGDTKRNTLDEYVKEAQSLFNR"
     gene            4192..4506
                     /locus_tag="PK125_10050"
     CDS             4192..4506
                     /locus_tag="PK125_10050"
                     /inference="COORDINATES: protein motif:HMM:TIGR00103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HPB38482.1"
                     /translation="MSKQLGQLMQQAKKMQERFQKMQEEVAVKTVEAQSGGGMVSCVV
                     NGKQELVSLKISEEIWEEKDRELLEDLIVAAINDGLSKSKDMLQEEMSKITGGMPLPF
                     NL"
     gene            4515..5111
                     /gene="recR"
                     /locus_tag="PK125_10055"
     CDS             4515..5111
                     /gene="recR"
                     /locus_tag="PK125_10055"
                     /inference="COORDINATES: protein motif:HMM:TIGR00615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HPB38483.1"
                     /translation="MYYPAPIERLIENLSKLPGIGRKTATRLAFYLLNTKESYVSELS
                     NSLIDIKEKIKLCSTCFNITDVDPCPICRDEKRNSATVCVVEESSHMMVIESANPGYY
                     RYHILHGVINPIEGIGPDEVRIKELRDRVQREGIREIIIATNPNIEGNTTAHYIAETL
                     QDLDLKITRIASGIPIGGDIVYIDPLTIKSSLDNRRNL"
     gene            complement(5114..5401)
                     /locus_tag="PK125_10060"
     CDS             complement(5114..5401)
                     /locus_tag="PK125_10060"
                     /inference="COORDINATES: protein motif:HMM:NF014636.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HPB38484.1"
                     /translation="MKVKVHSILQLKEILGGKDVEIIVPDGTNVSGLLAGMVEIWGDS
                     LSPHIFKPQSDEVLPYIRLMINGTAIQSLDGMETILNDGDEVLLIPLAAGG"
     gene            complement(5427..7301)
                     /locus_tag="PK125_10065"
     CDS             complement(5427..7301)
                     /locus_tag="PK125_10065"
                     /inference="COORDINATES: protein motif:HMM:NF013481.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase family
                     protein"
                     /protein_id="HPB38485.1"
                     /translation="MYSGGYTGKILRVNLTSKTAQEEKLPLAVAKDFIGGAGLGMKYL
                     FDEVKPGTDALGPDNKLIFAPGPFTGTSIPCASRMAVVGKSPLTGAVGNALTGGYFPA
                     ELKFAGWDAVIVEGKAEKPTWVSIKDSSVRFHDAGSLWGTMTFDCQQMIKDNLNDQNT
                     RIICIGPAGERLSKIACIINERRAAGRKGLGAVMGSKNLKAIAVRGTGKVAITSEEKY
                     RAARSEMLKAFKESPVLYSAFSPAGTPMVVDLTGALGILSAKNWSATGEFVPVDQLGL
                     DAQNTRKIGKEHCYDCPVGCSQMKLAKTGPYAGILTEGPEFETIYSFGTQTGVVNLDA
                     VIAADRLADELGLDSMSAGVTIGFAMELFERGILTPADTGGIDLKFGNDQAMMMVLRM
                     MAFREGIGDLLADGTRVAAMKIGKGSEKYAMHIKGLELPAYDVRGAKAHGLNYATAYT
                     GADHNRGYAFQEIFGIPVPWEVDRLSIEGKGKLTKWNQDVRAATCDCATMCAFMLDTA
                     LPATSTQNTSALLEAVTGLIFTPDEIQKVGERLNNLAKVFNIREGFTRTDDTFPERLM
                     TEPLKDGASKGQVITKDDLKKMLDEYYTERGWDTQTGVPTRAKLEELGLSTAADLLGV
                     "
     gene            complement(7393..8157)
                     /locus_tag="PK125_10070"
     CDS             complement(7393..8157)
                     /locus_tag="PK125_10070"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HPB38486.1"
                     /translation="MRLKDKIAIITGASQGIGAAFAAGFAKEGAKIVIADISDGAKTV
                     KKVADAGSEALFVKTDVSKEPECMAMAKAAYDKFGSIDILINNAAVFANIVLKPFTEL
                     SSEEFKKVIEINTSGVFHCIKAVFPYMKEKGGKIVNISSASILEGIPGMPHYVASKGA
                     VMALTRCMARELSDFMINVNTIAPGFTHSEGGDQFDRGKALPLPPLDELQLQGRCIKR
                     PSMPEDLVGLALFLSTNDSAFITGQMIVHDGGLSLH"
     gene            complement(8320..9540)
                     /locus_tag="PK125_10075"
     CDS             complement(8320..9540)
                     /locus_tag="PK125_10075"
                     /inference="COORDINATES: protein motif:HMM:NF024850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HPB38487.1"
                     /translation="MKSKRIFWWFGLFCSIPIICLVFSNFAMAAGKEILIGAPVHMTG
                     PLAPDGLELKWAYEQAVADWNKKGGIFIKSANKKLPIRLVFADDESEPAKVAGAIEKL
                     IKVDKVDLLLGTHSATMSVPAINTAEKYQMVYHCTTHWPEMFRDQKAKWASVMFFSAV
                     GASEVPFIIWETLLKKEDRPKNVALLMEDSPDGQAFGGGFRSHAKTHGVTFAVDEPWA
                     IGAKDYSSMILKMKAKNVDAILLYGAPTDAITLVRQIKEAKLNVKYMHGWKGTWAVEF
                     AQALGKDANYVICDGFWSEEFPYPLCKELGKRYTDKFKKRSVSIGAFYGTAQVLFMAI
                     ERAKSYDKTAVRDALRNAEYKGTVMGDLKFDEIGLALFPSTANQWWDGKQMLFYPPVK
                     GGYQLKLAPEWGKR"
     gene            complement(9821..>10699)
                     /locus_tag="PK125_10080"
     CDS             complement(9821..>10699)
                     /locus_tag="PK125_10080"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HPB38488.1"
                     /translation="FVSDSEKSYLVYFLFLTFCHIVLTQGWNLVAGYTGQVSLGTHAF
                     FALGAYTTAMIWRADLFSWENYYFDPVTMILSGLVPVIFALAIGIPLLSKLHGDYFAL
                     GTLGFGEILKVVCIKGGQFTGGPIGIMLPSSVYTTMRPHYWTALFLAFLATGVVYFMT
                     KSRVGLALVAISEDETAASSSGIYILKYKVLAFAISAFLSGLCGSLYAYYLFQVSPEG
                     FLILRWTLYPVLMCVIGGSGTVVGPILGSLLMTAVFTVTSIYLPGIHPIFSGIIIILV
                     MLFMPNGIIRLWKKGV"
BASE COUNT         3002 a         2509 c         2382 g         2806 t
ORIGIN      
        1 acccagccgc ccaaaagatc aacacaatag agaagaccaa ctgtcttgcc aatttcattg
       61 cggcgaaaac tgaagtagtc ggtctgttga atatataacc tgtttgcgag agggaactcc
      121 attcctgtga gaagtcctga aaccaggaga aggaagagga agacgatgcg cagcaaaata
      181 ggttcgattt gaatgcctgc ctcaagatat cggaagaaaa agataaggac gacagaaaga
      241 aggataatgc cagactctat tttcatgaat attcccagtt ttcttcttcc atgacttggc
      301 caatttgctg tcataatgcc gccaattgca agaccagcca taaaagcggt tataagaatt
      361 ccaatttcgt aaaaaacata cccatatatg atctgaaaac caaacaaaag gacaagctgc
      421 aaaaccatgg aggcaaatcc tgttgttgtt atggcgaagg gcaggcttat ggtttgatat
      481 tttttctgga gaccaagaaa gacaaggaat agtccaccaa aaatagcaac cacagtatag
      541 gcgttcagca taacaattct atcaaaaaaa ggtttcaggt agggggtgaa aagaagctgg
      601 ttaaaaagga tattataata taggccttta ggtgagaaat ccttgttgag aggtgttgct
      661 tgaaaatcga ccagtgaaat ataccattgt ctgcggtttt cctgaaaaag gtaatccaga
      721 tgagtctcac tgacaaggtt cgccttaatg cctcgttctg tcattcttct cgacagaagc
      781 ggtgacgata tgcctgcaac atcactgtcc accgaggcaa taaagaggtt catatctcct
      841 ggcaccacaa agatattagg aaagacgctt ttgactgttc catacatgca tgcgttcatg
      901 tatctcaatt cgtggttgta gtatgacgtt gatcctgtga aataaaacga caggatgccc
      961 cgttcattca ggatgcttcg gatgttttta aaaaattcct gagtaaaaaa tctgtttgcc
     1021 tgcaaggtta acgggggcgc aatgccaagt agaattgcgt catacttgcg ggtggttttt
     1081 tttatgaata cccttccatc catatagtgc tgggcaaccc ttttgtcagt caggtcctga
     1141 tgtgccagag gcgttgaaaa tttcttcacc gtaatcagga attgggggtc aatctcaaca
     1201 taatcaacgg tcgttacgct tggatgtttg aggatttcat gaacgacccc gccggctcct
     1261 ccgcccaaaa ccagcacgga cccgggattt tgatgagcga gcagggaaat gtgggagaat
     1321 tcctcaacaa aggttatgtc aggtacggga gttgtcatca cagggatgcc atctgtataa
     1381 aacgtcaact ggtcctggtt ccttgtgaca acgatgttct gataaagggt attttcgtaa
     1441 gagatgactt ttttaccctg ccattgtttt gcaatagaat caagatgaac tctttctgcc
     1501 ccatttcctg caagaaaaac aatagatatg acaagaactg caaaccctga aaccagagcg
     1561 agggtttttc ttgccatacc tgagaagaga agcattataa gacagatcag tgcgttcagt
     1621 aaggctacgc caagagctat cttaaaggaa ttaaaaacag ggataaaaat atagctgacc
     1681 aacacaccac cgacgattgt gccgagcatt tcgtagaaat atactttacc aatggacgaa
     1741 gaagcgcctt tggtaagtct gctatacatg ctgcaagcta cagtgaagag catgccatgg
     1801 aaaaaccctg ttggtaagag gatgatcaaa gaatagtaaa gcgtttcaac aattccgaga
     1861 ccaacgtccg gtgagacgcc cataagtatc tttactacac gagccaggta aatacttcca
     1921 aagaaagaga cagcgaagag gatcattagc cataccagga gggtaccgtg ttcttttatt
     1981 ctatccgaaa ttttgcctcc tgaaattgcc cctattgctt cccataccac ccatgacccg
     2041 ataataatac cgatggagaa ctcattccct gaaaaaatga tgagcatttc cctgagcagg
     2101 attgtctgtg caacaatccc gctgaaaccg gtgattagaa cagcaaaaat gaaatttgta
     2161 tgggccccca tagacactga tatagcttat aagggagaca ggtgaagagt caacaaaatc
     2221 ccgccttcgc tcccatcttc tcaccttcat tgccgtcttg ctcctgctcc tccctctccc
     2281 gaattgcctt gagtgacatg gtcaagtatg gtataacact ctgtccggag agatgtccga
     2341 gaggctgaag gagcacgatt ggaaatcgtg tgtcccgtca caaaacggga ccgagggttc
     2401 aaatccctct ctctccgcca gatacgaaaa tagaaaagcc aggccctgcg caacgtgaag
     2461 gttatgaacc ccgtcaggtt cggaagaaag cagcggtagt agctaatccg tgtgccgcag
     2521 ccagcctggc ccctatttta gctgtaaatt gctgacggtg atttgtaaac agcgagcaga
     2581 aagtagtttt tgggtaatac gatgaacgaa ttcaattcac gaccctttgt ctttaattca
     2641 aaagccaaaa gtcaaaagcc aaaattaaaa gctggagaga ttttttccgc atgagttaca
     2701 ctgttattgc ccgaagatgg aggccaaaaa agtttgacga tgtgattgga cagccgcacg
     2761 tggtcaagac catgaagaat tctgtaaaat atggccggct ctctcatgca tatcttttcg
     2821 gtggcccccg aggcgtagga aaaacttccc ttgcacgaat ccttgcgaaa gcaatcaatt
     2881 gcctcaatat aaccgcagac gagccatgcg ggacctgtga aaattgcgtt gccatcgacc
     2941 aggggagttt tcctgacatt attgagattg atgctgcatc tactcgtggc attgatgata
     3001 tcagggaact gagggatact gtaaggtata tgcccttgat ggggaagtac aaggtctaca
     3061 tccttgatga atctcatcag ctcacaggtc ctgccaaaga tgccttttta aagacactcg
     3121 aggagcctcc gaatcacagc atctttatcc ttgccacaac agaggcgcaa aaaatatcgc
     3181 ccaccatact gtcccggtgc cagcgatttg acttcaggag aatttcagaa gtagagattg
     3241 ttaaacaatt gaaacgtata tgcgatgaag aagggattac ttacgacgag gaggtattcc
     3301 cgtatattgc tgtcgaagct gacggaagcc tcagagatgc agaatcaatc ctggaccagg
     3361 ttatttcatt ctcgggagag catttagcat acaaggatgt tttgagcatt atcggaatta
     3421 ttgaaaacga gatccttttt actcttatca ggtcaattct ggaagagaat cttaaaatgg
     3481 ggcttgaaat aatcgagaag tccctcaatg aagggaatga tgttcatcag atatatcgtg
     3541 gccttgtttc catgctaaga aatatgatga ttttaaaaat ctgtgaagag agaccttcat
     3601 ttcttcatct tgctgacgaa gagtatcaga ggctttctgg cataatagag cagatcgagt
     3661 actatgagat ccagaatatg ctcaactatt tactcacggc cgaggatctc ctgaaagggc
     3721 tcttcccgaa gattgccctt gagatactgt atattaatct ttataacatt tcaaagctca
     3781 gggacatcga aaaaattatc gataccacag aaaagcctga atttctgaca gaaaagaggg
     3841 aatttcaaaa tgaagagact ctgcctgctc cctataagga accagaggcc gggaaatttg
     3901 gacaggatgc acatggtttt gtggaatacc tgaaaaagaa aatgccttcc gtggggaata
     3961 tgatggatag ccatgaagtc aaggtagaag gccatatgtt tgtggtcctg gcagacaaac
     4021 agtcacagac attgttcaca catcggggcg aggaagtcaa aaagcatctc acagagtttt
     4081 tcgggaggcc catgaattat gttgtccgtg atgctggtga tacaaagagg aatacccttg
     4141 atgagtatgt aaaggaagca cagagtctgt ttaaccgata aaggagggat aatgtcaaaa
     4201 caattgggtc aactcatgca acaggcaaaa aagatgcagg aaagatttca aaagatgcag
     4261 gaagaggttg ctgtcaaaac agttgaagcg caatccggtg gtggtatggt ctcctgtgtt
     4321 gtaaacggaa aacaagagct ggtatcgtta aaaatatccg aagaaatctg ggaagagaaa
     4381 gacagggaac tcctcgaaga cctcattgtt gcggctatta atgatggctt aagtaagtca
     4441 aaagatatgc ttcaggaaga aatgtccaag attaccggtg ggatgccgtt acccttcaat
     4501 ctttagtctc tgccatgtat tatcctgcgc caattgagcg ccttattgaa aacctttcta
     4561 agctgcccgg tattgggaga aagacggcaa cacgactcgc tttttatctc cttaacacaa
     4621 aagaaagcta tgtctcagag ctttcaaata gcctcattga tattaaggaa aagatcaagc
     4681 tgtgcagcac ctgtttcaat attactgatg ttgatccatg ccctatttgc agggatgaaa
     4741 agagaaacag tgcaactgtc tgtgtggtgg aagagtcctc tcatatgatg gttatcgaat
     4801 cagcgaaccc tggctactac cgttaccaca ttcttcacgg agttattaac cccatagagg
     4861 gtattggacc tgacgaggtg aggataaaag agttaagaga cagggttcag cgagagggta
     4921 tccgtgaaat cattattgcc acaaatccga acattgaggg caacacaaca gcgcattaca
     4981 ttgcagaaac cctccaggat cttgatctaa aaataacacg cattgcttcg ggcataccga
     5041 tcggtggtga cattgtatat attgatccac tgactatcaa gagttcactg gacaacaggc
     5101 gaaacctgta gatctacccc ccggctgcaa gtggaatcag taacacctca tccccatcat
     5161 tgagaatggt ctccattcca tccagggact gtatagcggt accgttaatc atcaggcgaa
     5221 tatagggaag aacctcgtca ctctgtggtt tgaaaatatg gggagaaaga ctgtctcccc
     5281 atatttcaac catacctgca agaaggccac tgacattggt gccatcagga acgatgattt
     5341 ccacatcctt tcctccgaga atctccttaa gttgcaggat cgagtgaact ttaaccttca
     5401 accttcatac ctacagattt agtctgctat acccccagga ggtcagcggc tgtactcaat
     5461 ccaagttctt ccaattttgc cctggtcggt acgcctgttt gtgtatccca acctctttca
     5521 gtgtagtatt catcgagcat cttttttaaa tcgtctttgg tgatgacctg gcctttcgat
     5581 gcaccgtctt tgaggggctc tgtcatgagc ctctcaggga atgtgtcgtc tgtccgtgtg
     5641 aagccctccc tgatgttaaa gaccttggca aggttattta gtctttcccc gaccttctga
     5701 atttcatcag gggtaaagat caatccggta actgcttcca aaagggcgga ggtgttctgg
     5761 gtggatgtgg caggcaatgc agtgtcgagc ataaaggcac acatggtagc acagtcacag
     5821 gtggctgccc tcacatcctg gttccatttt gtgagcttac ccttaccttc aatagataac
     5881 ctatccacct cccatgggac aggtattccg aagatttcct ggaatgcata gcctctgttg
     5941 tggtcagcac cggtgtacgc ggtggcataa ttcaagccat gcgctttagc tccccgaaca
     6001 tcataggcag gtaattcaag cccttttata tgcatggcgt atttttccga gccctttcca
     6061 atcttcatag ctgctaccct tgtaccgtca gctaaaaggt cgccgatacc ttcacgaaaa
     6121 gccatcatgc gaagcaccat catcattgcc tggtcattgc caaacttcag gtctatgcca
     6181 ccggtatcag caggcgtaag aatacccctt tcaaacagct ccatggcaaa acctatggtt
     6241 acccccgcag acatgctgtc aaggccgagt tcatcagcga ggcgatctgc agctatgact
     6301 gcatcaaggt tgacaacacc ggtttgtgta ccgaaagagt aaatagtctc aaattcaggc
     6361 ccttcagtca ggattcctgc ataaggtcct gtttttgcga gcttcatctg actgcatcca
     6421 acagggcagt catagcaatg ttcctttcct atcttgcgcg tattctgggc gtcgagtccg
     6481 agttggtcca ccgggacaaa ttctccagtt gcagaccaat tttttgcgga taagattccc
     6541 agagctccag tgagatcaac taccatggga gtgccggcag gagagaaagc agaatagaga
     6601 acagggcttt ctttaaaggc ctttaacatc tcagacctcg cagctctata tttttcctcg
     6661 gatgtgattg ccaccttccc tgttcccctg actgctatag ccttcaaatt ctttgagccc
     6721 atcactgctc caaggccttt cctgcctgca gcgcgtcttt cgttaataat acaggctatt
     6781 ttactgaggc gctcgccagc gggccctatg caaataatcc gcgtattctg gtcgttcagg
     6841 ttatctttaa tcatctgctg gcagtcaaag gtcatggttc cccataaact gccagcgtcg
     6901 tggaatcgaa cagaactgtc cttaatggat acccatgtgg gtttttcagc ctttccctcg
     6961 acgataacag catcccatcc ggcaaatttc aattctgccg gaaagtatcc acctgttagt
     7021 gcattgccta cagcaccggt aaggggagat tttcccacta ccgccatcct gcttgcgcag
     7081 ggtattgatg ttcctgtaaa tggtccagga gcaaagatca gtttattgtc cggtcccagg
     7141 gcatctgtac ctggttttac ttcatcaaag aggtatttca tgcccagtcc tgcaccgcca
     7201 ataaagtctt ttgccaccgc aagaggaagt ttttcttcct gtgccgtctt gctggtgagg
     7261 ttaacccgga gaatcttccc ggtatatcca cctgagtaca tttcatcctc cttttatttg
     7321 gtcttactca aattttaaaa actctgccct gtgctgcata gtaccgctgt tttctgcttc
     7381 tcccaccatg atttaatgga gtgagagacc tccatcgtgt acgatcatct ggcctgtaat
     7441 aaaggcgcta tcgttagttg acaaaaagag ggcaagacct accaggtctt caggcatact
     7501 tggtcttttt atacaccgac cctgtagttg taattcatca aggggaggca gaggtaaagc
     7561 tttgccgcga tcaaactgat cccccccttc cgagtgtgtg aagccaggag cgattgtatt
     7621 aacgtttatc atgaaatcgc taagctcacg tgccatgcag cgggtcaacg ccataacagc
     7681 tccttttgat gctacgtagt gtggcattcc tggtatgccc tccaagatgg atgccgaaga
     7741 gatattcacg atcttgccgc ctttttcctt catgtaaggg aatacggctt taatacagtg
     7801 gaaaacccca cttgtgttga tttctattac tttcttgaat tcttcgctgc tcaactcggt
     7861 gaaaggtttg agcactatat tggcaaatac agcagcattg ttaatgagaa tatcaatgct
     7921 gccaaatttg tcatacgcag ccttagccat agccatgcat tccggctcct tgctaacatc
     7981 agttttcacg aagagcgctt cacttcctgc atcagctact tttttcacag ttttagcccc
     8041 atcggaaata tcagcaatga cgattttggc tccttctttt gcaaaacctg cagcgaatgc
     8101 agcgccaatt ccctgagaag cacctgttat aatcgctatt ttgtccttaa gtctcatgta
     8161 gtaccctcct tttcaaataa aaaacctcct ttgccctgca tcgggcaaag gaggcatcct
     8221 tacctccagc tcactgaata cgttttttta tcggcaatct tattcaaatt atatgtgcac
     8281 catgaaagcc atgttaaaaa cattgtatcc agtcaatact tatcttttgc cccattctgg
     8341 agccagcttg agctggtatc cgccttttac agggggataa aagagcatct gcttcccgtc
     8401 ccaccactga ttcgctgtgc tggggaacaa ggctaatccg atctcgtcaa atttcaggtc
     8461 ccccatgacc gtccctttgt actcagcatt tcgcaaggca tctctgacag cggtcttatc
     8521 gtaggatttt gctctttcta tcgccataaa aaggacctgc gcagtaccgt aaaaggcacc
     8581 tatgctgaca ctgcgtttct taaacttatc ggtatatcgt ttcccgagtt ctttgcataa
     8641 agggtatggg aactcttccg accagaaacc atcgcatata acatagttgg catctttccc
     8701 cagagcttgt gcaaactcaa ctgcccatgt ccccttccat ccgtgcatat acttgacatt
     8761 aagctttgcc tcttttatct gacggaccaa tgtaatggca tctgtagggg ctccataaag
     8821 cagtatggcg tccacgtttt tcgctttcat cttgagtatc atagaggagt aatcctttgc
     8881 tccgatggcc cagggttcat caacagcaaa ggtgacaccg tgtgtttttg catgagatct
     8941 gaagcctccc ccaaatgcct gaccgtcagg actatcctcc atgagaaggg ccacattttt
     9001 cggcctgtcc tccttcttca gcagagtttc ccagattatg aaggggacct cagatgctcc
     9061 aacggctgag aaaaacatta cactggccca cttggccttt tgatcacgaa acatttccgg
     9121 ccaatgagtt gtgcagtgat ataccatctg atatttctcc gccgtattga tggcaggaac
     9181 actcatggtt gcagaatggg tgccaagcag gagatcaacc ttgtccacct tgataagctt
     9241 ctctatggct cctgccactt ttgccggttc gctttcatcg tcagcgaaga ccaatcttat
     9301 aggcaatttc ttattggccg attttatgaa gatacctccc ttcttgttcc agtctgcaac
     9361 cgcctgctca taggcccatt tcagttccag accgtccggt gccagagggc ctgtcatatg
     9421 aacaggagca ccgatgagga tttccttacc ggcagccatt gcgaaatttg aaaataccaa
     9481 acaaatgatg ggaatggaac aaaacaagcc gaaccaccaa aatattcttt tacttttcat
     9541 ttcatgccct ccttttttat ttaatcctat tcgttattcc tctcctcaaa gaggttaacg
     9601 ggaatacgca acctcacctc ctttcgatta ccgggacttg tgtctacctg tgctgaagtt
     9661 tcggaagaca ggtagtccct caccctgcaa agccgtgtgg aacttcccct tcacgctcat
     9721 tacaatcgca gtataacatg caacacccgt gtaaactccc acggatactt gttgacgcaa
     9781 acacgtgagg aaaacacttt caatcgtgcg tataaccatt ctacacccct ttcttccata
     9841 gtctaataat cccattgggc atgaaaagca tgacaagaat tataataatg ccggagaata
     9901 tggggtggat accaggcaag tagatgctgg tgacagtaaa aacagcagtc atcaggaggc
     9961 tgcccaggat aggtccgacc actgtgccgc ttccacctat gacgcacata agtaccggat
    10021 aaagagtcca tctgagaata agaaaaccct caggacttac ctgaaacaga tagtaggcat
    10081 aaaggcttcc gcacaggccg gaaagaaagg cacttattgc aaatgcaagc accttgtatt
    10141 tgagtatata gatgccactc gaggaggctg cagtctcgtc ctcgctgatg gccacaagtg
    10201 caagcccgac tcgtgacttt gtcataaaat agaccacacc agtggcgagg aaggcaagaa
    10261 agagagcggt ccagtagtga ggtcgcattg tggtgtagac acttgatgga agcataatac
    10321 cgatgggtcc cccggtaaat tgtccccctt tgatacatac cacctttaga atctcgccga
    10381 atccgagggt accgagggcg aagtaatcac catggagttt ggaaagcagc ggtatgccga
    10441 ttgcgagagc aaatatcaca ggtaccaatc cgcttaagat catagtaaca gggtcgaagt
    10501 agtagttctc ccagctgaat aaatctgcac gccagatcat tgcagtggta taggcaccaa
    10561 gggcaaaaaa ggcgtgcgtt ccgagactga cctgtccagt gtaaccagca acgaggttcc
    10621 agccctgggt caacacgata tgacaaaagg tgagaaaaag gaaatagacc aggtacgatt
    10681 tttcagaatc agagacaaa
//
LOCUS       DAOOMC010000098        10576 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdus sp. isolate AMR_MDS_2685
            NODE_7115_length_10576_cov_5.158730, whole genome shotgun sequence.
ACCESSION   DAOOMC010000098 DAOOMC010000000
VERSION     DAOOMC010000098.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864760
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdus sp. (wastewater metagenome)
  ORGANISM  Syntrophorhabdus sp.
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae; Syntrophorhabdus.
REFERENCE   1  (bases 1 to 10576)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10576)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,729
            Genes (coding)                    :: 2,708
            CDSs (with protein)               :: 2,708
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 7 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10576
                     /organism="Syntrophorhabdus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7115_length_10576_cov_5.158730"
                     /isolate="AMR_MDS_2685"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2699756"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..443)
                     /locus_tag="PK125_10085"
     CDS             complement(<1..443)
                     /locus_tag="PK125_10085"
                     /inference="COORDINATES: protein motif:HMM:NF013974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate dehydrogenase subunit
                     beta"
                     /protein_id="HPB38489.1"
                     /translation="MKKLMSGNAAAGEAAVLAGCTCYFGYPITPQNELTEYMAKRVPE
                     AGGVFIQSESEIAAINMVLGASVAGARAMTSSSGPGISLKQEGISYLAADELPAVILN
                     MVRGGPGMGNISPAQSDYLQATRGGGHGDYRTIVLAPSSVQEIAD"
     gene            complement(440..673)
                     /locus_tag="PK125_10090"
     CDS             complement(440..673)
                     /locus_tag="PK125_10090"
                     /inference="COORDINATES: protein motif:HMM:NF024247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HPB38490.1"
                     /translation="MVTQATKGYIEIDQERCKGCSFCIEYCPKKAIFLTDKLNLKGYF
                     VATYKEGNGCTGCAICAIVCPEVAIEVYRGGTA"
     gene            819..1292
                     /locus_tag="PK125_10095"
     CDS             819..1292
                     /locus_tag="PK125_10095"
                     /inference="COORDINATES: protein motif:HMM:NF022214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2569 family protein"
                     /protein_id="HPB38491.1"
                     /translation="MNGQQSELKGVKGWLLLLCINLSILDPFANLFNLAIGTHLGRQY
                     FEQNPALQKLMVINGVCSIALAVYSIYAGISLWRVLSNAVITAKKYLGAAFAYSILSL
                     FLPHLIGLSEEIQKEVGATSLFNSFVTALYLYAWYQYLQRSKRVKATYIAIGSEQ"
     gene            1653..2195
                     /locus_tag="PK125_10100"
     CDS             1653..2195
                     /locus_tag="PK125_10100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB38492.1"
                     /translation="MNKIIVDIDNTLWNLAPVFFEYLRRYNPEIPIEELKRGETGLKR
                     YIPREDLYGILKEIHMRQDQFEPYPEAQQFLAALKEMGLYVIIASIRDEEARDATERW
                     LKKYELMYDELHLSNDKTVLFNDVWAIVDDSIWTLDKAAHAGIVRTGLRNVWNDGRGH
                     PLFDTLPQILDYLKRRYSSK"
     gene            2342..4321
                     /locus_tag="PK125_10105"
     CDS             2342..4321
                     /locus_tag="PK125_10105"
                     /inference="COORDINATES: protein motif:HMM:NF024850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HPB38493.1"
                     /translation="MQLIAKRQDGNELLVGGIFSLTGYLSWSGDYKKKGAELKIEMIN
                     EAGGINGNRLKLVAYNDRSSPELAAKIAEMLVLKHRVIAMVGTGSLPISGAVARVANK
                     YKVPTFLNSGYTIDPLSDLFVFNTSHKTEFAVAGSFGHFVERGINRIALLMPRGPLGD
                     LGSWLGRQVGRELGIKIVGEERFDVSVPEMTSQLERLKSLKPLALFSFVTGEPAARLV
                     TKMAQLGMNISLLVSHGNANPAFLKLVSRAPIPIIVPSGRTMILDSIPEEDPCRNKMT
                     DFGERHTRRFGEPANYCSAESADAIDLLAEGLRSSGRPDGRILRDAVEAIKQFEGMQG
                     VYDLSPIDHYGTQVEHMVLLDVQDGIWHLTKTLSSVGVFEAIHSDNKKKLVFRLARAF
                     NEPSTEALKLAYETPGSTRRLAAQVGLSCTNLKGDPCFAVKLSSREKQELIQAIREED
                     YTKAKESLCRSLTISLLQYYEAFEPLKLAVSELFHALFDAAASEEGVDLEKLIELKSK
                     YVLEWTDLKGQEALCFWTVKAFREVIELISERQRERGTGLLKNILRFIEIHFAENLTV
                     DRIAREVCLSPSRLIHRMKSQYGLTLSDCIAKTRMDKAKALLKDTDMTICEIAHEVGY
                     VDQGYFTRVFKKCLNKTPGNYRESSRLGFPSNRDS"
     gene            4515..5669
                     /locus_tag="PK125_10110"
     CDS             4515..5669
                     /locus_tag="PK125_10110"
                     /inference="COORDINATES: protein motif:HMM:NF024850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HPB38494.1"
                     /translation="MKKFFVLIILCSVFVIFTGVGPIMAQTKAPYKIGGIFPLTGYLS
                     WLGEYKKKGSELKVELINKAGGINGRQLELVVYDDQSSPETGSRVAQRLVSKDGVIAM
                     IGTASVPISGAVASIGNRYKIPTIIGSGYEVDSKKDHFVFNSAHKTDFAVERPFEYFK
                     GKGITKVALLMPIGPLGELGGGLARKYAPKYGITIVGEERFDVKAPDITAQLAKLRTL
                     GPQAVFSFCTGEPAALVARSMAQLGMNIPVLVSHGNANPGFLKLVSNVNIPILVPAGR
                     AAAPESIPDNDPCKRVILDFNKAHQQKFGEPANYYSAEMVDAVDIIAAALKVVPTADG
                     EKLQDAIEKTKGLVGMNGIYNFSTTDHHGTVLQDMMVLTVKDGKWQVLLK"
     gene            5734..6600
                     /locus_tag="PK125_10115"
     CDS             5734..6600
                     /locus_tag="PK125_10115"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HPB38495.1"
                     /translation="MTDFSHIVQFFFSGLTSGSMYALIAVSLVIVYKVSLLICFAQGE
                     FFVIGALAMVTLVSKGLPMPLAFVLSILISTVLGALVQRMLIKPIQNAGIGNLIVMTI
                     AISLTLRGAALLVWGKESHILKPFSSGAPIPVLGATLQPQVLWIISITIVVLIMIWIF
                     FEKTKLGIAMRACAENRLGASLMGISTDKAALLAWAWGSGIGALAGMAVAPLLFMQYG
                     SGVMPMVKGFIAVSIGGLSSIVGSVVAGLLLGTVEAYTIGLISSKFSDTIVFSLLMVV
                     LIFKPSGLFGKG"
     gene            6610..7566
                     /locus_tag="PK125_10120"
     CDS             6610..7566
                     /locus_tag="PK125_10120"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HPB38496.1"
                     /translation="MKLLKQFWWILPLAFIAFYPVTLAGGSPYWSGLLIFVGIYVILT
                     VGLDLLMGYTGQISVGHAAYFAIGAYASGILTAKYGFSPLGAMVIGMVLTGIVAWALG
                     RPVLALKEYYLAMATLAFNEIVVTLIIGLEWLTGGASGLRDIPSFSIFGLVLSNHVHY
                     YYFVWAIVIIVIACSLAVVRSPFGRTLVAIHSDEVAARAFGVDTAKYKTRIFVLASVF
                     ASMAGSLFTHYMGFLAPDDFGVLTSINILVMLFLGGIGTIFGPILGAFFMKLLPELTY
                     QIHDYELLLSGLILIIVLVFMPKGLFGIFTAIWEKMFAKADK"
     gene            7563..8366
                     /locus_tag="PK125_10125"
     CDS             7563..8366
                     /locus_tag="PK125_10125"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPB38497.1"
                     /translation="MTDKRDAILSVKNVTKTFEGIRALEDVSFDVKSGHIKALIGPNG
                     AGKTTLLNAINGIMSPDSGHIHFLGHDVVGHSADRVAGLGLSRTFQLIRMFTINNATV
                     LDNVLLGAHMQLKPTLFESIFSRGKVNKAERKACDRAMELLRFVGLEKAADAAPGSLP
                     FGNQRLVELARSLMTNPKMLLLDEPASGLNDTEVESFTELLRSIREKGITILLVEHNM
                     KLVMNIADDIVVLDFGKKLAEGTPQAICANPQVIEAYLGTTQTECRLEP"
     gene            8363..9073
                     /locus_tag="PK125_10130"
     CDS             8363..9073
                     /locus_tag="PK125_10130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013801934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPB38498.1"
                     /translation="MMLKAEAISASYGRVKVLKDIDMSVESGEIVCLVGANGAGKSTL
                     LKVISGIVPAGSGKFLFEGQDITNKKPDFIVKAGLSHVPEGRQIFADLTVRQNLILGS
                     YVHNLPKQEMAKLFDSVFELFPVLKSRLMQKAGTMSGGEQQMLAIGRGLMSQPKLLLL
                     DEPSLGLAPLVVETILKVIQNLRSTGISILLVEQNVNAALQISDRAYVIETGAIVTEG
                     KARELMENDEIKKSYLGM"
     gene            9130..9810
                     /locus_tag="PK125_10135"
     CDS             9130..9810
                     /locus_tag="PK125_10135"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013756.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HPB38499.1"
                     /translation="MHYDPDKNDHGLAHSPFKSCVVPRPIGWISTVSPEGIHNLAPYS
                     QFQNLTFDPPYVMFAANQNTLGKRKDSVVNAEQTGEFVYNMATYELRDAMNRSAPEVP
                     PDIDEFELAGLTKAASIRVKPCRVAESPIQFECRYHQTIRLPGNGLMGTVDIVIGRVV
                     MIHIKDDVIRPDGRLDILKIRPLARLGYYDYTTVDSIFEMVIPGNNEALLTGLEGAAG
                     GSSTKSAQ"
     gene            complement(10036..10392)
                     /locus_tag="PK125_10140"
     CDS             complement(10036..10392)
                     /locus_tag="PK125_10140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB38500.1"
                     /translation="MCQTTSVPRTSWPDILVAKIHNIREPLCAGIDHADKGNSLSVYA
                     YAVRGAFFDGYGILDHLSKADSLKERNDKSKASIGSHLSFGEGNFDFVYLRHCGRISV
                     HSFVPPLVGVDCLVDN"
     gene            10377..>10576
                     /locus_tag="PK125_10145"
     CDS             10377..>10576
                     /locus_tag="PK125_10145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB38501.1"
                     /translation="MLFDTTTTLTRMFNKLKSLKTVDVLSCNVWAYLAQLIPWNTIRE
                     DWLTFDSTVLLRYGEQEGANTSS"
BASE COUNT         2823 a         2307 c         2548 g         2898 t
ORIGIN      
        1 aaatctgcta tttcctgtac cgatgaagga gcgagcacaa tggtgcgata atcaccatgg
       61 cctccaccgc gggttgcctg taaataatcg gactgagcag gagagatgtt tcccatgccc
      121 ggacctccgc gcaccatgtt gaggataact gcaggaagtt catctgcggc aagatatgat
      181 atgccttctt gtttgaggct gattccgggg ccacttgagg atgtcatggc ccgtgcacct
      241 gccacgcttg ctccgagaac catattgatg gctgcaattt cactctctga ctgaatgaaa
      301 acaccaccag cctccgggac tctttttgcc atatattccg taagctcgtt ctggggcgtt
      361 atggggtagc caaaatagca tgtgcatcca gccaggactg cagcttctcc ggcagctgca
      421 tttccactca tcagtttctt catgcagttc caccccgata gacttcaata gctacctcag
      481 ggcacacaat ggcgcagatt gcacaaccgg tacatccatt cccttccttg tatgttgcaa
      541 cgaaatatcc cttgaggtta agtttgtccg ttaggaaaat agctttcttc gggcagtact
      601 ctatgcagaa ggagcaccct ttgcagcgtt cctgatctat ttctatatat cctttggtgg
      661 cttgtgtaac catcttactg taataaaacg ccttatcgta gcgtaatcac atgcttcttg
      721 tcaaatattt tgtccgtgcg tattttcata cccaaggcaa ttcagatgtg gtaaaaatga
      781 aactatgtta tcaaaaaagg ttgggaggat aaagggagtt gaatggacaa caatctgagt
      841 tgaaaggggt gaaggggtgg ctgttactcc tttgtatcaa tcttagcatc cttgacccat
      901 ttgctaatct ttttaatctt gccataggaa cacacctcgg aaggcagtac tttgagcaaa
      961 accccgcatt gcaaaaacta atggttatta atggagtatg cagtatagcc cttgcagtct
     1021 atagtatcta tgcaggtata tctctctgga gagttctctc taacgctgta atcactgcca
     1081 aaaaatacct tggggcagct tttgcctact caattttgtc cctttttctt ccgcacctga
     1141 ttggcctatc cgaagagata cagaaagaag taggtgcaac gagtcttttc aacagctttg
     1201 tgactgcttt gtacctctat gcctggtacc aatacttaca gcgatcaaag cgagttaagg
     1261 ccacgtatat tgcaatagga tcagagcaat agttagtatc tctggatgac attctatcca
     1321 aatatgactt gccttgtcca tgtagcagtg atccaagttc ctgagtccta ataatttcag
     1381 atcgatcagg gctaccgaaa taaaatagat ctgagcctgc tttatagtca tgtttcaatg
     1441 tccaatctgc gctcactaca gaattaatag ctatacaaca gtaggaaatg atactttgga
     1501 tttaagatca aaatacattt cgtaaacaat gcgagccgac tcaaaggttg caggttgttt
     1561 gaacctgggt tgttatcatt ctttcgattg tacgttccca aagcatattc tattgacaac
     1621 agagaagcac actggtacca taggcgctgg ttatgaacaa aatcattgtt gatatagaca
     1681 ataccctctg gaatctggca ccagtattct ttgaatacct cagaagatac aatcccgaaa
     1741 taccaatcga agagttgaaa cgtggtgaga ccgggctaaa aagatacatt cccagagagg
     1801 atctgtatgg gatcttgaaa gaaatccaca tgagacagga tcaattcgag ccatatcccg
     1861 aagcacagca atttcttgcg gctctgaagg agatgggatt atatgttatc atcgcaagca
     1921 tcagagatga ggaggcacgg gatgcaacag agaggtggct caaaaagtat gagctaatgt
     1981 atgatgagtt gcatttaagt aatgataaga ccgttttatt caatgatgtc tgggcaatcg
     2041 ttgacgatag catatggacc ctggataaag ctgcgcatgc aggaatagtc cggaccggac
     2101 tcaggaacgt ttggaatgat ggccgcggac accctctttt tgatactctc ccgcagatac
     2161 ttgattattt aaaaaggcga tattcaagca aataagcatt tgtttaaacc tcactggctt
     2221 actgtataat cattggccat gtttctcata tgtctgaaat caaaatacca tttgacttct
     2281 atatggaaca ttgtagaata gtacaatata ttgcaaggct ctgggggttt atctcaagga
     2341 tatgcaactc atagcaaaaa gacaggatgg taatgaactt cttgtcgggg ggattttttc
     2401 cttgacaggg tacctgtcct ggagtggaga ttacaagaaa aaaggcgcag agctgaaaat
     2461 tgagatgatc aatgaggctg gtggcattaa tggaaatcgc cttaaacttg tcgcctataa
     2521 tgaccggtct tcgcctgaac tggcagcaaa aatcgctgaa atgctggttc tcaaacacag
     2581 agttattgcc atggtcggca caggctctct gccgatctca ggcgctgttg cccgtgttgc
     2641 aaacaagtat aaagtcccaa cgtttctcaa ttccggttat acaatagatc cgctgagcga
     2701 tctgttcgtt ttcaatacct cccataaaac cgaatttgca gtcgccggct cttttgggca
     2761 ttttgtcgaa agagggatta acagaattgc tcttctgatg ccaagaggtc cgttaggtga
     2821 cctgggtagc tggctgggtc ggcaggtggg cagggaatta ggcattaaaa tagtcggaga
     2881 ggaaaggttc gacgtcagcg taccggaaat gacatctcaa ttggagcgat tgaaaagcct
     2941 gaagcctctg gcgcttttct cctttgtgac cggggaaccc gccgctcgcc tggtaaccaa
     3001 aatggctcag cttggaatga atatttcctt gctcgtatct catggtaacg ccaaccctgc
     3061 atttctgaaa ttggtttccc gtgctcctat acccataatc gtgccctcag ggaggaccat
     3121 gattcttgat tccatacctg aggaggatcc atgcagaaat aaaatgacag acttcggcga
     3181 aaggcatacc cggcggtttg gtgagccagc caactactgt tcagcggagt ccgccgatgc
     3241 cattgacctg ctcgctgaag gtctgcgatc ttcaggccgg cctgatggaa ggattctgcg
     3301 ggatgcagtg gaagctataa agcagtttga aggcatgcag ggtgtatatg atctgtcacc
     3361 catagaccat tatggcaccc aggtcgaaca tatggtattg ctggacgtcc aagacgggat
     3421 atggcatctg acgaaaacgc tttcatcggt tggagtgttt gaagcgatcc atagcgataa
     3481 caaaaagaag ttggttttca ggttggctcg tgcattcaac gaaccatcca ctgaagcgct
     3541 taagcttgcc tatgagacgc cggggtcgac aagaaggctg gctgcccagg tcgggttaag
     3601 ttgcacaaac cttaaaggcg atccttgttt tgctgtcaag ctttcaagtc gggaaaaaca
     3661 ggagttgatt caggctatcc gtgaagaaga ttacaccaag gcaaaagaaa gtctttgccg
     3721 ttctctgacc atttctcttc tccaatatta tgaagccttt gaaccgctaa aacttgccgt
     3781 gtcagaactg tttcatgccc tgtttgatgc agcggccagc gaggaggggg ttgaccttga
     3841 aaaactcata gagcttaaat ctaaatatgt actggaatgg acagacctga aaggtcaaga
     3901 agcgctttgt ttttggacag tgaaagcgtt cagggaagtt attgaactaa tcagcgagag
     3961 acagcgggaa agaggaacag gattattgaa gaatatcctc agattcatag aaatccattt
     4021 tgctgagaat cttaccgttg acaggatagc ccgtgaggtc tgtctcagtc cttcacgcct
     4081 tattcatcgg atgaaaagcc agtacggcct gacgctttct gattgtatcg caaaaacccg
     4141 gatggataaa gcaaaagcat tattgaaaga cacggatatg accatctgtg aaatagccca
     4201 tgaagtggga tatgtggatc aaggctattt tacgagggtc ttcaagaaat gcctgaataa
     4261 aacccccggc aattacagag aaagttcccg gttaggtttt ccatcaaacc gcgactctta
     4321 acagccactc gaatttgttc gacactttta tccaaaaaaa gaactgaata atacaagaca
     4381 caagtttcca cagatgctta aattagacta aagaagtttt ctttttgttt gataatgttg
     4441 aaatcaagca tttatgcaat agtattcaca ttgcaacagg gcttgaaata aaaaaacaat
     4501 ggggaggcct tcttatgaaa aaattttttg ttttgataat actttgctct gtttttgtga
     4561 tattcacggg agtggggcct attatggctc agacgaaagc gccctataag atcggcggaa
     4621 tattccctct aacaggttac ctgtcatggc tgggtgagta caagaaaaaa ggatctgaac
     4681 tgaaggtaga gttgattaac aaagccggtg ggattaatgg cagacagctg gagcttgttg
     4741 tttatgacga tcagtcctct cctgagacag gctccagagt cgcccagaga ctggtttcca
     4801 aggatggagt catagccatg atagggactg caagcgttcc catctccggc gctgttgcat
     4861 ctattggaaa cagatataaa attcctacaa ttattggttc cggttatgag gttgattcca
     4921 aaaaagacca ttttgtcttt aacagcgccc acaaaacaga ttttgccgtg gagcgtcctt
     4981 ttgaatattt taagggtaaa ggcataacaa aggttgctct tttgatgccc atagggcccc
     5041 ttggcgaatt aggcggtggc ctagcacgga agtacgctcc aaagtatggg ataacaatag
     5101 tcggtgaaga gcgctttgac gtaaaggctc cagatattac agcgcaactt gcaaaattgc
     5161 gcaccctcgg tccgcaggca gtcttttctt tctgtaccgg tgagcctgct gctcttgtag
     5221 cgagaagcat ggcacagctc ggtatgaata tacctgttct cgtatcgcac ggtaatgcca
     5281 atcccggctt cctgaaactt gtttccaatg taaatattcc aattctcgtt ccggcaggaa
     5341 gggctgcagc gccggagtcg attcctgaca acgacccatg caagagagtt attcttgact
     5401 tcaataaggc gcaccagcag aagttcggtg agcctgccaa ctattattca gcagaaatgg
     5461 ttgatgcagt ggatatcatt gcggctgcgc taaaggttgt cccgactgcg gacggtgaaa
     5521 aacttcagga tgccattgaa aagacaaaag ggctggttgg tatgaatggt atttataatt
     5581 tctccacaac agaccaccat ggaacggtcc ttcaggacat gatggtcctt acggtaaagg
     5641 atggaaaatg gcaggttctg ctgaagtaac aatgaagggt ttattgcaag gccactgagg
     5701 atatacattt gatattcttg aggattagga cagatgactg atttttctca tattgtacag
     5761 tttttcttta gcggccttac gtcaggaagc atgtatgccc tgatagcagt gagccttgtg
     5821 attgtctata aggtttccct gctcatttgt tttgcccagg gtgagttctt tgtaatagga
     5881 gcccttgcaa tggttaccct tgtctcgaaa gggcttccga tgcccctggc atttgtcctg
     5941 tcaatcctga tttcgacggt gcttggcgcc ctcgtgcaac gcatgcttat taagccgatt
     6001 caaaatgcag gcatcggtaa ccttattgta atgactatcg ccatttcact tacgctcaga
     6061 ggggctgcac ttctcgtctg gggaaaggag tcgcatatcc tgaagccttt ttcttccggc
     6121 gcgcctattc cggtcttagg cgcaacactt cagccccagg tcctttggat cataagcatt
     6181 acaatagttg ttttaatcat gatctggata ttttttgaaa aaacaaaact gggtattgcc
     6241 atgagggctt gtgcggaaaa ccgccttggg gcaagcttga tggggataag cacggacaag
     6301 gcagctttgt tagcctgggc atggggatcc ggcattggtg cgctggcagg gatggctgtt
     6361 gcacctctcc tgtttatgca gtacgggagt ggagtgatgc ccatggtcaa gggatttatc
     6421 gcagtgtcta taggtggttt gagcagcata gtgggctctg ttgtagctgg attgctcctt
     6481 gggactgttg aggcctatac aatcggtctc atctcttcaa agttttcaga tactattgtg
     6541 ttttccctgc tcatggtggt tctcatcttt aagccaagcg ggcttttcgg caaaggatag
     6601 gagatccatg tgaaactgtt gaaacaattc tggtggattc tccctttagc attcatagcg
     6661 ttctacccgg tgaccctggc gggtggatct ccttactgga gcggacttct tatttttgta
     6721 ggtatctatg taattctgac agtgggtctt gacctgctta tgggttacac aggtcagata
     6781 tcggttggtc atgctgccta ttttgccatt ggcgcttatg cttccggcat cctgactgcc
     6841 aagtatggtt tttctcctct cggagctatg gttatcggta tggttctgac aggtattgtt
     6901 gcctgggctc ttgggaggcc tgtccttgcc ctgaaagaat attatcttgc aatggcaacc
     6961 cttgccttca atgagattgt cgtgaccttg attataggcc ttgaatggct caccggaggg
     7021 gcatcgggtc tgcgtgatat tccatcgttc agcatatttg gtcttgtcct gagcaaccat
     7081 gtgcactact actattttgt atgggcaatt gtaattattg ttattgcgtg cagtctggca
     7141 gtggtgcgct caccttttgg aagaacattg gtcgctattc acagtgatga agttgctgcc
     7201 cgtgcattcg gtgttgatac agcaaaatat aaaacaagga tctttgttct tgccagcgtc
     7261 tttgcgtcca tggctggatc tttgtttacc cattatatgg gatttctggc ccctgatgat
     7321 ttcggggttc tcacgtcgat caatatcctc gtaatgcttt tccttggagg aatcggtaca
     7381 atattcggcc cgatccttgg tgctttcttt atgaagctcc ttccggaact tacctatcag
     7441 attcacgact atgagttatt gttgagcgga ctaattctta tcattgttct tgtttttatg
     7501 cccaagggac tttttggcat ctttactgcc atatgggaaa agatgtttgc aaaggcagat
     7561 aaatgacaga caaacgcgat gccattctga gtgttaagaa cgttaccaag acctttgagg
     7621 ggattcgtgc ccttgaagat gttagttttg atgtgaaatc aggccatata aaggctttga
     7681 taggtccgaa cggcgctgga aagacaacgc tgttgaatgc aataaacggg ataatgtctc
     7741 cagatagcgg acatattcac tttcttggcc atgatgttgt tggtcacagc gctgacagag
     7801 ttgccggcct cggtctttca aggacctttc agcttatccg gatgtttaca atcaacaacg
     7861 ccactgttct cgacaatgta cttcttggtg cccatatgca gttgaagccg actctttttg
     7921 aatctatttt cagtagagga aaagtaaata aagcagaaag aaaagcctgc gacagggcaa
     7981 tggaactttt gagatttgtt ggtcttgaga aagcggcaga tgccgctccc ggctctctgc
     8041 cattcggaaa ccagcgactc gtagagttgg ctcgttcgtt aatgacgaat ccaaagatgc
     8101 tcttgctgga tgaaccggca tcaggtctta atgacaccga ggtggaaagc ttcacggagc
     8161 ttctccgatc gattcgtgaa aaaggcatta caattctttt ggtggaacac aacatgaagc
     8221 ttgtaatgaa cattgccgat gatattgtcg ttctggattt tgggaagaaa ctggcagaag
     8281 gaactcctca agccatctgt gccaaccctc aggtaataga agcgtatctg gggacaacac
     8341 aaacagaatg caggcttgaa ccatgatgtt aaaagcggaa gccatttctg caagctacgg
     8401 cagagttaag gtactgaaag atatcgatat gtcggttgaa tcaggcgaga ttgtttgtct
     8461 tgttggggca aacggcgcag ggaaatcgac tcttttaaaa gtaatatcag gaattgtacc
     8521 ggcgggcagc ggaaaatttc tgtttgaagg ccaagatatc acaaacaaaa agcctgattt
     8581 tatagtaaaa gcaggtctca gccatgtgcc tgaaggccgt cagatctttg cagatcttac
     8641 tgttcggcaa aacctcattc taggatccta tgttcacaat ttacccaaac aggaaatggc
     8701 gaagctcttc gattctgttt tcgagctttt tcctgtacta aaatcgcgat tgatgcaaaa
     8761 agcgggcacc atgagtggtg gcgagcagca gatgctggca ataggtagag gtctaatgtc
     8821 tcaacctaag cttcttctcc ttgatgaacc atcccttggt ttagcgccgc ttgtggtgga
     8881 gaccatcctc aaggtcatac agaaccttcg ctccacagga atctctatct tgcttgtgga
     8941 acagaatgta aatgcagcgc ttcaaatatc ggaccgggca tatgtgatag aaacaggcgc
     9001 tatcgtgact gaagggaaag cccgggaatt gatggaaaat gatgagataa aaaagagtta
     9061 cctgggtatg tagaccttct gcatagaacg cagcagcccg gtgagtctct tgatagggga
     9121 ggttacacaa tgcactacga tcccgataag aatgaccatg gcctggcaca cagtccgttt
     9181 aaatcatgcg tggttcccag gccaattggc tggatatcaa cggtcagtcc ggaggggatt
     9241 cataacctgg cgccatacag ccagtttcag aatctgacct ttgaccctcc ctacgtaatg
     9301 tttgcagcga atcagaacac cctgggaaag agaaaggatt ctgttgttaa cgcagagcaa
     9361 acgggtgaat ttgtttacaa catggctact tatgagttgc gggacgctat gaacagatca
     9421 gcgcctgagg tgccacctga catcgatgaa tttgagcttg cggggctaac caaggcggct
     9481 tcaatcaggg taaaaccctg ccgtgtggca gaatctccga tacagtttga atgccggtat
     9541 catcagacca ttcgactgcc cggcaacgga ctcatgggga cggttgatat cgtgatcggc
     9601 cgggtagtca tgatacatat caaagatgat gtcatacgtc ctgatggacg gcttgacatt
     9661 ctaaaaatca ggcctttagc ccgtcttggg tattatgatt acacaacggt ggattccatc
     9721 ttcgaaatgg tgatacccgg taacaacgag gccctcctca ccggactgga aggcgcagcc
     9781 ggcggttctt ctaccaaaag cgcacagtaa tgacctctgt ttaaagacgc ttcttaccta
     9841 ttctagcgtc catccagaaa ccctcgattt tcaatgaagt cggcattagt cactcgagta
     9901 agtcaacttg attgctcgga tggtcggagc ttggagccat gatttgtctg cgacacggtc
     9961 attgggccag tttaacctga aaaatgcatt tgcctccaat aaacccttaa caaaaaacct
    10021 cctcttgcag taggatcaat tgtctaccaa acaatcgacc ccgacaagag gaggaacaaa
    10081 actatgcaca gaaattctac cacaatgcct caggtaaaca aagtcaaaat taccttcacc
    10141 aaaagaaaga tgactgccta tggaggcttt gctcttatcg tttctttcct tcagagaatc
    10201 ggctttgctc agatgatcga gaatgccata cccatcaaag aaagctcccc gaacagcata
    10261 ggcatatacg gaaagactat tgcctttgtc ggcatggtct atgccggcgc agagcggttc
    10321 tcttatgtta tgtatcttgg caacaaggat gtctggccag cttgttcggg gtacagatgt
    10381 tgtttgacac aaccacaacc cttacccgaa tgttcaataa acttaagagc ctcaagaccg
    10441 tagatgtgct ttcctgcaac gtgtgggcat atcttgcaca actcatcccc tggaacacga
    10501 ttcgagaaga ctggcttact ttcgactcca ccgtgctcct ccggtacgga gaacaggaag
    10561 gagcaaatac gtcatc
//
LOCUS       DAOOMC010000099        10525 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdus sp. isolate AMR_MDS_2685
            NODE_7163_length_10525_cov_6.382904, whole genome shotgun sequence.
ACCESSION   DAOOMC010000099 DAOOMC010000000
VERSION     DAOOMC010000099.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864760
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdus sp. (wastewater metagenome)
  ORGANISM  Syntrophorhabdus sp.
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae; Syntrophorhabdus.
REFERENCE   1  (bases 1 to 10525)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10525)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,729
            Genes (coding)                    :: 2,708
            CDSs (with protein)               :: 2,708
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 7 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10525
                     /organism="Syntrophorhabdus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7163_length_10525_cov_6.382904"
                     /isolate="AMR_MDS_2685"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2699756"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            343..1500
                     /locus_tag="PK125_10150"
     CDS             343..1500
                     /locus_tag="PK125_10150"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPB38502.1"
                     /translation="MKITKKRNGTSAGSTDAMYQTVFENTGTATIIGEDDTTIYLANT
                     QFQNLCGCSKEQLEGKMKWADFIVPEDLPMMLQYHRNRRVNPDAVPKHYELRFHDCNG
                     VIRDMYITVDLIPGTQKSVLSFMDVTKLKQAEREVRELNEELEKRVKERTAQLEATNK
                     EIESFSYSVSHDLRMPLISIQAFSRLLIEKYASRLDAKGKRFLSAIQKSSKQMDQLIN
                     GLLALSKLKKQELKATEIDMGNLARDVFEELKTVNPGRVVRLVVHHPPTGVGDSSMIR
                     QVVVNLLSNAMKFTRKREEAVIEIGGSYDGDETIYYIKDNGVGFNMAEADKLFGVFQR
                     LHSADEYEGTGIGLTIVQRIITRHGGRVWAEAKPDEGATFYFTLPGKCVLK"
     gene            1625..1828
                     /locus_tag="PK125_10155"
     CDS             1625..1828
                     /locus_tag="PK125_10155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB38503.1"
                     /translation="MRALLSAFEKDSEAHVDIIVERRNQTHNAMPVRHKSSGVQAQRE
                     KLRDKKKGLSFSYKREQIIQPAG"
     gene            2164..2862
                     /locus_tag="PK125_10160"
     CDS             2164..2862
                     /locus_tag="PK125_10160"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HPB38504.1"
                     /translation="MDRNSYIPDSPYLTDNLTDHPEELSLILSISKPKRYKAGGLVYV
                     QGEESKPYFYLIEQGKVKISLLSKGGSERIIIIQEKNTLFGYAATFDGHPYFHTATAV
                     EETILRIIHINDFLNLVDKHPKVGLLIVVAFARVTRMLVLQIEDFSFLDAEKRVAHMI
                     YKLAFEIGQKTPRGLLIAKKVTQEDIARLAGLSRVSVSLALNHLEDLDILRKKRNIIE
                     VFDIKKLKDFIGYL"
     gene            2895..3722
                     /locus_tag="PK125_10165"
     CDS             2895..3722
                     /locus_tag="PK125_10165"
                     /inference="COORDINATES: protein motif:HMM:NF016777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HPB38505.1"
                     /translation="MKIDFHTHIYPDHVARQTVFTVQQRAGIDAYSDGTLEGLKRSMT
                     AAGIDLSVIAAVATKPEQVPSIQRWLTSIRQPGIEAMAVMHPADPLSSEQMRALKGEG
                     FRGFKFHPDYQGFFVDDPRMYPLYEAIAAEGMFVLFHAGMDRGLPCPIHATPKRLATV
                     HKAIPELTIIAAHLGGEGAYEETAEHLLGQNIYLDTSFVLRRMPGKFRERFFKEHRAD
                     RLVFASDSPWTDQRKEIHFFLQIPFLSDSDKEKICFSNAAQLLGLDTKRKDVERACQ"
     gene            complement(3745..4482)
                     /locus_tag="PK125_10170"
     CDS             complement(3745..4482)
                     /locus_tag="PK125_10170"
                     /inference="COORDINATES: protein motif:HMM:NF039354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6125 family protein"
                     /protein_id="HPB38506.1"
                     /translation="MNKKNTLNRKERIVSQIIDGLRLTFLHYGLWFKEVEYQLGLQSA
                     MELEEQTWQTAFPIMMKRLGKLLGFEVDPKGVPKQLLEKSEKDLQEILTAVSINWLAA
                     DGVWFQSVEKTFDMFTAKRCTDICWSRFSPLEAFHIKSLIGLPENGGLDALEKALNHR
                     LYSRINKHIFEHPSGDTLIYRMCRCRVQEIRNRKGMDDYPCKSGGIVEYATFARTIDP
                     AIRVECISCPPDPHPKGWFCSWKFTLI"
     gene            4628..5008
                     /locus_tag="PK125_10175"
     CDS             4628..5008
                     /locus_tag="PK125_10175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB38507.1"
                     /translation="MGEWVYCPRAVCPFYFLGLVRRKILTYIFHYASQKCKGNPRTSP
                     ENLSRLFFLFGALSLLDPDNEPFCTRKVDRVMSNADKTSVRGVTEVVEMGNIINDPEF
                     SAKASEMVSALIRQIRPIESVLTK"
     gene            complement(5037..5426)
                     /locus_tag="PK125_10180"
     CDS             complement(5037..5426)
                     /locus_tag="PK125_10180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB38508.1"
                     /translation="MDDELLFLIGINHSSASQAVTHVTNKEEWQYILATARANGWKPL
                     GTILDYEFQYQLVASQCEALDFDKHTLLDQFITDKCGRWKGGYLTPEHQIVTDDDARG
                     LRIALQRASASIELILFLSHGAFRIAG"
     gene            complement(5693..6166)
                     /locus_tag="PK125_10185"
     CDS             complement(5693..6166)
                     /locus_tag="PK125_10185"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HPB38509.1"
                     /translation="MRKIHIIAAVICFCFVCSFTVYAQQAGKQDSKIEYFKTPRPMAL
                     PFSDAARVGNILYLSGEIGVDYTTMKVVPGGIKAETKQAFDNIKATLEKYGSSMDNVF
                     KCTVMIADMKEWPLMNEVYVTYFKKDRLPARSAIGANGLALGAKVEIECLATVDK"
     gene            6376..7260
                     /locus_tag="PK125_10190"
     CDS             6376..7260
                     /locus_tag="PK125_10190"
                     /inference="COORDINATES: protein motif:HMM:NF021287.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043248 - proteasome assembly [Evidence
                     IEA];
                     GO_process: GO:0051131 - chaperone-mediated protein
                     complex assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAC2 family protein"
                     /protein_id="HPB38510.1"
                     /translation="MKIGGFELHDPVPECNESYAIATLRPWIDVNSIGSLVLKELKSR
                     YDAVELGKLYKPGHFYDFTRYRPTIDLEEGIRDISIPNSTIHYARREQESDLLLLRLL
                     EPHAHAEFYIASVLKLLKRFKVKKYILLGSMYDMVPHTRPLLVSGYGMGTGAVEELKR
                     TAVLPITYRGPSTILNLIAKESAELGIDAIVFIVSLPQYVVIEEDFMGKVRLMELLNS
                     LYNIPVDKKDFEKALEQRTSIAERVERTPEVKMVLPQLENVYDMRIRTMKTKEGPQLT
                     PEIEEMLWKTMGKDIGKA"
     gene            7269..8969
                     /locus_tag="PK125_10195"
     CDS             7269..8969
                     /locus_tag="PK125_10195"
                     /inference="COORDINATES: protein motif:HMM:NF017745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF885 domain-containing protein"
                     /protein_id="HPB38511.1"
                     /translation="MTSVCNKESTELEAVVKKYWEWRLREFPEFSTWTGDNRYNHKFT
                     DLSLEAIERRQSDERHMLEQVKNIDPSGLSGQDVLSHALLLWDLSLAVSAQQFPPVML
                     LTQISGPQLMFPQLVSVTSFQTLTDYNNYCARLAAFPVYLEQAAGLLRHGIELGWVQP
                     NGPLSGVPGQINGQIVSDIEKSPLYTPFLRMPDTVTEKERHRMKSEANKLITDLVFPA
                     MNRFRDFITDIYMPAGDRSPGVSALPNGEAYYAHCVREYTTTDLQVSEIHNTGVREVE
                     RIRVLMHGVIRETGFKGSLQEFVDFLRTDPQFYFSKSEDLVVAYRDIAKRADAELPAL
                     FSELPRLPYGVRAFPDFEAPNQVTAMYYPGAADGSRAGYFMVNTYRPDIRPKYEMETL
                     TLHEAVPGHHLQIARAQELRHLPDFRRNGSYTAFIEGWALYAESLGRQMGFYSDPYAR
                     FGQLMYEMWRACRLVVDTGIHSLGWSRQRSIDYIRENTAKTEQEIAVEVDRYIVLPGQ
                     ALAYKVGELAIRELRASAEKMLGKSFDIRTFHNALLDNGPLPLSILKNQMEEWIKGQA
                     "
     gene            8973..10388
                     /locus_tag="PK125_10200"
     CDS             8973..10388
                     /locus_tag="PK125_10200"
                     /inference="COORDINATES: protein motif:HMM:NF019850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase domain-containing protein"
                     /protein_id="HPB38512.1"
                     /translation="MNSIYKSDYAITIEEKQCRNIHFTGIFGSGMSALAQYLCFHGFI
                     VSGSDRLLLSEDAVSLRQSLERVGCLIVNQDGSGISTDTDVVCVSTAIEESNPDISAA
                     HARNIPVIHRSDLLAAIISSKRTIAIAGTSGKSTVTSMIFEFLTACGKSPSLISGASL
                     RRLENEGMIGNAFRGSSDLLVVEADESDGTIVKYHTDVAVVLNVSKDHRSVEEIKEFF
                     STLISKAKWAVTNSDDSILASLPASERFGRNLLASWRPDQEELLPMSVKLFRNGIEYH
                     LPLPGLHNLENLRAALCVCERFGCEGSDLASAVQNFQGVARRFCITETKQNVYVIDDF
                     AHNPAKIRAAVTAARGISEHVVSVYQPHGFGPTRFLRDEYITTFRAIFKEGDFLYLLP
                     IYYAGGTAQKDICSEDIIAGLCDVSFKAEAVIDRDQLLARLANNVRPGDCVLVMGARD
                     PSLPALVKRIIEIFDNSNSVS"
BASE COUNT         2925 a         2275 c         2483 g         2842 t
ORIGIN      
        1 cggctctttg tccttccccg tcaacccctg gctgggagac aatgctgcca accatgcctg
       61 atgtactttt gattggtgtt tgtatgcttg tctggaaaaa taagatgtaa tgaattttga
      121 tgctgtacac gccgggcatc tcagacagac tatgagattt gtggaattta gggacgcccg
      181 gagaatccaa atatcgggca tcagggacgt tggtgaaatg gcaacattcg cttcatttct
      241 caggatacta attacatagt tcaagttgat ggtaatcgtc ctaatttgct atgtttactt
      301 ttatcgggta caatttcatc atatgacaaa aaggaacttt tattgaaaat aaccaaaaaa
      361 agaaatggta catcggcggg ttcaactgat gccatgtatc aaacagtttt tgaaaataca
      421 ggaactgcca ccataatcgg cgaggatgat acgacaatat atctggcaaa tacccagttt
      481 cagaacctgt gtggctgctc aaaggaacag cttgaaggga aaatgaaatg ggctgatttt
      541 attgtacctg aagatctccc catgatgctc cagtaccatc gaaataggag ggttaaccct
      601 gatgcagtac caaagcacta cgaactcagg tttcatgatt gcaacggtgt aatcagagat
      661 atgtatatca cggttgacct catccccggt acccaaaaaa gtgtcctttc tttcatggat
      721 gttacgaagc ttaagcaggc tgaacgggag gtaagagagt taaatgaaga gttggagaaa
      781 cgggttaagg aacggactgc ccagcttgaa gcaacgaata aagagatcga gtcattctcc
      841 tactctgtct cccacgatct gaggatgccc cttatctcta ttcaggcctt ttctcgtctt
      901 cttatagaaa agtatgcatc ccgcctcgat gcaaaaggaa agcgtttcct cagtgccata
      961 caaaaatcat caaagcagat ggatcaactg atcaatggcc tgcttgccct ttcgaagcta
     1021 aaaaaacagg agctcaaggc aaccgaaatt gacatgggga atcttgcgag agatgttttt
     1081 gaagaactca agacagtcaa tccgggtcgg gtggtaaggc ttgtggttca tcacccgccc
     1141 acaggggtag gtgattcatc aatgattcga caggttgtgg ttaaccttct ctcgaatgcc
     1201 atgaagttta caagaaaaag agaggaggca gttatcgaga ttggtggctc ttatgatggt
     1261 gatgaaacta tttactatat aaaggataat ggtgttggtt ttaacatggc agaggctgat
     1321 aaacttttcg gggttttcca aaggctccat agcgccgatg agtatgaggg aaccggcatc
     1381 ggtcttacca ttgttcaaag gataataacc cgtcatggtg gccgtgtttg ggccgaggca
     1441 aaacccgatg agggtgcgac cttttacttt acccttcctg gaaagtgtgt tttgaaatag
     1501 aataaaaaaa gcaccagcat cagaacaaac cactgttacc tgtactggcg ccgcaacttc
     1561 cgtatcccca agccgaagcc tcgagaccac cataaaatga gcagttgtca gcactgagta
     1621 ttatatgcgt gcgctgctgt ctgcctttga gaaggattcc gaagcccatg tcgatataat
     1681 tgtggagcga agaaatcaga cgcacaatgc gatgccagtt cggcataaat cgagtggggt
     1741 tcaggcacag agagaaaaac ttagggacaa gaaaaaagga ttgtcatttt cgtacaaaag
     1801 agagcagatt atccagccgg caggttgaca tgttgacaga agaagcaacc agggatagat
     1861 taaatgcgct atccctggta ttgacggcaa cagcatctca tgctctatcc tctcttgttc
     1921 cgtatttgac agctatgaag cacgcaatta ccatggccac gattgctccg ataattacca
     1981 tcatatcaac tcacctcctt tcttttaata tgagtgtcta tatctttatt agcagagcta
     2041 tccatgtatg tctgttaagt atagaactat gtttggtgtc aaactgtgtt aagcagttaa
     2101 cattgaaaag aaacggtttt cggtgtacaa tctacgagat atgtgaacag aaggggggtt
     2161 tctatggata gaaatagtta tattcctgat tctccatatc taacagataa tctcacagat
     2221 catccagagg aattatccct tatcctctcg atttcaaaac caaaaagata taaagccggc
     2281 ggccttgtct atgttcaggg ggaggagagt aagccctatt tttatcttat tgagcaaggc
     2341 aaagtaaaaa tcagtctttt aagcaagggc ggctcagaaa ggattatcat cattcaagag
     2401 aagaacaccc tttttggata tgcagctacc tttgatggtc acccatactt tcatactgca
     2461 actgctgtgg aagaaacaat tttgagaatc atacatatta atgactttct gaatctcgtc
     2521 gacaagcacc cgaaggttgg tcttctcatc gtggtggcgt ttgccagagt tacaaggatg
     2581 cttgtgctcc agattgaaga tttctctttt cttgatgcgg aaaaaagggt ggcccacatg
     2641 atatacaaac ttgcctttga aattggccaa aaaacaccaa gagggcttct catagcaaaa
     2701 aaggttaccc aggaagatat tgcaaggtta gcggggctgt caagagtatc tgtttccctt
     2761 gccttgaatc acctcgaaga tctcgatatc ctgagaaaga agagaaacat catagaggtt
     2821 ttcgatatta agaagctcaa ggattttata ggttatctct gagctccaga aaagcagagt
     2881 ataaaaataa tgcagtgaag attgatttcc atacccatat ctatcctgac catgtagctc
     2941 gtcaaacagt ttttacggtt caacagcggg caggaatcga tgcctattct gacggtaccc
     3001 tggaggggct caaaaggtct atgactgctg ccgggataga tctttccgtt atagctgcag
     3061 tagcaacaaa gccggaacag gttccatcca tccagagatg gttgacaagc atacgtcaac
     3121 cgggcattga ggcaatggct gtcatgcatc cagctgatcc tttaagctct gagcaaatga
     3181 gggctctgaa gggagaagga tttcggggct ttaaatttca cccggattac cagggttttt
     3241 ttgtagatga tccacggatg tatcctttgt acgaagcaat agcggctgaa ggcatgttcg
     3301 ttctcttcca tgcgggaatg gatcggggac ttccttgtcc catccatgca acaccaaagc
     3361 gcctggccac agttcacaag gccattccgg agctcaccat aattgctgcc catctgggcg
     3421 gggaaggggc ctatgaggaa acagcggaac atttacttgg acagaacatt tatttagaca
     3481 cttcttttgt actgcgcagg atgcccggaa agttcaggga gcgatttttt aaggaacatc
     3541 gggcagaccg tctggtgttt gcatcagata gtccgtggac ggatcagaga aaagagatcc
     3601 atttttttct gcaaattccg tttctatcgg acagtgacaa ggaaaaaatc tgtttttcta
     3661 atgctgcgca gctattggga ttggacacta aaagaaagga tgttgaacgg gcttgtcaat
     3721 aattctaagg aggagcctgc cctttcatat aagagtaaat ttccaggaac agaaccagcc
     3781 ctttggatgc ggatccggag gacacgagat gcattccacg cgtatggcag ggtctatagt
     3841 cctggcaaag gtagcgtatt cgacaatgcc ccctgatttg catggatagt cgtccatccc
     3901 cttacgattt cggatttcct gtacacgaca tcggcacatc ctgtaaatca gtgtgtcgcc
     3961 ggaggggtgc tcgaaaatgt gtttgtttat acgtgagtaa aggcgatggt tgagtgcttt
     4021 ttccagggca tccaggccgc cattttcagg aagacctatc aggctcttga tatgaaaggc
     4081 ctccagggga gagaaacgac tccagcaaat gtctgtacat cttttagctg taaacatgtc
     4141 gaaagttttc tcaacgcttt gaaaccagac cccatcggca gctagccagt tgatggagac
     4201 ggcggtgaga atttcctgca gatctttttc tgatttttca agaagctgct ttgggacgcc
     4261 tttagggtcg acctcaaacc ccagtaactt tccaaggcgt ttcatcatta ttggaaaagc
     4321 tgtctgccag gtctgttctt caagttccat ggcactctgt aagccaagct gatattctac
     4381 ttctttaaac cataatccgt aatggagaaa agtaagtctt aaaccatcaa taatttggga
     4441 aacgattctt tccttcctgt taagagtatt tttcttattc atagatgcct cgtaccgtcc
     4501 ctattactgg agcttggatc ctccttctgt gtgtaaagct gaaaggcgta tcctccattg
     4561 cccttcctta cccgcgaagg gtctcccatg aatgatgtaa ataatgtaaa gaaatatgtt
     4621 ttcagacatg ggtgaatggg tatattgccc acgagcagtg tgtccatttt atttccttgg
     4681 gcttgtccga aggaaaatac ttacctacat tttccattat gcttcgcaaa aatgcaaggg
     4741 gaatccccga acctcaccag agaatctgtc gaggctcttc tttctcttcg gggctttatc
     4801 tttgcttgat ccagacaacg aaccgttctg tacccgaaaa gttgacaggg taatgtccaa
     4861 tgctgataaa acgagtgtga ggggtgtcac agaggttgtt gagatgggca atattatcaa
     4921 tgatcccgaa ttttcagcga aagcttcaga aatggtgtca gcccttatta gacaaatcag
     4981 acctattgag tccgtgctga caaagtgaaa cacggataaa tgctgacagg gcagcctcac
     5041 ccagctatcc taaatgcacc gtgagataga aaaagaatta gctctatgga cgcactagcc
     5101 ctctgtaatg ctatccttaa tcctctggca tcatcatcgg ttacaatttg gtgctcaggg
     5161 gtgaggtaac cgcctttcca ccgtccacat ttatccgtga tgaactggtc aagaagcgtg
     5221 tgcttatcaa agtcgagggc ctcacactgg gatgctacaa gctgatattg gaactcatag
     5281 tcaagtatgg ttccaagggg cttccatccg tttgcgcggg ctgtggccag gatgtactgc
     5341 cactcttcct tgttagtgac gtgggtgaca gcttgactgg cactgctgtg gttgattcca
     5401 atgaggaata aaagttcatc gtccatagtt tgttcaataa atgtctccag tcagtagatc
     5461 attgataccc aaatctaata aaatattttg tatttttcca ctattaattg caaatttttt
     5521 tgaaataaca ggcaaggcga tcaacagggc taaaaacata actgctcttt ataagaaaaa
     5581 cagaatgagg caaaaaaaga tgagttatca ggtgaaacgg atggggggca ggaatggctg
     5641 cattccccat ccttccatac taaaattatc agggtgacaa aaagttcttt cgttatttat
     5701 ccactgtggc gagacattca atctcgacct tggctcccaa tgcgaggcca ttcgcgccta
     5761 tggcgcttct ggcaggtaac ctatcctttt taaaataagt cacatagact tcattcatta
     5821 agggccattc cttcatatca gctatcatga ctgtacactt gaacacattg tccatggatg
     5881 agccatattt ttcgagagta gcctttatat tgtcgaatgc ctgttttgtt tcagctttaa
     5941 taccgccggg tacaaccttc atagtggtat aatcaacgcc gatctccccg gacaggtaaa
     6001 ggatatttcc tacgcgcgcc gcatcagaga agggaagggc catgggtcga ggagtcttaa
     6061 agtattcgat cttgctatcc tgttttcctg cctgctgagc atatactgtg aatgaacaca
     6121 caaaacaaaa acagattact gctgcaataa tatggatctt cctcatgttc taacctcctt
     6181 tttatttgaa tttgtcaaat cttaaaccaa ctgattgccc ggtttcaagc catattttat
     6241 tctgactgtt ataagcttcc aataaaaggt taaatatgtt ttatatctcc tgaggcgtga
     6301 gaaaggctac caggcaacat gagaaatctg gtataatcga atgttgcaat atggcgtcaa
     6361 caaggaggaa atttcgtgaa aataggtggg tttgaattac atgatccagt tccggagtgc
     6421 aatgaatctt atgccatagc tacccttagg ccctggatag atgtgaatag tataggaagt
     6481 cttgtgctaa aagaattgaa gtctcggtac gatgctgtag aattaggaaa actatacaaa
     6541 cccggtcatt tctatgattt caccaggtat aggcctacaa tcgacctcga agaaggtatc
     6601 cgtgacatat ccattcccaa ttcaacgatc cactatgcga gaagggagca ggagagtgat
     6661 ctgctgttgc tccggctcct ggaacctcat gctcacgcag agttctacat cgcctctgtg
     6721 ttgaagcttc tcaaaaggtt taaggtaaag aaatatatcc tgcttggaag catgtatgat
     6781 atggtgccgc ataccaggcc tttactggtc agtggatatg ggatgggcac tggagcagtg
     6841 gaggaattaa aaagaacagc ggtattgccc atcacctacc gcggcccatc aaccatttta
     6901 aacctgatcg caaaagaatc ggctgaactg ggcatcgatg caatcgtctt tatcgtctcc
     6961 ttaccccaat atgtagtaat cgaagaagat ttcatgggga aggtcaggct catggaattg
     7021 ctaaactcgc tctataacat cccagtcgac aaaaaagact ttgaaaaagc actggagcag
     7081 cgcacgtcaa ttgccgaacg ggtggaaaga accccagagg ttaagatggt tctgccacaa
     7141 cttgaaaatg tatacgacat gcggatcaga acgatgaaaa caaaggaagg cccgcaactc
     7201 actccggaaa tagaggagat gctctggaaa accatgggga aggacatcgg aaaggcctaa
     7261 ggattagctt gacaagtgtt tgcaataaag aatcaacaga actcgaggca gtcgttaaga
     7321 aatactggga atggcgactg cgggagtttc cggagttttc aacatggact ggtgacaata
     7381 gatataacca taagtttacc gatctttctt tggaggctat agagagacgc caaagcgacg
     7441 aacgccacat gctggagcag gtaaagaata ttgacccgtc cggattatcc gggcaagatg
     7501 ttctatccca tgcgcttctt ctgtgggact tatcacttgc tgtctcagca caacaatttc
     7561 caccggtcat gcttcttacc caaatttctg gacctcagct tatgtttcct caacttgttt
     7621 cagtaacatc ttttcaaacc ttgactgatt acaacaatta ttgtgcgcgg ctggctgctt
     7681 ttcccgtcta tttggaacag gcggccggcc ttcttcgtca tggcattgaa ctgggttggg
     7741 tacagccaaa tggcccactc tcaggggtgc cggggcagat taacggacag atcgtgtctg
     7801 atatcgaaaa aagcccgttg tatacccctt ttcttagaat gcctgatacg gtgaccgaga
     7861 aagagcgtca taggatgaaa tccgaagcaa ataaactgat aaccgatttg gtatttccgg
     7921 ctatgaaccg cttccgggat tttattaccg atatttacat gccggctgga gacaggtctc
     7981 cgggtgtatc cgctcttcct aacggagagg cttactacgc acattgtgta agagaataca
     8041 cgaccactga ccttcaggtt tcagagattc acaatacagg ggttagggag gtggagcgta
     8101 ttcgtgtttt gatgcatggg gtcatcagag agaccggatt caaggggtca ttgcaagagt
     8161 ttgtagattt tttgaggacc gaccctcaat tctatttctc gaagtcagag gaccttgtgg
     8221 tggcctaccg tgacatagca aaaagggcag atgccgagtt gccggcgctt ttttcagaat
     8281 taccccgact gccttacggt gtgcgtgctt ttcctgattt tgaggcgccc aatcaggtaa
     8341 cagccatgta ttaccccggt gcagccgatg gttcaagagc agggtatttc atggtgaaca
     8401 cataccgacc tgacataagg ccgaaatatg aaatggaaac ccttacactt cacgaagctg
     8461 ttcctggaca ccacctgcaa attgccagag cccaggagct tcgacacctg cctgattttc
     8521 ggcgaaacgg ctcttacact gccttcatcg aaggctgggc tctctatgcc gaaagccttg
     8581 gcaggcaaat ggggttttac tcggatccct atgctcgttt cggacagctc atgtatgaga
     8641 tgtggagagc atgccgtctc gtggttgaca caggaataca cagtcttggc tggtcacggc
     8701 aacggtccat tgattatatt cgggagaaca cagccaagac cgaacaagag atcgccgttg
     8761 aggttgatag gtatattgtg ttacccgggc aagcgctggc atacaaagtg ggagagctcg
     8821 ctatcaggga gttgcgggcc agtgcggaaa aaatgctcgg caagtctttt gacatacgca
     8881 catttcacaa tgcccttctt gacaacggtc cccttccgct gtcgatcctg aaaaatcaga
     8941 tggaagaatg gatcaagggt caagcctgag ccatgaactc catttacaag agtgattacg
     9001 ctattacgat cgaagaaaaa cagtgtcgga atatccattt cacgggtatt ttcggcagcg
     9061 gcatgagtgc cctcgcgcaa tatctgtgtt ttcacggatt tatagtgtca ggttccgatc
     9121 gattactact gagtgaggat gctgtttctt tgaggcagtc tcttgagaga gtagggtgtc
     9181 tgatcgttaa ccaggatggt tccggaataa gcacggatac tgatgtggtt tgcgtttcaa
     9241 cggcaattga agaatcaaat ccggatattt ctgcagccca tgcccgcaat attcctgtta
     9301 ttcaccgctc tgacctcctt gctgcaataa tttcctcaaa aaggaccatt gcaatagcgg
     9361 gtacgagtgg aaaatcgaca gtgacgtcca tgatatttga attcctgaca gcctgtggaa
     9421 aatcaccttc tcttataagt ggcgcatctt tgcgtagact ggaaaacgaa gggatgatcg
     9481 gtaatgcttt caggggtagt tctgatctcc tggttgttga ggctgacgag agtgatggaa
     9541 ccatcgtgaa atatcatact gacgtagcgg tcgttttgaa tgtttccaaa gatcacagga
     9601 gtgtagaaga aatcaaagag tttttttcaa ccctgatatc gaaggcaaaa tgggctgtaa
     9661 caaattctga tgactccatc ttagcctctc ttccggcatc agaaagattt ggccgaaatt
     9721 tactggcctc atggcggcct gatcaggaag agcttctgcc catgtcagtg aagctattcc
     9781 gcaacggcat tgaatatcat ttaccgctgc cgggactgca caatcttgaa aacctcaggg
     9841 ccgcgctctg cgtctgtgag cgtttcggct gtgaagggag tgaccttgca agtgctgtac
     9901 aaaattttca aggtgtggca agacgatttt gtatcactga gacgaaacag aatgtgtacg
     9961 taatcgatga ttttgcccat aacccagcaa agatacgagc ggctgttacc gctgcacggg
    10021 gtatttcaga gcatgtagtt tctgtatatc aaccccacgg gtttggcccg acacggtttt
    10081 tgagggatga atacatcaca acttttcggg ctattttcaa agagggtgac ttcctttatc
    10141 ttctgccgat ctattatgca ggcggtacag cacaaaagga tatttgttcc gaggatatta
    10201 tagcaggtct ctgtgatgtc tccttcaaag ctgaggcagt gatcgatcga gatcagttgc
    10261 tggccagatt ggcaaacaac gtaagaccag gagactgtgt tctcgtgatg ggcgccagag
    10321 atccttccct tcctgccctt gtgaaaagaa taatagaaat tttcgacaat tcaaatagtg
    10381 tgtcataaac cacttctttt tggattcctc ccatagtcct gaaactgctt tggaattgtg
    10441 attgatttga gaaaattctc tttaaaaatg gtacatctat acccgggcga acagggctgt
    10501 gtgtccctat ccctctctct ggcac
//
LOCUS       DAOOMC010000100        10492 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdus sp. isolate AMR_MDS_2685
            NODE_7200_length_10492_cov_5.212801, whole genome shotgun sequence.
ACCESSION   DAOOMC010000100 DAOOMC010000000
VERSION     DAOOMC010000100.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864760
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdus sp. (wastewater metagenome)
  ORGANISM  Syntrophorhabdus sp.
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae; Syntrophorhabdus.
REFERENCE   1  (bases 1 to 10492)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10492)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,729
            Genes (coding)                    :: 2,708
            CDSs (with protein)               :: 2,708
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 7 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10492
                     /organism="Syntrophorhabdus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7200_length_10492_cov_5.212801"
                     /isolate="AMR_MDS_2685"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2699756"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(166..945)
                     /locus_tag="PK125_10205"
     CDS             complement(166..945)
                     /locus_tag="PK125_10205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB38513.1"
                     /translation="MKKLLFVTIFLLVAGVLPVPAMADVQVGISIGIPPPITFAAPPE
                     LVVVPSGPNYVYMVPGTVGLYFYGGYWYRHHGGRWFRAVSYTDPWMPIDVGIVPAPIV
                     VVPPDYILNMPPGYHRIRYNDFHRHWRTWERNRYWHNQRWYRDHSRRHWAGQEFHRPP
                     PGHPGFKGPYRKPGVPGYRGPHDRPGSLGPHGKPGGPGYRVPHGKPGGPGSGGPHPKV
                     YRPEGRVGRPGPQPKAVGGPGGGGPHRKASGPSGPGGPPGR"
     gene            1382..2968
                     /gene="pncB"
                     /locus_tag="PK125_10210"
     CDS             1382..2968
                     /gene="pncB"
                     /locus_tag="PK125_10210"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: protein motif:HMM:TIGR01513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004516 - nicotinate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0019357 - nicotinate nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0019358 - nicotinate nucleotide salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HPB38514.1"
                     /translation="MKPTNQLVNPMLTDFYQITMAYAYWKARVHEEEAVFELFFRKNP
                     FGGEFAIYAGLEEKLRLLEHFHFTEEHIAYLKEKIPHAEKGFFDWLKSVDCSGMKIYA
                     MKEGTVAFPRVPLMRVQGPIAIGQMLETSVLTLTNYPSLMTTNSAKYRLAVGFEKGLI
                     EFGLRRAQGPDGGVSASRYSYMGGFDGTSNVLAGYLFDVPIRGTHAHSYVQSFTGIED
                     LKDHTLEAADGEKHDFVNMVLETRNQLGFNNTNEGELAAFITYAQAFPQGFLALVDTY
                     DTLKSGVPNFICVALSLLRIGYKPIGVRLDSGDLSYLSRGARRMFDAIGNKTDPNLSK
                     CTILVSNEINRAVLTSLGLQGHEIDTFGIGTHLVTCDDQPAMGCVYKLVEARGIPRIK
                     LSQELNKMTIPGKKEAYRLFSADGHSLIDLMIKAGDKPPEPGMRVLCHHPFDPIKRVY
                     VTPASVVPLHHCVWDGKRIYPEIVLKELRDYVLNQLKSTREDHLRDINPTPYKVSVSE
                     QLYNYVYHLWTEESPVRELK"
     gene            complement(3009..4211)
                     /gene="xseA"
                     /locus_tag="PK125_10215"
     CDS             complement(3009..4211)
                     /gene="xseA"
                     /locus_tag="PK125_10215"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HPB38515.1"
                     /translation="MLPDEFSIYSVSEINAEIRHLIGDQFRDVLVEGEVSNFKLYPSG
                     HLYFTLKDNTSMLKAVFFNYQGRHPVSSIGDGVAVICRGRIDVYERRGEYQLIVSAIE
                     PRGLGILQLKFQMLKEKLLKEGLFSPETKKPLPVLPTSIGIITSPAGAAIQDMLKVIG
                     KKFNNMSVLIYPVRVQGDEAAFEIVEGIEHFNRTRSVDVIIVGRGGGSLEDLAPFNDE
                     KVARAIYASDIPVVSGVGHEIDFTIADFVADVRAPTPTAAADLVVQAKADFVDTIKSL
                     RSQLEQGMRTRLERCRLALYKQIMGLKENTELFVSHRMYLDDLLNNLIHGFSTYFTAR
                     KTQLESLAQRLKDLNPENILKRGYSITLRKETGEVVISANQVTQGDELAVRLSRGELG
                     VTVTDKAL"
     gene            complement(4327..5610)
                     /gene="thiC"
                     /locus_tag="PK125_10220"
     CDS             complement(4327..5610)
                     /gene="thiC"
                     /locus_tag="PK125_10220"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: protein motif:HMM:NF009895.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HPB38516.1"
                     /translation="MTNQLTAAKEGIITQAMKVVARAESINVEDLRRSIADGKAVILA
                     NNRHKKFTPKGVGKGLNVKVNANIGTSPEMINVATELEKVQNAREAGADAIMDLSTGG
                     DLNEIRKLIIDNANLPIGTVPIYQAVVETVKKRKTITKLDPDEFFDIIEKQGQDGVDF
                     ITVHCGVTQRSLERLKKQGRITDIVSRGGAFLTTWMIANKKENPLFEQYERLLKIAKK
                     YDMTLSLGDGLRPGSIADATDRAQIEELITLGELAQEAINNGVQVMIEGPGHVPLNLI
                     EANILLQKKLCNEAPFYVLGPIVTDIAPGYDHVTSAIGGALAGYYGADFLCYVTPSEH
                     LGLPSPQDVRDGVMVCKIAAHAADIARGNPKALKLDKTMSKFRKALDWNGQIKCAIDP
                     SKITNFRQERKLKDDVCSMCGEYCAIKVVKDYFKK"
     gene            complement(5607..6902)
                     /locus_tag="PK125_10225"
     CDS             complement(5607..6902)
                     /locus_tag="PK125_10225"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: protein motif:HMM:NF020132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HPB38517.1"
                     /translation="MKTVFSIAGFDPSSGAGISRDLDTFYSLGLHGVAAPTSIVVQGP
                     FGVQDVQAVSYGAFKAILATVQESISVDGIKIGVLLNEQYVEALSNYLPEYPEIPVVV
                     DPVIAAKNGLQLLSDKGLKSLIKLIFPKTMVLTPNLDEASHIIGGTVKTLKDMEACAR
                     SLAATGPQSVIIKGGHLRGEPIDLLFDGYECTTWKRKRIDRIIHGTGCVFSSLLTSFL
                     VHGYPIKEAFLTSESLMDDLVRESYRITEDGYFYISSGLLNTTLAERWKVIQALREAK
                     ERLYLLNPVKCIPETQMNVGYAINSAVGIEDVAAFPGRIGHHDGKICFKGDPLFGASS
                     YVARLILDYMRHVPSMRSAVNIRYDKRRLRTAREKGLNIAFLERGQSLDILLKKTLVN
                     IDNPPDIIYDEGEVGKEPMIRLFSRNPHELLKKMEMIAR"
     gene            7249..7725
                     /locus_tag="PK125_10230"
     CDS             7249..7725
                     /locus_tag="PK125_10230"
                     /inference="COORDINATES: protein motif:HMM:NF024833.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YMGG-like glycine zipper-containing protein"
                     /protein_id="HPB38518.1"
                     /translation="MKGITFLILVSAISLVVTACVSDGQNTQRGAVLGGILGAVAGQA
                     IGHNTTGTLIGAGAGALTGAMVGNIHDQQAMEWRMQQRRPSYPERPYGAYYGAESPPY
                     DPDKPYAGGYRGSSHGQYAETPSGYWADVPGQWVSGKWVPSHKVWVPANPGSSYRR"
     gene            7728..8390
                     /locus_tag="PK125_10235"
     CDS             7728..8390
                     /locus_tag="PK125_10235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB38519.1"
                     /translation="MSFVVAIITLLLLSLSLVPPLDAQNSYTEFERGLNLTESQRKKV
                     GEVRERYIREWQLQRQEALRKRLELNDLRKNPYANRDRIDRTQRELRNIERSWEQSYG
                     QYRSDLSHVLTERQRNQYNQFTEWERRNRSMPQGYRGFGTQRQGPMVREGFQGPTIQR
                     RVPVNPEGLARPGMQGQRPVVPDGPRRTWIQRQGPTAPGKRGFPVQQQGQGRREMRGY
                     GR"
     gene            8380..9957
                     /locus_tag="PK125_10240"
     CDS             8380..9957
                     /locus_tag="PK125_10240"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPB38520.1"
                     /translation="MVDKKTIAFPTLLFAIFFILITITGIIQIKIVRNNVNDLLKNQG
                     EILFNHVKREIDINLEYLNLHDKSPSVITPSFLNIIAYDEAIAEDVYTTLTETTPGNL
                     NDTHLENVLIMDEKGKTMARKGNPRVPGSAIQMLVSKKRETVIAMPTSKVQAILMGVR
                     AKEFIIFATIDEDELERFRRRHITREIIENEGKRFDVSGITVYDPTGELYAWTGERNE
                     HAFVLVRPLNSKFLPRYYMEVFLSRVGADEIFKQTMASFVLILVLLALAGAVSTYATF
                     LLAKKYDKKVKEMERDMEVKDRLVSLGKLASGMAHEIRNPLNAISISIQRLKREFAPG
                     DEKKDEYNRFIDIMRGELGRVDRIVEEFLLSTKSRVPFSSENLHNVVEDVVTILHEKG
                     AMKNVTLINNVEKDIVVECQTERIKQAFYNIILNGIEAVAGKGAVEVLSECVGHHVNI
                     TVKDNGSGIAEAHVHSIFEYYYTTKDKGIGLGLPISYMIVKDHGGDIKVFSEKGKGTR
                     FIITLPFVQQRTEKLES"
     gene            10024..>10492
                     /locus_tag="PK125_10245"
     CDS             10024..>10492
                     /locus_tag="PK125_10245"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HPB38521.1"
                     /translation="MERATILIVEDEETQRSLLSGLLQKEGYTVEEAGNGANAIDLFR
                     TKTIDLVLLDFKLPDMDGLAILKGVKEINPEVEIIMITAYGSIENAVNALKAGASEYL
                     TKPIDLDDLLFKIKKIENKKHLVHENRVLKEALKDRLKSEDFIYTSEKMGEVVS"
BASE COUNT         2882 a         2334 c         2449 g         2827 t
ORIGIN      
        1 ccttgtttcg aaatacagcc gtaaaagaaa aacctgcttt actgttaaga ttgtctttat
       61 aggttacaag caaactgggg gagggcgttg cctcccccag tttgtatgct tcgtaaagaa
      121 attttgtaaa ctttcactct cagagagggc gatacaaact ttcaactacc tgccaggagg
      181 cccacctggt ccgctagggc cagatgcctt tctatggggt cctccgccac ccggccctcc
      241 aaccgctttt ggctgaggcc ctggtctgcc taccctgccc tctggcctat ataccttagg
      301 gtgcggtccg ccgctgccgg gtccacccgg tttaccatga ggtaccctat atcccggtcc
      361 acccggttta ccatgcggtc ccaaacttcc tggcctgtca tggggccccc gatatcccgg
      421 tacgcctggt ttccgataag gccctttaaa tcctggatga cctggtggtg gcctgtgaaa
      481 ttcctgccct gcccaatgac gcctggagtg gtctctatac catctttggt tatgccagta
      541 acggttgcgt tcccaggttc gccagtgacg atgaaaatca ttgtaacgga tcctgtggta
      601 acccggtggc atgtttagaa tgtaatcagg gggtactaca acgattggtg ctgggacaat
      661 gcctacgtcg atgggcatcc atggatcagt gtaagagaca gccctaaacc aacggcctcc
      721 atggtgacga taccagtatc cgccatagaa atagaggcca acagtgccgg gaaccatata
      781 aacgtagttt ggtccactgg gaaccactac cagttctggc ggcgctgcaa acgtaatggg
      841 cggcggaatg cctatgctaa tacctacctg cacgtccgcc attgccggga caggtaaaac
      901 tcctgcaact aacaaaaaaa ttgtgacaaa tagtagcttc ttcacctcag tcctccttta
      961 gttttttcat tactttgatc cataataaac attgtttaat gaattcattt ttcaaacaat
     1021 agtttttgaa gcaatatcta taccgtgtat tctgttttta aaaacctact gaaattactc
     1081 aataataaaa aggtttttga caaaattatg ataacatttt tgtcgttttt tgcaaatata
     1141 ttcgacaaaa gcgtcataaa cggatttctc cactttccgg tttattgctc gtctcgactg
     1201 tgccaagctc tttccaatta acgctctgtg agggctactt tttgattgtg catattgcct
     1261 gtgtctgtta atatatggaa aactaaggaa taatgaccat gcaaagctta tatggtttga
     1321 tttactgcca tttgcctaga atcctgaagg acaagcattg gcataggaag gagtatgatt
     1381 gatgaaacct acgaaccaac tggtaaaccc tatgttaact gatttttatc agatcaccat
     1441 ggcatacgca tattggaaag cacgtgtcca tgaagaagaa gctgtttttg agcttttttt
     1501 ccgcaagaat ccatttggcg gagagtttgc aatttatgct ggtctggaag aaaagctccg
     1561 tttgcttgag cactttcatt ttaccgagga gcatattgct tatctcaagg agaaaatacc
     1621 gcatgcagaa aagggatttt tcgattggtt gaaaagcgta gattgttctg gaatgaagat
     1681 ttatgcgatg aaagagggaa cagtagcctt tccccgtgtt cctctcatga gggttcaggg
     1741 tcccattgcc ataggacaga tgcttgaaac gtctgtgctg acattgacca actatccaag
     1801 cctgatgacc acaaactcag ccaaataccg actggctgtg ggatttgaga aaggtttaat
     1861 agagtttggg ttgcggaggg ctcaaggccc tgatggtggg gtttcggctt ctcgatacag
     1921 ttatatgggt ggttttgatg gaacgagcaa tgtccttgcc ggttatcttt ttgatgtccc
     1981 tatccgagga acccatgctc actcctatgt gcagtcattt acaggcatag aggatctgaa
     2041 agatcatacc cttgaagcag cggatgggga aaaacacgat tttgttaaca tggttttaga
     2101 aaccaggaac caattgggtt ttaacaatac caatgaaggg gagcttgcag catttattac
     2161 ctatgcgcaa gcttttcccc agggtttttt agcccttgta gatacctatg ataccttaaa
     2221 atcaggagtc cccaacttta tttgtgttgc cctcagtctt ttaagaatag gatacaagcc
     2281 aatcggtgtc agactggatt caggagacct ttcttacctt tcaaggggtg caagaaggat
     2341 gtttgatgcc attggtaaca agactgatcc aaatctctct aaatgtacaa ttcttgttag
     2401 caacgagatt aaccgggcag tcctgacttc tctcgggcta cagggtcatg aaattgacac
     2461 gttcgggatt ggtacccatc ttgtcacttg cgatgaccaa cctgctatgg gatgtgtata
     2521 caagcttgtt gaggcaaggg gcataccccg tataaagctt tctcaggaac taaacaaaat
     2581 gaccattcca ggaaaaaaag aggcgtaccg gcttttcagc gcagatgggc acagcctcat
     2641 tgatctgatg ataaaagcag gggacaaacc acctgagccg ggaatgcgcg tattgtgcca
     2701 tcatcccttt gaccccataa agcgtgtata tgtcactcct gccagtgtag ttccacttca
     2761 tcattgcgta tgggatggaa agagaatcta tcctgagatt gtattgaaag agttaagaga
     2821 ctatgtattg aatcaactca agagcacaag ggaggatcac ctgagggata tcaatccaac
     2881 cccgtataaa gtatcagtga gcgagcagct ttataactac gtttaccacc tctggacaga
     2941 ggaatctcct gtgagggaac tgaagtaact ccaaccgggc atgcccctgc atcaatgctg
     3001 gggtggtttc acagggcctt atctgtaaca gtaacgccca gttctccccg ggaaagacgt
     3061 acagctaatt catccccctg tgtaacctga tttgcactga ttacaacctc tcctgtttct
     3121 tttctcaggg ttatactgta gcctctctta agaatatttt cgggattgag atcttttaat
     3181 ctctgggcaa gggattcaag ctgtgttttt cttgctgtga aatatgttga gaacccatga
     3241 atcagattat tgaggagatc atcaaggtac atcctgtgag aaacaaatag ttctgtattc
     3301 tctttgagcc ccatgatttg cttgtagagg gcaagcctgc acctttccag tctggttctc
     3361 atgccctgct caagctgcga gcgcaggctt ttgatggtat ctacaaaatc tgcctttgcc
     3421 tgaacaacca ggtctgcagc ggcagttgga gttggcgccc gcacatcagc aacaaaatcg
     3481 gcaatggtga aatcgatttc atgtcccaca ccagaaacca cgggaatgtc tgatgcgtaa
     3541 attgccctgg ctaccttctc atcattgaat ggtgcgagat cttccaggga tccacccccc
     3601 cgccccacga tgatgacatc tacacttcgg gttctattga agtgttcaat tccctccaca
     3661 atctcaaaag ctgcctcatc tccctgaact cttaccggat aaataaggac agacatgttg
     3721 ttaaattttt ttccaataac cttcagcata tcctgtattg cagcaccggc tggtgaggtg
     3781 ataatgccaa tggacgtggg tagcactggc aatggttttt ttgtctcagg actgaagaga
     3841 ccttctttta aaagcttttc cttgagcatc tggaacttca actgtaaaat tccaagaccc
     3901 ctcggttcga tagcactcac aatgagctgg tattcacccc tgcgttcgta aacgtctatg
     3961 cgaccacggc agattacagc cacaccatcc cctatggaac ttacaggatg tctgccctga
     4021 taattgaaaa agacggcttt caacatggat gtgttatctt taagggtaaa gtagagatgg
     4081 cctgacgggt aaagtttaaa attggaaacc tcaccctcaa caaggacatc tctgaactgg
     4141 tcacctatca ggtgcctgat ttctgcattg atctcggaaa ccgagtagat agaaaactcg
     4201 tcaggtaaca tgtatgaagc tcacagcagg taactcacgg ttcacagctg gtcaaaacgt
     4261 taagattaat agcaactcat ccctgtataa aggctgtttc agggacgaca aaaccagaac
     4321 cctttattat ttcttgaaat agtcttttac gacctttatg gcacagtact caccgcacat
     4381 actgcacaca tcatccttga gttttcgttc ctggcggaaa ttggtaatct tggatgggtc
     4441 gatggcacat ttaatctgcc cattccagtc taaagccttt ctgaattttg acattgtttt
     4501 gtcgagtttt aatgccttgg ggtttcctcg cgcaatatcc gcagcatggg cagctatctt
     4561 gcaaaccatt accccatctc gaacgtcctg gggagaaggg agcccaaggt gctcagaggg
     4621 tgtgacatag cagaggaaat ctgccccata atagccggca agggcgcccc cgatagctga
     4681 tgttacatgg tcataccctg gagcgatatc ggtcacaatt ggcccaagca catagaaggg
     4741 ggcttcgtta cagagttttt tctgcaaaag gatatttgct tcaatgaggt tcaacggcac
     4801 atggcccggc ccttcaatca tgacttgcac cccattgttg attgcttcct gggcgagttc
     4861 gccaagagtt ataagctctt caatctgtgc cctgtcagta gcatctgcta ttgagccggg
     4921 tcggagtccg tcacccaaac tcaaggtcat gtcatacttt tttgcaatct tgagcaatct
     4981 ctcatattgt tcaaagaggg ggttctcctt tttatttgct atcatccatg ttgtaagaaa
     5041 cgcaccgccc ctgctcacta tgtcagtgat tctaccttgt ttttttagcc tttcgagaga
     5101 tctttgagta acaccacagt ggactgtgat gaaatccaca ccgtcctgcc cctgtttctc
     5161 aataatatcg aagaattcgt cagggtccag ctttgtaata gtcttgcgtt tcttcacagt
     5221 ttccacaacg gcctggtaaa tggggaccgt gcctatggga agattggcgt tatcaatgat
     5281 aagcttacgg atctcgttca ggtcgccacc ggtactcaag tccattattg cgtctgcacc
     5341 tgcttctcgt gcattttgta ctttttctaa ttcagtggca acattgatca tttcaggaga
     5401 tgtcccaata ttggcattta ccttgacatt caatcctttt cctacaccct ttggagtaaa
     5461 tttcttgtgt ctgttatttg cgaggataac tgccttgccg tcagcaatac tgcgcctcaa
     5521 gtcttcaacg ttaatgcttt cggcgcgggc aaccactttc atggcctggg ttattatacc
     5581 ttcttttgct gcagtcagtt gattcgtcat cgtgctatca tctccatctt ttttaaaagt
     5641 tcatgtgggt ttcgcgagaa aagccttatc atcggttcct tccccacctc gccttcatca
     5701 tagattatat ccggtggatt gtctatatta acaagggttt ttttcaggag aatatcaagg
     5761 ctctgtcctc tttcaagaaa agcgatattt aggccttttt cccgagccgt tctcagtcta
     5821 cgtttgtcat agcgaatatt tacagctgac ctcatggatg gaacatggcg catgtagtcc
     5881 agaattagcc gggccacata cgaggaagca ccaaacagcg ggtcaccctt gaaacatatt
     5941 tttccatcgt gatgccctat ccttccggga aaagcagcca catcctctat acccactgcg
     6001 ctgtttattg catagcccac attcatttgc gtctcgggaa tgcattttac aggatttaac
     6061 aagtaaagac gctcttttgc ctccctcaat gcctgaataa ccttccagcg ctcagcaagt
     6121 gtagtgttga gaagaccgga ggagatatag aaatacccgt cttcggtgat ccggtagctt
     6181 tccctgacca gatcatccat caagctctcg gaggtcagga atgcctcttt tattggatac
     6241 ccatgaacga gaaagctggt gagaagtgac gaaaaaacac agcctgttcc atggataatc
     6301 cggtcaatgc gttttctttt ccatgttgta cactcatagc cgtcaaacag aagatcgatc
     6361 ggctctcctc tgaggtgccc gccttttatg ataactgact gggggccagt tgcggccagt
     6421 gaccgtgcgc atgcctccat gtccttcagg gtttttactg tgccgccaat tatgtgggaa
     6481 gcctcgtcga ggtttggtgt taaaaccatt gtcttcggaa aaatcagctt tatgagactt
     6541 tttaagcctt tatcgcttaa aagttgcagc ccattttttg cagcgattac aggatccaca
     6601 actacaggta tttccgggta ttcaggaagg tagttcgaca gggcctcgac gtactgttca
     6661 ttcaacaata cccctatttt gattccatcc acagaaattg attcctgtac agtcgccagg
     6721 atcgccttga acgcaccata ggacactgct tgaacatcct gaacgccgaa cggaccctgg
     6781 acgacaatgg aggttggtgc tgcaacacca tggagaccga gagaataaaa ggtatcgaga
     6841 tcccgcgaaa tgcccgcacc tgaggaagga tcgaatccgg ctatggaaaa aacggttttc
     6901 attgaacacc tttgatattc tgtaagaata tagcatattt tgaggccaag ttgtaccctt
     6961 gtctgtttcg acaatactgt cgacaaaata tggaatgatc gacatttgtt gcctttgggg
     7021 attggactac aaaggaaaac atcaatgatt gtgtatcact ggaaggctat tatgtatggc
     7081 atatctgttg cttcaaaaga gatagtatgg atgatgaatg aaagcaggag aagatgatgg
     7141 caagacaaga aatcccctgt atgctgtcaa aaggaggata ttttgagaat ttgtaaaaca
     7201 gggaccttga cagggcccta caaaacattt caaactggag ggaggataat gaaggggata
     7261 acatttctta tattagtttc tgcaattagt cttgttgtaa cagcatgtgt atcggatgga
     7321 cagaataccc agcgaggagc tgtgttgggt ggtatacttg gtgcggttgc tggacaggca
     7381 ataggccaca atacaaccgg tactctgatt ggcgccggcg ccggagctct cacaggagcc
     7441 atggttggaa atatccacga ccaacaggca atggaatgga gaatgcaaca aaggcgaccg
     7501 agttatcctg aaagacccta cggcgcatat tatggggctg aaagcccacc ctacgaccct
     7561 gataaaccat atgcaggagg gtaccggggt tcttcgcatg gacagtatgc tgaaacacca
     7621 tctgggtact gggcagatgt gcccggccag tgggtaagcg gaaaatgggt gccgtctcac
     7681 aaggtttggg taccggctaa ccctggaagc tcgtatagaa gataacgatg agttttgtag
     7741 ttgccataat aactctcctt ctcctctcct taagtttggt gccccctctt gatgcacaga
     7801 actcatatac agagttcgag agggggctca accttactga aagccagaga aaaaaagtgg
     7861 gggaggtgcg ggagaggtat attcgagagt ggcaactcca gagacaggag gcgttgagaa
     7921 agaggcttga actcaatgac ctgcgaaaaa atccttatgc taacagagat aggatagaca
     7981 gaacacaaag ggaattgaga aatatagagc gctcttggga acagtcatat ggccagtaca
     8041 gatcagacct ctcacatgtc ctgactgaaa ggcagcgtaa tcagtataat caattcacag
     8101 agtgggaaag aagaaatagg tcgatgcccc agggttacag aggctttggg acacaacgcc
     8161 aaggacccat ggtgcgggaa ggatttcaag gaccaaccat acaaaggcgg gttcccgtaa
     8221 atccggaggg tttggcaagg cctggaatgc agggacaaag gccagtagtt ccagacggcc
     8281 caagacggac ctggatacaa aggcagggac ccacagcacc tggtaaaagg gggtttccag
     8341 tacagcaaca agggcaggga agacgtgaaa tgagagggta tggtagataa aaaaacgatc
     8401 gctttcccta ctcttctctt tgccatcttt ttcattctca ttactatcac aggcatcata
     8461 cagatcaaaa tagtaagaaa caacgtcaat gacctgctga aaaaccaggg agaaatcctt
     8521 ttcaaccatg taaagcgaga gatagatatc aatctggagt atttaaatct ccatgacaag
     8581 tcaccctctg tcataacccc cagctttttg aacataatcg catacgatga agccattgcc
     8641 gaggatgtct ataccaccct gacggaaacc actcccggaa atctgaatga cacccatctt
     8701 gaaaacgtgc tcatcatgga tgaaaaaggg aaaactatgg cgcggaaagg aaatccaaga
     8761 gttcctggtt ctgcaatcca gatgttggtc tcgaaaaaac gggaaacagt tatagcaatg
     8821 cctacgagca aagttcaggc tattcttatg ggtgttcgtg ctaaggagtt tatcattttt
     8881 gccaccattg acgaagatga actggagcgt ttcagaagac gacatatcac cagagaaatc
     8941 attgagaatg aggggaagcg gttcgatgtt tcggggatta cagtttatga tcccactggt
     9001 gagctttatg cctggaccgg agaaaggaat gaacatgcct ttgtccttgt ccggcctctt
     9061 aattcaaagt ttctccccag atactacatg gaagtttttc tttcgagggt tggtgcagat
     9121 gagattttca agcagaccat ggcgagtttt gtgctcattc ttgttctcct tgcccttgct
     9181 ggcgctgtga gtacctatgc aacatttctt ctggcaaaaa aatatgataa aaaggtaaaa
     9241 gagatggaga gggatatgga ggtaaaagat cgtttggtct ctcttggcaa actcgcatca
     9301 ggtatggccc atgagattag aaaccctctc aatgccataa gcatatccat tcagagactg
     9361 aagagggagt ttgctccggg ggatgaaaag aaggatgagt acaacaggtt catagatatc
     9421 atgaggggtg aattaggccg tgttgatcgt attgtagaag aatttctcct atcaaccaag
     9481 tctcgagtcc ctttttcttc tgagaacttg cacaacgtag ttgaggatgt tgtgaccatc
     9541 cttcacgaaa aaggtgcaat gaagaacgta accctgatca acaacgtgga aaaggatatt
     9601 gtggttgaat gccagacaga acgcataaag caggcgtttt acaatatcat cctcaatggg
     9661 attgaagcag ttgcaggcaa gggggcggta gaggtgttat ccgagtgtgt tgggcaccat
     9721 gttaacataa ctgttaaaga taatggttca ggaatcgcag aagctcatgt acacagtatt
     9781 tttgagtact actatacaac caaagacaaa gggattggac ttgggcttcc catatcgtat
     9841 atgattgtaa aagatcacgg cggagatatt aaggttttta gtgaaaaagg taaaggtaca
     9901 aggtttatca taactttgcc ctttgtccag cagagaactg agaagcttga aagttaacag
     9961 gccaaggctt taatcaacct cgtggccgcg tgatttctca gcttcaaaaa gtttggaggt
    10021 gtaatggagc gggcaacaat actgattgtt gaggacgaag aaacgcaaag gtctctcctt
    10081 tcgggccttt tacaaaagga aggttacact gtagaggaag caggcaacgg agccaatgcc
    10141 attgatcttt ttaggacaaa aaccattgac ttggtgcttc ttgattttaa gctgcccgac
    10201 atggatggtc tcgctatctt aaaaggcgtc aaggaaatca atccagaggt ggagatcata
    10261 atgattactg cttatggaag cattgagaat gcagtaaatg ctctgaaagc aggagcatct
    10321 gagtatctga caaaaccgat tgaccttgac gacctgcttt tcaagatcaa aaagattgaa
    10381 aacaaaaaac acctggttca tgaaaaccgt gtcttaaaag aggctttaaa agacaggctt
    10441 aagtcagagg attttatcta tacaagcgaa aagatgggtg aagtggtaag tc
//
LOCUS       DAOOMC010000101        10092 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdus sp. isolate AMR_MDS_2685
            NODE_7642_length_10092_cov_6.672412, whole genome shotgun sequence.
ACCESSION   DAOOMC010000101 DAOOMC010000000
VERSION     DAOOMC010000101.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864760
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdus sp. (wastewater metagenome)
  ORGANISM  Syntrophorhabdus sp.
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae; Syntrophorhabdus.
REFERENCE   1  (bases 1 to 10092)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10092)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,729
            Genes (coding)                    :: 2,708
            CDSs (with protein)               :: 2,708
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 7 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10092
                     /organism="Syntrophorhabdus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7642_length_10092_cov_6.672412"
                     /isolate="AMR_MDS_2685"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2699756"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(186..377)
                     /locus_tag="PK125_10250"
     CDS             complement(186..377)
                     /locus_tag="PK125_10250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB38522.1"
                     /translation="MARHGGGFKSDKRRKELLRLQKQEEKRQRRFSGKTSKETGAEDQ
                     DEDKLTIDESVSKGSTKEI"
     gene            complement(455..670)
                     /locus_tag="PK125_10255"
     CDS             complement(455..670)
                     /locus_tag="PK125_10255"
                     /inference="COORDINATES: protein motif:HMM:NF014304.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional coactivator p15/PC4 family
                     protein"
                     /protein_id="HPB38523.1"
                     /translation="MLIGKIMTNSRRRVMVTIEDQKGVKVVDLRSYQVINDGELMPTS
                     EGISLAPEKVDAVIDLLREAQRKIAGA"
     gene            815..2503
                     /locus_tag="PK125_10260"
     CDS             815..2503
                     /locus_tag="PK125_10260"
                     /inference="COORDINATES: protein motif:HMM:NF019121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HPB38524.1"
                     /translation="MRMHIGVVHTVGSPCCCAEAVTGGLTALGHDVTTVNSEEIEVRA
                     AELARNCDLVIDHTDTFRGRGIFRAFVRLILEGHGVRLVGSGSRACFTADDKIVSKAC
                     LASAGIITPPGIVIRSKQWKPPSWLRFPLILKPAFEHMSRGVVVAETMVDARSKAAHM
                     LKAFQQPIIAESYIAGRELAVSVLEGPHGLEILPPLEWKIDDEEYGFLSEDFKLEERS
                     GERNDAVKALIPAHLQEQLKNHVGYAFRSLCLRDYARFDLRLSPGGTFYFLEANVTPS
                     LEPLEAFALSAQWAGIDYPSLVDRMLSAALGRYGDSVAEGEAAVTLDLPFGPLELLVP
                     RGVHTPPPSTIQLAGILDIQPGEKVMDLGCGTGILSIAAAKLGARRVVAVDISTKALE
                     ATAHNVRQNGFEKQIETRFGSWYELVGKESKTYHKKERYDVIIATPPQTPGPYPFGPR
                     YGGHDGTQHLFTVIDGASEFLSSQKGRLWLIVLSIANPRAVFERLKVRFKDVTTVCQT
                     GRIFSADEYESLEQGLFQYLLQLRSHGTAEFQDMGGGQYMFYNFFIRASGAKQL"
     gene            2500..3363
                     /locus_tag="PK125_10265"
     CDS             2500..3363
                     /locus_tag="PK125_10265"
                     /inference="COORDINATES: protein motif:HMM:NF016668.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChbG/HpnK family deacetylase"
                     /protein_id="HPB38525.1"
                     /translation="MKSLIINADDLGADEPRNRGIFEAIEAGRVTSVSILVNGNGFEG
                     AMERWKSLGNTGISLGIHINLSEGRPIRKDLNLLTDKDGYFLGKQTAQELLKRKADGK
                     LEREIQKEIKAQIDVLKEYEVPIKHLDGHQHIHIMPAVLPIAVSEASSSKIRWIRIPD
                     EPQPSIRNAMSKADILDEAAIFSSLGTAARSFVMLSEIESCDHFRGLYLKGKLDIIHL
                     EELFQNLPIGLTEFMVHPGRAADSETTGPFTTFSSTEREKELAALLDPVFPILLQKYG
                     IDLTPFPEGFP"
     gene            3360..4457
                     /locus_tag="PK125_10270"
     CDS             3360..4457
                     /locus_tag="PK125_10270"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HPB38526.1"
                     /translation="MKKNLCVLILTPTTLPDISGNAISAERWRQSLIGKGLFVRVIAT
                     TNLDADNLVKNIESTKADVVHAHHVSKAGGLFLDQKIIEQYDGLPLVVSPAGTDILSQ
                     DGQVHNLNTIVARVCRRACIIVTQGEWTANRLATLFPDLKNRILHVPKTYTWFGDDSF
                     DLRKITGWSPKHFVFFLPAGIRPVKRNLETLIAMRSVYELRPHVRLVFAGKPLDQDYA
                     ARFQEELERQSEFAQWIPIIPHGSMRSAYATVDVVINSSSSEGLSNAVLEAIGAGKPI
                     LASDIPGNRWPIIGRENVPACGLLFELSDKNDFIRQALKLVDDGQLRNELNKAGRDRA
                     SKWPGVDAEASGLIYAYEEAIRRQHKRSLNL"
     gene            4592..5329
                     /locus_tag="PK125_10275"
     CDS             4592..5329
                     /locus_tag="PK125_10275"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HPB38527.1"
                     /translation="MNLIGRTALITGAGQGIGQACAEVFAERGANLVLMDKNKRTLPK
                     VADQITEKGGHVKAILIDLTHHRKLHKIIDVVKSQAHIDILVNNAGFDRPGTTMKIGK
                     EQFNAVLGIHVGVPFFLIKSFIQEMRAARWGRIINISSIYGVQGAKGEAAYSTAKAAV
                     IGLTKTAAREAGKDGVTVNAIVPGLIKTPPMIRLPDKYKIPIIEQTLLGRMGEPIEVA
                     KVAAFLASDDSSFITGSTITVSGGWGI"
     gene            5729..6877
                     /locus_tag="PK125_10280"
     CDS             5729..6877
                     /locus_tag="PK125_10280"
                     /inference="COORDINATES: protein motif:HMM:TIGR02032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045550 - geranylgeranyl reductase
                     activity [Evidence IEA];
                     GO_process: GO:0015995 - chlorophyll biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0030494 - bacteriochlorophyll biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="geranylgeranyl reductase family protein"
                     /protein_id="HPB38528.1"
                     /translation="MEKYDVIIVGGSCAGSAAGITLAKAGKNTLIIDKARFPREKLCG
                     GMITEKTAKLLQKTYSVSLEGMIDSRYNAFGVYHADFGRISSYRSDQTLFMVHRYDFD
                     DFFLQEARKAGCTAYLGDEVMEIDRGKIHTASGRVISADYIIGADGCHSTVRKELRGT
                     KNDEKFPLGLEVNIGYDNLKFYVPPEDVFPWVFFGYVKIGYGWVFPKKDFATVGIAGL
                     THYDDKNLLVAFRRLLAAVCVDPSRIARKIKGHPVPLNNHFKTLGAGNILLVGDAARL
                     VEPLTGEGIYFAILSGTFAAQAILTTQGDQVQTYNVLVKRHLRGLFAQARLARTLYFN
                     RWVNTYAMRKMSGNAKWCKYFLELLSGEMDYCRYFKTVLKDRTVYPSL"
     gene            complement(6933..7133)
                     /locus_tag="PK125_10285"
     CDS             complement(6933..7133)
                     /locus_tag="PK125_10285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015863587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HPB38529.1"
                     /translation="MAKGTVKWFNDAKGYGFITQEDGTDVFVHHTAIQGTGFKTLSEG
                     QAVSFDVTDSAKGLQATNVVKL"
     gene            7445..8428
                     /locus_tag="PK125_10290"
     CDS             7445..8428
                     /locus_tag="PK125_10290"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024914.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HPB38530.1"
                     /translation="MKVVFLVQGMSVAASRYRVLQYLPFFHTAGVDTKVFEFPAGVAG
                     WSSLWEPLRDGDIIFVQRKRLPRSVLLALKRLKKKIVYDFDDAVMFKNSLSKNPYSLR
                     RTMSFKRMLHYTDFVVAGNEFLKQEAEKYHSNVKVLPTPVDAERYQEKQISVSDTVNL
                     GWIGDHGSIHYMESYKDVWEAVGRKYKNVVLTIICDTFIETRDISLRKILWNYEREID
                     DLKSLDIGLMPLFDDLWSKGKCGFKIIQYLGVGVPAVCTPVGINRDVIEDNVNGLWAN
                     TQGEWIEKLSVLVEDASLREKMGREGRRKIMDEYTVQVCAPKLLEWIKELA"
     gene            8425..9378
                     /locus_tag="PK125_10295"
     CDS             8425..9378
                     /locus_tag="PK125_10295"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HPB38531.1"
                     /translation="MKRIAFFTHQNPQGYRIQQYFPFLEACGFQVELLTSRMSFLKVV
                     EQIRSADVLYIQRLLFNPLKLSIIRASVKRIVYDFDDAVMHGFRGDSTTRARKFRNMV
                     RHADAVFCGNNFLFQEAKKHRTGAIYYVPTVVNTDEYPVKKHSETEQMVVGWIGSRST
                     LRYLVDLEQLVLNASDTAGVIFKIVADRSLNTKAANVVFEKWAADQEKDMLNSFDIGV
                     MPLRDDIWSRGKCGLKLIQYMASGLPSIAHPFGVAQEIISNGVNGMLRRDIDGWKEAI
                     EVLSSDTQKRAIMGKAARDVIEERYSLRVWGPKVVEIIENL"
     gene            9375..>10092
                     /locus_tag="PK125_10300"
     CDS             9375..>10092
                     /locus_tag="PK125_10300"
                     /inference="COORDINATES: protein motif:HMM:NF018047.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide kinase InaA family protein"
                     /protein_id="HPB38532.1"
                     /translation="MKSYKIQGIEWFVEDEGLIPVLRNVVIPDGSHGRSYVIADYNDS
                     QIFIKSFKEKGLPGFIRNRISSRGKREYRLGNFLLSRSIKTAKPLGYGVSRMGSYIIQ
                     KWIEGRSLLTVFNESEHHSDLLEMLAVFLKILKMHHVRHNDLHSDNILVCDQQLYLID
                     LHKTRIKASFTLLDEASNLSHVLVNIYSYLDSDEKSAFFASYGNTGIQGLVEQEMDRL
                     ANRWIRKKKENTVNLFGRLIH"
BASE COUNT         2840 a         2109 c         2443 g         2700 t
ORIGIN      
        1 tgactcattg aaccacttca cagttccttt cgccatgctg actacctcct tgttaaaatg
       61 tggaagaata cggaactgag ttatgaagag atgacaaaaa tgaaacgctt tgcagctaca
      121 actaacatat gtttccgaaa ccccgataag gttatcgcat aaaaaagaaa aaactacaac
      181 aattattata tttcttttgt gctacccttg gatactgatt cgtcaatagt gagtttgtcc
      241 tcgtcctggt cttcagcgcc tgtctctttg ctggtctttc cgctgaacct ccgctgcctc
      301 ttttcttcct gtttttgcag gcgtaatagt tccttacgcc tcttgtcgct cttgaagcct
      361 ccgccatgtc ttgccaaggt tacctcctca atctttctac caccggtctc tgattcattt
      421 aaccagtgat tgagaataat gggtaagaaa cagtctaagc tcctgcaatt ttcctttgtg
      481 cttcccggag aagatcgatg acagcatcaa ccttctcagg agcgagagaa atgccctcag
      541 atgtaggcat caactctcca tcattgatta cctgatacga tcttaaatcg acaaccttta
      601 cgcccttttg atcttctatc gttaccatga ctcttcgcct ggaatttgtc ataatttttc
      661 caatgagcat acaccctcct gtaagagatt acaaaaaact gttgttcttg tacttttgtt
      721 gacggcaggt agataaggcg gctgcgtatc aagggttagt ttaccatata ctcgttcgtg
      781 atgctacttg ttttgcagag aatgtgttat ctttatgcga atgcatatag gcgttgttca
      841 tacagttggt tctccctgtt gctgcgctga ggcagttact ggtggcctca ctgcccttgg
      901 tcacgatgtg accacggtaa actctgaaga aattgaggtt agagcagcag aacttgccag
      961 gaattgcgat cttgtgatcg accacaccga cactttccgg ggtcgtggca tctttcgggc
     1021 atttgtgcga ttgattttag aaggacatgg tgtccgattg gttggttcag gatccagggc
     1081 atgttttact gctgacgaca aaattgtatc aaaagcctgc cttgccagtg ccggcattat
     1141 cacccctcca ggaatcgtga tcagatcaaa acaatggaaa ccaccttcat ggctgaggtt
     1201 tcctctgatt cttaagccgg cgtttgagca tatgagcagg ggggttgttg tagctgaaac
     1261 catggtcgat gctcgctcaa aggcagcgca catgctgaag gcttttcagc agcccattat
     1321 tgcagaatca tatatagcag gaagagagct tgccgtgtct gtccttgagg ggccgcacgg
     1381 gctcgaaatc cttccccccc ttgaatggaa aattgacgat gaggaatatg ggtttctcag
     1441 tgaggatttt aagttggaag aacggtcagg ggagagaaat gatgcagtta aagcattaat
     1501 acctgctcac ctgcaagagc aactcaagaa tcacgtaggt tatgcatttc gttctctatg
     1561 tctgagagat tatgcccgtt ttgatttaag gctgtcacct ggtggaacat tttactttct
     1621 tgaggccaat gtgacgccaa gccttgagcc attagaagca tttgccctat ctgctcaatg
     1681 ggccgggata gactatccat cgcttgttga ccgcatgctc agtgccgcgt taggccgtta
     1741 cggagattct gtggcagagg gtgaggcggc tgtaaccctt gatcttcctt tcggcccttt
     1801 ggaacttctt gtcccccggg gtgtccatac gcctccgcct tcaaccattc agcttgcagg
     1861 gattctggat attcagcccg gagagaaggt tatggatttg ggctgcggaa cagggatact
     1921 ctctattgct gcagcaaaac tgggagcgag aagagttgtt gcagttgaca tcagcacaaa
     1981 agccctggaa gcaactgccc acaacgttcg ccagaatggc ttcgaaaaac aaattgaaac
     2041 ccgcttcggg tcctggtatg aactggtggg aaaagaatct aaaacatacc acaaaaaaga
     2101 gagatacgat gtgattatcg ctacacctcc tcagacacct ggaccttacc cttttggccc
     2161 acggtatgga ggtcatgatg gaacgcaaca tctgtttaca gtaattgatg gcgcatcgga
     2221 atttctttcc tcacaaaaag gcaggctctg gttgatcgtc ctttctattg ccaatccaag
     2281 agcagttttt gagcgattga aagtgcgctt taaagatgtt acaacagtct gtcagaccgg
     2341 ccgaatcttc tcggctgatg aatatgagtc ccttgaacaa ggcctgtttc agtatctttt
     2401 gcaacttcgg tcccacggta cagccgagtt ccaagatatg ggcggtggac agtatatgtt
     2461 ctacaatttc tttatccgcg cttcgggagc aaagcaacta tgaaaagcct gattatcaac
     2521 gcagatgacc ttggtgctga tgaaccaaga aaccgcggga tttttgaagc tattgaagct
     2581 ggcagggtta cgagcgttag tattcttgtg aatggcaacg ggttcgaagg tgctatggag
     2641 cgatggaaaa gtcttggcaa cacagggata tctttaggta ttcacatcaa tctctctgaa
     2701 ggaagaccta taaggaagga ccttaatctt ctcacagaca aagatggata tttcctaggg
     2761 aagcaaactg cgcaggaact cttaaaacga aaagcagacg gaaaactgga gcgggagata
     2821 caaaaggaaa tcaaggcaca aatagatgtt ctaaaagaat atgaagtacc aatcaaacat
     2881 cttgatggcc atcagcacat tcacataatg cctgctgttt tgcccattgc agtatcagaa
     2941 gcatccagta gcaaaataag atggataagg attcctgacg agccacagcc ttcaatccga
     3001 aatgcaatgt caaaagcgga catcctggac gaagcagcaa tattcagcag tttaggcact
     3061 gcagcgcgat ctttcgtcat gttgtcggag attgagagtt gcgatcattt tcgtggcctc
     3121 tatctaaaag gcaagcttga tatcatccac ctcgaagagt tatttcaaaa tcttcccatt
     3181 gggcttaccg agttcatggt tcatccgggc agggcagcag attctgagac cacaggtccc
     3241 ttcacaacct tctcctcaac agagagagaa aaggagctgg cagcccttct cgatcctgtt
     3301 ttcccgatac ttctgcagaa atacggaata gacctgaccc cattcccaga aggttttcca
     3361 tgaaaaaaaa cctgtgtgtc cttattctca cccctacaac gttgcctgac atatcgggca
     3421 acgcaatctc tgctgaacgt tggaggcagt ccctgatcgg gaagggattg tttgtacgtg
     3481 taatcgcaac cacgaatctt gatgctgaca atcttgtgaa gaatattgaa agcactaagg
     3541 cggatgttgt ccatgctcac catgtttcca aagcaggagg cctgtttctt gaccagaaaa
     3601 ttatagagca gtatgatggg ctccccctcg tcgtatctcc tgcagggaca gatatcttgt
     3661 ctcaggatgg acaggtacac aatttgaata caattgttgc ccgggtttgc aggagggctt
     3721 gtattatcgt tacccagggc gaatggacag ccaataggtt ggcaacgctt tttcctgatt
     3781 tgaaaaatag aatcctgcat gtgccgaaaa catatacgtg gtttggagat gacagttttg
     3841 acctccgaaa aataacgggc tggtcaccga agcactttgt tttttttctc cctgccggca
     3901 taagaccggt gaaacgaaac cttgagacgc ttatcgcaat gcgatcagtt tatgaattgc
     3961 gtccccatgt tcgactagtt tttgcaggca agccgctcga tcaggactat gcagcccgtt
     4021 tccaggaaga actggaacgg cagagtgagt ttgcccaatg gataccgatt attcctcatg
     4081 gttctatgcg ctcagcatat gcaacagtag acgttgtcat aaattcatct tcctctgaag
     4141 gactctccaa tgctgttctc gaggctattg gtgcagggaa acctatttta gcatcagaca
     4201 tcccgggaaa taggtggcca atcatagggc gagaaaatgt tcctgcctgt ggacttcttt
     4261 ttgaactatc agacaaaaat gactttatca gacaagcatt aaaactcgtt gatgacgggc
     4321 aactgagaaa tgaattgaac aaagcaggca gagaccgggc ctcaaaatgg ccgggtgttg
     4381 acgcagaagc cagcggactg atttacgctt acgaagaagc cataagaagg cagcacaaaa
     4441 gatctctaaa cctatagctg atagaggagt attgcgttgt ttacaggctg ccattttatc
     4501 tgtgaactgt tcgccatgcg ttttatttca ttgacaatgg aaaacagctt ctgataccgt
     4561 agtgccacag atttttctcc aggagggttt gatgaacctt atcggtcgaa cggcattgat
     4621 tacaggggca ggacagggta ttggccaggc atgtgccgaa gtctttgctg aacgcggtgc
     4681 aaaccttgtt ctcatggata agaataagag gaccctgcca aaggtagccg accagattac
     4741 tgaaaaaggt gggcacgtca aagcgatcct cattgacctt acccatcaca gaaaacttca
     4801 caagatcatt gacgtggtca agtctcaagc acacatagac attcttgtga acaacgctgg
     4861 atttgaccgt ccgggtacca ccatgaagat tggtaaagag cagtttaatg cagtgctcgg
     4921 cattcacgtt ggcgtgcctt tttttctgat taaatcattt atacaggaga tgcgggcagc
     4981 taggtggggc aggatcatca atatcagttc catatatggt gtacagggag cgaagggtga
     5041 ggcagcatat tccactgcca aagccgctgt gatcggattg acaaaaacgg cagcccgtga
     5101 agcaggaaaa gatggtgtaa cggttaatgc cattgtccca ggtctcatta agacaccacc
     5161 gatgatcagg ttgccggaca aatacaagat ccctattatt gaacagactt tgctcggtcg
     5221 tatgggcgaa ccgattgaag tagcaaaggt ggcagctttt cttgcatctg atgattcttc
     5281 attcataaca ggatcgacca taaccgtttc aggcgggtgg gggatatgaa agacagtaaa
     5341 atatgaaaat ctagaagttg gaagtgaaaa gcacaaaagt aaagaaaaac cgtgaaccgc
     5401 caacagtgac ccgtaaagga agatatccag ggagaggtgt aagtcggtct cgcaattact
     5461 gcagccggct tttctagggg ggagtgggga aactctccct ggataagtga ggcatcttaa
     5521 atatttactt ttatagcaac ggccatgcca tggaacagta aacagataat cgattgttaa
     5581 gtatggcttt ttttagtcaa acccggtgat tgctttgaaa gtagctggaa aaatatgtcg
     5641 aaaatgatgg aatatttcga cgataacgtc gcccaggata accgagatac atgctaattt
     5701 gcatcataaa gtgtgatata aaaagaatat ggagaaatat gatgttatta tcgtcggtgg
     5761 gagctgtgct ggttctgcag caggaataac cctggcgaag gccgggaaaa atacactgat
     5821 tatcgataag gcaaggtttc cccgtgagaa gctctgcggg ggaatgataa ccgagaagac
     5881 agccaagctt cttcaaaaaa cctactcagt ttctttagag ggtatgattg actctcgtta
     5941 caatgcattt ggtgtgtatc atgccgattt tggtaggatc tcaagttaca ggtccgacca
     6001 aacactgttc atggtccaca ggtatgattt tgatgatttt ttcctccaag aggccagaaa
     6061 agcgggatgt acagcctacc tgggagatga ggttatggaa atcgacaggg gaaaaattca
     6121 tactgcttcc ggaagggtaa ttagtgcaga ttatataatc ggcgcagatg gttgccacag
     6181 tacagtgcgc aaggagcttc gcggcacaaa aaatgatgaa aaatttcctc ttggccttga
     6241 agtgaacatt ggttatgata atctgaaatt ctatgtgcca cctgaagatg tattcccatg
     6301 ggttttcttt ggctatgtca agatcgggta cgggtgggta ttcccgaaga aagattttgc
     6361 aacagtaggt attgcaggct tgacgcacta tgatgacaaa aacctgctcg ttgccttcag
     6421 gcgtttgctc gctgctgttt gtgtggaccc gagtcggata gcgagaaaga tcaaggggca
     6481 tcctgttcca ctcaacaacc atttcaaaac attgggtgcc ggcaatatac tgcttgttgg
     6541 cgatgcagca aggttagtgg agccgctaac aggggagggg atctattttg ctatcttgag
     6601 cgggactttt gccgctcaag cgatactcac tacacaggga gatcaggttc aaacgtacaa
     6661 tgttttagtt aaaaggcacc tgcgtggtct tttcgcccag gcccgactag caagaaccct
     6721 ctatttcaac agatgggtca atacatacgc gatgcgtaaa atgagtggaa atgcaaaatg
     6781 gtgcaaatat tttcttgaat tactttctgg agagatggat tactgcagat attttaaaac
     6841 agtgctgaaa gatcgcactg tttatccttc actatgataa aggatattgg atgggacaga
     6901 ccctgatgac tcagggcctg taaaattatt ttctaaagct ttacaacatt tgttgcctgc
     6961 aggccttttg cgctatccgt aacatcaaag ctaacagcct gtccttcaga gagggtctta
     7021 aaacctgttc cctggattgc ggtatgatga acaaagacat ctgtgccgtc ttcctgggtt
     7081 atgaagccat aacccttggc atcgttaaac cacttaacag ttccctttgc cacgcgaaaa
     7141 acacctcctt tgcacaattt taaggaatgc ggaactgtgt tatgaagata ctacaatgca
     7201 acggtacgag ctactgcagt aataacagaa acttccgaaa ccttgtaaga cagtatggca
     7261 cagtttaaaa agtagtgcaa tattttttac attatttaaa gtatttgctt tttcacgacc
     7321 ccgtgttatg attttggaca ccggatgaac catttatcaa ttaccattta tcagtcagca
     7381 gtgccaagac agaatcacca cgcaacggat atactgccgc atcctttaaa taaggattga
     7441 tctcttgaaa gtcgtctttc ttgttcaggg tatgagtgtt gcggcaagca ggtacagggt
     7501 tctccagtat ctcccctttt ttcatacagc gggcgttgac acaaaggtgt ttgagtttcc
     7561 ggcgggtgtg gccggctggt catcattatg ggagcctctc agagatggcg acattatctt
     7621 tgttcagcgg aaacgccttc cccgttcagt ccttttagct ctgaaacgtt tgaaaaagaa
     7681 aatagtctat gattttgacg atgcggtcat gttcaagaat tctctctcaa aaaaccctta
     7741 ttcgttgcga agaacgatga gttttaaaag gatgctgcac tacacagatt ttgtagttgc
     7801 agggaacgag ttcttgaaac aggaagcgga aaaatatcac agcaatgtaa aggtactccc
     7861 aacgcctgtt gatgccgaac gttaccagga aaaacagatc agcgtgagcg atacggtcaa
     7921 cttgggatgg attggagacc acggtagcat ccactacatg gaaagctata aagatgtgtg
     7981 ggaagcagtg gggagaaagt acaagaatgt tgttctcacc attatttgtg acacctttat
     8041 tgagacgaga gacatcagcc tgaggaagat actgtggaat tatgaaagag agatcgatga
     8101 tcttaagtcc cttgatatcg gattaatgcc gctttttgat gatctatggt ctaaaggcaa
     8161 atgtggattc aagataattc agtatctcgg ggttggtgtg ccagcagtat gcacaccagt
     8221 cggtattaac cgggatgtaa tagaagataa tgtgaatggc ctgtgggcga atactcaggg
     8281 tgaatggatt gagaagcttt cagtccttgt ggaggatgcg tcattgcggg aaaagatggg
     8341 tagggagggg agaagaaaga ttatggatga atacactgtc caggtctgtg ccccaaagtt
     8401 gttagaatgg attaaggaat tggcatgaaa cggattgcct tttttaccca tcagaatccc
     8461 cagggataca gaatccagca gtacttcccc tttttagagg cctgtggttt tcaggttgag
     8521 ctgcttactt cacgaatgag ttttctcaag gttgttgaac agatacgtag cgccgatgtg
     8581 ctctatatcc agaggctatt gtttaaccct ttgaagctgt ccatcatcag ggcatcggtc
     8641 aaaagaattg tgtatgattt tgacgatgca gtaatgcacg gtttccgagg ggacagcaca
     8701 acgagggctc gaaaattcag aaatatggtc aggcatgctg atgcagtttt ctgtggaaac
     8761 aactttctct tccaggaagc aaaaaaacac aggaccggcg ctatttatta cgttccaacc
     8821 gtggtcaata cagacgaata ccctgtcaag aaacatagtg aaacagaaca aatggttgta
     8881 ggatggatag gaagcaggtc tacgctcagg tacctggtag atctggaaca attagttctc
     8941 aacgcctctg atacggcagg agtgatattc aagattgtgg ctgacagatc cctcaataca
     9001 aaggccgcaa atgtggtttt tgagaaatgg gccgctgatc aggaaaaaga catgctcaac
     9061 agcttcgaca tcggtgtcat gcctctgagg gatgacatat ggtcaagggg gaaatgtggg
     9121 ctcaaactta ttcagtatat ggcatctggt ctgccttcca ttgcccatcc ctttggtgtg
     9181 gcacaagaaa taatatcaaa tggggtcaat ggcatgctga ggcgagatat tgatggctgg
     9241 aaagaggcaa tagaggtact ttccagtgat acacaaaaaa gagccatcat gggcaaagcg
     9301 gcaagagatg ttatcgagga aagatactct ctcagggtat gggggccaaa agttgtggag
     9361 attatagaaa acctatgaaa tcatacaaga ttcaagggat cgaatggttt gttgaagatg
     9421 aggggctcat acctgtactg agaaacgttg taatacctga tggcagccac gggaggagtt
     9481 atgttattgc cgattataat gatagccaga ttttcataaa atcatttaaa gagaaaggac
     9541 tgcctggttt tataagaaat agaatttcct caagaggcaa gagagaatat aggttgggta
     9601 attttcttct ttctcgttcc atcaaaacag cgaaaccact tggatacgga gtgtctcgga
     9661 tgggttcata catcattcag aaatggattg agggcagaag cctcctgaca gtgttcaatg
     9721 agtctgagca ccattctgac ctacttgaaa tgctagctgt atttctcaag attctcaaaa
     9781 tgcaccatgt tagacataat gatcttcata gtgacaacat tcttgtctgt gatcagcaac
     9841 tttaccttat cgatcttcac aagacgagaa taaaggcatc atttacactg ctcgatgaag
     9901 cctccaatct gtcccacgtt cttgtaaata tttacagtta cttagattct gatgagaaat
     9961 cagccttttt tgcgagctat ggaaacaccg gcattcaggg gttggtggag caggagatgg
    10021 atcggctggc aaatcgctgg atcaggaaaa agaaggaaaa tacggtcaat ctattcggca
    10081 ggcttattca ta
//
LOCUS       DAOOMC010000102        10046 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdus sp. isolate AMR_MDS_2685
            NODE_7684_length_10046_cov_6.456611, whole genome shotgun sequence.
ACCESSION   DAOOMC010000102 DAOOMC010000000
VERSION     DAOOMC010000102.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864760
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdus sp. (wastewater metagenome)
  ORGANISM  Syntrophorhabdus sp.
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae; Syntrophorhabdus.
REFERENCE   1  (bases 1 to 10046)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10046)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,729
            Genes (coding)                    :: 2,708
            CDSs (with protein)               :: 2,708
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 7 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10046
                     /organism="Syntrophorhabdus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7684_length_10046_cov_6.456611"
                     /isolate="AMR_MDS_2685"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2699756"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1575
                     /locus_tag="PK125_10305"
     CDS             <1..1575
                     /locus_tag="PK125_10305"
                     /inference="COORDINATES: protein motif:HMM:TIGR02123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter fused permease subunit"
                     /protein_id="HPB38533.1"
                     /translation="GLIAIILLAELSRRVVGMTFTLVLVGFFLYCWLGQFFPGPLAHR
                     GYELDRIVGHMYMTLEGMFGVPLSVSVSFVTLFVVYGTFMDVAGAGSFWMDLSLAIMG
                     RHAASAGRGAVITTALLGGPQGSGVATTMSVAPVMWPILKRAGYTPNMAAGLIAAGGI
                     GAVISPPLMGAAAFLIMQFLNVSFWDVVIMVTVPTLLYYLGTFFVVELEARKHNFMPP
                     ESSGKNAGQVLKTQGHHLISLIVLVILLGMGRSPQSAAIWAIVAVIVTSFMSRDRNEW
                     LTPKRIILAAIEGAQNMIPVAVLLAGAGVIVGTFTLTGLGLKVSGLIMSLSGGIPTVA
                     LFLALLASMIIGLSLPITATYIMTVVMVAPALVKVGLPMHVAHLLAFYFAVLSEVSPP
                     VGLSPSAAAAVTGGNPFGAMMQSWKYSLPAFLVPFLFSATTVGANLLILDAHLGGFVM
                     ALISGSAALFCLSIAIVGFLRGPIPLWQRALLVVASIDMAFTSIDFSLQGFIPLVIGA
                     AVVVWNITKYRRQQAK"
     gene            1672..2658
                     /locus_tag="PK125_10310"
     CDS             1672..2658
                     /locus_tag="PK125_10310"
                     /inference="COORDINATES: protein motif:HMM:TIGR02122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TAXI family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HPB38534.1"
                     /translation="MKYRNTILGFVFIAFCVALFAVSVSAQQKLSFSIVTGGTGGVWY
                     PLGGAIGSVITQKVPGVEATAEATTAAIDNLKLLVANRAGLAWAYDYHVVWANEGKIE
                     ALRKKFPLRVVMGFYEQPFHIVTKEGTGIKNLMDLKGKRVSTGAPNSGTEEQADYVLK
                     ALGIDWNKEMKREKLGATESVAAMKDGKIDAFFWSGAVPTSSIIDLASTPGLKMVLLP
                     VTGKEAEIIAKAYPGVFHKTKFTKAGYSTLDKDVDAIAITAVLVAMDTFPADKMYQIV
                     SAIFANTTELSAVWKGATKLTPASAVNQMAPEAIKFLHPGSHKYFKEKGAVK"
     gene            2681..3226
                     /locus_tag="PK125_10315"
     CDS             2681..3226
                     /locus_tag="PK125_10315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB38535.1"
                     /translation="MAPLAAMLLPVFLALTPLNVSWAAGTLVQLIHGDTGRSIACNLL
                     QDGETLVLTWKNSLFDLAVTEVYKAGGGLLTQTGVTFAIPGGGDPPRVKPEDVEDLFH
                     TGGPFRADGLSRPFQKIVFRIGEIGNPILNIQGRQIALAEEVGFGGTIVLESRLSMSN
                     EPLPCPIGAIDYGAKKPTQDR"
     gene            complement(3437..3832)
                     /locus_tag="PK125_10320"
     CDS             complement(3437..3832)
                     /locus_tag="PK125_10320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB38536.1"
                     /translation="MAKGTTGKHKKKTGTVKNLSKHQNVERRQGRRFFFFWVPIIIIV
                     LLFFYAFVFDPPRSVGEPISGTVQEDGQERSSGVTEKIYSVVLDDGRIVKLDGFQMSS
                     KESGKRLLVQENVSLLFKRRSFSFVKYIK"
     gene            complement(3886..4359)
                     /locus_tag="PK125_10325"
     CDS             complement(3886..4359)
                     /locus_tag="PK125_10325"
                     /inference="COORDINATES: protein motif:HMM:NF013722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC/PaaZ C-terminal domain-containing protein"
                     /protein_id="HPB38537.1"
                     /translation="MTYRVRGRTFDDFVIDEEIISGARTVTEADVVNFACLSGDFHPE
                     HMNKEYARKGPLGERIAHGLLIISMATGLLNQTGAFEGTTIAILEVTVRFIKAVKFGD
                     TIRAIQKVVQKKETSKHDRGVLTSLITVLNQDNQIVLEADLVALLYRRGFVPMGG"
     gene            complement(4383..5066)
                     /locus_tag="PK125_10330"
     CDS             complement(4383..5066)
                     /locus_tag="PK125_10330"
                     /inference="COORDINATES: protein motif:HMM:NF020475.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1847 domain-containing protein"
                     /protein_id="HPB38538.1"
                     /translation="MKKKNIAQCAECQYPVQERVCTTPGGKASTGCPSASKKALVQKA
                     SNEYRTDSIAEFARQASIQEGECYSEREKRPYVMHPVKPRFIEIVEFAQKMGYRRLGL
                     IFCLGLANEAGIANRLFKSHGFEVVSVACKVGGVPKEEIGIQEEEKIFIGGHESMCNP
                     ILQAMVANEEKTEFNVLLGLCVGHDSLFFKYADAPTTVLAVKDRVTGHNPLAALYTSG
                     SYYAWLNKT"
     gene            complement(5418..5858)
                     /gene="dtd"
                     /locus_tag="PK125_10335"
     CDS             complement(5418..5858)
                     /gene="dtd"
                     /locus_tag="PK125_10335"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: protein motif:HMM:TIGR00256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HPB38539.1"
                     /translation="MRAVIQRVRKSSVEVEGHVTGEISHGLLVFVGIGKNDGENDIDW
                     MIDKIINLRIFEKEGKFDQSLLDVNGDLLLVSQFTLYGDCSKGRRPSFSNAMGVEKAR
                     DLFDAFVTKARKKVTKVETGVFQAHMDVKLINDGPVTLIIESNK"
     gene            complement(5859..7952)
                     /gene="fusA"
                     /locus_tag="PK125_10340"
     CDS             complement(5859..7952)
                     /gene="fusA"
                     /locus_tag="PK125_10340"
                     /inference="COORDINATES: protein motif:HMM:NF009381.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HPB38540.1"
                     /translation="MKKYEPDVIRNIGLFSHGGDGKTSIVEAMLFLAGENNRLGRVDD
                     GSSLMDYEPEEIERKTTISASFACFEWAKNKINLLDTPGDDNFIADAKLCMKVLEGAI
                     IVVSAVDGVKVGTEKVWEYTNEFGIPSAIFVNKMDRERADFERAVDDVKKSFPDKSIL
                     VVQLPMGKEENFKGVIDLLAMKAYVFKNDGSGSFETIDIPQEFAATASAYREKLVETA
                     VEMNDEVMERYLDGQEITEEEVEKCLKEGVWARKITPVLCGAATWNVGIQPLLDSVVK
                     YMPPPTYKAEVEGIDPRNGNKITRKMSASEPFSAYIFKTITDPFAGKLTLFKVYSGEI
                     HPDMTVLNTANDEKERIGQIFWLVGKKQKSAGFASAGDIAVVAKLKAAGTGGTLCDEK
                     NPIKYEEVKLAPPLISFSLHPKAKGDEDKLNMSLARLIEEDKTIRYSRDEQTREFILS
                     GMGQVHIEVIVEKMKRKFNVEVELKEPKVPYKETIKGTTKVQGKYKKQSGGKGQYGDT
                     WLELSPLPRGAGFEFVDKVVGGAIPRQYIPAVEKGIVEAMVQGILAGYPVIDFRATLY
                     DGSYHDVDSSEMAFKIAASMGFKKGMEQCKPTLLEPIMKMEVIVPDENMGDIMGDLNS
                     RRGKIIGVETKGRNQVVKASVPMAEILKYAPDLRSMTGGRGTFTMEFSHYEEVPGQIA
                     EKIIEAAKREKEAEK"
     gene            8262..>10046
                     /locus_tag="PK125_10345"
     CDS             8262..>10046
                     /locus_tag="PK125_10345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB38541.1"
                     /translation="MRLFSRLFYGFTFFIFFLCIPTSPVQGQWIYTYGTNTGESVASL
                     KQTADGGYILAGSKASGLDMDSHSDAWLLKLDQNGSIVWQKSYGGTGNYSAAFAEQTE
                     DGSYVVVGSYNAGHDYDIWFLKLDAHGNVLLQKTYGGLSDDYAVSAKSTLDGGFLIAG
                     NTYSFGAGNRDVWILKVDAGGNVEWEKTYGGSGFDSINAFEKISGGGYIAAGGTYSFG
                     TVNQDVWILKLNGNGEVTWSRTIGDVSYDYANSVQQTIDGGFIVAGFTGTNSSAWIIK
                     LDANGTVEWQKRYSEDGRYYAASIRQTQDRGYIVSGTFDPWYGGLSSPWILKLDELGN
                     IEWMKKYGGKSRDDLLSILQEQDGRYVAAGTALSFGAGSQDALVLKLNENGSVTPCPF
                     GDAFNTTAVVTAVEIKNAPVDPGTSSATTQNTTALASLTNAKQGKVCPFTDDILLKVK
                     PTNKRGGEGTIESWEGFLSCPDTCQAWYVQDFPVTLNAIPGGASTFLGWKPKSLNCPG
                     TYPCSVTMDKRKSVKAIFDGPRRLKIVISCKNKGQGNVMVLPAMDTCPDSCEHEFRIG
                     SEVSLTANPKTDSVFLGWKGRDCKNEPTN"
BASE COUNT         2544 a         2473 c         2225 g         2804 t
ORIGIN      
        1 ggcctcatag ccattatact tttagctgaa ctttcccgcc gggttgtagg catgactttt
       61 accctggtcc tggtaggttt ttttctttat tgctggctcg gtcaattctt cccgggccct
      121 ctggcgcaca gaggatatga gcttgaccgg attgtgggtc acatgtatat gacgctcgag
      181 ggtatgttcg gggtccccct ttcggtgagc gtctcctttg taaccctttt tgttgtttat
      241 ggcaccttca tggatgttgc aggtgcaggc tctttttgga tggacctttc ccttgccatt
      301 atgggtcgac atgctgccag cgccggccga ggagccgtaa tcacaacggc cctattaggc
      361 gggccccagg gcagcggcgt ggctaccacc atgtcagtcg caccagtcat gtggccaatc
      421 cttaaaagag cagggtacac cccaaacatg gctgccggct tgattgctgc cggtggaatc
      481 ggcgcagtca tctcacctcc gctcatgggg gcagctgcct tcctaatcat gcagttcctg
      541 aacgtatcat tttgggatgt ggtgatcatg gtcactgtgc ccaccctgct ctattatctg
      601 ggaaccttct ttgttgttga gctggaagca agaaagcaca atttcatgcc accggagtcc
      661 tctggaaaaa atgccggaca ggtattaaag actcagggac atcacctgat ctcccttatt
      721 gttctggtta ttctgctggg tatgggacgt tctcctcaat ctgcagccat atgggcaatc
      781 gttgcagtga ttgtcaccag cttcatgagc agggaccgca acgagtggct tacaccaaaa
      841 cgaatcattc tggctgccat tgaaggcgcc cagaatatga tacctgtcgc tgtgcttctt
      901 gcaggtgccg gtgtcattgt gggcaccttc acgttaacgg gtcttggatt aaaagtgtca
      961 gggctcataa tgtcactgag tggcggcata ccgacagtcg ctctttttct cgcccttctg
     1021 gcctccatga ttatcgggtt aagccttccc attaccgcaa cttacatcat gaccgtggtt
     1081 atggtagcac ctgcacttgt caaggttggt ttacctatgc acgtggcaca tttgctcgcc
     1141 ttctactttg ctgtcctttc tgaggtctcc ccaccagtgg ggctttcacc ttctgcagca
     1201 gccgctgtca caggcggcaa cccttttgga gccatgatgc agtcctggaa atattccttg
     1261 cctgccttcc tcgttccctt tctcttttct gcaacaacag tgggggcgaa cctcttgatt
     1321 ttagatgcac atctcggtgg atttgtcatg gcgttaataa gtggttcagc agcacttttt
     1381 tgcctttcta ttgcgattgt cggcttcctg agaggcccta tcccactatg gcaaagggct
     1441 ttgcttgtag tggcatcaat cgatatggcg ttcacttcca ttgatttctc tttgcagggt
     1501 tttattcccc ttgtgatcgg tgcagctgtg gtggtttgga atataacgaa atatagaaga
     1561 caacaggcaa aatgaactca tccggcaata tgaatacaga aagaaacaac cggagatttc
     1621 cggaaggttt tagatataaa gcctgtaact ataacactta ggaggtagaa gatgaaatat
     1681 cgcaacacaa tattggggtt tgtctttatt gcattttgcg tggctctttt cgctgtttca
     1741 gtatcagcac agcagaagct ttccttcagt attgtcaccg gtggcactgg aggggtctgg
     1801 taccccctcg gaggtgccat aggaagcgtg atcacacaaa aagtgcctgg agttgaggca
     1861 actgcagagg caacaaccgc ggctattgac aatttaaaac ttctggttgc taaccgggct
     1921 ggattagcct gggcctatga ctaccacgta gtctgggcaa atgagggaaa gattgaagcc
     1981 ctgcggaaaa aattcccgct gcgcgttgtc atgggattct acgaacagcc attccatatt
     2041 gttaccaaag aaggaacagg cattaaaaat ctcatggatc ttaagggcaa aagggtttca
     2101 acgggtgccc cgaatagcgg cactgaagag caggcagact acgtactcaa ggcgctcggc
     2161 atagactgga acaaggagat gaagcgggag aaactgggtg ccaccgagtc tgtagccgcc
     2221 atgaaagatg gcaagatcga cgcctttttc tggagtggtg cagtgcccac ctcatccatc
     2281 atcgacctgg catctacgcc tggcctcaag atggtactct tgcccgttac tggaaaggaa
     2341 gcggaaataa ttgcgaaagc gtaccccggg gtttttcata agacaaaatt cacaaaggca
     2401 ggctacagca ccctcgacaa agatgtggat gcaatagcga ttacagccgt tcttgtggct
     2461 atggacacat ttccggcaga taagatgtac caaattgtga gtgctatctt tgccaataca
     2521 acagaacttt ccgcggtgtg gaaaggtgca acgaaactta caccggcgtc agctgtgaat
     2581 caaatggcgc ccgaagcaat aaagttcctg caccccggct cccataagta cttcaaggag
     2641 aaaggcgcag taaaataaga tgaagtcagg ggttttcccc ttggcgcctt tggcggcaat
     2701 gctccttccg gtattccttg ccctaacgcc gcttaatgtt tcctgggcag cgggtacctt
     2761 ggttcaactg atacatgggg acaccggcag gagcattgca tgcaacctcc tccaggatgg
     2821 tgagactctt gttctcacat ggaagaattc gctatttgat cttgctgtaa cagaagtgta
     2881 caaagctggg ggaggactgc tgacacagac aggtgtaacg ttcgcaattc ctggcggtgg
     2941 agaccctccc cgtgtgaagc ctgaagacgt tgaagatcta ttccatacag ggggaccatt
     3001 cagggcagat gggctctctc gacctttcca aaagattgtg tttcgaatcg gcgaaattgg
     3061 taatcctatt ctgaatatac aaggccgcca gatagcatta gctgaagagg ttggttttgg
     3121 cggtactata gtactggaat caagattgtc catgagcaat gaacccctgc cctgccccat
     3181 tggcgctatt gactacggag caaaaaaacc gacccaggac cgctaacctg aggccagaaa
     3241 aaagtcaaat aatgtagtta caatggccac caggaccata attaccgggt atctaaccaa
     3301 ctacttcatc atgagatatc caggccgaaa tagtccctcg caagtacctt gttactttgg
     3361 attggctgaa aaaaggaagg agacgtaacg acgacattcc ttccctgtta gctgttatca
     3421 gcagtgatca ttacctctat ttgatatatt ttacgaaaga aaaggatcta cgcttgaaaa
     3481 gaaggctcac attttcctga acaagcaaac gcttacccga ctctttagaa ctcatttgga
     3541 aaccatccag tttcactatc ctgccatcgt ccaggacaac tgagtatatt ttctcggtca
     3601 ccccactgga cctttcctgt ccatcctcct gaactgtacc ggagataggc tctccaacag
     3661 agcgcggagg gtcgaagaca aaggcataaa agaacagtaa aactataata atgatgggta
     3721 cccagaaaaa gaagaaccta cgtccctgcc ttctctccac attttgatgc ttggataaat
     3781 tttttactgt tcccgtcttt ttcttatgtt taccagtagt gccttttgcc atttatcatt
     3841 accctctgtg gctcaagcca aaattgatca aatgcataaa tcgaattacc ctcccatagg
     3901 aacaaaaccg cgtctgtata ataatgccac gaggtcagct tcaaggacta tctgattgtc
     3961 ctgattcagc acagtgatga gtgatgtgag gactccccga tcatgcttgc tcgtctcctt
     4021 tttctgaaca actttctgga ttgcccgtat agtgtcaccg aattttactg cctttatgaa
     4081 ccggactgta acctcaagaa tagcgattgt agtgccttca aaagcaccgg tctgattgag
     4141 tagtcctgta gccattgata tgatcaaaag cccgtgggca attctttctc ccaggggtcc
     4201 ttttcgtgca tattctttat tcatgtgctc aggatgaaaa tcaccggaaa gacaagcaaa
     4261 attaaccaca tcagcctcgg tgactgttct ggctccgctt ataatctcct catcaataac
     4321 gaagtcgtca aatgtacgtc cacgcactct gtatgtcatt agcctccccc ctatgccgcc
     4381 agctatgttt tatttaacca tgcataataa ctgcctgaag tgtagagtgc cgcgagtgga
     4441 ttatgaccgg ttactctatc tttaacagca aggactgttg ttggggcatc tgcatacttg
     4501 aaaaaaagcg agtcatgccc gacacagaga ccaaggagaa cattaaactc tgtcttttct
     4561 tcattagcca ccatggcttg caatatggga ttgcacatcg attcatggcc gccgataaaa
     4621 attttctctt cttcctggat acctatttct tctttgggaa cccctcccac cttgcaagcc
     4681 accgatacta cctcaaaacc gtgacttttg aaaagcctat tggcaatgcc tgcttcgtta
     4741 gccagtccca aacaaaaaat caaccctaac ctcctgtacc ccatcttctg cgcaaattcc
     4801 acaatctcta tgaacctcgg tttaacagga tgcataacgt acggtctttt ttctctttca
     4861 gaatagcact cgccctcctg aatggatgcc tggcgggcga actctgctat agagtctgtc
     4921 ctgtactcat tactggcctt ttgaacaagg gctttcttac tggcagacgg acagcccgtc
     4981 gatgcctttc ctcccggggt tgtacagact ctttcctgaa ccggatattg acattcagcg
     5041 cactgtgcta tgtttttctt cttcattaat tcgcctccat tactgacgct tgtgttatct
     5101 cttccgcagt acccacccac tctgtaggca tcatgcagtg ggagccattt ttcaccgttt
     5161 gcaaacaaag aaccagtgaa ctggactggc tctaatccta actcttttgc agtttagatc
     5221 aaaatagttc caggccgtag ttctgcaacc caaattctgg agatgattat aactctgtta
     5281 cagaagagaa acaacaggaa gatcagaaga taggtaaggg gttaaaattg gtaagagaaa
     5341 agagaagaga ctaaactatg ttcaattgtg acctacaggt tttttaacct gttgcattgt
     5401 gttagctggc attattttca cttgtttgat tcaataatga gcgttacagg gccatcattg
     5461 ataagcttca catccatgtg ggcctgaaat accccggttt ccaccttggt aacctttttg
     5521 cgtgcctttg tcacgaaggc atcgaaaagg tcacgcgctt tttcaacccc catggcattc
     5581 gaaaaggagg gacgtctgcc ctttgaacag tcgccgtaga gggtaaactg ggatacaagg
     5641 agaaggtcac cgttcacatc aaggagtgac tggtcaaact taccctcttt ttcaaagata
     5701 cgaaggttta tgatcttatc gatcatccag tcaatgtcgt tttcaccatc attcttgcca
     5761 atgccaacaa aaacaagaag gccatggctg atctcaccgg tcacatggcc ttctacttca
     5821 acagatgatt ttcggacccg ttgtattact gctctcaact acttttctgc ctctttctcc
     5881 cgtttcgctg cttcgattat cttctcagca atctggccag gcacttcttc gtaatgggaa
     5941 aactccatag taaaggtgcc tcggccgccg gtcatggacc gcaaatcagg ggcatatttc
     6001 agtatctctg ccataggcac tgaagccttt acaacctggt tgcgtccttt tgtctcgaca
     6061 cctatgatct ttcccctcct cgaattgaga tcccccataa tatctcccat attttcatcg
     6121 ggcacaataa cctccatctt catgataggt tcgaggagtg ttggcttaca ttgttccata
     6181 cctttcttaa atcccattga tgcagcaatc ttaaatgcca tttctgacga gtcgacgtca
     6241 tgataggaac cgtcatacag ggttgccctg aagtctatta ctgggtatcc ggccaggatc
     6301 ccctgaacca ttgcctccac gattcccttc tcaactgccg gtatatactg cctcgggata
     6361 gcgccgccaa caaccttgtc cacaaactca aacccggcgc ctctcggaag gggactgagt
     6421 tcaagccatg tgtcgccata ctgtcctttt ccgcctgact gcttcttgta ttttccctgg
     6481 accttggttg tccccttgat tgtctcctta tagggaactt tcggctcttt cagctcaact
     6541 tccacattaa acttcctttt catcttttct acgataactt caatatgaac ctgccccatg
     6601 ccagacaaaa tgaactccct tgtttgctca tcccgggaat acctgattgt tttatcctct
     6661 tctatcagcc ttgccagaga catgttgagc ttgtcttcat ctcctttcgc ctttgggtgt
     6721 aatgagaatg aaatcaacgg tggagcaagc ttcacttctt catattttat cggattcttt
     6781 tcatcacaca gggtacctcc agtgcctgcc gctttaagct ttgctacaac ggcaatatct
     6841 cctgccgacg caaacccggc tgatttctgt tttttaccca ccagccaaaa aatctgccct
     6901 atcctttcct tctcatcgtt tgctgtgttc aatacagtca tgtcaggatg gatttcacca
     6961 gagtacactt taaaaagggt aagcttgcct gcaaaaggat cggttatggt cttgaaaata
     7021 tacgcactga agggttcact tgcagacatc tttctcgtaa tcttgtttcc attcctcggg
     7081 tctatacctt caacttcagc tttatatgtt ggcggaggca tgtatttcac aacgctatca
     7141 agaaggggct ggataccaac attccaggtt gcagcgccac aaagcacagg cgttatcttc
     7201 ctcgcccaca cgccttcctt caggcacttc tctacctctt cttctgttat ctcctgtcca
     7261 tccagataac gctccatgac ttcatcattc atctctaccg cagtctcaac aagcttttcc
     7321 ctgtaagcac ttgcagtggc agcgaattcc tgcggaatat caatggtttc aaaactgcct
     7381 gatccatcgt ttttaaatac atatgccttc atggcaagaa ggtcgataac acccttgaaa
     7441 ttctcctctt tccccatagg tagttgaact accaatattg acttgtctgg aaagcttttt
     7501 ttgacgtcat cgactgcccg ctcaaaatca gccctttccc tgtccatctt attcacgaag
     7561 attgctgagg ggataccaaa ctcgttagta tactcccata ccttttccgt ccccaccttt
     7621 acaccatcaa cggcgctcac tacgattatg gccccttcca atactttcat gcagagtttt
     7681 gcatcggcaa taaaattgtc atccccggga gtgtcaagca ggttaatctt gttttttgcc
     7741 cactcaaaac aggcgaatga agcagagatt gttgtctttc tctctatttc ttcaggctca
     7801 taatccatta atgatgaccc gtcatcaaca cggccgagac gattgttttc tccagcaaga
     7861 aaaagcatgg cctcaacaat tgaagtcttg ccatcacctc cgtgagagaa tagaccaata
     7921 tttctgatga catcaggctc atactttttc atgtgaactc ctttcgagat aactcggata
     7981 ttgaaataaa tgttgtgaat tttacctgtc ttgtgtcaaa aaatcaagac attataagaa
     8041 atgacaacat acctctactt atcgcctttc tgtatatcac aaaactgaga ttccagaaca
     8101 gttcagatat ttgatttttt aaaaaaattt cactgcgagt gtatccatgc aagagcctta
     8161 tgctataatt gtatgtacaa cgaggtattg tgtaataaac aagtatttcc agaaaatagc
     8221 ttcttccgaa gcttgatctt tgatgaatgg ggggcccaac tatgagactg ttttcaagat
     8281 tgttttatgg ttttaccttt tttatttttt ttctctgtat acccacatca cctgtccagg
     8341 gccaatggat ctatacatac ggtacaaata ccggtgagtc ggttgcttcc cttaaacaga
     8401 ctgcagacgg cggctacatc cttgctgggt caaaggcatc cggtctcgac atggattcgc
     8461 actccgatgc ctggcttcta aaactcgatc agaacgggtc catagtatgg caaaaatcct
     8521 atggaggtac gggaaattac agtgctgcat tcgctgaaca aacggaagat ggtagctatg
     8581 ttgtagttgg ctcctataat gcaggacacg actatgacat atggtttcta aaacttgatg
     8641 cacacggtaa tgtgttgttg cagaagacct atggtggcct ctcagatgat tatgcagtct
     8701 ccgcgaaatc aacccttgac ggagggttcc tcatcgctgg aaacacttat tcgtttgggg
     8761 ctggtaatcg tgatgtttgg attctcaaag tagacgcagg tggtaacgtg gaatgggaaa
     8821 agacttatgg cggcagcgga tttgattcca ttaatgcctt tgagaaaatt tcaggcggtg
     8881 gatatattgc cgcaggaggt acatactcct ttggaacggt aaaccaggat gtttggattt
     8941 taaaattgaa cggaaatggt gaagtaacct ggtcgagaac aatcggagat gtgagttacg
     9001 attatgcaaa ctctgtgcag cagaccattg atggtggttt tattgtggct ggattcaccg
     9061 gcactaatag ctcggcctgg atcatcaagt tagatgcaaa cgggactgtg gagtggcaaa
     9121 aaaggtacag cgaagatggc cgatattatg cggcgtccat ccgacaaact caggatcgtg
     9181 gctacatcgt gtccggaacg tttgatccgt ggtatggtgg tctctcatca ccatggatac
     9241 tcaaacttga cgagcttgga aacattgagt ggatgaaaaa atacggcggc aagtctcgcg
     9301 atgatctcct ttcaatcctg caggagcagg acggcagata cgtagcggcc ggcacagctc
     9361 tgtcattcgg cgcaggcagt caagatgccc tggtgctcaa gctgaatgaa aacggttccg
     9421 taactccttg tccttttgga gatgcattca atacaaccgc tgttgttaca gcagttgaaa
     9481 tcaaaaatgc acctgtagat cctggtacgt cgtctgcaac aactcaaaac acaacagccc
     9541 tggcttcact aacaaatgcc aaacaaggta aagtttgtcc ttttactgac gacattcttc
     9601 tcaaggttaa accgaccaac aagcgtgggg gtgaaggaac aattgagagt tgggaaggtt
     9661 ttctttcctg ccctgatacg tgtcaggcat ggtatgttca ggatttcccg gtcactctca
     9721 atgcaatccc gggaggagca tcaacatttc ttgggtggaa accaaaatct ttaaattgcc
     9781 ctggtaccta tccttgcagc gtgaccatgg ataaaaggaa atcggtaaaa gccatcttcg
     9841 atgggccccg aaggctcaag attgtgatct catgcaagaa taaggggcag ggaaacgtca
     9901 tggtgcttcc agctatggac acttgccctg acagttgtga gcatgagttt cgcatcggtt
     9961 ctgaagtatc gcttaccgca aacccaaaaa ctgactctgt cttcctcggg tggaagggaa
    10021 gagattgcaa gaatgagccg accaac
//
LOCUS       DAOOMC010000103        10038 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdus sp. isolate AMR_MDS_2685
            NODE_7692_length_10038_cov_5.630772, whole genome shotgun sequence.
ACCESSION   DAOOMC010000103 DAOOMC010000000
VERSION     DAOOMC010000103.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864760
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdus sp. (wastewater metagenome)
  ORGANISM  Syntrophorhabdus sp.
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae; Syntrophorhabdus.
REFERENCE   1  (bases 1 to 10038)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10038)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,729
            Genes (coding)                    :: 2,708
            CDSs (with protein)               :: 2,708
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 7 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10038
                     /organism="Syntrophorhabdus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7692_length_10038_cov_5.630772"
                     /isolate="AMR_MDS_2685"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2699756"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(74..346)
                     /locus_tag="PK125_10350"
     CDS             complement(74..346)
                     /locus_tag="PK125_10350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HPB38542.1"
                     /translation="MTISGILSHIDASPKAWYIRFWSINKMYYGASISIDIALREKTL
                     MAYEFDREYLEEDYGGLHGPLCPVSGVANLKKCYQDRIGESLYSGF"
     gene            complement(793..1689)
                     /gene="cysK"
                     /locus_tag="PK125_10355"
     CDS             complement(793..1689)
                     /gene="cysK"
                     /locus_tag="PK125_10355"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: protein motif:HMM:TIGR01136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HPB38543.1"
                     /translation="MLVDSILEAVGNTPLIRLKTMSSGNVFAKADFLNPGGSLKDRVA
                     KHIIESAEKDGRLKPGMTIIEATSGNTGIGLTLVGVQKGYRVVCVMPENMSEERTKII
                     QAFGGEIITCPAEGSLTACVGKIQEIIKTSPEKYFVADQFGNPHNPEIHYKQTAPEIW
                     EDMNGEIDIFVAGVGSGGTLQGIGKYLKEKNGRIKIVAVEPKNSAALLGKEPGLHQIQ
                     GIGDGFIPDVLDVNLVDMVFTVSDEEAIVTTRRLAREEGLLVGTSSGANVFAALHMDN
                     GYNRVVTILPDRAERYFSTALL"
     gene            complement(1865..2728)
                     /locus_tag="PK125_10360"
     CDS             complement(1865..2728)
                     /locus_tag="PK125_10360"
                     /inference="COORDINATES: protein motif:HMM:NF016143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HPB38544.1"
                     /translation="MRWRDGRESDNIEDRRGMGIPRGAKVGGVGGLGLLVIVLIGMFF
                     GIDPSVILQSVPQTQAPPVSAKRGGQPARNDDQRKFVAVVLAETEDVWQGVFQRAGRR
                     YVPPKLVLYSGAVESACGIAGYATGPFYCPSDHKVYLDLTFFEDMQTRFGASGDFAQA
                     YVIAHEVGHHVQTLLGISRKVREAQSRVGAAERNRLSVRMELQADCFAGVWAHQAHKS
                     RQILEAGDIEEGLNAASAIGDDRIMRRTRGYVVPDAFTHGTSVQRARWFKRGFDQGSI
                     QACDTFRVDQL"
     gene            complement(2778..3239)
                     /locus_tag="PK125_10365"
     CDS             complement(2778..3239)
                     /locus_tag="PK125_10365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB38545.1"
                     /translation="MKEDEPGILELLIRHELAIKQLYERYGVAFTEHKDFWQRLAEDE
                     QRHADVLGTLRPEPSINRTLLQESQIKPQAIKLSIEYVESQAERAQKGNINLLQALSI
                     AKDLESALLEKQFSKLSNVASKEMRAILMDLAIETERHRNAVVEMLNAQKQ"
     gene            complement(3372..4469)
                     /locus_tag="PK125_10370"
     CDS             complement(3372..4469)
                     /locus_tag="PK125_10370"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HPB38546.1"
                     /translation="MQLSGRVRNVRPSGVRKIFDLAAKVKNPINLSIGEPDFDIPDEI
                     KEEGIKWIRAGFNKYTPSGGIPELREKVFCYLKGKGIICDDVIITAGATGGLLLSFLV
                     TLDPGDEVIIPDPYFVLYEYQLILLGARPVYVDTYPDFSLKEEKLRKALTNKTKAILI
                     NSPNNPTGRVYSRQELEMVVAVARERNLLIFSDDIYDRFIFEDSLRNNYLGQLYENTL
                     TIGGFSKTWAMTGWRVGFVAGSKEIIQTMITNQQYVLSSVNSIAQKAALFALDCNTDE
                     LISSYKKRRDLIYEGLKDKYEVVQPKGAYYIFPRVPGDDGDAFVERALENNLFVIPGS
                     VFSARKSNIRISFASNEENVLKGIEILRSMA"
     gene            complement(4470..4706)
                     /locus_tag="PK125_10375"
     CDS             complement(4470..4706)
                     /locus_tag="PK125_10375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB38547.1"
                     /translation="MEIYCLQLGMLTKFDYCVSMNEGLPCRNLIGCWRDRTDVVAFLN
                     KTLTEDELRKTFSGLPKSKIDRIVDIIKSLKKED"
     gene            complement(4857..6206)
                     /locus_tag="PK125_10380"
     CDS             complement(4857..6206)
                     /locus_tag="PK125_10380"
                     /inference="COORDINATES: protein motif:HMM:NF009057.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrpB-like pyridoxal phosphate-dependent enzyme"
                     /protein_id="HPB38548.1"
                     /translation="MNKKIFLNETDIPREWYNIQADLPTPVPPPLHPGTGQPITPDML
                     APIFPMNLIEQEISQERWIKIPHEVLERLYMWRPSPLYRATNLEKFLDTPARIYYKNE
                     GVSPPGSHKPNTAIPQVYYNKIFGIKRISTETGAGQWGSALSFACNQFDLEAKVYMVR
                     VSYNQKPYRRVMMETWGAKCVPSPSPETSAGKKFFEENPEHPGSLGIAISEAVEDAVT
                     SKDTRYALGSVLNHVLMHQTIIGLEAQKQMAMINEYPNVIIGCCGGGSNFAGLSFPYV
                     RDKIYGRDLQVIACEPTSCPTMTKGPYVYDFGDTAKTTPLLPMHSLGSGFIPAGIHAG
                     GLRYHGMAPLVSRCITDGLIEARAFNQLETFAAGLTWARTEGFIPAPETNHAIASVIE
                     EAKRAKEEGKEKVILFNWSGHGIIDLAAYDAYLAGKLTDYEMPSEEIERLVCDLKRC"
     gene            complement(6650..8230)
                     /gene="serA"
                     /locus_tag="PK125_10385"
     CDS             complement(6650..8230)
                     /gene="serA"
                     /locus_tag="PK125_10385"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: protein motif:HMM:TIGR01327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004617 - phosphoglycerate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HPB38549.1"
                     /translation="MKILIADALSDKALDILTSANLSVDVRTGLKAEELQNVIGEYDG
                     VIVRSATKITKDIIAKGDKLKVIGRAGIGVDNVDVQTATEKGIVVMNTPQGNALAAAE
                     HSIGLMFAAARKIAIADRTMKQGKWEKKALMGTEIYGKTLGVIGIGNIGIIVAEKALA
                     LGMKVLAYDIFVAKEFAESKGIELVDLDTLLRESDFIAIHVPLVKETRSLINKETIGK
                     MKKSAIIINVARGPIVNEKDLYEAIQEGRIAGAALDVFDEEPPPKDNPIVCSDRVVCT
                     PHLGASTAEAQDKVAIDIANQIVDFFKNGVIKNSVNAPSVSFDVAKKIAPYLKLAENL
                     GAIVSAITDFPIKQIQIQYMGDIADMDTKILTQGIIKSILSNQIQGVNYVNAPSIAKS
                     RDVKIVESKMGEHEDFTCLLAIMVKGDGKENIAYGTLLGKKEPRLIRVNKIYLEAELT
                     GQMVFMYTNDKPGVIASIGSIFAKHNINIGGMHFGRENVGGLAITLLDVDIKVEDSVV
                     RELTSLQNVLSVKMINLA"
     gene            complement(9585..9788)
                     /locus_tag="PK125_10390"
     CDS             complement(9585..9788)
                     /locus_tag="PK125_10390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB38550.1"
                     /translation="MALDEPKENDEVFNEGGLTFLVNKDLLNSVKPINVEFIETSNGS
                     GFSVTSALSSKGKTDNCCGTCSC"
BASE COUNT         2664 a         2513 c         1889 g         2972 t
ORIGIN      
        1 gtacactttt atttactttt acatctgtga taatagtctt agtctgtgag ttagcaccac
       61 aaattctgtt ccttcaaaaa cctgaatata acgattcacc aattctatcc tgataacatt
      121 ttttcagatt tgcaacccca gagacagggc acaaaggccc gtgcagacca ccataatcct
      181 cctcaaggta ctcgcggtca aattcgtatg ccatgagggt tttttctcgg agggcaatat
      241 ctattgaaat tgatgcacca taatacatct tatttataga ccagaagcga atataccatg
      301 cttttgggct tgcatctatg tggctgagaa tgccagaaat cgtcatgcgc ctcccattgg
      361 ccccggacag aaaaccggct aacccttgtg tggcgcacat cggcaattgt cttgggcatt
      421 gatagcaact atttccatgt tattttaccc cgcttatcac aaaggcctac aacctctatt
      481 tcccaggttt gtctgttcag tggccatgga ttgcctcaac ccaaaaaaat gggtgttcgt
      541 tgatctctga gtctgctcat aatattcatc tctttttgta ctatatgcgc acattaagta
      601 gccctatcct gtttgtcaat agatcgagaa tttggaattt ttattattaa catggtttgt
      661 ttggcacctt aaagggcggc aatgaaaaca cacaacttaa aaatatatgg ttaaattagc
      721 cgtttcactg acatgtgtgt ggctcttaag ccggaaagaa attaaactaa tcaacagcga
      781 agatcacgac aatcagagca acgcggtact aaaatatctc tctgcacgat ccggcaggat
      841 cgtgaccacc ctgttatatc cgttatccat gtgtaatgcc gcaaaaacat ttgcccccga
      901 agatgttcca accagcagcc cttcctcacg ggccagcctg cgggttgtca cgatggcttc
      961 ctcatcagaa accgtgaata ccatatcaac aaggtttaca tccagtacat ctggtatgaa
     1021 tccatcaccg attccctgga tttgatgaag ccctggttct ttccctagaa gggctgcgct
     1081 atttttgggc tccaccgcaa cgatttttat ccgtccgttc ttttccttta aatacttccc
     1141 aatcccctga agagtccctc cgctaccaac tcctgcaaca aaaatgtcaa tctctccgtt
     1201 catatcctcc cagatttcag gagcagtctg cttgtaatga atctcagggt tatggggatt
     1261 gccgaactga tcggcgacga agtatttctc cggagatgtt ttgataattt cttgaatctt
     1321 gcccacacat gctgtcaggc tcccttcagc cggacaggtt attatttcac ctccaaaggc
     1381 ctggataatt tttgttcttt cttcactcat gttttccggc atgacacaga caacacggta
     1441 gcctttttga acaccaacca gggttaaccc tatacctgta tttcctgagg ttgcttcaat
     1501 gattgtcatt cccggcttta gtcttccatc tttttctgcc gattcgatga tgtgtttggc
     1561 tacacgatcc ttcagactcc ctccagggtt taagaaatct gccttggcaa atacattccc
     1621 agaagacatt gttttcagtc tgatgagggg ggtatttcct accgcttcaa gaattgaatc
     1681 tacaagcata cctttctcct gcatcaatta accatataag agcacacaca agattttaga
     1741 ccacttttct ctcatctgta agtgttgctc atgcaatatc atactgcgaa aaatgaccct
     1801 tgtccaggtc aggtcaaaca tgttatgaac ccaaaagttt gttggaaacc tatgcaaatc
     1861 cagatcagag ctgatctact ctgaaggtat cacatgcctg aatactgcct tgatcaaacc
     1921 ctcttttaaa ccaacgcgcc ctctgtacag aagtgccgtg ggtaaaggca tcagggacaa
     1981 cataacctcg tgtccgtctc atgatcctat catctccaat ggcacttgct gcgttgagcc
     2041 cttcttctat gtccccagct tccagaatct gtctgctctt gtgtgcctga tgggcccata
     2101 ctcctgcaaa acagtcagcc tgcaattcca ttcttacaga aagcctgttt ctttcagcag
     2161 caccaacccg cgactgggct tcccgcactt ttcgggagat gccaagaagg gtctgcacat
     2221 gatgcccaac ttcatgggct attacgtaag cttgtgcaaa atccccggat gcgccgaatc
     2281 tggtctgcat atcttcaaag aaagtcaaat ccagatagac cttatgatct gacgggcagt
     2341 agaagggtcc ggttgcgtat cctgcaatgc cacatgctga ttcaacagcc cctgaataga
     2401 gaacaagctt tggcggcaca tatctcctcc cggcccgctg aaaaactccc tgccacacat
     2461 cttcagtctc tgccagtacc acagcaacaa atttgcgttg atcatcgttt cgggccggtt
     2521 gtcctccacg ttttgcagag acaggcggcg cctgtgtttg cggtacactt tgaaggataa
     2581 ctgacgggtc aataccaaaa aacattccaa tcaagacaat caccaataac cccaacccgc
     2641 cgacaccacc aaccttggca ccccggggta tacccattcc cctgcggtct tcaatgttgt
     2701 cactttctcg gccatctctc catcgcattc tgtttcccct cttagcttct tggactcgtc
     2761 aatgtatttc attgaactta ctgcttctgg gcatttagca tctcaacaac tgcgttccta
     2821 tgcctttctg tctcaattgc aagatccatc aatattgccc tcatctcttt tgaagcaaca
     2881 ttgcttaact tagaaaattg cttttcgaga agcgcacttt caagatcttt ggcaattgag
     2941 agtgcttgca gtaaattaat gttgcccttt tgtgctcttt cagcttgact ctctacatac
     3001 tcgatcgaca acttgatcgc ctgaggttta atctggcttt cctgcaacaa cgtcctatta
     3061 atgcttggct caggcctgag agttccaagc acatctgcat gtctctgttc atcctctgcg
     3121 agacgctgcc agaagtcttt atgttccgta aaagccaccc catatctttc gtaaagttgt
     3181 ttaatcgcta actcgtgcct gatcagcaac tcgaggatac ctggttcatc ttctttcatt
     3241 cactctcctc cacacaaccg tatggtaaat aacgaattac ggatgacttg agacaaaata
     3301 ttacacccat caggagagtg cggcaagtgc tttttgtttc aaccagacaa taaatcagcc
     3361 cttatttgag cttatgccat gcttcttagt atctcaatac cttttaaaac attttcctcg
     3421 ttagatgcaa aagaaatgcg aatatttgat ttccgtgcag agaatacact gccaggtatg
     3481 acaaaaaggt tattttccag tgctctttcc acaaaagcgt cgccatcgtc accaggaact
     3541 ctcggaaaga tataataggc gccttttggt tgaacaacct cgtatttgtc cttcaacccc
     3601 tcatatatta agtctctccg ctttttatac gaggaaatca attcatcagt attgcagtca
     3661 agggcaaata atgcagcctt ctgggctatc gaattgacac tgctgagaac gtattgttga
     3721 ttggttatca tggtctgaat tatctccttc gaaccagcaa caaaacctac tctccagccg
     3781 gtcattgccc atgttttaga gaagccacca atagtgagtg tgttttcata aagctgtcca
     3841 agataattat ttcgtaaact atcctcaaag ataaatctgt cataaatatc atcagaaaaa
     3901 atgagaagat ttctctcccg ggcaacagct acaaccattt caagttcttg cctcgaatat
     3961 accctgcctg tcgggttgtt tgggctattg atcagtatag cctttgtttt attcgttaat
     4021 gccttcctca gtttttcttc cttaagactg aaatcaggat atgtatcaac atatacaggt
     4081 cttgcgccaa gcaaaataag ctgatattca tacaaaacaa agtaaggatc aggaattatc
     4141 acttcatcac ccggatctaa ggttaccagg aaagagagga gtagcccccc tgtcgctcca
     4201 gcggtaataa taacatcatc acagattatc cctttccctt taagatagca aaataccttc
     4261 tctcttaatt ccggaatgcc accagagggt gtatacttat tgaatcctgc ccttatccac
     4321 ttgattccct cctcttttat ttcatcagga atatcaaaat caggctcccc tatgctgagg
     4381 ttaattggat ttttaacctt ggctgccaga tcaaagattt ttcttacccc tgatggcctc
     4441 acatttctta ctctccctga aagctgcatc taatcctcct ttttcagaga cttgattata
     4501 tcaacgatgc gatctatttt tgacttcgga agccctgaaa aagtttttcg cagttcatct
     4561 tcggtcaggg ttttattgag gaatgccaca acatcggtcc tatccctcca acagccgatc
     4621 agattcctac atggcaaccc ttcattcata gaaacacagt agtcaaactt cgtcaacata
     4681 ccaagttgaa gacaataaat ttccatatcg ctccttgcag attaagttct ccttattaag
     4741 atcatcattt tgcttgggag gccacaagtt ttgaggttca tttccagcct ccgaagattt
     4801 aaagtgctca tgtttttaac cttgaacctt tagctttaaa cctttcacct tgttttttaa
     4861 cacctcttca ggtcacacac aaggcgctct atctcctcag aaggcatctc atagtctgtc
     4921 aacttaccgg caagataggc atcatatgct gcaaggtcga tgatcccgtg tccactccaa
     4981 ttaaagagaa ttaccttttc cttaccctcc tcctttgccc tctttgcctc ttcgattaca
     5041 gatgcaatag cgtggttcgt ttcaggtgca ggaataaatc cttctgtcct tgcccaagtc
     5101 agacctgcag caaatgtctc aagttggtta aacgcccttg cctctatgag gccatcagtg
     5161 atacacctgc tcacaagcgg tgccataccg tggtatctca aaccaccggc atgaatgccg
     5221 gcgggaataa acccggaacc gagagagtgc atagggagaa gaggcgtagt cttcgcagta
     5281 tcgccaaaat catagacata gggccctttt gtcatagttg ggcacgaagt aggttcgcag
     5341 gctatcacct gcaaatccct tccataaatc ttatctctta cataaggaaa tgaaagcccg
     5401 gcaaagttac tgcccccacc acaacagcct attatgacat ttggatactc gttaatcatt
     5461 gccatctgtt tttgtgcctc gaggccaatg attgtctggt gcatcagtac atgattgaga
     5521 acactcccca gcgcatatcg tgtatcttta gaggtcactg catcctcaac agcttcactt
     5581 atggcgatcc cgaggcttcc aggatgctca ggattttcct caaagaattt ttttccggca
     5641 ctcgtttccg gactcgggct cggtacacac ttcgcacccc aggtttccat cattactctt
     5701 ctgtatggtt tttggttgta gctgaccctc accatatata cctttgcctc aaggtcaaac
     5761 tggttgcagg caaatgacag tgcactcccc cactgaccgg cacccgtttc agtgctgatg
     5821 cgttttattc cgaatatttt gttgtagtaa acctggggaa tagcagtgtt cggcttgtga
     5881 ctgcctggag gactcacacc ttcgttctta taatagatac gtgctggtgt atcaaggaat
     5941 ttctccagat tggtggcccg ataaagaggc gatggtctcc acatgtaaag cctctctaac
     6001 acctcatggg gtatcttgat ccacctttcc tggctaattt cctgctcgat tagattcatt
     6061 gggaatatag gtgcgagcat atcaggcgta attggctggc cagttccagg atgaagcggt
     6121 ggtggtaccg gggttggcag atcagcctga atgttgtacc actctctcgg gatatctgtc
     6181 tcatttaaaa atattttttt gttcatagaa tccccttttt ctcaaaccct ttcatccata
     6241 actaattacc agcaatgttt taaaattgac tgacctggga taacaacgca ctgttgaact
     6301 aaaccaaaag gccatggggt ctgtaaggaa cccatggcct tcgcatcaat gattcactaa
     6361 cggcataaat gctgtcaatg gtactatttg ctacgaggta accaaatcag gcgccctcct
     6421 cacccaccga tgttctctca ggatcaccca ttgcaaccct gaccagctga agacgaacaa
     6481 tcttgccctt gccgcgccag cgttagaaca tctgaaaagc ctctctactc ccccattccc
     6541 cctacataaa tttcatgtgg caatagagta aactccccca ctgtaccact aataggaaat
     6601 aagtatagaa acagcttcca gcattgtcaa gaaaaatgtt gatacacaat tacgcaaggt
     6661 taatcatctt aacactgagc acattctgca aagatgttag ctctcggaca acgctgtctt
     6721 ccaccttaat gtctacatcc aggagtgtta ttgcgagacc gccaacgttc tcccttccga
     6781 aatgcatacc ccctatattg atgttatgtt tggcaaatat actgccaatg ctggcaataa
     6841 ctcctggttt atcattagta tacataaaga ccatttgccc tgtcagctct gcctcaaggt
     6901 atattttgtt aaccctgatt aaccggggtt cctttttacc aaggagcgta ccataggcaa
     6961 tgttttcttt gccgtcacct ttaaccatga tggcaagaag gcatgtgaaa tcctcgtgct
     7021 ctcccatctt cgactccaca atcttaacat cacgactttt cgcaatagaa ggcgcattca
     7081 cataattgac cccctggatc tgatttgaaa gtatgctctt gataataccc tgggtaagga
     7141 ttttcgtatc catatccgca atgtcaccca tatactggat ttggatttgc ttaatcggga
     7201 aatctgtgat agcagaaaca atggctccaa ggttttcagc aagcttcaga taaggggcaa
     7261 tcttcttagc aacatcaaag gagaccgatg gcgcattgac actgttcttg ataaccccat
     7321 tcttgaagaa gtccacaatc tggtttgcaa tatcaatggc taccttatcc tgtgcctctg
     7381 ctgttgatgc tccaaggtga ggcgtgcata ccaccctgtc cgaacacaca ataggattgt
     7441 cttttggagg aggctcctca tcaaatacgt ccaaagcagc acctgctata cggccttcct
     7501 ggatagcttc atagaggtcc ttttcattga caatgggtcc acgtgcaaca ttaataataa
     7561 tagcgctttt cttcatcttt cctatggttt ctttgttaat aagggatcgc gtctctttca
     7621 ccagaggaac atggatggca ataaaatcac tctcccttag gagcgtatcg aggtctacaa
     7681 gttctatccc tttactttct gcaaattcct tggcaacaaa aatatcatac gccaaaacct
     7741 tcattcccag agccagtgcc ttctccgcaa caatgatgcc aatattacca atgccaatga
     7801 cccccagggt cttcccgtag atctctgtac ccatgagggc ctttttttcc cattttccct
     7861 gcttcatagt cctgtcagct attgcgatct tgcgtgcagc ggcaaacatt aggccaatgg
     7921 aatgctcggc cgcagcgagc gcattacctt ggggggtatt catcaccaca atacccttct
     7981 ccgtcgctgt ctgaacatcc acgttatcca cgcctatgcc tgctctccca atcaccttga
     8041 gcttgtcgcc ttttgcgata atatcttttg ttattttggt agcactcctc acaataactc
     8101 cgtcatattc tccgatcaca ttctggagct cttctgcttt gagcccagtc ctcacgtcta
     8161 cagagaggtt cgccgaagtt aagatgtcca gggctttatc cgatagtgca tctgcaataa
     8221 gtatcttcat aatactctcc ttattatggt gttatagttc tttcaaacct taagatattt
     8281 gaaatgattc ctgttcgata tattcatgtg aacccgtata aaaagaattt ggaaaggata
     8341 tcgcagcgtg tcgcaaggac aaaggatacc atttccataa tgccagctcc tgttgttact
     8401 tatgattgga ttttgtattt tgcaaaatgt gatgcaggaa gtcaaggaaa atcatgcgat
     8461 tattgttttc aacctttcat atgcagtaat tctgcctcct caatctgaac acaagaaaga
     8521 tagtcccatt cggtcatgca attacgtttt ttgtagagag atatgatact ggaaaatcaa
     8581 ccttattgag aaccatttcc catcgttgca ccattcatat ccgggagcat gatgaaggtg
     8641 gcctgttaaa cttcaaccag tataagcgta ctctttatag atatattgat gtggaataga
     8701 cagatggagt tgtgccggga tcgtttttca gttcagcggt ttttgactat tttacaaata
     8761 cccaccacga atgatccctc gtctcaccaa actctcgact tcagttccat aaccttttga
     8821 aaacaatgac acaacaaggt catgttcggt tcattattgt gctcacttat cacccctcct
     8881 atcctgcttg gtgcaaagga ttccactatc catagctttg attactcccc tcttcgggaa
     8941 atcagctttg aagaaacgac cccttatcag atttacaaaa aattcctgaa taatcagaac
     9001 aaataacttt aacggctaag taccagacgg accatacggt gagatctgat ttctttttca
     9061 aagtatgagt cacgccattc attaaataat ctgaatccca attttatata taaattttgt
     9121 gtggagtatt attctgaata tgggtcaatc agtcgattat ccaggctcct tcaagggcag
     9181 atgggttaca cacaataatt ctaagggttt ttcccggctg aatatcatca agggaggaaa
     9241 cgccagactt cctgaagacg caggtcaatg cccgctggag aacaggttaa acaagcatgt
     9301 tcggatcatt atcgacaagc ccctaactgt cccctgcctt tttcagtaat caaaagggag
     9361 aaatcataac acccacatct ttgcgtctat gttccatgtc attctgagtt cggagaggtt
     9421 atatccatag caattgagtg ttctcagttg atatagccaa aataggatag ggcacaggat
     9481 atctgcgtta tatgaaaaat caggcttgat agtgaaggag gtaaagataa aaaaaggttg
     9541 aggctacggg gttagattgg cctcaacctt gaaaaaacca cttcttagca gctgcaagta
     9601 ccacagcagt tatcggtttt tcccttactg ctcagggctg atgttactga aaaaccacta
     9661 ccgttacttg tctctatgaa ctcaacattg attggcttca ctgaattgag aagatcttta
     9721 ttaacaagaa atgttaagcc gccttcgtta aaaacctcgt cattctcttt cggctcatcc
     9781 agagccatgc ccagtgaggg ccctgctcac ccgccttccg acatcatgat cctaatggat
     9841 tgaggatcct tggcgtcttt aaggtactct ttaataacct ctactgcctt ctgggatacc
     9901 tggaacatta taaactcctt agcgatagat ttgtcattgt caagtgccaa caacaacagt
     9961 gtaggatatt ttatgtatta ttaccaaatt gtcaactgtt ttcgtcaagt tttcgcaacc
    10021 tcgaattgcc ccatatta
//
LOCUS       DAOOMC010000104         9871 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdus sp. isolate AMR_MDS_2685
            NODE_7911_length_9871_cov_6.563060, whole genome shotgun sequence.
ACCESSION   DAOOMC010000104 DAOOMC010000000
VERSION     DAOOMC010000104.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864760
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdus sp. (wastewater metagenome)
  ORGANISM  Syntrophorhabdus sp.
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae; Syntrophorhabdus.
REFERENCE   1  (bases 1 to 9871)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9871)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,729
            Genes (coding)                    :: 2,708
            CDSs (with protein)               :: 2,708
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 7 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9871
                     /organism="Syntrophorhabdus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7911_length_9871_cov_6.563060"
                     /isolate="AMR_MDS_2685"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2699756"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            203..403
                     /locus_tag="PK125_10395"
     CDS             203..403
                     /locus_tag="PK125_10395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB38551.1"
                     /translation="MIKEIRFTVTGIVREPNAGEWFLGNKGMPICATTDFRTTKFPIL
                     KVEVIEEDTMDVAPKKRQMRVA"
     gene            complement(465..1535)
                     /locus_tag="PK125_10400"
     CDS             complement(465..1535)
                     /locus_tag="PK125_10400"
                     /inference="COORDINATES: protein motif:HMM:NF015948.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HPB38552.1"
                     /translation="MKKVISRMFGDYRVTDMKEFLETSLKSIGLTHQLHHKKILLKPN
                     LVMGKAPHRAVNTHPEVVRAVAELLLDNQCSVSIGDSPGYESTERALKASGIMQVAED
                     LGLTVVPFNKPVKKRLADNISPYQFFTLGEDPALYGAVVNLPKLKTHGMMGLTLGVKN
                     TFGFIHSLEKARWHLKAGQDRALFASILIDICRLVNPTVTILDGVLAMDRDGPSSGRA
                     RRVGVISISTDAFMLDHYIESILGLTSPLPVTRKALEHNLVEEYEVQADAIPEIEDFE
                     IPGLTDTDWTMPAFVKRILKKIFVKKPRLRKKLCKSCSVCCKVCPAEAIELVEKYPVF
                     HYDRCIRCYCCQEMCPEGAIFL"
     gene            complement(1545..2060)
                     /locus_tag="PK125_10405"
     CDS             complement(1545..2060)
                     /locus_tag="PK125_10405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB38553.1"
                     /translation="MRKVISLWVGLTLLLSACALLQPKGQRIVWPEQIRYMEAMCELD
                     MSWQGMNYDGSMSLIMDYPSQLRMELYGPFGNTLMLLKKDNENFLLVTKDEKITDPTL
                     FEDRFGFKIREFMDDIAMIPQKSLAGNGQLTVQKAAYRVLYRSNNKESTMCWENREGS
                     ICIKFLEVKFG"
     gene            complement(2057..3766)
                     /locus_tag="PK125_10410"
     CDS             complement(2057..3766)
                     /locus_tag="PK125_10410"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPB38554.1"
                     /translation="MTLNGFFAPLCGILILMGKALIFVLLILLPLSASAGEKSSSIVD
                     FLKGYRSETRGQYDEAIEQYRAALRSDPDSVQLRAEIALLFLKKGEVAEAEKLLAEAM
                     TINENDRNAMILLAGIYSARGEFLKAKAIYEKCIAANREDTEAYLFLGSLYMSEKRYD
                     EAITVYEKIISYDEGNIMALYYLARLHGEIKSFDKAKQYYQTVIDLKPNFEPALLDMG
                     TLFEIEGNFDKAIEYYRKVVTLDPLNKKVRSRIATIFVKKKQYNQAIYEFEKLSDLDR
                     EDVNLRVKIGLLHLEEERYDEAIREFNIALAANPKNDSVRYYLALSWRGKGDTKKAAQ
                     EFLKIDPKSEEFGSAIKSLAFLYIKSGTLDEGITTMQGYLDRIKDNPDIYMVVALLYE
                     EKQDFKKAIELLGEAHKISAKNTDILYQIGMLYERLGDTDAALRYMDEVLQIDPDYAN
                     ALNFVGYTWADKGINLPEAEEKIKKALDQKPDDAYIRDSIGWVYYKKGEYAKALNELL
                     RAFQSLPDDPTIAEHLGDVYSALNEYVKAIEYFDKALGLEKKEDKKKQIEEKKKALEE
                     RIK"
     gene            complement(3777..4412)
                     /gene="lepB"
                     /locus_tag="PK125_10415"
     CDS             complement(3777..4412)
                     /gene="lepB"
                     /locus_tag="PK125_10415"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:NF021981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HPB38555.1"
                     /translation="MKERHAKQKTKWREYGESLIIALIIAIIVRSFFLQAFKIPSSSM
                     EPTLLIGDHLLVNRLSYVVKVPFTDIVLFHLGSPERGDVVVFRYPLERDKDFIKRVIA
                     TEGETVEIKDKAIFINGKQIEDPWGHFLDKSIVPSYIAPKDNFGPIKVPKDSIFVMGD
                     NRDRSADSRFWGFVHKDDLVGEALILYFSWNRNASNPIDFIRWARFGKLIR"
     gene            complement(4512..6170)
                     /gene="argS"
                     /locus_tag="PK125_10420"
     CDS             complement(4512..6170)
                     /gene="argS"
                     /locus_tag="PK125_10420"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR00456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HPB38556.1"
                     /translation="MVKQQISAIVKKAWENCRQSGFLPPDIDVNIIIEIPREEANGDY
                     STNIAFLLAPKVRKSPTDIAKTLIGNMEFHGVCEKVEVAAKGFINFYVRDDMWRAALR
                     EISDQGLDSLVPDVGEGRKVLIEFVSSNPTGPLHIGHGRGAAVGDVLANIMKRAGYNV
                     VKEYYVNDAGKQIDTLGQSTYARWKELTGDAVPLEEHYYQGDYVKDIASLIVESNVAV
                     PAEKAEAIAFMSKFASDFVLDGIKKDLEEFGVLFDNYFRETRLYESGEVEETLATLKD
                     KGYAYEKDGALWFKTSMFEKDEDRVLIKSDGEKTYFASDIAYHKDKFDRRFDTLIDIW
                     GSDHHGYIPRMRASIEALGRGKDSLKVLLIQFVTLIKDGKPVGMSTRAGQFTTLKEVL
                     NEVGRDAARFFFLMRKSDAHLEFDLDLAKKTTNENPVFYIQYAHARIESIFRVAKDEG
                     IDPASVKTARVDLLTEKDEITLIKGILHFFDVIEGSAKSLEPHRITFYLLDLVGRFHS
                     YYNKTRVIVGDQELTMARLLLLATLQQVIKDALALLGVSAPERM"
     gene            complement(6210..7028)
                     /locus_tag="PK125_10425"
     CDS             complement(6210..7028)
                     /locus_tag="PK125_10425"
                     /inference="COORDINATES: protein motif:HMM:NF012993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HPB38557.1"
                     /translation="MKIAGIQFACSVDKDKNVENALRTIDAAISNGASIICFQELFNL
                     PWFPKDRDEKAFELSESIHGNTVKAMREKAKSAGIVILLPIFEKDKTRFYNSCVVVDE
                     NGEVAGVYRKIHIPDISLWEEKFYFSAGDKGFPVFQTSFGKIGIQISWDNLYPEGTRI
                     LTLKGADIIFAPTACAFKSQHLWQTAISGNAIANGVFIMRVNRAGSEEKQDFYGASFC
                     VNPEGELIGGPTGIADSVLLADVDFDYLQHIRREWPLLRERKPENYKEILSVTP"
     gene            complement(7035..7649)
                     /locus_tag="PK125_10430"
     CDS             complement(7035..7649)
                     /locus_tag="PK125_10430"
                     /inference="COORDINATES: protein motif:HMM:NF013382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HPB38558.1"
                     /translation="MQKAEIQKIYSRYSCFYDIIFSQFFYPRIRLGLEKIGIKNGDRI
                     IEVGVGTGLSLSLYPQSCKVVGIDITRKMLEKAKAKKEKFGFNHVDLFEMDGENICFD
                     DNSFDHAVLPFVASVVPSVEQLMSEVKRVTKKGGKIIIINHLSSKLSFISSMEKLLTP
                     VFMKLGWRTGLSVDTFSNHCNLHIEEVLKKYRLDPWFILHATNR"
     gene            7774..9384
                     /locus_tag="PK125_10435"
     CDS             7774..9384
                     /locus_tag="PK125_10435"
                     /inference="COORDINATES: protein motif:HMM:NF014375.2"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HPB38559.1"
                     /translation="MKVLLIQPPIEDYYDTSIRTYPLSLLYIATKVREICDVSIADLR
                     SNRKPRQIIDHPFPELAPYYRKDRYTPISLFKQYSRFGADLGEIRQRIVFEKPDVVAV
                     SSLFSAYSGEAMDIARCAKEVDDKIITVAGGIHPTLFPEHVLQSPFVDYVVRGEGETP
                     FSVLIEAIKKGTVQSIAGTGGIASKHHGRCIIPDIHIEENLDLVPDRRLVNPSEYRIG
                     KKKYTFFLTSRGCPFHCAFCGRPPFPYRARGLGSVEKEIADCLSLDIQAVDFEDDMLT
                     LDRLRFQNILGLFRGTGLTLSAMNGIYSENLDVATLERMYESGFRRLNFSLVDASASV
                     INMQKRHNTCNFAKLLPYLEASHFLVETHFIIGLPEQRVEHILDTIIFLMGKKLLLGP
                     SIFYLAPNSSVYSRLAPDLQDTNHYFKLMRSSAMVPLNPSIPRDSLYTLMKLVRFVNL
                     VKGLLDGSNVPTRLSDILELPAVTKNDRDRQVLSTLLNEKQLIWFDSSHGTFLPEPHS
                     SAIVRLFFHRAKNKSICGFKTRNRLVVD"
     gene            9686..9865
                     /locus_tag="PK125_10440"
     CDS             9686..9865
                     /locus_tag="PK125_10440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB38560.1"
                     /translation="MRLTTLPWIVKLHRLTPHLIQKITMPVKMTSRKASLLVTWAMCW
                     YVLATVILIVLHACS"
BASE COUNT         2533 a         2432 c         2047 g         2859 t
ORIGIN      
        1 ctctcaccta tcgccttatt ttaaaaccgt gacccgtatc attgctgcct gtttttgtgt
       61 ggctatacta tgatactatg acggattacg gatcaaatgc agcgcctgct gcaggcttga
      121 ttcctctctt ttttacatat tgttattatg ttccctgtct tattaaatac agtaagaaaa
      181 tatgtacaag aggaggtaag ccatgatcaa ggaaatcagg ttcacagtta ccgggatagt
      241 ccgggaacca aatgccggtg agtggtttct cggaaacaaa ggcatgccta tatgcgccac
      301 gactgacttc cgcacaacca aatttccgat cctgaaagtg gaggtcatag aggaggatac
      361 aatggacgtg gcgccgaaaa aacggcaaat gagggttgcc tgaaagaaga ccggcaaaaa
      421 aggccgttac aggcctgatg atgcaggaaa ttatcccaga ggggttacaa aaagatcgcc
      481 ccttctgggc acatttcctg gcagcaataa cacctgatac atctatcgta atgaaacacc
      541 gggtatttct ccaccagttc aatggcttct gccgggcaca ccttgcaaca gaccgagcaa
      601 ctcttacata gctttttcct gagccgcggc ttcttcacga atattttctt caggatcctt
      661 ttcacaaatg ccggcattgt ccagtcagta tccgtaaggc ccggtatttc gaaatcttct
      721 atttcaggga tagcatctgc ctgaacctcg tactcctcca ccaggttatg ttccagcgcc
      781 ttccttgtta caggcagggg ggatgtaagc cctaaaatgc tttcgatata atgatccagc
      841 ataaaagcat ccgtggatat gctgataacc ccaacccttc tcgcccttcc gcttgatggc
      901 ccgtcacgat ccatggcaag aacaccatca aggatagtga ctgttgggtt gacaagccgg
      961 cagatgtcaa tgaggatcga agcaaacaat gccctgtcct gacctgcctt caggtgccac
     1021 cgtgcctttt cgagagaatg gataaagccg aacgtgtttt tcacgcccag agtaaggccc
     1081 atcattccgt gagtcttaag ttttggaaga ttcactactg cgccgtagag agcagggtct
     1141 tcaccaaggg taaaaaactg gtacggcgag atgttatccg caagcctctt ttttacaggt
     1201 ttgttaaagg ggacaacggt aagcccaaga tcctccgcaa cctgcatgat gcctgatgct
     1261 ttcaaagctc tctcagttga ttcgtaaccc gggctatcac ctatggatac gctgcactgg
     1321 ttatcaagaa gaagctcagc cactgcccgg actacctcag gatgagtatt gacagcccta
     1381 tggggtgcct tacccataac caggtttggt ttcaaaagga tttttttatg gtgcaactgg
     1441 tgtgtaaggc cgatgctttt gaggctagtt tcgaggaatt ccttcatgtc tgtgactctg
     1501 tagtcaccaa acatcctgga tataactttt ttcaagaact cctcttatcc aaacttcact
     1561 tcgagaaatt taatgcagat acttccttct ctgttttccc agcacatggt gctttcttta
     1621 ttgttcgatc tgtaaaggac cctgtatgcg gccttctgta ctgttaattg cccattgcct
     1681 gcaagactct tctgtggaat catagctatg tcatccataa attcccttat cttgaatcca
     1741 aaacgatcct caaaaagagt cgggtcagta attttttcat cttttgtaac gagaaggaaa
     1801 ttttcattat ctttctttaa aagcatgagg gtgttcccga agggcccata aagctccata
     1861 cggagctgtg aaggatagtc cattatcaac gacattgagc cgtcgtagtt catgccctgc
     1921 caggacatat caagctcaca cattgcttcc atgtatcgta tttgttctgg ccagacgatg
     1981 cgctggcctt taggctgcag gagtgcgcag gctgaaagca gaagcgtcag ccctacccac
     2041 agggaaataa ccttcctcac tttatccttt cttcaagggc tttctttttt tcctctatct
     2101 gtttcttctt atcttccttc ttttcaagtc ccagggcttt atcaaagtac tcgattgcct
     2161 tgacgtattc attgagggca ctatagacat ctccaaggtg ctctgctatg gtgggatcgt
     2221 cgggaagaga ctgaaatgcc cgaagaagtt cgttcagggc ctttgcatac tcgcctttct
     2281 tgtagtagac ccacccgatg ctgtccctga tgtatgcatc atcaggcttc tgatccaggg
     2341 ccttcttgat tttctcttct gcctcaggaa ggttaattcc tttgtcagcc catgtatatc
     2401 ctacaaaatt aagagcgttg gcatagtcag ggtcgatctg caaaacctca tccatatacc
     2461 gcagggcagc gtcagtgtct cccagccttt cgtaaagcat gcctatctga tacagtatat
     2521 ctgtattctt tgccgagatc ttatgggctt ccccaagaag ttcaatggcc ttcttaaagt
     2581 cctgtttctc ttcgtaaagg agggcaacca ccatgtaaat gtccggattg tcctttatcc
     2641 tgtccaggta tccctgcatt gtggtgattc cttcatcaag ggtaccggat tttatataaa
     2701 gaaaggcaag gctttttatt gcgctgccaa actcctctga tttaggatct attttcagaa
     2761 attcctgggc agcctttttt gtatcgccct ttcctctcca cgacagtgca aggtagtacc
     2821 tgacagaatc gttcttcgga ttggcagcaa gggctatgtt gaattccctt attgcctcat
     2881 catacctctc ctcttccagg tggagcaggc ctatcttaac cctcaggttc acatcctccc
     2941 tgtcgaggtc agatagcttc tcgaattcgt aaatagcctg gttgtactgc ttcttcttca
     3001 caaagatcgt tgcaattctt gaccgcactt tcttgttcag agggtcaagg gtaactacct
     3061 ttcgataata ctcaattgcc ttgtcaaaat taccttcaat ctcaaagagg gtgcccatat
     3121 ccagaagtgc cggctcaaaa ttgggcttta aatcaatgac tgtctgataa tattgcttcg
     3181 ccttgtcgaa tgattttatt tctccgtgca atcgtgccag ataatagagg gccataatat
     3241 tgccctcgtc atagctgatt atcttttcat aaaccgttat cgcttcatca tatctttttt
     3301 cagacatgta gagagagcca agaaaaaggt atgcttcagt atcttccctg tttgcagcaa
     3361 tgcacttctc gtagattgcc ttggctttca ggaattctcc tctggctgaa tatatgcctg
     3421 ccagaagaat catggcgttt ctgtcatttt cgtttatggt catggcttca gcgagaagct
     3481 tttccgcttc tgcaacctca cctttcttca ggaagaggag tgcaatttct gctcttagct
     3541 ggacagaatc agggtcgctg cgcagagctg ccctgtattg ctcgatcgct tcatcgtact
     3601 gccctcttgt ctcgctgcga taacctttaa gaaaatcaac gatggaggat gatttttctc
     3661 ctgctgaagc tgacaacggg agaagaataa gcaaaacaaa gatgagtgct tttcccatga
     3721 gaatcaagat accacacaat ggtgcgaaaa atccattgag ggtcatccgt gcctggctac
     3781 cgtatcagtt ttccaaaccg tgcccaacgg ataaaatcga tagggttgct cgcgttacga
     3841 ttccatgaaa aatagagtat cagagcctcg cccaccaggt catctttgtg cacaaatccc
     3901 cagaacctgc tgtctgcgct tctgtctctg ttatctccca ttacaaagat tgaatccttc
     3961 gggaccttga ttggtccaaa attgtccttt ggtgcgatat aggagggcac aatgcttttg
     4021 tcaagaaaat gcccccaggg gtcttctatc tgttttccat tgatgaagat ggctttatct
     4081 ttgatctcca ctgtctctcc ctcagtagca atgacccttt tgataaagtc cttgtccctt
     4141 tcaaggggat agcgaaagac aactacatcg cccctttccg gactgccgag gtggaaaaga
     4201 acaatatcgg taaaaggtac ctttaccacg tagctcaatc ggttcacaag gagatgatca
     4261 ccaataagaa gggttggctc catggagctc gatggaatct taaaggcctg aagaaaaaaa
     4321 ctgcgtacaa taattgctat tatcaatgca atgataagag actcgccata ctctctccac
     4381 ttggtctttt gttttgcatg cctttctttc atttatcact cccgattgaa tcagctcatt
     4441 gctgatagtt cattgctcat agggtaaaga aaaagctgtt gctatgagct cagagctgcg
     4501 agctggttta ctcacattcg ttccggtgct gatacaccga gcagtgccaa tgcatctttg
     4561 atgacctgtt gcagtgttgc aagcagtaag agtctcgcca tagtcaattc ctggtctcca
     4621 acgataactc tcgttttatt ataataactg tgaaatctcc ccacaagatc gaggaggtaa
     4681 aaagttatcc tgtgcggttc aagacttttt gcgctaccct ctatcacatc aaagaaatgg
     4741 agtatgccct ttatcagggt tatctcatct ttctctgtca agaggtcgac tcttgcagtt
     4801 ttcaccgatg ccgggtcaat cccctcatcc tttgccaccc tgaaaatgct ttcaattctc
     4861 gcatgggcgt actggatata gaataccggg ttttcatttg tcgtcttctt tgcaaggtca
     4921 aggtcaaact cgagatgggc atcgctttta cgcataagaa aaaagaaccg cgccgcatcc
     4981 ctgcctacct cgttgagcac ttccttaagg gtagtaaatt gccctgcccg tgtagacatt
     5041 cccacaggct ttccatcctt gataagggtt acgaattgta ttaaaaggac tttcagtgag
     5101 tccttacccc tgccgagggc ctctatggat gctctcatac ggggaatgta tccatgatga
     5161 tcagaacccc atatatcgat gagagtatcg aaccttctgt caaatttgtc cttgtgatag
     5221 gctatgtcag aggcgaaata tgttttttca ccgtcagact tgatgagcac ccggtcttca
     5281 tccttttcaa acatacttgt cttaaaccag agtgctccgt ctttttcata ggcatatccc
     5341 ttatccttca acgtagcgag tgtctcttct acctcgccac tctcataaag cctcgtttcc
     5401 ctgaaataat tgtcgaaaag aacgccaaat tcctcgaggt cttttttgat gccatccaga
     5461 acaaagtcac tggcaaattt tgacatgaat gcaattgcct cagctttttc agccggaaca
     5521 gcaacgttgc tctcaacaat aagcgaggca atgtccttca cataatcacc ctgataataa
     5581 tgctcttcca gaggcacggc atcgcccgtt aactctttcc atcgtgcata ggttgactga
     5641 ccgagggtat caatctgctt accggcatca ttaacataat attcttttac cacgttataa
     5701 cctgccctct tcattatgtt tgcgaggaca tcaccaactg cagcccccct gccgtggcct
     5761 atatggagcg ggccggtcgg atttgagctt acaaattcaa tgagaacctt tcttccctca
     5821 ccaacatccg gaaccaatga atctaatccc tggtcagaga tttcccgcag tgccgcccgc
     5881 cacatgtcgt ctcttacata aaagttgata aagccttttg cagcaacctc cactttttcg
     5941 cagacaccat gaaactccat attcccgatc agtgtttttg caatgtcggt ggggcttttt
     6001 ctcacctttg gcgcgagcag gaaggcaata ttcgtggaat agtctccgtt ggcctcctct
     6061 cgcgggattt cgatgataat gttcacatcg atatcagggg gcaggaaccc tgattgcctg
     6121 caattctccc atgccttttt aacaatcgcg cttatttgct gctttaccat acgaattaac
     6181 tccctgtttg acagagcagt gtccggcccc tatggggtta cactgagaat ttctttataa
     6241 ttttccggtt ttcgttccct gaggaggggc cattctcgac ggatatgttg aaggtaatca
     6301 aaatctacat ctgccaaaag cacactatct gctatccctg ttggcccgcc tatcagttct
     6361 ccctcaggat ttacgcagaa gctcgctccg taaaaatcct gtttctcctc acttccggcc
     6421 ctgttgaccc tcattataaa gacgccattc gctattgcat tacctgatat ggcagtctgc
     6481 cagaggtgct gggatttaaa agcacaggct gtaggggcaa agataatatc cgcacctttc
     6541 aaggttaaga tccttgttcc ttccgggtac aggttgtccc atgaaatctg gatccctatt
     6601 ttgccgaagc ttgtttgaaa aaccggaaaa cctttgtcac ctgctgaaaa ataaaatttt
     6661 tcttcccaca gagatatatc aggtatgtga atttttcggt atacacctgc tacttcgcca
     6721 ttttcatcta ctacaacaca actgttataa aaccgggttt tgtctttctc aaaaatagga
     6781 agcagaatca ctatcccggc ggacttcgct ttttccctca tagctttgac cgtgttccca
     6841 tgaatgctct cactcagctc aaaagctttt tcatctctat ccttgggaaa ccaggggagg
     6901 ttaaacagct cctggaaaca tattatgctt gcaccatttg aaatagccgc atcaattgtc
     6961 cgaagggcat tttctacgtt cttatcctta tccactgaac atgcgaactg aattcctgct
     7021 attttcatcg tttattatct gttcgttgcg tggagtataa accaggggtc cagtctgtat
     7081 tttttcaaga cctcttcgat atggagattg caatggttcg aaaaagtatc gactgaaagg
     7141 cctgtcctcc agcccagctt catgaaaacc ggtgttaaaa gtttttccat gcttgagata
     7201 aaagacaatt ttgatgaaag atggttaatt attatgatct ttccaccttt cttcgtgacc
     7261 cttttcacct ctgacatgag ctgctccacg cttggtacca cgctggctac aaaaggcagg
     7321 acagcgtgat caaagctgtt gtcatcaaag cagatatttt ctccgtccat ctcaaagaga
     7381 tctacatggt taaaaccaaa cttctccttt ttggccttcg ctttttcaag cattttcctt
     7441 gtaatatcta tacccacaac tttgcaggat tgagggtaca gggaaaggga aagacctgtg
     7501 ccaacgccaa cctcaataat cctgtcgcca ttttttatcc caattttctc gaggccaagc
     7561 cttatcctgg gataaaaaaa ctgtgaaaag ataatatcgt aaaaacatga ataacgactg
     7621 tagatcttct gaatttctgc tttttgcaca aagagaaaag taactcaggc gcaggggtat
     7681 gtcaaggaaa aagaggactt gaacagacac tctctttggc aggggacatt taacccgggt
     7741 gcaagacaga tttaatttct gtatactttt gcagtgaagg tactccttat tcagccacca
     7801 attgaagatt actatgatac ctccatacgc acgtatcccc tgtctctgct ctatatcgcc
     7861 acaaaggtgc gggagatttg cgatgtctct atagcagatc tgaggagcaa cagaaaacca
     7921 aggcaaatca ttgaccatcc ttttcctgaa ctcgcacctt actatcgcaa agacaggtat
     7981 acacccatct ccctctttaa acagtactcc cgttttggtg cagacctggg tgagatcagg
     8041 cagagaattg tttttgaaaa accggatgtg gtcgctgtgt cttcgctctt cagcgcctat
     8101 tcaggagaag ctatggatat agcccggtgt gcgaaagaag tggatgacaa aatcattaca
     8161 gtggcaggag ggatccaccc tacccttttc cctgagcatg tgctgcagtc tccctttgtt
     8221 gactacgtgg tgagaggcga gggagaaaca cctttttcag tacttataga agctataaag
     8281 aaaggaacag tacaaagcat tgctggtact ggagggattg catcaaaaca tcatggcaga
     8341 tgcatcatac ctgatattca tatcgaagaa aacctcgatc ttgtaccgga tcgcaggctg
     8401 gttaatccct cggagtatcg gattggaaag aaaaaatata ccttcttcct cacatcccgc
     8461 ggatgcccat ttcattgtgc attctgtggc agacctcctt ttccctatcg cgcaagaggc
     8521 cttggcagtg tggaaaaaga gattgcagat tgcctttccc tggatatcca ggcagtcgat
     8581 tttgaagacg atatgcttac cctggaccgg cttcggtttc aaaacatact tgggctgttt
     8641 agaggtacag gactgactct ctcagcaatg aatggcattt actcggaaaa cctggatgtg
     8701 gccacccttg agcgcatgta cgaatccggt ttccgaaggc tcaacttttc ccttgttgat
     8761 gcatcagcat ccgtcatcaa catgcagaaa aggcataaca catgcaactt tgcaaaactc
     8821 ctcccttatc ttgaggcctc tcattttttg gtagagaccc atttcatcat cgggttacca
     8881 gaacaacggg ttgaacacat acttgacact atcattttcc tgatgggcaa gaagcttctt
     8941 ctcgggccca gcatcttcta ccttgcacca aatagtagtg tatacagccg cctcgcacca
     9001 gatcttcagg acacaaatca ttacttcaag cttatgcgtt caagcgcaat ggtccccttg
     9061 aatcccagta ttccgcggga tagcctttac acccttatga aactggtgcg gtttgttaat
     9121 cttgtaaaag gactgctcga tggaagtaat gtgcccacaa ggttatctga tatactcgaa
     9181 ctgcctgctg ttacgaaaaa tgatcgtgac agacaagtcc tcagcaccct gctcaatgaa
     9241 aaacaactca tctggttcga cagttcccac ggcactttcc tgcccgaacc gcacagcagc
     9301 gctattgtgc ggctgttctt ccatcgggcc aaaaacaaat ccatctgcgg ttttaaaacc
     9361 aggaaccggc tggttgtcga ttaatacgga catcgtgatt ggcatacgct tgctcagcac
     9421 tcttgttgtc ggggtattcc ttgtgtatgg tccacgcctt tcgtatagca caatacccat
     9481 catattttga ttgtaggctg catcttatat aatggtatga acttattacg aaagacactc
     9541 acactttgat agaacgtaaa ctggaataaa tttcccggga atcattcttt aaaattacct
     9601 cttgacaata ctgtccccat caatataatg acatatatca cacatggtag gatgatcagt
     9661 gaggggtagg caaaaggagg aacctatgcg cctgaccaca cttccctgga tcgtcaaact
     9721 gcatcgatta acgccccatc ttatccagaa gattacaatg cctgtcaaaa tgacatcgag
     9781 aaaagccagt ctcctcgtta cctgggccat gtgctggtat gtactggcaa ccgttatatt
     9841 gatagttctt catgcctgct cttgagatgg a
//
LOCUS       DAOOMC010000105         9719 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdus sp. isolate AMR_MDS_2685
            NODE_8115_length_9719_cov_5.020592, whole genome shotgun sequence.
ACCESSION   DAOOMC010000105 DAOOMC010000000
VERSION     DAOOMC010000105.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864760
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdus sp. (wastewater metagenome)
  ORGANISM  Syntrophorhabdus sp.
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae; Syntrophorhabdus.
REFERENCE   1  (bases 1 to 9719)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9719)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,729
            Genes (coding)                    :: 2,708
            CDSs (with protein)               :: 2,708
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 7 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9719
                     /organism="Syntrophorhabdus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8115_length_9719_cov_5.020592"
                     /isolate="AMR_MDS_2685"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2699756"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..233
                     /locus_tag="PK125_10445"
     CDS             <1..233
                     /locus_tag="PK125_10445"
                     /inference="COORDINATES: protein motif:HMM:NF012717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HPB38561.1"
                     /translation="PFGSAWVRYSSHYYIYALIFIAFDVDILYLFPAALSYTRGAKSY
                     EFWSLLVFMLILGLAILYAWGKGVFAWKRKVQ"
     gene            191..703
                     /locus_tag="PK125_10450"
     CDS             191..703
                     /locus_tag="PK125_10450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000236330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B family
                     protein"
                     /protein_id="HPB38562.1"
                     /translation="MGKGGFCLETQSAIIKFALLDKVLDLARANSLWPVTFGLACCAI
                     EMMSMSMARWDIARFGAEVFRPSPRQADLMLVTGTVTKKMAPSVVTLYEQMPSPKWVI
                     AIGNCAISGGPFLFDGQYNVVEGVDKLIPVDVYVPGCPPRPEGFLEGLMKLEEKITGK
                     RRFPMPESRW"
     gene            697..2271
                     /locus_tag="PK125_10455"
     CDS             697..2271
                     /locus_tag="PK125_10455"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF004739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit D"
                     /protein_id="HPB38563.1"
                     /translation="MVGKILEEIKASLVNVNFHDASYEQSGYHLSVDVQTGNIPDSVK
                     FFKDKAFYLEDLFCVDYIDYLELVYFFNRYEELCRVKVTSKVDAGKPIAPTISNSYTM
                     AHWYEREIHEFFGVYFDGHPNMTYLFLHEGIDHYPLRKKAVPVPDEDKKLLNSFKPQE
                     EEDCFFVNLGPQHPSTHGVLRVVVKMDGEYIISAEPVLGYLHRMHEKMAENRSYLQFL
                     PNPARIDYPGALLLNLAHVATVEKLCGIPVPERAQYIRTITCELNRIASHLLWLGAFP
                     ADLGALTPFMYAFDDREQILDILEDVTGSRLTYCYFRFGGLYNDVGDTFVDKTKAFIK
                     RMRERFPMYEKLITGNVIFVNRAKGTGIFQQVDAKKYGCTGPFVRSTGIPYDIRKAEP
                     YAVYDQVDFDIPVGTAGDNIDRYHVRVAEMEMSLRIIEQALAKLPSGPFKTEKVPKKL
                     KPPKGDVYYAVESSRGEAGVYVVSDESDTPYRMRWRVPSFSNLMTFPSLAEGNLIADA
                     IATLGSYDIVVPEIDR"
     gene            2325..3305
                     /gene="nuoH"
                     /locus_tag="PK125_10460"
     CDS             2325..3305
                     /gene="nuoH"
                     /locus_tag="PK125_10460"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF004741.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HPB38564.1"
                     /translation="MKTINNIIELVGWEAIKLLIGLIGVLVVVVVNALVLTWAERKVS
                     GHMQRRIGPKEVGPCGMLQPIADTIKLIAKELLTPQNVERPLYFLAPVIVFIPVMVSF
                     VVIPFDAYLQVKDINVGILVILAFSSLSVLSILLAGWGSNNKYSLIGSVRSVAQNIAY
                     EIPLLLALLAVVLMTNSLSLRDIVESQKGLWYIVSQPIAFLVFFIAGVAETNRTPFDL
                     PEAESELVAGFHTEYSGMRFALFFLAEYTNIFIICAITTTFFLGGYHGPILPGFVWFF
                     GKTYLLVFVILWFRWTFPRVRFDQLLNFSWKLLIPVSLLNLIVTGGILKL"
     gene            3322..3777
                     /locus_tag="PK125_10465"
     CDS             3322..3777
                     /locus_tag="PK125_10465"
                     /inference="COORDINATES: protein motif:HMM:NF024634.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HPB38565.1"
                     /translation="MALTNPVAIVKGAVSLIIGLGVTIKAFFSPVVTVQYPRVELKIS
                     PRFRGHTKLVADKDNPDKTSCIVCGMCEKNCPSGAIVKVVGEKKEGEKKKTLTSYILD
                     FTTCSQCGICVEICPVDALAFSAEYNRAVFTREGCHFDLVKEFEKRKIH"
     gene            3774..4283
                     /locus_tag="PK125_10470"
     CDS             3774..4283
                     /locus_tag="PK125_10470"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF012709.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HPB38566.1"
                     /translation="MINLGEPIFILIVIMTVAGALTTVLSGSVIYALIGLVATMFGIA
                     GLYVYLNAPFLAMMQILVYVGAITILIAFAIMLAGPFYKRPKEWTGIGKFSAALVVSV
                     ISFLTFFTIIMKVSWTEGKGAFNLLTKDIGRALFDRYAFPFELISLVIVVSIIGAIML
                     AMFSRGKND"
     gene            4276..4602
                     /gene="nuoK"
                     /locus_tag="PK125_10475"
     CDS             4276..4602
                     /gene="nuoK"
                     /locus_tag="PK125_10475"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF004320.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HPB38567.1"
                     /translation="MTDGFLSNSLNVYLFVGLFLLCCGILGVLYRRTLIAMLISIELI
                     MNGAGLNFVAFNRFVSPEGPEGMIFTLFVMGIAAAEAAIALAIIILIFRKYRSIESAE
                     MEEMKD"
     gene            4605..6077
                     /locus_tag="PK125_10480"
     CDS             4605..6077
                     /locus_tag="PK125_10480"
                     /inference="COORDINATES: protein motif:HMM:NF006236.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monovalent cation/H+ antiporter subunit D family
                     protein"
                     /protein_id="HPB38568.1"
                     /translation="MTVESALPLVPIIIVIITTILIFLSGKRPNLREFWSVAGSILTF
                     ISVAIMVPMIFNNQKIVYTLSTIAPGISLNFRVDALSLIFGIVSSFLWIFATFYNIGY
                     MRSLDEHAQTRYYTCFAVAILGAQGVSYSGSLFSLYLFYEIITIFTYPLVAHHQDEEG
                     YAGAKKYIVYLMGTSKGLLLPATVLTYVFTGTLDFADNITSGIFTKGTDGVWITVTYG
                     LFIFGFAKAAIMPLHNWLPSAMVAPTPVSALLHAVAVVKAGVFCISRVMLSVFGLELL
                     QALNLGLCTAYLVSFTILVASIIALTKDDLKARLAYSTVSQLSYIVLGVALLDPTGVL
                     GGIIHIVNHGFSKITLFFCAGAIYVATRKKKISDMAGIGYAMPFTMGAFAIASLSMIG
                     VPPVAGFVSKWYLLNGAMSIHNLPIVLVLMASTLLNAGYFAPITIKAFFQGRKEKWTA
                     ADIKEAPVTMVVPIVLAALISVVLGIFPQFFSQLIGRLMP"
     gene            6166..6414
                     /locus_tag="PK125_10485"
     CDS             6166..6414
                     /locus_tag="PK125_10485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB38569.1"
                     /translation="MRIVQYIEFLRRNMKGLVRALIVYLIVLVVFDVTLSRHDAHYII
                     DKVYAFWTIFGIVGCFILIKVAKGIAHLFLGKDEDFYG"
     gene            6407..8167
                     /locus_tag="PK125_10490"
     CDS             6407..8167
                     /locus_tag="PK125_10490"
                     /inference="COORDINATES: protein motif:HMM:NF009310.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)/H(+) antiporter subunit D"
                     /protein_id="HPB38570.1"
                     /translation="MDRWIHPAIFFFIGALILPLFRGKAKKFVILLIPALSIIDVAFM
                     MEGIYGSYNFLNADIIFGRVDKLSLVFAWVFTIMGFLGAIYALHVEEDGHHMAAYFYV
                     GASLGAIFAGDYLTLFIFWEIMAFSSVFLVWYRKEKKSITAGYRYLLVHVFGGLCFFT
                     GMMLYYYKTGNMVFDKILPANAGVAEYLILAGFALNAAVIPLHAWLPDAYPEATVAGA
                     VFMCAFTTKTAVYVLARGFPGFEILAILGTAMTVYGVFYAVIENDMRRVLAYHIVSQV
                     GYMVAGVGIGTEMALNGACAHAFAHILYKALLFMGAGSVLYMVGTAKLSKLGGLYKYM
                     PLTMIFYVVGAISISGFPLFSGFVSKSMIVASAHHEGRIWLMLLMNLAGIGTFLSVGL
                     KVTYFAFFGKESAVQPKDPPKNMLWSMGLTSALCFIIGVYPNSLYVLLPFPVEYHPYN
                     LHHLSEMLQILSFTGLVFFLLVKKLAPEDKINLDLDIFYRAGAWVFMKIDEKIISVLD
                     SLWGEAYRAIGLSLLFKNARLSYGFDRKVIDGVVDGSATAVRMVGFVVRKLQTGKVQA
                     YIGLALLMFFLIVWILLVRM"
     gene            8180..9664
                     /locus_tag="PK125_10495"
     CDS             8180..9664
                     /locus_tag="PK125_10495"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HPB38571.1"
                     /translation="MSDYLIMNPLNFPILTTTIFIPLAGAILLLLMRSDKIVKGIAFT
                     TGIINFLISLLLLVYFDSSTHLFQFGEFARWIPTYNINYILGIDGITIFLILLTTILA
                     PMCVLCSWTAIESRVKEFMFCILVMESAMVGVFCALDLILFYIFWEAMLIPMYLLIAV
                     WGGPNKDYASVKFFIYTLFGSVFLLVAIIGIYLATGSFNIPNAMFRDYSFNFQFWVFL
                     AFAIAFAIKVPMFPFHTWLPAAHTEAPTAGSVFLASVLLKMGTYGFLRFCLPITPKAS
                     VFFAPLMLVLSVIAIIYGGFVCLSQTDMKKLIAYSSVAHMGFATLGIFSLTLFGFQGA
                     LLQMLNHGVTTGALFLCVGIVYERTHSREIYDNAGLGKIMPIYVGFFGLFSISSFAFP
                     GTNNFVGELYILIGTFANHKAAGFFAVIGAILAAAYMLKLLKQIIWGREDKRSIKDMN
                     VREIAYLIPLAVFVLWMGLFPAPFVHTFERTITHLTSRLQVYFP"
BASE COUNT         2386 a         2066 c         2233 g         3034 t
ORIGIN      
        1 aacctttcgg cagcgcgtgg gttagataca gctcccacta ttatatttat gcacttattt
       61 ttatagcctt tgatgttgac attctgtacc tctttccagc tgccttaagc tacacaaggg
      121 gtgcaaaatc atatgagttc tggtcactgc tcgtgtttat gctcatcctg ggccttgcta
      181 ttctgtacgc ctggggaaag ggggtttttg cctggaaacg caaagtgcaa taataaaatt
      241 tgcccttctt gacaaggtac tcgatctggc acgagcaaac tccctgtggc ctgtgacctt
      301 tggtctcgca tgctgtgcca tcgaaatgat gtctatgtcc atggcccgct gggatattgc
      361 gcgcttcgga gcagaagtct tccggcccag cccacggcaa gcagacctga tgttggttac
      421 tggaacagta accaagaaaa tggcgccatc tgtggttact ctttacgaac agatgccttc
      481 accaaagtgg gtgattgcga tcggtaattg cgccatatca ggtggtcctt tcctgttcga
      541 tggtcaatac aacgttgttg agggtgtcga taagctgatt ccagttgatg tgtatgtacc
      601 tggttgccct ccaagaccgg aagggttcct tgaaggcctt atgaaacttg aagaaaaaat
      661 aacagggaaa cgtcgtttcc cgatgccgga aagcagatgg taggaaagat tctcgaagag
      721 atcaaagcat cattggtgaa tgtaaacttc catgacgcct cttacgagca aagtggctat
      781 catctatctg tagatgtaca gaccgggaac atcccggata gtgttaaatt cttcaaggat
      841 aaggcattct accttgaaga cctgttttgt gttgattaca ttgactatct ggaactggtc
      901 tattttttta atagatacga agagttgtgt agggtgaagg ttaccagcaa ggttgatgca
      961 ggtaaaccaa tagcgccaac gatctccaac agttatacaa tggcacactg gtatgaacgg
     1021 gagatacacg aattttttgg tgtttatttt gatggtcatc ccaatatgac atatcttttt
     1081 ctccacgagg gaatagacca ctatcctctc cggaagaaag cggtgcctgt acctgatgaa
     1141 gataagaagc ttctgaattc tttcaaaccc caggaagagg aagactgttt ctttgtgaat
     1201 cttggtcccc agcatccgag cacccatgga gtactcaggg ttgtagtaaa aatggatggt
     1261 gaatatatca taagcgcaga accggtgctg ggttatcttc acagaatgca cgaaaagatg
     1321 gctgaaaaca ggagctatct ccaatttctt cccaatcctg cccggatcga ttaccctggg
     1381 gctctgcttc ttaacctggc acatgtggcc acagtagaaa agctctgcgg tatacctgtt
     1441 cctgagcggg cgcagtatat aagaaccatt acatgtgagc tgaacaggat tgcaagccac
     1501 ctactctggc ttggtgcttt tccggcggac ctcggtgcgt taaccccttt catgtatgca
     1561 ttcgatgaca gagaacaaat ccttgacata ttggaagatg taacaggttc gcgactcaca
     1621 tattgttatt ttcgctttgg tggcctttac aacgatgttg gcgacacttt tgtcgacaag
     1681 acgaaagctt tcatcaaacg catgcgtgaa cgattcccca tgtatgaaaa gctgattact
     1741 ggtaacgtaa tatttgtgaa tagagcgaaa ggtacaggta ttttccagca ggtagatgca
     1801 aaaaagtatg gctgtaccgg tccgtttgtg aggagcaccg gcattcccta tgatatacgt
     1861 aaggcggagc cttatgctgt ctatgatcag gttgatttcg acatacccgt tggtacggca
     1921 ggagacaaca tagatcgata tcacgtgcga gtagctgaaa tggagatgag ccttcggatt
     1981 atagaacagg ctctcgcaaa actcccctca gggccgttta agactgaaaa agtcccgaaa
     2041 aaactgaaac cgcctaaagg agatgtttat tatgcagttg agtcatcacg gggcgaggct
     2101 ggagtatacg ttgtgagtga tgagagtgac actccttaca ggatgagatg gagggttcct
     2161 tccttttcaa acctcatgac attcccatct ctcgctgaag gcaatctcat tgctgatgcc
     2221 atagcaaccc ttggaagtta tgatattgtt gtgccggaga tagatagata gcggcggcag
     2281 aaactgaggt cgagatggga gaaagaagta aaagacaata agtgatgaaa acgatcaata
     2341 atataataga gttagttgga tgggaagcta ttaagctgtt aatagggctc atcggcgttc
     2401 ttgttgtggt tgtggtcaat gctcttgtgt tgacgtgggc agagcgtaaa gtcagtggcc
     2461 atatgcagag aaggataggg ccaaaagagg ttggtccttg cggtatgctc caacccatcg
     2521 ctgacacaat taagcttata gcgaaagagc tcctcacccc acagaacgtg gaacggccac
     2581 tctattttct cgcaccggtg atcgtcttta tacctgttat ggtctctttt gttgtaattc
     2641 ctttcgatgc atatcttcag gtaaaagaca ttaatgttgg aatcctcgtt attctcgctt
     2701 tctcatcgtt gtctgtactt tccatccttc ttgcgggctg gggctccaac aataaatatt
     2761 cacttatcgg gtcggtgcgg agcgttgctc agaacatcgc ttacgaaata ccactactgc
     2821 ttgccttact cgctgttgtg ctcatgacaa attcactttc tctcagagat atagtggagt
     2881 cacagaaagg gctgtggtat attgtctcgc agccaatcgc ctttcttgta ttttttatcg
     2941 caggagtcgc agaaacaaac cgaacaccct ttgacctacc tgaggcagaa agcgagctcg
     3001 ttgcagggtt ccatacagaa tattctggta tgcgttttgc cctctttttt ctcgcagaat
     3061 atacaaacat tttcatcatt tgcgcgatta caaccacctt ctttcttggt gggtatcatg
     3121 gacctattct gcccggcttc gtgtggtttt ttggaaagac ctaccttctt gtttttgtga
     3181 ttttgtggtt ccggtggacc tttccgagag tcaggttcga tcagcttctc aacttttcat
     3241 ggaaactgct catacctgtc tccttattaa accttatcgt tacagggggg attctaaagt
     3301 tatgacggac ggtggtatag aatggccttg accaatcctg tggcaatagt aaaaggcgct
     3361 gtatccctca ttatagggct tggtgtaaca attaaggcat tttttagccc tgttgttaca
     3421 gttcaatacc ccagggtaga actaaaaata tcaccacgat tcagaggtca cacaaaactt
     3481 gtggctgaca aggataatcc tgacaaaaca tcatgtattg tatgcggcat gtgtgaaaaa
     3541 aattgccctt caggagcaat tgtcaaggta gtgggtgaaa agaaggaagg agaaaagaaa
     3601 aagacattaa catcctacat ccttgacttc accacgtgta gccaatgcgg gatttgtgtg
     3661 gagatatgtc cggttgacgc ccttgccttc tcggcagaat ataaccgtgc agtttttacc
     3721 agggaaggtt gtcactttga tctagttaaa gagttcgaaa agaggaaaat acattgataa
     3781 acctcggtga gcctatattc atactcatag tgatcatgac agtggctggt gctcttacaa
     3841 ctgttctgtc gggatctgtc atttatgcgt tgatcggcct ggtagccacc atgttcggta
     3901 tagctggcct gtatgtgtac ctcaatgcac catttcttgc tatgatgcaa attcttgtat
     3961 atgttggtgc cataacaatc ctgattgcat ttgctataat gcttgctgga cctttttaca
     4021 aaagaccgaa ggaatggact ggaataggca aatttagtgc agcactcgtc gtctcagtca
     4081 tctctttttt aacattcttc accatcatca tgaaggtttc ttggactgaa ggaaaaggag
     4141 cgttcaacct gttgaccaaa gatataggtc gtgccctttt tgaccgatat gcttttcctt
     4201 ttgaattaat ttctcttgtt attgtggtaa gcattattgg cgccattatg ctggcaatgt
     4261 tctcaagggg gaaaaatgac tgacggtttt ctctccaata gtctgaacgt ctacctcttt
     4321 gtggggctct ttctattgtg ttgcgggatc ttgggcgttc tttaccgaag aactttgatt
     4381 gccatgttga tttccatcga actcataatg aacggagcag ggctcaattt cgttgctttc
     4441 aaccgatttg tctcgcctga agggcctgaa ggaatgatct ttaccctttt tgtcatggga
     4501 attgcagcag cagaggcagc cattgcgctt gctattatca tattgatttt cagaaaatac
     4561 aggagtatcg aaagcgcaga gatggaggag atgaaagact gacaatgacc gttgaaagtg
     4621 cactccctct tgtcccgata attattgtta ttatcaccac tattcttatt tttctttcag
     4681 gtaagagacc aaatcttaga gagttttggt ctgttgcagg gtctatactt acgtttattt
     4741 ctgtagcaat catggttccc atgattttta ataatcaaaa aatcgtatac actttgtcaa
     4801 ctatagctcc cgggatctcc ctgaatttca gggttgatgc cctttcactt atattcggga
     4861 ttgtatcgtc gtttctgtgg atttttgcaa ccttctacaa catcggttat atgcgttccc
     4921 ttgatgagca tgcccagacc agatattaca cgtgttttgc agtggcgatt cttggagccc
     4981 agggcgtctc atattcagga agtcttttct ccttgtatct gttttatgaa attataacga
     5041 tttttaccta cccccttgtt gcacatcatc aggatgaaga ggggtacgca ggagccaaaa
     5101 agtatattgt ttaccttatg gggacatcaa agggattgtt gcttcctgca acagttctta
     5161 cctatgtctt taccggaacc cttgattttg ctgataacat tacctccggc atcttcacca
     5221 aaggtactga tggggtttgg attaccgtga catacggcct gttcatcttc ggatttgcca
     5281 aagcagcaat catgccactc cataattggc tgccttcagc aatggttgcg cctacccctg
     5341 tcagtgcact tctccatgcc gttgcagttg tgaaagcagg tgtattctgc atttctcgag
     5401 tgatgctatc agtgtttggc ctcgaacttc tccaggccct taacctcggc ctttgtactg
     5461 cctatctcgt atccttcacc attcttgttg catcaattat tgccctaaca aaagatgatc
     5521 tcaaggctcg gcttgcatac tccaccgtga gccagctttc ttatattgtt cttggcgtag
     5581 cactcctcga tccgacaggt gtgcttggcg gcattatcca tattgtgaac catgggttta
     5641 gtaagataac acttttcttc tgtgccggtg caatctatgt ggcaacccgc aaaaagaaga
     5701 ttagtgatat ggcagggata ggttatgcaa tgcctttcac tatgggcgct tttgctatag
     5761 cgtcgttgag catgataggt gtaccacccg tggcaggatt tgtttcgaaa tggtacctcc
     5821 tgaatggagc aatgtccatc cataacttac ccatagttct cgttcttatg gcaagcacat
     5881 tactgaacgc gggttatttt gctcccatca cgataaaagc ttttttccag ggaagaaaag
     5941 aaaaatggac tgccgcagat atcaaggagg ctccggtaac tatggttgtt ccaattgttc
     6001 ttgcagcctt gatatcagta gtactgggta tctttcctca attcttttca cagcttatcg
     6061 ggaggttgat gccgtgatga atccgacgaa tattgcgtgt tatgccttac aggtgagtgg
     6121 taaaccggga aaatcaagaa gtatatcgag caggaggcag atcctgtgag gatagttcag
     6181 tatatagagt tccttagacg aaatatgaag ggtctggtta gagccttgat tgtgtatctt
     6241 attgtcctcg ttgtttttga tgtgaccctg tcgaggcatg atgcacatta tataattgat
     6301 aaggtttacg ctttctggac catctttggt atagtcggat gttttatcct tatcaaagta
     6361 gcaaaaggta ttgcccatct ttttttggga aaggacgagg atttttatgg ataggtggat
     6421 tcatccggca attttctttt tcatcggggc tttaatcctc ccccttttca ggggcaaggc
     6481 gaagaagttt gtaattctgt taatccccgc tctctccatt atcgatgttg cctttatgat
     6541 ggagggtata tacggaagtt acaattttct caatgctgat atcatttttg gcagggtaga
     6601 caagctgagt cttgtttttg cctgggtttt tactattatg ggcttccttg gggcaatcta
     6661 tgctctccat gttgaagagg acggccatca catggcagct tacttctatg ttggagcttc
     6721 gctcggagca atttttgcag gagattacct gaccctcttt attttctggg agataatggc
     6781 cttttcctcc gtctttcttg tgtggtacag gaaggaaaag aaatccataa ccgcaggata
     6841 taggtacctc cttgtccatg tatttggtgg tctttgtttc ttcaccggta tgatgctcta
     6901 ctattacaag acagggaata tggtttttga caaaatattg ccggccaatg caggtgttgc
     6961 cgagtacctg atccttgcag ggtttgcctt gaatgcagcc gttatcccgt tgcacgcatg
     7021 gctgccagat gcataccctg aagcgacggt agcgggtgct gtctttatgt gtgcctttac
     7081 taccaagaca gctgtgtatg tgttagcaag aggattcccg ggttttgaaa tacttgcaat
     7141 cttgggtaca gccatgacag tgtacggtgt tttttatgct gttatagaaa atgatatgcg
     7201 tcgcgtcctt gcctatcata ttgtcagcca ggtgggatac atggttgccg gtgttggaat
     7261 agggacagaa atggcgctca atggggcttg cgcccacgct tttgcccaca tcctctataa
     7321 agcactgctt ttcatgggtg ccggttcagt cctctatatg gtaggaacgg caaaactttc
     7381 aaaactcggt ggtctttata agtatatgcc cctaacaatg atcttttacg tagtgggggc
     7441 aatttcaatc tcaggatttc cgctattcag tggttttgtg agtaaatcca tgattgttgc
     7501 atctgcccac catgagggga gaatctggct catgttgctc atgaaccttg ccggaatagg
     7561 caccttcctg tcagttggcc tgaaggtcac ctattttgcc tttttcggaa aggaatcagc
     7621 cgtacaaccg aaggaccccc caaaaaacat gctctggtca atgggcctca cctcagccct
     7681 ttgtttcatt attggggtat atccaaactc attatatgtg cttctcccgt tccctgttga
     7741 ataccatccg tacaacctcc atcatctttc tgagatgctg caaattcttt cgtttacagg
     7801 cctcgtgttc tttctccttg taaagaagct tgcccctgaa gacaagataa atcttgatct
     7861 ggatattttt tacagagccg gcgcatgggt gtttatgaag attgatgaaa aaattatcag
     7921 tgtgcttgat agcctctggg gggaagctta tagagcaata ggtctttcac tgcttttcaa
     7981 aaatgctcga ttgtcatatg gatttgatag aaaggttata gatggcgttg ttgacggatc
     8041 tgcaacagca gttagaatgg tgggttttgt agtgcgtaaa cttcaaacag gtaaagttca
     8101 ggcctatata gggcttgcgc tgttaatgtt ttttctcatt gtgtggattc ttttggtaag
     8161 gatgtaaagg tgaactgcta tgagtgacta tttgataatg aatcctctta acttcccaat
     8221 ccttaccacg acaatcttta ttcctttggc tggagctata ctcctccttc ttatgaggag
     8281 cgataagatt gtgaagggta tcgccttcac gacaggaatc attaattttc tcatttcact
     8341 tctgcttctt gtatattttg attcttcaac ccatctgttc caatttggtg aatttgcccg
     8401 atggatacct acttacaata tcaactatat tctcgggatc gatggaataa ccatcttcct
     8461 catcctcctt actactatcc tggcgccaat gtgtgttctc tgttcatgga ctgctattga
     8521 gagcagggtg aaagagttca tgttctgcat ccttgttatg gaaagcgcca tggttggtgt
     8581 tttctgtgcc cttgacctca ttctctttta tatattctgg gaggccatgc ttatcccaat
     8641 gtacctactc atagcggtgt ggggtggccc aaacaaggac tatgcatcgg tgaaattttt
     8701 tatttatacg ctatttggaa gtgttttcct tctagttgct atcattggca tctatctggc
     8761 tacagggagc tttaacatac cgaatgccat gttcagggat tacagcttca attttcagtt
     8821 ctgggttttt ctcgcttttg ccattgcctt tgccattaag gtccccatgt tcccttttca
     8881 tacatggttg cccgctgctc acacagaagc accaactgca ggcagtgttt tccttgccag
     8941 tgtgcttctg aagatgggaa cctacgggtt cttgcgtttt tgcctaccta taacaccgaa
     9001 agcgagtgtc ttttttgccc ccttgatgct tgttttgtca gtaatcgcaa ttatatatgg
     9061 tggttttgtc tgtctgagcc agaccgacat gaaaaagttg atcgcctatt cgagtgttgc
     9121 ccacatgggt tttgctacgt tgggtatatt ctcactcaca ctatttggct tccagggtgc
     9181 ccttcttcag atgttaaatc acggcgtcac aaccggcgca ctgtttcttt gcgttggaat
     9241 tgtttatgag aggacccata gcagggagat atatgacaat gctggccttg gaaaaatcat
     9301 gcctatctat gtaggatttt ttggcctttt ttctatctcc tcctttgcat ttccaggaac
     9361 gaataatttt gtgggcgagc tttatatcct tatcggcacc tttgcaaacc acaaagcagc
     9421 aggatttttt gctgtgatag gagcaattct ggctgcagcg tatatgctga aactcttaaa
     9481 acagataatc tggggaaggg aagacaagag gtcaattaaa gacatgaatg taagggagat
     9541 tgcatacctt attcctcttg cggtttttgt tctatggatg ggcctttttc cagcgccttt
     9601 tgtacatacc tttgaaagga ctattaccca tctgacttca cgattacagg tctatttccc
     9661 atgagaggat cgattatgac catactgcct gaactctaca tgctcttttt ttcaattgt
//
LOCUS       DAOOMC010000106         9706 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdus sp. isolate AMR_MDS_2685
            NODE_8135_length_9706_cov_6.092840, whole genome shotgun sequence.
ACCESSION   DAOOMC010000106 DAOOMC010000000
VERSION     DAOOMC010000106.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864760
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdus sp. (wastewater metagenome)
  ORGANISM  Syntrophorhabdus sp.
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae; Syntrophorhabdus.
REFERENCE   1  (bases 1 to 9706)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9706)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,729
            Genes (coding)                    :: 2,708
            CDSs (with protein)               :: 2,708
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 7 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9706
                     /organism="Syntrophorhabdus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8135_length_9706_cov_6.092840"
                     /isolate="AMR_MDS_2685"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2699756"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            501..1670
                     /gene="dsrA"
                     /locus_tag="PK125_10500"
     CDS             501..1670
                     /gene="dsrA"
                     /locus_tag="PK125_10500"
                     /EC_number="1.8.99.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR02064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018551 - hydrogensulfite reductase
                     activity [Evidence IEA];
                     GO_process: GO:0019420 - dissimilatory sulfate reduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dissimilatory-type sulfite reductase subunit
                     alpha"
                     /protein_id="HPB38572.1"
                     /translation="MADETPLLDELEKGPWPSFVTEIKKAGEKNKAAKDLLGVLEKSY
                     KDRITHWKHGGIVGVTGYGSGVIGRYCDSPEEFPNAAMFHTVRVNQPAGWYYKTDSLR
                     KICDIWEKYGSGLTNMHGSTGDIILLGTTTDNLQPCFDALTEAGFDLGGSGSALRTLS
                     ACVGPSRCEWANIDTLGLIRDLTMEFQDEIHRPRWPYKFKIKASGCPNDCVASIARAD
                     FTIIGTWKDSIRIDQAEVQNYVRNGFNVEKHVVSRCPTEALKLDPQTGELKVKSEDCV
                     RCMHCINLMTRAIRPGIEKGATILIGGKAPIIKGAYLSWVLVPFMKLEPPYDDLKDLL
                     RKIWDWWDENGRTRERVAELIERLGLKTFLREIGLKPVPQMVKYPRSNPYVFFDK"
     gene            1691..2755
                     /gene="dsrB"
                     /locus_tag="PK125_10505"
     CDS             1691..2755
                     /gene="dsrB"
                     /locus_tag="PK125_10505"
                     /EC_number="1.8.99.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR02066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018551 - hydrogensulfite reductase
                     activity [Evidence IEA];
                     GO_process: GO:0019420 - dissimilatory sulfate reduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dissimilatory-type sulfite reductase subunit
                     beta"
                     /protein_id="HPB38573.1"
                     /translation="MGKTDIGPPDFWDMLPEAIKRNYGTWKYHEHVRPGVLKHVSESG
                     EQLYTIRAGSTRLLSIDFIRNICDIADEFCDGHLRFTSRSNIEFMTTDESKVEQIIGA
                     LNAMGLPVGGTGNSISNIVHTQGWIHCHSAATDASGLVKAIMDGVYDHFTDMKLPNHL
                     RIAVACCINMCGAVHCSDIAVVGVHTKPPKPDHERVIAQCEIPTTIQSCPTGAIRRHP
                     DATIKSVVVREDLCMYCGNCYTVCPAMPLADAEGDGVAIYAGGKVANARKPPMFSRMV
                     VPFLPNKPPRWQEVVDIIIKIIDVYSNDAKPAERLGEWVERIGWEAFFRKTSIPFTEQ
                     HIDDFSHAVETFRSTTMFKW"
     gene            2771..2980
                     /locus_tag="PK125_10510"
     CDS             2771..2980
                     /locus_tag="PK125_10510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB38574.1"
                     /translation="MPLADEISNELKNKIVEYLKTVSKAKNKEVAKAIDTDKHLVDKA
                     IAELSKEGKIEYIYLGASFIKLKDT"
     gene            3006..4436
                     /gene="cobB"
                     /locus_tag="PK125_10515"
     CDS             3006..4436
                     /gene="cobB"
                     /locus_tag="PK125_10515"
                     /EC_number="6.3.5.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0042242 - cobyrinic acid a,c-diamide
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenobyrinic acid a,c-diamide synthase
                     (glutamine-hydrolyzing)"
                     /protein_id="HPB38575.1"
                     /translation="MQDKQNVTPRIMIGAPNGRSGKTMVSVGLCALLHKNGLSVQPFK
                     KGPDYIDASWLTAASGKACRNLDAYLMSKDVIISNFCKSSLGNDFALIEGNMGLYDGF
                     DGGEEGSSAHLARVLKTPVVLVVNTARMTRSIAALIKGYMDFEAGTNVTGVILNNVAG
                     PRHEKKLSDAVERYCKIPVLGCIPRNQALAIHERHLGLVPFQEASSGTIGIERILRTI
                     EKHLDIESLISIAKSAQGLPISHSTTQQRPKVSVRIGVMMDHAFHFYYPENLEALEQE
                     GAELVLIDAMHDDKLEAIDGLYIGGGFPELYMSKLQLNETLRVQIATSIEDGLPVYAE
                     CGGLIYLCDRIVIDGVPYTGVGAIPSSVEFFTKPQAHGYSKLEVKYENPFYPVGMQIY
                     GHEFHYSRLTCAKGLHCALELTRGQGIDGKMDGIVYRNVFASYTHIHASGTSSWAKSF
                     VSVASRNKGIGKSAFEQESPREVGHG"
     gene            4429..4629
                     /locus_tag="PK125_10520"
     CDS             4429..4629
                     /locus_tag="PK125_10520"
                     /inference="COORDINATES: protein motif:HMM:NF016811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YHS domain-containing protein"
                     /protein_id="HPB38576.1"
                     /translation="MAEGTETVKCPVCGTEIEVGSQVAKLYGAVRAAPRATSSHEGKE
                     YHFCCKACKKKFDTEPTHYIKK"
     gene            4640..4957
                     /locus_tag="PK125_10525"
     CDS             4640..4957
                     /locus_tag="PK125_10525"
                     /inference="COORDINATES: protein motif:HMM:TIGR03342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TusE/DsrC/DsvC family sulfur relay protein"
                     /protein_id="HPB38577.1"
                     /translation="MAKTTLGGIEIEVDEDGFIQEPEKWNKEVAEDLSKLEQAFPMGE
                     DHWKIVNYLRDYYLKYEIAPPIRMLVKQTGIDLKTIYQLFPGGPAKGACKVAGLPKPT
                     GCV"
     gene            5037..5942
                     /locus_tag="PK125_10530"
     CDS             5037..5942
                     /locus_tag="PK125_10530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB38578.1"
                     /translation="MDIFLILLTCFAYIFILVAYSAKIVKYARMPIHLRWELFTATKT
                     EKKPYHHGLTAFEAQPFQSSRWRLSNVWSFLKEYLWFHSYLSHDKTYWCFLYIAHIGF
                     VSLIIFQAVCIATALGTFLAHDQTITAERATGSLVALRILLAIISFAAGIIGNIGLMV
                     YRISHRDLRLYTPPLTFIGYCISICTCLIGFILLLTNDPHFSGYVLFFVGMFQIKPIA
                     VSGLLGLFIFLNILHLIYLPFTPGFHYVTRFFAFFNVLWDTRPNQKGSPVEKAIGQVM
                     GKKITWSAPHIKSGQTWEQAAKHRS"
     gene            6039..7634
                     /locus_tag="PK125_10535"
     CDS             6039..7634
                     /locus_tag="PK125_10535"
                     /inference="COORDINATES: protein motif:HMM:NF014774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HPB38579.1"
                     /translation="MRIEDISKKSDQIIKIRQSDLLPLPYPYNNPEKDTAMRELSSDQ
                     RERYEASLDGVIGVGIQRPSSKAEEELFIHKFLSGLAKLFEKENNWTFLHPLYHSLEY
                     CVKCQICNDSCPAYIASGRKEIYRPTYRPEVLRRIVDKYIKKKNRASLKLTGSDIELN
                     WTLIARLGELAYRCTLCRKCTQACPLGIDNGLISHEIRKVFSQEMGIAPKELHEQGTV
                     QQLKVGSSTGINPRALENIIEFMENDIEEKIGRKIKIPVDKKGAEILLLHNAGEFMSW
                     PENTEAFAIIFDAAGIDWTLSSETVGYDAVNYGVWYDDIQLARVATRQAEIARNLGVK
                     KINVGECGHAHKAMIVVADRVLTGDLNIPRESALPILRDIVVGDKIKLDSSRNNFPIT
                     LHDPCNMVRLMGIVEPQREILKKICPQFLEMEPHGVDNYCCGGGSGFAIMQSMNFPDW
                     RSAVSGRIKLKQILEVFQDTISPDVKKYVCAPCSNCKGQIRDLLNYYNVWDECGIFYG
                     GLAELIVNAMIDLEKPFIEWEWH"
     gene            7747..8082
                     /locus_tag="PK125_10540"
     CDS             7747..8082
                     /locus_tag="PK125_10540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB38580.1"
                     /translation="MKGKVILLNSEYIGREDETLGFEILMTLLNSLLKRNDKPEAIIL
                     WNTGVKLMTSQSPASALLRELELAGVKILGGVLCLNDLCIADAVSVGQAVTMDDILDL
                     ILHNEVISL"
     gene            8132..8230
                     /locus_tag="PK125_10545"
     CDS             8132..8230
                     /locus_tag="PK125_10545"
                     /inference="COORDINATES: protein motif:HMM:TIGR04560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein THX"
                     /protein_id="HPB38581.1"
                     /translation="MGKGDQRTKRGKIARGTHGKTRPKKKGKKVSK"
     gene            8350..8760
                     /locus_tag="PK125_10550"
     CDS             8350..8760
                     /locus_tag="PK125_10550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB38582.1"
                     /translation="MGILQDIERLGLKLDDDALEHIKMIQEDIVRMNQDELVRNTLIE
                     MHISEFIEMARPDIMTALLDDKNREVEQLTKEFQRKFDSQGNRICEEHTCDSIIEVHR
                     CTYCGKYVCKEHNYGSEDLRCCYDCSIARTYGKK"
BASE COUNT         2868 a         2047 c         2265 g         2526 t
ORIGIN      
        1 gccagttttt tctgtaattc cttaatagtc atgtagacct cctcttctaa tatttatttc
       61 tctctatcct gttccacact tattcagtaa atataattcg tctttaatca tcacgtctat
      121 gacaatattt tttcttgtgc accccttaat tatggcgtta aattcagtct gactcgaatt
      181 ttgccaattg agaaatcaca catattccct gaaacgccag tattgtatat tgtggctgct
      241 attccctggc caatccggca tttttccacc tttgctttta acaagagttc attgacaagc
      301 tgacttttcg ctgctataaa gaatttatta ttttaaaaaa cattgagata aagtatttta
      361 ttgtgactat acaaatagat tttcatgata aattttgttt gttttgttta gctcccttcc
      421 gtttatgtgc cctttccaca gcattaatgg ggtgcacttc gcataaatat aagtaaaaat
      481 aagcactgga ggtgaaccat atggcagatg aaaccccttt attagatgaa ctcgaaaaag
      541 ggccatggcc gagttttgtc actgagatca agaaagcagg ggaaaaaaac aaggcagcca
      601 aggatctctt gggggttctg gaaaaatcct ataaggatag gattactcat tggaaacatg
      661 gtgggattgt aggtgtcaca ggttacggtt caggcgtgat cggtcgatat tgcgactccc
      721 ctgaagaatt cccaaacgct gctatgttcc atacagtgcg ggtaaaccag ccagctggat
      781 ggtattacaa gaccgattca ttacggaaga tctgcgatat ttgggaaaaa tatggaagtg
      841 gccttacaaa tatgcatggt tctacagggg atattatact ccttggtacc acaacagaca
      901 atctacaacc atgtttcgat gcgcttacag aagcagggtt tgatctgggt ggttcgggtt
      961 ctgcattgcg aaccctttct gcatgcgttg gcccatcccg gtgtgaatgg gcaaatatcg
     1021 acaccctggg ccttatccgt gacttaacaa tggaattcca ggatgagata catcgaccca
     1081 gatggcctta caaatttaaa atcaaggcat caggatgccc caacgactgt gttgcttcca
     1141 ttgccagggc agattttact atcataggca cctggaaaga ctcaatcagg atagaccagg
     1201 cagaagtaca gaactacgtg aggaatggtt ttaatgttga aaaacatgtt gtcagcaggt
     1261 gccctaccga agctctcaag ttagaccctc aaacagggga gctcaaggtt aagtcggaag
     1321 attgcgttcg ctgtatgcac tgtataaatt tgatgaccag ggcaattcgg cctggcattg
     1381 aaaaaggtgc cacaatcctc attggcggta aagcacccat aattaaaggg gcttaccttt
     1441 cctgggtact cgtgccattc atgaaacttg aaccacctta tgacgacctc aaggaccttc
     1501 tacgaaagat atgggattgg tgggatgaaa acgggaggac aagagagaga gttgctgaat
     1561 taatcgagag acttggattg aaaacctttc tgcgggaaat cggcctgaag cctgtgcccc
     1621 agatggtaaa gtatccacgg tccaacccat atgtgttttt tgataaatag accggagagg
     1681 aggccttgca atgggaaaaa ctgatatagg accacctgat ttctgggaca tgctcccaga
     1741 ggcaataaaa agaaattacg ggacgtggaa gtaccatgaa catgttcgcc caggtgtact
     1801 taaacacgtt tctgagagcg gtgaacagct ttacactatc agagccggat ctaccagact
     1861 cctcagtatt gatttcattc gtaacatatg tgacattgcc gatgagtttt gcgatggcca
     1921 ccttcgcttt acaagccgct ctaatatcga gttcatgact accgacgaat caaaagttga
     1981 gcagataatt ggcgcgctga atgcaatggg gctaccggtt ggcgggacag gaaattccat
     2041 ctccaatatt gtccataccc aggggtggat tcattgtcac agcgcggcaa cagacgcatc
     2101 aggcctcgta aaagccatta tggacggtgt gtatgaccat tttacagaca tgaagctccc
     2161 taatcatctc cggatagccg tggcgtgctg cataaatatg tgcggtgctg tccattgttc
     2221 tgacatagcc gtagtcggtg tccataccaa acctccaaaa cctgatcacg agcgggtcat
     2281 tgcacagtgt gagattccca ccacgattca gtcgtgccca acaggcgcca taaggcgcca
     2341 tcctgatgcc actatcaaaa gcgttgtcgt cagagaagat ctgtgtatgt attgcggcaa
     2401 ctgctataca gtctgtcctg caatgcctct tgcagatgct gagggagacg gcgtagcaat
     2461 ctatgcagga ggaaaagtag caaatgcccg gaaaccacct atgttttcga gaatggtcgt
     2521 gcccttcctg cccaataagc cgcctcgctg gcaagaggta gttgatatta taataaaaat
     2581 catcgatgtt tatagtaacg atgcaaagcc tgctgaacgg ctcggtgaat gggtagaacg
     2641 aataggttgg gaggcattct tcagaaaaac cagtataccc tttaccgagc agcacattga
     2701 cgacttcagc cacgcagtcg aaacttttcg ttcaaccacc atgttcaagt ggtaggagaa
     2761 aaaggaggtt atgccattgg cagatgagat tagcaacgaa ctgaaaaaca aaattgtgga
     2821 atacttaaaa acggttagca aggctaagaa caaagaggtc gcaaaggcaa tcgatactga
     2881 taaacatctt gtggataaag ctattgctga actcagtaaa gaaggcaaga ttgaatacat
     2941 ttatctcggc gcgtcattca ttaagctgaa ggacacatag gacatttgga caaatgtgag
     3001 gatcggtgca ggataaacag aatgtaacac cccgcattat gatcggggca cccaatggtc
     3061 gatctggaaa aaccatggtc agtgtgggct tgtgcgcact tttacacaaa aacggtcttt
     3121 ccgtacagcc tttcaagaaa ggacctgatt acattgatgc ctcatggtta acagcagcat
     3181 cagggaaagc gtgcagaaac cttgatgcat atctcatgtc aaaggatgtg ataatcagca
     3241 atttctgtaa atcttcgctg gggaatgatt ttgccctcat tgaggggaac atggggcttt
     3301 acgacggttt tgatggaggg gaggaaggta gctcggctca tcttgcaaga gtcctcaaaa
     3361 ctcccgttgt gttggtggtg aatacagcac gcatgactcg gagcatcgcc gcactcataa
     3421 aggggtatat ggattttgaa gcaggcacaa acgtgaccgg cgtcatcctg aacaatgtag
     3481 cgggacctcg ccatgaaaaa aaactctcag acgctgtgga acgttactgt aaaatccctg
     3541 tccttggctg tattcctcga aaccaagccc ttgcaattca tgagcgtcat ctgggccttg
     3601 ttccttttca ggaagcatcc agcggaacaa tagggattga acgtatcctt cgtacgattg
     3661 agaaacattt agatatcgaa agcctcatat ctattgcaaa aagcgcgcag ggcttgccta
     3721 ttagccattc aacaactcaa cagaggccaa aggtatcggt tcgtataggg gttatgatgg
     3781 accatgcctt ccacttctat tatccggaaa accttgaagc ccttgagcaa gaaggtgcgg
     3841 agcttgtcct tatcgatgcc atgcatgatg ataaactcga ggctatagat ggtctttata
     3901 ttgggggtgg cttccctgag ctttacatgt caaagcttca acttaatgaa accctgcgag
     3961 tccagattgc cacctccata gaggatggac ttccagttta tgcagagtgc ggaggcctga
     4021 tatatctgtg tgacagaatt gtaattgatg gagttcctta caccggcgtt ggagcgattc
     4081 cctcttcagt agaatttttc acgaaacccc aggcgcatgg atactcaaaa ttagaagtga
     4141 aatacgaaaa tcctttttac ccggtgggca tgcaaattta tggtcatgaa tttcattatt
     4201 cgaggctcac ctgtgcaaaa ggattgcact gcgctctcga attaacccga ggtcagggca
     4261 tagacggaaa gatggatggc attgtgtaca gaaacgtttt tgcttcttat actcatatcc
     4321 atgcctctgg tacttcaagt tgggcaaaat cctttgtatc cgttgcatcg cgaaataaag
     4381 gcatcggtaa atcagctttt gaacaagaat cacctcggga ggtgggtcat ggctgaaggg
     4441 acagaaacag tcaaatgtcc tgtatgcggg acagagattg aagttggttc acaggtggca
     4501 aaactttacg gtgctgtaag ggcagcgcca agggcaacgt cgagccacga aggcaaagag
     4561 tatcattttt gttgcaaggc ttgcaaaaag aagttcgata cagaaccgac acattatatt
     4621 aagaaatagg aggtcaggaa tggcaaaaac aactcttggc ggcatagaaa tcgaagttga
     4681 tgaagatgga tttatacagg aaccagagaa atggaataag gaggtagccg aagacctgtc
     4741 gaaattggaa caggcttttc ctatgggaga ggaccactgg aaaattgtta attacctcag
     4801 ggattattat ctaaaatatg agattgcgcc tcctattcgg atgctggtaa aacagacagg
     4861 gattgacctc aagactatct atcagctttt cccaggtgga ccggcaaagg gcgcctgcaa
     4921 ggttgccggc ttgcccaaac caacagggtg cgtgtagggc acataaggca cggtgattat
     4981 ttcggagtgc tttgttcgaa gccaatactt tttcgaagtc ggatcttggg ctgaccatgg
     5041 acatctttct cattctgctt acctgtttcg cctatatctt tattcttgtt gcctacagcg
     5101 cgaagattgt gaaatatgcc cgtatgccga ttcatctgag atgggagcta ttcacggcaa
     5161 caaaaacaga aaaaaaacct tatcaccatg gcttaacagc attcgaagcc cagcctttcc
     5221 agtcaagcag gtggagatta tccaatgtgt ggtctttttt gaaggagtac ctgtggtttc
     5281 attcttatct gtcacatgat aagacttact ggtgctttct ctatattgcc catataggct
     5341 ttgtgagtct gattatattt caagcggtat gcattgccac agccctggga accttcctgg
     5401 cacatgatca aaccatcact gctgaaaggg ctacaggcag ccttgtagct cttagaattc
     5461 tgctggccat aatcagtttt gcagcaggca taatcggaaa tattggtcta atggtttaca
     5521 ggatcagcca tcgtgatttg cggctttaca cccccccctt gacctttatt ggctactgta
     5581 tatccatatg cacctgctta atcggcttca tcctcttgct gacaaacgat cctcattttt
     5641 ccggctacgt gttgtttttt gttggcatgt tccaaataaa gccaatagcg gtctcaggcc
     5701 ttctgggact gttcatattc cttaacatac ttcacctgat ctatcttcca tttacaccag
     5761 gctttcacta tgtcacaagg ttctttgcct tttttaacgt tctatgggac accaggccaa
     5821 accaaaaagg aagtccagta gagaaagcga tcggtcaagt catggggaaa aaaattacat
     5881 ggtccgcgcc tcacattaag tcaggacaga catgggaaca agcagcaaag cacagaagtt
     5941 gagaagatag aaaacagaga aattcagaag agataaagat aattacacat cgaaaatgct
     6001 atgagtaaag aatcgggaaa agaaaaacag gactaagcat gagaattgaa gacataagta
     6061 aaaaatctga tcagatcatt aaaataaggc aatctgacct attgcccttg ccttaccctt
     6121 acaacaaccc tgaaaaagat actgccatga gagagctttc gtcagaccag agggaacggt
     6181 acgaagcgtc ccttgatgga gttatcggag ttggtattca aagaccatcc agcaaagcag
     6241 aagaagaatt attcattcac aagttcctct ctggcctggc aaagcttttt gaaaaggaaa
     6301 ataactggac cttcctccat cccctgtatc attcccttga atattgtgtc aaatgtcaga
     6361 tctgtaacga ttcctgtccg gcttacattg cgagtggtag aaaggaaatc tacaggccta
     6421 cttataggcc tgaagtgctg agacgcatag ttgacaaata tataaagaag aaaaatagag
     6481 caagtctgaa attaacagga agcgacattg aactcaactg gacgctcatc gcccgccttg
     6541 gtgaactggc ctatcggtgt actctgtgcc gtaaatgcac gcaggcatgt ccgctgggga
     6601 ttgacaatgg actcatcagt cacgaaatca gaaaggtttt cagtcaggag atggggatag
     6661 cccctaaaga actgcacgaa caggggaccg tccagcagct taaagttggt tcgagcacag
     6721 gtatcaaccc cagggccttg gaaaacatta ttgaattcat ggagaatgat attgaggaaa
     6781 aaattggccg taagattaag atccctgtgg ataaaaaagg tgctgaaatc cttctcctgc
     6841 acaatgccgg tgagttcatg tcctggcctg aaaacactga agccttcgca attattttcg
     6901 atgcagcagg aatagactgg accctttcaa gcgagaccgt gggatacgat gcggtcaact
     6961 acggtgtctg gtatgacgat atacaacttg cccgtgttgc cacaaggcag gctgaaatag
     7021 cacggaatct gggagtaaag aaaatcaatg tgggcgaatg cgggcatgcc cacaaggcaa
     7081 tgatcgttgt tgccgacagg gtgctcacag gcgatctaaa catcccaagg gaaagtgccc
     7141 tgcctatact tcgcgacata gttgtgggag acaaaattaa gctggactct tccagaaaca
     7201 attttccaat aacccttcac gatccttgca acatggtaag gcttatggga atagtagaac
     7261 cacagagaga aatattgaaa aagatctgcc ctcaattttt ggaaatggaa cctcacggcg
     7321 tagataatta ctgttgcggg ggtggaagtg gattcgcaat catgcagtcc atgaacttcc
     7381 ctgactggcg aagtgctgtc tcggggcgta ttaagcttaa gcagatactg gaagtttttc
     7441 aggataccat ttcaccggat gtcaaaaaat atgtctgtgc tccatgctcc aactgcaagg
     7501 gacagatacg agatcttctt aattattaca atgtctggga tgaatgcggc attttttatg
     7561 gaggccttgc ggaactcatc gtcaatgcca tgatagatct tgaaaaacca ttcatcgagt
     7621 gggagtggca ttgaaaaacg gctttgagtc attaatatta agctttgaag ggctgtttca
     7681 cgaaagggat tttgtatcgc tatgagcggt aaaacataaa ttgtcaataa aattggaggt
     7741 gatagcatga agggaaaagt aattctgtta aattccgagt atataggcag ggaggatgag
     7801 acgcttggat ttgagattct catgacgctg cttaacagtc ttttgaagag aaatgacaag
     7861 cctgaagcca taatcctgtg gaatacaggg gtgaagctca tgacatcaca atcaccagcc
     7921 tccgcactcc tccgggagct tgaactggca ggcgtgaaaa tactcggtgg ggtgctttgc
     7981 ctcaacgatc tctgtatagc agatgcagtt tcagtcggac aggctgtgac aatggatgat
     8041 atacttgact tgatccttca taatgaagta ataagtttat aacatttaga aacaaaatca
     8101 tgggttcacc agtatcatac aaggagagag aatgggaaaa ggcgatcaac gaacaaaacg
     8161 gggcaaaata gcacgaggaa cgcacggaaa aaccagaccg aaaaagaaag gaaagaaagt
     8221 aagtaaataa gggggtatat gggccccaaa ggtataggca tcagatagct gttgatagga
     8281 aacaatgctt ttgcttcttt ctattgccta tagcccatta cccataccca gatattttta
     8341 cgaggtaata tgggaatttt acaagacatt gaaagattgg gccttaagct tgatgatgac
     8401 gcccttgaac acatcaaaat gatacaggaa gacatagtac gcatgaacca ggacgaactg
     8461 gttcgaaata cgctcataga gatgcatatc tcagaattta tcgaaatggc aagacctgac
     8521 atcatgactg ctcttttgga tgataaaaat agggaggttg agcaacttac aaaggaattt
     8581 cagcggaagt ttgatagcca aggcaacaga atttgtgagg agcacacgtg tgactcaata
     8641 attgaagtcc atcggtgcac ctattgtggt aaatatgtgt gcaaagaaca caattatggc
     8701 agcgaggatc ttcgttgctg ctatgattgc tcaatcgcgc gcacctatgg caaaaaataa
     8761 ttattcccaa aacctatagt tacactttac atacctgtac cataagccgc catacagctt
     8821 tccctcattt gccttcacag tattgttact aatacagttt ttaactcgcc tccacatttt
     8881 ctgttgattc aggagcccta aatccagtgc aaaagaatgg ttcaaccatc tttagtgttt
     8941 cgaatatcca ggtatacggg tttcttataa agacacctgt atggtgttat ctttattgcc
     9001 caggtacatt aatgggaggg aaccgtacac tctcctagag gataacatgg atgagggttt
     9061 ctgtatggaa ttcaaacccg actgcctttc tcctgcacca gacaggggtg ctcaaaagga
     9121 attgctgtgg accgaaacaa tatgccccat atttcccata cgagatcagg aggacaggta
     9181 agcttcatac gttatgattg caccgcgtgg ataagtgaga ctattgtcga aaatgctttc
     9241 tattgtggag taacgtcact ggcagttact ccaggggaac agcccatata acaaccttca
     9301 gcgatgattt tctgccaaga ctgctcttcc tcgcaacaaa tgtatcaggc ttacgggagg
     9361 gaggtgatct atctggtcga tgggcagaca atcttccctt gccctaaaca cacttggcca
     9421 tccacatatt gcctattttt cagaaaacgc aggtgagttt aaatataccc gctacagtgg
     9481 atccgactgg ataaccgtta tcttggagta tcctcgcaga agcgcccagc attgccctgg
     9541 acaaactcga ccaatcatac ataagctttg gtacgtcaat tgatggttac gttgaggtac
     9601 tcatatactg ctatcataac ggacaagaaa agtcttcccc gagttatatt caggtactgt
     9661 caacttaatt gtgtaaattt tttatagagg ggcaaattcg gacgca
//
LOCUS       DAOOMC010000107         9657 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdus sp. isolate AMR_MDS_2685
            NODE_8203_length_9657_cov_6.047490, whole genome shotgun sequence.
ACCESSION   DAOOMC010000107 DAOOMC010000000
VERSION     DAOOMC010000107.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864760
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdus sp. (wastewater metagenome)
  ORGANISM  Syntrophorhabdus sp.
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae; Syntrophorhabdus.
REFERENCE   1  (bases 1 to 9657)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9657)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,729
            Genes (coding)                    :: 2,708
            CDSs (with protein)               :: 2,708
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 7 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9657
                     /organism="Syntrophorhabdus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8203_length_9657_cov_6.047490"
                     /isolate="AMR_MDS_2685"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2699756"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(497..1330)
                     /locus_tag="PK125_10555"
     CDS             complement(497..1330)
                     /locus_tag="PK125_10555"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPB38583.1"
                     /translation="MNFLRRITNHNLALVGLLILIPMFICAVTAPIISPHDPVEPDLK
                     NILDSPSWSHPFGTDTLGRDVFSRVIYGSRISLLVGFVSVGIATLIGIMVGALSGYSG
                     GIVDELIMRFVDLMMCFPTFFLILAVIALLEPSIWNIMIVVGLTNWMGIARLVRAEIM
                     SIKNKEYVLAAKAMGFPNRRIIFGHVLPNSLSPVYVVATLGIGGAILTESALSFLGIG
                     VQPPTPSWGNILTQAKDNIEVAWWLSLYPGLAIFLTVMGYNLLGEGLRDIFDPRRRYQ
                     T"
     gene            complement(1410..2390)
                     /locus_tag="PK125_10560"
     CDS             complement(1410..2390)
                     /locus_tag="PK125_10560"
                     /inference="COORDINATES: protein motif:HMM:NF024640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terpene cyclase/mutase family protein"
                     /protein_id="HPB38584.1"
                     /translation="MKKVIFLTFLFIIVVSFTVFAAPQDTLRKAMVHIVTMQDENGTW
                     SRLKGEFPPEAEPTALAVKVLTMNGMALEKAEKGVAFLLKDQRPDGSWGNNTAGTAFT
                     IIALKQANTGEDAINKGIQYLRDVQDNNGGFKRLGKEGAPISFYTAVVLTALKEAEVP
                     RNDPAVRMAIDWLMGCQNTDGGYPMLKGTPSIGPATAWVVKALLAYGAAPTTPFVQDA
                     VDYLVTTQKSQGGFAPALPAAEDPEATAYALMALSKYPDKKMNVHKGTEYLGKAQQPD
                     GAFISASPIQFNKIAKKNTQTTCFVAWALSELAGKGAGGTPTTPLDLRVK"
     gene            complement(2517..3929)
                     /locus_tag="PK125_10565"
     CDS             complement(2517..3929)
                     /locus_tag="PK125_10565"
                     /inference="COORDINATES: protein motif:HMM:NF014910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HPB38585.1"
                     /translation="MDKESVDVFINQIRGFLKEEQIITDKDGMSHFCYDATEMRFMPD
                     IVLLPCSSEEVSKIMKLASRLDLPVTPQGGRTGLSGGALPVHGGLVLSLLRMNKILEI
                     DQKNMQIVVEPGVIAEDLQIELKKYNLFFPPDPSSTVESTLGGNVAENAGYTRAVKYG
                     VTRDYVLGLEAVLPDGEIINIGGRTVKNVTGYDLVALLIGSEGTLAIVTKIICKVLPR
                     PAIRKTCIMYLDDLGAAADLVVTIFGAGILPCAIEFMDSMSINCVADYLPTAESLIRR
                     DAEALVLVEVDGNHEIATDYEARRIMDLSKGFSGVIEAKYAENDEQAEKLWRIRRDTL
                     PALKAKGKDHLEADVVVPRYRLPFLVRAIQESVATKTVKVATFGHAGDGNLHVTIQYR
                     KRNFAELEEANELLGDIYRKAIEMGGKLTGEHGVGITAKDYMKIQMSPAEIALMKRIK
                     YAFDPKGILNPGKIFPEESG"
     gene            complement(4007..5299)
                     /gene="larA"
                     /locus_tag="PK125_10570"
     CDS             complement(4007..5299)
                     /gene="larA"
                     /locus_tag="PK125_10570"
                     /inference="COORDINATES: protein motif:HMM:NF033504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050043 - lactate racemase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-dependent lactate racemase"
                     /protein_id="HPB38586.1"
                     /translation="MVMHITMHYGKTGLPLEFPDAWDITIIRKKPMPSIQNPEISLKS
                     AFENPTGGHPLTELSRKCNNACILICDITRPVPNGVILPTLIRQLIDGGIKPSNILIL
                     VATGLHRPNEGQELLEIVNSKWVMDTVKIQNHFARRDEDHEDFGKTSRGTVIKLDRRF
                     VHANLRIVVGLVEPHFMAGYSGGRKMVAPGIAHRDTITSIHSAGMLEQKGVENCTIEE
                     NPLHQELTEIVKKVGECFAVNVVIDEERKISFLNFGDIEDSHREAVTFVRSFAEIPVT
                     QRFKTVVTSGAGYPLDKNYYQTVKGMVAAIDILEPGGNLFIVSECSEGIGSPEFVECQ
                     RRLIEFGPEAFMKEILSKTMADIDEWETQMQLKVAKVGRIHLYSGCLCDEDMRLTGVE
                     PVQSVMDAVQRSMDTFRDNRVAVIPEGPYVIPRRNAAR"
     gene            5514..6683
                     /locus_tag="PK125_10575"
     CDS             5514..6683
                     /locus_tag="PK125_10575"
                     /inference="COORDINATES: protein motif:HMM:NF020252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HPB38587.1"
                     /translation="MDVQADLIRRLESGFSLPTLSMVAIRLVELASDEECSVNDLTSL
                     IEKDPSLAVRLLRIANSAFFKSVEPAKTLKQAVIRIGFQQLRIMALSISLRDTFPMGI
                     VKGFDYERFWRASLYRGLMTKSLAEQIKNCNPEEAFVAGLTLGVGFLIFFNMFIKDSG
                     EEIDHRLDSLQKLLVWEQEKFGVNHRQIGAAALHYWKFPDSIVECQEGFLRLPDSSPL
                     SSICELARNMSMSLLGKSKDFHDLYRDAKDWFGLGGSAINVVILSVFQEVDAIAENLK
                     IEMDKEKDLLGLMEKANRALSSISEKISMMQLQGGGIEPPSFETIKEGKGGQDVVSHT
                     LQAVAHEIRNPLIAVAGFARRLSTTLDPESKGGKYAQIILQEAQRLEEALSQMKA"
     gene            complement(6664..7272)
                     /locus_tag="PK125_10580"
     CDS             complement(6664..7272)
                     /locus_tag="PK125_10580"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: protein motif:HMM:NF024214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0061603 - molybdenum cofactor
                     guanylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase"
                     /protein_id="HPB38588.1"
                     /translation="MDVTCAILAGGKSTRMGKDKATMKVCDKPLIQLVYEKVKPVFKK
                     IIIVSSVHDQFPGIDAPIVRDIAPFRSSMVGIASALLSADTPYVFVLACDMPFVTEQS
                     IGYMLKAVHGEEVVIPRTQYGWEALHAVYNRSCLSVMLTHIWFNRLKIIRTLPFFSVR
                     EVREHPAFINGKISVFTNINVEEDLGLVEDLNNISCVTPSSG"
     gene            7479..7889
                     /locus_tag="PK125_10585"
     CDS             7479..7889
                     /locus_tag="PK125_10585"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(deoxy)nucleoside triphosphate
                     pyrophosphohydrolase"
                     /protein_id="HPB38589.1"
                     /translation="MKNKPNPKIVVAAVIEVEGCILIGKRKQGKMHPGKWEFPGGTLE
                     EGETPEQCLKRELLEELAVITEIGDLICTSEYIYTPDWVIRLLVYKTTVVSGSFNLNA
                     HDEIRWVRPADLAAYDFPEADRPIVEKLIREGCR"
     gene            complement(7911..8495)
                     /locus_tag="PK125_10590"
     CDS             complement(7911..8495)
                     /locus_tag="PK125_10590"
                     /inference="COORDINATES: protein motif:HMM:NF014693.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formylmethanofuran dehydrogenase subunit E
                     family protein"
                     /protein_id="HPB38590.1"
                     /translation="MSVHVLEYTFEEYTEIVKKFHGAAAPGVLIGGFMVDAAIRNLPD
                     GILYDAICETKSCLPDAIQLLTPCTTGNGWLTVIHLGLFALTLYDKSTCQGVRVFLDP
                     EKVESWKEIKAWFFKIKSKKEQVLELINKEIEAAGTTILSAQKVEILPEAAQKKHKGK
                     IGLCPKCREVYPLDHGGICLLCQSNTLYAHGTHI"
     gene            complement(8855..9070)
                     /locus_tag="PK125_10595"
     CDS             complement(8855..9070)
                     /locus_tag="PK125_10595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB38591.1"
                     /translation="MRILLGRVERGINDASIVRKSLLPSADHFAVCTEGFFRCLLSIG
                     FIEPYDLHPLLHYRTLLKIPQAIEHFL"
     gene            9069..>9657
                     /locus_tag="PK125_10600"
     CDS             9069..>9657
                     /locus_tag="PK125_10600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB38592.1"
                     /translation="MPHIHWKVVAYMRSLRETYPGIGKEKIKPLLDAYCEKEGLKTIS
                     ISSIGNTMKRHHLFFHTTQKVYHDPSSGWAHRKTTEKRTKVKHSPKPKEFGYVISDTI
                     GRITDGIKDYFYNAMEIKSRFALTLHYKRLSRKTMQEFSEQFQMIYPGEIHTWQTDNG
                     SENLGDFHKYLKKDGILYPRCPKIKDTVLLEVFMEI"
BASE COUNT         2672 a         2159 c         2170 g         2656 t
ORIGIN      
        1 cagtagtggt ccgagaaagc tgctcgagga aatgcaggag atcaggcccg ttgacgccct
       61 ctttgaaagt gatgatccat agttcctact tctggaatct gctactacgg agaatgaata
      121 ttttttatca ggagctggat tcaaaaggtt gccacttttt ctattaccgg gaaaagaaaa
      181 agtcaggttg tttaggcact gcgaattcat ttttgaccat tcgcataaga taaacaactc
      241 cttgaaataa tcgattataa gttggttgag atgttaggat aagcatgcgc cgggatttcc
      301 ctgaaaatat attcgttcac tccagcagtg acttttactc gagtttgtat tcgtgacttt
      361 tgctgacgca aaaaaagcca tgcattcttg tccgttaaca gttgacataa gaaaattcga
      421 catgatgctt caatggctta tctgattaaa aaagttatta aaattattta actttgatct
      481 gcgttgttgt tgatcgtcat gtttgatagc ggcgcctggg atcaaagata tcacgaagac
      541 cttctccaag aagattatag cccatgactg taagaaagat ggcgagtcca ggatagagag
      601 aaagccacca ggctacttcg atattgtcct ttgcctgggt cagtatgttg ccccaactgg
      661 gtgttggcgg ctgaactccg atcccaagaa agcttaaagc ggactctgtg aggattgcac
      721 ctccgatacc aagtgttgcg acaacgtaaa cgggtgataa tgaattgggc aaaacatgtc
      781 caaaaatgat tctcctgttt ggaaaaccca tggccttggc agcaagaaca tattccttgt
      841 ttttaatgct cataatctca gccctcacta ggcgggcaat gcccatccag ttcgttaggc
      901 caacaacaat cataatattc cagatgctgg gttcaagtaa ggcaattaca gcaaggatga
      961 gaaaaaaggt aggaaaacac atcataagat caacaaagcg cataatgagt tcatcaacga
     1021 tgccccctga gtaacctgaa agtgccccga ccattatgcc tataagcgtt gcaatcccta
     1081 cagacacgaa accgacaaga agggatatcc tgctcccata aattacccgt gaaaacacat
     1141 cacgccccag ggtatctgtg ccgaaaggat gtgaccatga cggcgagtca aggatgtttt
     1201 ttagatcagg ttctacgggg tcgtgagggg atataatagg cgctgtaact gcacagataa
     1261 acatgggaat cagtatgaga aggcctacta atgcaagatt gtgattggtt atgcgtctaa
     1321 gaaaattcat ggaataaaac aataaaatga aggagttatg aattgaaacc tgcgctcgtt
     1381 tagttcgtaa caacttatga ataattgttt catttgactc tcaaatctaa gggtgttgtt
     1441 ggtgttcctc ctgctccttt gcctgccaac tcgctcagag cccatgccac aaaacaagtt
     1501 gtctgggtgt ttttcttagc aatcttattg aactgaatcg gagacgcgct gatgaaggcc
     1561 ccgtcaggtt gttgtgcttt accaagatat tcagtgccct tatggacatt catttttttg
     1621 tcggggtact tgctcaacgc catgagtgca tatgctgtag cttcagggtc ttctgcggca
     1681 ggaagggccg gtgcaaagcc tccctgggac ttctgagttg tgacaaggta atctacagca
     1741 tcttgaacaa atggtgtcgt gggtgctgcc ccataagcaa ggagtgcttt tactacccaa
     1801 gctgtggcag ggccaatcga aggcgtaccc ttcagcatcg ggtaaccacc atctgtattc
     1861 tgacatccca taagccagtc tattgccatt ctcaccgctg gatcattccg gggaacttct
     1921 gcttccttta aggcggttag aacgactgcg gtgtaaaaag aaattggagc cccttctttc
     1981 cccagtctct tgaagcctcc attattatcc tgaacgtccc gtaggtactg aatccctttg
     2041 ttgattgcat cttcacccgt gttagcctgt ttgagggcta tgatagtaaa agctgtgccc
     2101 gctgtattgt tgccccaact cccatccggc ctctgatctt tcaggaggaa ggccacacct
     2161 ttttcagcct tttccagggc cattccattc atggtgagca cttttaccgc caatgccgtt
     2221 ggttctgcct ctggcggaaa ctcacctttt aatcgcgacc atgtgccatt ctcatcttgc
     2281 atagtcacaa tgtgaaccat tgcctttcta agggtatctt gaggtgcagc aaacacagtg
     2341 aaagaaacca ctataatgaa aagaaatgtc aaaaagatca cctttttcat gctgacctct
     2401 tgctgttttt tctttttata gcactctaag attggtgttg caacagtaat aatgaaagga
     2461 acaggctaaa gccaggagac aatagattta aacccgtagc ccattgccag aaagagttat
     2521 ccgctttcct ccggaaatat ctttcccggg ttgaggatgc ccttagggtc gaaggcgtac
     2581 ttgatccgtt tcatgagagc aatctctgcg ggtgacattt gaatcttcat gtaatccttt
     2641 gcagtaatcc ctacaccgtg ttcaccagtc aacttgcctc ccatttcgat agcctttctg
     2701 taaatatcgc caaggagctc attagcctcc tccagctcag caaaattcct tttacgatat
     2761 tgaatggtaa catgaagatt gccatcccct gcatgaccga aggtagctac tttaaccgtt
     2821 tttgtagcaa cactttcctg aatggctctg acgagaaagg gcaagcgata tcgaggcaca
     2881 acaacatctg cttcgagatg atctttgccc tttgccttga gggcagggag ggtatctcta
     2941 cggattcgcc acagtttttc agcctgttca tcattttctg catactttgc ttcaatgaca
     3001 ccggaaaaac ccttactcag atccataata cgacgggctt catagtctgt agctatctca
     3061 tggttgccat ctacctcaac aagcacaagg gcctcagcat cgcgccttat gagcgattcc
     3121 gcagttggca gataatcggc cacacagttg atgctcatgc tgtccatgaa ctcaattgca
     3181 cagggcagta tgccggcgcc aaatattgtt acaaccaggt ctgctgcagc cccgagatca
     3241 tcgaggtaca taatgcaggt tttcctgatg gcgggtctgg gaagtacctt gcagatgatc
     3301 ttggtgacaa ttgccagtgt tccctcagaa ccgataagaa gagcaacgag atcgtatcct
     3361 gttacgtttt tgacagttct gccgcctata ttgataatct ctccatcagg tagaacagct
     3421 tcgaggccaa gcacgtaatc ccgggtgacg ccgtatttta ccgcccttgt atagcctgcg
     3481 ttttcagcaa catttccacc caaagtgctt tctacagtac tcgatgggtc aggaggaaag
     3541 aagaggttat actttttcaa ttctatctga aggtcttccg caataacacc gggctctacc
     3601 acgatttgca tgttcttttg gtcgatttca agtatcttgt tcatcctgag aagtgagagt
     3661 accagtccgc catgaacagg gagtgcccct cctgagagac cggtccttcc accctggggc
     3721 gtaacaggca aatctaacct gctagccagt ttcataatct tcgacacctc ttcagaagag
     3781 cagggcagga gaacaatgtc aggcatgaac ctcatctctg tggcatcgta acagaaatgt
     3841 gacataccgt ccttgtccgt tatgatctgt tcttccttga gaaatcctct gatctggttt
     3901 atgaacacgt caacagattc cttgtccatc tgctctccgc catacttgct gcaaaatcat
     3961 taacgcatta agccccgaat taattactga taaatctgaa ccacagttac ctggccgcat
     4021 tgcgtcgagg tatcacatat ggcccctccg ggataacagc cacacgattg tccctgaatg
     4081 tatccatgct ccgctggaca gcatccatga cggactgaac aggctcaaca cctgttagcc
     4141 tcatgtcctc atcacacagg caaccactgt aaaggtgaat tctgcccact ttagctactt
     4201 tgagctgcat ctgtgtctcc cattcgtcga tatctgccat tgtttttgac aaaatttcct
     4261 tcataaaggc ttccgggcca aattcgatga gccgtctctg gcattcaaca aattcaggtg
     4321 aaccgatacc ttccgagcac tctgatacaa tgaaaagatt tcctccgggc tcaagaatat
     4381 cgatggctgc caccataccc ttaactgtct ggtaatagtt cttgtccagg ggatatccag
     4441 caccgcttgt aacgactgtt ttgaacctct gagtaaccgg gatttccgca aaagaacgaa
     4501 caaatgtgac tgcttccctg tggctgtcct caatatctcc aaaatttaaa aaagatattt
     4561 tgcgctcctc atcaatcaca acgttcactg caaaacactc tcctaccttt ttgacaatct
     4621 ctgttaattc ctgatgaagg gggttttctt caatcgtaca attttctacg cctttttgtt
     4681 cgagcatacc ggctgagtga attgaagtta tagtatcgcg atgggctatg cctggggcga
     4741 ccatctttct tccacctgag taaccggcca taaaatgagg ttcaacaagg cccacgacga
     4801 tgcgaagatt agcgtgtaca aagcgtctgt caagctttat tacagtaccc cgactggttt
     4861 ttccgaaatc ctcatggtct tcatcacgac gagcaaagtg gttttgtatc ttaaccgtgt
     4921 ccattaccca tttgctattc acaatttcaa gcagttcctg gccttcattt ggccggtgga
     4981 gacctgttgc cacaagtata aggatgttcg agggctttat gcctccatcg ataagttgtc
     5041 ggattaatgt tggcagaata actccatttg gaacaggtct ggtaatatca cagataagta
     5101 tacatgcgtt gttgcacttc cttgacaact cagtgagggg gtgccccccg gtaggattct
     5161 caaaagctga cttcaaggat atctcaggat tctggataga tggcataggt ttttttcgga
     5221 ttatcgtgat gtcccatgca tcaggaaatt caagtggcaa accggtttta ccatagtgca
     5281 ttgtaatgtg catcaccata ctctctttct caaaaagtat acgtttctta cgggaatctg
     5341 tcaaagaata taggtgagaa tggcagggcg cgcgggaaag accgtgacga acaatgccta
     5401 atccgcagtt ggtttataac ctatcactct tcactattcc ctgatctatg gttgtttgtc
     5461 aatattttct gattgtattc caaaacttta gctgttatat acatgagaca gtgatggatg
     5521 ttcaggcaga tcttatcagg cgtctggaat ctggattttc tttacccacc ctttctatgg
     5581 tggcgatcag gctcgtggaa cttgcttcag atgaagaatg ttcggtcaac gacctcacat
     5641 ctctcattga aaaggatccc tcccttgctg tcagactgtt acgaattgcc aatagcgcct
     5701 ttttcaaaag tgtcgaacct gcaaagaccc taaaacaagc ggtgattagg attggatttc
     5761 aacagttacg tattatggca ctttctatat cgctcaggga tacatttccc atgggtatcg
     5821 taaaaggctt tgattatgaa aggttctgga gggcgtcact atacaggggt ttgatgacaa
     5881 aatctttggc agagcaaata aagaactgca accctgaaga ggcttttgta gcgggcctta
     5941 cccttggggt tggctttctg atctttttta atatgtttat caaagactcc ggagaagaga
     6001 tagatcatag attggatagc ctacaaaaac tcctcgtatg ggaacaggaa aagtttggtg
     6061 tgaatcatcg tcaaataggc gcagcagcac ttcattactg gaaattccct gattcgattg
     6121 tagaatgtca ggagggattt ctcaggcttc ctgatagcag ccctttatcc agtatttgcg
     6181 aacttgcgag gaatatgtcc atgagtctgc tcgggaaatc gaaggacttc catgatcttt
     6241 atcgtgatgc aaaggactgg tttgggctcg gaggaagtgc aatcaatgta gttatcctgt
     6301 ctgtgtttca ggaggttgac gcaatagcgg aaaacctcaa aattgaaatg gacaaggaaa
     6361 aagatcttct tggtttaatg gagaaggcga accgtgccct gagcagtatc tctgaaaaaa
     6421 tctccatgat gcaattacag ggaggaggaa ttgagccgcc ttcatttgag acgattaagg
     6481 aagggaaagg aggtcaggat gtggtcagtc ataccctgca ggcggtagcc catgagatca
     6541 gaaaccccct tatcgcagtg gctggttttg cgagaaggct ctccaccacc cttgaccctg
     6601 aatcgaaagg tggaaaatat gctcaaatta tactccagga agcccagcgc ctcgaagaag
     6661 cacttagcca gatgaaggcg taacgcaact aatattattc aaatcctcaa caagacccag
     6721 atcctcttcc acatttatgt tggtaaatac cgatattttt ccatttataa aagccggatg
     6781 ttcccggacc tctctaacgg aaaaaaaggg aagagttcga ataattttga ggcggttaaa
     6841 ccatatatgg gtgagcatga cagaaaggca tgatcggttg tacactgcgt gaagggcttc
     6901 ccaaccatac tgggtgcgag ggattacaac ctcttctcca tgaaccgctt tcagcatata
     6961 cccaatggat tgttcagtca caaatggcat gtcgcaggca aggacgaaaa cgtagggtgt
     7021 atctgccgag agcagggccg aggcaatgcc taccatggag gatcgaaagg gcgcaatgtc
     7081 tcgaacaata ggggcatcaa tgccgggaaa ttgatcatga acactcgata caataatgat
     7141 ctttttgaat acgggcttta ctttttcata aaccagttga atcagcggct tatcgcatac
     7201 tttcattgtg gccttatctt tgcccattcg ggtacttttt ccaccggcga gtattgcgca
     7261 cgtaacatcc atgacaggta tttatcatct ttttgaaaat gagtaaagaa acagaacccg
     7321 ctttctatat tcttgtttat atcagtagtt tatttatgct atttttcatg attatgagag
     7381 catggtttcc aataagaaac gctgaagcag gtttttcgac aatagaggtt gtcatcaaag
     7441 agatgattca agcagtaagg agagtctgac agggtgtcgt gaaaaataaa ccgaatccaa
     7501 aaatagttgt ggctgctgta atagaggtag aaggctgtat acttattggg aagagaaaac
     7561 aaggaaagat gcatcccgga aagtgggaat ttccaggtgg cacccttgaa gaaggggaga
     7621 cccctgagca atgcctgaaa agggagctcc tcgaagagct tgcggtaata acagaaattg
     7681 gagatctcat atgcacaagt gagtatatct atacacctga ttgggtcatt agattgctgg
     7741 tttataaaac aaccgttgtc tcgggcagtt tcaacctcaa tgctcacgat gagatacggt
     7801 gggtaaggcc tgcagacctt gctgcgtatg attttcccga agcagacagg cctattgtag
     7861 aaaagcttat tagagagggt tgccgttaaa atattattgg tttgttggtg ttaaatatgg
     7921 gtaccatggg cataaagagt gttactttga cagagaaggc agataccgcc atggtccagg
     7981 ggatatactt cacggcattt ggggcatagg cctattttgc ctttatgttt tttttgagct
     8041 gcttcaggca gtatctcaac cttctgcgca ctgagaatag tggttcctgc cgcttctatt
     8101 tccttattaa tgagttccaa tacctgttct tttttagatt ttatcttaaa aaaccatgcc
     8161 ttgatttcct tccatgattc taccttctca ggatcaagaa agacccgtac tccctgacag
     8221 gtggatttgt cataaagagt taatgcaaaa agccctaagt gaattaccgt gagccagcca
     8281 ttacctgtgg tgcatggcgt caataactga atggcatcag gaaggcagga cttcgtttcg
     8341 cagatggcat cgtaaagaat gccatctgga agattccgta tggcagcatc aaccataaat
     8401 ccaccaataa gtactcctgg ggctgcagct ccgtggaatt tttttactat ctctgtgtat
     8461 tcttcaaatg tgtactcaag aacatggacg ctcatttcag attaaacttt cttaaaagtt
     8521 gcttttgtat ggcaatagat agcataaagt ctgtataaga aaatgtcata agaacaggta
     8581 aaattataaa gaaagttgtt gagaattaca aacaaccgtc aatgctttgt ttgaagatat
     8641 ccggttttac catgcttgta taattgactg gagcatatga ccagtctctt gcggtcacgg
     8701 agcgccacat caggtagttt cacttgttgc gaattttctg ctcatataca gatatagggg
     8761 tgaaaaagtt taaatacatt tgcgacattc atggttaatg gatcatcatg ggaaagcata
     8821 cccagtattt agtagaatac aaggctacag ggggttacaa aaagtgctct atagcctgcg
     8881 gaattttgag gagagtgcgg tagtgaagca gcggatgaag atcatacggt tctatgaaac
     8941 ctatggagag aaggcaacga aagaagcctt cggtgcagac cgcaaagtga tcagcagatg
     9001 gaagaagaga cttacgaaca atggaggcat cgttaatccc tctctctact cgtcccaaaa
     9061 ggatacgcat gccgcacatc cactggaagg ttgtcgccta tatgcgatca ttgcgggaaa
     9121 cctatccggg aatcggcaag gagaagatca agcctctcct ggatgcgtac tgtgagaagg
     9181 aagggttaaa gaccatctcc atatcctcaa tcggcaatac catgaaacgg catcatctgt
     9241 tcttccatac cacacagaag gtttaccatg atccctcatc agggtgggca cacaggaaaa
     9301 caacagagaa acggacgaaa gttaaacact cccctaaacc caaggagttt ggttatgtca
     9361 tctcagatac catcgggagg attacggatg ggatcaaaga ttacttctac aacgctatgg
     9421 aaataaagtc ccgctttgcc ctgacccttc actacaagcg cctctcccgt aaaaccatgc
     9481 aggagttctc cgaacaattc cagatgatct acccgggaga gatccatacc tggcagacag
     9541 acaatggctc agagaacctc ggggatttcc ataaatacct caagaaggac ggtatccttt
     9601 atccccggtg tccgaagata aaggataccg tccttcttga ggtatttatg gaaatcc
//
LOCUS       DAOOMC010000108         9525 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdus sp. isolate AMR_MDS_2685
            NODE_8362_length_9525_cov_5.865681, whole genome shotgun sequence.
ACCESSION   DAOOMC010000108 DAOOMC010000000
VERSION     DAOOMC010000108.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864760
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdus sp. (wastewater metagenome)
  ORGANISM  Syntrophorhabdus sp.
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae; Syntrophorhabdus.
REFERENCE   1  (bases 1 to 9525)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9525)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,729
            Genes (coding)                    :: 2,708
            CDSs (with protein)               :: 2,708
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 7 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9525
                     /organism="Syntrophorhabdus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8362_length_9525_cov_5.865681"
                     /isolate="AMR_MDS_2685"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2699756"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            93..1010
                     /locus_tag="PK125_10605"
     CDS             93..1010
                     /locus_tag="PK125_10605"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HPB38593.1"
                     /translation="MERKKKWQTHRGFIQDQNKGFDVIECEKCGFKHIVPIPTKEELQ
                     SVYQNDYYSMVKPLYIERYREDLGWWNIVYADRYETFEECLPTKFQRKMLDVGSGPGF
                     YLLHGKERGWETLGIEPSEKAVAHSRSLGLNIIEGFLTSDNFKDMGTFDVIHMSEVLE
                     HIPDPTEMISITNLMLNPGGLLCVVVPNDYSFFQGVLRTECGFKPWWVAPPHHINYFN
                     FDSLTNLLERCGYSVFLKEATFPIDIFLLMGCNYIGNDEVGRYCHRSRMNFEMNLWRS
                     GQNKVRRSLYQAFAQQGIGREIILYARKD"
     gene            1053..2243
                     /locus_tag="PK125_10610"
     CDS             1053..2243
                     /locus_tag="PK125_10610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB38594.1"
                     /translation="MKKENAIWMFAGGPMQEIAAREIIGMGFRLIITDRNKQCICMKY
                     ADETVELDTFDIKGNLEEAKRLKEKFHIRAAVAIAADCHETAANVGKYLQIHAIDPNI
                     SHACRFKNITRRILTEEGLLQPKFECVTNLDAACLFLKRNGGKGVVKATDNSGSRGFR
                     AIHRPEELTEQIFERAIAEGTTGIAIMEEMMCPVDNEIAEQSVETVWYNGEMYWLNWV
                     DRLFRKDFQLFEAIKTGLFDDIAWGVEIGHINPALHNYAIKKEVFDIIYRAGKAIGMD
                     RQMGGHILKADIMLTGKGPCIIELTPRCSGGWDSSGTTPLRGANFIAGIIRLALGAKL
                     DLNLWHTYFEYKNPSLYASILADVDREAIDCIGRRFAVGTGFVREKSLEKAVENLKEE
                     RYVV"
     gene            2233..2802
                     /locus_tag="PK125_10615"
     CDS             2233..2802
                     /locus_tag="PK125_10615"
                     /inference="COORDINATES: protein motif:HMM:NF016826.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016861 - intramolecular oxidoreductase
                     activity, interconverting aldoses and ketoses [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-deoxy-glucuronate isomerase"
                     /protein_id="HPB38595.1"
                     /translation="MSYRWNNEKRLMTLEVTGEENLLCFDTGSEKAEVYLEGGNIARV
                     VCEVSGITTTIDRSEAKKIIVGEGNYERAVYHYLTPNGPCPTLRLGITKHNAYGTWSS
                     LPHPFELMPEKGFEEVFFYLLEGGSERAVQVGRGIWFNGSSVEDAWFVHDHSWSTIPM
                     GYHPVVGEPGVHVSYVWAYLAKKKEWEKI"
     gene            2822..3619
                     /locus_tag="PK125_10620"
     CDS             2822..3619
                     /locus_tag="PK125_10620"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HPB38596.1"
                     /translation="MLDLFGRTAIITGAGSGIGAAVSEKLFERNCNIVMIAKSQKNVQ
                     KVAEKLVETKQASHQGTVEYYACDVRKEKELATIKDRVLMKDGKIDILVTCAAAPAAS
                     GKAEEITYEDWKSVMQTDLDGAFLSCKIFGTSMISKGYGRIVNLTSFHTIATYPERVV
                     YNVAKSGVEGLTRALAVEWGKYGITINAVAPGPIITPRTSWFLSQSPDIGKGMLSRTP
                     NRRLGEAEDVAELIAFLVSEEAKHINGQQIVIDGGWTKSAWWGSYCE"
     gene            3653..4438
                     /locus_tag="PK125_10625"
     CDS             3653..4438
                     /locus_tag="PK125_10625"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HPB38597.1"
                     /translation="MKHSLDFSLRGKVAIVTGASGDLGFAMAQALGLAGACVVLSSRN
                     RVKLEDARERIGIDKKNILTQEVDVTKPRQVKLLVRKTIESFGRLDVLVTAAGVQMRK
                     PAIDFAPREWERVLKVNLSGTFFCCQEAAKQMVSSGGGRIILISSLTAEIGLPNMAPY
                     VASRGAIRQLTKALAVEWASKGVTVNSIGPGRFRTHMTEELFASEKIRESFISLIPMG
                     RAGIASDLSGLTVFLASDHSKYLTGQSIYVDGGWLASGGNPLG"
     gene            4442..6517
                     /locus_tag="PK125_10630"
     CDS             4442..6517
                     /locus_tag="PK125_10630"
                     /inference="COORDINATES: protein motif:HMM:NF015052.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB38598.1"
                     /translation="MLSLYSIFHLNLAYSSIPESRRLEIIRRCFWPLLNLAIEDSIPI
                     AIEAPAYTLEVARMLDQDWIDVLKKAIELRKVEFIGSGYSQLIAPLVPAQVNKWNLEI
                     GVDTYRNLINKIPEVWFINEQAYSRGIVEHYSNLGVKGIIMEWNNPKTLHPEWNSEYR
                     YYPQTVIGNNNETIPVLWNDSISFQKFQRYAHNDISLKEMLQFLGGHVGSNERFFCLY
                     GNDAEIFDFRPGRFKAEPRLAQGGEWSRIRDLYHRIRDDHRFDLVLPADVLGSAPDIL
                     DLPSRIPLSLETPTHPIPVKKQPKYNVTRWQVTGRNSAWLNAQCFAIYRKIYAARKKE
                     VFSERGLKELKRHLCFLWSSDFRTHLVQERWTSLRKQILKTQEKLKHTIGDREERIQS
                     TEIPIDINGLFGAVVLGVRRRKSRKKYYAGEGIDIGERFTQIITPSVDIVLNHQRGLA
                     IESLAFPGMQPEPLIGTIKHGYFEEISLSADWYSASSVLQRPGKSQITDLEKTDPTIR
                     RGTSSKGPWIGCVGTIITEIGSIDKEYIVYQDIPQIDIKVTFHWKKVPLGSFRSGFIT
                     LLPNAFDQSSLFYATHNGGYGMEVFKMNNCVIDHGTPSSSVVTASSGLGATEGIVVVG
                     DQMKGIAVYFDQAVHAAMPMVSFRSADPSFFARLIYSLGEVDESRTQEILGAVKFVCS
                     LAGLKRSGI"
     gene            6789..9482
                     /locus_tag="PK125_10635"
     CDS             6789..9482
                     /locus_tag="PK125_10635"
                     /inference="COORDINATES: protein motif:HMM:NF014075.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF115 domain-containing protein"
                     /protein_id="HPB38599.1"
                     /translation="MDIYEKNLRALSKTHSHLVQMIEETIVDNAKVKVLRKSTDGIEI
                     SYRRPDGKTITFHDDINLSNLPPGATKLLNKKEWTKVILLLGFGLGVYPEHLLKLMDD
                     KCTLVIYEAVPEIFKVIIGLKDLSELLGSERVNLIIGDGKLDLRFIQKHHRDIARGRF
                     YVLKQSLCIETDEAAYEKFRSQFKESKMFTDVNVLTGVNRGAEWSDAFLENIPSIMRT
                     AGVARLKNLFKGYPAIIVSAGPSLEKNIHLLHKAKGRAVIIAVDVVIPTLVPAGIIPD
                     IVVVLESNRTQFLVFQDIPLLRSIPAVFSGEVVHESLSALYPGPTFFAIIHKHPASIW
                     VQRFWLDKGSHMEQFGGSVAHTAFALAQLMGADPIALVAQDLSFREKLHAGEVTELFY
                     PEEYIEQNKKRNPIVKDIFGDERYTLGQFLTFRTAFEQRISGMEGMVYNATEGGLSIK
                     GAETIRLRDFIDEHCNVPLTDFAKVIESVAISHVTCDFDAMSSDVQGDVARLKTIQKD
                     CDRIIKCVKRLKKLKERNLLSTVEAVHLVETIERLEKNVDDPIVKMVAPYRYRIENYL
                     CNNDIKDENLDVIQDFLKYYRELVDAIGSFLTKTNGLIDSLRREAEISILLSDNSLHI
                     MERYFRAGTLHLGAGMVREAATELETAANEFSNFSDPNMQKMYWPLALQVYCLLAQLY
                     IKQYRYYEAREILGVLMSFTSTKNDSQKFILNSREIEEFVNLCNQKITMWKKTKANKE
                     SLLVKAEENYGSHIESGAFYYKAGEYDRAARAYINAINEIRTLLVKNKSAYAPETIRL
                     LSAYYGLVETYAALDKQEDALIVLDSALQEVNRIRNLDLQEILEDLGVLYIDLAQRLG
                     DHSKAVSVSQSFLDILPHSFILDEKKRNLMQEGIAQISRMI"
BASE COUNT         2968 a         1715 c         2257 g         2585 t
ORIGIN      
        1 attaagaaga aacatgataa aaagatcaca aaaacttttc agtgataatg gcacacctct
       61 ggttgctcaa aaaatcatcg aggagataaa ctttggaaag aaagaaaaaa tggcaaactc
      121 atagaggttt tatacaagat caaaataaag gttttgatgt gatagaatgt gaaaagtgcg
      181 gttttaaaca tattgtgcca ataccaacca aagaagaact tcaatctgtc tatcaaaatg
      241 actattacag tatggtaaag ccgttataca tagagagata tcgggaggac cttggctggt
      301 ggaatatagt ctatgctgac cgttatgaga ccttcgagga gtgcctgcca acgaagttcc
      361 agcgtaagat gctggacgtc ggatcaggtc ccggcttcta ccttctccat ggcaaggagc
      421 gtggctggga gactctgggc atagagccat cggagaaggc tgtagctcat agccggagtt
      481 tgggactgaa cattatagaa gggtttctga cctcggataa ttttaaagac atgggcacct
      541 ttgatgtgat tcatatgagt gaagtccttg agcatattcc agacccgact gaaatgatta
      601 gcataactaa tctcatgcta aatccaggcg gtttattgtg cgtcgttgtt ccgaatgatt
      661 atagtttttt tcaaggtgta ttaaggacag aatgtggctt caagccatgg tgggttgctc
      721 ctccccatca tattaattat tttaactttg attcattaac aaacctcctg gaaagatgcg
      781 gctactcagt tttcttaaaa gaagcgacgt ttccaataga cattttttta ctgatgggat
      841 gtaactatat tggaaacgat gaagtaggca ggtattgcca tagaagtaga atgaattttg
      901 aaatgaatct atggcgttca ggccaaaata aagttagaag atccttatac caagcctttg
      961 cgcaacaagg cataggtcgt gaaattatac tttacgcaag aaaggattga gacgattgaa
     1021 acatacatgg ataaagtgag taaaaaacga gtatgaaaaa agagaatgct atttggatgt
     1081 ttgcaggtgg tcccatgcag gaaattgctg ccagggagat aattgggatg ggtttccgac
     1141 ttatcataac tgacagaaac aagcagtgta tctgcatgaa atatgcggac gagactgttg
     1201 agttagatac ttttgatatc aaaggaaacc ttgaagaagc caaaagactg aaggaaaagt
     1261 tccatattag ggctgctgta gcaatcgccg ctgattgcca cgaaactgca gcaaacgttg
     1321 gtaaatatct gcaaatccat gcaatcgatc ccaacatttc ccatgcatgt cgattcaaga
     1381 atattacccg cagaatacta acagaagagg ggctacttca acccaaattt gaatgcgtca
     1441 cgaacctgga tgcagcctgt ttatttctca aaagaaatgg tggcaagggg gttgtgaaag
     1501 caactgacaa ttcgggaagc agaggtttcc gggcaatcca cagaccggag gaattgactg
     1561 aacaaatatt cgagagggct atagctgagg gaacaacggg tattgccata atggaagaga
     1621 tgatgtgtcc tgttgataat gagattgcag aacagtctgt tgaaacggtc tggtacaatg
     1681 gagaaatgta ttggttgaat tgggtagaca gactgttccg taaagatttt caactctttg
     1741 aggctatcaa aaccggtctt tttgacgaca tagcatgggg tgtagaaatt ggtcatatca
     1801 atcctgcttt acataattat gcaattaaaa aggaagtttt tgacattatt tatagagccg
     1861 gaaaagcaat agggatggac agacaaatgg ggggacacat cttaaaggct gacattatgc
     1921 tcacaggaaa aggtccgtgt ataattgaac ttactccccg ttgttcagga ggttgggaca
     1981 gttctgggac aacaccccta agaggtgcta actttattgc tgggatcata cgattagcgt
     2041 tgggagctaa gctggacctc aatctttggc atacttattt tgaatacaaa aatccaagtc
     2101 tttatgcatc tattcttgct gacgttgata gagaggctat tgactgcatt gggagaagat
     2161 tcgcagtagg tactggattt gtacgtgaaa aatcacttga aaaggcagtt gaaaatctca
     2221 aggaggagag atatgtcgta tagatggaat aatgaaaaga gactaatgac cctggaagta
     2281 accggcgaag agaatcttct ttgttttgat actgggagtg aaaaggccga agtgtacctt
     2341 gaggggggta atattgctag ggttgtttgc gaggtttctg gtataacaac caccattgat
     2401 agaagtgagg caaaaaagat cattgtgggg gaaggcaatt atgagagagc cgtctatcat
     2461 tacttgacgc caaatggccc ttgccctaca ttgagattgg gcattacaaa acacaacgca
     2521 tacggcactt ggtctagtct tccacaccct ttcgaactga tgcccgaaaa gggtttcgaa
     2581 gaggtctttt tttatctttt agaagggggg agcgaaagag ccgttcaagt ggggcgagga
     2641 atttggttta atggttcctc tgttgaggac gcctggtttg tacacgacca cagctggagc
     2701 acaataccta tgggatatca ccctgttgtt ggagaacccg gcgtacatgt ctcttatgta
     2761 tgggcttatt tagcaaaaaa gaaagaatgg gagaagattt aacaagcaga gagggaagaa
     2821 aatgttagat ctgttcggaa ggacagccat tattaccggg gcaggaagtg gaattggagc
     2881 cgctgtatcg gaaaagcttt ttgagagaaa ctgtaatatt gttatgattg cgaaatcaca
     2941 gaaaaacgtt caaaaagttg ctgaaaaact ggtagaaaca aaacaggcat ctcaccaagg
     3001 aacagtggag tattacgcgt gcgatgtaag aaaggaaaag gagttggcaa caattaaaga
     3061 tcgagtttta atgaaagatg ggaagattga tatattggtc acgtgtgcag cagcaccagc
     3121 agcgtctgga aaagctgaag agattaccta cgaagactgg aaatcagtaa tgcaaacaga
     3181 tcttgacggt gctttcttgt catgtaaaat attcggaaca tcaatgatat ccaaaggtta
     3241 tgggagaatt gttaacttga caagtttcca cacaattgct acatatcctg aaagagttgt
     3301 ctataacgtt gccaaatctg gtgtggaggg cctgacgagg gctctggctg tagaatgggg
     3361 caaatacgga ataaccataa atgcagtcgc accaggacca atcataacac caaggacatc
     3421 atggttttta tcccaaagcc ctgatattgg aaagggtatg ctaagcagga cccccaacag
     3481 aagactggga gaggctgaag acgtggctga gctgattgca tttcttgttt ctgaggaagc
     3541 taaacacatc aatgggcagc agattgtcat tgatggcggt tggactaaaa gtgcctggtg
     3601 gggaagttat tgtgaatgat cataatatga aggggtcatt agcctagtga caatgaaaca
     3661 ttctctggat ttttcactga gaggaaaagt tgcaatcgta actggagcaa gcggtgattt
     3721 gggttttgcc atggctcagg cgcttggact ggcaggggct tgtgttgttc tctcgtcccg
     3781 taatagagtc aagcttgagg atgcgaggga gcgcattggc attgataaaa aaaatattct
     3841 tactcaagaa gtagatgtga ctaaaccaag acaagtcaaa ttattagtta gaaaaactat
     3901 tgaatcgttc ggcagattgg atgttttggt tactgctgcc ggtgttcaaa tgagaaaacc
     3961 tgcgattgat tttgcccctc gtgaatggga aagagtttta aaggttaatt tatcgggaac
     4021 ttttttctgc tgtcaggaag ccgcaaaaca gatggtgtcc tccggaggag gtaggatcat
     4081 tcttatctca tcattgactg cagaaatagg attgccaaac atggcccctt atgtggcgag
     4141 tcggggggca attcgacagc ttacaaaggc attagcggtg gaatgggcgt caaaaggagt
     4201 cacagtaaac agtattgggc caggaagatt taggacacac atgacggaag agctctttgc
     4261 aagcgaaaaa ataagagaaa gttttataag tttgattcct atgggccggg cgggcatagc
     4321 atctgatctt tcaggtctga cggtctttct tgcctcagat cattccaaat atcttacagg
     4381 tcaatctatt tatgttgatg ggggttggtt agcttccgga gggaaccctc tagggtaagt
     4441 gatgttaagc ttgtactcca tatttcatct caatcttgcc tattcatcta ttcctgaatc
     4501 cagacgcttg gagataatac ggaggtgttt ttggcccctt cttaatctag caatagagga
     4561 tagcattccg attgccattg aagcacctgc ctatacactt gaagttgccc gtatgcttga
     4621 tcaagactgg attgatgtgc tgaaaaaagc tattgagttg agaaaagtcg aatttatcgg
     4681 atcaggttac tcgcaactta ttgcaccgct tgttccagca caagtgaata aatggaatct
     4741 tgaaattggt gttgatacat acaggaattt gatcaataaa atacctgaag tatggtttat
     4801 aaatgaacag gcgtattctc ggggtattgt tgagcactat tctaatttgg gggtgaaagg
     4861 aatcatcatg gaatggaata atccaaagac tcttcatcct gaatggaata gtgaatatcg
     4921 atattatcca cagactgtaa ttggaaataa caatgaaacc attcctgttt tatggaatga
     4981 ttcaatttct tttcagaagt tccagcgtta tgcccataat gacatcagcc tgaaagagat
     5041 gcttcaattc ctgggcggtc acgttggcag caatgagagg ttcttttgtc tttatgggaa
     5101 tgatgcagaa atttttgact ttagaccggg aaggttcaag gcagaaccaa ggctggccca
     5161 gggaggggaa tggtctagaa taagagattt ataccaccgg attcgagatg accatcggtt
     5221 tgatttggtc cttcctgctg atgtcttggg ctcggcaccc gatattttgg atcttccctc
     5281 acggatcccc ctgtcgcttg aaacacccac tcatccaatc ccggtaaaga agcagccaaa
     5341 atataatgtc acccgttggc aggtaacagg caggaacagt gcatggctca acgcacagtg
     5401 ctttgcaata taccggaaga tatatgctgc aagaaaaaaa gaagtatttt ctgaaagagg
     5461 tcttaaggaa ctgaaaaggc atctatgttt cctctggtcg agtgatttta gaacccattt
     5521 agtgcaggaa cgatggacat cacttagaaa acaaatactc aaaacacagg aaaaactgaa
     5581 acataccatt ggcgatagag aggaaagaat tcagtcaact gaaattccca tcgacataaa
     5641 tggtctattc ggtgcagtgg ttttaggtgt gagaaggaga aagagtagaa agaaatacta
     5701 tgccggggaa ggtattgata tcggtgaaag atttactcaa attatcacgc cgtcagtgga
     5761 tattgttctc aaccaccaaa ggggacttgc aatcgaaagc cttgcatttc ccggtatgca
     5821 gccagaaccc ttaataggga ctataaagca tggatatttt gaggaaatat cgctcagcgc
     5881 cgattggtat tctgcaagct ctgtgttaca gaggccagga aaatcccaaa taactgacct
     5941 tgaaaaaacg gatcccacaa taaggagagg aacaagttcg aaaggcccct ggataggttg
     6001 cgttggaacg ataataacag agattggttc aattgataaa gaatatattg tctaccaaga
     6061 tattcctcag attgacatta aagtcacctt tcattggaaa aaagttcccc ttgggtcctt
     6121 caggtcagga tttatcactc ttcttcctaa cgcgtttgat cagtccagtt tgttttatgc
     6181 aacacataac ggtggttacg ggatggaagt tttcaaaatg aacaattgcg tcattgacca
     6241 tggaacccca agttcgagcg ttgtgacagc ttcaagcgga ctgggcgcca ctgaaggtat
     6301 tgttgttgtt ggtgaccaaa tgaaaggcat cgcagtctat tttgaccaag cagtgcatgc
     6361 agctatgcca atggtatcct tccgaagcgc cgatccatct ttttttgcac gcctgatata
     6421 ttctcttggt gaagtagatg agagtagaac acaggaaata ttgggggcgg taaaatttgt
     6481 ttgtagttta gctggactga aaagatccgg tatttgatca actaattaag ccgtccatat
     6541 atttcataga atcaagggga aaccttgtca atgtattgac acaaaaatat gcacataatg
     6601 ggctactttc atgtcattta tgcttgctga cggtaaattg ttagcataag tagctgtcgc
     6661 cataccattt tacgacgtgg gcaacgtgtc ttcagattaa ggtaaaacaa aaataaccgg
     6721 catatttctt gctattttat aagaagatgt tttattgagt ttcatgatac gagaaagagg
     6781 gagaaataat ggatatctat gaaaagaatc ttcgtgctct gagcaagacc cactctcact
     6841 tggttcaaat gattgaagaa acaattgtgg ataatgcaaa agttaaggtt ttacgcaaaa
     6901 gcacagatgg aatagagata tcatatcgac ggccggatgg aaagacaatt acttttcacg
     6961 acgacatcaa tctttccaac cttccgcccg gcgcaacaaa gttgttgaac aaaaaagaat
     7021 ggacaaaagt cattcttctt ctcggttttg ggctcggtgt atatccggaa cacctgttga
     7081 agctgatgga tgacaaatgc acactagtaa tttatgaggc agttccagaa atattcaagg
     7141 tcattatcgg tctaaaagat ctatcggagc ttttgggttc tgagagggtt aatctgatta
     7201 taggtgatgg caaactggat ttaagattca tacaaaaaca tcatagagac atagccagag
     7261 gaaggtttta tgtattaaaa caaagtctgt gtattgaaac ggatgaagca gcttatgaga
     7321 agtttagaag tcaattcaaa gaatcaaaga tgttcaccga tgtgaatgtc ctcacaggag
     7381 tcaatagagg tgcagaatgg agtgatgcat ttcttgagaa cataccgtca ataatgagaa
     7441 cggcgggtgt tgcaagactg aaaaatcttt tcaagggcta tcctgcgatc attgtttctg
     7501 caggcccatc tcttgagaaa aacattcacc tccttcataa agccaaggga agagcagtga
     7561 taattgccgt ggatgttgtg attcctaccc ttgtccctgc aggcatcatt cccgatatag
     7621 tagttgttct tgaatcaaac cgaacacagt ttctggtctt ccaagatatc cctttattgc
     7681 gatcaatacc agctgttttt tcaggagagg ttgtccatga atccttaagc gcactttacc
     7741 ccggccccac gttctttgcc atcattcata agcatccagc aagtatttgg gttcaaagat
     7801 tttggctgga taaaggatcc catatggagc agtttggcgg gtcagttgct cacacggcat
     7861 tcgcactggc acagttaatg ggtgctgatc ctattgctct cgttgcgcag gacctttcct
     7921 ttcgagagaa gttgcacgca ggagaagtca cagagttgtt ttatcctgaa gagtacatag
     7981 aacagaataa aaaaaggaat cccattgtaa aggatatttt tggggatgaa cgttatactt
     8041 tgggccagtt cctaaccttc aggactgcct tcgaacaaag gatttcagga atggaaggca
     8101 tggtgtacaa tgctacagaa gggggcttat ccatcaaggg ggcagagacc ataagactca
     8161 gggatttcat agatgaacac tgcaacgtgc cattaacaga ctttgctaaa gttatagaat
     8221 ctgtcgcaat atcccatgtc acctgtgatt ttgatgctat gtccagcgat gttcagggag
     8281 atgttgctcg gctcaaaaca attcaaaagg attgtgacag aattattaaa tgtgttaaga
     8341 ggcttaaaaa acttaaggag cgaaatctcc tgagtactgt cgaggcagtt catctcgtgg
     8401 aaactattga acgtcttgaa aagaatgttg atgatccaat tgtaaaaatg gttgcaccct
     8461 acaggtacag aatagagaat tatttatgca ataatgacat caaagacgaa aacctcgacg
     8521 tcattcagga ttttcttaaa tattacaggg aattggtgga tgcaatcggc agctttctta
     8581 ctaaaaccaa tggactcatt gattcattaa ggcgtgaagc agaaataagc attcttttat
     8641 ctgataactc cctccacatt atggagagat atttccgggc aggaaccctg catctgggag
     8701 cagggatggt aagggaggcg gcaactgagc ttgaaacggc ggcaaacgaa tttagtaatt
     8761 tctctgatcc aaacatgcag aagatgtatt ggcctttagc tctccaagta tactgtcttc
     8821 ttgctcagtt gtatatcaaa cagtacagat attatgaagc acgtgaaata ctgggggtat
     8881 tgatgagctt tacttccacg aaaaatgaca gccaaaaatt cattctcaat tcaagagaaa
     8941 tagaagaatt tgtaaactta tgcaaccaga aaattaccat gtggaaaaaa acaaaagcta
     9001 ataaggaatc cctccttgtc aaagctgaag aaaactatgg ctcacatata gaaagcggag
     9061 ccttttatta taaggctgga gagtatgaca gggctgcgag ggcatacata aatgctataa
     9121 atgaaattcg cacgctcctt gttaagaaca aatctgccta tgcccctgaa accatcagac
     9181 ttctaagcgc ttattatggt cttgtcgaga catatgctgc attggataaa caagaggatg
     9241 cattaattgt cttagattct gccctccagg aggtcaatag aatacgtaat cttgatttac
     9301 aagagatcct cgaggacctg ggcgttctct atattgatct tgctcaaaga ttaggtgatc
     9361 acagtaaagc cgtttcagtc agtcaaagtt ttcttgacat tttacctcat agctttatat
     9421 tggacgagaa gaaacgtaat cttatgcaag aaggtatagc tcagatatcg agaatgatat
     9481 gaatatctgc atcatattat ctaacactca tgtatccagc tcttt
//
LOCUS       DAOOMC010000109         9482 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdus sp. isolate AMR_MDS_2685
            NODE_8418_length_9482_cov_7.139069, whole genome shotgun sequence.
ACCESSION   DAOOMC010000109 DAOOMC010000000
VERSION     DAOOMC010000109.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864760
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdus sp. (wastewater metagenome)
  ORGANISM  Syntrophorhabdus sp.
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae; Syntrophorhabdus.
REFERENCE   1  (bases 1 to 9482)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9482)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,729
            Genes (coding)                    :: 2,708
            CDSs (with protein)               :: 2,708
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 7 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9482
                     /organism="Syntrophorhabdus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8418_length_9482_cov_7.139069"
                     /isolate="AMR_MDS_2685"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2699756"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1243
                     /locus_tag="PK125_10640"
     CDS             <1..1243
                     /locus_tag="PK125_10640"
                     /inference="COORDINATES: protein motif:HMM:NF036635.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB38600.1"
                     /translation="GAQGKAAEIANGVAIIAEVDQSRIQTRFSQGWVSLVAKTPEEAF
                     GVAKEYQNKKQKISIAFYGNIVDLLQHAVDKNIPIDLLSDQTSCHAAYDGGYCPQGLT
                     FEKRTELLQKDPQKFREYVDNSLRLHFSLIKTLVQKGAYFFDYGNSFMKAVFDAGVTE
                     ICKNGKDALDGFIFPSYVEDIMGPLLFDLGYGPFRWVCLSGKPEDLEKTDQAAMSCID
                     PRRRFQDRDNYAWIRDAMKNQLVVGTQARILYQDTEGRTKIALKFNEMVRKGEIGPVM
                     LGRDHHDTGGTDSPFRETANIKDGSNIMADMATQCFAGNAARGMSLVALHNGGGVGIG
                     KAINGGFGLVLDGSEKVDEVIREAIPWDVMGGVARRSWARSEQSIEVCIRYNEVNRGS
                     DCITLPYRADENMLEKLVSRT"
     gene            1359..2255
                     /gene="ftcD"
                     /locus_tag="PK125_10645"
     CDS             1359..2255
                     /gene="ftcD"
                     /locus_tag="PK125_10645"
                     /EC_number="2.1.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030409 - glutamate formimidoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030412 - formimidoyltetrahydrofolate
                     cyclodeaminase activity [Evidence IEA];
                     GO_process: GO:0019556 - histidine catabolic process to
                     glutamate and formamide [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate formimidoyltransferase"
                     /protein_id="HPB38601.1"
                     /translation="MNKIIECVPNFSEGRDAEKVEEIASSFRNSKGVKLLDIQKDGDH
                     NRSVITVVGEPGPLKEAVILAVGKAIQLIDMGIHKGQHPRMGAVDVIPFIPIRNVTME
                     DAVVLSKETAEMVWETYRLPVFLYEASASRPERKDLANIRKGQFEGMAEKVKIPEWKP
                     DFGNGGIHPTAGVVAIGARMPLVAFNVNLDTDSLEIANVIAKKVRHIGGGLRYCKAMG
                     VELKERKITQVSMNMTDYTQTPLYQALELVRIEAKRYGVNVVGSEIVGLVPMGAIVDS
                     AAYYMGLENFSAEQVLEARIME"
     gene            2278..3561
                     /gene="hutI"
                     /locus_tag="PK125_10650"
     CDS             2278..3561
                     /gene="hutI"
                     /locus_tag="PK125_10650"
                     /EC_number="3.5.2.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050480 - imidazolonepropionase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazolonepropionase"
                     /protein_id="HPB38602.1"
                     /translation="MGNELKSNGNLIIKNAAELVTCRGFAAKKGSEMSNLHVIPDGTI
                     VIEDGIIRSVGRTAEIMRNLDVSRYEVIDALGRAVLPGFVDSHTHFIFGGYRAEEFSW
                     RLQGMSYMDIMAQGGGIANTVEATRKATKEELIDLGKKRLDSMLAFGVTTVEGKSGYG
                     LDHDTEIKQLVAMGDLDAAHPIDIARTFLGAHAVPRDYRGNEDGFIEYLIQEVLPAVA
                     HKNLAEFCDIFCEKGVFSVNQSRRLLENARSFGLQLKIHADEITPMGGTELAVELGAI
                     SADHLLHASDNGIQRMAQTGTVATLLPITAFSLKEPYARARFMIDNGCAVALATDFNP
                     GSCFSESIPLLFSLATIYMGMTSEESVTALTINGAAALGREKSIGSLDVGKKGDAVIL
                     EFPSYRYLPYHIGVNCVEKVIKKGRIVVDRNKKRG"
     gene            3567..4196
                     /locus_tag="PK125_10655"
     CDS             3567..4196
                     /locus_tag="PK125_10655"
                     /inference="COORDINATES: protein motif:HMM:NF016825.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclodeaminase/cyclohydrolase family protein"
                     /protein_id="HPB38603.1"
                     /translation="MFADLPVRDFLNNVASGEPTPGGGSVSALCGALSAALAEMVARI
                     TAGKTNDEVVAKKMAEILNTASKLRKVFAENIDRDSAAYAAVMKAYKMPKETDREKKE
                     RQKAVQEALKDAAQIPLEVAEMGLRALSLAETLVKEGNQNAVTDAAVGALIARSAVVG
                     ALYNVRINLLSIKDATFRDRVKHEAEQIEHAALAKERDILTLASSVISG"
     gene            4211..5077
                     /locus_tag="PK125_10660"
     CDS             4211..5077
                     /locus_tag="PK125_10660"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HPB38604.1"
                     /translation="MRLFLEQLLNGIAMGSIYALVTLGLALVYGILRILHVAHAAVYT
                     IGAYAGLLIFNLTGSLLLAFPGAMVVCAIIGVIIERFVYFPLLKYPPFVPLIASIAIL
                     LCVEEACRLVAGPYILTFPAKLPFPDVSLGGINISSTLLAVYAITAVVLLILWYITTK
                     TELGLAMRAASQDIEISGAMGINSHFIVSITFVIGSAVAAVAGILVGIYYNQVYPTMG
                     AVPAYKTLALIVVGGLGSVPGAVVASLLLGMAETLLIGYANIPLPRDALAFIAMIGVL
                     MWRSEGLLGSSR"
     gene            5118..6002
                     /locus_tag="PK125_10665"
     CDS             5118..6002
                     /locus_tag="PK125_10665"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HPB38605.1"
                     /translation="MSAYVVTIATLMVVQAIAVCGLNVIVGYAGQISLGHAAFFGIGA
                     YSTAVLTTKLGISFWTALPLVIGISALIGLLLGLPSLRVREDFLAITTIGINFIVEAV
                     FRYVPFFGGALGIGGIPRVMFFGVRLKGIPFFLLCLGFLVLVLLICWWLTRSWASLAC
                     YAIREEEAAASTMGISPVRFKLLAFVIGTAMAGLGGSLYAHYMQFISAGDFSFPISVG
                     FLAMLVLGGMGTLWGPVVGTLILGILPELFRPLVDYRIFFYTLLLLLMIRFQPGGLLG
                     ENSFARKIITPLLKGLRK"
     gene            5999..6766
                     /locus_tag="PK125_10670"
     CDS             5999..6766
                     /locus_tag="PK125_10670"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPB38606.1"
                     /translation="MIQPLLSAKGVSKYFGGLRAVDGVSFEVFPGEILGILGPNGAGK
                     TVLFNLISGIYEPTSGEIWFSGERIDGIPPHAIAQKGIGRTFQIVKPFASLTVLQNVL
                     VARGITRYSHLTKVWGSWNSKGEIEKASEVLEKVGLSDLAERKAGLLPLGNLRRLEIA
                     RALVVGHSVLLLDESFSGLRHEEISRLEDLIRSIRKDGLTVILIEHNMRVSMGLCDRI
                     IVLDHGQKIAEGRPTEIQQDKSVIEAYLGGKGTTHAT"
     gene            6756..7466
                     /locus_tag="PK125_10675"
     CDS             6756..7466
                     /locus_tag="PK125_10675"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPB38607.1"
                     /translation="MLLEVNNIHVSYGDIQAVCGVSFHIEKGELISIIGANGAGKSTI
                     LNAVMGIFPPQQGKIIFKGQDITNIFSHQRARIGIRMVPERARVFPRLTVYENLLTGL
                     YGLRNKLPVEERISSMYSVFPILEERKNQLANTLSGGEQQQLSIARALVSNPEVLLVD
                     EVSMGLMPKLVDQVFGILEQLNKEQGLTILLVEQNAVASLAVSSRGYVLETGTCALQG
                     SAQELMDNSRVKEVYLGH"
     gene            7584..8738
                     /locus_tag="PK125_10680"
     CDS             7584..8738
                     /locus_tag="PK125_10680"
                     /inference="COORDINATES: protein motif:HMM:NF024825.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HPB38608.1"
                     /translation="MKYVRTILFCFLACVFLAGIVSAQDTIKIGLLAPLTGPAAADGL
                     SVQNSVKLAVEKVNAEGGVLGKKIELITYDDRGDAKEAVALARKLIEQDKIVAMVGGS
                     YSLPSRAMAPIFQEEKIPAVYAYAVHPDVTKAGNFNFRNGFLGMVEGKAAAYTAVKLL
                     KAKKIALLISDNDFGRTLAEGFKMYVEKYAKGAATIISVQAYPFQEKDFKAYLSKIKD
                     ENVDAIFSSGYYFQTGPVVKQAREMGIKAHIIGEEGADSPKFLEIAGKSAEGFIIVTN
                     LNRDDKRAHVQNFLKTFESRYKIQPDMVGASAYDAFMIIVDGIKRAKSVKGPEIRTAI
                     AALKDFDGLTGQIKRFTPEGEVVKDVQVQTVKNGRFRFFNVVTDPELITP"
     gene            complement(8836..9399)
                     /locus_tag="PK125_10685"
     CDS             complement(8836..9399)
                     /locus_tag="PK125_10685"
                     /inference="COORDINATES: protein motif:HMM:NF016269.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSC84-related protein"
                     /protein_id="HPB38609.1"
                     /translation="MKQRMCFMRILTIVCALAISVLFMAYHGEAKTAGEINASVNAAL
                     NRFYKQVGGAREFGAQAKALLVMPNVTKAGFLAGGQYGEGALKVGGKTQGYYNLVAGS
                     FGFTFGAQKMDIIIAFMTDDALQSFKKVKGWEVGVDGNVALIDVGGGKRLDTTTLRDP
                     IVGFVFDAKGLMVDLSLKGAKFTRVKK"
BASE COUNT         2510 a         2017 c         2440 g         2515 t
ORIGIN      
        1 cggagcccag ggaaaagcag cagaaattgc gaatggtgtt gctattattg cggaagtaga
       61 tcaatcgaga atccagacaa ggttttccca gggttgggtt agtcttgttg ccaaaacccc
      121 tgaagaggca ttcggggtgg caaaagaata tcagaataaa aagcaaaaga tatcgattgc
      181 attttatggc aatatcgttg atctcctcca gcacgcagtg gacaaaaata ttcccattga
      241 tcttctgtcc gaccagacgt cttgccatgc tgcctatgat ggggggtatt gtcctcaggg
      301 actcacgttt gaaaagcgca ctgagcttct ccagaaagac ccacagaaat tcagggaata
      361 tgtggataat tccctcaggc tacatttcag cctcatcaag acccttgtcc agaagggcgc
      421 ttattttttt gattatggaa atagtttcat gaaagcggtc tttgatgcag gcgttacaga
      481 gatttgcaaa aatggtaaag atgctctgga cggttttatc tttccgtcct acgttgaaga
      541 cattatgggc ccccttctgt ttgatctagg atatgggcct ttccgctggg tttgcttgag
      601 cggaaaacca gaagatcttg agaaaacaga tcaggcggcc atgtcatgta ttgatccccg
      661 acgaagattt caggacaggg acaattatgc atggatcaga gatgccatga agaaccagct
      721 cgtggtgggc acacaggcaa gaattcttta ccaagacact gaaggtcgca cgaagattgc
      781 cctcaagttt aacgaaatgg tcagaaaagg cgagataggc ccggttatgc tgggtcgcga
      841 tcatcacgat acaggaggga ctgactcccc ttttcgggaa acagcaaata taaaggatgg
      901 gagtaatatc atggcagata tggctaccca gtgctttgct ggcaatgctg cccgaggaat
      961 gagcctcgtt gcgctccata atggtggcgg tgtaggaatc ggcaaggcaa taaacggagg
     1021 ttttgggctg gtgttggatg gaagcgaaaa ggtagacgag gtaattcgtg aggcaattcc
     1081 ttgggatgtc atggggggtg tagcacgccg gagctgggct cgatccgagc agtcaataga
     1141 agtttgcatt cgttacaacg aggtaaacag agggtcagat tgcattaccc tgccatatag
     1201 agcagatgag aatatgctgg aaaaactggt gagccgtaca taacataccg tgttccatga
     1261 aaaacaaacc ataaaggtat cttaccatgg gcatcgatgt atttttggct gttcacggta
     1321 tatatgatat acaaacaaca gtatacaggg gttgaagtat gaacaaaata attgaatgtg
     1381 tgccaaattt cagcgaagga agagacgctg aaaaggttga agagatcgct tcatcatttc
     1441 gcaactccaa aggggtcaaa ctccttgata tccaaaaaga cggtgatcac aacagatcag
     1501 tcattacagt ggtaggtgaa ccagggccac tgaaagaggc agttattctg gcagtaggaa
     1561 aagccattca acttattgac atgggtattc ataagggaca acatccgcgg atgggcgctg
     1621 ttgatgtgat cccttttatc ccaattcgga acgtgactat ggaagatgca gttgtactct
     1681 caaaagagac agcagagatg gtatgggaaa cgtacagact ccctgttttt ctttacgaag
     1741 catcagcatc gcggcccgag agaaaggatc tggcaaatat ccgcaaggga caatttgaag
     1801 gcatggcaga aaaggtgaag attcccgagt ggaagcctga tttcggaaat ggcggaatac
     1861 accctactgc cggtgttgtt gccattggtg ccagaatgcc cctcgtagcg ttcaatgtga
     1921 accttgacac tgacagtctt gaaattgcaa atgtaattgc caaaaaggtg cggcatattg
     1981 gaggtggcct tcgttattgt aaggctatgg gggtggagct gaaagagcga aaaatcaccc
     2041 aggtttcaat gaacatgacc gattataccc aaacaccgct ttatcaggcc cttgaacttg
     2101 tccgaataga agcaaagcga tatggcgtca atgttgtggg aagtgaaatt gttggccttg
     2161 ttcccatggg agcgattgtt gactcagcag catattatat gggcctcgaa aacttcagtg
     2221 cagaacaggt gctggaagcg agaataatgg aatgaaaccg gtgacgcgta atgtgtaatg
     2281 ggtaacgagt tgaaaagcaa tgggaatctg atcattaaga atgcggcgga actggttacg
     2341 tgcagaggat ttgcggcaaa gaaaggcagc gaaatgtcca acctccacgt cattcctgac
     2401 ggtacaatcg taattgaaga cggaataatc aggagtgtag gaaggacagc agaaataatg
     2461 cggaatctcg atgtttcacg ctatgaggtg attgatgccc tgggcagggc tgtactcccg
     2521 ggctttgtgg attctcacac ccactttatt tttggtggct acagggccga ggaattttcg
     2581 tggcgcctgc aagggatgag ttacatggat attatggccc agggcggtgg tattgcaaac
     2641 acggtggaag caaccagaaa ggcaacaaaa gaggaactca tagacctggg taaaaagcgt
     2701 ctggacagta tgcttgcctt cggtgtgaca actgtggaag ggaaaagcgg atatgggctg
     2761 gatcatgata cagaaataaa acagcttgtt gctatgggcg atctcgatgc ggcccacccc
     2821 attgatatcg ctagaacctt tcttggcgca cacgctgtcc ccagggatta ccgtgggaac
     2881 gaagatgggt ttatagaata tcttattcag gaagtccttc ctgctgttgc tcataagaat
     2941 cttgctgaat tctgtgatat attttgcgag aaaggcgttt tttccgttaa ccaatcgcgg
     3001 agattgctgg aaaatgcacg ttcttttgga ttacaactga agattcatgc agacgaaatc
     3061 acaccaatgg gggggacaga gctggccgtt gaacttgggg caatctctgc agatcatctt
     3121 cttcatgcat cggataacgg gatacagaga atggcacaaa ctggaaccgt agcaacactg
     3181 cttcccataa ccgcctttag tttaaaagag ccctatgccc gtgcacgatt catgattgat
     3241 aatggatgtg ctgttgccct tgccacggat ttcaatcctg gaagttgttt ctcagaatca
     3301 attcctcttc tgttctcact tgccaccatc tatatgggaa tgacttctga agagtctgtg
     3361 actgccttga cgattaatgg cgcagctgcc cttggaagag aaaaaagcat aggtagcctt
     3421 gatgtaggga aaaaaggaga tgcagttatc cttgaatttc catcctatcg ctatttacct
     3481 tatcatatcg gcgttaattg tgtggagaaa gtgattaaga aaggtcgaat tgttgtggac
     3541 aggaacaaaa aaagaggtta aatatcatgt ttgcagactt acctgtaaga gactttttaa
     3601 acaatgttgc ctcgggggag ccaacaccag gaggtggaag tgtatcagca ctttgtgggg
     3661 ctctttctgc agcccttgct gaaatggtag cccgaataac ggcaggaaaa accaatgacg
     3721 aagttgtggc aaaaaagatg gcagaaattc taaacactgc ttcaaaatta aggaaggtat
     3781 ttgcagaaaa tattgatcgt gacagcgctg cctatgcggc agtcatgaaa gcgtataaga
     3841 tgcccaaaga gactgaccgc gagaagaagg agcgtcagaa ggctgtccag gaagctctca
     3901 aggatgcggc ccaaataccc ctcgaggttg cagaaatggg gctgagggca ttgagcctgg
     3961 ctgaaaccct ggtgaaagaa ggcaaccaga atgcggttac agatgctgct gttggagcgc
     4021 ttattgcccg gtcagcagtt gtcggtgctc tttacaacgt gagaataaat ctcctgtcca
     4081 taaaagatgc tacatttagg gacagagtga aacatgaggc agagcagata gagcatgcag
     4141 cgttggcaaa agagagggat attctcaccc tggcttcatc cgtgataagt gggtaggtcg
     4201 aggaaaatac atgcgtcttt ttcttgaaca acttttaaat ggtattgcca tgggttccat
     4261 ctatgccctt gtcacgctgg gattggccct tgtttacggt attttaagga tacttcacgt
     4321 tgcccacgcc gctgtgtaca ctatcggtgc ctatgcagga ctcctcattt ttaacttgac
     4381 aggaagcctc ctcctggcct ttccaggtgc aatggtcgtt tgcgctataa taggggttat
     4441 tatcgaacgg tttgtctact ttccactgtt gaaatatcca ccctttgttc ctctgatagc
     4501 aagtatcgct atattactct gcgtagaaga ggcatgcagg ctcgtagcag gcccctatat
     4561 cctgacattt ccagcgaaac ttcctttccc tgatgtgagt ctggggggta tcaacatttc
     4621 atctaccctg cttgcagtct atgccataac agctgttgtg cttctcatcc tgtggtatat
     4681 aacaacaaag actgagcttg gtcttgccat gcgagctgca tcacaggata tagaaatttc
     4741 aggggcgatg ggaatcaaca gtcatttcat agtgagtatc actttcgtta ttggctcggc
     4801 agtcgctgcg gttgcaggga ttcttgtggg catctattac aaccaggtat atcccaccat
     4861 gggagcagtg cctgcttaca aaacattggc cttaattgtt gttggcggtt tggggtcagt
     4921 accaggggca gtagttgcct ccctgcttct ggggatggcc gagacactcc ttatcgggta
     4981 cgccaatatc cctctaccaa gggatgcact ggcttttatt gctatgattg gagtcctcat
     5041 gtggcggtcc gagggactcc ttggttcatc acggtagatg aggtgatatg gattaatatg
     5101 gtgaatggta gttctgtatg agtgcgtatg tggtaacgat agctaccctt atggtagtcc
     5161 aggcgattgc cgtgtgcgga ctcaacgtta tagtagggta tgccggccag atttctctgg
     5221 gacatgctgc attttttggt atcggagcat acagtacagc ggtcctcaca acaaagctgg
     5281 gaatctcctt ctggacagca ctcccacttg ttattggaat cagcgcgctc ataggccttt
     5341 tactcggtct tccgagcctt cgcgtaagag aagattttct cgcgatcaca accatcggta
     5401 ttaacttcat cgtggaagct gtttttcgct atgttccatt ttttggcggt gctctcggta
     5461 ttggggggat tccccgtgtt atgttttttg gcgtgcgatt gaagggtatc cctttctttc
     5521 tcctttgcct cgggtttttg gttcttgtcc tgttgatttg ctggtggtta acacgaagct
     5581 gggcaagcct tgcctgttac gctatcaggg aagaggaagc cgcagcttcc actatgggta
     5641 tctccccagt tcgcttcaag ctgctcgcct ttgttatcgg aacggccatg gccggcctcg
     5701 gtggctctct ctatgcacac tacatgcagt tcatcagcgc aggtgatttc tcttttccca
     5761 tttcagttgg atttttagcc atgcttgtcc tcggaggcat gggaacgctc tggggcccag
     5821 ttgtgggaac gttaatcctt ggtatattgc ccgaactctt ccgtccgctt gtggattacc
     5881 ggattttttt ctacacactc cttcttctcc ttatgattcg ttttcagccc gggggattgc
     5941 tcggtgagaa cagttttgcc cggaaaatca tcacgccgct cctaaaggga ctcaggaaat
     6001 gattcagcca ctgctttcag cgaaaggggt ttcaaaatat tttggtggtt tacgggctgt
     6061 tgacggggtt tcttttgaag ttttccctgg tgagattctg ggaattctgg gaccaaatgg
     6121 agccggtaaa acagttcttt ttaatcttat ttccggtatt tatgaaccca cttcgggtga
     6181 gatatggttt tctggcgagc gcattgatgg cattcctcca catgcgattg cacagaaggg
     6241 aatcggcaga actttccaga tcgtcaagcc ttttgcatca ctgacagttt tacagaatgt
     6301 tctggtagcg cgaggaatta cacgatacag ccatcttacg aaagtctggg ggtcgtggaa
     6361 ttcaaaggga gagattgaga aggccagtga ggttcttgaa aaggttggtt tgtcagacct
     6421 ggcagagcga aaggcaggtc tattacccct gggtaacctg agacggctcg aaatagcgag
     6481 ggcactggtg gtgggacact ctgtgttgct tttggatgag tccttttccg gtctccgtca
     6541 tgaagagata tcacggcttg aagatcttat acgttctatc agaaaagatg gccttacagt
     6601 gatactcatc gaacacaata tgcgtgtatc aatgggtcta tgcgatcgca tcattgttct
     6661 ggatcatggt cagaagattg ccgaaggacg acccacggag atacaacagg ataagagtgt
     6721 aattgaggcg tatctcggag gaaaaggaac cactcatgct acttgaagtc aacaatattc
     6781 atgtcagcta tggtgatatc caggcggttt gcggtgtgag ctttcacatc gagaaggggg
     6841 agctcatttc gattattggt gccaatggag cgggtaaatc gacaatcctt aatgcggtca
     6901 tgggcatatt cccgccccaa caggggaaga ttattttcaa aggccaggat attacgaaca
     6961 tcttttctca ccagagagca agaataggga ttcgcatggt tcctgaacga gctcgtgtct
     7021 ttccacggct taccgtctat gaaaatctcc ttacaggact ttacggactc agaaataaac
     7081 ttcctgttga ggagcgcata tcatcaatgt actcggtttt tccgatcctt gaagaaagga
     7141 agaaccagct tgcaaacacc ttgtcaggtg gagaacaaca gcaactttct attgcacgtg
     7201 cccttgtctc aaatcctgag gttctcctcg tggatgaggt ttctatggga ctcatgccga
     7261 agcttgttga ccaggttttt ggaattctgg agcagcttaa caaagagcag ggcttaacta
     7321 tccttctggt agaacaaaac gcagtggcgt cgctggcagt atcatccagg ggttatgtgc
     7381 ttgaaacagg cacgtgtgca cttcagggca gcgcgcagga attgatggac aattcgaggg
     7441 tgaaggaggt atatctgggg cattagataa aaagatgctt atttaaccgc aggtgtgccc
     7501 ggatgatcac agataaaaac cgtctgtggc aaaaatgtag tatgattttt tcaatttaga
     7561 gattctaaaa aaggaggtag agtatgaagt atgttagaac aatcctgttt tgttttttgg
     7621 cgtgtgtttt tctggcgggt atagtatcag cacaggacac gatcaaaatt ggccttctgg
     7681 ccccccttac aggtccagct gcggctgatg gccttagcgt tcagaactca gtgaagctcg
     7741 cagtggaaaa agtgaatgcc gagggaggag tgcttggcaa gaaaattgaa ctcataacct
     7801 acgacgaccg tggagatgcc aaagaggctg tagcacttgc ccgtaagctg attgagcagg
     7861 acaagatcgt tgccatggtg ggcgggtcat acagcctgcc aagcagggct atggctccca
     7921 tttttcagga agagaagatt ccggcagttt acgcctatgc tgtccacccc gatgtgacca
     7981 aggccggtaa tttcaacttt cggaacggtt ttcttggaat ggtcgaaggc aaggcagctg
     8041 catatacagc agtaaaactc ctgaaggcaa aaaagatcgc tctccttatc agtgacaatg
     8101 attttgggcg gaccctggcc gaaggtttta agatgtacgt tgagaaatat gccaaaggtg
     8161 ccgcaacaat catttctgta caagcttatc ctttccagga aaaggatttc aaagcttatc
     8221 tgtcgaagat aaaggatgag aacgtggacg ctattttttc cagtggctac tatttccaga
     8281 ccggacctgt ggtgaaacag gccagagaga tgggtattaa ggcgcatata atcggtgaag
     8341 agggggcgga ttcacccaag tttctcgaaa ttgcaggcaa gtcagccgaa ggtttcatta
     8401 ttgtcaccaa cctgaaccga gatgacaaaa gagcccatgt ccagaacttt ttgaagacct
     8461 ttgaatcaag atataaaatc cagcctgata tggtgggcgc ctcggcatat gatgccttca
     8521 tgatcattgt ggatggcatt aaacgagcca aaagtgtgaa gggcccagag atcaggactg
     8581 ccatagcagc gctcaaggat tttgacggtc tcacaggaca gattaaacga tttacacctg
     8641 agggagaagt ggtaaaagat gtccaggtcc agactgtaaa aaatggcagg ttccggtttt
     8701 ttaatgttgt caccgatccg gaactgatca caccctgata atgatttttt tatgattcaa
     8761 aaggggcagc tctattcctt cccatagctg cccctttttt atgatcccaa ggaacttatt
     8821 caggtctgat gttagctatt tcttaaccct tgtaaactta gctcccttta gagaaaggtc
     8881 caccatcagc ccttttgcat cgaagacaaa acctactatt gggtccctga gggttgttgt
     8941 gtcgagacgt tttccgcctc ccacatcaat aagcgctaca ttaccatcaa caccgacttc
     9001 ccagcctttt acctttttga agctctgtag cgcatcgtcg gtcatgaagg ctataataat
     9061 atccattttc tgagcgccga atgtgaaacc gaaagacccg gcaacaaggt tatagtagcc
     9121 ctgagtcttt cccccaacct tcaaagctcc ctcgccatac tgtcctcctg cgaggaaacc
     9181 cgcttttgtt acattcggca ttaccagtaa tgccttggcc tgagccccaa actccctggc
     9241 tccaccaacc tgtttataga agcggttcag tgcagcattt acgcttgcat tgatttcacc
     9301 tgcagttttg gcttctccgt ggtaggccat aaagagaact gagattgcaa gtgcacacac
     9361 tatggttaaa atcctcataa aacacattct ttgtttcatg aacgtacccc ctaatggatg
     9421 atcacccttt tatagggatt atggatagta aattgtaagc atgtgaaggc tgttgagtca
     9481 ag
//
LOCUS       DAOOMC010000110         9367 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdus sp. isolate AMR_MDS_2685
            NODE_8564_length_9367_cov_7.215206, whole genome shotgun sequence.
ACCESSION   DAOOMC010000110 DAOOMC010000000
VERSION     DAOOMC010000110.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864760
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdus sp. (wastewater metagenome)
  ORGANISM  Syntrophorhabdus sp.
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae; Syntrophorhabdus.
REFERENCE   1  (bases 1 to 9367)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9367)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,729
            Genes (coding)                    :: 2,708
            CDSs (with protein)               :: 2,708
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 7 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9367
                     /organism="Syntrophorhabdus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8564_length_9367_cov_7.215206"
                     /isolate="AMR_MDS_2685"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2699756"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(787..1002)
                     /locus_tag="PK125_10690"
     CDS             complement(787..1002)
                     /locus_tag="PK125_10690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB38610.1"
                     /translation="MEHRILHAEVLQSRSSTDFIRLFESCAMTVLQEVLVNALSFNVR
                     EDDNGLGRLLCHFFKAVSRCEVILHYL"
     gene            complement(1386..2054)
                     /locus_tag="PK125_10695"
     CDS             complement(1386..2054)
                     /locus_tag="PK125_10695"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HPB38611.1"
                     /translation="MNTIKDEIYETRKKFFNDHAEKWRDMWYKDQTTGRYDKHIKDFE
                     RLFSLLPLKPGDCVLDVGCGTGILVPHILERITATGTLYELDFADKMIEANKILNKED
                     NIRFIISDVESTPLNDASCDVVICFSCFPHFHDKEKAVKTLSRILKKQGAFIVSHFES
                     SEGINKHHESCHAVMHDHLPDKASMLVLLQAESLDVDYFIDEPGFYYISAKKVARPEH
                     GEHD"
     gene            complement(2035..2922)
                     /locus_tag="PK125_10700"
     CDS             complement(2035..2922)
                     /locus_tag="PK125_10700"
                     /inference="COORDINATES: protein motif:HMM:NF013143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HPB38612.1"
                     /translation="MTEEDLSLMVDQIMDVLTYPPIQKAALACILCGASCSLLSVFVV
                     LMKMPLIGVSMSHAAFAGAVMGMLLGFNPFLCGFIMCLLAAGVLGPLSDRTDIAPENV
                     LGIMFSFLMGVAFLGMGILTRTKAGALNLMWGSLLSLSDSDIAVLTVITAILLLFIVM
                     FFKEIRYVLFNRKLALAGGVPERFIYYALLFLTGAVISSNLATVGGLLIFALLVQPGA
                     TALQLTYNLRHFFLISVAAGIGSCVSGLIVSYVFNLPSGASVVLMATTIFAAAYVFSP
                     KRHRRKKAKECISYEYDKR"
     gene            complement(2891..3667)
                     /locus_tag="PK125_10705"
     CDS             complement(2891..3667)
                     /locus_tag="PK125_10705"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HPB38613.1"
                     /translation="MNEHIIELSGVTVRKAKGVLLDGISLNVARNEFVGIIGSNGAGK
                     TTLLNVIAGFERFEGELSLFGKPERWKRSRETRVRVGYIPQLFQIDPAFPILVSEAVM
                     TGAIGRVGLFRSPGRKEKEKAMGLMEMMRVAHLLDRPLGQLSGGERQKVSLARVILQQ
                     PDILLMDEPTANLDIAVQKEVLNLINEIHQKENLTVLFVTHDFNMIPAGMKRVVLLNH
                     GKIVFDGDIDVALSGDMLSTLFQYPLETFERDGRRFISYG"
     gene            complement(3681..4400)
                     /locus_tag="PK125_10710"
     CDS             complement(3681..4400)
                     /locus_tag="PK125_10710"
                     /inference="COORDINATES: protein motif:HMM:NF013465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HPB38614.1"
                     /translation="MTSDTILSGMVAALLPPNRYSVEAMLPPGQCPGHYDVKLSDIEK
                     MKRAALTLSFKGIPFMNKTVLEVRAQLLVDAEDRNWMVPDSYIYGLSLLADGLSKHFP
                     EDRDEIMRRKNDAIREVRAEANTLTEKINKRGGIGKPVIASSMQKQTLHWMGFHVVGE
                     YGRPEAISAREVVQLSKIGKDQRVIAVVDNLQSGPDTGKGIAEALGVPHIILTNFPSE
                     EGYLVTLGENVNKMLSAVVQK"
     gene            complement(4623..6704)
                     /locus_tag="PK125_10715"
     CDS             complement(4623..6704)
                     /locus_tag="PK125_10715"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HPB38615.1"
                     /translation="MKAASKFVIGLLLFAFVTVTWALAEEPQKGKKEEKAILEEITVT
                     EAPYTNPVTPVDTRYGTRYNLVTEEQIKEQNALDFQSTLRDVPGVMFQSKNIVGSQTS
                     HSLYIRGRGSSHPSADFSILFDGAPRFGALFGQVLGDGIPVSAIERIEIYKSPQPSQF
                     GSGYASINILPKYLRKEGQEAVFNASAGSYGTVDEDFSVGVKRGPFDIYMSQSWVRTD
                     GHRNHSGADQESYYANAGYQISKEWNIRFLVNYVDSKTEAPMPDAIPNAKNGVSWPGA
                     ERYDTETFFTTLTLNHEYDRFSGYLKSYYNNTYFDLLQELRNGVRYGGNTGGLKSKQE
                     LGLYGVRGREKLHLWPGGEILVGADLDMTKLKNTQKTYSGLATPGINGGLAKRVWDFP
                     DTTLFSPYLAVSQLVGRSDNFHIIPSAGFRHFVHDEFKDKSSSQAGLVIGYRHTDLSL
                     NYSRGVNYPSPVALMNMVLESSPVTKPEQYWKKLKPEVVDHYEIGLTHIWPKAASLGA
                     TAFVDKGKDRYQAYMFGPIPIQFNDPIGKYEIRGLELTGTLSLIKNLELFAATTWLDA
                     EATGSNRIKRDHLPYTPSFQFQAGANWTFLENFRLFMDMQHLRGLYQGTVSRAGGFNI
                     GDPGPINKLDDITLFNARLSYRFNYKALRLNDSEVFVAVNNIFDQEYEYAKGYPMPGT
                     TFYAGISLRFN"
     gene            complement(6953..7702)
                     /locus_tag="PK125_10720"
     CDS             complement(6953..7702)
                     /locus_tag="PK125_10720"
                     /inference="COORDINATES: protein motif:HMM:NF015506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HPB38616.1"
                     /translation="MKENHLGALVSVTVHSCVIPLLVAASLSVQIAPVKVVEVDFSLI
                     KNQQRMNSTLDSEKKIIEAKPVLLKGKKSSRGIFEMDHPVQESPQTVPVKEGLDASPV
                     PTIVTASDDQGEMVVHGVPATYADSSGTGKFLQPHGSLSGSLKGSGQGFGQGGESLTV
                     GSKDYNYIRDAVIKNVKYPEQARRLGLEGKVLLSFIVLENGTTKEIKVMQSSGHALLD
                     KSAKEAVALTRISRRTPYRVAVSLPITFRLQ"
     gene            complement(7699..8082)
                     /locus_tag="PK125_10725"
     CDS             complement(7699..8082)
                     /locus_tag="PK125_10725"
                     /inference="COORDINATES: protein motif:HMM:NF014525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HPB38617.1"
                     /translation="MDEKEFDYINMIPFIDVMLVLLTIVLMTSTFVVTGIIPVELPRV
                     AGKHEAATVTHVVVIDKQGALSYQGKAITLLDLKNIVIDVPKETPFLIKADKDIHLQS
                     FVEVLDLIKTAGFKKVSLQTEWVGQ"
     gene            complement(8075..8500)
                     /gene="exbB"
                     /locus_tag="PK125_10730"
     CDS             complement(8075..8500)
                     /gene="exbB"
                     /locus_tag="PK125_10730"
                     /inference="COORDINATES: protein motif:HMM:TIGR02805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-system energizer ExbB"
                     /protein_id="HPB38618.1"
                     /translation="MAWLVYLVDYGIVGLLILLSIISLAIFIERWVVLKKIDPAHYKG
                     SRRILEIKITQKLGVLASIGSNAPYIGLLGTVLGIMLTFYTVGVEGYVDATRIMVGLA
                     LALKATAIGLVVAIPSIVFYNILLRRVKVILLEWDDLHG"
     gene            complement(8728..9144)
                     /gene="nikR"
                     /locus_tag="PK125_10735"
     CDS             complement(8728..9144)
                     /gene="nikR"
                     /locus_tag="PK125_10735"
                     /inference="COORDINATES: protein motif:HMM:NF003381.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0010045 - response to nickel cation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-responsive transcriptional regulator
                     NikR"
                     /protein_id="HPB38619.1"
                     /translation="MAELFRFGVSLDKALLDKFDRYIRERNYSNRSEAFRDLIRQELI
                     KKEWNEGDDVAGAITLIYDHHRRDLLNKITDLQHDFQKVIISTQHIHLDHDNCLEIVA
                     VRGKPEEIIRLTDMLKSIKGVKHGTLSMSSTGKDIE"
BASE COUNT         2530 a         2243 c         1936 g         2658 t
ORIGIN      
        1 atcatattgt tatttatcgg cagctgtcaa ggaaaatctt aaaaaatttt aataaaataa
       61 attgtttttt tctatgaaaa aatcttgcat ctttatcatt tcaaatgggc aaccctgctt
      121 aactgagatt ttgtctgctg ataacaggtc aagtgatcgc tttgactttc tcaaaaaccc
      181 tatatattcc ggcccttgcc gttactgctt ggatgaggct gttgaggcat tcaagggtgc
      241 agttatcgat ttttcaggtg taacagtaac tggtacctgc ccatcatcgc ttcattattt
      301 ctgcagtctc cataattgtc gtatcaacta atgcatctca gatgtaccac ttttttgagc
      361 atcgcctgaa aaccttgccg tgttgatact ctatagatga agtttaaaag ttatcagaat
      421 ataaggacag ttcttataat ggagcggtcc aggattcaca gtaggtgtac aataccatct
      481 gattcatcgc aagattccct gcttttcatt catagatact ttgcactgag gagcatctcc
      541 tgatctccct cttacacacc tcacctgttg cggcgttgag aatatttatg atgtggaatt
      601 tatcgaagat aatgactgca ccaatggtat attccggtga accgttgctt aagcgaggcc
      661 atccttgaaa tttgcttggt tgatgcatcc ctctcttcat catcgcatat agtccacttc
      721 tcttccggat caacaacgag cgttgcataa tcatggccta ttattgcaga cgtctaatcg
      781 ctctgattac aggtaatgaa gtataacctc acagcggctg actgctttaa agaaatggca
      841 aagcagcctc ccgagcccgt tatcatcttc tctgacattg aaggaaaggg cattcaccag
      901 cacctcctgc aatacggtca tagcgcacga ttcaaagagg cggataaagt cggtactgct
      961 ccgtgactgc agcacctcag cgtgaagaat cctgtgttcc acgcattcga ctcttgattc
     1021 ccaaggggga agacaactgg cataccggaa gaggttgaga tgtttccatg ggtatcccac
     1081 cgttatatca gaccatggca agaagatcgg tgaaggaggc tggaatacat ggtatggcgg
     1141 tcctaacgga taaaaagtga atgttgaggg tatggaggaa gctgggattt ctctccatcg
     1201 agatggcaga cagaatgagc agggtaatgc tgagcctgaa ataagataaa tggcctgctc
     1261 gggtgtacac gtttataacc aaccacgggt gaggagctga tcaagttgaa ggttgctcct
     1321 cacagactcc atgagtccca accataagac gtaatgaaaa gattatgacc atgccaataa
     1381 tgaagtcaat catgctctcc gtgttccggt ctggctactt ttttcgcact aatataataa
     1441 aaaccaggct cgtcaatgaa gtaatcgaca tccaggctct cggcctgcaa taaaacaagc
     1501 attgacgctt tgtcaggtaa atgatcatgc attacagcat ggcatgattc gtgatgtttg
     1561 ttaatgcctt ctgacgattc aaaatgtgat acgatgaacg cgccctgctt tttgagaatg
     1621 cgcgatagcg tcttcacggc tttttcttta tcgtgaaaat gcgggaaaca cgaaaaacag
     1681 ataacaacat cgcatgaggc gtcattgagt ggtgtgctct ccacatccga gatgataaac
     1741 ctgatgttgt cctccttatt gaggatctta ttagcttcta tcatcttatc cgcaaagtcc
     1801 aactcataga gggttcctgt cgctgtaata cgctccagaa tatgcggaac cagtattcct
     1861 gttccgcatc cgacatccaa cacgcagtct cctggtttta gtggcagaag agaaaacagg
     1921 cgctcaaagt cttttatgtg cttgtcgtag cgtcctgtag tctggtcttt gtaccacata
     1981 tcacgccact tttcagcgtg gtcattaaag aatttttttc gtgtttcata gatttcatct
     2041 tttatcgtat tcataggata tacactcttt cgcctttttc ctcctgtgcc tttttggtga
     2101 gaaaacgtat gcagcagcga agatagttgt cgccatgagc accaccgatg caccggaggg
     2161 aagattaaaa acataggata ctataaggcc cgaaacgcag gagccaattc ccgcagctac
     2221 ggaaataaga aaaaagtgtc ttagattgta cgtcaattga agcgccgtgg ctccaggttg
     2281 tacaagtaac gcaaaaatga gcagaccacc aactgtagca agatttgacg aaatcacagc
     2341 tccggttaga aacaaaagcg cataataaat gaagcgctcc ggaactccgc ctgccaatgc
     2401 aagctttctg ttaaatagga cataacggat ttccttgaaa aacatcacaa taaaaagcaa
     2461 aaggatcgct gtgatgactg tcaatacggc aatatccgag tcggacagcg ataacagact
     2521 cccccacatc agattcagag cgccggcctt tgtccttgtg agaattccca taccaagaaa
     2581 tgcaactccc ataagaaaag aaaacataat gccaagcaca ttttcagggg ctatatccgt
     2641 gcgatctgaa agtggaccga gtactcctgc cgcaagaaga cacataataa aaccacatag
     2701 gaatggatta aacccaagca acatacccat tacagcacct gcaaaagcgg cgtgtgacat
     2761 cgagacacct attaacggca ttttcatcaa aacaacaaat accgacaaca gcgaacaact
     2821 tgcgccgcac aggatacatg caagagcagc tttctgaatg ggtgggtacg ttaaaacatc
     2881 cattatctgg tcaaccataa gagataaatc ttcttccgtc acgctcaaat gtctccagtg
     2941 gatactggaa caaagtgctg agcatatccc cggacagagc aacatcaatg tcaccgtcga
     3001 aaacaatttt cccatgattc agcagaacaa cacgtttcat gcctgccgga atcatattga
     3061 agtcgtgtgt gacaaaaaga acagtcaagt tttctttttg gtgaatttca ttgataagat
     3121 tcaatacttc tttctggaca gcaatatcaa gattcgcagt tggttcatcc ataaggagga
     3181 tatcgggctg ctgaagaatc acgcgcgcta atgacacctt ctgccgttcg cctccagaga
     3241 gctgtccaag tggtctatcc agaagatgag ccacacgcat catttccata agacccatcg
     3301 ccttttcttt ctctttcctc ccgggagagc ggaatagtcc gacccgtccg attgcgcctg
     3361 tcattacagc ctctgacaca agaattggaa atgccgggtc tatttggaat aattgtggaa
     3421 tgtaaccgac gcgaaccctt gtttcacgcg atcgcttcca cctttcgggt tttccgaaaa
     3481 gactgagttc cccctcaaac ctttcaaatc cagctataac atttaagagt gtcgtcttac
     3541 cggccccatt ggacccaatg atccctacaa attcattgcg cgctacattc aggctgattc
     3601 catccagcaa caccccttta gcttttcgga ctgttacccc gctcagttca ataatatgct
     3661 cattcataat agattcagta tcatttctgg accacagcag acaacatttt gttaacattc
     3721 tctcccagtg tgacaagata gccttcttcc gatggaaaat tcgtcaggat aatatgtggt
     3781 acccctagtg cttctgcgat tccttttcct gtgtcgggcc ctgactgaag attgtccacc
     3841 acagctatca ctcgttgatc cttccctatt tttgaaagct gcacgacttc tcttgccgat
     3901 attgcctccg gcctgccgta ttctcccaca acatggaaac ccatccaatg gagagtttgc
     3961 ttctgcatgg aagaagcaat gactggcttt ccaatgcccc ctctcttatt aatcttttca
     4021 gtgagggtgt ttgcttccgc tctaacttct cggatcgcat cgttttttct cctcattatc
     4081 tcatcgcgat cttcgggaaa atgttttgag agcccatcgg caagcaggct caacccatat
     4141 atgtatgaat ccggcaccat ccagttgcgg tcttcagcat caacaagaag ttgcgctctg
     4201 acctccaaaa cagttttgtt catgaaaggg atgcctttga aggaaagggt tagtgcagcc
     4261 ctcttcattt tctctatgtc cgatagttta acgtcataat ggcctggaca ctgccccggc
     4321 ggaagcattg cttcaactga atagcgattc ggtggcagaa gagcagcgac catcccagac
     4381 aggatagtat ccgaagtcac gatatacctc atggtctgcg cgttcccgtc ttttgagctc
     4441 ttcgaacatc cagcgaaaaa aaatacaaat gctattacca taataaccag aaatgtcgtt
     4501 gtttttgtca gcactgacag atccttttcc agactcaccg ttccggtata tattcattca
     4561 tcaaggggaa accctttatg tggttgggat aacgcaatcc cctgtaatag aaagaaccaa
     4621 actcaattaa acctcagact gattccggca taaaaagtcg taccgggcat agggtagcct
     4681 ttggcatatt catactcttg gtcaaaaata ttgtttactg ctacaaaaac ttccgaatca
     4741 ttcagccgga gcgccttata attgaagcga tagctcaggc gggcgttaaa cagtgtaata
     4801 tcatcgagct tatttatggg tcccgggtca cctatgttaa aaccaccggc tcgtgagacc
     4861 gtgccctgat aaaggcccct gaggtgctgc atatccatga aaagccggaa attttccagg
     4921 aacgtccagt tggcacctgc ctgaaactga aaactgggag tgtagggtaa atggtctcgt
     4981 ttaattctgt tactgccagt ggcttcggca tccaaccacg tggtagcggc gaaaagttca
     5041 aggtttttga tgagcgataa agtgcctgtg agctcaagtc cgcgaatctc gtatttgcct
     5101 atcgggtcgt taaactgaat tgggattggt ccaaacatgt aagcctgata acgatccttc
     5161 cccttgtcta cgaaggctgt tgcccccaga gaggctgcct ttggccagat atgggttaaa
     5221 ccaatttcat aatgatccac cacttccggc tttaattttt tccagtactg ctctggtttt
     5281 gtcactggcg agctttcgag aaccatgttc atcaatgcga ctggagaggg atagttgacg
     5341 cctcgggaat aattgaggct aaggtccgta tgtctgtatc ctattaccag accggcctgg
     5401 gatgatgatt tatctttgaa ctcgtcatga acgaaatgtc gaaaacctgc agaggggatt
     5461 atatggaaat tatcggaacg gccgaccagc tgacttactg ccagataagg tgaaaagagt
     5521 gttgtatctg ggaaatccca aacccttttt gccagacctc cattaattcc cggagtcgcc
     5581 aacccactgt atgttttttg agtgtttttt agttttgtca tatccaaatc tgctccgacc
     5641 aaaatttccc cacccggcca aagatggagt ttttccctgc ctcggactcc gtaaagcccg
     5701 agttcttgtt tggacttcaa cccgccagta ttacccccgt accgaacgcc atttctcagt
     5761 tcctgtaaga ggtcaaaata agtattatta taataggatt tgaggtagcc gctgaaacga
     5821 tcatattcgt gattaagtgt caaagtggta aaaaaggttt cagtatcata acgctctgct
     5881 ccaggccaac ttaccccatt ctttgcgttt ggtatggcgt caggcatagg cgcctcggtt
     5941 ttactgtcca cgtagttcac caggaatctg atattccatt ctttgctaat ttgatagcct
     6001 gcattggcat aatagctttc ctggtcagca cctgaatggt tacgatgacc gtctgttctc
     6061 acccagctct gagacatgta aatatcgaac ggtcctcttt tgacgcccac agaaaaatct
     6121 tcatcaacag taccgtaact tccggcactt gcattgaaca cagcctcctg accctctttc
     6181 ctaaggtatt ttggcaggat attgatcgat gcatatccac tgccgaactg ggatggctgc
     6241 ggactcttgt agatctcaat cctctcgatt gcggaaacgg ggataccatc gcccagaacc
     6301 tgtccaaaca atgcgccaaa gcggggagcg ccgtcgaata ggatggaaaa atctgcgctt
     6361 ggatggctcg aacctcggcc tcgaatgtac aggctatgac tcgtctggct tcctacaata
     6421 ttcttgctct ggaacataac ccccggaaca tcacgcaggg tggattggaa gtcaagggca
     6481 ttctgttctt tgatctgttc ttcagtcacc agattataac gagtaccata gcgggtatca
     6541 acaggagtaa cagggttggt gtacggggct tctgttacgg taatctcttc caatattgcc
     6601 ttttcttcct tttttccttt ctgtggttct tctgctaatg cccatgttac tgtgacaaag
     6661 gcgaagagaa gaaggcctat tacaaatttt gatgctgcct tcattatcct ttcctccttt
     6721 taccaccacc tttgctctaa tttagaaaaa gtatggggta tattgttcgt tactttctta
     6781 caaagggtga ggcagttcct gctaacgtta gccttacacc tgcctaaccc tcgttacagt
     6841 ctattttcac tgaaacctct ctatcatttt ctaaccattt ttcaacacca accatagctt
     6901 ggcaccagaa ggaataatcg atatataaat tctacattct gtcatggttt ggtcattgaa
     6961 gcctgaatgt tataggaaga ctaactgcaa ccctgtatgg cgtcctcctg gaaatacgtg
     7021 tcaatgccac agcctccttt gcacttttat ccaatagcgc gtgaccggaa ctctgcatca
     7081 ccttaatctc tttcgtagta ccattttcca acacaataaa agaaagaagc acctttcctt
     7141 ctaatccgag tctccttgcc tgttcaggat atttgacgtt cttgattacc gcatcgcgga
     7201 tataattgta gtctttgctt cccactgtca agctttcacc gccttgtcca aacccttggc
     7261 cgctcccttt caaactgcct gataggctgc cgtgcggttg aaggaacttg cctgtccctg
     7321 aagagtctgc ataagttgcc gggacaccat ggacaaccat ttcgccttgg tcatctgacg
     7381 ctgtgacaat cgtcggaaca ggagatgcat caagtccttc ttttaccggt acggtttgtg
     7441 gactctcctg aacagggtga tccatttcaa atatgcctct tgatgatttt ttacctttta
     7501 agagaactgg ttttgcctca attatcttct tttcactatc cagcgtcgaa ttcatcctct
     7561 gctgattttt tatcagggaa aaatcgactt ctaccacttt aaccggcgca atctgaactg
     7621 ataatgatgc ggccacgagt agtggtataa cgcatgaatg cacagtaaca gagacaagtg
     7681 ctcctaagtg attttctttc attggccaac ccactcggtc tggagactta cttttttgaa
     7741 gccagctgtc ttgataaggt caaggacttc gacaaaactc tgaaggtgga tatctttatc
     7801 tgcctttatg aggaatggcg tttcttttgg cacatcaatg acaatgttct taagatcaag
     7861 aagggtgata gctttgccct ggtaagagag agcgccctgt ttatcaatca ccacaacgtg
     7921 cgtcaccgtg gcagcttcgt gttttccagc cacccgcggc agctcaacag gtatgatacc
     7981 ggttaccaca aaggtgctcg tcatgagtac tatagtaaga agcacgagca tcacgtcgat
     8041 gaaagggatc atgttgatat aatcgaattc tttctcatcc atgcaggtca tcccactcca
     8101 gaaggatcac tttaacacga cgcaaaagta tgttgtaaaa gacgatggat ggtatggcta
     8161 ccaccaggcc tatggcggtt gccttgagag caagggcaag cccaaccatg atccgtgtgg
     8221 catcgacgta tccctcaaca cctacagtgt aaaacgtcaa catgatgccc agcacagttc
     8281 ccagcagacc aatgtaaggt gcgttactcc caatggaggc aagcactccc agtttttgcg
     8341 ttatctttat ttccaggatg cgtctgctcc ctttgtaatg agcaggatca atcttcttga
     8401 gtacaaccca ccgttcgata aaaattgcca aagaaattat ggacaggaga atcagcaatc
     8461 caacgatacc gtaatctacc aagtaaacaa gccaagccat tacaaatccc cgaccatcat
     8521 ctgaactacg accacataat gctgtgcata tgttcgttgc aacaactttt tagttgacct
     8581 atgaaattta ctcctcatca tcaaatcatc ctttagagca atggttattc ttacagtctt
     8641 cttcctcact cagagtttat cctttgaacc agcgttaatg ctacaaattt attattcgtg
     8701 acacaattca gcaaaaaata atactcctta ctcaatatcc ttgcctgtac ttgacatgct
     8761 caacgttccg tgttttaccc ctttgattga tttaagcatg tcagtcagtc ttattatttc
     8821 ttcaggtttt cctctaacag caacaatttc gaggcagttg tcatggtcca gatgaatgtg
     8881 ctgcgttgaa ataataacct tctggaaatc atgctgaagg tcggttattt tgttcagtaa
     8941 atctcgtcta tggtggtcgt aaattagcgt gatagcccct gctacatcgt caccctcatt
     9001 ccattctttt tttataagtt cctgcctgat tagatctcta aatgcttcgg aacgatttga
     9061 atagttgcgc tcccgtatgt agcgatcaaa tttatcaagg agagctttgt cgagagaaac
     9121 cccgaaacgg aatagttctg ccattacctt ccacgatagg atatttgaag agtatgtagc
     9181 acattgaatc gattgtgtaa agaacatttt gtagattgtc caatccgttt ttcattgcat
     9241 atccatctcg tttgaaggaa aataggacaa ttatgactac atttccgctg aaaattttga
     9301 acaatagaaa catgattttc cttctggcac tcatccttgg gtttctctgg gatacaggag
     9361 cacatta
//
LOCUS       DAOOMC010000111         9240 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdus sp. isolate AMR_MDS_2685
            NODE_8727_length_9240_cov_6.343386, whole genome shotgun sequence.
ACCESSION   DAOOMC010000111 DAOOMC010000000
VERSION     DAOOMC010000111.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864760
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdus sp. (wastewater metagenome)
  ORGANISM  Syntrophorhabdus sp.
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae; Syntrophorhabdus.
REFERENCE   1  (bases 1 to 9240)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9240)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,729
            Genes (coding)                    :: 2,708
            CDSs (with protein)               :: 2,708
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 7 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9240
                     /organism="Syntrophorhabdus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8727_length_9240_cov_6.343386"
                     /isolate="AMR_MDS_2685"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2699756"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(135..1196)
                     /locus_tag="PK125_10740"
     CDS             complement(135..1196)
                     /locus_tag="PK125_10740"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: protein motif:HMM:NF009897.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HPB38620.1"
                     /translation="MKIEITKIDKSRLQSVDFDRLQFGEILSDHMVLMDYDNGRWLSP
                     KIVPYGPMEIYPALCTFHYGQAIFEGLKAFYGHGGEIKVFRPDKYHARMLRSSERLCI
                     PDIGFETFISSLKELLLLDRGWVPRKKGYSLYIRPFIIAMDNFLGVRVSEKYRFMIIT
                     SPVGAYYKEGINPVKLITSGEFVRAVRGGLGMAKTPANYAASLYPGEEAKRKGFTQVL
                     WLDGIERKYIEEVGTMNICFVIDEELVTPPLEGSVLDGITRQTVIHLWKDRGKKVSER
                     RISIDEVIETAKSGAMQECFGTGTAAVISPVGEIRHGDVNVVINSGKIGPAAQSLYDE
                     ITGIQYGERPDRFGWCFKI"
     gene            1322..2080
                     /locus_tag="PK125_10745"
     CDS             1322..2080
                     /locus_tag="PK125_10745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012096464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HPB38621.1"
                     /translation="MVISISNQKGGVGKTTTAVNLSASIAVAERRTLLVDIDPQCNAT
                     SGFGISYNRLDASLYHVLIGEKSIKDVIRKTMIPFLDIIPSHPDLIGCEIELLEAEER
                     EFILRKALDEIITDYDFIFIDCPPSLGLLTINALAASDYVIIPLQCEYFALEGLALLQ
                     RTISIIKKRLNPKLDTLGILLTMFDRRNNLSFRVWEEVNKCFVDGVFKTVIPRNVKLS
                     ESPSYGKPVILYDISSRGAESYLELASEILEKRG"
     gene            2081..2899
                     /locus_tag="PK125_10750"
     CDS             2081..2899
                     /locus_tag="PK125_10750"
                     /inference="COORDINATES: protein motif:HMM:TIGR00180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HPB38622.1"
                     /translation="MLKKDPLGRGLSAILQDVEERGSSRLIPIEQIIPNPEQPRIAMR
                     EETILELATSIQEKGLLQPILLRRKDGKYEIIAGERRYRASLKAGLKEVPAIIKDVDD
                     KEALELALTENLQREDLNPVEVAMVYEKFLKEFDYTQEDLAKKIGIDRSSISNFIRLL
                     KLPEWIKDLMRNGKLTQGHGRVLTSLKSEREQKRFVDRVLKEGISVRELEREVKKKKA
                     QRNSSFTFVEETLRDVLKTKVNITYRKNKGKIIIEFYSKDDLERIMSIFSEKDE"
     gene            2976..3401
                     /locus_tag="PK125_10755"
     CDS             2976..3401
                     /locus_tag="PK125_10755"
                     /inference="COORDINATES: protein motif:HMM:NF012645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB38623.1"
                     /translation="MIEFNITLLIQFVNLLVLLFLLNFLLFKPVLKAINKREKTLGSL
                     SDRVEDTKQEAAKFEREYIDGTAERKKPILDGKESTLAEARQTSLSLIETARAELSQE
                     LAKVKTEIERQSKSISEALKGDVEKLSTEVAEKILKRSV"
     gene            3401..3895
                     /locus_tag="PK125_10760"
     CDS             3401..3895
                     /locus_tag="PK125_10760"
                     /inference="COORDINATES: protein motif:HMM:NF012645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit B"
                     /protein_id="HPB38624.1"
                     /translation="MSADSGSWLDWAFKIFNFAVLVAILVKFAGKPLMNFLRSRSAGV
                     QAKLDETDRLYKEAEALRNEYQARLSKLDGEIEDFKKTVQEDTDKEKKKILTEATEFA
                     NRIREQANITYQQELREMTAKIKEEIAKLTMARAEKLIIEKYNAKDHEQIVDEFIEKL
                     RSLN"
     gene            3892..4437
                     /gene="atpH"
                     /locus_tag="PK125_10765"
     CDS             3892..4437
                     /gene="atpH"
                     /locus_tag="PK125_10765"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF012439.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit delta"
                     /protein_id="HPB38625.1"
                     /translation="MIRQSIAKRYAKGLFAVGEKDGQYKGYLVELENILDLFEKEQRL
                     KKALMLPLLEVQKRKELLSDTMRISGTSLPLANMLTILLEKNRMSYLPIIKDVYSDLV
                     DDKEDRARGIVWTAFPLDPAQLKRIEEALQTRLQKKEVILSTIEDKSLIGGVKVAVKG
                     TIIDGSVKKQLEILKENILKE"
     gene            4443..5951
                     /gene="atpA"
                     /locus_tag="PK125_10770"
     CDS             4443..5951
                     /gene="atpA"
                     /locus_tag="PK125_10770"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018003165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HPB38626.1"
                     /translation="MEIKADEISRIIEQKISGFEREVNLQETGVIISIGDGIARIYGL
                     ENSMAGELLEFPQNITGMVLNLEEDNIGAVVFGEDYKIKEGDIVKRTGRIAQVPVGEA
                     IIGRVVDALGNPIDGKGPIEAKEFRNVEQTAPGVVVRQPVKEPIQTGIKAIDAMIPIG
                     RGQRELIIGDRGTGKSVIAIDTIINQKGADVFCIYVAIGQKRSSVARLVDLLTTYGAM
                     EYTTVIAATASDPAPLQYLAPFAGTSIGEYFRDTGRHALIVYDDLSKHAVAYRQLSLL
                     LRRPPAREAYPGDIFYLHSRLLERSAKWDDAHGGGSLTSLPIIETQAGDVSAYIPTNV
                     ISITDGQIYLEPELFYAGVRPAINVGLSVSRVGGNAQIKAMKQVAGRLRLELAQYREM
                     AAFAKFGSDLDRATQQLLARGSRLTELLKQGQYVPIPVEKQVVLLYAGAHGYIDNYPE
                     SALKKYEQEVLSYVENKHPDLLAEIKEKEQLDSTIEDKLTSALNNFKEQFVY"
     gene            5961..6833
                     /gene="atpG"
                     /locus_tag="PK125_10775"
     CDS             5961..6833
                     /gene="atpG"
                     /locus_tag="PK125_10775"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit gamma"
                     /protein_id="HPB38627.1"
                     /translation="MATLRDIKRKIGSINSTQTITRTMKMVSAAKLRKAQDELDKIKA
                     YALKMEELVRRVTRMLPVDSHPLLTTRDEVKKVLILSIASDRGLCGAFNVNIGLLAEN
                     FTIQNRDQYERIGVWVFGRKVKDYLARRKVDIFKEWVDIKKVDQELVEAIASELALLY
                     INGEFDKVYLSYTHFASPVKQIAVFEELLPIQTENGAETEAATFEYLYEPDRDAIVET
                     LLPKYLSTKIYYALVESQTSEHGARMSAMENATSNCGEMVRYLTLIYNKRRQENITSE
                     MMDIVGGAEALRGT"
     gene            6850..8268
                     /gene="atpD"
                     /locus_tag="PK125_10780"
     CDS             6850..8268
                     /gene="atpD"
                     /locus_tag="PK125_10780"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020045332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HPB38628.1"
                     /translation="MSEEVKGRIVQVIGTVVDIRFPSDNLPPIYGAIYVTNPTISDKP
                     DNLILEVAQHVGDSTVRTIAMETTDGLRRGQEATYKGTQISIPVGNQILGRVLNVIGN
                     PVDGGPPLSTDKMYPIHRPAPTLTMQNTKVELLETGVKVIDLLEPYSKGGKVGLFGGA
                     GVGKTVVIMEMIHNIAMHHGGISVFGGVGERTREGNDLWLEMKGSGVIDKTALIYGQM
                     TEVPGARARIGLTALTAAEYFRDEEGQDVLLFIDNIFRFTQANSEVSALLGRMPSAVG
                     YQPTLATDLGELQERITSTLKGSITSVQAIYVPADDLTDPAPATTFAHLDATTVLSRQ
                     IAELGIYPAVDPLDSTSRILDPTIVGQEHYECAREVQRILQKYKDLQDIIAILGMDEL
                     SEDDKLVVARARKIQRFLSQPFFVAEAFTGQPGRYVALKDSIRGFKEICQGLHDELPE
                     QAFYMVGTIEEAVEKAKELMGE"
     gene            8272..8682
                     /locus_tag="PK125_10785"
     CDS             8272..8682
                     /locus_tag="PK125_10785"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF009980.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HPB38629.1"
                     /translation="MAMLSLEIITPEKILVKEEVDMVEANGSLGEFGILPGHTELLTS
                     LEIGEVRYMKDGKTFTLAMSGGIAEVVNDKVTLLLDTGELAETIDVARAQIAMEEAQE
                     RLKDLTIDSAEYRKYELALLRAITRIKVASARKL"
     gene            8855..>9240
                     /locus_tag="PK125_10790"
     CDS             8855..>9240
                     /locus_tag="PK125_10790"
                     /inference="COORDINATES: protein motif:HMM:NF014594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB38630.1"
                     /translation="MIGDKTHLESLKQQYPEKFVSEKNIFKHIQAGDRIFIGTACGEP
                     QYLSKALIRYVESNPKALLDAEVLHIWSLGVAPYTDTRFKRNFRQSAFFIGEYAREAI
                     NRGEGDYSPIFLSQVPRLIRRGMIPI"
BASE COUNT         2860 a         1942 c         2100 g         2338 t
ORIGIN      
        1 caatgttacc catcctgacc atcgcttcgg taagatttaa ttatgaggca actgggcatg
       61 cgattgtttc gattagtagt aatactgtgc aaactaaggt ggcatggaaa aaactatatg
      121 ttggttgcct gtttctatat cttaaaacac cacccaaatc tatctggtcg ttctccatat
      181 tgaattccgg taatttcatc gtacaggctc tgggcagccg ggcctatttt gccgctgtta
      241 atgaccacgt taacatcgcc atggcggatt tctcccaccg gggaaatgac tgcagctgtt
      301 ccagtaccga agcactcttg cattgcccca gactttgccg tttcaatgac ctcgtcaatg
      361 gatatgcgcc gctctgacac ctttttccct ctatctttcc agagatgaat gactgtctga
      421 cgcgttatac catccagaac gcttccttca aggggaggtg taacaagctc ctcgtcgatc
      481 acaaaacaaa tattcatggt gcccacttct tctatatatt ttctttcgat accatcaagc
      541 caaagtacct gagtaaatcc cttgcgtttt gcttcttctc cggggtaaag gctagctgca
      601 tagtttgccg gtgttttagc cataccaagt cctccacgaa cggcccgaac aaattcgcct
      661 gaggtaatca atttaacagg attaatgccc tctttataat atgcaccgac aggggatgtg
      721 ataatcatga aacggtattt ttcagaaacc cttacgccaa ggaaattgtc cattgcaata
      781 atgaaagggc ggatatagag tgaatagcct ttttttctgg gtacccaacc ccggtcaaga
      841 aggagaagct cttttaagct acttatgaag gtttcaaaac ctatatcagg tatgcaaagg
      901 cgttctgatg acctcagcat ccttgcatga tatttatcag gacgaaaaac ctttatttct
      961 ccaccgtggc catagaatgc cttaaggcct tcaaaaatcg cctggccgta atggaacgta
     1021 cacagggcag ggtatatctc catgggcccg tatggaacaa tctttggact caaccacctt
     1081 ccattgtcat aatccataag caccatatgg tctgaaagaa tctcgccaaa ctgtagtctg
     1141 tcaaagtcca cactctgcaa tctgcttttg tcaatttttg tgatctcgat cttcactgat
     1201 cctcctcctt cgacggctct tgcctgaaaa atattctatc aaaccatctc tgtaatttca
     1261 attacctcgt gtatctaacc ttttgcaact catatgtttt tatggtactc ttatggacat
     1321 catggtcatc tccatttcaa accagaaagg tggcgttggc aaaacaacaa ctgcggttaa
     1381 cctgtcagca tccatagcag ttgcagagcg aagaaccttg ttggttgaca ttgatcctca
     1441 gtgtaatgcc acatcaggct ttggtatctc ctataacagg ctcgatgcaa gcctttacca
     1501 tgtccttatt ggcgaaaaaa gcattaagga tgttattcga aaaacaatga ttccttttct
     1561 tgatatcatt ccttcacatc cggaccttat aggatgcgaa attgaactcc tcgaagctga
     1621 agaaagggaa tttattctac gcaaggcgct tgatgaaata atcacggatt acgattttat
     1681 tttcattgat tgtccaccct cattaggtct tctgaccatc aatgcactcg ctgcatctga
     1741 ttatgtcatc ataccacttc aatgcgaata ttttgcctta gagggacttg ctctgcttca
     1801 acgaacaatt tccattatta agaaacgatt gaatccaaaa ctggataccc tgggaattct
     1861 tctcacaatg ttcgatagaa gaaacaacct ctctttccgt gtgtgggaag aagtcaataa
     1921 atgttttgtt gacggcgtct ttaaaacagt tattccaaga aatgtgaagc ttagcgaatc
     1981 accaagctat ggaaaaccgg tgatacttta tgatataagt tcacgagggg cagaaagcta
     2041 ccttgaactt gcctcagaaa tactggaaaa aagagggtaa atgttaaaga aggatccatt
     2101 agggagaggt ctgtcggcaa tactccagga cgtagaagaa agaggttctt caagactaat
     2161 accgatcgaa cagattattc caaatcctga gcaacccaga attgccatgc gggaagagac
     2221 tatactggag cttgcaacat cgattcagga aaagggctta ctccaaccaa tcttacttcg
     2281 cagaaaagat ggtaaatacg agataattgc tggggaacgg cgttaccgcg cttcactcaa
     2341 ggccggtctc aaagaagtac ctgccatcat taaagatgtt gacgataagg aagcactgga
     2401 actcgccctt actgagaacc ttcaaaggga agatctgaac cctgttgaag ttgctatggt
     2461 gtacgaaaaa tttcttaaag agttcgatta cacccaggag gacctggcaa aaaaaattgg
     2521 aattgatagg agctctattt ccaattttat caggcttttg aagcttcctg aatggatcaa
     2581 agatttgatg cgtaatggca agctgactca gggccatggt agggttctca catccctcaa
     2641 gagtgaaaga gagcaaaaaa ggtttgtcga tagagttctc aaagaaggta tatctgtgag
     2701 ggaactggaa agggaagtca aaaagaaaaa agcccaacgg aattcttctt ttacttttgt
     2761 ggaagagacg ctccgggacg tgctaaaaac aaaggtaaac attacgtacc ggaaaaataa
     2821 aggtaagatt attatagagt tttactcaaa agatgacctt gaaagaatca tgtcaatatt
     2881 ttctgaaaaa gatgaataaa aaggtttgca aattttttta agctgtgtta gaaaactatg
     2941 tgaaataatt cttttgacca aaaaaggagt agcgaatgat agagttcaat attacgctcc
     3001 tcattcagtt tgttaacctt ctagtattgt tgttcctcct gaattttctc ctattcaaac
     3061 ctgttctaaa ggcaattaat aagcgcgaga aaactcttgg ttcactttct gacagagtag
     3121 aggatacaaa gcaggaagcc gcaaagtttg agcgggaata tattgacgga acagcagaac
     3181 gcaaaaaacc catattagat ggaaaagaat ctacccttgc cgaggcccgg cagacatctt
     3241 tgagccttat tgaaacagcg cgggcagaac tatcacaaga acttgcaaag gtgaagaccg
     3301 agattgaaag gcaaagcaag agcatttctg aagctctgaa gggcgatgta gaaaagcttt
     3361 ccactgaagt tgcagaaaag atattgaaaa ggagtgtcta atgtccgcag attcggggtc
     3421 ttggttggat tgggcattta agatttttaa tttcgcagtc cttgtggcca tactggtaaa
     3481 atttgcaggt aaacctttga tgaatttcct tcggtcgagg agcgccgggg tacaggcaaa
     3541 actcgacgaa actgacaggc tgtataagga agctgaggct ctgagaaacg aataccaggc
     3601 acgactgtca aagcttgatg gagagattga agatttcaag aagacagtcc aagaagatac
     3661 ggataaagaa aagaagaaga tacttactga agcgactgag tttgcaaata gaatcaggga
     3721 acaggcaaat atcacctatc aacaggaact gcgcgaaatg acggccaaga ttaaagaaga
     3781 gattgcaaag cttaccatgg cccgcgccga aaagctcatt attgagaaat ataacgccaa
     3841 ggatcatgag cagatagtgg atgaattcat agaaaaacta aggagtttaa attgatacga
     3901 cagtccatcg cgaaaagata tgcgaaaggc ctattcgcag ttggagagaa agacggtcag
     3961 tataaaggtt acttagtaga attagaaaat attctggatc tttttgaaaa ggagcaacgc
     4021 ttgaaaaagg ctctcatgct tccgctcctc gaggtacaaa agagaaaaga actcctgagc
     4081 gataccatga ggatttccgg cacgtccctt cccctcgcaa atatgctaac catactcctg
     4141 gaaaaaaacc ggatgagcta tcttcctatt ataaaggatg tctatagcga tcttgtcgat
     4201 gataaagaag acagggcacg gggtattgta tggactgctt ttcccctcga cccagcccag
     4261 ctaaagagaa tcgaagaagc ccttcaaacg aggttgcaaa agaaagaagt cattctctcc
     4321 acaattgagg ataaaagcct cattggtgga gttaaagtgg cagtgaaagg aaccataatc
     4381 gatggtagtg taaaaaaaca actggaaatc ttgaaggaaa atattttgaa ggagtaacgt
     4441 atatggagat taaggcagac gagataagca ggattattga acagaagata tcgggctttg
     4501 aaagggaggt caaccttcag gaaacgggtg ttattatctc cattggtgac ggtatcgcaa
     4561 gaatttacgg ccttgaaaac tcgatggccg gggagttgct tgaattccca caaaacatta
     4621 ccggtatggt acttaacctt gaagaagaca acataggcgc tgtcgttttt ggcgaagatt
     4681 acaaaatcaa agagggtgac attgtcaaaa gaacagggag aattgcacag gttcctgttg
     4741 gtgaagccat tataggaaga gttgtcgacg cccttggcaa ccctattgat ggcaaagggc
     4801 ccattgaagc aaaagaattc aggaatgtag aacagacagc tcctggagtc gttgtccgac
     4861 agcctgtgaa agaacctata cagactggca taaaggcaat tgacgccatg attcctattg
     4921 gaaggggcca gagggaattg ataattggcg acaggggaac gggtaaatct gtcattgcca
     4981 tagatacaat catcaatcag aaaggtgcag atgttttctg catatatgtt gcaataggcc
     5041 aaaagcgctc ttcagttgca cgcctggtag acctgcttac aacatatgga gcaatggagt
     5101 acaccactgt tattgcagca actgcaagtg accctgcgcc tcttcaatat cttgctccct
     5161 ttgcaggaac atcgataggt gagtacttcc gagatactgg aagacatgca cttattgtgt
     5221 atgatgattt gtcaaaacat gccgtcgcat accgccaatt gtcgctccta ctcagacgtc
     5281 ctccagcgag ggaagcgtac cccggagata ttttctatct tcactcacga cttcttgaaa
     5341 ggtctgcaaa atgggatgat gcccatggcg gcggttcact gacatctcta cccatcattg
     5401 aaacacaggc aggagacgta tcagcatata tcccaacaaa tgttatttct attactgatg
     5461 gacagatata cctcgagccg gaattatttt acgcaggcgt aagacctgca atcaacgttg
     5521 gactctccgt atcgagggtt ggaggtaatg ctcagataaa ggcaatgaaa caggttgctg
     5581 gaagattacg gcttgaactt gctcagtacc gagaaatggc tgcctttgca aaatttggca
     5641 gcgacctcga tagagctacc cagcaactcc ttgcccgcgg gtccagattg accgagcttc
     5701 tgaaacaggg tcagtatgtg ccaattcctg tcgagaaaca ggtggtactc ctctatgcag
     5761 gagcacatgg atatattgac aattacccag aaagtgcctt gaaaaaatat gaacaggaag
     5821 tgctcagcta tgtggaaaac aagcatcctg accttctggc tgagattaag gaaaaggagc
     5881 aactggacag cacgattgag gacaaactaa ctagcgcact gaacaatttt aaagaacaat
     5941 tcgtctacta aggatacaca atggctaccc tacgggacat caaacggaag atcggtagta
     6001 tcaacagcac tcaaaccatc acaaggacaa tgaagatggt ttcagcggca aagctgagaa
     6061 aagcccagga tgagctcgac aaaataaagg cttatgcact caagatggaa gaactcgtga
     6121 gacgagttac caggatgtta ccggttgaca gccatcctct tttgacgaca cgggatgaag
     6181 taaaaaaagt gcttattctt tcaatagcct ctgacagagg tttatgtggt gcttttaatg
     6241 taaatatagg ccttctggca gagaacttta ccatacagaa cagggatcag tatgaacgaa
     6301 taggggtttg ggtttttgga agaaaagtaa aggactatct tgctcgcagg aaagtcgaca
     6361 ttttcaagga atgggtcgat ataaaaaaag ttgaccagga gcttgtggag gccatagcat
     6421 cagagcttgc gctactatac ataaatggag aattcgataa ggtctatctc agttataccc
     6481 attttgcatc acctgtgaag caaattgcag tctttgaaga gctgttgccc atacaaacgg
     6541 aaaatggagc tgaaaccgaa gcggccacct ttgagtatct ctatgagcct gatagggacg
     6601 caatcgttga aacactgctt ccaaaatatc tcagtacaaa aatttactat gctctggtag
     6661 agtcccaaac ttcggaacac ggcgccagaa tgagtgctat ggaaaatgca acgagcaact
     6721 gcggtgagat ggtaagatat ctaacactta tatataataa acgtaggcag gaaaacatca
     6781 ccagcgaaat gatggacatt gttggtggcg ccgaagcctt gagaggcaca taattaagga
     6841 ggagaataga tgagcgagga agtaaaagga aggattgtcc aggtaattgg aacggtagtt
     6901 gatattagat tcccatctga taacctccct ccaatatatg gagcaatata cgttacaaat
     6961 ccaacaatca gcgacaaacc ggacaacctc atccttgaag ttgcccagca tgttggtgac
     7021 agcacagtcc ggactatagc tatggagact actgatggtt tgcgtcgagg ccaggaagct
     7081 acctacaaag gaacacagat cagcatcccc gtgggcaatc aaattctcgg acgggtattg
     7141 aatgttatcg ggaacccagt agatggcgga cctcccctgt caacagataa aatgtatcct
     7201 attcaccggc ctgcccctac cctcaccatg cagaacacaa aggttgagct cctcgaaaca
     7261 ggcgttaaag tgattgacct cctcgaacca tattcaaaag gtgggaaagt tggacttttt
     7321 ggcggcgcag gtgtgggtaa aacagtcgtc attatggaga tgattcacaa cattgccatg
     7381 caccatggtg gtatttccgt ttttggaggc gtaggcgaaa gaacccgcga agggaatgac
     7441 ctttggcttg aaatgaaagg atcaggtgtt attgataaga ctgcactcat ttatggtcag
     7501 atgacagagg ttcccggtgc aagagcaagg ataggtctca ccgctttgac agctgccgaa
     7561 tatttcaggg atgaggaagg ccaggatgtg ctcctcttta tcgacaacat attccgtttc
     7621 actcaggcaa attcagaagt gtctgcactg ctcggcagga tgccttctgc agttggttat
     7681 cagccaaccc ttgcaacaga ccttggagag ctccaagaga gaatcacttc aacgttaaaa
     7741 ggctcaatta cttcggttca ggcaatctac gttccagcag atgacctcac agacccagcc
     7801 ccggcaacaa catttgctca tctcgacgct acaacagttc tttcacggca gattgcagaa
     7861 cttggtatct atcctgccgt tgacccactc gactcaacca gccgtatcct tgaccctaca
     7921 atcgttgggc aagaacatta tgaatgtgcc cgtgaagtac aaaggatact tcagaagtac
     7981 aaagaccttc aggacatcat tgcaatcctc ggcatggatg agctctctga agatgataag
     8041 cttgttgtgg ctcgagcccg aaaaatccaa agattccttt cacagccctt ttttgtcgca
     8101 gaagctttca ccggacaacc aggaagatat gtggccctta aggattcgat ccggggcttt
     8161 aaagaaatct gccaggggct tcatgacgaa ctacctgagc aagctttcta catggtcggt
     8221 accatcgaag aagcagttga gaaagctaaa gagcttatgg gagaatagta aatggctatg
     8281 ctcagtctgg aaatcatcac gcctgagaaa atactcgtta aagaagaagt cgatatggtt
     8341 gaagcaaatg gctctctcgg tgaattcggc attctccccg gtcatacaga gctccttacg
     8401 agtttagaaa ttggcgaggt aaggtatatg aaagacggaa aaacctttac gctggccatg
     8461 agcggaggca ttgcagaggt tgttaatgat aaggtaaccc ttcttcttga tacaggagaa
     8521 ctggctgaaa ccattgatgt ggcaagagcc cagattgcta tggaggaagc acaggaaagg
     8581 ctcaaggatc taacaataga tagtgctgaa taccgcaaat atgaactggc actgcttcgc
     8641 gctataacaa gaattaaggt cgcctcagca cggaaactgt agaacatacc tgccactacc
     8701 ttccagagtg ttatatgcat acgcatcata tactgtagga tcgtacactg taattgatat
     8761 agcagaataa tgtagcattg gatgattaca tcctcttgta cccttccatc aatgcgttgc
     8821 aaagaatcgt caattaagat aatattatgg acaaatgatt ggcgataaaa ctcatttgga
     8881 atcactcaaa caacaatatc ctgaaaaatt cgtctctgag aagaatattt ttaaacacat
     8941 ccaggctggt gacagaattt tcattggtac agcatgtggc gaaccacagt acttgtcaaa
     9001 agctctcatc cgttatgtag aatcaaatcc taaagcgctt ctcgatgctg aggtccttca
     9061 tatctggagt ctcggggtag ccccctatac agacactaga ttcaaacgaa attttcgtca
     9121 gagtgctttt ttcattggtg agtatgctcg cgaagcaatt aacagaggcg aaggtgatta
     9181 ttcgcccata tttctttctc aagtgccaag gcttatcagg aggggtatga ttcccatcga
//
LOCUS       DAOOMC010000112         9128 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdus sp. isolate AMR_MDS_2685
            NODE_8883_length_9128_cov_6.543481, whole genome shotgun sequence.
ACCESSION   DAOOMC010000112 DAOOMC010000000
VERSION     DAOOMC010000112.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864760
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdus sp. (wastewater metagenome)
  ORGANISM  Syntrophorhabdus sp.
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae; Syntrophorhabdus.
REFERENCE   1  (bases 1 to 9128)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9128)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,729
            Genes (coding)                    :: 2,708
            CDSs (with protein)               :: 2,708
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 7 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9128
                     /organism="Syntrophorhabdus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8883_length_9128_cov_6.543481"
                     /isolate="AMR_MDS_2685"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2699756"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            148..1500
                     /locus_tag="PK125_10795"
     CDS             148..1500
                     /locus_tag="PK125_10795"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF002600.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HPB38631.1"
                     /translation="MKHESGWKKKSKTEIADLFEFSEGYKKFLDEAKTEREAVRAIQD
                     ILSVSGFSQDWVADKAYSINRGKEIIVFVKGTQSLADGFNIIVAHVDAPRLDLKQNPL
                     YEDVGLGLLKTHYYGGIKKYQWVATPLALHGVLVKPDGREINLVIGENEDEPVFSIDD
                     LLPHLSRKTQDEKKLSEAIDGEKLTLLFGSIPLNGEDKDAVKKYILNILHEKYGIEEE
                     DFVSAEIEVVPAFKARDVGIDKSMIGAYGQDDRVSAYTLLKAISEVLEPKRSCLAIFV
                     DKEEIGSEGNTSMRSHFFRVFIDEMCEKLKLNCSEALIRKMLFQSRALSADVNGAMNP
                     TFQDVHEKQNACYLGRGLCITKFTGHGGKVGANDAHAEYVAEIRRIFNNKGILWQTGE
                     LGKIDEGGGGTVAKFLAEYGMDIIDCGTPILSMHSPFEISSKLDVYETYRAFKAFLRS
                     "
     gene            1521..3866
                     /gene="lon"
                     /locus_tag="PK125_10800"
     CDS             1521..3866
                     /gene="lon"
                     /locus_tag="PK125_10800"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: protein motif:HMM:TIGR00763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HPB38632.1"
                     /translation="MTELEIKATPEVLPLLPVRDMVMYPSVILPLFVGRDMSINAVEK
                     SLSKDRLILVTAQKDLTDEDPLPGRIYSVGVVSQIMRMLRLPDGRVKVLMQGLKKAKI
                     IQYVQETPTFLVRIQLIEEPIITEITLEIEALMRYVKEEMEKVVSMGRMVPPDILMVL
                     DTIDEPGKLADVAAANLGLAVDKAQEILEIVDPIERLKKLSEILGKEIELLNMQAKIL
                     SQAKEEMSKSQREYFLREQMKAIRTELGEGDERSEDVEDLRKRIKKAKMPKDIEKETK
                     KQLERLDMMHPDAVESTMLRTYIEWLIELPWNKSTKDRLDIKKAKRILDEDHYDLDKV
                     KERILEFLSVMKLKGEMKGPILCFVGPPGVGKTSLGKSIARSLGRKFLRISLGGMKDE
                     AEIRGHRRTYVGSMPGRIIQGIKTAGTNNPVFMMDEIDKIGTDFRGDPSSALLEVLDP
                     EQNNAFSDHYLNVPFDLSKVMFITTANRVDTIPSALKDRMETIYISGYTDNEKLAIAK
                     KYLVPKQLAENGLTPAMIEFADKALTKIIQDFTQEAGLRNLEREIAAISRKVAKKIAE
                     GQKEKTVVTLKNLHSFLGPSKYLPETGIEVDSVGIASGLAWTEFGGDVLYIEASCRKG
                     KKELMLTGSMGDVMKESAQAALTYIKSKAAALGIEQDIFDTLEMHVHIPQGAIPKDGP
                     SAGITMAVAMISAITKHPLSRKIAMTGEITLRGRVLPVGGLKEKTLAALRARIKKVIL
                     PEENMRELVEIPQYVKKKINFLPVKTMDDVIKIMFVNKTVG"
     gene            3894..4907
                     /gene="amrS"
                     /locus_tag="PK125_10805"
     CDS             3894..4907
                     /gene="amrS"
                     /locus_tag="PK125_10805"
                     /inference="COORDINATES: protein motif:HMM:TIGR04337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system radical SAM enzyme"
                     /protein_id="HPB38633.1"
                     /translation="MKEALFYENLENQKVHCFLCRHHCEINDGKRGICGVRENRGGTL
                     YTLVYGFPCSHHVDPIEKKPLFHFYPGSKAFSIATVGCNFRCRHCQNHEISQIRKDDG
                     HIYGQRMTPSEVVEGALKSGCKSISYTYTEPTIFFEYALDCAKLSKEKGICNNFITNG
                     YIEQEPLKTIRPYLDGANIDLKSFNHDFYKKVCGAELDNVLASIRTFRSLGIWIELTT
                     LVIPGYNDSEDEFRAITRFIKNDLGTETPWHVSAFYPTYKLTDVSRTPAKTLQRAREI
                     GFEEGLRYVYTGNIPGEDGENTYCWSCKKLVVGRYGYTISEYNIKDGACLFCGAIIDG
                     VGM"
     gene            5194..7638
                     /locus_tag="PK125_10810"
     CDS             5194..7638
                     /locus_tag="PK125_10810"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF001950.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-translocating pyrophosphatase"
                     /protein_id="HPB38634.1"
                     /translation="MSVVKRFHPVSVLSFFSMMLLLQVSGAFAGEADIILPDLSTVKF
                     SIFGGLNGLSIMYFGILVCFIGLIFSITQASQTKNLPCHKAMLEVSEIIWQTCKSYLA
                     QQGKFLIILWVLIAICMVYYFMGLSHAPFGNMVIILLCSILGILGSYGVAWFGMRINT
                     WANSRTAFSSLRGKPVDVVNIPLTSGMSVGLLLISVELFFMICILVFLPKDLVGPSFI
                     GFAIGESLGASALRIAGGVFTKIADIGSDLMKIVFHLPEDDPKNPGVIADCTGDNAGD
                     SVGPTADGFETYGVTGVALVAFLALALATTPLMSAILIIWIFSMRILMVLTALVSYFV
                     NKGITNSLYGSKTKFDFEQPLTNLVWLTSIISIAVTFWASYMLLGEQNAQYPGLWWAL
                     ATIISCGTIAGALIPEFTKIFTSTKSRHVQEVVSAARHGGASLDILSGFVAGNFSAFW
                     EGLVILFLMFIAYMVSQHASILAIMPPQFTFAAPVFAFGLVAFGFLGMGPITIAVDSY
                     GPVADNAQSVYELSRIESVPNVKEEIKKGFGFEPNFDVAKDYLEEADGAGNTFKATAK
                     PVLIGTAVVGATTMVFGIIILLENMYGNVIAHLSLVNPKIILGLLMGGAVIYWFTGAA
                     TQAVTTGAYRAVVFIKKNINLDKAEASIEDSKEVVKICTQYAQKGMINIFIVIFFLAL
                     ALSFFNPYFFIGYLIAIAFFGLFQAIFLANAGGAWDNAKKIVEVDLKEKGTSLHEAAV
                     VGDTVGDPFKDTSSVSMNPVIKFTTLFGLLATEIAVTMTSQGLKTGLGIVFLIISLIF
                     VYRSFYGMRVGEEKLE"
     gene            complement(7834..>9128)
                     /locus_tag="PK125_10815"
     CDS             complement(7834..>9128)
                     /locus_tag="PK125_10815"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024732.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HPB38635.1"
                     /translation="VPSDVAERIGKYVASIVEDGDTIQVGYGSTPNAIIAALSNKHNL
                     GVHTELFSHGIADLMKQGVVNNTKKRIDHSKTVAAFCMGNKETYEFIHDNPAVEFRTI
                     DYTNNPAVIAALGNITAINSALEIDLTGQATAESIGKTFYSGIGGHTDFMRGAVLTPN
                     GKSVLVLQSTAQDGTVSRIVPYLREGAGVTLTRGDVHYVVTEFGIAYLHGKNIRERAM
                     ALIAIANPKFQPWLIEEAKKQALIYKDQAFICGKGGEYPEELELYKTTRSGLKILLRP
                     VRISDEPLLKNFYYSLSESTVYKRFMSPRKDMPHEKLQQFFVVIDYTKEMVMLAILKQ
                     KDQEVVAGVGQYRGHEESHSAEVAVMVKDEYQNQGIGTELLEHLTIIARKQGLLGFSA
                     EVLKENRIMMHLFEKMGFVIKKEVLPDAYTLRLGFREN"
BASE COUNT         2530 a         1981 c         2129 g         2488 t
ORIGIN      
        1 ccttaacaat gttctggccc tcattgcccg tcctgctatg tggagtcttt tgcgtgcgtc
       61 cggtttggtt aaacgaattc ttggctgatg tcggctggat tgacttccgg ggttgatttc
      121 tgttaatcta aggcacaaaa attgcctatg aaacatgaat ctggctggaa gaaaaaatct
      181 aaaacagaaa tagccgacct attcgaattc tcagagggat acaaaaagtt tcttgatgaa
      241 gcaaaaactg agagggaggc tgtgcgcgct atccaagaca tcctttccgt ttctggcttc
      301 tcccaggact gggtggcaga caaggcgtac agcatcaaca ggggcaagga aatcattgta
      361 tttgtaaagg gaacacaaag ccttgccgat ggtttcaaca ttattgtagc ccatgtagat
      421 gcaccaaggc ttgaccttaa acagaatccc ctatacgagg atgtcggttt agggcttctt
      481 aaaacacatt attatggagg tatcaagaaa tatcaatggg ttgccactcc tcttgccctc
      541 catggtgttc tcgtgaaacc agatggtaga gagattaacc ttgtaatcgg agaaaatgaa
      601 gatgaaccgg ttttttctat tgatgatctt ctcccccatc tgtccagaaa gacacaggat
      661 gagaagaagc tatctgaagc tatcgacggt gaaaagctga cccttctttt cggaagcatt
      721 cctttaaacg gggaggacaa agatgctgtg aaaaaataca tcctcaatat tttgcacgaa
      781 aaatacggaa ttgaggaaga agatttcgtc agcgcggaga tagaggttgt accggccttc
      841 aaagcgagag atgtgggtat tgataagagt atgataggtg cctacggtca ggatgaccgg
      901 gtaagtgcgt atacccttct caaggctatt tctgaggtgt tagaaccgaa aagatcttgt
      961 ctggcaattt ttgtggataa ggaagaaatc ggatcagagg gcaacacaag tatgagatca
     1021 cacttttttc gcgtctttat tgatgagatg tgtgaaaaat taaaactgaa ctgcagcgag
     1081 gcccttattc gaaaaatgct ctttcagtca agagctcttt ctgctgatgt caatggggct
     1141 atgaacccca ccttccagga tgttcacgag aaacagaatg catgctatct cggtcgagga
     1201 ctctgtatca caaagtttac tggtcatggc ggtaaggttg gagcgaacga tgcccatgca
     1261 gagtatgttg cagagataag aaggattttt aacaataagg gtattctctg gcagacagga
     1321 gaacttggta aaattgacga aggaggaggt ggaacagtgg caaaattcct tgccgaatat
     1381 ggcatggaca ttatcgactg tggtacgccc atcctttcaa tgcactctcc ttttgagata
     1441 tcgagcaaac ttgatgtcta cgagacatac agggccttta aggcctttct cagatcgtaa
     1501 taaacggagg cttaacctaa atgaccgaac ttgaaataaa agcgacgcca gaggttcttc
     1561 ccctgttgcc ggtaagggac atggtgatgt atccttcagt aatccttccc ctctttgtgg
     1621 gaagagacat gtcgatcaat gctgttgaga aatcattgtc gaaagaccgg ttgattcttg
     1681 tgacggctca aaaagatctc accgatgagg atccccttcc tggcaggatt tattctgtcg
     1741 gtgttgtatc gcagattatg aggatgttgc gccttcccga tggtagggta aaggttttga
     1801 tgcagggact gaaaaaggca aaaatcatac aatatgttca ggaaacgcct acattcctcg
     1861 ttcgcattca gctaattgag gagcccatta taacggaaat aaccttagaa atagaagctc
     1921 tcatgcggta cgtgaaagag gagatggaaa aggttgtttc tatgggcagg atggttccac
     1981 cggacattct gatggtcctt gataccattg atgaaccggg aaagcttgct gatgtggcgg
     2041 ctgcgaacct cggcctagca gttgacaaag ctcaggaaat cctggaaata gttgatccta
     2101 ttgaaaggct gaaaaaactc tctgagatcc tcggaaagga gatagaactc ctcaatatgc
     2161 aggcaaaaat tctttcccag gccaaggagg aaatgtccaa gagccagcgg gaatactttc
     2221 tgcgagagca gatgaaggcg atacgcactg aacttgggga aggtgatgaa cgctcagagg
     2281 atgtggagga cctcagaaag cggatcaaga aagcaaagat gccgaaggat attgaaaaag
     2341 agaccaagaa acagcttgag cgactcgata tgatgcaccc tgatgctgtt gaatcgacca
     2401 tgctcaggac ttacattgag tggcttattg agcttccatg gaacaaatct acaaaagata
     2461 ggctggatat caagaaagca aagaggatcc tagacgagga ccactacgat ctcgacaaag
     2521 tgaaggaacg tattcttgag ttcttaagcg ttatgaagtt aaagggggag atgaagggcc
     2581 ccattctctg tttcgtgggt cctcccggtg tgggaaaaac ctctcttggc aaatctattg
     2641 cacggtcctt gggaagaaaa tttctgagaa tctcattggg tggaatgaaa gatgaggcag
     2701 agataagagg ccatagaagg acttatgtgg gttccatgcc agggaggatt attcagggca
     2761 ttaagaccgc cgggactaac aatcctgttt ttatgatgga tgagattgac aaaattggca
     2821 cagattttcg gggtgaccca tcaagcgccc tccttgaggt tttagatccg gagcagaaca
     2881 atgccttcag cgaccattat cttaatgtac cctttgatct ttccaaggtt atgttcatta
     2941 caaccgcaaa tcgtgtagat accattccat cagccctgaa agacaggatg gaaaccattt
     3001 atatatcagg ttatacagac aacgaaaaac ttgctattgc aaagaaatac cttgttccga
     3061 aacaattggc agagaatggc ctcactcctg ctatgatcga atttgcagat aaagcactca
     3121 caaaaatcat acaagatttc acgcaggaag ctgggctaag aaaccttgag agggaaattg
     3181 cggccattag cagaaaggtg gcaaagaaga tagcggaggg tcaaaaagaa aagacggtcg
     3241 taacacttaa aaatcttcat tcttttctgg ggccgtcaaa gtaccttcct gaaacaggga
     3301 tagaagtaga ttctgtagga atagcaagcg gtctggcctg gaccgaattt ggcggagatg
     3361 tcctgtatat agaggcatcg tgcaggaaag gaaaaaaaga gcttatgctc actggcagca
     3421 tgggggacgt aatgaaagag tcggcccagg ctgcccttac ctatattaag tcgaaggcag
     3481 ccgctctcgg catcgaacag gatatcttcg ataccctcga gatgcacgta catatccccc
     3541 agggcgcgat ccccaaggat ggtccatcag ctggtatcac tatggcagtg gccatgatca
     3601 gtgccattac gaaacatcca ctaagcagga aaatagccat gaccggagaa atcaccctga
     3661 ggggcagggt tctccccgtc ggaggattga aagagaagac ccttgcggcg ctacgggctc
     3721 ggattaaaaa agttatactc cctgaggaaa acatgaggga attggtcgaa ataccgcagt
     3781 atgttaagaa gaaaataaat ttccttcccg tgaagaccat ggatgatgtt atcaagatca
     3841 tgtttgtaaa taagactgtg ggatgattgt gtcaggtttg ttggtgagat gacatgaaag
     3901 aagccttgtt ttatgaaaac ctggagaatc aaaaagtcca ttgcttcctc tgccggcacc
     3961 actgcgaaat caatgatggg aaaaggggaa tttgtggagt tcgggagaac agaggcggaa
     4021 cattatacac ccttgtttat ggatttccct gttcgcacca tgttgaccct attgaaaaaa
     4081 aaccgctctt tcatttttac ccaggttcca aagcattctc gatagcgaca gtgggttgta
     4141 attttcggtg ccgccactgc cagaatcatg aaatctctca gatacgaaaa gatgatggcc
     4201 acatctatgg tcagagaatg acgccatcgg aagttgtgga aggagcccta aaatcaggtt
     4261 gtaagagtat ttcctacacc tacactgaac cgacaatttt tttcgaatat gctctcgact
     4321 gcgccaaact gtcaaaggaa aagggcatct gcaacaactt tatcaccaac ggctatattg
     4381 agcaagaacc attaaaaact atcaggccat accttgacgg ggcaaacatc gatctaaaga
     4441 gttttaacca tgatttctac aaaaaggtct gtggggcaga gctcgataac gttcttgctt
     4501 ccataaggac attcagatcc cttggtatat ggatagagct gaccaccctc gtgattcccg
     4561 gatataatga tagcgaggat gaatttagag ccattacccg gtttattaag aacgatctcg
     4621 gtactgaaac tccctggcat gtcagtgcct tctatccgac ttacaagctg accgatgtat
     4681 cgagaacgcc tgcaaagaca ctccagaggg cgcgcgagat cggattcgag gaggggctca
     4741 ggtatgtgta cacaggaaac attccaggag aagatggcga gaatacctac tgctggagct
     4801 gtaagaagct cgttgtcggc cgctatggct atacaatttc cgaatacaat atcaaagatg
     4861 gagcctgcct tttctgtggg gcaatcatcg atggtgttgg aatgtaaaat attcaaaaat
     4921 ggccgattgt gattcagcgt cgggatagca gattatttta aattctaaaa gattctcaga
     4981 cacggtgaag atttcttacc cagcacacaa ggcctctcaa aacagagcaa taattgcaaa
     5041 atttaattgt gaattttttc ataagttaat tgacattgca gattaaaaag ttttaaaata
     5101 aaataatctt tcaataagca catgaaaatt aggtaacaaa cttgggttac agggtatttt
     5161 aagtatatta aaatttttgt aaggaggttg gagatgagtg ttgtaaaacg gtttcacccg
     5221 gtgtcagtgc tatccttttt cagcatgatg cttctcctgc aagtatctgg tgcttttgca
     5281 ggcgaggcgg acatcatact accggatctt tccacagtga aattttcgat ttttggtggt
     5341 ttgaacggtt tgtccatcat gtatttcggt attcttgttt gtttcatcgg ccttatcttc
     5401 tcgataactc aggcatctca gacaaaaaat cttccgtgcc acaaagccat gcttgaagta
     5461 tcagaaatca tctggcaaac atgcaaatcc tatctagccc agcagggtaa attcctgatc
     5521 atcctctggg tacttatcgc gatctgtatg gtttattatt tcatgggatt gagccatgca
     5581 ccattcggca atatggtcat tatcctttta tgttctattc ttggtatcct tggttcttat
     5641 ggtgtagcat ggtttggcat gcgcatcaac acatgggcga actccaggac cgctttctcg
     5701 tcccttcgag ggaagcccgt tgatgttgtc aacatccccc ttacctccgg aatgagcgtg
     5761 ggactcctcc ttatcagcgt tgagcttttc ttcatgatct gtatccttgt gttccttcca
     5821 aaagatctag tcggtccaag ttttataggt tttgctatcg gtgagtccct cggtgcctct
     5881 gcactcagaa tcgccggtgg tgtcttcacg aagattgccg atatcggctc tgaccttatg
     5941 aagatcgttt tccacctgcc tgaagatgac cccaagaacc ccggtgtcat tgccgactgt
     6001 accggtgaca acgcaggaga cagcgttggt cctacagcag atggattcga aacatacggt
     6061 gtaaccggtg tagccttagt tgcctttctc gcccttgcat tggcaacgac tccccttatg
     6121 tcagcaatcc tgatcatctg gatattctca atgcgtatcc tcatggtcct cactgccctc
     6181 gtttcatact tcgttaacaa aggtatcacc aattccttgt atggctcaaa aacaaaattt
     6241 gatttcgaac agcccctcac caacctcgtt tggcttactt cgataatctc aatcgctgtc
     6301 accttctggg caagctacat gctccttggc gagcaaaacg cccagtatcc tggcttatgg
     6361 tgggcacttg ccacaatcat ttcgtgtggc acaattgccg gtgctttgat tccggaattc
     6421 acaaagatct ttaccagtac caagtccagg cacgtccagg aagtagtcag tgctgccagg
     6481 catggaggtg catccttaga tattctgtca ggttttgtgg caggcaactt ctccgcattc
     6541 tgggaaggac ttgtgattct ttttcttatg tttatcgctt atatggtgtc tcagcacgcc
     6601 tcaattctcg ctattatgcc tcctcaattt acctttgcag cgcccgtttt cgcgttcggc
     6661 cttgtagcct tcggttttct cggcatgggt cccatcacaa tcgctgttga ctcatatggc
     6721 ccagttgctg acaacgccca gtcagtatat gagctttcca ggatagagag cgtacctaac
     6781 gttaaagaag agataaagaa ggggtttggt ttcgaaccca attttgatgt tgctaaggac
     6841 tacctcgaag aagcagatgg ggcaggcaac acgtttaagg caacagcaaa gcctgtgctg
     6901 atcggtacag cagtcgttgg tgctaccacc atggtctttg gcatcatcat actccttgaa
     6961 aacatgtacg gaaatgttat tgcacactta agtcttgtca atcccaaaat aattcttggg
     7021 ctccttatgg gtggggcagt aatctactgg tttacaggcg ccgcaacaca ggcggttacg
     7081 accggtgcgt accgtgcagt cgtttttatt aaaaagaata tcaacctaga caaagcggag
     7141 gcatctatag aggacagtaa agaagttgtt aagatttgca cacagtacgc gcagaaaggg
     7201 atgatcaaca tcttcatcgt catttttttc ctcgctctgg ccctctcttt cttcaacccg
     7261 tatttcttta tcgggtacct cattgcgatt gcctttttcg gtcttttcca ggccatcttt
     7321 cttgccaacg ccggtggtgc ctgggacaac gctaagaaga ttgttgaggt tgaccttaaa
     7381 gaaaagggca cgtcccttca tgaagctgca gtcgtcggcg acacggttgg agaccccttc
     7441 aaggatacat cttcagtttc aatgaaccca gttattaagt ttacgaccct tttcgggctg
     7501 ctcgcgacag agattgcagt gaccatgact tcccagggcc ttaaaacagg tctgggtata
     7561 gtgtttctca ttatatcact gatctttgta taccgatcct tctatggtat gagagtaggt
     7621 gaagaaaagc ttgagtaata aaacattctg acaaatagaa aaaaggcgct tcttttgaag
     7681 cgcctttttt ctattaaaag agggatgagt aataactgat ccgcggatcc cgataaaact
     7741 accggggatt atggtactca acatccagta agaacgagta caaccaagaa cagcataata
     7801 acaactttca ccattggagc cctcccacaa aaattaattt tctcgaaagc cgagtcttaa
     7861 agtgtatgcg tcaggcaaaa cctctttttt tatgacaaaa cccatttttt caaaaaggtg
     7921 catcataatc ctgttctctt tgagtacctc agcggaaaaa ccaagcaagc cctgttttcg
     7981 tgcgataatg gttaggtgtt caagaagttc tgtgccgata ccctggttct gatattcatc
     8041 cttgaccata acagctacct ctgcagaatg gctctcctcg tgccccctgt actgaccaac
     8101 gcctgcgact acttcctgat ccttctgttt caaaatagca agcatgacca tttcttttgt
     8161 atagtcaata acaacaaaga actgctgcag tttctcgtga ggcatatcct ttcgcgggga
     8221 catgaatctc ttgtataccg ttgattcgga caaggagtag tagaaattct ttaaaagagg
     8281 ttcgtcactt atccgtactg gtcgaagaag aatcttcaaa cctgaccgtg tggttttgta
     8341 gagctcgagc tcttcagggt attcaccacc ctttccgcaa ataaatgcct ggtctttata
     8401 aatcaaagcc tgtttttttg cttcttctat gagccatggc tgaaatttgg gattggcaat
     8461 tgcaatgagg gccatagctc gctctctaat atttttaccg tggagataag cgataccaaa
     8521 ctcggttaca acatagtgta cgtctccccg tgtaagagtt acgcctgctc cttctctcaa
     8581 ataaggcaca atcctcgata ctgtgccatc ttgtgcggtt gattgcagaa caaggacgct
     8641 cttgccgttg ggcgtgagta ctgccccgcg cataaaatct gtgtgcccgc caatgccact
     8701 ataaaaagtt ttaccgattg actctgccgt tgcttgtccg gtgaggtcta tctcaagggc
     8761 gctgttgatt gctgtaatat ttccaagtgc tgcgattaca gccgggttat ttgtgtagtc
     8821 tatagttctg aattcgacag caggattatc atgaataaat tcatatgtct ctttattgcc
     8881 catgcagaag gctgcaaccg tcttactgtg gtcaatacgt ttcttggtat tgttcactac
     8941 cccttgtttc ataagatctg cgattccatg ggaaaacaat tcagtatgca cgcccagatt
     9001 atgtttgttg gaaagcgctg ctattattgc gtttggtgta gacccataac ccacctgtat
     9061 tgtatcgcca tcttcaacga tactggcaac gtattttccg atccgttcag ccacatcact
     9121 gggtactg
//
LOCUS       DAOOMC010000113         9090 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdus sp. isolate AMR_MDS_2685
            NODE_8928_length_9090_cov_6.623907, whole genome shotgun sequence.
ACCESSION   DAOOMC010000113 DAOOMC010000000
VERSION     DAOOMC010000113.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864760
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdus sp. (wastewater metagenome)
  ORGANISM  Syntrophorhabdus sp.
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae; Syntrophorhabdus.
REFERENCE   1  (bases 1 to 9090)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9090)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,729
            Genes (coding)                    :: 2,708
            CDSs (with protein)               :: 2,708
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 7 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9090
                     /organism="Syntrophorhabdus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8928_length_9090_cov_6.623907"
                     /isolate="AMR_MDS_2685"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2699756"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1445)
                     /locus_tag="PK125_10820"
     CDS             complement(<1..1445)
                     /locus_tag="PK125_10820"
                     /inference="COORDINATES: protein motif:HMM:NF012686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HPB38636.1"
                     /translation="MIWIVLILGVILVLISAYGYKLSSKTAADYLLAGRAIGTFVMFF
                     YVYFAISSAWTFYGFPGTIYNTGPGFMLFFSFISFAFLYVLIGPKLWAGGKKYGYISP
                     VQYLGERYQSNALRLIVSGLLVLFLFPYLGLQAVGIGAGMKAAVGLPFWFGALYMTVI
                     MLLICLIGGTRSVAWLNVLLGIIFTATFWGFLVWIMFATDNSSLTAMAEKVKVLKPGL
                     LGVPGPVNAWTTQSLMGLALAGLTVFAWPHIVIGTMQAKSLVVVRKTAIAMLLFSIFF
                     CNIAAIWGYLVAPILIPGLKGKAADAVVQMAITQYLPVWASGATLIAVLAAAISTVAT
                     QLMITGLLVSRDIVFAVSKPSDKALILWSRYAMIGASVVSFALAMARPAELGLLLSNI
                     ASPGFAMWIPALLGGVLWKRGTKEGAITGLLVGLALLIGGSYFYKPLLLGFHPIFIPL
                     LVNTVLYIGVSLVTSPVSKEVQHKFFDELED"
     gene            complement(1778..3352)
                     /locus_tag="PK125_10825"
     CDS             complement(1778..3352)
                     /locus_tag="PK125_10825"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HPB38637.1"
                     /translation="MGQQKQFSTIRTLLDRNAMLYPDKIAMKEIDGTRAYTYKELKDR
                     TNRMGNALYDLGIKKGERVAILSQNSFEYIESSLSVPNAGLIYVVCNFRLAPPEIASV
                     LADAEPSILFVQDQFVDIAQNLKSFLPSVKHFVYFGDPAKKPDGWLDYEGLIRNASPS
                     DQTSDIFEDDIAMLMYTSGTTGLPKGVMQTHGNFYHAGRVCSRNNNLSMDDHVFVVCP
                     MYHITAHYTFFGSFYVSAPAYIFPRWDVDLFLSTTEKEKLTAGMFATPMVMMILDSPN
                     LKKYNVSSWRNLWFAGAGIIPSVYKQFIDTFGNILGEHHGTTESTGVTTNLSPRDIQD
                     AFDRGDFNILESCGKASYDMEILVVDENNTPVPSGGIGEMIIRGPGISLGYWRKEEAT
                     KKAFQGEWFHTEDVCSIDEWGYIRVIDRLKDMIITGGENVYPAEVEKVLHQHPYVKES
                     CVIGTPHPVWGEAVTAVIVLRNGEAVTPSDFINYCKGKIAGYKVPKIIHFIPEMPRNA
                     AGKIMKRELREQFSKN"
     gene            3698..5788
                     /locus_tag="PK125_10830"
     CDS             3698..5788
                     /locus_tag="PK125_10830"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HPB38638.1"
                     /translation="MNEKKGYELYRDRANKEHEARMFWQPRVNGWLRKRLPGASGIKS
                     KSLIRNFRNAPIFPEQTPAERAHRESENKFRDLAEKSLVGIYLIQDDVFKYVNPKLAE
                     IFGYSIDNLTGKKRKTPKDLVLPEDWPLVQDNIRKRISGETESIHFEFRGITRKKEII
                     YVEVYGSRTVYQERPALIGTLLDITGRKKAHEKLQQTEKKYRSIFENAVEGICQTTPE
                     GKPISANPAFAKMLGYDSPDEFLSSVADVRNQIYVHPEIRTNFMESIERDGLVRGFEC
                     ELYRKDGSQILVSISARDVRDENNRILYYEGTVEDITEKKKVERELEMLHQLNTSIIE
                     HAPVAIFTIDMQGKFMSVNPALATLSGLGREAEEKLLGFNWLENQFTIRCGLAEYIRK
                     GLQGEPFQLWDFAFMTYRGDRNIFIDFKGVPLKGKDGEIEGLLCIIEETTERVKTRAK
                     LMQEVKMATIGRLSAGIAHELNNPLGTLVAYAERASNYLESIDNIFLSQYEFEKLKGY
                     LKIIDEEAFRCKRVVSDILSIPKNEGQEITRVDIERLLDTILERLSAENPGLSITRET
                     MALLPVIPGDTNILRQIFVNLINNALDALEGRKDAQLCIRTALRNNMVVVEVEDNGIG
                     IPDTIIEKIFEPFFTTKESKKGIGLGLSLCYDFVKGMGGTIEVHSGPASGTNFVVSLP
                     IDQDRKKREEGNHD"
     gene            5781..7133
                     /locus_tag="PK125_10835"
     CDS             5781..7133
                     /locus_tag="PK125_10835"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HPB38639.1"
                     /translation="MINVLIVDDEIRLAEAFREQLTEEGMYVTVAHRAKEALSAMKRE
                     NFDVAVLDIKLPDMDGVELLLKLKQLEPTTEVIMLTGYASVATAIRSMKLGAYDYLTK
                     PCKMTELSNVITKAHEKKALKVKNIILEEQLHRIGGSNDFVGNSKEMEKVRGQISMVA
                     GSDIPVLILGETGTGKELAARAIHDLSPRAINPFVVVNSSILQEAMLESELFGYRKGA
                     FTGAETDKMGLIEIADRGTFFIDEVGDMGMSIQAKLLRVLETGIFRKLGDTKEIKVDV
                     RFIFATNKDLNVAAEEKQFRRDLFFRISTFVINLPPLRGKREDIPLLVRYFLEKCSRG
                     GKKKHLSREAMELLMAYDWPGNVRELSNVVERAAIVSNDREEILAQDLSEGMLNPVSQ
                     VKESKDSQIKGRILTLREMEAEYIRNVLQSVGGNKSRAARLLGISRKNLYDKINVSHS
                     "
     gene            7242..7544
                     /locus_tag="PK125_10840"
     CDS             7242..7544
                     /locus_tag="PK125_10840"
                     /inference="COORDINATES: protein motif:HMM:NF024176.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB38640.1"
                     /translation="MVFGKKKHITMFDEVLESSWKLLHEGVQSFRSPFHHAALTTIDG
                     DKPRAQLFILREFSEKNRIFICHCDIRGCKVLQIRDNRNVSRLFYDSEKWLQVRLS"
     gene            7690..8136
                     /locus_tag="PK125_10845"
     CDS             7690..8136
                     /locus_tag="PK125_10845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB38641.1"
                     /translation="MKMINKHYWVVGQVTRAEVMLVGNGGGHILGMFESRGLAHIGNE
                     VVVVTSWVQGDLLNGNGPIRGYTRYMYEDGSTIISKTEYTCKSNPESKAHLYENGYGE
                     FLSGSGRFYGIKGSSSWKGRQVTPISDETKGDWIVEGDMVYTLPPE"
     gene            complement(8170..8832)
                     /locus_tag="PK125_10850"
     CDS             complement(8170..8832)
                     /locus_tag="PK125_10850"
                     /inference="COORDINATES: protein motif:HMM:NF017009.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB38642.1"
                     /translation="MQAKPAYALEMKVGQLLVKRGFLDEGQLEEALTVQRKLKEYKPL
                     GEICKELGFISGRVLRDFLSRYQKQIFLGELINKMGIISDEQLDEALQQQKKSGEKLG
                     QILIKNGMITSAVLIDSLCVQLGIEKMHPRKDHVDRNLLDEANHAYFRKKRVIPLQLD
                     KTKRVLTVVMEDPTDNEAIGDLQKMFNASVEPFIGPPGVTEFLLNEIFDVWYVSHSHR
                     RA"
BASE COUNT         2708 a         1899 c         2143 g         2340 t
ORIGIN      
        1 taatcctcta attcatcaaa aaacttgtgt tgcacctctt ttgacacagg ggatgtaacc
       61 aaactaacac caatatacag aacggtattt actaaaaggg gtatgaagat tggatgaaaa
      121 cctaaaagca atggtttata gaaataggat ccacctataa gcaaggccag acccacaaga
      181 agcccggtga ttgccccttc ctttgtgccc cgtttccaca agacgccacc taaaagtgca
      241 ggaatccaca tggcaaatcc tggtgaagca atgttgctca gcaatagccc taactctgca
      301 ggtcttgcca tcgccagggc aaagctgact accgatgcgc caatcatggc ataccttgac
      361 caaagtataa gcgctttgtc gcttggtttt gacacggcaa aaacaatatc cctcgacacg
      421 agaagcccgg taatcatcaa ctgtgttgca actgtggata tagcggctgc gaggacagcg
      481 ataagagtgg caccgctcgc ccatacaggc agatattggg tgatagccat ttgtaccact
      541 gcatctgcag ccttcccttt gaggccggga atcagtatgg gcgctaccag atatccccag
      601 atggctgcaa tattgcagaa gaatatggag aacagaagca ttgcaatggc tgtcttgcgg
      661 acaacaacaa gactctttgc ctgcatagtg cctatgacga tatgaggcca ggcgaacaca
      721 gtcaggccag caagggcaag ccccatgagg ctctgggtag tccaggcatt cacaggtcca
      781 ggtacaccga gcagaccggg tttcagcacc tttacctttt cagccatggc tgtaaggctt
      841 gagttgtccg tagcaaacat aatccacacc agaaaacccc agaaagttgc ggtaaaaata
      901 atgccgagga ggacgttcaa ccaagcaacg cttcgcgttc cgcctatgag gcagatgaga
      961 agcatgatga cagtcatata cagagcgcca aaccagaagg ggagacccac tgctgctttc
     1021 attcctgcgc cgatgccaac tgcctgaagt ccgaggtacg gaaagaggaa taatacgagc
     1081 agcccgctga caataagccg cagggcattg gattgataac gttcgccgag atactgaaca
     1141 ggtgatatat atccgtactt ttttccacca gcccacagtt ttggaccaat caaaacgtaa
     1201 aggaaagcaa acgaaataaa agagaagaaa agcatgaatc caggcccggt attataaatt
     1261 gtcccgggaa atccataaaa ggtccaggca cttgatatgg caaagtagac ataaaaaaac
     1321 atgacaaacg ttccgattgc ccttccggca aggagataat cggctgcagt cttgcttgac
     1381 agtttatacc cgtaagcact gataagcacc aggattacac ctaagataag aactatccag
     1441 atcataatat cctcctccgt tgcattcttt tttagaaaag cctttttgtt cccattatgc
     1501 tcaccggtat ggccagtacc ttgatgtgta aatccagaat acaacaagcc ataaagttgt
     1561 ctgggcagta taaaagaata caacgaccgg catccaatca aagaggagat aggtggtatt
     1621 ttgcagcacg aatggaagaa tgaaaatgta ccagatagag cttaaaccta gaatccacaa
     1681 gaagattttc cctggtctgt gacctgttat ttctctcata acaatacccc cttagcgtgt
     1741 aatacgggcc aggaattatc ctggcctaaa aataagctta attcttcgaa aactgttccc
     1801 gtagttccct tttcataatc tttcccgcag cattcctggg catttcagga ataaagtgga
     1861 taattttagg taccttgtat ccggcaattt tccctttaca gtaattgata aagtccgatg
     1921 gtgttaccgc ctccccattc ctgagaacaa ttaccgcagt cacggcctct ccccaaacag
     1981 ggtggggcgt gccaatgacg cacgattcct taacataggg atgctgatga agaacctttt
     2041 caacctcagc agggtataca ttctcgccac cggtaatgat catatcttta agcctgtcta
     2101 ttaccctgat atatccccat tcatcaatgg agcagacatc ctcggtgtga aaccactccc
     2161 cctggaatgc ctttttggtt gcttcttcct tcctccagta acccagagat atgccaggcc
     2221 cccgtattat catttctcct ataccgcctg aaggaacagg agtgttattt tcatccacaa
     2281 caagtatctc catgtcgtaa cttgcctttc cgcatgattc aagtatattg aaatcgcccc
     2341 tgtcaaaagc atcctggata tcccgcggag aaaggttggt ggtaacgcct gttgattcag
     2401 tggtaccatg gtgttccccg aggatgttac cgaaagtatc aatgaactgt ttgtaaacag
     2461 atgggattat ccctgctccg gcaaaccaga ggtttctcca gcttgaaaca ttgtattttt
     2521 tcaggttcgg ggaatcgaga atcatcatca ccattggagt agcgaacata ccagctgtaa
     2581 gtttctcttt ctcagtggtt gaaaggaaaa ggtcaacatc ccaccgggga aagatatagg
     2641 caggcgccga tacatagaag ctaccgaaga aggtataatg ggcagtgata tggtacatgg
     2701 ggcagaccac aaacacgtgg tcatccattg aaagattgtt gttccgggag cagacgcgac
     2761 cggcatggta gaaatttccg tgggtctgca ttactccctt gggaagcccg gtggtcccgc
     2821 ttgtatacat gagcattgca atgtcatcct caaagatatc tgatgtctgg tcagaaggtg
     2881 aggcatttct tatcagccct tcatagtcaa gccagccatc aggttttttt gcaggatcgc
     2941 cgaagtatac aaagtgtttg acagatggaa gaaagctttt taggttttga gcaatgtcaa
     3001 cgaactgatc ctgaacaaac agaattgatg gttctgcatc agcgagaact gacgcaatct
     3061 caggcggagc aagcctgaaa ttgcagacta catagattag ccctgcgttg ggcacgctga
     3121 gtgacgattc aatatactcg aaactattct ggctgaggat ggccacccgc tccccctttt
     3181 ttattccaag atcgtagagg gcgttaccca tcctgtttgt tctgtccttt aactctttat
     3241 acgtgtatgc tcttgtgcca tcaatctcct tcattgctat tttgtcagga tataacatcg
     3301 catttcgatc cagtaaggtt ctgatcgttg agaattgttt ttgttggccc atataacccc
     3361 tcctgttcaa tgtttgtttg ctcctaataa gcaagcaaag tgccatgagc gtaaaaccaa
     3421 gactggcgtg ggtttggaca aatcaaaagg tcttgggtga gccattcttc tgcactatgt
     3481 ttttacaggg tgtattgaaa ataaccacag agttgtatta aaattaaaca gttaattatc
     3541 aggacgtcgg gttgtcaatc acctgcggag agccaatatc gtcataatca cattaaatca
     3601 aaggagagca gcaaaaatcc caaagcggtt gagaaatttc taacctggaa tgcaacgtgc
     3661 atatacttcg agaggaagtg ttgaaaggtt tgatacaatg aatgaaaaga aagggtatga
     3721 actttacaga gacagggcga acaaagaaca tgaggcgagg atgttctggc aaccaagggt
     3781 taatggatgg ttgaggaaga gattgcccgg tgcctccggg ataaagagta agagccttat
     3841 taggaatttt agaaatgccc caatatttcc agaacagaca cctgccgagc gtgcacaccg
     3901 ggagtcggaa aataaattca gagatcttgc agagaaatcg cttgttggca tctatcttat
     3961 tcaggatgat gtttttaagt atgtaaaccc gaagcttgca gagatatttg gctattcaat
     4021 cgacaacctc acgggcaaaa aaagaaagac tccgaaagac ttggtgttac ccgaggactg
     4081 gcctctggtt caggataata ttcggaaaag aatctcaggc gagacagaat cgattcattt
     4141 cgagttcagg gggataaccc ggaaaaaaga gattatttat gtggaggtct atggatcccg
     4201 cactgtgtat caggaaagac ctgcccttat tggtactctt cttgacataa cggggaggaa
     4261 aaaggctcac gagaaattgc agcaaacaga aaaaaaatac cggagcatct ttgaaaatgc
     4321 tgtggagggg atatgccaaa ccacaccgga gggtaagccc atttcagcca accctgcttt
     4381 tgctaagatg cttggctatg attcaccgga tgagtttctg tcatcagttg cggatgttag
     4441 gaatcagatt tatgtgcatc ctgaaatccg cacaaatttt atggagagta tcgaaaggga
     4501 tgggctggtg agaggctttg aatgcgaatt atatcgaaaa gatggaagtc agatattggt
     4561 ctccataagt gcccgcgatg tccgcgatga aaacaaccgt atcctgtatt atgaggggac
     4621 agtagaagac atcacagaaa aaaagaaggt agagcgggaa ttggaaatgc ttcaccagct
     4681 caacacgtca attatcgaac atgccccggt ggcaattttt accattgaca tgcagggaaa
     4741 attcatgagt gtaaatccgg cccttgcaac cctttcagga ttaggacgtg aagccgagga
     4801 aaaactcctg ggctttaact ggcttgagaa ccagtttacg attcggtgtg gccttgcgga
     4861 atatataaga aaaggcctcc aaggtgaacc atttcagctc tgggattttg cattcatgac
     4921 gtacagaggt gacaggaaca tctttataga ttttaaaggt gttcctctca aaggaaagga
     4981 cggagaaatt gaaggtcttc tttgcatcat tgaagagact actgagcgtg taaaaacgag
     5041 agctaaactc atgcaggaag taaaaatggc aaccattgga aggctctcgg cagggatcgc
     5101 ccacgaactc aacaacccac tgggcaccct ggttgcatat gctgaacggg catccaatta
     5161 tcttgaatca atcgacaaca tttttctgag ccaatatgaa tttgaaaaac tcaagggcta
     5221 ccttaagatt attgatgagg aggcattcag atgtaaaagg gttgtgtctg atatcctcag
     5281 cataccgaaa aatgagggac aggagattac acgagtcgat attgagagac tccttgatac
     5341 tatcctcgaa cgattgagcg ctgaaaatcc tggcctgagc attacgaggg aaactatggc
     5401 actgttgccg gtgatacctg gcgacacgaa tattcttcgc cagatctttg ttaatctcat
     5461 aaacaatgca ctggatgctt tagaagggag gaaagatgcg cagttatgca ttcgtaccgc
     5521 gttgagaaac aacatggtag tcgttgaagt tgaagataat ggcatcggga taccagatac
     5581 cattatcgag aagatttttg aacccttttt cacaacgaaa gaatcaaaaa aagggattgg
     5641 tctcggccta tcattatgtt atgactttgt taagggtatg gggggaacca tagaggttca
     5701 cagcggacct gcatctggca ctaattttgt tgtgtcgttg cccatcgatc aagatagaaa
     5761 aaaaagagag gagggcaacc atgattaatg tattaatcgt tgacgatgaa attcgtcttg
     5821 ccgaggcctt tcgtgaacaa ctaactgaag aagggatgta tgtcactgta gcacaccgtg
     5881 cgaaagaggc attatcagca atgaaacgag aaaatttcga tgttgcagtt cttgatatca
     5941 agcttcctga tatggatgga gttgagcttc tgctgaaact gaaacagctg gaaccaacca
     6001 ccgaggtaat catgcttacg ggatatgctt ctgtagcgac ggcaatccgc tctatgaagc
     6061 ttggagcata tgactactta acaaagccct gcaagatgac agaactttca aatgtaatta
     6121 ctaaggccca tgagaagaag gctttgaagg taaagaacat cattctcgaa gaacagcttc
     6181 ataggatagg gggcagcaac gattttgttg gaaacagtaa ggagatggaa aaggtgagag
     6241 gccaaataag catggttgcc ggttcagaca ttccagtgct tattctcggt gaaacaggga
     6301 ctggcaagga gcttgctgca agggcaatcc acgaccttag cccccgtgct ataaaccctt
     6361 ttgtggtggt caactccagt atacttcagg aagccatgct ggaaagcgag ctctttgggt
     6421 acaggaaagg cgctttcacc ggggctgaga cagataaaat gggcctcatt gaaattgccg
     6481 acaggggcac gttctttatc gacgaagtcg gggacatggg aatgagcata caggcgaaat
     6541 tgctgagagt gcttgaaaca ggtattttcc ggaagctcgg ggacaccaaa gaaataaaag
     6601 tggatgtccg ttttattttt gccacaaaca aagatttaaa tgtggctgct gaggagaaac
     6661 aatttcgcag agaccttttt tttcgtatca gcacttttgt tatcaacctt ccaccactca
     6721 ggggcaagcg agaagacata ccgctcctgg tcaggtattt ccttgagaaa tgctccaggg
     6781 gagggaaaaa gaaacatctc tcccgggaag cgatggagct cctcatggca tatgactggc
     6841 cgggcaatgt acgagaactt tcaaatgttg ttgaaagggc agccatcgtg agcaacgata
     6901 gggaagaaat acttgcacag gacctgtcgg aaggtatgtt aaatcctgtt tcacaggtga
     6961 aagaatcaaa agattcgcag attaagggca ggatactgac cctcagggag atggaggcag
     7021 aatatattcg aaatgttttg caatctgtcg ggggtaataa aagcagggca gcacgtcttc
     7081 tgggtatcag caggaagaac ctctatgaca agatcaatgt ttcccattcc tgacctaatt
     7141 tttctcgcta tgtacggttt cttctcttaa tgatataata aaaactactg aatcgagaaa
     7201 ttatccttac caaggtaatc gtttctcagg aacgaacgta catggttttt gggaaaaaga
     7261 aacatattac aatgtttgac gaggttcttg aatcgagttg gaagcttctc catgagggcg
     7321 ttcagagctt ccggagtcca tttcaccatg cagcccttac cactattgat ggagacaaac
     7381 cccgggcgca gcttttcata ctgagagaat tttcagaaaa gaaccggata ttcatatgtc
     7441 actgtgatat cagaggttgc aaggttttac aaatccgaga taatcgaaat gtaagccggt
     7501 tattctatga ttctgagaag tggttgcaag tgcgcctctc atgaaagggg agagctaatt
     7561 ccatgaaaaa ataatgcgtc tgtctattct ttctcctttg gcaagctcat atgggaatgc
     7621 tatacttgga ttaaaacaaa gattacaatg gaaggaataa gaacatgata aaggggggct
     7681 ttgaagaaaa tgaagatgat caataagcat tattgggtag ttggacaagt aaccagggct
     7741 gaggtgatgc ttgtgggcaa tggaggaggc cacatattgg gcatgtttga gagcagaggt
     7801 ttggctcata taggcaatga ggtggttgtt gtgacgtcat gggtccaagg tgatcttcta
     7861 aatggaaatg gcccaatccg aggatatacc cgatacatgt atgaagatgg atctacaatt
     7921 atttctaaga cagaatatac ctgcaagtcg aaccctgaat ccaaggccca tctttacgag
     7981 aatggttatg gtgagttcct ttcaggcagt ggtcggtttt atggaattaa gggtagctcg
     8041 tcctggaagg gaaggcaggt taccccgatt tctgatgaga caaaggggga ttggatcgtt
     8101 gaaggtgata tggtatatac tttaccgcct gagtgacagg aatgaaaaaa caggggggct
     8161 tcaataaact tatgctcggc ggtgtgagtg ggatacatac cacacgtcga atatttcatt
     8221 caaaagaaat tctgtgacac cgggcgggcc aataaacggt tccactgatg cattgaacat
     8281 tttttgcaga tctccaatag cttcattgtc agtaggatct tccataacaa ctgtgagaac
     8341 ccgttttgtt ttatcaagtt gaagagggat aacccgtttt tttcgaaaat aggcatgatt
     8401 cgcttcatca agcaaatttc tgtcaacatg gtccttcctg ggatgcatct tttcaattcc
     8461 aagttgaaca cagagagaat caatgaggac cgccgaggtt atcatcccat ttttaatcaa
     8521 gatttgcccc agcttctccc cgcttttctt ttgctgttgc agggcctcat ccagctgttc
     8581 gtctgatatg atccccattt tattgataag ctctcccaga aaaatttgtt tctggtatcg
     8641 tgaaagaaaa tctcggagta cccgtcccga gatgaaaccc agttccttgc atatttcccc
     8701 aagtggtttg tattccttga gcttcctttg gacagtgagt gcttcttcga gttgcccctc
     8761 atcaagaaac ccgcgtttca cgagaagctg tccaactttc atttcaaggg cataggctgg
     8821 tttagcttgc atgttaaatc ttatcggcaa aaagtaatga tgctttactt tttttaatta
     8881 aaaaattaaa aacatgcgcc ataatcagca tatggcaatt ctttatttca tctggtaatt
     8941 atcagtaacc atctttttca aaattaccca acaaaaggcg gatagacggt gttgtccgtt
     9001 ttagtaaaaa gagcgcagac ttaggaagat tcaaatattt tagataaaaa gtagaatctg
     9061 atggaaaaat ggctcagtat gccaatactt
//
LOCUS       DAOOMC010000114         9069 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdus sp. isolate AMR_MDS_2685
            NODE_8956_length_9069_cov_7.498447, whole genome shotgun sequence.
ACCESSION   DAOOMC010000114 DAOOMC010000000
VERSION     DAOOMC010000114.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864760
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdus sp. (wastewater metagenome)
  ORGANISM  Syntrophorhabdus sp.
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae; Syntrophorhabdus.
REFERENCE   1  (bases 1 to 9069)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9069)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,729
            Genes (coding)                    :: 2,708
            CDSs (with protein)               :: 2,708
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 7 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9069
                     /organism="Syntrophorhabdus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8956_length_9069_cov_7.498447"
                     /isolate="AMR_MDS_2685"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2699756"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..279
                     /locus_tag="PK125_10855"
     CDS             <1..279
                     /locus_tag="PK125_10855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB38643.1"
                     /translation="SNNTTVIALFIEIIITIQRFVKNYHLTGEKRRKGENPCSTLFGC
                     PPSPCEQLLETCIWGLPLNPLPLKTMYTFCKYYGRWVPLIRDFLILHV"
     gene            443..1159
                     /locus_tag="PK125_10860"
     CDS             443..1159
                     /locus_tag="PK125_10860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HPB38644.1"
                     /translation="MTSSDFSRLKWRQTDFRTRRAVVEKLDDQELLAKIAIHDDDYHV
                     RESAVKKLNDPALLAEIARNDKDYHVRRTAVRELDDQALLAELAKNDSDSGVRESAVK
                     KLNDRALLAEIARNDKDYHVRRAAVEELDDQALFSEIAQNDDDYHVRESAVKKLKDRV
                     LLGKIAETDKNSWVRRVAVEKVTDQKLLADIAMNDKDSWVREIAVKKVSDQMLLDELA
                     QKSTDRYIRKAALEKIRKKA"
     gene            complement(1265..1777)
                     /locus_tag="PK125_10865"
     CDS             complement(1265..1777)
                     /locus_tag="PK125_10865"
                     /inference="COORDINATES: protein motif:HMM:NF012365.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB38645.1"
                     /translation="MIKRKTAVVALFLFVSVTCFCSYAFAQAPGGGKDVGVLVAKIIM
                     GISLSLGLAGSAIGLAIAFSALLGGGQENFFKNIAVALMPSTQGIYSIVVLFANMDGF
                     EADSYATAGKAAAFGFAMFFSAWYQGVVCAAGIRSILEGRNTLANAMVSGAMPETYAV
                     FALVMTFIMK"
     gene            complement(1782..3602)
                     /locus_tag="PK125_10870"
     CDS             complement(1782..3602)
                     /locus_tag="PK125_10870"
                     /inference="COORDINATES: protein motif:HMM:NF013647.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033179 - proton-transporting V-type
                     ATPase, V0 domain [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase 116kDa subunit family protein"
                     /protein_id="HPB38646.1"
                     /translation="MAIAKMRRVFIVGLNDEKERTLRVLQDIGVLHVEPFTRLSSAAE
                     EQNTHIAQNVRRIGQIYESLKDYRNPKEKSETFFSGEEILLRAGSILERLQELKNRKQ
                     TLEKLITELSVWGDFDTDYLKALEEQNLFIQRFRAEGVSQSEQVFPEDVFVQTVSHKP
                     ALLFFTIRMGHPVDLPWAVPLRRPEKGLEKVKEEYTSLVTEESKALKELSLLASRIDA
                     LRNVYFATLSEVKFRECLGSLYADELLFGVQGWLPADGEKDFFDKVTSTGLAITISTR
                     DPLENETPPTLFKNNRLIRRIEPLLKLYGFPGYRDLDPSYFFAPFMILFFGICLGDAG
                     YGIVFLVASMWIKKRFGHLSPSLPMVLKLCEAFSISAIIIGLLTGSIFGHWIFPRQWI
                     AVDLDKDHGNPMILFYASLGLGVIHLSLSYVMGIFQSFSRFDRFQKVGLLCVLWGGAC
                     LISQRIWFSDPVSALNQWLHYSGVALVALGVVLTFLFTSDSKKLIVRLGLGLWGIYGL
                     TGLVGDLLSYARLFGLGIATTAIAAVMNDLAGMVHRAAGPVAGSAFAVFILILGHTFN
                     LLLSILGSTVHSARLHFVEAFKSFFQGGGTEYKPFKVERG"
     gene            complement(3643..4257)
                     /locus_tag="PK125_10875"
     CDS             complement(3643..4257)
                     /locus_tag="PK125_10875"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016471 - vacuolar proton-transporting
                     V-type ATPase complex [Evidence IEA];
                     GO_function: GO:0008553 - P-type proton-exporting
                     transporter activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HPB38647.1"
                     /translation="MAERIRLNKVSLREQKQKMAMYERFLPALEARKQQLIMQLAVIR
                     TNILVQRELMETMLADIRSWASLYWDVGKILRYYIAIREIRCVSRNVAGLKIREFKEV
                     IFDDPGYSLFRTPFSFDTILKKTRDAISLREGIKILEEQEQVLLEGFRKTSQRINLYE
                     KRLIPQCREAIRRISVYLQDQQAAAVGVAKVAKRLGEEASYYVD"
     gene            complement(4271..5578)
                     /locus_tag="PK125_10880"
     CDS             complement(4271..5578)
                     /locus_tag="PK125_10880"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF003235.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HPB38648.1"
                     /translation="MIIEHSRITLIQGDIIAVPATGIQYGELAVVKSKRKSSLAQVIR
                     LRGDEVFLQVFAGSRGVATGDQVRFLGHPMQVAFGDAMLGRVFNGSGDPIDGGPNLET
                     LPRLEIGGPSVNPVKRIIPRGFIETRVPMIDVFNPLVESQKLPIFAAAGEPYNQLLSR
                     IGMRADADVVILGGIGMKYDEYLKFRIAFEQAGVLPRTIMFIHTASDPVVERLLVPDM
                     SLAVAEQFGVRGKRVLVLLTDMTAFADAMKEIAISMDQVPSNLGYPGSLYSDLAARYE
                     KAVDFQDAGSITILAVTTMPGDDVTHPVPDNTGYITEGQFYLKNGVIEPFGSLSRLKQ
                     LVIGKVTREDHGYIMNSMIRLFAKSRDVEQKLSMGFEKTAEDERYLRYGQLFYERFMD
                     LRVDIGINEALDLGWKTLKECFSADEVGIKQELIDQYWPGTSG"
     gene            complement(5575..7347)
                     /locus_tag="PK125_10885"
     CDS             complement(5575..7347)
                     /locus_tag="PK125_10885"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF003220.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HPB38649.1"
                     /translation="MAHPINSRVIAVNGPLVTAEVIRDQEVTMNEVAYVMWNDIPLKS
                     EVIRIRGRLVDIQVFESTTGLKVGDQVDFSRELLSATLGPGILGQIYDGLQNPLNMLE
                     NTQGFFLKRGQYVSALDQTKKWDFTPLVAKGDQVKAGHYLGYVPEGIFRHHIMVPLSY
                     AGIGEIVEIAGQGPFTVGETIAVLRNGDSEHFSVTMEQKWPVKMPIRAYNERLLPSRP
                     LLTQCRLIDVFFPLAEGGTACIPGPFGAGKTVLQQIISRYAEADVVLIVACGERAGEV
                     VETIREFPDLEDPKTGKSLMDRTVIICNTSSMPVAAREASIYTGITLGEYFRQIGMKV
                     LLLADSTSRWAQALRESSARMEEIPGEEAFPAYLESRIAALYERAGLVRLYNNETGSL
                     TIVGNVSPAGGNFEEPVTQNTLKVVGAFYGLSRSRSDQRRYPAIDPLISWSLYLRQLE
                     ESLSERHPKWVELVEEAQSLLHRGSEVHQMMLVVGEEGISIDDLVVYLKAEAVDAVCL
                     QQDAFDKVERATSKERQVSDFLLLMEMVHHHFSFESKEQAKKEMTNIQNIFFQMKYAP
                     FQEEAYWAYRKEIESILKKGVKSE"
     gene            complement(7361..8071)
                     /locus_tag="PK125_10890"
     CDS             complement(7361..8071)
                     /locus_tag="PK125_10890"
                     /inference="COORDINATES: protein motif:HMM:NF022415.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2764 family protein"
                     /protein_id="HPB38650.1"
                     /translation="MAKYYFLASCLPPMPISLGEKVALPFEEICGLILRNVEPVDDPL
                     VRCCLHAVDTANTEFFLLGQNIFLPGGGLTRDEIEAKKHLPLFLKKFFEEKDKGIGRG
                     YVYDVLWAEYYAYAYSLAEDLNCRFLIDYLSWEIGLRNSLVELRVRMLGEEAEDFQIL
                     VRAGGYDFSGIISQLKMQQNPLKAEQFLDEERLKRIYHCEGSDPFSRDFILATLEKAR
                     IFSRWERINAIYPVRDII"
     gene            complement(8075..8698)
                     /locus_tag="PK125_10895"
     CDS             complement(8075..8698)
                     /locus_tag="PK125_10895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB38651.1"
                     /translation="MTRTLGDITNELKEKVLSPAKEEAERMLAEARLEADRIILDAKK
                     EASRITEEAKQKADVTVKQMEIDMDTAARNFILLVQERLEKTIVRPVTEDEIESTLSD
                     KDFLSSIIQILIAEFSRVHGKDNKIEILLPSRHKEALEEWFVNKFLQRATKGVVVHFS
                     DKVAFGFKMGFGDTGRYFNFGEGLVEVFSEFCSPRFRKHFFAPDKDS"
BASE COUNT         2611 a         2233 c         1861 g         2364 t
ORIGIN      
        1 tcaaataata ccacggtaat tgcgctattc atagagataa ttattacaat acagaggttt
       61 gtgaaaaact accatttaac aggagaaaaa cgacggaaag gcgagaatcc atgctccacg
      121 ttgtttggtt gcccaccatc accatgtgaa cagttattgg aaacctgcat atggggactt
      181 cccttaaatc cgcttccatt aaaaaccatg tatacatttt gtaaatatta tggtcgatgg
      241 gtgcctttaa tcagggattt cctgatttta cacgtttaaa aaccgtataa aaataagaaa
      301 agcatcattt ctgttgacat cagtgacagg tagtcgcaaa atacaaaagg tgcatctggc
      361 agcaggttaa ctcatgtagg attcttgaga ccaaaaattg cacgtcagaa ccattgtgta
      421 caagacttaa ggaggctccc aaatgacttc gagcgatttt tctcgattga agtggagaca
      481 aaccgacttc cgcacacgca gagctgttgt ggaaaaactc gatgatcagg agttgctggc
      541 aaaaatagca attcatgacg atgattatca tgtgcgtgag tctgcggtaa agaagttaaa
      601 tgacccggct ctactggctg aaatagcccg gaacgataag gattaccatg tgcgcagaac
      661 tgcagtgagg gaacttgatg accaggcact gctggcagaa ttggcaaaaa acgacagcga
      721 ctccggggtg cgtgaatctg cggtaaagaa gttaaatgac cgggccctgc ttgcagaaat
      781 agcccggaac gataaggatt accatgtgcg cagagcggca gtggaggagc ttgatgacca
      841 ggcactgttt tctgaaatcg cgcagaatga cgatgattat catgtgcgtg aatctgcggt
      901 aaagaagtta aaagaccggg tcctgctagg gaaaatagcg gagaccgata aaaactcttg
      961 ggtacggagg gttgcagtgg agaaagtcac tgaccagaaa ctgcttgcag atatcgcgat
     1021 gaatgataaa gactcgtggg tacgagagat tgctgtgaaa aaggttagcg accaaatgtt
     1081 gctggatgag ttggcccaaa aaagcacaga cagatatata cgcaaggctg ctctggagaa
     1141 gatcaggaag aaggcataac ttccggaaaa gcacgtcaaa atgtaaatta aacttgaatt
     1201 tttcagtcca gatcaaataa gggcaggact gaagtgaccc gcgtttgtaa cttgaccagc
     1261 tttcttactt cattataaat gtcatgacca gagcaaaaac agcatacgtc tctggcatag
     1321 ccccagaaac catagcattg gcaagtgtgt tcctcccctc aagaatactt ctgatgcctg
     1381 ccgcgcatac aacgccctgg taccaggcgc tgaaaaacat cgcgaagcca aaggcggctg
     1441 cttttcctgc agtcgcataa gaatcggctt caaaaccatc catgtttgcg aatagtacaa
     1501 ctatggagta aataccctgt gtggaaggca taagcgcaac agctatgttc ttaaaaaagt
     1561 tctcctgccc tccgccaaga agagcagaga aagcaatcgc aagaccgatg gcagaacctg
     1621 ccaacccgag tgacaggctg atgcccatga taatcttggc tacaagaaca cccacatctt
     1681 taccaccacc cggcgcctgt gcaaaggcat aggaacagaa acaagtcaca ctgacaaata
     1741 agaataaggc taccacggct gtctttcttt ttatcatatc gttaacccct ttccactttg
     1801 aatggtttat attcagtgcc tccaccctgg aaaaaactct taaaggcctc aacgaaatgg
     1861 agccgggcag aatggactgt gcttccgaga atagagagaa gaagattgaa ggtatgtccc
     1921 aggatgagta taaaaacagc gaatgcactt cctgcaaccg gaccagcagc ccggtgaacc
     1981 atccccgcga gatcattcat cacagcagct atggctgtcg ttgctatacc gagcccaaac
     2041 aatcgtgcgt atgaaagcag gtcacccaca agacccgtta atccataaat gccccatagg
     2101 ccaaggccaa gccttacaat caatttctta gaatctgaag taaaaagaaa tgtgaggacc
     2161 accccaaggg caaccagggc aactccactg taatgcaacc attggttcaa cgcagaaaca
     2221 ggatccgaaa accagatgcg ttgacttatc agacaggcac caccccataa gacacacagc
     2281 aacccgacct tttgaaaacg atcgaacctt gaaaaagatt gaaaaattcc cataacatat
     2341 gaaagggaca ggtgaattac acccagacca aggctcgcat agaagagaat cattgggtta
     2401 ccatgatcct tgtcaagatc cactgcaatc cactgtcgag gaaatatcca gtggccgaaa
     2461 atcgatcctg tcagaagccc tatgatgatt gcagagatcg agaaggcttc acacagcttc
     2521 aaaaccatgg ggagagacgg ggagagatgg ccgaatcttt tcttaatcca catagatgca
     2581 accaggaata ctattccata accagcatct cccagacaga ttccgaaaaa cagaatcatg
     2641 aaaggtgcaa aaaaatagga aggatcaagg tctcgatagc cagggaagcc ataaagctta
     2701 agcaaaggct caatgcgtct gatcaatcgg ttatttttaa acaatgttgg tggggtctca
     2761 ttttcaaggg gatcccttgt gctgatggta atggccagac cggttgatgt aactttgtca
     2821 aaaaaatcct tctccccatc tgccggcaac caaccctgca caccaaaaag taactcgtca
     2881 gcgtaaaggc tgcccagaca ttctctaaat ttcacctcac tgagtgtagc aaaatataca
     2941 ttcctaagcg catcaatccg cgaggccaac aaagagagct ccttgagggc ctttgattct
     3001 tctgttacaa gggaggtata ctcctctttt actttttcta aacctttttc cgggcgacgc
     3061 aaaggaacag cccatggcag gtcaaccgga tggcccatac ggattgtgaa gaacagcagg
     3121 gccggtttat gagacacagt ctgtacaaat acatcttcgg gaaaaacctg ttctgattga
     3181 gataccccct cggctctgaa ccgctgaata aaaagatttt gttcttccag ggcttttaga
     3241 tagtcagtat caaaatctcc ccatacactc agttctgtaa tgagtttctc aagtgtctgc
     3301 tttctgtttt ttaattcctg gagtcgttcg agaatactcc ctgctcgaag aaggatttcc
     3361 tcgcctgaga aaaaagtttc acttttctct ttaggattcc ggtaatcctt caaagattca
     3421 taaatctgac caattcttct aacgttctgt gcaatatgag tattctgttc ctctgctgcg
     3481 ctgctcagtc tcgtgaaagg ttctacatgc aaaactccaa tatcctggag aacacgtaat
     3541 gttctttctt tttcatcgtt aaggcctaca ataaagactc ttctcatttt ggcaattgcc
     3601 aaacagtcac cttcctataa caagtttctt cctgagtaaa ccctagtcaa cataatacga
     3661 cgcctcttcg cccaaccttt tcgctacttt ggcaacaccc accgctgctg cctgctgatc
     3721 ctgaaggtaa accgaaatcc tccgaatggc ctccctgcac tgagggataa gccttttttc
     3781 atacaggttg atgcgttggc ttgtctttct gaaaccttca aggagaacct gctcctgttc
     3841 ttcaaggatt ttaatacctt cacggagtga gattgcatct cttgtttttt ttagtatagt
     3901 atcaaaacta aagggcgttc ggaaaagact gtaccccgga tcgtcaaata tgacctcttt
     3961 gaattctctg attttcaatc ctgctacatt ccttgaaaca caccgaatct cccgaatagc
     4021 aatataatac cttaatatct tgccaacatc ccaatagaga gaagcccatg aacgtatatc
     4081 tgcaagcatt gtctccatga gttcccgctg aaccagaatg tttgttcgaa ttaccgctaa
     4141 ctgcatgatg agttgctgct tcctcgcctc aagggcagga agaaatcgct catacatggc
     4201 cattttctgt ttctgctccc tgagtgatac cttattgagc ctgatccgtt ctgccattga
     4261 ttattgcacc tcaacccgaa gttccgggcc agtattgatc aatgagctcc tgtttaatgc
     4321 caacttcatc agccgaaaaa cactctttta gcgtcttcca tccaagatcg agggcttcat
     4381 taatgcctat atctaccctg agatccatga aacgttcata aaacaattga ccatacctga
     4441 gataccgttc atcttcggct gttttttcaa aacccataga aagtttttgt tccacatccc
     4501 ggctttttgc aaatagccgt atcatgctgt tcatgatata accatgatcc tctcgggtaa
     4561 cctttccgat gactaactgt ttcaaccgtg ataatgatcc aaaaggctca attacaccat
     4621 ttttcaggta gaattggccc tctgtaatat accctgtatt gtcaggaaca gggtgggtca
     4681 catcatcgcc cggcatagtg gtcacagcaa gaatagttat actccccgca tcctgaaaat
     4741 cgacggcctt ttcatagcgt gctgccaggt cactgtaaag agaaccggga tagccgagat
     4801 ttgaaggaac ctggtccatg gaaatggcaa tctctttcat ggcatcagca aaagctgtca
     4861 tatctgtaag gagaaccagg actcgctttc ctctgacacc aaattgctct gctacggcaa
     4921 gagacatgtc tggaacaagg agacgctcca caacagggtc agatgcggta tgaatgaaca
     4981 ttattgttct gggaagaaca ccggcctgtt caaaagcaat acgaaatttc agatattcat
     5041 catacttcat ccctataccg ccgaggataa caacatctgc atcagcgcgc attccaatgc
     5101 gcgaaaggag ctgattatat ggttccccag ccgctgcaaa aataggaagc ttctggcttt
     5161 ccaccagagg gttaaataca tcaatcattg gtacacgggt ttcaataaag ccacgaggaa
     5221 tgatgcgctt aaccggattg actgatggcc ctccaatttc caggcgaggc agggtttcca
     5281 ggtttggacc gccatctatg ggatctcctg aaccgttgaa gacccttccg agcatggcat
     5341 ctccaaaggc aacctgcatg ggatgtccta aaaaacgaac ctgatcacca gttgcaacac
     5401 ctcttgatcc ggcaaatacc tggaggaaaa cctcatcccc cctcaaacgt ataacctggg
     5461 cgagagagga ttttctttta ctctttacta ctgctagctc tccatactga atgcctgttg
     5521 ctggaaccgc tatgatgtct ccctggatta atgttatccg agagtgttca ataatcattc
     5581 agacttcacc cctttcttga gaatagactc tatttccttc ctgtaagccc aataagcctc
     5641 ctcttgaaag ggggcatact tcatctgaaa aaagatgttc tgaatgttgg tcatctcttt
     5701 tttcgcctgt tctttactct caaaagaaaa gtgatggtgc accatctcca taagaagaag
     5761 gaaatcagaa acctgcctct ccttcgaagt ggccctttcc accttatcaa aagcatcctg
     5821 ttggagacat acagcatcta cagcctctgc cttcagatac acaaccagat catcgatact
     5881 gataccctcc tccccaacaa caagcatcat ttgatggacc tcgcttccgc ggtgaagaag
     5941 tgactgtgcc tcttctacaa gctcgaccca cttcggatgg cgttcgctta atgactcctc
     6001 cagttgcctc aggtaaaggc tccacgagat tagtgggtca atggccggat agcgcctctg
     6061 atcagaacgg gaacgcgaca atccatagaa agcacccact acctttaagg tgttctgggt
     6121 tactggctcc tcaaaatttc caccagccgg tgatacattt cccacaattg tgagtgatcc
     6181 tgtttcatta ttatataatc ttaccagtcc ggctcgttca taaagtgcag ctatgcggct
     6241 ttcaagatac gctggaaatg cctcttctcc cggtatttcc tccattcttg ccgatgactc
     6301 cctcagcgct tgagcccatc tcgacgtgct atcagcgagg agcaatactt tcatgcctat
     6361 ttgacggaaa tattctccga gagtaatacc tgtatatata gaggcttcac gggctgcaac
     6421 cggcattgag gatgtgttgc aaatgatgac agtacggtcc atgagagatt tccctgtttt
     6481 aggatcctcc agatccggaa attcccggat agtctccacc acctcgccgg ctctctcgcc
     6541 acaggcaaca ataaggacaa catcagcctc agcgtatcga gagataatct gttgcaaaac
     6601 tgttttgcct gccccgaaag gtcccgggat acacgccgtt cctccttctg cgagaggaaa
     6661 aaacacatct atgaggcggc actgtgtaag aagggggcga ctggggagta gccgctcatt
     6721 gtaagcccga atgggcatct tgacaggcca tttttgttcc atagtaaccg agaagtgctc
     6781 actgtccccg ttcctgagca ctgcaatggt ttcaccaact gtgaatggac cttgtccggc
     6841 tatttctaca atttccccta tgccagcata agagaggggg accataatat ggtgtcgaaa
     6901 aatgccctcc ggcacgtatc caagataatg accagccttc acctgatcac ctttggcaac
     6961 taacggggta aaatcccact tcttagtttg atcgagggcg ctcacatatt gtcccctttt
     7021 tagaaaaaac ccttgggtat tttcaagcat attcaaaggg ttttgcaggc catcataaat
     7081 ttgtcccaga attcccggtc ccaaggttgc agacaggagt tccctggaaa aatcaacttg
     7141 atctcccacc tttagccctg tggtactctc aaaaacctgt atgtctacca gccgcccacg
     7201 aatccgtata acttctgatt tgagaggtat gtcattccac attacgtagg caacttcatt
     7261 catggtgact tcctggtctc taatcacctc agcagtcaca agaggaccat ttaccgcaat
     7321 tactctgcta tttatcgggt gcgccaatca caacctccct ttagatgatg tcacgaaccg
     7381 gatagatggc atttattctc tcccatcgac tgaagattcg cgccttttcg agcgttgcaa
     7441 ggataaaatc ccgtgaaaaa gggtctgaac cctcacaatg ataaattctc ttgagtcgct
     7501 cttcatccag aaactgttcg gccttcaaag ggttttgctg catttttaat tgtgaaatga
     7561 ttcctgaaaa atcataccca ccggctcgaa ccaggatttg aaaatcctca gcttcttcgc
     7621 caagcatcct cactctcaac tccacaagac tattcctcag accgatttcc cacgacaaat
     7681 aatcgatgag aaaccggcag ttcaaatcct ctgcaagaga ataagcgtaa gcataatatt
     7741 cggcccatag tacgtcataa acatagccac ggccaatgcc cttatccttt tcctcaaaaa
     7801 acttcttcag aaataatggt aaatgcttct tcgcttctat ctcatcacgg gtaagaccac
     7861 ctccgggcag aaaaatattc tgccctaaca aaaaaaattc tgtgttggct gtatcgactg
     7921 catgaagaca acaccgaact aaaggatcat ccacaggctc aacattgcga agaattaacc
     7981 cacaaatttc ttcgaatggc aatgcaacct tttcgccgag agaaattggc atgggcggaa
     8041 gacaactcgc caaaaaatag tattttgcca cgtctcagga gtccttgtct ggtgcaaaaa
     8101 aatgttttcg gaatcggggt gaacagaatt cagaaaaaac ctccacgagg ccttccccga
     8161 aattaaagta tctgccagta tcgccaaaac ccattttaaa tccaaaagca accttgtcac
     8221 tgaaatgcac cacaacacct ttagtagccc gctgtaaaaa tttgttgaca aaccattcct
     8281 caagcgcctc tttatgcctt gagggcagaa ggatttctat tttattgtcc tttccatgaa
     8341 cccttgaaaa ttcagctatc aaaatctgaa ttattgaact taaaaaatct ttatcactaa
     8401 gggttgactc aatctcatct tcagttacag gcctcacaat agtcttttca agacgctcct
     8461 ggactaaaag gatgaagttt ctggctgcag tatccatatc aatttccatc tgcttgaccg
     8521 taacatccgc cttttgtttg gcctcttctg ttatacggga agcctctttt tttgcatcca
     8581 gaataatcct gtcagcctct aaacgagcct ctgccaacat tctctcagct tcttcttttg
     8641 ctggagaaag aaccttttct ttcagttcat ttgtgatatc acccagagtc cgtgtcatgg
     8701 ccatctcccc tgtctttgtc cgtgttcagc cggtggtcat gatgcgcggc aaagaccttc
     8761 aattacgact cgtgttctct tgatcttatt cccatatgac ttgcttcggt ttatataatg
     8821 ccattttaaa cagcttaaaa attatattaa ctgtatttta cttttttttg caagtacatt
     8881 tggacgcata ttgccagaga aaatttgtat aaactccctc agcccacgat gagcatttca
     8941 atcttatgat ctttaatgcg gaaggtccat gtatgacctt ccaggttcag gtgacgggat
     9001 gaagaacagg aagagaaaat ggaagtccat atcaatactt ccagatgaac tggatatggt
     9061 cggggttgt
//
LOCUS       DAOOMC010000115         8884 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdus sp. isolate AMR_MDS_2685
            NODE_9206_length_8884_cov_6.531657, whole genome shotgun sequence.
ACCESSION   DAOOMC010000115 DAOOMC010000000
VERSION     DAOOMC010000115.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864760
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdus sp. (wastewater metagenome)
  ORGANISM  Syntrophorhabdus sp.
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae; Syntrophorhabdus.
REFERENCE   1  (bases 1 to 8884)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8884)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,729
            Genes (coding)                    :: 2,708
            CDSs (with protein)               :: 2,708
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 7 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8884
                     /organism="Syntrophorhabdus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9206_length_8884_cov_6.531657"
                     /isolate="AMR_MDS_2685"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2699756"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..839)
                     /locus_tag="PK125_10900"
     CDS             complement(<1..839)
                     /locus_tag="PK125_10900"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPB38652.1"
                     /translation="MLHYLLKRLAFMIPMLLGITLISFAVIHLAPGEPDIVGAEMNPR
                     VTKEIRERIRNIYGLDKPIHIQYWMWFKRIVKFDFGISFAPDRRPVIDKIKERLPITI
                     SINILSMVLIFLIGIPIGVYCAQHKDTISDKFLTSFVFAGYAMPTFWLALLLMMFFGV
                     YLELLPISGLKSFNYSEMTPLEKLWDLMSHLILPIGISAFGGLAGISRYMKNSLLEVL
                     RQEYITTARAKGLPEHIVIRKHALRNALLPVITILGLSVPGLIGGSVIFESIFSIPGM
                     GQL"
     gene            complement(820..2448)
                     /locus_tag="PK125_10905"
     CDS             complement(820..2448)
                     /locus_tag="PK125_10905"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
                     /protein_id="HPB38653.1"
                     /translation="MKKVFILIIIAAGLASCGKAREATFYDDPGKPAYGDTIITASIG
                     EASNLIPILAGDAASHDISGHVYNGLVKYDKNLNIVGVLAKSWEISTDNLSIIFHLRK
                     DVKWQDGTPFTAHDVLYTYKIIVDPKTPTPYSGDFKPVKEAKVIDDYTFKVTYEKPFA
                     PALQSWSVGMLPRHLLEGTDITTSHLIRKPIGTGPYIFKEWISGDRIVLTANNNYFEG
                     KPFIERRITRVIPDLATMFLELKRYTIDMMGLTPLQALKQTDYPGFKREFSKYRYLGF
                     SYVYLGYNLKHKVFGDRRVRKAIAHAINKQEIIDGILLGQGVEATGPYKPDMWAYNGN
                     VRRYEHSKEKALALLHEAGFEKREDGILVKDGKPFEFTILVNQGNDVRIRTAELIQKR
                     LRDVGIIVKIRTIEWATFVNEFIDKRNFEAVILGWSFGPEPDLFDVWHSSKQGPKEFN
                     FISFENKEVDELIMKARHTMDQEERRKCYFRIQEILADEQPYTFLFIPYSLVAVHKRI
                     KGIEPAPAGITYNNTEWYVPMEQMRYSSRATLSP"
     gene            2585..3493
                     /locus_tag="PK125_10910"
     CDS             2585..3493
                     /locus_tag="PK125_10910"
                     /inference="COORDINATES: protein motif:HMM:NF012403.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HPB38654.1"
                     /translation="MQLTPYHAKYFAYELTKRCSSNSIGKLAASLSDAQVDLNPHQVE
                     AALFAFQSPLSKGAILADEVGLGKTIEAGILLSQKWAERKRKLLIITPSSLRKQWNQE
                     LIDKFFLPSMILETKPFNHEIKKGNLNPFNQSEIVICSFHFARTKEAYIKQIDWDLVV
                     IDEAHHLRNVYKPTNRIANTIKAAVTHCPKLLLTATPLQNSLLELYGLVSVIDDYAFG
                     DLKSFKNQFSRLMDKGEDLLTALERGFAQVEGKGAANQRVYQLLSEKFRLFEGVFGAS
                     DEVLGTIESGVDFKKHFAQIYLECRT"
     gene            3735..3905
                     /locus_tag="PK125_10915"
     CDS             3735..3905
                     /locus_tag="PK125_10915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB38655.1"
                     /translation="MLWMNPFSHEKIHSEPYKGTPENIIVNELTNYPEIDYDDQTEFL
                     FKLARKFQTIEI"
     gene            4533..4970
                     /locus_tag="PK125_10920"
     CDS             4533..4970
                     /locus_tag="PK125_10920"
                     /inference="COORDINATES: protein motif:HMM:NF024675.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HPB38656.1"
                     /translation="MARVKLSEKPRYEFCYPITLQPRDINYGGHLGVDSLISIIHTAR
                     AYIFKSGGISETDLGDGRTGIIMTDVEINLKAEGFMFDNLEVFTHVGELTKKGFRFFH
                     KISRDDSTLALAEMGFLTIDYDTRKVTLVPDACIRALGLLQRT"
     gene            complement(5035..5817)
                     /locus_tag="PK125_10925"
     CDS             complement(5035..5817)
                     /locus_tag="PK125_10925"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011996181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HPB38657.1"
                     /translation="MLLVIDIGNTNIVFGVYDDSKLLNHWRLSTVIQKTVDEYAILLN
                     SLLYIEKIKLSEIDAAIISCVVPPLLIPFDILCRKYVGIRPILVGPGIKTGMPILYEN
                     PQEVGADRIVNAVSAYEKYKRALILVDFGTATTFDYITPRGEYAGGAIAPGIMISLEA
                     LFERASKLPRVELVKPKTITGKNTIHAMQAGIVYGYVGLVDGIVNRMKEEVKTDPYVI
                     GTGGLASLMYRESSSINEVDEFITLNGLKILYEKNKDHHKFK"
     gene            complement(5890..6855)
                     /locus_tag="PK125_10930"
     CDS             complement(5890..6855)
                     /locus_tag="PK125_10930"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HPB38658.1"
                     /translation="MVRSKEILPYLRNTHDYVSGDHMSNIMGISRTAIWKYINHLEQL
                     GYRIEKLKGKGYRLIASPDKLYAWEIDQYCKTNVIGKKIIYKDELDSTNALAFKLAIG
                     GEAEGTCIIAETQDSGKGRLGRKWFSPAGKNLYISIVLRPQIPPSNVYPITFLSSLAV
                     YDTIQSLTKVEPALKWPNDVLVNGRKICGTLLELSTEADMIRFVIVGIGLNINMEENE
                     IDEAIKNKATSFLMLTKKPFERSMVCGILLTNIERYYSAFIEKGPDEICSFWEERAKI
                     KGKYLEIIQMGETYKGISEGIDKDGGILLNIDGKTRKIIAGDVSF"
     gene            complement(6848..>8884)
                     /gene="fusA"
                     /locus_tag="PK125_10935"
     CDS             complement(6848..>8884)
                     /gene="fusA"
                     /locus_tag="PK125_10935"
                     /inference="COORDINATES: protein motif:HMM:NF009381.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HPB38659.1"
                     /translation="KEILRLRKIRNIGIAAHIDAGKTTVTERILYYAGKTHRIGEVHE
                     GTTIMDYLPQEQERGITITSAVTTLYWEDHEIQLIDTPGHVDFTIEVERSLRVLDGMV
                     AVFCGVGGVEPQSETVWHQADKYRVPRIAFINKLDRVGSDFFHVMDMLVEKFGANPIP
                     LQIPLGREDEFFGVIDLITMKALAWSEDDLGATFNLISIPEEYEQLARSQREKMLEKC
                     SLLDDQFMELYLEGKEIDDAMIHETLRQGTIALKCVPVLCGTALRNKGIQPLLDSVVR
                     YLPSPLDIPPVQGINPQTGQMEQREVSDEEDLAALAFKIVLDEGRKLTYIRIYSGTIK
                     VGDDIYNVNLGKREKVSRIFRMHANHRDRIEEARTGAIVGIVGLKETTTGQTLTKAKP
                     LLLEPIEVYQPVISIAVEPKRNVDQDKLLLALQRISDEDPTFVLKTDEDAYQTVVSGM
                     GELHLDVIVRRIKDEFGIGLHVGKPQVVYKEAIEDEASIEHAFEKIINNVTCKGWVSL
                     LVQPNTRGGGIQIVNSISEESPVHPYLMAIEEGVLEASNMGILKGFPVTDVKVSIRDA
                     TFNSPEFARLTLKMAAYEAFRDACQLAKPILLVPIMSLTVTVPNEFLGDIISDLNARK
                     SQVTNVTTSDKITIVQANAPLTKMFGYSTDVRSLSQGRASFTMYFSHYDKIENG"
BASE COUNT         2349 a         1986 c         1965 g         2584 t
ORIGIN      
        1 aaaagctgcc ccatccctgg aatggagaat atagattcaa aaatgacact tcctccgata
       61 aggcccggga cagataaccc cagtattgtt ataaccggta acaaggcatt gcgaagtgcg
      121 tgttttctga tgacaatatg ctcaggtagg cccttggcgc gggcagtggt tatatactcc
      181 tgtctcaaca cctcgagaag gctgtttttc atgtaacgcg atatcccggc aaggcctcca
      241 aaagcagaga ttcctattgg gaggatcaga tggctcataa ggtcccagag cttctcaagg
      301 ggcgtcattt cactataatt aaatgatttc agccctgata ttgggagtaa ttcaaggtaa
      361 accccgaaga acatcatgag gagtagagcg agccagaagg ttggcatggc atagcctgca
      421 aaaacaaagg atgtaagaaa tttgtcgctt attgtgtctt tatgctgggc acaataaacc
      481 cctatgggaa tgccaataag aaagataagg accatggaaa ggatgtttat tgatatggtt
      541 atgggaaggc gctccttaat tttatcgatg acaggccgcc tgtccggggc aaaggagatg
      601 ccgaagtcga actttacgat ccgtttgaac cacatccagt actggatatg gataggcttg
      661 tctagtccat agatgttcct gatccgttcc cgtatctcct tcgttaccct ggggttcatc
      721 tctgccccaa caatgtctgg ttctccgggg gcgagatgga tcactgcaaa tgatatgagg
      781 gtaatgccta aaagcatggg gatcataaac gccagtcgtt taaggagata gtgtagcacg
      841 ggagctatac ctcatctgtt ccatgggaac ataccactcg gtgttgttgt atgtgatgcc
      901 agccggagca ggttctatcc ctttgattcg cttatggact gcaacaaggg aataggggat
      961 gaagagaaat gtatacggct gctcgtcagc aaggatctcc tgaatacgga aataacattt
     1021 tctcctttcc tcttgatcca ttgtatgacg ggccttcatt ataagttcgt caacctcttt
     1081 gttttcaaaa gaaatgaaat tgaattcctt tggaccttgt ttcgatgaat gccacacatc
     1141 gaacagatct ggctccgggc cgaagctcca gccaaggatc acagcctcaa aatttcgttt
     1201 gtcgatgaac tcgttcacaa aggtggccca ttcaatggtc ctgatcttca cgatgatgcc
     1261 cacatctctg agccgtttct ggatcagctc tgctgttctg atgcgcacat cgttgccctg
     1321 gttgacgagg atagtaaact caaagggttt tccatccttg acgaggatgc cgtcctcgcg
     1381 tttctcaaat cctgcttcat ggagaagggc aagggctttt tctttgctgt gctcgtaacg
     1441 tcttacattt ccgttatacg cccacatgtc cggtttgtac gggccggttg cctccacacc
     1501 ctgtccgagg agtatgccgt caataatctc ctgtttgttg atggcgtgcg caattgcctt
     1561 cctcacccgc ctatctccaa ataccttgtg cttaaggtta taaccgagat aaacatatga
     1621 gaagccaaga tacctgtatt tgctaaattc ccttttaaag cctggatagt cggtctgttt
     1681 cagggcctgg agaggggtga gccccatcat gtcgatggta taccgtttca gttcgagaaa
     1741 catggtggca aggtcaggga tgactctggt aatccggcgt tcaataaatg gtttcccttc
     1801 aaaataattg ttatttgccg taaggacgat acggtcgccg gaaatccact ccttgaatat
     1861 gtaagggcct gttcctatcg gctttcgtat caggtgggat gtggtgatat ccgtaccctc
     1921 gaggagatga cgaggtagca ttccaacgga ccagctttgc agggcaggcg caaagggctt
     1981 ttcgtaggtt accttgaacg tgtaatcatc gattaccttt gcttccttga ccggcttgaa
     2041 atcgccagag tagggggtgg gcgtcttggg gtctacaata attttatagg tatagagaac
     2101 gtcatgggct gtgaacggtg ttccgtcctg ccacttcaca tcctttctca aatgaaagat
     2161 aatagaaagg ttatccgtag atatttccca tgattttgca aggaccccaa cgatgttgag
     2221 gttcttatcg tatttcacga ggccattgta aacgtggcct gatatatcat gggacgcagc
     2281 atcacctgca aggataggga tgaggtttga cgcttctcca attgatgcgg tgatgatggt
     2341 gtcgccgtat gcaggctttc ccggatcgtc atagaatgtc gcttccctgg cctttccgca
     2401 tgatgcaaga ccagcagcta tgataatgag aatgaatacc tttttcattg tttgtctggc
     2461 cgttattcta acagggggca acagcaaagt aaaggcacac aataaatccc agaaagcttg
     2521 tttttcatga ttttttcttg tcttctcatt ggttaggcgc taaaattttc tcacacgcgc
     2581 taacatgcaa ttaacgcctt accatgcgaa gtattttgcc tatgagctta ccaaacgatg
     2641 ctcgtcgaat agcatcggca agttagctgc atcactatca gacgcccagg tagaccttaa
     2701 tccccatcag gtggaagcag cgctgtttgc ctttcagtcg ccactttcga aaggcgctat
     2761 ccttgctgac gaggttggcc tcggaaagac tatcgaagcc ggcatccttc tttctcagaa
     2821 gtgggctgaa cggaaacgca agcttctcat catcaccccg tcaagtcttc gcaagcaatg
     2881 gaatcaggag cttattgaca agttttttct tccttccatg atcctggaaa caaaaccgtt
     2941 caaccatgag attaaaaagg gcaacctgaa ccctttcaat cagagcgaga tcgtcatttg
     3001 ttcatttcac ttcgcccgaa ccaaagaggc ttacataaag cagattgatt gggacttggt
     3061 ggtcatagac gaagcgcacc acttgagaaa tgtgtacaag cccaccaaca ggattgccaa
     3121 tacaataaag gccgctgtca cccattgtcc gaagcttctc ctcaccgcta ctcctcttca
     3181 gaactcactt ttggaactct atggtcttgt gagtgttatc gatgactatg cctttggcga
     3241 cttaaaaagc ttcaagaacc aattttcaag actcatggat aaaggagaag atttactcac
     3301 cgctcttgag cggggatttg ctcaggtaga agggaagggt gctgccaacc aaagggttta
     3361 tcagctcctg tcagagaagt tccggctttt cgaaggcgta ttcggtgcaa gcgatgaggt
     3421 tctcgggacg atcgaatcag gggtcgattt caaaaagcat tttgcccaga tttacctgga
     3481 atgtcgtaca taagaagaga tacaaaatgc attcgataca ttgcaaaaaa gagatggaaa
     3541 cccagatcga tgaaaccatg aacattacca ggcagaagct ccttgaaaac ttcgacgacg
     3601 aagtcgcgat aagttgaggg tgaatctcaa aaaaaagcaa ggagtatctt tcccggtatg
     3661 agaactggct ataggagctt acaaaatatt atcttgggcc ctatgctgat ttctcatcag
     3721 atgataactc ctttatgctt tggatgaacc ctttttccca cgagaagata cattctgagc
     3781 catacaaagg taccccagaa aacatcattg tgaatgaatt gaccaactat cctgagatcg
     3841 attacgacga ccagaccgaa tttctcttca agctggcacg gaagtttcaa acaatagaaa
     3901 tatagatctt tcacaaaact ctatcacatt tcatatttta tactttagac tcacaatgac
     3961 atgatacagc cgctccatgt ccgggcattt agaacaaatt caagaaaaag taacggctac
     4021 caaatcgaaa gacgtcaccg gtaactccat ctgagtcatc gcttcttgcg aaaaatagca
     4081 tgatcacatt cgaaatactg tggaatgaga ccccgttttt agtattatgc caaaaaaaga
     4141 ggaaaaaagc tccagggcgg ttcttggatt ttatatgttt gttttgatat tttcgagaag
     4201 atcggtcagg ttgcacgcaa aggtggcagt ttttctatgt tcaataaagg gtcgatttct
     4261 catgtttgct gataattgag catagaactg ggtaatattc tgtgagcgct gatcttagtc
     4321 tacctatagt cctcgccggt gtcttcattg ggagaagcca agtctgtgag cacctcttcg
     4381 agatattcag ttctaaagaa atatgccttt ccttgatcgt gtcttgcttt ttcttttcta
     4441 ttatcttatt atccgaagtt atatgataag acattttttc tgatttaaaa ttgattgttt
     4501 ttttacgtga atcattttat gtataatgaa agatggcacg ggtaaaatta tcagagaagc
     4561 cccggtatga gttttgttac ccaattaccc tccagcccag ggacatcaac tacggcgggc
     4621 atctgggcgt agactcactc atatccataa ttcacaccgc ccgtgcttac atctttaaat
     4681 ccggtggcat aagcgagact gacctcggtg atggccgaac gggaatcatc atgacagacg
     4741 tggagattaa cctcaaggca gaaggattta tgtttgataa tcttgaagtc tttacgcatg
     4801 ttggagagtt gacaaaaaaa ggttttcgtt tttttcataa gattagtcgt gacgattcaa
     4861 ccctggcatt ggcggaaatg ggttttctta ccattgacta tgacacaagg aaagtaacct
     4921 tggttcctga cgcgtgtatc cgcgccctcg ggttgcttca gcgcacctaa taggcgaaga
     4981 ttttcctgta tagttatggg agaaaaacca tttcgcggca ctgtaggcgc atatttattt
     5041 gaacttatgg tggtccttgt tcttctcgta aaggatcttc aggccattta gcgtgatgaa
     5101 ttcatcaact tcattgatag atgaagattc ccggtacatg aggctggcga ggcctcctgt
     5161 acctataacg taagggtctg tttttacctc ctctttcatg cggttcacga taccgtcaac
     5221 aagcccgaca taaccataga caatgccggc ttgcatggcg tggatcgtgt tttttcccgt
     5281 aatggttttt ggttttacaa gttcaactct tggaagtttg gatgcgcgtt caaaaagcgc
     5341 ttccagagag atcataatgc cgggcgcaat agctccgcct gcatactctc ccctgggagt
     5401 tatgtagtcg aaagttgttg cagtcccgaa gtcaacaagg attagagctc ttttatattt
     5461 ttcataagct gagaccgcat tgacaatcct atcggcacct acttcttggg ggttctcata
     5521 aaggataggc atgcctgttt tgatgcctgg cccaacaagt atgggtctta tgccgacata
     5581 tttcctgcac agtatatcga aggggataag aaggggaggc acaacgcatg aaataatggc
     5641 agcatcaatt tcacttaact ttattttctc aatgtagagc agactattta aaagaattgc
     5701 atactcatcc actgtctttt gaataacagt gctcagcctc caatgattaa ggagtttgct
     5761 gtcgtcatat actccgaata ctatgttcgt gtttccaata tcaatcacta acaacatggc
     5821 aaagttcctt ggagatctgt atgtcgtata ttgtatgtga cttgtcctgg cctaaaggcc
     5881 aggattttct caaaaactaa cgtctcctgc gatgatcttt cttgtttttc catcgatatt
     5941 aaggagaata cccccgtctt tgtctatacc ttctgaaatt cccttgtatg tctcacccat
     6001 ttggattatt tcaagatatt ttcctttaat ttttgccctt tcttcccaga atgaacatat
     6061 ctcgtcaggg cctttttcga taaatgctga ataataacgc tctatgtttg tcagtagaat
     6121 accacacacc attgaccttt caaacggttt ttttgtcagc attaaaaagg aggtggcttt
     6181 attttttatc gcttcatcga tctcattttc ttccatattg atgttcaatc cgattccaac
     6241 aataacaaaa cggatcatat cagcttctgt ggaaagctca agcaaagttc cacaaatctt
     6301 tctgccatta accagaacat cattaggcca tttgagggcc ggctcaactt tggtcaagga
     6361 ttgaatggta tcatacacgg caagggaaga gaggaatgtg atcgggtata catttgatgg
     6421 agggatttgc ggtctgagca caatggaaat ataaagattc ttgccagcag gtgaaaacca
     6481 tttccgtcca aggcgaccct taccagaatc ctgggtttcg gcaataatgc acgtaccttc
     6541 tgcttctccc ccaatggcaa gcttaaaggc gagggcgttc gttgaatcga gttcatcttt
     6601 gtaaatgatc tttttcccga tgacgtttgt tttacagtat tggtcaatct cccatgcgta
     6661 taacttgtcc ggtgaagcaa tcaatcggta gcccttcccc ttgagctttt cgattctata
     6721 tcccaattgt tcgagatggt ttatatactt ccagattgca gtccgtgaga tgcccataat
     6781 gtttgacata tggtcaccgg acacgtagtc atgtgtgttt cttaaatacg gcaggatttc
     6841 ttttgatcta accattctct atcttgtcat aatgtgaaaa atacattgtg aaagatgctc
     6901 tgccctgaga aagagagcgt acatctgtcg agtacccgaa catttttgtg aggggtgcat
     6961 tagcctgtac aatagtgatt ttatcgctgg tggtcacatt tgtaacctgg ctttttcgtg
     7021 cgttaaggtc actgattatg tcgcccaaaa attcattagg tacagttact gtgagagaca
     7081 taatggggac gagaagtatt ggttttgcca gttggcatgc gtcccgaaag gcctcatagg
     7141 ctgccatttt caaagtaagc cgtgcaaact cagggctatt gaacgttgcg tcacggatag
     7201 aaacctttac atcagtgaca gggaaacctt ttaggatacc catattggag gcttcgagga
     7261 caccctcttc aatagccata agatacgggt gaacagggct ttcttcggat atgctgttca
     7321 ctatttggat tcccccacct ctggtatttg gctgcaccaa aagagaaacc cagcccttac
     7381 aagttacgtt attgataatc ttttcaaagg catgttcaat tgatgcttca tcttcaatgg
     7441 cttccttata aactacttgt ggtttgccta catggaggcc aattccgaat tcgtccttga
     7501 tccttctcac gatgacatcg aggtgaagct cacccatgcc ggacactaca gtctgatatg
     7561 catcttcatc tgttttcaaa acaaaggttg gatcttcatc agaaattctc tggagagcga
     7621 ggaggagctt gtcctggtca acattcctct tgggctccac agcgattgaa ataactggtt
     7681 gatagacttc aatgggttca aggagaagag gttttgcctt ggttagggtt tgaccggttg
     7741 tagtctcttt aaggccaaca atcccaacta tggcgccagt ccttgcctcc tctatcctgt
     7801 ctctgtgatt agcatgcatc cggaagattc ttgatacctt ttccctcttg ccgagattca
     7861 cattatagat gtcatcacca acctttatgg tgccagagta aattctgatg taagtaagct
     7921 ttcttccctc atccaaaacg atcttaaaag caagggcagc aaggtcttct tcgtcactta
     7981 cttctctttg ctccatctgt ccggtttgag gattgattcc ctgaacagga gggatatcta
     8041 agggggatgg aagatatctc acaacactgt ccagaagagg ttggattcct ttattgcgca
     8101 aagcagtgcc acaaagtaca ggaacacatt tcagagctat tgttccctgt cgtaaagtct
     8161 catgaatcat agcatcatcg atttctttgc cttcaaggta tagttccatg aattgatcgt
     8221 cgaggagaga acatttttcc agcattttct ccctctgtga acgagcgagc tgctcatact
     8281 cttccgggat ggaaatcagg ttgaatgtag cccccagatc gtcttcgctc catgccagag
     8341 ccttcattgt gataagatcg attacaccga aaaattcatc ctcccttcca aggggtatct
     8401 ggagcggaat agggtttgcg ccaaacttct ccacaagcat atccatgaca tgaaagaaat
     8461 cagagcctac cctatcaagc ttgttgataa aggcaatacg aggtactctg tatttatctg
     8521 cttgatgcca aacggtttca gactgaggct caactccacc aacgccacaa aaaacagcaa
     8581 ccataccgtc aagcactctg agtgaccgtt caacttcaat ggtgaaatcc acatgacccg
     8641 gtgtgtcgat gagttgaatt tcgtgatctt cccaataaag cgttgtaacg gcagaggtta
     8701 ttgtgatacc cctttcctgt tcctggggaa ggtaatccat aattgtggta ccttcatgga
     8761 cctccccgat tctgtgtgtt ttaccggcat aatacaggat ccgctccgta acggtagttt
     8821 tcccggcatc gatatgtgct gcaatcccta tatttcttat ttttctgagc cttaaaatct
     8881 cttt
//
LOCUS       DAOOMC010000116         8795 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdus sp. isolate AMR_MDS_2685
            NODE_9336_length_8795_cov_5.561556, whole genome shotgun sequence.
ACCESSION   DAOOMC010000116 DAOOMC010000000
VERSION     DAOOMC010000116.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864760
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdus sp. (wastewater metagenome)
  ORGANISM  Syntrophorhabdus sp.
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae; Syntrophorhabdus.
REFERENCE   1  (bases 1 to 8795)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8795)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,729
            Genes (coding)                    :: 2,708
            CDSs (with protein)               :: 2,708
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 7 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8795
                     /organism="Syntrophorhabdus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9336_length_8795_cov_5.561556"
                     /isolate="AMR_MDS_2685"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2699756"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            60..134
                     /locus_tag="PK125_10940"
     tRNA            60..134
                     /locus_tag="PK125_10940"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:96..98,aa:Glu,seq:ttc)
     gene            635..1078
                     /locus_tag="PK125_10945"
     CDS             635..1078
                     /locus_tag="PK125_10945"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HPB38660.1"
                     /translation="MSQIYPARLRKLTMKDLDAVTEIDYSLLGAERKRYWEEKIQLTE
                     SSAVPSLAAEVDGKMVGFILGRASGGEYSIPGNFGWIDTLGVIKEYQKKGIARLLFRQ
                     MLDMFKAVGVDTVYVFVNWKDWDLLQFFDKMGFSRGDMINLELKI"
     gene            1368..1667
                     /locus_tag="PK125_10950"
     CDS             1368..1667
                     /locus_tag="PK125_10950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB38661.1"
                     /translation="MMKVKIKIESNLKIKNIFETPLEIELSEDQATLKDVLQIVSKRF
                     PPYLRFIEKGEMGDDLRQVYLNGQSHFSFSEGLQKKISDGDTVHVEAYMEPLAGG"
     gene            complement(1765..1914)
                     /locus_tag="PK125_10955"
     CDS             complement(1765..1914)
                     /locus_tag="PK125_10955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB38662.1"
                     /translation="MSTLEEDEKSKGNKGAQYKSWLFIISFMIFFFLMGLLVKWFSAN
                     SHSPH"
     gene            complement(2338..2766)
                     /locus_tag="PK125_10960"
     CDS             complement(2338..2766)
                     /locus_tag="PK125_10960"
                     /inference="COORDINATES: protein motif:HMM:NF013607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight phosphatase family protein"
                     /protein_id="HPB38663.1"
                     /translation="MTSTNIKILFVCFANKSRSPMAKALAQKMSSGRLYVDSAGIDSE
                     TSGGANFRAISVMGEYGVDLMGHLAKGINNIPISEFDHVITMDHNVFNSIIQKYPGIV
                     DRTIQWQIEDPFYGTVDSYRVCAEEIFNNLKILLSRLGMF"
     gene            complement(3453..3923)
                     /locus_tag="PK125_10965"
     CDS             complement(3453..3923)
                     /locus_tag="PK125_10965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB38664.1"
                     /translation="MKNIFLLLLSSTVLLFVPHVYGAEWLSIGKDRLGNELFYDPDTI
                     IKLPTGVTKIWIKGIYSMEGKKERIQRRIKSKLPVENYDKLNYVLELQEINCAKREYR
                     VMAYTDYSSDGGILNKFIVDQQTSVGWEPIPPDSMGEIIDRIICPPPSSLQKKR"
     gene            complement(4084..6237)
                     /locus_tag="PK125_10970"
     CDS             complement(4084..6237)
                     /locus_tag="PK125_10970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB38665.1"
                     /translation="MKRLFVALGILFVVCMTIISVSSAQDESPVMVGRIYHIEGPLLR
                     YIDAENDWVAAVPDAPFGAGDTLYTGSQGQAEFVVPNGTWIRIGDNTQIQFIGLDLDV
                     SEVDVASGLARFYNKDDNLVIKSTSPFGYVLVYPGGVFDYYVGENSVEIVPIKGRITF
                     VHTTTNTRYELSPGNPSILADQNQVSSGEGGVDPGWDEWNRSRESFWASRANVTGPSA
                     QYLPPALRHDAYVFEENGRWEKIPYEGSPRWLWRPTRVPVGWAPFTVGRWTEWYGDQT
                     WIPAEPFGYTTHHYGNWIFVRNHWYWAPPVVRATVGLPILNIGLFWYPGRVSWIHYGT
                     HIGWVPLAPRETYYSHYRWGGPHFARVTNINITQINIRPRNYAYARHAIIVPQNNFYT
                     ANNYRAVRITNINTTTIINNYRAAPIVNDKVIKNYSTNRQRFNFANVEVREKPHRTVL
                     ERINTNQKLVQQARREKGSVLQQQAKSIHEGKISREARIQQPKATNFIVPANETNKPR
                     SEIRFQQREFRRAARETKERPVETGKPSSKSIKPVTPPEKSEELPTPLKPRPGRQVPQ
                     PEAIAPEKSVRTTPRPEHMKPSMVPSVEKPQGEATKGITPEREDLAPVKPVTRPKIEA
                     PTKPVQQMRHEAPERRIPKREGVVPAKPEPNVDSEPLDKPSSKRGQIVPAKPDTKIER
                     EAVEKPSPKKERAATTKIRPADKHRGETDEQVKPR"
     gene            complement(6383..>8795)
                     /locus_tag="PK125_10975"
     CDS             complement(6383..>8795)
                     /locus_tag="PK125_10975"
                     /inference="COORDINATES: protein motif:HMM:NF015644.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="UPF0182 family protein"
                     /protein_id="HPB38666.1"
                     /translation="DLRKFQKLFKLFGIGIAIFGGILVALWGSTLWAVILTFFNKMNA
                     GKVDPIFNNEISYYMFSLPLFEILNGFSGLTIFLTILIIAGSYAARGGMLFVGNRISL
                     AKGAKKHLAVLIFLFFLKLAFGFYLHRFDLLYSSHPVLTGASYADIYGRLIALRLLVP
                     LTVAGGIVFCYALLKEKWQGMAYPVIGILSVYILGVAIYPNVLQSFKVAPSEQAMEEP
                     YIKNHITMTKYGYNLENVQVIPFDVSFDLTNKDLEKNNPTIRNIRLWDETPLLRTYSQ
                     LQQIRTYYRFSDIDTDRYIINGEYRQVMLSPRELSYNDLPSKSWINEKFVFTHGNGVV
                     MGPVSRVTREGLPEFYIKDIPPISSSSLQVTNPELYYGELTSDYVVVNTRMPEFNYPT
                     AEGNVYTSYKGSGGILLDSTWRKLLFAAHFKTVKITLSSEITQESKILYNRNVVSRIQ
                     KIAPFLMLDSDPYVVAAKDGKLYWIIDGYTTSDRLPYSKHLKNWVNYMRNSVKAVVDA
                     YNGQVTFYVSDPDDVVIKVYSSIFSGVFKPMNEMEDNLRKHVRYPRDFFRIQTTMYAT
                     YHMTDPKVFYNREDLWEVPVHNDKSMDGNYLIIKLPRETKEEFVILVPYTPAKRDNLA
                     AWFAARCDNEHYGKLIVFTFPRDRLIFGPKQIDARIDQDAYISQQISLWSQRGSSVIR
                     GRLMIIPIENSLLYIQPLFLAAEDKGSLPELRRVIVAYENNVIMEETLEQALNAIFGG
                     RRTAPTSSSAISTSTHRLSIQDIAKEAEQVFEHAKSLQKQGDWAGYGESLKKLEQLLK
                     QMAGK"
BASE COUNT         2411 a         1822 c         1819 g         2743 t
ORIGIN      
        1 gagtatctgc cttttaagca gagagtcgca agttcaagtc ttgcacggcc caaaaatatg
       61 tccccatcgt ctagcctggc ctaggacatc ggcctttcaa gccggcaacg ggggttcgaa
      121 tccccctggg gacgctctca atgatatcaa taaagcacag taggatcaca cgggcaagtc
      181 catgtcacta aaattacaca ctgcgggtga atgaataagt gcatttaccc ctttttttga
      241 aagtatggac cttttatgtg gtttgcctct ctcgtgggca gtaatacagt gaatgacatg
      301 aatttgaggg ggaggctctt taatggcttt attggtgaag gggggcaagg caaaaccccg
      361 taatagattc acaccgtgat ttgaaaatag attccatcaa aatatctcaa cattcagaaa
      421 acaatgtagc atagtcttat taatgtttat gatcatcaaa aatccaagcc ctgaatcctg
      481 accgtctcgt accgcagggc tgtagccctt atcgagtatt aagtgtgttt ttacgattaa
      541 acggcatata cctgacccag caaagcccca tttcttgaca accatcctaa tttgcagtac
      601 attcaaaaat caattagaaa gggggtttga agctatgagc cagatatacc ctgcaaggtt
      661 gagaaagttg accatgaaag atctagatgc ggtcaccgaa atcgactata gccttcttgg
      721 ggcggaaaga aagagatatt gggaggaaaa gattcaactc actgaaagtt ctgctgttcc
      781 ctcccttgct gctgaagtgg atggcaagat ggtcggtttt atcctgggga gggccagtgg
      841 tggggaatat tcaattcctg gtaattttgg atggatagat actctggggg tgattaaaga
      901 ataccaaaaa aaagggatag cccggcttct ctttcggcag atgctggata tgttcaaggc
      961 ggttggtgtg gatactgtct atgtctttgt aaactggaaa gattgggacc ttctacagtt
     1021 tttcgacaag atgggtttta gccgtggaga catgatcaat cttgagctga agatctaaaa
     1081 gaaaagaacg agtaatcagc atgagttgca tagctgtaac ttgtaaggga cggagaccac
     1141 atatatgaac tattgagaag gagatacagg gtgttttgct tacataatta gttgggaatt
     1201 tcctagagtg aatctactat cccatctgcc ttctgtctcc acttggaaac cgaatgagca
     1261 aaattatgga agggatgtcg caaaagctat ttaggagggc ttgatttaac cacacctgac
     1321 aacatatatt cataataaat aactatttaa ataaaaaatt gggttaaatg atgaaagtga
     1381 agataaagat tgaatcaaac cttaaaatta aaaatatctt tgaaaccccc ttagaaatag
     1441 agttaagcga agatcaggca acattaaaag atgttttaca gatagtctcc aagaggtttc
     1501 ctccttatct tcgattcatt gagaaaggag aaatgggtga tgatctccga caggtatacc
     1561 tcaatggaca aagtcacttt tccttttccg aagggttaca gaaaaagatt tctgatggcg
     1621 atacagtgca tgtagaggca tatatggaac cacttgccgg aggctgaaca ttgtttgcga
     1681 tcactgtccc ctattttttc cactttagat aagacagttc aagaaataag aatgacaaaa
     1741 aaatatatca ggagaacata atgtctaatg gggactatga gagttagcgc taaaccattt
     1801 tacgagaagt cccatgagaa aaaagaatat catgaagctg atgatgaaca gccaactctt
     1861 atactgtgct cctttgtttc ctttgctctt ctcatcttct tctaaggtac tcattgtcaa
     1921 aactccttga agtttattgt aaccgtattt tgagtatagc acatattttc atcttggagg
     1981 cagctatctg tatcctttaa tcgaggtggt atacacctct atcctcttta tttttacgag
     2041 ttgcttatct tatagttgtt aaatacctct ttgcgtggct gtgctgggat atggattcac
     2101 acactcgtat cattactcat agttgactga taatacggga tatgcagcct gcaatgtttt
     2161 ccccatagat gacgcaatta caaaaaacag ccagcacatg tattttgcgt aatgcacctg
     2221 tccatgggaa ttttcttttt gttgttggtt ctttagaaaa agttagggga attcgtaggt
     2281 gtaatactat caacactcaa ccctgttatt gacattaaag gttcgttgaa tatttgatca
     2341 gaacattcca agtctggata acaggatttt aaggttatta aaaatttcct cagcgcagac
     2401 tctgtatgaa tcgactgttc cgtaaaacgg atcttcaatc tgccattgta ttgtccgatc
     2461 gactattcca ggatattttt gaatgataga gttgaagaca ttatgatcca tagtaattac
     2521 atggtcaaac tctgagatag ggatattgtt tatgcccttg gcgagatgac ccatgagatc
     2581 taccccatat tctcccatca cactgatcgc cctgaagttg gcgccaccag atgtttcaga
     2641 atcgataccg gcactgtcca catagagtct cccggaggac atcttttgtg caagggcttt
     2701 ggccatggga cttctgcttt tgtttgcaaa acagacaaag aggattttta tatttgttga
     2761 agtcatcgta tcgcctgtta tcttctcctt tcgttctaac catctcaaaa tgtattctat
     2821 tgatgatgcc tctcaggtta tctcttgact ttggcaatgg atatttgagg atattacttc
     2881 attttatttt tcagctcctg ttcgaaataa tcaattaggt tttgcatatg ttccagctct
     2941 tttttgccaa ctgcaacaag ggcttccaat tcctctatcg acattcgctg aagttcttta
     3001 acagtaggaa attgatctaa atttattaat gtgttacaag agtgtttttt catgagtcag
     3061 aatttattga aatcagatat tctccaaatt gaaagctttc tgagcataac aatagtttcc
     3121 caccataatg gatagaacaa tttaatgggg tttgattttt gagcagaatt gacggttcaa
     3181 tcctgtaact ctgcatggag ctgtggtatt cataatcact ttgattaact gttcaagttt
     3241 tctatccatc atgcttgtga aagccatgat ttttttgatt tccttaccgt taattctcct
     3301 ttctacaggt caccaaatgt gtgaccgtga taatctgttg attccttgtt ttaccatgtt
     3361 acccccttta gaaccaaaga tacccacacg gacaagtata cacgcgattt tcattgtgtc
     3421 aaggttcaac gcagaacgta accgcatatt tgctacctct ttttctgcaa tgatgacggg
     3481 ggagggcaga ttattcggtc tattatctct cccattgagt ctggagggat aggttcccat
     3541 cccacagatg tctgttgatc cacaataaat ttgttaagaa ttcctccatc agacgaatag
     3601 tctgtgtatg ccatgacacg gtactctctt ttggcacaat tgatctcctg caattccaga
     3661 acataattta gtttatcata attttcgaca ggtaatttgc ttttaatcct gcgttgaatc
     3721 ctctctttct tcccctccat agaatagatc ccctttatcc atattttcgt aacacctgtc
     3781 gggagtttta ttatagtgtc tggatcatag aacaattcat tacctagcct gtcttttcca
     3841 attgataacc actcagctcc ataaacatga ggaacaaaca ataatactgt ggatgaaagc
     3901 aacaacaaaa aaatattttt caataaaccc tctctctatc caagcagtat atttgtttta
     3961 tagtctcaaa caatattgaa gtcagctgta gcctattagc ttgaactcat tggtttacag
     4021 acgatagctt gacaggtgac taatgattga agagaattac ctcaattttt tatttaaaaa
     4081 aggttatctc ggcttaacct gttcgtcagt ttcacctctg tgtttgtctg ctggacggat
     4141 tttggttgtt gcagcccgct cctttttagg tgagggtttt tcaactgcct ctctttctat
     4201 tttagtgtca ggtttagcag gcacaatttg accccgctta gaagagggct tgtcgagggg
     4261 ttcactgtct acattgggtt ctggtttagc cggcacaacc ccttcccttt tgggaatcct
     4321 tctttctggg gcttcatgtc tcatttgttg aacgggttta gttggcgcct ctatctttgg
     4381 tctcgtaaca ggttttaccg gtgcaaggtc ttcgcgttct ggtgtaatgc cctttgttgc
     4441 ttcaccttgt ggtttctcta cagaaggaac catggaaggt ttcatgtgtt caggcctcgg
     4501 tgtcgttctt acggattttt ccggagcaat tgcttcgggc tgtggaacct gtcgtccagg
     4561 cctgggtttc agcggcgttg gtaactcttc tgatttctct ggtggtgtaa caggtttgat
     4621 tgatttagag gatggtttac cagtttcaac aggtctttct ttagtttcac gggcagccct
     4681 tctaaactca cgttgttgga accttatctc tgatcttggt ttgtttgttt cattcgcggg
     4741 aacaataaaa ttagtggcct ttggttgttg tattcttgcc tcacgactta ttttcccttc
     4801 atgaatactt tttgcttgct gctgtaaaac agaacctttt tccctccttg cctgttgaac
     4861 taatttttgg tttgtattaa ttcgttccag cacagttcta tgaggttttt ccctcacttc
     4921 aacattagca aagttaaatc tttgcctatt agtagagtaa tttttaatta ccttatcgtt
     4981 cacgataggt gcagcacgat agttgttgat gattgtcgta gtattgatat tagttatacg
     5041 aaccgcacga taattattgg ctgtataaaa gttattctga ggtacgatga ttgcgtgtcg
     5101 tgcatacgcg taatttcgcg gacgaatgtt gatctgagtt atatttatgt tggtgactcg
     5161 agcgaaatgt gggccacccc atcggtaatg tgagtaataa gtctctcgcg gggcaagggg
     5221 aacccatcca atgtgcgttc cgtaatggat ccaggagaca cgaccgggat accagaaaag
     5281 gccaatgttt agaattggca atcctacggt tgcccgcaca acaggaggtg cccaatacca
     5341 gtggtttctc acaaaaatcc aattaccata gtggtgagtt gtgtagccga aaggttcggc
     5401 aggtatccat gtttggtctc cataccattc tgtccaccga cctacggtga aaggagccca
     5461 accaacgggt actctcgttg gtcgccagag ccatctggga ctcccttcat aagggatttt
     5521 ttcccatcga ccattttcct caaaaacgta tgcatcatga cgaagggcag gtggaagata
     5581 ttgagctgat gggccagtca catttgctct tgaagcccaa aaactctccc gacttctgtt
     5641 ccattcatcc cagccagggt caactccgcc ctctcctgag ctcacctgat tttgatctgc
     5701 caatattgaa gggtttcccg gggatagttc ataccgtgtg ttcgtcgttg tatgaacgaa
     5761 ggtaattcta cccttgatag gtacaatttc tacagagttt tctccaacgt agtagtcgaa
     5821 gacaccaccg gggtatacaa ggacataacc gaagggactc gtggacttaa tcaccaggtt
     5881 atcgtccttg ttgtaaaatc ttgctaggcc agaggcaaca tcgacttcag aaacatccaa
     5941 atccaaacct atgaactgaa tttgtgtatt atccccaatc ctgatccagg ttccgtttgg
     6001 aacaacaaat tcggcctgtc cttgacttcc tgtgtaaagt gtatctcctg caccgaacgg
     6061 tgcatctggg actgcagcta cccagtcatt ttctgcatcg atataacgga gcagagggcc
     6121 ctcaatgtga tatatacggc caaccataac cggggattca tcttgagccg atgataccga
     6181 gattatagtc atgcaaacta caaataagat accaagagca acgaacaatc ttttcataag
     6241 ctcacccctt gttttgatag gttccattat tatagagact attcattcaa ttttccacca
     6301 aagtatctgc attttttgtc acgggatcct gagataatgc cacagtttct ttttttttct
     6361 tactatccag ctattttcac tgttatttcc ctgccatctg ttttaataat tgttcaagtt
     6421 tcttgagact ttccccgtat ccagcccaat caccctgttt ctgcagagac ttggcgtgct
     6481 caaaaacctg ttctgcctct tttgcaatat cttgaatgga taacctgtga gtcgaagtag
     6541 agatggccga cgaagatgtg ggggcagttc ttcttccacc gaagatagca ttgagtgcct
     6601 gttcaagggt ctcctccatg atgacattat tctcatatgc cacaataacc cttctgagtt
     6661 ctggtagact tcctttgtct tctgctgcca gaaatagcgg ttggatgtaa aggagggagt
     6721 tttctatagg aatgatcata agcctccccc ttatcactga tgaacctctc tgactccata
     6781 gtgaaatctg ctgggatatg tatgcatctt gatctattct tgcgtcgatc tgctttggac
     6841 caaagatgag cctgtctctc gggaaggtaa agacgattag tttcccataa tgctcattgt
     6901 cgcatcttgc tgcgaaccat gctgctaggt tgtccctctt agcaggtgtg taaggtacaa
     6961 gaatgacaaa ctcttcctta gtttctctgg gaagttttat aatcagatag ttaccatcca
     7021 tactcttgtc attgtgaacc ggtacctccc aaaggtcttc tctgttgtaa aagacttttg
     7081 ggtccgtcat gtgataggtg gcgtacatgg tggtctgaat cctgaaaaaa tccctggggt
     7141 atctcacatg ttttctcaga ttgtcttcca tttcattcat cggtttgaag actccagaaa
     7201 atatggagga ataaaccttg attactacat cgtctggatc gctaacgtag aaagtgactt
     7261 gtccgttgta agcatccacc actgccttca cggaatttct catgtaattc acccaatttt
     7321 tcagatgctt cgaatagggc agtctatcgg aggtagtgta gccgtcaatg atccagtaaa
     7381 gctttccgtc ctttgcagct acaacataag gatcggaatc aagcatgaga aaaggagcta
     7441 tcttttggat ccttgaaaca acattccgat tataaagaat cttactctcc tgtgttattt
     7501 ctgatgatag ggttattttt accgttttga agtgggctgc aaagagtaac ttcctccagg
     7561 tggaatcgag aagaatacct ccagaacctt tgtatgatgt gtacacattt ccttccgcgg
     7621 taggataatt aaattctggc attctggtat ttacaacgac gtagtcgctg gtgagttcac
     7681 cataatacaa ttccggattt gttacctgaa gactggaaga cgatatagga ggaatatctt
     7741 tgatatagaa ttctggcagc ccttcccggg ttactctgct aacaggtccc ataacaacac
     7801 cgttaccatg agtaaaaaca aatttttcat tgatccaaga tttgcttgga agatcattat
     7861 aggacagttc cctgggagaa agcatcacct gccggtactc accatttatg atgtaacgat
     7921 cagtatctat atcgctgaat cgataatacg ttctaatctg ttggagctgg ctgtaggtcc
     7981 tgagtagagg cgtttcatcc cagagcctta tgtttcttat tgttgggtta tttttttcca
     8041 gatccttgtt tgtaagatcg aaagatacat caaatggtat tacctgaaca ttttcaagat
     8101 tataaccata ctttgtcata gttatgtgat tcttgatgta tggttcctcc atggcctgct
     8161 cactcggtgc aaccttaaag ctttgaagaa cgtttgggta aattgcaact ccaagaatat
     8221 agacggaaag aataccgatt accggatagg ccatgccctg ccacttttct tttaaaaggg
     8281 cataacaaaa aacaatacca cctgctacgg tcagtggtac caataaacgg agcgctatga
     8341 gacgaccata gatatcagcg tacgaagctc cagtaagaac aggatgggaa gaataaagaa
     8401 gatcaaacct gtgtaaatag aatccgaatg caagtttaag gaagaacaga aagataagaa
     8461 cagcgagatg ttttttagcg ccttttgcaa gtgatattct gtttcccaca aaaagcattc
     8521 ctccacgcgc tgcatagctt cctgcaatga tgagaattgt caaaaaaatg gttaagccgg
     8581 aaaatccgtt caatatctca aagagaggaa ggctgaacat atagtagcta atttcattat
     8641 taaaaattgg gtcaaccttg cccgcgttca ttttgttaaa aaacgtaagt attactgccc
     8701 aaagcgtgct gccccataga gcaaccaaaa ttccaccaaa aatagcaatc ccaattccaa
     8761 aaagcttgaa tagcttttga aatttacgta agtct
//
LOCUS       DAOOMC010000117         8658 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdus sp. isolate AMR_MDS_2685
            NODE_9536_length_8658_cov_6.796815, whole genome shotgun sequence.
ACCESSION   DAOOMC010000117 DAOOMC010000000
VERSION     DAOOMC010000117.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864760
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdus sp. (wastewater metagenome)
  ORGANISM  Syntrophorhabdus sp.
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae; Syntrophorhabdus.
REFERENCE   1  (bases 1 to 8658)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8658)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,729
            Genes (coding)                    :: 2,708
            CDSs (with protein)               :: 2,708
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 7 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8658
                     /organism="Syntrophorhabdus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9536_length_8658_cov_6.796815"
                     /isolate="AMR_MDS_2685"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2699756"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            133..1245
                     /gene="amrS"
                     /locus_tag="PK125_10980"
     CDS             133..1245
                     /gene="amrS"
                     /locus_tag="PK125_10980"
                     /inference="COORDINATES: protein motif:HMM:TIGR04337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system radical SAM enzyme"
                     /protein_id="HPB38667.1"
                     /translation="MINRRQFIKIALITPVALTLDGAQIQGKNDIPMKEAAYYRKIDE
                     KQKKVWCQLCPRGCILSNGELGFCRARKNIDGKLYALGYAQPCALHVDPIEKKPFFNV
                     LPKTLSFSIASAGCNLRCKYCQNWQISQVSPMETTNKYSPPQDIVNAAIKSGSRSIAY
                     TYTEPTNFFEYMLDVAKAAKSKGILNVQHSNGYINPAPLKELCQYLDAVNIDLKGFTN
                     SFYNSLCSAELDPVLETIKTIKASGVWVEITNLVIPGHNDDLNVISKMCAWVKTSVGA
                     DVPVHFSRFYPMYRLTGVPPTPVSTLEKAREAGLAAGLHYVYIGNVPGHPGENTYCPH
                     CKKIVIKRSGYNILENNVKNGKCPHCSEKIGGIWAA"
     gene            1233..2114
                     /gene="amrB"
                     /locus_tag="PK125_10985"
     CDS             1233..2114
                     /gene="amrB"
                     /locus_tag="PK125_10985"
                     /inference="COORDINATES: protein motif:HMM:TIGR04336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein B"
                     /protein_id="HPB38668.1"
                     /translation="MGGVKPVMSCELLVTSCKKAVTRYASRVTIFQLRFVMLFLFILL
                     LSPCVCTGDNWALKVRKPVFAGSFYPADRESLESLIDSYLNETEQKSKKVAGQVFGII
                     APHAGYEYSGKVAAYAYSQIKGKGYQTVIIIGSSHRVPFRGIAIYPEGSWETPLGTVA
                     IDHEMARGIMNSCRVVRSLPAAFEQEHSIEVHVPFLQRTLSGFKIVPVVTGAMNMDDL
                     HLFVDVLSNTIKRNPGNILIVASSDMSHFHSYETANRMDAIALKDIAQLNVDSLRKHL
                     QKEECELCGAQGGFFPF"
     gene            2114..2785
                     /gene="amrA"
                     /locus_tag="PK125_10990"
     CDS             2114..2785
                     /gene="amrA"
                     /locus_tag="PK125_10990"
                     /inference="COORDINATES: protein motif:HMM:TIGR00296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein A"
                     /protein_id="HPB38669.1"
                     /translation="MLARKMNARATILNYANSGDVTKNRSKVVGYCSASFSLPQPDQS
                     LRKKEQEILLGIARMTLEEYVRSGNILKVNVKEPILLEKQGVFVTLTKNGSLRGCIGH
                     TQSAVPLYRAVSDMAIAASTRDPRFPPVSQGELEDIRIQISALSPLKLISDISEIEVG
                     KHGIYMTRGSNTGLLLPQVATEHKWNREEFLKQACIKAGLHDKAWEDKNTRIYTFTAQ
                     VFSEK"
     gene            complement(2802..3341)
                     /locus_tag="PK125_10995"
     CDS             complement(2802..3341)
                     /locus_tag="PK125_10995"
                     /inference="COORDINATES: protein motif:HMM:NF012415.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HPB38670.1"
                     /translation="MKWNAAFIILSSVVLFQAAIAAISRAEEPIGSRLKVEEMSVLLS
                     LHNRVRSDVGIGPLAWSKNLAGYAQSWADHLASTSCKLEHRPISGKWKQEYGENLFIG
                     TLGYYGVADAVRAWEKEKSLYHGAPLTSSNWYPSGHYTQLVWKNTSHIGCAKAECGSN
                     VIVVCNYDPPGNVLGQKPY"
     gene            complement(3442..4806)
                     /locus_tag="PK125_11000"
     CDS             complement(3442..4806)
                     /locus_tag="PK125_11000"
                     /inference="COORDINATES: protein motif:HMM:NF012312.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPB38671.1"
                     /translation="MDQKPRENAFQFLDKLQFNRFHVQLMIFTTLSSVVMGIISQTVA
                     YILPLVLKEWSLTPVEGGSIASYTFVGLMLGAAGFGILSDRIGRKKTLILAVAISGIF
                     NGLAFFAPDYTVFCVLRFLSGLGLGGIPPLSVTLLSELVPTRMRAKAVTTATCGFPFG
                     WALSGVVAMFVVPHFGWRVLLAVSGLIAMALIPFLIVYLPESIRFLSGKGRNEEAGEQ
                     MRRIEKTARLTASQWTFDSSTQGARQVKGALSQLFSTKLATMTILVWLSYFFTLLTVH
                     GLSVWIPQLFVKAGYSLVKSYSFSIVQSMWAMVGGFFLGFLLDHFGRKPGLLLSYMFG
                     GFSILIFSIADSPAFLYLAGIATGLFVLPLPSVLHVVAGEIYPTSIRATGVGWAAVAS
                     RVGSILGPIFGGAIQMAGLGFHTFFFIFALPCFICMIFVVFYRVNVKNDALEEVSSKL
                     MSRS"
     gene            complement(4940..5089)
                     /locus_tag="PK125_11005"
     CDS             complement(4940..5089)
                     /locus_tag="PK125_11005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB38672.1"
                     /translation="MSRATKANTGATAVLGTSAMQAYLPFTLHKFRRKSRGTFELFFS
                     ECVEE"
     gene            5182..5673
                     /locus_tag="PK125_11010"
     CDS             5182..5673
                     /locus_tag="PK125_11010"
                     /inference="COORDINATES: protein motif:HMM:NF012417.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HPB38673.1"
                     /translation="MLKKVLIVLLLFFPVGSLVAGETGAREKGADYGRQPWVVDIEDL
                     TEKNENFRTAWWTGKNLQMTVMSIKPRGEIGLEKHDKGDQFIRVEKGKARVVMGKSKD
                     KMTFDKRISDDWAIFIPEGFWHNIINEGDKPLKVYVIYGPPEHPAGTLHKTFEESEAA
                     HSH"
     gene            complement(5828..6049)
                     /locus_tag="PK125_11015"
     CDS             complement(5828..6049)
                     /locus_tag="PK125_11015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB38674.1"
                     /translation="MLNLTIPFMISIICFMDVNEKVKKAVKQPDNMVYYRPRDELYST
                     LHTCVVLRELPELSPEELQQIAEWEQKSK"
     gene            6152..6844
                     /locus_tag="PK125_11020"
     CDS             6152..6844
                     /locus_tag="PK125_11020"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPB38675.1"
                     /translation="MQPIVRVVDIWKQYGDEGQNGIALRGTNLEIKPGEVVALFGKSG
                     SGKTTLLNLIAGLDRPTKGRIEVEGRDLESIGETGRTDLRRRRLGFIFQFFNLLPTLT
                     AYENVYLSLELLGRRDQKSAKDALAAVGLEGKGARYPHELSGGEQQRVAIARAIVKEP
                     TLILADEPTGNLDTTTGSQILELLQHRCQKHKMSLLMVSHTPLTWKFADRILHMVDGV
                     ITEPASCTEITR"
     gene            6841..>8658
                     /locus_tag="PK125_11025"
     CDS             6841..>8658
                     /locus_tag="PK125_11025"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HPB38676.1"
                     /translation="MRVVTKSFLRYLIKRPGLSVLQLLGIACGVAAVIGMTVSARSAL
                     TSFAMAVDFLRGKSTHVMERPAGPLDEITLEKLMLDPAVRYFSPVVDRKIKLKTGESV
                     RVFGIDPFLDRSIRPELSNAPFSETDVKNRQTYIDFLLKETTIFLEKNLATDLGLKPG
                     EMLSTSRGDFQILGLFANPSGEPIILMDIAHAQRLFDLSGKIDRVDLILSDEAGFLPR
                     WKEGYRIQSHSQKKETLLAMLAAFRLNLEALSLLALFVGVFLIYNTTTFAVVSRRRDA
                     GILRSLGAQNSEIVLSFLAEILIFGILGGALGAIIGHLLAHFLVNLVGSTISNLYFFV
                     RPVEPPWSIMTILAGVIIGGGASLLGGFLPLLELIRIDPVQTLSGRTVSRKSRIKTQR
                     VAFAGLAIIVVSIILLLLSFINVYVGFAGVFAFILGFSLLTGIILIALGPAMKWLFYH
                     LGGLPGKVAAGNIRQNLSRTNIAVSAFMIALALSIGLGSMIGSFRKSLVWWMGTQLRG
                     DLYISTIAEIQVPEEFYEELKAMPGLGGIDPYRNVQVMYNGFQISISSIDASVLQRYA
                     RFGWLKGGNENWEAVKNGGVVISESFARRFKTKEGDRVTL"
BASE COUNT         2411 a         2001 c         2029 g         2217 t
ORIGIN      
        1 tcttggtggt ttcctcctgg ttcctacact tggcatagca aaaacctgcc tcaccctggg
       61 catcctcaaa gtatgcagcc tcttactcct gttcactttc ccgcgaaagt agtataattt
      121 tatgcagaca ccatgataaa cagaaggcag ttcattaaaa ttgcgcttat tacacctgtg
      181 gcattgaccc ttgatggagc tcaaattcag gggaagaatg acattcccat gaaggaggca
      241 gcttattacc ggaaaatcga tgagaaacaa aagaaggtct ggtgccagct ctgtccccgg
      301 ggatgcatct tatcaaacgg agaattgggg ttctgcaggg ccagaaaaaa cattgacggg
      361 aaactttatg ctctcggtta tgcccaaccc tgtgcattac atgttgatcc tattgaaaaa
      421 aaaccgtttt tcaacgttct gccaaaaacc ctgagctttt ccatcgccag cgcaggatgt
      481 aatctccggt gcaaatactg tcaaaactgg cagatttccc aggtatcccc aatggagaca
      541 acaaacaagt atagcccacc acaggacata gtcaatgcag ctataaagag tggaagcaga
      601 agtatcgcat atacatacac agagccaaca aactttttcg agtatatgct tgacgtggca
      661 aaggctgcta aaagcaaagg aatactaaat gttcagcact caaatggcta tatcaaccct
      721 gctcccctga aagaactctg ccagtatctg gatgctgtta atatcgatct taaaggcttc
      781 acaaactcct tttacaacag cctgtgcagt gcagagctgg acccggtgct tgaaacgatt
      841 aaaaccatca aagcttctgg tgtatgggtc gaaatcacca accttgtcat cccgggacat
      901 aacgacgatc tgaatgtgat cagcaaaatg tgcgcatggg tgaaaacaag cgttggcgct
      961 gatgttcccg tccacttctc ccgtttctat cccatgtata gattgactgg tgttcccccc
     1021 acgccggtta gcacgctgga gaaagctcgg gaagcaggac tggctgcagg acttcattat
     1081 gtttacatcg gtaatgtccc cggtcatccc ggagaaaata catactgtcc ccactgtaaa
     1141 aagatcgtca taaaacgatc cggatacaat atactggaaa acaatgtgaa gaatgggaaa
     1201 tgcccccact gtagcgaaaa gatcggaggg atatgggcgg cgtgaagcca gttatgagtt
     1261 gcgagttatt agttacgagt tgtaaaaagg ctgttacgcg ttacgcatca cgcgtcacga
     1321 tctttcaatt aagatttgtc atgctctttc tgtttatcct cctcctttcc ccctgtgttt
     1381 gtacaggaga taattgggct ttaaaggtgc ggaaacctgt ttttgctggg tcattctatc
     1441 cggcagacag agaaagcctg gaaagtttga tcgacagtta cctcaatgag acagaacaga
     1501 agagcaaaaa ggtagctggc caggtttttg gtatcattgc accccatgca ggctatgaat
     1561 attcaggaaa ggttgctgct tacgcatatt ctcagatcaa aggcaaaggt tatcaaacag
     1621 tcattattat cggctcaagc caccgtgtcc ccttccgggg aatcgccatc tacccggaag
     1681 gatcgtggga gacaccactg gggacagttg caatcgacca cgaaatggcc cggggtatta
     1741 tgaacagctg tagggttgtc aggtcccttc ccgcagcctt tgaacaggaa cactccattg
     1801 aagtacacgt gccgtttctt caaagaaccc tgtcaggatt caagatagta cctgttgtta
     1861 caggagcaat gaacatggat gacttacatc tgtttgttga tgttctttcg aacaccataa
     1921 aacgcaaccc ggggaacata ttgattgtgg catcatcaga catgtctcat ttccactcct
     1981 atgagactgc taacagaatg gatgctattg ccctcaagga tatcgctcaa ttgaatgttg
     2041 acagcctccg gaagcatctt cagaaagaag aatgtgaact ctgtggagca cagggggggt
     2101 tctttccctt ctgatgctgg ccaggaagat gaatgccagg gccaccattc tcaactatgc
     2161 taattcaggg gatgtgacaa aaaacaggtc aaaggtcgtg ggttactgct cggcatcctt
     2221 ttcactccct caacctgatc agagtctcag aaaaaaagag caggaaatac ttctcggaat
     2281 tgcacggatg actcttgagg aatatgtacg gagtgggaat attttaaagg taaatgttaa
     2341 agagccaata ctcctcgaga aacagggtgt gtttgtaact ttgacgaaaa atggatcact
     2401 gaggggttgc atcggccaca cccaaagcgc agtacccctt tacagagctg tttcagacat
     2461 ggctattgcg gcatcaacaa gagaccctcg cttcccgccc gtctctcaag gagagctgga
     2521 ggatattcgt attcaaatat ctgccttgag ccccttaaag ctgatttccg atatcagcga
     2581 aatcgaggtt ggcaaacatg gcatatatat gaccagaggc agtaataccg gactgctcct
     2641 gccccaggta gcaacggagc acaaatggaa cagagaggaa tttctgaaac aggcatgcat
     2701 taaagctggt ctgcacgaca aagcctggga agacaaaaat actcggattt acacattcac
     2761 tgctcaggtt ttttcagaaa aataaccgat caacgccagc ctcaatacgg cttctgcccg
     2821 agcacattac ccggtgggtc gtaattgcag acaactatga cgttactgcc acactcggcc
     2881 tttgcacaac cgatatgact cgtatttttc cataccagtt gagtatagtg gccagaaggg
     2941 taccagtttg atgaggtgag gggagcaccg tgatataatg acttttcttt ctcccatgcc
     3001 ctgacagcgt ctgctacgcc gtaatatccc agggttccta taaacaggtt ctcgccgtac
     3061 tcttgtttcc atttaccaga gattgggcgg tgctccagct tgcaactggt tgaagcgagg
     3121 tgatctgccc atgattgcgc atatcctgcc agattctttg accatgcaag aggtccaatc
     3181 cccacgtctg agcgtaccct gttgtgaaga ctgaggagaa cactcatctc ctctaccttt
     3241 aggcgagacc caatcggctc ttcagccctg gatatggcag caatcgctgc ctgaaagagt
     3301 accacagagg aaagtataat aaaggctgca ttccatttca taatcttttt atgccagctc
     3361 tgcttttcgc tgatggatgt gatgacacaa cccggcccaa ccccattctg cctaaaccaa
     3421 tattatgccc tgctctctgt cctaactcct tgacatcaac ttggagctta cttcttcgag
     3481 agcatcattt ttcacgttga ctctgtagaa tacaacgaaa atcatgcaaa tgaagcaggg
     3541 cagtgcaaaa atgaaaaaga aagtgtggaa acccagtcct gccatctgaa ttgctcctcc
     3601 aaatatcggc ccgagaatag agcccaccct cgatgccact gctgcccacc cgacaccggt
     3661 tgcacggatg ctggtcgggt agatctcccc ggccacaaca tggagaacac ttggaagggg
     3721 caatacaaat aaacctgttg caataccagc aaggtaaaga aatgcaggag aatctgcaat
     3781 actaaatatg agaatggaaa aaccgccaaa catataggag agaagaagac cgggtttacg
     3841 gccaaagtgg tcaagcagaa agccaagaaa aaaacctccc accatcgccc acattgactg
     3901 tacaatgctg aagctataac tcttaaccag ggaatagcct gcctttacaa atagctgagg
     3961 aatccatact gataagccat ggacagtaag caaagtaaaa aagtaactga gccacacaag
     4021 aatcgtcatt gttgctaact ttgtgctgaa aagctggctt aatgcccctt ttacctgtcg
     4081 agcaccctgt gttgaactat caaaagtcca ctgagaagcg gtgagccggg ctgtcttttc
     4141 aatcctgcgc atctgttctc ctgcctcttc attacggccc tttcctgaga gaaaacgtat
     4201 tgactcgggt aaatagacaa ttaagaacgg aataagggcc attgctatca ggcctgacac
     4261 tgcgagtaaa actctccatc cgaagtgagg gacaacaaac atagccacaa caccagatag
     4321 agcccatcca aaaggaaacc cacatgttgc tgttgtaact gcctttgctc tcatccgtgt
     4381 gggtacaagc tcagacagga gggtaacact aagagggggg atacctccca acccaagccc
     4441 ggaaaggaaa cggagaacac aaaaaacagt ataatcaggt gcaaaaaagg caaggccgtt
     4501 gaaaataccc gagattgcta ctgcaaggat caatgttttt tttctgccta tgcggtctga
     4561 gagtatgccg aaacctgcag cgccaagcat gaggccaaca aatgtgtagg atgcaataga
     4621 tccgccctca acaggggtaa gactccactc cttgaggacc agtggtaata tatatgccac
     4681 tgtctgagag atgattccca taaccaccga ggaaagagtc gtgaatatca ttaactggac
     4741 gtgaaaacga ttgaactgaa gtttatcgag aaactgaaat gcgttctctc ttggtttttg
     4801 atccactata tgctacacct cttattcgag attgtactgt gcgaaaatat ctatgaagaa
     4861 tatcggaaat taacacaata tgcaataaat attaagactg acaattgtcg ggcatcgatt
     4921 tttgtcaacc gaaccatctt tattcttcaa cacactcgga aaagaaaagt tcaaaggttc
     4981 cccggctttt tcgtcgaaac ttgtggagag taaatggaag atatgcttgc atggcagagg
     5041 tgcccaaaac tgccgtggca cctgtgttcg ctttcgtagc tcttgacata tatgcatccc
     5101 ttactgttta tatagaatgt actgggccga atctatgccg gtacacagaa aaaaaattcc
     5161 atctgaaagg aggcttgggg tatgttgaaa aaggtattga ttgttctgtt actgtttttt
     5221 ccagtaggtt cattagtagc tggagaaacg ggtgcaaggg aaaaaggagc ggattatggc
     5281 aggcaaccat gggttgtcga cattgaggat ttaacagaaa aaaatgaaaa ctttcgtacg
     5341 gcttggtgga cagggaaaaa ccttcagatg accgttatgt caatcaagcc ccggggagag
     5401 ataggccttg aaaaacacga taaaggagac cagtttatcc gtgtagaaaa gggcaaggct
     5461 cgtgttgtaa tgggcaaatc aaaggacaaa atgacttttg acaaaaggat ttcagatgat
     5521 tgggcaattt tcatccctga gggtttttgg cataatatta tcaatgaggg ggataaacct
     5581 ctgaaggttt atgtgattta cggaccccca gagcatcctg caggaacgtt gcacaaaacc
     5641 tttgaggagt cagaagctgc ccacagccac taaagcgcag atctaaaaga atatattgaa
     5701 gtatctctca ggctgcggga taaaagaaag gttgttcagt ccggagtgca cagccatctg
     5761 tttctttttt ggttttggtt tttacgcaag tagttcttac tgagacgctc tttcaaagcc
     5821 acactgttta tttggatttt tgctcccatt ctgctatctg ttggagttct tcagggctga
     5881 gttcaggtag ttcacggagc actacacaag tgtggagtgt tgagtacagc tcatctcgtg
     5941 gtctgtagta aaccatatta tcaggttgtt ttacagcctt ctttactttt tcattaacgt
     6001 ccataaaaca gatgatacta atcatgaaag ggattgtcaa attgagcatg atgtaatgca
     6061 attaggagag tttatatgta ttataataat gtaaaaaaat cattgaaatt cgtgttataa
     6121 ctgactatac cgcattgtgg aggtttagaa aatgcagccg attgtaaggg ttgtggatat
     6181 ttggaaacaa tacggcgatg aaggtcagaa tggtatcgca cttcggggaa caaaccttga
     6241 aataaagcct ggagaggttg ttgcgctctt tggcaaaagc ggttccggca aaacaacgct
     6301 cctcaacctg attgcaggtc ttgaccgacc aacaaaaggt cgtatcgaag tagagggacg
     6361 cgatcttgaa agtatcgggg aaacaggcag gacagatttg cggcgccgac ggctgggatt
     6421 cattttccag tttttcaatc ttcttcctac acttaccgcc tatgagaatg tttatctctc
     6481 tcttgagctg ctgggcagac gtgatcagaa atctgccaaa gatgcacttg ctgctgtagg
     6541 cctcgaagga aaaggagcac ggtatcccca cgaactgtca ggaggtgagc aacagcgcgt
     6601 tgcaattgcc cgcgccatag tcaaggagcc aacgctcatt ctcgctgacg agcccactgg
     6661 aaatcttgat acaaccactg gaagccagat tcttgaactt cttcagcacc ggtgccagaa
     6721 acacaaaatg tcccttctta tggtttccca tacaccctta acatggaaat ttgctgatcg
     6781 gattctccac atggtggacg gagtaatcac tgaacctgcc tcctgcacgg agattacacg
     6841 ttgagggtag ttaccaaatc ctttctccgc tacttgatca aacgcccggg tctttcagtt
     6901 ttgcagctcc ttggaatagc ctgcggggtt gccgcagtga taggtatgac tgtatcagcc
     6961 cgttctgccc tcaccagctt tgccatggca gtcgatttcc tgcggggaaa atcaacccat
     7021 gttatggaga ggcctgcagg ccctctggat gaaataactc tcgaaaagct catgcttgat
     7081 ccggctgtaa ggtatttctc accggtggtc gatagaaaaa taaagctcaa aacaggagag
     7141 tcggtaagag ttttcgggat tgatcccttt cttgaccgtt ctattcgtcc tgagctgagc
     7201 aatgccccct tcagcgaaac agacgtcaaa aaccggcaga cgtacatcga ttttctgctg
     7261 aaagagacaa caatttttct cgaaaaaaac cttgcaaccg atctgggctt gaaacctgga
     7321 gagatgcttt ccaccagcag gggcgacttc caaatccttg gactttttgc aaatccttcg
     7381 ggtgagccca ttattcttat ggatatagcc catgcacagc gcctgttcga tctcagcgga
     7441 aaaattgatc gggtagatct catcctctct gacgaagcag ggttccttcc acggtggaag
     7501 gaaggttatc gcatccagtc ccacagtcaa aaaaaggaga cccttctggc aatgcttgct
     7561 gccttccggc tcaaccttga agcgctctcc ctcctggcgc tctttgtagg cgtttttctt
     7621 atctacaaca caacaacctt tgccgtagtg agccgccgga gagatgcagg catcttaagg
     7681 agtctggggg ctcaaaatag tgagattgta ctctcctttc tcgctgaaat ccttatcttt
     7741 ggtattctgg gcggggcgct tggtgccatc attggccatc tcctggctca tttcctcgtt
     7801 aaccttgtgg gaagcaccat cagcaacctc tattttttcg tccgtccagt tgaacctcca
     7861 tggtcgatca tgaccatcct tgcaggtgtc ataataggcg gcggagcaag cctccttggt
     7921 ggatttcttc cccttctcga gctgattcga attgaccctg ttcagacatt aagcgggaga
     7981 actgtgagcc gtaagagcag aataaaaaca cagagggttg cctttgccgg ccttgctatc
     8041 attgtggtga gcatcattct tcttcttcta tcatttataa acgtatatgt gggattcgca
     8101 ggtgtttttg cgtttattct cggtttcagc ttgttaaccg gtattatact gattgccctt
     8161 ggcccggcca tgaaatggct tttctaccat ctcggcggac ttcccgggaa ggtggcggca
     8221 ggcaatattc gtcaaaacct gagcaggaca aacattgctg tttctgcctt tatgattgcc
     8281 cttgctttat ccattgggct tggttccatg attgggagtt tccggaagtc actcgtctgg
     8341 tggatgggaa ctcagctgag gggagatcta tacatttcta ccatcgcaga gatacaggtt
     8401 ccagaggagt tttacgaaga actcaaggcc atgcctggcc tgggaggaat agatccgtac
     8461 cgcaatgtcc aggttatgta caacggtttt caaatctcaa tttcctccat tgatgcctct
     8521 gtgttacagc gttatgcccg tttcggttgg ctgaagggcg gcaacgagaa ctgggaggct
     8581 gttaaaaatg gcggggttgt tatttctgag agctttgcca ggaggttcaa aacaaaggag
     8641 ggagacaggg tcaccctg
//
LOCUS       DAOOMC010000118         8551 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdus sp. isolate AMR_MDS_2685
            NODE_9694_length_8551_cov_7.149835, whole genome shotgun sequence.
ACCESSION   DAOOMC010000118 DAOOMC010000000
VERSION     DAOOMC010000118.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864760
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdus sp. (wastewater metagenome)
  ORGANISM  Syntrophorhabdus sp.
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae; Syntrophorhabdus.
REFERENCE   1  (bases 1 to 8551)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8551)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,729
            Genes (coding)                    :: 2,708
            CDSs (with protein)               :: 2,708
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 7 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8551
                     /organism="Syntrophorhabdus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9694_length_8551_cov_7.149835"
                     /isolate="AMR_MDS_2685"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2699756"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..600
                     /locus_tag="PK125_11030"
     CDS             <1..600
                     /locus_tag="PK125_11030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB38677.1"
                     /translation="FALLGALFGGGEDLSFVEFDYGRSSLTDTAIKKLETLNKALQDR
                     PAVNVEIVGYVDPEKDREGLKNLLLSRRVKAQKAKDLAKKSEAPARVDDVKIGPEEYE
                     KYLRLAYEAEKFAKPRNIIGLTKKLPVPEMEKLMLTNIAVKDEDLRNLSLERATSVKD
                     FILRSDKVAPNRLFIVQAQNFTPEKKENLKASRVDFRIK"
     gene            complement(738..1460)
                     /locus_tag="PK125_11035"
     CDS             complement(738..1460)
                     /locus_tag="PK125_11035"
                     /inference="COORDINATES: protein motif:HMM:NF015325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HPB38678.1"
                     /translation="MNIIALNGSARGLRGVTWKLLNRLLKGLEEGGAQTHAIQLKDHV
                     ISPCSACLTCMHKTPGLCAINDDMDTIYPYLKDSDILIMATPVYVDNMSAQMKAVMDR
                     CICCLEPFLRIDTADRVRHPFIWNMPGKFFLVSTSGFPETESFAPLIQTYRAQAANFG
                     SYPIGEICIPGSIALQMAPHLLEPRLDMIQHAGKMLSKQGGVDPSLLEEINKPIVTRE
                     KYLEIAAQYEAWARKSRGETGR"
     gene            1680..2021
                     /locus_tag="PK125_11040"
     CDS             1680..2021
                     /locus_tag="PK125_11040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB38679.1"
                     /translation="METEIDLIPSELGAIETHKYFLSEKEGREISFDEAMADFLHNYK
                     ADFLSKKLFEDNQKQHQEIQKYKWIESEKAGHDIGKAKAAMEWIEKYGSIWREERVTR
                     EERIHQSESRN"
     gene            1906..2307
                     /locus_tag="PK125_11045"
     CDS             1906..2307
                     /locus_tag="PK125_11045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HPB38680.1"
                     /translation="MTLEKQKQPWNGLKNTVAFGVKRESLEKNGFISQRVEIKHRCGA
                     YIDTTELATIAHTFGCDIYIHKNRMEQYNFTLFSKKKYLNVRSILTPKFLEAFYGETI
                     ELIATGGGAKDALEASVRLLNESPPCFPAKD"
     gene            2338..4383
                     /locus_tag="PK125_11050"
     CDS             2338..4383
                     /locus_tag="PK125_11050"
                     /inference="COORDINATES: protein motif:HMM:NF024938.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HPB38681.1"
                     /translation="MKKFFYPESIVVFGVSPTDNNLARIIVDNLDRFQFSGKVFPVGT
                     KKGELGRRRVFTSVHEIDETLDLAVILVPGRFVPETLMACAKKGIRHVIIESGGFTEL
                     DVSKKVLEDQIQAISEQHTMQVIGPNCFGVINLEAGVVLPFFVLNPGYMKKGTVSLVS
                     QSGGIFYDTSMLASCEGVGLNKLISIGNKLLTDEIVCLEYLINDKKTAIIGLYLENFI
                     NGRRLMDLAAQTTKPIVCLKANRSPAAEEVARFHTTALAGDDRIADAALGQAGIHRVQ
                     NFREMMDAFKVFSLPIIKGNRLALITRSGGHGVLSGDATHRYGFVPASLSESFFDLVK
                     SKKINVIRTTNPVDVGDVYSLNVYGEIFEKALQEENVDGVIFVATFSSETDSVAVEKA
                     LKDAARLTPIYGKPIVFCMVTNKDRWFHLKEAADFPVFTDVDDALKALALSREHWNNL
                     GKPTRTGKTMSFLRHKKVLKNMNAPGLLDSTHSFSLVQEYGIAVAEYSLATTHSEAME
                     NARKIGYPVALKTTSPSVLHKTEHNGVFLNIKNDRELSKALGKIKTDTYFIQKMAPAG
                     TEVIVGGKRDREFGPVILFGLGGIFVEVFQDTLIRVAPVDLVTAAQMIDSINGSRLLK
                     GFRGSKPSDIEVLAHVLVNISTLLMDHPEITDLDINPLIVLEKGRGCIAVDVKIQIS"
     gene            complement(4756..5613)
                     /locus_tag="PK125_11055"
     CDS             complement(4756..5613)
                     /locus_tag="PK125_11055"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014414.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HPB38682.1"
                     /translation="MKRRVVHIASILAFCFVLFSCDSIPGKKTIPDVHFATTPHKVVK
                     EMLKLAEVSRDDTVYDLGSGDGRIVIAAARDFGARGVGVELDKELIGESVKNAKEAGV
                     TEKVKFLEEDFFTVDLHDATVVTLYLLPDLNARLLPKFLKELKPASKIVSHMWEMGKW
                     KPDMTLKAYGTTVHLWVVPAWVDGNWRITLNKQTESRGYKLAIKQDFQKIRATLSDEK
                     RRYNVEDTNIEGNDIAMTVSDAVRGRVFIINMEAVVKGDTMEGTAFVREGDTPFAEKY
                     SMIGERIAQ"
     gene            complement(5618..6925)
                     /locus_tag="PK125_11060"
     CDS             complement(5618..6925)
                     /locus_tag="PK125_11060"
                     /inference="COORDINATES: protein motif:HMM:NF024910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HPB38683.1"
                     /translation="MKKTLSVLDVVALIVGIVVGVGIFKTPSLVAANAGSVGTFLLTW
                     VLGGAISFVGALCYAELATAYPHPGGEYHYLTRAYGPRVSFLFAWARMTVIQTGSIAM
                     LSFVLGDYMSRIYSLGPYSASIYGAAGIIILTVINIIGIREGKTTQNLLTVTKIIGLL
                     IVVIGGVFFSSPFSTAQEQPYLTGHVFGLAMIFVLLTYGGWNEAAYISAELKGSGRGM
                     VHSLLWGILIITSIYVLINWVYVHVLGIVSVGKSDAIVFDVMVRIVGHYGAVLSTVLI
                     AISALGAMNATIITGARTNYALGTEFSIFRFLGAWHESSGTPANALIVQGAISFFLVV
                     LGSITMKGFVTMVEYTASIFWLFFFLTTLSIIILRLKEPEAKRPFVVPGYPVTPLLFC
                     AICIYMLVSSIQYTGIGALTGIAVFLTGTFFMKLSKHHVTKRR"
     gene            7367..8539
                     /locus_tag="PK125_11065"
     CDS             7367..8539
                     /locus_tag="PK125_11065"
                     /inference="COORDINATES: protein motif:HMM:NF018485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPB38684.1"
                     /translation="MKSNRIVFFVLGWIGMLLYFTQRWIFGPLIPPLMEEFHTDRTAL
                     GIIGAASLWGYMLTPIAAGVLSDRFGRKCAIIIGIFGFSLLTVVCGFSNGPRFLFFGR
                     FLTGMVEAFYFIPMLAFTLELFPERPGFFLTTLSSGSSLGWFVGPALSGWLLDLTGSW
                     RVPFFVAGLAGILVAIFLLTTWPDRDKASTTIRYFDRAILKRANLVMLVFLSLTGMFQ
                     ISAEFGFTMWYPAFLKTELGFAATAAGLVAGLYGIGQCIGRPILGWVSDRLGYRRVGI
                     FCAFIMGITLMIILATNNPVLRAYCTFQAGFIGAAMMGAVWTFTGLVFPTFKGLALGV
                     ITTFGYATASLAPISIGYIGDHYSISTGLWVIAVPAVFLGALSLLSTFLIRPTTKR"
BASE COUNT         2375 a         1975 c         1911 g         2290 t
ORIGIN      
        1 tttgcacttc tgggggcgct ctttggcgga ggtgaggact tgagtttcgt agaatttgat
       61 tatggcaggt ccagtctaac ggacactgcc ataaaaaaac ttgaaaccct caacaaagca
      121 ctccaggata ggccggcagt aaatgttgag atagtcggtt atgttgatcc tgaaaaagat
      181 cgtgagggtt tgaaaaatct tttactttcc aggcgagtta aagcccaaaa agcaaaggat
      241 cttgccaaaa agagcgaagc ccctgctcgc gtagacgacg tgaaaatagg ccctgaggag
      301 tatgagaaat accttcgatt ggcttacgag gccgagaaat tcgccaagcc aagaaatatt
      361 atcggtctga caaaaaaact ccctgttccg gagatggaaa aactgatgct gacaaatatt
      421 gctgtgaagg atgaagatct acgtaacctg tccctggaac gggctacatc ggtaaaggat
      481 tttattctta gatcagataa ggtggcaccg aacagacttt ttattgtaca agctcagaac
      541 ttcacccctg aaaagaaaga aaatctaaag gcaagcaggg ttgattttag gataaaatag
      601 caggaagttt tagatccaac cctgatctac catttgaatg tgcatcttcg tcagttattc
      661 tcccaaattc attcacaatg aaacgaactg ttcggggata aggaaggttt gactgatcac
      721 cgattacaga tcaggcatta ccggcccgtt tcccccctgc tctttctcgc ccaggcctca
      781 tactgagcag cgatctccag atatttctct cgtgttacga ttggcttgtt gatctcttcc
      841 aaaaggcttg ggtcaacccc tccctgtttt gatagcatct tccctgcatg ttggatcata
      901 tcaagacgag gttcaagtag atgtggcgcc atctgcaagg caattgatcc aggtatgcag
      961 atctcaccga tgggatagct gccaaagtta gcagcctggg cccggtaggt ttgaatgaga
     1021 ggtgcaaagc tttctgtctc tggaaatcct gatgtggaga caagaaaaaa tttaccaggc
     1081 atgttccaga taaagggatg gcgtacacga tctgcagtat caattctcag aaaaggttcc
     1141 aggcaacaga tacatcgatc cataaccgct ttcatctggg cactcatgtt gtcaacataa
     1201 acgggcgttg ccatgataag gatatctgag tccttcaaat acggataaat tgtatccata
     1261 tcgtcgttga tggcgcaaag tccaggtgtc ttgtgcatac atgttaaaca tgcactacaa
     1321 ggcgagataa catgatcctt aagttgaatg gcatgagtct gggcaccgcc ctcttcaagg
     1381 cccttcagaa gcctattcag cagcttccat gtaacccccc tcaatcccct ggcgcttcca
     1441 tttaacgcga ttatattcat gattccccac ctgtcatact tttttcagtt acctctggtg
     1501 caacctgctc atatcctgtc tgtgaagtat ttgcattaat agacgataat atatcgtatg
     1561 atgtgtatag tatttgttgc aacaggaatt ttacataaag atatggctgg caacacaaaa
     1621 attttgtatc ctatacgcga ttttacggat aactgcaaat agttttttga ggaaactgca
     1681 tggaaacaga aatagacctc atcccgtctg agcttggcgc aatagaaacc cacaaatatt
     1741 ttctctcgga aaaagaagga agagaaatca gctttgacga agctatggct gattttcttc
     1801 acaattataa ggctgatttt cttagtaaga aattattcga agataaccag aagcagcatc
     1861 aggaaataca gaaatacaaa tggattgagt ctgagaaggc aggtcatgac attggaaaag
     1921 caaaagcagc catggaatgg attgaaaaat acggtagcat ttggcgtgaa gagagagtca
     1981 ctcgagaaga acggattcat cagtcagaga gtagaaatta agcatagatg tggcgcttac
     2041 atcgatacaa cagagcttgc aacgatagcc catacttttg gctgtgacat atatatccac
     2101 aaaaacagga tggaacaata caacttcaca ctattcagca aaaaaaagta tttaaatgtt
     2161 aggtcgatac tcaccccaaa attccttgaa gctttttacg gtgaaactat tgagttaatt
     2221 gcaacagggg gcggggcaaa agatgccctc gaagcctctg tgcgcctcct caacgaatcg
     2281 cctccttgtt ttcctgctaa agactgagta tactaaatcg gtttaaggct aatctctatg
     2341 aaaaaattct tctatccgga aagcattgta gtttttggcg tctccccaac tgacaacaat
     2401 cttgcacgta tcatcgtgga taatctggat cgcttccaat tttcaggtaa ggtatttcct
     2461 gtgggaacaa aaaaaggaga acttggcagg agaagagtgt ttacatcggt tcatgaaatc
     2521 gatgaaacat tggatttagc agtaatactc gttcccggaa gatttgtccc tgaaaccctc
     2581 atggcatgcg ccaaaaaggg tatccggcat gtgatcatcg agtctggagg ttttacagag
     2641 cttgatgtaa gcaagaaggt gctggaagat caaatacagg ctatctctga acagcatact
     2701 atgcaggtca ttgggccaaa ctgttttggg gtcatcaatc tggaagccgg tgttgtgcta
     2761 ccctttttcg tcctcaatcc cggatacatg aaaaaaggta ctgtatcact tgtctctcaa
     2821 agtggtggta ttttttacga tacatccatg cttgcttcat gcgagggtgt tggactgaac
     2881 aagctgatca gcatcgggaa taagcttctt accgatgaaa tcgtctgcct cgaatacctt
     2941 atcaacgata aaaaaactgc cattatcggg ctctatcttg aaaattttat caacggtaga
     3001 cgtctcatgg acctcgccgc tcagactacc aagcctattg tgtgcctcaa ggccaatcga
     3061 agtcctgctg ctgaagaggt tgcccggttc cacacaacag cacttgcagg ggatgacagg
     3121 attgccgacg ctgcacttgg acaggctgga atccacaggg tacaaaattt tcgtgaaatg
     3181 atggatgcct tcaaggtgtt cagcctccct atcataaaag gaaacaggct tgcgctcatt
     3241 acccgttcag ggggacacgg tgttttgtcc ggtgatgcaa cccaccgcta cggctttgtg
     3301 cctgctagcc tatcagaaag tttttttgat cttgtcaaaa gcaagaagat aaatgtcata
     3361 cggacgacaa accctgttga tgtaggagat gtttacagcc tgaatgtcta tggtgaaatt
     3421 tttgaaaaag cactccagga agagaatgtc gacggggtga tctttgtggc aacattcagt
     3481 tctgagacag acagcgttgc agtggagaag gctttaaaag atgctgccag gttaacacca
     3541 atatatggaa aaccaatcgt gttctgcatg gtcaccaata aagaccgctg gtttcatctt
     3601 aaagaagcag ctgatttccc tgtgttcacc gatgtggacg atgccctgaa agcacttgcc
     3661 ttgtcccgtg aacattggaa taacctgggc aaaccgactc gtacagggaa aacaatgtct
     3721 ttcctccgcc acaaaaaggt cttaaaaaat atgaatgcac cgggactgct cgatagtaca
     3781 cacagttttt ctcttgtgca agaatatgga atcgctgtgg cagaatattc tctagcgaca
     3841 acccattcag aggccatgga aaatgcacga aaaatcggct atccagtggc attgaaaact
     3901 acctctccat ccgttctcca caagacagag cacaacgggg tatttctcaa cataaaaaat
     3961 gacagggaat taagcaaggc cctgggcaaa ataaaaacag acacctattt tattcagaaa
     4021 atggctcctg ccggaactga ggtgattgtg ggtggcaaac gcgaccgtga attcgggccg
     4081 gtcatcctct tcggcctggg agggatcttt gtcgaagtat tccaggatac ccttatcagg
     4141 gttgccccgg tcgacttggt tactgctgcg cagatgatcg attcaatcaa tggttcacgc
     4201 ctcctcaaag gatttcgtgg aagcaagcct tctgatattg aggtgcttgc ccatgttctc
     4261 gtaaacatct ccacgctcct catggatcac cctgaaatca cagaccttga cattaatccg
     4321 cttatcgtcc tggaaaaagg cagaggctgc attgctgtgg atgtaaagat tcaaatctct
     4381 taaccatcca actcaataat tgacgtgttc aaaagtgaaa cgtcaaagat cataaaaaga
     4441 caggggcccg tgattccatg aattgtttca gataaagaga aattgcatgt gcagctagat
     4501 ttgtcagcat aaccctgtca gcatgctggc tttaaaattt caggcaggac aggtggcctt
     4561 taatcctgcc tcaggttaca atagcacccc taatcagcaa attgtgatta tcaatcaagg
     4621 tgagattatg caaattattc agtgtacatg gggatttatt ccctcatgga cgaaggagcc
     4681 tgttattggc tataccatga ttatgccagg tccgggactt tagttgagaa aaaggagaaa
     4741 cacggacagt tcagtttact gtgcgatcct ttcccctatc attgaatatt tttctgcaaa
     4801 aggggtgtcg ccttctctca caaaagctgt gccctccatt gtatcccctt ttaccaccgc
     4861 ctccatgttt ataatgaaaa cccttcctcg tacagcatcg gagacagtca tggcaatatc
     4921 attgccctct atatttgtgt cttccacatt gtatcgtctt ttttcatcac tcagagtagc
     4981 ccgaatcttt tgaaaatcct gtttaatagc gagcttgtat ccccgagact cagtctgttt
     5041 attgagcgtt atccgccagt tcccgtcaac ccaggcaggg acgacccaca ggtgtaccgt
     5101 tgtgccgtac gccttcaaag tcatgtccgg tttccatttc cccatctccc acatgtggga
     5161 aacaatcttt gatgctggct tcaattcttt cagaaacttg ggcagcaagc gggcatttaa
     5221 atccggaaga aggtaaaggg taacaacagt cgcatcatgc agatcaactg taaagaaatc
     5281 ttcctcaaga aactttacct tctcagtaac accggcttct ttggcgtttt taacgctttc
     5341 cccaataagt tccttatcga gctcaacccc gacaccacgg gcaccgaaat cccttgctgc
     5401 tgcaattaca attcggccat cacccgatcc aaggtcgtag acagtatcat ccctgctaac
     5461 ttcagcaagt ttgagcatct ctttaactac cttatgtggt gtggttgcaa aatggacatc
     5521 aggtatagtc ttcttgccag ggatggagtc acatgaaaac aggacaaagc aaaaagcaag
     5581 aatagaggca atatggacaa cccttctttt catcggtcta cctcctttta gtgacgtggt
     5641 gttttgaaag cttcataaaa aaagtgcctg ttaaaaacac ggctatccct gttaacgcac
     5701 caatgcctgt atactggatg cttgacacaa gcatatagat acatatagca caaaacaata
     5761 acggagtaac aggatagccc ggtacgacaa aaggtcgttt tgcctctggt tctttcaagc
     5821 gcaggattat gatagaaagt gtcgtaagaa aaaagaagag ccagaagatg gatgctgtgt
     5881 attcgaccat ggtcacgaag cctttcattg ttattgaacc aagtacaaca agaaaaaagg
     5941 agattgcacc ctggacaatt aatgcattgg ctggtgtgcc agagctttca tgccaggccc
     6001 ccaggaagcg aaaaatggaa aattcagttc ccagggcgta attcgttctc gctcctgtga
     6061 tgatcgtggc attcatcgct ccaagagcag atatggcaat caatactgtg gaaagcactg
     6121 ctccgtaatg acctacaatc cgaaccatta catcgaaaac gattgcatct gactttccca
     6181 cagaaacaat gccaaggaca tggacataga cccagttgat aagtacatag atggatgtaa
     6241 tgataagtat accccacaga agtgagtgaa ccattcccct gcctgagcct ttcagttccg
     6301 ctgaaatata ggccgcttca ttccatccgc cataggtgag aagaacaaag atcatggcaa
     6361 ggccaaacac atggccagtc aaatagggtt gctcttgtgc agtcgagaac ggacttgaaa
     6421 aaaatactcc ccctataaca acaattagta aaccaatgat ctttgttaca gtgagaaggt
     6481 tctgggtggt ctttccttcc cgtatgccaa taatattaat cactgtaaga ataattatac
     6541 cggctgcccc atagatggaa gctgagtatg gccccaatga gtatatccgt gacatgtagt
     6601 ctccaagtac aaaagaaagc atggcaatgg agccggtctg aataaccgtc atccttgccc
     6661 aggcaaacag aaaagacacc ctgggaccgt aggcccgtgt gaggtaatgg tattccccgc
     6721 caggatgcgg ataggctgta gcaagctcag cgtagcagag agcaccaaca aaagagatcg
     6781 ctccaccgag aacccaggtc aaaagaaatg tacccacgct gccggcgttt gcagccacca
     6841 gggagggcgt tttgaaaatc cccacgccta ccacaatacc cactataagg gctactacat
     6901 caagaacaga tagggtcttc ttcatatgaa cttaacgttg atgaggaatc ctatccaaca
     6961 gcctgaagtt tgtcaattag aaacttttgt tcaaaaatat gctcagtttt ttatgacgcc
     7021 gcggccatgg tatactctga tttggagtta gaataggtat ggtgtctgca attccctgga
     7081 tcatcttgga tatactcttg acccacatgg ctttttttaa ctcctcaggt catatgttca
     7141 tcccccatcc tgattcatct cgcatggcgt tcatgaggac attatgcagg attatcatag
     7201 gctatggcca caactctggg agcagtggcc ctttttcttt cctacaatac aaaggaaata
     7261 taaatggcac ttgtaactgc ggatgatagg ttagcagggg ttcacagggc tattcttttt
     7321 ccttgatttg tctgctaccg tggagtaatg tagtcggcag ggtcgcatga aatcgaatcg
     7381 tatagttttt tttgtactcg gctggatcgg aatgcttctc tatttcaccc aacgatggat
     7441 attcggcccc ctcattcctc cgcttatgga ggagtttcat acagacagga ccgctcttgg
     7501 tattattgga gctgcatctc tctggggcta tatgctcacg cccattgcag ccggcgtact
     7561 gtcggacagg ttcggcagga aatgtgcgat tattattgga attttcggat tctctctcct
     7621 tacggtagtg tgcggatttt cgaacggtcc acgctttctc tttttcggtc gttttctcac
     7681 aggcatggtg gaagctttct atttcattcc catgcttgcc tttaccctgg agctctttcc
     7741 cgaacgacct ggatttttct taactactct ctcctctgga tcctccctgg gatggtttgt
     7801 gggtccagca ttatccggat ggctcctcga tctgacaggt agttggcgtg tacccttctt
     7861 tgtggcgggt ctggcaggca tcctcgttgc aatcttctta cttacgacat ggcctgatcg
     7921 cgacaaggcc agcaccacaa tcagatactt tgaccgtgct atcctgaagc gtgcgaacct
     7981 ggttatgctt gtatttctca gtctcaccgg tatgtttcag atttccgccg aatttggctt
     8041 caccatgtgg tatcccgctt ttttaaaaac agaactcgga tttgcagcca ccgccgccgg
     8101 tctcgtagct ggtctctatg gcataggcca gtgtatcggc cggccaattc tcggctgggt
     8161 atccgacaga ctcggctaca gacgggtggg aatcttctgt gcattcatta tggggataac
     8221 actgatgatt atcctcgcca caaacaatcc tgttctcaga gcgtactgta ccttccaggc
     8281 tggatttatt ggggcagcaa tgatgggcgc ggtatggaca tttaccggcc tcgtttttcc
     8341 aacatttaaa gggctcgccc tgggtgtgat cacaaccttc gggtatgcca cggcatctct
     8401 agcccccata tcaataggct atattggtga tcactattca atttccacag ggctctgggt
     8461 cattgccgtt cctgcggtat ttcttggcgc cttatcgctc ctgtccacat tcctcattcg
     8521 acccaccacg aaaaggtaga cccgttctcc a
//
LOCUS       DAOOMC010000119         8254 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdus sp. isolate AMR_MDS_2685
            NODE_10182_length_8254_cov_5.802171, whole genome shotgun sequence.
ACCESSION   DAOOMC010000119 DAOOMC010000000
VERSION     DAOOMC010000119.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864760
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdus sp. (wastewater metagenome)
  ORGANISM  Syntrophorhabdus sp.
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae; Syntrophorhabdus.
REFERENCE   1  (bases 1 to 8254)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8254)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,729
            Genes (coding)                    :: 2,708
            CDSs (with protein)               :: 2,708
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 7 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8254
                     /organism="Syntrophorhabdus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10182_length_8254_cov_5.802171"
                     /isolate="AMR_MDS_2685"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2699756"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(236..574)
                     /locus_tag="PK125_11070"
     CDS             complement(236..574)
                     /locus_tag="PK125_11070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB38685.1"
                     /translation="MTTLDDWKKMAEDGLKALKETAQDLAFNVEKQAKLGKKKYLDIA
                     KIQRAIDKLLIEIGEYTYDEVTSGRNLSKDDPFIKEKTSSITHMRSEIDLIEEEIHEI
                     QLARPPKPEP"
     gene            825..1868
                     /locus_tag="PK125_11075"
     CDS             825..1868
                     /locus_tag="PK125_11075"
                     /inference="COORDINATES: protein motif:HMM:NF002327.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016793 - triphosphoric monoester
                     hydrolase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyguanosinetriphosphate triphosphohydrolase"
                     /protein_id="HPB38686.1"
                     /translation="MNIRLKLEEREELILGPFAQRSGKTRGRLREETECDIRPAFQHD
                     RDRITHSKAFRRLKHKTQVFLTPAGDHYRTRLTHTLEVAQIGRTIARALALNEDLVEA
                     ISLGHDLGHTPFGHAGEEVLNRIHAGGFRHYEQSLRVVDILEKEGQGLNLTAEVRDGI
                     LKHSKGKGSVVIQDDCDKPLTKEAEVVRVADIIAYINHDIDDAMRGNVIHDNDLPRES
                     IKYLGETSSDRIDAMVRGVIQETLKNIDARISVGAELEHHMVTLRDFLYDRVYDSVTV
                     HQDFLKCSRIIEDLYTYFLRNKDAFLDETKKNEYYDDPAVCVCDFIAGMTDRYAFNLF
                     EKIFLPLPWKMQI"
     gene            1972..4608
                     /locus_tag="PK125_11080"
     CDS             1972..4608
                     /locus_tag="PK125_11080"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF004349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HPB38687.1"
                     /translation="MMEKIYNPHIIEGKWYQFWIDRGFFTAHNGSEKPPFSMVIPPPN
                     VTGSLHMGHALNNTLQDIMARYKKMCGFNSLWVPGTDHAGIATQNVVERELAKEGLDR
                     ETLGREKFIERVWKWKEQSGGIIIEQLKRLGCSCDWSRQRFTLDEGLSRAVREVFVRL
                     YNEGLIYRDNYIINWCPRCKTALSDLEAEHEETQGYLWHIKYPLADGSGYLTVATTRP
                     ETMLGDTAVAVNPQDERYSKYIGQYVILPIVDKKIPVIGDSYVDTSFGTGVLKVTPAH
                     DINDFEIGKRQNLAIVKVIDDDGKMNENAVHYHGMDRFECREKIVAELTGKGLLEKVE
                     PYTLGVGKCYRCKTVVEPSLSLQWFLKMRPLAEPAITAVRDHKIRIIPEMWEKVYFEW
                     MENIRDWCISRQIWWGHRLPVWYCDTCNKTYVSVEDITTCDGCNGVLRRETDVLDTWF
                     SSGLWPFTTLGWPEHTKDLQVFYPTALLITGFDILFFWVARMIMMGIKFMGDVPFREV
                     YIHALVRDAEGKKMSKSKGNVIDPLLMIDEYGTDAFRFSLAMLAAQGRDILLSEERIE
                     GVRNFVNKIWNASKLSLSFIGNSNYKEFPARSSFLPDQWIRSRSQKMVRDVSDSIEGY
                     RFNEAANVIYNFVWKEFCDWYLELIKPNLYGKVTTFDKESTKATLHKTLSEILKVLHP
                     LMPFVTEEIFQRIPGKTEDTIMAATFPRFDESECDEDAENRMDLIMGVIDVVRNIRGE
                     TGIAPNVKVDVVIRSDSNQSLLKEYGYYIKELAKIAHLSFTDGAVPDQSAVGVYREVE
                     IFVPLKNLIDVSKELSRIDKELFKIDEDCEKVKQKLNNPSFMKKAPEEVIEKNQSHYE
                     DLQKKRKKLIISKNLLENLSGN"
     gene            4609..5916
                     /locus_tag="PK125_11085"
     CDS             4609..5916
                     /locus_tag="PK125_11085"
                     /inference="COORDINATES: protein motif:HMM:NF015410.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative nucleotidyltransferase substrate
                     binding domain-containing protein"
                     /protein_id="HPB38688.1"
                     /translation="MALNLITLENFLAENKERMHDKALLRHERYRILSKLRDKLQHSL
                     DDYIRFESELSSLVVDKIASSKTFQDLLLCHKRAVAGIEKYFFEEDTIVDVHDLFRIV
                     RDGLTIRVIELVEAEMENEGYGYPPTNFVWVGMGSEGRDEQTLLTDQDNMVLYGEVED
                     EFATDPLKNACYEYLKNDKGVGNPKEVTKKQLLDYYFKVFAEKMVERLHEVGFEKCKG
                     GVMPSNEKWRGSIDQFRDRLIQRITYEKGIFETLDIIILTDARPIYGDKNLLNSFRQE
                     FFKILIDNKAVMKDLYESAVLMPTALSFFGNFKTEKSGEHKDMINIKLLGWSPLILAV
                     RMVAITNEIFETNTLKRVKLLREKNVIRKEMEADLIDAYLIFVKFRLQSQSNNKDSNQ
                     ANMNFLFPRNLSDDEQSKLRKAMRTVESFQKFIQERLLFGQPI"
     gene            5927..6298
                     /locus_tag="PK125_11090"
     CDS             5927..6298
                     /locus_tag="PK125_11090"
                     /inference="COORDINATES: protein motif:HMM:NF012780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HPB38689.1"
                     /translation="MKVVELMNKNVTTCHPDEKLSVIVNKFELFNIAGMPVVEKGKLV
                     GIVCQTDILKGLKTGNMQNLTVKDVMVTNVIFASPTESVVTVAKIMVEKHINRIPIVE
                     NDSLVGIVTRGDIIKAVAESG"
     gene            6354..6650
                     /gene="gatC"
                     /locus_tag="PK125_11095"
     CDS             6354..6650
                     /gene="gatC"
                     /locus_tag="PK125_11095"
                     /inference="COORDINATES: protein motif:HMM:TIGR00135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HPB38690.1"
                     /translation="MKITREVVEYVAHLGRLDLESHEVDLYTNQLDRILEYIDKLNTL
                     NTEGIEPTGHAMIAQCLLREDVVKDSFDVEESLRNAPERKGNFFKVPPIIELEG"
     gene            6735..8210
                     /gene="gatA"
                     /locus_tag="PK125_11100"
     CDS             6735..8210
                     /gene="gatA"
                     /locus_tag="PK125_11100"
                     /inference="COORDINATES: protein motif:HMM:TIGR00132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HPB38691.1"
                     /translation="MENLLDMTISEMRALLKKREITAVELASFYLKRIERYDNDVMAY
                     LNVTEDKAHEMARIADRKMEQGEDGPLLGIPFGIKDIFCTKGIETTCASQILKGFMPP
                     YDATVIKKLKDASFVHLGKLNMDEFAMGSSTENSSFHITKNPWDLTRIPGGSSGGPAA
                     AVAAGLCAAALGTDTGGSIRQPAGLCGVVGMKPTYGRVSRYGLIAFASSLDQVGPLTR
                     NVSDCAIVMNEIAGFDSMDSTSIPQPVPDYTENLGKDIAGLRIGIPKEYYIDGMDSHV
                     KKAMEESLSIFEKQGAILIDISLPHTEYAVATYCVIGTAEASSNLARYDGVKYGLRTE
                     GKDIADMYKKTRLKGFGKEVKRRIILGTFVLSSGYYDAYYRKAGKVRTLIRRDFDEAF
                     KSCDILITPVSPTTAFRIGEKTEDPLTMYLSDMFTIPSSLAGLPALSLPCGFDDNSLP
                     IGLQIIGRPLDEALMMQAAYVFESVKNIEKIPGAFRKEYLL"
BASE COUNT         2557 a         1441 c         1972 g         2284 t
ORIGIN      
        1 aaggatgaat cagtgccatt tggattgaac atgtgggtgg tggctgataa tatccgcaag
       61 ggtgcagcgt tgaataccgt tcaaattgca gaagtcctgg cacaaaagta tctttagata
      121 aagaaagact tatggtactg tgaacatcca tgagctttgt gctttcaatt ggttttttta
      181 atttttgacc aattagttcc ttaatcctga gtttggggta gcattgagtc gcttgttatg
      241 gctctggttt tggtggacgg gctaactgga tttcatggat ctcctcctca atgaggtcaa
      301 tctctgaacg catgtgagta atggaggagg ttttttcttt gatgaaagga tcatctttac
      361 tgagatttct ccctgacgtt acttcatcat aggtatactc tccgatttca atgaggagtt
      421 tatcaatggc acgttggatt ttggcaatgt caagatactt ctttttgcct agttttgcct
      481 gtttttcaac gttgaatgca aggtcctgag cagtttcttt taaagctttt aagccatctt
      541 cagccatttt tttccagtca tctaaagttg tcatcactcc tcccatggag tattcaatta
      601 tggtaaaata tacttgtaat tcaccctttt ttcaagtatt taggtgtaat caaaacaagt
      661 ttccgaatct attgggcgat ttggaatgac atttaaatgc ccctcctcag gtaagaggct
      721 ataagtatta gcttattgaa atacctatac tcatgtgata gattacgaac aaccatgtac
      781 aatggttatt gtcaattcct tttctttgtt tgcggataac aagcatgaat ataaggttaa
      841 agttggaaga aagagaggaa ctcatactgg gaccctttgc gcagaggagt ggaaagacaa
      901 gaggacgact gagagaggag acagagtgcg atattagacc ggcctttcag catgatcgtg
      961 acaggataac tcatagcaaa gcattccgaa gactcaagca caaaacccag gtctttctta
     1021 cacctgctgg cgatcattac agaacgaggc tgactcatac ccttgaggtg gcccaaattg
     1081 gaaggaccat tgcaagggcg cttgctttga atgaagatct tgttgaagcc atttctcttg
     1141 gccatgacct cggacataca ccttttggac acgcaggtga agaggttttg aatagaattc
     1201 atgcaggagg cttcaggcat tatgagcaga gtctcagagt ggttgatatc cttgaaaagg
     1261 aaggacaagg ccttaatctt accgctgagg tcagggatgg gatattgaaa cattctaaag
     1321 ggaagggaag tgttgtcatt caagatgatt gcgacaaacc ccttaccaaa gaagctgaag
     1381 tggtcagggt tgctgacatt atagcctata taaaccacga tattgatgat gcaatgcggg
     1441 gaaatgtgat tcatgacaat gatctgccgc gggaatctat aaaatatctt ggtgaaacca
     1501 gttcggatag aatcgatgcg atggtcaggg gagtgattca agaaaccctg aaaaacatag
     1561 atgcaaggat cagtgtgggt gctgaacttg aacatcacat ggttacgctt agagattttt
     1621 tatatgatag agtctacgac agtgtaacgg tccatcagga ttttttgaag tgttcaagga
     1681 tcattgaaga cctttatact tattttctcc gcaataagga tgcttttctc gatgaaacca
     1741 aaaagaatga atactatgac gatcctgcag tgtgtgtgtg cgattttata gctggtatga
     1801 ctgacagata cgcattcaat ttgtttgaaa aaatattcct gccgttgcca tggaagatgc
     1861 agatatgaaa acagtgtgtt agcagaaagc agtcatcagt gagcagcaga actatatata
     1921 gtcacgataa aaactggatt gtaaacttgt accgaaagga attggaaaag gatgatggaa
     1981 aaaatatata accctcacat tattgaggga aagtggtatc agttctggat tgacaggggt
     2041 ttttttaccg ctcacaatgg atctgaaaaa cccccgtttt caatggttat ccctccgcca
     2101 aatgtgactg ggtcgcttca catggggcat gccttaaaca acaccttgca ggatattatg
     2161 gcaagatata aaaaaatgtg tggattcaac tcgctctggg tgccgggcac tgatcatgca
     2221 ggtattgcca cacaaaatgt tgttgaacga gaactggcaa aagaaggtct agatcgtgaa
     2281 acactgggtc gtgagaagtt tattgagcgc gtgtggaagt ggaaagaaca atcgggtgga
     2341 atcatcatag agcaactcaa aagactcgga tgctcgtgcg attggtccag gcaacgattt
     2401 accctcgatg aaggtttatc gagggcagta cgggaagtat ttgtcaggct gtacaatgag
     2461 gggttgattt acagggacaa ctatatcatc aattggtgtc cacgatgcaa aacagcccta
     2521 tcagacttgg aggcagagca cgaagaaacg cagggctatc tctggcatat taaataccct
     2581 cttgcagatg ggtccggtta tctgacagtg gcgacaacga ggcctgaaac catgctgggt
     2641 gatacagctg ttgcagttaa tcctcaggac gaacgataca gtaaatatat tggccaatat
     2701 gtaatacttc ctattgtcga taaaaaaata cctgttattg gcgattcgta tgtggatacc
     2761 tcattcggca caggcgtact taaagtaaca cctgctcatg atatcaatga ctttgaaatt
     2821 ggcaagagac aaaaccttgc aatagtcaag gtcattgacg atgatggaaa aatgaatgaa
     2881 aatgctgttc attatcacgg gatggacaga tttgagtgtc gtgaaaaaat agttgcagaa
     2941 ctcactggta agggtttact cgaaaaggtg gaaccttata ccttaggcgt gggcaaatgc
     3001 taccgttgta aaacagtggt tgaaccaagc ctgtctctcc aatggttttt aaagatgagg
     3061 cctcttgcgg aacctgctat taccgcggtt cgtgatcaca agatcaggat aattcctgaa
     3121 atgtgggaga aagtttattt tgagtggatg gaaaacatcc gggattggtg tatttcacgg
     3181 caaatctggt ggggtcatag actgccagtg tggtactgtg atacatgtaa taaaacctat
     3241 gtttcagtgg aagatatcac aacttgtgat gggtgtaatg gggttttacg aagagaaacg
     3301 gatgttcttg acacatggtt ttcatccgga ttatggcctt ttacaacact tggttggcct
     3361 gaacacacaa aagatctcca ggtgttttac ccgacagcac tcctgatcac cgggtttgat
     3421 attcttttct tttgggtcgc taggatgatc atgatgggaa taaaattcat gggggatgta
     3481 ccattccgtg aggtttatat acacgccctt gtccgtgacg cagaaggcaa aaaaatgagt
     3541 aaatctaaag gtaatgtaat tgatccgctc ctgatgatag atgaatacgg gacagatgcc
     3601 ttcaggtttt ctctcgccat gcttgcagct cagggaaggg atattttgct atctgaagag
     3661 cgcatagaag gtgttagaaa ttttgtaaat aaaatatgga atgcatcgaa actgtcccta
     3721 tcatttattg gcaattctaa ctacaaggag ttccctgcaa gatcttcttt ccttccagat
     3781 caatggattc gttcacggag ccagaaaatg gtgcgcgatg tgtctgactc aatagaagga
     3841 tatcgattca atgaggcagc aaatgtgatt tacaattttg tttggaagga attttgtgat
     3901 tggtacttag aactgataaa gccaaacctg tatgggaaag tgacaacgtt tgataaagag
     3961 tcaacgaaag caactcttca taaaacattg tctgagattc tcaaagtgct tcatccgttg
     4021 atgccttttg ttacagaaga aatattccaa aggatacccg gaaaaacaga ggatactata
     4081 atggctgcca cttttcccag atttgatgaa agtgagtgtg atgaggatgc tgagaaccgt
     4141 atggatctta tcatgggtgt catagatgtg gttcgaaata ttagaggtga aacgggcatt
     4201 gcacccaatg taaaagtcga cgttgtgatc cgtagcgaca gcaaccagtc gctcttgaaa
     4261 gagtatgggt attatataaa agaacttgca aagatagccc acctatcttt tacagatggt
     4321 gctgtcccgg atcagtcagc tgtgggggtg taccgtgagg tcgagatctt tgtaccgttg
     4381 aaaaatctta tcgatgtctc gaaagagcta tcgagaatag acaaagagct atttaaaatt
     4441 gatgaagatt gtgaaaaagt gaagcaaaaa ctgaataatc cttcgtttat gaaaaaggct
     4501 cccgaggaag tgatcgagaa aaatcaatct cattatgagg atttacaaaa gaaacgaaaa
     4561 aagcttatta taagtaaaaa cttattggaa aacctttcag ggaattaaat ggcactcaac
     4621 cttattaccc ttgagaattt tttagctgaa aacaaagaac ggatgcacga caaagctttg
     4681 ctccgtcatg aaagatacag aatcctgtcc aagttgaggg ataaacttca acattccttg
     4741 gatgattata tccgatttga gagcgagttg tcctcccttg tagttgacaa aatagcaagt
     4801 tcaaaaacct ttcaagacct gcttttatgc cacaagcgtg ctgtagcggg catagaaaaa
     4861 tatttttttg aagaagatac aatcgtagat gtccatgatc tctttcgaat agtgcgggat
     4921 ggtttaacta tcagggtaat cgaacttgta gaagcagaaa tggaaaacga aggatatgga
     4981 tatcccccga caaattttgt ctgggtagga atgggtagcg aagggcgaga tgagcaaacc
     5041 ctcttgacgg atcaggacaa catggttctg tatggagaag ttgaagatga atttgcaacg
     5101 gacccactca aaaatgcatg ttatgaatat ttaaaaaatg ataaaggagt tgggaatcca
     5161 aaagaagtaa ccaagaagca actgcttgat tattatttta aggtctttgc agaaaaaatg
     5221 gtggaacggc tccatgaagt cggttttgaa aagtgcaaag ggggtgtaat gccctccaac
     5281 gaaaaatggc gcggttccat agaccaattt cgtgacaggt tgatacaaag aataacttac
     5341 gaaaaaggaa tctttgaaac cctcgacatc ataatcctaa cagatgcaag gccgatatat
     5401 ggtgacaaaa atttattgaa cagctttaga caagaatttt tcaagatatt gatcgacaat
     5461 aaagcagtta tgaaagatct ttatgagtca gctgtactta tgccaactgc cttgagtttt
     5521 tttggaaatt tcaaaacaga gaaatccggg gagcacaagg atatgatcaa tatcaaattg
     5581 ctcggatggt cgcccttgat tttggcagta cgcatggttg caataacaaa cgagattttt
     5641 gaaacgaaca ctttgaagag ggtcaaactt ctgagagaga aaaatgtaat tagaaaggag
     5701 atggaagctg atctcataga tgcgtatttg atttttgtaa agtttcgcct ccaaagtcaa
     5761 agcaataata aagatagtaa tcaggcgaac atgaatttct tgtttccgag gaatttaagc
     5821 gatgatgaac agagcaaatt gagaaaagcg atgcgaactg tggagagttt tcagaagttc
     5881 atacaggaac gcttgttatt tggacaaccg atttaaggag ggatggatga aagttgtgga
     5941 gcttatgaac aaaaatgtga caacttgtca ccctgatgaa aaattaagcg tcatagtaaa
     6001 caagtttgag ctgtttaata tagcaggcat gcccgtggtg gaaaaaggga aactcgtggg
     6061 cattgtttgt caaactgata tattgaaagg cctaaaaact ggtaacatgc agaatttaac
     6121 agttaaggat gtgatggtaa cgaacgttat tttcgcttcc cccacagaat ctgtcgttac
     6181 tgttgcaaaa ataatggttg aaaaacacat caacaggata ccaatcgttg aaaacgacag
     6241 cttagtaggg atagtgactc gaggagacat cataaaagct gtggcagagt ctggataggt
     6301 atggaagtat cagtttataa tcccgtgaag gggaagtaaa atctgaggag ggtatgaaga
     6361 taacaagaga agtggttgag tatgttgcac atctcggaag gcttgatttg gaatctcatg
     6421 aggttgatct ctacacgaat cagcttgata ggattcttga gtatatagac aaacttaata
     6481 cactcaatac tgaaggtatt gaaccgacag gtcatgcaat gattgcacaa tgcctgctac
     6541 gggaggatgt tgtcaaagac tcctttgatg tagaggaatc actacgaaac gctccagagc
     6601 ggaagggaaa tttttttaaa gtacccccga tcattgaact tgaagggtaa tgatccagcg
     6661 ataatgcata gggcttaaga gtgaagaggt caagcttgta ctctaagctg tcgtagaatt
     6721 gtgaggagga cagtgtggaa aatcttctcg atatgacaat atcagagatg agagccctct
     6781 taaaaaaaag agagatcact gctgtggaat tggcatcttt ttatctgaaa aggatagagc
     6841 gatatgacaa tgatgtaatg gcatacctta atgtcactga ggataaggcg cacgaaatgg
     6901 cccgcatcgc cgatcgtaaa atggaacagg gagaggatgg cccccttttg ggcataccat
     6961 ttggtattaa agatattttt tgtaccaaag gtattgaaac tacctgtgcg tcccagattt
     7021 taaagggttt tatgccgcct tacgatgcaa cagtaataaa aaagcttaag gatgcatcgt
     7081 ttgtgcacct tggaaaactt aacatggatg agtttgctat gggatcctct actgagaact
     7141 cgtcatttca tataacaaaa aatccatggg atttaactcg aataccaggc ggttcaagcg
     7201 gcggcccggc agctgccgta gctgccgggc tttgtgctgc tgccctgggc acggacacag
     7261 gaggttcgat cagacagcct gcaggccttt gtggggttgt tggtatgaaa ccaacctatg
     7321 ggagggtttc ccgttatggg ctgattgcat ttgcttcttc ccttgatcag gttggtcctt
     7381 tgacaagaaa tgtttctgat tgtgcaattg taatgaatga aatagctggt tttgattcca
     7441 tggattcgac atcaattccc caaccggtac ctgattatac agagaatttg ggtaaagata
     7501 ttgcagggct tagaataggc attccgaaag aatactatat tgatggaatg gattctcatg
     7561 tcaaaaaagc aatggaggaa tccttatcga tatttgaaaa gcagggagcc atcctcatcg
     7621 atatttcttt accccatacg gaatacgctg ttgcaacata ttgtgttatt ggtactgccg
     7681 aagcatcttc aaatctggct agatatgatg gtgtaaagta cggtttgagg actgaaggca
     7741 aggatattgc cgatatgtac aaaaagacac ggctgaaagg ctttggaaaa gaggtaaagc
     7801 ggcgcattat tctgggtacc tttgttttgt catctggtta ctacgatgca tattaccgga
     7861 aggcggggaa ggtgagaacg ttaatcagga gagattttga tgaagctttc aaaagttgcg
     7921 acatacttat aacacctgta tcgccaacaa ctgccttcag aataggggaa aagacagagg
     7981 atcctttgac catgtatctc tcagatatgt ttactatacc aagcagcctt gccggcttac
     8041 ccgcactatc tctcccctgc ggatttgatg ataatagttt gcccattggg ctacagatca
     8101 ttgggaggcc actggacgaa gcactcatga tgcaagcagc atatgttttc gaaagcgtca
     8161 agaatataga aaagataccg ggtgcttttc gaaaagaata tttgttatga aatttccagt
     8221 tacagatcga actgtcagag gttttctgtt agca
//
LOCUS       DAOOMC010000120         8241 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdus sp. isolate AMR_MDS_2685
            NODE_10201_length_8241_cov_5.748473, whole genome shotgun sequence.
ACCESSION   DAOOMC010000120 DAOOMC010000000
VERSION     DAOOMC010000120.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864760
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdus sp. (wastewater metagenome)
  ORGANISM  Syntrophorhabdus sp.
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae; Syntrophorhabdus.
REFERENCE   1  (bases 1 to 8241)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8241)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,729
            Genes (coding)                    :: 2,708
            CDSs (with protein)               :: 2,708
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 7 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8241
                     /organism="Syntrophorhabdus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10201_length_8241_cov_5.748473"
                     /isolate="AMR_MDS_2685"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2699756"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(363..1448)
                     /locus_tag="PK125_11105"
     CDS             complement(363..1448)
                     /locus_tag="PK125_11105"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HPB38692.1"
                     /translation="MKIFKRSKENRQKKCFGAWQIEITTRCGLQCTMCIKTACKEWHR
                     KDMNARDFEKIVPYLYDVKSVVLEGWGESLLHKNLVDFIRLAKSAGPDVGFVTSGIGL
                     TREYARQIVDAGVDFMGFSLSGATAKTHNSIRVNSDFNTLMASIKNMKELALSSPLEK
                     PKLHIVYLMLKDNIEELPILIDLASKMGINEIVLLNIIQVTSCDQDSNKAFTCEQGSL
                     YKNIMAGALNKAKKLGIYLSMPAVLPQNMAVCSENPLGNIYISVNGEVSPCVFLNPPV
                     SAPFTRIFCGTEYQTFSVSFGNIFTESLDSIWSRKEYKEFRDAFISRQRKWKESYTGL
                     LELRRPDNSPIPDPPAVCKTCHKMLGF"
     gene            complement(1452..2291)
                     /locus_tag="PK125_11110"
     CDS             complement(1452..2291)
                     /locus_tag="PK125_11110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB38693.1"
                     /translation="MISQEELNRTYDHACVPEHIVDYVVSMSEAEPYLIQHYLCYKKD
                     GVLIFIGYPLGIAFNQKQMETSLNMALKAIKPRIVSVIAPTTVTMNGRVIQQNSDSYY
                     RLDLENLVIPSKVRNMIRRASPEISVEHNDQWHEEHEALVAEFLATHKISAESQRIFI
                     KISEYVSSSKSVTILNARNNEGLLIAFDVADYGSRNYAFYMFNFASRKHHVPGVSDLL
                     LSEIIESARRKGKRYINLGLAINNGVMFFKQKWGGAPFLNHEFMLYEISRAGIIESLL
                     SRL"
     gene            complement(2288..3118)
                     /locus_tag="PK125_11115"
     CDS             complement(2288..3118)
                     /locus_tag="PK125_11115"
                     /inference="COORDINATES: protein motif:HMM:NF014066.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HPB38694.1"
                     /translation="MITFREKKLKMVWEMSKDGFTSFLVGTAHFFPYSFKESLIHLIS
                     KADVTLFEGPLDENNMEAVREYGVARQDGMSLYDSLDEKTIAELNKELGESAEFSGCS
                     LSSYISVFKREHRDLLSQEIGGLRPWMAFFKLWSHYLAKRGWKHSVDLEALSVSRELG
                     KKVVFLETIEEQLTALDGIPFERIVTYLSNFRNWGKFSRHHEKQYLKGSLDSLLNIIS
                     GFPTRCPSIVEHRDPVFFTRMRPYVEAGNAVVFLGTTHIQGIVKMLEQEGFKVSQYSK
                     "
     gene            complement(3115..3966)
                     /locus_tag="PK125_11120"
     CDS             complement(3115..3966)
                     /locus_tag="PK125_11120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB38695.1"
                     /translation="MITPDQERWILSKAYVPEHIVSLMTTISKGQPFLSKGHLYYVGD
                     ARGILVGYPLDSEFYSEIFSSIVKDVITCYPGHTWSIIAPRLPAELLSSGVEKQSDFY
                     YSLSFEDFDRKEGLKRIAEKATSRLTVEVTRAMTPAHEAITKEFIEREDPGNFIREFY
                     CAMPDYVLHSDTTLVISARDFDGYLSAFYVLDFAAKDFATYVIGCHSKNHYVPHASDL
                     LFLKMVNMARESGKDYINLGLGVNPGIQRFKKKWGGKPTIPYEFCRQQWGIMGIRTMI
                     DVLLSRL"
     gene            4112..4555
                     /locus_tag="PK125_11125"
     CDS             4112..4555
                     /locus_tag="PK125_11125"
                     /inference="COORDINATES: protein motif:HMM:NF037131.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HPB38696.1"
                     /translation="MDEIDKKILNLIQEEFPLHKRPYKEIGRQVNIDEKEAVKRVQKL
                     KDAGVIRRIGVVFEPKKLDYTSTLCGVHVDEKNLIEVVDVINKHTGVTHNYEREGDLN
                     LWFTIIANKEEEIDAFISDLERRFLLKIYRFPKKRVFKIKTFFPV"
     gene            4664..7240
                     /gene="polA"
                     /locus_tag="PK125_11130"
     CDS             4664..7240
                     /gene="polA"
                     /locus_tag="PK125_11130"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HPB38697.1"
                     /translation="MSEKEEQGLENDERNRRKRIFLVDGNSYLYRAFFATPHLSSSRG
                     IPTNAVYAFMNMIRKLQNDEKPDVLIIIFDSKAPSFREEISKAYKAQRPPMPDNLSVQ
                     IPYVKSILDAMGLPILEKEGFEADDIIGTAVEQLKSEDADFVIVTGDKDMMQLVSPKV
                     KILDTMKGLVIGEKEVEEKFGIKPVHMTDYLALCGDTSDNIPGVPGVGDKTARELVSA
                     YGTLDELYTQIDQVKKPALRERLKAGKDLAYMSKQLATIRLDVPLEAGIDIFRENEPD
                     LKTLRSLYRELEFTALYNEIKIDGEEKKEWKRIDIVALNKRCIALLTNFHGKNVSDIQ
                     LNSFVAFDGHGVLLSQQKDELIDIIRYSKEIITHNLKPLLIYLVSHAFTPLTPVFFDT
                     MLAAYLINPLRKDYGIGSVIEEYLERDFSSNGTEKIMTDAIPYLIELKGVLEKKMEGL
                     GLLSLFMNTEMPLIKVLADMECKGVKIDRSRLNSLSREFDQRLNGIIKKIHDMAGGPF
                     NINSPQQLSRVLFDTLKLTPVKKTKTGYSTDTEVLQTLSTEHPLPKEILEYRTLTKLK
                     GTYIDVLPTLINPLTGRIHASFNQMVVATGRLSSSDPNLQNIPIRGEEGMKIREAFVP
                     EKGHILISSDYSQIELRVLAHISRDQVLIDTFLRDDDIHTRVAQEVFGTQDGGVSHEM
                     RRTAKVINFGIIYGMSSFGLAKELGISQREAQNYIDDYFEKYQGVKAYIKNIQEEARE
                     KGFVQTLFGRIRYIPEIRNNDNNIRQLGERAAMNTPIQGTAADIIKMAMVNIHRIIRE
                     KGFSSSLIMQIHDELVFEVPLEEADVMETLIKTEMEHVVSLSVPLKVSLGKGYSWAEA
                     HD"
     gene            complement(7173..8099)
                     /locus_tag="PK125_11135"
     CDS             complement(7173..8099)
                     /locus_tag="PK125_11135"
                     /inference="COORDINATES: protein motif:HMM:NF015587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HPB38698.1"
                     /translation="MNIGNRIMLKKRKTRTIFEIIKKLFKKYQGDHGNIIVSSISFYV
                     LLTFIPFTLLSIFILGYVIDLSNPGLHLEKLLKTILPDPYNALIAKKVLKELNFISLS
                     KRLSGPLGLLFLFFFTTRLFAVIRPSFQIIFGKTTKSFIRGKGEELFLTLLFSIVQAL
                     LFFSFIFSIMIQTKLGGILPAFLTRGPFVVLFSAFDMVATFVMFFFLYYFLAPVRNKG
                     LILTSTILATMFWYLGRYFFKYFIFHIGRVTAFFGTYGVFIAFLFWIYFSVFVFIVCA
                     ELLSVMIGSPSNRGPQPSYIPFRGSPSKARKD"
BASE COUNT         2499 a         1729 c         1837 g         2176 t
ORIGIN      
        1 tttatttacc ttcaagaaac ccttaacaaa acctctctgt gcagtagagt agattgtctc
       61 ccatacaatg acccagcaag aggagtaaac agagataccc tcgcacaggt gtctgaaaaa
      121 gaaacccttc tacctggaag ttcaatgcgc tggagatggc ggaaccattc tctacgctgg
      181 atctggcggt gcctgagata atagaaaatg aggagagagg ctaaaaactg tatgagcacc
      241 agttactgag acggaattga ggtaaaaact tgtctggact tatggtacag gtgggtgggg
      301 tataactgca tttttaggat aacgccaaga tcatggggtt tactggtggg gtgtatcaga
      361 cttcaaaagc caagcatttt gtgacaggtt ttgcaaacgg caggggggtc aggtatcggc
      421 gaattatcag gacggcgtag ctcgagaagg cctgtgtagg actccttcca ttttctttgt
      481 cgggaaatga aggcatccct gaattcctta tactctttcc tggaccagat ggagtccaga
      541 gattcggtaa agatgtttcc aaagctcacg gaaaacgtct gatactcagt accacaaaag
      601 attcttgtga aaggggccga tactggcggg tttaaaaata cacagggcga tacctctcca
      661 ttcaccgata tgtatatgtt gccgagtggg ttttcactgc atacagccat gttttgtggc
      721 aaaacagccg gcatagatag gtatatcccg agttttttcg ccttattcaa tgctcccgcc
      781 ataatgtttt tatatagaga cccctgttcg catgtaaatg ccttgttact atcctgatcg
      841 cagcttgtta cctggattat gttgagaagt acaatctcat taatgcccat cttggatgcg
      901 aggtctataa gaatgggaag ttcctcaatg ttatctttta gcatcagata gactatgtgg
      961 agctttggtt tttccaaagg actggaaagg gccaactcct tcatattttt tatcgatgcc
     1021 attagtgtat taaaatcaga gtttacccgg atggagttgt gtgtttttgc agttgctcct
     1081 gagagtgaaa accccatgaa gtcgactcct gcatctacta tctggcgggc gtactcacga
     1141 gtaaggccta tgccactcgt aacaaaaccg acgtctggtc ctgctgattt tgcaagacgg
     1201 atgaaatcga caaggttctt gtgaagcaag gattctcccc agccttcgag aactacgctt
     1261 ttaacatcgt aaaggtaggg tacaatcttt tcaaagtcac gtgcattcat atcctttcga
     1321 tgccattctt tacatgcggt tttaatgcac attgtgcact ggagcccgca ccgtgttgtg
     1381 atctctatct gccacgcccc aaaacatttt ttctgtcggt tttcttttga tcttttgaaa
     1441 attttcatga cttacaacct acttaaaagg ctttcaatga taccagcccg tgatatttca
     1501 tagagcataa actcgtggtt aagaaaaggg gctccccccc atttttgttt aaagaacatc
     1561 accccattat taatagcaag accaagattt atatatcttt taccttttct tcgtgctgac
     1621 tcaattatct cggagagtag caagtctgac acgcctggta cgtgatgttt tctggaggca
     1681 aaattgaaca tgtaaaaagc ataatttctc gatccataat cagcaacatc aaaggcaatg
     1741 agtagaccct cattgtttcg tgcgtttaat atggtaactg atttcgagga cgacacatac
     1801 tcagagattt tgatgaagat acgctgtgat tctgcagata ttttatgagt tgcaagaaat
     1861 tcagccacaa gggcttcatg ttcttcatgc cattgatcat tgtgctcaac agaaatctct
     1921 ggcgaggctc tgcgtatcat gttgcgtact tttgatggaa tgacaaggtt ctcaagatca
     1981 agtcggtaat aggagtcaga attttgctgg atgaccctgc cgttcattgt cactgttgtt
     2041 ggtgctatta ccgatacaat gcgcggcttg attgctttta acgccatatt cagactggtc
     2101 tccatctgtt tttgattgaa tgcgatgcct aaggggtatc cgatgaaaat caacactcca
     2161 tcttttttat aacagagata gtgttgaatc aggtaaggct cggcttcaga cattgaaacg
     2221 acataatcta caatatgttc gggcacgcag gcatggtcat aggtacgatt taattcttcc
     2281 tgactaatca tttgctgtat tgtgagactt taaagccttc ctgttcaagc atcttaacaa
     2341 taccctgtat atgtgtagtg cccaggaata ctacagcgtt accggcttcc acgtaaggcc
     2401 gcattcttgt aaagaacacg ggatcacggt gttctacaat agaagggcat cgtgttggga
     2461 atccactgat aatatttaac aaactgtcca gcgagccctt caaatattgc ttttcgtggt
     2521 gacgggagaa ttttccccag ttcctgaaat tgctcagata cgtgacgata cgttcaaagg
     2581 gaattccatc taatgccgtt agttgctctt caattgtttc caggaacact acttttttcc
     2641 caagctccct ggatacggaa agagcttcaa gatcgaccga gtgtttccag cccctttttg
     2701 cgagatagtg ggaccaaagc ttaaaaaatg ccatccacgg ccgcaatccc ccaatttcct
     2761 gtgaaagaag atcacggtgt tccctcttaa aaactgatat gtatgaagac agtgaacacc
     2821 ctgaaaattc cgcactctcg ccaagttcct tattcagttc tgcaatggtt ttttcgtcaa
     2881 gagaatcgta aagggacatg ccatcttgtc ttgccacccc atattctctc actgcttcca
     2941 tattattttc atcaagaggc ccttcgaaca gagtgacatc ggccttactg atcagatgta
     3001 tcagagattc cttgaagcta tagggaaaga aatgggctgt gcccacaaga aaacttgtaa
     3061 acccatcttt actcatttcc caaaccattt tcagcttctt ttcccggaat gtgatcatag
     3121 cctcgatagc agtacgtcaa tcatggtgcg tatacccatg atgccccatt gttgcctgca
     3181 aaattcatag gggatggttg gttttcctcc ccatttcttt ttgaatcgct gtataccagg
     3241 atttacgcca agacccaggt tgatataatc tttacctgat tcccgtgcca tattcaccat
     3301 tttgagaaaa agcaggtcgg atgcatgggg tacgtagtga ttttttgaat ggcaaccaat
     3361 gacgtacgtt gcaaaatctt ttgcggcaaa atccaaaaca tagaaagctg acagatagcc
     3421 atcaaagtct cttgcactga ttacaagggt ggtatctgaa tgaaggacat aatcaggcat
     3481 agcacagtaa aactcccgaa tgaagttgcc agggtcttcc cgctcgatga attctttggt
     3541 gatggcctca tgtgcagggg tcatagccct ggttacctct accgtcagtc gtgaggttgc
     3601 tttctctgca attcgtttaa ggccttcttt ccgatcaaag tcttcaaaag aaagggagta
     3661 ataaaaatct gactgttttt caacaccaga ggagagaagt tctgcaggaa gacggggtgc
     3721 aataattgac caggtatgac caggataaca ggtgatcaca tctttgacaa ttgaggagaa
     3781 gatttccgaa taaaactcag aatccagtgg atatccaacc aggatacccc gggcatcacc
     3841 cacataataa aggtgccctt tggacaaaaa gggttgtccc ttggatattg ttgtcatgag
     3901 actcacgatg tgttcaggca cataagcttt ggaaaggatc cacctttcct ggtcaggtgt
     3961 aatcatctgc ttcacataat tgtagcatac ttgcgtagaa tgttcggagc aattagtttg
     4021 gaattagggg aaagtcagga cctcccaaac atggaaggta ctggctggaa atcttgacaa
     4081 tactatgtgg ggcgtataga atcagggtgc aatggatgaa attgacaaga agatactgaa
     4141 cctgattcaa gaggaatttc ccctccacaa gcgaccttac aaagagattg gaaggcaggt
     4201 gaacattgac gagaaggaag ctgtaaagcg ggttcagaaa ctcaaagatg caggtgttat
     4261 ccggcgcatc ggagttgtgt ttgagccaaa gaaactggat tatacgagca ccctctgcgg
     4321 tgtccacgtt gatgaaaaaa accttataga agtggtggat gtgataaaca aacacacagg
     4381 ggttacccat aattatgaac gggaagggga tcttaacctc tggttcacca ttattgctaa
     4441 caaggaagaa gaaatagatg cttttattag tgatcttgaa aggaggtttt tgctgaaaat
     4501 ctaccgattt ccaaaaaaaa gagtatttaa aataaaaact tttttccctg tttgataatg
     4561 tattattggc catcaatagt caaccgtcag tttgttgcga ttttgtgaat atttttttga
     4621 aagacaatag ctgacttcac caagatgcaa gggagatcga tcaatgtcag aaaaggaaga
     4681 acaagggctg gagaacgacg aacgaaatag acgaaagcgc atcttcctcg ttgacggcaa
     4741 ctcctacctc taccgcgctt tttttgcaac accacacctg tccagttcta ggggtattcc
     4801 cacgaacgca gtctacgcat tcatgaacat gatcagaaag ctccagaatg atgagaaacc
     4861 agatgtgtta ataattattt ttgattcaaa agccccttca ttcagggaag aaatatcaaa
     4921 agcatataag gcccagaggc caccaatgcc agacaacctc tccgtccaaa taccctatgt
     4981 gaagtcaata ctcgatgcaa tgggcctccc aatacttgaa aaagagggtt ttgaggcaga
     5041 cgatatcatc ggaacagcgg tagagcaatt gaaatccgag gatgctgatt ttgtcattgt
     5101 aacaggtgac aaggacatga tgcaactcgt atctcccaag gtaaaaattc ttgacaccat
     5161 gaaagggctt gtaataggcg aaaaagaggt ggaggaaaaa tttggtataa agcctgttca
     5221 tatgacggac taccttgccc tgtgcgggga tacatcggac aacatacccg gggtgcccgg
     5281 cgttggcgac aaaactgcca gggaacttgt gtccgcgtac ggaaccctcg acgaactcta
     5341 cacccaaatc gatcaggtga agaaaccggc cctcagggag agactcaaag caggaaaaga
     5401 tcttgcgtac atgagcaaac aacttgcaac gattcgcctt gatgttcccc ttgaagcagg
     5461 catcgacatc ttcagagaaa atgaaccaga cttaaaaacc ttgcgcagcc tctatcgcga
     5521 acttgagttc acggcccttt acaatgagat caagatagat ggagaagaaa aaaaggaatg
     5581 gaaacggatc gatattgttg ctcttaacaa acgctgtatt gctcttttga ctaatttcca
     5641 tgggaagaat gtgagtgata ttcaactgaa tagcttcgtt gcgtttgacg gccacggcgt
     5701 tctcctttca caacaaaaag atgaattgat tgacatcatc agatacagca aagaaataat
     5761 cacccacaac ctcaagcctc ttttgatcta tctggtttct catgccttta ctcccttgac
     5821 ccctgttttc ttcgatacca tgctggcggc ctatctcatc aatcctctca ggaaggatta
     5881 cgggatcggt tcagtgattg aggaatatct tgaaagggat ttttcgtcca atggtacaga
     5941 aaaaatcatg actgatgcca taccatacct cattgaatta aagggtgttc tggaaaaaaa
     6001 gatggaaggg ttgggcctct tgtccctttt catgaacaca gagatgccgc ttataaaagt
     6061 ccttgcagac atggagtgca aaggggttaa gattgatagg agcaggctga acagcttatc
     6121 aagggaattt gatcagaggc ttaatggtat cattaaaaag atccatgata tggccggagg
     6181 accctttaac atcaattcac cgcaacaact ctcaagggtt cttttcgata ccctaaagct
     6241 gacgccagtg aagaagacaa aaaccggtta ctcaactgat actgaagtgc tacaaacatt
     6301 atccactgaa cacccattac caaaagagat actcgaatac agaacgctca caaagctgaa
     6361 agggacctac atcgatgtcc tcccaaccct gataaatcct cttacaggaa ggattcatgc
     6421 ttcctttaat cagatggtag ttgctacagg acggttgagc agcagtgacc cgaacctgca
     6481 gaacattccc attcgtggag aagaagggat gaaaatacgt gaggcctttg tgcccgaaaa
     6541 agggcatatt ctgatttcat cagattactc acaaatagag ctcagagtcc ttgcccatat
     6601 ttcacgagat caagtcctca tcgatacctt ccttagggat gatgacatac acacaagggt
     6661 tgctcaggag gtttttggta cacaagatgg tggtgtgagc catgagatga gaagaaccgc
     6721 aaaagttatc aactttggca taatctatgg catgagcagc tttgggcttg caaaagagct
     6781 gggcatatcc cagagagagg cgcagaacta catcgatgat tattttgaaa aataccaagg
     6841 tgttaaggca tatataaaaa acatccagga agaagcccgc gaaaagggtt tcgtacaaac
     6901 ccttttcggg agaattcgat atatcccgga gatcagaaac aacgacaata acatacgcca
     6961 gttgggagag cgtgcagcta tgaatacacc aatacaggga acggctgcag acatcatcaa
     7021 gatggctatg gttaatatcc accgaataat cagggagaaa ggattttctt caagccttat
     7081 catgcagatc cacgacgaac tcgtgttcga ggttcccctt gaggaggctg atgttatgga
     7141 gacacttatc aaaactgaga tggaacacgt tgtcagtctt tccgtgcctt tgaaggtgag
     7201 cctcggaaag ggatatagct gggctgaggc ccacgattag aagggctccc tatcatcacc
     7261 gagagtaatt cggcacagac aataaagaca aaaaccgaaa agtagatcca gaagagaaaa
     7321 gctataaaaa caccgtatgt tccaaaaaaa gccgttactc tccctatgtg aaaaataaag
     7381 tacttgaaaa agtaacgccc caagtaccag aacatagtgg caaggatagt tgatgtaaga
     7441 ataagcccct tattgcgaac cggtgctaag aaatagtaaa gaaaaaaaaa cataacaaac
     7501 gttgcaacca tatcgaatgc ggagaaaagt acaacaaaag gaccccttgt gaggaacgca
     7561 ggcaaaatac cacccaattt ggtttgaatc atgatactga agatgaagct aaaaaataac
     7621 aaagcctgga caatggaaaa aagtagggtg aggaaaagtt cttccccctt tcccctgatg
     7681 aaactctttg tggttttacc gaaaataatc tgaaaagacg gccgtattac agcaaagagt
     7741 cttgtggtga agaaaaagag aaagagaagc ccgagtggcc cagagagccg cttggacaaa
     7801 gagataaagt tcaattcctt gagaaccttt tttgcaatca atgcgttgta tggatcaggc
     7861 aatatagttt tgagaagctt ttccaggtgg agaccggggt tgctcagatc gatgacgtag
     7921 ccaaggatga agattgaaag aagggtgaaa ggaatgaatg tgagcagcac atagaaagaa
     7981 atggaggaaa caataatatt accgtgatcc ccttgatatt ttttaaagag ttttttaata
     8041 atttcaaaaa ttgttctggt tttacgtttt ttaagcataa tcctattgcc aatgttcatc
     8101 ctatccgaat gcgcggatct gccactccgt acatgatatc agctaacagg tttccaatca
     8161 atgtaagaaa agctccaatg accaggatgc ccataatagt gggatagtca cgggccatga
     8221 cgctcatgta aaaaagctgc c
//
LOCUS       DAOOMC010000121         8182 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdus sp. isolate AMR_MDS_2685
            NODE_10283_length_8182_cov_6.521718, whole genome shotgun sequence.
ACCESSION   DAOOMC010000121 DAOOMC010000000
VERSION     DAOOMC010000121.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864760
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdus sp. (wastewater metagenome)
  ORGANISM  Syntrophorhabdus sp.
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae; Syntrophorhabdus.
REFERENCE   1  (bases 1 to 8182)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8182)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,729
            Genes (coding)                    :: 2,708
            CDSs (with protein)               :: 2,708
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 7 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8182
                     /organism="Syntrophorhabdus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10283_length_8182_cov_6.521718"
                     /isolate="AMR_MDS_2685"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2699756"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            102..887
                     /gene="fdhD"
                     /locus_tag="PK125_11140"
     CDS             102..887
                     /gene="fdhD"
                     /locus_tag="PK125_11140"
                     /inference="COORDINATES: protein motif:HMM:NF014671.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HPB38699.1"
                     /translation="MSAPDVKGVATDILCSRYSETGWTKAKVHAPLERELTVYVNLQH
                     LVTILCTPTKLNYLVLGFLYSEGIISNINDVVMMRVCDAESEVDVRLKDSGFQLPTKR
                     TLTSGCGGGATFTTGGQMVNSDLVVTPAVVLSQMKLLLEQEELYRLSGGVHTSVLSDG
                     RKLLVVTEDIGRHNTLDKIQGECLMRGITTKDRLILTTGRVSSEMLRKAARMQVPVLV
                     SRHTPTGTAMSMAKDLGIALIGQARGNRLSVYSHPERLGCPTQ"
     gene            complement(999..2543)
                     /locus_tag="PK125_11145"
     CDS             complement(999..2543)
                     /locus_tag="PK125_11145"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPB38700.1"
                     /translation="MKRNRIFLLWIIMLVFTVCSKAVAQDAKTYFEQGNDHFRSGNYL
                     EAIKSYDNALAIDPRDSRLFLLRGVSYAALGKYEDAIKDYDKSAELNPKYAATYGKRG
                     EAYAAMGKNSEAIKDFDMSIELNPKDASLYVRRGAAYAALGKYVEAIMNCDDAIKLSP
                     EYFEAYINRGIAHYMSGNYHEAIKNYDVAAKLNPRENTVNLLRGAPHAALGNYPEAIK
                     NYDRAISLNPTYVEAYSNRGLVYSMSGNYREAIKNYNKAIELNPKDIATYRRRADAHE
                     KTNSDDDAIKDYDKLIGFGLKDATVYLKRGSLFDKTGKFSDAINDFDEAIKLKPDYFE
                     AYYKKALVLYAMARYPESIATFDRAIEINPDFSVAYYNRGIAYAVTGNQVEAVRNYEK
                     ASSLNPKDPAIYVRQGDAYTILREYANAAQSYEKALNLDPKAPGVYYNRGLALAKLKD
                     HAEAIKSFSKAVELNPGFVNALYNRAVSFRELGREAEALNDYKAAARLGDHGSQYYLR
                     SKGIKW"
     gene            2759..3529
                     /locus_tag="PK125_11150"
     CDS             2759..3529
                     /locus_tag="PK125_11150"
                     /inference="COORDINATES: protein motif:HMM:NF014635.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF169 domain-containing protein"
                     /protein_id="HPB38701.1"
                     /translation="MDSAKKEKFIKLWEKYFGKADLPIIFFYTDNPVNVIKIKPPKAE
                     HRCVIADIARTRKGTSVALHVGAVGCFGGKKYLGFNQGFMPNFEYFLSCGIPGEMEGE
                     RYKKSPAIVKEVMKNMPSFKAPAEYIVFKRWDKIDAYDNPDVVIFFAPPDVLSGVFTL
                     ASFDEIEPEGVFAPFAAGCSTIVTHPYLEKSANRPRAVLGMFDVSARPSVPGNTLTIA
                     IPMNKFEKMIDNMEESFLITKSWAKVRRRITKAQKDKE"
     gene            complement(3806..4249)
                     /locus_tag="PK125_11155"
     CDS             complement(3806..4249)
                     /locus_tag="PK125_11155"
                     /inference="COORDINATES: protein motif:HMM:NF015444.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HPB38702.1"
                     /translation="MKYQVGEMGRIVVARFEDGDEIIQGLSDIAKKEDLRAAVFYLVG
                     GIKEGRIVVGPEKDVFPPTPVWKVLNESHESAGVGTIFWYGNEPRIHFHGVYGKHDMV
                     KTGCLRELARAFIVMEAVIIEIKGINAVRDLDPRSQMVLLKMIDS"
     gene            4529..4798
                     /locus_tag="PK125_11160"
     CDS             4529..4798
                     /locus_tag="PK125_11160"
                     /inference="COORDINATES: protein motif:HMM:NF012305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB38703.1"
                     /translation="MEGSKLYVGNLNYSSTVEEIKELFAGFGEVRDVRVIEGKGFGFV
                     DMGSQAEAEKAKKELHGSQFMGRTLTVDEARPKKDKPRQGGPRGY"
     gene            4897..5094
                     /gene="rpsU"
                     /locus_tag="PK125_11165"
     CDS             4897..5094
                     /gene="rpsU"
                     /locus_tag="PK125_11165"
                     /inference="COORDINATES: protein motif:HMM:TIGR00030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HPB38704.1"
                     /translation="MEIRVHDDNIEKAIKDLKRQLQKEGLFKEIKKRSFYEKPSVKDR
                     RKRREAQKRRMKAQRRKSFYD"
     gene            5139..5570
                     /locus_tag="PK125_11170"
     CDS             5139..5570
                     /locus_tag="PK125_11170"
                     /inference="COORDINATES: protein motif:HMM:NF012478.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HPB38705.1"
                     /translation="MTEAKVNDTVKVHYTGHLCDGTVFDSSFNREPLEFTIGQKMVIP
                     GFEKEVIGMTVGDTKTVSIASADAYGPYLEDLVGSIKRTQIPPNIDLQVGDVLQMRTP
                     DGGHMSVLVKALTDETVTLDANHPLAGKDLAFEINLLEITS"
     gene            5655..5867
                     /locus_tag="PK125_11175"
     CDS             5655..5867
                     /locus_tag="PK125_11175"
                     /inference="COORDINATES: protein motif:HMM:NF024820.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPB38706.1"
                     /translation="MEKNGIVSATLAEIYLEQGYLEKAIAIYDQLLAKEPENDSYRVR
                     LSSLKKTLKEKSRSPLFKRVLRNKNR"
     gene            5874..6947
                     /locus_tag="PK125_11180"
     CDS             5874..6947
                     /locus_tag="PK125_11180"
                     /inference="COORDINATES: protein motif:HMM:NF012766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HPB38707.1"
                     /translation="MRENRIEKVLSLMSSKKVDACLLKGMDNIFYLTGFRGSEGTLFI
                     TKGDIFLLTDFRYVTYAQEVTHGIRIIEVRDKRNVLGELCSQYKIERIGFDSIHTTFF
                     TFKKWSDAIENIEFIPLDATIEEIRKNKEPGEIDSIIKAIDIATQAFNDVFSRIHPGQ
                     TEKEVANNLDYAMRQLGAEGPSFPTIVASGPRAALPHAEPTDKRIRDGEMVIIDFGAH
                     VDGYCSDETCTLSIGDVNGKLQDIYTVVNNAKQLALEKARAGMPIKEMDMIVRGFIGS
                     AGYGEFFRHGTGHGVGIAVHEAPAINSTGDGLLEEGMVITIEPGIYIPNLGGVRLEDM
                     VLITSDAPRILTRVRKDELHIKA"
     gene            7034..7600
                     /gene="efp"
                     /locus_tag="PK125_11185"
     CDS             7034..7600
                     /gene="efp"
                     /locus_tag="PK125_11185"
                     /inference="COORDINATES: protein motif:HMM:NF001810.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HPB38708.1"
                     /translation="MMVSTSEFRKGLRILLEDEPFVIVDFQHVKPGKGGAFVRTRIKS
                     LISGNVLDRTFRSGDKVDLPELEESQMQFLYSEEDKFYFMDTNTYDQIFIDEKNLGDA
                     KNYIKEGLTIEVLFYKGNPIGVEIQNFVDLKIVKTEPGVRGDTATNVTKPALLESGYT
                     IQVPLFVEEGEVVKIDTRTGQYIERVSK"
     gene            7609..>8182
                     /gene="tmk"
                     /locus_tag="PK125_11190"
     CDS             7609..>8182
                     /gene="tmk"
                     /locus_tag="PK125_11190"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HPB38709.1"
                     /translation="MLITFEGIEGCGKSTQVDLLFDYLIKKGLRVIKTREPGGTAYGE
                     TLRDVALQKNLKVSPLSELITIMAIRAQHVEELIVPALKDRTIILCDRFVDASYAYQG
                     YGRGIDLGIIETLNRLVTRGISPTLTVLIDCTADMGLKRKEKNNRPLDRFEEEGLLFH
                     QKIRNAYLKLAKEDKKRFFVVDGTAEIDEVH"
BASE COUNT         2420 a         1731 c         1849 g         2182 t
ORIGIN      
        1 ggggtgtggt aagaaaggct aaagagaaag aggtagcctg aggggaaaga aaactctaac
       61 ggcctagcca caataagaat tggatcaaaa tggaagaggc aatgagtgca ccagacgtga
      121 agggtgtagc gactgatata ctgtgtagtc gttattccga aactggatgg acaaaggcaa
      181 aggttcacgc tcctcttgaa agagagttga cagtctatgt caacctccag catctggtca
      241 ccattctgtg cacccccacc aaactgaatt accttgtcct tggttttctt tattcagaag
      301 gaatcatatc gaatattaat gatgtggtaa tgatgagggt ctgcgatgct gagtctgaag
      361 ttgacgtaag actaaaagac tcggggttcc aattaccgac gaaacgaacg ctaacctctg
      421 gttgcggggg tggcgcgacc ttcacaactg gggggcagat ggttaattcg gaccttgttg
      481 taacaccggc tgtagttttg tcacagatga aactccttct tgagcaggaa gaactatatc
      541 gccttagtgg cggcgttcac acttcagttt tatctgatgg caggaaattg ctcgttgtaa
      601 ctgaggatat tggccgacac aataccctag acaagataca gggtgagtgt ctgatgaggg
      661 gaataacaac aaaagatcga ctgattctga ccactggtcg tgtctcctcc gagatgctcc
      721 gcaaagcagc aagaatgcag gtaccggttc ttgtttcccg tcatacaccc acaggcactg
      781 ccatgtcaat ggctaaagat ttgggtattg ccctgattgg tcaagcgcgg ggcaaccgcc
      841 tctccgtcta ctcacaccca gagcggcttg gctgtcccac acagtaactc gggcctcccg
      901 tgccaacccc tagaaaacaa ataaacaggc ctgatgcgcc catagcgcct gctcatgcat
      961 tattacggct tagagctatt ttttaggaag gccgagactt accatttaat tccttttgat
     1021 cttaaataat actgcgatcc atggtcacca agcctcgctg ccgccttata gtcattgaga
     1081 gcctctgcct cacggcccaa ttccctgaaa gaaactgccc ggttgtataa agcattcaca
     1141 aatccagggt ttagttcaac ggccttgctg aaactcttta ttgcctcagc atgatctttc
     1201 agctttgcta gggcaagacc acgattataa tagacgccag gcgcctttgg gtcaagatta
     1261 agggcctttt catagctctg tgctgcattc gcatactccc gcagaattgt atacgcatct
     1321 ccttggcgaa catagatagc cgggtccttt ggattgagac ttgaggcctt ttcatagttc
     1381 ctgacggcct caacctggtt acctgtcaca gcatatgcga tcccacggtt ataataagca
     1441 acagaaaaat caggatttat ctctattgcc ctgtcaaagg tggcaatgga ttcgggatag
     1501 cgagccatgg catacaaaac cagggctttt ttgtagtagg cttcaaaata atccggcttc
     1561 agcttgatag cttcatcaaa gtcgtttatg gcatctgaaa acttgcctgt cttgtcgaat
     1621 aaactgcctc gttttagata aacagtagca tctttcagac caaaccctat cagcttatca
     1681 taatctttta ttgcgtcatc gtcgctgttc gttttttcgt gtgcatctgc acgacggcga
     1741 tacgttgcaa tatctttagg gttaagttca atggctttgt tataattctt tatagcctcg
     1801 cggtaattgc ctgacatgga ataaaccaga ccacggttag agtaagcttc cacatatgtg
     1861 gggtttaacg atattgccct gtcgtaattc ttaatggctt cgggataatt acccagagct
     1921 gcatggggag ccccccgaag aagattgacg gtattttctc gcggattaag cttcgcagct
     1981 acatcatagt ttttgatggc ttcatggtaa tttcctgaca tataatgagc aataccacga
     2041 ttgatgtatg cttcaaaata ctccggactc agtttaatcg catcatcaca attcatgatt
     2101 gcctcaacat atttccccag agctgcgtat gcagccccac gccgaacata caatgaagca
     2161 tctttggggt taagctcgat tgacatgtca aaatctttga tagcctcgct gtttttgccc
     2221 attgccgcat aagcctctcc tcgcttacca tacgtggcag catattttgg gttaagctct
     2281 gcagatttgt cgtaatcttt tattgcatct tcatatttcc ctagagcagc atatgatacc
     2341 ccgcgaagga gaaagagcct gctatcccga ggatctattg caagggcatt atcatagctc
     2401 ttgatagctt caaggtagtt gccagagcgg aaatggtcat taccctgttc aaagtatgtt
     2461 tttgcgtctt gagctactgc tttggagcaa acagtaaaca ccaacatgat aatccataaa
     2521 agaaatatcc tgtttctctt cacttttcga tctcctcaga gttttgtagg ttaactgacc
     2581 ataggttata atcataccat aaaattatga atgtaaatga ttctttgaaa ggtttgcatt
     2641 tattgaatag aaaaataatg aaataacctg gtttttacat taaaatgttg gtgtcacgca
     2701 ggacagaaca ggtcatacgc tttttttatc aaaatattgt gatcatggag gacaggtaat
     2761 ggattcagca aaaaaagaaa agtttataaa actgtgggaa aaatattttg gcaaagcaga
     2821 cttaccgatc attttcttct acactgacaa tccggttaat gtaataaaga taaaaccgcc
     2881 aaaggcagag cacaggtgcg ttattgcaga catagcacga acaagaaaag gaacgtctgt
     2941 tgccttgcat gttggtgcag ttggctgttt tggcggtaaa aagtatctgg gattcaacca
     3001 aggttttatg ccaaacttcg agtactttct ctcgtgtggg atacccgggg aaatggaagg
     3061 agaacgttat aaaaagtcac cggccattgt aaaggaagtc atgaaaaata tgccaagctt
     3121 caaggcgccg gcagaataca ttgtttttaa gagatgggat aagatcgacg cgtatgataa
     3181 tcctgatgta gtaatcttct ttgcacctcc agacgtactt tcaggcgtct ttaccctagc
     3241 aagctttgac gaaatcgaac cagagggggt atttgcacct tttgctgccg gttgttcaac
     3301 catcgtaacc catccgtatc ttgaaaaatc agcaaaccgc cctcgggcag tgcttggtat
     3361 gtttgatgtt tctgcacggc cctctgtccc tggcaacaca ctcacaattg caatccctat
     3421 gaataagttt gaaaagatga tcgacaatat ggaggagagt tttttaataa ccaagagttg
     3481 ggcaaaggta cggcggcgga taactaaagc gcagaaagat aaagaataaa tagccagcat
     3541 tgacacatgc gctggaatct gcccagtaaa taccatgagt ttgaagggta aattcgaaca
     3601 gctttacaat ggacgggacg actgccttcc gaagcctcag cgcagataga cacatgcccc
     3661 aacaatgtga attggttggc gggaaaaaac aaaagctgct gtggtcagcc agggcatagt
     3721 cagattgcta acgtcagtgc catggtgata aattaattcg tacctcaaca ctgtaagggg
     3781 ctcttttaat aaaaactggg ccctttcaac tgtcaatcat cttgagcaat accatctgtg
     3841 agcggggatc gaggtcgcgc actgcattga tgcctttgat ttcaataatg acagcctcca
     3901 tgacaatgaa tgcccttgca agttcacgga ggcatcctgt ctttaccata tcatgttttc
     3961 catatactcc atgaaaatga attcttggct cattcccgta ccaaaatatc gtgcccacac
     4021 cggcagattc atggctttca ttcaatactt tccatactgg ggtcgggggg aacacatctt
     4081 tttccggtcc caccacaatc ctcccctctt ttattccccc taccagataa aacactgcgg
     4141 ctcgaaggtc ctctttcttt gcaatatcag acaggccctg tatgatttcg tctccatcct
     4201 caaaccttgc gaccacaatt ctgcccattt caccgacttg atatttcatg gttatcccct
     4261 cacaagcttc taattcaatg gatattttat tacatttgtg gtcacttcgc tacaaaccgc
     4321 tttctctaag cgaaataaaa agcttccaat tcttgcccaa ttgttatatt atacattcta
     4381 ttgttggtta cccttaacag tatcagggta taggctgatt tggtagtggg attttattcc
     4441 tgtgcactgt agtttcatat ggagcggaac aggatccaca ttcacccctc cacaagaaac
     4501 gatttaattc aacaaagtgg agggttgaat ggaaggcagt aaactgtatg tgggtaacct
     4561 gaattattcc tcgaccgtgg aagaaattaa ggaactgttt gccggttttg gcgaggttcg
     4621 tgatgtgaga gtaatcgagg gaaaaggatt tggctttgtt gatatgggtt cacaagccga
     4681 agcagaaaaa gcaaaaaagg agttgcatgg ttcacaattt atgggccgaa ccctcaccgt
     4741 tgatgaagca cgccctaaaa aggataaacc gagacagggc ggcccacgag gatattaaaa
     4801 aacttcttta ataattgaca gattcccctt tatgggcaca ccgaacgatc acgtatttca
     4861 caaatcaata caggggaaag aaagggaaag aaaacattgg aaataagagt acatgacgac
     4921 aacattgaaa aagcaatcaa ggatctgaag cgtcagctcc aaaaagaagg tctctttaaa
     4981 gagattaaga agagaagctt ctatgaaaaa ccttctgtaa aggaccgaag aaaaaggaga
     5041 gaggcgcaaa agagaaggat gaaggctcaa cgtcgtaaaa gcttttatga ttagaaatat
     5101 gccagaagcc tgcaattcca aaacattaag aggaaattat gacagaggct aaagtcaatg
     5161 acactgtaaa agttcattat accggtcact tgtgtgacgg cacagtgttc gattcatcct
     5221 tcaacagaga accgctcgag tttactattg gacagaaaat ggtgattcct ggctttgaga
     5281 aagaggtcat tggaatgact gtgggagata caaagactgt ctcaatagca tctgcggatg
     5341 cctacggtcc ttatttggaa gatcttgtcg gttctataaa aaggacccag atacctccaa
     5401 acattgacct gcaagtagga gacgttttgc agatgcgcac acctgatggt ggacacatgt
     5461 cagtcttagt taaagcattg actgatgaaa cagtcacttt ggatgcaaac catccccttg
     5521 caggtaaaga tttagcgttt gaaataaacc ttctcgagat aacatcgtag ttctctgtaa
     5581 agattactga ttgatctttt gttttggtag ttttaacttg atttttacag ggagatttct
     5641 ttaactatcc aatcatggaa aagaatggaa ttgtttctgc aacgctggca gagatatatt
     5701 tggagcaagg ctatcttgaa aaagcaatag caatctatga ccagctcctg gcaaaggagc
     5761 cggaaaatga cagctataga gtgaggcttt cttctctcaa aaaaaccctc aaggaaaaaa
     5821 gcagatcccc cctctttaaa agggttcttc gtaacaaaaa ccggtgacag tacatgagag
     5881 aaaacaggat tgaaaaagtt ctatccctca tgagttcaaa gaaggtagat gcgtgcctcc
     5941 ttaagggcat ggacaacatc ttctatctca ccggctttcg tggcagcgaa ggaacacttt
     6001 ttatcacaaa aggcgatatt ttcctgttaa cagatttcag gtatgttact tatgcgcaag
     6061 aggttacaca tggtattaga ataatcgaag ttcgtgacaa gcgaaacgtc cttggagaac
     6121 tatgcagcca gtataaaatc gagcgaatcg gttttgatag cattcacaca acgtttttca
     6181 cattcaagaa atggtccgat gcaattgaaa acatcgaatt tattcctctc gatgctacaa
     6241 ttgaagaaat aagaaaaaac aaagagcctg gtgagattga cagtattatc aaagcaatag
     6301 acattgcgac ccaagcattc aatgatgttt ttagtaggat tcatcccgga cagacagaaa
     6361 aggaggttgc aaacaatctg gactacgcta tgcgccaact tggcgcagaa ggcccttctt
     6421 tccctacaat agtagcctca ggaccaaggg cagcccttcc ccatgctgaa ccaaccgata
     6481 aaaggattcg tgatggtgaa atggtcatca tagatttcgg cgcccacgtc gatggttatt
     6541 gtagcgatga gacctgcaca ctttcaattg gtgatgttaa cggaaaattg caggatattt
     6601 acacagtcgt aaacaatgcg aaacaacttg cccttgaaaa agcacgggct ggcatgccca
     6661 tcaaagaaat ggatatgatt gtaagaggtt ttattggttc cgcaggctat ggagaatttt
     6721 tccggcacgg cacaggccat ggtgttggaa tcgccgttca tgaggctcca gctatcaaca
     6781 gcactggcga tggcctcctg gaagagggaa tggtcataac cattgagccc ggaatctata
     6841 ttccaaattt gggcggcgtt cgtttggaag atatggtact cattacttct gacgcccccc
     6901 ggatactcac gcgagttcgt aaggatgagc tccatataaa agcataggca cgcatcattg
     6961 tttgccacag gaatttttac tgatttacgc tatgttctga atgcttagcg ctacatcact
     7021 cggaggtgtt aatatgatgg tatccacgtc agagtttcga aagggcttaa ggattcttct
     7081 ggaagatgaa ccctttgtaa ttgtcgattt ccaacatgtc aaacctggca aaggaggcgc
     7141 ctttgtaagg acaaggataa aaagtcttat atcaggaaac gtgttggata gaactttcag
     7201 gtcaggggat aaggttgacc tccctgaact cgaagagtcc cagatgcagt ttctatacag
     7261 cgaagaggac aaattttact ttatggatac caacacctat gatcagatat ttatcgacga
     7321 aaagaatctg ggagatgcaa agaactatat taaggaaggt cttacaatcg aggtgctgtt
     7381 ctacaaagga aaccctatcg gtgttgagat tcagaacttt gtggatttaa agattgttaa
     7441 aacagagcct ggtgttcgcg gggacacagc aacaaatgtg acaaaacctg cccttctcga
     7501 gtcagggtat acgatacagg ttcctttatt cgtggaagaa ggagaagttg ttaagattga
     7561 caccagaaca ggccagtata tagagcgggt ttcaaagtag gattgtccat gcttataacc
     7621 ttcgaaggca tagaaggatg tggtaaatca acccaggtcg atcttctctt tgattatttg
     7681 atcaagaagg gcctcagggt gatcaagact cgtgaaccag gtggtacagc ctatggtgag
     7741 acgctcaggg atgttgccct tcaaaaaaat ttgaaagtgt ctcccttatc ggagcttatc
     7801 acaatcatgg cgatcagggc acaacacgtt gaagaactca tagtacccgc cctaaaggat
     7861 agaacaataa ttctctgcga caggtttgtt gatgcgtcct atgcatacca ggggtatggc
     7921 cggggcatag accttgggat cattgaaacc ctcaatcgac tcgtgaccag aggtattagt
     7981 cctaccctta ccgtgctcat cgattgcacg gctgacatgg ggttaaagag aaaggaaaag
     8041 aacaacaggc cactcgacag attcgaggaa gagggtctct tgtttcacca gaagatacgg
     8101 aacgcttacc tgaaacttgc aaaagaagac aaaaaaaggt tttttgttgt agatggaacg
     8161 gcagagattg atgaagtaca ca
//
LOCUS       DAOOMC010000122         8056 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdus sp. isolate AMR_MDS_2685
            NODE_10499_length_8056_cov_6.082240, whole genome shotgun sequence.
ACCESSION   DAOOMC010000122 DAOOMC010000000
VERSION     DAOOMC010000122.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864760
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdus sp. (wastewater metagenome)
  ORGANISM  Syntrophorhabdus sp.
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae; Syntrophorhabdus.
REFERENCE   1  (bases 1 to 8056)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8056)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,729
            Genes (coding)                    :: 2,708
            CDSs (with protein)               :: 2,708
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 7 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8056
                     /organism="Syntrophorhabdus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10499_length_8056_cov_6.082240"
                     /isolate="AMR_MDS_2685"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2699756"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(70..345)
                     /locus_tag="PK125_11195"
     CDS             complement(70..345)
                     /locus_tag="PK125_11195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB38710.1"
                     /translation="MKGNHKETDVIGFFKNRQIYIELRPRCPKCKKEFMLDLKKFLPG
                     KAHGCHACGTIARFDAQLAERVQKLIHDLELSLREVHESFASQEAHE"
     gene            complement(365..1168)
                     /locus_tag="PK125_11200"
     CDS             complement(365..1168)
                     /locus_tag="PK125_11200"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HPB38711.1"
                     /translation="MMRSLYHRSKNASKFSAPLVPQQELDLPGILFHDLRSSLTSLFA
                     VIHMQDSGCHESTDYPVKAKLAVFINKIGNFSSMLEESLQMVCALDEDNRLELHLVDL
                     RFHVVEPVISVFISDVKTKRISMQTRLGPVTGFLNNYRINKTILQMIFHILLSDAVNN
                     CEFSGINDIDIKPDSPFFLLKIANTGRPITEELYTKLFLKPLPPKDRKETNPDGMGLG
                     LHLAKQIIQTHGRDIWHEPDTGRTAFILAIPKEISDTHFTTHTKDTLFS"
     gene            complement(1381..2265)
                     /locus_tag="PK125_11205"
     CDS             complement(1381..2265)
                     /locus_tag="PK125_11205"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HPB38712.1"
                     /translation="MNKYDIVFIGHVTVDEIVATEGASSGTGGGAPLFGAIAASVTGK
                     KIAVITRIAKEDKYLVEALEKNGIDVYLEYAQNTTHMRVVHPSANIDERQMYQTKDAG
                     FFKIDEIPSFETRLIHLGALTDREFTLEYMKKLRKKGFRLSVDMQGFVRQVDKSTRII
                     RFGDVPEKKEIMGLADFVKLDLVEAEILTGTDNLSKAAGICEAWGSQETLITRSEGVL
                     VRKDGKDYFEHYSNRSNSGRTGRGDTTFGAYLSKRLDNSIEESIKFAASLASIKMEVP
                     GPFAGTLADVVKRMNRVL"
     gene            complement(2345..3211)
                     /locus_tag="PK125_11210"
     CDS             complement(2345..3211)
                     /locus_tag="PK125_11210"
                     /inference="COORDINATES: protein motif:HMM:NF012760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016742 - hydroxymethyl-, formyl- and
                     related transferase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyltransferase family protein"
                     /protein_id="HPB38713.1"
                     /translation="MTGELVFDPSSRTMIYACGVSGSGTNYDRIYEGDPQKKHLVFSN
                     VPGCKGMQKAWSYGVQAVSLDSSIYFREMWGLERAPRHGIERNSFDMAIMTLVEQTMG
                     SQPDLICLAGYDLWIGDWMVSRYYPRILNVHPADATKYVGLGWIPTAKAILAGEKSVK
                     STVFFVDQSDDGGPILIQSASLPLSQWDNQLRDVRGFAEKTGSETLKDFREATEREGN
                     NLYESLKMVSSEIQEILKTEGDWKIYPFAVHELIAKGRVALDGRTVYIDGMKMPPQGW
                     QVDTYGFAHNIG"
     gene            complement(3289..4788)
                     /locus_tag="PK125_11215"
     CDS             complement(3289..4788)
                     /locus_tag="PK125_11215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB38714.1"
                     /translation="MMTIACLVAAAFAGALAMTPFPVFGQSRYEGSVWKGTITFTYRE
                     AGGDKSTSSRNQIDREEQWSLSNRFTLILNVCGNSLEQAYVKNAFVDYTHTWKNKEIW
                     PKAVCLDTKEVKKPGFRTSDEGSMSAVIHPNLVGSKADAALAVSLQPSGGIRYTISAG
                     LGLDAPMALLVGSSQSKQYDPCENTTVSHTLEYTAGLDNARQKCSNDGKTCHSLVPAG
                     ETAFPMTFSHAGTSTGPCIAGKMTIPVKKMGAMILSSLASQMEEMARQMPEEIRSQMM
                     GEIKKIEKESSKEISQKSGSTDTKGKTTAALDVVWSLRKNDPCDAVLDQLRQELSMVQ
                     AYADTSLLSKARREGWTGEQYDNAVFENGKAQYASGWWKNRSSRGKQGNPGKPSDGDH
                     NKHIDMALNNERCEIVGQEEKQKALRQDCMPQIIYESILEHEKTHAKQCMSKDTAAEF
                     ASRTPDSFRKFEQAAYCVGAKKLMKWAEGVCKGSDVKPLREMYEQFCPK"
     gene            complement(4842..5669)
                     /locus_tag="PK125_11220"
     CDS             complement(4842..5669)
                     /locus_tag="PK125_11220"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HPB38715.1"
                     /translation="MKVKTYRFCCFIAAFALCLTPCASFTQDAPGCRDHQLFTRMPNF
                     YIQQCETKEFDSHTFYLSEDKEIVVEGKKTYVSHVIREGAKAPSDLQILRNFENAVSR
                     IGGKVEYKKKYDAYLSIKKGGKETWVQVTSWNDGEGYDLTIVEKKEMEQEITANAMFD
                     ALNRDGFIALYINFDTNKATIKPDSKPIVEQIVVLLKNNPGLKVSIEGHTDNTGTPVR
                     NKTLSKERAQSVVSALVSAGIDKQRLSAIGWGQEKPIADNRTDEGKAKNRRVEIVKK"
     gene            complement(5682..6125)
                     /locus_tag="PK125_11225"
     CDS             complement(5682..6125)
                     /locus_tag="PK125_11225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB38716.1"
                     /translation="MNRLKGIVLAGVILFMGVIVVSAADFKVYPGAKVDEKLTKEAND
                     FAARSVAGSKMAAPKATIYTTGDAYEKVYSFYKSVGKEYQMPGTSGRKNTLPSGKELK
                     SSFFIFDGAIDLMSSKLWIKIQRPYIDTEMREGPDMTYIIVSTKN"
     gene            complement(6361..>8056)
                     /locus_tag="PK125_11230"
     CDS             complement(6361..>8056)
                     /locus_tag="PK125_11230"
                     /inference="COORDINATES: protein motif:HMM:NF012348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamate-ammonia ligase
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glutamine synthetase III"
                     /protein_id="HPB38717.1"
                     /translation="KTDESGNATLTIPTAFYSYYGEALDKKTPLLRSMKAVSKQALRV
                     LRALGNTTSTRVTSTVGPEQEYFLVDKKFYLERLDLMLAGRTIFGAPAPKGQELEDQY
                     FGTIKDRVSHYMHDLNIELWKMGITSKTQHNEVAPAQYEMAPVFSTTNIGTDQNQLVM
                     ETMQKVAFRHELVCLLHEKPYAGINGSGKHNNWSLLTDDGINLLDPGHTPSDNVQFLL
                     FLSALLRAVDLHADILRATCATAGNDHRLGANEAPPAIISIFMGEELTDILKKISNGE
                     KISTKDTQFVAVGVDTLPIIPKDNTDRNRTSPFAFTGDKFEFRMVGSAQSTAGPNVAL
                     NTIAAEALDEIATRLEKAKDKDNEAAAIIKEIYSKHNRVIFNGNNYSEEWVREAQKRG
                     LPNVRNAVDALKAFITDKSLKLFEKYEIFSHKELHSRYDIYVDIYSKQINIEALTAID
                     MVKKQYLPVCLEYATFLADSIGSFSAVSVSAPVQEDLLKKLNTHVASSYKNLAKLESA
                     LAKTQTIESIVEKAEAYRDKVITAMGALRKDIDALEMIVPRDMWPVPSYTDLLFKL"
BASE COUNT         1894 a         1919 c         1799 g         2444 t
ORIGIN      
        1 gtatagaagg tgcctttatg ttttttgtca tggaaggctt tgtatggaat acttgttttt
       61 tcatcaccat tattcatgcg cttcctgtga tgcaaagctt tcatgaacct ctcgaagaga
      121 taattccagg tcatgtataa gtttttgcac cctttcagca agctgtgcat caaaccgtgc
      181 aatggtgccg catgcatggc agccatgtgc ctttcctgga agaaatttct ttaaatcgag
      241 cataaattct tttttacatt tggggcaccg gggccttaat tctatatata tctgcctgtt
      301 cttgaagaag ccaatcacat cagtctcctt atggtttccc ttcatcccct tctatatctg
      361 gaaatcagga aaaaagcgtg tctttggtat gtgttgtaaa gtgagtatcg cttatctcct
      421 tcggaattgc caaaatgaaa gctgttctcc ctgtgtccgg ttcatgccat atgtcccggc
      481 catgagtttg gattatttgt tttgcaaggt gaagaccgag gcccatccca tctggatttg
      541 tctcttttcg atctttcggg gggagaggtt tcaaaaagag ctttgtgtat aattcctctg
      601 tgatgggcct cccagtgtta gcaattttca aaagaaaaaa agggctatca ggtttaatgt
      661 caatatcatt gatgccactg aattcgcagt tattcacggc atcgcttaag aggatgtgaa
      721 agatcatttg aagaatagtc ttgttaattc tgtagttatt taaaaaacct gttacaggac
      781 ccagtctggt ttgcatactg atcctcttcg tttttacgtc tgaaataaat actgaaataa
      841 cgggttcaac tacatggaat ctcaaatcaa caagatggag ctctaaccgg ttatcttcat
      901 caagagcaca caccatttga agagattctt ccaacatgga agaaaaattc ccaatcttat
      961 tgatgaatac tgccaatttt gcctttacag gataatcagt gctctcatgg cagccactgt
     1021 cctgcatatg gataaccgcg aatagggagg tgagagagct tcgcaaatca tggaaaagaa
     1081 ttcctggcag atcgagctct tgttgtggta ctaatggtgc agaaaacttt gaagcattct
     1141 ttgacctgtg atagagcgac ctcatcatga tcccttcgtg gtatattaca aggatttcta
     1201 atgagcaaaa ccagtgccag tagaatattc aaatatatgt atgtgaaatg cttgacattt
     1261 acttgcgagg cttgtttcgt caggaaagat ccctgaatgg atttaccttc accctgcaga
     1321 agaggctggt ggttatttcc tccatcgtcc tgaagacgaa gattggtctt acagggagca
     1381 ttaaagtacc ctgttcattc ttttgacaac atctgcaagg gttcccgcaa acggccccgg
     1441 tacttccatt ttgattgatg caagggatgc agcaaatttt attgattctt caatggaatt
     1501 gtccagccgt tttgaaaggt aggcgccgaa tgtggtgtcg ccgcgtcctg ttcttccaga
     1561 gttactcctg ttggagtaat gttcgaagta atcctttcca tcctttcgta caagaactcc
     1621 ttcagagcgg gtgatcagcg tttcctggct tccccaggcc tcacaaatgc cggcagcttt
     1681 tgatagattg tctgttcctg taaggatttc tgcttcaact aaatcgagtt ttacgaaatc
     1741 tgccaggccc ataatttctt ttttttcagg gacatcgcca aatctaatga ttcgcgtcga
     1801 tttatcaacc tgccggacaa aaccctgcat atcaacagat aaacgaaatc cttttttcct
     1861 gagttttttc atatattcca gggtaaattc ccggtcggta agtgccccca gatggatcag
     1921 acgcgtctca aaagagggta tctcgtctat cttgaagaac ccggcatctt ttgtttggta
     1981 catctgtctt tcatcaatat tggcagatgg atggacaacg cgcatgtgtg ttgtgttttg
     2041 agcatattca agataaacat caatgccgtt tttttctaat gcttcaacga ggtatttatc
     2101 ttccttggca atccgtgtta ttactgcaat tttcttccct gttacagatg cagcaatagc
     2161 cccgaagagc ggtgcaccac cccctgtacc actggaagcc ccttctgtcg caacaatttc
     2221 atcaaccgtt acatgtccta taaagacaat atcatacttg ttcatgaaaa attattctaa
     2281 atcctgaacc ccatgatctc acaattccgt agccttttat tttctcagtt tttttattgt
     2341 gcttttaccc tatattgtga gcaaatccat acgtatccac ttgccagccc tggggaggca
     2401 tcttcatacc atcgatatag actgttctac catcaagggc aactcttcct tttgcgatga
     2461 gctcatggac agcaaagggg tagattttcc agtcaccttc tgttttgaga atctcctgaa
     2521 tctcgcttga taccattttc agcgattcat aaaggttgtt accctctcgt tctgttgcct
     2581 ctctgaagtc tttcaatgtt tctgagccgg ttttttcggc aaatccccgc acatctctca
     2641 actgattgtc ccattgtgag agtggcaacg acgctgactg aataaggatg gggcctccgt
     2701 cgtcactctg atcaacgaag aatacagtcg attttacact tttctctccg gcaagtatgg
     2761 ctttggcagt tggtatccac ccaaggccaa cgtatttcgt agcatcggcg ggatgaacgt
     2821 tgagaattct tggataatac ctgcttacca tccagtcacc gatccagaga tcatatcctg
     2881 ccaggcatat gaggtccggc tgactgccca tggtctgttc tacaagtgtc atgattgcca
     2941 tatcgaagct gtttctctct atgccatggc gtggcgccct ttcaaggccc cacatttccc
     3001 tgaaatatat ggaagaatcc aaagatacag cctggacgcc ataactccat gccttttgca
     3061 ttcccttgca accaggcaca ttactgaata cgagatgttt tttttgaggg tctccttcat
     3121 aaattctatc gtagttggtc ccggaaccgg atacaccgca ggcatagatc attgtccgtg
     3181 aagaaggatc aaaaacgagt tccccagtca taattcacat ctatccttgt tctctgccgc
     3241 agcagcgcag cagacggtat ggactctcat tccttttcat acttccccct attttggaca
     3301 aaactgctcg tacatttccc tgaggggctt cacgtcggat cccttgcaaa ccccttctgc
     3361 ccacttcata agtttttttg cacccacgca gtacgcggcc tgctcgaact tgcggaaaga
     3421 atccggcgtc cgtgacgcaa actcagcagc ggtatctttg gacatgcact gttttgcgtg
     3481 ggttttttca tgctcaagga ttgattcgta gattatttgt ggcatgcaat cttgcctgag
     3541 ggctttctgt ttctcctcct ggcccacgat ctcgcagcgt tcgttattga gggccatgtc
     3601 gatatgtttg ttgtggtctc catccgaggg ctttccgggg ttaccttgct tccctcggga
     3661 ggatctgttt ttccaccatc ctgatgcata ctgtgctttt ccgttctcaa aaactgcatt
     3721 atcgtattgc tctcctgtcc agccttccct ccgggccttg ctcaagaggc ttgtgtctgc
     3781 gtaagcctga accatggaca gttcctgacg taactgatcg aggacagcat cgcaggggtc
     3841 gttctttcgc aaactccata ccacatccag tgcggctgtg gtcttgccct tcgtgtccgt
     3901 cgaaccgctc ttctgagata tctcctttga tgattctttt tctatcttct tgatttcccc
     3961 catcatctgg ctgcggattt cctcgggcat ctggcgagcc atttcctcca tttgggaggc
     4021 aaggctcgaa aggatcatgg cgcccatctt cttaacgggt atagtcattt tacccgcgat
     4081 acagggcccg gttgatgtgc cagcgtggct gaatgtcata ggaaaggcag tctcccctgc
     4141 cggcaccagt gaatggcacg ttttcccatc gttagaacac ttctggcgag cgttgtctag
     4201 tccagccgtg tattccaggg tatgtgatac tgtcgtgttt tcgcacggat catactgctt
     4261 tgactgagag ctgccgacga gcagggccat cggtgcatca agaccaagac ctgcggaaat
     4321 agtgtagcga attcctcctg atggctggag ggaaacagca agcgctgcgt ctgcttttga
     4381 acccaccaga ttgggatgga tcacggctga catggaaccc tcgtccgatg ttctgaatcc
     4441 aggttttttc acttcttttg tgtcgaggca tactgctttt ggccagattt ccttgttctt
     4501 ccaggtatgc gtgtagtcta caaaggcatt tttcacatat gcttgttcca gagaatttcc
     4561 gcatacgttc aggatcaggg taaacctgtt agaaaggctc cattgttcct ctcggtcgat
     4621 ctggtttcgg gaactcgtgc ttttatcccc cccggcttca cggtacgtaa acgtgatggt
     4681 acccttccaa acggaccctt catatcggct ctggccgaag acgggaaagg gtgtcatcgc
     4741 cagggcccca gcgaaggcag ccgccacaag gcatgcgatg gtcatcatga atgtgtctcc
     4801 tcagacagca atggtcaaag atccacagga aaacgaggat cctatttctt tacgatctca
     4861 acccggcggt tctttgcctt gccctcatcg gtcctgttat cggctatagg tttttcctgg
     4921 ccccagccga ttgccgacag acgttgcttg tcgatgcccg cggaaaccag ggcgcttacg
     4981 acagactggg cgcgctcctt cgacagagtc ttgtttcgaa caggtgttcc cgtattgtct
     5041 gtatgccctt caatgcttac tttgaggccg ggattgtttt ttagaagaac cacaatctgc
     5101 tctacaatag gttttgaatc gggctttata gtggccttgt tggtatcgaa gtttatgtac
     5161 agggcaataa aaccgtccct gttcaaagcg tcaaacatag catttgccgt aatctcctgc
     5221 tccatctcct tcttttcgac aatggtaaga tcgtagcctt cgccgtcatt ccatgacgtt
     5281 acttgtaccc acgtttcttt gccgcctttt ttgatgctga gatatgcatc atactttttc
     5341 ttgtactcta ctttgccgcc gatacggctg acagcattct cgaaattcct gagaatctga
     5401 agatcagagg gagccttcgc gccttcccgg atgacatgag atacatacgt ctttttccct
     5461 tctacaacta tctctttatc ctcagagaga taaaaggtgt ggctgtcaaa ttccttggtt
     5521 tcacactgct ggatgtaaaa gttcggcatc cgcgtgaaaa gctgatggtc cctgcaccca
     5581 ggagcgtcct gggtgaaaga agcgcacggg gtaagacaca atgcgaatgc tgcaatgaag
     5641 caacaaaatc tgtaggtttt gaccttcatg gacacctcca atcagttttt tgtcgacacg
     5701 atgatgtacg tcatgtcggg tccctctctc atttccgtat caatgtaggg tcgttggatt
     5761 tttatccaga gctttgaact catgagatct atagctccgt caaaaatgaa aaaggaggat
     5821 ttaagctcct tacccgaagg aagcgtgttc tttcttcccg aagtacctgg catctggtat
     5881 tccttcccca cgctcttgta aaatgaatag actttctcgt aggcatctcc cgttgtgtag
     5941 atggtcgctt tgggggcggc cattttagac ccagctactg acctggcggc aaaatcgttc
     6001 gcttcctttg tcagtttttc atcgactttg gcccctggat aaaccttgaa gtctgccgcc
     6061 gagacaacga taacgcccat aaaaagtatt actcccgcta gaactatacc tttcaatcga
     6121 ttcattccaa acctccctag ttttatacaa taagtttctt catggatcgt ccataccaaa
     6181 gggtcttcgc gagttgcata tttgcaagta tactgtaatt atatcagatg aagattgcga
     6241 atcaagagat ttgataatct tttgtccagg ggtagacatt aatcgtgact acactgcagg
     6301 gaagagaccg gactcagccc tttaaagccg gtctctcgtt ttgtagcgag ggtttgccgg
     6361 ttacagctta aacaataaat cggtgtaact cggtacaggc cacatatctc tgggaacaat
     6421 catttccagg gcatcgatat cctttcggag agcacccatg gctgtgatta ctttgtcgcg
     6481 ataagcctca gccttttcga caatgctctc aattgtctgg gttttggcaa gggccgattc
     6541 taattttgca aggtttttat aggatgaggc cacatgagta ttgagctttt tcaggaggtc
     6601 ttcctggaca ggagcagaca cagaaacagc actgaagctg ccaattgagt ctgcaaggaa
     6661 tgtggcatat tcgaggcaga caggtaaata ttgtttcttg accatgtcga ttgctgtcaa
     6721 agcttcgatg ttgatctgtt ttgagtatat gtcaacgtag atgtcataac gtgaatgcag
     6781 ttctttatgc gaaaaaattt catatttttc gaaaagtttc agcgacttgt cagttatgaa
     6841 tgcttttaat gcatccacag cgtttctcac attggggaga ccccgcttct gtgcctccct
     6901 aacccattct tccgaatagt tgtttccatt aaaaattacc ctgttgtgtt tactgtatat
     6961 ttctttgatg attgctgcag cctcattgtc cttgtctttt gctttttcca gcctggtcgc
     7021 aatttcatca agcgcctctg ctgcgattgt gttcagggcc acattaggtc ctgcggttga
     7081 ctgggcagag ccaaccattc tgaactcgaa cttgtcaccc gtgaaagcaa agggggaggt
     7141 tctattacgg tctgtgttgt cttttggtat tatgggaagg gtgtcaacac caactgcaac
     7201 aaactgtgtg tcttttgttg aaatcttttc tccatttgaa atctttttga ggatatctgt
     7261 taattcctca cccataaaaa tggagattat tgcaggaggc gcttcgtttg cccccaggcg
     7321 gtgatcattg ccggcggtgg cgcatgtggc tctcaggata tcagcatgaa gatcaacagc
     7381 cctgagtaat gcgctcagga acaaaagaaa ctggacattg tcactgggtg tgtgaccagg
     7441 atcaagcagg ttaattccgt cgtcagttaa gagtgaccag ttattatgtt tacccgagcc
     7501 gtttatgcct gcgtagggct tctcgtgaag cagacatacc agttcatggc ggaaagcgac
     7561 tttttgcatg gtttccatga caagttgatt ctggtctgta ccaatgttcg ttgttgaaaa
     7621 aacaggggcc atctcatact gggcaggtgc aacctcgttg tgctgggttt tggaggtaat
     7681 gcccattttc cagagctcta tatttaaatc atgcatgtaa tgggagaccc tgtcttttat
     7741 ggtgccaaaa tactggtctt ccagctcttg acctttggga gccggtgcac caaaaatggt
     7801 ccttccagca agcatcaggt caagtctttc cagataaaat ttcttgtcaa caaggaaata
     7861 ctcctgttcc ggccctacag tagaagtcac tcttgtggag gtagtatttc cgagtgcgcg
     7921 caagaccctc agcgcttgct ttgaaacagc cttcatggag cgaagcaggg gggtcttttt
     7981 gtccagcgcc tccccgtaat atgaataaaa agctgttggg atggtaaggg tggcgttacc
     8041 gctttcgtca gtcttg
//
LOCUS       DAOOMC010000123         7941 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdus sp. isolate AMR_MDS_2685
            NODE_10705_length_7941_cov_6.243850, whole genome shotgun sequence.
ACCESSION   DAOOMC010000123 DAOOMC010000000
VERSION     DAOOMC010000123.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864760
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdus sp. (wastewater metagenome)
  ORGANISM  Syntrophorhabdus sp.
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae; Syntrophorhabdus.
REFERENCE   1  (bases 1 to 7941)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7941)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,729
            Genes (coding)                    :: 2,708
            CDSs (with protein)               :: 2,708
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 7 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7941
                     /organism="Syntrophorhabdus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10705_length_7941_cov_6.243850"
                     /isolate="AMR_MDS_2685"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2699756"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..361
                     /locus_tag="PK125_11235"
     CDS             <1..361
                     /locus_tag="PK125_11235"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HPB38718.1"
                     /translation="EIPVFGDGHILRDFLYIKDLVSCMLMTAITDEAYGKVFNVGTAI
                     PVSFLDLAKKIVEIAGTGKVAHTEFTQERKEVEPGDYYADISRIKKIVKWFPKVSLEE
                     GIKQTIDFYRKNKKEYW"
     gene            366..1088
                     /locus_tag="PK125_11240"
     CDS             366..1088
                     /locus_tag="PK125_11240"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HPB38719.1"
                     /translation="MDEQSIRPLARNGVHEKVIAYLTDKPRGRILDVPTGQGALAKKL
                     HEVGFTVSCCDIEPAQFLVKGLTVEKGDLNGRLPYGDEEFDYVCFLEAIEHMENPYNA
                     VREVTRVLKRGGTLALSTPNYLNIERRMKFLITGFFTKPVSHERFLNACKGRTYGLHL
                     SPLGYTLIRFALEASGLKIVHLTYDMKKPKIIFFKPLTLFIRLYTRFWSKDKQKEYWF
                     DETTSKVILEGGNTLIIFAEKV"
     gene            1226..2335
                     /locus_tag="PK125_11245"
     CDS             1226..2335
                     /locus_tag="PK125_11245"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HPB38720.1"
                     /translation="MNVKMFDLARDHKEIRSELLGITERILTNGEFILSKEVEAFEDA
                     FAQYIGAKYAVGVGSGTDAIKIAGLAYGLEKGDKIVTTPNTYIATVMSLSLHGIIPVF
                     CDVETSTYNMDPERLEDILRKEKGIKLCIPVHLYGYPSSLDEIAAICRQNGVGILEDA
                     CQAHGSLYKGKKVGGFGDVSAFSFYPTKNLGCYGDGGMLVTNSDSIYKNALMLRNHGQ
                     HERHVHVVEGYNSRLDELQAAILRYKLTKLDIWNEKRRKIASWYKKKLKDVPVALPVE
                     EPWGYHVYHLFVIRAEKRDDLMKYLFHKGITTLIHYPIPIHLQKTYQTLGYKKGSFPR
                     AEAVVSEIVSLPLYPSLTEEEVQYVCESIHQFYGV"
     gene            2504..3655
                     /locus_tag="PK125_11250"
     CDS             2504..3655
                     /locus_tag="PK125_11250"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HPB38721.1"
                     /translation="MKVLQLFSDWKWTGPAEPVLSLCEALGTEGVDVAVAYRKTPIEF
                     PERTIEKEVKLRGLKFFDGFKLNRYFAPRDWFFDMRAIKAYVLQEQIDIVHANLSHDH
                     FTTLFSLGFSNKRPLIVRTDHKRDGTPYSYFMGWAFAKTDGLVAYGKKIMISDMKTFS
                     YPQERTCVIPPAIKTYNGPVKSLREEFGIKENEKIVCVIGRLKPDRGYDVILRGFREL
                     KNRVDGVKLLVVGRSSQIEKSIMKPISELGLENDVILAGYRVDDYFSVISTLDLFVMM
                     RAGSDGSARALREVMAMGKAAVVSDNGMLPELVQDGISGFVVKSDPIQLAEKMELILK
                     DEQKRIGFEENAKKVAAEEWNYTAQARKLTEFYERLLVLGKRPFPERNL"
     gene            3615..4802
                     /locus_tag="PK125_11255"
     CDS             3615..4802
                     /locus_tag="PK125_11255"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HPB38722.1"
                     /translation="MFWARGLSQKETCEAKDLKRFMKIGLAIYNFDPKKGGAERYAYD
                     LATDLLNRGHQVYVFCSKAVNLRGVVIAKFDTVRFPRWLRSLFFALNHRRYRKMFSLD
                     IMLGFGNTLDIDVYQSHGGVQRFWMEREIASYDDPREKRIKAALLRNSLNQRIQRWVE
                     EYPIRRGGYSRIVAISDMVKGHMKEHYSLKDDKIDVVYNGVDTEKFRPGDQKNTQKME
                     ILFSAGNFRLKGLLPLLTATGQLAKERSDFQLLIMGRGRKERYEETIGRLGIGNMVRF
                     LGETSSPEMIYRQAHALVHPTFYDACSLTTMESMASGLPTVTTRWNGASALIDLDEGY
                     VIEGPEDIEALTDAIRNLFNEEKRIVMGKKARLKLEEYTIVRNVDLMENIFQEVCDEK
                     HSQ"
     gene            4783..5415
                     /locus_tag="PK125_11260"
     CDS             4783..5415
                     /locus_tag="PK125_11260"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HPB38723.1"
                     /translation="MKNIHNEWSSLFLHRDKIHKVHREVWDIPLIKKRSHLLRGIIKD
                     NMTLLDIGAGMKGMKDEIANLGIKVTYKSMDVDRSNEHDYYEVSSINETFDAIIMFEV
                     IEHMSLQEGLEVMKKLFSIMNTKGIIVVSTPNIFNPARFMRDATHKTFYAYDELCGLL
                     SIAGFQIKNVYRSYNDAFHRYFMKVHLLGWLFRFLGIDYAFSIFVVGEKE"
     gene            complement(5561..5989)
                     /locus_tag="PK125_11265"
     CDS             complement(5561..5989)
                     /locus_tag="PK125_11265"
                     /inference="COORDINATES: protein motif:HMM:NF040320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HPB38724.1"
                     /translation="MFIKQISIVLDNVPGAMSHVSEILGREGVNIRAISVADTSDIST
                     VRFVVDDPVKAVNILKANGFSTKETDVLAVETPDHPGGLNAVLKPLKVAGINVHYLYP
                     HLGGVGGQAVVILGVDRLEEAQKVLQENWVKTLGKEVYTI"
     gene            complement(5993..6532)
                     /locus_tag="PK125_11270"
     CDS             complement(5993..6532)
                     /locus_tag="PK125_11270"
                     /inference="COORDINATES: protein motif:HMM:TIGR02175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HPB38725.1"
                     /translation="MLTKTIFSGFGGQGVLSMGYTLATAAMLEGKHVTYFPSYGVEVR
                     GGTANCTVAVSDEEIASPVASEPEFVVAMNQPSFARFQSILQSGGLIFVNSSIVDVSS
                     ARGDIEIVAVPTSELAERIGTIKVANMIMLGAFIRLTNIISYDFMVKNLTDILGEGKA
                     KLIKINKEALDLGYKYVRE"
     gene            complement(6526..7266)
                     /locus_tag="PK125_11275"
     CDS             complement(6526..7266)
                     /locus_tag="PK125_11275"
                     /inference="COORDINATES: protein motif:HMM:NF014794.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HPB38726.1"
                     /translation="MKKVYSRPKSLKPAHFHYCPGCGHSIVNRLIAEVIDEMNLGDKI
                     ICVAPAGCGMLIYNYFDVDCLESAHGRGAAVATGIKRVRPDNLVVSYQGDGDLAAIGT
                     AESFHAANRGENITIIFVNNGVYGMTGGQMAPTTILDQVTTTTPVGRISEKAGHPVRM
                     CEVLAVLDGTSYLERVAVHKPAAIVATKKALVKAFQHQMNKTGFSMVEILSPCPTNWK
                     MDPLQACKWVEDVMSKQFPIGLVKEVQC"
     gene            complement(7263..>7941)
                     /locus_tag="PK125_11280"
     CDS             complement(7263..>7941)
                     /locus_tag="PK125_11280"
                     /inference="COORDINATES: protein motif:HMM:NF028456.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB38727.1"
                     /translation="YRTIVLAPSSVQEIADLMPLAFDLAEKYRNPVVVLGDGMLGQMM
                     EPVEFDHIKKSEPVAKDYILTGAKGRPSRMVRSLIFDVKEQEEHNWKLARKFQRIEQN
                     EVRYETHLVDDAELVVIAYGTAARIAKGAIARLRQEGLKVGMIRPITLWPFPTKIIQE
                     TSKTIHDFFVFEMSAGQLVEDVKLSLEGKGRVHFYGRPGGVIPTPVELFRIISRHCYQ
                     IQRVKKS"
BASE COUNT         2255 a         1662 c         1859 g         2165 t
ORIGIN      
        1 agaaatccct gtattcggtg atggacacat cctccgggat ttcctctaca tcaaagacct
       61 tgtatcctgc atgctcatga ccgccattac tgatgaagca tacggaaagg tttttaatgt
      121 tgggaccgcc ataccggtaa gttttttaga cctcgcaaaa aagattgttg agatagcagg
      181 taccgggaag gtggcgcaca ctgaattcac tcaagaacga aaagaggtgg agcccggtga
      241 ttactacgct gacatatccc gcataaagaa gatcgtgaaa tggtttccaa aggtatccct
      301 ggaagaaggg ataaaacaaa caatcgattt ctacaggaaa aataagaagg agtactggta
      361 gtctgatgga tgagcagtct atccgtcctc ttgcgcggaa cggggttcac gaaaaggtta
      421 ttgcctatct gacagacaaa ccgcgggggc gtatccttga tgttcccact ggccaggggg
      481 cactggcaaa aaaactacac gaggtaggat ttaccgtttc atgttgtgac atcgaacctg
      541 cacagtttct tgtcaaaggg ctaactgttg agaaaggtga ccttaatgga aggctgccat
      601 atggcgacga agaatttgac tatgtctgtt ttctcgaagc aatcgaacac atggaaaacc
      661 catacaatgc tgttcgtgag gtgacaaggg tcttgaagcg agggggcacc cttgccctga
      721 gcacgccaaa ttacctaaat attgagcgac gaatgaagtt tctcataaca ggttttttta
      781 ccaagccagt ctcacacgag agatttctga atgcctgtaa agggaggacc tacggattgc
      841 atctgagccc cctggggtat actctgatca gattcgccct agaggcttca ggcttaaaaa
      901 ttgtgcattt aacctatgat atgaagaagc caaaaattat cttttttaag ccccttacat
      961 tgtttataag gttatataca agattctggt caaaagataa acagaaagaa tactggtttg
     1021 atgagacaac cagtaaagta attcttgaag gtgggaatac cctgatcatt tttgctgaaa
     1081 aagtctgaca gctcaataca gagtcaccca tgaagaataa ggttcaaagt ggaaacaaca
     1141 gatgatgatg tagtagaatc tctaacatat tatctttata aggtttgcag tattagttta
     1201 ctcatagccg gtggagagtt caacgatgaa tgtaaagatg ttcgatttag cgagggatca
     1261 taaggagata agaagcgaac tccttggaat caccgagaga atactaacaa atggtgaatt
     1321 cattctcagt aaagaagtgg aggccttcga agatgctttt gcacagtaca ttggggcaaa
     1381 atatgctgtt ggtgttggaa gtgggacaga tgccatcaag attgccggcc ttgcctatgg
     1441 cctcgagaag ggtgataaaa ttgtaacgac gccaaacacg tatattgcaa cagtcatgtc
     1501 gctctccctc catggaatta tccctgtttt ttgcgatgtt gagacaagta cttacaacat
     1561 ggatccagag cgtctcgaag atattttgag gaaggagaag ggcatcaaac tctgcatccc
     1621 agtccatctt tatggttatc cttcttcgtt ggatgagata gctgctattt gcaggcaaaa
     1681 cggagtaggt atcctggaag atgcgtgcca agcccatggt agcctctata aaggaaaaaa
     1741 ggtaggtggt tttggggatg tttcagcttt cagcttttat cccacaaaaa accttggctg
     1801 ctatggagat ggcggtatgc ttgtaaccaa ttcagattcc atttacaaaa acgcccttat
     1861 gctccgcaat catggccagc acgaacggca tgtccatgtg gttgaggggt acaactcaag
     1921 gcttgatgag ttacaggcag caattctcag gtacaaactg acaaagcttg atatatggaa
     1981 tgaaaagagg cgtaagattg cgtcatggta caagaagaaa ttaaaagatg tgccggtagc
     2041 tttacccgtg gaagagccat ggggttacca tgtttatcac ctgtttgtca ttagggctga
     2101 gaaacgggat gatcttatga aatatctttt ccataaaggc atcaccaccc ttatccatta
     2161 tcctatccca atccatctcc agaaaaccta ccagactctt ggatacaaaa aaggctcttt
     2221 ccccagggct gaggcagttg tttctgagat tgtttccctc ccattgtacc catctctgac
     2281 agaagaagaa gtgcaatacg tatgtgagag tattcatcag ttttatggtg tatgaaagat
     2341 atgaatattg gtaaatttga ttgtttagca gtggcacatt cagggttgag ggtccgacgt
     2401 cacacaattt gttcgcctat ttctattgtt ttttcaagct tttcattttt cattactcca
     2461 tgctatatgg gaaatgcggc tctatcccat ggtggtgttc gacatgaaag tgcttcagct
     2521 ctttagcgac tggaagtgga cggggccggc agaacctgtg ttgtcattat gtgaggcgct
     2581 cggtaccgaa ggtgttgatg tggccgttgc ctaccgaaaa acccccatcg aatttccgga
     2641 acgaaccatc gagaaagagg taaaactgag ggggctcaag ttttttgatg gattcaaact
     2701 taacaggtat tttgcaccaa gggactggtt tttcgatatg cgagcaatca aagcgtatgt
     2761 attgcaggaa cagattgata ttgttcatgc aaacctttcc catgaccatt ttaccactct
     2821 gttctcactg ggtttttcaa ataaaagacc tctcattgtc agaacagatc ataaacgtga
     2881 tggaacccct tatagttatt ttatgggatg ggcctttgcc aaaactgatg ggctcgtcgc
     2941 ctatgggaaa aagatcatga tttccgatat gaaaactttc tcttaccccc aggagagaac
     3001 atgtgtaata ccgccagcaa taaaaactta caacggacca gtgaaaagtc tcagagagga
     3061 atttggaatc aaagagaacg agaagattgt ttgtgttatc ggacgtctca agcctgaccg
     3121 aggttacgat gtcattctga gaggcttcag agaactcaaa aaccgtgtgg atggagtgaa
     3181 gcttctcgtt gtaggcagaa gctcccaaat agaaaagagc ataatgaagc ctatcagcga
     3241 actgggactt gagaatgacg tgattcttgc tgggtacagg gttgacgact acttttccgt
     3301 aatctctacc ctcgatcttt ttgttatgat gcgtgcagga agtgatggta gcgcaagggc
     3361 cctcagggaa gtcatggcca tgggaaaggc cgcggttgtt tcagacaatg gtatgcttcc
     3421 tgagctcgtg caggacggta tttcagggtt tgtcgtgaaa tctgacccca ttcagttggc
     3481 tgagaaaatg gagcttattc ttaaagacga acaaaagaga ataggttttg aagaaaatgc
     3541 aaagaaggtt gctgctgaag aatggaacta cacggctcag gccagaaagc tgacagaatt
     3601 ttatgagagg cttcttgttt tgggcaagag gcctttccca gaaagaaact tgtgaagcta
     3661 aggatttaaa aagatttatg aagataggcc ttgccatata taactttgat cctaaaaagg
     3721 gtggcgctga aagatatgcc tatgatttag ctacagacct cctgaataga ggtcaccagg
     3781 tatatgtgtt ttgctcgaaa gctgttaatc tccgaggtgt tgttattgcc aagtttgata
     3841 ctgttcgctt cccgagatgg ctgcggtcgc tcttttttgc actaaatcac cgaagatata
     3901 gaaaaatgtt ttcccttgat atcatgcttg gattcggaaa cacactcgat atcgatgtat
     3961 accaaagcca cgggggtgtt cagcgcttct ggatggaaag agagattgca agctacgatg
     4021 atcccagaga gaaaagaatt aaggcagcac ttctcagaaa tagtctcaac caacgcatcc
     4081 agcgatgggt tgaagaatat cctattagaa ggggcggata ttcacggatt gttgcaattt
     4141 ctgatatggt taagggtcat atgaaagagc actacagttt aaaagacgac aaaatagatg
     4201 ttgtttataa tggagttgat acagaaaaat ttaggcctgg agatcaaaaa aacacccaga
     4261 aaatggaaat acttttttcc gcagggaatt tcaggctgaa aggcctgctc ccccttctga
     4321 cggcaacagg ccaacttgca aaagaaagaa gtgatttcca gttgttgatc atgggaagag
     4381 ggagaaagga gcgctacgag gaaaccattg gacgcctggg tatagggaac atggtgaggt
     4441 ttcttggcga aacatcttcg ccggaaatga tttacaggca ggcccatgcg cttgttcacc
     4501 ctacctttta tgatgcgtgc tcactgacaa ccatggaatc tatggcgtct ggcctaccca
     4561 ctgtgaccac gcggtggaac ggcgcgtcag cccttataga tttggatgag ggttatgtca
     4621 tagaggggcc ggaagatatt gaggcattga cagatgcgat cagaaatctt ttcaacgagg
     4681 aaaaaaggat agtcatgggt aaaaaagcac ggctcaaact cgaggaatat acaatcgtaa
     4741 gaaatgtgga tcttatggaa aacatatttc aagaggtttg tgatgaaaaa cattcacaat
     4801 gagtggtcga gtcttttctt gcaccgggat aagattcaca aggtgcaccg cgaagtatgg
     4861 gatattcccc ttattaaaaa gaggtctcat ctcctaagag gcataataaa ggataacatg
     4921 accctcctcg atattggggc aggtatgaag gggatgaaag atgagattgc caatctcgga
     4981 atcaaggtta catataaaag tatggacgtg gacagaagca atgagcatga ttattatgaa
     5041 gtttcctcta tcaatgaaac gttcgatgcc attatcatgt ttgaggtcat cgaacacatg
     5101 tctcttcagg aaggccttga ggtgatgaag aaactgtttt caataatgaa cacaaaaggc
     5161 atcattgttg tctcaacccc gaatattttt aatccagcca ggtttatgag agatgcaacc
     5221 cataagacct tttatgccta tgatgaactt tgtggtctcc tcagtatcgc tggttttcag
     5281 atcaagaatg tatatcggtc gtacaacgac gcttttcacc ggtattttat gaaggttcac
     5341 cttctcggct ggcttttccg gttcctggga attgactacg ctttttcgat atttgtagta
     5401 ggggagaagg agtaatttgt cgggtattca gcgttcagtg actgttgaca tagcccaaag
     5461 attatagggc actgcgttca acgggatttc ctatgaactt aagataatga gccgatgttt
     5521 gttcccacta gtctcttgct ttttgtcaat ttttttgggt tcagattgta tatacttctt
     5581 tgccgagcgt tttgacccag ttctcctgaa gtaccttctg tgcctcctct aacctgtcaa
     5641 cgcccaggat caccacagcc tgtccaccaa ccccgcccag atgtggataa agataatgga
     5701 cattaatgcc ggcaactttg agaggtttga ggactgcatt gagtccgccg ggatgatctg
     5761 gtgtttcaac agcaagaaca tctgtttctt tcgtggaaaa tccattggcc ttaaggatgt
     5821 ttactgcttt gaccggatca tcaactacaa aacgcactgt gcttatatct gaagtgtcag
     5881 cgacagatat ggccctgata ttaacacctt cccgtcccaa tatttcactt acatgggaca
     5941 tagcccctgg tacattatca agtactatgg atatttgctt gatgaacatc cttcactccc
     6001 ttacatattt gtaccccaaa tcaagggcct ctttgttgat tttgatcaac tttgccttgc
     6061 cttcacccag aatgtctgta aggttcttaa ccataaaatc gtaggatata atattggtca
     6121 atctaatgaa cgcaccgagc atgatcatgt tggccacttt gattgtccca attctttccg
     6181 caagttcgct tgtaggaacc gcgacaatct cgatatctcc acgggcagat gatacatcca
     6241 caatggagga gttgacaaaa atcaaaccgc ccgactgcag gatgctttgg aagcgggcaa
     6301 aagatggttg attcatagca acaacgaatt caggttctga tgcaacaggt gatgcaatct
     6361 cttcatcgct aaccgcaacc gtacagtttg ccgtacctcc ccttacctct accccatagg
     6421 atggaaagta agtaacatgt tttccttcaa gcatcgctgc ggtagcgagg gtatatccca
     6481 tagaaagaac gccttggcct ccaaatccgg aaaatatagt tttagttagc attgaacctc
     6541 tttgacaagt ccgataggaa attgcttgct catcacgtcc tcaacccact tgcatgcctg
     6601 gagtgggtcc atcttccagt ttgttgggca tggagaaaga atctccacca tagaaaaccc
     6661 ggtcttattc atttggtgct gaaatgcttt cacaagtgct ttttttgtag ccacgattgc
     6721 agcgggttta tgaacagcta cacgttcaag ataggatgta ccgtcaagga ctgcaaggac
     6781 ttcacacatc cttactggat gtcctgcctt ctcggatatc cttcctacag gggttgtggt
     6841 ggttacctga tcaagaatcg tagtaggagc catctgtcct ccagtcatac cgtagacacc
     6901 attgttaaca aaaatgatgg tgatattctc tccccgattt gccgcatgaa agctctcagc
     6961 tgtgccaata gctgcgagat cgccgtcacc ctgataagag acgactaggt tatccggcct
     7021 tacacgctta atcccagttg caacagcagc accacggcca tgggcagatt caaggcaatc
     7081 aacatcaaag tagttgtaaa taagcatgcc acatcctgct ggagcaacac atattatttt
     7141 gtccccgaga ttcatttcgt caatcacttc agcaatgagg cgatttacga ttgagtgccc
     7201 gcatccgggg cagtaatgga agtgggctgg tttcagagac ttgggccggg aataaacctt
     7261 cttcatgact tcttgaccct ttgtatctgg tagcaatgac gggagattat acggaagagc
     7321 tcaaccggtg tcggtataac gccaccgggc ctgccgtaaa aatgaacacg gcctttgcct
     7381 tcgagagaga gttttacatc ttctactagc tgaccggcac tcatttcaaa gacaaagaaa
     7441 tcatggattg tctttgatgt ttcctggatt atttttgtag gaaaaggcca gagggtaatg
     7501 ggcctgatca tgccaacctt caaaccttcc tggcgaagcc gtgcaattgc acctttagcg
     7561 attcttgcag cggtgccata ggcgattacg accaactctg cgtcatcaac caggtgtgtc
     7621 tcataacgaa cctcgttttg ttcaatccgt tgaaattttc gtgccaattt ccagttgtgt
     7681 tcctcctgtt ccttaacatc aaagatgagg gagcgcacca ttctcgaagg tctccctttt
     7741 gcgcctgtca atatgtaatc tttggctact ggctctgatt tttttatatg gtcaaactca
     7801 actggttcca tcatctggcc gagcattcca tcgccgagga cgaccacagg gtttctatat
     7861 ttttccgcaa gatcaaaagc aagaggcatt aaatctgcta tctcctgtac cgatgaagga
     7921 gcaagcacaa tggtgcgata a
//
LOCUS       DAOOMC010000124         7840 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdus sp. isolate AMR_MDS_2685
            NODE_10894_length_7840_cov_5.183173, whole genome shotgun sequence.
ACCESSION   DAOOMC010000124 DAOOMC010000000
VERSION     DAOOMC010000124.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864760
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdus sp. (wastewater metagenome)
  ORGANISM  Syntrophorhabdus sp.
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae; Syntrophorhabdus.
REFERENCE   1  (bases 1 to 7840)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7840)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,729
            Genes (coding)                    :: 2,708
            CDSs (with protein)               :: 2,708
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 7 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7840
                     /organism="Syntrophorhabdus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10894_length_7840_cov_5.183173"
                     /isolate="AMR_MDS_2685"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2699756"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(201..1460)
                     /locus_tag="PK125_11285"
     CDS             complement(201..1460)
                     /locus_tag="PK125_11285"
                     /inference="COORDINATES: protein motif:HMM:NF025019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPB38728.1"
                     /translation="MSDDLLLFRFVETLLPSENVRSIAILTGARQTGKTTSAQRKYPD
                     LRYINLDAPENRDALRLLSTSAWAKTVGPAVLDEAQKEPVVFEKVKYAFDSQALSFSV
                     MLGSSQILLLKKVRESLAGRSSVFELWPLMMSEIHTIPGIAEKQSPLLDRLINGTDIS
                     KALRDEPELLLDAADEHCRAAEDYLLSWGGMPALLSLSPEERWQWLKDYEYTYLERDL
                     SDLARLDDLLPFRTFQKLAALRSGQMLNYSEIARDAGLSVDTARRYTEYLRLSYQTIL
                     IQPYYKNITSTLVKSPKIYWLDVGLLRHLTGIRVEVSGAIYETMVVGEIIKWIKTMRR
                     DVEVYFYRTRSGMEVDLLLVTQHGIIGVEVKARRSVAPPDWRALREIAERLGGDWLGG
                     LVIYRGSQIREVSEPGIWSIPSRRLFT"
     gene            2003..2854
                     /locus_tag="PK125_11290"
     CDS             2003..2854
                     /locus_tag="PK125_11290"
                     /inference="COORDINATES: protein motif:HMM:NF024258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HPB38729.1"
                     /translation="MRDAGRRYWWSILVLLIGIIVVWFYSKSFNVEVNHVYIKGYTSG
                     SGLTIAHISDLHIGKSDKQVVEILRILHGLKVDAIVLTGDYVEWNGDYEPALNVLAQI
                     KAKHGVYAVMGDYDYSNSRKSCLFCHERGTGKPTNRHQVKFLRNSCDEVAISKDTVMI
                     CGIDGRQANEPISPEVIKHVQDITRSKQAVVLSHSPLAFDMFDNDKDILVLAGDTHGG
                     QVPLPSWVWKFLGYEKNAKYEQGRFEEGKKKMYVNRGVGTSHLPIRFMRRPEITVFHF
                     GESGDRR"
     gene            3329..3889
                     /locus_tag="PK125_11295"
     CDS             3329..3889
                     /locus_tag="PK125_11295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB38730.1"
                     /translation="MQEKQVKLKYLCFLVVFLICQLGMGCSTSPGVRIIFDFETDEEL
                     DHFHWKCRTLFALSDEHVTHGSKSLKLELYPSEYPGLAPMIKDTDWRRYGAFQFDVYN
                     PEEKDIVLSVRIDDKEDYPDYADRYNNSFAIKPGANAITIGFDSLITSGTKRVLNLTM
                     IYRVIIFVAQPKEKTTLYFDYFRLVP"
     gene            complement(3935..4504)
                     /locus_tag="PK125_11300"
     CDS             complement(3935..4504)
                     /locus_tag="PK125_11300"
                     /inference="COORDINATES: protein motif:HMM:NF012271.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HPB38731.1"
                     /translation="MTPKNICRVFFAVTIISLFFMIGNGHCISIKIGWNPSGKGDPTG
                     YKIYYFCERQTGFEAFVIDVGDKTIKSLNLQKGYQYYIVVTAYNEYGESAPSNVLSVN
                     TCTYRLSPGKKTMKQTGGFSSVKVTTQPDCNWSAASGSDWLKIIDGESGKGRGVIRYV
                     VESNDTYEPRTVICTFAGKSFTVKQKGKK"
     regulatory      4948..5038
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            5103..7334
                     /locus_tag="PK125_11305"
     CDS             5103..7334
                     /locus_tag="PK125_11305"
                     /inference="COORDINATES: protein motif:HMM:NF039835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BACON domain-containing carbohydrate-binding
                     protein"
                     /protein_id="HPB38732.1"
                     /translation="MVTRKVSSQLLRCKSFRLTDLRLPGKRSTAHLCLFLFLILSSIV
                     FMQLVNTAHAGQVTLAWNKNAEENVVGYNVYYGTSSRNYDFVLDVGDTTTVTISDIPN
                     GFTYYFAVTAYDNSSPSLESTYSNEVSKNTCTYSISPTSANFGASGGTGSVSVTTQAG
                     CTWTASSGAAWLTITSGSSGTGNGTIRYSVSANTTPDTRTASSTFAKKVFTVTQSGAT
                     TYTIGAFAGTGGTISPSGSVNVAHGGSQTFTITPDTGYTIAGVTVDGVSVGAVSSYTF
                     SNVTANHTISATFTPSTYTITASAGTGGTISPSGSISVNHGASRTFSISANTGYTITD
                     VTVDGVSVGAVSSYTFSNVTANHTISATFSLKTYTISASAGTGGAISPSGNILVNHGG
                     NQTFTITPGAGYRIATVTVDGVPVGATGSHTFTNVMANHSISATFSVNTFTINASAGF
                     GGTISPAGTVTVEQGANQTFTFTANSGYAVSNVVVNGSSLGSLGSYTFTNVSSNHTIS
                     VSFAEGYTLNITKSGTGTGEVTTNPTATVFRPGTRVTLKAIKADSSLFSGFSGDCNTS
                     RTSCTIIMRKNANVNAAFKLKTFKVKTIVSGNGTVTVGEPVSQEEGAQAVKATKTEKI
                     KKSKHEATIDYGDQVTFTITPDPGNNIKKVVMNSRSIGVLDQLTIADIKRNYKVKVKF
                     ETDVAPSKSAGLETTNRQAVFQDESEQDGFASTAADMAENDGAERLIVKPAPAGHYAS
                     VKN"
BASE COUNT         2350 a         1603 c         1898 g         1989 t
ORIGIN      
        1 ggtaggaggg aaagaaaaag cacatagctg taagctcata gcaaagaata aaaaaggcga
       61 aaggtaagag gtaggaggta ggagggaaag aaaaaggtca tagctgtaag cgaagagaaa
      121 ggcagaaagc gcataaagac aaggaaaaag ctcatagctg agaataaaaa aggtacaggg
      181 ggaaagggaa gagagggagc ttatgtgaac agtcttcgtg agggaatgga ccagattcca
      241 ggctcgctga cctcccttat ctgagatcca cggtaaatga ccagtccacc aagccaatcg
      301 cctccaagtc tttcagcgat ttctcgcagc gccctccagt caggtggcgc tacagaacgc
      361 cgtgctttta cctccacacc gataatgcca tgttgagtta caaggagtag atcaacttcc
      421 atcccggatc gggttcgata gaaataaacc tcaacgtccc gcctcatagt ctttatccat
      481 ttaattattt cccccaccac catggtttcg taaatcgcgc ctgaaacttc tacgcggata
      541 cccgtgagat gccgcaagag accgacatca agccagtaga tttttggaga cttgactaac
      601 gtactggtga tatttttgta gtaaggctgg atgagaatcg tttggtagga aaggcgtaag
      661 tactccgtat atcttcgggc agtgtcgacg ctcaagcctg catctctggc tatctcggaa
      721 tagttaagca tctgtcccga acgcaaagca gcgagtttct ggaaggttct gaaaggaagg
      781 aggtcgtcaa ggcgcgcgag gtcactcagg tctctctcca gatatgtata ttcatagtct
      841 tttaaccatt gccagcgctc ttcaggggaa agcgaaagga gagcgggcat acctccccag
      901 gacagcaaat agtcttcggc ggcccggcaa tgctcatcgg cagcatcaag tagaagttcc
      961 ggttcatccc taagtgcttt gctgatgtca gtaccgttga tcaacctgtc aagaagtggt
     1021 gattgttttt cagcaatgcc tggtattgta tgaatttcac tcatcatgag cggccatagc
     1081 tcaaaaaccg aggatcgtcc tgccagtgat tcccgcactt ttttcaaaag aagaatctgg
     1141 ctcgatccca gcatgaccga gaaggacaat gcttgtgagt caaaagcgta ttttaccttt
     1201 tcaaatacca caggctcctt ctgcgcttcg tcgagaacag ccggcccaac agtctttgcc
     1261 cacgcggagg ttgataataa tctcaatgcg tcccggtttt ctggtgcatc gagattgatg
     1321 tatcgtaagt cggggtactt tctctgagct gacgttgttt ttcctgtttg cctggctccg
     1381 gtgagtatcg cgatgcttct gacattctcg gaagggagaa gagtctctac aaatctgaat
     1441 agcagtaaat catcgctcat aatagttaaa tatacaggca atatgacgat atgtcaagaa
     1501 tgaaatcgta gttttaaatt cactccgaca attcgacaga ctggtggatg ggaggaggaa
     1561 agaaaaagct cgtagctcat agctgtaagc tgatagcgaa gaggagaaaa aggaaaaagg
     1621 ttaaaggtag gaggtaggag gaaagaaaaa gctcgtagct caaagctgta agctcatagc
     1681 agaaaataaa aaaggcgaaa ggaaaaagct caaagctcat agctgtaagt tcattgcaaa
     1741 gagaaagaca gaaagatcat aggaaaaagc tcatagctca aagctgatag cgaagagaaa
     1801 ggcaaaaaga tcatagcaga gaataaaaaa ggtacagggg gagatggagt ctttgttaat
     1861 gaacaaatga tatggtgttt cataagagga tgaacacatt attgactgcg ctaaatcttc
     1921 aatgaggcta ttggcagggt ttatttttga caaaaaaaat gacggataca cgttgtaaga
     1981 gagaagactg acacaggcag gcatgagaga cgctggtcga agatattggt ggtctattct
     2041 ggtgttgttg attgggatta ttgttgtctg gttctatagt aaatcattca atgttgaggt
     2101 caatcatgtc tatattaagg gctacacctc tggaagtgga ttaaccattg cccatatttc
     2161 tgatctccat atagggaaaa gcgacaaaca agtggtggag atacttagaa tactgcatgg
     2221 tctgaaggtg gatgcaattg ttcttaccgg ggattatgtg gagtggaatg gggattatga
     2281 gcctgcgttg aacgttctgg cccagattaa agcaaagcat ggggtttatg ctgtaatggg
     2341 agattatgat tacagtaatt ccaggaaaag ctgcctgttc tgccatgagc ggggaaccgg
     2401 aaagccaacg aaccgtcatc aagtgaagtt tctcaggaat tcatgtgatg aagttgcgat
     2461 tagcaaggat acagtgatga tttgcgggat tgatggaagg caggcaaatg aacctatctc
     2521 tcctgaggtc ataaagcacg tgcaggatat tacacgcagc aaacaggcgg ttgtgctgag
     2581 tcatagcccg cttgcctttg atatgtttga caacgataag gacattcttg ttcttgcggg
     2641 ggatacccat ggggggcagg ttccgcttcc ttcctgggtc tggaaatttc ttggatacga
     2701 aaaaaatgca aaatatgaac agggccgatt tgaagaggga aagaagaaga tgtatgtgaa
     2761 caggggggta gggacgagcc atttgccaat cagatttatg agacggcctg agataacggt
     2821 gtttcatttt ggagaatcag gcgataggcg ataggttata ggaaagtcaa caggctgtat
     2881 cacgcccgct tcgctagaga cgcagagggc acaaagtaat agtaggtaag agggaagagg
     2941 taggagggaa ttgaaagctc gtagctcata gctgtaagct cataggaaac agctcatagc
     3001 tcatagctgt aagctgaaag caaagaggag aaaaaggaaa aaggttaaag gtaggaggta
     3061 agaggcgaaa ggcagggggg aagaaaaagc tcatagcaaa aaaaaggctg aaagctcata
     3121 gcagagaata aaaaaggcga aaggcaaatc agcaaccctg ctggtagctg aacaatcaat
     3181 cgccgcaggg caatgtgagt tttgccatga ttgctgatat ctctgaatca tagcaaaagg
     3241 atattctgat tctctctgta ttctccgtgg agagaaggag aaccgccagg gactgctggg
     3301 cgagaggaag ggatgtgtct gtaatcgtat gcaggagaaa caagtaaaat tgaaatacct
     3361 gtgctttctg gttgtttttc tcatttgcca gcttgggatg gggtgttcaa cgtctcctgg
     3421 tgtgaggatt atatttgatt ttgagacaga tgaagagttg gaccattttc actggaaatg
     3481 tcgaaccttg ttcgctctct cagatgagca tgtcacacat ggctcgaaat cactgaagct
     3541 ggaactctat ccatcggaat atccaggact agcgccaatg atcaaggata ctgattggag
     3601 aaggtatggt gcatttcagt ttgacgtata taatccagag gagaaggata ttgttttatc
     3661 ggttagaatc gatgacaaag aagattatcc ggattatgca gataggtaca ataatagttt
     3721 tgctattaaa cctggtgcaa atgcgatcac cattggtttt gattccttga tcacttcagg
     3781 aacgaaaagg gtgttaaacc tgacgatgat ttatcgtgtg attatctttg tggcccagcc
     3841 aaaagagaaa acaactttat actttgatta ctttcgattg gtaccatgag gaaaaatggc
     3901 cttcatgaac ggcaaaccat gctcctgatg atggtcattt cttgcccttt tgttttaccg
     3961 taaaggactt gcctgcaaac gtgcatatga ctgttcttgg ctcatatgtg tcgttcgatt
     4021 caactacgta acgaataacc ccccttcctt tccccgattc accatcaatt attttcagcc
     4081 agtctgatcc gcttgcggca gaccagttac agtcgggttg tgttgttact ttgactgagc
     4141 tgaacccacc ggtttgtttc atcgttttct tgcccggaga aagcctgtat gtacacgtgt
     4201 tcacagagag aacattggaa ggtgcactct caccgtattc gttgtaagcg gtaaccacaa
     4261 tgtaatattg atatcctttt tgtagattga ggcttttgat tgttttatct cctacgtcaa
     4321 ttacgaaagc ttcaaagccg gtttgtctct cgcagaagta gtaaatttta tatccagtcg
     4381 ggtcaccttt tcctgatgga ttccatccaa tctttataga gatgcagtga ccgttaccga
     4441 tcatgaaaaa gagcgatatg attgtaacag caaagaacac tctacaaata tttttgggtg
     4501 tcatatagaa attccaatgt aacacactca tagttaccta ttggagacaa aaaagcgact
     4561 gaggcttcaa ggcatgattg taccatactt tttgatttaa aagcacttac caacattttt
     4621 aacttcatgt attaattaac ttatagctgg aattatggtt tttgtcaaga aagtggggac
     4681 atgataacag acgatagcac atagctgaaa gctcacaggc aaaactggga aaatgatgtt
     4741 agttcatgtg gctcgttcgg tttgtggtgt gtacaaagac atgaccatgg cccatattcc
     4801 ccttcttttt ttgactcgta agccattgtg acctctatca tttacccata aatatgagga
     4861 atttacaata aagtcatgat tataatcgac agaccggaag gatttattgt acgtagcttt
     4921 tccggtttct gccgattgtt ctcaacagct tgaaaaaggc aaaccacgtg aaagcgtggg
     4981 gcgcaaagcc accggcctaa ctcccctgaa aacgggatat ggtagcgggg ttgccaggcg
     5041 aaggcagcac ctcatgtgct ggatttcgcg cggccaattg aatccagtag gaggtgttgc
     5101 tgatggtaac tcgaaaggtt tcatcacaac ttctaagatg taaatcattt aggttaacag
     5161 atttacgttt accgggaaag cgttcaaccg ctcatctatg cctgtttctg tttcttatcc
     5221 tctcatcaat cgtcttcatg caacttgtta atactgccca cgccggtcag gtcacactgg
     5281 cctggaataa gaacgccgag gaaaacgtgg ttggttacaa tgtctattac ggtacgtcaa
     5341 gcagaaatta tgattttgtc cttgacgttg gtgacacaac tactgtcacc atatctgaca
     5401 tcccgaatgg attcacgtac tacttcgcag tgactgctta cgacaattcg agtccgtctc
     5461 tcgaaagcac ttactctaat gaggtcagca aaaacacgtg cacctattcc atctccccca
     5521 ccagcgctaa ttttggtgca tcagggggca ctggaagcgt gtccgttaca acccaggcag
     5581 gatgcacctg gactgcttca agcggcgctg cgtggctgac cataacatca ggtagcagtg
     5641 gaacaggaaa cggcaccata cgctactctg tgtctgcgaa taccacacca gatacaagaa
     5701 cagcaagctc cacattcgca aagaaagtat tcacagttac ccagtcaggc gcaactacat
     5761 acacaatcgg ggctttcgca ggaaccggcg gcacaatctc cccttccggt tctgtcaatg
     5821 tagcccacgg gggaagtcaa accttcacca taaccccaga tacagggtac acgattgcag
     5881 gtgtaacggt tgatggagtc tcagttgggg cagtcagttc ttacactttt agcaatgtaa
     5941 cagcgaatca caccatttcc gccacgttca cacccagtac ctataccatc acagcttccg
     6001 ctggaaccgg tggcaccatc tccccatcag ggtcaatatc agtcaatcac ggagcaagca
     6061 gaacattctc catatctgca aatactgggt acacgattac agatgtaacg gttgatggag
     6121 tctcagttgg ggcagtcagt tcttacactt ttagcaatgt aacagcgaat cacacaatca
     6181 gcgctacctt ctcattaaag acatatacaa tcagtgcctc ggctggaacc ggcggcgcaa
     6241 tctctccgtc aggcaacatc ctggtaaacc atggcggaaa tcagactttc acaattaccc
     6301 cgggcgcggg ttacagaata gcaactgtga cagtggacgg ggtgcctgta ggagctactg
     6361 gttcccatac ctttacaaat gtcatggcaa atcactcaat ttcagcaacc ttttcagtaa
     6421 acacattcac tatcaacgca tcagctggtt ttggtggcac catatcacct gccggtactg
     6481 taactgtgga acagggcgca aaccagacat ttacatttac tgcaaactca ggttatgcgg
     6541 tttcgaatgt ggttgtaaat ggatcatcgc tgggctcttt aggttcatat acttttacca
     6601 atgtaagttc aaatcataca atcagtgtga gttttgcgga gggttacacc ctgaacatca
     6661 ccaagtctgg aacaggaaca ggggaagtca cgaccaatcc cactgcaact gtctttcgac
     6721 ctggcacaag agtgacccta aaggcaatca aggccgacag ttccctattt tcgggcttct
     6781 ctggagactg taacacctcc agaaccagtt gcacaatcat aatgaggaaa aatgcgaatg
     6841 taaatgccgc attcaagctg aaaactttca aagtgaaaac aatagtatca ggtaatggta
     6901 cagtgactgt aggagaacct gtttctcagg aagagggagc gcaagctgta aaagcaacga
     6961 aaacagagaa aatcaagaaa tcgaaacatg aagccaccat cgattatggt gatcaggtaa
     7021 ctttcacgat caccccagat cctggaaata atatcaaaaa agttgtcatg aatagccgat
     7081 ctatcggtgt tttagatcaa ttgaccattg ctgatattaa gcgcaactat aaagtgaaag
     7141 taaaatttga aacagacgtt gcaccgtcaa agagtgcagg gcttgaaaca acgaataggc
     7201 aagctgtttt tcaagatgaa agtgagcagg atgggtttgc aagcacggcg gctgatatgg
     7261 ccgaaaatga tggtgctgaa aggcttatcg taaaaccagc acctgcaggt cactatgcct
     7321 ctgtaaaaaa ctaagagcgt agcagactgg gtaaaattaa agcatccccc gggttacgaa
     7381 aatatttaat cagtgcggta acccggggga tgacaccacg tcgcatccat tgatcaagta
     7441 tggcatattg ttattacgcg taaagtcgag taattccgtt acaactgatt accaggtctg
     7501 ttattataaa ctcgccatgt gtgagttaag atccctttga tgggtaacca gattgaggta
     7561 cagtaataat aatggaagtg tcagcaccaa caatgattcc acaaggattt tgtaccaggg
     7621 ggctgaacga ggaagcagtc aactgtcata tggattgggc catacaggat tgtgcttccc
     7681 gctacaatgg agaggataaa ttatagttgt aaagacaaaa aggggtttcg accatattct
     7741 gaggttgtga aaatatccaa aaaattgatc atatcacctg cattttcagc ttttagaaag
     7801 attcaggtca gaatggtgga gaagaggaga ttttgagaca
//
LOCUS       DAOOMC010000125         7821 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdus sp. isolate AMR_MDS_2685
            NODE_10942_length_7821_cov_5.602241, whole genome shotgun sequence.
ACCESSION   DAOOMC010000125 DAOOMC010000000
VERSION     DAOOMC010000125.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864760
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdus sp. (wastewater metagenome)
  ORGANISM  Syntrophorhabdus sp.
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae; Syntrophorhabdus.
REFERENCE   1  (bases 1 to 7821)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7821)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,729
            Genes (coding)                    :: 2,708
            CDSs (with protein)               :: 2,708
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 7 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7821
                     /organism="Syntrophorhabdus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10942_length_7821_cov_5.602241"
                     /isolate="AMR_MDS_2685"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2699756"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(55..306)
                     /locus_tag="PK125_11310"
                     /pseudo
     CDS             complement(55..306)
                     /locus_tag="PK125_11310"
                     /inference="COORDINATES: protein motif:HMM:NF020052.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification enzyme R subunit
                     C-terminal domain-containing protein"
     gene            complement(407..913)
                     /locus_tag="PK125_11315"
     CDS             complement(407..913)
                     /locus_tag="PK125_11315"
                     /inference="COORDINATES: protein motif:HMM:NF016220.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease"
                     /protein_id="HPB38733.1"
                     /translation="MAVREYLTEAGPADYVLFVNKKPVGIIEAKKEEEGVRLTSHEQQ
                     SGEYAASKLKYLDNDPLPYVYESTGTLTHFTDYSDPKPRSRPVFTFHQPETFIEWQSR
                     GKSLRNKLHNLPSLPTQWLRDCQIRAINNLEKSLKENRDRVLIQMTTATKKPIRPNCH
                     GKSRNPGP"
     gene            complement(1163..1381)
                     /locus_tag="PK125_11320"
     CDS             complement(1163..1381)
                     /locus_tag="PK125_11320"
                     /inference="COORDINATES: protein motif:HMM:NF014663.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB38734.1"
                     /translation="MPSSVVVMVFSLKVANLFVGNDPGDAYPEATISGPELEFNAVTW
                     LVITGTNLDHHLNGPSIPMNTIVDVYCK"
     gene            complement(1523..2917)
                     /locus_tag="PK125_11325"
     CDS             complement(1523..2917)
                     /locus_tag="PK125_11325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB38735.1"
                     /translation="MKGSIIARKIGLLLIFITFSFSAFSQKGPLKVGAARVNVSGLAQ
                     LSIVPPTGKYTHEDLFVRAIVLDNGATRAVLISIDGNIRSEYDKISAMISRELNCPVE
                     NILLSGTHSHSAGLSESDEDAIIEVVKKAEASLRHAQVGFGEGKAWLNVNRDAIDKTT
                     RKWTQAANPDGPSDKTVAVMLFTDFDGNPIAGYMNYAMHPVNGYVTGIRSADYPGAAC
                     RHIEMSFGDDMVMMFTQGASGDQNPLWLRPGTNVIASRTGTKITGYEMTREVIESPLR
                     DYQIAKRRGLSLEGMTEHGAPTAETVDVLNRWIDALGMILGEEAIRVMTNINDFQTEV
                     KIRGARETLMMPGRVNINKEGEFKGEPGVFEDGPDVNVGIGVLGIGNIALANISAEIY
                     NIFGQKIKAASPLSKTIFVTLTNGGSNSGYIIDDASYNKNTFQVLGNKIQPGHAETKI
                     VESIVKLIEKHVED"
     gene            complement(2966..4138)
                     /locus_tag="PK125_11330"
     CDS             complement(2966..4138)
                     /locus_tag="PK125_11330"
                     /inference="COORDINATES: protein motif:HMM:NF012958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HPB38736.1"
                     /translation="MDVGTKPIEFPAINEDGTVSFRLRAPEAKTVVLVNTTGGWTTDA
                     WPEGSEVPMTKDAEGFWNLTIGPLEPELYNYAFIVDGVYALDPCNPLSTRDGVRYRSE
                     LPIPGEKTENYLYHDVPHGIVSHVYVPYPSLGIQKRTTIYTPPGYETGNERYPVIYLQ
                     HGGGGDEDAWTELGRVPEIMDNLIAKEQAVAAIVVMSNIYSDQVAGRNYIACVPPPGS
                     RPDDLSFPKALASDLVPYVDMTYRTLNNAEHRSVIGLSRGGMMSLYAAFANLDKFAWV
                     GSLAGGLPNFPGVAKVIDSPANPELFRGPDVSKTIEKEKLLELLPQIESAANDRLKLL
                     YISVGKQDGLVTAHRDFRDLLSAQGVEFDYYEVPGYGHEWAYWRVAWQDFAQKVFK"
     gene            4672..5283
                     /locus_tag="PK125_11335"
     CDS             4672..5283
                     /locus_tag="PK125_11335"
                     /inference="COORDINATES: protein motif:HMM:TIGR02985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma-70 factor"
                     /protein_id="HPB38737.1"
                     /translation="MEKLGSSAENQPLETTRMIDTEYFRKLYKEYYSALCVYATTFTR
                     DNQIAEEVVQDVFVKIWESGNTLSINTSISSYLFTSVKNASLNHLKHLQVVNRFSEYY
                     TRKYQNAQDLYYLSQETGDSLLIARELEEQLMKEIESLPDRCRKIFKMSRFENMKHQE
                     IADKLGVTVNTVHRQTSIALKRLKRIIIKSLALLVLSLKFLFA"
     gene            5366..6532
                     /locus_tag="PK125_11340"
     CDS             5366..6532
                     /locus_tag="PK125_11340"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR family protein"
                     /protein_id="HPB38738.1"
                     /translation="MTEIIVRVLKGEASPSDKQTLIAWLNESPENNESFRKLESLWNA
                     INIIDKSRVFDHEKAFLKFRKEVELKLKASKKARLVRIADYIIRIAAVAIILFGIGHL
                     IFSRPEEPFITEKAKCEIISPRGSKTQVILPDNSTVWLNSESKLEYNNEYGIASREVF
                     LEGEGFFEVKTNPEQVFVVNTACLRIKALGTTFNVKSYPSDNMVETTLIEGMVDLENI
                     TYGKPATIATLEPNQKVTYYKVTEKAGNEKKDRGITCDDANSSKVKDIIPILSNENVD
                     VSLVTSWTNNMIFFDHETFNDLAVRLERRFGVNIYFLDDEIGNLEFTGTISDIIIEQV
                     LEALQFAAPFYYQFYDKDIYISDKPILKKPSQETIINYPNTNLKTTMPMENKSN"
     gene            6681..>7821
                     /locus_tag="PK125_11345"
     CDS             6681..>7821
                     /locus_tag="PK125_11345"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HPB38739.1"
                     /translation="MKMSTFLLFFCLTAISAESFSQNFSFEIRGETIKDLLAKIERTS
                     DYKFLYRSDLTKINDPITVSISDENIESILTKVFAYSGLVFKIFDDNLVVISKANEFQ
                     QQSVTGRVTDATTGEPLPAVTVTIKGTTIGTLTDVNGSFIIPRPDRGMTISFSFIGYT
                     SYEVIYNDENTLEINLVQADLSLDEVIVVGYGTKTKSSVTGAISTVGEKELENIAVTN
                     VAGALQGRVSGAFVTSESGQPGAGSNIYFRGPMSISGGRPLYVVDGVPISDLSYDFNM
                     IDIESISVLKDASAAAIYGATAAGGVILVTTKRGAKQKPTVSFDGSYGVRNVIKVPTL
                     LQRDDYIMAKELAGYDVIDLFGPESEWNNLPDTDWFNEWYNPGAEQ"
BASE COUNT         2228 a         1738 c         1633 g         2222 t
ORIGIN      
        1 tgacagaaat tttaaagtta gtaaattcct gtccagtttt tctagaccat aacattatgc
       61 ggccagaaac tcattcatct tggcaattat cttctcagaa tctgatccga acaactgcca
      121 catcttatcc ttgccaccac tcgcatcgaa tggtgtataa tccaggtcat cataatcaag
      181 gtgaacactt gaagcaatat gatcttttat cttccttagc cagttcatct gctcctcatt
      241 aaacttgaga gctccggtct gtttcatgaa tatccagtct ttgaaattcc tgtcgacggt
      301 aaggtcaaaa ctggtaagac ctgtttccgg aggttataga tactacgatg acactcatcg
      361 atgattataa agtcaaaaaa ctctaccggg agtcttgggg tatagcttac gggcccgggt
      421 tccggctttt gccatgacag ttcggccgga ttggtttctt cgtcgctgtc gtcatctgga
      481 tcaggactct gtcgcgattc tctttcaggg atttttcgag gttgttaatg gctctgatct
      541 gacaatcgcg aagccattgt gtgggcagtg atggaagatt atgaagtttg ttacgcaatg
      601 atttacctct gctctgccat tcaataaagg tctctggttg atgaaaggtg aaaaccggac
      661 gtgatcttgg ctttggatca ctataatcag taaaatgtgt cagagtacct gtgctttcat
      721 atacataggg caggggatca ttatcgaggt acttgagttt gcttgcagca tattcgccag
      781 attgttgttc atgtgaagta agccgtacgc cctcttcctc ctttttcgct tcgattattc
      841 caaccggttt cttattaaca aacaggacat aatcagcagg tccggcttcg gtaaggtatt
      901 ctcttaccgc cacacccggc ccggcataaa ggttgatctc atttttttct ggatcaccca
      961 gccactctct ctgagggctt tgtcgatctt gtcccgtgca atctcttccg gagtctggtt
     1021 catttaaagg tctcagtata ttgctggtac caaggtaaag aataaaaaaa gaggaataaa
     1081 ggcataatta ggaactacta ttccacttgc ataagcaatg tttgttcagt gtccatcttt
     1141 tagacttatg aattcgagcc tattacttgc aatagacatc aacaattgta ttcatcggta
     1201 tacttggacc gttgaggtgg tggtccaggt tggtaccggt gattaccaac catgttactg
     1261 cgttgaattc aagttccgga ccggagattg tcgcctcggg atatgcatcg ccaggatcat
     1321 tgcccacaaa caggttggcc actttgaggg agaaaaccat caccacaacc gaggagggaa
     1381 tgccaaacct ctgatatcca cacctactcc ctgtcctgcg ccgagttaag caatctagcc
     1441 gtatgttaaa tcaacatcgt aataatcact ccttagggtt cggagttcgc ctgacttggc
     1501 gaggtcaaat ggtgagaaaa aactaatctt ctacatgttt ctcaatcagt ttcacaatac
     1561 tttcaacaat ctttgtctca gcatggccag gctgaatctt gttgcctaat acctggaagg
     1621 tatttttgtt atatgatgca tcatctataa tgtatccact gttggagcct ccgttggtca
     1681 aggtgacaaa aatggtcttt gacaacggtg aggctgcttt gatcttctga ccgaagatat
     1741 tgtaaatctc tgcgctaata ttcgcaaggg caatatttcc tatgccaaga acacctatac
     1801 ctacattcac atcaggcccg tcttcaaaaa cacccggttc acccttgaac tcgccttcct
     1861 tattaatatt cacgcgccca ggcatcatca gagtctctct tgcccccctg attttaactt
     1921 cagtttggaa atcattgatg ttggtcatca cccgtatggc ctcctccccg aggatcatgc
     1981 ccagcgcatc aatccaacga ttcaacacat caaccgtttc ggcagtgggg gcaccatgct
     2041 cagtcattcc ctccaacgag agacctctcc gcttagctat ctggtaatct cgtagcggcg
     2101 actcaataac ttccctagtc atttcatatc ctgttatttt agtcccggtc cgtgaggcaa
     2161 tgacattcgt cccaggcctt aaccagagtg gattctgatc gcctgacgca ccctgggtaa
     2221 acatcatcac catgtcgtca ccaaaagaca tctcaatatg acgacatgca gcacccggat
     2281 agtctgcaga ccgtatgccg gttacataac cgttgacggg atgcatagca tagttcatat
     2341 acccggcaat tgggttgcca tcaaagtcag taaataacat aactgcaact gtcttgtctg
     2401 aaggaccatc aggattggct gcttgggtcc atttcctggt tgtcttgtca atggcatctc
     2461 tgttcacatt caaccatgct ttaccttccc caaaaccaac ctgtgcgtgc ctcaatgacg
     2521 cttctgcttt ctttacaact tctatgattg catcttcgtc cgactcggaa agtcctgcac
     2581 tgtgcgaatg cgtccctgac aacaggatgt tctcaaccgg gcagtttaat tcccttgaaa
     2641 tcatagccga aattttatcg tattctgacc ttatgttacc gtcaatggat atcagcactg
     2701 cacgggttgc accgttatct agtacaatag ccctgacaaa caggtcttca tgggtatatt
     2761 tcccagttgg tggaactatc gatagctgcg ccagacctga aacgttaaca cgggcagcac
     2821 ccacctttaa gggacccttc tgcgagaacg ctgaaaagga gaaggtgata aaaattaata
     2881 acaaaccaat cttccgggcg atgatagaac ctttcatatt cttcttaatt catataaata
     2941 cttaaaaatc agacttctgt aaattttatt taaaaacctt ctgcgcaaag tcctgccatg
     3001 cgacacgcca gtatgcccac tcatgcccgt aacctgggac ctcataataa tcaaactcca
     3061 caccctgtgc agaaagaaga tcacgaaagt cacgatgagc agttaccagt ccatcttgtt
     3121 tgccaacact tatgtagaga agcttcagcc ggtcattggc ggctgattca atctgtggaa
     3181 gaagctccaa gagcttttcc ttttcaatgg tttttgagac atccgggcca cggaataatt
     3241 caggattagc aggagaatca atcacctttg ccactccagg gaagtttggc aatccacctg
     3301 ccagcgaacc aacccaggca aatttgtcaa ggttggcaaa ggctgcatag agtgacatca
     3361 tgcctccgcg agaaaggcca atgacagaac gatgctcggc attattcagc gtacgataag
     3421 tcatgtcaac atatggaaca agatctgatg caagagcttt tggaaaactc aggtcatcag
     3481 gacggctgcc gggaggaggg acacaggcta tataattacg gcccgccact tggtcggagt
     3541 atatattact cataaccact attgccgcta ctgcctgttc ttttgcaata aggttatcca
     3601 ttatctctgg cacccgcccg agctcagtcc aagcatcctc atcgccacca ccaccgtgct
     3661 gaaggtagat aacagggtat ctttcgtttc cagtctcgta cccaggtggt gtatagatag
     3721 tagtacgctt ctgtattccc aatgatggat aggggacata cacatgggaa acaatgccgt
     3781 gcggcacatc atgatatagg taattctccg tcttttcgcc ggggataggc aactcactac
     3841 ggtatctcac gccgtcacgc gtagataacg ggttgcacgg gtcaagagca tagacgccgt
     3901 ccacaataaa tgcatagttg tacaattcag gttcaagagg tcctatggtt aaattccaaa
     3961 atccctcagc atcttttgtc attgggactt cagatccttc aggccatgca tcggtagtcc
     4021 agcctccggt tgtgtttaca agaacaactg ttttagcctc aggagcccta agcctgaaag
     4081 atacagtacc gtcttcattg attgctggaa actctatcgg tttggttcct acatccatct
     4141 taggagtttc ctgtccgaag agatacctgc tgtttctaga tatttcatta cgggtgactt
     4201 tgccgcaccc tgatataatc accaaggctg cgctggcagc aaataatgca gttttaaata
     4261 tctttcccat cgattcaagg tatttatgtt ttcagttaat ctaaattcgc attctgtatc
     4321 ctttacaaat tatctcctga cactattctt gtttacccgt tccacaacct ctacaacctt
     4381 atcttacgta cccgggaatt ttattctcga tgcagtcata ccttgtattg atcttctcca
     4441 caaataatag acaaccaaca tcatctttac aataaaaagg tatagggata atgtatgaaa
     4501 cgggtgcctt aatggtcgat ctgcgcaacc cgggcattaa aaccaataat ggcatgatta
     4561 tgttttaccc attgttgaat ttattgtcat taaagaaatt atcagacatt tgtacaggat
     4621 gacgaatcaa tttaataact tgtgagcgaa ggagggaaag ggttatatca aatggaaaag
     4681 cttggttcat ctgctgaaaa tcaacccctg gaaactacca ggatgattga tactgaatac
     4741 ttccgtaagc tttataagga atattattct gctctttgtg tctatgcaac tacatttacc
     4801 cgtgataacc aaattgcaga ggaagtggtc caggatgttt ttgtaaaaat atgggagtct
     4861 ggcaacaccc tttccatcaa tacctcaatc agctcatacc tttttacatc ggttaaaaat
     4921 gcttccctta atcacctcaa gcatctacag gttgtcaaca ggtttagtga gtattatacc
     4981 cggaaatacc aaaatgcaca ggacttatac tatttaagcc aggaaacagg agactcacta
     5041 ttgatagccc gtgagctgga agagcaacta atgaaggaga ttgagtcttt acctgataga
     5101 tgtagaaaaa tctttaaaat gagtcgattt gaaaacatga aacaccaaga aatcgcagat
     5161 aaactgggtg ttacagtcaa cactgttcac cgccagacat caatagcttt gaagagactg
     5221 aagcggataa ttataaaatc gctagcatta ctggtattat ctctcaagtt cctttttgcc
     5281 tgaaaacttt tctcaaaaag atagaatcct cttttattgt ttacatggtc cttgttgtca
     5341 ttaatatgta gttgatatgg agaagatgac tgaaataatt gtgcgtgtgc ttaagggcga
     5401 agcctcacct tctgataagc agacacttat agcctggcta aatgagagcc cagagaataa
     5461 tgagagtttt agaaagctgg aatccctatg gaatgccatc aacatcattg ataaatcaag
     5521 ggtatttgat cacgaaaaag ctttcctgaa gttccgcaag gaggtcgagt taaaactcaa
     5581 agcttccaaa aaggcaagac tggtcaggat tgctgactac atcatccgta ttgcagccgt
     5641 tgcaataata ttatttggga tcgggcacct gatcttttct aggcctgagg aacctttcat
     5701 tactgaaaaa gcgaaatgtg aaataatttc accgagaggc tccaaaacac aggttattct
     5761 cccggataac tccactgtat ggcttaattc ggagagtaag cttgaataca ataatgaata
     5821 tggtatagca agcagggaag tcttcctgga aggagaagga tttttcgaag ttaagaccaa
     5881 cccggaacag gtatttgttg tcaatacggc ctgtctgaga attaaggcac ttgggacgac
     5941 cttcaatgtc aagtcatatc cctcagataa tatggttgaa accactttga ttgaaggaat
     6001 ggtggacctt gagaacatca catacgggaa acctgccact attgccacgc tggaaccgaa
     6061 ccagaaggtc acctattata aggttactga aaaggctggt aatgagaaga aagatagagg
     6121 gattacctgt gatgatgcaa attcttcaaa ggtaaaggat ataatcccga tactttccaa
     6181 cgaaaatgtg gacgtttcgc tcgtgacttc ctggactaat aacatgatct tttttgacca
     6241 tgaaaccttt aatgatctgg ccgtaaggct tgagagacgg ttcggggtaa acatttattt
     6301 ccttgatgac gaaataggga atcttgagtt cacaggaaca atcagtgata taattattga
     6361 gcaggtactt gaggcactcc aattcgccgc acccttctac tatcagttct acgacaagga
     6421 tatttacatc tcagataagc ctattcttaa aaaaccctca caggagacaa ttataaatta
     6481 tccaaacact aacttgaaaa ccactatgcc tatggaaaac aaatcaaact gaaaacgaat
     6541 cgggaaatgc tgcaacattt cccgaccatt gtttttaatt aaaccaggga gtacatttta
     6601 ctaacaggcc tctctggatc atttaaccaa aaacatacaa atttatgaaa aaagctcgat
     6661 ttaggaaaac tctgctaatc atgaaaatgt cgaccttttt attgttcttt tgcctgactg
     6721 caatttcagc cgaatcgttc tcgcaaaact tttcctttga aatcaggggc gaaacaatta
     6781 aggacttatt agcaaagata gaaaggacaa gtgactacaa gttcctctac aggtcggacc
     6841 tgactaagat taatgatcct attactgtca gtataagtga tgagaacatc gaaagcattc
     6901 ttaccaaggt ctttgcatat tccggtttgg tctttaagat tttcgatgac aatctggttg
     6961 tcatttcaaa agcgaatgaa tttcagcaac aaagtgttac cggtagggta acagatgcca
     7021 ctaccgggga accgctgccg gctgttactg ttacaattaa gggtaccacc ataggtactc
     7081 tgacggatgt aaacggaagc tttatcattc ctaggcctga taggggtatg actatatcat
     7141 tctccttcat cggatatact tcctatgagg ttatttataa cgatgagaat acattggaaa
     7201 taaatcttgt acaggcagat ctttcccttg atgaggtaat tgttgttggt tacggaacca
     7261 agacaaaaag ctctgtaact ggagcaattt caacggttgg agagaaagaa cttgagaata
     7321 ttgccgttac caacgttgca ggtgccctgc aaggacgggt ctcaggggca tttgtaactt
     7381 ctgagagcgg tcagccggga gctggctcaa atatttattt ccgtggcccg atgtcaatca
     7441 gtggtggtcg tcctctttat gtcgtggatg gtgtccctat aagtgatctc agttatgatt
     7501 tcaacatgat tgacattgaa tcaataagtg ttcttaagga cgcctctgca gctgctatct
     7561 atggagcaac tgctgcaggc ggtgttatcc ttgttactac aaaacggggt gccaaacaaa
     7621 aacccacggt aagctttgac ggcagctacg gggtccggaa cgtgattaag gttccaaccc
     7681 ttcttcaaag agatgattat attatggcaa aggagcttgc aggatatgac gttattgatt
     7741 tgtttggacc ggagagtgaa tggaataatt tgcctgatac tgactggttt aacgagtggt
     7801 ataatcctgg tgctgagcag a
//
LOCUS       DAOOMC010000126         7814 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdus sp. isolate AMR_MDS_2685
            NODE_10954_length_7814_cov_5.809383, whole genome shotgun sequence.
ACCESSION   DAOOMC010000126 DAOOMC010000000
VERSION     DAOOMC010000126.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864760
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdus sp. (wastewater metagenome)
  ORGANISM  Syntrophorhabdus sp.
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae; Syntrophorhabdus.
REFERENCE   1  (bases 1 to 7814)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7814)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,729
            Genes (coding)                    :: 2,708
            CDSs (with protein)               :: 2,708
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 7 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7814
                     /organism="Syntrophorhabdus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10954_length_7814_cov_5.809383"
                     /isolate="AMR_MDS_2685"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2699756"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..224)
                     /locus_tag="PK125_11350"
     CDS             complement(<1..224)
                     /locus_tag="PK125_11350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB38740.1"
                     /translation="MMNMKIARIAFQNLFSHPCLFCLVTLLLVFPVTLVHSGAIELPQ
                     TGQITCYSSSGNPIPCDNTGQDGDFIAGVS"
     gene            complement(466..1032)
                     /locus_tag="PK125_11355"
     CDS             complement(466..1032)
                     /locus_tag="PK125_11355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB38741.1"
                     /translation="MCSLYIVKKIAVDIDNILWDFSPVFWERLRRINPDIIPPTVWNR
                     WDFWHQYVTTKQLYGAIKEIHLEQEKFRPFDDASLFLSSLRDMGFYIIIASHREKGTI
                     EPTERWLGMYRLVYDEIHLSSDKSVLFNDLCAVVDDSPSVLDKAKAAGIVRAGLRCPW
                     NKDTDHPLFDTLPEILSYLKSVCNTAHG"
     gene            1157..2503
                     /locus_tag="PK125_11360"
     CDS             1157..2503
                     /locus_tag="PK125_11360"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HPB38742.1"
                     /translation="MTLSLRTFLSAAVILSVTFIPFFAAHAERLTLSDGIRMVTEDNM
                     LVKIRKQDELISESDTLIARSGLLPHVYGTYSQNYNEKQQGTQIQGQSAYTQQQDFYA
                     FSLRAQQLLWDFKGVLSIYEASKKIQEARHLEYKRTRNYVALSFALGYFDLLESEKMI
                     TVSEKETEMLSAHLTMAKNLYTEGVITKNDLLQADVRLSDARQKLLAAKNIRKITASR
                     LNTMLGRPLSSVLDPEEIPVRAPEPPIDLNWAMAKAEKDRYEIRMVDVTVDAIKFEEV
                     AKKAEYYPRFFVEGGYNYLRNKYALYDGSWSVMGGMSINLFSGGATLAGLDKIRREKD
                     KLTIERRKLIDDIRFEVERYYLELTSAREKVRVTKDATSQAEENLRINRVKYREGEGT
                     ATDVVDAITLLTVAETNHYRSQYELLRAEANLIHAMGRDLVEVYKELNGRVSEHRQ"
     gene            2475..3569
                     /locus_tag="PK125_11365"
     CDS             2475..3569
                     /locus_tag="PK125_11365"
                     /inference="COORDINATES: protein motif:HMM:NF024112.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HPB38743.1"
                     /translation="MEESANTVNNERKKRSLIIGGIIGCILICIIVAGYLYYRATRVS
                     TDDAFVEGRVHTIASKIAGTVKTVRVGDNQAVKKGDILVEIDEIDYDIRVKEALTALD
                     TEKTRLFEAESKLDAAKKQVEEMKAAVNSARANEELHEATARQAGIDMNRAENLFRNE
                     AISRERFEKTKTGLDVAEAQLKTAREQVKRLEASLAAQVSLMKQAEAVIRTQSAAIKQ
                     RQTMVEAADLNKSYTKIVAPSDGYVTKKSVEVGNQVSAGQPLLAVVALDDLWIVANYK
                     ETQLQKIRPGQKVKISVDAHPNAKLSGVVESIMAGTGAIFSLFPPENATGNFVKVVQR
                     IPVKIVLDTDANRENILRIGMSVVPTVYIR"
     gene            3569..5098
                     /locus_tag="PK125_11370"
     CDS             3569..5098
                     /locus_tag="PK125_11370"
                     /inference="COORDINATES: protein motif:HMM:TIGR00711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HPB38744.1"
                     /translation="MNRWLVAVTVMLPTLIEIIDMSVVNVALDHIRGSLSAGIDEATW
                     AITMYLVSNAIIIPITGWLSRYFGRKVYLTFSIALFTVSSLLCGMAWNIQSLIVFRIF
                     QGLGGGALQPISQSILLETFPPRLHGVAMAVFGIGIMFGPIAGPVLGGVITDYWSWHW
                     IFFINIPVGIISIFMTMLVIKDPPYMKKTRMKLDYPGLLLVALGLGCLQIILDRGQRE
                     DWFESSMISWLTIISVVSLALFLIVELRTKEPVIDLRVFRNISFATGNVIMFFAFFNL
                     FGSIVLLPIYLQTLMGYTATLAGLVLGPGGFSSIIALSIAGRLVTRINPKWILAFGIS
                     TAALSTYMMSQFNLSADFITVMWPRIILGFGMGFIFIPLTNLSLSIIKKEGMGNATAL
                     FNLIRNLGGSFGIAFVSTVLARRAQFHQSNLVSHLTPFDMPYQIAVEKLKAFFHTLGF
                     TDTMAKMAADGSIYRNLIKQASMIAFNDTFWLMSIMLVATLPLLLIFKRPRHGEHMET
                     M"
     gene            5511..6080
                     /gene="msrA"
                     /locus_tag="PK125_11375"
                     /pseudo
     CDS             5511..6080
                     /gene="msrA"
                     /locus_tag="PK125_11375"
                     /inference="COORDINATES: protein motif:HMM:NF013766.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
     gene            complement(6332..6535)
                     /locus_tag="PK125_11380"
     CDS             complement(6332..6535)
                     /locus_tag="PK125_11380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB38745.1"
                     /translation="MVLHIQYQNNKFDYINSEILDQFIHKRKIKRFYRPSEERWVVIG
                     IDPIRGNGGDHDGRERRRTLNPA"
     gene            6886..>7814
                     /locus_tag="PK125_11385"
     CDS             6886..>7814
                     /locus_tag="PK125_11385"
                     /EC_number="3.1.11.5"
                     /inference="COORDINATES: protein motif:HMM:NF016169.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit gamma"
                     /protein_id="HPB38746.1"
                     /translation="MAGLKLYTSNRLESLLDALASVVKEPLSSALEREVIVVQSKGME
                     RWLSMELAKKFGIWMNCRFPFPNKFVWEIFTIFIHDIPDILLYAPQVTTWRIMKLLPD
                     YLHAPGFDVISNYLVQKTDHLKRLQLSERIADVFDKYSVYRPEMVLRWDKGSHGSGQS
                     ERWQAELWRGLFKNGEEKHRAGILRDFLKAITYAKDSVTGLPKRVSVFGIPALPRYHF
                     EVIHALALVTDVHLFLLSPTKEYWADIVPDKKIATQPVGKDLHFEVGNQFLASTGRLG
                     RDFFSMLVSQSYEEFPYFKDPEGESLLARLQSD"
BASE COUNT         2204 a         1671 c         1920 g         2019 t
ORIGIN      
        1 caggaaactc cggctatgaa atcgccatcc tggcctgtat tatcgcaagg tatgggatta
       61 cccgagctac tgtagcatgt gatctggccg gtctggggaa gttcgattgc tccgctgtga
      121 acaagggtaa caggaaacac taataagaga gtaacgagac agaacaaaca agggtgtgaa
      181 aaaagatttt gaaaagctat tcttgcgatt ttcatgttca tcattgtgac cccctcgccc
      241 tgataggcca acattgtgtt tccattatac cacctaaatt attgctgtaa ggattcctct
      301 tgtcaatagc taaatgtgtt cactgagccg gtcggcggga agggcagggc gagcgggaaa
      361 agaagtaaga agtgaaaaat gaaaagttaa aagaaaaagt ctatgcaata ggcgatatcc
      421 aatgggcaat aagcagcaac aactttggaa gcgttcttac agctgtcagc catgagctgt
      481 attgcacact gatttgagat atgagagaat ttcaggcagt gtatcaaaaa gaggatgatc
      541 ggtatctttg ttccaggggc agcgcaaccc agccctcaca atgccggcgg ctttcgcttt
      601 atccagaacc gaagggctgt cgtcaaccac tgcacacaaa tcgttgaaaa gcactgattt
      661 atcactgctc aggtgaattt catcataaac aagcctgtac atgccaagcc aacgctctgt
      721 gggttcgata gttcctttct cgcggtggct tgcaatgatg atgtagaatc ccatatccct
      781 cagagacgag agaaaaagag atgcatcatc gaaaggcctg aatttttcct gttccagatg
      841 tatctctttt attgcgccat acagttgttt cgtcgtaacg tattggtgcc agaagtccca
      901 tctgttccat acagtgggag gaattatatc cggattaatt ctccggagcc gctcccaaaa
      961 aacaggagaa aaatcccaga ggatattgtc gatatcaact gcaatctttt tcactatata
     1021 taaagaacac attcagacga cctgtgtaaa tatggttcag gcttccgggc caccctcctc
     1081 agtctatctg tgattgacaa gaccccctgc aacagtatac attgtttgag ttttttaact
     1141 gttgaggata tccaatatga ccctctcgct tcgcacattt ttgtctgcag ctgttatcct
     1201 gtctgttaca ttcattccct tttttgcagc ccatgcagaa aggttgaccc tttctgatgg
     1261 catcaggatg gtcacagaag acaatatgct tgtaaagatc agaaagcagg atgagctgat
     1321 ttcagaatcg gacaccctga ttgcaagatc aggactttta cctcacgtgt atgggaccta
     1381 ttcacaaaat tacaacgaga agcagcaggg gactcaaatc caggggcagt ctgcctacac
     1441 acaacagcag gatttttacg cattctcatt aagagcccaa cagcttctct gggacttcaa
     1501 aggcgtcctg tccatctatg aggcaagcaa aaaaattcag gaagcgcgac accttgaata
     1561 taaaagaaca agaaattatg tggccctgtc gtttgccctt ggttattttg atctccttga
     1621 gtcagaaaaa atgatcaccg tgtcggaaaa agagacagaa atgctcagtg cccatctcac
     1681 aatggctaaa aatctctaca ccgagggtgt tatcacaaaa aatgatcttc tccaggcaga
     1741 tgtgaggctt tccgatgcca gacaaaaact cctcgcagca aaaaatatac gaaaaataac
     1801 tgcttcaaga ctgaacacaa tgctcggcag accgctgtct tctgtgttgg atccagaaga
     1861 aataccagta agagcaccgg agccacccat agatctaaat tgggccatgg caaaggctga
     1921 gaaagaccga tacgaaatac gtatggttga tgtgacagtc gatgctatta aatttgagga
     1981 agtagcgaaa aaggctgagt attatcccag gtttttcgtg gaaggggggt ataattatct
     2041 tcgtaataag tatgcccttt acgatggttc ctggtctgtt atgggtggaa tgagcattaa
     2101 tctgttcagt ggaggcgcaa cattggccgg gcttgacaag atcaggcggg aaaaggataa
     2161 gctgaccatt gagcgtcgga agctgatcga cgacataagg ttcgaggtgg aacggtatta
     2221 cctggaactt accagcgccc gcgagaaggt cagggtaaca aaagacgcca catcccaggc
     2281 cgaagagaat ctgcgcatca acagagtgaa atacagagaa ggcgagggaa cagcgacaga
     2341 tgtggtagat gccattacac ttcttactgt ggctgagaca aaccattacc ggtcgcaata
     2401 cgagctgctc agggcagagg caaacctgat ccatgcaatg ggcagggatc ttgtggaggt
     2461 atataaagaa ctgaatggaa gagtcagcga acaccgtcaa taacgagaga aaaaagaggt
     2521 ccctcataat cgggggtatc atcggctgta tcctgatatg cattatcgtt gccggctatc
     2581 tgtactacag ggcgacccgt gtctcaacag atgatgcttt tgttgagggg agagtgcata
     2641 ccatagcgtc aaagattgcg ggtacggtta aaaccgtgcg ggttggggat aaccaggctg
     2701 ttaaaaaggg cgatattctc gttgagatcg atgaaataga ctatgatatc agggtaaaag
     2761 aggcattaac agccctggac acggaaaaga caaggctttt tgaggcagaa agtaagctgg
     2821 acgctgctaa aaaacaggta gaagagatga aagctgctgt taactcggca agggcaaatg
     2881 aagaactgca tgaagcaact gcacgacagg caggtataga catgaatcgg gcagagaacc
     2941 tcttcaggaa tgaggcgatt tcccgtgaaa gatttgagaa gacaaagaca gggctggatg
     3001 ttgcagaggc tcagctcaaa actgcacggg aacaggtaaa gcgcctcgaa gcctcactcg
     3061 ctgcgcaggt ctctctgatg aagcaggctg aagctgtcat caggactcag agcgcagcaa
     3121 tcaaacagag gcagaccatg gttgaggctg ctgacctcaa caaaagctat acaaagattg
     3181 ttgctccttc agacgggtat gtgacaaaaa agtcggtgga agtggggaac caggtcagtg
     3241 ctgggcagcc gcttcttgct gtggtggctc tcgatgacct ctggattgta gcgaattaca
     3301 aagagactca gctccaaaaa atcaggcccg gacaaaaggt gaagatttct gtagacgctc
     3361 atccaaatgc aaaactaagc ggtgtagttg agagcatcat ggcagggaca ggtgctatct
     3421 tttccctttt ccctcctgag aacgccacag gtaactttgt gaaggtcgtc cagaggatac
     3481 ctgtaaaaat tgtgctcgac acagatgcca acagggaaaa cattctccgt attggcatgt
     3541 ctgttgtacc aactgtgtac atccgctaat gaacagatgg cttgtcgcgg taactgtcat
     3601 gttgcccact cttatcgaga taatcgatat gtcagtggtg aatgttgccc ttgatcatat
     3661 ccgtggaagc ctgtctgctg gtattgatga agccacctgg gccatcacca tgtaccttgt
     3721 ttcaaacgca attatcatac ccattacagg gtggttgagc agatatttcg gaagaaaagt
     3781 ctatctcact ttttccattg cccttttcac agttagctcc cttttgtgcg ggatggcctg
     3841 gaacatccag agccttattg ttttcaggat ttttcagggc ctgggcggag gagcccttca
     3901 gcctatttcg caatccattc tccttgagac ttttccccca cgcctgcacg gtgttgccat
     3961 ggctgttttc gggatcggga ttatgttcgg acctatagcc ggacctgtcc tcggaggcgt
     4021 cattacggat tactggtcgt ggcactggat attttttatc aacatacccg ttggcatcat
     4081 ttccatattc atgaccatgt tagttatcaa ggacccccca tacatgaaga aaaccaggat
     4141 gaagctcgat tatcccggtc ttctgctggt ggctcttggc ctcggatgcc ttcagataat
     4201 tcttgaccgt ggtcagagag aggactggtt tgaatcctcc atgatcagct ggctcaccat
     4261 catatcggtg gtgtctcttg ctctttttct tattgtggag ctgcggacaa aagaaccggt
     4321 tattgacctg agggtgttca ggaacatctc ttttgcaacc ggaaatgtta ttatgttttt
     4381 tgcttttttc aacctcttcg gaagcatagt tttacttccc atctatctcc agacgcttat
     4441 ggggtataca gcgacgcttg cgggccttgt gctgggtccc ggcgggtttt cctcgatcat
     4501 tgccctttcc attgcggggc gactggtgac aaggatcaat ccaaagtgga tccttgcttt
     4561 tggcatctcc acagcagccc tgtcaaccta tatgatgtct cagtttaacc tatccgctga
     4621 tttcataaca gtcatgtggc caaggattat cctcgggttt ggcatgggat tcatttttat
     4681 tccccttacg aatctttcac tctccatcat aaaaaaagaa gggatgggca atgcaaccgc
     4741 tctgttcaat ctcataagga atcttggcgg gagctttggg attgcctttg tgtctacggt
     4801 ccttgcacga agggctcagt ttcaccagtc aaaccttgtg agccatttga ccccctttga
     4861 catgccttac cagattgcag tggaaaaact gaaagcattt ttccacaccc tgggctttac
     4921 agacaccatg gctaaaatgg ctgcggacgg gtccatatac cgcaatctca taaagcaggc
     4981 aagcatgatc gcattcaacg acaccttctg gcttatgagc ataatgctcg ttgcaaccct
     5041 gcccttgctc ctaatcttca aaagaccccg ccacggtgaa cacatggaaa cgatgtgaca
     5101 aggggtggga taagctgtaa tgtggatcag tgacacataa gaggctgaaa aacagattgc
     5161 gcgtcacgtg gtccaggttg gggattaatc aggggttgct tggtcaggga acagaagtta
     5221 gcagtgtctc ccagttttga attacagcaa atatgcttat attaatggtc atgatatttt
     5281 caagaggcag gtcaggatat tggtcatggc ggattatttt gttaatcttt ctggtttctt
     5341 ctctgcaatc atgtggaatc acaggaagtg atacagaggt aaaggagaaa agagtgggat
     5401 ccggagaaac aggagcagca tataaaaaag aggcggtaac catgaacgaa gcgagggggg
     5461 ttaaaacaga gatagctact tttgcactcg ggtgattttg gggtccggat gcccggttcg
     5521 ggcatgtgga tggtgtaata cgcacccagg ttggttatgc aggtggaacg aaaaagaatc
     5581 ctacctatta caatcttgga gatcattcag agacaatcga gatcgaattt gatcctcaaa
     5641 aggtcacgta caggcaactt attgatatct tttgggaaag tcatgaacca acgtcccgac
     5701 ccttttctcg tcagtatgca tcgatgattt tttaccattc agatgaacag aaaagaatgg
     5761 cggaggagac aaaagaacag gcagaggcga aaaaagggca aaaggtgtac acagagatta
     5821 ttcctgccag tacgttttat ccggctgagg actatcacca aaaatactac atcaggcgct
     5881 atgatgggtt ggtaaaagaa ttgagcacac tttacgcggg caacagcaat tttacgaaat
     5941 caactgctgc tgtaaagatc aatggatatc ttggtggtaa tggaaccctt gaagagctaa
     6001 agaatcaatt gaaaaacctt gatttgtcac cggcggttat tgatcgtatt attgttttgc
     6061 tcagaggggc aagcaggtga gaaaagggcg gtaaggaagt aaaagttaga tgtgaaaagc
     6121 gagaagtaag gacaaaatgt cggaaagaga gcgtcgaggg ggggaacgag ggctggtgca
     6181 aaaaaaggga gggtgaaaaa tcagggttaa gggtcagagg ggtaagaggt taaatggagc
     6241 aaattcgtgg actggttcaa agcgcttaat atgaaaaaga aaccggataa accttttcaa
     6301 ccagaccatt agagagttcg tcaaaagctg ttcaggcggg attcagggtc cgtcgtcgtt
     6361 cccgtccgtc atgatcacca ccatttcccc gtatagggtc tattccaata acgacccacc
     6421 tttcctcaga gggacgatag aacctcttta tttttctctt gtggataaac tggtccagaa
     6481 tttcactgtt tatgtaatcg aacttgttgt tttgatactg tatgtgcaaa accatatgtc
     6541 tccccttcgt acggtagcac atgactatat ttatcaatta ttataccata aaagacgcaa
     6601 attgtggcaa aaatggtccc gaaagaaaac aagaagtctg tgcagcagtt tttgtgggtg
     6661 ccattgccca tgtttaaaga ctttgtgctg gttcttaaat aggataatgt ccttggccct
     6721 gccaggaaca gcatactgct cttccattct gatgcagaaa acagaatgat tgctcccggg
     6781 aaggtggaag aatacccacc attcaggaag gccttgggac agtcaatctt ttcccttctc
     6841 accctgtcgt cctatctggt agaataaaga agaacttaaa aacttatggc tggtcttaaa
     6901 ctctatacga gcaaccgtct cgaatcgctc ctcgatgccc tggcatctgt tgttaaagaa
     6961 cccctttctt cggctcttga gcgagaagtc attgtggtgc agagcaaggg tatggagcga
     7021 tggttgtcaa tggagcttgc caaaaagttc ggtatctgga tgaactgcag atttcctttt
     7081 cccaacaagt ttgtgtggga gatattcact atatttattc acgatatccc tgatatttta
     7141 ctgtatgctc cccaggtgac cacatggaga atcatgaaac tgttgcctga ttaccttcat
     7201 gcgcccggtt ttgatgtgat cagtaactac cttgttcaga aaactgatca cttgaaaaga
     7261 ctccaacttt ctgagcggat tgctgatgtt tttgacaaat atagcgtgta tcggcctgaa
     7321 atggtccttc gatgggataa ggggtcccat ggatcaggac agtctgagcg ctggcaggca
     7381 gaactgtgga gagggctttt caaaaatgga gaagaaaaac accgggcagg tattctgaga
     7441 gattttctca aagcgattac atatgcaaag gattcagtaa caggattacc caaacgcgtc
     7501 tccgtgttcg gcattcctgc tctgccccgg tatcattttg aggtcattca cgctcttgca
     7561 ttagtgacag atgtccatct gtttcttttg agccctacaa aagaatactg ggcagatatt
     7621 gtgccggaca aaaaaattgc aactcagcct gtgggtaaag atcttcattt tgaggtgggg
     7681 aatcaatttc ttgcttccac aggcaggctt ggacgggatt tcttttccat gttagtaagt
     7741 caaagctacg aagaatttcc atatttcaaa gatccggaag gagaatcact cctcgcgcgg
     7801 ctgcaatcag atat
//
LOCUS       DAOOMC010000127         7760 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdus sp. isolate AMR_MDS_2685
            NODE_11073_length_7760_cov_6.106814, whole genome shotgun sequence.
ACCESSION   DAOOMC010000127 DAOOMC010000000
VERSION     DAOOMC010000127.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864760
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdus sp. (wastewater metagenome)
  ORGANISM  Syntrophorhabdus sp.
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae; Syntrophorhabdus.
REFERENCE   1  (bases 1 to 7760)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7760)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,729
            Genes (coding)                    :: 2,708
            CDSs (with protein)               :: 2,708
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 7 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7760
                     /organism="Syntrophorhabdus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11073_length_7760_cov_6.106814"
                     /isolate="AMR_MDS_2685"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2699756"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..218
                     /locus_tag="PK125_11390"
     CDS             <1..218
                     /locus_tag="PK125_11390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB38747.1"
                     /translation="SHDQVLSGISHLLHCIVHNINFYVTVNHRLIQGFNEQKEALERY
                     LHLTALDLKLMTPKRFVTENLKSGQRA"
     gene            complement(406..1161)
                     /locus_tag="PK125_11395"
     CDS             complement(406..1161)
                     /locus_tag="PK125_11395"
                     /inference="COORDINATES: protein motif:HMM:NF024246.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HPB38748.1"
                     /translation="MKMKRKIIEIDESKCNGCGQCVEACAEGAIQLINGKAKLIADNY
                     CDGLAACVGECPVDAIKVIEREADAFDPEAVEHHLRQLSAQNSGFRTETSLPCGCPST
                     KMEMFNSHCDAANQPVSHACADSALTHWPVQIRLVPASAPFLQKAHLLVASDCTPVAY
                     PDFHKDFLKGRTVLVGCPKFDDTAEYIKKFAEIFRMADIQSVTILIMEVPCCSKMPMI
                     IQEGMALAGKNIPTEIVVVSGKGIIVKRDKLAA"
     gene            complement(1274..2902)
                     /gene="hcp"
                     /locus_tag="PK125_11400"
                     /gene_synonym="priS"
     CDS             complement(1274..2902)
                     /gene="hcp"
                     /locus_tag="PK125_11400"
                     /gene_synonym="priS"
                     /EC_number="1.7.99.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR01703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016661 - oxidoreductase activity, acting
                     on other nitrogenous compounds as donors [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HPB38749.1"
                     /translation="MFCYQCEQTAKGEACVKSGVCGKQPDVAALQDLLVHTLAELSHC
                     AVEGRKVGVHIDRTTNVFTVEALFSTLTNVDFDPARFVELINECVALRDALKDKVIAA
                     GGKVSFPDGSPTVPGKTLEGLVKQGEAVSLDAKQGEDPDIRSLKHTLLFGLKGVAAYA
                     DHAQILGQEDQKVYDFVYDAMNAMLTANMSLGDWLAMVLKCGEINLRAMELLDAANTG
                     AYGHPVPTSVPLGAKKGKAILVSGHDLKDLEELLKQTEGKDIFVYTHGEMLPCHGYPG
                     LKKYAHFYGHYGTAWQNQHKEFAAFPGAILMTTNCIQKPQDSYKDNIFTAGTVGWPGV
                     THITDRDFTPVINRALALPGFSADSNGRSVMVGFARETVMGVAGKVIDAVKAGQIKHF
                     FLVAGCDGAKPGRNYYTEFVEKLPKDTVVLTLACGKFRFFDKDLGDIGGIPRLLDIGQ
                     CNDAYSAIQVAVALSKAFNVGVNELPLSMILSWYEQKAVAILLTLLYLGIKNMRLGPS
                     LPAFVTPNVLKVLVENYGIKPITSPDEDIKAILG"
     gene            3244..3621
                     /locus_tag="PK125_11405"
     CDS             3244..3621
                     /locus_tag="PK125_11405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB38750.1"
                     /translation="MLKIIRFFIVPATASMLLLGFTLTAAIADWYNSCTPKHVAVFAV
                     KPYQRIHVECTEPAPGTDYIYFAYYGAKKFSPDSDRFLNLITFAMQNSKTLAIEFDPS
                     DQSGSKIGCNNSNCRLIQSLYVY"
     gene            complement(3913..5034)
                     /locus_tag="PK125_11410"
     CDS             complement(3913..5034)
                     /locus_tag="PK125_11410"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: protein motif:HMM:NF015047.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016703 - oxidoreductase activity, acting
                     on single donors with incorporation of molecular oxygen,
                     incorporation of one atom of oxygen (internal
                     monooxygenases or internal mixed function oxidases)
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HPB38751.1"
                     /translation="MHPDIHLPPLTIKGKVIRIPIIQGGMGVGVSLHPLASAVAKEGG
                     IGIVSSAALDRLVSKRTGKKLNTYEAVCEEISLARKNGGYAGINIMVALARDYDDSVR
                     GAIDAKADIIISGGGLPMNLPSIHPPDTTALVPIVSSPRALELICKKWERWRYRPDAV
                     VLEGPLAGGHLGFRFDELDLESNKLENLLPQVKDVAMKYGNFPVIAAGGIFDRSDILR
                     FLALGADGVQMGTRFLATEECSATPAYKEAVLSAGKDDILVAEKPGSPCGLPFRVIKQ
                     SPMFVSALQGRREPKCDKGYVLLKDRDGVFSKCSAKTGTQESFCICNGLLSSAGYNPD
                     IEEPLYTVGTNAWRLNKIVSVKDLMDELTGETTSAAVNY"
     gene            complement(5122..5361)
                     /gene="rpoZ"
                     /locus_tag="PK125_11415"
     CDS             complement(5122..5361)
                     /gene="rpoZ"
                     /locus_tag="PK125_11415"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019939244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HPB38752.1"
                     /translation="MARITVEDCMDYVENRFELVHLSAKRAKQLIKGTKPLTKSSNKE
                     IVTALREIAEELVYFEKKDVLDKKETPYQLSSFAP"
     gene            complement(5342..5962)
                     /gene="gmk"
                     /locus_tag="PK125_11420"
     CDS             complement(5342..5962)
                     /gene="gmk"
                     /locus_tag="PK125_11420"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: protein motif:HMM:NF012832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HPB38753.1"
                     /translation="MLNNEADSSQGSGVSKKKGHLFVVSGPSGVGKTTLIERFLKEDS
                     FSRFSISYTTRKQRASEVNGREYYFVDRDTFHKMITESHFLEWEEVHGQLYGTPQKEV
                     LHALQQGIDLFLDIDVNGALKVKERYRNACLIFVEPRSQDDLMKRLALRGEKEISLRM
                     KRVSQEMEKKYLFQYCIINDKLEVAYRDFFKIIEDVRREDNGKNHC"
     gene            complement(5952..6821)
                     /locus_tag="PK125_11425"
     CDS             complement(5952..6821)
                     /locus_tag="PK125_11425"
                     /inference="COORDINATES: protein motif:HMM:TIGR00255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HPB38754.1"
                     /translation="MPKSMTGFAKVEMEYADGRLYGEARALNNRYLEITIRLPKIDYA
                     SEQKLRELVRTYVKRGKVDITIKLDKPFNNAGTPRINEPVVKHYISIIESLKRDYGLK
                     GDISIENILAFKDIIAYEENNNLTEDILFVSFENLLKKLDEERSKEGLMISVDLLKRL
                     DEIDVNIQHIETRHPLTNKVYEKKLRDKMLEVTQCSSIDEVRVLQELAIYMERLDITE
                     EIVRLKGHIANFRETLGSSDSMGRKLDFIIQEMVRETNTIGSKSNDLSINERVVKIKV
                     EIEKIREQVQNVE"
     gene            complement(6838..7326)
                     /locus_tag="PK125_11430"
     CDS             complement(6838..7326)
                     /locus_tag="PK125_11430"
                     /inference="COORDINATES: protein motif:HMM:NF025743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4416 family protein"
                     /protein_id="HPB38755.1"
                     /translation="MPEMEKKLISFFGKILSRTVPVPFLHTGYYEKEMGPSLLRYFLL
                     FKPLRAREELPFIKLKTNEFEASTSAEGKRTINIDPGYITLEQVILATTKGYTHRVYL
                     GAGIFADLTLTFTNGTFKALPWTYPDYGSPEMISLLNHWRNEYKKDLRGESLYEEWPH
                     LK"
     gene            complement(7365..>7760)
                     /locus_tag="PK125_11435"
     CDS             complement(7365..>7760)
                     /locus_tag="PK125_11435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB38756.1"
                     /translation="RLRNDERVTSLENVNARYMSFDDIGKKFEIITIDVSFISLKKIL
                     PVVVPFMSTGACVISLIKPQFEVGRYEVGKGGIVKDEQKIRKVLNEIKDFGKSLGLVH
                     KNEVEAPREKEKKNREYFVLWELRAAQNQ"
BASE COUNT         2024 a         1813 c         1602 g         2321 t
ORIGIN      
        1 atagccatga ccaggtatta agcggcataa gccatctttt gcactgcatt gtgcacaaca
       61 tcaattttta tgtcacagtt aatcacaggc taatacaggg tttcaatgaa caaaaagaag
      121 cacttgagag atatcttcat cttacggccc tggaccttaa attaatgacc cccaagaggt
      181 ttgtaactga aaacctgaaa tcaggtcaaa gagcctgata acaatctctc agtttagtca
      241 tcactggtcg cgtcataacg ggactccaca gcctgaaatc ttcagatttc ttcacattgc
      301 actaatgact gcatgccccg ttcatctttt ttctgaatga aaacatggtc tgaggcgctt
      361 ggagcatgga gaataaaacg ggcctgccta atccgtgatt catggttatg ctgcaagctt
      421 atctcttttg actatgatgc cctttccact gacgacgaca atttctgtgg gaatgttttt
      481 accggccaga gccatgccct cctggatgat cataggcatc tttgagcagc aagggacctc
      541 catgatcaga atggtaacac tctgaatatc agccattcgg aaaatttcgg cgaatttctt
      601 tatgtattct gcagtgtcat caaactttgg acagccaacc agaacagtgc gtccttttag
      661 aaaatccttg tggaaatccg gataagctac gggggtgcaa tcagaagcta cgagaagatg
      721 ggctttttgg aggaatggag cagaagccgg tacaagccgg atctgaactg gccagtgggt
      781 aagggcggag tcagcgcatg catgggagac gggttgattt gccgcatcac agtgtgaatt
      841 gaacatttcc atttttgtcg agggacagcc acagggtagg gaggtttcag ttcggaaccc
      901 ggagttctgg gcactgagtt gtcggaggtg gtgttcgact gcctcggggt cgaaagcatc
      961 agcttcccgt tcaataacct taatggcatc gacaggacat tcgcccacgc aggcggcaag
     1021 accatcgcag taattgtcag cgatgagctt tgcttttcca tttataagct gtattgcacc
     1081 ctctgcgcac gcctctacac actgtccaca gccgttgcac ttgctttcat ctatttcgat
     1141 aatttttctc ttcatcttca ttgcgtccct tctccttttt tacttcgtga gctggcaacc
     1201 tttattagcc catagctcct ggtagtatcg gcatttatcc accaagaact atgagctatt
     1261 agttataaac tgtttagcct aatatggcct ttatgtcttc gtccggactg gtaattggct
     1321 tgatcccata gttttcaaca agcaccttca gaacgtttgg tgtaacgaat gctggcagcg
     1381 acggaccgag cctcatgttc ttgattccca gatagagaag agtcaacagg atcgccacag
     1441 ccttctgctc gtaccacgaa agaatcattg aaagaggcag ttcattgaca cctacattga
     1501 aagcctttga aagggcaact gccacctgaa ttgctgaata ggcatcatta cactgaccaa
     1561 tgtccagcaa cctgggaata ccaccaatat caccaagatc tttatcgaag aaacggaatt
     1621 tcccgcatgc aagggtaagt acaaccgtat cctttgggag tttttccaca aattctgtat
     1681 agtagtttcg acctggttta gcaccgtcac atcccgctac tagaaagaag tgcttgatct
     1741 gcccggcttt tactgcatca atgacttttc cggccacacc cataaccgtc tctctggcaa
     1801 acccgaccat aactgatctt ccgttggaat cagcggagaa gccaggaaga gcaagggctc
     1861 tgttgatgac aggagtgaaa tctctgtctg ttatatgtgt tactccgggc catcccactg
     1921 ttccggcagt aaagatgttg tccttatagc tgtcctgtgg tttctgtata cagtttgtgg
     1981 tcatcagtat tgcgccggga aaagcggcaa attctttgtg ctggttctgc caggctgttc
     2041 cataatgacc gtagaaatga gcatattttt tgagtccagg atagccatgg cacggaagca
     2101 tctcgccatg agtataaaca aatatatcct taccttcagt ctgcttgagc aattcctcga
     2161 gatcttttag gtcatgtcca gatacaagga ttgcttttcc tttttttgcc ccaagaggta
     2221 cagatgttgg tacaggatgc ccataagctc ctgtgtttgc agcatcaagc aattccattg
     2281 cccgcaggtt gatttcgccg cacttaagaa ccattgccag ccagtcacca agcgacatat
     2341 ttgcggtgag catagcattc atagcatcat acacaaaatc atagactttc tggtcttcct
     2401 gccctagtat ctgagcatgg tcagcatacg ctgccacacc ctttaatccg aagagcaggg
     2461 tgtgtttcaa ggaacgtatg tccggatcct ctccctgttt tgcatcaagg gatactgcct
     2521 ccccctgttt taccagacct tccagggttt tccctgggac tgttgggctg ccgtcaggaa
     2581 aagaaacctt tccgccagca gcaatgacct tgtccttcag ggcgtctctt aaagccacac
     2641 actcgttgat aagttctaca aacctggcag gatcaaaatc aacattcgtc agggttgaga
     2701 aaagcgcttc aacagtgaac acgtttgtag tgcgatcaat atggacgcca acctttcgtc
     2761 cctcaaccgc gcagtgggaa agctctgcca gggtgtggac aagaagatcc tgtaacgctg
     2821 caacgtcagg ctgtttccca catacaccgc ttttaacaca agcctcacct tttgctgttt
     2881 gctcacactg ataacaaaac atattctcct cctttttggt tttgttctga atcgtgaatg
     2941 aaatataatc atcttaaagg ccgatatgtt tgacttaaat caaaaaaaga agcatttttc
     3001 aaatttttta taatgatgaa cgggatatca tcttttcctt ctcacccgct cattaacacc
     3061 caaatcgtta agtgtttgag aagtttgcct gtacagtgtt ggcctgttga atgaccaata
     3121 aggaagagaa gcttgtaaaa gagttttcac tctgtgctga cactgtgcct gtacgtgaac
     3181 ctgtttgaca aaacctgata ctttaagtat agtaaccgaa acattcaatc taggaggttt
     3241 catatgttaa aaattatcag gtttttcatt gttccagcca ctgcatccat gcttttgtta
     3301 ggttttaccc tcactgcggc gattgctgac tggtataata gttgtacacc aaagcatgtt
     3361 gcagtttttg ctgtgaaacc ctatcagcgc attcacgttg aatgcacaga acctgctcca
     3421 ggtactgatt atatatattt tgcctattat ggagcaaaaa aattctctcc agactcagac
     3481 cggtttctca acttgattac ttttgcaatg cagaacagca aaactttagc aatagaattc
     3541 gatcctagtg atcagagcgg tagtaagatt ggatgcaata actctaattg cagacttatt
     3601 caaagtttat atgtgtatta aaaatgaact gatgaaatcg aataaatctt accttaaaaa
     3661 tcaaactaaa aatacagacc tggctgcagc cgggtctgta ttttattacg attatagacg
     3721 attggcgtga gagttactga ttctgatggt gtacgccttt tgtgagcggg caaaggtcat
     3781 tgttgaagcg gatgagggta tttgaggtga atgcagtgag ctgatggcca tcctcgctgg
     3841 aaacaacagg taaaaaatta agacgaaatt gtgttcaggt acgggtaatt ttgacaaaag
     3901 atttggattt ttttaataat ttacagcagc agatgtagtt tcgcctgtca gctcgtccat
     3961 caaatctttt accgacacta ttttattgag cctccacgca ttggtgccaa ctgtgtaaag
     4021 gggttcttct atatcaggat tataacctgc cgaactcaga agaccattgc atatacaaaa
     4081 actttcctgg gttcctgttt tggcggagca cttgctaaat accccatccc tgtctttgag
     4141 gaggacatac cccttgtcac attttggttc tctcctgccc tgaagagctg aaacgaacat
     4201 gggagattgt ttaatgaccc ggaagggcag accgcatggt gaacctggtt tttcagccac
     4261 cagaatatca tccttgccag cactcaaaac cgcctcctta taggctggag ttgcactgca
     4321 ctcctccgta gcgaggaacc gtgttcccat ctgcactcca tctgcgccca gggcgaggaa
     4381 tctgaggatg tcgctccgat caaagatccc acctgcagct atgacgggga aattgccgta
     4441 tttcatggcc acgtctttta cctgggggag caggttctcg agtttatttg attcaaggtc
     4501 caactcgtca aacctgaagc cgaggtggcc gccggctaat ggtccttcca gaacaacagc
     4561 gtcaggtctg tatctccacc gttcccattt cttgcatatc aactcgagtg ctctcggtga
     4621 tgaaacaatt ggtacgagag cagttgtatc cggagggtgg attgacggga gattcatcgg
     4681 caaacctcct ccagagatta ttatatccgc ttttgcatca atagcccccc gcaccgaatc
     4741 atcgtaatct ctggcaagcg caaccatgat attgattcct gcatacccac cgttttttcg
     4801 tgcaagggag atctcttcac aaacagcttc gtaggtgttc agtttttttc cagttctttt
     4861 ggagacgagc cgatcgaggg cggcgcttga cacaatgcct atacccccct ctttagctac
     4921 tgcgcttgcc aatgggtgga gagagactcc aacgcccata cctccctgga taatgggtat
     4981 cctaatgacc ttacctttga tggtaagcgg tggcaaatga atatcaggat gcattgaact
     5041 ccttttatcc gaacccttac cggggtgcga atgagcgatc tatcgttcgt ttcgagcagg
     5101 aaagaatgat aaatgggtta gttatggcgc gaagctggac agttggtatg gcgtctcctt
     5161 tttatccaga acatctttct tttcaaaata tacaagctct tcagcaatct ctcttaatgc
     5221 agtgacaatt tccttgttcg aggattttgt gagtggtttt gttcctttaa tcaactgctt
     5281 ggccctcttt gcggaaagat ggacaagctc aaagcggttc tctacataat ccatacaatc
     5341 ttcaacagtg attcttgcca ttatcctccc tcctcacatc ctcaatgatt ttaaagaaat
     5401 cacgataagc cacttcaagt ttatcattaa tgatacaata ttggaagaga tattttttct
     5461 ccatctcttg gctcaccctc ttcatcctca aagaaatctc tttttcaccc ctcagggcaa
     5521 gtcgcttcat gagatcatcc tgagagcggg gttccacaaa aataagacaa gcattcctgt
     5581 aacgctcttt gaccttgagg gcaccattta catcaatatc cagaaaaaga tcgataccct
     5641 gttgcaaggc atggagaact tctttctgtg gagtgccgta caattggccg tgaacctcct
     5701 cccattcaag aaagtggctt tctgtaatca ttttatgaaa ggtatccctg tcgacgaaat
     5761 agtattccct tccattcact tcacttgctc tttgttttcg agttgtgtat gagatggaga
     5821 atctgctgaa agaatcttct ttaagaaatc gctcaatgag cgttgtcttt cctacacctg
     5881 aaggcccaga cacaacaaac aaatgtccct tctttttgct gacccctgac ccctgactgc
     5941 tgtcagcttc attattcaac attctgtacc tgttccctga ttttttcaat ttcaaccttt
     6001 atcttaacaa cccgttcatt gatagaaaga tcattcgatt tgctcccgat tgtgttggtt
     6061 tcccgaacca tctcctggat gatgaaatcg agttttcttc ccatcgaatc agatgagccg
     6121 agggtttccc tgaagtttgc aatatggcct tttagcctga caatctcttc ggttatgtcg
     6181 agccgctcca tatatatggc aagctcctga agaaccctga cttcatcaat ggaagaacat
     6241 tgagtaactt caagcatctt atcccgtagt tttttttcgt agactttatt tgttagaggg
     6301 tgtcttgttt cgatgtgctg aatattaacg tcaatttcat ccaatctctt gagaaggtca
     6361 acactgatca taagaccttc tttggatctt tcctcgtcaa gtttttttaa cagattttcg
     6421 aaggaaacga agagtatatc ttcagtaaga ttgttattct cttcgtaggc tataatgtcc
     6481 ttaaaggcca atatgttttc tatggagata tcacccttga ggccataatc ccgctttaag
     6541 gactctataa ttgaaatgta atgttttacg actggttcat tgatcctggg ggtacctgca
     6601 ttattgaaag gtttgtccag ttttatcgta atatctactt tacctctctt cacgtacgtt
     6661 ctgaccagct ctcttagctt ttgttcgctt gcatagtcta tcttcggaag ccttatggtg
     6721 atctcaagat aacggttatt taaagccctt gcttcaccgt aaagccttcc atcagcgtat
     6781 tccatctcta cttttgcaaa acctgtcatg ctttttggca tagcaacacc cttcttctta
     6841 tttcaagtga ggccattcct catacaagga ttcacctctt agatcttttt tatactcgtt
     6901 tcgccagtgg tttaacaggg atatcatttc aggactgcca tagtctggat aggtccatgg
     6961 gagagcttta aaggtcccat ttgtaaatgt aagggtaaga tcagcaaaaa tccccgcgcc
     7021 aagataaaca cgatgggtat agcctttggt cgttgccagt attacttgtt ccagtgtgat
     7081 atatccagga tcaatattga tggtacgctt gccttctgct gatgtgctgg cctcaaattc
     7141 atttgttttt agttttatga agggaagctc ttctctggct ctcaaaggtt taaacagaag
     7201 aaaataccgc aagagactgg ggcccatctc cttctcataa taacctgtgt gtaaaaaagg
     7261 cactggcact gttcttgaca gaattttccc gaaaaaagaa ataagctttt tttccatttc
     7321 aggcattagc ccgttttcct gatatataat acttacaaag aaccttactg gttttgggct
     7381 gcccggagtt cccatagaac aaaatattcc cgattctttt tttccttttc ccggggtgct
     7441 tctacttcgt ttttgtggac taaacccagg ctcttaccga agtcctttat ttcgtttaaa
     7501 accttccgaa ttttctgttc gtccttaaca atgccgcctt tacccacctc gtatctccca
     7561 acctcgaact gtggttttat aagggagatg acgcatgcac cggtactcat aaagggtact
     7621 acaaccggca aaattttctt gagcgatata aaagatacat caatagtaat aatctcgaat
     7681 tttttaccaa tatcatcaaa ggacatatac cgcgcattaa cattctcaag tgaagtaacc
     7741 cgttcatcat tgcgcagacg
//
LOCUS       DAOOMC010000128         7754 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdus sp. isolate AMR_MDS_2685
            NODE_11080_length_7754_cov_5.810365, whole genome shotgun sequence.
ACCESSION   DAOOMC010000128 DAOOMC010000000
VERSION     DAOOMC010000128.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864760
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdus sp. (wastewater metagenome)
  ORGANISM  Syntrophorhabdus sp.
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae; Syntrophorhabdus.
REFERENCE   1  (bases 1 to 7754)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7754)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,729
            Genes (coding)                    :: 2,708
            CDSs (with protein)               :: 2,708
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 7 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7754
                     /organism="Syntrophorhabdus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11080_length_7754_cov_5.810365"
                     /isolate="AMR_MDS_2685"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2699756"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..187)
                     /locus_tag="PK125_11440"
     CDS             complement(<1..187)
                     /locus_tag="PK125_11440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB38757.1"
                     /translation="MSSNRSMYSAPFADSPGSFLAITCLLCEYDMPFSSTFLQRTKID
                     IIPPEFLEQVVQWNSHEI"
     gene            381..923
                     /locus_tag="PK125_11445"
     CDS             381..923
                     /locus_tag="PK125_11445"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HPB38758.1"
                     /translation="MIKDWDLIDSKVDRDYRVFRINVETSVSPRTKKAGRFYVIDTND
                     WVNIIPITEDGQVVMIQQYRHGSKNITLEIPGGLVDDESPENAALRELLEETGYQGDD
                     VTYLGATNPNPAIFNNLCHTFLVRNVKKVSETNLDDNEDIEVTLKPIEEIPSLIADGS
                     INHALVIIAFHFYFSRQSQF"
     gene            929..1459
                     /gene="mobB"
                     /locus_tag="PK125_11450"
     CDS             929..1459
                     /gene="mobB"
                     /locus_tag="PK125_11450"
                     /inference="COORDINATES: protein motif:HMM:NF015181.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein B"
                     /protein_id="HPB38759.1"
                     /translation="MVKESIPYLAFVGRSKTGKTTVIEKLIPALREKGFKIAVIKHHH
                     HDFDIDIPGKDTYRYKQAGASTSIIASPGKVAVVEDTARELPLHEIITRYVHDDVDLL
                     LIEGFKKENIPKIEVFRQKEDGTTPVCTGDKNLIAVVTDESVKTSLPIFTRNDVQGIA
                     EFIVNRFLRQKSSGVP"
     gene            complement(1413..2672)
                     /gene="lpxC"
                     /locus_tag="PK125_11455"
     CDS             complement(1413..2672)
                     /gene="lpxC"
                     /locus_tag="PK125_11455"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: protein motif:HMM:NF015299.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HPB38760.1"
                     /translation="MNKVLIIDDEKQILDSLSSILRDEGFQVFTAREGREGLELFDTA
                     RPEIVLLDIWMPGLDGLQVLKMIKEKEKEAIVIVISGHGTISTAVEAVKMGAYDFLEK
                     PLSIDKVLEVISRGLAGENQKTEVAEDVKVGISKGPDVYKQKTIGESIVVYGVGLHSG
                     VKTGMILLPMPEGTGIVFEHIPDGERIPAHIDYVFSVGYASTVKGKNCQVRTIEHLMA
                     TCHMFGITNLLVKVSEEVPIFDGSALEMCNNIEKAGIVEQKEGVEPLKIDTKIVLSNP
                     GDGRHLSIEPSKRFEVEYILEHPMPIGRQSYHFAGNRDDFLQNIAPARTFGFIKDFER
                     LAKMGLGSGGRVNNVILLNDEEVVNTTLRFPDEFVRHKVLDLIGDIYLLNRPVIGRIS
                     AKQTGHLENIALVKELQKIFVAKNGLQ"
     gene            2803..3804
                     /locus_tag="PK125_11460"
     CDS             2803..3804
                     /locus_tag="PK125_11460"
                     /inference="COORDINATES: protein motif:HMM:NF014605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HPB38761.1"
                     /translation="MEEKTEEGYLRLSFDDTNVARNLFGPRDENIKYLKKYFNIRTSI
                     RGNHLTIMGNKKDIDNTNRVINELQGIVRKGLAINQADIDHAVRYLSHTKGDMEELHK
                     DQIYIMPSRKLIAPKSKNQKRYLDAIKKYDMVIGIGPAGTGKTYLAMAMALSSYFRKE
                     VSRIILTRPAIEAGEKLGYLPGTMYEKVNPYLRPLHDALFDMVDMDRATRLIEKGVIE
                     IAPLAFMRGRTLNDAFVILDEAQNTASEQMKMFLTRLGFSSKTVITGDITQIDLPDKK
                     TSGLVEIQAVLKGVKGIRFVYFSEKDVVRHPLVQKIIRAYESRKAVLEDIKNAERKS"
     gene            3785..5491
                     /locus_tag="PK125_11465"
     CDS             3785..5491
                     /locus_tag="PK125_11465"
                     /inference="COORDINATES: protein motif:HMM:NF019318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HPB38762.1"
                     /translation="MLSEKAKPPSYLNYRRLLVIFFLSLLLSIVCSTKDPFKLSEYQY
                     GDIAKKNIKSPADVYLPDKDTTLKKGEIIVREGDRITDEHLQKLSRIEVRESTAAFSV
                     KNLVLIFLLLFFSLTLIYEYADKNIKKFTLTEKDLNFAAIFTSFMALLVKASTLIFEH
                     FAPGAAPYLLYIIPIFCYGIILRIVLFSEAAIIFSILFSIVMGILFENSLPIFLYTLL
                     GNILASYYSGKCENRNTVLKAGLYTAFIMSLIVILFDSLQGTSPSNIPLKMAFIIVNG
                     VGSSFIALGLLPVIEHIFNYTTDIKLLELANLEHPLLEEMMVNAPGTHHHSIVIGNLA
                     KAAAESIGAHPLLARVSAYYHDIGKLKMPHYFIENRVGNDDAHRGLTPNMSALIILSH
                     VKEGIEMARQYRLGKKITDMIGQHHGTSLVTYFYNRAKELEDPRMHVTEEKDFRYAGP
                     KPQTKEAGIIMLADSVEASSRLLDDPTPKRIETHVQEVIEKIFLDGQLDECELTLKDL
                     HAIQKSFIAILIGIFHHRIEYPERTLHEGTDKRVTKVSGNGQKNGQKDNRKFTSIFKA
                     TG"
     gene            5388..5843
                     /gene="ybeY"
                     /locus_tag="PK125_11470"
     CDS             5388..5843
                     /gene="ybeY"
                     /locus_tag="PK125_11470"
                     /inference="COORDINATES: protein motif:HMM:TIGR00043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HPB38763.1"
                     /translation="MKVLIRESPKFLGTDKRTVRKIIENLLASLKLPDRDLSILFVDN
                     KKIRQLNKQYFHKDYPTNVISFSYMDGLTSEVIGDIVISIERTREEAESLSVPFYERL
                     FTLIIHGLVHILGYDHTGDKNQARRMRYREKKLLNFIISHSLYQKLICR"
     gene            5847..7382
                     /gene="lnt"
                     /locus_tag="PK125_11475"
     CDS             5847..7382
                     /gene="lnt"
                     /locus_tag="PK125_11475"
                     /inference="COORDINATES: protein motif:HMM:TIGR00546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006497 - protein lipidation [Evidence
                     IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HPB38764.1"
                     /translation="MRFFDFFHSNARSLNFSGQIGLPVLSGILLIVCQPPVSLFPFAF
                     IALIPLLYAMAKYPYRHPFLPGFITGIVAYLGLIYWVIVAMNTYGGIDIFTSTLILLL
                     FVLYLSLYLGCFTTAVFILEKKMSVPVYLSVPIVWTLLEYLRGVALTGFPWSFLGHSQ
                     HNFLIFIQIASITGTYFISFLIAAINCIVFFVALKKPISKIYLTVILVIVTASVAFGF
                     IRVNEPQDAKLTAAIVQGNIRQDVKWDDNFKIKTIQIYSENTIRKGNNVDFVIWPETA
                     LPLIFNEETYVNQYIKTLPSMVNSHLLFGTIWKDQRGKVYNSAYVIGKNGDVNGIYNK
                     AHLVPFGEYTPLVRYLPFLEKITAQGAGFASGNGHDPVITPSGKVGILICYEGVFPYI
                     TNATVARGAQFLVNLTNDAWYEKTSAAFQHLAFYVFRAVETDRYILRAANTGISAIID
                     PRGRITARTPIFEEQVLKGNFSLRDSMTSYIVYGDYFILILFLSLLTIITVQFIRRNK
                     KQR"
     gene            complement(7536..>7754)
                     /locus_tag="PK125_11480"
     CDS             complement(7536..>7754)
                     /locus_tag="PK125_11480"
                     /inference="COORDINATES: protein motif:HMM:NF018513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HPB38765.1"
                     /translation="IHYGIIMIVNIECGFLTPPFGLNLFVAMAIMKRSLVEIGKSILP
                     FILLFLGCLVIITYVPQLSTLLPKLLLR"
BASE COUNT         2271 a         1774 c         1560 g         2149 t
ORIGIN      
        1 aaatctcgtg tgagttccac tgaacaacct gctcaaggaa ttctggtgga atgatatcta
       61 tctttgttct ctgcaggaaa gttgaagaaa aaggcatgtc atattcgcat aagaggcacg
      121 ttattgccag aaaagaaccc ggtgagtcgg caaatggagc actgtacatg ctcctgtttg
      181 atgacatttg actctatctc tcttgtttga caacttggca cagaaattct gacattcttc
      241 agcaccaaaa ataaggatgt cttaggtgac tgagaaacgc gtatgagcct ttaatggttt
      301 aacaaacatc attgtttttt caaaaacaac cataggtttg cattatcttc actggctatg
      361 gtatttttaa tgaaaaggcc atgataaaag actgggacct catcgattca aaggtagacc
      421 gagattacag ggtgttcaga attaatgtgg aaacatctgt gtctccaaga accaagaaag
      481 ccgggcggtt ttacgtcatt gacaccaatg actgggttaa tatcatcccc atcaccgagg
      541 atgggcaggt ggtaatgatc cagcagtaca ggcatggatc aaaaaatatc acccttgaaa
      601 taccaggcgg gcttgtagat gatgaaagtc cggaaaatgc agccctgcgg gaactccttg
      661 aagagacagg ctatcaggga gatgacgtca catatttggg tgcaacgaat ccaaacccag
      721 ccatttttaa caacctctgc catacctttc tcgtaagaaa cgtgaaaaaa gtgtccgaaa
      781 caaaccttga cgacaatgag gatatcgagg taaccctgaa acccattgaa gaaatcccga
      841 gcttgatagc tgacgggagc ataaatcatg ctctcgtcat catagcattt catttttact
      901 tttcccggca atcacaattt taaaccccat ggtaaaagag agcatacctt accttgcctt
      961 cgtcggtaga tcaaagactg gaaaaactac tgtcattgaa aagcttatcc ctgccctccg
     1021 tgaaaaaggg tttaagatag cggttatcaa acatcatcac catgatttcg atattgatat
     1081 accgggaaaa gatacatacc gttataagca ggccggtgcc tctacaagca ttattgcctc
     1141 acccggtaaa gtagcggttg ttgaagatac tgcgagagaa ctcccccttc atgagattat
     1201 taccaggtat gtacatgatg atgttgacct cctgctcata gagggcttca agaaagaaaa
     1261 cattcctaag attgaggtat tccgtcaaaa agaagatggg accacaccag tgtgcaccgg
     1321 agataaaaac cttattgccg ttgttactga tgaatcagtg aaaacgtctc ttcccatctt
     1381 cacacggaat gatgttcagg ggattgccga gtttattgta aaccgttttt tgcgacaaaa
     1441 atcttctgga gttccttgac cagagcaata ttctcaagat gacctgtttg ttttgcagag
     1501 attctgccta taacgggcct gttcaagaga tatatatcac cgataagatc aagcaccttg
     1561 tgcctaacaa actcatcagg aaaccgcaat gtggtgttaa caacctcttc atcattcaga
     1621 aggattacat tgttaacccg cccgccggaa ccgagtccca tctttgcaag gcgttcaaaa
     1681 tccttgataa atccgaaggt tcttgcaggg gcaatattct gtaaaaaatc atctctgttg
     1741 cctgcaaagt ggtatgactg ccggccgatc ggcatgggat gttccaagat atattcaacc
     1801 tcgaaccttt ttgagggctc gatagacagg tgacgaccgt caccagggtt ggaaagaaca
     1861 atttttgtgt ctattttgag aggctctacc ccttcctttt gttcaacaat tcctgctttt
     1921 tcaatattat tacacatctc aagggctgag ccatcaaata tgggcacctc ttcgcttacc
     1981 tttaccagga gattggtgat tccgaacata tggcacgtgg ccataaggtg ctctattgtg
     2041 cgaacctggc agttctttcc tttaacagta gatgcatatc ccaccgagaa cacatagtcg
     2101 atgtgtgcag ggattcgttc accatcaggt atatgttcaa aaacaatccc ggtgccttct
     2161 ggcatgggca ggagaatcat gccggtcttg acccccgaat gcaggccaac cccgtacaca
     2221 acgatacttt ctccaatagt cttttgtttg taaacatcag ggccttttga aataccaacc
     2281 tttacgtctt cggcaacttc tgttttttgg ttttcaccgg caagccctcg tgaaatcact
     2341 tccaacacct tatcaatgga taggggcttt tcaagaaaat cgtatgcacc catttttacg
     2401 gcctcaactg ctgtcgagat tgtcccatgt cctgatatga caattacaat ggcctctttt
     2461 tctttctctt tgatcatttt gagcacctga agaccatcaa gtcctggcat ccagatatca
     2521 agaaggacaa tttcaggcct ggctgtatcg aagagttcta gtccctccct gccttccctg
     2581 gctgtaaaaa cttgaaatcc ctcatccctc aagatggagg aaagactatc gagaatttgc
     2641 ttttcgtcgt caataatgag aaccttgttc atagaggata ttttacctga aagcactctt
     2701 atttacaaat ttacaggatt cataccctta tagtgcaaat atcttttttg tgctatacta
     2761 tgttcacaaa gttaaggaac ttggaggtca acaacatact aattggaaga aaaaactgaa
     2821 gaaggttacc tgaggttatc ctttgacgat accaacgtag ctcgaaacct ttttggccca
     2881 cgcgacgaaa acataaaata cctgaaaaaa tacttcaata tcagaacaag cattcgtggg
     2941 aaccacctta ccatcatggg caacaaaaaa gatatcgaca atacaaacag agtgatcaac
     3001 gaactccagg gaattgttcg aaaaggcctt gccatcaatc aggccgatat tgaccacgct
     3061 gttagatacc tttcccatac caagggcgat atggaggaac tccacaaaga ccagatttac
     3121 ataatgccct caaggaagct tattgcacca aaatcaaaaa accaaaagcg ctatcttgat
     3181 gccatcaaaa aatatgacat ggtcattggc atcggccctg ccggtacagg aaagacctac
     3241 ctcgccatgg caatggccct ttcatcgtat ttcagaaagg aggtttcacg cattattctc
     3301 acaaggccgg caatagaagc cggtgagaaa ctcggatatc ttcccgggac catgtatgaa
     3361 aaggtgaatc cctacctacg acctcttcat gacgctcttt tcgacatggt agacatggat
     3421 agagcaaccc ggcttattga aaaaggtgta attgaaatcg cccctctggc atttatgcgt
     3481 ggccgcacac ttaacgacgc ctttgtaatt cttgatgaag cacaaaatac agcctcggaa
     3541 cagatgaaaa tgtttctcac gaggctcggg ttctcttcga agaccgtcat aaccggcgat
     3601 attacccaga ttgatctacc tgacaaaaaa acgagcggcc ttgttgagat tcaagcggtg
     3661 ttaaaaggtg tcaaaggcat ccgatttgtt tacttttcag agaaagatgt ggtaagacat
     3721 ccgcttgtcc agaagatcat tcgggcgtat gaatcccgga aggctgtcct ggaggatata
     3781 aaaaatgctg agcgaaaaag ctaaacctcc ctcgtacctg aattatcgca ggcttcttgt
     3841 catttttttt ctctccctcc tcctatcaat agtatgcagc accaaagatc cctttaagtt
     3901 gtcagaatat cagtatgggg atattgctaa gaagaatata aaatcacctg ctgatgttta
     3961 ccttccagac aaagacacca ccttgaagaa gggagagatt attgtcagag aaggtgacag
     4021 aattactgac gaacatcttc aaaaactctc ccggatagag gtcagggaaa gtactgcggc
     4081 tttctctgta aaaaacctgg ttctgatctt tcttctcctt ttcttttctc ttaccctcat
     4141 atacgaatac gcagataaga atataaagaa atttaccctt acagaaaaag acctgaactt
     4201 tgcagctatc ttcacctctt tcatggcact tcttgtgaag gcttccacgt tgatttttga
     4261 acattttgca cccggtgctg ccccttatct tctctatatt attcccatat tctgttatgg
     4321 catcatactg aggatagtcc tgttctctga agcggccatt atattttcca tactcttctc
     4381 gatcgtcatg ggcatcttgt ttgagaatag ccttccaatc tttctttata cacttctagg
     4441 taatatcctt gcatcttact attctgggaa gtgcgaaaac agaaacacgg ttcttaaagc
     4501 cggtctctat acagctttta tcatgtctct tattgttatt ctttttgact ctcttcaggg
     4561 aacctcaccc tcaaacatcc ccctgaagat ggcatttatt attgttaacg gcgtaggcag
     4621 cagctttata gcactgggcc ttcttcctgt catagaacac atttttaatt acaccacgga
     4681 catcaagctc cttgagctgg caaacctgga acatccactc cttgaagaga tgatggtaaa
     4741 tgcgcctgga acgcatcacc acagtatagt tatcgggaat cttgcaaagg ctgcagctga
     4801 aagtattggt gcccatccac ttctagcgag ggtatccgcg tattaccatg acatcggcaa
     4861 actaaaaatg cctcactatt ttatcgagaa ccgggtgggt aacgatgatg cccacagggg
     4921 actcacccct aacatgagcg cgctgattat cctttcccac gtcaaagaag ggattgaaat
     4981 ggcacggcag tacagacttg gaaaaaagat aaccgatatg attggtcagc accatggcac
     5041 aagccttgtc acatattttt ataaccgtgc gaaagaactt gaagacccaa ggatgcatgt
     5101 gactgaggaa aaagatttca gatatgccgg gcccaaacct caaacaaaag aggcaggcat
     5161 catcatgctg gcagactctg ttgaagcgtc atcacgcctt ctcgacgatc caacaccaaa
     5221 aagaatcgaa acacacgtcc aggaagtcat tgaaaaaata tttctcgacg ggcagcttga
     5281 cgagtgtgaa ctaaccctca aagacctcca cgccattcag aaaagtttca ttgctattct
     5341 cattggtata tttcatcaca ggattgaata tccagaaagg acccttcatg aaggtactga
     5401 taagagagtc accaaagttt ctgggaacgg acaaaagaac ggtcagaaag ataatcgaaa
     5461 atttactagc atctttaaag ctaccggata gggacctcag tatcctcttt gtggataata
     5521 aaaaaataag acaactgaat aaacaatatt ttcataaaga ctaccctaca aatgttatat
     5581 ctttttccta tatggatggg ctgacttctg aagtaatagg tgatattgta atctctattg
     5641 aaaggacccg cgaagaagcg gaatctctgt cagtcccttt ttatgaaagg ctttttaccc
     5701 tcattataca cgggttggtt catattcttg gctacgacca tacaggagac aaaaaccagg
     5761 cgaggagaat gcgctatcgt gaaaagaagc tcctcaattt catcatctcc cattctctgt
     5821 atcaaaaatt aatatgtagg tagcttatga gattttttga tttttttcat tctaatgccc
     5881 gaagtctgaa cttctccggg caaatagggt tgcctgtgct cagcggaatt cttctcattg
     5941 tatgccaacc cccggtctcc ctgtttcctt ttgccttcat agcattgatc cccctgcttt
     6001 atgctatggc aaaatatcca taccgtcatc ctttccttcc cggttttatc acaggaattg
     6061 ttgcatatct tggccttatt tattgggtta ttgttgcaat gaacacctat gggggtatcg
     6121 atatcttcac cagcacgctt atactgttgt tgtttgttct atatctttcc ctgtacctgg
     6181 gttgctttac gacagcagtt tttatacttg aaaaaaagat gtcagttcct gtttatctat
     6241 ctgtgcccat tgtgtggaca cttctggagt acctcagggg cgttgccctc acaggctttc
     6301 cgtggagctt ccttggccac tcccagcaca acttcctcat ctttattcaa attgcgtcta
     6361 ttaccggcac ttacttcata tcttttctca ttgccgctat aaattgcatc gttttctttg
     6421 tggcactcaa gaaaccaatt tcaaagatct atttaacagt gatactcgtc atagtaacag
     6481 cttctgttgc ttttggattt attagagtca atgagccaca agatgcaaag ttaaccgctg
     6541 ctattgtcca gggaaacatc aggcaagacg tcaaatggga tgataatttc aagattaaaa
     6601 ccattcagat atattccgaa aacacaatca gaaaaggcaa caatgtggat ttcgtaatct
     6661 ggcctgagac tgccctacct cttatcttta acgaagaaac gtacgtcaat caatacatca
     6721 aaacattgcc ctctatggtg aattcacatc ttcttttcgg cacaatctgg aaagaccaga
     6781 gaggaaaagt atacaattca gcctatgtca ttggaaaaaa tggtgatgta aacggtattt
     6841 acaataaagc gcacctcgtc ccattcggtg agtatacccc gttagtccgc tacctcccct
     6901 ttcttgagaa aatcactgcc cagggtgcag gttttgcttc cggcaatggc catgaccctg
     6961 taataacccc ctcgggcaaa gtagggattc ttatctgtta cgaaggtgtc ttcccctata
     7021 tcacaaatgc aacagtggca agaggggccc agtttctcgt aaacctgaca aatgatgcat
     7081 ggtatgaaaa aacctcagcg gctttccaac acttggcctt ctatgtcttc cgggctgttg
     7141 aaaccgacag atatattctt cgtgcagcga atacaggcat aagcgccatc atcgacccaa
     7201 gagggcgcat cacagccaga acgcctattt tcgaagaaca ggttttgaaa ggaaatttct
     7261 cactcaggga cagcatgacc tcatacatcg tatatggtga ttacttcata cttattttat
     7321 tcctttctct gctgaccatc ataactgttc aattcattcg aaggaacaaa aaacaaagat
     7381 aaagtaagtt ggtttgcccc gggacaggta tccgcgaaga aaaattatcc tttacagaca
     7441 ctttcattcg ctccacaacc ctcacccacc ccagttcgtg caaagataag catcaacatt
     7501 gcaggagagt ctgttttgga ttgggtcttg ttgtgttacc tcaggagtag cttaggaaga
     7561 agggttgaaa gttgtggaac atacgtgata atcacgaggc acccaaggaa caggaggata
     7621 aaaggaagga ttgatttgcc gatctctaca agggaccgtt tcatgatagc cattgcaaca
     7681 aacaggttca ggccaaaggg cggcgtcagg aaaccacatt ctatgttgac aatcatgatg
     7741 ataccgtaat ggat
//
LOCUS       DAOOMC010000129         7738 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdus sp. isolate AMR_MDS_2685
            NODE_11112_length_7738_cov_6.601978, whole genome shotgun sequence.
ACCESSION   DAOOMC010000129 DAOOMC010000000
VERSION     DAOOMC010000129.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864760
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdus sp. (wastewater metagenome)
  ORGANISM  Syntrophorhabdus sp.
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae; Syntrophorhabdus.
REFERENCE   1  (bases 1 to 7738)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7738)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,729
            Genes (coding)                    :: 2,708
            CDSs (with protein)               :: 2,708
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 7 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7738
                     /organism="Syntrophorhabdus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11112_length_7738_cov_6.601978"
                     /isolate="AMR_MDS_2685"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2699756"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2269
                     /locus_tag="PK125_11485"
     CDS             <1..2269
                     /locus_tag="PK125_11485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB38766.1"
                     /translation="DWFISLNHNPFPWYTYGGVDLSNDDRVGLFVEDHYYTRTDAAVV
                     FKRIDRWTGNEQYIYHGNDGTSMPWNDTAQLNYLNPEMREAMIQTILHVAKKFPVIRF
                     DAAMTLTKRHFQRLWFPQPGTGGAIPTRAEFGMSKEEFDRAMPNEFWREVVDRLAIEA
                     PDTLLLAEAFWLLEGYFVRTLGMHRVYNSAFMNMLRDEDNAKYRMVLKNTLEFDPEVL
                     RRFVNFMNNPDERTAVDQFGKDNKYFGICTLMVTLPGLPMFGHGQIEGYTEKYGMEYR
                     RAYWNEHPDQALIERHEREIFPLLHKRHLFAGVESFLLYDFFNLDGKVNEDVFAYSNS
                     FGPEKSLVAYHNKNSHASGWIRTSVMYSARNLEDGTRILMQKTLSVGLAIPPSGNRFT
                     VFRDHQSGLEYIRHNNDLCEKGLYIELGPYEYHVFIDFREYSDDEHHHYARLTDCLNG
                     KGVPNIDEALREIFLQPIHHSFSQLSNPGFLRYLWSIRGTLMNKQIDSIPPDVLSDIR
                     VRFGNLFSEVMHHTKGNGKIEELATNTSSMINCLITGIDLQANVPQDIKDNFLQNIFD
                     ITENLKDDDLVFYTLCHWTFVHALGAIVFETGRDARELSRSWIDEWFLGRFISQTLID
                     LGFNRETVSREVAVVKLLTSYQDWYKDFTNTYDLFSNLLRDNEVRDFLQINRHLDILW
                     FNKEAFEGLIFWMILVAVVKISCEAIKEENKEALARKAVCLMEPLYTALENSQYQVEK
                     LLGLLRTYSSSQKSE"
     gene            complement(2242..3120)
                     /locus_tag="PK125_11490"
     CDS             complement(2242..3120)
                     /locus_tag="PK125_11490"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: protein motif:HMM:NF013007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HPB38767.1"
                     /translation="MILIGENLNVSSKKVGEALKNKDRKIIREIAEAQAKAGMNMLDI
                     NIGPAPKIGSEMMSWVVRAVREVTDLPLSLDTTNIKAIETGLQVHEGKALINSISAQP
                     ERMAALLPLAKKYNAAFVGLTLGDEGFPRDANERGFFAAQIIAEAFQYNIPEEDIWLD
                     PIAPPIKTQQLQIQGCTEFALMIREAFPECKSIYGGSNISNGVPRNLRGMLNRTYLIM
                     LKRYGIYSAIVDAFDSELTNIAEGRRPELEKLVYSVMDGDDIDNSVLSEEEIKYVKTT
                     RVLLGQNLYSDFWLEL"
     gene            complement(3274..4611)
                     /gene="acsC"
                     /locus_tag="PK125_11495"
     CDS             complement(3274..4611)
                     /gene="acsC"
                     /locus_tag="PK125_11495"
                     /inference="COORDINATES: protein motif:HMM:NF003195.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA decarbonylase/synthase complex
                     subunit gamma"
                     /protein_id="HPB38768.1"
                     /translation="MALTGIQIFKLLPKTNCGECKYPTCLAFAMALASSKAEIDACPY
                     ITDEGRLQLSEASAPPIRQVSIGSSDYEIKIGGETVLFRHEKTFLNQPGFAILISDTM
                     DDAEIESRFEKVNFCQYDRIGTTLRPELVALKATGNDERFINLVKKAIEKPYGIVLIC
                     PIISLMKKAVEICKARKPLIYAATTENVQELGFLAKAFNCPLAVKGNGIEEVMHLTEQ
                     LTAMGVKDLVLDTSSRGVRESFQDQIIIRRAALVGKNKQLGFPTITFPCEMTDSLMKE
                     TLIASCFVAKYAGIIVLSDFKGESIFPLLLQRLNIYTDPQRPMTTEQGIYPINNPDSA
                     SPVLITCNFSLTYFIVSGEIENSRIPSWLCVMDTEGLSVLTAWAAGKFLGDGVGSFIK
                     KCGIIEKIEHKKLIIPGYAASILGEVEEELPGWEIQVGPREAVHIPAYLKMWK"
     gene            complement(4683..6890)
                     /gene="acsB"
                     /locus_tag="PK125_11500"
     CDS             complement(4683..6890)
                     /gene="acsB"
                     /locus_tag="PK125_11500"
                     /EC_number="2.3.1.169"
                     /inference="COORDINATES: protein motif:HMM:NF007078.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA decarbonylase/synthase complex
                     subunit alpha/beta"
                     /protein_id="HPB38769.1"
                     /translation="MSKIIASAAIRGAHKIYNKTEERYKDALERFGPDQEVGFPNTAY
                     YLPIIYAITGIGIKRIGDMRKVLDICKRLIPPPVREISSLPYLAPALDAGMATFFAEE
                     ILEAIKYLETPDFYAANSEDLTESSNWLGAANDVIFRKRGVEFVDGTAPGFAAIVGAA
                     PDSKTAAKIALELQEKNLYVFMCGENDGKRISEQLAETGVQIGWPTRLVPFGPDIYQA
                     VFAIGFACRVAMAFGGIRPGDFRRNLIYNKDRTYAFVLALGNVTDEWYANAAGAINWG
                     FPTIADTPIPQVLPTGICTYEHVVSNIPHHQIVQKAVEVRGLKVHVARVPIPVSYGPA
                     FEGERVRGEDIYLEMGGGRTIAVEWTITKKMEEVEDGRVEVIGPNIDQFHVGSQAHFA
                     LVAEVAGRNFQEDFEPILERQNHHLINQAQGIMHIGQRDIAWIRISKQAVEKGFKLQH
                     IGKIIHAKYHQDFGAIFDKVQVKIYTDEEKVREILEAARSSYRQRDQRIEGMTDENVS
                     TFYSCTLCQSFAPSHVCIVSPERTGLCGAYNWLDCRAAYEINPQGPNQPIQKGECLNE
                     QYGQFKGCNEYVRNASRQKIESVSLYSLLVDPMTTCGCCECIAAILPVANGIMTVDRD
                     FTDMTPCGMKFTTLAGAVGGGVQNPGFLGHSKYNITQKKFLKAEGGLLRIVWMPRRLK
                     EEIWERLSKRGEELGIQNLPDMIADETSARTEEEVIAYIAEKSHPCISMDPLI"
     gene            complement(6905..>7738)
                     /locus_tag="PK125_11505"
     CDS             complement(6905..>7738)
                     /locus_tag="PK125_11505"
                     /inference="COORDINATES: protein motif:HMM:NF015050.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB38770.1"
                     /translation="PEHGFDTAKKILKLAIENYPNRRHEVYIPEYSQDIVAGFSHETI
                     NYLLGGLFRASYRPLNDNIINGRIRGVAGVVGCNNVRTIHDSGHITMIKELVKNDVLV
                     LTTGCAAMACGKAGLLVPEAASKYAGSGLAEVCETVGIPPVLHMGSCVDNSRILIAAT
                     AMVKEGGLGDDIGDLPVAGAAPEWMSEKALAIGQYFVASGVYTVFGTTWPTMGSDRVT
                     EHLFEEYEDMFKGMWAFEPDPVEGAKLMISHIEKKRKALGIDKSRERVLFDMAMRRAI
                     E"
BASE COUNT         2078 a         1824 c         1555 g         2281 t
ORIGIN      
        1 tgactggttt atatccttaa accataatcc tttcccctgg tacacatatg ggggtgttga
       61 cctgtctaac gatgataggg ttggattatt tgtcgaagat cattactaca cgagaacaga
      121 tgctgctgtt gtgttcaaac gcattgaccg ctggaccggt aacgaacagt acatttacca
      181 cggcaatgat gggacaagca tgccatggaa tgacactgca caactgaatt acctaaaccc
      241 agaaatgcgt gaagctatga ttcaaaccat tcttcatgtg gcaaagaaat ttcctgtaat
      301 ccgtttcgac gcagccatga cgctgacaaa acgtcatttt cagagactgt ggtttccaca
      361 gccgggaaca gggggtgcta tcccgacacg ggctgagttt ggcatgagca aggaggagtt
      421 tgatagagcc atgcccaatg aattctggag agaagtcgtg gatcgtttag ctatagaagc
      481 accagatact cttttgcttg cagaagcgtt ttggcttctc gaaggatact ttgtccgcac
      541 cctcggaatg caccgtgttt acaatagtgc ctttatgaac atgcttcgag acgaagacaa
      601 cgccaagtat cgtatggttt tgaaaaacac actggaattc gatcccgaag tattgcgccg
      661 ttttgttaac tttatgaaca atcctgatga acgcactgca gtggatcaat ttggcaaaga
      721 caacaaatat tttggcattt gcaccctcat ggttaccctg cctggtcttc ctatgttcgg
      781 ccatggacag atcgaagggt acacagaaaa atatgggatg gagtatcgcc gcgcatactg
      841 gaatgagcat ccagaccaag cccttataga acgtcacgaa agagagattt ttcctctttt
      901 gcacaaaagg catttgttcg caggggtcga gagttttctc ttatacgact tctttaacct
      961 cgatggaaaa gttaatgagg atgtgtttgc ctattccaac agtttcgggc cagaaaagtc
     1021 acttgtggct tatcacaata aaaacagcca tgcttctggg tggataagaa catctgttat
     1081 gtattcagca aggaatcttg aagatggtac gcgaatcctc atgcagaaga ctctgagtgt
     1141 tggtctggct attccaccct ctggcaatcg cttcacagtt ttccgtgatc atcagtcagg
     1201 acttgagtac attcgacata ataacgatct ctgtgaaaaa ggtctctata tagagctagg
     1261 tccgtatgag tatcacgttt tcattgattt cagagaatat tcagacgatg aacaccatca
     1321 ttatgctcgt ttaacagact gcctgaacgg aaagggagtg ccaaacattg atgaagcact
     1381 ccgagaaatt tttctccaac caatccatca cagcttctcc caactctcaa acccaggttt
     1441 tttgaggtac ttgtggagca tccggggtac tctgatgaac aaacagattg actcaatacc
     1501 gccagatgtt ttgtctgaca tacgggtcag attcggcaac ttgttttcag aagtaatgca
     1561 tcatacaaaa ggcaatggca aaatagagga gctggcaaca aatacaagct ctatgattaa
     1621 ttgtcttatt acaggtattg acttacaggc aaacgttcct caggatatta aagataactt
     1681 cctgcaaaac atcttcgata tcacagagaa tctcaaagac gatgatctgg ttttctacac
     1741 actttgtcac tggacgtttg ttcatgctct tggagccatt gtttttgaaa ccggaagaga
     1801 tgcgcgagaa cttagtagaa gctggattga tgaatggttt cttggcaggt ttatcagtca
     1861 gactttaata gacttaggtt ttaacagaga gacagtatcc cgtgaagttg ctgttgtcaa
     1921 acttctcacg agttatcagg attggtataa ggatttcaca aacacctatg atcttttttc
     1981 aaaccttctg cgagacaacg aagtacgtga ctttttacag ataaaccgcc acctcgatat
     2041 tctatggttt aataaagaag cctttgaagg gcttatcttc tggatgatcc ttgttgcggt
     2101 agtgaagatc agttgcgaag caattaaaga ggaaaataaa gaggccttgg caagaaaagc
     2161 agtctgtctt atggaacccc tttatacagc tttggaaaat tctcagtatc aggtagaaaa
     2221 attgttaggg cttctaagaa cttacagctc tagccagaaa tccgaataga ggttttgtcc
     2281 gaggagaaca cgggttgtct ttacatattt tatctcctcc tcagaaagca ctgaattgtc
     2341 tatatcgtct ccgtccatca cactgtaaac tagcttttca agttcaggcc ttcgcccttc
     2401 tgcaatattc gtcaactctg aatcaaatgc atcgactata gctgaatata tcccgtaacg
     2461 tttgagcata atcaggtaag ttctgtttag cattcctctt aaattcctcg gaacaccatt
     2521 tgatatattg gagcctccat atatggattt acattctgga aaggcctctc taatcatcag
     2581 agcaaattct gtgcatcctt gaatttgtag ctgctgggtt ttaatagggg gcgcaatagg
     2641 gtctagccag atgtcttctt ctggtatgtt gtattggaat gcttcagcaa taatttgcgc
     2701 agcaaaaaag cctctttcat ttgcatctct gggaaaccct tcgtcaccga gggtaagacc
     2761 gacaaatgca gcattatatt ttttcgcaag aggcaataaa gcggccatgc gttctggctg
     2821 tgctgaaatc gagtttatca gtgctttgcc ttcatgaact tgtagacccg tttcgatagc
     2881 ttttatgttt gtcgtgtcaa gggaaagagg caaatccgtt acctcacgga ctgccctgac
     2941 aacccaactc atcatctccg aacctatctt aggtgcaggg ccaatattga tatcaagcat
     3001 gttcatacca gctttggctt gggcttctgc aatttctctg attatcttcc tgtccttatt
     3061 ctttagagcc tctcccacct ttttcgagct cacattaagg ttttctccta taagtatcac
     3121 acgttaactc cctcgaacct agactattag ctgtcaatga ttattgaata acacaatcaa
     3181 attaaaaggt tttttaactg ccctcctgtg ataacagcta aaacaaaaaa tgttttaaat
     3241 ctgttagctg ttggcaggaa attgtaagct gattcatttc cacattttca agtaagctgg
     3301 aatgtggact gcttcacgtg gtccaacttg aatctcccag ccaggtagtt cctcctccac
     3361 ttcaccgagt atcgacgctg catacccagg aattatgagt tttttatgtt ctatcttttc
     3421 aattatgccg cattttttga taaacgaacc tacaccatcg ccaagaaatt tgccagctgc
     3481 ccatgcagta agaactgaaa gcccttcagt gtccatgaca cagagccaac taggaatacg
     3541 tgaattttca atttctccac taacaataaa ataggtgagt gagaagttgc acgttattag
     3601 aaccggtgaa gcactgtcag gattatttat tggatagata ccttgctctg tcgtcattgg
     3661 cctctgggga tccgtataaa tattgaggcg ctgcaaaaga aggggaaaga tactctctcc
     3721 cttaaaatct gaaagcacta tgataccggc atacttcgcc acgaaacatg atgcaataag
     3781 agtttctttc atcaacgagt ctgtcatctc gcacggaaag gtaatagtag gaaaacctag
     3841 ttgtttattc ttgcccacca atgcagctct ccggatgatg atctggtctt gaaagctttc
     3901 tcttacaccc cgtgaggaag tgtccagtac aaggtctttc acacccatcg cagtgagttg
     3961 ttctgtcaaa tgcataactt cctcaatgcc attaccttta acagctaagg ggcagttgaa
     4021 ggctttggca agaaaaccga gttcctgaac attttcagtg gttgctgcat agataagtgg
     4081 ttttcttgcc ttgcagattt caaccgcttt tttcatcaat gaaattatag ggcatatcaa
     4141 aacgatcccg tatggctttt caattgcttt cttgacaagg ttaataaatc tttcatcatt
     4201 gcctgttgct ttcaatgcaa caagctcggg gcgtagtgtt gttcctatgc gatcatactg
     4261 gcaaaagttt accttctcga aacgactctc tatttcagcg tcatccattg tgtcggaaat
     4321 gagaattgca aatcctggct gatttaaaaa cgtcttttcg tgtctgaata atactgtttc
     4381 gcctccaatt ttaatttcat aatcactact tcctatacta acttgtcgaa taggtggggc
     4441 gcttgcctct gaaagttgca atcgcccttc atcggtgatg taaggacatg catcaatctc
     4501 ggccttactc gaggccaaag ccattgcaaa agccaggcaa gtcgggtatt tgcattcacc
     4561 acaatttgtt ttggggagaa gcttaaatat ttgaattcct gtgagcgcca ttttttctcc
     4621 ttttccaaaa tccgaattgt tccatacatg atcgggtcct gctcaatgca gattttttaa
     4681 tcttatatca agggatccat acttatacag ggatggctct tctcagcaat gtaggcgatc
     4741 acttcttctt cagtacgtgc agatgtttca tcggcaatca tatctggcaa gttctgaata
     4801 ccaagttcct cacctctctt gctgagcctt tcccaaatct cttctttgag cctcctgggc
     4861 atccagacta ttctcagcaa acccccttca gctttcaaaa attttttttg agtgatattg
     4921 tatttactgt gtccgagaaa gcctggattc tgtacgccac cacctacggc acctgccaag
     4981 gttgtaaact tcatgccaca gggtgtcatg tcagtaaaat ccctgtcgac tgtcatgatg
     5041 ccgttggcaa caggaagaat ggcggcaatg cactcacagc agccacaagt tgtcatagga
     5101 tcaacaagaa gactgtagag gctcacgctt tcaattttct gccgcgatgc atttcggaca
     5161 tactcattac accctttaaa ctgcccatac tgctcgttca aacattctcc tttttgtatg
     5221 ggctggtttg gcccctgtgg attgatttca tacgctgccc gacaatcaag ccagttatat
     5281 gcgccgcaca gaccagttcg ttcggggctg acaatgcaca catgactcgg tgcaaaggat
     5341 tggcagagcg tgcatgaata gaaagtgctc acgttttcat cagtcatacc ttcaatcctt
     5401 tgatctctct gcctgtagga cgatctggct gcctcaagga tctcccgtac cttttcttca
     5461 tctgtgtaaa tcttaacctg taccttgtca aaaatcgctc caaagtcctg atgatatttg
     5521 gcgtgtataa tttttcctat atgctgtagt ttaaaacctt tttccacagc ctgcttggaa
     5581 attcttatcc atgcaatatc tctttgtcct atatgcatga taccttgggc ttgattaatc
     5641 aaatggtggt tctgcctttc aagaataggc tcgaaatctt cttgaaaatt tctgcctgcc
     5701 acctctgcca cgagggcaaa atgtgcctgt gaaccgacgt ggaactgatc gatattggga
     5761 ccaataacct caaccctacc atcttcgact tcttccatct tctttgttat agtccactca
     5821 actgctatcg ttcttccacc acccatctct aaataaatat cttcacccct aacacgttcc
     5881 ccctcgaagg ctggtccata tgatacggga ataggcaccc ttgccacatg aactttaagc
     5941 cctctcacct ctactgcctt ctgaacaatc tgatggtggg gaatattaga aaccacgtgc
     6001 tcgtatgtgc aaataccggt cggcaatact tgtgggattg gcgtatcagc tatagttggg
     6061 aatccccagt tgatagcacc tgctgcattt gcataccatt cgtccgttac attgccgagg
     6121 gcaaggacaa atgcgtatgt tcgatccttg ttgtatatga gatttcttct gaaatccccc
     6181 ggccttatgc ctccgaaggc catggccaca cggcatgcaa aacctatggc aaatacagcc
     6241 tggtatatat ctggtccgaa tgggaccagc ctcgttggcc agcctatttg gaccccagtt
     6301 tctgcaagtt gctctgaaat tctttttcca tcattttctc cacacatgaa aacataaaga
     6361 ttcttttcct gcagctcgag ggcaattttt gcggcagttt tagaatccgg tgctgcacca
     6421 actatagccg caaaacctgg agctgtccca tcaacaaact cgacacccct tttccgaaaa
     6481 ataacatcat ttgcagcgcc gagccaatta gagctctccg tgagatcttc gctgtttgca
     6541 gcataaaaat ctggcgtttc aagatacttg atggcttcca gtatctcttc tgcgaaaaac
     6601 gttgccattc ctgcatcaag agccggagca agataaggaa gggaagatat ctcccgaaca
     6661 gggggaggaa taagcctctt gcagatatcg agtacctttc tcatatcccc aatcctttta
     6721 attccaattc cggttatcgc gtaaataatg ggaaggtaat aggctgtgtt tgggaagcct
     6781 acctcctggt ccggtccaaa cctctccagg gcatctttgt atctttcctc tgttttatta
     6841 taaattttat gtgcccctcg aatagctgcc gatgctatga ttttggacaa taatctcctc
     6901 ctttctattc tattgcccgt ctcatggcca tatcaaaaag caccctttct ctggatttat
     6961 caatgccaag tgccttcctc tttttttcaa tgtgagaaat cataagtttc gctccctcaa
     7021 cagggtccgg ctcaaacgcc cacattcctt taaacatgtc ctcgtattct tcaaaaaggt
     7081 gttcggtaac cctgtcgctt cccatagtag gccaagtagt gccaaacact gtgtaaacac
     7141 cggaagcaac aaaatactgc cctattgcaa gagccttttc actcatccat tccggcgcag
     7201 cacctgcaac aggcagatcg cctatatcat cgccaagtcc cccctctttt accattgctg
     7261 ttgcagcaat aagaatcctc gagttatcaa cacatgaccc catgtgaaga accgggggaa
     7321 tgcccactgt ttcgcatact tctgctaacc ccgatccagc atattttgaa gcggcctctg
     7381 gaacaaggag gcctgcctta ccgcatgcca tagcagcgca acctgttgtc aaaacgagga
     7441 catcattctt aacaagctct ttaatcatgg ttatatgacc agaatcgtga atagtcctca
     7501 cattattgca ccccactaca cctgccacac cgcgtatcct tccgttaatt atattatcat
     7561 taaggggacg ataactggcc ctaaaaagac ctccaagaag atagtttatc gtttcatgac
     7621 tgaatccagc gacaatatct tgtgaatatt ccggaatata gacctcgtgt ctcctgttgg
     7681 gataattctc gattgctaat ttcagaatct tttttgcagt atcaaagcca tgctcagg
//
LOCUS       DAOOMC010000130         7725 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdus sp. isolate AMR_MDS_2685
            NODE_11138_length_7725_cov_6.069231, whole genome shotgun sequence.
ACCESSION   DAOOMC010000130 DAOOMC010000000
VERSION     DAOOMC010000130.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864760
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdus sp. (wastewater metagenome)
  ORGANISM  Syntrophorhabdus sp.
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae; Syntrophorhabdus.
REFERENCE   1  (bases 1 to 7725)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7725)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,729
            Genes (coding)                    :: 2,708
            CDSs (with protein)               :: 2,708
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 7 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7725
                     /organism="Syntrophorhabdus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11138_length_7725_cov_6.069231"
                     /isolate="AMR_MDS_2685"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2699756"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(709..1533)
                     /locus_tag="PK125_11510"
     CDS             complement(709..1533)
                     /locus_tag="PK125_11510"
                     /inference="COORDINATES: protein motif:HMM:NF037411.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB38771.1"
                     /translation="MKIILSRKGFDSENGKFPSMILPSGMLLSLPIPHQGDRTLFSEL
                     TCEGVSVLNLMHQLGMTEFNTETKCHLDPDINADMIARPKGWKGAFGQMGGAQGHLEN
                     NGVGIGDLFLFFGWFRKTEEDNRSMLRYVGPHIHLIYGYLQIGEILKVMNSTSLPDWL
                     IDHPHTNRKKYLHLQGGSNNTIYVATDELTFLKGIPGYGCFKFHPKLVLTKPGESRTR
                     WDLPKEFQGLKITYHSDSSWKDGYFQSAAKGQEFVIEDSPIAEKWGKAIIEAGLEK"
     gene            complement(1620..2546)
                     /locus_tag="PK125_11515"
     CDS             complement(1620..2546)
                     /locus_tag="PK125_11515"
                     /inference="COORDINATES: protein motif:HMM:NF024676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HPB38772.1"
                     /translation="MSDKFNALWIILNKLDKKEKVTARSLMEDLEVSERSVYRYITTL
                     QVAGFPVDYDRAVNSYVFSDGYTLKKPDISVEETLALALSRKLLKSFGPDMERSLAKI
                     EEKLSVKKPEDLRHIVLDADAIAPSETFLTLAHRAVINHQRINLKYKALHARTPRESK
                     IDPYYLFFRDGIWYFRGYYHRDRAMRTFAFDRVVSLSLLNEYFVPQPVSPENELSGAF
                     GAFLDGEPVEVTLQFDKHIKPFIVRKKWHESQKVKKLDNGKIEVKFKVNGLMGIKNWI
                     YGWIPHVEVIAPRELRTALKRDLQKAIKLHGK"
     gene            complement(3004..3243)
                     /locus_tag="PK125_11520"
     CDS             complement(3004..3243)
                     /locus_tag="PK125_11520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_177722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dodecin family protein"
                     /protein_id="HPB38773.1"
                     /translation="MSVYVVRQIIGTSTKSWEDAARQVVKTATKTIKDIRIGEVVKQD
                     VTVENGKVVTYRVRLNVSFKYHQQWWDRSDWECIS"
     gene            complement(3512..5362)
                     /locus_tag="PK125_11525"
     CDS             complement(3512..5362)
                     /locus_tag="PK125_11525"
                     /inference="COORDINATES: protein motif:HMM:NF006153.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-utilizing enzyme"
                     /protein_id="HPB38774.1"
                     /translation="MNVTFIDPHEVPTIPGTEGWERLYPYHYLFSRQDPKRAAFESNQ
                     IWFYDSLHYPDPHHPFDLIWDEAWFLALSQYNTRHYLMPSSLGIDHRIVNGYVYVSPV
                     CLSDPEKIQSRVPYFVERAGYYYENWDRLYENWRKKIAQVIEELEGMNFTDLPEMEDV
                     SVIKNGIGKGSGFDLLKKYDDIINLGLLCWQYHFEFLTLAYAAQVSFVKTVDQIFPKI
                     PIPALIKMSASIESIVCRPEKELVKLANLAIDLAIDEVFVRFKNAADVISEMGKSQAG
                     QTWLKELEQSRYPWFYISSNTGWHHTHLSWNDNLDIPFEIIGANIKSIKSGKKLERST
                     NLIEQERDSIANEYRKLIRNEKDRVAFDQSLALARMVLPYSEDHTFYVEHWFHSIFWR
                     KIRELGKVLERAGFLENGEQDIWFLKRDEVKQALWDYTTAWATGVSPRGPTYWPAEVA
                     WRKEIHEKFRTWTPPPALGKAPETITEPFTIVLWGVTKEGLKNWSEADKKRADDVSSN
                     VIQGMPGASGIAEGRARVLKSIDDLADINEGEILVVATTTASWVQAFFKIAGVVTDIG
                     GPMSHAAVICREYGLPTVVGTGKGTQLIKTNDLIRINGNTGQVTILERNN"
     gene            complement(5589..7148)
                     /locus_tag="PK125_11530"
     CDS             complement(5589..7148)
                     /locus_tag="PK125_11530"
                     /inference="COORDINATES: protein motif:HMM:NF002674.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tm-1-like ATP-binding domain-containing protein"
                     /protein_id="HPB38775.1"
                     /translation="MKTILVVATLDTKGMEVAFSKENIEALGAKALLMDAGILGSPTI
                     TPDITREEVAVAAGSTLKKVRKMTAEAEALGVMTKGASNIAVKLHQEGKIHGVLGIGG
                     GMGTAMGTGVMRSLPIGVPKFMVSPQAGNPDVVSAAVGTKDICMFHSVTDVVGLNPLL
                     RTIFSKACGGVVGMANAEVRLETNYRKTVAVCAKGTTEAANQAIRDKLTEAGYQPMTF
                     HCFGYGPASLEQIIKDGYIDGGVIELASDWLDRIGGGASFPPADRYENAGELGIPIVF
                     VPGSCDFIAAAPGKFPGRTVQPHNRAVSLYRSSKEELAQLAKEVGEKLSKSKGPVTVL
                     IPLKGFCVHDREGGPLYDPEANAAFIEGISAFEGKFKIKKVDAHINDEKFVDAIIEAF
                     KENIEEAIPVTSHPITDKVILTLRGYAASSGLAVGPCKKITHIDDVRGFDDESILVVK
                     TASPALVRLIPGLKGLVCEQGGPLSTASIFAREHGVPAAVGVNGVFDALKDGDLIRLD
                     GAKGTIDVIAR"
BASE COUNT         1896 a         1844 c         1665 g         2320 t
ORIGIN      
        1 aacccctggc tgggagacaa tgctgccaac catacctgat gtgcctggct gtgtatcaca
       61 ccagttgtaa ccctggtgat agatgaagag tgcatgttga gggaaggcag aaatcttcgg
      121 atactataca cacatttagg atacaaagta agatacgtca caaatgagga tttcagttca
      181 ctactgccat tggaataatt ggataaaacg attaacgagg ccctggaacg aatgtttaac
      241 aggatgcatg tgataattca ttgctcagca ggtatttgcc gaatggtact ctttgcagtc
      301 tgtctatgag attgggagga atgttttgtg agtgcatatc ttggaaaggg tttgcaacaa
      361 taattttgtc attatttggt cgataaatta catgtataga attaacgtcg tcacgtactg
      421 tgagatgtcg ataaggtttg aaggtatacc cttttttggt tgaatggaca tggatcagat
      481 gatgactgaa caatctgaaa aaatcttttc agatgctgac aataccttct taagccatcc
      541 ttttctcatt ttgacgcctt cgaaattaaa ccaatttgtt agaagaactt acccttattt
      601 ttatagttat gtgggctatc ataaatatac aggcccggta ttattctgtt taatccatac
      661 catatcttca agactcttgg cacagtacct ccgctatggt tttgcgggtt atttctccaa
      721 accggcctca atgatagctt tcccccattt ctctgcaata ggtgaatctt caatgacaaa
      781 ctcctgcccc ttggcggctg attggaaata gccgtctttc caactgctat cagagtgata
      841 tgtaatcttc aagccctgga actcctttgg caggtcccac cgagttcttg attcacctgg
      901 ttttgttaaa actagtttag gatgaaactt gaagcagcca taccctggga tacctttgag
      961 gaatgtcagt tcatcagtgg caacatatat cgtattgttg ctgccgcctt gcagatggag
     1021 atacttcttg cgattagtat gtggatggtc aatcagccag tctggcaagc ttgtactgtt
     1081 cattaccttt aatatttcac caatttgaag ataaccatat atcagatgaa tatgagggcc
     1141 aacgtatcta agcatgcttc gattgtcctc ttcagtcttt ctaaaccatc caaagaataa
     1201 gaacaaatcg ccaatgccaa cgccattgtt ctccagatgg ccttgggctc cacccatttg
     1261 accaaatgcc cctttccatc ctttcgggcg tgcgatcata tccgcattga tgtcaggatc
     1321 taagtggcac ttggtttcag tattaaattc agtcatgcct aactgatgca ttaaattcaa
     1381 cacactgacg ccttcgcagg tcagctcaga gaaaagcgtc ctatcacctt gatgaggaat
     1441 ggggagtgaa agcaacattc ctgaaggcaa aatcatactt ggaaacttcc cattctcgga
     1501 atcaaaccct ttcctactaa gaattatttt cataaccttc tattctccat taatcatatt
     1561 cactgacggt ccaggttttt caggacctcg tagaccagcc gccaaaatct cagagtagct
     1621 tacttgccat gcaatttaat cgccttttgc aaatcccgct tcagagctgt ccgtaattct
     1681 ctgggggcta tgacttcaac atggggaatc cagccgtata tccagttttt tatccccatg
     1741 aggccgttga ccttgaactt cacctcaatc ttcccgttat ccagcttttt aaccttctgg
     1801 ctttcgtgcc acttcttccg cacaatgaag ggcttgatgt gcttatcaaa ctggagtgta
     1861 acttctacag gctcaccgtc gagaaatgcc ccgaaggcgc ctgagagttc attctcagga
     1921 gagactggtt gaggaacaaa atactcattt aggagactta acgagactac ccggtcaaag
     1981 gcaaatgttc tcattgccct gtctctatgg taatatccac ggaagtacca gataccgtcc
     2041 cggaagaaaa gatagtaggg gtcgatcttg ctctctctgg gtgtcctggc atgaagggcc
     2101 ttgtatttga gattgatccg ctggtgattg ataactgccc ggtgggccag ggtcaagaac
     2161 gtttcagaag gtgctatggc atcagcatcg aggacaatgt gccttaggtc ttctggcttt
     2221 ttaactgaca gtttttcttc tatctttgcc aggctacgct ccatgtcagg gccgaaactt
     2281 ttgagcagtt ttctggataa agccagggca agagtctcct ccacagagat atcaggtttc
     2341 ttcaacgtgt agccatcgct gaaaacatag ctgttcactg ccctgtcata gtctactggg
     2401 aaaccggcaa cctgtaacgt tgtgatatac cggtaaacgc tcctctcgct gacttcaagg
     2461 tcctccatga gagaccttgc cgttaccttt tccttcttgt caagcttgtt gagaataatc
     2521 catagtgcat tgaacttgtc tgacataatc ccccctttcg tgtaccatta atatatcata
     2581 tcaggctgcc atgtgtatgg caggatgttg aagtcgcatc aatttacgat ctcaagacat
     2641 ttgtgaatta ctttaaatcg ttccattaaa caattggtaa gactattcat gatgtccttg
     2701 cagcaagtat ttgtcttggt ttatgcaata tgaccggacg tcctcaaaaa agaagccgca
     2761 gtaaagagat tgtcaaaggc gggtaaaatg tgtctgcctg aaagctgtat tccagtggaa
     2821 cgacctgaca aaagaccaga atccattggg caataacctt tcgggttgtt cagcaacacc
     2881 ttgtcttttt tttcaatttg tgtgtctcga tatgggaata aacatattct aaaagatact
     2941 caaatattct gatgaaatca ttttaaaggg ccaatgcaca actgttgttg cattgttacg
     3001 acctcagcta atacattccc agtccgacct gtcccaccac tgctgatgat atttgaaaga
     3061 cacattgagt cttacacggt aggtcacaac cttcccgttc tccactgtta catcctgctt
     3121 taccacctca ccgatgcgga tatccttgat ggtctttgta gcagttttga cgacctgcct
     3181 ggctgcatcc tcccatgatt tcgtgctggt gccaatgatc tgtctcacca catatacgct
     3241 cacaatagcc cctcctattt tattgtggtt cttacgatgt tataggtgtt aatagcacaa
     3301 agaaatgata tgtcaagtga atggtaactt ttggcctttg catgacgatg agaccccatt
     3361 ttcgccatat agccatggcc tgacgtatac acacttttag catgatgttc ggggctatgt
     3421 ttttaggaag agattgttct aagtggtgcc taaaatattg agcgtcagtg aattaggatg
     3481 tgtgtgtcta attttattga aaacaccata gtcaattatt ccgttccagg atagtgacct
     3541 gcccggtgtt tccattaatc ctaatcaggt cgtttgtttt gataagctgt gttcctttac
     3601 cggttcccac gacggtgggc agtccgtact ccctgcagat gacagcggcg tgagacatag
     3661 gccctccgat gtcggttacc acacctgcga tcttgaaaaa agcctgcacc cagcttgctg
     3721 tcgtggtggc cactacgagg atttcaccct cgtttatatc agcgaggtca tcaatgcttt
     3781 tcagaactcg ggctctgccc tctgcaatgc cagaggcacc gggcatacct tgaatcacat
     3841 tggaagagac atcatcagct ctttttttgt ctgcttcgga ccagtttttc aacccctcct
     3901 tcgtcactcc ccatagaacg atggtgaatg gctcggttat tgtttccggt gctttcccga
     3961 gggcaggagg cggggtccag gttctgaatt tctcatggat ttcctttctc caggcgactt
     4021 ctgcaggcca ataagttggt ccgcggggag atacgcctgt tgcccaggcc gtggtatagt
     4081 cccatagtgc ctgtttaacc tcatcccgct tgaggaacca tatgtcctgt tctccatttt
     4141 caaggaagcc agccctctcg aggaccttgc caagttcccg tattttcctc caaaaaatgc
     4201 tgtgaaacca atgctcaacg tagaatgtat gatcctccga gtaagggaga accatgcggg
     4261 ccaaggcgag gctctggtca aaagcaacac ggtccttctc gtttcggatt agctttctgt
     4321 attcattggc aatgctgtcc cgttcctgtt cgatcagatt tgttgatctt tccagtttct
     4381 ttccagattt tatgcttttg atgttagcac ctattatttc gaaagggatg tcaaggttgt
     4441 cattccagct caggtgggta tgatgccaac ctgtattgct ggagatgtaa aaccagggat
     4501 atcttgattg ctccagttcc ttgagccacg tctgcccagc ctggcttttc cccatttcac
     4561 ttattacatc agcagcattc ttgaatctca caaagacctc gtcaatagcc agatcgatag
     4621 cgaggtttgc gagctttacc agctcctttt ctggcctgca tacaatcgat tcaatgcttg
     4681 ccgacatttt tatgagagct ggaatgggaa ttttaggaaa tatctggtca actgttttta
     4741 caaagctaac ttgagctgca tatgcgagag tgagaaattc aaaatggtac tgccagcaga
     4801 ggaggccaag attgataata tcatcgtatt ttttaagaag atcgaatcca ctccctttcc
     4861 cgatcccgtt ttttatgacg ctcacgtctt ccatttcggg caggtctgta aaattcattc
     4921 cttcaagctc ttcaataacc tgggcgattt ttttcctcca gttttcatag agcctgtccc
     4981 agttttcgta ataatacccg gctcgttcca cgaaatatgg tacccttgac tggatttttt
     5041 cagggtctga taagcagacc gggctcacat agacatagcc gttcacaatt ctgtggtcga
     5101 tgcccaagga agagggcatt aagtaatgac gcgtattgta ttgcgatagg gcaaggaacc
     5161 aggcttcatc ccatatcagg tcaaaggggt ggtgggggtc tggatagtgg aggctgtcat
     5221 aaaaccatat ctggttgctt tcaaaggccg ctctttttgg gtcttgtctt gagaataaat
     5281 aatggtaagg atacagcctc tcccagcctt ctgtacctgg gatcgtcgga acttcgtgcg
     5341 ggtcaataaa cgttacgttc acaacatttc tccttcccca tcgatattat accacaagca
     5401 ccctctttgt cactgtttcg gggccccatc tgtggaacaa atccgcatcg atgccgacca
     5461 tgccaggagt attgtccatc atctgtttta aggccgttga cggcattggg cataatggtt
     5521 gccttcacca acgctacttt cagctttccg cgaaattaca gggctggcaa tagttttttt
     5581 gaagcatcct atcgtgcaat cacgtctatg gtgcctttcg caccgtccag tcttatgagg
     5641 tcgccgtctt tgagtgcatc aaaaacgccg ttaacgccta ctgccgcagg cactccgtgc
     5701 tccctggcga agatgctggc tgtagagaga gggccaccct gttcgcatac cagtcctttc
     5761 aatcccggta tcaagcggac aagtgctggt gatgccgttt tgacaacgag gatcgattca
     5821 tcatcaaacc ccctcacatc gtcaatatgc gtaatcttct tgcaggggcc aactgccaat
     5881 ccgcttgatg cagcgtatcc cctaagggta aggatgactt tgtctgtaat tggatggctt
     5941 gtaacgggta ttgcctcttc aatattttct ttaaaggcct caatgatggc atcaacaaat
     6001 ttctcatcat tgatgtgggc atccaccttt ttaatcttaa acttgccttc aaatgctgaa
     6061 atcccttcga taaaggcggc attggcctca ggatcataga ggggaccacc ttcccgatca
     6121 tggacacaga accctttcaa tggtattaac accgttactg gccctttgct tttcgacaat
     6181 ttctcaccta cttcttttgc cagttgggca agttcttctt tactggacct gtagagtgat
     6241 actgcccggt tgtgaggctg aaccgttcgc cctggaaact tccccggtgc agccgcaata
     6301 aaatcacagg aaccgggtac aaaaactatg ggaatgccaa gctcacctgc attttcatag
     6361 cggtctgctg gaggaaaaga agcgcccccg ccgatacgat caagccagtc actggcaagc
     6421 tcaataacac cgccgtcgat atagccgtcc tttattatct gttccaggct tgcaggtccg
     6481 tagccgaagc agtggaatgt catgggctga tagccagcct cagtgagttt atcccttatg
     6541 gcttggttgg ctgcttctgt ggtacccttg gcacacacgg caaccgtctt tcggtaattt
     6601 gtttccagcc ttacctcggc gtttgccatt ccaacgacac ctccgcacgc tttagagaag
     6661 atcgttctca ataatggatt cagacccaca acatcggtga cagaatgaaa catgcagata
     6721 tcctttgttc ctactgctgc gcttaccacg tcgggattac cagcctgagg agaaaccata
     6781 aactttggta ccccaatggg gagactgcgc atgacacctg ttcccatggc agtacccatg
     6841 cctcctccaa taccgaggac tccgtggatc tttccctcct ggtggagttt tactgctatg
     6901 ttgcttgcgc cctttgtcat aacccccagg gcctcggcct ctgcagtcat cttccttact
     6961 ttttttaggg ttgaaccggc tgcaacagcc acctcctcac gggttatatc gggtgttata
     7021 gtaggggagc ccagtatgcc tgcatccata agaagtgctt ttgctcccag tgcttctatg
     7081 ttctctttag aaaacgctac ctccatacct ttggtgtcca gtgttgcaac aacgagtatt
     7141 gttttcatgc cttaccccct ttatattttc tttctcactg tgttgctgtt atgatgcatt
     7201 ttgttcccca aaccatcatg tacacctgat tgtggatggc gattactatc atgtatcaag
     7261 tataagctgt tgtgaataga tatgaaaata ggaaataaac tgtttaaaat aggatattta
     7321 agttcctatt ttaaagatcc cgtctccacg gtagaatttt gatgtaatct tttatctgga
     7381 tttgcttgac cacgggttta ggggtttttt aattcccatt cctttcttct ggctataatt
     7441 ccgtatattt cctgctatcc gattcacatc ggtgttcccc ctgtgtaaga gggtcatgta
     7501 attgagtgtg cattataaat aggagcccag ggataagata ggaacatttg ggtcatactt
     7561 ttgatgaagg gtagctgaaa aaaaaataga ttttcccttt gccgttaaag ggagaggagc
     7621 agggacctca gattaatctt gcccttattc gctcccagtt tttttcgtat aatgaatctt
     7681 tgtttatcaa tcgcgtcttt cgtagcaccg agtgtctctg ctatt
//
LOCUS       DAOOMC010000131         7577 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdus sp. isolate AMR_MDS_2685
            NODE_11438_length_7577_cov_5.983382, whole genome shotgun sequence.
ACCESSION   DAOOMC010000131 DAOOMC010000000
VERSION     DAOOMC010000131.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864760
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdus sp. (wastewater metagenome)
  ORGANISM  Syntrophorhabdus sp.
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae; Syntrophorhabdus.
REFERENCE   1  (bases 1 to 7577)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7577)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,729
            Genes (coding)                    :: 2,708
            CDSs (with protein)               :: 2,708
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 7 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7577
                     /organism="Syntrophorhabdus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11438_length_7577_cov_5.983382"
                     /isolate="AMR_MDS_2685"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2699756"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..848)
                     /locus_tag="PK125_11535"
     CDS             complement(<1..848)
                     /locus_tag="PK125_11535"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HPB38776.1"
                     /translation="MSILIRNGRVIDPKSGLDEVIDVFVNGNTIEKIDKHIRKTGSDV
                     RSIDASGHIVAPGLIDTHAHLREPGYEWKETIRTGTMAATKGGFTSVVCMANTNPVND
                     NKSVTDYIIQKARSEALCRVFPCGAITKGLKGEELSDMGELYESGIVAISDDGKSVKN
                     SAILLKAFEYSRLFGIPVISHCEDEDLSRGYVHEGRASLLSGLDAVPAIAEELIVSRD
                     IAIAKYANAPVHLTHISSGGSVNIIQHAKKSHKKITCDTCCHYFSLTDEATLGFDTST
                     KGNPPLR"
     gene            complement(845..1768)
                     /locus_tag="PK125_11540"
     CDS             complement(845..1768)
                     /locus_tag="PK125_11540"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HPB38777.1"
                     /translation="MNWTKKDLLGITELSKEEILFVLDTAESFKEISQRDIKKVPTLR
                     GKTVITLFYEPSTRTRTSFEIAAKRLSADTINISASTSSYVKGETLKDTAKNLEAMNP
                     DVTIIRHSMPGAPHMLARILDSSVVNGGDGAHEHPTQALLDAYTIREKKGTIDGLKIV
                     IVGDIIHSRVARSNIFTLRNFDSEIVCCGPPTMLPPHMEELGIKVEYNLKKAIHNADV
                     IMMLRIQKERGGVSYIPSVKEYSAFFGLTGEHIMNAKKDVIIMHPGPMNRGVEIMDDV
                     ADGPYSVILEQVENGVAVRMAILYLLLGREK"
     gene            complement(1765..2298)
                     /gene="pyrR"
                     /locus_tag="PK125_11545"
     CDS             complement(1765..2298)
                     /gene="pyrR"
                     /locus_tag="PK125_11545"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: protein motif:HMM:NF003549.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HPB38778.1"
                     /translation="MENRVLLDNKAVERTINRIAHEIIEKNKGVEKLRLIGIRTRGVH
                     LAQRLKQKIEEIEHISLPLGVLDITMYRDDVFKLKSPGLKKTEIPFDVNNITAILVDD
                     VMQTGRTTRAAIDAIMDLGRPKRIQLAVLVDRGDRELPIHPDYAGIVHSAHPEENVLV
                     RLSEVDNRDEVVVVTSS"
     gene            complement(2298..2477)
                     /locus_tag="PK125_11550"
     CDS             complement(2298..2477)
                     /locus_tag="PK125_11550"
                     /inference="COORDINATES: protein motif:HMM:NF024634.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HPB38779.1"
                     /translation="MRPYISKGLCNCCMECIDLCPYDVFREEEGEVNVANPEDCIECM
                     SCIELCPAKAIFMDD"
     gene            complement(2554..5757)
                     /gene="carB"
                     /locus_tag="PK125_11555"
     CDS             complement(2554..5757)
                     /gene="carB"
                     /locus_tag="PK125_11555"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: protein motif:HMM:NF009455.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HPB38780.1"
                     /translation="MKKIPGINRVLIIGSGPIIIGQACEFDYSGTQACKALKSIGYEV
                     ILVNSNPATIMTDPGMADATYVEPLTVEALEKIIEKEKPDALLPNLGGQTGLNLAAEL
                     SKKGILAKHKVQIIGVKADVIERGEDRIEFKNTMNKLGIPTPLSDPAYSIEEALAIAD
                     KLGYPVVVRPAYTLGGWGGGITYNKEELKTIASRGIAASLIGQILIEEAVIGWEELEL
                     EVVRDADNNMITVCFIENIDPMGVHTGDSFCSAPMLTISEEVQKRLQEYAYRIVEAIQ
                     VIGGTNVQFAHNPNTGRIVVIEINPRTSRSSALASKATGFPIALISAKLAGGYTLKDL
                     PYWRAGTLDKYTPWGDYVVIKFARWAFEKFKEAEDRLGTQMKAVGEVMSIGKNYKEAF
                     QKAIRSLETKRYGLGFARDFNRRPLQDLMAMLREPSSERQFIMYEALRKGATIEDLHN
                     KTMIKPWFIQQMKELVELEEKILAYKGKDLPDQLLIQAKEDGFSDKYLAQILDVSEED
                     VRNKRIALGKTGAWCAVPVSGADASYYYSTYNAPDEVKVSNRKKVMILGGGPNRIGQG
                     IEFDYTCVHAAFTLRDEGFETIMVNCNPETVSTDYDTSDRLYFEPLTVEDVLSIYHKE
                     KPIGVIVQFGGQTPLNIARELELAGVKILGTSPESIDLAEDRKRFKEVMTRLEIPQPE
                     SDTAVSLEEALTIAHDIGYPLLVRPSYVLGGRGMEIVYDDDMLTSYINEAVEIWPGRP
                     ILIDRFLENAIECEADAISDGEDAFVPSVMEHIELAGIHSGDSACAIPPRTISKKQID
                     IINEYTKKIAVELNVVGLMNIQYAISNDTVYILEANPRASRTVPLVSKVTGIQMARIA
                     TQVMIGKKLKDMGLKQKKYAHVGVKESVFPFNMFPEVDPTLGPEMKSTGEVLGMADTF
                     GLAFFKSQEGAGQKLPTSGTVLISVKQKDKKGILAVAKYLQSIGFKILATDDTHKFLS
                     ANGVNSSFIKKVHEGRPNIVDAIHNKEIDLIINTPIGKNSKYDDSYIRKAAIKYKIPY
                     MTTASAAESVAEAIKAYLQKKKGMTVKSLQAYHRDIL"
     gene            5889..6278
                     /locus_tag="PK125_11560"
     CDS             5889..6278
                     /locus_tag="PK125_11560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB38781.1"
                     /translation="MMNGVNENTTEVIGQGCKTQGSRLGLVRDLFIFWFVIFLVVLCW
                     TNNILVTAILIAAYGVRYYFWPNKEDHVLYIGGAILGPIAEVIATKVGIWQYTLPTFL
                     NIPLWLPFAWGLAAVLIIRIALAFARK"
     gene            6460..>7577
                     /gene="secD"
                     /locus_tag="PK125_11565"
     CDS             6460..>7577
                     /gene="secD"
                     /locus_tag="PK125_11565"
                     /inference="COORDINATES: protein motif:HMM:TIGR01129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HPB38782.1"
                     /translation="MFEPKGNLKKYLPSDKIHLGLDLKGGMHLLLELDMAKMMQNLSE
                     RKFTALKDAMIRDGVRFLALDMKGNTVSVAVKAEQKEKLYNLVGKEFPDIKAGTTKTE
                     GDTFFVDFVFSDKELTSLKDNAVRQALETIRNRIDQFGVSEPVIVKQGENQILVQLPG
                     IEDPQRALDLIGRTAQLEFKLVDEDNAGRASSGSVPDGDELLMMRTKNRETGIATTTP
                     ILLKKQALLTGELLTDAKVSIGGEFRNEPYVGIEFNSEGARIFDKITAENVGKRLAIV
                     LDNTVYSAPVVKERISGGRASITGGFTMEEAKDLAIVLRAGALPAPVKVVQNITIGPT
                     LGQDSIRKGIRATILGALLTIIFMIVYYRGCGVIANIAL"
BASE COUNT         1853 a         1741 c         1754 g         2229 t
ORIGIN      
        1 cgaaggggag gattgccctt ggtactggtg tcgaacccca gggtcgcctc gtctgtcagg
       61 gagaagtagt ggcaacaggt gtcacaggtg atttttttgt gggatttctt ggcatgctgt
      121 atgatgttta cgctaccccc tgatgaaatg tgggtgaggt ggacaggtgc attggcatat
      181 tttgcaattg caatgtcccg actcacgatg agttcctcag ctattgcagg gactgcatcc
      241 agccctgaca ataaggaggc cctgccctca tgaacgtaac ccctcgacag gtcttcatct
      301 tcgcagtgag aaataacagg tataccgaac aacctggaat attcgaaggc tttcaggagt
      361 atggcgctgt ttttaacgga ctttccatca tcggagatag ccacaatccc cgattcatag
      421 agttctccca tatctgagag ttcctctccc tttaatcctt tggtgatggc gccgcacggg
      481 aaaacacggc acaaagcttc agatcgtgct ttctggatta tgtagtcagt tacgctctta
      541 ttgtcattta cagggtttgt gttggccatg cagacaacgc tcgtgaaacc gccttttgta
      601 gctgccatgg ttcctgttct gatggtttcc ttccactcgt agcctggttc tctcagatgg
      661 gcgtgtgtat cgatgagacc gggtgccact atatggccag aagcatcaat ggaacgaaca
      721 tcactgcctg tcttccttat atgtttatca attttttcaa tggtattgcc attgacaaat
      781 acgtcaataa cctcatcgag gccgctcttc gggtctataa cccttccatt tctgatcagt
      841 atgctcactt ctccctcccg aggaggagat agaggattgc cattctcaca gccacgccat
      901 tctcaacctg ttcaaggatg accgaatatg gaccatctgc cacatcatcc attatctcga
      961 cacctctgtt catcgggcca ggatgcatga ttattacatc cttcttggca ttcatgatat
     1021 gctctccagt caatccgaag aaggcagagt actctttgac agacgggata tagctgacgc
     1081 cccctctttc tttctgtatc ctgagcatca tgattacgtc tgcattgtga atagcttttt
     1141 tcaggttata ttccactttt atcccaagtt cctccatatg aggaggaagc atggtgggag
     1201 gaccgcaaca gacaatttca gaatcgaagt ttctgagggt aaatatgttc gatcttgcca
     1261 cacggctgtg gataatatcc cctacaatga caatcttgag tccatcaatg gtgccttttt
     1321 tctctcgtat ggtatacgca tcgagaagag cctgggttgg atgctcatga gcgccatcgc
     1381 cgccgttgac aaccgatgaa tcaagaatcc gggcgagcat atggggtgct cctggcatgc
     1441 tgtgccgaat gatcgtcaca tcagggttca tggcctcaag gtttttggct gtgtccttga
     1501 gagtctctcc ttttacatag ctgcttgtac ttgcagagat attgatggtg tctgcagaaa
     1561 gcctttttgc agcgatctca aatgatgttc ttgtccttgt gctgggttcg taaaaaaggg
     1621 taataactgt cttgccacga agagtaggta ctttcttaat atccctttgc gatatctctt
     1681 tgaaggattc tgctgtgtca aggacaaaga gtatctcttc ttttgatagt tctgtgatac
     1741 cgagtaggtc tttttttgtc caattcatga ggatgtcaca actaccactt cgtctctgtt
     1801 gtctacctcg cttaacctta ccagcacatt ttcttcagga tgggctgagt gaacaatacc
     1861 agcatagtca gggtgtatag ggagttctct gtcacctctg tcaacgagca cagcgagctg
     1921 gattctcttt ggtctgccca ggtccataat agcgtcgatt gcggctctcg tggtcctgcc
     1981 tgtctgcatg acatcatcaa cgagaatagc tgtgatattg ttgacatcaa agggaatctc
     2041 tgtttttttc aatcccgggg atttgagctt gaagacatcg tcccgataca ttgtaatgtc
     2101 aagcaccccc aggggcagtg agatatgttc aatctcttct attttctgtt tcagtctttg
     2161 ggcgaggtgg acccctcttg tccttatgcc gatgagcctc agtttttcaa cgcccttatt
     2221 tttttcaatg atctcatggg ctatgcggtt gatcgttctc tccaccgctt tattgtcgag
     2281 gagcacccta ttttccatca gtcatccata aagatagctt ttgcaggaca cagttcaatg
     2341 caggacatgc actctataca gtcttcgggg tttgccacgt tcacttcacc ctcttcttct
     2401 ctgaaaacgt cgtaagggca gaggtctata cattccatgc agcaattaca caaacctttg
     2461 gatatatatg gcctcaagct tcacactact ataaagggtt tatttaaaaa aatcaaaaaa
     2521 atcgattcaa aaattttcag tgttttggtg atttcataaa atatcccggt ggtatgcctg
     2581 cagtgatttt actgtcatgc ctttcttttt ttgcaaatag gcctttatgg cctcagcgac
     2641 tgattctgcc gcggaggcag ttgtcatgta aggaattttg tattttatcg cagctttcct
     2701 gatgtaagaa tcatcatatt tactgttttt gccgatgggt gtattgataa tcaaatctat
     2761 ctctttattg tgaatggcat ccacaatatt gggccttccc tcatgaactt tcttgatgaa
     2821 gcttgaatta accccattgg cgctgagaaa cttgtgggta tcgtcagtag caagaatctt
     2881 gaaaccgatg ctctggagat attttgcaac agcgagaata ccttttttgt ctttttgttt
     2941 aaccgaaata agtacagttc cgcttgtagg cagcttctgg ccagcccctt cctgagattt
     3001 gaaaaaggcc aatccgaatg tgtcagccat gccaaggacc tcgcctgtag atttcatttc
     3061 cggtccgagg gtggggtcaa cctccgggaa catgttgaag gggaatacag attccttgac
     3121 cccaacgtgt gcgtattttt tctgtttcag gcccatatcc ttcagcttct ttccgatcat
     3181 tacctgggtg gcaatacgtg ccatctgtat gccggtgacc tttgaaacga gaggaacagt
     3241 ccgtgaagcc ctggggttgg cttcgaggat gtacaccgtg tcattggata tagcgtactg
     3301 gatattcatg aggcccacaa cattcaattc aacggcgatt ttctttgtat attcattgat
     3361 gatatcgatt tgctttttgg aaatagtcct gggaggaatg gcgcaggcgc tgtctcctga
     3421 atggatgcct gcaagctcaa tatgttccat gacagaggga acaaatgcat cttctccgtc
     3481 tgagatggca tctgcctcac actcaatggc gttttcgagg aacctgtcga taaggattgg
     3541 acgtcccggc catatttcga cagcctcgtt gatatacgaa gtgagcatgt catcatcgta
     3601 aactatctcc atgcctcttc caccaaggac gtaagaaggt cttacaagca agggatagcc
     3661 tatatcatga gctatggtca gggcctcttc gagggagaca gctgtgtcac tttctggttg
     3721 ggggatttca agacgtgtca ttacttcttt gaaccgcttc ctgtcctcgg caaggtcaat
     3781 gctttcggga gaggtaccaa gaatctttac tccggcaagt tcgagttccc tggcaatatt
     3841 aaggggagtt tgtccaccaa attggacaat gacgccaatc ggtttttcct tgtggtaaat
     3901 gctgagaaca tcttcaactg taagaggctc aaaatagagt ctgtccgatg tgtcatagtc
     3961 tgtagagact gtttcgggat tacagttgac cataatggtc tcaaagcctt catcgcgaag
     4021 agtaaaagca gcatgcacac atgtgtaatc aaactcgata ccctgaccaa tacggttcgg
     4081 tccgcccccc aggatcataa cctttttcct gttgctcacc ttaacttcat caggggcatt
     4141 gtatgtagag tagtaatagg atgcatcagc accgcttacc ggaacagcgc accaggcccc
     4201 tgttttgcca agggcaattc gtttattcct cacatcctcc tctgaaacat caagaatctg
     4261 agcaaggtat ttatcagaaa aaccatcttc ctttgcctga atgaggagct gatcaggcaa
     4321 gtcttttccc ttataggcca gaatcttttc ctcaagctcc acaagctcct tcatctgctg
     4381 aataaaccat ggtttgatca tggttttatt atgaagatct tctatggttg cgcccttccg
     4441 caaagcttca tacatgatga actgtcgttc tgatgatggc tccctgagca tagccatgag
     4501 atcctgaagg ggacgcctgt taaaatctct ggcaaagccg aggccgtatc tttttgtttc
     4561 cagggaacgg atcgcttttt gaaatgcctc cttatagttt ttgccgatac tcataacctc
     4621 gcccacagcc ttcatctgtg tgccgagtct atcttcagcc tctttaaact tttcaaaggc
     4681 ccagcgagcg aacttgatga ccacatagtc gccccacggt gtgtatttat ccagggttcc
     4741 agccctccaa tagggcaggt ccttcagcgt gtaaccccct gcaagctttg cagaaataag
     4801 ggctatagga aatcctgtgg ctttggaagc aagggctgat gagcgggatg ttctcggatt
     4861 gatttcgata acaacaatcc gtccggtatt cgggttgtgg gcaaactgca catttgttcc
     4921 accgatcacc tgaattgcct ctacaatgcg gtaggcgtat tcctgcaatc tcttctgaac
     4981 ctcttccgag atggtgagca ttggtgctga acagaaggag tcgcctgtat gaacccccat
     5041 ggggtcaatg ttttcaataa agcagaccgt tatcatgtta ttgtctgcat cccgaacaac
     5101 ttccagctcc aactcttccc agcctatgac agcctcttca ataagtatct gtccgatgag
     5161 actcgcagct ataccacggc tggcaattgt tttgagctct tccttgttat aagtgattcc
     5221 tccgccccac cctccgagag tataggcagg tcttacaacc accgggtacc ccagtttgtc
     5281 tgcaatggcc agtgcctctt cgatggaata ggcagggtct gacaggggtg tgggtatgcc
     5341 gagcttattc atggtgtttt tgaattcgat gcggtcttcg ccccgttcta tgacatctgc
     5401 cttgacaccg attatctgta ccttatgttt cgccaggatt cctttctttg aaagttcagc
     5461 agccaggtta aggcctgttt gcccacccag attgggaagc agtgcgtcag gtttttcctt
     5521 ttctattatc ttttcaaggg cttccactgt taggggttcc acgtacgtgg catcggccat
     5581 gccgggatca gtcatgatcg ttgcaggatt tgaattcacc aggataacct catagccaat
     5641 agatttgagg gctttgcacg cctgtgttcc ggaataatcg aattcgcacg cctgaccgat
     5701 gatgattggg cctgacccta tgatcagaac cctattgatg cctggaatct tcttcataaa
     5761 taaaattcct tattttggag atttttgctg aagcatccta acagagcccc cctgtgttgt
     5821 caagtatttt cacgatctta gcttgaatag gcgggcctct tgtgatagca taacggaaga
     5881 ggtgattgat gatgaatgga gtaaatgaga atactacaga ggtaatcggg caaggatgta
     5941 aaacgcaagg ttcaaggctg ggccttgtac gtgatctttt tattttttgg ttcgtcatct
     6001 ttcttgtagt tctctgttgg acaaacaaca tcctggtaac agcaatcctc atagctgcct
     6061 atggtgtacg atactatttc tggccgaata aggaagatca cgttttgtat attggtgggg
     6121 caattcttgg ccctatcgct gaagttattg ccaccaaggt ggggatctgg caatataccc
     6181 tgccaacctt tcttaatata cctttgtggc ttccctttgc ctggggcctt gcagcagttc
     6241 tcatcataag aattgccctg gcttttgcga gaaagtaaaa acctttgcat gctttatggg
     6301 aaaattctct tgacaaaagg ccgggatgat tataactttg gtatactttt tttaatcttt
     6361 ttttaaaggc actactatac agtgattaaa tcgttaaaaa tcagatttat tctggtaggg
     6421 ttgtttcttg taatatcaat aatctactgc ctgccaaatg tgtttgaacc aaagggtaac
     6481 ctgaagaaat acctgccttc agacaagata catcttggcc ttgaccttaa gggggggatg
     6541 catcttcttc tcgagcttga tatggccaaa atgatgcaga atctgtcgga gaggaagttt
     6601 accgctctaa aggatgcaat gatcagagat ggagtccggt ttctcgccct tgacatgaag
     6661 gggaacaccg tttcagtggc tgttaaggcg gagcagaaag aaaagcttta caaccttgta
     6721 ggtaaagaat ttcccgatat aaaagcagga acaacaaaga cagagggcga cacattcttt
     6781 gtcgattttg tttttagcga taaagaactc acaagcctca aagacaatgc agtcagacag
     6841 gctcttgaaa ccattagaaa cagaattgac cagtttggtg tttctgagcc tgtgatcgtt
     6901 aaacaaggcg agaatcagat acttgttcag cttcccggta ttgaagatcc tcaaagggcc
     6961 ctcgatctta tcggcagaac tgcccagctt gaattcaaac tggtagatga ggataatgca
     7021 ggcagggcct catcagggag tgtccctgat ggtgacgagc ttctgatgat gcgcacgaag
     7081 aatagagaga ccggaattgc aacgacaact ccaattcttc tgaaaaagca ggctctcctt
     7141 actggcgaac tcttaactga tgcaaaggtc agtattggcg gtgaatttag aaatgagccg
     7201 tatgtaggga tagagtttaa cagtgaaggt gccaggatat ttgacaagat tacagcagaa
     7261 aatgtcggaa aaaggctggc cattgttctt gacaacactg tttattccgc gccggtggta
     7321 aaagagagaa tatccggtgg cagggcgtcc atcacaggtg gttttaccat ggaggaggca
     7381 aaagacctgg ccattgttct gagggccggt gcacttccgg cgccagtcaa ggtggttcag
     7441 aatattacta tcggcccgac ccttggtcag gactccatca gaaagggtat cagggctacc
     7501 atactcggag ccttgttgac tatcattttt atgatcgtgt attaccgggg atgtggtgta
     7561 atagcaaaca tcgccct
//
LOCUS       DAOOMC010000132         7491 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdus sp. isolate AMR_MDS_2685
            NODE_11651_length_7491_cov_5.580689, whole genome shotgun sequence.
ACCESSION   DAOOMC010000132 DAOOMC010000000
VERSION     DAOOMC010000132.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864760
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdus sp. (wastewater metagenome)
  ORGANISM  Syntrophorhabdus sp.
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae; Syntrophorhabdus.
REFERENCE   1  (bases 1 to 7491)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7491)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,729
            Genes (coding)                    :: 2,708
            CDSs (with protein)               :: 2,708
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 7 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7491
                     /organism="Syntrophorhabdus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11651_length_7491_cov_5.580689"
                     /isolate="AMR_MDS_2685"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2699756"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(6..358)
                     /gene="ssrA"
                     /locus_tag="PK125_11570"
     tmRNA           complement(6..358)
                     /gene="ssrA"
                     /locus_tag="PK125_11570"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(361..807)
                     /gene="smpB"
                     /locus_tag="PK125_11575"
     CDS             complement(361..807)
                     /gene="smpB"
                     /locus_tag="PK125_11575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003564285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HPB38783.1"
                     /translation="MKTICTNKKAYHDFHIDEKFEVGIALTGTEVKSLREGRANLKES
                     YARIKNGELFLVNAHISPYSCGNIYNHEPKRTRKLLMHRREIDKLVGKVKERGFTLVP
                     LSLYFNKRNKAKMELALAKGKTLYDKRESIKKKDEKRVAEREARIR"
     gene            complement(866..3001)
                     /locus_tag="PK125_11580"
     CDS             complement(866..3001)
                     /locus_tag="PK125_11580"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPB38784.1"
                     /translation="MKKIKTKLIFSGFILLIFGVLVYLETNLPFFKKFLPIGENKLII
                     VILNINLLLILLLLFLVSRTLVKTYIEQKRGIWGARLKTKLTITLVLISIIPSFTLYV
                     LSGGFFQISMDKWFGQKIEDTLDDALEFSRFYYEDLFQRHERVGAIIANEIKKKRLLD
                     DPKGLAAYVQKNTTSRIPEYFTIYDDSGHLLQSARRLTPEIEKKFSALARSSLKDNKI
                     RAIEPLKKGELILSGLQIANETGEFRAMLFIGEEIEIAGTKSMQEIAIASRELQKSRP
                     FKKLLKWSFYIPLTLVTLMTVFFSVWVGIKMATEIVTPIEKVKEGVSTIAKGSFDLIN
                     LEDKSQDEIGTLVMAFNAMAKDLKIAKDEIEERRRYMEIILDNVATGIISTDKIGTIQ
                     FINKAAQKILGIENEPWAGASLKNILGDDFKKHMKFFLKEAREVRHGSVTREMRLNLK
                     NDVKYIRASLTTLKDQARKTEGFVIAFDDITYIVRAERLATWREAAKKLTHEIKNPLT
                     PIMLSAERIRRRLLPNSHDQEKEVLENTTSVIIQSVNDIKGIVNELTKLTHSVQSKTM
                     EDLNSIVDETVLLYEHLYQNLTLTCEKNALPPFLVDRDGLKRAIINIITNSIKAIGNN
                     RGAIKITTRHDSDKGFNIIEIADTGKGIPNEEKSKIFDPYFTKDANGMGLGLAIVHSI
                     ILEHHGRIHVEDNYPRGTKFIIELPTIEA"
     gene            complement(3090..3788)
                     /gene="pgeF"
                     /locus_tag="PK125_11585"
     CDS             complement(3090..3788)
                     /gene="pgeF"
                     /locus_tag="PK125_11585"
                     /inference="COORDINATES: protein motif:HMM:TIGR00726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan editing factor PgeF"
                     /protein_id="HPB38785.1"
                     /translation="MTKNADWSFFRVPELEEKGIVHGFCTGGTPWDLLSSKIKHDFLD
                     AFLLKDAVVLTQEHGSDVHVVCNGEMPHSGDGLIILEKNVAGIIKTADCLPVILCDAL
                     YPMVSIVHAGWRGTVKNIVGKAVLSMVEYGSRTQNIVALLGPSIGSCCYEVKGDVCAV
                     FKKCGFPEHIFLRHSNALFLDLKKANEWVLSGYGINEVYNVNLCTQCNGTLFHSYRRG
                     DTGKRQINFVSIKG"
     gene            complement(3886..4824)
                     /locus_tag="PK125_11590"
     CDS             complement(3886..4824)
                     /locus_tag="PK125_11590"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HPB38786.1"
                     /translation="MRNRFYLRSDEQGKRLDVFLTEKLTITRSKVKEIFERGSITIEG
                     RTPKPSLKVKKGLIIEGEIPEEKPITVTPQKISLDILYEDRYILAVNKPKGMVVHPAC
                     GHSEGTLVNAILGYLQKTANGQWAIDEGRRSEHYPQDVRPGIVHRLDKGTTGVIIIAK
                     DLKTQEILSGLFKERSVHKTYRAIVEGIVQRETGTIEENIGRHPVHRKKMAVIKNGGR
                     ESETAYRVLQKLDGYTYVEAYPRTGRTHQIRVHFSYMGHPVVGDEVYGKHARRLTGRP
                     LLHAHTISFQHPRKRLKVVIEAPIPEDMIDFISEHQ"
     gene            complement(4814..6214)
                     /gene="selA"
                     /locus_tag="PK125_11595"
     CDS             complement(4814..6214)
                     /gene="selA"
                     /locus_tag="PK125_11595"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004125 - L-seryl-tRNASec selenium
                     transferase activity [Evidence IEA];
                     GO_process: GO:0016260 - selenocysteine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HPB38787.1"
                     /translation="MPGKELLRKIPKVDEILKNRAWEKLVTDYPEGLAKDALRESLEI
                     LRESIKTGEAVSMPLIEEIILSTRQRLTTLMSPRLKRVINGTGVIIHTNFGRSLLAGA
                     AIEAVKNAASYYSNLEYDVEQGMRGDRYGHSTVILKRLTGAESAMVVNNNAAAVILVL
                     NTLAEGKEVIVSRGELVEIGGSFRIPDVMKKSGAILREVGTTNRTYKEDYERAIGENT
                     GLLMKAHTSNFKVKGFTHAVTSEELVDMGRQFGAPTYFDAGSGLLYPANERLLADEPC
                     IPTELERGLDVISFSGDKLPGAPQAGIILGKKIYINMMKKNPLARALRPDKLTLAGLE
                     STLLLYLDKKTAIQEIPTLRMIHTSDVALKKDAQRIARILRSRCANITVSVIPLYSEV
                     GGGTLPDVVIPSYGVGLRPHLISVEKFERRLRSLSIPIIGRIEKDMYLLDMRTLHKSD
                     REPLISGIETAIGDAE"
     gene            6341..6772
                     /locus_tag="PK125_11600"
     CDS             6341..6772
                     /locus_tag="PK125_11600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB38788.1"
                     /translation="MNKSLVLIAVAMVVCLSHVTVLHAVKANRVSNNKISSCLKSKSC
                     KFIITGTATEEPRMSFLVQEKIWKTLTVLDRNDLKRILKARIAEANEKPDKYVHLSKK
                     APSYDRLRKNIENMQSYSVFLSYGKDSKGNLQLDEEILVNY"
     gene            6897..7061
                     /locus_tag="PK125_11605"
     CDS             6897..7061
                     /locus_tag="PK125_11605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB38789.1"
                     /translation="MDTKNTDHTKHRLDGERAKKIIHILIGSKFYFDLNLVERHDLIR
                     YILRRFPFSF"
BASE COUNT         1977 a         1724 c         1434 g         2356 t
ORIGIN      
        1 tattttggtg gaggcggcgg gaatcgaacc cgcgtccaaa gaagaagaat acagaaagac
       61 tacatgctta gtcggtatat taatctcaga tcagagatca cctaccgaca ggttatcatt
      121 gatcccagcc cattcagatt caccctcctg catgggcgtc cagaagggct atcccgcttc
      181 tatgacgcca tgttggactc aaagcgggca gagagtccag atgacgtggc ggctctaggc
      241 tgccagtgcg tactcgtgag agtctgcagt tatgtttagt tccacttgtt ttcgggtaag
      301 tagtaacccc ggcatgcctc cctggccctt aacttccctg tcgagaccta tcgccccctt
      361 tcatcgtatt cttgcctcgc gctctgctac ccgcttttca tcctttttct taatcgattc
      421 ccttttatcg tagagcgtct tgcctttggc gagggcaagt tccatctttg ctttgttcct
      481 cttgttgaag taaagtgaca acggcacaag tgtaaaaccc cgttccttca cttttcccac
      541 tagtttatca atttcccgtc tgtgcatgag gagtttccgg gtcctcttgg gttcatgatt
      601 atatatattt ccgcatgaat aaggacttat atgggcattc accaaaaaaa gctccccatt
      661 tttgatcctt gcatagctct ccttcaagtt tgccctgccc tcacggagcg acttcacttc
      721 tgttcctgta agggctattc cgacctcaaa tttctcatca atatgaaaat cgtgatatgc
      781 tttcttattc gtacaaatcg tcttcatcac actacactaa tatagcacat ttcatgccaa
      841 atttaaacag acacaggact ccttctcacg cttctatagt agggagttct attatgaatt
      901 ttgtgcctcg cgggtagttg tcttctacat gaatcctgcc atgatgctcc agtataatgg
      961 agtgaactat ggcaaggcca agccccattc cattggcatc tttagtaaaa tatgggtcaa
     1021 atatcttgct tttctcttcg tttgggatgc ctttgcctgt atcagctatc tctatgatat
     1081 taaatccttt atcactgtca tgccgcgtgg tgattttgat cgcccctcga ttgtttccta
     1141 tggctttgat cgaatttgta atgatattga taattgcccg tttgagaccg tctctatcca
     1201 caaggaatgg aggcaaagca tttttttcac acgtgagggt taaattctga taaagatgtt
     1261 catacagcaa aacagtttca tcaacaatac tgttcagatc ctccatagtc ttactctgca
     1321 cgctatgggt cagcttggtg agttcgttga ctattccttt tatatcattg acagactgta
     1381 ttattactga ggtggtgttt tcaagaactt ccttttcttg gtcatgcgaa tttggcagca
     1441 atctgcgtcg tatcctctcc gctgacagca taataggagt caaaggattt ttaatttcat
     1501 gggtcaattt cttggcagct tccctccatg tagctaatcg ctcagcccga acaatatagg
     1561 ttatgtcatc gaaagcaata acaaaacctt ccgtttttct ggcctgatcc tttaaggttg
     1621 ttagagatgc acggatatac tttacatcat tcttcaagtt cagcctcatt tcacgtgtta
     1681 cgctgccatg tcgaacctcc cttgcctctt tcaaaaaaaa cttcatatgt tttttgaaat
     1741 catcacccag aatatttttg agggatgccc ctgcccatgg ctcattttca ataccaagaa
     1801 tcttttgtgc ggccttgttt ataaactgta tggttccaat tttgtctgta gagattatac
     1861 ctgttgccac attatcaagg ataatctcca tgtaacgccg tctctcttca atctcatcct
     1921 tggcgatttt caggtctttt gccattgcgt tgaaagccat aacaagtgtt ccaatctcat
     1981 cctggctttt atcttcaaga ttgatcagat caaatgagcc ttttgctatt gtggatacac
     2041 cctctttaac cttctctata ggggttacaa tctctgttgc cattttgata cctacccaaa
     2101 cagaaaaaaa tacggtcatt aacgtaacaa gggtgagagg aatatagaaa ctccatttca
     2161 gtagcttttt aaagggcctt gatttctgca actccctgct ggcaatagca atctcctgca
     2221 tgcttttggt tccggctatt tcaatttcct caccaataaa gagcattgcc ctgaattccc
     2281 ctgtttcatt ggcaatctgc agcccagaca atataagctc accttttttg aggggttcaa
     2341 tggctcggat tttattatcc tttagcgacg agcgtgctaa ggcgctaaat ttcttttcaa
     2401 tttccggtgt aagcctcctg gcgctctgaa gaaggtgtcc tgaatcatca tatatggtaa
     2461 aatactcggg tatccgagaa gtggtatttt tttgcacata agctgccagc cctttgggat
     2521 cgtccaacag cctttttttc ttgatctcgt tagcaataat agcccctact ctttcatgtc
     2581 tttggaatag atcctcataa taaaaacgag aaaattcaag ggcatcatcc aaagtgtctt
     2641 ctatcttttg accgaaccat ttatccatac ttatttgaaa gaatccccca gaaagcacgt
     2701 aaagggtgaa ggaaggtatt atagaaataa gaactagggt gattgttaac tttgtcttca
     2761 gccttgcgcc ccagatacct cttttctgct caatatacgt cttgaccagg gtcctcgaaa
     2821 ctaaaaataa tagaagaagg atcaagagaa gattgatatt caggatgaca atgatgagct
     2881 tgttttcccc aatgggaagg aactttttaa aaaaaggcag gtttgtttcg agatacacaa
     2941 ggactccgaa tatcaacaga atgaaaccag aaaagatgag ttttgttttg atttttttca
     3001 ttatgctgat gcgtggagca taaccatggg cgacaaaaat atttgttttt ataccctaat
     3061 aaattatgag ttcttggtta aagtataccc tatcctttga tagaaacaaa gtttatctgc
     3121 ctttttcctg tgtctcccct cctgtaggaa tgaaacaggg tgccattgca ctgtgtacac
     3181 aggtttacat tataaacctc gttgatgccg tatccagaaa gcacccattc atttgccttc
     3241 ttcaggtcaa gaaatagggc gttgctgtgc ctgagaaaga tatgttccgg aaatccacat
     3301 tttttaaaaa ccgcacagac atccccttta acctcgtaac agcacgaccc tattgatggg
     3361 ccaagaaggg caacaatatt ttgtgttctg ctgccatatt ccaccatgga taagactgct
     3421 ttgcctacaa tattttttac cgttcccctc catcctgcat ggacaataga taccatggga
     3481 tacagagcat cacacagtat gacagggagg caatcagccg ttttgatgat gccagcaaca
     3541 tttttttcaa gaataatcag tccatcacct gaatgcggca tctccccatt gcataccaca
     3601 tggacatcac tgccatgttc ttgggtcaaa actacagcat cttttaataa aaaagcatcc
     3661 aaaaaatcat gctttatctt actgcttaga agatcccatg gagtgcctcc tgtacaaaac
     3721 ccgtggacaa tccctttttc ttcgagttct ggcactctga aaaatgacca gtcagcattt
     3781 tttgtcatct catacttcat gattcactat tttattgtgt ccttctaatt atatctttct
     3841 ccacttatct cacggtctat atctatctca tttatacttt tttgcttact ggtgctctga
     3901 aataaaatcg atcatatctt cgggaatggg cgcttcgatt actaccttaa gccttttcct
     3961 cggatgttga aaagagatag tgtgcgcatg tagtaaaggt cttccggtta agcgtctagc
     4021 gtgtttccca tagacttcat cacccaccac cgggtgaccc atgtacgaaa aatgaacacg
     4081 aatctgatgg gttctacctg ttcttgggta tgcctcaaca tatgtatagc catcaagttt
     4141 ttgaagaacc ctgtatgccg tctctgattc tctgcctcca tttttaataa cagccatttt
     4201 ttttctgtgt accgggtgtc ttccaatgtt ttcttcaatg gtccctgttt ctctctgcac
     4261 tatcccttcc acaattgctc ggtatgtttt atgaacgctc cgttctttaa aaaggccaga
     4321 cagtatttcc tgggtcttga gatctttagc tatgatgatc acgccagttg ttcctttgtc
     4381 aagtcgatgg acaataccgg ggcgcacatc ttgcgggtag tgttcagagc gccttccttc
     4441 atctatcgcc cattgtccat tggccgtctt ttgcaggtat cctaaaattg catttacaag
     4501 agtcccctcg ctgtggccgc aagcaggatg cacaaccatc ccctttggtt tgttcacggc
     4561 aagaatgtat ctatcttcat agagaatatc gagagatatt ttctgtggtg taactgttat
     4621 aggcttttct tcgggaatct ctccttcaat gattagacct ttcttcactt taagagacgg
     4681 tttcggggtt cttccctcga tcgtgatact tcccctttca aaaatctctt taaccttact
     4741 tctggtgatg gtgagttttt ctgtgaggaa tacatcaagg cgtttcccct gctcatcaga
     4801 tcggaggtaa aatctattcc gcatctccga tagcagtttc tatgcctgaa atcaaaggtt
     4861 ctctgtcgct cttgtgtaaa gttctcatat caagaaggta catgtccttt tcaatcctgc
     4921 cgatgattgg gatcgaaaga ctgcgaagtc ttctctcgaa cttttctaca gatatcaggt
     4981 gtggcctcaa gccgactcca taagagggaa ttacaacgtc aggaagggtt ccaccgccaa
     5041 cttcagaata tagcggaatt acagaaacgg taatattagc acatcgggac cgtaaaattc
     5101 ttgcgatcct ctgtgcatct ttcttcaatg caacgtcact ggtatggatc atacgcaacg
     5161 ttggtatctc ttggattgca gttttcttgt caagatagag aaggagcgta ctttcgaggc
     5221 ctgcaagggt aagtttatct ggtcgcaagg cacgagcgag agggttcttc ttcatcatgt
     5281 tgatatatat ttttttccca agtattattc cggcctgggg ggctccagga agtttatccc
     5341 cgctgaaaga tatcacgtca aggcctctct ccagctctgt tggtatgcat ggctcatcag
     5401 caagcaatct ttcattcgcc ggatacagca aaccacttcc cgcatcgaaa tacgtgggag
     5461 ccccaaattg tcttcccatg tctaccagct cctctgacgt tactgcatgg gtgaaacctt
     5521 tgaccttgaa attgctcgtg tgagccttca tcagaagtcc tgtgttttct cctattgccc
     5581 tttcatagtc ttctttgtat gtcctgtttg ttgttcccac ttctctcaga attgcaccac
     5641 tttttttcat gacatcagga atccggaaag agcccccaat ctccacgagt tcccccctgg
     5701 agacaataac ctcttttcct tcagcaagtg tgttcaggac gaggattact gcggcagcat
     5761 tgttgttgac caccatagcg ctctcggcac ctgtaagcct cttcaggatt actgtagaat
     5821 gaccatatct atcgcctctc atcccttgct ctacgtcata ttcgaggttt gagtaatacg
     5881 aggctgcgtt tttgacagct tcaatcgctg ccccagcaag gagcgatcta ccaaaattcg
     5941 tatggatgat aacgcctgtc ccattaatca ccctcttcag acgaggactc atcagagtgg
     6001 tgagtctttg tctggtagaa aggattatct cctctatgag aggcattgaa actgcctccc
     6061 ctgtcttaat agattccctc aaaatctcca ggctttcccg gagcgcatcc tttgcaagcc
     6121 cttcgggata atctgtaaca agcttttccc aagcacgatt ttttaagatt tcgtctactt
     6181 tcgggatttt cctcagtaac tctttgccag gcatgtgatt atttttaaca cacaaatcat
     6241 tatataatca attatatatc ccgcaaaatt gtatttgaca tcaactaaaa cgtttgatag
     6301 tattacactt tattgcttct gagcatcatg ggggggtatc atgaacaaat cattagtttt
     6361 gattgctgtg gcaatggttg tctgcctgtc gcatgtcact gttctgcatg cggtcaaggc
     6421 aaatagggtt agcaacaaca aaatatcaag ctgtctgaag tcaaaaagct gcaaatttat
     6481 tattactgga acggctacag aagaaccaag aatgagtttt cttgtccagg aaaaaatttg
     6541 gaaaaccctt actgttcttg atagaaatga cctgaagcgg atcttaaagg caagaatagc
     6601 tgaggccaat gagaaaccgg acaagtatgt tcacctatct aagaaagcgc catcctacga
     6661 cagactcaga aaaaatatag aaaatatgca atcatattcg gtctttcttt cctatgggaa
     6721 ggactccaaa ggaaatttac agctcgatga agaaattctg gtgaattatt aaaaaacata
     6781 ttacaacagt ttaagcacta cctcccacag cgtgcaaaca gggccgaatt aacttttcca
     6841 cgaaaatagc cgatttaatc attgacaaca atgaggttta tccaggagca taactcatgg
     6901 acaccaaaaa tacagatcat acaaaacatc gccttgacgg ggaacgggca aagaagatta
     6961 ttcacatact aataggaagc aagttttact ttgatttgaa tcttgtggaa cgacacgatc
     7021 ttattcgtta cattttaaga agatttccat tttctttcta aaaacaaatc ctacaaagtt
     7081 tgtagctttc tagctccttt tgtagccaat attacaacac aaggagttgc tttgttaaat
     7141 gaccttaacc caattcaata aatacgttca taatatgcaa ataatatggg ctttcattaa
     7201 aaaactctgt cattcaccct gagtacgtga ggcctggttc cgatggtata gattttgctg
     7261 ttcaagaggc atgacagagc gtacgaaagt aaaaaaaata attcgaatac tgattggtag
     7321 caggttctat ttcgatcttt cccttcagga aagatacgat cttataaggt acatactcag
     7381 aaaattccct ttttctatat gaggcttccg aattttctgt aagcagccca taacttgtta
     7441 tcctttgtgc ggggccctgt aaccttcagc atgcattatc agaaagccta g
//
LOCUS       DAOOMC010000133         7408 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdus sp. isolate AMR_MDS_2685
            NODE_11844_length_7408_cov_7.067999, whole genome shotgun sequence.
ACCESSION   DAOOMC010000133 DAOOMC010000000
VERSION     DAOOMC010000133.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864760
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdus sp. (wastewater metagenome)
  ORGANISM  Syntrophorhabdus sp.
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae; Syntrophorhabdus.
REFERENCE   1  (bases 1 to 7408)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7408)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,729
            Genes (coding)                    :: 2,708
            CDSs (with protein)               :: 2,708
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 7 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7408
                     /organism="Syntrophorhabdus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11844_length_7408_cov_7.067999"
                     /isolate="AMR_MDS_2685"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2699756"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..408)
                     /locus_tag="PK125_11610"
     CDS             complement(<1..408)
                     /locus_tag="PK125_11610"
                     /inference="COORDINATES: protein motif:HMM:NF023853.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase III"
                     /protein_id="HPB38790.1"
                     /translation="MELTELFGLNVFNDKVMKQRLPKETYRALKETIEKDMPLKPDVA
                     NVVANVMKNWAIEKGATHYTHWFQPLTGITAEKHDSFISPTDDGGIVTKFSGEQLIHG
                     EPDASSFPSGGLRSTFEARGYTAWDCTSPAFLKT"
     gene            776..1633
                     /locus_tag="PK125_11615"
     CDS             776..1633
                     /locus_tag="PK125_11615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB38791.1"
                     /translation="MKILVFPIILGLCIFVVFPAQAQLVVYDDFSGPFVDIARWYHRH
                     SGTSDNVWPFEGGFRIQKGKLNYFSRAYGTKLATQETQDYRRRLFLKDVSGVTAIETS
                     IQMAKNGNVITGCSENTKPSETRVNFGGSFFNNGSGLEPNNGDYTGDVYATIALYKFS
                     DSTDIPAGYMGVRARVRLCTNEDCSMSSQLYETFFKDDRDKPLLLKVGKKEKFRITYD
                     RAANAFTFQVGKNVATYTSTVENVQPSGALNGGMRLAVDHSLANCPTSNRAIGWADAF
                     FDYISIRQE"
     gene            1858..2046
                     /locus_tag="PK125_11620"
     CDS             1858..2046
                     /locus_tag="PK125_11620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB38792.1"
                     /translation="MHDILPEGEDLRRAIKWISGSIQENTRASVSQLVEEATFKFDLS
                     PKDSEFLMNFFRNRKEGQ"
     gene            complement(2062..2979)
                     /locus_tag="PK125_11625"
     CDS             complement(2062..2979)
                     /locus_tag="PK125_11625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HPB38793.1"
                     /translation="MRLLTRSDFDGLICAVLLKHIGIMDEWKFVHPKDVQDGLLEVTT
                     DDILANVPYVPGVGMWFDHHSSEQERLRFNYKYEGASELKPSCARVIWDYYGGKENFP
                     ELFQEMMEYVDKCDSGNLTHQEVLDPKGWVLLSFIMDPRTGLGRYKDYRISNYNLMMK
                     MVEWCGTLPIDEILAKPDVQERVERYERQNELFRQQLTKCTVLHKNLAVIDLRSEDEI
                     YTGNRFLVYSLFPSCNISMHVMWGREKQNVAFTIGHSIINRTSKTDVGKLMLEYDGGG
                     HKRVGTCQIATPRAERIKQILIDRITGDG"
     gene            complement(3153..3959)
                     /locus_tag="PK125_11630"
     CDS             complement(3153..3959)
                     /locus_tag="PK125_11630"
                     /inference="COORDINATES: protein motif:HMM:NF024634.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HPB38794.1"
                     /translation="MILNRPVRLLYFSPTGTTRKILEKIAEGIGEDQPEHIDLTSPEA
                     RMGQHSRIKRGLTIIGVPVYTGRVVLEAIRGLGQFKATDAPAIVVVVYGNREYEDALL
                     ELQNITVEAGFTPIAGAAFIGEHSLSTKDVPVAHGRPDSEDLDCAAAFGVLVNEKLTK
                     TDSLSSVSPLKIPGNFPYRERGPARHVSPDSDDSTCTRCEKCREVCPTAAITLVEGYI
                     QTEKNKCILCCACVKSCPYGARHLNDPQLRQFIQQLSITCSMRKDPEFYL"
     gene            4122..4544
                     /locus_tag="PK125_11635"
     CDS             4122..4544
                     /locus_tag="PK125_11635"
                     /inference="COORDINATES: protein motif:HMM:TIGR02481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin family protein"
                     /protein_id="HPB38795.1"
                     /translation="MPVKFLWDLRYSVGDEVIDSQHRRLFDLGNEMQNIQLPEVARTI
                     MNLYRHTREHFDTEEQHMKVIGYHELDQHRELHNGLISGLNSLIEKPVDSVAGLERLK
                     KFVYDWIIDHILNHDKKYFEFYQEKIKRNVTISKEISQ"
     gene            4753..6147
                     /locus_tag="PK125_11640"
     CDS             4753..6147
                     /locus_tag="PK125_11640"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HPB38796.1"
                     /translation="MILERFENLNLSKELHRAVRELEFDEMTPIQAKTIPLILEGRDV
                     IGQAQTGTGKTLAFGLPILESIHPRAKKPQVIVLCPTRELAIQVAAELQSVLKYRKEI
                     IILPVYGGQPIDRQIRTLNAGAHVVIGTPGRTIDHIKRGTLKLENVRTVVLDEADEML
                     NMGFIDDVEQILQHVPQERQTLLFSATMPRPILNLTRKYQSDPEFVKVVHQQLTVPNV
                     EQSYFEVREASKLDVLCRVIDMYGLKSSLVFCNMKRRVDEVVMQLEARGYPAQGLHGD
                     MTQPQRNHAMERFKRNRTEILVATDVAARGLDIENIEAVFNYDLPQDEEYYVHRIGRT
                     ARAGRNGQAFSFVVGRELYKLHEIESYASTKIHRKPVPSIADIEEVKVTSFLERVKGV
                     VEKGGLERYENLMEQLLKEDYSSLDIASALLKITMDEDVKEYMKETDIMVEQDRRRKK
                     ANVGRRSFSPRRRR"
     gene            6573..6782
                     /locus_tag="PK125_11645"
     CDS             6573..6782
                     /locus_tag="PK125_11645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB38797.1"
                     /translation="MCGLLIKPLIISISFILLWFGAKGLREDQVIEEGNEIIGREKSP
                     ANYWMTVIIYIGGGIAGLLFSLICL"
BASE COUNT         2139 a         1547 c         1638 g         2084 t
ORIGIN      
        1 agtcttgaga aaggctggag acgtacaatc ccaggcagtg taacctctcg cctcaaatgt
       61 gcttcgaaga ccgccgcttg gaaatgaaga tgcatcaggc tccccgtgta tcagttgttc
      121 gccagagaat ttggtcacta tgcctccatc atcagtaggg gagatgaaag aatcgtgttt
      181 ctcggctgtt atccctgtta atggctggaa ccagtgtgta tagtgggttg cccctttttc
      241 tatagcccag ttcttcatca cgtttgcgac tacatttgca acatctggtt tcagaggcat
      301 gtctttttcg attgtctcct tgagtgccct gtatgtctcc ttgggcaacc tttgcttcat
      361 cactttatcg ttaaaaacat ttaatccaaa aagttcagta agttccataa tttatctcca
      421 tatagcagag catcctttaa aattgaccta cttttggtat cgcctgtgtg tcacaataca
      481 cttttctcat attagtttag tccacgagga caacgcttgt gaacctcttt gttcaatttc
      541 tgtgaaaagc ctcttgagct ttgatgggtt tggcacgccg gtgtgctcaa agattttaat
      601 ataaagcgcc ttttattaca acttctttta cgtcaaaaat tcattgtaag ctgacactcc
      661 acaaggccag ccacacaaaa ttttctgcat aaattgaaaa cagctgctat aattagttta
      721 agtaggtctt tcactgatgt gcttcaataa aatcatacat aaggagggaa caaatatgaa
      781 gattttagtc tttccgatta ttcttggttt gtgtattttt gtcgtattcc ctgcccaggc
      841 acagctcgtt gtgtacgatg acttttcagg cccatttgtt gatattgccc gatggtatca
      901 tagacacagt ggaaccagcg ataacgtttg gccgtttgaa ggcggtttcc ggattcaaaa
      961 aggaaagctc aattatttca gtcgtgccta tggtactaaa ttggcaacgc aggagactca
     1021 agattacaga cgtcgacttt tcctaaaaga tgtttcaggt gtgactgcta ttgaaacatc
     1081 cattcagatg gcgaagaatg ggaatgtaat caccgggtgc tcagaaaaca caaaacccag
     1141 cgagaccaga gtcaattttg gtggaagttt ttttaacaat ggaagtggtc tggagcccaa
     1201 caatggagat tatacaggag atgtctatgc tacaattgca ttgtacaagt tttcggattc
     1261 tacggacatt cctgctggct atatgggagt aagggccagg gtgcgcttgt gcacaaacga
     1321 agactgcagt atgtctagcc agctctatga gacgttcttt aaggatgaca gagacaaacc
     1381 tttacttctc aaggtaggga agaaggaaaa atttcgtatc acatacgacc gtgctgccaa
     1441 tgcgttcact ttccaagtag gcaaaaatgt agctacgtat acgagcacgg tagaaaacgt
     1501 acagccctcc ggggccctca acggaggtat gagactcgca gtagaccact ccctggcaaa
     1561 ctgcccgaca tcaaatcgtg caattggatg ggccgatgct ttttttgact atatctctat
     1621 tcgtcaggaa taacaggctg gtaggtgtga tagtataagg aggcgctttc ttcccgcaag
     1681 gggttggagg ggcctttttt atggttgggg ccaagccaat tattggattg caccggcagt
     1741 gttatatgta tattcatctg gttgatatcg gcgcttgcag gatacaggtt tttatactat
     1801 agttttattg taccgagatg ctatcatttc gaaaaaagag tgggagtgag acctatcatg
     1861 cacgatatat taccggaagg ggaagatctg agaagagcga tcaaatggat atcaggcagt
     1921 attcaggaga ataccagggc atcggtttcc caactcgttg aggaggcaac cttcaagttt
     1981 gatctttccc cgaaagattc tgagtttttg atgaactttt ttcgtaatag gaaagaaggg
     2041 caatagaacg ccatcaacac atcatccgtc gcctgttatg cgatcgatca atatctgttt
     2101 aatccgttcc gccctcggag tggctatctg acaggtgcca acacgtttgt gccctccacc
     2161 gtcatattct aacatgagtt ttcccacgtc cgttttgctt gtgcggttga tgatactgtg
     2221 tccaatcgtg aaagcgacat tttgtttttc ccttccccac atcacatgca ttgaaatatt
     2281 acacgaggga aacaggctgt agacaaggaa acggttgcca gtataaattt catcttcgct
     2341 cctgagatca ataactgcga ggtttttatg cagaacagta cattttgtga gctgctgacg
     2401 gaacagttca ttctgtctct catagcgctc cactcgttcc tgtacatcag gttttgccaa
     2461 tatttcatct attggcagcg taccacacca ctcgaccatt ttcatcatca ggttatagtt
     2521 gcttatccga taatccttat aacgccccag gccggtccgt gggtccatta tgaatgagag
     2581 caacacccag cctttcggat ccaaaacctc ttggtgcgtt aagttgccac tgtcgcattt
     2641 gtcaacatac tccatcattt cctgaaacag ttctggaaaa ttttcttttc ccccataata
     2701 gtcccagata acccgcgcgc aactcggttt cagctcgctt gccccttcat atttgtaatt
     2761 aaacctgagc cgttcctgct cgctggaatg gtgatcaaac cacattccaa cccctggaac
     2821 ataaggcacg ttggcaagga tatcatctgt ggtaacctcg agaagcccat cctgaacatc
     2881 cttaggatga acaaacttcc attcatccat gataccaata tgtttaagaa gaactgcaca
     2941 gataagaccg tcaaaatcag atcttgtcaa aagcctcata taaacacccc ttggtttatg
     3001 tcaatgattt gtcgaaattg gaacttaact catcttctaa atattggtcc caaattcaac
     3061 ccatagattg tatcggttat ttaaaaaaga tgttaaatgg atataatgta agtaaaatcc
     3121 attactgggc tactccatcc gggaaaattt atttagagat aaaattccgg atctttacgc
     3181 atgctacatg tgatgcttaa ttgttgaatg aactgtctga gctgtgggtc gttcaagtgt
     3241 cttgccccgt aagggcagct tttgacacag gcacaacaga gaatacattt attcttttcg
     3301 gtttgtatat agccctcaac caatgtaatg gctgcggtgg gacagacttc ccggcatttt
     3361 tcacatcgtg tgcaggtaga atcatcgcta tctggagaaa catggcgtgc tggccccctc
     3421 tccctgtatg gaaaatttcc agggattttg agtggtgaaa cactgcttaa ggaatctgtt
     3481 tttgtcagct tctcattcac caatactcca aatgctgcgg cacaatcgag atcttctgaa
     3541 tctggccttc catgagctac agggacgtct tttgttgaga gagagtgctc accaatgaaa
     3601 gccgctcctg caatgggtgt gaaacctgct tcaacagtta tattctgtaa ttcgagaaga
     3661 gcatcttcat actccctgtt tccatatacg acaaccacga tagctggcgc atcagttgct
     3721 ttgaactgcc cgagtccacg aattgcctcc aggacaaccc tgccagtgta cacgggaact
     3781 ccgataatcg taagccccct ctttattctt gagtgttgtc ccattcttgc ctcgggtgat
     3841 gtgagatcga tgtgctccgg ttggtcttca ccaatgcctt ctgctatttt ttcaagaatt
     3901 ttccgtgtcg ttcccgttgg tgagaaataa agaagtctga caggtctgtt caatatcatg
     3961 ataagctccc atttttagct ttattttacc gcgttaagag agttgtttcc agacacaatt
     4021 ctgaaacctt gtttaccata aaatgaattg agattcttaa atcatcagtg aactttccga
     4081 aaacaataga aattataaat gcataccttc aggaggacat tatgccagta aagtttttat
     4141 gggatctgcg atattcagtc ggtgatgagg taattgattc tcaacatcgc cgcttattcg
     4201 accttggtaa tgaaatgcag aatatacagc ttccagaggt tgcgaggacg attatgaatt
     4261 tatacaggca tacccgagag cactttgata cagaagagca acatatgaag gttataggtt
     4321 atcatgaact tgatcagcac agggagttgc ataatggact tatcagcgga ttgaatagtc
     4381 ttatagagaa accggttgat tctgttgcgg ggcttgaaag actgaaaaag tttgtatacg
     4441 actggattat tgaccatatt ttgaaccacg acaagaaata ttttgaattc tatcaggaaa
     4501 aaataaagcg aaatgttact atttcgaagg aaataagcca atgaagcctg gaaaatcagt
     4561 gacacgatca ggtaaattgg ctgcctgatg ttttcaatac aggtctatct gacatatcat
     4621 ttctttgata tattggctga ataatgctat actccaacgt ttcgtgagtt tctgtccgcc
     4681 attttcactg catttcactg catacgtgca agctaattca cggttacaat cataaaaata
     4741 aaaaggaata tcatgatatt agaaagattt gaaaatttga acttgtcgaa agaactgcac
     4801 agggctgtcc gtgaacttga atttgacgag atgaccccta ttcaggcaaa gaccatcccg
     4861 ttgattcttg aaggcagaga cgtcattggc caggcacaaa ccgggacagg gaaaaccctt
     4921 gcttttggac tgccaatttt ggaatcgatc caccccagag caaaaaaacc acaggtaatc
     4981 gtcctgtgtc ctacccgcga actcgcaata caggttgctg cagaattgca gagtgtcctc
     5041 aaatatagaa aagaaattat tattctccct gtttatggtg gccaacctat agatcgccag
     5101 attcggaccc tgaatgccgg tgctcatgtg gtaattggaa ctccaggccg aacgatcgac
     5161 catataaagc gaggaacctt aaagttggaa aatgtaagga cggttgttct tgacgaggca
     5221 gatgagatgc tcaatatggg ttttatagat gatgtagaac agattttaca gcatgttcca
     5281 caggagagac aaacgcttct cttttcagcc acaatgccaa gaccaatact gaacttgact
     5341 cgtaagtacc agagtgatcc cgagtttgta aaggtagtcc atcaacaact gacagtacca
     5401 aatgtggagc aaagctactt tgaagtccgg gaagcatcta agcttgatgt attatgtcgt
     5461 gtcatagata tgtatgggct gaaatcctcc ctcgtgtttt gtaatatgaa aagaagagtg
     5521 gatgaagttg ttatgcaact tgaggcaagg ggttatccag cccagggact tcacggagat
     5581 atgacgcagc cccagcgaaa ccatgccatg gaacgattca agcgaaaccg aacggaaata
     5641 cttgttgcga ctgatgttgc tgccagaggg cttgatatcg aaaatatcga ggctgttttt
     5701 aattatgacc ttcctcagga tgaggagtat tacgttcacc gtataggtag gactgcccgg
     5761 gccggaagaa atggccaggc gttttccttt gtggtgggga gagagctata caaattgcac
     5821 gagattgaat cctatgcctc aacaaaaatc catcgtaaac ccgtcccttc cattgctgac
     5881 atcgaggagg tgaaggtcac atcctttctg gagagagtca aaggggttgt tgaaaagggt
     5941 ggtctcgaac gctatgaaaa tctgatggaa caactcttga aagaagacta ttcatcgcta
     6001 gatattgcgt cagccttgct caaaatcacc atggatgaag atgtgaagga atacatgaag
     6061 gagacagata tcatggtaga gcaggataga agaagaaaaa aggcaaatgt gggaaggagg
     6121 tcattctcac caaggcgaag acgttagcac gactgaaggg caatcccaga tcacaagtag
     6181 ttgcatttat ctctgtacac tattaacgag cgccagcacc gaaaaaactc gttaccctac
     6241 caaatgtttt caccactaat ctacccatag ttctctaagc tcgctttcta aatcaagtcc
     6301 attaagtaaa gagtgattgg cggcgatata tcataaaggt agactaatca tgtgcaactc
     6361 aacagcaaca ttcattgttt ttgtcacgag ttccatcctg ttatggtatg gctacacagg
     6421 actcaggagc aacagggtct gcgttaaagg tggatactac attgatcggt ttgtatcgcc
     6481 aatcaactac tggcttaacg tgggggtata cttagttgtg ggtatcggag gtctttgttc
     6541 cagtcttttg atgatatgga tgagataaaa atatgtgcgg attgctcata aaacctttaa
     6601 tcatttccat aagctttatc ctcctgtggt ttggtgctaa gggactccgc gaagaccagg
     6661 ttattgaaga aggcaatgaa ataatcggac gggagaagtc accggcaaat tactggatga
     6721 cagtgattat ctacatagga ggtggtattg ccggacttct tttttccctt atctgcctct
     6781 aacttaaccc gggaaacacc cattttacat gatcaccaga tgctgaatat gcgaaccatt
     6841 tttgatatag ccataggatt ttaataatta tctttctatt accacgcaaa aacctatatc
     6901 catttctttc atggaagtgt taagaccaaa gcaatagagg atccgtgtag ataatttaat
     6961 gaattgaccc agcatccacg atgcacctcc tgacaaaccg atggtttcct ccatgttgca
     7021 gacacgggaa aaagtgtggg aacttattgg cagacattat tctatcgata ttccgaaaaa
     7081 tatctgaaag gctgggacgt cattccatag aaaattgcgc taatggatga aagaacagaa
     7141 ctcaaaggcc ttccaggaag tggctcataa gagaatatcc aaaaattgct gccatgacaa
     7201 agaagggaaa agcatagatt acgtgggtaa caaaacaggt gaacaaaata aggtttgcag
     7261 ctatgggtaa aaggggaaaa cgaccgatgc tcagcgtggc agtgaagagg ataaatatca
     7321 tcacgaggtt tataatcatg aaagataagg agggaaaggc caggtttatg gactgccggg
     7381 aagcgatgaa ctatagaagg ctcattac
//
LOCUS       DAOOMC010000134         7224 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdus sp. isolate AMR_MDS_2685
            NODE_12239_length_7224_cov_7.326824, whole genome shotgun sequence.
ACCESSION   DAOOMC010000134 DAOOMC010000000
VERSION     DAOOMC010000134.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864760
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdus sp. (wastewater metagenome)
  ORGANISM  Syntrophorhabdus sp.
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae; Syntrophorhabdus.
REFERENCE   1  (bases 1 to 7224)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7224)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,729
            Genes (coding)                    :: 2,708
            CDSs (with protein)               :: 2,708
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 7 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7224
                     /organism="Syntrophorhabdus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12239_length_7224_cov_7.326824"
                     /isolate="AMR_MDS_2685"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2699756"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            356..432
                     /locus_tag="PK125_11650"
     tRNA            356..432
                     /locus_tag="PK125_11650"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:390..392,aa:Arg,seq:tcg)
     gene            complement(463..1029)
                     /locus_tag="PK125_11655"
     CDS             complement(463..1029)
                     /locus_tag="PK125_11655"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013756.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HPB38798.1"
                     /translation="MKKIKVHEGRAYYPMPCAVVGAKIGEKANYLTVAWFSMVNPDPP
                     YLAIAMNKAHYTNLGIKTNRTFSLNIPSVDIAEKVDYCGLVSGKKFDKATIFETFYGE
                     LETAPMVKECPFNVECRLVQTVELPAEDLFIGEIITAYCDENCLTGGTPDLKKINSFI
                     LSMPDKRYRALGQDIGSAWDMGKKHIKK"
     gene            complement(1223..1393)
                     /locus_tag="PK125_11660"
     CDS             complement(1223..1393)
                     /locus_tag="PK125_11660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB38799.1"
                     /translation="MIEFVTITIRKGTNIPAHSIMQSRLQCNIMVIRKKPWDNTKNRY
                     VYLKKIKDCAIA"
     gene            1617..2468
                     /locus_tag="PK125_11665"
     CDS             1617..2468
                     /locus_tag="PK125_11665"
                     /inference="COORDINATES: protein motif:HMM:NF022750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3108 domain-containing protein"
                     /protein_id="HPB38800.1"
                     /translation="MISRRYILKWFFGLPLLSLFPFQISDVRAQATQSGNGKIAASIG
                     EFFAGEELQFEISFMFLKKVAVARMTFKRAEKKGRYISVLEGETVGIVGWLSRYRADT
                     YCTVMEETEGGMRLRSVSFDENVRIGKKTRTFSHEFDYEKKKWTKRSTRRGARLRTVE
                     AAIPEGKVYDDFLCASYNFRYGVYGPLERGRTYIIPTFPRKRSSKYEIKIAAKEKEDH
                     QRVADKVGDEAALLLQLKLDPEITDSKTGIIDGWLSKDNCPLEGIIRDAILFGDVHGK
                     LVSRIKR"
     gene            2678..7132
                     /locus_tag="PK125_11670"
     CDS             2678..7132
                     /locus_tag="PK125_11670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB38801.1"
                     /translation="MLKKGKQKQDKKAANLHKDSSFNIRDLVMRTPASTSPERSPELY
                     KDEKRAKPLKQDTIAKPSPAAAIKSSNDKLAQIIKELSSFDVRTIQRRSDPRMKALCG
                     QINDILAEIFGRNSDEYDDYVIYSLDTLPITIGGSWHPLPEVQEGYRKGIQSAITKLT
                     SLLVIQKSKLEIVSGEKPARNVVNEPSVNQVITGKEKVSLVNLRGKIRSRETVFFSQC
                     EGTENDHRKSHSNSEESDTRARAIKRFSQNNHCKPIQTETEVAMHHTGDEESIQKINP
                     DNNKPVLLENLEEKLKTLESEPVFKDKENEFVETEHDPLFGKPNHEESPLSTLVMQLN
                     EIVTSDPGYTETRLSNVTGSNEKNLSFMQKEGGMCIGFDEESATPSFERVAEIYATDS
                     EISEIEEVLVIDGDDILSTEDSLIQTLENEDREQEIAKSAINDECIDRITDGKKRISL
                     DELQQKLREFEGKEFLQSEPLNDFENKENEEAFLVDGADCLPEEMMKELVQEAGSLDV
                     CEIQPPDREVLIIDGDVDPDQHAFSVDDDEEPHEIEAHESLTQEKNDDEGMLVIECDS
                     GLPEELLNELGQEDSLVCKEDQLQRPLKNVEVRENLTNLSEQYSLDRSMIIDLCSSTA
                     SLNICFLKEDDGTKRETILEDLEKRLTHFSLLGPGEESLHWTEVYDVEKVNSVAAQGQ
                     SDMGTNEDHASSGSPIETEQTYVDLLHSFDVKNVFSAGFDKDFPLKEIAISNEAPEFS
                     DVVGCDENAIMECTDRLLPVCLDVLHCVAVDNILVEDIAFIEQNKKLPDQNIIIEKVC
                     EECSDYISFNNLESLTDHTSEMHITTPVEFFDVERMFEENNDEKHFDGDLTDFCQLQP
                     VEDWVDSNNGDQQEFKFIAPEAMKEEPVPFFMDEELTDQVAEKLCVHNEETFEELPQL
                     IFDPFDGIDNHEFPPITGNVDLTVETAEHHIVDSILMNVNEELLVKPGTDESASDDIS
                     RIIESQITLPVAIKDLMNDSLMIKSDIEIPESLKLEEPFSPRGREEYCTFDINDAFVP
                     SIPDTTIEIHNNALFEPLRHDLSDNEPLPCSIFEECIVCPDASANVECKVDENLRETI
                     LLEALEKMLIDLESDVSEQSHVDFQPITTKCTPLPEEEKTTVLEPQKESPQETVLIKT
                     PEERSNPFAMTGMGREKTNAKMICADDLQVQIGELRYRIDDLRTFDIDSIDQRFDPRV
                     RALGDTVNNTIADIFGRNTPSYWQHALPSLDSLPVVVGGPKLSPEELRGAYRKRINEA
                     IAKVNVTISMLETKLGKLQDKGVNKEHFQPITTKCTPLPEEEKTTVLEPQKESPQETV
                     LIKTPEERSNPFAMTGMGREKTNAKMICADDLQVQIGELRYRIDDLRTFDIDSIDQRF
                     DPRVRALGDTVNNTIADIFGRNTPSYWQHALPSLDSLPVVVGGPKLSPEELRGAYRKR
                     INEAIAKVNVTISMLETKLGKLQDKTPSGQVLFFSQKTNNYKDVQSLH"
BASE COUNT         2330 a         1343 c         1580 g         1971 t
ORIGIN      
        1 aattatagca tattagccag aatattgcac gacaaaagtc cgacaggctc ctagggaaat
       61 tagtacttgg tatatctgta acagatctat ccggccatcg catatcattt ggaagggcaa
      121 cgggaaggta ctttggaaag attatttcag gtataatatt aagctttggt tatataatgg
      181 cggccttcac aaccaaaaaa caggcgcttc atgacatcat ggcatctaca ttggtcatca
      241 aaaattaaat atgggggcgt tagacggttt ttgaagcaaa tgggaagagt actttaggtc
      301 aaaagaccga gtgcctttct ttaacagata tggtattatg aagccatgac aaagcgcgcc
      361 catagctcaa ctggacagag cgttggactt cggatccaaa ggttgagggt tcgacccctt
      421 ctgggcgcgc caatgatgat ttatgattta tatcatcaag gcttattttt tgatatgctt
      481 ttttcccatg tcccatgcgc tgccgatatc ctgaccgagc gctcgatatc tcttgtcagg
      541 catgctgaga ataaaagagt ttatcttttt caagtcggga gtgcctcctg ttaaacagtt
      601 ctcatcgcaa taagcagtga tgatttctcc gatgaaaaga tcttcagctg gtagttcaac
      661 agtctgtacc agtctacact ccacattaaa tgggcattcc ttgaccattg gtgcagtttc
      721 aagctctcca taaaaagttt caaatattgt agccttatca aattttttgc cagaaaccaa
      781 accacaatag tcaacttttt cagcaatatc cacagaaggg atgttcagac tgaaggttct
      841 gttagttttt atgcccaaat ttgtgtaatg agctttgttc atcgcaatag ccaggtatgg
      901 agggtcagga ttcaccatac taaaccatgc gaccgtaagg taatttgctt tttcgccaat
      961 ctttgcccca acaactgcgc agggcatagg atagtatgca cgtccctcat gtaccttaat
     1021 cttcttcata atgcacccct tgagaatgac acaataaatt tacaaatgac tttaatgtgc
     1081 gaagatcgct ttgtcaagaa aaatgagggt tatttccttt gacaggattt cactgcgacc
     1141 tacccgttaa gatactgcag acacccttaa tcaaccttcc cctaataata aacaactctg
     1201 cagtaagcaa gtggtctggc tattatgcga tagcacagtc tttgatcttt ttaaggtaaa
     1261 catacctgtt ctttgtatta tcccatggct tttttctaat gaccataatg ttacactgca
     1321 aacgcgattg catgatcgag tgagcaggga tattcgtgcc cttccggatt gtaattgtta
     1381 caaactctat cataccacct aacattcatg agtggttctt gagtgagttg cgcgtgtctg
     1441 ctttacgtta acaacctcta tctgtgtgga taggatgtgt gccattttat atgggtagcc
     1501 tattctttca ttcctgttga caggcaaaaa aggtgatggc aaaatatttg atattgtatg
     1561 accttggtac caacttgatg acgatgtggt gtacctaaga aaaaaagtgt aagaaaatga
     1621 tttcacgaag atacatccta aaatggtttt ttggactccc cttactttct ttgttcccct
     1681 tccagatatc ggatgtaaga gcacaggcga ctcagtctgg gaacggtaag attgctgcgt
     1741 ctattggtga gttttttgct ggcgaagaac tacagtttga aatttccttc atgtttttaa
     1801 aaaaagttgc tgtagcaaga atgaccttta agcgggcaga aaaaaaaggt agatatattt
     1861 ctgttcttga aggagagaca gttggtattg ttggctggtt gtcaaggtat cgggcggata
     1921 cctattgtac agtgatggaa gaaactgaag gtgggatgcg tctcaggtcc gtgtcttttg
     1981 atgagaatgt aagaattggg aagaaaacaa gaacattttc ccacgaattt gattatgaga
     2041 aaaaaaagtg gactaaacgt tcaaccagga gaggcgcgag attacggacg gttgaggctg
     2101 ctattccgga aggcaaggtg tatgatgatt ttttgtgtgc ctcttacaat ttccgatatg
     2161 gcgtgtatgg tccccttgag aggggacgaa catatatcat tcctacattt ccccggaaaa
     2221 gatcaagcaa gtacgaaatt aagatagctg caaaagaaaa agaggatcat cagcgggttg
     2281 ccgacaaagt tggagatgaa gctgctttgc ttctacaact caaacttgat ccagaaatca
     2341 ccgattcaaa aacaggcatt atcgatggat ggctctcaaa ggataattgt cctttagaag
     2401 gaattataag agacgctatt ctttttggag atgtgcacgg gaagctcgtt agcagaataa
     2461 aacgataaaa gccaaacaat atcgatattt ccacatgtca gtggtttaca ggaccggacc
     2521 atttgtcttg tacattcacg taacaggatg aatgtcgccg gcacgctatt tttttgtcat
     2581 ttatttttat atttcaacat ggtttgccgc ggagggaaaa ggtgagataa atcattgata
     2641 acgcagagtt tacgctgtta aagtgattat caaagtcatg ctcaaaaaag gaaaacaaaa
     2701 gcaggacaaa aaagcagcca atttgcataa agatagttct tttaatatcc gtgatctggt
     2761 tatgagaact cctgcgagca catcacccga aagatctcca gaactgtaca aagatgaaaa
     2821 gcgagctaag cccctgaaac aagatacaat agctaagcct tctcctgcag cagcaataaa
     2881 aagtagtaat gataaattag cacaaattat caaggagtta agctccttcg atgtgagaac
     2941 aatacaacga cgatctgatc caaggatgaa ggcgctctgt ggacagatca atgacatact
     3001 tgccgaaatt tttggtcgta attctgatga atacgatgac tatgtaattt attctctcga
     3061 tacattaccc atcactattg gtggttcatg gcacccattg cccgaagttc aagaagggta
     3121 tcgcaaaggg attcaatctg ccatcacaaa acttacctcc ctccttgtta ttcagaaaag
     3181 taaattagaa attgtttctg gagaaaaacc agcacgcaat gttgttaatg agccatctgt
     3241 taatcaagtt attacaggaa aagagaaggt atctcttgta aatctgagag gaaaaatcag
     3301 aagtagggaa acagtattct tcagccaatg tgaaggaaca gagaacgatc atcgaaaatc
     3361 acattccaac tctgaagaat ctgatacacg agcacgcgca atcaagagat tttctcagaa
     3421 taaccattgt aaacctatcc aaactgagac tgaagtggca atgcatcaca caggtgatga
     3481 agaatcaatt caaaaaataa atcctgataa taataagcca gtattgttgg aaaatcttga
     3541 agaaaagctg aaaacacttg aaagtgaacc tgtttttaaa gataaagaaa atgagttcgt
     3601 tgagaccgag catgatccat tatttggaaa gccaaatcac gaggagagtc cattatcgac
     3661 actcgtcatg caactgaacg aaatcgttac aagtgatcct ggatacacgg aaacgcgttt
     3721 aagcaatgta acaggtagta atgaaaagaa tttgtcattc atgcaaaaag aaggtggcat
     3781 gtgtatcggg tttgatgaag aatcggcaac tccttctttt gaaagggtgg ctgaaatcta
     3841 cgccacagat tcagaaattt cagaaattga agaagttctg gttattgatg gagatgatat
     3901 cctatccaca gaagatagct taatacaaac ccttgagaat gaagatagag aacaagagat
     3961 tgccaagtca gccataaacg atgaatgcat tgacagaatc actgatggga aaaagaggat
     4021 atcccttgac gaactgcaac aaaagctcag ggagtttgaa gggaaagagt ttctacaaag
     4081 tgaacccctc aatgattttg aaaataagga aaatgaagaa gcctttctcg ttgacggcgc
     4141 agattgcttg cctgaggaaa tgatgaaaga acttgttcag gaggcaggtt ctcttgatgt
     4201 atgtgaaata cagcctcctg atcgagaggt gctgataatc gatggagatg ttgatccaga
     4261 tcaacacgct ttctctgtgg atgatgacga ggaaccacat gagatagaag cccacgaaag
     4321 tttgactcag gaaaaaaatg atgacgaggg aatgctggta attgagtgtg actcggggct
     4381 tcctgaggag ttattaaatg aacttggaca agaagacagc ttagtttgca aagaagatca
     4441 gctgcaaaga cctttgaaaa acgttgaggt gagagaaaat ttaacaaacc tttctgagca
     4501 gtacagtctc gacaggtcaa tgattattga tctgtgttcc agcacggcct cccttaatat
     4561 ttgttttctc aaagaggatg atggcacaaa aagggaaacc attctcgaag atttggagaa
     4621 gagactgaca cattttagtc ttttggggcc tggggaagag agtctacatt ggacagaggt
     4681 ttatgacgta gaaaaggtta attctgttgc agcgcaaggt cagtcagaca tgggaacaaa
     4741 tgaagaccat gcttcttctg gaagcccaat tgaaactgaa caaacctacg tcgatttgct
     4801 tcattcattc gacgtcaaga atgttttctc tgctggattt gataaagatt ttcctttaaa
     4861 ggaaattgcg atttctaatg aagcccctga gttttccgat gtggtgggat gtgatgagaa
     4921 tgccataatg gaatgcacgg ataggctatt gcctgtttgt ttggatgttt tacattgtgt
     4981 ggcagtagat aatatacttg tggaagatat tgcatttatt gaacaaaata agaagctgcc
     5041 tgaccagaat attatcatcg aaaaggtttg tgaagagtgt tcagattata tctcatttaa
     5101 taatctcgaa tcgttgaccg atcataccag tgagatgcat ataacaacgc ccgttgaatt
     5161 ttttgatgtc gagcgcatgt ttgaagaaaa taatgatgag aaacattttg atggtgattt
     5221 aactgacttt tgccagttgc agccagttga agattgggtt gattccaaca atggggatca
     5281 acaagagttc aaatttatag ccccagaagc aatgaaagaa gaaccagttc cttttttcat
     5341 ggatgaagaa ttgactgacc aggtagcaga gaaattgtgc gtacataacg aggaaacgtt
     5401 cgaagaactg cctcaactta tatttgatcc ttttgatggc attgacaatc atgagtttcc
     5461 gccgattaca gggaatgtgg acttgacagt agaaacagct gagcaccata ttgtagattc
     5521 gattttaatg aatgttaatg aagagttgtt ggttaagcct ggtacggatg aatctgcgag
     5581 tgatgacata tcgagaatta tagaatctca gattactcta cctgtagcca taaaagattt
     5641 aatgaatgat tcactgatga ttaaaagcga cattgagata ccagaaagcc ttaaattaga
     5701 agaacctttt tcgccgaggg gaagagaaga atattgcact tttgatatta atgatgcttt
     5761 tgttccatct attccggata caacaataga gattcataat aatgcacttt ttgaaccttt
     5821 aagacacgac ctcagcgaca atgagccttt accatgcagc atttttgaag agtgcattgt
     5881 atgtccagac gcttcagcaa atgtggaatg taaggttgat gaaaatctca gagagacgat
     5941 tcttcttgag gccttggaaa aaatgctgat tgacctcgag tcagacgtat ctgaacagag
     6001 ccacgttgat tttcagccaa tcaccactaa atgcactcct ttaccagaag aggaaaaaac
     6061 cactgttctt gagccccaga aagaatcccc acaggaaact gtattgatca agacccctga
     6121 agaacggagt aatccctttg cgatgacagg aatgggaagg gaaaaaacga atgcaaaaat
     6181 gatttgtgct gatgatctgc aggtacagat tggggaactc aggtatagaa ttgatgatct
     6241 aagaactttc gatatagact ccatagatca acgctttgat ccaagagtac gcgcgttagg
     6301 cgatacggtt aacaacacca tagcagatat attcggacgc aatactccgt catactggca
     6361 acatgctctc ccttcgctgg attcccttcc agtagttgtg ggcggtccca agctctcacc
     6421 agaagaactc aggggtgcat atcgaaaaag gatcaacgaa gctatagcca aagtaaatgt
     6481 taccattagc atgttagaaa caaaactggg taagcttcaa gataagggag tgaataagga
     6541 acattttcag ccaatcacca ctaaatgcac tcctttacca gaagaggaaa aaaccactgt
     6601 tcttgagccc cagaaagaat ccccacagga aactgtattg atcaagaccc ctgaagaacg
     6661 gagtaatccc tttgcgatga caggaatggg aagggaaaaa acgaatgcaa aaatgatttg
     6721 tgctgatgat ctgcaggtac agattgggga actcaggtat agaattgatg atctaagaac
     6781 tttcgatata gactccatag atcaacgctt tgatccaaga gtacgcgcgt taggcgatac
     6841 ggttaacaac accatagcag atatattcgg acgcaatact ccgtcatact ggcaacatgc
     6901 tctcccttcg ctggattccc ttccagtagt tgtgggcggt cccaagctct caccagaaga
     6961 actcaggggt gcatatcgaa aaaggatcaa cgaagctata gccaaagtaa atgttaccat
     7021 tagcatgtta gaaacaaaac tgggtaagct tcaagataaa accccgagcg gtcaggtatt
     7081 attcttctct caaaaaacca acaattataa agatgtccaa tcattgcact gattattgat
     7141 catcggataa acttgttttc atacttcttt tccaaaatgc tttagtaagc agataaaaga
     7201 attctgtggt aatcatcaat tcaa
//
LOCUS       DAOOMC010000135         7149 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdus sp. isolate AMR_MDS_2685
            NODE_12422_length_7149_cov_5.650973, whole genome shotgun sequence.
ACCESSION   DAOOMC010000135 DAOOMC010000000
VERSION     DAOOMC010000135.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864760
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdus sp. (wastewater metagenome)
  ORGANISM  Syntrophorhabdus sp.
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae; Syntrophorhabdus.
REFERENCE   1  (bases 1 to 7149)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7149)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,729
            Genes (coding)                    :: 2,708
            CDSs (with protein)               :: 2,708
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 7 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7149
                     /organism="Syntrophorhabdus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12422_length_7149_cov_5.650973"
                     /isolate="AMR_MDS_2685"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2699756"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            117..482
                     /locus_tag="PK125_11675"
     CDS             117..482
                     /locus_tag="PK125_11675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB38802.1"
                     /translation="MTSGRERIISNKIKNQLGDLNDYQESQEHIQERKSLILFWKIFA
                     ISFIAISFVYNRDLLSSLNDGDVGYLLVFISVLSAFSAFIFRHIIHMLRSKDEEIKSL
                     RNRVYELEQQKQKHQHQQK"
     gene            505..1725
                     /locus_tag="PK125_11680"
     CDS             505..1725
                     /locus_tag="PK125_11680"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HPB38803.1"
                     /translation="MKLKRNDKQRGVILCNRCRKLMTSDECPHCGNDRCFIGIYHQGK
                     YVTIRRDLSEHIFYFDTAYSYLMAINKTDRKVLVTPELDVETQLELWLHCKKKSVEMG
                     ELAPSYYRNLEGYVRNHFADYFANWHVLMIDKESIQMFKDTLKTQKIKTRKNILHALH
                     NFMTWLEENDKIDKIPAFPKIKGDDARVSQALTVEEQAEALSLLPEKHRHIFLFGTET
                     GLRSGELCAVKVGDINLRQGTMLVQRTLSAGTVRETTKGKHKNVIPLSEDALRIAAAN
                     MKGKNRNDFLFTQPIWDNGKSPTQERYTINRLYRLWLKYRTEKFADITVHELIRHSFC
                     TQLMNNTSINAMLAKDVMRHADVKMTDRYTHRNVEQSRQYINGRKDNSHLKLVHSASL
                     EVSGTPLKKEEKRK"
     gene            complement(1764..1838)
                     /locus_tag="PK125_11685"
     tRNA            complement(1764..1838)
                     /locus_tag="PK125_11685"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1804..1806),aa:His,seq:gtg)
     gene            complement(2019..2687)
                     /locus_tag="PK125_11690"
     CDS             complement(2019..2687)
                     /locus_tag="PK125_11690"
                     /inference="COORDINATES: protein motif:HMM:NF024595.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4013 domain-containing protein"
                     /protein_id="HPB38804.1"
                     /translation="MDIVAFIRFTFNSRYIVRWIYSGLIIYIPVVNFFSLGYLAKSAR
                     ILLIGSIGLPTWEDKMEIWTNGIKLLFVFILYEAVPFFLFSTGFFLTTLGSIPAFFGN
                     IVIKFSYLALLIFSFFIPFALSVFSERGEIRDALEFESIFTGIKEVLLPYLGGYLCML
                     FALYVCKIMIKIPYLVGFILSSLCTYYVFLLATYYFIHLFKRTSLVTAKITGGHTTEK
                     AQSS"
     gene            complement(2689..3474)
                     /locus_tag="PK125_11695"
     CDS             complement(2689..3474)
                     /locus_tag="PK125_11695"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HPB38805.1"
                     /translation="MKFKDKVVFISGGVKGLGKAMAKAFLAEGASVAVNGREYEVVSK
                     FAEEFQGKSVLAFNTDITHYEEMDNVASKIIESWGKVDILINNAGIVNPLAPSEKIKK
                     DDFDRTIDINLKGTFYTTQIFGKRMIEQKSGRIINIASQVAFFGEKGFLPYAMSKGAL
                     IIMTRELAYEWSKHGVTICSIAPGFIKGGMNEGLIRKEVFVNFLSSRTPIGRMGTVDE
                     LVSLILFLASPEAQYINGEIITMDGGMTGYTQESLLDFISKGK"
     gene            complement(3471..4418)
                     /locus_tag="PK125_11700"
     CDS             complement(3471..4418)
                     /locus_tag="PK125_11700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB38806.1"
                     /translation="MISQKVTFVEARGNIVRDCPGTKNHICCGYKTIDLVEGCILSCS
                     YCILKSYINSPDIKITRDYDYIFSQLNVAMEKEKNHILRFGTGELSDSLALDQRFDLN
                     RPLIQFFGEKKKALLELKSKWAYIEHIRDFLNPYIIISFSLAPQSIINKEEKKTSPLY
                     KRLKAARRAQDAGCFVGLHFDPIIIYPGFEKDYEYLIHDVGRILDLERVIWVSLGLLR
                     FPPRLFEEFLEKRRKNLLFGEFIRGEDGKYRYIKSERIKIYTMLYNQLKSKFDSLFVY
                     FCMERSDVWKAVTGINLDNNEGLIKLFDKRIHDLYGGAL"
     gene            complement(4390..5295)
                     /locus_tag="PK125_11705"
     CDS             complement(4390..5295)
                     /locus_tag="PK125_11705"
                     /inference="COORDINATES: protein motif:HMM:NF014273.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB N-terminal domain-containing protein"
                     /protein_id="HPB38807.1"
                     /translation="MNSLPIDRINTHDKRFCITYPLEDASLCASIQKIGIIQPVILLD
                     TSPFIAVTGFKRLEIARQLGLREVPFITVDITEQKALLFAIHDNIQRGLNLVEKAHAL
                     ERMLHLGFSPEELSDTLNILGVNSHEKIIKALVELASAEDSLKHFAVAHNLSLKNIQR
                     LLRFEVNERSSIIDLLSTIHVTESTIREILEIFSLIKLKNDALPLDIFPSASSQELLK
                     KLKAIAYPILSSLHEQLHDLRRTSALPPNIDIKVDPFFEKEYIDIGIRANNEEDIYQA
                     LEKLHRLTDERIIRRIFDLTKGNLR"
     gene            5470..6039
                     /locus_tag="PK125_11710"
     CDS             5470..6039
                     /locus_tag="PK125_11710"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013756.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HPB38808.1"
                     /translation="MKRSLGAKSLLYPTPIVVVGTYDNDGRANAMTVAWVGIVCSQPP
                     CIGISLRKATYTYGNIIEKEAFTISIPTEKHVKEVDYLGIASGRREDKFSTSGLTPIR
                     SDIVDAPYVKEFPFILECQLIQTIEIGLHTQFVGEIKNIKADESILNEKDLPDMGGRF
                     KPLLYAPELRKYFGMGPVLGNAFSMGKEM"
     gene            6121..6636
                     /gene="tpx"
                     /locus_tag="PK125_11715"
     CDS             6121..6636
                     /gene="tpx"
                     /locus_tag="PK125_11715"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF001808.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0016684 - oxidoreductase activity, acting
                     on peroxide as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HPB38809.1"
                     /translation="MERKGVITFMGNPLTLIGDEVKIGSKAPAFSVLEKDLSEVKLSD
                     FSGKVKILSVTPSLDTPVCDLQAKTFEENAEKLPESVVVLNISMDLPFAIARFSEDAK
                     IGRIRTLSDHRDASFGNAYGVLIKELRLLARSVFVIDKSDVVRYVEIVPEVTNQPDYT
                     RALEEAKKLAG"
     gene            complement(6821..7045)
                     /locus_tag="PK125_11720"
     CDS             complement(6821..7045)
                     /locus_tag="PK125_11720"
                     /inference="COORDINATES: protein motif:HMM:NF018058.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LapA family protein"
                     /protein_id="HPB38810.1"
                     /translation="MVNIIITILIILIVSIFSVQNSAPVAITFLVWQFQASLAIVIFL
                     CVLSGVIIGVALAFLYRIKRQRQARLKNSE"
BASE COUNT         2183 a         1395 c         1590 g         1981 t
ORIGIN      
        1 agagatggaa cgtcagaagg agctgactcc gagaggtggt gaacgatgga caccgactac
       61 tgtgagatta attatcaata gggtagagaa taaaattaaa agataaggag aataaaatga
      121 caagtggaag agagagaata atttcaaaca agattaagaa tcaactcggt gacttgaatg
      181 attatcagga gagtcaggag catatccaag aaaggaaaag cttgatactc ttctggaaga
      241 tattcgctat atcgttcata gcaatttctt tcgtgtacaa cagggattta ttaagcagct
      301 taaatgatgg tgatgtggga tacttattag tcttcataag tgttctaagt gcatttagtg
      361 cttttatctt cagacacatc atccacatgc tacgaagcaa agatgaagaa ataaaatcat
      421 tgaggaatcg tgtatacgaa ttagagcagc agaagcagaa gcatcagcat caacagaaat
      481 aaataacaag aaagagaggg agatatgaaa ctaaaaagga acgacaagca aagaggtgtt
      541 atcctctgta acagatgcag aaaactcatg acttcagatg aatgtcctca ctgtggaaat
      601 gacaggtgct tcatcggaat ctaccatcag ggcaagtatg tcacaatcag aagagacctg
      661 tcagagcata tcttctattt tgacactgct tatagttacc tcatggcaat taacaagact
      721 gataggaaag tacttgttac ccctgagctt gatgttgaga cccagttaga gctatggctg
      781 cattgtaaga agaagtctgt tgagatgggt gaacttgcac catcatacta cagaaacctt
      841 gagggatatg taaggaatca ctttgctgat tactttgcta actggcatgt cttgatgatt
      901 gacaaggaaa gcattcagat gttcaaggac acactcaaaa cccagaagat taaaacaaga
      961 aagaatatct tacatgctct tcataatttt atgacatggt tagaagagaa tgacaagatt
     1021 gataagatac ctgcatttcc taagataaaa ggtgatgatg ctcgtgtgtc acaggctctg
     1081 actgttgagg aacaagctga agcattgagc ttattacctg agaaacacag acatatattc
     1141 ctatttggta cagagacagg tctaaggtca ggtgaacttt gtgcagttaa ggtaggcgat
     1201 ataaatctta ggcaaggtac aatgcttgta cagcgtacct tatccgcagg tactgtgagg
     1261 gagacaacca agggcaagca taagaatgta atacctcttt cagaagatgc tctgaggatt
     1321 gcagcagcaa acatgaaggg taaaaataga aatgacttcc tgtttacaca acctatctgg
     1381 gataatggta aatcacctac acaggagaga tacaccatca acaggctata tagattgtgg
     1441 ctgaagtata gaacagagaa gtttgctgac attacagttc atgagcttat taggcactca
     1501 ttctgtacac agctcatgaa taacacttct atcaatgcca tgcttgcaaa ggatgtcatg
     1561 cgtcatgccg atgtcaagat gactgacagg tatacgcaca ggaatgttga gcagtcgaga
     1621 cagtacatca acggtaggaa agataattca catttgaagt tggtgcattc agcatcactt
     1681 gaggtatcag gaacaccctt aaaaaaggaa gagaaaagga agtgaaatgt cttgtattaa
     1741 atctaatgaa ttcaattact tattggggtg gctgacgggg ctcgaacccg caacaccctg
     1801 atccacagtc aggtactcta cctttgagct acagccacca tttgtaagct tatttagcac
     1861 aaatacagga taaaagtcaa tctcaacagc ctatagctta aagctgtaaa ctcacagcaa
     1921 atatcaaaga acccgatcgt catttaacga tactttcggg tatgggagga ataagatttt
     1981 ctttttgatg tttgctatca tctatgagct ataagctatt atgagctttg agccttctcc
     2041 gtcgtgtgtc ccccggtaat ttttgctgta acgaggcttg ttcttttaaa gaggtggata
     2101 aaataatagg ttgcaaggag gaacacgtaa tatgtgcata gggaggaaag aataaagccc
     2161 acaaggtagg gaattttaat cattatctta catacgtaca acgcaaagag catacaaaga
     2221 taacctccca gatatggcaa aagaacttcc ttaatcccgg tgaagatgct ctcgaactca
     2281 agtgcgtctc taatctctcc cctttcagaa aaaactgaca gggcaaaggg aataaaaaaa
     2341 gaaaagataa gaagggcaag ataagagaat ttgattacaa tattgccaaa aaaggcaggt
     2401 atgctcccaa gggttgtgag gaaaaatcca gttgaaaaaa gaaagaatgg caccgcctca
     2461 taaaggatga aaacgaagag gagcttaatc ccattcgtcc agatttccat cttgtcttcc
     2521 caggtgggaa gcccgatgct cccgatcaaa agaatacgcg cagatttagc cagataccca
     2581 agggagaaga agttgaccac cggaatatat atgataagtc cactatatat ccatcgcaca
     2641 atataccggc tattaaaggt aaaccggata aatgctacaa tatccatgct attttccttt
     2701 agaaatgaaa tcgaggagtg actcctgggt gtaaccagtc atgcctccat ccatagtgat
     2761 tatttctccg tttatgtatt gtgcttccgg ggaggcaaga aaaagaatga gtgagaccag
     2821 ttcatcaaca gtccccattc ttcctattgg tgtcctgctc gaaaggaagt tgacaaacac
     2881 ttctttccgt atgagtcctt cattcattcc accctttatg aatccggggg cgatactgca
     2941 aattgtcacc ccgtgcttcg accactcgta agcgagctca cgggtcataa taattaaggc
     3001 tcctttgctc attgcataag ggagaaaacc tttctcgcca aaaaaagcaa cctgggacgc
     3061 gatgttgatg atacgcccac ttttctgttc gatcatacgt tttccaaaaa tctgggtggt
     3121 atagaacgtg cctttcaggt ttatgtcgat ggttctgtca aaatcatctt tctttatttt
     3181 ttctgaagga gcaaggggat taacaatgcc tgcattgtta atgaggatgt caacttttcc
     3241 ccatgattcg ataatctttg acgctacatt gtccatttcc tcatagtgag tgatatcagt
     3301 gttaaatgcg agcacggatt ttccctgaaa ctcctctgca aatttactca caacctcata
     3361 ctctcttccg ttaaccgcca cagatgcacc ttcagcaaga aatgcctttg ccatagcctt
     3421 ccccaaccct tttacaccgc ccgaaataaa aacaacctta tctttgaatt tcatagtgca
     3481 cctccgtaaa gatcatgtat tcttttgtca aaaagcttta tgagaccttc attattgtcc
     3541 aggtttattc ctgtaacggc cttccacaca tcagacctct ccatgcagaa atacacaaag
     3601 agactgtcaa acttactttt taattggttg tacagcatgg tgtagatttt aatcctttca
     3661 gatttgatgt acctgtattt tccatcttct ccgcgaatga attcgccaaa aagcagattt
     3721 tttctccttt tttccaggaa ttcttcaaaa aggcgaggag ggaatcgcaa gaggccaaga
     3781 cttacccaga tgacccgttc gaggtccaga atccttccga catcatggat caggtattcg
     3841 tagtctttct caaaaccggg gtaaataata ataggatcaa aatgaaggcc aacaaagcat
     3901 cctgcatcct gagcccgtct tgcagctttg aggcgtttgt acagagggct tgtctttttt
     3961 tcctccttat taattatgct ctgaggagcc aaggaaaagg agataatgat ataggggtta
     4021 agaaaatccc tgatatgttc gatataagcc cattttgatt ttaattcgag gagggccttc
     4081 tttttttcgc caaagaactg tattaggggc ctgttcaggt caaaccgttg gtccagggcg
     4141 agactgtcag atagttctcc cgttccaaag cgcagtatat ggtttttttc cttttccata
     4201 gccacattca gttgcgagaa tatataatcg tagtctctgg ttatcttaat gtctggagag
     4261 ttgatgtatg atttcaatat gcagtaagag catgaaagga tacatccctc cacaagatcg
     4321 atggttttgt aaccgcagca aatgtggttt tttgtaccgg ggcaatccct aacaatattg
     4381 ccacgtgctt caacgaaggt taccttttgt gagatcaaag attctccgta ttattctttc
     4441 atctgttaat ctatgcagtt tttcgagggc ttgatagata tcttcctcgt tatttgccct
     4501 gatacctata tcaatatact ctttttcaaa aaaagggtct accttaatgt caatgttggg
     4561 cgggagcgct gatgtacgcc gtaaatcgtg caactgctca tgaagggaac tcaggatggg
     4621 ataagctatt gctttgagtt tttttagcaa ctcctgacta ctggcagatg gaaaaatatc
     4681 gagaggcaac gcgtcatttt tcagtttaat caggctaaaa atctcaagaa tttcccgtat
     4741 tgtgctctct gttacatgga ttgttgaaag aaggtcgatg atagaagaac gttcgttgac
     4801 ctcgaacctc agcaggcgct gaatgttttt cagagaaaga ttgtgggcta ctgcaaagtg
     4861 ttttagggaa tcctctgccg atgccagttc aacgagtgct tttattattt tttcatgaga
     4921 attcactccc aggatattca gggtgtcgga taattcctca ggggaaaaac caagatgaag
     4981 catccgctca agggcgtggg ctttttcaac aagattgaga ccccgttgaa tattgtcgtg
     5041 gatggcaaaa agaagggctt tttgttcggt aatgtcaacg gtaatgaagg gtacttctct
     5101 caaacccagt tgtcgggcta tttctaatct tttgaagcct gtcacagcaa tgaagggaga
     5161 tgtatccaga agtatgacag gctgaattat gcctatcttt tgaattgatg cgcaaaggga
     5221 tgcatcttca agaggatagg ttatgcagaa tcttttgtca tgtgtattga tgcggtctat
     5281 gggtaatgaa ttcatgtcaa tgtactgtag gcggtgaaag tatatatcaa tccaagggta
     5341 tatacaatgt gatttgaatt tctatactgt aagctattac ctgtcagcca accgattgcc
     5401 cttctttata caatacgcta taatccttgc catgtatttt aacaccctat gaaagtttag
     5461 gaggatttca tgaaaaggtc tttgggagca aagagtctcc tgtatccaac cccgatcgtt
     5521 gttgtcggga cttatgataa tgatggaagg gctaatgcca tgactgttgc atgggttggc
     5581 attgtctgtt cccagccgcc gtgcatcggg atttccttga gaaaggccac ctatacgtac
     5641 ggcaacataa ttgagaaaga ggcatttaca atcagcatcc ccacagaaaa gcatgtgaag
     5701 gaggtcgatt atcttgggat tgcttccgga aggagggaag acaagttttc tacctctggt
     5761 ctgacaccca tcaggagtga tatcgtagac gcaccctatg taaaagagtt cccttttata
     5821 ctggaatgcc agctcattca gacaatagag attggccttc acacccagtt tgtgggagag
     5881 ataaaaaata tcaaagcaga tgagtcaatc ctgaacgaga aagacctgcc cgatatgggt
     5941 ggcaggttca agcccctcct ctacgcccct gaactgcgga aatactttgg gatggggcct
     6001 gtgctcggca atgctttctc catgggaaaa gaaatgtgac acatttctgc ctgtattgac
     6061 aatatggtgt tcgatgacaa cattaaaaaa caacattaac gtaaaacatg tgaggtgctt
     6121 atggaaagaa aaggcgtcat aacgtttatg ggtaatccct taaccttgat tggggatgaa
     6181 gtcaagattg gcagtaaggc accagctttt tcagtgcttg aaaaagatct cagcgaggtc
     6241 aagctttcgg atttctcggg aaaagtgaag attctcagcg taacgccatc actggatacc
     6301 cctgtttgcg atctccaggc aaagacattt gaagaaaatg cggaaaaact tcctgaaagt
     6361 gtggttgtcc ttaacatcag tatggatcta ccgtttgcca tagcccggtt ttcagaagat
     6421 gcgaagattg gtcggattcg aactctgtct gatcacaggg atgcatcatt cggcaatgct
     6481 tacggagtac tcattaaaga gttgaggctc cttgcgcgct cagtctttgt gatcgataaa
     6541 tcagacgtgg tacgatatgt tgagattgtt ccggaagtta ccaatcagcc agattatacc
     6601 agggcgcttg aagaggcgaa gaaactggcg gggtgattgt agagattgca ttgaaggcat
     6661 ctgttgcttg agggcttttc caagcgctcc accacgctca gtttttttca aagaatcgcc
     6721 tgatatgatt tgataggcca tagggaggga tacttagtgg caagattgtc aggagtatca
     6781 atccctgatc gtttttggca gtggaggctc cataaatggg ttattcagaa tttttaagcc
     6841 gggcttggcg ttgccgtttt atcctgtaca ggaaggcgag ggcaactcca attatgacgc
     6901 cactcaaaac gcaaagaaag ataacaatgg cgagagatgc ctgaaattgc catacaagaa
     6961 aggtaatggc gactggggca gaattttgca cagaaaatat agaaacgatc aaaataatga
     7021 gaatagtgat tatgatgtta accatgggta aaatccttcc tcttttatgc tgtcagagca
     7081 ttgacaaggc tgtacgttag catgattata catcaaaaca agaaacaggt catcctgtaa
     7141 gattgttag
//
LOCUS       DAOOMC010000136         7093 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdus sp. isolate AMR_MDS_2685
            NODE_12544_length_7093_cov_5.280335, whole genome shotgun sequence.
ACCESSION   DAOOMC010000136 DAOOMC010000000
VERSION     DAOOMC010000136.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864760
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdus sp. (wastewater metagenome)
  ORGANISM  Syntrophorhabdus sp.
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae; Syntrophorhabdus.
REFERENCE   1  (bases 1 to 7093)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7093)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,729
            Genes (coding)                    :: 2,708
            CDSs (with protein)               :: 2,708
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 7 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7093
                     /organism="Syntrophorhabdus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12544_length_7093_cov_5.280335"
                     /isolate="AMR_MDS_2685"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2699756"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..238
                     /locus_tag="PK125_11725"
     CDS             <1..238
                     /locus_tag="PK125_11725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB38811.1"
                     /translation="IRMDFMFRMICSTYQSSFQSSLYFSSTCSAGKENLLFIDRSSIP
                     TYFQTLPVYFIVYKGPRIGFELFSKSVKNKPGMY"
     gene            complement(738..1370)
                     /locus_tag="PK125_11730"
     CDS             complement(738..1370)
                     /locus_tag="PK125_11730"
                     /inference="COORDINATES: protein motif:HMM:NF013933.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HPB38812.1"
                     /translation="MSLFSLFSIGFIIGLTGAMAPGPLLTITIGSSAKQGGIVGPLVV
                     LGHGILELALLALIVFGVANFLNNQMIFSGIAFLGGLVLVYMGYNIIRGLKNYNLSVS
                     DSGKKAGMHPVITGIIVSISNPYWFIWWITIGMGYVMFARNLGMAGILAFFIGHILSD
                     LVWYSFISYGVQFGGRYLNLKVIKIILFVCSLFLITFGISFIIKGYGFIR"
     gene            1460..2239
                     /locus_tag="PK125_11735"
     CDS             1460..2239
                     /locus_tag="PK125_11735"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: protein motif:HMM:NF003814.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0017168 - 5-oxoprolinase (ATP-hydrolyzing)
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpA"
                     /protein_id="HPB38813.1"
                     /translation="MRYSVDVNCDMGESFGAYTLGVDHEVIKYISSSNIACGFHASDP
                     DVMAKTVELCKKHHVMAGAHPGYPDLLGFGRRYMDMVEQELLHCIIYQVGALKGFLDY
                     FGLPLQHVKLHGALYNYLVNDERLSISIASAIQKAFGDVIFLTLGTNRTSKLKENYKK
                     QGIRLALEAFPDRNYTDDGELLSRRHEEAVLKDHEKIAQRATRMVKEKGIPSINGKWL
                     EMDIDTLCIHGDNAESIEAAKMIHNFVAKEGIEIKPLCEIV"
     gene            2245..2886
                     /gene="pxpB"
                     /locus_tag="PK125_11740"
     CDS             2245..2886
                     /gene="pxpB"
                     /locus_tag="PK125_11740"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HPB38814.1"
                     /translation="MDYSRYGEDGVRVLFGNTIDLETNREIRKFYLFLKSLHRQDIID
                     IIPSFKSCLIHFNNRLTSYDDFIAFLKKKTENISSVQIPEPKSHEIPVHYGGEYGPDM
                     EFVCSYSGLNEEEVIEIHSSIVYTVFAVGFIPGFPYMGTLDRRIYAPRLETPRLKVPE
                     GSVGLAQLQTGIYSFESPAGWRIIGKTNVQLFDATKKPYSLLQMGDLVRFVPI"
     gene            2883..3824
                     /locus_tag="PK125_11745"
     CDS             2883..3824
                     /locus_tag="PK125_11745"
                     /inference="COORDINATES: protein motif:HMM:TIGR00724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-dependent carboxyltransferase family
                     protein"
                     /protein_id="HPB38815.1"
                     /translation="MIEIINSAWFTLVVDGGRNGYADVGVPGSAALDQFAYNLLNFLV
                     GNENGAPVLEVMGNEFTVKVNCETTCAITGAQVTAYVDDKQITPWVSFAARTGSTIRV
                     KQVLEGFRYYVGFSGLMDVDRVIDSYSTNLECRFGGFRGRPLIKGDRIGLKEPKVVGE
                     RFVPEEVIPAMEQPHVLRVLEGPEFHYFTSEAINRLFEKKDHSWYIVSTKSNRTGIRL
                     EGKPLIFRKEMDKSITSEGILPGTIQVPGDGLPIFMLNERTIGGYARIGMVAKADQDR
                     LAHLKPKDKVFFQLIGIDEAERLWRQKIELFYRTLPQ"
     gene            3897..5195
                     /locus_tag="PK125_11750"
     CDS             3897..5195
                     /locus_tag="PK125_11750"
                     /inference="COORDINATES: protein motif:HMM:NF024850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HPB38816.1"
                     /translation="MSRKQILFVFVLFLSIVTMSSALFAAEKEVKIGVLYPLSGAAAT
                     IGRDLQRAAELTAEIINNKTPGVDIPMAQWGGIPNLGGAKIKLIFVDHRGEPDRGADL
                     AKRLILDDKVAGLMGAYHSSVTKTVSAVAERYGVPMINDSSTSPELTKRGFKWFWRTT
                     PHDDYFNRDLFELLKGLTEGKVKGIPAVPKDKLKNLAYACENTEWGSSVAKSLEGYAK
                     EYGFNMVKGILYNANSPDLTSEAKTLVAAKPDGMLFASYLSDSLLMIRTLKGMRAQTN
                     FFWGQNAGFEMADFAKTLGADINGVLTRTVFIAKLIKVKKVAGQINALYKKKYGDDLT
                     GATARSVVGVQAWAAVLNKAGSVDPKAIQKACNEINIPANELVMPWAGVKFDSTGQNV
                     LGRGLIGQYQKGADGKIDLEIVYPFDLATANFIYPFPGWK"
     gene            5310..6200
                     /locus_tag="PK125_11755"
     CDS             5310..6200
                     /locus_tag="PK125_11755"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HPB38817.1"
                     /translation="METLIDALLQGILMGLLFALVALGLNVIFGVMDIVNFAHGEFLM
                     LGMYTAFFTSLLMGVDPLFALPASALMGVLLGLASYYWFIKYLLRGPAIAQLFGTFGL
                     MLFLRYLAQFLFGIDYRILQKGILIGKSFSVGPFIFDATKIAAGALSLVAFAAVYSFL
                     NKTKTGKALQATALNKEAASYMGIKTDKMNAIAWAIGGGTVGVAGGLLINFYYVYPTV
                     GLLFVMIAFACVALGGFGSIKGVFFAGLLVGILEMMFPVFGQLVGSDFLGPHFKFTVV
                     YLAYFIIVVFRPQGLFGWKK"
     gene            6188..>7093
                     /locus_tag="PK125_11760"
     CDS             6188..>7093
                     /locus_tag="PK125_11760"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HPB38818.1"
                     /translation="MEKVKDALDFTDFSGVERFVLVVFSIFLLAFPWLGAGSFVKAVM
                     IQFLLFSLFGMGWNTIGGYGGQVELGKAQYVGIGAYTVSVSMVWWNIPFWISMPIGVL
                     IALGYSFMIGYPLFRLKGHYFAIATICTSLVLQDIFTNWKLVGAAKGIELPIHEHPDF
                     LYMQFKSDTYYYYFIMVLFYIALFYMNWFRKSKLGYQLRMIKDDEEAAESLGIDIRWC
                     KIKAYSIASAFVAVGGGFMAVYNLYIDPVSVMDLDLSIKIALMAMLGGAASLWGPIIG
                     AAFLVPLDRFLGSWLGGHKGLIGVDF"
BASE COUNT         1961 a         1394 c         1659 g         2079 t
ORIGIN      
        1 tatccggatg gacttcatgt tccggatgat ctgttccacc taccagagtt ccttccagag
       61 ttccttgtac tttagttcca cctgttcagc aggaaaggaa aatcttctct ttatagatag
      121 aagcagtatc ccgacctatt ttcagacact tccagtatat tttattgtct acaaaggacc
      181 tagaattggc tttgagcttt tctcgaagag tgtcaagaat aagcctggta tgtattaaaa
      241 tatactatac aaccagttga tcattcgcaa tggcttcttt ggccttcaac agggaaggat
      301 ttttaaaaga gttgccctcg gggagatcat ctggaaagca ctttcttcta cctttttcat
      361 ctagataact agggtaaagg aaacctaatt tggtttcaag gcagaccaaa atttctttgc
      421 tgatcatcca tacgatgggc aacagtatca gatgcagaag aactgctgaa gaaaggcttt
      481 attgtttgaa ttgttaaaat cattcaagag gcttgcctat caaaaaaaca acattaaagt
      541 gttcctttct gtgtctgaac catcacttat acgtataaag ccggtagtcc tatgccatga
      601 tgttcattat ctcacaacaa catgccatat caatcagagc gtatccatcc agaaagccat
      661 ctgttttctg tacttgggaa ggacaatttt attttactta caggcaagat tgatagttca
      721 aacaatatgg gaatgattta tcgaataaaa ccataacctt tgataataaa ggatattcca
      781 aaggtgataa gaaaaaggct gcaaacaaac agtatgatct ttatgacctt gaggttgagg
      841 tatcttcctc cgaactggac tccataagag ataaagctat accagacgag atcggatagg
      901 atgtggccga taaaaaaagc aaggatacca gccatgccga ggttcctggc aaacattaca
      961 tatcccatgc caatggttat ccaccagatg aaccagtatg gattggatat gctcacaatg
     1021 atgccggtga ttaccggatg cattcctgct ttcttaccac tatctgatac agaaaggttg
     1081 taatttttta agcctcttat aatgttgtat cccatgtaga caagcacaag accgcccaga
     1141 aatgcaatac ctgaaaatat catttgatta tttaaaaaat ttgctacgcc gaatacaata
     1201 agggcgagaa gagccaattc aagaatacca tgcccgagaa caacaagagg ccccacaata
     1261 ccgccttgtt ttgcagagct tcctatggtg attgtcaaaa gaggcccggg cgccatggcc
     1321 cctgtcaaac ctattataaa tccaatggaa aaaaggctaa acaagctcat gacatagtaa
     1381 accataatta gatatatttg ataaatagca aattttttga aaacttgagc tgacatgttt
     1441 tttctgttat attggttaca tgcgctatag tgtcgacgtc aattgtgaca tgggggagtc
     1501 atttggggct tacactctcg gcgttgatca tgaagtaatc aaatacattt catcttcaaa
     1561 tattgcgtgc ggattccatg cgtctgaccc ggatgtaatg gcaaagactg tcgagctctg
     1621 caagaagcac catgtaatgg ctggagccca tcctggttat cctgaccttc tcgggtttgg
     1681 acgaagatac atggacatgg tagagcagga gcttttacat tgtataattt atcaggtagg
     1741 tgcccttaag ggttttcttg actattttgg tttgcccctc cagcacgtta agcttcatgg
     1801 cgccttgtac aattatcttg tcaacgacga gaggttatct atttcgatag cgtcggcaat
     1861 tcaaaaggct tttggcgatg taatattttt gactcttgga acaaacagaa cgtcaaagct
     1921 gaaggagaac tacaagaaac aagggatacg acttgccctt gaagctttcc cggacaggaa
     1981 ttacacagat gacggggaac ttctatctcg taggcatgaa gaggctgtcc ttaaagatca
     2041 tgaaaagatt gcacagcggg caaccaggat ggtgaaagaa aagggtatcc cgagtattaa
     2101 tggaaagtgg ctagagatgg atattgatac cctgtgcatc catggagaca acgcagaaag
     2161 tattgaagca gcaaaaatga tccataactt tgttgccaaa gaaggtatag aaataaagcc
     2221 actttgtgag attgtgtaaa gcacatggat tattcaaggt atggagaaga tggtgtaaga
     2281 gtgctctttg gtaataccat cgaccttgag actaacagag aaatccggaa attttacctc
     2341 tttcttaaat cgctgcaccg tcaagacatt atagatatta ttccatcctt caaatcgtgt
     2401 cttatccatt tcaacaaccg attaacctca tatgatgact ttattgcctt cctcaaaaag
     2461 aagacagaaa atatttcatc tgtacagatt cctgaaccaa agtcccatga aatccccgtc
     2521 cactatggcg gtgaatacgg gcctgatatg gagtttgttt gctcctattc tgggctgaat
     2581 gaagaagaag tgatagagat acattcatcg atcgtatata cagtgttcgc tgttggtttt
     2641 ataccaggat ttccttatat gggaacatta gacagaagaa tatacgcacc aaggcttgaa
     2701 acgccacgcc tcaaggtacc ggaaggttct gtgggattgg cccagctaca aacaggtatt
     2761 tattcctttg agtctccggc aggatggcgg attattggaa aaacaaacgt tcagcttttt
     2821 gatgcgacaa agaagccata tagtctcctt caaatgggtg atttagtgag gtttgtccca
     2881 atatgataga aattataaat tcggcatggt tcacactagt tgttgacgga ggaagaaatg
     2941 gctatgccga tgtgggagta cctggttcgg ctgcactgga tcaatttgcc tacaaccttc
     3001 tcaactttct tgttggcaat gaaaatggtg caccggtcct tgaggttatg ggtaacgagt
     3061 ttaccgttaa ggtgaactgt gaaacaacat gtgcaataac aggcgcccag gtaactgcct
     3121 atgtcgacga caagcagata acgccatggg tgtcgtttgc agcacgcaca ggaagtacta
     3181 tcagagtgaa gcaggtgctg gaaggttttc gatactatgt tggtttttca ggcctcatgg
     3241 atgtagatag ggtgatagac agctattcca cgaatcttga atgcaggttt ggaggtttca
     3301 ggggaaggcc attgatcaag ggagacagga ttggtcttaa ggaacccaag gttgttgggg
     3361 aaagatttgt tcctgaagaa gtgattcccg caatggaaca gccccatgtt ttgcgggttc
     3421 ttgagggacc ggagtttcat tattttactt cagaggccat aaatagactc tttgaaaaaa
     3481 aggaccactc atggtatata gtctcaacaa aatcgaatag aacagggatt aggctcgaag
     3541 gaaaaccact tattttcaga aaggaaatgg ataaaagcat tacgtcagag ggtattttac
     3601 caggtaccat ccaggttcca ggtgatggtt taccgatttt tatgcttaat gaacgcacta
     3661 tcggaggata tgcgcgaatt gggatggttg caaaggctga ccaggaccgc ctggcacacc
     3721 tgaagccgaa ggataaagtg ttttttcagt tgattgggat tgatgaggcg gagaggttgt
     3781 ggagacagaa aatagagtta ttttaccgga cgttaccaca ataattctct gtattttgat
     3841 aaaataatat ctaattagta ttaccacaat aattttttga ggaggcaagg atagcgatga
     3901 gcaggaaaca gattcttttt gtttttgtat tattcttatc aatagtaacg atgagttcag
     3961 cgctttttgc tgcggagaaa gaagttaaga ttggagtgct ttaccctcta tcaggcgctg
     4021 ctgcaacaat tggtcgagac ctccagaggg ctgctgaact tacagccgag attattaata
     4081 ataagacgcc cggggtagac attccaatgg cacagtgggg cggcattccg aatcttggtg
     4141 gggcaaaaat aaaactgatt ttcgttgatc acaggggcga acctgataga ggtgcggacc
     4201 ttgcaaagcg ccttatcctt gatgacaagg ttgctgggtt gatgggtgcc taccacagtt
     4261 cagttacgaa gacggtcagc gctgtagcag aaagatatgg tgttcctatg attaatgact
     4321 cttccacatc ccctgaacta acaaagaggg gtttcaagtg gttttggcgc acaacccctc
     4381 atgatgacta tttcaacaga gacctctttg agcttctcaa agggctcaca gaaggaaagg
     4441 ttaaaggcat tccggctgtt cccaaagata agttgaaaaa tcttgcctat gcctgtgaaa
     4501 acaccgagtg gggttcaagc gtagcaaagt ctctcgaagg ctacgcaaag gaatacggat
     4561 ttaatatggt caaggggata ctttataatg caaactctcc tgaccttacc tccgaggcta
     4621 aaacgctggt tgccgctaag ccggatggta tgctcttcgc ctcttatctc tctgactccc
     4681 ttcttatgat aagaaccctc aagggaatga gagctcagac aaactttttc tggggtcaga
     4741 acgcgggatt tgaaatggct gattttgcta aaacccttgg tgctgatatc aatggtgtcc
     4801 ttacaaggac agtgtttata gctaaactga taaaggtaaa aaaagtcgca ggtcaaataa
     4861 atgccctgta caagaaaaaa tatggagatg atctgacagg cgccacagcg aggtcggttg
     4921 taggtgtaca ggcatgggct gctgttttga ataaagctgg cagtgttgat ccaaaggcta
     4981 tccagaaagc ctgtaacgag ataaatatac ctgcaaatga gctcgttatg ccttgggcag
     5041 gggttaaatt tgattcaaca gggcagaatg tcctgggcag gggtcttatc ggacaatacc
     5101 aaaagggtgc agacggcaag attgaccttg aaattgttta cccctttgac ctagcaacgg
     5161 caaatttcat ctatcctttt ccaggatgga agtaataaag tcaattcgtt atgggtttca
     5221 gttttaaagt ctcaggttgt attaagagat gttaaacgtg aaacccataa taaacaataa
     5281 caatgattca ttgttgagat gatgaatgta tggaaacact gattgatgct ctattgcagg
     5341 gtatattgat ggggcttctt tttgctcttg tggccttagg acttaatgtt atttttgggg
     5401 taatggatat cgtcaatttt gcccatggag aattccttat gctcggtatg tacactgctt
     5461 tttttacgag ccttctcatg ggtgtggatc cactttttgc acttccggca tctgccctca
     5521 tgggggttct tctcggcctt gcgtcatact actggttcat caagtatctg ctcagaggac
     5581 ctgccattgc ccagttgttc ggtacctttg gcttgatgct gtttctccga tatctggccc
     5641 agtttctctt tgggattgat tatcggatcc ttcagaaggg aattcttatc ggaaagagct
     5701 tttcagttgg ccctttcatt tttgacgcta ctaagattgc tgctggcgct ctcagccttg
     5761 ttgcattcgc tgccgtttac tcttttttaa ataaaacaaa aactgggaag gcacttcaag
     5821 caacggccct gaataaagag gcagcttcct atatgggaat caagaccgac aagatgaatg
     5881 ccattgcttg ggcgataggg ggcggaacgg ttggtgtggc aggaggactc ctgattaact
     5941 tctactatgt gtacccaacg gtgggtctgc tctttgttat gattgctttt gcatgcgttg
     6001 ctctgggagg ctttgggagc ataaaagggg tattctttgc tggccttctt gtcggtattc
     6061 ttgaaatgat gtttccagtc tttggccaac tcgttggttc tgatttcctg ggtccgcact
     6121 tcaagtttac cgttgtatat cttgcttatt ttatcattgt cgttttcagg ccccaagggt
     6181 tattcggatg gaaaaagtaa aggatgcact ggattttact gatttttcag gggttgagcg
     6241 gtttgtcctc gtggtgtttt ccatatttct cctggccttt ccctggctcg gtgcaggctc
     6301 ttttgtaaag gccgtcatga ttcaatttct tcttttttcc ctttttggaa tgggatggaa
     6361 cacaattggt ggttatggcg gacaagttga gcttggaaaa gcacaatatg taggcatagg
     6421 ggcttacacc gtttcagttt caatggtctg gtggaatatt cccttctgga tatccatgcc
     6481 cattggtgtt ctgattgccc ttggatattc tttcatgatt ggttaccccc ttttcaggct
     6541 aaaaggccat tactttgcta ttgcgaccat ctgcacctct cttgtgctcc aggatatttt
     6601 tacaaactgg aagctcgttg gtgctgcaaa gggtatagaa ctgcctatac acgagcaccc
     6661 tgattttctt tatatgcagt tcaaatcaga cacctactat tactatttca tcatggttct
     6721 cttctatatc gctcttttct atatgaactg gtttcgaaag tcgaagcttg gctatcaact
     6781 gcgcatgata aaggatgatg aagaagcagc tgagagcctg ggcatagata ttcgctggtg
     6841 caaaattaaa gcctactcca ttgcttctgc atttgtcgct gttggaggag gattcatggc
     6901 ggtttacaat ttgtacattg atcctgtatc agtcatggat cttgacctct caataaagat
     6961 cgccctcatg gccatgttgg gtggggctgc gtctctgtgg ggacctatta taggggcggc
     7021 gtttcttgtt ccgctcgaca gatttctggg gagttggctc ggtggtcata agggcctgat
     7081 tggcgtcgat ttt
//
LOCUS       DAOOMC010000137         6861 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdus sp. isolate AMR_MDS_2685
            NODE_13121_length_6861_cov_6.505289, whole genome shotgun sequence.
ACCESSION   DAOOMC010000137 DAOOMC010000000
VERSION     DAOOMC010000137.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864760
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdus sp. (wastewater metagenome)
  ORGANISM  Syntrophorhabdus sp.
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae; Syntrophorhabdus.
REFERENCE   1  (bases 1 to 6861)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6861)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,729
            Genes (coding)                    :: 2,708
            CDSs (with protein)               :: 2,708
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 7 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6861
                     /organism="Syntrophorhabdus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13121_length_6861_cov_6.505289"
                     /isolate="AMR_MDS_2685"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2699756"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(204..674)
                     /locus_tag="PK125_11765"
     CDS             complement(204..674)
                     /locus_tag="PK125_11765"
                     /inference="COORDINATES: protein motif:HMM:NF024304.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HPB38819.1"
                     /translation="MRRIKKEIRDNKRIGDLLDTCHVGRLGTIGNDGYPRIKPLNFVY
                     LNGIIYFHSAREGEKIDDISRDNRVVFEVDEPAGYVKSSISPCSAKYLYRSVIVRGKA
                     VIVENDREKLLALQSLMEKYQPEGGYGEFQPEKLEIIAVVRIDIEEITGKEDAG"
     gene            complement(714..1988)
                     /locus_tag="PK125_11770"
     CDS             complement(714..1988)
                     /locus_tag="PK125_11770"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPB38820.1"
                     /translation="MNTSQSFVRYRWLIFWILAAQYLVVYFHRVSPAVLAPELVIAFK
                     MSGTNLGLLASAYFYPYGIMQAPVGLLSDSWGSRKTITLFALLAAIGTLLFGLSPWFS
                     FAVGSRVLVGLGVSAIFIPTMRLFSVWFKPAEYARISGAFMAIGGLGWFTATIPLAFL
                     TEIFGWRISVIIPGLATIILSILTWFIVADNPAQKRFSSETKEREVPEERHNNLLVNL
                     SVILKEKYFWPIAIWFIFRGGALFGFFGLWAGPYLIDIYGFSKQIAGNILSMVAFAMI
                     FVSPVMGHLSDKTFMSRKKILVSTSLLNCVCWLIFVLYQEHLTLIVIYINFFLMGITV
                     SSVGTIAIVATRELFPLRIAGTAMGMMNVFPFIGGVLFQPLLGYILDRAGKIHGTYTP
                     SAYRLIIWILFITSVLALISIMFSKETYKKTR"
     gene            2057..3799
                     /locus_tag="PK125_11775"
     CDS             2057..3799
                     /locus_tag="PK125_11775"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase C-terminal domain-containing
                     protein"
                     /protein_id="HPB38821.1"
                     /translation="MDKETMRQLLDVAQGSAPADTVIRNGKLINVFTENIEDNIAVVI
                     KNGRIVSVEQDNGKASFLSNTEIDAEGSYLCPGFIDAHTHIDNMFPFHEFAPFSLRGG
                     TTTVITECAMTAGACGIKGIESFIESTKGYPVRCFFVAPPMTPPFPRIESAASFTLRE
                     FSTLLKRDDFLGIGEAYWTRAVEGDNRILAQAALAQSLNKRLDGHASGARGKRLTEYA
                     LTGITSCHESVSLEEAMEKLRLGIYVMIREGFVRKELPELSKLKDMKVDKRRIMVVSD
                     VFDAAMLWNEGYMDSLVKKAIKYGFTPMEAIKMVTINPADYHHLRYLGAIAPLRFADI
                     LFLRSIEDIIIEKVMFDGEVVVTGKTFLKNIKPFIFPEEMTRTVTINKLSENDFRVEA
                     RAGVKQIRVLHIANPTITRELVMDARVKNGYLEKDLENDVVPIAVINRRQGKGMGKGF
                     IKGTGIKSGAVATTLIWDTCNILTIGSSEKEMRDAVNRLIDIQGGIVIIRNGKVIYEF
                     PMPVYGLIPLMTMKESMNKTLEFDEKMISIGTNLEKPFLTIQTISFTGLPFLRITDKG
                     LVDIKNKQLMPLFV"
     gene            complement(3834..4418)
                     /gene="plsY"
                     /locus_tag="PK125_11780"
     CDS             complement(3834..4418)
                     /gene="plsY"
                     /locus_tag="PK125_11780"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HPB38822.1"
                     /translation="MHSYFLIVFAYLLGSVPVGLLLSKIKGADPRAVGSGNIGATNVM
                     RAAGKITGALTLIGDVLKGLIPVMIAFMLGETKIIVTAAGLAAFLGHLFPVFLRFKGG
                     KGVATALGVYLGLDPFAVLIAIIIFVLTISKWGFVSLGSLVGVAAMPLLLYFLSAPVQ
                     YIYLVLIIGALIFIKHKDNIRRLVAGTENKIGKS"
     gene            4538..4729
                     /gene="rpmB"
                     /locus_tag="PK125_11785"
     CDS             4538..4729
                     /gene="rpmB"
                     /locus_tag="PK125_11785"
                     /inference="COORDINATES: protein motif:HMM:NF013026.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HPB38823.1"
                     /translation="MARVCEMCGKGKQIGYGVSHANNKTKREWLPNLQTVKIVKNGTV
                     KRTRLCSKCIKKGDFKKAV"
     gene            complement(4795..>6861)
                     /locus_tag="PK125_11790"
     CDS             complement(4795..>6861)
                     /locus_tag="PK125_11790"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HPB38824.1"
                     /translation="QKAYIFSAQAHKGQVRLSGEPYLIHPLEVAYTLTKMNLDVPSII
                     SGLLHDTVEDSYVSKEEIEDYFGKEIADLVDGVTKISKIELKPSEDSKVENFRKMILA
                     MSKDIRVILIKLADRYHNMLTLNFLTSDKQIEIARETLEIYAPLAHRLGIEWLKGELE
                     DIAFKYLNPYEYRKITEKLAKTKKERESYINEVKDLIKEKFEQFHLKMEVSGRAKRIF
                     SIYKKMVQEELNIDDIYDLIAFRVIVDSVKSCYLALGIIHASFKPIPGKFTDYIALPK
                     ANMYKSLHTKVIGPHGEKIEIQIRTHEMHRLAEEGIAAHWKYKEGKVFDPKEDRVFGW
                     LRRIIESQQELKDNKEFMEVFKIDLFPDDIYVFTPRGDIKELPKGATPVDFAYAIHSD
                     LGHKCIGAKVNGKLVPLKHILKSGDAVEIQTSPTHKPSKDWLSFVVTSKAKTKIRQWI
                     KSEQRERSIELGKALIEKELSKHDLSFSKMLKTGDLLTIAKEFSFETADDLFAGVGYG
                     LYTPLHVLGKYIPEAEKPTKLQKIISTIRKGKDTSIHIEGVDGVVVRFAKCCNPIPGD
                     KILGFITRGRGLTVHTIDCPNILAYDDQRKVPVSWDLNKQYAYPVRIKVLGDDKKGLL
                     SEISTVMSTHKANILSAQAMTYPDKSAAGIYEIEIGHMSQLQKIIKSLQKIKGIRSVE
                     RVKGNA"
BASE COUNT         2028 a         1462 c         1488 g         1883 t
ORIGIN      
        1 tcatcttacc tgcgctgcat ctggtggggt acctgtggca acagaaaatg tggaggtggg
       61 ttaaaaactg tatgagcagc atttactgtg aaaggaaacg agggaaagct ttgaggggtt
      121 tatggtaaag gtgtgtgggg tgtaattgca tttttttgtt agcagcgcct gagggctata
      181 caatcaactg accataaata aatctaaccc gcatcttctt tgccagtaat ttcttcgata
      241 tcgatacgga ccacagcaat gatctcaagt ttttcaggct gaaattctcc atagccgcct
      301 tctggttgat acttctccat gagtgattgc aaggccagaa gtttttctct gtcattctca
      361 actattactg cttttcctct tactattaca ctcctataca gatacttggc tgaacagggg
      421 ctaatactgc ttttcacata accagcaggc tcatcaacct caaacacgac cctgttatcc
      481 cgactgatat catcgatttt ttcaccctcc cttgctgaat gaaaataaat aatcccgttg
      541 aggtaaacaa aattaagtgg tttaatgcga gggtaaccgt cattcccgat cgtgccaagt
      601 ctcccgacat ggcatgtatc cagaagatca cctattcttt tgttatccct tatctctttt
      661 tttattctgc gcattcagat cctcccaaat ttctcataca actaacacga tggttatcga
      721 gtctttttat acgtttcttt tgagaacatt atgcttataa gggctaaaac actcgttata
      781 aaaagaatcc agattattag cctatacgcg gagggcgtat aggtaccatg gattttacct
      841 gctctgtcaa ggatataccc aagcagaggt tggaataaaa caccgcctat aaatgggaaa
      901 acattcatca tacccattgc agtaccggca atacggaggg gaaacagttc tctcgttgca
      961 acgattgcaa tagtgccaac agaacttacg gtgattccca tcaagaagaa attaatgtaa
     1021 atgacaatta aggtgaggtg ctcctgatac aaaacaaaaa tgagccagca gacgcagttg
     1081 agcaaggagg tgctaacaag aatcttcttc ctgctcataa aagttttatc agataagtgg
     1141 cccattacag ggctaacaaa aatcatggca aaagcaacca tggaaagaat attaccagca
     1201 atttgctttg aaaatccata gatatcgatt aaatagggac cagcccaaag accaaagaaa
     1261 ccaaaaaggg caccgcctcg gaagatgaac cagatggcaa tgggccaaaa atatttttct
     1321 ttgagaataa cagagaggtt gaccagcagg ttattgtggc gctcttcagg aacttctctt
     1381 tcttttgttt ctgaggaaaa ccttttttga gcagggttgt ctgcaacgat aaaccatgtg
     1441 agaatagaaa ggatgattgt ggcaaggccc ggtataatga ccgatatcct ccaaccgaaa
     1501 atctcagtta aaaaggcaag agggattgtt gcagtaaacc accccaagcc gccaatggcc
     1561 ataaaagcgc ctgaaattct agcgtattcc gcaggtttga accagactga aaaaagtcgc
     1621 atggtgggta taaagatcgc tgagacaccg agaccaacga gtaccctgga gccaacagca
     1681 aaactgaacc atggcgaaag gccaaacaag agagtaccaa tggcagcaag taaggcaaaa
     1741 agggttatag ttttcctgga cccccatgag tctgaaagta aaccgacagg agcctgcatg
     1801 attccatacg gatagaaata tgccgaggca agtaaaccga gattagtgcc ggacatcttg
     1861 aaagcgatga cgagttcagg cgcgaggaca gcaggagaaa ccctgtggaa ataaacaacg
     1921 agatattggg ccgctagaat ccagaagatt agccatcggt aacggacaaa ggattgagag
     1981 gtattcattc cttaaggatt ttacaaacgg agaggcaata tgtaaaataa aaaaacaaaa
     2041 cacagaggag ataagaatgg acaaagaaac gatgagacaa cttcttgatg ttgcgcaggg
     2101 atcagcacca gcagatacgg taatacgaaa cgggaaactc atcaatgttt ttaccgagaa
     2161 cattgaggat aacattgctg tcgttataaa gaatggccgt atcgtctccg ttgagcaaga
     2221 taacggcaag gcttcatttt tgagtaatac cgaaattgat gctgaggggt catacctctg
     2281 tcccggtttt atcgatgccc acacccatat cgacaacatg tttccattcc acgagtttgc
     2341 ccctttttcc ctcagaggag gtaccacaac agtcataacc gaatgtgcca tgactgccgg
     2401 cgcctgtggt ataaaaggta tcgaatcttt catagaaagc accaaagggt acccggtgag
     2461 atgttttttc gtcgccccac ccatgacacc gccatttcca agaatagaaa gcgcagcatc
     2521 attcacatta agagaattct ctaccctcct gaaaagagac gacttccttg gtattggcga
     2581 agcttactgg accagagctg tggagggtga caacagaatc ctcgcccagg ccgcccttgc
     2641 acaatctttg aacaagcgcc tcgatggcca cgcttcaggt gcacggggca agcgccttac
     2701 cgagtatgca ctcaccggaa taacatcatg tcatgaatcg gtcagcctgg aagaagccat
     2761 ggaaaagctg aggcttggga tatatgttat gatccgcgaa gggtttgttc gaaaagaact
     2821 ccctgaactt tcaaaattaa aggacatgaa ggttgataaa cggaggatca tggtagtttc
     2881 tgacgttttt gatgccgcca tgctctggaa tgaaggatac atggattccc ttgtcaaaaa
     2941 agctattaaa tacggattta cacccatgga ggcgatcaag atggttacca taaacccagc
     3001 agattaccat catttaagat atttaggcgc tatagcccct ttgaggtttg ccgacattct
     3061 ttttctccgg agtatagaag atattataat tgaaaaagtg atgttcgatg gagaggttgt
     3121 tgtaacagga aaaacttttt tgaagaacat aaagcctttt atttttcccg aggagatgac
     3181 ccgcaccgtt acgattaata agctttcaga gaatgatttc agggtagaag cccgcgcagg
     3241 agtcaagcag attcgagtcc tccatatagc caatccaact atcacaagag aactggtcat
     3301 ggatgccaga gtaaaaaacg ggtacctaga aaaagacctg gagaacgatg ttgttccaat
     3361 tgccgtgatt aaccgcaggc agggaaaagg catggggaaa ggttttatta aaggtacggg
     3421 cattaagagc ggggctgttg ctacaacact tatctgggat acgtgtaata ttttaacgat
     3481 cggaagcagc gagaaagaga tgcgtgatgc agtgaacagg cttatcgata ttcagggagg
     3541 cattgttatc atcaggaacg gtaaagtcat ttatgaattc cctatgccgg tttatggact
     3601 gattccgctt atgaccatga aagaatctat gaacaagacc ctggagtttg acgaaaaaat
     3661 gatatctata gggacaaacc ttgagaagcc ttttctaacc atacagacca tctcattcac
     3721 tggactcccc tttctgagga taaccgataa ggggctggta gatataaaga ataaacagct
     3781 tatgccattg tttgtgtaac ataggagaga attgacgtca gtgttcagct gctttatgat
     3841 ttccctatct tgttttccgt ccctgcaaca agccttcgga tattatcctt gtgtttgatg
     3901 aaaatcaggg caccgatgat cagaacgaga tagatatact gaacaggtgc gctgagaaaa
     3961 tacagcaaaa gtggcatggc tgctaccccg acaagcgagc caagggacac aaagccccat
     4021 ttgcttatcg taagaacaaa aatgattatt gcaatcaaaa cagcgaaagg atcaagccca
     4081 agataaaccc ccagggcagt ggccactccc ttaccgccct taaaccttaa gaatacaggg
     4141 aaaagatgtc ccagaaaggc tgctaggccc gctgcggtca ctattatttt tgtctctccc
     4201 agcataaaag cgatcattac tggaataagt cccttaagaa catccccgat gagggtcagg
     4261 gctccggtga ttttgcccgc agccctcatt acattggtcg caccaatatt tccactacct
     4321 accgcacggg ggtccgcacc ctttattttc gaaagaagta gacctacagg cacagaacct
     4381 aaaagatagg caaacacaat gagaaagtaa gaatgcatga aacaagatag tagaaaaacc
     4441 tatttcatgt caatattttc atgtggtgaa tttgattgac atttgggctt gtatgtttta
     4501 aaattcagac tttcagtaga tggtacagga ggcatcaatg gcacgggtat gcgagatgtg
     4561 cggaaaagga aaacagattg gctacggtgt aagccatgca aataacaaga ccaaaagaga
     4621 atggcttccg aacttgcaaa ctgtaaagat cgtcaagaac ggaacagtga aaagaacacg
     4681 gctttgctca aaatgcatca agaaaggcga tttcaagaaa gctgtttaag gtttccgcct
     4741 cacttttttg ttggtttttc agcaaacgca aaacctttgg gtcacaacta gaatttatgc
     4801 gttccccttt acccgttcta ctgagcgaat cccctttatt ttctgaagtg atttgataat
     4861 cttctgtaac tgagacatgt gtcctatctc aatttcatag attccagcag cagatttgtc
     4921 aggataagtc atggcctgag cactcaaaat attggctttg tgagtcgaca tgactgtgct
     4981 aatctctgat aaaagacctt tcttgtcgtc cccgaggacc ttgatgcgca cgggatatgc
     5041 gtattgtttg ttgagatccc atgaaacagg cactttcctc tggtcgtcgt aggccaggat
     5101 atttgggcag tcaatggtat gaaccgtaag tccacgccct cgcgttataa agccgaggat
     5161 cttatccccc gggattggat tgcagcattt tgcaaatcta acaacaacac catcaactcc
     5221 ttctatgtgt atggaagtgt ctttgccttt cctgatagta ctgattattt tttgaagctt
     5281 agtaggtttt tcagcttccg gtatatactt acccaagaca tggagaggcg tgtataatcc
     5341 ataccctaca cctgcaaaaa gatcatctgc cgtttcaaag ctgaactctt tggcaatagt
     5401 gagaagatcc ccagtcttca acatcttcga aaaactcagg tcatgctttg agagctcttt
     5461 ttcgatgagg gccttaccaa gttcgatact gcgctctctc tgttcagatt taatccattg
     5521 tcgaatcttt gtttttgctt ttgatgttac aacaaaactg agccagtctt tacttggttt
     5581 gtgggttgga cttgtctgaa tctctacggc atccccgctt tttaagatat gcttcagagg
     5641 aactagcttg ccattaacct tggctccgat acatttatga ccaagatcag aatgaatggc
     5701 ataagcaaaa tcaacgggtg ttgcgccttt cgggagctct ttgatatctc cccttggagt
     5761 gaacacgtag atatcatcag ggaagaggtc aattttgaac acttccatga attctttatt
     5821 atcctttaat tcctgctgag actcgattat ccttctgagc cagccaaaga ccctgtcttc
     5881 tttgggatca aataccttgc cttctttgta tttccaatgt gcggcaatac cctcctcagc
     5941 taatctgtgc atttcgtggg ttcgaatctg aatttctatt ttttccccat gcggtcctat
     6001 tactttggtg tggagagatt tgtacatgtt agccttggga agggcaatgt aatcagtgaa
     6061 ttttccaggt atgggtttga atgaagcgtg gataatacca agggcaagat aacaactttt
     6121 gacgctatct acgattaccc taaatgcgat aagatcatag atgtcatcaa tattgagttc
     6181 ttcttggacc atttttttgt agatactaaa gattcttttt gccctgcctg atacctccat
     6241 ctttagatga aactgctcga atttctcttt tatcaggtct tttacttcat tgatgtaaga
     6301 ctcacgttct tttttcgttt ttgcaagttt ctcggttatt tttctgtatt catatgggtt
     6361 gagatattta aaggcaatat cctcgagttc ccctttgagc cactcaattc ccagcctgtg
     6421 agcaagaggg gcataaatct ccagtgtttc ccgagctatc tcaatctgtt tgtcggatgt
     6481 aaggaagttg agggttaaca tattatgata cctgtctgcg agcttaataa gaataacgcg
     6541 tatgtccttg ctcatagcaa gaatcatttt tctgaaattc tcaacctttg aatcttcaga
     6601 cggttttagc tcaatcttgc tgatctttgt gacgccatcc actaaatcag cgatctcttt
     6661 cccaaaatag tcttctattt cttccttact tacataagag tcttcgacag tgtcatgaag
     6721 aagacctgaa atgatacttg gtacatcgag gttcatcttt gtaagtgtgt atgcaacttc
     6781 caaaggatgg atcaggtaag gctccccaga caacctaacc tgtcccttgt gggcttgagc
     6841 agagaaaata tatgctttct g
//
LOCUS       DAOOMC010000138         6779 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdus sp. isolate AMR_MDS_2685
            NODE_13352_length_6779_cov_6.742713, whole genome shotgun sequence.
ACCESSION   DAOOMC010000138 DAOOMC010000000
VERSION     DAOOMC010000138.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864760
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdus sp. (wastewater metagenome)
  ORGANISM  Syntrophorhabdus sp.
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae; Syntrophorhabdus.
REFERENCE   1  (bases 1 to 6779)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6779)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,729
            Genes (coding)                    :: 2,708
            CDSs (with protein)               :: 2,708
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 7 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6779
                     /organism="Syntrophorhabdus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13352_length_6779_cov_6.742713"
                     /isolate="AMR_MDS_2685"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2699756"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..992)
                     /locus_tag="PK125_11795"
     CDS             complement(<1..992)
                     /locus_tag="PK125_11795"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HPB38825.1"
                     /translation="MADILIIDDDRDVSDMLVEMVTRMSHRPESSFTLKDARQKILAN
                     DYDVIFLDVRLPDGNGLDILPLIKDMSSVPEVIIITGHGDSDSAELAMKSGVWDFIQK
                     PCSLSSMRLVLTRAIQYREKKGLATKPPVLLKKDNIIGNSPSLRACFDLVAQAAQSDI
                     NVLITGETGTGKELFARAIHDNSQRADKSFIVVDCAAMPDNLVESTLFGHEKGAFTGA
                     DRSREGLIKLAHRGTLFLDEIGELPPLIQKSFLRVLQEHRFRPIGSSGEEQSDFRLIA
                     ATNRKLDRMVANGQFRTDLLFRIQALTIELPPLRERKEDIKELTIHYLRKFCEG"
     gene            complement(1005..5237)
                     /locus_tag="PK125_11800"
     CDS             complement(1005..5237)
                     /locus_tag="PK125_11800"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HPB38826.1"
                     /translation="MSTTKLRRFPSKFYLSSILIFLFFLDAFTLWFVARTDYKNTLDR
                     TRISLEKAAISLEERVRRTIISAEATLDGRAIRIQEKGIRSIILSKQEWERFRTAADG
                     LSDAGSLWLLDHEARLLSDSTSYPSPQIDFSGREYFIAHRDLGTRFYIGPVVKGRITG
                     KYSFTISRRLESKDGRFLGIIVAAIDTDDFANFMRNVELDEESTVIVFRTDGALILRQ
                     PMSDEFLGKDFKHLKLFSIPFDKEPSGSYEARATGGIKRFIAYRKIDRLPLVVAATTP
                     LDPIVKGWRERLKVYSLIALVFFFVLLGFSWLVRRTTTREEREKTEELSVINRSLQNE
                     INERKRAEGALLRSMERLDIISETANQLLFSTEPRKMVETLCRRVMEHLDCEVFLNFL
                     VDEEMKCLRLNAYAGIEEEIANSIHFLEYGETVCGCAARDACRIVAENISASSDIQTG
                     LIRSLGITAYFCHPLLVQEQVIGTLSFGTRSRSTFAEDELSLMKIVADQVATAMDRIR
                     LLRLEKERADELELQIARRTVQLKQQAELLNVAQDAIILTDIDGKIIFWSTGAENTYG
                     FSQEESIGSNIYDLLQTESQVPIRDIINTVKHEGRWEGELIHTCKEGRKATIHSKWIL
                     RQNEVDGILEIMEVNRDITSRKQVEEAVKEERERLYSVLETLPAYVVLLTRDYRIPFA
                     NRIFRELFGDPEDRQCFHHLFGRTGPCEGCETYKTLKTIEPQRWLWTGPDGHIYDIHD
                     FPFTDADGSTLILEMGIDITDRKRAEEEVRLANAYNRSLIEASLDPLVTISADGRIMD
                     VNEATEKVTGCARLQLIGTDFSDYFTSPERAKAGYEQVFKEGLVRDYELEIRHSSGHV
                     TPVLYNATLYRDETRKVLGVFAAARDISALRNAEQALRESEERYRIAIESASDGIALV
                     VHDLHVFVNRRFAEIFGYEGPSEIVGKPLSITVHPDDLNRVLEINKTRQEGKSAPARY
                     EFKGLKKDGTLRIIEVSAVRTTYLGESASLSYLRDITEYKNLEDQLRHSQKMEAIGTL
                     AGGIAHDFNNILAAIIGFAEMIEEDIPQGRPKLEHVQRVIRAASRGRELVQQILAFSR
                     KTEHARKPVSVASLARETVQLLRASIPTTIDILLDIAAVSDTILATPVEVQQVFMNLI
                     TNAALSMNEKGGMVHVSITDVNIEPDSPILNPDMGSGEYLRVVVSDTGSGMTPDVMER
                     IFDPFFTTREVGQGSGMGLAVVYGIVKSLHGKIEAESEPGVGSTFSIFFPKAEAGLSA
                     EETASEQAPGNSERVLFIDDEKFLVEWGQAQLERIGYEVTALDNSVVAFEIFLSDPSG
                     FDLVITDQTMPGMTGLDLAREIYKIRPDMPVILCTGHSDVVTTDIMKEAGIKEFLMKP
                     LTKKELLDAIQRVLHKEEYSRKDPEVHGTTGV"
     gene            complement(5485..6429)
                     /locus_tag="PK125_11805"
     CDS             complement(5485..6429)
                     /locus_tag="PK125_11805"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: protein motif:HMM:NF005143.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-32"
                     /protein_id="HPB38827.1"
                     /translation="MERHCVCGECTEHRKHKKCLPVKRDPLTAYLSELSKYPVLSLKE
                     EREVTQLVYENHDPRAVEKLTVSNLRLVVKIAMSYYNTYQNILDLIQEGNVGLLHAIK
                     KYNPHKGTKFSTYAQFWIRAYILQYIMSSWSLVKIGTTEGQRRLFYRLNKEKRRLEHL
                     GLNPAPATLARSLGVKEEDIIEMNIRLTYNDIYLDSPVNDDGVDTIMDLLTSDEDVEY
                     VVMEKESKRVLDEKIKALKINLNEKEICILDNRIMADKPETLEEIGARFCISRERVRQ
                     IEQRVLKKARTAFNGDMANLCLWQPHKGPDGAKALNRN"
     gene            complement(6542..>6779)
                     /locus_tag="PK125_11810"
     CDS             complement(6542..>6779)
                     /locus_tag="PK125_11810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB38828.1"
                     /translation="KIDIIPPEFLEQVVQWNSHEIFLSHYSKYRYEQESLKAWIQVCP
                     IITRNKRELKNRFCPAKQDKILFLNMLQITDQFV"
BASE COUNT         1704 a         1705 c         1436 g         1934 t
ORIGIN      
        1 tatccttcgc agaactttct aagatagtgt atcgtcagct ccttgatatc ttcctttctt
       61 tctcgcaagg ggggaagctc tattgtaaga gcctgaattc tgaacaggag atcggttctg
      121 aactgaccgt tggcaaccat cctgtcaagc tttctgttgg tggctgcaat gagcctgaaa
      181 tcgctttgtt cttccccgct ggagccgata gggcgaaacc tgtgttcctg caagaccctc
      241 aaaaaagact tttgaatcag agggggtaac tctccaatct catcaagaaa aagcgttccc
      301 ctgtgcgcca gcttgataag gccctcccgc gaacgatccg cgcctgtaaa agctcctttt
      361 tcatggccaa aaagcgtgct ttcaacgaga ttatctggca tggcagcaca atccaccacg
      421 atgaagcttt tgtctgccct ttggctgtta tcatgtatgg cacgagcaaa cagttctttt
      481 ccagttcctg tctcaccggt tataagtaca ttgatatcgc tctgtgctgc ctgggcaacg
      541 agatcaaagc acgccctcag tgaggggcta ttacctatta tgttgtcctt tttgaggaga
      601 accggtggtt ttgtggcaag tcccttcttc tcccggtact gaattgcccg tgtcaggacc
      661 agcctcatgg agctgaggga acagggtttt tggataaagt cccacacacc gcttttcatg
      721 gcaagctcag cactgtcaga atctccgtgt cctgtgataa tgatgacctc tggcacagaa
      781 ctcatgtcct tgataagggg aagaatatcc aggccattcc catccgggag tctaacatcc
      841 aaaaaaatca catcataatc gttagcgagg atcttttgcc gtgcatcttt taatgtaaat
      901 gatgattcag ggcgatggct catccttgtc accatttcaa caagcatatc agacacatct
      961 ctgtcgtcat cgataatcag tatatctgcc atgggccacc aatattatac ccctgtcgtc
     1021 ccgtgaactt caggatcctt tcggctgtat tcttccttat gcagcaccct ctgtatggca
     1081 tccaaaagtt cctttttggt aaggggcttc atgaggaatt ccttgatacc tgcttctttc
     1141 ataatatctg tggttacaac gtcgctgtga cctgtgcaca gtatgacagg catatccggc
     1201 cttattttgt aaatctccct ggcgaggtcg agcccggtca tcccaggcat tgtctgatcg
     1261 gtaatcacaa gatcgaatcc tgatgggtca gataggaaga tctcaaatgc cacaacactg
     1321 ttatccagag ctgtcacttc ataccctatt cgctccagct gcgcctgccc ccactcaaca
     1381 aggaatttct cgtcatctat aaacaagact cgctcgctgt ttccaggggc ctgttcagag
     1441 gcggtttcct ctgctgataa accagcttct gcctttggaa aaaatattga aaaggttgaa
     1501 ccaacacccg gctcgctttc ggcttcgatc ttcccgtgaa gactcttcac gatcccgtac
     1561 acaacggcaa ggcccattcc tgagccctgg cccacttccc tggtcgtaaa gaagggatcg
     1621 aatatccttt ccataacatc aggggtcata cctgagccag tatcgctcac aaccacccgc
     1681 aggtattccc ccgagcccat gtcagggttg agtatcgggg aatcgggctc aatgttaaca
     1741 tcagtaatgc tcacgtgaac catccctcct ttttcgttca tggaaagggc agcattcgtt
     1801 ataagattca tgaatacctg ctgcacctca accggtgtgg caaggatggt atcagagaca
     1861 gcggcaatat cgagcagaat atcaatggtg gtaggaattg aggctcgcaa gagctgtacc
     1921 gtctccctcg ccagtgaggc aacggacaca ggtttgcggg catgttctgt cttccgggaa
     1981 aacgcgagga tctgctgaac cagttcccgc cccctagaag ccgcacgaat caccctttga
     2041 acgtgttcaa gctttggtct gccctgagga atgtcctctt caatcatttc agcaaacccg
     2101 ataattgctg ccaggatatt attaaaatca tgggcaatcc cccctgcaag ggtgccgatt
     2161 gcttccatct tctgagagtg acgcagttga tcttcaagat ttttatattc cgtaatatcc
     2221 ctcaagtaag acagtgacgc cgattctccg agataggttg ttctcactgc tgaaacttca
     2281 atgattcgca gggtaccgtc tttttttagg cctttaaact cataccgggc aggagctgat
     2341 ttaccctcct gtctcgtttt gttgatttcc aacactctgt taaggtcatc agggtgaaca
     2401 gtgattgaca gaggttttcc aacaatctcg ctgggacctt catagccaaa tatctctgca
     2461 aaccgtctgt tgacgaatac gtgaaggtca tgtacaacca gagcaatgcc gtcggaggcg
     2521 ctttcaatag cgattcgata tctttcttcg ctttccctta gagcctgttc tgcgtttctc
     2581 agggcagaaa tatccctggc cgcagcaaaa acccctagta ccttccttgt ttcgtctcta
     2641 tacaacgtgg cattgtaaag tacaggggta acatgtcctg aactgtggcg aatctcgagt
     2701 tcgtagtccc tcaccagccc ttctttgaac acctgttcat atccggcctt ggctctttcc
     2761 gggcttgtga agtaatcaga aaaatcagta ccgatgagct ggagtcttgc acatccggtg
     2821 actttctctg tggcttcatt gacatccatt atcctcccat cagcgctaat ggttacaaga
     2881 ggatcgaggc tcgcctcaat aagactccga ttgtatgcgt ttgccagccg aacttcctct
     2941 tcagctcgct tgcgatcagt aatatcgata cccatctcca gaatgagcgt ggaaccgtca
     3001 gcatctgtaa agggaaaatc gtggatatcg taaatatgac cgtcagggcc ggtccacagc
     3061 catcgttgag gttcaatggt cttgagggtt ttgtaggttt cacagccttc acaggggcct
     3121 gtgcgcccga agagatgatg aaaacattgc ctgtcctcag gatccccaaa aagctcccga
     3181 aaaatgcgat tggcgaatgg tatccggtag tcccgtgtta acaaaaccac ataggcaggc
     3241 aacgtttcga ggacactgta aagccgttcc cgttcctctt tcaccgcttc ttcaacctgt
     3301 tttcgagagg taatatcccg gtttacttcc attatctcca gtatcccgtc gacctcattc
     3361 tgccgcaata tccacttgct gtgtatcgtt gccttcctcc cttccttaca ggtgtgaata
     3421 agttcacctt cccagcgtcc ttcgtgttta acggtattta tgatgtctct gatgggaacc
     3481 tgagattctg tctgtaacag gtcatatatg ttactaccaa tagattcttc ctgtgaaaat
     3541 ccgtaagtat tctcggcacc ggtgctccaa aaaatgatct ttccatcaat atcagtcaga
     3601 ataatcgcat cctgtgccac attaagcaat tctgcctgtt gtttgagttg aacagtgcgc
     3661 cttgctatct gcagctcaag ttcatcagcc cgttcttttt ctaggcggag gaggcgtatc
     3721 cggtccatgg cagttgctac ctggtctgcc acaatcttca taagggaaag ctcatcttca
     3781 gcaaaagtcg agcgggaccg agtgccaaat gagagggtgc caataacctg ctcctgtaca
     3841 agaagtggat gacagaaata tgcggtaatt cccaaagagc ggatcagccc ggtttgaata
     3901 tcggatgagg cggaaatatt ctctgccact atccggcatg catcccttgc tgcacacccg
     3961 catactgttt cgccatattc gagaaaatgt atgctgttgg ctatttcctc ttcaatgcct
     4021 gcatacgcat taagccgtaa acacttcatc tcctcatcca caagaaagtt cagaaaaacc
     4081 tcgcagtcaa ggtgctccat cactctgcgg cagagtgttt ctaccatttt ccgcggttct
     4141 gtactgaaaa ggagttgatt tgctgtttca gaaatgatat cgagccgttc catgcttcgc
     4201 aacagtgcgc cttcagcgcg tttccgttca ttaatttcgt tctggagtga tcgattgatt
     4261 acagaaagct cttctgtttt ttctctctct tctctcgtcg tggttctcct gacaagccag
     4321 gaaaaaccaa gcaacacgaa aaaaaagaca agggcaatca gcgagtaaac cttgagccgc
     4381 tccctccatc ctttcacaat aggatcgaga ggggttgtgg ccgcaaccac taatggcaac
     4441 ctgtcaatct tcctatacgc tataaacctt ttaataccgc ctgtagctcg ggcttcatac
     4501 gatcctgaag gctctttgtc aaaaggaatg ctgaagagct tgagatgttt gaaatccttt
     4561 ccgagaaatt catcactcat tggctggcgc aggataaggg ctccatcggt tcgaaaaaca
     4621 ataaccgtac tttcttcgtc aagttcaacg ttgcgcatga aattcgcaaa atcatccgta
     4681 tctatagcag caacaatgat gcccagaaaa cgcccatcct tgctttctag cctgcggcta
     4741 atagtgaagc tgtatttccc cgttattcta ccctttacaa ccggtccaat ataaaatctg
     4801 gtgcccagat ccctgtgggc aataaaatat tcccttccag agaagtctat ttgcggtgaa
     4861 ggataagacg tggaatccga caaaagacga gcttcgtgat ccaacagcca gagagatcct
     4921 gcatctgaaa ggccatccgc agctgttcgg aaacgctccc actcttgttt tgataagata
     4981 atagaacgaa tgcctttttc ctggattcgt atagcgcggc catccagagt cgcctcagca
     5041 gatataatgg ttcgtctcac tctttcttca agtgagatgg ctgctttttc aaggctaatt
     5101 ctcgttcgat caagggtatt tttgtaatcg gttcttgcta caaaccagag agtgaatgca
     5161 tctaaaaaaa acaaaaaaat aagaatactg ctgagataga atttagaagg gaatcttcgt
     5221 aattttgtgg tgctcatcga ggtgttccag ctaaccgaaa caaagccaat tttccacaaa
     5281 cacgcagttt aagaccagcg aggagtcaca tatttcagtc cgaaagcctg ttgtgtcagc
     5341 ttccataaaa aaaccgggtg tcttgttgct aagtatagcg gattcacccc taaaaaacaa
     5401 accaccatat tcttcagaaa tcaggttatt ttgcgagttc cttaaaatgc cattttgggg
     5461 tctaagcccc cccctcatct tgaatcaatt tctgttgagg gctttagctc catcaggtcc
     5521 tttatgtggc tgccatagac acaggtttgc catatctcca ttaaaggcag tcctggcttt
     5581 tttcaggacc ctctgctcga tctgtctgac acgctcccgg gatatacaga accttgcccc
     5641 tatctcttca agcgtttccg gcttatctgc cataatgcgg ttatcaagta tgcatatctc
     5701 tttctcgttc agattaatct tcaacgcctt aattttttca tccaggactc ttttcgattc
     5761 cttttccatt accacatact ccacgtcctc atcggatgtg agtaaatcca taatcgtatc
     5821 aactccatcg tcattcacag gagagtcaag atatatgtca ttgtatgtta atcgaatatt
     5881 catctctatt atgtcctcct cttttactcc cagggaacgg gcaagggtgg caggggcagg
     5941 gttcagccca agatgctcaa gtctccgttt ttccttgttc aatcgataga aaagcctcct
     6001 ctggccttcg gtcgttccaa tttttacaag gctccatgag ctcattatat actgcaggat
     6061 ataagccctt atccagaatt gcgcatatgt tgagaatttg gttcccttgt ggggattgta
     6121 tttctttatg gcatggagaa gaccaacatt accttcctga ataagatcga gaatattctg
     6181 atacgtgttg tagtagctca tagcgatttt aaccacaagc ctgaggttag aaactgtcag
     6241 tttctcaacc gctctggggt catggttctc gtaaacaagc tgtgtaacct cacgttcttc
     6301 tttcagggaa agcacaggat attttgatag ttccgaaaga tatgcagtca acgggtcgcg
     6361 cttgaccggc aggcattttt tatgcttcct gtgttccgta cattctccac aaacacaatg
     6421 cctttccatt ctccatccct cctgatcaaa acttgcatgg aatatttttc tctctatcaa
     6481 aaattgcgcc atacaataaa acggaatgta aaaatttgag attatcaatt atatcaatat
     6541 attagacgaa ttggtcagtg atctgtaaca tattgagaaa gaggattttg tcctgtttgg
     6601 caggacaaaa tctgtttttt aactctctct tatttctggt tatgatcggg caaacctgta
     6661 tccaggcctt cagggattcc tgttcatacc ggtatttcga gtaatgggac agaaaaatct
     6721 cgtgagagtt ccactgaaca acctgctcaa ggaattctgg tggaatgata tctatcttt
//
LOCUS       DAOOMC010000139         6746 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdus sp. isolate AMR_MDS_2685
            NODE_13459_length_6746_cov_7.116276, whole genome shotgun sequence.
ACCESSION   DAOOMC010000139 DAOOMC010000000
VERSION     DAOOMC010000139.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864760
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdus sp. (wastewater metagenome)
  ORGANISM  Syntrophorhabdus sp.
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae; Syntrophorhabdus.
REFERENCE   1  (bases 1 to 6746)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6746)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,729
            Genes (coding)                    :: 2,708
            CDSs (with protein)               :: 2,708
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 7 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6746
                     /organism="Syntrophorhabdus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13459_length_6746_cov_7.116276"
                     /isolate="AMR_MDS_2685"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2699756"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            301..1440
                     /locus_tag="PK125_11815"
     CDS             301..1440
                     /locus_tag="PK125_11815"
                     /inference="COORDINATES: protein motif:HMM:TIGR03573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl sugar amidotransferase"
                     /protein_id="HPB38829.1"
                     /translation="MNQATYRICFNCLMDTTDPYITFDERGLCDHCRNYYENIVPNWY
                     PGELGEKILAPTIEKIKNDGKGRDHDCLIGISGGVDSSYLTMLAKEKFGLRPLIFHVD
                     AGWNSQVSANNINRLIDGLGLDLYIEVVNWEEMKDLQLAFFKAQVPHLDTPQDHVFFA
                     SLYNFAAKNGFKYILTGANYSTECVREPLEWHYHASDLCQIKDIHHQFGTRPLRTFPL
                     CDILTYKLYYRYIKGVRVVRPLNYVPYIREEAIEILMQRFGWEQYGHKHYESRFTRFY
                     EGYWLPRKFGYDKRRAHFSSLILTGQMSRDEALERIAKPELDETTMAQDFEYVAKKLD
                     LTAKDLEGLFNGKNKTYRDYKNNAALISLGTRILQGLGIERRVIR"
     gene            1655..2611
                     /locus_tag="PK125_11820"
     CDS             1655..2611
                     /locus_tag="PK125_11820"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HPB38830.1"
                     /translation="MATILITGGLGTVGAGLFQELTTRKHHVVICDLYHDAREVGFSV
                     GTDIEVPLYARCDIGEFRQIERVFERMGPFEYVYNCAAEFGRWNGEDFYETLWHTNAV
                     GAKNIIRLQEHYQFKLIHFSSSEVYGDWPELMIETVTDAHEIKQLNDYAMTKWVNEMQ
                     VKNSATQYNTESVVVRLFNTYGPGEYYSPYRSVNVRFLYCALNNLPFTVFRGHKRTST
                     YLADTVRTLANIVDNFKAGETYNIGGGNLHTIEDLAEVVLKVTNKDRSLVRFNDSERL
                     TTKVKRVDITKAIRDLDHKNNYSLEDGMHLTADWLRKVYKIT"
     gene            2636..3739
                     /gene="wecB"
                     /locus_tag="PK125_11825"
     CDS             2636..3739
                     /gene="wecB"
                     /locus_tag="PK125_11825"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: protein motif:HMM:TIGR00236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HPB38831.1"
                     /translation="MKKILHVVGARPNFIKAAAVMSAMAEQRDYFHQYLVHTGQHYDG
                     FVSDIFFSELSLPEPDCNLNVRSGSQAVQTAHIMKRFESVVLKYRPDITLVYGDVNST
                     LAAALVCSKVQIPVAHVEAGLRSFDRKMPEEINRVLTDQISDILFVPSVDGKDNLLKE
                     GVERNKIKMVGNVMIDTLVHFLPKALTRWNAGTVYANGFHYEQDYALVTLHRPSNVDD
                     RASLKEIMSAISNVSGYLPVVFPVHPRTMKRLERIRYDKSKSKDLHLMRSLGYLDFIA
                     LQANAKLVITDSGGIQEETTFLGVPCLTIRDNTERPITVDIGTNTLVGRNLTRLEKEV
                     ELILTDQGKRGQIPPLWDGKASQRIVQALRNQA"
     gene            3749..4975
                     /locus_tag="PK125_11830"
     CDS             3749..4975
                     /locus_tag="PK125_11830"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HPB38832.1"
                     /translation="MKNSGRIIFVSEVYYPDEQGTAYYITGLAEGLAEHYQLTVFCGY
                     PAVTARGRKVLARETINGVRVERSHGTTFNKDKLLLRLINLGSLSLFIFFKLLISVRK
                     DDIVIVVNGPHPLPYLARLVCFFHNARCILRIDDVYPEVFIATGIIPEKSIIARCIRY
                     INTLLFRGMDRVVVLGRDMKALAEKATKNGANHIEIIHNWGDVDTVLPKDKAINPLLA
                     QLGLKDRFVISCAGNMGRVQAIETMFGAINRIKNNADIHFVFVGSGSKRPWMEREIRT
                     KKLLNVTLVDQRPRSDQINFLNACDLALASLIRGMAGAGVPSRMYNVMAAGKPLIAIA
                     EEESELSRVVREEQIGWVVEPDNPEKLANVILEAYMKKEVLHNMGKRARIAAEQKYSR
                     GKIIQDYRGLLQSLDC"
     gene            5193..6188
                     /locus_tag="PK125_11835"
     CDS             5193..6188
                     /locus_tag="PK125_11835"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HPB38833.1"
                     /translation="MHADPTPRWGGLALTVGMVPALFFLEIDRQVISFVIAILILIVI
                     GGIDDCKRLGWRTKLFAILLSITTFVFLGGVSIRSLGFYGLDFNVRLGWVSIPFTYFC
                     IVGVTNAINLIDGLNGLAGGISFMAFVFLGIGGMMSGEPVLGFICFSFAGALLGFLFY
                     NFPKARIFMGDSGSLLIGFSLASLSILLTQGNNHIHAVGPIITEVDLYKAALSEEFAL
                     QPMFPVLILFVPIFDTLRVITVRLLLRKNPFHPDRSHLHHLFIKSGFCPITTVVALWT
                     VTFLCGVVALLLIKQSSVPYLGIVLGLSLFVSWLADALGRKRKDQQKQLKEAKDT"
     gene            6185..6715
                     /locus_tag="PK125_11840"
     CDS             6185..6715
                     /locus_tag="PK125_11840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB38834.1"
                     /translation="MIAKKVLSGMSVLLLIMFSMPVHAMDSEVTRETLNGLPGFYVAF
                     EELQPNIKQYDVTASMTRDQLQKDIEMRLQRAKITVLSQAQWLNTRGRPVLYGNINTH
                     LEDANIAYNIQLEARQIVFTDSNNRVKTLAGTWGITMTGITKIDKLEVIRQNLLTLVD
                     KFVEAYWTANGKDSKK"
BASE COUNT         2002 a         1268 c         1556 g         1920 t
ORIGIN      
        1 ctggctcact tcgcgacgct atgatgcgta tagccagcct gtcgatggag gaattggaaa
       61 catttacttt aagaagtcat atgcttggac agagaattac gtcagaacat acagcatatt
      121 ccctgctatc agtattggat ttggcaagaa tatgatagga actccaaaga ggtgttttgg
      181 catcaataaa tcaacttttt gccgaaagga tagtcacttc atttgtagtt cacgctatag
      241 acaattgata catcaaatga atattttcta atttcttatc aaatcatgat caaggaaaat
      301 atgaaccaag ctacatatag aatatgtttc aactgtttaa tggatactac cgacccatac
      361 atcacttttg atgaaagagg gttgtgcgat cattgcagga actattacga aaatattgtg
      421 cccaattggt atccaggaga gcttggagag aaaattcttg ctcctactat cgaaaaaatc
      481 aaaaatgatg gtaaggggcg tgaccacgac tgcctaatag gtataagtgg tggagttgat
      541 agctcttatc tgacgatgct tgctaaagaa aaatttggcc tgcgtccatt gatttttcat
      601 gttgatgctg gatggaattc acaagtctct gcaaacaaca taaacagact gatagatggg
      661 cttggattgg atctatatat tgaagtagtg aattgggaag agatgaagga tttgcagctt
      721 gcctttttca aagcccaggt gccgcatctc gatacacccc aggaccatgt tttttttgcc
      781 agtctttata attttgctgc aaagaatggt tttaagtata ttttgacggg tgcaaattat
      841 tcaacggaat gtgttcgcga accgcttgag tggcattatc atgcatctga tttatgccag
      901 attaaggata ttcaccatca gtttggaaca agaccactta ggacgtttcc actctgtgat
      961 atattgacgt ataagcttta ttatcgatat ataaaaggtg tgcgagtagt aagaccgctt
     1021 aactatgttc catatatcag agaagaagcc atagagatat tgatgcaaag gtttggttgg
     1081 gaacaatatg gtcacaagca ctatgaatca cgttttacac gattctacga aggctattgg
     1141 ctaccgagga aatttggtta tgacaaacga agagcgcatt tttcaagtct catactcaca
     1201 ggtcagatga gcagagatga agcattagag cgcatagcca aaccggaatt agatgaaact
     1261 acaatggccc aggattttga atatgttgcc aaaaagcttg acctgacagc aaaggaccta
     1321 gaaggcctgt ttaatggaaa aaacaagaca tatcgcgatt ataaaaacaa cgcagcatta
     1381 atatcacttg gaacgagaat tctccaggga ttgggcattg agagaagagt gatacgataa
     1441 tagcattatt tactatggac ttggctgtca aaggcaatgt aaaagcattc gcaaaggtgt
     1501 ataaaaaaat taaatgctcc tgttgtaaag gggcgcagta tctaaaacac gtctcttgct
     1561 taagatgatc gttagatgat tgagaggcgt ctcttatgta aggttttttt gagttacatg
     1621 atacaaaaca ttcgttgaat aataggagag ctctatggct acgattctaa taactggtgg
     1681 gttaggaacg gttggtgctg gattgtttca agagctgacc accagaaaac accatgtggt
     1741 aatttgcgac ctctaccatg atgcaagaga ggttggattc agcgttggga cggacattga
     1801 agttccactt tacgcacgat gcgacattgg ggaatttagg caaattgagc gtgtatttga
     1861 acgcatgggg ccgttcgaat atgtgtacaa ttgcgctgct gaatttggca gatggaacgg
     1921 tgaggatttt tacgagactt tatggcatac caatgccgtg ggtgcaaaaa atattattcg
     1981 tcttcaggaa cattaccaat ttaaactgat tcatttttct tcatctgagg tgtatggaga
     2041 ctggccggag ctgatgatcg aaacagttac tgatgcccat gagattaaac aacttaatga
     2101 ctacgctatg acaaaatggg tcaacgaaat gcaagtcaag aattctgcca cccaatataa
     2161 cactgagagc gtcgtggttc gcctttttaa tacatatggt cctggtgaat attacagccc
     2221 gtatcgttct gtgaacgttc gatttcttta ttgtgcactt aacaatctcc catttacggt
     2281 ttttcgaggt cacaagcgaa cctcaacgta tctggctgac accgtgcgga cacttgccaa
     2341 tatagtggat aatttcaagg caggagaaac atacaatatc ggaggtggca accttcacac
     2401 aattgaagac ctcgctgagg tagtcctaaa ggttaccaac aaagatcgct ctttagtcag
     2461 atttaatgat tcagagagat taacaactaa ggtaaaacgg gtagatatta caaaagctat
     2521 acgcgattta gatcacaaaa acaactatag cttggaggac ggaatgcatt taactgcaga
     2581 ttggttgaga aaggtttata aaataactta acgcaatact cgtgtggaga aatctttgaa
     2641 gaaaattctt catgtggtgg gggctaggcc gaacttcatt aaagctgcag cggtaatgtc
     2701 agcaatggct gagcagcgcg actattttca tcagtattta gttcatactg ggcaacatta
     2761 tgatggtttt gtttcagata tctttttctc tgagctgtcg ctgccggagc ccgattgcaa
     2821 tttaaatgtc cgttcaggaa gtcaggctgt tcaaacagcg catatcatga agcgttttga
     2881 atctgtggtt ttgaaatatc gtcccgacat aacactggtg tatggcgatg taaactccac
     2941 attagctgcc gcactggtat gctcaaaggt tcagattccc gtggctcatg ttgaagctgg
     3001 cctgcgatca tttgatcgga aaatgccaga agagataaat cgagtgctta ctgatcagat
     3061 ctcagatatt ctatttgtac cgtccgtcga tggaaaagat aatctcctga aagagggtgt
     3121 cgaaagaaac aaaatcaaaa tggtaggtaa tgtgatgatc gatacgctgg tacattttct
     3181 tcctaaagcc cttacgagat ggaatgctgg aacagtttac gcgaatggat ttcactatga
     3241 gcaagattac gcacttgtga cacttcaccg tccttccaac gtagatgatc gagcaagtct
     3301 aaaagaaata atgagtgcaa tttcaaatgt cagcggttat cttcctgttg tttttccggt
     3361 acatcctcgc acaatgaaac gactcgaaag gatccgctat gataagagca agagtaagga
     3421 tttgcatctg atgagatccc ttggatacct tgattttata gcgctgcaag ctaatgcaaa
     3481 attggttata acggattctg gaggaattca agaggaaact acctttctgg gagtaccttg
     3541 cttaacaatt cgagataaca cggaacgtcc tattacggta gatatcggaa ccaacacgct
     3601 tgttggaaga aatcttactc ggcttgagaa agaagttgag ctaattttaa cggatcaggg
     3661 gaaacgaggc caaatacctc ctctgtggga tggaaaagca agtcaaagaa tcgtacaagc
     3721 tctcagaaac caagcctaat atcaataaat gaagaattct ggcagaataa tttttgtttc
     3781 agaagtatac tacccagatg aacagggtac tgcttactac atcacaggat tagccgaggg
     3841 gctggcagag cactaccaac ttactgtttt ctgcggatat cctgccgtta cagcacgggg
     3901 aagaaaggta ctggccagag agacaataaa tggagttcgt gttgagcgct ctcacgggac
     3961 tacttttaat aaagacaaat tgctactgag actgataaac ctgggttccc tgagtttgtt
     4021 tatttttttc aagctcctca tttctgtgcg caaagacgat attgtaattg tggtgaacgg
     4081 accccatccg ttaccttatc tggccaggct ggtgtgtttc tttcataatg caaggtgtat
     4141 tttgcgcatc gatgacgtgt acccggaagt ttttatcgct actggaataa taccagaaaa
     4201 gagcattata gcgcgctgca taagatatat aaatacattg ctcttcaggg ggatggaccg
     4261 agttgttgtc ctggggcgtg acatgaaggc tctggcggaa aaggcaacca aaaacggtgc
     4321 caaccatatt gaaataattc ataattgggg agatgtggat actgtgcttc cgaaagataa
     4381 ggcaatcaat cccttgctgg cacaacttgg cctcaaagat aggtttgtaa tatcttgtgc
     4441 tggtaatatg ggaagggttc aggccataga gaccatgttt ggcgccatca atcggataaa
     4501 aaacaacgct gatatacact ttgtatttgt cggctctggc tcgaagagac cctggatgga
     4561 aagggaaatc agaacaaaaa aattactcaa cgttaccctc gttgaccaga gaccaaggtc
     4621 tgatcagata aattttctga atgcatgtga tcttgcgctg gcatccctca tacgcggaat
     4681 ggcaggggca ggtgtgccga gccgtatgta taatgtaatg gccgctggca aaccattgat
     4741 tgcaattgct gaagaagaat cagaattgtc aagagttgtg cgggaagagc agattgggtg
     4801 ggttgtagag ccagataacc ctgaaaagct tgcgaatgta atcctggaag catatatgaa
     4861 aaaagaagtt cttcataata tgggaaaaag ggcaagaata gcggcagaac aaaaatattc
     4921 aagaggcaag atcattcaag actaccgagg attattgcaa tctctcgact gttgagatat
     4981 agacagagca tctgattaag taactcattc caggcaataa tgatactgtt gaagcggcag
     5041 gacatgacca aggaagaaaa gccagggcca atacttcatt gaaaatattc ttcatattcc
     5101 tcttatcctt tacaacatct tttttcatta tgccttttat aatatattta gcccgcaggt
     5161 gtgattgtat tgatcatccg gggcaaagaa gcatgcacgc tgaccctaca ccacgatggg
     5221 gcgggctggc attaaccgta ggtatggtgc ctgccctctt ttttctggaa attgatcggc
     5281 aggtaatctc tttcgtcatt gcaattctca ttcttattgt tattggagga attgacgact
     5341 gtaaaaggct tggttggagg acaaagcttt ttgctattct gctttccata acaacctttg
     5401 tttttctcgg aggcgtatct atcagaagcc ttggtttcta tggacttgac tttaacgttc
     5461 ggcttggatg ggtgtctata ccctttactt atttttgtat cgttggtgtt acgaatgcaa
     5521 ttaaccttat tgatggtttg aatgggctcg ctggtggtat atcattcatg gcttttgttt
     5581 ttcttgggat tggggggatg atgtcaggag agcctgtgct tgggttcatc tgtttctctt
     5641 ttgctggcgc cctactcggt tttctttttt ataatttccc taaagcccga atttttatgg
     5701 gcgattcagg gagtctgctg attggctttt ctcttgcatc gctttcaatt ttgcttactc
     5761 aagggaacaa ccacattcat gcagtcggtc cgattattac cgaggttgac ctttacaagg
     5821 ccgccctcag tgaagagttt gccttacagc ctatgtttcc tgtgctaatc ctttttgtgc
     5881 ctatttttga taccctcaga gtgattacgg tcaggctcct tctgcgaaag aaccctttcc
     5941 atccggatag aagccatttg caccatttat tcattaagtc gggattctgc cctattacaa
     6001 cggttgttgc actctggact gtaacatttc tttgtggggt tgtcgctctt ctgcttataa
     6061 agcagtcatc ggtaccatat cttggcattg tacttggttt gtcccttttt gtaagttggc
     6121 ttgccgatgc cctgggaaga aaaagaaagg atcaacaaaa gcagttgaag gaggccaagg
     6181 atacatgatt gcgaaaaaag tattaagtgg tatgtcagtg ctgttactga tcatgttttc
     6241 catgcctgtg catgctatgg actcagaagt tacgagggag acacttaacg gccttccagg
     6301 gttttatgta gcattcgaag aattacagcc aaacatcaag cagtatgatg tcacagcatc
     6361 aatgaccaga gatcaactac aaaaagatat agaaatgagg cttcaaaggg caaagataac
     6421 agttctttct caggctcaat ggctgaatac acgtggcaga ccggtcctct acgggaacat
     6481 taatactcac ctcgaggacg caaatattgc ttacaatata cagcttgaag cccgccagat
     6541 agtatttacg gattcaaaca acagagtaaa aacactcgcc ggaacctggg gcattactat
     6601 gacaggaata acaaagattg ataaattgga agtcatcagg cagaatcttt taactctcgt
     6661 ggataaattt gttgaggcat attggacagc gaatggaaag gatagcaaga agtaagacgg
     6721 ggctgatggg aaagactgca aaaaaa
//
LOCUS       DAOOMC010000140         6736 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdus sp. isolate AMR_MDS_2685
            NODE_13488_length_6736_cov_5.837300, whole genome shotgun sequence.
ACCESSION   DAOOMC010000140 DAOOMC010000000
VERSION     DAOOMC010000140.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864760
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdus sp. (wastewater metagenome)
  ORGANISM  Syntrophorhabdus sp.
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae; Syntrophorhabdus.
REFERENCE   1  (bases 1 to 6736)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6736)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,729
            Genes (coding)                    :: 2,708
            CDSs (with protein)               :: 2,708
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 7 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6736
                     /organism="Syntrophorhabdus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13488_length_6736_cov_5.837300"
                     /isolate="AMR_MDS_2685"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2699756"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..358)
                     /locus_tag="PK125_11845"
     CDS             complement(<1..358)
                     /locus_tag="PK125_11845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB38835.1"
                     /translation="MREIKGVSAWVMGIFYVAFVGWSLYGAVTPIETYIFRMVHMGLI
                     FALAFPAYPMLKKSFPKGWIADVILAALGVATIVWALCDVDQFIRRSTLPDPPDFYFG
                     LIAIILLAELSRRVVGM"
     gene            complement(459..1106)
                     /locus_tag="PK125_11850"
     CDS             complement(459..1106)
                     /locus_tag="PK125_11850"
                     /inference="COORDINATES: protein motif:HMM:NF013053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HPB38836.1"
                     /translation="MFKADKSIVIAFCTLTVLSGLLFFDRPVVAQDVIAEWNSIKTPP
                     PPELKDVTVDPKTTAFLLLDFNKQTCNSERRPRCLTSIPKMKKLLDFARSKGILIVYS
                     LSAGAVPGDIATDLAPRAADPVITSGPDKFMGTSLEKILKDNGITTVIVTGTAAHGAV
                     LYTASSAALRGMQVIVPVDGISAENVFAEQYVVWNLVNAPRISTNVILTRAERIK"
     gene            complement(1292..2353)
                     /locus_tag="PK125_11855"
     CDS             complement(1292..2353)
                     /locus_tag="PK125_11855"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HPB38837.1"
                     /translation="MTLSPNPVEFFFQWHLTERCNLHCTHCYQEDGSIEEMSLSQAKE
                     VVNIARDTITSWSDTYEIAFSPSFNVTGGEPFLSPFLPDILSYLKTKEFDIYLLTNGT
                     MINRDRAHMLAGIPLQGVQVSIEGPEEIHEQIRGKGSFKKAIQGISTLLDAGIIVTLN
                     VTLSEINCNYVKDLVELASSVGAQRLGFSRLVPYGRGKGLISGMIGQDKLKQVYEDIL
                     SIKHTMVSITTGDPVASQIGVDPDYEHRDIPLGGCAAGVSGITILPDGTLTPCRRLGI
                     PIGNIFKDSFREIWATSPVLESLRIKTSYKGKCGICARWSQCRGCRAIAYAYSLAQGT
                     GDYLSEDPQCFITDSATKE"
     gene            complement(2658..6062)
                     /locus_tag="PK125_11860"
     CDS             complement(2658..6062)
                     /locus_tag="PK125_11860"
                     /inference="COORDINATES: protein motif:HMM:NF015380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="clostripain-related cysteine peptidase"
                     /protein_id="HPB38838.1"
                     /translation="MSELYRFSDDTQNILNKTRLPSGGFINLWLNTGHYEVTQEAAER
                     FGFSSAAVDILRDAAQDPDFYEFTLPSAHAQSPDKVSFMPQGPEKEIVVQESMGAYKQ
                     WVLDIVDKIHRAMQKSDIRIALYWIGYTLHGIQDLGPHGGIPNPEHAALDSQGQSPDK
                     DPNSVQLSRYYTHEFLRHIREVLGKDQFDLLRNWDGDGNLSKSEKTSLFGHGWDLSVK
                     AAMEFRKGGEKWLNMTPTPASVRWPRERVFTEILESLDVAVPQRRPGLKTKARKQAIE
                     APGFAFQTLGPAITPQPKAKWTVMVYMAGDDSNPNGIEYAVTQDLRELKDVGSCDSVH
                     LLAQTDQCREKNSYRYRLRKDTPLASDRLGVYRGDLNTGRVATLVDFVDWAVFNFPAE
                     RYALVLWGHGSGHDDQDVYRLARGDISPRLAARLAQDRIGFFGRTRRAMFERNGPNKG
                     FGFDSTAADFLDNRELKRAITSVVKKIGKPLDLLGFDACLMANMEVAYQLRGLVTWMA
                     ASEQTEPGDGWAYREALRGLAAKPDQDARQLGKAIVKSFGEAYDKQQTLSLVHVQKID
                     DLAKNLKGLAHVLKDQTPVFHRAATKATDLSPEGREGYRDLGAFLKALKNDTDSSIRE
                     ATSQCLKIYRQIIQAKTGPGSGLTIYLPGHYNPYLPGSTDALYQLMDFPMDCGWSELL
                     DKVNSPPGWDRATVSRFKLQSIQAAPCFGSLYGIDRHNAKEILQGIDTGTKVRHWVDL
                     FRRNLFSDLKNRVDDILEMLHRDDGPPQVRIFVLPGLMGSQLSDRDGIHGLVWLDPVG
                     LAIGSDFPALRLTPDGIHDADSGVKIEAAGPVPLIYDRLAVTLMAAFGRSVDYLAYDW
                     RKPVAICGSDVADRIAKKIGNSKNIPVILVAHSLGGLVAARAIEHLKQKYPAVHKCVK
                     GLVSMGTPWKGSFNGALAMCAQGKTVEQFAMLTKWDVREIADICQTFWGLSDTLPGDY
                     PELLNPEIYAPGPLASSPNAIQQLSSPKTLNIKPPVNTFAIVSDLFNTVNNLVPKKSG
                     FEIEEGPGDGTVPLWSATYGGTLESVTVIQKHMTLPMDGRAIKATIDRISDWTAIKPK
                     LLINTGDLKKVYPPLPLTRDDFVESLENMEDRPLPLETYTSLLFLG"
     gene            complement(6285..>6736)
                     /locus_tag="PK125_11865"
     CDS             complement(6285..>6736)
                     /locus_tag="PK125_11865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB38839.1"
                     /translation="PSSGSIGVKTGIFQWLRAGWSGINFVKPGHILSGMKRFARDCKT
                     IMESSRLPSSNQIVSVYQMLSNMTASELLKEYSGLKQAQRSLAIQTGKISKTEKEEKI
                     SFANTPKEQMIEELMLENLKISLGKPTVLGKQSSLLSTKSQLYISHQ"
BASE COUNT         1682 a         1568 c         1580 g         1906 t
ORIGIN      
        1 tcatgcctac aacccggcgg gaaagttcag ctaaaagtat aatggctatg aggccaaaat
       61 aaaagtcggg tggatcaggg agagtcgagc gccgtataaa ttgatctaca tcacacaacg
      121 cccatacaat ggtagctacg ccaagcgcag cgaggataac atctgctatc catcctttcg
      181 gaaaagattt ttttagcatg gggtaagctg gaaaggcgag tgcaaaaata agccccatat
      241 gtaccatacg aaaaatatac gtctcaatcg gtgtgacagc cccgtaaagg gaccatccca
      301 caaaagcaac ataaaaaata cccattaccc aggcgcttac accttttatt tcacgcaccg
      361 tttaaatcct ccttaagttg aacggaagaa taataacgaa ataaaggggt taatgtcaaa
      421 agatttaata catctgagta agggcaagcc tttctcgttt attttattct ttccgctctc
      481 gtcaaaatca catttgtcga aatacgcggg gcattgacaa gattccacac gacgtattgc
      541 tctgcaaaaa cgttttctgc agatataccg tccaccggta cgattacttg catgccccta
      601 agggctgcac tgcttgcagt ataaagcacg gcaccgtgcg cggcggttcc tgtaactatt
      661 actgttgtga ttccgttatc tttcaggatt ttttcgaggg atgtgcccat aaacttgtca
      721 ggtcctgatg tgatgacggg gtccgcagcc cgtggggcca ggtctgtcgc aatatcccca
      781 ggtactgccc cggcagacag gctatatacg atcaagattc cttttgagcg ggcaaagtcc
      841 agaagttttt tcatttttgg gatcgatgtt aaacagcggg gccggcgttc tgagttacag
      901 gtctgtttgt taaaatcaag caacaagaaa gcagtcgttt tgggatcaac cgtcacatct
      961 tttagctcgg gaggtggtgg tgtcttaatg gagttccact ctgctataac gtcctgagct
     1021 acgaccggtc tatcgaaaaa taacaggccg gataaaactg ttagagtaca aaaggcgatc
     1081 actatggatt tgtctgcttt gaacatgttt ttctcttctc ctgcttattc ttatatgaga
     1141 gctgctttac cattaaaaga gatatttata tgtttccctg ccatgagtct actgctctct
     1201 gtggtgacat ttcaattagt cctgaagcct tctcggctga tactggctgt ggctcgaaag
     1261 ccaaatggtg tatgtattga aattggtgat atcattcttt tgtggcagaa tcagtaataa
     1321 aacattgggg gtcctctgag agataatcac ctgtaccctg tgccagggag taagcataag
     1381 ctatggccct gcatccgcgg cattgtgacc agcgggcgca tatcccgcat ttgcctttgt
     1441 aagaggtttt aatacggagt gactcaagaa caggcgaggt tgcccagatt tctctgaatg
     1501 aatctttgaa aatattacct ataggaatgc caagccttct gcacggtgtg agtgttccat
     1561 ccggaagaat cgttatcccg gatacgccag cagcgcaacc accgaggggg atatctctgt
     1621 gttcataatc cggatcaaca cctatctgtg acgcaacagg atcacctgta gtaatagaga
     1681 ccatagtatg tttaatggaa agaatatcct catatacctg tttaagctta tcttgcccga
     1741 tcataccact gatgaggcct ttgcccctac catagggaac gagtcttgag aatccaagtc
     1801 gttgtgcccc cactgatgat gcaagttcca ccaaatcttt tacatagtta caattgattt
     1861 ctgaaagggt tacattgagc gtgacaatta tgcccgcatc caggagtgta cttataccct
     1921 gtatagcctt tttgaagctg cctttgcccc tgatctgctc gtgaatctcc tccggtccct
     1981 ctatgctaac ctgtacccct tgcaagggaa tacctgctaa catatgggcc ctatcgcggt
     2041 tgatcattgt tccgtttgta aggagatata tgtcaaattc ttttgtctta agataactga
     2101 gaatatccgg gagaaagggt gataggaatg gctcacctcc tgttacgttg aaacttggtg
     2161 agaaagcaat ttcgtaagta tctgaccaac tcgtgatggt atcgcgggca atgttaacga
     2221 cctcttttgc ttgagaaagg gacatctctt caatactgcc atcttcctgg taacagtgcg
     2281 tgcagtgcag gttgcaccgc tctgtcaggt gccattggaa gaaaaattcc acaggatttg
     2341 gcgatagtgt cataaaagat ttttcagata acgaggcaga taaggcgaag gccctatctg
     2401 cctgttgctg ttatgttgca gacctgcaac attcgggttt aaccctttca gctctttcac
     2461 ttgtgacatt tttttgctgt cgatttgata ttaattgttt taacgcccag ccagaatgca
     2521 gcctcctttt ccagaactcc cttttttaac accctcttca ccaccatcac cccctttcat
     2581 caaaaatgtt gatggaagca tcacatcaat aaaagaatag catagtttta aagcctgtca
     2641 agaaaaggtt ttcttaatta tccgagaaat aacaggcttg tatatgtttc taacggaaga
     2701 ggtctgtctt ccatattttc cagagattca acaaagtcat cacgtgtcag gggtaatggt
     2761 ggatatacct ttttcaaatc ccctgtattg attaaaagct ttggtttaat ggctgtccaa
     2821 tcggatattc tgtcaattgt ggcctttatt gccctgccat ccattggcag ggtcatgtgt
     2881 ttttggataa ctgtaactga ttcaagcgtg ccgccatatg ttgcgctcca cagaggcaca
     2941 gtgccgtctc caggcccttc ttctatttca aagccgctct tttttggtac aaggttgttc
     3001 actgtattga agagatcaga cacaatggca aaggtgttga ctggcggttt tatgttcagg
     3061 gtttttgggg aggagagttg ctgtatagcg tttggtgatg atgcaagagg cccgggcgcg
     3121 tagatttccg ggttgagtag ctcaggataa tcaccgggca atgtgtcaga aagtccccaa
     3181 aaagtctggc agatgtcagc aatctctctc acatcccatt ttgttagcat ggcgaactgt
     3241 tcaacagttt taccctgtgc acacattgca agtgctccat tgaatgatcc tttccatggt
     3301 gtacccattg acaccaggcc tttaacacat ttgtggacag cagggtattt ctgtttcaaa
     3361 tgctcgatag cacgggcagc cacaaggcca ccgagggaat gggcaaccag gataacagga
     3421 atgtttttag aattcccaat tttctttgct atgcgatcag caacatcact accacaaatt
     3481 gctacgggct ttctccaatc ataggcaaga taatcgactg atcgtccgaa tgcagccatc
     3541 agagtaactg ccagtctgtc gtagatgaga ggtacagggc ctgctgcttc gatttttact
     3601 ccagaatcgg catcgtggat tccgtcaggg gtgagccgaa gggcggggaa atctgaaccg
     3661 atagcaagac caacaggatc aagccagaca agtccgtgta tgccatcccg gtcgctgagt
     3721 tgagagccca tcaggccagg gaggacaaag atccttacct gaggaggccc atcgtcgcga
     3781 tgtagcattt caaggatatc atcaaccctg tttttcagat cactgaaaag atttctgcga
     3841 aacaggtcaa cccagtgtct cacctttgtg ccagtgtcga tcccctggag tatctctttt
     3901 gcgttgtgtc ggtctatacc gtacaagctg ccgaaacacg gggcggcttg gatggactgg
     3961 agtttaaatc tgcttactgt ggctctgtcc cagcctggag ggctgtttac tttgtcgaga
     4021 agttccgacc acccgcagtc catgggaaaa tccatgagtt ggtaaagggc atctgtgctg
     4081 ccaggtaaat acgggttgta gtgacctggt aggtagatgg tgagtccgga tcccggccct
     4141 gtctttgcct ggattatctg tctgtaaatc tttaggcact gtgaggttgc ctcccgtata
     4201 ctgctgtctg tatcattttt aagagctttc aggaatgcgc ccagatctct gtagccttcc
     4261 cggccctctg gtgagaggtc agtcgctttt gtagcggccc tatgaaagac aggggtttgg
     4321 tctttgagca catgagcaag ccctttaagg ttttttgcca gatcatctat tttttgaaca
     4381 tggacaaggc tcagggtctg ctgtttgtca taagcctcac cgaatgactt cacaatggct
     4441 tttccgagtt gtcttgcatc ctggtctggt tttgcggcaa gacctcttaa ggcctctctg
     4501 taagcccagc catccccagg ttcggtctgc tcgctcgcag ccatccatgt tacaaggcct
     4561 cttagctggt aggcaacctc catgtttgcc atgaggcatg catcaaatcc aaggagatcc
     4621 aatggtttgc cgatcttttt aaccactgat gttatggccc ttttcagttc ccggttgtca
     4681 agaaaatcag cagcggtact gtcgaaacca aagcccttgt taggcccatt cctctcaaac
     4741 attgcacgcc tggttctgcc gaaaaaccct atcctgtcct gggcgagacg ggcagcaagc
     4801 ctgggtgaga tatcaccacg ggcaagccgg taaacatcct gatcatcatg tccgcttcca
     4861 tgaccccaga gcacaagggc atacctttct gcaggaaaat tgaagactgc ccagtccaca
     4921 aaatcgacaa gagtggctac ccttccggta ttcagatctc cacgatatac ccccagacgg
     4981 tcagaggcaa gaggagtgtc ttttctaagc cggtaccgat atgaattctt ctcccggcat
     5041 tggtctgttt gggcaagtaa atggacactg tcgcatgaac caacatcctt aagctcccga
     5101 agatcctgtg tgacagcata ttctatccca ttagggttgg aatcatctcc tgccatataa
     5161 accattacag tccattttgc ttttggctga ggggttatgg ctggtccaag ggtttggaaa
     5221 gcaaacccag gcgcttcaat tgcctgtttc ctggccttgg ttttaagacc ggggcgtctc
     5281 tgtgggactg cgacatccag agattcgaga atttcagtga aaactcgttc acgtggccat
     5341 cgcactgagg ctggagtcgg ggtcatgttt agccatttct ctccaccctt tctgaactcc
     5401 atagctgcct tcaccgacag gtcccaccca tggccgaaga gagatgtttt ctcagacttt
     5461 gaaaggttgc catcaccatc ccagttgcga agaaggtcaa actgatcttt tcccaaaacc
     5521 tcacggatgt ggcgcagaaa ctcgtgggta taatagcggg aaagttgtac gctatttggg
     5581 tccttgtctg gactctggcc ttgtgaatcg agtgctgcat gctcggggtt tggtatgccg
     5641 ccatggggac caaggtcctg gatcccatga agggtgtagc caatccagta aagggctatc
     5701 ctgatatcac ttttctgcat ggccctgtga atcttgtcaa caatatctag cacccattgt
     5761 ttgtatgctc ccatactttc ttgaacaacg atttcttttt ccggtccctg cggcataaaa
     5821 gataccttgt ccggtgactg ggcatgggca gagggaaggg taaattcata gaaatcagga
     5881 tcctgggctg catcacgaag gatgtcaacg gctgccgatg agaaaccgaa ccgttcagca
     5941 gcctcctgtg tcacttcgta gtggccagta ttgagccaga gatttatgaa gcctcctgat
     6001 ggaagtctgg ttttattgag aatgttctgt gtatcatcag aaaaacggta taattcgctc
     6061 ataatcccct cctgaaaatc cgtaacgcgt aatccgtaac gcgtgataag tcaacatcag
     6121 aatatttcat cactatttac tctttttagt tgatagtcat acttccataa ttgtcgtgta
     6181 aatccaagtt gctttgcaac agaaaaaaca ccctgggctg ccttttttaa gtaactccgt
     6241 tgattaggcc ggggtacggg gataaggttg aagcaggtga aggcctactg gtgagaaatg
     6301 tacaactggc tttttgttga cagtaatgag gattgtttac cgaggacggt tggttttccc
     6361 aggcttattt tgaggttctc cagcatgagt tcctcgatca tctgctcttt aggtgtgttt
     6421 gcaaatgata tcttttcttc tttctctgtc ttgctgattt tgccggtctg aatggcaaga
     6481 gatcgttgtg cctgttttag gccactgtat tctttaagta attcgcttgc ggtcatatta
     6541 gagagcatct gataaactga tacaatttga tttgaagagg gtaaccggga tgattccata
     6601 atagttttgc aatcgcgggc aaagcgtttc atcccgctaa ggatatggcc tggtttaaca
     6661 aaattaattc ctgaccaccc ggctcgaagc cattgaaaga tacctgtttt tacaccaatt
     6721 gacccggaac tcggct
//
LOCUS       DAOOMC010000141         6526 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdus sp. isolate AMR_MDS_2685
            NODE_14094_length_6526_cov_3.436563, whole genome shotgun sequence.
ACCESSION   DAOOMC010000141 DAOOMC010000000
VERSION     DAOOMC010000141.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864760
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdus sp. (wastewater metagenome)
  ORGANISM  Syntrophorhabdus sp.
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae; Syntrophorhabdus.
REFERENCE   1  (bases 1 to 6526)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6526)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,729
            Genes (coding)                    :: 2,708
            CDSs (with protein)               :: 2,708
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 7 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6526
                     /organism="Syntrophorhabdus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14094_length_6526_cov_3.436563"
                     /isolate="AMR_MDS_2685"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2699756"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..534)
                     /locus_tag="PK125_11870"
     CDS             complement(<1..534)
                     /locus_tag="PK125_11870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB38840.1"
                     /translation="MADNAAKLFDEREKRISDAIALRVPDRVPILVFFGFFPARYAGM
                     TVQEVMYDPEKIWQAQTKTLFDFQPDGDQNPYLLRYLGPILETLDFKQLMWPGHGLTS
                     DHTYQFVEGEYMKAEEYDAFLSDPTDFIIRRYWPRVCGRLQGLEKLPPLHNIISYYMG
                     IPTGFGAFSLPEVVEALE"
     gene            complement(536..1165)
                     /locus_tag="PK125_11875"
     CDS             complement(536..1165)
                     /locus_tag="PK125_11875"
                     /inference="COORDINATES: protein motif:HMM:NF014375.2"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HPB38841.1"
                     /translation="MNTKLVELMSDLMEDETIALVKELIKGGTNPMDILDDARSAMEV
                     VGKRFETSEYFIPDLIMAGEILKEISEIVKPLLEKSGGSAKKGKVLIGTVAGDIHDIG
                     KDIVTFMLDVSGFDVLDIGIDVPVQTFIEKIKEFQPQVVGLSGFLTLAFDSMKKTVEA
                     IEKEGLRDKIKIMIGGGQMDETVKTYVGADAYGKDAVAGVQLCKQWIGG"
     gene            complement(1267..2460)
                     /locus_tag="PK125_11880"
     CDS             complement(1267..2460)
                     /locus_tag="PK125_11880"
                     /inference="COORDINATES: protein motif:HMM:NF024850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HPB38842.1"
                     /translation="MKRFFLGTILIIVGLLYIMSANSYAQQKPAPYPLGLVADLTGRM
                     SDMGTAIKRGMDIAIDNVNAAGGVNGRQIKAFVYDGESDPSKSVLHSKRLIEVDKAAI
                     LMGYNLSGATMASIQTAESGKTVLYSASASEKIWIPTKKWIYNVVPRQWEASIPMLIE
                     VLQQKGVKKIAYIYIDTTYGQTGKETFDRAVKEMKFTPAIVEKYAPGTTDVAPQITHI
                     KAAGADGILITGNLADTVMVIKNARDMGFTGPIVSDYAIVGPEFIQLGGKYVEGVVTT
                     SLKALVAPELPANDPQKKVAMALYNEYIKRHGSFSLYAGHMWDEIYLLVEALKKVDPK
                     LDPTKDADLIKIRAQLRDNLEQIKGFVGQNGIFNYSPDNHNGLPPRCYVPVVVEGGKW
                     RLYKK"
     gene            complement(2611..3387)
                     /locus_tag="PK125_11885"
     CDS             complement(2611..3387)
                     /locus_tag="PK125_11885"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPB38843.1"
                     /translation="MQIEANVSVCKLPGERTGEPSLVVDQVSTFYGEAQALKEVSLEV
                     RTHEVVAILGSNGAGKTTLLKTITGLLAPRKGTITFRKHKIDGIPTHEVIRKGIASVP
                     EGRELFRTMTVWDNLLMGTYSLHENERKEVLKGRLDVIFGLFPILKKRLKQKAETMSG
                     GEQQMLAVGRALMANPRLLVLDEPSLGLSPVLVAEMMGSLKKICSEMEVSLLLVEQNA
                     RAALKIADYVYVLERGEIVLSGACANVISSPTIQAAYLGG"
     gene            complement(3387..4172)
                     /locus_tag="PK125_11890"
     CDS             complement(3387..4172)
                     /locus_tag="PK125_11890"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPB38844.1"
                     /translation="MKKPQAESMLLEVKGLSKRFGGIKAVEDVSFGIQEKGIISMIGP
                     NGAGKSTFINVVSGVYMPDAGEIYFAGEDVAGLPAHTMASKGLSRTFQLEELFINLTV
                     LENVLVGCHTGGSSGILSCGFSLPSARKEERRLRDESMECLREVGIAEKACNTISQLP
                     LGERKLVGIARALAMKPKLLLLDEPAGGLAAHEVERLVQLIYTLKERGLTLFIVEHNM
                     PFVMSLSERIVVLDEGKKIAEGIPDEVKKNERVIKAYLGEEVA"
     gene            complement(4153..5142)
                     /locus_tag="PK125_11895"
     CDS             complement(4153..5142)
                     /locus_tag="PK125_11895"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HPB38845.1"
                     /translation="MKLMSKISVVWVFLIVAIIVVPLTSSSYIIGVMCFVAVFGALGI
                     GMGVLMEQAGIFSLAHPTWFGLGAYISGILAVQGVTPPWLGSIVGAVCVAILSVLIGA
                     PLLRLRGYYLACATFGLLLVVEISLAQLGSITGGHEGLMGIPPLTVFGFTFETDIHYY
                     YLSWGLCLACLWFLYNLMGSRVGRAVRAFSDSEIAAGSMGVNVPAFKLKIFVLTAVMA
                     SLAGSLYTFYLRFTQPGIFGFPLLVELMTMIIIGGGRTLYGPLLGSFVVLWLRELIHI
                     YLGKLLPRMTAEVDAIFFGVLIIIILVFMPGGLAGWVDRLLGFNRRVIEKATG"
     gene            complement(5150..6013)
                     /locus_tag="PK125_11900"
     CDS             complement(5150..6013)
                     /locus_tag="PK125_11900"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HPB38846.1"
                     /translation="MSLFSQIVQYAISGITGGSIYAIIGICWSVVYLITRVLNFTTGE
                     FVMLGGMLTWGFHSSGMGIFSSFLIAVIVTICIGIFLERTTIRPVRFPSEMTYMMITI
                     AAASVIKGIILLVWGSETRTIEPFFGNKIISVFGATFTSQALCILTLVTIITIGLSLF
                     LNHTLFGKALRASAINETGARLMGIDINRFRLFCFGLAGGLGAITGIVITPITFTGYN
                     IGMLTGLKGLVAAIVGGWTIPGTVAAALTLGLLEGFAAGFISAGLKDVVALLVMIIYL
                     ISIERWTIHRG"
     gene            complement(6249..>6526)
                     /locus_tag="PK125_11905"
     CDS             complement(6249..>6526)
                     /locus_tag="PK125_11905"
                     /inference="COORDINATES: protein motif:HMM:NF019498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HPB38847.1"
                     /translation="QYEALAYKKLGKNMEPYIITPAFDEKKVFSHEGEEFMFVLEGTH
                     EFVYGNKRYILEPGDSIYFDSSIPHTGRSIGEKPAKVLAVIYSYKRS"
BASE COUNT         1895 a         1606 c         1258 g         1767 t
ORIGIN      
        1 ctcaagggct tcgacaacct cggggagact aaatgctccg aagccggtgg gaatccccat
       61 atagtaactg ataatattgt gtaacggcgg aagcttttcc aagccctgca atcttccgca
      121 cacgcggggc caatagcggc gtattataaa atctgtgggg tctgataaaa aggcatcgta
      181 ttcttctgcc ttcatatact ccccctccac aaactggtag gtatgatcag atgtaagacc
      241 atgaccgggc cacataagct gcttaaaatc aagggtttca aggatggggc ccagatatct
      301 gaggaggtag ggattctgat cgccatcagg ctggaaatca aacagtgttt ttgtctgcgc
      361 ctgccagatt ttttccgggt cgtacatgac ttcctgaaca gtcatgcctg catatcgagc
      421 tggaaaaaac ccaaaaaata ccaggattgg taccctgtcc ggcactcgca aagctatggc
      481 gtcactgatt cttttttccc tttcatcgaa aagttttgcc gcattgtctg ccatgttaac
      541 ctccaatcca ttgcttacag agctgcacgc cggctaccgc atctttgcca taagcatctg
      601 cgccaacata ggtcttaact gtctcgtcca tctgtccacc gccgatcatg atttttatct
      661 tatcccgcaa accttccttc tcaatggctt caaccgtttt tttcatggaa tcaaaagcaa
      721 gggttagaaa accactcaag ccaacaacct ggggttggaa ctcttttatc ttctctataa
      781 atgtctgaac aggtacatca attccgatat caagaacatc aaatccactt acgtcaagca
      841 taaatgtgac gatatctttt ccgatatcat gaatgtctcc ggcaactgta ccgatgagaa
      901 ccttaccttt ttttgcgcta cctcctgact tttcaagaag aggtttaaca atctcggaaa
      961 tctctttcag aatctcccct gccataataa ggtcaggaat aaaatattca ctcgtttcga
     1021 accgcttgcc aacaacctcc atggccgaac gggcatcatc gagtatatcc atagggtttg
     1081 tacccccctt gataagctct ttcaccagag caattgtctc atcctccatc aaatctgaca
     1141 tcaattcaac aagtttagta ttcataacgt ccctccagaa gtcaggtaag ggacaatggg
     1201 ctataggcta taggcaaaac attttgtatt gtcttatagc ccataacctc ttgcccatcg
     1261 tcattattat tttttataca gtctccactt tccaccttca acgacaaccg ggacatagca
     1321 tcttggggga aggccattgt ggttgtcagg ggaatagttg aagatgccgt tttgtcctac
     1381 aaaaccttta atctgttcaa gattatcgcg taactgggct ctaatcttga taaggtcagc
     1441 atcctttgtg gggtctaact tggggtcaac ctttttaagg gcctcaacaa gcaggtaaat
     1501 ctcatcccac atatggccgg cataaaggct gaaagaaccg tggcgcttaa tgtactcatt
     1561 ataaagggcc atggcaacct tcttctgagg atcgttcgca ggaagttcag gggcaacgag
     1621 ggctttaaga gatgttgtaa caacaccttc aacatatttg ccaccaagct ggatgaactc
     1681 aggaccaacg atagcgtagt cgctcacgat aggacccgtg aaacccatat ctcgtgcatt
     1741 tttgattacc ataaccgtat cagcaagatt acccgtaata agaattccat cagcacctgc
     1801 agctttgatg tgggttatct gaggggctac atctgtcgtg cccggcgcat acttttctac
     1861 aattgcaggc gtgaatttca tctctttcac ggcccgatca aaggtttctt taccagtttg
     1921 tccgtaagtt gtgtcaatat aaatataggc tatcttcttg actccctttt gttgtaatac
     1981 ttctatcaac attgggatgg atgcttccca ctggcgcgga acaacattgt atatccattt
     2041 ttttgttggt atccagattt tctctgatgc tgaagcgcta tagagaacag tctttcctga
     2101 ttcagcggtt tggatcgaag ccattgttgc gcctgagagg ttgtaaccca ttaatatagc
     2161 cgccttatca acctcaatta gacgctttga gtggagaaca ctctttgagg gatcgctttc
     2221 accatcatac acgaatgcct tgatctgacg tccattgaca ccaccagccg cattcacatt
     2281 atcgattgcg atatccattc cccttttaat cgcagtcccc atatctgaca ttctgcctgt
     2341 aagatctgcg accaatccta atggatatgg tgccggtttc tgttgtgcat aactattagc
     2401 cgacataata taaagtaaac ccacaatgat caatattgtt cctaaaaaaa atcgtttcat
     2461 gatagccctc ctctttttta ttagctttca atatatagac cttggtattc agctaagtcg
     2521 ttcttttccg tgagcgctca ggatattacc aaagccctgt attccatatc aataactata
     2581 catactttag cctttcaact cataactgtg ctacccccct aaatacgctg cctggattgt
     2641 tggacttgaa atgacattcg cacaggctcc tgacagaact atctcacctc tttccaatac
     2701 atacacataa tctgcaatct ttaatgctgc acgggcattt tgttcaacaa gaagtaatga
     2761 cacttccatt tctgaacaaa tcttttttaa agaccccatc atttctgcca caaggacagg
     2821 agataagccc aacgatggtt cgtcaagaac aagaaggcgg gggttcgcca taagtgctcg
     2881 ccccactgca agcatttgtt gctcaccgcc tgacatggtt tcagcctttt gtttaagtct
     2941 ctttttaaga attggaaata aaccaaagat aacatccagg cgacctttta atacctcttt
     3001 tctttcattc tcgtggagag agtaagtccc cataagcagg ttgtcccaga ccgtcatggt
     3061 tctgaaaagt tctcttcctt ctgggacact agcaatgcct tttctgataa cttcgtgcgt
     3121 cggtatgcca tcaattttat gttttctaaa agtaatagtg ccttttcgag gcgcaagaag
     3181 cccggtaata gtcttcagaa gtgtcgtttt gccggcaccg ttacttccaa ggattgccac
     3241 aacctcatga gtcctaacct ccagagagac ttctttgagt gcttgggctt caccatagaa
     3301 agtgctcact tgatcaacca caaggcttgg ttctccggtt ctctcgcctg gaagcttaca
     3361 gacacttaca ttggcttcaa tctgcattat gccacctctt cacccaaata cgcttttata
     3421 accctttcat tttttttaac ctcatcagga atgccctcag ctatcttctt tccttcgtcc
     3481 agcacaacaa tcctttctga caaagacata acaaaaggca tattgtgttc cacaatgaaa
     3541 agcgtgaggc ctcgttcttt caacgtatag atgagttgaa cgagacgttc aacttcatgt
     3601 gccgccagac ctccagcggg ttcatcaagc aacagaagct ttggcttcat tgcgagtgct
     3661 cgtgctatgc caacaagttt tctctcgccg aggggaagct gagaaattgt attacaggct
     3721 ttttctgcaa tacctacttc cctgagacac tccatggatt catcacgaag acgccgttct
     3781 tctttccgag ctgagggtag ggaaaaacca catgagagaa ttcctgacga tcctcccgta
     3841 tgacatccca ccaacacatt ttctaacaca gtaagattta taaagagctc ctctagctga
     3901 aaagtccggc tcagcccctt tgaagccata gtgtgtgcag gaagacctgc aacatcttca
     3961 ccggcaaaat agatctcacc ggcatcaggc atataaacac cggaaacaac attgatgaag
     4021 gtcgatttgc ctgctccgtt aggccctatc atagaaatta tacctttctc ctgaatgccg
     4081 aaggagacat cttcaacagc ttttatacca ccgaagcgct tgctcaggcc cttcacttca
     4141 agaagcatgc tttcagcctg tggctttttc aattactctc ctattaaaac caagcaacct
     4201 gtctacccag ccagcgagcc caccgggcat aaaaacgaga atgatgatta taagtactcc
     4261 aaaaaagatg gcgtctactt ctgcagtcat tctcggcagt aactttccaa gataaatgtg
     4321 aatcaattct ctcagccaca acaccacaaa agaaccaaga agtggcccgt agagtgtcct
     4381 tccaccacct atgataatca tggtcattaa ttctaccaaa agagggaaac caaatatacc
     4441 gggttgagta aaacgaaggt aaaaagtata aagactacct gcaagactgg ccatgactgc
     4501 agtcaataca aaaatcttta gcttgaaagc aggaacatta acgcccatgc tacctgccgc
     4561 aatttcactg tcgctaaacg ccctcacagc tctgccaact cgtgaaccca tcaggttata
     4621 aagaaaccac aaacatgcca ggcaaagacc ccaggaaaga taataataat gaatatctgt
     4681 ctcgaaggta aacccaaaca cagtaagggg gggaatcccc attaatcctt catggcctcc
     4741 agtgattgaa ccaagctgag caaggcttat ttcaacaacg agaagaagac caaatgtggc
     4801 gcaggctaaa taatacccgc gaagccttaa caggggggct ccaatgagga cagataaaat
     4861 agcaacgcaa acggcgccaa cgatgcttcc cagccaagga ggtgtaacac cttgaactgc
     4921 caatataccg gaaatgtaag ctccaagtcc aaaccatgta ggatgggcaa gagaaaaaat
     4981 cccagcctgc tccatgagaa cacccatgcc gataccgagg gcaccgaata cagcaacaaa
     5041 acacataaca ccaatgatgt atgaacttga tgtgagagga accacaatga ttgctacaat
     5101 cagaaaaacc caaacaacag agatttttga cattagcttc atgaatactt catcctcggt
     5161 gaatggtcca tctttctatg gaaataaggt aaataatcat cacaaggagt gcaaccacat
     5221 ctttcagacc tgctgagata aaccctgctg caaacccttc aagaagaccc aaggtcagag
     5281 cagcagctac cgttccaggg atagtccacc cacctacaat tgccgctacg agtcctttga
     5341 ggccagtaag catcccgata ttatacccgg taaaggtgat aggcgtgatt acaatgccag
     5401 ttattgcgcc taagcctcca gccaaaccaa aacagaaaag acggaaacgg ttgatgtcga
     5461 tccccatgag ccgcgctcct gtctcattga tagccgatgc acgaagcgct ttaccaaaaa
     5521 gagtatggtt taaaaataag gataatccaa tagttataat agtgactagg gttaggatgc
     5581 aaagcgcttg tgaggtgaag gttgcgccaa atacactgat tattttattc ccgaagaagg
     5641 gttcaatagt cctggtctca gatccccaaa caagtaaaat aattcctttt atcactgaag
     5701 ctgcagcaat ggtgatcatc atataggtca tttctgatgg gaatcgaact ggccttatgg
     5761 tcgttctctc cagaaaaata ccaatacaaa tcgtaacgat gactgctatg agaaatgatg
     5821 aaaaaatccc catgccagaa gaatggaacc cccatgtcag catgcctccg agcatcacaa
     5881 attcaccagt tgtaaagttg agtactcttg ttattagata gacaacactc caacatatgc
     5941 cgatgatggc atagatgctc ccgcctgtta taccggaaat ggcatactgg acaatttgtg
     6001 aaaaaaggct cacccaaata accccctgaa tgatcgtaaa atatacaatc cgttgcttgg
     6061 gtaatgcaat aacaattacg cattaaccat cattttcatg atttgagatt tttttcaatt
     6121 ttacaaggtg ctgacaacca tcggacattt atttattggc agccctcaac aagagtcaaa
     6181 ctcggaataa aaactatagc cttgagtatt acttttctgt gatctttttt aaaaagtaaa
     6241 ttttatcatc atgatcgctt ataagaataa ataactgcaa ggacctttgc gggcttttct
     6301 ccaatactcc tgccggtatg gggaattgat gaatcaaaat aaatactgtc gcctggctca
     6361 agtatgtacc tcttgttacc ataaacaaac tcgtgcgtcc cttcaagcac gaacatgaat
     6421 tcttcgcctt catgggaaaa cacttttttt tcgtcaaacg ccggtgtgat aatatatggc
     6481 tccatatttt tcccgagctt tttatatgcc agggcttcat actggt
//
LOCUS       DAOOMC010000142         6407 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdus sp. isolate AMR_MDS_2685
            NODE_14455_length_6407_cov_5.176322, whole genome shotgun sequence.
ACCESSION   DAOOMC010000142 DAOOMC010000000
VERSION     DAOOMC010000142.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864760
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdus sp. (wastewater metagenome)
  ORGANISM  Syntrophorhabdus sp.
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae; Syntrophorhabdus.
REFERENCE   1  (bases 1 to 6407)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6407)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,729
            Genes (coding)                    :: 2,708
            CDSs (with protein)               :: 2,708
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 7 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6407
                     /organism="Syntrophorhabdus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14455_length_6407_cov_5.176322"
                     /isolate="AMR_MDS_2685"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2699756"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..276
                     /locus_tag="PK125_11910"
     CDS             <1..276
                     /locus_tag="PK125_11910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB38848.1"
                     /translation="SAIFGVLCEDSYPRKGNASTYGLNITVHITVELFIRDVTNATQR
                     VKWIAPEYPTPQLLLTNQRNAVAIVSLHSGTISTDTTSNRGFSYMKM"
     gene            675..2156
                     /locus_tag="PK125_11915"
     CDS             675..2156
                     /locus_tag="PK125_11915"
                     /inference="COORDINATES: protein motif:HMM:NF015669.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaY family lipoprotein"
                     /protein_id="HPB38849.1"
                     /translation="MKIILKSHIQYFMLVFIGLIASCNSTHFHGKNEDMSNLARGAII
                     EGTAIYRERIAIPPNSVFEAILEDVTIIDAKAIEIGQVSFRPSGGPPIRFSITFNPAR
                     IDPTRLYSVRAHILVNGRPMFASDRLHPVLTRGAGHTVDILLKMVGHNFNKSMPPDSI
                     TTSDLQTIGAHGLRLPATFLGDLPCFDCLAIRHHLDLWPDQVFHLRREWVGKNLIRDE
                     VGRWRVDPSRHALILHGGGETKAQFQIRPGNRLRQLDIKGAPIESTLPCELLDEGKFS
                     PIDLSLTLEGEMTYMADAARFTECLTGRSYPVAQEGDFKKIHRAYLKNASNPGAHLYV
                     TFDGSIIHSPRMEGASIERRVVVHRFIDARSNQKCKQYQTDASLTNTYWRITSLDGRP
                     VNPFNSWREPHILFISEDQRQTFAATVGCNQLVGTYTITGKNIAFNNTASTMMACPPI
                     LDTLEKYLNDIITRTKQWQLKDNVLEFIDDKGVQIALFEAVYL"
     gene            2311..3081
                     /locus_tag="PK125_11920"
     CDS             2311..3081
                     /locus_tag="PK125_11920"
                     /inference="COORDINATES: protein motif:HMM:NF013053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HPB38850.1"
                     /translation="MRLNVLFLVLIACSIAFFMTVTMSDIAQGAEKMVTVQAKPEPLK
                     IDLARTAVVVVDMQNAFASKGGMFDLVGQDISGAKSVIANNKQIITAARKAGIKVIYL
                     LMSYTPDLSNSGGPGSPNWYKEFGLAMMNKKPEYKGKFLIEGTWDEAVVDELKPEPGD
                     IVIRKMRYSGFRGTSLDVVLKTYNVKYLILTGIATNVCVESTLRDGYFLDYWPILVAD
                     ATNNAGPPITQQATLWNVEALFGWVTSTKDMVKAISGK"
     gene            3580..5676
                     /locus_tag="PK125_11925"
     CDS             3580..5676
                     /locus_tag="PK125_11925"
                     /inference="COORDINATES: protein motif:HMM:NF012200.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor D domain-containing protein"
                     /protein_id="HPB38851.1"
                     /translation="MLKNGRRLQLFILLSVIAFLLGAVHESSGAFRVIQSSPGRNTLY
                     VSSAHNHIVRDSTGKLYVAYSLQWHNYIRWSSDNGKTWSEPVNTETLPDSISIQSLVV
                     DTSDNLYQGFTFNVGSFFTKSTDGGVTWTPETQLHDGGWGAWDYWPSLIIDGSGTLHT
                     VYFAAFGWGDWPANVIYKTSSDNGDTWSPGMDLTGIPNLDNYGLGAQHPNMYAGSGNN
                     LFVLYSDYLTFTEPHLITKKLLHFNGTTWSSPLTVSSEDISMLTGDIAVDSSGLLHII
                     YHEKNSITQNYQILYRTYNPGTQDFSMPIALTPDSTNAYYSTMGIYSGDKVVIAYDTY
                     DNQSQKHAGVFLKDSSDSFVSTKRVSVHPEARWPNLRSSKYVMNQPNEMDLIWIEPNN
                     ITGGEDLVLFVKDSPEIAVYPDTINFESVLLNESLLQTVTITNMGLTDLVVDSITVDG
                     TSASMYSVSPGGATPCENLTPILTPDSSCTIQIIFTPTELGSQDAFLNINSNDNDMTP
                     LQISLTGKGVMALFTPSEGTIGTVLAITGSDFGEKKGKVLIGGYPTKIGKNGWTDTAI
                     TCTVNKPLPEGPHIVEIRPLRKEVIILSDAFVMKNPEITDDPPSSSGFTGEEITLNGN
                     FFGPKKGKVYFEYEQAGTIKNKSCKVKEWSMNKIVFVVPKTTRQFPADTYLLKVSNKV
                     GSVEVGEFTIYSGNPT"
     gene            complement(5897..6136)
                     /locus_tag="PK125_11930"
     CDS             complement(5897..6136)
                     /locus_tag="PK125_11930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB38852.1"
                     /translation="MRDNIEQYHALKRVEKRLPVVLCMEVGKTEICEVKNKAGVVEGD
                     AEDEVRISYRYVVIGDNKKTDGLGESEGNENGNGP"
     gene            complement(6181..>6407)
                     /locus_tag="PK125_11935"
     CDS             complement(6181..>6407)
                     /locus_tag="PK125_11935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB38853.1"
                     /translation="VTWTSGGIINKFGSNIRTCLMSCVYLKWYGPIASCEIYETRTKV
                     CRSYVPGSSNLCPQYNEVRQVGKEINRVTD"
BASE COUNT         1749 a         1483 c         1365 g         1810 t
ORIGIN      
        1 agtgccatat tcggagtgct gtgtgaggat tcctaccccc gtaaaggaaa tgcatcaacg
       61 tacggtctta acataacagt tcatatcaca gtcgaattgt ttatcaggga tgtcaccaac
      121 gctactcaaa gggttaaatg gattgcaccg gaatatccaa caccacagtt actcttgaca
      181 aatcagagaa atgcagtggc aatcgtatcc ttacactccg gtaccatctc gacagacact
      241 acaagtaacc ggggcttctc ttatatgaag atgtgaacga agattttttt ctccctataa
      301 tacatcccct tccagtaagg tctcaatatc ccagattcca gggtgggaat ctatcttttg
      361 ctgagttttt ttttaaaatt aaacaacatt tttagtgaac aatcgcgttc tttgtgtgcg
      421 gtatctggtt ttttcctgcg ttgtggaaat gggggaaaat ttttctctac cctccatgat
      481 tatgtcatac tatgaattat caaaatatcg tagtaggttg tatcgttgta aaaggaacct
      541 ttattgcttt ccagttcgca aattcaccgt cctccgtcca tgtactccac ctttgacttc
      601 accaactcat gacgttaata tagaaaatct ggaagcatat ggtgactgag tatggataat
      661 atgaagggtt ccttatgaaa atcattctta aatcacatat acaatacttc atgttggtat
      721 tcattgggct gattgcgagc tgtaacagca cccattttca tggcaagaat gaagacatgt
      781 caaacttggc aagaggagct attattgaag gcaccgcaat ttacagagag cggatagcta
      841 ttccaccaaa ctcggttttc gaggcaattc ttgaagatgt gacaattatt gatgcaaaag
      901 ccattgagat cgggcaggtt tcgtttcgcc cttctggagg ccctccgatc cgcttctcaa
      961 tcacttttaa tcccgcaagg attgacccca ctcggctcta ctcagttaga gcacacattc
     1021 tggtaaatgg aaggcccatg tttgcttcgg ataggctaca tcccgttttg actcgtggtg
     1081 ccgggcatac ggtggatatt ctccttaaga tggtgggaca caattttaac aaaagtatgc
     1141 cacccgacag tatcacaacc agtgatttac agaccattgg agcgcacggt ctgcgactgc
     1201 ctgcaacttt tctcggagat cttccgtgct ttgattgtct ggcaattcga catcaccttg
     1261 acctctggcc ggatcaggtg ttccatctca gacgggaatg ggtaggaaag aatctgattc
     1321 gtgatgaggt cgggagatgg cgcgtagacc cctcccggca tgcgcttatc ctgcatggtg
     1381 gcggggagac aaaggcccag tttcaaatca ggccaggaaa cagactcaga caacttgata
     1441 taaaaggcgc acccattgaa tccactttac cctgcgagtt gctcgacgag ggtaagtttt
     1501 cccccatcga tctatcccta acattggaag gagagatgac gtacatggcg gacgcagcgc
     1561 gtttcactga atgcctcacc ggtcggagct accctgttgc tcaggaaggt gattttaaaa
     1621 aaatccacag agcctacctt aagaatgcca gtaatcccgg tgcacacctt tatgtaacct
     1681 ttgatggatc aatcatacac agcccccgga tggagggcgc cagtattgag cgccgtgtgg
     1741 ttgtgcacag gttcatcgat gcccggtcga accaaaaatg caagcagtac caaacagacg
     1801 cttcactcac aaacacatac tggcgtatta cgagccttga tgggcgccct gtcaacccgt
     1861 tcaacagttg gcgcgaaccg cacattcttt tcataagtga agatcagcgt cagactttcg
     1921 ctgcaactgt cggttgtaat caactggtgg gtacttatac tatcaccggc aaaaacatag
     1981 cctttaacaa tactgcctct actatgatgg catgcccgcc aatactcgac accctggaga
     2041 aatatttaaa tgatataatt accaggacga agcaatggca actcaaagac aatgtccttg
     2101 aatttatcga tgacaaaggt gttcagatag cactatttga agcagtgtat ctgtgagttt
     2161 tttttgccac cctgcccttc cagccctcct gcctcattat ttctcaattt ccactccccc
     2221 gcctgcctac ttgactcctg atcatggata attgtatcgt tttgttgaat tcctgggagc
     2281 agggagcgtc tattttatgg aggtgcttga atgagattaa atgtattatt cctggtattg
     2341 attgcctgtt cgatcgcgtt ttttatgacc gtaaccatgt ctgatattgc acagggtgct
     2401 gagaaaatgg taacagtaca ggctaaacct gagccgctta agatagatct ggcgagaaca
     2461 gcagtggtag tggtcgatat gcaaaacgcc ttcgcatcca agggcggcat gtttgatctt
     2521 gtaggccagg acatctcagg tgctaagagt gtgatcgcta acaacaagca gattattact
     2581 gctgcacgga aagcagggat caaggtcata tacctgctga tgagctacac acctgatctt
     2641 tcaaatagcg gtggacctgg ttcaccaaac tggtataagg aattcggcct tgccatgatg
     2701 aataagaaac cggaatataa aggaaagttc ctcatcgaag gtacgtggga cgaggccgtt
     2761 gtggatgaac tgaagccaga accaggtgac attgttatac gaaaaatgcg ctacagcggc
     2821 tttagaggca ccagtcttga tgtggtcttg aaaacatata atgtaaaata tctgattctt
     2881 actggtattg ccaccaatgt gtgtgtggag tctacgttga gggacggata ttttctcgat
     2941 tactggccaa ttctcgtggc agatgcaacg aacaatgcag ggcctccaat aacacaacag
     3001 gcaactttgt ggaatgtgga agctcttttt gggtgggtta cgtcaacaaa ggatatggta
     3061 aaagctatat ccggcaagta aaaacgacga ttccttgctc ccaggaatac cgataccttt
     3121 ccaactcgtc acatgggtac tgcaatcata aaaacaaaac tgataaactg gagtgccaca
     3181 aaacatataa tgaagaaata gagaattgcg acaaccaggc gataatgatc agatgtacag
     3241 attttttcat catagatacc ttattttttt atccttactt cacatggtta tggcttctct
     3301 ttctttcgac atgagttgat ttacctttaa tttcacagaa aatatgacga aacattacac
     3361 agtctgcttt gacctgcttg ccaagtgggt agatgaggtc gtccaaacgt tctgcatgca
     3421 gttaaacaaa aagtttttgc tgtagcttaa ccttcgtggc atttttgcga ttaactcata
     3481 ggtggcggtt acagtactta cccgccaatc tgttatatac cattacatat tggactcgga
     3541 tacgagggat atcaaaaaaa ttaaaacgga ggtttttgta tgctaaaaaa tggcagaagg
     3601 ttgcagttat tcattcttct ctcggttatc gctttcctgt tgggtgcggt gcatgagtct
     3661 tctggggctt tccgtgtaat tcagagttct ccaggtcgta atacgctgta tgtatcatca
     3721 gcacataatc atatagtgag agattccaca ggcaaacttt atgtagctta cagccttcaa
     3781 tggcataact acatccgctg gagcagtgac aatggaaaaa catggtcaga acccgtgaat
     3841 accgagacgc ttcccgattc gatcagtatc cagagccttg ttgtagatac atcagacaac
     3901 ctctatcagg gtttcacctt caacgtgggt tcctttttta ccaaatcgac agacggcggc
     3961 gttacctgga cacctgagac acagcttcat gatggaggtt ggggggcgtg ggactactgg
     4021 ccttccctga tcattgacgg atctggaaca ctccacacgg tttattttgc tgcttttggc
     4081 tggggggact ggccggcgaa tgttatttat aaaacatctt ctgataatgg ggatacctgg
     4141 agccctggca tggatcttac tggcattccc aatttggaca attacggact gggagctcaa
     4201 catcccaaca tgtatgccgg gtccgggaac aacctgtttg ttctctactc agactatctt
     4261 acctttaccg agccacatct tataaccaag aaactccttc atttcaacgg tacaacatgg
     4321 tcgtcacccc tgactgtctc atcagaagac attagcatgt tgactggaga tattgctgtc
     4381 gattccagtg gattactcca cattatttat cacgaaaaga atagcatcac ccagaattac
     4441 cagattttgt acagaaccta taacccaggc actcaagact tctcaatgcc cattgcgctc
     4501 acaccagact caacgaatgc atattattca acaatgggaa tctattcagg tgataaggtt
     4561 gttatcgcct atgataccta tgataatcaa agtcagaagc acgcaggtgt atttctcaag
     4621 gattcctcag acagctttgt gtctacaaaa agggtttctg ttcatccaga ggcgagatgg
     4681 cccaacctca ggagctcaaa atatgttatg aaccagccta atgaaatgga tctcatctgg
     4741 attgaaccaa acaatattac tggaggggaa gacctggtcc tttttgttaa agatagtcct
     4801 gaaattgctg tttatcctga cacaatcaat ttcgagtctg tattgttgaa tgaatcttta
     4861 ctccaaacag tcacaattac aaatatgggg ttaacagatc tcgttgttga ctcaatcacc
     4921 gttgatggta cttcggcttc aatgtattcc gtaagtcctg gcggagcaac tccctgcgaa
     4981 aacctcacgc ccattcttac cccggattct tcctgcacca tccaaatcat ctttacccct
     5041 actgaacttg gctctcagga cgcgtttctc aacatcaact caaacgacaa cgatatgact
     5101 ccactccaga tatctttgac tgggaaagga gtaatggctt tgtttacacc ctctgaagga
     5161 actatcggca cggtgctggc cattactggt tcagacttcg gcgaaaagaa gggtaaggtc
     5221 ctcatagggg gttatcctac caagattggc aagaacggtt ggactgatac tgcaataacc
     5281 tgcacagtaa acaagcctct gcctgagggc cctcatattg ttgaaatcag gccattgagg
     5341 aaggaagtaa taattttatc agacgctttt gttatgaaaa atccggaaat caccgatgat
     5401 ccaccatctt catccggctt caccggtgaa gagattactt taaatggaaa ctttttcggt
     5461 cccaaaaaag gtaaagtata ctttgagtat gagcaggctg gcacgatcaa gaataagagc
     5521 tgcaaggtca aagagtggtc gatgaacaag atagtctttg ttgtgccaaa aacaacaagg
     5581 caattcccgg cagatactta tctactaaaa gtctccaaca aggtggggtc agttgaggta
     5641 ggagaattca caatttacag cggcaatcct acttaggagg cattaataac aaaggcagta
     5701 acttacaggg gctgagtttt tattctcagc ccttttcttt gtgtcccttc aatataacat
     5761 tgcgtcatgc acttaaggtt gtttttagtc aataaagggc atattgtaac cctccctcgt
     5821 ggatcaatta gcttttatgg caactttgtt gcttacccag ggtcgttcaa aatctgattc
     5881 atgcctgatt accggtttaa gggccattgc cgttttcatt gccttcactc tcacccaaac
     5941 catccgtttt cttgttatcc ccaatcacaa cataccggta gcttatcctt acctcgtcct
     6001 cggcatcacc ttccacaaca ccagccttat tcttaacctc gcatatttca gttttgccga
     6061 cttccataca aagtactact ggcaatctct tttcgactct tttcaatgcg tggtactgtt
     6121 ctatgttgtc ccgcaagtct cctttccttt tttcccgccc tgctctccta tctttacccc
     6181 ttaatctgta accctgttta tttcctttcc cacctgtctc acctcattat attgcgggca
     6241 cagatttgat gaaccgggaa catagctccg gcacaccttt gttcgtgttt catagatttc
     6301 acatgaagcg attggcccgt accattttag atatacgcag ctcatgaggc aggtcctgat
     6361 atttgacccg aacttattga tgatcccccc ggaagtccac gttacgt
//
LOCUS       DAOOMC010000143         6373 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdus sp. isolate AMR_MDS_2685
            NODE_14543_length_6373_cov_6.230769, whole genome shotgun sequence.
ACCESSION   DAOOMC010000143 DAOOMC010000000
VERSION     DAOOMC010000143.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864760
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdus sp. (wastewater metagenome)
  ORGANISM  Syntrophorhabdus sp.
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae; Syntrophorhabdus.
REFERENCE   1  (bases 1 to 6373)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6373)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,729
            Genes (coding)                    :: 2,708
            CDSs (with protein)               :: 2,708
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 7 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6373
                     /organism="Syntrophorhabdus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14543_length_6373_cov_6.230769"
                     /isolate="AMR_MDS_2685"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2699756"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..356)
                     /locus_tag="PK125_11940"
     CDS             complement(<1..356)
                     /locus_tag="PK125_11940"
                     /inference="COORDINATES: protein motif:HMM:NF013856.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein"
                     /protein_id="HPB38854.1"
                     /translation="MARDRVDVVLAKKGLASSREKAKALIMAGQIYVNEERVSKPDKL
                     VAEDACIEVKGNSIPYVSYGGIKLEHALEIFGIDVINKSALDIGSSTGGFVDCLLQHG
                     ASHVYAIDVGTHQLHER"
     gene            complement(338..2200)
                     /gene="dxs"
                     /locus_tag="PK125_11945"
     CDS             complement(338..2200)
                     /gene="dxs"
                     /locus_tag="PK125_11945"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: protein motif:HMM:NF003933.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HPB38855.1"
                     /translation="MFLEKINTLKDLRDMDPAEMNQLAGEIRQYLTEVVSRTGGHLAS
                     NLGVVELTIALHYVFNTPEDKIIWDVGHQCYTHKILTERRNRFETLRQDGGISGFPSR
                     KESPYDVFDTGHASNSISIAVGLSEATKKSHNNSKIIAVIGDGSLTGGMAFEALNHAG
                     HLKSGIIVVLNDNEMSISKNIGALSAHLNKIMTGEFVSNIREEIKGRIKNLPGFGNRV
                     YKAARHLEEVIKGLITPGMLFETLGFTYVGPVDGHNLNHLLDNLKNIRRLKGPVLIHV
                     ITKKGRGYNHAEDNPARFHGISTFEVTTGMCKASACTYTDVFGDTIIELARKDEKIVA
                     ITAAMGLGTGLDRFSSLFPDRFYDIGIAEQHGITFAAALALGGYRPFVAIYSTFLQRA
                     YDQIILDVCLQNLPVVFAVDRSGIVGQDGPTHHGVFDISYFRHIPNVTVMSPKDENEL
                     RHMLYTAYLLQKPAVVRYPRGETQGVIMDETFKEIPLGRWERLKEGKDIAVIACGNTV
                     WPALSAAVELESHGIHCCVVNGRFIKPMDGEMLVALASEVPRVLTVEENVLAGGFGSA
                     VMEFFSEEGITIPVKRLGIPDVFLPHGSQYNLRKSIGIDKEGIKRFIQKWLGTA"
     gene            complement(2200..3084)
                     /locus_tag="PK125_11950"
     CDS             complement(2200..3084)
                     /locus_tag="PK125_11950"
                     /inference="COORDINATES: protein motif:HMM:NF012568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HPB38856.1"
                     /translation="MDLNKYLLDKKILIENSLKDIALSFHDCPSPLRDAMEYTLFSNG
                     KRIRPILAITTCEAMGKSCDDILPFACAIEMIHSYSLIHDDLPTMDNDDMRRGKATCH
                     KAFGEATALLAGDALLTEAFRVMSDNRYTERTNSRITKQLIFEISSAAGAMGMVGGQV
                     MDVLSEGKEGTRDVLHYIHTHKTTALLRASVRVGAIFAGAKVRELKKLTKYGESVGLA
                     FQIRDDLLDAEGEEMKVGKRLKKDSTKQTYMKHYGPIASKIRLEQLVEDAVQAVQFLG
                     RDSIILAEIARFVGNRVA"
     gene            complement(3084..3305)
                     /gene="xseB"
                     /locus_tag="PK125_11955"
     CDS             complement(3084..3305)
                     /gene="xseB"
                     /locus_tag="PK125_11955"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR01280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HPB38857.1"
                     /translation="MKFEEGLKKLEGIVNTLGDGKIALDEALNLFKEGLSLTKELSRR
                     LEEIEKKVEVLIKKEDGSYEKAKYPQEET"
     gene            complement(3349..4002)
                     /gene="yqeC"
                     /locus_tag="PK125_11960"
     CDS             complement(3349..4002)
                     /gene="yqeC"
                     /locus_tag="PK125_11960"
                     /inference="COORDINATES: protein motif:HMM:TIGR03172.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium cofactor biosynthesis protein YqeC"
                     /protein_id="HPB38858.1"
                     /translation="MGAGGKTSLIEYFASRLVKEGKTVAITTTTKIFVREPYQLLKNS
                     KDQIDVTSLNRFGRTLENGKLTAVNDEDIQRIGSSYDIVLIEADGAKHKPLKFPAPYE
                     PVVPSVAEKIYVVSGLDALFHQVQEVVFRYELFCNITGIDGQAVVTEDVFMNFFSPSI
                     LFKGVDTGKCTIVLNKYDVLRQRSCGGIVAKGLLKNIHGPDLIVSSINHHVFYRITH"
     gene            complement(4043..4294)
                     /locus_tag="PK125_11965"
     CDS             complement(4043..4294)
                     /locus_tag="PK125_11965"
                     /inference="COORDINATES: protein motif:HMM:NF020967.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2007 domain-containing protein"
                     /protein_id="HPB38859.1"
                     /translation="MSGEKWKKIHVIENIFEMDMIKDALEKEDIEYTIREYKDTAYDG
                     LFVLQKGYASLLVREKDVESAKSIVKHIRSIPYVVFSKD"
     gene            complement(4291..5103)
                     /gene="folE2"
                     /locus_tag="PK125_11970"
     CDS             complement(4291..5103)
                     /gene="folE2"
                     /locus_tag="PK125_11970"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: protein motif:HMM:NF010200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003933 - GTP cyclohydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0035998 - 7,8-dihydroneopterin
                     3'-triphosphate biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase FolE2"
                     /protein_id="HPB38860.1"
                     /translation="MIDVQSMNDGRMIEIDKVGVKNIKYPIVVLDKVNGLQHTIATID
                     MYVNLPHHYKGTHMSRFVEILHTNKSLINMKDFPNILREMKYRLNAEAAHLSIRFPYF
                     IKKEAPVSKIESFMEYQCGMFGSMDATDKMKEFIVSVAVPINTLCPCSKEISEFGAHN
                     QRGITKVSVRFKRFFWIEDLITIVEKCASSDIYSILKREDEKFVTEHAYENPKFVEDV
                     VRDIAETLSKDANITWFTIEAENFESIHNHSAYAFLERNNIAREPDKTDEKK"
     gene            complement(5224..5592)
                     /locus_tag="PK125_11975"
     CDS             complement(5224..5592)
                     /locus_tag="PK125_11975"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: protein motif:HMM:NF013411.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070497 -
                     6-carboxy-5,6,7,8-tetrahydropterin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase"
                     /protein_id="HPB38861.1"
                     /translation="MFTLCVKDSFSAAHRLEDYHGKCEELHGHNFVVEALFSGEKLSS
                     GGMVIDFKILKDHLENVLKSLDHTYINDISFFNQRAGSSEYIAMYVYDELNRAIKNGP
                     VQLLEVKIWESETAWVSYRE"
     gene            complement(5708..>6373)
                     /gene="rplC"
                     /locus_tag="PK125_11980"
     CDS             complement(5708..>6373)
                     /gene="rplC"
                     /locus_tag="PK125_11980"
                     /inference="COORDINATES: protein motif:HMM:TIGR03625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HPB38862.1"
                     /translation="YDFFGGGKMGLGLLGKKLGMTQIFDDHGNAVPVTVIKTGPCKVI
                     QKKTSLKEGYDALQLGFEEVQKIGRVNKPMTGHFKKASAAPVKFLKEFRVTPEDVNER
                     EIGSEITVDIFEPGEFVDVTGTSKGKGFAGVIRRHGFKGQPASRGTHEFFRHGGAIGQ
                     NMTPGRTFKGTKMPGHMGNRRVTVQSLKIVDVRGDTNILLVEGAIPGPTNGYVIVKKA
                     VKK"
BASE COUNT         1613 a         1492 c         1264 g         2004 t
ORIGIN      
        1 cgctcatgta actggtgggt tcctacatca attgcatata catgagatgc tccgtgttgc
       61 aggagacagt caacaaaccc acctgttgag gatccgatat cgagagcgct tttatttatc
      121 acgtctatgc caaatatttc gagtgcatgc tccagtttta ttccgccata gctcacatat
      181 ggtatagaat ttccttttac ttctatgcag gcatcttctg caacaagttt atccggtttt
      241 gatacacgct cctcgttgac atatatctgc cctgccatta taagcgcttt tgccttctct
      301 cttgaagagg cgaggccttt tttcgccagg actacatcta cgcggtccct agccactttt
      361 gtataaaccg tttgataccc tccttatcga ttcctatcga ttttcttagg ttgtattgag
      421 agccgtgagg gaggaatacg tctggtatgc ccagtctttt gaccgggatt gtgatgcctt
      481 cttctgagaa aaactccatt actgcactcc cgaagccacc tgcaagaaca ttttcctcca
      541 ctgtgagtac acgtggtacc tcacttgcca gggcaaccag catttcccca tccatgggtt
      601 tgataaatct accatttaca acgcaacagt ggataccatg actctctaac tccacggcag
      661 ctgaaagcgc aggccacaca gtattcccgc acgcaatgac tgcaatgtcc tttccctctt
      721 tcaagcgctc ccatctgcca aggggtattt ccttgaatgt ctcatccatg ataacccctt
      781 gggtctctcc tctaggatat ctgacgacgg ctggtttttg aagaagatat gctgtgtaaa
      841 gcatgtgtct cagttcgttc tcatcctttg ggctcattac tgtcacattc ggtatgtgtc
      901 tgaaatatga tatgtcgaag accccatggt gcgttgggcc atcctgtccc acaataccac
      961 tcctgtcaac agcgaagaca acaggaaggt tctgaagaca aacatcgaga ataatctggt
     1021 catatgccct ttgaagaaaa gttgaataga tggcaacaaa gggacggtaa ccacccaggg
     1081 ccagtgcggc cgcaaaggtt attccatgct gttcggcaat cccaatgtca taaaatctgt
     1141 ctggaaagag acttgaaaac cgatcaagac cagtaccaag acccattgca gccgtgatcg
     1201 ctacaatctt ctcatccttt ctggcaagct ctataatggt atctccaaaa acatcagtat
     1261 atgtgcatgc actagccttg cacatgccag tcgttacttc aaaggtggag atgccatgga
     1321 atcttgctgg attatcttct gcgtgattgt atcccctccc tttttttgtg atgacatgga
     1381 tcagtactgg accttttaac cttctaatat ttttcagatt atctaagagg tggttcaggt
     1441 tgtgaccatc aactgggccg acataggtga atccgagggt ttcgaaaagc atgcccggtg
     1501 tgatgagacc cttaataacc tcttcaaggt gccttgcagc tttgtagacc ctgtttccaa
     1561 agccaggaag atttttaatt cttcctttta tctcctcccg tatgttgcta acgaattccc
     1621 cagtcattat cttgttgaga tgggctgaaa gggcgccgat atttttagat atagacatct
     1681 cattatcatt aaggacgaca ataatcccac tctttaaatg acctgcgtga ttaagggctt
     1741 cgaaggccat cccacctgtc agcgacccgt caccaatgac agcaataatc ttgctgttgt
     1801 tatgtgattt tttcgtggct tctgataatc ctacagctat agatatggaa ttgcttgcgt
     1861 ggcctgtatc aaaaacatca taaggacttt ccttacgaga gggaaaacca ctgatacctc
     1921 catcctgcct gagggtttca aacctgtttc ttctttctgt caggatttta tgggtatagc
     1981 actgatggcc aacatcccag attatcttat cttccggggt gttaaaaacg tagtgcaatg
     2041 caatggtcaa ttcaacaacg ccgagattgg aggcgagatg gccccctgtt ctggagacca
     2101 cctcagtaag gtattggcga atctcacccg ccagttggtt catctcagca gggtccatgt
     2161 ctcttaaatc ttttaaagtg ttaatttttt ccaggaacat taggcgaccc tgttgccgac
     2221 aaaccgtgca atctctgcga gtattattga atcccttccc aaaaactgga ctgcctgaac
     2281 agcatcttca acgagctgtt caagtctgat tttggaagcg attggaccgt aatgcttcat
     2341 gtaggtctgc tttgtgctat ctttttttaa gcgttttcca actttcattt cctcaccttc
     2401 agcatcgaga agatcatccc ttatctgaaa agctaggcca actgattccc catattttgt
     2461 aagttttttt agctcgcgaa cctttgctcc agcaaagatc gctccaaccc ttacagacgc
     2521 cctgagcaag gctgttgttt tatgggtatg gatataatga agaacgtctc tggtcccttc
     2581 ttttccttct gagaggacat ccatgacttg tcctccaacc atccccattg caccggctgc
     2641 tgatgatatt tcgaagataa gctgttttgt tattcgagaa ttagtccttt ctgtataacg
     2701 attgtcagac attacgcgga aagcctctgt gagaagcgca tcaccggcca gaagggcagt
     2761 ggcctcgcca aaagctttgt ggcacgtggc ttttcccctt cgcatatcat cattgtccat
     2821 agttggcaag tcatcgtgga tgagtgaata ggaatggatc atttcaatgg cacaggcaaa
     2881 gggcaaaatg tcatcgcagc tctttcccat ggcctcacat gtagtgatcg caagaattgg
     2941 ccttatcctc ttcccattgg agaaaagcgt atattccatg gcatctctca aaggagaggg
     3001 gcaatcatga aaagatagcg ctatatcttt cagagaattt tcaataagta ttttcttgtc
     3061 gagcaggtat ttgttaagat ccactatgtt tcttcctggg gatattttgc tttttcgtat
     3121 gatccgtcct cttttttgat caatacctca actttttttt caatttcttc gagccttctg
     3181 gaaagttcct tggtaagaga aaggccttct ttgaaaaggt ttaacgcctc atcaagagct
     3241 atcttcccat caccaagggt gtttacaatt ccctcaagtt tttttagccc ttcctcaaat
     3301 ttcatgtgcc tttattataa aatcgaaggt taattgtttg caagtggttc aatgggtaat
     3361 cctgtaaaag acatgatggt tgatacttga gacaatcagg tccggtccat gaatattctt
     3421 taacaggcct ttggctacta ttcccccgca gcttctttgt ctaagaacat catatttatt
     3481 taatacaatg gtacatttcc ctgtatcaac tcctttgaaa aggatggatg gtgaaaagaa
     3541 attcatgaag acatcttctg tcaccacagc ctgaccgtca atcccggtta tattgcagaa
     3601 tagttcatat cgaaagacca cctcttgtac ctgatgaaag agtgcatcta acccacttac
     3661 aacatagatt ttctcggcta cggatggaac aacaggttca tatggggcag ggaatttgag
     3721 tggcttgtgt tttgcgccat ctgcctcgat gaggactatg tcatacgatg agccaatgcg
     3781 ctgaatatcc tcatcattca ccgcagtcag ctttccgttt tcaagggttc tcccaaacct
     3841 gttcagggat gtaacatcta tttgatcttt actgtttttt agtagctgat aaggttcacg
     3901 tacaaatatt tttgttgtag ttgtaatggc gactgttttg ccttccttta ccagcctcga
     3961 cgcaaaatat tcgataaggc ttgttttgcc acctgctccc acaaaggcca catatttaaa
     4021 gggaatcaca gattgtacag ggtcaatctt ttgaaaatac cacataagga atggaacgaa
     4081 tatgtttgac tatggatttg gcagattcta catctttttc tcttaccagc aggcttgcat
     4141 atcccttttg cagaacaaaa agtccatcat aggcagtatc cttgtactct ctgatggtgt
     4201 attcaatgtc ttctttttca agggcatctt ttatcatatc catttcaaag atattctcga
     4261 tcacatgaat ttttttccac ttctcaccgc tcattttttc tcatctgttt tgtccggttc
     4321 tcgtgcaata ttgttccttt caaggaaggc atatgcgctg tggttgtgaa tactttcgaa
     4381 gttctctgct tcaattgtga accatgtgat atttgcatcc tttgacaagg tttcagcaat
     4441 atcccggacc acatcttcaa caaactttgg attttcgtaa gcatgctcag taacaaattt
     4501 ctcatcttct cttttcagga tggaataaat atcactgctt gcgcatttct ccactatagt
     4561 tatgagatcc tcgatccaga agaatctttt gaagcgaaca ctaaccttgg tgattccccg
     4621 ttggttgtgt gctccaaatt cactaatctc cttggaacat gggcaaagag tgtttattgg
     4681 aacagctact gagacgataa attctttcat tttatccgtg gcgtccatgg agccgaacat
     4741 accgcactgg tactccatga agctctcaat ctttgaaaca ggcgcctctt tttttatgaa
     4801 ataagggaaa cgaatgctaa gatgtgctgc ctcagcgtta agtcgatatt tcatctccct
     4861 caaaatattc gggaaatcct tcatattgat caggctcttg ttggtatgga ggatttcgac
     4921 gaaacggctc atgtgtgtac ctttgtagtg gtgagggagg ttgacataca tatcaattgt
     4981 ggcaatggta tgctggaggc cgtttacttt atctaaaaca acgatgggat attttatatt
     5041 tttgacaccg accttgtcta tctcgatcat ccggccgtca ttcatacttt ggacgtcaat
     5101 cataaatata tttctgccct tggccgcaac actcctgaaa catgttcatc gatttatact
     5161 tttgccgtct ggattttttt ccttctaagt ccacaagccc ctgaactctg gaaccttgtt
     5221 ttttcactcg cgataggata cccatgcagt ttcagactcc catatcttta cttcaagtaa
     5281 ctgtactggc ccgtttttaa ttgccctgtt cagctcgtca tagacgtaca tggcaatata
     5341 ttcagaagaa ccggcccttt gattgaaaaa agagatatca ttaatgtaag tgtggtcaag
     5401 gcttttcagt acgttctcga gatggtcttt gagaatctta aaatcgatca ccatgccgcc
     5461 actactcaac ttctcccctg aaaaaagggc ttctacaaca aaattatgtc catggagttc
     5521 ctcgcacttc ccatgataat cttcgagcct gtgggcagca gaaaaagaat cttttacaca
     5581 aagagtaaac atgttggtag tctatgttta tgggttattg aatgtcaacc ttctcgtttg
     5641 aagttccgaa tacgaaacat tatgtatgaa aaaagcccgg tactgtcctg taaccgttga
     5701 gttattgtta ctttttaaca gccttcttga cgattacata tccgtttgtc gggcctggga
     5761 tggctccttc gaccagaagg atattcgtgt ctcctctgac gtccacaatc ttgaggctct
     5821 ggacagtaac cctcctgtta cccatgtggc ccggcatctt tgtgccttta aatgttctgc
     5881 cgggtgtcat attttgaccg atagcgccgc catgtctaaa aaactcgtgg gttcctcgag
     5941 atgctggttg ccctttaaaa ccgtgtcttc tgataacacc agcaaatccc tttcctttag
     6001 atgtacctgt aacatctaca aactcgccgg gttcaaagat gtcaacagtg atctctgatc
     6061 ctatttccct ttcattcaca tcttcagggg ttacacgaaa ctccttgagg aacttaaccg
     6121 gagctgcaga tgcttttttg aaatgtccag tcatgggttt gttgacgcgg ccaatctttt
     6181 gtacctcttc aaatccaagt tgaagtgcat cataaccttc cttaagggag gtcttttttt
     6241 gaataacctt acatggtcca gtctttataa cagtaaccgg cacggcattt ccgtgatcat
     6301 caaatatctg tgtcatgccg agtttttttc cgagaagtcc aagtcccatt tttccacctc
     6361 caaaaaagtc ata
//
LOCUS       DAOOMC010000144         6256 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdus sp. isolate AMR_MDS_2685
            NODE_14875_length_6256_cov_6.201742, whole genome shotgun sequence.
ACCESSION   DAOOMC010000144 DAOOMC010000000
VERSION     DAOOMC010000144.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864760
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdus sp. (wastewater metagenome)
  ORGANISM  Syntrophorhabdus sp.
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae; Syntrophorhabdus.
REFERENCE   1  (bases 1 to 6256)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6256)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,729
            Genes (coding)                    :: 2,708
            CDSs (with protein)               :: 2,708
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 7 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6256
                     /organism="Syntrophorhabdus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14875_length_6256_cov_6.201742"
                     /isolate="AMR_MDS_2685"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2699756"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..568
                     /locus_tag="PK125_11985"
     CDS             <1..568
                     /locus_tag="PK125_11985"
                     /inference="COORDINATES: protein motif:HMM:NF019339.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPB38863.1"
                     /translation="YRTMAEKSLSLAAQYDPSLLRSPLVSLALASIYSEIGDKDEART
                     VLRESDDMAVLDPGFLVKKWALHFELGDAQRPIAQWGALFRGDKLTEADFTTLTVEMA
                     KQTIPDFRYFEAQILMQRGEMETARQKMSSCVSERPHVAEYRLALGDIYEKLGKRSDA
                     LTQYEKALKLSPANQYAKSKMIEYYKER"
     gene            600..2462
                     /locus_tag="PK125_11990"
     CDS             600..2462
                     /locus_tag="PK125_11990"
                     /inference="COORDINATES: protein motif:HMM:NF014740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate sugar
                     epimerase/dehydratase"
                     /protein_id="HPB38864.1"
                     /translation="MINKLLSVKRTLAMRRWFYFVGDSSCITLAIYVAFLLRFDWKIP
                     GAYNLLLYIPSILLIKLLVFWFFNLHNMSWTHTGPHEVVTITKANIVGSALVFLVIHV
                     FNPAQFFTGFPRSIFVADFVLCVFFITGFRLLKRLYLHYTKGSFAFGKQTLIVGAGDA
                     GVLLVKDMRHQNETTWAPLGFVDDDPGKQGIDVCGLRVLGKTNDIPELVEKLKIESIL
                     VAIPSAPSKVIQRIMEEIRKTPVRDIRVVPGVKRILEGTVTVSDIKEIAIEDILGREE
                     ATVDTESIRKMLSGKVLLVTGAGGSIGSEVVRQMIKYEPRKVVALDIDETDLFNLQQE
                     LIQKSGGENVVPVVADVGDWTKIQRIFEAFSPGIVLNAAAYKHVPMMESYPEEAVRVN
                     VLGTMNLLNASIKYHVERFVMISTDKAINPSSVMGATKRTAEELVRFYNSKNHARFIS
                     VRFGNVVGSRGSVIPIFQKQIVSGGPVTVTHKDMKRYFMSIPEAVILVLQAAASGTGG
                     EIFLLDMGEPVRILTVAEEMIRLHGLAPDKDIPIIFTGMRPGEKLYEDLMTPLEKSEQ
                     TAHPKIFKAINDTIKTDIIKQVALLEKAAVEGDKPRIIAILKEIIPAYNPQLHP"
     gene            complement(2428..2559)
                     /locus_tag="PK125_11995"
     CDS             complement(2428..2559)
                     /locus_tag="PK125_11995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB38865.1"
                     /translation="MYDDGNATGFRRDYALERLAKEAVEKRLPEKWFKGATGGYMRG"
     gene            complement(2611..3252)
                     /locus_tag="PK125_12000"
     CDS             complement(2611..3252)
                     /locus_tag="PK125_12000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB38866.1"
                     /translation="MKKVSILIGIAVALFFVMGSYNVQAQNLSALDGTWLKMTVKPQK
                     GLEFTGYDSTSLPRKMSARGGSLYVCMDVDVNAPHVAYLRFFEKDGTPMGDGLLHWDA
                     GTNLQFLGYLEASLGVDVDYLAGEDGFPAEGSLTDTYLKGYVNVAGKTADKIKITSIS
                     GEGWIQSPGATETEGTYAGFGYTLKGSITKGKKTPMILPSCGAMVFSENNHVE"
     gene            3537..>6256
                     /locus_tag="PK125_12005"
     CDS             3537..>6256
                     /locus_tag="PK125_12005"
                     /inference="COORDINATES: protein motif:HMM:NF013736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HPB38867.1"
                     /translation="MTGQPGTDTDNPNAQSLLQIILDISRSLISLSPDQIDEGIDDAL
                     KAIGEFVRADRVCIFRFSPDGQEMSNTHEWCGRDTEPQMHTLQGIATSDFPWFYEKIS
                     RGEVVHVPDVFQLPPEATAERTRWEAQNIRSLISIPIVCRDTLAGFIGFDAVSAIRPW
                     PEEIITLLKVVGEILASALEGKHSIELLKESEGRYKALFENANDAIFLLRENTFVDCN
                     NEALRMFGCAKDEIIGQPPFKLFPPTQPDGKNSKEKALKKIYAALFGYQQRFEWQHCC
                     LDGTPLDTEISLNRVELGGKTFVQAIIRDITKQKIAEAALKESEEKYRSIFENAVDGM
                     FQTTPDGRYISANPSLAKMYGYDSAEELTAAITDLRKKYVDPEDRDRLKASIEEKGYV
                     EGFETQMFRKDGTKMWISMTARAVRDESGDIVCYEGTSIDITARKEMEQLLERERETF
                     YGILHKAPFGAALIGKDGRYIFVNPEFTSLTGYTIEDIPTGKEWFNKAYPDEKYRHYV
                     IEEWKKDIGSRGIIKNFTIACKDGIAKEFEIRTAFLDDGQAVMMLVDITEQKRMEAAR
                     RESEDYLKTIFNYMPTGMIIIDPEDHTIVDVNSTAASMIKADVMSIVGKECHNYICPT
                     ERNQCPITDLGKTIDNSERLIKAADGQMISVVKTVIPISVKGHRYLLESFIDITERKQ
                     FEQTLKESEEKYRSLFEGSRDAIYIAASDGQLVDANQAFLDLFGYTREEIIHSNAKQA
                     YASVKDQKIFKQAIQEKGSIRDFAIQLRKKDGTIMDCLLSVTTKRGDDGNIIEYNGIV
                     RDISAYKKAQEMIHYMAYHDPLTGLPNRVLFNDRLDMALARAQRSGKKVAVMMLDIDK
                     FKSINDKWGHETGDKLLKSVADRIGHVLRKSDTIARMGGDEFLVIVPETE"
BASE COUNT         1776 a         1431 c         1517 g         1532 t
ORIGIN      
        1 ctaccgtacc atggcagaaa aaagcctctc ccttgcagct cagtatgacc cgtctcttct
       61 tcgcagcccc ctggtcagcc ttgcgcttgc ttcgatttac agcgagatag gggataagga
      121 cgaggcacgg acagtgttgc gggaatctga tgatatggca gttttggacc ccgggttcct
      181 cgttaagaaa tgggcgctcc acttcgaact cggcgacgct caaaggccaa tagcacaatg
      241 gggggcactc tttcgtggag ataaacttac tgaagccgat tttacaactc ttaccgttga
      301 aatggcaaaa cagactatac ctgattttcg ctattttgaa gcacagatac ttatgcagag
      361 aggggagatg gagacagccc gccaaaaaat gagctcctgc gtatcggaac ggcctcatgt
      421 ggcagaatac aggcttgccc tcggcgatat atatgagaag cttggaaaac gcagcgatgc
      481 tcttacccag tatgaaaagg ctctgaagct ctccccggct aaccagtacg caaaatcaaa
      541 gatgatcgaa tactacaaag agcgatgaag tgcaaaggat acaataagga ggtgaattta
      601 tgataaataa actcctgtcg gttaagcgta ctcttgctat gaggcgctgg ttttacttcg
      661 tcggggactc atcttgcatt actctggcaa tttatgtggc tttccttctt cgttttgact
      721 ggaagatacc cggtgcctac aatctgttac tgtacatccc ctcaatcctg cttattaagc
      781 tgctggtttt ctggtttttc aatcttcaca atatgtcatg gacccacacg ggcccccatg
      841 aggttgtgac aattacgaag gctaatatag tgggctccgc tctggtcttc ctcgttatcc
      901 atgtgtttaa tccagctcag tttttcacgg gttttccccg ttctattttt gtagcagact
      961 ttgttctttg cgtcttcttc ataacaggtt tccggttgct caaacgcttg tatttgcact
     1021 ataccaaggg gtcattcgcg ttcgggaagc agaccctcat tgttggggca ggtgatgctg
     1081 gagtgttgct cgtcaaggat atgcgccacc agaatgagac tacatgggct cccctggggt
     1141 ttgttgatga cgatcccggt aagcagggaa tagatgtctg tggacttaga gtgctgggga
     1201 agacaaatga cattcccgaa ctggtagaaa agctgaagat cgaatctatc cttgttgcta
     1261 taccatctgc gccgagcaag gtgatccagc gcatcatgga ggagatacga aaaacaccgg
     1321 taagggatat ccgcgttgtt ccgggcgtga aacggatact cgaaggcacc gttactgttt
     1381 cagatataaa ggagattgcg attgaagata ttctgggccg tgaggaggct actgtggata
     1441 ctgagagcat aaggaagatg ctcagcggca aggtgctgct tgtcaccggt gcaggaggtt
     1501 ccataggaag tgaagtggtc aggcagatga tcaagtacga acccaggaaa gtggtggccc
     1561 ttgatattga tgagaccgac ctgttcaacc tgcaacagga gcttatccaa aaatccggcg
     1621 gtgagaatgt cgtgcctgtt gtggccgatg tcggagattg gacaaagatc cagcggatct
     1681 ttgaggcctt ttcgccaggg attgtcctga atgctgccgc atacaaacac gtccccatga
     1741 tggagtccta tcccgaggag gcagtacggg taaatgttct cggaacaatg aacctcctga
     1801 acgcatcaat caaatatcac gtcgaacgct ttgtcatgat ctcaacggac aaggcgataa
     1861 acccgtcgag tgttatgggt gcaacaaaga gaacagcaga agaactggta agattctaca
     1921 atagcaaaaa ccacgcccgt tttatctccg tacggttcgg aaacgtggtg gggagcaggg
     1981 gaagtgttat ccccatcttt caaaaacaga tcgtttcggg tgggccggta accgtgaccc
     2041 acaaagatat gaagcgctac tttatgagta tcccggaggc tgtgatcctg gtgctccagg
     2101 ctgccgcatc aggtacaggg ggcgagatat tccttctcga tatgggcgag cctgttcgta
     2161 ttcttacggt ggcggaagaa atgatccggc tccacgggct tgcaccggat aaggatatcc
     2221 ccatcatctt cactggtatg cggccgggag aaaagcttta cgaagacctc atgacgccct
     2281 tggagaagag cgagcagact gctcacccga agatcttcaa ggcaataaac gacacaatca
     2341 aaaccgacat catcaagcag gtggcgctcc tcgaaaaggc cgctgtagaa ggtgacaaac
     2401 cacgcatcat cgccatccta aaagaaatca tccccgcata taacccccag ttgcaccctt
     2461 gaaccatttc tctggcagtc tcttttcaac tgcctctttc gccaatcttt ccagcgcata
     2521 atcccttcga aaaccagtag catttccgtc atcgtacata aaaatcccgc ctgcaaggct
     2581 gccctgcagg cgggagctga aaaaaatcaa tcattctaca tggttgtttt cagaaaacac
     2641 cattgcaccg caggaaggca aaatcatagg agtctttttg ccttttgtaa tgcttccttt
     2701 caacgtgtag ccaaagccgg catacgtgcc ttctgtctcg gttgccccgg gtgactgaat
     2761 ccagccctcc ccggaaatcg atgtaatctt tatcttgtca gctgttttcc ctgccacatt
     2821 cacatagccc ttcaggtagg tatcagtgag actaccttca gcaggaaagc catcctcccc
     2881 agccaaataa tctacatcga cacccagaga tgcctccaaa tagcctagaa attgcaggtt
     2941 ggttcctgcg tcccaatgaa gcaaaccatc ccccattggc gttccgtctt tttcgaagaa
     3001 cctcagatat gccacgtgtg gtgcattcac atcaacgtcc atacaaacat agagactccc
     3061 gccacgggcg ctcatctttc tggggagaga agttgagtca tacccggtga attcgagtcc
     3121 tttctgtggt ttgactgtca tcttcagcca tgttccatcg agggcactga ggttttgtgc
     3181 ctggacatta taactaccca taacaaagaa gagagctaca gcaattccga ttaaaattga
     3241 aacctttttc atatgatacc tccttgtaaa tgattatatc caatcttata cttttccgag
     3301 gttctctgca acaccttttt gccgcagcgc gcagatatat atggcatcgc ataggttctc
     3361 tgtctccatc agccatgggt atactggaga gtcccgggat tgttacaatt cttaagtaat
     3421 tattgcccca ttccgaatag aatatataaa cgcaaatact tatgggtact tgtgcaatta
     3481 aaaggacaaa agtcgggcca gggagtataa aacccgacga ttttttcgaa ggcaaaatga
     3541 ctggccagcc tggaacagac acggacaatc cgaatgcaca atctttgctt caaattatac
     3601 tggacatatc caggagtctc atcagtcttt cacctgacca gatagatgaa ggcattgatg
     3661 atgcgctgaa agcaattggc gaatttgtcc gggccgacag ggtttgcata ttccggtttt
     3721 ccccagacgg acaggagatg agcaatactc acgaatggtg tggccgggac acagagcccc
     3781 aaatgcacac actccagggt atagctacaa gcgactttcc ctggttctac gaaaaaatca
     3841 gccgtggtga agttgttcat gtccctgacg tgttccagct tccgcccgag gccacggctg
     3901 agagaacaag gtgggaagca cagaacatcc gctcactcat cagtattcct attgtgtgca
     3961 gggatactct tgcgggattt atcgggttcg acgctgtcag cgcgataaga ccatggcctg
     4021 aagagataat aaccctgttg aaggtggtag gcgagatatt ggcatccgct cttgaaggaa
     4081 agcattccat agagctttta aaagaaagcg agggaagata taaagcactc tttgaaaatg
     4141 ccaatgatgc catttttctt ttgagagaaa atacctttgt tgactgcaac aacgaggccc
     4201 tcagaatgtt cggctgtgcc aaggacgaga ttattggtca acctcctttc aaattatttc
     4261 cgccaaccca gcctgatgga aaaaattcca aagaaaaagc ccttaaaaag atttatgctg
     4321 ccctgttcgg ctatcaacag cgcttcgaat ggcagcactg ttgccttgac gggacccctc
     4381 tggatacgga gataagcctg aaccgggttg agctgggcgg taagaccttt gtccaggcaa
     4441 taatacggga tattacaaaa cagaagatcg ctgaagctgc tttaaaggaa agcgaggaaa
     4501 aatacaggag catttttgaa aatgcagtag atggaatgtt tcagacaacg cccgacggac
     4561 gttatataag cgcaaacccc tcacttgcaa aaatgtatgg ctatgattct gcagaagaat
     4621 tgactgctgc catcacagat cttagaaaga agtatgttga tcccgaagac cgagacaggc
     4681 tgaaagcttc tatcgaagag aaaggatatg tcgaaggttt tgaaacacag atgttccgga
     4741 aagatggaac aaaaatgtgg atttccatga cagccagggc agttcgtgat gaatcaggag
     4801 atattgtttg ttatgaaggg acttcaatag atattactgc gcgaaaggag atggaacagc
     4861 tccttgaaag ggaaagggaa acattttacg ggatacttca taaggctccg ttcggtgctg
     4921 cccttattgg caaagatggc aggtacattt ttgtaaaccc tgagtttact tctcttaccg
     4981 gttacaccat tgaagacatt cccacgggaa aggaatggtt caataaagct tatcctgacg
     5041 aaaaataccg acactacgtt attgaagaat ggaagaaaga catcggatcc aggggcatca
     5101 ttaaaaactt tacaatagca tgtaaggatg ggatagcaaa ggagttcgaa ataaggacag
     5161 cgtttctcga cgatgggcag gctgtcatga tgctggttga cattactgag cagaagcgga
     5221 tggaagcagc gcgaagggaa agtgaagatt atttaaaaac aatctttaat tacatgccaa
     5281 caggcatgat catcattgac ccggaagatc acaccattgt cgatgtcaac tccactgctg
     5341 ccagcatgat caaggctgat gtcatgagta ttgttggtaa agaatgtcac aattacatat
     5401 gtcctaccga acgcaatcaa tgcccgatta ccgatcttgg gaaaaccatc gataattcag
     5461 aaaggctcat taaagctgcg gatggtcaaa tgatatcggt tgtgaaaacc gtaatcccta
     5521 tttctgttaa aggacatcga taccttctgg aaagttttat tgacattacc gagcgcaaac
     5581 agttcgagca aaccttaaaa gagagcgagg agaagtaccg gtctcttttt gaagggtcaa
     5641 gagacgccat atacattgct gcatcagacg ggcaactcgt agatgctaac caggcctttc
     5701 tcgatctttt cggatacaca cgggaggaaa tcattcattc gaacgcaaaa caggcctatg
     5761 cttcggtgaa agaccaaaaa atcttcaaac aggccataca ggagaagggt tcaataaggg
     5821 attttgcaat acagctcaga aaaaaagatg gcaccatcat ggactgtctc ctctctgtga
     5881 ccacaaaaag aggggatgat ggaaatatta ttgagtacaa cgggattgtc cgcgacataa
     5941 gtgcctataa gaaggcccag gaaatgatcc attacatggc ttaccatgat cctctcaccg
     6001 gccttcccaa cagggtgctc ttcaacgacc gtctggacat ggccttagca cgtgcacaga
     6061 gaagcggaaa gaaggtggca gtcatgatgc tcgatatcga caaatttaag agcattaacg
     6121 ataaatgggg tcacgaaaca ggagataaac tgttgaaatc tgttgcagac cgtatcgggc
     6181 acgtcctgag gaaaagcgac acgattgcaa ggatgggagg agacgagttt cttgttattg
     6241 ttcctgaaac ggaaag
//
LOCUS       DAOOMC010000145         6156 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdus sp. isolate AMR_MDS_2685
            NODE_15215_length_6156_cov_6.249795, whole genome shotgun sequence.
ACCESSION   DAOOMC010000145 DAOOMC010000000
VERSION     DAOOMC010000145.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864760
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdus sp. (wastewater metagenome)
  ORGANISM  Syntrophorhabdus sp.
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae; Syntrophorhabdus.
REFERENCE   1  (bases 1 to 6156)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6156)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,729
            Genes (coding)                    :: 2,708
            CDSs (with protein)               :: 2,708
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 7 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6156
                     /organism="Syntrophorhabdus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15215_length_6156_cov_6.249795"
                     /isolate="AMR_MDS_2685"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2699756"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            125..1336
                     /locus_tag="PK125_12010"
     CDS             125..1336
                     /locus_tag="PK125_12010"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012336.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006635 - fatty acid beta-oxidation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HPB38868.1"
                     /translation="MKTFTKAYIPYNGYYSTPFTRWQGSMQNENAIELGAKTARRWFL
                     EKKKIDPTVIDYLYFGISIHQHHLFYSHNWAAGMLTDNKKNVPGLMVNQACTTSTTIL
                     SLLAKDIELGGYDVGFGLMADRCSNGAHTVWPNPMGPGGEVIHENWMMDNFNSDPNVK
                     PPTKMVGTAENVARLEGFTKEQSDAVVLRRYEQYQMALADDRAFQKRYMFPAEVKAGK
                     KGTKLVELDEGATPTTAEGLAKLKPAEPGGIHSFGAQTFPADGNVGFIVTNRDKAKEL
                     SADPKIEIQIISYGYSRVNPGFMAAAPVPAAQMALANAGLKITDMKAIKSHNPFTTND
                     LNFAKKMGIDVMFMNNYGSSLIYGHPQAPTAGRIIAEMLEEMAMIGGGYCLWAGCAAG
                     DTGASMIFKVG"
     gene            1912..2529
                     /locus_tag="PK125_12015"
     CDS             1912..2529
                     /locus_tag="PK125_12015"
                     /inference="COORDINATES: protein motif:HMM:NF012774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HPB38869.1"
                     /translation="MNRLMTAILLVLAIAYVNPLFGKEYVVKKVIDGDTVQLESGETV
                     RYIGINAPELYMKEGGSEFFAKEAARFNKKLVFMKKVRLEFDKEKQDSHGRMLAYVFV
                     KNIFVNAELVKHGYAKVMIKPPNTKYKDVLLAQQQKAVEEVRGLWQEKRQDTETTYIG
                     NKRTYTLHRPSCTYVKKIPDKSRITFRSRSDAIKIGYTPCRYCKP"
     gene            2526..4079
                     /locus_tag="PK125_12020"
     CDS             2526..4079
                     /locus_tag="PK125_12020"
                     /inference="COORDINATES: protein motif:HMM:NF024793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HPB38870.1"
                     /translation="MSEQYIEYWGLTQHPFLLAPDSKMMCVTGQYFECLERLKYAIYT
                     NKGGVLIVSEDAGLGKTTILLKLIDEMKDEYGDAFRFAFVDQPALSPAQMIAQITGSI
                     TKAEPHEDKLKNLKMLRDALLEANEHGGKSIIVVDEAQMLCEAHDVLQELRTLINLTH
                     NGQYLHTFILSGQRALWNTIKGMPEFWQRLPVRYYFIPLRVEETKEMIRFRLAKAGLE
                     EGREIFDDDSLEIIHRFSRGSPRTVIALSDLALLVGYTNRSAKITFKEVSKAINTMSG
                     KGETLPFMKTEKREGMPSLSALSNLDRGTEIGHIQEGAGVGGIRGREGWQMWFMQYGQ
                     YVRPLIALLVITALILLIIMGFRYVFFQKKESDVAEIAKQESVETRRGTQEVQREMTD
                     TQAEKPIPVPVLEKKEAPLQEVIIPPEKPKIAETPKPPREPVQKKEPPLITPEPQGPV
                     RDAVINKLAANIRTRPDLNAPRFAMLFQGETVRIIDETTDEKGERWYKIIVLGRRIGW
                     VSESVVTLK"
     gene            complement(4172..6073)
                     /gene="selB"
                     /locus_tag="PK125_12025"
     CDS             complement(4172..6073)
                     /gene="selB"
                     /locus_tag="PK125_12025"
                     /inference="COORDINATES: protein motif:HMM:TIGR00475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HPB38871.1"
                     /translation="MIKRIVIGTAGHIDHGKTTLIKALTGIDCDRLKEEKERGITTEL
                     GFAHYKFGEDLLVGIVDVPGHEKFVRHMVAGAWGIDMALLIVAADEGVMPQTREHVDI
                     CELLGLKRGIVAITKKDLVDDDMVELVREDIEDFLKGRAFEGSPIIPVSSTTGENIDL
                     LKEEIRKTAENINERSKEGIFRLPVDRVFTIKGLGTIITGTCISGQIRIGEEIEIYPD
                     HRRARVKNIQAYHENVAEATAGQRVALNLQGIERNEIERGTIIGRPDTLMLSNRMDAS
                     FRYLKLPFKPIKTDSVLRFHIATTQEEVRLVLLTKDLIEPGEELFVQFVFAQPIVALP
                     DDRYILRGSYAIQTIGGGRVLDIMPRKHKRYADYLSSTCTILSDNNWAQKAEYHILKG
                     GYEGVNQNLLAMVLGKHEQFVAGIINSLISQGKVKLVGKIIIHMDRFKEYKKMTSTFL
                     ADFHKKNPLKIGVSKEELRTRLPKVDPSVFQAALDELMKDGFLEVDKDRVKIKSVARN
                     KDKDIEALEAQILKGLLRHGFTPPGIKDFAVEVRQSEGRLRDVLERLIFEGKAVKVKG
                     DLYFHSDVIEDLKKKVRSYLSEKKEMTPSEFKSVLDLSRKYMIPLLEYLDEIKVTIRT
                     GDKRILRSS"
BASE COUNT         1783 a         1344 c         1364 g         1665 t
ORIGIN      
        1 atgtctctga ggatggcaga gaaggattcc aggccttcat tgagaagaga aaaccagatt
       61 ttaagggaag atagtcacaa gtgatctttt ttggatcaac aaaatacaca taggaggtag
      121 ttacatgaag acttttacca aggcgtacat cccttacaac ggctattatt ctacaccctt
      181 tacccgctgg cagggtagta tgcagaatga aaacgcgatt gagttaggtg ccaaaacagc
      241 gcggagatgg ttcctggaaa aaaagaagat tgatcctacg gtcattgatt atttgtactt
      301 cgggatcagt attcatcagc accatctttt ctacagccac aactgggctg ccgggatgct
      361 aacagataac aagaagaatg tgcccggtct catggttaac caggcctgta caacctctac
      421 taccattttg agccttctgg caaaggatat cgagctgggc ggctatgatg tgggctttgg
      481 tcttatggca gaccgctgtt caaacggtgc ccatacagta tggccgaacc cgatgggtcc
      541 cggtggcgaa gttatccatg aaaactggat gatggacaac ttcaacagtg atcctaacgt
      601 aaaaccaccc acaaagatgg tgggaactgc agagaatgtg gcaagattgg aaggatttac
      661 caaggaacag tccgatgcag tggttcttcg ccgttatgag cagtaccaaa tggctctagc
      721 tgatgacagg gccttccaga aacgctatat gttccctgca gaggtgaaag caggcaaaaa
      781 agggaccaaa ctggttgagc ttgatgaggg tgccacccca accaccgccg aagggcttgc
      841 gaagttaaaa ccagcagagc caggtggcat ccacagtttt ggcgcccaga cattccctgc
      901 tgacggaaac gttggtttta tcgtcaccaa ccgtgacaaa gccaaggaat tgagtgctga
      961 tccaaaaata gagatccaga ttatttctta cggttattcc cgtgtcaatc caggctttat
     1021 ggctgcagca ccagtgccgg ctgcccagat ggcacttgcc aatgcaggtc tgaagatcac
     1081 agacatgaag gccattaaga gccacaaccc ctttaccacc aatgacctca actttgccaa
     1141 aaagatgggc attgatgtca tgttcatgaa caactacgga agctctctga tctacggaca
     1201 cccacaggcg ccaacagcag gcaggattat tgccgagatg ttagaagaga tggcgatgat
     1261 aggtggtggc tattgtctgt gggctgggtg tgctgcaggt gataccggtg catccatgat
     1321 ttttaaagta ggttgatcat tgttgaaaag ggatcgtatt ttcgcttccc ttagtccttt
     1381 agggctgttt cctccgcagg aaacagccct atttatactg tattttatga aaaacctttc
     1441 catttatatc caagagcagc atataaagta tatgggaagt gagtactctg tttcttgaaa
     1501 ttgatgctcg tatatgccaa agcactactc tcagattcag aaaaaaccat tttgccctta
     1561 ccaatgtaga aattgtattt caaaagggtt tttttgtcat taccaatcct tcctttggtc
     1621 ggaaggtaaa tgaagcaaac ggttcaacaa gaacctccag tacctgactg agaggctttt
     1681 tatattgaca agcaatatgg acgaagttat agcggctttc tctgatctgt gtgattatct
     1741 tatggaaact taagaaacta ctaaacccat aaccaatgaa aaaaacaaga aaaacaggag
     1801 taaatgaaac catgtctcaa taatcgcgtc atcgtggcag acaaaaaatt gactcctgtg
     1861 tatgacaata gttgtttatc atacatgaaa aatgttgtag aatctcgcta aatgaaccga
     1921 ttaatgacgg ccatactcct tgtgttggcg atagcatatg ttaatccatt atttggtaag
     1981 gaatatgttg tgaagaaggt tattgatggc gacacagtgc agctcgaatc tggcgagaca
     2041 gtcagatata taggtataaa tgcacctgag ctttatatga aggaaggagg ttcggaattt
     2101 tttgcaaaag aggcagcacg gttcaacaaa aaactcgttt ttatgaagaa ggttaggctt
     2161 gagtttgaca aagagaagca ggacagtcac ggcagaatgc ttgcctatgt ttttgtgaag
     2221 aacatttttg tgaacgcaga acttgtgaaa catggctatg caaaagtcat gataaaacca
     2281 ccaaacacga aatataagga tgtgcttctt gcccagcaac aaaaggcggt agaagaggtc
     2341 cggggtttat ggcaagaaaa aagacaagat acagaaacaa cttatatagg gaacaaaaga
     2401 acatatactc ttcacagacc atcttgtact tacgtaaaaa aaattcctga taaaagcaga
     2461 attaccttca gaagccgaag cgatgcaata aaaataggat atactccatg caggtactgt
     2521 aaaccatgag cgaacaatac attgaatact ggggtttaac ccagcaccct ttccttcttg
     2581 caccggatag taaaatgatg tgtgttactg gacaatattt cgaatgcctc gagcgtttga
     2641 aatacgccat atacacaaat aaggggggag tgctaattgt ttcagaggat gcaggactcg
     2701 ggaaaacaac aattctcctg aagcttatcg atgagatgaa agatgagtat ggcgacgcct
     2761 tcaggttcgc ctttgttgac cagccggcct taagtcctgc ccagatgatt gcccagataa
     2821 caggtagtat taccaaagcg gaacctcatg aagataagct aaaaaacctt aaaatgctaa
     2881 gagacgccct tcttgaagcg aatgagcatg gtggtaaaag catcattgtt gtagatgaag
     2941 cccaaatgct ctgtgaagca catgatgttt tgcaggagct cagaacactc atcaatctta
     3001 cccacaatgg tcaatacctc cataccttta ttctctcggg gcagcgggcc ctttggaata
     3061 cgatcaaagg aatgccggag ttctggcaga gactgcctgt tcgttactac tttatccccc
     3121 ttcgtgttga agaaacaaaa gagatgatca ggtttaggct cgcgaaggcc ggtctcgagg
     3181 agggacggga gatatttgat gatgattccc ttgagatcat ccatcgtttt tcgcgcggat
     3241 ctccaagaac agtcattgct ctgtcagatc ttgccctcct tgtgggctat acaaaccgtt
     3301 cagccaaaat aactttcaaa gaggtgtcaa aggcaatcaa tactatgtct ggcaaaggcg
     3361 agacgctacc ttttatgaaa acagaaaaac gtgaaggtat gccttcctta agtgccctct
     3421 caaatcttga caggggaaca gagattggtc atatacagga gggggcaggg gttggtggca
     3481 taaggggcag ggaaggctgg cagatgtggt tcatgcagta cggtcagtat gtgaggccac
     3541 ttatcgccct tcttgttatt accgctctga tcctcttgat tattatgggg tttcgttatg
     3601 tttttttcca aaagaaagaa agtgatgttg ccgaaatagc caagcaagag agtgtggaaa
     3661 caaggagagg tacacaagaa gttcaaagag aaatgacaga tacacaggca gagaagccaa
     3721 taccagtgcc agtactggaa aaaaaggaag cacctttgca ggaagtaata attccgcctg
     3781 aaaagcctaa aatagctgaa acccctaaac caccgcgcga acctgtgcaa aaaaaagaac
     3841 cgcctttgat aacaccagag ccccagggtc cggtaagaga tgctgtcata aacaagcttg
     3901 cagctaacat acggacccgc ccagatctca atgctccaag atttgcaatg ctctttcagg
     3961 gcgaaacggt tagaatcatt gatgagacga ctgacgaaaa aggggaaagg tggtataaga
     4021 tcattgtact tggccggaga ataggctggg tatcagaaag cgttgtaacc ctgaagtagt
     4081 tgaaggaatc tgtattgatc aagcaactga agggcacctg tcaatggtta tcagctcaat
     4141 ggccaccgag aactgagaac gattaattac attaactgct tcttagaatt cttttgtcgc
     4201 ctgtgcggat agtaaccttt atttcatcga ggtattccag aagaggtatc atgtatttac
     4261 gcgaaagatc aaggactgac ttgaattcag acggtgtcat ttcttttttt tctgaaaggt
     4321 aggatcgaac cttctttttt aaatcttcta tcacatcact gtgaaagtat aaatcccctt
     4381 ttacctttac tgccttaccc tcaaaaatga ggcgttccaa tacatcacga agtcttcctt
     4441 cggactgtct tacctctacc gcaaagtctt ttatgcctgg aggggtaaag ccatggcgaa
     4501 ggagtccttt cagtatctgt gcttccaggg cttcgatgtc cttatccttg ttgcgtgcaa
     4561 cagacttgat cttaactctg tccttatcaa cttcgagaaa gccatctttc atcaactcat
     4621 ccagggctgc ctggaacacc gatggatcta ctttaggcaa tctggttctc agttcctctt
     4681 tggatacgcc aattttcagg gggttttttt tatgaaaatc tgccagaaaa gtgctggtca
     4741 tttttttgta ctctttaaac ctgtccatat ggattatgat tttaccgacc aacttcacct
     4801 tgccctgact aatgagggag ttaatgatac cggcaacaaa ctgttcatgt tttcctaaca
     4861 ccatagcaag aagattttgg ttaacccctt cgtagccacc tttcaggata tgatattcgg
     4921 ctttttgtgc ccaattgtta tcagagagaa ttgtacaggt cgagctgaga taatcagcat
     4981 atcttttatg ctttcgcggc ataatatcga gcaccctgcc accgcctatg gtttggatag
     5041 catacgagcc tctgagaata tatctgtcat caggcagcgc aacgatgggc tgagcaaaaa
     5101 caaactgaac aaaaagctct tctcccggct caattaaatc ttttgtaagc aatacgagcc
     5161 tcacctcttc ctgggttgta gcaatgtgaa acctgagaac agaatctgtt ttaattggtt
     5221 tgaatggcag ttttaaatac ctgaaagacg catccatcct gtttgagagc ataagggtat
     5281 ctgggcggcc tataattgtt cctcgttcga tttcattcct ctcaatccct tggagattca
     5341 gggctactct ctgtcctgct gtagcctcag caacattctc atgataagct tgtatatttt
     5401 ttacccttgc ccttctatga tcaggataga tctcaatctc ctccccgata cgtatctgac
     5461 ccgatataca ggttcccgta attattgttc ccagcccttt gattgtaaag accctgtcta
     5521 caggaagcct gaatatacct tctttggaac gctcgtttat gttctctgct gtctttcgta
     5581 tttcctcttt taaaagatca atgttctcac ctgttgtgga agatacagga atgattggtg
     5641 aaccctcaaa agccctgcct ttgaggaaat cttctatatc ttccctcaca agctcaacca
     5701 tatcatcatc cacaaggtct ttcttggtga tggcaacaat gccccttttc aggccaagaa
     5761 gttcacatat gtcaacatgt tcccttgtct gaggcatgac accttcatct gccgctacga
     5821 taaggagggc catgtcaata ccccatgctc cggcaaccat gtgcctcaca aacttttcat
     5881 gtcctggaac atcaacaata cccaccaaca aatcttcacc gaatttgtaa tgggcaaatc
     5941 caagctctgt tgtaatgccc ctttcctttt cttctttcag cctgtcacag tcgatgcctg
     6001 tgagtgcctt tatgagggta gtcttcccgt ggtcaatatg gcctgctgtg ccgatgacga
     6061 ttcgctttat cattaattcc tactatacaa cattttgcag accttaacaa tgttctggcc
     6121 ctcattgccc gtcctgctat gtggagtctt ttgcgt
//
LOCUS       DAOOMC010000146         6093 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdus sp. isolate AMR_MDS_2685
            NODE_15421_length_6093_cov_6.265154, whole genome shotgun sequence.
ACCESSION   DAOOMC010000146 DAOOMC010000000
VERSION     DAOOMC010000146.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864760
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdus sp. (wastewater metagenome)
  ORGANISM  Syntrophorhabdus sp.
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae; Syntrophorhabdus.
REFERENCE   1  (bases 1 to 6093)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6093)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,729
            Genes (coding)                    :: 2,708
            CDSs (with protein)               :: 2,708
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 7 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6093
                     /organism="Syntrophorhabdus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15421_length_6093_cov_6.265154"
                     /isolate="AMR_MDS_2685"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2699756"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1010
                     /locus_tag="PK125_12030"
     CDS             <1..1010
                     /locus_tag="PK125_12030"
                     /inference="COORDINATES: protein motif:HMM:NF016424.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HPB38872.1"
                     /translation="GEVLGLRLLMSLDFGVQKHHCSIKKKSNRIFSAIPYSFLIFIFF
                     VALWPCSFRLFNFEIDFLSRAYATEIPPLKGYINDYANMIPPTIRTKLTNELREFERT
                     DSTQIIILTIPSLEGLTIEDYSIKVAEAWKIGKKGRDNGIILIIANQERKIRIEVGRG
                     LEGKLTDLMAGRIIDLVVKPRFKRGDFSGGLVAGIAALIDATKGEFKTDDDRPSRTIE
                     SFSPFFTILLFGAVGLLILGNISPVLGGVAGAIGLPVTAYFVLEPSVFTLILLCILGL
                     IMGIFLPFLFSGAGQGRGGMFFPRGGSFGSRGRGNDDFDSRGGFGGGGDFGGGGASGD
                     W"
     gene            1013..1669
                     /locus_tag="PK125_12035"
     CDS             1013..1669
                     /locus_tag="PK125_12035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB38873.1"
                     /translation="MYQAKADSFFTEDEKERIKVTTIGVEARTIGEIVTVVIDQSSDY
                     KEADVLGGVFIGGLASLLASSIFFNGSVWAFIPLAFILFFPGSMIFRWIPALKAVFIG
                     KKRKEKAVKERALRAFYERGLYKTKKHTGVLFFLSLLERKVWVLADKGIHDKIQPQTL
                     NKFAGMVSKGIREKRASDALCEAIIGVGELLWEYFPISDADTDELPNVVVLEQDVEKS
                     "
     gene            complement(1720..1941)
                     /locus_tag="PK125_12040"
     CDS             complement(1720..1941)
                     /locus_tag="PK125_12040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013821630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifU family protein"
                     /protein_id="HPB38874.1"
                     /translation="MKERVAAALEKVKPALKADGGDVELVDVTPDGVVKVVLKGACGG
                     CPMSQMTLKMGIERVVKQEVPEVKEVVAL"
     gene            complement(2077..2487)
                     /locus_tag="PK125_12045"
     CDS             complement(2077..2487)
                     /locus_tag="PK125_12045"
                     /inference="COORDINATES: protein motif:HMM:NF013920.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-ribbon domain-containing OB-fold protein"
                     /protein_id="HPB38875.1"
                     /translation="MGFEKFGRRSFTAMTKTAKFVDLLSEGKVAGTVCKQCGAKFFPP
                     RADCASCFSNDMDWFEMPGKGKLETFTIAYYAPFGFEKDCPYTMGVVDFGDMKLFARL
                     ASDIKPEDAKVGMDVSVKPLKYEDGQMSFEIIKA"
     gene            complement(2492..3673)
                     /locus_tag="PK125_12050"
     CDS             complement(2492..3673)
                     /locus_tag="PK125_12050"
                     /inference="COORDINATES: protein motif:HMM:NF012336.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HPB38876.1"
                     /translation="MAKDVAVIGVGQTYFVRGYEGSIRELAFEAFKEAMQDAGITQKD
                     VAASIFCSAPEYDKQRSPAGVLAEYLGLIPQPTFYIETVCSSSSSGVKVAYSMIKAGL
                     HDVVVVLGFQKMSEITSAESQERMGRGADIQWEAPFGTMMPAYYALYAQAYMAKNGLT
                     HDDLMNVRVKSGTYGQTNERAVYRKGVKPADFDPNNPDARMAGAVAWPLRVGDACANA
                     DGASCVILANAEKAKAFSKKPVWILGIGAASEAVNMAARPDFSKGLSVGYGAAAEAYK
                     MAGITAKDVKVAEVHDCFTIAEIMAYEGLGFAPMGEGKQLIREKATYKEGKIPVNVDG
                     GLLSKGHPIGATGGSQIRTIVLQLRDEAGPMQVPGSPEIGLVHNVGGVGLYGNVTIFG
                     R"
     gene            complement(3688..4692)
                     /gene="mltG"
                     /locus_tag="PK125_12055"
     CDS             complement(3688..4692)
                     /gene="mltG"
                     /locus_tag="PK125_12055"
                     /inference="COORDINATES: protein motif:HMM:TIGR00247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HPB38877.1"
                     /translation="MPKCVNRNIIVTIVFLFVITQTIIFAGIPQENNRAQINLVIKSG
                     TGLWNIAEKLKNDGVIYSSILFVLCTLPYRGRLIAGEYTLRKDMSIIDVVQRLGRGER
                     NIYALKIIEGYNLYNVAASIGKTAIMGGGDFLALASNKDFLQKIGIDADSLEGYLTPD
                     TYYYSKEVEADVFIEAIVKKTFRNLEKESIKKRMQELKFDIHKTLTLASIIEREAKLK
                     EEKPIISAVFHNRLQRGMSLDADPTVIYGTKAFNEPIKKTDLTTYTPYNTYAFAGLPK
                     GPICNPDWNSITAALYPASVDYLYFVSKNDGTHVFSRDMDQHNRFVTLYQRSKNAKKQ
                     "
     gene            complement(4673..5089)
                     /gene="ruvX"
                     /locus_tag="PK125_12060"
     CDS             complement(4673..5089)
                     /gene="ruvX"
                     /locus_tag="PK125_12060"
                     /inference="COORDINATES: protein motif:HMM:NF015607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HPB38878.1"
                     /translation="MRVLGLDVGNKKIGISLSDTTRSIAQGLKVYRRTTLEKDLEELK
                     LVVEEHGIGKIVIGLPKDLNGAIGKKAAEVMAFAEAVQKYTSIGIVLWDERFTTNEAN
                     RVFEMAQISHKKRKPYIDVMAAQIILQGYLDAQMRK"
     gene            5598..6041
                     /locus_tag="PK125_12065"
     CDS             5598..6041
                     /locus_tag="PK125_12065"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HPB38879.1"
                     /translation="MAQTEPVKLRVLTMKDLDAVTEIDYGLLGTKRRGYWEARLESLE
                     TSGVPSLIAEVDRKVIGFILGRASGWEYGIPENVAWIDTLGVIKEYQKRGVARILFKE
                     MFNMFKKVGVDTIYVFVNWKDWDLLQFFDKMGFKRGDMINLELKI"
BASE COUNT         1626 a         1364 c         1286 g         1817 t
ORIGIN      
        1 aaggtgaagt tctaggctta aggctgctta tgagtttgga tttcggggtt cagaaacacc
       61 actgcagtat caaaaagaaa agtaatagaa ttttcagtgc aattccatat tcgtttctca
      121 tttttatttt ttttgtagcc ttgtggcctt gctcttttcg tcttttcaac ttcgaaatag
      181 attttctttc cagggcctat gccactgaga tcccgcccct taagggttac atcaatgatt
      241 atgcaaacat gattcctccc actattcgaa caaagctgac aaatgagctc agggagtttg
      301 aacgaactga ctcaacacag attattattc taactatccc atccctcgaa gggttgacta
      361 tagaagacta ctcaattaag gttgcagagg catggaagat tggtaaaaaa ggaagagaca
      421 atgggatcat cctgattatt gcaaaccagg aaagaaagat acgcatcgag gttggcaggg
      481 gcttggaagg gaagttgaca gacctcatgg caggaaggat tattgatctt gtggtgaagc
      541 caagattcaa gaggggggat ttcagtggcg gtcttgtagc aggaatagca gcactgatcg
      601 atgcaacaaa aggagaattc aagacagacg atgatagacc ttccagaaca atagagtcat
      661 tttcaccctt ttttaccatt ttactctttg gtgcagtagg ccttttaata ttgggaaata
      721 tctctcctgt tcttggaggt gtcgcagggg cgattggtct ccctgttacc gcatactttg
      781 ttttggaacc ttctgtcttt acccttattc ttttgtgcat tcttggtcta atcatgggca
      841 tttttttacc ctttcttttt tcaggggctg gccagggcag aggcggcatg ttctttccca
      901 gaggagggtc ctttggttca cgcggtaggg gcaatgatga tttcgattca cgaggcggat
      961 ttggcggcgg cggagacttt ggcggcgggg gagcctcggg ggactggtaa cgatgtatca
     1021 ggcaaaagca gactcttttt ttactgaaga tgaaaaggaa aggataaaag tcactaccat
     1081 aggtgttgag gcgaggacca tcggagagat tgttacagtg gtgatcgatc aaagcagtga
     1141 ttacaaagag gcagatgttc tgggcggggt attcattgga ggccttgcat cacttcttgc
     1201 atcttcaata ttctttaatg gatctgtctg ggcatttata ccccttgcct ttatcctttt
     1261 tttcccaggc tccatgatct ttcggtggat tcccgcactg aaggcagttt ttataggcaa
     1321 aaaaagaaaa gaaaaagcgg taaaagaaag ggccctcaga gcattttatg aacggggact
     1381 ctataaaaca aaaaaacaca caggtgtact tttcttcctg tctttattgg aacgtaaggt
     1441 gtgggttctt gccgacaagg gaattcatga caaaatacaa ccgcagacat tgaacaaatt
     1501 tgcaggtatg gtttcaaaag gcataaggga aaaacgggcc tcagacgcct tatgtgaagc
     1561 aattattggt gtaggcgagc tcctctggga atatttccct ataagtgatg ctgatacaga
     1621 tgaactcccg aatgttgttg tgttagaaca ggatgtagag aaatcttaat ttttttcgcc
     1681 caagacagtc tcactacata gataattcta ttctgctgtt tagagcgcta caacctcttt
     1741 aacctcagga acttcctgct taaccactct ctcaattccc atcttcaggg tcatttggct
     1801 catagggcaa ccgccacagg cacccttcaa aacaactttt actacaccgt caggtgtcac
     1861 atcaaccaat tcaacatctc ctccatcagc tttaagcgca ggtttgactt tttctaatgc
     1921 tgctgccact ctttccttca tgtttcctcc ttactttctc atgtttgata caccaaacta
     1981 atgcagaaag gttcacctgt caacacaaat ttgctgcgcc aacagtgata aagaaagaag
     2041 actgccaact tgacagcctt ctttcttaac attttcctat gctttgatga tttcgaaaga
     2101 catttggccg tcttcgtact tcaggggttt gacggagacg tccatgccga cttttgcatc
     2161 ttcaggtttt atgtcgctcg caaggcgagc gaaaagcttc atatccccaa aatccacaac
     2221 tcccattgtg tagggacaat ctttttcaaa accaaaaggt gcgtagtaag caatggtgaa
     2281 ggtctcaagt ttgccttttc ctggcatctc gaaccagtcc atatcattag agaaacacga
     2341 agcacaatca gcccgtggtg gaaagaactt ggccccacat tgtttgcaaa cggtacctgc
     2401 aacctttccc tcagatagca aatcgacaaa ttttgcggtt tttgtcatag ctgtaaaact
     2461 tcttcttccg aatttttcga atcccataat atcacctccc gaagattgtc acgttgccat
     2521 acaagccaac gccaccaaca ttatgaacca gaccaatttc agggctgcct ggtacctgca
     2581 tagggcctgc ttcgtcacga agttgcagaa caatggtcct tatctgtgaa ccgcctgttg
     2641 caccgattgg atgtcctttg gaaagaaggc caccatcaac atttacaggg atcttccctt
     2701 ccttgtatgt agctttttcc ctaatgagct gtttcccttc acccataggg gcaaaaccaa
     2761 gaccctcata tgccataatc tcagcaatgg tgaaacagtc gtgaacctct gctactttca
     2821 catcttttgc agttatgcct gccatcttgt atgcttccgc agccgcgcca taaccaacac
     2881 tgaggccttt cgaaaaatca ggccttgctg ccatgttcac tgcttcagag gcagcaccta
     2941 taccgagaat ccagacaggt ttcttggaaa atgcttttgc tttctctgcg tttgcaagga
     3001 taacgcagga tgctccatcg gcatttgcac aggcatcacc tactctgagt ggccacgcta
     3061 ctgctccagc cattctcgca tcggggttgt ttggatcgaa gtctgcgggt tttacacctt
     3121 tcctgtaaac cgccctttcg ttagtctggc cataggttcc tgattttact ctcacattca
     3181 tgaggtcatc atgggttaac ccatttttcg ccatgtaagc ctgggcataa agggcataat
     3241 aagctggcat catggttcca aagggagcct cccactggat gtcagcgcca cgccccattc
     3301 tctcctggga ttcggctgag gtaatttctg acatcttctg gaagccaaga accacgacaa
     3361 catcatgtag tcctgcctta atcatgctgt atgctacctt taccccgcta ctgctggaag
     3421 aacagacggt ctcgatatag aacgttggct gtggaatgag acccagatac tctgcaagca
     3481 cacctgcagg cgatctctgt ttgtcatatt caggcgcaga acagaaaatg gatgcagcga
     3541 catctttctg cgttatgcct gcatcctgca tagcctcttt aaaggcctcg aaggccaact
     3601 ctctgatgga gccctcatag ccccttacaa agtaagtttg gcctacacca ataactgcaa
     3661 catcttttgc cataactcct ccttcgttta ttgttttttt gcgtttttgc tccgctgata
     3721 caatgttaca aatcggttat gttgatccat atcccgcgag aagacatggg taccgtcgtt
     3781 ttttgataca aaataaaggt aatccacaga agccggatac agtgcagctg ttatagaatt
     3841 ccagtcagga ttacatatag ggcccttggg aagccccgca aatgcatagg tgttatacgg
     3901 ggtataggtg gtaagatcag ttttctttat aggctcattg aatgcctttg ttccgtaaat
     3961 aactgtgggg tctgcatcaa gggacattcc cctttgtaaa cggttatgaa aaactgctga
     4021 tataattggt ttctcctctt tgagctttgc ctccctttca atgatcgagg caagggtaag
     4081 agttttatgg atgtcgaact tgagctcctg catccttttt tttatactct ctttttccaa
     4141 atttctgaag gtttttttca cgattgcctc aatgaaaaca tcagcctcta cttccttact
     4201 atagtaatag gtgtcagggg taagataacc ttcaagcgag tcggcgtcaa ttccaatctt
     4261 ttgaagaaaa tccttgttac tggccagggc aaggaaatct cctcccccca tgatcgcagt
     4321 ctttcctatt gaagcagcaa cgttgtaaag gttgtaccct tcaatgatct ttaaagcata
     4381 aatattacgt tctcctctgc ccaatctttg taccacgtct ataatagaca tatcttttcg
     4441 cagcgtatat tctcccgcaa taagcctgcc ccgataaggc agtgtgcaaa ggacaaagag
     4501 tatggaagaa taaatgacgc catcattctt caatttttcc gcaatattcc agaggcccgt
     4561 accacttttt ataactaaat taatttgcgc tctgttattc tcctgtggga tacctgcaaa
     4621 tataatcgtt tgtgttatca caaaaaggaa aactatggta acaataatgt ttctatttac
     4681 gcatttgggc atcaagatat ccctggagta ttatctgagc ggccatcacg tcaatataag
     4741 gttttctctt tttatggctt atctgggcca tctcaaaaac cctatttgcc tcattggtgg
     4801 taaaacgttc gtcccagaga acaataccaa tcgaagtata tttttgtact gcctctgcaa
     4861 aagccataac ttcagcagct tttttgccta tagctccgtt caaatctttt ggaagaccga
     4921 tgactatctt cccaatgcca tgttcctcaa caacgagttt taactcctcc aagtcttttt
     4981 ccaaggttgt tctgcgatag accttcagcc cttgcgcaat gcttcgggtt gtgtcagaca
     5041 aagatatacc tatcttcttg ttgcctacat cgagaccgag tacgcgcatg tgctattata
     5101 gcaattttca ttgaatattc aagcataata tggggtttct attgctgaga ctcatggtaa
     5161 cctgtccttg tatttctgct tgaggttgcc ctccaacttt atttattgta cagtgtgacc
     5221 ggctgattaa tagactcact gattactaca tacctactca aaatagaatc aaataaaaga
     5281 ttatctgtaa cataattgag tggattttgt aataatccgc ctgctcaact caatcgattg
     5341 tctgtcatcc cccccttcac ggtcgccctg cgagaaaagt tattccatag cccctaacca
     5401 attaatcgct taggggctag gtttccatcc ctcgagacac atgatatttg atacatatat
     5461 tcatttctct gatgcccatc gcttgcagtg aggaattcgt taacaccatc ctgttacata
     5521 agatttcctt ttcttgacaa cccctgtttc tttgctatac tcaaatgtta gaaaataaaa
     5581 catacaagag gattgtcatg gcccaaacgg aacctgtcaa gctacgagtg ttaacaatga
     5641 aagatttaga tgctgttact gaaattgact atggactgct tggaacgaag agaagggggt
     5701 actgggaagc aagacttgaa agtcttgaga catctggggt tccctctcta attgctgaag
     5761 ttgataggaa ggtcatcggt ttcattcttg gtagggctag tggctgggag tatggtatcc
     5821 ctgaaaatgt ggcttggata gatactctgg gcgtgataaa agaatatcag aaaagggggg
     5881 ttgcgcggat ccttttcaaa gaaatgttta atatgtttaa aaaggttggg gtagatacaa
     5941 tctatgtgtt tgtgaactgg aaggactggg atcttctgca attttttgac aaaatggggt
     6001 tcaaacgagg cgatatgatc aatctcgagc taaagattta aaagattcag tgcattacag
     6061 atggttccta gcacgggtaa aaagatatta ttt
//
LOCUS       DAOOMC010000147         6075 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdus sp. isolate AMR_MDS_2685
            NODE_15486_length_6075_cov_6.439369, whole genome shotgun sequence.
ACCESSION   DAOOMC010000147 DAOOMC010000000
VERSION     DAOOMC010000147.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864760
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdus sp. (wastewater metagenome)
  ORGANISM  Syntrophorhabdus sp.
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae; Syntrophorhabdus.
REFERENCE   1  (bases 1 to 6075)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6075)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,729
            Genes (coding)                    :: 2,708
            CDSs (with protein)               :: 2,708
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 7 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6075
                     /organism="Syntrophorhabdus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15486_length_6075_cov_6.439369"
                     /isolate="AMR_MDS_2685"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2699756"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..220)
                     /locus_tag="PK125_12070"
     CDS             complement(<1..220)
                     /locus_tag="PK125_12070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB38880.1"
                     /translation="MDKRKLERFKKSLLNMRGEILKKSKKLKEDSYTLGTDGIQDMAD
                     AASNSYNVDILMSISDNDIKLLKDIDNAL"
     gene            complement(213..668)
                     /gene="rsfS"
                     /locus_tag="PK125_12075"
     CDS             complement(213..668)
                     /gene="rsfS"
                     /locus_tag="PK125_12075"
                     /inference="COORDINATES: protein motif:HMM:TIGR00090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HPB38881.1"
                     /translation="MELTGKMRQAVKIEGEKYPAFWAREKLMDTFEKAQKCAQFADEK
                     KGRDIVILELIGLTDIADYFVLASATSERHVRTIAEAVEVGMKGIGVKPFSLEGYDDG
                     RWVIIDYQNVIIHIFIESLRELYDLESLWIEAKRYKLQKGNKLLGVDHG"
     gene            complement(708..1340)
                     /gene="nadD"
                     /locus_tag="PK125_12080"
     CDS             complement(708..1340)
                     /gene="nadD"
                     /locus_tag="PK125_12080"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: protein motif:HMM:NF000840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HPB38882.1"
                     /translation="MRVGIFGGTFDPVHNGHLRAAEEIGESFSLEKVYFVPAYIPPHK
                     NTGNISAMEDRLAMVRLAIKGNRRFSASDAELRRGGISYSIDTIEIMEKRFKDVYYLI
                     GVDAFDEIDTWHRYEDLFYHTNFIVMVRPNHRKKSGLTMFPKNVRKQMTPLNDYSFQH
                     NSGRQVYLQLITQLDISATRIRTSVGSRKSIRYLVPPAVETYIKEKGLYQ"
     gene            complement(1340..2584)
                     /locus_tag="PK125_12085"
     CDS             complement(1340..2584)
                     /locus_tag="PK125_12085"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: protein motif:HMM:NF001221.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HPB38883.1"
                     /translation="MKPDKLAMTAKKAAHTIAHTPTEQKDRALITLEQLIGDEKAYLM
                     LENKKDIETAEKAGLARSLVDRLRIDEKVIEEMQASIHDVVNLADPVGEIVKIWKRPN
                     GLQVGRMRIPIGVIFVIYESRPNVTIEAFSLCLKSGNCVILKGGSEASHSNRALFRLI
                     QEALKRTGITPEIVQLVDINDRSYIYDLLTLEEYIDLVIPRGGEELLRSVVEHSRIPV
                     LKHYKGVCHIFVDESADFPMAYEVCLNAKAQKPATCNAMETLLVHEAIAKDFLPEMIK
                     RLKKSGVKVKGCQRTIQIVNDIDPAQEEDWSQEYLDFVLSIRVVKDMDEAIDHIRTYG
                     SDHTEAIITKDYQRAWQFLKEVNSSLVLVNASTRLNDGFQLGLGAEMGISTTKLHAFG
                     PMGLEELTVTKFIAFGEGQLRV"
     gene            complement(2581..3684)
                     /gene="proB"
                     /locus_tag="PK125_12090"
     CDS             complement(2581..3684)
                     /gene="proB"
                     /locus_tag="PK125_12090"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR01027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HPB38884.1"
                     /translation="MLRAKRIVIKIGTSILLDRYKRVSIDMIRNFARQVKTLNDMKRE
                     VIIVSSGAIACGMETLNLKKKPKEIEKRQALASVGQVLLMKMYMDAFAWEKMRVGQVL
                     LTHEDIKSKDRCLNLMNTLNSLLSMDIIPIINENDALSFTEIKFGDNDNLSALIAQIT
                     DADLLLLLCDVDGLYDKDPTKHPDARLIRLIEKIDESVEKLAEGTKSEKSVGGMISKI
                     EAAKKAGYYGIPTRVIRGDIENIIVRVIKGEEPGTLFLPSKKISRRQWWAAFAYKTKG
                     TVWVDEGAEAAISNHGKSLLPSGITKTEGDFRRGECIEVNNNKGTVIARGIANYSSTD
                     VNKIKGLKSVDIAKKLGYKYTEEIIHRDNMVII"
     gene            complement(3677..4675)
                     /gene="obgE"
                     /locus_tag="PK125_12095"
     CDS             complement(3677..4675)
                     /gene="obgE"
                     /locus_tag="PK125_12095"
                     /inference="COORDINATES: protein motif:HMM:TIGR02729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HPB38885.1"
                     /translation="MKFVDEAKILVKAGDGGQGCTSFRREKFVPKGGPDGGDGGKGGD
                     VVISGSQNLASLLDFKYRRIFKAEKGKNGSSRNKRGRDGKNLIIHVPFGTVVFEGSSN
                     KLLVDITANQSTCIVARGGKGGKGNTHFVTPTHRVPYEFEPGEPGEEKELLLVLKLIA
                     DVGIIGLPNAGKSTLISRLTDATPKIGNYPFTTLSPILGVLREGDNAFVIADIPGIIE
                     GASQGRGLGLTFLRHIERTRMLLLVLDVSATNPVEDYNTLRHELNSYKRGMFSRQRIV
                     ILNKADKVSEEKADKWRSALIRKKEEVVVMSALKGLGIDALKEMIKMKDFNKVAHA"
     gene            complement(4672..4926)
                     /gene="rpmA"
                     /locus_tag="PK125_12100"
     CDS             complement(4672..4926)
                     /gene="rpmA"
                     /locus_tag="PK125_12100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HPB38886.1"
                     /translation="MAHKKAGGSSRNGRDSRGQRLGVKTYGGQTIKAGTIIMRQHGTK
                     IHPGKNVGIGRDNTLFALIDGTVKFDIAGDKKRAHVYPVE"
     gene            complement(4916..5245)
                     /gene="rplU"
                     /locus_tag="PK125_12105"
     CDS             complement(4916..5245)
                     /gene="rplU"
                     /locus_tag="PK125_12105"
                     /inference="COORDINATES: protein motif:HMM:TIGR00061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HPB38887.1"
                     /translation="MYAIIQSGGKQYRVMEGKKVKLEKLTVNAGDDVQIKEVLVFNDG
                     SNTVIGSPYVEGVSVNGKIISHGKNRKVVVFKYRRRKDSKTKKGHRQPYTEVLVEKIS
                     MEAPHGA"
     gene            complement(5452..5700)
                     /locus_tag="PK125_12110"
     CDS             complement(5452..5700)
                     /locus_tag="PK125_12110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB38888.1"
                     /translation="MPSRIVVNVEKMLDRGPEYGFLEAQINFEEKATPAKGMSFASVI
                     VSLAKTEVGGMTFDEIRAAALLKALSFLEACLKKPGTR"
     gene            complement(5841..>6075)
                     /locus_tag="PK125_12115"
     CDS             complement(5841..>6075)
                     /locus_tag="PK125_12115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB38889.1"
                     /translation="TIRCISSIDFNPPHSSDLSNKHSFCPLMEELNSNLTDLFQKTTD
                     TTIFYRTILKADSRQPFKRGQMLNKWYDSELNI"
BASE COUNT         1478 a         1463 c         1193 g         1941 t
ORIGIN      
        1 caagggcatt atctatatct ttgagaagct ttatatcatt atcgctgatg ctcatgagga
       61 tatctacgtt atatgaattg ctggccgcat cggccatatc ctggatacca tctgtgccga
      121 gcgtgtagga atcctctttc aattttttgg acttcttgag aatctccccc cgcatattaa
      181 ggagactctt tttaaaacgt tccaactttc tcttatccat gatccactcc taaaagctta
      241 tttccttttt gaagcttata tctttttgct tcaatccaca aactctcaag gtcatataat
      301 tctcttaacg attctataaa aatatggatt atgacgttct gatagtcgat tattacccat
      361 ctgccatcgt cataaccttc aagagaaaaa ggtttcaccc ctatcccctt catgccaacc
      421 tcaacagcct cagcaatagt tctcacatgt ctttcgctcg tggcacttgc caatacaaag
      481 taatcggcaa tgtcagtaag cccgataagt tcaagaatga cgatatccct gccttttttc
      541 tcatctgcaa actgtgcgca tttctgtgcc ttctcaaagg tatccatcag tttttccctg
      601 gcccaaaagg ctgggtattt ttccccttct atttttaccg cctgtctcat tttccctgtt
      661 aattccacgc ctcctgcttc tatttgccgg ttctattttt tcactattca ctgataaagc
      721 cctttttctt ttatatacgt ctccactgca ggagggacaa gatatctgat cgattttctg
      781 ctgcctacag aagtgcgaat tcgtgttgca gagatgtcga gctgcgtgat gagctgaagg
      841 taaacctgtc ggccagagtt atgctgaaat gagtaatcat tcaagggtgt catctgtttc
      901 cgtacgtttt tgggaaacat tgtaagcccg gacttttttc tgtgattcgg ccttaccata
      961 acgatgaaat ttgtatgata gaaaagatcc tcatacctgt gccacgtatc aatttcatcg
     1021 aaggcatcca caccaatgag ataataaaca tctttgaatc tcttttccat tatctcaatg
     1081 gtgtcgatgg agtaagagat gccgccacgt cttaactccg catcagaggc tgaaaatcgt
     1141 ctgttacctt ttattgccag ccgtaccatg gctaagcggt cttccatggc cgatatattg
     1201 cctgtatttt tatggggagg gatgtatgcc ggtacaaaat aaaccttttc aagagaaaaa
     1261 gactccccga tttcctcagc agctctcagg tgtccattgt gaactggatc aaaggtgcct
     1321 ccaaagatgc ctactctcat caaaccctca attggccttc accgaaagcg atgaattttg
     1381 taacggtcag ttcctccaga cccataggcc caaaagcgtg aagcttcgtt gtgcttattc
     1441 ccatttctgc tccaagaccc agttgaaaac catcattgag ccttgtggat gcgttaacaa
     1501 gcacgaggga ggagttcact tcttttagaa attgccatgc cctctggtaa tctttagtga
     1561 taatggcctc agtgtggtct gaaccgtatg tccttatatg gtcaattgct tcatccatat
     1621 ccttcactac ccgtatggag agaacgaagt caagatactc ttgggaccag tcttcttcct
     1681 gcgcaggatc tatatcgtta actatctgaa tggttctctg gcaacctttg accttaaccc
     1741 cgcttttctt tagccgtttt atcatttcag gcaggaaatc tttcgcaatt gcttcatgga
     1801 cgaggagggt ttccatggca ttgcatgtgg caggtttctg tgccttggca ttgaggcaga
     1861 cctcgtaagc catgggaaaa tcagccgatt catctacaaa aatatggcat acccctttgt
     1921 aatgcttcag aacaggaatg cgagaatgct cgacaacgct tctcaaaagc tcctccccgc
     1981 ctctcggtat cacgaggtcg atatattcct ccaaggtgag gagatcgtag atgtaggaac
     2041 gatcgtttat atcaaccagc tggacaatct ctggtgtgat accggtcctt ttaagagcct
     2101 cctgaattag cctgaaaagg gccctgtttg agtggctggc ctcagaacca cctttgagga
     2161 taacgcagtt tccacttttc aggcacagtg aaaatgcctc tattgtcacg tttggtcggg
     2221 actcatatat gacgaatatt actccgatgg ggattctcat gcgacccacc tgcagcccat
     2281 tcggtctttt ccatattttg acgatttctc ctaccgggtc tgccagattt acaacatcat
     2341 gaatacttgc ctgcatttcc tcaatgactt tttcatcgat tctcaaacgg tcaaccaggc
     2401 ttcttgcaag ccctgctttc tcggctgttt caatatcctt tttgttttcc agcatcagat
     2461 aggccttttc gtcgcctatg agttgttcca gcgtaatcag cgccctgtct ttctgttctg
     2521 ttggcgtatg ggctatggta tgggcagctt ttttagctgt catggcaagc ttgtcaggtt
     2581 tcatatgatc accatgttat ctctgtggat tatttcttca gtgtatttat agccaagctt
     2641 cttcgcaata tcaacgcttt tgaggccctt tatcttattt acatctgtgg atgaataatt
     2701 tgcgatacct ctggcaataa cagtaccttt attattgtta acctctatac attcaccccg
     2761 ccggaagtca ccttctgttt tcgttatacc ggaaggtaaa aggcttttgc catggttaga
     2821 gatggctgcc tctgcacctt cgtctaccca tactgtacct ttagtcttgt aggcaaaagc
     2881 tgcccaccat tgcctgcgag aaatcttctt acttgggagg aaaagggttc ctggttcttc
     2941 accttttatg acccgtacaa tgatgttttc aatgtcgccc cgtataaccc ttgtgggaat
     3001 gccgtaatag cctgcttttt tagcagcctc tatcttgctg atcatgccgc cgacgctctt
     3061 ctcgcttttc gttccttctg ccaatttttc gacgctttca tcaatctttt ctatgagcct
     3121 gatcaaccgc gcatcaggat gctttgtcgg atccttgtca tatagcccat caacatcgca
     3181 caggagcaga aggagatcag catccgtgat ctgggctatc aaggcggaaa ggttgtcatt
     3241 atccccgaac ttgatttccg taaatgataa ggcatcgttt tcattaatga ttggaattat
     3301 atccatggaa agaaggctgt tgagggtatt catcaaattg agacaccgat ctttgctctt
     3361 aatgtcctcg tgggtgagga gcacctggcc aacgcgcatt ttttcccatg cgaaggcatc
     3421 catgtacatt ttcatgagga gaacctggcc aacagaggca agagcctggc gtttttcaat
     3481 ctccttaggc tttttcttga gattcaatgt ctccataccg caggcaatgg ccccgcttga
     3541 tacgataatc acttcccttt tcatatcatt caaggtcttt acttgtctgg caaagtttct
     3601 gatcatgtca atgctgactc gcttatacct gtcaagcagt atggatgtgc caatcttgat
     3661 aacaattctc tttgctctaa gcatgggcaa ccttattaaa atctttcatc ttaatcatct
     3721 cttttaaagc gtctataccc aatcccttca aggcactcat caccacaact tcttcttttt
     3781 ttcggataag ggccgatcgc catttatcag ccttttcttc tgaaaccttg tctgctttat
     3841 ttaatattac aattctttgc cggctgaaca tccctctttt ataagagtta agctcgtgtc
     3901 tcaaggtatt gtagtcttcc accggattag tcgctgaaac atctaagaca aggagcagca
     3961 ttcgcgttct ttcgatgtgc cgtaaaaagg tcagtccaag gcctctcccc tgggaagctc
     4021 cttcaataat acctggaata tctgctataa caaaagcgtt gtctccttca cgcaggaccc
     4081 caaggattgg tgataaggta gtgaaggggt aattgcctat ctttggggtt gcatctgtga
     4141 ggcgtgaaat aagagtcgat tttcctgcat ttggtaaacc aataatgccg acgtctgcaa
     4201 tcagttttag cactaataac agttcttttt cctcaccagg ttcaccaggc tcgaattcat
     4261 agggcactct gtgggtcgga gttacaaaat gggtattacc ttttccccct ttgccccctc
     4321 ttgccactat gcatgtactc tgattggccg taatatcaac caggagtttg ttgctgcttc
     4381 cctcaaacac aacagtacca aagggtacat ggatgataag gtttttacca tccctacctc
     4441 ttttatttct actgcttccg tttttgcctt tttctgcttt aaaaattcgc cgatatttga
     4501 aatctaagag acttgcaagg ttctgtgatc ctgatatgac tacgtcccct cccttgccac
     4561 cgtcgccacc gtctggtcca cccttgggaa caaacttttc ccttctaaag cttgtacatc
     4621 cctggccgcc atctcctgct ttgacaagga tttttgcttc atcaacaaat ttcattcaac
     4681 agggtataca tgggccctct tcttatcgcc ggctatgtca aattttacag tgccgtcgat
     4741 gagcgcaaag agggtgttat cgcggccaat acctacattt tttcctggat gaatcttagt
     4801 tccatgctgt ctcatgatga ttgttccggc ctttattgtc tggccgccgt aggtttttac
     4861 cccgagcctc tggccacgtg aatcgcggcc atttctcgaa cttccgcctg cttttttatg
     4921 cgccatgggg tgcctccatt gatatctttt ctaccagcac ttctgtgtaa ggttgacgat
     4981 gacctttctt tgttttggag tcctttctcc ttctgtactt gaagacaacc acctttctgt
     5041 ttttaccatg ggagataatt tttccattca cagaaacccc ttcaacgtag ggacttccaa
     5101 tgacagtatt cgaaccgtcg ttgaagacga ggacctcttt tatctgaaca tcatcacctg
     5161 cattgacggt cagtttttcg agtttaacct tttttccttc cataaccctg tactgcttgc
     5221 cgccactctg tattattgca tacatgacct gactcctaac ctggttttaa aaaaactaca
     5281 tatagtatga taaagaactg gcaaaagtca ataaataact gaaattaagg gttttatgaa
     5341 ctttttgcag ccttattagc ctaaaaagcg ttcctaaccc tttttaggcg agtatggcaa
     5401 ggtgtaaaaa tttaatctgt cgggagttgc aggttatcag gaaaaaatcc atcagcgggt
     5461 gcccggcttt tttaagcaag cctccaggaa cgagagggcc ttcaataatg ccgctgccct
     5521 gatttcgtca aatgtcatac cccccacctc tgtctttgct aacgaaacaa ttacggacgc
     5581 aaaactcata ccctttgcag gtgttgcttt ttcctcaaag ttgatctgtg cctcaagaaa
     5641 tccatattcc gggccacggt caagcatttt ttctacattg actacaattc ttgatggcat
     5701 tgtatttctc ctgctgtttg aattttaaaa catattagca ctggttgtgg tgaaaaagcc
     5761 aatagaaatg attgtgttac aaagactcag gttcctcaca tattgtcttt tgacaaatga
     5821 aaagtcaggt gatatatatg tcaaatgttc aattcggaat cataccattt atttagcatc
     5881 tgccctcgct tgaagggctg cctggaatca gcctttaaaa tagtcctgta aaaaatggtt
     5941 gtgtcggtgg tcttctgaaa aagatcagtg agattgctat tcagttcttc catcaaagga
     6001 caaaatgaat gcttattgga caggtcggat gaatgtggag ggttaaagtc aatggatgag
     6061 atacaacgga ttgtc
//
LOCUS       DAOOMC010000148         6056 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdus sp. isolate AMR_MDS_2685
            NODE_15557_length_6056_cov_6.630728, whole genome shotgun sequence.
ACCESSION   DAOOMC010000148 DAOOMC010000000
VERSION     DAOOMC010000148.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864760
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdus sp. (wastewater metagenome)
  ORGANISM  Syntrophorhabdus sp.
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae; Syntrophorhabdus.
REFERENCE   1  (bases 1 to 6056)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6056)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,729
            Genes (coding)                    :: 2,708
            CDSs (with protein)               :: 2,708
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 7 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6056
                     /organism="Syntrophorhabdus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15557_length_6056_cov_6.630728"
                     /isolate="AMR_MDS_2685"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2699756"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            352..1176
                     /locus_tag="PK125_12120"
     CDS             352..1176
                     /locus_tag="PK125_12120"
                     /inference="COORDINATES: protein motif:HMM:NF024976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction enzyme HsdR N-terminal
                     domain-containing protein"
                     /protein_id="HPB38890.1"
                     /translation="MALNLDAEAVAAALRADVDLAPSGAKRLKLRTLLAKFGYTKRTD
                     SNTGEITRLLSEVGLSLNPPIIRFGVTWEITQDDWIILSNDVPNVPLPDSLPSTWNAD
                     GWFDRIVNLELRTEKEVEIKFIVPLLARLGYGDDDRYDGMPVPAAYGSRPTTLVIDFA
                     LFNSTESSLRNQPLLTVEAKREGRLVKQRELESAHNQAKSYCLWAQCDYFMITDSRTI
                     QAFHIARGQLGELAPLFVCERNDLTNKFGELYALVSKKTLTQYYLAKFASADEALS"
     gene            1244..1669
                     /gene="umuD"
                     /locus_tag="PK125_12125"
     CDS             1244..1669
                     /gene="umuD"
                     /locus_tag="PK125_12125"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF012920.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V
                     autoproteolytic subunit"
                     /protein_id="HPB38891.1"
                     /translation="MKTPLFASPISAGPPSPADSTIDQSIDLNEFLIKNPTATFFVRV
                     EGRSMVNAGIHPGDILIVDKSIDPLPNNIVIAVVDGEFLVKRLITECGERVLVGDSPD
                     YGRIELRNGSQVELWGVVTWVIHKIREEKHLSMTRSCKG"
     gene            complement(2334..2588)
                     /locus_tag="PK125_12130"
     CDS             complement(2334..2588)
                     /locus_tag="PK125_12130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB38892.1"
                     /translation="MPTLSDIYQLCWVIRDFAVKTSDRNIIRYQTDQIIQVPKNEIDE
                     LEKNGFVIPIFLNMPYFYVTYGDLLSRSRDKLNGEIIASR"
     gene            complement(2572..3294)
                     /locus_tag="PK125_12135"
     CDS             complement(2572..3294)
                     /locus_tag="PK125_12135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB38893.1"
                     /translation="MRLEEINYNSINLKCGYVTYMLLRKPSGFQVYSRENHCMSWDCE
                     ECRPRLNRQWKLAIEKAFNERNGPHIFVGHKSSTQKELVEFIKENIDRGTSYLKIYVR
                     GGAYLIATRSFPRSRRRHKDKFIKVDLDKILNEPWEKIGREHRISSSRGFVTLPKKEP
                     KNLCVATLNRHQFHEIDGTNLNEDEGIEYLRNKVDRKNLTKLGRKIIQDIYPEKPSGA
                     NLIKEQKGINGINAGGLYANPQ"
     gene            complement(3560..3787)
                     /locus_tag="PK125_12140"
     CDS             complement(3560..3787)
                     /locus_tag="PK125_12140"
                     /inference="COORDINATES: protein motif:HMM:NF024139.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HPB38894.1"
                     /translation="MAADNLSVNQDDRLINEKDVQSLLGIGLSTIQQWRLKGQGPKFV
                     KLGRLVRYRHKDVLEYINKLKSYSSTSDDAK"
     gene            complement(3897..4556)
                     /locus_tag="PK125_12145"
     CDS             complement(3897..4556)
                     /locus_tag="PK125_12145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB38895.1"
                     /translation="MRLNEHDRMMAIARHPEYIKDYNEIKDLFYKCKRDKSNGSCVDT
                     AIDRLRIKWNISTPILIPTQEDRQILFHQDEQDAIVELTDSEYDLRRPANALGINPEF
                     PTRQYILLVHLEINLTKSEADLIRSFKSMIKSWKKNIPDDIAPRKSKYDPWKIYDLHQ
                     SNRSLNQIARDLCGDNSPRGERSPAYNDRLWPPYKAVERAYKKAVKMIQSVTYSSQKF
                     K"
     gene            complement(4642..5859)
                     /locus_tag="PK125_12150"
     CDS             complement(4642..5859)
                     /locus_tag="PK125_12150"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase family protein"
                     /protein_id="HPB38896.1"
                     /translation="MNKIRLIKSEIDKLPKPKEGERAEYFDKKLPGFGLRVTKKKKVY
                     FVLGRVKGKLVRYTIGKHGLITPDNARDIAMEKLVEMSNGINPNKKKAQDRVRGITLK
                     LAMDEYFVSRPALRDSTKKGYKCIIGKWLPDWMDKPLDEINKSDVAKRHLRIAKDRST
                     VMANNVMRTFRIFYNHAREVSDGALPETPTKRLSQSRQWFPVHRRQTVIRESELNKWL
                     DAVKEFSNPVAQDALLLLLLSGCREQEILSLLWDDIDMRGRTFTIRADISKNHREHSL
                     PMSKAILDIFNRRLALRENDWVFPSHGPKGHLTELKRAVEFVIDKSKVQFCIHDLRRT
                     FTGIAEQEVSYTMLKRLLNHYTGNDVTQGYLVIPMDQLRDPMMRITNRIQKAFKAKDK
                     RGARADKVVPLRA"
BASE COUNT         1607 a         1314 c         1279 g         1856 t
ORIGIN      
        1 ccacagggtg ttcagtgcga gctccaggta catggtggat tccgtcggga gcttcttctc
       61 gatgcgacag tcgtcgattc cctgcaaagt atcttcaaaa cacgcctgct ttgtctccca
      121 cccagaagag attgtatctg aaaccaaaga gaatccaact ggtgcgcgac atcagtttgt
      181 cgcataattt tatgacctcg atggtactcc ctgcaatgtg gatgtcagtg gcaattagca
      241 ggttgttatg taccaagcat ttggatatac ttactttgtt ttctgatttg agatgtgaag
      301 ttgccttcaa aggaaagcac agattagaca gcatgctgtt ggagtcacat aatggcgctc
      361 aatcttgatg cagaggctgt tgcagcagcg ttaagggcgg atgttgacct ggccccgtct
      421 ggcgcgaagc ggctcaaact ccgtactctg ctcgccaagt tcgggtacac caagcgtact
      481 gacagtaata caggtgagat cacccgcctt ctcagcgagg tgggtctgag cctcaaccca
      541 ccgatcattc gtttcggagt gacatgggaa ataacccaag acgattggat cattctttcc
      601 aatgacgttc caaacgtgcc actgcccgac tctctgccat cgacatggaa cgctgatgga
      661 tggtttgacc gtatcgtaaa tctcgagttg aggaccgaga aagaggtcga gattaaattc
      721 attgtacctc tactcgcacg tcttggttat ggggacgatg acaggtacga tggaatgccc
      781 gtgcctgcag cctatggatc aagaccaaca acgctcgtca ttgactttgc cctgttcaac
      841 agcacggaat cgtcgttacg caatcagcca ctgctcaccg tagaggcaaa acgagagggt
      901 cggcttgtta aacaacgtga gcttgagagt gcacacaatc aggcgaagag ctattgtctc
      961 tgggctcaat gtgattactt tatgatcact gacagccgta ctattcaagc cttccatatc
     1021 gcacgtggac aattgggtga acttgcaccg ctctttgtgt gcgagcgcaa tgatctaact
     1081 aacaaattcg gtgaactcta tgccctggtg tcgaaaaaaa cactgactca atattacctt
     1141 gccaagttcg cttctgcgga cgaagctctc tcataatgct gaacgaagaa ctatggaaga
     1201 gctcaagacc ataacgtcat ggtatcacga caggaagccg tttttgaaga cccctctctt
     1261 cgcctcccca atctccgcag gtccaccatc gcctgcagac agcaccatag accaatccat
     1321 agacctcaac gagtttctca tcaagaaccc gaccgccacg ttctttgtga gggttgaagg
     1381 gagatccatg gtcaatgcag ggatacatcc cggtgacatc ctgatcgtgg acaagtccat
     1441 cgatccgctg cccaacaaca tcgtgatcgc cgtggttgat ggagaatttc ttgtgaagcg
     1501 gctcatcacc gagtgcgggg agcgggtatt ggtcggtgac agccctgatt atgggaggat
     1561 tgaattgcgg aatgggagtc aggtcgagct ttggggagta gtcacatggg taatacacaa
     1621 gatcagagaa gagaaacatt tatcgatgac caggagctgt aaagggtaga gaaatagtat
     1681 gaaagtatta tcatttatgt atctgggata aacctaggag gattagcatt cagtgatatc
     1741 tcttgaagag gttctggaag aggttcagaa aaacaatcgt gtatgtcccc aaccgaagag
     1801 ctggcaacag ctttatgata tactacctca taagcagcac gcagatggag ggtgagaacc
     1861 atttcttccg ttgattttag tagcatggtg ggacactcct aatctttcca agatgctccg
     1921 ttttcatgag cacattaagt gggcagcaga ccatggttgc cttgaggaag tctttatgtt
     1981 cttgcaaaat ataccagaag atcagtggca ccattttgga gagtagtaca gctcatctcc
     2041 tgtatgactt cattttgggt ttccagaccc agacagagat gatttactaa tgattcccta
     2101 atgcagatct catctatcat tggcagaatc ttgattcatt actggaatgg gggtttgctg
     2161 caaacccttt gggtttgata ggattgcggt atatatataa atatatatac tctgcatcgt
     2221 ggttgacctg cctgaaaaat ggacttggac ctgagggccc tggagtccca atcagtgcta
     2281 gccccacccc ctctgggccc tgccaccccc tccttgccat atcgaggcag atattaacgt
     2341 gaggctataa tctcaccatt taatttgtca cgagatctcg atagtagatc cccataggtc
     2401 acatagaaat atggcatatt gaggaagatc ggtattacaa aaccattttt ctcaagttca
     2461 tcaatttcat ttttagggac ctgaattatc tgatcggttt gatatctgat aatatttcga
     2521 tctgaagttt taactgcaaa gtctctaatt acccagcaga gttgatatat gtcactgagg
     2581 gttggcatat aatcctcccg catttatgcc attgattcct ttctgttctt taatgagatt
     2641 tgctccactt ggtttttctg ggtaaatatc ttgtattatc ttcctaccaa gcttagtcag
     2701 gtttttccta tccactttat tcctcaagta ttctatccct tcatcttcat tcaagttggt
     2761 tccgtcaatc tcatgaaact gatgacgatt aagggtagca acgcataaat ttttgggctc
     2821 tttctttgga agtgtaacaa aacccctgga tgaagatatt cggtgctctc ttccaatttt
     2881 ttcccatggc tcattaagga ttttgtcaag gtctactttg ataaatttat ctttgtgcct
     2941 tctacgggat cttgggaatg accttgtggc aataagatat gctcctcctc tcacatagat
     3001 ttttaaataa gaagtgcctc gatcgatatt ttctttaata aattctacca attctttttg
     3061 tgtggacgat ttatgcccaa caaaaatatg tggtccattc ctctcattga aggctttttc
     3121 tatagccaat ttccattgtc tgtttaatct aggtctacat tcctcgcaat cccagctcat
     3181 acagtgattt tctcgagaat atacttgaaa tcctgagggc tttctcaata acatgtaagt
     3241 aacatatccg cacttcaagt tgattgaatt atagttgatt tcttcaagtc tcatgttagt
     3301 tatcaaccta tatgaccaac aaaattagta agttataaga aacaatcata acttattaat
     3361 aatgttgatg aaaataatga tcccattggg ttaaaatact tatactctcc atcagtgttt
     3421 aacattagga ttttagtaat attctggttc gcgcaactta ctaaaattat aagagatatt
     3481 atggcggcaa ggtaacttac taatattgtt agagataatg attttttcat atgaatattt
     3541 gctatagata tcaaggtgct catttcgcat catcgcttgt actagagtaa gattttaatt
     3601 tgttaatata ttccagcaca tccttatgac ggtatcttac caggcgacca agttttacaa
     3661 attttggccc ttgccccttc aaacgccact gctggattgt gctcagtcca attcctaaaa
     3721 gactttggac atctttctca tttatgagcc tgtcatcttg atttactgag agattatcag
     3781 cagccatacc aaacctccta gtattgtagg ttggtatggg tatagcgata agaatttgtt
     3841 ttgaatattg acgttaagag tattcgctta acgtctacca aatggattga aatagttcat
     3901 ttgaatttct gacttgagta ggtaactgat tgtatcattt taactgcctt cttataggct
     3961 ctctcgacag ctttataagg aggccagagc ctgtcattat atgcagggct tctttcgcca
     4021 cggggagaat tatctccaca aagatctcgg gcgatctgat ttaatgatct attagattga
     4081 tgcaggtcgt agatcttcca tgggtcatat ttactctttc tcggcgcaat gtcatcgggt
     4141 atattttttt tccatgactt gatcatactc ttaaaggacc ttatgagatc agcctcgctc
     4201 ttagtcagat tgatttctaa atgtactagg agaatatatt gtctagtggg aaattcaggg
     4261 tttatgccaa gggcatttgc tggcctacgc aaatcatact cagaatcagt caactcaacg
     4321 atagcatctt gttcatcttg atgaaataag atttgtctat cctcttgggt gggtatcagg
     4381 ataggggtgg agatgttcca cttaatacgt aatctatcta tagctgtatc cacacaactt
     4441 ccattcgatt tatctctttt gcatttataa aagagatcct taatctcatt ataatctttt
     4501 atgtattcag ggtgccttgc gatcgccatc attctatcgt gctcattcag tctcacattg
     4561 aatctcctcg gttagtaaga aatggcgata aactttaaac cgttaggacc ttgcaaccaa
     4621 ggtttcccat ctcagaaaac attatgccct caatgggaca accttgtcag ctcttgcacc
     4681 tctcttatct ttagccttga aagccttctg gatcctgttt gtgatcctca tcatgggatc
     4741 acgaagttga tccatgggaa taacgagata accttgggtg acatcgttgc cggtataatg
     4801 gttcaggagg cgtttcagca ttgtgtagct gacctcctgt tcagcaatgc ctgtaaatgt
     4861 tcttcttagg tcatggatgc agaactggac cttagactta tcaataacaa actcaactgc
     4921 tctcttgagc tctgtgagat gacctttggg tccatggctt gggaataccc aatcattctc
     4981 tctcaaggcc aatctacggt taaatatatc gagtatagcc ttggacattg ggagagaatg
     5041 ttcacggtga ttcttagaga tatccgcacg aatggtgaat gtcctgcctc tcatatcaat
     5101 atcatcccat agtagggaga ggatttcttg ttctcgacat cctgagagaa gaagtaggag
     5161 cagggcgtct tgagcaacag gatttgagaa ctctttaact gcatcaagcc atttattcag
     5221 ttcgctctca cggatgacag tctggcgacg gtgtacagga aaccattggc ggctttggga
     5281 gagtctttta gtgggtgtct ctggaagagc cccatcagac acttcccgag catggttata
     5341 aaagatccgg aatgttcgca tgacgttgtt agccataacg gtactccgat cctttgctat
     5401 cctgaggtgt cttttcgcca catcgctctt gttgatctca tcgagtggtt tgtccatcca
     5461 gtcaggtagc catttcccaa taatgcattt gtaacccttc ttggtgctat ctctaagggc
     5521 aggtcgagaa acgaagtact catccatggc cagttttagt gtaattcccc ttacacggtc
     5581 ctgggctttc ttcttgttag gattgatacc gttggacatc tctacaagct tctccatagc
     5641 tatgtcccgt gcattgtcag gggttataag accatgtttt ccaatagtat aacgtaccag
     5701 ttttcctttc actcttccga gcacaaaata aacttttttc ttttttgtta ccctcaaccc
     5761 aaacccgggg agctttttgt caaagtattc agctcgctct ccctcttttg gttttggtaa
     5821 tttgtctatc tcagacttta ttaagcggat cttgttcata agtagcaacc tcaaaaatgg
     5881 gtaacatatg ggtaacaaaa aacccgaaaa gtgtcaatac tcgatactag atgatgttat
     5941 taactatatg attataaaag gtatataagt ttatccaaaa ttgcacacaa taacgacgat
     6001 aagactacga atccaaaggt cgcaagttcg agtcttgctg ggcgcacttt tccttg
//
LOCUS       DAOOMC010000149         6035 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdus sp. isolate AMR_MDS_2685
            NODE_15632_length_6035_cov_5.745987, whole genome shotgun sequence.
ACCESSION   DAOOMC010000149 DAOOMC010000000
VERSION     DAOOMC010000149.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864760
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdus sp. (wastewater metagenome)
  ORGANISM  Syntrophorhabdus sp.
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae; Syntrophorhabdus.
REFERENCE   1  (bases 1 to 6035)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6035)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,729
            Genes (coding)                    :: 2,708
            CDSs (with protein)               :: 2,708
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 7 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6035
                     /organism="Syntrophorhabdus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15632_length_6035_cov_5.745987"
                     /isolate="AMR_MDS_2685"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2699756"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1016
                     /locus_tag="PK125_12155"
     CDS             <1..1016
                     /locus_tag="PK125_12155"
                     /inference="COORDINATES: protein motif:HMM:NF017200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HPB38897.1"
                     /translation="NLLPFDRSGVAAIIESSSRFADHQDKLSAKFSTIMDLLRESHYW
                     AKKAESNLVKGEHVKKALDEKILRINRVEERMREMTLEDTIIVNTAGTKVGQVNGLAV
                     LDFGDYSFGKPSRITAKTFTGKAGIVNIERETKMSGKIHEKAILIISSYLGSKYAIKN
                     PISLSASITFEQLYEMIEGDSATCAELYALLSSISGVPLKQSFAVTGSMDQNGDVQPI
                     GGVNQKIEGFFELCKMRGLDGSHGVIIPGRNMKHLVLREEVIDAVREGKFSIYTIDRM
                     EEGLEILTGMSAGELDENGTYPEGTINYLVAKRLTEIGEALEKKKEKEGNNILAKAPG
                     EDQ"
     gene            1134..1691
                     /locus_tag="PK125_12160"
     CDS             1134..1691
                     /locus_tag="PK125_12160"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005777 - peroxisome [Evidence IEA];
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003986 - acetyl-CoA hydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0008413 - 8-oxo-7,8-dihydroguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_function: GO:0010945 - CoA pyrophosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035539 - 8-oxo-7,8-dihydrodeoxyguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0009132 - nucleoside diphosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0015938 - coenzyme A catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HPB38898.1"
                     /translation="MISYIRECLTTYKPENIHCVRSKWAGVVIPIHGMGNELFIILTK
                     RTQTVRNHKGEVSFPGGMFEEKDGNRMVTAVRECCEEIGVKEKDVEILGRIDDMYTMT
                     GFCVRPYVASIPFPYKFKPNPEEVAYIISLPFETLRNVSPGLEQAELNGHVEKVPSFY
                     YRGDRIWGATCRILLRLRWILHGKV"
     gene            1678..2838
                     /locus_tag="PK125_12165"
     CDS             1678..2838
                     /locus_tag="PK125_12165"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPB38899.1"
                     /translation="MGRFNLKVLIILSLGHMVTDIYQGALPATLPFLKDKLGLTYTMT
                     GVILMVANFTSSILQPLFGFYSDKKEKAALLPIGLLAAGIGYALLALPADYYMVLFLV
                     IISGLGIAAYHPEGYKTAHFFTGKRSVTGMSVFSVGGNLGLAIGPPMALYVTRYLGFD
                     ALPVAVIPSLLCTALMLTQRKTIAIPRLEHNIKQAETSKTTASSYVSLFLVVAIVIMR
                     SWTQMGLLSYIPFYYINHLKGDPLFAGNLIFVFLACGAAGTLIGAPFADRWGHRFFIR
                     LTMFLATLILPLMFVPFIAKSYLLFVVLGLEGLILISTFSVTVVMAQRLLPHRLGIAS
                     GLMVGFAIGTGGIGVTILGVIADNFGVPVALEAIMVLPFIGFLLSLVVKYKE"
     gene            2835..4226
                     /gene="der"
                     /locus_tag="PK125_12170"
     CDS             2835..4226
                     /gene="der"
                     /locus_tag="PK125_12170"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HPB38900.1"
                     /translation="MSQKPIVTIVGRPNVGKSTLFNRLIGYRKAITEDTPGVTRDRNY
                     GEFEYAGTDFRLVDTGGFEPTKEEGYFPLMRQQITISLEESSMIIFLLDGKEGLLPQD
                     MEIAKGLRRYGKPIFFTVNKVDSGKRELLTPEFYTLGTEKLYPVSSLHGLGIDELLED
                     LVSTAKQVMNDKDPQKTRKRRKHTDDVIEKDSISEAMDMELSTEIKIAFAGRPNTGKS
                     SIANRLLGSERMIVSNLPGTTRDAIDSQVAFRNRNLILIDTAGLRRKSRIEAKVEEHS
                     VVSALRTIERADVVNLIIDANEGVSHQDGGIAHTIITRGKGLCIVVNKWDLVENKISM
                     EEYRKMVHERIPHTSFAPVICVSAKTGKNIKKILESDLRIYSQLNRRIKTSALNKTLE
                     EIIQAQSLPYQHGKRVNIIYMNQVKVMPPTFALFTNFPRLIPEHYKRYLENSLRQKYG
                     FTGVPIRLLLRKK"
     gene            4279..4659
                     /locus_tag="PK125_12175"
     CDS             4279..4659
                     /locus_tag="PK125_12175"
                     /inference="COORDINATES: protein motif:HMM:NF016763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HPB38901.1"
                     /translation="MSLKIRVKRFFYYWFPILCFMALIFFLSSLHGSSISHFGFLDVL
                     VKKGAHITGYAVLYFLLFRAFQSLMTPRRALFVSAVIAVIYAISDEYHQTFVPFREGT
                     IRDVFIDSIGVFLVYLFLRQRYNH"
     gene            4797..5198
                     /locus_tag="PK125_12180"
     CDS             4797..5198
                     /locus_tag="PK125_12180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB38902.1"
                     /translation="MRIVENEKKPAVSVEELLDLLQAKKEKEARHPERQKSFFQSIPV
                     ATIILGLLIVALGAMVIILKSDIASLKNDITDLKNLKTQVAALDPKLQITTLEGKLED
                     FKKEREVIKGEVTQLRGEMEALKTDRKKGKK"
     gene            complement(5259..5807)
                     /locus_tag="PK125_12185"
     CDS             complement(5259..5807)
                     /locus_tag="PK125_12185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB38903.1"
                     /translation="MKLLFTRLQEEVITVKRSVTRVQVETGASRIKPYKFQVIDITPE
                     PCEKKREDAQGVSKLSSEDQKNSLSLNVKSEYVSDIVDLQGTPDKGNSLTPVIYSAGS
                     ILKGNHSREKSVDKSFLSSKTGYIQKNEPSFNKSADSVDFSMVDESLARYRFTTRKFI
                     STQEMIEYHRAQEKRVSFELTI"
BASE COUNT         1686 a         1329 c         1374 g         1646 t
ORIGIN      
        1 aaaatctcct gccctttgac aggtctggtg ttgctgcaat tatagagtcc agttcaaggt
       61 ttgccgacca ccaggacaaa ctgtcagcga agttcagtac catcatggac ctcctgaggg
      121 agtcccacta ctgggcgaag aaggcagaga gcaaccttgt aaaaggcgag catgtcaaaa
      181 aggctttgga cgaaaagatc ctccggatca atcgtgttga ggaacgaatg agggagatga
      241 ctctggaaga taccatcatt gtaaacacag caggtacaaa agtgggccag gtgaacgggc
      301 ttgctgttct tgatttcgga gactacagct ttggaaaacc atcgagaatc actgcaaaaa
      361 cctttaccgg caaagctggc atcgtgaaca tcgagcggga aaccaagatg agcggaaaga
      421 tccacgaaaa agcgattctc attatctcga gttaccttgg aagcaagtac gccattaaga
      481 accccatcag tctgtcagca tcaatcacat ttgagcagct ctacgaaatg atcgagggtg
      541 acagcgcaac gtgtgcagaa ttgtatgcgc tcctgagcag tatttcgggc gtacctctca
      601 aacaaagttt tgcagtaact ggctccatgg atcagaatgg cgatgtccaa cccattggag
      661 gcgtcaacca gaagattgag gggttttttg agctctgcaa aatgcggggc cttgatggaa
      721 gccatggcgt gataatcccg ggaagaaaca tgaagcatct agtactcagg gaggaagtta
      781 ttgatgcggt tcgagagggt aaattctcta tctatacgat tgaccgtatg gaagaaggcc
      841 ttgaaatcct tacgggcatg tcagctggag aattagatga aaacggtaca tatccggaag
      901 gtacgatcaa ctatctcgtt gccaaaagac tgactgagat tggcgaagcc ctggagaaaa
      961 agaaagagaa agaagggaac aacattctag caaaagcacc gggggaagac caatgaacag
     1021 ttatcggtag ccggtaatca gtgaaaaagg cgttgtgctt ccagctctgt caacttagag
     1081 ctatgagctg atttaccatt gctttcataa gccttctgct gctaaaatac ctgatgattt
     1141 cttacatacg agaatgtctt accacatata agcccgaaaa cattcattgc gtcaggtcaa
     1201 aatgggctgg tgtagtcatc cccatccatg gaatgggcaa tgagcttttt attatcctta
     1261 ccaagaggac acagaccgta aggaaccaca aaggcgaagt ttctttcccc ggtggtatgt
     1321 tcgaggaaaa ggatgggaac cgaatggtta ctgcagtgag ggagtgctgc gaagaaattg
     1381 gtgtaaaaga aaaagacgtg gaaattcttg gcaggattga tgatatgtac accatgacag
     1441 gattttgtgt aagaccttat gttgcgtcaa taccttttcc ctataagttc aaaccaaatc
     1501 cggaagaggt ggcatacatt atctctctgc cgtttgagac tcttcgtaac gtttcacccg
     1561 gtcttgaaca agcagagttg aatggccacg tggaaaaagt tccgtctttc tactacagag
     1621 gtgatagaat ctggggcgca acgtgcagga tacttttaag actgaggtgg atattacatg
     1681 ggaaggttta atctaaaggt cctcatcata ctgtctcttg gtcacatggt aaccgacatc
     1741 tatcaggggg ctctgccagc cacccttcct ttcctgaagg ataagctcgg gctaacgtat
     1801 acaatgaccg gggtaatcct catggttgca aactttacct catccatact ccagcccctc
     1861 tttggttttt attccgacaa gaaagaaaaa gcagccttgc ttcccattgg actcctggca
     1921 gcaggcatag gatatgctct ccttgcacta ccggctgatt attatatggt gttatttctg
     1981 gtcattatca gcggactcgg catagcagcc taccatccag aaggatacaa gacagcacat
     2041 tttttcacag gaaagcgaag tgtgaccggt atgtctgtat tctcagtggg tggaaatctt
     2101 ggccttgcta tcgggcctcc catggcactt tatgtgaccc gctatctggg atttgatgcc
     2161 cttccggtgg ctgtaatacc ctccttgctg tgtactgccc tcatgctcac ccagagaaag
     2221 acaattgcca tacccaggct cgagcacaat ataaaacagg cagaaacatc aaaaacaacg
     2281 gcaagctcct atgtgtcact gtttctcgtg gtggccatcg ttatcatgcg gtcatggaca
     2341 cagatggggc ttctctcata tatcccgttc tattacatta accatctgaa aggcgatccc
     2401 ctctttgcag gtaatcttat ctttgtattc cttgcctgtg gcgcagcagg aactcttatt
     2461 ggcgctcctt ttgccgacag gtgggggcat agatttttta tcaggcttac catgtttctt
     2521 gcaacattga tcctgcctct catgtttgtt cccttcattg ccaaaagcta tcttctcttc
     2581 gtcgtattgg ggttggaggg actgattctc atatctacgt tttcggttac cgtcgtaatg
     2641 gcgcaacgac ttctgcccca tcgcctcgga atagcctcag gtcttatggt aggctttgcc
     2701 atcggcacag gcggtattgg tgttaccatc ttaggggtta tcgccgacaa cttcggcgtt
     2761 cctgttgcgc ttgaagcaat catggtcctc ccgttcattg gctttctttt aagccttgtc
     2821 gtaaaataca aggaatgagc cagaaaccga ttgtcaccat tgtagggcgg ccaaatgttg
     2881 gcaagtccac cctctttaac cgcctaatcg gatacaggaa agctataaca gaggacacac
     2941 ccggagttac ccgggaccgc aattatggtg aatttgaata tgccggcact gattttcgtc
     3001 tggtagatac gggtggattt gaaccaacaa aggaagaagg ttactttcct cttatgcgtc
     3061 agcagatcac catatctctc gaagaatctt ccatgattat tttccttctt gatgggaaag
     3121 aagggttgct gccccaggac atggagattg ccaaggggct gcgaagatat ggcaagccaa
     3181 tctttttcac ggtaaacaaa gtcgattctg gtaaacggga gttgcttacg ccagaatttt
     3241 ataccctcgg cacagaaaaa ctctatcctg taagctctct ccatgggttg ggtatagatg
     3301 aattgctcga agacctcgtt tcaacggcaa aacaggtgat gaatgataaa gatccgcaga
     3361 aaaccaggaa gcgtaggaaa cataccgatg atgtcataga gaaagattcc atatcagaag
     3421 ccatggatat ggaactatca acggagatta aaattgcttt tgctggaaga ccgaacacag
     3481 gaaaatcttc cattgcaaac cggctcctgg gttctgagcg gatgatagta agcaatttac
     3541 ccggtacaac acgtgatgca attgattcac aggtagcatt tcgtaacaga aacttgatcc
     3601 tcatcgatac tgctggcctc aggcgaaaaa gcaggattga agcaaaggtt gaagaacatt
     3661 ccgttgtatc ggccctgagg acgattgaac gggctgatgt ggtcaacctt attattgatg
     3721 caaatgaagg ggtaagccat caggatggtg gtattgccca tacgatcatt acacggggaa
     3781 aaggactctg cattgtggtg aacaagtggg atctcgttga aaacaaaata agcatggaag
     3841 aataccgcaa aatggtgcat gagaggatac cgcacacaag ctttgctcct gttatctgcg
     3901 tatctgcaaa aacagggaaa aacataaaga aaatcctcga atcagacctc agaatctata
     3961 gccagctcaa caggagaatt aagacatcag ccctgaacaa gacgctggag gagataattc
     4021 aggcgcagag ccttccgtac cagcacggaa aacgggtaaa tattatctat atgaaccagg
     4081 tcaaggttat gcctcccact tttgccctgt ttactaactt tcccaggcta atccctgagc
     4141 attacaaaag gtaccttgaa aattcgctcc gtcaaaaata tgggtttacc ggggttccga
     4201 tacggctttt actaaggaag aaatagttga ttcagatgcc ccatggtatg aattcaattt
     4261 atgatgtttg aggatccaat gagcttaaaa atacgcgtaa aaagattctt ctattactgg
     4321 tttcctatct tatgctttat ggccctgatc ttttttctat cttctctcca tggatcttca
     4381 atttcgcact ttggcttcct cgatgtgctt gtcaagaaag gggctcatat taccgggtat
     4441 gcagttcttt attttctcct ttttcgtgct tttcaatctc ttatgactcc ccgcagggct
     4501 ctctttgtat ctgcagtcat tgctgtgatt tacgccatat ctgacgaata ccatcagact
     4561 tttgtcccat tccgggaagg aacgatacgt gatgtcttca ttgattccat aggtgtcttt
     4621 cttgtctatc tctttctcag gcaaagatat aaccactgat ttgagcattc tttcattgtg
     4681 tagcctgttg ccaagattct ttctccccca tctttaccct tcatcatcct ctttctggcc
     4741 ttaatattgc aataattcct ggtatgataa gcagaccaat taacaaggag ccatggatga
     4801 gaattgtcga aaatgaaaag aaaccagctg tgagcgtgga agaattactc gatctgctcc
     4861 aggcaaagaa ggaaaaggag gcgagacatc cggaacgaca aaagtcattc tttcaaagca
     4921 tccctgttgc cacaatcata ctgggactcc taattgtagc cctgggggca atggtcatta
     4981 tactgaaatc tgacattgct tcactaaaaa atgacattac tgatttaaag aatctcaaaa
     5041 cccaggtagc ggctcttgac ccaaagcttc agattaccac ccttgaaggt aagctggagg
     5101 attttaagaa agaaagagag gtgataaagg gagaagtgac ccagttgagg ggagaaatgg
     5161 aagcgctgaa aaccgatcga aagaagggta agaagtaggc gattttaaaa tgaaggaggg
     5221 atggtagaaa tcttatcgct tcttacctga atataaattt atatcgtcag ttcaaaagag
     5281 actcgttttt cttgagccct atggtattca atcatctcct gggtggagat gaatttgcgg
     5341 gttgtgaacc gatatcgggc taggctctcg tcaaccatag aaaaatccac agagtctgct
     5401 gatttgttaa aactgggttc atttttctgg atgtatccag tttttgaact taaaaagctt
     5461 ttgtctacag acttctcacg gctgtggttt cccttcagta tactcccagc tgagtagatg
     5521 acgggggtga gggaatttcc tttatcaggt gttccttgaa gatcaacgat atcgctgaca
     5581 tactctgatt ttacattcag gctcaatgag ttcttttgat cttcgctgga taattttgat
     5641 accccttgag catcctctcg tttcttttca catggctccg gtgttatatc gataacctga
     5701 aacttatagg gtttaattct ggacgcgcct gtctcaacct ggaccctggt gacagacctt
     5761 ttgactgtaa tcacttcttc ctggagtctt gtaaaaagaa gcttcatttc tgcaacctac
     5821 ccaaatcaga tagatttact actgccccaa gctacaatag aatgtgacat tcatcacaca
     5881 tattttcagt atacacccaa tctcttgatt ctgcaatccc aaatctttag aatcgcctgt
     5941 tgcgatggtc tgcatccggt tattctttta ccccaaaact tttatttacc ttcaagaaac
     6001 ccttaacaaa acctctctgt gcagtagagt agatt
//
LOCUS       DAOOMC010000150         5909 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdus sp. isolate AMR_MDS_2685
            NODE_16119_length_5909_cov_6.083533, whole genome shotgun sequence.
ACCESSION   DAOOMC010000150 DAOOMC010000000
VERSION     DAOOMC010000150.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864760
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdus sp. (wastewater metagenome)
  ORGANISM  Syntrophorhabdus sp.
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae; Syntrophorhabdus.
REFERENCE   1  (bases 1 to 5909)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5909)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,729
            Genes (coding)                    :: 2,708
            CDSs (with protein)               :: 2,708
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 7 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5909
                     /organism="Syntrophorhabdus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16119_length_5909_cov_6.083533"
                     /isolate="AMR_MDS_2685"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2699756"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            610..933
                     /locus_tag="PK125_12190"
     CDS             610..933
                     /locus_tag="PK125_12190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB38904.1"
                     /translation="MFEVSEKALEKMRQAQQGQEGPLSIRVFKTEGGWKGPHLVMAFD
                     EEKENDEVFTMKGITFLIEKELYERSKPIYIDYTESALGPGYTLKSELMKGSIFECDN
                     IRNHC"
     gene            complement(1036..>3193)
                     /locus_tag="PK125_12195"
     CDS             complement(1036..>3193)
                     /locus_tag="PK125_12195"
                     /inference="COORDINATES: protein motif:HMM:NF012602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HPB38905.1"
                     /translation="VERVEMNKKTVAGICGICPGGCGVNVLLVDGVVEKITPIKGHPI
                     GVVCVRGTHSKEIIYSRDRLKHPLLRVGKKGEGSFKQITWDEAIEHIVQAFQKTKDKY
                     GAQAVMSYFGRGSFESNILDIFGTPDPTSQGVTGFLYPFGSPNGTGVSSICAVSYNLL
                     APLPTIGTPMHALYPDFENSNLIVIWGTNPTTDSPPDKVKKILSAKKQGAKVIVIDQM
                     RSAMAKLADEWIGIRSGTDGALALSLLHVIINEGRYDKDFVRDWTKGFEELRAYVQHF
                     PPEQVEKITRVPSYTIVRLARSISGTQGASLLMYTGLEYSNCGVQSIRAALSLWAITG
                     NLDTPGGLVFRPRSPTKFPRIRLEPPQGVKPIGADKYPYFCEVLKSAQFMEAPRAILD
                     GDPYPVRALLVFGASLLTSLPNPDLWKECFKKLDFMVVYDRFMTADAMYADIILPATT
                     NYENVGYHRHPDGYCQLRQRIIEPIEEARSGYTFLATLAKGLGYGDLFPLTDEERLRF
                     TFGSGPVSLVKLKQNPEGIHFDAGRREYQKYEKGLLRRDGEKGFNTPSRKVELLSSVL
                     EKYGYDGLPVYIEPKEGPLGNPELYKKYPLVFNSGARLQSAFRSQHLNIPGLLKIQPK
                     PQVLIHPTDAMQRGINNGDKVWVESPRGRVSFWAKVTEDVMTGQVEVNVGGGSPIHPV
                     EWREANTNYLTDYENRDPISGFPVYKALLCDVKKQE"
     assembly_gap    3194..3293
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            3476..5467
                     /locus_tag="PK125_12200"
     CDS             3476..5467
                     /locus_tag="PK125_12200"
                     /inference="COORDINATES: protein motif:HMM:NF013481.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016625 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, iron-sulfur
                     protein as acceptor [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase C-terminal
                     domain-containing protein"
                     /protein_id="HPB38906.1"
                     /translation="MRYAETGYNLEIDLATGNIQRVETDPKLMETHLGGLGTSVKLHW
                     DRGGLDVKPFDDENLFVVSSGLFNGTPAFSANRTVCTFISPQTHLLGYPMMGGFFSAE
                     LKHAGYDKIVFRNKSPKWVYLWIKNDKVEIRDASHLQGRGALETQDLIRKELKEPRAQ
                     VAAIGLAGENKCFTASIEQSRSSCSRLGGGAVMGDKKIKAIAVRGTKDINLARGEEFM
                     KLLDGVKDYINFRNANPIPDVMTILAGIGSPQEMVHTDEKWHTENFAWGNARVRRKDF
                     WNKENEANWTSTQLATVKRLISCYNCPQHCGGLVSFQGGPYYMMKCFGKLTYSMAAYV
                     DDLGFNFRILQRATEYGVDSFSTPQILAFAVELYEAGILTDKDFAGTSTYEACPSDNE
                     GRFFWLLDRVVRREGIGDILADGVYWAARKIGNGAEAFDHNTIKKHEQLPLKLGMMDP
                     IYYLMYTTNEKISITQIEGNFPQAAFPTMEQREAFVKDWPQIPDEKFKQYVLDWEPRG
                     EMANPYYPTPEIASEIVDWMEMLHNIDDALCLCAGMASFCLKPPYHINNYPQLISAAT
                     GMDLDREGLKKIANRSRNLHRALNNIRGMSRADEKPPEDHWKKRFPDIEENLLSTYYK
                     FKGWNNNGVPTRATLEKFDLGYVADELEKRGVLKDGQDN"
     gene            5451..>5909
                     /locus_tag="PK125_12205"
     CDS             5451..>5909
                     /locus_tag="PK125_12205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB38907.1"
                     /translation="MAKIIKKVKTIKVDADKCNGCRGCEIVCSGFHATPIWSSINPAR
                     SRIQVITDRLNNKWLPVFAGQYTAAECMGRNKYIIDGKEYEECDFCRASCPSRELFQE
                     PDSILPLKCDMCESIPPLDQPKCVEVCLNDVLTYEEREEEVEEDVKVEDVE"
BASE COUNT         1661 a         1222 c         1416 g         1510 t
ORIGIN      
        1 ctataattaa aattgcaaga attggatttt taaccaacga gtattggtaa ttagtaaaga
       61 ccatcttatc catagacaag atatgcttgt ctgagaagac accggaagta cttatagatt
      121 cccccacccg ggatgggtgg gggaaagggc agaaaacggg gcaatgaatc gatggaacat
      181 cgaatatcgc ccctcttgga ttgggcaatc tgttcgtcta tttatacatg ataaaatttc
      241 atatatgtgt tattataagt aacgattggt aattttaagt aatatatagt aactttatta
      301 ctaataacac gatgaaaata aaaagacaag aaaaaataaa tttttttggt ttcatggaaa
      361 aaagtgagat gatcaggtca gttttaaccc atccgtactt cactatgcat ttttcagttg
      421 ggcagcggtg tagacaaaaa tccattcaat ctgccttttc cggacagtta gtacaggtcg
      481 aggaaagagt gttggttggg ttagatctat gtgctgcccc tgagaaaggt tggcgaacag
      541 gcagataagg cgcttacgtc ttctggccgg gatagttgta attagtaaat ttataataag
      601 gagtatccca tgtttgaagt aagtgaaaaa gcattggaaa agatgagaca ggcccagcaa
      661 ggacaggagg gtcccctttc tatcagagtg tttaagactg aaggtgggtg gaaaggtcca
      721 catctcgtga tggcatttga tgaagaaaag gaaaatgacg aagtttttac aatgaagggt
      781 ataacattct taatagagaa ggaactctat gagagatcaa aaccgatata cattgactac
      841 actgagtcag ccttggggcc gggttacacg ctgaagtcag agcttatgaa gggcagtatc
      901 ttcgaatgcg acaacattcg caaccattgc taaggtaaca tcgatccgag aatacgagat
      961 tctggaaggc aacacgtgat agaggcgcag atccatgctc aggatcccat gatcctgtat
     1021 cttgtatgat ccactctact cctgtttttt gacatcgcaa agcaaggcct tgtatactgg
     1081 aaagccagaa ataggatcgc ggttttcata atcggtcagg taatttgtgt tcgcttcgcg
     1141 ccattccaca ggatggatag gactccctcc gccaacgttc acctctacct gtccggtcat
     1201 aacatcctct gttactttag cccaaaagct tacccgtccc ctgggggact ccacccaaac
     1261 cttatcacca ttgtttattc cccgctgcat tgcgtctgtg gggtgaataa ggacctgtgg
     1321 ttttggttga atcttcaaaa gtccgggtat attcaagtgc tgggatctga aggcagactg
     1381 aagtcttgca ccgctattga atacgagggg atattttttg tatagttcgg ggttgccaag
     1441 tgggccctct ttcggctcta tgtacaccgg cagaccatcg tagccgtact tctctaacac
     1501 gcttgaaagg agttccactt tccgggatgg tgtattaaac cccttttcac catctcttct
     1561 gaggaggcct ttttcgtatt tttgatattc tcgcctgcct gcgtcaaagt gtattccttc
     1621 cggattttgt ttcagtttca ccagggacac agggcctgac ccaaaggtga atcggagtcg
     1681 ctcttcatct gttaaaggga acaggtctcc ataacctagc cccttggcaa gtgttgctaa
     1741 aaaagtatat ccggatctgg cttcttcaat tggctcgatt attcgctgcc gaagctggca
     1801 atatccatct ggatgcctgt gataccccac gttctcgtaa ttggtggtgg cagggagtat
     1861 tatatccgca tacatggcat cagctgtcat aaacctgtca tagaccacca taaaatcgag
     1921 ttttttgaag cattctttcc acagatcagg attgggtagg ctggtcaaaa gcgacgcacc
     1981 gaaaaccaga agagctcgta cagggtaggg atcaccatcc aggatagccc tgggagcctc
     2041 catgaattgt gctgatttaa ggacctcgca aaaataaggg tacttgtctg caccaatagg
     2101 ctttaccccc tggggcggtt ccagtctgat acgggggaat ttcgtgggac tccgaggcct
     2161 gaacacgaga ccgcctggcg tatctagatt tcctgtaata gcccagaggc tcagggcagc
     2221 tcgaatgctc tgaactccgc agttgctgta ttcaagtcct gtgtacataa gtaacgaagc
     2281 accttgcgtg ccggatatgg accgggcgag tctcacgata gtataactcg gcactcgggt
     2341 aatcttttca acttgttctg gtggaaaatg ctgtacatag gcacggagtt cctcaaaacc
     2401 ttttgtccaa tcccggacga agtctttatc ataacgccct tcatttatga tgacatgcag
     2461 taggctcagg gcaagtgcac catctgtgcc tgaccgtata ccaatccatt cgtcagcaag
     2521 tttggccata gctgaccgca tttgatcgat gacgatcacc ttagccccct gttttttcgc
     2581 agaaaggatt ttttttacct tgtccggagg tgaatcagtt gtgggatttg tgccccagat
     2641 tacaatgaga ttggaattct caaaatcagg gtacagggca tgcatagggg tccctatggt
     2701 tggtagtgga gcgagaagat tataagacac ggcacaaatc gaagaaacac cagtgccgtt
     2761 gggcgagcca aaagggtata agaatcctgt tacaccctgg ctggtggggt caggagtacc
     2821 aaagatatcc agaatgttgg attcaaaact accacggcca aagtagctca taacggcctg
     2881 tgctccgtac ttgtcctttg ttttctggaa ggcctgaaca atgtgttcta tggcttcatc
     2941 ccacgtgatc tgtttgaagc ttccttcacc ttttttacct acccggagca aaggatgttt
     3001 cagtctgtct ctggaataga tgatttcctt tgaatgagtg cctcgaacgc atactacgcc
     3061 gataggatgg cccttgatag gtgtgatttt ttcaaccaca ccatcgacca gcaaaacatt
     3121 tacgccacat cctccgggac atatgccgca tatgccggcc acagtttttt tgttcatctc
     3181 aaccctttca accnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnccaaata
     3301 cgacgataaa aatatgcata taatgctaat aaagtatcga cactatcttg tacaggttgt
     3361 tgaaaaacag atatgtcgaa acatgaaaat tgttacatcg caggaatttg acagggaata
     3421 taaacaggca gcggattgta atgctgcaca gacagatatg taaaggggta aagatatgag
     3481 gtacgccgaa actggttaca atttggaaat tgatttagca acaggaaata ttcaacgtgt
     3541 ggaaactgac ccaaaattaa tggagaccca tctcggaggg ttgggcacca gcgtcaaact
     3601 ccattgggac agaggcgggc ttgatgtaaa gccgtttgat gatgaaaacc tatttgttgt
     3661 cagctcaggt ctttttaatg gcacacctgc gttcagcgcc aaccgtactg tatgcacttt
     3721 tatctctccc cagactcatc tactgggata tccaatgatg ggcgggtttt tttcagcaga
     3781 actgaagcat gctggttatg acaaaattgt atttcgaaat aaatcaccca agtgggtgta
     3841 cctatggata aaaaacgaca aggtggagat acgtgatgcc tctcacctgc agggcagagg
     3901 agctcttgag acccaggacc tcattcgaaa ggaattgaag gagccgaggg ctcaggtggc
     3961 agctatcggc ctggccggcg aaaacaaatg cttcaccgct tctattgagc agtcaagatc
     4021 aagttgcagc aggctcggag gcggtgctgt tatgggtgac aaaaagataa aagccatagc
     4081 tgtccgtgga acaaaagata tcaaccttgc cagaggagaa gagttcatga aactcctgga
     4141 cggggtaaag gactatataa attttcggaa cgcaaaccca atcccagacg ttatgacaat
     4201 tttggcaggt atcggatcgc ctcaggaaat ggttcatacc gatgaaaagt ggcatacaga
     4261 aaacttcgcc tggggtaatg ccagagttcg aagaaaagac ttctggaata aggaaaacga
     4321 agcgaactgg accagcaccc agttggctac ggtgaagcgt ttaataagct gctacaactg
     4381 tccgcagcat tgtggtgggt tagtttcctt tcagggtggc ccctactata tgatgaaatg
     4441 ctttggcaaa cttacatatt caatggcagc ctatgtggat gatctgggtt tcaacttcag
     4501 aattcttcaa cgtgctactg agtatggtgt agactcattc tcaactccac agattctggc
     4561 ctttgctgtc gagttgtatg aagccggtat tttaaccgac aaagattttg ccggtacgtc
     4621 aacgtatgaa gcgtgtccat cagataatga gggaagattt ttctggcttc ttgacagagt
     4681 tgttcggcgc gaaggaatag gggatatcct ggccgatggc gtgtactggg cagcaagaaa
     4741 gattggaaat ggcgcagaag ccttcgatca taacactatt aaaaaacatg agcagttgcc
     4801 cttaaaactg ggaatgatgg atcctattta ttatcttatg tatacaacca atgaaaagat
     4861 aagcatcacc cagattgaag ggaatttccc ccaggctgcc tttcccacaa tggagcagag
     4921 agaggctttt gtgaaggatt ggccccagat tcccgatgaa aaattcaagc aatatgtcct
     4981 tgactgggag ccacgggggg agatggcaaa tccatattat ccaacccctg aaatagccag
     5041 cgagattgtt gactggatgg agatgcttca taacatagat gatgcactct gtttgtgtgc
     5101 tggcatggca tctttctgcc tgaagccgcc ctatcacata aataattacc cgcaattaat
     5161 ctcagcagca acagggatgg atttggatcg agaagggttg aagaagatag ccaacaggag
     5221 cagaaattta cacagagcat tgaataacat acgggggatg agcagggccg atgagaaacc
     5281 accagaagac cactggaaaa agaggtttcc tgatattgaa gaaaatctgt tgtccacata
     5341 ttacaaattc aaaggatgga acaataatgg tgtacccacg agagcaacat tggagaaatt
     5401 tgatctgggg tatgttgctg atgaattaga aaagagaggt gtactgaaag atggccaaga
     5461 taattaagaa agtaaaaacg atcaaggttg atgctgataa atgcaacggc tgccgcggtt
     5521 gtgaaatagt ctgctctggc tttcatgcca cacccatttg gagcagcatt aatccggcaa
     5581 ggtcccgtat tcaggttatc actgacaggt tgaacaacaa gtggcttcct gtattcgcag
     5641 gtcagtatac tgctgccgag tgtatgggtc ggaacaaata cataatcgac ggcaaggaat
     5701 atgaagaatg tgatttctgc agggcctcct gtccgtccag agaactgttt caggaacctg
     5761 attcaatcct tccactcaag tgtgacatgt gtgaaagcat ccccccgttg gaccagccaa
     5821 aatgtgttga ggtatgcctt aatgatgttc tgacctacga agaacgggaa gaagaagtgg
     5881 aagaagacgt aaaggtcgag gacgtagag
//
LOCUS       DAOOMC010000151         5902 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdus sp. isolate AMR_MDS_2685
            NODE_16153_length_5902_cov_7.056097, whole genome shotgun sequence.
ACCESSION   DAOOMC010000151 DAOOMC010000000
VERSION     DAOOMC010000151.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864760
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdus sp. (wastewater metagenome)
  ORGANISM  Syntrophorhabdus sp.
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae; Syntrophorhabdus.
REFERENCE   1  (bases 1 to 5902)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5902)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,729
            Genes (coding)                    :: 2,708
            CDSs (with protein)               :: 2,708
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 7 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5902
                     /organism="Syntrophorhabdus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16153_length_5902_cov_7.056097"
                     /isolate="AMR_MDS_2685"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2699756"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..314)
                     /locus_tag="PK125_12210"
     CDS             complement(<1..314)
                     /locus_tag="PK125_12210"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HPB38908.1"
                     /translation="MSKILIIVEYVVVTIILSAIALLVSLFDKQGRALHRMAQFWAKI
                     HLMTSGIQVSVTGLENISSPPYIFMCNHQSALDIFSLLNSLPVHLNWTGRLLRSEKIS
                     SAL"
     gene            complement(311..1087)
                     /locus_tag="PK125_12215"
     CDS             complement(311..1087)
                     /locus_tag="PK125_12215"
                     /inference="COORDINATES: protein motif:HMM:NF013793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon monoxide dehydrogenase accessory protein
                     CooC"
                     /protein_id="HPB38909.1"
                     /translation="MKVAISGKGGVGKTTLAGVMARILAKQGYKVLAIDADPDSNLAS
                     AIGVDQKKLDSAKPLALMKEFIEERTSSKKGTYGSFFKLNPRVDDIPERFAVDKDGIK
                     LVILGNIQQGGGGCFCPESVLLKSLLSYIIIERDESVIVDMEAGLEHLGRGTAEYVDA
                     LLVVVEPGKRSFQTAYQVKRLSQDLGIKNLYIVGNKIMDISDDELMRNSLEGFTVLGT
                     ISYNEKVIEADKLGVSPFDIDEKIRTEVGYIIEALRNRVT"
     gene            1337..3250
                     /gene="htpG"
                     /locus_tag="PK125_12220"
     CDS             1337..3250
                     /gene="htpG"
                     /locus_tag="PK125_12220"
                     /inference="COORDINATES: protein motif:HMM:NF003555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HPB38910.1"
                     /translation="MTIERMEFKTEIKQLLDLMIHSLYSHKEVFLRELVSNASDAIDR
                     ARYESLTNPEIAEKDGGWHIRITPDKATNTLTVSDNGIGMSKEEIITALGTIAHSGTR
                     EFITALQSKEVKDNPELIGQFGVGFYSSFMVADKITVVSRRAGQDPGKGVRWESTGDG
                     SFTIENIEKLTKGTDITLHLKDDEKKYLQEWEIKSIVKKYSDFIEHPIVMEVEREKQS
                     DIDKSQRVKIREDETLNSQKAIWLKDKNDINEDEYNEFYKHISHDFAKPSKVIHYKAE
                     GTSEFTALLYTPSKAPFNILYRDYKIGPTLYVKRVQIMDHCEELIPPYLRFIKGVVDS
                     SDLPLNVSREILQNNRQVDIIKANITKKVLDTLQDMKNEEKDSYAEFYKEFGRVLKEG
                     IHYDFERGETIADLLLFSTTKTEEGKFRTLEEYVKDMKEDQQDIYYATATSMIEVLKS
                     PYMEAFSKKDYEVLVLLDDIDDFLFSGFEYKGKKFKSISKGSIELDKSDESKKEKTKK
                     EYGKLMDMIKDVLKDHVKDVRMSGRLIDSACCLVADEGDPDPHMEKLLKAMGQEVPAA
                     KRVLEINPDHPLFAAMNRIFEKNRSEPVLTDFIFLLYDQALLLEGSKPQDPSAFTQKI
                     AQLMVESLEKTGT"
     gene            3360..4019
                     /locus_tag="PK125_12225"
     CDS             3360..4019
                     /locus_tag="PK125_12225"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HPB38911.1"
                     /translation="MRESIRQIVKIFADTFPVEEPIYEFGALQLPGFEDFADLRSFFT
                     GKSYVGCDMREGRGVDKVLNLHEIAIPDETVGTAITCDTLEHVEFPHKALLEIHRILK
                     PGGVVFLSTVLDFRIHDSPADYWRFTPDGLRSLLRPFSFFFVGSAGRESFPHTVIAIG
                     VKGSNFMSDSLERFLPRFEEWKKAWINPKGKSWKNSAYLFIPPVLLGLDRRIAKLFKK
                     S"
     gene            4135..4641
                     /locus_tag="PK125_12230"
     CDS             4135..4641
                     /locus_tag="PK125_12230"
                     /inference="COORDINATES: protein motif:HMM:NF039354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6125 family protein"
                     /protein_id="HPB38912.1"
                     /translation="MDLRIFQSMEKNELISYLEFLFWHYRVVDAFWFIKVAEQFDQEI
                     AERLNESVWSRVPRMAAEDLVKRFGIQEKGLKGFLKALKLFPWTVLVEYQIEEKEDEI
                     IISVPHCPTQEARLRRGLDEFVCKYMHEGEFNSFAHAIDPTIKVECMFGPPDPHPKDM
                     FCKWRFTI"
     gene            4699..5808
                     /gene="ald"
                     /locus_tag="PK125_12235"
     CDS             4699..5808
                     /gene="ald"
                     /locus_tag="PK125_12235"
                     /EC_number="1.4.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000286 - alanine dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HPB38913.1"
                     /translation="MIIGVPKEIKEGEKRVALTPAGVHVLVHDGHSVVVETGAGMGSR
                     LSDEEYILEGATIASKKDLFDKAELILKVKEPLESEWPYLREGKILFTYLHLASSRNL
                     TNGLLRTGITGIAYETVQDKLGNLPLLIPMSEVAGRMAVQLAVHFLEAEYGGRGILLS
                     GVPGVPPAEVVILGCGVTGYNAAKVAEGQGAHVTIIDINYSRLKYADDVLHGKVMTLF
                     SNPYMIKQAVANADVLIGAVLIPGARAPVLVTEEMVSEMKPGSVIIDISVDQGGSVET
                     TRPTSHAEPIYKIHDVIHYAVPNMPASVPLTSTAALTNATLPYIRAIASKGLKKAAEH
                     DPSLAAGINLARGVLTHPAVAESFNMKCVSWKKVV"
BASE COUNT         1813 a         1258 c         1268 g         1563 t
ORIGIN      
        1 agtgcactcg atattttttc gctcctcaat agtctccctg tccagttcaa atggacaggg
       61 agactattga ggagcgaaaa aatatcgagt gcactctgat gattacacat aaatatgtac
      121 ggaggcgatg agatattttc aaggcctgtt accgacacct gtatgccact tgtcataaga
      181 tggatttttg cccaaaattg agccatacga tgcaatgctc taccctgttt gtcaaaaaga
      241 gatactaaga gtgcaattgc tgaaagaatg attgtaacaa ctacatattc gacaataatg
      301 agaatcttgc tcatgtcact cgatttcgaa gggcttcaat aatgtatccc acctctgtcc
      361 ttattttttc atctatatca aagggagaca cacctaactt gtcagcctcg ataactttct
      421 cattatatga aatagttcca agaactgtaa aaccttccag gctattcctc atgagctcat
      481 catcagaaat atccatgatt ttgttaccta caatgtaaag gtttttaata ccaaggtcct
      541 ggctcagtct tttcacctgg tatgctgtct gaaagcttct cttaccaggc tcaacgacaa
      601 ctaacaaagc atctacgtat tcggccgttc cccgaccaag gtgttcaaga ccggcttcca
      661 tgtctacaat caccgactca tccctttcaa taatgatata agaaagaagg cttttgagta
      721 acacgctttc tgggcagaag caaccgcccc cgccctgctg gatgttgcct agtataacca
      781 gtttgatacc atccttatca acagcaaatc tctctgggat gtcatcaacc cgggggttca
      841 acttaaagaa tgaaccatat gttccttttt tgctggacgt acgttcttcg ataaattctt
      901 tcatgagggc aaggggtttc gcagagtcaa gctttttctg gtccactccg attgcactcg
      961 caaggttcga gtcggggtct gcatcaattg caaggacttt gtagccttgt ttggcaagaa
     1021 tccgtgccat aacgccagca agggttgtct tacctactcc accttttcct gatatggcaa
     1081 ccttcatatc aacctccgac tattgtcacc aacaatctta actgtaatga tgatcccggt
     1141 taaaactgag cggatatggt caattctctt gaataagtct tttcccggac acacagacta
     1201 ttatagcatg aatcatggga cacgcaaggc gttttgatat tgactaacca gacctcagga
     1261 attatataaa tactgcattc gcattttttg tatcattcag cgaacacaac tatttagttg
     1321 aacgaggtga acttatatga cgattgaacg aatggaattc aaaactgaaa tcaaacaact
     1381 cttggatctc atgatccatt ccctttactc ccataaagag gtgttcctca gggaactagt
     1441 ttcaaatgca tcagacgcca ttgacagggc cagatacgaa tcccttacta acccggaaat
     1501 cgcagaaaag gatggaggat ggcatataag aataactcct gacaaggcca ccaacactct
     1561 tacagtgagc gacaatggta tcggcatgtc aaaagaggag atcattaccg ctcttggcac
     1621 tattgcccat tcagggacaa gagaatttat tacagccctt caaagtaaag aagtcaaaga
     1681 taatccggag ctcatcggcc agtttggcgt aggtttttat tcctccttca tggtggcaga
     1741 caaaataaca gttgtctcgc gcagagccgg tcaggaccct ggaaagggag tacggtggga
     1801 gtcaacaggc gatggctcat ttacaataga aaacattgaa aaattaacga aaggaacaga
     1861 tattactctc catctgaagg atgacgaaaa aaaatatctc caggaatggg agataaaaag
     1921 tattgtcaaa aaatactcgg acttcataga gcatcctatt gtcatggaag tcgagcggga
     1981 gaagcagagt gacattgaca agagccagcg agtaaaaatc agagaagatg aaacactcaa
     2041 ctcacaaaag gcaatctggc tcaaagataa aaatgacata aacgaagatg agtacaatga
     2101 gttctataaa cacatctcgc acgattttgc taagccatca aaagtaatac attacaaagc
     2161 agaaggcaca tcagagttca ccgcccttct atacacccct tcgaaggcgc ctttcaacat
     2221 tctttatcgc gactacaaaa taggaccgac cctgtatgtg aaacgggttc aaatcatgga
     2281 tcattgcgaa gaattgattc ctccttacct gcgttttatc aaaggtgttg tcgattcttc
     2341 tgaccttccc cttaatgttt caagagaaat cctgcaaaac aacaggcagg tggatatcat
     2401 taaggcaaac atcacaaaaa aagtactcga tacccttcag gatatgaaaa atgaagaaaa
     2461 ggactcgtat gctgagtttt acaaggaatt cggaagggtg ctcaaggaag gaatacatta
     2521 tgatttcgaa agaggagaaa ccattgcaga ccttctcctt ttctccacaa ctaagactga
     2581 ggaaggcaaa tttcgaaccc tggaagaata cgtaaaagac atgaaagagg accaacagga
     2641 tatttattat gccactgcca catccatgat tgaagttctg aagtcacctt atatggaagc
     2701 attctccaaa aaggattatg aggttctcgt tttattagat gacatagatg attttctttt
     2761 cagtggtttc gaatataaag gaaaaaaatt taaatccata tcaaaaggca gcattgaact
     2821 cgataaatcg gatgaaagta aaaaagagaa aactaaaaag gaatatggca aactcatgga
     2881 tatgatcaaa gatgttctga aagatcatgt gaaagatgtg agaatgtcag gccgtcttat
     2941 tgattcggca tgttgcctcg ttgcagatga aggtgatcct gatccacata tggaaaaact
     3001 tctcaaagct atggggcaag aggtacctgc cgcgaagaga gttctggaaa tcaacccgga
     3061 tcatcctttg tttgcagcca tgaaccgaat ttttgagaag aacaggagtg agccggttct
     3121 aacagatttt atttttcttc tgtacgacca agcactctta cttgagggat caaaaccgca
     3181 ggatccatcc gcctttacac agaaaatagc ccagctgatg gttgaaagcc tggaaaaaac
     3241 aggaacgtaa cattatttcc gctcattcct ggttgatttc gatcccacca tagtatataa
     3301 ccatacaggc atgaagaaga gaattactca ttcatgccaa tttaccgccc ggatagattt
     3361 tgcgcgaatc aatacggcaa atagtcaaga tatttgcaga tacatttcct gtggaagaac
     3421 caatctatga gtttggggct ttacaactcc caggttttga agattttgcg gacctcagat
     3481 cattcttcac ggggaaatcc tatgtcggtt gcgatatgcg agaaggacga ggggttgaca
     3541 aggtactcaa ccttcacgaa atagcaatac ctgacgagac tgttggcaca gcaattacat
     3601 gtgacacact ggaacatgtg gagtttccac acaaagccct tttggaaatt catcgcatac
     3661 tcaagccggg gggtgtggtt tttctcagca cagtcctcga tttccgaatc catgattcgc
     3721 ctgcagatta ctggcgtttc acaccagatg ggctgaggag tctccttcgt cctttttcgt
     3781 ttttcttcgt gggatcagca ggccgcgaga gttttcccca cactgtcata gcaattgggg
     3841 ttaaaggatc aaattttatg agtgactctc ttgaacgatt tttgccaaga ttcgaggaat
     3901 ggaaaaaagc gtggataaat ccaaaaggaa aatcgtggaa aaacagtgca tatttgttca
     3961 tcccacctgt ccttctgggt ctcgacagaa gaattgcaaa acttttcaaa aaaagctgat
     4021 gcattgagtc ttcagcgaga attacattct ttcttcctga tttattccat cattgacacc
     4081 ttgtaacttt gtgataaaat ataacttctt gtaaaaaaat aaggagagag ataaatggac
     4141 ttaagaatct ttcaatcaat ggaaaaaaat gaactcatca gttatctcga gttcctcttc
     4201 tggcattaca gggttgtcga tgcgttctgg ttcattaagg ttgctgaaca gttcgaccag
     4261 gagattgcag aaagactcaa tgaaagcgtc tggagcagag tgccccgaat ggctgcagag
     4321 gatttagtca aacggttcgg gattcaggaa aaagggctaa aaggttttct caaggcttta
     4381 aagctctttc catggactgt tcttgtggaa tatcagatcg aagaaaaaga agacgagatc
     4441 atcattagtg ttccccactg tcctacccag gaggcaagac ttaggagagg acttgatgaa
     4501 tttgtttgca aatacatgca tgagggagaa ttcaattcct ttgcccatgc cattgatcca
     4561 accatcaagg tagaatgtat gttcggtccg ccagaccccc acccgaaaga tatgttttgc
     4621 aaatggagat tcacaatata aaggttcaca agctacagaa caagagtaaa atctgaataa
     4681 agaataagag aggaagagat gattatagga gtaccaaagg aaattaaaga aggagaaaaa
     4741 agagtagctt taacaccggc cggagttcat gtcctggtac atgacggaca ttccgttgtt
     4801 gttgaaacag gcgcaggcat gggcagcagg ttgtccgatg aagaatatat tttggaaggt
     4861 gccacaatag cttccaagaa ggatcttttt gacaaagctg agttaatcct gaaagtgaaa
     4921 gaaccactgg aatcagaatg gccatatctg agagaaggca aaatactctt tacgtacctt
     4981 caccttgcgt catcaaggaa tctcacaaat ggccttttga ggactggcat aactggtatt
     5041 gcatatgaga ctgttcagga taaactcgga aacctccccc ttctcatacc tatgagtgaa
     5101 gttgcaggaa ggatggctgt tcaactggct gtgcattttc tggaagctga atatggaggt
     5161 aggggaattc tgctgagcgg agttcctggt gtaccacctg ccgaggttgt gattcttggg
     5221 tgtggcgtaa ccggttataa tgccgcaaaa gttgccgaag gtcagggtgc acatgttaca
     5281 atcatcgata tcaattacag ccggctcaaa tatgctgatg atgtcttaca cggtaaggtt
     5341 atgaccctct tctcaaaccc gtacatgatt aaacaggctg tcgccaatgc ggatgtttta
     5401 atcggtgctg tattaatccc tggagccagg gctcctgtac ttgtaacaga agaaatggtt
     5461 tcggaaatga aaccaggctc tgtcattatc gatatatccg ttgatcaggg aggctccgtc
     5521 gagacaacac ggcccactag ccatgctgaa cccatctaca aaatccatga tgtgatccat
     5581 tacgcagttc cgaatatgcc tgcttcagtg ccactaactt cgactgctgc cctaacaaat
     5641 gcaacacttc cttacatcag ggctattgca tccaagggac tgaaaaaggc tgctgaacat
     5701 gacccttctc ttgccgcggg cattaacctt gcccgtggtg tccttactca cccggcagta
     5761 gccgagtcct ttaatatgaa atgtgtttca tggaagaagg tggtttagac caatgtactt
     5821 ctgacgccca tgaccattac atgctcttaa agaattgcat cgggccctgc gccagctgtt
     5881 ttccgtaccg tttacaatcc gg
//
LOCUS       DAOOMC010000152         5611 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdus sp. isolate AMR_MDS_2685
            NODE_17313_length_5611_cov_6.298236, whole genome shotgun sequence.
ACCESSION   DAOOMC010000152 DAOOMC010000000
VERSION     DAOOMC010000152.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864760
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdus sp. (wastewater metagenome)
  ORGANISM  Syntrophorhabdus sp.
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae; Syntrophorhabdus.
REFERENCE   1  (bases 1 to 5611)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5611)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,729
            Genes (coding)                    :: 2,708
            CDSs (with protein)               :: 2,708
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 7 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5611
                     /organism="Syntrophorhabdus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17313_length_5611_cov_6.298236"
                     /isolate="AMR_MDS_2685"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2699756"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1307..2248)
                     /locus_tag="PK125_12240"
     CDS             complement(1307..2248)
                     /locus_tag="PK125_12240"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HPB38914.1"
                     /translation="MPDKSGYLFIITAALLWAISGTASKFIFSTGILPIELVQCRTAG
                     SAIFLACILGVKKPSLLMINLKKTGYFILLGALLAVTQYTYLFAISKTLVAVAILLQY
                     QAPLVVALYMFFVLREKPSITTVIAIFCALLGCYLTVGAYNLNILLMSKTGIIAGLCS
                     AVTFALYTITSEFGMRHYSPWTVLLYAFSVAAIILNIIYPPLHAFGRSHTFVTWWWIF
                     FITLFGTILPFAFYNLGIQRIRPTHASITATLEPIAAAIISYAFLGEILEQWQIIGAI
                     LVITAIVILQQKPQKKMATNSLNELTKTQGLKKNVER"
     gene            complement(2385..3086)
                     /locus_tag="PK125_12245"
     CDS             complement(2385..3086)
                     /locus_tag="PK125_12245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB38915.1"
                     /translation="MEKKPRQVKKRSSVPATEKTTLNPIKLGQVLVRAGRIRSEDLEQ
                     ALREQDASKKRIGEILIEKGFIKPEELTHGLNVQSLFTSSGMSAGQSFDSVLEGQFTH
                     KHEEKKTGIKVKAQVPIRSHLAVIYQAPSVILSKADIKAGYIILKNASHIAIKSNDKK
                     GFMVVFKGLKWPFKEAQVFGFTHDVHITHDGASVQQPYKKNIQNVELSYRFILSDNAK
                     AGRHPWPLSIALQPV"
     gene            complement(3163..4317)
                     /locus_tag="PK125_12250"
     CDS             complement(3163..4317)
                     /locus_tag="PK125_12250"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HPB38916.1"
                     /translation="MNSRKRELITSFLRTFIPAVTHAHQYTTSHQLTVAAINNAYLRL
                     IEAIGTDQSIAFMIIDDRIIMHDEPLEDTLFSNRFVRIFKNRGIQHIRILQNITEEEI
                     TALIEALTSRSPLPENLDYLPNVQLGTVSIGFKPRDGDGRLDTYGESASQFNTIQVNS
                     FNLSSDIYHAVKHNENLPLMEIKNLVTDLIAAIRHESSVLLSFSPLRVLDEYTFTHST
                     NVSILNLAQAMALGIKGEELHEIGVAALLHDTGKLYVPEEVLNKPGKLTDTEWESIRQ
                     HPQKGAEYLLENPGIPPLAVVVAYEHHMQYDNSGYPQVSKDWRQNICSQMTAISDFFD
                     AMRTKRIYRDDLETNIIADQMAKMSGTALNPILTRNFLVLIEQMIRGLRL"
     gene            complement(4314..>5611)
                     /locus_tag="PK125_12255"
     CDS             complement(4314..>5611)
                     /locus_tag="PK125_12255"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HPB38917.1"
                     /translation="KELYIPPNILNILERLKRESAAQDSSEPDNPLNRISQDELSEKF
                     NIIFKEDELDRFVPLDYQKILHDVVLADTISAPELTQVRQLKDTLSNQSIHLHLTAVV
                     VDIISKGNSDEPPHIVQALKNCCMFLLSIGDFHGVSDIYEMVMKKVHTSTINNDVKKT
                     SGILEIFADDDFIDYVLDGRAQWGKEKDFYIVELIKKVGEPFVEPLLDRMASEEDRTL
                     RYFYLDLLGELGASVKGPVIKRLKDNRWYYVRNLIILLRNLNDPSVLPVLHNLLDHPH
                     PKVRHELMQTLIKFNDPVAERIILQEMDSPDVGRCLKAITLAGMTRNRLVSLKLQEFL
                     KQKGFGKTILPVKKASVYALGEIGDPTTLPTLQDILKSRTFFRRHAAMNLKLEIIDSL
                     KKYPVGDVSPILRELASSGPQPLVNHARTVMKDMKVGLP"
BASE COUNT         1564 a         1070 c         1301 g         1676 t
ORIGIN      
        1 gaattacctt ggcctttttg gtcatatagt ggggatatac catggtcctc gacagcaaaa
       61 gaactataga tcggattgac tgaatataga cgataagttt gaacatgaag agtgtaaaag
      121 gtttttcata agcctgcagg atgaaggcgt gaggagaatt ctagatatat gacaaaaata
      181 tggtcaaaag aacacgcatt gccaaaaaaa agacagcttc cacagcaatt cttgacgatg
      241 ataaagggat tacacgtatc tactcttcac agtgctaagc ccaccagaag atactttgag
      301 cttccttaaa agtagaaagt cagttggttt tgagacgaag tattgtttaa gtagcgttaa
      361 cactgagaaa gttgagaatg taatggtaaa tgagttagta ggaaggaatt gttttctgtg
      421 atgtgaggaa agctgaaatt ttgccaccta catctggtgt tagagtatct ttagaaaaaa
      481 taggctacat tgctgcatgg catcttcagt ctaatcgtct actgctaaag agtgtgacac
      541 ctcgtttatg tttgctggtc tcttaaaacg tctatatatt tttggtctag atcgaaatat
      601 actctttatc ccccttgaaa ggaattgaaa tgagaattat gttacaacca gaacaagccc
      661 tcatcccccg agacttggga tatctaatcc cccccaaaaa caaacccggg tctcgggttc
      721 ttttttattc aaacccatgt tgtgtgcttt ctgctaaagg agaggaaagc acacaacata
      781 gcggatattt taggagggat aaaggcttct gcttcccggt tttgcaagag gacgatgcta
      841 tccttgctga aatacccggt aaccgagggt aagctcatcc gccacatctg aggtaaagga
      901 ggcgaggtgg ttctcgagga gccacttcac cagggatggt ggtaagagaa agggttgatc
      961 cagatcctag ggtttgaatg ttgtgttcat ggaatacaca ctagaggggt aggaatatct
     1021 tattctacct tgttttgaaa gggattcgca tggagattat tcggacaagc acctagtgtt
     1081 cacgagattt tggcgactgt cttctatttt ttttgatgtt agtgataagg acttagcagt
     1141 tgtgtatagt tgtgaatccc ttacttaatc acagcccaca tttgcatctg tcaatgtttt
     1201 ggtgttttgt cttgagttct tacattgtta gatgtttctg gagcgttttc gtccgttttt
     1261 aaaaatgggc tcttgttcct aagattaatg ggttcctcat ggtgtatcac ctctctacat
     1321 tctttttaag cccctgagtt tttgttagct cattaagaga attggttgcc atcttttttt
     1381 gaggtttttg ctgcagaata acaatggctg ttattacaag aattgctcca atgatctgcc
     1441 attgttcaag gatttcaccg aggaaagcat aggagattat tgctgcagct ataggctcaa
     1501 gagttgccgt aatgcttgca tgggttggtc gaatccgttg tattccaaga ttgtaaaaag
     1561 caaaaggcag tatggttcca aaaagtgtta taaaaaagat ccaccaccat gttacaaagg
     1621 tatgtgagcg gccaaaggca tgcaaaggcg gataaatgat gttcagtatg atggctgcaa
     1681 cagaaaaagc atagagcaga actgtccacg gtgaatagtg gcgcatccca aattcgcttg
     1741 taatggtata aagtgcaaag gttactgcag agcaaagacc tgcgataatc cctgttttgc
     1801 tcattagaag aatgttaagg ttgtatgcac caacagtgag gtaacaacct aacaaagcgc
     1861 agaatatggc aataactgtg gtaatagaag gtttttcccg caaaacgaaa aacatgtaaa
     1921 gagcaacaac cagcggagcc tgatattgga gaagaatagc aaccgcaacc aatgttttgc
     1981 ttatcgcaaa aaggtatgtg tattgggtaa ctgcgagtag agcgccaagg aggatgaagt
     2041 aaccagtttt ttttagattg atcattaaaa gcgaaggttt tttaacccca agaatgcaag
     2101 caaggaaaat tgcagatcct gctgttctgc attgaacaag ctcgatgggc aatattccgg
     2161 ttgaaaaaat aaactttgat gctgttccag atattgccca gagaagtgct gcagttataa
     2221 tgaaaagata tccactttta tcaggcatcg agaatattgc cgccagataa ggcattgaaa
     2281 gtcaagggaa atatccctca tatggagaaa tgatataatt tggtagaaag cttgtactta
     2341 gaaatggtat gtaaaacagg gatatgcaac cagttatata ttttttatac gggttgtaat
     2401 gcaatagaga gaggccacgg atgtctccct gcctttgcat tgtcagacaa aataaacctg
     2461 tagctcaatt ccacattctg tatatttttt ttatatggtt gttgtaccga tgctccgtca
     2521 tgagtaatgt gtacgtcatg ggtaaatcca aaaacttgtg cttccttgaa gggccacttt
     2581 aagcccttaa aaactaccat aaatcctttt ttgtcattgc tcttgattgc aatgtgtgaa
     2641 gcgtttttta gaataatata tccagccttt atgtcagcct tgctcagaat aacagatggg
     2701 gcctgataga ttaccgcaag atgagatcgg ataggtacct gtgccttgac ttttattcca
     2761 gttttctttt cttcatgctt atgggtgaac tgaccttcaa ggactgaatc aaaagattgt
     2821 ccagcagaca ttcctgaaga agtaaataga gactgaacat tgaggccatg ggtaagttcc
     2881 tcaggcttaa taaacccctt ttcaataagg atctcgccaa ttcttttttt tgaagcatcc
     2941 tgttctcgga gggcctgctc caaatcctca cttctgatgc gtcctgccct tacgagaacc
     3001 tgcccaagct taattgggtt tagtgttgtt ttttcagtag caggcacact agatcttttt
     3061 tttacttgac gtggtttctt ttccacagaa taactcattt acccatttcc ttttaaatcc
     3121 ttatcgttca aaataatcaa aacttaatta ccaccccatc attcaaagac gtaagccgcg
     3181 aatcatctgt tcgataagaa caagaaagtt tcttgtgagg attgggttga gtgcggttcc
     3241 tgacattttt gccatctggt ctgcaataat gtttgtttcg aggtcgtctc gatatattcg
     3301 ttttgtcctc attgcatcaa agaagtcaga tattgcggtc atttgactac agatattttg
     3361 tcgccaatct ttagatacct gagggtagcc ggaattatca tactgcatgt gatgctcgta
     3421 tgctacaact acagctaatg gggggattcc agggttttca aggagatatt cagcaccttt
     3481 ctgggggtgc tggcgtatgc tctcccactc agtgtcggtc agctttcccg gtttattcag
     3541 gacttcttca ggtacgtaaa gtttacccgt gtcatgtaaa agggctgcga cacctatctc
     3601 gtgaagctct tcacccttga ttccgagcgc cattgcttgg gcaaggttca gaatagacac
     3661 attggtggaa tgggtaaatg tatactcatc gagaacgcga agtggggaaa aagaaagaag
     3721 cacagaagat tcatggcgaa tggctgcgat cagatcagtt accaggttct tgatctccat
     3781 gagggggagg ttttcgttgt gctttaccgc gtgatagata tctgatgaaa ggttgaaact
     3841 gttcacctgg atagtgttga actgtgaagc gctttcccca taggtatcaa gtcttccatc
     3901 gccgtcccgg ggtttgaaac caatagatac tgttccaagc tggacattgg gaaggtaatc
     3961 aaggttttcg ggaaggggag aacgactggt aagggcttcg ataagggcgg ttatctcttc
     4021 ttcagtgata ttctgcaaaa tacgtatgtg ttgaatgccc cggtttttaa agatccggac
     4081 gaaacgattg gagaaaaggg tatcttccag gggttcatcg tgcataatga tcctgtcgtc
     4141 gataatcatg aatgctatcg attgatccgt accgatggcc tcgatgaggc gcaagtaagc
     4201 attattaatt gcagctacgg ttaattggtg agaggttgta tactggtgtg cgtgggtcac
     4261 tgctggaatg aaggtacgga ggaaactggt gatcagttct cgtttccggg aattcatggc
     4321 aacccgacct tcatatcttt catgacagtc cgcgcatggt tcacgagagg ctggggccca
     4381 gagcttgcaa gctctctgag aataggggac acatctccca ccgggtattt ttttaaggaa
     4441 tctatgattt caagtttgag gttcatagct gcgtgacggc ggaagaacgt tctcgatttc
     4501 aagatgtcct ggagagtagg gagggtagtt ggatctccaa tctcgccaag ggcataaaca
     4561 gaagccttct tgacgggaag tattgtcttt ccgaaacctt tctgtttcag aaattcttgg
     4621 agtttcagtg atacgagcct gtttcgtgtc atacctgcaa gagtgatcgc ctttaagcac
     4681 cttcctacat caggtgaatc catctcctgt agtatgattc tctctgcaac aggatcattg
     4741 aacttgatca gggtttgcat taattcatgc cgtacttttg ggtggggatg gtcaaggagg
     4801 ttgtgaagga ctggaagaac cgaaggatca ttgaggttcc tcagaaggat tataaggttt
     4861 ctcacataat accaccggtt atcctttaac cttttgataa caggcccttt cactgacgca
     4921 ccaagttcac caaggaggtc aaggtagaaa tagcggaggg tccgatcttc ttcggaagcc
     4981 atgcggtcaa gcaagggttc aacaaagggt tccccaacct ttttgataag ttccacgata
     5041 tagaaatctt tttccttacc ccactgagca cgtccatcaa gtacataatc gataaagtca
     5101 tcgtcggcaa aaatctctaa aatgccactg gttttcttca catcattgtt aatggtcgaa
     5161 gtatgtactt tcttcatcac catttcgtaa atgtctgata ccccatgaaa atctccgatg
     5221 gagagtagaa acatacagca gttcttgaga gcttgcacaa tatggggtgg ctcatcggaa
     5281 ttcccctttg aaataatatc aaccacgact gcagtcaaat gcaggtgtat gctttggttt
     5341 gagagggtat cttttaattg tcggacctgt gtgagttcag gtgccgagat tgtatccgcg
     5401 agaacaacat cgtgaagtat tttctggtaa tcaagtggta cgaaccggtc taattcatct
     5461 tccttaaaaa tgatgttgaa tttttcggag agttcgtctt gtgagatacg gttaagtgga
     5521 ttatcaggtt cactagaatc ctgggcagct gactctcttt ttaatcgctc tagaatattg
     5581 agaatgtttg ggggtatata gagttctttt t
//
LOCUS       DAOOMC010000153         5568 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdus sp. isolate AMR_MDS_2685
            NODE_17493_length_5568_cov_7.170687, whole genome shotgun sequence.
ACCESSION   DAOOMC010000153 DAOOMC010000000
VERSION     DAOOMC010000153.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864760
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdus sp. (wastewater metagenome)
  ORGANISM  Syntrophorhabdus sp.
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae; Syntrophorhabdus.
REFERENCE   1  (bases 1 to 5568)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5568)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,729
            Genes (coding)                    :: 2,708
            CDSs (with protein)               :: 2,708
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 7 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5568
                     /organism="Syntrophorhabdus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17493_length_5568_cov_7.170687"
                     /isolate="AMR_MDS_2685"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2699756"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(591..4088)
                     /locus_tag="PK125_12260"
     CDS             complement(591..4088)
                     /locus_tag="PK125_12260"
                     /inference="COORDINATES: protein motif:HMM:NF024904.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HPB38918.1"
                     /translation="MRIDELQLRSFGPFTDINVVFKKREGDFHILYGRNEAGKSSALR
                     ALKALLFGIPHQTTDNFLHENAKLRIGGQIRSSDGDHISFLRRKGTKNTLLDNKEKPI
                     EEAALYRFLPGVTEEIFETFFGIGHDSLVQGAQSILQEGGAVGQSLFSAGMGGVDLRA
                     VLQEFDYEADLLFRPRGQTQAINKAINDYADLKKTISEATLLSRKWLEHTQELKKVLG
                     EREGLSKKIELVQHEKSRAGRLQQTMPDIALREKLMTTLMDLGDVVIVDDDFGRKRRE
                     YLEQLRIANETKERSSSEVDQLKRGMDLIIIPEGILDQAETISDLHQRMGSHRKGLQD
                     LGRLQGNIQQLNQEIEILASEIKPGISVDACLELRPIAVVRAKIQDLGSKHQALLDAI
                     ERSSKDIWKSEGNLTKAKEELRSLDAPKSIDALKAAISRAQKRGDLTSSLEEARKHCD
                     AEERQIAIDLSRLPLWSRGIGELEAIAIPSSETVDRFESTFHEITTKLTSLDEKEKEV
                     EAELAAVGLKIETLRMSGAVPTEDELLKVRGRRDLGWSLVLRSWIDNETIVEEATAFD
                     PNDDLPKAYEKSVVQADDVADRLRREADRVAQNANFIAQQSAIREALVDIEENRKKLN
                     DRLGETESAWNEQWEHAGIRPLSPKEMRSWIVKQGALIQRANKSRELRQTYDQIVRLI
                     EEHGAALTACLDDLGQKYDINKPFEALLVDCQSVVDSVEKVNRKRADLEKKINELEDD
                     LKTLHQNDEALKKQHTTWQSGWKGAVAKLGLEPETSPVEAYAVLSKLDELFKKVDEKT
                     SIERRIFGIKRDASEFSSDVEKLVTRIATDLVNLPIEQAVAQLNSSLSKAKTDAARLD
                     DLENRLGKQEKILREAEETIRITLKRLDSLCIKVGCARYEDLEEIEQKSSLKKSIQEK
                     IDSTETRLLGVGGGMSIEELIEEAKDIDADSLPGIISEIDRQLEELQTERSDLDQRLG
                     SMKASLAQMDGSGKAAEAAEQSQQVLASMREKVERYVHLRLCSMILTREIERYRSENQ
                     GPLIKRASQLFSALTLNSFAGLTTDFNDKDELIIVGVRPTGEKISVSGMSDGTRDQLY
                     LALRVASLEKYIDTNEPMPFIVDDILIRFDDERAMAALNILSDLSKKTQVLFMTHHAR
                     LVELVKGDSFSGNPEIHTL"
     gene            complement(4085..5347)
                     /locus_tag="PK125_12265"
     CDS             complement(4085..5347)
                     /locus_tag="PK125_12265"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair exonuclease"
                     /protein_id="HPB38919.1"
                     /translation="MKFIHAADIHLDSPLIGLERYDGAPIDEVRGATRKALQNLVERA
                     VHEKVDFVLIAGDLYDGDWKDYNTGLFFVSQMTKLRDSGIKVIIVTGNHDAASQITKN
                     LRIPDNCRMLSVHKPESVVFESLGVAIHGQGFSTRAVTDNLVAHYPKARTGLFNIGLL
                     HTSLNGREGHEPYAPCTLDELLSRGYGYWALGHVHKREIVHQEPWVVFSGNSQGRNAR
                     ETGEKGCMLVSVSDGILTDIKFISLDIFRWSVIDVDVTGLSEIDSILNTLDKALKREL
                     QVNDNMPLALRIRLSGSSRLHGKILARAEHLKNEIRGCAIDVGYGNIWVEKVCIETSG
                     MSDPETLMMRDDALGGLLRSISDLQYDGKSLDDMLGELGDLFRKLPAEYLDDMEAIHP
                     ERKKNLLHVVEDVKHMLLTRILSSGGGI"
     gene            5337..>5568
                     /locus_tag="PK125_12270"
     CDS             5337..>5568
                     /locus_tag="PK125_12270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB38920.1"
                     /translation="MNFISIFPRLTLLTQETVEVLPQKRNQDMGEAEDVLERLTTNTL
                     RTRYMFDKHRTLLCQPIAGGDLLASNRGASDTR"
BASE COUNT         1375 a         1443 c         1101 g         1649 t
ORIGIN      
        1 tttatgccac ttcagataat aatttttatg tgaactaatg gccgtttagg acgcgccact
       61 ctgctttcat gacccgtcat tgatgagcag gcttttcata ataacctccc tgttcgaata
      121 taaatctatt atataatatt ctgtccttcc tgtgcacttc tgaatcaatt ttctcgagac
      181 tatccggcag gtgagagctg gcagggcaag tatgcagatc ttcctattac ctgttaattt
      241 cagaaaagta tcggggttga cgatttgaat gttcttgtat tctttaaggt ctgtaaaatg
      301 ccacttcgtt cattacctgc ccaagatttt gtctggccct taaagtagga agtttcttaa
      361 tcgtgacata cactccttga tgttatagta actatatcaa tggaactact tattataact
      421 gatgttccga tattgtgaag gcggaacttt tgttccaggt cggtatcgcg aaagaccttc
      481 gatgacgctt cacggttaat tttttttgcc ttagcccata tcgattgatt atgtattttt
      541 tcaccgagta tttcagaagg ctattttgtg gttgtctgcg acacgcttca ctaaagcgta
      601 tgaatctccg gatttccgga aaatgaatct ccctttacca gctcaacaag tcgcgcatgg
      661 tgtgtcatga ataacacctg cgtcttcttt gataggtctg acaggatgtt cagcgcagcc
      721 atggccctct cgtcatcaaa ccgtatcaat atatcgtcta caataaacgg catgggttcg
      781 tttgtatcaa tatatttttc aaggctggca actcgaaggg cgaggtagag ttgatccctt
      841 gtgccgtcgc tcataccgga aacactgatc ttctctcctg taggacggac cccgacaatg
      901 ataagctcat ccttatcatt gaaatccgtt gtcaaaccag caaaagaatt gagagttaag
      961 gcagaaaaga gttggcttgc cctttttata aggggaccct gattttcact tcggtatcgt
     1021 tcaatctccc tggtgagtat catggaacac aggcgcagat gcacatagcg ttctactttt
     1081 tctcgcatgg aggctaaaac ttgctgggat tgctcggctg cttctgcagc ctttccgctc
     1141 ccatccattt gagccaaaga agccttcata ctccccagcc gttggtccag atcagacctt
     1201 tcagtctgca actcctccaa ctgcctgtca atttcagata ttatccccgg caaagaatca
     1261 gcatcaatgt cttttgcctc ttcaataagc tcttcgatgg acatccctcc tccaactcca
     1321 agaagtcggg tctctgtcga atctattttt tcttgaatgg actttttcag agatgatttt
     1381 tgctcgattt cctccaaatc ttcatatctt gcgcagccta cctttatgca aagggaatca
     1441 agccgcttca gggtaatcct tatcgtttct tctgcttcac ggaggatctt ttcttgcttg
     1501 cctaaccgat tctccagatc atccaacctg gcagcatctg tttttgcttt gctgagcgat
     1561 gaattcaact gagccacagc ctgttctata ggcagattga ccaagtcagt ggcaattctt
     1621 gttacaagct tttctacatc tgatgaaaac tcgctggcat cgcgcttgat tccaaatatc
     1681 cgccgttcaa tggatgtttt ttcatcgacc ttcttgaata attcatcgag tttggagaga
     1741 actgcatatg cttcaacagg agatgtttct ggctcaaggc caagtttagc aacagcacct
     1801 ttccagcccg actgccaagt agtgtgctgt ttcttgaggg cctcatcgtt ttgatgaagg
     1861 gttttaaggt catcctctaa ttcatttatc tttttctcaa ggtcggctct tttgcgattt
     1921 accttttcta cagaatcgac aacagactga caatcaacca ggagggcttc aaaaggcttg
     1981 ttgatgtcgt acttctgacc aagatcatca agacaggcag tcagggctgc gccatgttcc
     2041 tctatgagcc tgacaatttg atcgtatgtc tggcgtaatt cccttgattt gttggctctc
     2101 tggatgagcg ctccctgctt gacaatccac gacctcatct ctttcgggga taatggcctg
     2161 attccggcat gttcccattg ctcgttccag gcagactctg tttctcccag tcgatcattc
     2221 agctttttcc tgttttcttc aatgtccaca agagcttccc gaatcgcgct ttgttgggca
     2281 ataaagtttg cattctgggc tacgcgatct gcttcccttc gaagcctgtc agccacgtca
     2341 tctgcctgca ccacgctttt ctcatatgct ttcggaagat catcattcgg atcaaaagca
     2401 gttgcttctt ccacgatggt ttcattatca atccaagaac gcagtaccag gctccagccc
     2461 aggtctcttc ttcccctcac tttcaaaagc tcatcttcag tgggtaccgc tccggacata
     2521 cggagagttt ctatctttag ccccacagca gcaagttcgg cttctacttc cttttccttc
     2581 tcatccagac tggtcagttt tgttgtaatc tcatgaaagg tagattcaaa tcgatcaact
     2641 gtttcagacg acggtattgc tattgcttcc aactccccta taccccgtga ccacaggggc
     2701 agcctgctca ggtctattgc aatctgcctt tcctcagcat cacaatgctt gcgagcttct
     2761 tcgaggctcg acgtcaggtc accccgtttc tgagcacgcg aaatagcggc cttcaatgca
     2821 tcaatgcttt tcggagcatc aagcgaccgt aattcctcct ttgcttttgt taaatttcct
     2881 tcagatttcc aaatatcttt actcgatctc tctatggcat ctaaaagagc ctgatgctta
     2941 ctgccaaggt cctgaatttt cgcccgcact acagcaatag ggcgaagctc caaacaggca
     3001 tccaccgata tgccaggctt tatttctgaa gcaagtatct caatctcctg attcagttgc
     3061 tgaatgtttc cctgcagcct gccaagatcc tggagccctt tgcggtgact ccccatccgt
     3121 tggtggagat ctgatattgt ctcggcttga tcgagtatcc cttcagggat gataatcagg
     3181 tccattcctc gttttaactg atccacttca ctggatgaac gctccttagt ctcattcgct
     3241 attcgaagct gttccagata ttctcttctc ttccgtccaa agtcatcatc aacgataacc
     3301 acatcaccaa gatccataag agtggtcatt aatttctctc gcaaagctat atcaggcata
     3361 gtctgctgca agcgccctgc cctgcttttc tcatgctgca caagttctat tttcttcgat
     3421 aaaccctctc tttccccaag aactttcttt agttcctgag tgtgttccaa ccattttctg
     3481 ctaagaaggg ttgcttctga aatagtcttc ttgagatcag cataatcatt gatggcctta
     3541 tttatagcct gagtctgccc tctgggacgg aaaagcagat cagcctcata atcgaactcc
     3601 tgaaggacag ccctaagatc aactccaccc attcctgcgg aaaagagact ttgaccaacc
     3661 gcaccgcctt cctgcaagat gctctgggcg ccctgtacca atgaatcatg accaatgcca
     3721 aaaaatgttt caaatatttc ctcagttact cctggaagaa atctatacag ggcagcctcc
     3781 tctataggtt tttccttgtt gtcaagcaag gtgttcttgg ttccttttct cctcagaaaa
     3841 gagatatggt caccatcaga actcctgatc tggccgccga tcctcaattt tgcattctcg
     3901 tgaagaaaat tatcagtcgt ttgatggggt atcccgaaaa gaagcgcttt caacgccctg
     3961 agggctgacg acttaccagc ctcattgcga ccatagagga tatgaaaatc gccttcacgc
     4021 tttttaaaaa ctacattaat atcagtaaat gggccaaatg agcgtagttg cagttcgtct
     4081 atcctcatat gccacccccg gaagacaaaa tcctggtgag cagcatatgc ttcacatctt
     4141 ccacaacgtg caataggttc ttcttccgtt ccggatgaat ggcctccata tcatccaaat
     4201 attcagccgg cagcttgcga aaaagatccc caagctcccc gagcatatcg tcaagcgatt
     4261 ttccatcata ttgcagatcg ctaatagaac gcaaaagtcc accaagcgca tcgtctctca
     4321 tcattaatgt ttcaggatca gacattcctg aagtctcgat gcaaactttt tcaacccaga
     4381 tgtttccata acccacatcg attgcgcatc ccctgatctc attcttcaga tgctctgccc
     4441 tggccagaat cttcccgtgc agtctcgatg atccggacag acgaatacgc agggcaagtg
     4501 gcatgttatc attgacctgc agctctcgtt tcagggcttt atccaatgtg ttaaggatgc
     4561 tatcaatctc tgataagcct gtaacatcca catcaatcac cgaccaccgg aaaatgtcaa
     4621 ggcttatgaa tttgatatca gttagtatgc catccgacac agaaaccagc atacagccct
     4681 tttctcctgt ctcacgcgca tttcttccct gagagtttcc agaaaatacc acccatggct
     4741 cctgatgaac aatttctctt ttgtgaacat gtcccagggc ccagtaccca taaccacgag
     4801 atagtagttc atccagggta caaggtgcat agggttcatg accttcgcga ccatttaaac
     4861 ttgtatggag aagcccgata ttgaataaac ctgtgcgggc ttttggatag tgggctacaa
     4921 ggttatcagt aacagctctg gtcgaaaatc cctgtccatg aatggccacg ccgaggcttt
     4981 caaagacaac cgattcgggc ttgtgcactg agagcattct gcaattgtcc gggatacgaa
     5041 gattcttggt aatctggctt gcagcatcgt ggtttccggt cacaataatt actttgatac
     5101 ctgagtcccg taacttagtc atctgggaca cgaaaaataa ccctgtgttg taatccttcc
     5161 agtctccgtc ataaaggtcg cccgcaatca acacaaaatc gactttttca tgaacagccc
     5221 gctcaacaag attctgtagc gcttttcgtg ttgcgccccg cacttcatct atgggagcac
     5281 catcatacct ctccagccct atcagtgggc tgtcgagatg aatatcagca gcatggatga
     5341 atttcatttc tatcttcccc agactaacac tattaacaca agaaacagtt gaagttctac
     5401 cacaaaaaag aaatcaagac atgggcgaag cggaagatgt actggaaaga ttaacaacca
     5461 ataccctgcg cacccgatac atgtttgata agcaccggac attactttgt cagccaattg
     5521 ccggtgggga cctattggct tcaaaccgtg gagcttctga taccaggc
//
LOCUS       DAOOMC010000154         5359 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdus sp. isolate AMR_MDS_2685
            NODE_18402_length_5359_cov_6.903281, whole genome shotgun sequence.
ACCESSION   DAOOMC010000154 DAOOMC010000000
VERSION     DAOOMC010000154.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864760
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdus sp. (wastewater metagenome)
  ORGANISM  Syntrophorhabdus sp.
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae; Syntrophorhabdus.
REFERENCE   1  (bases 1 to 5359)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5359)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,729
            Genes (coding)                    :: 2,708
            CDSs (with protein)               :: 2,708
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 7 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5359
                     /organism="Syntrophorhabdus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18402_length_5359_cov_6.903281"
                     /isolate="AMR_MDS_2685"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2699756"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..665)
                     /locus_tag="PK125_12275"
     CDS             complement(<1..665)
                     /locus_tag="PK125_12275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB38921.1"
                     /translation="MKLVQFCVEPKTGLDDQAVATLVDYVLGPKQHKELALPKTLEST
                     GAYYEFDMRSTLHQFMEYFYTARIPSSITRPSSLRSSLWTAPSGDTQKLPNSWKTSFP
                     AENPIIIHGIERAANTPDLNTGVYHEYDMKRTLILLNYRGHQTVLSISKQISTSNVGK
                     KGFILGNDSDWNYYYTEEPGSTITGLGWAKSYIYDYFSVTVFVEPSSGSIGVKTGIFQ
                     WLRA"
     gene            complement(799..1818)
                     /locus_tag="PK125_12280"
     CDS             complement(799..1818)
                     /locus_tag="PK125_12280"
                     /inference="COORDINATES: protein motif:HMM:NF013921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HPB38922.1"
                     /translation="MPRKNRIDAPGAIHHVIVRGIGNSPIFKDSQDCENFISRLGRLL
                     CETTVSCYAWALMKNHAHLLLRSGSLPITSLMRRLLTGYAQQFNRRHNRSGHLFQNRY
                     KSFLCEEDPYLLELVRYIHLNPIRAGVVKEMAALERFRWCGHGVIMGKRKQDWQDSSF
                     ILSLFHENRSTAKKLYNSFVEKGIALGKRPDLVGGGLIRSAGVWIDVQDLRTSRERFS
                     SDERILGSSDFAASILKKANEDYGKRIGAETKQFNLEKIITAVCKHFDLKPNHVDSPS
                     KKPHITRARAIISSIAFDILRLKGVDIARRLNLTPTAISKLSAKGRADQSVNEISELI
                     FEQNE"
     gene            complement(1880..3181)
                     /locus_tag="PK125_12285"
     CDS             complement(1880..3181)
                     /locus_tag="PK125_12285"
                     /inference="COORDINATES: protein motif:HMM:NF015316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein transport protein"
                     /protein_id="HPB38923.1"
                     /translation="MGKVLIGCLLPLAIHLYFLSLAFGNGFDIYEQSAKAVGMAGAIT
                     AQADDPSAIFFNPAGITQLEGTQISAGACLIRPTMRFQSSGNPIMGTYPGETSKIKDH
                     TWVIPNAYVTHKVNEKVAIGIGTFAHFGLGVEWPKKWEGRFTPGATKTILRTTSISPV
                     VALRLSEDLSIGMGPYIQHFDIELNNLVFLAAPSPPITPGNNRAQTADAKLTAKEWDW
                     GWQAGFRLKIIKGLTFGAAYLSNVRHKISGGEQELKSLADGNVVVRQGFSGKFTLPAT
                     LRLGLAWQRGPWTIEAGAQWTEWTSYKTLKAVFDNGTSLTSPKNWHNAWLWRIGSQYR
                     VNKYLDLRAGFLYDETPIPSATLDPLVPSGDRMGYCGGIGLHFDKLTFDIAYNYIHDE
                     KRKWNNPSGDVKVGPLSITRVTGKFENAYAHILAMNVTYRF"
     gene            complement(3688..4059)
                     /locus_tag="PK125_12290"
     CDS             complement(3688..4059)
                     /locus_tag="PK125_12290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB38924.1"
                     /translation="MGTINRNFKIINVILLGFFAVLLTFCGMGTTKIGNITGHPRDYA
                     GKEVIISGEVTEIFSLFIIRYFTVRDGTGEITVVTDRPLPAKGEHIKIRGIVKEAFSL
                     GTQSTLVLVESSKKMPGKGTD"
     gene            complement(4117..4689)
                     /locus_tag="PK125_12295"
     CDS             complement(4117..4689)
                     /locus_tag="PK125_12295"
                     /inference="COORDINATES: protein motif:HMM:NF024937.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4126 domain-containing protein"
                     /protein_id="HPB38925.1"
                     /translation="METILAVITGIGLSAACGFRIFVPLLALSLASNYGLITLASGFE
                     WIGGYYAMVAFSSATVLEVIAYYVPWLDNIMDAIASPVSILAGTIATASVITDLPPSL
                     KWMLAIIAGGGIAGLLQGATTALRVKSSLFTGGTGNPAVSTLEFIGSAVTAILAIILP
                     LIGIILVVAFVVFVLIKVGRSFFKKMKAGS"
     gene            complement(4772..5320)
                     /locus_tag="PK125_12300"
     CDS             complement(4772..5320)
                     /locus_tag="PK125_12300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB38926.1"
                     /translation="MSLKPKIFLNRGADLIERRKGGGRLVFLGLPFFLVGLFVTQIPF
                     GFIPVELEARPLVMAILWPLGPLFAGAGFILMFSRSGLAINRRTCVAIQWWGLLIPMK
                     KKTYSLDSFTKVRIELRTGDKNSPDVFIISLTNTSAQSLFYIADLFDYHKALTAAEDL
                     AQFMGKPLENLVVDLQQVEPGV"
BASE COUNT         1474 a         1205 c         1187 g         1493 t
ORIGIN      
        1 gctcgaagcc attgaaagat acctgttttt acaccaattg acccggaact cggctccaca
       61 aaaacagtga ctgaaaagta atcgtaaatg tacgacttgg cccaccctag accagttatg
      121 gtggagccag gttcctctgt gtagtagtag ttccagtcac tgtcattgcc aagaataaat
      181 ccctttttcc ccacatttga tgtgcttatc tgttttgata tggagagtac tgtctgatgt
      241 cctctgtagt taagaagaat gagggttctc ttcatatcgt attcatgata aacaccggtg
      301 ttcagatcag gggtatttgc tgcccgttct atcccgtgaa tgatgatggg attctctgct
      361 ggaaaagatg tcttccaact gttaggcagt ttttgcgtgt ctccagaagg tgctgtccac
      421 agggatgaac gaagagatga aggtcgtgtg attgaagatg gaattcgggc agtgtaaaag
      481 tactccataa actgatgtaa agtgctcctc atgtcgaatt cataataagc tcctgtagat
      541 tcaagcgtct tgggaagagc cagttccttg tgctgcttcg ggcccaacac ataatccaca
      601 agggttgcaa cagcctgatc gtcaaggccg gtttttggct caacgcaaaa ttgcaccaat
      661 ttcatggccg cttgattaac agagagggga agatttttcg aagaggatgc cgatacactt
      721 tgatgcatca gagtcaaaac cattatcaat acgatgcaaa acaactttgg ggacgctctt
      781 gaaatgttga aattattttt attcattttg ttcaaaaatc agttcgctaa tctcattaac
      841 tgattgatca gcacggcctt tagcggataa tttcgatatt gcggtagggg tcagattcag
      901 ccgccgcgct atgtctacgc cttttaatct taatatatcg aaggctatag aggaaatgat
      961 tgcccttgcg cgggtgatat gcggtttctt tgaagggctg tcgacatgat ttggtttcaa
     1021 atcaaaatgt ttacaaacag ccgtgatgat cttttccagg ttaaactgtt ttgtctcagc
     1081 gccaatcctt ttcccatagt cttcgttcgc tttcttgaga atagatgcag caaaatcact
     1141 actcccgagg atacgttcat cgcttgagaa acgttccctg gatgtccgca aatcttgaac
     1201 atcaatccag acgcctgccg agcggataag gcctcctcca accagatcag gtcttttgcc
     1261 cagggcaatg cctttttcaa caaacgagtt gtacaatttt tttgcagtgg atcggttttc
     1321 atggaaaaga gacaatataa acgaagaatc ctgccagtct tgttttcttt tccccatgat
     1381 tacgccatga ccgcaccagc gaaatctttc gagggcggcc atctccttta ccacacccgc
     1441 acggattggg ttaaggtgga tatatcgtac aagttcaaga agataagggt cttcttcaca
     1501 gaggaacgat ttgtaacggt tctgaaaaag gtgaccagat ctgttgtgcc ttcgattaaa
     1561 ctgctgtgca taacccgtaa gcaaacgcct cattaaggat gtgatgggta aagatccgct
     1621 ccgaagaagc agatgggcgt gattcttcat tagcgcccat gcatagcagg atacagtggt
     1681 ttcacacaag agtcgaccaa ggcgggaaat gaagttttca cagtcttgag aatctttgaa
     1741 aatgggagaa ttcccgatgc ctctgacgat gacatgatgt attgcaccgg gtgcatctat
     1801 tcgattcttt ctgggcacgg gcaataatta cataaaatga ttaagatgtc aacatttcaa
     1861 gaacgtcccc tatgctcttt taaaaacgat aggtcacgtt catcgcgagt atatgggcat
     1921 atgcgttttc aaatttaccg gtaacgcggg tgattgataa aggtcctacc ttgacatctc
     1981 ccgaagggtt gttccattta cgtttttcat catggatata attataggca atatcgaagg
     2041 ttaatttgtc aaaatgtaac ccaatgcctc cacaataccc catccggtct cctgacggca
     2101 caaggggatc aagagttgca ctcggtatag gggtttcatc gtacaggaac ccggccctta
     2161 ggtcaaggta tttatttacc ctgtactggg atcctatgcg ccacaaccag gcattatgcc
     2221 agttcttcgg gcttgtaaga gatgtgccat tatcaaaaac agccttcaat gttttgtagc
     2281 tcgtccattc tgtccactga gctccagcct caatagtcca cggacctctc tgccatgcca
     2341 gccccaaccg cagggtggcg ggtagggtaa attttcccga gaagccctgt ctgacgacaa
     2401 cattcccatc tgcaaggctt ttgagctcct gttccccacc tgaaatcttg tgtcgaacat
     2461 tgctcaaata ggctgccccg aaagtgagtc cctttataat tttgagtcga aatcctgcct
     2521 gccatcccca atcccattcc tttgccgtaa gctttgcatc tgccgtctgc gctctgttgt
     2581 tgcctggtgt tattggtgga gaaggcgcag caagaaaaac gagattgttg agttcaatgt
     2641 cgaaatgctg gatataaggg cccataccta ttgataagtc ttctgatagt ctgagtgcaa
     2701 caaccggact gatagaggtt gttctcagga tcgttttggt tgcgccaggg gtaaaacggc
     2761 cttcccactt ctttggccat tcaacgccga gtccaaaatg ggcaaaagtc ccgatgccga
     2821 tagctacctt ttcatttact ttatgggtta cataggcatt aggtataacc caggtgtgat
     2881 ctttgatttt agatgtctct ccagggtagg ttcccatgat aggattacca ctgctctgaa
     2941 atcgcatcgt tggcctgatc aggcatgcgc cagcagatat ctgggttccc tccagctgtg
     3001 ttattcccgc cgggttgaaa aatatggccg aaggatcgtc tgcctgggct gtgatggctc
     3061 cggccatgcc cactgctttg gcgctttgct cgtaaatgtc aaaaccattt ccaaaagcaa
     3121 gagacagaaa atatagatga atcgctaacg gtaatagaca cccaatgaga accttgccca
     3181 tatccacacc tgtaaaatca tatgcatgtg atgactcagg gacgtagatc tatttttgat
     3241 caaaaacaaa atgaccaaac ccgaaaaacg cctgatggat aagtttagca gtatgttctg
     3301 gcaagttcta cagatttcct caaaaagaaa aagatttttg taaagcaatt acttttagat
     3361 aaatacagtc cttgggttca catctttccc ggcccgtatc ttgtagatga tcttttttgg
     3421 cagtgttttg cttcggtgtt aagagatact taggcatgat ttgaaaaaat attgaaatgt
     3481 gttctttgat aagagccaag tcgaggattt gccggtcact gtatcttcag atcttgtatg
     3541 tttcggcctt tttactaaaa ttccggccag aattaaggaa tctttttctg ttagcagatg
     3601 atctaacatg gccaaactgc atttttcgtg taattataaa atgttaacgt aagaaagaag
     3661 aagaatatgt gaaaactgca ggacttctca atcagtgcct ttacctggca tttttttgga
     3721 actctctaca aggacaagcg ttgactgtgt tcccaaagaa aaggcctctt taacgatccc
     3781 tctgatcttg atgtgttcac cttttgctgg aagcggtctg tcagttacaa cggtgatttc
     3841 cccggttcca tcccgtacgg tgaagtacct aatgataaaa agcgagaaaa tctcagtcac
     3901 ctcaccggag ataataacct ctttgccggc gtaatccctt gggtgacctg ttatattgcc
     3961 aatctttgtt gtgcccatcc cacagaatgt tagtaatacg gcaaaaaatc ccaacaaaat
     4021 aacattaatg attttgaagt ttctatttat agtgcccacg acagcccctt ttatctcacc
     4081 ataccacagg tcatgaatcg tttcaaaata aaataattag ctccctgctt tcatcttttt
     4141 aaagaaactg cgtcccacct ttattaatac aaagacaacg aaggctacaa ccaggataat
     4201 tccgataaga gggagtatga ttgcgagaat ggcagtaacg gccgaaccaa tgaattccag
     4261 ggtagatact gcgggatttc ctgtaccgcc tgtaaaaagt gaggatttta ccctgagcgc
     4321 ggtagtggcc ccctggagca gccctgcaat accgccaccg gctatgattg cgagcatcca
     4381 tttcaatgat ggtggtagat cagtaatcac cgatgctgtt gcaatagtgc ctgcaaggat
     4441 tgagaccggt gaagcaatag catccattat gttatcaagc catgggacgt agtaggcaat
     4501 tacctcaagg acggttgccg aggaaaaagc taccatagca tagtagccgc ctatccattc
     4561 aaatcctgac gcaagggtga taaggccata atttgaagcc agactgaggg caaggagggg
     4621 gacaaatatc ctgaacccgc atgctgcact cagacctatg ccggttatga ctgcaagtat
     4681 cgtttccatt tgatccatca taatctatta atttgccagc aaattttaat cattgtcctt
     4741 tattttcgag ttggggtcaa caggaacata cctatacgcc tggctctacc tgttgaaggt
     4801 caaccactaa attctcaaga ggtttgccca tgaattgggc caagtcttcg gcagccgtga
     4861 gtgccttatg atagtcaaag agatctgcta tgtagaaaag actctgagcg cttgtatttg
     4921 tcaggctgat tataaaaaca tctggagagt ttttatcgcc ggtccgaagc tctattctga
     4981 ccttggtaaa actgtccaag ctgtatgtct tctttttcat gggaatgagg aggccccacc
     5041 attgtatcgc cacgcatgtt cggcgattaa ttgcaagacc gctgcgactg aacatgagga
     5101 tgaatcctgc tccggcaaaa agaggtccta aaggccacag gatagccata acaagaggcc
     5161 gtgcctcaag ttcgacaggg ataaaaccaa aaggaatttg cgtgacgaaa agaccaacga
     5221 gaaaaaaggg caggccaaga aagacaagac gccctccgcc ctttcttctc tcaatcaaat
     5281 ctgcgccacg gttcaaaaaa atcttcggct ttaaagacat gcattcattc tcgaacaatc
     5341 cgcactaatt tgcaatgaa
//
LOCUS       DAOOMC010000155         5254 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdus sp. isolate AMR_MDS_2685
            NODE_18873_length_5254_cov_6.126370, whole genome shotgun sequence.
ACCESSION   DAOOMC010000155 DAOOMC010000000
VERSION     DAOOMC010000155.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864760
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdus sp. (wastewater metagenome)
  ORGANISM  Syntrophorhabdus sp.
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae; Syntrophorhabdus.
REFERENCE   1  (bases 1 to 5254)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5254)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,729
            Genes (coding)                    :: 2,708
            CDSs (with protein)               :: 2,708
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 7 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5254
                     /organism="Syntrophorhabdus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18873_length_5254_cov_6.126370"
                     /isolate="AMR_MDS_2685"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2699756"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            122..781
                     /locus_tag="PK125_12305"
     CDS             122..781
                     /locus_tag="PK125_12305"
                     /inference="COORDINATES: protein motif:HMM:TIGR01470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional precorrin-2
                     dehydrogenase/sirohydrochlorin ferrochelatase"
                     /protein_id="HPB38927.1"
                     /translation="MNSKHSKLSIKSNIYYPIFLDITKKPCLVVGGGRVAERKVSVLL
                     KFNGIIRLVSPQISNTLKRYGDKGRIHISLKEYGEEDLDGVALVFAATNVESLNERIY
                     NDAVKKNIPANVVDNPSLCDFIVPSIVKKDPIIIAISTSGMLPFLSKRLRKDMAEGIT
                     RDYVRYANIVGRLRKLLIMEIKDKKLRQSLMKEIMGTDVSVINNMGFKKAKETFFGSE
                     K"
     gene            781..1593
                     /gene="ccsB"
                     /locus_tag="PK125_12310"
     CDS             781..1593
                     /gene="ccsB"
                     /locus_tag="PK125_12310"
                     /inference="COORDINATES: protein motif:HMM:TIGR03144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome biogenesis protein CcsB"
                     /protein_id="HPB38928.1"
                     /translation="MFILFLIALCLYLVSTIFYLLFLAIDKKGIEALGHFSLLSGFIV
                     HFISTCFRYFQAGYTPITNLHESLSFLSLCMAGFFLFLKRSYKISILGSIIVPFLSLV
                     FIWALWLPSDIKPLAPILQSYWLPVHTIFAFIGNSIFFISFFVSILYLSLERGIKRKK
                     FSPISTRFPSLEVLDSINYRCMSYGFPFLTIGIITGSLWAGMAWGSYWSWDPKETWSL
                     ITWIVYAILIHNRLTIGWRGRKTAYMMIAGFFSILFTFLGINFLVGGLHSYL"
     gene            1705..2991
                     /gene="hemA"
                     /locus_tag="PK125_12315"
     CDS             1705..2991
                     /gene="hemA"
                     /locus_tag="PK125_12315"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: protein motif:HMM:TIGR01035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008883 - glutamyl-tRNA reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HPB38929.1"
                     /translation="MPIAVFGLNHNTAPIEVRERLFVSEETIPRLVRELQESGVDENI
                     VLSTCNRTEFYFSCKNLDSALDKFYQALSRHFSIEKEWLEKYTYLLTDEDTYRHLFLV
                     ASGLDSMVVGEPQILGQVKDAYRLATDCNTSGFFLDKVFHKTFNVAKRIRTETKVGYN
                     PVSISAMAIELAKKIFGELKDKKTLVIGAGEMCEIALRYFKKEGVGDIYITNRTFHNA
                     QKLADETTGIPRPFNDLQDLLEKVDMILSSTGSERPLISRELVSVVMKKRKQKPLFFI
                     DIAVPRDIEPSVNDLENVYLYDIDDLKELSQQHLSDRLAESEKARSIVDEEVKKFSQW
                     LRQLDKNPLISNIYEKVEEIRSRELKKILQKNKNTDDETMKNMDLLTKSIVNKLVHPH
                     VTMIKENGSPAILELMKKLFRLEDDDEQDVDSWHKG"
     gene            2957..3853
                     /gene="hemC"
                     /locus_tag="PK125_12320"
     CDS             2957..3853
                     /gene="hemC"
                     /locus_tag="PK125_12320"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: protein motif:HMM:TIGR00212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HPB38930.1"
                     /translation="MNRTWIVGTRGSKLALKQTEIVVNLLQQLYPDDTFHVKTIKTTG
                     DTVWDKPLHLIGGKGLFVKEIEEELLAGSIDFAVHSMKDMPTELDERLTIGAILKRED
                     PRDVFLSLKYKSLADIPGGGRIGTNSLRRQSQILFHKSNVSVIPIRGNVDTRIRKMES
                     EGLDGIILAYAGVIRMGFGNQIREILPFEIMVPPSGQGAIGIETRNEQDLLSMLGPIN
                     DEISYREVSIERNLQMRIGGGCNTPLGINATINKQTLILNMFFGRENGEIIMKDKQEG
                     NIEDADKMVTRASEKLALCRYS"
     gene            complement(3913..4182)
                     /locus_tag="PK125_12325"
     CDS             complement(3913..4182)
                     /locus_tag="PK125_12325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016535194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HPB38931.1"
                     /translation="MTKAEIIGKMAAGAKISKAAAGKALDAFIDGVKASLKKEERVTL
                     VGFGSFSISKRKARKGRNPKTGKEIKIAARKVPKFTPGKAFKDAV"
     gene            complement(4320..4691)
                     /locus_tag="PK125_12330"
     CDS             complement(4320..4691)
                     /locus_tag="PK125_12330"
                     /inference="COORDINATES: protein motif:HMM:NF013234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannose isomerase type II C-terminal
                     cupin domain"
                     /protein_id="HPB38932.1"
                     /translation="MVKEAKTLILEEDHRPWGYYQVISDEPDLKLKKIYVYPGKRLSL
                     QRHQRRTEHWYILQGKAIFTLDSKEIYLEAGMSVDIPRGSFHRIMATGEENLIFIEVQ
                     TGDYFGEDDIERVEDDYGRVP"
     gene            4795..5250
                     /locus_tag="PK125_12335"
     CDS             4795..5250
                     /locus_tag="PK125_12335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB38933.1"
                     /translation="MVASIWNIKSELNYYSTMIQCREIPPKADLLARTWLQTCREIEE
                     PEHVFIMDVREDGSLGCEDLWVFSKHYAMRVKNFIENEEVEIFGITKPISYMKLKASD
                     YDFKDFDARSKLSLTVQINPGFEVTIKASQGNCPILETIIKYLCTTNNY"
BASE COUNT         1612 a         1035 c         1095 g         1512 t
ORIGIN      
        1 acagttgaag tactgtgtga aggcacggac caagatgtac aactcatggt tgactggtgc
       61 gcgaaggggc cggaagggtc ttttgtcagc aagacggctg ttacacagga aaaatatact
      121 ggtgaattcg aaacattcca aattgtctat taaaagcaat atctattatc caatattttt
      181 agatataaca aaaaagcctt gcctggtcgt cgggggtggc agagttgccg aaagaaaggt
      241 tagcgttttg ctcaaattta acgggataat tagattagta agcccgcaaa tatcgaatac
      301 cttgaaaagg tatggtgata aaggaaggat tcacatatct ctcaaagagt atggtgaaga
      361 agacctggac ggtgttgcac tcgtgttcgc tgcaaccaac gttgagtcac tgaacgaaag
      421 aatatataac gatgcagtga aaaagaacat tcctgcaaat gttgttgaca acccttcact
      481 gtgcgatttc atcgttccct ctattgtcaa aaaagaccct ataattattg ccatatccac
      541 gtcaggcatg cttcctttcc tttctaaacg attgagaaag gatatggcag agggtataac
      601 cagggattat gtacggtatg caaacattgt aggacgattg agaaaactgc taatcatgga
      661 aataaaggac aaaaaactaa ggcagagctt aatgaaggaa attatgggaa cagatgtgag
      721 cgtgataaac aatatgggat tcaaaaaggc aaaggaaacc ttttttgggt cagaaaaata
      781 atgtttattc tttttctcat tgccctctgt ttgtatctgg tatcaacaat attctatctc
      841 cttttcctcg ctatcgacaa aaagggtata gaggcgttag gacatttttc acttctcagt
      901 ggatttattg ttcattttat ctccacatgc tttcgttatt ttcaggctgg ctacacgccc
      961 ataacgaatc ttcatgaatc cttatcgttt ctttccctct gtatggccgg tttctttttg
     1021 tttcttaaaa ggtcctacaa aatatctatt ctaggatcta taattgttcc ttttttgtca
     1081 ctcgtgttta tctgggccct atggttaccc agcgatatta aaccattggc accaattctg
     1141 caaagttact ggcttccagt gcacactata tttgccttca tcggaaattc aatttttttt
     1201 atcagcttct tcgtgtccat tctttacctg tctctggaga gagggattaa aagaaaaaaa
     1261 ttttcgccca tctccacccg gtttccttcc ctggaggtac ttgattcgat taattatcga
     1321 tgtatgtcat acggttttcc ttttctgaca atcgggataa tcacaggttc attatgggca
     1381 ggcatggcct ggggttcata ctggagttgg gacccgaagg aaacatggtc ccttataacc
     1441 tggattgtct atgccattct catccacaac agactcacta ttggctggag gggtcgcaaa
     1501 acagcctata tgatgattgc gggttttttt tccattctat ttacgtttct cggaataaat
     1561 tttcttgtag gaggtctcca ttcttatctc tagggctttt tcattgagcc aaagaaaaaa
     1621 caacacgatg ctgacaaata atcaagataa tgttctctgt ttcggagtct gctccttatt
     1681 acttgccatt tatgggagtc tggtttgcct atagcagtat ttggacttaa tcacaacact
     1741 gcaccaatcg aagtgagaga gagacttttc gtgtcggagg aaacaattcc tcgccttgta
     1801 cgagaactcc aggagagcgg cgtagatgaa aatattgttc tttcaacctg caacaggaca
     1861 gaattctact tctcatgcaa gaacctcgat tcagcccttg ataaattcta tcaagcactt
     1921 tccagacatt ttagtattga aaaggagtgg ctggaaaaat acacctattt gctcacagac
     1981 gaagacactt accgacacct tttccttgtt gcttccgggc ttgactcaat ggttgttggt
     2041 gagcctcaaa tcctgggtca ggtgaaggac gcgtatagac ttgccacgga ttgcaataca
     2101 agcggttttt ttctcgacaa ggtgtttcat aaaaccttca atgttgcaaa aagaataagg
     2161 acagaaacaa aagtcggata taacccggta tcaatcagtg ccatggctat tgaacttgcg
     2221 aagaagattt tcggtgaatt gaaggataaa aaaacccttg ttattggcgc gggagaaatg
     2281 tgtgagattg ccctaagata ttttaaaaaa gaaggtgtag gagatatcta catcacgaac
     2341 cgcacattcc acaatgccca gaaactggct gatgagacta ccggcatccc tcgcccgttt
     2401 aatgatcttc aggatcttct tgagaaagta gatatgattc tctcttcaac cggttcagag
     2461 agacctctaa ttagcaggga acttgtcagt gtggtgatga aaaaacgaaa gcaaaaacct
     2521 ttgtttttca tagacattgc agtcccgaga gatatcgaac catctgtcaa tgatctcgag
     2581 aacgtctacc tttacgatat tgatgatctt aaggagctgt cgcaacagca tctctctgat
     2641 cgacttgcag aatcagaaaa ggcccgctca atcgttgatg aagaggtaaa aaagttttct
     2701 caatggttaa ggcaactgga taaaaatcct ttgatatcaa acatatatga gaaagtggaa
     2761 gaaattcgaa gcagagaatt aaaaaaaatt ttacaaaaaa acaaaaacac tgatgacgaa
     2821 acaatgaaaa acatggattt gttaacaaaa agtattgtga ataaacttgt ccacccccat
     2881 gttaccatga taaaggagaa tggatcacct gcaatactgg aattgatgaa aaaactattc
     2941 agattagagg atgacgatga acaggacgtg gatagttggc acaaggggta gcaagcttgc
     3001 attaaaacag acggaaattg tagtaaacct gcttcagcag ttgtatcctg atgacacatt
     3061 ccacgtcaaa acaattaaaa ctacgggtga cacggtctgg gacaaacccc tgcaccttat
     3121 aggtggaaaa ggacttttcg ttaaagagat tgaagaagag ttattggcag gcagcataga
     3181 cttcgcagtg cacagcatga aagacatgcc aactgaactt gatgaaagac tcaccatcgg
     3241 ggctatcctg aaaagagaag acccgcgtga tgtatttctc tcgttaaaat ataaaagcct
     3301 tgctgatatt ccaggtggtg gtagaatagg taccaacagt ttgaggaggc agtctcaaat
     3361 cctgtttcat aaaagcaatg tcagtgtgat tcccattcgg gggaatgttg acacaagaat
     3421 cagaaaaatg gagtcagagg gcttggatgg gatcatatta gcatatgccg gtgtgatacg
     3481 gatggggttt ggcaaccaga tacgggagat tttgcctttt gaaatcatgg ttcccccatc
     3541 aggacaaggt gctataggaa ttgaaacaag aaacgagcag gatttgcttt ccatgcttgg
     3601 gcccattaac gatgagataa gctatagaga agtgtcaatt gagcggaatc ttcaaatgag
     3661 gattggggga ggatgtaaca ccccactcgg tatcaacgca actatcaaca aacaaaccct
     3721 cattcttaat atgttttttg gcagagaaaa tggagaaata attatgaagg acaaacagga
     3781 aggtaatatt gaggatgctg acaaaatggt taccagggct tcagaaaagc tggctttgtg
     3841 ccggtattca taaaaaaatg cctctcaaaa gatgagaggc atttacataa aaccgtatat
     3901 gggaaagaat cgttaaacag cgtctttaaa agcttttcca ggtgtgaatt ttggcacttt
     3961 gcgtgcagca atcttgattt ctttgccagt tttcgggttt ctcccttttc tcgcttttct
     4021 tttggagatg gagaaggatc caaatccaac aagagtaacc ctctcttcct ttttcaaaga
     4081 agccttcaca ccatcaataa aagcatctaa tgctttacct gcggctgcct ttgaaatctt
     4141 tgcacctgct gccatctttc ctattatctc ggcttttgtc atacccaccc ctccttataa
     4201 cgtttgattt ttcgtggttc tcataatgac cacccttgtg aatagtttat acagcttatc
     4261 acaaaaaata tgcaatactt ttttactatt tttattaaaa aaggtggttg gccgaaagcc
     4321 taaggaaccc tcccatagtc atcttccaca cgttcaatgt catcttctcc aaagtagtcc
     4381 ccggtttgca cttctataaa gatcaaattt tcttctccag tggccatgat gcggtggaag
     4441 gatcctcttg ggatatcaac ggacataccg gcttcaagat aaatctcttt tgaatccagg
     4501 gtaaaaattg cctttccctg aagaatatac caatgctctg ttctgcgctg gtggcgctgc
     4561 aagctgagtc tcttcccagg gtaaacatat atttttttta acttaagatc cggttcatct
     4621 gaaatgacct gataataacc ccacggacgg tggtcttcct ccaggataag agttttggct
     4681 tctttaacca tgagactatt ttgaaactgg tacacttctt tgtcaagaag tatttaacag
     4741 tcaaatagat aagctaaagt aaaattacga caaatccgat aaacctgtgt aatcatggta
     4801 gccagtatct ggaatatcaa aagtgagctt aactattact ccacaatgat ccagtgcagg
     4861 gagatacccc caaaggctga tcttcttgcc cgcacatggc tgcagacctg ccgggagatt
     4921 gaagaaccag agcatgtttt tatcatggat gttcgcgaag atggatcact tgggtgtgaa
     4981 gatctttggg tattttccaa acactatgca atgagagtta aaaatttcat tgaaaacgaa
     5041 gaagttgaaa tttttggcat tacaaaaccg ataagctata tgaagcttaa agccagcgat
     5101 tatgatttta aagactttga cgcacgctca aaattatctc ttacagtaca gattaaccca
     5161 ggttttgagg taacaatcaa agcctcccag ggtaattgtc ctatcctaga aactatcatt
     5221 aagtatcttt gtacaacaaa caattactag aatt
//
LOCUS       DAOOMC010000156         5217 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdus sp. isolate AMR_MDS_2685
            NODE_19064_length_5217_cov_5.665052, whole genome shotgun sequence.
ACCESSION   DAOOMC010000156 DAOOMC010000000
VERSION     DAOOMC010000156.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864760
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdus sp. (wastewater metagenome)
  ORGANISM  Syntrophorhabdus sp.
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae; Syntrophorhabdus.
REFERENCE   1  (bases 1 to 5217)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5217)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,729
            Genes (coding)                    :: 2,708
            CDSs (with protein)               :: 2,708
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 7 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5217
                     /organism="Syntrophorhabdus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19064_length_5217_cov_5.665052"
                     /isolate="AMR_MDS_2685"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2699756"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(79..663)
                     /locus_tag="PK125_12340"
     CDS             complement(79..663)
                     /locus_tag="PK125_12340"
                     /inference="COORDINATES: protein motif:HMM:NF015944.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HPB38934.1"
                     /translation="MKRFLVYALGIFVIMSLSGCGYNTMQINEEAIKAAWGDVEATYQ
                     RRNDLIPNLVEVVKSYAKHEKETLQAVTEARGKVGSIQAGADIVNDPKAFSQFQEAQA
                     SIGNALSRLLVVVEQYPDLKANQNFRDLQVQLEGTENRINVARTRYNKAVQEFNTSIR
                     IFPNSVTNSILLHLKPKEAFKAEAGAEKAPKVKF"
     gene            859..1263
                     /locus_tag="PK125_12345"
     CDS             859..1263
                     /locus_tag="PK125_12345"
                     /inference="COORDINATES: protein motif:HMM:NF014664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HPB38935.1"
                     /translation="MDENTLKKRTQETAKKLWTGGIKLDPPYLMWKEFDRDLANDLSM
                     FITGNLYSRTVLTLPERQMIACAILAALGAADELRLHVNAALNVGCDPKKLAETFFQL
                     APYGGMPLVNKALEVFKEVLKGRGEWESFHTE"
     gene            complement(1276..1686)
                     /locus_tag="PK125_12350"
     CDS             complement(1276..1686)
                     /locus_tag="PK125_12350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB38936.1"
                     /translation="MDNMKQCPYCAETIRNEARVCRYCFTDFQEIEKEKEGQFIRVRV
                     KTGDKTYAGDLFVPDYMSRVSDVVNDKKPFIILVNSVEETKAVDIKVGFIAINKNMVQ
                     SIRLSDEKQTEKNQEFVTRMTRFDDDVQRRIGRL"
     gene            complement(1829..4030)
                     /locus_tag="PK125_12355"
     CDS             complement(1829..4030)
                     /locus_tag="PK125_12355"
                     /inference="COORDINATES: protein motif:HMM:TIGR01448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HPB38937.1"
                     /translation="MLKSKKELVEIKGQVERITYANEENGYVIAKVRVPGRQDLVTVT
                     GNLFSVTPGELLRLTGLWNQHPKYGEQFRVYGYESVLPATVKGIERYLGSGMIKGIGP
                     VMARRLVSSFAEKTLDVIENSIEKILEVPGIGEKRVEMIRHAWEEQKEIRDVMIFLQG
                     QGVSPAYAVRIYRHYGNDAINVVKENPYRLAMDIFGIGFITADKIAEKLGIPKDSPVR
                     AQAGVLYVLHQLSDDGHIYYPYDSLIKKCSNVLQVEESGITYAVESIANQKKAIIEKL
                     GTLNTELRTSETRAVYLTPLHIAELGIVNRLKALFSHPKQMRLINIDESIVWSQKTLR
                     MTLSERQVEAVKASVHAKAMVITGGPGTGKTTIINAIIKIYSRMGQKVLLAAPTGRAA
                     KRMTEATGHEAKTLHRLLEYSPGGASFKKNESNPLEADLIIIDEVSMVDAVLMYHLLK
                     AVSMRATIILVGDVDQLPSVGPGNILKDIIDSEYLPTIRLKEIFRQSKESMIIVNAHR
                     INSGHMPTTFYDSKHKKDFYFFNIDDPEKVLEEIVSLCRDKINLRFGYDPMNDIQVLT
                     PMHKGTVGVTNLNTVLQSALNAVGDELVRGGRIYRVGDKVIQLRNNYDKDVFNGDIGR
                     IVAIDKEIQEVTINFDGKKVHYEFQELDEISLAYAISVHKSQGSEYPVVIIPILTQHF
                     VLLQRNLLYTGITRGKKLVILIGTWKALAIAIKNNTPQNRYTMLRERLKNR"
     gene            4174..5151
                     /locus_tag="PK125_12360"
     CDS             4174..5151
                     /locus_tag="PK125_12360"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HPB38938.1"
                     /translation="MDLSILVIDDEDGIRRSLTRILREDGYSVSVASRGEEALELILN
                     NPTKYDIVICDLLMPGIGGIATIEEINRLNSEITKIILTGYGTLESSIKAIEAGVDGF
                     ITKPFENKELTWKVRECYLKRKTKQFISDDIYKELLKEPNHLLPRLSFITILFSDIRG
                     FTQLSSQVPPDELASLLNNDYFHPMCDIIIKHKGMIDKYIGDSIMALFGAPVANESHA
                     ENAVNCAIEMVKRIKDNNGRFGMGIGISTGRVITGIFGSVSKKEYTALGMPVNLAARL
                     QKLAGPGDIIISDITKSRLNGLTPFQRAGTITFTTLSGPTTYFKWICDG"
BASE COUNT         1397 a         1221 c         1022 g         1577 t
ORIGIN      
        1 aaattctatt acttttcttt ttgatactgc agtggtgtct ctgaaccccg aaatccaaac
       61 tcataagcag ccttaagcct agaacttcac ctttggtgct ttttctgcgc ctgcctcagc
      121 tttgaaagcc tccttgggct tcaggtggag gagaatgctg tttgtcacac tgttcggaaa
      181 aatcctaata gaagtgttaa actcctgtac tgccttatta taccttgttc ttgccacatt
      241 aatcctgttt tcagtacctt ccaactgaac ctgaagatct ctgaagttct ggtttgcctt
      301 aaggtccggg tactgctcaa caacaacaag cagtctggat aaggcgttgc ctattgacgc
      361 ctgcgcctcc tgaaactggc tgaatgcttt ggggtcattt actatgtcag cacctgcctg
      421 tatactcccc accttccccc gtgcctctgt tactgcctgg agcgtttctt tctcatgttt
      481 tgcataggac ttcacaacct ccacaaggtt ggggataagg tcatttcttc tttgataagt
      541 agcctcaaca tcaccccatg ctgccttaat tgcctcttca ttaatctgca ttgtattgta
      601 accacaacct gatagactca ttattacaaa tattccaagt gcatagacca aaaacctttt
      661 cattcaaccc tccaaacaaa atcgtttttc agattccaaa acagtataga caaatactat
      721 catctcatca acctgaaata aaggttatag atcaagcgat ttcactgttt gacctttaat
      781 caatccatat ctaatttatt gacgacagag aaagatgcat gatacaaatt cctgaaccat
      841 tatacaagga ggatgattat ggatgaaaat acattgaaga aaagaacgca ggaaacagca
      901 aaaaagttgt ggaccggtgg tattaaactg gacccgccct atcttatgtg gaaggaattt
      961 gacagagacc ttgccaatga tctttccatg ttcattacag gcaatctata ctctcgaaca
     1021 gtcttaaccc tacctgaaag gcagatgata gcatgtgcta tactcgcagc attgggcgct
     1081 gcagatgagt tgcggctgca tgtgaatgcg gcacttaatg ttggttgtga tcctaaaaag
     1141 cttgccgaga ccttttttca gttagcacct tacggaggaa tgcctttagt caataaagcc
     1201 cttgaagtct tcaaagaggt gctgaaaggc cggggtgaat gggaaagctt tcatacagaa
     1261 taacgagtaa tctgtctaca aacgtccgat gcgcctctga acgtcatcgt caaagcgtgt
     1321 catccgggtg acaaactcct ggttcttttc ggtctgtttt tcatcgctaa gacgtattga
     1381 ttgcaccata ttcttgttta tggctatgaa gccgactttg atatcaacag ccttcgtctc
     1441 ttccacgcta ttcacaagaa taataaaggg cttcttatcg ttgacaacat cacttacgcg
     1501 gctcatatag tcaggcacaa ataaatctcc ggcataggtc ttatctcctg tcttgaccct
     1561 gacccttatg aactggcctt ctttttcctt ctcaatctct tgaaagtctg taaagcaata
     1621 cctgcaaaca cgcgcttcat tccttattgt ttctgcgcaa taaggacatt gtttcatgtt
     1681 atccatatat cctcctcatc cccacatttt ataaattcta tctcacttga tttcatcaag
     1741 ttgatatgtt tatcggacat tttcttgaga acaaaagtcc cccgatgctt tgcagtagat
     1801 tttttatttt ctttttgaaa agagggtatt accgattttt aagcctttcc ctgagcatgg
     1861 tatagcgatt ttgaggggta ttgtttttaa tggcaatggc aagtgccttc caggttccaa
     1921 tcaatattac aagcttcttt cctcgtgtaa tccctgtgta aaggaggttt ctctgaagaa
     1981 gtacaaaatg ttgcgtcaaa ataggtatga taaccacagg atattcactt ccctgggatt
     2041 tatgtactga tatggcatag gccaggctta tctcgtcaag ttcctgaaat tcgtaatgaa
     2101 ctttctttcc atcgaaatta atggttactt cctgaatttc tttgtcaata gcaacaatcc
     2161 ttccgatatc gccattgaaa acatccttgt catagttgtt tctcagctga atcaccttgt
     2221 cgcccaccct gtaaatcctt cctccccgaa ctaattcatc acccacagcg ttcaacgctg
     2281 attggaggac agtatttagg ttagtaactc caacagtacc tttatgcatt ggggttaaca
     2341 cttggatatc attcattgga tcatacccaa acctcaaatt gatcttatct ctgcaaaggc
     2401 ttacaatctc ctccaacacc ttttcggggt catctatatt aaaaaaatag aaatcttttt
     2461 tatgttttga gtcatagaag gttgtcggca tgtggccact atttattctg tgggcattta
     2521 caatgatcat gctctcctta gattgtcgga atatttcttt cagacggatt gtgggaagat
     2581 actctgaatc aataatgtct ttgagaatat tgccaggccc cacggatggc agttgatcaa
     2641 cgtcccctac gaggatgata gttgctctca ttgacactgc tttcaggaga tgatacatga
     2701 gaactgcgtc caccatggac acctcatcaa taataataag gtcagcctca agagggtttg
     2761 actcgttttt tttgaatgaa gctcctccag ggctatactc aagaaggcgg tggagagtct
     2821 ttgcttcatg gcctgttgcc tcggtcatcc tctttgccgc ccttccagtg ggggcggcaa
     2881 gaagcacctt ctggcccatc ctactgtaga tctttatgat tgcatttatt attgttgttt
     2941 ttcccgtacc cggtcccccg gtaattacca tggcctttgc atgaaccgaa gctttcaccg
     3001 cttcaacctg cctctcggag agtgtcatcc tcaaggtctt ttgcgaccac acgattgatt
     3061 catctatgtt tatcaatctc atctgcttag ggtgactaaa aagggctttt agcctgttca
     3121 ctatcccaag ctcagctata tgaagggggg taagataaac agctcgtgtt tcgctagttc
     3181 tgagttcggt attcagggtt ccgagctttt caatgatggc ttttttttgg tttgcgatgc
     3241 tttctaccgc ataagttatg ccagactctt ccacctgcaa aacattagaa cactttttga
     3301 tcaacgagtc gtaggggtaa tagatgtggc catcatcgga aagctggtga agcacgtaga
     3361 gaactcccgc ttgagcacgc accggcgagt ccttggggat gccaagcttt tctgctatct
     3421 tgtcggcagt gataaagcct atcccgaaga tatccatggc aagcctgtac gggttttcct
     3481 ttaccacatt gattgcatca ttaccataat gtctgtagat tctgacagca tatgcagggc
     3541 taaccccctg tccctggaga aaaatcatca cgtcccgtat ctccttctgc tcctcccagg
     3601 catgcctgat catctccacc ctcttctctc ctatgccagg tacctccaag attttttcta
     3661 tactattttc aataacatca agagtcttct cggcaaatga agatacgaga cgtcttgcca
     3721 taaccgggcc tatccccttg atcatccctg agccaagata tcgctctatc cctttcactg
     3781 ttgcaggaag tactgactca tagccatata ctctaaactg ttccccgtac tttgggtgct
     3841 gattccatag gccagtgaga cgaagcaact cacccggagt gacagagaac agatttcctg
     3901 ttacagtgac caggtcctgt ctaccgggca ctcttacttt tgcaattaca taaccatttt
     3961 cctcattagc gtaagtaatt ctttccacct ggccttttat ctctacgagt tctttcttgc
     4021 tcttaagcat gtacttatag tagcccactc gataacttaa aaaaagtata attttcctat
     4081 tctctgctgc tcaactccaa ctggttgtta agtctatttt tcattgtcat tttttcatcc
     4141 ttgtactatg attaaaaggt aggcttgcta tttatggatt taagtatcct tgtaatagat
     4201 gatgaggatg gtataagaag gtcccttacc aggatacttc gggaagatgg atactccgtt
     4261 tcggttgcgt ccaggggcga agaagctctc gagctcattc tcaacaatcc gaccaaatac
     4321 gatattgtaa tctgtgacct cctcatgccc ggcataggtg ggattgcaac catagaagag
     4381 atcaacagac tcaatagcga aataacaaaa attattctta ccgggtatgg tacattggaa
     4441 tcatcaatca aggccattga ggcaggtgtt gatgggttca tcacaaaacc attcgaaaac
     4501 aaggaactta cgtggaaagt aagagagtgt tacctaaagc gaaaaacgaa gcaatttata
     4561 tcagacgata tatacaagga actactcaaa gagcccaacc atcttctacc gcgtttatca
     4621 ttcataacaa tcctcttttc tgatatacgc ggctttaccc agctttcatc ccaagttcct
     4681 ccagacgagc ttgcgtcgct tctcaataat gactacttcc accccatgtg cgacatcatt
     4741 atcaaacata agggaatgat cgataagtac attggagaca gtatcatggc actctttgga
     4801 gcacctgtag ccaatgaatc tcatgctgaa aatgcagtta actgtgctat tgagatggtg
     4861 aaacgcataa aggataacaa tggaaggttc ggcatgggaa tcggcataag caccggtcgg
     4921 gtaataacag gcatattcgg gtctgtaagc aagaaagaat atactgccct cggcatgccg
     4981 gtcaacctgg cagcgcgttt acaaaaactg gcaggccccg gggatatcat tatttcggac
     5041 attacaaaat cgagactcaa tgggttaacc ccctttcaga gggcaggcac aatcactttt
     5101 acaactttgt cagggccaac tacatacttt aaatggatct gtgatggtta gaatcaacga
     5161 cattgttgag gaagttctga aatacaaccc ccaggctgat gtggagttgc ttcagaa
//
LOCUS       DAOOMC010000157         5114 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdus sp. isolate AMR_MDS_2685
            NODE_19614_length_5114_cov_4.958292, whole genome shotgun sequence.
ACCESSION   DAOOMC010000157 DAOOMC010000000
VERSION     DAOOMC010000157.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864760
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdus sp. (wastewater metagenome)
  ORGANISM  Syntrophorhabdus sp.
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae; Syntrophorhabdus.
REFERENCE   1  (bases 1 to 5114)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5114)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,729
            Genes (coding)                    :: 2,708
            CDSs (with protein)               :: 2,708
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 7 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5114
                     /organism="Syntrophorhabdus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19614_length_5114_cov_4.958292"
                     /isolate="AMR_MDS_2685"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2699756"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            184..708
                     /locus_tag="PK125_12365"
     CDS             184..708
                     /locus_tag="PK125_12365"
                     /inference="COORDINATES: protein motif:HMM:NF025652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4372 domain-containing protein"
                     /protein_id="HPB38939.1"
                     /translation="MDKVYNIFAELLKLCPRYQFEKATERYKGDRYVKTFTAWQHYIT
                     MLYSQITQKDSLRDIETGLGTQSSRWYHLGFKGVCRSTLSDANTKRDYRIFEDMFYHL
                     LARCRDLTPKHRFRFRNPLYTIDATTIDLCLSVFPWAKFRKAKGAIKMHCLYDHSGAL
                     PSFQTGTDGMRSQR"
     gene            964..1338
                     /locus_tag="PK125_12370"
     CDS             964..1338
                     /locus_tag="PK125_12370"
                     /inference="COORDINATES: protein motif:HMM:NF013752.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HPB38940.1"
                     /translation="MRKDKKTLTFLTNNFRLAATTIAQIYKSRWQIELFFKWIKQNLK
                     IKSFLGTNKNAVLTQIWVAMCYYLLLTSIKYQTKYGYSLLHLSRIIRETLFDRKSLID
                     ILTLHPERLSAARNEMLQGALF"
     gene            complement(1451..2194)
                     /locus_tag="PK125_12375"
     CDS             complement(1451..2194)
                     /locus_tag="PK125_12375"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HPB38941.1"
                     /translation="MGFSLYLKKRLGNFLLDVSWEMDNELLVLFGPSGAGKSLTLQMI
                     AGLIEPDEGFIYSGKRILFDRAKSIHIPPQQRSLGYVFQDRVAFPHMTVRENIAYGLR
                     NGRKADREHKVNEMINLFHLEMIARKYPSEISGGQKQRVTLARALIGRPDALLLDEPF
                     SALDTVLRTGMRALLRYIRQHFNTPIILVTHDIQEAYSMADKVVVYSSGRISQIGTPS
                     EIFQDSSNLDADLHNLPPLTAPAPELVFP"
     gene            complement(2196..2861)
                     /gene="modB"
                     /locus_tag="PK125_12380"
     CDS             complement(2196..2861)
                     /gene="modB"
                     /locus_tag="PK125_12380"
                     /inference="COORDINATES: protein motif:HMM:TIGR02141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HPB38942.1"
                     /translation="MDSTILFSLRLSLQVAFVATLAVIVVGVYVAYLLARKDFRGKEF
                     VDILFTLPLVLPPTVTGYYLIILFGRNGFLGRYIYDLTGWSIMFTWYAAALASFVVAL
                     PLMVKTSRAAFESVDKSLIEASFTLGHTELKTALKVIIPIAKRGIIAGAILSFARALG
                     EFGATLMVAGNIPGKTSTMPLTIYSLANSGEWSRANILVIFFTCMSGVFLYVANKLSK
                     RLA"
     gene            complement(3084..3392)
                     /locus_tag="PK125_12385"
     CDS             complement(3084..3392)
                     /locus_tag="PK125_12385"
                     /inference="COORDINATES: protein motif:HMM:NF014664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HPB38943.1"
                     /translation="MKDVHKIFTTFKKDFPDICKRHEALGKDIHERSGPLPEKTRWLI
                     KVAISGASGHHLALETHIVRSREAGATEDEIKQVLLLLIQTTGFPTFMEAYTVYKNLG
                     "
     gene            complement(3897..4670)
                     /gene="modA"
                     /locus_tag="PK125_12390"
     CDS             complement(3897..4670)
                     /gene="modA"
                     /locus_tag="PK125_12390"
                     /inference="COORDINATES: protein motif:HMM:NF024921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_function: GO:0030973 - molybdate ion binding [Evidence
                     IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HPB38944.1"
                     /translation="MKLLVVSACMVFALFSWAFSAGPNEITVSAAASLKNAFEDMGKA
                     FEFQNKGIKVYLNFASSGDLKKQIEAGAPVDVFASAAQREMDDLERKGGLTAGTRRNF
                     AANEMVLARPSASTVQLNSFNDLARKEIKKIGIGNPPSVPAGMYAEQVLKHFGVWETI
                     KDKLIFGESVRQVLDYVARGEVDAGIVFSTDALARSKDVVVVMKAPEGSHVAVVYPMA
                     VVKNTKNEKVARGFVDFVMSDNGRRILEKYGFKASAASK"
BASE COUNT         1480 a         1185 c         1095 g         1354 t
ORIGIN      
        1 tcaattccgc ttccaccctg gaagcatgag gaagatagtt tcaggcaacg caatattcaa
       61 taaaaatatt cataacctct catcagtact gtccggtaaa acttcataaa agtatggcct
      121 gttctttatt cttgtaggat aatgaagtca ccaaaaccca aaccctgcaa gaaggaacag
      181 accatggata aagtatataa catattcgca gagttgttaa aactgtgtcc gagatatcaa
      241 tttgaaaagg caactgagcg ctataagggc gaccggtacg tgaagacctt tacggcatgg
      301 cagcactata tcaccatgct ctactcccag atcacacaga aggacagctt acgagacatt
      361 gagaccggac tcggtacgca gtcaagtcgc tggtatcatc tggggttcaa aggggtgtgc
      421 agaagtaccc tctctgacgc gaatacaaaa agagactatc ggatcttcga ggatatgttc
      481 tatcaccttc tggcacggtg cagggatctg actcccaagc acaggttccg gttcagaaac
      541 cccctctata cgatcgatgc gaccaccatt gatctgtgcc tgtcggtgtt tccctgggca
      601 aagttcagga aggccaaagg ggccatcaag atgcactgcc tctatgacca ctcgggggca
      661 ttgccgtcct ttcagaccgg caccgacggc atgaggtcac agcggtaaag gaaacctcct
      721 ttcccctgtc accggacagc atcgtgtctg tcgataaggc atatatcgat tacacatggc
      781 tgtattcact gaacgtaaag aggatatggt ttgtcaccag agcaaagtcc aacatcgatt
      841 atgccgttac cggacagcat ccggtaaaag gccaaggggt catacgtgat gagcggatat
      901 cccttacagg accccttacc aaagacaaat atccccggga tttgagactt atacggttct
      961 ttgatgagga aagacaaaaa aactcttacg tttttaacga acaacttcag gcttgcagcc
     1021 acaacgatag cccagatata caaatcccga tggcagattg aactcttctt caaatggatc
     1081 aagcagaacc tgaagatcaa atcattcctc ggaaccaaca agaatgcagt cctgacacaa
     1141 atatgggtgg ccatgtgcta ctatttactt ctgacctcca tcaaatacca gacgaaatac
     1201 ggatattcgt tgttgcacct gagcaggatt atccgtgaaa cgctttttga ccgaaaaagc
     1261 cttattgata ttcttaccct gcatcctgag cgtctgtctg ctgcgagaaa tgagatgttg
     1321 cagggggctt tattttaacc ggacagtaat gatgatgtat tataggtggg aataaacaaa
     1381 gacttgcttg ttcctccttg acaccagaaa taaacttgaa acaaacaatc agaaaattaa
     1441 ccggaaggtt ttatggaaaa actagttctg gagctggcgc agttagtgga ggcagattat
     1501 gcagatctgc atctagattt gaggaatcct ggaatatttc actaggtgtc cctatctggc
     1561 taattctccc gcttgaatag accacaacct tgtctgccat tgaatatgct tcttggatgt
     1621 catgtgttac cagtatgatg ggggtattga agtgctgccg tatgtaacgc aacaacgctc
     1681 tcatcccagt gcggagaact gtgtcaagtg cagaaaatgg ctcgtccagg agaagagcgt
     1741 caggtcgtcc gataagtgca cgtgcaagag ttaccctctg cttttgaccg cctgaaatct
     1801 cgctgggata cttacgagct atcatttcaa gatggaacag attgatcatt tcgtttacct
     1861 tgtgttccct atcagctttt cgaccgttcc tcagtccgta tgcaatattt tctctcactg
     1921 tcatgtgtgg gaaagcaact ctgtcctgaa atacatatcc gagagagcgc tgctggggag
     1981 gaatatgtat tgattttgct ctatcgaaga ggatcctttt gccggaatat atgaaccctt
     2041 catccggttc aataagacct gcgatcatct ggagcgtcaa tgatttgcct gccccggatg
     2101 gaccgaacaa gacaagcaac tcattatcca tttcccatga gacgtctagc aagaagttac
     2161 ccaatctttt tttcagatag agcgagaatc ccatgctacg ctaacctctt gctgagtttg
     2221 ttagcaacat agaggaacac tccggacata cacgtaaaga atattaccag tatattcgcc
     2281 ctcgaccatt ccccgctatt tgcgagagag tagatggtca atggcattgt gcttgtcttg
     2341 ccgggtatgt tgcctgcaac catcagcgta gcaccgaact cacccagtgc tctggcaaaa
     2401 gaaaggatgg ccccggctat gatgcctctt tttgcaatag gaataataac tttgagcgct
     2461 gttttaagtt cggtatggcc gagcgtaaaa gaggcttcga taaggctttt gtcgacagac
     2521 tcaaaagcag cacggcttgt cttgaccatg agagggagag caaccacaaa agaagcaagt
     2581 gcggcagcgt accacgtaaa cataatgctc caaccggtga ggtcgtaaat atagcgcccc
     2641 aaaaatccat tccgtccgaa aaggatgatc aggtaataac cggttacggt tggtggcaac
     2701 accagcggta gggtaaagag gatgtcaaca aattccttgc ccctgaaatc cttcctggct
     2761 aacaaataag ccacatacac acccacgact attacggcca gtgtcgcaac aaaggctacc
     2821 tgcaatgata gccttaatga aaagagtatt gtactatcca tgaagtacac caagtatttc
     2881 ggagtaattt gccaacgggg taggaccaac ccctgaaaat aaatatcatt aagtaaaagc
     2941 gaacattgaa accatggttt cttttccaga catcctggtg accgttattt accatagaaa
     3001 ataaccattg tcaagatttg atgccgtttc atctgcggtg tgacaaacca ttgtcagtat
     3061 tacaccaacc aactatcgtg cggttaaccg aggttcttgt atacggtata agcctccata
     3121 aacgtgggaa atcctgtggt ctggataagc aggagaagga cttgtttaat ctcgtcttcc
     3181 gtcgctcctg cttctcggga tctaacaata tgcgtctcaa gggccaagtg gtgaccgctt
     3241 gcaccggata tagcaacctt gatcagccag cgggtctttt ctggcagtgg cccggacctc
     3301 tcatggatgt ctttacccag tgcctcatgc ctcttgcaga tatctgggaa atccttcttg
     3361 aatgttgtaa aaatcttgtg aacgtctttc atgggagacc tccttatgat tgttttttct
     3421 tgggaaagcc atagctaaga cgcaaagtct catgacaagt atatatttga ctgagacaat
     3481 gtcaatgatt aaatcttttc aggaaagctt ttcgctgttt gctatgagat tgttcattca
     3541 agcttgaaaa tgagcagcgt tttacaacgc atccctataa ttgtggacgc atgtcggctt
     3601 ttgcttagat ctattatttt tcagttcaag gaacaggata agcccgtttg actaactgga
     3661 agaattacat tttctaagcg acgagcagta tggatgataa gcccagatgc cacatgaccg
     3721 cattaggatt agcgacaaat agcgccctct gacgaaagac tcaaggagag gacatcgtcg
     3781 atagataaaa ggaaatatta ggttttagcc gcccaagcac ctggtgaggc ataggcggct
     3841 aacgtaaagg agttaaatag aagcatgttg agaggtgagc aatattggat acatcgctat
     3901 ttagaagcag ccgaggcctt aaagccatat ttttctaata ttcttctacc attatcggac
     3961 atcacgaaat ctacaaaacc ccttgcaacc ttttcattct ttgtgttttt gacaaccgcc
     4021 atcggataga cgactgccac atgactccct tcaggagcct tcatcacgac cacaacgtct
     4081 ttggatctcg ccagtgcatc agttgaaaac actattccag catcaacttc acctcgagct
     4141 acgtagtcca gcacctgcct gacagattct ccaaagatca gtttatcctt tattgtttcc
     4201 cagacgccga agtgtttcaa tacctgctct gcatacatcc ctgcgggtac cgaaggaggg
     4261 ttcccaatgc ctatcttctt gatctccttt ctggccaaat cattaaatga gttcaactga
     4321 acggttgagg ccgaaggcct cgccagaacc atctcgttgg cagcaaaatt acgccgtgtc
     4381 cctgccgtca aaccaccctt tcgttcaaga tcgtccattt cgcgctgggc cgcagatgca
     4441 aatacatcaa caggtgcgcc tgcttctatc tgttttttaa gatcacctga gctggcaaag
     4501 ttcaagtata cttttatccc cttgttctga aattcaaaag cctttcccat gtcctcaaaa
     4561 gcattcttca gacttgctgc tgctgacaca gtaatctcat tgggcccagc actaaaagcc
     4621 catgaaaaca aagcaaaaac catacaagca gacactacga gtaatttcat tcgtgcaaac
     4681 actttcccta tttacagcct ccttatatac caatggtaat aacgttgttc gttatatacc
     4741 atcgggaaga atgcttgtca agaaatttgt ataaaaatgc ttgacaggta tttattcata
     4801 actttatatc ggtaaataac gggctgttgc ccaaaccctc aagattaact gcatgagcga
     4861 tgattttctt acgagaaatt atccataaat gtatacaata ttgttggatt aacatggctg
     4921 tatatgatag aataacctat tgtaactaca aggagatacg acgtatgatg ggttatcata
     4981 aatatcccca ggaaaacatg tttttataca atatctatct ggaaaacagg atccgggaag
     5041 accacccctt acatagaatc agagagatta ttgacttcga ttttatctat aaagaggcgg
     5101 aagatacatg cggt
//
LOCUS       DAOOMC010000158         5097 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdus sp. isolate AMR_MDS_2685
            NODE_19707_length_5097_cov_4.952598, whole genome shotgun sequence.
ACCESSION   DAOOMC010000158 DAOOMC010000000
VERSION     DAOOMC010000158.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864760
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdus sp. (wastewater metagenome)
  ORGANISM  Syntrophorhabdus sp.
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae; Syntrophorhabdus.
REFERENCE   1  (bases 1 to 5097)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5097)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,729
            Genes (coding)                    :: 2,708
            CDSs (with protein)               :: 2,708
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 7 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5097
                     /organism="Syntrophorhabdus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19707_length_5097_cov_4.952598"
                     /isolate="AMR_MDS_2685"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2699756"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..215
                     /locus_tag="PK125_12395"
     CDS             <1..215
                     /locus_tag="PK125_12395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB38945.1"
                     /translation="KWKSISILPDELDMVGVVIRRYLLPFFCAPVQSLFLQLFSQFHF
                     HPGYPRHNHCYSAHCIHNILWIALQV"
     gene            complement(656..1504)
                     /locus_tag="PK125_12400"
     CDS             complement(656..1504)
                     /locus_tag="PK125_12400"
                     /inference="COORDINATES: protein motif:HMM:NF024587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidylate kinase-like family protein"
                     /protein_id="HPB38946.1"
                     /translation="MAILTISRQYGSGGREVGRAIAKAMDYDYIDRQRIVEDMRAEGK
                     LWKDKAEYFDENYPDIWERNDWAFRGFVALNQSHFLQHAVKGNAVIMGRGGNFLLKQF
                     PFVLKVRIIAPVERRISNIMEREDTNSENARYLIDKVDSEMAKAVYLIYGRNMDDPKE
                     YDMVFDTSQRSQDDIVTILRSALLEKDKYDTPATRQALKLRALAEKIKATILSDPRFM
                     ISTLDVDPKEEGMEKYGLRVRGIVHRREDLGLIEEIVRQMADNLPVEFQISFMAFPRF
                     GRLRFK"
     gene            complement(1515..2846)
                     /locus_tag="PK125_12405"
     CDS             complement(1515..2846)
                     /locus_tag="PK125_12405"
                     /inference="COORDINATES: protein motif:HMM:NF014135.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HPB38947.1"
                     /translation="MGYMEWAASIIFLASIILVITGWIDSVLAALLGILFMIYFGVMS
                     DIDAFKIVDWNVIAILLSIWIISGYFGRSGVPDFLSAIMLKLSKGNVALFVTLIGMLA
                     GYVSIVIDNVVVVLMFAPVVFHACRRFNFEAFTPVLFVGLCSNFMGTAMLLGDLPPQM
                     LHSVTGIEFSGFIWFMGKPSSFFILNISYIITCAAFYCIFSRKYKNISMDVASMEEHP
                     LDHIKDKPFALISCGIFVLTIIAMALRPIFGYTLGFIAMWGAICIILFFELFKSRFTL
                     EIPDVEHVLGELDWRAIFFYVSLFALVGGLEHAGVIKLVSNALTPLIQKSLIVGSTAL
                     YWITAPIVGIVEHDAYILALLYVIRDLGHAHGINPWPLYWMLLWAGTLGSNLTIAGAP
                     ALFVAKSMGEKEDQRQVGLKEFLGITTPYVVISLIFCYIPAMLIWVLPYAK"
     gene            complement(2956..4095)
                     /locus_tag="PK125_12410"
     CDS             complement(2956..4095)
                     /locus_tag="PK125_12410"
                     /inference="COORDINATES: protein motif:HMM:NF015910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium ion-translocating decarboxylase subunit
                     beta"
                     /protein_id="HPB38948.1"
                     /translation="MVDGIIGGLSGLIAGVLNFHWSGMVMIVIGAILIYLGVKKDFEP
                     LLLVPIGFGCILVNIPLGGLMEEHGFLRVLYDMGVLNELYPLLIFVGIGAMTDFRPVL
                     ENPYTFLLGAAGQFGIFLTLLIALALGFPYNEAVTIGIIGACDGPTVIYVASKYAREL
                     LGPCSVAAYSYMSLVPLLQPPIMRMLTTKKERQTIMKTHKKSVSDTTALLFPIVITIV
                     GGLIAPMGLPLLATIMLGNFMKESGAVARLTKASENEIANIVTLFLGLSIGATMAGPV
                     FVKPQTILILALGLLAICLDTVFGVLFGKILYLVTGGKVNPLIGAAGISAFPMSARVV
                     QKEGQKWNKKSYLLMHAMGANAGGQVGSVMAAAVMLSVLKGLGVI"
     gene            complement(4114..4248)
                     /locus_tag="PK125_12415"
     CDS             complement(4114..4248)
                     /locus_tag="PK125_12415"
                     /inference="COORDINATES: protein motif:HMM:NF040909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OadG family protein"
                     /protein_id="HPB38949.1"
                     /translation="MIDKWTFGLTMLVVGMGGTIATLIVFSLIMSVMKKIFPYRKEDK
                     "
     gene            complement(4535..4870)
                     /locus_tag="PK125_12420"
     CDS             complement(4535..4870)
                     /locus_tag="PK125_12420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB38950.1"
                     /translation="MDPEKKIGIILILIGIIIPLIALPFVSGFSKEKGFYDNFYNAGI
                     VLGKDSVDSTPGEPLIKGKKPIIQLKLTWAMLRPGKIPFRFILVPTVILIFMGIIRID
                     RARKRKPDE"
     gene            4916..>5097
                     /locus_tag="PK125_12425"
     CDS             4916..>5097
                     /locus_tag="PK125_12425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB38951.1"
                     /translation="MGSFAKLNPLFKIVKKPVGAQISTQGHYLCAVSVPYTDSQDTPE
                     TYYPPRLIENPVHRKPS"
BASE COUNT         1534 a         1219 c         1025 g         1319 t
ORIGIN      
        1 gaaaatggaa gtccatatca atacttccag atgaactgga tatggtcggg gttgttatac
       61 gcagatacct tcttcccttc ttctgtgcac ctgtccagag cctgtttctt caactctttt
      121 ctcaatttca ttttcatcca ggataccccc ggcacaacca ctgttacagc gcccattgta
      181 tccataatat actgtggatt gcattgcagg tatgaagaaa taactgcacg tatgggccag
      241 tgatgattat ttcaacagta acttaccccg gggtcatggt accaggacga atgaagatca
      301 ggtatttatc catcagcatt gctgaaggtt aaggttccgg actgccattg cgatgataac
      361 cgcgttatcc ccagtaagca caagttcgat ggggataacc atatagatac tgcttataaa
      421 gcctcacgtg aagttaaaat ggccaaagct tccaggattc ttgttcaccg cacaggaaaa
      481 gctttgaatc atgaataagg agtcgccata cctatagcag gctgatatca gcacacggtc
      541 aatcagggct attctgaatt tatgggtgcg gtgagtctaa aaattcaggt tcggaaaagt
      601 ggggtggcaa ctgagatttt tcaattttga cttaatcctg catcagagca cccagctact
      661 tgaagcgcaa cctgccaaaa cgtggaaatg ccatgaaact gatttgaaat tcaacaggca
      721 gattgtctgc catttgtcta acaatttctt ctatcagccc aagatcttct ctcctgtgca
      781 caatacctcg aacacgcaat ccatattttt ccataccctc ttctttaggg tccacatcca
      841 gagtggagat cataaaacga gggtcagaca ggatagttgc tttaattttt tcagcaagag
      901 cacgtagctt cagggcctga cgagtcgctg gagtgtcgta tttgtctttt tcaaggagag
      961 cactcctcag aatggtaaca atgtcatcct ggcttcgctg agaggtgtca aaaaccatgt
     1021 catattcttt tggatcatcc atattcctgc catagatgag ataaacagct tttgccatct
     1081 cactgtctac cttatcgatg agatatcttg cgttctcaga gttcgtatct tcacgttcca
     1141 tgatatttga aatccgtctt tcaacaggtg caataatcct gactttcagt acaaatggga
     1201 attgtttgag gaggaaattc cctccccgtc ccatgatcac tgcgttgccc ttcacagcat
     1261 gctggaggaa atggctctgg tttaatgcca cgaatcctct gaaagcccaa tcatttctct
     1321 cccagatgtc cgggtagttc tcatcaaaat attcagcctt gtctttccaa agttttcctt
     1381 cagcacgcat atcttctaca atcctctggc gatctatgta gtcataatcc atggctttag
     1441 caatagcacg gccgacttct cgtccaccgc ttccatattg tctcgatatt gtaagaatag
     1501 ccataccaca ccccctattt tgcgtatggt aatacccaga taagcattgc cggaatgtaa
     1561 cagaagataa gtgaaatgac tacataagga gtggtaattc cgaggaactc cttcaggcca
     1621 acctgccgtt gatcctcttt ctctcccatg ctttttgcta caaagagggc aggagcaccg
     1681 gcaatggtga ggttgctccc aagggtgcct gcccagagaa gcatccagta aagtggccag
     1741 ggattgatgc catgggcatg gccaagatcc cttataacgt acagcagggc aaggatatat
     1801 gcatcgtgtt caacaatgcc cacaatgggc gctgttatcc agtagagtgc ggtgctgccc
     1861 acgatcaggc tcttctgaat cagcggggta agagcgtttg agacaagctt gataacgcct
     1921 gcatgctcaa gacctcctac aagggcaaaa agggaaacat agaaaaagat agctctccag
     1981 tcaagttcgc caagcacatg ttccacatcg ggaatttcaa gggtaaacct gcttttgaac
     2041 aactcaaaaa agagaatgat gcatatagcc ccccacatgg caataaaccc cagggtgtac
     2101 ccgaaaatag gccgcaatgc catggcaatg atggtaagca cgaaaattcc gcatgaaatt
     2161 aatgcaaatg gtttgtcttt gatatggtcg agagggtgtt cttccattga ggcaacatcc
     2221 atggaaatat tcttgtactt cctggaaaag atacagtaaa aagctgcgca ggtaattata
     2281 taagatatat tcaggatgaa aaaggatgaa ggcttgccca taaaccagat aaacccgctg
     2341 aactctatac ctgtaacgct gtggagcatc tgaggcggca aatctccgag aagcatggcc
     2401 gttcccataa aatttgaaca gaggcctaca aacaaaacag gggtgaatgc ttcaaaatta
     2461 aatcgcctgc atgcatgaaa aacaaccgga gcaaacatta ggaccaccac aacattgtcg
     2521 atcactattg agacataacc agcgagcata ccgataagtg ttacaaaaag tgccacatta
     2581 cccttcgata gcttaagcat tatggcagat aggaaatccg gcacccctga tcttccgaaa
     2641 tagcctgata tgatccagat actgagtaat atggctataa cattccagtc tacaatctta
     2701 aaggcatcaa tgtcactcat gacaccaaag tagatcatga aaagaatgcc caataatgca
     2761 gctaatacgc tatcgatcca gcctgtgatg acaaggatga tgctggcgag aaaaatgatg
     2821 cttgcagccc actccatata tcccatgatg attcccctcc caaatatttt ttatccggac
     2881 cttaggtata gtttgggcgg gaagaatccc gcccaaacta taatgagttt gtatacaatt
     2941 ttcaatgcaa ataaatcaaa ttactcctaa gcctttgagc acggagagca taactgctgc
     3001 agccataaca gaaccgacct ggccgccagc gttggcaccc atggcatgca tgagtagata
     3061 gctcttcttg ttccacttct gtccctcttt ctggacaacc ctggctgaca ttgggaatgc
     3121 agaaatacct gctgcaccga taagagggtt tacctttccc cctgtcacca gatagagtat
     3181 cttgccaaac agaaccccaa aaaccgtatc aagacaaata gccaaaaggc caagggcaag
     3241 tatgaggatg gtctgaggtt ttacaaatac cggtcctgcc atggtggcgc caattgatag
     3301 cccgagaaaa agggtgacaa tgtttgcgat ttcgttttcc gatgcttttg taagtctggc
     3361 cactgctcct gactctttca tgaaattgcc aagcataatc gtcgcaagga gcggcagacc
     3421 cataggagcg ataagtccgc caacaattgt gattacaatg ggaaaaagca acgcagtggt
     3481 atctgaaacc gatttcttgt gtgttttcat aattgtctga cgttctttct ttgttgtgag
     3541 catcctcata atcggcggct ggagcaaggg taccagggac atatatgaat acgctgcaac
     3601 cgaacatggg cccaacaatt ctctggcata cttggaggcc acgtagataa ctgtaggacc
     3661 gtcacaggca ccgataattc caatggttac agcctcatta taaggaaaac caagggcaag
     3721 ggcgataaga agggtcagga atataccgaa ctgaccggct gccccaagaa gaaacgtgta
     3781 cgggttctca agaacaggtc gaaaatccgt catggctcct atgccgacaa agataagcag
     3841 aggatataat tcattgagaa cacccatatc atagagcacc ctgagaaaac cgtgctcttc
     3901 cataaggcct cccagaggaa tattcaccaa aatacatcca aaaccaatag ggacaaggag
     3961 taacggttca aagtccttct ttaccccgag ataaatcagg atagccccta taacaatcat
     4021 caccatacct gaccagtgaa aatttagaac gcctgcgatt aaccctgata atccgccaat
     4081 tattccatca accatttcgc ctgcctcctt atcctattta tcttctttcc tgtaggggaa
     4141 aatctttttc ataacactca taatcagact gaagacgatg agcgtggcaa ttgttcctcc
     4201 cataccaact acaagcatcg ttaatccaaa ggtccattta tctatcatgc taaccacctc
     4261 ctaagatgat tataaactac tggttgatca aaagcttcgg ctcaattttt gaagtttgag
     4321 acaaatattt attacactcg tcgaaacatg tgtcaagtga aataattaac aatattctta
     4381 ttccccagta tatcaaacag atagcgttcc ccatacccac aactgcccta tcttttaacc
     4441 catatacagc acaacaggca atcaggaaat ccctgatatt gaccttcaaa aaaccttact
     4501 tcaatacaaa atgcaacaca ccaaaaaaag acctttattc gtccggcttt ctcttcctcg
     4561 cccggtcaat tcttattatg cccataaaaa tcagaatcac cgttggaacc aggatgaatc
     4621 tgaaaggtat ctttcctggc ctgagcatcg cccacgtgag cttaagctgt ataatgggct
     4681 tctttccttt gataaggggt tcacccgggg tactgtccac agagtctttt cccaatacaa
     4741 tcccggcatt gtaaaaatta tcatagaaac ccttttcctt cgaaaaacct gaaacaaagg
     4801 gcagtgcaat cagagggatg ataatcccga taagaataag tattatccca attttttttt
     4861 caggatccat tttgatctac actataccat aattaactga aaaaataata gacccatggg
     4921 gtcattcgct aaacttaatc cactgtttaa aatagtgaaa aaacctgttg gggctcagat
     4981 atcgacacag ggacattact tgtgtgccgt cagtgtgccg tacactgatt cacaggacac
     5041 tccggaaaca tactatccac cacgccttat tgaaaatcct gtccacagaa aaccttc
//
LOCUS       DAOOMC010000159         5072 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdus sp. isolate AMR_MDS_2685
            NODE_19836_length_5072_cov_4.564680, whole genome shotgun sequence.
ACCESSION   DAOOMC010000159 DAOOMC010000000
VERSION     DAOOMC010000159.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864760
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdus sp. (wastewater metagenome)
  ORGANISM  Syntrophorhabdus sp.
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae; Syntrophorhabdus.
REFERENCE   1  (bases 1 to 5072)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5072)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,729
            Genes (coding)                    :: 2,708
            CDSs (with protein)               :: 2,708
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 7 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5072
                     /organism="Syntrophorhabdus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19836_length_5072_cov_4.564680"
                     /isolate="AMR_MDS_2685"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2699756"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2059)
                     /gene="feoB"
                     /locus_tag="PK125_12430"
     CDS             complement(<1..2059)
                     /gene="feoB"
                     /locus_tag="PK125_12430"
                     /inference="COORDINATES: protein motif:HMM:TIGR00437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HPB38952.1"
                     /translation="MNSELFSVISLNEGEEGVVNLVSGGESLTSRLAGMGIVPGTRIK
                     ILRNTGSLIIVLATDTRIALGRGQAEKILVVKVKDTHDLQEKATRRKLLVALAGQPNV
                     GKSTVFNVLTGLSQHVGNWPGKTVEKKEGIHVSGDTEIRIVDLPGTYSLSAFSEEERV
                     ARDFVIHEHPDIIVILVNASALERSLYLLSEVLLLDTPVIVAVNMIDVAADQGTKVDI
                     EALANSLKLPVVPMIAAKNKGIKELVSEIVRVADNKVNYDVKLPTVSADHMEIYLKLT
                     ELLKGYIPVPYTERWLVTKLMEGDPEITKLLEDIVPIDTWTQIQLLLIKHEDALRAVV
                     GGRYDWIEEIARSSVSRFKRGQILMTDRIDHILTRPIIGIPVLLGILAIIFMVTFKVG
                     LPIQELLGTLAGKAGQWLDEALTQTPWWVRGLVVNGILGGVGSVLTFVPLLVIFFATM
                     AFLESVGYMARAAFVMDRFMHLIGLHGKSFLPMCLGFGCNVPSVLGARIVESKRARLL
                     TIFLTPFVPCTGRLAVLTFISGAIFYDKALMVTWFLVTLNIVSLGVAGMLISKLFLKG
                     EPVPFIMELPLYHKPDLKTIGIVIWNRTMAFVKKAGTVILIFSVVLWAISNIPGGKVE
                     HSLLGWVGHFIEPIGRPLGLDWKLMVALLSSIVAKENAVATLGVLYNVGEQGLQGVLP
                     SAIN"
     gene            complement(2144..2713)
                     /locus_tag="PK125_12435"
     CDS             complement(2144..2713)
                     /locus_tag="PK125_12435"
                     /inference="COORDINATES: protein motif:HMM:NF015325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HPB38953.1"
                     /translation="MNIIAFNGSPRAGGNTELLLGETLKAIDPDLHRIQLFRLNQVDI
                     KPCQNCGGCDDTGICVIKDAMDDIYEAIRLADRIVLASPIFFFGLSAQTKIMIDRCQA
                     FWCEKYLLKKTYTSEPHGRKGLLLLVGGMKSDTGIRCGEATARAFFRSISVPEHETLG
                     FLNVDAKGSIVDHPTAFQQGFEAGARLVK"
     gene            complement(2801..3295)
                     /locus_tag="PK125_12440"
     CDS             complement(2801..3295)
                     /locus_tag="PK125_12440"
                     /inference="COORDINATES: protein motif:HMM:NF014912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HPB38954.1"
                     /translation="MSKTEDGLKEAFAGESQANRKYLAFAKKAEEEGYKQVAKLFRAA
                     AEAETVHAHNHLRELKGIKSTKENLESAISGESYEFLTMYPAMIADAQAEGNKSAERS
                     FHIANEVEKVHHNLYKKALEELGKNKETDYYICKVCGYTAEDEAPDICPVCGAKKVAF
                     YKVD"
     gene            complement(3386..3517)
                     /locus_tag="PK125_12445"
     CDS             complement(3386..3517)
                     /locus_tag="PK125_12445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubredoxin"
                     /protein_id="HPB38955.1"
                     /translation="MWRCTVCGYEYDEAVEGTPFEKLDDDWHCPICNAPKDAFEKVK"
     gene            complement(3530..4750)
                     /locus_tag="PK125_12450"
     CDS             complement(3530..4750)
                     /locus_tag="PK125_12450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="FprA family A-type flavoprotein"
                     /protein_id="HPB38956.1"
                     /translation="MKPIEIKPDIYWVGAIDWAVRDFHGYITPHGTTYNNYLIKDEQV
                     TIVDCVKHDFVSTSLANIKAVVDPTKIVNLIINHIEPDHVSGISEIMSVMPQAVIYIS
                     ERGKKGLERYADTTGWTIKIVKSGDSLNIGKYTLSFLETPMLHWPDSMVTYIKEAKLL
                     ISQDAFGQHLATAQRFDDEFLSCHSLFELEDAVKDYYANILMPFGQLIKAKLAEIVKL
                     GLEIDMIAPDHGIIWRENPGRILQMYSDMADGKADLAVSIIYDTMWHSTEIMIFPIME
                     GIKDEGVPCNVIKLRATPTSVAVKEFWKSRATLIGTPTINNVMFPSVAEFLYHLGGLR
                     PKNRLMGAFGSFGWGGGGVKEATESIKKTGLEIVEPGLQIMYKPTDEDKAKCYEFGKD
                     FARKVKEYHKKFEK"
BASE COUNT         1410 a         1200 c          939 g         1523 t
ORIGIN      
        1 gatttatagc agatggcaac accccctgaa gtccctgttc gccaacatta tacaggacac
       61 cgagagtggc aacagcattt tcttttgcca cgatacttga aagaagggca accatcagtt
      121 tccagtcaag ccctaatggc cgaccaattg gctcaataaa atgccctacc cagcccagca
      181 ggctatgctc taccttacca ccgggaatgt tagaaatagc ccacaaaaca actgaaaata
      241 ttaaaattac tgtccctgcc ttctttacaa aggccatggt cctgttccat atcacgattc
      301 caatcgtttt taagtcaggt ttatggtaca aaggcaactc catgataaac ggaacaggtt
      361 ctcctttcaa aaataattta ctgatcaaca ttcctgcaac acccagtgaa acaatgttca
      421 atgttactaa aaaccacgta accattaaag ccttgtcata gaatattgct ccagaaataa
      481 atgtgagtac agccaatcgc cctgtgcaag gcacaaatgg tgtcaaaaaa attgttaaaa
      541 gtctggccct ttttgactcg actattcgtg ctcctaaaac tgatggcaca ttgcatccga
      601 agccgagaca catgggaaga aagctcttcc cgtgaagacc gatgaggtgc ataaacctat
      661 ccatcacaaa tgcagctcgc gccatatacc ctacactttc aagaaaagcc attgttgcaa
      721 aaaaaatgac aagaagaggc acaaaggtca gaactgaacc aacgccgcca aggataccat
      781 tcacaacgag gcctcttacc caccacgggg tctgcgttaa tgcttcatcc agccattgac
      841 ctgcttttcc agccagtgtt ccaagaagtt cttgtatggg aagacccact ttaaacgtaa
      901 ccataaatat aatggctaaa atacccagaa gaacagggat accgataatt ggccgtgtca
      961 agatgtgatc gatacgatct gtcatcaata tctgtcctct tttgaacctt gagacagagg
     1021 agcgggcaat ttcttctatc cagtcatacc ttcccccaac aaccgctctt aaagcatctt
     1081 cgtgtttgat cagcagtaac tgaatttggg tccacgtgtc aatcggcacg atatcttcta
     1141 ataattttgt gatttcaggg tcaccttcca taagttttgt tacaagccat ctctccgtgt
     1201 aaggaacagg tatatacccc ttaagaagtt ctgtcagctt cagatagatt tccatatgat
     1261 cggctgaaac tgtgggcagc ttcacatcgt aatttacttt gttgtcagca acccgaacaa
     1321 tttcagatac cagttcctta atgcccttgt tcttcgccgc aatcattggc acaacaggca
     1381 gctttagtga attggccaac gcctcgatgt cgactttggt gccttgatct gcagcaacgt
     1441 caatcatatt gacagcaaca atcaccgggg tatcgagcaa aagaacctct gaaagaaggt
     1501 aaaggcttct ttccagggct gaagcattga caagtataac aattatgtct gggtgttcgt
     1561 gaattacaaa atctcttgct actctttcct cttctgaaaa agcactaagg ctataagtcc
     1621 ctggtaaatc gacgattcgt atttcagtat cccctgatac atgaatccct tctttctttt
     1681 caacagtttt tccaggccaa tttccaacat gttgagacag gcctgttaac acattgaaaa
     1741 cagttgactt acccacattc ggctgaccag caagagcaac aagtagcttt cttcgtgttg
     1801 ccttttcctg gagatcatgg gtatctttca cctttacaac caggatcttt tctgcttggc
     1861 cacgtccaag ggcaattctc gtatctgtgg caagaacaat aataaggctg cctgtattgc
     1921 gcagtatctt tatccttgta ccaggcacta tccccatgcc tgccagcctg ctggttagtg
     1981 attctcctcc tgacacaagg ttaaccacac cttcttcacc ttcgttaagc gagataaccg
     2041 aaaagagttc actattcata tagagccttt ctagatatac ggcagagata agacacgaat
     2101 ttgtttatac tatggcaaac cgggtaatgt caatgaccct gtcttacttt acaagtctgg
     2161 ctccggcttc gaaaccctgc tggaacgcag ttggatgatc aacaatcgag cctttggcat
     2221 caacattcag aaaaccaagc gtttcgtgtt ccggtacact tatgctcctg aaaaaagccc
     2281 tggccgttgc ttcgccacac ctgatgcctg tatcgctctt catgcctccc acgagcaaca
     2341 aaagtccctt tctcccatgc ggttctgatg tatatgtttt tttaagaaga tatttctcac
     2401 accaaaaagc ctgacagcgg tcaatcatga tctttgtctg tgctgaaaga ccaaagaaaa
     2461 aaatcggcga ggcgaggaca attctatctg ccaaacgaat tgcctcatag atatcatcca
     2521 tggcatcttt gatgacacag atgccagtat catcacaccc accgcaattc tggcatggct
     2581 taatatccac ctgattaagc ctgaaaagct gaattctgtg aaggtctgga tcaattgcct
     2641 ttaaagtctc accgagaaga agttctgtgt tgcctcctgc acgtggacta ccattgaatg
     2701 cgataatatt caacgaatga acctgcttag ccttgattca attttccggg acccagtttt
     2761 gagattatcg cctcggaacc cgatgattca tgcatatctc ttaatctact ttgtagaatg
     2821 ccaccttttt tgcaccacag accgggcaga tatcaggggc ctcgtcctct gcggtgtacc
     2881 cgcaaacctt acaaatatag tagtctgttt ccttgttttt acctaactct tccagggctt
     2941 tcttgtatag attatggtgt accttttcta cttcattggc aatgtgaaaa ctgcgttctg
     3001 ctgatttgtt cccttctgcc tgtgcatccg caatcattgc aggatacatg gtgagaaatt
     3061 cataggattc accgctaatt gcagattcca gattttcttt tgtgcttttg atgcctttca
     3121 attctcttaa gtggttgtga gcgtgaactg tttcggcttc tgctgctgct ctgaacagtt
     3181 tagcaacctg tttatatcct tcttcttcag ctttttttgc aaaggcaaga tacttcctgt
     3241 tcgcctgtga ttctccagcg aatgcttctt tcaaaccatc ttctgttttc gacatgacaa
     3301 cacctccata tacaatatta attctgcgat cagcaaatta gcggttagct ttaaaaaaac
     3361 tgaatactct aatctaatag cccgattatt ttactttttc aaacgcatct tttggtgcat
     3421 tgcaaatggg gcagtgccag tcatcatcaa gcttttcaaa gggtgtacct tccactgcct
     3481 cgtcatattc ataaccacaa accgtacatc tccacataga ttttcctcct tatttttcaa
     3541 atttcttatg gtactctttt actttacgtg cgaaatcttt tccgaattcg taacatttag
     3601 ctttatcttc atctgtcggt ttatacatga tttgaaggcc tggttctact atttcaagcc
     3661 ctgttttttt gatagattca gttgcttctt tcacgcctcc acctccccaa ccaaagctac
     3721 cgaaagcacc catcaatctg ttttttggcc tcagaccccc taaatggtaa agaaattcag
     3781 caacagaagg aaacatgaca ttgttaattg taggtgttcc gatcagggtt gccctcgatt
     3841 tccaaaattc tttaactgca acactggtgg gagtagcacg caactttatg acattgcaag
     3901 gcacgccttc atctttaatg ccttccataa tgggaaagat cattatctcg gtgctgtgcc
     3961 acattgtatc gtatattatt gatacagcca gatcagcctt tccatcagcc atatctgagt
     4021 acatttgtag aatccttcct gggttctcgc gccaaataat gccgtgatca ggggcaatca
     4081 tatctatctc gaggcccaat ttaacaatct ctgcaagctt tgctttgata agttggccaa
     4141 acggcataag gatgtttgca taataatctt ttactgcatc ctcaagttca aaaagtgagt
     4201 gacagctaag aaattcatcg tcgaatcgct gtgcagtggc aaggtgctgg ccaaatgcat
     4261 cctgggaaat gagaagcttt gcctctttta tatacgtcac catagagtca ggccagtgaa
     4321 gcataggtgt ttcgaggaaa ctaagcgtat atttacctat atttaaactg tcccctgatt
     4381 tgactatctt tatggtccag cctgtggtat cagcgtatct ttcaagtcct ttctttcccc
     4441 tttcggaaat atagattacc gcttgaggca taacagacat tatttcactg attccactca
     4501 catggtcagg ctcgatgtgg tttataatta agttgactat ctttgtcgga tcaacaacag
     4561 ctttgatgtt agccaatgac gtggatacaa aatcatgttt tacgcaatct acgattgtaa
     4621 cctgttcatc tttgattaga taattgttat atgttgtacc atgaggagta atgtaaccgt
     4681 ggaaatcacg cacggcccag tcaattgctc ctacccagta aatatcaggt ttaatttcaa
     4741 tcggtttcat tatacctccc catttttaca ttcattaatt tcaagacaca catacttgac
     4801 aattttgttc aagaaaatgt gatttaatat aaaacaagga aatgggaata tcaaggcgtt
     4861 gattgacctt tcttgtatat catgataata aagtgttcat gtacacaaca agaatatact
     4921 accaggatac tgacgccgga ggagtggtat attttgctaa ttacttgaaa ttttttgaaa
     4981 agtcctggtt cgaatattta gtctctatag gcatttcact tcctgaatgg gaaaaggcag
     5041 gaacctacgt tctcattaag aatgcctttc tg
//
LOCUS       DAOOMC010000160         5021 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdus sp. isolate AMR_MDS_2685
            NODE_20103_length_5021_cov_6.717882, whole genome shotgun sequence.
ACCESSION   DAOOMC010000160 DAOOMC010000000
VERSION     DAOOMC010000160.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864760
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdus sp. (wastewater metagenome)
  ORGANISM  Syntrophorhabdus sp.
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae; Syntrophorhabdus.
REFERENCE   1  (bases 1 to 5021)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5021)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,729
            Genes (coding)                    :: 2,708
            CDSs (with protein)               :: 2,708
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 7 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5021
                     /organism="Syntrophorhabdus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20103_length_5021_cov_6.717882"
                     /isolate="AMR_MDS_2685"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2699756"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..259)
                     /locus_tag="PK125_12455"
     CDS             complement(<1..259)
                     /locus_tag="PK125_12455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB38957.1"
                     /translation="MEIIFIALKDSLALTLKLLCIILPLSISYEFLKHRQSSIEKKNF
                     AVFGISGKGFIPLVTGIIIGLTYGAGVIIYSLRTTNTTKKEA"
     gene            complement(241..702)
                     /locus_tag="PK125_12460"
     CDS             complement(241..702)
                     /locus_tag="PK125_12460"
                     /inference="COORDINATES: protein motif:HMM:NF019292.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside recognition domain-containing
                     protein"
                     /protein_id="HPB38958.1"
                     /translation="MLVQNTLLHMNTFYSTVKRGFLNGILITYKMVKVIVPCYIAIEF
                     IKHLGLIEVISAFFKPCMSIFGLPGESALGLMAGYFINLYAAIAVITPLQLSSKDMTT
                     IALMLGIAHSLPIETGVTRQTGVNAWLMLAARVVFSLFAGIVINLVWKLFS"
     gene            781..1704
                     /locus_tag="PK125_12465"
     CDS             781..1704
                     /locus_tag="PK125_12465"
                     /inference="COORDINATES: protein motif:HMM:TIGR03168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase family hexose kinase"
                     /protein_id="HPB38959.1"
                     /translation="MIYTITQSPVLNRTVEVEEIIYDDINEIVEAKNSVGGKGIDISR
                     VIKELGGQSVVLGFVGGYNGIEVEGKLVNEGIVCDFTRINDESQTKIIIYQRKKNLQT
                     LLSTPEPEIKPSEVSALHNKIKEVPGESYVVLGGSVPKDMDVNFFAQVITTLKKKNVR
                     VVLDADEEASARGLDAGPFLIKPNIHEFGRLINKNVRDLEDVFEEAKPFLKKVDYIVV
                     SAGARGVAGISREGSYHVIPPKIKVRSSLGAGDSLVAGIVFVLSKGGSFEESLVMGVA
                     CGTASTLNPGNILCSKEDIEIIKKDVMIKRF"
     gene            1793..1981
                     /locus_tag="PK125_12470"
     CDS             1793..1981
                     /locus_tag="PK125_12470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB38960.1"
                     /translation="MKKLVVLFIAITFFVSFSLISCGPSAEPPKPAPPKEEAKPAPEP
                     AKAEPEKAEPAKEEPAKK"
     gene            2048..2581
                     /locus_tag="PK125_12475"
     CDS             2048..2581
                     /locus_tag="PK125_12475"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HPB38961.1"
                     /translation="MKLKMFGLILVVFMFGAFLSGCGCFQEAAKGETAAPPPPPPKAA
                     PPEAKKEVPVAPAPAPAPAPAPVVVLKDINFDFDKYNIRPGDAEILKDNLGWFKANQG
                     KRVKIEGNCDERGSVEYNLVLGQKRADSTKNYLINLGVDGKLLDTISYGKERPICTEK
                     NEDCWAKNRRAHFAPLP"
     gene            2496..3710
                     /locus_tag="PK125_12480"
     CDS             2496..3710
                     /locus_tag="PK125_12480"
                     /inference="COORDINATES: protein motif:HMM:TIGR00238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KamA family radical SAM protein"
                     /protein_id="HPB38962.1"
                     /translation="MARSDQSARRRTKIAGRRIEGHTSHHYRKLSRKREGGSTAFPFV
                     STAEQLSQHIFLTEESKKDIQEVSEIFPLKIPVFYLNLMDRENPACPLRKQSVPSKDE
                     LLSTGHIDPLDEQRFLVTPSFIKKYPNRGVFLAASHCAMFCRFCNRRRFVGRSRHPMH
                     TWEESFDHMKRDGDLTEVIVSGGDPFTLPREDFSYMMRRLKNIERIRVIRISTRLPVV
                     CPEGVDKDHFKTLKKSSPIWIVIHINHPREVSPEFVDVIKKLSATGASIVSQTVLLRG
                     VNDCPHILLRLFELLVAQGVKPYYLFQFDEVKGAMHFKVRIQEGIHIMHFLRRHGSGL
                     AMPQYALDITGGYGKVPLDYNYVKRRKGTSLHLESPSGDEGIYIDNGKKGLCQDCGIC
                     GRKTAKVKKMRS"
     gene            complement(3767..4342)
                     /gene="pgsA"
                     /locus_tag="PK125_12485"
     CDS             complement(3767..4342)
                     /gene="pgsA"
                     /locus_tag="PK125_12485"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HPB38963.1"
                     /translation="MKVRDEAASLWTLPNRLSILRILFIPIIVLFIASQEDGLLLASC
                     LLFIIAGITDGLDGYLARKMSLTSRLGLYLDPIADKLLVTSVLITLTYYRQVPLWVTL
                     ILVGREFLINGLRAFYAMEGIAIYPSSFGKIKTALQLIGISCLLFNIAIERIDTIVHQ
                     IGLVTIYGALFFSIYSATNYLAALFKPQVSK"
     gene            complement(4332..4928)
                     /locus_tag="PK125_12490"
     CDS             complement(4332..4928)
                     /locus_tag="PK125_12490"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HPB38964.1"
                     /translation="MVFRLLILITMCIFPLQGMCGIYGYVDESGVFHFTNIKPANNKK
                     YRVIVPDSPKPALGFYAKTVNTDQYDSLIKQHSFNHGVDPTLVKAVMKAESNFNSQAV
                     SPKGAQGLMQLMPDTARHMSVYDPFDPDDNIRGGTKYLRLLDDTFQGNLELILAAYNA
                     GPNRVKEHNMNVPPFEETRNFIKRVKHFYNKLKNTHEG"
BASE COUNT         1550 a          916 c         1197 g         1358 t
ORIGIN      
        1 aggcctcttt cttggttgta tttgtggttc gtaaggaata gatgattaca cctgccccat
       61 aggttaggcc aatgatgatt cctgtgacga ggggaataaa cccttttcca gaaataccaa
      121 agacagcaaa attctttttc tcgattgaag attgcctgtg ttttaaaaat tcgtaagaga
      181 ttgacaatgg caatataata cagaggagtt ttaaggtaag cgccaatgaa tcttttaagg
      241 ctatgaaaat aatttccata ccagattgat tacaatgcct gcaaacaggc tgaacacgac
      301 tcgtgctgcg agcatgagcc aggcgtttac tcctgtctga cgggtcactc ctgtttcaat
      361 agggaggctg tgggctatgc cgagcataag tgctattgtg gtcatatctt ttgatgacaa
      421 ttgaagaggg gttatgaccg caatagctgc ataaagatta ataaagtagc ctgccattaa
      481 accgagggct gactcacctg gaaggccaaa gatactcatg catggtttga agaatgcgct
      541 aattacctcg atgagaccaa ggtgtttgat aaattctatg gcaatgtaac agggcacaat
      601 aaccttgacc atcttgtatg tgattaatat tccgtttaga aagcctcttt ttacagttga
      661 gtaaaaggta ttcatatgca aaagtgtatt ttgaactaac attgtgaacc acaaaaaaca
      721 atagataatt ttttctatag cgatttttca atatatttca tagaatacat ccagaataca
      781 atgatttata cgatcacaca aagccctgtg ctgaatagaa ctgttgaggt agaggaaatc
      841 atttatgacg atataaatga gattgtagaa gcgaaaaaca gtgtgggagg caagggtata
      901 gatatttcaa gggtaattaa agaacttggt ggacagagtg ttgtccttgg atttgttgga
      961 ggatataatg ggatagaagt cgagggcaaa ctggtaaatg aaggtattgt gtgtgatttt
     1021 acaagaatta atgatgaaag ccagacaaaa ataatcatct atcagagaaa aaagaacctt
     1081 cagacactcc tcagcacccc tgagcctgaa ataaaaccca gtgaggtttc ggccctgcac
     1141 aataaaatca aagaagtacc tggtgaaagt tatgttgtgc tgggcggatc agtgccaaag
     1201 gatatggatg tcaatttttt tgctcaggtc ataaccactc ttaaaaaaaa gaatgttagg
     1261 gtagtccttg atgcagacga agaagcgtct gcacggggcc tggatgccgg tcccttcctg
     1321 ataaaaccaa acattcacga atttggaagg cttattaata agaatgttag agatttggaa
     1381 gatgtttttg aagaagcaaa gccgttcctg aaaaaggttg attacattgt tgtctctgca
     1441 ggggcacggg gggttgcagg tatttcaagg gaaggcagtt accatgtaat accaccaaag
     1501 ataaaggtgc gcagttctct tggtgccgga gactccttgg tggcgggcat tgtttttgtt
     1561 ttgagtaagg ggggaagttt tgaggaatcc ctggtaatgg gtgtggcctg tggtacggca
     1621 tctacattga atcctggaaa tatattatgt tcaaaggaag acattgaaat cattaaaaaa
     1681 gatgttatga ttaaaagatt ttaatttact tattgaccat gatgtattat atatttccca
     1741 cagggaaagt tgggcaagag aattgattaa acacgaaagg aggtgagtgt agatgaagaa
     1801 gttagtggtg ttgttcatag ctattacatt ttttgtgagc ttttcactga ttagctgtgg
     1861 tccttcggca gagccgccaa aaccggcacc accaaaggag gaagctaaac cagcgccaga
     1921 gccagcgaag gcagagccag agaaggcaga gccagcaaaa gaagagccag cgaaaaagta
     1981 ggtactggat atcggggtat atccttgtca tgaggatata cccactgtcc aaaggaggga
     2041 gtggaatatg aaattgaaaa tgtttgggct tatattggta gtgtttatgt ttggagcttt
     2101 cttgagtgga tgcggatgtt ttcaggaggc agcgaaaggc gaaactgctg cgccgccgcc
     2161 acctccacca aaagcagctc cgcccgaagc caagaaggaa gttcctgttg caccagcccc
     2221 tgcaccagca ccagcaccag ccccggtcgt ggtgttaaaa gatatcaatt ttgattttga
     2281 taaatataac atcaggcctg gtgatgcgga aatactgaag gacaaccttg gttggttcaa
     2341 agcaaatcag ggaaaaaggg ttaaaataga aggaaactgc gacgagcggg gatctgtgga
     2401 gtataacctt gtccttggtc agaagagggc agactctaca aagaactacc ttatcaacct
     2461 cggtgttgat ggaaaacttc tggacaccat tagctatggc aaggagcgac caatctgcac
     2521 ggagaagaac gaagattgct gggcgaagaa tagaagggca cacttcgcac cattaccgta
     2581 aattgtcaag aaagagggag ggcgggagca ctgccttccc ttttgtttcc acagcagaac
     2641 aactctcgca acatatattt ctaactgaag aatcaaaaaa ggatattcag gaagtatcgg
     2701 aaatttttcc tctaaaaata ccggtattct atctcaacct gatggatcgg gaaaaccctg
     2761 catgtccttt aaggaaacaa tccgttcctt cgaaggatga acttctaagc actgggcata
     2821 ttgatcccct cgatgaacaa cgtttcctgg taacgccttc tttcattaaa aaatatccga
     2881 atcgaggtgt ttttctagca gcttctcact gtgctatgtt ttgcaggttc tgcaataggc
     2941 gaagatttgt aggccgaagc aggcatccaa tgcatacatg ggaagaaagt ttcgatcata
     3001 tgaaaagaga tggagatttg acagaggtta ttgtatcagg tggtgacccg tttaccctgc
     3061 cacgggagga tttctcatat atgatgagaa gactcaaaaa tattgaaagg attcgtgtaa
     3121 tccgtattag tacgaggtta cctgtagtat gcccggaggg agtagataaa gaccatttta
     3181 agacgttgaa gaaaagttca cccatttgga tagtgattca tattaatcat ccacgagagg
     3241 tttcccctga gtttgttgac gtaatcaaaa aactgagtgc gacaggagcc agtatagtaa
     3301 gccagacagt ccttttacgt ggagtgaatg attgccccca tatcctttta aggctttttg
     3361 agttgcttgt agcccagggt gtaaagccat actatctttt tcagtttgat gaggtaaaag
     3421 gggcaatgca tttcaaggtt aggatacaag agggaattca catcatgcat ttcttacgga
     3481 gacatggctc tggtttggca atgccccagt atgccctgga cataactggt ggttatggaa
     3541 aggtgccgct tgactataat tacgtgaaaa gaagaaaggg gacatcactt cacctggaaa
     3601 gtccatcagg cgatgaaggc atttacatag acaatggcaa aaagggcctg tgccaagatt
     3661 gtggtatctg tggacgcaaa acagcaaaag tcaaaaagat gagaagctaa aaggctgaga
     3721 acttgaggtt tcaaatcatt caattaattt acaaatcaaa gaattatcac tttgaaacct
     3781 gtggcttgaa gagggcagcg aggtagttgg ttgcagagta gatactgaaa aataatgcac
     3841 cataaatagt tacaagccca atctggtgga caatcgtatc aatacgctca attgcgatat
     3901 taaaaagcag gcaagatatc ccgataagct gaagtgcagt ctttattttt ccgaatgacg
     3961 aaggatagat tgctatccct tccatggcgt agaaagcacg cagcccattg atcaaaaatt
     4021 ctcttcctac cagaatcagc gttacccaga gcggcacttg acggtaatat gtcagggtaa
     4081 taagcacgga agtaacaagt agtttgtcgg cgatagggtc tagataaaga ccgagtctgg
     4141 atgttaggct catcttcctt gccaagtagc cgtcaagccc gtcagttatc cctgctatta
     4201 taaagagaag acatgaagca aggagcaatc catcttcttg agaggctata aaaagtacta
     4261 ttatggggat aaaaagtatt ctgagaatag aaaggcgatt aggcagcgtc cagagggatg
     4321 ctgcctcgtc tctaaccttc atgggtattt tttaacttat tataaaagtg ttttaccctc
     4381 ttgatgaagt tacgggtttc ttcgaagggg ggaacattca tgttatgttc tttgacccgg
     4441 tttgggcctg cattatatgc ggctagaata agttcaagat ttccctgaaa ggtgtcgtca
     4501 agaagcctca ggtattttgt gccgcccctg atattgtcgt ctggatcaaa agggtcatac
     4561 acgctcatat gccgggcagt atcaggcatg agctgcataa ggccctgagc accctttggg
     4621 gaaacagctt gagaattaaa atttgactct gctttcataa ctgctttcac aagtgtgggg
     4681 tcgacgccat gattaaagga gtgttgcttg ataagagaat catattggtc agtattgact
     4741 gtttttgcat agaacccaag ggctggcttg ggcgaatcag gcacaatgac tctatatttt
     4801 ttattgtttg caggcttgat attggtaaaa tgaaagacac cactttcatc aacgtagcca
     4861 taaataccac acatcccttg aagaggaaat atgcacattg ttatgagtat gagtagtcta
     4921 aaaaccattt tcctattttc ttaaactttt tcgtaattgt caagcctttt acaagcaata
     4981 cctttatcgc aaaatacttt caaagtttaa tattcgccta g
//
LOCUS       DAOOMC010000161         4974 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdus sp. isolate AMR_MDS_2685
            NODE_20376_length_4974_cov_5.619028, whole genome shotgun sequence.
ACCESSION   DAOOMC010000161 DAOOMC010000000
VERSION     DAOOMC010000161.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864760
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdus sp. (wastewater metagenome)
  ORGANISM  Syntrophorhabdus sp.
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae; Syntrophorhabdus.
REFERENCE   1  (bases 1 to 4974)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4974)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,729
            Genes (coding)                    :: 2,708
            CDSs (with protein)               :: 2,708
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 7 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4974
                     /organism="Syntrophorhabdus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20376_length_4974_cov_5.619028"
                     /isolate="AMR_MDS_2685"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2699756"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..434
                     /locus_tag="PK125_12495"
     CDS             <1..434
                     /locus_tag="PK125_12495"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HPB38965.1"
                     /translation="DVEAIKEGLRNNTIDIIATDHAPHEGTSKDVEFDIASFGISGFE
                     TAFALSLALVHEGVMTLPDLLRKFTVNPAKLLKLPYGQLAPGKVADIIIFNPDVEWKV
                     DRNSFLSRGKNSPFHGWVLKGKNLLTLVDGKIVYRDKSFKA"
     gene            complement(431..925)
                     /locus_tag="PK125_12500"
     CDS             complement(431..925)
                     /locus_tag="PK125_12500"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF039181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative metallopeptidase"
                     /protein_id="HPB38966.1"
                     /translation="MAIRYEDVTESVMEMFRKVKSQHFQELKNAKIRVLFDLKKRKSG
                     GRIVVGRILKANDLVKHLTKNANAEEGYDFIITLDKKCWDHVTDEDREKILRHELRHV
                     SFDIEADDNPYKLVNHSILDFYEEVEMNQNDPRWRERVATLTEDIYEQEKEARLEKKK
                     KARS"
     gene            complement(1119..2039)
                     /locus_tag="PK125_12505"
     CDS             complement(1119..2039)
                     /locus_tag="PK125_12505"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: protein motif:HMM:NF004160.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HPB38967.1"
                     /translation="MQVFESLDFEEKFANPILTIGNYDGLHLGHRKIIERVEIKAREN
                     KGTSMLMTFHPHPLTILKPDRFIGLITPLPVKRRLIEEAGIDVMFIIPFTDEFHLISP
                     ESFVENILVQKLGIKGLIVGYDFKFGKGGKGNVEYLAAKSTQYGFFFDIQEAITLDNE
                     KVGSNRIRRMIQEGDVKKAGLHLGRPYMIGGTVMAGDGRGRTIGFPTINLQTEFPLIP
                     GRGVYVSSVEIGGKRLPAVTNIGFNPTFDGQSLTIETYIMDFSQDLYNQKVALYFLDR
                     IRDEVKFSSVDELKDRIRKDVEIARAYFTI"
     gene            2090..2174
                     /locus_tag="PK125_12510"
     tRNA            2090..2174
                     /locus_tag="PK125_12510"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2124..2126,aa:Leu,seq:cag)
     gene            2321..3604
                     /locus_tag="PK125_12515"
     CDS             2321..3604
                     /locus_tag="PK125_12515"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HPB38968.1"
                     /translation="MREAVIVSCARTAIGQFGQSLKDIPVVQLGGIAIKEAIKRAGIR
                     PSKNKDKEFAPDIFGGKTDTDLEAKYYDFDPGLKEVIIDEVIMGNVLQGGLGQNSARQ
                     ASINGGVPKETAAYTLNKVCSSGLRAIIAGIHSVMVGDNDVVVAGGMENMSMAPFAVP
                     SARWGARMFDTKMIDLMVLDGVWEIFYGYHMGVTAENIAARYGISRVEQDTFGLISHQ
                     RARKAIKEGLFKDEIVPVIIPQKKGDPIIFDTDERPMDTTLEKMAKLAPAFKKDGSVT
                     AGNASGLNDCASAAVIMSREKANELGIKPLGKLVSWANGGVDPAYMGLGPVPAIRKAL
                     KKAGLTLDQMDVIELNEAFASQSIACIRELGVDTEKCNMFGGGVSLGHPIGCTGARLV
                     TTAFYAMQRMNKKYALISMCIGGGMGLAAIFEREA"
     gene            3608..4390
                     /locus_tag="PK125_12520"
     CDS             3608..4390
                     /locus_tag="PK125_12520"
                     /inference="COORDINATES: protein motif:HMM:NF012596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HPB38969.1"
                     /translation="MEYKNLLLEIKDGIAVVKINRPKALNALNSETLDELRHAGDAIN
                     ANKDVWVVIITGEGDKAFIAGADILEMKDMNPYQGMIFSQRGHEAFAAFENMNKPVIA
                     AVNGFALGGGFEVALMCDIIYASDKAKVGFPETTLGIFPGFGGTQRTAKLCGLAKAKE
                     LIFTGKMISAKEAYEIGLINKVVPHEELWKEVMALAEKIRSNGPISMGLAKALINKSL
                     FLDMESALMLEAKDFGLCFGTKDQKEGMTAFVEKRKATFTGE"
     gene            4411..>4974
                     /locus_tag="PK125_12525"
     CDS             4411..>4974
                     /locus_tag="PK125_12525"
                     /inference="COORDINATES: protein motif:HMM:NF013200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HPB38970.1"
                     /translation="MKIVACVKQVPDTEAEVKWDIPGGKLKRDAMDCVTNAFDEYAIE
                     EALLTRENHDGEIVAISMGPEKAMDVLRNCLALTVNEVKLCTDAAFEGSDTFATASVL
                     AAAIKKVGDVDIVYCGKQSTDGNTGVVGAALASILGWSQLTYVSKIRSIDGAGKKIVV
                     ERAIEGGTEVVEAKLPAVVSVLKGINEP"
BASE COUNT         1395 a         1086 c         1180 g         1313 t
ORIGIN      
        1 aagatgtgga agccataaaa gaaggcctga ggaataatac gatcgatatc atagccacag
       61 accatgcacc ccatgaaggc acctcaaagg atgtggaatt tgacattgca tccttcggta
      121 tctcaggctt tgaaacagca ttcgccttgt ctctcgctct tgtgcacgaa ggagtcatga
      181 ccctccctga cctgctgaga aaattcacag taaacccagc aaaactgctt aagcttccct
      241 acgggcaatt agcccccggc aaggttgctg acatcattat ctttaaccct gatgtagaat
      301 ggaaagtgga taggaattca ttcctttcca ggggcaaaaa ctcgccattc catgggtggg
      361 tattaaaagg gaaaaacctc cttaccctgg ttgatggtaa gattgtctac agggataaat
      421 ctttcaaagc ttaactgcgg gcttttttct ttttctcaag ccttgcttct ttttcctgct
      481 cgtagatatc ttcggtaagg gtagccacgc gctcacgcca ccggggatca ttctggttca
      541 tttcaacctc ttcataaaaa tctaatatac tgtggttcac cagcttgtaa gggttgtcat
      601 cggcttcaat atcaaaagaa acatgccgaa gctcatggcg caggattttt tccctgtctt
      661 cgtcagtaac gtggtcccag catttcttat caagggtgat gataaaatca tagccttctt
      721 cagcattcgc attttttgtg aggtgcttca ccaggtcatt cgccttgaga atccttccca
      781 caacaatccg acctcctgac tttctcttct taagatcaaa aagaaccctg atttttgcat
      841 ttttcagttc ttgaaaatgc tggcttttga cttttctgaa catttccata acagattctg
      901 ttacatcttc atagcgtatt gccatacagt acctccagag ggtcagtctt ttgtatcaac
      961 gcaaggcaaa ctaccatcag ctgaggatga aaatcaagga aagaaaaagg gtgaaggcaa
     1021 taggctatac atgcaatagc agagccggta cacaaccgtt gggtttaagc tgatagtcag
     1081 aacacaacgt atgcatgctg tctataaatt gctgctgatc atatcgtaaa ataggcccgt
     1141 gcaatttcaa cgtctttcct gattcggtcc ttcagctcat ccacactgct gaatttaacc
     1201 tcatcacgaa tcctgtccag gaaatacagg gcaaccttct gattgtagag atcctgggag
     1261 aaatccataa tgtaggtctc aatggtgagg ctttgaccat caaaggtagg attgaatccg
     1321 atatttgtca cagccggcag tctctttccg ccaatctcca ctgagctgac gtaaacgccc
     1381 cgccccggga ttaagggaaa ctcagtctgc agattgattg ttggaaaacc aatcgttctt
     1441 cctctcccgt cacctgccat gaccgttcct cctatcatat agggtctgcc gaggtgcaga
     1501 cctgcttttt taacatcacc ttcctggatc atcctcctga ttctattgct tccaaccttt
     1561 tcattatcca gggttattgc ttcctgaata tcaaagaaaa aaccatattg agtcgatttg
     1621 gcagccaggt actcgacatt cccttttccc cctttgccaa acttaaaatc ataacctaca
     1681 atcaagcctt taatgccaag tttttgtaca agaatatttt ccacaaagct ttcaggactg
     1741 atcagatgga attcatccgt gaaaggaatg atgaacatga catcaatccc cgcctcctct
     1801 attagtcttc tttttaccgg caatggcgtt attaggccga tgaacctgtc aggctttaat
     1861 attgtcaggg gatgggggtg gaatgtcatg agcatggatg ttcccttgtt ctcgcgggct
     1921 tttatctcta ctctttctat aatttttcta tgtcccagat gcaaaccatc atagttgcca
     1981 atggttagaa taggatttgc aaacttctct tcaaaatcga gtgactcaaa aacctgcata
     2041 ggcacctctt gacatcttca gcatatcaat atataacaac aaatatagtg ccgaagtggc
     2101 ggaattggta gacgcgctag attcagggtc tagtaggcaa tatgcctgtg tgggttcaag
     2161 tcccgtcttc ggcattttct tttaacacat tttccgctca tgtgatagcg tttgcaggaa
     2221 aacgttcctg cccttaacat attaaaaatt gttgacaaat tcccttattt aagataaaag
     2281 attgtgaaaa ttttttcaaa tgcaaagaaa ggagagtgct atgagggaag cggtaatagt
     2341 atcatgtgca agaacagcca taggccagtt cggtcagtca ctgaaagaca ttccggtagt
     2401 tcagttgggg ggaatagcaa tcaaggaggc cattaagagg gccggcatca gaccctcaaa
     2461 gaataaggat aaagagtttg ctcccgatat ctttggtgga aagaccgata cagaccttga
     2521 ggcaaaatat tacgactttg atcctggcct gaaagaggtc attattgatg aagtaatcat
     2581 gggcaatgtc cttcagggtg gccttggaca gaactctgca cgccaggcga gcatcaatgg
     2641 tggagttcca aaggaaacag cagcatatac cttaaacaaa gtctgttcat cagggctgag
     2701 ggccatcata gccggcatcc attcggttat ggttggagat aatgatgttg ttgttgcggg
     2761 tggtatggaa aacatgagca tggccccctt tgcagtaccg tcagcaaggt ggggtgccag
     2821 gatgttcgat accaagatga tagacctcat ggttctcgat ggtgtctggg agatttttta
     2881 tggctatcat atgggcgtta ctgccgagaa tatcgcagca cgatacggta tatccagggt
     2941 agaacaggat accttcggtc tcattagcca ccagcgggcc agaaaggcta tcaaagaagg
     3001 actcttcaaa gacgagattg tacctgttat aatcccccag aagaaaggcg atcccatcat
     3061 ctttgatact gatgagagac ccatggacac aaccttagaa aaaatggcaa aactcgcacc
     3121 tgctttcaaa aaagacggca gcgtcactgc gggtaacgca tcaggactta atgactgtgc
     3181 ttcagcggcc gttattatgt cgagggaaaa agcaaatgaa ctgggcatca aacccctcgg
     3241 caaattggta tcatgggcaa acggtggcgt tgatcctgca tatatggggc tcggcccggt
     3301 tccggccatc agaaaggctc tgaagaaagc cggccttacc ctcgaccaga tggatgttat
     3361 tgagctcaac gaggcctttg cttcccagtc gatcgcatgt atcagggagc tcggggtcga
     3421 tacggagaag tgcaacatgt tcggcggcgg agtctccctc ggacacccca ttggatgtac
     3481 aggtgcacgg cttgtgacaa ctgccttcta tgcaatgcag agaatgaata agaaatatgc
     3541 tctcatctcc atgtgcatcg gtggcggtat gggtctggca gcaatcttcg aaagagaggc
     3601 ataagagatg gagtacaaaa atcttttact cgaaatcaag gatggcatag cggttgtcaa
     3661 aattaataga ccgaaggcct taaatgcgct caattcagag acccttgacg agctcaggca
     3721 cgcaggagat gcaatcaatg cgaataagga tgtgtgggtt gtaattatca caggtgaagg
     3781 cgataaagcg ttcattgcag gggcagacat ccttgagatg aaggatatga acccatatca
     3841 gggtatgata ttctctcaga gggggcacga agcctttgca gcttttgaga atatgaacaa
     3901 gccggtcatt gcagcagtaa atggctttgc tcttggcggc gggtttgagg tagctttaat
     3961 gtgcgacatc atctatgcct cagacaaggc taaggtcggg tttcctgaga cgacacttgg
     4021 gatttttccc ggttttggcg gaactcagag aacagcaaag ctttgtggtc ttgccaaggc
     4081 aaaagagctc atttttaccg gaaaaatgat cagcgctaaa gaagcttatg aaattggcct
     4141 tatcaataag gtagtacccc atgaagaact ctggaaggaa gtaatggccc ttgcagagaa
     4201 gatcaggtcc aacggtccta tctccatggg tctggcaaag gcattgatca acaagagcct
     4261 ctttctcgat atggaatccg cgctcatgct ggaagcaaaa gactttggtt tatgttttgg
     4321 cacaaaggat cagaaagaag gtatgacagc ttttgttgaa aaaagaaaag ccacctttac
     4381 aggtgaataa aaacatttaa ggaggagact gtgaagatag tagcatgcgt aaaacaggtt
     4441 cccgatactg aagctgaggt aaaatgggac atcccgggcg gtaaactgaa aagagatgct
     4501 atggattgtg tgacaaatgc atttgacgag tatgccattg aggaagccct tctcacacgg
     4561 gaaaaccatg atggagagat tgttgcaata agtatgggtc cggaaaaagc tatggacgtt
     4621 ttgagaaact gccttgccct taccgtgaat gaggtaaagc tttgcactga tgcagcgttt
     4681 gaaggttccg atacctttgc aaccgcatcg gttcttgcgg cagctatcaa gaaggttggt
     4741 gatgtagaca tcgtttactg tggaaaacag tcaactgacg gaaacactgg tgttgttggg
     4801 gctgccctgg cttcaattct tggatggagc cagcttacct atgtttcaaa aatccgctca
     4861 atcgatggag caggcaaaaa gattgtcgtg gaaagggcaa tagaaggcgg tactgaggtc
     4921 gttgaggcaa agcttcctgc agttgtatcg gtcctcaaag gcattaatga gccc
//
LOCUS       DAOOMC010000162         4963 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdus sp. isolate AMR_MDS_2685
            NODE_20441_length_4963_cov_5.204768, whole genome shotgun sequence.
ACCESSION   DAOOMC010000162 DAOOMC010000000
VERSION     DAOOMC010000162.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864760
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdus sp. (wastewater metagenome)
  ORGANISM  Syntrophorhabdus sp.
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae; Syntrophorhabdus.
REFERENCE   1  (bases 1 to 4963)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4963)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,729
            Genes (coding)                    :: 2,708
            CDSs (with protein)               :: 2,708
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 7 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4963
                     /organism="Syntrophorhabdus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20441_length_4963_cov_5.204768"
                     /isolate="AMR_MDS_2685"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2699756"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(103..1266)
                     /locus_tag="PK125_12530"
     CDS             complement(103..1266)
                     /locus_tag="PK125_12530"
                     /EC_number="2.6.1.83"
                     /inference="COORDINATES: protein motif:HMM:NF006756.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LL-diaminopimelate aminotransferase"
                     /protein_id="HPB38971.1"
                     /translation="MVKPASRIENIPPYLFARIDKKKAEARKKGLDLIDFGVGDPDIP
                     TPQNIITRMKEAVDDSANHRYPSYEGMFEFRKAVADWYKKRFSVDLNPADEVVALIGS
                     KEGIAHMPWAYIDNDDIALIPSPGYPVYKIMTLLTGGNPYIMPLKEENHFLPKYDDIP
                     SSILDKAKIMFLNYPNNPTGAHADDDFYERSLWIAREYDILICHDAAYSEIAYDGYTP
                     RSILEFDKNKKYCVEFHSLSKTYCMTGWRIGFVVGNKEAVSNLGKLKTNIDSGVFQAI
                     QYAGIEALTGDQSSVEIMKKTFQKRRDMVVNGLNSIGIHVEKPKATFYIWAKVPKGYT
                     SADFCEKIIEEQGIVVTPGSGFGEEGEGYFRISITINEKRIAEAIERLKMVRF"
     gene            1463..1918
                     /locus_tag="PK125_12535"
     CDS             1463..1918
                     /locus_tag="PK125_12535"
                     /inference="COORDINATES: protein motif:HMM:NF037131.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB38972.1"
                     /translation="MDVILQAIPYDFSICERPYEEIAKEAGISEDGLINVLKDLKVMG
                     VIRRIAAILYHRRAAYTHNAMVVWEVTQDDVEKIGQTMACFPEVSHCYEREKGDYWGY
                     NLFTMIHAKSLEGCTDIARRIAEKTGIKRYEMFLSKREFKKTSLMVNNE"
     gene            1911..3206
                     /gene="hemL"
                     /locus_tag="PK125_12540"
     CDS             1911..3206
                     /gene="hemL"
                     /locus_tag="PK125_12540"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0042286 - glutamate-1-semialdehyde
                     2,1-aminomutase activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HPB38973.1"
                     /translation="MNRDKSEALFKKAINLIPAGVNSPVRAFRSVNENPFYVKRAKGA
                     YLYDVDENEYLDYVSSWGAIILGHADEGLVNAIKTAVEDGTSYGACHPGEIDIAELII
                     EAFPSIELLRLTSSGTEATMSAIRLARGFTKKQGIIKFRGCYHGHVDSLLVKAGSGLA
                     TFGTPDSGGVPEDLAKHTYVAEFNHIETVKRIIREHKDIACLIMEPIMGNMGVILPEG
                     DFLKDVQEMCQQSGILLICDEVITGFRVTYGGAQHIYNIDPDITCLGKIIGGGFAIGA
                     FGGKRRIMESVAPLGDTYQAGTLSGNPVAVRAGVYTLRYLRDHKELYDLMRQRIELMR
                     DYILKIAKKYDIPYYINATTGMFTGFFSTGSVNDYEAAATCNRKAYERFFKQMLSEGI
                     FFAPSQFEASIVTLCHKEREISKTVDSYEKVFRAMASQL"
     gene            3221..4339
                     /gene="alr"
                     /locus_tag="PK125_12545"
     CDS             3221..4339
                     /gene="alr"
                     /locus_tag="PK125_12545"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HPB38974.1"
                     /translation="MANTNRAVAYIDLSILEENYKTIKSKVSPGTEVLCVVKADAYGH
                     GAVEVCRRLESLGVFYLGVATIDEAVELRIHDISAPILVMSGIMPWDDVSVFLRYNVT
                     PVVYELSGLMKLCSMATAFLTPLKIHLKFDTGMGRLGFMPDNASTIINLLAGVDNIQI
                     EGLMSHFACSELRDEDGIRQIDSFKKVIEYFSNNGIRPKIIHMANSGAIISYPEANFN
                     MVRTGICLYGSHASKELKDQLPTRQVMKFVSKIALIREFPAGYALSYGRTYITKRKTR
                     AAYIPVGYADGYPRALSNKGSVLIHGKRCDIIGRVCMDWFLVDITDNDHMQVNDEVIL
                     LGESTGDCISADEIAEIAETIPYEILCKISRRVTRTYE"
     gene            4340..>4963
                     /locus_tag="PK125_12550"
     CDS             4340..>4963
                     /locus_tag="PK125_12550"
                     /inference="COORDINATES: protein motif:HMM:TIGR00056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPB38975.1"
                     /translation="MVKALFAPLERFVRESGRMSIMLAESIFFAFRPPFKMNYIFKQM
                     EFIGVNSVFVIVLTGAFTGMVLALQSYYGFRKFGSEGLVGATVALSMTRELGPVLTSL
                     MVTGRAGSAMAAELGTMRVTEQIDALTVMALNPIKYLVTPRIIASFLMLPLLTVIADF
                     VGIIGGYLVGVKLLGINEGAYIDKTITFLELDDIYNGLVKAAVFGIIL"
BASE COUNT         1437 a          978 c         1103 g         1445 t
ORIGIN      
        1 taaactactc gcacaaagaa atgcagattt gtccaatttc aaaaaaactt gacattagga
       61 ttattttgga cagcagtgac gcactactga tcacggcttg tttcagaacc ttaccatctt
      121 taggcgctct atcgcttcag ctatacgttt ttcattaata gtaatggata tgcggaaata
      181 accttctccc tcttcgccga aaccgcttcc tggcgtcaca acaattcctt gctcctcaat
      241 aattttttca cagaaatcag cgcttgtgta accttttgga acctttgccc agatataaaa
      301 agttgccttt ggtttttcaa catgaatgcc tatggagttg agcccattaa ccaccatgtc
      361 cctgcgcttc tgaaaagttt ttttcataat ctctacagag ctttggtcgc cggtcagggc
      421 ctcgatccct gcatattgta tggcttgaaa cacaccggaa tctatattgg tttttagttt
      481 accaagattg gaaacagctt ctttgttacc gacaacaaag ccgattctcc agcctgtcat
      541 acaatatgtt tttgaaaggg aatgaaattc aacgcagtat ttcttgtttt tatcgaattc
      601 gagaatactc ctcggagtat atccatcgta cgcaatttca ctatatgcag catcatgaca
      661 gatcaggata tcgtattccc tggcaatcca gagggatcgc tcataaaaat catcatcggc
      721 gtgagcgcct gtagggttgt tcggatagtt caaaaacata atcttagcct tatccagtat
      781 ggatgatggt atatcgtcgt actttggcaa aaaatggttc tcttctttca acggcataat
      841 atatggatta ccgcctgtaa gaagagtcat tattttgtat accggataac ctggggatgg
      901 aataagggca atatcgtcat tgtcgatata tgcccacggc atatgagcaa tgccttcttt
      961 tgatcctatg agggcaacaa cttcatcggc tggatttaaa tcaacagaaa atcgtttttt
     1021 gtaccagtca gcaacagcct ttctgaattc aaacattcct tcgtaactcg gatagcgatg
     1081 attggcagag tcgtcaacag cctctttcat cctcgtgata atgttttgag gagtagggat
     1141 gtcaggatct ccaacaccaa aatctatcag atcaagacct ttttttcttg cttcggcctt
     1201 ctttttatca atacgagcaa atagataagg tggtatattc tcaattcttg aagctggttt
     1261 caccattacg tgcccctcct taatattatg aaatctaccg ggatttcagt ttgacttcgg
     1321 tcaaaaaaac agaaccatat tttatactat aattaatgac atggttgtat ttcttttttc
     1381 aagtccccgt ctcatatgag aaagctggtt tttatttgca aacccccggt gtatgtgtaa
     1441 aaatagaccc atgaaaaagg acatggatgt catattacag gccatcccct atgatttttc
     1501 aatatgtgaa agaccatacg aagagattgc aaaggaggcc ggtatcagtg aggacgggct
     1561 catcaatgtg ttaaaagatc tgaaagtaat gggcgttatc agaagaattg ctgccatact
     1621 ctaccacagg agggcagctt acacccataa tgccatggtc gtatgggagg ttacccagga
     1681 tgatgtagaa aagatcggtc agaccatggc atgtttccca gaggtgtctc actgttatga
     1741 gcgggagaaa ggagactact gggggtataa tcttttcaca atgatacatg caaaaagcct
     1801 cgaaggctgc actgatattg cccggcgtat tgcagaaaaa acaggtatca aaagatatga
     1861 aatgtttctc agcaaaagag aatttaaaaa gacttcactc atggtgaata atgaatagag
     1921 ataaatctga agccttattc aaaaaggcaa tcaatcttat acctgccgga gtcaatagtc
     1981 ctgtcagagc attcagatca gttaatgaaa acccttttta tgtgaaaaga gcaaaagggg
     2041 catatcttta cgatgttgat gaaaacgaat accttgacta tgtgtcttcc tggggagcaa
     2101 taattcttgg tcatgcagat gagggcctgg ttaatgctat aaaaacagca gttgaagatg
     2161 gtacaagcta tggagcgtgt catcctggcg agattgatat agcagaactt atcatcgaag
     2221 cttttccctc catagaactc ttgaggctta caagctcagg tactgaggct acaatgagtg
     2281 ccatccgcct tgcacgaggt tttacaaaaa agcagggtat cattaaattc agggggtgct
     2341 accacggcca cgtggacagc cttctcgtaa aggcaggttc agggcttgcc acatttggaa
     2401 cacctgatag tggcggtgtg ccggaggatc tggcaaaaca tacctatgtg gcagaattca
     2461 accatattga aactgtaaaa agaattattc gggagcataa ggacattgca tgtcttatca
     2521 tggaacctat catgggcaat atgggcgtca tcctccctga aggagatttt ttgaaagatg
     2581 ttcaggaaat gtgtcaacaa tccggcatcc ttttaatctg tgatgaagtg ataaccggat
     2641 tccgggtaac ctacggtggt gcccagcaca tttacaacat cgatcctgat ataacgtgtc
     2701 tgggaaaaat aattggcgga ggttttgcca ttggggcctt tggcggcaaa aggaggatta
     2761 tggagtctgt cgcaccgctc ggtgatacat atcaggccgg aacgctctca ggcaaccctg
     2821 ttgcagtaag ggcaggggtc tacacccttc gatacctccg tgatcacaag gagttatatg
     2881 atctgatgag acagagaatc gaattgatga gagattacat tctcaaaata gcgaagaaat
     2941 atgatattcc gtactatatt aacgctacta cagggatgtt tacaggcttt ttttctactg
     3001 gcagtgtcaa cgattatgaa gctgcagcaa catgtaacag aaaagcctat gagagattct
     3061 tcaaacaaat gctgtcagaa ggcatatttt ttgcaccaag tcaatttgaa gcgtccattg
     3121 tgacgctgtg ccataaggag agggagattt ctaaaacagt tgattcttat gaaaaggttt
     3181 ttagggcaat ggcatctcaa ctatgaggta ggtacataca gtggcaaaca ctaacagagc
     3241 cgttgcctat attgatcttt caatccttga agagaattat aagacaataa aatcgaaagt
     3301 ctcacctggg actgaagtac tgtgcgttgt aaaagcagac gcatatggtc atggtgcagt
     3361 tgaagtatgc cgacgccttg aatcccttgg tgtattctat cttggtgttg ccactattga
     3421 tgaagcggta gaattgcgaa ttcatgatat tagtgctcct atacttgtca tgagtggcat
     3481 catgccatgg gatgatgttt ctgtatttct tcgttacaat gttactcctg ttgtatatga
     3541 actttccgga ttaatgaaac tttgttccat ggcaacagct tttttaaccc ctttaaagat
     3601 tcatctcaaa tttgatacag gcatgggtag acttggattt atgcctgata acgcctccac
     3661 aataataaat ctcttagctg gtgtggacaa cattcagatc gaagggttga tgagccactt
     3721 cgcctgttct gagcttagag atgaagatgg cattaggcag attgattctt ttaaaaaagt
     3781 tattgaatac ttttcaaata atggtattcg acccaagatt atccacatgg caaacagtgg
     3841 tgcaatcatt agctatcctg aagctaactt caacatggtg cgcactggca tctgcctcta
     3901 tggatcacat gcctcgaagg agctaaaaga tcagttgcct actagacagg tcatgaaatt
     3961 cgtttcaaag attgcgctca ttcgtgaatt cccggcaggg tatgcgttaa gttacggtag
     4021 aacatacatt actaaacgta aaacgagagc tgcatatatt cccgttggtt atgcagatgg
     4081 ataccccagg gcgctttcca acaaggggtc tgtcctgata cacggtaagc gttgtgacat
     4141 tatcgggagg gtttgtatgg actggtttct ggttgacatc actgataatg accatatgca
     4201 ggtgaatgat gaggtaattc ttcttggaga gagcacaggt gattgtatta gtgctgacga
     4261 aattgctgaa atcgcagaaa caattcccta tgaaatcctg tgcaaaatat cgagaagggt
     4321 aaccaggaca tatgaataaa tggtaaaagc tttgtttgca ccactggaga gatttgttcg
     4381 ggaaagcgga agaatgagca ttatgctcgc agaaagcatt ttttttgctt tccgaccacc
     4441 cttcaagatg aactatatat ttaaacaaat ggaatttatt ggtgttaact cggtctttgt
     4501 aatagtgctc accggggctt tcacaggtat ggtgcttgct cttcaatcat attacgggtt
     4561 cagaaaattt ggctcagagg ggcttgtcgg tgctaccgtc gctttaagca tgacgcgtga
     4621 attggggcct gtccttacaa gtcttatggt aacaggaagg gctggttctg caatggcagc
     4681 tgaattggga actatgagag ttacggaaca gatagatgcc cttacggtaa tggccctcaa
     4741 cccaatcaaa tatctggtca caccgagaat aattgcatcc ttccttatgc ttccccttct
     4801 tacagtcatc gctgattttg tcggcattat aggtggttac ttggttggtg taaagctttt
     4861 gggtataaat gaaggggctt atattgacaa gacgataaca ttccttgaat tagatgatat
     4921 ttacaacggg cttgtcaaag cagcggtttt tgggataata ctc
//
LOCUS       DAOOMC010000163         4882 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdus sp. isolate AMR_MDS_2685
            NODE_20863_length_4882_cov_6.889372, whole genome shotgun sequence.
ACCESSION   DAOOMC010000163 DAOOMC010000000
VERSION     DAOOMC010000163.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864760
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdus sp. (wastewater metagenome)
  ORGANISM  Syntrophorhabdus sp.
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae; Syntrophorhabdus.
REFERENCE   1  (bases 1 to 4882)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4882)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,729
            Genes (coding)                    :: 2,708
            CDSs (with protein)               :: 2,708
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 7 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4882
                     /organism="Syntrophorhabdus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20863_length_4882_cov_6.889372"
                     /isolate="AMR_MDS_2685"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2699756"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..210
                     /locus_tag="PK125_12555"
     CDS             <1..210
                     /locus_tag="PK125_12555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB38976.1"
                     /translation="TIIYLTIIALVATGLLAWPGLLSLLTVPIFLRLIRIVRSTEDWV
                     LLDQHGKYVRMLYFFNGLSIIFGVL"
     gene            570..2624
                     /locus_tag="PK125_12560"
     CDS             570..2624
                     /locus_tag="PK125_12560"
                     /inference="COORDINATES: protein motif:HMM:NF012602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HPB38977.1"
                     /translation="MEVIKTDCGLCINCCGVNAYVENGKLVKVEGTKENPISKGRLCP
                     KGDRLMDMLYSPSRLKYPMKKVNGHFQRISWDDAIGEIATKLQQLKDKYGAHTLATWT
                     GSVGVEHFEMAAFNQRFKGAFGTPNFFNPEGMCFRTRILARQITFGRYPVEEPVNANC
                     ILLWGRNPDESYPPVGRIVRERVEAGAKLVTIDPRRIPISRLGIYLQPRPGTDGAIAL
                     AMMNVIIEEHLWDKEFVEKYTFGFDKLAEHVREFTPEKAEMISSVPASEIVRTARIFA
                     TTKSACILEGVGHMNQFANGLQTNRAYSILMAITGNVDVPGGWVTCPQVRLADLRLPQ
                     EENNLGWDEFPVFHQFSKRPPPYGSAGLMTEVMKTEKPYPIKAFISSGSNPLLTFPDT
                     KLFLEGIKNLELFVNIDPNMTETGYLADYVLPACTFLEETGIGGFPYGISYCEPYVML
                     RKKVVEPLYESKPIWLIWTELGRKMGYAEHFPWNTDEEVAEHFFSTSGVTMKDLTDHP
                     AGVYFGKKEYRLFEKVPFATTSGKIELFSSRMVELGHSGLPVHMEPHQSAVANPEIVK
                     EYPEILLTGNRQVEYINAQMHDVTALRARIPDAEVEVNPITAAKYGISHGELVGIETP
                     RGSIRMKANVTQDIKPGVVSVPHGWAEANCNILLDAQLKDPVSGYITMNSNACRLVKL
                     RI"
     gene            2779..3396
                     /locus_tag="PK125_12565"
     CDS             2779..3396
                     /locus_tag="PK125_12565"
                     /inference="COORDINATES: protein motif:HMM:NF014693.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formylmethanofuran dehydrogenase subunit E
                     family protein"
                     /protein_id="HPB38978.1"
                     /translation="MSNILSYNFNEFIKMAASFHGSPAPGVLIGGFMVDYAYKHLPKG
                     GEYDVICETSKCLPDAVQLLTPCSIGNQWLKIVDVGRYALTFYEKHSGRGIRVYLDWK
                     LLEKFPQIKGWYLKLIPKKEQDADSLLREIMEAGSNICSLEEITVIIDPVNKRKNSSI
                     TICPSCEEAYRSEDGAICPGCQGGTLPYSREKNLRHTSVDFNLSA"
     gene            complement(3474..3878)
                     /locus_tag="PK125_12570"
     CDS             complement(3474..3878)
                     /locus_tag="PK125_12570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB38979.1"
                     /translation="MRVPGTIEKHDPEHQAHVCRFLGIPLHEYEDWLACYNIIHWRRF
                     NNFLMELRDKIEECKRNKDQPCLTLNETLRLYGIEDRILNPIQEEHLLELTNRILDEH
                     DLNWFLENHERIIEELEETFEKQNYEQHQGSA"
     gene            complement(3923..4723)
                     /locus_tag="PK125_12575"
     CDS             complement(3923..4723)
                     /locus_tag="PK125_12575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB38980.1"
                     /translation="MKSGVTRIMRRIDSKTVFVASIMFALVLMVNVSTGGEPTKDGLV
                     EYIGAQKNMFETGKADGVLNLKFFQGKENMYAVGPAALLNGEITILNSKPYVAKVREN
                     DYHVDNTLNHDALFIVWAQVPRWKDIPVPESVNTYVQLQNFVKDQAAALGMDTSKPIP
                     FQFSGTPVEIVWHINCDKTDNKPITRELFAKSKAIYTMKNEPVDIIGFYSERHYGIFI
                     SQYAPAIKPGSGDKNAVHMHLISRTTKATGHIDDITLGKGMTLRLPDR"
BASE COUNT         1343 a         1095 c         1064 g         1380 t
ORIGIN      
        1 actattattt accttactat catcgccctc gttgcaaccg gacttctggc atggcctggt
       61 ctcttatcac tcctgacagt ccctatcttt ttgaggttaa tccgtatcgt gaggagcact
      121 gaggattggg ttctccttga ccagcacgga aaatacgtta gaatgctcta ctttttcaat
      181 gggttatcaa ttattttcgg cgtactgtaa cacagaacac gcaacaatat tcataaatag
      241 aacctggttt ctctctccct gccattcatg gatgatacag ggttgcattc aataaccagt
      301 acgtgttgtt atgaccatac tgattacacc acagcattcg tatgatgatg tttcataatt
      361 gcattaatag ttttgttatt gcctttcctg ttatttttgg ttataatttc cctaacttta
      421 tgatggcata aacttttacg aaatatatgg ccacaaacaa tgggttattg ccagtcagaa
      481 ttcagaagga tattcgggta gacgtttgta aaacatagtg cacaagaacc aacaagtcag
      541 aaggtttaaa aatcgttgga ggtagaatta tggaagtgat caagacagat tgtggtttat
      601 gcatcaactg ttgcggtgta aacgcatacg ttgagaacgg caaactggtg aaggtggaag
      661 gcacgaaaga gaatcccatt agtaagggga gattatgccc gaaaggcgat cggcttatgg
      721 atatgctcta ttcacccagc cgtttaaaat acccaatgaa aaaggttaac ggccatttcc
      781 agcgaatatc ctgggatgat gctataggag agatagctac caaactgcaa caactgaaag
      841 acaagtatgg cgcacatacg cttgcaacct ggactggttc agtcggcgtt gaacatttcg
      901 agatggcagc atttaatcag cgtttcaaag gggccttcgg cacacctaac ttctttaatc
      961 ccgaaggtat gtgctttcgc acccgcatcc tggctcggca aataaccttc ggacgctatc
     1021 cagtagaaga acctgtgaat gctaactgta ttctactctg gggtcgcaac ccggatgaaa
     1081 gctacccccc tgtaggcagg attgttcgtg aacgggttga agccggtgca aaactcgtaa
     1141 caattgaccc ccgtcgcatt cccatttcca gattgggaat ctatttgcag ccacgtcccg
     1201 gtactgatgg cgccatagcc cttgccatga tgaatgtcat catagaggaa cacctatggg
     1261 ataaggagtt tgttgaaaaa tataccttcg gttttgacaa gctggcagag catgtacggg
     1321 agtttacccc tgaaaaggct gagatgatca gcagtgttcc tgcttcagag attgtacgta
     1381 cagcacggat ctttgccacg acaaaaagcg cttgtatcct tgaaggggtg ggccatatga
     1441 atcagttcgc caatggcctc cagacgaaca gggcttactc aatactgatg gcaataaccg
     1501 gcaatgttga tgtgcctggt gggtgggtaa cctgtccgca ggtcaggctg gctgatctgc
     1561 gtctgcccca ggaggaaaac aaccttggat gggacgaatt tcccgtattc catcagtttt
     1621 ccaaacgtcc acccccctat ggttcagcag gattgatgac cgaggtcatg aaaacagaga
     1681 agccttaccc cataaaagct tttatcagct caggcagcaa cccgcttctg accttccctg
     1741 ataccaaatt gttcctggaa ggaataaaaa accttgaact ctttgtcaac attgacccca
     1801 atatgactga aacagggtac ctcgctgact atgtcttgcc tgcctgtacc tttctggaag
     1861 aaaccggtat tggcggattc ccctatggca tatcctactg tgagccttat gtgatgctgc
     1921 gcaaaaaagt tgtagaaccg ctctatgaga gcaaacccat ctggttgatc tggactgaat
     1981 tgggccgtaa aatgggttac gctgaacatt ttccctggaa tacagatgaa gaagtggcgg
     2041 aacacttttt ctcaaccagc ggcgtcacca tgaaggacct cactgatcat cccgcaggtg
     2101 tatactttgg taaaaaggaa tacaggctct ttgaaaaggt accttttgca accacgagcg
     2161 gcaaaataga actgttttcg tcgcgaatgg tggagttggg ccattccggg ctgcctgttc
     2221 acatggaacc acaccagagc gcagtagcga atcctgaaat agtaaaggaa taccccgaga
     2281 tcttactcac cggcaacaga caggttgagt atatcaacgc ccagatgcat gatgtgacag
     2341 ctttacgagc aagaatacct gatgcagaag tggaagtgaa tcctatcaca gctgcaaagt
     2401 atggcatcag tcacggtgag ttagtcggta ttgaaacccc aaggggttcc ataaggatga
     2461 aagcaaatgt cacccaggac attaaaccag gggttgtatc tgtgcctcat ggatgggctg
     2521 aagcaaactg taacatactc ttggatgcgc agctaaaaga tcccgtttca ggttatataa
     2581 caatgaacag caatgcctgt cgccttgtca agctgcgcat ttgagtgacg ctttcttgct
     2641 atttgaaact ttgtgcccgg ttattccact ccgggcacaa gactttaaat tcagtatgat
     2701 atcttcgacc gaagatgata aaaaaaggca aagcattttt ggatgtaagc cttttgcttc
     2761 tcaacatagg agaaaatagt gtcgaatatc ctttcatata atttcaatga gttcataaaa
     2821 atggctgctt catttcacgg aagtcctgcc cccggtgttt taataggtgg ttttatggtg
     2881 gattatgcgt ataagcatct tcccaaaggg ggcgagtacg atgtcatatg cgagacgtca
     2941 aagtgtttac ccgatgcagt tcagcttctc acaccgtgct ctataggaaa tcaatggctc
     3001 aagattgtgg atgtcggccg ctatgcccta accttttacg aaaaacactc gggaaggggg
     3061 atccgggtct accttgattg gaagctcctt gaaaagtttc cccagatcaa aggctggtat
     3121 ctaaagctta ttccgaagaa ggaacaagat gctgattcac tacttcgaga aataatggaa
     3181 gcaggatcaa atatctgcag ccttgaagaa atcactgtta ttattgatcc cgtaaacaag
     3241 cggaaaaact cttccattac catctgtccg tcctgtgaag aggcgtaccg cagtgaggat
     3301 ggggccattt gtcctggctg ccagggagga acattgccct acagccgaga gaaaaacctt
     3361 cggcacacca gcgttgattt taacctctcc gcataagcaa cattttattc agtggaaatt
     3421 cgaaacttga tagtcctaaa aagaagcagt gatcagagtt gagtagatta atactatgct
     3481 gaaccttgat gttgctcgta gttttgcttc tcgaaggttt cttccaactc ttctattatt
     3541 ctttcgtgat tttccaagaa ccagtttaag tcatgttcgt ccaatatccg atttgtcaat
     3601 tctaaaaggt gctcttcctg tattggattt aagatccgat cttctatccc atataatctc
     3661 agagtttcat ttagtgttag acatggctga tctttgtttc ttttgcactc ctctatcttg
     3721 tccctcagct ccattaagaa gttgttaaat cttctccagt ggatgatatt atagcacgct
     3781 aaccagtctt catattcatg aaggggaatg ccgagaaagc gacagacatg tgcctgatgc
     3841 tcaggatcgt gtttttctat agtgcctgga actctcactc cagaaaccca ttgtggtgag
     3901 tacagtttcc tagaaacatt atttatctat cgggcaggcg aagtgtcatg ccttttccaa
     3961 gagtaatatc atcaatatgt cccgtagcct tcgttgtgcg tgaaataagg tgcatatgca
     4021 ctgcgttctt atcccctgaa ccaggtttta tagcaggagc atactggctg ataaatattc
     4081 cgtaatgtct ttcggagtaa aaacctatta tatcgaccgg ttcatttttc atagtataga
     4141 tggccttcga tttggcaaac aactctcttg taatcggttt gttgtctgtt ttgtcgcaat
     4201 tgatgtgcca tactatctct accggtgttc cactgaactg aaacgggatt ggtttggagg
     4261 tatccattcc caacgcagca gcttgatcct taacaaaatt ttgaagttga acataggtgt
     4321 tgacagattc tggaacaggg atatccttcc agcggggaac ttgcgcccat acaataaaaa
     4381 gtgcatcgtg gttcagcgtg ttatcgacat ggtagtcgtt ttcacggact ttggcgacat
     4441 acggctttga attcaatatg gtaatttctc cattcagcaa ggctgcagga cccacagcat
     4501 acatgttctc tttgccctga aagaacttca ggtttagaac cccatcagcc ttccctgttt
     4561 caaacatatt cttctgggcc ccaatatatt caaccagacc atcctttgtc ggctctccac
     4621 ccgtacttac attaaccata agaacgagag cgaacattat cgacgcaaca aaaaccgtct
     4681 tactgtcaat tctcctcatt atacgagtga caccgctttt cattcgttgt tcctccttaa
     4741 aaaatttaca atctcatgtg cttccttaca cagcctttgt aatcgacacc gcctcggtat
     4801 ataaaaaatc ttactctctt tgaattctgt agccacatta tatcttttga agattgtcca
     4861 gtcaagtcac aataagtttt at
//
LOCUS       DAOOMC010000164         4865 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdus sp. isolate AMR_MDS_2685
            NODE_20958_length_4865_cov_6.136798, whole genome shotgun sequence.
ACCESSION   DAOOMC010000164 DAOOMC010000000
VERSION     DAOOMC010000164.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864760
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdus sp. (wastewater metagenome)
  ORGANISM  Syntrophorhabdus sp.
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae; Syntrophorhabdus.
REFERENCE   1  (bases 1 to 4865)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4865)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,729
            Genes (coding)                    :: 2,708
            CDSs (with protein)               :: 2,708
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 7 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4865
                     /organism="Syntrophorhabdus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20958_length_4865_cov_6.136798"
                     /isolate="AMR_MDS_2685"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2699756"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            256..1623
                     /locus_tag="PK125_12580"
     CDS             256..1623
                     /locus_tag="PK125_12580"
                     /inference="COORDINATES: protein motif:HMM:NF024299.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPB38981.1"
                     /translation="MRTQKEWFDLGVDYVRSGKFQDAIDAYKEAIQLEPNNGAIYNNL
                     GAVYGKSGLHEEAIEAYKKAIQIEPNDATAHFNLGLAYRESGMFEEAIESLRRATQIQ
                     PNKAMAHYGLGLAYRESGMYQQAIEAFRRAIRIEPNNAAVHFNFGLAYRESGMFREAI
                     EAFKEAIRIQPNKAMAHCGLGLAYRESGMYQQAIEPFRRAIRLEPDNATAHFNLGLAY
                     RESGMFEEAIESLRRATQIQPNNAVAYNNLGNTYVESGMYEEAISAYKLSIKIQPDSS
                     ATYNNLGNAYGRLHLRNDEMEAYKQAILIEPNDATAHFNLGLAYNASGMYEEAIEAFK
                     EAIRIQPNKAMAHCGLGLAYRESGMYQQAIEAFKGAIQMRPYDARIHYWLGITYGKSD
                     NHKDAIDAYKQAIRIKPDYTDAHYHLGVAYTRLNDRNAALREHSILTKLDEQIALRLF
                     KFIYE"
     gene            complement(1662..2459)
                     /locus_tag="PK125_12585"
     CDS             complement(1662..2459)
                     /locus_tag="PK125_12585"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HPB38982.1"
                     /translation="MIYGATNNPLRSLIEEIRALAALGFDYLELCLDPPYCSPEVLRT
                     QMVEIRSALNNEGLKLPVVHLPTFVWLADIYSSIREASLDEIFKALDVAVEFGAKKAV
                     LHPGYLTGLMSFALDMGRYYAASSLSMILERSADLGIMLCLENMFPRAGHMYRPEEFA
                     EVLSKNPGLMMTLDLGHANIRAPKDQALNFVKVAQGRIGHVHIGDNNGQEDEHLPVGA
                     GKANLVAGLEAVKASGYDGTITLEIFSADRDYLSISLKKVKEIWKNI"
     gene            complement(2654..3874)
                     /locus_tag="PK125_12590"
     CDS             complement(2654..3874)
                     /locus_tag="PK125_12590"
                     /inference="COORDINATES: protein motif:HMM:NF017569.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HPB38983.1"
                     /translation="MFTDFFYTLRQKGVPVTVTEWVSFMEALYNGYFQSSLNHLYYIG
                     RAFLVKSEAYYDMFDIAFQEYFGGISSSPVELDKVMEWLENPLHKLPKLSPEEMAEFQ
                     KKLEEFKKEHDMEELMRQFRERLKEQKERHDGGGKWIGTGGTSPFGAYGYHPGGIRVG
                     GESWMQSAAKVAEERRFRNYRNDIILDVRQTKMALKKLRELKREGSQEELDIPETIDK
                     TAKEGGEIELVFNRSRENSIRLILLMDTGGSMLPYTQLCERLFSAATQANHFKELKYY
                     FFHNCIYQDVYEDIANYKRVPTEKLFSQFHKGYKVVLVGDARMAYSELFDINGCIDYF
                     SYNDTPGLEWLLRIKNHFPHSVWLNPTHKNFWGHYTVDTIGKVFPMFELTVDGLKDAI
                     KSLTGRARPVQFQN"
     gene            complement(3878..4729)
                     /locus_tag="PK125_12595"
     CDS             complement(3878..4729)
                     /locus_tag="PK125_12595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HPB38984.1"
                     /translation="MTQSGQGYRGSDDYIASKPLMEIVNVSIALGKPLVIKGEPGTGK
                     TLLAHSIAEALNKKLLIWNIKSTTKAKDGLYVYDTVQRLNDARFKDKDISDIRQYIKL
                     GKLGQAFKADEKLVLLIDEIDKADVEFPNDLLNELDEMSFHIPELDEEVVATQRPIVI
                     ITSNSEKELPDAFLRRCIFHYIEFPDEEMMERIVKVHYPDIEMKLVREAIKRFYWIRE
                     VDGLRKKPSTSELLDWIKALALGGINVDKITSEIPFLGTLLKNELDTSRFVRLSNAQG
                     GGIFRRR"
BASE COUNT         1356 a         1107 c         1069 g         1333 t
ORIGIN      
        1 aacagagcat cactcaagca tattgttacc atgaacatgg atagataggg tgcaggggga
       61 attataataa tgttggagtg gtgatcataa atatatttct atgcaacaat gattatcaat
      121 tgaggtttta tgttttgtca gctttgaaat attgacaatt ctgcgcaaca gtgtaaaatg
      181 tagatataat aagcataatt taatgtaacg catcagcggg gatttactgt agattaaaca
      241 ggtaaggtgg gtgttatgag aacacaaaag gaatggtttg accttggtgt tgactatgta
      301 cgttcgggca agtttcaaga tgctattgac gcgtataaag aagcaataca acttgagcct
      361 aacaacggtg ctatctataa caacctcggc gctgtttacg gaaaatcagg tctccacgaa
      421 gaagccatag aggcctataa gaaggctatc cagattgaac ctaacgacgc gacagcccat
      481 tttaaccttg gccttgccta ccgggaatca ggtatgtttg aagaggctat tgaatcactc
      541 agacgggcca cacagataca accaaataag gccatggccc attatggcct tggccttgcc
      601 taccgggaat caggtatgta ccagcaggca atagaggcat ttaggagggc cattcggata
      661 gaaccgaata atgcagcagt ccattttaac tttggtcttg cttaccgcga atcaggtatg
      721 tttagggagg ccatagaggc cttcaaagaa gccataagga tacaaccgaa taaggccatg
      781 gcccattgtg gccttggcct tgcctaccgg gaatcaggta tgtaccagca ggcaattgag
      841 cctttcaggc gcgctattcg acttgagcct gataatgcaa cagcccattt taaccttggc
      901 cttgcctacc gggaatcagg tatgtttgaa gaggctattg aatcactcag acgggccaca
      961 cagatacaac caaataacgc agttgcctac aataaccttg gcaatactta cgtggaatca
     1021 ggcatgtatg aagaagccat aagcgcatat aagctgtcca taaaaattca acccgacagc
     1081 tcagccactt acaataacct tggcaatgca tatgggaggt tgcaccttcg caatgatgag
     1141 atggaggcat acaagcaggc tatcctgatt gaacctaacg acgcaacagc ccatttcaac
     1201 cttggccttg cctacaacgc atcgggcatg tacgaagagg ccatagaggc cttcaaagaa
     1261 gccataagga tacaaccgaa taaggccatg gcccattgtg gccttggcct tgcctaccgg
     1321 gaatcaggta tgtaccagca ggcaattgag gctttcaaag gggccatcca aatgcgacct
     1381 tatgatgcaa gaattcatta ctggcttggc attacctatg gaaaatcaga caaccacaaa
     1441 gatgcaatcg atgcatataa acaggccata cggataaaac ccgattatac tgacgctcac
     1501 tatcatctgg gagtagccta cacacgtctg aacgatagaa atgctgcttt gcgtgaacac
     1561 agtattttaa cgaaactcga tgagcagata gctctcagat tattcaagtt tatttacgaa
     1621 taatagaaac cagatcttga tgcagggcct gattgccttg cctatatgtt cttccagatt
     1681 tcctttactt ttttcaggga aatggataga tagtctctgt ccgctgagaa gatctccagt
     1741 gttatagttc catcgtaacc ggatgccttg acagcttcta gccctgcgac aaggtttgcc
     1801 ttaccagcgc ctaccggtag gtgttcatct tcctgaccat tgttgtctcc aatatggaca
     1861 tgtccaatcc gtccctgggc taccttgaca aagtttagag cttggtcttt tggggctcgg
     1921 atattggcat gccccaaatc cagcgtcatc atcagccctg gatttttgct taatacttca
     1981 gcgaactcct ctgggcgata catgtgacct gctcgcggaa acatattttc gagacaaagc
     2041 attataccaa ggtcagcaga cctttccagg atcatactga gagaagatgc agcataatac
     2101 ctacccatat ccagagcaaa gctcataaga ccagtcagat atcccggatg aagtactgct
     2161 tttttcgccc caaattcgac cgccacatcg agggctttga aaatctcgtc cagggaagcc
     2221 tcacgaattg atgaatatat gtcggccagc cagacgaaag tcggtaaatg aacaacagga
     2281 agtttgaggc cttcattgtt cagtgcagag cgaatctcga ccatttgagt ccgaagtacc
     2341 tcaggtgagc aatatggtgg gtcgagacag agctccagat agtcaaaacc aagggctgcc
     2401 agggccctaa tctcttctat cagtgatcga agcggattat tggtagcacc atatatcatc
     2461 aaattaacta caatattctc ctattacatt ttgtgaatta caatgctgaa aaaactgtct
     2521 gggttgaact gggaggatga gcatgccatt gagcattcca gcgtgcaagc gaagtcactg
     2581 aagcagagtg agtatacctg caagagaaag cacgatgcag acctgatttc aatttcattg
     2641 gaagcactat tttctaattt tgaaattgca ccggacgtgc ccgtcccgtg agggacttaa
     2701 ttgcatcttt gaggccgtct acggtgagct caaacatagg aaagaccttg ccgatggtgt
     2761 ccactgtata gtgtccccag aaatttttgt gagtcgggtt gagccataca gagtggggaa
     2821 agtgattctt aatcctgaga agccactcga gtcctggcgt atcattgtat gaaaaatagt
     2881 cgatacaacc attgatatca aaaagctccg aatatgccat ccgcgcatca cccacaagta
     2941 caaccttata tcccttgtgg aactgggaaa agagtttctc cgtaggcact cttttataat
     3001 tagcaatatc ttcgtaaaca tcctggtaga tgcagttgtg gaagaaatag tattttaatt
     3061 ctttaaaatg gttagcctgg gttgcggctg aaaaaagtct ttcgcagagc tgggtgtagg
     3121 gaagcattga cccgcctgta tccataagca aaatgagacg aatagagttc tcccgtgacc
     3181 tgttaaacac gagctctatc tctccaccct cttttgcagt cttgtcaatt gtttcgggaa
     3241 tgtccaattc ttcctgggac ccttcccttt taagttctct gagcttcttc agggccatct
     3301 tggtttgccg cacatccaga atgatatcgt tccgataatt tctaaacctg cgttcttctg
     3361 caacctttgc agcagattgc atccacgatt caccgccaac ccttatacca cccggatggt
     3421 atccataggc accaaaaggc gaggtacctc cagtgccaat ccacttgccg cccccatcat
     3481 ggcgctcctt ctgttctttt aggcgctctc tgaactggcg catgagttct tccatgtcat
     3541 gttctttttt gaactcttcc agctttttct ggaattcagc catttcttcc ggagaaagtt
     3601 ttggtaactt gtgaaggggg ttctcaagcc attccataac cttatcaagt tcaacaggac
     3661 ttgacgagat gccgccaaag tactcttgga acgcaatatc gaacatgtcg tagtaggctt
     3721 cgctcttcac aagaaacgca cggcctatat agtaaaggtg gttgaggctt gactggaaat
     3781 acccgttgta aagagcttcc ataaaggaaa cccattcagt aacggtaaca ggtactcctt
     3841 tttgcctcaa cgtgtagaaa aagtcagtaa acatctgcta tcttctcctg aaaatcccac
     3901 cgccctgagc attggaaaga cgcacaaacc tgctggtatc cagttcgttt ttcagaagcg
     3961 ttcccaggaa tgggatttcc gaagttattt tgtctacatt gataccacct aatgctaacg
     4021 cttttatcca gtcgagaagt tcactggtag agggtttttt tctcagcccg tcaacctcgc
     4081 gaatccagta gaatcttttg attgcttctc tcacaagttt catttcgatg tctggataat
     4141 ggacctttac aatccgttcc atcatttcct catcagggaa ctcaatgtaa tggaatatgc
     4201 aacgccgcag gaaggcatca ggaagttcct tttcagagtt actggtgatg attactatag
     4261 gtctttgggt tgcaacaact tcttcatcaa gttctgggat atgaaatgac atttcgtcca
     4321 attcattgag aagatcgttc gggaactcaa catctgcctt gtctatctca tcgataagaa
     4381 gcaccaattt ttcatcagct ttaaaggctt gccctagttt tcccaacttg atgtactgac
     4441 ggatgtcaga tatatctttg tctttgaaac gtgcatcgtt taacctctga accgtatcat
     4501 atacatagag gccgtccttt gcctttgtgg ttgatttgat attccagatg aggagcttct
     4561 tgttgagagc ctctgcaata ctgtgtgcca aaagtgtttt accagtgccc ggttcaccct
     4621 ttataacaag tggtttcccc agggcgattg aaacgttcac tatctccatt aggggcttgg
     4681 aggcaatgta gtcatcacta cccctgtagc cttgtccgga ctgtgtcatg tttttaagcc
     4741 tccttgtaca aagtgaaaaa tatcacgaat attataaggt tttaggggat gaaagtcaat
     4801 catacagtct gcatgcgatt gtttcgatta gtagtaatac tgtgcaaact aaggtggcat
     4861 ggaaa
//
LOCUS       DAOOMC010000165         4822 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdus sp. isolate AMR_MDS_2685
            NODE_21185_length_4822_cov_7.312775, whole genome shotgun sequence.
ACCESSION   DAOOMC010000165 DAOOMC010000000
VERSION     DAOOMC010000165.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864760
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdus sp. (wastewater metagenome)
  ORGANISM  Syntrophorhabdus sp.
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae; Syntrophorhabdus.
REFERENCE   1  (bases 1 to 4822)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4822)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,729
            Genes (coding)                    :: 2,708
            CDSs (with protein)               :: 2,708
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 7 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4822
                     /organism="Syntrophorhabdus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21185_length_4822_cov_7.312775"
                     /isolate="AMR_MDS_2685"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2699756"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(155..1183)
                     /locus_tag="PK125_12600"
     CDS             complement(155..1183)
                     /locus_tag="PK125_12600"
                     /inference="COORDINATES: protein motif:HMM:NF033581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
                     /protein_id="HPB38985.1"
                     /translation="MKDTFTTPTFADYFVEIRKQSRKNFLDDVNRLIDWKPIEKILKK
                     KYRKVASADGRPAYPPVPMFKLLLLQRWYGLSDPGLEDALSDRISFIRFSGFSLGSTL
                     PDHSTICRFRNTCMELDIYEKLFGEINRQIESHGLLVKEGAIIDATIVESSRRPRKVI
                     EVMAEDRSEEETDTPPPQITYSDDHDATWVRKGKKPYYGYKTHVVVDTKEGFILNGHV
                     TPAHVADTTEFENLMENLFLPDTSPVCADKGYASQKNRDLLAARGYEDAIMHKAARGK
                     PLTFFQRFINRIISAFRYRVEQGIGTLKKHYGFARMRYLGLKKGNMEFLLIAMAFNLK
                     KAARMIET"
     gene            1385..2743
                     /locus_tag="PK125_12605"
     CDS             1385..2743
                     /locus_tag="PK125_12605"
                     /inference="COORDINATES: protein motif:HMM:TIGR02669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIID/LytB domain-containing protein"
                     /protein_id="HPB38986.1"
                     /translation="MAPFSCNPLTHTFTEPSITVGIMDHRKEVAGCFEGYYGVEGVGQ
                     FSGNFTARDKNGAIVLSDANGTEIIHGLKIRCTAQPDSLFHISEVTIGIDFHWERKEE
                     QVFQGNLILALRKDGMIAVINEINLEDYLKSVISSEMNSRAPREFLKAHAILSRSWLM
                     AALNRKKVPHQPREGATKITEHEIVRWYDREDHDLYDVCADDHCQRYQGITKIISKEA
                     IDAVRQTHGQVITSGETICDARYSKACGGLTENYGTAWDDREVHYLTSISDSSVPHRA
                     IRSEEEAEHWISSRPVVYCNTNDTDFLENVLRDFDRETRDFFRWKIEYAREELEMILQ
                     KKSGFDFGTLQNIVPLHRGPSGRISRLKIVGSKKSIIVGKELEIRRWLSRSHLYSSAF
                     VVKVHRDKLGEIDRFVLYGSGWGHGVGLCQIGAAVMASKGFTAGEILNHYFPGTEIQR
                     LY"
     gene            3010..3729
                     /locus_tag="PK125_12610"
     CDS             3010..3729
                     /locus_tag="PK125_12610"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HPB38987.1"
                     /translation="MKKRRFKTAFILGAGLGTRLRPLTEHCPKPLLNMNGRPIITYAM
                     DHLIGIGVDRFIVNTHHCPEVYSRVFAERQWHGVPIFFRHEPVLLETAGGLKNIEDLL
                     TDDEAIICYNGDVMSSIPLQRLIKFHEKNRPEAAIGLRSSGGLLNVNINNHGEIVDLR
                     HTLGIKGMGSYLFTGIYTVETSLLYFIEAGKIESIIPVFLRRLVAKPGSVMGIVMDEG
                     DWHDIGSIEAYEGLKAHMESR"
     gene            3726..4721
                     /locus_tag="PK125_12615"
     CDS             3726..4721
                     /locus_tag="PK125_12615"
                     /inference="COORDINATES: protein motif:HMM:NF013775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HPB38988.1"
                     /translation="MTTSKTLIKFAKEVLGLAHDVEMKLTPLGGRGSDRIFYRLTWDS
                     TESAILIHYNSIRIENTYYGDIATFLRTINVPVPALICQDSNQCIMVMEDLGNTDLWS
                     LRNEPWDIRQEFYKKTLTVIHVLHTFPIENFPVAQVKLMEPFGPELYKWEQDYFRDNF
                     VIEVCKIKLQPAFSQQLDKELQSLADRIHASSQSLVHRDFQSQNVMIRYGEPYLIDFQ
                     GMRIGSPFYDLGSLLCDPYVQFSEEEIDKLLMFYFKLSESDLIWSEFQKAFWEASTQR
                     LIQALGAFGFLGHKKGLAAFLDYIPAGLANLQHAVSHIPSLPHLQKLLKRCQARI"
BASE COUNT         1294 a         1087 c         1120 g         1321 t
ORIGIN      
        1 taagaccttt gcataaccca tgacctctgc ctctgggagg attttcttct gttttcactc
       61 cctacggtcg gattttcagg gtttggtggt tgtcatatat agtctctatt ttcttctttt
      121 tcattattct cgtggtcttt gaacgctccc cttcctatgt ctcgatcatc cgtgctgcct
      181 ttttcaggtt aaaggccatg gcaatcaaga ggaactccat gttccccttt ttaaggccga
      241 gatagcgcat gcgggcaaac ccatagtgtt tcttcaaagt ccctattccc tgttccaccc
      301 ggtaacggaa ggcgctgatg attctgttga taaaacgctg gaagaaggta agcggttttc
      361 ccctggctgc cttatgcatg attgcatctt catagccgcg ggcagcaaga aggtcgcggt
      421 tcttctggct tgcgtatccc ttgtcagcac agacggggga tgtatcgggt aaaaagaggt
      481 tttccataag gttctcaaat tccgtggtat ccgcaacatg ggcaggggtg acatgaccgt
      541 ttaagataaa cccttctttg gtatccacca ccacatgagt cttatatccg tagtagggtt
      601 tcttcccttt cctgacccag gtggcatcat gatcatcgga gtaagtaatc tggggaggag
      661 gggtatccgt ttcttcttca ctgcgatctt cggccatgac ctcgatgacc tttctcggtc
      721 tccgggacga ttccacaatg gtggcatcga tgatggcccc ttccttaacc agaagtccat
      781 gggattcaat ctgtcggttg atctctccaa agagcttttc atagatgtcc aactccatac
      841 atgtattcct gaagcgacag atggtgctgt gatcaggtaa cgtgcttccc agggaaaaac
      901 cgctgaacct aatgaaggag atacgatcag agagggcatc ctccaatccg ggatcgctta
      961 aaccatacca tctctggaga agtaagagct tgaacatggg aacaggagga taggcaggtc
     1021 tgccatcagc actggcaact ttccggtatt tcttcttcag aatcttctct atgggtttcc
     1081 agtcaatgag acgattcaca tcatcaagga agttcttccg ggattgcttc cggatctcga
     1141 caaagtaatc ggcaaaggtg ggggtggtaa atgtgtcttt caaagatacc tccttcatgt
     1201 tgtcagaatg aggtatatta ccatatatat catatagtta caagttattt caggggaaaa
     1261 taccgtgcaa aggtctcgaa gtttagttga cgctttgctt tgtggattgt atgattatgt
     1321 tgcactgtct ttgaagatac aatctacagt agtgaaaaac ttgatagact ataaagagaa
     1381 cctcttggca cctttctctt gcaaccctct gacccacaca tttaccgaac cctccattac
     1441 tgttggcatc atggaccacc ggaaagaagt cgcaggttgt tttgagggtt attatggtgt
     1501 ggaaggtgtt ggtcaattct ccggaaattt tactgcaagg gacaaaaacg gggcaatcgt
     1561 tctatctgat gccaatggca ctgaaataat ccacggtcta aagatcagat gtacggcaca
     1621 acctgactcg ctattccata tatcagaggt gactatcggg atagactttc actgggaaag
     1681 aaaagaagag caggtttttc agggtaacct catcctggcg ttgcgtaaag atggaatgat
     1741 tgccgtcatc aacgaaatca atcttgagga ttatctgaaa agtgtcattt cctccgagat
     1801 gaatagcagg gctcccaggg aatttctcaa ggctcatgca atactttcca gaagctggct
     1861 catggcagcg ttgaacagaa aaaaagtacc acatcaaccc cgtgaaggag caacaaaaat
     1921 taccgaacat gagattgtcc gctggtatga cagggaagat catgacctct atgatgtctg
     1981 tgcagatgat cactgccaga gataccaggg gatcacaaag atcatatcca aagaggctat
     2041 agatgctgta cgccagacgc atggccaggt catcacgtcc ggggaaacca tctgcgatgc
     2101 cagatactcc aaggcatgtg gcggattaac agaaaattat ggtacagcat gggacgatag
     2161 agaggtccat tatctgacaa gcatatcaga ttcgtcggtt cctcaccgcg caataagaag
     2221 tgaggaagaa gccgagcact ggataagctc gcggccagtg gtttactgca acacgaatga
     2281 tacggacttt cttgaaaatg tgctacggga ttttgaccgg gagacgcgcg atttctttcg
     2341 atggaaaata gaatatgcac gggaagagct tgagatgatt cttcagaaaa aatccggttt
     2401 tgatttcggg acacttcaga atatcgtgcc gcttcaccgt gggccgtcag ggcgaatctc
     2461 aaggttaaaa atagtgggtt caaagaaaag tattatcgtt ggaaaagagc ttgagattcg
     2521 gcgatggctc tcaaggagtc atctctatag cagtgcgttt gttgtgaaag tacatcgtga
     2581 caaacttgga gagattgatc gatttgttct ttatggatcc ggatggggac atggcgtggg
     2641 cctctgtcag atcggtgcgg ctgtcatggc aagcaaagga tttacagccg gggagattct
     2701 caatcactac tttcccggca cggagataca gagattatat tgatgaattt acctgaatct
     2761 cattatcacc tcgtgcccac tcgtccttac ttcttctggt cacgttcgag cccacagagc
     2821 acgctgagaa atccttttat cttcgacctg attttaagag aaatcgaacc tgagattcac
     2881 catgaattcc ccagtctcga attcatggta aactttggac aaaaaactcc gtaccttctg
     2941 cgcgcacttg caggacgggc aagagacaat gggttgactt ttcgcaggta cacggtgggt
     3001 tgggtttaat tgaaaaaacg aagatttaaa actgcattta ttttaggagc aggattggga
     3061 acacggctcc gtcctttgac tgaacattgc cccaagcctc ttctcaacat gaatggtcgc
     3121 cccataataa cgtatgccat ggaccatctc atcggaattg gggtggatcg tttcatcgtc
     3181 aatacacacc attgtccaga ggtatatagt agggtgttcg cagagagaca atggcatggt
     3241 gttcccattt tcttccgtca tgagccggtc cttctcgaaa ctgccggtgg gttaaaaaac
     3301 atagaagatc tcctgactga tgatgaagcg atcatctgtt ataacggtga tgtcatgagc
     3361 agcattcccc tgcaaaggct tatcaagttt cacgagaaga acaggcctga agcagctatt
     3421 ggcctcagaa gcagcggtgg ccttctcaat gtgaatatca acaaccatgg tgagatagtc
     3481 gatttgcgcc ataccctcgg cattaaaggg atggggagtt accttttcac tggcatctat
     3541 accgttgaaa cgtcccttct ctattttatc gaggcaggaa aaatagaatc tatcattcca
     3601 gtttttctga gacgtctggt tgctaagcct ggatcagtaa tgggtatcgt catggacgaa
     3661 ggtgactggc acgatatcgg atccatcgaa gcctatgagg gtctaaaggc acatatggag
     3721 tcccgatgac taccagcaaa acattgatca agtttgcaaa ggaagttctg gggcttgcac
     3781 atgatgtcga aatgaagctt actcctctcg gcggtcgtgg atcagaccgg atattctaca
     3841 gactcacctg ggattctaca gaatctgcaa tccttattca ttacaattca atacgcattg
     3901 agaacaccta ctatggagat atcgctactt tcctcagaac tattaacgtc cctgttcctg
     3961 cactgatctg tcaggattct aaccaatgta tcatggtcat ggaagatctc ggaaacactg
     4021 atctctggtc gctaagaaac gagccatggg acattcgtca agaattttat aagaagactt
     4081 tgactgtcat tcatgtgctt cacacctttc caatagaaaa ttttcccgtg gcacaggtga
     4141 aactcatgga gccttttgga cccgagctct ataagtggga acaagattat ttcagggaca
     4201 actttgtgat tgaagtctgc aaaataaagc ttcaaccagc cttttcacaa cagctcgata
     4261 aagagcttca gtccctcgct gatcgcattc atgcatcgag tcaatccctt gttcaccgtg
     4321 attttcagtc ccagaatgtc atgataagat acggagagcc ctatcttatc gatttccagg
     4381 ggatgcgtat agggagtcct ttttacgatt tgggatccct tctttgtgat ccctatgtgc
     4441 agttttcaga agaagagatt gacaagctat taatgtttta cttcaagtta tcagaaagtg
     4501 atctgatttg gtctgaattt cagaaggcat tctgggaagc ttcaacgcag cgacttatcc
     4561 aggccctcgg tgctttcggt tttttgggac acaaaaaagg gcttgcagcc tttctcgatt
     4621 atattcctgc cggcctggcc aatcttcagc atgcagtatc ccacatacca tccctgccac
     4681 acctccaaaa gcttctaaag agatgccagg cacgcatctg aacttcaaaa tggatcagga
     4741 ttatcccaaa tcacgcaata ccacttactt tatgtgacat aacccgataa catttcgcca
     4801 tattttaacc aatgcgccat ta
//
LOCUS       DAOOMC010000166         4737 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdus sp. isolate AMR_MDS_2685
            NODE_21719_length_4737_cov_5.157625, whole genome shotgun sequence.
ACCESSION   DAOOMC010000166 DAOOMC010000000
VERSION     DAOOMC010000166.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864760
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdus sp. (wastewater metagenome)
  ORGANISM  Syntrophorhabdus sp.
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae; Syntrophorhabdus.
REFERENCE   1  (bases 1 to 4737)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4737)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,729
            Genes (coding)                    :: 2,708
            CDSs (with protein)               :: 2,708
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 7 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4737
                     /organism="Syntrophorhabdus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21719_length_4737_cov_5.157625"
                     /isolate="AMR_MDS_2685"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2699756"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..466
                     /locus_tag="PK125_12620"
     CDS             <1..466
                     /locus_tag="PK125_12620"
                     /inference="COORDINATES: protein motif:HMM:NF012652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HPB38989.1"
                     /translation="LTGHVVLSSLHTNDAAGAVTRLIEMGIEPFLAASAVSCVIGQRL
                     LRKICGECKEQYNPSPAVIKSLNIPDDAVLYRGKGCPSCKYTGYKGRTGVFEVLVIDD
                     SVKDLVISKASSDMIKKAAVENGMTVMHEDAITKVLLGITTLEEALDVTRIE"
     gene            519..1742
                     /locus_tag="PK125_12625"
     CDS             519..1742
                     /locus_tag="PK125_12625"
                     /inference="COORDINATES: protein motif:HMM:NF012694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HPB38990.1"
                     /translation="MGKFTYKARDEKGSLVTGIIEGDNRRAAYAQIDALGLFPVTVSE
                     TREEIFSIESFLTALKPVRYDDIIFFTRQLQTVIRAGIPILSGLRALEEQTSNVKLRG
                     AIKEMVQDIDKGASLSDAMMKQKKLFPDLYTSMVKAGEIGGSLEEVLERLATLLEFQL
                     KTKEMLKSAIRYPIFVVTTLLIAFLVLINFVVPRFAVLFRGAKMDLPLPTRMLLLTND
                     IFQNYAIYLGISIFILIGGFLLYKRTDAGTLEIDRLKLRAPLIGPIILKICMSRFANM
                     FENLIKVGIPVVRTLEVVSRTVGNEYIAQKIIEISGKIERGLGISRPLREAEIFPPLI
                     IHLISTGEQTGSLEDMLKEVSTHYDREITYSINRLSTWIEPLLTAGLSLMVLFLALAI
                     FLPWWNMMGALRGGG"
     gene            1739..2785
                     /locus_tag="PK125_12630"
     CDS             1739..2785
                     /locus_tag="PK125_12630"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HPB38991.1"
                     /translation="MKTFFKDPKNLRYRLSSVIPGVAFLTSILTAATMTNLKMAHNQY
                     FIWLIALAVFSALCSLALVYAMTSPLKDLLIKAEDIVHFEKVKSDRGLMIEVYQLIEK
                     LIELAKSKENGGRKEQAQFIEDIERLDYLLPLGYMSLMVAHEVRNPLNTITGMNELLK
                     NRLEDPQLLKYVQVSLDAAKKIDIFTKDLLDFTDDVVHTEGFDINEVIDESYRALRYE
                     FKDVTCELQKGELPRFVGDRNKIYQAIHNILKNAFDFERDGGYVKVKSTCDQSFVISI
                     YNKNSQIAPEIIDTIFKPFFSKKKGGKGIGLFISMRNVKIHGGNITVTSGDEGTTFII
                     TLPIQDHTGKEEMI"
     gene            2782..4134
                     /locus_tag="PK125_12635"
     CDS             2782..4134
                     /locus_tag="PK125_12635"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HPB38992.1"
                     /translation="MIEKIVIVEDDAGVRFFLEEALKDEGYKIMAFESYEEASSSINK
                     ETNLIIMDIKLPGVDGLTAIEDIKKKSDAPILIITAYGTKKNAMEAIQRGATDFFVKP
                     IALDELKVMVKRVLGTQMLKKELEVSKEEELGQSIFHGVVGKSEVMKEIFRNIEKFAD
                     KDITVLITGETGAGKEAVGRLIHDLSKRSKDFVVVNCASIPDNLLESELFGYEKGAFT
                     GAVQSKQGKFELANGGTIVLDEIGEMSPYLQAKLLRVIEAKEIERLGDIKKRKIDVRI
                     LATTNRALEVEIKEGRFREDLYYRLAQIHLIIPPLRERREDILVLTDKLLLDISKDSG
                     NLVSIADDAKRLLLNYPWPGNVRELINVIKRAAIMCDNNRITLDDLPLHLRGEQPLAS
                     ISYSDKSLDEAISELEKRMIIDALKKTRGLQAKAAKLLGISERSIWYRIKKYSIDVNV
                     "
     gene            4406..>4737
                     /locus_tag="PK125_12640"
     CDS             4406..>4737
                     /locus_tag="PK125_12640"
                     /inference="COORDINATES: protein motif:HMM:NF019575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HPB38993.1"
                     /translation="MDITNKDSAIRYRRQKGFTLVELIIIIVILGILAAVAIPKYIDM
                     KAEASDSTARVILGGLRDAATLVYVNRIVSSSTTPIDMTAVLGQAQISGYDTYSISAD
                     TLTLQISG"
BASE COUNT         1464 a          941 c         1065 g         1267 t
ORIGIN      
        1 cctcacaggg catgttgttc tctcgagcct ccatacgaat gacgcagcag gcgctgttac
       61 acgacttatc gagatgggga tagagccttt tcttgcagcc tctgccgttt cctgcgttat
      121 tggtcaaaga ctccttcgca aaatatgcgg ggagtgcaaa gagcagtaca acccatcgcc
      181 tgctgttatt aaaagcctca atatacccga tgatgctgtt ctttatagag gtaaaggatg
      241 tccctcctgc aaatataccg gatataaagg gagaacaggc gtttttgagg tcctcgttat
      301 tgacgactca gtcaaggacc tcgttattag caaggcttcg agcgatatga ttaaaaaggc
      361 tgctgtagaa aacggaatga ctgttatgca tgaggatgcc attacaaagg tgcttctggg
      421 cataaccacc ctggaagaag ccttggatgt aaccaggata gaatagtgtg cagcgatgaa
      481 tgtgatagaa aaccggaacc caacgaagga aaggatggat gggaaagttt acctacaagg
      541 cccgagatga aaaaggttct ctcgttaccg ggatcataga aggtgacaac aggcgggctg
      601 cttatgcgca gattgatgca ttgggtctat ttcctgtcac agtatcagaa acaagggaag
      661 agatattctc cattgaaagc tttttgacag ccctcaagcc tgttcgttat gatgacatca
      721 ttttttttac ccgtcaatta caaacagtta tacgtgcagg catccccata ttgtctggcc
      781 ttcgtgccct cgaagagcag acatccaatg taaagctcag gggagcaatc aaagagatgg
      841 tacaggacat agacaaaggg gccagtcttt ctgacgccat gatgaaacag aagaagctct
      901 ttccggacct ctacaccagc atggtaaagg ctggggaaat agggggatct cttgaagagg
      961 ttctggagcg ccttgcaacc cttctcgaat ttcaattaaa aacaaaggag atgctcaagt
     1021 ctgccatcag atatccaatt tttgtggtga caactttgtt gatagctttt ctcgttctca
     1081 tcaattttgt tgtaccacgg tttgcagttc tttttcgggg tgcaaagatg gatcttcccc
     1141 ttccaacacg aatgcttctt cttacaaatg acatcttcca gaattatgcc atatatctcg
     1201 gcatctccat ttttattttg attggtggtt ttttgctcta caaaagaacc gatgcgggaa
     1261 cccttgaaat cgacagactg aaattaaggg ccccccttat aggaccaatt atcttaaaaa
     1321 tatgtatgag cagatttgca aacatgtttg aaaaccttat caaggtaggt atacctgttg
     1381 taaggaccct tgaggtggtt tcccgaacag tggggaatga atatatcgcc cagaagatca
     1441 ttgagataag cggaaagata gaaagaggct tgggtatctc cagacctttg cgggaggccg
     1501 agatatttcc gccactcatt atacacctca tttcaaccgg ggaacaaacc ggttcccttg
     1561 aggatatgct gaaagaggta tctacccatt acgacaggga aataacatat tcgataaaca
     1621 ggctttcaac gtggattgag cccctcctga ctgcaggcct ttccttgatg gtattatttc
     1681 ttgccctggc catattcttg ccatggtgga acatgatggg ggctcttagg ggtggtggat
     1741 gaaaactttt tttaaagatc caaaaaattt aagatacagg ctcagcagtg tcataccagg
     1801 cgttgccttt ctcacgagca ttttgactgc agccacaatg acaaatctga aaatggcgca
     1861 caaccaatat tttatatggc ttatagccct ggccgttttc tcggccctgt gttctctggc
     1921 tcttgtatat gctatgacat ctcctctcaa ggatcttctc ataaaggctg aagatatagt
     1981 tcattttgaa aaagtgaaat cagaccgggg gctcatgata gaggtctacc agctgataga
     2041 aaaacttata gaattggcga aatcaaaaga gaatggaggc agaaaagaac aggctcaatt
     2101 catcgaagat atagagcgcc ttgactacct ccttcccctt ggctacatgt ccctcatggt
     2161 ggctcatgaa gtgcggaatc cccttaacac cataacaggg atgaatgagc ttttaaaaaa
     2221 cagacttgaa gacccacagt tgttaaagta cgttcaggtc tctctcgatg ccgcaaagaa
     2281 gatcgacatt tttaccaaag atctgctgga cttcacagat gatgtagttc atacagaagg
     2341 ttttgacata aatgaggtaa tagacgaatc atacagagcc cttcgctatg agtttaagga
     2401 tgtaacctgc gaacttcaga aaggagagtt gcccagattt gttggcgatc gcaacaagat
     2461 ataccaggct attcataata ttctcaaaaa tgcttttgac tttgaacgcg atggcgggta
     2521 tgttaaggta aaaagcacct gtgaccaatc atttgttatt tcaatatata ataaaaattc
     2581 tcagattgcg ccagaaatta tcgacacaat ctttaagcct ttcttctcaa aaaagaaggg
     2641 tggcaaaggt atagggctct tcatcagtat gagaaacgta aaaatccacg gtggaaatat
     2701 cacggttaca agcggtgacg aaggaacaac gtttataatc acccttccca ttcaagacca
     2761 cacaggcaaa gaggaaatga tatgattgaa aaaattgtaa ttgttgaaga tgatgcaggc
     2821 gtgaggtttt tccttgaaga ggcactgaaa gacgaaggtt ataaaataat ggcttttgag
     2881 tcctatgagg aggcttcatc ctcaataaac aaagaaacaa atcttattat aatggatatc
     2941 aaactccctg gagtagatgg ccttactgca attgaagata tcaaaaagaa atctgatgcc
     3001 ccaatcctca ttattactgc ttatggcact aagaaaaatg ctatggaagc aatccaacga
     3061 ggtgcaacag atttttttgt aaaaccaatt gcccttgacg aacttaaagt aatggttaaa
     3121 agagttctgg gaacccagat gctgaagaaa gagttagaag tatctaaaga ggaagagctg
     3181 ggacagtcga tattccatgg agttgtgggc aaatcagaag taatgaagga aatcttcaga
     3241 aatattgaaa agtttgcaga caaagatatt actgttctca tcacgggaga aaccggtgca
     3301 ggaaaggagg ccgtggggcg ccttatccac gatctctcaa agagaagtaa agattttgtg
     3361 gttgtaaact gcgcttcaat tcctgacaac cttcttgaaa gtgagctttt cggttacgaa
     3421 aagggggcct ttacaggggc ggttcagtca aagcagggta aatttgaact ggcgaatggt
     3481 ggcacaatcg tccttgatga aattggagaa atgagtccat acctccaggc aaagctttta
     3541 cgcgttatag aagcaaaaga aatcgaacgt ttaggggata taaaaaaacg caagattgac
     3601 gtacggatat tggcaacaac aaacagggct ctggaggttg aaattaagga agggagattt
     3661 cgagaggacc tgtattacag gcttgcacag attcatctca taatccctcc tctgagggaa
     3721 aggagggagg atattctggt ccttactgat aagcttcttc tcgacatatc gaaagactcc
     3781 gggaatcttg tgagtatagc agatgacgcc aagcggttgc ttctcaatta cccctggcca
     3841 ggtaacgtaa gggagctcat taatgtcata aaaagagctg ctattatgtg cgacaataac
     3901 cgtattaccc ttgatgatct gcccttacac cttcgagggg aacagcctct tgcgagcata
     3961 tcatattcag acaaatccct tgatgaagca atctctgaac ttgagaagag aatgattatt
     4021 gatgccctga aaaaaacaag gggccttcag gctaaggcgg caaaattact tggcataagc
     4081 gaacgaagca tatggtaccg gatcaaaaag tacagtatcg acgtgaatgt ctgatccgcc
     4141 tacaattatt gtagaaaaac cacaatattt gtaggcggaa tatgtattat ctgacagatt
     4201 ttcaggctgt tacaatagtt ttttcatgaa aaacttttga ggtaactatt tttttataac
     4261 tctttggggc tggaaaacct caacgccgca cattaatatt tagtaattta cgaaatgctg
     4321 caactctcgt gtagataaaa cttcaataca taacttttgt ctgatatgcc aatctggtat
     4381 agggattgca atgaggctat gcataatgga cattacaaac aaagattctg ccataagata
     4441 tcgtcgtcaa aagggtttta cacttgtcga gttgataatt attattgtta ttcttggtat
     4501 tcttgctgct gtagcaatcc cgaaatacat tgatatgaaa gcagaagcat cagactcgac
     4561 ggcaagagta atcttaggtg gtctacgtga tgctgcaaca ctggtatatg tcaatagaat
     4621 tgtaagctca tcaaccacac ccattgatat gacggctgtt ttaggccaag ctcaaatttc
     4681 tggatacgac acctattcta tatcagcaga cacactaact ctccaaatat cggggca
//
LOCUS       DAOOMC010000167         4688 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdus sp. isolate AMR_MDS_2685
            NODE_22015_length_4688_cov_6.638895, whole genome shotgun sequence.
ACCESSION   DAOOMC010000167 DAOOMC010000000
VERSION     DAOOMC010000167.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864760
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdus sp. (wastewater metagenome)
  ORGANISM  Syntrophorhabdus sp.
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae; Syntrophorhabdus.
REFERENCE   1  (bases 1 to 4688)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4688)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,729
            Genes (coding)                    :: 2,708
            CDSs (with protein)               :: 2,708
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 7 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4688
                     /organism="Syntrophorhabdus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22015_length_4688_cov_6.638895"
                     /isolate="AMR_MDS_2685"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2699756"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..489
                     /locus_tag="PK125_12645"
     CDS             <1..489
                     /locus_tag="PK125_12645"
                     /inference="COORDINATES: protein motif:HMM:TIGR00530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HPB38994.1"
                     /translation="SALDIFSLLNSLPVQFKWIAKRELFYIPFLGWAMKRAGYISLDR
                     KHPREAIKAMDDAAKKIREGTNIIIFPEGTRSKDGHLLPFKKGGFSLALRAKVPIVPV
                     GISGSNRLQPKGSFIPNQKGVIYLRIGKPVETAQGSRSAKTEIMMTVRQAIEGLMTEK
                     EA"
     gene            526..2532
                     /locus_tag="PK125_12650"
     CDS             526..2532
                     /locus_tag="PK125_12650"
                     /inference="COORDINATES: protein motif:HMM:NF013727.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK 4TM domain-containing
                     protein"
                     /protein_id="HPB38995.1"
                     /translation="MLGVGLLGLFLFLLVSLISYHPFDPSLNALASGGAKNWCGKVGS
                     YISDILIQLFGMMSFILPAVSLLFGLFFVRKKDPPHILLLTSGIVLLFLSLSAILQIS
                     IGQIHLRGVGMPFSGFLGSFFGKMFLKFFNYFGSYLILLILLLISAFLIVQAPLLSMF
                     EARIERRKPLERRKEIKVTEEAPKEEKKVEKKPVQEAFDFFNEIGPYKLPSVSLLDTT
                     EKKEVKIDKESIQANASILEKKLKDYGIDGKVVEVRPGPVITMYEFQPAAGIKVSRIS
                     NLADDLAMALSAVSIRIIAPIPGKDVVGIEIPNKIRQTVYFKEIIESPQFAASNSFLS
                     LALGKTIAGEPFVADLTKMPHLLVAGSTGSGKSVSLNSMICSMLYKATPINVKFLMID
                     LKMLELSFYDGIPHLLLPVVTNAKNAKASLRWLIDEMERRYKMMAEKGVRNIDKYNQK
                     MVKGNMEAIPYIVVVIDELADLMMVSTKEVEEYIARLAQMARASGIHLILATQRPSVD
                     VLTGIIKANFPARISCQVSSKVDSRTILDTNGAESLLGYGDMLFLSPGYGRLQRLHGP
                     FVSESEIKRIVEFLKQQGTPVYHQEILEEREEEGAGEENMDDEKYQEAVEFVMERGEA
                     SISMVQRRFRIGYNRAARIVERMEAEGIVGPSDGVKPREVLKRK"
     gene            2529..3260
                     /locus_tag="PK125_12655"
     CDS             2529..3260
                     /locus_tag="PK125_12655"
                     /inference="COORDINATES: protein motif:HMM:NF015510.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA];
                     GO_process: GO:0044874 - lipoprotein localization to outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein carrier protein LolA"
                     /protein_id="HPB38996.1"
                     /translation="MSDSIECLAITMRREQKKCITCIFFIVMIAICFCVGAGYAAGPH
                     PRTPNSEYQTVLHQSSFDEIKKAYAEIKTLEAQFKQRIFISSLKKERDSKGEFFFKRQ
                     KGFLWRYKTPKVQYFLYDGKFIWQEEDDKPYVAKNKIDKEKTGGTFFDLIEDIAKIDE
                     LFTLKEQKIEENFEVLQLIPKKEGTVISAKIWIDKQKLIKKIEIHEFTGNVNTIEFSS
                     IKVNQSLDESKFVFKSDGKKEIVER"
     gene            3260..4048
                     /gene="recO"
                     /locus_tag="PK125_12660"
     CDS             3260..4048
                     /gene="recO"
                     /locus_tag="PK125_12660"
                     /inference="COORDINATES: protein motif:HMM:TIGR00613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HPB38997.1"
                     /translation="MPLHKDEGIVLFKRAYGESDKIIRLFTLNSGKVAAIAKGANKSQ
                     KRFMNTLEPFNHISLEYFEKHGKGMVRLENAYVIETNEGIEKSLKRACVAGFFTEFVD
                     RLTRDREKHTELFYMLKDVLATIKQRDLTYSDVLYHELNMLQILGFEPNFQTCVYCGK
                     AMDEEEKICFSNQRGGTLCNRCARFIAHKRYDQGVIAQLASMRGKKREQLSCEPVGNY
                     SPFNNGNGKDVIFERDARELLEGFVSFHLEVEFKSYRILKSIMG"
     gene            complement(4114..4371)
                     /locus_tag="PK125_12665"
     CDS             complement(4114..4371)
                     /locus_tag="PK125_12665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB38998.1"
                     /translation="MAVVPGFGSVISSPASTDIVMEEGDNLGMGLVFGLFNATINLGF
                     AITPFLGKFIMDTQGIHSVFYFAGLIGIAGVFISYCSLKPR"
BASE COUNT         1468 a          934 c         1072 g         1214 t
ORIGIN      
        1 agtgcactcg atattttttc gctcctcaat agtctccctg tccagttcaa atggatagca
       61 aagagagaac ttttttatat ccctttttta ggatgggcaa tgaagagggc aggatacata
      121 agcctcgata gaaaacatcc ccgggaagcc attaaggcaa tggatgatgc ggcaaaaaag
      181 ataagggaag gaaccaatat cattatcttt cctgaaggaa caagaagcaa ggatggacac
      241 cttctccctt tcaaaaaagg ggggttttct cttgcgctcc gtgcaaaggt tcctatagtg
      301 ccagtgggga tatccggatc aaacaggcta cagccaaaag gtagtttcat tccgaatcaa
      361 aaaggggtaa tatacttaag aatcggcaag ccagtagaaa cagcccaggg cagccgttct
      421 gctaaaactg aaataatgat gacagtcaga caggctattg aaggcttgat gaccgagaaa
      481 gaggcttagg tacaccagaa catgtcgcca gagctgaaga aggagatgtt aggggttgga
      541 ttattgggtc tttttttatt cctccttgta agtcttatct cataccatcc gtttgatcct
      601 tctcttaatg cattggcttc tggtggggca aaaaactggt gtggcaaggt tggttcatat
      661 atatcagata tcctcataca gttattcggc atgatgagct tcatattgcc cgctgtttcc
      721 ctcctcttcg gtttattctt cgtcaggaaa aaagaccccc cccatatact gctccttaca
      781 tcaggtattg ttctgctctt tttgtctcta tctgccatac tccagattag tatcgggcag
      841 attcacctga ggggcgtagg aatgcctttc tcaggtttcc tgggctcgtt ttttggaaaa
      901 atgtttctga aattctttaa ctattttgga agctacctta tcctcttaat tctactttta
      961 atttcagctt ttttgattgt tcaggcgcca ttgctttcaa tgtttgaggc cagaatagag
     1021 agaagaaaac ctcttgaacg gagaaaagag ataaaggtaa ccgaggaagc gccaaaagaa
     1081 gagaagaagg ttgagaaaaa acctgtacag gaggcatttg atttctttaa tgaaatcggg
     1141 ccgtacaaac tcccgtcggt ctctcttctt gatacaacgg aaaagaaaga ggtcaaaatc
     1201 gacaaggaga gcattcaggc caatgcaagt attctggaaa agaagctaaa agactacggt
     1261 atagatggta aggtagtaga agttcgcccc gggcctgtga taaccatgta cgaattccaa
     1321 ccggcagcag gaattaaggt atccaggatt tcgaatcttg ctgacgacct cgccatggcg
     1381 ttgagcgctg tttcgattcg aatcattgcc cctatacctg gtaaggatgt tgttggtatt
     1441 gaaataccaa acaagattag gcagacagtc tatttcaagg agattatcga atcccctcag
     1501 tttgccgcat cgaattcctt cctgagtctg gcccttggga aaaccatcgc aggtgagcca
     1561 ttcgtagctg atctgaccaa gatgccccac cttcttgttg ctggttctac agggtcagga
     1621 aaaagtgttt ccctcaacag tatgatttgc agtatgctct acaaagcaac gccaataaat
     1681 gtcaagtttc tcatgatcga cctgaaaatg ttagagcttt cattctacga tggtataccc
     1741 cacctgcttt tgccggtagt cacgaacgcc aaaaatgcaa aagcctcact caggtggctt
     1801 atagatgaga tggagcgcag atacaaaatg atggctgaga agggtgtcag aaacatcgac
     1861 aaatataacc aaaaaatggt gaaagggaac atggaggcaa tcccttacat tgtagttgtc
     1921 attgacgagc tggctgatct tatgatggtc tccacaaaag aagttgagga gtatattgcc
     1981 aggcttgcgc agatggccag ggcatcaggc atccatctga ttcttgccac acagcgaccg
     2041 tcagtagatg tccttacagg gataattaag gcaaactttc ccgcccggat ttcctgccag
     2101 gtttcttcga aggttgactc gcggacaatc cttgacacta acggtgcaga gagccttctt
     2161 gggtatggcg acatgctttt cttgagcccg ggctatggga gattgcagag acttcacgga
     2221 ccttttgtat cagagagtga gatcaagaga attgtggaat tccttaaaca acaggggacg
     2281 ccggtctacc accaggagat acttgaagaa agggaagaag agggagctgg tgaagaaaac
     2341 atggatgacg aaaaatacca ggaggctgtt gaatttgtca tggaaagagg tgaagcctcc
     2401 attagcatgg tgcagcgaag attcaggatt gggtacaaca gggctgcccg aattgtggag
     2461 cgtatggagg ccgaaggaat cgttggcccc tctgatggtg tgaaaccgag ggaggtattg
     2521 aaaagaaaat gagtgatagc attgaatgtt tagcgattac catgcggcga gaacaaaaaa
     2581 aatgtataac ctgcattttt ttcattgtga tgattgcaat ctgtttttgt gttggggcag
     2641 ggtatgctgc tggaccgcac cccagaactc cgaactcaga ataccaaact gttttacacc
     2701 agtcctcctt cgatgaaata aaaaaggcat atgctgagat caaaaccctt gaggcccaat
     2761 tcaaacaaag aatttttata tcaagcctga agaaagaacg tgactcgaaa ggtgagtttt
     2821 tctttaagag gcaaaagggc ttcttatggc gctacaaaac acccaaggtt caatattttc
     2881 tttatgatgg aaaatttatc tggcaggaag aggatgacaa gccctatgtg gcaaagaata
     2941 aaatcgacaa agaaaagacc ggtggaacat tttttgacct tattgaagat atagcaaaaa
     3001 tcgatgagct cttcacgctc aaggaacaaa agatagagga aaattttgag gtcttgcaac
     3061 ttatacccaa aaaagaaggg actgttattt ctgcaaagat atggatcgat aagcaaaaat
     3121 tgatcaagaa gattgagatt catgaattta ccggtaacgt aaacacaatc gagttttcat
     3181 ccatcaaagt gaatcaatcc ttggatgaaa gcaagtttgt tttcaagtct gacggtaaga
     3241 aggagattgt tgagagataa tgcccctcca caaagatgaa ggaatagttt tgtttaaaag
     3301 agcctacgga gaatcggaca agattatacg acttttcacg cttaactccg ggaaggtggc
     3361 tgctatcgcc aaaggggcaa acaaaagtca aaaaaggttt atgaataccc ttgaaccttt
     3421 caaccacatc tctctggagt attttgaaaa gcacggtaaa ggcatggtcc gcctggaaaa
     3481 cgcgtatgtc atagaaacga atgaaggcat agaaaaaagc ctgaaaaggg catgcgtggc
     3541 tggatttttc acggaatttg tcgataggct gacacgagac agggaaaaac atactgagct
     3601 tttttatatg ctgaaagatg ttctagctac aatcaagcaa cgcgacctca catatagtga
     3661 tgtgctctat cacgaattaa acatgcttca aattctcggt tttgagccaa attttcaaac
     3721 ctgtgtttac tgcggaaaag caatggatga agaagaaaag atttgttttt ccaaccaacg
     3781 tggtggcact ctttgtaatc ggtgtgcccg tttcatagca cacaaaaggt atgatcaggg
     3841 ggttatagca caacttgcat ccatgagagg taaaaaaagg gagcaattat cctgtgaacc
     3901 ggttggcaac tattcacctt tcaacaatgg aaacggaaag gatgttattt ttgagagaga
     3961 cgctcgggaa ttacttgaag gttttgtctc ttttcatctc gaggtagaat tcaaatctta
     4021 ccggattctc aaaagcataa tgggctgact aacatatatt aacatcccct tgtttacacg
     4081 caaacgcttc aattagttta caacttagca acatcacctt ggttttaatg aacagtagga
     4141 gataaaaaca ccggcaattc caattaatcc cgcgaaataa aagacagagt gtattccctg
     4201 agtatccatt ataaattttc caagaaaagg agttattgca aatcccaggt ttattgtagc
     4261 gttgaaaaga ccgaaaacta gccccattcc aaggttatca ccctcctcca tgactatatc
     4321 ggtgcttgca ggtgaagaaa taacacttcc aaacccagga acaacagcca tacataatag
     4381 actccaaaac ccgaaagcaa acggtagaaa ggaaatcaaa atggaagtca aaagcccgcc
     4441 cataagtaca agtactcacc ggtttcgatg atctcctaaa aaacacatgg gttttatgaa
     4501 cgccatcatg cgaactgtcc ctgaaaccat gatggctcca atctgcattt caccggggtc
     4561 acggtcggtc tctatgaaaa ggggaagaaa gattgtaaaa atacttattc caatagccct
     4621 ggtgaagata aaagtgggaa gtcctgcgag aatattattt ttttaataga gtatggcaga
     4681 tccttacc
//
LOCUS       DAOOMC010000168         4622 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdus sp. isolate AMR_MDS_2685
            NODE_22431_length_4622_cov_5.627326, whole genome shotgun sequence.
ACCESSION   DAOOMC010000168 DAOOMC010000000
VERSION     DAOOMC010000168.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864760
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdus sp. (wastewater metagenome)
  ORGANISM  Syntrophorhabdus sp.
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae; Syntrophorhabdus.
REFERENCE   1  (bases 1 to 4622)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4622)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,729
            Genes (coding)                    :: 2,708
            CDSs (with protein)               :: 2,708
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 7 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4622
                     /organism="Syntrophorhabdus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22431_length_4622_cov_5.627326"
                     /isolate="AMR_MDS_2685"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2699756"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..340)
                     /locus_tag="PK125_12670"
     CDS             complement(<1..340)
                     /locus_tag="PK125_12670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB38999.1"
                     /translation="MKKKINEGKGIFNPVDHTDAKVRERKFENQIEMWLDEKGRQEQA
                     GELSPGTTGNYHGYAKNYYHILNDYDVRNIKKGEITTLKDTLSNVSIKARKNILNCLH
                     GFFRWMYERGT"
     gene            complement(337..1074)
                     /locus_tag="PK125_12675"
     CDS             complement(337..1074)
                     /locus_tag="PK125_12675"
                     /inference="COORDINATES: protein motif:HMM:NF012464.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HPB39000.1"
                     /translation="MRTMGYVRVSTDEQAREGISLENQKARIAAYCEYRGFTLTGIIE
                     DAGISGGKNRARPGFIEVLNKVESGEAEALVLYSLERLSRDMLTLLALERLLDEYDIE
                     LHTVEGEVNTSTPDGFVNFAMKAFLGEMERRQVKYRTKKAMEFKKAQGSIVGSVPYGY
                     RRLKDYLVPEEGEQKVIALAKSLYGASYCLSEICRRLTEQGYFTRNGKPFTVEQVKRI
                     LPDYEKTFSHSNTRLSQNIRGFIEAIA"
     gene            1229..1411
                     /locus_tag="PK125_12680"
     CDS             1229..1411
                     /locus_tag="PK125_12680"
                     /inference="COORDINATES: protein motif:HMM:NF024139.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HPB39001.1"
                     /translation="MTEETKRLFTVNEACIYLSISRMSLYRMMERKEITPVKIGNRTL
                     FDKKDLDEFIEKSKKL"
     gene            1444..1629
                     /locus_tag="PK125_12685"
     CDS             1444..1629
                     /locus_tag="PK125_12685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB39002.1"
                     /translation="MKHYRAVFLVLVTLFPLSFFSCASYEAVAIPKLQPEFAVNAKKQ
                     NDVIVAARVFSKDDCRK"
     gene            2195..2767
                     /locus_tag="PK125_12690"
     CDS             2195..2767
                     /locus_tag="PK125_12690"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HPB39003.1"
                     /translation="MADQRHTKKQILDIAENLLRDRGYNGFSYKDISSSLGIRNASVH
                     YHYPTKTDLGVAIIRRATHRFENWAQSLVSRDLGCSEKLEEFCLIFRKFVDRQQQICL
                     GGALETDFKTIPEEMQKETCEFVSFVLQWLENLLEDGKEQGAFNFPGKARDQALMTAA
                     SLQGAVQLVRATTTSSFDAIMKQIARSVHR"
     gene            2825..3610
                     /locus_tag="PK125_12695"
     CDS             2825..3610
                     /locus_tag="PK125_12695"
                     /inference="COORDINATES: protein motif:HMM:NF024246.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HPB39004.1"
                     /translation="MTTEEIKETIKEFALGLGVDAVGFASTGDYRSERSPAIKDLLPN
                     AQSMVVLGYREPDGALNSPIARISMAARLAVMDLAKRNSLIIAKFIENQFAAEAAFVL
                     VSYPMDSQKGLVADVSLRHAAVAAGLGVFGRHNLVIHPVFGTRILFTAILTSLQLSSD
                     PSIQEELCLQCNLCVEECPAGALDKEGFTDVMKCLAVSQPYGVGGLIRYAGKFLGAAP
                     DEKKQLLRNPLFQSLYQASYIGQQYECFKCEAVCPIGRSGKKQ"
     gene            4299..4371
                     /locus_tag="PK125_12700"
     tRNA            4299..4371
                     /locus_tag="PK125_12700"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4332..4334,aa:Thr,seq:tgt)
     gene            4375..4460
                     /locus_tag="PK125_12705"
     tRNA            4375..4460
                     /locus_tag="PK125_12705"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4409..4411,aa:Tyr,seq:gta)
BASE COUNT         1231 a         1052 c         1004 g         1335 t
ORIGIN      
        1 tagtaccgcg ttcatacatc cacctgaaaa agccatgcag gcaattaaga atattctttc
       61 tcgcctttat gctgacattg gataaggtat ccttcaatgt ggtaatctca cctttcttga
      121 tgttccttac atcgtaatcg ttgagtatat ggtagtagtt ctttgcgtat ccgtgatagt
      181 tgcccgtagt ccctggagag agttctcctg cctgttcctg cctgcctttc tcatccagcc
      241 acatctctat ttgattctca aatttccgtt ctctaacttt cgcgtcggta tggtctacgg
      301 gattaaatat acccttcccc tcgttaatct ttttcttcat gctatcgcct caatgaagcc
      361 tctaatgttc tggctaagcc gagtatttga atgggagaag gtcttttcgt agtctggtag
      421 gatccgcttc acctgttcca cggtgaaagg ttttccgttg cgagtaaagt atccctgttc
      481 cgtcaatcgt ctgcatatct ctgacaaaca gtaggatgct ccatatagcg actttgccag
      541 ggcaatgacc ttttgttccc cctcttctgg caccaaataa tccttgagcc ttctgtaacc
      601 atagggaacg gatcccacta tagaaccctg agccttcttg aactccattg ccttcttggt
      661 acggtacttt acttgccgac gctccatttc gcccaggaag gctttcatgg caaagttgac
      721 aaacccgtcg ggggtgctcg tgttcacttc accctctacg gtatgaagct ctatatcata
      781 ctcatcgaga agtctttcaa gggcaagaag cgtaagcata tccctgctca accgttcaag
      841 actgtagagc acaagcgcct ctgcttcccc tgattccact ttgttcaaga cctcaatgaa
      901 gccaggtcga gccctgttct tacccccgct gatccctgca tcttcaataa tgcctgtgag
      961 cgtgaaaccc ctgtactcac agtatgctgc aatccttgcc ttctggttct caagactgat
     1021 accttccctg gcctgttcat cggtgctcac cctcacataa cccattgtcc tcatattatc
     1081 ccctttctat ctccttattt gttatatctg tcgatatcac tctatatcta attataccaa
     1141 ataatatcat aaacgcaaga aaaaagatac aacctgatac atttaggtat tgttattgat
     1201 gtttggatac gctatacttc aaatagttat gactgaggaa acaaaaaggc ttttcactgt
     1261 aaatgaagct tgcatctatc tgagcatctc caggatgtca ctttaccgta tgatggaacg
     1321 gaaagagata accccggtaa agataggcaa tagaacgctg tttgataaaa aggaccttga
     1381 cgagttcata gagaaatcta aaaaattata atattcttaa ataaaacaag gggggggtat
     1441 ccaatgaaac attatcgggc agtttttctt gtgttggtaa cactttttcc tttgtctttt
     1501 ttcagttgtg cgagttatga agccgtagct attcctaaac tgcaaccaga atttgctgtt
     1561 aatgcaaaga agcaaaacga tgtcattgta gctgccagag ttttcagtaa agatgattgc
     1621 cgtaagtagg ggtcactaca gaattcggat tataaagcac gttaagcatt ttgcaatggt
     1681 tgtaatggcc tgaaattagt cattatagtt ctctttggtt atatttcgtg caattaaaaa
     1741 tcgggggaac gagggcatca ctaacattga atatagaacc tactttatac tgagtcgata
     1801 acgattgccg tgaactacac ctgcctatac gtagcaactt cacctactat taaataaaga
     1861 gtagaagtcc gtcgagaatt tattaccaga aaaaagaggc gaaagcatca ctttttcgtg
     1921 acatttccgt cgagcacatt ccttgccgct tcgcccaatt cttgcctgaa aatcggtttc
     1981 ttgacgagtt ccctgattcc tatcaccatc acagattcac tgtctacatc gtcgctattc
     2041 tcttccagca gttaccgggt ggtcctgcag tacgcggttc gtttaacccg attccgatgg
     2101 ctctatccac tcttttcacc gagaaattgt cttgctttac tttctaccta ttagtaggta
     2161 tattttggta agcaaacgtt cccgtgaggc ttcaatggcg gatcaaaggc atacaaaaaa
     2221 acaaatactc gatatcgcgg agaatctgct gcgcgatcgg ggctacaatg gatttagcta
     2281 caaggatatc tccagttcac ttggaatcag gaatgcgtca gttcattatc actatccaac
     2341 gaaaaccgat ctgggcgtcg ccataatccg tcgcgcgaca caccgcttcg agaattgggc
     2401 acaatcgctg gtaagccggg atctcggatg ctctgagaaa cttgaagagt tctgcctcat
     2461 cttcaggaaa ttcgtggacc gacagcagca aatctgcctt ggaggggcac ttgaaacgga
     2521 tttcaagaca attccggagg agatgcagaa agaaacatgc gaattcgttt ctttcgtact
     2581 acaatggttg gagaatcttc tggaagacgg caaagaacag ggagcgttca atttccctgg
     2641 caaggcgcgg gaccaggctc ttatgacagc cgccagcctt cagggggccg tacaattggt
     2701 tcgtgcaacc acgacatcca gttttgacgc tattatgaag cagattgccc ggtcagtaca
     2761 ccggtaattt tttggctgct taacatacct attagcaggt aacatattga gtagggggtg
     2821 atttatgact accgaagaga taaaagaaac gattaaggag tttgctcttg gattaggagt
     2881 ggatgctgtc gggtttgcga gcacaggaga ttataggtcg gaaagaagcc cggcgataaa
     2941 agaccttttg ccaaacgccc agtcgatggt tgtactgggc tatcgggaac ctgacggagc
     3001 cttaaacagc cccattgcca ggatttctat ggctgcacga ttggcggtga tggacctggc
     3061 caagagaaac agcctgatca ttgccaagtt catcgaaaat caattcgctg ctgaggcggc
     3121 attcgtcctg gtctcctatc ccatggacag ccaaaagggg ctggtggccg atgtctcact
     3181 ccgtcatgcc gccgtagctg cgggactcgg tgtgttcggc agacacaacc tggttattca
     3241 tcctgtattt ggaactcgaa ttctctttac cgctattttg acaagtttgc agctcagctc
     3301 agatccctcg atccaggagg agttgtgctt acagtgcaat ctttgcgttg aagaatgtcc
     3361 tgccggagca ctcgataagg aaggctttac agacgtaatg aaatgtcttg ccgtatcgca
     3421 accctatggt gtaggtggac tgattcggta tgccggcaaa tttcttggag ccgcccccga
     3481 tgaaaagaaa cagctgctca ggaacccgct gttccaaagt ctttaccagg cgtcttatat
     3541 cgggcagcag tacgaatgtt tcaaatgcga ggccgtctgc cctattggcc ggagtgggaa
     3601 aaagcaataa agaagaatac ttggtcggaa aaattaactg ttaatgtgta taacttcatt
     3661 attaatgtgt atttagtgat gtgcgtgaat gttcttttta taattttata cagaaaagca
     3721 cttatcaata ttcctattga ttgataaaaa accccctacc ctccccctat agtgtcatat
     3781 tcactgtgct catggtgacc atacttttat taagttttag aaaacaataa atacactata
     3841 ttggttaatg ccaactcttg ccaactcttg ccgagtcact tttatagtag ttggcattgg
     3901 taagtattta caattataaa cattatttct tattattgcc aacttgccaa ctatactaat
     3961 aaaacaagat aggtaatttt ttttgtgtgt attctgtggt atactatctt ttgtggtttt
     4021 ggttctaaat tggtgctgtc ctattcgtag actaagcatt gacaacggtg gactcgtttt
     4081 cgattcccgc cgcctccacc aaaaaatact ggtcagcaat tgtcagtgag cggtacgcgg
     4141 caagaaacaa aaaggaaatg aatagagaga atgacggatc agctttcaat aacgatgtgt
     4201 aatgaggttt tttccaaaga ataatattga catacacaca gaaaagttgt aacaattatt
     4261 gcttttcgca agcactttgg attataatgt taaattcggc tggtgtagct caatcggcag
     4321 agcagctgat ttgtaatcag caggttgcgg gttcgagtcc catcaccagc tcatggagag
     4381 gttcccgagc ggccaaaggg ggcagactgt aaatctgttg gcgcagtcct tcggaggttc
     4441 gaaccctccc ctctccacca taaaaagttg caatcagctg tcagccgacc gcaatcaggc
     4501 catttttcgc tgactataaa atgctgactg ctgaaagctg ttaaacagaa ggcgggaata
     4561 gctcagttgg ctagagcatc agccttccaa gctgagggtc gcgggttcga atcccgtttc
     4621 cc
//
LOCUS       DAOOMC010000169         4622 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdus sp. isolate AMR_MDS_2685
            NODE_22433_length_4622_cov_4.987300, whole genome shotgun sequence.
ACCESSION   DAOOMC010000169 DAOOMC010000000
VERSION     DAOOMC010000169.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864760
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdus sp. (wastewater metagenome)
  ORGANISM  Syntrophorhabdus sp.
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae; Syntrophorhabdus.
REFERENCE   1  (bases 1 to 4622)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4622)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,729
            Genes (coding)                    :: 2,708
            CDSs (with protein)               :: 2,708
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 7 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4622
                     /organism="Syntrophorhabdus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22433_length_4622_cov_4.987300"
                     /isolate="AMR_MDS_2685"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2699756"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2474)
                     /gene="leuS"
                     /locus_tag="PK125_12710"
     CDS             complement(<1..2474)
                     /gene="leuS"
                     /locus_tag="PK125_12710"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HPB39005.1"
                     /translation="MQSYNPQAIEEKRQEIWEQERVFRASEDGSREKYYLLEMFPYPS
                     GKIHMGHVRNYSIGDVIARYKRMRGYNVLHPMGWDAFGMPAENAAIDRGIQPATWTYE
                     NIDYMRKQLKRLGFSYDWEREIATCDVDYYRWEQWIFLKMLEKGLVYRKNSPVNYCQK
                     CQTVLANEQVEGGACWRCGETVIQKELTQWFFAITQYADELFEYCDKLPGWPERVLNM
                     QRNWIGKSYGVEVNFPMEDGKPLTIFTTRPDTLFGVTFMVLAPEHPLATTLPKGTPHE
                     QEVLAFVEKTKTQDKSFRAEMAVSKEGVFTGKYAINPLTHTKVPIYIGNFVLMEYGTG
                     AIMSVPAHDQRDFEFAKEYNIPIIVTIMPEKTVLDPATMTEAYEGDGKMVNSGPFNGR
                     QNREAIPSIIDYLEENKLGKRRINFRLRDWGISRQRYWGTPIPIIYCESCGTVPVPYD
                     DLPVVLPVDLAVTMVGKSPLAECADFYQIECPLCKAPAKRETDTMDTFVESSWYFLKY
                     ACPDYNEGPLDSAKVNYWMPVDQYIGGVEHAVLHLLYSRFFNRVLNEFGLVSVREPFE
                     NLLTQGMVIKNGAKMSKSKGNVVDPEYMIKKYGADTARLFCLFASPPEKDLDWNDKGV
                     EGCSRFLQRVWRLIAERINDMRDVEAIFEPLTSDSDAVQLTYLMHKTIKKVTEDIDRF
                     HLNTAIARIMELVNGLYKFLEKDREDEDTKKLTRGAFETLVSLLFPFVPHIAEELWEM
                     LGKDSRMIGKEWPRYNPQYVEEDTVLVVVQVNGKLRDRCEVDRDIDQEQLREIVLTLD
                     NVKRHIEGKTVMKTIVVPNKLVNVVV"
     gene            complement(2519..3724)
                     /locus_tag="PK125_12715"
     CDS             complement(2519..3724)
                     /locus_tag="PK125_12715"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: protein motif:HMM:NF001770.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HPB39006.1"
                     /translation="MGNEIQKVVLAYSGGLDTSIIVKWLLDKYKCEVIAFAADVGQEE
                     ELKGLKEKAIKTGASKIYIDDLREEFARDFVFFALKANAIYEGVYLMGTSIARPLIAK
                     RQIEIARKEKADAVCHGSTGKGNDQVRFELTYYALEPGIKVIAPWREWDFTSRDDLID
                     YAKKHGIPVPVTKKKPYSMDRNMMHISYEGGILEDPWNEPDESMFLTTVSPEKAPDKP
                     TYVEIDFVNGAPVRIDGKTMSPAKIVMFLNKVGGKNGIGRVDMVENRFVGMKSRGVYE
                     TPGCTILHAAHRAMESITMDREVMHIRDGLIPKISELVYYGFWYSPEMNILRALVDES
                     QIGVTGTVRMKLYKGNCIVAGRKSDVSCYRQDFATFDKDVVYDQKDAGGFIRLNSLRL
                     RIRAMLDKK"
     gene            complement(3808..>4622)
                     /gene="argF"
                     /locus_tag="PK125_12720"
     CDS             complement(3808..>4622)
                     /gene="argF"
                     /locus_tag="PK125_12720"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: protein motif:HMM:NF001986.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HPB39007.1"
                     /translation="EHRPLKNKNLAMIFEKASTRTRVSFEVGMRDLGGHPIYMSAGET
                     QIGRGEPIKDTARVLSRYTDAIMIRTYSQNNVDQLAYWASVPVINGLSDLYHPCQILA
                     DLFTISENKGDMTDLKIAYVGDGNNVANSWIEAAILFDLNLSVATPKGYAPDEALLKR
                     AKKSGRFLLTSDPADAIEGADVVNTDVWASMGQESQKEERKIAFDGYMIDTKLLKHAK
                     NNVIVLHCLPAHRNEEIAEDVFEKYSDVIFQQAENRLHAQKALLEWLIKGSS"
BASE COUNT         1145 a         1175 c          959 g         1343 t
ORIGIN      
        1 ttaacaacaa cattcacaag cttattagga accacaatcg ttttcataac cgtttttcct
       61 tcaatgtgcc gtttcacatt gtccagcgta aggacgattt ctcttagctg ttcctggtct
      121 atgtcacgat caacctcgca gcggtccctt agttttccat taacctgaac aaccacgagc
      181 actgtgtctt cctctacgta ttgagggttg tatcggggcc actcctttcc tatcatccgg
      241 gaatccttgc ccaacatctc ccacaattcc tctgcaatat gcgggacaaa gggaaaaaga
      301 aggctaacca gtgtttcaaa agcaccacgg gtgagcttct ttgtgtcctc atcttcacga
      361 tctttttcga ggaatttata aaggccgttc acgagttcca taatcctggc aatggcagtg
      421 ttcaagtgga atctgtcaat atcctcagta actttcttga ttgttttatg catgagatag
      481 gtaagttgaa cagcatcgct gtcactggtt aagggctcaa agattgcctc aacatccctc
      541 atgtcattaa tgcgttctgc aataagccgc cataccctct gtaagaatct ggaacagccc
      601 tcaacacctt tgtcgttcca gtcaaggtct ttttccggag gggatgcaaa caggcaaaaa
      661 agcctggcag tgtctgcccc atacttttta atcatgtact cggggtcaac aacatttcct
      721 tttgatttgc tcatctttgc cccattttta ataaccatgc cctgggttag cagattttca
      781 aaaggttctc ttacagaaac caagccaaat tcattgagca ccctgttgaa aaaccttgaa
      841 taaagaagat ggagcacagc gtgttcaacg ccaccaatat actggtcaac gggcatccag
      901 taattaacct tcgcgctatc aagggggcct tcattataat cagggcatgc atatttcaga
      961 aagtaccagg aagattcaac aaaggtgtcc atggtgtctg tttcccgttt tgcaggtgcc
     1021 ttacacagtg gacattctat ctgatagaag tcagcacatt cagccaacgg tgacttcccc
     1081 accatggtaa ccgctaaatc aacagggaga acaacaggaa ggtcgtcata aggaacaggg
     1141 acagtaccgc agctctcgca gtagatgatg ggaatgggtg ttccccaata acgttggcga
     1201 gaaatccccc agtcacgaag acggaagttt atcctccgct tgcccagttt attttcttcc
     1261 aggtaatcaa taattgatgg gatagcctcc cggttctgtc ttccgttgaa gggaccagaa
     1321 ttgaccatct tcccgtcccc ctcataagcc tctgtcatgg ttgccgggtc aagaaccgtt
     1381 ttttccggca taatggtgac tattataggg atgttatact ctttcgcaaa ctcgaaatcc
     1441 ctctggtcgt gtgcaggcac agacatgatg gcgccggtac catactccat gaggacaaaa
     1501 ttgccgatat atatgggcac ctttgtgtgt gtcaggggat tgatggcata ttttcccgtg
     1561 aagacgcctt ccttgctgac tgccatttca gctctaaaac ttttgtcctg ggtctttgtt
     1621 ttttcaacaa aggcaaggac ctcttgctca tggggggttc ccttgggaag cgtcgttgct
     1681 aaaggatgtt caggtgcaag caccatgaaa gtgactccaa aaagcgtatc aggtcgtgtg
     1741 gtaaagatgg ttaacggttt gccatcttcc ataggaaaat ttacttcaac gccatagctt
     1801 ttccctatcc aattcctctg catattgagg acccgttcag gccacccagg caacttatcg
     1861 caatattcaa aaagttcatc cgcgtactgg gtaatagcga aaaaccactg ggtaagctct
     1921 ttctgtataa ccgtttcacc acagcgccag catgcgcctc cctcaacctg ttcatttgca
     1981 aggactgtct ggcatttctg acagtagttc acaggggagt tttttctgta gacaagcccc
     2041 ttttcaagca tcttgaggaa aatccattgt tcccaacggt agtaatcaac atcgcatgtg
     2101 gctatctccc tttcccagtc atagctgaaa ccaagccgtt taagctgttt cctcatgtag
     2161 tcgatgttct cataggtcca ggttgcaggt tggatacctc gatcaatggc ggcattctct
     2221 gccggcatac caaaggcatc ccaccccata ggatgaagta cattgtagcc ccgcattcgc
     2281 ttgtatcggg ctatcacatc accgatagaa tagttccgca cgtggcccat gtgaatcttg
     2341 cctgatggat agggaaacat ttccagaaga tagtacttct ccctcgaacc gtcttctgag
     2401 gccctgaaga cgcgctcctg ctcccagatt tcttgtcttt tttcttctat agcctggggg
     2461 ttgtacgact gcattctaaa cctcatttaa acctgtaacg cctgacacgc aacaggtatt
     2521 attttttatc gagcattgcc ctgatccgga ggcgcagtga attaaggcgg ataaaaccgc
     2581 ctgcatcttt ttgatcatag acaacatcct tatcaaaggt ggcaaaatct tgccggtagc
     2641 aggatacgtc tgatttgcgt cctgccacaa tacaattgcc tttgtagagc ttcatcctta
     2701 ctgtccctgt aacgcctatc tggctttcgt caaccagggc tctcagtata ttcatttcag
     2761 gagaatacca gaacccgtaa taaacaagtt ctgagatctt gggtatgagc ccgtcacgta
     2821 tgtgcatgac ctctcggtcc atggtgattg actccattgc tctgtgggca gcgtggagaa
     2881 ttgtgcatcc aggagtttca tagacacccc gtgacttcat acctacaaac ctgttttcca
     2941 ccatgtcgac tcttccgata ccattcttgc caccaacttt attaagaaac ataacaattt
     3001 tagcagggga catggttttt ccgtcaatcc ttacaggggc cccattgaca aagtcgattt
     3061 caacgtaggt tggtttgtca ggtgcttttt ccggtgaaac ggtggtaagg aacatactct
     3121 catcgggttc attccacgga tcttcaagaa tgcccccttc ataactaata tgcatcatgt
     3181 tcctgtccat gctgtaaggc ttcttcttgg ttacaggtac cggaatgccg tgtttctttg
     3241 cataatcaat gaggtcatca cgagaagtga aatcccattc cctccatggt gcaataacct
     3301 ttattccggg ttccagggca tagtacgtaa gttcgaaccg aacctgatca ttccccttgc
     3361 ccgtagagcc atgacagacc gcgtcagcct tttcttttct ggcaatttct atctgccttt
     3421 ttgcgatcag tggacgggca atagaggtac ccattagata gacgccttcg tatatcgcat
     3481 ttgccttaag ggcaaaaaaa acaaaatctc gcgcaaactc ttcccgtaaa tcatcaatat
     3541 agatcttgga agcgcctgtc ttaatggcct tttccttgag tccttttagc tcctcttcct
     3601 gccctacatc ggctgcaaaa gcaataacct cacatttgta tttgtcaagg agccacttga
     3661 cgataattga tgtgtcgaga ccgcctgaat aagccagtac cactttctgt atttcattac
     3721 ccatgatata cctccggttt tttagctctc agctcacagc ccaaagcccc taaaaggggc
     3781 attacctttg agcttacagc tcgtcagcta tgagctacct tttatcagcc attccaacag
     3841 cgctttctgg gcatgcaatc tgttttcagc ctgttgaaaa ataacatcgc tgtatttttc
     3901 aaaaacgtcc tctgcgattt cttcgtttct atgggcaggc aagcaatgaa gaactatcac
     3961 attattcttg gcatgtttta gaagcttcgt atctatcatg tatccgtcaa aggctatttt
     4021 cctttcttcc ttctggctct cctgccccat actggcccac acatctgtat tgaccacatc
     4081 tgcaccctct atcgcgtctg ccgggtcact ggttaataaa aatctgccgc tcttttttgc
     4141 tcttttgaga agggcctcgt ccggcgcata gcccttcggt gttgctacag aaagattaag
     4201 atcgaagagg atagcagctt caatccacga attagcaaca ttgtttccat cacccacata
     4261 tgcaattttc agatctgtca tgtccccttt attttctgaa atcgtgaaga gatctgccag
     4321 gatctggcag ggatggtaaa ggtctgaaag gccattaatt acaggaacac tggcccagta
     4381 ggcaagttga tccacattgt tttgtgagta tgtacgaatc atgatagcat ctgtatagcg
     4441 gctcagcaca cgggctgtat cctttatggg ttctcctctg ccgatctgcg tctcaccggc
     4501 actcatgtat atggggtgcc ctccgagatc acgcatccca acttcaaacg agactcttgt
     4561 ccttgtagat gccttctcaa agatcatggc caggttttta ttcttcagtg gcctatgttc
     4621 tt
//
LOCUS       DAOOMC010000170         4594 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdus sp. isolate AMR_MDS_2685
            NODE_22621_length_4594_cov_5.123816, whole genome shotgun sequence.
ACCESSION   DAOOMC010000170 DAOOMC010000000
VERSION     DAOOMC010000170.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864760
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdus sp. (wastewater metagenome)
  ORGANISM  Syntrophorhabdus sp.
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae; Syntrophorhabdus.
REFERENCE   1  (bases 1 to 4594)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4594)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,729
            Genes (coding)                    :: 2,708
            CDSs (with protein)               :: 2,708
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 7 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4594
                     /organism="Syntrophorhabdus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22621_length_4594_cov_5.123816"
                     /isolate="AMR_MDS_2685"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2699756"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            1885..2112
                     /locus_tag="PK125_12725"
     CDS             1885..2112
                     /locus_tag="PK125_12725"
                     /inference="COORDINATES: protein motif:HMM:NF016167.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF433 domain-containing protein"
                     /protein_id="HPB39008.1"
                     /translation="MNWRERITANPLVCHGKACIKGTRIMVSIILDNLADNASEEDIL
                     KSYPSLSPEDIKAAIAYAAELSRERLVAVSL"
     gene            2120..2482
                     /locus_tag="PK125_12730"
     CDS             2120..2482
                     /locus_tag="PK125_12730"
                     /inference="COORDINATES: protein motif:HMM:NF037832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5615 family PIN-like protein"
                     /protein_id="HPB39009.1"
                     /translation="MFFKIDENLPADLAELMVTHGYDVKTVYDQQLQGVEDLILMGRC
                     DEENRILITLDTDFSDITLYPPEKHAGVIVLRLGNQAKKNILDVFRRIIPALEREPIK
                     HHLWIVEETVIRIRGKKE"
     gene            2550..2915
                     /locus_tag="PK125_12735"
     CDS             2550..2915
                     /locus_tag="PK125_12735"
                     /inference="COORDINATES: protein motif:HMM:TIGR02436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HPB39010.1"
                     /translation="MERKDLENRTKAFAVQIITFVTNLPKNKVTDVLGYQLIKSGTSI
                     GANYREARSAESHDDFIHKIGIVEKEASETQYWLELLRESSIGNLDELQSLLQECSEL
                     CAIFTATGKTAKSNRNKKQ"
     gene            complement(2973..3101)
                     /locus_tag="PK125_12740"
     CDS             complement(2973..3101)
                     /locus_tag="PK125_12740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB39011.1"
                     /translation="MAQRESGIAKWNAKAKYTVRHGEREKGKANGATRNVQANAKV"
     gene            3241..4203
                     /locus_tag="PK125_12745"
     CDS             3241..4203
                     /locus_tag="PK125_12745"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HPB39012.1"
                     /translation="MYNFALLGAAGYIAPRHMKAIKDTGNNLVAAMDPHDAVGIMDSY
                     FPGARFFTEFERLDRHAEKLRRRHDDEQRIHCVSITSPNYLHDAHIRFALRVGANAIC
                     EKPLVLNPWNVDPLQELERESGRKINTILQLRHHPAIIVFKEKMQGMPRDKKVDIDLT
                     YITSRGRWYLVSWKGDITKSGGVATNIGIHFFDMLQWVFGSVRENTVHVSEATKAGGY
                     LELERARIRWFLSIDRTDLPQEVLEANKTTYRCITADNEEVEFSDGFTDLHTVSYQKI
                     LAGEGFGLVDVKPSIEIAHDIRNATPIGLKGDYHPALKTSKSEG"
     gene            4295..>4594
                     /locus_tag="PK125_12750"
     CDS             4295..>4594
                     /locus_tag="PK125_12750"
                     /inference="COORDINATES: protein motif:HMM:NF012360.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DapH/DapD/GlmU-related protein"
                     /protein_id="HPB39013.1"
                     /translation="MRNNNNYFVHKSSYVDDNVEIGSGTKIWHFCHILTNARIGENCI
                     IGQNVCIERDVIVGNRCKIQNNVSIYTGVTLEDEVFCGPSCVFTNVYNPRAFIERK"
BASE COUNT         1345 a         1036 c          943 g         1270 t
ORIGIN      
        1 tgagttaata caaaaacagc ttatcgaagc aaaaagagaa agaattgttc gtcgatcaaa
       61 agaagttgag accaatctga gaaaaggtaa agtcaaaagg gggactatgg aggatctctt
      121 aaaggacctc gagaatgatt aacatagtat gggatcctgg tttcaagaaa gcctatcaga
      181 aaaagatcaa aacggatgac aatctcaaaa aaccccttta gtaagcaact gagaactcat
      241 aaattaactg gtactctgaa aggcctgtgg gcgttcagtg tggattatga tactcgggtt
      301 attttctctt ttcttacaga tgacgaaata ttgctcatcg atattggcag ccatgacgaa
      361 gtgtattgac tgatctgatt cataacaaac cattaacaaa atttcacttg ttccttgtct
      421 tataaaccga agaaacctga tcggccagat aaacgcgttt agtatatgtt ctcgccagtt
      481 tgctcgaaaa acacctatta ttggaacgta taaatatcaa tatgttagcg tgttcggacc
      541 ctgatgattc accctttttt tcgccgaacc acgaccgcca aatgttctcc ctttcccctt
      601 gtgcctttta tcttgcacca tgcctctccg tatatttggc ttttacattt catttcgcaa
      661 ttcccgattc ccgttgcgcc atgcctcttg ctccttctac ctttcccttt caccttgatg
      721 cgtttcttta tatttttccg cccaacatag cgtcacgaat gccgaatgtt ttgcagccat
      781 cccgcagtta tcagcgttca tcctgtgaaa aagaagcttt acaacaatta aaaaaaagca
      841 gtttgtaaca ttgatggacg cagatggaca cagatgtaat gacaaaagct aatgatagac
      901 tgaattgttt cctccattgt gatagatcgg caatgaagga aacgctcagc ccatacgggc
      961 gggccaggca caagttttcc ccgaagggat gactattgac cggctggagt gctctatcaa
     1021 acaggcggtc aaaagatatc tcatcaagat ttatcagcgt ttatccgtgt tcatcctgta
     1081 ataaagatgc ttttaaacaa atgaacaaat cagtttgtaa caatgataca cgcagatgga
     1141 cacagatgta ataacaaaaa tggatgctca attatattgt ttcctccatt atgatagatc
     1201 ggcaatgaag taaacgctca gcccatatgg gcagatcaag cacaagattt cgcccgaagt
     1261 gctggatgtt gaccggctgg tatgctctat cagccagacg gtcaaagaat gctttatcag
     1321 ggtttatcgg cgttcatcag cgttcatcct gtaaaaaaga tgctttacaa caatatgcac
     1381 gcaaataaat acaaagatta tcataataaa attgactttt taacacagat tttataatta
     1441 cttatattga aaaaccgtag ggtaagcgtc cgcaacactg ctaaaatggc agcatcaaga
     1501 caattccatt catttatatg cctttcctgc ggtattctga gggatcggag gggttcccac
     1561 agcggtagcg tatcaaaata acgttctcta ccatagccac tgcctctgtt aatcttgttt
     1621 ttcgccttta atcttttgcc ttacgctttt tgcctttgat ctttcgcctc taacctttta
     1681 cctttaacct tgaaccttgt ctcttgcacc tcttaccttt caccatgcct ctccgtatat
     1741 ttggctttta cattccattt cgcaattccc gattcccgtt gcgccatgcc tcttgctctt
     1801 ttcacttttg accttgattc tttatccaca gaaagttcaa aatatggtaa aattgttttt
     1861 ttggttagca aataggaggt gttcatgaac tggagggaaa ggataactgc aaatcccctt
     1921 gtgtgccacg gcaaagcctg catcaaaggg actcgtatca tggtctccat cattctcgac
     1981 aaccttgctg ataatgccag cgaagaggat atactgaaaa gctatccgtc gctttctcca
     2041 gaagacatca aggcggccat agcatatgct gcagaattat cccgcgaaag gcttgtagcg
     2101 gtatcactct gacataagca tgttttttaa aatcgacgag aatcttcccg ctgatctagc
     2161 agaattgatg gtaacgcacg gatatgatgt caaaactgtc tatgatcaac agttgcaggg
     2221 cgttgaagat ctaatcctca tgggcagatg cgatgaagaa aatcgcatat tgattacctt
     2281 agataccgat ttttcagata tcacattata tcctccagaa aaacacgcgg gagtgattgt
     2341 tttgcgactt ggaaatcagg ccaaaaaaaa tatcctcgat gtttttcgca gaattattcc
     2401 agcccttgag cgagaaccta taaagcatca tctatggata gtagaagaaa cagtgatacg
     2461 aattcgtgga aaaaaagaat agccccttct cttttcccat aactcatcaa ccttgacctt
     2521 tcacaaacct atgttgtgct aactaattga tggaaagaaa agatctggaa aaccgcacga
     2581 aagcctttgc tgtacaaatc ataacttttg taacaaatct cccaaaaaat aaggtcaccg
     2641 atgtcctggg ttatcaacta atcaaatcag gcacatccat tggtgcaaat taccgtgaag
     2701 cacgatccgc tgaatcacat gatgatttca tacataagat tgggatagtg gaaaaagaag
     2761 cgagtgaaac ccaatactgg ttagaactcc tgcgagagtc cagtataggc aacctggatg
     2821 aacttcagtc attgcttcag gagtgttcag aattgtgtgc catattcaca gccacaggaa
     2881 agacggccaa atcaaaccgg aataaaaagc aataaaagca tcgcgaatct ccaaacgcgc
     2941 atttcgaatt gaactttaat aaaataataa gcctagactt ttgcatttgc ttgcacattt
     3001 cgcgttgcgc cattcgcctt gcccttctcc ctttcaccat gcctcaccgt atattttgct
     3061 tttgcattcc atttcgcaat tcccgattcc cgttgcgcca tgcctctcgc tccttttgcc
     3121 tacacagttt ctttaaattt ttcgccgaac aacgcatgct gaacgtttct attcttgttt
     3181 tgttacgcaa cagattaact gcgtattgtt ctcatcctaa caaccgaaag gaatctaacc
     3241 atgtataact ttgctctcct tggagctgcc ggctacatag caccgcgcca catgaaagct
     3301 attaaagata cgggtaacaa tctcgttgct gccatggatc cccatgatgc tgtcggtata
     3361 atggacagtt attttcctgg tgcacgtttt tttacagaat ttgagcgcct cgataggcac
     3421 gccgagaaac tgcggcgccg acatgatgat gaacaacgga ttcattgtgt gagcatcacc
     3481 tcgcctaact atctccacga cgcgcacatt cggtttgccc tccgggtagg cgccaacgcg
     3541 atctgtgaga aacccctggt gctcaacccc tggaacgtag accctctcca agagctcgag
     3601 cgagaatcag ggcggaaaat caacacgatt cttcagctac gccatcaccc cgccatcatt
     3661 gtattcaagg aaaaaatgca gggtatgcca cgagacaaaa aggtcgacat agatctcact
     3721 tacattacat cgcgcggccg gtggtatctg gtctcctgga aaggtgatat cactaaatca
     3781 ggaggggtgg caaccaatat cggtattcac ttcttcgaca tgcttcaatg ggttttcgga
     3841 tctgtccgtg aaaatacggt ccatgtttcg gaggccacca aagcaggggg ctatcttgag
     3901 ctggagcgag cccggatacg ttggttcctg tccatcgacc gaaccgatct cccacaggag
     3961 gtactggaag cgaataagac tacctaccgt tgcataactg ctgacaatga agaggttgaa
     4021 ttttccgatg gttttacgga cttgcatacg gtaagttatc agaagatcct tgcgggagaa
     4081 ggttttggac tcgttgatgt gaaaccttcc attgaaatcg cacacgatat acgaaatgcc
     4141 acacctatcg gtctcaaggg cgattaccat ccggcattga aaacatcaaa gagtgaaggg
     4201 taaagaatga gtcctaacga gagtcaaaaa cctatgggca ataggcaaga actggtaaag
     4261 aggtaaagta ggtaggtgga gataggaaca gctgatgaga aataacaaca actacttcgt
     4321 acacaaaagc tcatatgtcg atgacaacgt ggagattggt tcgggcacga aaatctggca
     4381 tttttgtcat attctcacca atgccaggat cggtgaaaac tgtattattg gccaaaatgt
     4441 ctgtatagag cgggatgtca ttgtcgggaa taggtgcaag atccaaaaca atgtctctat
     4501 ctataccggt gttaccctgg aggacgaggt tttttgcggt ccgtcatgtg tgtttacaaa
     4561 tgtttataat ccgcgagctt ttatagagcg caaa
//
LOCUS       DAOOMC010000171         4514 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdus sp. isolate AMR_MDS_2685
            NODE_23168_length_4514_cov_10.280556, whole genome shotgun
            sequence.
ACCESSION   DAOOMC010000171 DAOOMC010000000
VERSION     DAOOMC010000171.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864760
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdus sp. (wastewater metagenome)
  ORGANISM  Syntrophorhabdus sp.
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae; Syntrophorhabdus.
REFERENCE   1  (bases 1 to 4514)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4514)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,729
            Genes (coding)                    :: 2,708
            CDSs (with protein)               :: 2,708
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 7 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4514
                     /organism="Syntrophorhabdus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23168_length_4514_cov_10.280556"
                     /isolate="AMR_MDS_2685"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2699756"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(16..912)
                     /locus_tag="PK125_12755"
     CDS             complement(16..912)
                     /locus_tag="PK125_12755"
                     /inference="COORDINATES: protein motif:HMM:NF020419.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HPB39014.1"
                     /translation="MTDLGASVLARLKRKSKESGKSLQLLLQLFCQEEFLRRLSGSEY
                     ANHLVLKGGMFIYTLSNFSSRSTADIDFLLQKQPGGIEDIQRMVNEIISVDTGNNYIQ
                     LIPQGYKIISLQRKYKGVSFQLLGLVKNTKTPFNVDIGIGDVIVPEPEKLIIPVQLED
                     FIPARILTYSLESTIAEKFEALIQRQQLTSRMKDIYDIYYLSNQFDFDGNLLQQAILQ
                     TITNRKTFYEPDSLDKVIALSDDPDIQIRWRQFLYRTQLPELNLKLVFLGIDQFLRPV
                     WISIIQSKTQNKTWRARTSNWI"
     gene            complement(974..1579)
                     /locus_tag="PK125_12760"
     CDS             complement(974..1579)
                     /locus_tag="PK125_12760"
                     /inference="COORDINATES: protein motif:HMM:NF024734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin domain-containing protein"
                     /protein_id="HPB39015.1"
                     /translation="MKQKLEEIKTLAKRANGIFRTADLKKLGFTAYEIRKMLEKHFIE
                     RIKHGYYSLSETVENCSDAEIIARLFPEGVLCMYTALFYHGYSDRTPLAWDIAINKDV
                     SKSRFKIDYPYVQPYYMEAHLLLFGITNATYGDTDLKIFDKDRLICECVFFENKIDRE
                     TYNKAIQGYIADPNKNISNLLDYSKKRRILKKIKSRIGIWL"
     gene            complement(1753..3522)
                     /locus_tag="PK125_12765"
     CDS             complement(1753..3522)
                     /locus_tag="PK125_12765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002604483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HPB39016.1"
                     /translation="MIARIPKSQLLNLAYLLPFYVGNKLAQIFRLLQGGDIIDRFIAI
                     FSNLRFIGQHPLPSFNLQDLIIGLIFAVGIKLILVVKAQNRKKFRKGTEYGSARWGTA
                     KDIAPYVDKNPRNNIILTKTESLTMNSRPKNPKFARNKNVLVIGGSGSGKTRFFVKPN
                     LMQMHSSYVVTDPKGTILTECGTMLKRNGYHIRVLNTINFKRSMHYNPFGYIHSEKDI
                     LKLVNAIIINTKGEGEKAGEDFWIKAERLYYSALIGYIWYEAPEREQNFTTLLEMIDA
                     SEAREDDENFKNPVDELFEELEEKAPEHFAVRQYKKYKLAAGKTAKSILISCGARLAP
                     FDIQELRELMSYDEMDLDTLGDEKSALFVIISDTDDTFNFVVAILYTQLFNLLCDKAD
                     DVYGGRLPIHVRCLLDEFPNIGQIPRFERLIATIRSREISACIVLQAQSQLKALYKEH
                     AETIVGNCDSMLFLGGKEKTTLKEISEILGKETIDLDNTSENRGREKSHGISHQKMGK
                     DLMSQDELAVMDNSKCILQLRGERPFLSEKYDITRHPNYRFLSDADSKLAFIFRKEVD
                     KNMKLETDRVSEIYNMGFVSSDA"
     gene            complement(3519..4019)
                     /locus_tag="PK125_12770"
     CDS             complement(3519..4019)
                     /locus_tag="PK125_12770"
                     /inference="COORDINATES: protein motif:HMM:NF024099.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PcfB family protein"
                     /protein_id="HPB39017.1"
                     /translation="MQEEISQRSVTLIVQTGKMTGKVFLQAIQKYLDILKQQRELKAR
                     EKQLHPAYQGRMTIRQLMKERSGLSNIEIHDEHIHDFERIARRYGIEYAIKKERNREA
                     PHYLIFFRSRDTDVLQTAFNEFVKKRLKIQERPSLREKLQKLKLQNKERAAQVPEKVQ
                     RKEFVR"
     gene            complement(4019..>4514)
                     /locus_tag="PK125_12775"
     CDS             complement(4019..>4514)
                     /locus_tag="PK125_12775"
                     /inference="COORDINATES: protein motif:HMM:NF040303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF6017 domain-containing protein"
                     /protein_id="HPB39018.1"
                     /translation="KKELIKTDNIKTESIKTDKKDLIYQSIYPSRQKVPEKTNAEMDR
                     WMEFREIFKKNIEYEYILDRDPEMIPEILEIMIETACSKAKSFSINGTQISAERVRER
                     LLSLNSMHIEYVLDSLNQNTSKIQNVRAYLLATLYNAPKTINTFYRTMVNHDMYGNAR
                     NRRY"
BASE COUNT         1180 a          972 c          925 g         1437 t
ORIGIN      
        1 acttttagta gaaactcaaa tccaattgct tgtccgagct ctccaggtct tattctgagt
       61 tttactttga atgattgaaa tccaaaccgg tcgcaggaat tggtcaatcc ccaaaaatac
      121 aagtttgaga ttaagttctg gcaattgggt tcgataaaga aattgacgcc aacgaatctg
      181 aatatctgga tcatcagaca aggcaatgac tttatccaga ctgtcaggtt cgtaaaaagt
      241 ctttcgattg gtgattgttt gcaagatagc ctgttgaagg agattcccat caaagtcaaa
      301 ttggttcgag agatagtaaa tatcgtaaat gtccttcatg cgactcgtta attgttgcct
      361 ttggatcaat gcttcaaatt tttctgcaat tgtgctttct aatgagtacg ttagaatcct
      421 ggcaggtata aaatcttcca gttgcacggg gataattaat ttctcgggct ctggaacaat
      481 gacatcacca attccaatat cgacattgaa aggcgttttc gtgttcttta caagcccgag
      541 gagttgaaaa cttaccccct tatacttccg ttgtagcgat atgatcttat agccttgagg
      601 aatcagttgt atgtaattgt ttccagtatc gactgatatg atttcgttca ccattctttg
      661 aatgtcttca atgccgccag gctgcttctg taaaagaaag tcaatatccg cagttgaccg
      721 gcttgaaaaa ttactgagag tgtagatgaa catgccgcct ttaagtacca gatgattcgc
      781 atattctgat ccgcttaatc gacgcaaaaa ctcttcctgg caaaacagct gcaaaaggag
      841 ttgaagagat tttccgctct cttttgattt tctttttaga cgcgcaagaa ctgacgctcc
      901 caaatctgtc atcaatcaca acctttctta tagtgatctt attcgttttc caaagaacgt
      961 tcgaatcatt gatctacaac cagataccaa tccggctttt tattttcttt agtattcgtc
     1021 gcttcttaga gtaatcgagc aaattcgaaa tattcttatt cggatcagca atataaccct
     1081 gaattgcttt attgtacgtt tcgcgatcaa tcttattttc aaaaaaaaca cattcgcaaa
     1141 tcagtcgatc cttgtcaaat atcttcaggt ctgtgtcacc ataagtggca ttcgtgattc
     1201 cgaacaaaag caggtgtgct tccatgtagt aaggttgtac gtaagggtaa tcgattttga
     1261 aacgcgattt tgaaacatct ttattgattg cgatatccca ggccagcggt gtacgatcag
     1321 aataaccatg ataaaagagt gctgtataca tacagagcac cccttcaggg aatagtctcg
     1381 cgattatttc tgcatccgag cagttctcaa cggtctctga aagactgtaa tatccatgct
     1441 ttattctttc aataaagtgc ttttcaagca tttttcgaat ctcatacgca gtaaatccca
     1501 gtttctttag atcagcggta cgaaaaatac cattagcccg ttttgcaagt gtcttgattt
     1561 cttcaagttt ttgtttcata tttacctgtc tgccgctcac acacgttacg agcggcaata
     1621 tgatagcttc ttgaataata cttctcattt gcggggttgt caacaacttg ccgcttatat
     1681 cacgttaaag cggcaagttg gtaaatactg gaatttattg ttatttaaac atccaaaaat
     1741 atagatataa gtttatgcgt ctgatgaaac aaaacccatg ttgtaaattt ctgagacacg
     1801 gtctgtttct aacttcatgt ttttatcaac ttcctttcta aaaataaatg ccagcttaga
     1861 atctgcatct gaaagaaatc tgtaattcgg atgcctggtg atatcgtact tctctgacaa
     1921 gaacgggcgt tcgccacgaa gctgaaggat acattttgaa ttatccatca cagccagctc
     1981 atcctggctc atcaggtctt ttcccatctt ttgatgactt atcccatgag atttttcccg
     2041 tcctctgttt tcagaagtat tgtcgagatc aatcgtctcc ttacccagta tctccgagat
     2101 ttccttaaga gttgtctttt ccttaccacc caaaaaaagc atgctgtcac agttgccaac
     2161 aatcgtttca gcatgttctt tgtaaagtgc cttgagctga ctttgagcct gaaggacgat
     2221 gcacgctgag atctcacgac tgcgaatggt ggcaatcaag cgttcaaaac gtggtatctg
     2281 tcctatgtta ggaaattcgt ccagcagaca gcgcacatga attggcaacc gtccaccata
     2341 aacatcgtcc gctttatcac aaagcaagtt gaataactga gtgtaaagaa tcgcaaccac
     2401 aaaattgaag gtatcatcgg tatcactgat gatcacaaac aaggctgact tctcatcacc
     2461 aagagtgtcc aggtccattt catcgtagct catcagttcc cgcagttcct gaatatcaaa
     2521 cggagccagc ctggcaccac aggagataag gatggacttg gcggtcttcc cggcagccag
     2581 tttgtatttc ttgtactgcc ttacggcaaa gtgctccgga gctttttctt ccagttcctc
     2641 aaacaactca tcgaccgggt ttttaaagtt ttcatcatct tcccgtgctt cagaggcgtc
     2701 gatcatttca agcaaggttg tgaagttttg ctcccgttcc ggcgcttcat accagatgta
     2761 gccaatcagg gcagaatagt acaaacgctc cgcttttatc caaaagtctt cccctgcctt
     2821 ttctccttcc ccttttgtgt tgatgatgat ggcattgacc agtttgagaa tgtctttctc
     2881 agagtggatg tagccaaagg gattgtagtg catgctgcgt ttgaaattga tcgtattcag
     2941 tacccggatg tgatagccgt tgcgtttgag catcgtgcca cattcggtga ggatggttcc
     3001 ttttggatcg gtcaccacat agctggaatg catttgcatc agattgggct tgacgaagaa
     3061 acgggttttg cctgaaccag aaccaccaat aaccaacaca ttcttgtttc tggcaaactt
     3121 tgggtttttg gggcgactgt tcatggtgag cgattcggtt ttggtaagga tgatgttgtt
     3181 tcgtggattc ttgtcaacat acggagcgat gtctttcgct gttccccagc gggcagagcc
     3241 atattccgtg cctttacgaa acttctttct gttctgtgct ttcacaacca aaatgagctt
     3301 gatcccaaca gcaaatatca gaccgatgat gaggtcctga agattgaacg atggtaacgg
     3361 atgttgaccg ataaacctta ggttcgagaa gatagcgatg aaccggtcaa tgatatctcc
     3421 accctgaagg agccgaaaga tctgggcaag cttgttgcca acatagaagg gcaggagata
     3481 tgctaaattc aataactgac ttttggggat tcgtgctatc accggacaaa ctcctttctc
     3541 tgaacttttt caggcacctg cgcggctctt tccttgttct gaagcttgag tttctgaagc
     3601 ttttcccgaa gtgatggacg ctcctgtatc ttgaggcgtt tcttcacgaa ctcgttgaag
     3661 gcagtttgca gcacatccgt atcccgggac cgaaagaaaa ttaaataatg aggtgcctct
     3721 ctgttacgtt ctttcttgat cgcatattca atgccatatc ttctggcaat tctttcaaaa
     3781 tcgtgaatat gttcatcgtg aatttcgata ttcgaaaggc ctgatctttc tttcatcagt
     3841 tgcctgatgg tcatgcgacc ctggtaagca gggtggagct gtttttctct cgcttttaat
     3901 tcacgctgtt gtttgaggat atcgagatat ttttgaatcg cctggagaaa gacctttccg
     3961 gtcattttcc cggtttgaac aatcagagta acgcttcgtt gtgaaatttc ctcctgcatc
     4021 agtaccgcct gttcctggca ttgccataca tgtcatgatt taccatggtt ctgtagaaag
     4081 tatttattgt tttaggagcg ttatagagcg tggcaagcag ataggctctg acattctgga
     4141 ttttactggt gttttgattt agcgagtcca ggacatattc gatgtgcatt gagttgaggc
     4201 tcaggagcct ctctctgacc cgctcagcag agatttgagt gccattgatg ctaaatgatt
     4261 tggcttttga acaggcagtt tcgatcataa tctccaggat ttccggaatc atctccggat
     4321 cgcgatcaag gatgtattcg tattcaatgt ttttcttaaa aatttccctg aattccatcc
     4381 atcgatccat ctcagcgttt gtcttttcag gaactttttg tcgtgatgga tagatagatt
     4441 gatagattaa gtctttttta tcagtcttaa tagactcagt cttaatatta tcagtcttaa
     4501 ttaactcctt tttt
//
LOCUS       DAOOMC010000172         4489 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdus sp. isolate AMR_MDS_2685
            NODE_23323_length_4489_cov_6.571944, whole genome shotgun sequence.
ACCESSION   DAOOMC010000172 DAOOMC010000000
VERSION     DAOOMC010000172.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864760
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdus sp. (wastewater metagenome)
  ORGANISM  Syntrophorhabdus sp.
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae; Syntrophorhabdus.
REFERENCE   1  (bases 1 to 4489)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4489)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,729
            Genes (coding)                    :: 2,708
            CDSs (with protein)               :: 2,708
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 7 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4489
                     /organism="Syntrophorhabdus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23323_length_4489_cov_6.571944"
                     /isolate="AMR_MDS_2685"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2699756"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..364
                     /locus_tag="PK125_12780"
     CDS             <1..364
                     /locus_tag="PK125_12780"
                     /inference="COORDINATES: protein motif:HMM:NF012609.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PEP-utilizing enzyme"
                     /protein_id="HPB39019.1"
                     /translation="CKEGTRSSPYDTRYDTLPTCGSPGVAEGIARTVFKEEDLAAIQE
                     GDILISVSTSPAWTPIFGMIRGVVVDRGGSLSHAAIVGREYGIPVVMNTFEGTAKIKS
                     GQRIRIDANLGTVFILDK"
     gene            450..1106
                     /locus_tag="PK125_12785"
     CDS             450..1106
                     /locus_tag="PK125_12785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB39020.1"
                     /translation="MFLFVQQWEIIRGKGAEYTDFVLRRHLPVMTKVGLNMIGGFHVI
                     VGSGPRISAVSLAHDFLNLQKAIESEEFIEVTRELHEFVYNYNSAVLKRTGRVNMEGY
                     GIEVGTWRFNQYYKLIYNTEKEYTRFLQNDYIPTLTKLGIRIKAEWQVILGAGLRILL
                     EGVTTNLSDVAQAIMSDEFRSLRRQLLSNFAGNYSSRILVPTGRVEIAYLLGEMTKAL
                     "
     gene            1171..2499
                     /locus_tag="PK125_12790"
     CDS             1171..2499
                     /locus_tag="PK125_12790"
                     /inference="COORDINATES: protein motif:HMM:NF025888.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB39021.1"
                     /translation="MAKNGSIYWNPIMETMPREKLRELQVKKFKRVFEWAYKNSPFYK
                     KLYKDAGMEPGDIKTLEDIKKVPKTDKGMLREVQNRPPFPYGDMLAVPLQDVTEFRQT
                     SGTTGTPVYQADTWQDWEWWAESWAYILYAQGYRDTDRMFLPFGYNIFVAFWACHYAA
                     EKLGCEVVPGGVLDTEARIMKMQELKCTAFGATPTYVLGMADTARKIGIDPRSIGIQR
                     ITCAGEPGASIPTTKQRIEEAWGAKVYDHIGATEIGAWSYMCTAQKGLHINEAFFLVE
                     IEDTETGEIIEEPMKNGKMIITAFDRQAKPCIRFDSKDVIRWADYECECGRTFRIIDG
                     GVVGRADDITKVKGVLLAPTAIEEVVRSFNELSDEYEVIVTKKGDIDDIMLKIEIKPG
                     FEKQEADILNRLKDQLRVKTNLGYKIEIHPYESLPRYALKAKRFKDLRPH"
     gene            2517..2696
                     /locus_tag="PK125_12795"
     CDS             2517..2696
                     /locus_tag="PK125_12795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB39022.1"
                     /translation="MAKEYKIDEMDAKIQEIKKAVEEIEKLGGDIEAVKKNLVRLRAS
                     TKMLELNISDVKLVM"
     gene            2788..3813
                     /locus_tag="PK125_12800"
     CDS             2788..3813
                     /locus_tag="PK125_12800"
                     /inference="COORDINATES: protein motif:HMM:NF013491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HPB39023.1"
                     /translation="MEKWIFWFDDIGAEANDFVGKKCANLGEMTKLGMRVPYGFAISV
                     NGFEKFMNLTGLSQKVKECVESERDRLQQIEVCQNLSRTIQGLVEATPMPDAMQSELA
                     AHYRELCAKAGREIIPVAVRSSGVISMPGQMDTYLNVIGEDAVVEHVRKVWASSYTTR
                     AITCRIEQDRPVEWAPIGVAVMMLVHAKSAGVVLTVLPTSGDTSKVVIEGNWGLGESV
                     VSGEITPDSFVVEKDDLRITSRNITHKKGMVQRGEHGTVYVPVPDELQDKPCLDDDEI
                     LEITRTALRVEQHFGVPQDMEWVVDRYLPFPENIFWVQARPARFTKANATEDIDYIID
                     LMAQIFK"
     gene            4058..4243
                     /locus_tag="PK125_12805"
     CDS             4058..4243
                     /locus_tag="PK125_12805"
                     /inference="COORDINATES: protein motif:HMM:NF013481.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016625 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, iron-sulfur
                     protein as acceptor [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase C-terminal
                     domain-containing protein"
                     /protein_id="HPB39024.1"
                     /translation="MFKEPLQNAGLAAGHVVAKMDTILDEYYAYMGYDANGVPTAAKL
                     KELGLTDAANEMEKFRK"
BASE COUNT         1352 a          826 c         1177 g         1134 t
ORIGIN      
        1 ttgtaaggaa ggtacccgat cttcccctta tgacacaaga tatgatacac tacctacttg
       61 tggttctcct ggagttgctg aaggcattgc aaggactgtg ttcaaggaag aagaccttgc
      121 agctatccaa gaaggggaca tcctcatatc ggttagcacc tctcctgcat ggacaccaat
      181 cttcgggatg atacgcggtg ttgttgtgga caggggtgga agcctttccc atgcagcaat
      241 tgtagggaga gaatacggca ttcctgttgt catgaacaca tttgaaggca cagcaaagat
      301 aaaatcagga caacggatta ggattgacgc gaatttagga acggtcttta tactggataa
      361 gtgaaaaata aggcaggagt cgagaacggt tagtggtgag gatcacaaat cggaaatcgg
      421 tactatctaa tacagagagg tgagtaatca tgtttttatt tgtccagcag tgggaaatca
      481 tcagagggaa aggtgccgaa tacaccgatt ttgtgttacg aagacacttg cctgtaatga
      541 cgaaagtcgg cctcaacatg attggcggat ttcatgttat tgtaggatca ggcccgagaa
      601 ttagtgcagt atccttggca catgactttt tgaacctcca gaaagcgatt gaaagtgaag
      661 aatttattga ggtaacaaga gagcttcatg agtttgtcta taattataat agtgctgtcc
      721 tcaagcgtac tggaagagtt aatatggagg gatatggcat tgaggtcggc acgtggcggt
      781 ttaatcaata ctacaagttg atttacaaca ctgagaaaga gtacacgcga tttcttcaaa
      841 acgattatat tcctacgctg acaaaactgg gtatacgaat taaggcagaa tggcaggtca
      901 tacttggtgc aggtctccgc atccttcttg aaggagttac cacaaacctt tctgatgttg
      961 ctcaggcaat catgtcagat gagtttaggt ctttacgacg gcaactgctt tcaaattttg
     1021 caggaaatta cagcagcaga atccttgtcc ctactggaag ggtcgagatt gcgtatcttt
     1081 taggggaaat gactaaagcc ctgtaaaggg aattgttgta cgcgagaagg tttgtaagac
     1141 ttcacgaaaa atttaaaaag gaggatgtcg atggccaaga atggaagcat ttactggaat
     1201 ccgatcatgg agacaatgcc ccgggagaaa ctccgtgagc ttcaggtgaa aaagttcaag
     1261 cgggtttttg aatgggcata taaaaactca ccattttaca agaagcttta taaggatgct
     1321 ggtatggaac cgggggatat aaagaccctt gaagatatca agaaggtgcc caagacagat
     1381 aagggcatgc tgagagaggt ccagaacagg cctccttttc cttatggtga tatgcttgca
     1441 gttcctttgc aggatgttac agaattcaga cagacaagcg gtactactgg aacaccggta
     1501 tatcaggccg atacctggca ggactgggag tggtgggcag aaagctgggc atacatcctt
     1561 tatgcacagg gttacagaga tacagatcgt atgtttctcc cttttggcta caatatcttt
     1621 gttgccttct gggcttgcca ttatgcagca gaaaaattag ggtgtgaggt tgtgccgggt
     1681 ggtgtccttg atacggaggc acgaataatg aagatgcagg aactcaagtg cacagccttt
     1741 ggagctactc ccacctatgt tcttggtatg gctgatactg ctcgaaaaat tggcattgac
     1801 ccaaggtcaa ttggtataca gagaataacc tgtgcaggtg agccgggcgc gagcattccc
     1861 acaacaaaac agcgcattga agaagcatgg ggtgcaaagg tttatgatca tatcggcgca
     1921 accgagatag gggcctggag ctatatgtgc accgcccaga aaggcctcca tatcaacgaa
     1981 gccttcttcc ttgtggagat tgaagataca gaaacgggcg agattatcga agaacccatg
     2041 aaaaacggta agatgattat cactgccttt gaccggcagg caaaaccatg tattaggttt
     2101 gactcgaaag atgttatccg ctgggcagat tatgaatgcg agtgtggtcg taccttcaga
     2161 atcattgatg gcggggttgt gggcagagcc gatgacatta ctaaggttaa gggtgtgctt
     2221 ttggccccaa cagcaattga ggaagtggta aggagcttta acgaattgag cgatgagtat
     2281 gaggtcatag tcacaaagaa gggtgacatt gatgatatta tgctaaagat tgagataaag
     2341 cctggttttg aaaagcagga agccgatatt ctgaaccgcc tgaaagatca gctcagagtt
     2401 aaaaccaacc ttggatacaa gatagagatt catccatatg agagtcttcc acggtacgcg
     2461 ttgaaagcaa agagattcaa agatctgaga ccacactaag caaggagggc acgatcatgg
     2521 caaaagaata taaaattgat gaaatggatg caaagatcca agaaataaaa aaggcagtcg
     2581 aagagataga aaagcttgga ggagatatcg aggcagtcaa aaaaaacctt gtgagactgc
     2641 gtgcaagcac taagatgctt gaattgaaca tttctgatgt aaaactggtc atgtaaaaga
     2701 tgtctttacc cccgcctctc ctgcaaaatt tatttttgta aaataaggtt tgcaggggag
     2761 gcaccggggg ccaggggtta cgcgagtatg gagaagtgga tattttggtt tgatgatatt
     2821 ggtgctgagg caaatgattt tgtcgggaaa aaatgtgcaa atcttggcga gatgaccaag
     2881 cttggcatga gggttcccta tggatttgca atctcggtaa atggttttga gaaatttatg
     2941 aacctcaccg gcctttccca gaaggtaaaa gaatgtgttg agagtgaaag ggatagactc
     3001 cagcagatag aggtttgcca aaatttaagc cgtacaatcc agggtcttgt cgaagcaacg
     3061 ccaatgcctg atgctatgca aagcgaactt gctgctcatt acagagagct ctgcgcaaag
     3121 gcaggcaggg aaatcattcc tgttgcagtt cgttcaagtg gcgttattag catgcctggt
     3181 cagatggata catacctgaa cgtaataggt gaagatgctg ttgttgaaca cgtgagaaaa
     3241 gtatgggcaa gttcgtacac gacacgggcc ataacatgca gaattgaaca ggatagacca
     3301 gttgagtggg cacctattgg cgttgctgtg atgatgcttg ttcatgcgaa gtcagcaggg
     3361 gttgttctca cggtcttgcc aacatccggc gatacatcga aggttgtgat cgagggaaac
     3421 tgggggttgg gagaaagcgt tgtaagtggt gagattaccc ccgattcgtt tgttgtagaa
     3481 aaggatgatt taaggattac aagtcgcaac attacccaca aaaaaggaat ggtacagcgc
     3541 ggagaacatg gtaccgtata tgttccggtg ccagatgaat tgcaggataa accatgtctt
     3601 gatgatgatg aaatccttga gatcacgcga acggcacttc gtgtggagca gcacttcggt
     3661 gttccacaag atatggaatg ggttgtggat aggtatctcc cattccctga aaatatcttc
     3721 tgggtgcagg ccagacctgc cagattcaca aaggcaaacg ctactgagga tatcgactat
     3781 atcattgatc ttatggcaca aattttcaaa taattttgtt gtaagaaggg cagaagaaaa
     3841 gggatgtcag tatatcgaaa cagactactc agactcagtt tacccaaagg agaggcagca
     3901 gagataccaa ctgagagcaa cgttatcaga gattttctcg gcggcaaaga cttcggcatc
     3961 aactatttgt acaaaaacct ttgccgggta ctgatccgtt cagcccggcc aacatcctcc
     4021 tgtttgttcc tggaatttta ggagtcacca agagcggatg tttaaagagc ccttacagaa
     4081 tgcaggactg gccgccggtc atgtagtagc caagatggac accattcttg atgaatacta
     4141 cgcttacatg gggtacgacg caaacggtgt tccgactgca gcaaagctga aagagttggg
     4201 attgaccgat gcggcaaatg aaatggagaa gttccgaaag taggaactgt tcatgtatga
     4261 taatagggga ttcgaccccc cccttttttt tgattcgcat ttaaggaagg ctgtgtattg
     4321 tcgtccgcat atagtatatt gataatcctc gataaaatgt aattctacaa ttctttgaag
     4381 ttggttggtt gtatactgtg ttcgttagcc aaaaaaacag caaggaaaaa gtataggtag
     4441 tgtaaaatct tttgtgtaaa ttgaagagga tgacaaaaag gatgtacct
//
LOCUS       DAOOMC010000173         4424 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdus sp. isolate AMR_MDS_2685
            NODE_23770_length_4424_cov_6.346532, whole genome shotgun sequence.
ACCESSION   DAOOMC010000173 DAOOMC010000000
VERSION     DAOOMC010000173.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864760
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdus sp. (wastewater metagenome)
  ORGANISM  Syntrophorhabdus sp.
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae; Syntrophorhabdus.
REFERENCE   1  (bases 1 to 4424)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4424)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,729
            Genes (coding)                    :: 2,708
            CDSs (with protein)               :: 2,708
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 7 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4424
                     /organism="Syntrophorhabdus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23770_length_4424_cov_6.346532"
                     /isolate="AMR_MDS_2685"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2699756"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1386)
                     /gene="groL"
                     /locus_tag="PK125_12810"
     CDS             complement(<1..1386)
                     /gene="groL"
                     /locus_tag="PK125_12810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004585059.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HPB39025.1"
                     /translation="MAKEIKYDQGVRESLLKGVNTLADAVRLTLGPRGRNVILEKSFG
                     SPTVTKDGVTVAKEIEIEDKFENMGAQMVKEVASKTSDVAGDGTTTATVLAQSIYREG
                     AKLVAAGHNPMELKRGIERAVEVVVDELRKLSKPTKDQKEITQVGTISANNDETIGNI
                     IAEAMGKVGKEGVITVEEAKSMETSLEIVEGMQFDKGYISPYFVTNPEKMEAVMEEPL
                     ILINEKKISSMKDLLPVLEQVAKMGKPLLIIAEDVEGEALATLVVNKLRGTLKVAAVK
                     APGFGDRRKAMLEDIAILTGGQMISEELGIKLETVGLKDLGQAKRIVIDKDNTTIVDG
                     AGEKKDIEGRVKQIRAQIDETTSDYDREKLQERLAKLVGGVAVINVGAATEAEMKEKK
                     ARVEDALNATKAAVEEGIIPGGGVAFIRAIPELDKLNIEGERQFGVKIVKKALEEPIR
                     WIANNAGHDGSI"
     gene            complement(1403..1693)
                     /gene="groES"
                     /locus_tag="PK125_12815"
     CDS             complement(1403..1693)
                     /gene="groES"
                     /locus_tag="PK125_12815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003966899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HPB39026.1"
                     /translation="MKIKPLQDRIIVKRLEEEEKTKGGIIIPDAAKEKPQEGKVIAVG
                     DGKILENGQKLALTVKVGDKILFGKYSGTEIKIDGEEHLILREDDVLGIVED"
     gene            complement(1795..2574)
                     /locus_tag="PK125_12820"
     CDS             complement(1795..2574)
                     /locus_tag="PK125_12820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB39027.1"
                     /translation="MLPRRHILDVWEFIKDEKDLLSMSTITLAIDAIKYLDMESKKDH
                     LVEALELNEFICFMHPTKRPKNRSLLFHVVSDLLGLLMFGVPRKRKHAIDSIEIVNYS
                     EESMEIYPVIEVWNDLKNKVYTKKHGATSIIHGFERKIRVEMDIIERYPFVDDILFES
                     QESIKEWLPVLTSYYDKKSKEIKDIYKKWWSLWGCKKKAHEALSGMIERLSSQALREY
                     NITINQDSLMKTILQEKDRNKNENEEFSRWYKEGVNLLLKI"
     gene            complement(2574..3491)
                     /locus_tag="PK125_12825"
     CDS             complement(2574..3491)
                     /locus_tag="PK125_12825"
                     /inference="COORDINATES: protein motif:HMM:NF013705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ C-terminal domain-containing protein"
                     /protein_id="HPB39028.1"
                     /translation="MAVKKDYYEILGVAKGASEDDIKKAYRRLAKKYHPDKNPGNKEA
                     EEQFKLISEAYAVLSDKAKRKEYDAYGMGGFQQRYSEEDIFKGANFGDLFRDLGFGGG
                     DIFSMIFGRQAGRTGRQRSQAYDFGDYITREQQPPKNVDLNYELEIPFMDAVRGTEKR
                     ISFATQQGTEEVNIKIPRGISTGKKLRLQGKGNRDPRTGRVGDLYITVKVGDHPVFKR
                     AGNDIHITKEIGLTDALLGTTVEVSTIDGPKVVKIPAGTKKVRLRGLGIPDMKGNRGD
                     QYVETEVAIPKKLTDKQKTLIEELRREGL"
     gene            complement(3493..4317)
                     /locus_tag="PK125_12830"
     CDS             complement(3493..4317)
                     /locus_tag="PK125_12830"
                     /inference="COORDINATES: protein motif:HMM:NF014046.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ARMT1-like domain-containing protein"
                     /protein_id="HPB39029.1"
                     /translation="MKLMDHCYGCLKDLIEKAVALSQGDEEIAFQAYSMVDNLWNTGS
                     TPPDIANKLHRFIKSKTGVTDPYFSIKTKEVEAAQKAICELRPAFPETLEGFIKLSAL
                     GNSTDFFCHHEYKIEGFDFSGDIDKITEEIYTRSNVVLMLSDNAGEFLFDVGLIRFLE
                     DTGKTVYYAVKENPVQNDLSIPDVERFGFRKMFPNIISTETDEVGIRREDLRGTVKEC
                     WENNAIVIAKGMGNYETISEFSDNRSVIHIMKVKCPTVASAVGGKVGQYIAIIGGG"
BASE COUNT         1047 a         1097 c          804 g         1476 t
ORIGIN      
        1 gatggatccg tcgtgccctg cgttgttagc aatccatctg atcggctctt caagggcttt
       61 cttgactatc ttgacaccaa actgcctctc cccttcaatg ttgagcttat caagttctgg
      121 aattgccctg atgaatgcaa caccgccgcc gggaataatt ccttcttcca cggctgcttt
      181 tgttgcattg agtgcatctt ccacgcgggc ttttttctct ttcatctcag cttctgttgc
      241 tgccccaaca ttgataacag cgacaccacc cacgagcttg gcaagtcttt cctgaagttt
      301 ttctctgtca taatcggatg ttgtttcatc aatctgggct ctgatctgct taaccctgcc
      361 ttcgatatcc ttcttttcac cggcaccgtc gacaatagtt gtattgtctt tgtcgatcac
      421 aatcctcttt gcctggccaa gatctttcag tccaacggtc tcaagtttaa taccgagttc
      481 ttctgagatc atctgtccgc cggtaaggat agcgatatct tccaacattg cttttcttct
      541 atcaccaaaa ccaggggctt taacagcagc cactttcaga gtacctctca gtttgttaac
      601 gactaaagtc gccagagcct caccctcaac atcttcagca ataatcaaaa gaggttttcc
      661 catttttgcc acctgctcaa gaacagggag aagatccttc atactgctga tctttttttc
      721 attaatgagg atcaaaggtt cctccatcac tgcttccatt ttctcagggt ttgtcacaaa
      781 atatggtgat atatatccct tgtcgaactg cataccctcg acgatttcaa gggatgtctc
      841 catgcttttt gcttcttcca cggtgataac accctctttg ccaactttcc ccattgcttc
      901 agcaatgata tttccaatcg tttcatcgtt atttgcagaa atggttccca cctgggtaat
      961 ttctttctga tcctttgtgg gttttgaaag ctttctgagc tcatcaacga caacttctac
     1021 tgctctttca atacctcttt taagttccat cgggttgtgg ccggcagcta caagttttgc
     1081 accttcgcga taaattgact gagcaagtac agtagccgtg gtggttccat cgccagctac
     1141 atcgcttgtc ttggatgcca cttcttttac catctgggca cccatgttct cgaatttatc
     1201 ctcaatttct atttctttcg caacggttac accgtctttt gtaaccgtgg gcgaaccaaa
     1261 agacttctcc aagattacat ttcttcctct aggtccgaga gtcaatctta ctgcatctgc
     1321 aagagtatta acacctttta gaagtgactc cctgacgccc tgatcatact taatttcttt
     1381 agccatcctt tagcctcctt tcttagtctt ctacaattcc gagaacatca tcttctctca
     1441 ggatgagatg ttcttcaccg tcaatcttga tttcagtgcc ggaatactta ccgaagagaa
     1501 tcttgtcgcc gaccttaact gttaaagcta gtttctggcc gttttcgaga atctttccgt
     1561 cacccacggc aatcactttg ccttcttgag gtttttcttt agctgcatcg ggaataatga
     1621 tgcctccctt tgtcttttct tcctcctcaa gccttttgac gatgattctg tcttgtaatg
     1681 gtttaatctt catcgtatca cactcctttc aaaaaaatta tatttggcaa ttggtaaacc
     1741 agaatgctaa aaatactata atgataagag gtagtttttg tcaatagggg caaattaaat
     1801 ttttaacaat aaatttaccc cttctttata ccatcttgaa aactcttcat tttcgttttt
     1861 gtttctatct ttttcctgca gtattgtttt catcagacta tcctggttga tggtgatgtt
     1921 atattccctc aaggcttggg aagaaagacg ttctatcata cctgaaagcg cttcatgcgc
     1981 ctttttctta caaccccaga ggctccacca tttcttgtaa atgtccttaa tctctttgct
     2041 ttttttatca tagtaactgg taaggacagg aagccattct ttgatgcttt cctgggactc
     2101 aaacagaata tcatcaacga agggataccg ttctatgatg tccatttcaa cccgtatctt
     2161 ccgttcaaaa ccatgtatga tgcttgtggc tccgtgtttt tttgtatata ctttattttt
     2221 gagatcattc cagacctcaa taactggata tatttccata ctctcttcgg aatagttcac
     2281 aatctcaatg ctgtcaatgg catgttttct ttttctcgga acaccaaaca tgagaaggcc
     2341 caatagatct gaaacgacat gaaaaaggag tgatctgttt ttaggtctct ttgtgggatg
     2401 catgaaacat ataaactcat tgagctcaag ggcttcgaca agatgatctt ttttggactc
     2461 catgtccaag tattttattg catcaatggc gagggtaatc gtggacatgg atagcaggtc
     2521 tttctcatcc ttgatgaact cccagacatc aaggatgtga cgccgtggaa gcattataat
     2581 ccctctcgcc tcagctcctc gatcagtgtt ttttgcttgt cagtcagttt tttaggtatc
     2641 gctacctctg tctcgacgta ctggtcgcct ctgttcccct tcatatcggg aatccccagg
     2701 cctcgtaacc tgactttttt ggtgcctgca ggaatcttga ccaccttcgg cccgtcaatg
     2761 gtagatactt ccacagtggt acctagtaat gcgtcggtaa gacctatctc cttcgttatg
     2821 tggatatcat tgccagctct tttgaagacg ggatgatcac caactttgac agtaatgtat
     2881 agatcgccaa ccctaccagt tctcggatct ctgtttcctt ttccttgaag cctcagcttc
     2941 ttgcctgttg atatccctcg gggtatttta atgttaacct cttcagtgcc ctgttgggtg
     3001 gcaaaagaga ttcttttctc tgtacctctg actgcatcca tgaacgggat ttcaagctca
     3061 tagttgaggt ccacgttttt tggtggctgc tgttctctcg ttatgtaatc cccgaaatcg
     3121 taggcctgag acctctgccg gccagttctg ccagcctgtc ttccaaagat catgctgaaa
     3181 atgtcgccac ctccgaatcc aaggtccctg aaaaggtctc caaagtttgc ccccttgaat
     3241 atatcttctt cagagtaacg ctgctggaag ccgcccatac catatgcgtc atattccttt
     3301 ctctttgctt tgtcacttag cactgcatag gcttcgctga tgagcttgaa ctgttcctct
     3361 gcttctttat tgccggggtt tttgtcagga tgatatttct ttgcaagcct cctgtatgct
     3421 ttttttatgt catcctctga tgcccctttg gctaccccaa gtatttcgta gtaatctttt
     3481 tttaccgcca ttttatccac cacctatgat agcaatatat tgcccgacct taccgcccac
     3541 agcgctggct actgtcgggc acttcacctt catgatatgt atgactgatc tattgtcact
     3601 aaattccgat atggtctcat aattccccat acccttggca attacaatgg cattattctc
     3661 ccaacattcc tttaccgttc ccctcaagtc ttctcttctt atgcccactt cgtcagtttc
     3721 tgttgagata atattcggga acattttcct gaagccgaat cgctcaacat cgggtattga
     3781 aaggtcgttt tgaacagggt tctcctttac agcataataa acagtttttc ctgtatcttc
     3841 taggaaccgt attaatccca catcgaacaa gaactcacca gcgttgtcag aaagcatcag
     3901 aacaacatta cttctagtat aaatctcctc tgtgatttta tctatatccc ctgaaaaatc
     3961 aaatccttca attttgtact catggtggca gaagaaatcc gttgagttac ccagagcaga
     4021 gagtttgata aagccttcta aagtttcagg aaaagctggg cgcaactcgc atatcgcctt
     4081 ttgtgctgct tcgacctcct ttgttttgat tgaaaaatac gggtcagtaa cacctgtttt
     4141 gcttttgatg aatctgtgga gcttgtttgc aatatcgggt ggagtgcttc ctgtattcca
     4201 gagattgtca accatcgaat atgcctgaaa agcgatctct tcatcaccct gagacaaagc
     4261 aacagctttc tcaatgaggt ctttcaggca cccataacag tgatccatta gtttcatgtt
     4321 gaacattata agaatataac gaaacttatt ccaattcaac ctgttgactt gaagaaaaac
     4381 caagaagatt gggcttgcaa gaattcacca ggaagggtac aata
//
LOCUS       DAOOMC010000174         4424 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdus sp. isolate AMR_MDS_2685
            NODE_23771_length_4424_cov_6.263676, whole genome shotgun sequence.
ACCESSION   DAOOMC010000174 DAOOMC010000000
VERSION     DAOOMC010000174.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864760
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdus sp. (wastewater metagenome)
  ORGANISM  Syntrophorhabdus sp.
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae; Syntrophorhabdus.
REFERENCE   1  (bases 1 to 4424)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4424)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,729
            Genes (coding)                    :: 2,708
            CDSs (with protein)               :: 2,708
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 7 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4424
                     /organism="Syntrophorhabdus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23771_length_4424_cov_6.263676"
                     /isolate="AMR_MDS_2685"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2699756"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(225..1568)
                     /locus_tag="PK125_12835"
     CDS             complement(225..1568)
                     /locus_tag="PK125_12835"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HPB39030.1"
                     /translation="MTMISLTPLEVWIRNKIEIGLSTPDRSFQDALNNYHLQRLKDVI
                     NYARQKSQFYARHLALVPEEPLNTLADMSQLPFTMPSDLSDNPWHFLTVHQEDITRIV
                     TLRTSGSTGKPKRIFFTQEDLELTIDFFAIGMSTFVKPGQRVLILLPGDQPDSVGELL
                     ARGLKRIKVDSFAHGPVHDVKTAIDKIVSLEIDSLVGIPTQVLALARSRDGAVLKKGM
                     IKNVLLSTDYVPQSIIKSLEEIWGCSVFNHYGMTEMGLGGGVECDAHNGYHLREADLY
                     FEVINPETGEIIDDGSSGEIVFTTLTRKGMPLIRYRTGDIARFIREPCPCGTVLRRME
                     RVRGRWDGRVFLGMGQTITLPEMDEALFSVPWLLNYRVTMTNHNERILLGIEVYADSD
                     HSHQGPEVTRVLMDIPQISKAIERKSLELGPVQFREDDWFTTGVAKRGIIVNERG"
     gene            complement(1865..2305)
                     /locus_tag="PK125_12840"
     CDS             complement(1865..2305)
                     /locus_tag="PK125_12840"
                     /inference="COORDINATES: protein motif:HMM:NF021252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-GCAxxG-C-C family protein"
                     /protein_id="HPB39031.1"
                     /translation="MDNIFLQIMKLYAKGFCCSQIIVMLAMEAEGKKNPDLVRSLGGL
                     CFGVNWSGEVCGALSGGACLISLYSGKGSEGETQDNRCTTMIGDLVEWFTLAAHEEYG
                     GTRCHEILEKYPDRSMCGLIVSETYKKCMDILADHGLQPACHKR"
     gene            complement(2428..2640)
                     /locus_tag="PK125_12845"
     CDS             complement(2428..2640)
                     /locus_tag="PK125_12845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB39032.1"
                     /translation="MTELNKTGSDKGWICHGCESPLEVQTVRLEYRGNIFAVHLPVCP
                     LCGMILIDEELATGEMAEGEQIVEDK"
     gene            complement(2637..2927)
                     /locus_tag="PK125_12850"
     CDS             complement(2637..2927)
                     /locus_tag="PK125_12850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB39033.1"
                     /translation="MTAEQKNAYQHISLFISEEVERLMNDRGILKEDVQRTIHHAETI
                     GEKFINPTTGRFLASFRPDRVTYWVEYSVVGEDYHVHTTYSHRMELKRKGKP"
     gene            complement(3092..4327)
                     /locus_tag="PK125_12855"
     CDS             complement(3092..4327)
                     /locus_tag="PK125_12855"
                     /inference="COORDINATES: protein motif:HMM:NF015418.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HPB39034.1"
                     /translation="MHNKNLTVSVEEALEIVLRSVNPLSFENIPLMRAFNRILYEDVV
                     SDRMMPPVADSAMDGYAIIADDTRGASLNCPVKLHVVGEIRAGSSSTGYQVSKGTAIR
                     IMTGAPIPQGADSVVRFEDTEEESGYIKLFCETEKYENYREAGEHIREGEHILSKGKR
                     LNSADVGILASLHRNTVKVYKRPTVSVISTGDELADTGEEIQIHQIRNSNAYTICSEV
                     TKYGGHARYLGIAKDSLGEMRKIFANALKSDVIISTGGVSAGKYDLVKEVCSDLNIEI
                     HFDRVNIKPGRPCVFGTKGDKMVFGLPGNPVPALTSFIQFVRPALLRLMGATKIRKPI
                     VNASLEQDITSGKIHHLVRGRYTIINNEFYVSKTSHQKPSMLRSMSDANCLIIIPETV
                     TKVMAGDRVAIQLIDHDEI"
BASE COUNT         1143 a         1074 c          841 g         1366 t
ORIGIN      
        1 agaaaccttg ccttcctatt gcatttcatc atttctttcc tgtgttatca attgtcgggg
       61 ctttgaaagc ttttgagatg tttgaaccag catttccaca ttttatacca cacgatcata
      121 tccaccttat tcatcgtttt ttggcatcca ggtgccccac tcgatttaaa tgctggattg
      181 tcttacatct tattttttct tataatattc tttccgcctc cactttaccc acgttcattc
      241 acaatgattc ctcgttttgc cactcctgtg gtgaaccaat catcttctct gaattgtaca
      301 ggccccaatt cgaggctctt tctttcaatg gctttggata tctgtggtat gtccataagg
      361 acacgggtga cctccggacc ctgatgggag tggtctgaat ctgcataaac ctcaattcca
      421 agaagtatcc tttcattgtg atttgtcatt gttacccgat agttcagaag ccagggtact
      481 gaaaaaaggg cctcatccat ttcaggaagc gtaattgttt ggcccatacc caaaaacact
      541 ctgccatccc accttcctct gacgcgttcc atccttctca aaaccgtccc gcagggacaa
      601 ggttcacgta tgaaccgggc tatatcccct gttctatacc ggatcagtgg cataccttta
      661 cgtgtcagcg ttgtgaatac aatttcccct gacgatccat catcaatgat ctctcctgtc
      721 tcagggttaa tcacctcaaa ataaagatct gcttcacgca ggtggtaccc attatgagca
      781 tcacactcaa cgcctccccc aaggcccatc tcggtcattc cataatgatt aaatactgag
      841 cagccccaga tttcttcgag gcttttaatg attgattggg gaacataatc agtgcttaaa
      901 agaacgttct tgatcattcc ttttttcaaa acagccccat ccctgctcct tgcaagggca
      961 aggacctggg tggggattcc cacgagagag tcgatctcaa gactaacaat tttatctatc
     1021 gcagttttta cgtcgtgtac aggtccatgg gcaaaactat caacctttat ccgtttgagc
     1081 ccccttgcca gcaattcacc cacactgtcc ggttgatctc ccggtaatag aatcagcact
     1141 ctttgacctg gtttaacaaa ggttgacata ccgatagcaa aaaagtcgat agtgagttca
     1201 aggtcttcct gtgtaaagaa aatgcgcttt ggcttcccgg tactccctga agtgcggagg
     1261 gtgacaattc tggttatatc ctcctgatgg acagttaaaa aatgccaggg gttatctgaa
     1321 aggtcggacg gcatcgtaaa tggaagttga ctcatgtctg cgagggtatt cagcggctcc
     1381 tcaggtacaa gcgccaaatg gcgagcataa aattgtgatt tttgacgggc ataattgatt
     1441 acatccttca ggcgctgaag gtgataattg tttagggcgt cctgaaaaga tctatccggt
     1501 gttgacagac ctatctcgat cttatttcta atccaaacct ctaatggtgt taaggagatc
     1561 atggtcatct tgattcatgc caggagcaat gtgggatcta ccctatcctg ccaatcaaag
     1621 tgtggacaga aaacaagcct gccatcatat gttggtgctg atatacagta tctcctgggc
     1681 tgttcaagat taaggctctc tgggttctga aatggtatga ctgatatagt gaaactttga
     1741 gtggtggcat catagagaaa aacctccaga ccaggacagc tttcttttct ggcaacccct
     1801 gtgcgtgttc ccttgcaata gaggcgtccc atcatatttg tgtctggttt tttttcattg
     1861 ttattcacct tttatggcag gcaggctgta acccatgatc tgcaaggata tccatgcact
     1921 tcttgtacgt ttcggagaca ataaggccgc acatactgcg gtcggggtac ttctccagaa
     1981 tctcatggca cctggtccct ccatactcct cgtgggctgc caaggtaaac cattccacga
     2041 ggtcaccaat catagttgtg caacggttat cctgtgtttc tccctcactg ccctttcctg
     2101 aataaagaga aatcagacaa gcacccccag acaaggcgcc gcaaacttcg ccgctccagt
     2161 taactccaaa acagagccca cccagggacc tgaccaggtc aggatttttt ttaccttctg
     2221 cttccattgc cagcataaca atgatttggg aacaacagaa gccttttgcg tacaatttca
     2281 ttatttgcag aaatatgttg tccatagtat gtaatctccg ttgtcatgga tttaataaaa
     2341 gtgcgtgaat tcaatcaaca tacctgtatt tttagtgaaa tggaaagaaa atactcagaa
     2401 taaaaccgca tcgaacgggc ttacgattta cttatcctca acgatttgtt ctccctcagc
     2461 catctcccct gtagccagtt cttcatcgat cagtatcatt ccgcaaaggg gacatacggg
     2521 aagatgaaca gcaaatatat tccccctata ctcaagtcta accgtttgaa cctccaaagg
     2581 gctttcgcat ccatggcaga tccacccttt atcacttcct gtcttgttca actctgtcat
     2641 ggtttgcctt ttctctttag ctccatccgg tgtgaatagg tggtgtgcac gtgataatct
     2701 tctccaacaa cactgtattc cacccaatat gtgactctat ccggccgaaa agatgcgaga
     2761 aagcgcccgg tggtaggatt gataaatttt tctccaatag tctcggcatg atggattgtc
     2821 ctctgtacat cctccttaag gatgcctcta tcattcataa gacgttccac ttcttcagaa
     2881 ataaacaggc ttatgtgctg ataagcattc ttctgttctg ctgtcatgtt attaatctgt
     2941 caaattgttt tttttgtagc ccatatcctg gaaacaattc aaaaaaagct ctcactttgt
     3001 taaaataatg ctaatcttgg gcaaatattt ttttcagata tcgatatata ccatgaatct
     3061 catgcctcga gtacctgtga aaccatcgtt gctatatctc atcatggtca atcaattgaa
     3121 tagctaccct atcccctgcc attacctttg taacagtttc cggaataatt atcagacaat
     3181 ttgcatcgct cattgaacga agcatggatg gtttctgatg tgatgttttt gaaacataaa
     3241 attcattatt tataatagtg tagcggcccc ttactaaatg gtgaatcttc cccgaagtga
     3301 tatcctgctc aagggatgcg ttaacaatcg gttttcttat cttggtcgca cccattaacc
     3361 tgagtaatgc aggcctgaca aattgaataa atgatgtgag ggcaggaaca ggatttccag
     3421 gcagaccaaa aaccatttta tcgccttttg ttccaaaaac acatggcctg ccaggtttga
     3481 tgttgactcg gtcaaagtgt atttcaatat tcagatcaga acaaacctct ttgacaagat
     3541 catactttcc agccgaaacc cctcccgtag atattataac gtctgatttc aaggcgttag
     3601 caaatatttt tctcatttca cccagtgaat ctttagcaat ccccagataa cgtgcatggc
     3661 caccatattt tgtcacctct gagcatatgg tgtaagcatt gctgtttctt atctgatgga
     3721 tttgaatttc ctcgcctgta tcagctaatt catcacccgt tgatataact gatacggtgg
     3781 gccgtttata tacttttact gtattgcggt ggagcgatgc aagaatgcct acatctgccg
     3841 aattcaaccg ttttcctttt gacaggatat gctccccttc ccttatgtgt tctcctgctt
     3901 ccctgtaatt ctcatatttt tctgtttcac agaataattt tatataacca ctttcttctt
     3961 ctgtatcttc aaagcggact acagaatcag caccttgcgg aatgggagca ccagtcatta
     4021 ttcttattgc tgttcctttt gagacttgat accctgtact ggaactgcct gcccttatct
     4081 ctcctaccac atgcagtttt accggacaat tgagagatgc gcctcgagtg tcatcagcga
     4141 ttattgcata accatccata gccgaatcag ctacaggagg catcattcta tccgaaacaa
     4201 catcttcata taatatccgg ttgaatgctc gcatgagagg aatattttcg aagctgagtg
     4261 ggttgacaga ccttaaaaca atttctaagg cttcttctac agatacagtc agatttttgt
     4321 tatgcataat atacaggctg gcggaactag atctctttga tcccatttta gttttctttc
     4381 ccctcaggct acctctttct ctttagcctt tcttaccaca cccc
//
LOCUS       DAOOMC010000175         4394 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdus sp. isolate AMR_MDS_2685
            NODE_23990_length_4394_cov_6.078590, whole genome shotgun sequence.
ACCESSION   DAOOMC010000175 DAOOMC010000000
VERSION     DAOOMC010000175.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864760
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdus sp. (wastewater metagenome)
  ORGANISM  Syntrophorhabdus sp.
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae; Syntrophorhabdus.
REFERENCE   1  (bases 1 to 4394)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4394)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,729
            Genes (coding)                    :: 2,708
            CDSs (with protein)               :: 2,708
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 7 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4394
                     /organism="Syntrophorhabdus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23990_length_4394_cov_6.078590"
                     /isolate="AMR_MDS_2685"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2699756"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..994)
                     /locus_tag="PK125_12860"
     CDS             complement(<1..994)
                     /locus_tag="PK125_12860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB39035.1"
                     /translation="MENQLVFDFFRTMNIARRNLIAYPRGHTLVTESFEKVLSVLVDF
                     FERSNSFTIGIAKDTLMVGTKALDKKNPVFQNFAHILFEHGIVSLTFLRGLTVDELMD
                     FDHIISQKRNDVQHQGGVEALLNKAKVSNIKVQLIDYKMFHAQEGLIETGANIDDFQE
                     SLFWWHFVRGVMDGTINPFGLPFGEMKEVDPENLAAIINNQFGGLGGLSAESSDGNES
                     VKDSSGEEENLPSSQSNKKDIGDERANEGTLATSGPGGEDSDGVNSTVKRPEFSAFLD
                     SKKFDFCKLAHDETSTGRLNRFVRSLAPELRKVFMERFLSSFTHNTKALNQIIPS"
     gene            complement(1245..1502)
                     /locus_tag="PK125_12865"
     CDS             complement(1245..1502)
                     /locus_tag="PK125_12865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB39036.1"
                     /translation="MRKRIIIGVQVTNRIKNALEIQEILTEFGCNIKTRLGLHEVTET
                     VCSTLGILILEMYGDIKQIKDMETRLKAVQGIVVKKMVFEE"
     gene            1740..1916
                     /locus_tag="PK125_12870"
     CDS             1740..1916
                     /locus_tag="PK125_12870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB39037.1"
                     /translation="MNISIDGGIVMKRGLFGGEEKPLSIQIGAKDTILSILVSLIENI
                     NEPQLLLTMELVII"
     gene            1913..2308
                     /locus_tag="PK125_12875"
     CDS             1913..2308
                     /locus_tag="PK125_12875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB39038.1"
                     /translation="MNTPSITSFSNRIAAPGFPTLHFDFLYNEYGREVPDKHKTLGQA
                     WWTARDCVKAEHGNTIDIWIAAGNFMNGTVDSLTVANNALPVCGFIFHGYPLHPAKLC
                     ETPLRTFGICCIDYNNRVNATGLPCEPTG"
     gene            2324..2776
                     /locus_tag="PK125_12880"
     CDS             2324..2776
                     /locus_tag="PK125_12880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HPB39039.1"
                     /translation="MADYRNELTEGERILYQAKLHWVLFLGPAMLIVLGGLWIPAKGL
                     AALVFVVIGLAWGVLSYRKYYNSYIHVTNKKVVICTSFLMKRPYRIPLADIGYVDFYQ
                     PSLGAILNFGKITIVHGDKYKSVFRMVSSPGNLVAVLKEQVALIPKNA"
     gene            complement(2792..3703)
                     /locus_tag="PK125_12885"
     CDS             complement(2792..3703)
                     /locus_tag="PK125_12885"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HPB39040.1"
                     /translation="MDIKQKTSLFAVITAFILASSKFSVGFISGSLAIISSSLDSLLD
                     TFMSGMNFFAIKKAGQPADDSHQYGHGKVEDLAAVAQSVVVVFSGVFIIYKAVESYMN
                     SVTITYSLYDFLVMIISLALSFAIVLLLRRIGKQTGSNALLADALHYTSDLFSNSGAI
                     LAIALTHYTGNIYYDLLFAIIIGCIIIFSAVKIARSGISGIMDSSISEKMTKEIERII
                     QEMPFPAVGFHKLRTRHSGSTKYIDFHMLFCKRLLIDEAHELANNLESEISREVKNAD
                     VVIHIEPCNYECDLTEATCTIRLGRKT"
     gene            complement(3785..>4394)
                     /locus_tag="PK125_12890"
     CDS             complement(3785..>4394)
                     /locus_tag="PK125_12890"
                     /inference="COORDINATES: protein motif:HMM:NF013916.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB39041.1"
                     /translation="SKTDLVKTSQSDPFSGEWYDFQQVIDFRDNSGLKILGIGYTIFL
                     GSKHEAKMLHQAAQLVYNAHQHGLISVLWIYPRGKAVSDEKDPHLIAGATGIALCLGA
                     DFVKVNYPQKEGMESKEIFKEAVQAAGRTKVVCAGGSSKDAKDFLQELHDQINISGAA
                     GNATGRNIHQKPLEDAIRMCNAVFAITVEGATVEEALKIYNN"
BASE COUNT         1243 a          934 c          922 g         1295 t
ORIGIN      
        1 ggcttgggat gatctggttc aatgcctttg tgttgtgggt aaaagatgaa agaaatcgct
       61 ccataaaaac cttcctgagc tcaggtgcga gagatctcac aaatctgttc aaccggccag
      121 tggatgtttc atcgtgggca agtttgcaaa agtcgaattt ttttgaatca agaaatgctg
      181 aaaactcggg tcttttcaca gtactgttaa ctccatcaga atcttctccg ccaggacccg
      241 atgtcgccaa agtgccttcg tttgcccttt cgtcaccaat atccttttta ttggattggc
      301 tggaaggtag gttttcttct tctccggaac tatctttgac agattcattt ccgtctgagc
      361 tttctgcgga aagacctccg aggccaccaa actggttgtt gatgattgca gccaggttct
      421 cggggtcaac ctccttcatt tcaccaaagg ggaggccaaa aggattaatg gtaccatcca
      481 ttaccccccg gacaaaatgc caccaaaaaa gggattcctg gaaatcgtct atgttggcac
      541 cggtttcgat aagaccctcc tgggcatgaa acatcttata gtcgattagc tgtactttga
      601 tattgcttac cttggctttg ttaagaaggg cttctacccc tccctgatgc tgcacatcat
      661 tccgtttctg ggagatgatg tgatcgaaat ccatcaattc gtcaacagta aggcccctca
      721 aaaaggtcag gctcacaatt ccgtgctcga aaagaatgtg ggcgaaattc tgaaacacgg
      781 ggtttttttt atctagcgct ttagtgccta ccatgagggt atcttttgca attccgatgg
      841 taaaagaatt gctacgctca aaaaaatcca caagaacaga gagaaccttt tcaaacgatt
      901 cagttacgag tgtatgcccc ctggggtaag caataaggtt tcggcgggca atattcattg
      961 ttcggaaaaa atcgaataca agttgatttt ccaatttgtt ctcaactgaa acttgtagtc
     1021 cgatgccgtt tgacactatc tacttccttc catagagttt tacccggact cataccgtaa
     1081 aggtgataaa aatcacacat ataccatata attcatcggt taaaacaaga aaaacctaaa
     1141 taaaataaag aaataagagg ggaatagcca ggtgtggaaa actcttgctg cacgacgaca
     1201 gctatgtgtc gaattttctc aattcaaaaa tacaatggag acaattactc ctcaaaaacc
     1261 attttcttga caactatacc ctgaaccgcc ttcagtcgtg tctccatatc ttttatctgt
     1321 ttaatgtctc catacatctc gaggatgagg atgccgagtg ttgaacaaac agtttcggta
     1381 acctcatgca aacccaatct tgtttttata ttacatccaa attctgtgag gatttcctgt
     1441 atttcaagag cattcttgat tctgttggta acctgcacgc caatgataat cctttttctc
     1501 attattgacc tccacaggta tcgctattga gtctcatttg atcactttat tgcataacct
     1561 ggcaatttgc taattattat ttaattatta gggtatagca agagaaatct tccactattt
     1621 tcaccgagcc aagttgagga aatcccaaca gggatcatcc tgccaggttg ggctgttatg
     1681 tgcccgataa aatagggttt ctcagtataa atttgaagtt ttgtaaaaaa gttattaata
     1741 tgaacattag cattgatggt ggcattgtca tgaaaagagg cctgtttgga ggtgaagaga
     1801 agccgttatc gattcagatc ggggcaaaag acactatttt gagtatcctg gtgtctctca
     1861 tagaaaacat caacgaaccg caattgttgt tgaccatgga gttggtgata atatgaatac
     1921 accatcgatc acatctttct caaatagaat tgctgctcca ggttttccga ccctgcactt
     1981 tgattttctc tataacgaat atggtcgaga ggtcccagat aaacataaaa cactaggaca
     2041 ggcatggtgg actgcacgtg actgcgttaa agcagagcat ggtaacacaa ttgacatctg
     2101 gattgcagca ggtaatttta tgaatggcac ggttgactcg ctaacggttg ccaataacgc
     2161 cctccctgtt tgtggattta tttttcacgg gtacccactc caccctgcaa agttgtgtga
     2221 gactccactt cgaacttttg gcatttgttg tatagattac aacaaccgtg tcaatgccac
     2281 gggtttgcca tgtgaaccta caggatgaaa ggcgaggaaa tatatggctg attataggaa
     2341 tgaactaact gaaggtgaac ggattctgta tcaggctaaa ttacattggg tgcttttcct
     2401 ggggcctgcc atgcttattg ttctcggtgg gttatggatt ccggcaaagg gactagccgc
     2461 ccttgtgttt gttgtaatag gtttggcatg gggtgttctc tcatatagaa aatattacaa
     2521 ttcctacatt catgtaacca ataaaaaagt tgtcatctgt acaagttttt taatgaaaag
     2581 gccctacaga atacctctcg cagatatagg atatgtagat ttctaccaac cctctcttgg
     2641 ggctatactg aactttggaa aaattacaat tgttcacggc gacaagtaca aaagtgtctt
     2701 tagaatggtg tcatcccctg gaaatttggt agcagtatta aaagaacagg tagcgcttat
     2761 acctaagaac gcataatcct gtcctgcttt gtcatgtttt tcttcctaaa cgaattgtgc
     2821 aggtggcttc agtaagatca cattcataat tacacggttc gatgtgtatc actacgtcag
     2881 cattcttcac ctctcttgag atttcgctct ccaaattgtt cgctagttca tgggcttcgt
     2941 caattagaag tcttttacag aacagcatat gaaaatctat atacttggta ctcccggaat
     3001 gacgggtccg gagtttgtgg aatcctacag ctggaaatgg catttcttga ataatccgtt
     3061 cgatttcttt tgtcatcttt tctgaaatgc tggaatccat aatacccgat ataccgcttc
     3121 tagcaatctt gactgccgag aaaataataa tgcacccgat gataatcgca aataatagat
     3181 cataataaat atttccggtg taatgggtta aggcgattgc aagaatagca ccggagtttg
     3241 aaaaaaggtc gcttgtatag tgcaaggcat cagcgagcaa ggcattcgat ccagtttgtt
     3301 tccctatccg tctgagcagt aagacaatag cgaagctgag tgccagggaa atgatcatta
     3361 cgaggaagtc gtacaatgaa tacgtaattg ttaccgaatt catatacgac tcaactgctt
     3421 tatagattat gaagacgcct gaaaacacaa ccacaacaga ctgtgccaca gctgcaagat
     3481 cttctacctt gccgtggcca tactggtggg aatcatcagc cggttgtcct gcctttttga
     3541 tggcaaaaaa gttcatgccg gacatgaatg tgtcaaggag gctgtccagg cttgatgaaa
     3601 taattgccag tgaacccgaa ataaacccaa ccgagaactt gctcgatgca aggataaacg
     3661 cagttataac ggcaaaaagg gatgttttct gcttgatgtc catattcgaa agacactgta
     3721 aaggctagga gagtaaatca aactcctttt tgtatctctg cctgtgatgc aacagaaaaa
     3781 agactcagtt attataaatt ttcaatgctt cttcaacagt cgcgccttca actgtgatgg
     3841 caaaaacagc attacacatg cggatagcat cctcaagggg tttttgatgg atgttcctgc
     3901 ctgttgcatt tccagctgca ccacttatat taatctggtc atggagttcc tggaggaaat
     3961 cttttgcatc tttactagag ccgccggcac acaccacctt tgtccggcct gcagcttgta
     4021 cagcttcctt gaatatttcc tttgattcca tgccttcttt ttgcggatag tttactttga
     4081 caaaatctgc accaaggcaa agagctatgc ctgttgcacc agctatgaga tgggggtctt
     4141 tttcatcgct cactgctttg cctcgtggat aaatccacaa tactgatatg aggccgtgct
     4201 ggtgtgcatt ataaacaagc tgggcagcct gatggagcat cttagcttca tgcttgctgc
     4261 cgagaaaaat ggtataacca atgccaagta tcttgagccc actgttatca cgaaaatcta
     4321 ttacctgttg aaaatcatac cactccccac tgaaagggtc agactgggag gttttaacca
     4381 agtctgtttt gctg
//
LOCUS       DAOOMC010000176         4374 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdus sp. isolate AMR_MDS_2685
            NODE_24124_length_4374_cov_6.111600, whole genome shotgun sequence.
ACCESSION   DAOOMC010000176 DAOOMC010000000
VERSION     DAOOMC010000176.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864760
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdus sp. (wastewater metagenome)
  ORGANISM  Syntrophorhabdus sp.
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae; Syntrophorhabdus.
REFERENCE   1  (bases 1 to 4374)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4374)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,729
            Genes (coding)                    :: 2,708
            CDSs (with protein)               :: 2,708
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 7 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4374
                     /organism="Syntrophorhabdus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24124_length_4374_cov_6.111600"
                     /isolate="AMR_MDS_2685"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2699756"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            319..1056
                     /gene="panB"
                     /locus_tag="PK125_12895"
     CDS             319..1056
                     /gene="panB"
                     /locus_tag="PK125_12895"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009245056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HPB39042.1"
                     /translation="MLTSYDYAFASIVDQAGIDSILVGDSVGNTVLGYTSTIPVTVEE
                     MIHHTKAVTKGAKKALVVIDMPFMSYHESIEQAKRNAGRMIKESGADAVKLEGGTRMK
                     EVIKALVDIEIPVMGHIGLTPQSVNRMGGYKVQGKGKEAESLILDAKAVEEAGAFSVV
                     LECVPRKLAKEITEMLTIPTIGIGAGPDCDGQVLVIHDLLGLFGGFRPKFVKKYLDMR
                     EEMDSAIKNYIEEVRSGVFPDDSHSFH"
     gene            1019..1915
                     /gene="panC"
                     /locus_tag="PK125_12900"
     CDS             1019..1915
                     /gene="panC"
                     /locus_tag="PK125_12900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HPB39043.1"
                     /translation="MGSFRTIPTLSTNMRTITTIKEIQAVSKELRKDKKIGFVPTMGY
                     LHEGHLALVKKARDLADIVIVSIFINPTQFGPNEDLAKYPRDFDRDAILLEQEKTDII
                     FFPDSKEMYPKGYTTYVQVRDLQNQLCGKSRAGHFVGVATVVAKLFNIVMPHFAVFGQ
                     KDYQQLKIIERMVQDLNMDIEIIGHPIVREQDGLAMSSRNTYLSPEERSRALLLWASL
                     TEAERLFRNGTREAAIIQREVERVLKSKEGVDIEYVSITDPETLEDVIQIEGKALVAV
                     ACHVGKTRLIDNKVLTEEQQNA"
     gene            1908..2294
                     /locus_tag="PK125_12905"
     CDS             1908..2294
                     /locus_tag="PK125_12905"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR00223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004068 - aspartate 1-decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006523 - alanine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HPB39044.1"
                     /translation="MHRRMMKSKIHRATVTDSNLHYEGSITIDETLMKMADIIPYEQV
                     DIYNVTSGERFHTYAIKGEKDSGVICINGAAAHKAKKGDIIIIVTYADFEEKELETFK
                     PKKVYADELNKIKSATGWSNRLSVAQ"
     gene            2782..3834
                     /locus_tag="PK125_12910"
     CDS             2782..3834
                     /locus_tag="PK125_12910"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HPB39045.1"
                     /translation="MYEQGVIRPPSEANSLLVRVTRNCPWNQCVFCPAYKGVKFSKRT
                     VEEIKKDIDQMAGEYGGYSFVKSVFLQDADSLLLSTPDLLEILRYLKQKFPDIQRVTS
                     YARAKTLKRKTVEELKELKEAGLTRIHVGMESGSEKVLKLIKKGITQADIVEGGRRVV
                     DAGISLSEYIMPGIGGKSMSEEHARETARLLNAVQPDFIRVRTFAMHPASPMQKMVEE
                     GTFVPMTDEEIVQEIKLLVSCLDKMHSYFSCADFSLNLLMQVDGYLDEKKDWMLEELD
                     RYLALTEMQKKAYSLLRRSTYMQYPIDAVNNEGLMKQLEHEIEKLEKSGKDGFNKYIQ
                     LLMSYQLPQPQTDSWS"
     gene            complement(3849..>4374)
                     /locus_tag="PK125_12915"
     CDS             complement(3849..>4374)
                     /locus_tag="PK125_12915"
                     /inference="COORDINATES: protein motif:HMM:NF012774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HPB39046.1"
                     /translation="PMVIAKLFRHVILAFFALLLCFSAANAFNKATVIKVVDGDTLKI
                     EMNRQEEAVRLIGIDTPESRINKKAKKDSLRSNQDIDTITAMGKEAARYVRTLVRKGD
                     MVGIEFDVQKRDKYNRLLVYAYLSDGKMLNEEIVKAGYANIMTYPPNVRYQDRFVRAY
                     REARDSRRGLYRDL"
BASE COUNT         1323 a          879 c         1029 g         1143 t
ORIGIN      
        1 gacgaaagcg agcgatgtga aacaccggag caaacgagcg tgtttgaggg gaatgctcaa
       61 cgcagacgct catccaatta gaaaatattt acgcctgata aattcattaa aaaataatta
      121 ttgacatata tcaacgagtg cctttatact tttcctgtgc ataattcgct tggatccaaa
      181 tactggaccg agacggaggg atagagagat tattaaacct tctgaaccgt gttcagaggg
      241 tttttttatt ggggattggg attggaaaaa gtaacagcac ctatattcag ggagaggaag
      301 ggcaaggaga agctaacaat gctcacttcc tatgattatg cctttgcaag tatcgttgac
      361 caggcaggca ttgacagcat tctggttggg gattctgttg gaaacactgt cctgggttat
      421 accagcacga ttccagttac cgttgaagaa atgatccacc acacgaaggc agtgaccaag
      481 ggggccaaga aggcactcgt tgtcatcgac atgccattta tgtcctacca tgaaagcatt
      541 gaacaggcga agagaaacgc cggcaggatg atcaaggaaa gcggcgctga tgctgttaaa
      601 ctggaaggcg ggacaagaat gaaagaggtc atcaaagccc tggttgatat cgaaatacct
      661 gttatgggcc acatcgggct aacgcctcag tcagtaaaca ggatgggtgg ttacaaggta
      721 caggggaagg gcaaggaagc agaaagcctt atcctcgatg ccaaggcggt agaggaagca
      781 ggcgcctttt ctgttgtact tgagtgtgtt ccgagaaagc ttgcaaaaga gataactgaa
      841 atgctcacta ttcctacaat tggaataggc gcagggccgg attgtgacgg ccaggtgctt
      901 gtgatccatg accttcttgg tttattcggg ggcttcagac caaaatttgt caaaaaatac
      961 cttgatatgc gtgaggagat ggactctgca atcaaaaatt acatagagga ggtgaggagt
     1021 ggggtctttc cggacgattc ccactctttc cactaacatg agaacaatta caaccattaa
     1081 agaaatacaa gccgtatcaa aagaactgag aaaggataag aagattggat ttgtaccaac
     1141 catggggtat cttcatgaag gacaccttgc ccttgtgaag aaggccaggg atcttgcaga
     1201 catagttatt gtaagtattt ttatcaatcc aacccagttt gggccaaatg aggaccttgc
     1261 gaaatatcct cgggactttg acagggatgc aattttgctg gagcaggaga aaacagacat
     1321 tatcttcttc cctgacagca aggaaatgta tcccaagggc tacactacct atgttcaggt
     1381 aagagacctc caaaatcagt tgtgtggaaa atctcgggct ggtcatttcg ttggtgttgc
     1441 cacagtcgtt gcgaaactct tcaatattgt aatgcctcat tttgcagtct ttggtcagaa
     1501 agactaccag cagttgaaga taattgagcg catggtccag gatctaaaca tggacataga
     1561 aattattgga catccaattg ttcgcgagca ggatggtctt gctatgagtt cacgaaacac
     1621 atatctgagt cctgaagagc gttcgcgagc attactgtta tgggctagcc ttacagaagc
     1681 tgaacggctg ttccggaatg gaacaagaga ggctgccata attcagcgcg aggtagaacg
     1741 tgttctcaaa tcaaaagagg gtgttgacat tgaatatgtg agcataacag atcctgaaac
     1801 gcttgaggat gtgattcaga tagagggcaa ggcccttgtt gccgttgcat gtcatgtggg
     1861 aaaaactaga cttatagata ataaggtttt aacggaggaa cagcaaaatg catagaagga
     1921 tgatgaaatc aaagatacac agggccacgg ttacagatag caaccttcat tatgaaggaa
     1981 gtatcaccat tgatgagacc ctaatgaaga tggcagatat catcccttat gagcaggtag
     2041 atatctacaa tgtcaccagt ggcgaacgtt ttcatacata tgctataaaa ggcgagaagg
     2101 attcaggggt tatatgcatc aatggcgcag ccgctcataa agcgaaaaaa ggggatatta
     2161 tcatcattgt cacctatgcg gatttcgaag aaaaagaact tgaaaccttt aaaccgaaaa
     2221 aagtgtacgc agatgaactg aacaaaataa agtctgccac aggatggtca aacagactca
     2281 gcgttgccca gtaaatacct tcgctgttat acatcgttag gaggcttttc tttactctca
     2341 ttatccctct ttagcctttc ctttaccttt tcaagtagcc tctttgccca gataaattca
     2401 gtagcaagaa tagcgagtcc tgctggtatg aagataaaaa gccggtcctg gcaggacaac
     2461 catggcaggc ccagtaatta tcattgaaaa accaatgagc gcaatgaaca ttttctttac
     2521 ctgatggaga gtttccatag ctacattgtt taaaattata tgttaaaaat gtaatcagga
     2581 acaaggtatg tgaaaattgc tcatcctgat ttctcttaaa gcgtaaacaa taaggctttt
     2641 ttcaggtaat tcattttcag caaccctggt ctacgagaag ctgccattgc aatgtttcag
     2701 tgctttggct tgtcaatcaa ggaagaacga cttatattct tcctgtattt cttgtaaatc
     2761 attgaaccaa aggagaaaca aatgtatgag caaggcgtaa taagaccccc aagtgaagca
     2821 aacagccttc ttgttcgagt gacaagaaat tgcccgtgga atcagtgtgt attttgtcct
     2881 gcctataaag gtgtaaaatt ttcaaaacgc actgtagaag agataaaaaa agatattgac
     2941 cagatggcag gcgaatatgg cggctattcc tttgtcaaat cagtttttct gcaagacgca
     3001 gacagtcttc ttctatcaac acctgatctg ctcgaaatac ttcggtatct gaaacaaaaa
     3061 tttcctgaca tccagcgtgt cacctcgtat gccagggcaa agacgctgaa gcgcaagacg
     3121 gttgaagaat taaaagagct taaggaagct ggcctgacgc gtattcatgt cggcatggag
     3181 agcggttcgg aaaaggtact caagctgatt aaaaagggaa taacacaggc tgacatcgta
     3241 gaaggtggca gaagggttgt agacgccggc atttcgctct cggaatatat tatgccggga
     3301 attggtggaa agagcatgag cgaagagcat gccagggaga cggcaaggct cttgaatgcg
     3361 gtacaacctg actttataag ggttcgaact tttgctatgc atcctgcatc tcccatgcag
     3421 aaaatggttg aggaaggaac atttgttcca atgacagatg aagagattgt tcaggagata
     3481 aagcttcttg tctcgtgcct tgataagatg cacagttatt tttcctgcgc agatttcagc
     3541 cttaacctcc ttatgcaggt tgatggttat cttgatgaga aaaaagattg gatgcttgag
     3601 gaactcgaca ggtatcttgc cttgacagaa atgcaaaaga aggcttactc gctcttgcgc
     3661 cgttcaacct acatgcagta tcctattgat gcggtcaaca atgaggggtt gatgaagcag
     3721 ttagaacatg agattgagaa actggaaaaa agcggcaagg atggttttaa caaatatata
     3781 caacttctca tgtcctacca gctaccgcag ccgcagaccg atagctggtc ttagaaaatg
     3841 cacgattgtt acaggtcgcg ataaagacca cgtctgctgt ctctagcctc cctgtatgcc
     3901 ctgacgaatc gatcctggta tctcacgttc ggaggatagg tcattatgtt ggcatacccg
     3961 gccttgacaa tttcttcatt cagcatcttt ccatctgata aataagcgta cacgagaagt
     4021 ctgttgtatt tatctctctt ctgaacatca aactcaatgc caaccatgtc accctttctc
     4081 acaagggtcc ttacatacct tgcagcttct ttccccatgg ccgtaatggt gtcgatatcc
     4141 tggtttgatc tcaacgaatc tttttttgcc tttttattaa ttctgctttc tggtgtatca
     4201 atcccgataa gccgtacggc ttcctcctgc ctattcattt cgattttgag tgtatcgcca
     4261 tccactactt taattacagt ggctttgttg aatgcgttag cagcagaaaa acacaacagc
     4321 aaggcaaaaa aagcaaggat gacgtggcgg aataactttg ctattaccat cgga
//
LOCUS       DAOOMC010000177         4159 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdus sp. isolate AMR_MDS_2685
            NODE_25785_length_4159_cov_5.976608, whole genome shotgun sequence.
ACCESSION   DAOOMC010000177 DAOOMC010000000
VERSION     DAOOMC010000177.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864760
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdus sp. (wastewater metagenome)
  ORGANISM  Syntrophorhabdus sp.
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae; Syntrophorhabdus.
REFERENCE   1  (bases 1 to 4159)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4159)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,729
            Genes (coding)                    :: 2,708
            CDSs (with protein)               :: 2,708
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 7 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4159
                     /organism="Syntrophorhabdus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25785_length_4159_cov_5.976608"
                     /isolate="AMR_MDS_2685"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2699756"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(509..1678)
                     /locus_tag="PK125_12920"
     CDS             complement(509..1678)
                     /locus_tag="PK125_12920"
                     /inference="COORDINATES: protein motif:HMM:NF002806.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HPB39047.1"
                     /translation="METIYFDHISGTTIHPEVKKTMINYINEHYGNPVSQHQLGDAAS
                     AALEAARQQVARLINAQPGEIVFTSGGTESVNHAIKGVAMATHDKGKHIITSNIEHQS
                     VSKSLRMLMRMGYRVTSLSVDKYGVVNPKDVERAITKETVLVTIMHANNEIGTLEPIA
                     EIGKITREKGVIFHTDAIASVGVIPFDVDEMGADLVSMAANQFYGPSGVGALYIRPKT
                     RILPIIEGGMQENGQRAGSENMVGIIGMGKAAELAKREMPERIKHLSQLRQAWIERLT
                     TIEAIDINGHPTNCLPHLVSFSVNFVEGESMILMLDEKGICASTRSACASGSLRASHV
                     LIATGCDYAAAQGTLIFSFGITNTMEELDKAIAVLKDSVVFLRNMSPLYKKRAAS"
     gene            complement(1854..2228)
                     /gene="nifU"
                     /locus_tag="PK125_12925"
                     /pseudo
     CDS             complement(1854..2228)
                     /gene="nifU"
                     /locus_tag="PK125_12925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331707.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold protein NifU"
     gene            2381..3916
                     /locus_tag="PK125_12930"
     CDS             2381..3916
                     /locus_tag="PK125_12930"
                     /inference="COORDINATES: protein motif:HMM:NF015558.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HPB39048.1"
                     /translation="MAENKKKIDFTTLWKKDDWMSVWIGFFILIIFMAGATFKLPGWK
                     WMTDGAFFEKSVTLPAKAEALAKEAEEKGDLGVQAAVMSLKTALEGKDRKAIGTAAGA
                     LEKAAKDAKDKDIKKKAEKMGKDIKSDAGNTAGKVFSGENMTRAFWLLVGMWVLGGIG
                     MAIMGLGFGKFTLGFPIVFILSALAFFVSGNSVVNYYGLEVVFWALIFGLIISNFLGI
                     PEWLKTAIKTEFFIKIGLVLLGAEVLFTTIAKVGAYGMIQSIIVIVAVFYVCFWVAKK
                     LGLDDEFASILGTAVSICGVSAAIAAGGAVKGDQKKISHTISLVLLCAIPMLLFQPLI
                     AKAVGMLPAVAGAWIGGTIDTTGAVVAAGAIAGEAAMAVAVVVKMAQNVLIGFAAFFL
                     AIWFTFKGQAAGEKPSLMEIWFRFPKFVVGFIIASIVFSFFMSEASAKAVTGITAGLR
                     GWWFTMAFLCIGLETKFKELVAMGGGRPAAAFLIAQVFNIIWTLIFAYLIFGGVLFPV
                     PKF"
BASE COUNT         1141 a          829 c          948 g         1241 t
ORIGIN      
        1 ataaaaatca tatatataaa atcaaaataa taataaaaat ctgatataat taatattata
       61 ttaatcgata ataattaaat ataataatat tataaataga aaaaatatca ataataatat
      121 aaaatatata ttatgtaata cataataaaa atccaagaaa ataattttta tggaaaatgg
      181 cagatttgca gtaaattatt gccgaataga caaaatggcc taagtaaaac ttaaagggta
      241 aacagtcgaa aagttaagaa ttaaggagac tcaccatgtt tttcgtgaag gttatggaga
      301 aaacgttaaa agttgaatag gggagaaatt aagaaaatgg aggtttcact actgtttata
      361 ttcacgattt ccctatttgc caaggtctca atttctgcct gagaaggata ggctgcgcaa
      421 gctcattatt caataaaaaa aggggatgtg gcatgttttc agtcccacag attttttgtt
      481 aactctcagc tttaaaaact cagccttgtt atgaagctgc tcttttctta tacagcggag
      541 acatatttct caggaaaacg acactatctt taagcactgc tatagcctta tccaactcct
      601 ccatagtgtt tgtgatgcca aaagagaata tcagagtacc ctgtgcagca gcatagtcac
      661 accctgtggc gataagaaca tgtgatgctc gaagcgaacc cgaagcgcat gctgaccgag
      721 tcgatgcgca gatccctttc tcgtcaagca tgagtatcat tgattcacct tcaacaaaat
      781 tgaccgaaaa ggagacgagg tggggaaggc aattggtagg gtggccgtta atatcaatag
      841 cctcaatcgt tgttagtctt tcaatccatg cctgccgaag ttggctaagg tgttttatgc
      901 gctccggcat ttcacgtttg gccagttcag ctgccttgcc cataccgata atgcccacca
      961 tattttcaga gccagccctt tgtccatttt cctgcattcc accctcgatg attggaagaa
     1021 tacgagtctt tggtctgatg tataaggcgc ctacaccaga aggcccataa aactggttgg
     1081 ctgccatgct cacgaggtca gcacccattt cgtcgacatc aaagggaata acccctactg
     1141 aggcgattgc gtcagtatga aagataaccc ctttttctcg tgtgatcttg ccaatctcag
     1201 cgatgggctc aagggtgcca atctcattat tggcgtgcat gatagtaacg aggaccgttt
     1261 ctttcgtgat cgcacgctct acgtccttcg gattaacgac accgtattta tccacagaca
     1321 gagaagtgac acgataaccc attctcatga gcattctcaa agatttagag acagactggt
     1381 gctctatatt ggatgtgata atgtgtttcc ctttatcgtg agtggccata gccacacctt
     1441 tgatagcgtg attgactgac tccgtaccgc ctgaagtaaa gactatctcc cctggttgtg
     1501 cattgatcag ccttgctacc tgttgtcgcg ctgcctctag cgctgctgat gcagcatcac
     1561 ccaactggtg ctggcttacg ggatttccat aatgctcatt aatatagtta atcattgttt
     1621 tttttacttc aggatgaatt gttgtgccag aaatgtggtc gaaatatatg gtttccatat
     1681 tcacctcttt tttggattgt aaaacattaa tcgtgatcgt gttcaactgt gggtttaccg
     1741 catctaacac agtgagcaac ctgttcgggc tgatcataat cacaataagg gcagtagcaa
     1801 tgtttatcgt gggagcttat gtgttctagt tcctcatcag gccttctacg gaccctgcct
     1861 tccttccggt caagataatc ctgtatagcc ttatgcaggg cgtcggcacc gaggtttgag
     1921 cagtgcatct tattttttgg aaggccaccg agttgatcag ccactgactt gttggtaatg
     1981 tgcatggctt cctcgatggt cttgcccttt gccatatctg ttaccatgct tgagactgca
     2041 attgcagccc cgcatccgaa agtctcgaat tttatatcat caatcttatc gtctttcact
     2101 ttaatcatga tcttcatcat gtcaccgcac acaggattac caacctgacc aactccatcg
     2161 ggattctcca ttgtgccaat gttgtgggga tttttgaagt gctccattac aatttcagag
     2221 tattgcataa taacctcctt tagcgtaaca ccttctccat tagtataaat cattttaatt
     2281 acagtgaaaa ggggtatgcc gttgacaaca cagaaaaatc gactatagtt ttacagtgga
     2341 tttatagatt ttaaaatcta tacttttagg gggtgttgtt atggcggaaa acaagaaaaa
     2401 aatagatttt accacgctct ggaaaaagga tgattggatg tcagtctgga taggcttctt
     2461 tatcttgatt atttttatgg caggggcgac cttcaaactt ccgggctgga agtggatgac
     2521 tgatggagcc ttctttgaaa aatctgtgac cttgccagcc aaggctgagg cacttgccaa
     2581 agaagctgaa gaaaagggtg atctaggcgt tcaggcagct gtcatgagcc tcaaaacagc
     2641 acttgaggga aaagatagga aagctatagg tactgcagcc ggagcattgg aaaaggcggc
     2701 aaaagacgct aaagacaaag acatcaagaa aaaagcagaa aagatgggga aggatataaa
     2761 aagtgatgcc ggcaatactg caggcaaggt tttttccggt gaaaacatga caagggcctt
     2821 ttggctcctc gtgggaatgt gggttctcgg cggcatcgga atggcaatta tgggcctggg
     2881 ttttggaaaa ttcactcttg gattccccat cgtctttatt ctctctgcac ttgcattttt
     2941 cgtgtcaggg aactcagtgg tcaattacta tggacttgag gttgttttct gggctttgat
     3001 ttttgggctc ataattagta actttctggg tattcctgaa tggctcaaga cagctatcaa
     3061 aacagagttt ttcatcaaaa tcggtcttgt tctactgggc gctgaagttc tttttacaac
     3121 tatagccaaa gtaggcgctt acggaatgat ccaatcgatc attgttattg tagctgtatt
     3181 ctatgtttgc ttctgggttg ccaaaaaact cgggctcgat gatgagtttg catcgattct
     3241 gggcacggca gtatcaatct gtggagtttc agctgctatc gcggcaggtg gcgcagtaaa
     3301 aggcgaccag aaaaaaataa gccatactat ctcgcttgtt cttctttgtg caataccaat
     3361 gttgttattc cagcccctta ttgctaaggc agtaggtatg cttcctgctg tggcaggcgc
     3421 ttggatcggt ggaacaatcg atacaaccgg tgctgttgtt gctgctggag caattgctgg
     3481 tgaagcagcc atggctgtag cagttgtggt gaaaatggca cagaatgtac tcatcgggtt
     3541 tgcagctttc ttccttgcta tctggtttac atttaaaggg caggcggcag gagagaagcc
     3601 gagtctcatg gagatatggt tcaggtttcc gaaattcgtc gttggattta taattgcttc
     3661 aatcgtgttt tcctttttca tgagtgaggc aagtgctaag gctgttacag gtattacggc
     3721 aggattaaga ggttggtggt tcaccatggc atttctgtgt attggtctcg agacaaagtt
     3781 taaggagctt gttgctatgg gtgggggcag acctgcagca gcatttctca ttgcacaggt
     3841 atttaatatt atttggacgt taatttttgc gtatctgata tttggcgggg tgttgttccc
     3901 ggtgccgaag ttctagtaat gttgtgttca gcgagtagct gacagctcaa aacagaataa
     3961 gagggaacga gcgaccgaat gaccgcttgc cctcttattc tgtttgtatg ttgattggcc
     4021 tcatcaacag gttcctcacg tcaatgacgc aggtggtatg tttatggata agggaaagat
     4081 aaggtgaggt agctactcat aacctttatt taccttcaag aaacccttaa caaaacctct
     4141 ctgtgcagta gagtagatt
//
LOCUS       DAOOMC010000178         4143 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdus sp. isolate AMR_MDS_2685
            NODE_25909_length_4143_cov_4.742661, whole genome shotgun sequence.
ACCESSION   DAOOMC010000178 DAOOMC010000000
VERSION     DAOOMC010000178.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864760
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdus sp. (wastewater metagenome)
  ORGANISM  Syntrophorhabdus sp.
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae; Syntrophorhabdus.
REFERENCE   1  (bases 1 to 4143)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4143)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,729
            Genes (coding)                    :: 2,708
            CDSs (with protein)               :: 2,708
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 7 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4143
                     /organism="Syntrophorhabdus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25909_length_4143_cov_4.742661"
                     /isolate="AMR_MDS_2685"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2699756"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(235..1269)
                     /locus_tag="PK125_12935"
     CDS             complement(235..1269)
                     /locus_tag="PK125_12935"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HPB39049.1"
                     /translation="MNVALVIKRALSRILYSSGLTDNILRRSDRGIIMCYHHVLREDD
                     ERIKFLQPGMYVTTKTFDLHIRYLSNHYQFTRLESLMDFHGCNKICAITFDDGWADNY
                     EFAYPILKKYRVPATIFIATNYIGSCKWPWPDRLSYYIHKSSGHEMAAINRIVNDHFP
                     EITKNIILVEKCNKNKLTSVEKIVSFVKSKSYDEISSFMEKVDSYMQHHLKNLEMYHP
                     WLTWKQIKEMSADEISFGAHTHNHVILSSVSLNEAKDEIYLSKKILSQQTGKPITSFS
                     YPNGFYHQGIVDILQMLEFKKAVITEVGTINSSKSMFELKRFLLHNDISDCIPMLAYS
                     LSVKLLAEKL"
     gene            complement(1266..2225)
                     /locus_tag="PK125_12940"
     CDS             complement(1266..2225)
                     /locus_tag="PK125_12940"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HPB39050.1"
                     /translation="MKIFILIFSNYIVFICSVKKPSQFILLVDIWLFSIMFSSFNGIM
                     QGGKIWGNRWLKDENHISLVAAMVIPFAFMLYRLYKPLLRKIFYVVCLVFYVSVNVVA
                     ASRGGALAMIVVAFLCWLLTSHKLRNTLIILLATVLVLSFAPPVFFEEMATLKQGTQE
                     GTASDRIYLWGIAMDMFEDHPFLGVGPMNYPYYFSSYEKGVIYPIGASRVAHSTPVQY
                     LAEFGALGSFLFLYFLYALYRNWRFAHKRDLSASRGVSYNEMHSYKLISHACGISQLG
                     FWIGALFLTLLPYPFYWCLVPISESWRKLIQENFKLRNEQVLM"
     gene            complement(2585..3811)
                     /locus_tag="PK125_12945"
     CDS             complement(2585..3811)
                     /locus_tag="PK125_12945"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HPB39051.1"
                     /translation="MLSSTIKRKLIIWQAWPEEDGWNILEACMVLQKDEDMKKHRIMF
                     VTHDLAIGGLQQVVVNLCRTIDKGVFEPSVLCLRSRGGFADEVEEMGIRVFLLPSKGG
                     RVDYVPYFRIARIFREEGIGIIHTHNTQPFVDGTFGALLAGVRTIVHTDHARDFPDKK
                     RYMFLEWLVSHFAYRVVGVSDHTTRNLIRYEKISAKKTMTIMNGIYGEKYRISIDTIK
                     KKMELGIRHDGPVIGLGVRLSEQKGITYLLQAMPDILKGFPELTLLIAGEGQLEDQLK
                     TEAKDRGISDHVIFAGPRLDMPEILKVLDLYVLPSLWEGLPMVLLEAMAAECPIVATD
                     VGGNSMAIQNGVNGILVPAKSSVELAKAVIGLLSDEQTRHAFIQKGREIFDKKFSARI
                     MTANYEQIYLRRELSF"
     gene            complement(3759..>4143)
                     /locus_tag="PK125_12950"
     CDS             complement(3759..>4143)
                     /locus_tag="PK125_12950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB39052.1"
                     /translation="LVKSWFFTADADNVEEFNRLLCEANQERLERERGVCFVSTHFGK
                     GYVKGSRLHQDTAALLKTLSLRNGWFVPVSTALDYIRSRQDQESLTVVQRIVLEARWF
                     LHTIRRRRGALQYDKTEIDYLAGVA"
BASE COUNT         1231 a          949 c          782 g         1181 t
ORIGIN      
        1 tacctgttaa ccttgtccat aagttaactt atagcacctt tcacgtgcta aagttgtcat
       61 gaaccttata ttattactga atctgttgaa aaaccctggt aagctcaaaa actatcaaat
      121 tttgaacatc aaaacactaa ccttggtcaa aatatttcaa atgtcatgca ttctgtgaag
      181 ccatagtttc tgccactctt catccatttg gtttttcaac agtttcactt gttatcatag
      241 tttctcagca agaagcttaa cagacaatga atatgcaagc attggaatac aatccgaaat
      301 atcattatgg agtaggaatc tttttaattc aaacatactc ttagagctgt ttattgtccc
      361 gacttctgta ataaccgctt tcttaaattc taacatttga aggatatcaa caatgccttg
      421 atgataaaat ccatttggat agctgaatga ggttatcggt tttccagtct gttgagataa
      481 tatttttttt gataaataga tttcatcttt tgcttcattc aatgaaacac ttgaaaggat
      541 aacatgatta tgagtatgag cgccaaaaga tatctcatca gctgacattt cttttatttg
      601 tttccaggtg agccaaggat gatacatctc cagattcttt aaatggtgtt gcatatatga
      661 atcaactttt tccataaatg agcttatctc atcataactc tttgatttga caaatgacac
      721 aatcttttct actgatgtca acttgttttt attacatttc tctaccaaaa taatgttttt
      781 tgtaatttca gggaagtggt catttacaat tctatttata gcagccatct cgtgccctga
      841 agatttatga atataatagc ttaaacgatc gggccaaggc catttacatg agccgatata
      901 atttgtagca ataaaaattg tagcaggcac tctatatttc ttcaggattg ggtaggcaaa
      961 ttcataatta tcagcccacc catcatcaaa ggtaatagca catattttat tgcagccatg
     1021 gaaatccatc aaactctcta acctagtaaa ttgataatga ttagatagat atcgaatatg
     1081 caaatcaaag gtttttgttg ttacatacat acctggttga aggaatttaa ttctttcatc
     1141 atcctcccgg aggacatggt gataacacat tattattcca cgatcagatc gacgcaggat
     1201 attatctgtc aatccggagg aatatagaat tcttgataaa gctcgtttta ttactaatgc
     1261 aacattcaca ttaatacctg ttcattgcgt aatttgaaat tttcttggat caattttctc
     1321 catgactcac ttatcgggac aagacaccag taaaatggat acggcagtaa cgtcagaaac
     1381 aaggcaccaa tccagaagcc caactgagag ataccgcatg catgagagat gagcttataa
     1441 gaatgcatct cgttataaga tactcctcgt gatgcactca aatctctttt atgggcaaat
     1501 cgccaatttc tatacagagc ataaaggaag tacaagaata gaaaagatcc tagtgcgcca
     1561 aactctgcaa gatattgtac aggcgtcgaa tgggcaacac gagatgcacc aatgggatat
     1621 ataactcctt tctcatatga agaaaaataa tatggatagt tcattgggcc gacacctaaa
     1681 aaggggtgat cttcaaacat gtccatagcg attccccata gataaatcct gtcggatgcc
     1741 gttccctcct gcgtaccctg cttgagagta gccatctctt caaaaaatac cggtggggca
     1801 aaactcaata ccagtaccgt cgccaacaag ataatcaggg tattcctcag tttatgactc
     1861 gtcaacaacc agcataagaa agctacaact atcatcgcca aggctccgcc acgagaggca
     1921 gcaacaacat tgacagacac ataaaaaacc aggcaaacaa catagaatat ttttctcaga
     1981 agaggcttat aaagcctgta aagcatgaag gcaaacggga tgaccatggc cgcaactagg
     2041 gatatgtgat tctcatcttt gagccatctg ttgccccaga tctttccacc ctgcataatc
     2101 ccattaaaac tgctgaacat tatggaaaag agccagatat caacaagaag tataaactga
     2161 gatggtttct tcacactaca aataaagaca atatagttcg agaaaatgag aataaagatc
     2221 ttcaccaaat cgatccttgc cacctccaat tttgttgaca aaagggtata caatataatg
     2281 aaagcacata ggagagcaaa tctcctagtt gtcatggacg accaatccac ctggttggat
     2341 gcaatcaaat acaaggcata aaacatggtt agatatgata cgatcgctgg aattttcaat
     2401 tcgtttattc cctgatagag cccttgtatt gatccaagct cgaatagaaa gtatatgctg
     2461 ataaggatta ttcccggatt cacatcttgc catcggggaa aattttccgc aaacacagaa
     2521 tctagagaaa taggctgatc ttctgtataa gacattattg tatcatcctg catcatcatg
     2581 atatctaaaa tgataattct cttcttaggt atatctgctc atagtttgcc gtcatgatcc
     2641 gggcactgaa tttcttgtca aaaatctctc tccccttctg aataaaagca tgccttgtct
     2701 gctcatcact gaggagtccg atgactgctt tcgccagttc gactgaagac ttcgcgggga
     2761 caagaatgcc attgacacca ttctgtatcg ccatggagtt tcctcccaca tctgtagcca
     2821 cgatgggaca ctcggcagcc atggcctcaa gcaacaccat gggaagtcct tcccagagag
     2881 acggaaggac atagagatcg aggaccttga gaatttcggg catatcaagc cgaggtcctg
     2941 caaatatcac atggtcactt atcccacggt ctttcgcttc agttttcagc tgatcctcta
     3001 gttggccttc tccagcaatg agcagggtaa gctctgggaa gcccttcaat atatctggca
     3061 tggcctggag cagataggtg atccctttct gttcagacaa tctcacgccg agcccgataa
     3121 ccgggccatc atgtcttatt cccaattcca ttttcttttt tattgtatca atggatatcc
     3181 tgtacttttc tccgtagata ccgttcatta tcgtcattgt ctttttcgcc gagatcttct
     3241 cataccggat aagattacga gtagtgtgat ctgatacacc caccactctg taggcaaagt
     3301 gagagacaag ccattcgaga aacatatagc gcttcttatc gggaaaatcc cgtgcgtgat
     3361 cggtatgtac tattgtcctc acgcctgcca ggagcgctcc gaatgtcccg tccacaaagg
     3421 gttgcgtgtt gtgggtgtgt ataattccta ttccttcctc tctgaatatt cgggctatac
     3481 ggaaataggg aacgtaatca accctgcccc cctttgatgg aagaagaaac accctgattc
     3541 ccatctcctc aacctcgtcc gcgaaccccc ccctggatcg caggcacagg actgacggct
     3601 caaagacacc tttgtcaatg gtccggcaga gattcaccac cacctgctga aggcccccta
     3661 tggcgagatc gtgggttacg aacatgatgc ggtgtttttt catatcttca tctttctgca
     3721 ggaccatgca ggcttcaagg atattccaac catcttcttc aggccacgcc tgccagataa
     3781 tcaatttccg ttttatcgta ctggagagca cctctcctgc gacgaatagt atggagaaac
     3841 catcttgcct cgagaacaat acgctggaca acggtcaaag attcttgatc ctgacgactt
     3901 cggatgtaat caagggccgt ggaaactggg acaaaccacc cgttcctgag actcagtgtt
     3961 ttcaggagcg cagctgtatc ctgatgaagc ctgcttcctt tcacatatcc cttgccgaag
     4021 tgcgtgctca caaaacagac ccctctctcc cgctccagcc tttcctgatt tgcctcacag
     4081 aggagccggt tgaactcttc gacgttgtca gcatccgcgg taaagaacca ggacttgacc
     4141 aag
//
LOCUS       DAOOMC010000179         4104 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdus sp. isolate AMR_MDS_2685
            NODE_26242_length_4104_cov_6.785873, whole genome shotgun sequence.
ACCESSION   DAOOMC010000179 DAOOMC010000000
VERSION     DAOOMC010000179.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864760
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdus sp. (wastewater metagenome)
  ORGANISM  Syntrophorhabdus sp.
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae; Syntrophorhabdus.
REFERENCE   1  (bases 1 to 4104)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4104)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,729
            Genes (coding)                    :: 2,708
            CDSs (with protein)               :: 2,708
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 7 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4104
                     /organism="Syntrophorhabdus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26242_length_4104_cov_6.785873"
                     /isolate="AMR_MDS_2685"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2699756"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1237)
                     /locus_tag="PK125_12955"
     CDS             complement(<1..1237)
                     /locus_tag="PK125_12955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB39053.1"
                     /translation="MKKRNLPWLMGVVLAGLAILVFTTVSFVSCKKSSPKPEKKPQQH
                     LFSITPGDGEIIRHLSQPIFVSFRQPVQPSDFIFKIEPDPGGWEVIWDKSQKRVRLDH
                     KNPFIPQQQYHIKLSIKDGSLESQFKTLSPDPCNLLASDLALGVITPDEAAQYHIMRI
                     FNPKSVPKKYRYAKAPRSGTWDILQAANLRDKLKPETVKTLEPYMVRPDNPKSFFYKR
                     LYGLGQSSSRALPWSIAMAHASGAKEPFKEVYQTDTGYTIEIFGEPGLGKMVQKARRL
                     VEEHKMYERFEKLLNRKTADFGDKTLRIYIFSGLSEMEDEAGYKSEPKGYCEPLTWDP
                     ALNTGAGTATTDIVISARQVSPDPDLAATLAHEMFHSFQFAFSRFEQKWLMEGSAVWA
                     EDFIGHDWNTEQKWLAGYTF"
     gene            complement(1330..2184)
                     /locus_tag="PK125_12960"
     CDS             complement(1330..2184)
                     /locus_tag="PK125_12960"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HPB39054.1"
                     /translation="MKRYKASICWIIAVFILSLSSLVFAQEDRKGCKDHPLFSRMQGY
                     RIAVCRDREFDAEDFIDPATQQKVTIEGRKFHTEYSALKPFEGKYSRLQVSRNYTNAI
                     TKIGGIAYEKNPKDPSETSMKLIKDDKEIWAKLQFNFGANYLYLTIVEKQAMAQQVVA
                     DARFMAEGINTTGHVALYGIHFDFNKADVKPESEPALKEITILLQKDPRLKIYVVGHT
                     DNAGGLDYNMKLSHQRAEAVVKELISKYKIAPDRLKAGGVGPLAPVASNDTEEGKAKN
                     RRVELVKQ"
     gene            complement(2280..3392)
                     /locus_tag="PK125_12965"
     CDS             complement(2280..3392)
                     /locus_tag="PK125_12965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB39055.1"
                     /translation="MKRKVIFFLVLIAWAGSLPQVLADSWPKLIGGKTSIQCNEALRI
                     ADALFQSDASVLYAPPVIPSDLKSVLVLGPDGLDISGGDAIKIDHTEFDKIPISEEKA
                     SRSIYWQKKPGGRYRLVVRENPVGWRGDMYSLYITENKIEPKMFLAEINKTRGNSGFE
                     PVISDTWRPPLVFRSKVSGELWFIEVGEPYQFFADWRVYGVDTTGAKSLCTVRFRPQV
                     HSAKYLLPQPVQRLAVLLDQTIGQDKDEGTLQPTARLRMDVEWTWANAALRPWALKEK
                     YNTRDEVDASLKKWTQKGHSYQKHYQAIQQQYTEAERSLTEYYKTTFRKSPRDAKELA
                     IYALDIAYRSYYTFQRDDPNNYFRLEEVNRNPWRKQ"
     gene            complement(3717..3935)
                     /locus_tag="PK125_12970"
     CDS             complement(3717..3935)
                     /locus_tag="PK125_12970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB39056.1"
                     /translation="MAKLLEKAFKEASKLPEVEQNAFAKWVMDELEAEAKWQETFASS
                     EDILDKLADQALAEHKAGKSKILNVGNQ"
BASE COUNT         1006 a          938 c          903 g         1257 t
ORIGIN      
        1 caaaagtata ccctgccagc catttctgct ctgtattcca atcatgacca atgaagtctt
       61 cggcccatac cgcggaacct tccattagcc atttctgttc gaatctactg aaggcaaatt
      121 ggaaggagtg gaacatttcg tgcgcgagag tggccgccaa gtctggatcg ggactcacct
      181 ggcgggcgga gataacaatg tcggttgtag cggtcccagc tcctgtgttt aaagctgggt
      241 cccaggtcaa tggttcgcaa taacccttgg gttcggattt gtagccagcc tcgtcctcca
      301 tttcactcaa acctgagaaa atataaattc ttaatgtttt atcgccgaaa tcagcagtct
      361 tgcgattcag gagcttttca aacctttcat acatcttgtg ctcttcgacc agcctgcgtg
      421 ctttttgcac cattttaccg agacctggtt ctccaaaaat ctcaattgtg taaccggtgt
      481 cagtttgata cacttcttta aaaggttctt ttgcccctga tgcatgggcc atggcaatcg
      541 accatggtaa tgccctggat gatgattggc ctaacccgta taaacgttta taaaaaaaac
      601 tttttggatt atcgggcctt accatataag gttctaatgt cttgacagtc tctggcttca
      661 gcttatcgcg gagattggcg gcctgaagta tatcccaggt gcctgagcga ggagccttgg
      721 cgtagcggta tttcttaggc acagattttg ggttgaaaat gcgcatgatg tggtattgag
      781 cagcctcgtc aggagtgata acgcccaggg ctaaatcaga cgccaagaga ttgcaagggt
      841 caggagatag cgttttgaac tgtgattcaa gagagccatc tttaattgac agtttgatat
      901 ggtattgttg ctgtgggata aatggatttt tatgatcgag ccgtaccctt ttttgagatt
      961 tatcccaaat tacttcccag ccgccgggat caggttcaat tttaaatatg aaatcagaag
     1021 gttgcaccgg ttgacggaat gaaacaaaaa taggctggct gagatgacga ataatttcgc
     1081 catcgccagg ggtgatggag aagagatgct gctggggttt tttctcgggt tttggagatg
     1141 atttcttaca tgaaacgaaa gaaaccgtcg tgaatacaag gatcgccaga cccgccagaa
     1201 ccactcccat cagccatggt agatttcttt ttttcattga cgtctttaag ttatgagcta
     1261 ccccacaaaa ctccttttag actgtggaga caaaatatga cattgtagag ttttggattc
     1321 ctcttaatct cattgtttca ccaactccac cctgcggttt ttggcctttc cctcttctgt
     1381 gtcattggaa gcaacagggg ctaaaggacc cacaccgcct gccttaagac gatctggcgc
     1441 aatcttgtac tttgaaatca attctttgac cacagcctcg gctctttgat gggagagctt
     1501 catattataa tcgagtcctc ctgcattgtc cgtatggcca accacataga ttttcagtct
     1561 gggatctttc tgaagcagta ttgttatctc tttcaaggct ggttcagatt caggtttgac
     1621 atcagccttg ttgaaatcga aatgaatccc atagagagca acatggccgg ttgtgttgat
     1681 cccttcagcc atgaacctgg catcggcaac tacttgctga gccatggcct gcttttccac
     1741 aatggttaga tagaggtagt tagctccaaa gttaaactga agcttagccc agatctcctt
     1801 atcatcttta atgagtttca tggatgtttc tgaggggtcc ttgggatttt tctcatatgc
     1861 aataccccct atctttgtga tagcatttgt ataatttcgg gaaacttgta gcctggaata
     1921 tttcccttca aatggtttaa gtgcactgta ttctgtatga aatttccttc cttcgattgt
     1981 caccttctgc tgtgttgcag ggtctatgaa atcctcagcg tcaaactccc tatctcggca
     2041 gacggcaatc cgatatcctt gcattcttga aaagagcgga tgatccttac atccctttct
     2101 atcctcctga gcaaacacca gagaactgag gctaagtatg aagactgcga tgatccaaca
     2161 gatagatgct ttatatcttt tcatgatctg tgcctcctta aattattgac taacccagaa
     2221 ttgggccgga tcgtaagtga tcggctccag cgactggtta tacccggttc tatgaatttc
     2281 tattgttttc gccatgggtt tctattgacc tcctctaatc tgaaataatt gtttggatca
     2341 tcacgctgga atgtatagta tgaccgataa gcaatatcta gcgcataaat tgccaactct
     2401 tttgcgtcgc gtggcgactt acggaaggtt gttttgtagt attctgttaa agaccgctct
     2461 gcctccgtgt attgctgttg aatagcctga tagtgtttct gataggaatg ccccttttgt
     2521 gtccactttt ttaaactggc gtcaacttca tcgcgggtgt tatacttctc tttaagagcc
     2581 cagggacgca aagcagcatt ggcccatgtc cattctacat ccattctcag tcttgcagtc
     2641 ggttgaagcg taccttcatc tttgtcctga ccgatggtct ggtctaaaag cacagcaagc
     2701 ctttgaactg gttggggcag caggtatttg gcgctatgaa cctgaggacg aaagcgcact
     2761 gtgcaaagcg atttggctcc tgtcgtgtca acaccatata cccgccagtc agcaaagaac
     2821 tggtagggtt cgcctacctc gatgaaccag agctcacctg atactttgga gcggaatacg
     2881 agtggcggcc tccacgtatc ggaaatgact ggttcaaatc ccgaattccc tcgggttttg
     2941 ttgatttctg caagaaacat tttcggttct attttgttct cagttatata gagggagtac
     3001 atatcacctc tccacccgac cgggttctca cgaacgacca aacgatatct gcctcctggt
     3061 ttcttttgcc aataaatact ccgggacgcc ttttcttcag aaattggtat cttatcaaat
     3121 tctgtatgat cgattttgat tgcgtctccc ccggagatgt caaggccatc tggccccagg
     3181 acaaggacac ttttcaagtc agagggaata actggaggag cataaagtac tgacgcatct
     3241 gattgaaata atgcatccgc tatccggagg gcttcgttgc actgaatgga cgtctttccg
     3301 ccaatcagtt taggccatga atcagcaaga acctgaggca aactgccagc ccatgcaatg
     3361 agaaccaaaa agaagataac ttttctcttc atgaccctct ccattttgtt agagtttttg
     3421 ttgtagcata acaaaaagct caacaggagc gaatcttttc cttgatctgt tgcagtgatg
     3481 ggtcatgctg ccctctccaa aaccatatca tctgtttgtg ttgttctatc cctaagattc
     3541 aatagtcttt cgtaatgtgc aatgaaaaaa tagcgcgggt cggatatgtc tgcatgaaat
     3601 gaagtcccgg gtgatagggg ttctttttga agattctata tgtttttctt gcttccttcc
     3661 ttgtctgatc aggaagttct ctataacatt tccagaaggt tgtgtggttt gagaggtcat
     3721 tgattcccta cgttcagaat cttacttttc cctgccttat gctctgcaag ggcttggtcg
     3781 gcaagtttat caaggatgtc ttcagacgaa gcaaaagtct cctgccattt tgcttcggct
     3841 tctaattcat ccatcaccca tttggcaaag gcattctgtt caacctcggg aagcttggat
     3901 gcctccttaa atgccttttc caatagtttt gccatggttc atatcgcttc taatctactt
     3961 cagagtattt taccataatg gtaccgtaaa atatcacctt caattctcaa ttttcatgta
     4021 taacgacctg cgcgtcagcc gcgctggtgc agtgtcgggt gcaacggctt gttatgcaat
     4081 aatttcattt aggccttcga taat
//
LOCUS       DAOOMC010000180         4097 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdus sp. isolate AMR_MDS_2685
            NODE_26313_length_4097_cov_7.812716, whole genome shotgun sequence.
ACCESSION   DAOOMC010000180 DAOOMC010000000
VERSION     DAOOMC010000180.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864760
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdus sp. (wastewater metagenome)
  ORGANISM  Syntrophorhabdus sp.
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae; Syntrophorhabdus.
REFERENCE   1  (bases 1 to 4097)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4097)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,729
            Genes (coding)                    :: 2,708
            CDSs (with protein)               :: 2,708
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 7 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4097
                     /organism="Syntrophorhabdus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26313_length_4097_cov_7.812716"
                     /isolate="AMR_MDS_2685"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2699756"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            47..2353
                     /gene="pglZ"
                     /locus_tag="PK125_12975"
     CDS             47..2353
                     /gene="pglZ"
                     /locus_tag="PK125_12975"
                     /inference="COORDINATES: protein motif:HMM:NF033450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BREX-1 system phosphatase PglZ type B"
                     /protein_id="HPB39057.1"
                     /translation="MNLKDKILEGFKKATVHNSSVMVAPEVILWPDLERQWEPVIGNL
                     QADYGALFVLGEYQPEKLTGPAIWLKTVVSGTLSDCGWHNDLTPIIYLPGISRSRLKD
                     VSSLGLDLQPLAEYQYTGTFFNQYNSKEWTVLALLTNQEDGLGLSVKQDSATKEALIK
                     SLPVYFEMDIAALSAPVIDAEFINGLVFPNVILSILKWMSIGDDFLSGMDPSLKETFR
                     DMCQSRYGFIPDHKNIKEIALKLGKQENAWKHVWSFYANSPSKYPIIETLLREAKPED
                     LGEGFFSIPCESWPQINEEREAQLRDDLEKLTKKDIEYIKSSITNLYDKHKERKKWVW
                     TELGYSPLVTALEYLTELLEYVNNPYPSASLNEIIQYYTQEGFYSDTCMRKAYAAVKT
                     EKDKSVIRELIQLIYKPWLERITEKFQNLVINNFSETKQQATKEEKHSFYLFVDALRY
                     EMAREYVSLLDKRGFKVGCTHLFAALPTLTSTAKVKSSPVSEEVNTTSEINDFTPFFK
                     TGKPANITNFRAELENKGYMHSIKKGFDPERSYWVEIGNIDTRGHEEQSEMLKRADEL
                     FDEISEVIDTAIEAGVREMKIVTDHGWLMLPGGLPSAKIVKDLTIARCGRCAYIKEGV
                     KTNLTHLPWEWNPSVYIGYAPDISFFRNNEEYAHGGISLQECIIPVLNIFIPQKEIIT
                     AKIVSHRWAGLRCIIETENTPDGFIIDIRTKYNDEKTSIVISSKQSISDNNCSVMVKD
                     SAIDSAAVIVLTDEKGKIIDKINTTIGN"
     gene            2357..>4097
                     /gene="brxL"
                     /locus_tag="PK125_12980"
     CDS             2357..>4097
                     /gene="brxL"
                     /locus_tag="PK125_12980"
                     /inference="COORDINATES: protein motif:HMM:NF024733.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0030163 - protein catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BREX system Lon protease-like protein BrxL"
                     /protein_id="HPB39058.1"
                     /translation="MELDNIDIIASKAFEGYIVRKDLLEQFRKAYPVPTYVIEFLLGR
                     YCATINPKEIEEGLDVVKRQLQDRTVRPGEEEFFKSKARERGSIRLIDIITARLDTGS
                     DSYVATLPSLRLNKVRISPDIVNANDRMLTGGFYAEIELEYDAAIAQENSGRPFGIAS
                     IRPIQLSKRDVLDLLYKGRESFTLEEWKDFLIRSVGLEPTSLTNKAKNVLFVRMVPFV
                     ERNYNLVELGPRGTGKSHLYQQISPYSHLVSGGKATVAKMFVNMGNGERGLVCKYDVV
                     CFDEVSGISFDQKDGVNIMKGYMESGEFSRGKEPIRADGGIAMVGNFDVDVEHQQRVS
                     HLFGPMPKEMRDDTAFMDRIHAFVPGWDFPKLNKTYLTEHFGMVSDFLAECWTRMRDV
                     SRLSSVLHRIDYGGALSGRDTSSVNKTLNGLLKLMQPDPEAPVSDELLEWAVKIALEY
                     RRRVKEQQKRVGSAEFRNTHFSYRIGEDGVETFVTTPEIHSENTIGEDPMPPGQIWTI
                     NMGGNHDEPTGLYKIEINAMPGSGVKILNKPAPPAFQECVRYAEQNLYSKAKELVGDR
                     DSRNHEFSVQLRAF"
BASE COUNT         1317 a          743 c          936 g         1101 t
ORIGIN      
        1 gaagagaaaa ggaaagtaag gcagaaataa taaattgcgt aattaaatga acttgaaaga
       61 taaaatactg gaaggtttta agaaagccac tgtacacaac agcagtgtga tggtggctcc
      121 cgaggttatc ctttggcctg atttggaacg gcagtgggaa cctgtaatag ggaatttaca
      181 agccgattat ggagctctgt ttgttctggg tgaataccag cctgaaaagc ttaccggacc
      241 tgccatatgg ctaaaaacag tggtttcagg gacgttgtca gattgtggtt ggcataatga
      301 tttgacgcct atcatttatc tgcccgggat ttcaagaagc cgtttgaaag atgttagcag
      361 ccttggactg gatctacaac cattggctga atatcaatat actggaacat tcttcaacca
      421 gtacaatagc aaagaatgga ccgtgttggc attacttacc aatcaggaag acggacttgg
      481 attgagtgtc aagcaggata gtgccactaa ggaagcattg atcaaatcat tgcctgttta
      541 ttttgaaatg gatatagctg cactctctgc tccggtaata gatgcggaat tcattaatgg
      601 gcttgttttc cccaatgtta ttctttcaat tttaaagtgg atgtccatag gtgatgactt
      661 tttatctgga atggatccat ccttaaaaga aaccttccgg gacatgtgtc aaagccgtta
      721 tggttttatc cctgatcata aaaatattaa agaaatagcc ctgaaactcg gtaaacagga
      781 gaatgcatgg aaacatgttt ggagctttta tgccaactca ccttcgaaat acccaataat
      841 tgaaaccctt ttaagagaag cgaaacccga ggatctggga gaagggttct tctcaatacc
      901 ttgtgaaagc tggcctcaaa tcaacgagga aagagaagca cagcttcgcg atgatcttga
      961 aaaactaaca aaaaaggata ttgaatatat aaaaagcagt ataaccaacc tttatgacaa
     1021 acacaaagaa cgaaaaaaat gggtatggac tgaactgggt tattctcctt tagtcactgc
     1081 attggaatat ttaaccgaat tattggaata tgtcaacaat ccatatcctt cggcgtccct
     1141 aaatgaaatc atacagtatt atacacaaga gggcttttat tcggatacct gtatgcgaaa
     1201 ggcttatgct gccgttaaaa ctgagaagga caaatcggtg atccgcgaat tgatacaact
     1261 gatatacaaa ccctggttgg aacgaattac tgaaaaattt caaaacctgg taattaacaa
     1321 cttttcagaa acaaaacaac aagctacaaa agaagaaaag cattcgtttt atcttttcgt
     1381 ggatgcctta cgctatgaaa tggccagaga gtatgtatca ttactggata aaagaggttt
     1441 taaggtcggg tgtactcact tatttgcggc acttcccact ttaacttcta ctgctaaagt
     1501 taaatccagc cctgtttccg aagaggtgaa cacaaccagc gagatcaatg attttactcc
     1561 ttttttcaaa acaggaaaac ctgccaacat tactaatttc agagcagaac ttgaaaataa
     1621 agggtatatg catagcataa aaaaaggttt tgatcctgaa agatcttatt gggtggaaat
     1681 cggcaatatt gacacacgcg gacatgagga acagtccgaa atgctcaaaa gagcagatga
     1741 gttatttgat gaaatttcag aagttattga tacagctatc gaagccggtg tcagagagat
     1801 gaaaattgtg acagatcacg gatggctaat gcttcccggg ggattgccat cggctaagat
     1861 cgtcaaagac ttgactattg cccgatgtgg aaggtgtgcc tacatcaaag aaggcgttaa
     1921 gacaaatttg actcaccttc cttgggagtg gaatccctcc gtttacattg gttatgctcc
     1981 ggatatttct ttttttagga acaatgaaga atatgcacat ggtggtatct ccttgcagga
     2041 atgtattatc ccggtattaa acatattcat tccacaaaaa gaaataataa ccgccaagat
     2101 tgtatctcat aggtgggctg gtttgcgatg catcatagaa acggaaaaca cgccggatgg
     2161 ttttataata gatattcgaa ccaagtataa tgatgaaaag acatccattg taatatcgtc
     2221 caagcaaagt atctctgaca ataactgcag tgtgatggtt aaagacagtg caatcgattc
     2281 agctgctgtt atagtcctca cagatgaaaa agggaaaatc attgataaga ttaatacgac
     2341 tataggaaat taagatatgg aacttgataa cattgatatt attgcgtcca aagcatttga
     2401 gggttatatt gttcgaaaag atcttctgga gcagttcagg aaagcttatc ctgtacctac
     2461 ttatgtcatt gaatttcttc taggaaggta ttgcgctacg atcaatccta aagaaattga
     2521 ggaaggcctt gatgtcgtta aacgtcagct gcaagataga actgtaagac ctggcgaaga
     2581 agaatttttt aagtcaaagg ccagggaaag aggatccata aggctgatag acatcataac
     2641 tgcccgttta gatacaggtt ctgatagtta tgttgccaca ttaccaagtt tacgattaaa
     2701 caaagtacgc atctctccgg atatagtgaa tgccaacgac aggatgctaa caggaggctt
     2761 ttatgccgaa atcgagttgg aatatgatgc tgccattgcc caagagaata gcggtcgccc
     2821 cttcggcatt gcctcaattc gtcccataca gttatcgaaa agagacgttc tagacttgct
     2881 ctataaaggc agagaaagtt ttacattaga agaatggaag gattttctga tccgaagcgt
     2941 tggccttgaa ccaacaagtt taactaataa ggctaaaaat gtgctttttg taaggatggt
     3001 accctttgtt gaacgaaatt acaacctggt agagctagga cccagaggca cgggtaaatc
     3061 tcacctgtat cagcaaattt ctccttactc acatttggta tcgggaggca aagccaccgt
     3121 agcaaagatg tttgtcaaca tgggtaatgg agaaaggggg ttggtttgca agtatgatgt
     3181 ggtctgtttt gatgaggtgt caggtatctc cttcgaccag aaagatggag taaatatcat
     3241 gaagggatat atggagagtg gagagttcag ccgcggaaaa gaacccattc gagcagacgg
     3301 aggaatagcc atggtaggca actttgatgt ggatgtagag catcagcaac gagttagtca
     3361 cctctttgga cctatgccca aagaaatgcg tgatgataca gccttcatgg atcgtattca
     3421 tgcgtttgtt ccgggatggg atttcccaaa attgaataag acatatttaa ctgagcattt
     3481 tgggatggtg agtgatttcc tggcagagtg ctggactcgg atgagagatg tcagcagatt
     3541 atcatctgtg ctacaccgta ttgattatgg cggggcttta agcggtcgtg atacctcttc
     3601 ggtgaataag acactaaatg gattgttgaa gttgatgcaa cctgatcccg aagcacctgt
     3661 ttctgacgaa ctcctggaat gggctgtaaa aatagctctt gaatatagaa gaagagtgaa
     3721 agaacagcag aaaagagtag gatctgcgga atttcgtaat actcatttta gctatcgtat
     3781 aggagaagat ggtgtggaaa catttgttac aacacctgaa attcacagtg aaaacactat
     3841 tggcgaagac cccatgcctc ccgggcagat ttggaccata aacatgggag gtaatcatga
     3901 cgaacccacc ggcttataca aaattgaaat caatgcgatg cccggctctg gtgtgaaaat
     3961 attaaacaaa cctgctcccc cggcatttca ggaatgtgtg cgttatgctg agcaaaacct
     4021 gtacagtaaa gctaaggaat tagtgggaga tagagattcc agaaatcacg agttctctgt
     4081 gcagctgaga gctttcg
//
LOCUS       DAOOMC010000181         4064 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdus sp. isolate AMR_MDS_2685
            NODE_26590_length_4064_cov_6.227488, whole genome shotgun sequence.
ACCESSION   DAOOMC010000181 DAOOMC010000000
VERSION     DAOOMC010000181.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864760
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdus sp. (wastewater metagenome)
  ORGANISM  Syntrophorhabdus sp.
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae; Syntrophorhabdus.
REFERENCE   1  (bases 1 to 4064)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4064)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,729
            Genes (coding)                    :: 2,708
            CDSs (with protein)               :: 2,708
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 7 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4064
                     /organism="Syntrophorhabdus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26590_length_4064_cov_6.227488"
                     /isolate="AMR_MDS_2685"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2699756"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..916)
                     /locus_tag="PK125_12985"
     CDS             complement(<1..916)
                     /locus_tag="PK125_12985"
                     /inference="COORDINATES: protein motif:HMM:NF005414.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016742 - hydroxymethyl-, formyl- and
                     related transferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyltransferase"
                     /protein_id="HPB39059.1"
                     /translation="MRAVVFAYHEIGYVCLEELIDFGIDVPCLFTHNDDPNEKIWFRR
                     PAELAHKKGIPIYTPDNLKEPSWAPIISGMKPDVIFSFYYRNLIPVEILSIPPIGAFN
                     LHGSMLPKYRGRCPVNWVLIAGETKTGLTLHYMVEKPDAGDIVVQKPVDISFDDNAYT
                     LFMKMAGTARILMKEILPHIKNRSFTRLSQDGMGQPSYFGGRKPEDGLISWQQDAISI
                     YNLTRAVTHPYPGAFTYLDGKKFFIWKAWPLNDLTDSLPGSVISTKPLAVSTGKGALT
                     LHRVQLDGGDEMDGEAFAEKYDVENKILG"
     gene            complement(913..1842)
                     /locus_tag="PK125_12990"
     CDS             complement(913..1842)
                     /locus_tag="PK125_12990"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HPB39060.1"
                     /translation="MDTKPYISVLIPLLNEQDSLPELQQRIRDALEGIGKPYEIIYIN
                     DGSIDNTQRILEDFHERFPGVKVIEFNRNYGQHMALFAGFQFCQGDIIITIDGDLQNP
                     PEEIPRLVSKMEEGYEVVATFRKNRHDSFFRRFASNIVNKITSRLVGVKLKDYGCMLR
                     AYSRPVIDHINMCTESSSFIPALANTFARKIVEIEVAHEERKKGQSKYSIFKLLRLNF
                     DLMTSFSLLPIQFIGVLGSVIALLGLFFALFLFIRRLIIGPESEGAFTLFGILFFFIG
                     IQIFALGVIGEYIGRIYQEVRRRPRYIIKKELF"
     gene            2320..2997
                     /locus_tag="PK125_12995"
     CDS             2320..2997
                     /locus_tag="PK125_12995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009240707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HPB39061.1"
                     /translation="MRILHVEDDKDIASFVIKGLKEAGHVVDHVENGEDGLHRALTEE
                     YDVAIIDLMLPKLDGLSLITEMRKKGKKTPVIILSAKRTVDDRVRGLEEGSDDYLTKP
                     FAFAELIARINALVRRASNIPDAAHLTVGDLSIDLPARRVVRAGQTIDLQPREFALLE
                     YLMRNAGRVVSKTMIMEHVWDYNFDPQTNVVETRVSRLRDKINRGFDRQLIETVRGAG
                     YVLRKDP"
     gene            3059..4054
                     /locus_tag="PK125_13000"
     CDS             3059..4054
                     /locus_tag="PK125_13000"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HPB39062.1"
                     /translation="MLVVVIAFSLLLLRGIHHVHLSPYTLHELLEAYLKFFGFSIACV
                     ALGSLFVGWFMARHALSGVIELTRTANAVAAGTLNERVPVKGRQDEIDYLAKTFNIML
                     EKINDLIQGMKETNDNIAHDLRSPITRMRGVAEAALSSSSSSEAHCLLAGHVVEQCDR
                     LLGMINTVLDISEAEAGVSKLDIEEIDVTEIVQDAVGLFLPMAEDKNITVQIQASSGV
                     KHYSDRRKLQRILGNLLDNAIKFTPSGGFITISVSSDTKKITIEVKDTGIGISEQDST
                     QIFDKFFRADRSRSEPGSGLGLSLARAFASSLGGSLTVKSSPGHGSVFTVTIRRT"
BASE COUNT         1100 a          881 c          946 g         1137 t
ORIGIN      
        1 ctccaaggat tttattttct acatcatatt tttccgcgaa ggcctcgcca tccatttcgt
       61 ccccgccatc cagttgcacc ctgtgaagtg taagtgcccc tttgccagta ctgacagcaa
      121 ggggtttggt tgaaataacg gaaccaggta agctgtccgt caggtcgttc aaaggccagg
      181 ctttccagat aaagaatttt ttgccatcaa gataggtgaa agcacctggg tagggatggg
      241 ttacagcacg ggttaaatta taaatcgaaa tagcgtcttg ctgccatgaa atgagaccat
      301 cttctggttt tcttccacca aaataggatg gttggcccat gccatcctgg gacagtctgg
      361 tgaatgacct attttttatg tgtggcagaa tctctttcat cagtatcctt gctgtaccgg
      421 ccattttcat gaataatgta taggcattgt catcaaatga tatatccaca ggtttctgga
      481 cgacaatatc tccggcatca ggtttttcca ccatataatg cagggtgagg cctgttttgg
      541 tttcacctgc aatcagcacc caattaaccg ggcatctccc cctgtacttt gggagcatgg
      601 atccatgaag gttgaaggcc cctataggcg gaattgaaag gatctccacg ggtatcaaat
      661 tcctgtagta aaaagaaaag atgacatccg gtttcatacc cgatatgatt ggcgcccatg
      721 atggttcttt cagattgtcc ggtgtgtaaa tcgggatgcc tttcttgtgg gcaagctctg
      781 ctggccgtct gaaccagatc ttttcattag ggtcatcatt gtgggtgaac aagcagggta
      841 catcgatgcc aaaatcaatc aactcttcga ggcagacgta tccaatctca tgataggcaa
      901 aaacgaccgc cctcaaaata gttccttttt aataatgtat cgtggtcttc tgcggacttc
      961 ctggtaaatt ctcccgatat actcgccaat aacaccaagg gcaaagattt gaatacctat
     1021 gaagaagaaa aggattccga agagagtaaa ggctccctct gactcaggtc ctataatcaa
     1081 ccttcggata aagaggaata gagcaaaaaa aagtccaagt aaggcaatga cgctaccaag
     1141 aacgccgatg aactgaatgg gaagaagaga aaaacttgtc ataagatcaa aattcaggcg
     1201 gagcaactta aagatgctgt attttgattg acccttcttt ctctcttcgt gggctacctc
     1261 tatctccact atctttcttg caaacgtatt tgcaagggca ggtataaaac tcgatgattc
     1321 agtacacatg ttaatgtggt cgatgaccgg gcgtgaatac gcacggagca tgcatccata
     1381 atctttcagc ttaaccccta cgagacgtga agtaatttta ttgacaatgt ttgaggcaaa
     1441 cctccggaag aatgaatcgt ggcggttttt ccggaacgtt gccacaacct catatccttc
     1501 ctccatcttg ctgacaagcc ttggtatttc ttcaggagga ttctgaaggt caccatctat
     1561 agtaatgata atatctccct ggcaaaattg aaaacctgcg aagagggcca tgtgctgtcc
     1621 gtagttcctg ttgaactcaa tcactttgac acctggaaat cgctcgtgga aatcttcgag
     1681 gatcctttgt gtgttgtcga tgctcccgtc atttatgtag ataatctcat atggtttgcc
     1741 aatgccttcc agtgcatcac gaatcctttg ttgaagctca gggagggaat cctgttcatt
     1801 gagaagggga atgagaaccg agatgtaggg tttggtatcc attcaaaagt ttttatatgc
     1861 taatatctga tatttttcaa taattatcta ttacagggtc tgtgtcggcc cctccatcta
     1921 aaccatttaa gcactttaag gggttcaggc tgttcgtact gtttgaacgg ctacagttgt
     1981 tcattctttt cgtccaagaa tggagtagtt gtttcaagcc atgccatgtg tcactctatg
     2041 ggccatgttc tctgccctct gtaaccatca ccggggcttc agaagacagg tcgtcctctc
     2101 aacggagagt tcctgtgtcc tccttgaagt ttattcaaaa tacatcatga aacctaacca
     2161 gaatattgcc aatttgttac caatttataa tattgcttct tgaggttatt ggcaaaaata
     2221 tattatcatt gggtatataa atgttttttc tcaaatctac tctgcgaact gtccatcttg
     2281 aacaagaaat gcatatatgt ttgatacaag gatattatta tgcgaatcct ccatgttgaa
     2341 gatgataaag atattgcatc cttcgtaata aagggtctca aagaagcagg tcatgttgtg
     2401 gaccacgtag agaacggtga agacgggctc catagggcgc tgactgagga gtatgatgtt
     2461 gccattatag acctgatgtt gccaaagctt gatggcctct ccctcataac cgagatgcgt
     2521 aaaaaaggaa aaaagacacc ggtgatcatt ctcagcgcca aacggactgt tgatgatcga
     2581 gttcggggcc tggaggaggg cagcgatgat taccttacaa aaccctttgc ttttgccgaa
     2641 ctcatagccc gtataaacgc tcttgtccga agagcgagca atatacctga tgctgcacac
     2701 cttactgttg gagacctttc aattgatttg ccggccagac gagttgtcag ggcagggcag
     2761 acgatcgact tgcaaccgcg ggagtttgct ttacttgaat acctcatgag gaatgccgga
     2821 cgggtagttt ccaagacgat gattatggaa cacgtatggg actataattt tgatccccag
     2881 acaaatgtgg tagagacaag agtaagcaga ttgcgcgaca aaatcaaccg tggtttcgac
     2941 agacagctta tcgagactgt gcggggagca gggtatgtcc ttagaaagga tccttaggct
     3001 acgtcataca gttgctttcc ggcttactgt gtggtacgcg gtgtttttca cagggggtat
     3061 gctggttgta gttatagcct ttagcctcct cctccttcgc ggcatccacc atgtacatct
     3121 ctccccttac acccttcatg aactgcttga ggcctacctg aaatttttcg gtttttccat
     3181 tgcctgtgtt gcccttggtt cactttttgt tggctggttc atggccaggc atgctctatc
     3241 cggagttatt gaacttacca gaactgcgaa cgctgtagct gcaggcacac tgaatgaacg
     3301 tgttccggta aaggggagac aggatgagat tgactacctt gccaaaacat tcaacattat
     3361 gcttgaaaaa atcaatgacc tgatccaggg gatgaaagaa accaatgata atatagccca
     3421 tgatctgaga agcccgatta cgaggatgag aggtgttgca gaggcggcat tatcgagcag
     3481 ctcctcatct gaggcccatt gtctgctggc tggacatgtt gtggaacaat gtgaccggct
     3541 ccttggcatg atcaacactg tacttgatat ttcggaggca gaggctggtg tatcgaaact
     3601 cgacatcgaa gagattgatg tcactgaaat tgttcaggat gctgtgggac tttttctgcc
     3661 catggctgaa gataagaata ttacggtaca gattcaggca tcgtccggtg taaaacatta
     3721 tagcgacagg cgtaaactcc agagaatcct gggaaatctt cttgacaatg ccataaaatt
     3781 tacaccatcg ggaggattca ttaccatatc cgtgagcagt gatacaaaaa agatcaccat
     3841 cgaagtgaag gatacgggaa taggaatatc tgaacaggat tctacccaga tttttgacaa
     3901 attctttcgg gcggacagga gcaggtcaga gccaggcagc ggccttggat taagtttggc
     3961 ccgtgctttt gcctcatctc ttggtggttc tttgacggta aaaagctctc caggtcatgg
     4021 gagcgtattt acggtaacca tacgtcgaac atgaaataag aatt
//
LOCUS       DAOOMC010000182         4000 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdus sp. isolate AMR_MDS_2685
            NODE_27169_length_4000_cov_5.676299, whole genome shotgun sequence.
ACCESSION   DAOOMC010000182 DAOOMC010000000
VERSION     DAOOMC010000182.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864760
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdus sp. (wastewater metagenome)
  ORGANISM  Syntrophorhabdus sp.
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae; Syntrophorhabdus.
REFERENCE   1  (bases 1 to 4000)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4000)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,729
            Genes (coding)                    :: 2,708
            CDSs (with protein)               :: 2,708
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 7 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4000
                     /organism="Syntrophorhabdus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27169_length_4000_cov_5.676299"
                     /isolate="AMR_MDS_2685"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2699756"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..582
                     /locus_tag="PK125_13005"
     CDS             <1..582
                     /locus_tag="PK125_13005"
                     /inference="COORDINATES: protein motif:HMM:NF013117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HPB39063.1"
                     /translation="DIGLRSTRLRTLSRTVVSIPNGQLATMSLENYNMRDKNLFRHNI
                     QLRYETSSDQLQFLIAGIRRLLYEHPKVETSGARVRFTGFKNSGLELEIFAYILEAEH
                     PVFLAIQEDLLLRFMNLVEQSGTSFSFPSQTTYIARDVGLNKEKTGKAVEKVDTWRKQ
                     GVLPFPDYKPDDIAEFENKLVYPESGSVFHKKA"
     gene            complement(632..1426)
                     /locus_tag="PK125_13010"
     CDS             complement(632..1426)
                     /locus_tag="PK125_13010"
                     /inference="COORDINATES: protein motif:HMM:NF015338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HPB39064.1"
                     /translation="MKQYLERLRVTTYNIHKCVGLDRLKRPERVVEVLREIDADIIGL
                     QEVVSIPGRNVEMDQARYIADALGMEYRMGTTRTMGDGVYGNTLLSRFPFMSNRTFDL
                     SRPGREERGCLCVDLAVAKDSILHIYNTHLGTAFFERRHQAKNIMRELISNLKHQNGA
                     RILLGDFNDWTRSLPTRLFSTHFSGEDILRRLERKRTYPGILPLFHLDHIYYDTGLVL
                     KNAILHKSTTALMASDHLPLVADFQLNFPKKADAGSGSNHHTVVYG"
     gene            complement(1386..2909)
                     /locus_tag="PK125_13015"
     CDS             complement(1386..2909)
                     /locus_tag="PK125_13015"
                     /inference="COORDINATES: protein motif:HMM:NF024491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HPB39065.1"
                     /translation="MKLSYKQMHTTRKPFVTEDWAEQVFLRTAGVPITQGNKVQILKD
                     AGENYPAWVESMKSAERWIHFESYIMHDDDAGREFSEILAAKAREGIRVKVIYDWLGG
                     LGKTSRRFWQRLRDAGVEVRCFNPPRFDSPLGWISRDHRKMIGVDGKIAFVTGLCVGS
                     MWAGNREKSIDPWRDTGISVTGPAVGDIEAAFAQIWVATGEPMPNDEIPEKESISQTG
                     NVALRVVASIPNVGGLYRLDQQIAALARHSLWLTDAYYVGISSYIQSLKAAAMDGVDV
                     RLLVPGATDIPVLRTISRAGYKPLLEAGVRVFEWNGSMIHAKTAVADGHWARVGSTNL
                     NVASWIGNYELDVVVENEDFAREMEDMYLADLENSTEIVLRKRWKIRSAAGKQGHKSA
                     SGPISGTGSVGRVGIGAIRISNTVGAAITNRRILGPAEIRITFVGSIALLGLAMLSIL
                     APRLVTVPIAIITGWFGIALLIKTWRLRRRRRREMEKNEGDKTYNETIPRTITGNDV"
     gene            complement(3141..3650)
                     /locus_tag="PK125_13020"
     CDS             complement(3141..3650)
                     /locus_tag="PK125_13020"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HPB39066.1"
                     /translation="MKDSVLKDKRILAVDDEPDVLEVLREEITMAVPTCVVDTATSYD
                     KAMELLASWTYDLAIFDIMGVRGFDLLRIAVNRDYPIPVVMLTAHALSPEFLKESIEK
                     GARAYLPKSSLGHVVPFLEDVLTYEYGDVWRRILKNVEGIFSSSRGPYWRKPDEDFWK
                     EFDEKIGKE"
BASE COUNT         1097 a         1019 c          787 g         1097 t
ORIGIN      
        1 gacataggcc tgcgttcaac ccgtcttcgc acgctcagcc gtacagtggt ttccattccc
       61 aatggtcagc ttgctacaat gagcctagaa aattataaca tgcgagacaa aaacctgttt
      121 cgtcataata ttcagttgcg ctacgaaacc tcttcagacc aactgcagtt tctcattgct
      181 ggaatacgga ggttacttta tgagcaccct aaagttgaaa catcaggggc tagagtacgg
      241 tttaccggtt tcaaaaactc tggtttagaa ttagagatat ttgcatatat tctggaggcc
      301 gagcatcccg tattcctcgc aatacaggaa gaccttcttc tccgttttat gaacctggtg
      361 gagcagagcg gcacatcctt ttcctttcca tcccagacaa catatattgc cagagatgtt
      421 gggcttaata aggaaaaaac cggaaaggca gttgaaaagg ttgatacatg gcgcaagcaa
      481 ggggtcctgc catttcctga ttataaacct gatgatattg cagaatttga aaataaactt
      541 gtatatcctg aatctggctc agtctttcac aagaaggcat gaaggcggca ataccaatat
      601 caatatcaac acaccgtgtt tctgtgacct ttcaaccata tacaacagta tggtgattgg
      661 accctgaccc tgcatctgct ttttttggaa aattgagctg aaaatccgca accagcggaa
      721 gatggtctga cgccataagg gctgttgtgc ttttgtgaag tatggcattt tttagaacta
      781 accctgtatc ataatagata tgatccagat gaaacagggg caatataccc ggataagtcc
      841 tcttgcgctc cagcctccgg aggatgtctt ccccggagaa atgggtcgag aaaagtctgg
      901 tcggaaggct tctggtccaa tcgttaaaat cgcccagaag aatgcgggca ccattctggt
      961 gtttaagatt tgaaattaat tctctcatta tatttttagc ttgatgtcgt cgttcaaaga
     1021 aggcagtgcc aagatgggta ttatatatgt gcagtatact gtcttttgcc actgccaaat
     1081 caacgcagag gcatccacgt tcctcccgac ccgggcggct caggtcaaag gtacgattcg
     1141 acatgaaagg aaagcggcta agaagtgtgt ttccatagac cccgtccccc attgtccggg
     1201 tggttcccat cctatattcc atgccaaggg catctgcaat atatcgggcc tgatccatct
     1261 caacattcct tcccggaatg ctaaccacct cctggaggcc aatgatgtca gcatctattt
     1321 ctcgcaaaac ttccacgacc cgttccgggc gcttgagacg gtcgaggccc acacatttat
     1381 gaatgttata cgtcgttacc cgtaatcgtt cgaggtattg tttcattgta cgttttatct
     1441 ccttcgttct tttccatctc cctgcgtctc cttcggcgca gacgccatgt cttaattaac
     1501 aaggcaatcc caaaccaccc agtgataata gcgatcggaa cagtaaccag tctcggggca
     1561 agaatagata gcatggcaag ccccaaaagt gcaatactcc ccacaaaagt gatgcgtatc
     1621 tcggcaggcc caagaatgcg gcggttggtt atagcagcac caactgtatt gctgatgcgg
     1681 attgctccaa tccccaccct tcccacgctt cctgtaccag aaattggccc ggaagctgat
     1741 ttgtgccctt gtttccctgc agcagacctt atcttccatc gctttcttaa tactatctct
     1801 gttgagtttt ctaggtctgc aagatacata tcttccattt cccgagcaaa atcctcattc
     1861 tctacaacca catcgagttc atagttacca atccagcttg caacgttcag gtttgtagat
     1921 cccacacgag cccaatgtcc atctgccaca gctgttttcg catgaatcat ggagccattc
     1981 cattcaaata ccctcacccc tgcttcaagg agcggcttat atcctgccct tgatatggtg
     2041 cgtaagaccg gtatgtcagt agctcccggg acaaggagcc gaacgtcaac cccatccatc
     2101 gcagcagcct taagagactg gatataagac gaaataccca cgtagtatgc atctgttagc
     2161 caaagggaat gcctggccag ggctgctatc tgttgatcaa gcctgtatag gccacccaca
     2221 ttcggtatac ttgctaccac acgcaaagcc acattccctg tctgactaat cgattctttt
     2281 tcgggaattt cgtcattagg cattggttct ccggtagcaa cccatatttg ggcaaaggct
     2341 gcctcaatat ctcctaccgc tggacccgtg acactgatac cggtatcacg ccatggatca
     2401 attgactttt cacggttacc ggcccacatg ctacccacgc aaagtccggt aacaaaagct
     2461 atttttccgt caacacctat cattttccgg tgatccctgc tgatccatcc cagaggactg
     2521 tcgaatctcg gtggattgaa acaacgaacc tcaacaccag catccctcag cctctgccaa
     2581 aaccttcggg aagtcttacc caaacctccg agccagtcat aaatgacttt tactctgata
     2641 ccctcccgcg cttttgccgc aagtatctcg ctgaattccc tcccagcatc atcgtcgtgc
     2701 atgatgtaac tttcgaaatg aatccacctt tctgccgatt tcatagactc gacccaggcc
     2761 ggatagtttt ctcctgcgtc cttgaggatc tggactttat ttccttgagt tatcggcaca
     2821 ccagcagtcc tcaaaaagac ttgttcggcc cagtcttctg taacaaaggg ctttctggtg
     2881 gtgtgcatct gcttatatga taatttcatc gggctattat atcacagcta tcgtgatatt
     2941 cttcattcga atccaagaat gtgtattaca gtatgattgt ttgatcatat gctgaccgaa
     3001 ctgtacaaaa cctattttcg cttttgttga agtttttctt aacaggcttc actgaactta
     3061 tcgtcaaaaa ccatgggaga caatacaaat aaaatttaca aattctatgg agttgcagta
     3121 ttcgtctcag acaacccccg ttattcctta ccaatctttt cgtcaaactc tttccagaag
     3181 tcctcatcgg gcttacgcca atatggacct cgcgaactac taaagattcc ttccacattc
     3241 ttgaggatgc gtctccatac atcaccatat tcatatgtca gaacatcttc cagaaaagga
     3301 acaacatgcc ccagactcga tttagggaga tacgctctgg cacccttctc tattgattcc
     3361 ttcaaaaatt caggagacaa tgcatgggcc gtaagcataa ccactgggat ggggtagtcc
     3421 cggttcacag cgatccggag cagatcaaaa ccccgaaccc ccatgatatc aaaaatggca
     3481 aggtcatagg tccatgaagc caaaagctcc attgctttgt cataggaggt ggcagtgtcc
     3541 acaacacagg ttgggacagc cattgtaatc tcttccctta atacttccag cacatccggt
     3601 tcatcatcca cagccaaaat cctcttatct ttcaaaacag aatccttcac aggaacctcc
     3661 tttcaaaaaa ataatatatc ataacaactt ttaatataaa atccatcttt cctcattggt
     3721 tcacaaatgt tcgttaggca tctgatgtga tgtctgtcaa taatataacc ggcgatttgt
     3781 acgataatag gaaagtgaca agggcaaaaa aaatcacatt aaaaccatct taccacggag
     3841 attgaagcaa tgcgaaacga gaaaaaagtg agagaaataa tccacatatt aataggaagc
     3901 aggttttatt tttcctttag tgttcaagaa agatatgatt tcatcaggaa cataatcagg
     3961 cgatttcctt tttcctcata atattataca cttactatta
//
LOCUS       DAOOMC010000183         3940 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdus sp. isolate AMR_MDS_2685
            NODE_27745_length_3940_cov_4.239382, whole genome shotgun sequence.
ACCESSION   DAOOMC010000183 DAOOMC010000000
VERSION     DAOOMC010000183.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864760
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdus sp. (wastewater metagenome)
  ORGANISM  Syntrophorhabdus sp.
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae; Syntrophorhabdus.
REFERENCE   1  (bases 1 to 3940)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3940)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,729
            Genes (coding)                    :: 2,708
            CDSs (with protein)               :: 2,708
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 7 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3940
                     /organism="Syntrophorhabdus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27745_length_3940_cov_4.239382"
                     /isolate="AMR_MDS_2685"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2699756"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..797
                     /locus_tag="PK125_13025"
     CDS             <1..797
                     /locus_tag="PK125_13025"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HPB39067.1"
                     /translation="LVLVLIMLAGIIVITVYKGAPALTFSMLVEIPKGGYYLGKEGGI
                     ANAIVGSLLLALGASFCSILIAIPVAFGLQKEYTSWRYAGFTKLVLDVLWGTPSIVYG
                     AFGFVVMVYFGLRASLLGGIIVLTLLMLPIMVRSMEEIIRTIPLELKEISYAMGTTKI
                     ETTLAVVLRQALPGIVTAVLLAFGRGIGDAASILFTAGYTDFIPRSIFDPVASLPLAV
                     FFQIGTPIPEVQERAYASALVLLLIVLCVSLISRILSHRFSKNIVR"
     gene            808..1623
                     /locus_tag="PK125_13030"
     CDS             808..1623
                     /locus_tag="PK125_13030"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HPB39068.1"
                     /translation="MPHISLKNLSIAYGHHRVLQDVTVNIPDKQITVIIGPSGCGKTT
                     LLKSLNRLLDLNEDVKVTGDVLIDGVNTYDGNADILALRKKMGFLSQRPYPLPMSIYE
                     NIAFGPKVHRMSKDRIVQQINDLEGRMVQVPSKKNGQMDILVESYLRLAGLWEEVKDR
                     LHTPASRLSIGQQQRLALARALAVEPEAILADEPTSALDPLSAQLIEKQFKFLKKNYT
                     IVVVTHILRQARRIADYVIFLYLGELVEHGPAEKVFDNPSDERTRAYVSGEIS"
     gene            1752..3026
                     /locus_tag="PK125_13035"
     CDS             1752..3026
                     /locus_tag="PK125_13035"
                     /inference="COORDINATES: protein motif:HMM:NF013799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HPB39069.1"
                     /translation="MQKKALIVGIDGVPFGLITRMMEQGHMKELKHMCEGDYTIHKMH
                     ASYPDISSVSWTSFMTGANPAEHGIYGFTDLKPNSYDLYFPNSRDIKAPTFWQTLQKQ
                     KKIKRVLVLNIPNTYPAFPIDGLLVSGFVAIDFNKAVYPTSYVAPLQRMGYVIDVDLM
                     KARQDKDGFYQDLMDSLAVREKVSINLITEEEWDIATICITETDRLHHFFFDKKDTPV
                     FHDFYKRVDTFIGNIYRAFEKKYGKDFLFLVLSDHGFTEQVAEVNLNALLRDSGYLVT
                     DESRDYYDKIEKGTIAFAMDPGRIYIHYEKKFPRGQVKSSETEQIKKALKAVFLELAD
                     TNGTKVIKEVVDKEEIYKGPYYDNAPDLICISNEGYDLKGNLRRKEVFSSDIFKGMHT
                     GEDATLITPKNITFDQQVTIENPPNIIMNYFL"
     gene            3046..3849
                     /gene="cysD"
                     /locus_tag="PK125_13040"
     CDS             3046..3849
                     /gene="cysD"
                     /locus_tag="PK125_13040"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: protein motif:HMM:NF003587.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009336 - sulfate adenylyltransferase
                     complex (ATP) [Evidence IEA];
                     GO_function: GO:0004781 - sulfate adenylyltransferase
                     (ATP) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0019419 - sulfate reduction [Evidence IEA];
                     GO_process: GO:0070814 - hydrogen sulfide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HPB39070.1"
                     /translation="MDHLDALENKSIYIIREAYYRFKNIAMLWSVGKDSTTLLWLIRK
                     AFFGKIPFPIIHIDTSYKFPDMYEFRDRLAREWNFPLIVGRNEEKLAAGMSPSSGMKL
                     ECCTELKTNALKQVIDKYKFKAIFLGIRRDEHGIRAKERYFSPRDDQFQWNYENQPAE
                     IWDQYKSYLDKEEHYRVHPILHFTELDIWKYIQREKLPIIHLYLAKEGKRYRSIGCVP
                     CCSPIESSATTIEDMINEVAASKTSERAGRAQDKENIYTMQKLRALGYM"
BASE COUNT         1172 a          861 c          873 g         1034 t
ORIGIN      
        1 ctcttgttct tgttctcata atgcttgccg ggatcattgt cattactgtg tacaagggtg
       61 caccagccct caccttctca atgcttgtag agataccgaa aggtggttat tacctgggta
      121 aagaaggtgg catagccaat gcgattgttg gatctctgct ccttgcactc ggtgcatcgt
      181 tctgctctat cctcatagcc atccccgtgg cctttggcct tcaaaaagaa tacaccagtt
      241 ggcgttatgc cggattcacg aaacttgttc ttgatgtgct gtggggcaca ccatccatcg
      301 tatatggtgc ttttggattt gtcgtcatgg tttacttcgg actccgtgcc tcccttctcg
      361 gaggtattat cgtgctgacc ctcctcatgc ttcccattat ggtacgatca atggaggaga
      421 ttatacggac cattcctctt gaattaaagg aaatttcata cgccatgggc accactaaga
      481 tagaaacaac actggcagta gttctgcggc aggccttgcc aggcatcgta acagctgtcc
      541 tccttgcttt tggacgaggt attggggatg cggcatcaat ccttttcacg gcaggttaca
      601 ctgactttat cccccgatcg attttcgatc ctgttgcttc ccttcccctc gcagtctttt
      661 ttcaaatagg gacacctata ccggaagtcc aggaacgggc gtatgcttca gcgcttgtgt
      721 tacttctcat tgtactttgt gtaagtttga tatcccgcat cttgagccac agattttcaa
      781 aaaatattgt gaggtagata acaacctatg ccccatatta gcttaaaaaa tctatccatc
      841 gcatatggtc accacagggt gctccaggac gttacggtta atatccctga taagcagata
      901 acagtcatta tcggtccctc tggatgtggg aaaactaccc ttttgaaaag tctgaatcgc
      961 ctcctcgacc ttaacgaaga tgtaaaggtg accggggatg tacttataga cggtgtaaac
     1021 acatatgatg gtaatgccga catccttgct cttagaaaga agatgggctt cctctctcaa
     1081 agaccttacc cccttcctat gtctatttat gaaaacattg cttttggccc aaaggtacac
     1141 cggatgtcaa aagacagaat agtccagcaa attaacgatc ttgaaggtcg tatggtccaa
     1201 gtgccttcga aaaaaaatgg ccagatggat attcttgttg aatcatattt gcgacttgcg
     1261 ggactctggg aagaggtaaa agacagactc catactcctg catcgaggct ctctataggg
     1321 cagcaacaga ggcttgccct tgcccgtgca ctggctgtgg aaccggaagc catcctagcc
     1381 gatgagccta cgtcagccct cgatcccctg tcagcgcagc taattgaaaa acagtttaaa
     1441 ttcctcaaga aaaattacac tatagtggta gtgacccata tattacgtca ggctcggaga
     1501 attgctgact atgtgatctt cctctatctt ggagagcttg ttgagcacgg gcctgctgag
     1561 aaagtttttg acaacccttc agatgaaagg acgcgggcat acgtgtcagg ggaaataagt
     1621 taatgagata agcaaattgc aaggaataat aggcgataga gagatggaaa agatacaatg
     1681 cacggaagat gaggagttta acgaaaaaaa gttgaaaaac aagggcaaag ttcactatac
     1741 aggaaaataa catgcagaag aaagcactta tagttggtat tgacggtgtt cctttcggct
     1801 taatcaccag aatgatggag cagggacaca tgaaagaatt gaagcatatg tgcgaaggag
     1861 attacacaat tcacaagatg cacgcatcct atcctgacat ttccagcgtg tcgtggacat
     1921 cattcatgac aggcgctaat cctgctgagc acggtattta tggctttact gaccttaagc
     1981 caaattctta tgacctttac ttccccaact ctcgtgacat taaagcaccc accttctggc
     2041 agaccctcca gaaacaaaaa aaaataaaac gggttcttgt tctgaacatc ccgaatacct
     2101 accctgcctt tcccatcgat ggtcttctcg tatctggatt cgttgctatc gattttaaca
     2161 aagcagtcta ccctacctct tatgttgctc ctttacagcg tatgggatac gttattgatg
     2221 tggacctcat gaaggcaagg caggataagg atgggttcta ccaagatctc atggatagcc
     2281 tggctgtaag agaaaaggtg tccatcaacc ttatcactga ggaagagtgg gacatagcta
     2341 caatctgcat aacagagact gacaggcttc accatttttt cttcgataaa aaagatactc
     2401 ctgtatttca cgatttctac aaaagagttg acaccttcat tggtaatatt tacagggcat
     2461 ttgaaaagaa atatggcaaa gattttctct ttctcgtcct ctctgatcat ggtttcaccg
     2521 aacaggttgc agaagtgaat cttaacgccc tgctcaggga cagtggatat ctcgtaacag
     2581 atgaaagtag agattactat gataaaattg aaaaggggac tatagctttt gccatggatc
     2641 cggggcgtat atatatccat tacgaaaaga aattcccccg tggccaggta aaatcctctg
     2701 aaacagaaca gattaaaaaa gcgctgaaag cggttttcct agaactcgct gacactaatg
     2761 gcacaaaagt catcaaggaa gttgtcgata aggaagagat ttacaagggg ccttactatg
     2821 ataacgcacc tgacctgata tgcatttcaa atgaaggata cgacctcaag gggaatttga
     2881 ggagaaagga ggttttctca tcagacattt tcaaaggtat gcacacaggt gaggacgcaa
     2941 cccttataac accaaaaaac ataacctttg atcaacaggt gactatagaa aaccccccta
     3001 acataattat gaactatttt ttatgataca ggaaggaggt cttggatgga tcatttagac
     3061 gcgctcgaaa acaaaagtat ctacatcatc agagaagcgt actaccgttt taagaatatt
     3121 gccatgctat ggtctgtagg caaggattct actacattgt tatggctcat acgaaaggca
     3181 ttttttggca agattccttt ccccataatc cacatcgaca cgagctacaa gtttcctgat
     3241 atgtatgagt tcagagacag gcttgctcgt gaatggaatt ttccccttat tgtaggaagg
     3301 aatgaggaaa aacttgcagc gggcatgagc ccatcttctg gcatgaagct ggagtgctgc
     3361 acagaactta agacaaatgc cttaaagcag gttattgaca aatacaagtt taaagcaata
     3421 tttctgggta tcaggcggga tgagcacggt atcagagcta aggaacggta tttctctccc
     3481 agggatgacc agttccagtg gaattatgag aatcagcctg cagagatatg ggaccaatat
     3541 aaatcatacc tggacaaaga ggagcattac cgggttcatc cgattcttca tttcacagaa
     3601 ctcgatatct ggaaatatat ccagcgtgag aaactgccaa tcattcatct ctacctggca
     3661 aaagaaggaa aaagatatcg cagcatcgga tgcgtaccct gttgttctcc tattgaatca
     3721 agtgccacaa ccatcgaaga tatgattaat gaggtggcag catcaaaaac atcggaacgg
     3781 gctggaaggg cacaggacaa ggaaaacata tataccatgc aaaaactgcg ggcattagga
     3841 tacatgtagg aggaaggaaa tggaagaaaa agcattaccc atcgtaataa caggccacat
     3901 tgaccacgga aagagcaccc tcattgggag actcctctat
//
LOCUS       DAOOMC010000184         3937 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdus sp. isolate AMR_MDS_2685
            NODE_27776_length_3937_cov_7.884596, whole genome shotgun sequence.
ACCESSION   DAOOMC010000184 DAOOMC010000000
VERSION     DAOOMC010000184.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864760
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdus sp. (wastewater metagenome)
  ORGANISM  Syntrophorhabdus sp.
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae; Syntrophorhabdus.
REFERENCE   1  (bases 1 to 3937)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3937)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,729
            Genes (coding)                    :: 2,708
            CDSs (with protein)               :: 2,708
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 7 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3937
                     /organism="Syntrophorhabdus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27776_length_3937_cov_7.884596"
                     /isolate="AMR_MDS_2685"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2699756"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            245..1888
                     /gene="uvrC"
                     /locus_tag="PK125_13045"
     CDS             245..1888
                     /gene="uvrC"
                     /locus_tag="PK125_13045"
                     /inference="COORDINATES: protein motif:HMM:TIGR00194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HPB39071.1"
                     /translation="MLDEHILDNLPEASGVYFFKDDDGKIIYIGKARNLRDRVKSYFR
                     EGPRDPKTEHLIKNIKHVDVLLTENEKEAFLLENNLIKEHTPKYNVNLRDDKTYISLK
                     VSLQHTFPGIYITREIKNDGALYLGPYPHARDVKNVLKLVQGIYPVRRCRDSVFKKRK
                     RPCILYDIGKCRAPCSGKVDQTQYRRIVDEMVDFLSGKNEKILKDIKKQIEEAGAAWK
                     FDEAKSLKERYLTIQEMIEKQNVHEHFGKNRDVWAFTSSENGLKSVLLCFRRGLLIAK
                     RKFHDKTAAMPMNEAISSFIFQYYDTRALPDEIILTEELDDFPVFEKHFKETKQKTVK
                     LYGPRSKVGREMVRLAIENLHEPEPVLLDEAFKRALHMTKNPIRIEAYDISHTHGKNP
                     TGVMVVFQGFTMAKNAYRVFHIRGESTMDDVAMMGEVLRRRLTDKKIVPFPDLIVIDG
                     GKGHLSAGVKILRDLDIAIDIISIAKDQRRKNMEDTIYLPNRKNPLPLAKSSSVMKEI
                     VKMRDEAHRFAISSHKRWKSRDDLAKGNSSQNRKPGLEN"
     gene            complement(2067..2936)
                     /locus_tag="PK125_13050"
     CDS             complement(2067..2936)
                     /locus_tag="PK125_13050"
                     /inference="COORDINATES: protein motif:HMM:NF013077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HPB39072.1"
                     /translation="MPIVTIDPQRCKKDGLCVRICEKVFEQKLEGSVPVVTHEEHCNS
                     CGHCVLICPAGAITQKECPDEKVHPVNREILPSYESLREMIASRRSIRNFQKRDVETE
                     VIEKVIGGACFAPSAKNTQSTQFLVIQEHNLLRGIASATAEWLGKTSRQLKNPFLRKL
                     YLLRGEKSAEEVTRWISQFELIVERMRRGSDPIIRNAPVLLLFHADQSVAFAEANANL
                     AVQNAMLIASSLGLGTLYTGYVVAASGRDKTIQRLLKLPKKHKVYGGLALGYPEITFS
                     KWIDRNPAVITWR"
     gene            complement(2988..3344)
                     /locus_tag="PK125_13055"
     CDS             complement(2988..3344)
                     /locus_tag="PK125_13055"
                     /inference="COORDINATES: protein motif:HMM:NF018753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DRTGG domain-containing protein"
                     /protein_id="HPB39073.1"
                     /translation="MTLREMIDLLDAEVFVGHDKLDLDITKAGCSDSMSDVQMIGQAG
                     MLLLTGQTNPHAIQTAHNVGIAAVIIVRGKRPLSETVQLAKELQMPLLSTKYILFETA
                     GRLYSKGVVGNVQKIE"
     gene            complement(3540..>3937)
                     /locus_tag="PK125_13060"
     CDS             complement(3540..>3937)
                     /locus_tag="PK125_13060"
                     /inference="COORDINATES: protein motif:HMM:NF021127.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HPB39074.1"
                     /translation="EENRGNVIRFARSVVDAGADMVIGHGPHVLRAMEVYRGRLIAYS
                     LGNFLTYALFNLKKPNCLSVILKARIDTGTGNFLDGRLVPLRLVNGGIPEPDTSGESI
                     RLIKELTAADIKPWSIVIEDNGIIRPLRK"
BASE COUNT         1166 a          890 c          852 g         1029 t
ORIGIN      
        1 ctgtcaaaga tggtaaacaa actgcgaaag gagataaacg aggcagccag gcaatgggat
       61 ttcgaaaggg cagcccaatt acgagaccga ctcatgaagc tcgaaaagat ggagattgca
      121 ttatgacaaa gggaaaaatg ctgtaaaaca ttatgagtaa cgcgtaacgg gttcacgaca
      181 catctcactg attacgcgtc acgcgtcacg gatgttatcc tattgcctat agcctgactt
      241 tattatgctt gatgaacata tcctcgacaa tcttcccgaa gcctccggtg tctatttctt
      301 taaagatgat gatggcaaaa tcatatacat tggtaaagca agaaacctcc gtgacagggt
      361 aaaaagctat ttcagagaag gtcccaggga tccaaaaaca gaacatctaa tcaaaaacat
      421 taagcacgtc gatgtcctct taacagagaa cgaaaaagag gcctttctcc tggaaaataa
      481 tctcatcaaa gaacatactc ctaagtataa cgttaacctt agggacgaca aaacatacat
      541 ttcccttaaa gtaagcctgc agcatacatt tccaggcatc tatatcacga gggagattaa
      601 gaacgacggc gccctttatc ttgggccata cccccatgcc cgtgatgtga agaacgttct
      661 caagctggtt cagggtattt atcctgtcag gcgatgcaga gattcggtgt tcaagaaacg
      721 gaaacgaccc tgcattctct atgatatagg caagtgcagg gcaccgtgct caggaaaggt
      781 tgaccagact caatatcggc ggattgttga cgaaatggtg gattttctct caggtaaaaa
      841 tgaaaaaatc ctcaaggata taaaaaaaca gatagaggag gctggagctg cctggaaatt
      901 tgatgaagca aaatctttaa aggaaaggta tcttaccatt caagaaatga ttgagaaaca
      961 gaatgttcat gagcactttg gaaaaaacag ggatgtatgg gcctttactt ccagtgaaaa
     1021 cgggctgaaa tctgttcttc tgtgctttag gaggggtctc cttatcgcta aaagaaagtt
     1081 tcatgataaa accgctgcca tgcccatgaa cgaagcaata agctctttta tcttccaata
     1141 ttatgatacc cgcgcactac ccgacgaaat aattctcaca gaagaactgg atgatttccc
     1201 tgttttcgaa aaacatttta aagagacaaa gcaaaaaact gtgaaactgt atgggccacg
     1261 ctcaaaagta ggcagggaga tggttaggct tgctatagaa aacctccatg agccagaacc
     1321 agtgctgctg gatgaagcat tcaaacgtgc cctccatatg acaaagaatc ccattcgaat
     1381 tgaggcttat gacatttccc acacacacgg gaagaatcct acaggtgtaa tggtcgtttt
     1441 ccagggtttc actatggcaa agaatgccta ccgggttttt cacatcaggg gagaatcaac
     1501 catggatgac gtagccatga tgggtgaagt gctccgacga cgcctgaccg ataaaaagat
     1561 tgtcccattc cctgacctca ttgtgattga cggtggaaaa ggccaccttt cagccggcgt
     1621 aaaaatatta agagatctcg atattgccat cgatataatc agcatagcaa aggatcaacg
     1681 cagaaaaaac atggaagaca cgatctattt gcctaaccga aaaaaccctc ttccactggc
     1741 aaaatcttct tctgtgatga aggaaattgt gaagatgagg gacgaagccc atcgattcgc
     1801 catttcatcc cacaaaaggt ggaaaagtcg ggatgatttg gcaaaaggaa atagttcaca
     1861 aaacagaaaa ccgggattag agaattagtg ttacaaaaac gactttttta ttccagataa
     1921 acgctggttt tgtgcagatc acttcggttt tgagttagaa aatattatgt aactgagaac
     1981 ttatacctca taactgcctc atttcacacc cgctcacagt aaatacacag cctgatacgc
     2041 tggcacgcga tattagtagc gcctttctac ctccaggtta ttaccgctgg attgcgatca
     2101 atccattttg aaaatgtaat ttctgggtat cccagagcca accccccata taccttatgt
     2161 tttttaggaa gtttaagaag gcgttggatt gtcttatctc ggccggatgc tgcaacaaca
     2221 tatcctgtat acaaagtgcc cagaccgaga gatgaggcta tcagcatcgc attctgaaca
     2281 gcgagattgg cgtttgcttc tgcaaaagct accgactgat ctgcatgaaa aagcagaaga
     2341 accggtgcgt tgcgtataat cggatcagaa ccacgtctca tcctttccac gatgagttcg
     2401 aactgactga tccatctggt aacctcttct gcgctctttt cgccacgcaa aagatagagt
     2461 tttctcaaga aagggttctt gagttgtctc gaagtttttc caagccattc agctgtggca
     2521 gatgcaatgc ctcggagcag gttgtgttcc tggatcacta aaaattgtgt gctctgcgtg
     2581 ttcttggcac ttggagcaaa acaggcgcca cctataactt tttctataac ttccgtctcc
     2641 acatctctct tttggaaatt ccttatggaa cgtctcgaag caatcatttc tcggagagat
     2701 tcataagaag gaaggatttc ccgatttacc gggtgcactt tttcgtccgg acactccttc
     2761 tgtgtgatcg cacctgcagg acatataagt acgcagtggc cgcacgagtt gcagtgttct
     2821 tcatgagtta caaccggcac agagccttcg agcttctgct cgaaaacctt ctcacagatc
     2881 cgaacacata aaccatcttt tttacagcgc tggggatcga tggttacaat aggcatgggc
     2941 gctttatgtc cgagagatac attattagga gcctggcttt atggtattca ttcgatcttt
     3001 tgcacattac cgacaactcc tttcgagtac agcctgcccg ctgtttcaaa gaggatgtat
     3061 ttggttgaaa gaaggggcat ctgtaattcc tttgcgagct gaactgtttc cgaaagaggt
     3121 cgttttcccc gcacaatgat aacagccgct attcccacat tatgggcagt ctgaatggcg
     3181 tgtggatttg tctgtccggt caacaacaac atcccggctt gaccaatcat ttgcacatca
     3241 gacattgaat cggaacaacc tgcctttgtt atatccaggt ccagcttgtc atggccaaca
     3301 aagacctcag catcaagcag gtcgatcatt tctcgtaatg tcaccgtaag ctccttgcat
     3361 ggagtgaaat agactataaa tataacaaaa cagagatgaa atgagccaat aaatttatgc
     3421 caggaaagat ttataaaaac tgtccagttt tcccgtgata ccatacgatt gtactatcat
     3481 gtatgcagtt gcgggcgtaa atgttgtcct gaccattgac gattaaaaac aatcttccat
     3541 cattttcgca aaggacggat gataccgttg tcctctatta caattgacca tggtttgatg
     3601 tctgctgcag tgagttcttt gatgagtctg atcgattcgc cgctggtgtc cggttcaggg
     3661 attccgccat tgaccaggcg cagcggtaca agccttccat cgagaaaatt gcctgttcct
     3721 gtatcaatcc gcgccttcag aatgacactc aaacagttcg gtttcttcaa gttgaataag
     3781 gcgtaggtga gaaaatttcc caggctgtag gcaattagcc tgcccctata gacttccata
     3841 gccctgagca catgaggacc atggcctatc accatgtctg ctccagcatc gacaacactc
     3901 ctggcaaatc tgatcacatt ccctctattt tcttcca
//
LOCUS       DAOOMC010000185         3914 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdus sp. isolate AMR_MDS_2685
            NODE_28006_length_3914_cov_5.100026, whole genome shotgun sequence.
ACCESSION   DAOOMC010000185 DAOOMC010000000
VERSION     DAOOMC010000185.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864760
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdus sp. (wastewater metagenome)
  ORGANISM  Syntrophorhabdus sp.
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae; Syntrophorhabdus.
REFERENCE   1  (bases 1 to 3914)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3914)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,729
            Genes (coding)                    :: 2,708
            CDSs (with protein)               :: 2,708
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 7 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3914
                     /organism="Syntrophorhabdus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28006_length_3914_cov_5.100026"
                     /isolate="AMR_MDS_2685"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2699756"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            368..1234
                     /gene="sppA"
                     /locus_tag="PK125_13065"
     CDS             368..1234
                     /gene="sppA"
                     /locus_tag="PK125_13065"
                     /inference="COORDINATES: protein motif:HMM:TIGR00706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HPB39075.1"
                     /translation="MTRIKKAFLVIFILIAVIFVVSFLTGIFGRGLGDKIGVVEIEGV
                     ITDGKDTMEDIVKFKEDDGIKGVIVRINSPGGSVGPSQEIYRELKKLSVKKKVYISAS
                     SVCASGGYYIASAGEKIFANPSTITGSIGVIMEQVVAEDLMKKIGIQPNTIKAGQFKD
                     VGSPFRKMKDDERAYFQQILDSIHEQFIKDVAEGRKMSVEQAKKLSDGRVYTGSQAKE
                     LGLVDSIGTFYDTVDDMKKTLGIKGKPVLVHGKRPFSFLKWLISSVTETIIRYYNSAP
                     ARYYNYKFMPNL"
     gene            1628..1846
                     /locus_tag="PK125_13070"
     CDS             1628..1846
                     /locus_tag="PK125_13070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix domain-containing protein"
                     /protein_id="HPB39076.1"
                     /translation="MSAQERVTIKIPKPLYNRISEIIEETGFSSVTDFIVYVLRDVIA
                     MRSEDSSRNLNRSEIESIRQRLKSLGYL"
     gene            1846..2802
                     /locus_tag="PK125_13075"
     CDS             1846..2802
                     /locus_tag="PK125_13075"
                     /inference="COORDINATES: protein motif:HMM:NF024257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HPB39077.1"
                     /translation="MKTTIVILFCIILVIVTFCPSPIVASSPTENLKGTIRISGAWAL
                     YPMMVKWSEEFRKMNANVRIDISAGGAGKGIADALAGMVDIGMVSRDIRPEEIKPGTT
                     YIPVVKDAVFPTISTNNPVLREISKKGMKKKTFKELWTIDKSLTWGEISGTSIKNAVQ
                     VYTRSDSCGAAETWAHYLGKKQEDLKGVGVYGDPGLADAVKRDAYGVGYNNLNYAYDA
                     KTGLPVAGIQIIAIDVNENGKIDTNEDLSTKEKAIKAVISGTYPSPPARDLYLITKEN
                     FKEPSKTFVKWILTDGQKYVSEVGYIKITQKQIIESLKKIGK"
     gene            2823..3713
                     /gene="pstC"
                     /locus_tag="PK125_13080"
     CDS             2823..3713
                     /gene="pstC"
                     /locus_tag="PK125_13080"
                     /inference="COORDINATES: protein motif:HMM:TIGR02138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HPB39078.1"
                     /translation="MDLRKFKDTFSTLSIRLSLVFVNTLAFVIAIILYIKAKPILVKV
                     SLSHLLFSTSWHPIRGDFGFFPFIIGTLGVTAIAMILAVPVCLLSAIYLSEYAHKRFR
                     AFVRLVVDILAGIPSVIYGLCGVIAIVPLVRFLGQAIGSPTTGYSLLAGGIILAIMVA
                     PVIISVALEVFRTIPQAARENSLALGITTWEMIKHVILRSSLHGIIAAIVLGFARAFG
                     ETIAVLMVVGNVAKIPDSFFAPAYPLPALIANNYGEMMSIPLYDSALMFSALLLMLVV
                     GGFSLAAHIILLRIERRGYA"
     gene            3710..>3914
                     /locus_tag="PK125_13085"
     CDS             3710..>3914
                     /locus_tag="PK125_13085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB39079.1"
                     /translation="MRARRIEETIFKGLMLASLVLVLIMLAGIIVITVYKGAPALTLS
                     MLVETPQGGYYLGKEGGIANAIVG"
BASE COUNT         1132 a          809 c          846 g         1127 t
ORIGIN      
        1 cttccgcctc gatatcctct atactctcct tgtgtgtgat ctgggaataa aggctaatga
       61 taatgcttct gctatatgtt cacggaccgg ttgcctttat agcgctctgt tgtctttgca
      121 aactggcatt atggattacg ttttaatgcc tctgagaata tggtgtgtat actctcttca
      181 tgggctacgc atcctttcca gaattcgggt ttcgctggct tcattatcct acaaacataa
      241 ggaacagatc actcatttgt gaagcttgtc ggatattact gataaaaagc gcttaatgac
      301 ataaattatg tttactctca actgttccct gattattata taggagctga gtggaggtca
      361 cagacagatg acacggataa aaaaagcttt tcttgtaatt ttcattctta ttgcagtaat
      421 ttttgtggtt tcttttctga ctggtatctt cggccgtgga ttaggcgaca agatcggggt
      481 tgtggagata gaaggcgtca ttactgatgg aaaagatact atggaggaca tagtcaaatt
      541 caaggaagat gacggcataa aaggtgtcat cgtcagaatc aactctcccg gagggtccgt
      601 tgggccaagt caggaaatct atagggaact gaagaaacta tctgtaaaga agaaggttta
      661 tatctctgcg agttcagtat gtgcttccgg tggatattat atcgcaagcg ctggagaaaa
      721 aatatttgct aacccatcaa caattaccgg tagcattggc gttatcatgg aacaggttgt
      781 tgctgaagat cttatgaaaa agattggtat ccaacctaat acaatcaaag caggacaatt
      841 caaggatgtg gggtcaccgt ttagaaaaat gaaggacgat gagagggcat attttcaaca
      901 gattcttgac agcatccatg aacagttcat taaagatgtt gcagaaggtc gcaagatgtc
      961 tgttgaacaa gcgaagaaac tttctgatgg tcgtgtatac actggttctc aggcaaaaga
     1021 acttggtctg gttgatagta tcggaacatt ttatgacact gtggacgaca tgaaaaagac
     1081 ccttggcata aaaggaaagc cggtacttgt ccacggaaaa aggccattct cgtttctcaa
     1141 gtggctcata tcttctgtaa ctgagacaat aatacgatat tacaattcag ccccagcgcg
     1201 ttattacaac tacaagttta tgcctaacct ttaaaatcaa aagcgagtcc tgatcagaac
     1261 aaaataaatt tggtttttaa aggctttcag caccttggaa aaatcatata cagaaaaaag
     1321 ggtatatttt tggtactctt cgaatttctg gtattatgcc ttttcttaat tttagtttat
     1381 aagggtaata aatttgttta taaaattgat ttcgcaacat aaggagaggc ggtatggtaa
     1441 acaccggtaa attcaattta ttccatcaat actcctgtac ctgcatgaga gtgaaaaaag
     1501 attctctaaa ccaatattta caatctatac gagacccgta tcattttttt ttacaattta
     1561 atcgggatcc attaaaaaaa tattgacaca ttaggacagg cctgctacac tcaaatatga
     1621 gccatatatg agtgcacaag aaagagtaac gataaaaata ccgaaaccgc tatacaacag
     1681 gataagcgaa atcattgaag agaccgggtt cagttcagta acagacttta ttgtgtatgt
     1741 attgcgggat gtcattgcaa tgaggtctga agactcatca agaaatttga accgatcaga
     1801 gattgaatcc attcgacaga ggctgaaatc attggggtac ctgtagtgaa aacaacaatt
     1861 gttatcttat tttgtatcat cttagttatt gtaacttttt gcccatctcc aattgttgca
     1921 tcatctccta ctgagaacct taaaggcacc attcggatat ctggtgcctg ggcactttat
     1981 cccatgatgg tgaagtggag tgaagaattt cgtaagatga atgccaatgt gcgcattgat
     2041 atttctgccg gaggggctgg aaaaggcata gcagatgcct tggctggcat ggttgatatt
     2101 gggatggtct ctcgggacat cagaccggaa gagataaagc cagggaccac atatattcct
     2161 gtggttaagg acgctgtctt tcccaccatc agtacaaaca accctgtgct cagggaaatt
     2221 agcaaaaaag gtatgaaaaa gaaaaccttc aaagagctct ggactattga caagtccctc
     2281 acctgggggg aaatatcagg aacctcgata aagaatgctg tgcaagttta cacccgctct
     2341 gattcctgcg gggctgccga gacgtgggcc cactacctgg gcaaaaaaca agaagacctt
     2401 aaaggtgtag gcgtatacgg tgatccaggc ctcgctgatg cggtgaaacg cgatgcttat
     2461 ggtgtgggtt ataacaatct caattatgcc tacgatgcca agacaggcct tccggttgct
     2521 ggcatccaga taatcgcaat cgatgtcaat gaaaacggca agattgatac taacgaagat
     2581 ctcagtacaa aagaaaaagc aatcaaagca gtcatctctg gtacataccc ttctccacct
     2641 gcccgtgacc tctaccttat aacaaaagaa aactttaaag aaccctcaaa aacctttgta
     2701 aaatggatat tgaccgatgg acaaaaatat gtcagcgagg tgggttatat taagatcacg
     2761 caaaaacaga tcatcgagtc tttgaaaaaa ataggaaaat aaggagatag gaaaatcttt
     2821 ttgtggatct aagaaaattc aaagacactt tttctaccct atctatccgg ctatcactgg
     2881 tttttgtcaa tactttagct tttgttattg ccattattct ttacataaaa gcaaagccta
     2941 ttctggtcaa agtatctctc tcacaccttc tcttttccac gtcttggcat cccattagag
     3001 gtgactttgg attttttcca ttcattatcg gtaccctggg ggtaactgca atcgctatga
     3061 tccttgcagt gccagtttgc ctgctgagcg ccatctacct ctctgaatat gcccacaagc
     3121 gttttcgagc ttttgtgagg cttgttgttg acatccttgc aggtattcca tcagttatct
     3181 atggtctctg cggggttata gccattgttc ccctggtaag atttcttgga caagccatag
     3241 gtagccctac caccggttat agcctgctcg ctgggggtat catcctcgct atcatggttg
     3301 cacctgttat catctcggta gctcttgagg tcttcagaac cattccccag gcagcccgcg
     3361 aaaattccct agcccttggt atcaccacat gggagatgat aaagcatgta atcctaagaa
     3421 gctctctcca tggaattatc gcggccatag tccttgggtt tgcccgtgca tttggagaaa
     3481 ccatagcggt tctcatggta gtgggcaacg tggcaaagat acctgattcc ttctttgccc
     3541 ctgcttaccc cctccccgct cttattgcaa acaattatgg agagatgatg tccataccac
     3601 tctacgactc agctctcatg ttttccgcac tcctccttat gctcgttgtt ggtggcttta
     3661 gcctagcagc tcatatcatc ctgctcagga ttgaacgaag aggatacgca tgagagcgag
     3721 acgcattgaa gaaaccattt tcaagggcct tatgctcgca tctcttgttc ttgttctcat
     3781 aatgcttgcc gggattattg tcattactgt gtacaagggg gcaccagccc ttaccctctc
     3841 aatgcttgta gagacaccgc aaggtggtta ttacctgggt aaagaaggtg gcatagccaa
     3901 tgcgattgtt ggat
//
LOCUS       DAOOMC010000186         3878 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdus sp. isolate AMR_MDS_2685
            NODE_28364_length_3878_cov_7.407795, whole genome shotgun sequence.
ACCESSION   DAOOMC010000186 DAOOMC010000000
VERSION     DAOOMC010000186.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864760
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdus sp. (wastewater metagenome)
  ORGANISM  Syntrophorhabdus sp.
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae; Syntrophorhabdus.
REFERENCE   1  (bases 1 to 3878)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3878)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,729
            Genes (coding)                    :: 2,708
            CDSs (with protein)               :: 2,708
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 7 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3878
                     /organism="Syntrophorhabdus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28364_length_3878_cov_7.407795"
                     /isolate="AMR_MDS_2685"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2699756"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            157..525
                     /locus_tag="PK125_13090"
     CDS             157..525
                     /locus_tag="PK125_13090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB39080.1"
                     /translation="MMKLRKVVGVIVVALFALNFIIPPGVFAQPQQNAEMEKKCAATK
                     AGTETDEAVRQSCATYFEQTEAAAAAGASGGEAATAGVSKGTVALTIIGIALLGGAAA
                     AAGGGGGGGGGGGAVPKHKK"
     gene            702..1910
                     /locus_tag="PK125_13095"
     CDS             702..1910
                     /locus_tag="PK125_13095"
                     /inference="COORDINATES: protein motif:HMM:NF014732.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNVR domain-containing protein"
                     /protein_id="HPB39081.1"
                     /translation="MEEKPIETQEEEINLLDYLIVLAKQKRFIIKVTSGFAIIAAIAS
                     LVMSPVYKAETKILPPVNISSGIASQLMNQLGQLGTIAGISGLPINGKAPNELYIGLL
                     KSRTVLDEMINRFDLMKLYGKRYRENAREKLINDVLTVRDDRKSGIITINIEDKDPKR
                     AAEMANAFVEELQKLNTGLAITQASQRRLYFEEQLKAAKTSLTQAEENMKGFQEKTGA
                     LHIESQAKAVLEGIGMIRAQIAAKEVEIKVMKTYSTPQNPDLKRVEETLKGLRAELAK
                     MEGKGGKGHDPLMPTGRMAQLGTDYAKQLRDLKFNEVLYGLLLQQYEAAKLDEGKNAT
                     TIQIIDKAVPPEKRVKPKRTAMVMLAAIIGFFLSAFAAFLIEYRERAKENPEDRARFD
                     TLKQYLSFKR"
     gene            2005..>3878
                     /locus_tag="PK125_13100"
     CDS             2005..>3878
                     /locus_tag="PK125_13100"
                     /inference="COORDINATES: protein motif:HMM:NF014606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLBB domain-containing protein"
                     /protein_id="HPB39082.1"
                     /translation="MNLKKTIIVFFLILSFEGSGFAQMAQMNPAKPNSEEITLEKPYN
                     TKDGIKDPQRISPEQLKQLGIKDSALPPQAQSVPSQKPEPDKPDQPKKVPSGQSSSQI
                     PAAESLSEFELFIARGTMGKISTNIRQFGYDLFRQPPSTFAPADRIPVGPDYVIGPGD
                     ELRITIWGNIEGQWTVVVDMDGKITLPKVGTIGVTGLTFGELKTTLNKELSRYYTNFQ
                     MNVSMGPLRSMRVYIVGNAAKPGAYTISSLSTLVNSLFEAGGPNKTGSMRDIQVKRNT
                     HTVVRFDMYDFLLKGNKTKDVRLMPEDVIFIPPVGPLAGIAGNVKNPAIYELKGETRL
                     LDLIEMAGGLSGMAFRGRVQVQRTQTNEFRTVFEGDLIDVEKNAEKNFLIKDGDLIKV
                     FSVADLKNTVTITGAVQNAGEYGVESGVTRVRDIISMSGGLQYYASSQMEITRVKVTQ
                     SGPQTERFVIDAFKAMEGDPANNVLLEMNDYLFVRTVPEWQLYRTVIVGGEVKYPGMY
                     TITRGERLSSVLERAGGFTNFSHLRGAVFTRERVRQLQQKSLEELAERLERELITESA
                     SRTGAAVSVEEVEAKKLEMEQRKQFIESLKKVKATGRMTIWLAHLRLLKGSEYDIELE
                     "
BASE COUNT         1221 a          774 c          983 g          900 t
ORIGIN      
        1 tgtattgatc aaaaacactc agatacaaac gattacgaag gatgaacccc ataattatct
       61 tgacaaaggc ttagggcagt gtaaaatgta aacgtgttat agggtgagta cttacaaaca
      121 tgatatgtac caaatccagt taggaggaga aagatcatga tgaagttgag aaaagttgta
      181 ggcgttattg ttgttgcctt gtttgcgctt aattttatta ttcctcctgg tgtttttgca
      241 cagccacaac agaatgcaga gatggagaag aaatgtgcag ccactaaagc tggaacagaa
      301 accgatgagg cagtcaggca gtcatgcgct acatactttg aacagaccga ggctgctgcc
      361 gcagcaggag cgagtggagg tgaagcagca actgctggcg tgagcaaggg taccgtcgct
      421 cttaccatca taggcattgc acttttaggc ggagctgcag cagccgctgg cggcggtggc
      481 ggcggtggtg gtggaggtgg cgctgtacct aagcataaga aataaaacag agaaactgtt
      541 taaccggatg cgctcactga caaagtatta aactgccata tttagatata caattctgaa
      601 ctaaatttct ctccaaagta gcagtcacgt ttacctgagt tccatttaaa atatgatttt
      661 gatgtatatc gttaaatcag cctgagcagg aggaaaaaag ggtggaagaa aaacctatag
      721 aaacccagga agaagaaatc aatctcttgg attacctcat tgttcttgca aaacagaaga
      781 gatttattat taaggtaact tctggtttcg caattatagc tgcaattgcc agtcttgtca
      841 tgtccccggt atacaaagct gagacaaaga tactgccgcc ggtgaatata agttctggta
      901 ttgcatctca acttatgaac cagttgggac aactgggtac catagccggt ataagtggtt
      961 tgccaatcaa tggaaaggca ccaaacgaac tctatatcgg tttgttgaag agcagaactg
     1021 tccttgacga aatgatcaac aggtttgacc ttatgaagct atacgggaaa agataccgtg
     1081 agaatgcgag ggaaaagctt atcaatgatg tgctgacagt tcgcgatgac aggaagagcg
     1141 gcatcataac aatcaacata gaggataaag acccaaagag ggcagcggag atggcaaatg
     1201 cctttgtaga agaattgcag aagcttaata ctggtcttgc cataacccag gcgagccaga
     1261 gaagactcta ctttgaggaa cagttaaaag cagcaaaaac ctctctaact caggctgaag
     1321 aaaatatgaa agggttccag gaaaaaacag gggcacttca tattgaatca caggctaagg
     1381 cggttcttga aggtatagga atgattcgtg ctcagatcgc agcaaaagaa gtagagataa
     1441 aagtaatgaa gacatactct acccctcaga atccagactt aaagagagtg gaggagaccc
     1501 taaaaggtct aagggcagaa cttgcaaaga tggagggtaa gggtggaaag gggcatgacc
     1561 ctcttatgcc aacaggcagg atggctcagc tcggaactga ttatgcaaag caactaagag
     1621 acctcaagtt caacgaagtt ctttatggac tgcttctcca acagtatgaa gcagcgaaac
     1681 tcgatgaggg gaagaatgcg accaccatcc aaataattga caaggcagta ccccccgaga
     1741 aaagggttaa gccgaagcga acagcaatgg tcatgctggc cgccattata ggttttttcc
     1801 tttcagcgtt cgctgccttt ctgattgaat accgtgagcg ggcaaaagaa aacccagaag
     1861 accgcgcacg attcgacaca cttaaacagt atttgagctt taaacggtaa aaggagtgaa
     1921 aagcaagaag taagaagtaa taaaaagctt acaagctatc gagcgggtaa gctatcaagc
     1981 aatacgataa acgaggtaga tatcatgaac ctaaagaaga ctataattgt gttttttctg
     2041 atcctttctt ttgaggggag tgggtttgcc cagatggcac agatgaaccc tgcaaagcca
     2101 aactcagaag aaatcactct ggagaagcca tataatacaa aagatgggat aaaagatcca
     2161 caacgcatat ccccagaaca gctgaagcaa cttggaataa aggacagcgc tctccccccg
     2221 caggctcaat ctgttccgag tcaaaagcca gagccagata aacctgacca gccaaagaag
     2281 gtgccatcag gacaaagctc ctcacaaatt cctgcagccg agagcctgtc ggagtttgaa
     2341 cttttcatag ccaggggaac catgggtaaa atttcaacaa atattcgtca gttcggttac
     2401 gatcttttcc ggcaaccacc gtcaaccttt gcccccgctg atagaatacc tgtcgggcct
     2461 gattacgtta ttggaccggg ggatgaactg cgaataacca tctgggggaa catcgaagga
     2521 caatggactg ttgttgtgga catggatgga aaaatcaccc tgcccaaggt tggtacaatt
     2581 ggcgtaacag gacttacttt tggtgaactt aaaacaaccc tcaacaagga actttccagg
     2641 tactatacta attttcagat gaatgtaagt atgggcccgt tgcgaagcat gagggtctat
     2701 atcgtgggaa atgccgcaaa acccggagct tacacaattt catccctttc aaccctggta
     2761 aattcgcttt ttgaggctgg tggaccgaac aaaactggat cgatgagaga tattcaggtt
     2821 aaacgaaaca ctcacaccgt tgtccggttt gatatgtatg actttctctt aaagggaaac
     2881 aaaacgaagg acgtcaggct catgcccgag gatgtgattt ttatcccgcc tgtgggtcct
     2941 cttgccggta tagcaggaaa cgtaaaaaat cctgccattt atgagttgaa gggagagacc
     3001 agactccttg atcttattga aatggcagga gggctatcag gtatggcttt ccgtggaagg
     3061 gttcaggtac agcgaaccca aaccaacgag ttcagaactg ttttcgaagg tgacctgatt
     3121 gatgtagaaa agaatgctga gaaaaatttc cttatcaaag acggagacct gataaaggta
     3181 ttctctgttg ctgacctcaa gaatactgta accataacag gggctgtcca gaatgcagga
     3241 gagtatggtg ttgaaagtgg agtcaccaga gtcagggata ttataagcat gtcaggaggc
     3301 cttcagtatt atgcatcgag ccagatggag attacacggg tgaaggtaac ccaatccgga
     3361 cctcagactg aacgcttcgt aatcgatgcc ttcaaggcta tggagggaga tccggcgaat
     3421 aatgtgctcc ttgagatgaa cgattacctc tttgttcgca ctgtgcctga atggcagctc
     3481 tatcgaactg ttattgtggg gggagaagtt aagtatcctg gcatgtatac tattactcgg
     3541 ggtgaaagac tctcttctgt tcttgaacgg gctggaggtt ttacaaattt ttcccacctt
     3601 cgtggcgcgg tcttcacgag ggagagggta agacagctcc aacaaaaaag tttagaggaa
     3661 ctggcggaac gcctggagag agagttgatc acggaaagtg caagccggac aggtgctgct
     3721 gtgtcagtcg aagaagtgga ggcaaaaaag ctggagatgg aacagagaaa acagtttata
     3781 gaatctctga agaaagtgaa ggctacgggc aggatgacca tatggcttgc ccacctgagg
     3841 ctccttaagg ggagtgagta tgacattgag cttgaaga
//
LOCUS       DAOOMC010000187         3698 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdus sp. isolate AMR_MDS_2685
            NODE_30229_length_3698_cov_7.107878, whole genome shotgun sequence.
ACCESSION   DAOOMC010000187 DAOOMC010000000
VERSION     DAOOMC010000187.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864760
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdus sp. (wastewater metagenome)
  ORGANISM  Syntrophorhabdus sp.
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae; Syntrophorhabdus.
REFERENCE   1  (bases 1 to 3698)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3698)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,729
            Genes (coding)                    :: 2,708
            CDSs (with protein)               :: 2,708
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 7 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3698
                     /organism="Syntrophorhabdus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30229_length_3698_cov_7.107878"
                     /isolate="AMR_MDS_2685"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2699756"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..977)
                     /locus_tag="PK125_13105"
     CDS             complement(<1..977)
                     /locus_tag="PK125_13105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB39083.1"
                     /translation="MKKTETNIKICTRCILPETFPNITFDSDGICNHCRKEEPVLARA
                     PARKADYRQKLDDLVASTRGKAPSYDAIMAYSGGKDSSYTLKLLRERYDLRILALTFN
                     NHFVSPTAFENIRAVTDALQVDHISFKPPWPLMKSLFGLTAQEDIFSAATLLRASSIC
                     TACITFVKSLTLKLAIEMSIPLSAFGWSPGQAPIQSAIMKTNGPLIRQNQKTLSASLP
                     PELRSQVASYLLPPAYYDLYKDRFPTNIHPLAFFDYDEEKIKGELESIGWEAPTNTDT
                     NSTNCLLNAFANQVHLERLKFHPYVWEIANMVRQGVVPREEGIEKIYTP"
     gene            complement(1125..1457)
                     /locus_tag="PK125_13110"
     CDS             complement(1125..1457)
                     /locus_tag="PK125_13110"
                     /inference="COORDINATES: protein motif:HMM:TIGR02436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HPB39084.1"
                     /translation="MVYQKSMEFSISVINVIDNIKTPRKHYRLIEQLESSCTSIALNI
                     AEGKGRFSKKEFKQFLYISRGSLYETITMLDIFERKKWLDEETYFGLYEKADEINRML
                     SGLIASLS"
     gene            complement(1531..3540)
                     /gene="asnB"
                     /locus_tag="PK125_13115"
     CDS             complement(1531..3540)
                     /gene="asnB"
                     /locus_tag="PK125_13115"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HPB39085.1"
                     /translation="MCGIAGIIDFSGRKPDEAMLRRMLGLIRHRGPDAFGIYMDDIAG
                     LAFTRLSIIDLHGGDQPIHNEDQTLWIVCNGEAFNYIELRPDLEARGHKFYTNTDTEV
                     LLHLYEEKGPEMLNDLNGQFAMAIWDKNRKELFLVRDRMGIRPLFYTKVGSRIIFSSE
                     IKAILADLTIPRTLDPVTLSDIFTCWSPFGDKTAFEGIQQLMPGHYALIGADGMKTRQ
                     YWKLTFEHNTNSDRPLHEWKEELKGLLQDACRIRLRADVPVGSYLSGGLDSTYTTSIV
                     KKIFNNHLCTFSVSFADSRFDEAFFQAKAVQAIGTDHKMIHCTDVDIGKDFPDVLWYT
                     ETPLLRTAPVPLFQLSKLVRENNYKVVLTGEGSDEIFAGYDIFKEDRIRRFWSRQPSS
                     ATRPRLLQRLYPDIFQQSDARSRAFLEGFFRKNLSFVDSPGYSHMIRWNNTALLQGFF
                     SSTLLSTLSGPSHPTTQRPNDFFGRFAASLPPDFMTWNPLSRAQYTESTIFLTNYLLS
                     SQGDRMAMAHAVEGRFPFLDYRVVEFATRVPPTYRLMGLKEKFILREAARDVIPPELA
                     NRPKHPYRAPISKCFFGDQHLQYVDDLLSESSLKEKGYFHPEKVAKLVAKVRRQDGQL
                     LSERENMGLIGILSTQLLDEMFIKNFPYRPIIEPEKVKVYDFRAK"
BASE COUNT          951 a          807 c          925 g         1015 t
ORIGIN      
        1 tgtggggtgt agattttttc gatcccttct tctctgggca caaccccctg gcggaccatg
       61 tttgctattt cccacacata ggggtggaac ttcaggcgct caaggtgtac ctggttggcg
      121 aacgcattga gcaaacaatt ggtcgagttc gtgtctgtat ttgtgggcgc ctcccaacca
      181 atgctttcga gttctccctt aatcttctct tcatcgtaat cgaaaaaggc aagggggtga
      241 atgttggtag gaaacctgtc cttatacaaa tcgtaatagg caggaggtag gaggtaggag
      301 gcgacctgcg aacgcaactc agggggaaga ctggccgaca aggttttctg attctggcgg
      361 atcagggggc cgtttgtttt cattatggcc gactggatag gggcctggcc aggtgaccag
      421 ccaaaagcag aaagcggaat cgacatctct atggccaact taagggtaag gcttttaaca
      481 aaagtgatac acgccgtgca gatagagctt gcacggagca gggttgccgc ggaaaatatg
      541 tcctcctgag ccgtgaggcc gaagagggac ttcatcaagg gccacggtgg tttaaaactg
      601 atgtgatcaa cctgaagtgc gtcggtgaca gcccggatat tctcaaaggc ggttggtgaa
      661 acaaagtgat tgttgaatgt taaggcgaga attcgcaagt cataacgctc acgtaaaagc
      721 tttaaggtat agcttgaatc tttacctccg ctgtatgcca taatagcatc gtaggaggga
      781 gctttccccc tggtggaggc cacaaggtcg tcaagcttct gccgatagtc agctttcctg
      841 gcgggagccc tggctaacac aggctcctcc tttcgacagt ggttgcagat gccatcggaa
      901 tcgaatgtaa tattggggaa ggtttcaggg agaatgcagc gagtacatat ttttatgttg
      961 gtttcagttt ttttcatcaa atttcatcct tgctatgtat ttccggatat gaagaatgtt
     1021 taaggtagga ggtacgctcc gcttgaggta acaaagtttc aaaatatcct ccagagtagc
     1081 agctgatagc tgtaagctgt aagctcatag taaaagctga gttattaact tagtgaagca
     1141 atcaacccag aaagcattct gttaatctcg tctgccttct catataatcc aaaataagtt
     1201 tcttcatcca accatttttt tctttcaaag atatcaagca tggttatagt ttcataaaga
     1261 gaaccgcgtg aaatatacag gaattgctta aactctttct ttgaaaatcg tccttttccc
     1321 tcggcaatat tcaaggcgat tgaggtacag gaagattcga gttgttctat aagccggtaa
     1381 tgctttcgtg gcgtttttat attatctata acgtttatta ccgaaatgga aaattccatt
     1441 gacttttgat aaaccatcaa atcctcgaat gcaaacccca tctgattctt ccctatgagc
     1501 tatgagctat gagctttgag cttacagcta ttacttagct ctaaagtcat acactttcac
     1561 cttctcgggc tcgattattg ggcgatatgg aaagttcttg atgaacattt cgtccagtaa
     1621 ctgtgttgag aggatgccga tgaggcccat attttcccgc tcgcttagaa gttgcccatc
     1681 ctgtcgacgc accttggcca ccagttttgc cactttttcg ggatggaaat accctttttc
     1741 tttaaggctt gattcagaaa gaagatcatc aacatattgc aagtgttgat caccaaagaa
     1801 acacttgctt ataggtgcac ggtacgggtg ttttggtcta tttgcaagct caggtggaat
     1861 cacgtctcgc gctgcttcac gcaatataaa tttctccttg agtcccatga gtctgtaggt
     1921 tggtggtacc ctggtagcaa actcaacaac acgataatcg agaaaaggaa accggccctc
     1981 cactgcatgg gccatggcca ttctgtcacc ctgggatgag aggagataat tcgtcagaaa
     2041 tatcgtactt tccgtatatt gggccctgga aaggggattc catgtcataa agtctggagg
     2101 caacgaggct gcgaaccggc caaaaaagtc gttgggtcgt tgggtcgttg ggtgagatgg
     2161 ccctgagaga gttgacagca gggtcgagct aaagaaacct tgtaaaagag ctgtgttgtt
     2221 ccaacgaatc atgtgggaat atccaggaga atcaacgaat gagagattct tgcgaaaaaa
     2281 tccttcaaga aaggctcttg atctcgcgtc tgactgttga aaaatatcag gatagagtct
     2341 ttggagaagc cttggccggg ttgctgatga aggctgccga gaccagaaac gccgtattct
     2401 atcctcttta aagatgtcat acccagcaaa aatctcatca gacccttccc ctgtcaacac
     2461 tactttgtaa ttattttccc ttaccagctt tgaaagttga aataatggga caggggctgt
     2521 ccgaagaagg ggggtttcgg tataccatag tacgtcagga aaatcctttc caatatcgac
     2581 gtcagtacaa tgaatcattt tatggtctgt tccgatggcc tgaactgcct ttgcctgaaa
     2641 aaaagcttca tcaaaacggc tgtctgcaaa acttactgaa aaagtgcaga ggtgattgtt
     2701 gaatattttc tttactatag acgttgtata tgtgctgtcg aggcctccac tcaaatagct
     2761 tcctacgggc acatcagcac ggagacgaat gcgacaggca tcttgaagaa gccctttgag
     2821 ttcctccttc cattcatgga ggggccggtc actattcgta ttgtgctcga acgtgagctt
     2881 ccagtattgc ctggttttca ttccatcagc accgatgagc gcatagtgtc caggcataag
     2941 ctgttggatg ccttcgaatg ctgttttgtc tccaaatggc gaccagcagg taaagatatc
     3001 agaaagagtc accggatcca gggtccgggg aattgtcagg tctgcgagga tagcttttat
     3061 ctctgaagaa aagataatgc gagatcctac ttttgtataa aagagcgggc ggatacccat
     3121 gcggtcacgg acaagaaaca actccttcct gtttttgtcc caaattgcca tggcaaattg
     3181 accattgagg tcattcagca tctctgggcc tttttcttca tagagatgga gaagaacctc
     3241 tgtatctgtg ttggtgtaga atttgtgtcc acgtgcctca aggtcagggc gtaactcaat
     3301 gtagttgaat gcttctccgt tgcatacgat ccacagagtc tggtcctcgt tgtgaatcgg
     3361 ctggtcacca ccatggaggt ctatgatgct aaggcgggtg aatgcgagcc ctgcgatgtc
     3421 atccatgtat atgccgaaag catcagggcc gcggtgacga ataagcccaa gcatcctgcg
     3481 aagcatggcc tcgtctggct tgcgccctga gaagtcaatt attcctgcta ttccacacat
     3541 aggttagtta gtcgagttaa gggagtctgt cgggtcgtcg ggtcgttggg tcatagggtc
     3601 atagggtcgt tgggtcgttg ggtcacaaac catcgtttct cgcccactca tcccgtcttt
     3661 gacgggactc gttcgagccg cagagggcgc agaggaag
//
LOCUS       DAOOMC010000188         3618 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdus sp. isolate AMR_MDS_2685
            NODE_31128_length_3618_cov_5.353915, whole genome shotgun sequence.
ACCESSION   DAOOMC010000188 DAOOMC010000000
VERSION     DAOOMC010000188.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864760
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdus sp. (wastewater metagenome)
  ORGANISM  Syntrophorhabdus sp.
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae; Syntrophorhabdus.
REFERENCE   1  (bases 1 to 3618)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3618)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,729
            Genes (coding)                    :: 2,708
            CDSs (with protein)               :: 2,708
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 7 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3618
                     /organism="Syntrophorhabdus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31128_length_3618_cov_5.353915"
                     /isolate="AMR_MDS_2685"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2699756"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..208
                     /locus_tag="PK125_13120"
     CDS             <1..208
                     /locus_tag="PK125_13120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB39086.1"
                     /translation="RQTMAQRTSGIFINISRRTVSFIPGVRKINAYIERYHRTVQEEF
                     IDHHIHLLPDEELFNKELAKYLIF"
     gene            455..1081
                     /locus_tag="PK125_13125"
     CDS             455..1081
                     /locus_tag="PK125_13125"
                     /inference="COORDINATES: protein motif:HMM:NF025774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MEDS domain-containing protein"
                     /protein_id="HPB39087.1"
                     /translation="MAHNCKIPLGFTDGEFPPGIHMCYIYADEEERRRVIFRFLESGL
                     LNGEKVSYFMDADTEDTMKEQRAVLGIDTLYQEQQEHLSLASTKDAYYPTGRFVPDDM
                     LCLLSDLYTQSRDAGYTGTRASGEMSWATRDISGSSRLMEYEALLNHTLKKYPITAIC
                     QYNAKRFNNSVLRDVLAVHPMIILHGRVLKNPYYIKSWWLLKKYLERK"
     gene            1327..2751
                     /locus_tag="PK125_13130"
     CDS             1327..2751
                     /locus_tag="PK125_13130"
                     /inference="COORDINATES: protein motif:HMM:TIGR02913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB39088.1"
                     /translation="MSLLVRRLIYAFVVVMTVALSQPTNAQKYAITDLGTLGGTESFA
                     YAINDSGDIIGYARYQGDTTSGPFLWSNGKMTDLNAYYGPEQGFYAGIMGNINNLGQI
                     VGNSSDGHAVMLSQGEVTDLGTFGGDYASALGINDLGQIVGYFALPSGHHHAFLYTDG
                     VMTALGPFGEEAISVATAINDSGMITGFATDSYMVSARAFLYNNGIMTDISPFDSRES
                     YARDINNHGQVAGEYLPYSGPFRCFVRSEHLISDLGTLAGIDCVALAINDQGEVVGSS
                     PAVVGTEISCDFETGMCYEYPVYSWHGFLYKNKRMIDLNELIPSDSGWELEWAYDINN
                     KGQIVGYGTFGDPSVYRAFLLSPVTRTTIRIKPSHVFNKINFKRGKKIPVAILSSESF
                     DAPGLVDKSSLTFGAVGDEASLIGCDRKQKDVNGDGLKDLVCKFSVRLAGFQCGDTEG
                     ILKAKMIDGTPIEGKDSVIIIPCK"
BASE COUNT         1009 a          730 c          829 g         1050 t
ORIGIN      
        1 cagacagaca atggctcaga gaacctcggg gattttcata aatatctcaa gaaggacggt
       61 atcctttatc cccggtgtcc gaaagatcaa tgcctacatt gaacggtacc acagaaccgt
      121 ccaggaggag ttcattgacc atcatataca tcttcttcct gatgaagagc tcttcaataa
      181 agaacttgcc aagtacctta tcttctagaa taccaaaaga gttcataaag gattcggcaa
      241 tataatgacg cccatccagt atcttatcga tgagggggta ttgtcgcaaa tgtgtttgac
      301 ttatacaata ggttgacatc tcagcaaaag gtgctataac acataataag aggacagtat
      361 agtaggactc gcaatcagat aggatatcgt ggctttatcc cgttcgtcgc atcaacgttc
      421 gtaaacaccg tttctccagt ttagttagaa acatatggct cataactgca aaattccctt
      481 gggatttacc gatggagagt ttccgcctgg tattcacatg tgctacattt atgccgatga
      541 ggaagagcgg agacgggtta tcttccggtt ccttgaaagc ggtttactca acggggagaa
      601 ggtgtcttat ttcatggatg ccgacacaga ggatacaatg aaggaacaac gggctgtgct
      661 cggtatagat acgctatatc aagaacagca ggaacattta tcattggcat caacgaagga
      721 tgcatactac ccgactggaa gattcgtccc tgatgatatg ctttgcctgt tgagtgactt
      781 gtacacacaa agtagagatg caggttatac tggtacgagg gcatcggggg agatgtcatg
      841 ggcaacacgg gatatatctg gttcttcccg acttatggaa tatgaagccc ttttaaacca
      901 caccctaaaa aaatatccga tcactgcaat ctgtcaatac aatgcaaagc ggttcaacaa
      961 cagcgtgtta agagatgttc tagccgtcca ccccatgata attctccatg gtcgggtact
     1021 taagaatccc tactacataa agtcttggtg gcttctgaaa aaatacttgg aacgaaaata
     1081 gattcttttc atcgaggaag atagagataa tctggtcctt cctcaatgtg ggcattgaca
     1141 aataacatat ctcatttata atacactaat gaagaaaagt ttagtcattt taaagttatg
     1201 ttattcccat agcttttctt gcacggcttg gtgattcgtg aaggttgaaa aaagacaaca
     1261 agtcagtcaa ccttgccatc tcgtctctgt atctcgttaa gtgcaataaa catgagggag
     1321 gaaaaaatga gtttgcttgt tcggcgcttg atatatgctt ttgtagttgt tatgacggtt
     1381 gccctttcgc aaccgacaaa cgcccagaaa tacgccataa ctgacctggg cactttagga
     1441 ggaacggaaa gttttgccta tgccatcaat gattcgggag atattattgg atacgctcgt
     1501 tatcagggtg atactacaag tggacccttt ctttggagta acggcaagat gaccgacctc
     1561 aatgcgtatt atgggcccga acaagggttt tatgcaggga taatgggaaa tataaataac
     1621 ctcggccaga ttgttggtaa ttcttcagac ggccatgctg ttatgctcag tcaaggcgag
     1681 gttactgacc tgggcacctt tggcggtgac tatgcgagcg cgcttggaat aaacgattta
     1741 gggcagatag taggatattt tgctctgcct tccggacacc accatgcctt cttgtacaca
     1801 gatggtgtta tgactgcgct cggccctttc ggtgaagagg ctatcagtgt tgccacggct
     1861 attaatgact ctggtatgat cacgggtttt gccactgatt catatatggt ctctgcacgt
     1921 gcctttcttt acaacaacgg gatcatgacc gacatcagcc cctttgatag cagggaaagc
     1981 tatgcacggg acatcaacaa tcacggacag gtcgctggtg aatatctacc ttatagtggt
     2041 cctttccgtt gttttgtccg aagcgagcac ctcatttctg atcttggaac gcttgccggt
     2101 atagattgtg ttgccctggc tatcaatgat cagggagaag tggtgggatc atcgcctgct
     2161 gtagtcggca cagagatttc ctgcgatttt gaaaccggaa tgtgttatga ataccctgtc
     2221 tattcttggc acggctttct ttataaaaac aaaaggatga ttgacctgaa cgaattgatc
     2281 ccttcggact ctgggtggga actggagtgg gcatacgata tcaacaataa ggggcaaatt
     2341 gttggatatg ggacatttgg tgatccgtct gtgtaccgtg cttttctatt atcaccagtt
     2401 accagaacca ccatcaggat caaaccatcc catgtattca acaagatcaa tttcaagaga
     2461 ggtaagaaaa ttccggttgc catactttcc tctgaatctt ttgacgcacc cggcctggtg
     2521 gacaagagtt ctctgacctt cggagccgta ggggatgagg ccagcctcat aggctgcgat
     2581 cgcaagcaga aagatgtcaa tggagatggt ctgaaggacc ttgtgtgcaa gttttcagta
     2641 aggctggcag ggttccagtg tggggatact gaaggtattc tcaaagcgaa gatgatagac
     2701 ggaacgccca tagaagggaa agactcagtc ataatcattc catgcaagta gactatttaa
     2761 cctgacgggc gagtagcccc aagcgttagg ttaagagttt atctatcgaa cagtgcaaga
     2821 ccgtctgcga gttatttctt aaaagatatt ctttgggtaa tttagctaga gatgccgtat
     2881 ttcacatgat atttcactat tgcccaagtt atgtggatgt tgattgatat ggcccatcac
     2941 agcaataatt tgctggacgt tctttttgtc cttcccgcgg ttttctacaa tctgaagcat
     3001 tcaaaccgcc ctgccttctt gattcttgca agcatggggc gtcttttttg acgctcgtaa
     3061 tttactgtgt ttaagcatca ttgtcaggga aaataagcta ttttttggca ccacgttttt
     3121 gaagatgtgt cactttacag gtggtgcatc caataaaaaa tcagggttat atgactggct
     3181 gttcaaacgg ttgttaaaag tttaggtcat aaagatcatc ctgcttgttt acagttttat
     3241 tctaattatc ttcggaatat tctttattag taaaagctat aattttattc gataaaaaac
     3301 ctgactgtta ttctataaat caatttgacg aatagacaat ctaaatgttt atcttccttt
     3361 gtttgaaatg acggctatag ttatcaaaat agcccatatg aatgcgattg gatgcaccgg
     3421 ggtcggggag ggtgctgcca actgaaggtt acaacccttt atgtataatc caataacaca
     3481 aacgtggtaa gggatcttat gggtttttta agaagcattg gaaaatcatt tcatgcggta
     3541 tatttattgg catcattgca actattcttc aaaagtttgg caaccctgca aacatgggta
     3601 tttgtgttgc ttgcttcg
//
LOCUS       DAOOMC010000189         3606 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdus sp. isolate AMR_MDS_2685
            NODE_31249_length_3606_cov_6.176852, whole genome shotgun sequence.
ACCESSION   DAOOMC010000189 DAOOMC010000000
VERSION     DAOOMC010000189.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864760
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdus sp. (wastewater metagenome)
  ORGANISM  Syntrophorhabdus sp.
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae; Syntrophorhabdus.
REFERENCE   1  (bases 1 to 3606)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3606)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,729
            Genes (coding)                    :: 2,708
            CDSs (with protein)               :: 2,708
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 7 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3606
                     /organism="Syntrophorhabdus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31249_length_3606_cov_6.176852"
                     /isolate="AMR_MDS_2685"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2699756"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            185..943
                     /locus_tag="PK125_13135"
     CDS             185..943
                     /locus_tag="PK125_13135"
                     /inference="COORDINATES: protein motif:HMM:NF021903.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HPB39089.1"
                     /translation="MDDIEGRIIQNKEIVSGHYLLKVKLAKPMNTPIPGQFAMMKLPD
                     SEVFLRRPFSIYDYRQDTAYFMYKVVGKGTHSLSRTGKNSTVSLLCPLGKGFNIQKRD
                     AYVVVAGGIGIAGVHLLIKRLGKQAIIFFGCNRAEELALMRYHINLNPYVSTLDGSYG
                     FHGNVVQLLSKHLKTICKSNIEVFTCGPENMFVSLRRLLEKMRIPCQASFEERMACGL
                     GLCFGCVKKTLDEKEPYKRVCKEGPVFDLWQVCL"
     gene            925..1827
                     /locus_tag="PK125_13140"
     CDS             925..1827
                     /locus_tag="PK125_13140"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: protein motif:HMM:NF005574.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HPB39090.1"
                     /translation="MASMSVKLFGKNLRNPVMNASGTLGYGEEIEPLWGIENLGAYVT
                     KGLSLNPHNGNPTPRVWEERQGLINSIGLQNVGVDRFFNEYFPLFKKKKTPIIVNFFG
                     FTEEEYVTCARRIKPDNLIIALEMNLSCPNVKQGGISFGKDPEMVYKIVKEVKAVTRI
                     PIIAKLTPEVRNLREIAGAAYDAGVDGFTLLNTMPSLVVDIEKCSVPIRGGLSGPVLR
                     PIALKAVYEIFSSLHVPILGSGGIMNYSDALSFIMAGACAVQVGTATFVDPYTIPKII
                     QGLKNYLEQLRCPRISDLTGVAHG"
     gene            1820..3520
                     /gene="recJ"
                     /locus_tag="PK125_13145"
     CDS             1820..3520
                     /gene="recJ"
                     /locus_tag="PK125_13145"
                     /inference="COORDINATES: protein motif:HMM:TIGR00644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008297 - single-stranded DNA
                     exodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HPB39091.1"
                     /translation="MDKGTEGAENKAQSVGPEIHGNPKVLAKELGIPDLAGRLLYSRG
                     IFDPDNADKFLYPKLKDLSDPFLLPDIDEGVGRTVEAIQKNENICLYGDYDADGVTSV
                     AILCNFFHHIGINPGIYIPGRYDGYGLNINAVKKLKEQGVSLLICLDCGSSNREEIEQ
                     AKSYGMDIIVIDHHEPPKARPAPVALINPKRADSRFPTRELAACGVTFFYVLALRRRL
                     AHMGLLTHPVNLKKELDLVVIGSMGDMVPLVNDNRILVKFGMATMRKSPRTWLKAFHS
                     ERILYNGVIDEYTLNFVVIPRINAAGRITDARRALDFLVSQDDTDACTLLVELNETNR
                     KRQKIEENILREISGSLANGDFDARKSIVLYNQSWHLGVVGIVAQKVMEKYGKPAIIL
                     TRVGDYLKGSGRGGNGIDLHGTVASLSPFLLKYGGHRFACGIALAEENLKLFIDAFEE
                     RIEAPITLPDKKYIVDACAGFEELTMEFMEFMEQLSPFGMGNPRPNLMLFPSALKQNN
                     RMVRISDNNNKIWFGSMQGQCEIPQNGPLGIIVTLTFKELRGEQFIHLNIKDIVSITG
                     "
BASE COUNT         1040 a          738 c          868 g          960 t
ORIGIN      
        1 tgagaaaatc ggcaaaagaa agaaatatta tacggaaact aatgaggatg cgttgatcat
       61 gtgcctatcg gtacgttagt tcatggttaa aatcctggca ctcggaatcg ggaaaataag
      121 gattgtggtc cccggttaac agtgaaggtt gaactgaaga ctgtaagcgt tattttatat
      181 agctatggac gatatagaag gcaggataat tcaaaacaaa gaaattgtat cagggcacta
      241 cctgttgaaa gtgaagctgg ctaaaccgat gaatacccct atacccgggc aatttgcaat
      301 gatgaagcta cccgacagcg aggttttcct gagaaggcca tttagcatct atgactacag
      361 gcaggacacg gcgtacttca tgtataaggt cgtgggaaag ggaactcatt cattgagcag
      421 aacagggaaa aattcaacgg tttctcttct ctgccccctc ggcaaggggt tcaacataca
      481 aaaaagagac gcatatgtgg ttgttgcagg gggtatcgga atagcaggag ttcatcttct
      541 gataaaacga ctgggtaaac aggctataat ttttttcggc tgcaacaggg cggaagaact
      601 ggcgctcatg agatatcata tcaacttaaa tccatatgtt tctacccttg acgggtcgta
      661 tggttttcat ggcaacgtcg tgcaactcct ttcgaaacat ttgaagacca tttgcaaatc
      721 aaacatcgag gtcttcacct gtggtccgga aaatatgttc gtaagcctca gaaggctttt
      781 agaaaaaatg agaatccctt gccaggcatc atttgaggaa cggatggcat gtggccttgg
      841 attatgtttc gggtgtgtaa aaaaaacact cgatgaaaag gagccttaca aaagggtttg
      901 taaagaaggt ccggtgtttg atctatggca agtatgtctg taaagctgtt tggaaaaaac
      961 cttcgaaatc cggtgatgaa tgcatcaggc accttgggat acggtgagga gattgaaccg
     1021 ctctggggaa ttgagaacct tggtgcctat gttaccaaag gcctgtcttt aaacccccat
     1081 aacggaaacc ccacaccccg cgtctgggaa gagcgacagg ggcttattaa tagcataggg
     1141 cttcagaacg tcggggttga caggtttttc aatgagtatt ttcccttgtt caaaaaaaag
     1201 aaaaccccga taatcgtcaa ttttttcggc ttcaccgagg aagaatatgt tacgtgtgct
     1261 aggcgcataa agccggacaa tcttatcatt gcccttgaga tgaacctctc atgcccaaac
     1321 gttaaacaag ggggcataag ctttggcaag gaccctgaaa tggtttataa gatcgtaaaa
     1381 gaggttaagg ctgtaacccg gattccaata atcgccaaac tgacccctga agttagaaat
     1441 ctccgtgaaa ttgccggggc tgcttacgat gctggtgttg atggatttac tcttttaaac
     1501 accatgcctt ccctagtagt tgatatcgaa aaatgttctg tccctatcag aggagggtta
     1561 tcaggaccag ttctcagacc cattgctctg aaggctgttt atgaaatttt ttcaagcctc
     1621 catgttccca ttctcggttc aggaggaatt atgaattata gtgatgcttt gtcttttatc
     1681 atggccggcg catgtgcggt tcaggtaggt accgccacat tcgtagaccc ctacacaatt
     1741 ccaaagatca ttcaaggatt aaagaattac ctggaacaat tgcgatgccc tcggatcagc
     1801 gatcttactg gtgtagcaca tggataaagg aacggagggc gcagagaaca aggcgcagag
     1861 cgttgggcct gaaatccatg gtaacccaaa ggtgttggcg aaagagctcg gcattccaga
     1921 tctagccgga cgccttctct actcgcgagg tatctttgac cctgacaatg ctgacaagtt
     1981 tctttacccg aaacttaaag atctctctga cccttttctt ttgcctgata ttgatgaagg
     2041 agttggaaga actgtggaag ccatacaaaa aaatgaaaat atctgtctct atggggacta
     2101 tgatgctgac ggagtgacat ctgtagctat cttgtgcaat ttttttcacc acataggtat
     2161 taatcctggt atctatatcc ccgggaggta tgatgggtat gggcttaata tcaatgccgt
     2221 gaaaaaatta aaagaacagg gtgtctctct tctcatatgc ctcgattgtg ggtcctctaa
     2281 cagagaagag atagaacagg caaaaagcta tggtatggat attattgtca ttgaccacca
     2341 tgaaccaccc aaggcacgcc ctgcccctgt tgcattgatc aatcccaaga gggctgactc
     2401 tcgttttccc acacgggagc ttgccgcgtg cggggtaacg tttttctatg ttctcgcctt
     2461 aagaagacgg ttggcccaca tggggcttct tacacaccca gtcaacctaa aaaaagagct
     2521 tgatctcgtc gttattggct ctatgggaga catggtccct ttggtaaatg ataacagaat
     2581 tttggtgaaa ttcggcatgg ctactatgag aaaaagtcct cgaacatggc tcaaagcctt
     2641 ccattctgaa cgtatcctct acaacggcgt catcgatgaa tatactctta attttgtcgt
     2701 tataccaaga atcaatgcag ctggaagaat tacagatgct cgtcgggcat tggattttct
     2761 tgtttcacag gatgacacag atgcctgtac attgctggtt gaacttaatg agacaaacag
     2821 gaaaagacag aaaatagaag aaaacattct tagggaaatc tctggtagcc tggccaatgg
     2881 agatttcgat gcaagaaaat cgattgtgct ttataatcag agctggcacc tcggggtagt
     2941 tggcattgtt gcccaaaagg tgatggagaa gtatggcaaa cctgcaatca ttctcacaag
     3001 agtaggcgac tacctgaaag gttcgggaag ggggggtaac gggatagacc tacatggtac
     3061 tgtggcttct ctttcaccat ttcttttgaa gtatggcggt cataggtttg cctgcggcat
     3121 agcgttggcc gaggagaacc tgaaattgtt cattgatgca tttgaagaac gaatagaggc
     3181 gccgattaca ttgcccgaca aaaaatatat agtggatgcc tgtgcaggat ttgaagaatt
     3241 gacaatggaa tttatggaat ttatggaaca actctcgcct tttggcatgg gtaatccacg
     3301 accaaacctc atgttgtttc catccgccct caagcagaac aaccgcatgg tcagaatttc
     3361 cgataacaac aacaagattt ggtttggttc catgcaaggt caatgtgaga ttccccagaa
     3421 cgggccgctg ggcattattg taacgcttac tttcaaggaa ctgagaggtg agcagtttat
     3481 ccaccttaat atcaaggaca tagtctccat tacaggttag ggacgtcgct cgttgtgatg
     3541 gtgtagatca ctcacagtca agacagtact cgcaatttat tgctgcaatg atcaatgata
     3601 attttc
//
LOCUS       DAOOMC010000190         3569 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdus sp. isolate AMR_MDS_2685
            NODE_31667_length_3569_cov_6.812749, whole genome shotgun sequence.
ACCESSION   DAOOMC010000190 DAOOMC010000000
VERSION     DAOOMC010000190.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864760
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdus sp. (wastewater metagenome)
  ORGANISM  Syntrophorhabdus sp.
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae; Syntrophorhabdus.
REFERENCE   1  (bases 1 to 3569)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3569)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,729
            Genes (coding)                    :: 2,708
            CDSs (with protein)               :: 2,708
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 7 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3569
                     /organism="Syntrophorhabdus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31667_length_3569_cov_6.812749"
                     /isolate="AMR_MDS_2685"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2699756"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1401
                     /locus_tag="PK125_13150"
     CDS             <1..1401
                     /locus_tag="PK125_13150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB39092.1"
                     /translation="KIQEKIFTLREGPPDDNYTFLFGVPFHTTSKNYKKGCEVPSPGG
                     KAFSLYIALKKLDENGMHGSLSWNARASDYQQALVKECGGTYQFVEPSPGRKYNVHCN
                     VTWQFGKIKPFIKIYHITEKKDIDITNGERDVLVGKKVKIKAVVLPYVDAGRGRWEGI
                     PDTAISGYIANDDTGKVLKFKDYDKSEIEFFFVEGKPEGKKVTLTYTADGGKVVGKTS
                     FQVFSPEVIMKELRPSKEITVGPVEKIKGKIGCWLYYGKLIKASPPAGEPGIMISHEI
                     KLPAKFSGHPHLLQHVQTVKEESLEYHDIDYFQMANKESCLDTYYPYGKMEPVGSPYK
                     DPAPNKIEMHDTPMNELGLATKEHHIRYQFQTFLMFIPSENPDDKNCAWIPLKVIEWD
                     WRAGIKRMVDWRKDPPCDKDTFKILYKYPANPTSKDCKKHPEWTCNVLNNQRKLIHGE
                     EAWKKAKSEQEKKPCN"
     gene            1422..1850
                     /locus_tag="PK125_13155"
     CDS             1422..1850
                     /locus_tag="PK125_13155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB39093.1"
                     /translation="MKIKKVFNIILSIVFIYAALVFASEFKVYPGAKIDEKTTKEANE
                     AAQGAKMSNVKSTIYTTMDSFPKVTLFYKSMSKEYIMPRASGTSGKPKKYEKYDLYEA
                     FFIFDDAKDLASSKLWVKIQRPYIGNDVRDITVILVTEKK"
     gene            1966..2910
                     /locus_tag="PK125_13160"
     CDS             1966..2910
                     /locus_tag="PK125_13160"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HPB39094.1"
                     /translation="MLNLRKITDNFSPSENIVLYPGDCLNLLKTIPDELLTLVITSPP
                     YNIGKEYEKKLKLEKYLEQQAAVIRECVRCLSPQGSICWQVGNYVDGGAIIPLDTVLY
                     PIFTKAGLRMRNRIIWHFEHGLHCSRRFSGRYETIIWFTKSDKYTFNLDPVRVPQKYP
                     GKKYFKGPNAGKYSCNPLGKNPGDLWVIPNVKSNHVEKTVHPCQFPVELIERFVLSMT
                     NEGDWVFDPFLGTGTTIVAAIRHNRRGAGAETVEKYVHLARQRIHGELVGTLRTRPMH
                     KPVYDPEGAGNSLRIAPWTKPDPKQMRLLEKKTGYTTK"
     gene            2907..3518
                     /locus_tag="PK125_13165"
     CDS             2907..3518
                     /locus_tag="PK125_13165"
                     /inference="COORDINATES: protein motif:HMM:NF020757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009036 - type II site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BglII/BstYI family type II restriction
                     endonuclease"
                     /protein_id="HPB39095.1"
                     /translation="MKIVETYSHLNGWEFLLVHQPNLWKEIQDVVAAVKAETCKTKVS
                     KEKTMKGKLLYSPVAMNSAFSQLLRSRLWEESRVSYWVTKNERLIRKTITMPAQEQKK
                     EIENAGETPIFSYNQTDFVKDRVAVEVQFGKYSFVAYDLFVKHLAFYVGDRIDVGIEI
                     LPMKSLQSEMSSGVAYCEGELYNVVRQGRGVPAVPLVIIGIAP"
BASE COUNT         1145 a          730 c          800 g          894 t
ORIGIN      
        1 aaaattcaag aaaaaatatt taccttacga gaggggcctc ctgacgacaa ttatacgttt
       61 ttgtttggag tcccattcca taccacgagt aagaattaca aaaaaggatg cgaagtaccg
      121 tcaccgggtg gcaaggcatt ttcgttatat atagcactaa agaaactgga tgaaaacggt
      181 atgcatggtt ccttgagttg gaatgcgaga gcttccgatt atcagcaggc tcttgttaaa
      241 gagtgcggag ggacctatca gtttgtcgag ccctctccgg gaaggaaata taatgtccac
      301 tgtaatgtta cgtggcagtt tggaaaaata aaacccttta tcaaaatcta tcacatcact
      361 gaaaagaaag atattgacat tacgaacgga gaaagagatg tgctggtagg aaagaaggtt
      421 aaaataaaag cggttgtgct tccatatgtt gatgcaggca ggggccgctg ggaaggcatt
      481 cctgatacag ccatctctgg ctatattgct aatgacgata caggtaaggt tttaaagttc
      541 aaagattacg ataaatcaga aattgagttt ttctttgttg agggaaaacc tgaggggaaa
      601 aaagtaactc taacttacac tgcagacggc ggcaaggtag tgggaaaaac gagctttcag
      661 gtcttctcac cagaagtaat aatgaaagaa ttacgacctt caaaagaaat cactgtgggg
      721 ccggttgaga aaataaaagg gaaaatagga tgctggcttt attatggaaa actcattaag
      781 gccagtcctc cagcgggtga acctgggata atgatttctc atgaaattaa attgccagcg
      841 aaattcagtg gtcatcccca tctccttcag catgtccaga cagtcaaaga ggaatcatta
      901 gaatatcacg atattgacta ttttcaaatg gctaataaag aatcatgtct ggatacttat
      961 tacccgtacg ggaaaatgga accggtgggt tctccctata aggaccccgc tcctaacaaa
     1021 attgaaatgc atgacacgcc tatgaatgaa ctggggttag caactaaaga acatcatatt
     1081 cgataccagt tccagacatt tctgatgttt atcccttctg aaaaccctga cgataagaac
     1141 tgtgcatgga ttcctctcaa ggtcatcgag tgggattggc gtgccggtat aaaacgtatg
     1201 gtggactgga gaaaagatcc tccgtgtgat aaagatacct ttaagatttt atacaaatat
     1261 cctgctaatc caacgagtaa ggattgtaaa aaacaccctg aatggacatg taatgtattg
     1321 aataatcaaa ggaaattgat tcatggtgag gaagcttgga aaaaagcaaa gtctgagcaa
     1381 gaaaaaaagc cgtgtaattg aatccaagtt taataaggac gatgaagata aaaaaggtgt
     1441 ttaacattat tctatcaatc gtttttattt atgctgccct tgtctttgct tcagaattta
     1501 aggtttatcc tggcgcgaag atcgacgaga agaccaccaa agaggcaaat gaggctgccc
     1561 agggcgccaa aatgtccaac gtcaaatcca cgatctatac aaccatggat tctttcccca
     1621 aagttacttt gttctacaaa agcatgtcaa aggaatatat aatgccccga gcttcaggga
     1681 cctctggcaa gcccaaaaaa tatgaaaaat acgatttata tgaggctttt ttcatctttg
     1741 acgacgccaa agacctggcc agttcaaaac tctgggtgaa aattcagagg ccctacatcg
     1801 gaaatgatgt gcgggacatc acagtcatcc tggtcactga gaagaaataa cggctaataa
     1861 taaaatccag cagacgccct ccagccgctg ctgattctgt tgttcgctga taaaaagaaa
     1921 tacgaaaaga ttgactatag aatcccctgt tgctatcata gaaacatgct taatctccga
     1981 aaaataacag ataatttctc accgtcagaa aatatagtgt tgtatcctgg tgactgctta
     2041 aatctgctga agacgatccc cgatgaatta ttaacacttg taataacttc gcctccttac
     2101 aatatcggaa aagagtacga aaagaaactg aagctggaaa aatatcttga acagcaggca
     2161 gcagtcattc gcgagtgtgt gaggtgtctt tctccacaag gtagcatttg ctggcaagtg
     2221 ggaaactacg ttgatggcgg agccattata cctctggaca ctgtgcttta ccctatcttt
     2281 accaaggcag gcttgaggat gcgaaaccgg atcatctggc acttcgaaca tggattgcat
     2341 tgcagtcgta ggttctcggg ccgctacgaa acgatcatat ggttcacaaa gagtgacaaa
     2401 tataccttca acctcgatcc ggttagagta cctcaaaaat atcctggcaa gaaatacttc
     2461 aaaggaccaa atgctggcaa gtattcatgt aatcccctcg gcaagaaccc cggtgactta
     2521 tgggtcattc cgaatgttaa aagcaatcac gtggaaaaaa cagttcaccc atgccagttt
     2581 cctgttgaac tcattgagcg atttgtactg tctatgacga acgaaggaga ttgggtattc
     2641 gatcccttct tgggaactgg cacaaccatc gttgctgcca ttcgtcataa tcgccgagga
     2701 gcaggagctg aaacggttga aaagtacgta cacttggcaa ggcagcgcat tcacggagag
     2761 cttgttggca ccctgagaac tcgcccgatg cataaacccg tatacgatcc agagggggcc
     2821 ggaaacagct tgcgaatcgc cccctggacc aaacctgacc cgaaacagat gaggctactc
     2881 gaaaaaaaga caggctatac aactaaatga agattgtcga aacttactca catctgaacg
     2941 gttgggaatt ccttttagtc catcaaccaa atctgtggaa agaaatacaa gatgtggtgg
     3001 ctgcggtcaa agcagaaacg tgcaagacca aggtgtctaa agaaaaaact atgaaaggca
     3061 agcttctcta tagccctgtt gccatgaatt ctgcgttttc acaactactc cgttcccgtt
     3121 tatgggaaga gagccgggtc agctattggg tcacgaagaa cgaaagacta atccgcaaaa
     3181 ccattacaat gccggctcaa gaacagaaga aagaaatcga aaatgccgga gagacaccta
     3241 ttttcagtta caatcaaacc gactttgtga aagatcgcgt tgccgttgaa gtccaattcg
     3301 gcaagtattc tttcgtagct tatgacttgt tcgtaaagca tttggctttt tacgtcggcg
     3361 accgtattga cgtgggcatt gaaatactgc ccatgaaatc tcttcagtct gaaatgagtt
     3421 ccggtgtcgc atattgcgag ggagagttat ataatgtcgt caggcaaggt cgaggtgttc
     3481 cagctgttcc cctcgttatt atcggaattg caccttaaat taaacccagc aaaaagtgcg
     3541 tacagacaca tctagcgtga aaattgagt
//
LOCUS       DAOOMC010000191         3533 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdus sp. isolate AMR_MDS_2685
            NODE_32091_length_3533_cov_4.723117, whole genome shotgun sequence.
ACCESSION   DAOOMC010000191 DAOOMC010000000
VERSION     DAOOMC010000191.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864760
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdus sp. (wastewater metagenome)
  ORGANISM  Syntrophorhabdus sp.
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae; Syntrophorhabdus.
REFERENCE   1  (bases 1 to 3533)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3533)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,729
            Genes (coding)                    :: 2,708
            CDSs (with protein)               :: 2,708
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 7 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3533
                     /organism="Syntrophorhabdus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32091_length_3533_cov_4.723117"
                     /isolate="AMR_MDS_2685"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2699756"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..324
                     /locus_tag="PK125_13170"
     CDS             <1..324
                     /locus_tag="PK125_13170"
                     /inference="COORDINATES: protein motif:HMM:NF028456.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB39096.1"
                     /translation="IDYLKDEGKEAGLVQLRLFRPFPKDDFLKAIEGKKRLAIIERAM
                     PGGATYGPLFNEIAALAHTHGLDIALENYICGLGGRDVQPEEFMDVFTSPQSGKNITV
                     LGVRE"
     gene            321..1247
                     /gene="porB"
                     /locus_tag="PK125_13175"
     CDS             321..1247
                     /gene="porB"
                     /locus_tag="PK125_13175"
                     /EC_number="1.2.7.1"
                     /inference="COORDINATES: protein motif:HMM:NF008819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019164 - pyruvate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate synthase subunit PorB"
                     /protein_id="HPB39097.1"
                     /translation="MRLKKIYSYTDHPEVEYAVSGHRACQGCAEILGLRLALKVFGKD
                     TIMTMATGCMEIISTPFPLTDWTIPWIHVAFENAAAVASGVESALKILMEKGKIPKKD
                     IHVIASAGDGGTSDIGLQALSGALERGHKFTYICVDNEAYMNTGIQRSSATPFGAMTT
                     TSPPGKKSMGQATWKKNMPKIAAAHNIPYVATACPSYPFDLMEKAKKAKNTNGPSYLH
                     ILSVCPTGWRIPTEAAIEYGRLAVRSGAFPLYEVENGKYRITQKTDRLIPISEYLKGQ
                     GRFRHLTDDIIENIQTRISAEWEELKFLSGIK"
     gene            complement(1265..2395)
                     /locus_tag="PK125_13180"
     CDS             complement(1265..2395)
                     /locus_tag="PK125_13180"
                     /inference="COORDINATES: protein motif:HMM:TIGR01420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HPB39098.1"
                     /translation="MEDLTWYLKYMVEKDASDIYITVGVPPMFRIEGVVYPYTGDVLE
                     PDDTEAMAYRAMNERQRKIFEETLEMNLALYYPELGRFRVNIFRQRGYVGMVIRQIKV
                     QIKTIDDLGLPQILKDIVMTKRGLVLVVGATGSGKSTSLAAMIDYRNLNQRGHIISIE
                     DPIEFVHEHKMSVITQREVGFDTHSFLNALKNTMRQAPDVILIGEIRDAETMEDAITF
                     AETGHLALATLHSNNANQAMERILNFFPIERHLQIYMQLSLNLRAIISQRLLPSIDGT
                     RVAAFEILLDTPRVKDLILKGEIGVLKDTMAQSYNEGMQTFDQHIFDLYMAGRIDYNT
                     AVAYADSPNDVRLRIKMSQIKKEDTERKDPGFKLKVDDFSKF"
     gene            complement(2400..3434)
                     /locus_tag="PK125_13185"
     CDS             complement(2400..3434)
                     /locus_tag="PK125_13185"
                     /inference="COORDINATES: protein motif:HMM:TIGR01420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HPB39099.1"
                     /translation="MDISELLIFAVENKGSDLHISSGEQPFIRINGEMRKVELPHLDR
                     EDVHNMIYGILNDFQRKTYEEHLELDFSFDLGGYGRFRCNVFKQQRGDAAVFRTIPSI
                     IPSFEQLGLPNVLKAVSRLEKGLVLVTGPTGTGKSTTLAAMIDLINNEFKSHIITIED
                     PIEFVHVSKNCLVNQRELGPHTHSFANALRSTLREDPDVILVGEMRDLETISLALTAA
                     ETGHLVFATLHTSGAPNTIDRIVDVFPAEQQNQVRSMIAESLQAIVSQVLFKKRDGKG
                     RVAAFEIMISNPAVRNLIRENKIFQIPSVMQTSRGIGMQTMESSVQDLVDRNLVSKDE
                     VSFYLSKVQR"
BASE COUNT          981 a          779 c          737 g         1036 t
ORIGIN      
        1 attgactatc taaaggacga aggaaaggaa gcaggccttg tgcagcttcg tctattccga
       61 ccatttccaa aagacgattt cctgaaggct attgaaggga aaaagcgcct tgcaattatt
      121 gaaagggcaa tgccgggcgg ggccacttac ggtcccctat tcaatgaaat tgcagccctt
      181 gctcataccc atggtcttga catagcgctt gaaaactata tttgtggcct tggaggtaga
      241 gatgtacaac cagaggagtt catggatgtt tttacgtctc cccaatcggg gaaaaatatc
      301 actgttttag gggtgagaga atgagattga aaaaaatata ttcatataca gatcatccag
      361 aagttgaata tgccgtgtca ggacatcgtg cctgtcaggg ctgcgcagag atattaggac
      421 ttcgtttggc cctgaaggtt tttggtaagg atactattat gaccatggcc actggttgta
      481 tggaaatcat ttctacccct tttcctctaa ccgactggac aataccgtgg attcacgtgg
      541 cctttgagaa tgctgctgca gtcgcctccg gtgttgaatc agcattaaaa atactcatgg
      601 aaaagggcaa gattccaaaa aaggacattc atgtcattgc aagtgccggt gatggcggga
      661 cgagtgatat aggtttgcag gcattgtctg gagctctgga acgaggtcac aaattcacgt
      721 atatctgcgt tgataatgaa gcttatatga atacaggcat acaaaggtca tctgctacac
      781 ctttcggcgc catgacgacc accagtcctc cgggcaagaa aagcatgggt caggccacgt
      841 ggaagaaaaa catgcctaaa attgcagcag cccataatat cccttatgtt gcaactgctt
      901 gcccctctta tcctttcgat ctcatggaaa aggctaagaa ggctaagaac acaaatggtc
      961 cctcgtacct tcacatcctt tccgtctgtc cgaccggatg gagaatacca acagaggctg
     1021 caatagaata tgggagactt gcagtaagaa gtggtgcatt tcctctctat gaggttgaaa
     1081 acggaaagta caggattact caaaaaactg atcgcctgat accaatatct gagtatctga
     1141 aaggccaggg gagattccgt caccttactg acgatattat tgaaaatatt cagacacgta
     1201 taagtgcaga gtgggaagaa ctgaagtttc tgtcggggat taagtaaggc tgcggagtta
     1261 attttcagaa tttcgaaaaa tcgtctactt taagtttgaa ccccggatct tttctctcag
     1321 tatcctcctt tttgatctga gacatcttta tgcgtaatct cacatcgttc gggctgtctg
     1381 cataagcaac agccgtattg taatcaattc tcccagccat atacaggtca aatatgtgtt
     1441 ggtcaaacgt ttgcatgccc tcattataag actgggccat tgtatctttt aaaacaccaa
     1501 tttcaccttt taaaatgagg tctttgaccc tcggcgtatc gagaagtatc tcaaaggctg
     1561 caactcttgt accatctatt gatgggagca gacgttgtga aattatagcc ctaaggttga
     1621 gagagagctg catataaatc tggaggtgtc tctctatggg gaaaaaattg agtatgcgct
     1681 ccattgcttg gtttgcatta tttgagtgca aggttgccaa cgcgaggtgt ccggtctcag
     1741 caaacgtgat ggcatcttcc atggtctctg catccctgat ttcaccaata agaattacat
     1801 ctggcgcctg cctcattgta tttttaagtg cattgagaaa gctgtgggtg tcaaagccca
     1861 cttcacgctg agtaatgacg ctcattttat gctcatgaac aaattcgatg gggtcctcaa
     1921 tgctaataat atgcccacgt tgattaagat ttctatagtc tatcattgca gcaagactcg
     1981 tcgatttacc actcccagtg gcaccaacaa cgagaacgag acctctcttt gtcatcacaa
     2041 tatccttcaa gatttgcggt aatccaagat catcaatggt tttaatttgt accttgattt
     2101 gacggataac catcccgaca taacctctct gacgaaagat attgactcta aatctaccta
     2161 gctctgggta atagagagcc aggttcattt cgagagtttc ttcaaaaatt tttctctgac
     2221 gttcgttcat tgcccgatac gccatagcct cagtatcatc aggttccaat acatcccctg
     2281 tatatggata cactacgccc tcaatacgaa acatgggtgg aacaccaaca gttatataaa
     2341 tatccgacgc atctttctca accatgtatt ttaaatacca agttagatct tccattttct
     2401 cacctctgaa cctttgagag atagaaagat acctcgtctt tgcttacaag gtttctgtca
     2461 acaagatcct gaactgagga ttccatagtc tgcattccta ttcctctgct ggtctgcatt
     2521 acagaaggaa tctgaaatat cttattttcc ctaatcaggt ttcttaccgc tggatttgat
     2581 atcataatct caaaggcagc tacacgccct tttccatctc tttttttgaa taagacctga
     2641 gaaacgatgg cctgtaacga ctcggcaatc attgaacgaa cctggttttg ctgttctgct
     2701 ggaaaaacat cgacgattct atctatcgtg tttggcgcac cgctcgtatg aagggttgca
     2761 aaaacgaggt ggcctgtttc tgcggctgta agggcaagag atatggtttc aagatctctc
     2821 atttcaccta caaggattac atctgggtct tcacgaaggg tgctccttaa tgcatttgca
     2881 aaactatggg tatggggccc caattccctt tgatttacga gacagttttt tgatacatga
     2941 acaaattcga tcggatcttc gatggtgatt atgtggctct tgaactcatt gttaataaga
     3001 tcgatcatcg cagcaagggt agttgatttt ccggtaccag ttggacccgt aacaagtacc
     3061 aggcctttct caagccttga tactgctttc aacacatttg gcaaaccaag ctgttcaaag
     3121 gaaggaatta tacttggaat tgtcctaaaa actgcagcat cacccctttg ctgcttgaaa
     3181 acattacatc gaaaccgacc ataccctcca agatcgaaag aaaagtcaag ttcaaggtgc
     3241 tcttcataag tctttctctg aaaatcattc aggattccat aaatcatgtt atgtacatct
     3301 tccctgtcaa gatgtggtaa ttcgaccttt ctcatctctc cgttaattcg tataaacggt
     3361 tgttctccag agctgatgtg aaggtctgat cctttgtttt ctactgcaaa gataagaagt
     3421 tctgatatat ccacgttatc cccctagttt tctaaggctt ctatttcttt taatgtcgaa
     3481 aatctccgat atccttaagc ccatatacct cgaggaatgt ctctgttgtt cta
//
LOCUS       DAOOMC010000192         3412 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdus sp. isolate AMR_MDS_2685
            NODE_33595_length_3412_cov_5.468871, whole genome shotgun sequence.
ACCESSION   DAOOMC010000192 DAOOMC010000000
VERSION     DAOOMC010000192.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864760
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdus sp. (wastewater metagenome)
  ORGANISM  Syntrophorhabdus sp.
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae; Syntrophorhabdus.
REFERENCE   1  (bases 1 to 3412)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3412)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,729
            Genes (coding)                    :: 2,708
            CDSs (with protein)               :: 2,708
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 7 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3412
                     /organism="Syntrophorhabdus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33595_length_3412_cov_5.468871"
                     /isolate="AMR_MDS_2685"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2699756"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..190
                     /locus_tag="PK125_13190"
     CDS             <1..190
                     /locus_tag="PK125_13190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB39100.1"
                     /translation="VGEYCIKGENLDFLNIGGTKINELFDGREYVSALHQVNRFEIIS
                     KQLKLYYIDTDYLLFKKK"
     gene            233..2203
                     /locus_tag="PK125_13195"
     CDS             233..2203
                     /locus_tag="PK125_13195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB39101.1"
                     /translation="MKKRVLLTRKCITMLLALLMLIQQGCDLFSLKLESTSDTGYEGF
                     QSDVELFYEFAYLKEAMFLEFLNYTSDGFTTGPLEGTLSMKECKRLVDYIADISEKEE
                     LYVMAAQQLEQSGVLQSPTRTKGLVSSITNFFGKVSGIQKRNRQRYMVVYTNVDRNVR
                     TKMYESAVKCWDTYDWAGKPRSEQEFYNRMLDGSYDGHGNRLMGDLLAMKDLDVQYTI
                     SEKGLTLQDLTKDYGEIATSGAQIALDAFSTIVPGSEIGMEIVSVTDNVDRLANAKDF
                     NEKAKAKEDMKNYIRGKVFGEIFGETTVEVSDFMCEQLAEHIERVSQEATREVDKKKQ
                     GQIVVQDQNKEQPAKIVISQKEESSPSNDGPSIFVTGKKTIKKGVDQLLTAGKWLITA
                     INEQGRRETVEVEVKAGKVIVVEVNTAEPVKKEDDDNEGDIADGNAYYLLGDIHFYTS
                     PDANTAKGVDLNVYHDSWHVSLRGARGYWLDEFSSCTEPSDFFVLKQKQSSKTQFSGR
                     GKVSFTKKVSALGRTYITNISEVTEMELTVDKAKNPTKITKFTLNHTSHQEGHYANES
                     TVYVFDEKSELAFKDVPISLLTKYTEKVDLPAKWMKSASYFYNTKGGVVFIKGSDLSP
                     YLVSATGTKGDESSKKLFAQNTIIMFYVVQKK"
     gene            complement(2293..>3412)
                     /locus_tag="PK125_13200"
     CDS             complement(2293..>3412)
                     /locus_tag="PK125_13200"
                     /inference="COORDINATES: protein motif:HMM:NF015764.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPB39102.1"
                     /translation="VSIFMPALIGIIADKWIPAQKLLGICHGIAAILMIATGWYGLTA
                     ETVQFGTIFTLYSFSVAFFMPTIALINSVSYNALEKAGMDTVKVFPSIRIWGTIGFIV
                     SMWCVDLMGIQASAYQYVFSGILGLILAGYSFTLTHCPVNKGGEKKSLVEAMGLKAFA
                     LFKDSRMAIFFIFSMLLGVALQITNSFANPFLGDFAGIEIYANTFGVKHSNILISLSQ
                     ISEALCILLIPFFLRRYGIKKVMLIAMFAWVLRFAFFGLGNPGNGVWLFVLSMLVYGV
                     AFDFFNISGSLFVDRNTDHSIRSSAQGLFMLMTNGIGAAIGTLGAQAVVNALVNSQTD
                     PIAKMTGWSHTWYIFAAYALVITIAFAILFKYEHVPEK"
BASE COUNT         1077 a          677 c          836 g          822 t
ORIGIN      
        1 agtgggggag tattgtataa aaggggagaa tcttgatttt ctaaacatag gaggaactaa
       61 aatcaatgaa ctttttgatg gacgagagta tgtgtcggct ctacatcaag tcaatcgatt
      121 cgaaataatt tcaaaacaat tgaaattata ttatattgat acggattatt tactgtttaa
      181 aaagaaatga gaatcacgct aacatcaaat tagcgtgagc aaagatgtta ttatgaaaaa
      241 aagagtattg ttgacacgta aatgcatcac tatgctgtta gcactgttga tgctgataca
      301 gcagggctgt gatcttttta gtttaaagtt agaaagtact tccgacacag gctatgaggg
      361 cttccaatct gacgtagaac tcttctatga gttcgcatat ctaaaggaag ccatgttcct
      421 cgagttcttg aattacacct ccgacgggtt cactacgggt ccgctcgagg gaaccttgag
      481 tatgaaagag tgcaaacggt tagttgacta cattgccgac ataagcgaaa aggaggagct
      541 gtatgtgatg gctgcgcaac aactggagca gtcaggagtg ctgcagtcgc ctacaaggac
      601 caagggactg gttagctcta taaccaactt ctttggcaaa gtttccggca ttcagaaaag
      661 gaacaggcag cggtatatgg tggtttatac caatgtggac agaaatgtac gcaccaaaat
      721 gtacgaaagt gccgtaaagt gttgggacac ctacgactgg gccggtaagc ctcgcagtga
      781 gcaagaattc tacaatagga tgctcgatgg gtcgtacgat ggacacggga atcggctgat
      841 gggtgacctg ctggccatga aagatcttga tgtacagtat acgattagcg agaagggact
      901 aaccctgcaa gacttgacga aagattatgg ggaaatagcg actagtggcg cccagatcgc
      961 actcgacgcc ttctccacaa tagtgcccgg ctcagagatc ggtatggaga tcgtctcggt
     1021 caccgacaat gtggaccggc ttgcgaatgc taaagatttc aatgaaaagg caaaagcgaa
     1081 agaggacatg aaaaactaca ttagggggaa agtctttgga gagattttcg gggaaactac
     1141 cgtggaggtt tcggatttca tgtgcgagca actggccgaa catatagaaa gggtatcgca
     1201 agaagctaca cgagaagttg acaagaagaa gcagggtcaa attgtcgttc aggaccagaa
     1261 caaggagcag ccggcaaaaa tagtgatttc gcaaaaagag gaaagctccc cctccaatga
     1321 tgggccgtcc atcttcgtga cagggaagaa aacgatcaaa aaaggtgttg atcagttgct
     1381 aaccgcgggg aaatggctta ttacagctat caatgagcag ggacgtcgcg aaacagtaga
     1441 ggtagaggtg aaagcaggga aagtaatcgt cgtagaggtg aataccgctg agccggtaaa
     1501 aaaagaagat gacgataatg agggcgatat tgctgacgga aatgcctatt acttgttggg
     1561 agatattcat ttttatacat caccggacgc caatactgct aagggagtag acttgaatgt
     1621 ttaccatgat tcctggcatg tcagtttaag gggggcaaga ggctattggc tagatgagtt
     1681 ctcctcctgc actgaaccct ctgacttctt tgtgctgaaa caaaaacagt cgagcaaaac
     1741 ccaattctcc gggcgaggca aggtttcatt tacaaagaaa gtctccgcat taggtaggac
     1801 ttatattaca aatataagtg aggtcacgga aatggaactt acagtagata aagctaaaaa
     1861 tcccaccaag ataacaaaat ttaccctcaa ccatacatcg catcaggagg gtcattatgc
     1921 caatgagtcg actgtctatg tgtttgatga aaaatctgaa cttgctttca aagatgttcc
     1981 catctccctg ctgacgaaat acaccgagaa ggttgatctt ccggccaagt ggatgaaaag
     2041 tgcctcatac ttctacaaca cgaaaggggg agtcgttttc ataaaaggct cggatttatc
     2101 tccttatctc gtatcagcta ccggaactaa aggtgatgag tcttctaaaa aattgttcgc
     2161 tcaaaacact atcatcatgt tctatgtggt acaaaagaag taactgaaat aagaggaaga
     2221 atctaaacag taatttaaat ggggtcacat cataaatcat tctgacgtga ccccattttt
     2281 atataacctg atttattttt ccggaacgtg ttcgtatttg aataaaattg caaatgcaat
     2341 ggtgattacc agcgcgtatg cggcaaaaat gtaccaggtg tggctccagc cggtcatttt
     2401 agcaataggg tctgtctgtg agtttacaag agcgtttact acggcctgag ctccgagtgt
     2461 tccgattgcc gcaccgatac cgttggtcat aagcatgaag agaccctgtg cgctggaacg
     2521 gatgctgtga tcggtattcc tatccacaaa gagcgatccc gaaatattga agaagtcaaa
     2581 tgcaacacca taaacaagca tagaaaggac aaagagccat actccgttgc ccggattgcc
     2641 gagtccgaaa aatgcaaatc tgagcaccca tgcaaacatt gcgataagca tcaccttctt
     2701 gataccatac ctgcgtagga agaaaggtat caacaggata caaagtgctt cagaaatctg
     2761 tgagagtgaa atcaatatat ttgagtgttt caccccaaag gtgttagcat agatttctat
     2821 accggcaaaa tcgcccagga atgggtttgc aaagctattt gtaatttgca gagccacgcc
     2881 gagcaacata gagaagataa agaagatggc cattctgcta tctttgaaga gcgcaaaagc
     2941 tttaagaccc atagcctcca ccagggactt cttttctcca cctttgttga caggacagtg
     3001 agtgagagtg aaactgtaac cggcaaggat aaggccgagt attcctgaga agacatattg
     3061 gtaagcagag gcctgtatgc ccataaggtc cacgcaccac attgaaacaa taaagccgat
     3121 ggtgccccaa atacggatcg agggaaatac cttgacggta tccataccgg ctttttcaag
     3181 ggcattataa gataccgagt tgatcagagc gattgtcggc atgaagaatg ccacgctaaa
     3241 ggagtataat gtgaagatgg ttccgaactg caccgtttcg gcagtcaatc cgtaccagcc
     3301 ggtagctatc attaaaatgg ctgcaatgcc gtggcagata ccgagaagct tctgtgccgg
     3361 aatccatttg tcagcgataa tgccgattaa tgcgggcata aagatggaaa ct
//
LOCUS       DAOOMC010000193         3276 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdus sp. isolate AMR_MDS_2685
            NODE_35503_length_3276_cov_6.331885, whole genome shotgun sequence.
ACCESSION   DAOOMC010000193 DAOOMC010000000
VERSION     DAOOMC010000193.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864760
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdus sp. (wastewater metagenome)
  ORGANISM  Syntrophorhabdus sp.
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae; Syntrophorhabdus.
REFERENCE   1  (bases 1 to 3276)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3276)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,729
            Genes (coding)                    :: 2,708
            CDSs (with protein)               :: 2,708
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 7 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3276
                     /organism="Syntrophorhabdus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35503_length_3276_cov_6.331885"
                     /isolate="AMR_MDS_2685"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2699756"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(67..612)
                     /locus_tag="PK125_13205"
     CDS             complement(67..612)
                     /locus_tag="PK125_13205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HPB39103.1"
                     /translation="MVRLISPFNNKHPLIHPDAFIDLSARIIGAVIIEERVSIWPMVV
                     LRADSESICIKKGSAVLDLSLVEAPAKYPVTIEEETLISHRVVIHGAHIQKRVLIGIG
                     AIVLDGSVVSTGSIVGAGALVAPGTTIPPNSLVMGMPGKVVRETTEKERYFISQQVHE
                     LFNKSRQYRTKEGRTTKNTLP"
     gene            649..2391
                     /locus_tag="PK125_13210"
     CDS             649..2391
                     /locus_tag="PK125_13210"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF024642.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HPB39104.1"
                     /translation="MTLATLSIIENLNEEQIEIVNRIRGPLLVAAGPGSGKTLAIIRR
                     VAMLIQQGVPAQSIIAVTFTNRAAREMKERLSVLMGDTSEKVFVGTLHLLGLRIIQEN
                     TTNRFLIYNRSEQIGLLMSLLQISKTKAMDLADQISYTKNLNIGPSGQLRGPFERYQA
                     AMMEQRALDFDDLILKPLELLDNPDLLQKYRDNIHHIIVDEYQDINTAQYRLLRLLAG
                     NNATVCAVGDPDQAIYAFRGADVENFLNFGKDFVGAAEVTLSLNYRCSGSIVRASDGV
                     IIHNTRRIKKEVKAVKGDGPMIVIASVPDEVAEGEFIAREIDARLGGLRHNSLPDTGN
                     QKEYPGPPFCFSDFAVIFRTNNQAEAIEEKLAAFGIPCRVLGGGGAENRREVTCNMGS
                     LLKQVFTSEEMRDQLFAIRISDLLDILTKNGRCNIDQITNTTNHIRLIFGDAWMEMTA
                     GDMVNELNLFTDQDVFDPRAEAVNLMTLHMAKGLEFRSIFISGVEDGLIPYGAGNGSV
                     DIEEERRLFYVGMTRAKEELFLIYKRTGLVYGKKIKRSPSPFLSHIPEACVERITISD
                     RPRKSRSKEQMKLF"
     gene            complement(2462..3151)
                     /locus_tag="PK125_13215"
     CDS             complement(2462..3151)
                     /locus_tag="PK125_13215"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HPB39105.1"
                     /translation="MKPKRIEDGVFLVGSSNISDSRDCMVYLLDLGELIMIDTGAGPG
                     VSKIISNIRYFGFDPAKLSTIILTHCHIDHVGGAHEFKKRFDVRLVMHNSDVDVVERG
                     DTKMTAAHWYGVPFAPLKIDKRLLQEEERLSFRQHDLICFHTPGHTPGSISVYINMSG
                     KRILFGQDIHGPFLPEFGSDIVLWQASMKKLLSLKADVLCEGHFGVFRPNDKVTEYIE
                     RYLEEYGDQSV"
BASE COUNT          978 a          690 c          750 g          858 t
ORIGIN      
        1 ctgcttatcc aaaatgaaaa ctgaactcag gagattggcg ataggaagtg tcaaatctgg
       61 ctacatctac gggagcgtgt tttttgttgt tcggccctct tttgtcctat actgacggga
      121 cttattgaag agctcgtgga cttgttgaga gataaaatac cgctcctttt ctgttgtctc
      181 ccgcaccacc ttacctggca tacccattac gagtgaattc ggtggaatag ttgtaccagg
      241 cgcaaccaag gcgcctgccc caacaataga tcctgttgaa acgaccgagc catcaagaac
      301 aattgcacca atgccaataa gcaccctttt ctggatatgt gcaccatgaa tcacaaccct
      361 gtggctgata agggtttcct cttcaatagt aacgggatat tttgcagggg cctcaaccag
      421 ggagaggtcc agtacagcag accctttctt aatgcaaata gactcgctgt cagccctgag
      481 aaccaccatg ggccagatgc tcacccgttc ttcaatgatg acagcgccaa taatgcgcgc
      541 agaaaggtca ataaatgcat caggatggat taacggatgt ttattgttaa aaggtgaaat
      601 aagccttacc ataatcaatt atcactatgt tatacttacc acagcgccat gaccctggca
      661 acattatcta taatagaaaa cctcaacgaa gaacaaatag agatagtaaa taggatcagg
      721 ggccctttgt tggtggcggc tggaccagga tccgggaaaa cgcttgccat tatcagaaga
      781 gtggcaatgt tgatacaaca aggtgtgcca gctcaaagca ttattgccgt tactttcaca
      841 aacagggcgg caagggaaat gaaagagagg ctatccgttc ttatgggtga cacatcagag
      901 aaagtgtttg tgggcacact ccatctcctt ggattaagaa ttattcaaga aaatacaaca
      961 aacagatttc taatttataa tcgttcagaa cagataggcc ttttgatgtc tttgcttcaa
     1021 atctccaaga caaaggcgat ggaccttgct gatcaaatct cctacacaaa aaaccttaat
     1081 attggccctt cagggcaact ccgagggcct tttgaacgat accaggcagc aatgatggaa
     1141 caaagagcac ttgactttga tgatttgatt ttaaagcccc ttgagctgct tgataatccc
     1201 gatctgctcc aaaaatacag ggacaatatt catcacatca ttgttgatga atatcaggat
     1261 ataaatactg cccagtacag gctattgagg ctgctcgcag gaaacaatgc aacggtatgt
     1321 gcagtgggcg accctgacca ggcaatctat gccttcagag gagcagatgt ggaaaatttc
     1381 ttaaattttg gaaaagattt tgttggtgcg gcagaggtga cgcttagtct caattaccgt
     1441 tgttcaggaa gcattgtacg agcctctgat ggggtgataa tacacaacac aaggcggata
     1501 aaaaaagaag ttaaagccgt taaaggcgat ggtccaatga tcgttattgc atctgtaccg
     1561 gatgaggtgg cagagggtga gtttattgcg cgggaaatag atgcacgatt gggaggttta
     1621 agacacaata gcttacccga tacgggaaat caaaaagaat atcccggacc tcctttttgt
     1681 ttttcagatt ttgctgtgat ttttaggacg aacaaccagg cagaagcaat agaggaaaag
     1741 cttgcggcat tcggtatacc gtgcagggtg ttgggaggag ggggcgcaga aaatcgcaga
     1801 gaagtgacat gcaatatggg gtccttgctg aagcaggttt ttacctctga agaaatgaga
     1861 gaccaactct ttgcaataag aatatcagat ttacttgata tcctcacgaa aaatggtcgc
     1921 tgcaacattg atcagataac aaacacaaca aaccatatca ggcttatatt tggtgatgct
     1981 tggatggaga tgacggcagg cgatatggtt aatgagctga acctttttac tgatcaggat
     2041 gtttttgatc cacgcgctga agccgtgaac cttatgacgc ttcatatggc aaagggcctt
     2101 gaattcagga gcatatttat ttctggggtt gaagatggtc ttatacctta cggggcagga
     2161 aatggctcag tagacataga agaagagcgg aggcttttct acgtggggat gacgagggca
     2221 aaagaagagc tgtttcttat ttacaagaga acaggcctcg tttacggaaa gaaaataaaa
     2281 cgctccccat caccatttct ttctcatatt cccgaggcgt gtgtcgagag aatcactatt
     2341 tctgataggc caagaaaatc acggtcaaaa gagcaaatga aactgtttta ggcccccttt
     2401 taaagcagca atctggcaga atgcagcttt tttgacactc ttttcaaggg ccactgtatc
     2461 tctaaacgga ctgatcccca tattcctcga ggtatcgttc aatatattct gtaactttat
     2521 cgttgggcct gaatacacca aaatgaccct cacaaaggac gtctgccttc aaagaaagca
     2581 gctttttcat tgacgcttgc cagagcacaa tatcagaacc gaattcaggc agaaacggac
     2641 cgtgaatatc ttggccgaac aaaattcgct tgccagacat gttgatatag actgaaattg
     2701 agccaggcgt gtggcctggg gtgtgaaaac aaataagatc gtgttgcctg aaagaaagcc
     2761 gctcttcttc ctgcaggagt cttttgtcaa tttttaaagg ggcaaaaggc acaccatacc
     2821 aatgtgcagc ggtcatcttt gtgtcgcccc gttcaaccac atctacgtca ctgttatgca
     2881 tgacaagcct gacatcaaaa cgctttttga attcatgtgc accacctaca tgatcgatat
     2941 gacaatgggt aaggataatt gttgaaagct ttgcgggatc aaaaccaaaa taccgtatgt
     3001 tagaaattat tttactgact cccggccccg caccagtatc aatcataatg agctctccca
     3061 ggtcaaggag gtaaaccatg caatcacgag aatctgatat gtttgagcta cctacgagaa
     3121 agactccatc ttcaatgcgt ttgggcttca tcctgcacct tgtataagga tattatcata
     3181 atttattgtc ttggtcgacg taaagctgac tccgttttta aacgaccttg tttgcataaa
     3241 cgttttggca tcgttgcaaa cgagaatttt tgatgg
//
LOCUS       DAOOMC010000194         3216 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdus sp. isolate AMR_MDS_2685
            NODE_36419_length_3216_cov_5.899399, whole genome shotgun sequence.
ACCESSION   DAOOMC010000194 DAOOMC010000000
VERSION     DAOOMC010000194.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864760
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdus sp. (wastewater metagenome)
  ORGANISM  Syntrophorhabdus sp.
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae; Syntrophorhabdus.
REFERENCE   1  (bases 1 to 3216)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3216)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,729
            Genes (coding)                    :: 2,708
            CDSs (with protein)               :: 2,708
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 7 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3216
                     /organism="Syntrophorhabdus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36419_length_3216_cov_5.899399"
                     /isolate="AMR_MDS_2685"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2699756"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(171..1487)
                     /locus_tag="PK125_13220"
                     /pseudo
     CDS             complement(171..1487)
                     /locus_tag="PK125_13220"
                     /inference="COORDINATES: protein motif:HMM:NF013319.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
     gene            complement(1675..2097)
                     /locus_tag="PK125_13225"
     CDS             complement(1675..2097)
                     /locus_tag="PK125_13225"
                     /inference="COORDINATES: protein motif:HMM:NF014058.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="archease"
                     /protein_id="HPB39106.1"
                     /translation="MASVYGTGFRIVEHEADTGLEIYGRNLFELYNHGGTALFSLITD
                     PATIELHVTKEITLDIEEESLVVFLNELLYLWDAEKFLPGRFSVSKERGRLVVHATGE
                     IFDPGRHPVKKEVKAATYHKFSIQQQGDLLKATIFLDI"
     gene            2318..>3216
                     /locus_tag="PK125_13230"
     CDS             2318..>3216
                     /locus_tag="PK125_13230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB39107.1"
                     /translation="MKKFIVIAIAVLLAIPAVSFAGSATSRWDLTLGGYIEFDLGWAD
                     RGVNADYAIAPRKSYGIWENREAEYASEFAAGGAGRLWWIIKGPDGWGGKTSAYVETD
                     FRGQAGDYGEMNLRHAWMKIEWANDSLQIGRQWQRWGAHFPFGGWLLMFSGLGPSLKG
                     QRNEAIVWDHKFTKDWGFYLGVYHNSANDMGARALDNFANSPFPFIEGGIRYASDSCG
                     RIGNQIMMFEAGGFLGWERNIWAYTPAIGGRAYDDDRATAWGAALKYYVPIIPERKGN
                     KAGAFGVGGNAFIGQNLGWYGGIG"
BASE COUNT          804 a          783 c          749 g          880 t
ORIGIN      
        1 cgcaatgcca agagccgctg tggtcttgcc tttgccgttc ccggtaaata caataatgat
       61 tccttttttc tccatgtaca ccccccttta aataccgtga tccgtgaaca gtgacgcgtg
      121 atgggataaa aaaacggatc accgatcacg cgtaacgcgt tacggttttc tcatcctttt
      181 atcgacccta aaggaacaat ttttgcgaca agacgtgata tgccggcatt gtgcaccacg
      241 ttgacgacct tcgacacatc cttgtatgcc tcgggtgctt cttctgccag tgtctccttg
      301 cttgcacccc tgacaaagac tcccctggct tccatttccc tgtagatcga acgacctttt
      361 gctgctttga tcgcctgaga tctgctcata acccttcctg cgccatgaca ggtgctgccg
      421 aaggtttcct ccattgcttt ttcggttccg cagagcacat aagaggctct gcccatatct
      481 ccgggaataa ggactggctg gccaactgat ttataccgtg atggcactgc cggattaccg
      541 ggtgcaaacg acctggtagc gccttttcgg tgaacacaga cttcggctgt cttcttgttc
      601 actgtatgtt tttcgatctt tgcaatgtta tggcatacgt cgtagacgag ggagagcccg
      661 agtttgccct ggctttgtct gaagacgcct tcaaaggttt cccgaaccca atgagttatc
      721 atttgcctgt ttgcaaaggc ataatttgca gcgcctgcca tggcggaaag atatttttga
      781 ccctcatctg cctgaatagg agcacagcac aattgcctgt ccgggagaaa aatgccgtac
      841 tttcctgatg ctttaatcat ttcccggata ctgtcgtcac agatctgata tccaaggccc
      901 ctggagccgg tatggatcat aatggtgacc tgcccctcaa acaaaccgaa attttctgca
      961 gcatgagcat catatatttc ctggatgaag cctacttcca cgaaatgatt gccgcttccc
     1021 aaggttccaa gctggtcctt ccccctctca tatgccctgt cagatacagc atcggggtct
     1081 gcattgtcaa ggcagccccc atcttccatg aacaggaggt cttcctgtga tccataccca
     1141 ttctcaacag cccatcttgc acctttcttc agaacctttt ttaactcatc tcttgtgagc
     1201 tttaaatctt ttctatgtga tccgagccca ctcggaatgt ttatgtaaag gctatcgatg
     1261 atttccttta tctggttttt tgcttcattc acgctcaaca cggagcgtat caatcggacc
     1321 cctcagttta tatcgtagcc gacccccccg ggcgaaacca ctccttcgtt gagatcaaaa
     1381 gcagcgaccc ctcctatagg gaaaccatag ccccagtgaa tatcaggcat ggccagggag
     1441 gcatgaacaa ttccggggag tgtggcaaca ttctcaacct gcttcatact ctcatctgtg
     1501 ccgagtatct tcaggagatc ctcatcaata tagacaatcc cgggcactct catggcatcg
     1561 tgtttttcaa tcatgaaccg aaaatcgtcc atttttctga gatgtgtact tttttgatcc
     1621 ttcataacaa cttcttccta tatagtatac attattttga gaatccacat agccctatat
     1681 atccagaaaa atagtggctt tcagcagatc cccctgttgt tgtatggaga atttgtggta
     1741 tgtggcagct tttacctctt ttttaaccgg atgcctgcca ggatcaaata tttcacctgt
     1801 ggcatgaaca acaagccttc ctctttcttt tgaaacggag aatctgcccg ggagaaattt
     1861 ttcagcatcc cacaggtaga gcagttcgtt cagaaagact acaagcgact cctcctcgat
     1921 gtccaaagta atttcctttg taacgtgcag ctcaatagta gcaggatctg taatgaggga
     1981 aaaaagggct gtgcctccat ggttgtaaag ctcgaaaaga tttcttccat atatttcgag
     2041 gcctgtatca gcctcatgtt cgactatcct gaagccagtt ccataaacgc tggccatttt
     2101 atcatcattg tgaataacta ccatattata tagtatagaa tacttttttt aaaatgtgcg
     2161 cgatctcaga ctgtaacgct cagattaaaa aaaattaaaa atttttaatt tttttcttga
     2221 cagaccgata ttctattgat ataagaatta aaaaatttta ataaaaggag gtggtatggg
     2281 ctttttgaca cacaaaaata atgataagga ggtaagaatg aagaagttta tagttattgc
     2341 aattgcagta cttcttgcaa ttcctgcagt aagcttcgca gggagcgcca caagcagatg
     2401 ggatctgacg cttggcggct acattgaatt tgacctgggc tgggccgaca ggggagtgaa
     2461 cgctgactac gcaatagccc ccagaaaatc ttacggcatt tgggagaaca gagaggctga
     2521 atatgccagt gaatttgcag ccggcggtgc aggcagactc tggtggatta tcaagggccc
     2581 tgatggatgg ggcggtaaga caagcgccta tgtagaaact gatttcaggg gccaggcagg
     2641 tgactatggc gaaatgaacc tcaggcacgc atggatgaag atcgagtggg caaacgacag
     2701 cctgcagatc ggacgtcagt ggcagcgctg gggcgctcac tttcctttcg gcggctggtt
     2761 gctcatgttc tccggccttg gaccgtctct gaaaggccag agaaacgagg ccatcgtgtg
     2821 ggatcacaag tttaccaaag actggggctt ctatctgggc gtataccaca actcggccaa
     2881 cgatatggga gcacgtgcgc ttgacaactt cgccaacagc ccgtttccgt ttatcgaagg
     2941 cggtatcagg tatgcatccg atagctgcgg aaggatcgga aaccagatca tgatgttcga
     3001 ggcaggcggt ttcttaggat gggaacggaa tatttgggca tatacgccag ctattggcgg
     3061 cagggcctat gacgatgaca gggcgactgc atggggtgca gcattaaagt attatgtccc
     3121 cattatccct gagaggaaag gcaacaaggc cggtgccttc ggcgttggcg gtaatgcttt
     3181 tataggccag aaccttggat ggtacggtgg tatcgg
//
LOCUS       DAOOMC010000195         3195 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdus sp. isolate AMR_MDS_2685
            NODE_36743_length_3195_cov_6.885350, whole genome shotgun sequence.
ACCESSION   DAOOMC010000195 DAOOMC010000000
VERSION     DAOOMC010000195.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864760
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdus sp. (wastewater metagenome)
  ORGANISM  Syntrophorhabdus sp.
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae; Syntrophorhabdus.
REFERENCE   1  (bases 1 to 3195)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3195)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,729
            Genes (coding)                    :: 2,708
            CDSs (with protein)               :: 2,708
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 7 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3195
                     /organism="Syntrophorhabdus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36743_length_3195_cov_6.885350"
                     /isolate="AMR_MDS_2685"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2699756"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(160..918)
                     /locus_tag="PK125_13235"
     CDS             complement(160..918)
                     /locus_tag="PK125_13235"
                     /inference="COORDINATES: protein motif:HMM:NF013998.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HPB39108.1"
                     /translation="MGISGFFGRNNLSKIELKVELAPEIFATAKTPVSVSVTNKRRFL
                     PIFLLRINIQNRELFFPFIEAGKSASKHVDMTFEQRGRYTLGLISLNSAFPFNFFVRY
                     RWYNEESSFVVFPRLQQCKLFSFYEKERHSKGEYSSDTAGFDSDIISIREYVPGDPIK
                     YINWKATARTGELKTKELSSLTFQPVIIDFSTVDIENTEEKISCVTYAIVQLIKKNIP
                     IGLRLGSIFFSPSASCSHRTNLLTELALYGTHES"
     gene            complement(1035..1988)
                     /locus_tag="PK125_13240"
     CDS             complement(1035..1988)
                     /locus_tag="PK125_13240"
                     /inference="COORDINATES: protein motif:HMM:NF019346.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HPB39109.1"
                     /translation="MQQQSLANEEIRDKIDLITDSLGKHLQGKETSLRLSLISFFSGG
                     HLLIEDLPGLGKTTLAIGIARALGLSFGRIQCTSDLLPTDITGLSIYNKATGEFKFHR
                     GPIFNNIVLCDEINRATPKTQSALLESMAEKQVTIEGKTYRLPDIFCVIATQNPVEHY
                     GTFPLPESQLDRFMMKISIGYPSHKAEKMILRIGSTQEILHTIEPVMDPAEVKLVQEE
                     IEKNIYVSDRILDYVLAIVHGTRQNDFLTAGLSTRGALTIINTAKTSAYFHGRDFVIP
                     EDIKELAEFTIPHRVIFKEEYENTDKREIIKSFIEEIPTPA"
     gene            2243..3160
                     /locus_tag="PK125_13245"
     CDS             2243..3160
                     /locus_tag="PK125_13245"
                     /inference="COORDINATES: protein motif:HMM:NF021445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerol lysyltransferase
                     domain-containing protein"
                     /protein_id="HPB39110.1"
                     /translation="MPDLNMPQFPMFVPLELKDQEVFQTFFSAYRPEISELTFTNLFI
                     WRSHYKFQWSIYRDWLVIISVEGEYGTFAIEPIGPSPRYEITRLVLEWMRDEKNVKNS
                     RIERADNRIVEEVRGINGISVEPTRDHFDYVYLRDDLVKLGGNRYRSKRNHINQLVRA
                     YSYRYEQLAPDHINDCIAVQEKWCLQRRCEDDMDLLGEWDAVKEILRCYMNLNVQGAV
                     ITIENKVVAFTIGQMLNENTAVIHIEKADPEIPGLYPVINQQFCENNWQGVRYINREQ
                     DLGIPGLREAKLSYYPDHMVNKFRIMLNQ"
BASE COUNT          922 a          632 c          737 g          904 t
ORIGIN      
        1 cgaaaactcc tcctgtaatc gaaatagagg gagatagcaa aaagaaggca gaatacaatt
       61 gaataaagag aaccaatgtc ctttacaata gaaagaaaga ccatggcacc tacgaggtag
      121 cttatgaaaa taacaatcgt ttcaatcctg aaattgcggt tatgattcat gggtcccata
      181 gagtgcgagt tcggtcaaaa ggtttgttct gtggctgcag gacgcagagg ggctgaaaaa
      241 gatgctgccc agtcgtaacc caatgggaat gtttttcttt attagttgaa caattgcata
      301 ggtgacacag gaaatttttt cctcagtgtt ttcaatatca actgttgaaa aatctatgat
      361 aacgggctgg aatgtgaggg atgaaagctc ctttgtcttg agttctcctg tcctggcagt
      421 tgccttccaa ttaatatact tgatagggtc tccaggaacg tactctctga tggatataat
      481 gtctgaatca aatccagccg tgtccgaaga atactcgccc tttgaatgcc gttctttctc
      541 gtaaaaagaa aaaagcttac attgctggag tctgggaaac acaacgaaac tggattcttc
      601 gttgtaccac ctgtaacgca caaaaaaatt gaaggggaaa gctgaattta atgaaataag
      661 ccccagggtg tatctacctc tttgttcaaa ggtcatgtca acatgctttg atgcagattt
      721 ccctgcttcg atgaatggaa agaaaagctc ccgattctga atattgatcc tcagtaaaaa
      781 tatgggaaga aatcttcgtt tatttgtaac agaaacgctg acaggtgttt tggcagttgc
      841 aaatatttca ggggccaact caaccttgag ctctattttt gagaggttat tccgcccgaa
      901 gaacccggaa atacccataa aacccaggag agcggagaca acaaggtaga taaggttgtt
      961 cgcagtgttt actgcggcga ttcccagaaa aatggtaaga aggatataaa gaaagcctgc
     1021 acgggtaatc tttattacgc gggggttggg atttcctcga tgaaagattt tatgatctcc
     1081 ctcttgtctg tattctcgta ttcctcctta aaaataaccc tgtggggtat ggtgaattct
     1141 gcaagttcct tgatatcctc agggatcaca aaatcacgtc catggaagta ggcactggtt
     1201 tttgctgtgt tgatgatggt aagggcaccc cgtgttgaga ggccggcagt aagaaagtca
     1261 ttctgtcttg tgccatgaac tatggcgaga acatagtcca gtatcctgtc agatacatag
     1321 atgttttttt caatttcttc ctgaacgagt ttgacctctg caggatccat taccggctca
     1381 atagtatgga ggatttcctg tgtgcttccg attctcagaa tcattttctc agccttgtgg
     1441 gacgggtagc cgatgctaat tttcatcata aaccggtcca attgtgactc aggcagcgga
     1501 aaagtaccgt aatgttcaac aggattttga gtagctataa cacagaatat atcgggaagc
     1561 ctgtacgttt ttccttctat agtcacctgt ttttcagcca tagattcaag aagggcgctc
     1621 tgtgttttag gtgttgcccg attaatctca tcacaaagga cgatgttgtt aaagatgggc
     1681 ccccggtgaa acttgaactc cccagttgcc ttgttataaa tcgaaagtcc tgttatatct
     1741 gtggggagaa ggtcgctggt gcattgtatc cttccaaaac tcaaacccaa tgctctggca
     1801 atgcctatgg cgagggtagt tttgccaagg ccggggagat cttcgataag tagatgcccc
     1861 ccggaaaaaa aagagatgag tgaaagcctc agggatgtct cttttccttg caggtgcttt
     1921 ccgagagagt cagtgatgag atctatctta tctctaattt cttcatttgc cagactttgt
     1981 tgttgcacag atcaacctgt tgaaagttct ttatcaattt tatcgtattt taacatgatt
     2041 tacctaagaa gggcagtgat gaatatcctg tcgatcatcc ttttagcaat aatttctgcc
     2101 atccttcggg gtatggaaag caactgaact gtaagacgcc tgccagaatc gatccgttta
     2161 tgtaattatg ccggaaatca aatagaccaa caacgatatt accatagttg atttgaacac
     2221 ctgagagttg ttatattcac taatgcctga cctaaatatg ccacagtttc caatgtttgt
     2281 gcctttggag ttgaaagatc aagaggtctt tcagactttt ttctcggcat acagacccga
     2341 gatatcggag ttgaccttca cgaacctttt tatatggcgc tctcattaca aattccaatg
     2401 gtcaatatac agggactggc tggtgattat cagcgttgag ggagaatatg gcacctttgc
     2461 cattgaacca atcggtcctt cgccccgcta tgaaatcacc cgtttggttc tggaatggat
     2521 gagagatgag aaaaatgtaa agaattcgag gatagagcgg gccgacaaca ggattgtcga
     2581 agaagtcaga ggtataaatg gtatctctgt cgagccaaca cgcgatcatt tcgactacgt
     2641 ttacctcagg gatgatctgg ttaaactcgg gggtaacagg taccgttcta aaagaaatca
     2701 cattaaccag cttgtccggg cttattcata ccggtatgaa caacttgctc ctgaccatat
     2761 caatgactgt attgcagttc aggagaaatg gtgcctccag cgccgttgtg aggatgacat
     2821 ggatctcctt ggggaatggg atgctgtaaa ggaaatcctg agatgttaca tgaaccttaa
     2881 tgtgcagggt gctgtaataa cgatagaaaa caaggttgtg gcttttacga ttggacagat
     2941 gttaaacgaa aatactgctg tgatccatat cgaaaaagct gatccggaga ttccagggct
     3001 ttacccggtc attaatcaac agttttgcga gaataactgg caaggtgttc gctatattaa
     3061 ccgggaacag gatctgggta ttcctgggtt gagagaggcc aagctctcct attacccgga
     3121 ccatatggta aataaattcc gcatcatgtt aaaccagtga aaagttagaa gtgaaaagta
     3181 aaaagtaaaa cccat
//
LOCUS       DAOOMC010000196         3190 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdus sp. isolate AMR_MDS_2685
            NODE_36838_length_3190_cov_6.998405, whole genome shotgun sequence.
ACCESSION   DAOOMC010000196 DAOOMC010000000
VERSION     DAOOMC010000196.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864760
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdus sp. (wastewater metagenome)
  ORGANISM  Syntrophorhabdus sp.
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae; Syntrophorhabdus.
REFERENCE   1  (bases 1 to 3190)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3190)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,729
            Genes (coding)                    :: 2,708
            CDSs (with protein)               :: 2,708
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 7 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3190
                     /organism="Syntrophorhabdus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36838_length_3190_cov_6.998405"
                     /isolate="AMR_MDS_2685"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2699756"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..243
                     /locus_tag="PK125_13250"
     CDS             <1..243
                     /locus_tag="PK125_13250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB39111.1"
                     /translation="NIRMTQKFQEVQGTLPGNNNEPFLTCSLKGAAIRCTTGSFSIEG
                     RVSGDVIKGAVNYGVRGPTRTVKATRNPSTKVSIAE"
     gene            393..1145
                     /locus_tag="PK125_13255"
     CDS             393..1145
                     /locus_tag="PK125_13255"
                     /inference="COORDINATES: protein motif:HMM:NF013782.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase"
                     /protein_id="HPB39112.1"
                     /translation="MCLIPAVWKEKRVIESFDVDILGRLHPQTLFSFLLNAAWNHACK
                     GTYGFTELTAHDLYWVLVKAQILIKRLPNWREQITIETWGKRKVRLYALRDFIITSEE
                     GEKLISATSSWMILDRKNGKPQRFDEKSESLPWNPQKNEIETSLEKVPQLNYGKELRK
                     FRIHFSDIDVNRHVNSAKYVEWIINSHSQKHLETNNIGSIEISFLSEASSDDVVAVYS
                     EVSNGFRLYSVKNYVDEKELCRARIKWQPCET"
     gene            1266..3089
                     /locus_tag="PK125_13260"
     CDS             1266..3089
                     /locus_tag="PK125_13260"
                     /inference="COORDINATES: protein motif:HMM:TIGR01813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavocytochrome c"
                     /protein_id="HPB39113.1"
                     /translation="MNNWIPRQKYSALLPFVVLLGMLVISCTVPQAQHSRQEALEPTK
                     AALVSPGGSLVAIHEAKAVDCAACHGKNQVVDDNETIVNTKCIECHGPLPEMAKKGKG
                     HINAHKSHLGQINCTVCHHGHVASRPYCLGCHSFNMPIPGSGGPLAVKKEPVRSDKKV
                     DSTDIVIVGAGAAGFTAAITAHDAGAKVILVEKNPITGGNSMLSAGGMNAAATQMQTK
                     KNIKDSPDIMFKDTMTGGKNTNNPALVKILAEKSAESIDWLTSIGADVSDVGRLAGAT
                     VNRAHRPTGGAAIGAHLVDVLRKNAADRKIDVRLNSRVIQVLRDNNGRATGIQVEGKH
                     RGLYVINAKAVIIASGGFSANAVMVESYRPEYKGMTTSNQPGATGDGMRFGETAGGKL
                     IGMKDIQIHPTVAYGSRILITEAVRGNGAILVNKEGKRFVNELTTRDAASAAILKQQD
                     KMAYLIIDENIRKSLRQIEGYFHLELVKEGATLRDLAAAIGAHPDTLEATVAKYNTAV
                     ETKNDIEFKRADMPRVIKTPKFYAIGVQPGIHYTMGGLEIDEQTRVIDNNGKPIPGLY
                     AAGEVTGGVHGANRLGGNSISETITFGRIAGTTTAKVVLKK"
BASE COUNT          995 a          690 c          728 g          777 t
ORIGIN      
        1 aacatcagaa tgactcagaa atttcaggag gtacaaggca cattgcccgg taataacaac
       61 gagcctttcc tgacctgctc cctaaaaggc gcagccatcc gttgcactac aggttctttt
      121 tctattgaag gacgcgtatc aggcgatgtt atcaaaggtg cggttaacta tggtgttcga
      181 ggccctacaa gaactgtaaa agcaactcga aacccatcaa ccaaagtctc aatagctgaa
      241 taaaaaagga atattttacg tgtcaacaaa ttcctgtttg tgctacctgt tggtcgtgac
      301 actggtaagc tataagacca accgcccgtg gtcagatact ttggcactca tttcgtcctt
      361 gtcttatcac ctgacaaatc gtatcatttt aaatgtgtct aatcccagcc gtttggaaag
      421 aaaaaagagt cattgagtca tttgatgtag atatcctcgg ccgtctacac cctcaaacct
      481 tattttcctt tctcttgaac gcagcctgga atcatgcctg caaaggtaca tatggattca
      541 cggaattgac tgcccacgat ttgtactggg tactcgtaaa ggcacaaata ctcattaaac
      601 gattgccaaa ttggcgcgaa cagataacaa ttgaaacatg gggtaagagg aaagtgaggc
      661 tttatgcact ccgagacttt attatcacat cagaagaagg agaaaaactt atatcggcca
      721 cttcctcttg gatgattctg gacaggaaaa acggcaaacc tcagcgtttt gatgagaaat
      781 cagaaagtct cccgtggaac ccccaaaaga atgagataga aaccagtctt gaaaaggttc
      841 cacagttaaa ttacggtaaa gaactcagaa aatttcgaat tcatttttct gacattgatg
      901 ttaaccggca tgtgaattcc gccaagtacg tagagtggat catcaatagc cactcacaaa
      961 aacatcttga gacaaataat atcggatcta ttgaaataag tttcctttca gaggcaagtt
     1021 cggatgatgt ggtggcagtt tattcagaag tatcaaatgg attcagactg tacagcgtga
     1081 aaaattacgt agatgagaaa gaactttgca gggcgcggat aaaatggcaa ccctgtgaaa
     1141 catagaattt gaataaacaa tatacatgaa aatgaatata cgatattcca tttcagttgc
     1201 ttttccaaag tttagacgat aaaattcctt gtcttcgttg acaaataaat tcaggagata
     1261 agcagatgaa taattggatt ccccgtcaaa aatattcagc cttattgcct tttgtagttc
     1321 tgttgggcat gctggtcata tcttgtaccg tcccacaagc acagcactcg cgccaagaag
     1381 cacttgagcc aacaaaagca gcgttggttt ctcccggcgg cagcctcgtt gctattcacg
     1441 aagccaaagc tgttgattgt gctgcctgtc atggtaaaaa ccaagtggta gatgacaatg
     1501 aaacgattgt caataccaag tgtatagagt gtcatggtcc gcttccagag atggcaaaaa
     1561 aaggcaaagg tcacataaac gctcacaaat cccatctcgg acagataaac tgcacggttt
     1621 gtcaccacgg acacgtggct tcaagaccat actgcctggg ttgtcactca tttaacatgc
     1681 ctatacccgg aagtggcgga cctctggcgg taaagaaaga gcctgttaga agcgacaaaa
     1741 aagtggactc cactgacata gttatcgttg gcgcaggagc cgcaggtttt acagcagcca
     1801 taactgccca tgacgctggg gcaaaggtca tccttgtcga gaagaaccct attacaggcg
     1861 gcaacagcat gctttccgca ggaggcatga atgctgcagc cacgcaaatg caaacgaaga
     1921 aaaacattaa agacagccct gatatcatgt ttaaggacac aatgacaggc ggtaaaaata
     1981 cgaacaaccc tgccctagtc aaaatacttg cggagaaatc agccgaatcc attgactggt
     2041 taacatctat cggagcagat gtaagcgatg tgggccgatt ggccggagca actgtcaata
     2101 gagcccaccg tcctactgga ggagcagcaa taggcgctca tcttgtagat gttcttcgta
     2161 aaaatgcggc tgacaggaag attgatgtgc gattgaacag cagggttatc caggtactcc
     2221 gagataacaa tggacgcgcc acaggtatcc aggtggaagg taagcaccgt ggactgtatg
     2281 tgatcaatgc aaaagctgtt atcattgctt caggtggctt ttctgcaaat gcggtcatgg
     2341 tggaatcata tcgacctgaa tataaaggta tgactacttc gaatcaacca ggagcaacgg
     2401 gagacggtat gcgctttggt gaaaccgcag gggggaagct cattggcatg aaagatattc
     2461 agatacaccc cactgttgca tatggaagca ggatactcat taccgaagca gtacgtggaa
     2521 atggtgcaat ccttgttaac aaggaaggca agagatttgt aaatgagttg acaaccaggg
     2581 acgctgcatc agctgcaata ctaaaacaac aggacaaaat ggcttatttg atcattgatg
     2641 aaaatatccg caaaagcctt cggcagattg aaggctactt tcatctggaa ctagtaaaag
     2701 aaggtgccac attgagggat cttgctgctg ctattggtgc acatcctgac acattggaag
     2761 caacagttgc aaagtataac acagcagttg aaaccaagaa cgatattgaa tttaaacgag
     2821 ctgacatgcc acgtgttatt aagactccaa agttctatgc catcggcgta caaccaggca
     2881 ttcactacac aatgggcggg cttgaaattg acgaacaaac cagggtaatt gacaacaatg
     2941 gtaaaccaat tcctggtctt tatgctgccg gtgaggtgac aggtggggtc cacggggcaa
     3001 atcgcctggg cggcaactct atttcagaaa cgattacctt cggacgaata gcaggtacaa
     3061 caacggcaaa agttgttcta aagaagtaga tcaagagcca aggggtagaa ctaatctgcc
     3121 ccttggctac cgcccttcat gggcattaac ctcgcgtccc ttttcattgt caattttttc
     3181 atctcctttg
//
LOCUS       DAOOMC010000197         3181 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdus sp. isolate AMR_MDS_2685
            NODE_36967_length_3181_cov_5.527511, whole genome shotgun sequence.
ACCESSION   DAOOMC010000197 DAOOMC010000000
VERSION     DAOOMC010000197.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864760
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdus sp. (wastewater metagenome)
  ORGANISM  Syntrophorhabdus sp.
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae; Syntrophorhabdus.
REFERENCE   1  (bases 1 to 3181)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3181)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,729
            Genes (coding)                    :: 2,708
            CDSs (with protein)               :: 2,708
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 7 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3181
                     /organism="Syntrophorhabdus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36967_length_3181_cov_5.527511"
                     /isolate="AMR_MDS_2685"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2699756"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            363..965
                     /gene="smbP"
                     /locus_tag="PK125_13265"
     CDS             363..965
                     /gene="smbP"
                     /locus_tag="PK125_13265"
                     /inference="COORDINATES: protein motif:HMM:NF028095.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small metal-binding protein SmbP"
                     /protein_id="HPB39114.1"
                     /translation="MKRGTCYVLFFVFAVISLMLLVGCSKPPTEALQKAEQALEEAKQ
                     KGANLYVEDLFKKAEEGLKKAKDQVAAKQYKEAAQTLLEVIPAAEQAVAGIEAGKAKM
                     KEEAEKFVGEAQKGLDELKTDVATAIKNKLPVAKEEIQAAIGKMEVDFAGAKEKLQSG
                     SIREAFDGLKEIIETLKAKKEDIAKLATTPPPAPAEPPKK"
     gene            complement(1200..1325)
                     /locus_tag="PK125_13270"
     CDS             complement(1200..1325)
                     /locus_tag="PK125_13270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB39115.1"
                     /translation="MMGYDNDGDRKLVVRTERKNLCCKVFDAESVAVANTIGKIT"
     gene            1443..2999
                     /locus_tag="PK125_13275"
     CDS             1443..2999
                     /locus_tag="PK125_13275"
                     /inference="COORDINATES: protein motif:HMM:NF024991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HPB39116.1"
                     /translation="MNKEFRTALEIMETSSSHVFITGKAGTGKSTLLDYFRQTTSKDV
                     AVLAPTGVAALNIQGQTIHSFFNFKPNITPEKVKKVSGPDGHIYKEFNTIIIDEISMV
                     RADLLDCVEKFLRLNGPKRRQWFGGIQMIFIGDLYQLPPVVTSAEKEIFSHRYSSPYF
                     FSSQIFREPTFQMEFIELEKVYRQTEPDFIALLNSIRNRSCTDEDMERLNENHRKAND
                     EGDEAFSITLTSTNDLATERNLEKLGALPGKVLEFAGVLNGEFDHTSLPTDETLQVKP
                     GAQVMLVNNDKYGRWVNGTLGKIAGIEKTDNEDIVIIVTLQDGTVVEVAQNTWELFKY
                     EYDRSTKKISTKKTGSFTQYPVRLAWAVTIHKSQGKTFEKVVIDIGRGTFAHGQVYVA
                     LSRCTNFAGITLTKPITKAHIRMDWRVAKFLSSFQYSKAEEKISYEERRRIVEDAIKR
                     DIDLQILYLKPDDRKTQRTIRPIAIEEMEYKGKVFEGLRAYCHLRKTERTFRIDRILE
                     IAYDIGGNHY"
BASE COUNT          991 a          677 c          722 g          791 t
ORIGIN      
        1 gagtcagact gggaaaagaa gactggcggc ccatggaagg aatgggttgg cacttccaac
       61 aaatagcacg gaacatttgt gctgccaagt acgcttacgc caataaatca taccgtgatt
      121 ttgagaggga tcacagggac tccaaatgca tgactgaaaa aggggagtat tagaaaaggg
      181 tgtcccggtt tgcgaagaaa taagaacctg ttttgtgaca tacatgtgat ggggcagtgc
      241 actctggtag tactacctat cgcaaataga ctggaaagta ctgattacca ggtgaaaaag
      301 tgttttgatt ttaacactgt gtggtggtat actgaaaaaa attatttcag gaggtagggc
      361 tcatgaagag gggtacttgt tatgtgttgt tttttgtgtt cgctgttatt tcgcttatgc
      421 ttttagtcgg ttgttctaaa cctcccacag aagcactgca aaaggcagaa caggctctcg
      481 aagaggcaaa acaaaaaggg gcaaaccttt atgtagagga tttgtttaaa aaggccgaag
      541 agggtctgaa gaaggcaaaa gatcaggtgg ctgcaaaaca gtataaagaa gcagcccaaa
      601 cccttttgga agttattcct gcagctgaac aggctgtggc aggcattgaa gctggaaagg
      661 ccaagatgaa ggaagaagca gaaaaatttg tcggggaggc tcagaaaggt cttgatgagc
      721 tgaagacaga tgtagccact gctataaaga ataaactacc agtagctaaa gaggagattc
      781 aagcagctat tggaaaaatg gaagttgatt ttgcaggtgc caaggaaaag cttcagagcg
      841 gaagcattcg agaggccttt gatggtttaa aagagataat tgagacgctg aaggcgaaga
      901 aagaagacat tgccaaactg gccactacac ctcctcctgc tcctgcagag ccacctaaga
      961 agtagcgcat aacatacggg aggcaattag tgcctcccgc tccagccctt ttgtttttgt
     1021 caggccacca tgacaatgat tgttatttag gcttacaggt taacggttgt aagctacacc
     1081 gtaaagaaac tgataaacca catttttaag ctgacctttg aaagtttcaa aaataatata
     1141 tcagccattt caaatacata gggatgcaca ccacggccta ccctttgatc ttacgaagat
     1201 catgttatct tccctatcgt attcgcgaca gcaacgctct ctgcatcgaa cactttgcaa
     1261 caaaggttct tgcgttcggt tctgacaaca agttttctat cgccatcatt gtcgtacccc
     1321 atcattcttc tttacatttc ctcctatcca taatacaacg atattactat gttatgctat
     1381 gaggcctgca gaaaagagag gataagatca taaaatatcg tcaaagaaaa caacctattg
     1441 agattaacaa ggaatttcgc acagcccttg aaattatgga aacttcaagc agtcatgttt
     1501 tcataacagg aaaagcagga acaggaaaat caaccctcct tgactatttc aggcagacca
     1561 ccagcaaaga tgtcgctgtt ctggcgccca caggtgtggc tgccctcaat attcagggcc
     1621 agaccattca ttccttcttc aacttcaagc cgaacataac acccgaaaag gtgaaaaagg
     1681 tttccggtcc agatggccac atttacaaag agttcaatac cattattatt gatgagatct
     1741 ccatggttcg agcagatctt ctcgactgcg tggaaaagtt tctccgcctg aacggaccta
     1801 agcgcaggca gtggtttggc ggcatccaga tgatctttat tggtgacctc tatcagcttc
     1861 caccagtagt tacctctgca gaaaaagaga ttttttccca tcggtactcg tcgccctatt
     1921 tcttttcatc ccagatattc agagaaccga ccttccagat ggagtttatt gagcttgaga
     1981 aggtgtatcg ccagacagag cctgacttca ttgctttgct caattccatc cgtaaccgtt
     2041 cctgcactga tgaagatatg gaacggctga acgagaatca tcgcaaagcc aatgatgaag
     2101 gagatgaagc gttttctatt accctgacga gtacaaatga tctcgctacg gaacgcaatc
     2161 tggaaaagct tggtgccctt ccgggaaagg tattggagtt tgcaggggtc ttgaacggag
     2221 aatttgacca cacctcttta cccacagacg agactcttca ggtaaagcca ggggcacagg
     2281 taatgcttgt caacaacgat aagtacggcc ggtgggtgaa cggaaccctc ggaaagattg
     2341 caggcattga aaagacagat aatgaagaca tagtaatcat tgttacactt caggatggaa
     2401 ctgttgttga ggtagcacaa aacacctggg aactttttaa atacgaatac gacagatcaa
     2461 ccaagaaaat ttcaacgaaa aaaacaggct cctttaccca gtatcctgtg cggcttgcct
     2521 gggccgtaac aatccataag agtcaaggga aaactttcga aaaagtggtg atcgacatag
     2581 gccggggcac ctttgcacat ggacaggttt atgttgcctt aagcagatgt acaaactttg
     2641 ccggcattac ccttacaaaa ccaattacca aggcacatat ccgcatggac tggcgtgttg
     2701 caaagtttct ttcatctttt caatatagca aggctgagga gaaaataagc tacgaagaga
     2761 gacgaagaat agtagaggac gctataaaac gcgacataga ccttcagatt ctttacctca
     2821 aacccgatga tcgcaaaacc cagcgcacca ttcggcccat tgcaatcgaa gaaatggagt
     2881 ataaagggaa ggtcttcgaa ggtctcaggg cctattgcca tctccgcaaa actgaacgga
     2941 cttttcgaat tgaccgtatc ctcgagatag cctacgatat tggcggaaac cactattaaa
     3001 aacaaaaaca ctgctgttcg attaaacatc aaatgaacca aattggggcg taatactaaa
     3061 aaacggggtc ttagttccac agtatttcct caatgatctt gtctttcagt tttcttgctg
     3121 atcctatgtg aactctcacc aattctttga ggtaagcaaa acaaccatcg agagagttgg
     3181 t
//
LOCUS       DAOOMC010000198         3145 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdus sp. isolate AMR_MDS_2685
            NODE_37523_length_3145_cov_5.245955, whole genome shotgun sequence.
ACCESSION   DAOOMC010000198 DAOOMC010000000
VERSION     DAOOMC010000198.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864760
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdus sp. (wastewater metagenome)
  ORGANISM  Syntrophorhabdus sp.
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae; Syntrophorhabdus.
REFERENCE   1  (bases 1 to 3145)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3145)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,729
            Genes (coding)                    :: 2,708
            CDSs (with protein)               :: 2,708
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 7 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3145
                     /organism="Syntrophorhabdus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37523_length_3145_cov_5.245955"
                     /isolate="AMR_MDS_2685"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2699756"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2571)
                     /locus_tag="PK125_13280"
     CDS             complement(<1..2571)
                     /locus_tag="PK125_13280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB39117.1"
                     /translation="MKISFRISIICLMLLVVPILSEGQDMTDYCQLPSSIEAPIDPNL
                     LLVIDVSGSMGWKAYSYGDTDSNSDGYLDGYNATTTYEGYFDPAKFYELNASNVYVET
                     PTGLPCVKECTKWKCQNYNLGDCVKGAQGCGKTKWACCTAWKSSGNCTLTSGNYLNYQ
                     KMTRIDVLRWTLTGGKLQSCANSVETCDPEVYPNSKLSCNNDGCILLSSDGATKVKVP
                     WARITGNSGGLLFQLKNLSPKPIIGAMFYDTNGVTRTVYIGDFTSSSDASYDVTHPYK
                     NVITAINYEAPGGSTPTGPALWDAYNYFSQKSPQYGGPGPQTDVNTKWKNPMNRCFDA
                     NNDGTCQASEFEVIHCAKNFLLLLTDGQWNRGGRPVQSACKISDDIEAESPDPVVPAY
                     WLHKKGFKNQITNIDGHVEAIYAIGLWLGGTGEQALKNIAMYGSFDRTNTWPGGTTGY
                     PQDSCGPIDDCCSTANCGKGSACTDLPSSSPTWDKDGNGIPDTFFNSNNATQIKEKMV
                     DIILDILRHVSSGSAVSILASGEGSGANLLQAVYFPRKSFGITEIEWVGKMHNLWYYV
                     DPLLQNSNIREDTVEDKKLNLSNDYIVRFFYDSATSETLVERYSDTDGDGAPNESLGI
                     GNFDDVKNLWEAGRVLWSRNLTTSPRNIYTQLNGNLTDFSIANASGLQSYLGVSTLTE
                     SQDIINYVHGIDKNGYRSRTVTIGGNTAVWKLGDIVNSTPRIQSFVPTNTYHLTPPSG
                     YSDTTYHQFINQPSYASKGVVYVGANDGMLHAFYLGNLKQKWSGQGTDEKMLLDGSDH
                     AKELWAFIPKNVLPYLKYLPDQDYCHLYSVDASSFLVDASINGDPNDNKTASSWKTIL
                     I"
     gene            complement(2574..2933)
                     /locus_tag="PK125_13285"
     CDS             complement(2574..2933)
                     /locus_tag="PK125_13285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB39118.1"
                     /translation="MKKSSKLTTFFVKENKKMNMLRRKKFCFFLASIFLSLFFVQISA
                     AQDHLFLTGIIRSFHPNSGTIRVDVTSEGCKGLREFREPEGAQGDMDALLVGKQIHFY
                     IDSSICERGKVYNILER"
     gene            complement(2938..>3145)
                     /locus_tag="PK125_13290"
     CDS             complement(2938..>3145)
                     /locus_tag="PK125_13290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB39119.1"
                     /translation="FCGWKGVVFSLVSGSVLGTVVGIPFMILKGKDTKYAIPFGPFLS
                     LGAIIYTFKGDNLIHLLFKVLFRE"
BASE COUNT          842 a          729 c          636 g          938 t
ORIGIN      
        1 gatgagtatt gtcttccaac tactggctgt tttgttgtca ttcgggtcac cgttaatgct
       61 tgcatcaacg agaaatgatg aggcatcaac agaatagaga tgacaatagt cctggtctgg
      121 aagatacttc aggtaaggaa gaacattttt gggaatgaaa gcccagagtt cttttgcatg
      181 gtcgctgcca tctaacaaca ttttctcatc tgtgccctga ccactccatt tttgctttag
      241 attgccaagg tagaaggcat ggagcattcc gtcatttgca cctacataca ccaccccctt
      301 gcttgcataa ctaggctgat tgatgaactg gtggtatgta gtatccgaat atccacttgg
      361 tggagtcaaa tggtatgtgt tggttggcac aaaagattgt atacggggtg ttgagttgac
      421 tatatcccct agtttccaaa ccgctgtatt tccgcctatc gttacggttc tgctcctgta
      481 tccgttcttg tcaatgccat ggacataatt gataatatcc tgtgactccg taagagtgct
      541 aacgccgaga tatgattgca ggcctgatgc gttggcaatg gaaaaatcgg tcaggtttcc
      601 gttcagttgc gtatatatgt ttcttggtga ggtggtaagg tttcttgacc ataaaaccct
      661 tcctgcttcc cacaggttct taacatcatc aaaattgcct attcccagac tttcatttgg
      721 tgccccatca ccatctgtgt cgctatatct ctctacaagc gtttcactgg tggcactatc
      781 ataaaagaaa cgcacaatat aatcattact tagattcaac tttttgtctt ccacagtatc
      841 ttcccgaata ttactattct ggagaagggg atcaacataa taccagaggt tatgcatctt
      901 cccgacccac tctatttcag tgatgccaaa ggattttctc gggaaataga ccgcttgcaa
      961 aagatttgcc ccgcttcctt caccggatgc aagaatggaa acagcgctgc ctgaagagac
     1021 atggcgcaat atgtcaagta ttatgtctac cattttttcc ttaatttgtg ttgcgttatt
     1081 cgagttgaaa aatgtatctg ggattccatt tccgtctttg tcccatgtcg gtgacgaaga
     1141 aggtaaatcc gtacaggcgc ttcccttacc acagtttgca gtgctgcagc agtcatcaat
     1201 tggaccgcat gaatcctgtg ggtaacctgt tgtacctccc ggccacgtgt ttgttctgtc
     1261 aaaagagccg tacatggcta tattttttaa cgcttgttcc ccagtacctc cgagccacaa
     1321 accaatagca tatatggcct caacatggcc gtcgatattt gtaatctgat ttttaaaacc
     1381 tttcttatgc aaccagtaag caggcaccac agggtcaggt gattccgctt cgatgtcgtc
     1441 acttatttta catgcggatt gtacaggacg ccctcccctg ttccattgac catcagtcaa
     1501 aagcagcaga aaatttttgg cacagtgtat aacctcaaat tcgcttgcct gacaggtacc
     1561 gtcgttattt gcatcaaaac acctgttcat ggggtttttc catttagtat tcacatctgt
     1621 ctgggggcca gggcctccgt attgcgggga tttctgcgaa aaatagttgt acgcatccca
     1681 taacgcagga ccggttgggg ttgaaccccc aggagcttcg taattgattg ctgttattac
     1741 attcttgtaa ggatgtgtga catcatatga tgcatcagaa gaagacgtga aatcacctat
     1801 gtatactgtt ctggtcacac cattagtatc ataaaacatg gcgcctatga tcggttttgg
     1861 agaaaggttt ttgagctgga acaacaggcc tccactattg cctgttatcc gtgcccatgg
     1921 aactttaact ttagtcgctc catcagagct caggagaatg catccgtcat tattacagct
     1981 taatttggag tttggatata cctccgggtc acacgtctca accgaatttg cgcagctttg
     2041 gagttttcct cctgtcagtg tccatcgaag gacatcaatc cttgtcattt tttgatagtt
     2101 caggtaatta ccggacgtta aagtgcagtt acccgatgac ttccatgctg tgcagcaggc
     2161 ccactttgtc ttcccacacc cctgtgcacc ctttacacaa tcaccgaggt tataattttg
     2221 gcatttccat tttgtgcatt ctttgacaca gggtaatccc gttggggttt caacgtatac
     2281 gttgcttgca ttcaattcat aaaactttgc agggtcaaag tagccttcgt atgtggtagt
     2341 ggcattgtat ccatcaaggt aaccatcact attactatca gtatctccat aagaataggc
     2401 tttccatccc atgcttccgc taacatcaat gacaagcaaa aggtttggat caatgggtgc
     2461 ctctattgaa gagggaagct ggcaatagtc agtcatatcc tgaccctctg agagtattgg
     2521 cacgaccagg agcatcaagc atattatgga tatacgaaaa gatatcttca tgactacctc
     2581 tctaatatgt tatagacttt tccacgctca cagattgagc tatctatgta aaaatgtatt
     2641 tgtttgccta caagtaaggc gtccatatca ccctgcgcgc cttcaggctc cctgaactcc
     2701 ctcaacccct tgcacccctc agatgttaca tcaactctga tagtccctga atttggatga
     2761 aaggatctta tgattccagt caaaaacaga tgatcctgtg ccgcgcttat ctgaacaaaa
     2821 aaaagtgaca gaaatatgga ggcaaggaaa aaacaaaact tttttctccg aagcatattc
     2881 atctttttgt tctccttcac aaaaaaagtt gttaacttcg atgacttttt cacgcaatca
     2941 ctcccgaaac aatactttaa aaagtaaatg tatcagattg tcacctttaa atgtatagat
     3001 aatagctccg agtgatagaa aaggcccgaa gggaatagcg tatttagtat ccttaccctt
     3061 taaaatcata aatggaatac caacaactgt gcccagcact gaaccggaga ccaggctgaa
     3121 aactacgccc ttccatccac aaaaa
//
LOCUS       DAOOMC010000199         3113 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdus sp. isolate AMR_MDS_2685
            NODE_38015_length_3113_cov_5.750164, whole genome shotgun sequence.
ACCESSION   DAOOMC010000199 DAOOMC010000000
VERSION     DAOOMC010000199.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864760
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdus sp. (wastewater metagenome)
  ORGANISM  Syntrophorhabdus sp.
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae; Syntrophorhabdus.
REFERENCE   1  (bases 1 to 3113)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3113)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,729
            Genes (coding)                    :: 2,708
            CDSs (with protein)               :: 2,708
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 7 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3113
                     /organism="Syntrophorhabdus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38015_length_3113_cov_5.750164"
                     /isolate="AMR_MDS_2685"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2699756"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..745)
                     /locus_tag="PK125_13295"
     CDS             complement(<1..745)
                     /locus_tag="PK125_13295"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF018076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HPB39120.1"
                     /translation="MRKTIEINIVPSLKNMHSYFRLNIRRLFLRGIVTILLMVVACYV
                     LWDRNTVFGTEAVSVNQKPKLDVPYEPTSYGIAKAMLAMADVTAKDLVYDLGCGDGRI
                     VIMAAKERKARGVGVDIDPVRIKESIENAKAAGVSNLVRFIEQDLFITDITPATVMML
                     YLWPEVNIKLRPKLLKDLKPGTRIVSHSHTMGGWKADATQNVEGHSLYLYIVPVNLTG
                     TWLWTGLNGKATNIRMTQKFQEVQGTLPGN"
     gene            complement(773..>1552)
                     /locus_tag="PK125_13300"
     CDS             complement(773..>1552)
                     /locus_tag="PK125_13300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB39121.1"
                     /translation="GGAGFLVFLIGLACLFGLLKLIQRYLFYLVKAGHVAVITELIHK
                     GSLPPNVNQIEFGKQTVSRLFKEISALFLLDQLISGTVKSFNRKVMDIAGKIPIPGID
                     GIARVAGSITRLAVTYIDEAILSFSLAKNDKNVWESAKKGVILYAQNWKPILTNAVIL
                     AVVSIVGFLLFVAIMLIPFGFLSFVTTNELLKAFFLFCALMIGYGLKVAIINPFCLVA
                     TIITYNRIIIGQEPNRDWEMKMEQFSDKFRQLKAKAAETVV"
     assembly_gap    1553..1562
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1959..>3113)
                     /locus_tag="PK125_13305"
     CDS             complement(1959..>3113)
                     /locus_tag="PK125_13305"
                     /inference="COORDINATES: protein motif:HMM:NF019607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HPB39122.1"
                     /translation="GQQVTSTMAANEPKLKRTVVLSGLREPWDIAFTPDGAMLFTEKC
                     RGLSVRLPNGMVKRLYGATGSDVVAPDFFCQGQSGMLGVAVDPNFADNHLIYIFMTSN
                     LNDRKTNRVVRLVLDAGYTTVNSRKDIITDIPYKSSTTLWGGAGAHSGGRIRFSPFDR
                     LLYVTTGDNHNGLLSQDLTRLGGKVLRVDRDGNAAPGNNTPAGGDSRIFTYGHRNVQG
                     IAFRPGTGQPFSCEHGPGHSDEVTPLVAGGNGGWDPKPDPGVSCMSNYCGYTSNRSDG
                     RPTSMTDLFKFPDALRPSWTNKGVSEGMGHCTFFNGKQWQGWDGRLAVGFLRGARIDI
                     LRLDAAGVTIGHTTVSGIPSQRIRSLVQAPDGTLYIATDGGEIWRLTPTE"
BASE COUNT          834 a          755 c          638 g          876 t
ORIGIN      
        1 tattaccggg caatgtgcct tgtacctcct gaaatttctg agtcattctg atgttggtgg
       61 ctttgccatt taatcctgtc caaagccagg tacctgttaa attaactgga acgatatata
      121 gataaaggct gtggccctcc acgttttgag tagcatctgc tttccatcca cccatggtgt
      181 ggctatgcga gactatcctg gtgcctggtt ttaggtcttt aaggagtttt ggtcgcagtt
      241 tgatgttgac ctcaggccaa agatagagca tcattactgt tgctggagtt atgtcggtaa
      301 tgaagaggtc ttgttcaatg aaacgtacaa gattggatac tccggcagct tttgcatttt
      361 caatactttc ttttatacga acagggtcga tatccactcc tacccctctg gctttccttt
      421 cttttgcggc catgattact attcgcccat ctccgcaacc aagatcgtac acaagatctt
      481 tagctgttac gtcagccatt gcaagcatag ccttggcaat cccgtaactg gtcggttcgt
      541 aagggacatc aagtttcggt ttttggttaa ccgacactgc ttctgtaccg aaaaccgtat
      601 ttctatccca caacacataa catgcaacaa ccatcagcag gattgtgaca attcctctta
      661 gaaataatct ccttatattc aacctaaaat aactatgcat attctttaaa gatggcacaa
      721 tatttatctc tattgtcttt cgcaaacctg tttcgaggtt ctcccatacg atttacacga
      781 ctgtttccgc tgcctttgct ttcaattgtc gaaacttgtc agaaaattgt tccattttca
      841 tttcccagtc tctattaggt tcttgtccga taattatcct gttgtacgtt ataattgtgg
      901 caacaaggca gaaggggtta attatagcca ctttcaatcc gtagccaatc atcagcgcgc
      961 aaaataagaa aaatgctttc aagagctcat ttgttgtcac aaaagaaaga aacccaaaag
     1021 ggattagcat aattgcaacg aataacagaa agccaacaat gctgacaact gcgagtataa
     1081 ccgcattggt aagaattggt ttccagttct gggcatagag gataacacct ttttttgcac
     1141 tttcccaaac attcttatca ttttttgcta agctaaaact gagaatcgct tcatcaatgt
     1201 aagttacagc aagacgtgtt attgatcctg caactctggc aataccgtcg atgccaggga
     1261 ttggaatctt acccgcaata tccatcacct ttctgttaaa ggatttcacc gtgcctgaga
     1321 tcaattggtc caatagaaac agcgctgaaa tttccttgaa taacctcgat acggtttgtt
     1381 tgccaaactc aatctggttt acgtttggtg gcaaagagcc tttatgaata agttcagtga
     1441 ttacggcaac atgccctgct tttactaaat aaaaaaggta gcgctgaatc agtttcaaca
     1501 aaccgaagag acacgctagt ccaatgagaa agaccaggaa cccagctcct ccnnnnnnnn
     1561 nntccaaaaa ttttggccat caacaatagt aaaccaagaa aaatcgctgc gcctacgccc
     1621 atcagtccat agattgccac catgaccccc aggtatgggg aagtcttcaa taggatgctc
     1681 cacgtttctt taaaatctaa cttcatagag gacctcttcg gttccacgaa aatttttcga
     1741 aattctcctg tattaatgac tttgattcac atctttccta aatatatttt cagggacacc
     1801 tttaaaagtc ccaaatttta ttataacctg tcatgaaaca tacttaagtt ttattaactc
     1861 aaaaactttt acttccaatt aacacttaag aaaaaccgcc ttttgcaata gaaccaattc
     1921 tcttaacaca accgaaccca tgcatacttt tcatctgttc actcagtcgg ggttaggcgc
     1981 catatctcgc ccccgtctgt ggcaatataa agggttccat caggtgcctg gacaagtgag
     2041 cgtatgcgct gtgaaggtat gccgctaacc gtggtgtgtc cgattgttac gccagcagcg
     2101 tctaaccgca gaatatcgat ccgtgcgccc cgaaggaagc caactgcaag acgaccgtcc
     2161 catccttgcc actgtttccc gttaaagaaa gtgcagtgtc ccattccttc ggaaacccct
     2221 ttatttgtcc aggaaggtcg cagggcatct ggaaatttga agagatcagt cattgaagta
     2281 ggtcttccat ccgacctgtt tgaggtatat ccacagtagt tggacatgca actcacgcca
     2341 gggtcaggct tggggtccca ccctccattg ccgccggcta ccagcggtgt cacttcgtca
     2401 ctatgaccgg gtccatgttc acaggaaaac ggctggccag tacctgggcg aaaggctatg
     2461 ccctgcacgt tacggtggcc ataagtaaag atgcgtgaat cgccgccggc aggtgtattg
     2521 ttgccagggg ctgcattacc atcgcgatct acccgcaaca ctttgccccc caaccgggta
     2581 agatcctgag ataacagacc attgtgatta tcgccggtgg taacatagag caacctgtca
     2641 aaagggctaa agcgaatgcg gcccccgcta tgtgccccgg cacctcccca gagagtggta
     2701 gaacttttat agggaatatc agtaatgatg tctttgcggc tgttgaccgt ggtatacccg
     2761 gcatccagaa caagtctgac cactctattt gtcttcctgt cattgaggtt tgaggtcatg
     2821 aagatataaa ttaagtgatt atcagcaaaa ttcggatcta cagcaacacc gagcatgccg
     2881 ctctgaccct ggcaaaagaa atcgggagca acgacatctg atcctgtagc tccgtataat
     2941 cgcttgacca ttccattcgg taggcgtacc gagaggccac ggcatttttc tgtgaatagc
     3001 attgccccgt caggggtgaa tgctatatcc cagggttctc gcagacctga tagcacaact
     3061 gttcttttca atttaggttc gttggctgcc atcgtggatg taacctgctg tcc
//
LOCUS       DAOOMC010000200         3065 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdus sp. isolate AMR_MDS_2685
            NODE_38797_length_3065_cov_4.290033, whole genome shotgun sequence.
ACCESSION   DAOOMC010000200 DAOOMC010000000
VERSION     DAOOMC010000200.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864760
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdus sp. (wastewater metagenome)
  ORGANISM  Syntrophorhabdus sp.
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae; Syntrophorhabdus.
REFERENCE   1  (bases 1 to 3065)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3065)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,729
            Genes (coding)                    :: 2,708
            CDSs (with protein)               :: 2,708
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 7 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3065
                     /organism="Syntrophorhabdus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38797_length_3065_cov_4.290033"
                     /isolate="AMR_MDS_2685"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2699756"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1336..1714)
                     /gene="tnpA"
                     /locus_tag="PK125_13310"
                     /pseudo
     CDS             complement(1336..1714)
                     /gene="tnpA"
                     /locus_tag="PK125_13310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011236098.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            1854..2285
                     /locus_tag="PK125_13315"
     CDS             1854..2285
                     /locus_tag="PK125_13315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB39123.1"
                     /translation="MLVNTRDGDAMTSEYRTAAVIKGQMSKFSGIIAKDLSKPKQRLI
                     REMVYGIQAAKDIKLSNIVRALKESIPFIKTEDRLSRNLDDEDFTAEINNQICRLGNT
                     KVMDDMVIAIDPGDIRKRHASRMEYLCKIHDGSEYEIGEGY"
     gene            2298..>3065
                     /locus_tag="PK125_13320"
     CDS             2298..>3065
                     /locus_tag="PK125_13320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB39124.1"
                     /translation="MAADIEHKKVIPLYLEAYSHEAKDFRSENDQIFKAIDTVSLHIG
                     NKGIYAIDRGGDRGKLYDKFLEKGKEKRFVIPLVKTRDLVHRGVRKNCHALATALPCP
                     HEAAIIKYEGGKEQKVTIRYASLPVKLPNHGYPLFLIVAKGFGKEPMMLLTNCPVKMK
                     VKETIWHVIEIYLTRWKCDESFRSIKQCYNLENIRVRHYTSIRNLVAIILAVSYFAAV
                     YLADNLKLKILIERVYLVSKRFFGVPTFFNYAIADGLY"
BASE COUNT          962 a          687 c          643 g          773 t
ORIGIN      
        1 tttgtaagga aggtacccga tcttcccctt atgacacaag atatgataca ctacccccgg
       61 gggtggggaa ttaacatggc ccttgacaga aggtaccgat tgatgacata aattatcggg
      121 actgtcatca ggagttgaat tattgaatag aacatcatac tccataaaga cattcctggt
      181 atggtcagac cgacaacccg aaccataacc ttttcgacac cacgaattac taaatatgca
      241 ggtaaatgca aggctagtat cgccagcgag ttcctaccaa caaaactaaa tatcatattc
      301 tctggaatag ccttgctgac tagtatacaa agtcctatac cggagaaact agccacatag
      361 aaatagaagt aactgcccat cctattggct cctaaattga caaagtcatt gaggtaggaa
      421 aataccagac ttgtagcaag cagaacaata aataataaaa atttgccaaa taatattctg
      481 ttagagagcg acattaatgc atccttagct aagaatccaa taccgaaaag tccaattcct
      541 gtaaaggctg ttccaaagct ccatggtagc ctgaaaggca ggtacagtct gtctaattgt
      601 ccaataatag aaatcaataa caaaaaaaag accgaacacg cacgtggctt tatatgcttt
      661 gaattgtgaa cttaatttat agcatagata aaaccctgct tctataataa agaggcaggt
      721 caagaaccaa agcggaggat tgtgtgtcat ccaaattata tcaggagacg aaataataac
      781 acccacaatt cctcccagaa cgtggggcgc atcgattgga ggcacccccg attggaaagt
      841 aacccaaaaa tgcaagagaa aataaagata aaataatgca ccagaagagt tcttgccttc
      901 tttgctaaaa gatctaaaaa atgcttgtac ttttgaggcg tgaatacggt gccagaagca
      961 aacagaaaca ggggtaaatg aaaggagtag atataatgta gtagtccctt tggaatctca
     1021 ttagaatggt gagcaaaaat tacaagaaac attcctatcg ccttcaggtt atcaatccaa
     1081 attatccttg atgtcttaac cgacattaaa tttatgtctc gcgtctccct tagtaacggc
     1141 tatctatata ataacatgaa aatcttaaca actctgtcta ataaatggat gatgataaga
     1201 taattggagt agctcccgtt ttaaccggac actcataaag ccaaaataag tcacttgacc
     1261 ccgggcaaag ccgggggctt gataatgtga accgctcaaa gcggttaaat actgtgagcc
     1321 gtctaaaagc agcttctatt cttcgaactt tttcagatga tcgagtttct tatcttcgtc
     1381 ctcttgcctt ttgatatata cacgaacaac atcttcatct aatcccgtcg tggatacaca
     1441 atatcctctg gcccaaaaat tctggccgat gaagttccgc cgtctgccgc acattcttga
     1501 tacccatatg gcgctcttcc ctttgagaaa acctatgacc taggctaccg catatttcgg
     1561 cggaattgat aatgagcata tgaacatgat ctgccacaag atgcccttcc actaccacac
     1621 cctctttttg tcgagccagt tcttttagta ttagtccaag cttttttctg atctgtccat
     1681 aaagcatctt cttcctgtat ttaggtatcc acacaacatg actggagttt ccttaatttt
     1741 cttgtcttac ggtcagtaac agatagtaat tattaacaaa tgactgtttt aacaaatcga
     1801 atctgtttct caccgaaaaa gtttcctgac ttttcttctg agttctggta gcaatgctgg
     1861 taaatacaag agatggagat gccatgacga gcgaatacag gacagcagcg gttattaagg
     1921 ggcagatgtc gaaattctca ggaatcattg caaaggatct ctcgaagccg aagcagagac
     1981 ttatcagaga gatggtctac ggaatacagg cggcgaagga catcaaattg tccaatattg
     2041 tccgcgcgct gaaggagtct attccgttca tcaaaacaga ggaccgtctc tcccgcaatc
     2101 tcgatgatga ggacttcaca gcagagatca ataatcagat atgccgtctg ggcaacacga
     2161 aggttatgga tgatatggtg attgccattg atcccggaga catacggaaa aggcatgcct
     2221 ccaggatgga atatctctgc aaaatccatg atgggagcga atacgagatc ggcgagggat
     2281 actgactctg caaagccgtg gctgcggata tcgagcacaa gaaggtcatt cccctttacc
     2341 tggaagctta ctctcacgag gcaaaagact tcagaagcga aaacgaccag atcttcaagg
     2401 ccatcgatac ggtgagtctc cacattggga ataagggcat ctatgccatt gaccgtggag
     2461 gagatcgggg gaagctctat gacaagttcc ttgaaaaggg gaaggaaaaa cgattcgtca
     2521 tccccctggt aaaaacacgg gaccttgttc accggggggt gagaaaaaac tgccacgctc
     2581 tggctacggc acttccctgt ccccatgaag cggcaatcat taaatacgaa ggcgggaaag
     2641 aacagaaagt cacgatcagg tatgcctcat tgccggtaaa actgccgaat catgggtatc
     2701 ccctgtttct tatcgtggcc aaggggttcg ggaaagaacc catgatgctt ctcacgaact
     2761 gtcccgtaaa aatgaaggtc aaagagacca tctggcatgt tatcgaaatc tacctcaccc
     2821 gatggaaatg cgatgaatca ttccgctcca tcaagcagtg ctataacctt gaaaacatcc
     2881 gtgtacggca ttacacgagc atacggaacc tagtggcaat catcctggcg gtttcctatt
     2941 ttgcagcagt ctacctcgcg gacaatctca agctcaagat ccttatagag cgcgtatacc
     3001 ttgtatcgaa aagattcttc ggcgttccca ccttcttcaa ctatgccatt gccgatgggc
     3061 tgtat
//
LOCUS       DAOOMC010000201         2986 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdus sp. isolate AMR_MDS_2685
            NODE_40238_length_2986_cov_6.253838, whole genome shotgun sequence.
ACCESSION   DAOOMC010000201 DAOOMC010000000
VERSION     DAOOMC010000201.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864760
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdus sp. (wastewater metagenome)
  ORGANISM  Syntrophorhabdus sp.
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae; Syntrophorhabdus.
REFERENCE   1  (bases 1 to 2986)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2986)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,729
            Genes (coding)                    :: 2,708
            CDSs (with protein)               :: 2,708
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 7 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2986
                     /organism="Syntrophorhabdus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40238_length_2986_cov_6.253838"
                     /isolate="AMR_MDS_2685"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2699756"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(77..478)
                     /locus_tag="PK125_13325"
     CDS             complement(77..478)
                     /locus_tag="PK125_13325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB39125.1"
                     /translation="MEPIKVVVRYADGKLLKGMTQDFFPNKDRFHLRSDTTNPEEPVE
                     IPVRELKAIFFVKDFIGNQAYTERKEYNNGDKVQGRKVEVVFVDGERLVGSTLGYDPN
                     RLGFFLFPVDPESNNIRIFAVTAAVKSVRYI"
     gene            683..1921
                     /locus_tag="PK125_13330"
     CDS             683..1921
                     /locus_tag="PK125_13330"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HPB39126.1"
                     /translation="MDDRSVYKSIGREEDLKGISRRVSQISVSAIKQMPVLAREVAGA
                     VSLGQGIPSFGTPAFITKVISEALTKDEWIGKYSLQPGLPELKHEIARYLRWKKGISV
                     DAETELFVSCGAMEALAAGIATVVDRGDEVLIPTPNYASHIEQVLFAEGIPVFVPLLE
                     EQGWRLDVEGFRKAITKKTRAIIICNPMNPTGAVFSKEDILAVAQLALEKDLFVIADE
                     AYDFLIYDNHEYLSCASIPELKDRLIGAFSFSKMYCMTGWRVGFMYASSKIINQALKV
                     HDAFAICAPTVSQCAALAALRATNGKDGPGDNFIKNLVHALGQRRELTCARLDRLKSL
                     FSYQIPRGAYYVFPKIVGSKINSMELAIRLLYEARVISIPGNGFGPSGEGHIRFSFGG
                     TEDEINTAFDRIEEWAQINL"
     gene            complement(1911..2726)
                     /locus_tag="PK125_13335"
     CDS             complement(1911..2726)
                     /locus_tag="PK125_13335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB39127.1"
                     /translation="MISRLFWAALVIIFMLPNMAFAHVGLEGTVDVRIISDGGREFVK
                     FRAYPQCQEDGRYFYVQASKGERYSIQVTNTLGRRIGVVIAVDGRNIIDGKKSDLRRN
                     ERMYIIGPYGTNTFEGWRTAVDRTNRFYFTEQSDSYAEKVFADASAMGTIAIAVFREK
                     TRDIVHLQGPSSVMKEAPTAEAAPGTSRGAHSAELAEKGKREQAGTGFGETTYSPTRS
                     VNFEPERTAIDRIVMKYEWRSELCRKGIIQCDPRNRLWPGGQEFAPIPRDFRG"
BASE COUNT          813 a          683 c          692 g          798 t
ORIGIN      
        1 cgttagcatg attatacatc aaaacaagaa acaggtcatc ctgtaagatt gttagaggac
       61 tgaagaggcg ggactgctaa atgtaacgga cgcttttcac tgctgcagta actgcaaata
      121 tgcggatgtt attgctttca ggatccacgg gaaaaaggaa aaaaccaagc ctgtttgggt
      181 catatccaag agtggagcct acgagccttt ccccatcaac aaaaactact tcaactttac
      241 ggccttgaac tttatctcca ttgttgtatt cctttctttc agtataagcc tgattgccaa
      301 taaagtcctt tacaaagaag atcgctttta gctcacgaac aggtatttca acaggctcct
      361 ccgggttggt tgtgtcagag cggagatgaa agcgatcttt gtttggaaaa aaatcctggg
      421 tcataccctt taacagctta ccgtctgcat agcgtacaac aacttttatc ggttccatca
      481 ttgccccctg tatcatttaa aggaggattc gttattgaat cagcctttac atgttatcgt
      541 cagtaaaaat aaaatactaa aggtctgtgg aggatgatgc gggcagccag gaattacagt
      601 cacgggcagt gatcgaatca gtacttgttt ggatagggta ggtgcgatca gtttatggta
      661 agctaaccaa gggaggatta atatggatga tcggtctgtg tataaaagca taggaagaga
      721 ggaagaccta aaaggcatat cacggagagt atcgcaaatc tccgtatccg ctatcaagca
      781 gatgccagtt cttgcccggg aagttgccgg cgctgtttca cttggtcagg gaattccttc
      841 ttttggaacc cctgcgttca tcacgaaggt tataagtgaa gcattgacaa aggatgaatg
      901 gattggaaaa tattctttgc agccgggact tccagaactc aaacatgaga ttgccaggta
      961 cctccggtgg aaaaaaggta tttctgtgga tgcagaaaca gaactcttcg taagttgtgg
     1021 tgccatggaa gcactggcag cgggcattgc aacggtggtg gatcgtggag atgaagttct
     1081 cattccaaca ccaaactatg cttcccatat tgaacaggtg ctttttgcag aaggcatacc
     1141 tgtttttgtg cccctccttg aagagcaggg atggaggctt gatgttgaag gcttcagaaa
     1201 ggccattaca aaaaagacca gggccataat tatctgtaac cccatgaacc ccactggagc
     1261 ggttttttca aaagaagata tattggcggt tgcccagctt gcactggaga aagatctttt
     1321 tgtcatcgca gatgaagcct atgactttct catctatgat aatcatgaat acttgagttg
     1381 tgcctctata ccggagttga aagacaggct cataggagct tttagcttct ccaagatgta
     1441 ctgtatgaca ggatggagag taggtttcat gtatgcctcc tcaaagatta taaaccaggc
     1501 cctgaaagtg catgatgcct ttgccatctg tgcgcccacg gtctcccaat gcgcagcact
     1561 tgcagcttta cgggctacca acgggaaaga cgggccaggg gacaacttca tcaaaaatct
     1621 cgttcatgcc ctgggtcaaa ggagagagct gacgtgcgca cgcctggaca ggctaaaatc
     1681 tcttttttcc tatcagatac ccaggggggc ttactatgtt tttccaaaga tcgttggatc
     1741 caagatcaat tccatggagt tggcaatccg gcttctctat gaggcacgtg tgataagcat
     1801 tcccggaaac ggttttggcc cctcaggaga ggggcacata cgattctctt ttggcggtac
     1861 agaagacgaa atcaacaccg cttttgaccg aatcgaggaa tgggcacaga tcaacctctg
     1921 aaatcccgtg gaataggggc gaattcctgg ccacccggcc acaacctgtt tcttgggtca
     1981 cactggataa ttccttttct gcaaagctca gatctccatt cgtatttcat gacaatcctg
     2041 tcaatagcgg ttctttccgg ttcaaaattc acagaacgtg ttggagaata tgttgtttca
     2101 ccaaatccag tacctgcctg ctctcttttg cctttttctg ctaactcagc agaatgtgct
     2161 cctctggagg ttcctggtgc ggcctctgca gtgggagctt ctttcatgac tgaagacggg
     2221 ccctgtaaat ggactatgtc ccgggttttt tccctgaata cagcaatagc aatggtaccc
     2281 atagccgagg cgtctgcgaa caccttttcc gcgtacgagt cagattgctc ggtgaaatag
     2341 aagcggttcg tcctatccac agctgtcctc caaccctcga aggtattcgt tccatagggg
     2401 ccaatgatat acatgcgttc gttacgcctg aggtcggatt tcttaccatc aattatgttt
     2461 cttccgtcta cagcgataac gacccctata cgtctaccca atgtgtttgt aacctggatt
     2521 gagtaccgtt caccctttga tgcctgaaca taaaaatacc tgccatcctc ctggcattgc
     2581 ggataagccc ggaatttgac aaattcacgg cctccatcag aaatgatacg gacatcaaca
     2641 gttccctcta atcccacatg tgcaaaagcc atattcggaa gcataaagat aatcaccaat
     2701 gctgcccaaa aaagtctgct tatcataacc ttacctccat ctttgtattt gctgttggtt
     2761 tgttagacaa taggtaaggt aaaaaagttc ccgggaaggc aaaagactgc tttgtcagga
     2821 caaagggatg gcatctttgc aagggcaggg tttttgtgtc aggagcaaac gatctttacc
     2881 acaaaggtaa acacccacaa ggcgcaggtc ctctctactc gtgattaacg ctccatctca
     2941 agagcaggca tgaagaacta tcaatataac ggttgccagt acatac
//
LOCUS       DAOOMC010000202         2953 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdus sp. isolate AMR_MDS_2685
            NODE_40852_length_2953_cov_5.574879, whole genome shotgun sequence.
ACCESSION   DAOOMC010000202 DAOOMC010000000
VERSION     DAOOMC010000202.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864760
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdus sp. (wastewater metagenome)
  ORGANISM  Syntrophorhabdus sp.
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae; Syntrophorhabdus.
REFERENCE   1  (bases 1 to 2953)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2953)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,729
            Genes (coding)                    :: 2,708
            CDSs (with protein)               :: 2,708
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 7 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2953
                     /organism="Syntrophorhabdus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40852_length_2953_cov_5.574879"
                     /isolate="AMR_MDS_2685"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2699756"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1461..1976)
                     /gene="hpt"
                     /locus_tag="PK125_13340"
     CDS             complement(1461..1976)
                     /gene="hpt"
                     /locus_tag="PK125_13340"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR01203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HPB39128.1"
                     /translation="MLKKLFSREEIDRAVKRLATEIENDYDNEQIIFICLLKGSFMFT
                     ADLIRKIRNPLRVDFLRASSYGCGTNSKGEVTIGKDIEEGIESENVIIVEDIIDSGLT
                     LTRVRNMLQEKNPKSLRICALLDKRARRKVDIEGDYVGFTLEDGFVVGYGIDYAEEYR
                     NLPDIFVIVGE"
     gene            complement(1986..2642)
                     /locus_tag="PK125_13345"
     CDS             complement(1986..2642)
                     /locus_tag="PK125_13345"
                     /inference="COORDINATES: protein motif:HMM:NF024874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HPB39129.1"
                     /translation="MKIKWYGHAAFKITTDSGVTVIIDPYQSGAFGGALSYGKISEPA
                     DIVLSSHDHDDHNYRDDIKGTYKYFNKEGNYNEMGIKIEAIPCYHDPAKGKERGNNLI
                     FVIEADGLRLAHMGDLGHTLSDDLIKKMGKIDVILVPVGGFYTIDADEAGKVVKDLNP
                     RITIPMHFKTPKCEFPIGGVELFTSGKKRVTVVNEHEIEVTKDGLPEDGEILVMKYAL
                     "
BASE COUNT          796 a          686 c          501 g          970 t
ORIGIN      
        1 cttcaggtcg cctatttgtc tttctagtct ttttagttct tgctcaagcc cttccctgtt
       61 catctcctgg tagctcatca atccaaagtc taaatcaatt atcaccaact gtccagacta
      121 ttagcgctat gcctcaagca gttatttgta gttcttttcc aataagttca tgttttttta
      181 agtcttagct ccatacaatt agtattaatt gcccggatgt acatatataa taggtattta
      241 aaacagtcgc atttttcaag ttggcagatt tgtactgacg ttttaatgta caaataatcg
      301 caacgagtag cttattaatc caataaccta cgttttttat gctttttccg aggtagaaag
      361 ctaggattac tgtaatccct gctgttttct gaagaaacac caacaacatg ctttatggtt
      421 cggcctgaat ttatatatat ggtttgccgt gtgttagaat atacccacgg ttgtgccttt
      481 gctgactcct aagaaaatcg ttcgattctt ttctaccatt cgccatcaca agaaaccaca
      541 gctaccacaa aatctgcatt tactcacaag aagctattaa taaaaccctt ctttcccaat
      601 tgaatcgatt gtctcacatg caatagaccc ggcaagcacc gcagctttgt gggaatcaac
      661 aggaaagtta ttgcttttgt tgcacgatcg ttgccaatgc agaacgattc caactctcaa
      721 cttaatgcta cctgtgtaat aaaaacctct cgcaaaggtg tttgcagagg aaaatcttta
      781 cacctggatg ctcaacgcac gaaagatgtg agaaacattc acctgtactg tattgatata
      841 tctttctaaa tggatctcct ggatcacgat tgtctccttc agcatagggg gcaccgttcc
      901 ttatgacact tcctaaaaaa tatgaccctg ataatccgcc tgtggtactg attctgaaat
      961 aacatccttg ccttctttac aggcccccat gagcgtatgt ctggaaaaac gacagatgac
     1021 atcctttacc actcagtaag gttttgcaaa gaatctatcc tttctgggaa actaaaaatc
     1081 cctttcctgc actgaatctg gaggcacctg aggcaatgaa taaaggaatt gtgggttaaa
     1141 actgttttac cagcagttac tgtgatggga ttaagggcaa ggcttgttcg cgtttatggt
     1201 tcatgtgggt agagtctaag tgtgtttttg ggaacaatga aaaaatcgag ctggatttat
     1261 agtatttgag tgatggttgc gtttcacgtg aaacaggttt caatctttgt tttctttgta
     1321 tgttcttatt ctattttcat ttgcaatatc agtatgttat gtggttttca tctgattttt
     1381 taagtgtttt tgccccttat tcagcgcaag aactttatct cttacatgat catgagaaaa
     1441 ctgaacaatg tgtgtgtcaa ttattcaccc acaatcacaa aaatatccgg aaggtttctg
     1501 tactcttctg cataatctat cccatacccg acaacaaaac cgtcttcaag cgtaaagccg
     1561 acatagtccc cttctatgtc aacctttctc cgcgctctct tgtcgaggag ggcgcatatc
     1621 ctgagtgatt ttggattctt ttcctgcagc atattacgga cacgggtcaa ggtcaggcca
     1681 gagtctataa tgtcttccac aataatgaca ttctcactct caatgccctc ctcaatgtct
     1741 ttcccaatgg tcacttcccc tttggaattt gttccacacc cataggatga ggccctaagg
     1801 aaatctaccc gtaaagggtt acgaatcttc cttataaggt cagcagtaaa cataaaagac
     1861 cccttgagga gacatataaa aataatttgt tcattatcgt aatcattctc aatctctgtt
     1921 gcgagccttt tcacggccct atcaatctct tctcttgaaa aaagcttttt taacacgttc
     1981 aaaccctata gtgcatattt cataactagg atctctccat cctctggcaa accatccttc
     2041 gtcacttcaa tttcgtgctc gttgaccacg gtaactcttt tttttcctga tgtaaacagc
     2101 tctactccgc caataggaaa ttcacatttg ggtgttttaa aatgcatggg gatcgttatc
     2161 cttgggttta gatctttcac tacttttcct gcctcgtcag catctatggt ataaaagcca
     2221 cccacaggca ctagaatgac atcaattttt cccatctttt taattaaatc atcagaaagc
     2281 gtgtgcccca agtcccccat atgggccaat cttaacccat cagcctcaat cacaaatatg
     2341 agattgttac ctctctcttt gcctttagcg ggatcatggt agcacggaat ggcttcgatc
     2401 ttgataccca tttcattgta atttccttct ttgttgaagt atttatacgt tccctttatg
     2461 tcgtccctgt aattatgatc gtcatgatcg tggcttgaaa gaacaatatc ggccggctcg
     2521 ctgatcttcc cgtacgacag agcaccacca aatgctccgg attgataagg atcaataatt
     2581 actgtcactc cactatctgt tgtgattttg aatgctgcat gtccatacca cttaatcttc
     2641 ataaaaacct ccttcttttt ctctccatct ctttgcctga cccttcctgt ttagcttatt
     2701 cattaccttt cgtgctagtg ctttcttgtt tcattcactg tttctcaaaa cttctgacaa
     2761 tgccttctcc atattacact tcgatctact acgtgtcaac tctcatcaac atccatcaaa
     2821 aattctcgtt tgcaacgaag ccaaaaaagt tttttcaaac aaggtcgttt aaaaacggag
     2881 tcagctttac gtcgaccggg acaataaatt atgataatat ccttatacaa ggtgcaggat
     2941 gaagcccaaa cgc
//
LOCUS       DAOOMC010000203         2845 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdus sp. isolate AMR_MDS_2685
            NODE_43076_length_2845_cov_3.701075, whole genome shotgun sequence.
ACCESSION   DAOOMC010000203 DAOOMC010000000
VERSION     DAOOMC010000203.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864760
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdus sp. (wastewater metagenome)
  ORGANISM  Syntrophorhabdus sp.
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae; Syntrophorhabdus.
REFERENCE   1  (bases 1 to 2845)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2845)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,729
            Genes (coding)                    :: 2,708
            CDSs (with protein)               :: 2,708
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 7 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2845
                     /organism="Syntrophorhabdus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43076_length_2845_cov_3.701075"
                     /isolate="AMR_MDS_2685"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2699756"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..740
                     /locus_tag="PK125_13350"
     CDS             <1..740
                     /locus_tag="PK125_13350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB39130.1"
                     /translation="LGTVTTLFEKNQYQTATKEKQLLIENAYLSILAACTTDTWERCW
                     HPDFTAIGLVNRLFLVCGGMEKLVPIPPVLEIGRWKNLLQNTRAVVKLAQKIHKYELT
                     PESVRLYDQWYRNTLDHKSVHSVRLDAYALRLMLLLAIVDERYQIDTDIVQDVIQLVT
                     WEHAVRQQLDPIDADNEQAKIELRIRRNLLLGPLTKRALQQKTGAHRSGTWLWHNALS
                     GLLAHNEIAYNNKTKEYYILSNTEELR"
     gene            1017..1286
                     /locus_tag="PK125_13355"
     CDS             1017..1286
                     /locus_tag="PK125_13355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB39131.1"
                     /translation="MKNVISNKCRDTQLIELINDLYEESQSLGFITNTAEIPKEIAKE
                     LDRVAGVLRGLYLILEKATITIDQKTFDVYFEKIIKHRHENWQVI"
     gene            1342..1869
                     /locus_tag="PK125_13360"
     CDS             1342..1869
                     /locus_tag="PK125_13360"
                     /inference="COORDINATES: protein motif:HMM:NF015550.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051276 - chromosome organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HPB39132.1"
                     /translation="MKKRKIDDTLLLEMLNEGKQQKEIAAWFKVSPAAVCKRIKRLLS
                     PTPESILDKYNLTDQQKMFVVEKAKGRSNTEAALESYEASSRKSAKVIGSQLMAEPEI
                     KMALNELMDTYLPQHYRIRKLRTHADNPDPTVSLKALDLSWKLDGSYAPEKHAHQILG
                     FTLIDLELSNRKEED"
     gene            1996..2280
                     /locus_tag="PK125_13365"
     CDS             1996..2280
                     /locus_tag="PK125_13365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB39133.1"
                     /translation="MEAAERETIITWNDVEDTVSVFTCHRKIMAKMERLGATVKDRCV
                     MNGRVRHIEFEIDKGKITISINAKRRGTATQIENLKRARALSPIGKKKVA"
     gene            2384..2689
                     /locus_tag="PK125_13370"
     CDS             2384..2689
                     /locus_tag="PK125_13370"
                     /inference="COORDINATES: protein motif:HMM:NF024139.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HPB39134.1"
                     /translation="MKIELEQSDIQAIAEKVVELMKPYLSQRSDLQENTIFDVPGLCK
                     YLHVTSKWIHERTHLKQIPHYKLSNKQLRFRKKDIDKWLSSMRTSVINPLPKVRLIK"
BASE COUNT          952 a          567 c          619 g          707 t
ORIGIN      
        1 tgcttggaac agtgacaacg ctgttcgaaa agaaccaata ccagacagca acaaaggaaa
       61 agcaacttct aattgaaaac gcatacctat caatattagc ggcttgcaca acggatacat
      121 gggagagatg ctggcatccg gattttactg caataggact tgttaatcga cttttcttgg
      181 tgtgcggcgg catggaaaag cttgtcccta ttccacctgt acttgagata ggccgttgga
      241 aaaatctttt acagaatacc cgggcagttg tgaaactagc gcagaaaatt cataaatatg
      301 aattaacacc ggaaagcgta agattgtatg atcagtggta cagaaacacg ttggatcaca
      361 aaagcgttca ttctgtccgg cttgatgcct atgcattgcg gctcatgctg cttcttgcga
      421 tcgttgatga acgttatcag atcgatacag atatagttca agatgttatt cagcttgtga
      481 cctgggaaca cgctgttaga cagcagcttg atcctatcga cgcagataat gaacaggcaa
      541 agattgaatt gaggattcgg cgaaacttgt tattagggcc tcttacaaaa cgtgcgctac
      601 aacaaaaaac tggtgcacac cggtctggta cttggctatg gcataatgct ttgagcggct
      661 tgcttgctca caatgaaatc gcctacaaca ataaaacgaa agaatattac atacttagca
      721 acactgaaga gctgcgatga aaagtgtaga caattccgta gacatactta tcttatcatg
      781 taacatcttt aaattattaa tgattaatag ttttcactta tatagtagca ccatactttt
      841 tagaactcgg gtgtgtgttg atcaatacta ttgtgtaagt accggaaata tcaaaagaaa
      901 acaccgtttt gataggtgtc tacacttttg tctacaaaat catcggttaa tgaaaacccg
      961 atatatgaaa tgcgaacaag tcccggccct ttggcgggca ttaataggag gtcattatga
     1021 aaaatgttat ttccaacaag tgcagggaca cacagctaat tgaacttatc aacgatttat
     1081 acgaagaatc acaaagttta ggatttataa ctaataccgc agagataccg aaagagatag
     1141 caaaagaatt agaccgggtc gcaggcgtgt tgcgcggcct gtatctcatc cttgaaaagg
     1201 ctacaataac gatagatcag aaaacctttg atgtgtattt tgaaaaaatt atcaagcacc
     1261 gacatgaaaa ctggcaggtt atttaaacaa caaaataacc acgattaacc gatttaagta
     1321 tgcctgtgag gttaagaggt tatgaagaaa agaaagattg atgacaccct gctcctggag
     1381 atgctgaacg aaggaaaaca gcaaaaggag attgctgcct ggttcaaagt atcccctgct
     1441 gctgtatgca agcggattaa acgcctgctt tcacctaccc ctgagagtat ccttgataag
     1501 tacaatctga ctgaccagca aaaaatgttt gtagttgaga aggcaaaagg gagatcaaat
     1561 actgaggcag cacttgaaag ctatgaggca tcctccagaa aaagcgcaaa ggttatcggc
     1621 tcacaattga tggcagaacc tgaaatcaaa atggctctca acgaactgat ggacacctac
     1681 ttaccccaac attaccggat aaggaagctt cgcacccatg ccgacaatcc agaccccaca
     1741 gtgagcctca aagctcttga tctctcatgg aagttagatg gcagttatgc ccctgagaaa
     1801 cacgcccacc agattcttgg ctttaccctt attgatcttg aactgtcgaa caggaaggag
     1861 gaggattaac cttgaaaaat agtgcacgca gtcagaaagg ggtatttcat gcaatatgct
     1921 gtgactataa aaacccctcc cctcccccct ggacacagat gaagaacacc ataaaatcca
     1981 taaggagatt gacaaatgga agctgctgaa agagaaacaa tcattacatg gaacgatgta
     2041 gaggatacag tcagcgtctt tacctgccac aggaagatca tggcaaagat ggagcggtta
     2101 ggggccacag tcaaagatcg ctgtgttatg aatggcaggg tgcgccacat cgaatttgag
     2161 attgacaagg gcaagataac aatcagtatt aacgcaaaaa gacgtggaac agccacacaa
     2221 attgagaacc taaagagggc aagagcatta tcaccaatcg gtaagaaaaa ggtggcgtga
     2281 agatgtgtgt ctgctatagc ccgtatggtt acagtctggt tgcatatcct ttcagaaaaa
     2341 aggatagcgg gtgcccttcc agggcacata ttcaaggttg tctatgaaga tcgagcttga
     2401 acagtcagac attcaggcaa tagcagagaa ggttgtggaa ctcatgaaac catacctttc
     2461 acagagatca gaccttcaag agaatactat ctttgatgta ccaggtctct gtaagtatct
     2521 tcatgtaaca tctaaatgga ttcatgaacg gacacatttg aaacaaatac cacactacaa
     2581 gctgtcgaat aagcagttac gattcaggaa gaaggacatt gacaaatggt tatcctctat
     2641 gagaacgtct gtgattaatc ctctaccaaa agtaaggctt atcaagtgag cataacggat
     2701 attgtaaccg ttgaatacct tattaatccc tttaatatca agtcatatca tgacactgta
     2761 tagtaaaatg tatagtatga attctcaaca aagaacccct gggttggcct atattctctt
     2821 gagggggtat atagaggcta agaat
//
LOCUS       DAOOMC010000204         2839 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdus sp. isolate AMR_MDS_2685
            NODE_43191_length_2839_cov_4.097701, whole genome shotgun sequence.
ACCESSION   DAOOMC010000204 DAOOMC010000000
VERSION     DAOOMC010000204.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864760
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdus sp. (wastewater metagenome)
  ORGANISM  Syntrophorhabdus sp.
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae; Syntrophorhabdus.
REFERENCE   1  (bases 1 to 2839)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2839)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,729
            Genes (coding)                    :: 2,708
            CDSs (with protein)               :: 2,708
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 7 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2839
                     /organism="Syntrophorhabdus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43191_length_2839_cov_4.097701"
                     /isolate="AMR_MDS_2685"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2699756"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(159..1247)
                     /gene="arsB"
                     /locus_tag="PK125_13375"
     CDS             complement(159..1247)
                     /gene="arsB"
                     /locus_tag="PK125_13375"
                     /inference="COORDINATES: protein motif:HMM:TIGR00832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008490 - arsenite secondary active
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACR3 family arsenite efflux transporter"
                     /protein_id="HPB39135.1"
                     /translation="MDDTARRLSFLDRFLTLWIFLAMFIGVACGYFFHGIVDFWNQFQ
                     AGTTNIPIAIGLILMMYPPLAKVRYEELGEVFKNWKVLLLSLIQNWVVGPVLMFILAI
                     VFLRGYPDYMVGLIMIGLARCIAMVLVWNDLAQGDTEYCAGLVAFNSIFQVLFFSVYA
                     YFFITVLPPLIGLKGVVVSITIGKIAESVLTYLGIPFIAGVITRLVLIKIKDIKWYHE
                     RFIPKISPITLVALLFTIFVMFSLKGEYIVKIPLDVIRIAIPLVIYFVVMFLFSFYMS
                     IKAKAHYGQAATLSFTAASNNFELAIAVAVAVFGINSGQAFAAVIGPLVEVPLLINLV
                     YVSLWIKRKYFHYAMETPLGICYYTCKQ"
     gene            complement(1403..1615)
                     /locus_tag="PK125_13380"
                     /pseudo
     CDS             complement(1403..1615)
                     /locus_tag="PK125_13380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417958.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
     gene            1756..>2839
                     /gene="hgcA"
                     /locus_tag="PK125_13385"
     CDS             1756..>2839
                     /gene="hgcA"
                     /locus_tag="PK125_13385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_252989029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042192 - methylmercury biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:1804..1806,aa:Sec)
                     /transl_table=11
                     /product="mercury methylation corrinoid protein HgcA"
                     /protein_id="HPB39136.1"
                     /translation="MQFVPTKPVELDCTPCUGPSEKRGPVRPPRLDQPFVTGTVETAA
                     GIVPRVSKVYSRADRIGAYKVRWGIGRMNYRVDAGLYAIGEPTAESPVFVTANYKLTF
                     DLLRRAVASIDSWILVLDTLGINVWCAAGKGSFGNDELLDRIESSRLADVVSHRKLVL
                     PQLAAPGVAGHKVKARSGFQVIYGPVSAEDIPEFLKNGFKAEERMRTKCFGFMDRLAL
                     VPMEVVPAVKSGLLAAAVLFVLCFLFAGFSTLKALEYFIIPAVSIGTSIIAGAVLTPL
                     FLPWLPGRAFSIKGFVIGLLSAFTVVSFVHNPGVLETAVLLISIPAMAAYLAMNFTGS
                     STFTSLSGVRKEICLALPLEIGGTGAG"
BASE COUNT          798 a          632 c          631 g          778 t
ORIGIN      
        1 tacatccttt ttgtcatcct cttcaattta cacaaaagat tttacactac ctatattcat
       61 tttcctggat ttagacagaa ggtttcgaga gttggctagt atacctggtg gcatagaggg
      121 tatttgtgaa aggaaatatt actacctgta gtctgcattc attgtttgca cgtgtaatag
      181 catatcccca gtggtgtttc catagcatag tggaaatatt tgcgtttaat ccacaatgat
      241 acgtaaacaa ggttgatgag taaagggact tcaacaaggg gtccgatgac tgctgcgaat
      301 gcctggccag agttgatgcc gaagaccgct actgccacag caattgcaag ctcaaaattg
      361 ttcgatgctg ccgtgaaaga cagtgttgct gcctggccgt agtgagcttt cgccttaata
      421 ctcatgtaga aagaaaagag gaacataacc acaaaataga tgacaagggg aattgcaatt
      481 cttattacat ccagaggtat cttgactata tattctcctt tcagggagaa cataacaaaa
      541 atcgtaaaaa gaagagctac aagggtaata ggacttatct taggtataaa tctctcgtga
      601 taccatttaa tatctttaat cttaatcagt acaagacgtg taataacacc ggcaataaaa
      661 gggataccca ggtatgttaa cacgctctct gcaatcttcc cgatggtaat actgaccacg
      721 acacctttca agcctataag gggcgggagc accgtgatga aaaagtaggc ataaacagaa
      781 aagaaaagaa cctgaaaaat agagttgaag gccaccaacc cggcgcagta ttcagtgtct
      841 ccttgagcca gatcattcca gacaaggacc attgcaatgc atcgcgcgag tccgatcatg
      901 ataaggccca ccatataatc aggatatcca cggagaaata caatcgcaag aataaacatg
      961 agtacggggc caactaccca gttctgaatg agggaaagca ggagaacctt ccagttctta
     1021 aaaacttcac caagttcttc gtaccttacc ttggcaagag gaggatacat catgaggatt
     1081 aggccaattg caattggtat gttcgttgtg cccgcttgga actggttcca gaagtcgaca
     1141 atgccatgaa aaaaatatcc acacgctacg ccgatgaaca tggccagaaa aatccacagg
     1201 gttaagaaac ggtcgagaaa agagagcctt cgagcggtat catccatact cacgccctcc
     1261 cacagcaccc gcccaccata tcggcaaatt ctgcgggcct cgactcgata aatgcttcca
     1321 accgttgtcg gtcgttgact gtctcatcac ctacccgttc agatatggaa aggaaaagta
     1381 atcgtttgaa caagtctgat tcgttgagac ggtaatgcat ccacttccct tctcgccggt
     1441 ccttcacgag tccagcctcc ttgaggatct tcagatgaaa agaaatcttg ggttgagaca
     1501 tatcaatggc ggctacaatg tgacatacac acagttcgcc attttcaaga agcttaacaa
     1561 tcctcagccg cgtttcatcg gataatgcct tgaatactga taagagcccc ttcacttcaa
     1621 cctccaatca tatcaattat aataaatata tatattagtt atatcttgga atgtcaatgt
     1681 ttaattaaat atatattaat atgttttgat atgtttatct tgacaatatt taatgaattg
     1741 ttttatattc caagtatgca attcgtgcca acaaaacctg ttgaacttga ttgtacacct
     1801 tgctgaggtc cgtcagagaa gcgaggtccg gtaagacctc ctcgccttga ccagcctttt
     1861 gtcacaggaa cggtagaaac cgctgctggt attgtcccca gggtatcgaa ggtttatagc
     1921 cgagcggacc gcatcggggc ctataaagta cgatggggga tcgggcggat gaattatagg
     1981 gtcgatgcag gcctttacgc aataggtgaa ccaactgcag aatctccagt ctttgtcact
     2041 gcaaactata aattgacatt cgatttgctt cgaagggctg tcgcatcgat agacagctgg
     2101 attcttgtac ttgacacatt gggtattaat gtgtggtgtg ccgcaggaaa gggctcattc
     2161 ggtaatgacg agttacttga ccgtatcgaa tcgagtcgtc ttgccgatgt cgtatctcac
     2221 aggaagcttg tacttcctca acttgccgct cccggtgtgg cgggtcacaa agtgaaggca
     2281 cgttctggct ttcaggttat ttacggacct gtgagcgctg aagacatacc tgaatttctc
     2341 aaaaatggtt tcaaggcgga ggaacgcatg aggacaaaat gcttcgggtt tatggacaga
     2401 ctggcgcttg taccgatgga agttgttcct gctgtaaaat caggattact tgccgctgca
     2461 gtgctctttg ttctctgctt tctttttgca gggttctcca ctttgaaggc actcgaatat
     2521 tttataatcc ccgctgtatc gattggaact tcaatcattg caggcgcagt actcactccg
     2581 ctttttcttc catggcttcc tggtcgtgcc ttttcaataa aagggtttgt gatagggctg
     2641 ctatcggcct ttacagtcgt ttcctttgtg cataatccag gtgtgctgga aacagcagtc
     2701 ctccttattt caattcctgc catggctgcc tatcttgcaa tgaattttac aggctcttca
     2761 acattcactt cactttctgg cgtaagaaag gagatatgcc tggccctccc acttgagatt
     2821 gggggcactg gagcaggtc
//
LOCUS       DAOOMC010000205         2797 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdus sp. isolate AMR_MDS_2685
            NODE_44133_length_2797_cov_7.040846, whole genome shotgun sequence.
ACCESSION   DAOOMC010000205 DAOOMC010000000
VERSION     DAOOMC010000205.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864760
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdus sp. (wastewater metagenome)
  ORGANISM  Syntrophorhabdus sp.
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae; Syntrophorhabdus.
REFERENCE   1  (bases 1 to 2797)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2797)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,729
            Genes (coding)                    :: 2,708
            CDSs (with protein)               :: 2,708
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 7 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2797
                     /organism="Syntrophorhabdus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44133_length_2797_cov_7.040846"
                     /isolate="AMR_MDS_2685"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2699756"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..317
                     /locus_tag="PK125_13390"
     CDS             <1..317
                     /locus_tag="PK125_13390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB39137.1"
                     /translation="RRMETSVRARPSQDNQRSYNGHYMKVGMHAEEIVMAWLNEHPIA
                     GGFAPRQSIVKLTGWACLSSCTVDFPNHSTNTSFQSQILAGVFDSHALPPFFFVNSAL
                     PR"
     gene            complement(574..993)
                     /locus_tag="PK125_13395"
     CDS             complement(574..993)
                     /locus_tag="PK125_13395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB39138.1"
                     /translation="MNKKRLFLLLFIFIFLVGCATTHDRLLDTDTSQVQLRNIQSRVF
                     DTTDKDKTLRTVIATLQDLGFIIDKADFSLGSITATKLNQYALRMTVTVQPRGETQLL
                     IRANAQYNITPVVDPEPYQNFFTSLEKSMFLAAQEVD"
     gene            complement(990..1532)
                     /locus_tag="PK125_13400"
     CDS             complement(990..1532)
                     /locus_tag="PK125_13400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB39139.1"
                     /translation="MKFKNSKLAKTLSLFISSLIILSCQPKIPKEALILTPESLENRQ
                     LQTRKYETSDEMKILGACAALLQDLGFGIDESETELGVLVGSKTRSAENAGQIVGAVI
                     LAILIGAYMPTDRDQVMRACIVTKPSEENHCSVRVTFQRIVFNSRNEVSCREFINDPE
                     IYRDFFEKLSKAIFLEAQGV"
     gene            complement(1537..2004)
                     /locus_tag="PK125_13405"
     CDS             complement(1537..2004)
                     /locus_tag="PK125_13405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB39140.1"
                     /translation="MKYSRIIIVFLSLFPLIISGCVNAVNHANEVQQGLDGDKLTVGT
                     VQREIRVGMSGAEVAEVLGSPNIVSTDEKGREVWIYDKIATDKVYSTSSGGISALILG
                     AVIGGTGGVGGGIAPGYSKSAGAASTSQRTLTIIIKYDEQRKVRDFAYHSSRF"
     gene            complement(2478..>2797)
                     /locus_tag="PK125_13410"
     CDS             complement(2478..>2797)
                     /locus_tag="PK125_13410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB39141.1"
                     /translation="RSMEKIVVNAVKKNGKASNPYRDTPPPCEKMQGSRQNPKQTWLF
                     KKENDDRQSCNLRGVTACPARPLKNIRHIVYVGLRDRRMTGVFMPARWSRFIGMLLPG
                     NMP"
BASE COUNT          711 a          656 c          584 g          846 t
ORIGIN      
        1 ggaggcgcat ggaaacgagt gttagggcgc gtccatccca ggacaaccaa cggtcataca
       61 acggtcatta catgaaggtc ggaatgcacg ccgaagagat cgtcatggca tggctgaacg
      121 aacaccctat tgcgggcggt tttgcgccaa gacaatcaat cgtgaagctg acaggctggg
      181 cctgtctgtc ttcctgtact gtggattttc ctaatcattc cacgaatacg tcttttcaat
      241 ctcaaatctt ggcaggtgtg ttcgattccc atgcacttcc gccatttttt ttcgtaaatt
      301 cagcactccc ccgataaaaa tccttttatt taacaggact ttaagcttct ttaacctgcc
      361 aatgctgagc cccacactgg atttgaagtc gtgaagtctt ttccgagaac attgaatacg
      421 taaagaaggc aagtctctgt ctataccttg cccctacccg aaaagtaagc tattttagat
      481 tgtcttttga gaaaaaagtg cagatgaaaa gatttgtcag caagtcaggc tcatcgctta
      541 ttttctaaac agagcctgac ttcaaaaaag atttcagtcc acctcctgtg cggctaagaa
      601 cattgatttc tcaagggatg tgaaaaagtt ctgatagggt tctggatcaa caacgggtgt
      661 tatattatat tgggcattgg ccctgattaa tagttgtgtt tccccccttg gctgaaccgt
      721 aacagtcatt ctcaaggcat attgatttag ttttgtcgct gttatggaac cgagagaaaa
      781 atcagcttta tcaatgataa accctaggtc ttgtagagtt gctattactg ttcgcagagt
      841 cttgtcttta tctgttgtat caaatactct gctttgaatg ttccttaact gaacttggct
      901 cgtatctgtg tcgaggagtc tgtcgtgagt tgttgcacaa ccaacaagga agataaatat
      961 aaacaataat aaaaaaagtc tctttttatt catactcctt gtgcctccaa aaaaatcgct
     1021 ttcgataact tttcaaaaaa atccctgtat atctctggat cattaataaa ttctctacat
     1081 gaaacttcat tccgtgaatt aaatactatc cgttggaacg ttacccgcac actacagtga
     1141 ttttcctcac tgggtttagt aacaatacaa gccctcatga cttggtctct gtctgttggc
     1201 atataggcac cgattagtat agccaaaata acggcaccaa caatctgtcc ggcattttcc
     1261 gcgcttctgg tctttgagcc taccaatacc ccaagttctg tctctgattc atcgataccg
     1321 aatccaagat cttgaagtag cgctgcacaa gcaccgagaa ttttcatttc atccgatgtt
     1381 tcgtattttc tcgtttgtaa ttgtctgttt tcaagtgatt ctggcgttag gatcagtgcc
     1441 tctttaggaa tttttggttg gcatgagaga atgattaatg atgaaatgaa aagtgataga
     1501 gtcttcgcaa gtttgctatt tttgaacttc atgtaattag aacctcgaag aatgataggc
     1561 aaaatctcgc acttttctct gctcatcata tttaatgatt attgtcagtg tgcgctgact
     1621 cgttgatgca gctcctgcgc tctttgaata cccaggggca atgcctcctc ctactccccc
     1681 tgttcctcct atcacggctc cgagaattag cgcagagata ccaccactgc tggtcgaata
     1741 aactttgtca gtggctattt tatcgtaaat ccaaacctct ctaccttttt cgtccgtaga
     1801 gacgatatta ggtgatccaa gtacttctgc aacctctgca cctgacatgc caactctaat
     1861 ttctctctgt acagttccaa ccgtgagttt atctccatcc aaaccttgct gcacttcatt
     1921 tgcatgattt accgcattaa cacaccctga aatgatcagt gggaataacg aaagaaatac
     1981 gatgataatt cttgaatatt tcatggaccc cccctagaaa taatctgaaa ttttaattgc
     2041 acatacttct tcgattgtca atgcctaaaa aacactgaac cgccccgtgg tttccggagc
     2101 ctctgtcatt tgatcccggc aaccacggcg ggggtatcct gatacggatg ctggatagat
     2161 cttgccgaag ctagatggcg agataatgac gacggacatc gtctcggatg ataatcgtca
     2221 ttacttcctt tcgagccgta atagctgatc ctgtgggttg gccggtgctg cgacttcgta
     2281 ggtgttaccg agaagtctgc aatgttccag ccgtcttttt ccaggagttg aaaatacagt
     2341 aagggattcc aatcaacctg gatggggccg gacgacgatc catctggccc ctgccaaccc
     2401 tccacctcgt ccgcttcatc acataccatg cctggttcgt cacatttgcc ttgcccggcc
     2461 ttataaaagt gttgttttca aggcatgttc ccgggaagaa gcattccgat gaatcgagac
     2521 caccttgcag gcataaaaac accggtcatg cggcgatctc gaaggccgac ataaacgatg
     2581 tgacgaatgt tttttaatgg ccttgccggg catgcggtca ctccccgcag gttacagctt
     2641 tgtcggtcgt cgttttcttt cttgaaaagc caagtttgct tggggttttg ccggcttcct
     2701 tgcattttct cgcagggggg aggggtgtct cggtaggggt tcgatgcctt gccatttttt
     2761 ttaacagcat tgactacgat tttttccatt gatcgcg
//
LOCUS       DAOOMC010000206         2770 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdus sp. isolate AMR_MDS_2685
            NODE_44741_length_2770_cov_3.405525, whole genome shotgun sequence.
ACCESSION   DAOOMC010000206 DAOOMC010000000
VERSION     DAOOMC010000206.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864760
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdus sp. (wastewater metagenome)
  ORGANISM  Syntrophorhabdus sp.
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae; Syntrophorhabdus.
REFERENCE   1  (bases 1 to 2770)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2770)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,729
            Genes (coding)                    :: 2,708
            CDSs (with protein)               :: 2,708
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 7 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2770
                     /organism="Syntrophorhabdus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44741_length_2770_cov_3.405525"
                     /isolate="AMR_MDS_2685"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2699756"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            248..1066
                     /locus_tag="PK125_13415"
     CDS             248..1066
                     /locus_tag="PK125_13415"
                     /inference="COORDINATES: protein motif:HMM:NF021254.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exosortase/archaeosortase family protein"
                     /protein_id="HPB39142.1"
                     /translation="MVKFSVLVALWIVSFIPVFPRLVYTWLNHADNSHGILVPLISLF
                     FVWQKRGDLKAAKISSSNWGAFILSASMFLYLLGLASGTEIISRTMVVFSLIGLLLFN
                     LGKEVVKILFFPLLFLLFMIPVPIALQSAVAFPLQLFATKISFFFIQALSIPVYQEGN
                     MLYFAHAQLEVAEACSGIRSISAFTVLSVVFAYLLDKGWSRRIVLLASAIPLAMFTNI
                     IRITGTGILAHFYGSRIADSFLHEFSGIVVFAFGFILFLFEFSLLNRVRSRKSN"
     gene            1123..1773
                     /locus_tag="PK125_13420"
     CDS             1123..1773
                     /locus_tag="PK125_13420"
                     /inference="COORDINATES: protein motif:HMM:NF023410.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EpsI family protein"
                     /protein_id="HPB39143.1"
                     /translation="MSKKSFTISLVVLLATIGLVALIAKRGSPVVVQTNLEKIPMNIA
                     GYSATEDAFPDYVYEALNADQHIYRHYKGVDGREIDLYIGYYGTAKGGRTGHNPHGCL
                     PGAGWVILKTHKIRVIPSYYPDGVDLNYILTRKGDAFICLIHWYQSAGKKVLSTGFEQ
                     NIERFKGRILHNRNDGAFIEVYTLTTKNEIEEAKLLVRNFSEKLLDLLPDYWPVEE"
     gene            1951..2193
                     /locus_tag="PK125_13425"
     CDS             1951..2193
                     /locus_tag="PK125_13425"
                     /inference="COORDINATES: protein motif:HMM:TIGR02595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HPB39144.1"
                     /translation="MKKLLLRGFIIGASMIVLSGLISSVDAMGSLPGGGGHGGGHYGG
                     GGGRPTPAPEPSTLALLGAGAAGAGAYFIAKIRKKK"
BASE COUNT          803 a          466 c          689 g          812 t
ORIGIN      
        1 attaccttgg cctttttggt catatagtgg ggatatacca tggtcctcga caccttccgt
       61 tcatggtcgg acgaaggaaa attttgacaa ccgcatatgg actgtctttg aaagattaat
      121 aactgttctc ttatttactc aatgttttta taagactgaa atcctttctt agaagacaat
      181 caattttgat atcgtagaaa tgaaagaatc aagcacatcg ctttatcgtt tacccaagaa
      241 ctctcttatg gtaaagttct ctgtactcgt tgccctctgg atcgtgagtt tcataccggt
      301 ttttccgcgt cttgtatata cctggttaaa tcatgcagat aactcccatg gaatcttagt
      361 tccgttaatc tcgttattct tcgtttggca gaagaggggc gacctcaagg cggctaagat
      421 ttccagttca aattggggtg cctttatttt atctgccagt atgttcttgt atctgcttgg
      481 tcttgcaagc gggacagaaa tcatatcacg taccatggtt gttttttctc tcatcgggtt
      541 gctgcttttt aatctgggaa aagaagtggt gaagattttg ttctttcccc ttctgttttt
      601 gctgtttatg atcccggtgc ctatagcatt gcaaagcgca gttgctttcc cgctgcaact
      661 gtttgctaca aaaatttcct ttttttttat ccaggctctt tctatccccg tttaccagga
      721 agggaacatg ctgtattttg cccatgctca gttggaagta gctgaggctt gtagtggtat
      781 acgttctatt tctgcattta ccgttttgag tgttgttttt gcctacttgc tggacaaagg
      841 atggagtcga agaattgtgc ttttggcttc agctattccc ctggcgatgt tcacaaatat
      901 tattcgtatc accggcacag ggattcttgc tcatttctac ggatcaagaa ttgcggacag
      961 tttcttgcac gaattctcag gcattgttgt ttttgcgttt gggtttatct tgtttctatt
     1021 tgaatttagt ttgctgaata gggtgaggtc caggaagagc aactgatgat gagccagcag
     1081 tgtccagtca acagttaaga ggagcaatag tttagcaaac atatgagtaa aaaatcgttt
     1141 actatctctc ttgttgtttt actggcaaca atcgggctgg ttgcgttgat tgcaaaacga
     1201 ggttcgcctg ttgttgtgca aaccaacctt gaaaaaattc caatgaacat agcaggttat
     1261 tctgctacgg aggatgcctt ccctgactat gtttatgaag cattgaatgc cgatcagcat
     1321 atttatcgcc attataaggg tgtcgatgga agggaaattg atctatacat tggctactat
     1381 ggaactgcga agggtgggag gactgggcac aatccacatg gatgtctacc tggggcaggt
     1441 tgggttattc ttaaaaccca taagatacgt gttataccaa gttactatcc tgacggcgtt
     1501 gaccttaatt atatcctgac ccgcaaaggg gacgcgttta tatgtttaat acattggtat
     1561 caatcagctg gaaagaaagt cttatcgaca ggttttgagc aaaatattga gaggtttaaa
     1621 gggagaattt tgcataatcg aaatgatggc gcgtttatag aggtctatac tttaacgaca
     1681 aaaaatgaaa tagaagaagc aaaattgttg gtaaggaatt tctcggagaa gctgttagat
     1741 ttgttacctg attactggcc tgttgaagaa taagggaaga tgtatgtagg tgctgaatac
     1801 acaagcttac acaaaagaga aataaatatg gatcaaatcg atttaatagg ttgagtatca
     1861 ggaaataagc aactataatg atacagtgag aatttgccga taatatatag tacgtcacag
     1921 aataaggaca atatttaatg gaggttcgtt atgaagaagt tgctgttaag aggttttatt
     1981 attggggcgt caatgattgt actttcgggg ctaatatcat ctgttgacgc gatgggttcg
     2041 ttacctggag gaggaggcca tgggggtggc cattatggtg gcggtggtgg tagaccgact
     2101 ccagcaccag agccatcaac ccttgcgctc ttaggagcag gtgctgcggg agcaggcgca
     2161 tacttcattg cgaagataag gaagaagaaa tgaaaaatat agtctgtcga gtcattgagt
     2221 ctattgggtc atagagtcca tagggtctgt tgggtcatag ggtcggtaaa tcagctcaaa
     2281 gctcaaagct gatagcaaag agttcatagg gacaaggtaa aaggtgggag gtaggaggca
     2341 aaagggaaag agaggcagct catagctgta agctcatagc aaagagaaag gcgaagagct
     2401 catagcttat agctgtaagc tgaaagcaaa gaataaaaaa ggcggaagga aaaaggtggg
     2461 aggtaagagg taggaggcag gggggaagaa aaaggtaata gctgtaagct gtaagctcat
     2521 agcgaagaga aaggcaaaaa gctgatagca gagaataaaa aaggtgaaag ggaagaggta
     2581 ggaaggtagg agggaaagaa aaagctcata gctctaagct tatagcgaag agctcataaa
     2641 gacaaggcaa aaggtaagag ggaagtctga ttgggaataa tagttcctat cctcgatgcc
     2701 agttctccca tttcagtcca gcaaaaacta tataatcatt gaagtttctg gtgatcagcg
     2761 ttaagctatt
//
LOCUS       DAOOMC010000207         2730 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdus sp. isolate AMR_MDS_2685
            NODE_45613_length_2730_cov_5.391776, whole genome shotgun sequence.
ACCESSION   DAOOMC010000207 DAOOMC010000000
VERSION     DAOOMC010000207.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864760
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdus sp. (wastewater metagenome)
  ORGANISM  Syntrophorhabdus sp.
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae; Syntrophorhabdus.
REFERENCE   1  (bases 1 to 2730)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2730)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,729
            Genes (coding)                    :: 2,708
            CDSs (with protein)               :: 2,708
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 7 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2730
                     /organism="Syntrophorhabdus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45613_length_2730_cov_5.391776"
                     /isolate="AMR_MDS_2685"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2699756"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            98..1252
                     /locus_tag="PK125_13430"
     CDS             98..1252
                     /locus_tag="PK125_13430"
                     /inference="COORDINATES: protein motif:HMM:NF012318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPB39145.1"
                     /translation="MAREDGVIITNYHVVSMATDIKIKIGQRTKNVEGLLHVDIENDI
                     AVIKVEGDNYPRVTLGDASKALVGEKVYVIGSPQGLENTISEGILSGIREIDQGKKIL
                     QMTAAISPGSSGGPVFNSRGEVIGIATFLIAETQNLNFAMPVNLAMAGLSKKQLVSPQ
                     EACKLDFNETASCWLYQGLAYGSAGQHDRAADAFKRSLSIDSKKAETYVNLGISYANM
                     GKYQEAIDVLNQAVAIEPNRAETLSKLGTVYSQMEKYDEAIRNLKKSIDLKPDDPLTH
                     FNLAVTYEKINQYEKAIESAQETIRLAPDFPDAHGFLGFTYTNLNMYAEAAVAYKSAI
                     RLNPDDPRMHLGLGKAYVNLGDRPLALDEYKILMKLNPDMANTLFDLIYK"
     gene            complement(1348..1728)
                     /locus_tag="PK125_13435"
     CDS             complement(1348..1728)
                     /locus_tag="PK125_13435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB39146.1"
                     /translation="MKIIFLIGAALILSATWAFGSPFLVSDPYPREDKGLMKFLVTIN
                     GETKESLPAKNADGKLYLKYDLGSLADGIYTVTIKAVNTKGQESSPATYSFKKTGDRI
                     EPYTPPVPKQKIAPTRSYPGHMSK"
     gene            2192..2422
                     /locus_tag="PK125_13440"
     CDS             2192..2422
                     /locus_tag="PK125_13440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB39147.1"
                     /translation="MVEEAVVPLPEVRELKTKVCEPERILSKKTMEVEILKDAIEIAR
                     EEKLIVRMPIPPHLHVSPIRKACRIPEETHPS"
BASE COUNT          809 a          589 c          628 g          704 t
ORIGIN      
        1 cgatattaca cggtcagcga gaccacctta cagatgttct aatgcatgat ttgggataaa
       61 ccgagaggga aaacccactg gcattggaag cggttttatt gcccgtgaag atggtgtcat
      121 tattacgaat tatcacgtag tcagtatggc tactgacatt aaaattaaaa ttggccagag
      181 aacaaagaat gttgaggggc ttctccacgt ggacattgag aacgatatag ccgtaatcaa
      241 ggttgaaggg gataattatc ccagggttac gttaggagat gcaagtaagg cactggtggg
      301 agaaaaggtt tacgttattg gaagccccca ggggctagaa aacacgatct cagaaggcat
      361 cctcagcggc atccgtgaaa tcgaccaggg taaaaagatc ctccagatga ccgctgcaat
      421 atctcctggt tcgagtggcg gaccggtatt taacagccgg ggcgaagtta tcggtatagc
      481 aacatttctg attgcagaga cacagaatct taactttgca atgcctgtga atctcgctat
      541 ggccgggctt tcgaaaaaac agcttgtcag tccccaggaa gcatgtaagc ttgattttaa
      601 cgaaactgct tcttgctggc tgtaccaggg gttagcatat ggttcagcag gacagcatga
      661 cagggctgca gatgcattca aacgttcgct ctccattgat tcaaaaaaag cagagaccta
      721 tgtaaatctc gggattagct atgcaaatat gggtaaatac caggaggcaa ttgatgtcct
      781 caaccaagct gtggcaattg aaccaaatcg tgcagagacc ttgagtaaac tgggtactgt
      841 ttatagccaa atggaaaaat acgatgaagc tatcaggaac ttgaaaaaat ctatcgacct
      901 taagccagat gaccccctta cacattttaa ccttgccgtc acttacgaaa aaataaatca
      961 gtatgaaaag gctattgaat cagcccaaga aactatcagg ttggcacccg attttcctga
     1021 cgcccacggt tttcttggtt tcacatacac gaatttgaat atgtacgccg aagcagcagt
     1081 agcatacaag tcagccatac gattaaaccc tgacgatcca cgaatgcatc ttggcctcgg
     1141 aaaagcatat gtaaatctcg gcgataggcc tttggcactt gacgaataca aaattctgat
     1201 gaaactgaat cctgacatgg caaataccct gtttgatctt atctacaaat aattgggact
     1261 gattttatcg atctgatttc ccctttaatc tttgattttt ttctgtagtt gccgtgagtt
     1321 tacagcaatg agttgaatac ctgtagctta cttgctcata tgccccggat agcttctggt
     1381 gggggcgatt ttttgtttcg gaacaggtgg ggtgtatggc tctatcctgt ctcctgtttt
     1441 cttgaaagag taagttgctg gagaggattc ctgacctttt gtattcaccg cttttattgt
     1501 aacagtatat attccatctg caagactccc aagatcgtat ttcaggtaga gctttccatc
     1561 tgcatttttt gccggcagtg actcctttgt ttccccatta attgtaacaa gaaatttcat
     1621 caatccttta tcttctcttg gataaggatc gcttacaaga aagggagatc cgaatgccca
     1681 tgttgctgag agtattaaag cagcgccaat aagaaaaata attttcattt ccagtaccct
     1741 ctattttccg gtatttttta aagcattgca atggcaaatt aaacaaatcg caagatgcag
     1801 aagcattcta tcacgcctat atggatcaag aaagtaaaaa ttgctttctc taaaaatatg
     1861 atgaccgcat acactggcct tagtcgcatg gattcctttt tcaaatatat atgggtacag
     1921 gaggcaatag agctgttcag gagcattctc ccaacagtat gagcgtatac agcaagataa
     1981 tcatgtacat ttccatagcg tatgccagta cagatagatt tgtccatata gtgtagtagc
     2041 ccctgtttag accggacact catacaacca aaataagttg tagtaataac cctggagtta
     2101 ttcctataat caaggaaagg aggcctatga gcccggtaga agccatgacg ttaagagaag
     2161 aaggcgctgg agcgcagcac agaaaagggc gatggttgaa gaggcagttg ttcccctccc
     2221 ggaggtcagg gaactgaaaa ccaaggtctg tgagccagag cgtatcctga gtaagaagac
     2281 catggaagtt gaaatcttaa aggatgccat cgagattgcc cgtgaagaaa aactgatcgt
     2341 gcggatgccc atacccccac acctgcatgt atcgccgata cgaaaagcct gcaggatccc
     2401 ggaggagacc cacccatcct gatgatggaa tagagtgtgc ccttatcggg ataattgccg
     2461 gcgaccgccc tacctgcgga taccggagag tcactgccct tctcaacagg atgcctggtg
     2521 aacaaggaca acccagggtg aaccataagc gtatctatgg gatcatgaag atgaatcatc
     2581 tcttactcca gaagacatac gggaagaccc ataggatccc acgaagggac aaacatcact
     2641 cttaaacagc aacatgcact ggtgctccca tgtcttcgag attgcctgcg agaacaggga
     2701 acggatcagg gttgtctttg ccatggactg
//
LOCUS       DAOOMC010000208         2724 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdus sp. isolate AMR_MDS_2685
            NODE_45748_length_2724_cov_6.049457, whole genome shotgun sequence.
ACCESSION   DAOOMC010000208 DAOOMC010000000
VERSION     DAOOMC010000208.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864760
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdus sp. (wastewater metagenome)
  ORGANISM  Syntrophorhabdus sp.
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae; Syntrophorhabdus.
REFERENCE   1  (bases 1 to 2724)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2724)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,729
            Genes (coding)                    :: 2,708
            CDSs (with protein)               :: 2,708
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 7 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2724
                     /organism="Syntrophorhabdus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45748_length_2724_cov_6.049457"
                     /isolate="AMR_MDS_2685"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2699756"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..408
                     /locus_tag="PK125_13445"
     CDS             <1..408
                     /locus_tag="PK125_13445"
                     /EC_number="1.-.-.-"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: protein motif:HMM:NF013412.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine-phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HPB39148.1"
                     /translation="TEQQIKQQEVVTMIPEKLLEILKQDGVVAIATLGKDGPHMVNTW
                     NSYIRISSDERLFIPAGYMHKTEANVAFNPNVLITLGSSKVQGLRGPGTGFLIKGKAS
                     FITSGPDYDFMKERFSWLRATLAITIESATQTL"
     gene            1164..1841
                     /locus_tag="PK125_13450"
     CDS             1164..1841
                     /locus_tag="PK125_13450"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HPB39149.1"
                     /translation="MNTNNYYDKKLSSLLLAEVYSTEIPRINQYLQAEIEFVRKHIRG
                     DETILELGAGYGRVILELARSCKKIVGIDISPNNIEYGKKLLKDYKNAELVVMDAYTM
                     QYKNEFDMTICIQNGLSAIKGDTRDLLKKTIDGLKNGGLAFFSSYHPDFWEYRLLWFY
                     EQAKKGLIGELDIENCRDGVITCKDGFYSRSFSKDELSELGQQCGMNYWIEEIDESSI
                     FLIIQKL"
     gene            2643..>2724
                     /locus_tag="PK125_13455"
     CDS             2643..>2724
                     /locus_tag="PK125_13455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_254131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HPB39150.1"
                     /translation="MSAVFAALTAIFAKIGLEGVDSDLATL"
BASE COUNT          869 a          515 c          639 g          701 t
ORIGIN      
        1 acagaacaac aaatcaaaca acaggaggta gtcacaatga tcccggaaaa actgctggaa
       61 atcctgaaac aggatggagt tgttgccatt gccacccttg gaaaagatgg gcctcatatg
      121 gtcaacacct ggaatagtta tattcgaata tcatcagacg aacgcttgtt cattcctgcc
      181 gggtacatgc ataaaacaga ggctaacgtt gctttcaacc ccaatgtgct gattacactt
      241 ggaagcagca aggttcaggg cttgcgtggg cccggtaccg gcttcctcat caaagggaag
      301 gcatctttta ttacgtcagg acctgattat gacttcatga aggaaaggtt cagttggctg
      361 cgagccactt tggccattac tatcgaatct gctactcaaa ctttatgatg gtgacagacc
      421 agtctgcata taacggtttc atcgatttgg caagcatggg acacgtgtca agtacgaact
      481 gtccaacaat gacctaaagg cgatgcggta acccgcgcgc ttaagcacag acgttagacg
      541 cggattcaat atgttcttca ataacacaaa cacacctgac agacctgtga gggtgggttg
      601 tcaaccggga attcctatcc attgcatatg cagcaaaaag aaggtggaac tcttctggtc
      661 tagaccagcc tgtggtggcc cgtatccggc aatcttgttt atttacggcc atcaaaaacg
      721 ggtaagggac ggaggcgaaa gattttaatg ttttcgctat ctatattaac gagcttaacg
      781 cagtgccaga gaagataccc acggcaagcc cgtggcactt aaatatactt actgtgggtg
      841 aatgaataag tacattcatc agccaacccc tgaaagtcgt ggtcctctta tgtggcttac
      901 ctctttcatg gaaagctata cagcgaatac ttgagcttga agggagggat ggcagcgcta
      961 acagttttac tcgtggcgag agcgatgcaa gctactgcaa tagaggaacc aaatgattgg
     1021 aagatcggtc gtagtaaacg taaaaatgga ttttgttcag tccagcggag gtggagaaac
     1081 cacgctgctc aagcgtatcg ttatttgggt gctacgcgca caacatgtaa tttgctgtgc
     1141 gagttttgcc agaggcggtg actatgaata ccaataacta ctacgacaaa aagttaagct
     1201 cgttactgtt ggcggaggta tattcaacag aaataccgag gattaatcaa tacttacagg
     1261 cagaaatcga atttgtcagg aaacacattc gcggagatga aaccatatta gaacttggtg
     1321 caggatatgg aagggtcatc ctggaattgg ccagatcatg caaaaagata gttggtatag
     1381 atatatcgcc taacaatatt gagtatggta aaaagctttt aaaggattat aagaatgcag
     1441 aactagttgt aatggacgca tatacaatgc agtataaaaa cgaatttgat atgacaatat
     1501 gcattcagaa tggtctttca gcgataaaag gggatacacg tgatttgctg aaaaaaacta
     1561 tcgatggttt aaaaaatgga gggttagcat tttttagttc atatcatcct gatttctggg
     1621 aatatcgtct actgtggttt tatgaacagg caaaaaaggg attaatagga gaactcgaca
     1681 tcgagaactg cagggatgga gtaataactt gtaaagatgg attctactct cgatcttttt
     1741 caaaagatga attatcagaa cttgggcaac aatgtgggat gaattattgg attgaagaaa
     1801 tagatgaatc cagcattttt ttgataatac aaaagttata gtcgaatttc cacgtaacaa
     1861 tcgtttttat catgcttttt gcaaaattga atactaaaag tacttgctaa agggaaaaga
     1921 atcgactgcc agtggcaagt atgttacata aagaaaaaag ggggaataaa ccatccctta
     1981 tttgaagata gtatgatatc atatcaaact atatagggga tggcgttaca ggcaaagaga
     2041 gtctagtgat tttgctggat gaagaattca tcgatgagtt tgacaggcat gtgaatgaac
     2101 aagcctgtca aggcagaagc acacgattcg ggaagccgtc tctgagaagc ttttgagact
     2161 gaagagcaac tgtttagcat cagaatgcgc aaaactcgtt gctgcatttg aaaaaaagat
     2221 agctaaaaaa ggactggata aggatgtaaa tcagtgacca caatgctgag atggaaaatc
     2281 agatgtgcag attttaatcc gaccagggtc agagaacagt ctggcttacg cccagttctg
     2341 acattcagtt atgatgtctc caatgaaaga tcgggtacgg taataaccct gatgttaacg
     2401 aatcaatcgc agaaagcagg attcccactc atacttaaac tgaaacccaa aaatctatcg
     2461 gagcagtcat ggttaaaggt gagtcaaatc aggaccctgt cggtggacag gattagaaat
     2521 ctaaatgaaa tatcgcataa ccaacagccg cagttttctc acaactctgc ggttcaacta
     2581 cgtgttagac actactgagg acaactgtgg ccacttctca cacctggttc ttctgggcag
     2641 ccatgtcagc cgtatttgcg gctctgactg caatttttgc taagattggc ctggaaggtg
     2701 tagactctga tttggcaaca ctaa
//
LOCUS       DAOOMC010000209         2699 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdus sp. isolate AMR_MDS_2685
            NODE_46345_length_2699_cov_4.645613, whole genome shotgun sequence.
ACCESSION   DAOOMC010000209 DAOOMC010000000
VERSION     DAOOMC010000209.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864760
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdus sp. (wastewater metagenome)
  ORGANISM  Syntrophorhabdus sp.
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae; Syntrophorhabdus.
REFERENCE   1  (bases 1 to 2699)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2699)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,729
            Genes (coding)                    :: 2,708
            CDSs (with protein)               :: 2,708
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 7 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2699
                     /organism="Syntrophorhabdus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46345_length_2699_cov_4.645613"
                     /isolate="AMR_MDS_2685"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2699756"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            291..1808
                     /gene="istA"
                     /locus_tag="PK125_13460"
     CDS             291..1808
                     /gene="istA"
                     /locus_tag="PK125_13460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011937498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HPB39151.1"
                     /translation="MKKLKEIARLLLECNLGIRPIARACNISTSTASTYVEKFKELGA
                     TYKEICETDEDTLLELLFTKKESSKQLPDFPYLVGEMKKKGVTLQLLYEEYKRDNPDG
                     YERSRFYQLYREWVKKADPVMRFTHKAGEKMFVDFSGDKPHYQNPATGERVEVELFVS
                     ALGASSYIFAYAVPDQTTESFTKCNIKAFEFYGGCSQCLILDNLKAGVTHACYYDPEI
                     NKTLAAMAQHYNIAVIPTRIAKPKDKAKVENAVLQAQRRILATLRNRTFFSLIELNEA
                     IAEETKRLNERPMAAINKSRHDLFIEIEKPLLKPLPEERFVIASWTKAKVHIDYHVKV
                     EKIYYSVPYTLIGETVDIRYTGDVVEIYHKGKRVASHIKTNKSGTFVTQNLHMPTEHR
                     QYLEWTPARIKLWGQKIGPNTRTLMEEIMNHRVHPEQGFRSCLGIIRLSKAYSPERVE
                     NACKRVLEIGAYNYKSVKSLLQMGLEGFTTDVENKKIVPLHNNIRGNEYYRGGNS"
     gene            1805..2548
                     /gene="istB"
                     /locus_tag="PK125_13465"
     CDS             1805..2548
                     /gene="istB"
                     /locus_tag="PK125_13465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003290589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element helper ATPase IstB"
                     /protein_id="HPB39152.1"
                     /translation="MIEATIEKLIAMKLSGMAEGLQEQISNHAYKDLSFEERLGILVD
                     KEKLSRENRQLKILLSHAHLRHHNACFEDIDFRTRRGLSKDVILKLSQNEWIRSNQNI
                     IIVGPTGSGKTYIACSLGNSAVRQGIQTVYIRLPRLAQEMKIARADGSFVKFLGRLQR
                     IRLLIIDDWGINPFTDDERRNFLEIMEDRYNVRSTIIASQFPVDTWHDIIGEPTLADA
                     ICDRIVHNAHKIILKGGEDSMRKIYSNLT"
BASE COUNT          956 a          489 c          578 g          676 t
ORIGIN      
        1 caggaaggta tccatcatga tcgaggagaa gatggagttt gaccgcacct ttggtgtaaa
       61 atccactcaa cctcgaacac tgaatccgat aaaggtcgaa caccaaatcc gataaaggtc
      121 gaacaccaaa tccgataaag gtcgaacact tttttcgttt tattggattt tctgttcagg
      181 taaaaacata tcaacgttta caatgattcc ctccctgtcc tatatgatac actaccatca
      241 aaaagtagat ggagggtgtg taagtgaaaa agaaagggag gaaaacatca atgaaaaaac
      301 taaaagaaat tgcccgatta ctactggaat gcaatcttgg tatacgtcct attgccaggg
      361 catgcaatat atccacttca acagcttcaa cctatgtgga gaagtttaaa gaactcggtg
      421 ctacttataa agagatctgt gaaacagatg aagacacact acttgaactt ctattcacaa
      481 aaaaagaatc atcaaaacaa cttcctgatt ttccctatct tgtgggggaa atgaagaaaa
      541 agggggttac ccttcagctt ctctatgagg aatataaaag ggataatccc gatggatatg
      601 aaagaagcag gttttatcaa ctctatcgtg aatgggtcaa aaaggcagac cctgttatga
      661 ggtttaccca taaggcagga gaaaagatgt ttgttgactt ctcaggggat aaacctcatt
      721 atcagaatcc tgcaacagga gaaagggtag aggttgaatt atttgtctct gcattagggg
      781 caagctcata tatatttgca tatgccgtac cagatcagac aacagaaagc tttacaaaat
      841 gcaatataaa ggcatttgag ttttatggag gatgttcaca atgtctgatc ttggataacc
      901 ttaaagccgg tgtgacccat gcatgctact acgatcctga gatcaacaaa accctggctg
      961 ccatggcaca gcattataat atcgccgtta tccccacaag gattgctaaa ccaaaggata
     1021 aggccaaggt agaaaacgcc gttttacagg cacaaagaag aatccttgca acactcagaa
     1081 acagaacctt ctttagtctt atagagctga acgaagctat tgcggaagaa acgaaaagat
     1141 taaatgaaag accgatggca gcaataaata aatcccgtca tgatctattt attgaaattg
     1201 agaagcctct tttaaaaccc ctgccggaag aaaggtttgt cattgcttca tggacaaagg
     1261 caaaggttca catcgattac catgtaaagg tagaaaaaat atattatagc gttccctata
     1321 cattgatcgg ggaaactgtt gatataagat ataccggcga tgttgttgag atataccata
     1381 aaggcaaaag agttgcctct cacataaaaa caaataaatc cggcacattt gttacacaaa
     1441 accttcacat gcccacagaa cacaggcagt atcttgaatg gaccccggcg aggataaaat
     1501 tatggggaca gaagatagga ccgaatacaa gaaccctgat ggaagaaatc atgaaccaca
     1561 gggtacaccc tgaacaaggc tttcgaagtt gtcttggcat tataagacta tcaaaggcat
     1621 attctcccga aagggtagaa aacgcatgta aaagggtgct tgaaataggg gcatataatt
     1681 acaaaagcgt aaagtcatta cttcagatgg gattagaggg ttttacaaca gatgttgaaa
     1741 ataaaaagat tgttcccctt cacaacaaca taagaggtaa tgaatactac cggggaggca
     1801 actcatgatt gaggcgacca ttgaaaagct tattgccatg aagctgtccg gtatggcaga
     1861 agggttacag gagcagatat cgaatcatgc atataaagac ttaagttttg aggaacgtct
     1921 gggtatcctt gtcgataagg aaaaactctc aagagaaaat cgtcaattga aaatccttct
     1981 ctctcatgca catttaagac atcacaatgc ctgttttgag gatatcgact tcagaacaag
     2041 gagggggtta tcaaaagatg taatccttaa attatctcag aatgaatgga taagatccaa
     2101 tcagaatatc ataattgtcg gacctaccgg atctgggaaa acatatattg cttgttcact
     2161 tggtaacagt gctgtcagac aaggtataca aaccgtctat ataagattgc caagattagc
     2221 ccaggagatg aaaatagcaa gggctgacgg gagctttgta aagttcttag gtagactcca
     2281 gaggataagg ctccttatca tcgatgactg ggggataaat ccatttaccg atgatgaaag
     2341 aaggaacttc cttgagatca tggaggacag atataatgta aggtcaacaa tcattgcaag
     2401 tcagttcccg gtagatacat ggcatgacat cataggggaa cctacccttg cagatgcaat
     2461 atgcgacagg atagtccata atgcccataa gatcatatta aaaggaggag aagattctat
     2521 gagaaaaata tattcaaact tgacttagat cgaacacttt tgtaaagtag agacggcagg
     2581 gaaagaatca taaaaaggcc gttcggataa aatcggattt agtgttcgaa tagattggat
     2641 tatatatttt cattaatata aaaaaagggg cttgcttttt tacatagagg aatgacagt
//
LOCUS       DAOOMC010000210         2690 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdus sp. isolate AMR_MDS_2685
            NODE_46542_length_2690_cov_3.590133, whole genome shotgun sequence.
ACCESSION   DAOOMC010000210 DAOOMC010000000
VERSION     DAOOMC010000210.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864760
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdus sp. (wastewater metagenome)
  ORGANISM  Syntrophorhabdus sp.
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae; Syntrophorhabdus.
REFERENCE   1  (bases 1 to 2690)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2690)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,729
            Genes (coding)                    :: 2,708
            CDSs (with protein)               :: 2,708
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 7 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2690
                     /organism="Syntrophorhabdus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46542_length_2690_cov_3.590133"
                     /isolate="AMR_MDS_2685"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2699756"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            874..1161
                     /locus_tag="PK125_13470"
     CDS             874..1161
                     /locus_tag="PK125_13470"
                     /inference="COORDINATES: protein motif:HMM:NF020250.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HPB39153.1"
                     /translation="MEKKTKAIVLNQKDNVATAIVALEAGTTVVFVKEGYAEKVKLVS
                     DIPMGHKFAIVNIEKGCAVIKYGEPIGESTRQIGRGEHVHVQNLVSKRGNG"
     gene            1158..2327
                     /locus_tag="PK125_13475"
     CDS             1158..2327
                     /locus_tag="PK125_13475"
                     /inference="COORDINATES: protein motif:HMM:NF016203.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HPB39154.1"
                     /translation="MRDTIKFMGFNRPDGKAGTRNHILVIPSVGCSQNAARAIAHGLK
                     GVVYLPNILGCGQMGDDSILVKKTLVGFGTNPNVFAVLIVGNGCETLAPQELADGITS
                     HGKRVEVITIQDVGGTKKTISLGRKIVKDMLENAVKLKREPISLSKIILGTECGGSDY
                     SSGLASNPAVGVACDLLVAEGGTVILSETPELIGAEHIFARRARTPEIERQVLDAVTW
                     WENRAIAAGQDIRDGNPSPGNIAGGITTLEEKSLGCIYKAGTSTLEEALTYASHPTKS
                     GLVYMDTPAHDIEQLTGMVAGGAQIVLFTTGRGTPCGSPIAPVIKITANRNTFLKMRD
                     NTDMDTSKIIQGKETIVSAGKRIFEEMISVSSGKMTKAEKLGQSDFSIFQMNPNI"
BASE COUNT          802 a          526 c          680 g          682 t
ORIGIN      
        1 cattccgccc attcaactca actgcactgc agttgggagt acgtgacgca gcagaaaagg
       61 gaggtgggct aagaaaccgc atcagggaca gccattgtga agggaaatac gggaaagtcc
      121 tgtgggggct tatgctacag gtgcgtgaag ggtaactgca gtttttgggt taattaaggc
      181 gtttgctttt catgcacgta tgcattccag gtaaaagttt cgatccgctg cctatcaagg
      241 tgattttgaa attgcctgcc accatagtac acatcggtat cgcgttctct tattctgtaa
      301 gccctttttc tgtgtgtgat ctgaaccgca atagggacag ttttttgccc atttctcagc
      361 ttcttgagtt ttttgatagt atagatcatt gacagtatac atttagaggc tattattggc
      421 cctgtttttt gagattaagc caaatttgat attaagccga agcgcctgtc ccccgctatc
      481 attttttggg gtataacctg agatcagagg aatatgatgg tgttcttcaa gaattcaata
      541 acgttctcca tgatttcctt ttttctgctc ctttcagcaa aaaggacttg catttttaag
      601 aaattcggag gatattttgt ctggcactaa tacggtttaa cttggtgcat taagaagaaa
      661 tcacaggtct acacaaggag aacccatgct tttcagaaca gcgaaataga aggaaagaag
      721 gacaggcata atgggattca atcaggtaat gcaggtttca tcttttgccg ggcttgtaga
      781 aaccaataca acgcacgaaa caacatccgc agcactcgtg ttggcaagaa cagcaatatt
      841 ttttgaaaga acttattgaa taagagattt aaagtggaaa aaaaaacaaa ggccattgtg
      901 ctcaaccaaa aagataatgt agccacggca attgttgccc ttgaagctgg aaccaccgtt
      961 gtctttgtaa aagaggggta tgccgaaaag gtcaaactcg tatccgacat ccccatgggc
     1021 cacaaatttg caatagtgaa cattgaaaaa ggatgtgccg tgatcaagta tggagaacct
     1081 attggcgaga gcacaagaca aattggccgg ggagaacatg tccatgtcca gaatttagta
     1141 agcaaacggg gcaacggatg agagatacga ttaagttcat gggttttaat agaccggatg
     1201 gaaaagcagg cacccggaac cacattttgg tcatcccgag cgttggttgt tctcaaaatg
     1261 cagcaagggc aattgcccat ggattgaagg gtgttgtcta tcttcccaat atcttgggtt
     1321 gtggccagat gggagatgac tccatactgg tcaagaaaac actggttggt tttggtacaa
     1381 acccaaacgt ttttgcagtg ttaatagtgg ggaacgggtg cgaaacgctt gccccccagg
     1441 agcttgcgga tgggattact tctcacggga agcgggtgga agttataaca atccaagatg
     1501 tggggggcac caagaagacg attagcttgg gaagaaaaat cgtaaaagat atgcttgaaa
     1561 atgccgtaaa actgaagcgc gagcctatct ctctcagcaa aattatcctt ggcacggaat
     1621 gcggtgggtc ggattattcc tctggtcttg cttcaaaccc ggctgttggt gttgcctgtg
     1681 atttactggt ggcggaaggt ggcactgtaa tactctcaga aacccctgaa cttataggtg
     1741 cagagcatat ttttgctagg agggcaagaa caccggaaat cgaaagacag gttctcgatg
     1801 cagtaacttg gtgggaaaac agggcaattg cagccgggca agacattcgg gatggcaacc
     1861 cttctccggg taatattgcc ggaggcatta caacacttga agagaaatcc cttggatgca
     1921 tttacaaggc agggacaagc acactcgaag aggccttaac ctacgcatcc caccccacaa
     1981 aaagtggtct tgtgtatatg gatacccctg cccatgatat tgaacagctt accggtatgg
     2041 ttgcaggagg agcacagata gtactcttta ccacaggcag ggggactccc tgtggttcgc
     2101 ccattgcacc ggtcatcaag atcactgcta acagaaacac attccttaag atgagagata
     2161 atactgatat ggatacgagt aagatcatcc agggaaaaga aaccatagta tcagcaggca
     2221 aaaggatttt tgaagaaatg atctcagtat cgtcaggcaa gatgacaaaa gccgagaaac
     2281 ttggtcagag tgacttcagc atattccaga tgaacccgaa tatctgataa aggttggaaa
     2341 tctctgcttc tgtacttgca gaagataata gaccaaccga acaaagaatt gggtagaaaa
     2401 ggtggcggaa agagagcatt ggaagaaggt catgcagcag ggtctagatg actacatcac
     2461 gatgatgaag gaacaggatt tgccggtgga ggagttcctg aaatttgcta ttgtttatac
     2521 ctaaaggagc atcaaaagta accaaattgg aggtaatttt gttgagactc aaagatcaaa
     2581 tttctattgt tacaggtggc ggtaagggta ttggcaaggc catttcgctt gctttcgcca
     2641 aagaaggagc gattgttgta atggcagccc gcagtttgtc cagcatggaa
//
LOCUS       DAOOMC010000211         2642 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdus sp. isolate AMR_MDS_2685
            NODE_47717_length_2642_cov_5.216467, whole genome shotgun sequence.
ACCESSION   DAOOMC010000211 DAOOMC010000000
VERSION     DAOOMC010000211.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864760
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdus sp. (wastewater metagenome)
  ORGANISM  Syntrophorhabdus sp.
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae; Syntrophorhabdus.
REFERENCE   1  (bases 1 to 2642)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2642)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,729
            Genes (coding)                    :: 2,708
            CDSs (with protein)               :: 2,708
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 7 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2642
                     /organism="Syntrophorhabdus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47717_length_2642_cov_5.216467"
                     /isolate="AMR_MDS_2685"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2699756"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(208..726)
                     /locus_tag="PK125_13480"
     CDS             complement(208..726)
                     /locus_tag="PK125_13480"
                     /inference="COORDINATES: protein motif:HMM:NF023276.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3368 domain-containing protein"
                     /protein_id="HPB39155.1"
                     /translation="MNESTVKGRIVSNTGPIIALMIISKLGILRELFDEVVIPHEVHQ
                     ELLQGKSVENFMNLMRITIYEQEKWIKIQDLQTSLNPALKGMLDIGEASVIQLASDIK
                     PDFVLIDERKARKIARSMYGLKVVGTARILVEAKKRGLIQTVKDLLDEMRAGGYWIGD
                     TIVQQILKEAGE"
     gene            complement(723..977)
                     /locus_tag="PK125_13485"
     CDS             complement(723..977)
                     /locus_tag="PK125_13485"
                     /inference="COORDINATES: protein motif:HMM:NF015630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0175 family protein"
                     /protein_id="HPB39156.1"
                     /translation="MKSLSIKYPESVLATLNLSTDDFEKEAKLALAIKLFELGRLTSG
                     QAAQLAEVSRFSFLLSCGRYGAPSVQWDDEELKAEFEDLP"
     gene            complement(1161..2018)
                     /locus_tag="PK125_13490"
     CDS             complement(1161..2018)
                     /locus_tag="PK125_13490"
                     /inference="COORDINATES: protein motif:HMM:NF013921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HPB39157.1"
                     /translation="MARPLRIEYPGALYHITSRGNAGQKIFRSDKDRSVFLQLLGTVI
                     ERFHWLCHAWCFMDNHYHLVIETPEGNLSRGMRQLNGIYTQKYNGKYHKTGHIFQGRF
                     KAILVDKDSYLLELCRYVALNPVRAYMIEKPEDFRWSSYRSTAGFDKAPPFLVVDWLL
                     AQFSSNRKRAESLYREFVLKGITQEAPWRELKGQIFLGDSQFIQRIKTSLPHDLRDIP
                     RSQRFAERPSLSDLAPRLTVPDKTARNKTMYEAHVRYGYTLKEIGDQAGVHYATVSRI
                     IKKMCGADV"
BASE COUNT          699 a          624 c          513 g          806 t
ORIGIN      
        1 accgcagcgt ctcctcgctc ggttgccgca tccttttcgg atggctcctg atgctaagat
       61 tagtttacca tttctggaga tttaatacaa gatcggaact ggctaattcc ttaactttcc
      121 tcaaacttct gtcagctcat cattcagctc actttattgc gggttcacca tttgttgaaa
      181 tgctctattc tgatgaaatt cctacactca ttcaccggcc tcttttagta tttgttgaac
      241 tatggtatcg cctatccaat atcctccggc acgcatttca tccaataaat ccttcactgt
      301 ctgaatcagt cctctttttt tcgcttctac cagaatccgg gctgtaccga caaccttcaa
      361 tccatacatc gatcttgcta tttttcgcgc tttacgctca tcaatcagga caaaatcggg
      421 ctttatatct gaagcaagct ggatgacgga agcttcgcct atatccagca taccttttaa
      481 agcaggattc agtgaggtct ggagatcttg gatttttatc catttctctt gttcatatat
      541 ggtaattcgc atcaggttca taaagttctc aactgacttt ccttgaagca attcctgatg
      601 gacttcgtgg ggaatgacaa cttcatcgaa aagctctcta aggataccca gtttactgat
      661 gatcattaag gctatgatag gacctgtatt ggaaactata cggcctttca ccgtggattc
      721 attcatggta aatcctcaaa ctcagccttc agttcttcgt catcccattg aactgaagga
      781 gcgccatagc gaccacaact caacaaaaac gagaaacgag aaacttcagc aagttgcgcc
      841 gcctggccag aggtcaggcg gcccaactca aaaagcttta tcgccaaagc aagttttgct
      901 tctttttcga aatcatcagt actcaagttc agcgttgcca gcactgactc aggatacttt
      961 attgacaagc ttttcattac aacctcccta agctaataaa atgtttaata taatagcatt
     1021 tttatatgac tgttgcacgc tatttgccca attttttgtt ttacactttc ttatgccaga
     1081 caataatttt gtgtaaactg ataggccaga gttacatgag acgctgtaaa aggggtcacc
     1141 tctggggtca ggtcttgcgt tcacacatca gccccgcaca tcttctttat tatcctgctc
     1201 acggtggcgt aatgaacgcc tgcttgatct ccaatctcct tcaaggtata cccataacgc
     1261 acgtgggctt catacatggt tttgtttctc gctgtcttat cgggaacggt cagcctgggg
     1321 gcaagatcgg agagagaggg tctttctgca aatcgttgag atcgggggat atctctgagg
     1381 tcatggggca gagatgtttt aatcctctgg atgaattgag aatctcccag gaagatctgt
     1441 ccttttaatt ccctccaggg ggcttcctgt gttatccctt ttaatacgaa ctctctataa
     1501 agcgactctg cccttttcct gttcgaactg aactgagcaa ggagccagtc cacaacaagg
     1561 aagggtggag ctttgtcgaa accggcagtt gatcgataac tgctccaccg aaagtcctct
     1621 ggtttctcaa tcatataagc ccgtaccggg ttcaatgcta cataccggca gagctcaagg
     1681 aggtaactgt ccttatctac aagaatagct ttgaatctac cttggaatat gtgccctgtt
     1741 ttgtgatact tcccgttata tttctgcgtg tatatgccat tgagttgcct catgccccgc
     1801 gaaagatttc cttccggagt ctcaataaca aggtggtaat ggttgtccat aaagcaccag
     1861 gcatgacaga gccaatgaaa ccgttcaata actgtaccga ggagttggag gaatacggaa
     1921 cggtccttat cagagcggaa tatcttttgt cctgcattcc cccgggaggt tatgtgatac
     1981 agggctccgg gatattcaat ccgtagagga cgggccatag gcaaagcata tcatgataaa
     2041 tgttgcaata caagacctga ccccgaattc ccaagacctg accccgaatt cctttgcgag
     2101 gttatacgcc tgtttaaact gttgccctct ttgtcttgct ggatatcttt catatatctc
     2161 ccttactgat cggcttaatt ttgagatctc tgccgctaca ctttcaatat ctcttaaaag
     2221 tgttttatag ctcggaacta ttgttttcca acagtagtga aacaggaggg gtattaataa
     2281 acacccaagc atgcttagca ataggataag gcccttgttg taaaagttcc aggttagcca
     2341 tacaacggta accacaagca acaaagaagg gacaaaggtt gcccaaaact tcttcctatt
     2401 gagcctatcc gaaaactttt ttaactcatc gtaacgtgac atatttgcct tctgtaaaaa
     2461 acgaagttac aaataatcaa ccgaaatgga ctgtcatttc aatttgaaat ttctattgct
     2521 gctttttcat tagctttaaa tacttaattt ctcaataaat acttagcttt taaatccccg
     2581 gaatattcct ctactttgca tttttgacct ttatacttaa ttgtatgata ccaggcaccc
     2641 at
//
LOCUS       DAOOMC010000212         2591 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdus sp. isolate AMR_MDS_2685
            NODE_49031_length_2591_cov_6.406940, whole genome shotgun sequence.
ACCESSION   DAOOMC010000212 DAOOMC010000000
VERSION     DAOOMC010000212.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864760
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdus sp. (wastewater metagenome)
  ORGANISM  Syntrophorhabdus sp.
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae; Syntrophorhabdus.
REFERENCE   1  (bases 1 to 2591)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2591)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,729
            Genes (coding)                    :: 2,708
            CDSs (with protein)               :: 2,708
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 7 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2591
                     /organism="Syntrophorhabdus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49031_length_2591_cov_6.406940"
                     /isolate="AMR_MDS_2685"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2699756"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(60..812)
                     /locus_tag="PK125_13495"
     CDS             complement(60..812)
                     /locus_tag="PK125_13495"
                     /inference="COORDINATES: protein motif:HMM:NF012369.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HPB39158.1"
                     /translation="MEHKRPVIASVSSGKGGVGKTFVTVNIGAYLAHRGKKVLIVDCD
                     LGLANIDVMIGVKPSLTLKEAIFEDKIIDDIAIHTKHGFDFVPASSGAIEMAQLMYED
                     IEKIKEGLTRLAQHYDTIILDTGAGISESVLQFNLFANKNIILLNRELTSLTDAYATI
                     KVIYQAFGRNSFNIIVNSTKNEEEAKRIFNHIDSICKRFLGFPLQYLGHIVYDDVVPR
                     SIMKQETLLTHQDQSIPAINCASIAENIVSWQ"
     gene            complement(802..1647)
                     /locus_tag="PK125_13500"
     CDS             complement(802..1647)
                     /locus_tag="PK125_13500"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HPB39159.1"
                     /translation="MNRSPLPEDRHTIAKILSKKNLERPLLTKISGSKPKAMLILLDY
                     LSRHERQIVENITHPIIDLEDDLLKKIVELEEENARLRSQALIDGLTGLYNNRFFRLQ
                     LETEMARTRRTGLSCCLLMIDLDNFKMINDTRGHLEGDRFLAQTAQTFSQCVRAVDIV
                     CRYGGDEFVAILPSTRQFVAIRIANRFKNAVREIPGATGYNVSASIGIAEFTVSSPWD
                     ENKFIQSADFAMYEAKSKGGNQVCTTGYHRKIDGGLEPVSIQEKDAISSIRDSEQGQG
                     ERDGA"
     gene            complement(1697..2431)
                     /locus_tag="PK125_13505"
     CDS             complement(1697..2431)
                     /locus_tag="PK125_13505"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025217.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HPB39160.1"
                     /translation="MNKFYQSIARHYDHIFPLSDGLKKFLYTFGITNTDHILDIGCAT
                     GEVVFYLAPYAQSVTGIDLDPDLIEIARSKKNERGRKNVAFFVRDMLDIDNVFIPDQF
                     RIVLCLGNTLVHLLSPDTINNFLGKVSNILSGNGMFIFQILNYEKILAQRTRELPLID
                     NDKIIFRRSYDHDTQKPLLVFDTSLTVKASSETINNSITLYPLRQEELIQMSNRHLYR
                     SIQFYGGFDGRAFNAQHDLLIGVWQK"
BASE COUNT          699 a          535 c          562 g          795 t
ORIGIN      
        1 gaaaggccag gtttatggac tgccgggaag cgatgaacta tagaaggctc attacctttt
       61 tactgccatg agacaatatt ctcggcaatt gatgcgcagt ttatggcagg aatcgactgg
      121 tcctggtggg ttaaaagggt ctcctgtttc ataatggagc ggggaacaac atcgtcatac
      181 acgatatggc caagatattg aagaggaaaa cccagaaacc tcttacaaat ggaatcgatg
      241 tggttgaaaa ttctctttgc ttcttcctca ttctttgtac tattcactat aatattgaat
      301 gaatttctac caaaagcctg gtatataacc tttatcgttg cgtaggcatc ggttaagctt
      361 gtcagttccc tattgagtaa gattatattt ttgtttgcaa ataagttaaa ttgaagaaca
      421 ctctctgata tgcctgcccc tgtgtcaagt atgatcgtgt cataatgttg ggcaagtctg
      481 gtcagccctt ctttaatctt ctcaatatct tcatacatta actgcgccat ctcgatagcg
      541 cctgaactgg cgggaacaaa atcgaatccg tgcttggtgt ggatggcgat gtcatcaata
      601 atcttgtctt caaaaatagc ctctttgagg gtaaggctgg gttttactcc aatcataaca
      661 tcaatgtttg ccaaaccaag atcacaatca acgataagta cttttttccc ccgatgtgcc
      721 agataagcac ctatgttcac cgttacaaat gttttgccaa ctccgccttt gccgcttgaa
      781 actgaggcga taaccggacg tttatgctcc atctctttct ccttgaccct gctcactgtc
      841 acgtatgctc gatatggcat ctttttcctg aatacttacc ggttcaagtc cgccatcaat
      901 ttttctgtga taaccagtgg tacagacctg gttcccgcct ttgctttttg cttcatacat
      961 ggcaaagtca gcagactgga tgaatttgtt ctcgtcccag ggagatgata cggtaaactc
     1021 ggcaataccg atactggcag acacattgta ccctgtggct cctggtatct cccgcacggc
     1081 atttttaaag cggtttgcaa ttcgtatcgc cacaaattgc cgagtagaag gcagaattgc
     1141 tacaaattca tcaccaccat agcggcaaac tatgtcaact gccctgacgc actgagagaa
     1201 cgtttgtgct gtttgggcaa gaaatcgatc accttcaaga tggccccggg tatcattgat
     1261 catcttaaaa ttatccagat cgatcatcaa tagacaacat gacaaccctg ttctcctggt
     1321 tcttgccatt tcagtttcaa gttgcaatct aaagaaacga ttattgtaaa gaccagtgag
     1381 cccatcaata agtgcttgtg atcgtaagcg ggcattttct tcttcgagtt ccacaatttt
     1441 tttgagtagg tcatcttcaa gatcaataat gggatgagtg atgttttcga cgatttgcct
     1501 ttcatggcgg gaaaggtagt ccagtagaat aagcattgct tttggcttgg atccactaat
     1561 cttggtgagg agaggcctct ctaaattttt ctttgagagt attttggcaa tggtgtgcct
     1621 atcctctggc agaggtgaac gattcatcaa acaacattat cggcataata gaagattcct
     1681 gaaggaaagg caggggctat ttttgccaca ccccgataag aagatcgtgc tgcgcgttaa
     1741 aggctctccc atcaaatccc ccatagaatt gaattgatcg gtacaaatgt ctgttcgaca
     1801 tctgtatcag ttcctcttgt ctaagaggat aaagagtgat tgaattgttg attgtttcgg
     1861 aggatgcttt cacagtaagg gacgtatcaa agacgaggag aggcttttgc gtgtcatgat
     1921 cgtaggagcg tctgaagata attttgtcgt tgtcgatgag ggggagttcc ctggttcttt
     1981 gcgcaaggat cttttcatag ttgagtatct ggaaaatgaa cataccgttg ccactgagaa
     2041 tattagatac ttttccaagg aaattgttga ttgtatccgg tgaaagtaga tggacaaggg
     2101 tatttccaag gcacagtacg atcctgaact gatctggaat aaacacatta tcaatatcca
     2161 acatatctct aacaaaaaaa gcgacatttt tcctgccacg ttcatttttt ttagagcgtg
     2221 caatctcaat tagatcagga tccagatcaa ttccggttac agactgggca tatggcgcaa
     2281 gataaaatac aacctcacct gttgcgcaac ctatatcgag gatatggtcg gtgtttgtga
     2341 taccaaatgt gtaaaggaac ttttttaacc cgtcactcag cggaaagata tggtcgtaat
     2401 gtcgggcaat tgattgatag aacttgttca tagtgcccta ttaatattga ttgtaatgta
     2461 ttttttcaaa cgtcaaatta gcgtgaggga cgggtttttt tgtttctgca gggtacccta
     2521 tgcttattat ggattctact ttaaggtgtg cgggtattcc cagaagtttt tgtacatact
     2581 cttcagctgt c
//
LOCUS       DAOOMC010000213         2506 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdus sp. isolate AMR_MDS_2685
            NODE_51427_length_2506_cov_3.979600, whole genome shotgun sequence.
ACCESSION   DAOOMC010000213 DAOOMC010000000
VERSION     DAOOMC010000213.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864760
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdus sp. (wastewater metagenome)
  ORGANISM  Syntrophorhabdus sp.
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae; Syntrophorhabdus.
REFERENCE   1  (bases 1 to 2506)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2506)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,729
            Genes (coding)                    :: 2,708
            CDSs (with protein)               :: 2,708
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 7 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2506
                     /organism="Syntrophorhabdus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51427_length_2506_cov_3.979600"
                     /isolate="AMR_MDS_2685"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2699756"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            267..467
                     /locus_tag="PK125_13510"
     CDS             267..467
                     /locus_tag="PK125_13510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB39161.1"
                     /translation="MNKARVKEENIEDRRMTEETKTFPLKLPFSLWHESKQIVAAQLP
                     ITTLHSYIIEAIREKNERAKEK"
     gene            470..1270
                     /locus_tag="PK125_13515"
     CDS             470..1270
                     /locus_tag="PK125_13515"
                     /inference="COORDINATES: protein motif:HMM:NF013793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HPB39162.1"
                     /translation="MGKILSLLNEKGGVGKTAVACQLAYFLVAQDKKVLFIDIDHQGN
                     STKSITLSKKALVSKTTSSRLLKEKVSNIEDASFVLVPTDEELRKMEKQPDKHNLFAT
                     NLKAFLDAVSPRFEYVVFDTNPNYDIRSVAALVVSDYVLSPIQLNQEALDGVGSLIGS
                     INKVRQALNNNLQLIGILPNLVEHTPFQKANFTQLVEAFSQLLIVLDGQDRTVAYIPT
                     TTAIAEAQASGAAIWEIKKTSAREAWRKIKPFFMEIYDRMERGGTKHA"
     gene            1263..2243
                     /locus_tag="PK125_13520"
     CDS             1263..2243
                     /locus_tag="PK125_13520"
                     /inference="COORDINATES: protein motif:HMM:TIGR00180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HPB39163.1"
                     /translation="MLDLSKLESVTQTSDGKPILFDISEIMEDPNQPRQEFDETSLRE
                     LADNIKARGIKSPISVKPKNADGKFIINHGARRYRAAILAGLQKIPGFIDGEHADYDQ
                     IAENIHRENLTPLEIALFIKKRLASGEKKADIARGLNRDRAFVTQHAALIDPPDFLLT
                     LYHSGKCGSAVHLYELRNLYNDHTEKVVAWCEGTEEITRKGITALKDSLNGNRKEPEA
                     QAQEAAKTEKTESRRAEKPSQKEEPDKGIAPKEGEGTKQSKESVEPGKIRKPVLLVRH
                     GKKIASIILDRKPTTEGFIFIRYEDSKGKDMFEVQTSSVKIDRLIDASEG"
BASE COUNT          822 a          514 c          596 g          574 t
ORIGIN      
        1 gttatgaggg acaccccttc gggttaagaa ggacggtaat tcgggttatg agggacatca
       61 ccctttgtag agcaaagcct gttaagggct agccgcatac tcatagggtt taaagggttc
      121 tttaaagggt ttataggttc tacaggttta ccctttctcc atgagatcat gaaaaatata
      181 tttacaaaat aattgactta ctttaataat atatcttata taacatataa aagttagtta
      241 acatagttga gtcaaggaga gactctatga ataaagcaag agtcaaagaa gagaatattg
      301 aggatcggag aatgaccgaa gaaacaaaaa ctttcccgct aaagttgcct ttttccctgt
      361 ggcacgaatc aaaacagata gtagcggcgc agctgccgat caccacgctg catagttata
      421 tcattgaggc catacgtgaa aagaacgaaa gggcaaagga gaagtaacca tggggaaaat
      481 tctttcgctg ttaaacgaaa aaggcggcgt agggaaaaca gccgttgctt gccagcttgc
      541 atattttctt gttgctcagg acaagaaggt tctgttcatt gacatagacc accagggaaa
      601 tagcacaaag tccataacgc tctcaaaaaa ggcccttgtg tcgaagacta ccagcagtag
      661 gttactgaag gagaaagtat ctaacattga agacgcatcg tttgttctgg tgcctaccga
      721 cgaagaactg agaaagatgg agaaacaacc tgataagcac aacctctttg ccacaaatct
      781 taaggccttt ctcgatgcgg tgagcccaag atttgaatat gttgttttcg acaccaaccc
      841 aaattatgat atacggtcag tagctgccct cgtcgtaagt gactacgttt tgtctccaat
      901 acaactcaat caagaggccc tggatggcgt aggttcactt ataggaagca ttaataaggt
      961 taggcaggcc cttaataata atttacaatt aattggaata ctgccaaacc tcgtggagca
     1021 cacgccattt caaaaggcaa actttacgca gctcgttgag gcattctctc aactccttat
     1081 tgtcctcgat ggtcaggaca gaacagtcgc ttatattcct acaacaacgg cgatagcaga
     1141 ggcgcaggcg agcggggccg ccatatggga aatcaagaag acaagtgcga gggaagcctg
     1201 gcggaagata aagccatttt tcatggaaat atatgacagg atggaaagag gaggtaccaa
     1261 acatgcttga cctttcaaag cttgaatccg tgacgcaaac gtcggatggg aaacccatac
     1321 tttttgacat atcggaaata atggaggacc ccaatcagcc cagacaggag tttgatgaaa
     1381 cctctttgag ggagttggcc gacaatataa aagctcgtgg aattaaatct cccatatcag
     1441 tgaagcccaa aaatgctgac gggaagttta ttataaatca cggagcacgc cgctatcgag
     1501 cggcgatcct tgcaggtctt cagaagatac ccggattcat cgatggtgaa catgccgact
     1561 atgatcagat tgctgagaac atccaccgtg agaatctgac accattggaa atagccttgt
     1621 tcatcaaaaa acgccttgcc tctggtgaaa agaaagcaga cattgccaga ggattgaaca
     1681 gggacagggc atttgtaaca caacacgctg cacttataga tccaccagat tttttgctca
     1741 ccctctatca ttcagggaaa tgcgggagcg ccgttcattt atatgaattg cgaaacctct
     1801 ataacgacca tacggaaaaa gtagttgcgt ggtgtgaagg gaccgaggaa attacaagaa
     1861 aaggaataac cgccttgaag gattccctca acggaaatcg caaagaacct gaggcccagg
     1921 cacaagaggc agcaaaaacg gagaagacag agtcacgacg ggcagagaaa ccaagccaga
     1981 aggaagagcc ggataaaggc atagctccaa aagaaggaga aggtactaaa caaagcaagg
     2041 agtcagtgga gccaggcaaa atccgaaagc cagtcctcct ggtaaggcat ggcaaaaaaa
     2101 tagcctcgat aatattggat cgcaaaccta caactgaagg ttttatcttc atccgctatg
     2161 aggacagcaa ggggaaagat atgttcgagg tacagacctc gtctgtgaag attgatcgtt
     2221 taattgacgc ttcagaaggg taggggacca atccaatttt gtttaacggt taaacagatt
     2281 caagggtgct tgtagtattg cttttattat tgcaggatag gctatcgccg gaaaatgtat
     2341 tacattttct ttttgtgtgc catgattgtg atgagcgcac ccttgaactg aggagaataa
     2401 agagagaaag gaatcaccgg gaatattgaa atcacgcgga aaccttacag tccaactcgg
     2461 cgccgcaaaa gataagtgga acgccctctg caaatcacaa aacaca
//
LOCUS       DAOOMC010000214         2456 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdus sp. isolate AMR_MDS_2685
            NODE_52935_length_2456_cov_3.262391, whole genome shotgun sequence.
ACCESSION   DAOOMC010000214 DAOOMC010000000
VERSION     DAOOMC010000214.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864760
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdus sp. (wastewater metagenome)
  ORGANISM  Syntrophorhabdus sp.
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae; Syntrophorhabdus.
REFERENCE   1  (bases 1 to 2456)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2456)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,729
            Genes (coding)                    :: 2,708
            CDSs (with protein)               :: 2,708
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 7 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2456
                     /organism="Syntrophorhabdus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52935_length_2456_cov_3.262391"
                     /isolate="AMR_MDS_2685"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2699756"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(69..731)
                     /gene="plsY"
                     /locus_tag="PK125_13525"
     CDS             complement(69..731)
                     /gene="plsY"
                     /locus_tag="PK125_13525"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HPB39164.1"
                     /translation="MTNVGLKLELFGLTIRVIFQYPYLMPFYILYVLIAYLTGSIPVG
                     IMLAKLRGADPRKIGSGNIGATNVMRAAGKAIGILTLAGDILKGFIPTTIALYCGQPV
                     AIVALIGFAAFIGHLFPIFLKFKGGKGVATAVGVYLTISPFAILINVVFFIFVLFKWR
                     YVSLGSLIGTALMPLTLIVLGKSNEYIYLSLVIGLLIFIKHKKNISRLMDGKENKIDK
                     LL"
     gene            856..2181
                     /locus_tag="PK125_13530"
     CDS             856..2181
                     /locus_tag="PK125_13530"
                     /inference="COORDINATES: protein motif:HMM:NF025080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1380 family transposase"
                     /protein_id="HPB39165.1"
                     /translation="MMDLKIEFTNKEITPWGGMVLMKELIKRTGINEFLQKCPLPKQN
                     SNRGYDPIQLINNFWVSIWSGANRYEHLEVTRHDKVIQKMFGWKRMAGHKAFQRYFKK
                     FGQSENDKIFISLFQWFFNQIHFDNYTLDVDSTVVTRYGEQQGAKKGYNPKKPGRVSH
                     HPLVAFIPELRMAANFWLRSGDTHTTNNIYGFIENTFDKLKGKKIGLVRADSGFYDKK
                     ILEYLEDHTINYIIAARFYRPLKLAMAHHKAYIRLDDGIEIAETMYKSPQWNIERRFI
                     IVRQEKNIRQKATGKQLRLFGEDELYKNYRYSLFVTNLTLPAKAVWELYRQRSDAENR
                     IKELKEDFGMDSFNVNDFFATEAALNFIIMAYNLMSLFRKAILGGKTYHQLKTLRHKL
                     FNIGSYITKNGNQKILKLALAMKRRSWFMGLWERSQLFSYPVVLSLEKF"
BASE COUNT          849 a          501 c          494 g          612 t
ORIGIN      
        1 aaactgaaag gcataggaaa acaaaacaaa ccagagaaac aagagaaacc agagaaaacc
       61 aacaaaccct ataaaagctt gtcaatctta ttctcttttc catccattag tctgcttata
      121 ttttttttgt gttttatgaa tatgagaagc ccgatgacca aagaaagata tatgtattcg
      181 tttgatttgc cgagtacaat gagtgtcaga ggcattaggg ctgttccaat gagcgaaccg
      241 agcgacacat aacgccattt aaaaagtaca aaaatgaaga aaacaacgtt tatcagtata
      301 gcaaaagggc taatggtaag ataaacccct actgctgtcg ccacaccctt accgcctttg
      361 aattttaaaa atataggaaa aagatgtcct atgaaagcgg caaaacctat caaggcaact
      421 atagccaccg gttgcccgca ataaagagcg atagttgtcg ggataaaacc ctttaatata
      481 tctcctgcca aagttagaat gccgatagcc tttccagcag ccctcatgac gttagttgcg
      541 ccgatattgc cgctaccaat ttttcttggg tctgcgcccc ttaactttgc aagcataata
      601 ccaactggga ttgaacccgt aagatatgca atcaaaacat acagtatgta aaatggcata
      661 aggtaaggat attgaaaaat taccctgatt gtcaacccga aaagttcgag ttttaatccc
      721 acatttgtca ttacacccca atttctaatg gccgccatta gaaaaatagc atggaatatg
      781 atatgataac atacagttag catagtatgt ttctatatta taagaacaat taaagtatta
      841 agaaaaggat accggatgat ggatctgaaa atagaattta ccaacaaaga aatcactcca
      901 tggggaggga tggtcctgat gaaagaactc ataaaaagga ctggaataaa tgaattccta
      961 caaaaatgcc cactacctaa acagaattcc aacagaggat atgaccctat acaacttatc
     1021 aacaacttct gggtaagcat ctggagtggt gctaacagat atgaacattt agaggtaaca
     1081 agacacgaca aggtaattca gaagatgttc ggctggaaga ggatggcagg acacaaggca
     1141 ttccagaggt acttcaagaa gttcggtcaa tctgagaatg acaagatatt catctccttg
     1201 tttcaatggt tctttaatca gatacacttc gacaactata ccctcgatgt agactctact
     1261 gttgtaacac gctatggtga gcaacagggt gcaaagaaag gatacaaccc aaaaaagcct
     1321 gggagggtaa gccaccaccc cctggttgcc ttcataccag aactacgcat ggcggcaaac
     1381 ttctggttga gaagcggtga tacccatacg acaaacaata tctatgggtt catagaaaat
     1441 acctttgata aactgaaagg aaaaaagata ggacttgtaa gggctgacag cgggttctac
     1501 gacaagaaga tactcgaata cctggaagat cacaccatca attatataat agcggcaaga
     1561 ttttacagac cgctcaagct tgccatggcc caccataagg catatataag attagacgat
     1621 ggaatagaga ttgcagaaac catgtataaa agcccccaat ggaacataga gaggcgcttt
     1681 atcattgtaa ggcaggagaa aaacatcaga caaaaggcaa cagggaagca gcttcgttta
     1741 tttggggaag atgagctata caagaattac cgctacagcc tttttgtgac taatcttaca
     1801 ctgcctgcca aggcagtatg ggaactttac cgacaacgat cagatgcaga aaacaggata
     1861 aaagaactca aggaagactt cgggatggac agctttaatg tcaatgactt ctttgccaca
     1921 gaggcagccc tcaacttcat aataatggca tacaacctta tgagcctctt cagaaaggct
     1981 atcctaggag gcaagaccta ccatcagtta aaaacactca ggcataaatt gtttaacata
     2041 ggatcataca taacaaagaa cggcaaccag aagatactaa aacttgccct tgccatgaaa
     2101 cgaagaagct ggttcatggg attatgggaa aggtcacaac tgtttagcta tccggttgtt
     2161 ctgtcattgg agaagttcta atgacaaatg tgggataata tttttcaacg accctgaggg
     2221 ttgccctgat cagcaggcaa cacgttttga tttccttttt caatatcaac tacgagctat
     2281 ctttattctt tttgtttgtt ttttatcaca agctcgggtt ttttcagaaa tacctctgta
     2341 tctggaggga tgggtccggt gagccatacg ttaccaccaa taacagatct ggcgccaata
     2401 acagcccttt ctccgagtat cgttgcattg gcatagataa taacatcatc ctcaat
//
LOCUS       DAOOMC010000215         2424 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdus sp. isolate AMR_MDS_2685
            NODE_53935_length_2424_cov_4.530182, whole genome shotgun sequence.
ACCESSION   DAOOMC010000215 DAOOMC010000000
VERSION     DAOOMC010000215.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864760
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdus sp. (wastewater metagenome)
  ORGANISM  Syntrophorhabdus sp.
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae; Syntrophorhabdus.
REFERENCE   1  (bases 1 to 2424)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2424)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,729
            Genes (coding)                    :: 2,708
            CDSs (with protein)               :: 2,708
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 7 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2424
                     /organism="Syntrophorhabdus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53935_length_2424_cov_4.530182"
                     /isolate="AMR_MDS_2685"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2699756"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1625
                     /locus_tag="PK125_13535"
     CDS             <1..1625
                     /locus_tag="PK125_13535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB39166.1"
                     /translation="AEDFIGHDWNTEQKWLAEYTFDASYAMHNQIIEKASIKAYGMYL
                     FFYYLTRLRSGGDAIMRQIWENCAAKKSKTFESVRAALSGDLREVFKEYALFTLDVEQ
                     YKGKFPDSIGGYGGENPLNVNEYHQFNDNSKINADGTIEVDKPFFLTGMSIVYLKVEN
                     KATGITAPAIKFNLKEFLKNEAIAIQAVIKYRNGRTDKEDWTGYEERVFCLANESQNF
                     ETLYLVASWAEETSEMKAFFLEIEPALKEECAGAHLRISLADFGQRHENTTSHLYVSA
                     ATKQTSSWDRKVVFDLDLTLIPPISPVSMPVPFGDIKKMLPKETPEEVVEFLKYGTHY
                     DAGTHCPFAAYRIKNCRVTTVTGEKKTHEDHICHDNLGRLCEQYTSIYSETWSFSGLD
                     KEWIHMFKKAEPLLGAINVHLDPKTKQIKWVYLDIPIYANCRIDWNYKKEGTRKENSG
                     YREIKESRSGIKEEYITPQAVSANSRELPMNPDWKAKQSTPTTASGGGRKEIPLDRET
                     YNNETKTRVSTTGKHVKTIDWNLNLDVPPDIK"
     gene            1887..>2424
                     /locus_tag="PK125_13540"
     CDS             1887..>2424
                     /locus_tag="PK125_13540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB39167.1"
                     /translation="MKKILFIWALPPLILGILVMSSVTTYAGYQLEEKLDVNIMMNIK
                     WETLPDAGGNTKEQGSCFIQASGEVIKFVKEKKRRSRRLSPRVSDEINYFPQGKMNVT
                     YKFNNKHYTSEGLCAEEEAFGSKEVKGKDVQVGEPGFSLVALLGMAGKGTALQLCGDI
                     NKIQEKIFTLREGPPDDNY"
BASE COUNT          785 a          448 c          572 g          619 t
ORIGIN      
        1 gggccgaaga cttcattggt catgattgga atacagagca gaaatggctg gcagagtata
       61 cttttgacgc ttcatatgcg atgcacaacc agatcatcga gaaggccagt attaaagcat
      121 atggtatgta tctttttttc tattacttga cacgcctacg ttctggcggc gatgccatta
      181 tgcgacagat ctgggagaat tgtgcggcca aaaagtctaa aacatttgaa tcagtgcggg
      241 cggccctttc gggtgatctg cgggaggttt ttaaggaata tgcacttttc actctggatg
      301 tggagcagta caagggcaaa tttcctgact caataggggg ctacggcgga gaaaaccctc
      361 tcaatgtcaa tgaataccac caatttaacg ataattccaa gatcaatgcc gatggtacaa
      421 tcgaagttga caaacctttt ttcctgactg gaatgagtat tgtttatcta aaggtggaga
      481 acaaagccac aggaataaca gctccggcca tcaagtttaa ccttaaagag tttctcaaga
      541 atgaagcgat cgctattcag gcagtcatca agtacaggaa tggcaggact gataaggaag
      601 attggaccgg ttatgaagaa cgggtcttct gccttgccaa tgagagtcaa aactttgaaa
      661 ccctgtatct ggttgcaagc tgggcagaag aaacttcaga gatgaaagct tttttcttgg
      721 agatcgagcc agctttgaaa gaggaatgtg cgggggcaca cttgcgtatt agtttggcag
      781 actttggcca acgccatgaa aatacaactt cgcatcttta cgtaagcgca gccacaaaac
      841 aaacatcttc ctgggacagg aaagtggttt ttgaccttga tttaactttg atccctccca
      901 tcagtcccgt ctccatgcct gtaccgttcg gggatatcaa aaagatgctg cccaaggaaa
      961 ccccggagga ggtagttgag tttttgaaat atggcactca ttatgatgcg ggaacacatt
     1021 gcccttttgc agcttaccga attaagaatt gcagggttac gactgtcact ggagaaaaaa
     1081 agactcatga agatcatatt tgtcatgata atctcggtag attgtgtgag caatacacat
     1141 ccatatacag tgaaacgtgg tcgtttagtg gtttggacaa ggaatggatc catatgttta
     1201 aaaaagcaga acctctttta ggagccataa atgttcactt agatccaaag acaaagcaaa
     1261 tcaaatgggt ttatcttgat atacctattt atgcaaactg ccgtatcgat tggaactaca
     1321 agaaagaagg tacacgaaaa gaaaatagcg gatataggga aattaaagaa tcgagaagtg
     1381 gcataaaaga agagtatatc acgccgcagg cggtttcggc aaattcgagg gagctcccta
     1441 tgaaccctga ttggaaagca aaacagtcta caccaactac agccagtggc ggaggcagaa
     1501 aggagatccc tctcgatcgt gaaacataca ataatgaaac aaaaactaga gtgtcaacca
     1561 ccggcaaaca tgtgaaaacc atagactgga atttaaacct tgacgtccct ccagatatta
     1621 aatgagagag aaaattgtgg acgggttcaa attcatatat ataaaaggca gaaaggccta
     1681 acgtgaaagt atttattgtg ggcatacaga taacgttgaa gacatcagtt acaacatgaa
     1741 gctttcccaa caaagggtgg atacggtggt aaaaaaactg gtttcaaaat ataaagttga
     1801 cggtagggtg gagttggtga aataataatg tcatgatgat cctttctatc tccttacaaa
     1861 ttaaataata ttagggtgtg atcattatga aaaaaatact ttttatctgg gcattgcccc
     1921 ccttgatcct gggcatattg gttatgtcgt cagtgacaac ctatgcaggt tatcagctgg
     1981 aggaaaaact ggacgtcaat atcatgatga acattaagtg ggaaacactc ccagatgccg
     2041 gtggcaacac caaggagcag ggttcttgtt ttattcaagc gtctggagaa gtaattaaat
     2101 ttgtaaaaga aaagaagagg cgcagtcgca ggctatctcc tcgagtcagt gatgaaatca
     2161 attatttccc acagggcaag atgaatgtaa cctataagtt caataataaa cattacacta
     2221 gcgagggcct ctgtgcggaa gaagaggctt ttggatcaaa agaagttaaa ggtaaggatg
     2281 ttcaagttgg agagcctggc ttttcattag tggcattatt aggtatggct gggaagggta
     2341 ctgccctcca actttgtggg gatattaata aaattcaaga aaaaatattt accttacgag
     2401 aggggcctcc tgacgacaat tata
//
LOCUS       DAOOMC010000216         2366 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdus sp. isolate AMR_MDS_2685
            NODE_55838_length_2366_cov_6.367373, whole genome shotgun sequence.
ACCESSION   DAOOMC010000216 DAOOMC010000000
VERSION     DAOOMC010000216.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864760
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdus sp. (wastewater metagenome)
  ORGANISM  Syntrophorhabdus sp.
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae; Syntrophorhabdus.
REFERENCE   1  (bases 1 to 2366)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2366)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,729
            Genes (coding)                    :: 2,708
            CDSs (with protein)               :: 2,708
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 7 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2366
                     /organism="Syntrophorhabdus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55838_length_2366_cov_6.367373"
                     /isolate="AMR_MDS_2685"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2699756"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(18..728)
                     /locus_tag="PK125_13545"
     CDS             complement(18..728)
                     /locus_tag="PK125_13545"
                     /inference="COORDINATES: protein motif:HMM:NF023701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GSU2403 family nucleotidyltransferase fold
                     protein"
                     /protein_id="HPB39168.1"
                     /translation="MVENVDKYLADMLRVFHKHNLFQEGVELIGSWCFKFYQKYLGVK
                     EYPLRTIDIDFLIPLPYRGTRHTNFVKDLEEVGFRCDFNTDGSMYFWNSELKIEFIVP
                     EKGRGAAGIVNIKELGLKAIPLRFVNLLLDNPIFVKEDNLEILLPNPVNFCIHKLLIA
                     SRRKSLDKNLKDLEQALYTSTIVSDNVLSMLYDSLPKTWRQKILVTLEHARTELPLLK
                     SESERLLLILQRNDKYKK"
     gene            complement(709..1014)
                     /locus_tag="PK125_13550"
     CDS             complement(709..1014)
                     /locus_tag="PK125_13550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB39169.1"
                     /translation="MQVLKGILSESKVYYLNIKEEIQKRLRSLPSGSIKERNISGRKY
                     YYLQRRKENKIIHEYIGKERPDDLLKQIQERKALLAELRKVNEALKLLQRADGRKRG"
     gene            1205..1357
                     /locus_tag="PK125_13555"
     CDS             1205..1357
                     /locus_tag="PK125_13555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB39170.1"
                     /translation="MNQRRIPADNADVVHKDMSLDSLNLHTRMIWGMLEKVVSKAAIY
                     PIIPNP"
     gene            complement(1529..1858)
                     /locus_tag="PK125_13560"
     CDS             complement(1529..1858)
                     /locus_tag="PK125_13560"
                     /inference="COORDINATES: protein motif:HMM:NF014043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF86 domain-containing protein"
                     /protein_id="HPB39171.1"
                     /translation="MRDVDERLLDMVEAIERIEKYASEGRESFEQNELIQTWVVHHLE
                     IIGEAASKLGRAFHEAHPVIPWAQIIAMRNILIHEYFGMDLGAVWNTIERDLPELKAK
                     LKAIIKK"
     gene            complement(1855..2145)
                     /locus_tag="PK125_13565"
     CDS             complement(1855..2145)
                     /locus_tag="PK125_13565"
                     /inference="COORDINATES: protein motif:HMM:NF014021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HPB39172.1"
                     /translation="MDNRTLLKTKKDMILEVARRHGARNVRIFGSIARGEDDSQSDID
                     FLVDMEPGRSILDHASLLVELQQILGPKVDVVSERGIKDRIRDRILREAIPL"
BASE COUNT          627 a          546 c          447 g          746 t
ORIGIN      
        1 cttgaacctt atttacacta ctttttatat ttatcattcc tttgtaggat aagaagcaat
       61 ctctctgatt cactctttaa caatggtaat tcagtcctcg catgttcaag agttacgagg
      121 attttctgtc tccaagtctt tggcaatgaa tcatacagca tactaaggac gttatcgctt
      181 actatcgtcg aagtgtagag tgcttgttca agatccttga gatttttgtc caaacttttt
      241 ctccgagatg cgatgaggag tttatgtata cagaaattta ccgggttcgg taaaaggatt
      301 tccaaattgt cttctttgac aaagatcggg ttatctagta acagattaac gaatcttaat
      361 gggatcgctt tgagaccaag ctctttgata ttgacaatcc ctgcggctcc acggcctttc
      421 tccggtacaa tgaattcaat ctttaactcg ctattccaga aatacatgga accatcagta
      481 ttgaaatcac aacggaagcc aacctcttct aggtctttga caaaattcgt atgcctggtt
      541 cctcgatatg gcagtggaat taaaaagtcg atatcaattg ttcgcagggg atactctttt
      601 acacccaagt acttttggta aaatttgaag caccagctac caattagctc aacgccttct
      661 tgaaacaggt tatgtttatg aaaaaccctg agcatatctg caaggtattt atccacgttt
      721 tctaccatcg gccctctgga gtaacttgag agcctcgttt accttcctga gttctgcaag
      781 cagcgccttt ctttcctgaa tctgcttgag caaatcgtcg ggccgttcct tgccgatata
      841 ctcgtgtatg attttatttt cttttcttcg ctgtaaataa taatattttc tgccagaaat
      901 gttcctttct tttatgcttc ccgaaggcaa agagcgaagc cttttctgta tctcttcctt
      961 gatattgaga tagtacactt tgctttcgga tagaatacct ttgagaacct gcatgatttc
     1021 gtcacccctt ttacactaca ttatagcata tctttcagat tgtagtgtaa ttcatctatt
     1081 gtgacctttt tcttcatgaa gcctttaact gtttttcaaa acgaagcagc atgtgaaggc
     1141 tgatgacaac gggaacacac aatttgcaaa aataaccggc agatcaccta tcataagccc
     1201 atgaatgaac cagaggagaa tccccgcaga taacgcagat gttgtccaca aggatatgtc
     1261 cctcgattcc ctcaacctgc acacccggat gatctggggg atgctggaaa aggttgtaag
     1321 caaggctgcc atatatccga tcataccgaa tccgtgatcc atattttcac catgtacctt
     1381 ttgttccatt ctttctccga ggttatgaac tcttgctgct cttaaataat acatcatcgg
     1441 actgacaact gtatgtcatg gtgttcggca tgctaaaaaa ttatttttga atttatagtt
     1501 ggtccttgct ggagaggtaa tgaagatatt atttctttat tattgctttg agtttggctt
     1561 tgagttccgg taagtcccgc tctatggtgt tccagactgc ccccaagtcc ataccaaaat
     1621 actcatggat caggatgttc cgcattgcaa taatctgtgc ccagggaatc acaggatggg
     1681 cctcgtggaa tgcacggccg agcttcgatg ctgcttcacc gatgatctca aggtggtgaa
     1741 cgacccatgt ttgaatcaac tcgttttgct caaaactttc cctgccctcg gaagcatact
     1801 tttcgatacg ttcaatggct tcaaccatat cgagcaaacg ctcgtctaca tctctcatag
     1861 aggaatcgcc tcgcgaagga ttctatcgcg aattctgtct tttatacccc gctcgcttac
     1921 cacatccacc ttaggcccaa gaatttgctg aagctcaacc aaaagggatg catgatcaag
     1981 aatgcttcgg ccaggctcca tatcaacaag aaaatcaata tcactttgtg aatcatcctc
     2041 gccacgggca atggagccaa aaatacgcac atttcttgct ccatgtcgcc tggctacttc
     2101 cagaatcatg tctttttttg tctttagcag cgttcgattg tccatattgt tagcctcttc
     2161 attacatctc ttattttcca gtaattgtca gagttttgca atgaaaatat gaacaaacag
     2221 tacttgaaag gttaggttcc acattcgcgc acatcgtctt aatcatcccc ccccatcgat
     2281 tgagtaaagg tattgtttgc aaagagtgac acaaactgtc actctttgca aataggagct
     2341 ttccagatag gctgcgctcg catgct
//
LOCUS       DAOOMC010000217         2361 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdus sp. isolate AMR_MDS_2685
            NODE_56000_length_2361_cov_4.674328, whole genome shotgun sequence.
ACCESSION   DAOOMC010000217 DAOOMC010000000
VERSION     DAOOMC010000217.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864760
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdus sp. (wastewater metagenome)
  ORGANISM  Syntrophorhabdus sp.
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae; Syntrophorhabdus.
REFERENCE   1  (bases 1 to 2361)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2361)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,729
            Genes (coding)                    :: 2,708
            CDSs (with protein)               :: 2,708
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 7 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2361
                     /organism="Syntrophorhabdus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56000_length_2361_cov_4.674328"
                     /isolate="AMR_MDS_2685"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2699756"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..431
                     /locus_tag="PK125_13570"
     CDS             <1..431
                     /locus_tag="PK125_13570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB39173.1"
                     /translation="KYGNNHTIERGDDDQNRRGACSFSLALPTGKPLYGLFQKEEDLK
                     GCFLVTRAVFEAYGLPSCFYLDGASQFTTIRHGGIHISQNDTEPTQFEWAMGEPGIQL
                     IFAGSPQTRGRGERINQTFQDQLVSELRLHTITTAEAATQ"
     gene            549..803
                     /locus_tag="PK125_13575"
     CDS             549..803
                     /locus_tag="PK125_13575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB39174.1"
                     /translation="MTKGQIIQLLPIKTQRHFVKASVTVNHWTDDSWHVLHETYGEIP
                     ENRSLLRARLDPFIGSGPDEGRACLWGWQNHSACGIFMLR"
     gene            complement(870..1577)
                     /locus_tag="PK125_13580"
     CDS             complement(870..1577)
                     /locus_tag="PK125_13580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB39175.1"
                     /translation="MGTEHHGGIKLLIKGLITALILVLAIWRPEILVGTFTYQILFLK
                     IYHVIWAMIMWLLIKRMVPRVNSKISMGKIFEKHYSAASAGDSARHEKFQAKLADYTH
                     RMNVGAVKTAIYWLLAVLFVGILFHTGVLNRMWVILTVVFFIFMDQFCVSVWCVFKWI
                     IGNKCCNTCRINNWGYLMAFSTLVFVPSFWTYSVLAMSVIVVIQWEYLYARHPERFYE
                     LYNTNLQCRNCDGICKA"
     gene            complement(1764..>2361)
                     /locus_tag="PK125_13585"
     CDS             complement(1764..>2361)
                     /locus_tag="PK125_13585"
                     /inference="COORDINATES: protein motif:HMM:NF019284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="toxin-antitoxin system YwqK family antitoxin"
                     /protein_id="HPB39176.1"
                     /translation="VTPGIEDKTEEIRYYRGDEVIAVEVYDKKGVLIHKSGAIPDGVV
                     REYYRGGMVKSELAFKEGRAHGKSTRYYPTGEVEEECSFKYGRLHGNSRIYRRDGTIW
                     VEAEYDHGKLQGIYKSYHDNGNIETQAEYLNGKLHGHYALFDRQGIPMKEATYVYGKR
                     HGACLIYDEMGQVIRIERYEGGKLIGTGNLGEKSKATW"
BASE COUNT          650 a          593 c          483 g          635 t
ORIGIN      
        1 gaaaatacgg aaacaaccac accatcgaaa gaggagacga cgatcagaac aggagaggcg
       61 catgctcttt ctcgttggct ctccccaccg gcaaacctct ctatggcctg ttccagaaag
      121 aggaagatct gaagggatgt ttcctggtaa ccagggcagt ctttgaagca tatggtctcc
      181 cttcctgttt ttaccttgat ggggcatccc agtttaccac catccgtcat ggaggtatcc
      241 atatatccca gaatgataca gaacccaccc agtttgaatg ggccatggga gaacccggca
      301 tacagctcat ctttgcaggc tctccccaaa ccagaggaag aggagagagg atcaatcaga
      361 cctttcagga tcagctggta tctgagctca ggctccatac aatcactact gcagaggctg
      421 caacacaata ataccgaagt tcagcaaacg attcggtgta aaaccagagg atacaacccc
      481 tgcatggagg cacacgagtg caaagttacc agatattctc tgtacacggt atcagaggaa
      541 ggtgaataat gacaaagggg cagatcatcc agcttctccc tataaagaca cagaggcatt
      601 ttgtgaaggc atcggttact gtcaaccact ggactgacga ctcatggcat gtactccatg
      661 aaacctatgg tgagatacct gagaaccgat ccctgttgcg agcccggctg gaccccttca
      721 tcggctcagg gccggacgaa gggagagcat gtctttgggg gtggcagaat catagtgcat
      781 gtggcatttt tatgttgcgt taatactgtt tccggtaaat aaaagagttg cttctttcgt
      841 ttcctatagc cctttgttca tagccctctt tacgccttac aaatcccgtc gcagttcctg
      901 cactgaagat tggtgttgta aagttcatag aatctttcgg gatgcctggc atagagatat
      961 tcccactgga tcaccacaat aaccgacatt gccagaacag aataagtcca gaaagagggg
     1021 acaaaaacaa gcgtggagaa ggccatgagg taaccccagt tatttattct gcaggtatta
     1081 cagcacttat tccctattat ccacttaaaa acgcaccaga ctgatacaca aaactggtcc
     1141 atgaagataa aaaaaactac agtcaggatg acccacatac gattaagcac cccggtatgg
     1201 aaaaggatac caacaaaaag tacggcaagt aaccaataga tagctgtttt cacagcaccg
     1261 acattcatac ggtgagtata atcagcaagt tttgcctgga atttttcatg tctggccgag
     1321 tcaccggcag atgcagcaga ataatgtttc tcaaaaatct tccccattga aatcttggaa
     1381 ttcacccgtg gaaccatacg cttgatgaga agccacataa tcatagccca tatgacgtgg
     1441 taaatcttta aaaaaaggat ttgataggta aaggttccga caagaatttc cggccgccag
     1501 atagccaaaa caaggatcaa cgctgtgatt aagcccttaa tgagtaactt tattcctccg
     1561 tgatgttcgg tacccatttt cagttatcct ttccacaaaa cagaaccggt aatgttcctg
     1621 acgcttaaca ttatgttaca gcatttattg acaatactgc tccaagatgg atagggtctg
     1681 acatgacgcc ttatcactcc ctgtgataaa aatccctttc tcttcattcc atcacttcat
     1741 acatgtttta aaggactttg gctttaccat gtggccttgc tcttttctcc caaatttcca
     1801 gtaccaatga gtttcccacc ctcatatctc tcgattctga taacctgccc catctcatcg
     1861 taaatcaggc acgcgccgtg cctcttaccg tagacatagg tggcttcttt cataggaatt
     1921 ccctgcctat cgaataaagc atagtggccg tgaagttttc catttaggta ttctgcctgg
     1981 gtttcgatgt ttccgttgtc atgataactt ttatatattc cttgaagctt gccgtggtca
     2041 tattctgcct ctacccatat cgtgccatcc cgtcggtaaa tcctgctatt accgtgcagc
     2101 ctgccatatt taaaagaaca ttcttcttcg acctctcctg tggggtagta tctggtgctt
     2161 tttccgtgag ccctcccttc tttgaaggct aactcactct tcaccattcc cccgcggtaa
     2221 tattctctga ccacaccatc tggtattgca cctgatttat gaattaggac acctttttta
     2281 tcatagacct caacggcgat tacctcgtct ccgcgataat aacgtatctc ttctgtctta
     2341 tcctcaatcc ccggggtgac a
//
LOCUS       DAOOMC010000218         2350 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdus sp. isolate AMR_MDS_2685
            NODE_56350_length_2350_cov_3.828758, whole genome shotgun sequence.
ACCESSION   DAOOMC010000218 DAOOMC010000000
VERSION     DAOOMC010000218.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864760
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdus sp. (wastewater metagenome)
  ORGANISM  Syntrophorhabdus sp.
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae; Syntrophorhabdus.
REFERENCE   1  (bases 1 to 2350)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2350)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,729
            Genes (coding)                    :: 2,708
            CDSs (with protein)               :: 2,708
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 7 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2350
                     /organism="Syntrophorhabdus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56350_length_2350_cov_3.828758"
                     /isolate="AMR_MDS_2685"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2699756"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            464..1681
                     /locus_tag="PK125_13590"
     CDS             464..1681
                     /locus_tag="PK125_13590"
                     /inference="COORDINATES: protein motif:HMM:NF033550.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HPB39177.1"
                     /translation="MNASLLYQAYGVKNYSYGSTEYKDNAIFLNLKTILPQKHVCPHC
                     GSRHVIRNGTVLRHIHNLPFGSKACYLSLRIQRYWCKECEKAWQSDIPFTHGEVSYTY
                     RFSRYVLDLLRMGNTILDVARHLHVGWNMVKGIHKKYLKDKYSRPDIKGVSRIGIDEF
                     ATRKGHVYKTIVVDLDTGRIIHVGDGKGKEALAGFWKRVRRNKVKIEIVTSDLSAAFI
                     ASVMENVPDAIHVYDKFHVSRLVTDAVDKVRRSVYAQETDLEKRKIIKGSRWLLLTKD
                     KDVFDDEKKRRLDNILTTNTPLFHAYYLKEDLDQIWSRESKEEGGKQLAYWCERARET
                     KLQPFVKVANTLMASRTGILAWYDAPLTNAILEGINNKIKVMKRRAYGYRDHEYFNLL
                     LLGLHDKTNAIRG"
     gene            1653..>2350
                     /locus_tag="PK125_13595"
     CDS             1653..>2350
                     /locus_tag="PK125_13595"
                     /inference="COORDINATES: protein motif:HMM:NF033520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HPB39178.1"
                     /translation="MTKPTQFGDEPLIKGTLKSYFPNAVSYNRFVELESRVFFPLMFF
                     LNLRAFGRCTGITFVDSTMIPVCHNLRRYANKVFKGMATDGKGTMGWCHGFKLHLACN
                     DRGEIVAFLLTGANVSDKDPEVFKVPARRLYGKLFADKGYISQKLFDFLFEDGIQLVT
                     GLRVNMKNKLMPFHDRMMLRKRYIIETINDMLKNKAQIVHTRHRSLSNFIMNLISALG
                     AYCFFDNKPQALVG"
BASE COUNT          690 a          511 c          531 g          618 t
ORIGIN      
        1 gacgggttct gttattgtta gtttcggact gtcttatccg agaatcttct tgttttcggg
       61 tgcgaattta atgctttttc ttgaatcaag caagatttct atcgattttt agcaaaagga
      121 ttgcgaatat gccaactttt ttataacttt gccttcagca atcatggcga ctgttgttta
      181 caattctttg taaatcgacg caataatacc ttatactgaa acaggtagat acatttcaaa
      241 acaattataa ttcatcgaac tcaccttatt ataacgttga ttgacaatct atcatgttac
      301 ttaagatatt ccgtgaatca ttactgccca ctaaatagtt attaaaatac gtatgactct
      361 atgaaaaaaa aggttcatcc ccgaattgcg ttgtttttgt agtttcctat catatgaaaa
      421 gctgacaaat cattatcttt gtggttcccc aaccatatca gatatgaatg ccagcttatt
      481 atatcaggcc tacggagtca agaactattc gtatggcagt acagagtaca aagataatgc
      541 aattttcctc aatctcaaaa caatcctgcc ccaaaagcat gtatgccctc attgtggcag
      601 ccgccatgtt atacgaaatg gtacggttct tcgtcacatc cacaatcttc ctttcggcag
      661 caaagcctgc tacctgtccc ttcggataca acgctattgg tgcaaggagt gtgagaaggc
      721 atggcagtcc gacattcctt ttactcacgg agaggtaagc tacacctaca ggttctcccg
      781 ctatgtgctt gacctgctgc gaatgggcaa caccatcctg gacgttgccc gacacctgca
      841 tgtgggctgg aacatggtaa agggcatcca caagaagtat ctgaaagaca aatactcccg
      901 tcctgacata aagggtgtaa gccgcatagg gattgatgag ttcgccacca gaaaggggca
      961 tgtctacaag actattgtcg ttgaccttga cacaggccgc atcatccatg tgggggacgg
     1021 gaagggaaag gaagcacttg ctggattctg gaaacgggtc agacgcaaca aggtgaagat
     1081 agagattgtc acctcagacc tctcggcggc attcattgcc tctgtgatgg agaatgtccc
     1141 cgatgcaatc cacgtatacg acaagttcca tgtctccagg ttggttacgg atgctgttga
     1201 caaggtgcgc aggagtgtct acgcccaaga gacagacctt gagaaaagga agataataaa
     1261 aggctcaaga tggctgttgc tcactaagga caaggatgtg ttcgacgacg agaagaagag
     1321 aagacttgac aatatcctca ctaccaatac accgctgttc catgcatact atctcaagga
     1381 agaccttgac cagatatgga gccgggaaag taaagaggaa ggagggaagc agctggctta
     1441 ttggtgcgaa agggccagag aaacaaagct acagccgttt gtaaaggtcg ccaacacact
     1501 gatggcaagc aggaccggaa tacttgcatg gtatgatgcg ccactgacga atgccatcct
     1561 tgaaggcatt aacaacaaaa tcaaggtcat gaagcgcagg gcatatggct atagagacca
     1621 cgaatacttc aatctactac tgctcggatt gcatgacaaa accaacgcaa ttcggggatg
     1681 aaccccttat taaagggaca ttgaaatcat atttcccaaa tgccgtttca tacaaccgtt
     1741 ttgttgaact tgaaagcagg gtgttcttcc ctctgatgtt tttcctcaat ctgcgggcct
     1801 ttggcagatg tacagggata accttcgtgg attcaaccat gatacctgta tgtcataacc
     1861 tcagacgtta tgccaataag gtgttcaagg gcatggcgac agacggtaag ggcactatgg
     1921 gatggtgcca tggcttcaaa ctacatcttg cctgcaatga cagaggcgag attgttgcat
     1981 tcctgctcac tggagcgaac gtaagtgaca aggacccgga agtgttcaag gtgccggcta
     2041 ggcgactgta tggcaaactg tttgctgaca agggatatat ctcacagaaa ctctttgatt
     2101 ttctctttga ggatggaatc cagctggtta caggtctcag ggtgaacatg aaaaacaaac
     2161 tcatgccttt ccatgacaga atgatgctgc gcaagagata catcatcgag actatcaatg
     2221 acatgctaaa aaacaaggct caaattgtgc atacacgtca taggtctctt tcaaatttca
     2281 tcatgaatct aatatctgct ttaggtgcat attgtttctt tgacaataag cctcaagcac
     2341 ttgtaggata
//
LOCUS       DAOOMC010000219         2342 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdus sp. isolate AMR_MDS_2685
            NODE_56631_length_2342_cov_6.062090, whole genome shotgun sequence.
ACCESSION   DAOOMC010000219 DAOOMC010000000
VERSION     DAOOMC010000219.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864760
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdus sp. (wastewater metagenome)
  ORGANISM  Syntrophorhabdus sp.
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae; Syntrophorhabdus.
REFERENCE   1  (bases 1 to 2342)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2342)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,729
            Genes (coding)                    :: 2,708
            CDSs (with protein)               :: 2,708
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 7 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2342
                     /organism="Syntrophorhabdus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56631_length_2342_cov_6.062090"
                     /isolate="AMR_MDS_2685"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2699756"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1321
                     /locus_tag="PK125_13600"
     CDS             <1..1321
                     /locus_tag="PK125_13600"
                     /inference="COORDINATES: protein motif:HMM:NF024118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HPB39179.1"
                     /translation="HFLMMQLKIHRGTHEIGGSCVEITSHDSRIVIDIGMPLVKEGGG
                     KFDLKEHKDLTGPELVQTGVLPDIKGLYEWDTSNKPIDGLLISHAHMDHYGFLPYINQ
                     NVHCYLGEGTKRLIDITSLFLAFKTAINRHTFIKSGEPLWVGAFKVTPYLMDHASFDA
                     YAFLVESDSKKVLYTGDFREHGRKAKAFQWFLLNAPKDIDVLLLEGTMVGNRKEASKT
                     EGDIEQEFVHAIKDTEGIVLALLSGQNIDRIVSLYRATVRTGRTFVVDVYTANVLADL
                     HDLAKIPYLSKEFDRIRVLFPCRLSKKIATMGRKDLLYRFKTSKITKNEIMEKPDTMV
                     MLVRSSMISDLASMKNIDGATVIYSMWQGYLNESSMKPFLDFTQKKNMQLVSVHTSGH
                     AYIKTLQKVVKTLRPKTIIPIHTFSPERYSTLFSNVVQTLDGQEISV"
     gene            1318..1824
                     /locus_tag="PK125_13605"
     CDS             1318..1824
                     /locus_tag="PK125_13605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB39180.1"
                     /translation="MTGARQERGFEPFAIDAMGEALLEQLTNQGWDVRIEDDWAGDFK
                     DYWCTSPRGPMGAFTFLDLLLFTLDNDPENVEIRFMFYADPGIVGPNKRPFGKKVREN
                     NFRVLRDFCVSLLNGANIGSNEQETLITPSSRRKVKFGDAYVAPSYEVQLVYWREPER
                     RLNARKST"
     gene            1805..2068
                     /locus_tag="PK125_13610"
     CDS             1805..2068
                     /locus_tag="PK125_13610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB39181.1"
                     /translation="MPGRVPDDPESTTEEVRKRCGLPENACCGNCAEREDCMGLGWDD
                     DCDDDPRDDVCGSWVLSPPYEGKLYEVIDNKVVTTYGVEFEDD"
BASE COUNT          675 a          508 c          580 g          579 t
ORIGIN      
        1 tcatttttta atgatgcagc tcaaaatcca ccgtggcact cacgaaattg gcggttcctg
       61 tgttgagata acaagtcatg acagcaggat tgtcatagat attggcatgc ccctcgttaa
      121 agaaggcggt ggcaaattcg atctcaaaga acataaggac ctcacaggcc cggaacttgt
      181 tcagacagga gttttgcccg acatcaaagg cctctatgaa tgggatacgt cgaacaaacc
      241 aatcgacgga ttgctcatct ctcatgccca catggatcat tatggcttcc tgccttacat
      301 aaaccaaaac gtccattgct accttggtga aggcaccaag agactgatcg atataaccag
      361 cctgtttctg gcatttaaga ccgcaatcaa caggcatacc ttcataaaaa gtggagaacc
      421 cctatgggta ggagcattca aggtaactcc ttacctcatg gatcacgctt cttttgatgc
      481 ttatgctttt ctcgttgaat ctgacagcaa aaaggtactc tacacggggg acttcagaga
      541 acacggcagg aaggcaaagg catttcagtg gtttttgctg aatgccccga aagatattga
      601 cgtcctcctg ctggagggaa ccatggttgg caatcggaaa gaagcatcca agacagaagg
      661 tgatatcgaa caagaatttg ttcatgcaat caaggatacc gagggaatag tcctggctct
      721 tctctcaggt cagaacatcg acaggatagt gtcgctctac agggctacgg tacggacagg
      781 gcgaaccttc gtggttgatg tatacacggc caatgtcctt gcagacttac atgacttggc
      841 aaagattccg tatctatcaa aagaattcga caggatcagg gtgttgtttc cttgccggct
      901 ctcaaaaaaa atcgcaacaa tgggaaggaa agatctcctc tacagattca agacctccaa
      961 gatcacaaag aatgagatca tggagaagcc ggatacaatg gtcatgcttg taaggtcatc
     1021 catgatatct gatcttgcat ctatgaaaaa tattgatggc gctaccgtca tatattccat
     1081 gtggcaaggt tacctcaatg agtcttccat gaaacctttt ttggatttta cgcaaaagaa
     1141 aaatatgcag ctcgtctcag ttcatacgag cgggcatgcg tacataaaaa ctttgcaaaa
     1201 ggtggtcaaa acattacggc ccaaaaccat aattcccatc catacttttt ctcctgagcg
     1261 gtatagcacc ttgttttcga atgtcgtcca aactctggac ggtcaggaga tttcggtatg
     1321 acaggggcca gacaagagag gggatttgag ccctttgcca ttgatgccat gggcgaggcg
     1381 ctgttagaac agctgacgaa tcagggatgg gatgtccgga tcgaagatga ttgggcaggc
     1441 gatttcaaag attactggtg tacctcccct cgcggaccca tgggcgcctt cacattcctt
     1501 gatctccttc tcttcacttt agacaatgac ccggagaatg tggagatacg gtttatgttc
     1561 tatgcagacc cgggcatagt cggccccaac aagcgacctt ttggcaaaaa ggtgagggaa
     1621 aacaacttcc gggtcttgag agatttttgc gtcagcctct tgaatggtgc aaatatcggg
     1681 agtaatgagc aagaaacctt gataacccct tcatctcgtc gtaaagtgaa atttggtgat
     1741 gcttatgtag ctccctccta cgaggtacaa ctggtttact ggagggaacc ggaaaggagg
     1801 ttgaatgccc ggaagagtac ctgatgatcc tgaaagcacc acagaagagg tgcggaaaag
     1861 atgcggcttg cccgaaaatg cttgctgcgg gaattgtgct gaaagagaag attgtatggg
     1921 cttagggtgg gatgatgact gcgatgatga ccccagggac gatgtctgcg ggagctgggt
     1981 tttgagtcca ccatatgagg gtaaacttta cgaagtcata gacaacaaag tggtaacaac
     2041 gtatggggta gagtttgaag acgattagga atagcctgaa agagcctctc tatcccaatc
     2101 gctgacccct ctttgatggt gatccaagtg acgctgcaat cccgcagtac aaaaaaagtt
     2161 taaaatttct ttgttagtat ttcatcactg acatacaggt ggcagtatcg ttgtgtataa
     2221 tccataatag aggcgattca agactgcaga ttcaagattc aagatcgggg aggttatcat
     2281 gacacagtac accttttaca tcaacggaga gtacaggaca gtcgaggcag ataccgagga
     2341 gg
//
LOCUS       DAOOMC010000220         2324 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdus sp. isolate AMR_MDS_2685
            NODE_57248_length_2324_cov_6.622741, whole genome shotgun sequence.
ACCESSION   DAOOMC010000220 DAOOMC010000000
VERSION     DAOOMC010000220.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864760
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdus sp. (wastewater metagenome)
  ORGANISM  Syntrophorhabdus sp.
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae; Syntrophorhabdus.
REFERENCE   1  (bases 1 to 2324)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2324)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,729
            Genes (coding)                    :: 2,708
            CDSs (with protein)               :: 2,708
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 7 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2324
                     /organism="Syntrophorhabdus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57248_length_2324_cov_6.622741"
                     /isolate="AMR_MDS_2685"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2699756"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(38..949)
                     /locus_tag="PK125_13615"
     CDS             complement(38..949)
                     /locus_tag="PK125_13615"
                     /inference="COORDINATES: protein motif:HMM:NF016362.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HPB39182.1"
                     /translation="MAVPDFQSFFKPLLDIAADGKEHTIQEAREIIAKKMNLTQKDLE
                     ELLPSGTQMKFDNRVAWAKSYFTQAKVLESPRRSYFKITDRGRELHKKGHERIDIKIL
                     EQYQEFVAFHTAGRAKPPKGTGEGQDTGDETPEEVLQKAYQSIRNDLAAELLEKIKQN
                     SPKFFERLVTDFMVALGYGGSLADAGRSIGQSGDEGIDGIIKEDRLGLDLIYLQAKRW
                     NGAVGRPEVQKFVGALQGKRARKGVYITTGKFTDDAVRYADSIDPKVILVDGITLANM
                     MIDHGLGTTTTATYEIRRMDSDYFTEE"
     gene            complement(1020..2231)
                     /locus_tag="PK125_13620"
     CDS             complement(1020..2231)
                     /locus_tag="PK125_13620"
                     /inference="COORDINATES: protein motif:HMM:NF019843.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HPB39183.1"
                     /translation="MAYRRSIEILPEAATYFNLALCYDDMGAKDLTQEAMNKFFELVP
                     SGEEQEEAERMLHQNGKDYLIRLDKKEALIGYRKAAEQGDSLSQYNLGKMYYYGHGVP
                     QNYNEAVKWFSKAAEQGNAKAQYVLGLLYDNGEGIPQNDKDAIRWYTKAAEQGLAEAQ
                     HNLALMYEYGRGIPQNHKEAVKWYSKAAEQGISFAQYNLGLAYDTGQDVSLDYKEAAK
                     WYLKAAKQGHAMAQNNLGVMYYKIHNHKEAVKWYSMAAEQGIARAQGNLAGMYYNGQG
                     VPQNYNEAVKWYSMAAEQGEADAQHDLALMYENGNGVPQNYKEAVRWYSKAAKQGHAI
                     AQSKLGLFFYKGQGISKDPVQAYKWFELAVTHGDKDAKKHRDTLKKKMSLDQLAEAQR
                     LIREFKTQAEE"
BASE COUNT          523 a          592 c          480 g          729 t
ORIGIN      
        1 tgtcgaacga gaatccataa cttgttattt ctgtcgttta ttcctccgta aaatagtcag
       61 aatccatcct tctgatctca tatgtagccg tagttgttgt ccctaatccg tgatcaatca
      121 tcatgttcgc caatgtgatt ccgtcgacca atatcacctt cggatcgata gagtcagcat
      181 atcttactgc gtcgtcggta aattttcctg tcgtgatgta tacgcctttc ctcgcccttt
      241 tgccctgtaa ggctcctacg aatttctgga cttcaggccg tcctaccgca ccattccatc
      301 tctttgcctg aagatagatc aggtcaaggc ccagacggtc ttccttgatg atcccgtcga
      361 ttccctcatc tcccgattgc ccaatcgatc taccggcatc ggccagggac ccgccgtaac
      421 ccaaggcaac catgaaatcg gtaacaaggc gctcaaaaaa tttaggtgaa ttctgcttta
      481 ttttctcaag aagttccgcg gcaagatcgt tccggatgct ctgataggct ttctggagga
      541 cttcttccgg ggtttcatct ccagtatctt gtccttcacc tgtcccttta ggaggtttgg
      601 ctctgccggc tgtatggaat gcaacaaact cttggtattg ttccagaatc ttgatatcga
      661 ttctttcatg gcctttttta tgtagttcac gacccctatc cgtgatcttg aagtaagagc
      721 ggcggggact ttccagaacc tttgcctgtg taaaatagct cttcgcccag gctacccggt
      781 tatcgaattt catctgagtc ccactcggaa gaagttcctc tagatccttc tgggtgagat
      841 tcattttctt ggcgatgatc tcccgggctt cctggattgt gtgttctttc ccatcagcgg
      901 caatgtccaa aaggggtttg aaaaatgatt ggaagtctgg aacggccatt ttacatcccc
      961 attctttctt ttctcaattc aaataaatcc tatttccagt taacatcata gggcgaaacc
     1021 tattcctccg cctgggtttt aaactcgcgg attaaacgct gcgcctcggc aagttgatcg
     1081 agggacatct ttttcttcaa tgtgtccctg tgcttttttg catctttgtc gccatgtgtc
     1141 acggccaatt caaaccattt atatgcctga acagggtcct ttgagatgcc ttgtccctta
     1201 taaaaaaaca aaccgagctt tgattgagct atggcatgtc cctgtttagc ggccttggag
     1261 taccatctaa ccgcttcctt gtaattttgt ggaacaccgt tgccattttc atacatcaag
     1321 gcaagatcat gttgggcatc tgcctctccc tgttcagcag ccatggagta ccatttaact
     1381 gcttcgttgt aattctgagg aacgccttgg ccattgtagt acattccggc caaattacct
     1441 tgggctcggg caatgccctg ttcagcagcc atggagtacc acttaaccgc ttctttatga
     1501 ttatgaattt tgtaatacat tacgcctaaa ttattttggg ccattgcatg gccttgcttg
     1561 gcagccttta aataccactt agccgcttcc ttgtaatcca gagatacgtc ttgaccagta
     1621 tcatatgcta agcctaaatt gtattgggca aaagagattc cttgttcggc agccttggaa
     1681 taccatttaa ctgcttcctt gtgattttgt gggatgccgc ggccatactc atacatcaaa
     1741 gcgagattgt gttgggcctc agcgagtcct tgctcagcgg ccttggtata ccacctgatc
     1801 gcatctttgt cgttttgggg tataccctca ccattgtcgt acagcaaccc taatacatat
     1861 tgtgcttttg cattaccttg ctcagcagcc tttgaaaacc acttaactgc ttcgttgtaa
     1921 ttctgaggga caccgtggcc atagtaatac atcttaccta aattgtattg ggagagagag
     1981 tcgccctgtt ctgcagcttt acggtaccca atgagggctt cttttttatc caggcggata
     2041 agatagtctt taccattctg atgcagcatg cgctctgcct cttcttgttc ctcccctgaa
     2101 ggtacaagtt cgaagaactt attcatcgct tcttgcgtaa ggtctttcgc ccccatgtcg
     2161 tcataacata atgcgaggtt aaaataagta gctgcttcgg gaagaatttc gattgaacgt
     2221 ctgtacgcca ttgatgccaa ccacctatga cctaatttcc aataccaacc cccaacctcg
     2281 catagatgtg agtgattgtg ctcccgtggt aatatttcag caag
//
LOCUS       DAOOMC010000221         2303 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdus sp. isolate AMR_MDS_2685
            NODE_58061_length_2303_cov_6.601868, whole genome shotgun sequence.
ACCESSION   DAOOMC010000221 DAOOMC010000000
VERSION     DAOOMC010000221.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864760
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdus sp. (wastewater metagenome)
  ORGANISM  Syntrophorhabdus sp.
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae; Syntrophorhabdus.
REFERENCE   1  (bases 1 to 2303)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2303)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,729
            Genes (coding)                    :: 2,708
            CDSs (with protein)               :: 2,708
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 7 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2303
                     /organism="Syntrophorhabdus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58061_length_2303_cov_6.601868"
                     /isolate="AMR_MDS_2685"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2699756"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1168)
                     /locus_tag="PK125_13625"
     CDS             complement(<1..1168)
                     /locus_tag="PK125_13625"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: protein motif:HMM:NF001126.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HPB39184.1"
                     /translation="MKLAEVIKDIPVKGVIGNTDIDITGLTKDSRSVKEGSIFFVTKK
                     SEPFITEALKKGARVIVAEKELKTEAPCFITVDDVQSALGKMASEFYGLPSKHLHVVG
                     ITGTNGKTTITFLIESLLKADGRKAGVIGTISYRYGGLELKADNTTPGATETHSLLKD
                     MYECGIRFVAMEVSSHALDQKRVEGIEFDVGIFTNLTHDHLDYHRNIDNYRSAKAQLF
                     KHYLEQSNKKKRYAILNMDDQAVREMLPGEPVQCLFYSLKNKTDAYLVNYQEDITGLS
                     LTVSLRGKTFSLQTPLVGLFNASNILASALYGYTLGMTTATMKKGMEGLQSVPGRLER
                     VKNDRGVSIFVDYAHTPDALKKVLEMLTRLKKGRLILVFGCGGDRDRAKRPVMGE"
     gene            complement(1169..1954)
                     /locus_tag="PK125_13630"
     CDS             complement(1169..1954)
                     /locus_tag="PK125_13630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB39185.1"
                     /translation="MKKKHEVSLPEVPASIFKNQLVEDKPVNIKSDRVETTQTPPAQH
                     LLTVDDRNMEPNLFIALPLVAYAIKEGFIEKDGLIFLQKEGYNTNNCKKPIDILRDKD
                     EQGLKVISRLIGKRQSLDFLKKEGIELNQDIDSERIITGIGYSIEKNKILSLYKKYLT
                     NEFQKLFPFVIQNMAIVKTGKGFEFSQAKEVYRTDQEKTESPEWTMPNVTNLSMRAAV
                     EKLALYTSKIKVYGNGNVVEQSPRAFERTRGETECIIYGRTAR"
     gene            complement(2104..>2303)
                     /locus_tag="PK125_13635"
     CDS             complement(2104..>2303)
                     /locus_tag="PK125_13635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB39186.1"
                     /translation="LYRTKGKRAPWAQETERGRGTGIEMRQPTVVISHQFSVHREKHT
                     NTDRKQWNVDCEQNNEALIVG"
BASE COUNT          562 a          544 c          434 g          763 t
ORIGIN      
        1 tttctcccat aacaggcctc tttgccctgt ccctgtcccc accgcaccca aacactaaga
       61 ttaaccttcc cttctttaga cgggtgagca tctccaatac ttttttcaac gcgtctggcg
      121 tgtgggcata atccacaaag atcgataccc ctctatcgtt ttttactctt tctaacctgc
      181 caggaacact ctgtaacccc tccatccctt ttttcattgt ggcagtagtc attccaagag
      241 tgtaaccata gagggcagac gccagaatat tcgatgcatt aaaaagaccg acaagagggg
      301 tttgaaggga aaatgttttg cctctcaggg aaacagtaag ggaaaggcct gtgatgtctt
      361 cttgataatt gacaagataa gcatcagtct tgtttttgag gctgtagaac aggcactgaa
      421 caggctcacc cgggagcatt tcacggacag cttgatcatc catgttaagt attgcataac
      481 gtttcttctt gttgctttgc tccagataat gtttaaacaa ctgtgccttg gcacttctat
      541 agttatctat attcctgtgg taatcaaggt ggtcatgggt caggtttgta aagatgccaa
      601 catcaaactc aatcccctcc acccgtttct ggtccagggc atgagaagaa acttccatag
      661 caacgaacct tattccgcat tcatacatat ccttcaaaag gctgtgggtc tctgtggcgc
      721 ctggtgtagt attgtcagcc ttgagttcca aaccgccata cctgtatgaa attgtgccga
      781 ttaccccagc cttccttccg tctgccttaa gaagagattc gataaggaac gttatcgttg
      841 tcttaccatt ggtacctgtg atacctacaa cgtggagatg ctttgatggc agtccataaa
      901 attctgatgc cattttccca agagccgatt gcacatcatc cactgtaata aaacatggag
      961 cctcggtttt cagttccttc tcagcaacaa tcactcttgc accttttttc aaggcttctg
     1021 taataaatgg ttcactcttt tttgtaacaa aaaatattga tccttctttc acagaccgag
     1081 agtctttggt cagtccggta atgtcaatat ctgtattgcc tattactcct tttaccggta
     1141 tatcttttat gacttctgcg agcttcatct aacgggctgt cctcccataa atgatacact
     1201 cggtttcacc ccttgttcgt tcaaaggctc tggggctttg ctcaacgaca ttcccattcc
     1261 cgtaaacctt tatctttgac gtgtaaagcg ccagcttttc aactgcagcc ctcatggaaa
     1321 gatttgttac attgggcatt gtccattcgg gggattccgt tttttcttga tctgtcctgt
     1381 acacttcttt tgcctgtgaa aactcaaaac ctttaccggt tttaacaatg gccatgtttt
     1441 ggatcacaaa tgggaataat ttctggaatt cattggtaag atatttcttg taaagggaga
     1501 gtatcttatt tttttctata gaatacccaa taccggtgat aatcctctcg ctgtctatgt
     1561 cctgattgag ttcgattcct tcttttttga gaaaatcaag tgactgtcgt ttaccgataa
     1621 gccttgaaat tacctttaga ccttgctcgt ctttatccct cagtatatct attggctttt
     1681 tgcaattatt tgtattgtaa ccttcttttt ggagaaaaat caaaccatct ttttctatga
     1741 aaccttcctt aatggcatat gctaccaatg gaagtgcaat aaaaagattt ggttccatat
     1801 ttctgtcgtc aaccgttaaa agatgctggg ctggtggcgt ttgtgtggtt tctaccctat
     1861 cgcttttgat attcaccggc ttatcttcga ctagttgatt tttaaaaatt gatgccggta
     1921 cttctggcaa agaaacttcg tgcttcttct tcaatgaaga ccccattacg gtactcggca
     1981 tgatgttttg tccactccac acgcgtgtaa tgttcatttt ttcaggaagc ctcaacacaa
     2041 tagacgtata cattactcca ataaaaaggc agacaaaaaa ggctcgacgg ttggcctttt
     2101 tgttcacccc actatcagtg cctcgttgtt ttgttcgcag tccacgttcc attgctttcg
     2161 gtctgtgttt gtatgtttct ctctatgaac tgagaactga tgactgataa ccacagttgg
     2221 ctgcctcatc tcaataccag tacctcttcc tctttcggtt tcttgagccc aaggcgctcg
     2281 tttgccttta gttctatata acg
//
LOCUS       DAOOMC010000222         1929 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdus sp. isolate AMR_MDS_2685
            NODE_75204_length_1929_cov_6.921025, whole genome shotgun sequence.
ACCESSION   DAOOMC010000222 DAOOMC010000000
VERSION     DAOOMC010000222.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864760
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdus sp. (wastewater metagenome)
  ORGANISM  Syntrophorhabdus sp.
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae; Syntrophorhabdus.
REFERENCE   1  (bases 1 to 1929)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1929)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,729
            Genes (coding)                    :: 2,708
            CDSs (with protein)               :: 2,708
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 7 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1929
                     /organism="Syntrophorhabdus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_75204_length_1929_cov_6.921025"
                     /isolate="AMR_MDS_2685"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2699756"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..871
                     /locus_tag="PK125_13640"
     CDS             <1..871
                     /locus_tag="PK125_13640"
                     /inference="COORDINATES: protein motif:HMM:NF019155.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SEC-C metal-binding domain-containing protein"
                     /protein_id="HPB39187.1"
                     /translation="LEDEIMRLFGSDRVSPMLAKLGMEEDMPIEHPFISKAIENAQTR
                     VEGHNFEIRKYLLEYDNVMNKQRETIYGMRHEFMRNGDVRDQILDMIDELCENIVFDC
                     APEKVYPEEWDMVSLKNRIFETFFFHIDLDSVDLKKLTREGLLDVVREKVLAVYERKD
                     KEFGDGELKAIERFIALNSVDVHWKEHLLALDHLKEGIGLRGYAQKDPLREYQRESFD
                     LFLDMMERVKVDTVQKLYAVHPAREELTREQPAMFFNIGGEQPQEKKDKKIGRNDPCP
                     CGSGKKYKKCCGR"
     gene            883..>1929
                     /gene="argJ"
                     /locus_tag="PK125_13645"
     CDS             883..>1929
                     /gene="argJ"
                     /locus_tag="PK125_13645"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.35"
                     /inference="COORDINATES: protein motif:HMM:NF003802.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004358 - glutamate N-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate
                     N-acetyltransferase/amino-acid acetyltransferase ArgJ"
                     /protein_id="HPB39188.1"
                     /translation="MIPGIRFAGIASGIKNAALDLGLVVFDEPAYITAVYTKNKVKSA
                     HILYNKRLRSNEVEALLVNSGCANACTGPEGVRDLKELSEKLTSHLKAAEGKIVFAST
                     GVIGRRLPTETMAKALPVAVKDLRKNGIDSFSRAIMTTDTYPKIARASFKGEKEYSIA
                     GVAKGAGMINPLLATMLVFIFTDYPASPEKIRRKLLQGAKESFERITVDGECSTNDSV
                     MLFTRQGREDPGSLDAFCNALFSVMKELAMMIVRDGEGATRVIHITVNGAQKKDIAEK
                     IARRIAISPLTKTAFFGCDPNWGRIIAAAGDADVPIQPAKVEIILQGEVLAKNGIEMP
                     FDEDKVKKLMEKKDL"
BASE COUNT          541 a          417 c          520 g          451 t
ORIGIN      
        1 tctcgaagat gagattatga ggctctttgg ctccgacagg gtttctccca tgcttgcaaa
       61 gcttggaatg gaagaggaca tgcccatcga gcaccccttc atttccaaag ctattgagaa
      121 tgcccagacc cgtgttgagg gacacaactt tgagatcaga aaatacctcc ttgaatacga
      181 taacgtgatg aacaagcagc gggaaacaat atatggcatg cggcatgagt tcatgcgaaa
      241 tggcgatgtg cgcgaccaga ttctcgatat gatcgacgag ttgtgtgaaa acatcgtttt
      301 tgactgtgcc ccggaaaagg tctatccaga agaatgggac atggtttccc tcaaaaaccg
      361 gatttttgag accttctttt ttcatatcga tcttgactct gtggacctga agaagctgac
      421 gcgagaaggc ctcctcgatg tggtgagaga aaaggttctt gctgtgtatg aacgtaagga
      481 taaggaattc ggtgatggag aactgaaggc aatcgaacgc tttatcgccc tgaactcggt
      541 tgatgtccac tggaaggaac atctccttgc actggaccac ctgaaggagg ggattggcct
      601 tagaggctat gcacagaaag atcctctccg tgaataccag cgggaaagct ttgacctttt
      661 tctcgacatg atggaaaggg ttaaagttga tactgtacaa aagctctatg ctgttcaccc
      721 tgcccgggaa gaacttaccc gtgagcagcc ggccatgttc tttaacattg gcggtgaaca
      781 accacaggaa aagaaagaca aaaagatcgg caggaatgat ccctgtccgt gtgggagcgg
      841 caaaaaatat aagaagtgtt gcgggagatg acgggggctt gtatgattcc aggtatccgg
      901 tttgcaggaa ttgcttcagg gataaagaat gcagcgcttg atcttggtct tgtagtattt
      961 gatgagccgg catacataac tgctgtctac acaaaaaaca aggttaagtc tgcccacatt
     1021 ctgtataaca aaagacttcg cagtaacgag gtggaggcgc ttcttgtcaa cagcgggtgc
     1081 gcaaatgcgt gcacaggccc ggaaggggtt cgggacttga aagagttgag tgagaaattg
     1141 acctctcatc ttaaagcagc agaaggcaag atcgtgtttg catcaacagg tgttattgga
     1201 cgaaggctgc caacagagac tatggcaaag gccctgcctg ttgctgttaa agacttgaga
     1261 aaaaacggga ttgactcatt ttcccgggca atcatgacga ctgacaccta cccaaagata
     1321 gcccgagcat ccttcaaggg ggagaaagaa tatagcattg ctggtgttgc caagggtgca
     1381 gggatgatca atcccctcct tgccaccatg cttgttttca tcttcaccga ttatcctgcc
     1441 tcacctgaga agatacggcg aaaactcctt cagggcgcta aagagagctt tgaacggata
     1501 accgtggacg gcgaatgcag tacaaatgat tcggtcatgc ttttcacccg ccagggcagg
     1561 gaagacccgg gcagccttga tgctttttgc aatgcccttt tctctgttat gaaagaactg
     1621 gctatgatga ttgtgaggga cggcgaaggg gcaacccgtg ttatccatat cactgtcaat
     1681 ggtgcacaga aaaaagatat tgccgagaaa atagcgcgac gaatagctat atctcccctc
     1741 accaagacgg catttttcgg ctgtgatccc aactggggaa ggatcatagc tgcagcaggt
     1801 gacgcggatg ttcctattca gccggccaaa gtagaaatta tccttcaggg ggaggtcctg
     1861 gcgaagaacg gcatagagat gccctttgac gaggataagg tgaagaagct aatggagaag
     1921 aaggacctg
//
LOCUS       DAOOMC010000223         1863 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdus sp. isolate AMR_MDS_2685
            NODE_79220_length_1863_cov_5.753319, whole genome shotgun sequence.
ACCESSION   DAOOMC010000223 DAOOMC010000000
VERSION     DAOOMC010000223.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864760
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdus sp. (wastewater metagenome)
  ORGANISM  Syntrophorhabdus sp.
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae; Syntrophorhabdus.
REFERENCE   1  (bases 1 to 1863)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1863)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,729
            Genes (coding)                    :: 2,708
            CDSs (with protein)               :: 2,708
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 7 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1863
                     /organism="Syntrophorhabdus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_79220_length_1863_cov_5.753319"
                     /isolate="AMR_MDS_2685"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2699756"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            259..1422
                     /locus_tag="PK125_13650"
     CDS             259..1422
                     /locus_tag="PK125_13650"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HPB39189.1"
                     /translation="MGDAKRQRTKLQGVYERESEGRLFQGKPDKCFDITYKVNGRKIW
                     EKVGWLSEGYSQKMASHIRAERLRSIRHGQDLPQEKKQTPYFRDIAKKYLEWAEQNHS
                     RAGQDDKSMYENHLRGFDDKRLNAITSFTLEKFKADLIKKNLAPASVKNILASIRQIF
                     NKAILWSMYDGSNPVKGVKMPTLQNERQRFLSFEEATLLMETLKANKRHVNVHDMALL
                     ALHCGLRASEIFSLRGRDLDFKNGIITIANPKNKTTRHAYMTDAVREMLTVRNPETPD
                     TFVFRDRNGNPVKDVSNAFQRVVDSLKLNDGIEDPRYKIVFHSLRHTFASWLALQGES
                     LLTIKELMGHKTLSMVQRYAHLSPDHKRTATLSLEKAFMENKGRGTLSTEEKS"
BASE COUNT          596 a          395 c          407 g          465 t
ORIGIN      
        1 ttggtattgg agcccccggt gtgactcgaa cacacggcac acggattagg aatctaacat
       61 ataccctttt tctcctcttc acaagaagtc attcgatatc taaatattat ttcatatcga
      121 tagcataacg caatatgggt aattttttta gttgacagtg aatcacctaa aatgcgaaat
      181 acgggtagca atagggtagc agataatttt atttaccggt tcaattcatg gatcattctg
      241 gaagtgacag gtgagagaat gggtgacgcg aaaaggcaga ggacaaaact tcaaggcgta
      301 tatgagaggg aatcggaggg acgccttttc caaggaaagc cggacaagtg cttcgatatt
      361 acctacaagg tcaacggccg taagatttgg gaaaaggtcg gatggctttc tgaaggatac
      421 tctcagaaga tggcttctca catcagagct gaacgacttc gttccataag gcacggacag
      481 gatctaccgc aggaaaagaa gcagaccccc tatttcagag atatcgcaaa aaaatatctg
      541 gaatgggcgg aacaaaacca ttcgagggcg ggacaagatg ataaaagtat gtacgaaaac
      601 cacctcagag gtttcgacga taaacgcttg aatgccataa cgtcgttcac tctcgaaaaa
      661 tttaaagccg acctcattaa gaaaaatctt gcgccagcca gcgtgaaaaa catccttgcc
      721 tctataagac aaatattcaa caaggcaatt ctctggagta tgtatgacgg atcgaatccc
      781 gtaaagggcg tgaaaatgcc aactctccag aacgagagac aaaggtttct gagtttcgag
      841 gaggcaaccc tgttgatgga aactctgaaa gcaaataaaa ggcatgtaaa cgttcatgac
      901 atggctctcc ttgcgctcca ctgcggcctg agggcgtcgg aaatcttctc cttgcggggt
      961 cgtgacctgg atttcaagaa cgggatcatc acaatagcaa acccgaaaaa caagacgacg
     1021 agacatgcct acatgacgga tgccgtccgt gaaatgctca ccgtcaggaa cccggagact
     1081 ccggatacgt ttgttttcag agatcggaac gggaatccag ttaaggatgt ctcaaatgct
     1141 ttccaaaggg tcgtggacag ccttaagctc aacgatggaa ttgaagatcc acgatacaag
     1201 atcgttttcc attcccttcg ccacacgttt gccagttggt tggcgcttca gggtgaaagc
     1261 cttctgacga taaaggaact gatgggtcac aaaacgttga gtatggtaca gcggtatgct
     1321 catttgtcgc cagatcacaa aagaacggca accttgagtc ttgagaaagc tttcatggaa
     1381 aacaagggac ggggcacact atccactgaa gaaaaatcat aatcattgaa aaatattcat
     1441 tgtcccgaaa gaacatctct taaataatat ctatagttat atcaactaga taaagaattc
     1501 tcgccagtaa ttcccctctc cttctcacgc cgacgcccca acttagcgct cgcagtctgc
     1561 tttcatttat atagcaatta tatatcaaat tgctatcaga tggatatata atttgacaaa
     1621 cagaatatta tcattattat ctgtgtattg cattcattat gtgttattta tatccttgac
     1681 atatgtaaaa ttatatactt taatacaaat aaatcatcct gaactgggag gttgaaaaaa
     1741 tgaaagattc tcatggggaa aaatccctaa ctccgaaaga tgctgcctac atttacggtc
     1801 tgtccgaagg tactcttgcc aatctgcgtt acaacaaaaa aggtccgagg tacttcaaag
     1861 tcg
//
LOCUS       DAOOMC010000224         1859 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdus sp. isolate AMR_MDS_2685
            NODE_79489_length_1859_cov_6.542129, whole genome shotgun sequence.
ACCESSION   DAOOMC010000224 DAOOMC010000000
VERSION     DAOOMC010000224.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864760
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdus sp. (wastewater metagenome)
  ORGANISM  Syntrophorhabdus sp.
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae; Syntrophorhabdus.
REFERENCE   1  (bases 1 to 1859)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1859)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,729
            Genes (coding)                    :: 2,708
            CDSs (with protein)               :: 2,708
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 7 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1859
                     /organism="Syntrophorhabdus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_79489_length_1859_cov_6.542129"
                     /isolate="AMR_MDS_2685"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2699756"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            347..811
                     /locus_tag="PK125_13655"
     CDS             347..811
                     /locus_tag="PK125_13655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB39190.1"
                     /translation="MKKIYIILFFLIFVQLCVVFPVSADPRMEINKNFCHFILDPTNT
                     DNEVFVANCRAEITTNIPLAAKEGQVACEGYVASGYGQVTRTIPQEAIPLKPGETLRF
                     TNENSNVPCTMVESNGRAYTSNNWESVIKVEKGKPKGFVKVTYRLFCREGYQ"
     gene            complement(871..1386)
                     /locus_tag="PK125_13660"
     CDS             complement(871..1386)
                     /locus_tag="PK125_13660"
                     /inference="COORDINATES: protein motif:HMM:NF012360.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HPB39191.1"
                     /translation="MIVGFRGKQPKIDPSAFIAKNATIIGDVSIGASANIWFNCIVRG
                     DNNYIRIGKACNIQDSSVLHIVKDFYPLVLDDEVVLGHRVVVHGCHIGRGSLIGIGAI
                     VLDGAEIGEESIIGAGSVVSPKTVIPPRSLAMGTPARVIRPLNDTDIFKIQGITQEYL
                     DLMEIYRKNCD"
     gene            complement(1485..>1859)
                     /locus_tag="PK125_13665"
     CDS             complement(1485..>1859)
                     /locus_tag="PK125_13665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB39192.1"
                     /translation="TPSILGIIIIFVIFSLTIPQIQAETIETDIGLPLYPGSKEIPDY
                     IPVRTGQNHSSFQNITFITSDSFKKVLAFYQERLGKFSTSKPQTSAKSALWSESTAKG
                     YRIVTLIEAEEGTKITITARTW"
BASE COUNT          524 a          369 c          423 g          543 t
ORIGIN      
        1 gtacatctat acccgggcga acagggctgt gtgtccctat ccctctctct ggcacttccc
       61 gtgaccggtg tggccggtga cgagctgtag aaccgttcgc tcctaagctg tctctttata
      121 cactcgattg tttcccaccg ggatgtttga agagtcgctg gtgtggctgt tggatgcgga
      181 atatcaagtg ttttactcaa agataatcag tttggtctgt gtattgacta caatcacaga
      241 cttatcaata cgcttcagta tattggattg ataaaactgc gacgagaaat atggagaccg
      301 gagtaattag tgaatttcaa aataaaaaaa ggaggtgagc aagacaatga aaaaaatata
      361 tataatcctt ttttttctta tttttgtgca attatgtgtg gtttttcctg tatctgcaga
      421 ccctcgcatg gaaatcaaca agaacttttg tcattttatc ctagacccaa caaataccga
      481 taatgaagtc tttgttgcaa actgcagggc cgagattacc acaaatattc cactggcagc
      541 aaaggagggt caggttgcct gtgaaggtta cgttgcaagc ggatatgggc aggtaacgag
      601 aacgattcct caggaggcaa tcccattgaa gccgggtgaa accttaaggt tcacaaacga
      661 aaattcaaat gtaccctgca ccatggttga aagtaatggc agggcatata catcgaacaa
      721 ttgggagagt gtcatcaagg tagaaaaggg caagcccaag gggtttgtca aagtgaccta
      781 tagactgttc tgcagggaag ggtaccaata gcaggtaaag gaactggatg agataaactc
      841 tttcgattga agtctgcccg gatatcctta tcagtcgcag tttttcctgt aaatctccat
      901 gagatcgagg tattcttggg taattccctg aattttaaaa atatcagtat cgttgagtgg
      961 cctgattacc ctggctggag tccccatggc gagtgatctc ggtggaataa ctgtttttgg
     1021 gctcaccact gatccggcac cgataataga ttcttctcca atctcagccc catcgagaac
     1081 gatagctcca atgccgatta gtgatcctct cccaatatga catccgtgaa caaccacgcg
     1141 gtgaccgagc acaacctcat catccaaaac taatggataa aagtccttga caatgtggag
     1201 aacgctggag tcctgaatgt tgcatgcttt accaatacga atgtaattat tgtcacctct
     1261 cacgatacag ttgaaccata tgtttgctga cgctcctata gacacgtcgc caatgatcgt
     1321 ggcatttttt gcaataaagg ctgatggatc tatttttggt tgctttcccc ggaatccaac
     1381 aatcatgctt catgcacctc tcaggtgaga atcattctat gtatgtaaca tatctgaaga
     1441 ggcaatcttt ggcaatggaa aaaagttaaa aaatagttgt ttgtctacca tgttcttgca
     1501 gtaatcgtaa tctttgttcc ttcctcagcc tctatgaggg tgactattcg atatcctttt
     1561 gcggttgact cactccacag ggctgatttt gctgaggttt ggggttttga agttgaaaat
     1621 tttccaagcc tttcctggta aaaggcaagt actttcttaa aactatctga ggtaataaag
     1681 gtaatatttt gaaaggaaga gtggttctga cctgttctta ctggtatgta atctggaatt
     1741 tctttagatc cgggatagag gggaaggcca atatctgttt caatagtttc tgcctgtatt
     1801 tgtgggatgg ttaaagagaa tatgacaaag ataatgatga tacccagtat agaaggtgt
//
LOCUS       DAOOMC010000225         1850 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdus sp. isolate AMR_MDS_2685
            NODE_80074_length_1850_cov_8.667967, whole genome shotgun sequence.
ACCESSION   DAOOMC010000225 DAOOMC010000000
VERSION     DAOOMC010000225.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864760
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdus sp. (wastewater metagenome)
  ORGANISM  Syntrophorhabdus sp.
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae; Syntrophorhabdus.
REFERENCE   1  (bases 1 to 1850)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1850)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,729
            Genes (coding)                    :: 2,708
            CDSs (with protein)               :: 2,708
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 7 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1850
                     /organism="Syntrophorhabdus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_80074_length_1850_cov_8.667967"
                     /isolate="AMR_MDS_2685"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2699756"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..354
                     /locus_tag="PK125_13670"
     CDS             <1..354
                     /locus_tag="PK125_13670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB39193.1"
                     /translation="IGDEVARMLVTPSLFSIARKKGINANIGAGATAIEVHDAFIRIM
                     YCSYLNYAEVERMDNPDFPEPNLKLMDFVVWAADNQREASEMVEVIFELVTGKSLKKE
                     EEGTEKKKKTKNRRT"
     gene            456..692
                     /locus_tag="PK125_13675"
     CDS             456..692
                     /locus_tag="PK125_13675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB39194.1"
                     /translation="MIKAHNDKEYAAWDRVRMLAHNAYMLTATKGTKIKDRKKFMPLA
                     WDEMNKREIKAIPQPASEAEISELNRIANLFMKK"
     gene            812..>1850
                     /locus_tag="PK125_13680"
     CDS             812..>1850
                     /locus_tag="PK125_13680"
                     /inference="COORDINATES: protein motif:HMM:NF018497.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail length tape measure family protein"
                     /protein_id="HPB39195.1"
                     /translation="MMAKAAWLAVGAAVIKFGKEAVKAYNTQEEAVTKLNQAIKNTGG
                     ALGLTSKELQKYASQLQRTTTFGDEATLNAMATMTTFGSVTGNIFKDAIAAAQDMAAF
                     MKTDLNSAVMQLGKALEIPEIGLSMLRRSGIVFTNEQIANIKKLVEEGKKYEAQQLIL
                     IEVQKKFGGAAAEQAKTAQGVWKQAKNALGDYMETLGRGVEQTKGLAVILRDLFAGWA
                     DARNAGGVKMFNATQEIEEYKNYIDATVGSGEFEKRAAQVQEWYKKSLAEGSKIIDLN
                     GNKISKKYAKVYFAALEKYLKDLTDTGEGAAGVMIGAAEEMIEGNTATEESYNGLIGK
                     IDETISKLEEKK"
BASE COUNT          592 a          342 c          540 g          376 t
ORIGIN      
        1 ataggggatg aggtagcgag gatgctcgtt accccttccc tgttttctat tgcccgtaaa
       61 aaaggtatca acgctaacat cggggcagga gcaacggcta tcgaggtaca tgatgctttc
      121 atcaggatca tgtattgctc gtatctcaat tatgcagagg tggagaggat ggataacccc
      181 gactttcccg aaccgaatct caagctgatg gactttgtgg tctgggcggc agacaaccag
      241 cgggaggcaa gcgagatggt ggaggtgatc tttgaattgg taaccggcaa gagtttgaag
      301 aaggaggaag aggggacaga aaaaaaaaag aaaaccaaga accgcaggac atagacctga
      361 tcggggaatg ggagccaatc agggactttc tgatcggtta ttgcggaaga agtgaagagc
      421 aggcatacag gacctcattc gttgaataca gggcattgat aaaggctcac aacgataaag
      481 agtatgccgc ctgggacagg gtaaggatgc tggcacacaa cgcctatatg ctgaccgcca
      541 caaagggaac gaagatcaag gatcgcaaga agttcatgcc ccttgcctgg gatgagatga
      601 acaaacgtga aatcaaggct attccgcagc ctgcaagtga agcggaaatc agcgaactca
      661 atcggattgc aaatctgttc atgaagaaat aatgggtgtt ttaggaaata tatgggtaaa
      721 acttgggctg aaatctgacg acttccgcaa gggaatggat cagagcgaac agagagtatc
      781 caagtttggt caggcaacgc aaaaaatgtc catgatggca aaggccgcat ggctggccgt
      841 tggtgctgct gttatcaagt tcgggaagga ggctgtaaag gcatataata cacaagagga
      901 agcggtaacc aagctgaatc aggcaataaa aaataccgga ggtgcgttag gtcttaccag
      961 taaggagtta cagaaatacg ccagccagtt gcagcgtaca accaccttcg gggatgaggc
     1021 aacgctgaac gccatggcta ccatgaccac cttcgggagt gtcaccggga atatctttaa
     1081 ggacgccatt gcagcggctc aggacatggc ggcatttatg aagaccgatt tgaatagtgc
     1141 ggtaatgcag ttggggaaag cgttagagat tcctgagatc gggctatcca tgttgcgcag
     1201 aagcggaata gtctttacca atgagcagat tgcaaatatt aaaaaactgg ttgaggaggg
     1261 caagaagtac gaggcccagc aacttatttt aatagaggta cagaaaaagt tcggaggggc
     1321 agccgctgaa caggcaaaaa cagcgcaggg tgtttggaag caggctaaaa acgccttggg
     1381 cgactatatg gaaaccttag gccggggtgt agagcagaca aaagggttgg ctgttatttt
     1441 gagagattta tttgctgggt gggctgatgc acgaaatgca ggcggcgtaa agatgttcaa
     1501 cgccacgcag gaaattgaag agtataaaaa ctacatagat gccaccgttg ggagtgggga
     1561 atttgagaaa agggctgcac aagttcagga gtggtacaaa aagtctttgg cagaaggcag
     1621 caagatcata gacctgaacg gtaataaaat ttcaaagaaa tatgcaaagg tctatttcgc
     1681 agcacttgag aaatacctga aggatttaac cgataccggg gagggagccg ctggtgtgat
     1741 gataggtgcg gcagaggaaa tgatagaggg aaacacagcg acagaagaat cctataatgg
     1801 tttgatcggc aagatagatg aaaccatatc caagcttgaa gaaaagaaaa
//
LOCUS       DAOOMC010000226         1810 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdus sp. isolate AMR_MDS_2685
            NODE_82835_length_1810_cov_5.950427, whole genome shotgun sequence.
ACCESSION   DAOOMC010000226 DAOOMC010000000
VERSION     DAOOMC010000226.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864760
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdus sp. (wastewater metagenome)
  ORGANISM  Syntrophorhabdus sp.
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae; Syntrophorhabdus.
REFERENCE   1  (bases 1 to 1810)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1810)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,729
            Genes (coding)                    :: 2,708
            CDSs (with protein)               :: 2,708
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 7 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1810
                     /organism="Syntrophorhabdus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_82835_length_1810_cov_5.950427"
                     /isolate="AMR_MDS_2685"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2699756"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..199)
                     /locus_tag="PK125_13685"
     CDS             complement(<1..199)
                     /locus_tag="PK125_13685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB39196.1"
                     /translation="MKSEQEKLNNILSATTVGLLIMQSIISIIILISIRNLVAQNQLL
                     ISTHNTEEEFQIFGESFSDFEL"
     gene            721..>1810
                     /locus_tag="PK125_13690"
     CDS             721..>1810
                     /locus_tag="PK125_13690"
                     /inference="COORDINATES: protein motif:HMM:NF025063.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integrase core domain-containing protein"
                     /protein_id="HPB39197.1"
                     /translation="MIKDIIRLDYQELRRSSPIAARQAILQILKAHKGNVSQTAQVLN
                     ISRATVYKAIRKKEAGDLHDASRAPKHVHNKTNKKLEERVLEIHKQTGFGPVRIKEEL
                     EEVDDLYLSPHTIRNIIRRNKSKGKKQRSANKTERQFVDWYSAKAFEVVQIDLKYIVD
                     QKALSMEQIHHIHTRNLPRYQWTAIDVNSRFRLMAYSYEKTWTNGLAWFLWVLSWLRS
                     HGVTSHVVFTVDRGEEFGGKSWLKVHELKKLLSDFGCSFVQNRAGHPEENPHIERSHR
                     TDDDEFYIPRILSIDSPKDFFFEAMNYLYYYNVHRRHSSLGRLPPYSYLSKSVTGLDD
                     KIKFIPPIFLDYLAVQLGDWCGYHLLASH"
BASE COUNT          525 a          401 c          391 g          493 t
ORIGIN      
        1 ttagttcaaa atcagaaaag gattccccaa aaatctgaaa ttcttcttct gtgttatggg
       61 ttgagatgag tagttggttt tgagctacaa gattacgtat tgaaattaat attattatgg
      121 aaatgatact ctgcatgatg agtaacccaa ctgttgtggc agaaaggata ttgtttagtt
      181 tttcttgttc agatttcatg ttgcttccta gtttaaagtt taaaagtgaa ttctcaaaag
      241 gtgaaacata tttattttga taatctccaa aaatcgattc aagcttgatg tgcgggggtg
      301 acacagcagt catcacgcta tgtgtccaga gaataatctc tccacctcag aggagtagaa
      361 attccctggt ggtaagaaaa tcattgtcaa aacccatagg cgagatatat ctataaaatc
      421 ttcttccgcg taattgaatt agaaaatcaa tttcattcct cctattctga tcgaattgaa
      481 tatatttaga acagaaatcc taggctctgc tagcttccgc tagatcgccg ataatttatt
      541 gctgatattc acctcctctg taattataca ttttactgcg cttacataaa aagcacatgt
      601 tcgacaatat tgtgcgtggg tcagcacata gtatacactt tatgaatcga gggtatgaga
      661 gataccgcac caggtgtctg gagctttggg gattaccata tgaggacatt gaggtgcaaa
      721 ttgataaagg atatcatccg cttagattac caagagttga gacgaagcag cccgatagca
      781 gccagacaag cgatcctgca gatactcaag gcccataaag gcaacgtgag ccaaaccgct
      841 caggtattga acattagtcg ggcaaccgtc tataaagcta tcaggaagaa agaggcagga
      901 gatctgcatg acgcctccag agcgccaaaa cacgtgcata acaagaccaa caagaagctt
      961 gaagaacgag tcctggaaat ccacaaacaa acagggtttg gtcctgtgcg gatcaaagaa
     1021 gagctggagg aagtagatga tctctatctg tccccacaca ccatccgcaa catcatcaga
     1081 cgcaataagt ctaagggaaa gaaacagcgc tcagccaata aaaccgagcg tcagtttgtc
     1141 gactggtact cggccaaagc ctttgaagtg gtccagattg acctgaagta tatcgtcgac
     1201 caaaaagcgc tctccatgga acagatccat cacatccaca ccaggaattt accccgctat
     1261 caatggactg ccattgatgt gaactcccgt ttcaggctga tggcatattc ctatgagaag
     1321 acctggacca acggtttggc ctggttttta tgggttcttt cctggctgag aagccatggc
     1381 gtgacttccc atgtcgtttt tactgtcgac cgcggtgagg agtttggggg gaaatcctgg
     1441 ttgaaagtgc acgaactcaa aaaactgctg tctgacttcg gttgttcctt tgtccagaac
     1501 agggccggac atcctgagga gaatcctcat atcgaacgct ctcatcggac cgatgacgat
     1561 gagttctata tcccccgcat tctctcgatt gattcaccca aagatttctt ctttgaagca
     1621 atgaattatc tgtattacta caatgtgcac aggcggcact cttctctcgg tcgcctgccc
     1681 ccttacagct atttatctaa gtcagtcacg ggtttggatg ataaaatcaa attcattcca
     1741 cccatcttcc ttgactatct cgcggttcaa ctcggcgatt ggtgtggata ccatctgctg
     1801 gcttctcacc
//
LOCUS       DAOOMC010000227         1810 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdus sp. isolate AMR_MDS_2685
            NODE_82850_length_1810_cov_4.335043, whole genome shotgun sequence.
ACCESSION   DAOOMC010000227 DAOOMC010000000
VERSION     DAOOMC010000227.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864760
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdus sp. (wastewater metagenome)
  ORGANISM  Syntrophorhabdus sp.
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae; Syntrophorhabdus.
REFERENCE   1  (bases 1 to 1810)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1810)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,729
            Genes (coding)                    :: 2,708
            CDSs (with protein)               :: 2,708
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 7 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1810
                     /organism="Syntrophorhabdus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_82850_length_1810_cov_4.335043"
                     /isolate="AMR_MDS_2685"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2699756"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..215)
                     /locus_tag="PK125_13695"
     CDS             complement(<1..215)
                     /locus_tag="PK125_13695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB39198.1"
                     /translation="MIDYRKELAEIEKLINENRYEECSMLCGKRIELVLKELAKDYLK
                     KVSETSQRAFKENIRQGQPLERMTLRPL"
     gene            712..1206
                     /locus_tag="PK125_13700"
     CDS             712..1206
                     /locus_tag="PK125_13700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HPB39199.1"
                     /translation="MGTTHWFFGCATEQQIKKRFHELMLIHHPDRGGNLQICQEIVGQ
                     YHDALKSCLYTGCEYNGEKQAYRYNEAMEKEFEEAMDWAMTLSFCKVELIGTWLWVSG
                     KTFPISNKFRERGFHWASKKHAWCFHVGLWRRNKRHFALDDLRAKYGYEVLKEEEDQK
                     AMTC"
     gene            1254..1532
                     /locus_tag="PK125_13705"
     CDS             1254..1532
                     /locus_tag="PK125_13705"
                     /inference="COORDINATES: protein motif:HMM:NF015935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="JAB domain-containing protein"
                     /protein_id="HPB39200.1"
                     /translation="MLVQFKEDNVGIRNDGNVADLLQSILKTESPIDRDKEHFWTIGL
                     NTRNVVKYVDLTSLGTLNASLVHPREVFRLAIMEGVAGIVRCFKKTRF"
BASE COUNT          534 a          370 c          415 g          491 t
ORIGIN      
        1 aggggacgaa gggtcattct ctccagtggc tgaccctgcc gaatgttttc cttgaaagct
       61 cgttgagagg tttcagatac cttttttagg taatctttgg ccagttcctt tagaacaagc
      121 tcgatgcgct tgccacacag catggagcat tcctcgtacc gattctcatt gataagcttc
      181 tcaatctcag ccaattcttt acgataatcg atcatcggtt acctcatggg cgacgcttca
      241 tagaaattag aatgtttagt tttttgtaat attttcctcg atgtttttca atctttttct
      301 gcatgtagca taatttgtat attaattttg tgcaagctgt tcactgctaa acccacgtgc
      361 atatattgta tacttttttg ccaatagact caggcttctt ctataacgat agttatacat
      421 agggttccct acaagaataa acggcagggg ggtgattttc gcttccgggt ggtaccccct
      481 ctccagaaaa aagaatgctt ttctaccctc aaaaaggaac gaccaggagc cagaagaatt
      541 catgaaaaga ttaggcctcc atcacacacc caatcaaccc cggcccatga acctcgtttg
      601 acagaatacc caatgattat ggtatattag accatacatg acaatcgaat atgactatga
      661 aggcatccca tccggatgcc ttttttattc caagaaaagg aggttttact tatgggaaca
      721 acacactggt tcttcggatg tgcaacagag caacagatca agaagaggtt tcatgaattg
      781 atgttgatcc accacccgga caggggaggg aatctccaga tatgccagga gattgtgggg
      841 caataccatg atgcattgaa atcctgcctg tataccggat gtgagtataa cggggagaag
      901 caggcatacc gttacaacga agccatggaa aaggaatttg aagaggccat ggactgggcc
      961 atgacgttgt cattctgcaa ggttgaattg attggaacat ggctctgggt atcgggcaaa
     1021 accttcccca tcagcaacaa gttccgagaa agaggcttcc actgggcaag taaaaaacac
     1081 gcctggtgct tccatgtggg gttatggaga agaaataaga gacactttgc cttggatgat
     1141 ctccgggcaa agtacggcta tgaggtctta aaggaagaag aagaccagaa ggcaatgacc
     1201 tgttaagggg aaggggaacc ttctcctttt tctttcatca taaggagggc gctatgttag
     1261 ttcaatttaa ggaagacaat gttggcatac ggaacgatgg aaatgttgca gatctcctgc
     1321 agtccatctt gaaaacagag tcacccattg atcgggacaa agagcacttc tggacaatcg
     1381 ggctcaatac caggaatgtg gtgaagtacg ttgatcttac gagcttgggc accctcaatg
     1441 cttctttggt ccatccccgt gaggtattca ggctagcaat catggagggc gttgcaggca
     1501 tagtccggtg ctttaaaaaa acaaggtttt gaattatttc atcaagacta cagagttaaa
     1561 agaatatcaa agggaatggt atgctatcct tggactgaaa tagcagtttt gacaaagcat
     1621 ctaccaatca tctaatgcac aataacattt ggtagttctg tcaataaaag attaccttga
     1681 ctattcacct gtagagtgta aaaattgaaa ataattcaac tcattgggta tggctaattt
     1741 aaatcaatgc tatgagacct tggggcttgc ataatggggc ttcccttgaa gaagtgaagc
     1801 aggcgtacaa
//
LOCUS       DAOOMC010000228         1778 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdus sp. isolate AMR_MDS_2685
            NODE_85118_length_1778_cov_5.077771, whole genome shotgun sequence.
ACCESSION   DAOOMC010000228 DAOOMC010000000
VERSION     DAOOMC010000228.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864760
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdus sp. (wastewater metagenome)
  ORGANISM  Syntrophorhabdus sp.
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae; Syntrophorhabdus.
REFERENCE   1  (bases 1 to 1778)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1778)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,729
            Genes (coding)                    :: 2,708
            CDSs (with protein)               :: 2,708
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 7 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1778
                     /organism="Syntrophorhabdus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_85118_length_1778_cov_5.077771"
                     /isolate="AMR_MDS_2685"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2699756"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            224..664
                     /gene="mraZ"
                     /locus_tag="PK125_13710"
     CDS             224..664
                     /gene="mraZ"
                     /locus_tag="PK125_13710"
                     /inference="COORDINATES: protein motif:HMM:TIGR00242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0051302 - regulation of cell division
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HPB39201.1"
                     /translation="MFAGRYEYAIDDKSRVSIPAKFREALSTNYDMRLILTNLDGCIV
                     GYPYQEWLSIQERTSSPGAIRKETRTFLRYFYSGVSECPIDRLGRILIPQALKTDAQV
                     KKNVVIIGMGKKIEIWAQERWDELVKKATADPDQIADIVSELGL"
     gene            664..1530
                     /gene="rsmH"
                     /locus_tag="PK125_13715"
     CDS             664..1530
                     /gene="rsmH"
                     /locus_tag="PK125_13715"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: protein motif:HMM:NF013919.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HPB39202.1"
                     /translation="MGVTHIPVLREEVADNLASNTGRLFIDATVGGGGHSSFILTRYP
                     DLNLLGLDVDEGNLKTAEARLREFGGRVTLIRGNFRNLKAILQQAGISSFDGILFDLG
                     LSTYQLTGARGFSFHDESFLDMRMDGREALTAYDVVNKYNYEALKRVILEFGEEYRAG
                     RIAKVITEERKKKPITTAKELAEIVGKAKKRTGKIHPATKTFQAIRIEVNEELNSLRS
                     GLADAIDMARPKGRIGVISFHSLEDRIVKEMFRGSASLSIVTKKPIRPGREEVLSNPN
                     ARSAKLRVAEKK"
     gene            1556..>1778
                     /locus_tag="PK125_13720"
     CDS             1556..>1778
                     /locus_tag="PK125_13720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB39203.1"
                     /translation="MEKVYVTVKKNAQQRNAKHLLTIILLSAIFIIVTLHMRGEYNGI
                     REELIESLKRERAVTEVNNKLKMELSVITR"
BASE COUNT          567 a          316 c          449 g          446 t
ORIGIN      
        1 ttgtctttcc ggcatcgata tgtgctgcaa tccctatatt tcttatcttt ctgagcctca
       61 aggcctcttt tttcataacc ttaagatata gaactattac cctgtaaaag tcaatattaa
      121 tggggttaaa gattcttttc ataagtataa aaaaatgctt gacaaacctt ggtgctgcga
      181 gtataaatgt gaaaagtggt aaaaggtgtt ataaggtggg ataatgttcg ctggtaggta
      241 tgagtacgcc atcgatgata agagcagagt gagcattccg gcaaagttca gagaggccct
      301 ctctacgaac tatgacatga ggcttattct tacaaacctc gatggctgca ttgttggata
      361 cccctaccag gaatggcttt ccatacaaga aagaacatca agcccagggg caatcagaaa
      421 ggaaacacgt acatttctca ggtattttta ttccggagtc tctgaatgcc ctatagacag
      481 acttggaaga attctcattc ctcaggcgct aaagaccgat gcacaggtaa agaagaacgt
      541 tgtgatcatt ggcatgggaa agaaaataga gatatgggcg caggagcgtt gggatgaact
      601 ggtaaagaag gcaactgctg accctgatca gatagctgac attgtttctg agcttggtct
      661 ttgatgggtg ttacgcatat acctgttctt cgcgaagaag tagcagacaa tctggcaagc
      721 aatactggca ggttattcat agatgcaaca gtcggtgggg gtggccattc atctttcatt
      781 ttgacaagat atcctgacct gaatttatta ggtttggatg tggatgaggg caatctgaaa
      841 acagctgagg cgagactaag ggaatttggt ggtagagtaa cattgattag gggaaatttc
      901 agaaatctaa aagcgatcct gcaacaggca ggcatttctt cttttgatgg catcctcttt
      961 gatcttgggc tttcaacata tcagcttacc ggagcaaggg gttttagctt tcatgatgag
     1021 tctttcctcg acatgagaat ggatgggaga gaagccctga cagcttacga tgttgtaaat
     1081 aaatacaact atgaagccct gaagagggta atcctggaat ttggtgaaga atacagagcg
     1141 ggcaggatag caaaagtgat aaccgaagaa agaaagaaaa aaccaataac cacagcaaaa
     1201 gagctggcag aaattgttgg gaaggcaaaa aaaagaacgg gcaaaattca ccctgccacg
     1261 aaaacctttc aggcaatcag gattgaggtc aatgaagagc ttaatagcct tcgatccggt
     1321 ttggcagatg caatagacat ggcaagaccg aagggaagga ttggcgtcat atccttccac
     1381 tctctcgaag acaggattgt caaagaaatg ttccgggggt cggcatcgtt gagtattgtt
     1441 acaaaaaagc ccataaggcc gggaagggag gaagttttat caaacccgaa cgcaaggagc
     1501 gctaagctca gggtagctga aaaaaaatag tctgaggggg ttgtaagggg gcactatgga
     1561 aaaggtgtac gtaacagtta agaaaaatgc gcaacaacgg aatgcaaaac atcttctgac
     1621 cataattctc ctgagcgcta ttttcattat tgttactctc catatgcggg gagaatataa
     1681 cggtatcagg gaagaattga tagaaagttt gaaacgggaa cgggcagtta ctgaggtgaa
     1741 caacaaattg aaaatggagt tgtcagtaat taccagag
//
LOCUS       DAOOMC010000229         1773 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdus sp. isolate AMR_MDS_2685
            NODE_85497_length_1773_cov_4.218859, whole genome shotgun sequence.
ACCESSION   DAOOMC010000229 DAOOMC010000000
VERSION     DAOOMC010000229.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864760
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdus sp. (wastewater metagenome)
  ORGANISM  Syntrophorhabdus sp.
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae; Syntrophorhabdus.
REFERENCE   1  (bases 1 to 1773)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1773)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,729
            Genes (coding)                    :: 2,708
            CDSs (with protein)               :: 2,708
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 7 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1773
                     /organism="Syntrophorhabdus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_85497_length_1773_cov_4.218859"
                     /isolate="AMR_MDS_2685"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2699756"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..253)
                     /locus_tag="PK125_13725"
     CDS             complement(<1..253)
                     /locus_tag="PK125_13725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB39204.1"
                     /translation="MTQVHIRFSDHQVKEFIERYIHHEIDQIYIQQMLGMGKSRFFIL
                     LQRCREDPEGFSIAYTRHKKTRGIPPLIEGHILEELAVDK"
     gene            complement(1177..1470)
                     /locus_tag="PK125_13730"
     CDS             complement(1177..1470)
                     /locus_tag="PK125_13730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB39205.1"
                     /translation="MCKKNSQFKPTYQYIEYHFEPSELIHHAPDDQSLTLAKTHNGPI
                     IIGLVLAFSVSFALSTKKTAYVLRSVFGIPISYQSVLNYIMVAVFPVILQSEP"
     gene            1562..>1773
                     /locus_tag="PK125_13735"
     CDS             1562..>1773
                     /locus_tag="PK125_13735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB39206.1"
                     /translation="MSLDAEDFIVDFAQKLNPFLSIIVIEILMGSMAERTKDELKNTD
                     RTCSISHRKLGLAVVLPILCKKFFVV"
BASE COUNT          505 a          345 c          408 g          515 t
ORIGIN      
        1 ctttatcaac agcgagttct tcgaggatgt ggccctcaat gagaggaggg atacccctgg
       61 ttttcttatg cctggtgtag gcaatggaaa acccctcggg gtcttcccgg catctctgca
      121 aaagtataaa gaacctggat ttccccatac ccagcatctg ctgaatatag atttggtcta
      181 tttcatggtg gatgtaccgc tcaatgaact ctttgacctg atgatcagaa aaacgtatgt
      241 gtacctgtgt catactcaaa aacctcttta tatgtttttg agtaatctgt accccaggtg
      301 tccactttta aatcttaaat gacaatatac cgatacaggt tgacatctca gtaaaaggtg
      361 ctatatcaaa ataagagaac agcataatag gactcgcaat cggtattatc gaaactttta
      421 atgaagggct gtgaatttaa tatgaaatta ctcacactaa aggtaataag gaagataaac
      481 agggagggcc gtataaagag atggcaatag gattgttcag cctgtcttat aaggcccaaa
      541 ccacgaaaca aaagcactta ggttttattg ttctttgaca attgaagaga ggtaatcctc
      601 tttatgcaac aatggagagg attattatcc acttgcattg tatggtggcg attccatcca
      661 attcattcag agggatggtc gcctgtcctt tgaaagaagt atgggacctc aggaaattat
      721 aatgggtaac aaacaggatg acgagagcta ttgcaccgtt tagctgttta acccatgtga
      781 tggtttcaca taatgcttga agatcctatt caaccattca atgagctgtt tgaatgtctg
      841 gtaagcttcc gactcgcgat ctaagttctt aaacccgatg actttcttgt ggcaaattaa
      901 cacttctctc ctttcgctgg agaagtgcat acctacgtgg atgccaacag ggggcgaagg
      961 attcccgttg gtcacgatgg ctatcggctg actttgatct gccgtcctga tcgcctcctt
     1021 catggcaata atgccgtgca ctgtctccct ggtatcgaca agatgatatg cggtgatctt
     1081 cagatttttt gaagagatga agaagaaaca tagtgctgtt ttccctcgat tttgatatac
     1141 accacatcgc cagcactgat ctgatcgatt agttctttat ggttcagatt gaagaataac
     1201 aggaaaaaca gctaccataa tatagttcaa tacagactgg tatgaaatgg gaatgccgaa
     1261 gactgatcga agaacatacg cggtcttctt ggtagaaagg gcaaaggaga cggagaaggc
     1321 aagaacaagg ccgatgatta taggcccgtt gtgggttttt gcgagagtga gggactgatc
     1381 atcaggtgca tgatggatga gttcggaagg ttcgaagtga tattctatgt actggtaggt
     1441 gggcttgaac tgggagtttt tcttacacaa gctgatattc ccgttcattt agcttatttg
     1501 tctgtcgtat ctgatatacg catgcatcat tgcagcattc gtagaagatc ttcttctgga
     1561 attgtctctc gacgcggaag atttcattgt agattttgca cagaagctga atccttttct
     1621 ttccatcatt gtaatagaga tactcatggg gagcatggca gagaggacaa aggacgaact
     1681 caagaacacg gacagaacct gttcgatatc tcaccgaaaa ttaggtcttg ccgtagttct
     1741 gcccatactt tgcaagaagt tctttgtcgt ttt
//
LOCUS       DAOOMC010000230         1761 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdus sp. isolate AMR_MDS_2685
            NODE_86386_length_1761_cov_6.739742, whole genome shotgun sequence.
ACCESSION   DAOOMC010000230 DAOOMC010000000
VERSION     DAOOMC010000230.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864760
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdus sp. (wastewater metagenome)
  ORGANISM  Syntrophorhabdus sp.
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae; Syntrophorhabdus.
REFERENCE   1  (bases 1 to 1761)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1761)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,729
            Genes (coding)                    :: 2,708
            CDSs (with protein)               :: 2,708
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 7 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1761
                     /organism="Syntrophorhabdus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_86386_length_1761_cov_6.739742"
                     /isolate="AMR_MDS_2685"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2699756"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..832)
                     /locus_tag="PK125_13740"
     CDS             complement(<1..832)
                     /locus_tag="PK125_13740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB39207.1"
                     /translation="MTNKKSPVVYVDHTVLSLVPKQVPDSDPAFEDWFASQELWREFR
                     EEKIKLVTHGKDTEMDIILWLNRQGCCITDTLRAVEAINEFEAWNKIEKSHIQQYKQM
                     LIHFEEIESIHPPQGRFEEHSTKDDITKVLRLKPMGADNAESTEGDQNLLRQCLSEVG
                     NWYIEDRWKDLKRTDYQLNWQILESVLIRQGVEPVFHGVEGDRNRNLFGLLNRAVGLT
                     KKSCGRLPVPDTHINFVINMVLQKYSHDQVLSGISHLLHCIMHNINFYVTVNHRLIQG
                     F"
     gene            complement(923..1264)
                     /locus_tag="PK125_13745"
     CDS             complement(923..1264)
                     /locus_tag="PK125_13745"
                     /inference="COORDINATES: protein motif:HMM:NF015953.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HPB39208.1"
                     /translation="MLLILKWLIMTVSVMVTAYVIPGVTVRSFMSALWVALLLGIVNM
                     LLRPFLILITLPINILTLGLFTFVINGLIILLVSSVVKGFQVSGFWIAVLFSIVLSIV
                     NYVMNFFIGTR"
     gene            complement(1383..1667)
                     /locus_tag="PK125_13750"
     CDS             complement(1383..1667)
                     /locus_tag="PK125_13750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB39209.1"
                     /translation="MIDEIKTVDDLLKAKKVTPEERELLKDIIEVARTNERKIREYAE
                     QMKANFNRLSQALQTMEERTFILNKTLQGLLDATDTLQLRLMPSDKFYRE"
BASE COUNT          477 a          396 c          337 g          551 t
ORIGIN      
        1 tgaaaccctg tattagcctg tgattaactg tgacataaaa attgatgttg tgcataatac
       61 agtgcaaaag atggcttatg ccgcttaata cctggtcatg gctatacttc tgaagcacca
      121 tgttgatcac gaaattgatg tgagtatccg gtacagggag tcttccgcaa cttttttttg
      181 tgagtcctac tgctcggttc aataatccaa agagattgcg gtttcgatct ccttcaacac
      241 catgaaaaac cggttcaacc ccttgcctga taagtaccga ctcgaggatt tgccaattca
      301 attgataatc cgttctcttc aagtccttcc agcgatcttc gatgtaccag ttgccaactt
      361 cggagagaca ttgcctcagg aggttttgat ctccttcagt ggattcggca ttatccgccc
      421 ccatgggttt gagcctcaag acttttgtaa tgtcatcctt tgttgaatgt tcctcaaacc
      481 ttccttgagg tgggtgaatc gattctatct cttcaaaatg aatgagcatt tgcttgtatt
      541 gttgaatatg acttttttca atcttgttcc aggcttcaaa ttcattgatt gcctcaactg
      601 ccctgagagt atcggtaata cagcatcctt gcctgttgag ccagagaatg atgtccattt
      661 ctgtatcttt tccgtgggtt acaagtttta tcttttcttc tctgaattct ctccagagtt
      721 cttgagacgc aaaccagtcc tcaaaggcag ggtcagaatc gggcacctgc ttaggtacaa
      781 gactcaagac tgtgtggtca acataaacta ccgggctttt cttattggtc acaatatgcc
      841 tccaaactca gtgaacccat aatcggggta ttctgaaaca aaatcatttt tcaggccacc
      901 aaagcctata gatctttgaa agctatcgcg ttcctatgaa gaagttcatc acgtagttga
      961 caattgacaa cacaatggag aacaggactg caatccagaa accgctcact tggaaacctt
     1021 ttactaccga tgatacaaga aggataataa gcccattgat tacaaaggta aaaagcccca
     1081 atgtcaggat attaatagga agtgttatga gaatgagaaa tggtctcagg agcatattga
     1141 ctataccgag taacaatgca acccacaagg ctgacatgaa actgcgcact gtaacccctg
     1201 gtatcacgta ggctgtaacc attacagaaa cagtcattat tagccacttt aaaataagaa
     1261 gcacctgcgt tctccttttg ggaaaagact aacccatctc ataatcatta ttaattttgc
     1321 tgtaaaggaa agatgttacg aaactagaac aatacgctac gtggttatat ctatttattt
     1381 ttctactcgc ggtaaaactt gtcacttggc atcaatctca gttgaagcgt atccgttgcg
     1441 tcaagcaaac cttgtaaggt cttgttcaag atgaatgtcc tctcttccat agtctgtaaa
     1501 gcctgggaca atctgttgaa gttcgccttc atctgttcgg cgtattcccg aattttccgt
     1561 tcgttggttc ttgcaacctc gatgatatct ttcaggagct cacgctcttc aggggttact
     1621 ttttttgcct ttagaagatc atccaccgtc ttaatttcat ctatcatggc tttctccaca
     1681 gattcatttc caggatatag ttacactatt gtacccttcc tggtgaattc ttgcaagccc
     1741 aatcttcttg gtttttcttc a
//
LOCUS       DAOOMC010000231         1751 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdus sp. isolate AMR_MDS_2685
            NODE_87171_length_1751_cov_5.416274, whole genome shotgun sequence.
ACCESSION   DAOOMC010000231 DAOOMC010000000
VERSION     DAOOMC010000231.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864760
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdus sp. (wastewater metagenome)
  ORGANISM  Syntrophorhabdus sp.
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae; Syntrophorhabdus.
REFERENCE   1  (bases 1 to 1751)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1751)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,729
            Genes (coding)                    :: 2,708
            CDSs (with protein)               :: 2,708
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 7 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1751
                     /organism="Syntrophorhabdus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_87171_length_1751_cov_5.416274"
                     /isolate="AMR_MDS_2685"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2699756"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(102..1616)
                     /locus_tag="PK125_13755"
     CDS             complement(102..1616)
                     /locus_tag="PK125_13755"
                     /inference="COORDINATES: protein motif:HMM:NF033551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182 family transposase"
                     /protein_id="HPB39210.1"
                     /translation="MDFRSNDRDQQVFFGYSLSDFVPANSFPAFVVQFVSELNIDPII
                     ESYSRDGSKAYDPRIMLSIWFLAYSYGIKSTRRVEYECKKNLEFIYVSANLKPDHTTL
                     HRFRVLVKNLMPNLFTDVVMNIVQQTNIKFDSIAVDGTKMRSRSSWRKSRTEKEIEAM
                     LAKVREDIAEYLSEVDKDEEECEKLKKKEASLEEAREKIVKRKQTIEKEHKHTHKINL
                     VEEDAYSMRFPSGKDAFPGYNAMVSACTETQLIVAAEVTQRRSDHGEFSRQHKNIESV
                     IGSDKSRKYLVDSGFYTRDEANYALENGVDLYFPEIWHYGRSGKEIGKKQLQYDEVRD
                     LYVCPEGKELLPQRHEGEFIGYITKECKNCPLREKCTSTREGDRYVKKVLRHKSEKEF
                     ESLVLRGRTPEGKKMVELRQRTVEPVIGNLKSNLGFNRFSCFGLEKVRAEFAFMCIGH
                     NLRKLYKMFLRGDFPFPGGFFDRFILLFTKILWVNRPSGKSWTILPILKPIFRL"
BASE COUNT          474 a          421 c          322 g          534 t
ORIGIN      
        1 caggggttta acaggggtag aagaacattt atcaaaaagc cctaaccccg tcaggaggcc
       61 gttgcacaac tattttttga aaaactacaa gaaacccggt tttacaaccg aaagattggc
      121 tttagaattg gcagtatcgt ccaacttttt cccgatggtc tgttcaccca cagaattttc
      181 gtaaacaaaa ggataaatcg atcaaaaaag ccccccggga agggaaaatc ccccctcaag
      241 aacattttat acaacttacg aagattatgg cctatacaca tgaacgcaaa ctctgctctg
      301 accttctcca acccaaagca actgaacctg ttgaacccaa ggtttgattt aagattcccg
      361 atcaccggtt caacagttct ctgtctcagt tcaaccatct ttttcccctc tggtgttcta
      421 ccccttaaaa ccaacgactc aaattccttt tcgcttttgt gccggagaac ttttttgaca
      481 tatctgtcac cctccctcgt tgatgtacat ttttctctca gtggacagtt tttacactcc
      541 tttgttatat aacctataaa ttcaccttca tgcctctgcg gtaacaactc tttcccctcg
      601 ggacacacat acaggtctcg gacctcatca tattgtaatt gctttttccc aatctccttc
      661 ccacttcttc cgtaatgcca aatttcaggg aaatacagat cgaccccatt ctcgagcgca
      721 tagtttgcct catcccgggt atagaaaccc gaatcaacca aatatttcct gcttttatct
      781 gaacctataa cggactctat attcttgtgc tgcctgctga attcaccatg atcagacctc
      841 ctttgggtaa cttccgcggc tactatgagt tgtgtctctg tgcaagctga taccattgca
      901 ttataaccag ggaaagcatc cttccccgat ggaaatcgca tcgagtaggc atcctcttcc
      961 actaaattta ttttgtgagt atgtttgtgt tccttctcaa tggtctgttt tctcttaact
     1021 attttctctc tggcttcctc cagggatgct tctttctttt ttaacttctc acactcctct
     1081 tcgtctttat cgacttcgga caaatattcg gcgatatcct ccctgacttt tgcaagcatc
     1141 gcctcaattt ccttttctgt gcgagattta cgccaactgc tccgtgatct cattttggta
     1201 ccatcaacgg cgatggaatc gaacttgata ttggtttgtt ggacgatgtt cattacaaca
     1261 tcggtgaaaa gattgggcat cagattctta acaagaacac ggaatctgtg gagggtagtg
     1321 tgatccggtt taaggttagc ggagacatag ataaattcaa ggtttttctt gcactcatat
     1381 tccacccttc tggtggattt tatgccgtaa ctgtaagcca ggaaccagat tgaaagcatg
     1441 atccttggat cataggcttt acttccatcg cgggagtagg actctatgat gggatctata
     1501 ttaagctcgg aaacaaattg aaccacgaag gcagggaagg agttagcggg gacaaagtca
     1561 ctgagggaat agccaaagaa gacttgttgg tcacgatcat ttgatctgaa atccattaaa
     1621 aaaccttgaa attgttgtta taatataact ataatatgaa tagctatata atatatatgc
     1681 tattctgcaa cagcctccgt caggggttta acaggggtag aagaacattt atcaaaaagc
     1741 cctaaccccg t
//
LOCUS       DAOOMC010000232         1729 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdus sp. isolate AMR_MDS_2685
            NODE_88855_length_1729_cov_5.767622, whole genome shotgun sequence.
ACCESSION   DAOOMC010000232 DAOOMC010000000
VERSION     DAOOMC010000232.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864760
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdus sp. (wastewater metagenome)
  ORGANISM  Syntrophorhabdus sp.
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae; Syntrophorhabdus.
REFERENCE   1  (bases 1 to 1729)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1729)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,729
            Genes (coding)                    :: 2,708
            CDSs (with protein)               :: 2,708
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 7 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1729
                     /organism="Syntrophorhabdus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_88855_length_1729_cov_5.767622"
                     /isolate="AMR_MDS_2685"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2699756"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            38..850
                     /locus_tag="PK125_13760"
     CDS             38..850
                     /locus_tag="PK125_13760"
                     /inference="COORDINATES: protein motif:HMM:NF013068.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HPB39211.1"
                     /translation="MTAKWYKSARAFGVSPSSDIIAVTAKNLTVFKERDLGDLTIFAL
                     FINTIHRGGEAYMVALGIDTEGVKHVLGFWQGVTENHEVCTGLHADLERRRLTLSKNI
                     LWITDGGRGIIKTWSTTHKDRNIQGHLAKKYRKEAHRRFTTALEQNDYKDAREMLLDF
                     EKWLRAINESAADSLMEAIEGILTLHRLNIPVLIRKTLTSTNPIESMFSTVRDCERNI
                     KRYRTSAMAQQWLASVLLHCEKGFRRIKGYRDIPQVLANIEAIHTEATRQAA"
BASE COUNT          492 a          400 c          353 g          484 t
ORIGIN      
        1 gaccctcgtg gggtttcact gggccattat ttaattattg acggcgaagt ggtataagtc
       61 cgcccgtgcc ttcggagtct ctccttcttc ggatatcatt gcggtcactg cgaaaaacct
      121 caccgtgttc aaagaacggg acctcggaga cctcaccatc tttgccctct ttatcaatac
      181 gatccatcgg ggaggagaag catacatggt tgccctgggc atcgacacag agggagtcaa
      241 gcacgttctc gggttctggc agggcgttac ggaaaaccat gaggtctgta ccgggcttca
      301 tgccgatctg gagagacgac gccttaccct ttcaaaaaac atcctctgga taacagatgg
      361 aggcaggggg attatcaaga cgtggtccac cactcataag gacagaaaca tccaggggca
      421 tctggcaaag aagtaccgga aggaagccca ccggagattc accactgccc tggaacagaa
      481 cgactataag gatgcacggg agatgctcct tgatttcgag aaatggcttc gtgctatcaa
      541 tgaatcagca gccgattctc tcatggaggc catcgaagga atccttaccc ttcaccgatt
      601 gaacatacct gtccttatca gaaaaaccct tacctctacc aatcccattg aaagcatgtt
      661 ctccactgta cgggattgtg agcggaacat aaagagatac cgcaccagcg ctatggctca
      721 acaatggctc gcatcggttc ttcttcactg tgaaaaaggg ttccgaagaa tcaaaggcta
      781 tagagacata ccacaggtgc tagcaaacat tgaagcaata cacactgaag ccacgcgtca
      841 ggcggcatga gagaggttga ccaaagatga tgaaaaaagg gtgccacaca tttcaactaa
      901 aaacttgaca tcttcacttt tctgcctcta tttaacaaca aatcctcgct caataaataa
      961 tctttttttg agagttatca acatgttcgc ggttcccatg aatgaaccaa tctttatatt
     1021 tatatgttag cactatttca attatgcaaa atacaagctt acttataact gtttgatatt
     1081 attgttataa attttcatgc cattaatatc aagcatcgaa ttacgttgta tatcaacaaa
     1141 tttcagaggc taccagtggc ttttaaagga tctaagtggt tcgctacccc tattcgcacc
     1201 agtcccttca tcacaggttt gcatggtctg gcaaatcctc atcatgaatt ttactatcaa
     1261 gtttgttatt gtggtgctca gtatcgtatc ggccaggata tgtggtaagg ccttttcccc
     1321 tcaacattaa agaattggtg tatcaagtat tactattgcc taaggatcag atttcgcatt
     1381 gatgtttggc ttcatattat tcgtttcaga caactacact aagcaatcct tgatgccagg
     1441 atttaagatt cggcgccgat attttggtat tcagacaata tggatagtcg ctatgaatat
     1501 actcgaagaa taggaaatac tactttcagg ccattccacg tatcattata tcgcagtctt
     1561 ttcattcacc catgatcaag accatggaac tctgtcctac gagtttttag ggtggcattc
     1621 atttctcgaa gtcaacctat ttttccaaaa atgagcatgg aaacttttcg ccatcttctt
     1681 gaacttattc accaatgaat ttgacaattt cctgggtaat cctgcattc
//
LOCUS       DAOOMC010000233         1727 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdus sp. isolate AMR_MDS_2685
            NODE_89036_length_1727_cov_4.753589, whole genome shotgun sequence.
ACCESSION   DAOOMC010000233 DAOOMC010000000
VERSION     DAOOMC010000233.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864760
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdus sp. (wastewater metagenome)
  ORGANISM  Syntrophorhabdus sp.
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae; Syntrophorhabdus.
REFERENCE   1  (bases 1 to 1727)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1727)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,729
            Genes (coding)                    :: 2,708
            CDSs (with protein)               :: 2,708
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 7 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1727
                     /organism="Syntrophorhabdus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_89036_length_1727_cov_4.753589"
                     /isolate="AMR_MDS_2685"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2699756"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            195..1562
                     /locus_tag="PK125_13765"
     CDS             195..1562
                     /locus_tag="PK125_13765"
                     /inference="COORDINATES: protein motif:HMM:NF033517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HPB39212.1"
                     /translation="MEKSDKIIDDLQRIILMLSRENTQMKARIEVLERELSRYKTKKD
                     SSNSSLPPSKDDNRPLRTSSLREKGGRKAGGQPGHDGNTLKMTDNPDEIVEHRACFCP
                     ECGKDVSSQPFEYFGKRQVIDIPVIKQIVTEHRVYRCTCTCGKVVESDFPVDVDSPVK
                     YGKGIESLIGYLSVRQYLPFKRLQEMLSDIFAVQISEGGLHCLLKRLASKGVDAYEMI
                     RQNVLSSGVIGTDETGVKVNGKKHWFWTWQNDRATYIAASDNRGTNTINENMSGISGE
                     AVLVHDCWKAHFQTPVGSHQLCTAHLERETKYLEERYKEDWPVRFRTMLKKAHELKKQ
                     LTPPLYYYPNQLRADLENELEGLLHESLDPRHKEMISFQKRITKYRDHVFTFLYHPDV
                     PANNNASERAIRNVKVKQKISGQFKILSAAENFAILRSIIDTAIKNKQNVVGALSVIA
                     AYKRN"
BASE COUNT          528 a          345 c          395 g          459 t
ORIGIN      
        1 ttgtaactaa tcagtttctg taaaattggg tgttattaac aattcttccc gtttgaatca
       61 acatttttcg tctttgtgct actccgtttt gcaaaaatga acacatatga acaattatta
      121 ttttagtgtt tgataaataa gcgattacgt tctttgatat attggatttc aggattagtt
      181 tcgtagtgaa gactatggaa aaatccgata aaatcattga cgatcttcag cgcatcattt
      241 tgatgctctc tcgggagaac actcagatga aagcacgtat cgaggtactt gagagagagc
      301 tgtcgcggta caaaaccaaa aaggacagca gtaacagctc tttgccacct tcgaaagatg
      361 ataacagacc actgcgtacc agcagtctca gggagaaagg cggtcgtaaa gcaggaggac
      421 agcccgggca tgatggcaac actcttaaga tgacggataa tccggatgag attgttgaac
      481 atcgtgcatg tttttgtcct gaatgcggga aagacgtgag cagtcagcct tttgagtatt
      541 ttggaaagag acaggtcatt gatatccccg tgattaaaca gatagttacc gaacaccggg
      601 tttacaggtg tacctgcact tgcggaaagg ttgtcgagag tgattttcct gtcgatgttg
      661 acagtcctgt taaatatggt aaaggcattg agtctctcat tggttatctt agcgtaaggc
      721 agtaccttcc cttcaaaagg cttcaggaaa tgctcagtga catatttgct gttcagataa
      781 gtgaaggggg acttcattgt ttgttgaaac gtcttgcatc caaaggcgtt gatgcttacg
      841 aaatgatacg gcagaatgta ttgagcagcg gagttattgg cacagacgaa acgggagtga
      901 aagtcaacgg gaaaaaacat tggttctgga catggcaaaa cgaccgggcc acctacattg
      961 ctgcttctga caaccggggc acaaatacaa tcaatgaaaa catgagcgga atctccgggg
     1021 aagctgtcct tgttcatgat tgctggaagg ctcactttca aaccccggta ggcagtcatc
     1081 agctttgcac ggctcatctg gaacgtgaga ccaaatacct tgaggagcgt tacaaagagg
     1141 actggccggt cagatttcgg accatgctca aaaaagcaca tgaacttaaa aaacaactta
     1201 cgcctccgct ctattattat ccgaaccaac ttcgagctga cctggaaaat gagctggagg
     1261 gtctcttgca tgaatctttg gacccaaggc acaaagagat gatatctttt cagaagagaa
     1321 ttacaaaata ccgtgatcat gtcttcacct ttctttatca tcctgatgta cctgcaaata
     1381 ataatgcttc ggaaagagcc atacgcaatg tcaaggtcaa acaaaaaata tccggacagt
     1441 tcaaaatact gagtgctgct gaaaacttcg ccatcctgag atccataatc gatactgcca
     1501 taaaaaataa acaaaatgtg gttggagcat taagtgtcat cgctgcctat aaaaggaact
     1561 gattcattct attcttatta actttaggca ctccaaactc taggcactgt gattagttac
     1621 aaaaaaagca gctaattaat aatcttataa ctgcttgatt ttctggtagc gagaggggaa
     1681 atagcctgcg gcgattcccg gagggggtgc ttaacaacgg gaagccc
//
LOCUS       DAOOMC010000234         1695 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdus sp. isolate AMR_MDS_2685
            NODE_91532_length_1695_cov_9.348171, whole genome shotgun sequence.
ACCESSION   DAOOMC010000234 DAOOMC010000000
VERSION     DAOOMC010000234.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864760
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdus sp. (wastewater metagenome)
  ORGANISM  Syntrophorhabdus sp.
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae; Syntrophorhabdus.
REFERENCE   1  (bases 1 to 1695)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1695)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,729
            Genes (coding)                    :: 2,708
            CDSs (with protein)               :: 2,708
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 7 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1695
                     /organism="Syntrophorhabdus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_91532_length_1695_cov_9.348171"
                     /isolate="AMR_MDS_2685"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2699756"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1077
                     /locus_tag="PK125_13770"
     CDS             <1..1077
                     /locus_tag="PK125_13770"
                     /inference="COORDINATES: protein motif:HMM:NF015445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HPB39213.1"
                     /translation="MKKTTVFLALVLFSLVLFFSHEAVSAPIELKWTNYFPVPSMQSK
                     ICEQFIKELEPKLQGKVKFSYFTAGTLLTAPKIYDGVVQGIADIGFSNISYTYGRFKV
                     TELLDLPLGFPNAWVSNHVANDFFRKFKPKEWDKIHMISMHTSPANVMMTASKPVHKM
                     EDLKGMTIRGQGYIAEVAAALGGTPRVIATPETYDAVLKKVVDGIYIPMETMRAFRFA
                     EVVKYVTECWPIGQVYTFFLIMNKDTWNKLPEDVKKVFNEYPFEDKLAETWNEIDIDG
                     KKLGIEKKIEFIQLSPEESKKWIKAADTVIEKYVKTMVGEGYKEAEVRGWIKYAKERI
                     DYWTKKQRELKIKSSTGPDDIRIK"
     gene            1077..1559
                     /locus_tag="PK125_13775"
     CDS             1077..1559
                     /locus_tag="PK125_13775"
                     /inference="COORDINATES: protein motif:HMM:NF016200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HPB39214.1"
                     /translation="MEKFERFTKGLSKGFEWVGVIGMLAMFFANLVDVIGAKAFSWPL
                     PGAIEVLSFALVVTIAPAIAYGLFLGIHLRIDFIVDKFPKILRSAFAPFVSLMCLILF
                     ILLFWKGSEYAYSLQKAGEKGGSSHIPFFPFAYVLSLSCIPVILFFVLETIKSLKVKK
                     "
BASE COUNT          464 a          334 c          402 g          495 t
ORIGIN      
        1 atgaaaaaaa caactgtttt tttggctttg gttctttttt ctttggttct tttcttttcc
       61 catgaggcag tttcagcacc aatcgaacta aaatggacca attacttccc agtgccttca
      121 atgcagtcca agatctgtga gcagtttata aaggagcttg agccgaagtt gcagggcaag
      181 gttaaattct cctatttcac tgcaggaaca ctgctgaccg cacccaagat ttatgatggg
      241 gttgttcaag gtattgcaga catcggattt tcaaatatct catataccta cggacgattt
      301 aaagtgacag aactgcttga ccttcctctg ggttttccga atgcatgggt ttcaaatcat
      361 gtggccaatg atttcttcag aaagtttaaa ccaaaggaat gggataaaat ccatatgatc
      421 tccatgcaca catcccctgc aaatgttatg atgacagcat caaagcctgt gcataagatg
      481 gaagatttga aagggatgac aatacgtggc cagggttaca ttgccgaggt tgcagcggct
      541 ttgggaggaa cgccaagggt tatcgcaacg cctgaaacct atgatgcagt tctcaaaaaa
      601 gtcgtagacg gtatttatat acctatggag actatgagag ccttcaggtt cgcagaggta
      661 gtgaagtatg ttacggaatg ctggcctatc ggtcaggttt atacgttttt tctcataatg
      721 aacaaggata cctggaataa gctacctgaa gatgtaaaga aggtattcaa tgaatatcct
      781 ttcgaggaca aactggctga gacatggaat gagatcgata tagacggcaa aaaactcggt
      841 atagaaaaga agattgaatt tatccagctc tcacccgagg aatcaaagaa gtggataaag
      901 gcagcggaca cggtcatcga gaaatacgtg aagactatgg tgggtgaggg ttacaaggaa
      961 gctgaagtca ggggctggat aaaatatgca aaggaacgca tagactactg gacaaagaag
     1021 cagcgggaac tcaaaatcaa gtcgtcaaca ggacctgatg atataaggat taagtagtgg
     1081 aaaaattcga acggtttaca aagggtttga gcaaggggtt tgagtgggtc ggcgttatcg
     1141 gcatgctcgc catgtttttt gcaaatctgg ttgatgttat aggggcaaag gccttcagtt
     1201 ggcctttgcc cggggccatc gaggtgttaa gctttgccct ggtcgtcacc atagccccag
     1261 ccatagccta cggtcttttc ctgggaatcc acctgaggat agattttatc gtggacaagt
     1321 ttcccaaaat ccttcggtct gcatttgctc cttttgtatc cctgatgtgc ctcattctct
     1381 tcattttact cttctggaag gggtccgaat atgcatattc tcttcagaaa gccggcgaga
     1441 aggggggttc atctcatatc cccttctttc cttttgccta tgtgctctct ttgagctgca
     1501 ttcctgttat cctgtttttt gtccttgaaa caataaaatc tctgaaggta aaaaaatgag
     1561 ttcgactacg attggtatac tgggtattat tatcgttgtg gcgtttttta ttatccgaat
     1621 gcccatcgcc ttttcaatgg cctttgttgg ctttttaggc ttttcactgg ttacatcctt
     1681 taagacagga ttgtc
//
LOCUS       DAOOMC010000235         1674 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdus sp. isolate AMR_MDS_2685
            NODE_93304_length_1674_cov_7.861643, whole genome shotgun sequence.
ACCESSION   DAOOMC010000235 DAOOMC010000000
VERSION     DAOOMC010000235.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864760
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdus sp. (wastewater metagenome)
  ORGANISM  Syntrophorhabdus sp.
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae; Syntrophorhabdus.
REFERENCE   1  (bases 1 to 1674)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1674)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,729
            Genes (coding)                    :: 2,708
            CDSs (with protein)               :: 2,708
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 7 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1674
                     /organism="Syntrophorhabdus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_93304_length_1674_cov_7.861643"
                     /isolate="AMR_MDS_2685"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2699756"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..233)
                     /locus_tag="PK125_13780"
     CDS             complement(<1..233)
                     /locus_tag="PK125_13780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB39215.1"
                     /translation="MKSINWNTEKSVALKASRGICFEDVVFFIERGDILDDCLHPNQK
                     DYPGQRIMVINIDNYAYLIPYVENDEELFLKTI"
     gene            complement(348..794)
                     /locus_tag="PK125_13785"
     CDS             complement(348..794)
                     /locus_tag="PK125_13785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB39216.1"
                     /translation="MEVQEDFKELLELFNGHKVEYIIVGAYALAYHGAPRFTGDIDIL
                     VKLSLENAKRILSALSDFGFGSLNLTIEDFQNPDSVVQLGVPPVRIDIITSITGVTWQ
                     EADKGKLGGVYGDIPVYFLGREQYIANKRAIGRKKDLADLESLGEE"
     gene            complement(1020..1439)
                     /locus_tag="PK125_13790"
     CDS             complement(1020..1439)
                     /locus_tag="PK125_13790"
                     /inference="COORDINATES: protein motif:HMM:NF013098.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HPB39217.1"
                     /translation="MKPRISMITLGVTDMERAIRFYEKGLGLPRMPFEGGAAFFMLNG
                     SWLSLYPWVALAEDATVSENGSGFRGITLAHVVSSKEEVAEVLNQAVRSGGKLMKPAQ
                     DVFWGGFSGYFADPDGHLWEVAWNPHFWPGPKDDEKA"
     gene            complement(1470..>1674)
                     /locus_tag="PK125_13795"
     CDS             complement(1470..>1674)
                     /locus_tag="PK125_13795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB39218.1"
                     /translation="TSCSSLFHSYNPKNEYLFGHISGGVDRDGKAFITIYGGLDPDGL
                     SFQDIIVGLTVVKRQCTRSLRSR"
BASE COUNT          483 a          400 c          323 g          468 t
ORIGIN      
        1 atgatggttt tcagaaatag ttcttcatca ttctcgacat aaggaatgag ataagcgtaa
       61 ttgtcaatat tgataaccat aattcgctgt ccaggataat ctttctgatt gggatgcagg
      121 cagtcatcta atatgtcacc cctttcaatg aaaaatacca catcctcaaa acagatgccc
      181 cgagacgcct taagtgcaac actcttttcc gtattccagt ttatactttt catgttttat
      241 attagcacat tgtgtgcact atggaaacac atatttaagg ccattgacaa aatcagccgg
      301 agcttaacgt tcggctgtat tgcttttgct cggcttttat tgttagatta ttcttctcca
      361 agagattcaa ggtctgctaa atcttttttc cgaccaatag cccgtttatt ggctatgtat
      421 tgttctctgc caaggaagta aacaggaata tctccgtata cccctcccag cttcccttta
      481 tcagcctctt gccatgtaac accagttatt gaagtgatga tgtcaatgcg aactggagga
      541 actccgagct ggactacaga atcaggattc tgaaaatcct caattgtcag gttcagcgaa
      601 ccaaaaccaa aatcagataa agcggataag attctctttg cattctcgag cgaaagttta
      661 actaaaatat caatatcgcc ggtaaaacgt ggcgctccat ggtaagcgag agcataagcc
      721 ccaacaatta tgtattcaac tttatgcccg ttgaataact ctagcaactc tttgaagtct
      781 tcctgtactt ccataatatt gctttctcag ataatcgact gttgcaacgc gttcttccgc
      841 aggtcggctt aaccagtaca agagatcgac cttggcagat tggagatgat aaagactact
      901 tttgtgaaca actttattaa tcataattga tacttatcac aaaatcgtgg atatttctat
      961 aatctctcac cgaacgggaa aatcagcagc cgaggtacga gatcgactgc aagccgtggt
     1021 tatgccttct catcatcctt tggtccaggc cagaaatggg gattccacgc aacttcccaa
     1081 agatgtccgt caggatctgc gaagtaccct gagaaaccac cccaaaatac atcctgggct
     1141 ggtttcatca gctttccacc ggagcgaaca gcttgattga gaacttctgc tacctcttct
     1201 ttgctcgata ctacatgggc cagcgtgatc cctctgaaac ctgatccatt ctctgaaaca
     1261 gtagcatctt cagcaagcgc aacccatgga tataaggaca gccatgatcc gttcaacata
     1321 aaaaaggccg cacctccctc aaaaggcatg cggggaaggc ccagcccttt ttcgtagaac
     1381 cgaattgccc tttccatatc tgtaacacca agcgtaatca tactaatgcg tggtttcatt
     1441 ctcaattcct ttcgtgcata acgaaaaaat taccgggagc gtaacgatcg ggtgcattga
     1501 cgttttacta ccgtcaaacc tacaataata tcttgaaaac taaggccatc cgggtccaac
     1561 cctccgtaga tagtgatgaa tgctttcccg tcccggtcca cgccgccgga aatgtggccg
     1621 aacaaatatt cgtttttcgg attataagag tgaaataaag aggaacatga ggtg
//
LOCUS       DAOOMC010000236         1670 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdus sp. isolate AMR_MDS_2685
            NODE_93649_length_1670_cov_7.894118, whole genome shotgun sequence.
ACCESSION   DAOOMC010000236 DAOOMC010000000
VERSION     DAOOMC010000236.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864760
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdus sp. (wastewater metagenome)
  ORGANISM  Syntrophorhabdus sp.
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae; Syntrophorhabdus.
REFERENCE   1  (bases 1 to 1670)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1670)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,729
            Genes (coding)                    :: 2,708
            CDSs (with protein)               :: 2,708
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 7 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1670
                     /organism="Syntrophorhabdus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_93649_length_1670_cov_7.894118"
                     /isolate="AMR_MDS_2685"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2699756"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1340)
                     /locus_tag="PK125_13800"
     CDS             complement(<1..1340)
                     /locus_tag="PK125_13800"
                     /inference="COORDINATES: protein motif:HMM:NF017021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF748 domain-containing protein"
                     /protein_id="HPB39219.1"
                     /translation="MPKWRTILFWIAGLFLFFTIAGFFLVPPIAKHLLIKNLSEALQR
                     DVSIQQIKANPYNLTVVVRGFAIKEKETSETILSFEEFFIDIHALSIFKRAIILSKVS
                     LTKPYIKVIRNEDGSYNWSDLVEKTSEGPSPPAKKSKPVQFSINNIVVSGGTIDFLDA
                     PKEVFHEVTDLNLAVPFVSNMKYHVQTFIQPRFSALINDDPFTIEGKTKPFADSLETV
                     FDININNLDLADYLAYVPNKKHFNLLSGSLNLIAKVSYIQHAEKKPTLEVTGKMTLND
                     LNLADDKKNPLLKVGVVDFEIASVKPLEDLIHISRLGISSPDINMRRKKDGTIDIVTI
                     TTLLIGTAHQKEDANSSHRAQDKNPMSLLIDVWQVKDGKMKFHDSMTQEPVTIEAYGI
                     VLDAKGFSLEKGSKTDFQFSFLLGKTGKVNGKGTIGLEPIAANLSLDMKGVGINT"
     gene            complement(1451..1651)
                     /locus_tag="PK125_13805"
     CDS             complement(1451..1651)
                     /locus_tag="PK125_13805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HPB39220.1"
                     /translation="MAKGTVKWFNESKGFGFITKDDGSDVFVHYSAIQGNGFKSLTEG
                     ETVTFDVVTGPKGPAASNVVKG"
BASE COUNT          468 a          331 c          349 g          522 t
ORIGIN      
        1 gtatttatac ccaccccttt catatcaaga gatagatttg ctgcaatagg ttcaaggcct
       61 attgtccctt ttccattaac tttacctgtc ttccccagaa gaaaggaaaa ctggaaatct
      121 gttttactcc ccttttcgag agaaaagccc ttagcatcta agacaatgcc ataggcctct
      181 atggtaacag gttcctgtgt catggaatcg tgaaatttca ttttcccgtc tttcacttgc
      241 catacatcaa ttaggaggga cattggattt ttgtcctgtg cccggtgtga tgagttggcg
      301 tcctcttttt ggtgggctgt tcctatgagc agggttgtga tggttacaat atcgatggtc
      361 ccgtcttttt tacgcctcat attgatgtcg ggagaactta tacccagtct tgatatatga
      421 attaagtctt ccaggggttt aactgaggcg atttcaaaat caactactcc aacctttaaa
      481 agaggatttt ttttgtcatc tgcaaggttg agatcattca gagtcatttt gccagtcact
      541 tcgagggtcg gttttttctc tgcatgctgg atataagaaa cctttgcgat taaattcaaa
      601 gaacctgaaa ggaggttaaa atgtttttta tttggcacat aagctaagta gtctgcaaga
      661 tcaaggttgt taatattgat atcaaaaact gtttccaaag agtctgcaaa aggttttgtc
      721 ttcccttcta tagtaaaggg gtcatcgttt atcagtgcag aaaatctggg ttgaataaag
      781 gtttgtacat gatatttcat gttggacaca aaaggaacgg caagattcaa atctgttact
      841 tcatgaaaga cctcttttgg agcatcgaga aagtcgatgg taccccctga aacgactatg
      901 ttgtttattg aaaactgtac aggtttagat ttctttgctg gaggtgacgg tccttcagaa
      961 gtcttttcaa ccaaatcact ccaattgtat gacccgtcct cgttacggat tacttttatg
     1021 taaggtttag ttaaactgac cttgcttaga atgatagctc gtttgaagat agatagtgca
     1081 tgtatatcga tgaaaaattc ctcgaaggaa agtattgttt cagacgtttc cttttctttt
     1141 atggcaaaac ccctaaccac aaccgtcagg ttgtatggat tcgctttaat ctgttgaatt
     1201 gacacatctc gttgcaaagc ttcagacagg ttttttatta gcagatgttt tgctatgggg
     1261 ggaacaagaa aaaaacctgc aattgtgaaa aacaggaata atcctgcaat ccagaatagg
     1321 atagtcctcc attttggcat aacagtataa tagtatggaa ttgatactga aaaaaccaga
     1381 gaaaaaactt gaaagaggga gtgtcttcga taaaaacggg cccggttggt gccgggcccg
     1441 caataaaggc ttagcctttt acaacattcg acgctgccgg gccctttggg ccagtgacaa
     1501 catcaaaagt cactgtttcg ccctcagtca gggatttaaa accgttgccc tgaatggctg
     1561 agtagtgaac aaaaacatcg ctgccatcgt ctttggtgat gaaaccaaag ccctttgact
     1621 cattgaacca cttcacagtt cctttcgcca tgctgactac ctccttgtta
//
LOCUS       DAOOMC010000237         1666 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdus sp. isolate AMR_MDS_2685
            NODE_94031_length_1666_cov_4.333954, whole genome shotgun sequence.
ACCESSION   DAOOMC010000237 DAOOMC010000000
VERSION     DAOOMC010000237.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864760
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdus sp. (wastewater metagenome)
  ORGANISM  Syntrophorhabdus sp.
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae; Syntrophorhabdus.
REFERENCE   1  (bases 1 to 1666)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1666)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,729
            Genes (coding)                    :: 2,708
            CDSs (with protein)               :: 2,708
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 7 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1666
                     /organism="Syntrophorhabdus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_94031_length_1666_cov_4.333954"
                     /isolate="AMR_MDS_2685"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2699756"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1645)
                     /locus_tag="PK125_13810"
     CDS             complement(<1..1645)
                     /locus_tag="PK125_13810"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HPB39221.1"
                     /translation="MNSTNDSDSSKKRYLFDPHIDPELQFDSSRAEVEKLLNEALSSD
                     SIDEIKGYLSKLQKMQDPFLNWAGKAERTSFDVPVVSLHIHERISPGSVLNSITTSTV
                     EYSHGLFGEIEKRPFREAIQFYKHKQNWKNRLIAGDSLLVMNSLIQKEAMAGQVQMVY
                     IDPPYGIKYGSNFQPFVGKRDVKDGKDEDLTSEPEQIKAFRDTWELGIHSYLSYLRDR
                     LLLARELLADSGSCFVQISDENVHLVRNLMDEIFGKENFVSLITFATTTGFQTTTLSR
                     SGDYICWYSKTKSVKSKQLFLKKEIGGSASDHYDYMEHHNGELIPFDENVYNNESFNL
                     VTKSQLLSQGQSNSEQPFEFSNIMHKPKTGSHWKAQYPEGMLRLRKSNRLCVSGNTLR
                     YKRYFKDYFYYQITNFWNEQMSEQNKAYVVQTSDKVIERCILMTTDPGDLVLDITCGS
                     GTTAFCAEKWGRRWITCDTSRVALAIARQRLATAMFDYYTLKDDKLGVDGGFIYKTVP
                     HITLKSIANNEEIDRIWDKHHPHIEKALSDLNKAFVNSSF"
BASE COUNT          442 a          355 c          337 g          532 t
ORIGIN      
        1 tgaaagagga atttacaaaa gctttgttca ggtcagaaag ggccttttca atatgtggat
       61 ggtgcttgtc ccagatcctg tcaatctctt cgttattggc aatggatttc aaggtaatgt
      121 gcggaacggt cttgtagata aaaccgccat ccacaccaag cttgtcgtcc ttgagtgtgt
      181 aatagtcaaa catggcggtg gcaagcctct gcctggcaat ggcaagtgca acacgggagg
      241 tgtcgcaagt gatccagcgc ctgccccatt tttcagcaca gaaggctgtt gtgccagaac
      301 cgcaggtaat gtcaaggaca aggtcgcctg ggtcggtggt cataaggata cagcgttcaa
      361 taactttatc agatgtctga acaacatatg ctttattttg ttcagacatt tgttcattcc
      421 agaaattagt tatttgataa taaaaataat ctttaaaata ccttttatat cttaatgtgt
      481 tgcctgaaac gcatagtcta ttagattttc taagtcgtaa cattccttct gggtattgcg
      541 ctttccagtg tgaaccagtt tttggtttat gcatgatatt agaaaattca aacggttgtt
      601 cagaattgga ttgaccttgt gacaaaagct gagattttgt cacaaggtta aaagattcgt
      661 tgttatatac attctcatca aatggtatta attctccatt gtgatgttcc atataatcgt
      721 aatgatctga tgctgaacca ccaatttcct tttttaggaa taattgtttt gattttacag
      781 attttgtttt tgagtaccag cagatatagt cccctgatct agaaagtgtg gttgtttgaa
      841 atcctgttgt agtagcaaaa gttattaaac tcacaaaatt ctctttccca aatatttcat
      901 ccatcaggtt cctcaccagg tgcacattct catcacttat ctggacaaag caggaaccac
      961 tatctgcaag cagttccctg gctaacagta atctgtctct taggtacgaa agataggaat
     1021 gtatccccag ttcccaggtg tccctgaagg cctttatctg ttccggctca ctggtcaggt
     1081 cttcgtcttt gccatccttc acatctctct tgcccacaaa gggctggaaa ttgctcccgt
     1141 attttatgcc atacggtggg tcaatataaa ccatctgcac ctgcccggcc attgcctcct
     1201 tttgtatgag cgagttcatt acaaggagcg aatcgccggc tatcagcctg tttttccaat
     1261 tctgcttgtg tttgtaaaac tgtattgctt cccgaaacgg ccttttttca atctccccaa
     1321 aaagcccatg gctatactca accgtactcg tggttataga attcaggact gagcctggac
     1381 ttatcctttc atggatatga agggacacaa caggcacatc aaaacttgtt ctttcagcct
     1441 tgcctgccca gttcaaaaat gggtcctgca ttttttggag ttttgaaaga tagcccttta
     1501 tttcatcaat ggaatctgaa gaaagggctt cattgagcaa tttttccact tctgccctgg
     1561 aagaatcaaa ttgaagctca ggatcaatgt gcgggtcaaa caagtacctt tttttggaac
     1621 tgtcagaatc atttgttgaa ttcaccagac caacatccgg gttgtt
//
LOCUS       DAOOMC010000238         1563 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdus sp. isolate AMR_MDS_2685
            NODE_103644_length_1563_cov_7.055040, whole genome shotgun
            sequence.
ACCESSION   DAOOMC010000238 DAOOMC010000000
VERSION     DAOOMC010000238.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864760
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdus sp. (wastewater metagenome)
  ORGANISM  Syntrophorhabdus sp.
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae; Syntrophorhabdus.
REFERENCE   1  (bases 1 to 1563)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1563)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,729
            Genes (coding)                    :: 2,708
            CDSs (with protein)               :: 2,708
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 7 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1563
                     /organism="Syntrophorhabdus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_103644_length_1563_cov_7.055040"
                     /isolate="AMR_MDS_2685"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2699756"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            138..392
                     /locus_tag="PK125_13815"
     CDS             138..392
                     /locus_tag="PK125_13815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB39222.1"
                     /translation="MKTTKSGKGLKEFPSWNQFVAILFLPVRPGSLIVRNLRLASCMG
                     KPKYIGIDNASYRSTLSCVNEKRPWRLYEAVFYRLLERYG"
     gene            389..559
                     /locus_tag="PK125_13820"
     CDS             389..559
                     /locus_tag="PK125_13820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB39223.1"
                     /translation="MIAPTKKKFCFRNRRSNLIAMVRYNLFTYRNLWGWIDCPPEVPP
                     IIPYREQLALEF"
     gene            complement(1007..1207)
                     /locus_tag="PK125_13825"
     CDS             complement(1007..1207)
                     /locus_tag="PK125_13825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB39224.1"
                     /translation="MGVLTSTVGTGDIGKTELARMYAKKYGTHYSLDTTGEGKIKFIT
                     LDPSKHYQRQRPLFNQCALLFI"
     gene            complement(1350..>1563)
                     /locus_tag="PK125_13830"
     CDS             complement(1350..>1563)
                     /locus_tag="PK125_13830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB39225.1"
                     /translation="VGVFGLDETLAELFSEGWQANDEAAAEIIKRLEAHKNYIPASER
                     AHKEYAYILLKEYKKYIKVQAAKKKQ"
BASE COUNT          426 a          342 c          340 g          455 t
ORIGIN      
        1 ttttcagggg caactccaaa aggtacagct aattatccca ggatttaaac gaaaccccaa
       61 ggaggtaccc atgaataagt ttagcaacat gttcggccag gcactacaga gattctcaag
      121 aaggagctct attggcaatg aaaacgacaa agagtggaaa gggattaaaa gaattcccct
      181 cgtggaatca gtttgtggcc atactctttc tgccagttag gccaggctca ctcattgtga
      241 gaaatctgcg tcttgcaagc tgtatgggga aaccgaaata tatcggtatt gataatgcct
      301 cttatcgttc caccctctcc tgtgtcaatg agaagaggcc atggagactc tatgaggcag
      361 tcttctatcg gcttttagaa aggtacggat gattgctccc acgaagaaga agttctgttt
      421 cagaaatagg cgctccaacc tcattgccat ggtccgttac aatctcttta cctatcgtaa
      481 tctctggggg tggattgatt gtccccctga ggtgccgccc atcataccct acagagaaca
      541 gcttgcattg gagttttagt atggtggaca gcaaaatgaa gcatgctaag cacaagaaac
      601 accaaccaga ttttaacatg gaggatttac ccgccctaaa ccattgttgt attaagggaa
      661 aaatcaggag gttggattat cttggacggc agtgtagctt cttatctctt taatcctggg
      721 tttgacaata ttccgaatca gacagggata aattattgtt gaaagagcat aaaataggcc
      781 cccaagatac ctttcaggca atgggtttga tctgtatttg ccgtacccac actttgactt
      841 tgacgaaaca ttcaatagtc tggctaaaaa caaaggaaac cccagaatct ttgcgctccc
      901 gatacgggtt aaattgaaag tccattcatc ttctgtcggt tgaagaaata taaagttact
      961 gcttttcaga tagcgttggt ttaacggaat ccatttaaca atgttgtcat ataaagagca
     1021 gtgcgcactg attaaacaaa ggccgttgcc gttgataatg ttttgatgga tcaagtgtga
     1081 taaatttaat tttcccctca ccggttgtgt ccagggaata atgggttcca tacttttttg
     1141 catacatacg ggcaagctct gttttaccaa tgtcgcccgt tccaactgta ctggttagta
     1201 cgcccatttt tcaattcaca ccatgacctg atgaatccct tcatgcttgc tttacctgcc
     1261 aacaaaggtg tttaaatgct catgggccat gaaacagact cgggcggctt tttctaaccg
     1321 gtctgagagg tgataaggat tgggtgtttt tattgttttt tcttggccgc ttgtaccttg
     1381 atatattttt tgtactcttt cagaaggata tatgcatact ctttatgagc acgctcagat
     1441 gcgggaatat agttcttatg tgcctccagt ctcttgatga tctctgctgc tgcctcatcg
     1501 tttgcctgcc agccttccga gaaaagctcg gccagcgtct catcaaggcc aaacacaccg
     1561 acc
//
LOCUS       DAOOMC010000239         1562 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdus sp. isolate AMR_MDS_2685
            NODE_103761_length_1562_cov_5.419376, whole genome shotgun
            sequence.
ACCESSION   DAOOMC010000239 DAOOMC010000000
VERSION     DAOOMC010000239.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864760
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdus sp. (wastewater metagenome)
  ORGANISM  Syntrophorhabdus sp.
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae; Syntrophorhabdus.
REFERENCE   1  (bases 1 to 1562)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1562)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,729
            Genes (coding)                    :: 2,708
            CDSs (with protein)               :: 2,708
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 7 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1562
                     /organism="Syntrophorhabdus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_103761_length_1562_cov_5.419376"
                     /isolate="AMR_MDS_2685"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2699756"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            145..1461
                     /locus_tag="PK125_13835"
     CDS             145..1461
                     /locus_tag="PK125_13835"
                     /inference="COORDINATES: protein motif:HMM:NF025042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HPB39226.1"
                     /translation="MNYRGREITKDIFYAALKAVDPYASVQHHIPHILSTYQNGRFNN
                     LFLVSFGKAAFLMAKAISDQAPQLVTHGIVITKYGHLLAENLHANIDLYEAGHPIPDE
                     NGLIATKKAISMLKVADEKTLVLCLISGGGSALLTAPKGMISLDDKQKATELLLKAGA
                     SINELNTVRKHISEIKGGRIAEYAYPARIISLILSDVIGDQLDVIASGPTTPDSTTYR
                     MAHDVVEKYQLAEKLPKKVLDVLKKGVEGVFPDNPKEGNHIFEGVENIIIGSNRKAAL
                     AAMEKAKELGFDATIISTEIQGEAIDVACWLARNAIEAKQLLKEKDGKKICLISGGET
                     TVTVKGNGKGGRNTELALAFALEVAGKKDIVLLSAGTDGTDGPTDSAGAIVTGETIER
                     APSLKLDPRAYLVNNDSYTFFSKTGELLITGPTGTNVMDLQIILIG"
BASE COUNT          488 a          342 c          344 g          388 t
ORIGIN      
        1 atttatatca ggaaaattga acacactgtg atatgataag gaactcattc ataatgacct
       61 gtagtattga ttgtacaggc tgaagagata aaggctgcta aatttgctct tataatcctg
      121 gagagggatc ttattctaaa ttaaatgaac tatcgcggaa gggaaattac aaaagacata
      181 ttttacgctg cattgaaggc agtcgatccc tatgcttctg tgcaacacca cattcctcac
      241 atcctttcta cctatcaaaa tggacggttc aacaacctct ttcttgtcag ttttggcaag
      301 gctgcattcc tgatggcaaa agctatttct gatcaggctc cgcaacttgt aacccacggc
      361 attgtaatca caaaatatgg ccatctcctt gccgaaaatc tccatgcaaa tattgatctt
      421 tacgaggcag gtcatccaat tcctgacgaa aatggcttaa tcgcaacaaa gaaagctatc
      481 tcaatgctta aagtggcaga cgagaaaacc cttgttctct gtctcatatc cggtggcggc
      541 tctgcactcc tcactgcgcc aaaagggatg atatctcttg acgacaagca aaaagcaaca
      601 gaactcttgc tcaaggccgg tgcgtccata aacgaactga atactgtaag gaaacatatt
      661 tcagagatca agggcggcag gattgccgaa tatgcatacc ctgcacggat aatctctctc
      721 atactctcag acgttattgg agaccagctc gacgttattg cgtcaggccc cacaacgcca
      781 gacagtacca cctatagaat ggcccatgat gtggttgaga aatatcaact ggctgaaaag
      841 ctcccaaaaa aggttttgga tgttttaaaa aaaggagttg aaggtgtatt tcccgataac
      901 ccgaaagagg ggaaccatat ctttgaagga gttgaaaaca tcatcatagg aagcaataga
      961 aaagctgctc tcgcagccat ggaaaaggca aaagagcttg gatttgatgc gacgattatt
     1021 tcgaccgaaa tccagggtga ggctatagat gttgcatgct ggctagccag gaacgcaatc
     1081 gaagcaaaac aactgctcaa agaaaaagat ggcaagaaga tttgtctcat atcaggggga
     1141 gagactacag tgactgttaa gggcaatggc aaaggaggaa gaaacacaga actcgccctt
     1201 gcatttgccc ttgaagttgc tggcaagaaa gatattgtgc tcctctcagc aggcactgat
     1261 ggcacagacg ggccaacaga ttctgccggt gcgattgtga ccggagagac catagaaaga
     1321 gcaccatcat taaagctaga tccgagggct tatcttgtga acaacgattc ttacacgttc
     1381 ttcagtaaaa caggagaact tcttatcacc ggtccaacag gtacgaatgt catggatctt
     1441 cagattatcc ttataggata gtcaattatc cacctgagac tgactcaaca gtttagggat
     1501 aagatgaagc attgctttat tccacccctc aggtcctatg ccttcagcct taatgggttt
     1561 tg
//
LOCUS       DAOOMC010000240         1558 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdus sp. isolate AMR_MDS_2685
            NODE_104142_length_1558_cov_5.649368, whole genome shotgun
            sequence.
ACCESSION   DAOOMC010000240 DAOOMC010000000
VERSION     DAOOMC010000240.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864760
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdus sp. (wastewater metagenome)
  ORGANISM  Syntrophorhabdus sp.
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae; Syntrophorhabdus.
REFERENCE   1  (bases 1 to 1558)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1558)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,729
            Genes (coding)                    :: 2,708
            CDSs (with protein)               :: 2,708
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 7 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1558
                     /organism="Syntrophorhabdus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_104142_length_1558_cov_5.649368"
                     /isolate="AMR_MDS_2685"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2699756"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1363)
                     /locus_tag="PK125_13840"
     CDS             complement(<1..1363)
                     /locus_tag="PK125_13840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB39227.1"
                     /translation="MEQSHQPFDLSSARDILTIIFKHKYIILITFLIISAGVTLYAFS
                     LKRVYESQSVLLVKLGREFTSRPEVGGDTGRGFSVPPETIMRAEMSLITSRDLINTVI
                     KTVGQEKIFPVLSKAIPGSISPDDLAINLFQENLSVNNIQGSSMIQIAFTHPDPYIAA
                     NVVNTLVDALKIKHLEVFSTDSTPFLKSQEKVFQERLKESETKLASLKQKYGVFSFEE
                     QKTALIQQRSTLDTNLKAAQSQVNELEQKVAMIKNPKWAPEVPPEIRAQLIALQQRER
                     EVMGRYIDGTVPVQNVRKEMDAVKESLKKQSEEIRQTEIHKAEGELNMARVRADNARR
                     QLGQVEAALQSLETRALELQGAKREAALQEQNYQNYSRKLEESMIMDDMDRRKMVAIT
                     IVEKAVASSLPKQGKANKLVVILMGLFGGIAAGLAIAFLLEFIAPGMTTPWSAERRLN
                     LPVL"
BASE COUNT          373 a          365 c          335 g          485 t
ORIGIN      
        1 acaaaactgg aaggttgaga cgcctctcag cgctccacgg tgtagtcatg cccggagcaa
       61 tgaattcgag gagaaaggcg atggcaagac ccgcagcaat acctccaaaa agtcccatga
      121 gtataaccac taacttgttt gctttgcctt gcttgggtaa agaacttgca acagctttct
      181 caacgatagt gattgccacc atttttcgtc ggtccatgtc gtccataatc atggattctt
      241 ccagcttccg tgaatagttc tgatagttct gttcttgcaa ggctgcctcg cgttttgctc
      301 cctgcaattc aagggcgcgg gtctcaaggg attgcaatgc ggcttcaacc tgtccgagct
      361 ggcgcctcgc gttatcagcc cgaactcttg ccatgtttaa ttcaccttct gccttatgaa
      421 tttctgtttg tctgatttct tctgattgct tcttgaggga ttcttttact gcatccattt
      481 ctttgcgaac attttgaact ggcacggtcc catcaatata tcttcccatt acttcccgct
      541 ctctctgctg aagcgctatc aactgtgccc gtatctcagg tggaacctca ggtgcccatt
      601 tcgggttttt aatcatagcg accttttgtt ccagttcgtt tacctggctt tgcgctgcct
      661 tgagatttgt atcaagagtg cttcgttgct gaatcagcgc cgttttttgc tcttcaaagg
      721 agaaaacgcc gtacttctgt ttaaggctgg ctaacttggt ctctgattct ttcaatcttt
      781 cctgaaaaac tttttcctgg cttttcaaaa acggggtgct gtctgtgctg aatacctcaa
      841 gatgctttat ctttaaagca tcaacgagcg tgtttaccac atttgcggca atgtaaggat
      901 caggatgggt aaatgcgatc tgtatcatgc tggaaccctg gatgttgtta acgctgaggt
      961 tctcctgaaa taaatttata gctaaatcat ccggcgatat actgccgggg atggctttgc
     1021 tcaagacagg aaaaatcttc tcttgaccga ctgtttttat taccgtattg atgaggtccc
     1081 tgctggtaat caggctcatt tcagctctca taatggtttc gggtggaaca gaaaatcctc
     1141 gacctgtatc tccacccact tcgggccgag acgtaaactc ccttccaagt ttgaccagca
     1201 acacagattg agactcatag actctcttta atgaaaatgc atatagagta actcctgcgc
     1261 taatgatcag aaaggttatc aggattatgt atttatgctt gaatatgatg gtcaggatgt
     1321 ccctggcgct gctcaggtca aatggctgat gactttgttc catttatctc acacagatat
     1381 ccgtttattt gctggcaata cttgtagcag tgtcgattat accgggaagg aaatgatcag
     1441 tatcattttg catatctaat accataacac tcgttacgaa taatggcaac agcacaaagg
     1501 ctaaggtaat gtatcatttc ttgtgtcacc atacaaagat ggggtaccct ctcggcag
//
LOCUS       DAOOMC010000241         1539 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdus sp. isolate AMR_MDS_2685
            NODE_106098_length_1539_cov_8.144205, whole genome shotgun
            sequence.
ACCESSION   DAOOMC010000241 DAOOMC010000000
VERSION     DAOOMC010000241.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864760
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdus sp. (wastewater metagenome)
  ORGANISM  Syntrophorhabdus sp.
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae; Syntrophorhabdus.
REFERENCE   1  (bases 1 to 1539)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1539)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,729
            Genes (coding)                    :: 2,708
            CDSs (with protein)               :: 2,708
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 7 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1539
                     /organism="Syntrophorhabdus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_106098_length_1539_cov_8.144205"
                     /isolate="AMR_MDS_2685"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2699756"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1394)
                     /locus_tag="PK125_13845"
     CDS             complement(<1..1394)
                     /locus_tag="PK125_13845"
                     /inference="COORDINATES: protein motif:HMM:NF025038.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPB39228.1"
                     /translation="MANKLAPEALHNKCDPNMFTFNTTADIPGIPGTIGQEKALKALE
                     FGLGMDSTGFNIFALGEGGTGKMTTIMTTLKEKASKEKVPQDWCYVYNFKDPDTSIAI
                     SLEPGHGVIFQKEMDDLIKALRIEIPKAFESKEYEMQKGRIIEEFQQKQSEYFSRLDE
                     EAKTRGFAVKRGPTGVLIIPIKENGEPLSKEEFEALDEKDRKKMEETGRMFQEKLNDV
                     VRVLRDADKLVRDMLSKLERMIALDLIGPLIEVMKVKYKNYEKVSQYLDDAKEEILSH
                     LDEFRAQEEQPSPIPFLKMPKSEASSLKYTVNVIVNNGNCKGAPVIFESNPSYLNLFG
                     RIEYKVSYGMATTDFTMIKAGALHRANGGYLVIDTLDLFKNPFSYDALKRALKNKEIR
                     IEDILEQYRMISTSGLKPEGIPLNTKVVLVGNPYIYYILYGLDEESRELFKVKADFDS
                     RMDRTPASMLQYAG"
BASE COUNT          373 a          344 c          318 g          504 t
ORIGIN      
        1 aagcctgcgt actggagcat cgatgccggc gtcctgtcca tacggctgtc gaagtcagcc
       61 ttcactttaa aaagttctct tgactcctca tccagaccgt aaagtatata gtaaatatat
      121 ggatttccca cgagcacaac ttttgtattg agtggtatcc cttctggctt caatcctgag
      181 gtactgatca tgcgatactg ttcaaggata tcttcaattc tgatctcctt gttttttaag
      241 gcacgtttca acgcatcata agaaaatgga tttttgaaaa ggtcgagggt atcaattacc
      301 aggtaacctc cgttagctct gtggagtgct cctgctttaa tcatggtaaa atctgtggta
      361 gccataccat aggatacctt gtattcaatt cttccaaaaa gattgagata ggaggggttg
      421 ctttcaaata tgaccggagc ccccttgcaa ttgccgttat tgacaataac gtttacagta
      481 tatttgaggg aggatgcctc gcttttgggc attttcagga aaggaatagg ggagggctgt
      541 tcctcttggg ccctgaattc atccagatgg gagaggattt cttcctttgc atcatcaaga
      601 tactgggata ccttctcata gttcttatat ttaactttca taacctctat gagagggcca
      661 attagatcaa gagcaatcat ccgctcaagt ttggaaagca tgtctctcac aagcttatca
      721 gcatcacgga gcaccctgac cacatcattg agtttttcct gaaacatcct tccagtttct
      781 tccatttttt tgcggtcttt ctcgtcgaga gcttcgaact cctcctttga gaggggttct
      841 ccgttttcct tgataggtat aattagcaca cccgttggtc ctctcttcac cgcaaatcct
      901 cttgttttag cctcctcatc aaggcgcgaa aaatactctg actgtttttg ttgaaactcc
      961 tcaatgattc tgcctttctg catctcatat tctttggatt cgaaggcttt tggaatttca
     1021 attctcaatg ccttaatgag gtcatccatc tctttttgaa agattacgcc gtggcctggt
     1081 tccagtgata tggcgatgga ggtatcggga tctttgaagt tgtaaacata acaccagtct
     1141 tgtggcacct tttcctttga cgccttttcc ttgagcgttg tcattatggt ggtcatcttt
     1201 ccagtgccgc cctcaccaag ggcgaaaata ttaaaacctg tactatccat gcccaggcca
     1261 aattcgaggg ccttaagagc tttttcctga ccgattgtgc caggtatacc tggtatgtca
     1321 gctgttgtgt taaaagtgaa catattggga tcacatttgt tatgaagcgc ttcaggtgct
     1381 aatttatttg ccatagctgc ctccatcgat gaataaactc ttttggtgta tatttagaaa
     1441 tagtgtaatc actctgcact tttttagcca ctgccattcg tatcaggtag tgtatcatat
     1501 cttgtgtcat aaggggaaga tcgggtacct tccttacaa
//
